LOCUS       DAMJVI010000001       147539 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.001, whole genome shotgun sequence.
ACCESSION   DAMJVI010000001 DAMJVI010000000
VERSION     DAMJVI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 147539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 147539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..147539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.001"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(780..1289)
                     /locus_tag="PC545_000001"
     CDS             complement(780..1289)
                     /locus_tag="PC545_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2454801.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLSPI
                     YSNNTVIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(1500..2909)
                     /locus_tag="PC545_000002"
     CDS             complement(1500..2909)
                     /locus_tag="PC545_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454802.1"
                     /translation="MENYFDKQAKELYNKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMSREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKGELPWNLYL
                     NRIMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKSEIAVALDVSGSISDEEFKQAI
                     KEVLSIVKNYNHEITIIECDNEIRRVYKVRTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVKTNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(2913..4043)
                     /locus_tag="PC545_000003"
     CDS             complement(2913..4043)
                     /locus_tag="PC545_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2454803.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LIVIDGNLLKEGEIGGLPTIESYVGVNSNGYKTEKKTTVYAVHNKLREIDEEISKSKT
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKEKNNSIPRAVFLNVVKGNVGKVIAEEFISFIESNSEPLISYEDV
                     FLGESIDESIVERVKNESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIEFLKMY
                     PVDLMIGIMKDIRNSYIEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(4055..4858)
                     /locus_tag="PC545_000004"
     CDS             complement(4055..4858)
                     /locus_tag="PC545_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454804.1"
                     /translation="MEKLRVIEMIKNNPNIIATIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEDMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYAKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYNAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYILKKVSKDELESVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRNSESIPIDKIMFIYKYGSKTAKKIAVDEKLKIM"
     gene            complement(5240..6121)
                     /locus_tag="PC545_000005"
     CDS             complement(5240..6121)
                     /locus_tag="PC545_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HDF2454805.1"
                     /translation="MKITEGYMPFKGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIEELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSAKLWEKEQKRRISYMSEVDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGAIDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIKVLTEWLNAND"
     gene            6577..7551
                     /locus_tag="PC545_000006"
     CDS             6577..7551
                     /locus_tag="PC545_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454806.1"
                     /translation="MNNINSLKVIKLSLGCSLAIFIAWLLKLEYSMVAGVIVLLTVKD
                     TKRETLKGSIGKIYGFLLCTIFSYLCFNILGYNLTSFSIFIFIIIPLCFLLNIQDVIA
                     MCVVIASHYYLQGETSIKWILNEAGIFAVGTGIGVLINMYIPTNIHKIHEVQKKLQEE
                     VSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNINNTLLSDTRFFLEHM
                     DIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGYTEFEAETGNLLLEELKRLM
                     LSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKHAQMLRDNNFYK"
     gene            complement(7667..8728)
                     /locus_tag="PC545_000007"
     CDS             complement(7667..8728)
                     /locus_tag="PC545_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2454807.1"
                     /translation="MKKIRKLATKLLITTIVLISGMSMTVYGMTAKEVTAKAPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDIIYKSLSHPLPFDKTAYPKVGAIVQTVKPEQTQKPAPPGKHTMLP
                     HQEGAKAVGNFGKKGEDINEKHVMYEGVRCSVVNSSVNTITFTGSESNLDPSNLTIAV
                     IGFMNTPYGMFALAPEGKPVEYPFKYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIE
                     ITDEKANIGLGQIMYVRYE"
     gene            complement(9340..10191)
                     /locus_tag="PC545_000008"
     CDS             complement(9340..10191)
                     /locus_tag="PC545_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2454808.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDIAIAISYSGESKEVIKSIENAKLKGCKVICITKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNINNA"
     gene            complement(10208..11128)
                     /locus_tag="PC545_000009"
     CDS             complement(10208..11128)
                     /locus_tag="PC545_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HDF2454809.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLDHHFEQGTKIKVSYSGGVFKSGDLILEPLKKY
                     LNKTRFGIVRPVLTPDLGACLLAKKKYYSK"
     gene            complement(11238..12152)
                     /gene="murQ"
                     /locus_tag="PC545_000010"
     CDS             complement(11238..12152)
                     /gene="murQ"
                     /locus_tag="PC545_000010"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDF2454810.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAIEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVKYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKSILDRQEGYISEALKSIEKL"
     gene            complement(12251..13348)
                     /locus_tag="PC545_000011"
     CDS             complement(12251..13348)
                     /locus_tag="PC545_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2454811.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKAAENGFTRIFSCLLSVKDTK
                     ENIIKKFKRINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKTKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEINMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLKH
                     FIKCTERFNKYGLRTAVFVTSQNETTFGPWPVTDGLPTIEMHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DSLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(13581..14927)
                     /locus_tag="PC545_000012"
     CDS             complement(13581..14927)
                     /locus_tag="PC545_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2454812.1"
                     /translation="MLDKFTEFLDTKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYLKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPELLKIVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEAY"
     gene            complement(15016..15324)
                     /locus_tag="PC545_000013"
     CDS             complement(15016..15324)
                     /locus_tag="PC545_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2454813.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGAKNGIDIDVLAVGTQEFENE
                     VKNGWDIAMVAPQVRHRFDYLKAFADEVSVPCALIQAQAYSPLGGPKLLKQVQELLSK
                     "
     gene            complement(15586..15984)
                     /locus_tag="PC545_000014"
     CDS             complement(15586..15984)
                     /locus_tag="PC545_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454814.1"
                     /translation="MSNNKKKKRKKNNNYKNAKSTKSNKNTKKQMEKNQRTDYNIPTI
                     SFKSGLILIVSSLVSTVVFPLLLSIFGIDNRFGVVIGNSLITSFAVAYTRYFVETKRG
                     FSLGFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(15986..17146)
                     /locus_tag="PC545_000015"
     CDS             complement(15986..17146)
                     /locus_tag="PC545_000015"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDF2454815.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGNGRNTKVKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCHKANFHIANLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAYETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNLALQNIGGIGNITIIPKSCNIDDVFAFDTGPGNMIIDGVCQRLFNRKYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQAPPKSTGREVFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIGYIKELLLEVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKNKVILGNITPKPFGGK"
     gene            complement(17577..18596)
                     /locus_tag="PC545_000016"
     CDS             complement(17577..18596)
                     /locus_tag="PC545_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2454816.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNKYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYTNLYNCIENSGEIDSYLVDGQSFEENLIKDIIENLK
                     IKNNSDIETYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSSIEDSLDIKNQVAKI
                     NFLVNSILYSSRSNTMFEDVFINKFNLEKLVKMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYVLDQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGMYFVKKMIDSLGHEIEVCSKIGSYTIFNIYFYDISDY
                     LSIDS"
     gene            complement(18590..19270)
                     /locus_tag="PC545_000017"
     CDS             complement(18590..19270)
                     /locus_tag="PC545_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2454817.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEVVLDEFIDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIEILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(19422..21329)
                     /locus_tag="PC545_000018"
     CDS             complement(19422..21329)
                     /locus_tag="PC545_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2454818.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPVIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMKHIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSQVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMLSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(21330..22082)
                     /locus_tag="PC545_000019"
                     /pseudo
     CDS             complement(21330..22082)
                     /locus_tag="PC545_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(22398..23552)
                     /locus_tag="PC545_000020"
     CDS             complement(22398..23552)
                     /locus_tag="PC545_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2454819.1"
                     /translation="MINKAIKFKFIIFYFFSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDNINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTNKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYSSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSINIGYNYTLMVLSLFGCLAYCLIVLLNYLQE"
     gene            complement(23555..24022)
                     /locus_tag="PC545_000021"
     CDS             complement(23555..24022)
                     /locus_tag="PC545_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454820.1"
                     /translation="MFFKRKKEAITINSYIDGKIINLGEIPDKVFSTKVMGDGFAMIA
                     KDNILKSPTKGVISFIAQTNHAIGIKLQNDVQILIHIGLDSATTKKENFKLEVKQGQE
                     VNMGEKLVEIDNKLLENTLYIPLVIVENPNTVKYHLSNLNQEGNSGIKVINFD"
     gene            complement(24036..24524)
                     /locus_tag="PC545_000022"
     CDS             complement(24036..24524)
                     /locus_tag="PC545_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454821.1"
                     /translation="MITNKIKNITAVLKDENIFLSVVMVCFLILPVLYIVYLELTSLF
                     TNISFSQLLNNDVNASLNLISNFSAVYSAYVIYNIKKNKEIKENMIAFYLVLLGQIFQ
                     IYTITIVIMFTYIYKFVGIKQIISSIKELQWGKSYKALFSSIAVCLISLFVMIIRIKL
                     IM"
     gene            complement(24538..25869)
                     /locus_tag="PC545_000023"
     CDS             complement(24538..25869)
                     /locus_tag="PC545_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2454822.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVAKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGIAYGLRTIGPIIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(25883..27448)
                     /locus_tag="PC545_000024"
     CDS             complement(25883..27448)
                     /locus_tag="PC545_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2454823.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYIHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHIQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFGAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFVAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYVVQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDSEETKLYTKNEYLETKDEKGNKLSKASQQASE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(27618..28403)
                     /locus_tag="PC545_000025"
     CDS             complement(27618..28403)
                     /locus_tag="PC545_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2454824.1"
                     /translation="MRLEEMIFLNYNKLNDTDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(28570..29769)
                     /locus_tag="PC545_000026"
     CDS             complement(28570..29769)
                     /locus_tag="PC545_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HDF2454825.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLANKDEYTKRIKNTENDEEFIEEMSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKKVVDRYDSMNLKIKMPDDIFVKKELILKDVIKG
                     QIGYMYLPSMSSKNGSINNDLKIIGDYIDTLEKHKALIIDIRGNLGGSDSYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKQYTNARNIKLNPVENLPTSVKENAPKEIIKDFNDFE
                     DTSYTIKSTDDSKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKNGICDEEFKTIPEFKVKDCKRTADFKDDNCIRKAL
                     ELENINY"
     gene            complement(30291..31628)
                     /gene="xylA"
                     /locus_tag="PC545_000027"
     CDS             complement(30291..31628)
                     /gene="xylA"
                     /locus_tag="PC545_000027"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDF2454826.1"
                     /translation="MSEIFKGIGQIKFEGVKSDNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKVNMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKMNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(31724..33274)
                     /gene="xylB"
                     /locus_tag="PC545_000028"
     CDS             complement(31724..33274)
                     /gene="xylB"
                     /locus_tag="PC545_000028"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDF2454827.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDNEGKTLRNSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEITG
                     YVTRDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLGEDVYEIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDAIIKTSTKLEPISENVDTYNIYHRLYKRIYKSLKDDFKLLDE
                     VVNAKINN"
     gene            33482..34642
                     /locus_tag="PC545_000029"
     CDS             33482..34642
                     /locus_tag="PC545_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2454828.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSLSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHNSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKQLYYEDNHNAKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(34798..35202)
                     /locus_tag="PC545_000030"
     CDS             complement(34798..35202)
                     /locus_tag="PC545_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2454829.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTKELAAKLEKIGKDNISMVEQNASLLSDDE"
     gene            complement(35223..35705)
                     /locus_tag="PC545_000031"
     CDS             complement(35223..35705)
                     /locus_tag="PC545_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2454830.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKI
                     HNINVGNMSSRKDTTVLRPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(35725..36534)
                     /locus_tag="PC545_000032"
     CDS             complement(35725..36534)
                     /locus_tag="PC545_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2454831.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIVVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(36527..37306)
                     /locus_tag="PC545_000033"
     CDS             complement(36527..37306)
                     /locus_tag="PC545_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2454832.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQEQQYTVVGGMDEDE"
     gene            complement(37306..39384)
                     /locus_tag="PC545_000034"
     CDS             complement(37306..39384)
                     /locus_tag="PC545_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDF2454833.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     NFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEVECPLEEIPIFLKQDSSYNFKE
                     LKNILGEVKS"
     gene            complement(40183..40704)
                     /locus_tag="PC545_000035"
     CDS             complement(40183..40704)
                     /locus_tag="PC545_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2454834.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPHVRQFDEYLKNDYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCNELMDKINDIQMVDGKGIFIEELEKLLKEYNLN
                     IPIVGFDELREDK"
     gene            40870..41436
                     /locus_tag="PC545_000036"
     CDS             40870..41436
                     /locus_tag="PC545_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2454835.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTIGTVLGAVV
                     LGPFWSALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISKKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTSSSY"
     gene            complement(41653..43716)
                     /locus_tag="PC545_000037"
     CDS             complement(41653..43716)
                     /locus_tag="PC545_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HDF2454836.1"
                     /translation="MKAGEIEFLSYLEGSNKSFVVPVYQRNYNWKKEQCKRLFDDLED
                     IIDSGFRTHFLGSVVSIYSMGKEYLIIDGQQRVTTVSILLIAMCQIIKEFGESKGIIT
                     TEEQIKECYLINKHSRDKDGKIKLKPIAEDRKAYYELFEEEMNTDRNSNIVSNYLYFY
                     NRIKEDNISIDDLFDAIQKLTIVEIELKNGEDDPQLIFESLNSTGLDLSEADRVRNFV
                     LMGRSSKLQEEFYNNYWYKIEKNTSSNVSSFIRDYLTIKERSIPNKNKVYFSFKRYVS
                     DNGIDIEILLKDLLKFSNYYREISYSCTDDNDVNKVLKRINKLEIFVSYPFILELFDD
                     YNEGIINKKVLVESLNLVECFILRRIVCDVPPNALNKVFMNLGREIKKFSDYKENYSN
                     ILRYIFINKKSSQRFPTDSEFSNAFETKDIYNTKSKNKLYILERLENYNNKEIVDLEN
                     LINNNMLNIEHIMPQTLTNKWKESLGDNYKEIHDKYLHTIGNLTLTGYNSKLSNKTFE
                     EKKEMEDGFKDSRLYLNKYISSIDKWNEEEIKNRTKILLDRAVEIWKYPSVTYEPPSN
                     SENIFTLEDDDVNFTNESIVSFSILGESYKAKNWTEFYEQVALTLYDLDSVRFNSIVD
                     KTYKRDFIDNIKSRDAYKLRKGIKISENIYLETNLNTEAKLNIIRFLLNEFDIELNEV
                     IFSIK"
     gene            complement(44097..47432)
                     /locus_tag="PC545_000038"
     CDS             complement(44097..47432)
                     /locus_tag="PC545_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2454837.1"
                     /translation="MGNFDFLQKKKNFNSFNTACLEAEKSILVSPSTCATITRRALEL
                     AVKWLYANDSDLVLPYQDNLSTLIHNNSFIELIDYDMLPLLKYIVKLGNLSVHTSANI
                     ERGEAILSLNNLHQFVSWIDYCYSDEYTAEDFNEDLLLHGEEKRTRPDELKNLYERLS
                     SKDKKLEEVIKENEELRKSLTEKRKVNTVNYDFKIDEVSEFETRKRYINIELKLAGWE
                     FGKDIWEEIEVQGMPNESGVGYVDYVLYGENGKPLAVVEAKRTSKDPKIGQQQAKLYA
                     DCLEKRYSQRPVIFLTNGFDMYIWDDYSDRKVYGFYKKSELQLMIDRRKSKKSLSSVT
                     INDEISNRYYQKEAIRAVCEALENKQRKTLLVCATGTGKTRIAISIVDVLSRHNWIKN
                     ILFLADRKALVKQAKKSFTKLLPNLALCNLLDSKDSPEDARMIFSTYPTMMNAIDDTK
                     SKDGKRLFTPGHFDLIIIDESHRSIYKKYKSIFDYFDSYLMGLTATPKDEIDKNTYSV
                     FDMENGVPTYAYEYNKAVEDGYLVDYTSIEFKTKIMEDGIKYDELSDEEKEEYENTFN
                     DDESIGDEIGNNAVNEWLFNSDTIDLVLNKLMTEGLKIEGEEKIGKTIIFAKNTKHAR
                     AIVERFNKLYPKYGGNFVKAVDYSINYVDSIIDDFSDKNKLPQIAVSVDMLDTGIDIP
                     EILNLVFFKKVRSKTKFWQMIGRGTRLCPDLLGVDMDKERFLIFDFCNNFEFFKFNPK
                     GFEGNKTETLTEKLFNIKVSMVKELQDIKYIEGEYAELRKELLEELITSVKALNEDSY
                     IVRMNLSYVHKYKNENVWSNIGAVAQNEIREYISPLITSYSDDELAKRFDVVMYNIQL
                     AYLQNNNASKGIRHVMATAEKLSKLGTIPQIQQQKYTIEKAMTEDFWEDSDIFEVEEV
                     RISLRELIKYLEKSSQKIYYTSFEDMIVAEDRNDSVYNANNLKNYKKKVEYYLNSHKD
                     ELAIFKLRNNKKITKQDVETLEEILLKQLGNCDDYKKEFGDTPVSQLVRRLVGLDREA
                     ANEAFSEFLNNKSFNTKQIHFVKLIVDYVVKNGFIEDNKVLMEDPFRTVGSIIDLFEN
                     HIEERNKLIKTINKIKENASEIG"
     gene            complement(47482..47910)
                     /locus_tag="PC545_000039"
     CDS             complement(47482..47910)
                     /locus_tag="PC545_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454838.1"
                     /translation="MVIRNKLSWNSIEEVNRDFGSCLYDLQNFKILYDKEEMPELWAR
                     YIKEGFKSYMVSFLELTKAMLYYKSIDLNIKSKNFYDYLLACEYHNLLPKNSSIVIET
                     LRKLRNDDSHGYDIPQFEDMYELFTENEEVFVSIRNSCKK"
     gene            complement(47900..48280)
                     /locus_tag="PC545_000040"
     CDS             complement(47900..48280)
                     /locus_tag="PC545_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDF2454839.1"
                     /translation="MRNIPNEIKNSIINVFPKENTLAIIIFGSYGTDKHTSESDIDIA
                     WIPIKKVPITELATKTEALRNILDIDVDLKIVTDNYTIALKKAILEGEVIYKSKDFKD
                     YINKFYFENSDVIDILDWRDMYGN"
     gene            complement(48313..48621)
                     /locus_tag="PC545_000041"
     CDS             complement(48313..48621)
                     /locus_tag="PC545_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454840.1"
                     /translation="MNQIELDLYHNMVNIYKEADLQCNYKPTRFLQLINNKGALLAAK
                     ELINKEGATEGFTRLWECKRLDLSLEVLVLKEKYKELFTDEEREICINRLKDYGYKFN
                     "
     gene            complement(48666..49826)
                     /locus_tag="PC545_000042"
     CDS             complement(48666..49826)
                     /locus_tag="PC545_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2454841.1"
                     /translation="MEYRLNEVFEFIRNGASIKQFDTKFGYPITRIETIANSYIDTNK
                     MGYADIKELGKLEDYLLKNGDILMSHINSEKHLGKSAIYEDINKKIIHGMNLLCLRPD
                     KEVCYPKYLNYFFNSNEFRRQLPRIIKKSVNQASFSVNDLKNLKVDLPEMEAQKDISK
                     VLDKAQELIDKRKEQIEALDELVKSRFIEMFGNFKEQKFVLLKDCTNFIDYRGKTPIL
                     SENGIRIINAKSVGNGVFKYINEYISENTYNSWMKRGFPVAGDVLFVTEGHTFGNVCR
                     IPKDLKKFAMGQRIITIQGDKKILNNAFLAQYMQTKAFRVEIDKYKTGSSAQGIRSKE
                     LQKILIPVPSIELQNKFEEFVNQVDKLKVGIKTSLKELEDNFNSLMQKAFKG"
     gene            complement(49830..51293)
                     /locus_tag="PC545_000043"
     CDS             complement(49830..51293)
                     /locus_tag="PC545_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2454842.1"
                     /translation="MITGELRSKVDKIWETFWTGGLTNPLEVIEQFTYLLFIKGLDEV
                     ETKNEAEATLLGVSFEKIFDDEHQHLRWSKFSNEGNSEKMYEIVQNEVFPFIKKLHGN
                     KESAYAKYMGDAIFKIPTPLMLSKIVDGISNLELSKDRDTKGDLYEYLLSKVATAGTN
                     GQFRTPRHIIDMIVRLIKPTPEDIIVDPAAGSAGFLVSSQQYLRDNHSSLFLVQGLKE
                     HFNNGMFYGFDMDRTMLRIGAMNMMLHGVDNPNIEYKDSLSEVNTDKDKFTLVLANPP
                     FKGSLDYEAVSADLLKVTKTKKTELLFLALFLRVLKIGGRCACIVPDGVLFGSTGGHK
                     SIRKEIVDNHKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYS
                     LDDKRNEIKDSDIEDIIKRFDNLDGEVDRKRTEQSFFVDVEEIRENGYDLSINKYKEV
                     VYEEVVYDAPSVIIGRVRELEKEIVDGLEELERMLEV"
     gene            complement(51802..52671)
                     /gene="gatY"
                     /locus_tag="PC545_000044"
     CDS             complement(51802..52671)
                     /gene="gatY"
                     /locus_tag="PC545_000044"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2454843.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRVGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDDLDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIEIVKC"
     gene            complement(52691..54607)
                     /locus_tag="PC545_000045"
     CDS             complement(52691..54607)
                     /locus_tag="PC545_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HDF2454844.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKANELIQRCIDG
                     KVAIRKGNLSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNVTKDEHKNESAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMIGILDKSSVLLDKKKFKEEILKREEISSTGFGMGIAIPHAKTDAVKVPRVAVGL
                     SKEGFDFESEDGSLAHIIFMIAATDNGDNLHLKTLSQLSSKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(54610..55530)
                     /locus_tag="PC545_000046"
     CDS             complement(54610..55530)
                     /locus_tag="PC545_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HDF2454845.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFDVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLDKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICENKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGKSIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(55632..56384)
                     /locus_tag="PC545_000047"
     CDS             complement(55632..56384)
                     /locus_tag="PC545_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2454846.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTVNKSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
     gene            complement(56776..58221)
                     /locus_tag="PC545_000048"
     CDS             complement(56776..58221)
                     /locus_tag="PC545_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2454847.1"
                     /translation="MIKLGKFPKGFLWGGAVAANQLEGAWNEGGKGWCLADVHMYDKD
                     RDISKPYESDMTIERIKFAMKDNNGYYPKRSGIDFYHTYKEDLKLLKELGLKCFRFSI
                     NWARIFPNGDDLEPNEEGLKFYDNLINEVIDNGMEPIVTLLHYETPLNLVLKYKGWSN
                     RKVVDFFVRYAELILNRYKNKVKYWIVINQINLIHYESFNSVAFCVDQVDNVEETRYQ
                     AIHHQFIATALTKKLGKEINSDMMIGMMMADCTSYPEDCDPENVVFALKRNRLQYFFT
                     DVSFRGEYPVYIKRFFKENNINIVMEEGDDKILKENTMDFLALSYYYSTTVSASKNTM
                     NPTDNIKNPYLKANPWGWAIDPKGLYNTLSQYYDRYQKPIMIAENGFGMHDKFEDGKV
                     HDSYRIDYLGKHIEQIGEAIADGTEILAYCMWSPIDIVSCSSQEMEKRYGFIYVDIDN
                     NGNGSKKRYKKDSFYWYQNVIRTNGEELKEK"
     gene            complement(58243..58596)
                     /locus_tag="PC545_000049"
     CDS             complement(58243..58596)
                     /locus_tag="PC545_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2454848.1"
                     /translation="MTIEEHIIDEELVEVAMQIILRAGEARTEIKHALNDLEKFDYRN
                     ADLKLAKAKGFMTEAHRAQTKIIQGEAGGEKRAHSLLFAHAQDTLMTIFSELNITMSL
                     VGIVKSIEYKVNNSK"
     gene            complement(58625..59872)
                     /locus_tag="PC545_000050"
     CDS             complement(58625..59872)
                     /locus_tag="PC545_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2454849.1"
                     /translation="MGRVFMNKFIDFLSEKFTPVVNNMTKNIWVQSVQSTIMKVLPMV
                     FVGSLVTIVSVLKNYISFLPDLSPINQYTFGLLGLFIAFLLPMEIMKNKKFESMSVVA
                     GLAGAGLMLMMIRPEITNEGAIFNFNRFGGEGMLVSLVAGLFSGLIMSLFGSFSFFGE
                     DSALPDFVSKWFDNMLPITVIMFVGWGLIYGLNFDMFEIIVRVFKPVSDIAQTLPGFV
                     LLNFLVVFIYSLGISGWVLSPITYSIQLGAIAANAAAVASGGVATNIYVYEVIQTGWI
                     TLGGLGATLPLVIMMCLSRVGRIKAIGRACIVPSIMNINEPVIFGAPVAWNPFLMLPM
                     WIGTVVISITTYLSMSMGFVTIPSKVFNLWYVPFGISTFTVNQDVRGLIVLAINIVLL
                     FMIWYPFFKAYEKEEIKLESQEV"
     gene            complement(59880..60197)
                     /locus_tag="PC545_000051"
     CDS             complement(59880..60197)
                     /locus_tag="PC545_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2454850.1"
                     /translation="MKNIRVLLACGSGASTGFMATSMRKSAKKKGIEADIKARSESEI
                     DKYLSEIDCLLLGPHLDYMKEEIQQKANEYNVPVACISKTAYGRLDGAKALEEALELI
                     SNK"
     gene            60439..62352
                     /locus_tag="PC545_000052"
     CDS             60439..62352
                     /locus_tag="PC545_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2454851.1"
                     /translation="MKTSKKELFLNFIKTQHDWIDSSTLANYLNVSTRSIRKYVNEIN
                     SNGEFILSSKKGYKVNLNNNCQTKVDSSENISPDNRLNLILKELIVNSNGINIFDLSE
                     DLFVSPATIEGDIVKANKFIGSYNLKIKQSKFLLKLIGNESAKRKLMSSIIFKETGSD
                     FLSLFDVQKIYQEYNLTKLKENIIYILKKYNLFINEYAINNILLHLMITIDRIKKNNY
                     IESVEVVNYIDNNNDISISRDISNFLESEYNITLTSAELYYLVFQLTNKTTVLNYHEM
                     DTKSLPNYIDEHFVKLTKKIIKNVYDLYFIDLSDDEFVVKFTLHVKNLISRAKNNQVL
                     RNQIPQKLKDSYPLIYDISVYICNQIQILENIEIDEDEISYISLHVGSFFDRQKLLED
                     KILCALITPNYYDLQFKIVRDLEKRFNESIEIIQIFSDTHNLDFDNKVDMVITTLPIS
                     DRCPIPFVYVNPYLNRKDYDNIQSKFIQIKDRKNILTVQNHLEMYFSESLFMKNIYLD
                     SAKDYIRFMGNILYENKYVKPNYIDDVLIREKMSSTAFNNNVAIPHSMKMDALKTGVC
                     LIVNDKPVKWGEEKVQIIAMIPINEKEKEKFNYIFESFIEILSEWNNVKELTKADNYS
                     SFMNRIAYLIQNI"
     gene            complement(62475..63629)
                     /locus_tag="PC545_000053"
     CDS             complement(62475..63629)
                     /locus_tag="PC545_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDF2454852.1"
                     /translation="MKILISIDSLKGSLSSIEAGNAIKKGILKVKEDAQVKILPLADG
                     GEGTVDALVQGMNGKKDTIEVTGPIAKKVDATYGLLKNTSTAIIEIAQASGLTLVPTE
                     LRNPLYTTTYGVGEIIKEAINKGYRNFIVGIGGSATNDAGIGMLQALGFEFYDENNKL
                     VGLGGKVLNEIRHIKIENRLKELDECKFKIACDVNNPLFGKNGAAYIYGSQKGATSEI
                     IEELDNGLRNFSKVVKNYLSKDVANVEGAGAAGGLGFAFLAFLNSKLESGIKIILEEI
                     KLEEELKDADFVITGEGRLDNQTAMGKAPIGVAKLAKKYGVKVIGLAGATTEDAVKCN
                     EEGIDAYFSIVNRAMTIEEAMDKATASENMTATTTQIFNLITSIQQSKNM"
     gene            complement(63889..66503)
                     /gene="mngB"
                     /locus_tag="PC545_000054"
                     /pseudo
     CDS             complement(63889..66503)
                     /gene="mngB"
                     /locus_tag="PC545_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728444.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
     gene            complement(66647..68572)
                     /gene="mngA"
                     /locus_tag="PC545_000055"
     CDS             complement(66647..68572)
                     /gene="mngA"
                     /locus_tag="PC545_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDF2454853.1"
                     /translation="MEFKKITNENLVFLKQKIKTKKDAIKFLARKLYDEGKLTSYEEY
                     LEAVMYRETLSPTGFERGLAIPHGKSKSVKEAAFAAMTLETPIENWESVDPNNKVEII
                     FLIAVPENEGGNTHIQILSELVTRLSNEDYMNMLLNSKTKKELFNNLDIKEKKEQIKE
                     EIRIQNESKKIILAITACPAGIAHTYMSAEALIKAGKEIGVDVYVEKQGANGMVDPHT
                     PDIIKRADAIIYATDVAPKNTERFEHLPNIKTSVAAPLKRAKEIIYEALEVAQKQGKG
                     DYIEKSSDISYCEKSSWKKDVKSAILTGVSHIIPLIVAGGMILAFAVLASQAFGLQEL
                     YNQEGSWLWQLRKLSGGMLGTLMVPVLSAYMSYSLAEKPGLGPGFAAGLAADLIGSGF
                     LGGMLGGLLAGYIVKFLKKNIPAKGTLAGFVSFWVYPVVGSLVVGLLMLFVVGKPVAA
                     LNNGLINWLNGMTGVNAILLGAIIGAMVSFDLGGPINKAAYTFAIGAIASGNFIPYAI
                     FASVKMVSAFGVTIATVLGRDLFSESEKEIGKQTWILGLAGITEGAIPFMIEDPIRVI
                     PSLVAGSAITGSIVAFFNIGLQVPGAGIFSLALLEGSMSGFIIASIWFFAAVLGAIIS
                     AVLLIITRKQKNQRKIH"
     gene            complement(68702..69427)
                     /locus_tag="PC545_000056"
     CDS             complement(68702..69427)
                     /locus_tag="PC545_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2454854.1"
                     /translation="MEFYERAKKYEYKFTDTEDMIIEYMRKNKEEIVKLSIQSLADKF
                     FTVPNTIVRLCKKMEYDGFSQLKNNLKEELKNEDKEPKNGFESDIKKTLELIDYEKIN
                     KVADIIQKSRSIIVYGIGQNIPLCEILVKEIRGGHDNITYYSQRHEVLHDIGKLGEQD
                     MFFALSLSGETKEVIEVAKTAVNKGCKVVALTHICENQLQQIADISLYCYSTIKQVNG
                     YNISDKVPIMMIMRAIIEQYWLR"
     gene            complement(69690..70460)
                     /locus_tag="PC545_000057"
     CDS             complement(69690..70460)
                     /locus_tag="PC545_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HDF2454855.1"
                     /translation="MKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPFSRQ
                     AIEMVKNDDVCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLTGKEVF
                     NYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEDVSISAMCDAAKEYGIPSCPYYLD
                     SCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSSLIQ
                     ERVYEHAMLCDKEVKMWLKRNNIELVDFKDIFKSNNYI"
     gene            complement(70462..71889)
                     /locus_tag="PC545_000058"
     CDS             complement(70462..71889)
                     /locus_tag="PC545_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2454856.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNISN
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLSPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTSTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKKKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGVRLGSFNGASIIDYVLSFSYGDKAW
                     LVIPVGVVFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEMQLDELRKQADA"
     gene            72280..73002
                     /locus_tag="PC545_000059"
     CDS             72280..73002
                     /locus_tag="PC545_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2454857.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNTEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKVGSEVYYLHRLRYIDNIPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            73199..74866
                     /locus_tag="PC545_000060"
     CDS             73199..74866
                     /locus_tag="PC545_000060"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDF2454858.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLYYLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEKLLKEAHKRDIKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDETTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGAKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALLILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSRSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNLLVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDIIENKMEIKPYESIMIYYK"
     gene            complement(74962..76281)
                     /locus_tag="PC545_000061"
     CDS             complement(74962..76281)
                     /locus_tag="PC545_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2454859.1"
                     /translation="MEKGIKLGFWSIVLLGINAIIGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGDFVGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLIKNIIIGILVVGLGIINFMGMKQSKHLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSASSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLAGSATPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRQTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYSKKYNDVEVK"
     gene            complement(76322..77068)
                     /locus_tag="PC545_000062"
     CDS             complement(76322..77068)
                     /locus_tag="PC545_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2454860.1"
                     /translation="MKKLAKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CKAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDVNPLIWKEEPHNKLGAISPKRDSF
                     DMKLLKHALDMKKPVLGICRGEQIINVTEGGSLYQDLSLIEGAYIKHNQQHLSNVPTH
                     TVQIKEGTKLYEILGEKEVLVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTRDYDNMKKIFMAIVKEASK"
     gene            complement(77312..79321)
                     /locus_tag="PC545_000063"
     CDS             complement(77312..79321)
                     /locus_tag="PC545_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2454861.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGESSLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGMAMVSDKGNIIICNNSLRDILDIQSNIIGKNIDELENENIKKIFSLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSTKMERTKKLGMKIAESDYTILITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKGDRFRADLYYRINVLPINIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     NDLPHNLLNITEDFSKEISILKDRTSIEKVKLVMECLKNANNLNKNIGRNGIVNSINE
                     LGYNITEGEVRGILNMLKELDLIICESGRKGSELNRRGKNILNSI"
     gene            complement(79715..81148)
                     /locus_tag="PC545_000064"
     CDS             complement(79715..81148)
                     /locus_tag="PC545_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2454862.1"
                     /translation="MALRKDFLWGGATAANQCEGAWNLDGKGPSCSDMCTGGSQKVSK
                     RITRVIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEIPFAITEKYNGWVSRDVIDLYMNYCETIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFINQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPDDAVKTQRDSRVMNYFCGDVQVRG
                     EYPTYMNRYFGENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSDSKEDNTSGNI
                     LGGVKNPYLKSSEWGWQIDPEGLRYALNEIYDRYRIPMMVVENGLGAYDKKESNGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFDWYKQVIESNGERL"
     gene            complement(81235..83151)
                     /locus_tag="PC545_000065"
     CDS             complement(81235..83151)
                     /locus_tag="PC545_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454863.1"
                     /translation="MRKYEQLSKEIIANVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSAGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSDSDAPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVLLAAKLEKVFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGVFVPSFAQTAVVAAMYFKLKDKKIKELCIPAVISGICGVTEPAIYGITL
                     PRKTPFIYSCVGGAVAGAVMGIMNVKSYVMGGLGIFGIPNYINPTTGDMKGVYATIIA
                     IIVAIVVGFVLTLFFWKDDAIVEISKDDNGQGVIEKNKREVVYSPIKGEVKALEQIQD
                     AAFSTGILGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLITFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHVNNSQKLITVLA"
     gene            complement(83225..84085)
                     /locus_tag="PC545_000066"
     CDS             complement(83225..84085)
                     /locus_tag="PC545_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2454864.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGEHISKDKI
                     DKIFKLSDPNISDKFKELIADIPIRYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVSVKNVLLWDIKRFYKSEFKIGLEALDYIEKKFEIRLSEDEAGFIALHI
                     VNAQMDQSIKTIYEITQIIQEISNMVKYHYRIVFDEDSVYYHRFITHLKYFAERIVSN
                     NLHENNEDDLLNVIKVKYKNAYKFIEKLDEFIHKKYNYDLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(84436..85623)
                     /locus_tag="PC545_000067"
     CDS             complement(84436..85623)
                     /locus_tag="PC545_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2454865.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMKEYGFDTVYPYCNMETAFRAELGDNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDISPMVYVHEKEFLNYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(85637..87028)
                     /locus_tag="PC545_000068"
     CDS             complement(85637..87028)
                     /locus_tag="PC545_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDF2454866.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKKFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQIAPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDTNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFVIPLMVVLTIL
                     VMAFLTIGLYLGF"
     gene            complement(87623..89323)
                     /locus_tag="PC545_000069"
     CDS             complement(87623..89323)
                     /locus_tag="PC545_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2454867.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVSALGLSGLIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NANKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFVAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYRERQSVDAGKTAYNNAVEEEVDDKDTKLVHSIIEGLGGSNNIKNVTNC
                     ATRLRVELNSIDGFYEDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(89295..90698)
                     /locus_tag="PC545_000070"
     CDS             complement(89295..90698)
                     /locus_tag="PC545_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2454868.1"
                     /translation="MIVNKELIIDYLKSNQENIINDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIQIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNDKKDLVESLAYYTEIEELEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLEKIKKLNDKGVIIRDILNSYFTDDNHGKKLGIYKEDVDMGVSTICIMS
                     IFLEKNELNMKIDFRYPKGISWEFITNRINEIGKKENLIVDIYKDLKLLYVKPDSELI
                     NKLSNAYKQGFGKEAELFTKGAASYARVLKNGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
     gene            complement(90962..91453)
                     /locus_tag="PC545_000071"
     CDS             complement(90962..91453)
                     /locus_tag="PC545_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454869.1"
                     /translation="MFNIFKKKENKIEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVNVFDTKHAIMIKSNDNTNILIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
     gene            complement(91479..92291)
                     /locus_tag="PC545_000072"
     CDS             complement(91479..92291)
                     /locus_tag="PC545_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2454870.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEVMEKGIKVALVTG
                     RPYNAMKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLSKH
                     FKGCKIVISNNEIGEEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVDSLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIKFAGLGIAMGNATDEVKSMADFVTDTNVNDGVAKALRKILS"
     gene            complement(92304..93455)
                     /locus_tag="PC545_000073"
     CDS             complement(92304..93455)
                     /locus_tag="PC545_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2454871.1"
                     /translation="MINFYQPSRINFGQGTLSELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLNLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNIEKFAKVARMLNQELEKVEDDEAAKTLGNEIES
                     FLKRVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            93725..94366
                     /locus_tag="PC545_000074"
     CDS             93725..94366
                     /locus_tag="PC545_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDF2454872.1"
                     /translation="MKYLIDSANLDEIQKCLKYGFKGITANPSMYLKEKVNFHEFIET
                     CANLNPEILTAEVIGNNLYELINSCDKILNINKNTIIKINFSEVGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICNLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTIDGANTFERDWSKVNK"
     gene            94392..95936
                     /locus_tag="PC545_000075"
     CDS             94392..95936
                     /locus_tag="PC545_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2454873.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEELKVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLHTLQVEK
                     SNLSDEYYINFLIHIAICIKRISIKRYINLSEGFFRELLEHEYFEKSKILCANLSEAF
                     DVEFIDEEVCYILLHIEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGSILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(96136..96762)
                     /locus_tag="PC545_000076"
     CDS             complement(96136..96762)
                     /locus_tag="PC545_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2454874.1"
                     /translation="MKKEMIKDYLLITISAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPLFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINNACAQ"
     gene            complement(96897..97613)
                     /locus_tag="PC545_000077"
     CDS             complement(96897..97613)
                     /locus_tag="PC545_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HDF2454875.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTIGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIT
                     AGVKNCRKAIDKAATKFGLE"
     gene            98039..99253
                     /locus_tag="PC545_000078"
     CDS             98039..99253
                     /locus_tag="PC545_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2454876.1"
                     /translation="MRNCIIAQSGGPTAVINASAVGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYKINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFIVETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASDTDLKQAAEVGAYGIELSKKGYTGVMAII
                     NRTCNNPYKYEVSHTDISKIANKTKYFPKEWINEECNYITDEALNYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            99873..101201
                     /locus_tag="PC545_000079"
     CDS             99873..101201
                     /locus_tag="PC545_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454877.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDRFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQNAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTACYIKESIENSTNIKVCVGYGIGNDIKQAMSSAISANKESKINHLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(101329..102519)
                     /locus_tag="PC545_000080"
     CDS             complement(101329..102519)
                     /locus_tag="PC545_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2454878.1"
                     /translation="MKNFFKEAQSIEGEIIRWRRYLHENPEIGCNLPKTVTFVTSTLE
                     KMGYSPKLTSNNGIVAVLDSKNGGKTLLLRADMDALPIMEETDLPFKSKNKDASHSCG
                     HDNHTAMLLGAAKILMDNKHLLKGRVKFVFEPDEETTRGAKSMIDDGILENPQVDAAM
                     AFHSMVGKFLNTGQVAYSRGPAMASADIFEITVEGKATHGASPEFGVDPINIMSHIQI
                     ALNTILSREKPQKEPVVLTIGMFNAGSVPNAIPNKASMSGTLRTFDQSVREKLKNRII
                     EISTGIAKSFGGNAKVEFKMGTPAVINDLDVGDEIVSYVKEVIGNENVIDFPSAMGSE
                     DFSEILLRVPGVFLWIGMGSISQGYNFGMHNPKAIFNEEGLVYGSALFAHCAVKWLEN
                     NFSR"
     gene            complement(102625..104004)
                     /locus_tag="PC545_000081"
     CDS             complement(102625..104004)
                     /locus_tag="PC545_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2454879.1"
                     /translation="MIGSISINEIDKKRFKLDDLSKKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEVGVGGLATAIRASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGLKREMIENNLSGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYTRALSREAVESTYERLVKVAKG
                     AAMMTETEVEVEFLGGCYNTLNNHVLANLVSECMNEIKQEPWTREELDFAAELDRQSP
                     QQYKAMISKYNLPEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYASKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(104035..105243)
                     /locus_tag="PC545_000082"
     CDS             complement(104035..105243)
                     /locus_tag="PC545_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454880.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLL
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALVGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPSMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIIPTIGILVVGSVFIILFTAIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            complement(106268..107722)
                     /locus_tag="PC545_000083"
     CDS             complement(106268..107722)
                     /locus_tag="PC545_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2454881.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYEGLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     RRFDDKVKYWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKIGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNITIEVTDEDKKAFKEGKLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPIGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDDY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKSNGEVLDFSTDIVVG"
     gene            complement(107742..109613)
                     /locus_tag="PC545_000084"
     CDS             complement(107742..109613)
                     /locus_tag="PC545_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454882.1"
                     /translation="MKYKDLNENIIKLIGGKENIKSVAHCVTRLRFTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSIKSERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILQSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWAMGIIIEGLKKILPKALHYFLVPVLSLIITLSVTLTVFGPIGMWIGDGLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPISLNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGI
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEVSNFTFMIISVVVSFVAATAASYLLGIP
                     SDEKEEVKGDNEKELELIINENNSRTISIGNVAKGQVIALGEVNDKAFSSGALGKGVG
                     VIPSESKSTVISPVDGTITIVFPTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFESDKIIEEGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            complement(109603..110463)
                     /locus_tag="PC545_000085"
     CDS             complement(109603..110463)
                     /locus_tag="PC545_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2454883.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKALNIKASDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMHALKLIGDSKGINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVELDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            complement(111058..111462)
                     /locus_tag="PC545_000086"
     CDS             complement(111058..111462)
                     /locus_tag="PC545_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HDF2454884.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNCIKPWKDAETYIDKYMVK
                     QDISKDNIKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLRSNEELKSVKYPLIYLKYKPDK"
     gene            complement(111654..112841)
                     /locus_tag="PC545_000087"
     CDS             complement(111654..112841)
                     /locus_tag="PC545_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDF2454885.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVDRGNEVLYYSFNEFKDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPGLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQNFIEIIRMIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCISAFKDSDVDVIMSVGRSTDIKSLGNIPNNF
                     EVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVAKRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(112873..113298)
                     /locus_tag="PC545_000088"
     CDS             complement(112873..113298)
                     /locus_tag="PC545_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2454886.1"
                     /translation="MINENLKSLRKINQYTQEELAEKLNVSRQSIAKWESGESIPDIC
                     SCIKLAKLYNVKLDDLVNHSEEKTGIIVPPKGKFFFGAVVVGERGQIVIPKEAREVFN
                     INAGDKLLVLGDEERGLGIVHQRDLINFIGHVGVVRKEE"
     gene            113756..114505
                     /locus_tag="PC545_000089"
     CDS             113756..114505
                     /locus_tag="PC545_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2454887.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKETKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGTIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(114671..115036)
                     /locus_tag="PC545_000090"
     CDS             complement(114671..115036)
                     /locus_tag="PC545_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2454888.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILIALLESDFN
                     GIRVGEITKKTHLSRPAVSHHLKILKEAEIVNLRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(115052..115828)
                     /locus_tag="PC545_000091"
     CDS             complement(115052..115828)
                     /locus_tag="PC545_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2454889.1"
                     /translation="MKYQHIIEVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPTKAITLTGFDEPPIELTNKPKLDSDELLMAIKSRRSIRKFKDKEVSSE
                     IIKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDNFFFKHAPIAIMIITKDKISGSLAASNMALMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEVATVRYL"
     gene            complement(116275..117702)
                     /gene="ascB"
                     /locus_tag="PC545_000092"
     CDS             complement(116275..117702)
                     /gene="ascB"
                     /locus_tag="PC545_000092"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2454890.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDEHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPKGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEVDSNNLIGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGITPEMEPGDIELLNKYTVDFISFSYYNSRCVRTDENADDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVSIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRMKKKSFEWYKKVIASNGEDLY"
     gene            complement(117763..119691)
                     /locus_tag="PC545_000093"
     CDS             complement(117763..119691)
                     /locus_tag="PC545_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454891.1"
                     /translation="MGKYKQLAQEIVNNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDDSSTSSETKKSGNILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKCVPNLVKFFFVPMLTLLITVPVALIV
                     IGPIATFGSTLISQIVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVTSPIKGEVLKLS
                     DIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDTGVEVLIHIG
                     LNTIQLEGRGFEACIKQGDRITKGQTILNFDVYAIKELGYSTVTPIVITNSSQFLDVV
                     ETESKNIELEDNLITVLF"
     gene            complement(119836..120678)
                     /locus_tag="PC545_000094"
     CDS             complement(119836..120678)
                     /locus_tag="PC545_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2454892.1"
                     /translation="MIINKILNNNVVITLDDNDEEVIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEISNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGIKLPEDEAGFIAFHI
                     VNAQLSEGHTLASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLD
                     SAHEGETDKELLSIIKSKYNKEFECVVKIKNFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNM"
     gene            complement(121025..122395)
                     /locus_tag="PC545_000095"
     CDS             complement(121025..122395)
                     /locus_tag="PC545_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2454893.1"
                     /translation="MKNKYEEVAQLIIDGLGGKDNIIDLTHCMTRLRFILKDEKKVNA
                     DKLKSIDKVVGVNSTSTQYQVIIGNEVGVVFNAIINKGINTTGDIKSDIKNKNKKNSI
                     LNNIIDTITGCMTPMIPALTASGMIKVILSLCTTFNWLSSNSSTYRVLDIIGDVAFYF
                     MPIILAINAAKKFNVNTSIAVIVVGVFLHPNFSAWVSSGDPISFIGVPIQDVIYAASV
                     IPALLTVWMMSYIEKFIDKLTPSMLKTILNPTLVLLISAPIALIVIGPIGNFLGEGLA
                     SIINLLQGRLGFIMVCLLAAAMPFIVMTGMHHALTPIFISAFATTGQESLILISQVCA
                     NLAQGGATLAVALRSKDSKMKQLASAAWISAIMGITEPALYGVTLKLKKPAMLASIGA
                     GIAGLFAGITHVTLYVPQNSIMAVLGLSGEKGISNIVNGLIVIFIALVAPFIMTYVFG
                     VEKNVK"
     gene            complement(122434..123105)
                     /gene="pgmB"
                     /locus_tag="PC545_000096"
     CDS             complement(122434..123105)
                     /gene="pgmB"
                     /locus_tag="PC545_000096"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2454894.1"
                     /translation="MKAVLFDLDGVITDTAKYHFEAWHEIAKSLDIEIDETFNEKLKG
                     ISREESLDKILLSANKVDSYTKEEKLKVCEEKNNIYIKLINSLTYKDILPGINELIKE
                     LKKNNVKLGIASASKNAPRILESLGIKDEFDCIVNPSLLKRGKPYPDIFIEGSKILNL
                     KQYDCVGIEDSYSGIKAINAANMTSIGVGKKEVLGKCDHIVEDTSLLNYNTLLQAWSK
                     KINLD"
     gene            complement(123107..125497)
                     /locus_tag="PC545_000097"
     CDS             complement(123107..125497)
                     /locus_tag="PC545_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2454895.1"
                     /translation="MSLIFTTKDQLIEQGFSKDTIKSNETLFTLANGHLGVRGNIEES
                     DFNNEYVDNMGTYVNGFYEESPITYGESAYGYAKQNQTICKLPNAHIVNFSIEGDCFD
                     LSKGITSEHSRILDLNEGILKRSFIWENSRGRKVKVSIERLVSQDIEELMMISYTITP
                     LNFNGKIKFKNKMENSMEKFNCEKVEDPRVASINKKQFKINVKKYNNKYFMNLVTERS
                     RLGLWCGFDSYITCGNITNETYNVSDECIEREIEVYAKKDERLNLEIVVGYSRVYSDL
                     NLNEERLKSLECLESLKCLESEAHLKSLELVLREACNIGFDKVKNIQRKKMNEFWTTS
                     DVEIEGDKTLQLGIKTNLFHIYQSVGRDGKTNISAKGLTGDGYEGHYFWDTEMYILPF
                     FIYTQPSIARALLDYRYSILDASRERARELGINKGCLYSWRTINGNECSAYYPAGTAQ
                     FHINADIAYGIRTYFEATNDEEFMMQNGLEILIETARFWVEFGDYIESKNNKFCINGV
                     TGPDEYTAIVNNNYYTNLMAKYNLIYAVQMVDKFKHNLFSNEVFRKVKLQEYEVKNWI
                     NAAQNMYLPYDEEKKISKQDDSFLEKSVWDFKNTPKEKYPLLLHYHPLTIYRYQVNKQ
                     ADTVLSALLFPDEFSLEQKKRDFEYYEKITTHDSSLSRSIFGMMASEIGNHEKAYNYF
                     MDTALMDINNLQKNTSDGIHAANMGGTWMSLVYGFAGMKIKNDKLSFEPRIPKHWKNI
                     KFNILFRDNLVSVNIEEYKSYYKLIKGRNITILHDNKEIKLSKNIIEVNNCFKGSN"
     gene            complement(125523..126971)
                     /locus_tag="PC545_000098"
     CDS             complement(125523..126971)
                     /locus_tag="PC545_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2454896.1"
                     /translation="MKLGKDFLWGGAISANQSEGFYLDGGKGLSQFDKLPLNDKRLKD
                     VCLDEDNILNENNQYYPSHIGIKFCENYKEDIAMLAEIGFKCFRFSISWSRIFPTGEE
                     SEPNEKGLQLYDNIIKELKKYNIEPLITISHFDIPYSLVEKYSGWADRRVVNLYVKYA
                     EILMKRYKDDVKYWIPFNEMNMIMHIPYIGAGLTFIADENQLQKKYQAAHHQLLANAK
                     TIEIGRYINKEFKFGCMLAAGKTYPYTCKPEDVFATLEHERHNLFFSDVQVRGVYPSF
                     MNYYLKSNQIKLIIKEEDLSTLKDNTVDFVSFSYYSSACSSARAEGLEKSKANGPETI
                     KNPYLPKSNSVWQNDPVGLRITMNQLYERYQLPLFIVENGLGTQDIVNEDGKIIDDYR
                     IDYMKEHIKNMIIGVNEDGIELIGYLMWGCIDVTSVSEGKMTKRYGIIHVDSDDYGRG
                     SFKRTKKQSAFWYKKVIETDGEILFNDMETHA"
     gene            complement(127374..128261)
                     /locus_tag="PC545_000099"
     CDS             complement(127374..128261)
                     /locus_tag="PC545_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2454897.1"
                     /translation="MAIYLEMPKLIHEFPFRSLSNEGEVLTTPHWHKEIEILLITEGV
                     VNLFINDKPMQLKKGEIVIIKGGDIHYILPSPGSERLVFQFDISFFNDLILLNEEKES
                     LINLFSSIKKCSRDWSAKIRKSVFDILIDIYNEDLYKIEGYSYAIKGKMYNLIPILYR
                     QIPREIEENTVEYEINSKEILERLNNIFKYVEKNYKQPIKLEDVSYEVGFSVYYFTKF
                     FKKNTGKTFITFLNEYRLEKAKWILLNNNISIVDLVEEVGFGSTKTFYRVFKEKIGVS
                     PLEFKKRYNSLEKKGSVSK"
     gene            complement(128361..128810)
                     /locus_tag="PC545_000100"
     CDS             complement(128361..128810)
                     /locus_tag="PC545_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2454898.1"
                     /translation="MEIREILTEESIILDLKSITKEGIIQELVDKLYDTGSITLKSKF
                     IKALKHREKIGSTSIGYQMAMPHGKTKYVKKASIVIGKSKKGVDWPSIDGLPVKVIIM
                     FAMPQSGGNEDMILISKIAYILGGKNIGERLLNANTKKEILDIFYEV"
     gene            complement(128800..130707)
                     /locus_tag="PC545_000101"
     CDS             complement(128800..130707)
                     /locus_tag="PC545_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2454899.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKMIE
                     KYLETFNIKIDIKRGYGILIEDLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISELTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTINQPYYINLITHILICLKRIEEGNQIESQEEREISVDNLDELVYKNVSTLIDKIEN
                     RYRVKITKDEIMYIYIFLVSSGFSSENNSSYNDESDRLSESEKVSSIMIENMSRFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSKRNLDDVDFVISTVNLDLKDKLHIVVSVLLMENDINNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVAQMESLIGVNLGSKYESISLGKDLNIYIHKQSKLN
                     KGIMNINNVTRTIDIYLEISNQSFLKNILMEISNLYRQKNYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(130975..132870)
                     /locus_tag="PC545_000102"
     CDS             complement(130975..132870)
                     /locus_tag="PC545_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2454900.1"
                     /translation="MIGKILRSDCIVLDKQLQTKKDVINYMANHLYHGNYINNKEKFI
                     ADINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDREITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMEENFLKHLLSAETPYEILSIFNNKAAESGDFSNNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQLSRFIGKKVLQKKVVDAKNVSLAKNLIINAENGMAKEIKPGGKTLEDSK
                     EMLQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFATG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSAPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVELLGA"
     gene            complement(132906..133784)
                     /locus_tag="PC545_000103"
     CDS             complement(132906..133784)
                     /locus_tag="PC545_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDF2454901.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREIVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FVEKTGVETLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCRELLEQNPSLYEPDTIYPPCIKSAREVIEFKMN
                     LFNAIDKLKCFYKNKN"
     gene            complement(134489..135922)
                     /locus_tag="PC545_000104"
     CDS             complement(134489..135922)
                     /locus_tag="PC545_000104"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2454902.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KITDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPKGDESTPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     NRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAMPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITEEDKKILKEGIVDFIGCSYYLTTVVTADKTMKTTGNDSAGKA
                     DVVENPYLKISDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     DYRIEYLASHIREMKNAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(135945..137786)
                     /locus_tag="PC545_000105"
     CDS             complement(135945..137786)
                     /locus_tag="PC545_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2454903.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETIIKKAVLQGQNTEEQGEKVSIVSKIFE
                     SISGAFSPLIPALAGAGMIKALLTILTTMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLSANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDIISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     NGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYTTHMNAGDKVKKGDRIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(137912..138745)
                     /locus_tag="PC545_000106"
     CDS             complement(137912..138745)
                     /locus_tag="PC545_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2454904.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGREELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYNFSKKAVEYLRKKLDKNIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENTNVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(139789..140001)
                     /locus_tag="PC545_000107"
     CDS             complement(139789..140001)
                     /locus_tag="PC545_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2454905.1"
                     /translation="MAILMKTKIKEYREKLLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEEE"
     gene            complement(140002..140418)
                     /locus_tag="PC545_000108"
     CDS             complement(140002..140418)
                     /locus_tag="PC545_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454906.1"
                     /translation="MSKSETGVRSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNLDTGIILIAVLFIQSLSKLVLYIYFDKRGICN"
     gene            complement(140488..140901)
                     /locus_tag="PC545_000109"
     CDS             complement(140488..140901)
                     /locus_tag="PC545_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454907.1"
                     /translation="MSKSELGVKANLVSELMMLSYFFIDLLLQKFIFKGEGIWFTFII
                     LLAFWVCNLMYNKKYNDLVDEASKLMLLKVDQIASKIMLYSMFVIAVLFSVNHTRALD
                     ISNLDIGIMLLAVLFIQSLSKLILYIYFDKKGICN"
     gene            complement(141735..142364)
                     /locus_tag="PC545_000110"
     CDS             complement(141735..142364)
                     /locus_tag="PC545_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDF2454908.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKVKEALIQ
                     DSIVAAICGATLGLAKKGLLDVRNHTSNDLEFLKMVIPNYSGEKYYKTESVVSDENLV
                     TASGIAPLEFTFQVLKLLDVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(142443..143405)
                     /locus_tag="PC545_000111"
     CDS             complement(142443..143405)
                     /locus_tag="PC545_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2454909.1"
                     /translation="MAKIDNMMSILWMLNSDKKITAKQIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDTGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQNQEDILTNHLRALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEVRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQNIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIEPLSLRKKIIEDLLELIDYYQI"
     gene            complement(144167..>147539)
                     /gene="cbpA"
                     /locus_tag="PC545_000112"
     CDS             complement(144167..>147539)
                     /gene="cbpA"
                     /locus_tag="PC545_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding adhesin CbpA"
                     /protein_id="HDF2454910.1"
                     /translation="EDSNAGNAEGDANAGNAEEDANAGNAEEDSNAGNAEEDMNALVV
                     EEATAEDPTSVGDFKISGGEINVDFKFASGVLTILSGKQMTIQNNNPNTPTTHRIVIP
                     KDVKAELIFDGVNIKTASNSPFTLTPDKDGGAYAHVILQDGSENKLESGDVYYPGMRA
                     GKGTTLTIDDSVVNRDIEGEFIVPEGGKIPRDVTLENGDKLKKGDRLTLLDSNNRGSL
                     KVVGNYYSAGIGGGNTESGGNIIINGGAITAIGQESSRNTGYGAGIGGGGNGSGGDIT
                     INGGTITAKGAYHGAGIGGGYYTTTKNALSPSVVDPSTGSGYSGNITINGGLSYSYGG
                     AHGSAFGDGCVNPGTNRGYRILVTGGTILPYMTGVGDWIFDLSATTGDVIVTGGSLKA
                     TTFKSLGGAVAYGDLEKKTKVFMNKISLKYLGQDKVSTTLVDDFKMLIDGKIYPYGAP
                     SYTDDQGVLYLWFSDQFKGSEVSVNLVVIDKITGGNIKPDDFYVKDIGSGSQFLKQYE
                     EFTIDDTPPYLIIKRYDGLPFEEDVEKLFLDWILKDGGIPIITPKGEKLTDKKFMTIS
                     SQRLNEETMEPDTNLGSDDLTKQVDAGKYQLSITSTQFSNVYPFNEAYWGHRAYYKAE
                     IIPADTETSLVVEESKAKTDVFKPTDTFTLNVTISPDKKEGPDCASPRGYVQFYINGK
                     EYKDPVELKTHNKGSESHNYSTASIEWRPLDSNHHIFTTEQTITVKYIGDDINYIEGS
                     EDEKKLKLDMTNVDVDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWK
                     PSTEGGNGDGIWKPDKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTGSGG
                     YDTGNPNLNKPDPDNGGNSKPDPDNGGNSKPDSGNGGNSKPDSGNGGNSKPDSGNGGN
                     SKPDSGNGGNSKPDPDNGGNSKPDPDNGGNGKPDPDNGGNSKPDPDNGGNSKPDPDNG
                     GNSKPDIDTDGDGKPNINIDIDGDGKPDINIDTDGDGKPDINIDIDGDGKPDINIDID
                     GDGKPDINIDIDGDGKPDINIDTDGDGKPDINIDTDGNGKPDINIDTDESLDSNVGQN
                     VQTGDQSNIMLDLALMFISLFFLIKNLTNKYLRRK"
BASE COUNT        48111 a        26150 c        15672 g        57606 t
ORIGIN      
        1 atgatttttt atcttaattt tctaaatata agtgaatgct atcattttaa gtatcttata
       61 agcaatgtaa ctatatatct ctaataacta cttttttaac atgcttaaac tatttataag
      121 ctattatatt tatgtattaa cctaataact ttttcactaa aaaataacaa tctattcaaa
      181 gataaactat agtacttctt ctactttgtt tcaataaaat actaaataaa tttcgtacat
      241 tcacaatttt tcaggctaca ctataaatat acaatattta tcttagttat aagtttaaga
      301 atatttttag tcttattttg tcgaatatct ttttaataaa acttcataat tctaagcata
      361 tttttacact gtgttttcat gtacaaaaat aatgcttaca aataaacaac ataaataaaa
      421 cttaaataag atggattcta ttcaacatag tatccatctt attcattcaa aacctaaaag
      481 gattttttat aatattatat attttaaaat ctaagtaact cattatagta tcaatttcac
      541 ttaaagctta caatacactt attaatcttg ttttatatta aaaacatcta caataacaat
      601 ttaaatcttt aagtgatatc catctcacac ctaaaggagt aggctctccg cactattctg
      661 taattcttca atacaatcat tattattttt tatataattt aattatatat tttattttac
      721 caaaaccagc atacataaat aatacttaaa atagattaaa taaaggatac taaaacactt
      781 tattgaggta tatgacttga aatatatgaa ctaacttcat taggagaaat atctaaaata
      841 gttgcaaatt cttcatttaa taacctttca gcttctttca ttatattatc atctgcttga
      901 tgtgtttttt taccaataga ttttacatac tctctgttat tatctatact tcttactaat
      961 tttattaact cttcacattt accacctctg agtatttttt taaattgctc acttctcaat
     1021 ttttcattat ctatccatga tgtatccata ttaggaatat catttattaa tgaaagcaca
     1081 ttaattttag aaagtaactt tctcattggt actttttcat tatctacagg aatttttatt
     1141 actgtattat tagaataaat aggactcaat acataatact ctttttttat tccatttgta
     1201 aatcgttcat ttgttatatc catgactttg cagactcctg tcataccata cattatataa
     1261 tcatcaatct taaacaaaaa catcaccatc cttaataaaa aaaatagttt tgacaactaa
     1321 atccaccttg ccaaaactat ctgctatata ttatatttta acacatttta agacttaaat
     1381 gtaagtaaaa cttatattat taaaattaaa aaatacatta accattgatt aatgtatttt
     1441 ttagattttt ataacaagca tatcaaaata aattaataaa gcttatctcc tgtgaatacc
     1501 tataaaattg gtgcttggct attcattgag tacccatcat ttcttacatc atttatttct
     1561 aatatttcat catttgtttt aaccttagta agttttttaa ctgctccata aggttctttt
     1621 aatgaaattt tatctcctct tccagaaata acccataaaa ttttataccc tctaggtatt
     1681 acttgtagtt tattttcacc tttaccatca gtaaagtaga ctaacaaatt aatcttttta
     1741 ttattcgcat actcaaagac tggagaaaat ctagtgcctc cacctatcct aattctatct
     1801 tttatatctt tttcagttct cactttatat acacgtctaa tttcattatc acattctata
     1861 attgtaattt catgattata gtttttcact atactaagaa cttctttaat agcttgttta
     1921 aactcttcat cactaatact tccacttaca tcaagtgcaa ctgctatttc tgacttatga
     1981 cttctaagtt ctcccctcaa atctaaacga tttggttgtc ttctatttct tcttgttata
     2041 gtcttctttt tattattttc aactgttccc attattctat taagatataa attccatggc
     2101 aattcaccct tactattttt aagtgatgat atcatacttt ctaaataagc aggaacttta
     2161 cctttttgag aactgttaat aaatttttca gtaaattctc taagggtatt ttcatctatc
     2221 tcattggatt catcccatat atcatgagtt ctttcggggc tataatcaat ttctatatct
     2281 ccatatttat cactatcatc ttcctcacca tcttcatcca cttccatcaa atctaattca
     2341 gtctgaatct tttctacata atattcaaaa ggttcatatg gttcaagttt taaggaatac
     2401 tttaaattta cccactctaa ggtagttgca tatggtggta aattgtttag aaatttattt
     2461 actactatat ccattgccat attaattgct aatgtactat actttccttt taactccttt
     2521 gctctcatca aatgcataga taatatatga tgaatttcat gtttaattgt agtctccatt
     2581 tgctttatgt caagcgttaa aaagattatt ggattaaaat atataacata tttagctccc
     2641 ttaaaattca cacctgtagg actacttata tcaaatctta tttctcttga catctgaaac
     2701 aaaaaatatc cataaaaatt atcttcttct tccataagac ttaaattcac tttatctaca
     2761 agactaaaaa actcatactt aaaatcttgt ggtatatcta tatcaaattt ctcattagca
     2821 cgatttgcct ttagcataga atgagtattt ataatttcac ttgctttatt ataaagctct
     2881 tttgcttgtt tatcaaaata attttccata cattatcccc ttattaaact ataagattca
     2941 aaatatgatt ttacaaactc ttcattttct atagcttttt tatatacttc aatataactg
     3001 tttctaatat ccttcataat acctatcatt aaatctacag gatacatttt taaaaattca
     3061 ataaatctat taatatagtg gtttgattca tatttatcat tcttaatatt taattctaag
     3121 tcttttagaa tgttcatagc tgatagataa agtcttgtat gactctcatt ttttactctt
     3181 tctacaatag actcatcaat agattctcct aaaaaaacat cttcatagga tattaatggt
     3241 tcagagtttg attcaataaa acttataaat tcttctgcaa taactttacc tacattaccc
     3301 tttacaacat ttaaaaatac tgctctagga atagaattat ttttttcttt ataaacttta
     3361 taaattttag atactctctc ataacttcta ggagtggctc ttacatcatc ttcatttatt
     3421 ttatgcagat attctggaaa agtggaaata aactcgataa cttttctttc aataccctca
     3481 tctattgccc attttatcca ttgtgtatgg tcactctcca tatttaacca aacaaaccta
     3541 ttttcttggg ctgcatccat atctaccact tgataatcaa aatctgaacc atatttactt
     3601 gatggattca ttgctgctaa aatctttaca tcatcatgca gtttatatcc attaatttct
     3661 ctatttaaaa ttaaattcat aagttcttgc tgtactgtat gctcacaacg atttatctca
     3721 tctataaata aaagaactgt cttactttta gatatctctt catcaatttc tcttaatttg
     3781 ttatgaacag catacactgt tgttttcttt tcagtcttat atccattaga attaactcct
     3841 acataagatt ctatagttgg aagaccacct atttcaccct ctttaagtaa gtttccatca
     3901 ataacaatta aactccaatc attttctttg gcaagcttat ttgctaaagc tgtttttcct
     3961 attccacttt cacctactat taaaggtact tctccagtgg ataatattaa gtcaacgctt
     4021 tttaatgtgt ctataaaatt catttttctc tctcctacat aatctttaat ttctcatcta
     4081 cagctatttt ttttgctgtc ttacttccat atttataaat aaacataatt ttatctattg
     4141 gtatactttc gctatttcta tctataacac tacaattcaa aaaatctcta atatactttt
     4201 cctcaacatc ttctttggat aatacttctt ttaatacact ctctaattca tctttagata
     4261 cttttttcag aatgtactta atcactaaaa tattcacttt taaaaattcc aacattttat
     4321 ttttatctaa atcagttata aagctaatag ctgcttcatt attttttata gctataagtt
     4381 ctgcattgta actaggtgaa tctatatatc tcaaggcatc ataacttatt ctcactgcct
     4441 cctcttgtac actttcataa ggttgtttta taaactttat cgaatcataa ttttttctta
     4501 ctgctaatag ttgtaattct tcacttggat ttttagcata tttaattgcc catcctgctt
     4561 gatttatagc catttcaata actttatcag ttgggttttt gatataatcc agtatactcc
     4621 atccctcatt tactgctttt atcatcatat cttctgttgg attatctata aatttaattg
     4681 ctctaatatt attatctatt gctagttctt gcatctctct ggttggatta tttatatatt
     4741 ctagtacaag cccattttct ttaatagcta acaatttcat ttcatcagtt ggattatcta
     4801 ttgtagctat aatattggga ttatttttta tcatttcaat aactctcaat ttttccataa
     4861 tgtgcctttc cttctataat tatttttata tctatattaa ttataataat ctattcctat
     4921 ttatataatt gtttcttatt ataattaata tactttttaa tcaaatttaa acagaataat
     4981 tataccaata tataaaaaaa tttctaaaaa acaataaaat taagttataa acaccaatat
     5041 atttctatta tttcaataag tatacaatta gattagaatc taacgctagt aataaaaaat
     5101 tactttccca aaatagatat aagcttcatt ttatcgttca aataatgaaa atatatttga
     5161 tttcatgcaa aaagagtgcc taatgaacta aaaagttcat tttgagacac tcattttttt
     5221 atataaattt atatagtttt taatcgttcg cattcaacca ctcagttaag acttttatat
     5281 atttctcatt ttcttcaacg aatggcatat gtcttgaata ctcaaataat tcccacttac
     5341 tattaggtat tctatcatac atagtctttg caatatatgg tgaacataaa tctattgctc
     5401 cactagttac tagacaaggc tcttttattt catgtaatct atctgtaaat tcatatccag
     5461 aaagagttcc tgtaggagaa aattcatttt gtccccatcc tacaatatat gcttcagaac
     5521 ctgatttttt aggtcttctc aggcactctg gagaatcttc tgtcacttca cctgcacaat
     5581 acatttccat aaatctttct aatgcatcat tatattcttt gctagagtaa tctccagtat
     5641 ttactgcatc aagtaatgct ttttggtcaa cctcactcat atatgatatt cttctttttt
     5701 gttctttctc ccaaagtttt gcagaagaaa gagtagatga aaggatgtaa gacttaatac
     5761 cttttggttt atattcaata gcataccaaa tagcctgcat acctccccaa gattgaccta
     5821 acagatgaat ttcatcaagt cctagatgtt ttcttagttc aataagctcc tctatccaag
     5881 tatctgcatt aaatagctca ggatgccctt ctacaaatga atttccacaa ccaatttggt
     5941 catacattat tacttgtcta ccactttcag ctatcttatc caatacctca aaatagttgt
     6001 gtgtagaacc tggacctcca tgaagtaata ctaatggctt cttcccttcc gtattttctc
     6061 ctactatacg gtaatacgtt ttaaaacctt taaaaggcat atatccttca gtaattttca
     6121 tgtctatctc ctccaatttg taattataaa caataatcaa gttcattaac aatactattt
     6181 aaaacctctt atacatattt gattatgtat tttaatcttt atacctactt ttataaatag
     6241 tataacataa aagttaaaaa tctctatact aaaccaatta taaaattata ttaactatat
     6301 aattttattt tttaataata gtatatacat atttgattat tttcatttgc tgcttaaaat
     6361 caattttaag ttttcttcct ataataaact tcctctttgt ggtctcatgt tagtctctaa
     6421 atttaaaatt ttacaaaaaa ttttcataaa ataaaattat attgattgta tctaaacaaa
     6481 tagggcaata aaataattaa gtatagttac ttacctttca agcaatatta gttttatgtt
     6541 agaattttat tatattgatt tacaggaggt ttgcatttga ataatattaa ttcacttaaa
     6601 gtaataaaat tatctcttgg atgttctctt gctatattta ttgcttggct attaaaatta
     6661 gaatattcaa tggttgctgg agttatagta ctacttacag taaaggacac taaaagagaa
     6721 actcttaaag gctccattgg aaagatttat ggttttcttt tatgtactat tttttcatat
     6781 ttatgtttta atattttagg atataatttg acttcatttt caatatttat atttataatt
     6841 ataccacttt gttttttgtt aaatattcaa gatgttattg ctatgtgtgt agtcatagca
     6901 tcccattact accttcaagg agaaacttct atcaaatgga ttttaaatga agctggaata
     6961 tttgcagtag gaactggaat aggtgtctta ataaatatgt atattccaac aaatatacat
     7021 aaaatccatg aagttcaaaa aaagttacag gaagaagtga gtattgtact tatagacatt
     7081 gcagatatta ttgtaaatcc taaaaaagaa aatggatata gtagagattt atatacatta
     7141 aattcactta ttgacagcag tatttctgaa acttacgata atattaataa tactttatta
     7201 agtgatacta gattcttctt agagcatatg gatattataa aaagtcaacg ggatatctta
     7261 gaaaatttgt atagttatgt atctcaacta aattctacac caccacaagc tcatattcta
     7321 tctgctttta tacataagat aggttatact gaatttgaag ctgaaacagg caatcttctt
     7381 cttgaagaat taaagagact aatgcttagt atgaaaaacc agcctctacc tgttgacaga
     7441 acagagtttg aaaatagagc tattttattc ctatgcttga ctgagttaaa acagttttta
     7501 gttaatcgta aacatgctca gatgcttaga gataataatt tttataaata aaaaggggag
     7561 aaatctagca gttgacctga ctacaaaata tcagatataa tattaaatgc gcccctagta
     7621 agagggcgca atgtttataa cttattctac tgtcatgatt aaatcattat tcatatcgaa
     7681 catacattat ttgaccaagt ccaatattag ctttttcatc agtgatttct attttaatag
     7741 tcacatcatt ttttttacag tacttcgcta attctgcacc agaacgattt ttaccactaa
     7801 aatctaaaga aaatggctta tcatatttaa aaggatattc aacaggttta ccctctggtg
     7861 ctaatgcaaa catcccatat ggtgtgttca taaatcctat tacagcaatc gtaagatttg
     7921 atgggtctaa atttgattca ctacctgtaa atgtaattgt atttactgat gaattaacta
     7981 cactacatct aacaccttca tacattacat gtttttcatt tatatcttct cctttttttc
     8041 caaaatttcc aacagccttt gccccttctt gatgtggtaa catagtgtgc ttcccaggtg
     8101 gtgctggttt ctgtgtctgt tctggtttta ccgtctgtac tattgcacca acttttggat
     8161 atgctgtctt atcaaatggt aatgggtggc taagactctt gtatatgata tcctttcctg
     8221 ctgatttatt agcatcaata tctacatgat acaaacgccc atcaccaaaa cggataagat
     8281 tccactggtg gtcatttgat gtgctgtgta cataataaga ttcaattccc atagcatcac
     8341 acaaagcttt catgctccta gccattcctg agcatgcacc ttttttagta accaatgtat
     8401 aggcagtatt tgctccctct actgcattcc agtcaacata tgttgtgtta tttttaatgt
     8461 gattgtaggc aactaaaatc ttagcatctg tggtcatatt ctttgtgatt ttcttgttca
     8521 taaaatctgc tgttgcttgt gctactgagt ttagttgggc ttgtgacagc tttggaccat
     8581 atacgctatt ggttccagtg acatagcttt ttggtgcttt tgctgttacc tctttggctg
     8641 tcataccata aactgtcata ctcatacctg agattaaaac tattgttgta atcaataact
     8701 tggtagctaa cttcctaatt ttcttcatac ttaatattct cctttataaa acattgttac
     8761 ttcctattct ctaccattaa taaaattaat ttaggattta aaaaggtgga ttgtcaacat
     8821 gtatatttct ccattcatac attcgtgaaa tatttaaatt tcatggaaaa tctaatagat
     8881 attttctaga taatttgtat acaatacaac tcgcaaatca gggtgtttgc tgttagtttg
     8941 agaaaatgat gatgattaat acattcggaa tctttgacct ataataaata ccattgtctt
     9001 caatcattaa taatggtaag agtaaagaga attatcaata atacagttaa aatcaacttt
     9061 gtatacattt catacattct ctcccaacaa cattatactt cttataattt gtatattcaa
     9121 tacgttttct gggaaaatat aggatatatc tataaaaata taaaaaagga gttgaactta
     9181 aaatttgatt aaaaaaaatc aattttaaga caactcattt gtattataaa aatgctatat
     9241 attattatta agtaagtcaa tattttagat atttatctat ttcttattga cttacgctta
     9301 tagcctaact tatttgttta gttgatatat aacctagttt taagcattat taatatttaa
     9361 ataatcaagt atcctttttg tttcttgtag tttctgttct gcctcattta agttttcttg
     9421 aattatagaa atatatatta tatctatcaa cgtaagcatt gctattcttg aagaaatagc
     9481 tccttctcta agtctttttt ctatatttgg tactactagc tttaaatcac taatcttact
     9541 aagatgattg tcactatatt tagttataca aatgacctta catcctttta attttgcatt
     9601 ttctatagac tttattactt cttttgattc tccagagtaa gatattgcta tagctatatc
     9661 ttctttatct acatttgaag ataccataag ttgtgtatga ctatctaaag aggtaaatgc
     9721 ctgtttattt atccttaaaa gtttcatctg taaatctagt gcaactaaag ccgaaccacc
     9781 tactccaaat atatatacat ttttagcctt tattatctgt tcaactgctt cttttattat
     9841 atctttttca ttcaacaacc ttgtttggtt gatagtctct atgtttttgt ttgtaatttt
     9901 gtttataaca ctatctatat tacaatcata gttttcatct acatatgtgt attcattttc
     9961 tttgcccatc aaatctttag ctatctcaat ttttaaatcc ccaaaacccg aatatcccat
    10021 ttttctagaa aacctaacta tacttgctgt acttgttttt gagtttgacg ccaattctga
    10081 tacagaaagc ttacttatag cctctgtatt attaagtatg taatcagcta tttttttatc
    10141 tacttccgtt aaatcttcat atatctgttt taattttaaa atgcaactca tactctacca
    10201 cctctaccta tttactataa tatttcttct tagcaagtaa acatgctcct aaatctggag
    10261 tcaacactgg tctcactata ccaaatctcg ttttattcaa atatttttta agtggctcta
    10321 atattaaatc tcctgactta aacactccac ctgaataaga tacctttatc tttgtacctt
    10381 gttcaaaatg atgatctaat cctattataa gcctacttaa ttcataagct gcatcatcaa
    10441 atattttact agcagtatta tcaccttcaa taactgcttt taaacatatc gtagcaaact
    10501 ttgctatttc tattctatct ccttgtattt catcatttac atattttatt atgtcataat
    10561 cattctctat attaagatgt ctttttatta aatcatataa ttttgttttt gaacaccttc
    10621 catcagactg cattgtgaac atttttaagg tagccattcc tatataatac gctgaacctt
    10681 catctcctaa tacatagccc catcctccac atctgtgaag attcccatct ttatctaaag
    10741 cctgtgctat tgaaccagta cctgcaatta tatttatacc tttttcccca tttaaagaac
    10801 ctgctaatgc tatttctaca tcattaccta gagtataatt tatatgacta tatgcatttt
    10861 ttgttgctac ttctaatgca tacaatactt cttttatttt tccatatcca gcaagtccaa
    10921 caaatgcata cgttatttct tctacattta tcttagcatc tttacaaact tcttccaacc
    10981 cagttattaa taatttcgtt agattatcaa atcctatttg attataatga caagttcctt
    11041 tggttatggt tcctactata tttaattctt catctactaa tgtaaatgtt gttttcgttc
    11101 ctccaccatc tacacctaaa aaatacatac tatcctccta caatatcttt aataattctt
    11161 tcaatactta atatctaaaa ataaccttcc aatgctgttt ttttaatatt gaaaggttat
    11221 cagtactatt atttatttta caatttttct attgatttta aagcttcact tatataacct
    11281 tcttgtctat ctaggatact ctttgcacta tccttatcta aaccactttt tatcataaat
    11341 atagccaatt taacgctatt atctgcttct tctaagaatt tttctgcaac gtcatatttt
    11401 acatttgtag ctatcattat tattcttttt gctctttcaa ccaacttttc atttgatgtc
    11461 ttaacatcta ccatcaagtt tgagtatact tttcccatct taaccataga agctgttgtt
    11521 aacatattac ataccatttt ttgagctgta ccacttttca ttctagttga gcccattatt
    11581 acttctgccc ctactataat gtttattggt aaatccgctt ttttactcat ttcactattc
    11641 ggattcatag ttattgatag tactgcacac cccaattcct tagcatactc cattgcacct
    11701 ataacataag gagtccttcc tgatgcagct ataccacata taacatcctt agatgtaact
    11761 cctctatcta ttatatcttg ctttccaagt tctttatcat cttcagcacc ttctattgct
    11821 ttaaatattg cttgatttcc accagctatt atagcttgaa caagctcaaa atccactccg
    11881 taagttggag gacattctgt ggcatctagt attcctatcc tcccactggt acctgctcct
    11941 atatagaata atctacctcc ctgcttcatt ctatctacta ttatattaac agcttttgct
    12001 atactctcct tttgttcttc aacagcatag gctacagttt tatcttcgtt atttattaat
    12061 tctattatgc ctaatgtatc ttctttatct atttgcaatg tattcttatt tctaccttct
    12121 gtaactaaat tttctaaagt ttttattaac atatcatttc ctcattttct taatattaaa
    12181 tttttaaaca cattttaatt ccaactttca taattatatt tttataataa attaatattt
    12241 tcataataaa ttattttgtt gagggtttaa aattaaactt ttgccatggt ttaatataat
    12301 ctaatataaa taattcttct tttcttatat atcctactac attcgattta ccactgtttt
    12361 tcatatcagt caaggctatt tgtaactccc ctgcataatg accatattca ctactttcaa
    12421 ttactatatc tcctcttttt attattttag gagtattaaa tagttcaaat ttgtatcctt
    12481 tatatttaac acgagattga gtagacctta tcagactatc agatatgtcc cctctattaa
    12541 aatgtatttc ttcaaagagt atcttcttct caacttctcg tatattttcc tctattgcta
    12601 catcaaatgt aaccatatct tttctcatag aacctaatac ctttagctcc tcatatgttg
    12661 gataacaatt tgatattatt atatcattta tattattcat agctattata tgctttactt
    12721 gaatatctat aggtagattt ctgtgcatct caattgttgg aagcccatca gttacaggcc
    12781 atggtccaaa tgtagtttca ttttgacttg ttacaaaaac tgctgttctt aatccatact
    12841 tattaaatct ttctgtacat tttataaagt gttttaaatt taatccacta tatctatgtg
    12901 gataaaagtt atgacatcct atcaaatttt ctttatttgg ttggtagtcc attattgtat
    12961 ctatataatt agtattatta ctcatattga tttctatttt aagattttga ggattaaaag
    13021 tcattaatgc ctcttcagaa cctgtaaatc ccatatcaag ccttattccg tctgccttcg
    13081 tctttttaaa aaaagtcaaa tctttgtagc ttatttctaa ttcaccaaat actctgggat
    13141 ttacatcaag tattacctca aaacctttaa acttagcgta ttcatttata cgtttaaatt
    13201 ttttaattat attttcttta gtatctttaa ctgataataa acatgaaaat atacgtgtaa
    13261 atccattttc agcagcttta tctatataat catatatttc cttagatgtt gttttttctg
    13321 gataaattga tattcctaat cttcccatct gtgtcatctc cttatatcct atatgtgttt
    13381 aaatttattt cgaattacac gaaacttttt taaaaatttg gtgaaaaaaa gtttcatata
    13441 tatttataaa aaaagaaaag tttacctttt ctttttttat aaacaatcaa caaaatatct
    13501 tcgttactta tttcataact gttatacttt gaaatttata ctaatctaat atagttttta
    13561 atttccaata tagtttctaa ttaataagct tcttgctctt caactttttc ttcatgaagc
    13621 aatctcttat cgtacatctt gacaaatgga tagtaaatta ttattgatat agctatacat
    13681 aataagttaa gaactgaagc tctccaatct ccaccagtgg ctaaatatgc ccctactggt
    13741 cctggtaatg tccaaggagc agtaactact actctggata ctaaattagc actcatagca
    13801 aaccatgaaa ctgttgccat tactattggt gctacaacaa atggtattat taatattggg
    13861 tttaatacta ttggtgctcc aaatattatt ggttcattta tattgaatat tgctggtatt
    13921 acagttgctt ttcctaaaga ctttccataa gctgattttg ctctaaatgc aagaagtaaa
    13981 gctaagccta ttgtagctcc agaacctcca atccatataa accattgaaa aaagggttca
    14041 gcagcaatac ctggaactgc ttgacccgca gcatatgaag ctgtattagc atctaataat
    14101 tgtatccata aaggtcttgc caatgaacct attattgata caccatgtat tccaaatgac
    14161 caaaaaaacg ttattaaaaa tattagtata attactgatg gtaatgtgtc agtggctgaa
    14221 actaatggag ttactaattt agctatgaag ccatgccaat caaaccctag gaaataagta
    14281 acaatagcca ttaaaattat tattaatgcg gctggtgtca gagcttcaaa agaccttgtg
    14341 actgatgctg gaactgcttc tggcatggtt atcttgaatt tagatttgtc agtaaatcta
    14401 taaatctcaa cacctattat agatgttaca atagcaacaa acattgaacc tccaccaaga
    14461 ttagccattg gcatttgcca tccttgagga attgaattat accaagctaa aagttctgga
    14521 ctcttttcaa ctatctttaa taactcaggg gtaagaccct ctattgcagt aggaactatt
    14581 gtcaaaagga atgtagctgt agctaataaa cctcctgtca atccatctaa cttatatgac
    14641 tttgccaaac tataacctat gccaaatgta gaatatactg atataataaa aaatgtcatt
    14701 ctatatggta gtaatattgt aactgcattt tcctttagat actgagttac cccccatgaa
    14761 tctggtaatg gaggaaatgc taatatcaag aaaaatgacc caactattat aagtggtaat
    14821 gaagctacga ttccatctct tacagctctt aagtgacgtt gttctgccaa tctggccata
    14881 gaagttgata atttagtgtc taaaaattct gtaaatttat ctagcatctt ttctctcctt
    14941 aattaatatt tatttttatt atcattaaaa tttttataaa atcaactttc ttctaatcta
    15001 acaacactgc aactactatt tactcaacag ttcttgaact tgttttaata actttggtcc
    15061 tcctaatgga ctataagctt gtgcttgtat taatgcacat ggtacactaa cctcatcagc
    15121 aaaagctttt aaatagtcaa atctatgtct gacttgtgga gctaccatag caatatccca
    15181 tccattttta acttcattct caaactcttg agtaccaaca gccaaaacat ctatatctat
    15241 tccatttttt gctccttctt tttgtaatgc attaactgct atagcacttg acatccctga
    15301 tgaacaaaca aataatactc tcatattgat tccccctgtt tttttatttc ttttcaatca
    15361 ttatagcatg tatttttgat tacaggaata cgtttgtata tattttttca tattttacat
    15421 tttaaattgt aattttattt catgcattta tatcttgctt taattgcttt atttatctaa
    15481 tcattctttt gtctaaaatt acacactact taaaatactc atatcttttc ttaaaaaaga
    15541 aaaaagacta tcttattatt aacataaaat agtctcaata cttttttaaa tatgtattcc
    15601 tttatatatc cagaagtaac ctattattgc aaatgataaa gcaaataaaa tatacagtct
    15661 aaaaaatccc aaagaaaaac ctctcttagt ttcaacaaag tatcttgtat atgcaactgc
    15721 aaaagatgta attaatgaat ttcctataac aactccaaat ctattatcta ttccaaatat
    15781 agataataac aaaggaaaaa ctacagtact tacaagagat gaaactatta gtattaatcc
    15841 acttttaaaa cttatagttg gaatattata atctgttctt tgatttttct ccatttgttt
    15901 cttagtattt ttattacttt ttgtactttt tgcattttta taattattat ttttctttct
    15961 tttctttttt ttattattag acatgtcatt tacctccaaa aggtttaggt gttatattcc
    16021 ctaaaataac tttattttta gcccctgttg cagatattac attagaaaaa gaattattta
    16081 atgtttcatt gcccaagatt acaaatgcca aagcctcctt tgcatcagaa gaatacccat
    16141 attcatcttg agttaatact tcaacctcta ataataactc ttttatatat cctattaaag
    16201 ttagattatg agctcctcca cctccaatta ataaagtatc tacattaacc tttggaagta
    16261 tgaagttttt ataattataa tatatagctt ttgccgtaaa catagttact gttgctatca
    16321 aatcatattt atctacatgt ttatatttgt taagcatgtt atccaaataa acttgtccaa
    16381 atacctctct tccagtgctt ttaggtggcg cttgactaag atacttatga ctcatcaaat
    16441 cttttaacat ttcttcatta attttacctt ttgatgcaaa gtatccattt ttatcatact
    16501 ttctattaaa taatctttga catactccat ctattatcat atttcctggt cctgtatcaa
    16561 atgcaaatac atcatctatg ttacatgatt taggtattat agtaatattt cctattccac
    16621 caatattttg aagtgctaaa tttttattgt tatcactata taataaaaat tcactatatg
    16681 gtactaatgg agcaccttct ccacctgctg ctatatccat tactctaaaa ttagatatta
    16741 cctttgtatt tgtttcatat gctataactg ctggttcacc tatttgaagc gttgaagata
    16801 caaaattatt ataacttctt ggtatatgaa agattgtttg accatgagat gcgataaaat
    16861 ccaaatttgc tatatgaaag tttgctttat gacacactga ttttacagct ttactaaata
    16921 aatatccaag cttaaaattt aagctacaga ttaaatcaac actcgattcc tcttctatac
    16981 agcactttct tatttcgtct tttatatctt taggaatttc tagagttata aattctattt
    17041 gcttaacttt agtatttcta ccattcccac taatttcagc tagtactgca tcaactccat
    17101 ctaaggaagt tcctgacatt aaacctacac tatacatagt tatcatcctt ttaagtttat
    17161 agttattcct ccccaaatca aggtattaag tttaataatt ttagaattaa tcttatttca
    17221 tctttaatta cgataattta ctcttacgaa attaatcgag ttaatcttat acacgttttt
    17281 taccttagct aaatcttaca ttaatttatt ttcagtgtca atgtaatacc atgtaatttt
    17341 ttttcacaaa caaaacatat tctctctcca aaaaccatat taaaatagtt actacaaata
    17401 aaaaacatac caggtaccta tctgatatgt ttttcatata taataattat tattttataa
    17461 tatagatgga gcatcattta aggcactaat taaatcttca ctattttcaa gaactaaatt
    17521 tattcttatt gtatatgtcg atttgtatat gatgatagat tttatagtct aataacctaa
    17581 ctatctatgc ttaaataatc cgatatatca taaaagtaga tattaaaaat tgtatatgaa
    17641 ccaattttcg aacatacctc aatctcatgc cctaaactat ctatcatttt ctttacaaaa
    17701 tacatcccca ttccagtaga cttatacaat tgatttcttc catttttccc cgtaaatccc
    17761 ttgtcaaaaa ccctagatat atcttcctca tttataccta taccaaaatc ttttatagat
    17821 aataccactc cattttctaa cttttttgaa ttaaattcaa tcttaccaac ttcctttgaa
    17881 tactttatag cattattaat aatttggtct aaaacatatg acatgcattt agaatctgtg
    17941 taaacatcat tttctaattc attcaaacta acttctacat tattttttat aagcaaaaat
    18001 gaattatttt ttatagacat ttttaccaat ttttctaaat taaatttatt tataaataca
    18061 tcttcaaaca tagtgttgct tctactacta tacaaaatag agtttactaa aaaatttatc
    18121 tttgcaacct gattcttaat atccaaacta tcttctatgc tacttaacct atctgtaatt
    18181 atacttaaag aagatattgg aatttttatt tcatgaaccc atttagctat gtactcatcc
    18241 aattctttta atgattgttt ataagtctct atgtcactat tattttttat ttttaagttt
    18301 tctataatat cttttattag attttcttca aaagattgtc catcaactaa ataactatct
    18361 atttcaccac tattttctat acaattatat aaatttgtgt aactatttct aaaatttata
    18421 taatctataa caaaaaatac cattattata aacagcacta aaaaatctaa atatattaac
    18481 tctgaatatt tatttttaaa aatatctata gcaaataagt agatatttat tatactaact
    18541 acaactaaag gtagagcaat atacttagac ctacctttca aatattttac tagcattgta
    18601 ttatatatcc tatccccttc ttagttttta taaaatcatc aaaacccagt tctttaactt
    18661 tacttctcaa tctattcata tttacagtaa gtgcattgtc tgtcacaaac tcgtcattat
    18721 cccatagagt catcataaga ctttcccttg atacaaccct attcttattt tttaataata
    18781 atgttaaaat ttttatttcg tttttagtca actctatact tttatcctta tacttatgtt
    18841 ttataatccc tttccccaca tcaaacatca aatcttcaca atacaaaata ctattagaat
    18901 cactataatt atagactctt cttattatac catttatttt ggtaaccaat atttctatag
    18961 aaaatggttt ggtaatgtaa tcatctgctc cattatttat gcccattatt aaatccatat
    19021 cagaatctct tgatgataaa aatattaatg gtacatttga aatagctctt attttttcgc
    19081 accagtaaaa tccattatat aatggtaaat ttacatctaa aagtactaca tctggttttt
    19141 tatctataaa ttcatctaat actacctcaa aatcatcaat taattctact ttaaatcccc
    19201 atttttcaag tgctaacgca atctcttttg ataaatcaat atcgtcctct actaataaaa
    19261 ttctatacat ttgtatattc tccattatcc taatatttca ctatgctatg aattattaat
    19321 atcataacat attttatatg aatttattac ccaaattatt tagttaatca acaaggagac
    19381 tcttattaca agaatctcct ttctctttat taatatatca actaattttt atacactaca
    19441 ctcttaaact tattaacaga tactgctcca tatattatga atattatcaa acatatcact
    19501 aaacttagta tatatatacc aaataaatcg gtcatcaata aatctcctgc taatctcaat
    19561 gcaaaataac tagatatagt tgcacttaac attggcaaag tataaaaaat agctacttct
    19621 ttaagtatag ctctatttat atctttttct gtagtaccta tttttaacaa aatcttatat
    19681 ttctctttat cttcatatgc atcactatat attttcatat atattatact tccagctaca
    19741 attgcaaaga ctaagaataa aaacacaagc actacatatg caaacttcat ccattgactc
    19801 tcaccttgag ctttatatcc attttgaata tacccatttt ttaaacttaa attcttttca
    19861 atattgttga acattacttg actattttct tcttgaaata cctttgcacc ataaaaatac
    19921 acttgttgac ccttatctct tagtttaaaa tatgtatcat catttaccac tataatactt
    19981 tgattaagtc ctgtccctag tactggaact tttacttctc cccttgaaat attatataac
    20041 tgaccatcaa gttttatctt ggcttctttt gtagctattg atgctatcgt ttgagggctt
    20101 tttaactcta ttacattatt tccactaact aatctttcac catactcttt taattcttta
    20161 tcattaccat tcactttaag agtattacaa aaatcactat atttcattat agtatctgtt
    20221 gtatcataag cttcttttat atctgttttc tttaaaattg ttagttctgg atttactgag
    20281 tactttatct tattagtatc ttttattata ttctctattt gttttttatt cacttcatct
    20341 ttatccattg aaactatgct aatagtgtac acatttcttt gttcttgggc attcttatgt
    20401 atatgcttca tagcaactga agcaccaaga gttgatattg ttgtagttat aattatagct
    20461 attatagcat aagttttata gtttttattt aatcggtagt ttatattgtt tattgcaatt
    20521 atattatttc cattaaataa tattttttta tttttgatta taatatcaaa tataacaggc
    20581 ataactgatt tgaataatcc ataagtacct actattatta tacctataac aggtagtaca
    20641 ctagttatat ctccagttct tatattggta gaaagatagt agccatatcc tattactata
    20701 attgatacta tagctaatat atatatacct attcctacct ttggtatttt ttcttgtttt
    20761 ttagatatat tgattagatt tattacacta cttctacata ttgttataaa accttttaca
    20821 gccattatta taaatataga aataaagatt aaagtagcat atagtatagc ttctatacta
    20881 agtccattag atacctttat attaaatcca gataacttta ttatcactac ttgaaaaaat
    20941 ctagatgtta ttatccctat aattattcct gttatacaag aacttattcc tataagcata
    21001 ttttctacaa aatatatttt tcctatagta tataaatcta atcccataaa agacaatatt
    21061 ccaatttctt tagacctatt cttaaaaaat atatttgttg cataccatat aaagaaaaat
    21121 aaaaatactg atagtatcat tattacagct actaacactg atagtgtaaa ttttttattt
    21181 tcctcttgta acacatttat agcatcacca tatcttaata attcaaaatt agttagcaca
    21241 actactgaaa aaaccataga tataaaaaat gctagataag ttcttatgtt actcttaaaa
    21301 ttattataag caattccaag tatactcact tacatctcac ctcccataga ggctagcata
    21361 tccataattt ttccataaaa ttcttttcta ctatttcctt tgtttaattt acactttata
    21421 tttccatcat ttatcatata tacttcattt gaataacttg ctgttagagg gtcatgtgta
    21481 atcattataa tagtagcgtt attttctctg tttatttttt ctaatgattc taaaagttca
    21541 tatgctgatt tagagtctaa agcaccagtt ggctcatcag caaatatgac tgttggattt
    21601 gtaattaatg acctagctgc tgcgcctctt tgtttttgtc ctcctgataa ctgatatgga
    21661 tatttatcta aatgattttc taatccaaat tttttagcta tagaaaatac cttagcttct
    21721 atctccttac ttttcttttt tcctaatgct agaggaagtg ctatattgtc cattagtttc
    21781 atattgtcta atagattata gtcttgaaat ataaacccta ttttttctct tcttaatttt
    21841 gatagttcat tattttttac ttttgatata ttaattccat ctagtattat ttctcctgaa
    21901 gtttatttgt caagtgtaga cattaaattt agcaatgttg tctttcctgc accagatgga
    21961 cccattattg atataaaatc accttcattg actgtaagag atatattttt taatacattt
    22021 gttttaaatc cttttattcc ataatctttt gatatatttt ttatttttaa tatttctttc
    22081 ataacaatag cctccagttg ctaatttttt aatatatatt tattttaaag gttttatata
    22141 aataaatcct taagtaaata ttacatttta actgttagca ttacactttt gtaacatatg
    22201 gaagactatt ataattatag tagaaatatc ttaaaataaa agcatgattt acttctttgt
    22261 ataaacaaag aaaagaagct cctaagaagc ctctaatcta tacaattatt cttctctata
    22321 aaaaaccaaa atacacattg attgtggcaa atacagatac tataaacaag tgatattact
    22381 actatatatt aaaacaatta ttcctgaaga taattcaata agactatcaa gcaataagct
    22441 aagcatccaa aaagacttaa taccattaat gtataattat acccaatatt aatgcttaaa
    22501 aaaccagcaa cagttggaat aatcagagat gaacctccca taaatatact taaagctatt
    22561 aattggctac tacttctaag ttttttagga gaaacttcaa atattaatct ttttgaagta
    22621 attaaaatta aaggagtaga aaacacttga aaaacacaca gtataataat gatatttgaa
    22681 tcatttgtta aagcaaataa tagaaactga actgttatca taatagcact taattttaat
    22741 aagtttaaac tgctatattt ttttaataaa taagaaccag ccagatatgt tggtatctct
    22801 aacaatgact gtaaagacca ctttaaagat atctgagaat ttgtagcatt taaagctatc
    22861 attttatcta ttacagtaca gttatttgct accaccattg agtataataa gaataaaata
    22921 catactagta agatatattt tttatctttt aataattcaa taatatcttt attagttaca
    22981 tttattttct tattaatctt ttcaatatca ggtaataata gtatatttat aataagtaat
    23041 actacaatta aaccaatcgc tacagataat ccaaaatatg agaatctaag aatcaagtaa
    23101 gatgctacca tacttccaat agcccatcca atcgacccaa aagtcttaat aaatgacaaa
    23161 taattattaa tattatctcc acaacctatt atccatgtat catataaact acagcaagta
    23221 tttaataatc ctcctgataa agcaacaagt aataagtata ttataaaagt tgcagattca
    23281 gtagaaaaca taataactga tgcaattcca taaactgata aactaattaa aacaattttt
    23341 ttcattgttt gatacttatc agctaatact ccaaataata attgaaataa tatattggtt
    23401 attgcataac tcgataatat aattcctctt tcaaacgctg agtaacctaa ttttgataag
    23461 aaaggtgtga attttgttga agcaaaggca aaaataatat atgagaaaaa atagaatatg
    23521 ataaatttaa atttaatcgc tttattaatc ataatcaatc aaaattgata actttaatac
    23581 cactatttcc ttcctgattt aagtttgaaa gatgatattt taccgtatta ggattctcta
    23641 caataactaa aggaatatat aaagtatttt caagtaactt attatcaatt tctactaact
    23701 tttcccccat attaacttct tgaccttgtt taacttctaa tttaaagttt tctttttttg
    23761 ttgttgcact gtccaatcca atatgaataa gaatttgaac atcattttgc aatttaattc
    23821 ctatagcatg gttagtttga gcaataaaac ttattactcc tttagtcggt gatttaagaa
    23881 tattgtcctt tgctatcata gcaaatccat ctcccattac cttagttgaa aatactttat
    23941 caggaatttc tccaagatta attatttttc catcaatgta tgaattaatt gtaattgctt
    24001 ctttttttct tttaaagaac attatagaca ctcctctaca taattaactt tattctaatt
    24061 atcattacaa ataacgatat taagcatact gcaattgagg aaaaaagagc cttatagctc
    24121 tttccccatt gtagttcttt aatactgctt atgatttgtt taattccaac aaatttataa
    24181 atataggtaa acatgataac aatagttatt gtgtaaattt ggaatatctg ccctaataag
    24241 actaaataga aagcaatcat gttttctttt atctctttat tcttttttat attgtaaata
    24301 acatatgcac tgtatactgc actaaagtta gagattaaat ttaaacttgc attaacatca
    24361 ttgtttaaaa gttgtgaaaa tgaaatattt gtaaataatg atgtcaattc taaatacact
    24421 atatataaaa caggtaatat tagaaaacaa accataacca ctgaaagaaa tatattttca
    24481 tcttttaata ctgctgtgat attttttatt ttatttgtta tcatgttttt gttctcctta
    24541 tttaaaagtt ggccagaaat ccttattagc cacaatcatt tcatctaaga tttcttttgc
    24601 tacttttgca cttggaacag ttttactcat agtaaacgac tgccataatt tttgataact
    24661 tttttctatc catgcttcta cagctaattt ttcagaagca acttgttgtt ccattaaacc
    24721 tttttggaaa gttggaattt tacctataga cataggttca accatatctt ttccaacaag
    24781 gcaaggaatt tcaaccattg catcatctgc aaaattttca atagctccat tattaggaac
    24841 aatcagtaaa aatctttcct gagtattaaa tgctaatgca caagctaatt caacaataaa
    24901 ttgagaatgc tcacttgcat caaaccatgt atcccttgct gtgccatttt ttataattct
    24961 ttcacattct tcgaatgtat ccttttctct tctatccata atttcatttg ctcttgtata
    25021 atttggatta gcatgttcaa cagtttcatc agccattaaa taatatttta aataagtatt
    25081 aggtaatgta gttggttcta tttctaataa gtcctttgct gctctcatag tttctaacca
    25141 actagattct ttgtgttgac cttgaggtgt aaattcctca tatccttttt tagcacaata
    25201 ttcttgtaat ttaggcatta aatcatttcc ttctttgtcc ttaatacttg tccaccaccc
    25261 gaaatgattc aatccataat atctaacatc aaagtcttta cgagaattta aacctaaaac
    25321 tctagcaaat atttcctcta aacatactgg catatcacaa atattaataa ttcttgaatt
    25381 tggtcttaat acacgacatg cttctgcaac tatagctgct ggatttgaat aattaagcat
    25441 ccaagcattt ggagaatatt tttccatata atcaatcatt tcgattattg gacctattgt
    25501 tcttaatcca taggcaattc cacctgcccc acaagtttct tgaccaacaa ccccatattt
    25561 tagtggaatt ttttcatctt gttcacgtaa agcatataaa ccaacacgaa tttgtgccat
    25621 tacaaaatca atatcagtaa atgcttcttc tggatttgtt gtataactaa atttaatttc
    25681 tggatttttc tctctaacaa ttattgcaca tgcttcagca acttttcttt gtctctcatc
    25741 attattatca tataatttaa ttgattcaat aggaaattta gcaacacttt caattaacat
    25801 tgttacaatt ccagcagtat aagtactacc cccaccagca attagtatcc tctgtctttt
    25861 tctttccatc tttccttctc ctttataata aattttcaaa ttcttctctt acacttggta
    25921 ctgaaagccc aataataatc tgaatagctt ttccattatg cactaaacca tgtgcaccta
    25981 ctgccttaaa atcttcttct ttagaaataa ttgagtcatc tttcaaggtt aatctcaacc
    26041 tcgtagcaca gtttgtaaca tctacaacat tttctgctcc accaactaat tttatgtatt
    26101 cagatgcttg ttgagaggct ttactcaatt tatttccttt ttcatcttta gtttctaagt
    26161 attcattttt agtatatagt tttgtttctt cactatcttc ttctctacca ggagttttca
    26221 tatcaaactt cttaattaag aaagtaaata caaagaacca gataaatgaa aatataatcc
    26281 ctacaacaac ttgaacaaca tataccttcc agtgatacat tccaagagga agccaattta
    26341 atacaaccca gttaataatt cctccaccaa aatcaccaac aactcctaag gcaaacgaaa
    26401 tcgtttgcaa gcacgcatct aaaaatgcat gtactaggaa taaaagtggt gctacaaata
    26461 aaaatgtaaa ttctaacggc tcagtaattc cagttaatac tgcagtaaga gtagcaggaa
    26521 tcattaaacc taatactttt ttcttttttt ctggctttgc agttacatag aaagcaccaa
    26581 atacacctaa tgaaccaaac accttcgata atccggataa tgaaaatccg attggataca
    26641 tttcctttag agtcttagct gaagtttgaa aatcctgaat atgagtagcc caatatactg
    26701 atgtaccacc tggaacaaca acactatcat aacaaattgg tgcatataca aaatgatgta
    26761 atccagttgg tattaaaatc ttttgtaaga atgcataaca ccaaactcct atatttccac
    26821 tatttataaa aaacgtttgc aaatgtctaa taacattttg aatatgaggc caaataaaac
    26881 aagttaataa tgcaactggt atcataacaa agaaacaaat tgcaactaca agtgatgagc
    26941 ctctaaataa tccaataaaa tctggtagtt ctttatcata aaatctatta tgaatataaa
    27001 tgacaatacc tgatattaac aaagcaccaa ccataccagt atctaatgtt ttaataccag
    27061 caatgcttac aagtccactt ccagcactta tttcagctgc gaaatcaact ccaaagcttt
    27121 ttccccaaaa acttaacata gaacctacaa aataattaaa agtcaaatac gttgctaaag
    27181 cctccataca agctcttgct tgttgctttt ttgcaagtgc tattggtaaa gaaatagcaa
    27241 ataataatgg taattgatta aataccatcc aagctccact agatattaca ccccaaaatt
    27301 ggtaccaaat attttctggt gtagcaagtg aacccataat tgactgattt tgaaatacaa
    27361 ttgagaatcc aaccattatc ccagcaaaag caaataatag tgttggtgta aacattgccc
    27421 ctccaaaacg ctgtattttc ttcatcatat aaattactcc cctcctcata tttttagttt
    27481 atctttggcc ttagatatct attattttaa caggttaata aatatataca atctttaggg
    27541 tacatttgga gcttattcac agtcaaataa gtttttgcac atttttatgt tcaaattctc
    27601 ataaaaaaaa agaatgacta caatttgcaa tcaatccctt ctgtaatcat tctatgcctt
    27661 ttataaatac tatatttaat aaataataac tcaatcaata taaaatataa cattgtcaat
    27721 ttataactag ggtgcatttg taaaaatgag atattagtcg ctgaaatata aattgattca
    27781 tcacttaaat cagtcaatgt attattttta aactctgtaa ttgaaattat tttagttcct
    27841 attaatttta atcttctagc aatattaagt gtattctcat tctcaccact tagggatata
    27901 ataatcataa catcattttt actgacaata ttgtaaaaag aatcatcaaa tgttcttccc
    27961 tcaaaatcat atattatttc ttgacatgat aagaacattc tctttatctg ttttgcaact
    28021 acattttgaa tatcacctga accataagcg tatattgttc ttgattcata aagaagttta
    28081 cacaatgcat cataatcttt atttttttga tcttcaacat acttaattat tgaattacat
    28141 gcaatatcaa atgactgatt ttctatgaca tgaacctttt ctgattccca gcttaataat
    28201 actttaaact cactgaaacc ttttaaatct aattttttaa caaatcttgt aatagttgca
    28261 cgagacacat tacatttcat agcaagttca tttattgaca tcttactaca atgtttttta
    28321 tttgcagtta tatatttcca tattaataaa tctgtatcat tcaatttatt ataatttaaa
    28381 aaaatcattt cttctaatct cataaactat tacccccttt aattaaaaat cgtcataaat
    28441 attataccaa tattcaacat taaattacag cgtattctta tatttactat ttagcccata
    28501 aaattgacta taaaaaatac tgctataatt gatgtttaaa aaaatgtagt tttaatttta
    28561 ttcttttatc taataattaa tattttcaag ttctagtgct tttcttatac aattatcatc
    28621 tttaaaatca gcagtccttt tacaatcttt tactttaaat tctggaatag ttttaaattc
    28681 ctcatcacat attccattct tatttaaata catacaactt gccattctaa ctattagacc
    28741 actatttttt aaattaaaca atacagggtc tatacaccct ccatctccgc cagtagtttc
    28801 accaactaaa gtcgcaaatt tagtttcttt acaaaacatt gcaaatgact ctgatgatga
    28861 atatattttt ctatcaacaa gtaagtatat attaccctta aattttgaat catctgtaga
    28921 ttttatagta taggaagtat cttcaaaatc attaaaatct tttataattt ctttaggtgc
    28981 attttccttt acacttgttg gtaaattttc tacaggattt aattttatat ttcttgcatt
    29041 tgtatattgt tttactattt cattattatt tctataaatt ctataaccat ttattttgac
    29101 atcatttttt atgagtttag atacgattcc ctgccaataa ctatcacttc cacctaaatt
    29161 acctcttata tcaataatta aagctttatg tttttctaaa gtgtcaatat aatctccaat
    29221 tatttttaaa tcattattta tagagccatt ctttgatgac attgatggta aatacatata
    29281 tccaatctga cccttaataa catcctttaa aattaattct ttttttacaa aaatatcatc
    29341 tggcattttt attttaagat tcatagagtc atatctatct acaacttttt tatcattaag
    29401 aaaatcatac caatcattat tagagtatga tttcttaaaa agttcatatc ttttcttatt
    29461 atctattact tctgtgtgtt tattatttaa atcagataat atagatgaca tttcttctat
    29521 aaattcttca tcattttcag tgttttttat tcgttttgta tattcatcct tatttgctaa
    29581 ccaatcaata ttattaagcc tcttatttac acctagatat ggatatcctg tctttacaac
    29641 attatacaca taattaaagt catccacctt ttcctcttca gacaactgat tcttttgaga
    29701 ataagtagaa ttacctaata agattataaa tgctaaaata attactaaaa ataatacttt
    29761 ctttttcatg taattctcat ccactttcag attttatatt ttaatacatt gtatcattat
    29821 tttattatca cctaaacagt taaaagaaat tcttgaagat atattccact attattctct
    29881 ttaaaaattt ttatttgtat tttcaaaatt atttcatgaa agttataaaa taacaattat
    29941 attcaaatat ctattttact gctatatcct gaaggtaaat tcttatcagt gatatactaa
    30001 aaaagatatg ctaaaaaatt tatccattat aatgacttaa atttagatac tttactgatt
    30061 tttatttatt agttgagaaa gatattgata attaaaaata ttgataacta tagaaatgat
    30121 tattttttat agaaatttta aggatataaa ctaattatac ttaagtctat atccttaatt
    30181 tatcatgatt atgagaattt ttacaaatac ttatctttgt ccgttttaga ataaaaacaa
    30241 gaacatatcc tgttatttta ttcaaacatc caaatttaat tactacacaa ttacttatta
    30301 gaaattgtat ctacttcata tatatatctg ttaagtatag cttctagcat ttcttgtctt
    30361 ccagacttat tctttataac tggcatttgc agtgcataag attctaactc tttaaatcca
    30421 accttacctt ctactatatc cttacctata ccttcactaa aacttgcata tctttcttta
    30481 gtaaagtttt caaatacctc atcttctaat aatttatgag ctattaataa tccctttgca
    30541 aatgtatcca ttcctgcaat ataagctagg aataagtctt ctacctcaaa agatgctctt
    30601 cttactttag agtcaaagtt aaatcctcct ggtgctattc caccttgctt taacacttca
    30661 tacatagcaa gtgttgcatc atatatattt gttggaaatt ggtctgtatc ccaacctaat
    30721 aataggtctc cttgatttgc atctatacta cctaaaacat tgtttattct tgctatattt
    30781 aattcatgtt ggaaagtatg tcctgcaagt gttgcatggt ttgcttctat attcattttg
    30841 aagtatttat ccagattata ctttcttaaa aatcctaaaa cagtagcagt atcaaaatcg
    30901 tattgatgtt tagtaggctc ttttggctta ggctctatta agaattggcc agtgaatcct
    30961 atttccttag cataatctac agccatttgt aagaatcttg caaagttatc catttcaagt
    31021 tctgtattag tgttaagtaa agtttcataa ccttctcttc caccccagaa tacaaaattt
    31081 tctcctccta agtacttagt tatttccata gcttttttaa cctgagctgc tgcatatgca
    31141 tatacatctg cattacatgt tgtagatgca ccatgaacaa atcttggatt tccaaacata
    31201 ttggcagttc cccataataa ctttatacct gtcttttcca tattaacttt tatatactca
    31261 actatttcat ctaatatctt atttgtctct tctaaatctc ttccttctgg tgctatatct
    31321 ctgtcatgaa aacagaaata ctcaattccc atcttagaca taaattcaaa tcctgcatca
    31381 actttaatct ttgctatctc aattgggtct gttatattat tccaaggtct ctctactgtg
    31441 cctactccaa atgggtcatt accctctcca cataaagtat gccaatagct cattgcaaat
    31501 cttaagtgtt ctttcatagt cttattacct accacttgtt caggattgta gtatctaaat
    31561 gctaattcat tgtcagattt tactccttca aattttatct gtcctattcc tttaaatatt
    31621 tcactcattt catgtcctcc taatatttta cacatataat atatgtagta tgttttctta
    31681 atttaaaact attttctatc tttatttttc tttttaagct aatttaatta tttatttttg
    31741 cattaactac ttcatctaat aacttaaagt catctttcaa agatttgtat attcttttat
    31801 acaatctatg atatatattg tatgtatcta cattttcact tattggttct aatttagttg
    31861 aagtttttat tatagcatca catgcttttt gtatattttc atatattcca gctcctacta
    31921 atgcaagaat tgcaactcca agtgctggtc cttcacttga atttatagta tatacatctt
    31981 gattaaacat atctgcttgc atctgtctcc atactttact ttttccacct ccacctgagg
    32041 ctcttacttc attaacgtct atattaagat tctctattat atccatacag tctttaagac
    32101 tatatgaaac accttccatt attgaccttg caatttcttt tttagaatgg attggagata
    32161 atccaaagaa cacacctctt gcataagggt caagatgagg agttctttct cccatcatat
    32221 atggtaagta aagcaatcca ttacatcctg ttggtacttg acttacttga ttatttataa
    32281 tctcatatac atcttcacct aaagaatttg aaacttccac ttcactttga cagaaattat
    32341 ctttaaacca ttttaatgac aagccagctc cttgagtaac tcccataaca tgccatgtat
    32401 ttggtattgc atgacagaaa gtatgaactc taccttcttt atcaattttt ggctcttttg
    32461 tataagcaaa tactacacct gatgtaccta tagtagatga aactactcca tctttaacta
    32521 ttccatttcc tatagctcct gctgcttggt ctccagctcc tcctaccact attgttcctt
    32581 ctttaagccc tgtcaaactt gctacatctc tagtcacata acctgtaatc tcttgtgatt
    32641 cataaacttt acctaaaaga tttttatcta tatttaactt ttctagtact tcatcgctcc
    32701 aatctctttt tcttatatcg agcaactgca taccacttgc atctgatacc tctgtagcat
    32761 attcacctgt caatttgtat cttatataat cttttggaag aagtatttta ttagtatttt
    32821 tatatatatc tggttcattg tttctaaccc ataatatctt tgatgctgta aatcctgtaa
    32881 gtgctggatt agcagttatt tcaatcagtc tttcttcacc tactaaatca gttatctcct
    32941 cacactcttt tactgttcgt tggtcacacc atattataga gtttctcaat gttttacctt
    33001 cattgtccag cataactaga ccatgcatct gtcctgataa tccaattcct tttatacatt
    33061 cactgcttat atttgaactt tgaactactt ctcttataga ttcgactaca gctttccacc
    33121 aatcatttgc atcctgctct gcccaacctg atttctcttg tataagagga tattcatatg
    33181 tacaactttt tatagtattt cctagtttat caaataaaac tgttttagta cctgatgtac
    33241 caatatccac acctaataca tactccaact tcatccctcc ttgaatgcca cttcataact
    33301 atcgttttcc tatcctaatt tttttatatt ttaggtatat tttacttgct ttatttttgt
    33361 tagtatatta aactaactta tgaactaata ataaaatatt taattatctt tgtcaatgca
    33421 aaataacctt ttatttttgt gtaaaaagta ttacaatatt attttaagga ggaatatcaa
    33481 attggttact gataaataca ctattcgaga aatgaatgaa agattggtac ttgaacaaat
    33541 tataaaaaat ggtcctattt ctagggcaag tatagcttct actataggat tgaataaagc
    33601 aactatatcc gctattacca aaaaacttat tgatgaatct ctagttcatg aaatcggaat
    33661 tggaaatagt actcatagtg gcggaagaaa acccatatta ttagtattta ataaatgtgc
    33721 agggatttct ttaagtatgg acattggata cgactatata ttttcttctc tatcttactt
    33781 agatggaact attataaatt ctaagaaatt aacagatata caagtaagta aagacaatgt
    33841 tatacaattg atagatgaaa ttataaatag ttataacata tccaaaatag acactcctta
    33901 taaggtaatt ggtcttactt tggctataca tgggattact tgtgaaaata aagtattgtt
    33961 tactccatat tacaatctta atgagattga cttatatagc atccttagca aaaagtatga
    34021 ctttcctatt catatagaaa atgaagcaaa cttaacagct ttagctgaga atactttttc
    34081 aactgtacat aactcactac ttagtctcag tattcacagt ggatttggtt caggcattat
    34141 aataaataat aaattatata gtggcagaaa tggtatgagt ggagaaattg gtcacactat
    34201 catcatgcct aacggtaaac tatgtccttg tggaaatcgt ggctgtttag aacagtattg
    34261 ttcagaaaaa aaggtatttg aacagctttc ttctttagaa aatattccta aaatagattc
    34321 tgatatagta aagcaactat attatgaaga taaccataat gccaaaaaag ttattcatga
    34381 gttttgtagt tacttaacaa tagctataaa taatgctatt acaacttatg ctcctgaaat
    34441 aatatattta aatagccaaa taattagtga tatacctgag atattacaga ttacaaaaga
    34501 tatgttagtt agctctttta acaaaggaat aaatatagaa atttcatcat tagggtctga
    34561 ggcatctcta tatggtggct ctgctgtaaa tatcaagagc tttttgaata tacaaaattt
    34621 aactttaatt aatgaaatat aagggttatg actatataca gccataaccc tttaaattat
    34681 acattctaaa attagattta ttatatagtt tatgcaagat tagctatatt ttttaaaaat
    34741 tgtatatcaa ttaagtatta acataagtac ttcgttttaa atattaatat aatcttccta
    34801 ctcatcatca gataataaac ttgcattttg ttcaaccata gaaatattat ctttacctat
    34861 tttctctaat tttgctgcca attcctttgt atctaaaaaa gctgattcaa taaccatacc
    34921 taaatttagc cctgaaataa ttttaattcg actgtcatta ttggctacta ataaagcagc
    34981 attgcatgga gttccaccta gtaaatctgc cattataatt atttctgaac tttccttgta
    35041 cacttctaaa gcttttttta atttttctat tgttccttct attccatcat cttgtttcat
    35101 atttacagta ggataatcta tagaatcccc tattatcatt tttgcagaat ttaagacttc
    35161 ttcagccata ttgccatgac tcatcaaaat aatactattc aatttaatac acctctttaa
    35221 atctataaga tgtctacaag ataagttttc ttatcatcag gcgtcattat agttgtaatt
    35281 tcaattcctt catctaaaag tcttttaaat gcttctttct cttcttcagt aacactgata
    35341 ttaggtctta gaactgttgt atcttttcta cttgacatat ttcctacatt tatattatgt
    35401 attttaaatc ctgcctctac aaatggaata aggtccacag gagacttaac aattaataat
    35461 acattttgtc cttcatactt tcctgcagta atatttttta tagcagtctc ttctgtaatt
    35521 atagatgacg ctatacctgc aggtgtagcc attcttaaaa cttgtttttg aacagaattg
    35581 ttagctactg catcatttac taccattaat cttgtaacac ccaatttatt actccacata
    35641 gttgcaactt gaccatggat taaacggtca tctatacgaa tatttttaat acctttcatt
    35701 tccatatcta atatcatcct ttccttattt taaaatacct aatgcatata aaattattga
    35761 aacaaccatt actcctaaaa ttaagtatat aggttttata ttctttttaa gagcccaata
    35821 aacaatcaca actagaattg caggaactaa cccaggcata atttggtcta atatttcttg
    35881 acctttcatt gtaaaatcac cttgttgaaa gttcaaagca acattagctt ttacaactga
    35941 aggtattaat gcacctacaa ccattaaacc taaaatagat gtactctcag ttaaaatctt
    36001 taaacttttt tcaattgaag atattagctt agtacctgat gtataaccag cattaaatag
    36061 tggtatactt aataatttca atgctacact cactacaatc cacagtaaga ttccaaatgg
    36121 acttccatct atcgccatat tagctgctat agctccaaaa attgtccacg gaataacgac
    36181 aaataaacta tctcctaaac ctgctactgg tcccatcaag cctgttttaa ttgaagcaac
    36241 agcctcttca gtctcttcac ttgataactc ctccattgct acattaatac ccaaaatata
    36301 aggggcaacc caaggattag tgttaaaaaa ttgtaaatga tttaatacag cactttgtaa
    36361 cttttctgga ttatctttgt atactacttt taaaaaaggt agtactgcat aacagtatgc
    36421 caaggcctgc atcttctcat aatttgaaga aacagaacta gtaaaaaacc agcgccataa
    36481 gacacttttt ttcacctttt catttgattt atttaatgat accttatcac tcatcttcgt
    36541 ccatacctcc tactacagta tattgttgtt cttgattttc caaattcttt ttgtaaatta
    36601 taagtgcaat tgctccacca attaaagcta cacctaataa tggtactttc atatatactg
    36661 ctaaaacaaa accaattact aagtaactaa aatactcctt agcaggaaga tatctaagta
    36721 ataatcctat ccctactgct ggaagtagtc ctccagctac tgttaaacca cctgttaacc
    36781 attcaggtgt atatttcaaa atggtattaa ttaaagaagg tccaaatatt acagatagag
    36841 caactggaat acctgttgta gctgctgtta atagtactcc taacatttgc atataggtta
    36901 tatttttata atttcctttt ttagctccat cttctgcttt atgaactagc catataccaa
    36961 tagtatttct aagaacatct aattgtacca tcaataaagc aactggaata cctatagaga
    37021 ttcctacaga tgcttcttgc ttagtcgtaa tagttataaa tgtggctact atacttgctg
    37081 tttgataatc aggtattgat gctccaccaa agttactaat acctaatgat aatagctgta
    37141 atgtacctcc aatatataac cctgtattta tatctcctaa gataagacca gtaataaaac
    37201 ctgctgttac tggttgatat gttccaaaca ttgttgaatt tttttcatag tttataaaaa
    37261 atccatatag tacgattaat aataattgcc ataattgtgg ctccattatg atttcacctc
    37321 tcccaaaata ttttttagtt ctttaaaatt ataactggaa tcttgcttta ggaaaatagg
    37381 aatttcttcc agtgggcatt caacttcata agttttccca ccttcataac taactccact
    37441 aaatatattt tcccatgtat gtccttttgg taaatatact tttctacttc tatctttata
    37501 attcattatt ggagctacca aaatttcatc tccaagcata tatgtattat ccacctccca
    37561 agttttatca tcttcaggaa attcataaaa taaagttctc ataactggtg ttccatcttc
    37621 atgcgcttct ttcatcagtt tttttaaata agtttttaag ctctctctaa tttttatgaa
    37681 ttttgtaaga attacttcaa cttcttcacc ataagaccat atttcattag gagctccagt
    37741 taccattgag cctccacctt tatttgataa tggtttacta tgaggcaatc tatctccatg
    37801 cattctcaaa attggtgaaa aagtagcata ttggaaccat cttaccataa gttctctaaa
    37861 ttctgggtct ttaggatttc ctccatggaa acctccaata tcagtagtcc accatggtat
    37921 acctgccagt cccatattta accctgtatt tacttgatta ttaaaagctt caaaactaga
    37981 atcaatgtca ccagaccata ctaaagctcc atacttttga gctcctgccc aagctcctct
    38041 tacaagagtt acaacagact ctatgtcatt atgtaaacca tcataaatca tttttaaata
    38101 atcaatagga taaatattac cacaacttaa aacatttcct tttttatatc tatagttatc
    38161 aaaatcatac acagaatagc caggttcagc tacatctaac caataatagt caatcccaag
    38221 ttgtttataa tttttatcta ttctatccca aacatattct cttgcatttt catttgtcat
    38281 atctacaaag actgtatttc cttgaatttg cattgtcatt ctaacaccac gattaacatt
    38341 gactaaatag ccattttcta aataatcatt gtagttttct gcatcacttt gaactgtagg
    38401 ccatacagat actattggtt ctactgaata ctcttctttc aattttttaa ctaattcttt
    38461 aggatttttc caataatcta agtcaaattt atattcacct tgcttaggcc aatgaaaata
    38521 gtcaattgca attgatgaaa gcttaattcc tcttttagaa tattccttca caacatctaa
    38581 aacttcttct gaagttctat atcttagttt actttgccat agtcctaaaa ggttttcagg
    38641 catcatagga acagttccag taacttgaga ataattttta tttaactctt ttgggctttc
    38701 tccacaagta atccaataat ctataaaatt tgtagaaaac atcttccatt ctgtcatatt
    38761 tttagcaaaa gatacttttc caattcccgg attattccat aaaagtccat aacctagact
    38821 agaaatataa aaaggaactg atatttgaga atttctttgg gctaactcca aaacagtatt
    38881 ctttaaatct aaaaatttat gttgatattg tcccattcca aaaatctttt cagaaggctc
    38941 agattcaaat ctagtagtta cttcaaattc tccactatag cttggtcgat attctcttga
    39001 ttttaacttt agagtagagt taaagtcttt ggtaatctca attgtcccta catcttctcc
    39061 accatcatca tgttttacag ctcttaatcg aatatattct ttcaataata tttcattttt
    39121 atcattataa aaagtaattc tatctctatg gtctaagaca gcttttatct ttccattttt
    39181 aatgattgca cttccatctt catttttatt aattgtaata tcatctttat ttaacttagg
    39241 tttttcgtta agagcatagt tttcatcaac aaaattttgg tctacaaacg acctaatacg
    39301 taaagagttt tttccccatg cttctattcg caatagttca ttgtcaaatc ttttttcaat
    39361 atagtcttca gatattttta tcacggctac ctcctaaatc aatggtataa tattctatct
    39421 ttgtgtagct taagttaaat aatgtcgttt agtatcgtta tttataaatt aggttttata
    39481 tataattcat ttgtacatta aactaactta ccaataatat actttatttg actttgtttg
    39541 tcaatcgctt tccggatttt agtaaaattt tctaatatat tgctggaaag tcgttcttaa
    39601 tatcttttat ttatactttt aatatctgtg atgataccta tatacatcta gtttttttaa
    39661 aacacataca ggaataacat ttgcctatta tgtagataat atcggacttg aaaatatgta
    39721 tagtgacctt ataaatacag ataataatat attagatatt gttcaaaaca atggatttta
    39781 taattataaa taatttatta aattatttaa gaaaatatac aattgtatac caagtcaaaa
    39841 acgacaacaa ataaataaaa ctaaaaaaga tgttgtctca aaatatagat aaatttattt
    39901 ttcattgcta aaatgattaa aatatatttg atttaatata cttgatttaa tataaaaaaa
    39961 gagtgtctca taaactaaaa agttcacttt tagacactcc tttatatata ttatctactt
    40021 cttaattcat ttattacctc tctacatttt ctttcaacta gtggatacat ctttataagt
    40081 actaattact ccacttaaaa atgattcagg tactgtccat cagtcatatt tttctgccaa
    40141 atataatggt gtttcataat gagacaataa atatttatat ctctacttat cttctctaag
    40201 ctcgtcaaat ccaactatag gtatatttaa attatattct ttaagtagct tttctagctc
    40261 ttctataaaa atacctttcc catccaccat ttgtatatca tttattttat ccatcaattc
    40321 attgcaacca cttaactcac caaaccaacg tttagaacta catgtcttat tataaccaca
    40381 acttggactg ccatctattg caatgatact ttttatatca taatcatttt ttaaatattc
    40441 atcaaactgc cttacatgag gtattaacat ttgcctacat tgttctctaa aaaacaaatt
    40501 gtcaaactgt tcttttacat gtccccacct atttattcca tacataatca tttctgggca
    40561 tggtagttga attattccat accctttatt tattaaatct atcgctatat tttgtgctcc
    40621 cttaaattct gacaatccct ctactttaga attacaattt aatatacaat gacatactac
    40681 tacaatctct ctatttctct tcataataat ctccttagtt attcttaatt ttaatgatac
    40741 tacatatttt gataattata ttatttattt gttctatatt tccaatattc tatatagaaa
    40801 tatacaatta gtaatctttt ataatctata ctatgataaa tgtatatatt tttttaggag
    40861 gtattataat tggatactaa aaaagtcaca ttttcttcaa tgtgcatagt tattaatatt
    40921 gtcttaggta tggttatatc tatgataaat ataccattat tatttttaga tacaattggt
    40981 actgtacttg gggctgtcgt attaggtcct ttttggagtg ctcttattgg tgggtgtaca
    41041 aacttagttt taggtgttat atcaggtcct acaaatatac cttttgccct tgtaaatatt
    41101 gttcttggtc ttattgttgg atatatttct aagaaaaaag gttttggtta taaagaagct
    41161 attattacag gagtaatttt atctattgtt tgtcctttaa ttggtactcc tatttcagtt
    41221 ttattgtttg gtggtttaag tggaagtggt gctgatttat tggttggttt tttagttcag
    41281 tctggtcaga agatatttac tgctgcgttc tttccaagga ttcttagtaa tatagtagac
    41341 aaacctttgt cttgtattat ggtggtatat tttataagta aaatgcctaa aggttttata
    41401 aaacaatata gtaaaaatac ttctagttca tattaataaa tcatttatta tgtaagattt
    41461 attactcttt aaaatgctat atactcattt ttatataaca tttttattaa taatttaatt
    41521 agatagtatt tgtattattt taattaaacg taacctcttg atataaattt gagctataaa
    41581 tctatatcaa gaggtgctat tttattttta atataattaa tattctatta cgcaaaaaat
    41641 acatatattt cattatttta tggaaaatat tacttcattt aactctatat caaactcatt
    41701 aagtaaaaat ctaattatat ttaatttagc ctctgtattt aaatttgttt ctaaatatat
    41761 attttctgat attttaatac cttttcttaa tttatatgca tctctagact taatattatc
    41821 tataaaatct cttttataag tcttgtctac aatactgtta aaccttacag aatccaaatc
    41881 atataaagta agagcaactt gttcataaaa ttctgtccaa tttttagcct tatatgactc
    41941 tcctaatata ctaaatgata ctatactttc atttgtaaaa ttaacatcat catcctctaa
    42001 cgtaaatata ttttcactgt tacttggagg ttcatatgta acacttggat atttccaaat
    42061 ttctacagct ctatctagta atatcttagt tctatttttt atttcttctt cattccattt
    42121 atctatagat gaaatatatt tatttagata aagcctacta tctttaaatc catcttccat
    42181 ttctttcttt tcttcaaatg ttttattact tagttttgaa ttatatcctg tcaaagttaa
    42241 atttccaatt gtatgtaaat atttatcatg tatctcctta tagttatctc ctagtgattc
    42301 cttccattta tttgttaatg tctgtggcat tatatgctct atatttagca tattattatt
    42361 gattaaattt tctaaatcaa caatttcttt attattataa ttctctaacc tctcaagtat
    42421 atataactta ttctttgact tagtattata tatatcttta gtttcaaaag catttgaaaa
    42481 ttcactgtca gtaggaaatc tttgagatga tttcttattt ataaaaatat atcttaatat
    42541 attagaatag ttttctttat aatcactaaa ttttttaatt tcccttccta agttcataaa
    42601 tactttattc agtgcattag gaggtacatc acatactatt cttcttaata taaaacattc
    42661 tactaaatta agggattcta caagtacttt tttatttatt ataccttcat tataatcatc
    42721 aaataattct aatataaacg gatatgatac aaatatttca agtttgttta tccttttcaa
    42781 aactttgttt acatcattat catcagtaca tgaataagat atctctctat aatagttaga
    42841 aaatttaagt aaatctttta ataaaatttc tatatctata ccattatctg atacatacct
    42901 tttaaaacta aaatatactt tatttttatt cggtatactt ctctccttta tagtcaaata
    42961 gtctcttata aaactactta cgttgctact tgtatttttt tcaattttat accaataatt
    43021 attataaaat tcctcttgta acttagagct tctacccatt aacacaaagt ttcttactct
    43081 atcagcttca cttaaatcta agccagttga atttagactt tcaaagatta attgagggtc
    43141 atcttcacca ttttttaact ctatttctac tatagttaat ttctgtatag catcaaacaa
    43201 atcatctatt gatatattat cttcttttat tctattataa aaatataaat aatttgaaac
    43261 tatattagaa tttctatctg tattcatttc ttcttcaaat agttcatagt acgcttttct
    43321 atcttctgct attggtttta actttatttt tccatcttta tctcttgagt gcttgtttat
    43381 aagataacac tccttaatct gttcttctgt tgtaataatt ccttttgatt ccccaaactc
    43441 ttttattatt tgacacatgg caatcaataa tattgacaca gtagtcactc tttgttgtcc
    43501 atctattatt aaatattctt ttcccatact atatatagat acaactgacc ctaagaaatg
    43561 agttctaaat ccagaatcta ttatatcctc taaatcatca aacaatcttt tacattgttc
    43621 tttcttccag ttataatttc tttgatatac aggtactaca aaacttttgt ttgacccctc
    43681 taaataacta agaaattcaa tttctcctgc tttcatatta actcccccta taagttaaat
    43741 aaattccatt cataactcca tgtatctata tattatatca aaattttgac tcataaatgt
    43801 atatgaaagc cttattatat acctttttat acctgttttt attttattaa agtaaataca
    43861 tgattattct ataaaatata caaatactta attttaaaaa ttattattta atcttcttca
    43921 tgagtaattc atagttattt atcaattaaa aagagatatt gcataattat tcaatacctc
    43981 tctttgtata tttacattat ccatatctag ttatctattt caacccaata ttagactacc
    44041 tactaataaa tttattctct acatcattaa caataatttt gcactaaata acatcattag
    44101 cctatctctg atgcattttc tttaatttta tttatagttt taatcaattt atttctctct
    44161 tcaatatgat tttcaaataa atcaattata cttccaacag ttctaaatgg gtcttccatc
    44221 aacactttgt tatcctctat aaatccattc ttgactacat aatcgactat aagtttaaca
    44281 aaatgtattt gcttagtatt aaagctctta ttatttaaaa actctgaaaa agcttcattt
    44341 gcagcttctc tatccaatcc cactagtctt ctcactaatt ggcttactgg agtatctcca
    44401 aactccttct tataatcatc acaattacct aactgtttta agagtatttc ctccaacgtt
    44461 tcaacatctt gctttgttat ctttttatta tttcttaatt taaatattgc taattcatct
    44521 ttatggctat ttaaataata ctctaccttc tttttataat tcttaagatt gtttgcatta
    44581 tatacagaat catttctatc ttctgctaca atcatatctt caaaacttgt ataatatatc
    44641 ttttgactag acttttcaag atacttaata agctctctta atgatatacg tacttcttct
    44701 acctcaaata tatctgaatc ttcccaaaaa tcctctgtca ttgctttttc aatagtatat
    44761 ttttgttgct gaatctgtgg tatagtccca agttttgaaa gtttttctgc tgtagccatt
    44821 acatgcctta tacctttact tgcattatta ttttgtaagt atgcaagttg tatattatac
    44881 ataactacat caaatctttt agccaattca tcatcactat atgaagttat gagtggtgat
    44941 atatattctc ttatctcatt ttgtgctact gctccaatat tgctccaaac attttcattt
    45001 ttatacttat gtacataact taaattcatc ctaactatat aactatcctc atttaaagct
    45061 tttacagaag ttataagttc ttctaacaat tcttttctaa gttctgcata ctcaccttct
    45121 atatatttaa tatcttgaag ttctttaacc atacttacct ttatattaaa taatttttca
    45181 gtaagtgttt ctgttttatt tccctcaaat cctttaggat taaatttaaa aaattcaaaa
    45241 ttattacaaa aatcaaatat taagaatctt tccttatcca tatcaacacc taataaatct
    45301 ggacataatc tagttcctct acctatcatt tgccaaaact tagtcttact tctcactttc
    45361 ttgaaaaaaa caagatttag tatttcaggt atgtcaattc ctgtatcaag catatctact
    45421 gaaactgcta tttgaggtaa tttattctta tcagaaaaat catctattat agaatctaca
    45481 taatttatac tatagtcaac tgcttttaca aagttcccac catactttgg atacagttta
    45541 ttaaatcttt caactattgc cctagcatgc tttgtattct ttgcaaaaat tatagtctta
    45601 ccaatctttt cttctccttc tattttcaac ccctctgtca ttagcttatt taaaactaaa
    45661 tctattgtgt cagaattaaa tagccactca tttactgcat tattccctat ttcgtctcca
    45721 atactctcat catcattaaa tgtattttca tattcttcct tctcttcatc agaaagctca
    45781 tcatacttaa ttccatcttc cataatctta gtcttaaact caatactagt ataatctact
    45841 aaatatccat cctcgactgc cttattatac tcataagcat atgtaggtac tccattttcc
    45901 atgtcaaaaa ctgaataagt atttttatct atctcatcct ttggtgtagc agtaagaccc
    45961 attaagtaag aatcaaaata atcaaagatt gacttatact ttttataaat acttctatgc
    46021 gattcatcta ttataattaa atcaaaatga ccaggtgtaa acaatctctt tccatcctta
    46081 gacttcgtat catcaattgc attcatcatt gttggatatg tagaaaatat catcctagca
    46141 tcttctgggc tgtctttgct atccaataaa ttacataacg ccaagttagg taatagctta
    46201 gtaaaacttt ttttagcttg ctttaccaat gcttttctat ctgctagaaa taatatattt
    46261 ttaatccaat tatgtcttga aagtacatca actatagata tagcaattct tgtttttcct
    46321 gtaccagtag cacataccaa aagagttttg cgttgcttat tttcaagagc ctcacataca
    46381 gccctaatag cttctttttg ataatatcta ttggatattt catcatttat tgttacagaa
    46441 cttaaagact tcttactctt tcttctatct atcataagtt gtaattctga ttttttataa
    46501 aatccataaa ctttcctatc tgaatagtca tcccaaatat acatatcaaa accattagtc
    46561 aaaaatatta ccggtctttg actatatctc ttttccaaac aatctgcata taatttagct
    46621 tgttgttgac caattttcgg gtctttactc gttcttttcg cctcaactac tgctaatggc
    46681 ttaccatttt caccataaag aacataatct acatatccca caccactttc attaggcata
    46741 ccttgtactt ctatttcttc ccatatatct tttccaaatt cccaacctgc aagctttaat
    46801 tctatattta tatatctttt tcttgtctca aattcgctta cttcgtctat cttgaaatca
    46861 tagtttacag tattaacttt tctcttttca gttagtgact ttcttaattc ctcattttct
    46921 tttattactt cttcaagctt tttatctttt gaacttaatc tctcataaag atttttaagt
    46981 tcgtctggtc tcgtcctttt ctcttcccca tgaagtaata aatcttcatt aaagtcttct
    47041 gctgtatatt catctgaata acaatagtct atccaactaa caaattgatg caaattgtta
    47101 agtgaaagta ttgcttctcc tctctctatg ttcgcactag tatgtacaga taaattacct
    47161 aatttaacta tatattttaa taaaggtaac atatcatagt ctataagttc tataaaacta
    47221 ttattatgaa tcaacgtact aagattatct tgataaggca atactaaatc actatcatta
    47281 gcatatagcc attttactgc aagctctaaa gctcttcttg ttatagttgc acaagtagat
    47341 gggctaacta gtatactttt ttctgcttct aagcaagcag tattaaaact attgaaattt
    47401 ttcttctttt gtaaaaaatc aaaattaccc aaggctttta tccccctttt gtcccttagt
    47461 taaaataaat ctctctaaaa actatttctt acagctgttt cttatactaa caaatacttc
    47521 ttcattttct gtaaacaatt cgtacatatc ttcaaactgt ggtatatcat atccatgaga
    47581 atcatcattt ctcaattttc taagtgtttc tataactata gaagaatttt taggtaataa
    47641 attgtgatac tcacaagcta aaaggtagtc ataaaagttt ttagatttta tgtttaaatc
    47701 aattgattta taatacaaca ttgcttttgt taattctaga aatgatacca tatagctttt
    47761 aaagccttct tttatgtatc tagcccataa ctcaggcatt tcttccttat catataagat
    47821 tttgaagttt tgcaggtcat acaagcaaga accaaaatcc ctatttactt cttctattga
    47881 gttccaagat aatttgtttc taattaccat acatatccct ccaatctaag atatctatta
    47941 catcactgtt ttcaaaatag aatttattta tataatcctt aaaatcttta gatttataaa
    48001 taacttcacc ttctaaaata gcttttttaa gtgctattgt ataattatct gtaacaattt
    48061 ttaggtctac atcaatgtct aatatatttc ttagagcttc tgtcttagtt gcaagttctg
    48121 taattggtac ttttttgatg ggaatccaag ctatgtctat atcactttct gatgtatgtt
    48181 tatctgttcc ataactacca aatattatga ttgctagagt attttctttt ggaaatacat
    48241 ttattattga gtttttaatc tcatttggta tatttctcat ttaatcacct cttgtttatt
    48301 aggtaatatt gcttaattga atttatatcc ataatctttt aatctattta tacatatttc
    48361 tctttcttca tcagtaaaca actctttata cttttctttt aatactaata cttctaatga
    48421 taaatctaat ctcttacact cccatagcct tgtaaatcct tcagtagctc cttctttatt
    48481 gattaattct ttagcagcta ataaggctcc tttgttattt atcagttgta aaaatcttgt
    48541 tggcttatag ttacattgta aatcagcttc tttatagata tttaccatgt tatggtataa
    48601 atctagctct atttgattca tagattttat tcccccattt taataattgt attttagttg
    48661 aataactatc ctttaaaagc tttttgcatt aatgagttaa agttgtcttc tagttctttt
    48721 agacttgttt ttattcctac tttcaatttg tctacttgat taacaaactc ttcaaactta
    48781 ttttgaagtt ctattgatgg tactggtatt aatatttttt gaagttcttt gcttctaatt
    48841 ccttgtgcag agctaccagt tttatattta tctatttcta ccctaaaagc ttttgtttgc
    48901 atatattgtg ctaaaaaagc attatttaaa atttttttat ctccctgaat tgttataatc
    48961 ctttgaccca tagcaaactt ttttaaatcc tttggtatcc tacatacatt tccaaatgta
    49021 tgaccttctg taacaaaaag tacatctcca gctactggaa aacctctttt catccaagaa
    49081 ttataggtgt tttcagatat atattcatta atatatttaa atacaccatt ccctacagat
    49141 tttgcattta ttattctaat tccattctct gaaagaattg gagttttacc acgataatca
    49201 ataaaatttg tacaatcttt caataataca aacttttgtt ccttaaaatt tccaaacatt
    49261 tcaataaatc ttgatttaac taattcatcc aatgcttcta tttgctcttt tcttttatct
    49321 attaactctt gagctttatc taaaactttt gatatatctt tttgagcttc catttcaggt
    49381 aaatctactt ttaaattttt taaatcatta actgaaaaac ttgcttgatt aactgatttt
    49441 tttattatcc ttggtaactg tcttctaaat tcattactat taaaaaaata atttaaatac
    49501 tttggataac aaacttcttt atctggtcgt aaacataaaa gattcattcc atgtataatt
    49561 tttttattta tatcttcata tattgcactt ttccctaaat gtttttcact attaatatgt
    49621 gacattagta tatctccatt tttcaataaa tagtcttcta attttcctaa ctcttttatg
    49681 tcagcatatc ccattttatt agtatcaata taagagtttg ctatagtttc tattcttgta
    49741 attggatatc caaattttgt atcaaattgt ttaatactag ccccatttct aataaattca
    49801 aaaacttcat tcaatctata ttccatactc tacacctcaa gcattctttc taattcctct
    49861 aatccatcta cgatttcctt ctctaactcc ctaactctac ctataataac actaggagca
    49921 tcataaacta cttcctcata cactacctct ttatacttat taatagataa atcataccca
    49981 ttctctctta tctcttccac atcaacaaaa aagctttgct ctgttctttt tctatctact
    50041 tcaccatcaa gattatcaaa tctcttaata atatcctcta tatcactatc ctttatctca
    50101 tttctcttat catccaaaga ataaccatca gctttcatat catagaacca taccttatca
    50161 gttccacctg ttcctgtttt agtaaatatc attatagctg tagatactcc tgcataaggc
    50221 ttaaacactc cacttggcat tgatattata gcttccaatt tatgattatc aactatttct
    50281 tttcttatcg atttatgtcc acctgttgaa ccaaacaata ctccatctgg cactatacat
    50341 gcgcatcttc caccaatttt taaaactctt aaaaaaagtg ccaagaataa taactctgtt
    50401 ttctttgtct tagtcacttt taataaatct gctgacactg cttcataatc taaacttccc
    50461 ttaaaaggag gatttgctaa caccaatgta aacttatctt tatctgtatt aacctccgat
    50521 aaagaatcct tatactctat attaggattg tcaacgccat gcaacatcat attcatagca
    50581 cctattctaa gcatagttct atccatatca aatccataga acataccatt attaaaatgc
    50641 tccttcagcc cttgcactaa gaaaagactt gaatgattat cccttaagta ttgttgtgat
    50701 gatactaaaa aacctgccga acctgctgcc gggtctacta taatatcttc tggtgttggt
    50761 ttaataagtc ttactatcat atctattatg tgtcttggag tcctaaattg accattagta
    50821 cctgctgtag ccactttgga taataaatac tcatacaaat ctccctttgt atctctatcc
    50881 ttacttaatt ctaaattact aataccatca actattttag ataacataag aggcgtagga
    50941 attttaaata ttgcatctcc catgtacttt gcataagctg attctttatt tccatgtaat
    51001 ttttttatga aagggaacac ttcattttgt actatttcat acattttttc tgaatttcct
    51061 tcattagaaa acttactcca ccttaaatgt tgatgttcat catcaaatat cttttcaaaa
    51121 gaaacaccca aaagagttgc ttctgcttca ttttttgttt ctacttcatc caatccttta
    51181 ataaataaaa gatatgtaaa ttgttctata acttctaatg gattagttaa tccaccagtc
    51241 caaaaagtct cccaaatctt gtccacttta ctccttaatt ctcctgtaat catatatgac
    51301 tttccctcca gtgttcataa aataatttaa atatatattt tagtcgtacg ccttaatagt
    51361 tagtgtctta tgttaaatcc aagtttttac tacacaatta ttaaattatt tttcctattt
    51421 acatatttta gctaaaaaac taatgcaata ctcttatttt atgattatta tatataccat
    51481 acaaaattat tttatcatat atccatcata gaagtcatct acctggataa ctcttaccat
    51541 attataactt tttaatatta atttaaactt agaaagaaat tttttattta attatatcat
    51601 cttccttatt tcaaatttct caattacaat cttaatcaaa aaataaaata agatgctgtc
    51661 tcaaaataga tataatcttc ttttttattg tcggaatgat gaaaatatat ttaatttcat
    51721 acaaaaaaga gtgcctcatg aactaaaagt tcattttgag acacccctta atttaatctt
    51781 acttattctt tccctttatt attaacactt cactatctct attgcttttc ctacacatcc
    51841 acaaacttcc atcctactct taacaaaatc tttaactgac tctaaacctt ttttaccata
    51901 ttttttaggg tcaattacct ctgggtcaga attaagcaca tccttaaccc cattgctata
    51961 agcaatcctt aattcagttg cataattaac cttgcaaatc cctcttttta tagactcctt
    52021 aataacctca tctggtattc cagacccacc atgtaatact aatggaactg atacaacctc
    52081 tctaatttca gataatctat ctaaatcaag ttttggctct cccttatata gcccatgtgc
    52141 cgtacctatt gctactgcta aagatgatac ccctgttctc tctacaaact cttttgcttc
    52201 taatgggtct gtatatgctc caccatcacc accatctaaa tcgtcttcct tacctccaac
    52261 tttacctaat tcagcttcaa caggtatatc tgaaggcata catgcatcta caacagattt
    52321 agatattgaa atattatctt cataactttc atgagaacca tcaatcataa tagaagtata
    52381 tccaactcta agtgcctgca tagctagtcc aaagcttgaa ccatggtcta agtgcattgc
    52441 tactggaaca cttgccttct ctgatgctac ctttacattc gctaaataat aatctagtcc
    52501 tgcatacttt attgttgaag gtgttgtttg caatattact ggtgatttaa gttcttcagc
    52561 agcctctatt actgccataa ccatctccat attttcaaca ttaaatgcac ctactgcata
    52621 atgcccttct tgtgcatcta ataatatttg tttagttgtc actaatgcca taatttctcc
    52681 tcctatatgt ttatactgat ttaatttctt cgttatttaa ttttgatact atttctctac
    52741 ttgttttgct atttaataac tcatttaaaa attcttcatt cattaatttt gaagatagtt
    52801 gagataatgt ttttaagtgt aaattatcac cattatctgt agctgctatc ataaatataa
    52861 tatgtgccaa gcttccatct tctgattcaa agtcaaaccc ttcttttgat agacctactg
    52921 ctacacgtgg tacctttact gcatctgttt ttgcatgtgg tatcgctatc cccataccaa
    52981 aacctgtaga tgatatttct tctcttttaa ggatttcttc tttaaacttc tttttatcta
    53041 aaagaacact agatttatca agtattccaa tcatttcatc aattacatct gttttatttt
    53101 ttcccttaac atctaaaatt actgtttctt cgctatataa agagatatta ttagatacgt
    53161 tttccgattc tgcactttca tttttatgtt catcttttgt tacatttgta ttttctatag
    53221 tatttttctc atttactact ttaggtgtaa ccatcatata aagtacagca cctactaatg
    53281 aacctacaaa tatacaccct acccataaaa gtggcttatt gactaatcct agaaccaaaa
    53341 accctccatg tggagctggt acttctattt tcattatata agttaaaatt gatgatattg
    53401 atgatgcaat cataagtact ggcaacactt ttaatggatt ttttgctgca aaaggtattg
    53461 ccccctctgt tatatgagta ctacctagta tataattgat taatcctgct gctctatctt
    53521 cttctgtaaa tctattttta aataaagtag atgctattgc tattaccaat ggtggtgtta
    53581 tacaagctgc agatacacct gccataaata aataatttcc ctgtcctaga agcatagttc
    53641 cagttacata tgctgcttta tttactggac ctcccatatc aaatgctgac atacatccta
    53701 ttactattcc caaaattata gggcttgaat tttgtaaccc cgatagaaag ttcatcatag
    53761 cattgttaag tgctgaacat ggcccaccaa gtaacaacat tgctacaccc acaagaccta
    53821 cgctgacaat aggaattata aaaatcgatt taagaccttc taattgtcgt ggtaattttc
    53881 ttaacaaatg tacaaatagt ttaactaagt aacctgctaa aaatcctgat atgattccac
    53941 ctaaaaaccc cgaacctgta tcaaatgata tcattcctcc aacaagtcct gccaacatac
    54001 cagctcttcc cgatatagat tgagctataa atgctgccaa tacaggaacc atcaagttca
    54061 tagaatatcc accaacagat tttaatgtag ctgctatttg attatattgt tctgaatttg
    54121 ggtcaaatga ataaatcccc cataaaaatg atatagcagt aagtactcca cctgctacta
    54181 ctaaaggtaa catgtgagat acaccattca ttaagtcttt atacaacttt tgaccaaacg
    54241 ataaatttcc ttcttctgca attttactgc ttgataagtt tccttttcta atagctactt
    54301 ttccatcaat acatctttgt ataagttcat tagctttatg tataccttct ttgacaggta
    54361 cttctattac tggctttcca ttgaatctgt ccatatcaac atttttatca caagcaacta
    54421 ttattgcatc tgccatttcg atatcttttg ctgttaactt attttctaca cctatagctc
    54481 cattcgtttc aacttttatg tcacacccta attccctggc tgaatttttt aatgcttctt
    54541 ctgccataaa agtatgagct attccagttg gacaacctgt tacccctaga atcattttct
    54601 ttcccatttt tataccacct ttctgactct tatatttttt atataatctt ccacattctc
    54661 caaattacct ataccattac tttcagccac acttgctcct gttgctgctg acctttttaa
    54721 tgcttcttca atatgttctg gattttccaa ccatatactt aaaaaagcag ctaatgcaga
    54781 atcaccagca catgcagagc tgactactgt aactggttga gcactagcat aataaattga
    54841 ttttccatta taaaaataag aacctttttc tcctaaagtt agcaatatat tttgagcacc
    54901 ttttaaatgt agtaatttaa gtacatcctt aatatcttct tcatctctta caataattcc
    54961 aaatatatct ttgatttctt catcatttgg ttttatcaaa tatggattat actgtagtaa
    55021 ctcctttaat tttggtgaac ttatatcgag tattgtcttt atacttttat tttcacaaat
    55081 tttaaatata tcctcatagt aattatcatc aataccagga ggtagacttc cacttattga
    55141 taagtaactt atatcttcta atgactgtat cttatctagc atatctagtt tttgtttttc
    55201 gtttacatat gacccagagt ttacaaattt aaattcatca cttccatcat ttaaaaatat
    55261 atttatacga gttgtatctt ctacccaagt aggcaatacg tcaaatcctc tcttttcgct
    55321 ttcttcaact atatattttc ctgaaaatcc tccaaaaaat cctaatatct tagattctat
    55381 accataatga cctagtacaa aagttacatt taatcccttt ccattaggag aatatacagc
    55441 atttgatgta cgatttacta attttctctt tatcccatta gtagatatat tcatatctat
    55501 tgcaggattt gtagttaatg tatatatcat tgtcttcccc cttgcttgtg ctgtgtatgt
    55561 tttcattata ataaaaaagt ctatgaaaat cttttcacaa atagaaaatc gattttcata
    55621 gacttttcaa tttaattttc agaattatta tatctgacca tataatctat aattatccta
    55681 gatataatat acaagggcaa tctggaagat acttcatatt cagaaattga tatatgttca
    55741 tgtttgtatc ctacaatata taaatccgaa tactttataa gttttgcact ttcactacaa
    55801 cagcaagtta taaccttagc tccaactaaa ttggcattct gtgctgattc tacaagctct
    55861 attgtatttc cattcaatga aaatataatt ataagttcgt ctttttttat tgatttagat
    55921 tttattttca ttatgttggg gtcatttata aaaacaacat taaaatcaag taattgcaat
    55981 ttaagactaa actcttttgc tacatattca gtaagccctc ttgctaaaat atatatctta
    56041 ctagatttat ttacagtttt tattatattg attagtgtag ttaaagatat ttgttctata
    56101 actctctgtg cttctataaa ggatttattt attatatccc ccatattttg tatatcatca
    56161 ttttcatctt ttactgttaa tctataccta agttcattga atccatttaa accacatttt
    56221 ctaattgctc ttgaaacggt tgctggagat gaatatgttt catgagcaat ctctactata
    56281 gataattctg gtaatattga ttcattttca tttataaatt taattatttc aagttccgtc
    56341 ttggttagat tttcagaatt acttttgttt agctcaatta tcatatgtta tctcccctta
    56401 tataagtttt atttgtctaa atttatcgag taatataata tattacctct taactactta
    56461 tttttatata caattaagat ttagtcaatg tctatatatg taaatttata atataataat
    56521 tttttgctta aagtttatta tgttatctta tagcatttta aaataaaaaa gaattgtacc
    56581 tctaccaaaa tttggatgaa gtgcaataga tattccttat actgatatgt ttatgattaa
    56641 aagtataaat ataaatttcg ctatgaatca tataattaat aaatcatttt atttacaatt
    56701 ataacccccc ttataaaact atattaataa tatatacagt atttataagg ggggttgttt
    56761 atatttaaaa actatttact tttcttttaa ttcttctcca ttagttctta ttacattttg
    56821 ataccaataa aaactatctt ttttataacg ttttttactt ccatttccat tgttatctat
    56881 atctacatat ataaatccat atcttttttc catttcttga gatgaacaac ttactatatc
    56941 tattggtgac cacatacaat aagctaatat ttcggttcca tcagctatag cttcacctat
    57001 ttgttctata tgttttccta aataatctat tctgtaacta tcatgaactt ttccatcttc
    57061 aaatttatca tgcattccaa atccattttc agctatcatt attggctttt ggtatctatc
    57121 ataatattga gataaagtat tgtataatcc ttttgggtca attgcccacc cccaaggatt
    57181 tgcttttaaa tatggattct ttatattgtc agttggattc atggtgtttt tacttgctga
    57241 tactgttgta gaataatagt atgataaagc tagaaaatcc atagtatttt cttttaagat
    57301 tttatcatca ccttcctcca taactatatt tatgttattt tctttgaaaa atctcttaat
    57361 atagactgga tactctcccc taaaacttac atcagtgaaa aaatattgta gcctatttct
    57421 ttttaaagca aatactacat tttcagggtc acaatcttct ggataagatg tacaatctgc
    57481 catcatcata cctatcatca tatctgaatt tatttctttt ccaagcttct tagtcaaagc
    57541 tgtagctatg aattgatgat gaatagcttg atatctagtc tcttctacat tatctacttg
    57601 gtctacacaa aatgcaactg aattaaatga ttcataatgt attaaattta tttgatttat
    57661 aactatccaa tatttaactt tatttttata tctatttagt attaattctg catatctaac
    57721 aaagaaatct accacttttc tattagacca tcctttatat tttaatacta aattcaaagg
    57781 agtctcatag tgaagtagtg ttacaatagg ttccatacca ttatctatta cttcatttat
    57841 aagattatca taaaacttta atccctcttc attaggctct aaatcatctc catttggaaa
    57901 tattctagcc caatttattg aaaatctaaa acactttaac cctagctctt tcaaaagctt
    57961 taaatcttct ttgtatgtat gataaaagtc tattccacta cgcttaggat aatatccatt
    58021 attatctttc atagcaaact ttattctttc tatagtcata tcagactcat aaggtttaga
    58081 tatatctcta tctttatcat acatatgtac atcagctaaa caccaacctt tgccaccttc
    58141 attccatgca ccttctaatt gattagctgc tacagctcct ccccataaaa aacctttagg
    58201 aaacttaccc aatttaatca ctccttattt tatttattta aatcatttac tattatttac
    58261 cttatattct atagacttta ctattcctac taaagacata gttatattta actcgctgaa
    58321 tatggtcata agagtatctt gagcatgtgc aaataacagc gaatgtgctc ttttctctcc
    58381 accagcttcc ccttgtataa tcttggtttg agctctatgt gcttctgtca taaacccttt
    58441 tgctttagct agttttaaat cagcatttct ataatcaaac ttttccaaat cattaagagc
    58501 atgtttaatt tctgttctag cctcacctgc acgaagtatt atttgcattg ccacctctac
    58561 taattcttca tctattatat gttcttcaat tgtcatattt ataatttcct ttcaatttct
    58621 aaatttaaac ctcttggctc tctaatttta tctcttcttt ctcataagct ttaaaaaatg
    58681 gataccatat cataaatagc aatactatat ttattgctaa aacaataaga cctctaacat
    58741 cttgatttac cgtaaaagta gatataccaa aaggaacata ccataaatta aacaccttac
    58801 ttggaattgt cacaaatccc atactcattg ataagtaagt agttattgat attactactg
    58861 ttcctatcca cataggtaac attaaaaatg ggttccatgc cactggagct ccaaatatta
    58921 ctggttcatt tatattcatt atagaaggga caatacatgc tcttcctatt gcttttattc
    58981 ttccaactct ggataaacac atcattataa ctaatggtaa tgtagcaccc aaccctccta
    59041 aagttatcca acctgtttga ataacttcgt aaacatatat attagttgct actcctcctg
    59101 atgctactgc tgctgcatta gctgcaattg caccaagttg aattgaataa gttataggag
    59161 atagtaccca cccacttatt cctaaagaat atataaatac tactaaaaag tttaagagta
    59221 caaatccagg taatgtctgt gctatatctg atactggttt aaatactcta actattattt
    59281 caaacatgtc aaagttgagt ccataaatta atccccagcc tacaaacatt atcactgtta
    59341 taggtaacat attatcaaac cacttgctta caaaatccgg aagtgctgaa tcttctccga
    59401 aaaatgaaaa agaaccaaat aaggacatta ttaatccaga gaataatcct gcaactagcg
    59461 atacaagcat tccttctcct ccaaacctgt tgaagttaaa tatagctcct tcattcgtaa
    59521 tctctggacg aatcatcatt aacattaatc cagcacctgc aagaccagcg actacactca
    59581 tactttcaaa tttcttattc ttcattattt ccataggcaa taaaaatgct ataaataatc
    59641 ctaaaagtcc aaatgtatat tgatttattg gacttaaatc aggtaaaaag cttatgtaat
    59701 tcttcaagac agaaactata gttactaatg agcctacaaa taccattggc aaaactttca
    59761 ttatagttga ttgaactgat tgtacccata tattcttagt catattattt acaacaggag
    59821 taaatttttc acttaaaaaa tctataaatt tattcataaa cactctcccc ataaatattt
    59881 tatttgtttg atattagttc tagtgcttct tccaaagcct ttgccccatc tagtcttcca
    59941 tatgcagttt tagatataca tgctactgga acgttatatt cattagcctt ttgttgaatt
    60001 tcttccttca tataatctaa atgtggtcct agtaataaac aatctatttc tgataaatac
    60061 ttatctatct ctgactcact tctcgctttt atatctgctt ctataccttt tttctttgca
    60121 ctctttctca tactagttgc cataaaacct gtacttgctc ctgaaccaca cgccaaaagc
    60181 actcttatat ttttcataat gatacctcca atatttttat ccatcaattt atttcttaat
    60241 gaacttttta ttttattatg tttatattgt acttaccaaa taagttcatt tcacataaat
    60301 atatttccta ataataggaa atcagttact cttaattaaa catattaata tagtataatg
    60361 tatcttataa tacgtacatt caattatata attatcatat catccagatt ttattttaaa
    60421 actatgcgag ggaatctaat gaaaacatct aaaaaagaac tatttttaaa tttcataaaa
    60481 acacaacatg attggataga ttcatctaca ttggctaatt atcttaatgt ctcaacgcgt
    60541 tcaattagaa aatatgtaaa cgaaattaat tccaatggtg aatttatatt atcctctaaa
    60601 aaaggatata aagtaaatct caataataat tgtcaaacca aggttgatag ttcagaaaat
    60661 atatcacctg ataacaggtt aaatttaatt ttaaaagaac ttatagttaa tagtaatgga
    60721 attaatatat ttgatttaag tgaagacttg tttgttagtc cagctactat tgaaggagat
    60781 atagtcaaag ctaataaatt tataggaagt tataatttaa agataaagca atcgaaattt
    60841 ttattgaaac taattggaaa tgagtctgca aagcgcaaat taatgagctc tataatattt
    60901 aaagaaacgg gaagtgattt tttaagctta tttgatgtac aaaaaatata tcaagagtat
    60961 aatttgacca aactgaaaga aaatataata tatatattaa aaaaatacaa tttgtttata
    61021 aatgagtatg ctataaataa catacttctt catttaatga taactataga cagaataaaa
    61081 aagaataact acatagaatc tgtcgaagta gtaaactaca tagataataa taatgatata
    61141 agcatttcaa gagatatatc aaatttttta gagtctgaat acaatataac tttaactagt
    61201 gcagaattat actacttagt atttcagtta acaaacaaaa ccacagtctt aaattatcat
    61261 gaaatggata ctaagtcatt gccaaattat atagatgaac attttgttaa attaactaaa
    61321 aaaataatta aaaatgtata tgacttatac tttatagatt tatctgatga tgaatttgtt
    61381 gtaaaattta ctcttcatgt aaaaaattta atcagtaggg ctaaaaataa tcaagttttg
    61441 agaaatcaaa tacctcagaa gttaaaagat tcctaccctc tgatatatga tatatctgtt
    61501 tatatttgta atcagattca aattttagaa aacatagaaa tagatgaaga tgaaatatcg
    61561 tatatatctc ttcatgtagg ttcatttttt gatagacaaa aattactaga agataaaatt
    61621 ctctgtgcat taattactcc aaattactat gatttacaat tcaaaatagt aagagacctt
    61681 gagaagagat ttaatgaatc catagaaatt attcagattt tttcagatac tcataatctt
    61741 gattttgaca ataaggtaga catggttatt actactttac caattagcga tagatgccct
    61801 ataccttttg tttatgtaaa tccttaccta aatagaaagg attatgataa tattcaaagt
    61861 aaatttattc agattaaaga tagaaaaaat atacttactg tacaaaatca tttagaaatg
    61921 tactttagtg aatctttgtt tatgaaaaat atatatttag atagtgctaa agattatata
    61981 agatttatgg gaaatattct gtacgaaaat aaatatgtca aacccaatta tatagatgat
    62041 gttcttataa gagaaaaaat gtcttcaact gcatttaata acaatgtagc aattcctcat
    62101 agcatgaaaa tggatgcttt aaaaaccgga gtatgtttaa ttgttaacga taaaccagtt
    62161 aaatggggtg aggaaaaagt tcaaattata gctatgatac ctataaatga aaaagaaaaa
    62221 gaaaagttca attacatatt tgaaagcttt attgaaatat tatctgagtg gaataatgtt
    62281 aaagaactta ctaaggcaga taactatagt agttttatga atagaatagc ttatcttatt
    62341 caaaatatct aaatcaatta atttatttcc aatatatgtt tcttaagtta ttaacataaa
    62401 atagataagc tgtcacacta aaaagtctaa attaatatgt gacagctttt tacctaatat
    62461 accttgtttt aatactacat attttttgac tgttgtatac tagttataag attgaatatt
    62521 tgtgttgttg tagctgtcat attctcacta gcagttgctt tatccattgc ttcttctatt
    62581 gtcatagctc tatttactat agaaaaataa gcatctatac cctcttcatt acatttaact
    62641 gcatcttctg ttgtggctcc agccaaacct attactttta ccccatattt ttttgccagt
    62701 tttgcaactc ctattggagc cttgcccata gcagtttgat tgtccaatct accttcacca
    62761 gttatgacaa aatctgcatc ttttaactct tcctctaatt taatttcttc taatattatt
    62821 tttattccag attctaattt tgaatttaaa aatgctaaaa aagcaaaacc taatcctcct
    62881 gctgctcctg ctccttctac attagctaca tctttactta agtaattttt aactactttg
    62941 gaaaaatttc taagcccatt atctaactcc tcaattattt ctgatgttgc acctttttga
    63001 gacccgtata tgtatgcagc tccatttttc ccaaacagcg gattatttac atcacaggct
    63061 attttaaact tacattcatc taattccttt aatctatttt ctatttttat atgtcttatt
    63121 tcatttaaaa cttttccacc taatcctact aacttgttat tttcatcata aaactcaaac
    63181 cctaaggctt gaagcatacc tatcccagca tcattagtag cacttccacc tatcccaact
    63241 ataaaatttc tatagccttt atttattgct tcttttatta tttccccaac accataagtt
    63301 gtagtgtata aaggatttct aagttcagtc ggaaccaatg ttagtcctga tgcttgagct
    63361 atttctatta ttgctgtact tgtatttttt aataatccat atgttgcatc cactttttta
    63421 gctattggtc cagtaacttc tatagtatct tttttaccat tcatcccttg aactaaggca
    63481 tctactgtcc cttcaccacc atcagcaagt ggcaatatct ttacttgcgc atcttcttta
    63541 accttcaata tccccttttt tattgcattt cctgcctcta ttgaagataa gcttcctttc
    63601 aaagaatcta tagaaattaa tattttcata tttatctact cctttttatt actccttatt
    63661 ttaaacatat aattttataa gcttaattta tcttttattt tatactctac atctctcttt
    63721 gtatacaact tataccatta atattctttt atcttataaa aagggagtgt ctcaaaataa
    63781 atttcttagt tcatgagaca ctctttttgt ataaaatcaa atatatgttt attatttcaa
    63841 caatgataag gaggattatc tctattttaa gataattcct ttttagcact atttaaccag
    63901 tatagtttta acttggttca ttaagcactc tacatttaca gagttattat ctattgatat
    63961 agattctaat tctttttcat ttaggttaat ccatttagct gttttaatat ttttgttaaa
    64021 tactatgtta gctgattttg tttcttcttt agaattgtaa aatctaacta ctaatgcctc
    64081 ttgtttttca gcctttttta caacacttaa cacaaggtcc ttattagttt ccttaagtaa
    64141 actatatgaa taatctactt ctatttctga ttgattcaat ttcatagcat tatatggcat
    64201 tttattataa gtttgtattt ttgttaaata ctctttagcc atatttgcaa catttgcttc
    64261 tagtgtagat ttttcatgag taactattgc taagtctatt tttatatctc ctatcatttg
    64321 agaatctgga gtaggcaact ttattcctga tggacgacct gggcgtcttg ccatttcttc
    64381 tttacctagg aatcctacac ttctaaataa tgttatagct attgtgtcaa atttttcccc
    64441 tactatttca aactctcttg tactgtttgt aagaacagct actccatgct ctttgtcaga
    64501 caatccaacg aatgttagca ttggatatat agaatctggt ctttcactcc attcttcctg
    64561 ttgccaaact tccatagcat catcatacac acttctcttt atatgtccaa actgattatc
    64621 agacacagaa aactttgaag ctatatttgt tggtatataa gttctaaccc tacaatctct
    64681 tgcttggtta tttatgttga atactacatc tattatagct ttatcatttg gaacatttat
    64741 aactatatca aagtcaataa atgaatctgc tctattattt tttctagctt gtatatcttt
    64801 tggtactttt aatctataat ttatctttat ctcattatta aatctatttt tctttatagt
    64861 ggaatttgct actatattat cgttaaatat taatttctca tttggaagtg gtgaaaaatc
    64921 atactcatct ccttcatctc ctccattttc catcaataat acattttcaa aagttttatt
    64981 taatttttta tcaaatatct ttaatgtccc gttttcatta acttgaatag tataaaagtc
    65041 attatctacc atgtcacata cttcataagc tttttctaac atatgttcaa cttcttttat
    65101 catatacgcc ttatacccca ttgcaggtat tttatcttta aagtttattg tatataccat
    65161 aaatgaatca tagtttccat aatgaacaat ttgacggtct attaatcctg catctattac
    65221 ttctttatgt accacttcaa aatccaactt attattatct ttatcaaata tttcaaatgc
    65281 tttcatttta gttataactt gagcgcttac tatttcattc ctatcgtaag gcattaagtt
    65341 aaatgctact aacttatcta aagccatttc acaactcatt gcatcaacta ttcttctttt
    65401 gtaaaatgtt attaattggt ctactttttc ctcagctaag aaaaatctat tcattatttc
    65461 atggtgaact ttatctgaac aacaacatcc aatgctgtca tgagcatgat ttttcattat
    65521 ttctttccaa attaactcta ttaatccatg atgatattca aatcctagag aatatgcaat
    65581 agaagctagt ggctccaata tgttggttat ctttgattct attctagcat ttgctgattt
    65641 tatatccatt cttgttgaga atatacttct atgaaccctc atatatttcc catctaaaaa
    65701 ttcccctttt attgtatcta tatcctcatt ttttctagct ctttaaatat gttttcatat
    65761 ctacttaaga aaaattttct ctctggataa cattctttta atttatatat aacttcaaat
    65821 atattctttt gaattggcat ttggtcatgt ccatttggaa gtattatatg gtcagtagtt
    65881 gcacccttat caagaaccgg taaatattta tccattcttg ttcttagctc atcttcattc
    65941 attggtagat attttcctat tgcataacct aatggaagta tttgaaccaa aacagaagac
    66001 ccatcatcac tcttccaatt aaactctgtt ttattagttc cttttctttc agaacatcct
    66061 ctccaaaaca ttgagtattt tatatcaaac ccatttagta tttgtggcat ttgagcactt
    66121 tgaccaaatg aatctggtaa atatcctatt ttcatatatt ctccaaattc atcacaatct
    66181 tttattccat atagtaaatt tctaagtatg gattctccac caactaccat ttcatcagtt
    66241 tgagtatacc aaggacctat tattagcctt ccttcttgaa ctaatttttt tattctctcc
    66301 ttacattcag gcttaactgc taaataatct tctaatatag cagtttgacc atccatcaca
    66361 taatatggat agttatgatt attttctaac atatccatta tttcttccat attatttaca
    66421 agtaatattc tagattcctc agttgagaag taccattctc tatcccaatg catatgagga
    66481 actatgtgta catttttttt cattgcttat cctccaaaac ttatctgata atattttctg
    66541 tctaattaaa aatccaataa aaatactact gagtttacaa aattttttaa tatttatttt
    66601 atcactataa ttaaaccttt gatttaaata tctaataacg tatcttttaa tgtatctttc
    66661 tttgattttt ttgtttccta gttattatta ataatactgc tgatattata gctccaagta
    66721 ctgccgcaaa gaaccatatt gatgctataa taaatcctga catacttccc tctaaaagcg
    66781 ccaatgagaa tatccccgct ccaggcactt gaagacctat attgaagaat gctactatag
    66841 acccagttat ggctgaacca gctaccaatg atggtataac acggattggg tcctctatca
    66901 taaatggtat tgcaccttca gttattccag ccaatcctaa tatccaagtt tgctttccta
    66961 tttccttttc tgattctgaa aataaatctc tgcctaaaac agtagctatt gtaactccaa
    67021 aagctgaaac catcttaact gatgcaaata ttgcataagg tataaaattt ccacttgcta
    67081 ttgctcctat tgcaaatgta taggctgctt tatttattgg tccacctaag tcaaatgaaa
    67141 ccatagctcc tattattgca cctagtagta ttgcatttac tccagtcatc ccatttaacc
    67201 aatttataag accattgtta agtgctgcta caggctttcc tactacaaat aacatcaata
    67261 aacctactac caaagaacca actactggat atacccaaaa acttacgaat ccagctaatg
    67321 tgcctttggc tggtatattc ttctttaaaa attttactat ataaccagca agtaaacctc
    67381 ctaacatacc tccaagaaat ccactaccta ttaaatcagc tgctagacct gctgcaaatc
    67441 ctggtccaag acctggtttt tcagctagtg agtaagacat atatgctgat aatactggaa
    67501 ccatcaatgt acctaacata cctccagaaa gctttcttaa ttgccataac caagaacctt
    67561 cttgattgta tagttcttgt agaccaaatg cttgagatgc taatactgca aatgcaagta
    67621 tcatacctcc tgccacaata agtggtatta tatgagatac tcctgtaagt attgctgatt
    67681 ttacatcttt tttccatgat gatttttcac aatatgatat atcagatgat ttttctatat
    67741 aatcaccttt accttgtttt tgtgctactt ctaaagcttc gtatattatt tcttttgctc
    67801 tttttagtgg agctgctact gacgttttta tgtttggcaa atgttcaaat ctttctgtat
    67861 ttttaggtgc tacatctgtt gcatatatta ttgcatctgc cctttttatt atatctggtg
    67921 tatgagggtc caccatacca ttagctcctt gcttttctac atacacatcc acaccgattt
    67981 ctttaccagc tttaattaat gcttctgctg acatatatgt atgtgctata cctgcagggc
    68041 atgctgttat tgctaatata attttttttg attcattttg aatcctaatc tcttctttta
    68101 tttgttcttt cttttctttt atatctaagt tattaaataa ctctttctta gtttttgaat
    68161 ttaacaacat attcatataa tcttcatttg acagtctagt aactaactca gaaagtattt
    68221 gtatgtgtgt atttccacct tcattttctg gtactgctat taaaaatatt atctctactt
    68281 tattatttgg gtctacgctt tcccaatttt ctattggtgt ctctaaggtc atagctgcaa
    68341 atgcagcttc tttaactgac ttagattttc catgaggaat tgcaagccct ctttcaaaac
    68401 ctgttggaga taaagtttct ctgtacatta cagcttctaa atattcctca taagatgtta
    68461 acttgccttc atcatatagc tttcttgcta agaactttat tgcgtctttc tttgttttta
    68521 ttttttgttt taaaaaaaca aggttttcat ttgtgatttt cttaaattcc aaaattacgc
    68581 cctccttaaa tcatttaatg ctatgaatat agcataaaat aaattatgaa aatgtttttt
    68641 tgaaattata cacacaattt cttcttaaaa aataaagatt tgtgtattta tacacaaatc
    68701 tctatcttaa ccagtattgc tctattattg ctctcataat catcataatt ggaaccttat
    68761 ctgatatatt gtacccattt acttgtttaa ttgttgaata gcaatataaa cttatatctg
    68821 caatttgttg caattgattt tcacatatat gtgttagagc tactacctta caacccttat
    68881 taactgctgt ctttgctact tctataactt ctttcgtttc tccacttaaa ctaagtgcaa
    68941 aaaacatatc ttgttcacct aattttccta tatcatgaag tacttcatgt ctttgactat
    69001 agtaagttat attatcatgc ccacctctaa tttcttttac taatatctca cataaaggaa
    69061 tgttttgacc tattccataa actataatac tcctagattt ttgaattata tctgctactt
    69121 tatttatttt ttcataatct attagctcta atgtcttttt tatatctgat tcaaaaccat
    69181 tttttggctc tttatcctca ttttttaact cttcttttaa attatttttt agttgagaga
    69241 aaccatcata ctccattttt ttacatagcc taactatggt atttgggaca gtaaaaaatt
    69301 tatctgctaa agattgtatt gataatttta ctatttcttc tttgtttttt ctcatatatt
    69361 caattatcat gtcttctgta tctgtaaatt tatactcata tttttttgct ctttcataaa
    69421 attccatagt ttctcctcca tctattttat tattcataag taaaattcaa aaatattagc
    69481 taaaattttc gttaatataa ttataattta atatttttaa tttattaatg catactactc
    69541 tttttattat acataaaaaa tgtgtacttc ctatattgat agagatgatt caagttttta
    69601 ttttgataca tctctatcaa tattttttta gaaaatacac atttttttca aatacttatt
    69661 tttaattaaa actttaggta atctattctt tatatataat tattagactt gaaaatatct
    69721 ttaaaatcaa ccaattcaat attatttctt tttaaccaca ttttaacttc cttatcacat
    69781 agcattgcat gttcatatac tctctcttga ataagagaag atgattttat aagactataa
    69841 tctataaatc ctggatgagt aaccatcaat gttatatctt tatctaaatt tatatatttt
    69901 tcttcaaata atttaacaaa agatttattt aacttgtaaa aatcattctg aggatattta
    69961 cttggcatgc taatacatga atccaaataa tagggacaac ttggtatacc gtattctttt
    70021 gcagcatcac acattgctga tattgataca tcttcttcta taccatgtat atcaatatac
    70081 tcaggcaatt tactcatata caacttgtaa gtttcaattt gagcacaaac ttcttcatat
    70141 acttcttcat aattaaaaac ctctttacct gtcagtagtt gcgttcttct tattttgcta
    70201 gaaacaaatt ttccaccttc atctaacaaa ttctttatct taatatgttc tttgcaacaa
    70261 ggttctccta ttactaaatt aacatgtaaa ccaaaacata catcatcatt ttttaccatt
    70321 tctatagctt gtcttgaaaa aggcatattc accatgattc cagcacttgt aacaataccg
    70381 ttcttataag aagatattat ccctagtgta acaccttctg aatatcctat atcatctgcc
    70441 ctaactacta gtttcttcat tttatgcgtc tgcctgcttt ctcaactcat caagttgcat
    70501 ttccatctca tcctttactt taacaacatc cgttcctatg atgatttgaa ctgatgtatt
    70561 actcaattta attacccctt tggctcctgt ttttttaata ttatcatcat tcaaaatagt
    70621 agcatccttc acttctaaac gtaatctcgt catacaatta ttaagagtca ctatattttc
    70681 ataaccacca caatttttaa ttatctgttt agccatatat gaaaaattac catgagaaag
    70741 ttttaaattc ttttcttttt cactaacttt atcacctatc tctatctcaa cttcacgtcc
    70801 tggtgtttgc acattatctt ttattattat atatttaaat gttacataat ataatacaaa
    70861 gaatacaact cctacaggta taactaacca cgctttatct ccataactaa aacttaagac
    70921 atagtcaatt atacttgctc cattaaatga acccaagcga acacccagta aatatagtac
    70981 aactccacct agaccagaat aaaaagaatg aattatatat aactttggag atgcaaataa
    71041 aaacgaaaat tctattggtt cagttactgt tgccataaaa gaagtcaatg ctccagcggt
    71101 cattgcacct tttacttctt gcttcttttt atcttgtgct gttttatata tagcataaga
    71161 aatagcaggt attgaaaaca tcataattat gtaaaatcct gtccagaaga atcctgcatt
    71221 tgggtctcct gccataaatc taggaatttc tcctcttata acttctcctg ttgatgttgt
    71281 atattcaccc atttcaaata atatgtaagc atttaaaact tgatgtaacc cagttggtat
    71341 tagtgcacgg ttacagaata aaaacaagaa tattcctatt gcaccagctc ctcccaacca
    71401 aaaaccaaat tgattcattg cagcttgtgg atatatccat aataaactaa atatatatga
    71461 taaaattaaa gcaaccactg gtgatagtgt aattacacat ttttctcctg caaagaagct
    71521 taaaaatgct ggaactttaa ctttatatgt tttattgtaa gtccatgcta tacaagctcc
    71581 aacaattatc ccaccaaata cacctgcatt taatgtatca ttcaataact ctaaatttga
    71641 cttaaatatc atatatgcaa ctacggctgc aaccattaca cttgtcttgt ctttagactt
    71701 tgagtaggca ctagctgctc ctaataaaaa taagtagtcc acatttgaaa atactatatt
    71761 agatatatta gatatagctg gcacattgag catatttgga ccaaataata atcctaatat
    71821 acctgctgct ggcattacag ctattggcac tagcatagtc tttcctaaaa gtgacagttg
    71881 ttgcatcatt ttgtttaaga acctcttcat atccaatccc ctttctattt cggtttaatt
    71941 ttttgtgaaa acgatttatt cacattttat attttaaata ttatatttgt ttaaacaaat
    72001 tgtcaatatt ttttattcat tttattttta tcatttagtt taacatataa atccttttaa
    72061 gtgtattgct atatctagtc tattttttta attcattttt tttattttaa aaatatgttt
    72121 aaccatgtat ttgaaacaac ccacaaaata attctatttt gtccttttta tggttgatat
    72181 gattctattt tcactataca tgtctaaaca catttgatat aatttgttca tatcagttca
    72241 ataatattac taaagacctt taaggaggga taggggtata tggctaaaaa taaattttca
    72301 gagatatatg aagtattgaa agaggaaata cttgatggta aatatacaag taatatgatg
    72361 ctgcctactg aacttcaact tattgaaagg ttttcatgta gcagaaacac tgtacgtaga
    72421 gcaatttctc aattaaacac agaaggatat gttcaaagta taaaaggcaa gggtgttgta
    72481 gtgttagaaa atagttgttc taatgatttt tttctaaata tgcacaattt caaaggtgta
    72541 gaatcgattg tagaagataa aaaggttaat actgctacta gtgttctcca cttcagcaaa
    72601 atcttaattg ataataagtt atctaaaaag acaggtttta aagttggtag tgaagtatat
    72661 taccttcaca gactaagata tattgataac atacctaaaa ttctcgacat taactatttt
    72721 ctatgtagta tagttaaaga ccttgatgtt tctatagcac aaggctctat ttataaatat
    72781 atagaagagt gtctaggtac aaaaattgtt tcatccagaa agatatttaa aattgaaaaa
    72841 gctacagagc ttgagttaaa aacgctacct ctaaatgatt ataattgtgt tggtgttatt
    72901 aaaaatagtg tatatactga tgatggaaag ctttttgaat atactgaatc aaagcataca
    72961 cctgaaacat ttgtattcat ggatgttact caaagatatt aaaaaaatta tgtttaatta
    73021 aacataattt ttttacgttt agacacttta tcccatacta acctcactat tctatataca
    73081 attatgtgtt tatacaaata attatttatt gtatatttta gctttaatat tagtttaatg
    73141 cttgacaatt tgtttaaaca tattataatt aacttataaa ttacaggagg tatcaattat
    73201 gaaaaattgg tggaaaaaag caactgtcta tcaaatatat ccaaaaagtt tcaaagatag
    73261 caacaatgat ggtataggtg atataaatgg tattatagaa aaattagatt atctgtatta
    73321 tttaggtgtt gatttattat ggttgactcc tatgtacgtt tctcctcaaa gagataatgg
    73381 atatgatata gaagattatt ataatataga cccaaaatat gggacaatga gtgactttga
    73441 aaaactctta aaagaagctc ataaaagaga cataaaaata atgatggata tggtcctaaa
    73501 ccatacatct actgaacata aatggtttaa agaatctaaa aaaagcaaag acaatccata
    73561 tcgtgattat tatttctgga aagatgctaa acctgatggt tctgttccta ataactggat
    73621 atccagattt agtggaactg cttggaaata tgatgaaacc actaaccaat attatttaca
    73681 tttatttgaa gaaactcaag ctgatttaaa ttgggaaaat gaaaaggttc gtgaagaatg
    73741 ttacaaaata ctagaatttt gggctgataa aggtattgat ggttttcgtt tagatgtagt
    73801 caacctactc tccaaaacac ctggattacc tgatgaccct ataacaggac caaaaggaga
    73861 tggaagaact cactacgctg atggtcctcg tatacacgaa tatttacata atatgaatca
    73921 aaaagttttc aaacctaaaa atatagttac tgtaggtgaa atgtcatcta ctacacctga
    73981 agagtgtatc aactatacta gagaaaatcg agaagaacta tctatggttt ttagcttcca
    74041 tcatatgaaa acagattata aaggaggcgc aaaatggact aatgaaatgt tctctctgga
    74101 taaattaaaa aaagcacaat ctgactttca atataaaatg tatgaaggaa aaggatggaa
    74161 tgctctattt tactctaatc acgaccaacc tagagcttta tctagatttg gagatgatag
    74221 attttttcgt caagaaagtg ctaagatgtt agccataaca ctatttggtc tacaaggtac
    74281 tacatacata tatcaaggtg aagaaatagg tatgacaaat gcctacttta ctaaaataga
    74341 tgaatatgat gatgctgaaa gtaagaatgc atactatcat atgattaaat ctggtattga
    74401 tgaaaaggag gcattgctta tacttcaaca aaaatccaga gataatgccc gtactcctat
    74461 gcaatgggat aatactattt ataaggggtt ttctaatcat aaaccgtggt taaaagttaa
    74521 taatgacaat attagtgtag aaaatgagct taatgattct cgttcagtat tttatactta
    74581 tcaaagatta atcaaatatc gtaaacaata tgatattttt acagaaggta cttatagatt
    74641 gttagatgaa aatcataaaa atctatttgt ttatgaaaga gagtataaaa accaaaatct
    74701 tcttgttata agtaatttca ctcatgataa tgtagtatat aaattacctg aaacttattt
    74761 gaataaatta aactacacaa tacttgaaac caattacact agagatataa ttgaaaataa
    74821 aatggaaata aaaccttacg aaagtataat gatatattat aaataaagtt atttatgtaa
    74881 aaataggtat aacaccctgg tgttatacca attttactaa attcaaagta atgtttatag
    74941 aatatctata gattacaata attatttgac ttctacatca ttatatttct ttgaatatac
    75001 tttagtcata tagaaatata taggaactac aattatcatc ccacctaatc cccaaactat
    75061 cttcataggt tcatcaatac ctgcttttat cagtaataat atacttataa taactgcaaa
    75121 tattggtata attgctcctc caggtattct aaaatttaca tctttaccag catacttctt
    75181 tctcaatatt agtacagcta aacatgtagg aatatattga ataaatctag atacaacact
    75241 tatttgagat aatgttgtga atgaacccga ccatgctatt agtaaagttc ctatgacaga
    75301 tactataatt gctatataag gagctccatt tttattagtt tgtcttatag acttaggcat
    75361 taatccttgt tcaaccaaag cagcagcaga ccttggtgta aatatggatg atgcaatatt
    75421 tatacctcca acagatacta tagttcctat tgtaattata tacccaccta taggacctaa
    75481 catcttaatg gcagcatctg caacaggagt agctgaccct gctaagccat tacccaacac
    75541 ccctatactt attcccagta taagcatata tattactgat actacaaaca ttaccattac
    75601 caatgcttta ggtacatttt tcttagggtt ctccatatct tttgctgcaa cagctaaaga
    75661 ttcaaatcct gtaaaagcat aaaataaagt tatagatact gctacaaaag ctgatgacgc
    75721 actttgacct ttttgtatta ttacaaatgg ggtaaaatta gaacccttta taaagaataa
    75781 tcctacagct ataaataaaa ctattggtaa taattttcct atagtcatga tattatttaa
    75841 gtgcttagat tgtttcattc ccataaaatt tataatccca agccctacaa ctaatatacc
    75901 aattattata tttttaataa gtggttctgc tgcttgtggc catacagatg atagtgttac
    75961 agcaaagaaa tttgccatag tagcccaagc aatcatacta actatccact tcataaatcc
    76021 aacttcaaat ccaacaaaat ctccaaatgc ctcttttgca taaagatagg gccctccatc
    76081 agtatcaaac caactacctg cttcagcaaa acataaagca atagaaacag ctaaaagcat
    76141 acaaaaaact aatactaata tacttgctgg acctatatct gtataagcat caccaggtaa
    76201 cccgaatata cctgacccta ttattgcatt gattcctaat agtacaatac tccaaaaacc
    76261 taacttaatg cctttttcca ttgttattcc ccccaaagcc aacttaatat atttgtattt
    76321 tttatttaga tgcttcttta actattgcca taaatatttt tttcatatta tcataatctc
    76381 tagtcatcat ttctggatgc cattgtatcc ctattacaaa ctcacttcct tccttttcta
    76441 tagcttcaat taaaccgtct ttagatgtag ctgaaacaat atatcccggt gctactttgt
    76501 tgacaactaa gtgatgaaaa ctattaacta aaacttcttt ttctcctagt atttcatata
    76561 attttgtccc ctcctttatc tgtactgtat gtgtaggtac attagataga tgttgctgat
    76621 tgtgtttaat ataagcacct tcaattaaag ataaatcttg atacaaacta cctccttcag
    76681 ttacatttat aatttgttct cctctacata tacctaaaac tggttttttc atatctaaag
    76741 catgttttaa taatttcata tcaaaactat ctctctttgg tgatattgcc cccaacttat
    76801 tatgaggttc ttctttccat atgagtggat ttacatcttg tcccccagat agtataagcg
    76861 catctatatt agatacctgc tcctttataa tctcatcatc atatacaatc ggtacaatat
    76921 atggtatagc tttacacata actactgatt gtatataatc attatttaca taagcccttt
    76981 catagccagg aaacattcct ccttcatcaa ttagtaaatt acctgatatt cctattattg
    77041 gatatttctt tttagccaat ttcttcaccc cttaacttat ttgtgaacaa taaaaaatgc
    77101 tacttctctt aaagagcaag tagcatacca agattttatt tatttgaaca ttataattta
    77161 ttttagaaat actttttttg aatacgagta atataaataa ctttatttac ttatatttta
    77221 ttttaatcct gtttagtttg acttttttaa attgttttaa gtacaactaa taggtaaaat
    77281 aggcatttag attttccttt ttatcaacct attaaatact gtttaatata ttttttcccc
    77341 ttctatttaa ttcacttccc tttctaccag attcacatat tatcaaatcc agctctttta
    77401 acatattaag tattcctctt acctcacctt ctgttatatt gtagcctaat tcatttattg
    77461 aattaacaat accatttcta cctatatttt tattaagatt attggcattt tttaaacact
    77521 ccattactaa ctttactttc tctatagatg ttctatcctt taatatgctt atttctttac
    77581 taaaatcttc agttatattt aataagttat gaggtaaatc atttattaaa ataacatcct
    77641 cagaactcat aatgttaata tacatagcag tattttttaa ttctcttata ttacctggcc
    77701 attcatatgc attaataatg tcttgaacat cttgactaat tgctctcttc cttttcataa
    77761 aatgctttaa cataacttct atatcttctt ttcgctctct taaaggtggt atatttatgg
    77821 gcaatacatt tatcctatag tataaatctg ctctaaatct atctcctttt atcatttcta
    77881 ataaattttt atttgtagcc gcaattactc taacatctat atctattaca ttttctcctc
    77941 caaccctcat tacttgattc tcttgtaata ctctaagtaa tttgctttgt aagtatatag
    78001 gcatgtctcc tatttcatcc aaaaatatag ttccattgtt agcctgttca aataatcctt
    78061 ttttacctcc ttttaaagct cctgtaaaag caccttcttc atatccaaaa agttcacttt
    78121 caagtaaatt ttctggcata gctgcacagt ttattgctat aaatggctga ctatgtcttg
    78181 gtgatgcatt atgaatagac tgagccatga gttcttttcc tgttccactt tctcccgtta
    78241 taagaattgt ataatcagat tcagctattt tcatacctaa tttcttagtt ctttccatct
    78301 tcgttgaaac tgtttttata tcatcaaaag tatatctagc aacttgtccc ttttctctaa
    78361 gcttctttga taggttttgc tccaattttc ttatgtaagt tatttcttga atacaaaaat
    78421 aatatccagt aacccttcca aaactctcta tattatactt atttacgttt aagtacttat
    78481 tattaaattt tataacttca tcatgtgcct tactcaaact aaaaatcttt ttaatattct
    78541 cattctctaa ttcatctata tttttcccta taatattact ttgaatatct agtatatccc
    78601 ttaaactatt attacaaata attatgtttc ccttatctga taccattgcc atacctaagt
    78661 ttgataaatt tattatggaa tttagttcat ctgcttttat agtaagttct ttgtatttag
    78721 tatttattcc tgaatataaa ctaattattt catctgtata cttaattagc ctctcagtaa
    78781 tttctttatc atccagtttg agttttgcta ttatttgaat aaacgttgaa ctatcaatat
    78841 acctatggcc taaatcaata acttcttcta tatattttgg aactaaactt gactctcctg
    78901 gcgttatagc tatcttaata tttctatatt cttcattggg attgtatggt ataaaattca
    78961 aatgatttat tcctatattg tataaattag atattgtttc caatacagta tgcttattat
    79021 cgtttactac taatacatct attccttctg gcaatgaaaa taacttatat atatcctttt
    79081 gctttatgct cctatttata gttattatct ttgagacatc ttccacatat ttttttattt
    79141 ttaatactct atcatctatc attaccaata ttatgtcatc ttttattaat tccttctctg
    79201 ttattttatt taaataataa ttttttatca ttactttatc tttaaagatc atttctagat
    79261 ttttatttaa gaaacattct aatttagaca acccatcagt tactactgct attgttttca
    79321 tttgattact ccttttaaaa tatactctac tcttttataa tatacatttg attttcactt
    79381 taataacaca aaaaattgta catttagtat actaacatat tcctttatta gttttatgtc
    79441 cataaatatc atagttgcaa tatctatcat atttcttaaa cttctataaa gcaaaaattg
    79501 tgccaaatta ataattttct taattttatt ctaaagttcc tcaatagtat ttacagactt
    79561 agcacttata gcttataagt ctttatatta ttaatctatt tttataggtt aattgtacaa
    79621 atcatatatc acttttacct attaaatgca ttataaaaaa ttgtatttat aaacactatt
    79681 tactactctt ctaagtacaa acagtatcta catactataa cctttcacca ttagattcta
    79741 ttacttgttt ataccagtca aaactctttt tcttaatcct agacaagtct ccacttccat
    79801 catcatactt atccacgtat ataaacccat atctcttggc catctcacca gtagatgcac
    79861 ttactaaatc tatacatccc catggagtat atccaaataa atcaacgcca tcttctacag
    79921 cttccttcat ttgctctata tgtgctttta aatactctat acgatagtca tcatttatca
    79981 ctccattaga ttctttttta tcataagctc ctaaaccatt ttcaactacc atcataggta
    80041 ttctatatct atcataaatt tcatttagtg catatctaag cccttcaggg tctatttgcc
    80101 atccccattc actagactta agatatggat ttttcactcc acctaatatg ttgccacttg
    80161 tattatcttc tttagaatca ctacttacac atgtagacat ataataacta aatgtataaa
    80221 aatctacaca accttctttt aaaatttcta aatctccttt ttccattttt atttcaatat
    80281 tattttctcc aaaatatcta ttcatataag ttggatattc gccacgcact tgtacatctc
    80341 cacagaaata attcattact ctactatctc tttgagtttt aactgcatca tctggattac
    80401 aagtataagg atacattgtt gcaaatattt gcatacatcc aacttgataa tttgaatcta
    80461 tttcatgagc taacttaaca gccttagcac ttgctacaaa ttgatgatgt aacccttgaa
    80521 aacgaagttg aggtatatct acttggttta taaaatctgt agtcccttca tttaatattc
    80581 cttgtgataa gtagcctccc ataggcattg tagctgaatt tatttcatta aatgtcagcc
    80641 aatattttac ttttccttta tatcttttaa atattgtttc acagtaattc atatacaagt
    80701 ctattacatc tcttgaaacc catccattgt atttctctgt tattgcaaat ggtatttcat
    80761 agtgagatat tgttattaat ggttctatat tatattttaa acattcatct atcacatttt
    80821 cataaaattt aagtcctgct tcatttggtt ctttctccat tccagttgga aatattctag
    80881 tccatgctat tgaaaatcta aataccttaa atcccatttc tgcaaataaa gctatatctt
    80941 ctttgtatct gtgataaaaa tctattgctt catgacttgg ataaaatgtt cctttttcta
    81001 taactctcgt tattctttta cttacttttt gagaacctcc agtacacata tctgaacaag
    81061 aaggaccttt cccatctaaa ttccatgctc cttcacattg atttgctgct gttgctccac
    81121 cccataaaaa atcttttcta agtgccataa ttctacctcc aataatttat ttatagtaac
    81181 tttattttaa aataaaaata catatatata attcttaatt aatagaactc tcttttacgc
    81241 taaaacagtt atcaatttct gactattatt tacatgcttt tgtgtatttt ctacaatatc
    81301 cacataatca gaataattag taacaactac tggtgtttct aagcaatatc cttcttttga
    81361 aatactttct atgtcaaaag ttattaatgt ctgacctttt ttaatcttat ccccttgatt
    81421 aacaaaagat ttgaaatatt taccttccaa ctgaatagta tttaacccta tatgtatcaa
    81481 tagttcacaa ccattatcag atataattcc tattgcatgt tttgttggaa ataaagtcat
    81541 tacagtccca tcaaatggtg ccactacttt tccttcttta ggtgttattg ctacaccttt
    81601 tcccaatata cctgttgaaa acgctgcatc ttgtatctgc tccaaagcct ttacttcacc
    81661 ttttattggg ctatatacta cttctctttt gtttttttct ataacgcctt gcccattatc
    81721 atctttagaa atttctacta tagcatcatc tttccaaaaa aataatgtta aaacaaatcc
    81781 tactacaata gctactatta ttgctattat agttgcataa acacctttca tatctccagt
    81841 tgttggattt atataattag gtataccaaa tattccaagt ccacccatta cataactttt
    81901 tacattcatt atccccatta cagctcctgc cacagcacca cctacacaac tatatatgaa
    81961 tggtgttttt cttggtaaag ttattccata tattgcaggc tctgttacac cacatatccc
    82021 tgaaataact gcaggtatac acagctcttt tatcttctta tcttttaatt taaagtacat
    82081 tgctgctact actgctgttt gtgcaaaaga tggtacaaat actcccacta atattgtgtc
    82141 aaaccccata gtcataaggt ttgacatagc tattggtact aatccccaat gtagaccaaa
    82201 tattacaagc acttgccaca ataaaccagt taatgcacca cataaaattg gtgaaaagtt
    82261 aaataaagct aaaaatccat ttccaactat attagaagca taggtagcta taggccctat
    82321 aaccataaat cccaatggta aagaaactaa taaaactgcc attggtacaa agaaattctt
    82381 tactacatct ggtattattt tagcaaacac tttctccaac ttagctgcta ataatactat
    82441 taaaattact ggtactactg ttgaagtgta gttcattgct attactggta atcctaagaa
    82501 agttatatat actggtgatt caaatattgt tccagtaaat agattgtata aaggttgacc
    82561 tgtagatgct agagtatcta attgaatacc tgaatagcat aaaactgcac ctataagcat
    82621 tccaataaat ggttttaatt tgaatttctt agcagctgta tatccaagaa ttattggcat
    82681 atactggaac attgcatctc caattgcatt taacataata tatgcaccat cagtttctcc
    82741 atacaaaccc aatgctgtaa atactgcatt aaatcctttt atcataccag cagcacacag
    82801 aactcctaat attggttgaa aaactcctga tattatgtct ataaacttat taagtaatcc
    82861 ttttggcgca tctgagtctg aattatctat tgtatctcct cctaatccag ccaaatcgca
    82921 cacaacttca taaacatctg gaacatgatt accaataact acttggtatt gacctgcact
    82981 tttcattaca gtaactatac catccatttt ttttagcaca tcatcatttg ctattgactc
    83041 atcttttaat ttaaaacgta gcctcgttat acagtgagat aaactgctaa tattatcctt
    83101 tcctcctaca ttcgcaatga tttcttttga tagttgttca tactttctca ttttttctcc
    83161 tccattttct ttttgaaata tgaaggtttg gccaacttaa tgtcaccacc caatatatat
    83221 aaacctaatt attaggattt atatccgact taatactttt acttaccact ctttcaacgt
    83281 gaatagttaa gtataatttt tcctcatctg ttaaatcgta gttgtatttt ttatgtataa
    83341 attcatccaa cttttctatg aatttataag catttttata tttaactttt attacattta
    83401 ataaatcatc ttcattattt tcatgtaaat tattactaac aattcgttct gcaaaatact
    83461 ttaagtgagt aataaatcta tgataataaa ctgaatcttc atcaaatact attctgtaat
    83521 ggtatttaac catatttgaa atttcttgta ttatttgtgt aatctcatat atagttttta
    83581 tactttgatc catttgtgca ttaactatat gcagtgctat aaatccagct tcatcttctg
    83641 atagtctaat ttcaaatttt ttttctatat aatccaatgc ctctaaccct attttaaatt
    83701 cactcttata aaatcttttt atatcccaaa gtaatacatt ttttacactt actccttcac
    83761 gagctctttc tattgcagta tacatatggt ctgttaaaga aatataaata ctatcattta
    83821 atcttttccc tagtttttct ttagcatata aaattatttc atctgataat tccatatatc
    83881 ttataggaat atctgctatt agttctttaa atttatcaga tatatttggg tctgaaagtt
    83941 tgaatatctt atcaatttta tcctttgata tatgctctcc tgtacgtttt ttataagcta
    84001 aacctctccc cataactact atttccttat gattttcatc agttgtaata attacattat
    84061 tattgagtat tttttcaata atcattgtga tgcccccttt attagcatta agttaaaata
    84121 aatctttttt aattatttag aatatttaaa atgtaattta ttacataaaa aaaaacctat
    84181 ttattacaca ttaaatagac tcccatgagt cctgagtaat aaataggttt agcttgtatt
    84241 taaacaatca ctatccgttt gtattttatt ttaattatta tttattatat gttagcaaat
    84301 ttatttttta gtgtcaactt tttctcaata ttttaatttt aaataaatta tataaattga
    84361 atcttcaaag tactattttt atcaataaac aaaataaaaa aggtattact ttctttaagt
    84421 aatacccatt tatttctaat ttaagtggtt tttcttaatc tcatcaagta gtccatcttc
    84481 taaataaatt tctaatgcac atcctaccat tgcctttact gctttgtgaa gtttatcata
    84541 agcttcctca gaatttgcat aatttagaaa ttctttttca tgaacatata ccattggact
    84601 tatatctaag gctatttctg tatacatagt agggcaaact tgacttacat tacctatatc
    84661 tgtagaacct actggaccat ctttaccttc taatatatct gttatacctg cctcaatcaa
    84721 attattcttt gttatctttt gaagtacttt gttatttatt atgttgtcaa aagaattttc
    84781 aaagtatcta taagaaagct tagctcctgt cattaactct gcaccttttg cacagtttat
    84841 aactttttta gttacttcat ctaaataact tctttcagca gctcttacga aaaacttaca
    84901 agctgctttt tctggaacta tatttggagc ctctcctcca tttgaaacta taccatgtat
    84961 tctaacatct gattttacat gttgtcttag tgcatttatc ccagcaaaag taagttgtac
    85021 tgcatctagt gcattgactc ccatatgagg gtgactagct gcatgtgctg ataccccttc
    85081 aaaactaaac tctaatgaat ctatcgccaa agccttacag tttatattat taaaagcttc
    85141 tatatgcatt tgataactta catcaacatc atcaaaagct cctgccttaa ctaaatcaca
    85201 ttttcctcct gttgtttctt cagctggtgt tcctattagt acaatttttc ctttaaaatt
    85261 ttcttttaac ttacttaata ctatacaagc tcctagtgta gatgctgcaa tccagttatg
    85321 tccacaagca tgagctggtt ctttattttc tccatatcca ggaagtgcat catactcagc
    85381 taaaaatgct atggtaggtc catcattatc tcctaattca gctctaaaag cagtctccat
    85441 attacaatat ggataaactg tatcaaatcc atattccttc attttttcta ctaagtattt
    85501 tgatgatatg tattcttctt cccctaattc aggattgtta aatataaaat tacaaacttc
    85561 ttcaatttct tttccaagtt cattctctat ctcataagct ctcttcttta tactctctat
    85621 cataatttac ctcctactag aatcctagat ataatcctat tgtcaaaaat gccattacaa
    85681 gaattgttaa tactaccatt aaaggaatta cgaatctaac ccactttgac caaggaatat
    85741 tggctactgc tagtgctccc attaaaacac ctgatgtagg tgttatcaag ttagttacac
    85801 ctataccaaa ttgataagct gtaactatca tttgtcttga tacatctact aagtcgccaa
    85861 gtggtgcaag tacaggtatt gtaagtgatg caagccctga tgctgatggt attaaaaatg
    85921 ctattaatcc ttctattaag aaagttaaat ttataaatat agtttttggt aatccattta
    85981 ataaatttgc tgcagaattt aaaattgtat ctattataaa tccattttct gctattatta
    86041 caattccacg tgcaaatgct atacacaatc ctgctgtcat taaatctcct gctccactta
    86101 taaatgaatt aactatatca tcttgtttaa gacctgatgc tatacctgat attataccta
    86161 tagctgtaaa tatcattgca atttcagtta tataccatcc caactttaaa actccatata
    86221 tcataatcgc catacctatt gtgaaaattg ctattaccat ggcttctctt tttgtaaatt
    86281 cctttatatt tgtatcacta ttaaccattg attcttgatt tagtgatata tcgtgtacta
    86341 aagatttttt agggtctttt ttaactttat tggcatacat cataacaaat cctatagata
    86401 tagccatata tattataaac ataacaactc taaactctat tccagaacct ggtgctattt
    86461 gtgataatgc ttgtgctatt cctacagaga aaggattcgt agtagctgct ccaaatccag
    86521 ctgttgctcc tataaatact gtggccactg ctgtcaatga atcatatccc atactggtca
    86581 taagtggaat aaatatcata taaaaaggta gtgtctcttc actcattcca aacgtcgtcc
    86641 cacctagtcc aaatagaatc attgaaatcg gtattattaa aaattgaaac ttcttcattt
    86701 tatttacagt atgagctata acagcttcta tagctcctgt tttattaact atcccaaaag
    86761 ctcctccaac tattaaaaca aatccaacta cttcagcagc atctataaac ccgtttatag
    86821 gtgctgtaaa tacatcagta attccttgtg gatttgatgg tgctttttca taagttccag
    86881 ctacaacaac tgaccttccg tctatatcta ctctttcaaa atttccactt ggcactatcc
    86941 aagtaagaat tgccattaac actattagca aaaatattat tgtaagtgtc tgtggcatag
    87001 taaacttttt cttttttaaa ctgctcattt ttaatctccc cactttttaa attaaatatt
    87061 ttattattgc atccctttat cctattttgt atagatgtaa aaaagctcaa catggataca
    87121 tgttgagcta aatgtgctaa atacaacata cctgcatctt ccctttatct tgtttaagat
    87181 agctctccat caaaattcgg gataattttg atgacagtac tgtacctatt aaatacagtc
    87241 ccagcactag cttcggacac tagcacttcg gcacttactc cttttatatt gcctcattgg
    87301 tttccaacct cttcaatatt aatcttagta aatgcaacct ctacctcaat ctgtttgtta
    87361 gatgaggtaa ttcttattaa tttttattaa ttcaaagtaa ttgtagcata atatgtcgag
    87421 attgtaaata ttttcagtat aataattctt actttatgat ttcattccat ttttcacaaa
    87481 taattttttc tccatataat ttaaatactg tagtatattt taaatacaca caaactttcc
    87541 acaaatttta aattattata atacctttat aataaaaaat aaaagctatg taaacttatc
    87601 aatttacaca gctttatata cattaatcta ctccaagtac agctttaagc tttgatgcta
    87661 ttgtaataac tcgtggacca aatataactt gaactgagtt tttcttttta acaactccac
    87721 ttgcacccaa ttcatttacc cagaagccat cttcatagaa tccatcaatt gaatttagct
    87781 ctaccctaag tctagttgca cagtttgtaa cattttttat attattactt cctcctaaac
    87841 cttctattat agaatgtaca agctttgtat ctttatcatc tacttcctct tctactgcat
    87901 tgttgtatgc tgttttacca gcatctacag attgtctttc tctatattct ttcttactat
    87961 aaagcttaac ttcttctgaa cttcttcctg gtgtttttat atcaaacttc ttgattgccc
    88021 atgtaaatat tatataagta actataaaaa atattggtat caaaaataat aatggtaacg
    88081 catccacttt ttgtggttgt aataaattag gaaacatatt tctaaatgat tctcccatta
    88141 tagaaacatt aaacatttct gtaaatacat acgtcaatcc tgccagtggt acatatgcta
    88201 caaaatacaa taatggtgca acaaatagga atgtatactc tattggttcc gttaccccta
    88261 ccaacatggc tgttactatt gctggtatca atatacttat tgcttttttc ttattagcat
    88321 tatcagccaa cttatacata gctaaagctg ctgctggtaa tattgcaaaa tgtattaata
    88381 aacgacctgt tgtgaagtta cgagttatat aagatagtgt atctgggtct cccatttgag
    88441 ctaatcttat attattaaca ccctcgtaaa ttgctccacc tacttccatt gttccaccta
    88501 ctcttgtata ttctataggg aatgcaatta agtgatgtat accaaatggt aataatgctt
    88561 tatccattgc tccaaaagta aatgttccta ataatccact agaacctatc caattaccaa
    88621 gtttagataa tgccaaccct actggtggcc atataaagta cattgctgta cccaatacta
    88681 ttgatgctat aaacatcata atcattgaaa atcttggacc tgagaaaaaa gataatgcac
    88741 taggaagttc tatgtcaaca aatttgttat gtactgcact agctacaaat ccagctatta
    88801 tagctccaaa tatactacaa tcatatgaaa agattcctaa gaaagaacca tataaagaac
    88861 ttcgactata tgcttctgcc tctcctaatc cagatgccat aaacgcttct gctgtaactg
    88921 tatctgaatt tataccattt gctgctaaca atgtacttat aactgtgtgc attcctataa
    88981 ataaaacaat tcctcctaat gcaccccatc ctttttcctt tttagataat ccaaaggcta
    89041 tacctgtaac aaagaatatt ggtaagtatc tcattaccat aaatcctaaa tcgtttatta
    89101 ctttaaagaa tacatttata aatgaccctt cttttattag tccgctaagc cctaatgcag
    89161 aaacgttttc catatttgca aaagctgacc ctagtcctat aaatactcct gctacaacaa
    89221 gtaatataac aggtatcatc attgctccag aaagtacttg taatttatcc ttcatagtat
    89281 tatttcctcc atttttattg aaatgccagg ctatataatg catatatata tacttctatt
    89341 gctttgtata atgtatctat acttatattt tcatttgcct gatgtgaatt tggattatcc
    89401 ccctctattg tgggtccaaa tgcaactcca ttttttaaca ctcttgcata agatgcagct
    89461 ccttttgtaa aaagttcggc ttcttttcca aaaccttgtt tatatgcatt tgataattta
    89521 tttattaatt cactatctgg ttttacatac agtaatttta aatccttata tatatcaact
    89581 attaaatttt ctttttttcc aatttcatta attctattag ttataaactc ccaacttata
    89641 cccttaggat atctaaagtc tattttcata ttcaattcat ttttctctaa gaagatactc
    89701 attatacata ttgtagaaac tcccatatct acatcttctt tatatatccc taattttttt
    89761 ccatgattat catctgtaaa gtatgaattt aaaatatccc ttattataac gcctttatca
    89821 tttagttttt taattttctc taaatctttt gctaaactaa atgcacagtt atatgacctg
    89881 tgtggatttc tcgataaagc cctttctcct gtatatctta ctaatacttt accttcttct
    89941 agttcttcta tctctgtata ataagctaat gattctacta aatctttctt atcattagtt
    90001 ttaaaaacag cctcaatttt gtcacataca aatccatctt ctttgtttga ctttatatca
    90061 actaaattgt gatttctaaa tttatcttta tctattttct ttcttagatt gaagtataaa
    90121 ataccttttt ctccatttac tattgggaag tttccatctg gagaaaatgc aaattttggc
    90181 tgttcattga cactaaagta atgctccata cattcccaag tcgtctcttc agctccaccc
    90241 actatcaatc taatctttct cttcggcttt tcattaagtt ctcttaagaa aagtaatgca
    90301 tataaaatcc ctatcaaagg tcctttgttg tcattaactc ctctaccata aagatagtta
    90361 tctttttgac atactttaaa aggtttactc ttccaaagtt ctttattata tattggcacc
    90421 acatctatat gattaagcac ccctactact tcttctcctt ctccatattc tatatgaatt
    90481 gcatatccat caaagtcttt tacaacaaaa tctttgtctt ttgctatttg aataagctta
    90541 tcgaaggcat ttcttatttc tatcccaaat ggttgattta tatctgttgt actttcatct
    90601 ctaattgaag gtatttcaac taaacttcta atatcattta tgatattttc ttgattggat
    90661 tttaaataat caataattag ttctttgttt actatcatct taattattta tcaccccttt
    90721 atattaattt tacaaagcat ttcagttcat atctacatta taaattttga atattatttt
    90781 ttcaatattt caaatacttc tctaagttac cataatgata cggtaaacaa tcaacagatt
    90841 atgacaaaat catactatac ctattactaa tattttaact tatctattac gtctttttta
    90901 atgtaagttt attccaaaaa tacataaaaa aagatgtagc cttagctaca ccttcttata
    90961 tctattcaac ttccattatt atgctattaa tatctacatt tccaggttct atcctatctt
    91021 ttactgttct cttaacagaa tcagcatcat ccattataac tacaggagtt attatattta
    91081 aatcatttga cgttataaaa tctaaatcta cttccattat taaatcttta gcttttacct
    91141 tatctcctgc ctttacctta acatcaaacg gctttccttc tagtgacatt gtctctaaac
    91201 ctacatgtat taatatattt gtattatcat tactcttaat cattattgca tgctttgtat
    91261 caaatacatt gactatttca ccatcaacag gagaatataa cttaccttca cttggtatta
    91321 ttgctactcc atctcccatc atcttttggg caaaaactgc atctggaaca tcttctaagt
    91381 tcactaaatt tcccttaaaa gggctatata ttttctcaat cttattttct tttttcttaa
    91441 atatattaaa cataacacac tctccacatt ataaaatttt atgataatat ttttcttaaa
    91501 gcttttgcta caccatcatt tacattagta tcagttacaa aatctgccat agattttact
    91561 tcatctgtag catttcccat agcaatacca agacctgcaa atttaatcat ggatatatca
    91621 ttttcattat cacctataca aataacttct ttgctatcta tacctaatat atcacaaaat
    91681 tttttaactg caataccttt tgaagtacct ttactcataa cttcaaagtt tatctttcca
    91741 gaacttacta cttctaaatc ttcttgtttt ttaaattctt cctttgcttc tctcaaagaa
    91801 tctacgtttt ctgagaaaag tatacatttt aaaatttcat tatcagcttt ttctaataaa
    91861 gtttcacaac ttgcattttc tattatttca attctgtctt cttctttatt tttactattt
    91921 attaatctat atggatgttc ttcacctatt tcattgttgg atataactat tttacagcct
    91981 ttaaaatgtt tgcttaaatt atacttttca cctattgtat agattttctt tagagcttct
    92041 ttgctcaaaa ccttcttata ctcatatccc agtaatttaa aatatgtccc attagttgat
    92101 ataatatata tatcatcccc taaaacactt gtaaaatatt tcatagcatt ataaggtctt
    92161 cccgtaacta aggctacctt gatacccttt tccatgactt cttttagtgc tttaatattt
    92221 tcttctgata cttcaaatcc tttacccatc aaagtaccat ccatatcagt acaaattaat
    92281 ttatatcgca tttgtatcac atcctagtaa gatttttcta atatttcaac aaggtcttct
    92341 ttctttccaa aaggtaaaaa tcctaaaact ggacagcttg ttatttgttc taattgttcc
    92401 tttgttacat taaactcaga catttttgtc ttaagtccca ctctctttaa aaaactttct
    92461 atttcatttc ctaaagtttt agctgcttca tcatcttcca ctttttctaa ttcttggttt
    92521 agcattctcg ctactttggc aaatttctct atattatcct tatagaactt ttctgcaaat
    92581 tttgggaaca ctatagccaa agcctcacca tgaggaatat gtgtaactcc tcctattatt
    92641 tcacttagtg gatgtggtgc tgctgctcca gcatttgcca gtgaacttcc tcctaatgta
    92701 tctgctactg ctaactttgt tctgtattct atattatttt tatcttccat tactttaggt
    92761 agatagttaa tgactaattc catagatttt tcagacatta tctctgtaaa atcattagca
    92821 tttggattta tataactttc aaatgcatga gtaaaagcat caaatgctgt tgatgcagta
    92881 attctctctg gcagagttaa cataagctct gggtcaacta tacattcttt tgagaaacaa
    92941 ttttgatgaa atattgtatt tttctcgctt cctctcgata ttactgctgc ttgtgtaact
    93001 tggcttcctg ttccagaagt agttgataca gctattaatg gtaattcttt ttcacttacc
    93061 ttatcgtata ttttaaatgg atttgtatat gtactaaaca tataatccca atctatcttt
    93121 tctaaaccaa aagtcaatgc taatactttt gcagtatcta tacttgaacc tcctccaaca
    93181 gctaaaacaa catctacaga ttctttttta gctaattcaa acccttcatt tattaattct
    93241 acagttgggt ttggctctac tctatcaaag tgtacaactt ctataccttt ttctattaaa
    93301 ttcaatgtaa ctcttttata taacttgtct aatggctcta catttggagt agtaactaac
    93361 aaacatttct ttccatattt tcctattatt ctaggtaatt cacttaaagt tccttgtcca
    93421 aaatttattc ttgatggttg ataaaagtta atcatatttt tcctccctta aattattgcc
    93481 ttcatattca aattatacat atcataggat ttttaaggca ataccttcac tgttaactct
    93541 tatttactaa agcttatggc aattattaga caatcaatgt tttattatta ccatatcatt
    93601 atgtaaattt tattaagtac ccatgttata attttataat caatcttatt gtataattga
    93661 cttaataata aaaatagagt atatacactt ttttatgaca aatagcttaa aggagaatta
    93721 aattatgaaa tatttgatag acagtgctaa tcttgatgaa attcaaaagt gtttaaagta
    93781 tggatttaag ggaataacag ctaatccatc tatgtatctt aaagagaaag taaacttcca
    93841 tgaatttatt gagacatgtg ctaatttaaa tccagaaata ttaactgctg aagtcattgg
    93901 aaataattta tacgaattaa ttaattcatg tgataagata ttaaatataa ataaaaacac
    93961 tataataaaa ataaatttct ctgaagttgg cttagaactt ataaacatat taaatagtaa
    94021 aggtgtaaaa actgcatgta ccttgatttt caatgtaaat caagcatcat tagctataaa
    94081 tgcaggtgct gattacctat tcccttttat aggtagaaat gatgaaaatg ggaatgatgg
    94141 tataaaaaac cttacagaaa tatgtaacct tatatcaata aatggatata atactaaagt
    94201 tgttgcagct tctattaaaa atgtattcca cttaactcaa gcatctcttt gtggatgtga
    94261 ttatgcagct gtaacatatg atttatttaa aaaagcttct tttaaccagc ttacaataga
    94321 tggtgccaat acttttgaaa gagattggtc caaggttaat aaatagtaac taagatttag
    94381 gagggtttgc attgaacaaa agattattag atattgctga attattactc aacagctcag
    94441 actatattac tgtagatact attgccgaag aattaaaagt tagtaataaa actattcgta
    94501 atgaccttgt tattttagat gaatggcttt tagagtttaa tttatcccta gataaaaaaa
    94561 ctggttcagg ggttattatt ctaggtaatg aagatataaa acttaaagtt attagagata
    94621 tcaatgataa atcaaattgt atatatgcat actctcctga agatagaaaa aggtatattc
    94681 taagtaaatt atttattaaa aattctaagt ttaggattcg tgatatttgc aatgaactac
    94741 atgttagtag agctactgtg cataaagact tggtagttat tcaaaatttt ttgcaaaact
    94801 ttaaagtgac tctagttaga aaaaccaata atggagtata tttagaggga aaagagaaag
    94861 atattcgaaa agcaattttt gaacttgcta cggttaataa aagttatact gaacttaaag
    94921 aaatattatt ctcgaaatct aatgaatgca aagatacccc ttcaacaaaa atatttaagg
    94981 agctatttaa ctatgatttt gagaaattat ctcaaatcac tctacacacc ttacaagtag
    95041 aaaaaagtaa ccttagtgat gagtattata taaacttctt aatacatata gctatatgta
    95101 ttaaaagaat ttcaattaaa agatatatta acttatctga aggttttttt agagaattat
    95161 tagaacatga atactttgaa aaatcaaaaa ttttatgtgc taacctaagt gaagcttttg
    95221 acgtagaatt tattgatgaa gaggtctgtt atatccttct tcatatagaa ggattactta
    95281 aatcaataaa gaaaactact actcttgaac ctcttaaaaa tgaagaaatt acagagtata
    95341 atctatctga agcaatagct gttcattggg gaagtatact aaatttaaac ttaaaggatg
    95401 atttaatatt aataaagtct cttgctaatc accttaaatc agttctacac agaataaact
    95461 atgggtttac tattacaaat cccatattaa atgatataaa gagaacattt ccttatactt
    95521 ataaggctgc taaagagtct aatactgtta tcaaacagtt gatgaattat gaagtcaatg
    95581 atgacgaaat aggatatctt gctttatatt taatatcagc aatagataga agcaaatctc
    95641 cccttaatac tattttaata tgtcactgta gtgatggtgt atctaattta ttagtccaaa
    95701 agttaagttt tgaatttaat cagattaata tagttaaatc tatacctcta tcttctattt
    95761 catttacgaa ctttgatgat gtggacttaa tactaaccac tgctcctgta gactttgaac
    95821 ataatgctga actgataaat ataaatgctc ttttaagtaa gaatgatatt tctagactta
    95881 gtacaattat aaaaaaacta tattctgaaa aaaataaaat attatcttat aaataaacaa
    95941 aaataggctt gtctcaacat agagataaac ctatttttca ttgtctaaat gataaaaata
    96001 tatttgattt catagaaaaa gtgcctcatg aactaaaaaa ttcattttga gacacctcca
    96061 ttttttatat ttaagctttt tatttaagct tttatatttt ttaaactttt atatttaaag
    96121 taaattttat tatttttatt gagcacaagc attatttata ttattaattt cttcagtatt
    96181 cttaccaaac tttgtagtta caaatcctat tgtttttatt atgagtaata aagatatcaa
    96241 tgaatatata gatatcataa aattcttact tattattcca gacgctacta ttataaatcc
    96301 atctagcata aataatataa ctggcaactt cagaaactta attaccttat taataagcat
    96361 tgcaactaaa tcagttccac cagtactaca gccccttatt attgctatcc ctatagcagt
    96421 tcctaccaat aacccaccta tcactcctgc tactaataga ttgtcagtta tgtgagtttg
    96481 tggtattact tttaagaata atgggcccat aagagttata tataatgttt ttattccaaa
    96541 acctttccct aaaaataata atcccattac taaaagtggc atagatattc ctaatgatat
    96601 attctcaaca ggcattgata agaatgtgct aagtacgact gataatcctg ttatccctcc
    96661 tggagctaat gtatgttctg caaagaaaaa attaactgct aatgccatta aaaatgctga
    96721 tattgttatc agtaaataat cttttatcat ttcttttttc atttaataac ctcctatgat
    96781 tatattattt cagtataaaa aatcaattaa attagtctag cttcctgtag tttttcataa
    96841 aattaatgct tccttaaaac attaagaaag catctctttt atcatagttt attatcttat
    96901 tctaaaccaa actttgtagc tgctttatct atagcttttc tacaattctt aacacctgct
    96961 gttataccat ctttatcctt gaatattgct gaagataata ttacaacgtc tggattacat
    97021 tctattaatg gctccatatt gtctgctctt agtccaccat ctattgctaa ttcacactta
    97081 ggattctttt cgtctatcat ttttctagct ctttttacta aatcaacagc actctttctc
    97141 catccccagt tatcttttcc atcagtttca tcaacaccat gaacgactat ttgcaatcta
    97201 tctatatcat atatagcttc atcaacaaaa cataatggtg tgtaacatcc aattgttaat
    97261 cctactttca taccaaattc tctacagtaa tttattatgt atgcaagagg agcacctaaa
    97321 aaatgttctg ctggtattat caacaagtta catcctgctg ctgctatttt ttctataaat
    97381 aatctatcac aatctcttgt gtatatatgg cattctattg gtttatctgt cactggtctt
    97441 attgcttcta ttatttgatg accacccata agttgcatat tttttaagtc atgcatatct
    97501 gctgcatctg aatgcacgta gtctacacct gcatccattg cttctttaac tacggctgat
    97561 atatgaccgt aatttacatg tgctaatcct gctgctattt ttatgtgttt catatttgta
    97621 tacctccata ttttacttta attagtatta aatattagtt tttgatattt ttttaatatt
    97681 ttttaatcca gtattacttt tatttagcat tacttttatt gctgtatata tttcttttat
    97741 gaacttattc tgcttttgtt aactgtgaac ttattttgct ttgtaaatta atattacact
    97801 gatatatttt ttttcacaac actttgacta ctccttaaaa gtacctttat attgtggtaa
    97861 attatagaca ttaattgtat ataaaaagtt agttttttac cacattctta tggcatttat
    97921 tgtctatact aactcaattg atatagctag ttcatttaat atataatcag actataaata
    97981 taaatttact cgataaaaag taccattcat aacaatatta atgaaaggga gatgtaaaat
    98041 gagaaactgt attattgccc aatctggagg ccctacagct gttatcaatg cttctgcagt
    98101 tggtatttat gaaaaaaatt tagaaactaa atactttgat aaagtatatt ttggtatcaa
    98161 tgggattgaa ggtatattaa acaaaaatat aatagattca gactccttaa acaaagataa
    98221 tgtattaaat ttaaagcaaa caccttcgtc tggtcttggt tcttgtagat acaaactggc
    98281 tgattataaa ataaattctt atgattatga tagagttata aatatactag atgaatatga
    98341 aatagataca ttattttata taggcggaaa tgattctatg gacacagtga tgaaattttc
    98401 tcaatactgc aaaaacacta cttgtaaaat aaaatttata ggtattccta aaacaataga
    98461 taatgattta ttaggaactg acaactgtcc tggatttggt tctgctgcaa agcttatttc
    98521 tacactaata atggaaacat acttagattt ttctatatac aaaaataatg gtatatttat
    98581 agtagaaact atgggtagag atactggatg gttagcagct tcttcttcac tagctagact
    98641 taatgatact ccacttgttg atttaatata ccttccagaa attgatttct atgaagataa
    98701 gtttatagaa gatgttagaa aaatatttaa agaaaaaaat aaggcttata tagttgcgtc
    98761 agaaggtata agggataaat acggcaactt tatatgcgaa caaaaaactt ctgctcatga
    98821 taaatttggt cattcccaat taggtggagt aagcaactat ctaaagaatc ttatagtatc
    98881 atcatcaatt acaacaagag taaaaacctt agaacttggt gtaatacaaa gatgtgctat
    98941 gcatttagct tcagatactg atttaaaaca agcagctgaa gttggtgcat atgggataga
    99001 actatctaaa aaaggatata caggagttat ggctatcatt aatagaacat gcaataatcc
    99061 ttataagtat gaagttagtc atactgatat ttctaaaata gctaacaaga caaaatattt
    99121 tcctaaagag tggataaatg aagaatgtaa ttatataact gatgaagccc taaattatct
    99181 atatccactt attcaaggta aaccagatat caaatataaa aatggactac cttatttttc
    99241 aattatttac taggaaatta ttataatttg atatacactc aattctatcg ctcttaaata
    99301 cctaagtggc tgttaaataa aattataaat tgttatatga cagtcactta atcgtttaat
    99361 tatttaactg tttagttttt taatttttta atctttttta atactaatac gatttgttgt
    99421 aataatatct cacacccatt ttcattttgg atagattata attagttaga caataaatat
    99481 tttgtcatga tattattata tatagtgata caaatagaaa tacttttatc atggctagaa
    99541 aaacaaagat tacacaaagg atttaaaaat caaacaactt caattaaaat aaatggttaa
    99601 aataacttct aaatcaacta ctataaacaa taaaagaaca taaagtagtt aagtctacaa
    99661 ttataaaatt agtaagacta taataactta aataaaatta aagttaacaa agactattaa
    99721 agttagcaaa tattcctcta cctaaataat tgctttttat aaagagttat ttattttaat
    99781 tgaatttaat tgaaatacga ttttaactag aacaatagat taatgtaata tttaatataa
    99841 aataattata ataattgaaa aggaagtaat ctatgatgaa aatagcagtc ataacaaccg
    99901 aatttttaaa agagtttgta gataattcta taaaaaagtt aaatattaat gcagaaattg
    99961 aaatatatat ctatagagat ttttctcatg ttggagattt atatttagaa atagaagata
   100021 ggtttgatgg atttgcagtt agtggcccca tccccaaaaa agctattaca aaaaaggctg
   100081 gaactattaa aaaacctcta gttgattttg gtactgactt acaaagttac tatgatatgt
   100141 ttctaaagct tatctttaaa tataatacat tagatttttc tagagcgtac tttgacttaa
   100201 cagactgggc agataatcca aaagatatat catattattt atcaaatggc acatttggag
   100261 acttaatgga tagtattgat gaaaatgcat ctaaaatgag tttatctgac atagcaaaac
   100321 ttgaaaaaaa gattgcaaaa aaacatatta atctatggaa tgaagggaaa atcgattttt
   100381 ctactactcg ttttagtagt attgttccta tgttacaaaa tgcaggagta aactttcatt
   100441 tcatataccc tgatatagat ttattaaaaa atacttttaa taacttaata aaagatattc
   100501 atttacatag aatgaaggga aatcaaccaa gtgtaatata tataacagtt aaagatacaa
   100561 attatgatga ctctactcta gaccaagttt ataaaatatt aaatagtatt aaaaaagata
   100621 aattaaccga ttttataatt gaaaaaaata gtaattcttt taaaattttc actaaatgtg
   100681 aaacgattaa atcattaact aaaaatttta ctgcatgtta tataaaagaa tctattgaaa
   100741 atagtacaaa tattaaagta tgtgttggat atggaattgg taatgatata aagcaagcta
   100801 tgtctagtgc cataagtgct aataaggaat ctaaaataaa tcatttaaat tacagtttct
   100861 tagtcaacga aaattatgag acaataggac ctttactgtg tgataagtta cttactgttt
   100921 caaataaagt aactccacat ataaatacaa ttgccaaaag ttctaaattg tctactctta
   100981 ctattcaaaa gattatatct gttactgaaa ttctacaaag agatgaagtt actcctaagg
   101041 aattatcttc atgtttaaat gtaacagtta gaactgctaa tagatttcta agtagtctct
   101101 taaagtctgg aaacgctact atattatatg aaaaacaaaa ctcaacaaaa ggtagacctg
   101161 aaagagtata caaattattt atatcagaaa ataaatatta aaatccaaaa tgcataaaaa
   101221 tattttatca tctaatatta agtaaaatta tatatgactt tctaaaaggt atatctttgc
   101281 atctttttaa gatatacctt ttataatatt taataatatt ttatgttatt atctactaaa
   101341 attattttct aaccatttta ctgcacaatg agcaaataaa gctgaaccat aaactaatcc
   101401 ctcttcatta aaaattgctt taggattatg cataccaaaa ttatatccct gactaatact
   101461 tcccattcca atccataaaa atactcctgg aacccttagt aaaatctctg aaaaatcttc
   101521 tgaacccatt gcagatggaa agtctattac attttcattt cctataactt ctttaacata
   101581 agatacaatc tcatctccta catccaaatc atttataact gcaggagtcc ccattttaaa
   101641 ttccacttta gcatttcctc caaatgattt agctattcct gtagatattt caataattct
   101701 attttttaat ttttctctaa cagattggtc aaatgttctt aaagtacctg acatagatgc
   101761 tttatttggt atagcatttg gtacgcttcc tgcattaaac ataccaatag ttaaaactac
   101821 aggctctttc tgaggttttt ctcttgaaag tatagtgttt aatgctattt gtatatgtga
   101881 cattatgttt attgggtcca ctccaaattc agggcttgct ccatgagttg cttttccttc
   101941 tactgttatt tcaaatatat ctgcagatgc catagctgga ccacgtgaat aagctacttg
   102001 acctgtgtta agaaatttac ccaccatact gtgaaatgcc atagctgcat cgacctgagg
   102061 attttctaat ataccatcat caatcattga ttttgcacct ctggtagtct cttcatctgg
   102121 ttcaaataca aactttactc tacctttaag taagtgctta ttatccataa gtattttagc
   102181 tgctcctaag agcattgctg tgtgattatc gtgtccacaa ctatgagatg catctttatt
   102241 cttactttta aaaggtaaat ccgtctcctc cataatagga agtgcatcca tatctgccct
   102301 taataataag gtctttccac catttttact gtctaaaaca gctactattc cattattaga
   102361 cgtcaatttc ggagaatatc ccattttttc taaagtactg gtaacaaagg ttactgtttt
   102421 cggtagattg caaccaattt cgggattttc atgtaaatat cttctccatc taataatttc
   102481 cccttctatt gactgcgctt ctttaaaaaa atttttcata ttccttcctc ctaatcagat
   102541 agtcagcaaa atctcacttt cttttagtaa tgaaatgatg ctgatataaa aattataatt
   102601 ttatatatta aatttcatgt ctttttaagg agttggaata tcatctggaa taggacattt
   102661 atatgtttta ccattcatag atttatcaaa ttcttctttt gcctttttag ctatctctgg
   102721 attatttaat attttagctc caaacatagc cataatcttt gatgcatata tcataccttt
   102781 ttctcctata gaacttcctg cacaagatgt aaactgccag ctatgacctg gtgctcctag
   102841 attcgtacac cctgtaaaaa agtatgctgt cggaactata tgcattacat ctccaatatc
   102901 agtagagcca taactattaa agtttgtaac atttcctcca ccataatata gatgagttcc
   102961 ttctggcaaa ttatatttac ttatcatagc cttatattgt tgtgggcttt gtctatctaa
   103021 ttcagctgca aaatccaatt cctctcgagt ccaaggctct tgtttaatct cattcataca
   103081 ttctgataca agatttgcta aaacatgatt gtttaaagta ttataacacc ctcctaaaaa
   103141 ctctacttca acttcagttt cagtcatcat tgcagctcct tttgctacct ttacaagtct
   103201 ttcatatgta ctttctacag cttctctact taaagctctt gtataatacc atactgatgc
   103261 tttatcagga actatgttgg gtgctactcc tccatctgta attgtataat gaatccttac
   103321 atcagaagta acatgttctc tcaaataatt agctcccaca ttagtaagtt ccacagcatc
   103381 tagtgcactt cttccatttt gagggtctga gcctgcatgt gcagtctttc ccttaaagtg
   103441 aaactttaca gagttaacag ctgttgaagt accaattgta gcttcactaa ttttatttgg
   103501 atgaaaattt atagctaagt ctaacccttc aaacgctcca cctctagcca taaatccttt
   103561 acctgttaat tgttcttctc ctggacatgc ataaaatact atagtcccac ttaagttatt
   103621 ttctatcatc tctcttttaa gccctactac tgcccctaca tgtgctgtac ataataaatt
   103681 atgcccacat ccatgaccat atgcacccaa ttcaaatgct tcttgttttg ttgaaacttt
   103741 ttgatttaaa ccaggtaatg catcaaattc tcccataaaa cctataactg gtttacctga
   103801 accaaaacta gctctaatag ccgtggctaa acctcctaca cctacttcca catcaaatcc
   103861 ttcacttctt agaaagtttg ctgtattttc acatgcttta aattctttaa atgctttttc
   103921 tggattttcc catattttct tacttaaatc atcaagtttg aatctttttt tatctatctc
   103981 atttatagat atacttccta tcattttcta ttcctcctat aaatctattt actatcaacc
   104041 aaatataatt ggacctatta tactagcaaa tgccactgat gcaatggtta cagtaacaaa
   104101 tcctcctata atcatctttg gtaataaata atctgttgcc ctttgttttt cttcttcagt
   104161 tccttccata gcatcaatac cttcattgga aattatctgt gttgcaggat aagctagcat
   104221 acaagttaca ccaattgcag ctgacattct tggagaatat cccaatagtt ttcctacaat
   104281 tgcagtaaat agtattataa atacagagcc tacaacaagt attcctatag ttggaataat
   104341 catcccaaac aatgctgttg gagttacctt agcaagtcct ccaggtaaca tcaacataca
   104401 accaaacatt ataattccat tagattgaga tttatctaaa attccttttt ctaaaaaacc
   104461 tattcgtgta aaaatcaacc ctactaacaa acatgctatg ttgggattta aataatagtt
   104521 tgcaggggta aatcctggta ttaaagtagc caatcctaca aaattaccaa tcatagcaac
   104581 aaatgaaagc tttgcaagat atactaaagt agatgatttt ttacctaaat cttctttaaa
   104641 aattctcata cttggaagtt taaaattact ttttgactca tctttcaaaa aatccccgtt
   104701 atctctttta aattttaatt ctttatttat acaatatgta gttatcggaa tgccaaagaa
   104761 cttttgaaat gataatacta gaattacaaa tgcagcaagt tcaggtctat ttgcagcttc
   104821 tgcaacagtt gttacgataa taccagcaac agtagaaccc gctattggag gtgctgctat
   104881 taaagcatac tctctaccaa ataacatact acccactgtt aaacagccaa cccctattcc
   104941 tacaatacct actaatgcaa ttacaaccgt tttccattca tgacataagt cttcaagatt
   105001 tataagtgtt cctaagttag ttattaataa agctatacca aagttgctca ttgcagctaa
   105061 tatccctgat gctcctgtaa tgtcttttgg taaaatattt gtccaaaatc ctagtaaaaa
   105121 tataatacat acaaataaca atgttgaaat tgcacctttt gttttcttag ataaaaactc
   105181 actaaaagca taaatcatta cacatacagt aaagcaagta agtggattcc aaaaattttc
   105241 cattgttgtt tcctcctaaa tataataatt ttttatcaat aaaacaataa ttataaaatg
   105301 tatattatgc agttattctt aactacatat ggtatagctt gatatttttt gatgatatta
   105361 tatacgtcct aaattattcc taaaatatct aaataaatat aactagagtt aactctctaa
   105421 atattaacta tagttatcaa cttactagat ttctttttga tttttacttt ctacataatt
   105481 ttacgagtta gaattgtact tcaactattc tctatattag tatacatgtt ggaagactta
   105541 tatttattta aatattagat atagaatata tttttataaa cgtcttaaat tattcttaaa
   105601 atatctaaaa cgaaataata atatacccat taaaccaata ttatcagaat atacttcact
   105661 ttaagtcttc tttatataga aattttatct attagtgcat cccctccctt gtaaattaga
   105721 ctatttctaa gcgaatttag atatcttatt cctagagact attactaaaa acaaaaatta
   105781 tttattatat taaatcaatg ataagccata ctttaaaatt ctttctatac aagtataatt
   105841 tatcatataa tgacatctat acctttatct ttatagtagt cctaaattat tcttaaattg
   105901 acaataaaaa taatttcact atttataaaa atacttgttt ttatattgtc aaactattct
   105961 taatatagat tataatttta aatcttttct ttgtcaatat attctgacat ttttccagaa
   106021 aatttataaa gttatatagt gagtaaatca tactattttt atataatgag taaattatac
   106081 tatgaatatg tctaaatatt tacttaagta taaaaataaa aaatgtgtat tctccttttt
   106141 atagtaattt taattgccat tatttttaag aagaatacac actttacttt aaagacccta
   106201 tactctattt taaatgcttt actagacact aattaacatt agttcctaac ttctatttat
   106261 cttattccta ccctactact atatccgtag aaaaatctaa aacttctcca ttagatttta
   106321 ttacttcttt gtaccaatca aatgattttt tcttatatct cttaaaactt cctttgcctt
   106381 catcatctaa gtcaacatag ataaatccat atctcttact catctgacct gtagttgcac
   106441 tgactaaatc aattggtccc cacatcaaat atccaaaaca ttcaacatta tcttcttcaa
   106501 ttgcagactt catttgagat agatgcttac ttaaatattc aattctataa tcatcattta
   106561 tagttccatc ttcttcgatt ttatcatatg cccctaaacc attttctgta attaagatag
   106621 gtaattgata ctttctgtaa atatagttca aggtaaatct taaacctata gggtctattg
   106681 cccaccccca cttactttgt tccaaatatg gattttgaat cccaccaaat aaagatttat
   106741 cacctgataa attttctttt ggagtagata cacttgaaaa ataataatta agtcctataa
   106801 aatctaactt accttcttta aatgcttttt tgtcttcatc agtcacttca atagttatat
   106861 tatttttttc atattctttt aatttatatt gtggaaactt cccattgcac attgcatcaa
   106921 tttgataata atcacggtca ttatctaaaa atgcatttaa cacattacta ggattacaat
   106981 tgtatggata aattggattt agtccaaata cacatccaat tttattgttg ggattgattt
   107041 ctctagccaa ctttgcaacc tttacacccg ccaaagtcat attataccct acatttgcca
   107101 atgtttgaac cttattttct atttcactat acttaatacc tgctatcata taagtaaaaa
   107161 tatccgagtg ctctgtttga gggtcaatat ggttcatctc attaaatgta acccagtact
   107221 ttaccttatc atcaaatctt cttattacag tctcactgta ctttaaatac aaatctataa
   107281 gttttctgtt attccatgaa ccatatttat gtactaaatt cataggtaat tcaaaatgat
   107341 atagtgttac aataggttct atattatttt ctattaatgt atctatcaga ccttcatagt
   107401 atttaatccc taattcatta ggattttcat catctccatt tggaaaaatt cttgaccaat
   107461 ctatagatat tcttaatgca ctaaatccca tttctttaaa taaagctata tcttctttat
   107521 atctattata aaaatctatt ccattatgag atggataatc taatttttct tctattttat
   107581 cagtaattac tcttggcgtt tcataagagc ccttagttac aaagtccata attgcaggtc
   107641 ctttgttatc actatcccat gaaccttcac attgatgggc tgcaatactt cctccccaga
   107701 aaaatgtatc tttaatattc atgaattttc cctcctaaaa tttatactat tacagttaat
   107761 aattcttctg attcatcatt tgatgagatt acatctaaat aatcgctagt atttgtaact
   107821 acagttatta tagtagaatc aaagccttcc tctataattt tatcagattc aaatactgct
   107881 aaagcatctc cttttttaac cctttgattt tgtgttacaa gactttcaaa gaattttcca
   107941 tctaagttta cagtatctat cccaatatga attaatattt ctaatccttc atcagtcact
   108001 ataccatatg catgtttagt tggaaatact atagttatag ttccatctac aggagatata
   108061 acagttgatt ttgattcact aggtataact ccaacccctt tacctaatgc tccagaggaa
   108121 aatgctttgt cattaacttc cccaagtgca attacctgac cttttgcaac atttccaata
   108181 ctaatagttc ttgaattgtt ctcgttgatt attagttcta attctttttc attatcaccc
   108241 ttaacttctt ctttttcatc tgaaggtatt cctagtaaat aggaagctgc tgttgctgct
   108301 acaaatgata ctactacact tattatcata aatgtaaagt tagatacctc tcctccaaca
   108361 agatacgttg gtaatgcagc taatccccaa accattccat atgttttaac ttgagctaaa
   108421 cctgctatta aaccacctat acctccacct atcattactg ctacaaatgg tcttctatat
   108481 ttcataaaca caccaaacac tgaaggttct gtaactccca taagtgcact aaaacttact
   108541 gtaccaaata gttgtttttg ttctttattc ctagttttta agaaatatcc aaacattgca
   108601 ccacaaactg caatatcaga tattgttgca gcgcccataa aaattgggtc ataccccaat
   108661 gttgtcacag aatttaaaga tataggcata gtaaagtttg ctgcaccaaa aactattaag
   108721 agtggttgta aagctgcata cagtgcaaca acactccaat tacccaatgt atcaattaaa
   108781 aacatacatg catagtttaa tccatcacca atccacatac caataggtcc aaatacagtt
   108841 aatgtaactg atagagttat tattaaagat aacactggaa ctaaaaaata gtgtaatgct
   108901 ttaggtaata tttttttcaa accttcaatt ataattccca ttgcccatac tcctaaaagt
   108961 attggaataa aactgtttgc ataagttgta gcctgtaaat ttataccaaa taaacttaat
   109021 ccttctacac catctattga tgaagataat aacgtaactg ctaacactac tgctatatag
   109081 ggatttacat taagtctttt tgctgatgct attgcaatta atactggtaa gaagtgaaat
   109141 gtagcacttc ttaatgtatc taatatttga tatgtaggac tgtcactctg aagtatgcca
   109201 gacatattta aaagtgctaa tatacttgca agtataccag ctgcaagtag tacttcaatt
   109261 actgaagtca tagtctctga gactaccaca aagatggaat taaatatacc tttgatacct
   109321 ctaattccct tttttctttc actttttata cttgattcac ttgaacttaa acctatcata
   109381 gacataaact cgttatatac atctacaaca tgagtaccta ttataatttg ataagcaaca
   109441 tcattagaaa ctacatcaat tactcctttt aattttttaa tttcttctgt ttgagcgata
   109501 ctcctatcat ttaaagtaaa tcttaaacga gtaacacaat gcgctaccga ctttatattt
   109561 tcttttcccc ctataagttt tattatattt tcatttaaat ctttatattt catctgattt
   109621 tgcctccagt ctttgagtca ccctatttat atgcaacatt aaataagttt cttcacttat
   109681 agatatatcg acattaaatt gtgattttat atattttctt atcttttgaa cacttccaaa
   109741 tgactttgga taaagttgac ttacttgttt atataaatct atagaattat cttcatctat
   109801 tgaagcttca tccgacataa ttctttggat aaagtactgt agatgagtag taaatctcat
   109861 ataatttgtt gaagtttcat ctagctctac attaaaatga tattttatta tagaaactat
   109921 atctgctaaa gttctcaact ctacaattgt actatcatga gactctttat ttacctccat
   109981 atttacaaaa tgaagtgcta ttgatactgc ctcatcatca ggaagattaa ttcccttact
   110041 atcaccaatt aattttagtg catgcattcc aatttcaaaa tgctttggat aaaatctttt
   110101 aacattccat accaaaggac tttttataaa ttgattcttt tcacatcttt taagtaaaaa
   110161 ctctaaatgg tctagtaagg ttaagcttag ataatcactt gcctttatat ttaaagcttt
   110221 ctcaccatat tcaattattt cattaataag aactatatta ttggagtctg agttttgtag
   110281 tagataacta aagtgttcta gcatatcgta tgaatctagt atatatgttc tctctatttc
   110341 attttcattt attacttgtc ccactctttt tttaaagcca attcctttaa caaatacaat
   110401 aatttcattt ttacctcttt ttactaaggc tacattatta tttaaaatct gaattatttt
   110461 catatattcc tcctttcaat aaaaagaact caagcatata tgtatagagt atccctctat
   110521 ttttcacata tatacttgag ttctgcctgc taatcagtaa catactcaaa attatgacta
   110581 cgtcgccata attaaaattc ttttttgttt aatttcatat tctatatgtt atactatcca
   110641 tataaatctg tcaacggttt cctagatatt tttttattct ataactacaa gctctgttat
   110701 gcgattatct gacaaggttt atatactaaa ttgatactat tttaccgtca ctaatattta
   110761 taatattgtc tgaaccatta tctattttac tattatgaat tatcatagta atgcttttaa
   110821 atataatgaa ctgtatgtta acaaataaat tcttaagata tattaacata taaagtctta
   110881 agtttagttt tatttaatca ctattaagat atattaatag gtaaagcttc aaatttagtt
   110941 tcatttaaat gctagtaaga tatactaata tataaatact taaatttagt tttatttaaa
   111001 cgctctagtg atatttttaa ataaatttta ataaaaatca ttctattttg tcataaatca
   111061 tttatctggc ttatatttaa gatatattag tggatacttt acacttttta attcctcatt
   111121 acttcttaaa agtttattat tattttccat atcataaatt tgtaacatta ctttatttac
   111181 atatagagca taataattat cactataaaa atattgatac tcatatcgag ggtcatcttt
   111241 gtaatacact ttgtatagta ttccttcgta acttgccttc ttggcttttt cttttgtaat
   111301 actttttata ttatcttttg aaatatcttg ttttaccata tacttatcaa tatatgtttc
   111361 agcatctttc catggtttaa tgcagttttt atatacaaca aaccctgtta cacttataat
   111421 acctataatt aatactataa aaataacttt atattttttc atattaatcc tccttcattt
   111481 attatttaaa actcgatata atgcactaaa aaaaggatgt tatctttttt attaaaatat
   111541 aacatccctc atatttagca tcaatatata tgccccaact ggtaggttaa aagaccccac
   111601 tgtcagtact ttgattaaat tacatgtatg tatatatatt tttaacaaat ctattatatg
   111661 tcaacttgtc tactattaca tataatctgt aatattacat ctgctgcttt ttttgagcct
   111721 cctgcattat aaaagctttt tgatagttta gttgcatttt ctttatattc attgtcattt
   111781 ataacttgaa atactgcttt tttaatactt tctgacttat cctcttttaa cataattcct
   111841 gctcccaaat gaacaactct cttagctacc attctctgct ccgaatgttg aggaaataat
   111901 accataggta ctccataata taaactttca ttaacactat tcatcccaga atgagtaaca
   111961 aacacatcag ttttttgaag tatagcaatc tgttcaacac tatttttgac ctcaaaatta
   112021 tttggtatat ttcctaaact tttaatatca gtacttctac caacagacat aatcacatca
   112081 acatcagaat ctttaaatgc acttatacag tttttataaa agctaacatt tttattatta
   112141 attgtaccaa gcgaaatata tattagcttt ctttttctct ctttaggttc aataatcaac
   112201 tttgatactg atggacctac aaatgaatac ttactagaga aggtttctac cattggttga
   112261 aattcttttg atgtatatac aatggtatcc gtatcattat cattagaaac tatagaaata
   112321 aaatttttta cttcataccc ttttctttgt aaaagcttta tttttttatt aatgcgtctt
   112381 acaccaaaaa tcatacgtat aatttctata aagttttgtt tcatcaactt tgctgtatgt
   112441 ttattaaatg caaaagttgt tgtagagcat acatatggaa tattaagttt ttgtgcaaat
   112501 aactttcccc aaaaacttaa tgaatctgat acaatgcaat ctggattaaa atctttaagt
   112561 tctctacaaa ctttttcatc taaagaaata gtagtatcta ctatcatctc aattaatcca
   112621 ggaaaatcct taccaatttt tttctcatct ccagggagta attctggaag gtatttatca
   112681 caacaaataa attttgctcc agcaccttca attttatctt taaattcatt aaaagaataa
   112741 tacaatacct catttcctct atccactaat tcacgaacta cttcaatagt aggattagta
   112801 tgaccatatg caggaatgct aaaaaatact attttactca tatttcatac ctccttttat
   112861 aattttttta aatcattctt ctttacgtac tactcccaca tgacctataa aatttatcaa
   112921 atcacgttga tgaacaattc ctaatcctct ttcctcatct ccaagtacca ataatttatc
   112981 tcctgcattt atattaaata cttctcttgc ttcttttgga atgacaatct gaccacgttc
   113041 accaactaca actgctccaa agaaaaactt tcctttagga ggtactataa tacctgtttt
   113101 ttcttctgaa tgattaacta agtcatctag tttgacattg tacaattttg caagtttaat
   113161 gcaactacat atatcaggaa tagattctcc actttcccat tttgctattg attgtctaga
   113221 aacattaagt ttttcagcta attcttcttg tgtatactgg ttgatttttc ttagactttt
   113281 taaattctca ttaatcatat ttttcatacc tcctatgttt ataatattgg atttattatt
   113341 taaattctat caactaatct taacattcat atatatattt atatactttt attatataaa
   113401 tttattattc aggtaacata tttccttaag ttatcgtaaa caaaaaaaca agcacaaatc
   113461 tactaactgt tgttaaagta tttaagttaa ggtcatttac attatcaaat atttctacac
   113521 ctttaaaata acctttatat ttataatctt tcataagcaa attattatcc cttctcatat
   113581 acaagtccag ttgatgtctt gttgctaaat ttaatggtaa tcaaattctt taccaataag
   113641 tataaaaatt actaatgtgt tataattttc ttaactacta aattttacat acatttgaat
   113701 agaatatatc aataaattac tataataaat aaaaaatagt tagggagata ttattatgat
   113761 tttatatttt agcggaactg gtaatagccg atatgtggct cgtaaaattg cacaagaatt
   113821 aaatgatgaa ctgatatcac taaatcaact tataaaagag gaaaaaacag atgaattaat
   113881 atcagcagat aaaccattca tatttgtatg tcctacttat gcatggagat tgccaatagt
   113941 agttacggat tttataaaag aaacaaaatt tctaggaagc aacagagttt actttgtaat
   114001 gacttgtggt ggagatacag caaaatccat aaactatatt caaaaactat gtaaatacaa
   114061 ggaatggcaa ttaaaaggta tggcagaaat taaaatgcca gagaattata tagctttatt
   114121 ttctacaaca gacaaagaaa catcaaaaca aatgattaaa gaagcagata aacaaatata
   114181 taggattatt tctgatatac gcaatgaaaa tgaatttgaa actattactc ctagtggctt
   114241 aggaggtaca attaaaagtg gtataataaa tacagttttt tataagacaa ttattagtgc
   114301 caaaggattt cattatactg ataaatgcat tggctgtgga aaatgtgtag agctatgtcc
   114361 tttaaataat attaatttaa aaaacaaaaa accagtttgg aaaaataact gtactcattg
   114421 tatggcatgt atttgtggtt gccccactga agctattgag tataaaaata aaactcaaaa
   114481 cagagaaaga tactatttag aataatatat tcttaatatt taaaccatta taaaatgaac
   114541 tatacccatt gttttataca gttttacata ttttaaccgt ctcctaaaat gggtatagtt
   114601 caaaagatag tgtctcaaag tgaatttttt agttcctaaa tcaatctttt tgtattaaat
   114661 caagtataat ttaatcacct tttcttatat cattttcact aatatgttgt attccaacaa
   114721 atataagatt tattagctct gttagtgact tccattgtga tttttttgaa tcaagataat
   114781 aataattttt cgttccttct ctacgcaaat taacaatctc tgcttctttt aatatcttaa
   114841 gatgatgaga tacagcagga cgagaaagat gtgttttttt tgtgatttct cccactcgta
   114901 taccattaaa atcactctct aaaagagcaa ttaaaatcaa ttgtctagtc tcatcaccca
   114961 tagcagagat agctttgcga cattctttaa atttagacgt tatttcttgc attctttgtt
   115021 gacattcttt ttccataaaa tccaccctct cttataagta tctaacagtt gctacttctt
   115081 tctgtgcagt acgtctatat ttaacatctg ggtatccaat tactaatgtt gtaagaacat
   115141 gattactacg ttttagacct aaaagctttt ttaattttgg cgaattattt gctacatctg
   115201 aaaaatatcc actaaataaa acacctagac cataagactc tgccattaat gccatatttg
   115261 atgctgcaag tgaaccacta atcttatctt ttgtaattat cattattgca ataggagcat
   115321 gcttaaagaa aaagttatcg tcaatctcaa cttcttttga atatttaata gctatatttg
   115381 caataggttt tatttttcta aaaaatttca ctgcttcgtt ttcataaatt gactttttat
   115441 tgtctaaaac aatatatgat acatcttgtg aatttttagc actaggggta tacctacctg
   115501 cctcaataat ttgctttata atttctgaag aaacttcttt atctttaaat ttacgtatac
   115561 ttcttctaga tttaattgcc attagtaatt catctgaatc tagctttggc ttatttgtaa
   115621 gttctatagg aggttcatca aatcctgtca aggtaatagc ttttgtggga catattgctg
   115681 cacaatgtcc acacattaga caatcttgtt ttttaattac tgacttttta ttttctataa
   115741 ttatattgtt tactggacaa tcatttttac aaagcccaca tccaatgcaa agttctttgt
   115801 caacttctat tatatgttgg tatttcatat tcattcatct acctttcgtt tattagttta
   115861 aactattata acacgtagac attttttata ttcattggta aagaatttga ttaccattaa
   115921 atttttaatt tctctattaa ataaattttt catagaattt taaaatatca tagtttatac
   115981 ataataaaaa agacaagctt aaaacctaag cttgcctttt tcatctttta ttacaaagta
   116041 aattgcactt acttcccata cttcttaaca attttatttt ctaaaatttt atttgtgaat
   116101 catactattg catcaataca atgtaaaata attctatatt gattttctat agttaaaaaa
   116161 gttaaaaatt atacttatta ttaattttta tactcttctt atttaataca aagttcttat
   116221 ttaacataga tttcttgtct aatatagatt tcttatttaa catagatttc ttgtctaata
   116281 taaatcttca ccatttgaag caattacttt cttataccat tcaaaacttt tctttttcat
   116341 tcttcttaga cttccattac cactattgtc tctatctaca tatataaatc cataacgctt
   116401 ttccatttca cctgtaccag cacttactaa atcaataggt gcccatgtag tataacctaa
   116461 taaatcgacc ccatctatag aaactgcatc atgcatagct tttatatgtt ctcttagata
   116521 atcaattctg taatcatctt ctacataacc attttcatca gctatatctt tagcacctaa
   116581 tccattttct acaataaata gtggcttttc atatctatca tatacatgat ttagtgtaat
   116641 tctcaaacct aatgggtcaa ttggccatcc ccattgactg tatgaaagat atggattttt
   116701 agcagatgta aatatgtttc cttcagccat atcatctgca ttttcatcgg ttctaacaca
   116761 acggctattg taatatgaaa atgaaataaa atctactgta tatttattca ataattcaat
   116821 atcaccaggt tccatttctg gtgtaatacc tttttgctcc atatacttta acgcatagtt
   116881 aggatattta cctttagatt gtacatccac aaagaagtaa ttttctctat ctaactttgt
   116941 agcttcccat acatcattag gattacaact ataaggatat acacttccag ctgcaaacat
   117001 acatccaatt aagttatttg aatcaacttc atgtgctatt ttagttgcaa ttgcactagc
   117061 tacaagctca tgatgggcag cttgatattt tatcttttca acatcttcac cttcttcaaa
   117121 gcaaatacca gcacccataa atggagcatg taaaatcata tttatttcat tgaaagttag
   117181 ccagtacttc actaatcctt tatatcttct aaagataaca ttgcataatt tctcatagaa
   117241 acctatcatt tttcgattac gccatgcacc atacttttta actaaatgca ttggacaatc
   117301 aaagtgtgta atagtgacta aaggctctat tttatatttt ttacactcct taaatatatt
   117361 ttcataaaat tgaagtccct tttcatttgg tgtttcttca tcaccttttg gaaatattcg
   117421 actccatgca atgctcattc tatatacttt aaatcccatt tcagcaaata actttatatc
   117481 ttctttataa ttatgataca tatcaatacc agttttagct ggataataat gctcatcatc
   117541 aaaatcaaac atttttagtt tccctgtaat aactgctgtt ctatcttttc ctgttggaca
   117601 tacatctaca tttgccaatc ctctttcgtc ttcattgtat ccaccttcac attgattggc
   117661 cgcagtagct cctccccata aaaaatcatt cttaaaactc atagtcaaag cctcctaaaa
   117721 ttataaccaa agcactagat tatgctaata cctcaatctt tactaaaata agactgtaat
   117781 tagattgtct tctaattcaa tatttttact ttctgtttct actacatcta aaaattgtga
   117841 tgaatttgta ataacaattg gagttacagt actatacccc aactctttta ttgcataaac
   117901 atcaaagttt aatatagttt gaccttttgt aattctatca ccttgtttta tacatgcctc
   117961 aaaacctctt ccctctaatt gaatagtatt taatccaata tgaataagca cttcaacacc
   118021 tgtatcagaa agaatcccta tagcatgtag actaggaaat aaggttgtaa ctaccccatc
   118081 tactggagca actacttttc catcactagg tattattgcc accccttgac ctagtacacc
   118141 agatgcaaat gctgcatcct ctatatcact taattttaat acttcacctt ttataggact
   118201 tgttactatt tctctttcta atttagtaga cttgacttct tttatttctt ctttaccctt
   118261 atttcttact tttttagact cttctacttt ctcatcttta tataaaatca tagttaatac
   118321 aaaagctacg accattgata tagctatacc agaaagagct actattaagt tacccattcc
   118381 cccatctggt tcaatcattg ctggaagttc aaatattccc attcctccag ttataaattt
   118441 tctaagatta aagaatccat agaaagcgcc tccaatacca ctagcaatac aacttattat
   118501 aaatggtttc tttaaaggca ataatattcc ataaattgct ggctctgtaa ctccaaatat
   118561 accagatata aagtttggta ttgccatctc ttttagtttt ttatctttag tcttgaaaaa
   118621 tattgccaac acaactgccg aacttgcaaa tgtacaggca aagaatggca tcataacatt
   118681 atcatatcct aaagtcataa cattgtttat atatacagga ataaatcccc agtgcatacc
   118741 aaaaataaca agaatttgcc aagtaaatcc tactatagca cctgccaata gtggactaaa
   118801 atctcttatt gaaattacaa tttgtgaaat aagtgtagaa ccaaatgttg ctatagggcc
   118861 tataactata agtgcaacag gcactgttat taataatgta agcattggca caaagaaaaa
   118921 cttaactaaa tttggaacac acttattaaa taatttttca cacctagaag caaaatacac
   118981 tataaatata attggtacga ctgttccagt ataatcaatt gatacaattg gtagtccaaa
   119041 gaaatcaata tatactggtg atgcaaacat tgttccttta aataaagtgt acatcacatc
   119101 tgctcctgct gatatactgc taccttgtat agcaggataa catactgcag ctcctagtgc
   119161 taatcccaac attggtttta atccaaactt cttagctgaa gtatatccta agaacaaagg
   119221 aaggaatgta aataatgcat ctcctgctgc atttattacc atataaccac cagatacatc
   119281 actatataat cccattgcta caaataatgc attaaatcct ttcaacatac cacaagctgc
   119341 cattattcct aacactggct gaaaaattcc tgatattata tctatacctt tatctagtat
   119401 attacctgat tttttagttt ctgaacttgt acttgaatca tctaaaccaa ttatttcaac
   119461 tacatcttta tagacagctt caacatgatt tcctataact acttgatatt gtcctccact
   119521 tttcattaca gtaacaatac cttccatgtt ttttaataca ttgtcatttg ctatactttc
   119581 atcttttaaa gtaaaacgta atcttgtaat acagtgtact aacccactaa tattttcttt
   119641 tccaccaaca ttgtttacta tctcttgtgc taattgtttg tattttccca tttttctcct
   119701 ccatatttcg tttaaattaa ttttttgaaa tttgaaggtt tggccaactc aatgttacca
   119761 tccaaccata agtataattt tgttagtatc actcctttca atcattctta atatactact
   119821 gaaaaattat ttcacttaca tatttgagtc tttaactact ttagctaaat gtattgttaa
   119881 atatataagc tcatcatctg ttaatatgta gttatattgt tttttaatga aatttttaat
   119941 tttaactaca cattcaaact ctttattata tttagattta ataatgctta gtaattcttt
   120001 gtctgtctca ccttcatgtg cactatctaa aagcaatctt tgcgcaaaaa actttaaatg
   120061 cattataaat ctataataaa acactgattc ttcatgaaat tctatcctaa aatgatacct
   120121 aactatgcta agtacttctt gtattaactt cgtaatatca gatgccaaag tatgaccttc
   120181 acttaactga gcattcacta tatgaaaggc tataaatcca gcctcatctt ctggtaattt
   120241 tatacctgtt ttgttttcaa taatatctag tgctttcatt ccaatttcaa actcttcttt
   120301 ataaaatctt ttaatttccc aaaggattgc attttttaca tttattcctt ctttcatcct
   120361 ttgaattgca gaatatgtat ggtcacttaa ggatatgtaa atactttcat ttagcttctt
   120421 gtttaacttt atttgtgcgt attctataat ttcacttgat aatttcatgt gttctatagg
   120481 aatattattg agtaattctt gtagtttgtt tgatatttct ttatttgata ttctaaatac
   120541 tttatttact ttagttttat ctattagatt gccttttgat ttttgataac caattccttt
   120601 acccataacg ataacttctt catcattatc atctaatgtg attacaacat tattgtttaa
   120661 tatcttatta attatcatga ttatcctcct tttaagcata aaaaaagctt atccataata
   120721 atgggcatac tccttcctag gatactgcta ttattaatga ataagcttag cttgtactta
   120781 aacaattact acctaattcc aattaatatg ataaaacatc agacatattt tgtcaacttt
   120841 ttatttccat atacttcatc aatcttttat gttgcttttt gtcagttcta ataattattc
   120901 ttattaatat taacttattt ctttgcatca atataaatac aaaacaagtt atagtcaata
   120961 ataataaaaa ttctattaat gtaaatagtt aatattatac ttcctctact acattatctg
   121021 tactttattt aacatttttt tcaactccaa aaacatatgt cataataaat ggagcaacca
   121081 atgctataaa tataactata agtccattta ctatatttga aatccccttt tcccctgata
   121141 atccaagtac tgccattata ctattttgtg gaacatataa agtcacatga gtaattcctg
   121201 caaataaccc tgcgattcct gctcctatag atgctaacat agcaggtttt ttaagtttca
   121261 atgtaactcc ataaagagct ggttcagtta tacccatgat agctgatatc catgcagctg
   121321 atgctaactg tttcatctta ctatctttgc ttcttaatgc tacagctaat gttgcgccac
   121381 cttgcgctaa attagcacaa acttgagata ttaatattag cgattcttgt cctgtagttg
   121441 caaatgctga tatgaatata ggagtcaatg catgatgcat ccctgtcata acgataaatg
   121501 gcattgcagc tgctaataaa caaaccatta taaatcctaa ccttccttgt agtaaattta
   121561 ttatactagc taatccctct cctaagaaat ttcctattgg tccaattact attaatgcta
   121621 ttggcgcact tattaataat actaaagttg gatttaaaat tgtttttaac atgctaggtg
   121681 ttaatttatc aatgaatttt tctatatatg acatcatcca tactgttaaa agtgcaggga
   121741 taacagatgc tgcatatata acatcttgaa ttggtactcc tataaatgat atagggtccc
   121801 cacttgatac ccatgcagag aaatttggat gtaaaaacac acctactact ataactgcta
   121861 ttgatgtatt aacattaaat tttttagctg catttattgc aagtattata ggcataaagt
   121921 aaaatgctac atctcctatt atatctaata ctctatatgt acttgaattt gatgatagcc
   121981 aattaaaagt ggtgcaaaga gataatataa cttttatcat cccagatgct gtaagggctg
   122041 gtatcatagg tgtcatacaa ccagttattg tatctattat attatttaaa atactatttt
   122101 ttttattttt attttttata tcgcttttaa tatctcctgt ggtatttatc cccttattta
   122161 ttattgcatt aaatacaaca cctacctcat tacctattat gacctgatat tgagttgaag
   122221 ttgagtttac tccaactacc ttatctatag attttaattt atctgcattg actttctttt
   122281 catcttttaa gatgaacctt aatcgtgtca tacaatgagt taagtcaatt atattatcct
   122341 ttcctccaag tccatcaatt atcaattgag ctacttcttc atatttattc ttcatacctt
   122401 atcctctctt ttaaaataat ttttaatata attttaatcc aaattaatct ttttactcca
   122461 agcttgtaat agtgtattgt aatttaataa gctagtatct tctacaatat ggtcacattt
   122521 tcctagtact tcttttttcc ccacaccaat tgatgtcata ttagcagcat ttatagcttt
   122581 aatacctgaa taagaatctt ctattcctac acaatcatat tgttttaaat ttagaatctt
   122641 gctcccttca ataaaaatat caggatatgg ttttcccctt tttaataaag atggatttac
   122701 tatacaatca aattcatctt ttattcccaa agattctaat atcctaggtg cattcttact
   122761 agcagatgct attcccaatt tcacattatt ttttttaagt tcttttataa gctcatttat
   122821 ccctggtaat atatctttat atgttaatga gtttattaac tttatataaa tgttattttt
   122881 ttcttcacac acctttaact tttcctcttt agtataacta tctactttat tagcagataa
   122941 caatatttta tctaatgatt cttccctgct tatgcctttt aatttttcat taaaagtttc
   123001 atctatttct atatctagcg attttgctat ctcatgccaa gcttcaaaat gatacttagc
   123061 agtatctgtt ataactccat caagatcaaa taagactgct ttcatattaa ttactccctt
   123121 taaaacaatt attaacttct attatgttct ttgataattt tatttcttta ttatcatgta
   123181 atatggttat atttctacct ttgattaact tataataaga tttatattct tctatattta
   123241 cagaaactaa attatctcta aataatatat taaatttaat atttttccag tgtttaggta
   123301 tccttggttc aaaacttaat ttatcatttt ttattttcat tcctgcaaat ccatatacta
   123361 aactcatcca tgttccaccc atattagcag catgtattcc atcacttgta tttttttgta
   123421 ggttattaat atccattaat gctgtgtcca taaaatagtt ataagctttt tcatgatttc
   123481 caatttcact tgccatcatt ccaaatattg accttgacaa agatgaatca tgagtagtta
   123541 ttttttcata gtattcaaaa tctctcttct tttgttctaa gctaaactca tcagggaaaa
   123601 gcaacgctga aagtacagta tctgcttgtt tatttacttg atatctatat attgttaatg
   123661 gatgataatg aagaagtaat ggatattttt cttttggtgt atttttaaaa tcccatactg
   123721 atttttctaa aaatgaatcg tcttgtttgc ttattttctt ctcttcatca tatggtaaat
   123781 acatattttg agcggcattt atccagtttt tcacttcata ttcttgtaat ttaacttttc
   123841 taaatacttc atttgaaaat aaattatgct taaatttatc taccatctgt actgcatata
   123901 ttaaattata cttagccatt aaattagtgt agtaattatt gtttactatt gctgtatatt
   123961 catctggtcc tgttactcca tttatacaga atttattatt ttttgattct atataatctc
   124021 caaattcaac ccaaaatctt gctgtttcta taagaatttc taagccattt tgcatcataa
   124081 attcttcatc attagttgct tcaaaatacg ttctaatacc ataagctata tctgcattta
   124141 tatgaaactg tgctgttcct gctggataat atgcactgca ttcattacca tttatagttc
   124201 tccatgaata taagcaacct ttatttattc caagttctct agctctttct cttgatgcat
   124261 ctaagattga gtatctataa tctaataaag ctctggcaat ggatggttga gtatatataa
   124321 aaaatggtaa tatatacatt tctgtatccc agaaataatg cccctcatag ccatcacctg
   124381 taagaccttt tgctgatata ttagtctttc catctcttcc tactgattga taaatatgaa
   124441 ataaattggt ttttatcccc agttgaagag ttttatcgcc ttcaatctct acatcagagg
   124501 ttgtccaaaa ctcattcatt ttctttctct gaatattttt gactttatca aatccaatat
   124561 tacatgcttc tcttaaaacc aattctaatg attttaaatg tgcttctgat tctaaacatt
   124621 ttaacgactc cagacattct aatgatttta aacgttcttc atttagattt aaatcagaat
   124681 atactctact atagcctaca acaatttcta aatttaacct ttcatctttt ttggcataga
   124741 cttctatttc tcgctcaata cattcatctg aaacattata ggtctcattt gtaatatttc
   124801 cacaagtaat atatgaatca aacccacacc ataaccctaa tctacttctt tctgtaacta
   124861 aattcataaa atacttattg ttatattttt ttacattaat tttaaattgc ttcttattta
   124921 tagatgccac tcttgggtcc tctacttttt cacaattaaa cttctccata ctattttcca
   124981 ttttattttt aaattttatt tttccattaa aattcaatgg tgttattgta tatgaaatca
   125041 tcattaattc ttctatatct tgtgatacta atctttctat agatacttta acttttctac
   125101 cacgagagtt ttcccatata aatgaacgtt taagaatccc ttcatttaaa tctagtatcc
   125161 tagaatgttc acttgtaata ccttttgata aatcaaagca atctccttca atagagaaat
   125221 ttactatatg agcattaggt aacttacaga tagtctgatt ttgttttgca tagccataag
   125281 cgctttctcc atatgtaatt ggagattctt cataaaatcc atttacatat gtacccatat
   125341 tatctacata ctcattatta aaatcagact cttcaatatt ccctctaact cctaaatgtc
   125401 cattagctag tgtaaacagc gtttcattgg attttattgt atccttacta aacccttgct
   125461 ctataagttg gtccttagta gtaaatatta aactcataat aacactcctt tcttaaaatg
   125521 aattatgcat gagtttccat gtcattaaat aaaatttctc catctgtttc tattactttt
   125581 ttataccaaa aagcactttg ttttttagtt cttttaaatg agcctcttcc atagtcatca
   125641 gaatcaacat gaattatgcc atatcgtttt gtcatctttc cttcacttac actggttaca
   125701 tctatacatc cccacataag atatcctata agctctattc catcttcatt tactccaata
   125761 atcatatttt taatatgttc tttcatgtaa tctatacgat aatcatcaat tatttttcca
   125821 tcttcattta caatatcttg agtacccagt ccattttcta ctataaataa aggtaactga
   125881 tacctttcat ataactgatt catagttatt cttaacccta cagggtcgtt ttgccataca
   125941 ctatttgatt ttggaagata tggatttttt atggtttctg gtccgtttgc ctttgatttt
   126001 tctaaacctt cagctctagc tgaactacaa gcactagaat aatagctaaa agatacaaaa
   126061 tctacagtat tatccttaag tgttgataaa tcttcctctt ttattattaa ttttatttga
   126121 tttgatttta aataataatt cataaagctt ggatacacac ccctaacttg aacatcagaa
   126181 aaaaacaaat tatgcctttc atgctctaaa gttgcaaata catcttctgg tttacatgtg
   126241 tatggatatg tttttccagc agctaacata cacccaaact taaattcttt atttatatac
   126301 cttcctattt ctatagtttt tgcattagct aataattgat ggtgtgctgc ttgatatttt
   126361 ttctgaagtt gattttcatc agctataaaa gttaacccag cacctatata tggaatatgc
   126421 attatcatat tcatttcgtt aaaaggaatc cagtatttga catcatcttt atatcttttc
   126481 atcaaaattt cagcatattt tacatataaa ttaaccactc ttctatcagc ccacccagaa
   126541 tatttttcaa ctagagaata aggtatatca aagtggctta tcgttattaa tggctctata
   126601 ttatattttt ttaattcttt tattatatta tcataaagtt gtaatccctt ttcatttggt
   126661 tcactttctt ctcctgttgg gaatattctt gaccaagata tagaaaatct aaaacattta
   126721 aaacctattt ctgctaacat tgctatatct tctttgtaat tttcacaaaa tttaatccct
   126781 atatgacttg gatagtattg attattttca tttaaaatat tatcctcatc taaacataca
   126841 tcttttaatc tcttatcatt taatgggagt ttatcaaact gagataaacc ttttcctcca
   126901 tctaaataaa atccttcact ttgattggct gaaattgccc ctccccataa aaaatctttc
   126961 cctaatttca tgcatctacc tctttcacaa tataaagttt tataatttct atctcatatt
   127021 tagttataag atttttttca tactatacct tttaaataat tcaatttaaa aatccaatta
   127081 tattttttac tcactataat taatattgtc tattttcaat actttcatat taaatatcaa
   127141 tattagatat ctatgtatat aattatatat atagcaaata catttttata tcctcattat
   127201 tgttatctta ttctcatata ttgctgtata tttatatata aaaggattta tttcgaaata
   127261 gaggttgtat tgagaactaa aatttagact ttataaatga atggaatttt cattgttgaa
   127321 atactgaaaa tatatttgat ttcatataaa agaaagtgcc tcatgaacta agttcatttt
   127381 gagacactcc cttttttttc caagctatta tatctttttt taaattctaa tggagagact
   127441 ccaatttttt ctttaaatac tctgtaaaaa gtttttgtac ttccaaaacc aacttcttca
   127501 accaaatcta caatggatat attgttatta agtaatatcc actttgcttt ttctaatcta
   127561 tattcattta aaaaagttat aaatgttttt cctgtatttt ttttgaaaaa tttagtaaaa
   127621 taatatacac taaatcctac ttcataagac acatcttcta attttatagg ttgtttataa
   127681 tttttttcta catatttgaa tatattgttc aatctctcta atatctcttt agagtttatt
   127741 tcatattcta ctgtattttc ttcaatttct ctaggaatct gtctatataa tataggaatt
   127801 aaattataca ttttaccttt tattgcataa ctatatcctt ctattttgta taggtcttca
   127861 ttgtagatat ctatcaatat atcgaaaact gactttctta tttttgctga ccaatctcta
   127921 ctacattttt ttatactact gaataaatta attaagcttt ctttttcctc atttaataaa
   127981 attaaatcat taaaaaaaga tatatcaaat tgaaatacta atctttcact tcccggtgat
   128041 ggcagtatat aatgaatatc tcctcctttt attatcacta tctcgccttt ttttaattgc
   128101 ataggcttgt catttataaa tagatttact acaccttctg ttataagtaa tatttctatt
   128161 tccttatgcc agtgtggtgt tgttaatact tctccttcat ttgatagact acgaaaagga
   128221 aattcatgta ttaatttagg catttctaaa tatattgcca tatatatcct ctcctctcta
   128281 gtgtctaaaa attaatttat actatttaaa agaatacaca ttttatttta aaaacctcaa
   128341 atttataatt tatacaatac ctacacttca taaaatatat ctaatatttc tttttttgta
   128401 tttgcattta aaagtctttc acctatattt ttaccaccta aaatgtaagc tattttagat
   128461 attaatatca tatcttcatt gcctccagat tgtggcatag caaacataat tattaccttt
   128521 acaggtaagc catctatact tggccaatct acaccttttt tagatttacc tattactatg
   128581 gatgccttct ttacatattt tgtttttcca tgtggcatgg ccatttgata acctatgcta
   128641 gtagagccta ttttttctct atgttttaat gcctttataa acttactttt taaagttata
   128701 cttcctgtat cgtataattt atctactaat tcctgtatta tgccttcttt tgtaattgat
   128761 ttcaaatcta aaataatgct ttcttctgtt aatatttctc taatttccat ttgttttctc
   128821 caatagatta ttcttaaata atattttaat atcctctttg ctcttgcaat ctattatata
   128881 gtttatatag ttcttttgcc tatataaatt tgatatttcc attaaaatat tctttaaaaa
   128941 actttgatta ctaatttcta aataaatatc aatcgttcta gtaacattat tgatattcat
   129001 tatcccttta tttaatttac tttgcttatg tatatatata tttaaatctt ttcctaatga
   129061 aatagattca tatttactcc ctaaattcac acctattaaa ctttccatct gagctacatc
   129121 aaaattaaaa tatatattat cttctaaata cattcctaat aagtttgtat ctaagttcat
   129181 acttttttta ggtttagaaa gtaaagcatt ttttatatta ttaatatcat tttccattaa
   129241 tagtactgat acaactatat gtaatttatc tttcaaatct aagtttacag tagatattac
   129301 aaagtctaca tcatctaaat ttcgcttact caactgatgc ataggaacta tatcaactat
   129361 agtccattca ggaaattctc tttttaatct ggctgcaagg agctgagatg ttccatatcc
   129421 tgtatgacaa actactatta ctcttttact actcatattt ctttctattg ccgcttgaaa
   129481 atatgtggca agatacgaaa cttcatctat actaatattc ttcagattat catactctgt
   129541 cataatattt atagtcatca tacataatcc cagcacatct gaaaatcgct cttgtatctc
   129601 tcctaacaac gggtttttta tttggatatc atatcttaat ctgtttaaca taggccttac
   129661 atgagatgct agacttttct gtagtaagtc atcatttttg agatttatat ttaaaaatcg
   129721 tgacatattc tctatcatta tagatgaaac tttttcactt tcacttaatc tatctgattc
   129781 atcattgtaa ctactattat tttcgctact aaatccagat gataccaaaa aaatatatat
   129841 gtacataatt tcatctttcg ttatttttac tctgtatcta ttttctattt tatctataag
   129901 tgtactgaca tttttatata ctaactcgtc caaattatct acagaaattt ctctttcttc
   129961 ctgagattct atttgattac cttcttcgat tctctttaaa catataagta tatgggtaat
   130021 taaatttata tagtagggct gatttatagt atatcctaaa ttgtattcta attctgtaat
   130081 tatagtttct acaaatataa tagaatcaat atcaaataaa tttatcaatg cactaaatgt
   130141 agcagaatct aatcttgtta actcagatat atcatgttgt tcataatttt catctaataa
   130201 atcatccatc attttggcaa tcgatttcct tatatccact tctttaccta taatcttagt
   130261 tccttctaaa gttttattaa gttttaaatt gtattctttt atccatgcct ctatatattt
   130321 aaaatcattt actatagaag ttttacttac ataatattta tctgctaact gatttattga
   130381 agttatatta tttgaattta gtaataaata ctttaaaatc tccattcttc tctgctctac
   130441 tgataaagat ttattttcct tcgattgtat atttaaacta ttaattaagt ctatattctt
   130501 ctttgctaaa tcttctatta atattccata gcctcttttt atatcaattt ttatattaaa
   130561 agtttctaaa tatttctcaa tcatcttcaa atctttttgt aaagttttat cagatacatt
   130621 taatttatct ttaaagtatt ttataggtct atatttattt tcgtttagta tcaatttaag
   130681 aaattcaatt tgcctcgaca caagcatact tagccctccc tacctatgtt tatatatttc
   130741 tatgtttata ttaagtacta ttttaacata tttactaact atgtaaaatt actaagatac
   130801 ttctgtatca taaaagaatt tatgatacac cttactaaat tgatataata gataatataa
   130861 tttcaaaata aaaattaagg atatttagaa gtagttaact gattctaaat atccttatta
   130921 ttattctata tacttgttgt tattccatta attattttta ctattattta taatttaagc
   130981 accaagtaat tcaacttcac tattttcact ccttatagta ggctttgtta cccctataat
   131041 caatgctgtt attactgtac caatcaatat acaagctata tatgcaactg gtgcactaca
   131101 tgcaaatggt attacaaata ttcccccatg tggtgccatt aaagttatat ctaaactcat
   131161 acatattgcc ccacctatac aagaaccaat tatatttgct ggaatcattc ttaatgggtc
   131221 tgcagctgca aacggtattg cgccttctgt tatgaaagat gcccccatta tagcagttgg
   131281 tagagatgac tttctttctt ctttagtaaa cttatttttg aatattaaca tagctaatga
   131341 tattgctaat ggaggtacca ttcctcctac cattaatgct gctgatggtt caaatatatt
   131401 acttgaaaac atagccaatc caaaagctga agctgtttta ttaataggtc ctcccatatc
   131461 tgatgccatc atccctgcta atattgcacc taataatact ttattagttc cagacatact
   131521 ttgtaaccaa gattgtaaag cttcatttaa ccatgatgct ggcgtattaa ctacaaagaa
   131581 cattatagct ccactcgtaa atgcaatcaa aagtggacat atcaatacag catttactcc
   131641 ttttaataca tctggtaaat tttctgttat cttcattact cctaaagcta tatatcctgt
   131701 tgcaaaacca gctaccatac cacctataaa acctgaacct ccaataactg ccataaaacc
   131761 agctatcata gcaggagcaa atccttgtct tcctgctatt gatttagcta taaaaccagc
   131821 aagtatcggt ataaataact taaatgctga atcgcctcca agttgtccaa agaatccagc
   131881 tatagggttg tatgtttcat gagttgggtc tgatgcaaat ataccaaaca taaaagctat
   131941 ggctatacca attccaccta tcactacaaa tggaatcatt tcagatacac cactcattaa
   132001 gtgcttataa acaccctttt tctctgaccc ttgaagcatt tcttttgaat cttctaaagt
   132061 ctttccacct ggtttaattt ctttagccat tccattttca gcgtttataa ttaaattttt
   132121 agctaatgaa acattcttag catctactac tttcttttgt agcactttct ttcctataaa
   132181 tctactcagt tgaacttctt tatcacaagc tattattacg tattcagctc tttttatatc
   132241 ttctttagtc aacttatttt ctacacctat agctccatga gtttctattt taacttcata
   132301 tccaagttct tgacctgctt cctctaatgc tttttttgcc ataaaagtat gagctatacc
   132361 tgttggacat cctgttatcc caactataag acctttattt tgattattac taaaatctcc
   132421 tgattctgca gctttattat taaatatact taatatttcg tatggagttt ctgcacttag
   132481 tagatgctta agaaaatttt cttccattag atatgtagct agtttagata taatcttaag
   132541 atgttcatct gaacctgatt ctggaactgc tatcataaaa aatgacctaa gtttttgacc
   132601 atcattatct gtaatctctc tatctgttct taaatacatc attacaggtt gatttactac
   132661 atttgactta gcatgaggta tagctattaa attacccata ttagttgatg attctttttc
   132721 gcgtttatta atatcagcta taaacttttc tttattattt atataatttc catggtacaa
   132781 atgatttgcc atataattaa ttacatcttt ttttgtttgt aattgtttat ctagtacaat
   132841 acaatcgctt cgtagtattt tacctatcat aaattctccc ctccttttaa tttgttacct
   132901 cataattaat ttttattttt ataaaagcac tttaacttat ctatagcatt gaatagattc
   132961 attttaaact ctataacttc tcttgcagat tttatacatg gtggatatat agtatctggc
   133021 tcatataagc ttgggttttg ttctaatagt tctctgcact tcttataata tgccgactta
   133081 acatcactcg aaatatttat tttacaaact cctagtttaa ctgcttgtgc tatttcttca
   133141 tctggatttg atgaaccacc atgaagaact aaaggtatat ctactacttc tcttatttct
   133201 tttaataaat ctagtttaag ctctggttta aaaccttttg gatatatacc atgtgctgta
   133261 ccaattgcaa tagctagtgt ttctactcct gttttttcaa caaaatcctt agctaatttt
   133321 gaatctgtat atattatttc atcagtacca ccttcacttg atgttcctgt tgttcctatt
   133381 gttccaagtt ctccttctac ggacacattt aatccttttg ctatttctac tatttcccta
   133441 gatatagcta cattctcttc atatggtaaa tgtgagccat caatcattac tgatgtaaat
   133501 ccacatctta ttgcccttaa tatttgttct ttattttccc catgatctaa gtgtattacc
   133561 ataggtactt tactcttatt tgcttcttct atgcaactag ctacaaagct atctcctaga
   133621 aaacttagct catttgggtg tattgcaagt attactggtg catttttttc gttagcactc
   133681 tgtactactg ctttaagtat ttgcccactt cctatattaa atgctggtac agcaaattga
   133741 ttttcttgtg ccacttttag catttccttc atatttatta acatattgta cctcccgata
   133801 ttttaaattt taggttgaat ttgcttcttg ttaattttgt tgttgtttat atttaaataa
   133861 tatcagggta atgaagatca gaatataaca atcgttttcc ttagtgaaaa ggaactttta
   133921 ttatacactc taagttcctt ttcactaaat aatttttaga agtctgcatc atagttaatt
   133981 atatttctca tatacatctt ctctttttaa gtgttttatg ctttagcctt cttaactctt
   134041 tccaataaac aattagatag tttagctata aatttaaaga tattcgtatc gtagttagca
   134101 ctatattgga atcaaaaaaa ttaaaaagat actagatagc agataccaat tattaataca
   134161 ccataagtca tagatacttc tatcttaaat tcttgcatat tagttacaat agaaattaaa
   134221 taggactatt ataaaaatcg taaggtaaga ctgacattta ttctccaact atacaaatga
   134281 atactaatat agctccttct aattaaatca tactatataa atcatatata tcccaatttt
   134341 taaccttacc tctttatatt tactttttaa taaaaaaaga tattgactaa atagtcaata
   134401 tcttttagtg gatttaaata cccacgattt taatctaatt tttaatggtt tccttaacat
   134461 aatcttataa atattaaaat ttatctacct ataaaatttc tccattaaat tttattacat
   134521 ttttatacca atcaaaagat ttctttttat atcttttaag agtcccactt ccatcattat
   134581 ttttatctac atatataaat ccatatcttt ttttcatctc tcctgttgta aatgatactg
   134641 gatctataca tccccaaaca gtatacccta taacatcaac accatcaatt tcaatggcat
   134701 ttttcatttc ccttatatga ctagccaaat actctattct ataatcatca tctacagtat
   134761 tatctgattt taatacatct tctgctccaa atccattttc aacaatgaat ataggaagtt
   134821 catacttatc atatagttga ttaaggtaaa accttagacc tacagggtct atattccatc
   134881 cccaatcaga aatttttaag tatggatttt caactacatc agcctttcct gccgagtcat
   134941 tacctgttgt tttcatagtt ttatcagctg ttactacagt agttaaataa taactacatc
   135001 caataaaatc aactattcct tcttttagga tttttttatc ttcctctgta atatctaaat
   135061 tataattttt tctttcccat aattttagtg tatgtcttgg ataatgacct cttacatgta
   135121 catcagtaaa cataagttgt tctctattag attctacaaa tttaatcata tcctctggat
   135181 tgcaagaata aggataactt ggcatagctg ccaccataca tccaatttta aactcagggt
   135241 ttatttcatg acctatcttc acaacctttg cacttgctac aaattcataa tgaactgctt
   135301 gatacataac ttcttctttg tcttcctgct cttcatagat tattcccgag ttagtaaatg
   135361 cataaatagg atttgtagtt atactttgat tatttatttc attgaaagtc atccagtact
   135421 taactttatc cttgtatcta ttcattacca cttcacagaa gtttacaaag aaatctatta
   135481 attttctatt tctccatcca ccatagtttt ttactaaatg ataaggcatt tcaaaatgac
   135541 ttaaagttat tactggctct atattgtatt ttaataattc atcaaataaa tcatcataaa
   135601 atttcaatcc atcttcattt ggagttgatt catcgccctt tggaaaaatt ctagtccaag
   135661 caatacttgt cctaaaacac ttatatccca tttcagcaaa taatctaata tcttctttat
   135721 aattttcata aaaattaatg gcttcatgat taggataatt taaaccctca attactccat
   135781 ctgttatctt tcttgaaata gtatgagtac ctgcagtcat cacatctgca atacttattc
   135841 cttttccacc tttattatat ccaccttcaa cttgatgggc agcaactgcc cctccccata
   135901 aaaattcttg attagatttc atagaaatat ctcctccagt ataattaata aacaactttt
   135961 attacaggcg ttccaatttt aatatgttta ttttctttaa ctattacatc tgctaaatca
   136021 tttgtgttag ttagtattat tggtgtaaca atactatacc cttcaccttc tattttatcc
   136081 ttatcgaaag aaacaatcct atctcctttt ttaactttat ctcctgcatt catatgagta
   136141 gtataatact ttccatctaa catagtagtg ttaagaccta cgtgtattaa tatttctaca
   136201 cctttatcag aggtaattcc tattgcatgc tttgtaggaa aaatagttgt tattgttcca
   136261 tttacaggag acacaacttc tccctttgaa ggtattattg ccatgccatt acctatagtt
   136321 cctgatgcaa acacttcatc ttctacttca gataactcta tttgtaagcc ttctattgga
   136381 gataatatct cctctatacc atcatcttca tcaaaagcaa cttcttcatt accttcttca
   136441 cattcttctt tactcaatcc aaagaaatat gacaatactg ctcctagcac taatgctgtt
   136501 cctattccta atatatatcc taggaatggt gtataaactg gcattgagaa tatattatga
   136561 aatacatatg cattacaagt aacaccaaat acaccacata taccaccacc aatacctcct
   136621 gcaatagcca atactggaat cattcttctg tatcttaata taattccata aattataggt
   136681 tctgttactc ctgctaaaat tcctgtaact gctgttgaac ctgctaatac ctttaattct
   136741 gaattttttt tagattttct aaccttcata aataaaccta ttgcaatacc tatttgagca
   136801 aatactgctg caacagctgc accttctatt gggtctccac ccattgtagc caagttttgc
   136861 aaagtaattg gtgtgaagcc ccagtgtagt cctaatactg ctaagaatgg catagctata
   136921 cctattgcag ctcctgcaat tactctgtta aattcaaata atcccataaa aatattagat
   136981 attatatcac caacattagt tgctattggt ccaaataaaa tcacagttaa aggaaccatg
   137041 actaataatg atatcatcgg tactaagaat aattgtaaat cctttaatat aatcttcttt
   137101 aaaagcttat caacatagta ataaataaat atagcaacaa atatagggaa tacaccaaaa
   137161 gaataatcaa ttgctgttac tggcatccca aaaaagttag caccaccttt tgctccaatt
   137221 aaagctgtaa aattaggatc taacaaagct gctcctatca ctccaccaac atatggattt
   137281 gcactaagtt ttatagctaa ggtaatacct aagaatattg gtaaaaagta aaatacagca
   137341 tttccagctg ctgataatat agcataagtg ctacttgtat cagacatcca tttcatagta
   137401 gttagtattg taagcagtgc cttaatcatt cctgctccag ctagtgcagg tattaatgga
   137461 gaaaatgcac ctgaaattga ttcaaatatt ttagaaacta ttgaaacttt ttctccctgt
   137521 tcttcagtat tttgtccttg taaaactgct ttttttataa tagtttcaaa gtaatcagtt
   137581 actttatttc caattaccac ttggtattgt cctccactat ttactacaga tagtacatcc
   137641 ttcattttta ataactcttc tttgttagtt tttgaagtat ctttcagctt aaaacgtagc
   137701 ctagttgcac aatgaactaa actaataata ttttcttttc ctccgacata ttttataatt
   137761 tcttcagact ctctttcaaa tttcatacaa tctatcctcc attttcataa ttttatgtat
   137821 aaaaatagac ctaaatcaat acaattagat attaaaaact aattatacca acttaggtca
   137881 tgcctgccaa acagtaacaa tcctacacta tttactttat atttgataat ctatttaaat
   137941 gcaatgttaa gtacaacaat tcattttcgc taattttcca tttatacgtc ttttcaagaa
   138001 agttttttac tttttttgca caattgtaat cacttttata tttcattttt attatatcta
   138061 acaatgatgt gtctacattt gtattctcac cacttaattg cctttttata aaacatctta
   138121 aatgacttat aaaccttgtg tagtcaaagg aatattcatt tatttcgatt ccatagtgat
   138181 actttatgat atctaataca ttatcaatta tcttagttaa caataaagtt tcttgcatat
   138241 cttttgtttc taactgagca tttgcaaagt gtatcgtaat aaatgctatt tcagaatcag
   138301 gaatattttt atctaatttt tttcttaaat attctacagc tttcttacta aaattatatt
   138361 cattaggata tatttgtctt atttcccatt gaagcgggct ttgaaatact aatccttcat
   138421 ccattctttt taacatataa cttatatgtt ctgataatgt gattaatata ctatcattaa
   138481 gattatcacc caattcttct cttcccattt gaataatttc acttacaagc tcaacatctt
   138541 ttaaaggtat cctatctagt agtgaatgta attcacttaa tgattcctca ttattgaata
   138601 taaatttctt ttcaatttta tctatttcaa ctaaatcttt agtaaattta ttaaatccaa
   138661 ttccttttcc cattaatatt acttcttgtc ctgaattatc tctggcaaat acaacattat
   138721 tattaaatac tttctttata accataaact acctccactg taaacaaaaa gagacctaaa
   138781 aaagactaat aatactttaa gcatttacta gaatttttag gtcacgcctg attacatcta
   138841 tctcagtaac gatccgttta tttatctgta tgataacttt attttcatta taaaataata
   138901 actgtactta gtcaatatat aatttcatgt atatctctac tagattattg tcataagtat
   138961 tcactcattc tacttttttt atatgattta tataatctaa acatgctaaa ataagttttg
   139021 aagttatttc ataacaaaat taaggtcata tattttttat tatatatact cgattattta
   139081 tccatatata aggaaatcct ttattttgta actacaataa ctgcctaagt tttgattttc
   139141 ttttatcttt tacttctcct tattaaaaaa taattctttt actgttgtat ctaaggctct
   139201 aaacagttta atcatcaaat cattgtttgg attattaacc ttacctaact tactatatag
   139261 ctttttaact gtatatcttt aatagacctt ttggtatgct aatccatttt ataacactaa
   139321 ttcttgaatc aagcatatat tacccttaca aagcatatct tataccctct gtagagttat
   139381 gatagttgat gaacctgaag aaattaccct tacaagacat atctgataca acctcactta
   139441 ttagtttata tgtttcagaa gtatataaat aacacatact tacttccaaa ttcttaactg
   139501 ttttcttaga aaattctaat atgatttcct tacatagcga ttataacagt gtcttattag
   139561 atttcataaa attatacatc ctctttacat ttaaacttta aatttttatc tattgtttat
   139621 taattaactg tatattcact attttttatc atttataata ctcttcttat tatcttaaaa
   139681 caattataat ctaaccttaa tcacatatta tataaatatt tttaataaac ttaataatta
   139741 aataactata tttcattatc catcttataa atgtttataa actttccact attcttcttc
   139801 aataaattca aacaaatttt ctactgtagt atcgaatact ttagcgatat ccatagctaa
   139861 cttcaaagaa ggattatatt tcccattttc taagtgtaca atagtctcac gtcttacccc
   139921 aaccaattta gcaagttcat tttgcgtcat aaggagtttt tctctgtatt ctttaatttt
   139981 tgttttcatc aatattgcca cttagttaca tatccccctt ttatcaaagt atatatacaa
   140041 cactaattta gataaagact gtataaataa aactgcaatg aggattatac ctgtatctag
   140101 gtttgatata tttatattac ttgtataagg tgttgcaaag aaaatagata ttattactat
   140161 agaatatatc attattttta ttgcaatctt attaaccttt gatagtataa attcagatga
   140221 ttcatcaact aaatcattat attttttatt ataaattgaa taacatccaa aaactattaa
   140281 ccaactcaac cctggaagag aagcagtgct tggaccttta aaaatatatt tagtagctaa
   140341 tacagctata aaaaaataag ttattacaag taattccaag atgagctttg atcttacacc
   140401 agtttctgat ttagacatat aaaatcactc cttaaaagtt atatattctc tatatttttt
   140461 aatttttgtt ttcataaata ttattactta gttacatatt ccctttttat caaaatatat
   140521 atacaaaatt aattttgaca aagattgtat gaataaaact gctaagagca ttatacctat
   140581 atccaaattt gatatgtcca aagctcttgt atgattaact gaaaagagaa cagctattac
   140641 aaacatagag tataacatta tttttgatgc aatctgatca acctttaaca acataagttt
   140701 tgatgcctca tcaactaaat cattatattt tttattatac atgagattgc acacccaaaa
   140761 agctaataaa attataaaag taaaccaaat accttctcct ttaaaaataa acttttgtaa
   140821 taacaagtct ataaaaaaat aggatagcat cattaactct gaaacaagat ttgccttaac
   140881 accaagctct gatttagaca tataaaatca ccccttaaaa gttatatatt tataacacct
   140941 tatgttataa atatataact tttccaaata aattgcaata gaatattgaa aatattcaat
   141001 aatatcttca taaatatatt ttataaaaga ttttataata aacattacaa tacagtacta
   141061 aattagttta tttcatctat catctcaatc atatcattta ctaactatta ttctaatgag
   141121 catataacat tgaaatttat aattatacaa cctaaatgcc tcccaatgta ccctcaaaaa
   141181 catcatttcc tgtaactgaa gtataattgg tatattttat cactgaaata aaaaaacact
   141241 agtcaataaa gactagtatt ttcaagtatt ttatcattct ttttttaatt attggagacg
   141301 aatcatcaga ataatgccca taatttatct atatgcaaat tctaaattta aaataaagtt
   141361 tcagatgaat ttactattct atgctatgat acaacagaaa aaactgaaat ctatgagtgg
   141421 tagcttcata taatcctagt tcctacagta atcaaaactg aggtttattc tatatgtaac
   141481 taatgataaa ttttattata ggagttatta gctctatttg tgttactata agtagtaatt
   141541 atatatatga taatttttta acacttttct gttggctata tagaaagtgg tttagaattt
   141601 aaatttaaat aaaatttaaa ttcaccaaaa agaaatctat attagaaacc actactctat
   141661 tccaccagat tgtaattttt tgttctttat atcacaatgc tataccatat taaattgaaa
   141721 aataaatttt aaatttattt gttacaattc attaattcaa aaaaatattt tgagtcatga
   141781 gttttgtaaa gatttagcca tgaatttaat gcttctgatt caaaaacatc taaaagtttc
   141841 aatacttgaa acgtaaattc caacggagcg attcctgaag ccgtaactaa attttcatca
   141901 ctaactacag attccgtttt ataatatttt tctccagagt aatttggtat taccatttta
   141961 agaaactcta agtcattact tgtatgattt ctaacatcta atagcccttt tttagctagg
   142021 cctaatgtag ctccacatat ggctgcaact atagaatctt gtattaaagc ttctttaact
   142081 ttttctaata ttatctcatg attcatggat aaccaagtat ttccccctgg caaaattaat
   142141 aaatccttac tttccatatt aaactcatca actgtaattt caggtaaaac ttttaaccca
   142201 cccattgtta taataggatt tttatcaatt cctacagtaa ttacttttag tggctttaat
   142261 ccttttttaa aatatcttcc tgtatttaat tcagcagtta aatatgccac ttcccaatct
   142321 gacatagtat caaatacata catatatact tttctatttt tcatcttttt tactccttat
   142381 gtaattaatt tatatatcta attataaaat aacttaactg acatattcag tcagttaagc
   142441 tattaaattt gataataatc aattaattct agtaaatctt caattatttt cttcctaagg
   142501 cttagtggtt caattacttg tattgtcttt ctataaggta aaagtatatt tggtatgtac
   142561 ttgcatatta cttcctcttc gagcataaat attattttat taggtgttct ttctttcaag
   142621 tagtgcccta aaaaccaatg ttggcttaaa ctatctaaag aatctatact cccttctaca
   142681 actaattgaa ttatattttg ttcatctttt atttcaggta taattccttt tataaaaaat
   142741 tcacttgctg aaaattctgt cggtctgtta aatgtatttg atgtttctat gatttcacta
   142801 atcctatcca ctctgaaact acgtacctca tttctcatat tacaaaatgc aactacgtac
   142861 caattattat tccagtgaac aattccatat ggatttataa ttctatattt tgattttgct
   142921 ttacgacctg catgatagtt gatttctaca gatagctcct ttaatatact attctccaat
   142981 tttttaagta ctgattctat agacactttc ccaattggtt ttattacttc aagggctctt
   143041 aagtggtttg ttaaaatatc ttcttggttt tgatttgaat acatttttaa ctttaatatc
   143101 gatttatcta atgcttcgtc taagaagtat ccttcttttt tagcaaatag agctgcatga
   143161 agtagagatt tttgctcttc tatatcaaaa aaaagaggtg ctttagtaaa attatttaaa
   143221 aggctatacc caccattatg acctgtatct gaaattatag gtactccact cgcagaaagt
   143281 acatcaatat atcggtacac tgttcttata tttatttcta atttttcagc tatctgtttt
   143341 gctgtaattt ttttgtcaga gtttaacatc cacagtatag acatcatatt gtcaatttta
   143401 gccatgtagt ttcacctctt tctatttgtt atataaagtt attatataac tttttgattg
   143461 attttttaca aaaaaacatt agtaaactgc taataattta tactaagatt tatttcaaat
   143521 atttatagat agtctttttc tatagttaag ttatccatta aattacatag tagtatatcc
   143581 attttagttg atatattttc agctacctcc cttgaatatt attacaataa gtctaaatct
   143641 tctttcttcc ataaatatat tatgaggaaa gactatcatc tttaatggtt taatagtata
   143701 aaagatatta atttaaaaaa atctattttt atatcagatg ggtattgttt tttataaaac
   143761 tgaaaaaatc acctaataga atctatgatt ttttaacata acctccataa attagtacca
   143821 attatcactt caaattcaaa tttttatctg aagaatatac aatcatattt ccatactcaa
   143881 cataatattg agtgtttggc ttgtatttac aaattattat tctaacaaaa acaactatac
   143941 atagattaaa atcaccttct aaacataatt attaaaattt aattaatgat atcaattcca
   144001 ttaatctctt ttaataaaaa attctctttt ctatatgaaa attatttcaa aacttttaag
   144061 ttaaatacaa aaatgactat aaatagattt ttatttacac aaatctattt atagtcacta
   144121 tagtttatcc aataaaataa taaaataaat atgtacacaa taaatgttat ttacgtctta
   144181 agtatttatt tgttagattt ttaattaaaa aaaacaagct aatgaacatt aatgctaaat
   144241 ctagcattat attactttga tctcctgttt gtacattttg acctacatta ctatctaaac
   144301 tttcatctgt atctatattt atatctggtt ttccattccc atctgtatct atatttatat
   144361 ccggctttcc atccccatct gtatctatat ttatatccgg ttttccatct ccatctatat
   144421 ctatatttat atctggtttt ccatctccat ctatatctat atttatatct ggttttccat
   144481 ctccatctat atctatattt atatctggtt ttccatctcc atctgtatct atatttatat
   144541 ctggttttcc atctccatct atatctatat ttatattcgg ttttccatcc ccatctgtat
   144601 ctatatctgg tttgctattt cctccattat ctgggtctgg tttactattt ccaccgttat
   144661 ctggatccgg tttgctattt cctccattat ctggatctgg tttaccattg ccaccgttat
   144721 ctggatctgg tttgctattt cctccgttat ctggatccgg tttgctattt cctccattac
   144781 ctgaatccgg tttgctattt cctccattac ctgaatctgg tttgctattt cctccattac
   144841 ctgaatccgg tttgctattt cctccattac ctgaatctgg tttgctattt cctccattat
   144901 ctggatccgg cttgctattt cctccattat ctggatctgg cttatttaga tttggattac
   144961 ctgtatcata acctcctgag ccagtatcta catttttatc aggcttccaa taatcatcta
   145021 ctccatcatt atctgtatct attgctggac gattataatc tgtatcaggg tttccatctc
   145081 cattggtatc tatattttta tcaggtttcc aaataccatc tccatttcca ccttctgtac
   145141 taggcttcca ttcacctgta ttatcagtat ctatgttaat atctggcttc caatcaccac
   145201 tcgtatctat attgatatct ggccttccat caccatcagt gtctatattt atatccggta
   145261 ttccatcccc atctacatct acatttgtca tatctaactt taacttcttt tcatcctcac
   145321 taccttcaat gtaatttata tcatctccaa tatattttac agtaatagtt tgttcagtag
   145381 taaaaatatg atgattgcta tctaaaggtc tccattcaat agatgcagta ctataattat
   145441 gactctcact acccttatta tgagttttta attctactgg atctttatat tcctttccat
   145501 taatatagaa ttgtacgtaa cctcttggag aggcacaatc tggtccttct ttcttatctg
   145561 gtgaaatagt aacatttaat gtaaacgtat ctgttggttt aaaaacatca gttttagctt
   145621 tagactcttc tacaactaat gaagtttctg tatcagcagg aataatttct gccttataat
   145681 atgctctatg cccccaataa gcttcattaa aaggatatac atttgaaaac tgtgttgatg
   145741 taatgcttaa ttgatatttt ccagcatcaa cttgctttgt aaggtcatca cttcctaaat
   145801 ttgtatctgg ttccattgtt tcctcattta aacgttgaga actaatagtc ataaattttt
   145861 tatctgtgag tttttcacct ttaggagtaa ttattggaat acccccatct tttaatatcc
   145921 aatctaaaaa taatttttct acatcttctt caaatggtaa tccatcatat cttttaataa
   145981 tcaaatatgg tggagtatca tcaatagtaa attcttcata ttgctttaaa aattgagaac
   146041 ctgaacctat atctttaaca taaaaatcat ctggttttat atttcctcca gtaattttat
   146101 ctataacaac taaattaaca gatacttcag atcccttaaa ttgatcagaa aaccataagt
   146161 aaagaacccc ttgatcatct gtataactag gtgctccata aggataaatt tttccatcaa
   146221 ttagcatttt aaaatcatca actaatgtag tactaacttt atcttgtcct aaatacttta
   146281 aagatatttt attcataaaa acttttgttt tcttttctaa atcaccgtat gcaactgctc
   146341 cacctaaact tttgaatgtt gttgctttta atgaaccacc tgttactata acatctcctg
   146401 ttgtagcact taggtcaaaa atccaatctc ccactcctgt catataaggt aaaatcgttc
   146461 ctccagttac aagtatacga tatcctctat ttgttcctgg attaacacat ccatccccaa
   146521 atgcactacc atgtgcacct ccatatgaat aggaaagtcc accattaatt gtaatatttc
   146581 cactataacc agaacctgtg ctaggatcta ctactgaggg acttaatgca ttctttgttg
   146641 ttgtatagta tcctccacct attccagctc catggtatgc acctttagca gttatagtac
   146701 caccattgat tgtgatatct ccaccactac cattaccgcc tccacctatt ccagctccat
   146761 atcctgtatt acgactcgat tcttgaccta tagcagttat agcaccacca ttaataataa
   146821 tatttcctcc actttcagta tttccaccgc caatacctgc agaataataa tttccaacaa
   146881 cttttaaact tcctctatta ttactatcta gtagtgtaag gcgatctcct ttttttaact
   146941 tgtctccatt ttctagagtt acatctctag gaatttttcc accctcagga actataaact
   147001 ctccttcaat atctctattt acaacagaat catcaatagt tagagtagta ccttttcctg
   147061 ctctcatacc tggataatat acatcacctg actctagctt gttttcacta ccatcttgta
   147121 atataacatg tgcatatgca ccaccatctt tatctggtgt taaagtaaat gggctattag
   147181 atgcagtttt aatatttact ccatcaaata ttaattcagc ttttacatct ttaggtataa
   147241 caatcctatg tgttgttgga gtatttggat tattattttg aattgtcatt tgttttccac
   147301 taagaatagt taaaacgcca cttgcaaatt taaaatcgac atttatttct ccaccactaa
   147361 ttttaaagtc tcctacagat gtaggatctt ctgcagtggc ttcttccaca actaatgcat
   147421 tcatatcttc ttctgcattt cctgcattag aatcttcttc tgcatttcct gcattagcat
   147481 cttcttctgc atttcctgca ttagcatctc cttctgcatt tcctgcatta gaatcttct
//
LOCUS       DAMJVI010000002       129213 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.002, whole genome shotgun sequence.
ACCESSION   DAMJVI010000002 DAMJVI010000000
VERSION     DAMJVI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 129213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 129213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..129213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.002"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            51..386
                     /locus_tag="PC545_000113"
     CDS             51..386
                     /locus_tag="PC545_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2454911.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQEDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNVVESTYFLSESGLRLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            523..1800
                     /locus_tag="PC545_000114"
     CDS             523..1800
                     /locus_tag="PC545_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDF2454912.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMKAITAFILGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIRSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNTSQNSKIESKQEENKRENSTDKEENSDGNDSQKDSNNDNNDKG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPYIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            1921..3309
                     /locus_tag="PC545_000115"
     CDS             1921..3309
                     /locus_tag="PC545_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2454913.1"
                     /translation="MKKTFDAIIIGFGKGGKTLAGDLANRGLKVALIEKSNKMYGGTC
                     VNVACIPTKSLENSANSVKTKNINSWDEVQAEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENEIYELVADNIFINTGSRPFIPNIKGIENKNIVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGAEVTILNSNNGILPNEDVEDSEEI
                     IKLLAKRNVKIVNNANIKEIKEVSELAIVEYEVDGKSKELTSNMILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIVINQLFGD
                     KTRTTNDRKNIPNSIFISPAFSRVGLNVKQAKEKGYEVLVAKMPVEAIPRAKQIGKAD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPNMIKEV"
     gene            3680..4393
                     /locus_tag="PC545_000116"
     CDS             3680..4393
                     /locus_tag="PC545_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017208807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HDF2454914.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELTRLIKAKEEIEWLLNRDYKIDP
                     VVTFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKEDTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIIDKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKECNIINLNK"
     gene            4511..5380
                     /locus_tag="PC545_000117"
     CDS             4511..5380
                     /locus_tag="PC545_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2454915.1"
                     /translation="MRGWICVKLTKLNIKKYRASIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDDKIGYVFNYFLSIDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPETPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(5535..6680)
                     /locus_tag="PC545_000118"
     CDS             complement(5535..6680)
                     /locus_tag="PC545_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2454916.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKDSIKSLGTKKAFIVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYDEVKQNPTVTNVNKGLKILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIIAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDTNSLATMTVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSAPAKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENSMKDACAGANPVFFDKDKIIELFMKIS"
     gene            complement(7014..7790)
                     /locus_tag="PC545_000119"
     CDS             complement(7014..7790)
                     /locus_tag="PC545_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2454917.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADVINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPD
                     GLKGFTEKSITDFDVLYDQLLEVQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            8372..9346
                     /locus_tag="PC545_000120"
     CDS             8372..9346
                     /locus_tag="PC545_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2454918.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            9444..9749
                     /locus_tag="PC545_000121"
     CDS             9444..9749
                     /locus_tag="PC545_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2454919.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTVKTITALDDI
                     TIYHKLAIKDMAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            9905..11065
                     /locus_tag="PC545_000122"
     CDS             9905..11065
                     /locus_tag="PC545_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2454920.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQIDKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            11115..12017
                     /locus_tag="PC545_000123"
     CDS             11115..12017
                     /locus_tag="PC545_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2454921.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNVSSEDIIKLKTIINESTFYNYDVFKRERCTVHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDDSFEEPQKTVYFTKRVTKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSAVPFPIK
                     LKVCSRVNGEERQSSNTGLFISDVSDIVSELSKGMTLEAGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            12097..12990
                     /locus_tag="PC545_000124"
     CDS             12097..12990
                     /locus_tag="PC545_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HDF2454922.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDKEGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVNKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKIEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMEKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     regulatory      13043..13224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            13452..14582
                     /locus_tag="PC545_000125"
     CDS             13452..14582
                     /locus_tag="PC545_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2454923.1"
                     /translation="MKFKSNVLKQFFLIAMVIFMVGGLIGCSKSDKENKQASTGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDMA
                     TSELNKTVQKVEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     gene            14654..15679
                     /locus_tag="PC545_000126"
     CDS             14654..15679
                     /locus_tag="PC545_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2454924.1"
                     /translation="MDFINKMRIRNTTIFIVLIISMILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGACLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVKNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILIIVGILGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIRFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQIS
                     KMIFKTSDNRVLIPVVIFLGSIVTALCDLISRVILSPVELPISTITSLLGAPIVVMLL
                     VKRKDRA"
     gene            15682..16878
                     /locus_tag="PC545_000127"
     CDS             15682..16878
                     /locus_tag="PC545_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2454925.1"
                     /translation="MLKTNNLSVGYNNKVVISNINVEVKNGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINCISRKALSKKMALVLTNRLLGDLMTVQDIVNIGRYP
                     YTGFFGSLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKKLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDEDIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFEPISDESFNLAIRNLNDSKIIIDT
                     GFSVGETNKRNIDIIKEALKLDKKVYSFRNRDESKKYYDSLDSKIEHIDKVSQIINSA
                     DINNLL"
     gene            complement(17006..17731)
                     /locus_tag="PC545_000128"
     CDS             complement(17006..17731)
                     /locus_tag="PC545_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2454926.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNAGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGASFRFCDLS
                     NSDIRGANLENSNLEHATLNGVISDESTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAEDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            18209..20308
                     /gene="nrdE"
                     /locus_tag="PC545_000129"
     CDS             18209..20308
                     /gene="nrdE"
                     /locus_tag="PC545_000129"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDF2454927.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDKEALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSIDVIKNIYDMIDKHKFEFQSYMSANKFYQNYALK
                     SNDGKEILETYNDKVLIVALTLGNGDKDLALNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMDEWYDKLVNDSDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDVGIIKMSNLCCEIFQYQTPSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIERNQT
                     FEGFDKSEYVKGRNSKVLSKYYEQSYLPKSEKVRALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAVAPTQSISYVQNATSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNMLYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            20320..21291
                     /gene="nrdF"
                     /locus_tag="PC545_000130"
     CDS             20320..21291
                     /gene="nrdF"
                     /locus_tag="PC545_000130"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDF2454928.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKVWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNLEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEMLEEKMYSILYSLMENEIEYTNVIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            21367..21993
                     /locus_tag="PC545_000131"
     CDS             21367..21993
                     /locus_tag="PC545_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2454929.1"
                     /translation="MKYEWRKRDKELYLPKKNPVIVEIPEQKFIMLKGSGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKGLDVLIKEDLIYT
                     IMIRQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYTHREIYISDARKVSPESLKTVLRFEVEREV"
     gene            22388..23017
                     /locus_tag="PC545_000132"
     CDS             22388..23017
                     /locus_tag="PC545_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454930.1"
                     /translation="MNKYLLINPVAERMYGEKFHLIKANLIKKGYIITECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESISLLKRNNLINNFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLVVTCPCTQLRDFASEKFKDKGNAMFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGFEDKYDIVEMFYCKDGCNNGDGL"
     gene            23018..23800
                     /locus_tag="PC545_000133"
     CDS             23018..23800
                     /locus_tag="PC545_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2454931.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGVPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVEAYE"
     gene            23793..24671
                     /locus_tag="PC545_000134"
     CDS             23793..24671
                     /locus_tag="PC545_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2454932.1"
                     /translation="MNNLVCDSGYIIRNLYKSYKVDNKEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLEGINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     AFHNQRKNTILNKVLRKFKHNKRIRSKIINDNFRSLETNDNLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLLMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKVKEFNIEDGYNRDLTSDYYI
                     KLKKDILMTMKSNKGI"
     gene            24704..25663
                     /locus_tag="PC545_000135"
     CDS             24704..25663
                     /locus_tag="PC545_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2454933.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSVDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYA
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            25977..26669
                     /locus_tag="PC545_000136"
     CDS             25977..26669
                     /locus_tag="PC545_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2454934.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSHIRRVYGEYSPKIDERIVELDGLKFYPERLELELNGENLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     VRGLGYKLNKNW"
     gene            26679..27683
                     /locus_tag="PC545_000137"
     CDS             26679..27683
                     /locus_tag="PC545_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2454935.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVMTIIYCNLMKFISFGESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGVHSLEESFLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHSEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIRKEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQVDKFTYVYTDV
                     KWMKFIIGQLITNGVKYSKDYSNYLTIKSEENNKYVILSIIDEGIGIVSKDLKRVFAP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSIYFNKGVNSLIKM
                     "
     gene            27931..28782
                     /locus_tag="PC545_000138"
     CDS             27931..28782
                     /locus_tag="PC545_000138"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2454936.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVIEFFKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSIMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAVYRIIMGVVLAVLA
                     FTNIISV"
     gene            28886..29632
                     /locus_tag="PC545_000139"
     CDS             28886..29632
                     /locus_tag="PC545_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2454937.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKGKNPLLLRGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDERLNEVSTILGIKDLLGKRTFEVSGGQAQRTAIARALINN
                     PSILLADEPTGNLDSKSSKVVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     SIYNEIYKGSSREQFYQEIMDVLTLLGGDN"
     gene            29632..31548
                     /locus_tag="PC545_000140"
     CDS             29632..31548
                     /locus_tag="PC545_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2454938.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASIVISSSLLFSFIMFTSHPDL
                     DVSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISKRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSIFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAITST
                     DQYTTYLRLVPVTSLTIVATYLFFSQFSVFFMKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVERQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMMFIENNLKKENVDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITETIG
                     VKPISFEDNECISLLSSSLPGNKNVKENIIVGNRGLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFNNESGMQPYLFFSKELMIGT
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLFPYVVAVIHSIFALTALKNSFDIEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIRLNLVNF"
     gene            31827..32744
                     /locus_tag="PC545_000141"
     CDS             31827..32744
                     /locus_tag="PC545_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2454939.1"
                     /translation="MMILIQSRGKMKCKELSEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKNEFNNIVEKIKAVLN
                     TGEKSDTYMDYFTVQAQRNCDYESEKNKCNEIIRAYTTKHKFWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKDYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVEPDRLRNDILLEIEKMKKIY"
     gene            32822..33559
                     /gene="cas6"
                     /locus_tag="PC545_000142"
     CDS             32822..33559
                     /gene="cas6"
                     /locus_tag="PC545_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2454940.1"
                     /translation="MKMKIEFSTGCIPISYNSLFMSIIKEAIRKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYIKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGNGLKRKLEFENIGLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNLIYNLGIGFRRAQGFGDVDILEWR"
     gene            33565..35415
                     /gene="cas8a1"
                     /locus_tag="PC545_000143"
     CDS             33565..35415
                     /gene="cas8a1"
                     /locus_tag="PC545_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HDF2454941.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKADKQAEIFIMEDYIEFDSL
                     FLENFHEYYFNYFMDEYDVSKRIKKNIDYSINFIKSKPDRIKDGIKKIKDSVKQQNDK
                     IKKFDEEKFNMIKEKLDSMSKIKSYDEFDSLEALVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDINEKKVSKSSINTIEKIMKEINKNFIKKNKSIEDIKKYMDSLEVCEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWNQAYVPSICDICKLILFCTPAGATYTR
                     KNYLINEENEFYLFVNMDTCINELFERNNSLKAQKSEYSDSKDENPFNQLIKSIVEEN
                     TLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLPNEKEEK"
     gene            35417..36331
                     /gene="cas7i"
                     /locus_tag="PC545_000144"
     CDS             35417..36331
                     /gene="cas7i"
                     /locus_tag="PC545_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2454942.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRDDGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREATVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGDIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVIQGTFLGKDLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            36333..37034
                     /gene="cas5b"
                     /locus_tag="PC545_000145"
     CDS             36333..37034
                     /gene="cas5b"
                     /locus_tag="PC545_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HDF2454943.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LEEIYDSLLNYDEHLSLGRKEDLVRINDIRFVEVNKFEVDEDIQLDDYENYKLSECNI
                     RMPIYIPKTSLQDTDIDGISYRLNNYYDNNAVEDKKRVWNKVDSYYVEDGNTISIGDI
                     LLDNEGDLLYFNNIN"
     gene            37045..39366
                     /locus_tag="PC545_000146"
     CDS             37045..39366
                     /locus_tag="PC545_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2454944.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGKNINSLDFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIKSRMDTKINEPKIVTYLENNVGHNYLSPAFIDY
                     SYIDRKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVIN
                     IGEQTENYLMKEFGSLREAQSFAKSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGRFMIMTATMPTIYIDELKKRGVMNSNLAELTCNTEKIRHCVSIVENSIDENLGKII
                     QSGMNKKVLVIVNTVKSAVEKYELIEEIVKPKGIDVNLNLLHSMYIQEDRAKLEKYIK
                     EFANSDRNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKTFINGKECVKMKEKYKVEMVKILYSKESLEG
                     TAFEKRFTSALNILDTITPYSLGSKDAQNILRNIEGYTVIPEDIYNKIEETLIKDYEE
                     LGQELINAYSNDDRQLINKIKSKRKKARREIIKKTVNLPIYKAKQNVIDIQVKGLEDL
                     KILLYKYDIYENKHTKQIFGKGVLISNEIDQFI"
     gene            39381..39932
                     /gene="cas4"
                     /locus_tag="PC545_000147"
     CDS             39381..39932
                     /gene="cas4"
                     /locus_tag="PC545_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDF2454945.1"
                     /translation="MWLKFTKEGDFVPIDLEVLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEILIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMQLSP
                     PKIEKKHYCTKCAYYEFCFVKEE"
     gene            39935..40915
                     /gene="cas1b"
                     /locus_tag="PC545_000148"
     CDS             39935..40915
                     /gene="cas1b"
                     /locus_tag="PC545_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HDF2454946.1"
                     /translation="MYKDYYIISNGDIKRKENTIYYYNLEGEKKILPVEQVDDIHIYG
                     EVDLNTKLINYISKYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDTIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTITFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            40918..41184
                     /gene="cas2"
                     /locus_tag="PC545_000149"
     CDS             40918..41184
                     /gene="cas2"
                     /locus_tag="PC545_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDF2454947.1"
                     /translation="MFVIVTYDIVEARSLNRIRRILRKYLTWTQNSVFEGNITEGKLH
                     KCISEIENIIDNSEDSIYVYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   41368..43029
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=41368..41396
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            43090..43518
                     /locus_tag="PC545_000150"
     CDS             43090..43518
                     /locus_tag="PC545_000150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2454948.1"
                     /translation="MIKKLNNKDINKIMEIWEKSTIKAHDFISKEYWQNNYNTVKNEY
                     IPISDTFVYDDGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATKKYKSLSL
                     AVYKDNKKAVVFYNKKGFNIVKEQVNEDSGFKEYIMEYSK"
     gene            complement(43973..44752)
                     /locus_tag="PC545_000151"
     CDS             complement(43973..44752)
                     /locus_tag="PC545_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454949.1"
                     /translation="MKVNENNINNIDEELDELSRKVYLESPDFWISISDKIIDKVFDE
                     MLLFFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYL
                     LSLNKGNNLDERPILDELNCNISSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDIT
                     YKFSSKEAEPIEMCSEVSSIRCCNCDELLEDITLDKLYNLCKIQNCNNCGSNLTKVGI
                     IAKKKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            45076..45621
                     /locus_tag="PC545_000152"
     CDS             45076..45621
                     /locus_tag="PC545_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDF2454950.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRRNL"
     gene            45653..46789
                     /locus_tag="PC545_000153"
     CDS             45653..46789
                     /locus_tag="PC545_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HDF2454951.1"
                     /translation="MKEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKDLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKVKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNTNNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIEKADLDFWVKESHKRAKEAREEKILEDIISPINNSTYDEGIRDKMSQRLLDRMP
                     SILGKETITNAANSCLINDGASFIIICSKKYLEHVKKKPKAKIINTCTIGTDATLSPT
                     SAIQAMDKLLEIESLNYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALLLERV"
     gene            46793..48184
                     /locus_tag="PC545_000154"
     CDS             46793..48184
                     /locus_tag="PC545_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HDF2454952.1"
                     /translation="MNYYELLRIKKERHKNKNFLIIDGEKYTYENILSYSEELGKQIS
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNFSIFNPKFNIYMYQYKNLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNGIFNISGESILFVNGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRIIKQYNVTNIYLIPTKLQLLVKHLKEPVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIEKPLSVGRPFPGIDIYIREGKIFVNT
                     EYAVYNATKPYSVNDIGYYDNDGYLIFEGRSDDILNIGGFKVSSTKIENEVKKIPQIE
                     DAIVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            48260..49135
                     /gene="dpsA"
                     /locus_tag="PC545_000155"
     CDS             48260..49135
                     /gene="dpsA"
                     /locus_tag="PC545_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HDF2454953.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDDCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAIAEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTANAFGYSSISLSQLDEKLLQFDYIFNTI
                     PECILTKERLEKVSQEVTIIDIASSPGGVDYSVVKEAGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            49138..49728
                     /locus_tag="PC545_000156"
     CDS             49138..49728
                     /locus_tag="PC545_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HDF2454954.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEEAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(49935..50135)
                     /locus_tag="PC545_000157"
     CDS             complement(49935..50135)
                     /locus_tag="PC545_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454955.1"
                     /translation="MEKLKTISVFSLIISVILTIGGIGIVTYYVDNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            50639..53293
                     /gene="adhE"
                     /locus_tag="PC545_000158"
                     /gene_synonym="adhC"
     CDS             50639..53293
                     /gene="adhE"
                     /locus_tag="PC545_000158"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2454956.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIREAQKI
                     FATYSQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDDSADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMNKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKYTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     IASFLGLKGNSDEEKFQSLLVAIEDLKLKVGIPKSIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            53569..55788
                     /locus_tag="PC545_000159"
     CDS             53569..55788
                     /locus_tag="PC545_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2454957.1"
                     /translation="MTGRNKSFTLITILFLTSLFLTMGYIYIEDTKHLLMSEAKIHIK
                     EVAVQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDEMENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYVDEVNKSIKKIAISVPLLNNDK
                     VVGVLYCTYNINTLMKLINISFYENNSISYVVKNDGTIILHPQGDSLSKNIYKLLEQD
                     NDIQEVNRLKKELQENKTGATVLNMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSKQIINRAVYAVLSIVLIFVAIIFYIIYIKKSNEKEILSLAYEDKVTYIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVYARLSSDYFAIFCDYKNGRNKIIRKLDNIRSNIESNLSIVFEISLCVGIYFVEEG
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVTGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFERVCND
                     LYEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIKMYNLSSDFIEIEITETVAINE
                     LNILKNVLNELRTYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLGGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            56042..56704
                     /locus_tag="PC545_000160"
     CDS             56042..56704
                     /locus_tag="PC545_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2454958.1"
                     /translation="MIDISIDDKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TINQSLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSQESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            57075..58484
                     /locus_tag="PC545_000161"
     CDS             57075..58484
                     /locus_tag="PC545_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDF2454959.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGVLFVAYIGMAIFFRNHFYF
                     GTTINGVKASGKTVEQVNDLLASSTNSYTLNLEERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMITLSYNKDLLEKRFNALSCFDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEAPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKDITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNKDDIGNTYVEISLSGQHMWFFKNGEMLVSTDVVTGDLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGSIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            58599..58874
                     /locus_tag="PC545_000162"
     CDS             58599..58874
                     /locus_tag="PC545_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif11-like leader peptide family natural product
                     precursor"
                     /protein_id="HDF2454960.1"
                     /translation="MNEKLTKILEEAKNDDEFRKELIKTREQRDVMDSFCKVVTSRGY
                     DMTVGELFSMGEEYTSNLLKSVNGGAAYPIEDWSDWYEDFFIMLSNI"
     gene            59504..59815
                     /locus_tag="PC545_000163"
     CDS             59504..59815
                     /locus_tag="PC545_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454961.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAEERKSKAIEAVIGKVV
                     R"
     gene            59817..61742
                     /locus_tag="PC545_000164"
     CDS             59817..61742
                     /locus_tag="PC545_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDF2454962.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEGQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPIENMETKYTKTILGTVP
                     ISANIDALRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHADSIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVIFFGMMLSDAAYGLIIAVVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGILGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            61745..62236
                     /locus_tag="PC545_000165"
     CDS             61745..62236
                     /locus_tag="PC545_000165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDF2454963.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            62288..62851
                     /locus_tag="PC545_000166"
     CDS             62288..62851
                     /locus_tag="PC545_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HDF2454964.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            62866..63843
                     /locus_tag="PC545_000167"
     CDS             62866..63843
                     /locus_tag="PC545_000167"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HDF2454965.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKIMSSAILEAFDVYNKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            63830..64153
                     /locus_tag="PC545_000168"
     CDS             63830..64153
                     /locus_tag="PC545_000168"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDF2454966.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            64270..66048
                     /locus_tag="PC545_000169"
     CDS             64270..66048
                     /locus_tag="PC545_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDF2454967.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTTKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            66045..67418
                     /locus_tag="PC545_000170"
     CDS             66045..67418
                     /locus_tag="PC545_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDF2454968.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            67424..68092
                     /locus_tag="PC545_000171"
     CDS             67424..68092
                     /locus_tag="PC545_000171"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDF2454969.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDTAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSDAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            68369..69418
                     /locus_tag="PC545_000172"
     CDS             68369..69418
                     /locus_tag="PC545_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2454970.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADIIWGLSAMNMIELKK
                     DGALEAYKPKNYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGEKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPIEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYAHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLNSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            69514..70503
                     /locus_tag="PC545_000173"
     CDS             69514..70503
                     /locus_tag="PC545_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2454971.1"
                     /translation="MSYLKINNVFKSYDQKKVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGTIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKVSKEKIDNKVKEVLETVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            70508..72226
                     /locus_tag="PC545_000174"
     CDS             70508..72226
                     /locus_tag="PC545_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2454972.1"
                     /translation="MNNNAISSKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASESLLNSLKNTVFVSVISTVISLTLALVYAYALTR
                     TKIKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGVHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLSSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGVVGIIAFGIIAVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLFTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFNFMDTIYIKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILVTNILVKVIYEFIKNRLE
                     KRTNFN"
     gene            complement(72308..73462)
                     /locus_tag="PC545_000175"
     CDS             complement(72308..73462)
                     /locus_tag="PC545_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2454973.1"
                     /translation="MKINKKFNRVDMQVSILTAIIVIISSSCVYFFNYTLTYKSMIYS
                     LSERSKHIYEYVERNIDKNTFTEINSKEDQSKKSYKSSKILLESVKNTTGVMYLYTAK
                     KTKDGSLVYVVDGLNSSRSDFRNAGDLIEKEIWGDLNKALDNHILLPKKIKSTSWGYI
                     FISYFPIHNDVGKVVGVIGIEFEAKHQYNTFKFISIVTPIIALLTCLISALIAVILFK
                     RISNPTYTDFANTDYLTGLKNRNAFEIDMNNLNNSNLKNLISIISIDLDGLKKINDKF
                     GHQTGDLYIKQASKIIQYVINKKYIVYRIGGDEYIIILKNLSHKEINNIINNINLKIV
                     EENSTNELKLSMSIGYAIFDEKVDVSLFDTYRRADSQMYDNKRKINKQYQ"
     gene            73961..75322
                     /locus_tag="PC545_000176"
     CDS             73961..75322
                     /locus_tag="PC545_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2454974.1"
                     /translation="MKDRLNLTEGRITEKLLKLSLPIMGTSFIQMGYNMIDMIWVGKA
                     GSKAVAAVGTAGFFPWLAMAFIMISKVGGEIKVAQSIGANNISTTKSYMKSAIEINII
                     LAIIYTISLIVLNKQLIGFFRLGDLEVITMSRQYLIIVALGMVFYFINPVFTAIFNGL
                     GNSKTPFRINTVGLITNIILDPILIFGWGFAPKLGVAGAAIATVFAQIVVTTCFIYII
                     VKSKEEYFKLRLFKEIETKYYKVLYKLGLPIAIQSGMFTVFSMLLGVIVASWGPVAVA
                     VQKVGSQIEAISWMTAEGLSVALGSFVGQNYGAKKYSRINKGCKIAITVSSVLGIITT
                     LVLVFAGESIFSIFIDESEAIEKGAVYLKILGYSQFFMCLEIITTGAFKGLGRTYIPS
                     IIITILTGARVPLAYFISRPEMLGLNGVWWSITLSSILKGILMISLFISLLKLGKLYK
                     ISE"
     gene            75413..76117
                     /locus_tag="PC545_000177"
     CDS             75413..76117
                     /locus_tag="PC545_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2454975.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVIANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADENLSSKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCEYKIAMSEREVQAVISNEECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            76317..76628
                     /locus_tag="PC545_000178"
     CDS             76317..76628
                     /locus_tag="PC545_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2454976.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            76650..77963
                     /locus_tag="PC545_000179"
     CDS             76650..77963
                     /locus_tag="PC545_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2454977.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQSGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            78060..79370
                     /locus_tag="PC545_000180"
     CDS             78060..79370
                     /locus_tag="PC545_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDF2454978.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEAG
                     KEKLEIVGNLAKRMVKKAGVDMKINLTLDRREALRDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVESDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNKGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPRFNR"
     gene            79396..80127
                     /gene="chbG"
                     /locus_tag="PC545_000181"
     CDS             79396..80127
                     /gene="chbG"
                     /locus_tag="PC545_000181"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDF2454979.1"
                     /translation="MTKIIINADDFGYCEAVNYGIISAHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKTLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDCDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLVSLKPVIEKIVTKYNLPIRGGFEYNLEYSK
                     VVPLIDSFYKENVSEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLEKNGLVISSYRDI"
     gene            80140..80457
                     /locus_tag="PC545_000182"
     CDS             80140..80457
                     /locus_tag="PC545_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2454980.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
     gene            complement(80569..81033)
                     /locus_tag="PC545_000183"
     CDS             complement(80569..81033)
                     /locus_tag="PC545_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2454981.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILDGTKTMVIRGAAGRKIPHSRVF
                     EDELLYFMEKGTAKISAKATVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIVEKIEDVVVGTSIPYNYEKSKF"
     gene            81303..82241
                     /locus_tag="PC545_000184"
     CDS             81303..82241
                     /locus_tag="PC545_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2454982.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNTEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPVATANVDSYETDKLPSYIREELKGVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSKL
                     FDKEEEDKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMVSMEGLTDIDADHIVVIATEADKKDLENSAIWAQIRAV
                     KDGNVTILDAAPFFSQSYNPIGKELLLESVKNELTK"
     gene            82369..83382
                     /locus_tag="PC545_000185"
     CDS             82369..83382
                     /locus_tag="PC545_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2454983.1"
                     /translation="MTKLSKKNITYLLILVSIVVLVFGIVLSITIGAKDMNLSTVIDS
                     LIKMEDGINMRIVKDVRLPRAIAAALVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFAISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVKWEGIKLLFVAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTKFVRFICIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            83388..84410
                     /locus_tag="PC545_000186"
     CDS             83388..84410
                     /locus_tag="PC545_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDF2454984.1"
                     /translation="MKNIIKINSKTLLVISILMVLIFITFLLSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALIGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSIWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            84425..85207
                     /locus_tag="PC545_000187"
     CDS             84425..85207
                     /locus_tag="PC545_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2454985.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPNSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMEDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDMV"
     gene            85219..86559
                     /locus_tag="PC545_000188"
     CDS             85219..86559
                     /locus_tag="PC545_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2454986.1"
                     /translation="MENQEALRHEKIWILLFRYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLVFLEEILFILGASKDSIFYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVIHYFIR
                     GKSNLKLKKCNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKQYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDSDLMDIAVRGISLNLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPVADFIAMIIVLLFLKREFKTSRV"
     gene            complement(86654..88933)
                     /locus_tag="PC545_000189"
     CDS             complement(86654..88933)
                     /locus_tag="PC545_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2454987.1"
                     /translation="MNFENTKNKKIIATLLLTLLLIVSLTCVTFIMNLNKVTHNETER
                     YLVEVSQGISTTINVKCKSIFRTLYSMGDTYFEINDSEKEVNRYLSKKLKLFEFDWLG
                     IIDSNGNFVSALKDTGNVKGQDFYRKALQGKETITPVKSQSGTYGILYSVPLYNGDKI
                     VGAVAAWNTIDTMRNLLSVESFHGEGFSQIIDQDGNFIVSSANKNAPKNVVNFFDMIK
                     HQGKQVDKNKLEEMKINLKKNKTGSMYYTLNDGISKAMHYLPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAIFINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDIEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLEPGE
                     LLCRVSNDQFNVLIKTIPQKEILKYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQDPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMLRNKYNIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHVIESIVNLAKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKKLEETSMDSSV"
     gene            complement(89270..90433)
                     /locus_tag="PC545_000190"
     CDS             complement(89270..90433)
                     /locus_tag="PC545_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2454988.1"
                     /translation="MKLMGYLRENGQFGIRNHVLIIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIDLTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKELASEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVSKMAREVSKEVRVEFGMSEITLALECGGSDPTSGI
                     ASNPSIGVASNLLVDEGGSSILSETTEVIGAEHLLATRFEDEEMKDKFLKFVSDVEKR
                     AIAMGEDLRSGQPTPGNKTGGLSTIEEKSLGCMYKAGNKPFKGALEYADIVPPDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEIFNEIIEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(90446..90721)
                     /locus_tag="PC545_000191"
     CDS             complement(90446..90721)
                     /locus_tag="PC545_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HDF2454989.1"
                     /translation="MNAIIHANEKDNLVTCVRPLVKGEKVSIDGKSYIVNDNIPVFHK
                     MATEDVKKGDVVYKYGEIIGIATVDIKTGDYVHVHNIESTRGRGDKK"
     gene            complement(90742..91716)
                     /locus_tag="PC545_000192"
     CDS             complement(90742..91716)
                     /locus_tag="PC545_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDF2454990.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVCPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGKPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIIALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVVLGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKSIFKTNKNIEVN"
     gene            complement(91987..93900)
                     /locus_tag="PC545_000193"
     CDS             complement(91987..93900)
                     /locus_tag="PC545_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2454991.1"
                     /translation="MKYGGIIMKEILVIAPTKGTYEKSIHIVKKNKYTNIDVVFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSNEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVVDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLSKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGGDKVIPIDVKIICASNKDLKNMMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSSKYNKTPLIITPNIKKALLEREYEGNIRELEGMMERAVIVESFDSIL
                     LEKVSNEYTNNIVDKDNSDFECSNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIEDDV"
     gene            complement(94077..95291)
                     /locus_tag="PC545_000194"
     CDS             complement(94077..95291)
                     /locus_tag="PC545_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2454992.1"
                     /translation="MNQEYVKGNPIFTNINKVPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CTSALEELDTFIELYGNKCDYAKRDTLLYTANKLEVKAIKEEYNLRKENGFDVEYIDE
                     STNPFNFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIENETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNNNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            95535..96917
                     /locus_tag="PC545_000195"
     CDS             95535..96917
                     /locus_tag="PC545_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2454993.1"
                     /translation="MLSKNIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNKDKVSVMKVAYKSAKEFSNDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPTQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDRPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDILMNIDMVGNNVKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            96929..98278
                     /locus_tag="PC545_000196"
     CDS             96929..98278
                     /locus_tag="PC545_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HDF2454994.1"
                     /translation="MELKNFKDILFKKALSEGFEEYEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     HYNDVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMLIGFVVPIIEDNGQKYDGIGYNIANSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILLNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIIKDIDEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEAIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            98428..98961
                     /locus_tag="PC545_000197"
     CDS             98428..98961
                     /locus_tag="PC545_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2454995.1"
                     /translation="MSDAHYDMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDETHRELSEMWINISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGNSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            99045..99854
                     /locus_tag="PC545_000198"
     CDS             99045..99854
                     /locus_tag="PC545_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2454996.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LRYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFNDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFEYSGHAIFYDELKLFNQKFLQFLEE"
     gene            100265..102310
                     /locus_tag="PC545_000199"
     CDS             100265..102310
                     /locus_tag="PC545_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2454997.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCNIDNLVYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIVHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KVIDINTVIEIFTKLNISIDKLHIIKEKYNEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASVDKLGKFIYCNKVFSNLVGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKNISAIQVLEDKIQNKLQSKGFVSKYTFESVVG
                     ESKIIKEKINIARKIATTDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYDEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDIPLISKYFLDEINSNKCFTEESMQALTLYEWPGNVRELKNLIYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSIETWNNKNILLGRNKL
                     QEILKEKDIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYVKIKGVI"
     gene            102571..103770
                     /locus_tag="PC545_000200"
     CDS             102571..103770
                     /locus_tag="PC545_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2454998.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     AKVKSIVDEKIFISLHEHPVLFPEHLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     AAKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGGNILR
                     VLKQVWK"
     gene            103822..104517
                     /locus_tag="PC545_000201"
     CDS             103822..104517
                     /locus_tag="PC545_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HDF2454999.1"
                     /translation="MDYLKLANHPLLWIASTVAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCMLEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            104530..105207
                     /locus_tag="PC545_000202"
     CDS             104530..105207
                     /locus_tag="PC545_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455000.1"
                     /translation="MSKVKSIYNEEYLPFKIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNISILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTMLIARAM
                     YKDKLSD"
     gene            105318..106919
                     /locus_tag="PC545_000203"
     CDS             105318..106919
                     /locus_tag="PC545_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2455001.1"
                     /translation="MIYDLILKNGFIIGGTGNPGFYGDIAIKDNLIAKIDSKINSNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKCWNNFTEYCDLISKSGISVNMGFLLGHGTIRWSV
                     MGGSKDRPPTEKEKNEITDIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     HDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVE
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGINGVMDALRNEEGRTLILKEAFIL
                     GDRGSIILLNTKDINMEKKSIREIATQKGIDEDKLLLDLLLDGDEELIFCLGGMYRAD
                     FPDKLHDDKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFPIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIDMDNYSYSRSEDIDFSKPQLLANGVEY
                     VIVNGKIALENGEITENKCGEVLKR"
     gene            107368..108657
                     /locus_tag="PC545_000204"
     CDS             107368..108657
                     /locus_tag="PC545_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2455002.1"
                     /translation="MRELLKVTLIVMLVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIVSVFNLSESIIYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLLVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRLLVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            108686..109366
                     /locus_tag="PC545_000205"
     CDS             108686..109366
                     /locus_tag="PC545_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDF2455003.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWMETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTDIYDSSVPIIDLYDEKTKNMIEFMERKGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PKNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     NSKECHNL"
     gene            109467..110006
                     /locus_tag="PC545_000206"
     CDS             109467..110006
                     /locus_tag="PC545_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HDF2455004.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKQGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFALLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSVYWTILTFIGVRYFQVYIVDSLYSLIIPYWPIVVTILLVTLF
                     VDEILTIKFLRGNKDIVER"
     gene            110182..111201
                     /locus_tag="PC545_000207"
     CDS             110182..111201
                     /locus_tag="PC545_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2455005.1"
                     /translation="MMGKIRFGIVGTSNIANIFLRAASRVKDFELVAVYSRNLEKARE
                     FGTLYGASIFFDDLEQMAKSKDIDAVYIASPNALHSKQTITCLKHKKHVLCEKSLASN
                     LKEVKSMIKTAKDNNVLLMEAMRITCVPNFKAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKTVKAVSHILQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSHENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            111489..112679
                     /gene="dltD"
                     /locus_tag="PC545_000208"
     CDS             111489..112679
                     /gene="dltD"
                     /locus_tag="PC545_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDF2455006.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTFPEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKRPINWSYERKKAIEDAKKRVGKNPLNIDKYYYK
                     EHFKDGIEQYKGRDKDVNLLTSKEFENYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQKIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            112800..114314
                     /locus_tag="PC545_000209"
     CDS             112800..114314
                     /locus_tag="PC545_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HDF2455007.1"
                     /translation="MKIIEGIKKYSNTDRTALMCNGDKLSYKDLNEYSDAISVFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDERNFGDINVIDMDKLNYIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPNLESMIFIGEALSKNLT
                     KELMSRFPNTRIINGYGPTEATVGVSVNDMTQKAIDDEKSLPVGYPMSNCKIKILDED
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGVKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNAVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            114314..115462
                     /gene="dltB"
                     /locus_tag="PC545_000210"
     CDS             114314..115462
                     /gene="dltB"
                     /locus_tag="PC545_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDF2455008.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYIYWGFLFASMLPIIINKISP
                     VTSFGIIGFIGISYLNFRTIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSRIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKDKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            115481..115711
                     /gene="dltC"
                     /locus_tag="PC545_000211"
     CDS             115481..115711
                     /gene="dltC"
                     /locus_tag="PC545_000211"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDF2455009.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            115928..116713
                     /locus_tag="PC545_000212"
     CDS             115928..116713
                     /locus_tag="PC545_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2455010.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNNTNLDKKQSIAKYAAKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPISIDAMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKAYIKNSKESFVLCDSSK
                     IGIASMYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            116992..118902
                     /gene="phnW"
                     /locus_tag="PC545_000213"
     CDS             116992..118902
                     /gene="phnW"
                     /locus_tag="PC545_000213"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDF2455011.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNIWSDKFGKVPTEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVVETIEKLRKNGLKIGSTTGYTREMMNIVEPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTQ
                     EEVENMDKEELKAKMSIVSKKFKEAGAHFVIETMAELEDILIKIENETIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNNLVQDVRRRLVSLATQNTDKYTSVLMQ
                     GSGTFSVEAIIGSTISKDGKLLVIANGAYGKRMKDICNYLNIQFVDCTFKDIEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKIYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEKLSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKDNGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIEKFINR"
     gene            119261..120082
                     /locus_tag="PC545_000214"
     CDS             119261..120082
                     /locus_tag="PC545_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455012.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNISGTGKIEGSVKCSKFDSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGNINSVEIDTSGIINVEG
                     SIKSDIIHGEGYLKANENIDCENINLSGILECNGFLNCEEVNISLNGTSNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYTESKLFANIIEADEIS
                     LENSEVKVVRGKNIKIGKGCYVESLEYSESIEIDEDSIVGEIKNISEKIKLKKDK"
     gene            120123..120890
                     /locus_tag="PC545_000215"
     CDS             120123..120890
                     /locus_tag="PC545_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HDF2455013.1"
                     /translation="MEKGSIKILGEGFISSGEYENIKIVGSSKSKGNVKSNEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLNIEGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVNENMYAQDIKIYGEMRNKSNIECEDIKVYGAIKCDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDAFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSISLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            120898..121518
                     /locus_tag="PC545_000216"
     CDS             120898..121518
                     /locus_tag="PC545_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HDF2455014.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSIITFEELKLIVNIIEENFTSIYNESGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(121818..122405)
                     /locus_tag="PC545_000217"
     CDS             complement(121818..122405)
                     /locus_tag="PC545_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2455015.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENISISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHIGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(122491..122928)
                     /locus_tag="PC545_000218"
     CDS             complement(122491..122928)
                     /locus_tag="PC545_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2455016.1"
                     /translation="MEDKYIVHFISKTKASMIKFIENKLSKNGLGELIPTHGNILTAL
                     YENNGILTMKEIAAKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            123032..124030
                     /locus_tag="PC545_000219"
     CDS             123032..124030
                     /locus_tag="PC545_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HDF2455017.1"
                     /translation="MNKKFKSLYITEEEGNFRRNIVEREVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVKLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKNMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKEVLLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGKHVGRTVVDLEKI"
     gene            complement(124150..125076)
                     /locus_tag="PC545_000220"
     CDS             complement(124150..125076)
                     /locus_tag="PC545_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2455018.1"
                     /translation="MSKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMEGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSMGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNTYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            125462..127066
                     /locus_tag="PC545_000221"
     CDS             125462..127066
                     /locus_tag="PC545_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2455019.1"
                     /translation="MKAKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKSVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETRDPEGGHIGRVEGSEEPNGYLEENAFFNVASKIKQPSSNEIFNSIEKAQNIYLS
                     YGITTAQEGLMEENQFNILKAMANQNKLKMDVVGYVNLKKSKSVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSRPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAADQLIDAFEKVLNLKEQSSENNIRPVMIHAQTVRADQIDDMKVINMIPSY
                     FVAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAV
                     NRITKKGIQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPL
                     TIDPMKIKDIKVLQTIREGEVLYSLK"
     gene            127882..129153
                     /gene="serS"
                     /locus_tag="PC545_000222"
     CDS             127882..129153
                     /gene="serS"
                     /locus_tag="PC545_000222"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2455020.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKNFNLDEVVKLDDERRKTLQE
                     VEVMKNELNTASKNIPNLIKEGKDVENEKIKLKELSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPEVPQGETDEDNVEVRTWGKTTTFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGMDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYVHTLNGSGLAIGRS
                     VAAILENYQQEDGSVVVPEVLRPYMGVSVIK"
BASE COUNT        49847 a        14186 c        22292 g        42888 t
ORIGIN      
        1 tgctataaaa gtttgtttaa taaattaaat aaagaaaaga ggagaataaa atgtacaaca
       61 tagatgatat gatatataat tgtccagtag aagctttatc aaatatttta ggtaaaaaat
      121 gggtggctat aattatatgg aaaatacaag aagataaaaa acgatttgga gagctacaaa
      181 gagctatacc agactgtagt aagaaaatgt tagttcaaca actagatttt ttaattgaac
      241 aggggattat agtcaaccaa aaaaagttgg ttaataatgt tgtagagtca acatattttt
      301 taagtgaatc aggattacga ttgcttcctg tcatggaaaa aatgattatg tggagcaata
      361 aaaatttaaa ttgtgataaa tactaaaatt tactctgtaa gtaatgaaaa tttaattaag
      421 ctatgagtag aaaaatgtat taaaattaca taatttttta gttagatttt aatttagaaa
      481 taggattgta atattggaaa taaaataagg aggaatttta atttgcaaaa tgtaaattta
      541 tttttagtat ttatagaagg tatagtatca ttcttttcac catgtatact gccaatttta
      601 cctatttatt tgagtatatt gtcaaatagt agtgtagaaa atttaaaaga aggaaagacg
      661 agttttatag gaagttcttt atttaaaaat actatatttt ttgctcttgg aatctcaaca
      721 acattcttta tattgggttc atcagtaaaa gtattgagta tgttttttaa tgaaaataaa
      781 gatttaataa tgtttattgg tgggattata attattatca tgggattatt ttacatggga
      841 ataattaaat cttcaatatt gaatagagaa aaaagattta atgttaagtt taaagaaatg
      901 aaagcaataa cagcttttat attaggtttt acgtttagtt ttggatggac accttgtatt
      961 ggtccaatat tggcatctgt attagtcatg gtttcaagtt caagtaatca tcttagtgcc
     1021 aatcttttaa tagcagtata tactattggt tttattttgc cattcataat tacagctatg
     1081 ttttacagta aactatttaa aactatagat aagataagat ctaacatgga aattattaaa
     1141 aaaataggtg ggattatact tatagtatca ggaatattaa tgatggtaaa tggatttgga
     1201 agtataagca aacactttaa tacgtcacaa aatagtaaaa ttgaaagcaa gcaagaagaa
     1261 aataagcgag aaaattctac tgataaagaa gaaaattctg atggtaatga tagtcaaaaa
     1321 gactctaata atgacaataa tgataaaggg tctaatgatg aagatagaat aaagtctatt
     1381 gattttactt taacagacca gtatggtaaa acacataaat taagtgatta tgaaggcaaa
     1441 gttgtattct taaatttctg ggctacatgg tgtcctccat gcaaagaaga aatgccttat
     1501 atagaacaac tgtataaaga ctataataaa aataatgatg atgtagtaat tttaggagta
     1561 gcttcaccta atttaggtag agaaggttca agggaacatg tagttaactt cttaaaagac
     1621 caaggttata cattccctgt tgtacttgat gaagatggag cattagcata tcaatatgga
     1681 attaatgctt ttccaactac atttataatt gacaaggaag gatatgtaac tcagtatatt
     1741 ccaggagcta tggataaggc aactatggca tcatttattg aaaatcagag aaataaataa
     1801 aagtatttaa gtataagttt ataaatcaat ttaaggttac aaaaaagtgc ctaattgtac
     1861 cacctaaata atcttgttat agtaaaagta taaacaatac aagaaagggt tgaattttat
     1921 atgaaaaaaa catttgatgc tataataata ggatttggaa aaggtggaaa aacattagca
     1981 ggggatttgg caaacagggg attaaaagta gctttaatag aaaagtcaaa caagatgtat
     2041 ggtggaacat gtgtaaatgt ggcatgtata ccaacaaaaa gcttagaaaa ttcagcaaac
     2101 agcgtaaaaa caaagaatat aaattcttgg gatgaagtac aagctgaata tgagaaagct
     2161 atagataaaa aagaaacttt aattacaaaa ctcagagaag ctaactataa taaattaaat
     2221 tcaaatgaga atgtaactat attcacagga atgggaactt tcattgatga aaaaacagtt
     2281 caagtcaaaa ctgaaaatga aatttatgag ttagtagcag ataatatatt tattaatact
     2341 ggttcaagac catttatacc taatattaag ggaattgaaa ataagaatat agtatatgat
     2401 agtgagtctc ttatgaatct aagaacttta cctaaaaaaa tgacaattat aggagctgga
     2461 tttataggtt tagaatttgc tggtatttat agttcatttg gagcagaagt tacaatatta
     2521 aactctaata atggcatttt accaaatgaa gatgttgagg attcagaaga aataataaaa
     2581 ttacttgcaa agagaaatgt aaaaattgtt aataatgcaa atataaaaga gattaaagaa
     2641 gtttctgaat tagcaatagt agaatatgaa gtagatggta aatctaaaga attaacaagt
     2701 aatatgatac ttgtagctac aggaagaaaa gcaaacactg aagggctagg gctagaaaat
     2761 gcaggtatag aattaaatga aagaggattt attaaagtct cagaaactct taagactaat
     2821 aaagagcata tatgggctat aggagatata aatggaggtc cacaatttac ttatatttca
     2881 ttggatgatt atcgcatagt tattaatcag ttatttggag ataagactag aacaactaat
     2941 gatagaaaga atatcccaaa ttctatattt ataagtccag cattttcaag agttggtctt
     3001 aatgttaagc aagctaaaga aaaaggatat gaagtattag tggcaaagat gccagtagaa
     3061 gcaattccaa gagctaaaca gattggcaag gcagatgggt ttataaaaat agtgatagat
     3121 aaaaaatcca ataagatact tggagctagt atgatatgtg aaaattcaag tgagattatc
     3181 catcttattc aattagctgt agatttagaa gtagagtata catatttaag agaccgtgtt
     3241 tatgcacatc caactatgac agaagctctt aatgatattc tatctcctaa tatgataaaa
     3301 gaagtataac ataagtttaa tatataaacc taaaattatc attataagta gattttagaa
     3361 agaggctatc tcaaaaaata gagataagtc tcttttttac tgtttaaatg ataaaaatat
     3421 atttgatttt ataaaagaag attatcttat gaactaaaaa gtttatttta aaacactacc
     3481 tatttatgtt ataaacttat aaatataata tagaatctat tattctgtat tattaagtgg
     3541 ggttaagcgt tataattaat tataaatatt gaataatcta aatggataag ataatggtat
     3601 taaaaattat cttaaaaggt gaaataatct gtatttaatg tagtacagtt atatctttaa
     3661 agcaaggtgg tgaaatgata taaataagat atctaaaaga gggtttgatg atagtgataa
     3721 aagatggttt tcaagtaaag aattaacaag acttataaag gctaaagaag aaatagaatg
     3781 gttattaaat agggattaca aaatagaccc agtcgttact tttgttggtg atagatatca
     3841 gttttctata agacaaagag atgcattaaa aagggcagtc tgtactgatg aaaaaaatat
     3901 tataagacaa tcaaagaggc tttctctaga taaaatcaaa gaagataccg taaatataga
     3961 tggctttaat ttgataatat ccatagaggt agcattatct ggtggtactc tagtaatagg
     4021 aagtgatggc aatataagag atttagcagg tcttagggga acctataaga ttatagataa
     4081 aacagaagaa gctttgaatc tgataggtaa attttttaat aaatacaatg cacagaatct
     4141 taaattttat ttagatgctc cagtttctaa ttcaggaaat ttaaagtata ggatattaga
     4201 gcatgccaaa acttggggaa ttgaaactga agtagaatta gtaaaaaatg cagatgttgt
     4261 attggaaaaa ttagatagag tagttagtag tgatgcagtc atagttgaca agtgtataag
     4321 ttattttaat gtggctagag gtataattga agagtatatt aaagaatgta atatcataaa
     4381 tttaaataaa tagataaata taggtgagaa tatataagta tatgtagcaa tattattagt
     4441 aatacaagta aagtttgaaa ataggagaac gaatacatat cgaatttcat aaaatgttgt
     4501 aaaaagacat atgagaggat ggatatgtgt gaaattaact aaactaaata ttaaaaaata
     4561 tagagcatct atatataaat atgctttagc aaatgtaaat ttgaggtctg ctaaatctac
     4621 caattcaagt atcataacag taatacctca gggagctaaa atggaagtat tggatgaaga
     4681 agatgactgg attaaggtga tgtacaattc tcaagaagga tatgtgtata aggatttggt
     4741 atcagtaagc gaatatgctt ggagtaactt aaacttaaga gaagataaat ctacaacttc
     4801 caatataatt acagtaattc ctgaaaaatc tagggttgaa gtccttcaag tagatggaga
     4861 ttggagtaag gttgtatatg atgataaaat aggatatgtt tttaattatt ttttatcaat
     4921 tgatgggaat aaaccaaatg aattagatta taagtatttt tatactgaca tgattaaatt
     4981 tgttaacgaa aataatatta aaagtactag cgattatcta attgtaacag accttagaaa
     5041 taaatatact tatattttta agaaagataa tggaggctgg ggacaacttt acaagtggca
     5101 atgtactata ggtaagcctg agactccaac tataacaggt atattttata taagtggaag
     5161 aaaaccttct tttggaacgg atgagtattc agttaaatat gctactagaa ttaaaggtgg
     5221 atattactat cattctgtgc tatatgattc aacaggaagt tatataatag atggcagact
     5281 tggtgaagct cttagtcatg gatgtataag attaagtacg gaaaatgcaa agtggatata
     5341 tgataatata ccagatacaa caacagttat aattcattaa gaaaaatttt gagtttttta
     5401 ttgggataat gattcttaaa ttaataggat tatatattaa cttctcataa agagtactat
     5461 ttaaaaagtg taataagaga atgctattaa caaaacaaag atattttgag gcactctctt
     5521 ataaatatac ataattaaga tattttcata aacaattcta taatcttatc tttatcaaag
     5581 aaaacaggat tagcaccagc acaagcatct ttcatagagt tttctgcaag caattcaaaa
     5641 ttaggattat caacacctac atcttttaaa gattttggga ttccaacctc ttcagaaaga
     5701 gcttttatct tatctattac aaagtcaaca cactctttat ctgatttatt atttacatcc
     5761 attccaataa cttcagctat tggtcggaat ttggcagggg ctgatttagc attttcctcc
     5821 tcaactattg gaagtagcat ggcattacat actccatgag gtaaatcata taaacctcct
     5881 aattgatgag ccatagcatg tacatttcca agacctgcat tactaaaggc aattccattt
     5941 aaaaaacaag cataacacat ttgttctcta gcttctatgt cattaccatt tttaacagct
     6001 cttggaaggt atttaaatat ctgctttata gcatataagg cagttgaatc tgtaacatca
     6061 taagcaccat tagctactac agcttctata gcatgagtta gtgcatccat accagttgct
     6121 gctgttaaat tagatggttt gctaatcatt aattctgggt catttactgt cattgtagct
     6181 agtgaattgg tatcaaccat tatcatcttt acatgtctgt cttcatcagt tataacatag
     6241 tttattgtaa cttcagcaga tgtaccagca gtagtagtta tagctatgat aggaagacat
     6301 tttttagaag ttttgttgat accttcatag tctctaatat ctcctccatt agtggctaat
     6361 atagagatag ctttaccaca gtcttgtgga gaaccacctc ctatagttat aacgaagtcg
     6421 caattttctg atttcaatat tttaagtcct ttattcacat tagttacagt tggattttgt
     6481 ttaacttcat cataaacaac aaagtctaca tctatctgag ataaaatatc tgttactttt
     6541 tttacagtac catttgaaac tagaaattta tcactaacaa taaatgcttt tttagttcct
     6601 aatgatttaa ttgaatcttt agcttcatta agacatcctt tcccaagtaa atttattggt
     6661 ggtacataat aaatgctcat aactaaaatc tccttttatt ttcattgatt tttatgtaaa
     6721 aatatatatc taacaatttt cactaattta ctcataatat atcgtatctt agattgagat
     6781 agggttgctg gttcttatat aagaacttaa ttcgtaatta cgattatgat tagaatttaa
     6841 catagtattt ttatcatgtc aataaaaata taaaaaatat tataaaaaaa aacaaagtta
     6901 ctgtagttaa atcagcaact ttgtttttat agtataaatc aaataaatac attagtttaa
     6961 aacaaaattt ttaattatta tgttttgata tcttccatat acagaatatt aatctaattt
     7021 attacaatgt cttcattacg ttcttccatc attgattgaa tatttttctg agcatttaag
     7081 agtaattttt ttatcagttc tattttttcg tcattagctc taaataaagg cagtgttaca
     7141 ctaacagcag ctcttataca tccatttttt cttattggta cggcaagaca ttttaggtgt
     7201 tccattgatt cctcacactc tgaagctatt tgtgttcttt gaacttcaag aagctggtcg
     7261 tataaaacat caaaatctgt aattgatttt tctgtaaaac cttttagacc atctggatat
     7321 aagcttatta gtttttcctt gtcaaaatca cataaaagag cttttccaag acctgtacaa
     7381 ttagcaggta ttttttttcc aacataagaa ataagtctaa ttggttctgg agaatctact
     7441 tttccaatat aaagaacttc attattgtca agtataccaa gctgacaagt ttcagaacag
     7501 gaattaacta tattagtcat ttcgtcaatt atatagttaa aaatagttct tttttcaaaa
     7561 aatgaagacc ctaatgtgta agaacgtatc cctatagaat atttggatgt gtttttatca
     7621 atagtaatgt attttctagc attaagtgta tgaactatag gaaagatgct actttttgga
     7681 gcattgataa catctgctat ttctgtaaga gtatatccat tttcggattc tgctaatgct
     7741 tctaaaatat ctaatatcct agctgttggg cgatgattat ctgaagtcat aaatttcccc
     7801 ctctttttat taatttaaag tattagaata tgttgatttt agcacactat acgatatttt
     7861 acaatgtaga aaataaatat gtaaatttat atataaataa gctcttgatt tagatacaga
     7921 tagatatttt tttaggctgt acattaagga ataactaatt tccaaaatat acctaaagaa
     7981 agtaagtgat atttagttat ataatttaag taaatatatg caatagtgat ttttatatgt
     8041 aaatatcaaa atattatgaa cttatatttt atctattttt tacactgtat aaaaacttta
     8101 tagttttgaa tactatataa ttttaaatac gctgtatttt tgcatataag gaagtcattt
     8161 gataatatac aaggtattat cagaaagttt gaaaaaattt tgacaaaaat ttattttttt
     8221 tattgactca taaataggaa aatgctatca ttaattcaaa gattcgtaat taaaaatcta
     8281 gttcttaaat acgaaccgaa acggccgtta gaattaaagc taaaaactta ataagagtaa
     8341 ttaaatatca tgttttagga ggattaactt tatgataatg gattttatta aaaaaattcc
     8401 tgcaggtatg atgatagtgc caatgtttat agcagctttc attaatacct tttgcccaga
     8461 tattgtacaa ataggttcgt ttacgacagc agtattttca agtgttgggt ctgcaactat
     8521 aattggggtt caactggtat gcttgggaac tcagcttcaa tttagagaaa tgccaaaagt
     8581 tttaaagaga ggtggtatac ttcttggttc taaatttgct ataggtgctt tgataggtat
     8641 acttatagga aagctatttg gaatggatgg tgtccttggt ctgacaacat tggctgttat
     8701 aagtgcagtt acaaatagta atggtagtat atatctttca ttgatgactt catatggaga
     8761 tgaaacagat tgtgcaacta tgagtttact tactttaaat gatggaccat tttttacatt
     8821 aatagctctt gggacttcag gtcttgcaaa tgtaccattg ttgtcattat tggctgcaat
     8881 agtgccaata cttgttggta tgataatagg aaattttgac aaaaaaatga aagagttttt
     8941 agagccaggg tgtaatttac tcgtaccttt tgtagggttt gcacttggta gtagcataaa
     9001 tcttgcaagt atactaaaag gaggtttatc aggagtatta cttggattaa tatctgtatt
     9061 tgtaggaggt atatttatag tattctgtga taaatttata ggaaaacgtc caggatatgc
     9121 aggatgggct gtagctacaa ctgctggaaa tgcaattgct gtacctgctg ctgttgcatt
     9181 agttgaccca tcatggcaac catatgttgc aacagctaca acacaagtgg cagccgcagt
     9241 agttgtcact gcaatattag tacctcttat aactagttgg tgggctaaga agtatggatg
     9301 tcctaagttc ccaaaacaaa atgaggatgg tacaataaca gcttaattta tattacagtg
     9361 agttgttggt aaaataataa attctttaat tagattttag gatattaata aaattatatt
     9421 atttataaag gagagcaaga ataatgataa atgcagtgat aattgatgaa aaagataatg
     9481 ttgcagtagc tattgaagca atagctaaag gtagtgaaat tagttttaaa ttaaaagaca
     9541 aaacagtaaa aacgattaca gcactagatg atataactat ttatcataaa ttggctataa
     9601 aagatatggc caaaggtgat aaagttacta aatatggtga acatatagga gaagcaaatc
     9661 aggaaataaa aagtggtcaa catgtacata ttcataatgt tagaagtgtt agagaggatt
     9721 tggataaaca agttatgact aatatataaa ataatattta tagagttata aaagttgtat
     9781 tcattgcttt tagtgtcaaa tagtttttaa ataggttttt agctgtttga ttagtgagtg
     9841 taataaaaat tataatatat aaaaattata aaaagtatat agactatttt aggaggaaat
     9901 cagtatgaaa ttttatggat ataaaagacc agatggaaga gttggtataa gaaaccacat
     9961 attaatcttg cctacgagtg tgtgtgcatc agatacaact agaataatag catcacaagt
    10021 tcaaggggca gttaccttta ataatcagaa tggatgttca caagtacatt cagaccaaca
    10081 attaactatg gatgttatgg ctggttatgc agcaaatcca aatgtatatg gtgtaatagt
    10141 tgtatctttg ggatgcgaaa attgccaaaa tgatttggta gttgatgcaa taaaagagag
    10201 aacaaataag ccaataaaaa ctcttgtaat acaagaagaa catggaactt taaaaactat
    10261 agaaaaagct gttcgttatg ctagagaaat ggcacaagaa gcttctttgc ttagaaaaga
    10321 agaatttcca atatctgaac ttatactagg aactgaatgt ggtgggtctg acccaacaag
    10381 tggattagca gcaaatccat taataggtga attaagtgat aaacttgtcg atttaggagc
    10441 aacatcaatt ttaagtgaaa ctactgaatt tataggtgca gagcatatac tagctaggag
    10501 agcagtaaat gaagaagtaa aagaaaaaat attacatata gtacatagat atgaaaattc
    10561 attaaaattg gttggagaag aagttagaga aggtaatcca tctcctggaa atatagctgg
    10621 tggtttaaca agtttagaag aaaaatcact tggttgtata cataaaggtg gtcatagaca
    10681 aataagtgaa gtatatgatt atgccaaaca aattgataag aaaggccttg ttataatgga
    10741 tacccctgga aatgatgctt catctgttgc tggtatggta gcaggaggag cacaaatagt
    10801 ggtatttagt acaggaagag gaacaccatc aggaaatcca atagcaccag taataaaaat
    10861 aactggaaat aaaataactt ttgcaaatat ggaagataat atggattttg atgctagtcc
    10921 agttatatat ggtccacaaa caatggaaga attaacagat gatttgttaa atatggttgt
    10981 agatgtagca aatggaaaac aatcaaaagc agagtccctt ggatatactg aaatggcaat
    11041 agctagagta tgcaactatg tataatcttt aagtaagaaa ataaatacat agaattttaa
    11101 taaagggtga gattatgaag tttgtaagtt ttactgaagg agaaaatgaa tgtaaaagaa
    11161 ctggagtttt ttctaaagat gaattattta ttatagatgt taactcttta aatctcagta
    11221 gaaattttaa ggatttaaat gaattaattc aaaatgtatc tagtgaagat attattaagt
    11281 tgaaaactat tataaatgaa agtacattct ataattatga tgtttttaaa agagagagat
    11341 gtactgtaca tgttcctata gaaaaaccaa tacatgatat cttatgtgtg ggagtgaact
    11401 ataaagacca tcttgaagaa actcaaactc actttgatga tagttttgaa gaaccacaaa
    11461 aaacggttta ttttacaaaa cgtgtgacaa aggcaatagg tccagaagat gagatagatg
    11521 gtcattttga gataaataat caactagact atgaagttga acttggagta gtaataggta
    11581 aaacaggtgt taatattaga aaagaagagg tagaagatta tatctttggt tacactgtta
    11641 taaatgatat ttctgctaga aagttgcaaa aagaacatgt tcaatggttt agaggaaaag
    11701 gattagatac atttacctct ttaggtccat gtattttaca taaaagtgca gttccttttc
    11761 ctataaaact taaagtatgt agtagggtaa atggtgaaga aagacaaagt tctaatacag
    11821 gattattcat atcagacgtt tcagacatag tttcagaact ttcaaaaggt atgaccttag
    11881 aggctggaga cattatagct actggaacac cttctggagt agggatggga ttttcacctc
    11941 ctcgatatat gagtagtgga gatgtagttg aatgtgagat tgagagtatt ggaattttaa
    12001 aaaattacat taagtaactg ttttaatata attaatatta aagctaaatc tgatgtatta
    12061 atgaacatat taaaataaat tttaggagtt taaattatga aagcagaatt tttgacacca
    12121 gttgttacaa tatttgatga agaaggaaat ttagataaag aaggtaataa aaaagtgtat
    12181 gaccatctta tagatggagg tgtagatgga ctagtcataa tgggaagtac tggagagttc
    12241 tttaatatgt ctatggatat gcaaaaagaa cttatagatt tagtaactag tcatgtaaat
    12301 aagagagtaa aagtgtttat aggtacaagt cgtatgtgta taaatgaatc tatagaactt
    12361 tcaaactatg cacataatag tggagcagat ggtgttatga taataagccc ttattatttc
    12421 tctcttgatg atgatagtat tgagttattt tatagtgaaa tagctaagaa tacagaggct
    12481 gaaatatatt tgtacaatta tccagataga actggttatg atttaagtcc agaacttaca
    12541 ttaagacttg caagaaaaca taaaaacata gttggatata aggatacagt aactttgatg
    12601 ggacatacca gagatttaat aaatactatg aaaatagagt tcccagaatt taaggtttat
    12661 agtggttatg atgaaaactt tatacataat ctaatgtctg ggggagcagg ttgtataggg
    12721 gggttatcaa atttaattcc agaagtttgc tccaaatggg ctgaagctta caataataga
    12781 gatatggaga aggttataga aattcaaaaa tatattaata aagcaatgga tttttactta
    12841 ataaccaatc catttatacc agcaatgaaa aaagctatga atatcagagg gttacaaata
    12901 agtgattata tgactgctcc atttgtaaaa ccaaatgaaa aacaagtatg tgaaattaaa
    12961 aaattgatga atgaattaaa tatatgttaa atcaaccttg caaagaggtc aacaataaat
    13021 tacaattatc ttaataaaaa ttaaattaaa ataatactag ggtactatat tagtttaata
    13081 gggaaagtga tgaaaattca ctacagcccc cgctactgta tacggatacg aaatcaaaat
    13141 ccactgaaat ttataaaaat tttgggaagg gtgatggagg aaaagccgtg agtcaggaga
    13201 cctgcccagt attatataag ttcaatttag ggtggagatt gtaatttata ataaaatgaa
    13261 gtatcaattc gtttttgtaa agttgatata tgaacttctc cttattaaat taggagaagt
    13321 tttttatttt attaaaaggt tatacttatt aaagatgact ttaggataac tttaataagt
    13381 atactttaaa aagcatatat tattaaagtt ttttagccac tcataaaaaa taagatattg
    13441 gagtgaaaga gatgaaattt aaatcaaatg ttttaaagca gtttttttta attgctatgg
    13501 taatttttat ggtaggaggt cttatagggt gtagtaaaag tgacaaagag aataaacaag
    13561 ctagtactgg taagaaatta gaattaaaat atgctagtgg atttagtgtt gaagatttag
    13621 aagatggaat taaaaaagta actgatggag ataatcgaga actgatttta atcccaaaga
    13681 agattaaaat tcctgaaaaa tataaaaatg ctaatatagt tagaacgcct gtggacaatg
    13741 tattaatagc atctacaaca caggcttgtt ctctgagaac aatagatgaa ctagatagta
    13801 taaaagccgt aacaacagaa gagcagtact ggactattaa agaaattaaa gatttaatga
    13861 aagatggaaa aatacattac gttggttcaa atttagcacc agattatgaa aaaatagtag
    13921 acttaaatcc tgacttaact atagtgagta gtggtttatc agaggctgat acaaaattta
    13981 ttgacaaatt aaaagaatta gggcttaatt atgttgtaga caatgagtat aaagagcaaa
    14041 atccatttgg aagaatggag tggacaagtt taatagctgc attttatgat aaagaagata
    14101 tggctactag tgaactaaat aaaactgtac aaaaggtaga agaagtaagt aaaaaaataa
    14161 aaaacaaagc taaacctaaa gttgtatggg catctgtata tgaaggaagt gcttatatag
    14221 ctcctaaaga ttcatatgtg gataatatga taaaaatggc tggtggaata aatgcatgtg
    14281 cttcaataga ttcaaatgaa gggcgagtaa gtattgaaca attgcatgaa gttgcaaaag
    14341 atgcagatat atttgtatac tcatcaagtt cagattatgc accaaattta gattctataa
    14401 aatctagtgc acctgtattg gctgatttgg atgttgtaaa aaacgggaac ctatgggtgt
    14461 ttgctccaga ttattatcaa tcagtagata agacggacga attaatagta gatttagttg
    14521 agatgttcca cccaggtact acaggtgaaa aaatcaaaca ttatataaat tatgataaat
    14581 agtacagaga ggacacaaaa agataggttt tatagatttt agttaatata gctttgctaa
    14641 gaggagtata caaatggatt ttatcaataa aatgagaata aggaatacaa caatatttat
    14701 agtattaatt ataagtatga tacttacttt tttagtgtgt gtagcatttg gctctgtaaa
    14761 aattgaattt aaagaagtaa tagatgttgt attaagaggt atggataata cagaacatta
    14821 taaaataata tacaatataa gaatccctag agtacttgca accatggtag gtggagcatg
    14881 cctagcagta tcaggtattt tattacagat attttttaaa aacccaatag ttgaaccgta
    14941 tatattagga gtatcttcag gttctacttt atcagtagct ctcatgattt tgggaggata
    15001 cacattggga tttgagagtg taaattcaat gagtatattt gtagcagctt ttataggagc
    15061 tttaattgta atgtctatag tcattttatt tgcaaataag gtaaagaata taacaactct
    15121 tttaataata ggaattatga ccggatatgt atgtagtggt ataacaaatg tgttacaatc
    15181 atttgcaaat agtgataaat taaaagaatt tactatgtgg tctatgggaa gtttttctgg
    15241 atttacatgg gaaaaggtag aaatacttat aatagttggt atattaggtc ttttgatgac
    15301 tgtattcata ataaaacctt taaatgcttt tttactagga gaagagtatg caaaaagtat
    15361 tggcataaat ataagatttt ttagagtttt gatagtattt atatctagta tattagcagc
    15421 aatagtatca gcatttgcag gacctatagg gtttattgga cttgcagtgc cacaaatttc
    15481 aaaaatgata tttaaaacat cagataatag agttttgata ccagtagtaa tatttttagg
    15541 cagtatagtt acagctcttt gtgatttaat atcaagagta attttatcac cagtagaatt
    15601 accaataagt acgataacat ctttattagg agctccaatt gtagtaatgt tattggtgaa
    15661 aaggaaggat agggcttaga catgttgaaa actaataatt taagtgtagg atacaataac
    15721 aaagttgtta taagtaatat aaatgtagaa gtaaaaaatg gagaaatact atgtcttttg
    15781 ggaagtaatg gagctggaaa aactacccta ttaaggtcct tatctaaact tatatcacca
    15841 ataaaaggtg aaatatattt gaatggtgta aacataaact gtatatcaag aaaagcatta
    15901 tcaaaaaaaa tggctttagt acttacaaat agattattag gtgatttaat gactgtacaa
    15961 gatatagtca atataggaag gtatccatat acaggatttt ttgggagtct atctaagaaa
    16021 gatttaataa tggtagatga agcattggag tctgtagatg ccttacattt aaagaaaaga
    16081 tattttgatg aactaagtga tggagaaaaa caaaaggtgt tggttgcaag ggctcttgtt
    16141 caagaaccag aaataattat attagatgag cctacaactc acttggatat aaaacataga
    16201 ttggaactca taaacatact gaaaaaatta agtaaagaaa agtcaatatc agtaatactt
    16261 tcattgcatg aaatagacat agcattaaaa agttgtgata aagttgctct tataaaaaat
    16321 aataaagtca tagcttatgg tcagcctgaa gatgtagtag atgaggatat tataaattcg
    16381 ctttatgaat tggatgataa aaactttaat agtcttttag gttcagtaga aattagtaat
    16441 aaatctaaaa atgaagtttt tataattggt ggtgggggta aagcaacacc aatttataga
    16501 gcttttacta aaaaaggtat aggtctgtat tctggaataa tacatgaaaa tgatattgat
    16561 tatgaaattg gtagaactat ggggataaag atgtttacag aaaatccatt tgagcctata
    16621 agtgatgaga gtttcaattt ggctattaga aatttaaatg attccaagat tataatagat
    16681 acaggattta gtgtaggaga gacaaataag aggaatatag atattataaa agaggcttta
    16741 aaattggata aaaaagttta ttcttttaga aatagagatg agagtaagaa gtattatgac
    16801 agtttggata gtaagataga acatatagat aaagtttctc aaataatcaa tagtgcagat
    16861 ataaataatt tattataatt tatgaaataa aatgtactta tgtgctatag aaaaaagagt
    16921 atagattata atttttttga aattacaatc tatactcttt tgttattttg tttatgtatt
    16981 tatatattta taaaacttta gcattttata gataaccaat agcttcctct ctagttaaaa
    17041 aaaagtgaat ccctgtagtc gattctttcc atctattttc attaaaatct tcagctacaa
    17101 tcgtttctcc tactctatat ataaaatttt catcagcata tgcacgagca cttttaaaag
    17161 attctttacc atctatacta gtaattgaca gtacttttgc cttatcacat ctacaagcgt
    17221 ttgatgtagc agacactctt tttgcgtcgc tgggtattaa taattgtacc attctaaaat
    17281 taaaacattt tttatagcct ataaatgctc ctgtttcagg acaatacaat ttaaaaaaac
    17341 gggtactttc atctgatatt actccattta aagtagcatg ttctaaattt gaattttcta
    17401 aattagctcc tctaatatct gagttagata agtcacaaaa tctaaaactt gcaccactta
    17461 agtctgcatt ttttaatata gaatttttta atgaacaatc cgaaagataa gaattattaa
    17521 aaatagagtt ttctaaatct gctttttcta aatttaaatt gataaggtca gaccacgaaa
    17581 agtcaatatt acttaagcag tatcctctta agtccatatt ttctaatctt aattctttta
    17641 aatctgcctt ttctccagca ttattgtttt taagccataa ataatgtttc tcaagtgttt
    17701 tttctaattc ttgtttagaa atagttttca ttaaaatcac cttcataaat ttatttttat
    17761 atatgtagta gcaaaatagt catatattta attatataac aataaataaa aaacttctaa
    17821 ataatatata aaatatcatc ttgaaagtac ttttttcaat atatcagaat atatttttaa
    17881 gctatttaga gataatttaa atttttaaac aggacataat aagcttgtat tgttttatat
    17941 ttgagttgat aaaaatttct ttaaataaaa ataatgtcaa ttataaagta ataaaagttt
    18001 agatataaaa aaatttgtgt atatactata tgtagtatat aatagataaa gcactgacta
    18061 tatgtagtgc ttttgttttt gaaaaatact tattgcattt ctataattat agattaattt
    18121 tgggatttca actgataagt accaaaagaa ataataagca aattaagtga aattatggca
    18181 caagattatg ttaaaaggac ggtaaataat gaattggata gagcttaaca atcaagtgat
    18241 aattaaaaat gaacaaggca agtaccaatt ggaaaaggat aaggaagcct taagtagtta
    18301 catagaagaa tttatacagc caaagttgag aaaatttaat aacctagaag aaagattgaa
    18361 gtatttaata gatgaaggtt attattcaaa agaagtaata aatcagtata gcatagatgt
    18421 tattaaaaat atatatgata tgattgataa acataagttt gaatttcaat catatatgag
    18481 tgcaaataag ttttatcaaa attatgcatt aaagagcaat gatggaaagg aaatattaga
    18541 aacatataat gataaagtgt taattgtagc tttgacacta ggaaatggtg ataaagattt
    18601 agctttaaac ttggcagata aaatagtaaa acaagaattt caaccagcta cacctacttt
    18661 tttaaatgct ggaagaaaaa gggctggaga aatggtatca tgttttttac tatctgtaga
    18721 agatagtaca gaaggtatat catatgctat atcttcatca aatcacttat caaagattgg
    18781 tggaggagtt gcactaaatt tatctaaact tagagcatct ggcgaatcta tcaaagatat
    18841 agaaggtgca gctggaggtg ttgttggagt agctaaaatg ttagaacaat catttagtta
    18901 ctttaatcaa atgggagcta gacaaggttc tggtgcggtg taccttactg tattccatcc
    18961 tgattttgaa ttgttgatgg atactaaaaa gataaatgca gatgaaaaaa taaggcttgc
    19021 tacattatca ttaggagcta taataccaga taaatttatg gagttggcag aaaaaaatga
    19081 agtagcatat gcattttatc ctcatactgt gtataagaaa tatggagttt ctcttgatga
    19141 gatagaaatg gatgagtggt atgataaatt ggttaatgat agtgatataa gaaaaaaaga
    19201 aataaatcct aggcagatgt tgacaaaaat agcacagatg caacaagaaa gtggatatcc
    19261 atatgtagtg tacatagata cagctaatag agaacataca ttaaaagatg ttggaattat
    19321 taaaatgtct aacttatgtt gtgagatatt tcaatatcaa acaccttcag aaatagaggg
    19381 gtatggagga aaaaatgaat ggggacaaga tataagttgt aacctaggtt ctttaaacat
    19441 agctaatgta atggataata aaactataga aagtacagtt gaaacagcaa taagagcact
    19501 gtcatttgta gcagatagca cagatataaa accagtgcca acggtaagca atagtaacag
    19561 caagtctcat tctataggat taggagctat gaatttacat ggatacttag taagagaaaa
    19621 tattttatat acttcagatg atgctataga tttttctaat gtattttttg caatggttag
    19681 atactattca attaaagctt ctatgaaaat agctatagaa agaaatcaaa cttttgaagg
    19741 atttgataaa tcagaatatg ttaagggaag aaatagcaag gttttatcaa aatactatga
    19801 acaatcttat ctacctaagt cagaaaaggt aagagcatta tttgaaggga tttatatacc
    19861 aacaaaagaa gactggacaa aattattgga tgaggtaaaa gaaaaaggga tatataatgc
    19921 atatttaatg gcagtagcac caactcaaag cattagttat gttcaaaatg ctacatcaag
    19981 tataatgcca attactgagc cagtagaagt aagaacatat ggagactcta ctacaatata
    20041 tccaatgcca tttttaacaa atgataatat gttgtattat caatcagcat acaggatgga
    20101 tatgagaaag gtcatagatt tagtggcaac agttcaaaac catgttgacc aaggtatatc
    20161 aacaacactg tttgttacag atgaaaagac aactagagat atagctagac attacatata
    20221 tgcctataaa aaaggtttga aaagtttata ttacacaaga acaaagatga caagagatac
    20281 tcatgagtgt ctagtgtgtt cagtataggg agagaaatta tgacatttaa tttacataaa
    20341 atacataatg cagttaactg gaatagagaa gaagatggat ttacacaagc tttttgggaa
    20401 caaaatgtaa aacaattttg gcttcctgaa gaaatatctg tttctaaaga cataaaggta
    20461 tggaatgaac taagtaataa agaaaaggaa ttatataaaa aagtattggg tggtttaaca
    20521 ttactggata caaaacaggg gaataatgga ataccatcta tgatgagttt aacagaaaac
    20581 ctacaaagaa aagctgtctt gtcttttatg gggactatgg aagaaataca tgcaaaaagt
    20641 tattcttcta tatttatgac tctactttct aatttagaaa tagatgaatt atttgaatgg
    20701 atagaaacag aaccaacatt acaaagaaag gctgatttag ttttagctca atacgaaaat
    20761 actacaaatc aagagggatt atacctatca atggttacaa gtgtattttt agagagtttc
    20821 ttgttttatt caggattctt ttatccattg tatctatctg gtcaaggaaa gatggtagct
    20881 agtggagaaa taatatcgct tattcttaga gatgagtctt tacatggtaa gtatatagga
    20941 ttgctagcac aagaaatata cgattcattt gataaaatgg ataaggagat gttagaagag
    21001 aagatgtatt ctatacttta tagccttatg gagaatgaga tagagtatac aaatgttata
    21061 tacagagaaa gtgggcttga aaaagaggtt gtgaactttt taaaatacaa tgcaaataga
    21121 gctttagaaa atctagggtt tgagaagcta tatactgtag atgctataaa tccaattgta
    21181 ttaaatggtc ttagcactga aaccaaaaca catgattttt tttcaactaa gggtaatgga
    21241 taccaaaaag gagtgtatga ggaattagag gatgaagatt ttataatata atttataatt
    21301 atgacacact gttgtcactt ttagcgtgat aatatcaatg tataaaatat atatggaggt
    21361 atttttatga aatatgaatg gagaaaaaga gataaagagt tatatttacc taaaaagaat
    21421 cctgtaatag ttgaaatacc agaacagaaa tttatcatgt taaaagggag tggagaccca
    21481 aattctaaag aatttactga agcaatagga gtactttatt ctttggcata tgcaataaaa
    21541 accatgccta aaaaaggaat tgtaccagaa gggtattttg actatacggt ttttccttta
    21601 gaaggtatat gggataaggg agaaaaaagt aaaggcttag atgtacttat taaagaagat
    21661 ttaatttaca ctataatgat acgtcagcca gattttgtta cacaagacct gttggataaa
    21721 gctactgata tagtaaagaa gaaaaaacct cataatcttc ttgaaaaagt tagatttggg
    21781 tctattaatg aagggctatg tgttcaaatg cttcatatag gttcatatga taatgaacca
    21841 gaaagctttg acattatgaa cgaattttgc aaaaaaaata aacttataag aaaatcttac
    21901 acacatcgtg aaatttatat aagtgatgct agaaaagtat cgcctgaatc attgaagacg
    21961 gttttacgtt ttgaagtaga aagggaagta taaatatttt gaataattta taagagttat
    22021 aaaaagtgaa gtctgttgag acttcatttt ttgatgcaat tttaaaatta aatttgatgt
    22081 ttcataatat tttaaaaact ttctactttt attagtgtga ataactgttg ctattaaata
    22141 ataatactaa ttatataagt attattatat aatatttata gattattata taaatagatt
    22201 attagtaaga tgtatgcatt attaattaaa ataaattaca tatactattt aatatttcat
    22261 ttaaaatatt aaaaaatgta gataaaaatt gttttattaa gaaaaaatta atatattgac
    22321 aaaatttaag tgctcattta aactattaat tgataataat attcaatatt aaataatagg
    22381 aggcgttatg aataaatatc ttctaataaa tccagtggca gaaagaatgt atggagaaaa
    22441 gtttcacctt attaaagcaa atttgataaa aaaggggtac ataataacag aatgtgaacc
    22501 acagttagaa tatgtaaaaa agcagtacaa agagtataca aaaaagactg aaaatacaat
    22561 gttggattgt agatgtccag aaagtataag tcttttaaaa agaaataatt tgataaataa
    22621 ttttgaagta cctatgatag aaccaatatt agtaaggact tgtagggttt tatacgataa
    22681 atatataaaa agtaaagatg atatgttggt agtaacctgt ccatgtacac aattaagaga
    22741 ctttgcaagt gaaaagttta aggataaggg taatgctatg ttttatacat ggaaagagtt
    22801 tgctgaacaa gaaggtgtaa aatcacttgg aaaaatagat gaatcaccaa ttccattagg
    22861 atatttcaaa gatacttttg aaaaagttct tgaagtatca agtgaagacg aaatattaag
    22921 tgaaatacaa aaaataagaa atggttttga agataagtat gacatagtag aaatgtttta
    22981 ttgtaaagat ggttgtaata atggagatgg tttgtaaatg gtcaataaat ttaataataa
    23041 gtttttaagg ctagtaaaga gtgttctgat attttttata attcttttat tatggaaaat
    23101 tacaaattat cttggtattt ggagtgatta tatattacca tcacctgaaa aggtatatag
    23161 tacattttta aatatgataa gtgatggttc aatatttatt aatgtatatg caagtatgaa
    23221 aagagttttg ataggttttg caataagtac tgctataggt gtgcctttag ggatattctt
    23281 tggaatttat agtggagtat acgaatactt taaatctcta ataaattttt taaggaatac
    23341 acctcccctt gctttaatac caatgcttat attatggttt ggtataggag aggaatcaaa
    23401 aattataatt atagttttag catctttttt cccaatcttt acaagcacat tgaaagggat
    23461 taaaaattgt gactcaaaac tgattgaagt gggtagagta tttgagtttt caaagcttca
    23521 gataatattt aaaataataa taccaaatgc cattcttgat attgctgttg ggttaaagtt
    23581 agctcttgga tatagcttta gagcaattat tggggcagaa cttgtagcag cttcatctgg
    23641 tcttggatac ttgatatctg atggaaaaga aatgtctaga acagatgttg ttattgtagg
    23701 gataatcgta attggacttt taggaataat tacagattac atattttcga ttattgtgaa
    23761 gaaggtaagt aagggaaaga tggtggaagc atatgaataa tttagtatgt gactctggat
    23821 atataattag aaatttatat aaaagttata aagttgacaa taaagaacat gtagttctta
    23881 gtgatatctc tttaaacata gatagaaacc atataacagt aattttaggt gaaagtggtt
    23941 gtggaaagac taccttgctt agagcaattg catcacttga aggtataaat agtggtgaaa
    24001 ttaggtttat taatgataat aaagagtata gacctaatgt tggttttgtt tttcaggaga
    24061 gtagattaat gccttggtta aacgtaagtg aaaatatagc ttttcataat caaagaaaaa
    24121 atactatttt aaacaaggtg cttagaaaat ttaaacacaa taaaagaatt aggagtaaaa
    24181 ttataaatga taattttaga agcttagaaa caaatgataa tttgaacaaa tcaaataaat
    24241 ataataggaa aagtaatatt gatgtagaaa aatatcttaa gatgatgaac ttgcagaaat
    24301 ttaaaaactc ttatccaaat gaattgtcag gaggtatggc gcagagagta agcatagcta
    24361 gagctctttc tttcaatcca gatatgttgc ttatggacga gcctttttcg gcattagatt
    24421 attttacaag gattgatatg caaaatgaag ttataagaat acataagtca actaataaag
    24481 gcgtgatatt tgtaactcat gatattgatg aagcgttaaa gataggcaag aaaatagttg
    24541 ttttcacaga cgaaagaaaa gtaaaagaat ttaatataga agatggttat aatagagatt
    24601 taacttcaga ttactatatt aaattaaaaa aagatatttt aatgacaatg aagtcaaata
    24661 aaggcattta aagaaattaa taatatttta aggagtaaag taaatgaaat taaaaaagct
    24721 attgataggt ataacgatat taacacttgc aacagcatct ttagctggat gtgctaaaaa
    24781 aaataatgga gaaaaacttt cagaaataaa tttaacttat gtaaaatcac cattaaatgt
    24841 tccttctata attcagaaac aggatgattt atttggaaag gaatttaaaa aggataatat
    24901 aaaagttaac ttccacgaaa ttactacagg tccagagcaa acacaagctt tagctgctgg
    24961 agaaatagac ttcttacatg cactaggtgg tacatcagct ttaatagctg catctaatgg
    25021 agtagagtta aagatattaa acacttatag caggtcacca aaaggattta tgattttgac
    25081 taataataat tcaataaaat cagcagcaga tttagtaggt aagaaggtgg caggcccaaa
    25141 agggactata ttacatcagg tattgatttc agcacttgat aaagaaggat taagtatgga
    25201 tgatgtggaa tttgttaata tgggtattcc agaggcatca gcagctcttt ctgatggcag
    25261 cgtagatgct gcattgattg ctgggccagc agctcttaaa gctatgaagt caggtagtaa
    25321 acttgtagca aatggtgaag gtttagtaga tggtataata gtaactgctg ttagcacaga
    25381 ctttgcagag aaacatccag aaatagttga aagatttatg aaggttgaaa aggaaacttt
    25441 agaatatgta aataataatt ttgatgaagc tatggaaaaa gtagctaaag aagttgattt
    25501 aagtcttgaa gaaactaaag aattatatgc ttggtatgat tttagtttag atataacaga
    25561 taaagacata tcttcgcttg aagatacaca agacttctta ataaaaaata agttacaaga
    25621 gaaaaaagtg aacataaaag aattaatcta taatgctaag tagaaactat gataaaataa
    25681 tactaagtaa gaaatataat aaaagtttaa gttaccttat taaggtaact ttttctattt
    25741 tgaattagta atttcaattt attttagaat gattagttga taataatgat taattaatca
    25801 taaaaagata cttttttata gagttttatt aagatgtcta ataatatggt tatacttatg
    25861 aagtcagtaa ttgtgaagtt agtaatatga ttaaatttat atattgtaaa aagattataa
    25921 tatattaaaa ttggactgtt atatgatgag taaataggta tattaggagg aattatatga
    25981 gttataaagt atatattgtg gaagatgaca tatcaataag tacattactt aaagattata
    26041 ttgagaaata tggatttgaa gtgattgttg aagagaactt tgatgatata atgattacat
    26101 ttgaaagttt taagccacac ttagttctat tagatgtaaa tttacctaaa tttgatggtt
    26161 tctactggtg tagatgtatt aggcaacagt cgaatttacc tataattttt atatcagcta
    26221 gagatggtaa tgtagaccaa attagagctt tagaaagtgg agcagatgat tatataacaa
    26281 aaccttttta ctatgatgta gtaatggcaa aaataaagag tcatataaga agggtatatg
    26341 gtgaatattc acctaaaata gacgaaagaa tagtggagct agatggatta aaattttatc
    26401 cagaaaggtt agaactagaa ttaaatggtg agaatctaat tataactaaa aaagaaggta
    26461 tactgattga atgtcttatc aagagatacc caaaagtagt aagtagagat tatcttttag
    26521 aaaagatatg ggatgatatt gagtttgtag aggaaaatac tttaaatgta aatgttagca
    26581 ggataaggaa aagatttgag gaattaggta tagataatgc tgttcagact gtccgagggt
    26641 taggatataa attaaataag aattggtaag gagaaagtat gaagttattt ttaaaagatt
    26701 ataaaggtta tatttttata tattatatag gcatagttat gacaattatt tactgtaatc
    26761 ttatgaagtt tattagtttt ggagagagtt tatatattct tttatttagt tcttttatat
    26821 tgttatgttt tttaatatat aagtattata aaaatagaga agtatataat ctgtttgaaa
    26881 aaggagtaca ttctttggag gagtcatttt tgtatttagg aagctcattc ttggggagaa
    26941 atatatcagt tattttaaaa caacaacata aattgtatca atcaatgata caagagcata
    27001 aaaagataca tagtgaacat ttaactttca tcaaccaatg gattcatcag atgaaaacac
    27061 caatatcagt tataagcctt cagctccaag attatgaggg agaagaagta actgaaaata
    27121 taagaaaaga aatagataga ttgaataaag ggttagatat ggctatgcat tttgcaaggg
    27181 cagataattt tcagaaagac tttatagttg aaaaagccaa attaaatgaa attgtatcta
    27241 gtattgtaaa tgaagaaaaa aggttattta taaaaagtgg aatgattcct aagattcaag
    27301 tagacaagtt tacttatgtt tatacagatg taaaatggat gaaatttata ataggtcaat
    27361 taattacaaa tggagtgaaa tattctaaag attatagtaa ctacctaact ataaaatctg
    27421 aagaaaataa taaatatgta attttaagta taatagatga aggaattgga attgtttcta
    27481 aagatttaaa aagggtattt gccccatttt ttacaggaga aaatggtcgt aagtttggag
    27541 aatctacagg tatgggtctt tatctagtaa aaaaggtatg tgatgattta ggtcataaag
    27601 taagtataaa atcagaagta ggaaaaggta cagaagtttc aatctacttt aataaaggtg
    27661 taaatagttt gataaagatg taaattatct gttacaatag ggtttttagt taattgtact
    27721 tttttattat acaatttatt aaatgaaata ttcatagtta aataatcaaa atttaagaga
    27781 gtttatttgc aaaccttaca tagatgtaag gtttgtgtaa tgtttgcaga tagttaaatt
    27841 ttctaatcta aattacaatt aatataagaa ataacataag agaaataaca taaaaaaaga
    27901 aattttgtaa aaaaggaagg atgtttattg atgagtttag atgttatttt tatattgaaa
    27961 tcagtcataa tagctatagt agaggggctt acagaattta ttccagtttc atctacaggt
    28021 catatgattt tggttggtaa tttaatagat tttaaggggc aatttgctga aatgtttgag
    28081 gttgtaatac aacttggggc tatattagca gtagtagtac tttattggaa aaagataaaa
    28141 gatagcgtta tagaattttt taaatttata tttactggtg gaaaagaagg aaagattggt
    28201 tttagatttg gaatgaatgt aattataggt tgtataccat ttgcgattat aggagttcta
    28261 ttttacgata atataaaatc attgtttaat ttacaatctg taattatagg atttatagtg
    28321 ggtgggattt tattattagt agtagaaact ttgtttagaa aaaagaatca ttccacagat
    28381 aatatagata aaattactcc aatacaagct ttaaaggttg ggacattaca agttttgtct
    28441 gcatggccag gtatgtcaag gagtgcatct actattatgg gtggatggat agcaggactt
    28501 aattctccta cagcagctga gttttctttc tttttagcag taccagctat ggtggcttct
    28561 tcaggaaaag atttatttga atttgattat tcaataatga caccaacatt gtggattgct
    28621 ttggtagttg ggtttatagt agcttttatt gtatcaataa tagttatgga aaaattcgtg
    28681 aattttttaa agaaaaaacc aatgagagtg tttgctgtat acagaataat catgggtgta
    28741 gtacttgcag tattagcttt tactaacata attagtgtat agaaaaaata gatttattat
    28801 ctataaaaat taaactaaga taaattataa ttaacaatta tatatatgtt atttcaaaaa
    28861 ttaaattagg aaggatagtt aaataatgaa aattttaact gtaaataatt taagtaaagt
    28921 atatggtaaa aaaataattt tcaacgcatt aaatgacata aattttagta tagaagatgg
    28981 agaatttgta ggaattatgg gtccatctgg tagtggaaaa actactcttt taaatatgat
    29041 ttctactatt gacaagccaa ctacaggaac tatggaatta aagggcaaga atcctttatt
    29101 gttaagaggt gaggaacttg cattatttag aagaagagag ttaggttttg tatttcagga
    29161 ttttaattta ttggatacat taactatagg agaaaatata gtattgccat tgacattaga
    29221 taaggtcagt gtaaaagagc aagatgagag attgaatgaa gtatctacaa tattaggtat
    29281 aaaagattta ttaggaaaaa gaacttttga agtatcaggt ggtcaagctc agaggactgc
    29341 tattgctaga gcattgatta acaatccatc aatactttta gcagatgaac caacaggtaa
    29401 tttagattca aagtcatcaa aggttgtaat ggagctattt caaaagataa ataaagaaaa
    29461 taaagttact actatgatgg taactcatga cccattagca gcgagctact gtagtagaat
    29521 cttattcata aaagatgggt ctatatataa tgaaatatat aagggtagta gtagagagca
    29581 gttttatcaa gagataatgg atgtacttac attattggga ggcgataact aatgaatata
    29641 aaacagtttg ctattaacaa tatttcaaga aatattaaag cgtatttagg atacttagca
    29701 agtattgtta tatcatcttc actgctattt tcttttatta tgtttacaag tcatccagat
    29761 ttagatgtat ctcaatttcc tgattattta aaagaaggtc tcaagatgag taagataatt
    29821 gcatatctat tcttattctt ctttgtgttt tattcagtta gtgtgtttct taaaagtaga
    29881 tataaagaat ttggaatact ttatattaca ggtatatcaa agcgccaagt aatgaaatta
    29941 atctttatag aaaatatgat aataaatata gtatcttctg taattggaat aataataggc
    30001 ttaatctttg caaagatatt tttagtggtt atgtctactt ttttagaatt atcaccatta
    30061 aaattctata taccatttaa tgcgatattg tctactttaa tctattttat gattttagct
    30121 gtattaacat caatatttac ttcttttata gttaaagaaa atcaggtgtt gaagttactt
    30181 aaaggtacaa aaactccaaa accagagcct aaaacatcac taatgcttgc tatattaagt
    30241 atttgtttgt ttataatagc gtattataat gctattacct caaccgacca atatacaaca
    30301 tatctgcgct tagttccagt tacatccctt actatagtag caacttactt gttcttttct
    30361 caatttagtg tattttttat gaagaagcta aaattaaaca agagatttta tagaaaaaat
    30421 acaaatatgc tttggatttc gaacttgatt tataaggtaa aggataatgc tagaatattt
    30481 ttcttgataa ctataacttc tgcagtggca tttactgcta taggtacagt ttattccttt
    30541 tggaaagatg tagagcgtca aattaattta atatatccta acacaatata ttattcaaca
    30601 atgacattgc ataatgaccc taaaaagcct gatagcaaag aaaaagacaa ggaaagaatg
    30661 atgtttatag aaaataatct aaaaaaagag aatgtagact atacaaggat taatggtgaa
    30721 tttaaaactg tatttcccaa gaaaagtgat tttaatgtca gaattatgaa agaatctaag
    30781 tacttagaaa ttacagaaac tataggggtt aagcctataa gttttgaaga taatgaatgt
    30841 atatcattat tatcatcaag tttaccagga aataagaacg tcaaagaaaa tattattgta
    30901 gggaatagag gtttaaaggt ggcaaaacaa gtggaagaat gtgttatgcc agcgtattac
    30961 aaaaatatgt atgtggtaaa ggataatttt tatgatagta tagaatcaaa atttatagtg
    31021 gatagattta ctgcatttga agtagaggat tcaagtaaaa ctatagagat atgcagagaa
    31081 tttgaaaata aatttaataa tgagtcaggg atgcaaccat atttgttttt ctctaaagaa
    31141 ttaatgatag gaactgggaa attaatgtat tctacattta tatttttagc aatatttata
    31201 ggtttaattt tctttgttac tacaagtagt tttttatata ataaatttta tatggattgc
    31261 caagttgata agaaaaagta tgaacagtta aataaattgg gtatgacata taatgagatt
    31321 aaaaaagctt caacaattga gattggaata gtatttttgt ttccttatgt agtggctgtt
    31381 atacattcaa tatttgcact tactgcactg aaaaattctt ttgatataga agtagcatca
    31441 tcagctgttt tggtaatggg aagcttgttc ttggttcaaa tagtttattt tttaattgta
    31501 agaaataatt atttaaaaga gataaggtta aatttagtca atttttagtt actattatta
    31561 aatgataata aaaagggttt agaattaatt aatattactg gaattataga tgtttatttt
    31621 ttcagtaata ttttttattg tattaactat taaaattttg tacaaatata aaatataata
    31681 tggtaaatgt caaaattata aaaaaattct acttatataa aatataaaga ggttatatgt
    31741 acatatgtgt agaaataaat aataaaaata tcaattaatg ttattagggg gggttactca
    31801 ttagtaaatt agctaataca cttagaatga tgatattaat tcaaagcaga ggcaaaatga
    31861 aatgcaaaga attatcagaa gagttggaag taagtgaaag gcaaataaaa agttacaaga
    31921 tgtatttaga gcaagctggt atatttataa attcaacacc tgggatttat ggtggttatg
    31981 aaatagataa atgtaatagc atctcattaa ttaaattatt agatagtgaa gtttcaattc
    32041 tagatatgat aaattcacag ttagagtata ataatgatat ttacaaaaat gaatttaata
    32101 atattgtaga aaaaataaaa gcagtattaa atacaggaga aaaaagcgat acatatatgg
    32161 attattttac agttcaagca caacgtaatt gtgattatga gtctgaaaaa aataagtgta
    32221 atgaaataat tagagcatat accacaaaac acaaattttg gatagaatat tattcattaa
    32281 attcaggaaa tagtgaaaga attgttcatc catatggttt atttaattat aaatcagata
    32341 cttatatggt agctttttgt gaaaaaagat ttaagtttat agactttaaa ttatgtagga
    32401 ttaaagatta taaggtttta gaagaaaaat ataatgtaga taaaagtttt agttgggatg
    32461 aatatagtaa aaatagtata ggaatttata agggagaaga aattaatgtt gttataaaaa
    32521 ttagtcatcc attttcaaca attataaagg agaaagtttg ggttaataat caacaaatca
    32581 tagaatatga tgataaatct ataatgttta aagctaaaat gagaggttat gaggagataa
    32641 aaagttggat tttaagtatg ggagcttatg ttgaggttgt agaaccagat agacttagaa
    32701 atgatatatt gttagagatt gaaaaaatga aaaaaattta ctgatttttt caaataattt
    32761 atacttggac caatatactt cacggtctag tgttaatata ttaattgaga gaggtgatat
    32821 tatgaaaatg aaaattgaat ttagtacagg ttgtatacca atatcttata attcgctctt
    32881 tatgagtatt ataaaagaag caataagaaa atctaatgaa gattactata aaaacatgta
    32941 ctattataaa gaaaagaata ataaaaaaac taaaaatttt acattttcgg tttatataaa
    33001 aaagtatagt attgaaggtg acaattttat tatagaagat aaagtaatat taaatataag
    33061 tactccagat ttagaattgg gttttcatat atacaatggg ttaatgactt ctaaaaaatg
    33121 tttatataaa gactacgaac ttacaagaat tagaatcgat ttatcaagag aaaagaaggt
    33181 aacagaagaa agggtactat ttaatgcatt gtctccaatc tgtgtaaagt ctaaagaagg
    33241 aaaattcttg gagataactg atgatagata catagaagaa ctaaactata taactaatga
    33301 agtggttaaa aattataggg ggaatggatt aaaaagaaag ttagaatttg aaaatatagg
    33361 tctcaaaaag gtagtagtta aagagagttt aagagaattt aaaaaaataa caggtaaaga
    33421 gtatcaatat attaatggtt acaaaggtaa atttatattg aaaggtgata ttgatgattt
    33481 aaatcttatc tacaatctag gaattggttt tagacgtgct cagggatttg gagatgtaga
    33541 tattttagaa tggaggtgag ataaatgaag ttaagagttt atagaggaga ttggtttttt
    33601 aatatgggta tagttggatt tttaaatata ataaagaaag cagataaaca agctgaaata
    33661 tttattatgg aagattatat cgaatttgat agcttatttc ttgaaaactt tcatgagtat
    33721 tattttaatt actttatgga tgaatatgat gtatcaaaaa gaattaaaaa aaatattgat
    33781 tacagtataa attttattaa atctaagcct gatagaatca aagatggaat taagaaaatt
    33841 aaagatagtg tgaaacaaca aaatgataag attaaaaaat ttgatgaaga aaaatttaac
    33901 atgataaaag agaagctaga tagtatgtct aagataaaaa gttatgatga atttgatagt
    33961 ttagaagctt tagtagatga gactatagat atatttaaaa taaaatcaat aaatgacagg
    34021 cttacagcaa atctttataa atatgtagtt caagataatt attttggaca agttagtttt
    34081 tttaatgttg ctaaggcgaa attagattta gatggattaa aacaggtaat gtttaatgat
    34141 tatttaagac aaattatata ttttggagag ttagaagatt tattagaaga gaatgactat
    34201 gacaagttga aaaactattt aaatgataga ttaaatagta tagctaagga tataaatgaa
    34261 aaaaaagtta gtaagtctag tataaatact atagaaaaaa ttatgaagga aataaataaa
    34321 aactttataa agaaaaataa aagcatagaa gatataaaaa agtatatgga tagtttagaa
    34381 gtatgtgaaa tgtgtggttt atataaggga atattagatg aatactcaga aagtaatttt
    34441 gctccacttg gagtaagcac caataatgct aggaatatgt tttggaatca ggcgtatgta
    34501 ccatcaatat gtgatatttg taagctaata ttgttctgta ctccagcagg ggctacttat
    34561 acaagaaaga attatttaat taatgaagaa aatgagtttt atttatttgt taacatggat
    34621 acttgtataa atgagctttt tgaaagaaat aatagtttaa aagctcaaaa aagtgagtat
    34681 tcagattcca aagatgaaaa tccttttaat cagcttataa aaagtatagt agaagaaaat
    34741 acattaaaaa gtgaatggca acttcgtaat atactttttg tggaatttaa agcaagtata
    34801 gattctaaaa aatgtaaaat aaattatttt aatattccaa cttatttagc aaagtttttt
    34861 gtaaaagaaa ataaaaaaat acaaagtata tatgattata aacttaaatc aaatgtgtta
    34921 gatttaattt taaaaagtaa agacttaaaa catttaatta ataacacttt aaggaatata
    34981 gttaagagta atttagaaag caataataaa tcaaacatat cagggttatc ttgtttcaat
    35041 gttgttcaat taagaacaat attaaataac tataagagag gagtgtataa aatggactat
    35101 aaaaagttga gaaagattta tgagtcaggt tgtgaaatac atgattatta tattgataag
    35161 aatgctaaaa ataaaataga aggaataaca tataaaattt taaatttaat taaggttggt
    35221 aataaaaatg attttatgca aacaatttta agaatttttt caccaagtga aaaaacacca
    35281 ccagaagaaa ttttacagat atttgttgaa gaagatttag attttgaatc tgtagggtat
    35341 gcatttgtaa caggattaat ttctggaaaa tatgaaggaa aagataaact accaaacgaa
    35401 aaggaggaaa aataagatga aaagagggtt aacatttaca gtaatagcac aggctcaaag
    35461 tttaaactat ggggaaggga taggtaatat atcagaatta aaaaaattaa gtagagatga
    35521 tggaaatata tatacatttg catctcgcca gtgtttaaga tatgacatag tacgattagc
    35581 agcagaattg tttaattgga atttacaggt tgtcgataaa gaaaaaggca ctattcaatt
    35641 taaagacaat ataagtataa gagaatcaga agaaatggat ttatttggat atatgaaaac
    35701 aaataaaaaa gatgagaaag ataaaaaagg aggttctttg actagagaag ctactgtaag
    35761 attaagtaat gcaatttccc ttgagccata tagaagtgac atggactttt taaataataa
    35821 aggattagca gatagaatag gagaacatcc taatcttgct aacatagagc aacatttaag
    35881 ttattataca tatacagtga ctatagattt atctaaaata ggtaaggatg gagatataga
    35941 acttgataat aaagagaaat gtagaagagt agtagaattt ttagaaataa taaaagtatt
    36001 gaatagaaat attagaggta gacaagaaaa tttatctcct ctatttgtag taggaggagt
    36061 atatgaaata gcaaatccat ttttcttggg tagaattaaa ttaaaaggag ataaaaatgg
    36121 atttaaaata aataagcaag ctatagaaga agtaattcaa ggtacttttt taggaaaaga
    36181 tttaaaagaa tttacctatg ttggaatggt agatggggta tttataaata aagaagaatt
    36241 taagggattg tttgaagata attttttaag tgtagataaa ttttttggac aactagttaa
    36301 agaagtaaag gaatattatg gggtgaatta atatgaaggt tttgaaatta gatttatttc
    36361 aagaaactgc atgctacaaa aaaccatttg ctatgaagat tacagagaca tatccactgc
    36421 ctccatattc tacaataaat ggattaattc acaaaatatt aaatgctaca gaatatatac
    36481 catttaatat aagtgtacag ggaacatatg aaagtatatt taataattac cagacaacat
    36541 atttttataa aaaagatagt attacatcta tgccaatgaa tagtcatatg ctattgaatg
    36601 taaatttgat aattcatata ggtggggatt ttgatttatt ggaagaaata tatgatagtt
    36661 tattaaatta tgacgagcac ttatcattag gtagaaaaga agatttagta agaattaatg
    36721 atattagatt tgtagaagtc aataaatttg aagtagatga ggatatacaa ttagatgatt
    36781 atgaaaatta taagttatca gaatgtaata taagaatgcc tatatatata ccaaaaacaa
    36841 gtttacaaga tacagatata gatggtataa gttacagact caataattac tatgataata
    36901 atgctgtgga agataaaaaa agggtttgga ataaggtaga tagttattat gttgaggatg
    36961 gaaatactat atcaattgga gatatattat tagataatga aggagattta ttatatttta
    37021 ataatataaa ttaggaggga gattatgtta tatgctaaat ctaatccagt ggaaacactt
    37081 agagaacata ctgatgaact tttaaaacaa atgaatgtat taagagaaag ttatggaaaa
    37141 aatataaata gcttagattt tctagaagag gagatatttt ggcaattact tgactttgtt
    37201 atagaatttc atgatattgg aaaagcattt tcaccgtttc aagagttaat aaaaagcaga
    37261 atggatacta aaataaatga acctaaaata gtaacttatt tagaaaataa tgtaggccat
    37321 aattatcttt ctcctgcatt tattgattat agttatatag acaggaaaaa aaataaagaa
    37381 cttagagcag tattaaatca agttattgta tatcatcatg aaagagatat ctttatagat
    37441 aaagatttta aaaatttaat acaaaaaata ttagatgaag atttaataaa taaagtttat
    37501 gaactacagc atgaatttaa agttagatat ccaataaaaa cagaaaaatt gagtaaagta
    37561 tatctacaaa gtgtagaaaa aagaatagat aaaaatcaca agtactataa tttatatatt
    37621 atgttaaaag gaatactaca tagactagac catagtgctt ctgctcatga agttgtggaa
    37681 tgcaataatg ttataaatat aggagaacag acagaaaatt atttaatgaa agagtttggt
    37741 agtttgagag aggctcaatc atttgctaaa tctaatagaa ataagaatat aatactgatt
    37801 gcatctacag gaatgggaaa aacagaaact gccttaattt ggatagataa agacaaggca
    37861 ttttttacac ttccattaag agtaagtata aatgcattat ttgatagagc aaaaaatata
    37921 gttggtgttg gagaatcaaa tgatacattc ttaggtttgt tacattcaac agcaatagat
    37981 tatctagaag aaagtaatca agaaaattca agtgagattg tagatttagc aaagttatta
    38041 tcttgcaaac tgacattttc tacaatagac caaatattta aatttccttt tttgtataga
    38101 gggtatgaaa aagtgtactc aacattagca tattcaaaag tagtaataga tgagatacaa
    38161 gcatattcac cagaaatagc agctgtattg gtaaaaggta tagaaatgat acataagata
    38221 ggtggcagat ttatgataat gacagctact atgccaacca tatatataga tgaattaaaa
    38281 aaaagaggtg taatgaatag taacttagct gaattgacat gtaatacaga aaaaataaga
    38341 cattgtgttt ctattgtaga aaattcaata gatgaaaact taggtaaaat aatacaaagt
    38401 ggaatgaata aaaaagttct tgtaatagta aatacagtta aaagtgctgt agaaaaatat
    38461 gagttaatag aagaaatagt taaaccaaaa ggaatagatg tcaacttaaa tcttttacat
    38521 tctatgtata tacaagaaga tagagcaaaa ttagaaaaat atataaaaga atttgccaat
    38581 agtgatagaa atggaatttg gattacaaca caattagtag aggcatctct tgatattgat
    38641 tttgatgagc ttcatacaga aaattcaaca cttgacagtc tatttcaacg atttggtaga
    38701 tgttatagaa gtcgtgagta tgaagaaaat agcccaaata tatttatata tacagaaaaa
    38761 gctacaggaa taggtagtat ttatgataag gatattgtag aaaaaggact tgagctactg
    38821 aaaactttta taaatggaaa agaatgtgtg aagatgaaag aaaaatataa ggtagaaatg
    38881 gttaagatac tatactctaa agaaagtcta gaaggtacag cttttgaaaa gagatttacc
    38941 agtgctttaa atattttaga tactataacg ccatatagtt taggttctaa agatgcacaa
    39001 aatatactta gaaatattga aggatataca gtaattccag aggatatata taataaaatt
    39061 gaagaaactt taatcaaaga ttatgaagag ttgggacaag agcttataaa tgcatatagt
    39121 aatgatgata gacaattgat aaataaaata aagtctaaga gaaaaaaagc aagaagagag
    39181 ataattaaaa aaactgtaaa tttaccaata tataaagcta aacaaaatgt aattgacata
    39241 caggtgaaag gtcttgaaga tttaaaaata cttttatata aatatgatat atacgaaaat
    39301 aagcatacaa aacagatatt tggtaaaggt gtgcttataa gtaatgaaat agaccaattt
    39361 atttgatttt tatattaaca atgtggctaa aatttactaa agaaggtgat tttgtgccaa
    39421 tagatttaga agtattaaaa gttcaaggtg taaaaataaa ttattattat gtatgtaaaa
    39481 gaaaactatg gctattttct aaaggaataa ccatggaaca aaatagtagt agagtattgt
    39541 ctgggaagat agtacatgaa gattcatata aaagaatgaa aaataaagaa atattgattg
    39601 atgatatctt gaagctagat ataataaagg atgagtatgt aaaagaagta aaaataagtt
    39661 caaagatgaa agaagcagat gaaatgcagt tatactatta cttatactat ttaaagaata
    39721 gtatgggtat agagaaaaaa ggtactataa attatgttaa ggaaaagaaa caagaagaat
    39781 tgatattgac agaagaaaaa gaaagaatta ttgaaaacac acttataaat ataaatgaaa
    39841 taaatatgca gttatctcct cccaaaattg agaaaaaaca ttattgtaca aaatgtgctt
    39901 attatgaatt ttgttttgtg aaggaggagt aacaatgtat aaagactatt atattataag
    39961 taatggggat ataaaaagga aagaaaatac gatttactat tacaatttag aaggtgagaa
    40021 aaaaatatta ccagtagaac aagttgatga tattcatata tatggagaag tagatttaaa
    40081 tacaaaactt ataaattaca taagcaaata tggggtgatg cttcatttct acaattacta
    40141 tggatattat tcaggtacat attatccaag gaagaaaaat gtatcaggat tcagtttagt
    40201 atgtcaatca gcctgctatt tagataaaaa tgaaagatta tacttagcaa aatcctttgt
    40261 ggaaagtgca tcataccata ttttaagaaa tatgagatat tacaaagttg atgaaagttt
    40321 aattgatact ataaaggatg aactaaatga tttgaatgat gtcaaagaaa tatcagaact
    40381 tatgggtaaa gaaggcagaa taagaaaaac atactataaa tcatttaatc aaatattaaa
    40441 agatgatttt gaattttata aaagagaaaa aagacctccc aaagacccag taaatgctct
    40501 tatgtctttt ggaaatagca taatgtatac gaatgtttta agtgagatat ataagacaca
    40561 acttgaccct acaataagct ttttacatga accatcaaca aagagatttt ctttaagttt
    40621 agatattgca gaaattttta agccaattat aattgacaca ataactttta gcctgattaa
    40681 taataaaaga attaataaga atgattttga atatcaagac aaaatttgtt atttaagtga
    40741 aaaaggaaag aagaaatttt tgcaagaatt tgaaaataag atgcagacaa ctataaaaca
    40801 tagaaatcta aatagaaaag tttcatatag aatgtttata aggttagagt gttataaatt
    40861 aattaaacat tttataaatg atgaaaggta caaagtttta aaggtgtggt ggtaagtatg
    40921 tttgttattg ttacttatga tattgttgaa gcaaggtcgt taaatagaat tagaaggata
    40981 cttagaaaat atttgacttg gacgcaaaat tctgtttttg aaggcaatat tactgaagga
    41041 aagttacata aatgtatttc tgaaatagaa aatattattg ataatagcga ggattcaatc
    41101 tatgtttatg agataaaaaa tcctaattca attaaaaaga aatgttatgg gattgataag
    41161 tattctgatg aaatgtttat ataggtttgc agtgagcgat atttatgcta aaataggtgt
    41221 taacagttgg aatataaggg attgaaggtg tatgataatt gttatcaatt gcactactgc
    41281 tcgctcactg caaattttga tgtttttatt gaattataat tgcttgattg aagtgttttc
    41341 aatgtattca aatataccta ttttggggtt ttatattaac taagtggtat gtaaataatt
    41401 ttttcggata tttaataatt tattttaaat ttagttttat attaactaag tggtatgtaa
    41461 atctcaacag tatctacaag aacttctata tcttctaagt tttatattaa ctaagtggta
    41521 tgtaaattaa tttagggtaa cttttatatt attttcttct atcgttttat attaactaag
    41581 tggtatgtaa atttggttga tatagatatt agtcctatta tttctgcttg ttttatatta
    41641 actaagtggt atgtaaataa attagataat ttataagtca ataaaataag tccaagtttt
    41701 atattaacta agtggtatgt aaataaattt gtaatattat ataatcatcc taaaacaaaa
    41761 ggttttatat taactaagtg gtatgtaaat tcgaatgcta taattttaat atataaaata
    41821 aatggagttt tatattaact aagtggtatg taaatatgga ttttagtcgt ttttaaattt
    41881 ttctcgtaat agttttatat taactaagtg gtatgtaaat accattcatt tatattttta
    41941 tctgtatctc ttagccatgt tttatattaa ctaagtggta tgtaaattaa gttttcttaa
    42001 aaaagtactt gctttaaata tgttttatat taactaagtg gtatgtaaat tttattttct
    42061 ctttattagt cattagtttt tgccttcgtt ttatattaac taagtggtat gtaaatatga
    42121 agaatttgcg aaactaataa gaaaagatat tgagttttat attaactaag tggtatgtaa
    42181 atctttcttt atcaaagtaa gctaaattta gttcctccgt tttatattaa ctaagtggta
    42241 tgtaaatcgt cattactttt tattgcttcc attaaatttg ttcgttttat attaactaag
    42301 tggtatgtaa atatcgcaag ataggctaaa tctatcaagg agagttgagt tttatattaa
    42361 ctaagtggta tgtaaatgac tctgccatcc ctgtaacaaa cagtcttacc gcaagtttta
    42421 tattaactaa gtggtatgta aataaatatt ttacgattag ttgatgctat aattctatta
    42481 gttttatatt aactaagtgg tatgtaaatt cttttgattt agcgttgttt gtccggaatt
    42541 ttaaagtttt atattaacta agtggtatgt aaattcgggc tagaatgtat tttaaaccct
    42601 agttcattga agttttatat taactaagtg gtatgtaaat tttctaatat cgctatttaa
    42661 actcaatgct tcatttgttt tatattaact aagtggtatg taaattcaga acaaattaaa
    42721 tatgtttcgt tttccatgta aggttttata ttaactaagt ggtatgtaaa tcttgataat
    42781 atttcagcat atgatactga agatggtttt atattaacta agtggtatgt aaatttgcca
    42841 atgtaacatc tttagatatt acagtatcat tgttttatat taactaagtg gtagataagt
    42901 agaatattag caatatcaac gagtatttag aaacttattt tatattaata aagcagtata
    42961 taaaattgta gaatcaacaa tagcatatac taaaacatcc accttatatt aactaaatca
    43021 tatctaaata aaataaactt acctataaac attataaaat caatacaaaa atgaggtgaa
    43081 ataaaattta tgataaagaa attaaacaat aaagatataa ataaaatcat ggaaatatgg
    43141 gaaaaaagta caatcaaagc acatgacttt ataagtaaag aatactggca aaataactat
    43201 aatactgtta aaaacgaata tatacctata tcagatacat ttgtatatga tgatggagat
    43261 gaaataaaag gatttataag cataatagat aaaagcttta taggagcttt atttataaag
    43321 cccaaatacc aaaatctagg tatcggaggt aaacttttag attatgcaac taaaaaatat
    43381 aaaagtctaa gcttagcagt atataaagat aataaaaaag cagttgtgtt ttataataaa
    43441 aaaggtttta atatagtaaa agaacaagta aatgaagatt caggatttaa agagtacata
    43501 atggaatata gtaaataata tgattacatt ctattaaaag gataattaat ttatttgcca
    43561 tttgattttt taagagtaat ttttaaaata gactatatga agataaagat atttattata
    43621 gatgtaagtt aatcagaatt gatatagcta caaaagagga aaataaaatt gtatggtgta
    43681 tttcagattt aacaaattta ctatttaaag ataataagtt tgatataata ttaaagatac
    43741 tttcacctat aaactatagg gtatttgaaa gaattttaaa taattatgga atgctaataa
    43801 aggtttgtta aaatttaata attattagat tatagaaaat tgccatgaaa ttagttcatc
    43861 aagaactagt ttcatagcaa ttttaattct tataagtgta tttattttta cagatttaaa
    43921 aaacttatac agtatctaat aatgtaatct tattacttta gcctataaat atttaaatct
    43981 taaaatgttc tctttgagat aagtttattt cagagtcaca atttccacaa cagtaagatg
    44041 ttgagacatc tttaaatttt tcagtattct catcaaagtt catttttttc tttgcaataa
    44101 ttcctacctt agttaaatta gaaccacaat tattacagtt ttgaattttg cataggttat
    44161 atagtttatc aagtgtaata tcttctaata attcatcaca attacaacat ctgatagaag
    44221 aaacttcact acacatttca ataggttcag cttctttaga agaaaattta tatgttatgt
    44281 catttgatac tttataacct gtatcaaata tattagaatt acaacttgga cataagtact
    44341 ttggaagata tatatctttt ttttcaaaag tatcactact aatattacag tttaattcat
    44401 ctaaaattgg tctttcatct aggttattac ctttatttaa gcttagaaga taattataaa
    44461 tttttttatc ttcattctgt tttgatgtat ctaacaaatg ctccattata ttagtgtatt
    44521 ttttgttttt tgaaggcatg tttaaatcca gataattgtt ttcaatagca aagcatacta
    44581 tattgaaata gttatattta actgcaaaaa ataataacat ttcatcaaat actttatcta
    44641 ttattttgtc agaaatactt atccaaaaat ctggtgactc taagtaaact ttcctactaa
    44701 gttcatctaa ctcttcatct atattattta tattattttc attaactttc atatcatcac
    44761 ttccttttaa atatcttgtg tataaactat ttgttactat tttttatata aaataaaatc
    44821 ttatataaaa atatatcata ttataattta caagtatagt ctaaatagag ctgtttttaa
    44881 gcaaagtttt ataaatgact attaaaatat aaagtaacag tataaagttt acttataaat
    44941 taaatgtaaa cctaaatgct tataatagtt gacaaaaaaa cgtctttatt ttatactaaa
    45001 acaaagcttt aatagaagct ttgtatatgt acatataaac taaatgaaat aagacatatg
    45061 aagggaatta taaaaatgaa aaatactaaa gtaaatattc aagatttaac taaaatgtca
    45121 atatgtgtag cattattgtg tatatcatct tacatatata taccattgcc atttactcca
    45181 gcaggagtaa cagcacaaac aataatgata aatttaatag ctttgatttt aacaccaaga
    45241 caagcattta gtacagttgg tgtatatata atgataggtt taattgggat tccagtattt
    45301 ggtggaggta catctggtat tggaaagtta ttaagcccaa caggtggttt ttattttggg
    45361 tttttgtttg cagcactaat tattagttta ttaaaaggac gaaataatga tattaagaga
    45421 tatatactaa taacaatttt tgttggaatg cctattatat attttatggg aacagtattt
    45481 atgtgctatt ttaatcaaat gacagtagaa gcagcacttt ttgcagcagt agtaccattt
    45541 ttaattggag atacaataaa gtcagttatt gcatcttttt taggcactaa actgaacaat
    45601 gtactcagaa gaaatctatg ataaatctat aatataagga agggtgtaat gtatgaagga
    45661 agtttttata ttaggaggat tgagaagtca tataggtcta aaaaatggaa tttttcaatt
    45721 tgttcaacct gaattactag gagcaagtgt tttaaaagat ttaattaaaa agtatgaaat
    45781 tgataaaatt gatgagatta tatgtggaaa tgcagttggg acaggaggga atatagctag
    45841 attaatgact ttaacagcag gagtgtcaaa tgaagtacct gcttttactg tggatatgca
    45901 gtgtgcttct gctatgatga gtatagacat agctttttca aaggttaaat ctggtcagtg
    45961 tgatttaatt attgcaggtg gttttgaaag tagttctcta caaccaatgc gcacatatca
    46021 taagaatgat aagcgatata atacaaataa tccaaattat acagttgctc aattttctcc
    46081 tgatgataat agccaaaata gtatgctcga aggtgcggaa agagtagcag agttatatca
    46141 aattgagaaa gcagacttgg atttttgggt aaaggaaagt cataaaagag caaaagaagc
    46201 aagagaggaa aaaatacttg aagatattat atctcctata aataatagta cctatgatga
    46261 aggtatacgt gataagatga gtcaaaggtt attagatagg atgccatcaa ttttaggaaa
    46321 agaaactatt acaaatgcag caaattcatg tcttattaat gatggagctt catttattat
    46381 aatatgttct aagaagtatt tagaacatgt aaaaaagaag cctaaagcaa aaattattaa
    46441 tacatgcaca ataggaacag atgcaacctt aagtccaaca tcagcgatac aagcaatgga
    46501 taaactttta gagatagaaa gtcttaatta tttagatgta tctgctgttg aatttaatga
    46561 agcatttgca gttatagatg tgttgtttca aagaaaatat ccagagttaa tagatagata
    46621 taatatattt ggtggagctt tagcgtatgg acatccatat ggtgcttcag gtgcaattat
    46681 tgcattacat cttttaaaag cattagaaaa gactaagggt agatatggaa tttgctctat
    46741 agctgcagct ggtggtctag gttcagcttt actgcttgaa agggtgtaga ttatgaacta
    46801 ttatgaactt ttaagaataa aaaaagagag gcataaaaat aaaaattttt taattataga
    46861 tggagaaaaa tatacttatg aaaatatatt gagttatagt gaggaactag gaaaacaaat
    46921 ttctttaggt gaaaatatcc caattttaat atattctaaa aatataatgt ttcagttgat
    46981 tagttttttt gctattaatt attcaaaaaa tattcctatt atttgtcatt ataatctttc
    47041 taaaaaagta ttagatgata ttttattaaa aaataatatt tcaatcatta tatctgatga
    47101 atctatggac gttattgatt tatatgatga taacaataat gaagtagcaa aaaaagttaa
    47161 tttttctatt tttaatccta aatttaatat atatatgtat caatataaga accttataaa
    47221 ttatttagat aaaagtattt gtatgggagc tttatcttca ggttctacaa gtgttccaaa
    47281 ggtattatat agaacatatg agagttgggc tggatttttt cctatacaaa atggtatttt
    47341 taatatctct ggggaatcaa tcttgtttgt taatggaaca cttagtttta cagggaatct
    47401 aaattctata atgtcagtat tatatgaagg aggtagtatt gtaataagtt caggtcttaa
    47461 ttgtaaatca tggataagga taataaaaca atataatgta actaacatat accttatacc
    47521 tacaaaactt caacttttgg ttaaacactt aaaagaacca gtactcaaag ttgtaagtat
    47581 ttttacaggg tctcaattat tatttgaaga tactgcaaga aatctaaaaa aatatatgcc
    47641 aaagtctgaa attattttat actatggggc aagtgaatta aattatatta catatttatg
    47701 ctatgatgaa ctaattgaaa aacctctaag tgtaggcaga ccatttccag gaatagatat
    47761 atatattaga gaaggaaaaa tttttgtgaa tacagaatat gcagtatata atgcaaccaa
    47821 accttattct gtcaatgata ttggatatta tgataatgat ggatacttaa tatttgaagg
    47881 aagaagtgat gacattttaa atattggagg atttaaagtt agttctacta agattgaaaa
    47941 tgaagttaaa aaaattcctc aaattgaaga tgcaattgta ttgccgtact cagacactat
    48001 aagaggaaat caaattgtat tatttgtaat aactatagac aaaattacga agaaagattt
    48061 gttgattaaa atgaaagata atctaatgaa aaatgaaatc ccaaagaaaa ttatcttttt
    48121 aaattcattt ccatatacct cttctgaaaa gatagataga ttagcattat taaaaatgtt
    48181 ataagtataa tatttagtaa ttttagtgaa ccaatgtatt gataaaaaca tattgtatat
    48241 agtaaaggag gcttacaata tgtcaaatca atacgatata actgttattg ggggagacct
    48301 gcgacaagtg tacatggtaa aaaaactaat taataaaggg tattctgtag tcacttatgg
    48361 gatatcaaat gaaattataa atggaatcgt tggaaaatca acttcactag ctgaagcact
    48421 atccaaaagt tccattattt tatgtccaat tccatttaca aagaatcaag ttaatattga
    48481 aaatacggat gattgtcttg atgctactat tgataacttg ctaattaatt tatctcctaa
    48541 tcaaatatta tttggaggta acataccaac taaagtttgt aaattttgcg agcaacactc
    48601 tattcagatt tatgatttta tgaaaatgaa tgatgttgct attttaaatg cagttgctac
    48661 agctgaaggg gctatagcag aagctattaa aagaagtata atcaatatac atcaaagtaa
    48721 ctgtttagta ttaggttatg gaagatgtgc acaaatactt gcaaaaaaat tatacgcttt
    48781 ggatgcaaat gtatgtgttg gcgcaagaaa taatgatgca agaagtacgg ctaatgcatt
    48841 tggatattcc agtatttctt taagtcaact tgatgaaaaa cttttacaat ttgattatat
    48901 ttttaacacc ataccagagt gtattttaac gaaagaaagg ttagaaaaag tatcacaaga
    48961 agtaactatt atagatatag cttcttctcc tggaggagta gattattctg ttgttaaaga
    49021 agcagggatt aatgcgactc tttgccttgg gcttcctggt aaatattcac caaagacatc
    49081 tggtgaaatt cttgttaacg ctatagaaaa tattataaat gaaagaaggg attagaaatg
    49141 agacttgaag gacttacaat tggagtagga tttactggtt cgttttgtac ttatgataaa
    49201 atatttatag agttagagaa tttggttaaa gaaggagcta atgtacacac tatattttca
    49261 gatgtatcac aaaatatcga ttgtagattt ggaaattctg aagagtttat gaaaaaagca
    49321 tatgaactta caggaaataa acctatagtt acgattgaag aggcagagcc atttggacct
    49381 aaaggaattg ctgatattat aataatagct ccttgtactg gaaatactgc tgcaaaatta
    49441 gcaaatggta ttacagattc accagtatta atggcagcaa aaggtcatct tcgtaatgat
    49501 aaaccactag taatatcaat atcaacgaat gatgctctaa gctttaattt caaaaatatt
    49561 ggcattctcc ttaattctaa aaatatatat tttgtacctt ttggtcaaga taattgcaaa
    49621 gcaaagccta attctatgat tgcacatact gagctaataa ttccaactat agaacttgct
    49681 ctagagaaca aacagctaca gcctgttata aaaagtccac atcaatagaa ttggttctta
    49741 taatattatg tattagatac aatactagca aagaggctgt ctcaaaatag agagaagcct
    49801 ctttttcttg ttgaaatgat gaaaatatat ttaatttcat acaaaaaaat gcctcatgga
    49861 ctaaaaagtc tattttgagc taatctccgt aatttttgct atattaaaat tatttactta
    49921 atccaatttt taaattagaa tttatatcgt tttgactctt caaatatcaa tctaactgtt
    49981 gttgatacaa aagaagagga cataattaaa acaaatactg atagtcctct aataaacaaa
    50041 ttatctacat aataagttac tattccaata cctccaatcg ttaaaattac acttataatc
    50101 aaagaaaaaa cgctaattgt ttttaatttt tccatgctta tcctccttct tataaacttc
    50161 aaatttatct atataatatt aaatatattt tatttcatat tatataaaaa tcatgaatat
    50221 atattttttt aattatacta tatgttgtaa atgtttgtac atttaactat atttacttta
    50281 acttgttaat taagttttaa ttttcatttt caaggatttt tatttttaaa cttgatagag
    50341 tatcatatta aaaattcttt ttataaagtt ttatttgcaa aaaatacaaa aatataaacg
    50401 ttaaaaaaat aacgttatcc ttaagttttt agatgatttc attagtgatt tgcgtaaaaa
    50461 ataaagtgat atctagttaa attaagtatt tgtaaagcta gatattatta gtagaaatga
    50521 tgttatatta gataaaaaat aaaatgttaa aaaaataact tgaaaatagg aaaagtaaat
    50581 gctataatgt taataaatta acataataaa taaaaaacta tctcaggagg ttgttaaaat
    50641 ggctcataaa gaacaagtaa taatagaaaa taaaaaagaa aatgaagtta tgtcagttgg
    50701 tttagatact gtaaataacg ttgaaagctt agttaaaaaa ctagcaaaaa tcagagaagc
    50761 tcaaaaaata tttgctacat atagtcaaga gcaggtagat aaaatttttt tagcagcatc
    50821 tttggcagct aataagcaaa gagttcctct agctataatg gcacaaaaag aaactggtat
    50881 gggaattgct gaagataagg ttataaagaa tcattatgcc tcagagtata tatataatgc
    50941 atataaagaa actaaaactt gtggtgttat agagaaagac gaagcatttg gtatgactaa
    51001 aattgctgaa ccaataggtg ttattgcagc agttgtacct acaactaatc caacttctac
    51061 tgctatcttt aaagcacttt tagctttaaa aactagaaat ggaataattt tctctccaca
    51121 tccaagagca aaaaattcta ctatagaggc tgcaaaagtt gttcttgagg ctgctgtttt
    51181 agctggagct cctgaaggta taataggatg gatagatgag ccatctttag aattaactac
    51241 aactgttatg aaagaagtgg atttgaccct tgctacagga ggtcctggaa tggttaaatc
    51301 agcatattca tctggaaaac cagctattgg agttggtgca ggtaatactc cagcaataat
    51361 tgatgatagt gcagatataa aaactgctgt aaactcaatt cttgtatcta agacttttga
    51421 caatggtatg atatgtgctt ctgaacaatc ggttatagta ttagaaaata tatacaatga
    51481 agtcaaaaaa gaatttaaag agcgtggtgc ttatttatta gataaagatg aaacagaaaa
    51541 aataagaaat ataattttag taaatggagg attaaattct aaaatagttg ggcaaacagc
    51601 ttgtactata gcaaaacttg caggctttga agtcccagta gatactaagg ttttaatagg
    51661 tgaagtagaa tcagttgaaa tagaagaagc ttttgctcat gagaaacttt caccagtact
    51721 tgcaatgtac aaggcaagtg attttgatga tgcagttaga aaagcagaaa aattagtaga
    51781 agatggtgga tttgggcata catcttcatt atatattgat gatgtaaatc aaagagaaaa
    51841 acttgataag tttacttcag ccatgaaaac ttgtagaatt ttaattaata ctccttcttc
    51901 tcaaggtggt ataggggact tatataattt taaactagct ccttctctta ctttaggatg
    51961 tgggtcttgg ggtggaaatt cagtatctga aaatgtagga gttaagcatt tacttaatat
    52021 taaaacagta gctgagagga gagaaaatat gctttggttt agagcaccag aaaaagttta
    52081 tttcaaaaaa ggttgtttag gagtagcatt aaaagaactt aaagatgtaa tgaataagaa
    52141 gagagctttt attgtaacag atacattcct ttacaataat ggatatacta aagcagttac
    52201 tgatttatta gatgaaatga atattaagta cactacattc tttgaagtag aaccagaccc
    52261 aactttggaa tgtgctaaga taggcgctaa agctatgaga gaatttaatc cagatgtaat
    52321 aatatctata ggtggaggaa gtgcaatgga tgcaggaaag ataatgtgga cattgtatga
    52381 gcatccagat gtagatttcc aagacctagc tatgagattt atggatataa gaaaaagggt
    52441 atatacattc cctaaaatgg gtgaaaaagc taattttgta gctataccaa catcagcagg
    52501 tactggttca gaagtaacac catttgctgt tataactgac caagatacag gagtaaaata
    52561 tccattagca gattatgaac tgatgccaaa tatggctata gttgattctg atatgatgat
    52621 gaatatgcca aaaagcttaa catctgcttc tggtatagat gctttaactc atgcattaga
    52681 agcatatgta tctatgcttg caaccgatta tacaaatgga ttagctttac aagctattaa
    52741 gagtatattt gaatatcttc cacgtgcata tgataatggt gcaaaagacc cagaagctag
    52801 agaaaaaatg gcaaatgctt ctacaatggc aggtatggct tttgcaaatg catttttggg
    52861 tgtttgtcac tctatggctc ataaattagg tgctttccac catgtaccac atggtgttgc
    52921 aaatgctctt ttgataacag aagttatgaa atttaattct tcagatgcac caaagaaaat
    52981 gggagcattc tctcaatata aatatccaga agcattaaag agatatgctg aaatagcttc
    53041 atttttagga ttaaaaggaa attctgatga agagaaattc caaagtttat tggtagctat
    53101 tgaagattta aaacttaaag ttggtattcc taaatctata aaagagtttg gcgttgaaga
    53161 atctaagttt atggattcaa tagatgaaat ggtaattcaa gcatttgatg accaatgtac
    53221 aggagcaaat ccaagatatc cacttatgaa tgaaataaaa gatatgtatt taaattcata
    53281 ttatggaaga taagtttgct ataaaataga taaaataaaa actcttatac ataaaacaaa
    53341 gttttattgt ataagagttt ttttgtttga gaaaatggat agtttttatt ctttaattta
    53401 aaattcaatt aaatagtgtt attataataa taaaagaatg tttttatggt tattatgttg
    53461 attttatgtg taacatttaa gaatagttat atacaatata aatgatgaat tggtataaaa
    53521 aggatatatt atctatatga agtattgact aattagggga ggagtcgaat gacagggaga
    53581 aataaaagtt ttacattaat tacaattctt tttttaacat cattattttt gacaatgggg
    53641 tatatctata tagaagatac aaaacatctt cttatgtcag aggcaaaaat acatataaaa
    53701 gaagttgcag tacaaggctc tcaactagca caaagacaaa ttgaaaaaga tttagatgtt
    53761 ttaaatattt tttctaacta ttatgcttca aatccagata tacctaatga ggaaaaaatg
    53821 aaaaatcttt tagatgaaat ggaaaatcaa aaattttaca ctatggcaat agttgatata
    53881 aatggtaatg ctgaaaatac caaaggtgat aagttttcag tgaaagatag agaatttttc
    53941 aaaaactcaa ttaaaggtaa gaagtatgtt tcaagtccat atgttgatga agtaaataag
    54001 tctataaaga agattgctat ttcagtacca ttattaaata atgataaagt agttggagtt
    54061 ttatattgta cttacaatat caatactctg atgaaactta ttaatatatc tttttatgaa
    54121 aataatagta tatcttatgt tgtaaaaaat gatggtacaa ttatccttca tccacaaggg
    54181 gatagcctat caaaaaatat ttataaattg ttagaacagg ataatgatat tcaagaagta
    54241 aatagattaa aaaaagaatt gcaagaaaat aaaacaggtg caactgtatt aaatatgttg
    54301 gaagaaagaa gatatttagg gtatgccact atggataatg ggaatagtga aaataattat
    54361 ataaaagact ggaatttaat tttttcaatt ccagaaactg ttgtatttag taattcaaaa
    54421 caaataatca acagagctgt atatgcagtg ttatctattg tattaatatt tgttgcaatt
    54481 attttttata taatttatat aaaaaaatct aatgaaaaag aaatattaag tttggcttat
    54541 gaagataaag ttacatatat aggaaatcaa aataaatttt atagagaatg tagtaagtat
    54601 cttttagata aaccatcttt aaattatatt atagtatatt ttgatattaa taactttaag
    54661 atgattaatg atacttttgg atatgaattt ggtgacaatt tattaattac aatagcaaaa
    54721 gctttaaaag aagaattaac agaaggtgag gtatatgcac gactttcaag tgattatttt
    54781 gcaattttct gtgattataa aaatggaaga aataaaatta taagaaaact tgataatata
    54841 cgaagtaata ttgaaagtaa tttgagtata gtctttgaaa tttctctgtg tgtgggtatt
    54901 tattttgttg aagaaggtga agtagatatt caaaaggctg taaataaagc aaatatggct
    54961 aggtctgttg ctaaaggtaa aaatataaat tatgctattt ataatgaaga tgttaggaat
    55021 aagcttagtg aggaatcaat gattttagac gatataaaaa tagcattggt aaaaaatcaa
    55081 tttgaagtgt attatcaacc taagttttca cttgtaactg gagaaatgat agggagtgaa
    55141 gcactaataa gatggaatca tccagaacat ggttttataa gtcccgcagt atttattcca
    55201 atagcagaga aaagtaagtt gattttaaaa attgggagat ttgtatttga aagggtttgc
    55261 aatgatttat atgaatggaa aaagcaaggt aaaaagatag ttccagtttc agtaaattta
    55321 tctagggtcg agttgtatca accagatatt gtaaaattta tcaataaaac aataaaaatg
    55381 tataatttaa gttcagattt cattgaaata gagataacag aaactgtggc tattaatgag
    55441 ttaaatattt taaagaatgt tttaaatgag ttgaggacgt atggattttc tatttctatg
    55501 gatgactttg gtacaggata ttcttctatt agttgtttaa gagatatgcc aattgatata
    55561 ttgaagctag ataagtcttt tcttggtggc attgaacatg atgagagaag tcgcaatata
    55621 gcaaaatcaa tagtgtctct agcaaaatca ctagatttag ttgtaattat agaaggtgtt
    55681 gagagtaaag aacaagctga gttaatgaaa caatttggat gtgatttagt tcaaggattt
    55741 tattttgcac gaccaatgcc agctaagaat tttttagatt tactttaaaa caaaatttga
    55801 tttttatata aagcactatt atgaatgctt gattttaata aatcattgct aaataattag
    55861 tgttttttta ttttatcaat gtattgtcta atataaagca aaaaataatt gtattttata
    55921 aaaattatat taaaaataaa tttattttgc taataaacat gttattaaca aaagaatagt
    55981 actataatgt atagatgata aaaactttaa taaaaattta gtgtatagga gttgtttaag
    56041 tatgatagat ataagtattg atgacaaact taaggaaaga tggcctaatg ctaagctggg
    56101 ttgcattcaa gcaaaagtag cagtaaataa aagctcagaa aaattaatta atgaaataaa
    56161 tgagttttgt gatactataa atcaaagtct aaaagttgaa gacattacga aactagataa
    56221 aattaaagat gcaagaaatg cttataaaga gcttggaaaa aatccaagta gatatagaac
    56281 atcttcagaa gcattggtta gaagaattat tcaagggaag ggtctttata caataaataa
    56341 tatagtagaa ataaacaatt taatatcaat aaaatcatta tatccagtag gtacatataa
    56401 tgtaagcaaa ttacattcac cagtatgttt tactgtagga gatgaaggag aacagtataa
    56461 gggtattggg aaagaattaa taaatataga aaatcttcct atattgtctg attcagtggg
    56521 taaatttggg agtccaacta gtgattctga aagagcaatg ataacagaag atgcaaatga
    56581 aatattaatt tgtatatttt cttttagtca agaatctgat attgaagaat attttggata
    56641 tgctaaggaa ttacttaaag aatatgcaga tggaaaagat atagaaacta aaataattaa
    56701 atagaatcta tataacaacg ttatgttaag ctaaaattca atataaaaat aagttaagat
    56761 aaagaaacta acacaaaatt agtttctttt ttaaaatagt agtttagtgt tacaaaaata
    56821 gatagatagt cccacgatta ttaaaagtat ggtaatatat taatatagca gagattgtgt
    56881 ttaagggatt ttgaaagggg ggatttataa taatcatttt atctaaaaga taaaatggtt
    56941 ttatagaaat aactatatat ttaaaaatat aaataatgat acagtaattt gggtatataa
    57001 aataaggcat acatggtttt gtttaaaaac caataaggta cataggtaat tacaatgtaa
    57061 ggaaggattt aattatgatt gatggaaaag aggtaaaaca agaaagtgga caaaaaaatc
    57121 taaaatttaa gatagctata gtagtaggtg ttttgtttgt agcttatata ggaatggcaa
    57181 tcttttttag aaatcatttt tactttggaa cgactattaa tggcgtaaag gcttcaggaa
    57241 aaacagttga gcaagtaaat gatttgttag catctagtac taattcctat actttaaact
    57301 tagaggaaag aaacaataag aaagaacaaa taaaagcaaa agatattgat ttaaaattta
    57361 caccagatga taaagttcaa aagataaaag atagtcagag cgctattgga tggattggtg
    57421 gaatatttaa atctaaagag tataataata tgataactct ttcatataat aaagatttac
    57481 tagaaaaaag gtttaatgct ttatcttgtt ttgatagtaa aaatgttgta gaacctaaga
    57541 gtgcttatcc taagtatgat agcactaaaa acgcttatgt tatagtacca gaggtacttg
    57601 gaaacaaagt gaaaaaagat gatttttata agttagtaca aaactcaatt aattctggga
    57661 atacttctat ttctctggat gaaagtaaag tttatgaagc accaaacaac acaaaggatt
    57721 cagaaaagtt gaaacaagct attaagacat tagataagta tgttggtgta gtcgttacat
    57781 atgattttag tgaccgtaag gaaactttag atggttctga aattaataaa tggttaaaag
    57841 tagactcaga aaagaattat gacataactt ttgataatga tgctatggta gaatatacaa
    57901 gaggtttaag taaaaaatac agtacttttg gtgactcaag accatttaaa actgccacag
    57961 gtaaagatat tacaatttca ggtggaattt atggttggtt gatagataaa aagaaagaag
    58021 ctgaagcatt agttgatgtt gtaaaagctg gtaaaaatgt tacaagagaa cctatttaca
    58081 tacaaaaagc tgtatctcgt aataaagatg atattggaaa tacatatgta gagattagtc
    58141 tttctggcca acatatgtgg ttctttaaaa atggagaaat gcttgtatca acagacgttg
    58201 taacaggtga tttagctaga ggatttgcta caccatcagg tatatatcct cttaattata
    58261 aggctagaaa tgtttcatta actggtcaag gttacagttc acctgtaagt tattggcttc
    58321 catttaatgg gaacataggc attcatgatg ctacttggag aaattctttt ggtggaagta
    58381 tatataagtc ttcaggttca catggatgtg taaatacacc tttttctaag gcaaagaaaa
    58441 tatatgaaaa tatagaacca ggtacaccaa ttattttata ctagtaaatg tgttgctaac
    58501 agttaagcga agtcatccta gtttatttag gatggcttcc tttttatata aagcttattg
    58561 aagtttacag aaagactact taaaaggaga aatacataat gaatgaaaaa ttaactaaaa
    58621 ttttagaaga agctaagaat gatgatgaat ttagaaagga acttataaaa acaagagaac
    58681 aacgtgatgt tatggattca ttttgtaagg ttgttacatc tagaggttat gatatgacag
    58741 taggggaact tttttctatg ggagaagaat atacatccaa tctactgaaa agtgtaaatg
    58801 gaggagcagc atatcctatt gaagattgga gtgattggta cgaggatttt tttataatgt
    58861 tgtcgaatat atagagtaac taatgatatt atctacttaa gattaataca ttgccttaaa
    58921 ataaaactat tagaactagg tgtaagtttt atcaatcttt aaaaatattg atttatttct
    58981 aactagatat aaaaaaatta gattaaaaat aaggtgacaa aaatcgatat agcttgatga
    59041 tttataaaag ttagataaaa tagtgcgaga tatttctaaa aattcaaaaa aaacaataaa
    59101 cttcaatggt tagaggttga aaaagaatat aaaaaacttt tatatgtaat aaattaaatt
    59161 aaaattataa aaaaaataaa ataaaaattt aaaatgtcga cataacgtta attttaaaac
    59221 aaaataacgc tattgacaaa ctatatcgct ttaaattata attattgaaa ataatggtat
    59281 ttaaaaacca tactcatgtc atagtctaaa agtaagtcaa aaaaggatgt aaatctaggc
    59341 aagtactatt ctaaaatatg ttttattaag aaataaacgt tgaaaaatat taattattat
    59401 ataaaaatgc tgtttatact ttaacaatga ggtagtaatc taattagaat aaaaaaatct
    59461 aattggttat atacatagta aaaaggtaag agaggtgatt tggatggctt ttgaaataat
    59521 aaaaagcatt gttgaggcag agcagacagc agacagtatc aaagtaaaag ctgttactga
    59581 tgcagagtct atcagagctg atgctgtaaa caaatgtgaa agcatatttg ctgatgtaaa
    59641 aaaacaagca aagcttatgg aagaaactct tattgagaag gcagtcaccg acagtagagc
    59701 agaggttgat aaaatcttag ctaatgctaa aagtgaatgt ctgaaaattg aaaaaactgc
    59761 tgaagaaaga aaaagtaagg ctattgaagc ggttattgga aaggtagtga gataatatgg
    59821 caatagttaa tatgaacaaa atatctattg ttggcttaga agctcaaaaa agccagattt
    59881 taaagctact tatgaaccgt gggtttgttc agattgatga tagtgccttt cttactgagg
    59941 aggaggaatt aaaaaattat cttgtaaaag atagtgaaga atctgaagtt ataatgctag
    60001 agcagaaaat gtctcatgta aaccaagcaa ttaatattgt ttcttcacta gtgaaacaaa
    60061 aaaaatccct acttgcacca aaaagagagt ttaacaaaat tgatagtgaa aaagaagggc
    60121 aagtttatgc tgaagctctg gagttaaaca atttgcacaa ggagataggt acaataaggt
    60181 ctaacataaa cttacttaac aacaataaaa gtatgttaga accatgggtg aactttgata
    60241 ttcctattga aaatatggaa actaagtata caaaaactat acttggaact gtgcccataa
    60301 gcgcaaacat tgatgcttta agagtaaaat tagaagatga aataccagaa agtgttattg
    60361 ggattgtcaa ttctgacaag ttaatgaact atgtttattt aattacttta aaagaaactt
    60421 ttgatgatgt tttagaagtt ttaaaagaat ttaatttttc agtggtttca ttaccaaatg
    60481 aagaaggtac accgttacaa gctatcaaga aatacaatga aatgataatt agacaggaaa
    60541 acgtttctaa ggacaaaaca gaggaaatta aaagacatgc agatagtata cacatgcttg
    60601 aaaacctata tgatttttac actatagaaa gagaccaaaa aaagattata gagagacttg
    60661 tcaaaacaaa aacaacattc tgtcttaatg gttggttacc aagcaaaagg gcagatgaat
    60721 taattaaaga aattacagaa aagtttgatt gttgtgttca gactgaagaa ggaagtaaag
    60781 aagaaggctt cccaattctg cttgaaaaca ataaattagt aacacctttt gaaagtgtaa
    60841 ctaacatgta cagctatcca agtacaaaag atattgaccc aaatacaatt ttaactttct
    60901 tctttgttat attctttgga atgatgctaa gtgacgccgc atatgggtta ataattgcag
    60961 tagtttgtgg atttgttgta tataaattaa agatacaaaa aggtgaagga aatttaatta
    61021 aacttattgg tatctgtgga gtttcaacta ctgtatgggg tcttatattc gggggtattt
    61081 tgggggattt aattcctata aaagcattaa ttaatccttt agaggatgtt atgacactta
    61141 tgggaatgtc gttactgttc ggtattatcc atatatatgt aggtttggga ataaaagcat
    61201 atacattaat aagaagtgga aaggtaatag atgcaatctt tgacataggt ttttggtatt
    61261 tgtgtataac aggtgtatgt ctacttataa taccttttgt tgcaggggac ataggtgtat
    61321 tttcagaagt aggaaaatat ttagcaatta taggtgctat aggtcttgta cttactcaag
    61381 gaagaagctt taaaggtatt cctgttaagc tttttaaagg attttcaagt ctctatggta
    61441 taacaagtta ttttgctgat atattatctt atacaagaat tatggcgctt tgtttaacaa
    61501 caggagttat agcacaagtt atcaaccttt taggggctat agctggtccg atacttgcag
    61561 ttgtaattgg tgttgtagga cacacaataa accttcttat aaatgcacta ggtgcatatg
    61621 ttcatacaag taggttacag tatgtagaat ttttcaataa gttttatgaa ggcggaggag
    61681 ttccttttgt tccgtttaag tataaaacaa aatatacaag cattaataaa aaggagatgt
    61741 aaatatggaa tttttaaacg cgttaggaaa tggtcaattt ttggcaatat caggagctgc
    61801 tatagcagca ttatttgcag gtatgggttc tgctaaaggt acttcaatag caggtcaagc
    61861 cgcagcaggt gttgtaacag aagaccctag taaattcggt caacttctag ttttacaact
    61921 tcttcctggt acacaaggtc tgtatggatt tatcatagct ttccttattt tatcaaaagc
    61981 agggattatt ggtggagatg caataccaac atcagctcaa ggtttacaac ttcttatggc
    62041 aggacttcca attggtttag ttggtttagt atcaggtata gcacaaggta aagcagcagc
    62101 agcaggagtt ggaatagttg caaaaagacc tgaagaactt ggtaaagcta taacatttgc
    62161 agcaatagtt gaaacatatg ctcttttagg actacttgtt tctttcttag cttacaatgg
    62221 aattgcaatt ggttaattat aaattataat tcataagtaa aaaaaatgaa aaggaggaag
    62281 cactcctatg ggtaatgaac aaaaaatgat tgacagaatt atagctgatg ccaaacaaga
    62341 agcacaagaa atattggata aagctaaaag tgaagcagat ttaaaagtaa attcagctaa
    62401 tgaaaaagct gaaaaggaaa tggcttccta tacaaagctt gcagaggcag aagcagaaaa
    62461 agcggcttct aaggaaatat caggagctta tatggaggct aaaaagcaaa tattatcaaa
    62521 aaaacaagag attttagaag aggtaatttt agaagctaaa aacaaacttt taaatcttaa
    62581 agataatgaa tatgaagaga ttatactaaa catgattgaa aaatcaaatt gtacagatga
    62641 ttcagagata gtattatcta aaaaagataa aaaaacatta aaagatgtgc tatcaaaaaa
    62701 aggcattaaa gtgtctgatg aaactagaga tattactgga ggtttcatag ttaaaaaagg
    62761 tgacattgag tacaattatt catttgaagc cataattgca gtagagcatg aatatattga
    62821 acaaattgca gcagaaattt tgtttaatta aggaggggtt aaaaaatggc agaagaaaaa
    62881 acttatccct atgccgttgc acgaataaga gtgcttgaaa aacagcttct taacagacaa
    62941 atgtttattc aaatggcaga agccaaaagc cctgaagact ctttaagaat tatagctgag
    63001 gcaggttatg ctgatacatc aaataataat atacataatt ttgagaatat tttgactcaa
    63061 gaattatcaa agacatatga aattcttaaa agtctagctc ctgaagaaaa atttgttgat
    63121 gtatttcttt ataaaaatga ctatcacaat ttaaaagtgc tgattaaaga agaaatttct
    63181 ggtgtagatg gtgagaaata tttaattgat ggaggtacaa ttcctcttat taagcttaga
    63241 gaatctttag caaatcgtag ttttagtgat ttacctaaga taatgtcatc agctatatta
    63301 gaggcatttg atgtttataa taagacacaa aatggccaga tggttgatat tgttctagat
    63361 aaagcaacct ttaccagtat gaaagaaact gctaaagaaa gccaaaacaa atttgtaata
    63421 gattatgtta tgaaagtttg tgatttaaca aatctaaaga gttttataag agtcaaaaat
    63481 atgaagaaag attttgatat gtttatgaat gtgtttgttt caggtggctc acttgataag
    63541 gaaaaattct tagaagcttt ttcctcagat actccagcat catgttttaa atctacttct
    63601 tactctgatg tctgtaaaaa tggcatggat agtggtttta ctgtctttga aaaactttgt
    63661 gatgactatc ttatggaata tgttagagga gcaaaattta agcctcttac attagaacca
    63721 ctaattgcat atttatatgc taaagaatca gagataaaaa caattagaat tattttgact
    63781 agtaagctta ataatataga cgcagatact ataaaagaaa ggctaaggga tgcttatgta
    63841 taaggtagga gtagttggag ataaagatag catcatggga tttttagctc ttggaataga
    63901 tatcttccca gcctatgata gtgacgaaat taaaaaaagt atacataagt tggttgaaga
    63961 tgaatatgcg ataatttata ttacagaaca ggcatcattg cttgcaaaag aatcaatagc
    64021 aaaatacaaa gattaccaac ttcctgccat tatagttatt ccaggtattg gaggaagtat
    64081 gggattaggt atgaatgaag taagagaatc tagtaaacgt gctataggtg cagatatatt
    64141 atttaaagaa tagatgaaag ttgaataaaa gacaatctca aattttattg gtgctaaata
    64201 tatgtgctaa taaaatatag gagggaatat ttaaattttg ataaagtata tgattgcagg
    64261 tgagagaata tgaaaacagg cacaattgta aaggtgtcag gaccgcttgt tgtagcggag
    64321 ggcatgagag atgcgaatat gtttgacgtc gtaagagttt cagataaaca tcttataggc
    64381 gagattatag aaatgcatgg agataaagct tctatacagg tttatgagga aacttccggt
    64441 ctaggaccag gggaagaagt agtttctctt ggaatgccta tgagtgttga acttggacca
    64501 ggtcttattt caactattta tgatggtatt caacgtccac ttgaaaaaat gtatgatatt
    64561 tcaggtacta atataactaa aggtgttgaa gtatcttcac ttgatagaac aactaagtgg
    64621 gaatttaaac ctaaaaaatc tgttggtgat aaggttgtag caggagatat aattggtggc
    64681 gttcaagaaa cagcagtagt agaatgtaaa attatggttc cttatggagt taagggtact
    64741 ataaaagaaa tatattctgg tgaatttact gttgaagata cagtttgtgt tattacagat
    64801 gaaaaaggta atgatatacc tgtaactatg atgcaaaaat ggccagttag aaaagaaaga
    64861 ccatatagag aaaagaaaac accagattca cttcttgtta caggtcaaag agttatagat
    64921 acatttttcc caatcacaaa aggtggggta gcagctatac cgggaccttt tggaagtgga
    64981 aaaactgtta cacagcatca gcttgctaaa tgggcagatg cagatatagt tgtatatata
    65041 ggctgtggcg agcgtggaaa tgaaatgaca gatgttcttc ttgaatttcc tgaattaaaa
    65101 gacccaagaa caggcgagtc acttatgcaa agaactgtgc ttatagcaaa cacatcagat
    65161 atgccggttg ctgcacgtga agcttctata tacactggta ttacaatagc tgaatatttt
    65221 agagatatgg gatatagtgt tgcacttatg gcagactcta catcaagatg ggctgaggct
    65281 cttagagaga tgagtggtcg tttagaggag atgcctggtg aagaaggtta tcctgcatac
    65341 ttaggttcac gtcttgctca attctatgag agagcaggaa aggtaaattg tctaggtatg
    65401 gatgaaagag aaggaacact tacagcaatt ggtgcagttt ctcctcctgg tggtgataca
    65461 tcagaacctg tcagccaagc aacgcttcgt atagtaaaag tattctgggg tcttgatgca
    65521 tctttagcat ataaacgtca tttcccagca attaactggt tgactagtta ttcactctat
    65581 caggaaaaag ttgatgtatg ggcagataaa aatgtaaatg ataatttctc agctcaaaga
    65641 gctcaagcta tgaaactttt acaagttgaa tctgaacttc aagaaatagt tcaattggtt
    65701 ggggttgatt cactttcaga tggagataga ttgatactag aggttactcg ttctataaga
    65761 gaagacttcc ttcatcaaaa ctctttccat gaagttgata cttatacatc acttcataaa
    65821 caatatagaa tgatgggact tatattaaaa ttctataagt cagcacaaga tgctatcaag
    65881 caaaatgtta ctataaacga tattatcaag ctttcagttc ttgaaaagat tggtcgtgct
    65941 aagtatgtag aagaaggcga tatagatgca gcttacgatt caattgaaga tgaaatagac
    66001 aacgagcttg cagaccttat taaaaagagg ggggctgaag aactatgata aaggaatata
    66061 agtcaataca agaggtagcc ggtcctctta tgttaataaa cggcgtagaa ggtgtaaaat
    66121 ttgatgaact tggagaaatt gaactttcaa atggagaaat tcgtcgttgc aaagttctag
    66181 aggtaaatgg agatactgca cttgttcagc ttttcgaaag ctcaacaggt ataaaccttg
    66241 ctgaaagtaa agttcgtttt ttaggaaaga gtcttgactt tggagtttca ccagatatat
    66301 taggtcgtgt ttttagtgga atgggtagac ctattgatgg aggtccagaa attataccag
    66361 ataaaagact tgatataaat ggagctccaa taaatccagc agcacgtgat tatccggccg
    66421 agtttataca aactggagtc tctgctatag atggacttaa tacattggtt agagggcaaa
    66481 aacttcctat attctcaggg tctggtttgc cacatgccaa tcttgcagtt cagattgcaa
    66541 gacaggcaaa agtacgtgga actgattcta agttcgccgt tgtatttgct gcagttggta
    66601 taacttttga agatgctgaa ttttttataa gcgattttaa agcaactggt gcaatcgacc
    66661 gttctgtagt gtttgtaaac cttgcaaatg acccagctgt agagcgtgta tctacaccta
    66721 gaatggcact tacagcagca gaataccttg ctttcgagca agatatgcat gtacttgtta
    66781 taataacgga tattacaaac tatgcagagg cattgcgtga agtttcagca gctaaaaaag
    66841 aagttccagg acgtcgtgga tatccaggat atctttatac tgaccttgct actatgtatg
    66901 aaagagcagg aaaaatgaaa gggcatgaag ggtctataac tatgattcca atacttacaa
    66961 tgcctgagga tgataagaca catccaattc ctgaccttac aggatacatt acagaagggc
    67021 aaattatact tagccgtgaa ctttataaaa aagatataca gcctcctata gatgttttac
    67081 catcactttc tcgtctaaaa gataaaggta ttggtaaagg taagactcgt gaagaccatg
    67141 cagatacaat gaaccagctt tttgcagctt attcaagagg taaggatgca aaagaactta
    67201 tggctatact tggtgaatct gcactttctg agattgattt aatgtatgct aagtttgcag
    67261 atgaatttga gaaagaatat gtttcacaag gatttagaac tgatagaact attgagcaga
    67321 cattagaaat cggatggaag cttcttagta tgttaccaaa aagtgaactt aaacgtatcc
    67381 gtgatgaata ttttgaaaaa tatatgccta aggagtgata tctgtggcta gattaaatat
    67441 aaatccaaca cgtatggaaa tgactagact taagaaactc cttaaaactg ctacaagagg
    67501 tcataagctt cttaaagata aacttgatga acttatgaaa caatttcttg agattgtaag
    67561 agaaaataag cgattgcgtg aagaagcaga aaatgctctt gatactgctt ataaaaactt
    67621 tataatcgca agagcagtta tgagtcagga gtatctgggt tcagcactta tgatgccaaa
    67681 gcaatcagtt tcagtagatg tttctacgag aaatataatg agtgttgatg ttcctgtgtt
    67741 tgactttaaa actgaaaaca atcaaagtga tatttatcct tatggtcttg cttttacatc
    67801 aggtgaatta gattctgcta tggaagcttt ctcagatgct atgcaaccac ttttaagact
    67861 tgcagaaagt gaaaaaagtg cacagcttct tgcacaagag atagaaaaga cacgtagacg
    67921 tgttaatgct cttgaaaatg taatgatacc aaattacatt gagactatta agtatatagc
    67981 tatgaagctt gaagaaaatg agagagcaag tactactaga cttatgaagg ttaaggatat
    68041 ggtgcttaaa aaagcacttg aagaaaagaa aaaaaatgat ttggttgtat aattaaatac
    68101 ttataaaaat aagtctaaca ttctacttta tagaggttag acttattttt tatgtttaaa
    68161 ttaaaatgta gatactataa aaattaatat attgtttaaa aacagactgt atttaactaa
    68221 aaataatata tttaacaaaa atttctaaaa attaacgaac tgttaattta cacttaactt
    68281 tatattaaca aattaggtgt attatataaa cataagcaaa aacaataaaa aacaaataaa
    68341 aaaaggaaaa aacacaaagg gggaagatat gaaagaattt atatttactt gtctgtgcat
    68401 tgtagtgatt acatttatga caagttgcat atcaacaaca ccacaaacta ctcaaggaaa
    68461 aggaaataat aaacttacaa tatatgcagc actagaggaa gagcagatag cttattatat
    68521 ggaaggtttt agagaagagt atcccaatat agatgtagag atagttatgg attctcatgg
    68581 tgtaatagct tcaaaactat tagcagaaaa agataatcca caagcagata ttatatgggg
    68641 attatcagca atgaacatga tagagctaaa aaaagatggt gcacttgaag catataagcc
    68701 taaaaattat gataaagttg ataaaaaatt taaagatact gaagatgatg tatcttgggt
    68761 aggaatgagt gtcccagaga cagcaatagt agttaattat aaggaactta agaaactagg
    68821 tatagatatt ccaaaaagtt atgaagattt aataaaacca gaatataaag gacttataac
    68881 aatgccaaat ccagcatcat ctggaacggg gtatcttaca gtatcagggc ttattcaaat
    68941 gatgggtgaa aaagatgctt acaattacat ggataaatta cataaaaata tagccacata
    69001 tacacactcg ggttcaaaac ctgcgaagtt agctggctct ggagagtatc caataggtat
    69061 tacacttgga tatagagcta cacaacaatt atctagtgga gaacctatag aggttgtatt
    69121 tccaaaggaa ggttcaggtt gggacttaga agcaaatgcc ttagttaaaa aagataatat
    69181 aaaaaaagaa gcaaagctat ttttagattg ggcaataagt gattcagcta tggatagata
    69241 tgcacatgaa gtagcagtta cttccataaa gacagataat ccagttccaa aaggttattc
    69301 taaacaacca tttgaacagc ttataaaaga agttgattta aactctgctg ccaaaaacag
    69361 aagtacagta cttaaaactt gggaatcaaa atacggttca aaatctgaaa gtaagtaaag
    69421 tttaaattaa taatttagat gaaagaattt tagaagaaaa agtaggatat aaaatttaaa
    69481 ataaaaaaat aactatataa ggagagataa gatatgagtt acttaaaaat aaataatgtg
    69541 tttaaaagtt atgaccaaaa aaaggtttta aataatatta gtttggatat tgaagaaggg
    69601 gaatttctat gtttactcgg acctagtgga tgtggtaaaa ctacattact tagaataata
    69661 gcaggtcttg aagatgtaaa tagtggaact ataattctcc aggataaaga tattacaaat
    69721 ttagagccat caaagagagg ttttggtata gtatttcaat catatgcatt atttccgaat
    69781 atgactgctt ataacaatat agcttttcca ctaaaagaga gaaaagtttc aaaagaaaaa
    69841 atagataata aagttaagga agtacttgaa acagttggat tgactaatga agctcataaa
    69901 taccctaaag cattatcagg tggacaacaa cagaggattg caatagcaag agcattagct
    69961 cttgaaccaa agtttttact tttagatgaa ccaatgtctg cattagatgc aaaggttaga
    70021 cacaagctta gaatggacat taaaagatta caaaaagaac ttaatataac gacaataatg
    70081 gtaacacatg accaagaaga agctataact atggcagata aaatagctat tctaaatgga
    70141 ggagatataa tgcaaatagg gactccagaa gaaatatacc aaaatcctca aaacctattt
    70201 acagctcaat ttataggaga tacaaactgt tttgataatg gagattctat attaactgta
    70261 agaccagaat atgttcaaat agagaaatcg acaaaagaaa actaccaagg gataatatca
    70321 aatatagagt ttagagggaa tttattgaga gttgaaataa aagataaatt aaatgaaaat
    70381 ttcataataa gtgatgtttc tataaaagag tgggttaatt taaatttagt agaaggtgac
    70441 cttgtaaaaa taagtataga cgaaaaatac tatttaaagt atccaaaagt taaaggagca
    70501 taagtgtatg aataataatg ctataagttc taaaagtata agtcttaaat caaaaatgaa
    70561 tttagatata aaaaaatgta tgcttacagt attagttatg tctttagtaa tatttttatt
    70621 agcacctata atatatctat ttgtacaggc ttttttagat aaagatggag tctttgtagg
    70681 attacaaaac tttcaagaat acttggcttc tgaatctcta ttaaattctc taaaaaatac
    70741 agtatttgta tcagttatat caactgtaat atcactaaca cttgcattag tatatgcata
    70801 tgcattaact agaacaaaga ttaaatttaa aggttttttt aaatcattag ctatgtttcc
    70861 tatgtttgct ccaacaatga tgcatgcaat aggtctagta tatttatttg gaaatcaagg
    70921 tttggtaact aatttacttg gagtgcatgt tccaatatat ggagcagtgg gaataatatt
    70981 ggcacaaatt atatatgtat ttcctcaatc atttcaaata ctttatatat ctctaagttc
    71041 aaccgattat agactatatg aagctagtaa tactatgggg ataagcaaga taagagaatt
    71101 tataagcata acaatcccaa gtataaaata ctctttaata agttctgcat tcgtatcgtt
    71161 tacattatgt tttacagatt ttggagctcc aaaggtgata ggaggagagt ttaatgtatt
    71221 atcaatagat gtatataagc aaattatagg gcaacagaat atgccaatgg gagcaactgt
    71281 atcaatatta cttttaatac ctgcaataat atcgtttata gtaactaaaa aaattgaaaa
    71341 aaatcaaaat tcatatataa cttcaaagtc aattccatat aaggttaaaa ctaataagtt
    71401 aagagatagt attttaggag gggttgtagg aattattgca tttggtataa tagctgtaat
    71461 gttaactgta atcttatctt cattaatatc agtttggcca tatgatttaa gactgacact
    71521 ggaacatttt tctatgaaaa acagtattga tgggattaaa ccatttataa attcaataaa
    71581 aatgtcacta tttacagcta ttctagggac gagctttgtt ttcttatttg catatttgaa
    71641 tgaaaaaaca gaaaaagctc catatttaaa atcatttggg tattttttaa gtacattgcc
    71701 tatggcattg ccaggattag ttttaggtat agcttatgta ttatttttta ataaattaga
    71761 gtttaatttt atggatacaa tatacattaa gaatttgttt aatggattat atggaacatt
    71821 acttataatg gttatatgta atatagttca tttcttttca gtagcattta taacagctac
    71881 tactgctatt aagaaagtag ataaagaatt agatatggta gctaaatcaa tgggaataaa
    71941 cagcgcatca attcttaaga acgtaactat accactttgt ttgccagcag ttttagagaa
    72001 ctttatgtac tttttcctaa actccatggt gacaatatca gcattagttt ttatatatac
    72061 tgcaagttca aaagtagctg caatatcaat tgtacaacta gatgataagg ggagtactgc
    72121 acaggcagca gcactagctg tgttaatatt agtcacaaat atattagtga aagtaatata
    72181 tgaatttata aagaatcgtt tagaaaaaag aacaaacttt aattaaataa tttatcttat
    72241 aaaaacacaa atttaaaaaa gtctattctt gtgatttatt atttaaagaa tagacttttt
    72301 aaatttatta ttgatattgt ttattaattt tccttttgtt atcatacatt tgagaatctg
    72361 cacgacgata tgtatcaaat aaagaaacat ctactttttc atcaaaaata gcatatccaa
    72421 tagacattga taattttaat tcattagttg agttttcttc aactattttt aaattaatat
    72481 tatttataat attatttatt tctttgtgag ataaattttt tagaataatt atgtattcat
    72541 cacctccaat acgataaact atatattttt tatttattac atattgtata attttacttg
    72601 cttgcttaat atataagtct cctgtctgat ggccaaattt atcattaatc ttttttaaac
    72661 cgtctaaatc tattgatatg atagatatta gattctttag atttgaattg tttaagttat
    72721 tcatatctat ttcaaaagca tttctatttt ttaggcctgt taaataatct gtattagcaa
    72781 aatcagtata tgtaggatta gatattcttt taaatagaat cactgcaatc aatgctgata
    72841 ttagacaagt taataatgct attattggtg taactatact tataaattta aatgtattgt
    72901 attgatgttt tgcttcaaat tcaattccta ttactccaac aaccttacca acatcattgt
    72961 gaataggaaa atacgatata aatatgtatc cccaagatgt tgatttaatc tttttaggta
    73021 acaatatatg attatctaat gctttgttaa ggtctcccca aatctctttt tctatcaaat
    73081 caccagcatt tctaaaatca ctcctagatg aatttaaacc atcaacaaca tatactagtg
    73141 atccatcttt tgttttttta gcagtatata gatacatgac tccagtagta ttttttacag
    73201 attctaaaag gattttagaa cttttataag acttttttga ttggtcttct ttagaattaa
    73261 tttcagtaaa ggtattttta tcaatatttc tttctacata ttcatagata tgttttgacc
    73321 tctcacttaa agagtatatc atgctcttat aagttagtgt ataattaaaa aaatatacac
    73381 atgaggaaga tattattaca ataattgcag taagtattga aacctgcata tcaactctat
    73441 tgaatttttt atttattttc atagcttaaa tgtcttgtga aaagaacctc ccacaatctc
    73501 cccataatta atatatatct ataaatttca ccataatata ttcatatatt atcaaatttg
    73561 tgtggaaatt acaattagta aacatgctta aagcattcaa atatagtttt tttattatta
    73621 tagtatacta ttttatgtat aaataaaatt atatataaat cattgatatt ggttgtttat
    73681 taatggaaga taccgtataa aatattctaa ttggttacga tatgtatact taattgaagt
    73741 taagttaagg gttagaatat gtattgatga aatggctaga tttatattca taagtattaa
    73801 ttttatcata gataggtaaa atactgtatc ttaaacataa gtatgattga gatgcaatcc
    73861 tgagatatga taatataaat tgaaggatag tgaactcaat caaataaaat gattaatcta
    73921 gctatataag caaataaatt taatataaaa gaggtggaaa atgaaagaca gacttaattt
    73981 aacagaagga agaattacag aaaaattact taagctatca cttccaataa tggggacttc
    74041 atttatacag atggggtata acatgataga tatgatatgg gttggtaaag ccggaagtaa
    74101 agcagtagct gcagttggaa cagctggttt ttttccatgg cttgcaatgg catttattat
    74161 gatttcaaaa gtaggtggag agataaaagt agcacaaagt attggtgcaa ataatataag
    74221 cactactaaa tcatatatga agtcagcaat agaaataaat attatactag caataatata
    74281 tacaatttct ttaatagtac ttaataaaca attgataggt ttttttagat taggagactt
    74341 agaagttata accatgtcta gacagtattt gataatagta gctctaggta tggtatttta
    74401 ttttattaat ccagtattta ctgcaatttt caatggtctt ggaaatagta aaactccttt
    74461 tcgtattaat acagtaggat taataactaa tattatactt gaccctatat taatttttgg
    74521 atggggattt gctccaaaac ttggtgttgc aggagctgct attgcaactg tatttgctca
    74581 gatagtagtt actacatgtt ttatatatat aatagttaaa agtaaagaag aatattttaa
    74641 attaagacta tttaaagaaa tagagactaa atattataaa gttctgtata aattgggatt
    74701 acctatagca atacaaagtg gtatgtttac agtattctca atgctattag gagtaatagt
    74761 agcatcatgg gggcctgtgg ctgttgcagt acaaaaagta ggctcacaaa tagaagcaat
    74821 ttcctggatg acggctgaag ggctatcagt tgcattaggc agttttgttg gacaaaatta
    74881 tggtgccaag aagtattcta gaataaataa aggttgcaaa atagctataa ctgtatcttc
    74941 tgtacttggt ataataacta ctttagtatt agtgtttgca ggagagagta tattttctat
    75001 ttttatagat gaaagtgagg caatagaaaa aggagcagtt tatcttaaaa tattaggcta
    75061 ttctcaattt tttatgtgtt tagaaataat aacaacagga gctttcaaag gattagggag
    75121 aacctatata ccttctatta taattactat actgactggt gcaagagtac cattggctta
    75181 ctttatatct agaccagaaa tgttgggtct aaatggtgta tggtggagta taaccttatc
    75241 aagtatatta aaaggtatat taatgataag tctatttata tctttattaa aactcggaaa
    75301 gttgtacaag atttctgaat aaaatttaaa ttatcttaaa tgtatagaca ttttctttgt
    75361 ttgtatataa aattatctta ttatataaaa gatttaataa ggagtgaatt aaatgaaaga
    75421 gccaatttat aaagttatag aaaatcatgt tagagagtta ataaattcag acagtttgaa
    75481 agaaggggat ttaatccctt ctgaaaaaca gctgagtgaa gaatttaatg taactagaat
    75541 gactgtaagg tctgcactta ataatctagt taaagaaggc tatataacta gacaaagagg
    75601 tgtaggtagt atagttattg caaataatat atatgataat atatcgtctg taagtggatt
    75661 tactaaagag atggaaagta aaggctataa agtatctaat atattggtat ctcttgaaat
    75721 agttcaggca gatgaaaatt tatcaagtaa gttgaatata agtttagaag aaaatgtttg
    75781 ggaaataaaa agagtaagat tagccaatga tgcaagagtt tcatatatga ttacttatat
    75841 gccagttaag ttatttccaa atttaaataa gactcattgt gaaaattctc tttataattt
    75901 tgttgaagaa gtgtgtgaat acaagatagc catgtcagag agagaagtac aagctgtaat
    75961 atcgaatgaa gagtgtatgg ataatctaaa attagaagaa ccagagccac ttttatatat
    76021 aagccaaatt tgtaagttac aaaatagtga gatttttgag tactcacaca catatcacta
    76081 tggatataca ttaactttga atgctgttgt cgaataaaat ttaaataaaa tgaaaaagtt
    76141 ttcttgaaat gtatatacat ttggtatata ataatatcaa gaattaaatg tatatacatt
    76201 taaaaataag atatatacat taagaaaaaa ttaatagaaa aacatatttt aaatgtatag
    76261 tttaaaaaac ttatgactta aaaaatattt aatgaatcta atttggaggg gaaaaaatga
    76321 taaaaataat gttagcttgt tcagcaggaa tgtcaactag tttattagtt acaaaaatgg
    76381 aggatgcagc aaaggaaaac ggtatagaag caaaaatatg ggctatatca gaagtgaact
    76441 taaaaaatga aatagaaaat tgtgatgtct tacttttagg gcctcaagtt agatatgtat
    76501 taggaaaagc aactgaaatg gcaaaaccat ataatattcc agtagaagtt ataaatatga
    76561 tggattatgg aagatgtaat ggaaaagctg tgttagatag agctgtagag ttaaatagtt
    76621 caaaataaag ttgtactgga ggatgtaaaa tggaaaaatt catgtcattt atggataaat
    76681 atatagtgcc tgtagctgct aaaataggag ctcaaaggca cttagtagca gtaagagatg
    76741 catttatagt aatgatacct ataactatgg taggtgcatt gggaacattg ataaataact
    76801 taccattgga agcatacaaa aatttgatgg caagtatatt tggagaaaac tggaccacat
    76861 ttggaggcga cctttggtgg ggagcaatag gtacaatggc agtattctta gtaataggtg
    76921 ttgcttattt tctagcaaaa tcttatgaga gtgatgggtt acaatctggg cttatagctt
    76981 tatctatctt ttttataatg gctccacaaa ttggtaaaat agttcctgag ggaggaacta
    77041 cagtagttga aggatgggga atgatacaac aaacctactt aggtacagca gctttattct
    77101 cttctatatt aataggatta ttatcaactg agatatttgt aagattatct aaggtaaaga
    77161 aattaactat aaaaatgcca gatggagttc caccagcagt ttcaagatca tttgcaaagt
    77221 taataccagg aatgttaact ataatgatat ttactgtaat aggaatattt ataaaaatgc
    77281 tttctaatgg aagtttctta acagatattt taaatactta tttaggagca ccactatcaa
    77341 atgtagctga tagtctaggt tctacaatgc ttattgcatt tataattcat atactttgga
    77401 ctgttggact tcatggtgca aatatagcac ttccttttac agaaactata cttatgaaat
    77461 taggtggaga aaatgctgcc ttggctcaag caggagcaac ggaaggatac catgtacttg
    77521 caggtgcatt ctttgatgca tttgtatatt taggtggttc tggaatggta ttgggattaa
    77581 tagtagcttt attaatagct ggaagaagac gtaaagagat gatagtatta ggtggtccac
    77641 ctgcaatatt taacatagga gaaccattga tatttggact tccaatagtt ttaaatccta
    77701 tatttatgat tccatttgta cttgcaccag taatatgtag tgctgtatca tacttagcaa
    77761 tagattttgg attagtagca ccagtaattt tacctaaaat accttgggta acacctccta
    77821 tattaggtgg agcaatggct acaggtgact ggactggcgg agctttagca ctatttaatt
    77881 taatattatc aatattaata tatattccat ttgtcatagc gtctgaaaaa atggaagcta
    77941 ataaattgaa aattaacaac taggtagttt tatttcggta aataaatgta tagagtttta
    78001 aataaatgta tggggttttg tagagtatat taaattatag gtaaggatgg tataatgaga
    78061 tgaaaaaggg actaaaaata gttacaattg gaggaggttc aagttatact cctgagcttg
    78121 tagagggttt tataaataga tatgaagagt tacctgtaaa ggaattatgg cttgtagata
    78181 tagaagctgg aaaagaaaaa ttagagatag ttggaaatct agctaagagg atggtaaaaa
    78241 aagctggagt agatatgaaa ataaatctta ctttagatag aagagaagct ctaagagatg
    78301 cagactttgt gacaacacaa cttagagtag gtcttttaga tgcgagaata aaagatgaaa
    78361 ctattccttt atctcatgga gttatggggc aggaaactaa tggtgctgga ggtttattta
    78421 aagcccttcg cacaatacca gttatatttg atataataaa agatgtggaa gaattatgtc
    78481 caaatgcatg gatggtgaat tttacaaatc caacaggaat aataacagag gctgtattca
    78541 aatatactaa ttttagaaga tatataggtc tatgcaacgt gccaattcat ttaaaaaatg
    78601 atgttgccaa actatttaat gtagaaagtg acagaataag tatggatttt gcaggtctta
    78661 atcatatggt ttatggtcta aatgtatctt tagatggaga agatgttaca aaagaagcaa
    78721 ttgataaatt tgtcaaagca gatataagta tgcaaaatat aaaggcaatt gattttaatg
    78781 ctgaatttgt aaaatctttg ggtgcaatac cttgtccata ccatagatac tactataaaa
    78841 ctaaagaaat gcttgaggat gagttgagag agtttaaaga gggaaaagct agaggtcaag
    78901 tagttaaaga attggaagaa caattatttg aattatataa ggatgaaaaa ttagatgtaa
    78961 aaccacctca acttgaaaaa agaggagggg cttactatag tgatgctgct tgtaatttaa
    79021 taagttctat atacaatgac aaaaaagata tccaagtagt taatacatta aataagggtt
    79081 ctataagaga ttttaaagat gaacaagcag tagaagtaag tagtgtaata acaaaaaatg
    79141 gtccaaaacc aataagtata ggatatttac cagattctgt gcatggatta gtaagtcaaa
    79201 tcaaatcatt tgaagtattg gcagctaaag cagctgtata tggcgattat gaatctgctc
    79261 ttttggcact ttgtataaac ccattgattc catctgatga cttagcaaaa actatattag
    79321 acgaaatgtt ggaagctcat aaagattatc ttcctagatt taatagatag ttgtctatag
    79381 aaagtaggtt atggtatgac taaaattata ataaatgcag atgattttgg atattgtgaa
    79441 gctgtgaact atggaataat ttcagctcat aataatggta tagttaggtc tacttctatg
    79501 atggcaaata tgcctggggt agaacatgga gtaggcttac ttaaagaaaa taaaacttta
    79561 aattgtggag ttcatatgac tttaagttgt ggtagaccat tattatctaa cttaaaaact
    79621 attgttgata aagacggttt ttttattagg aggattacag acgaaattat tgaaaaaatg
    79681 gattgtgatg agatttatag agaactttgt gctcaaatag atagggtaaa agggcttgga
    79741 atagatatat ctcatttaga tagtcaccat catatccata ctttagtaag tttaaagcca
    79801 gtaatagaga agattgtaac caagtataat cttccaataa gaggtggatt tgaatataat
    79861 ttagagtatt ctaaagtagt tcctttaata gatagttttt ataaggaaaa tgtaagtgaa
    79921 gaatatttta taaaaaatat agacgaaata atgaagtatg atgtagtgga tattatgtct
    79981 cacccagctt ttttagatga ttatatttta aattctacat cttatgctat tgatagaacg
    80041 aaagagcaca agatacttac atctaaaaaa gtcaaagaat ttttagaaaa gaatggatta
    80101 gtcatatcaa gctatagaga tatttaggag gtcgaataaa tggatgagaa gatgatagag
    80161 atatcttttg gaataattgg atttgcaggt gatgcgaaag ggctagcatt tgaagcaata
    80221 aaagaagcaa agagcgggaa tatagataaa gctagagaat gtattgaaaa gtcgaaagaa
    80281 tcaattcata aagctcataa attccaaaca gaattgatac aaaatgaagc taatggtaat
    80341 aaaactgaaa taagtgttat acttattcat gcacaagacc atttaatgac agctatgaat
    80401 tttcaacaat tagcagaaga atttatagat gtatatgaaa gattagaaag taagtaattt
    80461 aaaattatta ttaaaaaaga gtgtataaaa ataattttta taattattaa atgcactctt
    80521 tttttattta agtagagttt agcaatatag tattatgtta ttgatacttt agaatttaga
    80581 cttctcataa ttgtaaggta tactagtacc aacaactaca tcttcaattt tttcaacaat
    80641 aagccaatca tccatatttc cttgatggtc aaaatcaaga ggtgttattt cttcaacatt
    80701 ttcaaattca actaagcata aacatttttt atgccaacga actttttgct tttcagataa
    80761 atttaatttt ggttgatttt catttaaaat tttagaaatt tcatcttctg ttaatttaac
    80821 atagttttct acctttttaa cagtggcttt tgcagatatt tttgcagttc ctttttccat
    80881 aaaataaagt aactcatctt caaatactct actatgtggt atttttctac cagcagcacc
    80941 tctaataacc attgtttttg ttccatccaa gattttatct aagacctttt ctcctttttt
    81001 tcctgcatta tcacaatata ctaaatgtac catgttttga atcctcccta ctaaattcct
    81061 tatctataaa ttgcatttta tcattgttta actgaaaagt cattgtattt gtgtccaatt
    81121 cttaaaaaaa atactgttta atataagaaa accttatcta agctatatat aattaagatt
    81181 aattaggaat ttttatcctt ttacaaaata atattgacta aaaatagaaa aatgctataa
    81241 taaatatgtg cttatgataa tcaatatcat gtaagtataa taaacaagta ggagaagtaa
    81301 tcatgaaaaa aataaaatca cttgcaatat ttatatctat tataactacg ttagtacttg
    81361 ttacagcatg ttcagataag aatacagagg ataaagataa atcagagaca agagttgtac
    81421 aatctgttaa aggagaggtt aagataccat caaatcctaa aaaaatagtt gatatatcag
    81481 gaagtagtga agaactatta ttagcaggtt ataagcctgt tgcaacagca aatgtagact
    81541 catatgaaac tgataaatta ccaagttaca taagagaaga gcttaaaggt gtaaagatag
    81601 ttgggcactc aatgatggat actatggata tggaagctat attagaagta aatcctgatt
    81661 taataataat gagtcaaaga caagaaaaaa tatatgacca attaaaagaa atagctcctg
    81721 tagttatgat gaaagattat gcaaatgact ggagaagcaa gttaactgat gtttcaaagt
    81781 tatttgacaa agaagaagaa gataaaagtt ggctacagaa gtatgatgaa aaagctacaa
    81841 agttaggtaa ggaagttata gaaaagaatg gagaaaaaac ttatttacca gtgttggcaa
    81901 gttcaggtca atttatggta tttagtgatg gtggaatagg tactctaatc aatgatgata
    81961 tgaaattagc tagacctaag aatatgccaa aacaggatgg aataacacta ccaatggtaa
    82021 gtatggaagg tttaacagat atagatgcag accatatagt tgtaattgct acagaagcag
    82081 ataaaaaaga tttagaaaat agtgctatct gggctcaaat aagagcagta aaagatggta
    82141 atgtaacaat acttgatgct gctccatttt tcagtcaatc atacaatcca attggaaaag
    82201 agttattatt agaatcagtt aaaaatgaat taacaaagta attcttaact tagtaggtgt
    82261 gagataatga tattcatttt tcacacctat ttttaataaa tcttaaaatt aaaatactta
    82321 atagatgata ttatactgac gattaaaagg gaaaaggagg taggataatt gactaagctg
    82381 agtaaaaaaa atataactta tttgttgata ttagtaagta tagttgtact tgtatttgga
    82441 attgtattat ccataactat tggggcaaaa gatatgaatc tatcaactgt tatagatagt
    82501 ttgataaaaa tggaagatgg aataaatatg agaatagtaa aggatgttag acttccaagg
    82561 gcaatagcag ctgcacttgt tggagggttt ttagcagttt ctggggcaat aatgcaagga
    82621 ataacgagaa atcctatagc tgaaccatct gttataggaa ttacacaagg agctacattc
    82681 gcaatttcga tatcactggt acttcaaaaa aaacttcctc aacttataca tggaagtttt
    82741 agtgtaatga tgtttgcatt tattggagca agtataagtg gattatttat atattttata
    82801 agttctaaat caagaggaag agttaataat gtaaaattag ctcttgcagg agtcgctctt
    82861 ggtacattgc taatttcttt ggcatcagca atatctatgt attttaacct atctcaacaa
    82921 ttaagtttct ggatatcagg agggcttgta ggagtgaagt gggaaggtat aaaattgcta
    82981 tttgtagctg gtggtattgg atttatttta gctattatta tggctccaag aataactata
    83041 ttaagtcttg gagaagaagt ggcaatagga ttaggacaaa aaactaagtt tgttagattt
    83101 atatgtatag tacttgtaat attaatgact ggagcatctg tgtcagtgtc tggtaatatt
    83161 atttttatag gtcttatagt tcctcaaata gctaaaggaa tagtaggttc agattataag
    83221 tatataatac cgagctcttt agtattagga gctgtattgc ttgtatatac agatatatta
    83281 agtaggatga taaatcctcc atatgagaca ccagtaggtt ctataacagc acttatagga
    83341 gtgccaatat ttatatacct tgttagaaag ggggagaaat aggtactatg aaaaatatta
    83401 taaaaataaa ttctaaaaca ttacttgtta tatcaatttt aatggtgctt atttttatca
    83461 cttttttatt gagtttaaat atgggctcat tttctataga accaatggat gttataaaga
    83521 cttttttagg gcagggtcag cgtaatcatg aaattgcaat atttaaatta agattaccaa
    83581 gaatagtaat agcacttttg gttggttctg cattatcaac agcaggtgta atacttcaag
    83641 gagttacaaa gaatgattta gctgattctg gaattttggg aataaattca ggtgctgcat
    83701 tgtttgtagt agtttatata taccttatga atggtaatgt atatgaaggt atgagtaact
    83761 tgactgtatt tacaatgcct attgttgcac ttataggagc tttatttgga gcatttctta
    83821 tatattctct tgcatggaaa ggtggtataa attcgtcaag attactactt attggtatag
    83881 gtgtaaacat agcttttact tcaattttga cgatattcca attgaaattt acaacacaag
    83941 aatttaatag ggttatggca tggacatcag gaagcatttg gggaacaagt tggaaatatg
    84001 taatagctgt gcttccattt atagtgattt ttatgggtat aactatttat aaatctaaat
    84061 atatagatgt tttaaatttg ggtgatgaag tatctataag tcttggtgta gatgtagaaa
    84121 aacaaagaag aagacttata atatatgcag taattttatc tggtgtatcg acagcagtag
    84181 caggtagtat ttcattttta ggtttaatag ctccacatat aggtagaaaa ataataggac
    84241 caaagcataa aagattaata ccagtatcag cattgatagg aacattatta cttttaatag
    84301 cagatactat atcaagaaat cttctagcac ctatagaaat accagtaggt atagtagttt
    84361 ctataatagg agtgccgtac tttatttatt taatgttggt aaatgattag gaaagggtgt
    84421 tgttatgaac tgtataaata ctaagaatct aaatgtatca tatggaaaca cagatattat
    84481 aaaaaattta aatttaaata taccaaaagg aaaaataact accatcatag gggctaatgg
    84541 ttgtggaaaa tcgacaatac ttaaaactat aggtagaatt attagcccta atagtgggaa
    84601 aatatatata aatggagagg acatctacaa aaagaagtct aaagaaatag caaaagatat
    84661 ggctgtactt ccacaaagcc ctcaagctcc tagtggtctt accgtttcag aattaatttc
    84721 ttatggaaga tttcctcata aaagtggatt tggtaattct agtaaagaag acagagagat
    84781 tgtaaattgg agtttagaag ttactggaat tgcagaattt aaggatagaa atatggaaga
    84841 tttatcagga ggacaaagac aaagggcatg gatagctatg gctttggcac aagaaactga
    84901 tatattatta ctggatgaac ctacaacata tttagatttg gcacatcaat tggagatact
    84961 aaaattatta gatgaactaa ataaaaaaca aggaagaact attgtcatgg taatacatga
    85021 acttaataat gcagcaagat ttgctgacca catgataggt attaagaaag gtaaaatagt
    85081 ttgtgaaggt gatgcatatg atgttatgac gaaggataat ctaaaagaat tatttaatat
    85141 tgatgcagaa atagcaacag acccaagaac aaataaacca atttgtataa cttatgacat
    85201 ggtgtaaatg gtgacaacat ggagaatcaa gaagcattaa gacatgaaaa aatatggatt
    85261 ttattattta ggtattcgat acctgcaata atagcaatga tggtaacttc actttataat
    85321 gtagtagata gagcttttat tggttctatg gaaggtattg gctctatagc tattgcaggt
    85381 cttggtgtaa ctatgccagt atttactcta ataatagctt ttggaatgtt ggtatcagta
    85441 ggtgctagca caagattgtc tataaagtta ggggaaagaa atagagaaga agcagagaag
    85501 atattaggta atgctttgac attatctata attatctctt taattataac tatattagga
    85561 ttagttttct tagaagagat actttttatt ttgggtgcaa gtaaagattc aatattttat
    85621 gcaaaagact atatgagtgt catattggtt ggaagtatat ttaatttagt tgcattttct
    85681 cttaataatg caattagggc agaaggtaat ccaaaattag cagcaaggac aatgattgta
    85741 ggatgtgtat taaacctgat attggaccct atatttatat ttgtatttaa tttaggtata
    85801 aaaggagcag ctatagcaac agtactctgt caaatagtag tatttttttg ggtaatacat
    85861 tattttataa gagggaagtc caatttaaaa ctaaaaaaat gtaacttaaa attgaataaa
    85921 gatatagtaa agaaggtatt tgcaatagga atgacacctt ttgctatgga agtagctata
    85981 agtataactc acatgtttac aaataattct ctaaaggttt atggtggaga tttagctatt
    86041 ggagctatga cagcacttac atctatttta ttgatgttta tgatgcctgt gtttggattg
    86101 aatcaaggta tgcaaactat aatatcatac aattatggtg caaaacaata tgagcgtgca
    86161 aagaaaactt taatattatc tatcatagtg tcagttgtca tattgtcatt tggattttta
    86221 gtagtacaag ttttcccaga agtatttgta ggtatattta ataaagattc agatttgatg
    86281 gatatagcag taagaggtat aagcctaaat ctaattacat tgcctataat ggggatttct
    86341 attgtaggac ctgtgtattt tcagtgtata agtaaagtta aacattcaat gtttttaact
    86401 cttttaagac aatttatttt atttatacca ttaatcattg tacttccaat tcaatttaat
    86461 ttagatggtg tttggttggc acagccagtt gcagatttta ttgctatgat aatcgtatta
    86521 ttattcttaa aaagggaatt taaaacaagc cgagtgtaag aaatttagaa aataaaatta
    86581 aattggaaaa taaaaagact tgtctttata aaatgattaa aattatttta tagagacaag
    86641 ttttttattt tatttaaacg gaactatcca tactggtttc ttctagtttt tttccaaaag
    86701 tcattttctc aaatatttct tgaggtagag gttttgaaaa aacatatcct tgtatcatat
    86761 cacaattaat ttttttaaga taatccattt gcaagattgt ttcaacacct tctgatatac
    86821 tattcatatt taattttttt gccaaattaa ctatagattc tattacatga ttttcagcaa
    86881 tattatctgc attaggtgag ctaaaaaaag cacggtcaag ttttaatgtg tcaactttta
    86941 tttcttttaa catattgagt gaagagtatc cacttccaaa gtcatctagt gaacattgaa
    87001 aaccttcttc atggatttgg tctattatat taattaattt ttggagattt tcaaatacaa
    87061 gtgtttcagt taattcaatt tctaagagct tagcaggaat gttgtattta ttacgaagca
    87121 ttctgtattt ttctaaaaaa tctatattat ttaaattagc acgtgaaaaa ttaactgaaa
    87181 caggtattgg agtttctcca ttatctatcc attttcttat catactacat actttttcaa
    87241 aaacatagtg gtcgagtttt gtaataaaac catttttttc aaagaaagga ataaactcgt
    87301 ctggtggtat aaggccttta tttgggtctt gccatcttac taatgcttct gcacctacaa
    87361 tagtattatc tttgaggctt attttaggct gaagatatac tataaattcg ttattattta
    87421 atgcttcctc cattctattt tccatttctt tttctttaat catttttaaa cgctctaaat
    87481 cactataaaa aacacatgta taaagtggat gatttccaat gtttccttta ttattttttc
    87541 gtgcaacatt tgcacggtct tgaattgaaa ctaaaggtag atttgggtca tcaattttat
    87601 atacacctat agatagagct ataaaatatt tacattcttc atttttattg aaactattaa
    87661 tatcattggt gatattttct atgtacttaa gaatttcttt ttgaggaata gttttaataa
    87721 gtacattaaa ctggtcatta gaaactcgac aaagcaattc tcctggttct agataattac
    87781 taatgacatc atgtatgtac tttaatgttt tatctcctgc ttcactacca aaagaatcat
    87841 taataagttt gaatttctga atatttatag ataccaatga atagctttct gatgaagatg
    87901 actgtataat ttttttagct tcaatatcaa aacgtgcttt actgtaacct ttagttacag
    87961 agtctacata ggcgattttt ataagttgtg ccctgttttt ttgatttata aacataatca
    88021 atataataag caataaaaat agtataacaa ttaaaatatt gatgaagatt gcttttttta
    88081 gaagtaaatc aaactgtgca cttgctgttt ttgttggaac aattgaaagg aggtataagt
    88141 tttcttcttg taatggaaga tagtgcattg ccttagaaat accatcattt aatgtataat
    88201 acatagaacc agttttgttc ttttttaagt ttattttcat ttcttcaagt ttatttttat
    88261 caacctgttt tccttggtgt ttaatcatgt caaaaaaatt taccacattt tttggagcat
    88321 ttttgtttgc tgaacttaca ataaaatttc catcttggtc gataatttgg gaaaatcctt
    88381 caccatgaaa gctttctaca cttaataaat ttctcatagt atcaatagta ttccaagcag
    88441 caacagctcc aacaatttta tcgccattgt atagtggtac agaatataaa attccatatg
    88501 taccagattg tgatttaaca ggagtaattg tttctttgcc ttgaagggct tttctataaa
    88561 agtcttgtcc tttaacatta cctgtatctt taagggcact tacaaaattt ccattagaat
    88621 ctataatacc taaccaatca aattcaaata attttaattt ttttgataag tatctattga
    88681 cttctttttc actatcatta atctcaaaat aagtatctcc catagaatat agtgtcctaa
    88741 atatactttt acatttcaca ttaattgtag tggaaatacc ttgtgaaact tctacaagat
    88801 atcgttctgt ttcattatgt gttactttat ttagattcat aataaaagtg acacatgtta
    88861 atgacacgat taaaagaagt gtcaatagta aagtagctat tattttttta ttttttgtgt
    88921 tttcaaagtt cattcttttg tttcatcctc catcctttat ttgtttacaa ataagaattc
    88981 tctcagacat attataccaa atatcttttc atacaaagat attaaatttt atttatccca
    89041 ctttacattt agctttattt atgtcttctt agagattaat caagatacgt ttattgttta
    89101 aatcttattt tataatattt tgagtaaaag aggttaaaaa aagaactgtt ttcaaaataa
    89161 ttttgataca gttccttttc agtatagaaa atttttatag tattagaaag aacttttagc
    89221 ttatgtatct acataagtat tatatttata aaagatattt tgttacaatc taaaatgtag
    89281 atgaaattct gtaaatgcca aattctctat gaccaagact ttcagctttg gtatgtttac
    89341 cattacagac ttctattatt tcattaaata tttcttgacc tatatctgct attttagcac
    89401 catctgttat aattctacct gcatttatat cgatattgtc tggcattttg ttgtatgtat
    89461 cactattccc agtgatttta ataactggtg aaatagggct tcctgttgga gtacctctac
    89521 cagttgaaaa gattacaatt tgtgcaccac ctgctaccat accagtgata gaatctatat
    89581 cttgacctgg agtatccata aaatataatc cttttttatc aggaggtaca atatcagcat
    89641 attcaagagc acctttgaat ggcttattgc ctgccttata catacaccca agtgactttt
    89701 cttcaattgt tgaaagtcca ccagttttat tgcctggagt aggttgacca ctacgtaaat
    89761 cttctcccat agcaattgct cttttttcta catcacttac aaatttaaga aacttatctt
    89821 tcatttcttc atcttcaaaa cgggtagcca acaaatgttc tgcaccaatt acttctgtag
    89881 tttcacttag tatgctactt ccaccttcat ctacaagaag gtttgatgca acgcctattg
    89941 aagggtttga agctattcca cttgttgggt cagaaccacc acattcaagt gctaatgtaa
    90001 tttcactcat accaaattca actcttactt cttttgatac ttcacgagcc attttactta
    90061 ctatttcagc tcccttagaa actgttttta atgttccgcc acattcctgt ataacaacgt
    90121 aatctacctt ttttcctgtt gtagctattt cagatgctaa ttcttttgct tgaataccat
    90181 cacatccaag accaactact aaaactgcgg caacattagg attttttcca aatcctatta
    90241 atgtctttgc tgtaagatct atatcagaac caatttgaca acatccatgt tgatgaggga
    90301 ttgctattgc tccaggcaca agtgatgcta ttcttgtagc tgtctcactt gaacatacag
    90361 atgttggaat aattaaaaca tgattacgaa taccaaactg accattttct cttaaataac
    90421 ccattaattt cattttaact tcctcctatt ttttatctcc acgaccacgt gtactttcaa
    90481 tgttatgtac atgaacatag tcaccagttt ttatgtcaac tgttgcaatt cctataattt
    90541 caccgtattt gtatacaaca tcaccttttt ttacatcttc tgtagccatc ttatgaaaga
    90601 ctggtatatt atcatttact atgtaagatt ttccatcaat tgaaactttt tcacctttaa
    90661 ctaaaggtct gacacatgta actagattat ctttttcatt tgcatgaata attgcattca
    90721 ttaattttac ctccttaagt attaatttac ttcaatattt ttatttgttt taaaaataga
    90781 ttttttcttt ttttctcttt tatctagata ggaaactaac attggacaac atattgctgt
    90841 aacaataata gcagcagcaa cctgtactgt tgaaacagct gctattgtag ctagagtagg
    90901 gtctatagct gctaaagctt caggagtacc agctgcattt ccggctgttg tacctgtagc
    90961 tgcacctact gctggatgtt ctgcacgtat aagtttcatt acaaagtacc cacccatacc
    91021 tgttattata gtaactccta aaccaagtac aactccaggt agtccagcag ttataatttg
    91081 attaaagttt aatgctgctc ctaaaggaaa agcgaagaaa ggaattaata atgttgtacc
    91141 aggtgcaaga aattctcgta tatcttcatc aaggttacca agtataaatc caagtagtat
    91201 tggtataagt gctgcaaata gtgctattat aggtacattt gcaagtccag ttactccaaa
    91261 agcaagcatt gtaaaaaaag gaccatcatt tagagataag atagatattg ctccaacatc
    91321 tgtggagtct ccatattcac ctgcaagagc agcaaataga cctccatttg aattagtcat
    91381 ggtagcaact atggcaagag gtgtaatacc aattatacct ttaggtccaa taaatttact
    91441 aacaataata cctaatactg caccaattat aaattttgtt gctgtaagta ctataccttt
    91501 agtcaatggt ttaccagctt gtctgatatt gatttgagct ccattgcata gacaaaatag
    91561 agcaagtata ctcattgcac cagttttaaa taaggatgtt gtgaatcctc caatttcaag
    91621 agcttgtgga catactgtat taaatagtgc tcccaataac aagggacaga ccataagtcc
    91681 tcctggtatc tttttcactg attttaagat gtgcatctag tatcactccc tttcattttt
    91741 atataggtat tataaaagca attaatatgc caatatatag agtttgtatc tttagttatc
    91801 atgattattt ttaataatcg attgaaatgc tgtagattac ttataattaa aagtacgatt
    91861 gacgagaatt tcacttacaa agattctttt tatcatataa ttgtattttt tattgaaaac
    91921 aaataaacgt cttatgtatt aaaattaaac ataaaacgtt ttgatatata taggtatgta
    91981 ttatttttaa acatcatctt ctatttcttt taacttacgc catagagtgg agcgagttat
    92041 ctttagaatt tcacaggttt ttgtagtatt tctattattt tcgttataaa ttttatagat
    92101 atattttttt tctaaagttt ttaaatctaa attactgcat tcaaaatcac tattatcttt
    92161 atcaactata ttatttgtat attcattgct aaccttttct aataaaatcg agtcaaatga
    92221 ttccacaata acagcccttt ccatcatacc ttccaattcc cttatattac cttcatattc
    92281 tcgttcaaga agtgcctttt taatatttgg agtgataatt aaaggcgttt tattgtattt
    92341 gcttgaatat ttatgtataa aaaattctga taattttata atatcttctt ttctttcttt
    92401 taatgaagga agatataatg ttagtatatt tattctaaaa taaaggtctt gtctaaataa
    92461 accattgcgc atcatatttt ttaagtcttt atttgaagca cagataattt ttacatctat
    92521 aggaataact ttatcaccac caattctcat aacttgtttt tcctgcaata ccctcaataa
    92581 tcgaccttga agttcaagag ggatttcact tatttcatct aggaagattg taccttcatg
    92641 tgctagttcg aatacacctg ctttaccttt ttttctagca cctgtaaatg aaccttcctc
    92701 ataaccaaaa aactcactct ctattagaga tggaggtatg gcagcacaat ttacagcaac
    92761 aaaaggtgca tttttacgct tactatagtt atgtatactt tgacaaaata gttctttacc
    92821 aactccagat tctccagtaa taatcatagg agtatcataa caagcatatt tttttgcaag
    92881 ttctatacaa tcttttgtct tttcactagc atgaattata tcatcaaaag tgtatttagc
    92941 agatagacct ttgcttaata agctccttct tattgtatgt tcaaaagaac ttatctcctt
    93001 gctatcttga aatgttgaaa cagccccttg aatctgacca tcaacaatga ttggaactct
    93061 atttgtaata atttttgtac tccctatatt ttgtatttca ccaatttgag gttccatagt
    93121 ctcaactact tgtggaagaa ctgtgtttgg gataacatct acaacattat gataaagtgc
    93181 attttctttt tttataccaa atattttttc agcactctta ttaaataatg ttattttaaa
    93241 atttttatca actgttatta ttccatcatg tacaaaatct gtcatagctt ctatctgtaa
    93301 tcttcttttt tgttcatcct tggttgcttc gagtatttgt tctgcttgtt gaatggctct
    93361 aagtatgttt tcttctctag attcgattaa tattccctta caattaagtc tcttaacaat
    93421 atgtgcaaca gtggtatctc ctatatatgt gtttatacct ttctttctgt acttttctat
    93481 agcatcataa atttcttctt ctttttcaat ttcaatcata gttattttct tattcaatat
    93541 ttcttctata atatcgaaac cgtagatgac attattaaat ccaattattc caaatggttc
    93601 gttggaattt tttacctctt tgtaactttt tataatatca taagcatcta ccttaatttc
    93661 aactataggt atattgtaag tagcttttaa catattgtaa gttccacctc ggcttatgat
    93721 aattctagta ccatgattaa tacttttttc agcaagaggt ataccttctt ttagattgcc
    93781 aaatacaaca tcaatatttg tatatttgtt ttttttaact atgtgaattg atttttcata
    93841 ggttccttta gtgggagcaa taacgagtat ttccttcata ataattcctc catatttcat
    93901 caaataaact taaatattat cttatgacta aattttatca gttatatttt aacattttac
    93961 tttatattta attataaata aagtattttt aaaaggaaca ttataaaaga ctgtatcaaa
    94021 gttaactttt agaattataa agtttcatct ttttacagtc ttttaatata taatatctat
    94081 ttatcaaatc tatctacttt aaataatttc atatttttat tttgtttgcc aagatataat
    94141 tcacttaaca ttatacctcc taatattgca aagagtatac catttgctcc ataacctaag
    94201 caataccata gattgttatt tttattatct ggaccaataa aacctaaatt atctaaagtt
    94261 gaagcaaaac aaccacaata tttatattca acttcaatgt cttttatatc cttaaacata
    94321 cttttcaatc tttgttctaa aatatcatac ttttcattgg ctaaagtttc attttctata
    94381 tcatcaaaac ttacatcttc accacctata attaatcgat tgtctttagt agttcttaga
    94441 taattatatg ggtcacagtt atctctaatt aaaatgttgt ttttccaaga agctatgttt
    94501 ttaagtggtt tcgttgctat attaaaagta tttgacttag ttgcaaaatt tctttttgta
    94561 aataaacttg tattgtaccc tgtagctaca attaattttt taccatgaac tttgtatcca
    94621 tacgatactt cagcagtaac cttatcattt gataaatcta ctttttttac ttctgtattt
    94681 tcatatactt gaagaccatt ttttaaactt acatcaatca aatggtgact atatttatat
    94741 gggtctaatt ctcgtcctcc attctttgct atcaatcctg attttaaatc aaagttaaaa
    94801 gggtttgtgg attcatcaat atattcaaca tcaaatccat tttcttttct taagttatat
    94861 tcttctttga tagccttaac ttcaagtttg ttggcagtat aaagtagagt atctcttttg
    94921 gcataatcac acttatttcc atatagctct ataaatgtat ctaattcttc aagtgctgaa
    94981 gtacaaaggt tatatgcttt tagagcatct tttagagtca taacctctgt taaatctatc
    95041 aaattgtcat ctaattcata ctgtaataga gaagttgtca cgctagtgct tccatgtgca
    95101 atccttcctt tttcaagaat gacagacttt atattgtttt tagctagata gtaggcacaa
    95161 atacatccag ttacgccacc accaacaatt ataacatcag tatcaatatc atcagtaagg
    95221 tatggaaatt gtcttggaac tttattaata ttggtaaaaa taggatttcc ttttacatat
    95281 tcttgattca tatacttcac tcctaaaatt tattatgtat gagtgtatta ttttctatag
    95341 caaataatat tatacatttt aattatctag ttatatgggt atgtatagat aaaatgtgat
    95401 aacattttag tataaaacat atgtataata tattcataat taccttattt taagtaatat
    95461 agtatataat gtttgttaac aagtaaaaag attgaataaa agttaattaa aaatttaaag
    95521 taggaggtaa atttatgctt tcaaaaaata tagcttctaa ggtattagaa aaatgtctaa
    95581 ttactggagg agattttgct gagatttttg aagaagacac tataaataat tctattagtt
    95641 taatagataa taaagtagaa aatgcaatag gtggaagaaa ttatggtata ggaataagaa
    95701 tatttaaagg attgaaaagt atatatgctt atacaagtga caattcatta aatagtctgt
    95761 tggatgtagc atacaaagct gctgttgctt taggtaaatt ggaagatgga aaaagtgtaa
    95821 tattaaatga cagtattaaa ataaataaca tacataatat aaaaatttat ccaaattcaa
    95881 taggaaataa agataaagta agtgttatga aggttgcata taagagtgca aaagaattta
    95941 gtaatgatat atctcaagta agtgttagct atttggataa agaccaaaaa atattaattg
    96001 caaacactga aggtatatat gttgaggata gaagaataag aaccagacta gggataagtt
    96061 caattgcatc taagggtaat gaaaatcaaa ctggatttga aggtcctggg ggttgtaaag
    96121 ggtttgaaat atttgaagaa atagacccag aatattatgc aaaagaatca gctagagtag
    96181 cgcatactat gttgcatgct aaaaattgtc ctgcaggtaa tatgatggta gcaatagata
    96241 atggatttgg tggagtaata tttcatgaag cttgtggaca ttctttggaa gctacatcag
    96301 ttgcaaaggg gaactctgta tttgcagata aattaggtca acagattgct tccacaaaga
    96361 ttacagctat agatgatggt acaataccaa actactgggg ttctatgaat atagatgatg
    96421 aaggaaatcc aactcaaaag aatatactga ttgaaaatgg aattttgaag tcttatatga
    96481 tagataaact taatggtaga cgaatgggaa tgaatcctac aggaagttct cgtagacaga
    96541 actacaaatt tgcaccaact tcaaggatga ctaacactta tattgctgct ggagaagata
    96601 gaccagaaga tattataaag tcaataccag atggacttta tgctaaaaaa atgggtggtg
    96661 gttctgtaaa tcctgtaaca ggagagttta attttgcagt ttcagaaggt tacttaataa
    96721 aaaatggaga aatacaagag ccagttagag gtgctagttt aattggaaaa ggtagcgata
    96781 tacttatgaa tatagatatg gttgggaata acgtaaaaca agctcagggg atgtgtggct
    96841 catcatcagg aagtatacca actaatgtag gccaacctat gataagggta aaagaaatta
    96901 ctgtaggtgg aagataggag ggataattgt ggaacttaaa aattttaaag atatattatt
    96961 taaaaaggct ctaagtgaag gttttgaaga gtatgaaata tattattata caggagaaaa
    97021 tttaagtata aatatatatg aaggagaagt tgagaaatac aatcttgata aatcttttgg
    97081 tttatctttt agaggtaagg taaatggaaa gataggttat tcttatacag aaattttaga
    97141 tgataaagct gttgatatgt tgataaaaaa tgtaaaagat ggagtaaaca ctattgaaaa
    97201 tgaagatgta caatttatat atgaaggaga taagcactat aatgatgtaa agacttattc
    97261 taaagagcta gaaaatttag aggcagataa gttgattgat ttagctttag aaatggaaag
    97321 agagactaaa gcttattcag ataaggtggt aaatttatct aaatgtacta tatcttattc
    97381 tgtatcaagt aatggtatat ctaatactaa aggtcttaat ttaagcaata aaactaatat
    97441 gcttataggg tttgtagttc ctatcataga agacaatgga caaaaatatg atggaatagg
    97501 atataatata gcaaattcta ttgaagaaat aaaaccttgt gagatagcaa aacttggtgt
    97561 tgataatgct ttatctaaag taggtggtaa gagtattcca tcaggtaaat ataaaactat
    97621 attgcttaat gaagctatgg tatctttgct tagcacattt tctgggatat ttaatgcaga
    97681 ttcagctcaa aaaggattat ctttattgaa aaatagagaa ggagacatga tagcatctcc
    97741 tatagttaca atagttgatg acccactttt agaaaatggt atggcatcta ctccttttga
    97801 tgatgaagga gtagctacat ttaaaaaaga agttgtttta aatggaaagc ttataacttt
    97861 acttcataac ttaaaaacgg ctaataaggc aggtgtaaag actacaggaa atggaataaa
    97921 atcttcatat tcatcaccta taagcatata tccaactaat ttttatatag agaaaggaga
    97981 taaatcttta gatgaaatta taaaggatat agatgaagga ttgatggtta ctagctttgc
    98041 aggacttcat tcaggtgcaa attcagtaac aggagacttt tctttagctg ccaaaggctt
    98101 ttatatcaaa gaaggaaaaa aagtatttcc agtagagcaa attacagtag caggaaacta
    98161 ttttgacctg ttgaaagata ttgaagctat aggggaagat ttagaatttc caatgagtag
    98221 tataggttca ccttcagttg ttattaaaga gctatcagta gctggaaaag atgaataatt
    98281 aaaaagtaat tttgtggata aaaggtcatg ttaattttaa ttaatatggc tttttattat
    98341 ttaagtagat tttattatct aagttatatg aaaaagtcgt gatgcataca attaatatca
    98401 tgtattaaat ttaaaggagg agatattatg agtgatgcac attatgacat gcacaagcga
    98461 aatggatatt caagtgatga gccatatcca gagataaaag ttttaggtcc aaataagtat
    98521 tatgcagaat tattgatgga tgactatgcc ggagtatcaa gtgaatttac gagtgtaaat
    98581 caatatctat accataattt tgatttagat gaaacgcata gagagttaag tgaaatgtgg
    98641 ataaatatat ctataacaga aatgttacat atggagatat tagcaaagac tataagatta
    98701 ttgggtggaa atcctgtata tagaggttct acaagttctt gtggtgcata ttggaatggt
    98761 ggttttgtat gttatggaaa ttctatttgt aacagattaa aactagattt gcatttggag
    98821 catgtggcta ttaataatta ttataaagat ataagtctta ttgaagaccc ttatataaaa
    98881 gctatattaa atagaataat cctagatgaa aaacttcatg taagtttatt tgaaaaggca
    98941 atagagaagt attgtaaata aatttgaaaa tttagttatt catcatttta ttcatatata
    99001 taaaataacc atcatacatt ttcttgaagg gagttgctgt tgatatgtat tatgtatatg
    99061 tgaatgatat aaacatagca gtatatgatt tgaatccgag tgctaaaaaa actgtacttt
    99121 ttatacatgg ttggccctta ggacataaga tatttgaata tcaaacaaat atattgccaa
    99181 aactcggata tagaacagtt tcaattgatt taagaggttt tggtaaatct gatgcaacat
    99241 caggaggata tacttatagc caattagcag acgatattta taaggtggta catgctattg
    99301 gattaaaaga ctttaccctt gttggttttt caatgggagg agcaatcgta cttaggtata
    99361 tgagtttatt taatggatat ggagtgtcaa aattggtatt agctgcagct gctgcaccat
    99421 catttgtaca aagacctcca gaatttccat atggtatgac acgtgaagat gtaaataaat
    99481 tgatagcaca agcatgtact gatagaccag aaatggtaac cgattttggc gaaaaagtat
    99541 ttgcatcaaa tccaccagaa agctttagaa gatggtttaa tgatattggt tttagtgcat
    99601 caggtattgg cactattgga actgctgtat ctttaagaga tgaagaatta tttaatgatt
    99661 taaaatgtgt aagagtgcct actggtatat ttcatgggaa attagatgaa atatgtccgt
    99721 atgaatttgc agtttttatg aatgaaaaaa tagaagattc aattttatat acttttgagt
    99781 atagtggtca tgctatattc tatgatgagc ttaaactatt taatcagaaa tttttacagt
    99841 ttttagagga atagataaat tttattatca cttagaaaaa gagtaatatt atgtgaaaag
    99901 atttaagcta aattttatga tgttttttac actaaacaaa tagggtttta gaacttgtaa
    99961 ctaagttctg agaccctatt attatatatt cctaaaattt gttataattt attccttgtc
   100021 attcttaaaa atgtattaat atattaacga atttatatta aatttttgtt tatattccaa
   100081 taatgtcaaa ctaaccgttt tagaaattaa attatatgtg aaaaaagata gcttacatta
   100141 ttttattatg gaaaataata gtaaatggta caaaatttga tttttaagat ttaaaacttt
   100201 tttaaaaagt gctacaatag accaaattat tactttctat actcgttaaa caggaggtgg
   100261 gactatgaaa aaacataata ttttatttgt atcaactgat gacaaaataa atatagatat
   100321 atcaaaacaa ttggaaaata tatttggaga attttgtaat atagataact tggtatatgt
   100381 aaataggata aatattgaac tgtcaagcta tgagcttgta gtatgttcag acaatgatat
   100441 aaaggaatat atacataata atattgataa aaatatacct attgtaatag tacatcgtac
   100501 tataaatatt gaaaatataa atcagataat aagtatagaa aatgattctg atgttatggt
   100561 tatagatgct tataaagaat cagcagatga aactgccaag ataattagaa aactaggatt
   100621 aattcatatt aatctaatac catattatcc aggttgtgat aaatctaaat gtgagattgg
   100681 aattattacg ggaagcagaa acagcatacc acaaaacata aagcaaataa ttgacatagg
   100741 tgataaggta attgatataa atacagttat agaaatcttt acaaaactaa atatatctat
   100801 agataaacta catattatta aggaaaaata caatgaagat acagtaagtg gatataggta
   100861 ttatacaact atgaataaaa ccatgaagag ttttttggag attatagatg aaggtattgc
   100921 ttctgttgat aaattaggaa aatttatata ttgcaacaaa gtattttcta atctagttgg
   100981 aatagaccaa aatgaaatta tatctaataa ctttatggac ttatttagtg ataaggttgt
   101041 caaaaaaata ttttttcaag aagatgaagt taatgatgaa gtagtaaatt taaataacaa
   101101 aaaactgata attaataaag taaatgttta tgaaaataat gaacgaataa agagtattat
   101161 aagcattaaa aatataagtg caattcaagt gcttgaggac aagattcaaa ataaacttca
   101221 gtcaaaaggt tttgtatcaa agtatacatt tgagagtgtt gtaggtgaga gtaaaataat
   101281 aaaagaaaaa attaatattg caagaaaaat agcaactaca gacttttctg tgttgatact
   101341 tggagaaaat ggaacaggaa aagaaatatt tgcccaggca atacataatg aatctctaag
   101401 aaaaaacaag ccatttgttg cagttaatct atcttctttg tcagatacac ttattgaaag
   101461 tgaacttttt ggctatgatg aaggaagttt tacaggagca ataaaaggtg gaaaaatggg
   101521 tatatttgaa agagcacata caggaacaat ttttttagat gagataggtg atatatcttt
   101581 agatgtacag cagagattgc taagagtatt acaggaaaaa gaagttatga gaatgggcgg
   101641 aagtaaaatt ataccaatag atgttagaat tatagctgca acaaataaag atttaaagaa
   101701 aaaaattaaa gaaggttctt ttagagaaga tttgtattat aggattaatg tattacatat
   101761 agagatacct agacttagag aacgtaagga agatatacct cttataagta agtatttttt
   101821 agatgaaata aatagcaaca aatgttttac agaggaatca atgcaagctt taacattgta
   101881 tgaatggcca ggaaatgtaa gagagcttaa aaatttaatt tattatattg atactatagt
   101941 tgaggaagat agagtagatt acgagcattt gccagaacaa tttagatttg aaaaaaacaa
   102001 tactttagtt aatgaaaatt tcgattcaat tatcttagat ttcaaacaat ctaatttctt
   102061 tgaggaaagt atatgtatat tgaccagtat agaaacttgg aataataaaa atatattatt
   102121 aggaagaaat aaactgcaag agattttaaa agaaaaagat atagttttaa gtgtagacca
   102181 aattagaaaa agaatagata agttaaagag ccatggttta cttttatctg gagtaaaaaa
   102241 acaaggtagt tttataactg atgaaggaaa gaactttata tcatatgtaa aaattaaggg
   102301 agtgatatag gggactaaaa aggcaaaaca ccctaaaaaa atccctataa tggatagaaa
   102361 taaataaaat tatctttaag attaagataa ttcaaaaata ttgaataaat aatctaagaa
   102421 gattattttt aataaaacag tagtaaatat caaccttact actgttttta ttttttgcaa
   102481 gatttattat acaattaaaa tttatatttt ggcacatcaa ttgctacata ggaaaaatat
   102541 aaaaaattgt agaacttgga ggatgttgtt atgaagtcag cttacaaagg atatgaagcc
   102601 tatagttatt tagaaaaagg aaaagatatt tcatctattg agatgtgtaa aggagataaa
   102661 agagttaaag aataccttat tgaattagat gatattcaag aagctaaggt taaaagtata
   102721 gtggatgaaa aaatatttat atcattacat gaacatccag tattatttcc agaacaccta
   102781 gaaagagata tatttgaata taatagggaa ggtaaacaaa gatgtgctta tgaagcatta
   102841 tcaagaggat attatgatgc tatatttgat aatttaatgg atggtgtatg tacgataaca
   102901 tcaaattcag ggtggaaatg ggatgatata ttaatagatt taggcatgag actatgtgat
   102961 ttagctcatc aagattttgt tataaagtgc gaaaaagtag aagatatata cagagctaaa
   103021 agtgaaggta aagtagcatt gataccaaca ttagaaggtg cagcaccaat agaaaatgaa
   103081 cttgatagaa tagatatact gtatggattt ggtataaggt taatgggtat aacatatagc
   103141 gaatccaatg cattaggttc aggactaaaa gaagagaaag atggaggtct tacaaatttc
   103201 ggaaaaaaag ctgttgaaag aatgaataaa attggaatgg caatagattg ctctcacgtt
   103261 ggagttcaaa ctacacttga tgtaatagaa tatagcaagg acccaataat attaagccat
   103321 gttggggcaa gaagtttatg gaacacaaag agactaactc cagatgaagt attaatagca
   103381 tgtgctaaaa aaggaggggt tataggtgta gaagcagcac ctcatactac aataacagag
   103441 aagaataaag aacattcaat agagtcattt atggaacatt ttgagtatat taaaaattta
   103501 gtaggaatag accatgtagc ttttggacca gatacagtat atggcgacca tgtaaaatta
   103561 cactcagtac tagctgctaa atttgatata aaaaaaataa caggaacaaa agaacatccc
   103621 aaagtagaat atgtaaaagg tcttgagaac ccaacagaaa cttcaaaaaa cattgttaga
   103681 tatttagtaa agcatggata tagtgatgag gaaatagaga aggtactagg tggaaatata
   103741 cttagagtac taaaacaggt ttggaaatag atatattaga aatattaata aaatttgagg
   103801 atttataggg aggttttata aatggattat ttaaaattag ctaatcatcc acttttgtgg
   103861 atagcgagta cagtagcagt atcaatagta gtggtacaat cccttatatt tgctataaaa
   103921 tcttgcaaag ttggtaaaac aatgggaata acagataaac aaataaagtc agctgttaag
   103981 agtagtgcta tttcagctat tgggccttca atgactatat ttgctggaat ggtatcgctt
   104041 atagtaacta tgggtgggcc tatagcatgg atgagattat catttatagg ttctgtaatt
   104101 tttgaatcaa tgtcagccgg atttggaaca gatgcattag gtataacttt aggttctcct
   104161 gaaatgacaa aacttgcatt tacaaatgca gtttggacta tgatattagg ttctttagga
   104221 tggataatat tcacattact atttggacat aaactagata agataaccaa tgttatatct
   104281 agtggcaaga aatcatttat accaataata tctctaggag caatgttggg ttcatttgca
   104341 tatctaaatg ctgatagggt gttgcgtttt gataatggaa caattgcatg tataagtgga
   104401 atggctataa tggtaatatt gtgtatgctt gaaaagaaga aaaatataaa atggttaaga
   104461 gagtgggggc ttacaatatc aatgtttagc ggaatgataa tagcttcagt aatataaggg
   104521 gggagtttta tgagtaaagt aaaatctata tacaatgaag aatatttacc ttttaagata
   104581 aggtatggaa gactaacact atctttgggg attatagcag cattagttcc tggaataatt
   104641 ttgagctttg gatttggtat tatgcctcca atatcagcat tattagcaag tacaatggca
   104701 attgtcagta tgagtgctcc aaattacata atagaaccag tttcttattc accaatctta
   104761 ggtatcccag gaacttatat gagttttctt tctggtaata tatctaatat gaggctacca
   104821 tgttcaatag cagctcaaaa ggcagcagaa gttgaatcag gtacagaaga aggctcgata
   104881 atatctacaa taggaatagc tgtatctatt ttagtgaata taagtatact aactataggg
   104941 gtaattttag gaggaagcgt actatctaaa attccagctg aagtagtaga aaaattaaac
   105001 ttaattttac cagctctatt tgggtcagtt tttggacaag tatttttaca agataaaaaa
   105061 ttaggcctag tagcaattgt aatatctgta ttgactatta tattatcaaa gcaaggtata
   105121 ataccacagt ctctagtagt tcttatatgt gtatttggta ctatgttaat tgcaagagct
   105181 atgtataaag ataagcttag tgactaatta caatgaaata atagtgaatt ttcaaatatg
   105241 aaatataata atttgatttt caaatataaa aattacatta agattattta aaaattggtt
   105301 tttaaggagg gaatattttg atatatgatt taatcttaaa aaatggattt ataataggtg
   105361 gtacaggtaa tcctggtttt tatggagata tagcaattaa agacaactta atagcaaaaa
   105421 tagattctaa gataaatagc aatacaaata aagagattga ttgttgtgga aaagttataa
   105481 cacctggatt tattgaccct catgttcatg aagaaatagt agcaatacta gatggtaaat
   105541 ttgaaaagtt tctaaaacaa ggagttacaa caactataaa tggtaattgt ggacattcta
   105601 taacaccata ttcttctgaa aatgtatatg aatatatgta taaaaatggt ctattacttg
   105661 aagaagaaaa aaagtatctt atagataaaa ataaatgttg gaataatttt acagaatatt
   105721 gtgaccttat atctaaaagt ggtatttctg tcaatatggg attcttgttg gggcatggga
   105781 ctataagatg gagtgttatg ggaggttcta aggatagacc tcctacagaa aaggaaaaaa
   105841 atgaaattac agatataata aatgatggaa tgaaaagtgg tgcttttgga atatctactg
   105901 gattggcata tataccaagt aagtatgctg atattgatga acttgttgat attgcaaagc
   105961 agattaaaga gcatgatggt atatatacat ctcatattag agattacata ggaagatata
   106021 atgcagtaaa agaagccata gaggttgggc aaaaaagtgg tgctagggtt caagtatctc
   106081 acttaagtcc agttgaaata gaagcatttg atgagatatt aaaggctagg tataatggtg
   106141 ttgaaataat ggttgacaca gtaccaagaa gttctgggca ttgtatgaag aaaaaaagag
   106201 taattcaatt tattatggca atatcatcta gtctatttga attaggtata aatggggtaa
   106261 tggatgctct tagaaatgaa gagggaagaa cacttatatt aaaagaagca tttatacttg
   106321 gagatagggg gagtataata cttctaaaca ctaaagatat taatatggaa aagaaatcaa
   106381 tacgtgaaat agctacacaa aaaggaatag atgaagacaa gttattactc gatttacttt
   106441 tagatggaga cgaagaactt atcttttgtt taggaggaat gtatagagct gatttcccag
   106501 ataaactaca tgatgataaa ataattgata atccatttgt tatggttggt tcagattgtt
   106561 tgtttagtgt gggtggggat atgtcatggt ttgaactaca aagaagagga gcttttccaa
   106621 ttttcttcaa tatgtacaga aagtctggtg ttagattgga agagattgta agaagagtta
   106681 cttcattacc agctaggcaa tttaaaataa aaaatagagg tattcttaaa gaagggttga
   106741 tagctgatat agcagttatt gacatggata actatagtta ttctaggtca gaagatattg
   106801 atttttcaaa gccacaatta ctagccaatg gtgttgaata tgtaattgta aatggaaaaa
   106861 ttgcgttgga aaatggagag attactgaga ataaatgtgg tgaagtatta aaaagataag
   106921 gttttaaaaa taataggatt tagaaagtaa ataaatatct aagtaagtaa agacgaagtt
   106981 tgagatttat ttaaataaaa atcatgcttc gtttttattt atagtattaa tatttatttt
   107041 aataatcaga taatatttat actttatagt gaaaaaatta atatttttaa tcattaaatt
   107101 atgtaaagaa ttggataaat agataactaa atcatttgag aaaagatttt tagaaataaa
   107161 aaatgataac taaataaccc ctttaatatt tatcaaatag ccagtcataa atattaaaag
   107221 gggttattta gtttataaaa attatttgta ataaagtagg atatattaat aaatatgata
   107281 gaatatatat tataaattaa atttattaat ataagggggg aactttgagt tcttatagat
   107341 ttcttacatt ataagaacct atactttatg cgggagttat taaaagtaac acttatagta
   107401 atgcttgttt tgataaatgc tttttttgta gcttgtgaat ttgctatggt aaaacttcgt
   107461 agttctagga ttgataccat gataactgaa ggtaataata atgcaaaaag atgtaaaata
   107521 ataaaagaca acttaaattc ctatctttct gcatgtcaac ttggtataac actttgttca
   107581 cttgcattag gttggatggg agaatctaca gttaaagagt taattttacc aatagtaagt
   107641 gtatttaatc ttagtgaaag tattatatat actatatcaa tttccatttc ttttttaatt
   107701 ataacaatga tggaggttgt aataggagag ttggttccta aagctttagc actttataat
   107761 acagaaaaaa taatgttgag tacatctttt ttattagtag gattttataa gttaacatat
   107821 ccaataatat attttttcaa cttaagtact gatttatttc ttaaaccatt tggatattca
   107881 caagctgatg aggttgatga accccataca ggtgacgaaa taagactatt agtagaagaa
   107941 agttataaaa gtggacttat agatgaatca gagcaaagat tagttgataa tatttttgaa
   108001 tttgaagaaa agaaaattag agagataatg gttcctagaa ctgatatggt atgtatatat
   108061 gaaagtgatt ctgaagaaaa gatattagct attttaaaag aggaaggtgt tacaagatat
   108121 ccagtatgca gaaaaaataa agatgatata ttaggttttg tacatataag agatttatac
   108181 aatcaaaaga taaatgaaaa taaaattgaa ctagaagaaa tattgagaga tattatatat
   108241 atatctgaaa atttaactat agataaagct cttgagagaa taagaaagga aaaacttcaa
   108301 cttgctatag ttgtggatga atatggtggt acatctggtg tggtaacaat agaagatata
   108361 ctagaagaga tagttggaga aattcaagat gaatttgata aagaagagac tcatattaag
   108421 aaaataggag caggttctta cttagttgat ggaactgaat ctatcaacta tattaataag
   108481 tattttggac ttgatataga gcatgatgga tttgacagta taggtggatg gatatcctat
   108541 atgcttggtt caaatattaa agtaaaccaa agtgtaactt ttgaaaatta caattttaca
   108601 atattagaat tggataaatt gagggttgta aaattaatca taaaaagtgt aatataatta
   108661 tatcaaaaaa tattggagga attgtatgca aaaagtagaa ggaattattt ttgacatgga
   108721 tggagttttg tttgactcag agagaatttc tcttgaattt tggatggaga cgtttgaaaa
   108781 gtatggatat actatgacta aggaaattta tacatcagtt atgggaagaa atcgtaaagg
   108841 aataatagaa ggtcttacag atatatacga tagttctgta ccaataatag atttatatga
   108901 cgaaaaaact aaaaatatga ttgaatttat ggagaggaag ggagctccta tcaaattagg
   108961 agtcaatgaa ctaatctctt ttttaaagga aaatggatac aaaatggctg ttgcaacatc
   109021 aacaaagaga gaaagagcag taaagagatt agctaaggca aatttaaaag attactttga
   109081 tgctatagtt tgtggagatg atgttgtaaa ctcgaaacca aaccctgaga tattcctaaa
   109141 agcagctaaa aagataaatg ttaatcccaa aaattgtatt gtcatagagg attcgcctat
   109201 gggagttgaa gctgcataca atggtggtat aagatgtata aatgtaccag atttaaaaga
   109261 gccagatgag caaattaaat cacaatcaca taagatactt gaaaatttac tggaagttag
   109321 agaataccta aaaagtttga actctaaaga atgtcacaat ctataatata aagtattaat
   109381 taaataaatt aaatataatt tctttgattt agaataaatc catgaatatg agaaaaaata
   109441 agtataaaca gctaggagga atattcatgg ataatgtagg ttctctatta tattttttat
   109501 ttaatttttt cttgtatggt tttataggtt ggataattga aaacttatat agttattata
   109561 aacaagggca ttttcaagaa gatggatttt taaatggtcc atttaaacca atgtatgcaa
   109621 ttgcaatgtc gattataata gaggtctcaa gatttattga ccccaataat atatttgcat
   109681 tgcttttatt atgtctagta gtgccgacag tagtagaata tattacagga atacttatga
   109741 aagtatattt taataaggtc tattgggatt attcagatta taagtacaat agtagaggaa
   109801 tagtatgtct aaagttttct gtatattgga ctatattgac atttattgga gttagatatt
   109861 ttcaagtgta tatagttgat agtttatata gtcttataat accttattgg ccaattgttg
   109921 taactatact tttagtaaca ttgtttgtgg atgaaattct tactataaaa tttttgaggg
   109981 gaaataaaga tatagtagaa agataacttt aaaaatataa gttgaattta gattgtagtt
   110041 aaaagaaagc tatgttaaaa tggtgaaatc atttaaaatt agctttcttt tttatttgat
   110101 aaaattattt tataatatat ctataagttg taaagttttt tattaaaatt aaaataaagt
   110161 gtagaaaatg gggagtgatt tatgatgggt aaaataagat ttggtattgt tgggacaagc
   110221 aatatagcaa atatatttct aagagctgca tctagggtta aagactttga attggttgct
   110281 gtatattcta gaaatttaga aaaagctaga gaatttggaa ctctatatgg agcaagtatt
   110341 ttttttgatg atttagaaca aatggctaaa tcgaaggata tagatgctgt atacatagct
   110401 tctccaaatg cattacattc aaagcaaacc attacttgtt taaaacacaa aaaacatgtg
   110461 ctttgtgaaa agtcgttagc atctaattta aaagaagtca aatcaatgat aaaaacagcc
   110521 aaagataata atgtattgct tatggaagct atgagaatta catgtgttcc aaactttaaa
   110581 gcagttaaag aaaacttgta taaaataggg aaaataagaa gattttttgg aagctattgt
   110641 cagtattctt caagatatga taaatataaa aatggaatta tagaaaatgc ctttaaaaaa
   110701 gagttgtcaa atggagcttt aatggatata ggagtgtatt gtatacatcc tatggtgaat
   110761 ttatttggtg ctccaaaaac tgtaaaggca gtttctcata tacttcaaac tggagtggat
   110821 ggagaaggtt cagctgtatt tcaatatgat gatatggatg cgataattca gtattctaaa
   110881 gtagctgatt cttatatacc atctgaaata cagggagaag agggaagtat aataattgaa
   110941 aagcttaatc tatttgaaaa agctattatt aaatacagag atggaagaga agaagatata
   111001 tctattccaa aagaaaaagc agaggaaaag cctagagaaa tagaaggaat gtattatgaa
   111061 cttgtagagt ttataagtct tataaataac aataaaatag aatcaaatat aaattctcat
   111121 gaaaattcaa taatagtaat ggaaattatg gatgagataa gaagacaaag tagtattgtt
   111181 tatccagctg atagtatata gtttatatta gaaaatataa gaatttataa tatatattct
   111241 taaataaatt taattttaag ttaaggtaag gtgtatactt aaccaagtgt taaaatgtca
   111301 aattataagt atgaaaaagt gctaaaaaga aatttatttt tggcgttttt ttcttttttt
   111361 ttacaatttt gtaacagtat caaaaaaagt tttgacgatt ttattacaat tttgtagttg
   111421 aatatacata tcaaaactaa tatgattaat aataacataa atttgaatat ttttagagga
   111481 gagaaaatat gagaaaattg atatacttta ttacaccttt tataataggc gtagtatttt
   111541 tatttgggtt agacaaattt cttgattcta aaactgatga acttttgagg gagaaaaatc
   111601 tacttccaat tatggatgat actttaagtg atataaaaga taaaggggtt acagcaaata
   111661 atcatttttt aagagaaaag gatattatga ttttggggtc atcagaactt agtaattcaa
   111721 caaaacaaca tccaaagtac tattttaata caaataggag taaaaataag gtgtttgcaa
   111781 ttgggagagc ctatactcag acattacaag atgctgcaat attaggaagt atgaatccta
   111841 atattgataa taagaaagtt gtcttgttaa tttctatgca atggtttatg gaaaaagacg
   111901 gagtcacaag tcaccattat cagtctagat tttcaccaat acaattttat cgatttttag
   111961 ataatccaaa aatatctaaa caaaataaga ttgaatatgc aaaaaaatca agtaagttat
   112021 tatggggaag tgacgagtat aaagcagaag cattatatgc taagctatat gagcctaaga
   112081 catttcctga aaaagctgaa aaggtgcttt tagagcctta ttttcaagga agaaagtatt
   112141 gtatagctct taaagaaaag ggtattcttt ataaacgatt aattaagcta gataaaaaga
   112201 gagcaacaaa aagaaaaaga ccaataaatt ggtcatatga gagaaagaaa gccattgaag
   112261 atgctaaaaa aagagttggt aaaaatccac taaatattga taagtactat tataaagagc
   112321 attttaaaga tggtatagag caatacaaag gaagagacaa agatgtaaat ttattaacat
   112381 ctaaggagtt tgaaaattat aaactgatgc taaatgtatg tactgactta ggtattaagc
   112441 cagttgttgt attaatccca agtatggata aattctataa tttgactggt atttctgaaa
   112501 aagaaagaaa tcagtactac gataaagcac aaaaaatagc agaaagcaaa ggctttgaag
   112561 ttctaaacct taaagataaa gggtcagata agtattattt aagggatgta atgcatcttg
   112621 gtacgaaagg atgggtagat gtctgtgaga gattatttaa aatatttaaa gaacaataaa
   112681 gatttgttaa ctagtatttg tttaactatg gttataatat tggcgatagg agttctaaca
   112741 tttactccat ttaaagaatt gccatttatt tataacggat tttaaaggag cattcttata
   112801 tgaaaattat tgaaggaata aaaaaatatt caaatacaga tagaacagca ctaatgtgta
   112861 atggagataa actttcttat aaagatttaa atgaatattc agatgctata tcagtatttc
   112921 taaaggatgt atacaaagaa gaagatactc ctatagtaat ttatggaaat aaagaaaaca
   112981 tgataatggc atgtatgata ggagctttaa aatcaggtag agcgtatgtg cctcttgata
   113041 ttagttttcc aattgataga gtttttgaag tcactaaaga gataaaacca aaggtattat
   113101 ttaactttag tgatgaaaga aattttggag atatcaatgt aattgatatg gataaattaa
   113161 attatataat aaatgaatat cagggaaaat cattagataa agaaaactgg gtaaaagatg
   113221 atgaaaatgc atatatacta tttacttcag gaagtacagg aaagccaaaa ggggttcaaa
   113281 taagttcaaa caatttagat agcttttcag attggataag cccatatcta aacatagatg
   113341 gaagtgaaaa agtaataatg aaccaaccag catattcttt tgacctttca gttacaacaa
   113401 tataccctgg tcttatccat ggagctacat tattttcaat atcaaaagat gtattagcag
   113461 actataaaga actatttaga caatttagta tctcagatat agctgtatgg gtgtcaactc
   113521 cttcttttgc aggagtatgt ataactgaaa aagagtttaa tagtaaaatg cttcctaacc
   113581 ttgagtcaat gatattcata ggagaagcac tttcaaaaaa tcttactaaa gaacttatga
   113641 gtagatttcc aaacacaaga attatcaatg gatatggacc aaccgaggct actgttggtg
   113701 taagtgtaaa tgatatgact caaaaggcta tagatgatga aaaaagtctt ccagttggat
   113761 accctatgtc aaattgtaaa ataaaaatac ttgatgaaga tggaaatgag cttaaagaaa
   113821 atgaaaaggg tgaaattata ataattggac catcagtttc taaaggttac ttcaataata
   113881 aagaaaagac tgatgaagta tttttctatg atgaaataga tggtgtaaag tggagagctt
   113941 ataaaactgg agatatggga tatcttttag atggaaatat atattattgt ggaagaaaag
   114001 attttcagat taaattaaat ggatttagaa ttgaaataga agatatagaa aataatttga
   114061 gaaaagtaca taatgtaaaa aatgcagtag ttttaccagt atataaggac gaaaaaattg
   114121 cttacttaaa aggtattgtt gagttaaatg aaaagaatga tttaagtaat attaaaaatg
   114181 ggatgataat aaaaaaggaa cttggaaagt atataccttc atatatgatt ccaagaaata
   114241 tttcaataat ctctgagttt ccaacaaata tcaatggtaa aatagacaga aagaaactta
   114301 tggaggaaat ttaatgattt tttcacaata tggagattat ttttatcttt atatattatt
   114361 attaacatca ataccagcag taattttagg tctaatgggc aagaatataa agtattatgg
   114421 tatgttggca agtttgttta tgatattctt aatagtagga attgatgtcc aactaaagta
   114481 cttggtaata tttattattc ttgaagtaat aatagttaaa ggatatgaat atgtaagaag
   114541 aaaaactaaa aataaataca tatattgggg attcttgttt gcatcaatgc tcccaataat
   114601 aataaataaa atatctccag ttacttcttt tggaataatt ggatttattg gaatatcata
   114661 cttaaatttc agaactatac aaatggttat tgaaatttat gatggagcta taaaagaagt
   114721 taaaatttca aagatgcttt attttatgtt attttttcca actttaagtt ctggacctat
   114781 tgataggtca agaagatttg aacaggactt ggaaaaacaa atctctagaa aagagtatat
   114841 agaagaatac ttactgccag gaataaaaaa tattgtcatg ggagtaggtt ataagtttgt
   114901 tatagcattt ttgataaata catattgggt atcaagaata cctaaagata tgagttttat
   114961 aaatatttta agttatatgt acgcatatag tttatacctg ttctttgatt ttgcaggata
   115021 tagtttattt gctataggta cagggtacat atttggaata caagtaccta taaactttga
   115081 caagccattt atcagcaaag atatgaaaga gttctggaca agatggcata taagtctttc
   115141 aagatggttt ggagattata ttttttctag atttgttatg tcatcaatga gaaagaaaag
   115201 atttaagaaa aggactactg ctgctcatgt ggctcagatg ataaccatga taacaatggg
   115261 attttggcat ggtctaactt ggtattatgt tgcttatggt gtataccaag gattggcact
   115321 tgtgttaact gatatatatc agcgaaaatc taaattttat aaaaaacata aaaaagataa
   115381 atggttcgag agagtgcaga ttttcattac attccatatt gtgtgttttg gattgctgat
   115441 attctcaggt tatttagctt aattttaagg aggaaaattt atgcaagaaa cagttataga
   115501 aatatttgaa gatgtattag ggactgatga gataagagaa gatttagacc ttaatttatt
   115561 tgagacggaa cttttagact cattagctat aattgaagtt ttacttgaaa ttgaaaatag
   115621 attagggata gagcttcaac caactgattt agagagaaaa gatatgtcta ctgtaaataa
   115681 tttagtaaaa ttcttggaga caagaaaata aattagtagt tagactttaa atattaattt
   115741 ataaaataaa attttacaaa gtccatctga tactaatatt tgatggactt ttttaatgat
   115801 aatacaaagt cttttgaaaa taactttaat taagtaaaaa atttaataca taaatatgat
   115861 ttcataaatc ttaggtatca ttgtaatatt ataggagaaa aatatattat atttaggagg
   115921 aaaatgaatg tctttaaatt cgatagatag aaaaaaagaa atacttaaat tactagattc
   115981 aaaagggaag gtaaacacta aagaattagt aaaaagatta gaagtatcat cagaaacaat
   116041 aagaagatat ttggaagaat tagaaaatga aaataaatta aaaaaggtat atggtggtgc
   116101 tataaaaata aattatggag tgattgaacc agaacatata aagagaaaca atactaacct
   116161 agataaaaag caaagtattg ctaaatatgc agctaagttt gtaaatgaca atgagtcaat
   116221 tatcatagat gaaggtacta ccaatttgca gattgtagat tatataatat ctgtaaacaa
   116281 tttaaaaatt attacaaact cttatccagt tctgtcaaaa ttgatttctt acgagaatca
   116341 aggtttgttt gatggagaag ttatatttat tggtggaaaa gtaaatttaa aacaccaaag
   116401 gacagcatgt ccaatatcaa tagatgctat gaaaaactta tacgtagata aagcttttat
   116461 atctagtgaa ggtatacttg atagttttgg aattagtagt atagaccaaa ataaggcatt
   116521 gttgtcaaaa gcatatataa aaaattcaaa ggaaagtttt gtattatgtg atagttctaa
   116581 aataggaatt gctagtatgt ataagatagc agatttagag gaattagatt ttgttatttg
   116641 tgatgtagaa cctccgaatg aatggaagga taagttaaat gagataaata taacatggat
   116701 agaagcaaaa taaaccctta gttttatata atacaactat gctcaataca actaaattca
   116761 taaaatatag tcagttcata gattaagatt agaaagtaaa ttttagattg taacttagga
   116821 ttttatggga atatgataca aaataaaaac aactaaatac aactaaaaac agaaatttaa
   116881 tatatattta acctaaatat gatgtataat taacatatga aagttatact ttaaaagtaa
   116941 aaacaattaa aaacacaaaa attctaaaaa gaatctatgg aggctataaa tatgaaaaag
   117001 atatatggag aaaaaataaa agcagtagta ttcgattggg ctggaacaac agtagattat
   117061 ggatgttttg cacctcttaa tgtatttata gagatattta aaagaagagg tatagatgta
   117121 acaatggaag aagcaagaaa acctatggga aaattaaaaa tagaccatat aagagaaatg
   117181 tgtgaaatgg atagaataaa gaatatttgg tcagataagt ttggtaaagt gccaactgaa
   117241 gatgatgtaa atgaacttta tgctgaattt gagccaatgt tatttgagac attagaagag
   117301 tatacgactc caattcctca tgttgttgaa actattgaaa agttgagaaa aaatggatta
   117361 aaaataggtt ctacaacggg ttatacaaga gagatgatga acatagttga accaaatgca
   117421 gcaaagaaag gatactcacc cgattttctt gtaacaccat cagaagtatc tcaaggaaga
   117481 ccatatcctt ggatgtgcta taaaaatgct gaagctttag gggtgtctcc tatgagttct
   117541 atggtaaaag taggagatac aataagtgat gtaaaagaag gcgtaaatgc aggaatgtgg
   117601 agtgttgctg taataaaagg ttcaagtgag ttagggctta cacaagaaga agtagagaac
   117661 atggataagg aagaattaaa agctaagatg agtatagttt caaagaaatt taaagaggca
   117721 ggagcacatt ttgtaataga aactatggct gagttagaag atatactaat aaaaatagag
   117781 aatgaaacta ttaaatcaga ttttgtacca gaaaatgatt acatattact tacaccagga
   117841 ccactatcta ctactaaatc agtaagagca tctatgttaa aagattggtg tacttgggat
   117901 gttgaatata ataacctagt acaagatgtt agaagaagat tagtatctct agcaacccaa
   117961 aatacagata aatacacaag tgtattaatg caaggaagtg ggactttttc agtagaagct
   118021 ataataggaa gtactatttc aaaagatggt aaacttcttg taattgcaaa tggagcatac
   118081 ggaaagagaa tgaaagatat atgtaattat cttaacatac aatttgttga ctgcactttt
   118141 aaagatatag aagcagtgga tttaaatgta gtagaaaatt tattaaaaga aaacaaggat
   118201 ataacacata tatcaatggt tcactgtgaa actactacag gtagattaaa tcctatacaa
   118261 gaggtaggga aacttgcaaa agaatataac aaaatttata tagttgatgc tatgagtagt
   118321 tttgggggaa ttgagattga tgttgaagat tttaatatag attttttagt tagtagttct
   118381 aacaaatgta tacaaggagt acctggattt ggttttataa tagctaataa agagaaatta
   118441 tctaaatgta aaggtatagc taaatcatta tctcttgatg tgtatgcgca atgggagact
   118501 atggagaaaa ataatggtaa atggagattt acctcaccta cacatgttgt aagagctttt
   118561 tatcaagctt tattagaatt ggaagaagaa ggttctgttg aaaaaagata tgctagatat
   118621 aaagaaaatc agtttacaat agcttcaaga ttaaaatctt taggatttga tactttggtg
   118681 aatgataatg ctcaatcacc tgtaataaca actttcttat atcctaaaaa tgcaaagttt
   118741 gaatttatgg aattttatac ttatttaaaa gataatggat ttgttatcta tccaggtaaa
   118801 ttgactgata tagatacatt tagaataggt agcataggtg aagtttatcc aactgatatg
   118861 gagagactag ctgatgtaat agaaaaattt atcaatagat agaagaagat agtgtcttgt
   118921 aggttacaga atagtgtgga aagcccactc ctttaggtgt gtgagatgat agcacaaaca
   118981 gtgtataaaa agtattgaca gtagaattta aagacttata ttttagaagt ttatacaatg
   119041 aatatataaa gattgagtaa tagggatata ttttatttaa accacaatct aagaaattag
   119101 atttatagtt taaataaagc atttccctat tatttttatg cctatttata gaaaataaat
   119161 gatagttgct ttattaagta ttattatcat acttgacaac gtaacaatgt tttgttatat
   119221 taggtttaaa gataaaatac ggtaaagaaa ggatatcatt atgaatgaag aattaagttc
   119281 actaagattt gatggtgttg gaaaatgtaa aggtggaatt tttgataatg ttaatatatc
   119341 aggaacaggc aaaatagaag gaagtgtaaa atgtagtaaa tttgattctt ctggttcagc
   119401 aaaagttttt gggacaacag aatgtaatga attttttaca tctggtttaa gtaaagtaga
   119461 aggaaacata ctggcatcaa aagttgagat atcgggactt cttagatgta caggaaatat
   119521 taattctgtt gaaattgata ctagtggaat tattaatgta gaaggaagta taaaatctga
   119581 tattattcat ggagaaggat atttaaaagc aaatgagaat atagattgtg aaaatatcaa
   119641 tttaagtgga atcttggagt gtaatggatt tttgaattgt gaagaagtta atattagttt
   119701 aaatggaact tctaatttta atgaagttgg agcaagtagt ataaatataa aaaaggggca
   119761 tggagattat tgcaaaaaat ttcgtatgct agaaaaaaag catatcaact ttgaaaaagt
   119821 gtcttcaaat ttgataagtt taggtctatt atcaattaga aagtatacag aaagtaaatt
   119881 gtttgcaaat attatagaag cagatgaaat aagcttagaa aatagtgaag tcaaagttgt
   119941 aagaggaaaa aatattaaaa taggcaaggg atgttatgta gaaagtttgg agtacagtga
   120001 aagtatagag attgatgaag attcaatagt tggtgaaata aagaatataa gtgaaaaaat
   120061 aaaattaaag aaggataagt aataagtagg tttaaaaata aaatttaaaa tgaggtaata
   120121 atatggaaaa agggtctata aaaatacttg gagaaggctt tataagtagt ggtgagtatg
   120181 aaaatataaa aatcgtggga agctctaaat caaaaggaaa tgttaaaagt aacgaagtaa
   120241 aagttcttgg agatgcaaaa tttgaaggtg acatcaatat aaaaaatttt gttgttaacg
   120301 gaaaaactat tataagaggc aatcttaaag cagagcgtgt aagagtgaat ggtcaactta
   120361 atatagaggg agatgcagaa atagatgact tagttataac tgctcaacag aaagtggatg
   120421 gcaaacttaa atgtaataat gttacaataa gaggacaatt aagtgtaaat gaaaatatgt
   120481 atgcacaaga tataaaaatc tatggcgaaa tgagaaataa atcaaatata gaatgtgaag
   120541 atataaaggt atatggagca ataaaatgtg atggtctttt aaatggagaa aatatatatg
   120601 tttattcagg aggaggttct tattgtaaag agataggggc aactaatata aaaataggaa
   120661 gaagcaaaga tgctttttca ttaattaaaa tcattaatat tttttcagga aagtttaaat
   120721 gtaatttgat agagggagat actattgaat tagaagatgc atcaataaaa gatatacgag
   120781 gtaaaagcat atctctagta gataattgtg atgttggaaa tattgaatac agtgataatt
   120841 tatcagtttc agataattct actgtaaaaa acagtataaa ggtgggctag aaaagttatg
   120901 gaagaattaa tatctaagaa agatttattg agcaaaacta atatatctta tggtcaatta
   120961 tatagatgga aaagaaaaaa cataatacca gaggaatggt ttataaaaaa atcagctttt
   121021 acaggtcaag agactttctt tccaagagat aaaatactag aaagaataga gttaatattg
   121081 tctatgaaag aagagacttc tcttgatgat attgcaaata tgtttatgaa aaaggatatt
   121141 aataaagaat ttgacattga ttttattctc agtaaagaag ttatatgtaa ttataccaaa
   121201 gaaatatttc aaaatttata taaaaaagag caaagcatag gtaaaaaaga actgcttata
   121261 ttgagcataa tagagaagtt tttagttaaa tctataataa cttttgaaga gttaaaattg
   121321 atagtgaata taatagaaga aaattttaca tcaatataca atgaaagtgg aaagatttat
   121381 ttgtttagaa agtttggagt tccattcgta gtaggatgtc tggattataa aaaagtaaag
   121441 tttgaaaaag atgtagttaa gattattgag attgacttga caaaagaaat aaatgatata
   121501 agtataaagt tatcatagtg tattatctta tataatgtat tataataata tacttaaaat
   121561 acactttatt taaatattta gtttaaagat aagattttat gtgagtatgt aaaagttaaa
   121621 gatagattta agtaggttat agggattagt aaacaataaa atgaaccatg atgaattact
   121681 ataaattgta tttaaactac attttctact tatagtaata tcaatggttc attttattgt
   121741 tattaagata atacagtatt tataaaaata cttgatataa atatagtaaa ttgtttaaat
   121801 agaattattg ccttaattta ttcaaataat gagtttttaa aatctaagaa atatccttgt
   121861 ggatgaagac atacaggaca agcttttgga gcttctttac ctatatgtat atggccacaa
   121921 tgtgtacata tccattgtat atcttcattt cttctgaata aagtaccttc ttctaaatca
   121981 gtagcatatc taccaaatct atcaccatga actttttcta tttcagctat attttcaaat
   122041 aacttagcta tagattcaaa tccttcttca cgtgctgttt ttccaaagtt tttatataca
   122101 tcatcccact cttgaagctc attatgttgt gcagctttaa ggtgttgtgc tgtcttgtca
   122161 tataaatcta cagggtatgc tcctgatata cttatatttt caccagcaaa ttcttttaat
   122221 ttagaaaaga attgttttgc atgtgatttt tcttggtcag cagtgtaatt aaataaagac
   122281 tctattatat ataatccttc tttttttgct acagatgcag aaatattgta tctatttcta
   122341 gcttgactct cacctgcaaa agctcttaat aaattttctt tagttttact atcttttaaa
   122401 ttcatattct acctctctat cacaataaat ttttacaata tttgattctt tttatttatt
   122461 attccaatag gagatataat tatgctcaat ttagtcagca tttttaaaat tatcattaat
   122521 tttttttaaa attctaagaa attcttgttt ctcattttct gtaatattat gatatgccgt
   122581 ctctttaact tgtgttgaga tgctattaaa agtatcttct ataagcagtg ccttttttgt
   122641 aagctttata tacgtgatac gtttgtcatt tgtacatttt tgtctttcta aatatcctag
   122701 gtttataagt ttattaacca acactgtaac agtggattta tcttttccta ttttagctgc
   122761 aatttctttc attgtaagta ttccattatt ttcataaagt gcagttaaaa tatttccatg
   122821 agtaggtatt aactcaccta aaccattttt actgagttta ttttcaatga atttaatcat
   122881 actagccttt gttttactta tgaaatgtac aatatattta tcttccataa attattctcc
   122941 aatctttaat ctatttaaat tagtttgaca taaaactatt tataaactat aattagttta
   123001 acataaaact aaatgtattg ggaggaaatt tatgaataaa aaatttaaat cactatatat
   123061 tacagaagaa gaaggaaatt ttagaagaaa tatagttgaa agagaagtgg atacattgcc
   123121 agaaggcgaa gttttaataa aagtaaaata ttcttcttta aattacaagg atgcactttc
   123181 tgcaaatggt aataagggtg ttacgagaaa gtatcctcat acaccaggaa ttgatgcatc
   123241 tggtatagta gaatctagta gttgtgatga ttttaaaatt ggagaagaag ttttagtaac
   123301 aggttatgac ttgggaatga atacttctgg tggtttttca gaatacataa gagttccttc
   123361 tagttgggta gttaaacttc ctaaaaatct ttcactaaaa gaagcaatgg attatggtac
   123421 agcagggttt acagcagctt tatctgtgta taaatttata gattctgtag acaagaatat
   123481 gggagatgta ttagtcactg gtggtactgg aggagttgct gtaattgcaa ctaaaatatt
   123541 gattaaatta ggttattcag ttgtagtttc aacaggaaaa ttagaagaac aaaaagaagt
   123601 attactaaac ttaggtgtta aagatgttat acataggtca gaagtggatg ataactcagg
   123661 aagacctctg cttagaccaa gatgggctgg agtaatagat acagtaggag gaaatactct
   123721 agcaacagca ataaagacaa ctaattattg tggagcagta acaacttgtg gtaatgcagg
   123781 aggagttgat tttacaagtt cagtatatcc atttatctta aagggaatta ctttatatgg
   123841 tatagattct gtggattgcc caatgaactt aagattaaaa atatgggatt tattatcaaa
   123901 tgaatggaaa ttagatgatt tagatgatat ggtaaatgta gtatcattgg aagagttaaa
   123961 tgatagtata gattcaataa tggcaggaaa acatgttgga agaacagtag ttgatttaga
   124021 aaaaatatag gtgaataaat ttataattag gattataggt tttaagcaaa aatgagatac
   124081 ttgaaattta cttatgagta tctcattttt ttatgtaaaa agatttatgt agatgtttgt
   124141 gtaaaaagat tattcatgaa caattaattc aaatacatct ggtcttgaat agtgaccttc
   124201 tgaatcaaaa tctagtctac taagtactat tttttctagg tctaaatcag ctattagcat
   124261 ttcttcttta ttgaaaatag gacctgctac atattttcca aaagggtcaa ctatacaact
   124321 tcctcctgga cacataattt ctggttctgc atctagctcc ttatagtagt taaggtcagt
   124381 tgggtacgta tttttttcaa catattgatt gcatccaatt acaaaacatc ttccttctaa
   124441 agcaatatgt ttcatagttg cttgccactc ttctcttgaa tctgcagtag gggctatgta
   124501 aagttttaca cccttagcat aaagagctgt tctagctaat ggcatataat tttcccaaca
   124561 aatcaacgaa cccatttttc catatggagt atcaactact gtaagagtac ttccatctcc
   124621 ttctccccaa atacatcttt ctgttcctgt aggtttaagt tttctgtgtt taccaattag
   124681 cttaccttct ggagagaaaa ataagtttgt gcaataaagg gtacaattta tatcattacc
   124741 atctctttct gttataccca ttgatagata tactcctgct tcttgtgctg cttttcctaa
   124801 taaatctgtt gtagcactag gaactggtac agaattatca tagtatcttt tccaatcttc
   124861 tctaccttcc attgttctac ttccaacaac aaatccaaat gaaagacctc ttggatatgc
   124921 aggtatgaat gcttcaggga aaactacgat atttgcccct tttctaccag cttctttaat
   124981 taatcctaat gctttttcaa cagttttttc taaatccatt atgactggtg acgcctggac
   125041 aacagcaatt cttacatttt tattttcctt gctcataaaa aacctcctaa aaaatattta
   125101 aattttttgc attgaataga ttacatgaga tgttatttga gaactaagat acttatattg
   125161 tgaataataa tgtaactgta tatttaaaat aatacatcat gatttaaaaa attacaattg
   125221 tacagatata attgagttta gatatttaaa tttaacaact tataatattt tttcaattag
   125281 tataaagcct attaatttgt cttatttaca atgaatagta tttacttctt ggcaaacaat
   125341 aattaaaaat aaaactttta tatgattaaa ctctaaagaa aaagtttata aagaagataa
   125401 tataattaag cagaaaataa tgaaaaaaat ttattaaagt agacaacgta aggagaattt
   125461 tatgaaagca aaaatttatt acaatggaaa tatcatcaca atggaagatt caatatgtgg
   125521 agatgctatt cttataaaag acaaaataat aaaaaagata ggaacaaaag aagaagtatt
   125581 tgctttgaaa aataaagata cagaaattat agatttacaa ggtaagacat tgatgccatc
   125641 ttttatagat tcacatagtc acttgatagc ttttgctact actctaaagc tagttccttt
   125701 agaagatgca accagtttta aagacatagt aaaaaaaata caggatttca aagagtcgaa
   125761 taatataaaa aaaggagact ggataattgg attttcatat gataataatt ttttagaaga
   125821 aaataagcat ccagataaga gtgtactcga tagtgcatct tcagaaaatc caatactaat
   125881 ttcacatgct tctggtcata tgggagttgc aaatacattg gggttaaaac agcttggtgt
   125941 taccaatgaa acacgtgacc cagaaggtgg acatataggt agagtagaag gcagtgaaga
   126001 accaaatgga tatttagaag aaaatgcatt ttttaatgta gcatctaaaa taaaacaacc
   126061 atcatcaaat gagattttta attcaattga aaaagcacag aacatatatc taagctatgg
   126121 tattacaaca gctcaagaag gtttaatgga agaaaatcaa tttaacattt taaaagcaat
   126181 ggcaaatcaa aataaattaa aaatggatgt tgtaggatat gtaaatttga aaaaaagtaa
   126241 gtctgtagca gataataata gggagtttat aaaaaaatat ataaatagat ttaaaatagg
   126301 aggttataag attttcttag atggttctcc acaaggtaag acagcatggt tgagtagacc
   126361 atatgaaaat tcagatgatg gttattgtgg atatcctata tacaaagatg aagaagtaga
   126421 aaagtttata gatatttcac taaaagaaaa gatgcaactc ttaactcatt gtaatggaga
   126481 tgcagcagca gaccagttga tagatgcgtt tgagaaagtt ttaaacttaa aagagcaaag
   126541 tagtgaaaat aatataagac cagttatgat acatgctcaa acagtaagag cagaccagat
   126601 tgatgatatg aaagtaataa atatgatacc ttcatatttt gtagctcaca catattattg
   126661 gggtgatatt catatcaaaa accttggaga agatagagcg tttaagatta gcccacttaa
   126721 aacaactata gaaaagggat taatttacac tcttcatcag gatacaccag taatagcacc
   126781 taatatgctg gaaacagtat ggtgtgctgt caatagaatt acaaaaaaag gtatacaaat
   126841 aggagaaaat gaaaagataa gcccattaga tgctttaaag ggtgttacta taaatgcagc
   126901 atatcaatat tttgaagaag ataaaaaagg ttcaattaaa gaagggaaat tagctaattt
   126961 gataatatta gatgaaaacc cacttacaat cgaccctatg aaaataaaag atattaaagt
   127021 attgcagact ataagagaag gagaagtatt gtattctttg aagtagatga aagttagata
   127081 ttttataatc tataaaaaat agttttatat aataagttag gtagaattaa ataagattac
   127141 agttaaatca acaattatat ttttattaaa ttttaattat tgtattgaag atttataagt
   127201 ataattttat tgattctacc tttttaaata actgtagatt tattaaagtt atctatgtac
   127261 tatattaaac aattaagtag ttattaattg cacttattag tttattaaaa aactatgtat
   127321 attactttgc agtaagactt tggaactaaa tctataatag caaatttaaa tttattaaat
   127381 agattaactt ataacatttc atataaaata tattgtgaat ctgagaaaat tactattttt
   127441 ctttgagtat tttttgtaag cagttctaaa agattcaatt tcatacctat tacttgaatc
   127501 attcatcaaa ataattctaa tttaaaaaat aattctatcc aaataataga tatataggac
   127561 tatgttataa tataaaaaaa tttatataaa agttaataac tatacaagat tgataaagaa
   127621 aaagtcaaaa tagataattg attagcaaaa tagatataat tataatgaag ttaaatctag
   127681 caatattctc ctaaaattaa atttcattta tttaattaaa tataatttga caatgggcaa
   127741 aaaagtgagt aaaatataaa taaaataaaa ataacaggtt cggataaaga aaaaggtttc
   127801 ttaacctcaa gcataaagta tattatctaa gttatttgag attaagagat aaatccaatc
   127861 ttaggtactg aggaggaaaa aatgcttgat ataaaaagaa taagagaaaa tttagattgt
   127921 ataaaaaaag caatggaaag aaggggcgaa aaaaacttta accttgatga agtagttaag
   127981 ttagatgatg aaagaagaaa gactcttcaa gaagttgaag ttatgaaaaa tgaattaaat
   128041 acagcatcta aaaatatacc aaatcttata aaagaaggta aagatgtaga aaatgaaaag
   128101 attaaattaa aagagttatc tgataaaatt aaggtaatag accaaaatct taaagaagta
   128161 gaagataaga tggaatacct attaatgaga ataccaaatg taccacatcc agaagtacca
   128221 caaggtgaaa ctgatgaaga taatgttgaa gtaagaactt ggggaaagac tactaccttt
   128281 gattttgaat ctaaggctca ttgggaaatt ggaacagaat taggtatact tgattttgaa
   128341 acagctagta aaataactgg ttcaagattt actctttaca aaggattagg tgctagatta
   128401 gagagagcat tattaaattt ttacttagac acgaatacaa aagtaaatgg atatacagaa
   128461 gttattccac catttatggc aaatagaaat agttttttag gtacaggaca gttacctaag
   128521 tttgaagaag atatgtttaa aatagaaggt atggactatt ttatgattcc aacatcagaa
   128581 gttcctttaa ctaatataca tgctaatgaa atacttaaat ttgaacaatt accgataaat
   128641 tatacagctt atactccatg ttttaggtca gaagctggtt ctgctggaag agatacaaga
   128701 ggtttagtaa gacagcatca gtttaacaaa gtagaaatgg ttaaaatagt tgcaccagaa
   128761 gaatcttaca atgagttaga aaagcttact aacaatgcgg aaactatgct acaattattg
   128821 aatctgcctt atagagtagt aaagatatgt acaggtgatt taggatttac ggcttctttt
   128881 aaatatgatg tagaagtttg gatgccaagt tacaataggt atgttgagat ttcatcttgt
   128941 tcaaactgcg aagatttcca agctagaaga gcaggaataa gatttaaaag agataaagat
   129001 tctaaagctg aatatgtaca cacattaaat ggctctgggt tagctattgg aagaagtgtt
   129061 gcagctatat tagaaaatta tcaacaagaa gatggttctg tggttgttcc tgaagtttta
   129121 agaccatata tgggagtttc tgttattaaa taagagttaa attatgtatt tttaagtaat
   129181 acaataatat agaggtaata aaaaataatt aat
//
LOCUS       DAMJVI010000003       113285 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.003, whole genome shotgun sequence.
ACCESSION   DAMJVI010000003 DAMJVI010000000
VERSION     DAMJVI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 113285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113285
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.003"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            202..1197
                     /locus_tag="PC545_000223"
     CDS             202..1197
                     /locus_tag="PC545_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2455021.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHDGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSDIDKKVRDILEVVELSGYDKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIKSLHRKLGITTIMVTHDQEEALCLADKMVVMNNGEVIQIGTP
                     KEVYKSPKTPFVADFIGTINFIDDDVNMIAIRPEDIKVEFNRDFKEIDVKVGEILDIE
                     FRGFNYRITVEYCSKQIKLDVVSKVVEQMKLCVGSKINFKIPKERTIQYKSEGCA"
     gene            1215..2903
                     /locus_tag="PC545_000224"
     CDS             1215..2903
                     /locus_tag="PC545_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2455022.1"
                     /translation="MITIKNKRRISDSKIQKCFLVFLVIALVVFILFPLISLLKNAFM
                     DTSGSFVGISNFSKYIENPTLIASFKNSIFVSTISSLISLCLAFIYSYAITRTNIRFK
                     NFFKTMGIFPLFAPAMLYGISLIYLFGNKGIFTIIGIEVPLYGPLGIIICQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFFKITIPGIKYSLVSSFFVSFILAFTDF
                     GAAKIVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTVDKIASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIACSFITLLIFILIFVSVIGSVIKLWPYDLSFTMEHY
                     TFKGISGNGIKTYLSSLKISILTAIFGTIFVFFSAYMIEKVDKFPRLRQISYFLSMIP
                     MALPGLALGISYITFFNSASNPLNFLYGSTTIMVIANIVHFYSVCFITSNSSLKMLDR
                     EYELVAKSINIPFYKVFFYVTLPMSITSILEVAVYYFVNSMVTVSALIFLYIPQTQTA
                     SISILKLEEIGYIGPSAAMAVLVFLTNIIVKLLYELTTKKLKTKTQKWQQKEVA"
     gene            2915..3982
                     /locus_tag="PC545_000225"
     CDS             2915..3982
                     /locus_tag="PC545_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2455023.1"
                     /translation="MKFRKLVSLGILSTLLLSVITGCNNASSGSNLKSNNKIKGELTV
                     YTAIEEDSIDTYLTTFKEKYPDIKLNVLRLSVGDITARLLAEKDNPKADVLWGVAATS
                     LIILDDKEMLEPYAPKGFDKVNPVFKDDKKVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPASSGTGYLTVSELIQIMGEEKAWQYMDKLHKNIGVYT
                     QSGSAPAILAASGEYPIGISFGNRGIKLKGEGYPVEVVFPKEGSGWEIESNALVKKDN
                     IKEASKVFLDWAISKDAMNEYSKNFAVTTIDTGNPVPEGFPKNPLEQMIDNDLKSAAR
                     NREAILNKWIEKYDGKTEKES"
     gene            4174..4704
                     /gene="rnmV"
                     /locus_tag="PC545_000226"
     CDS             4174..4704
                     /gene="rnmV"
                     /locus_tag="PC545_000226"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDF2455024.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQVDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREEFIDALKQI"
     gene            4875..5744
                     /gene="rsmA"
                     /locus_tag="PC545_000227"
     CDS             4875..5744
                     /gene="rsmA"
                     /locus_tag="PC545_000227"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDF2455025.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITRGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLDNTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EDIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREVLKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            5810..7075
                     /locus_tag="PC545_000228"
     CDS             5810..7075
                     /locus_tag="PC545_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455026.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEEYEELEEEDEEIEDNEESDRDEGSEE
                     EYIIEEYDEDEYEDYWEDDEDIDEGELKARTEVEDSGDEEIEEFNNYKEIENYNRVKV
                     QEEYIDIEYDEEEEEEEEVFEVNREEDRFVSSKPTKSKKVQKDQNNIEKIKRANGVSQ
                     GESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYL
                     GYLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGF
                     LRYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            7226..8341
                     /locus_tag="PC545_000229"
     CDS             7226..8341
                     /locus_tag="PC545_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2455027.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVI
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEANGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIIAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            8527..9423
                     /locus_tag="PC545_000230"
     CDS             8527..9423
                     /locus_tag="PC545_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2455028.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDENLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            9493..10290
                     /locus_tag="PC545_000231"
     CDS             9493..10290
                     /locus_tag="PC545_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455029.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITHGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(10345..11697)
                     /locus_tag="PC545_000232"
     CDS             complement(10345..11697)
                     /locus_tag="PC545_000232"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDF2455030.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYEGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            11916..12794
                     /gene="purR"
                     /locus_tag="PC545_000233"
     CDS             11916..12794
                     /gene="purR"
                     /locus_tag="PC545_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDF2455031.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDLIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
     gene            12950..13228
                     /gene="spoVG"
                     /locus_tag="PC545_000234"
     CDS             12950..13228
                     /gene="spoVG"
                     /locus_tag="PC545_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HDF2455032.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            13441..14820
                     /gene="glmU"
                     /locus_tag="PC545_000235"
     CDS             13441..14820
                     /gene="glmU"
                     /locus_tag="PC545_000235"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDF2455033.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVMIGNDTIIYPG
                     VMLQGKTRIGSDCIIGMNSSITNSEIGDGTEIKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHDVPDGALAIARERQVIKEGWVEK
                     KNQKDDQSK"
     gene            14915..15865
                     /locus_tag="PC545_000236"
     CDS             14915..15865
                     /locus_tag="PC545_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDF2455034.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
     regulatory      16709..16794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            16956..17477
                     /locus_tag="PC545_000237"
     CDS             16956..17477
                     /locus_tag="PC545_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2455035.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSVKSAALSYYSDTNKIPVTTTDKAGLTILETYMESLPDKADIGGKYKLIKVG
                     NKLVLQIGTDTEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGDELTDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            17629..19305
                     /locus_tag="PC545_000238"
     CDS             17629..19305
                     /locus_tag="PC545_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2455036.1"
                     /translation="MAKKVRIGDKLVEKGYITEEQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            19317..20525
                     /locus_tag="PC545_000239"
     CDS             19317..20525
                     /locus_tag="PC545_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2455037.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTTFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTSVGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILASAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISRQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            20540..22252
                     /locus_tag="PC545_000240"
     CDS             20540..22252
                     /locus_tag="PC545_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HDF2455038.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHDKDKFYVKNFNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIMHEFDLVLDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQIKAKQMKNILK
                     IAPIVILILAIPYLCLIFSNNLANKELENVNGEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGTNNKADNSKSNKTTTDK
                     SSNNETKSSTKSDSNGDKTDNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSNYTFSMTLYLKEGV
                     LTNE"
     gene            22245..23153
                     /gene="pilO"
                     /locus_tag="PC545_000241"
     CDS             22245..23153
                     /gene="pilO"
                     /locus_tag="PC545_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HDF2455039.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGILIKVIILLAILSYGYFGLYPQ
                     YQKYIATKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVKEYEHFYGISTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEIIKNDKLLDRSTNYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            23180..23746
                     /locus_tag="PC545_000242"
     CDS             23180..23746
                     /locus_tag="PC545_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2455040.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGNNYEYNIEMPDNVVKYEVSIETKKN
                     TEVYSVTRIQNNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTGKSIYTVSIY
                     YNESVQESHKNSNLNNKTYTFDVMSRIG"
     gene            23783..24310
                     /locus_tag="PC545_000243"
     CDS             23783..24310
                     /locus_tag="PC545_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2455041.1"
                     /translation="MSKKNSERGFSLIEVLVAMAIMGIVLFAFFNIINTNNKANTKND
                     TDITSLNYVQSEIENLREKIKSGEFDFDSLDKLEDGTVVYEKLIDKSKKVVYDKVLSE
                     GDVSLYDTPYEKITTIKDEDGNLIDKGNITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            24331..25869
                     /locus_tag="PC545_000244"
     CDS             24331..25869
                     /locus_tag="PC545_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455042.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLIFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQEKSISRSKLRNISDE
                     DFNNQDEENIEAERLGYMNNINFYLNKASSDLEKASMELKKLYDLDMLDYRDIEYVDA
                     NIISHRNSILEICKNYTSGDISKINEYILKEDIDSTTLIEAKLVNNDILLKKFLEENK
                     IENEHLNSAFSHTYKALDNISLAMQNMIEYRHTFHIDEPKVEVSNGIPDSQQYYELIQ
                     NPIINSMEYSWNSKWDTLENLLEILPNQTQGFNSLRVHLRNNVRKFEKLSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEIDKTEGITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKNGIKE
                     VEVTDGKKNIIGLGVEENSNSKYKVDAIVNFNLNIDTNVVGNYDIKDKILINHDISSY
                     KKVN"
     gene            25969..27051
                     /locus_tag="PC545_000245"
     CDS             25969..27051
                     /locus_tag="PC545_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDF2455043.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTS
                     EVTMQMAKDIAGESMFKVIEEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPESIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKVGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            27032..27814
                     /locus_tag="PC545_000246"
     CDS             27032..27814
                     /locus_tag="PC545_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2455044.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLMSGRKCRYCYEKISSRYAIVELITGLLFLTVFMLYGISTSTI
                     YYLVLVSLLIIITFIDIDHFIIPDKILVFGAIFSMIFNLLFKEIPVKDSILGVFICGG
                     SVWIIVLLIEFVIKKECMGGGDIKLFAMLGFYMGVKNGLLTALLSVYVGAVYGICVII
                     ISRIKGKEYNSVIPYGPFISIGALITILSGKQLLELYFSMVI"
     gene            27916..28566
                     /locus_tag="PC545_000247"
     CDS             27916..28566
                     /locus_tag="PC545_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDF2455045.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLAFFNI
                     VQFDYINHIFGLLIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDVDIRKQIPFTPFISLSTILIIFTEYNIFNFYYNLLYKFI"
     gene            28580..29140
                     /locus_tag="PC545_000248"
     CDS             28580..29140
                     /locus_tag="PC545_000248"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDF2455046.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEAGQDLADFVLSRFRKEESDNINE
                     ALEKAADAIDSIIRENIDMSMNKYNG"
     gene            29157..32543
                     /gene="mfd"
                     /locus_tag="PC545_000249"
     CDS             29157..32543
                     /gene="mfd"
                     /locus_tag="PC545_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDF2455047.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYMEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDSLDLEKLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTSNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            32574..33569
                     /locus_tag="PC545_000250"
     CDS             32574..33569
                     /locus_tag="PC545_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2455048.1"
                     /translation="MKKVITLVIAMILVVSVTACSSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFAKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSDKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDTSASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            33693..34232
                     /gene="spoVT"
                     /locus_tag="PC545_000251"
     CDS             33693..34232
                     /gene="spoVT"
                     /locus_tag="PC545_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HDF2455049.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            34352..35965
                     /locus_tag="PC545_000252"
     CDS             34352..35965
                     /locus_tag="PC545_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2455050.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNVMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLVFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICVGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            36072..37520
                     /gene="mazG"
                     /locus_tag="PC545_000253"
     CDS             36072..37520
                     /gene="mazG"
                     /locus_tag="PC545_000253"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDF2455051.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLINSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEILPSMSFVDAMFNYLAIDPSEGFKLVDAFEIENSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEHYDYNQEVCIVNGAGVKDLESKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     DFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            37609..37887
                     /locus_tag="PC545_000254"
     CDS             37609..37887
                     /locus_tag="PC545_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDF2455052.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            37959..38201
                     /locus_tag="PC545_000255"
     CDS             37959..38201
                     /locus_tag="PC545_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDF2455053.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYKILE"
     gene            38263..38517
                     /locus_tag="PC545_000256"
     CDS             38263..38517
                     /locus_tag="PC545_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HDF2455054.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            38518..39051
                     /locus_tag="PC545_000257"
     CDS             38518..39051
                     /locus_tag="PC545_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455055.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKLKN
                     KKKSKPKKKKNKTKKRV"
     gene            39057..39341
                     /locus_tag="PC545_000258"
     CDS             39057..39341
                     /locus_tag="PC545_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDF2455056.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINSLKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            39454..40374
                     /locus_tag="PC545_000259"
     CDS             39454..40374
                     /locus_tag="PC545_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HDF2455057.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSDKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQGDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEEFEAMSSFIYDEIDKTLNHIKDRGLKTLIGIGGTITSLSAMNQ
                     ELEIYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            40464..42836
                     /gene="spoIIE"
                     /locus_tag="PC545_000260"
     CDS             40464..42836
                     /gene="spoIIE"
                     /locus_tag="PC545_000260"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HDF2455058.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISMGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAVYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            43187..45079
                     /gene="pepF"
                     /locus_tag="PC545_000261"
     CDS             43187..45079
                     /gene="pepF"
                     /locus_tag="PC545_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2455059.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNNDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDDQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMNDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLEADNVDEKVYNNLIRTVNSNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKGGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            45193..46158
                     /gene="galU"
                     /locus_tag="PC545_000262"
     CDS             45193..46158
                     /gene="galU"
                     /locus_tag="PC545_000262"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2455060.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEVVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEARELEIQK"
     gene            46658..48262
                     /gene="rho"
                     /locus_tag="PC545_000263"
     CDS             46658..48262
                     /gene="rho"
                     /locus_tag="PC545_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDF2455061.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKVNEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQEHRNQRNDYNKTTSVNDSNKSSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            48415..48615
                     /gene="rpmE"
                     /locus_tag="PC545_000264"
     CDS             48415..48615
                     /gene="rpmE"
                     /locus_tag="PC545_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDF2455062.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            48760..49653
                     /locus_tag="PC545_000265"
     CDS             48760..49653
                     /locus_tag="PC545_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HDF2455063.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            49671..50519
                     /gene="prmC"
                     /locus_tag="PC545_000266"
     CDS             49671..50519
                     /gene="prmC"
                     /locus_tag="PC545_000266"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDF2455064.1"
                     /translation="MTIKDIIIKYSDKLKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTKFMGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISETALEIAKKNAIINEV
                     GEKIKYINSDLFTAISDSNIKFDIIVSNPPYIKKQDIETLHKQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKCGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            50563..51627
                     /gene="prfA"
                     /locus_tag="PC545_000267"
     CDS             50563..51627
                     /gene="prfA"
                     /locus_tag="PC545_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDF2455065.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            51756..52436
                     /locus_tag="PC545_000268"
     CDS             51756..52436
                     /locus_tag="PC545_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDF2455066.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGLSGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDVSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            52465..53505
                     /locus_tag="PC545_000269"
     CDS             52465..53505
                     /locus_tag="PC545_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDF2455067.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNVIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVATKINDMIKSNRDKGLKTGVMC
                     ISRNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKGVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            53522..53971
                     /locus_tag="PC545_000270"
     CDS             53522..53971
                     /locus_tag="PC545_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HDF2455068.1"
                     /translation="MNILIVCTGNTCRSPMAEAILRKAIKESGRSIEEYSISSAGIST
                     ANGMGASENSIEVLKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILVQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDIYRSTMREIMYSVNEIVKKI"
     gene            53988..54440
                     /gene="rpiB"
                     /locus_tag="PC545_000271"
     CDS             53988..54440
                     /gene="rpiB"
                     /locus_tag="PC545_000271"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2455069.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLESKGIECVDYGTNNATDSVDYP
                     VYGEIVANSVINKEVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNDA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            54476..55105
                     /gene="upp"
                     /locus_tag="PC545_000272"
     CDS             54476..55105
                     /gene="upp"
                     /locus_tag="PC545_000272"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDF2455070.1"
                     /translation="MSKVVETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            55406..56221
                     /locus_tag="PC545_000273"
     CDS             55406..56221
                     /locus_tag="PC545_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDF2455071.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEGVECIFCNTEKEIEEVLSQHKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRIITNGRAEKNTGVPG
                     AIKGFSKERVMHSQYDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGAKVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            56447..56884
                     /locus_tag="PC545_000274"
     CDS             56447..56884
                     /locus_tag="PC545_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HDF2455072.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            57069..57296
                     /locus_tag="PC545_000275"
     CDS             57069..57296
                     /locus_tag="PC545_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HDF2455073.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            57301..57687
                     /locus_tag="PC545_000276"
     CDS             57301..57687
                     /locus_tag="PC545_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDF2455074.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAVRLFIMAVVLIASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            57690..58394
                     /gene="atpB"
                     /locus_tag="PC545_000277"
     CDS             57690..58394
                     /gene="atpB"
                     /locus_tag="PC545_000277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDF2455075.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGEILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            58463..58723
                     /gene="atpE"
                     /locus_tag="PC545_000278"
     CDS             58463..58723
                     /gene="atpE"
                     /locus_tag="PC545_000278"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDF2455076.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            58846..59343
                     /gene="atpF"
                     /locus_tag="PC545_000279"
     CDS             58846..59343
                     /gene="atpF"
                     /locus_tag="PC545_000279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDF2455077.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKSDLVEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQERQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            59340..59885
                     /locus_tag="PC545_000280"
     CDS             59340..59885
                     /locus_tag="PC545_000280"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDF2455078.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIDGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            59902..61404
                     /locus_tag="PC545_000281"
     CDS             59902..61404
                     /locus_tag="PC545_000281"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDF2455079.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            61432..62310
                     /gene="atpG"
                     /locus_tag="PC545_000282"
     CDS             61432..62310
                     /gene="atpG"
                     /locus_tag="PC545_000282"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HDF2455080.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIAKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMAVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            62323..63717
                     /gene="atpD"
                     /locus_tag="PC545_000283"
     CDS             62323..63717
                     /gene="atpD"
                     /locus_tag="PC545_000283"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDF2455081.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVRKAKGSM"
     gene            63720..63980
                     /gene="atpC"
                     /locus_tag="PC545_000284"
     CDS             63720..63980
                     /gene="atpC"
                     /locus_tag="PC545_000284"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HDF2455082.1"
                     /translation="MASEFSLKVVTPSNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFINVTQDKTVILTESAEWI"
     gene            64442..64822
                     /locus_tag="PC545_000285"
     CDS             64442..64822
                     /locus_tag="PC545_000285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDF2455083.1"
                     /translation="MNIFDIGVDIIEIDRIRKAVDKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRDEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            64837..65298
                     /locus_tag="PC545_000286"
     CDS             64837..65298
                     /locus_tag="PC545_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2455084.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            65319..65909
                     /locus_tag="PC545_000287"
     CDS             65319..65909
                     /locus_tag="PC545_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455085.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDVVELPNTGKNNQYLFVGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            65924..67081
                     /gene="alr"
                     /locus_tag="PC545_000288"
     CDS             65924..67081
                     /gene="alr"
                     /locus_tag="PC545_000288"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2455086.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKICGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIATVPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            67114..67401
                     /locus_tag="PC545_000289"
     CDS             67114..67401
                     /locus_tag="PC545_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HDF2455087.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            67370..67753
                     /locus_tag="PC545_000290"
     CDS             67370..67753
                     /locus_tag="PC545_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDF2455088.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            67924..68751
                     /locus_tag="PC545_000291"
     CDS             67924..68751
                     /locus_tag="PC545_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2455089.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            68744..69685
                     /locus_tag="PC545_000292"
     CDS             68744..69685
                     /locus_tag="PC545_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2455090.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKSF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNAIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIIKTVKEAIIAKRM"
     gene            70108..71202
                     /locus_tag="PC545_000293"
     CDS             70108..71202
                     /locus_tag="PC545_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455091.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDKLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPVIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDKHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            71428..71844
                     /locus_tag="PC545_000294"
     CDS             71428..71844
                     /locus_tag="PC545_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455092.1"
                     /translation="MNVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(72026..72595)
                     /locus_tag="PC545_000295"
     CDS             complement(72026..72595)
                     /locus_tag="PC545_000295"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDF2455093.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLNFNNEVQTDNLIKKLLSLKKIVSSPITIKENHTLIPTQITDLKEGLRIGAYGIR
                     EPNEKSPTIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFDSIPKETHDAQLDYIVTETRIITPNS"
     gene            72749..73906
                     /locus_tag="PC545_000296"
     CDS             72749..73906
                     /locus_tag="PC545_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HDF2455094.1"
                     /translation="MYLVISKKKAIFLGVILVIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGAAKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIIEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTAAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            74510..74992
                     /locus_tag="PC545_000297"
     CDS             74510..74992
                     /locus_tag="PC545_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2455095.1"
                     /translation="MYNNIKNILKENHITFEEYDHEPILSFEKAKEIREKLQYTGVES
                     KSLFIKDRSGNYYIFVTTEGKKLDPKTIKNVLGGKVSICSKEELTQKTQCLPGCVSPF
                     GYEKNISLVIDQDIFNHDKIIFSPGIPEKTFIITASGFKKVINNINNTTYKYIYINEF
                     "
     gene            complement(75691..76845)
                     /gene="nagA"
                     /locus_tag="PC545_000298"
     CDS             complement(75691..76845)
                     /gene="nagA"
                     /locus_tag="PC545_000298"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2455096.1"
                     /translation="MKSFIYADKFFMKHGIKEKGYLSIINGKFGEFQQERPNVTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNRVEGAKIQGIYLEGPFFTEKYKGAQNEKYFKDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTRYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAVKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLNSGSLAGSVLTMDKAVRNIVDWNIATLEDAIKM
                     ATYIPALSCNIDNVCGSIHQGMEADFIVTDKDFNIYETYISGICKYKVNK"
     gene            complement(76928..77653)
                     /locus_tag="PC545_000299"
     CDS             complement(76928..77653)
                     /locus_tag="PC545_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455097.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPGTK
                     VLSFEVVESTKYFSEKLGITPGEKMIKIMRLRLADDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEIIKVADEEFSAGIISDKEAKLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            77920..78849
                     /gene="pfkB"
                     /locus_tag="PC545_000300"
     CDS             77920..78849
                     /gene="pfkB"
                     /locus_tag="PC545_000300"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2455098.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVSK
                     VCKILKENPLAMGFLGGFNGEFIKNELRKLDIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESREEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNEDNVYYVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLGEIDKYQDLVEVKKLS"
     gene            complement(78978..79895)
                     /locus_tag="PC545_000301"
     CDS             complement(78978..79895)
                     /locus_tag="PC545_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2455099.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     VCSDYRIMQFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVSQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVKFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKNFSINTSMHRDSG
                     TKLEQVEILSKGKIQRVKNLNVLEIEEGGNLTLKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECIKAQRLLEKIINSVK"
     gene            80365..81534
                     /locus_tag="PC545_000302"
     CDS             80365..81534
                     /locus_tag="PC545_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2455100.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEKL
                     SRFIDTISNNHGQFRVIFTGAGTSAYIGNSILPYLKNKNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDIPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPEGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIVEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKISTVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            81583..82434
                     /gene="gatY"
                     /locus_tag="PC545_000303"
     CDS             81583..82434
                     /gene="gatY"
                     /locus_tag="PC545_000303"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDF2455101.1"
                     /translation="MALVTTTSMLKKAQDEGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLDEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNRV"
     gene            82760..84661
                     /locus_tag="PC545_000304"
     CDS             82760..84661
                     /locus_tag="PC545_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2455102.1"
                     /translation="MRERIILETLKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFRGNRNITEYRNKFIDNLKLANIRSTIKNVLNEHDLEANLTELEEILAQIIILYIRV
                     SMRVAGNASDLKLYTEDLIIDKLLDKIFRDKKYNLNYDEKYYLKELIKLKTKDKKATI
                     KNIDKNKLQDIMFEFFREIDKKYNSNFLEDKEFFNLFYLHIACLIERIKKNHKIINPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIRNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESVRFADHIGNISSPKQYILGLFDKNHFRCIKDKAKYK
                     DILDSMSKCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDIIYENRVPKIIFMISLIKGNLELHKQISKYLSKIMANKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            84664..85707
                     /gene="ypdE"
                     /locus_tag="PC545_000305"
     CDS             84664..85707
                     /gene="ypdE"
                     /locus_tag="PC545_000305"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2455103.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELKAYSDKIICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLITIGGVKPLAQFVQKVRITT
                     KEGKKIPAVINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTSFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELRKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMEIADKNNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEQYKHIRNFI"
     gene            85722..86027
                     /locus_tag="PC545_000306"
     CDS             85722..86027
                     /locus_tag="PC545_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2455104.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            86124..86459
                     /locus_tag="PC545_000307"
     CDS             86124..86459
                     /locus_tag="PC545_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455105.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            86491..87813
                     /locus_tag="PC545_000308"
     CDS             86491..87813
                     /locus_tag="PC545_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2455106.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGENNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKVFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPVFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLAVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            87823..88893
                     /locus_tag="PC545_000309"
     CDS             87823..88893
                     /locus_tag="PC545_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2455107.1"
                     /translation="MNNTMERLVSLIYKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQILDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAMSI
                     QEYLSLKNEGFEINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVARKIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            88898..90085
                     /locus_tag="PC545_000310"
     CDS             88898..90085
                     /locus_tag="PC545_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2455108.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKESILKRASVGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVVNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDVKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETNIPKAKVMKSDSSFLAWIDVR
                     EVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(90282..91154)
                     /locus_tag="PC545_000311"
     CDS             complement(90282..91154)
                     /locus_tag="PC545_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDF2455109.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWDGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVTYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPFYEQNDFSKHYL
                     EFKSKNDVEKINLNGSFTDDIIPIGKLKNLQLNPEVFKNDALIYTNIDEVSICNNDGS
                     KYVTISMEDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADSINSNKIYKDKKFINKLS
                     KGKVFTASYNINIH"
     gene            complement(91548..91739)
                     /locus_tag="PC545_000312"
     CDS             complement(91548..91739)
                     /locus_tag="PC545_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455110.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            91959..93005
                     /gene="cobT"
                     /locus_tag="PC545_000313"
     CDS             91959..93005
                     /gene="cobT"
                     /locus_tag="PC545_000313"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDF2455111.1"
                     /translation="MSLLESISKNIYSLDNSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFDIIEASNYT
                     YKNMATFDEIDMGR"
     gene            93037..93597
                     /gene="cobU"
                     /locus_tag="PC545_000314"
     CDS             93037..93597
                     /gene="cobU"
                     /locus_tag="PC545_000314"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDF2455112.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCFDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFEHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIVG
                     RINQYIASKSNEVYFVVSGIAMKIKE"
     gene            93607..94380
                     /gene="cobS"
                     /locus_tag="PC545_000315"
     CDS             93607..94380
                     /gene="cobS"
                     /locus_tag="PC545_000315"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDF2455113.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLIGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRDKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKVGFVYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSIIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSELVYLIYIYLLIFMFF"
     gene            94400..95014
                     /locus_tag="PC545_000316"
     CDS             94400..95014
                     /locus_tag="PC545_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2455114.1"
                     /translation="MIRLILIRHALTNDNKKGRLSGHINSCISEEGKLQINKITRYLS
                     NENIDKVYTTPSTRTKDTVEKISKLKLLEIEEKEALREISFGDFEGRTFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLDTKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLEIDGGFAVIDKMNFTDFI"
     regulatory      95081..95260
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            95390..96889
                     /locus_tag="PC545_000317"
     CDS             95390..96889
                     /locus_tag="PC545_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HDF2455115.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKVKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            96928..98304
                     /locus_tag="PC545_000318"
     CDS             96928..98304
                     /locus_tag="PC545_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HDF2455116.1"
                     /translation="MKKILIAGTNSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYKELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDVSVPNADYIYIGGGFPEIFGKELEANESMRTSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLKKGNE"
     gene            98305..99276
                     /locus_tag="PC545_000319"
     CDS             98305..99276
                     /locus_tag="PC545_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2455117.1"
                     /translation="MSILSIYIGYVTDLIVGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLNYNYRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            99308..100378
                     /locus_tag="PC545_000320"
     CDS             99308..100378
                     /locus_tag="PC545_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2455118.1"
                     /translation="MKDLGHGANVDEMARLYGKNPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRENISKYIDINPDFIIPGNGATEVIYLLMKSIKKKLAIINP
                     TFSEYRRSAELNNLDIIDLELDLENNFKLNIDIIKENIKRFDSLFICNPNNPSGNVQD
                     LKELVHLLDKHNKVLIIDETFMEFVEDESKYSLVKYIESNKNIFIIKAVTKFFGMPGL
                     RLGYGLTSNTEIMNKIYEHKEPWTINSFADILSNFIFEDKEYIKNSKEYYIEEREYML
                     QELRNIRNIKVYDTDANFILIKIYKKTTKELKKDLFKQGNILVRDASNFIGLDDSFIR
                     VAIKSHEDNKILIENMKNLLGD"
     gene            100380..101267
                     /locus_tag="PC545_000321"
     CDS             100380..101267
                     /locus_tag="PC545_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HDF2455119.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKYTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVVILEPN
                     KVLDTMKIRLRQDYNKLKVENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     EKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKKMIDALCESNINSVYNK
                     IYTQNIINGGIKGEIEWNT"
     gene            101255..101887
                     /locus_tag="PC545_000322"
     CDS             101255..101887
                     /locus_tag="PC545_000322"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HDF2455120.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVDSIIDALKNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKEKGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAQESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            101896..103122
                     /locus_tag="PC545_000323"
     CDS             101896..103122
                     /locus_tag="PC545_000323"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HDF2455121.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLLLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVAKNDKNKGY
                     LTLKDIDSLSTNSECKSELYKFIRVYGGTGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRKLIGDNFESILGNDKVLKITIFAPQGETVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDDGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRVSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKVSAGIFNTHSKVADARSEILVANLALMGARYEFLN
                     KINQCVTTEEAVELINNSEYREVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            103119..103727
                     /locus_tag="PC545_000324"
     CDS             103119..103727
                     /locus_tag="PC545_000324"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HDF2455122.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYLSKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREIIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            103717..104292
                     /locus_tag="PC545_000325"
     CDS             103717..104292
                     /locus_tag="PC545_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HDF2455123.1"
                     /translation="MRNSEFITGKVPITKEEVRAISISKLNLVNAKNLIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNKKKFNLKNIEVIKGYAPINLGLNVKVESI
                     FLGGTGNNLEEIIEWSKKNLVTGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            104285..105037
                     /locus_tag="PC545_000326"
     CDS             104285..105037
                     /locus_tag="PC545_000326"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HDF2455124.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSNADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSKLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            105021..106142
                     /gene="cbiG"
                     /locus_tag="PC545_000327"
     CDS             105021..106142
                     /gene="cbiG"
                     /locus_tag="PC545_000327"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HDF2455125.1"
                     /translation="MNIEGNIAIICITENGKKLALKINSLVNNCVIYHVKNKKSNLVV
                     EETCVHTVQKKLSDFVGEIFSKFDYIVFIMATGIVVRSIAPYIISKFSDPAILVMDEK
                     GKNVISLLSGHIGGANSLTQYISSLIDANPVITTATDINQKASLDMIAKKLNAHIDNF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLIESKDELRDSFSNTYIEKLVVVSN
                     KINCIEDIENLNLDNKFEHCYSTKKIIKVIPRDIVIGIGCRRNTDSELMYNSLEEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKILKVPFKTFSVDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            106139..106864
                     /gene="cobJ"
                     /locus_tag="PC545_000328"
     CDS             106139..106864
                     /gene="cobJ"
                     /locus_tag="PC545_000328"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HDF2455126.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVEEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKGGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYINGELMITPRGYTV"
     gene            106861..107613
                     /gene="cobK"
                     /locus_tag="PC545_000329"
     CDS             106861..107613
                     /gene="cobK"
                     /locus_tag="PC545_000329"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HDF2455127.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAE
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKTAITCAKLLNIDYIRFERKSL
                     IEEIDYDNKFIVDTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIVAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            107632..109110
                     /locus_tag="PC545_000330"
     CDS             107632..109110
                     /locus_tag="PC545_000330"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HDF2455128.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSHDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESNEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGINAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEENGFNVRVHLKGLGENSYIQDKFVRHAVKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            109140..110045
                     /gene="hemC"
                     /locus_tag="PC545_000331"
     CDS             109140..110045
                     /gene="hemC"
                     /locus_tag="PC545_000331"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDF2455129.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLDGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPHGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSAIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            110035..111534
                     /gene="cobA"
                     /locus_tag="PC545_000332"
     CDS             110035..111534
                     /gene="cobA"
                     /locus_tag="PC545_000332"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDF2455130.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSSYLR
                     EAKPDCELIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLEKISENLMKEGKDKDTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVGLREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNINLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELAPILNDDDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEEKKGEILDILNSNDIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTETINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            111551..112516
                     /gene="hemB"
                     /locus_tag="PC545_000333"
     CDS             111551..112516
                     /gene="hemB"
                     /locus_tag="PC545_000333"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDF2455131.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEDEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINSDINVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGADMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKK"
     gene            112669..113247
                     /locus_tag="PC545_000334"
     CDS             112669..113247
                     /locus_tag="PC545_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HDF2455132.1"
                     /translation="MLYPINLKLDELDVVIIGGGEVAYRKCKNFLEFNKNVTIVSKQI
                     LNKFYDLKGNIKIIKDDYKEIYIKDASVIIAATNNRELNMEIGLYCKAKNKLVNVVDN
                     IEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEERYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLVTLDLEELKKFDI"
BASE COUNT        45361 a        12026 c        20610 g        35288 t
ORIGIN      
        1 atatataaag taaataaatt attgagcgat tattaataag atatgtataa attttaattt
       61 gggaacttgt gttatatatc atgaaaaaag agaaaaaata aaaggtaaat tgtttcttga
      121 taacttgaaa ttggaagtga gaatatttaa ctagagaaga attatttaag tttaagtcag
      181 ctttaggagg aggaattata aatgtctttt ttacaagtaa aaaatgtagg taaaagttat
      241 ggacaagtta aggtgctaaa agacatatct attgatattg aaaaagggga gtttatttgc
      301 ttattaggtc caagtggttg tggtaaaagt acattgctta gaataattgc aggacttgag
      361 gataaacatg atggaaaaat aataataaat gataaggata tgactaactc accacctgaa
      421 agtagaaact ttggtattgt tttccaatca tatgcactat ttccaaatat gaatgtatat
      481 aaaaatatag cctttggatt agaaaataaa aatataagta aatctgatat agataagaag
      541 gttagagata ttttagaagt tgtagaatta agtggatatg ataaaaaata tccatcacaa
      601 ttatcaggtg gacaacaaca aagggttgca ctagctcgtg cgattgcttt agagccagac
      661 tttttgcttc ttgatgaacc tttatctgca ttagatgcaa aagtaagact taaacttagg
      721 gaacagatta aaagtttaca tagaaaacta ggcatcacaa ctataatggt aacacatgac
      781 caagaggaag ctctttgttt agctgataaa atggttgtta tgaataatgg agaggtaata
      841 cagattggaa ctcctaaaga agtctataaa agtccaaaaa caccttttgt agctgatttt
      901 ataggaacaa taaattttat agatgatgat gtgaatatga tagcaattag accagaagat
      961 ataaaggtag aatttaatag agactttaaa gaaattgatg taaaagtagg agagattttg
     1021 gatatagaat ttagaggatt caactataga attacagttg aatattgttc taagcaaata
     1081 aagttggacg tagtttcaaa ggtagtggaa caaatgaaac tatgtgtagg ttctaaaata
     1141 aacttcaaga tacctaaaga aagaaccata caatataaat cagagggatg tgcttaatag
     1201 ctagaggtgg ataaatgata acaataaaaa ataaaagaag aataagtgat agtaagatac
     1261 aaaaatgttt tttagttttt ttagtgattg cattagtagt atttatatta tttccattaa
     1321 tatcactatt aaaaaatgca tttatggata caagtggaag ttttgtagga atttcaaact
     1381 ttagtaaata tattgaaaat cccactctta tagcatcatt taaaaactca atttttgtat
     1441 ctacaatttc atccttaata tcattatgtt tagcgtttat atattcatat gcaataacaa
     1501 gaacgaatat aaggtttaaa aactttttta aaactatggg catatttccg cttttcgcac
     1561 cagctatgct atatggtatt tcacttatat acctttttgg aaacaaggga atctttacaa
     1621 ttataggcat agaagtacca ctatatggtc cattaggaat aattatttgt caaatactgt
     1681 ttacatttcc acaggccttt ttaatattat ctgtagcact ttcgatggct gattatagac
     1741 tttatgaagc agcagatagc ttaggggcta gtgaatttaa gaagttcttc aaaataacaa
     1801 ttcctggtat aaagtattca ttagttagtt cgttctttgt atcttttata cttgcattta
     1861 cagattttgg agcagcaaag atagttggag gaaactacaa tgtattggct actgacatat
     1921 ataaacaagt ggtaggtcaa tttaacatac caatgggagc tactgtctct atggtaatgt
     1981 taattcctgt actaatagca tttacagtag ataagatagc aagtaaaaag caagggatga
     2041 ctataactgc aaagtctatg ccatataaga ttaaggaaaa taagtttaga gatagagtat
     2101 ttttaatagc atgtagtttt ataactttat taatctttat tttgatattt gtttcagtta
     2161 taggctcagt tataaaatta tggccatatg atttaagctt tacaatggaa cactatacgt
     2221 ttaaaggtat atcaggaaat ggaatcaaaa catatttaag cagtttaaaa atatcaattt
     2281 taacagcaat atttggaact atatttgtat tttttagtgc ttatatgata gaaaaagtgg
     2341 ataagtttcc tagactgaga caaatatctt attttttatc aatgatacca atggcattac
     2401 ctggtttggc tttaggtata tcgtatataa cttttttcaa ttcagcaagt aatccattaa
     2461 attttttata tggaagtaca acaataatgg ttatagccaa tatagttcat ttttattcag
     2521 tttgctttat aacttcaaat tcatctttaa aaatgttgga tagagagtat gaattagttg
     2581 caaaatctat aaatattcca ttttataagg tgttttttta tgtaacactt ccaatgtcca
     2641 taacatcaat acttgaagtg gctgtatatt acttcgtaaa ttcaatggtt actgtttcag
     2701 cattaatatt tttatatata cctcaaactc aaactgcatc aatatcgata ctaaagttag
     2761 aagagatagg gtatataggg ccatcagctg ctatggcagt attagtgttt ttaacaaata
     2821 taatagtaaa acttttgtat gaattgacaa caaagaaatt aaaaactaag acacaaaaat
     2881 ggcaacaaaa agaagttgca tagaggaggt aaaaatgaaa tttaggaaat tagtctcttt
     2941 aggaatatta tcaacacttt tattgagtgt aataactgga tgtaataatg cttcatcagg
     3001 ttcaaattta aagtctaata ataagataaa aggagagtta acagtttata cagcaattga
     3061 agaagatagt atagatacat atttaactac atttaaagag aaatatccag atattaaatt
     3121 gaatgtactt agattatcag taggagatat aacagcaaga ttattagcag aaaaagataa
     3181 tcctaaagca gatgtgttat ggggggttgc agctacaagt ttgataattt tagatgacaa
     3241 agaaatgcta gagccatacg ctccgaaagg ctttgataag gtaaatcctg tatttaaaga
     3301 tgataagaaa gttccttctt gggttggaat agatgcttgg atgactggta taacagtaaa
     3361 tactaaagaa ttaagtgata agaacatacc agttcctagt tcttatgaag atttaataaa
     3421 gccagaatat aaaggtctta tatctatgcc taatccagct tcttctggaa caggatattt
     3481 aacagtatca gaacttatac aaattatggg tgaagaaaaa gcatggcagt atatggataa
     3541 attgcataaa aatattggtg tatacactca gtctggttca gcgccagcta tattagcagc
     3601 ttcaggagaa tatcccattg gtatatcttt tggaaataga ggtataaagt taaaaggtga
     3661 aggttatcca gtagaagtag tatttccaaa agaaggttct ggatgggaga tagaatcaaa
     3721 tgcacttgtg aaaaaagata atataaaaga agcatcaaaa gtatttttag attgggctat
     3781 aagtaaagat gcaatgaatg agtattctaa gaattttgca gttactacaa ttgacacggg
     3841 aaatccggtg ccagaaggat ttccaaagaa tccattagaa cagatgatag ataatgattt
     3901 aaaatcagca gcaagaaata gagaggctat actaaataaa tggatagaaa aatatgatgg
     3961 aaagacagaa aaagagagct aagccttgca ataaaattta gtacaaaaag cgcctagaat
     4021 ataagtttta tatctaggct cttttgatat atttatttaa aatgcatatt tctatataaa
     4081 aataaatagt ttagtacctt gattaaatta aataaaaacc ttaaaataaa gagatgtaaa
     4141 gaaaaactaa aaacgaataa aaggtgaatt tatatgataa aagaaataat agtagtagag
     4201 ggtagagatg acgttacagc agttaagaga gcaatagatg cagaacttat aactacaggt
     4261 ggatttggat ttccaaaagg tgtaatggag cgtataaaag ctgctaatga aagacgtggt
     4321 gttataatat ttacagaccc agattttgca ggtgaaaaaa taagaaaaaa aatagcatca
     4381 gaagttccag gatgtaaaca tgcatttcta ccgagagagg aagctaaaaa ggatggagat
     4441 ataggtattg agaatgctac tcctcaaagt ataatagcag ctctgaataa agttagaact
     4501 gaaaatacag aaaaaagaga tgaatttaag caagttgact taattagaaa tggtcttata
     4561 ggtaatgaag aagcatcact tagaagagat gcacttggaa aaatacttgg aattggatat
     4621 ggaaatgcaa aacaattttt aaatagattg aataattatg gagttgaaag agaagaattt
     4681 atagatgcat taaaacaaat ctaaacgtaa tgtttaatgt atgaaaaata tgtatataaa
     4741 ataaattgtg gaattacggt taatttaaga tgaattattt ataatataca ccttatttta
     4801 aatcaatgta aatacatata aataagtata attaataaaa ttaaatatca aaataagtta
     4861 aaaaggagta aataatggat agactatctt cacataatgc aacaaaggaa gtagtacaaa
     4921 aatataattt taaattttca aagtctttag gtcaaaattt cttgatagat agtaatataa
     4981 ttgataaaat cttatctgga gctagaatca caagaggaga taatatcata gaagtaggac
     5041 ctggtatagg aactcttact cgtgagatgg gtaaaatagc agaaaaagta gtggctatag
     5101 aaatcgatag aaatttaatt cctatattga aagatacctt gtcagattta gataatacag
     5161 aggttgtaaa tcaagatata ctaaaagtag acatacagga attagtaaaa gataaattaa
     5221 atggtgggcc agttaagctt gtagcaaatc ttccatatta tataactact ccaatagtta
     5281 tgaaattttt agaggaagac atacctgtaa cagacatagt agtcatggtt caaaaagaag
     5341 ttgcagatag aatgaatgct attccgggta ctaaagatta tggggcatta tctatagctg
     5401 ttcaatacta ttgtgataca gagattgtgg caaaagctcc aagacatatg tttataccac
     5461 aaccaaatgt agattccaca gttataggtc tacatgtaag agataaaaga aaatatgatg
     5521 ttcataatga agacatattt tttaaaacag taaaagcatc ctttggacaa agaagaaaaa
     5581 ctcttcttaa ttcgttggga ggtcttggtt ttcttaataa agatgaaata agagaagtat
     5641 taaaagaagc taatatagac gaaaaaagaa ggggtgaaac tcttagtata gaagagtttt
     5701 cagttctttc aaatataata aatacaaaag tatcttctaa atagaaggta ctttttttaa
     5761 ttttgttaac atatattgtt tagagaaact gtgttaaggg gggattgcag tgggttctaa
     5821 aagaaagttt aaaagagact atataatact agaagctaaa gacataaact ttagatacaa
     5881 agaacgagta ctgccaaaag cttttgcaaa agttgaagta aatgatgaaa aagcaattat
     5941 tgcattatat gtagaaaatc taaaatatgt taaagatggg tataaagcag tagcaataaa
     6001 agatgaccat ggattaatag acttaggaaa tataatttta aatgaacaag gaagaggtga
     6061 atttgtttta gatttagaag attatgatga tgagataaaa ggtattgcat tattgtatga
     6121 aagaaatata cctttaattg gatttaaagg aaataagctt gaaaattatg aggacatatt
     6181 atttcaagag gtagaggaag aagaatatga agaatatgaa gagttggaag aagaagacga
     6241 agagatagag gataatgagg aaagtgatag agacgaaggt agtgaagaag aatatattat
     6301 agaagaatat gatgaagatg agtatgaaga ctattgggaa gatgatgaag atattgacga
     6361 aggagagtta aaagcaagaa cggaagtaga agattctggt gatgaggaaa tagaagagtt
     6421 caataattat aaggaaatag aaaattataa tagggttaaa gtacaggaag aatatataga
     6481 catagaatat gatgaggaag aagaggaaga agaagaagtt tttgaagtaa atagagaaga
     6541 agataggttt gtttcctcaa aacctactaa atcaaagaaa gttcaaaaag atcagaataa
     6601 tattgaaaaa ataaaaagag ctaatggagt aagtcaagga gagagtggaa ataaacaaac
     6661 cagtacatta cttatgccaa gacaaataaa aaaaggtttg aaattattta aagaagtaaa
     6721 accttttgta acagattata tagataagac aagatggtgg aaaatagaga taaatcctac
     6781 aacattgtgt ggatatacaa tgccttattt aggatattta aacgtgttga attacaccat
     6841 gtatagtgat gcagttttac attcatataa atacagacac tatttatttg gagtccagta
     6901 tgatgaatat aataagagaa aacattatat ttatgctatc ccaggaaata gaaatgaaca
     6961 gccagacaat ggaagcacag gatttttaag atatcaagct tgtgataata gaacaaatag
     7021 tttaggatat tggctatgtt ttatagattc tagacaaaga aatatagtta ggtaaaaata
     7081 ataaaaatga aaaaatttag actcaattct tattttactg aaaaaattaa tgaaatgtag
     7141 tacaaaactt taaaaactat tgataatatt taaatattac actacaatat atcttgaaat
     7201 acctattata ttatggagga aaattatgat aaacgagcaa agaattttaa atgagtttct
     7261 tgaattagta cagatagata gtttatcttt aaaagagggg aatgttgcta aagtattagt
     7321 taaaaaatta gaagaaatag gttgttcagt agtaatagac aatgctggtg aaaaagcaaa
     7381 tggagaaact ggtaatataa tagctacatt aaaaggaaat aaagaaggaa agaaaatatt
     7441 atttagttca catatggata cagtaactcc aggtataggg gtaaaaccta tagtggatga
     7501 agcaaatgga ataataaaaa gtgatggtac tacaatatta ggttctgatg ataaagcagg
     7561 tatagcagct atattagaag gcttaagata tataaatgaa aataatatag aacatactga
     7621 tatacaagtt gtattctcta tatgtgagga atgtggatta gttggagcta aaaatttaga
     7681 ctatggaaaa atagattctg aatatgcatt tatattagat agtggtggtt ctccaggtga
     7741 aataatagta aaagctcctg cacaagatgt aataaatgtt aagatattag gaaagactgc
     7801 tcatgcaggt cttgaacctg aagctggaat aagtgctata atggtagctg ccagagcaat
     7861 agaaaatatg aatttactta gaatagacga agaaacaact gctaacatag gaattataaa
     7921 cggtggaaca gctacaaata tagtaacagg agaagttaat ataattgcag aagccagaag
     7981 tctaaaagaa aataaattag atgttcaaac taaacacatg gtagagactt ttgaaaaagc
     8041 tgcaaaagat tttggagctc aaattgagat agatgtcaat agagcatata caccaataga
     8101 tgttagtgag gattctgaaa taataaaact tgcaaagaag gctttttcaa acttaggtat
     8161 agaaggtcat actgaatcta caggaggagg tagtgataca aatatattaa gtaagaatgg
     8221 tattgaagct ataactttag gaattggtat gaaaaatgct catacactaa gtgaacatat
     8281 agctataaaa gacttatatg attctgctaa aatggtagtg gagataataa aggaagcata
     8341 aatttaaata atagtgtata atatatatat cgcctagctt aagctaggtg atttttcatt
     8401 ttatagaaga ttgataatct ctattgaaag taaagagtat acatttgcat ataagagagt
     8461 gtttaattta tgttcaaatg taggttctta ataatattta gattgtttca taagggagga
     8521 ataaatatgg aaacaaggta tgaagaagca aataaaataa caatacaatc aatactttgg
     8581 aatgtagtat taacaataat taaggttata gctggtgtta ttggtaattc tagcgctatg
     8641 attgcggatg gattacattc agcatcagat attataagtt ctataggagt actaataggc
     8701 aattatgtat cttctagacc tggagataga gagcataatt atggtcatga gaaagcagag
     8761 acattagtat catttgtgtt atcaatattg cttattttcg tttctataac aataggtata
     8821 gaggcaatta aatctttatt taacttagat gcattaagtg ttccatcaat attaccatta
     8881 gtagtatcag ttatttcaat cctgataaaa gagtatcaat atagaataac aataaaagtt
     8941 gctaaaaaga taaattcacc agccctaaaa gctgatgctt ggcatcatag gtcagatgct
     9001 ttatcttcag tagctgcttt tataggtata ggtggttcta ttttagggtt taaaccatta
     9061 gacccaattg cgtcagttgt agtggctata tttgtagcta aagtaggaat tagtatactt
     9121 ataagttctg ttaatgaatt gatggatgtt tcagttgatg aagaagagat aaaagaatta
     9181 aaatttattg tagcagatac agaaggtgtt aaaaatcttg gtgatataaa gactagaaaa
     9241 catggagcta tggcatatgt agatttgact atatgtgtgg atgaaaattt gacagttaag
     9301 caaggtcatg atattgctac caagcttgaa aaacatataa taaaacatat ggaatttgta
     9361 aaaggcataa cagttcacgt tgaaccatgt actaattgcc aaggaaataa gtgtaataat
     9421 taattaaaac ttaaaaaaaa gtgtaaaaag ttatataatt gttttatgat tataaactct
     9481 aggggtggtt aaatgagcaa gaaaagaaaa ctaaagaaaa aagctgcaat attaatagga
     9541 gtagttgttt tctttataat atactttata tcctttagaa taggaataaa tatggaaaag
     9601 gataaaagtg aagtgccaaa gaaggtagaa aaagaagaag aagataatag gtctttaagg
     9661 actcaaataa aagagaagaa gaaggtttat atatcagata aaaatataga aaacataaaa
     9721 attgaagaag atatgtggga tgaaataaag ttatttttct cagattttaa gaaagtaaga
     9781 aatactactt cttcttacga agcagtttat gaaggttatt ctgatgatgg agttaaattt
     9841 tctactgacc ttaacttttt tagagtatat actgtaaata aagaagaata ttataaagta
     9901 ccagttagtt ctaaggatgc ttttaagaaa cttttagatg agagcatata tacttcgttt
     9961 gaccttttaa gtcattataa agattggaaa gaggttacta taactcatgg aaaagaaatt
    10021 aaaaaagttc atagatggaa atttgatgat ttagcacata agatgatttc aaagagggtt
    10081 gtaggaaaag ttcaacctga gaaaagtaag gaaagaagtg actataattt tactataaat
    10141 ataaaaggtg ataattttga tgtaaaagtt gatacaatgg gaaaagatta tgtaaaagtt
    10201 acttctaaga aaaatgtatc ttactatgag gttcatacaa gtttatttga ttaccttaaa
    10261 aatgagatat ttgaaattgg aaagaaataa gactttaaga aggctatcaa attagatatt
    10321 tgatagccta aattatttta tatattattt ttcaaccaac aagtctgcaa ccttataaat
    10381 tggaccagca ccagcagtta ttactaaatc attatcctta acattttcac gtaagtattt
    10441 tactatatct tcaaattctt ttatatagat ggcatctaca ttgttttgat ataatttctc
    10501 aaccaagtcc ttagaatgta tatctcctgg gtctttttcc ctagcagcat atatatctgt
    10561 tatgataact ttatcagcag catagaatgc ctctgaaaat tcacctaaaa gagattttgt
    10621 tctagtataa gtatgtggct ggaagataca ccataaagtt gattttttta attttttggc
    10681 agcagctaaa gttgctttta actcagttgg atgatgtgca taatcatcta ctactaaagc
    10741 attcttataa taccctttag tttcaaatct tcttccgaca cctttataaa tttttatatt
    10801 ttttcttata gtctctaagt caataccaga aacaagagct gccattatgg cagaagaagc
    10861 attgtatatg ttatgaagac catatacaga aagttcaaat tctcctaaat ctcttccttc
    10921 atattctatt ctaaatattc catgaccact attgtcaaag tgaatatctt ttattacagc
    10981 atcattgtca gaatctctgc cgtattttat aatagtagct ttaacatcat acaatatgtc
    11041 atcaatattt tcatcatcgc catttattat gaaatagcct tctgggggta ataattttcc
    11101 aaatttgtta aaagaagctt tgatttcatc tattccagag aaataatcaa gatggtcttc
    11161 ttcaacattt aaaactatag atatctttgg gttaaaatta aggaaactat ctacatattc
    11221 acatgcttca gttataaagt ggttggaatt acctatctta acattaccac ctatcatact
    11281 taagttacca ccaactagta tagttgggtc taagtctgca tattcaaaga ttgtagataa
    11341 catagatgtt gtagaagttt ttccatgtgt tccagaaact gctatagaat ttttatattc
    11401 cctcatgatt tggcctaaaa aagtcgctct gttcattacc aatttatttt tttctttagc
    11461 agccacaagt tcttcattgt ctggatgaac tgctgctgta taaacaacca tatttacatc
    11521 atctgatata ttatcttttt tctgaccaat ataaatagtt gccccttggt ccctaagttt
    11581 gtctaatagg tatgattcgt ttgaatccga acctgaaact tgataacctt tattgataca
    11641 tatttcagct aatgcactca tgctgatacc gccaattcct ataaagtgta tattcataat
    11701 aaaaccacct ttctttttaa ttaagtccta caatatgaac tatttctgtg gtataataat
    11761 ataatgttca atattttata attaatatgc atataatatt atatgtattt tatttttgaa
    11821 tataataaaa cataagtaat attataccac aataagaatt gttatgcatt agttagttta
    11881 acgttatata aaggtacttg taggaggaaa taataatgaa atttaaaaga acagagagaa
    11941 ttggagcaat agttaaaata ctgtctgata atccaaataa aatatatact ttaagttatt
    12001 ttacaaatca atttaatgca gcaaaatcaa caataagtga agatttatta gttgtaaaaa
    12061 atgtatttga gaagctacat ttaggtaaag ttataactat ttcaggagca gcaggaggag
    12121 taaaatatat acctaaaact tctatagcag aaaatgcaga gttcttaatg gaattatgcg
    12181 agagaatatg tgacaagtct agaatactat ctggtggttt tttatacttg attgacctaa
    12241 tttacgaccc tacaatagct gctaagatag gaaagatatt cgcatcaaat attgagtatg
    12301 tagatgcaga ttatgttgtg acaatggaaa caaaaggtat acctatggca cttatgactg
    12361 caaaagctat gaatttacct ctagttataa taagaaaaga tataaaggta tcagaaggtc
    12421 ctacacttag catgacatat gtgagtggaa acagttctaa agttgaaagt atgagtttgc
    12481 caagaaaagc tcttaaacca gatagtaaag ttataattat agatgatttc atgaggggtg
    12541 gaggtactat aaaaggaatg gtagacctta tgaatgaatt tggtgcagag gttataggta
    12601 ctggagtatt catatctaca acgaatcctt cagaaaaaat ggtaaaagat tatatttcgt
    12661 tgattcaatt ggatgttaat ggagataaaa tagtagtaga accaaactta aagactttta
    12721 aagatgaata tagaaatgaa gatttagacg aagaagaaga ttcagaattt gaatttgaga
    12781 tagatgaaga ttaataaaaa aaatatttag aaaaatatgt aaaaataaaa agaggatatc
    12841 cctagttgtt catagaatta tttaaaaaac agtttatatt aatttattcc agcgataata
    12901 attttaatat aaattaattt ctgtcaaaat caaaggggga tattttggta tgaagataac
    12961 tgacgtaaga gtaagaaaat taacagaaga aggtaagatg aaatgtatag tttcaataac
    13021 tttcgataat ttgtttgtag tgcatgatat aaaagttata gaaggccata atgggctatt
    13081 tatagcaatg cctagcagaa aagtaggcga aggaaatttc agagatatag ctcatccaat
    13141 aaatgcagaa atgagacaag ttttagaaga tgcagtttta caagcgtatc atgaagcatt
    13201 agttcaatgg gaagtagctg ctgaataata ataaatatat aaaatggaga gagcccgaac
    13261 atttaggctc tttttaaatg tagaatttct attaaaatat gtacttttta gtaaaaaagt
    13321 gctatattat tagtgataat gtatatgttg tatacaaatt gttagataaa tgtattataa
    13381 tgtatattga ttaagagaat aatacgaata aaattaaatt agattgaatg gagtgtatca
    13441 atgaacttta aagctataat ccttgctgct ggcaaaggaa caagaatgaa gtctaaatac
    13501 ccaaaagtta tacataaagt atgtggaaaa gaaatggtta accatattat agatgtttca
    13561 aaaaaatcag gggtaaaaga tactgtcgtg attttaggac atgaagctga tgtagttaaa
    13621 gaaaagcttg cagaagaaat tataatagcc atgcaaacag aacaacttgg gacaggacat
    13681 gctgtaaaga tggctaaaga atatattaat gatgaagata caatagttgt actttgtgga
    13741 gatactccac ttataaaaga agagacacta aagagactat ttgaatatca tatagaaaat
    13801 aaatatcatg ctacagttct tacaacaaga gttggaaatc caacaggtta tggtagaata
    13861 ataagagata agaagggtga ccttcttaaa atagtggaac aaaaagatgc aaattcagaa
    13921 gaaaagatga taagtgagat aaactctgga atttattgtt ttaatggaaa gagtcttaga
    13981 gaagcactag atttgctaaa taataacaat tcacaaggtg aatattattt aactgatact
    14041 gctaaaataa tgagagataa aggattaaaa gttggtgcat ttgcaggttc aactattgaa
    14101 gaacttatgg gcgttaactc aagagtagaa ttatcaaagg cagaagaaat tatgagacga
    14161 agaataaatg aatctcatat ggtaaatgga gtaactataa tagacactaa ctctacctat
    14221 atagaatctg atgttatgat aggcaatgat actataatat atccaggtgt tatgttacaa
    14281 ggaaaaacaa ggataggttc agattgtata attggaatga attcatctat aacaaattca
    14341 gaaataggtg atggtacaga aataaaaaat tcaactataa ttgatagtaa ggttggagaa
    14401 aattctactg ttgggccata tgcatactta agaccaaaga gtgacttagg aaataatgta
    14461 aaaataggcg attttgtaga agtaaaaaat gctattatag aagatggctc aaaagcatct
    14521 cacctttctt atataggaga tgcacatgtc ggtaaaaatg taaatattgg atgtggagta
    14581 gtatttgtaa actatgatgg taaaaataaa tttaaatcaa tagtaaaaga caatgcgttt
    14641 ataggttcaa attctaattt agttgcacca gtagttgtag aagaaaaagg atatatagca
    14701 acaggttcaa caataacaca tgatgtccca gatggtgctt tggcaatagc aagagaaaga
    14761 caagttatca aagaaggatg ggtagaaaaa aagaatcaaa aagacgacca aagtaagtaa
    14821 ttatgtagtt tattattttg gctaaaaaat ttagctcgaa attattataa attatattca
    14881 ttagtatttt aaaattttca ggagggttac ataaatgaat actagcggaa gcgagattaa
    14941 aattattgca ggtaattcat cgaaagagtt agcacaaaaa atagctgact atataggtgt
    15001 atcagtacta gattgtgaag taggtacatt tagtgatggt gaaatatgtg taaacatgaa
    15061 tgagacagta agaggttgtg atgtgtttgt agtgcaatct actaacagcc cagtaaatga
    15121 taacttaatg gagttattaa tcttaattga tgcgttaaag agagcatcag caggtagaat
    15181 aacagcagtt attccttact atggatatgc aagacaagac agaaaggcta aggcaagaga
    15241 tccaatcact gctaaattag ttgcaaattt aataactgct gctggagcag atagagtatt
    15301 aacaatggat ttacatgcag ctcaaataca aggatatttt gatataccat tagaccactt
    15361 actaggtgga acaatattag caaattattt taatgaaaag aaaatagaag atttagtagt
    15421 agtatctcct gacttaggaa gtgttactag atcaagaaaa tttgctaata ctttaaatgg
    15481 agaagttcca atagctataa tagataaaag aagaccgaaa gctaatgtat gcgaagttat
    15541 gaacttaata ggtgatgtta aaggtaaaaa tgttatacta ttagacgata tgatagatac
    15601 agcaggaaca atagttaatg ctgcaaatgc acttaaagag tttggagcaa aagatgttta
    15661 tgcttgttgt actcatggtg tgttatcagg tccagctatt gaaagaattg ctaattcaga
    15721 aataagtgag ttaatagttc ttgatactat acaacttcct gaagagaaaa gaatagataa
    15781 gataaagata aaaacagtag ctcctttatt tggagatgct ataagaatga tattctctaa
    15841 tgaatcagta agtaaattat tctaatttat tgtatttatg agagttgttt aaaaggatat
    15901 aaaccttttg agcaactctt ttttgatttt ataattatgg aatgttattt ttttcttgtt
    15961 agaaaggtat atattattgt aaaaagagat tatagtttaa aattatgtat atttaagtaa
    16021 aataaactta ataaagggca tattattcaa aatattatga atataaaaaa tattacaaaa
    16081 aagtattgaa aaaattcaga ttatgtagta ttatgtaaat ataaataaag aaagaaagag
    16141 gtgtttccaa tgggaaggtt agaagatgaa cccctaataa ttaaatgatt cagtcacaat
    16201 ctaaagtgaa tataaaaatc tattgaaagt ttatttataa gaattgaaaa tagtatctat
    16261 tctatcttta gtgtttgtga gagggttctg gattatataa gattactgta aagaagtaat
    16321 tttgcacaat aagcatatct gtaaatgaag agtttcgttt atataatgat tgtggctgaa
    16381 tcataactat aatagataga atgtttataa tagatgattt tataaaatac tatattgtaa
    16441 tagcttttag ctttcaaata aatattagca aaggatggaa atgacaatga taaaactaaa
    16501 tgataattta taaatatctc cttacgatat ataataattg tagggagatg tttctttttt
    16561 ttgtagagta taatttttct agaaattctg tttcaatatg taaaaagttg gtatatataa
    16621 atagtataaa cagtgttgct aaaatatggt agatattata taatataaat ggatatgtat
    16681 atacattatg agttatataa tttgaattta ataaaataga aacgttgatt tatgttctgt
    16741 aatgtgggca ccttggagca tattgagtta gtggtgcaac cggctatgaa attgtattta
    16801 tttataaata ctattatttt catagctttt tttatttaaa aaatggttat atgttagaaa
    16861 taaatttggt tagtacgttt aaataatttt acttagattt ttaaccagat atattataag
    16921 taagtattaa ttaaaaaatt taagggggaa taaaaatgaa gttaaaaaag aataaaaaag
    16981 gtttcacttt agtggaatta ttggtagtaa ttgcaattat aggtatatta gcagtagtgg
    17041 cagttccagc tttatttagt aatataaaca aggctaaggt agcaagtgtt gagtctgatt
    17101 atagctcagt taagagtgca gcattatctt attattcaga tactaataag ataccagtta
    17161 caacaactga taaagctggt ttaactattt tagagactta tatggagtct ttgcctgata
    17221 aagctgatat aggtggaaaa tataaattga ttaaagttgg taataaattg gtattacaga
    17281 taggtacaga tactgaagga gttacattaa cagaagcaca atcagcaaaa ttattgagtg
    17341 atataggtaa agataaaata tatacaggtg ttacaggaga taattttgga gacgaattaa
    17401 cagatactac aaaaatagat aataaagctc tatatatagt acttatagat aatactgtga
    17461 tggactcaac aaaataatta aaactatatt tttataaaga atattgtaaa tttaatgaaa
    17521 aatataatat aaaagacagg aagaaaatct tcctgttttt ttatactatt ttctttaatg
    17581 ttcaattatg aatgatgata taattgagat agtaaaggag tgaaacctgt ggctaaaaaa
    17641 gttagaatag gagacaaact ggtcgaaaaa ggatatataa cagaagaaca gcttaaatgg
    17701 gcactatcag aacaaaaaaa cagtggaaaa agactaggag aatttctagt acaagaaggg
    17761 cttatagata gtaacttact aataagtgta ttaaaagaac tcttggatat agaaagtata
    17821 tttttagaag ggacagaaat agacacttta gctacaaaaa tggttccaga aaatatatgc
    17881 aaaagatata ctgtatttcc atttaaaatt gatggaaata aaatatgtct agccatgtca
    17941 gacccacaag atagagaagc agttcaagat gtaaggcgta tgtctggtaa agacgtagag
    18001 atatttatat caagtacaga agatataaac aaagcaatag gtcatgctta tgcacattca
    18061 gaaataaata aggcaatgac tgagtacaac aaaaatagaa ctggtggagt aagagaaaca
    18121 gtaatacttg aagaagatgt aaatgctgct ccaatagtac gattagtaaa taacatacta
    18181 gaaaatgctg ttagaatgga agcaagtgat atacatatag aacaaagtga aaattacatg
    18241 cgtgttaggt ttaggataga tggaatgtta agagaatata tgaggatgaa ctcagcacca
    18301 tataaggcag taatcagccg tatcaagata atgtcagata taaatatatc tgaaaagaga
    18361 ataccacagg atggtagaat ctatttaaaa gtggataata aacccataga ttttagggtt
    18421 tctacaatgc caactaacag ggatgaaaag atagctatga gggttcttga taagagtaat
    18481 tttatggtta gtaaagaagt actaggaata gacgaacatg gctctaagat atatgatgaa
    18541 ttgataaaca ctccttatgg tcttatattg gttgttggcc ctactggaag tgggaaaacc
    18601 actacattgt attcaatgtt aaatcaacta aatacagaaa atagaaatct attaacaata
    18661 gaagacccta tagagtatga attacctggt gtaaatcaat ctcaaataaa tgaaaaggca
    18721 gggcttactt tcgccagcgg acttagagct tttatgcgtc aagacccaga tataataatg
    18781 gttggagaga taagagatac tgagacagca gaaatagcaa ttagagcttc tcttacagga
    18841 catttagttt taagtacatt acatgcaaat acagcagttg gagcaatttc acgtctttta
    18901 gatatggatg tggaaagttt tttaataact tcatctgttt taggtgtaat atctcaaaga
    18961 cttacacgaa aaatatgtga gcattgtaag gtttcatatg aagcagatat tggagaaaaa
    19021 aaggctcttg gtattgatgt aaatgaatca gtaactattt atagaggaaa aggttgtgaa
    19081 aggtgtaata atactggata taaaggacgt ctaggtatat ttgaaatgct tgaaattact
    19141 ccagaaataa aagagttaat agattcatct gcaaatcaaa gagaaatttt aaagatggct
    19201 agaaaacaag gtatggttag cttaaaagag gatatagtta aaaaagtttt aaatggaaag
    19261 acaacagtag aagaaatgat tagaataatt ttgatgactg attaaaggat gatattatga
    19321 aaagttttaa atacacagta ataaaagagg acggaaaaaa gcaaactgat gtgattgaag
    19381 ccaacgattt taatgaagct agaaatcttc ttagaaagag aaatcttaga gttattgaaa
    19441 tcaaagaaag caaaaataaa aatataagat tatctagtag aaagagaaaa aaagacttag
    19501 gagctgacca gataagccac ttttgtagac aatttgctat tatagtatct tctggtataa
    19561 atagtatttc tggattagaa acattagcta gaaggtcaac taacattaca ttaagagaag
    19621 aaattaatcg tatagtagca gaaataaaag taggttctac tattgcagat tcaatgcttt
    19681 catcaaaatc aaagtttcca aagttacttg gtgctatggt agctacaggt gaagctacag
    19741 gtaaattaga tgaagtatta aaaagtatgg cttcatttta tgcaagtgaa cacagggtta
    19801 aacaaaagct tagaaatgct gcaacatatc cattaatagt tttgatttct tcatttataa
    19861 tgatttttat atttacaaca tttatggtgc ctaagatgat taattcaatt acaacagtag
    19921 gagcaactct tccacttata acaagaattg ttatggggtt tggtatgttt atgaagaagt
    19981 tttggataat agtactttta gttattatat tatttatcta tcagtttaaa aaatatctaa
    20041 aaacgtcagt tggaagggct cataaagata gagttataaa taagatacct gttttaggaa
    20101 aaggaataaa ttgtatggta gctacacgat tttcaagagc attgtatcta tttgtatcaa
    20161 caggataccc attagttcaa gggcttgatt atattataga tagtgtaaat aacactatgg
    20221 cagagaagat tttagcatca gctaaagatg gaataacaag aggagaaggc ttagcagaaa
    20281 atttagagag atatacgtat tttgactctg tgttggtaca gatgatagct ataggggagc
    20341 agacagggga attagagaat ataagtagac agatggcaga attttatgag tatgaatccg
    20401 agatttattt aaataggatg gcctcaatga tagagcctgt tttaataatt gcagttggta
    20461 ttatagttgc gatactggtt gtatcaatat tcatgccaat gcttagtata tatgatgcca
    20521 tgtaggaggg aaaggacaaa tgaacaaggt ttatgtatca tattatggcg attctatttc
    20581 catagttgag ggcagatata aacacgataa ggataagttt tatgtaaaaa attttaatgt
    20641 gttttcaata gaggatattg cacaagattt ttctaaggaa aagtatgctc tattgagata
    20701 cgcattagaa aatgttaagt taaaatcaag acatgtagtt ttttgtttaa atacaagaga
    20761 tgtaatttta aaaccccaaa aacttcctaa tatggacaaa aaggatttag acggatttat
    20821 gaagctagag atggacgaaa tgatggcttt ggagtcagat gaatatgtat tttcgtatga
    20881 agtatctagt gaaacacctg ataattcaga tggcgatgaa tcaagcttaa atcttattat
    20941 tggagctata caaaaggaag aggtaaatgc aattgtagag ataatgcatg aatttgattt
    21001 agtattagat agaatagata cattatcaac agcttactta agaatgctta aaaatttaga
    21061 ctacaatgat ataatggtaa ccaatataag tgacaatagc acaattataa atatatataa
    21121 gaaagatact ttatttatta gtgataatat acctataaag ataactgcta aagatatgga
    21181 catgcaatta ttaaatgtag tagaagaatc aaaaggtctt atgaattact attcgtcaag
    21241 aaactttggt aaggttacgg atactatatt aatattagga aaaagatatt ccaataaatt
    21301 aatttatgaa acttttagct ctgcattttc aagctatgtt tctcaaggcc ttactgagat
    21361 aatggatata tctgatatga taactggaga tatagatgaa gatgatataa attcaattgt
    21421 agagctttta ggaagtatga tagaacctaa agggaaaaga gattttgaaa atataaactt
    21481 tttaccaatg gatatactta gaaaacagat aaaagctaaa caaatgaaaa acatactgaa
    21541 aattgctcca atagttattt tgattcttgc tataccttat ttatgtttaa ttttttcaaa
    21601 taatttagca aataaggaac ttgaaaatgt taatggtgaa ataagaaata ttgaatctgc
    21661 atattatcag attggaaata tagaaaaaat gatacagagt aaaagcgaag aaataaaggt
    21721 atatgatatg ttaattggga aaaaggtaaa gtgggaaaat ttattggatg atatagataa
    21781 aaatactcca agtaaagttg aactaacaag tttatcaaca acttatcaaa atcttggaac
    21841 taataataaa gctgataatt caaaaagtaa taagactaca acagataaat caagtaacaa
    21901 tgaaacaaaa tcaagtacga aaagtgatag taatggagat aaaacagata atgatttaaa
    21961 tgaagataag actacaaatg tgtcagaagc attaactgga aatagtgaga ctaatagtaa
    22021 taacacggat aaagatgata aaactccatt gtatgataag ataccaaatg ttataaatat
    22081 agaaggaaat gctaaagatt cctcttatgt aggtcaattt ttatataaat taaagggatt
    22141 atactatttc aaggatgtta aattacatag cataaaaagt aatgcagaag attctaacta
    22201 tacatttagt atgacattat acctgaaaga aggtgtatta actaatgaat aaagatatat
    22261 ttaaaaaaga tataaaagat atatttaaaa tggaagttag tctaaaaggg attcttataa
    22321 aggttataat tttattagct attttatcat atggttattt tggactttac cctcaatatc
    22381 agaaatatat tgctacaaaa aattctgtaa aacaggctac tgaaaaatta aatatatata
    22441 aagagaaaac tgcacttatg ccaaaatatg aaaaacaact tgaggaagtt gaaagtgaat
    22501 taaaagaaaa aagcaaaaat cttttatatg atatgcaaga tggtcttttt ttaataggtt
    22561 tagataaaaa aatgaaggct tataatattg acttaaccag ttatgacgtt ggaaatgtta
    22621 aagaatacga acatttttat ggtatatcaa caacaataag tgttagaggt gattatagaa
    22681 atgtcaaaaa attaatggct tatttagaag aacaaaaaaa tgttactcaa atactagatt
    22741 atacaatgac ttcatatata gaaccagaaa aagaagagga agaaacaaca aacaataagg
    22801 atttaattaa aaatgtgaac ataaataaag atacaaaaat atactgggtg gaaggcgaaa
    22861 ataagtggca tttatataag gattgtccat tgataaaaga gggagagatt ataaaaaatg
    22921 acaaactttt agataggtct actaattatc tatgtaatac ttgtgtactt agagctgcaa
    22981 cagaaaaaga agcaaaaaaa gaagtcgtag ctccaaaggc agaaggtgta gtagatgcta
    23041 catttaaatt tataatgtac actaaagaga atactacaat gatacttgat acagataatc
    23101 catcttcatg gagaccagga aaatataatc catttaagtc tagtgcgaaa taaagctaag
    23161 taagctttaa agataggtga tgagtttgta caaaaataat gaaaaaggtc ttactttatt
    23221 agaagtaata atagctgttt ttatactgac aatagtttta agtatttctt ataaagtgtt
    23281 caatggtata acatcagcag taaaaaagca acagattatt acagatgctc aagtaaatat
    23341 taatctaatt aataagtatc taaatagaga tttggaaaac tgtaaagagc taactaaaac
    23401 tggttcaggt aataattatg aatacaatat agagatgcca gataatgttg taaaatatga
    23461 agtttctata gaaactaaaa aaaatacaga ggtatattct gtaacgagaa tccaaaacaa
    23521 tacaattgat acagaaaatg aagttagaga agagataatc tataatcaac cactagttca
    23581 aaataataaa gaaatgaagg aaacaccatt taaaatagaa aagcaaactg gtaaatctat
    23641 ttatactgta agtatatact ataatgaatc ggtgcaagaa agtcataaaa atagcaattt
    23701 aaataacaaa acttatacat ttgatgttat gtcaagaata gggtaataag aatttattaa
    23761 aaaataagta ggttgataaa atatgagtaa aaagaatagt gaaagaggat tttccttaat
    23821 tgaagtgtta gtagctatgg ctattatggg aattgtacta tttgcatttt ttaatattat
    23881 caatactaat aacaaagcaa acactaaaaa tgacacagat ataacttcct taaactatgt
    23941 ccaaagtgaa atagaaaatc taagagaaaa gataaaaagt ggagaatttg attttgatag
    24001 tttagataaa ctagaagatg gaactgtcgt atatgaaaaa ttaatagata aatcaaaaaa
    24061 agttgtatat gataaagttc ttagtgaagg tgatgtgagc ttatatgata ctccatatga
    24121 aaagattaca acaataaaag atgaagatgg taatttaatc gacaaaggaa atattacaaa
    24181 taagataaag actattgtag aagataaaag tggtcagata tataaaatag ctgtaactgg
    24241 gaaaagcatg aatgactatt caagtaaaaa agaggttaaa attgtaactg aaatatttaa
    24301 agataaataa ttttacttgg gggtgagatt ttgaggaagt ggaataaatt taaaagtgaa
    24361 agaggggcag ctttagtttt agttttggtt gtagtagcat tgctttctat tgttggattg
    24421 atattttcaa atcagatagc taatagaata aaatctacaa agactactaa tgaaggtata
    24481 caagcaaagt atttagcaga gacatgtgtg gaaaatagta ttgataaagc atatgaaaaa
    24541 ttgtatgatg agcttgagaa aatggataat gagtttaaaa gtgaaaatca agaaaaatcc
    24601 atatctaggt caaaattaag aaatatatca gatgaagatt ttaataatca agatgaggaa
    24661 aatattgaag cagaaagact tggatatatg aataatataa atttctattt aaataaagct
    24721 tcttcagact tggaaaaagc tagtatggaa ttgaaaaagc tatatgattt agatatgttg
    24781 gactatagag acattgaata tgtagatgca aatattattt cacatagaaa ttctatctta
    24841 gaaatttgca agaattatac ttctggagat attagtaaaa taaatgaata tattctgaaa
    24901 gaagatatag actcaactac attgattgag gcaaagttag tgaataatga tattctttta
    24961 aaaaagtttt tagaagaaaa taaaattgaa aatgaacatt taaattcagc cttttcacat
    25021 acttataagg cattagataa catttctttg gcgatgcaaa atatgattga gtatagacat
    25081 acgtttcata tagatgagcc aaaagtagaa gtatctaatg ggattccaga ctctcaacaa
    25141 tattatgaac taatacaaaa tcctataata aatagtatgg agtatagctg gaatagtaaa
    25201 tgggatacat tagaaaacct attggaaatt ttaccaaatc aaactcaagg atttaattct
    25261 ttaagagttc atttgagaaa taatgtaaga aaatttgaga agttaagcga taatatatct
    25321 agtggtaaaa aaaatacagc aaaaaatttc ttaaagtata aagagttatt gtatgaaata
    25381 tcagaccaat gtaatcaact aaaaagtatg tcttatgaga aaatacctgt taaatatgat
    25441 aacatggctt tgataacaac ttttgactat atacaaaatg agcttttagc agagataaaa
    25501 tgcagattaa aagaattaaa accacaagaa attgataaaa cagaaggtat tactataaaa
    25561 ataccttttt ataaggctga ctacgacatg acaaaagaag gatggcctaa attaaaagaa
    25621 aatggttcag gggcggagtt aagcttaatg gtaactggtg ataaaaatgg aataaaagaa
    25681 gtagaagtta cagatggtaa aaaaaatatt attggtttag gtgttgaaga aaattcaaat
    25741 tcaaagtata aggttgacgc cattgtaaac tttaatctaa atatagatac aaatgtagtt
    25801 ggaaattatg atataaaaga taaaattttg attaatcatg atataagttc atacaaaaaa
    25861 gtaaactaaa tataagaagc aacttagtat aaataaataa taaaataatt ttattattta
    25921 ctactaaaaa aataagtaaa ctaatagaag atatggagag taaatattat gtatatttat
    25981 gatttattag agcaaggtat aagactaaac gcatcagata tacacataac agttggaaca
    26041 aatcctgtag ctagagtaaa aggtggattt gtaaaactaa gtgaacaaat attgacatca
    26101 gaagtaacaa tgcaaatggc aaaagacata gcaggggaat ctatgtttaa agtaatagaa
    26161 gaacatggag aagctgattt ttcagcttct ctaaaaacag gagaaagatt tagggtaaat
    26221 gcatataggc aaaaaggaaa ttacgcaata gcaataagga ctataacagc agaaattcca
    26281 acatttgaaa aattaggcct tccagagagt ataaaatctt ttactgaaaa gcataaagga
    26341 ttagttttag ttacaggtcc aacaggaagt ggtaaaagta caacacttgc aagtatgata
    26401 aatataataa atgaaaaaca acaaaaacat ataattacac ttgaagaccc gatagaatat
    26461 gtacatcatc ataaacaaag tttagtaaat caaagagaag taggaactga tacagaaagt
    26521 tttcattcag cccttagagc tattttgcgt caggacccag atgtaatact aataggagag
    26581 atgagagacc ctgaaactgt ttcaatagca ttaacagcag cagaaacagg tcatttagta
    26641 ttttcaacac tacatacagt aggggcagct aagacaattg atagaatagt agacatgttt
    26701 caaccatccc aacagcaaca aataaaaaca cagttatcta cagtttgtga aggtgtggta
    26761 tcacagcagt tacttcctac agcagatggg aaaggcagaa tagcagcaat tgaattgatg
    26821 tttgctactc cagcaataaa aaacctgata agagaaggca aaacatatca aattccaaac
    26881 atgatacaaa ctggagttaa agtaggtatg aaaactatgg accaagattt aatggaactt
    26941 tataaaaatg gaaagattac aaaagatatg gcacttagta ggtgtactga ccaagagttt
    27001 atgacaagaa tgataggtgg agtaaactac aatggatatt ataatcgtta atctatatgt
    27061 atttatagtg ggtatagtgt ttggaagttt ctttaacgtc tgtattttta ggattccaga
    27121 aggtatatct atttctagac caccttctca ttgtatgagt tgtaatacta gactaaagcc
    27181 aatagactta ataccagtgc taagctattt aatgagtggc agaaagtgta gatattgtta
    27241 tgagaaaatt tctagtagat atgctatagt ggaattaatt acaggacttt tatttttaac
    27301 agtatttatg ttatatggaa tatctacatc aactatttat tatttggttc ttgtgtcttt
    27361 actgattata attacattta tagatattga ccactttata ataccagata aaatattggt
    27421 ttttggagct atattttcta tgatttttaa ccttttattt aaagaaattc cagttaagga
    27481 ttcaattcta ggtgttttta tttgtggagg aagtgtctgg attatagttc ttttgattga
    27541 atttgtaata aaaaaagagt gtatgggtgg aggagatatc aaactatttg caatgctagg
    27601 tttttacatg ggtgttaaaa atggtctttt gacagcctta ctaagcgttt atgtgggagc
    27661 agtctatgga atctgtgtaa taattataag tagaataaaa ggaaaggaat ataattctgt
    27721 tataccatat ggacctttta tatctattgg agcattaata acaatcttat ctggaaagca
    27781 attacttgaa ctttattttt ctatggttat atagatgata gtcaagttaa ataaagtttt
    27841 aatttaaaaa aatgaattaa agtatttaat aaggacaata ttattattaa ccctattact
    27901 aaagtgctac aaactatgga gtatataata ataatttcct tattttttgc acatttgata
    27961 aataaatctc tatgtgtttt ttgtgataga gaaaagaaaa atttatataa tacgtttttt
    28021 atttacataa taactgtagt tttatctgta ttaatatatc taaaatttgg gattagtgta
    28081 gaatgtgtac aatatatggt tttaattcca tttttaataa ttatatccat tgtagattac
    28141 tacacaatat atgtatatga tataacaata gttagtggta taatagttca aggtataatt
    28201 tttttggcct ttttcaatat agttcaattt gattatataa atcatatatt tggactattg
    28261 ataggcttta ttatacctta tattattgct aaactaacaa aaggattagg ctatggagat
    28321 gtaggtctct atagcttatg ttgctttgca ttgggaaata attatgggtt ttatgtgata
    28381 gttttatcat ttattttagc gtttttgttt tttattattt tatacataac taaaatattt
    28441 aaagatgtag atataagaaa gcaaattcct tttacacctt ttatttcctt gtcaacaatt
    28501 ttaataatat ttacagagta taatatattt aatttttatt ataatttgtt atacaaattt
    28561 atataacagg aggtaagaaa tgtacgtagt agtagggtta ggtaatccag gaaagaagta
    28621 tgagaagact agacataacg ttggatttga tgttatagat attcttgcta aagaatacaa
    28681 tattagtgtt actaaaataa agcataaagc tttgattggt gaagggagag taggtactga
    28741 aaaagtatta ttagttaaac cacaaactta tatgaattta agtggagaaa cattaataga
    28801 tatatataaa tattataagg ttgatttaag taatatagta gtagtatatg atgatataga
    28861 tttagaagtt ggaaagatac gtataagaaa aaaaggaagt ggaggaactc ataatggcat
    28921 gaaatctata actaagtgcc taggttcaaa tgactttcca agagtgagag taggtgtatc
    28981 taaaccagaa gctggtcaag acttagcaga ctttgtactt tctagattta gaaaagaaga
    29041 atctgataac ataaatgaag cattagaaaa agcggcagat gctattgact caataataag
    29101 agaaaatata gatatgtcta tgaacaaata taatggatag tgagagggag atttaaatga
    29161 atgatgtttt tttatatcct ttgcaaaatt caaaagagta taaagatata ataaactgta
    29221 taaaaaatac aaaaggctca ttattggtta atggactttt accagtacaa aaacctcata
    29281 tatcgtattc tatatttaat gatttatcac gacaaatgat ttttataacg agtagtgatt
    29341 tagaagcaaa gaaagtatac gaagatttaa gtttttatat ggaagataaa gttgaatatt
    29401 taggttttca agatatttat ttttatcact tggatgcaaa ggacagaaac gaagaagcga
    29461 agaaattaaa agtattgtta aaactagcaa ataaagaaaa aatcatttta gtaacttcta
    29521 ttgaggcagt tttaagaaaa tacataccaa aacaagtttt gctcgatagt gtatctcatt
    29581 ataaggtggg agatagctta gacttagaaa agttaactga gaaattagtt tcattgggat
    29641 atgaaagagt ttcaaaaata gaggggtttg gtcaatttag tataagaggt ggaattattg
    29701 atgtattttc acttgaatat acaaatccaa ttcgtatgga attatttgat gatgagatag
    29761 attccatacg aacttttgac gtatattcac aaaaatcaat tgataaattg caacaattct
    29821 ctataactcc atctagggaa tttatttatc ccgaaaaaac tacagatgca ttagttaaat
    29881 taaaaaaaga aacaactaaa aatactgatg aaggtacatt tcaaaatata gattatatat
    29941 caagtaagac ttattttgaa ggtgtagaga actatataga ttatatttat ccagaagaaa
    30001 acaaaagtat atttacttat ttagcagatg atgcaatagt atttattaat gatataacga
    30061 gacttaaaga aagatgcgaa aattatataa atgagtttag agaaaattat aaattaaatc
    30121 ttgaaagagg tctggctatt aaaaaccaag gtaaattgtt atatcactat acagatttag
    30181 agtacttagt aaaagacaag tctgtggtat taaactcgct attgccaaag tctataaata
    30241 acttttctat aaaatctata ataaattttg aaagtagaga agttcctaca tttaatggaa
    30301 aggtagattt gcttgtagaa gaattaaatc ggctgaaata taatggatac aaaataattt
    30361 tagcaacaaa taccttagaa agagcaaata aattaggaaa agatttattg gacaaaggct
    30421 tagaaacgac catttctaaa gatagagata ttgaaattaa atcttcacaa gttataattg
    30481 tacctgcaca cataaacagt ggttttcagt ataaatctat aaagtttgtt gttataacag
    30541 ataacgaaat gataggtgtt tataaaagag cgtctaagac tagcaataaa aaagttaaaa
    30601 agggaaagaa aatagaatcc tttttagatt taagtgttgg agactatgta gtccatgaaa
    30661 atagtggtgt aggtagatac acaggaattg aacaaattac agtcaatgct attaaaaaag
    30721 attatatgaa aatagtatat caaggtggag ataatctata tgtgccaata gaccagatgg
    30781 acaaagtaca aaaatatatt ggtgcagaag tagaaaaagt taagttaaat aagcttggaa
    30841 caaatgagtg gacgaaagca aaagcaaaag ttaaaaaaga aattgaagat atgacaaaag
    30901 acttgattga gctttatgca aaacgtgaaa aaatacaagg gtataagttt tcaaaagata
    30961 caccttggca ggcagagttt gaatcgttgt ttccatatca agaaacagaa gaccaattaa
    31021 aggctataga agaaactaaa aaagatatgg aatcaagtaa ggtaatggat aggctagtat
    31081 gtggagatgt tggttatgga aaaacagagg tagctattag aagtattttt aaagcttgta
    31141 tggaccaaaa acaagttgct gtattggttc caacaacaat attggcccaa caacattata
    31201 atacattcaa agagaggttt gaaaattatc ctttgagagt agaagtttta agtaggttta
    31261 aaacaccaaa acaacaaaaa cagattattg aagatgctaa aaaaggatta gtagatattt
    31321 taattggaac acatagaata atatccaaag atataaattt accaaactta ggtcttgtag
    31381 tcattgatga agaacaaaga tttggagtaa aacataaaga agctttaaag aaaattaaat
    31441 ctacagtaga tgtacttaca ctttcagcaa ctcctatacc aagaacatta catatgtctt
    31501 taagtggaat aagagacatg agtgttatag aggaacctcc acaagaaaga catcctgtaa
    31561 taacatatgt tacagagagt aaggaaagcg taattcaaga tgagatagag agagaattaa
    31621 gtagaggtgg acaggtattt tttgtataca atagagttga acacattgaa gaaatggcaa
    31681 gtatgattca aaaactcgta ccagatgcta gagtggctgt tgcacatgga agaatgactt
    31741 ctaaatcatt agaaaatata atcctaggat ttttaaataa agattatgat gtactagtct
    31801 gtactacaat aattgaaact ggtatggaca tatcaaatgc aaatacaatg attatatatg
    31861 atgcagataa aatgggattg gctcaacttt accaattgag aggtagggtt ggaagaagtt
    31921 caagacaagg atatgcatat cttttgtatg aaaaagataa aactttaagt gagatagcgg
    31981 agaagagatt aaaagctatc agagaattta cagagtttgg ttctggtttt aaaatagcaa
    32041 tgagagacct tgaaataaga ggagcaggaa atattttagg ttctcaacaa catggtcata
    32101 tggcagttat aggttatgat ttgtacgtta aaatgcttaa tgatgcaatt aaaaaagtca
    32161 aaggtgaacc tattgtggag gaaattgatg ttgaaataga tttaagtgta aatgcataca
    32221 ttccagataa ttatatcaaa gatgagttga ttaaaattga aatgtataag aaaatagctt
    32281 ctattgaaaa caaagaagac atgttggata tacaagaaga acttgaggat agattctctg
    32341 atatacctaa accagtccag acacttctta caatagctta tataaaatcg ttatgtaaga
    32401 tattaaaaat agaaaagata agacaattaa aagatgaaat attattagtt ccaataacaa
    32461 agtatagaac taaacagaaa ataggttata atatagttac agaattagaa gaattattag
    32521 aaaagatgtg caaggtaaaa taacttgtaa atttaagaag ggatgtttta aatttgaaaa
    32581 aggtaataac attggtaata gctatgattt tggtagtatc tgtgactgct tgcagttcta
    32641 gtaaaggaga aactgtggca acagttgaag gtacaaagat atcatcagat gaatttaaaa
    32701 aaactatagc tctttataaa gattctatgg aacaaactta tggaaaagat atatgggata
    32761 aggaagttga aaagggtgta aaatacaagg acaagtttaa agatttaatt cttgaccaac
    32821 ttataactac agaagtaata tattctcaag ctaaaaaaga taacttactt cctaaaaaag
    32881 aggatgttga aaaaagcttt aaagaattaa aagacgctat gggtaaagat gaaaaatata
    32941 aagaacaact taagaaatta ggtatagatg atgagttttt aagagaccaa caagagaaag
    33001 atttagcaat gcaaaactat caaagtaact ttgctaaaaa gactaaaata tcagatgaag
    33061 aaatgaaaaa atactatgat acacataaag atgaatttaa aaaagatgag gtagaagctt
    33121 ctcatatttt attgaaaaca gtagatgata ataacaagcc actttcagat aaggaaaaag
    33181 ctgaagctaa gaaaaaggct gaagaggctc ttaaagaagt taaatcaggt gaggattttg
    33241 caaaagtagc aaaaaaatat tcacaagata cttcagcaag tgatggtggt aaattaggat
    33301 tcttttcaag gggtcaaatg gttgctgaat ttgaagatgc tgctttttct atgaaaaagg
    33361 gtgaagtatc tgatttagtt gaaactcaat atggatacca cataattaaa gtgacagaca
    33421 gaataaatga gcaaacatca tttgaagatg ctaaagaaac tataaaagac caactgttga
    33481 aaaataaata tcaagaacaa atagaaaaat taactaaaga agctaaggta gaaaaagatg
    33541 aaaaagtaat aaataaaatc acaatctaag acttaaaaaa agagtaatat gcttaaaaaa
    33601 gctatatctg gataagatat agcttttttt gtatattttt cttaacaatg gtaaaaataa
    33661 acttaaagta atttatggag ggattaaaaa atatgaaagc aacaggtata gttagaagaa
    33721 tagatgatct tggaagggta gttatcccaa aagagataag aaagacatta agaattagag
    33781 aaggagaccc tttagagata ttcacagcaa aagatggaga agtcatactt aaaaaatact
    33841 ctccaatagg tgaattaaat gaattttcgc aagaatatgc tgaaacatta gctgaagtac
    33901 taggatatgg agtgttgata acagacttag attctataat agctgtgtct aagcttccta
    33961 aaaaagatta taaagaaaaa agtataagtg atgagttaga agcaataata agtgaaagaa
    34021 cagtaaaata ttcaaaagat aaaaacattt taccattatt caaggaagac caaaaagaat
    34081 atactagtca agttatcatg cctataatta gtgcatctgg tgactgtata ggttcaattg
    34141 tagttgtttc aaaggataaa gatacaattg cagaaagtga agaaaaatct ttaaaaattg
    34201 ctgctaactt tttaggaaaa caagttcaat aattttatca taaagacctt ttacatgaag
    34261 gtcttttgaa gtttttgtgt aaaatattga atatagcatt aaaaatatgg tatattaaag
    34321 tttgttatat aaacagtatg gaggttatgt tatgaacaat aacaaaaata aagactcctt
    34381 tctaaaagga gcccttatac ttgggctagc aggtgtaatc gtaaaaataa tgggtggatt
    34441 ttttaggatt ccccttggaa acatgattgg ttcagaagga atgggatact atcaggctgc
    34501 ttatcctgtg tatactctat ttttaacatt agctactgct ggatttccaa cagctgtggc
    34561 aaaattggtt tcggaaaaag ttgcaattgg aaattttaaa ggtgcaaata agatatttaa
    34621 ggtatcacat acagttttat tcattaccgg tataatatca ttttgtatat tattttttgg
    34681 agccgactac atagtaacaa acgtaatgaa aaatccaggt gcgttatact ctatgaaagc
    34741 cattgctcca gcattactat ttgttcctgc aatgtctgca tatagaggat attttcaagg
    34801 gagacaagat atgactaaga ttgctgtgtc tcaagttgca gagcaatttt ttagagttgt
    34861 attaggtctt actttggctt acttgcttat gaagagtcta ggtcagactt ttggtgcagc
    34921 aggtgctata tcaggggcta caataggttc aatagcatct atgctttatt tggtgtttgc
    34981 ttatatgcta gggagaaaag agcgtagagc agagatagat gcaagccaaa gatttaaaga
    35041 tgaaagagtg tcttatattt ttaaaaagct tttaactgtt gccattccta taacaatagg
    35101 agcatcagta atgccacttg taaatatgat agataatgtt attgtaataa gaagacttat
    35161 ggaagcaggc tttacataca aagtggcaaa ttccatgttt ggtcaattaa caggtatggc
    35221 aatggccaca ataaatttac cagcagtcat aacaactgca atgagtatga gcttagttcc
    35281 agcaatttct aaagcatatg cattaggaaa taagtcaaaa gctagaaaag atactaagag
    35341 tgcagttaag gtaactttac ttatagtatt gccttgtgcg tttggtatgg cttctttagc
    35401 aataccaata atgggtcttt tatttccaca tgaaccatct tctgtaggaa ctattttgtt
    35461 tactcttact ccatgtgtat tatttttagg attaatacag acattgactg gtataattca
    35521 gggaatggga aaaccaatag tacctgtaat tgctttatgt gttggtatgc tatgtaaaat
    35581 tgtaataagt tatactttaa caggtatacc agatattaat gtactgggtt cagcatttgg
    35641 tacagttaca gcatactttg tggcagccat gattaactta ctatatgtta aaaaacacat
    35701 gaatgtaaac ttttctaaaa aagaatttat aataaagcca tttataacag ttatgacaat
    35761 gtttataatg gttaagttga gttatggtgc tttagttgga ttcttaggaa acagcatatc
    35821 tacaataatt gcaatatgtg ttggtggaat tgtatatgta gtagtaatac ttggaatagg
    35881 tggaattaaa aaagaagaga ttctaactat gccaaaaggt gataaattat ataaattgtt
    35941 aaaaaaagtt aaacttataa gataatatag gaatatatat gcaaataaag gaaatactat
    36001 acaaatagta aatctcctta tttgtataat aatataaaaa taaagtgaaa tttgctaata
    36061 ggagtgatgt tatgggtaag ataagtattg taggtcttgg accaggagac tattctctta
    36121 taagccaagg tgtgttagaa tctttaataa atagccctaa agtttttttt agaacagaga
    36181 aacatccaat aattgataaa cttaaagaaa ctatacaata cacttcttta gactactttt
    36241 atgaaaaaga ggaaaatttt gataatgttt atttgaaaat atctgaattt atcatagaag
    36301 aatcagcaaa gggtgaccta gtgtatgctg ttcctggtca tccaagagtt gcagagaaga
    36361 ctgtaagtat aatagagaga ttagccaatg aaaagcacat agcagtagaa atattgcctt
    36421 ctatgagctt tgtagacgct atgtttaact atttggctat agacccatct gagggattta
    36481 aactggtaga tgcttttgaa atagaaaatt catatataga cttggataca agtatgataa
    36541 taactcaggt atatgataaa tttatagcat ctaatgtaaa gttaaaactt atggaacatt
    36601 atgactataa tcaagaagta tgtatagtaa atggagctgg tgtcaaagat ttagaaagta
    36661 aaaagtttat aaagctttgt gaattagaca gaaatgaaaa cttttttgac tatttaacta
    36721 gcttatatat acccaaaagt tctaaaaaga tgtataatac agtacatgac ttagaaataa
    36781 tagttaatac acttagaagt ccatctggat gtgaatggga taaaaaacaa acacatcaat
    36841 cattaaaaaa ttctgttata gaggaagcat atgaactttg taatgcaatt gataataatg
    36901 atatagatga aatggtagaa gagttaggtg atgttctttt acaggttata ttccattgtc
    36961 agataggaaa tgaggagggc tactttgatt taaaagaagt agtaaatggc atatgtaaaa
    37021 aattaattca tagacatcct catatattta gtggtgcaaa attagatatg tctaagtttg
    37081 agaaaacttg ggaagaatta aagaaagaag agaaaggcga aagtactata acagatggcc
    37141 ttagaaggat accaaaacat ttaccagctc ttatgaaggc tagtaaagtt caacataaag
    37201 cagcacttgt cggctttgat tgggacaatg tagaagatgt atttaaaaaa attgaagaag
    37261 aatatcagga attacttgac gaacacaggg tcgggaatat aaaatacata aaggaagagc
    37321 taggcgattt attattttcg atagtaaatc tagctagatt tttagacata gactcaggag
    37381 aagccttgaa ctgtactaca gaaaaattca taaatagatt tgactttata gaaaaaagtg
    37441 caatgaactt gaataaaaaa ttagaagaca tgacacttga agagatggat gagttttgga
    37501 atcaggctaa aaaacaataa aattgtggaa ggaaatttgt caaaaatcta gaacttttat
    37561 cacaaagata gaagtctata ttataaacat ttttaggagg taaatatcgt gaataaagct
    37621 gaattagtat caaagatggc agaaaaaagt ggattaacaa agaaggaagc agaagctgcg
    37681 ttaaacgcat ttatgagttc tgttcaagat gcactagtaa ataatgaaaa agttcaatta
    37741 gttggatttg gaacatttga gacaagagaa agagctgcta gacaaggaag aaatccaaga
    37801 gatccagagc aagttataga tataccagct tctaaagcac cagttttcaa agctggaaaa
    37861 ggattaaagg atataataaa tggataatta gaaatcttgt gaaaaagtgt ggataaattt
    37921 gccacacttt ttctatagaa ccaaggaagg tgataattat gagattagat aaatttttaa
    37981 aagtatctag gattataaaa agaagaactg ttgctaaaga tgcatgtgat aaagggattg
    38041 tcactataaa tggaaaagtt gctaaatctt cttcagaggt gaatattgga gatacattag
    38101 aaatacaatt tggagagaag aagatgaagt ttaagataaa tgaagtaaaa gagcatgtat
    38161 taaaaaatga tgcaaaagaa atgtacaaaa ttttagaata aaattaatag tcctctagta
    38221 tattaatagt agtattattg aaatatttac tggggggata ttatggaaca gaacataact
    38281 ttgaaagata gatcaaagct ggttatttcg ggagtcgagc acatatactc ttttaatgac
    38341 aaaagagtag agctcaaaac ttctgtaggc gagatggtaa ttgaaggcga aaacctagat
    38401 atgagcaagc taagtataga cgaaaatata attagcatag atggaactat aaattctatg
    38461 gtatatgcaa agcctaaaaa gccacaagag agtttcttga aaaaggtatt tagatagatg
    38521 atacctttta ctcaagatgt aagcattttt tatgctacta tttatggtgg aatactcata
    38581 ggggtgttgt ttgattttta tagagggcta agagggaatt ttaaattcat caattatttt
    38641 gctataatct ttgatgtatt gttttggttt ctagctacgg ttataatatt cgtaacaata
    38701 aaccttacag aattttttga tttaagatat tatcattttg tggctttgtt tattggtttt
    38761 attttatact ataacaccat aagtaaaatt gttttatcaa ttatcaataa aataattcgt
    38821 tttgtgagaa attcttttaa aaaagttaca cattacatag tcagtttttt aaataatttg
    38881 tattacgtta taatctattc actacaccta ctatttgata ttatatttta tataccaaac
    38941 atatttatag caacaagaaa aagtataaaa agaaaatcaa ataaaaaatt aaagaacaag
    39001 aaaaaatcaa aacctaagaa gaaaaaaaat aaaactaaaa aaagggtgta ggaaaaatga
    39061 acttaagaaa aaaattttca ggacagatta ttgttatcag tttattttta ggaatatcaa
    39121 tttttagtat gatgacagga tttgtatttg agtatacaaa agctaaggaa tataaaaaag
    39181 aaatagcaag tctaaataaa caattaaaaa agactgaaat acaaataaac agcttaaaga
    39241 aagatgaaaa atcatatgaa ggagacttag aggatatagc aagaaaaaga ttgaatatgg
    39301 taaaaccaaa tgaaacagtt tatgtagaca taaatcgttg attaaaactt gctaataagt
    39361 ttacagatgt attgatttta aaatttaaga tttaataaaa aagtcattca gttaaggatg
    39421 gctttttgtt tttattgagg agggaatttc ataatgaaag taggagctat agatattggg
    39481 acaaactcaa tgagattatt gatagcagat tatagagatg ggaattttat aaacagaaaa
    39541 aaatttgtta atacaactag aattggttat ggtgtggata gtaaaggata tataaaagaa
    39601 gatgctatca aaagaaatat aaaagcatta aaagaatttt ctgataagtg taatgatgaa
    39661 aagtgtgaaa aaatttattg tatggggaca tctgctctta gagacagtaa aaatggagat
    39721 tactttgtta aattagctaa agaagaaact ggaatagatg ttgatatagt tacgggaata
    39781 gaagaatcaa atttgggctt taagggtgtt ttagaaggaa tacaaggtga tgatactatt
    39841 attgttatag atattggagg aggttcaaca gaatttattg taggtgattc agaaggaatt
    39901 aaatttaata aaagtgagaa tgtaggtgct cttaggatga cagagaggtt tttgaagcac
    39961 gatattattg aagatgaaga gtttgaagct atgagttcat ttatatatga tgagattgac
    40021 aaaacgttaa atcatataaa agacagagga ttaaaaactc ttataggtat aggaggtacg
    40081 attacatcat tatcagcaat gaatcaagaa ttagagattt actctatgga aaaaatacat
    40141 aatagtaaaa tacacaaaaa agaaatagaa gaaattctac aaaaattaat aaagatgaca
    40201 ttaagtgata aaaagaactt aaaagggcta caagaaaaac gtgcggatat tataactgct
    40261 ggtgtaaaaa tattgaatat aataatggaa aaattagaaa tagaagaaat aaccgtaagt
    40321 gaatacgata atttggaagg cttaatatgt caaaagtcaa aaaaaatgta ttaatatttt
    40381 ttgattttta aaaccgatta tgacaaaaaa aattactcct ctttgttaca attcttaata
    40441 taaacaaaag aggaggtttt tttatgcaaa gaagtgtagc agttgtgaag aataagactg
    40501 taaaaccaaa tgtcataaaa ttaataagcg gatatattga ttttactacg gcggcattcg
    40561 ctgtgatggg atttttactt agtaggtcta ttatagtaga ctctgtagct cctttaggaa
    40621 ttgccttttt tatatgtgca tcaaagattg ataagtacaa gatacctgta ttcatatcaa
    40681 cattattagg aatattgtta tcatctaatg attttggaaa tgtagcaaaa tatgctgtgt
    40741 gccttacaat atttatggct atgaataaaa aattaaaatt atcagattca attgctaagg
    40801 ttgcatttat aggtgcaata atactacttc caatctccat gggacaagct ttcttttcaa
    40861 ataggtatgt atatggtctt cttatagcag gagtagaagc aataataatg tttatatcta
    40921 tatatgtgtt ttcttatggg ataggtttaa tggccaatgt caacaatagg atgtctgtac
    40981 ggacagaaga agccatatca ataagtttac tgctaacctt tagcataatg ggaataggag
    41041 atatatctct gtttggaata tcaataagga ccattttatc gaccgtattg atattgctga
    41101 cctcaatagt aggtggagct accatgggag catctagtgg tgtaattatt ggtgtagcat
    41161 ccattttaaa taatatgaca tcagctgttt atatggggat atattctttt tcaggattaa
    41221 taagtggagc atttaataaa ataaataaat atttctgtat actaggatat attctaagtt
    41281 ggacaataat atacctatat acttctggta ttacttctaa tatgatgcaa ttaagagata
    41341 tactattagg ttgtttgata gtactagtat tgccagagag attatttaat aagattgaaa
    41401 aattaataaa aagtaatgtg gcttcaaatg aaatcgtata cgattatatt atgagaagca
    41461 aaaacctaac taatagcaga ttgaacagta tttataaaac ttatgatgac cttgcagata
    41521 catttgataa gataagagaa aaagacaaag tgctagacca aagagacata gctaatgtaa
    41581 tagatatgat tcataatgat gaatgtaaaa gttgtagtat gagaagaatg tgctgggagt
    41641 caagatttaa tcatacatac actatgatat ataacattct tgaaaaaatt gaggagaaag
    41701 gggagttaag tctaaacgat atacctaaaa attttagaaa ggaatgtatg aaaccagagt
    41761 caatagtgaa aatatcaaat cactattaca aaatgtttgt attagattat gattggagtg
    41821 tgaagttttc agaaagtaga aagttaatag caaatcagat aagaagtatt tcaaaatcca
    41881 ttaaatcctt atctcaagat ttagaaggcg acataatgct agatatagaa aaggaaaaaa
    41941 atatatatga acagttagaa agatatgata taacagttga taaagtaagt tacttaacaa
    42001 aaagtaacag tgagtttgaa atatcaatag aaaagaaaac ttgccatgat ggatgtatgt
    42061 gtgaagataa aattgtaaat ataatttcag acctagtagg ggaaaatatg tctgttagaa
    42121 aaataggatg tcactgttta ggaggtaaat gcaaggcaac attcgttaaa tctcaaaaat
    42181 ataaggctgt tacagaagtt tcagcaatgt caagggatgg gcatatcctt tgtggagaca
    42241 attacacata catggagata aatgatggta aatacatgat ggctataagt gatggtatgg
    42301 gaaagggtaa aaaagcatat gaagaatctt ctgccactat tgatatactg gaaaaaatga
    42361 tagatgcaaa aattaaagat gagatagtca ttgatactat aaacaatatg ttgttgttaa
    42421 aatcttcaga agagatgttt tcaactctgg atttgggaat tttagattta aaacgaggtt
    42481 gtttagaaac tataaagatg ggagcgtgtt ctacttatat aaaaagagag gatggagaag
    42541 ttgacttaat atcatcatca tcattacctg tgggtatatt atcagatgta aaaattgata
    42601 gaaaaaatgt aaaagttaag gaaggagact atgttataat ggtttctgat ggaattgtag
    42661 atgcaggaag aaataacaat ttaggagaca attggttaat atacttctta aagaatatag
    42721 agactacaaa tccaaaggaa atttcaaatt taatattgga tagagcatta gagttacaag
    42781 cgttacaaat cgaagatgac atgactgttt tagttacgaa aatatgtaca aaataaggtt
    42841 tacaagatta tgtgtataag atgtggataa cttgtgtata atatgttaat agacttttga
    42901 aaaaagtata gttattaaca aaaataacat ataaatcaac aaaatttgtt gataagtatg
    42961 gcagaaatgt taataaaatg taaaaaaaga aaaaacaaaa gctattatca aaagagattc
    43021 atgttgaaat aactaaattt ctaagtaaag aaaacctgtg aatatgttga ttattatgtg
    43081 gataactatt aattcgatat ttatttatat agtatgaata attgaataat atatctaaaa
    43141 aaagccatcc tattattaag taaacttatt gaaaggatgg ctttttatga atagaaaatt
    43201 gtgcaaagca ataatacctt tatttatttc tattttttgc atgttaataa taagtataaa
    43261 agctgaccaa ggatatcaaa agaataatga cagagcaatt actgtcatga ataataatga
    43321 tactgaagat gatgctgaaa aaaaacaaga aatagactat gaaaaaacat gttggaattt
    43381 gcaatctttg tttaaaagtg atgaccaatg gaaaaaagaa cttaagagtt ttgataaaga
    43441 tactaaagag ttgaaaaatt acataggtaa agtaacaaaa tcgccaacac atttgtcttt
    43501 tgccttgaac attaaagaaa aacttgatat aagactaaat aaactttctg cctatgtaaa
    43561 attaaaacaa gatactaata aaaattcgta taagtactta gatatgaatg atagtatgag
    43621 taaatcctat gggaattata tggggatttg ttcagattta gagttagaaa tactaaaatt
    43681 atcaaacaaa gattacaaga aaattatttc aaataaaaat ataaatagga aatatggtgc
    43741 atatttaaga gatataagaa ggaataaagt acattatttg gatgataaat cagaagatat
    43801 tctaagcaaa gtatcttcaa taagtagtct tccaagtcaa gtgtatgaat tatttagaaa
    43861 tatggataga aaaactaatt taacaccagc tcaatatgcg agtgaattag agtcatcaga
    43921 tagagaaaat agaaaaaaag cttatcaaga tgaacttata acttacaatg ataatataaa
    43981 tacaattgca ggtttaatta ctggtcaagt taaaaagaat attttttatt ctcaggaaag
    44041 aggttatgag tcttctttag aaatgtacct ggaagctgat aatgtagatg aaaaagtata
    44101 caataatttg ataaggactg taaatagtaa tatggacagt ctacacaaat atatagattt
    44161 aagaaaaaaa gtattaaaat tagataaggt tcattcatat gatatgtttg tgcctatggt
    44221 taaggctgtt gataataata ttaattatga aaaggcacaa agtatagtat attcagcgtt
    44281 gtcaccttta ggaaaagaat acaatgatgt agtatataaa gctttcaatg aaaaatggat
    44341 agatgtttat tcacatgaaa ataaagtaag tggtggatac tgcttatctg tttatgaaaa
    44401 tcatccatat atacttttaa attataataa ttcattaggt tcagtttcga ctttatcaca
    44461 tgagcttgga catgctgtat atgaatactt aagttctaag aatcagaatt attttaattc
    44521 aaatccgtct atatttactc acgaagttgc atctacaacg aatgaagcat tactttacga
    44581 gatgcttata aaagaagcta aaggcaatga tgaaaaagct tattatataa cacagtattt
    44641 agacttgata aaagatactc tttatacaca aactatgtat gctgaatttg aaaaaactat
    44701 acacgagtta gtagaaaaag gaaaaagtgt aaacgcatta gtattaaatg atgtttgggg
    44761 acaacttctt aaaaaatatt atggacaaag ctatgaatta gaccaattat ctaagggcgg
    44821 atgggctaga atcccacatt tttataatag tttctatgtc tataaatatg ctacaggttg
    44881 tagtgcagga gtaagttttg cccaagacat cctaaagaat ggttcagata actatatgag
    44941 ctttcttaaa aagggaagtt cagattatcc aataaacttg ttaaaatcaa gtggcataaa
    45001 tttaactaat acaaaaccca tagaaaatac aatcaaaaaa tttgatagtt tagtagcaga
    45061 acttgaaaag ctattataaa cattattgta atccacatgg taaatatatc gaattaatga
    45121 taaaatatac taaaagttta aagtcctatc aagataggac tttttattta ttaattagtg
    45181 ggagatgatt ctatgcaagt taaagttaaa aaggctgtaa taccagcagc aggtttagga
    45241 actagatttt tgccagctac aaaggcacaa ccaaaagaga tgttaccaat agttgataag
    45301 ccaactctac aatacataat agaagaagct gtggcctcag gaatagagga aatactaata
    45361 ataacaggta gaaataaaaa atctattgag gaccactttg ataaatctgt agaattggaa
    45421 cttgatttag agaaaaaagg taagaaagaa ttgttggagg tagtacaaaa catatctaac
    45481 atgattaaca tacattatat aagacaaaaa gagccaaaag ggttgggaga tgcaatttat
    45541 tgtgcgaggc attttatagg tgatgagccg tttgcagtaa tgttagggga tgatattgta
    45601 gataatgatg taccttgtct aaaacaacta acagatgcat atgaggaata tagaactaca
    45661 atattgggtg ttcaaaaggt aaatcaagaa gatactaata aatatgggat aatagaagca
    45721 aaaaatatag aaggtagagt ttacaaggta aaagacatgg tagaaaagcc agagtcagga
    45781 aaagctcctt caaatattgc tattttaggt agatatataa taactcctga aatctttgat
    45841 attcttaagg atttaccacc aggtaaaggt ggagaggtac aacttacaga tgctttaaaa
    45901 atattatcta agaaagaagc tatgtatgca tacaattttg aaggcaagag atatgatgtt
    45961 ggggacaagc taggattttt agaagctact gttgactttg ctctaaagaa agaagacctt
    46021 aaggaagatt ttataaaata tttgaaacat gtatgtagtg aatttgataa aaataacaat
    46081 gtgttataca ataatacaga tgaaaaaata gatttaattg aagtcaatga agctagagaa
    46141 cttgaaattc aaaaataatt aatgaaaggt gatgtatggt ggcaaaatca agtcagaaaa
    46201 gaattaatag aataatagct gttgggattg tgggaatcct cgtaataacg agtataacag
    46261 ctgttttgag tatgattgca actatgtaat aagataaatg acagcagttg atatacataa
    46321 atataaaaac catatataac ttgatgtagt caaaaactta caatatttta gttatatatg
    46381 gtttttagaa tttataaatg taaaaaaaaa tttttataat agttgcatat tttaaaggaa
    46441 aatattatta taagatataa atatagtgat aaatttataa aaacgtgcta agattggact
    46501 tgaaatacag taatatagta ggtaataatg ttttaaattt tatttattga ccataatata
    46561 taaataatga tattattaga cttaactgtt taaataaaac ttttcttttt catgccttca
    46621 atttccaaaa taagtatgta tataaatgaa tatatagatg aggaggattt ttttgatttt
    46681 agatgataaa gtattagaag gattaggtag taagacctta gttgagctac gagagatagc
    46741 taaagaatta aaaataaaat caattactac atataagaaa aatgagttaa ttgagattat
    46801 aaattccaaa agtaaaaatg aggaaaaagt aaacgaagag actaaagaaa aaaatgaaga
    46861 tattaaagaa gacataaaga atagaaaaga aaatatagag cgtgaaagta tagaaaaaga
    46921 agttgaatat aagagcccta ccaaatcata caacaagaaa gaaatagata cccaaaatat
    46981 ttcccaaaac caagaacata gaaatcaaag aaatgactat aataagacaa cgagtgtaaa
    47041 tgatagtaat aagtcatcaa atatatctaa tagaatggtc agaaataata ataaaaacta
    47101 ttatatgcct aaacaagttg atgaatctaa gatagtagat gaatttaaca catcaaaaga
    47161 ggatgaagta gtaggtgtac ttgaaattct gccagatgga tttggttttt taagaggttc
    47221 aaattatttg tctactgaag gagatgttta cgtttcacca tctcaaataa gaagatttaa
    47281 tatgaaaaca ggagacaaga ttaagggaat aactagacat ccaaaaagtg gagagaaatt
    47341 tagagcactt ttatatgttc aaaaaataaa tgatgaaaac cctgacacag ctatacaaag
    47401 aaatgcgttt gagacattaa caccaatatt tccagaagaa cgacttacac tggaaactaa
    47461 tagaaatgaa atagctacaa gaattataga tttaatttca cctataggaa aaggacaaag
    47521 aggattgata gtagcacctc caaaagctgg gaaaacagtt cttttaaaaa gcgttgcaaa
    47581 tagcatagct aaaaatcatc caaatgttga acttatagta ctccttatag atgaaagacc
    47641 agaggaagtt actgatatga aagaatctat tgaaggagac gttatatatt ctacttttga
    47701 ccaagtatca agtcatcatg tcaaggttgc agagatggtg ttaaatagag ctcaaagatt
    47761 agttgaacat ggtaaagatg tagtaatttt actagatagt ataactagat tggcaagggc
    47821 atataatctt acaatatctc ctacaggtag aactttatct ggaggtattg accctggtgc
    47881 acttcatgga cctaaaaagt tctttggtgc agcaaggaat attagacagg gtggttcttt
    47941 gacaatatta ggaacagcat tggttgaaac tggttctagg atggatgatg taatatttga
    48001 ggaatttaaa ggtacaggaa atatggagct acatttagat agaaaattag cagaaaaaag
    48061 aatattccct gcaatagata tttacaagtc tggtacaaga agagatgatt tattgcttga
    48121 tgatgaagaa aagactgctc tttggagatt aagaagagaa atgagtaata attcagtaat
    48181 ggaaattaca gataaagtca ttgaacttat aaagagaact aaggacaata aagagtttgt
    48241 aaaatcaata aagaatttgt aaaatcaata atacaaatgc aaatatggat tatataaact
    48301 aatagaaaaa tcacttgaat ggcataataa gttgtgctat aataatgtag ttgataatgt
    48361 aaaaagaaag ttaatataat taattttttt aaataaagag aagaggtgac tataatgcaa
    48421 aaggaaatac aaccaaaata caatccagtt gaagtgcgtt gtgcttgtgg gaacacattt
    48481 gttgctggtt caactaagga cgaaataaaa gttgaaatat gttcagagtg ccatccattc
    48541 tatacaggaa aacaaaagaa catagaaaaa ggtggaagaa tcgacaaatt caagaaaaga
    48601 ttcaaaatgg actaattttt ataaaagtgg agttggagga agccaactcc tattttatta
    48661 caaaaaaaca ttcatattct actttaaatt gtctttttgt attataataa taaatatgta
    48721 aaattgagta ttctaaatac aataaaatga ggtgagtgga tggcaaaaca agctgtaggt
    48781 gggcaagctg ttattgaagg tgtaatgatg cagtcaaaag acaaaagagc ggtagctgtt
    48841 agaaaaagtg acggagaaat agcactaaaa gaagatagaa taaaaagttg ggtaagagat
    48901 aagaatatag ataagatacc ttttgttaga ggttcttttg ttatgataga tacaatgatt
    48961 caaggcataa aaagcttaaa cttttcttca gagtttttta tggaagaagc tgaagaggat
    49021 aaatttgatt tatttataaa gaaaattttt aaagataagg caaatgatat aattataata
    49081 ttttcattgg tcatagcaat gttactttct gctggattat tcatttttat acctacattg
    49141 gtaggaggag ctttttctaa agtaatgcct aatgatttta tgcttaattt aatagaagga
    49201 atcataagga tagctatatt atttgcatat atagtattga tttcacgaag taaggatata
    49261 gaaagagtct ttcaatatca tggagcagaa cataaatcta tatattgcta tgaaaatgat
    49321 ttagaactta cagtagagaa tgctagaaaa tttaaaagat tacatcctag atgtggtaca
    49381 aatttcctat ttatagttat ggctgtatca ataatattgt ttgcattttt tggttggcca
    49441 aatccaattc ttagaatatt tatgagaatt atatgtgtgc ctatagtagc aggtttatct
    49501 tatgaggtaa ttagagttct tgggaaatat gataatggat ttactaaaat tattgcatat
    49561 ccaggaatga tgttgcaata ttttacaaca aaagagcctg atgatgagca attagaagtt
    49621 gctttggaag cattgaaagc agtagtagat tagagaaaag gatatttgtt atgacgataa
    49681 aagatataat tattaaatat tcagataaac taaaagatat aagtgatact ccaagattag
    49741 atacagagtt attgttacaa aaaacattgg gtgttgatag attatatatt cacttaaatc
    49801 tgaataaaga gctaactgaa gaacaaaaaa caaaatttat gggttttgca gaagaaagat
    49861 taaatggaag accaatagca tatatagttg aaaataggga gtttatggga ttagattttt
    49921 ttgttaagga aggcgtattg attccaagac cagatacaga aactttagta gaagaaataa
    49981 ttgaaatatg tagagaaaaa aaagatgtaa gtatattaga tataggaact ggttctggag
    50041 ctattacaat aagtttagca aagtacattg aaaattctaa aatcatgtct tttgatatat
    50101 cagagacagc attagaaata gctaaaaaaa atgctattat aaatgaagtt ggtgaaaaaa
    50161 taaagtatat taattctgat ttatttacag caataagtga tagtaacata aaattcgata
    50221 tcatagtttc aaatccacca tatattaaaa aacaagatat agaaactctg cacaaacaag
    50281 ttaaagatta tgaaccatat aatgctcttg aaggtggaga agatggatta gacttttata
    50341 gaagaataac agaacaaggt aagaaatatt taaataagtg tggaatatta gcatatgaag
    50401 ttggtcataa tcaagctgaa gatgtaataa atattatgaa aagtaatgga tataaaaaaa
    50461 tatacacaaa gaaggatatt caaggcattg atagagttgt tataggttac aatatatgat
    50521 ataatcaaca attgatatgt tacatttaat cgaggtgaaa gtatgttaaa aaaattagag
    50581 gtattggaag atacatataa agacttaagt gaaaaaatag gtgatccgga tgtaataaat
    50641 gatcaaaaag tttggcagaa atatataaaa gaacatgctg acttggaacc tattgttatg
    50701 aaatacagag agtataaaag cgttttagat agtataaaag aatcaaaaga aattttacaa
    50761 gaagagtcag atgaagaatt aagagaatta gctaaaatgg agttagctga gatggaagaa
    50821 aaagtagcac ctctggagga agaaataaaa atactattat tgcctaaaga ccctaatgat
    50881 gataagaacg ttatagttga aattagaggt ggagctggag gagatgaagc agctctattt
    50941 gctggtgatt tatttagaat gtactcaagg tatgctgaaa gaagaagatg gaaaatagaa
    51001 ttattgagtg caagtgatac tggagttggt ggatataaag aagtatcttt tatgataaaa
    51061 ggtaaaggtg cttattcaag attaaagtat gaatctggag ttcatagagt tcaaagaata
    51121 ccatcaactg aatctggtgg aagaatacac acatcaactt ctactgttgc agtattacca
    51181 gaagttgaag atgtagaggt tgaaataaat ccaaatgatt taagaataga tgttttccgt
    51241 tcttcaggta atggaggaca aagtgtcaat actactgact ctgctgttag ggtaacacat
    51301 atacctacag gtgaagtagt atcttgtcaa gatggaaaat cacaattaaa aaataaagag
    51361 caagctttaa aaatattaaa agctagactt tacgataaag cattggcaga acaacacaaa
    51421 gatatagcag cagaaagaaa aagccaagtc ggaacaggag acaggtcaga aagaataaga
    51481 acatataatt tccctcaagg tagaataagt gaccatagaa taaatttaac tttatataag
    51541 ttagatgcat tcttagatgg agatatagat gaaatgatag atgctttaat tactgttgac
    51601 caaacagaaa aaatgactgc aatataagta atgttttaaa gaagctgttt cttcctagaa
    51661 attattttct attttgaaac agctattttg cattttaggt catgttatca tattctattg
    51721 tatataattg ttatatacaa gtataggagg gttatatgat actaaaagta acaataattg
    51781 ggcttttggc aggtgttata gggacaggat taggaggagt aatatcagcc atttttaaaa
    51841 gagaggtcga taaatatcta agttttttta tgggattatc tggtggaata atgttggctg
    51901 tagtggtgtt tgatttaatg aaagaatcca tggacaaaat ggggataatc aatacagtta
    51961 tatttacgtt tgttggagct ctcataacta tgtacataaa aacaaaatta gatgtgtctg
    52021 gaaatatggc atctggatat cttatattca taagtatatt actacataat ttaccagaag
    52081 gactagccat tggttcttct tttatgtcaa ctgaaagttt aggtattaca cttgctatag
    52141 ttataggtct acataatata ccagaagggt tagctatggc attaggctta gtttgcaata
    52201 aaatgaaact aagtaaagtt attttgttta ctgtaatagc tggattacct atgggtctag
    52261 gaagtttttt aggtgtttat tttggaggag tatttacttc tcttatagga gtatttcttg
    52321 ctacagcagg aggtactatg atgtatgttg tattggaaga aatttttcca cattcaaaaa
    52381 gtgtgtattg tataattggt ttcttgttag gtacaatgat tgtaaattat atataaaatt
    52441 aaaatgaaag caggcataca aaagatgaaa actattataa gtaatattga tataaataat
    52501 attgattatg aggaaataaa aatacaagca aaacttctta gagaaggtaa aacagttatt
    52561 tttccaactg agacagtata tgggttgggt gcaaatgcat tagatgaaaa tgcagtaaag
    52621 aagatttatg aagctaaagg aagacctagt gacaatccat tgatagtcca tatatatgaa
    52681 aaagaagaag tatatgactt agccaaagat ataagtgata aagctaaact tgttatagaa
    52741 aagctttggc caggacctat aacaattata ttaaataaaa aagatataat tccatacaaa
    52801 actagtggag ggcttgatac agttgcaata aggatgcctt ctaatgttat tgccagggca
    52861 ataataaaag aagctggtat acctattgca gctccttctg caaatatctc aggtcgccca
    52921 tctccaacaa aagcaaaaca tgtatatgaa gagatgaatg gaagagtgga tggaatagtt
    52981 ttaggaggag acagtaattt tggattggag tcaactgtat tggatttaac agaagagact
    53041 cctatgatac tgagaccagg aagtataact aaagaagttt tagaggaact tcttggagaa
    53101 gtaaagcttg acccatcatt aagtaaaaaa gaagataatc aaaaagctaa agctcctgga
    53161 atgaaatata aacattattc tccaaatgca gatgtatata taatatctgg aaaaagagaa
    53221 aatgtagcaa caaagattaa tgatatgata aaatctaata gagacaaagg tctaaagaca
    53281 ggtgtaatgt gtattagtag aaaccgagag ttttatgatg gagaagtaat cgatttagga
    53341 aactctcttg aagaagttgc aagtaatctg tttgatgctc ttatagaaat ggacaaaaag
    53401 ggtgttgatg tcatatattc agaagagttc cctaagactg gagttggaca agcaattatg
    53461 aatagactac tgaagtcagc aggatacaaa gtgataaagg cttaagaggg agttgtaaaa
    53521 tatgaatata cttattgtgt gtacaggtaa tacttgtaga agtcctatgg ctgaggctat
    53581 actaaggaaa gcaattaaag aatctggaag aagtattgaa gaatattcta tatcatcagc
    53641 aggtatatct acagcaaatg gaatgggtgc ttctgaaaat tctatagaag tactaaaaga
    53701 aatcggtata gacttatcaa atcatagaag taaagtaatt actaaaaaat taattgatga
    53761 atcagatata atactaacaa tgactaaatc tcataaagaa attttagtac aagcagttcc
    53821 aaaatgcaaa gaaaaagtgt atacttttaa agggtttgca aataaaaatg aggaagatat
    53881 atctgaccca tttggtggaa atttggatat ttatagaagc actatgagag aaataatgta
    53941 ttctgtcaac gaaatagtaa aaaaaatatg aaacggagga taaacaaatg aagataggat
    54001 taggctgtga ccatggagga tataatttaa aaaaagaaat aataagctat ttagaaagta
    54061 aaggcataga atgtgtagat tatggaacta ataatgctac agattctgtt gattatcctg
    54121 tttatggaga aatagttgca aattcagtta taaataaaga agtagattat ggaatattat
    54181 gttgtggtac agggatagga atatctttag ctgctaataa ggttcctggg ataagatgtg
    54241 cagttgtatc agatgtattt tctgcaaaaa tgtcaaaagc acataatgat gcaaatatgt
    54301 tatcattagg cgaaagagtt ttaggaaaag gtctagcact tgagatagtt gaagcatgga
    54361 ttaatacgga ttttgaaggt gatagacatg ctagaagagt aaacatgatt aaatcaatag
    54421 aagaaaaaca taataaataa taaaaattac agttttatca ggaggaatta tcaaaatgag
    54481 caaagtggta gagacaaacc atccattaat acaacataaa ttaactttaa tgagagataa
    54541 aaatactggt tctaaagact ttagagagct tttaactgaa attgcaatgt taatggggta
    54601 tgaaataaca aaagatatac cattaaaaga tgtagaaata gaaactccta ttcaaaaaac
    54661 atcatctaaa gtagttgcag gtaaaaaatt agcaataatt ccaattttaa gagcaggtct
    54721 tggaatggta gatggattag tgagcttaat gccagcagca aaagttggac atgtaggact
    54781 atatagagac ccagaaacat taaaaccagt tgaatattat tgtaagcttc ctcaagacat
    54841 aggagaaaga gatataatag tagttgaccc tatgcttgca acagggggtt ctgctgtagc
    54901 agctatagat ttacttaagt ctaaaggagc taaaagtata aaattagcta atttagtagc
    54961 tgcaccagaa ggtatagcag aagtgcaaaa atatcatgat gatgttgata tatatgttgc
    55021 atcagttgac gaaagattaa atgaacatgg atatataatt cctggtcttg gtgatgctgg
    55081 agatagattg tttggaacta aataaaaata aataactata aatatttatt agggactttc
    55141 ttatatagtg tatataattt tatgtaagaa ggttcttttt tatatatttt ttctcaaaat
    55201 aggaattatt gaattgataa atttctcgaa ttgagaataa aaagttttta ccaattaaaa
    55261 aagattgctt attttacaag gttttataga atagtagtct attttaatgt ggcatgttag
    55321 ttgcttacga ttaagattat aggtattaag tgtattagtt aaattgagaa ttgaatatta
    55381 ataatattat tgggagtggt aatacatgaa gaattgtata gtagtaagag gggctggaga
    55441 tttagcaaca gcagttattc ataaattaca caacagtggt tttaaagtag ttgcgttaga
    55501 aatagaagag ccattagcta taagaaggca aatttctttt tgtgaggctg tttatgaaaa
    55561 aaaagtaact gttgaaggtg tagaatgtat attttgcaat actgaaaaag aaatagaaga
    55621 agttttatct caacataaag tagccttgat tatagaccca aaaggagaaa aattatctaa
    55681 attaaatcca atcgttttgg ttgatggaat actcgctaag aaaaacttag gtacaactaa
    55741 agatatggca ccacttacaa tagctttagg tcctggattt tgtgcaggag aagatgtaga
    55801 tgttgttata gagacaatga gaggtcataa tctaggaaga ataataacta atggtagagc
    55861 agaaaaaaat acaggagtac caggagcaat aaaaggtttt tctaaagaga gagttatgca
    55921 ttcacagtat gatggcataa tggaaaatgt atgtaagata ggtgatatag ttgaaaaggg
    55981 agacctactt tgctatatta ataattatga aaaaaaacat gaagttagag caacaatatc
    56041 tggagtcctt agaggtttat taaaggatgg agcaaaggta aacaaaaatt taaaaatact
    56101 agacatagac cctagaaaag aagaagttaa gaatgctttt acaatatcag ataaagctag
    56161 atgtatagct ggaggggttt tagaagccat actaaacaat gggatttatc cccaaaatta
    56221 atttatatta gataaagcta ttaatatttt attaagatga tataataaat ttaatagatt
    56281 aaagagagca tatgacgggt atttaaaatt taaatggtaa aaatgctctt tttttattgg
    56341 acattcaatt aacatgtgta tataatataa aaataagtat ttatttaggt tgaaatctaa
    56401 taaatatata ataccagtac ataagcaaag aggagaaaag taaattatga gaccatcatg
    56461 ggatgaatac ttcatggaaa ttgcagaagt agtgaaaaag cgttcaactt gtattagaag
    56521 acaagtagga gctgtaattg taagagacaa acaaatatta acaactggat ataatggttc
    56581 tccgagaaat cttgaacact gtgaaaatat tggatgtaag aggcaggaat taaatattcc
    56641 ctcaggagaa aggcatgaat tatgcagagc attacatgct gaacaaaatg cgataataca
    56701 agctgctcat aatggtatta gtgtagatgg ggcaactttg tatgttacta caagaccttg
    56761 tgttttgtgt gcaaaaatgt gcataaatgc aggtatattg aaaattgttt atgaaggtga
    56821 ttatccagat gatatgtcta cagaattatt aaaagaagca ggaatagaac tcataaaatt
    56881 ttagtggata aaatttatat gacaataaat gtcaaattat gacactgaaa tacaatggta
    56941 tcaaatgatt aaaagaaagt atttgaaaaa tcatcagatt agtagtaaga tatattttat
    57001 aattaaataa atttatgaga tagattaatt acatgttatt tattttataa taatgaggga
    57061 tactatttat gagtaaaaga aatacacata cagaaattgc aaagttgttt agtttaataa
    57121 gccaaatagg attgatgatt gtaatatcaa tattaggatg tactttctta ggaaaatttt
    57181 tagatagtaa attgaatact actccagttc tgacaataat atttttattg ttgggtgttg
    57241 gaggtgcttt tagtggtgtt tataaaactc taatagtata tacacgaagg aagtgatata
    57301 ttggatataa aattacttaa ccaggtaagg tcagtaacaa aaggtatagt aatatttgat
    57361 gtagttttaa ttgtgctaat gttgataaca tcaacattaa gtaaaccagt attattagga
    57421 cttatatttg gctctataat agctatgtta aattttagat tattggcgat atcactggaa
    57481 aaatcagtga catttcctgc tgggaaggca caagcttatg catcagcaca atatgctgtt
    57541 agattattta taatggcagt tgttctaatt gcatcagcta cagtaccaca tttaaatata
    57601 atcggaacag cattgggatt attgagcaca aagtttgtca tattatcaaa aaactttata
    57661 aataaattaa agaggaagga ggcataagta tgaatcaagc gcattattat ctcataggaa
    57721 actttaagat tagtgaaaca atactagtaa gttggggaat aatggccttt ttagcgatag
    57781 catcttattt tttgactaga aacttaaaaa aagttccaac tagtaaagtt caaatattct
    57841 tagaatttgc agtaggtggt cttgctaaaa tggttacaga tgttatgggt aaaaatacag
    57901 taaaaagaat gccatatatg ataccataca taggaagctt gtttttattt ttcgcatgtt
    57961 caaatttaag cccattactt ggattaaggt caccaacaac cgacttagat acaactgctg
    58021 catgggcaat gattacattt tttatgattt attttgcagg agttaaattt aaggggttat
    58081 catactttaa agaattggct caaccacttg ccataatgtt accaatcaat attataggtg
    58141 agttagctag accaatatca ttaagtttcc gtccatttgg gaatatttta ggtggaacaa
    58201 taataatggg tctgttttat caacttttag gttttatttc tagtttaata ccaggattat
    58261 caataccaat tggagaaata ttaataccag tcccattaca cttgtacttt gacttatttg
    58321 caggattgtt acaagcatat atatttataa tgttaacaat ggtatttata tcaagtgcag
    58381 gagagttaga ttaagaaatc aacttatatt aagataaaaa taaaataatt taaaaaatta
    58441 aagttttaag gaggaaataa ttatggaaac agcaatagta gcggcagcat cagcaatagg
    58501 agcagggata gcagttgcaa caggtatagg tgcaggtata ggtcaaggta tagcagcagc
    58561 taaagctgca gaagcagtag gaaaccagcc agaagctaaa ggtgatataa catcaacttt
    58621 attactagga gttgctatag cagagtcttc agcaatatat ggtttggtta tatcaataat
    58681 acttctattc gtaaatccat tctttaaata cttaggaatg tagatttagt ccttaaatat
    58741 tttgagatag gtttataggt aaatcatctc aaacttataa tcatgagatg aactttatca
    58801 tctcgtgatt ataagtagtt atgatttatg gaaaggagga taattatgga aaaagcacta
    58861 gtaggtataa cgtgggaatt tgtgttccaa attgttaata cttttataat atttttactt
    58921 ctgagaaagc ttttattcaa gccagtatta aatataattg aatcaagaga aaatgatatt
    58981 aaaagtgatt tggtagaagg cgaaaaagca aaaaatgaag gtttagctct gaaaaaagaa
    59041 tatgagtcaa aaatcaattt tgccaaagat gaaggtcaag aaatcataaa acaagcaact
    59101 ataagagcag agcagaaatc agatgatata gtaaatactg caaagaaaga tgctcttgat
    59161 ataaaagaaa aagcaaacaa agatatagaa caagaaagac aaaaggttat aaatgaaatc
    59221 aaaaatgata tatctaacat agcactactt gcagcatcta aagttattga aaaagattta
    59281 gataaatcta aacatgaaga attgatagaa aactttataa aagaggtagg ggaagctaaa
    59341 tgataaatgt aatagcaaat agatatgccg aagccctatt tcaattagga gaagaagaaa
    59401 attcaactga tgtcttattc aaagaactag aaaaagtagt agatatgatg actaaagtta
    59461 gtaaagattt ctacaaagtt ttaaaatcac cattagtttc aaaatcagaa aagaaaaact
    59521 tggtagaaat tatttttagt aaagaagtaa gttctaacat aaaaaacttt ttaaaagttt
    59581 tagtagataa agatagaata tcatatttag aagatatcga attagcttat aaagaattat
    59641 taaataagaa aaacaatgta attgatggag tagcaataag tgctattcca atgagtgaaa
    59701 cagatattaa agaacttgaa gtcaaacttt caaataaata caacaaaaat gttactatag
    59761 aaaatgttgt tgacaagact atactagggg gagttctagt tagaattgga aatgaacaaa
    59821 tagatggcac tgtgaaaact cgtcttgata agatgaaaga aaagctatct gaagtaatat
    59881 cttaagaatg gcggtgaacc catgaactta aaacctgaag aaataagttc tataattaaa
    59941 cagcaaataa aaaattatga gaataaagtt gagttgacag atacaggtag tgttttaaca
    60001 gttggggatg gtatagcgag tgtatatgga ttagaaaaag ctatgtctgg tgagttgcta
    60061 gagttcccag gtgaaatata tggtatggca cttaacttgg aagaagaagt agttggagct
    60121 gttatccttg gagatgacag tgaaataaaa gaaggcgata tagttaagag aactggaaga
    60181 atagttgaag ttccagttgg agaagcttta ataggtagag ttgtaaactc actaggtcag
    60241 ccaatagatg gaaaaggacc tatagcgtac acaaaaacta gaccagttga atctgaggca
    60301 cctggtataa tagatagaag gtctgtttat gaaccattac aaacaggaat caaatcaata
    60361 gattcaatga taccaattgg tagaggtcaa agagagttaa taataggtga cagacaaact
    60421 ggtaaaacat ctatagttat tgacactata ctaaatcaaa aaggaaaaga tgttatctgt
    60481 atatatgtag caattggaca aaaacgttct actatagctc aattagttag ctctttagaa
    60541 aaaggtggag ctttggatta tacaatagta gtttcagcaa cagcatctga atcagcacca
    60601 cttcaatata tagcaccata tgctggagca gctatgggtg aagaatttat gtacaatggg
    60661 aaacatgtac ttatagtgta tgatgattta agtaaacaag ctgttgcata tagagaaatg
    60721 tcattgttac ttagaagacc accaggacgt gaggcatatc ctggagatgt attctattta
    60781 cattcaagat tacttgaaag agcagctaag ttatctgatg aattgggtgg aggttcaatg
    60841 actgccttgc ctataataga aactcaagct ggtgacgttt ctgcatatat accaacaaat
    60901 gttatatcta taacagatgg tcaaatatac ttacaaccag agttattcta ttcaggtgta
    60961 agaccagcag ttgaccctgg tatatcagta tcaagggttg gtggttctgc gcaaattaaa
    61021 gcaatgaaaa aagttgcagg aacattaaaa cttgcatatt cacaatatag agaacttgca
    61081 gcattctcac agtttggttc tgacttagat gaagatacta aaaagagact tgctcaaggt
    61141 gaaagaatcg ttgagatatt aaaacaaggt gagcatcaac caataaaagt tgaaaatcaa
    61201 gttatgataa tatatgctgt tataaataat catttagagg atattccgat agataatata
    61261 gctagatttg aatcagaatt gtatgcattt gtagataata attatccgga aatatcaaga
    61321 aaaatattag gtggagaaga cttcactcat gatttaacag atgcaataaa tgaatttaaa
    61381 gaaaagtttg ttgttgaagt ataatccttt ttaaaaaagg aggtaaatta attggcagga
    61441 gctggaatca aagcaatcaa aacacgtatg ggaactgttg aaagtacaaa gcaaataact
    61501 aaagcaatgg aattagttgc atcttctaag cttagaaaag caaaagaaaa ggctgaagat
    61561 tcaagagaat attttaatac tttatacaat acagtaaaag atattgccca aacaacacgt
    61621 ggaattagaa atgaattttt aaaagaaaga gaagtaaaaa ataaatgcta tgtagtagta
    61681 gctggagaca gaggacttgc aggtggatac aattctaatg tattaaagga atctgtctct
    61741 catatgaaag gtaaaaagga aacagtgatt actgttggaa agaaagcaca tgaatttttt
    61801 tctaaaagaa actacaatat agcaaaagat ataaatagtg ttgaagcttg tggatatgaa
    61861 gatgcgacag aaatttcaca aactattatt gacctgtata aaaaaggtga agtagatgaa
    61921 gtttatattg cttatacaaa atttaaatct gctttatcac aagaagttga aattttgaaa
    61981 ttgcttccat tagtctttga aaaagataca gacgaacaag gaatggcagt aaaagaagaa
    62041 aaacaaaata gatcaaaagt acaatatata ccatcagcag aagcagtttt aagttatatt
    62101 gtacctcagt ttatatcagg tacagtatat ggtggaattt tggaatcttt tgcatcagag
    62161 caaggtgcta gaagaactgc aatggaatca gccacagaca atgcgaatga aatgctatct
    62221 aatttagagt taagttataa cagagcaaga cagtcagctg taactcaaga aataacagag
    62281 atagttggtg gagttgaagc tctaaaatag ggaggttagg aaatggcaaa tgtaggtaaa
    62341 gtagtacaga ttgtaggagc tgtactagat gttaagtttg atagtgaaca aagcctacct
    62401 aacttattaa atgcgttagt aataaagtta ggagataaag aaatagttgc agaagttgct
    62461 caacatatag gtgatgatac tgtaagatgt atagctatga gtgcaacaga cggacttgta
    62521 agaggtatgg aagttgtaga tacaggaggg ccaataagtg ttcctgttgg agatgaaact
    62581 ttaggtagaa tattcaatgt tcttggaaaa ccagttgatg ggaaaccagc tccaaaatct
    62641 gcacctaaat tacctataca tagaccagca ccggcgtatg atgaactaga aactacagca
    62701 gagatactag aaactggtat taaggtagtt gacttactag ctccatatct aaaaggtgga
    62761 aaaataggtc tatttggagg agcaggagta ggaaagacag ttctaataca agagcttata
    62821 aacaatattg ctaagcaaca tggtggtatt tctgtatttt caggagtagg agaaagaaca
    62881 agagaaggta acgaccttta tggcgaaatg agtgagtctg gagttataaa taaaacagct
    62941 ctagtatttg gtcaaatgaa tgaaccacct ggagcgagaa tgagagttgc tttaactgga
    63001 cttacaatgg cagaacattt tagagatgag caaggacaag acgttttact tttcgttgat
    63061 aatatattcc gtttcacaca agctggttca gaagtttcag cacttctagg acgtatgcca
    63121 tcagctgttg gttatcagcc aacattagct actgaaatgg gtgcacttca agagagaata
    63181 acatcaacta agaaaggttc aataacatct gttcaagcag tatatgtacc agcagatgac
    63241 ttaactgacc cagcaccagc tacaacattc tctcacttag atgcaaaaac agttttatca
    63301 agacaaatat catcactggg tatatatcca gcagttgacc ctcttgaatc tacatcaagg
    63361 attcttgacc ctagtatagt aggaaaagaa cattatgaag tagctagagg tgtacaatca
    63421 atacttcaaa gatataaaga acttcaagat ataattgcta tacttggtat ggatgaatta
    63481 tctgatgaag ataagttaat agttgcacgt gctagaaaga tacaaagatt cttatctcaa
    63541 tcattcacag ttgctgagca atttacagga aatccaggtc aatatgttcc tgttaaagag
    63601 actgtaagag gatttaaaga aattcttgaa gggaaacatg atgacttacc agagtcagca
    63661 ttcttatttg taggaacaat agaagacgct gttagaaaag cgaagggaag tatgtagtca
    63721 tggccagtga attttcattg aaagttgtaa ctccaagtaa tactttttat gatggagatg
    63781 ttgaaatgat agtagttaga actacaaaag gagacaaagg ggtgttgaaa ggtcatatac
    63841 catttgtttc aggtatacct gtaggaactt taaagataaa aaaagaggga agttttaaag
    63901 aggcgaaaat agcagaagga ttcattaatg taactcaaga taaaactgtc atattgacag
    63961 aatctgctga gtggatttaa taaattaatt tagagtataa aaagtcattt tgaccaaaaa
    64021 aacttttggg taaaatggct tttttgtgtt ttggtgttaa taaaataacg ataaaattgt
    64081 aagtttttag tgataataaa ttcacaaaat gtttaaattt actgccaaat tatgttgtaa
    64141 ttaataagat aaagaagatt tagatagctt ttatgtaagt gaaaaagtct tcatataaga
    64201 aatacatata aaatttttat aaaatttaaa aaatttgctt agttgtgtag tataataaat
    64261 aatataatag atttgtatat aatattcatt acaaataatt ttaaaagtta cagttttatt
    64321 ataatgatta ttaaattaag ataagtaaca taaactaaat aataatgtgt attatcgata
    64381 gtaaatttat aaattcatta ttaattattg aaagaagtat tataaatttt ctggaggaac
    64441 aatgaatata tttgatattg gcgtagatat aattgaaatt gatagaataa gaaaggctgt
    64501 agacaaaaat aataggtttt tggagaagat atttacagat agagaaatag agtattttaa
    64561 ttctaagaat tttaaagcag aaagtatagc aggtaatttc gcagccaagg aagctataag
    64621 taaatctata ggtacaggaa ttagagtgtt taattttaaa gatatagaag ttctcagaga
    64681 tgaaatggga aaaccaatag taaaaactta taataatcta gcaaaaatgt gcatagatta
    64741 taatgtttta gaaataaaag tttcaatatc acattcaaaa gattatgcta tagcaaatgc
    64801 gataaccata attaaggact aaggagtgat tacattatga tggataaaac agcaaaagaa
    64861 ataatgacaa cagatgtaat agtagcaaaa caagatgata gtatagcaga tgtagctaat
    64921 atgctaattg cagaaaaaat aggaggtctg ccagttgtag attcagagaa tagagttgtt
    64981 ggaataattt ctgaaacaga tattcttaaa aaagaaaaat atatagaagc acctctctat
    65041 ataaatctat tacaaggatt gattttctta gatgacttaa aaaaagttga aaaagatata
    65101 aaacaagtag ctgcttataa ggttggagaa cttatgtcta aagatataat aaaggttcat
    65161 gaagatgata agtttgatga tgtagctaat gtaatgatta aaaaatcaat aaacagagtt
    65221 cctgtagtag atgatgacaa caaacttaaa ggtataattt gtagatatga tataataaaa
    65281 gctttataca atgaataaac tgaagtagga ggtgtgatgt gagaaaaaag tggaccatag
    65341 tatgtattat gttcttggca ctggttataa ttgtaatagg atgccaaaaa cgacagtcca
    65401 caaaagaaga agtatacaaa gattttcaaa agcagatttc tgatatgaat tattacagtt
    65461 gtaaagctga agtagaagta gttggaaata aaagtccaca caattatgtt ttaattcata
    65521 cttataaaaa aaccgataac tataaattag aagtaatttc cccaaaacat ttaaaaggga
    65581 aaagtataga atatcaaggt gataaaatat tagttaaaaa tccaaagatt agtgatgtag
    65641 tggagttacc taatacagga aaaaataatc aatatctttt tgtaggagac tttataaaaa
    65701 actatcttca aaatgaggag atgaaagtaa agttatccaa aggacatttg gttttagaga
    65761 cttttattcc tggggataat aaatatttta acaagcaagt attatatgta aatgctgaca
    65821 caaaaaatcc tgaaaaaatg gaagtgttag ataaagaggg agtgccaaga tttacagtaa
    65881 aatacaaaga ttttgaatac agaaactaag ggggactaaa gacatgcaaa aaataacagt
    65941 gcctacatgg gcagagataa atctagataa cttaagattt aacttaaata atattaaaaa
    66001 tttattagaa gaagatatta agatttgtgg agtaataaaa gctgatgcat atggacatgg
    66061 tgcagtagaa gttgcaaaat tgctagaaaa agaaaaagta gattacttag cagtagcaag
    66121 aactgctgaa ggaattgaac ttagacaaaa tggcataaca cttcctattt tgaacttggg
    66181 atatactcca gacgaagctt ttgaagattc tataaaaaat aaaataacta tgacagttta
    66241 ttctttagaa acagcacaaa agataaatga aattgcaaaa tctttaggag aaaaagcctg
    66301 tgttcatgtt aaaatagact cagggatgac tagaataggt ttccaaccta atgaggagtc
    66361 agtacaggaa ataatagaat taaataaatt agaatatata gatttagaag gtatgtttac
    66421 tcattttgct acagctgatg aagtaagtaa agagtacact tataaacaag ctaataatta
    66481 taaatttatg tctgataaat tagatgaggc tggtgtaaaa atagctataa aacatgtatc
    66541 aaacagtgca gctattatgg attgccctga tttaagatta aatatggtaa gagcaggaat
    66601 aatattatat ggtcattatc catctgatga tgtatttaaa gatagattag aattaagacc
    66661 agccatgaaa ttaaaatcaa aaatcggaca tataaaacaa gttgaaccag gtgtaggaat
    66721 aagttatgga ctaaaataca caactacagg taaagaaaca atagctacag ttccaatagg
    66781 atacgcagat ggatttacta gaatccaaaa aaatccaaag gttcttatta agggagaagt
    66841 gtttgatgta gttggtagaa tatgtatgga tcaaataatg gttagaattg acaaagatat
    66901 agacataaaa gttggagatg aggttatact atttggagaa ggcgaagtta cagctgagcg
    66961 tatagctaaa gacttaggaa ctataaacta tgaagtgtta tgtatgatat caagaagagt
    67021 tgaccgtgtt tatatggaaa ataatgagct tgtacaaata aacagttatt tgctaaaata
    67081 aaaccatgat attataatct gggaggcgat tttgtgtacg ataagagtaa agaaaaaata
    67141 gaagttactt tacctgactc tctgatttct gaagttgata gtatagttca gatggagaac
    67201 agtaacagag aagattttgc aaaagctgct tttcagttct atataactca gaaaaagaaa
    67261 attcatatta aagaatctat gaaaacgggt tatagagaaa tgggtcaaat taatctgtca
    67321 ctagctgaat taggtatgac agttgaagaa gtatcttctg atgataaaaa tgaagggaag
    67381 attgcccaag gtgagtacta attgtgaaat aaagcgtggt gatttatatt acgctgattt
    67441 aagtccagtt gttggttcag agcagggagg cgttagacct gttctgataa tacaaaatga
    67501 tgtaggtaat aaatatagtc ctacagttat agtatcagct ataacatctc agattaataa
    67561 agccaagtta cctactcatg tagagataag ttccaatgaa tatgggctta ataaagactc
    67621 agtaattctt ttagagcaaa ttagaactat cgataaaaag agactgaggg aaaaaattgg
    67681 ttgtctagat gaaaatatga tgataaaagt agatgatgga cttcaaataa gtttaggatt
    67741 gtttactttt taaagaggga tttgtctttt attagacaat cccttttttc ttttaaatat
    67801 ctcttaaggt ttaattttaa tgtactatgt taataaaatt aaaaataacg caaaaaaata
    67861 gcataaaagt gatataataa tatggaattg atataaaatt acgaactcaa gggaggtaat
    67921 tgcatgagag accataaagg attaaatgag attgcgcgta tcataagaag agatatagtt
    67981 tcaatgatac atagagccaa atctggacat ccaggagggt cattatcagt agttgaaata
    68041 cttactgcgt tatactttga tgaaatgaat gtggattctt caaatcctaa aatggaggat
    68101 agagatagat ttgtattatc aaaaggacat gctgcaccag cattatatgc tacattggct
    68161 gaaaaaggtt attttgataa ggaagaactg aatggattaa ggaagatagg aaggatgtta
    68221 caaggtcatc cagacatgaa aggcacgcca ggtgtggaaa tatctactgg ttctttaggt
    68281 caaggttttt cagttgcatg tggtatggct atggcatcaa agttggataa tgctccgtgg
    68341 aatgtgtata ctttgcttgg tgatggtgag gttcaagaag gaattgtttg ggaagctgct
    68401 atgagtgctg cacattataa acttgataat ttaattgctt ttttagataa taatggactt
    68461 caaatagatg gagatataga aagcgtaatg agtttaggtt caatagttga taaatttaaa
    68521 gcttttggtt ggaatgtaat tgagatagat ggacatgatt ttgaccaaat atttgcggct
    68581 cttgatatag ctaaatcaac agttaaaaaa cctacaatga tagttgcaaa aactataaaa
    68641 ggcaaaggaa tatcttttat ggaaaatcaa gcaggatggc atgggacagc tccaagtgat
    68701 gaggagctag aaaaagcctt gcttgaatta ggaggtgctg acaatgagta aaatggcgac
    68761 gagagaagca tatggtaaag cattagtaaa attgggtcaa ataaatgaca atgttgtagt
    68821 attagatgca gatttatcaa agtctacaaa aacacatgat ttttacaagt catttccaga
    68881 tagatttttt aatatgggta tagctgaaca aaatttaata ggcgctgctt gtggattatc
    68941 aactgctggt aaaatacctt ttgcaagcac ttttgctatg tttgctacgg gaagggcttt
    69001 tgagattata agaaattcag tttgttatcc aaagctaaat gtaaaaatat gtgctactca
    69061 tgcaggtctt actgttggag aggatggagc atctcatgaa agtgtggaag atattgctat
    69121 aatgagagct ataccaaata tgacagttct tgttccagct gatggagttg aaacagaaaa
    69181 aattattttt gaaatagcta agtataatgg acctgtttat gttagattag ggagaagttc
    69241 agtaccagtt ttatttgatg aagattataa atttgaaata ggaaaaggta cagtcttaag
    69301 agaaggaaag gatgtatcaa ttatagcttg tggaattatg gtaaatgaag ctctattagc
    69361 acaagaaaag ttgcaagaag aaggtataag tgctagagtt ataaacatgt cctcgataaa
    69421 acctatagac aaagatttaa tattagaatc agcaaaagaa acgaatgcta ttgtaactgt
    69481 agaagaacat agcataattg ggggattagg ttctgctgta agtgaggtag taggagagtc
    69541 ttgtccaacc attgtaaaga aagttggaat taaagataca tttggagaat ctggtactcc
    69601 aaatgaattg ttaaaaaaat atgaattaac ttgtgatgac ataataaaga ctgttaaaga
    69661 agctattatt gcaaaaagaa tgtaagagat ataaattatt catgtttgtt aataatataa
    69721 tatatttaaa agagaggtat aaagtgttat attaatttat atctctcttt tttttatatg
    69781 atatcaatat atcaaaaatg taataaaata ttcaaatttt tacaaaaata gagaaaatat
    69841 tcaaatatat attgactatt cagaaaaata tgactaatat ataatttata gagaaactaa
    69901 ttttatatat aatgactact tagataaaag aatctaaaaa aattatgaga ggaggagtag
    69961 ggtataaaat cagattataa gttttaagtt attatactaa aaagctataa aacagttatc
    70021 atgtatttta tgttaaagga aattgtaggt agtttatcga attgtttaat cagaagaaaa
    70081 aatttcacat ggggaggtaa ctgagatatg tcagaaaaag taaatgttaa aacagtcatc
    70141 agtttcgcag gtgcgtatgt agcaacagta atcggttctg gatttgcaac aggacaggaa
    70201 ataatgcaat tcttctcttt ttatggtttt gcaggtatta ttggtgggat aatttcaatg
    70261 gtattattct catggatggg tgcttcagtt atgtcaaaag gtaaagagct tcaacttaaa
    70321 gaacctatca aaatttatcg tgtttattgt ggtaagtatt taggaatatt ctttgaatgg
    70381 tttggacctt tattcttatt tggtgttttc gtagtaatga tttcaggagc aggagcaact
    70441 ttaactgaat actatggatt aaatccattt gtaggtagag taggtatggc agtagtagca
    70501 ttattaactg tttcactagg tcttgataaa ttatcaaaga tactaggtgg aatagggcct
    70561 ataataataa tctttacact tttagttggt ggtataagtt tagctaaaaa cataggtaat
    70621 ataggagaag ctgcacaagt tcttagcact gtaaagatag ctaaaccagt tccaaatgcc
    70681 tatctatctg gtgtaatata tactacttat aacacaattg ttgttatggc attcttaact
    70741 ggattaggag caagtgctgc taacaaaaaa gaagctattt gtggtggtat attaggtgga
    70801 gtagcactta tggctgcagc tataatgatg cacttagcta ttctttctga tataggaaac
    70861 ttatattcaa aagcaatacc atcattattc ttagcagata agatttcacc agtaattgga
    70921 gttttattct caataattct tatattaggt atatatacaa cagctgtacc tttattatgg
    70981 tcagtaacta atagatttgt agatgacaaa catcctaagt ttaaattaat aactttaata
    71041 gcagctatat taggtcttat aggtggcttc ctaccatttg ataagctggt tggtatttta
    71101 tatccatata ctggatatat gggcgtcata atactggttt gtgttcttta tagacagatt
    71161 acaaaaacat ctggatatca agaaggaatc agtgacaaat aagtattttt taatattgta
    71221 aactcaagag ctatatgaaa acaatgaaaa tgttttcata tagctctttt tattttttag
    71281 ctcgtttatt atgaaataga aaaaacatgt tttttagtta cattacatat atttataatt
    71341 atgtataaat atatactaat caataattta gagtctaaga taagtttata aatcatatat
    71401 ttagaatata tgggaggagt gataagattg aatgtaaagt ttaatattaa aggtataatt
    71461 tatggagtta tagctcttgt attcgttata gggggattta tattcatacc aagtatgttt
    71521 gtagagaaat ctaaacccat agattataca gtattacaaa gaaattcaat accagaaaag
    71581 atattagatg tgatggataa gtatacaaat gaagaaagag cattagcggc taaaatcgat
    71641 aacaaaatat acattatagt gactagagga aataataaat atggtataga aatggacaaa
    71701 atagaaagtg tggttgagga aggaaaggat gttttgagag tcaagataaa gtataaagat
    71761 aaggaagatt cttttccata catagttgtt gaaacaaata tgagtgaact tccagataga
    71821 atagaattaa atgcgacgaa ataagaaggt attttataat taaataactg catattgtac
    71881 aataaagtca tggtttttat taatgtaagt aaattgtgta tggttcttat tgttgtaaaa
    71941 gccatgactt aaaatgctta taaagtaaga tattaacagt agtatttatt actacattaa
    72001 atggggtctg gaaatactta atattctaac tatttggagt gataattcta gtttcagtaa
    72061 ctatgtagtc taattgtgca tcatgagttt cttttggaat agaatcgaat atctggaagt
    72121 caaatgctat gccgatagtt atagcatctt ttcttaagtt ttctaaaaat ctatcataaa
    72181 atcctccacc atatcctaat ctatagcaat gtatatcata tgctacagct ggtacaatta
    72241 caacatctaa ggccttaata tcaattgtag gtgatttttc gttaggttct cttattccat
    72301 atgctccaat tctcaatccc tcttttaaat ctgttatttg agtagggatt aatgtatgat
    72361 tttcttttat tgtgatggga cttgatacta ttttctttag agacaaaagt tttttgatta
    72421 aattatctgt ttgaacttca ttgttaaaat ttagataaag cattatatta gttgcatttc
    72481 ttatatcttc catttgaagt aatttttcag ttattaaatt tgaattacta agtacaaagt
    72541 ttgcattctt attttttctt gattcaataa ctttttttct aaattctttt ttcataaaat
    72601 aattcctccc ttaatatgtt ataatgtgta cagcaattgt atttaatata aattttagta
    72661 taatttattg taataaagca atatatttgg gtaaatgtaa taaaaataac agctagtcat
    72721 ataataataa tagtttatgt aaaaggagat gtatcttgtg atttcaaaga agaaagctat
    72781 ttttttaggg gtgattttag taattataac agctatggtt acatcagcat ttcagttgac
    72841 attggggaat aaagttgtaa tatcgaaaga actctatgaa gactataaaa aatatgataa
    72901 gttgttagga ctagaatcta ttataaaaca agatttttat aaaaaagtat cagatactga
    72961 tttggtaaat ggagcagcaa aaggtctatt tctaggaaca aatgataagt attctggtta
    73021 ttatactaaa gatgaaatgg agaatctaat taatgatagt gaaggttctt atgttggtgt
    73081 aggtatgtat ataggggcat caaaagatgg tggcttagta gtagttccca tgaaagattc
    73141 tccagcagag aaagccggtg taaaatcagg ggataaatta gtaaaagtca atggaaaatc
    73201 tgtctcatat aaaaattcag atgaagctgt tcgtatgatg aagggtaaaa aaggtaaaac
    73261 agttgaactg actatattaa gagaagataa acaattgaac tttaaagtaa aaacagacca
    73321 gataattgag aagagtatag aaagtaaagt aattgataat gatttaggat atatagaaat
    73381 tacccaattt atatcaagta catatactga ctttgataaa gctttaaaag aattaaaagc
    73441 aaaaaatatc aagggattag taatagacct tagaaataat cctggtggaa tgctagatat
    73501 atgtaaagaa gttgctgatg agttaattgg tgaaggaact atagtatata caaaagataa
    73561 taagggaaat actgagtact tgaaatctga taaagaaaaa ttgggacttc caatagtagt
    73621 gttaactaat ggagaaagtg cttctgctgc tgaaatatta acagctgcaa tcgttgataa
    73681 taaagaaggt atttctgtag ggactacaac ttttggtaaa gggttggtac aatcagttgt
    73741 aagattgaag gatggaacag gatacaagct aactactgct caatatttta cacctaatgg
    73801 ggattatatc aatgaaaaag gtataaagcc tactattgaa gagaaagatg aaaataaaca
    73861 gttagatgta gcaactaagt ggcttagaga acagattgat aaataggatt taatttttat
    73921 aaacttatac tataaaataa agaacattat aaaagagatg tctcaaaatg aactttttag
    73981 ttcatgaggg agtctggaaa tatttatgtg tattctttaa ataaaaattt attttttaag
    74041 tgataattaa atttttacag acagggctat ggttttaaaa atcatagcct tattttgata
    74101 ttttttaaaa acatattgca acccagttgt tccaaatatt atatcattta cttttaaaat
    74161 atcgcaaact gcatattata tattcattat aatataattt atctaactag aacctcagtt
    74221 tactaattaa attagatgca tagaataact tttttaaaca ttgaaaataa attaaactct
    74281 aaacacattt aaatataaat ttatatatca ttttgacttg gttaaacaat ttcatactta
    74341 tcgatataaa aataggttat aatataaatg ttcattatct tataacctat tttatataat
    74401 ttattcctaa aacatgtgaa tgttttttat gatagtttga agaaattata aaatcacttt
    74461 ataattgaat attatatata aattaattta aatatagaaa ggaaaatata tgtacaacaa
    74521 tatcaaaaat attttaaaag aaaatcatat aacgtttgaa gaatatgacc atgaacctat
    74581 tttatcattt gaaaaagcta aagaaataag agaaaaatta caatatactg gagtagaaag
    74641 taaaagttta tttataaaag ataggtctgg aaattactat atatttgtaa ccacagaagg
    74701 aaaaaaatta gatccaaaaa ctataaaaaa tgttttaggt ggaaaagtat ctatatgttc
    74761 aaaagaagaa ttaacacaaa aaacacaatg tttgcctgga tgtgtatctc cttttggata
    74821 tgagaaaaat atctctcttg taatagatca agatatattt aaccatgata aaataatctt
    74881 ttcaccaggg ataccagaaa agacatttat tattacagct agtggattta aaaaagtaat
    74941 aaataatata aacaatacaa cttataaata tatatatatt aatgaatttt aatcataatt
    75001 gtaaattaga gaaaataaca ttagaaatca tgcttaacta tagtgtaaat aaattatatt
    75061 caacacaaaa gattgatact attattacaa tagttactgt taagctcaaa ttcaatgtat
    75121 tatgagttat tatttttaaa ttttaattac aaaagcaggg tagttttaac tactctgctt
    75181 tatatttaac ataaaatata taaaatcaac aaaatgattt tagtctatct attagatatt
    75241 cttcgctctc aatgcttaga acttttagat atatacaata agtgctttca tttgtaaaaa
    75301 tttctatagt attatcttgt ttatttacac ctacaatatc tttaaaattg atatgatttt
    75361 tagtattttt acatttgaaa atatatctgt tatcaattaa aatctcatca ttagtaattg
    75421 aaatataggg tttatctcat gctttattaa aaatttatat gtttgttcaa gataaaaact
    75481 tacaaataat aatagaaaag ttactttaaa tgtacatatt atcgaaaata ttatcacaaa
    75541 aatacttata atatatattt gtttaaaata acctctatcg caatagttta ttaacgatat
    75601 ggaaataaaa gtgttaaatc agattttgga gaaataactt taaatatttc cagactcttt
    75661 ttagacagcc tccataacct tatattgaac ctatttatta actttgtact tacatatacc
    75721 acttatataa gtttcgtata tattaaagtc cttatctgtt actataaaat ctgcctccat
    75781 accttgatgt atggagccac aaacattatc aatattacaa cttaaagcag ggatatatgt
    75841 agccattttt attgcatctt ctaaagtggc tatgttccaa tcaactatat ttctcacagc
    75901 cttgtccata gttaatacac ttccagctaa actcccacta tttaatcttg caacactatt
    75961 ttttacattt acattaaact taccaagagt atattttcca tcctccatag caccagctct
    76021 catacaatct gtaattaaag ctatgttatt actagactta gcatccatca ttattttaac
    76081 agctattggg cttacatgat gaccatcaca aattaattca gcataagtat cttttagtac
    76141 catagcagcc ccaaccattc caggttctct atgatttaaa ggactcatcc cattatatgt
    76201 atgtacaaac actttagcac ctgaattaac gacttgttta acttgctcaa atgtagctga
    76261 actatgacca agagaaactg atatattatt tttagttgca tatcttgtta attctattga
    76321 acctttacgt tctggagcaa ttgctatttt tcttattaaa tttttcgatt ttttttgcca
    76381 taattttaat atattgatgt cagggtcttt aaaatatttt tcattttgag cgcccttata
    76441 tttttctgta aaaaatggac cttctaaata aatcccttgt atttttgcac cctcaactcg
    76501 attgaagcta ttgcttatat tttctacaga atcacttagt gtatctacag aatcagttaa
    76561 agttgtaggt aggaaagatg ttactccata ttttaataag ccttttgaaa ttgtgtttat
    76621 tgcttcaaat gtattgtcca tgacatctgc acctaaaaga ccgtgaatgt gagtatcaac
    76681 taagccagga gcaatatatt tacctgaaaa atctataaca ttatcagtaa cattaggtct
    76741 ttcttgttga aattcaccga atttgccatt aattatacta aggtaacctt tttctttgat
    76801 tccatgtttc ataaaaaatt tatctgcata aataaaactt ttcaaaataa cctccttata
    76861 attatattat cattgtggca ttgacgctta taatatcatt ctgttttctt gaaagttatt
    76921 taacttacta tcttacatgt cttactctat atacaaattt atcacttcta gcgacactca
    76981 aagtaaattc aataactata ttgtcatcat tatatgtggt acgaagtaat ttcagacaag
    77041 gtgaatctcc tggtacttcc aataacttag cttccttatc cgagattatt ccagctgaaa
    77101 attcttcatc agcaacttta ataatctcgc cataatcttc tctaaatatt tcatataaag
    77161 gtttttgttc gattttttct tttgtcaatc ctataaattc tttcatagga agatatgttc
    77221 tttcaagcat cataggaagg tcatcagcaa gtcttaaacg cattattttt atcatttttt
    77281 caccaggagt tatacctaat ttttcagaga aatactttgt actttctact acctcaaatg
    77341 ataatacctt tgtacctggc ttttttcctt gtgttctcat ataatctgta aaactataac
    77401 tttccattaa gttttgagaa ttttgactta gtgcagcaac aaaagttcct tttccatgtc
    77461 ttttgtatat atatcccata tgctctaact cagccatagc tagtctaact gtggttctac
    77521 ttactccata ttcatcacaa atttttcttt cggaaagcat tctatcgttt ggttccatct
    77581 cattttcaat cttagttctt aatatctcaa caagctggtc atatattggt cttttatcat
    77641 ttctttttat cataaaaata tagtcctttc taagtggtta ttaccacaat ttgattatag
    77701 aatatagaat tatttatgtc aatgaataaa gtttaatcaa aattcaataa gtaaggctat
    77761 taatttatta taaaattatg gtacattgac aaaacaagta aaataattta tattcaaatt
    77821 ggtaataacc actttggata tattatatga ggtatttttt attttacaga ttaatttatt
    77881 gtttagaaaa gtaaaaatta ataatgagga gaatctagta tgattacagt aataacattc
    77941 aacccatcta tagatagaat gtatagagtg aataatataa acataggtga ggtgcaaagg
    78001 gtagtaagta agaatgcaac tgctggaggt aaaggattaa acgtatctaa ggtatgtaaa
    78061 attttaaaag aaaatccttt agctatggga tttctaggcg gatttaatgg agaatttata
    78121 aaaaatgaac ttagaaaact agatatacaa aataaattta caaaaataga gcaggaaact
    78181 agaaattgct taaacataat tgaggacaat aaagttagta cagaattttt agaaaaagga
    78241 ccaatagtag aaaaaagcga tttagagaga tttgaaaatg atatagagga agtactaaaa
    78301 aatactaaaa tactggtagc ttctggtagt tattgtgaga atatgccgat agactattat
    78361 gaaaagatag gaaatatatg tagagcaaat aatataaagt ttatattaga tacatcagga
    78421 gaagcattaa aaatagcttt aaaatcaaaa ccatatttaa taaagccaaa tatagatgaa
    78481 ataagacatt tattaggaat aaatatagaa tcaagagagg aaattatctt gtcaggaaaa
    78541 aaacttattg aaatgggtgc agagaatgta tgtatatctt taggaaaaga tggtatggtg
    78601 tatttaaacg aagataacgt atattatgta aaagtaccag ttgtaaagtg tataaatact
    78661 gtaggtagtg gagatagcac agtggctgga tttagtgtag gtattttaag gggttataaa
    78721 atagaaaatt tacttaaatt tgcaaatgct tgtggtataa gtaatgcttt aaatatggaa
    78781 actggatttg tagatttggg agaaattgat aaatatcaag atttagttga agtaaaaaag
    78841 ttaagttaaa tacatttaat gggaatataa taaatttaat gttgttgtaa tagattttaa
    78901 ttttaatata aaagatatag tcttatataa aaataggtta gaaaaattca tttttaacct
    78961 atttttataa taaatatcta ttttactgaa tttataattt tctctagtaa tctttgagcc
    79021 ttgatacact cttctccact gatagctggt tttatattat tttctataca atcaataaaa
    79081 tgatttgata tatcctcaaa acccttttgt tttaatatat ttacccatgc accactttgc
    79141 tttaaagtta ggtttccacc ttcttctatt tccagtacat taagattctt aactctttga
    79201 atcttaccct ttgaaagaat ttcaacttgt tctaactttg ttccagaatc tctatgcata
    79261 gaggtgttta tagagaaatt tttaccttta agtttatgac ttacaaatat aagattctta
    79321 ttgtctgtta aaaataaatc ctctccacta atttcaacat cttcatttgc taaccataaa
    79381 gcagtgtcaa taacatgtat atagtcatct ataagagtac tgtcgaattt aacatttctt
    79441 aaggaattta atccatgttt acatatattg atagatacaa tttctgtagc attattttta
    79501 atttctttgt acataggaca gaatctacgg ttgaatccaa ccattaaatt taagtttttt
    79561 ttcgtactta attcgataag ttcttctcct tgacttactg ttgaagcaag aggtttgtct
    79621 acatatacat gaacacctaa atttaataat atttttataa tttcgtagtg tgtttctgta
    79681 gagctatgta gaaaaataca atcacatttt ttagctaggg attcaataga atcgaattgc
    79741 atgattctat aatcactaca gactttttcc cttttaactt tatttggagt aaaagctcct
    79801 acaaactcaa atctctcaga cttagttaat ataggtaaat atgccttttg agctatggag
    79861 ccaagaccta tcattcccat ttttatattt ttcataatac tttctcctaa ggtttttata
    79921 tttacagaat tgattataat tcagacacat aaatttgtaa actttgtatc aaaaaataaa
    79981 tttaaagctt atataagtaa attagcgaaa taagaaatat tgataaagta attttgtagg
    80041 atatctataa aattgattga tataaatatt ttatgtattg acaaaaaatt caaattaatt
    80101 tatatttgga ttagtggtaa ttaccacttt ggtctgaaca agtttttatc gtaaaataaa
    80161 aatactaaag tatatagagt atgattttat taaattttta aagataaact ataaaattaa
    80221 ttaaaatcta tagtgtatat tttatagtga gtttaagtag tactaaaaat ctaagcaaaa
    80281 aattataaaa gagtatattt ttttatagtt taaagtggtt ataacctcat taccaaatgt
    80341 aaacgttaca agtatgaaag gagaatgaaa atgtttaaat tagaagaaca aaaactaaaa
    80401 gatttaggtg caataataac aactaatgaa ataaaacaac agcctgaatt atggttagag
    80461 acttatgaaa tatataaatc taacaaagaa aaattaagta gattcataga tacaataagt
    80521 aataatcatg ggcagtttag agttatattt acaggtgctg gaacttcagc ttacatagga
    80581 aattcaatac ttccttacct taaaaataaa aatgacataa gaaaatacat atttgaagca
    80641 atacctacta cggatatagt ttcaaaccca tatgattact taaaaaaaga tataccaaca
    80701 ttattaattt catttgcaag aagtggaaat agtcctgaaa gtttggctgc tttaaattta
    80761 gggaataaga ttgtagataa tttttatcat ttagctataa cttgtaatcc agaaggagaa
    80821 ctagctaaga tgactaaaaa cgacgaaaat aattacttgt tattaatgcc atcaaaatca
    80881 aatgatgaag gctttgccat gacaggaagt ttttcgtgta tgatgttatc tgctatgtta
    80941 atattcgata gtttagaaga tgatgtagaa aagtcatata taaatgcaat agttgaaatg
    81001 ggaagaaatg ttatagatag aaaagatgaa atacatgagt taataaataa agattttgat
    81061 agagttgtat atttaggttc aggaggatta ggtggtttga cacaagaagc acaactaaaa
    81121 cttttagaac ttacagcagg aaaaatatct acagtgtatg attctcctat ggggtttaga
    81181 cacggaccaa aatcatttat tgatgaaaat acacttgtat ttgaatttgt atctaactgt
    81241 ttatatacaa gaaaatatga tttagatgtt ttagaggaga taaaaaggga taaaattgct
    81301 aaattcactt gtgcagtgtc agtagaaaat gaaaataact tctcaggaac aaagtttgag
    81361 tttaaagaaa agtataataa attaccagat gtgtatcttg caatgccata catattgttt
    81421 gcacaaacta tagcattgtt tgtatcggta aaggttggaa ataagccaga tacgccatca
    81481 gctacaggaa cagtaaatcg agtagtaaaa ggtgtaacaa tatatgaata ttaaaagata
    81541 agattaaaat attataacta aataatttgg gagggtatca atgtggcatt agtaacaaca
    81601 acatcaatgc ttaaaaaggc acaagatgaa gggtatgcag taggagcatt taatgtagaa
    81661 aatatggaaa tggtaattgc tgttatagaa gctgctgagg agttaaattc tccagtaata
    81721 ttacaaacta ctccatctac tgtaaaatat gcaggtcttg attattatct agcaaatgca
    81781 aaggttgctg cagaaagagt aaagatacct gtagcaatgc atctggacca tggttcaagt
    81841 tttgaccttg caatgaaagc tttaagaagt ggatatacat caataatgat agatggttct
    81901 catgaaagct ttgaaaataa tatatctata tctaaatcag tagtaaaagc atgtagtcct
    81961 tctcttatac cagtggaagc agaacttggg aaagttggtg gaaaagaaga tgatttagat
    82021 ggcgggaata gtaatgatta tacaaatcca aaagaggcaa aagagtttgt cgatagaaca
    82081 ggaatatctt cattggctat tgctataggt actgcacatg gattgtataa gggtgaacct
    82141 aaaatagatt tagataggtt atcagaaatt aaaaatgtag tttcaatacc tttagtattg
    82201 catggaggtt caggaataca tgatgatatt ataaaagaag ctataaaaag aggtattgca
    82261 aaagttaact atgcaactga attaagaatt gcatatagta aaggggttaa aaaagtttta
    82321 gatgaagatt ctgaggttat agaccctaaa aagtatggat tagcagggct tcaatcagta
    82381 aaggattttg tgaaagaaaa gattaaagtg tgtgggtctg taaatagagt ataaaaataa
    82441 aatatcctaa atttatttta gataagttta ggatatttgt tatatgaaaa atttatatta
    82501 attatatgta aatggtgtat ttttttcatt taattgatga aaaatatcag cttttattgg
    82561 taaagtatta catatataat taaaatatac taaaaattga actatatatt ttattattat
    82621 aaaatattta tattaaattg tggaattaaa atatactagg atataaaatt cttaaataaa
    82681 aagaaaagat tgtttttata aaaaatattt tttataccac ttaaaaaaac acaataataa
    82741 cactgaggga ggtggggata tgagagaacg cattattctt gaaacattaa aaaatagtag
    82801 tggttatcta aacatagatt attttgcaga taagttgggg gtatctactc gaactatcag
    82861 aaatgaaata aaaaagatag aaactataaa agaatgtaat ggttttaaat tagaatataa
    82921 gtctaaatta ggatatattc taaatattaa agaccaagat aaattcgagc attatctaag
    82981 aaacttaccc tcagatttag ttgaaaatcc agaacaacgg ttagagtcta taatagtaga
    83041 acttttagta aatgaaggtt ataaaactat agaacagtta agtaaaaaat ttctagtaag
    83101 ttcatcccaa ataaaaaatg atttaaaaaa gatagatgaa aagataaagg atacagaatt
    83161 aaaactagag cgaaaggctc attatggaat taaaatagaa ggttctataa aagaaattca
    83221 aagtatattg gtggatagtt attttagagg gaatagaaat ataacagagt acaggaataa
    83281 gtttatagat aatttaaaat tagctaatat tagaagtaca ataaaaaatg tacttaatga
    83341 acatgattta gaagcaaatt taacagaact tgaggaaata ttagcacaaa ttattatatt
    83401 gtatataaga gttagtatga gagtagcggg aaatgctagc gatttaaaat tatatacaga
    83461 agatttaatt atagataaat tattagataa aatttttaga gataaaaaat ataaccttaa
    83521 ttatgatgaa aagtattatt taaaggagct tataaaacta aagactaaag ataagaaagc
    83581 cactataaag aatattgata agaataaact tcaagatatt atgttcgaat tttttagaga
    83641 gatagataaa aaatataact ctaatttttt agaagataaa gagttcttta acttatttta
    83701 cttacatatt gcatgtttaa tagaacgtat aaagaagaac cacaagataa taaatccttt
    83761 ttcagttaaa ataagtcaac agtatccaac tgtttttaat ttatctattc aattttcaaa
    83821 aatcatagaa aatgagtatc atataaaaat aagtcaagat gaaatagggt ttatagctac
    83881 tcacattgct gttccttttg aaaagagaga agaagctaat tttaataaaa aatataaaat
    83941 agcaataata tgctcttctg gtggaggtag tgcatttttg ataaatttaa gacttaaaga
    84001 aatttttcca aatgctgaga taagaaattt ttcattacta gaagaaaaag ctgtaataga
    84061 atttgcacca gatttgattt tttcaataac aaatctttta tttgaaataa atgctccagt
    84121 tatacttata aatgaaattt tagacgaatt ggattattta gatattaaag aaagtgttag
    84181 atttgcagac catataggaa atatatcaag cccaaaacaa tatatattag gtctatttga
    84241 taagaatcac tttaggtgta taaaagataa agctaagtat aaggatatcc ttgatagtat
    84301 gtcaaaatgt atagttgacg agggcgcttg ttctccaacc tatcccaaag atgtatggga
    84361 aagagaaagc tatttgagta ctatttatac aaatggggtg gctatacctc atccaatcga
    84421 gatgacagga aacaaaaata taatatcagt agctttgata caaactgata ttatttatga
    84481 aaatagggtg cctaaaatta ttttcatgat ttcattaata aagggaaatt tagaacttca
    84541 taagcaaata tctaaatatt taagtaagat tatggcaaat aaggatatgg tagacatgct
    84601 aaataagtca caatcttatg aagagtttat gtataagtta aaaatctatc tgggaggtta
    84661 gacatgaata tagagttttt taagaggttg tcagaagcag atggtatagc atctaatgaa
    84721 gaagaggtta gacatgtttt attggaagaa ctaaaagctt atagtgataa aattatctgt
    84781 gatgggctag ggagtatcat cttttctaaa ataaaagatg agagtgcacc aaacgtaatg
    84841 atttgtgctc atatggatga agttggattt atggtgagaa gtatagacaa acttggtatg
    84901 attcacttaa taacaatagg aggagtaaaa cctcttgcac agtttgtaca aaaagttaga
    84961 attacaacaa aagaagggaa gaaaatccct gctgtcataa atgctacata taataatggt
    85021 aaggctgaaa atatatatgc agatattgga gcatatacag aagaagatgt atataaccta
    85081 gggattaatg ttggggatat ggtaacatat acaacaagct ttgaggaatt tactttacca
    85141 gaccgattag tgggaaaggc ttttgatgat aggataggat gttttgtgat gggagaagtt
    85201 ttaaaagaac ttaggaaaga aaatctaaat tgtaatattc actttgcggc tacaagtagt
    85261 gaagaggttg gaataagggg agcaaagaca tcaacacagt taataaatcc agatatagta
    85321 tttgtaatag atgtagcatg tgctaaaaat gagtttgtga gagactatac aaatcagaaa
    85381 caaattgata aaggtgtaat gctaatgcat agagatagga ctctagtccc tagtagaaaa
    85441 atgatagatt atgtaatgga aattgctgat aaaaataata taccacttca acatgatatg
    85501 tttgaatcag gtgggactga tggaggagaa gcacatctcg taaatgaagg gaaaccttgt
    85561 gtagtaactt gtgtaccagt tagatatggt cattgtgcat tttcaatagt aagtaataaa
    85621 gatttagaga acattataag attgtataca cagttaattt taaattttga tgaagaacaa
    85681 tataaacata taagaaattt tatctaaagg agaataagaa aatgtcaaca gaagtacaaa
    85741 taatggaact tatatcaact gcaggagaga gtaaatcgaa agcttttgag gctttaaaaa
    85801 aagtgaagtc aaaagatttt aaaggagcaa aattgttaat agaggaggct cgtaaaatag
    85861 atattgaagc tcacaggata caaacagaat taataactca agatttaaat gaagatgttg
    85921 aaagtccagc aatgagtctt ttaatggtac atgctcaaga ccactatatg acttctcagc
    85981 tagcaaaaga cttgatagag gctttgatat attgtcttga taagtaattg taatatagaa
    86041 atagattatt tatgtaaaaa ttcttttaaa agtttagatg cttttaagag ataataaaaa
    86101 tacaaacttt aggaggaatt attatgaaaa tattattatg ttgtgcaggt ggattttcta
    86161 ctaatatgtt aatgcaaaat atgaaaaagg tggtaaaaga gagtgctaaa ctcaatgaaa
    86221 aagattttga gtttacagct ataccagcag attcaataga aagtgaagtt gatgattggg
    86281 atatagtact tataggtcct caaatagctc acaaaataga cttcttaaaa ggaatattag
    86341 agccaaaaaa taagcctttt gcagtaatag ataaagatgt atatggaaaa atggatggag
    86401 ctacagttat gaagttggca ttaataactt ataagaaaca tcaattagct caaggataat
    86461 aaaaataatg agggaaaagg gagattagat atgttttcaa aatttgaagc atttatgaat
    86521 agagtattta tgcctttagc tcataaggtt gacaatcaac gtcaccttgg ttctataaag
    86581 gctggtatgg ttgctatgac accattcaca atattaggaa gtatatttgc aatacttcca
    86641 gcacttccaa atatgcttgg agaaaataat ccaataagca aattcatact taacaattca
    86701 gcaatattgg atttaccagt aagtctttcc ataggtttaa taggattata tgcttgtatg
    86761 tgtattgctt ttaatctggg gaactactat aaattatata taccaggttg tgttgcacta
    86821 tcaacttttt cttttttatt cctagtagca tcatttacac cagatggagc tttagaaata
    86881 ggaaatttag gagctaaagg gctatttaca ggtatgatag cagggcttgg ttctgtggag
    86941 ttatataatt tttgtaataa gaagaatatc acaataaaaa tgccagaaaa tgtacctgat
    87001 tttgtatcta ggtcttttga attaatacct actactataa tagtttgtgg aacttttata
    87061 gttgctcgtt ttatttcatt aaaagtattt ggagaattac ctccacagat acttactcaa
    87121 tttttatctc cattagtagg aagtatggat aacccatggt cagttcttgc acttaacttt
    87181 gctatatgta ctatattctt ttttggaata cattcatcag tatttagtcc tataactaga
    87241 cctataatga ttacatttat agctgaaaac atagcagcta tgcaagctgg agaaacaata
    87301 cctcatttct ttacagcagg agcttcaagt gcattctttg gatttacagg atgtggaatt
    87361 agtataggtt gtgtaattgc atgtatgtta tcaaaaaatg cttcctataa gaagattgga
    87421 cgtgtatcat tattccctgc attatttggg ataaatgaac caatattatt tggagcaccg
    87481 ataatattaa atccaataat gtttatacca catgtatttg gtggagctat aataggtaca
    87541 ctaccaatgt tttttatgca ctggggatgg ttagcaaagc cagtatttga ccctccatat
    87601 gtaggggtat tcttagaagg atttttaact aatggagatt ggcattctat attagcaaat
    87661 gcattgcaat tagtattagc agttttatta tattggccat tctttaaaat aatggaacgt
    87721 caacaaaata ataaaaaaca aaaagaagaa actaagaata tattctctaa agaagaagaa
    87781 gacttattgg atgatttagg attggatttc taatattttg ttatgaataa tacaatggag
    87841 agattagtat ccttaatcta taaaagaggt gttgacgctc ttttgattaa agcaaaaaat
    87901 actaaaagat atataggagc tttatcaggt agtggtgtat atgtgcttgt tactaaggat
    87961 aaaaattatc aaattctaga tggcagatat attgatgaag cagataaaaa aacgactggt
    88021 ttcataaaaa ggattgtatc tcaaggttct tatataccaa ctataattga gttattaaaa
    88081 gaattaaata taaaaaagtt agcaatagaa aaccaagcta tgagtataca agagtatctt
    88141 tcactaaaaa atgaaggttt tgaaatcaat ttaataacaa atgaattatg gaagctaagg
    88201 gctataaaat ctaaagaaga aattgaactt attaaaaagg cttgtgaaat tacagatgaa
    88261 gtatttgatg aggttatttc tgaaattaaa aaagatatga ctgagctaga agtatcagct
    88321 ctaattcagt atcatgcatt aaaaaaaggt gcaagtggaa tgtcttttga aacaatagtg
    88381 gtatcaggag aaagaggtgc aatgccacat ggaagaccta caaacaaaaa attgaaagtg
    88441 aatgaagcta taacaataga ttttggtgtt gtttatcaag ggtatcaatc agacatgact
    88501 cgtacaatat ctataggtaa acctcctaaa attattaaag agatatatga tgtagtattg
    88561 gaagcacaat tatctgcaat agagtcaata aaggaaggaa ctagggcaag tgatgttgac
    88621 aaagtggcta gaaaaattat agataaacat ggatttggcg aatattttaa tcatggactg
    88681 ggtcatggta taggtcttgg agatggtgaa gtccccactt taaatccaaa tagtgaagat
    88741 atattagtgg aaggaatggt aatgtcttgt gagccaggta tttatattcc aaatgtaggg
    88801 ggagttagaa tagaagatga cattgttata atagatggaa aaggtgttcc actcaataag
    88861 acatccaaag agtttataat attaggagag taaaattatg aaatataatt ttaataaagt
    88921 attaaatcgt aaagaaggaa attgtagaaa atggagcaac catgtgatta aagaaaaatt
    88981 cggcttaaat gaagatgcaa ttcctatgga tttagctgat atcgattttg agtgtgctcc
    89041 tgctataaaa gaatctatct taaaaagagc atcagtagga gattatagtt atacttttat
    89101 aggagatgac ttctatgatg cgattataaa ttggaataaa agaagatttg atgtatatat
    89161 agaaaaggat tggataaaat taacttttgg tactgtttca acccttcatt atatagtcca
    89221 agcatatact aaagaaggag agggtgtatt gataaataca cctgcttatg acccttttgc
    89281 agaggctgta gtaaataata atagaaagtt gtgttgtagc tcattaaaat tagataacaa
    89341 tagatattat ttagatttag aggatatgga aaatcaaatg aaatacgaga atataagatt
    89401 gcttatattc tgtagtccac aaaatccatc aggtcgtata tggacaaaag aagaactata
    89461 tcaagtgtca gagttatgtt taaagtataa tgtaattttg gtaagtgatg aaattcatag
    89521 agatataata tttaaagatt atagatttgt atctttatgg aatgctcatc cagaaattta
    89581 taaaaatagt ataatctgtg tatctcctaa taaaggcttc aacttaggtg gattaaaaac
    89641 atcatatgta ttaataagag atgtaaaaat tagggagact ttattagaaa gactaaaaag
    89701 taattcaata acttctccaa atgtatttgc aataccagca atagttgcag catataatga
    89761 aagcgaagaa tggctagatg ctatgactag ttatgtagag gagaattttg aaatagttta
    89821 tgatttcttt gaaactaata taccaaaagc taaagtgatg aagtcagact cttctttttt
    89881 agcctggata gatgtaagag aagtatttag aaatgaagaa gaatctaaag aattttttag
    89941 acatgcaaac ttaactatgg tagttggaag ttattttgtg aaagatggag atggatttat
    90001 tcgtattaat gtggggtgtc ctagagaaac attaaatgag gctctaaata gaatcaaaaa
    90061 aacgtatata agtatgtatt gttaaatcat aaaaaaagta tctatggttt atataaaaac
    90121 acattattta gttatcaaat attactggaa ttttgtattc cagtaatatt ttttgattaa
    90181 ggaatattat tgaggaatat taaataatat tatttcatac ccttatatta acttatataa
    90241 aaatatttta ttattttggg aacgggttag ctaaccttta tctagtgtat atttatattg
    90301 taagaagcag taaaaacttt tcctttacta agtttattaa taaacttctt atctttataa
    90361 attttgttag aatttatact atcagcaaga ccataccaag gttctataca taaaaaagga
    90421 gcagtagaat tagtttcact ataataagga gtccaaatac caactagagg gaaatcttcc
    90481 atagatatag ttacatattt actaccatca ttattacata tagatacttc atctatgttg
    90541 gtgtatatca aagcatcatt cttgaatact tctggattta actgtaagtt ttttaatttt
    90601 ccaataggta ttatatcatc tgtaaaagaa ccatttaaat tgattttttc tacatcattt
    90661 ttacttttaa actcaaggta gtgtttagaa aaatcatttt gttcgtaaaa aggaaggttg
    90721 aatgcaggat gtccacctat agaaaagaat atatctttag aatcagtatt ttttacaatc
    90781 cattctacct caatactatt atcatttaat gtatagttta tgattagctc aaaagaatat
    90841 ggatacttct ttaaagtaga ttcattatct gttaatttat aagttacaaa tgtatctcct
    90901 ttatctacaa tctcaaagtc catatctcta gcaaacccat gttgagtcat gctatataaa
    90961 ttttcttcta ttattgtttc attatcaaaa agtttaccta ctatagggaa caatataggt
    91021 gattgtctac cccaatattt tttattacca tcccaaagaa tgtttttgtc gtatttttta
    91081 gaaaatattt tagttagttc agcaccaaaa ctgcttgatt cgataattaa attttcattt
    91141 tgtaatatat tcatttaaaa tctcctttat tatattttat gagtatatta tactataaag
    91201 tataaagatt attaaatttt atttgatatt atatttgaaa ggtagatttt agtatatttt
    91261 gaaaattttg ttttgaaaat ttgggattat ttaagaaatt ttgtattata aatagaagga
    91321 aatagataaa ttgtatagaa taaaatatat taagataagt taataatgtt aaatgggaag
    91381 tgagtttatg gaaaaaccaa atacaaaagt tgttaacttt caagagtata aagacagaaa
    91441 aaaccaaaga gaagtaaata gagacgaact tttaaagctt ataaaaaaaa ttgtgacttc
    91501 aaaataggga gttgttaaac tccctatttt aatgggtttc ataataatta agacattctg
    91561 agtccatcta tataatcttt gaattgtgag aaatctgatt cactacttaa ttctatggtt
    91621 tctgaacctt ccgtataaaa attcttaaga tagatagtat aattattatc aacacaatca
    91681 tgataaatac atctatatcc atcttcatac aaacttttta acttagaaaa atcatccaca
    91741 atatcatctc cttaaagttc ttttaaatat atttttacaa aaagcatcta attgtatgca
    91801 aattgacaat gtaaaataaa ggaaatgaac ataaaatgta gaataatttt tatatgtatg
    91861 taaaaataaa aagaaaaagt tgaaattttt agaaaataag agtatttttt atctaaaaag
    91921 agagtttaaa aaataaaaaa atgttgggag gataaaaaat gagtttatta gaaagtatat
    91981 caaaaaacat atattcccta gataactctt ctattgaaaa aacaaagcag agattggata
    92041 ggcttataca tccaactgga agcttaggaa agatagaaga tatttgcatg caattagcag
    92101 gaatatttgg caatgaaaat tttgacacaa gtaaaaaggt tataattgct tttgcgggag
    92161 accatggtgt atatgaagaa ggcgtagcac ctgaccctca aaatataaca aaattacagt
    92221 ttccaaattt ttcgaaaggt ctatgtggag ttggagtaat aagtaaattt gtaggggcag
    92281 atgttgtagc tgtagatgta ggaataaact gtgatgaaaa actggatggg gtattagact
    92341 acaagataag aaaagggaca tcaaatatgg caaaaggtcc agctatgagt aaacaagaag
    92401 caattagatg tttagagata ggtattgaaa tagcagagca gtgtatagaa aaagattata
    92461 aggttattgg tattggggag atgggtatag caaatactac tccaagtaca gcaataatat
    92521 ctgtaatttc agggtgtgac ccgttagaag ttactggtat aggtgcaggt cttaagaaag
    92581 aaagactaaa gcataaggct gaagtaatta gaaaggctat tgaaattaat aatccaaatc
    92641 caactgatgg agtagatatt ttatctaaag taggagggtt tgaaataggc tcaatggcag
    92701 gtgttatatt aggttgtagt gctaatagga ttccagtagt aattgatggg tttatttctt
    92761 atgcagcagc attaattgca tataagataa atccaaaaac tagagaatat atgatagcat
    92821 ctcatttatc agcagaatca ggaactaaaa gagcattaga catattaaaa ttagaccctc
    92881 tattaaatat ggatatgaga ttaggtgagg gaagtggagc tgctttagca tttgatataa
    92941 tagaagcttc aaactatact tataaaaata tggcaacttt tgatgaaatt gacatgggaa
    93001 gataaatata aaaatttaat atgtaggagt gttttgatga gtaaaattat tctagtaaca
    93061 ggaggagcta ggtcagggaa gagtaacttt gcagaaaaac tatgttttga cagaaataat
    93121 aatacagctt atatagcaac ctctattcca tttgatgatg aaatgaaaga tagagtaaaa
    93181 aaacatcaag agaatagacc tagaaactgg aatacttatg aaatatatga agatatatat
    93241 tccataatta aggagatata taaagaacat gaaactgtta tactagattg tgtaacatta
    93301 ttagtaaata atctaatgtt tgaacacaat ctaaatatag aagaatctag tcaagaagaa
    93361 ataaacaaat tagaacaata cataaaagaa caagttgtca aacttattga agaaatagaa
    93421 aaaacaaatc tatactttgt atttgtaacg aatgagttag gtatgggaat tgttcctggg
    93481 aataaactta gtagaattta tgctgatatt gtaggtcgta taaatcaata tatagcttcg
    93541 aaaagtaatg aagtttattt tgtagttagt ggcatagcta tgaagataaa ggagtagtat
    93601 gcaattatga aaagatttat tttaatacta cagtttttga caaggatacc tataaaatta
    93661 aatgtaggat ttgatgatga attttataag tcaatagtat attttccact tgtaggtttt
    93721 gtcataggta tattatccta tttaatagga tggatttcta tgcttttatt tgaaccattt
    93781 atagcttcta taataataac tctagctggt gtcttaatta ctggaggact tcatatagat
    93841 ggattaggag atacatttga tgcaatatat agttataggg ataaagaaaa gatgcttgaa
    93901 ataatgaaag attcaagatt aggtacaaat tcacttctag caattatgtt tgtactttta
    93961 ttaaaggttg gattcgttta cgatataata agtaataatt cattgtgggt tataattttt
    94021 atgccaatga ttgcaaggtt aggagtaatg ttattgacat ataaaactgt gacaccaaga
    94081 gaaaaaggaa tgggaaattt atttatagga aaattaacta caagtatgtt aataactgct
    94141 ataatataca cattattgat agttgctctt attactaaat ttatattttt attacctaat
    94201 atagtgttga ttaaagtatt aggttcgatt atagtggttt ttgtatttat aatattattc
    94261 aaaaaacata tttataaaaa aattgacgga gtaacaggag atatattagg ctgtggaata
    94321 gaactttcag agcttgtata tttaatttac atatatttgt taatttttat gtttttttaa
    94381 agtaaaaagt ggtgataata tgataagatt aatattaata agacatgcac tgacaaatga
    94441 taataaaaaa ggaagattat caggccatat aaatagttgt attagtgaag agggcaaact
    94501 acaaataaat aaaattacca gatatctgag taatgaaaac atagataagg tttatacaac
    94561 accatctaca cgtactaaag atacagtaga aaaaatatca aaattgaaat tattagaaat
    94621 agaagaaaaa gaagctttaa gagaaattag ttttggagac tttgaaggaa gaacttttga
    94681 agaaattaaa atcaaatatc caaatgagtt tgaaaaaatg ataaaagaag gtaacaacta
    94741 tagatatcca aatggagaaa gtttgataga ctcttataaa agagtggcaa aagaaattga
    94801 taatataatt ttagaaaatg atagtaattt agatacaaag acaatactta tatgttctca
    94861 tgcaggaaca ataagaaata taataacaca tttgatttct ggtagttata agtatcactg
    94921 gaactttaaa attgataatg catctattac agttttagaa attgatggtg gatttgctgt
    94981 aatagataaa atgaatttta cagacttcat ataatttaat caaaaccaaa cattacaatc
    95041 tgtgtttgta atatatctgt aaaattagca atgaaaatag aatattagat taaaattagg
    95101 ttcttaatta gatgttaaaa gggaaaaagg ttagatgcct ttgcagcccc cgctactgtg
    95161 aaaccaacga aactataaat accactgtca gtttgatggg aaggttatag aagtaggatg
    95221 aagttaagcc aggatacctg cctaatttaa tttaaaacaa gaggtcttcg gggtgaaaga
    95281 tttttaatta atatatataa aagtcctatc tttagaagta ttaggacttt ttttaatttg
    95341 ttttttagta aattttaaca aggagggatg gaaagtgggg aaaaaaataa tgctacaagg
    95401 aacagcttca aatgtaggta aaagtgtctt agtagcaggt ttgtgtagaa tatttaagca
    95461 agatggttat actgttgctc cttttaagtc acaaaatatg gctttaaatt cttttataac
    95521 taaagaagga ttagaaatgg gaagagcaca agtatttcaa gcagaggctg cgaaaataga
    95581 gccaatagct gatatgaatc cagttttatt aaaaccttct ggaaataata aaagtcaagt
    95641 tattgtaaga ggtaaagttg taggagaaat gccttcttct aagtatcatg attataaatt
    95701 ggagttagta gatgtgttga aggaaacatt taatagctta aattcaaggt atgatattgt
    95761 agttatggaa ggggcaggaa gtaatgcaga gataaactta aaagatagag atatctctaa
    95821 tatgggtatg gcagaaatag ctgatgcacc tgtaataata gtcggagata ttgatagagg
    95881 aggcgtattt gcctcattag caggaacaat gcttttatta aatgaagatg agaaaagaag
    95941 agtaaaaggt gtaataatca ataaattcag aggaaaaaaa gagcttttac aagatggtgt
    96001 aaagatgcta gaagacatca taaaagtacc tgttatagga gtagtacctt atacagatat
    96061 aaagatagaa gatgaagaca gtgtaactac gagatttaag aaaaaaatga ataaaagtga
    96121 tatacacata gaaataatta gaactccaca tatgtcaaat tttacagatt ttaatatatt
    96181 tgagacacaa gaagatgtaa gtattagata tgtagactat ggagaatctt taggaaatcc
    96241 agatatatta ataatacctg gaactaaaag tactatagac gatttaacat atataagaaa
    96301 aagtggtctt gaatcgcaaa tcaaaaattt acatagtcaa ggtaaattga tatttgggat
    96361 atgtggtgga taccaaatgt taggtaaaaa attaaaagac ccttatcatg ttgaaagtga
    96421 aattgaagaa gttgatggaa taggtctttt agatacagaa acaacgtttg agaaagaaaa
    96481 gactacaaca caagtagaag ctactattgt tgaatgtatt agtgaataca tgaataacct
    96541 taagggaaaa aaagtaaaag gttatgaaat tcatatggga attacaaagc gaggtaatga
    96601 tgtaagaagt ttagatttaa taactaaaaa acttgataaa gaagtgaatt atactgaggg
    96661 aagtgtaaat aaagagggga atgtaattgg gacttatctt catggaatat ttgatgaaat
    96721 aagctttaca agagcaattt taaacaatat aagaaagaaa aaaggtttag gagaattaga
    96781 aagcaaagta aactctttta atgaatttaa ggataaagag tatgataagt tggctgattt
    96841 tttaagagaa catttagata tagagaaaat atatgagatt atgaaataat gatgaccttt
    96901 atttataatt taggtaggag gtgtattatg aaaaaaattt taattgcggg tactaatagt
    96961 ggagtaggta aaacaacaat atcattagga ataatgcaag ctttggttaa gagaaatatg
    97021 aaagtacagc catataaagt agggccagac tatatagacc cttcatatca cacttttata
    97081 actggaagac attctagaaa tttagattct tatatgttag atgatgaaaa aataaagtat
    97141 atagtaaata agtcctcaaa agatgcagat atttcagtaa tagaaggtgt tatgggctta
    97201 tatgatggct ttggaataga tttagataat tgtacaagtt cacatacttc aaaggtatta
    97261 aagtcacctg ttatattagt tataaatggt aaatctatgg cagcatctag tgctgctatg
    97321 gtacttggat ataaggaact agataaagat gtaaatataa agggtgttat agtaaataat
    97381 gtaaagacta atagccatta tcaaataatt aagagttcta tagaaaaata ttgtaatgtt
    97441 gaagtattgg ggtattttcc tccaaataac aaattctctt tggaatctcg acatctagga
    97501 ttagttccta gtgtagaaat gaaatcacta agggaaaaat tttatacctt agcagatgaa
    97561 attgaacact atatagatat tgacagaata attgaaatat cagaaagcga tgaatttgag
    97621 agtgattttg attttcaaac tttaatagaa aataataaaa taaaagaaca tgttaataat
    97681 aaatcaatag ctatagcata tgataaagca ttcaattttt attatagaga aaatatagag
    97741 cttttagagg aacttggttt agaaataaac tattttagcc ctcttgaaga tgtatcagtg
    97801 ccaaatgcag attatattta tattggagga ggttttccag aaatatttgg aaaagaatta
    97861 gaagcaaatg agtcaatgag aacttccata ttagaagcac ataacaataa tatacctatt
    97921 tatgcagaat gtggaggact gatgtaccta ggtgagaaac tattgaatca agaagaacaa
    97981 gaatttaata tggttggaat atttagtgga attagtaaga tgacatcttc tttaaaaaga
    98041 tttgggtact gtattggtgt agccaaggaa aatactcttt tatctaaaaa aggggaatct
    98101 attaaagggc atgagtttca tcattcaata tttgaaagtg atgaaaaatg tgtatattca
    98161 atgagaaaag aaagagatgg aaatatagta gaagagtggg atggtggtta tagtaaagga
    98221 aacactttgg ctacatatct tcatactcat ttttataata acttaaattg tatagaaaat
    98281 tttttgaaaa aaggaaatga ataaatgagt attttatcta tttatatagg atatgtgaca
    98341 gacttaattg taggagaccc atattctttt cctcatccag tgagatttat tggtaaactt
    98401 ataaatttta ctcaaggtat aataagaaag gtctctaaaa atgataggca gttaaaattg
    98461 gggggattta tattatggtt tataaccgtt ggaataacat atttaacaac ttatgctata
    98521 gtgaagttat ttagttttaa tatattattg agtattgtag tgaatagttt tataatttat
    98581 acaacactag ccacaaaatg tttaaaagac gaagcattaa aaatttataa tgtattaaaa
    98641 actgaagata tagaaaaatc taggattcag ctatcttata tagtaggtag ggatactact
    98701 aatttaagtg aacctgaaat aataagagca actgtagaaa ctgttgcaga aaatactgta
    98761 gatggtataa ttgcgcctat gttttatgca tttattggag gagcaccact tgctatggcc
    98821 tataaagcta tcaatacatt ggactcaaca gttggatata aaaataaaaa gtataaagat
    98881 attggttttg catctgctaa gattgatgat atagccaatt atataccagc tagaatatct
    98941 gtcattctta tgacaattgg aagtttcttt ttaaattata actataggaa ctgttttaaa
    99001 atctctatta gagatagaaa gaatcataaa agtccaaatt gtgctttttc agaaggtgca
    99061 gtatctggtg ctctaggaat acaactagga ggtaccaacg tttattttgg ggaaaaagta
    99121 tataagccaa caatagggga taagcttagg gagatagata aagaagatat aattaaaact
    99181 aataaaatta tgtatgcatc ttcatttgta tctatgttgg tatttacact aatatatgtg
    99241 ttatgtagga tgattttaat taacttaatt atataaatta ttgtttgtta acttaagggg
    99301 gacatagatg aaggatttag ggcatggtgc caatgtagat gaaatggcaa gattatatgg
    99361 gaaaaaccca aaagaaataa ttgattttag ttcaaacata aatccaaatg tattaccaaa
    99421 tttagagaga tacattttaa aaggattaga agagtgtagg aattatccag atataaatta
    99481 tacaaacttg agagaaaata tatcaaaata tatagatata aatccagact ttataatacc
    99541 tggcaatggt gcaacagaag tgatttattt gcttatgaag agtattaaaa aaaaattggc
    99601 tataataaat ccgacttttt cagagtatag aagaagtgct gagttaaata atttagatat
    99661 tatagattta gagttagatt tagaaaataa ctttaaactt aatatagata taataaaaga
    99721 aaatataaaa agatttgata gtttatttat atgtaatcct aataatccga gtggtaatgt
    99781 ccaagattta aaggaacttg ttcatttact agataaacat aataaggtat taataattga
    99841 tgaaactttc atggaatttg ttgaagatga aagtaaatat agtttggtta aatatataga
    99901 atcaaataaa aatatattta taattaaagc tgtgactaag ttttttggta tgcctggttt
    99961 aaggctagga tatggtctta caagcaacac tgaaattatg aacaaaattt atgaacacaa
   100021 agagccatgg actatcaatt cttttgcaga tatactatcc aattttattt ttgaagataa
   100081 agagtatatt aaaaatagta aagaatatta catagaagaa agagaatata tgttacaaga
   100141 gttgagaaac ataagaaata taaaggtcta tgatacagat gctaatttta tcttgataaa
   100201 aatatataaa aaaacaacta aagaattgaa aaaagatttg tttaagcaag gaaacatatt
   100261 agttagagat gcgtctaatt ttataggatt agatgatagt tttataagag tggctataaa
   100321 atctcatgaa gataacaaaa ttcttataga aaatatgaaa aatttattgg gtgattaaaa
   100381 tgaaatctta tggaatatgc ccagcatctt gtggagaatt tgtacaaggt ataattgatg
   100441 atgaagaata tttatgttca tatgcaatag atatgtattc taaagtgtat attgaagaaa
   100501 aattagtaga tataaattta gggagatata agtcaaggtt agcaattgaa aaggtatttg
   100561 aaaaatttaa tttacctaaa aaatatacta aaaatatttc tttaaatata aatagtaaaa
   100621 tacctgttgg aaaaggaatg gcaagttcaa ctgctgatat aggagctact ataaaggcaa
   100681 cattatcatt gatagataaa gatttaagta gcgaggagat ttctaaatta gcagcagaaa
   100741 tagaaccaac agattctata tttatagata aaaatagtat atttaatcct ctaaatggaa
   100801 ctgttataaa atacctagga aatctgacca atgcgaaagt tgtaatactt gaaccaaaca
   100861 aagtactaga tactatgaaa attagattga ggcaagatta taataaatta aaagtagaga
   100921 ataaagaagt aataaaaaaa tcatttgctc ttttagaaga aggattgaaa aaaaataatt
   100981 tatctctagt tggagaggct tgtactttga gtagcttagc aaatgagaat atagaaaaaa
   101041 aagagtattt aaatgaaata ataaaaatat caaaaaaata tggagcatat ggagtaaata
   101101 ttgctcacag tggaactgta gtaggtattt taattgataa gtttatgaat gataaaaaga
   101161 tgatagatgc cttatgtgaa tctaacataa attctgtcta taataaaatt tatacacaaa
   101221 acataattaa tggaggaatt aagggggaaa tagaatggaa tacataaaga atcctatgaa
   101281 aatagaagaa aaaagttttg aattgataca agaaataata gacgaaatta gaccagatta
   101341 taaatttaag aataatatcg aagaaaaaat aataaaaaga gctatacata ctactgctga
   101401 ttttgattac ttagatatat taaaaatatc agaagaagca gttgatagta taatagatgc
   101461 attaaaaaat aatgcaagta tttatactga tacaaatatg gctcttagtg gtataaataa
   101521 aagaaagtta gaatcattag gatgtaagta taaatgttta gtggcagatg atgaaactgt
   101581 aaaattagct aaagaaaaag gaattactcg ttctatggca gcagtagaaa ttgcagctaa
   101641 agaagaagga agaaaaatat tcgttttagg taatgctcca acagctttat ataaagttat
   101701 ggagatgaaa tcagaaggta gattagattt agatgcagtt ataggagttc ctgtaggttt
   101761 tgtaggagca caagaatcaa aagatgaagt tgaaaaaaca gatataccat atataatttc
   101821 taaaggtaga aaaggtggca gtaatttagc agcagcaata gtaaatgcta tattatatac
   101881 tatgtaggta cctatatgga agaatatgta tatatagatg gaaaaaaata tagaagagga
   101941 tatactacag gttcatgtgc aacaggtgca tctaaggctg ctgtatatat gttgattaca
   102001 aaaaatagaa ttaatactat aaatatagat actcctaagg ggataccatt actactaaaa
   102061 gttgataata taaatatttc tgatacattt gtggaatgtt ccataaaaaa agatggtgga
   102121 gatgatatag atgcaactca taccatggat atttatgcaa gagctgaaat tgtagctaaa
   102181 aatgataaga acaaagggta tcttactcta aaggatatag atagtttaag caccaatagt
   102241 gagtgtaaaa gtgagctcta taagtttatt agggtttatg gcggaactgg tattggtgtg
   102301 gttacgaaaa aagggcttag tgtggatgtt ggtaagcctg ctataaatcc gactccactt
   102361 aaaatgataa atcatgaaat aagaaagctt ataggagata attttgaatc tatacttgga
   102421 aatgataaag ttttaaaaat aacaatcttt gctccacaag gagaaactgt agcaaaaaaa
   102481 acatttaatc caagattagg tatagttgga ggaatatcta taataggtac aactgggatt
   102541 gttgaaccca tgagtgatga tggttggaag aagtctctat ctatagaact tcagatgaaa
   102601 aaagaacaag gcttagataa aataatattg gttccaggaa atcatggaga acagtttata
   102661 agagaaaaat taaacttaga tattaagtat gtagttaggg taagtaattt tataggctat
   102721 atgattaaag aagctcaaag aataggatat aaaaaaatct taatggcagg ccatataggt
   102781 aaatttataa aagtatctgc tggtattttt aatactcata gtaaggtagc agatgctaga
   102841 agtgagatat tagtggcaaa cttagcttta atgggagcta gatacgaatt tttaaataaa
   102901 ataaatcaat gcgtaactac tgaagaagct gtagagctaa taaataatag tgaatataga
   102961 gaagtatata atatattaag taataaatgt agggagagag taaagcaata cttgaatgaa
   103021 gattcagatg atattgatgt ggaagtaatc atattttcaa tggataagtc tttattaggt
   103081 aaatcggata atacagatga tttagtggag gtctttatat gataaacata ataggtctag
   103141 gacctggtaa cttagactat ataacaaaaa aaggtgaaaa tttaatttcc acttctgatg
   103201 tattaattgg tggtaaaaga aatttagagt ctataaaaaa ttttgaagga gaaaaaatag
   103261 ttctggattc taatttaaga gaaattatag agtacataaa taacaataaa gaaaagcaaa
   103321 tatcaataat agcatctggg gacccattaa tatatgggat aggtagatat ctatctaaaa
   103381 atattgataa taagatgtta aacatggttt ctgggattag ttcattacag tacatatttt
   103441 caaaaatata tgtagatatg aatgatgtat atattacaag tagtcatggc aaagtaccag
   103501 attttgatta catattatca cataaaaaag tatgtatggt tacagattca aagataggac
   103561 caaaacaaat aagtagagaa ataatagata gaaatctaaa taaaattatt gttgtaggag
   103621 aaaatctgtc atatgacaat gaaaaaataa caatagcaaa accagaagag ataataagaa
   103681 tggacaattt tgatatgaat gttgtggtga ttttagatga ggaatagtga atttataaca
   103741 ggtaaagttc caattactaa ggaagaggtt agagcaatat caataagtaa gttaaattta
   103801 gtaaatgcta aaaacctcat agacataggg gctggtacag gaagtgtaag tgttgaagct
   103861 gcatataatt atcctaattt aaaggttata tctatagaga aaaatgatga tgctatagag
   103921 cttatagaga aaaataaaaa aaaattcaac ttaaaaaata tagaagtgat aaaaggatat
   103981 gctccaatta atctagggtt aaatgttaaa gttgagtcta tttttcttgg gggaacagga
   104041 aataatctgg aagaaataat agaatggtct aaaaaaaatc tcgttacagg aggaagactt
   104101 gtagctaatt ttataataat agatacattt aatcaaactt taaaactgct taaaaagcat
   104161 ggatttaaag aaatagatgt atgtgttttg aatgtttcta aactagagaa actaggaaaa
   104221 ggtgaatatt ttaaaccact aaatcctata tatataatat cttgtgaaaa aggggaaaat
   104281 gatgatgaat aaagtacatt ttgtaggagc aggacctgga gataaagagc tgataacatt
   104341 aaaaggatac aaattattaa gtaatgcaga tgtagtgata tatgcaggtt cacttgttaa
   104401 tcctgaactt ttggaatact gtaaagaaga ttgccaaata cataatagtg cacatatgga
   104461 tttacaagag ataatagatg ttatgagaga aggtatagaa aataataaat ctgttgtaag
   104521 actgcaaaca ggtgattttt ctatatatgg ttcaattaga gaacaagtgg aagatttaaa
   104581 taaattgaat atagattatg actgtactcc aggagtaagt tcttttttag gggcagcatc
   104641 atctttaggg gttgaatata cagttccaga aatttctcaa agtgtaatta taacaagaat
   104701 ggaaggcaga actccggttc cagagaaaga atcaatacaa tcatatgcta agcatcagac
   104761 atctatggta atatttttat ctgttcaaga aattgagaaa gttgtgtcta aattactgga
   104821 aggtggatat ccaaaagata ctcctatagc agttatatac aaagcaactt gggcagatga
   104881 aaaaatagtt aaaggaactt taagtgatat agctgtaaaa gttaaagaaa acaacataaa
   104941 taagactgct ttaataatgg ttgggagatt tttaggagaa gagtataata attcaaagtt
   105001 atatgataag gatttcaaac atgaatatag agggtaatat tgctataatc tgtattacag
   105061 aaaatggaaa aaaattagct ctaaagataa attctctagt aaataactgt gttatatatc
   105121 atgtaaaaaa taaaaaaagt aatttagttg ttgaagaaac atgtgtgcat acagttcaaa
   105181 agaagttaag tgattttgta ggagaaatat ttagcaaatt tgattacata gtatttataa
   105241 tggctacagg tatagttgta cgaagtatag caccatacat aataagtaag tttagtgacc
   105301 ctgcaatttt ggtaatggat gaaaaaggca aaaacgtaat aagtttattg agtggtcata
   105361 ttggaggggc aaacagcttg acacagtaca taagtagtct tattgatgct aatccagtaa
   105421 taacaacagc tactgatata aatcaaaaag cttccttaga tatgatagca aaaaaactaa
   105481 atgcacatat tgataatttt agagaaagtg taaaagaagt aaattctatg ctagtgaata
   105541 ataaatgtgt tggattatat attgatgaag gatatcaagt tgatactagg ggatttaaac
   105601 ttatagaaag taaggatgag ttaagagata gtttttcaaa cacatatata gaaaaattag
   105661 ttgtagtaag caataaaata aattgtattg aagatattga gaatttaaac ctagataata
   105721 aatttgaaca ttgctatagt accaaaaaaa taataaaggt aattccaaga gatatagtga
   105781 taggaattgg ttgtagaaga aacacagata gtgaactgat gtataattca cttgaagagt
   105841 ttcttaataa acaaaatata gatattagag caataaacac agttggaagt atagaactga
   105901 aaaaagacga aaaagcaata attgaattat caaaaatatt gaaggtgcca tttaaaacat
   105961 tttcagtaga tgaaatatct aaggttgata atctttttga taaatctgat tttgttaaaa
   106021 aaaatgtggg cgtgtattca gtagcagaac ctgtagcata tcttttgagc aaaggtaatt
   106081 tgttagttga aaaacataaa tataaaggaa taactttttc agtggggaga ataaaaatat
   106141 gatatacgtt gtaggaattg gaccaggaag taaagataca atgactttag aagcaataaa
   106201 agcaatagaa gattctgagg ctattgttgg atataagact tatataaaac taatagaaga
   106261 atttatacaa gataaagaaa taatccaaaa tggtatgagg caagaagttg atagatgtaa
   106321 acaagcagta gaggaagcaa aaaaaggaaa aaaagtagca gttgtaagca gtggtgatgc
   106381 tggaatatat gggatggcag gtcttatttt agaattaata tctaaagaag atgaagatat
   106441 aaaagtaaag gtaattccag gtgttactgc aagtattggt gcagcagcta tacttggagc
   106501 tcctattatg catgatttct gtcatataag tttgagtgat ttaatgactc cgtgggaagt
   106561 aattgaaaaa agattgagat tggccgctga agccgatttt gtaatttgtt tgtataatcc
   106621 aagaagtaag ggaagaagtg aacatctaag caaagcattt aaaataatgg gtgagtttaa
   106681 aggtggagat acacctgttg gagtggtaaa agatgtaggt agagaaaaag aagaaaagtt
   106741 tgtatgtact tttgaaaata tggactttga aaaagtcgat atgactacaa tggttataat
   106801 aggaaataag tctacctata ttaatggtga attgatgata actccaagag gttatacagt
   106861 atgattttaa ttttaggagg aacttcagat agtttatcta tatgtgataa gataaatgaa
   106921 ctgaaaaatc aaccatatat cctttcagtt actacagatt atggaaggga acttgcacaa
   106981 caacatgcag agaatgtaat tttaggtaag cttggaaaag aagatatgct aaaatttata
   107041 aaagataata atatagtaaa aataatagat gccactcatc cttatgcagt tgaagtatcc
   107101 aaaactgcta ttacttgtgc aaaactattg aatatagatt atataaggtt tgagagaaaa
   107161 tctttaattg aagaaataga ttatgataat aaatttatag tagatacaat tgaagaagcc
   107221 tgtgaaatag caaataaaat agggacaaat atattcatag gaacaggaag taaaaatttg
   107281 aacagattta tagaggaaat aagtgataaa aatttagttg ttagagttct tccaacgagt
   107341 gaagtcatat taagttgtga aaaattaggt ttaagtgcag ataatatagt agctatgaaa
   107401 ggtccattta gccagtatat aaatgaagaa acatataaac attacaatat agatttagtc
   107461 ataacaaaag aaagtggaat agctggtggg ttcttagaga aggtaaatgc atgtaaaact
   107521 ttcaatatac cagtaataat tataaaaaga gaatcgatta actacgaaaa tgtatttaat
   107581 gacataaata aaattaaaga catacttgtg taatttttag gaggagaata tatgaagaaa
   107641 gccttattag tagtgagttt tggtacaagc tatcatgaaa cgagagaaaa gacaatagat
   107701 atatgtgaag ataaaataaa aaattcgtta aaaagccatg atttttttag agcttataca
   107761 tcaaatatga taataaataa aataaaaaaa agggatggaa tagaaataga taatccaata
   107821 caggctttag ataaaatata tgaacaagga tatgatgaag taataatcca aacacttcat
   107881 attatatgtg gcgaagaatt taacaaatta aaagaacaag tagatggata ttcttctaaa
   107941 ttcaaaaaga ttgtattagg aagacctttg cttacacata ttgaagatta tcaggaagct
   108001 gtagaggcaa ttaagcatca aattcctcat atggaaagta atgaagcagt agtatttatg
   108061 ggacatggaa cacttcatga atcacattca gcgtatccag cattggaata catgcttagg
   108121 gataatggaa taaatgctta tgttggtaca gttgaagggt atccagaaat agaacatgta
   108181 ataagaagat taaaagaagg aaatataaaa actgtaaatt tgatgccatt tatgttagtt
   108241 gcaggagacc atgctataaa tgatatggca ggagatgaag aagattcttg gaaaacaata
   108301 ttagaagaaa atggatttaa cgtaagagtt catttaaaag gactaggaga aaattcttat
   108361 atacaagata aatttgttcg tcatgctgta aaatgtgcag agaatgcgaa gttctatggt
   108421 ataggagcag ggccaggaga tggttcttta cttacgataa aagcggttaa tactttaaag
   108481 aaactggata ttctatacac tccagaatca aaaaagggtg gagatagtct agctttatca
   108541 atagttagtg aatatttacc tgaatcagtg gaaataaagt ctaggcattt cccaatgagt
   108601 tttgatggga atgaaaaaag tttagcttgg gacaatgtgg cacaagaaat tatatcagat
   108661 gttaatgatg gtaaaaatgt aggatttgtt acattaggag accctatgat ttatagtaca
   108721 tatgtatata taatgaaaag aatattagga gaggtagaag tcgaaactat accaggaatt
   108781 tcatcatttt ccaatatagc atctaatcag aattttccat tagtcatgga taaagaagct
   108841 ttagtggtaa ttccttgtac tatggaagat gataagatag agtatgcttt gcaaaattat
   108901 aattcaatag tattgatgaa agtatataaa aattttaaag aaataataaa aaaattagaa
   108961 aagtatgatt taatagaaca tgctatttta gtaagtaatt cttcccaaga aagcgaaaat
   109021 gtgtttacag atttaaaaga agcgcacctt gaagataaga tatcttattt ttctactata
   109081 ctagtaaata aagataataa aataaaatag gtgaaagcaa tatttaaagg agacttaata
   109141 tgaatatagt ggttggaact aggggtagta atctagcgct tatacaaaca gaatgggtaa
   109201 taaatgaatt aaaaaagaag tatccagaaa ttagttttga gattaaaatc ataaagacaa
   109261 aaggagattt aattcaaaat gtttcactgg ataaaatagg tgataaagga ttgtttgtca
   109321 aagaaataga gcaacaatta ttagatggaa aaatagacat agcagtacat agtatgaaag
   109381 atatgccttc ttatttagca gatggactaa aatttgctca tacgcctaag agagaagacc
   109441 caagagatgt tctaatactt agagaaggat ataaaaattt agatgattta cctcatggag
   109501 cagtaatagg aacaggaagt aaaagaagga agtttcaatt attgaaacaa agacctgatt
   109561 taaatatagt acaagttaga ggtaatgtgg aaactaggat acgaaaaata aaagatgaaa
   109621 acatgcatgg tattgtgttg gcagcatcag gaataattag agctaatttg caagataaaa
   109681 taagttctta tttacctgtt gatgttgtta ttccagcacc tgcccaagga gctttagcta
   109741 tagaaattag aagcaatgat agtgcgatag agggaatagt aaattcactg aaggatgaga
   109801 atacagaaat ccaaatattg gctgaaagag gttttttaga tggtgtaaat ggaagttgtc
   109861 atattccaat ggcagcttat tgtgaaataa tccaagataa aatacatctt actggcttat
   109921 atggagattc agaaggaaaa aaagtagtaa tcaagtctat agatggagat atttcttctc
   109981 ctagagagtt aggtttaaag ttagctaaac ttgtattgaa ggagtatgag aattatgaag
   110041 ggtaaggttt acttagtagg ggcaggacca ggtgattata aactcatgac tttaaaaggt
   110101 ttagagtgta taagaaaatc ggatgtaata gtatatgata gattagcaaa tagtagttat
   110161 ttaagagaag ctaagcctga ttgtgaactt atatatgtag gcaaggcatc aagtaatcat
   110221 attttgccac aagaagatat aaatagaatt atagcagaaa aagctaaaga aggaaaaatt
   110281 gtaactcgtc ttaaaggagg agacccttat gtatttggca gagggggaga agaagcagag
   110341 actttagtag atgaaggtat agaatttgaa gtagttccag gtataacttc tgctatagga
   110401 ggtttatgtt atgcgggtat tcctataaca catagagacc atgcttcgtc atttcatgta
   110461 ataacaggtc atttgaaggg tgatgatagt ggagaactaa actggaatgc tcttgcaaat
   110521 aacaaaggta ctcttgtgtt tttgatggga atttcaaacc tagagaaaat aagtgaaaac
   110581 ttaatgaaag aaggaaaaga taaggataca cctgttgctc ttataagttg ggcaactaga
   110641 tataatcaaa gagttgttac atctacactt gaaaatgttt atgaaactgc tattaaagaa
   110701 gaagtaaaac cgccaactct tatagtagtt gggagtgttg taggacttag agaaaagttg
   110761 aacttctttg aaagtaagcc tttatttgga aaaagtattg ctgtaactag gtctagaaat
   110821 caaaatagtg tattggtaga aaaaataatg gatttaggtg gaaatcctat agaaatacct
   110881 actattaagg tagaaaaaat acaaaataat attaacttag aaaatgaaat aaaaaatata
   110941 aataagtata attatctaat actaactagt aaaaatgccg tagaaatctt ctttgaaaag
   111001 atatatgaaa tgaattttga tttaaggata ttatctaatt taaaggtatg tgctataggt
   111061 tctgctacat caaatgagtt gaaaaaaaga ggaattatag cggatatagt accaaaaaaa
   111121 tttgtagcag agtcactgta tgaagagtta gcgcctattt taaatgatga cgataatata
   111181 cttataccta gagcagaaaa tgctagagat tatctggttg acaaacttaa ggctatttgt
   111241 gaagtaacag aagtacatat atatagaaca gtaatagatg aggagaaaaa aggtgagata
   111301 ttagatattt taaattctaa tgacatagat tatataacat ttactagttc ctcaacagta
   111361 aataatcttg ttgaaattat aggaaaagaa aatattaaaa tattagaaaa aataaaaaca
   111421 ctatctatag gtcctataac tacagagaca ataaatagtt tgggaataaa attgtacaaa
   111481 gaatctgcta catctactat agattcgtta gtagatttaa ttataaaaaa ttaattttgg
   111541 aggcttgaat atgttaagaa gaccaagaag actaagagca agtaaagcaa tcagaaattt
   111601 ggtaagagaa actaaggtga atgtagaaga cttaatatat ccactgtttg tagtagaggg
   111661 ggaaaatata aaagaagaaa ttaaatcttt accagatgtc tatcatttat caatagatat
   111721 gctagaagac gaaataaaag aaataagtga gttgggaatt gaacatataa tgttatttgg
   111781 tgttccagat gaaaaagatg agtgtgcttc acaagctttt aatgataatg gaatagttca
   111841 aaaggcaatt agaaaggtaa aagaaataaa ttcagatata aatgtaataa cagatgtctg
   111901 tatgtgccaa tatacaagtc atggacattg tgggatttta actgatactg gttatgttga
   111961 taatgataaa tcattggagt atttggctaa aatatctgta agccatgcta aagcaggagc
   112021 tgatatggtt gctccatctg atatgatgga tggaagaata caagcaataa gagaagcttt
   112081 agatgaaaat ggattagaaa atgttggaat catggcttat agtgttaagt atgcttcaaa
   112141 ttattatgga ccttttagag aggcagctca ttcagcacca tcttttggag atagaaaaac
   112201 atatcaaatg gacccagcaa atagcaatga agctataata gagactgaac ttgatatatt
   112261 ggaaggtgca gatatactta tggttaaacc tgcattatca tatttagata ttataagaag
   112321 agttaaagat aattttgact tacctctagc agcttataat gtaagtggag aatactctat
   112381 gcttaagttt gctgtgaagg aaggtttatt aaatgaagat gtaattcttg aatcaattac
   112441 atctataaaa agagcaggag cagatataat aataacttat tttgctaaag acattgctaa
   112501 actgttgaaa aaataaattc tagcagtata aagaatatca aaaagtatag gaattgtaat
   112561 taactattat acagtagttt tgtttagaca tttattgaag aataatagaa actaatttgc
   112621 aatttctata ctttatttat gtaactaaat aaaatttgga ggtagaagat gctatatcct
   112681 attaatttaa aattagatga gttagatgta gttataattg gtggtggaga agttgcatat
   112741 aggaaatgta aaaatttttt agagtttaac aaaaatgtta ctatagtttc taaacaaatt
   112801 ttaaataaat tttatgattt aaaaggaaat ataaaaataa taaaagatga ttataaagaa
   112861 atatatataa aagatgcttc tgtaataatt gctgccacaa ataatagaga gttaaatatg
   112921 gaaataggat tatattgtaa agcaaaaaat aagctagtga atgtagttga taatatagaa
   112981 atatcgaact ttactgttcc atcatatata aaaagaggag atttacttat aagtgtgtct
   113041 actgggggca agagtccttc attatcatca aagataaaaa aagaaattga agagagatat
   113101 actgaagatt atgaggagta tttaaatgta ttaggagata taagaaaaaa ggttataaaa
   113161 aaatacgaag ataaaagtaa aagaaaagat gtgcttaata tgttggtaac acttgaccta
   113221 gaagaactta agaagtttga tatataaaaa ataaaataaa aaatgttaaa aaggtgttga
   113281 cggat
//
LOCUS       DAMJVI010000004        84922 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.004, whole genome shotgun sequence.
ACCESSION   DAMJVI010000004 DAMJVI010000000
VERSION     DAMJVI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 84922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.004"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     regulatory      complement(44..141)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            350..739
                     /locus_tag="PC545_000335"
     CDS             350..739
                     /locus_tag="PC545_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2455133.1"
                     /translation="MKQQEVVNKLRRGELMLMEYLWRKNSILSKKEIIEAMKEKYKWR
                     KSTTEILLKRLVKMKTLKKKRIGFQFSYEVLVTKKEYLNAIKEDRKVTKYDSFFIRMF
                     TTMHKKEKMTEEQIKKYIENMEKIGKE"
     gene            833..1216
                     /locus_tag="PC545_000336"
     CDS             833..1216
                     /locus_tag="PC545_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2455134.1"
                     /translation="MGFKKLPDSELKVMKFIWGLDGNKVASGEVVEAMKQIYDWNKNT
                     TLTILSKLAKKNYLYSQKTSKCTYYTIAVREKDYLKVETKKFFSFFHNNSLKSFFTAL
                     NDEENLSDDKLDMLEEWVKNWEEDE"
     gene            1895..3325
                     /locus_tag="PC545_000337"
     CDS             1895..3325
                     /locus_tag="PC545_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2455135.1"
                     /translation="MILNQIFIEAGGIMRNKIIIGITFSLVLLMSGCASKQTAEINVL
                     ATTDLHGVIPYELTSYVKEERKKDKNLTLVDAGDFFDSGEVFGSSMDSYFSARYKDGD
                     NNTESYIETPISKDMKEVGYDVAVLGNHEFISNNKFYLDNMISDFEKQKINILSANTY
                     KKNGDSYTKPYIIKNINTPEGNVKLGILGLTIKEVGERKQWKDGKLVDSKSLELKDQE
                     GYNGELYMNDLVEDAKKWVGIMEKEKPDIIVAVAHTGEKPKKPKNPGNRIQDLAQQVD
                     GIDAIVAGHNHVQIEQHDYKNKSGENVIVTEPGKHGECISKINFKLEKNNDKWNIVDK
                     SSKLIKFEKNQESDKFGEFISSINKVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDI
                     YKTIGYKWRNISSPKGDDMVQIVVMNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVT
                     IYPNKNDKFKIEKGKQEYSINLKHIQ"
     gene            complement(3473..3823)
                     /locus_tag="PC545_000338"
                     /pseudo
     CDS             complement(3473..3823)
                     /locus_tag="PC545_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3818..4339
                     /locus_tag="PC545_000339"
     CDS             3818..4339
                     /locus_tag="PC545_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2455136.1"
                     /translation="MFSIGGGYAIILLIQEKVVNQTGWISEKMFTDIITISQMTPGPL
                     AVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKHQNSTYIFEILNGLKS
                     ASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFVTLLIVRKWKINPIVI
                     IIISGILGIFFYL"
     gene            complement(4468..5262)
                     /locus_tag="PC545_000340"
     CDS             complement(4468..5262)
                     /locus_tag="PC545_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2455137.1"
                     /translation="MIFCNSFITRKSSMNIVVTHTPIVTTLEMQKAYEPLAKHNVNIF
                     AFDFSGTGKSDGYEKDFSRNSIVKDLDSVITYIEKNYSSNIHLYGNTGIGGMFAQYYA
                     CSDNKLKSFAQFACVDYKNTTGVGYTYLTVKLLNFFFKFLPNFHITVKPPKYKGYNEV
                     YDNEFYKIFEKKNPHIWKNSTKIINTMLECFIAKDSKIKDGVNIPTLVFKTLHDRYFK
                     TQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVENQ"
     gene            complement(5486..8914)
                     /gene="uvrA"
                     /locus_tag="PC545_000341"
     CDS             complement(5486..8914)
                     /gene="uvrA"
                     /locus_tag="PC545_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2455138.1"
                     /translation="MKINNYKNQSIITNPKKFENKYQDLPKTPIELLKVVQSLVIHGD
                     QGKLYGISFNKQQSDEELLRTIPQMLKRIFEINSNPLTIPRNPKQRLVGMCRDYSLLL
                     VSLLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTLTKRWIRIDAQIDDIQKNYF
                     QINFDTHDVGKTDGFLTGSEAWIRCRQGHAHQDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNIKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELGNPLELEKKFKFTKSINKSIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNQITVITGVSGSGKSSLAFDTIYEEGKRRYFENLSNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGSQNPRSTVGTLTSIWDYLRMLFVSTGKSYCPYCKIPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLKGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESKGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELPNQLQKNELSLVNDILTELKIRVSYLLK
                     VGLSYISTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPSAGTKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPDKNYTEFTGFDAIDDFILMDQTPIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYMADQWVTCSEC
                     QGKRYQKEILSIQYKGKTIADILDMEVAEAKTFFSDCSDIYRKLSLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGINGGEIVASGTPDEIKINPNSITGHFI"
     gene            9233..11467
                     /locus_tag="PC545_000342"
     CDS             9233..11467
                     /locus_tag="PC545_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2455139.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISNKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDVGI
                     IDLEGKAEFLSGKKFDLKNTVSYKKGIEGKEYSEVIESMGFVLYSVPIFNDNHEVSSI
                     LIGVSNKESMNEILNIDNFNGKGTIEIINSKGKPLFIGKNTELIKDLSHKYKSSGDEP
                     WAQKMLEDFKNDKSGNITITSSKGVQCLLTYHPITRGLNDWHFLLIVPEDAVLGELNK
                     LNSFTITMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSMLVKP
                     LISESPDSTYMMIAMNIKDFKLINDCFGSEKGNMTLKHLYNILYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADTNYFNKNRENPYFLRLSVGIYTIENHNEDLIT
                     IQDHANTARKSFNKTHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKHICNKYNVPTCLIDLELTESIFLENPEAID
                     IIEDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFENFAYENNKIK
                     NIHY"
     gene            11723..13753
                     /locus_tag="PC545_000343"
     CDS             11723..13753
                     /locus_tag="PC545_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2455140.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKSVIQKMNKGVLIIPDDFQVVCINNNEYVFSLTERVSIMKIISMFKIDNLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKSFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGIYLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESASLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLSQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLVNDYQINIVESIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     IICQELGYSDERILFRQLNLSDLIGIDTIRIIDGDMKWEDAVKFSSNILANCDFVSAQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVKIIF
                     CLASRDKKEQIPAIINLTKMVYKTNFIAMLESAKTKEEAEVLIHKYEKEVTV"
     gene            13750..14202
                     /locus_tag="PC545_000344"
     CDS             13750..14202
                     /locus_tag="PC545_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2455141.1"
                     /translation="MNKLVNVKCSFKIHVNSKEEAIISLVDVVSKEGYLIDKNQFLKD
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEEED"
     gene            14204..15259
                     /locus_tag="PC545_000345"
     CDS             14204..15259
                     /locus_tag="PC545_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDF2455142.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLVGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMAGSI
                     VGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAVLMKLVKKDVKEDE
                     EIEEDLDESIELIFEEL"
     gene            15326..15634
                     /locus_tag="PC545_000346"
     CDS             15326..15634
                     /locus_tag="PC545_000346"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDF2455143.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKDIDEATCVILTNDVEIRNMERFKGKKVVRMSVSEIIKKSDALIKKIKDTFQ
                     "
     gene            15738..17003
                     /locus_tag="PC545_000347"
     CDS             15738..17003
                     /locus_tag="PC545_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HDF2455144.1"
                     /translation="MKKLPIKTVVQRLLNLQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKVADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPDIGYEVGTEETNGGLTSTETYETFITRLKVELDNRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SQIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVEKTSNIRHVLLVNAIECGRWRK
                     WVVGEQKNLTTEEIFKDEVLSNEILDIAGHYTFNNDDVKKEIEVLYDNLSKNNIDGQR
                     FVVDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            17091..17807
                     /locus_tag="PC545_000348"
     CDS             17091..17807
                     /locus_tag="PC545_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2455145.1"
                     /translation="MINLIFDVDDTLYNQLTPFYTAYNKVFSSIKDISIEDLYMSSRK
                     YSDEVFHMTENGEMPIKEMHIYRIMKAFEELGNSITEKDAQSFQDEYIYQQSQITLIP
                     EVERILNFSKERNINLGIITNGPSNHQRMKLKQLNIENWVDKSNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYVGDSYRNDVLGAKKAGWKSIWLNHRGHEVEELFYKPDFV
                     ILEHKDLISLFIKICSYKNM"
     gene            complement(18002..18295)
                     /locus_tag="PC545_000349"
     CDS             complement(18002..18295)
                     /locus_tag="PC545_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HDF2455146.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWVKPYDKPIQQLTCSYKESQNVID"
     gene            complement(18301..18516)
                     /locus_tag="PC545_000350"
     CDS             complement(18301..18516)
                     /locus_tag="PC545_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455147.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAMQQFIVENEILISEEV"
     gene            18965..19363
                     /gene="tnpA"
                     /locus_tag="PC545_000351"
     CDS             18965..19363
                     /gene="tnpA"
                     /locus_tag="PC545_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDF2455148.1"
                     /translation="MYKSNKNIVYDCKYHIVFCPKYRKPVLVGEVEKMLKEILPYKAD
                     ELGAEIIEMETDKEHVHLLISCDPQYGIHKVVKGLKGFSSRVLRENFPHLKSSMPSMW
                     TNSYFVGTVGSVSLEVVKQYIENQQVRTKK"
     gene            19360..20523
                     /locus_tag="PC545_000352"
     CDS             19360..20523
                     /locus_tag="PC545_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2455149.1"
                     /translation="MIKTHRVKLNLTRTQFELVREKQMESANCWNYIVNLSKEYYFEH
                     KQWIKKNDIQKLIKGKYNLHSQTIQAISDKFDANRKTISELRKKGNTKAKYPYKTKKF
                     YIIPFKASAINRNAKGNLKLSMSKGRYLELDFNVENIKTAEIVWRNGYYLYYTFDNEL
                     NGVVAKGTNTVGVDLGEIHSIASVTNEGVGLILSNREGRSIKQFRNKMYAYISKRLSK
                     CKKGSRQSKKLWRLKNKIRSKTDNQLMNLYHQTTRKFIDFCVEQKVCEIVLGDIKGVE
                     KDTKKKKRLNRVNRQKISQMEYGRIKDYIKYKAKEQGIEVKLVKENYTSQTCPKCSKK
                     HKPTGRTYSCTCGYETHRDIVGAWNILNKKHKYGLVDFRINHKQPINLKVSTV"
     gene            complement(20907..21419)
                     /locus_tag="PC545_000353"
     CDS             complement(20907..21419)
                     /locus_tag="PC545_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2455150.1"
                     /translation="MLEVIKNRRSIRTYIDKNIEEDKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKQLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWAGVFPKNKVVNKVRQTLDLPLKLVPYALISIGYSEEKNEFIDRFDEN
                     KIHRNVYKNR"
     gene            21869..22345
                     /gene="purE"
                     /locus_tag="PC545_000354"
     CDS             21869..22345
                     /gene="purE"
                     /locus_tag="PC545_000354"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDF2455151.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     SIFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            22351..23049
                     /locus_tag="PC545_000355"
     CDS             22351..23049
                     /locus_tag="PC545_000355"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2455152.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            23061..24428
                     /gene="purF"
                     /locus_tag="PC545_000356"
     CDS             23061..24428
                     /gene="purF"
                     /locus_tag="PC545_000356"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDF2455153.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKENELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            24422..25498
                     /locus_tag="PC545_000357"
     CDS             24422..25498
                     /locus_tag="PC545_000357"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDF2455154.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHDIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVDERELHKSFNMGIGLVLIVDKENADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            25492..26085
                     /locus_tag="PC545_000358"
     CDS             25492..26085
                     /locus_tag="PC545_000358"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDF2455155.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQGAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            26078..27610
                     /gene="purH"
                     /locus_tag="PC545_000359"
     CDS             26078..27610
                     /gene="purH"
                     /locus_tag="PC545_000359"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDF2455156.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKEKP
                     TDAQLKDMEFGMRVVKNMKSNAIAVVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            27621..28871
                     /gene="purD"
                     /locus_tag="PC545_000360"
     CDS             27621..28871
                     /gene="purD"
                     /locus_tag="PC545_000360"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDF2455157.1"
                     /translation="MKILVVGGGGREHAICWKLSKEKNVEKIYCAPGNAGIANVAECV
                     NIGDTNIEELLKFAKENEIGLTIVGPEVPLVMGIVDEFEKEGLRVFGPNKKCAQLEGS
                     KAFSKEFMIKHNIPTAKYKEYTNLEEAISEIDSFGYPVVIKADGLAAGKGVVIPENRE
                     DAIATLKEMMSDKKFGAAGDKIVIEEFLKGIETSILAFVDNDTIVPMASAKDHKKVNN
                     YEQGPNTGGMGTFSPSEIYTEELANKVKETVLEKTLEGFKKDGLNFKGILFVGLMITE
                     DGEKVLEYNVRFGDPETQSVLFRLETDLHEIMEAILDNKLKDIEINYSDDEAVCVMLT
                     SGGYPDSYEKGKIITGLENLDDDIVVFHSGTKMFDGNLVTNGGRVIGITAKSTRVKDA
                     AEKVYENIKKINFEGMHYRTDIGR"
     gene            28896..32702
                     /locus_tag="PC545_000361"
     CDS             28896..32702
                     /locus_tag="PC545_000361"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDF2455158.1"
                     /translation="MLNTENKDSMVRRVLVEKREGFDLEAKALKKDLVESLHIDNIEN
                     LRILNRYDVEGISEEVYENAAKTIFSEPNLDVVYYEEIPKLNDERVFAIEFLPGQYDQ
                     RGDWAAQCVQIVNQGIRPAINTAKVYILSGKITDEEFSKIKDYCINPVDSREASLEKP
                     ETLKMETEIPTTVEVLDGFIDLDENGLRTFVSEKGLAMTLGDLQHVQKYFKDTEKRNP
                     TITEIKVLDTYWSDHCRHTTFMTEIENVKIEDGKFNDIVKEAYQMYLNSRDNVYVNRH
                     KDICLMDIATVAVKELKKNGKLNDLDESEEINACSINVDVEVDGKMEKYLVMFKNETH
                     NHPTEIEPFGGAATCLGGAIRDPLSGRSYVYQAMRVTGSADPRTTLEDTLPGKLMQRK
                     ITTEAAHGYSSYGNQIGLTTGQVAEVYDENFVAKRMEIGAVIAAAPKENVVRERPEAG
                     DVIVLLGGKTGRDGCGGATGSSKEHSEESILTCSAEVQKGDAPNERKIQRFFRNKEVA
                     QMIKRCNDFGAGGVCVAIGEIADSLDINLDLVPKKYDGLDGTELAISESQERMAVAIK
                     KENKDKFIQLAVEENLEATHVATVTDTGYLRMFWNGKAIVDINREFLDTNGVKQTTDV
                     HVTKVDEENTFFSSNEIVKDVKCASMKDKFTKVLSDLNVCSQKGLVEMFDNTIGGNTV
                     LMPFGGKYQATPTQGMVAKIPVLGGETNTSTIMTYGYNPKVGKWSPFHGALYAVVESV
                     CKLVAIGGNYSTTRLTFQEYFEKLGNNPEKWGKPFSALLGAFYAQSKFEIPAIGGKDS
                     MSGTFKDIEVPPTLVSFAVDTVDAKKVVSPEFKKADSKVVMLCVNKAENDVVDFEELK
                     RNLDKVRELIHGNKVLSTYALGFAGVGEAISKMAFGNKIGFKFSEEAEKAFTDNKLFE
                     ASYGNIVLELANDDLSMLEGYNYVVIGSTVKEASIFIKGEELALDELYKAHCSTLEPI
                     FPTKTEEVKSKIETISYISQGEAKKSSLSIATPRVFIPAFPGTNCEYDSARAFERAGA
                     NASIRVFKNLTYKDIEDSIDTIVNEIKSSQIIMLPGGFSAGDEPDGSGKFIATVFRNP
                     RVQEAINEFLTQKDGLMLGICNGFQVLIKLGLVPYGEIRVPSESAPTLTYNNIGRHQA
                     KIARTRISSNKSPWLAQTNVGDIHNIAISHGEGKFVASEDVMRELIANGQVATQYVDF
                     NNEATYDIEFNPNGSFYAVEGITSADGRVFGKMGHSERIGEEVYKNIIGEKDQKIFES
                     GVKYFR"
     gene            32908..33780
                     /gene="rfbD"
                     /locus_tag="PC545_000362"
     CDS             32908..33780
                     /gene="rfbD"
                     /locus_tag="PC545_000362"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDF2455159.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSINQPKYVILATTRSDLDI
                     SNQTNVDNFILHNKPDVVVNCAAYTKVDACEDNIEIAYKINALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDNKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLAKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFKLKNIDIKVNPIKSNEFKCKAPRPLYSVLDNFMLKLIGLNSFRKWEE
                     SIEEYLERYDINEK"
     gene            33889..34758
                     /gene="rfbA"
                     /locus_tag="PC545_000363"
     CDS             33889..34758
                     /gene="rfbA"
                     /locus_tag="PC545_000363"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDF2455160.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLNITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLENAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYTKGYISREQLMKLAKPLIKTE
                     YGQYLLKLASEDM"
     gene            34807..35364
                     /gene="rfbC"
                     /locus_tag="PC545_000364"
     CDS             34807..35364
                     /gene="rfbC"
                     /locus_tag="PC545_000364"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDF2455161.1"
                     /translation="MGKINVIKTKIEGLYIVEPNVFYDERGYFMESYNKKDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVKYPQGKLVRVTEGEVFDVAVDLRKDSQTFGKWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFSYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICTRR"
     gene            35402..36385
                     /gene="rfbB"
                     /locus_tag="PC545_000365"
     CDS             35402..36385
                     /gene="rfbB"
                     /locus_tag="PC545_000365"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDF2455162.1"
                     /translation="MKKILITGGAGFIGSNFIHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVKGDISNREQVYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSSINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLEVVKMILNLLGKSEELISYVND
                     RPGHDLRYAIDATKIENELGWKAKYDFDLGIKETVKWYIENESWWKAVLSV"
     gene            36415..37083
                     /locus_tag="PC545_000366"
     CDS             36415..37083
                     /locus_tag="PC545_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2455163.1"
                     /translation="MNEISSLINILALTQLSGSNTTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNSYNLLSNSENLFNELDNVVNNTTSRFIDVDTKDKNVKLRIENAVDQA
                     SKKYNVDSNLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            37092..37520
                     /locus_tag="PC545_000367"
     CDS             37092..37520
                     /locus_tag="PC545_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HDF2455164.1"
                     /translation="MGINIDKLNISPSINMEVKKLKESNKFSDKLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            37544..37858
                     /locus_tag="PC545_000368"
     CDS             37544..37858
                     /locus_tag="PC545_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2455165.1"
                     /translation="MNSKKEDIYELNFGQLIEEKSGVIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLSKNVDEKLDIYANDRLFAYGESIITNERLSVRLSKIEDPD
                     VN"
     gene            38010..38285
                     /gene="flgM"
                     /locus_tag="PC545_000369"
     CDS             38010..38285
                     /gene="flgM"
                     /locus_tag="PC545_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HDF2455166.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGIKKVEEVKKINKTDKIELSKFAK
                     EMASVSNMSDENIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            38290..38706
                     /locus_tag="PC545_000370"
     CDS             38290..38706
                     /locus_tag="PC545_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HDF2455167.1"
                     /translation="MITDLKVIIYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSNDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPADKQTTTYNSYGNIRK"
     gene            38724..40034
                     /gene="flgK"
                     /locus_tag="PC545_000371"
     CDS             38724..40034
                     /gene="flgK"
                     /locus_tag="PC545_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDF2455168.1"
                     /translation="MAGLFSILNTANSGMNAHQKAIQTISHNISNLDTDGYSRQRTEF
                     ATNSPMYMPSLSNSIARGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKEDIDSNIDTEIKQINDMLKSLEDLNKSIDIISGSGSTPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNPDGTWKTGISGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVTDFFITSNVDGEDIIKINPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAESIGLDSQKVNQDEANQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            40053..40985
                     /gene="flgL"
                     /locus_tag="PC545_000372"
     CDS             40053..40985
                     /gene="flgL"
                     /locus_tag="PC545_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDF2455169.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMETYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKMKEIANALNATHEGKYIFSGSNTGVPPVECIDNADGSVSLKFN
                     SSLNLNKLNDSLSMDIAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGDMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKDILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFNVSILDYI"
     gene            41001..41393
                     /locus_tag="PC545_000373"
     CDS             41001..41393
                     /locus_tag="PC545_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HDF2455170.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDYLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVVLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            41387..41599
                     /gene="csrA"
                     /locus_tag="PC545_000374"
     CDS             41387..41599
                     /gene="csrA"
                     /locus_tag="PC545_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDF2455171.1"
                     /translation="MLVISRKKDEAVLIGESIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNIKILKSLK"
     gene            41621..42016
                     /gene="fliS"
                     /locus_tag="PC545_000375"
     CDS             41621..42016
                     /gene="fliS"
                     /locus_tag="PC545_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2455172.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIAKTAILD
                     KNTQKAHRELIRVQDIFTELMVTLDQNAGQWAKDMYRVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            42016..42372
                     /gene="fliS"
                     /locus_tag="PC545_000376"
     CDS             42016..42372
                     /gene="fliS"
                     /locus_tag="PC545_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDF2455173.1"
                     /translation="MELNLAELDKLSKEELLLMLVDGTVKYTNISKEALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNQLKI"
     gene            42393..43916
                     /gene="fliD"
                     /locus_tag="PC545_000377"
     CDS             42393..43916
                     /gene="fliD"
                     /locus_tag="PC545_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDF2455174.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNVIQESKDLYDKYLSVNSPNSIVSEKAYSSTRITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FINDAGGNKVANVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMQAFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKTLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            43919..44254
                     /gene="fliT"
                     /locus_tag="PC545_000378"
     CDS             43919..44254
                     /gene="fliT"
                     /locus_tag="PC545_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDF2455175.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISIKLGERQEIINSV
                     SEIDRNDFIQLYNRMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYNLNRE
                     KVNIFNKKV"
     gene            44356..45228
                     /locus_tag="PC545_000379"
     CDS             44356..45228
                     /locus_tag="PC545_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HDF2455176.1"
                     /translation="MRVNTNVSALIANNQMGRNVNAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTSN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNADGTSGTNAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            45321..47462
                     /locus_tag="PC545_000380"
     CDS             45321..47462
                     /locus_tag="PC545_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2455177.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRNSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFQELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLELAPGYLITSLVKTYLYLGKHKKCERLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLEKYEFVISEYNNIEDVEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKIDLLDIFYEMEYVWIEKYLKYLLALERSFNLVLYKYIISRPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLDIKNKVNNANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFKDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDIKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            47486..49363
                     /locus_tag="PC545_000381"
     CDS             47486..49363
                     /locus_tag="PC545_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2455178.1"
                     /translation="MNIPIVVNEKSKDIIKDWQYTCTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKNKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNNKNLGFPKGCNQGIE
                     LATGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSIE
                     DMFIFAHKINKSNINLWKYRLKLVGFCMLIKKTVLDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCQDTFIHHFGSVSFKENRNKYTDIMYINRRKFETKWGFNPEYSGMIRT
                     DLISKIDDSLEANINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIANIEKVKLDYKKDFFDYIILGDVLEHLIDPWKIVNELKQYLKKDGYLVSSIPNIMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITATSMPLSEID
                     NSFIDTICKESCKEMRQQYESYQYIVKASKILDTKRYSSISMINLKYALMRIDNELDV
                     DSNLSYIFDNYNITEPCFVDDIEYLIDTFVINKATLLNRLGVEAFNRGLGDFSINMFM
                     TVLEMDRDDIDSIYNITSVLCELEEFDTAYNLIKNSSEKVQSESDIQEILCFIEGKKY
                     E"
     gene            49356..51470
                     /locus_tag="PC545_000382"
     CDS             49356..51470
                     /locus_tag="PC545_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2455179.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIHDILEIFKKNTDVGMIGVAGVKDM
                     PVNGIWWDSDKKAGKVYDSHTGKMDIYEFSNFEECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIVPKQNKPWCMHDSGVVNINNGYDEYRIKFVKEYI
                     NLDLYRENFYEFDKKKASTILKSNKHVMDCMKEELTEQLRLKNYKDVLGIVSKIAHFS
                     VFNHTGLYSCEECERALLECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPDWLLKAINDSGGEVISLDLISDDYLKRAVALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGTSIADIVIDFSE
                     TSQLITLDKRGIDKSEILPMPLKEKEVFSKSEVRKRYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENLYWKTLSNVTDNRVICFGTTANIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDKFDYKNIKEVID
                     MIRRYNTSEFDRDKIKAKQSEIKHFVIDKHCIEIKNYIDKIYNEVNHHKVKYDLYKND
                     EIEDYQLFVALYGYMVKNKNMS"
     gene            51479..52474
                     /locus_tag="PC545_000383"
     CDS             51479..52474
                     /locus_tag="PC545_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HDF2455180.1"
                     /translation="MNNIHINKFEKSCEKNWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIICKGEEILAVVPACETYEDGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQNFDKVYLKNTSRIFCKEDTSLLEYFLFKNNYMFYDELSMFLELSKYKE
                     DILANFKYSKRRDFKYSLKNNLTFKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSVEEL
                     LEFKNNRLKDIVEFYAVYIDDIMICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLY
                     YNLIKDALERNFKYLSFGISTLNRGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
     gene            52566..53798
                     /locus_tag="PC545_000384"
     CDS             52566..53798
                     /locus_tag="PC545_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDF2455181.1"
                     /translation="MKRLMILGSIKYFENIVMSAKREGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKNIAIDKKIDGILTGFTDSLMKPYVYIANELNLPCVISCNQLESVT
                     NKAVMKNYFKNNGIPTTDYCIIESLKDLEKIQGLNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEASQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFGIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEVNFDEIKKYNMHLSKCVYALPIYIRPGTI
                     KKINNIDKIENLDYVTQFLLLVKEGDTIGLYEDERQDCGRVYGVANNVFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            53818..54762
                     /locus_tag="PC545_000385"
     CDS             53818..54762
                     /locus_tag="PC545_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HDF2455182.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVEE
                     GHVFYNVMPSLNHNHKIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFVKKYEKNEQFSFEICDTHEELIVDSDVVISTVTYTDEYLSEFKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQAKGRENDDERIISY
                     NIGLSIHDILFANKIYELCETLSCEEIELKEPKEKYWI"
     gene            54856..55263
                     /locus_tag="PC545_000386"
     CDS             54856..55263
                     /locus_tag="PC545_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HDF2455183.1"
                     /translation="MDKYNLIRFVSIGNEYIGNLVALESNKAIPFDIKRVYYIYDVPN
                     NVKRGFHAHKNLEQVLVCLNGSVKIKCYDGVKETIFNLHKNDVGLYIGTGVWHEMFDF
                     DEKTVLISIASEHYNEEDYIRDYKEFLKYLNNV"
     gene            55350..56441
                     /locus_tag="PC545_000387"
     CDS             55350..56441
                     /locus_tag="PC545_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HDF2455184.1"
                     /translation="MLVKFNNLLKQFQTHKEEYEQKIKDVMESGYYILGNEVKHFEEE
                     FANYVGSKYCVGLASGLDALIIAFRALGISKGDEVIVQSNTYIASVMGISINGATPVF
                     VEPNEYYNIDSSKIEEKINSKTKAILVVHLYGQASNMKDIQELCDKYNLYLIEDCAQS
                     HGAKYDGKMTGTFGDIGCFSFYPSKNLGAFGDAGAIVTSNEEIADKVKILRNYGSEKR
                     YYNKFVGYNSRLDEIQAGLLRVKLKYLNEIEIEKSKIVDRYLNEINNPKVILPKIENN
                     CTHVWHLFVLQVEKRDTFMEYLKKSGIESIIHYPIPPHLSEAYAYLGYKKGSFNIAEN
                     YSDKVVSIPLFIGMEDEEVSYIIKIINEY"
     regulatory      56750..56840
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            57248..57565
                     /gene="flgB"
                     /locus_tag="PC545_000388"
     CDS             57248..57565
                     /gene="flgB"
                     /locus_tag="PC545_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDF2455185.1"
                     /translation="MNTYDIIKMGLDATNLRSQTIANNIANINTEGYKRHYITFEETL
                     NDAMPKIEEKTDNSKGLRVDGNNVDIEEEKVNQAMATLQYNALISFANGKIAMTKSII
                     SGR"
     gene            57573..57977
                     /gene="flgC"
                     /locus_tag="PC545_000389"
     CDS             57573..57977
                     /gene="flgC"
                     /locus_tag="PC545_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDF2455186.1"
                     /translation="MSVFSGMRISASGLTAESLKMDIISSNVANINATRGPNGEAYRR
                     KVVSFEENYDNKLGMLGVKVTSVEDDKSPLKEVYDPSHPDAKPNGYVEYPNVNILVEM
                     TDLITASRAYDSNVDTLNAQKSMISKALEIGK"
     gene            57987..58304
                     /gene="fliE"
                     /locus_tag="PC545_000390"
     CDS             57987..58304
                     /gene="fliE"
                     /locus_tag="PC545_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDF2455187.1"
                     /translation="MPITQINNLMQGAKLDNLIGEAKPLTLNNKEEKKNFEDVIIDTI
                     DKVNFQQVTADDMKEKFIKGEDVSMHEVMLIGQEAQLSMQYLIEVRNKIHDAYQEMNK
                     VQI"
     gene            58329..59879
                     /gene="fliF"
                     /locus_tag="PC545_000391"
     CDS             58329..59879
                     /gene="fliF"
                     /locus_tag="PC545_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDF2455188.1"
                     /translation="MITSFKNKIKEQVAKSRTKISSLKKGTKIALGIGVIAICLALVF
                     TAISASKNKYAVLFSELDAQDAADISAELESKKIDTKVKGSTIYVPRTEVDKLRLELA
                     PSLTGGSKGFALMDDSSSIGLTDEEFQIKKQRMIQGEIEKTIKSFPQVEDTRVHITFG
                     ESSVFEKEAVPGSAAVYLMLKPGTTLEKNQIKSVMALISGSVTNIPEKNVQVIDNKMN
                     LLSEGILEDENVDENNQPINSGISVDKNKASEQALNKQLEKSILEMLEPIFGKGKVKA
                     TVNADLSFDTAEKTEIKIDPDKVAIKETRSKNSSKSAANNVQGVDANMNNQGNNNGTN
                     TEDSLDESFEYETGKIETHTIVAPGELNRLTASVAIDGKIAKGVQADVEDIVNNAIGM
                     DGARGDTLSVVSMVFDPEGKKEAKQELEEIKKEGFKKNIIKAVIGVVAVIAVGAIGYL
                     IYKKRSEREDMDYEDEDYISKSDSVDRKDNPLLGDDPELTLEEAVKMYAEEKPDQVTE
                     IIKTWLNE"
     gene            59892..60962
                     /gene="fliG"
                     /locus_tag="PC545_000392"
     CDS             59892..60962
                     /gene="fliG"
                     /locus_tag="PC545_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDF2455189.1"
                     /translation="MSTEITNVNAFDLSDINESDKNEKVEGEGLKKAAVLLMTLGPNV
                     SSDIIKSLPDKQVQKIGLEIANISSIASKERREILKEFVEINRAKDYVIEGGLEYARE
                     LLSNALGNVKANKLLEGISINTSTKPFKHIIKLDVKKILEILRNESAQTIAVLLAHME
                     PENGAEVLSYLDFDLQNEVAMRISSMGSISPEVIKVIESSLEDKLHSISGDDFNDYGG
                     VSTLVQILINSDRKTEKNILGTIEEQNPDLAAQIKSHIFVFEDIVKLDDISIQKVLKE
                     VTMKDLVYALKGVDDEISDAIYRNQSSRAADALKEEMEMVGVIKVTQVEASQQKIANI
                     VRKLEREGAITIVRNFGGDFVE"
     gene            60955..61686
                     /locus_tag="PC545_000393"
     CDS             60955..61686
                     /locus_tag="PC545_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDF2455190.1"
                     /translation="MNNKIIKSKKALYKGVLDLTIIDKVRVGESNVNKENDCRFRDEV
                     IKEAIESAEIKKNEILEEAKINAQNIEKQAYEKGYSQGQKNGYEDGYKESYDEYTQKA
                     KDEADFIKNQANLILLQANEKMTEYIKEKNKEIVRLAINMASSVLKKHFEDEESMSDL
                     LKQIIQDYEGSSSFIVRCNSFYKDNIEKEFELFKEETSLKSSVFVIADDSIDKGNAII
                     QKENGRIIVGIDCAMEKLKEELLGE"
     gene            61690..63009
                     /gene="fliI"
                     /locus_tag="PC545_000394"
     CDS             61690..63009
                     /gene="fliI"
                     /locus_tag="PC545_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HDF2455191.1"
                     /translation="MYKTDISIDFDKINKKIENLELTISEGRVKKIIGLTVEVEGIKA
                     FVGELCVIYNQVNKPVNCEVVGFKDKEVILMALGELTLIAPGCKVISKGIPLSVMCSD
                     NLLGKVLDGLGNPIDNSDAVLGDRYNLNNEPPDPMKRKKIRNIMETGVRAIDAFTTCG
                     EGQRIGIFAGSGVGKSTTLGMIARNAKADVNVIALIGERGREVLDFIDKDLGEEGMKK
                     SVVVCATSDKAPLVRLKGALTATAIAEYFRDQGKKVILMMDSVTRFAMAQREVGVAIG
                     EPPAQKGYTPSVFAMLPKLMERTGTSDKGSITAFYTVLVDGDDFNEPIADTTRGILDG
                     HIVLSRDLANKNHYPSIDVLNSLSRLMNEIASKEDIKIASFARDMLAEYREAEDLINI
                     GAYASGTNKKIDEAIYYHEHIINFLKQGINEKSSFNETISSLRRVFE"
     gene            63040..63486
                     /gene="fliJ"
                     /locus_tag="PC545_000395"
     CDS             63040..63486
                     /gene="fliJ"
                     /locus_tag="PC545_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HDF2455192.1"
                     /translation="MELDKNFKFRLQKVLDLKVKDEEEIKMEFAKIQQKKIDIETNLE
                     NLESNYSKYSISKNNDSIQNQKITINYLLALNNSIMDLSEELDKSTNELEKARKQLIS
                     KQIERKSLEKLKEKKYGQYYKEENLKEQNTNDEFASMSYLRNRQVL"
     gene            63515..64720
                     /locus_tag="PC545_000396"
     CDS             63515..64720
                     /locus_tag="PC545_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDF2455193.1"
                     /translation="MKIQADSYNIIKDIGDRKSSSKNKNIKNNKFEDNLYKATDHKKV
                     KDRREVSKQNISSDKPENSDNECHLDEDKSNHLEDENTYEKDCSKNNEALQQLLNLLK
                     DNYKKDKDINTILNNIEKLSISDELKQELKLNKNILNKIANQNSELNKANINNSNDIE
                     NLDIVKLRELLEDNANKDIEVITKTTENDTKNIDLYSFDSNRMDNLNSNKQKDDSSDI
                     LEKLAGVNGNKSGNFGQVINKNVDMNKIKNDNVNLKQIESNVMDDSIKAIKHMKTNDI
                     QELTIKLRPKELGDMNIQLLKDGESMRAVVTVFNKDVFDSINKNIADLKQHLELTNVN
                     IKDVSVQMHSDNTNTSDTFDKAFEQQNRQNNQENMSKKNTNDKHNNVVIEDDIKENTI
                     DDDRVDLLA"
     gene            64738..65346
                     /locus_tag="PC545_000397"
     CDS             64738..65346
                     /locus_tag="PC545_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgD"
                     /protein_id="HDF2455194.1"
                     /translation="MYGIATALAGTQGNYRSNNLSQLERNINDIANKQVRSKGNQTVT
                     DNGTPIIGQGDDADKDLFMKILVAQMGNQDPMNPQDPTEYVSQLAQFATLEKMSAMNE
                     KLDVLLALSNSTLINSALSMATSLIGKEVEVSGEEKEDENIVGVVKSTYIKDGEVHLE
                     LEVDGSDKTVGVKYEKLLKIGEVDENSGEENDSSEHESPEEA"
     gene            65373..66356
                     /locus_tag="PC545_000398"
     CDS             65373..66356
                     /locus_tag="PC545_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HDF2455195.1"
                     /translation="MIKAMYSGVSGMKANQTKLDVIGNNVANAGTTSFKKSSARITDS
                     FYQTVLYASAPTAALGGTNLGQVGVGSKISSINKDMVQGNVQPTNRPSDLMIDGDGYL
                     PVVRQGTVMYTRDGSFNLDTGFKAADGALKNIKGGRLVNGDGYMLQGMIYDGEYKADT
                     GEFVKKGDAKGKEPIVIPLQSISPKDGTVQNVLEYNISKDGTVEFLLSDGQRTQFVYD
                     IPDGAKVGDTPAGIDKLKGTIQKVQTYAFQNPAGLDAEGGNLFKPSANSGDPQIAGAT
                     GTIIQGAIEASNVDIAEEFTEMIIASRSFQANSKIISTSDEILQEIINLKR"
     gene            66369..66572
                     /locus_tag="PC545_000399"
     CDS             66369..66572
                     /locus_tag="PC545_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HDF2455196.1"
                     /translation="MIRVTDLNGRKYILNSDLIVRIDSVPESRILLTTGEKNLVKESV
                     DEIVDKVIKYKRKINVGYIRSEK"
     gene            66572..67390
                     /locus_tag="PC545_000400"
     CDS             66572..67390
                     /locus_tag="PC545_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HDF2455197.1"
                     /translation="MKKTDSLTFIGLIVSTVLVLVGAAKGSSSGLKNFFDVSSILITV
                     LGSFGALMITFTIDDIKLIKNALQYSFKTMSVSKLDLLEQFKTLSKKARKEGLLSIES
                     DVMGIEDPFMKKGLELCIDGLEINEIRSILELEIDSVEDTNNRYSKLFKTWGTFAPAF
                     GMLGTLIGLIQMLADLTSPDLIASGMGKALITTFYGSLLANIALNPIAYNIDEKTEKE
                     IYVKEMMLEGIISIQSGESSIVVEERLATYLSNNEKLEIMKSNKNTERAMSNGA"
     gene            67380..68075
                     /locus_tag="PC545_000401"
     CDS             67380..68075
                     /locus_tag="PC545_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2455198.1"
                     /translation="MARKKNKKKDEINPDAWLATYADTITLILTFFVLLYATSVVDME
                     KFKNIADALKGQFSGTSIFSTGSSTSEKSPIDSVLVDKNDKSNLEQDLKDKVNNSNLQ
                     NSVTVKDDSRGILLELDDSILFDSGISELKVDSKATLNKVYDMIKMMKNKIVIEGHTD
                     NVPVKSSTHESNWDLSSSRAVSVVRYFVEEKGMDPRLFSATGCGEYQPLVSNETAKGR
                     AENRRVNILITID"
     gene            68087..68530
                     /locus_tag="PC545_000402"
     CDS             68087..68530
                     /locus_tag="PC545_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDF2455199.1"
                     /translation="MFKNKKIIIIILILIIFSAGAGFFILGEGGNKQVDKIADLFIPT
                     GKGKDEVKTENLDLEEMVLKLADEGVRYLKVSITVSYDSSYKDIEKNTPLIRDNIINC
                     FMAKRADDFTADNLKNIKEEIKNTLNIQLGENVIQNIYFTNIVVQ"
     gene            68545..68919
                     /locus_tag="PC545_000403"
     CDS             68545..68919
                     /locus_tag="PC545_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliZ"
                     /protein_id="HDF2455200.1"
                     /translation="MILYLLKILICIPIILILIVLSLKISQGTLLKKYNDKYVKILDI
                     VNISKNNSIIILKIGEEGCIMASSSSSMSKIKDLTKEEILKIENDIKERNFKIQDFKM
                     ERIKKKYDFSRYIGKFTKKEDK"
     gene            68920..69581
                     /gene="fliP"
                     /locus_tag="PC545_000404"
                     /pseudo
     CDS             68920..69581
                     /gene="fliP"
                     /locus_tag="PC545_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425215.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            69594..69863
                     /gene="fliQ"
                     /locus_tag="PC545_000405"
     CDS             69594..69863
                     /gene="fliQ"
                     /locus_tag="PC545_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDF2455201.1"
                     /translation="MSEFMIMSVLKNGMFTGAMIAAPVLIVSLVVGLLISVFQATTQI
                     QEQTLTFVPKLIAILLVVAIGGSWMLHTLISFTSEIFNMISLITK"
     gene            69875..71689
                     /locus_tag="PC545_000406"
     CDS             69875..71689
                     /locus_tag="PC545_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused FliR family export protein/FlhB family
                     type III secretion system protein"
                     /protein_id="HDF2455202.1"
                     /translation="MIFVFIRIVAFFGTLRILFPAGTPALFKSLFAIIISILISSTMQ
                     IEYATNIDNIVLFTLYGVNETITGIMLGYITNLCFYSIRMAGSMMDQQMGLSMINMFD
                     PNSMTQTTLIDNLMNWTALMIFFSMDGHHVLIRGIRYSFELIPIGKPFVDNNIDYIIN
                     IFVQCFLTGFKIAIPIVLCLLMADFILGLISRSVPQLNVMIVGMPLKILVGIALFIIS
                     IPLIVNQISHLLSQIPKMYEGTFALAPMFFMGSADKTEEATPKKKGEQRKKGNIAKSR
                     ELPVAMTLLAFTLLVPTLFSYVVDTLKSSLNYFLSLDFYMNINYSNLEKLVIAGLMDF
                     FKIFLPIAIPFLVLGIIANLFQVGILFTGETLKPNLSKLNPISGFKNMFSMRSLSTLI
                     KDTAIISILAYIGYTFFQDNYLDILKLGNIYLPTLMYTVKDLVYSILSKICVAMIAIA
                     VADYVYQRYSHKKQLRMTKQEVKDEYKNSEGDPEVKAKIKQKQRQISSQRTMQAVPSA
                     TVIVTNPTHLSIAVRYEKGKDQAPVVVAKGADYLAFKIREIAKGNNIPIIENKPIARL
                     LYKQVEIDQEIPEDMYQAFAEILVAVYKIKNRYKVPKR"
     gene            71698..73773
                     /gene="flhA"
                     /locus_tag="PC545_000407"
     CDS             71698..73773
                     /gene="flhA"
                     /locus_tag="PC545_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDF2455203.1"
                     /translation="MNKFNLKENMDVIVAFGVVGIVLMMIVPLPTFILDILLAINISL
                     SVLILLMTLFTTNVLDLSIFPTVLLVTTLFRLGLNLSSTRLILTQGYAGEVIEAFGSF
                     VVGGNYVVGIIIFLIILIVQFVVITNGSGRVSEVTARFTLDAMPGKQMSIDADLNAGV
                     IDDKTARKRRKELQQEADFYGSMDGANKFVKGDAIAGLIVTAINIIGGIVIGAVMLNM
                     TLMGAAQTYVRLTIGDGLVSQIPALLISTSAGIIVTRASTDENFGDLVGKQLTAIPKA
                     VMMTGCVLIVLGMMPGLPKPAFFSLGIGAIAAGYFLGREKKDTLEDLEFEKPDMVNPD
                     KNEVEDVTNLINVESIEVEIGYGLISLADESAGGDLLQRIASIRRQCAIDMGIVVQPI
                     RIRDNLQLNPNQYNIKIKGNVVGGYEIMPNMVLCMDPMNQGFSIFGIKTIEPTFGIEA
                     LWIESDKVEDAELKGYTVVDSSTILITHLLDIIKSNAHELLGRQEVKTIIDAARENYS
                     AVVDELIPDLMTLGEIQKVLKNLLREKVNIKDRVTILETLADQSRNTKDIELLTEYVR
                     IALARSICNNLVDDDKTIVVATLSPETEELVSNNLQRSVNGTYPAVDPENTNKIFESI
                     QEVMSNVYFNHNIPVIVVSPKIRAPFRKLVEIVYPNLTVLSLNEIPNDIKIKAEGVVS
                     IYDDEKVYS"
     gene            73748..74764
                     /gene="flhF"
                     /locus_tag="PC545_000408"
     CDS             73748..74764
                     /gene="flhF"
                     /locus_tag="PC545_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HDF2455204.1"
                     /translation="MMTKKYTANTIQDAMNLAKMELGDNITLIDKKEVRKSGIQGIFS
                     KKDIELTIGWEKRENVEQKDLKREIEQLKSIINNMGFDNKNDNDIDKICKNLLSLELN
                     EEIVESIRIDLQEMKFNGIDTSKNLVEILKKKIKIENQVINGKIALVGPPGVGKTTTI
                     AKLAAKLVFEENKKVGVITIDTYRIGAVEQLKIYTDIMNIPFKGVISPDEMELALDEM
                     KDCDVVLIDTTGRGYKNSMQILEIKNLIDKAETDNIHLVLNCTTRESDTKAIIDSYRN
                     VNFKSLIITKLDETITYGSIFNIMNYAQKPISYITTGQNVPDDIIKPNEDKIIRLLLG
                     VESI"
     gene            74761..75639
                     /locus_tag="PC545_000409"
     CDS             74761..75639
                     /locus_tag="PC545_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HDF2455205.1"
                     /translation="MIDQAQILRKIAMEKRGLDEFIVENENVPKIITIASGKGGVGKS
                     NLATNLSICLTKLNKKVLILDADIGMSNIDIIMGVNVKGTIIDVINGEKNIEDIISQT
                     KYGVNIISGGSALNHIEDFTEAQRNKFIHSIEQIHDVDFIIIDTGAGMSKSLLSFIYC
                     STEFFLITTPEPTSLTDAYSLLKAISNFGIKRSANIIINRVIDLEEAKSTYKRIKMVV
                     DKFLNLNLELYGYIIDDKKVSLCVKKQIPFILEYPTSIASKCIIAIAKRMVSQSREDI
                     VDNKSFLRKMFSIFKS"
     gene            75653..76354
                     /locus_tag="PC545_000410"
     CDS             75653..76354
                     /locus_tag="PC545_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDF2455206.1"
                     /translation="MNREELIKENMPLVKSIARKFFIPGKGFEYQDLVNSGVIGLIDA
                     INKFDAEKGAKFSSYSYIKIKSAILDEIRNQSPISKHNLTKVNKYNRVVEKLQSKLLR
                     EPTSYEIAKELKVSEKELHDIESNIDMLNIVSLNYVIFEDTNETVQDVISDREEEAPE
                     NIIEEEEKLEILSKAISNLNEREKLILSLYYYEDLNLKEIGKVLGVSESRVSQLHRKS
                     IRNLRNKIKELKYSI"
     gene            76370..76792
                     /locus_tag="PC545_000411"
     CDS             76370..76792
                     /locus_tag="PC545_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2455207.1"
                     /translation="MIQIILIVVSILIIVTIVINLHKESKNKDNRYFDKIFEQEYEAN
                     DRLIIAEKRRFFLDKVLAEKRINSHIKVYENYNLIKANEERKENIFIESKKEVRQENE
                     LSLKVKHLISSGYDDVEICKMLDIGKGELLLIRSCYKI"
     gene            76777..76962
                     /locus_tag="PC545_000412"
     CDS             76777..76962
                     /locus_tag="PC545_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HDF2455208.1"
                     /translation="MLQNIRILLCDKKVFLGIGIGILISTLCIMLFSNNDMSKAEIEK
                     KARALGMKYTSEMKVLE"
     gene            77003..77776
                     /gene="flgG"
                     /locus_tag="PC545_000413"
     CDS             77003..77776
                     /gene="flgG"
                     /locus_tag="PC545_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2455209.1"
                     /translation="MIRGLYTAVSAMITNQQKQNVVVNNLANMDTNGYKSKQLLTKSF
                     DELKLEGYNNYANGTKQKQIIGDISPGISMDETITNFNQGPIKTTDNKMDVAIQGKGF
                     FLVSDAAGNQFYTRNGNFRTDNQGDLITSEGYYVLGTNTATGAVGHINVGNQKFEVAK
                     DNTISLDGNAMYKFNIVDFNNYNNLKKEGDNLYSGGGAMKVNNALTIQSAIESSNVDM
                     VSEINNMMTISREYEANQKIIQAMDSKLAKIASEIGSVR"
     gene            77797..78558
                     /gene="flgG"
                     /locus_tag="PC545_000414"
     CDS             77797..78558
                     /gene="flgG"
                     /locus_tag="PC545_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgG"
                     /protein_id="HDF2455210.1"
                     /translation="MYTMFYTSKTALSANQSKLDIISNNIANSDSTGYKKINMEYSDL
                     VDEVLNRPSYPTNGNDISTGTGAKATAPMRNYEQGALAPTDSKSNLAIDGEGFFRVIR
                     KDGTYAYTRNGGFNVDALGKIVDDNGNILDVQFDAGYNYNNTNIDSTNLTVSRNGELF
                     VDNKKIGKINLYQPIGTQNFISEGDSLFVATDAAQIKQVEKVDIVQGYRERSNVSLQE
                     EFVDLIATQRAFQMNSKGIKTADEMWQIANNLRAK"
     gene            78581..79462
                     /locus_tag="PC545_000415"
     CDS             78581..79462
                     /locus_tag="PC545_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2455211.1"
                     /translation="MNDMKEYDFGKPQSYSRENLTCLNFLLAEFCKKYKNYIIYELKC
                     NSSMVVDSIDQVNYQTFLDRVTSSCVVVQNAMEPVMKNFSFRIDRSVADMWIDITSGG
                     TGVVKDNDRELTELDKKVLLHLMDDLVRNMHLFEGFENIQVLDTHTHINLPQLCSPTA
                     PVCVVDLKVLNDNEYIGSVSLCFPYNGLEALLESISVIEFFDNDVGDDSEEFTQKIYN
                     SVSNIELSVIAEIGKVSINVEDLLKLEVGDVIVTNKKINDYIDIFVENSKSYTATPGF
                     ISSKKGVKINDAVGKEV"
     gene            79465..79839
                     /locus_tag="PC545_000416"
     CDS             79465..79839
                     /locus_tag="PC545_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HDF2455212.1"
                     /translation="MDNSNLISDEEAKMLLNDKVESEVDGDDELALDVSEVNRNNIQR
                     ILDLKLELSVVIGRTKMTLRDILNLHKGSLIELDTLADQDVEILIDNKVLAYGKVVVV
                     DLNFGVKITSIVSEEDMVKSLV"
     gene            79871..81274
                     /locus_tag="PC545_000417"
     CDS             79871..81274
                     /locus_tag="PC545_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2455213.1"
                     /translation="MVLILNLNDIKKDILKLNKKDSCSLYNESINNINNNCISTGLKF
                     LKQARDLNPDDVDILNLTGLVNLLKCNFDDAVESFYKSSLCEKTALCEKYINMLTSEE
                     FLIFFEKYNQLIRLINKGMYKEAIQGFKLITDQYCDLIEPYELLALLYDKEEEYIKFD
                     ECLEVLKIIDKENYLLNQENNTLYMYNSANIQTKSNIKVDDYKNEFLHKSSSNHKIND
                     YNDVTKEKSSSKNQKSSDSNKKKSIKKNKYLYIAIILGILLVGQSIYSSYKMNNLTDK
                     ISKNNETKNEESIDSNLTKKENNIEENEDMSKASNINKSNKNINKEDDVKSKKNTVEN
                     EDDKDSDLFTEDELMSKANTLKSEKKTKSSIQLYKKVADIGENKGNTSEATYQVAILS
                     EKLKDYKTAEEYYKMYVENYSEKDAYFDESYYNLGMMYYNNGDLKNSKLTLKKLVNKV
                     PNSMYNNSKVKEILKEE"
     gene            81390..83327
                     /gene="htpG"
                     /locus_tag="PC545_000418"
     CDS             81390..83327
                     /gene="htpG"
                     /locus_tag="PC545_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDF2455214.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKITVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDARNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEIVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFNVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKEAVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKITLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            83714..84844
                     /locus_tag="PC545_000419"
     CDS             83714..84844
                     /locus_tag="PC545_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2455215.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKSTGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKASIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPLADVQAIYG
                     IELISENLIKVYNDVNDLEAWEKITLASTIGGMTIGTAGTALPHGMEHPVSGLRDVMH
                     GKGLAALTPTIIESSWDSDIERFGNISKVLGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEQIKEMYHKSL"
BASE COUNT        34188 a         8981 c        15168 g        26585 t
ORIGIN      
        1 atccccctaa aaaactttca taaaaacaaa agaacaatat ctctttggca actggctgac
       61 ataactcata agatatttat ataagtctta gtccctatag ctttgcgtca ctaaatttct
      121 ctagttttgc cgatttagtt ttattctaca actaaaataa tacaatagaa ttagttatta
      181 ttcaacaaaa ttgtttgaaa atttaataat tttatagata tgaaagttat tttcttctaa
      241 gaaaacagac taaatgcgta gaatatttgg taaaacttac ctgtataaat tgaaatcaga
      301 aggttttatt attgatttag attagaacaa tttgagggag tgacataaaa tgaaacaaca
      361 agaagtagtt aataaactac gcaggggaga attgatgttg atggaatact tgtggaggaa
      421 aaattctatt ttatctaaaa aagaaataat agaagcaatg aaagaaaaat ataagtggag
      481 gaaaagtact acagaaattc tattaaaaag attagtaaaa atgaaaacat taaaaaagaa
      541 gaggataggt tttcagttta gttatgaagt gctagtaact aaaaaagaat atttaaatgc
      601 aataaaagaa gatagaaagg taactaaata tgatagtttt tttatacgaa tgtttaccac
      661 tatgcataaa aaagaaaaga tgacagaaga acaaataaaa aaatatatag aaaatatgga
      721 aaaaattgga aaagaataat ttatatatat attaattatt tttaaaataa atttgttata
      781 atatttaaat atacataaac atacataaat ttacaaaaag ggggtgtagc ttatgggatt
      841 caagaaattg ccagattctg agttaaaagt aatgaagttt atttggggat tagatggaaa
      901 taaggtagca tcaggagaag ttgtggaagc aatgaaacaa atttatgact ggaataaaaa
      961 tacgacatta acaatattgt ctaagcttgc aaagaaaaac tatttgtatt ctcaaaaaac
     1021 aagcaagtgt acatattata ctatcgctgt aagagaaaaa gattatttaa aagttgaaac
     1081 aaaaaaattc tttagttttt ttcataataa ttctttaaaa agttttttca ctgctttaaa
     1141 tgatgaagaa aatttaagtg atgataaatt agatatgtta gaggaatggg tgaaaaattg
     1201 ggaagaagat gaataaaatg aatgtgatac taaaatgata ctaatatgtt ttatagcgtg
     1261 ttaaaaaata tagaaaatat agaaataata gaagaaaatt aatgtaatag ataaggtttt
     1321 atcagattaa aaagaaaatc aatagaatgg gaataaaata tagaatgcca aaaatccaca
     1381 taaatacagg atttgaggtc tctcttactt ctatgttata ctggtttgat actatcaata
     1441 tttgccagta tcttatggat taataagcaa aaaacatcct gctgaatgat aaattcagcg
     1501 gggtgttttt attttgtttt agaactcctt tgctctgaaa ctcgaagtat ttaacactga
     1561 tgattttgtt atcttttagt tccttttgtg tcatgctctg aaagacaaat tgatacaata
     1621 aaaaagtaat ttcttaatca ttgatggaga tatgtgttta tgttactctt gatggtaatt
     1681 tgagtaatat tgttcatagc tttttatatt cacttaatta gaaaaccatt tggtataatc
     1741 ataaataaac ttgatgatat aatagaaaag atttataatt attagaagta tataaaagta
     1801 ttttatgtct atgaaatgta tgtctataaa acatatagat gacttttgta tattaatgta
     1861 tgaatattga tattatagaa gaattgagtt gtcaatgata ctaaatcaaa tatttataga
     1921 agctggaggg attatgagaa ataaaataat tataggaatt actttttcac ttgtattact
     1981 aatgtctgga tgtgcttcaa aacaaaccgc agaaataaac gtattagcaa ctactgattt
     2041 acatggagtt ataccttatg agttaacttc ttatgtaaaa gaagaaagaa aaaaagataa
     2101 aaatctaact ttagtagatg caggagattt ttttgatagt ggagaagtgt ttggttctag
     2161 catggatagt tattttagtg ctagatataa agatggtgat aataacactg aaagttatat
     2221 tgagacgcct atttcaaaag atatgaaaga ggtaggttat gatgttgcag ttcttgggaa
     2281 tcatgaattt atatccaaca ataaatttta tctagataat atgatatctg attttgagaa
     2341 gcaaaaaata aatattttat cggcaaatac gtataaaaaa aatggagata gttatacaaa
     2401 accatacata ataaaaaata ttaatacacc tgaaggaaac gtaaaactag gaattttagg
     2461 gcttactata aaagaagttg gtgagaggaa acagtggaaa gatggaaaac ttgttgactc
     2521 taagtcttta gaactaaaag accaagaggg ttacaatggt gaattgtata tgaatgattt
     2581 agtagaagat gctaaaaagt gggttggtat aatggaaaaa gagaagcctg acattatagt
     2641 tgctgtggca catacaggtg agaaacctaa gaaacctaaa aacccaggaa atagaataca
     2701 agacttagct caacaagttg atggaattga tgctatagtt gcaggtcata atcatgttca
     2761 aattgagcaa catgattata aaaataaatc aggagaaaat gttatagtta ctgagccagg
     2821 aaagcatggg gaatgtattt ctaagattaa ttttaaatta gaaaaaaaca atgataaatg
     2881 gaatatagtt gacaaatcta gtaaattaat taaatttgaa aaaaatcaag aaagtgataa
     2941 atttggagaa tttatttctt caattaataa agtaagtaaa gataagaaag aaataagatt
     3001 atcagaagta aagactcctg aatgggataa aatatatgtt tttaaatctg gaacatctgc
     3061 agatgatata tataaaacaa taggatataa atggagaaat atatcatctc caaagggtga
     3121 tgacatggtg cagattgttg taatgaataa taataagcct gttagctatg tatatggtga
     3181 agctgaagtt atgccaatta atataaaatg tgatgaatca gagtataaag ataatgttgt
     3241 aactatttat cctaataaaa atgataaatt taaaattgaa aaaggtaaac aagaatatag
     3301 tattaattta aaacatatac aatagaaact tttatcttat ttaagtttag attgtactta
     3361 aaataaagtg tgtattattt taaaaattga tgataattaa aatcacaaca ttaaagaata
     3421 gtacacactt tttatgctaa aaaatagaat gttaaaaaat taaaccttta aattaaagta
     3481 cattgcttat attatccaat attattttca aaggttgtgt aatattttta tccttatgta
     3541 atattgctaa cattttattt tccatatcta tcccatctaa ttcaatcaat cgcaattttt
     3601 tatgtaagag tgaatctaat aataaatttt ctggcaaaat ggttatacct aaccctgctt
     3661 gtgcagcttt aataagtgca aaagaattaa cactttccca aacaggataa gctttttggt
     3721 ttgaaagaga taatacactg tccaaagtat tgcgaatggc acttccttgc tcacgtaaca
     3781 ataacggaaa cgtgcataac tgtcgtagag ataaggcttg tttagtattg gtggtggtta
     3841 tgcaatcata cttttaatcc aagagaaagt agtaaatcaa actggatgga taagtgagaa
     3901 aatgtttaca gatattataa ctatttcaca aatgacacca gggccactgg ctgttaatac
     3961 ttctactttt gttggacttc aaattggagg gttttgggga gcagttgcag ccactttagg
     4021 ttgtgtatta tgtggtgtag taatatctct atttttatat aatttttttc aaaagcatca
     4081 gaactctact tatatatttg aaatattaaa tggtctaaaa tcagcttcat taggtttaat
     4141 tatctcagct gcggctacaa ttataatcct tgcattattt gggacgaata aagtgtactt
     4201 caattctata tttgagatgc taaactggac tgcactaata atcttttttg ttacactact
     4261 gatagtacgt aagtggaaaa ttaatcctat tgtgattata ataataagtg gtatactggg
     4321 aatatttttt tatttataag tttttggtaa gtagaggata tctatttttt tatatcttaa
     4381 atccaaatag ccaataaagt aaggccattt ggatttttaa taaattctga aaagataatc
     4441 agaaacccat ataacattta aattatatca ctggttttct acaaaccaat cataagcttc
     4501 ttggcaaaat ttctcactat ctatatagta actattatga acattattaa tttcaacaag
     4561 tttcttttta cattttagtg aattataata gctatcaaaa tattgggttt taaaatatct
     4621 atcatgaagt gttttaaaga caagagtagg tatgttgact ccatctttta tcttactatc
     4681 ttttgcaata aagcattcta acattgtatt aattatcttt gtactatttt tccaaatatg
     4741 aggatttttc ttttcaaata ttttataaaa ttcattatca tagacttcat tataaccttt
     4801 atatttaggt ggttttactg ttatgtgaaa attaggaaga aacttaaaga aaaagtttaa
     4861 taatttaaca gtgagatagg tatagcctac tcctgttgtg tttttataat caacacaagc
     4921 aaactgagca aaacttttta acttattgtc tgaacatgca taatattgtg caaacatacc
     4981 accaattccc gtattcccat atagatgtat gttggaagaa taattttttt ctatgtatgt
     5041 aatgacagaa tctaagtctt ttacaattga attgcgagaa aaatcttttt catatccatc
     5101 acttttccct gttcctgaaa aatcaaaagc aaatatattt acattatgtt tggcaagtgg
     5161 ttcatatgct ttttgcatct caagcgtagt gacaatagga gtatgtgtaa caacaatatt
     5221 catggatgat tttctggtaa taaatgagtt acaaaaaatc attgtatctc catttggtat
     5281 ttttatatgt ttccaactca tttgattttc ctttctctat tataaaatat aataattttt
     5341 atgttctgtt ttatatatta ttcatatttt atcacagata atatactaaa gttaattttt
     5401 ttattgtaca cctattataa taattcttct ataatataag acattctata attttatatt
     5461 taagttcatg tgtatttaat tgtttttata taaaatgtcc agtaatacta tttggattta
     5521 tttttatttc atcaggagtt ccagaagcaa caatttcccc tccatttatt ccactgtctg
     5581 gacctatatc gataatccaa tcagaagcac gtataacctc tgtattgtgt tcaataatta
     5641 aaagtgaatg ctgttcattt acaagttttc taaaagtaat taacaaattt tctatatctt
     5701 taaaatgaag tccagtagta ggctcatcca atatgtacag cattcgtttt ttagtttttg
     5761 tccctaattc ttttgctaat tttatccttt gagattctcc tccacttaat cctaatgtat
     5821 tttgtccaag ctttagatat ccaagtccca cttcatctaa taaggagagt tttctatata
     5881 tatcagagca atcgctaaaa aatgtttttg cttctgctac ttccatgtca agtatatctg
     5941 caattgtctt tcccttgtat tgtatagaca gtatttcttt ttgatatctc ttaccttgac
     6001 attcactaca agttacccat tggtctgcca tatattgcag aataattttt gttttaccta
     6061 atccttgaca gtttgggcat tgcccctttt tgctattaaa gctaaaatag ctttcatcca
     6121 ataatttttg ttttgcttgt ggagtttcag caaatagaga acgaatttca tcaaaaatat
     6181 tgatatatgt agcaggtgta gaacggctag attttccaat aggtgtttgg tccattaaga
     6241 taaaatcatc aatagcatca aatccagtaa attcagtata atttttatct gggatagatt
     6301 tttgtttcaa tttttcttct agagcaggaa gtaaactgtg aaatacaaga gagctcttac
     6361 cagaaccact aacacctgta atagagcaca tacagtttaa gggaatttct acatcaatat
     6421 tttttaagtt atttgcatgg catcctttta agcttaacca tttacttggt ataattttat
     6481 tttgtgaact tattttattg tatgtgctta aatatttacc tgttatagag tttggatttt
     6541 ttataatttc ttgggttgta ccttctgcaa taatgaaccc cccttttgta ccagcacttg
     6601 ggcctacatc aataatataa tcagcagaca gtattgtatc tttatcgtgt tccacaacta
     6661 taacagtatt acccttatct cttagctctt ctatcatttt gatgattaaa ttatggtctc
     6721 ttgggtgtaa accaatagat ggttcatcta aaatgtaggt taagcctact aattcacttc
     6781 ctagctgact tgataatcgt actctctgca gttctcctcc agataaagtt ggagctgttc
     6841 tatctgttga tatatagcta agacctacct ttaatagata actaactcga atttttaatt
     6901 ctgttaagat atcatttacc aaagagagct catttttttg aagttgattt ggtaattcac
     6961 acaaccaatt ccataactgt tctatcgtca tttttgttag ttcagggaag cgataacctt
     7021 tgattgtagt gaatctagcc tcaatgcaca gcaattcgcc accacatgta ttgcaaggaa
     7081 ttttgttcat atattgatgc aaggtattat tttcagaact agattctcta aaaagtcttt
     7141 gaatgtggtt gattgctcct gaagcaggtc ttctaatctt tgattctcgt ccctttgatt
     7201 caaagctaaa ttcatatatc ttatcgtctg taccatataa gattgcatct ataaaatctc
     7261 ttggtaattc attccaaggt atatctaggt ctatattttt atcttttgct attgcatata
     7321 attcacctac catccaatta ccatttggct ttttcccctt caattttcca aaatagtaag
     7381 tagctccatc taatattgaa atagttgggt cttttacaat caattgagga ttaacttgat
     7441 aggtaaaacc taatccatta caatcgtgac aagctccagt atgggagtta gcattgaagg
     7501 ttgaagtatt tatcttagaa aaaatagttt gacaatgtgg acaataattt tttgtattat
     7561 tctttttttc taatggaatt ttacaatatg gacagtaact ttttccggtt gatacaaata
     7621 acattcttaa ataatcccaa atgcttgtaa gagtaccaac agtagagcga ggattttggc
     7681 ttcctttttt ctgttcgata gctatagtag gggttaatcc ctgaagtaaa tcaaattccg
     7741 gtttttgaag ctgttctgaa agttttgctc cattacttag attttcaaaa taacgtcttt
     7801 ttccttcttc ataaatcgta tcgaaagcta aagaagattt tccactacca cttacacctg
     7861 taataacagt tatttgattt tttggaattg taacctcaac atcctttaaa ttgttttgcc
     7921 ttcctccttt taaatagata gagcttttat tatgacaaag tgaattttta attgatttat
     7981 ttattgattt tgtaaatttg aatttctttt cgagttcaag aggattgcct aattctttta
     8041 tttccgaaat gcctaatatt tttaactgag aaaagattga tttcaaataa tcttcaggca
     8101 atttttctat taacaaatta aggtcttcta tttttatatt tccgcttgat aatatttctg
     8161 ccatttcatc taataaattt ttttctgctc ttgttaattg attgtatttt ttggaactta
     8221 attctgtcca taaatcccat ggaagcaatt ctaaaccaat catattatta aagtcatgta
     8281 gaagattacc ctttacagaa tgccaacctt tccaattttt attatatcca aagtcatctt
     8341 gatgagcatg tccttgccga catcttatcc aggcttctga accagttaaa aaaccatctg
     8401 tttttccgac atcatgagtg tcaaaattaa tttgaaaata atttttttgt atgtcatcta
     8461 tttgtgcatc tattcttatc catctctttg ttagagtatc atagtattct acaagccagt
     8521 ggtcttcata agtaagctca ctttcaaaat aattagcaaa acctgctctc attctagcct
     8581 caaaacctcg atatcttagg agcgatacaa gaagtaaaga ataatctctg cacattccaa
     8641 caagcctttg ttttgggttt cttggaatag ttagtgggtt agaatttatt tcaaatattc
     8701 tttttaacat ttgaggaatt gtacgaagca attcctcatc agattgttgt ttattaaagg
     8761 atataccata taattttcct tggtctccat gaattactag gctttgaacc acttttaata
     8821 actcaattgg agtctttggt aaatcttgat atttattttc aaatttttta ggatttgtga
     8881 tgatgctttg atttttatag ttatttattt tcatttatat ttcacctctc taaatggatg
     8941 atataccctg acatggtgtc atatgcaagg gcaatttaaa ttttttggcc aaaatttaaa
     9001 aataaataaa aaatgataca gtgataaatt gtaatacaaa tatagtttca aattgcatat
     9061 aatataatta tttaataaaa ccattatcta tggaatgtta atgaaatgtt aagatggcat
     9121 tcttgaatat taagaaatag aatactataa tatagtgtga aggattttta gtaaaaaaat
     9181 ttgtaagtgt taatatacat taaaaaatag atttataatt tggaggaaaa aaatgaaatt
     9241 atcaaggtat aagaaaaata gtttagttct gtctataatt ttatgttcac ttattatttc
     9301 aatccttgta attactgttt ttaataccgt aaaactacac aatatatcta ataaacaatc
     9361 taaaaactat ttaaatgatg tatctacaca gattgtaatg aatgttgatt ctaaaatcaa
     9421 atttatttta tccgacttaa gaataatggc agattttatt aagcaatatg aaggtgatag
     9481 tcgatatgag tatttatcta aaagaaagac tagttataag tattatgatg ttggaataat
     9541 agatttagaa ggtaaagctg agtttttaag tggaaaaaag tttgacttaa agaatactgt
     9601 ttcctataaa aaaggaattg aaggtaaaga atactcagaa gtaatagagt caatgggttt
     9661 tgttctttat agtgtaccta tatttaatga taatcatgaa gtatctagta ttttaatagg
     9721 tgtatccaat aaagaaagta tgaatgagat tcttaatata gataacttta atggaaaagg
     9781 aactattgaa attattaata gtaagggaaa acctcttttc ataggaaaaa atactgaatt
     9841 aataaaggac cttagccata aatataagag ttctggagat gaaccttggg cacaaaagat
     9901 gcttgaggat tttaaaaatg ataaatcagg gaatattaca ataacctcat caaaaggtgt
     9961 acaatgctta ttaacttatc atccaattac aagaggttta aatgattggc attttcttct
    10021 catagtacct gaagatgctg tacttggtga acttaataaa ttaaactctt ttacaataac
    10081 aatgacattt ataattacat gtattattgg aattataaca ttaattttat atattataag
    10141 aagaaaatat gtaaatcaaa tagaaaatat tgcatattta gatagtataa caaatggtat
    10201 aaattcaact aagtttagta tgctagtcaa acctttaatt tcagaatcac ctgatagtac
    10261 ttatatgatg atagcaatga atataaaaga ttttaagcta ataaatgatt gtttcggtag
    10321 tgaaaaggga aatatgacac taaaacatct ttacaatatt ttgtataaaa acattgatga
    10381 aaatgaagag tttgtgtgcc gtcatgatgc agaccttttc tatttgctta ttaagaatag
    10441 accagtgtta gaagccctgg agttattata taaaatagaa gctgacacaa attattttaa
    10501 taaaaatagg gaaaatccat actttttaag actatcagta ggtatttaca caattgaaaa
    10561 tcataatgaa gatttaatta caatacaaga ccatgcaaat acagcaagaa aaagctttaa
    10621 taaaactcat caaagtgatt tttcttttta tagtgatata gaaagaaaac gattaatatc
    10681 tgaaaaggaa atttcaaatt taatggaaaa agcccttgaa aataaagaat tttttatgtg
    10741 cttacaacct aaaattgata taaaaagtgg aaaaattagt ggagcagagt ctcttgtgcg
    10801 ttggaaaaat tcagaaaagg gcataattta tccaagtgat tttattcctc ttttcgaaaa
    10861 aagtggattt atatgtaagt tagatttata tatactagaa gaaacatgca aattgatttc
    10921 taagtggata aaagaaggta aagaagtgtt gagaatttct gttaatgtat cacgtcaaca
    10981 tctgaatgat aaattgtttt tagaaaaata taagcatata tgcaataaat acaatgtacc
    11041 aacatgtctg atagatttag aacttactga atctattttc ttagaaaatc cagaagctat
    11101 agatataata gaggatattc attctaatgg atttaaatgt tctatagacg attttggatt
    11161 tgggtactca tcattaggga tattaaagga ttttaaagtt gatataatta agttagaccg
    11221 ttcattcttt gttagcaaaa ataatataga ccgtggtaag gtagttatta agtctattat
    11281 tgaacttagc aaaaggctag gtatgaaagt aactgctgaa ggaattgaag aattagaaca
    11341 agttgagttc ctaaaaaatg tgggatgtga ttatattcaa ggttatgttt tttcaaaacc
    11401 attattaatt tctgactttg aaaattttgc atatgaaaat aataaaatta aaaatatcca
    11461 ttattaaatg tacaagataa aaaatttagg caagttatat aattaaaaat ataacttgcc
    11521 ttttttattt atatatttaa taaaaatata tttgcagatt ttgtaaaaat aattttcaaa
    11581 tgttgtaagt tgtggctaaa gctttattgt attattctga atatagaaag atagttactg
    11641 tcttagataa aaactgaaac atacataata atataaaatt tgctatatta ataactctaa
    11701 ttattttgga aggagggaaa atatgataag tgggagaatg ttactcatca tgaattttct
    11761 aaaaggaaaa aataagacaa gttacaagga tattagcaaa caacttagta tagaagaaag
    11821 aaaagttaga tatgatataa ataatttaaa tattgttttg cgttcattaa acaagtcagt
    11881 aatacaaaag atgaacaaag gtgtgttaat tattcctgat gacttccaag tagtatgtat
    11941 aaataataac gaatatgtat tttccttaac tgagcgagta tctattatga aaattatatc
    12001 aatgtttaaa atagataatc taaacttaga aaaattaagt aaagaatttc aggtttctag
    12061 aagttcaatt aaaaatgacc ttaatgtttt gtcaagtgaa ttagaaagaa accaaataac
    12121 tattacttat gataaatctt ttaaaataag tggagaagaa gacattatat ataagcaaag
    12181 attgaacata ttaaaatcgt attcttatct ttttggtaag gaaaaatctg atttaagtgt
    12241 atttgagaag tatttagtga atgtaattga aaaaatattc aaagggattt atttgattga
    12301 tatatataat tggtctatga atttattatt gcaaatggga tggactttaa atgatgaatc
    12361 ttttacatgg tatgtgtcaa acattttcct ttttacatgg tatatacatt cagatgaaaa
    12421 acatccttta gaatctgctt cattaagtga gccgtgtttt gaaaataagt ttgaccaaga
    12481 atttgaaaca attattcaaa aaaagttgac taaggaacaa atttttctgt taaagagttt
    12541 tgtatttttt acaaataaat atgcaagttt aaacgaggat atggatttga ttagtacaga
    12601 aattatagtt caagacctaa tttacaatat gtcagaaata ttagagatta gctttaaaga
    12661 agatatgatt ctttataaag gattactcaa tcatattgcc ccattgatag agagaatcaa
    12721 gcagaatata cagatttatg aggaattact tcatgtaata ccagataaat atcaatatgt
    12781 acttgaagct actaaggttt ctattcaaaa aaacagagtt ttaagtcaaa taaagaatga
    12841 aaatgaaaca atacttttgg caatacattt cttaggaagt atacatagaa atgagaggaa
    12901 taactacaaa aatatattgt tagtttgtgg atttggatat ggtgtaattg ctatgttaaa
    12961 ggatagttta gtaaatgatt accagataaa tatagtagag agtattccat catataagtt
    13021 acaagattat aaagactggg aaagtattga cttcattata acaacatcaa agatagtaat
    13081 agacttgcct aaaacaatta ttgagataaa tccattttta caagaagagg attatttgaa
    13141 gtttgaagaa aaagggataa aaagaaagaa tgtcttaact aattatatgt caataaaaaa
    13201 aaggttggac tttttagaag aagatgccca aaaaagagta ttaagtatta tatgtcaaga
    13261 acttggatac agtgatgaga gaatattatt tagacaactt aatttaagtg atttaatagg
    13321 gattgatacc ataaggataa ttgatggaga tatgaaatgg gaagatgctg taaaatttag
    13381 ttcaaacata cttgctaatt gtgattttgt atcagcacaa tatgctgaga atattataga
    13441 tattttaaaa aatgtgggtt tttatgctgt aaaagataac gaatttgctt tactacatgg
    13501 caatgatagt tcattagtca aagtaagttc gataagttta ttaatatgta aagaagcagt
    13561 tagttttgga gataaaaagg ttaaaatcat attttgccta gcaagcagag ataaaaaaga
    13621 gcaaattcct gcaataatta atctaactaa aatggtttat aagactaatt ttattgctat
    13681 gttagaaagt gcaaagacaa aagaagaagc agaagtttta attcataaat atgagaagga
    13741 ggtcactgta tgaataaatt agttaatgta aaatgttcat ttaaaataca tgttaattca
    13801 aaagaagaag ccattatatc gttagttgat gtagtgtcaa aagaaggata tttaatagat
    13861 aaaaatcaat ttttaaaaga tgttttaaag agagaagaaa cattgtcgac ttatattgga
    13921 catggtatag gtcttcctca tagtcaaagt gtaggtgtta aaaactcttg tattacaata
    13981 ggaaagcttg atactcctat tgaatggaca gaagaaggag aaaaagtgga tttaatattt
    14041 ttgatatcag taacaaaaga caatgaaaat aatcttcatt taaagatttt atcaaaatta
    14101 gcacgtttat tgatgcatga aagttttaga aatcaaatta gatgttcaga tgaacaaact
    14161 gtttataatc ttataaaaga aaaaatagag gaggaagatt aaaatgagaa ttttaaagtt
    14221 tttgcaaaaa catcttatga cagcaacatc ttatatgatt ccatttgtgg ttgcaggagg
    14281 aatattattt gctttatcag taactttaag tggacaagct gctgttcctg aaacaggctg
    14341 gttggctaaa ctgaatcaaa ttggagctgc tggcttggct ttattcattc ctatattagg
    14401 tggatatata gcatttagta tggcagataa accaggatta gctccaggta tgataggtgc
    14461 atatttagca aaagaagtaa atgcaggttt tataggtggt atcatagcag gatttatagc
    14521 aggatttgtt gttttacaat taaagaaaat taaattagct cctacaatga gaactttagg
    14581 ttctatattt atttatcctt tagttggtac attgattaca ggtggtataa ttgtattttt
    14641 aattggagaa cctatagctt cttttatgac atggatgaca aactggttaa atggaatgtc
    14701 tggtgtttct aaaattcctt taggtggaat cttaggagga atgatagcct cagatatggg
    14761 tggccctata aataaggttg ctgctacttt tgcccaaact caagttgaca cacttccata
    14821 tttaatgggt ggagttggcg ttgcaatttg tatacctcca attggtttag gattagctac
    14881 attattgttt ccaaaaaaat ttagcaagga agaaagagat tcaggtaaag cttcactatt
    14941 aatgggatgt gttggaatta cagaaggagc tattccattt gcaacatcag atccagttag
    15001 agtaatacct tgtattatgg ctgggtcgat tgtaggaaat attatggctt ttttattagg
    15061 atgtttaaat catgcaccct ggggaggtct gatagtgtta cctgtagttg acaataggtt
    15121 ggggtatatt gcatcagtat taactggagc agtagttgtt gcggtattaa tgaaattagt
    15181 aaaaaaagac gtgaaagaag atgaagaaat tgaagaagat ttagatgaat caattgaatt
    15241 aatatttgaa gaattataaa tataaatgga aggaatttgt ataaaaaagt aaatataaaa
    15301 ctataaattt aaaggagata agaatatgaa aattgtagca gtaacatctt gtcctagtgg
    15361 agtagcacat acttacatgt cggctgaatc acttgaatta tcagccaaaa aatttggtat
    15421 agaaataaag gttgagactc aaggaagctc tggaatagat aatgaattgt cacttaaaga
    15481 tattgatgaa gcaacttgtg taatattgac taatgatgtt gagattagaa atatggaacg
    15541 ttttaaagga aagaaggtag taagaatgag tgtatctgag ataattaaga aatctgatgc
    15601 attaattaag aaaataaaag atacttttca ataaaaattg atttatttta catataactc
    15661 aaagatgttt ttaaagaaat ttttattaaa agcatctttg aaaatatctt aaaaatagaa
    15721 taaaaggaga tatagtcatg aaaaagttac caataaagac tgtcgtgcaa agattactaa
    15781 acttacaaga agaggggaaa agtgcaactc ttttaggaat tggtcctatg tcaccaaact
    15841 tgttacaagc aagttttgaa ctagcaaaag atgatgattt tcctttaatg tttatcgcaa
    15901 gtagaaatca agtagatgca gatgaactag gtggcggtta tgtaaatggt tggaatcagg
    15961 aaacttttac aaaagatatt aagaaggttg ccgacaaagt tggatttgat ggtttatatt
    16021 atttatgtag agaccatggt gggccatggc aaagagataa agagagaaat gaccatttgc
    16081 ctgttgatga agcaatggag ttaggaaaaa aatcttattt agcggacatt gaagcaggtt
    16141 ttgacctatt gatgattgac cctacaaaag acccatttga aattggaaaa gtaattccac
    16201 tagatgtagt actagaaaga actgtagaat taattgagta ttgtgaaaac gagagaaaaa
    16261 gacttaatct accagacata ggttatgagg ttggtacaga ggaaaccaat ggaggtctga
    16321 cttcaactga gacgtatgaa acatttataa ctagactaaa ggttgaactc gataatagag
    16381 gtttgccaat gccaacattt atagttggtc aaactggtac tttaactaga aagacagaac
    16441 aagtaggaac ttttaatttt agaaatgctt atgatttagc tcaaatggct aagaaatatg
    16501 gagttggttt aaaagagcat aatggcgatt acttagatga tgtaacttta ttagaacata
    16561 taccatctca aattattgct acaaatgtag ctcctcaata tggtactgaa gaaactaggg
    16621 catatttaaa attagcagaa gttgagtgta aattagaaaa agagggctta gttgagaaaa
    16681 cttctaatat cagacatgtt cttcttgtta atgcaattga atgtggacgt tggagaaaat
    16741 gggtagtagg agaacaaaaa aatctaacta ctgaagagat atttaaggat gaagttttat
    16801 ctaatgaaat actagatatt gcaggacatt atacatttaa taatgatgat gttaaaaaag
    16861 aaatagaagt gctttatgat aatttaagta agaataatat tgatggtcaa agatttgttg
    16921 tagaccatat taaacgtcct ttgcgtgatt atgcagaatg ctataactta aaaggtgtta
    16981 ctacgaggat tttaaataaa taataggact tatagtggtg agtattgtta tactaggtat
    17041 ttatagttat aattatactt accactatta aatttaggag gactaatatt ttgattaatt
    17101 taatttttga tgtagatgat actttatata atcaattaac gcctttttac actgcatata
    17161 ataaagtgtt ttcttcaata aaggatattt ctattgaaga tttatatatg agtagtagaa
    17221 agtatagtga tgaggtattt catatgactg aaaatggaga aatgccaatc aaagaaatgc
    17281 atatttatag gataatgaaa gcttttgagg agttgggaaa ttctattaca gaaaaagatg
    17341 cacaaagttt tcaagatgag tatatatatc aacaatcaca aattacacta ataccagaag
    17401 ttgagaggat tttaaatttt tctaaagaaa gaaatattaa tctaggaatt ataactaatg
    17461 gcccatcaaa ccatcaaaga atgaagttaa agcagttaaa tattgaaaac tgggttgata
    17521 aaagtaatat ttttatttct agtgaggttg gattttctaa gccagatact aatattttta
    17581 gagtagctga gaatgttatg aatttagata gagaaaatac atattatgtg ggtgattctt
    17641 atagaaatga tgtattaggt gctaaaaaag ctggttggaa gagtatatgg ttgaaccata
    17701 gaggacatga ggtagaagag ttgttttaca aacctgattt tgtaattttg gaacataagg
    17761 atttgatatc tttatttata aaaatatgta gttataaaaa tatgtagtaa aaaaatgtta
    17821 ataatagaat tgtataaaat aattaataat aactatataa aattatatgt ttagattgtg
    17881 agcaatctat tttatgttaa aaatagatta tttagaagaa ggggaaatct taaaaaaatt
    17941 tgaatatccc ctttttatta tttaattaaa atgaaatagt tagaaatata ttttatttag
    18001 tttagtcaat aacattttgg gattctttat aactacatgt taactgttga ataggtttat
    18061 catatggttt aacccatcct ttattaatac ttaattctgt aagtgctgtg tgaccttcta
    18121 ccatttgagt taatgaagct gaatatatag ctcttaattc aggtgtacta cttgtcaatg
    18181 cagaattaag atacatattg gcagatgctt tagcagatga aagcatactt aacattatta
    18241 ctttatcgtc tatataagta ttgttcttaa ttagattttc tatcatattt ctcatgttat
    18301 ctatacctcc tcagaaatta gtatttcatt ttcaactata aattgttgca tagctttaat
    18361 tcttccttct gttgctaata tactagattc tgattgtctt tttaggtctt catctgatat
    18421 taacatattt attgctcttt gcataagtaa tccgtcagac tccattttta taactgctgc
    18481 taaagatatt ttttcagttt ctgaaagtat tctcatttaa tcatctccta tattgtaatt
    18541 tatgctttaa tagtatgtct ttcttttcaa ggaaataacc attacacttt tttacagtat
    18601 tttatattct ctttatatca aaatttagtc gacctaatat attaataata attttgtagg
    18661 atatttatca ttaaatatct ataaaataaa ttatttgaat attcataaag caaaatttat
    18721 ttgtatgtac ttcttcaaga gtcaatatga gttttttcaa aaaattaatt tttatgataa
    18781 tcattaaggg ctatattttt cttaaagttg tgattatcat tattaattta caataaaatt
    18841 gccgaaaagc cctctccttt aggtgtaggg atgaaaggta aggtcttaat ataaaaaaag
    18901 ttagaataat gcttgtaatc taataccgct actgataccc tatatttagg aggtgttagt
    18961 ttttatgtat aaatctaaca aaaatatagt ttatgattgc aaatatcata ttgtattttg
    19021 ccctaagtat cgtaaacctg ttttagttgg cgaagttgaa aagatgttaa aagaaatact
    19081 accatataaa gcagatgaac ttggagcaga aataatagaa atggaaactg ataaagaaca
    19141 tgtgcactta ttaatatctt gtgaccctca atatggaatt cataaagtag taaaaggttt
    19201 aaaaggcttt agttcgagag tattaagaga gaactttcca catttaaagt ctagtatgcc
    19261 tagcatgtgg actaatagct attttgtagg tactgttggt tctgtgagtt tagaagtagt
    19321 taaacaatat atagaaaatc aacaagttag gacaaagaaa tgataaaaac tcatagagtg
    19381 aaattaaacc taactagaac acaatttgaa ttagtaaggg aaaagcaaat ggaaagtgct
    19441 aactgttgga actacatagt taacttatct aaagaatact attttgaaca taaacaatgg
    19501 attaaaaaga atgatataca aaagttaatc aaaggtaagt acaatcttca tagtcaaact
    19561 attcaagcta tatcagataa atttgatgca aatagaaaga ctatatcaga attaagaaaa
    19621 aaaggtaata ctaaagcaaa atatccatac aagactaaga aattttatat aatacctttt
    19681 aaagctagtg ctattaatag aaatgctaaa ggcaacttaa aattatcaat gtcaaaaggc
    19741 aggtatttag aattagattt taatgttgag aatataaaaa ctgctgaaat agtatggcga
    19801 aatggatatt atttatacta cacttttgac aatgaattaa atggagttgt agccaaaggc
    19861 actaataccg taggtgtaga cttaggggaa atacattcga tagctagtgt aacaaatgaa
    19921 ggtgtagggc ttattctatc taatagagaa ggcagaagta ttaaacaatt tagaaataag
    19981 atgtatgctt atatatctaa aagattaagt aaatgtaaaa aaggtagtag acaatcaaag
    20041 aagttatgga gattaaaaaa taagataaga agtaaaacag ataatcagtt aatgaattta
    20101 tatcatcaaa caactagaaa gtttatagac ttttgtgtag agcagaaggt atgtgaaata
    20161 gttcttgggg atataaaagg ggttgaaaaa gatactaaga aaaagaaaag attaaacaga
    20221 gttaatagac agaaaatatc tcaaatggag tatggtcgaa taaaggacta tataaaatat
    20281 aaagcaaaag agcaaggtat tgaagttaag ttagttaaag aaaactacac ttctcaaact
    20341 tgcccaaagt gttctaaaaa acacaaacca actggtagaa cttatagttg tacttgtggt
    20401 tatgaaactc atagagatat agttggtgct tggaatattt taaataaaaa gcataaatat
    20461 ggtttagtag attttagaat aaatcataag caaccaataa atttaaaagt atctaccgtt
    20521 tgagccatgt gtgggcgaat aactttaagg tagtagtggc gaagacaccg tccgaagtag
    20581 cttggctttg taaccacctc tgttgtactg tttaagtata aataagtgtg ttagccacca
    20641 agaatcccct tgctttagct atggggagtg tgtcaataaa atactttaat gaaaaatagc
    20701 taactttaat tgtaggatat tcttaatata tttgaaataa ataaaaatat tgaaggatat
    20761 aatattcaat tggtatataa ataataaatg atagttaaat tttaccttag aggctaatta
    20821 ggtgtttagt caaagctaat gcctttagaa tacaaatgta ttctaaaggc atttttacta
    20881 tgtattttca tttatattac taaacattat ctatttttat atacatttct atgtatttta
    20941 ttttcatcaa atcggtctat aaactcattt ttttcttcac tatatccaat agatataagt
    21001 gcataaggaa ctaattttaa tggtaagtct aaagtttgtc taactttatt taccacttta
    21061 tttttaggaa atactcctgc ccaacaagca gctagacctt gattagtaac ttctaataat
    21121 aaattttgtg tgcaagcacc taaatcttgt atccatttac caggttttaa gaatttatct
    21181 ctatttccaa gcactactat acataatggt gcaaattcta tatgctttgc tctatgctgt
    21241 gactttgaga gctgtttcag cagttcctta tcatctacta taataaattc ccaaggttga
    21301 gcattttttg atgaaggtgc ttgcatagct gattttagta tttcagttat tttatcttct
    21361 tctatatttt tatctatata tgttcttatt gaacgtctat tttttataac ttctaacata
    21421 cagattctcc tttaaataaa attatttcta tttatataat atcattactt taaattttag
    21481 ctattactta tataaagaat aaactttgtg ttggttttat ctaagtaaaa gtaacattca
    21541 gaactaaaaa acaagactat aaatccaaag tattaattaa aaaagaagta ataaaaaata
    21601 atatacaaga tttaaaaaag agtattgggc ttaaaattta attagaaaac caattttgag
    21661 ataatctgtt ttttatttta acaaaaaaac acaaataaag ttcgatacgt agcgaatgaa
    21721 aaaattacaa gaaatatttt ttttaaaaaa tttcaaaaaa gagttgataa tatatgtata
    21781 ttaatataaa atttaaggtg tataattcgt ataataatac gaatgatttg tagtgtaaaa
    21841 taacttataa acggagaggg gaagtctaat gaaagtagca gtagttatgg gttcaaaatc
    21901 agattatcca aaattagaag aagggataaa actgcttgaa aaatatggaa tagaagttgt
    21961 agcaagagca ttatcagctc atagaacacc agaacaactt tcaatattcc taaaagaaat
    22021 agaagatgat actgatgtaa taatagcagc agcaggaaaa gcagcacact taccaggtgt
    22081 aatagcttca caaacattaa ttcctgtaat aggattacct attaaatcat caactatgga
    22141 tggactagac tcacttttat caatagttca aatgccaaaa ggaatcccag ttgcaacagt
    22201 tacaatagac ttaggtttaa acgcagcttt attggcatta caaataatga ctttaaaata
    22261 cccaaaatta aaagaggatt tgaaatcata tagagaagag atggcacaaa aggtgttaga
    22321 agacgacaag aatttaaggg ggtagcagaa atgttattat atgaaggaaa agcaaaacaa
    22381 gtatattcta cagataatga aaatgaatat gttgtttatt ataaagatga tgcaacagca
    22441 tttaatggag aaaaaaaagc agaaatatct tcaaaaggta tactaaataa taagatatca
    22501 acaataatct ttgaaatgtt aaaagaaaat aatataaata ctcactttat aaaaagttta
    22561 tcagatagag aaatgttagt taaaaaagta gagattttac cattagaagt aatagtaaga
    22621 aatattgctg ctggttctat ctgtaaaaga gttggtcttg aagaaggtgt agttttcgat
    22681 gagccaattt ttgaaataag ctacaaaaat gatgcttatg gtgacccaat gctaaatgat
    22741 gattatgcag tggcaatgaa gttagcaact agagaagagt taaaattctt aagagaagaa
    22801 actttaaaaa taaatgagtt actaaaagca tttttcttaa aattaaacct aaagttagtt
    22861 gactttaaaa tagaatttgg taaggattct gaaggaaata taatattggc agatgaagta
    22921 tcaccagaca cttgtagact ttgggatgta aatacaaatg aaaagttaga taaggacaga
    22981 tttagaaaag accttggaga ccttgtagaa ggttatacgg aagtactttc aagaatgaat
    23041 aataaatagg aggcaaatgt atgtgtggag ttttagggat ttactctaat aaagatgtaa
    23101 ctaaagaact atattattct ttatattcta tgcaacacag ggggcaagaa agctgtggtt
    23161 tagctctttt agatgatgga gaaattaagt acaaaaaaga tatgggatta gttggagatg
    23221 tttttaagga aaatgaatta tctaagttaa aaggaaatat agggattgga catgttagat
    23281 attcaacagc tggaggaagt catgtttcta actgtcaacc tttagttgga agttgtagaa
    23341 aaagacaatt agccatagct cataatggaa atctagttaa tgcaaattat ttaaaagata
    23401 tgctagaaga agatggatat atgttccaaa ctaattcaga tacagaagtg attttatata
    23461 tacttgctag atattacaaa ggggatatag tagaaagttt aaaagtgaca atggattata
    23521 taaaaggtgc atatgctctt gtaataatga gtcaagaaga attggttgca gttagagacc
    23581 cacatggatt cagacctctt gtacttggta aaaagggtga tgaatatata tttgcatcag
    23641 aaaattgtgc aatagatata cttggcggag aagttataag agatgtagag ccaggagaga
    23701 taatagtagt caaagatgga gaactaaaat catattttta ttcagaaaat tataaaccag
    23761 ttaagaaaag ttgtatattt gaacacatat actttgcaag aaatgatgca actatagaca
    23821 atgtaaatgc atatgaattt agaattaaat gtggtgagag attagctcag aatgaaactg
    23881 taaaggcaga tatggtagtt ccagttccag attcaggttg gccaggagct attggttatg
    23941 caaatgctag tggattaaaa ataagtgaag gcttagttaa aaatagatat gtaggaagaa
    24001 catttataaa acctacacaa gaagaaagag aaatagcagt aaaaataaaa ttaaatcctc
    24061 tttcaacaat aataaaagga aaatctataa ttttagtaga cgactcaatt gtaagaggaa
    24121 ctacatctaa acaattagta aaatctttaa gagaagcagg agcaaaagaa attcatctta
    24181 gaataacttc tcctccagta gcttactctt gttactatgg aatagatact ccaaatcgct
    24241 ctaaattaat tgcatcaagt aacaacgtag aagaaatgag agaatatatt ggatgtgata
    24301 gtttaaaatt cctagatata gaaggaatgt tagatgcaac agaacataag tctactttct
    24361 gtaaagcttg ttttgatgga gaatacccag ttaagaaaat tgataaggag gaactacttt
    24421 catgttaaca tataaagaat caggtgtaga tatagatgaa ggaaatagag cagtagacct
    24481 tataaaaggt aaaataaaag gaacttatga tggaaatgta gttggagatt taggtaactt
    24541 cagtggatta tacagtttaa aagattttgt aggtatgaaa gaaccagttt tgcttgcatc
    24601 tacagatggt gttgggacta agttaaaaat agctcagatg atggacaagc atgatactgt
    24661 tggaatagat ttagttgcaa tgtgcgtgaa tgatttaatt tgtcaaggag caaaaccatt
    24721 attcttcctt gactatatag ctcttgggaa attagtgcct gagcatatcg aaaaaatagt
    24781 tggtggaatt gcagatggtt gtaaaatgtc tggttgtgca ctaattggtg gagaaactgc
    24841 tgaaatgcct ggaatgtatg gagaagatga ctatgattta gcaggatttt ctgtaggaat
    24901 agcagacaaa gaaaagattg tttctggaaa caatgttaaa tcaggagatg tattggttgg
    24961 tatatcttca agtggagtac acagcaatgg attttctttt ataagaaaga tatttttaga
    25021 aacttacaat tataaaatgg aacaatatgt tgaagaacta ggaatgacag ttggagaagc
    25081 acttttaaca ccaactaaaa tatatgttaa attagcttta gatgtattag ctaaacatga
    25141 cataaaagct atagctcaca taactggtgg agggcttata gaaaatataa caagagttat
    25201 acctaaaggg ttaggactag atatcaataa aaaatcttgg gagaagcctc ctatatttaa
    25261 aatgatagaa ggttttaatg ctgttgatga gagagaactt cataagagtt ttaacatggg
    25321 tattgggcta gttttaatag tagataaaga aaatgctgat gatgttgtaa actttataaa
    25381 taatagagaa aatgacaatg ctgcttatgt agataaaaaa tatagtgaat tattagaaga
    25441 taaagcttat ataattggtg aagtagtaga tagtcatgaa ggtgtagaat tatgctaaat
    25501 ataggagttt taatatctgg tggaggaaca aatttacaag cagttataga tggtactgag
    25561 tctggagaaa taaaaggtca ggttaaagta gttatctcaa gtaagcaagg tgcttatggg
    25621 ctagagagag ctaaaaatca taacataaaa gcaatttgtg aaactgatga agacaagata
    25681 atagaaattc ttaaggaaaa taaaatagat ttagtagttt tggctggtta tttaaaaata
    25741 atcagcccaa agctggttaa cgagtttaga aacaaaatga taaacataca tccatcatta
    25801 attccttcat tttgtggagc tggattctat ggagaaaaag ttcatcaagg tgttatagat
    25861 tatggagcta aagtaactgg tgctacagtt cattttgtag atgaaggtgc tgatactgga
    25921 ccaatcataa tgcaagatgt agttaaggta aatcaagatg atgatgcaaa gacacttgca
    25981 aaaagagtgt tagaagttga acataggatt ttaaaagaaa gtataagtct tttttgtgaa
    26041 aataaattaa aacttcaagg taggagagtg tttataaatg agtaagagag ctttaataag
    26101 tgtaactgac aagactggtg tagtggaatt tgctaaagag ttaaataaat tagattatga
    26161 aattatatct acaggaaata catttaaaac attaaaagaa aatggtgtta atgtaatgca
    26221 agttgaagat gttacaaatt ttccagaaat attagatgga agagtaaaaa cattaaatcc
    26281 atatatacat ggtggtatac tttataaaag agataaagaa tctcatgtag aaactgtaaa
    26341 tgaacacaaa atacattcaa tagatttagt agctgtaaat ctatatgatt ttgaaggaac
    26401 tttaaaagct ggaaaaagtc atgatgaaat aattgaaaat atagatattg gtggaccatc
    26461 tatgatacgt tctgctgcca aaaactataa ggatgtaata gtagtagtag atataaaaga
    26521 ttatgattct ataatagaaa aattaaaaac agatacaatg acattagaag acagaaaaaa
    26581 attatcatat aaagctttct caactacagg aagatatgat gcgttaatat ctagttattt
    26641 tgctggagaa gttggagata cgtatcctga catattaaat ttaactttcc aaaaagaaca
    26701 aactttaaga tatggagaaa atccacatca aaatggtttc ttatattcac agtcaaatgc
    26761 aaaaaatcca atcttaaatt atgagcaatt aggcggtaaa gaactttctt ttaacaactt
    26821 aaatgattta catggatgtt tagaagtaat gagagaattt aaagatagtg aagaagtagt
    26881 ttcagtagct ataaaacatg ctaattcatg tggggtaggt ttaggaaaag atgcttttga
    26941 agcatataca aaatgttatg aagcggataa ggtttcaata tttggaggaa tagtaggaat
    27001 aacttctact atagataaag ctacagcaga gaaattaaat gaaatattct tagaaatagt
    27061 agtagcatat gattttgaac cagaagcttt agagatactt aaacaaaaga aaaatctaag
    27121 aatattaaaa ttagctaaaa tagaaaatag tttacaacca tatgaaatga aatatttaga
    27181 tggaaaatta ttgatacaag atagaaacaa tatattagca gaaaaaagtg aaaatgtaac
    27241 aaaggaaaaa ccaacagatg cacaacttaa ggacatggaa tttggtatga gagtagtaaa
    27301 aaatatgaaa tcaaatgcaa ttgcagtagt aaaaaatgga caaactctag ctttaggttg
    27361 tggacaaaca tcaagaattt gggcattaaa aaatgctctt gaaaataaca aagataaaga
    27421 ttttacagga gctgttttgg cgtcagacgc atttttccca tttgatgatt gtgtaacatt
    27481 agctcatgaa tatggaatat cagcagtagt tcaaccaggt ggctctataa aagataaaga
    27541 ttcaatagaa gcttgtgaca agtatgatat ggttatggta tttacaggaa taagacactt
    27601 caaacactaa ggaggatatt atgaagatat tggttgtagg cggtggcgga agagagcatg
    27661 ctatatgctg gaaactaagc aaagaaaaaa atgtagaaaa aatttattgt gcaccaggta
    27721 atgcaggaat tgcaaacgta gcagaatgtg taaatatagg tgatactaat atagaagaac
    27781 ttttaaaatt tgccaaagaa aatgaaatag gtctgacaat agtaggacca gaagtaccac
    27841 tagttatggg aatagtagat gagtttgaaa aagaaggatt aagagtattt ggacctaata
    27901 aaaaatgtgc tcaacttgaa ggaagtaaag ctttttcaaa agaatttatg ataaaacata
    27961 acatacctac tgcaaaatat aaagaatata caaacttaga agaagctata tcagaaatag
    28021 atagttttgg atatccagta gtaataaaag cggacggatt agctgctgga aaaggtgttg
    28081 taatacctga aaatagagaa gatgctatag caactttaaa agaaatgatg tcagataaga
    28141 agtttggagc tgctggagat aaaatagtaa ttgaagagtt tttaaaagga atagaaactt
    28201 caattctagc atttgtagac aatgatacaa tagttcctat ggcaagtgca aaagaccata
    28261 aaaaagtaaa caactatgag caaggtccaa acactggagg aatgggaact ttttcaccta
    28321 gtgaaatata tacagaagaa ttagcaaata aagtaaaaga gacagtatta gaaaaaactt
    28381 tagaaggttt caaaaaagat ggacttaatt ttaaaggtat tttatttgtt ggtcttatga
    28441 taactgaaga tggagaaaaa gtacttgaat ataatgtaag atttggagac cctgagacac
    28501 aatctgtttt atttagatta gaaacagatt tacatgagat aatggaagct atactagata
    28561 ataagttaaa agatatagaa ataaactata gtgatgatga agctgtatgt gttatgctta
    28621 catcaggagg gtatccagac agttatgaaa aagggaaaat tataactggt cttgaaaact
    28681 tggatgatga tatagtagta ttccatagtg gcactaagat gtttgatggt aatttggtta
    28741 ccaatggagg aagagtaatt ggtataactg caaagtcaac tagagttaag gatgcagcag
    28801 aaaaagttta tgaaaacata aagaagataa actttgaagg aatgcactat agaacagaca
    28861 taggaagata atttttctaa aatggaggat aaggtatgtt gaatactgaa aataaagact
    28921 caatggttag aagagtattg gttgaaaaaa gagaaggttt tgaccttgag gctaaagctt
    28981 taaaaaaaga cttagtagaa agtttacaca tagataatat agaaaattta agaatattaa
    29041 atagatatga tgtagaagga atatcagaag aagtttatga aaatgcagca aagacaatat
    29101 tctctgaacc aaatttagat gtagtatatt atgaagaaat accaaaatta aatgatgaaa
    29161 gagtatttgc aatagaattt cttcctggtc aatacgacca aagaggtgat tgggcagctc
    29221 aatgtgtaca aattgtaaat caagggataa gacctgctat taatacagca aaagtttata
    29281 tattatcagg caaaataact gatgaagagt tttctaaaat aaaagattac tgtataaatc
    29341 cagtagatag tagagaagca tctttagaga aaccagaaac gttaaaaatg gaaactgaaa
    29401 taccaacaac agttgaagta ttagatggat ttatagattt agatgaaaat ggattaagaa
    29461 catttgtaag tgaaaaagga ttagcaatga ctttaggaga cttacaacac gttcaaaaat
    29521 actttaaaga tacagaaaag agaaatccaa ctataacaga aataaaagta ttagatactt
    29581 attggtctga tcattgtaga catactactt ttatgactga aatagaaaat gtaaagatag
    29641 aagatggtaa gtttaatgat atagtaaaag aagcgtatca aatgtatctt aactcaagag
    29701 acaatgtata tgtgaacaga cataaagata tatgtttaat ggatatagcc actgttgcag
    29761 taaaagaatt aaagaaaaat gggaaattaa atgacctaga tgaaagtgaa gaaataaatg
    29821 catgtagtat aaacgtagat gttgaagttg atggtaagat ggaaaaatat ctagtaatgt
    29881 ttaaaaatga aactcacaac catccaactg aaatagaacc atttggtgga gcagctactt
    29941 gtttaggagg agctataaga gacccattat caggaagaag ttatgtttat caagcaatgc
    30001 gtgttacagg aagtgctgac ccaagaacga ctttagaaga tactttacct ggaaaactta
    30061 tgcaaagaaa aataactaca gaagctgctc atggatatag ttcatatggt aaccaaatag
    30121 gtcttacaac tggacaagtt gcagaagtat atgatgaaaa ctttgttgca aagagaatgg
    30181 aaataggtgc agttatagca gcagcaccaa aagagaatgt agtaagagaa agacctgaag
    30241 caggagatgt aatcgttctt cttggaggaa aaacaggaag agatggctgt ggaggagcaa
    30301 ctggttcatc taaagaacat agtgaagagt caatcttaac ttgtagtgca gaagttcaaa
    30361 aaggagatgc tcctaacgaa agaaaaatac aaagattctt tagaaataaa gaagttgctc
    30421 aaatgataaa aagatgtaat gattttggag caggtggagt ttgtgttgct ataggagaga
    30481 ttgctgatag cttagatata aacttagact tagttcctaa aaaatatgat ggacttgatg
    30541 gaactgagct tgcaatttca gagtcacaag agcgtatggc agtagccata aaaaaagaaa
    30601 ataaagataa atttatacaa ttagcagtag aagaaaactt agaagctacg catgtagcta
    30661 cagtaacaga tacaggatat ttaagaatgt tctggaatgg aaaagcaatt gtagatataa
    30721 atagagagtt tttagataca aacggagtaa aacaaactac tgatgttcat gttactaaag
    30781 tagatgaaga aaatacattc tttagctcta atgaaatagt aaaagatgtt aagtgtgcaa
    30841 gtatgaaaga caagtttaca aaagtactat ctgaccttaa tgtttgttct caaaaaggat
    30901 tagtagagat gtttgataat acaattggtg gtaatacagt acttatgcca tttggaggaa
    30961 agtatcaagc gactccaaca caaggtatgg ttgctaagat accagtactt ggaggagaaa
    31021 ctaatacttc tacaataatg acttatggat acaatccaaa ggtaggaaaa tggagcccat
    31081 tccatggtgc cttgtatgca gtagtagaat ctgtgtgtaa attggttgct attggaggaa
    31141 attatagtac aacaagactt acattccaag agtattttga aaaacttggt aacaatccag
    31201 aaaaatgggg taaaccattc tcagcacttt taggagcgtt ttatgcacaa agtaaatttg
    31261 agatacctgc tataggtgga aaagacagta tgtctggtac atttaaagat attgaagttc
    31321 caccaacatt agtttcattt gctgttgaca cagtagatgc gaagaaagta gtatctccag
    31381 aatttaaaaa agcagactca aaagtagtaa tgttatgtgt aaacaaggca gaaaatgatg
    31441 ttgtagattt tgaggaatta aagagaaact tagataaagt aagagaactt atacatggaa
    31501 ataaagtttt atcaacatat gcattaggat ttgcaggtgt aggtgaagca ataagtaaaa
    31561 tggcttttgg taataaaata ggatttaagt ttagtgaaga agctgaaaaa gcatttacag
    31621 ataataaatt atttgaggct agttatggaa atatagtact tgaattagca aatgatgatt
    31681 taagtatgtt agaaggatac aattatgttg tgattggaag tacagttaaa gaagctagta
    31741 tatttatcaa aggtgaagaa cttgcattag atgaattata taaagcacat tgtagtactt
    31801 tagagcctat attcccaact aaaacagaag aagtaaaatc aaagatagaa actataagtt
    31861 atatatcaca aggagaagct aaaaaatcat cattaagtat agctactcca agagtattta
    31921 taccagcatt ccctggaaca aactgtgaat atgactctgc aagagcattt gaaagagcag
    31981 gagctaatgc aagtataaga gtatttaaga atttaactta taaagacata gaagattcta
    32041 tagatacaat agtaaacgaa ataaaatctt ctcaaataat aatgttacca ggtggattta
    32101 gtgctggtga tgagccagat ggttctggta aatttatagc tactgtattt agaaatccta
    32161 gagtgcaaga agctataaat gaatttttaa ctcaaaaaga tggactaatg ttaggtatct
    32221 gtaatggatt ccaagtatta attaaattag ggttagttcc atatggagaa ataagagttc
    32281 caagtgaaag tgctccaaca cttacttaca ataatattgg tcgccatcaa gctaagattg
    32341 ctagaactag aatttcatct aataaatctc cttggttagc tcaaactaat gttggagata
    32401 ttcataatat agcaatttct catggagaag gtaagtttgt tgccagtgaa gatgttatga
    32461 gagaattaat tgctaatgga caagtagcaa ctcaatatgt tgactttaat aatgaagcta
    32521 cttatgatat tgagtttaat ccaaatggtt cattctatgc tgttgaaggt ataactagtg
    32581 ctgatggtag agtgtttggt aaaatgggac attctgaaag aattggtgag gaagtatata
    32641 agaatattat aggtgaaaaa gaccaaaaga tatttgaatc tggagttaaa tattttagat
    32701 aaagctttga tgtttaattg atagctagaa tatataattt agatatttag atttaaagag
    32761 atgtaataat atgatgataa tcatttaata ttacatctct tttttattta tctaagaaat
    32821 aataagttca acctaagtta aaatacaagc tgtgttataa ttaattatta agtaaaattg
    32881 aatatttatt ggagggcaac ataattaatg aaaatattaa taacagggtc taatggtcaa
    32941 ttaggaaaag aattggttaa ccaattagaa gcaatcaatc aaagtataaa ccaaccaaaa
    33001 tatgttatat tagctacaac aagaagtgat ttggacatat ccaatcagac taatgtagat
    33061 aattttattt tgcataataa acctgatgta gttgtaaact gtgcagcata tacaaaggtt
    33121 gatgcatgtg aagataatat cgaaattgca tacaaaataa atgctttagg agtgagaaat
    33181 ctagcaatag catcagaaaa agttaatgct aagcttattc acatatcaac tgactatgta
    33241 ttcaatggtt tttctaaata tccatatagg gaagacaata aaactgaacc caattctgta
    33301 tatggaaaaa gtaaacttat gggagaaaaa tttgttgaac aattctcaca taagtacttt
    33361 atattgagaa cagcatggtt atatggtaat gggaataatt ttgtaaagac catgataaaa
    33421 ctttctttag aaaataaaga ggttaatgtg gttaatgacc agtttggaac tccaacaagt
    33481 acagtagact tagcaaaaat tataataaga atcatggaaa cagaacatta tggagtttat
    33541 catgctacat gtgaaggtga gtgtagttgg tatgattttg ccaaaaagat atttaaacta
    33601 aaaaatatag atataaaagt aaatccaatt aagagtaacg aatttaaatg taaggctcca
    33661 agaccactat attctgtatt ggataatttt atgttgaagt taattggttt aaattctttt
    33721 agaaagtggg aagaatccat tgaagaatat ttagaaagat atgatataaa tgaaaagtaa
    33781 atgtattatt ttgagaaaag aaaataagat attataaaaa atataaatat ttcttttatt
    33841 tatccgataa tcataatatg acaaaaacaa atttaaggat ggaaaaagat gaaaggaatt
    33901 attttagcag gagggtctgg tacaagacta tatcctataa caaaatgtat atcaaaacaa
    33961 acacttccaa tatacgataa accaatgata tattacccta tgtcggttct tatgttggca
    34021 gggatacaag atatattaat aatatctaca ccaagagata taaaagcatt tgaagaatta
    34081 tttggagatg gaagttcact agggcttaac ataacatatg caattcagga gaaagcaaat
    34141 ggaattgcag aagcgtttat aataggagaa gaatttatag gaaatgacag agtgtctctt
    34201 gtattagggg ataatatatt ttatggatat ggatttagtg aaaggttgga gaatgcagtt
    34261 aaaagaaaag gagcaactat atttgggtat catgtaagta atccaaattc gtttggagtt
    34321 gtagaatttg ataaggattt taatgtattg tcaatagaag aaaaacctac agttcctaaa
    34381 tctaattatg ctataccagg attatatttt tatgataatg atgttgtaga gatagcaaag
    34441 ctagttgaac cgtcagatag aggtgaatta gaaataacct ctataaacag tgaatatcta
    34501 aaacgaggaa aactaaaagt ggaacttcta ggaagaggta tggcttggct tgatactggt
    34561 acacataaag gtctattgga tgcttctaat tatgttgaag tggttcaaac aagacaggga
    34621 ctgtatatag catgtttaga agagattgca tatacaaaag gatatataag tagagaacaa
    34681 ttaatgaaac tagccaagcc actgataaaa actgaatatg gtcaatatct attaaaattg
    34741 gctagtgaag atatgtaata tggtagataa aatttattat aaattttatt aagggagttt
    34801 attataatgg gaaaaatcaa tgttataaag actaaaatag aaggattata tatagtcgaa
    34861 ccaaatgtat tttatgatga aagaggatat tttatggaaa gctataataa aaaggatttt
    34921 atagagcagg gtcttgatat gtgttttatt caagataatg agtctagctc aagaaaaggc
    34981 gttttaagag gaatgcattt tcaggttaaa tatcctcaag gaaaattagt cagagttaca
    35041 gagggagaag ttttcgatgt tgcagttgat ttaaggaaag attcacaaac atttgggaaa
    35101 tgggaaggtg taatattgtc tgataagaat aaaaaacaat tttatattcc agaagggttt
    35161 gcacatggat ttttagtttt atcagaaaaa gcagtattta gttacaaatg tacaaattat
    35221 tattacccag aatttgagaa tggctttata tggaatgaca agcaaatatc tattgagtgg
    35281 cctttaaaag gaattgaaga aatattttta tcagaaaagg acaaaaaaca aaaaagtttt
    35341 aaagatattt gtacaagacg ttaataaaaa tttattagaa ttaacattta ggagaaaaaa
    35401 tatgaaaaaa atactgataa ctggaggagc aggatttatt gggagtaatt ttatacatta
    35461 tatgcttaac aagtatgtag attattttat tgttaaccta gacttgttga cttatgctgg
    35521 taatttagag acattaatag aaatacaaga caattccaat tataaatttg ttaaaggaga
    35581 tatttcaaat agggagcagg tgtataagct atttgaagaa gaaaggtttg atatagttgt
    35641 taactttgcc gctgaatctc atgtagatag gtcagttgag aatcctgatt tatttattaa
    35701 aacaaacatt ataggaacac aagtactttt agatgcatct ataaaatatg gagttaaaag
    35761 gtatcatcaa atatctacag acgaagtgta tggtgattta ccaattgata gaaaggattt
    35821 attctttaca gaacaatctt ctatcaatcc aagttctcca tattcagctt ctaaagcatc
    35881 agcagattta ttggtaagtt cttattatag aacatatgga ttgttaacaa ctatatctag
    35941 gtgttccaac aattatgggc catatcattt tcctgaaaaa ttgattcctc ttatgatttt
    36001 aaatgctttg gaaaacaaac aattgccagt atatgggaat ggggaaaatg taagagattg
    36061 gttacatgta tatgaccatt gtactgctat agacttaata atacataaag gaaatatagg
    36121 ggagatttac aatataggag gtcataatga aagaagtaat ctagaagtag taaaaatgat
    36181 attaaatctt ttaggtaaat ctgaagagtt aatatcctat gtaaatgata gacctggtca
    36241 tgatttaaga tatgctattg atgcaactaa aatagagaat gagttaggtt ggaaggcaaa
    36301 atatgacttt gatttgggta taaaagaaac tgttaagtgg tatatagaaa atgaatcttg
    36361 gtggaaagct gttttatcag tataacttta aaatttagaa aggggcgttt tatattgaat
    36421 gagataagtt cactaattaa tatattagca ttgactcaat tatctggtag taatactaca
    36481 aatcaatgta attgtacata taataattca tctggatttg atatgattat gatgaccttg
    36541 ttaaaagcat tatcacaaaa taatcaacat acttctaata gctacaattt attgtctaat
    36601 tctgagaatt tatttaatga acttgataat gtagtaaata ataccactag tagatttata
    36661 gatgttgata caaaagataa aaatgtaaag ttgagaattg aaaatgctgt agatcaagca
    36721 tcaaaaaaat ataacgtaga ttcaaatctt ataaaagcaa taattaaagt tgaatcagat
    36781 tttaatccaa ataccgtttc ttctgctggt gctaagggtc ttatgcaact tatgccagaa
    36841 aactgcagag acttaggagt tacagaccct tttaatatag aacagaatat agatgctgga
    36901 actagacata ttaaggaata cattgatatg tttggaggga gcatagaaat gggattgatg
    36961 gcatataatg gtggtcctgg aagaatgaaa agtagaggag tagaatcaat ctctgacctg
    37021 tataaaatgc caaaagaaac acaaaattat attcctaagg ttatgaaata ttatagaggg
    37081 taggctttat tatgggaata aatatagata agctaaacat aagtccaagt ataaatatgg
    37141 aggttaaaaa actaaaagag agtaataaat tttctgacaa attaaactct ttaaaacaat
    37201 cttcttcatt ggaagatata aaacgaaatt tggaaagtgt aaaaaaggca ggagaaaggc
    37261 ttgtacttac tcaaaactat ggcgatatta gtatatataa aaatgcagta aaagaatacc
    37321 taaaatctgt tgtagataat atgtattcac ttgataaaaa tagtagcttt tgggagcatc
    37381 agtattataa gaatgtaaag attgttgata gtaaacttga ggatatgact agtaagttaa
    37441 ttagtgaaga aaaagaaaac atagatattg tttctactgt tgacatgata caaggcttat
    37501 tgatagatat gtatagataa ggttatacaa ggagaaaaat tttatgaata gcaaaaaaga
    37561 ggatatatat gaattgaatt ttgggcaatt aatagaagaa aaatctggtg ttatagaaga
    37621 aaatcaaaat gtttttaatg caaaagtaaa tgtagctgtt tctataggaa atacaacaga
    37681 aacaataaaa aatatattag atttaaaaga aggctctata ataaaattaa gtaagaatgt
    37741 ggatgaaaaa ttagatatat atgcaaacga tagattgttt gcttatggag aaagcattat
    37801 tacaaatgag aggttatctg taagattatc taaaatagaa gatccagatg taaattagca
    37861 ttttaaatag tatcccttta ggctattctg tatgagttta gctaagggga tatttttata
    37921 aaaaatttaa aatatgtgtg aattatataa atatttttat tttatgagcg ataactaaat
    37981 tataagtatg taattttgga ggtgaaacta tggatattaa aaatgtaagt tcaaatattg
    38041 tagtgaataa ttatttaaag aatacatctg gtataaaaaa agtggaagag gttaaaaaaa
    38101 ttaataaaac agataagata gagttatcta aatttgcaaa ggaaatggca agtgtgtcaa
    38161 atatgagtga tgagaatatt agaaataaaa aagtcgatga aataaaaaat gcattagaaa
    38221 atggaactta caaaataaaa ccaaaagaaa ttgcgaaaaa aatgatagag gatatgcgag
    38281 gataaagtca tgattacaga cttaaaagtt ataatatatg aagaaaaaaa tataattagt
    38341 aagatgatgg aattattaaa agaacaattt gattatatag ttgaaaaaga cttagaaaat
    38401 ttaaatatgt taaatcctaa actagaagat attagcaggg agcttgcatc catagaaata
    38461 aagagaagac aactttttgg tgatgatatt tcaatgagtg aagttgttga aaatagtaat
    38521 gatggatact taaaagaaat atatacagac ttgaaacaca ttcttgcttt agctaaaaat
    38581 caacaagaat caaatgattc tctaataaag aaagaactta tttttacgaa aaaaatgatt
    38641 aatttcataa aaccagctga taagcaaact acaacttata attcttatgg aaatattaga
    38701 aaataaatat taggaggaga aatatggcag gattattttc cattttaaat acagctaatt
    38761 caggaatgaa tgcgcaccaa aaagcaatac agacaatttc tcataatata agtaatctag
    38821 atacagacgg ttattctagg caaaggaccg agtttgccac aaatagtcca atgtatatgc
    38881 ctagtttgag taattcaatt gcaagaggac aattagggac tggagtacat gtaacggatg
    38941 taacaagagc tagaaatagt ttttatgatt accaatttag ggcagaagct cataagtatg
    39001 gtaagacagt ttcaaaatat gattattaca atactataga aactatacta aatgaaccat
    39061 cagactatgg aatatctgca ggtatagatg atttttttaa aggatgggag tcattatcaa
    39121 aggatataaa tagtgacagt aaaaaaagtc tagtggtaga aaacgcatct aatttagcaa
    39181 ctttaataag tgaaagttat aagaagcttg ataagttaaa agaagatata gattctaata
    39241 tagacactga gataaaacaa ataaatgaca tgttaaaaag tcttgaagat ttaaataaat
    39301 ctattgatat tatatctggt tctggctcta caccaaatga tttacttgat gaaagagata
    39361 gaattttaga taacttgagt tttaaactag actttgaaaa tagtgatgtt aaaaatatgc
    39421 ttgcagatgg taaattagaa cttaatgaat taaaaaatcc agatggaact tggaaaacag
    39481 gtatatcagg gaccttacaa ggcttgtttg aaatgcatgg taagattgat acatataaaa
    39541 gcgatttaaa agatgtatct gatggattag ctaaacagat aaatgatgtt tataatacgt
    39601 ctgcaggaat aacagtaaca gattttttta taacttcaaa tgtagatggt gaagatatta
    39661 taaagataaa tccagctata aagagtaacc caaatgaatt aaagcttact gctgatgaag
    39721 cttcaaaaat tgctaagtta aaggatgaaa aaatagatat tggggtggct ggtggaaaag
    39781 taagtacaat aagtgaccac tataaagcct ttgcagaatc aatcggttta gattctcaaa
    39841 aagtaaatca agatgaagca aatcaacgta aaattataaa taatattgat aactcaagaa
    39901 tgagtgtttc aggtgtatct ttggatgaag aaatgacaga gcttataaaa atacaacgtt
    39961 catatcaagc tagtgcaaaa gttatgtcaa cagctgtgca actactggat gttgtaataa
    40021 atggaataat ttaatagaag ggggaattta taatgagagt cagtacaggt atgatgtcta
    40081 gcagctatct aaactcacta caagataatc tacaaagatt ggataaggtt aatagacaag
    40141 ttaatacaac aaaagaaata aacaagctat cagataatcc atataaagca ataaagatat
    40201 taaactcaaa aagtgaaata aagactatgg agacttatgt tgagaattgt aaagatacag
    40261 ggaattggtt agaaactact gatactgcat tagatcagct tggaaaatta ttatcagaca
    40321 taaagaaaac tttggtggca agtggtgatg gtgcatactc agatgatgaa ttaaagactc
    40381 ttagtaatga aactaatgaa aaaatgaaag aaattgcaaa tgctttaaat gcaacacatg
    40441 aagggaagta catattttca ggttcaaata cgggggtacc acctgttgaa tgtatagata
    40501 atgctgatgg ttcagtgagt ttaaagttta attctagtct aaatcttaat aaattaaatg
    40561 actctttgag tatggatata gctcaaggaa taagtgtaga ttataacgtt aaattgagta
    40621 gccttggatt tgacccaaca aaaactgacc catttgaaga gttaaataac atatcaaaaa
    40681 aattagcaaa tccagatgct gatgctataa aagagcttac aacaacttgt ttgggcgata
    40741 tggaacaact aatagaaaat acagtaaatg ttaggtctat atatgggaca aaagcaaata
    40801 cagtagaggc tatgaaggaa aaaaacgatg atagtttgat acagttaaag gatattttgt
    40861 cacaaaatga agaaatagat tatggtgaaa aacttgttca attaaaggca gcagagttaa
    40921 cttatcaagc ttcactacaa acaggaggaa agttatttaa tgtatctata ttagattaca
    40981 tataataaaa taacgcagat atgatgaagg ttacattaaa aaaaggtata ttaggttttg
    41041 aaaatttaaa agaatatgag cttttagata tagaagataa tgattattta aaagaattta
    41101 attctactga agaagaaggt ataggatttg ttgttgtatc tccatttgag attatcaatg
    41161 aatatgaaat agttttaaat caggaaacaa tagaaaaact agaagtgaaa tctccaaagg
    41221 atgttgtatt atttaatata attactcttg gtcagacatt tgaagaatca actgtgaaca
    41281 tgaaagctcc tatagtgata aatataaaaa ctaattttgg aatgcaaata attttacagg
    41341 atgataaata ttcaataagg catccattat tgagaggtga tagtggatgc tagtaatttc
    41401 aagaaaaaaa gatgaagcag tacttatagg agaaagcata gaagtaaaaa ttgtaggggt
    41461 agatggaaac aatgtgaaac tagctatatc agcgcctaac aatataagta tattaagaaa
    41521 agaaatatac gaaaaagtaa agagtgagaa tataaaagct acaaataaaa atataaaaat
    41581 tttaaagtca ttaaaataat aagcttggag gaagtaatat atgtatggag aaaatcctta
    41641 taattcttat aaacaaaatg ctgtttttat ggcatcaaag gaacaattgt tacttatgct
    41701 tgttgatgga gcagtaaaat ataccaaaat agccaaaaca gctatcttag ataaaaatac
    41761 acaaaaagcg catagagagc ttataagagt tcaggatata tttacagaac taatggttac
    41821 attagaccaa aatgcaggtc aatgggcaaa ggatatgtac agagtttatg attttgtaag
    41881 atatgaactt tcaagagcta atattagaaa agacattcaa ataatagata atgtgttgcc
    41941 tgtaatagaa caaattaaag atacttggca tgaagctgat aaaaaaagta aagaagaaag
    42001 aagtaggtac aaataatgga attaaattta gctgaattag ataagttatc aaaagaggaa
    42061 ttactattga tgcttgtaga tggaacagta aaatatacca atatatccaa agaagctctt
    42121 ttaaataacg actatttgaa agcacataat gaacttgtaa gagttcaaaa tatatttaca
    42181 gaactaatga ctacattaga ccaagatgca ggtcaatggg caaaggatat gtataaagtt
    42241 tatgaattta taaaatctga attagcaata gctgatgaaa ataaagatat taaaataatt
    42301 gatgatatat tgccaatagt aaaacaaatt agagatacat ggcatgaagt ttacaatcaa
    42361 ctaaaaatat aattggatgg agggataaag gtatgtcaag tataagtcct ataagagtta
    42421 caggtctttc aggaaatttt gatatggaag gcataatcga agctagtatg attagagaca
    42481 aggaaaaagt tgataaagca aaacaagaac aacaaatcgt taaatggaag caagaaatat
    42541 atagaaatgt tatacaagaa tcaaaagatc tttatgataa atatctaagc gtaaattctc
    42601 ctaatagtat agtaagtgaa aaagcatact cttctacaag aataaccagt tctgatgaaa
    42661 gtattatagt agcaaaaggc tcagctggtg cagaaaaaat aaattatcaa tttgcagttt
    42721 ctcaaatggc tgaaccagca aaatttacta ttaaattaaa ttcaagtgaa cctattgttc
    42781 aacagtttcc tccaaatgct agtggagcga gttctttaac tataggagat gtaaatatac
    42841 caatatctga acaagatact acaagtacta ttgtaagtaa gataaactcc ctttgcgcag
    42901 ataatgatat aaaggcttct tatagtgaga tgacaggtga attgattatt tcaagaaaac
    42961 agactggttc gtcatcagac attaatttaa gagtaattgg aaatgacaat ttagctcagc
    43021 aaattgctaa tgataatggt atcacattta taaatgatgc tggtggaaac aaagtggcaa
    43081 atgtatatgg aaagaatcta gaagctgatg taactgatga acatggaaga gtaactcata
    43141 taagtaaaga acaaaattca tttaatatag ataatattga ctataatgta aattcaaaag
    43201 gaactgcaaa gttgacttct gtcactgata ctgaagaagc tgttaaaaat atgcaagcat
    43261 ttgtggatga ttataataaa ctgatggaca aggtctatgg tttagttact actaaaaaac
    43321 caaaagatta tccgcctctt acagatgccc aaaaagaaga tatgacaact gaagaaatag
    43381 aaaaatggga aaagaaagct aaagaaggta tacttagaaa tgatgatgag ttaagaggtt
    43441 ttgttgaaga tattcaatct gcattttttg gagatggaaa aaatattatt gcattaagaa
    43501 aactaggtat caatgaaagt gaaaattaca ataaaaaagg tcaaatatca tttaatgcag
    43561 atactttttc aaaggctctt atagatgata gtgataaggt atacaaaaca ctagcaggtt
    43621 attcttcgaa ttatgatgat aagggaatgt ttgaaaagct aaaagatatt gtatatgaat
    43681 attctggaag ttcaacttct aaacttccta aaaaagcagg tatagaaaaa actgcttctg
    43741 ctagtgaaaa tgtatattca aaacaaattg cagagcaaga aagaaatata agcaggttag
    43801 ttgaaaaaat gaatgataaa gagaaaagac tttatgctaa atattcagcc ttagaatctt
    43861 tgttgaatca gtattcttcc caaatgaatt atttctcaca agcacagggt aattaattat
    43921 ggataaatta aaagaaaaat taaatttata taaagatata tctttacaaa ttataaattt
    43981 gattgaaaag gaagaatata taaagataag tattaagtta ggtgaaagac aagagattat
    44041 aaatagtgta agtgaaattg atagaaatga ttttatacag ctttataata gaatggaatt
    44101 aattgaaatt gatagcagga taagagatat tttacaagga caattattag aagtaaaaaa
    44161 agaattacat gagtataagc ttactaaaca agtgaacaca atgtattata acttaaatag
    44221 agaaaaagta aatattttta ataaaaaagt ataaagttat gtaccatctt gtccgattat
    44281 ataaatgaag aattaataaa aaagattaaa agtagaaatg acaaggatgt caactatact
    44341 aaggagggta aaataatgag agttaataca aatgtaagtg ctttgatagc aaataaccaa
    44401 atgggaagaa acgtaaatgc acaaagtaag tctatggaga agctatcttc tggtgttaga
    44461 attaagagag ctgctgacga tgctgctggg cttgctatat ctgagaaaat gagagctcaa
    44521 ataaaaggtt tagaccaagc aggaagaaac gttcaagatg gtatatctgt tgtacaaaca
    44581 gcagaaggtg cattagaaga aactggtaac atactacaaa gaatgagaac tttatcagta
    44641 caatcttcaa atgaaactaa tactgctgaa gaaagacaaa agatagctga tgagttacta
    44701 caattaaaag atgaagttga aagaatatca agctcaattg agtttaatgg taagaaatta
    44761 cttgatggaa gttctacaga aataagatta caagttggag caaactttgg aacaaatgtt
    44821 gcaggaacat ctaacaacaa caatgaaata aaagttgctc tagtaaatac ttcaagtata
    44881 atgagtaaag caggtataac aagttctaca atagcaagct taaatgcaga tggaacttct
    44941 ggaacaaatg cagctaaaca aatggtatct agcttagatg tagctcttaa agaattaaat
    45001 acatcaagag caaaattagg ggcacaacaa aatagactag aatcaactca aaacaactta
    45061 aataatacta tagaaaatgt tacagcagct gaatcaagaa taagagatac agatgttgct
    45121 tcagaaatgg ttaacttatc taaaatgaat atattagttc aagcatcaca gtcaatgctt
    45181 gctcaagcta atcaacaacc acaaggagtt ttacaattat taggataaaa taattttgta
    45241 tattggatat agaagaaagg gtaaggcttt gctttactcc tttctttttt tacaaatata
    45301 cttttgaaag gaaaatttat atgttactta gtatagttat gattgttaaa aatgaagaaa
    45361 aaatacttga aaaaacttta aaatcattaa ccacattaag aaattctata gaaagtgaac
    45421 ttataatagt cgatacaggt tctacagatg atactataaa aatatctaag aagtacacgg
    45481 aaaaagtgta ttttcataat tggaataatg atttttctag tatgagaaat atatcaatat
    45541 cctatgctaa aggtgaatgg ttacttatat tagatgcaga tgaaatacta attgattctt
    45601 caactattgt aaaatttttt aaagatggtc ttgataaaaa atttaattct gcttcaatac
    45661 aattaaaaaa tctttactca tatgataaaa aattatatgg atattgttca gtgcttagac
    45721 tttttaaaaa cgttgatttt agataccatg gtaaagtaca tgaacaacca atatacaaaa
    45781 accctatatt taataatgtt gctgattttg aacattatgg atatttgttt gaagatgaag
    45841 aaataagatt aaataaggtg aaaagaaatg aaaaactatt atttcaagag ttaaaagaga
    45901 atgataattc tccatataca aactatcaat tagggaaaaa ttttattata ttaggaaaat
    45961 atcaggaggc tttatactat cttgagaaat cttatgaatt atattctaaa ctagaattgg
    46021 cacctggtta cttgataaca agccttgtaa aaacatattt atatttagga aaacataaga
    46081 agtgtgaaag attgtgctta aagtatatta agaaagacat gaataatatt gatatatatt
    46141 attatattgc acaagcacaa gtagatttag gaaagtatga aaatagtata gattcgtata
    46201 agagatatat ttacttgtta gataattatg aaataagtac acaagcaaat agtttatttt
    46261 cagacactga tactgtaggg cttagagatg aggctattat tacactgata aagatttatt
    46321 ataagcttga aaaatatgag tttgtaattt cagagtataa taacattgaa gatgtggaaa
    46381 agaaaaagaa tgtgtacttt agcttattta tgtcactata taaattgaat agatttgaag
    46441 atataaaaaa ttattataaa gaagtttcaa attctaaaat tgaaaaaaat agtttttata
    46501 gaaatataga agaagttata aataatatta aagaggatga aaaagagtat gtatataaaa
    46561 tcctttgtga cattgaaggt aattatggca ttttaaatca aataaggata aataaatgtg
    46621 tatctataga taaatgtaaa gaaatactca atgaagaaaa ggaaataatt tatgcatata
    46681 ttataaaaat agcttttgaa gagaagattg atttactaga tattttttat gaaatggaat
    46741 atgtatggat agagaaatat ctaaaatatc ttttagcatt agaaagaagt tttaatttag
    46801 tactatataa gtacataatt agtcgtccaa atacatcaga tatggaaaag ataagagtat
    46861 ataaagctat atctaaagta gttttagaaa gtataagctt aagtgatgaa aaatataagg
    46921 agatttttta tctttatata atgtattctt ataagtacat aagatacata tatagcaatt
    46981 taagtgattt tgaattaata aaatttgttt gtaatgattt agataagttc actatagaat
    47041 ttaagcatct acttgatatt aaaaataaag taaataatgc taatgaaaaa ttagagtata
    47101 tacatagtat gaggaaatta ctaaatgagt atccttttta taataaaata ataaaaatac
    47161 ttataggtga attagaagaa aatataaaag aaagtgaaga atttaaggat ttaaaagata
    47221 gttttattat tagtatagaa aatatgattg aaactggcaa aataaatgat gcgaaattat
    47281 tggtagatga ctattcaaaa acatttgtgg atgatataaa gattttaaat ataaagggaa
    47341 ttttatacat gtttgaaaat aagcttgata aagctgattt tatgttaaaa aaggcattat
    47401 cattagatgt agaaaatgaa gatactatat ataatataga atacttaaaa agtttaagat
    47461 aagtcaaaaa taggagaatt gaagaatgaa tataccaata gtggttaatg aaaaatctaa
    47521 agacataata aaagattggc aatatacatg tacaaatgaa gatataaaat ttacaagcat
    47581 aataatattg acatataatc aaatagaata tactaaatta tgtattgaaa gtattaggaa
    47641 atttacccca aagaataaat atgaaataat aataattgat aataactctt ctgatggaac
    47701 agttgagtgg ttaaagggac aatctgatat aaaatctgtg tataataaca aaaacttagg
    47761 atttccaaaa ggatgcaatc aaggtattga gttagctact ggtgaaaaca tattgttgtt
    47821 aaataatgat gtaatagtta ctccaaattg gctctataat ttgaataaag ctttatggtc
    47881 tcaagataat ataggtgctg taggacctat tacgaatgca tgttcatatt atcagcaaat
    47941 tgatgttgat tataagtcaa ttgaggacat gtttatattt gcacacaaaa taaataaatc
    48001 taatattaac ctatggaaat atagattgaa gttagttgga ttttgcatgt taataaaaaa
    48061 aactgttctg gataatgtag gggttttaga tgaattattc acacctggaa attttgaaga
    48121 tgatgattta tcttttagga tgatagagtc aggatataag ttattattat gccaggatac
    48181 ttttatacac cattttggaa gtgtgtcttt taaagaaaat cgtaataaat atacagatat
    48241 aatgtatatt aatagaagaa aatttgaaac taagtgggga tttaatccag aatatagtgg
    48301 aatgataaga acagatttga tttctaaaat agatgatagt ttggaggcaa atattaatgt
    48361 tttagaaata ggatgcggta taggggctac tcttttagaa ataaaaaata tatataaaaa
    48421 tgcatcaata tatggaatag aaattagtga gtcagcaggt aaacttgcaa agtatagtgc
    48481 agatgtactt atagcaaata tagaaaaagt aaaattggat tataaaaaag atttttttga
    48541 ttatataata ttgggagatg tacttgaaca tttgattgac ccatggaaga tagtcaatga
    48601 attgaaacaa tatctaaaga aggatggtta tctggtatct agtataccaa atattatgca
    48661 tgtttctgtt ttaagaaatc ttgtgttagg taattttaca tatgaagatt caggaatttt
    48721 ggacaagact catttaaggt tttttacatt aaatgagata acaaaaatgt ttaatgatag
    48781 taattacata attaatagta ttacagctac tagtatgcca ctttcggaaa ttgataatag
    48841 cttcattgat acaatatgta aggaaagctg taaagaaatg agacaacagt acgaatctta
    48901 tcaatatata gtaaaagcct ctaaaatcct agatactaaa agatattcta gtattagtat
    48961 gattaattta aaatacgctt taatgagaat tgataatgaa ttagatgtag atagtaattt
    49021 aagttatata tttgataatt ataatataac tgaaccatgt tttgttgatg atattgaata
    49081 tctaattgat acatttgtga taaataaagc aactctatta aatagattag gagttgaggc
    49141 atttaacaga ggtctaggag attttagtat aaacatgttt atgacagtat tagagatgga
    49201 tagagatgat attgactcaa tatataatat aacaagtgta ttatgtgaat tagaagaatt
    49261 tgatactgct tataatttaa ttaaaaatag tagcgaaaaa gtacagagtg aaagtgatat
    49321 acaagagata ctttgtttta ttgaggggaa gaaatatgaa taatgataag atatgtttta
    49381 taacctgtgt aaatgatgaa aggctttatg aagaatgtct tgtatatata aatagcctag
    49441 acattcctaa aaactttagt atagaaacag tagctttaag aggagccgat tctattgcct
    49501 ctgcttataa taaagctatg agagaaacag attctaagta taaggtatac ttacatcaag
    49561 atacttttat aattaataaa aatttcatac atgatatttt agagatattt aaaaaaaata
    49621 cagatgtcgg aatgattggt gtagctggag taaaagatat gcctgtgaat ggaatttggt
    49681 gggattctga taaaaaagca gggaaagtat atgatagtca tacaggtaaa atggatattt
    49741 atgaatttag taactttgaa gaatgtttta atgaagttat ggctatagat ggattaatta
    49801 tgattactca atatgatgta ttttggagag aggataagtt tgatgggtgg catttttatg
    49861 atttgtcaca gagtatggag tttagacaaa ataaatacaa agttattgta ccaaaacaaa
    49921 ataagccttg gtgtatgcat gattctggag tagtaaatat aaataatgga tatgatgaat
    49981 ataggataaa atttgtaaaa gagtacataa atcttgattt atatagagaa aatttttatg
    50041 aatttgataa gaagaaagca agtaccatac ttaagagtaa taagcatgta atggattgta
    50101 tgaaagagga gcttacagaa caattaagat taaagaatta caaggatgtt ttaggtattg
    50161 tttctaaaat tgctcatttt tctgttttta atcatactgg tttgtattct tgtgaagaat
    50221 gtgagagagc attgctagaa tgtgcaaata atttaccaga aatagaaaat acgaaattat
    50281 ttacaaaaaa agatttaaaa agaagaaaag tattacatgt tttaagtgaa ggatattcaa
    50341 caggaggtca tacaagacta gttaaaaatt ggattgaaat tgacaaagac tctgttcatt
    50401 cagttgtaac tacatggcaa tcagatacac tgccagactg gttattaaaa gcaataaatg
    50461 atagtggtgg cgaagttata tctttggatt taatttcaga tgattattta aaaagagctg
    50521 tagctttgag gaaaatagct tatgactggg ctgatatagt tattttatat gttcatatgt
    50581 gggatacaat acctgttgtt gcttttggag ttgatgggaa aatacctatt ttacatgtta
    50641 atcactatga tagtactttt tggttaggga caagtattgc agatattgtc attgatttta
    50701 gcgaaactag tcaattaatt acattagata aaagaggcat agataaatct gaaatattac
    50761 caatgccatt aaaagaaaaa gaagtatttt caaaatctga agttagaaaa cgatataatt
    50821 tagaggatga tgtgacaata atattgacta ttgctagtga aagaaaatac aatagtgtaa
    50881 atgatattaa ttactttaaa attgctaaaa cattgattga aaatacagat aatacaatta
    50941 ttattgttgt aggagttgat acagagaatc tctattggaa aactttaagt aatgttacag
    51001 ataatagagt tatatgtttt ggaacaacag caaatataga tgatttttat aagatttcgg
    51061 atatatacct agagtctttc atggtaggaa gtcatacttc aaaattagat gcaattttat
    51121 atgatttaat tcctgttaag tttaaaaatg aagtttcacc aatttttaca aatatgtgga
    51181 attttataga taagtttgat tataagaata taaaagaggt tatagacatg ataagaaggt
    51241 acaatactag tgaatttgac agggataaga taaaagcaaa acaatcggaa ataaaacatt
    51301 ttgttataga taaacattgt attgaaataa aaaactatat agacaaaata tataatgaag
    51361 ttaatcatca taaagttaag tatgatttat ataaaaatga tgaaatcgag gactatcagc
    51421 tttttgtagc attatatggc tatatggtaa aaaataaaaa catgtcatag gtggtaaaat
    51481 gaacaatatt catatcaata aatttgaaaa aagctgtgaa aaaaactggg attattttgt
    51541 agaaaataaa tctataaatg gtacatttct gcaaactaga aatttcctta attatcatga
    51601 ggagggaaaa ttcactgacc actcattaat aatatgtaaa ggtgaagaaa tactagcagt
    51661 cgtacctgca tgtgaaactt atgaagatgg tgaaagaata tttttttctc atttagggag
    51721 tacttttggt ggcattataa taagaagtgg ttttaatgat attaggcatg taaatgccat
    51781 tacaaataag ttagaaatat atttacagga acaaaatttc gataaagtat atttaaaaaa
    51841 tacatcaaga atattttgta aagaggatac ttcattacta gaatattttt tatttaagaa
    51901 taattacatg ttttatgatg aattaagtat gtttttagaa ttaagtaagt ataaagaaga
    51961 tatattagcc aattttaaat acagtaaaag aagagatttt aaatattctt tgaagaataa
    52021 tcttaccttt aaaagactgt atagtgatga agatatagtt tgttttttta atttactttc
    52081 taattgttta gatagtaaag gagctaaagt aatacattca gtagaagaac ttttggagtt
    52141 taaaaataat agattaaaag atattgtaga attttatgca gtatatattg atgacattat
    52201 gatttgtggg agcatggtat ttaactttca agaaagagtt tatcatactc aatatctagc
    52261 atcaaattcg acatataata aatattatcc aatgatattt ttatactata atctaataaa
    52321 agatgcttta gagagaaatt ttaagtatct ttcttttgga attagcactc taaatagagg
    52381 caaatactta gatgagggtt tagctacatt taaagaaggt tttggttcaa gtgcaatttg
    52441 taacagaaca tactataaaa atataaataa gtgagaatca tgttttatag agtttttaga
    52501 gtatagtaaa tatctagtac tataatatta cataaaaata attagtttat aaagaaggtt
    52561 tgtatatgaa aagattaatg attttaggct caattaagta ttttgagaat attgttatgt
    52621 cagctaaaag agagggaata tacacaattg tttgtgataa tagaattgat actcctgcga
    52681 aaaagatttg tgatgaagca atagatgttg atgtatttga ttttgataaa ataaagaata
    52741 tagctataga taaaaagata gatggaattt taactggatt tacagattct ttaatgaagc
    52801 catatgttta tattgcaaat gaactaaatt tgccatgtgt gatttcttgc aatcaacttg
    52861 aatctgtaac taataaggct gttatgaaaa attattttaa aaataatgga atacctacaa
    52921 ctgattattg tattattgaa agtttgaaag atttagaaaa aatacaagga ttaaattttc
    52981 cacttgtaat aaaacctgtt gatggttatg gttcaagagg tgtatatttt gtatcaaaca
    53041 tagaagaatt aatagaaaaa tttgaatatt ctaaggaagc ctcgcaagat ggacaagtaa
    53101 taattgagga attttatgaa agtgaggaaa tacaaggact agcttgggtt cataatggag
    53161 agtcacatgt attttatatt ggagatagag agcttgtaaa tatacataca ggaagagctg
    53221 gaaaaccaga tagattaatt tatccatcaa agtattgttt tcaatatgaa gaagaaataa
    53281 aatccatata tcaaaatata acgcaggctt ttggcataaa aaatggacct ctttatgtac
    53341 aaatgttagt gggagcatat ggaataaagg tatctgaagt aatgccaaga ttacctggag
    53401 gttgtgacta tatggctatt tctcaaataa cagatttaga cataggaagt ttatttgcaa
    53461 actttagtgt gaataatgaa gtaaattttg atgaaataaa gaaatataat atgcatctat
    53521 caaaatgtgt atatgcatta ccaatataca taagacctgg cacaataaaa aagataaata
    53581 atatagataa aatagaaaac ttagattatg tcactcaatt tctattacta gtaaaagaag
    53641 gagatacaat aggattatat gaagatgaga gacaggattg tggaagagtt tatggagtag
    53701 caaataatgt atttgatgca aatataaaga agaaatgtat tcatgaaatg atagaaatac
    53761 ttgatgaaaa taatgaaaat atgatagaaa atttttaaat aagggagtat gatagatatg
    53821 aagctaataa ctcatgaaca aattatgtca ttaaacattt ctcagaaaca aggatacgag
    53881 tggataaaag aatccttttt aacaaaaaaa gattgtcaat taccaccaaa aataagcatg
    53941 gtagaagaag ggcatgtatt ttacaatgta atgccgtccc taaatcataa tcataagatt
    54001 ggtggaataa aaatggttac aagattgcca gggagaaatc caagtttaaa ttcagagata
    54061 tttctatata atatggaaga tgctaaatta aaggctattt tagatggcaa ttatattaca
    54121 gcttttagga ctgctgctgt tgctgccttg gctatcgata catttgctaa aaaagatttt
    54181 aatactattg ctattatggg tttaggtgta actgcttctg ctataatgaa tatgctttca
    54241 tatactctta aagataaaaa tataaatata aagttactta aatataaaga ccaagcagag
    54301 tcgtttgtaa aaaagtatga aaaaaatgaa caatttagtt ttgaaatatg tgacacacat
    54361 gaagaactta ttgtagattc agatgtagtt atttctactg tgacatatac agatgaatat
    54421 ttatcagaat ttaaatggtt taaaaaaggt gctttattat tacctgtaca tgttatggga
    54481 tttggaaatg ttgattatct atttgataaa gtatatttgg atgattataa tcatttgaaa
    54541 catctagaaa atatagatag atataaatat tcttgtgaat tttatgatgt tttaagtaaa
    54601 caagctaaag gaagagaaaa tgatgatgag agaataatat cttacaatat aggtttgtca
    54661 atacatgata tattatttgc aaataaaatt tacgaattgt gtgaaacact aagctgtgaa
    54721 gaaatagaat taaaagaacc aaaagaaaaa tattggatat agtttatttc attcatattt
    54781 acaatagttt attgtaaata tgaatatgaa gtaatatatt gttagaaaat tagcatgaat
    54841 aggatagata aatttatgga taaatataat ttaattaggt tcgtatctat tggaaacgaa
    54901 tatataggaa atttagttgc attagaatca aataaagcaa taccttttga tataaagaga
    54961 gtttattaca tatatgatgt accaaacaat gtaaagagag gatttcatgc acacaaaaac
    55021 ttggaacaag tactagtttg cctaaatgga agtgtgaaga taaaatgtta tgatggtgta
    55081 aaagagacta tatttaattt acataaaaat gatgttggat tatatatagg aacgggagtg
    55141 tggcatgaga tgtttgattt tgatgaaaaa actgttttaa tttctatagc atctgaacat
    55201 tataatgaag aagattatat tagagactat aaagaatttt taaagtattt aaataatgtg
    55261 tagttattat acaattagtg atgtattttc ttaattaaat aaatttaaga tagccacata
    55321 gagaatagat agattaggat gtgaatttat tgttggttaa atttaataat ttattaaaac
    55381 agttccaaac tcataaagaa gaatatgaac agaagattaa ggatgtaatg gaaagtggat
    55441 attatatcct tggaaatgaa gtaaagcatt ttgaagaaga atttgctaac tatgtaggta
    55501 gcaaatattg tgttggttta gccagtggtt tagatgcact gataattgct tttagagcat
    55561 taggaataag caaaggtgat gaagtaatcg tgcaatcaaa tacatatatt gctagtgtta
    55621 tgggaatcag tataaatgga gcaacaccag tatttgtaga accaaatgaa tattataata
    55681 tagattcatc taagatagaa gaaaagataa atagcaaaac aaaagctata ttagtagtgc
    55741 atttatatgg tcaagcaagt aatatgaaag atatacaaga attatgtgat aagtataact
    55801 tgtatttaat tgaagattgt gctcaatctc atggagctaa atatgatgga aaaatgacag
    55861 gaacatttgg tgatataggt tgttttagtt tttatccaag taaaaatttg ggtgcatttg
    55921 gagatgcagg agctatagta actagtaatg aggaaatagc tgataaagtg aaaattctta
    55981 gaaactatgg ctcagaaaag cgatattata ataaatttgt cggatataat tcaagattgg
    56041 acgaaattca ggcaggttta ttaagagtaa aacttaaata tctaaatgaa attgaaattg
    56101 aaaaatcaaa aatcgtagat agatatctta atgaaattaa taatcccaaa gtcatacttc
    56161 caaagataga aaataattgt actcatgtat ggcatttatt tgttttacaa gttgaaaaac
    56221 gagatacatt tatggaatac ttaaagaaaa gtggtataga gtctataata cattatccga
    56281 taccaccaca tctatctgaa gcatatgctt atttgggata taaaaagggg agttttaata
    56341 tagctgagaa ctattcagat aaagttgtta gtataccttt atttattggt atggaagatg
    56401 aagaagttag ttatataatt aaaattataa atgaatatta aactactcat aataatatca
    56461 aaaataaatt ttttatatat catttattta aatttcgtta attgacattt ttaaatattg
    56521 atatattgta caaataaaat tgaaatatat ggaaaaatat agaattaaga tgagatttgt
    56581 cttatatttt tataaattaa aaatattttc aaagagttta ttataaaatt gtaaactata
    56641 gtgtttataa tctttgtaat tattggtaat taaatttaaa aaataaaata tttaattata
    56701 cacaaaaaag atatttaaaa aatttgttat ttattgtata cttaagttaa aactaaatag
    56761 gcaaatctag agaaatctag tgacgcaaag ctatagggac taaggtttat atacataaac
    56821 tatgtcagcc agttgccaaa aagagtccta ggtgtattgt atacctaaga aaagtctata
    56881 atgactgcct ttttggcagt cattttgtta tggaaaaatc actttatttc agtagaaaat
    56941 acacataata acatatttta ccaattataa cataaaataa agaaaaatta tctcaaaaag
    57001 ttactatttt acaaaaaatg acttctttat ttatttaaat taatcgcatt tccattacat
    57061 taatacaata atgtatcaat ttagtaagaa aactaatatt tttttaatga agtcccatga
    57121 aaatataaaa aaattttaat atgtatttaa atatttcttc gaaaagttgc tctttttgta
    57181 atatagcaac ttcatttttt ttattaataa acaaaataga taaaatatat tttgaggagg
    57241 ggtgagtgtg aatacatatg acattataaa aatgggatta gatgcaacta atctaagaag
    57301 tcagacaata gcaaataata tagcaaatat aaatactgaa ggatataaaa gacattatat
    57361 aacttttgaa gaaacactaa atgatgctat gcctaaaata gaagaaaaaa cagacaattc
    57421 taaaggttta agagtagatg gtaataatgt agacatagaa gaagaaaaag taaatcaagc
    57481 aatggcaaca ttacaatata atgctttgat aagttttgca aatggaaaaa tagcaatgac
    57541 caaatcaata ataagtggga ggtaattaga agatgagtgt gttcagtgga atgagaatat
    57601 ctgctagtgg attaacagca gaaagtttga aaatggatat catatcatcc aatgtagcta
    57661 atataaatgc tacaagaggt ccaaatggag aagcatatag gagaaaagtt gtttcatttg
    57721 aagaaaacta tgataataaa ttaggtatgc taggagtaaa agttacatct gttgaagatg
    57781 ataaatcacc tcttaaagaa gtatatgacc catctcatcc agatgcaaag ccaaatggat
    57841 atgtagaata tccaaatgta aatatattgg tagaaatgac agatttaata acagcttcac
    57901 gagcatatga ttctaacgta gatacattga atgctcagaa gtctatgata tcaaaagctt
    57961 tggaaatagg aaagtaggga taaattatgc caattacaca aattaacaat ttaatgcaag
    58021 gtgcaaagtt agataatttg attggagaag caaagccatt aactcttaat aataaagaag
    58081 aaaaaaagaa ttttgaagat gtaataatag atactataga taaagttaat tttcaacaag
    58141 taacagcaga tgatatgaaa gaaaaattta taaaaggcga agatgtttct atgcatgaag
    58201 ttatgttaat aggtcaagaa gcacaactat ctatgcaata tttaatagaa gttagaaata
    58261 agattcatga tgcatatcaa gaaatgaata aggtgcaaat ttagcctttt gaacaggagt
    58321 aataatcaat gataaccagt tttaaaaata agattaagga gcaagttgct aaatctagga
    58381 caaagataag ttctttaaaa aagggaacta aaatagcatt aggtataggg gttattgcta
    58441 tatgtttagc gctagtattt acagcaatat ctgcgagcaa aaataagtat gcagtcttat
    58501 tttcagaact agatgctcaa gatgctgcag atatatcagc agagctagag tctaaaaaga
    58561 tagatactaa ggtaaaagga agtacgattt atgtacctag aacagaagtt gataagttaa
    58621 ggctggagtt agcgccaagt cttacaggtg gttctaaagg atttgcacta atggatgata
    58681 gttcatcaat agggcttact gatgaagaat ttcagattaa gaaacaaaga atgatacaag
    58741 gtgaaataga aaaaacaata aagagttttc cacaagtgga agacactaga gtacatataa
    58801 catttggaga gtcatcagta tttgagaagg aggctgttcc agggagtgca gcagtttatt
    58861 tgatgctaaa accagggacg actttagaaa aaaaccaaat aaagtctgtt atggcactta
    58921 tatcaggaag cgttacaaat ataccagaga aaaatgttca ggttattgat aacaaaatga
    58981 atttattatc tgaaggtatt ttggaggatg aaaatgttga tgaaaataat caaccaataa
    59041 atagtggcat tagtgttgat aaaaataagg catctgaaca agcattgaat aaacaactag
    59101 aaaagtctat tttagaaatg ctagaaccga tatttggtaa gggaaaggtg aaagctacag
    59161 taaatgctga tttaagtttt gatacagctg aaaaaactga gataaaaatt gaccctgata
    59221 aagttgcaat aaaggagaca aggtcgaaaa attcatctaa atctgctgca aataatgttc
    59281 aaggtgtaga tgctaacatg aataaccaag gaaataacaa tggtactaat acagaagata
    59341 gtttagatga gagttttgag tatgaaacag gtaagattga aactcacact atagttgcgc
    59401 ctggagaact taatagattg acagcttctg ttgcaataga tggaaaaata gctaaaggtg
    59461 ttcaagctga tgtagaggat attgttaata atgctatagg tatggatgga gcgaggggag
    59521 atactcttag cgttgtttca atggtatttg acccagaagg taagaaagag gctaaacaag
    59581 agctggaaga gattaaaaaa gaaggattta aaaagaacat aataaaagca gttattggag
    59641 tagtagcagt aatagcagtt ggagctattg gatacttaat atataagaaa agaagtgaac
    59701 gagaagatat ggattatgaa gatgaagatt atatttctaa atctgattct gttgatagga
    59761 aggataatcc tctattagga gatgatccag aactgacatt agaggaagct gttaaaatgt
    59821 atgcagaaga aaagccagac caagtaacag agataataaa gacttggtta aatgagtaag
    59881 ggggatagaa agtgtccacg gaaattacaa atgttaatgc gtttgactta agtgatataa
    59941 atgaaagtga taagaatgag aaggtagaag gtgagggttt aaaaaaagct gctgtattat
    60001 taatgacatt aggcccaaat gtgtctagtg atataataaa aagcttgcct gataaacaag
    60061 ttcaaaaaat aggtttggaa attgcaaata tttcttcaat agcttcaaaa gaaagaagag
    60121 aaatcttaaa agagtttgta gaaataaata gggcaaaaga ttatgttata gaaggtgggc
    60181 ttgaatatgc aagagagtta ttaagtaatg ctttaggaaa tgttaaagca aacaaattac
    60241 ttgaaggtat atcaataaat acaagtacaa agccttttaa acatattata aaactagatg
    60301 taaagaagat attagaaata cttaggaatg aaagtgcaca aactatagcc gttttacttg
    60361 cacatatgga accagaaaat ggagcagaag ttttatcata cttagatttt gatttacaaa
    60421 atgaagtagc tatgagaata agctcaatgg gttcaatatc tccagaagta ataaaggtaa
    60481 ttgaaagttc attagaagat aaattacatt caataagtgg tgatgatttt aatgattatg
    60541 gaggggtgtc taccttagtt caaatattga ttaattctga tagaaaaaca gaaaagaata
    60601 ttttgggaac tattgaagaa caaaacccgg atttagcagc tcaaattaaa tctcatatat
    60661 ttgtctttga agatattgtc aagttagatg atataagcat acaaaaagtg ttaaaagaag
    60721 tgactatgaa agatttagta tatgccttaa aaggtgtaga tgatgagatt tcagatgcta
    60781 tatatagaaa tcaatcttct agagcagcag atgcacttaa agaagaaatg gaaatggttg
    60841 gtgttataaa ggttacacaa gttgaggctt ctcaacaaaa gatagctaat atcgtaagaa
    60901 aacttgaaag agaaggtgcc ataactattg taaggaattt tggtggtgac tttgttgaat
    60961 aataagatta ttaaaagtaa aaaagcatta tataaaggag tgctagattt aaccataata
    61021 gataaagtta gagtgggaga atcaaatgta aataaagaaa atgattgtag atttagggat
    61081 gaggtaataa aagaagctat agaatctgct gagataaaaa agaatgagat attagaagaa
    61141 gcaaaaataa atgcacaaaa tatagaaaag caagcatatg aaaaagggta tagtcaaggt
    61201 caaaaaaatg gatatgagga tggatataaa gagtcatatg atgaatatac ccaaaaagct
    61261 aaagatgaag cagattttat aaaaaatcag gcaaatttaa tcttattaca agctaatgag
    61321 aagatgactg agtacataaa agaaaaaaat aaagagattg taagacttgc tattaatatg
    61381 gccagttctg ttttaaaaaa gcatttcgaa gatgaagaat ctatgagtga tctgttaaag
    61441 caaattattc aagattatga aggaagtagt tcatttatag taagatgtaa ttctttctat
    61501 aaagataata tagagaaaga gtttgaatta ttcaaagagg aaacatcact aaaatcaagt
    61561 gtatttgtta ttgcagatga tagtatagat aaaggcaatg ctattatcca aaaggaaaat
    61621 ggaagaataa tagttggtat tgattgtgct atggaaaaac taaaagaaga attgcttggt
    61681 gaataaagta tgtataaaac tgatatatct atagattttg acaaaataaa taagaaaata
    61741 gaaaatttag aactcactat atcagaaggt agagtaaaga agataatagg acttactgta
    61801 gaagttgaag gaataaaagc ttttgttggg gagttatgcg ttatatacaa ccaagttaat
    61861 aaaccagtga attgtgaagt agtaggtttt aaagataaag aagtcatatt aatggcgttg
    61921 ggagaattaa cattgatagc acctggttgt aaagttattt ctaaggggat acctttaagt
    61981 gttatgtgta gtgataattt actaggtaaa gtacttgatg gtcttggaaa tcctatagac
    62041 aactcagatg ctgttttagg agatagatac aacctgaaca atgaacctcc tgatccaatg
    62101 aagagaaaaa agatacgaaa tataatggaa acaggtgtaa gagcaataga tgcatttact
    62161 acttgtggtg aaggacagag aatcggcata tttgcaggaa gtggagtagg taagagtacg
    62221 actctaggta tgattgctag gaatgcaaaa gcagatgtaa atgtaattgc tcttattgga
    62281 gaaagaggaa gagaagtact agattttatt gataaagatt taggtgaaga gggaatgaaa
    62341 aaatctgttg ttgtgtgtgc aacatcagat aaagcccctc tagttaggtt aaaaggtgct
    62401 cttactgcta ctgcaatagc agaatatttt agagaccaag gcaaaaaagt aatacttatg
    62461 atggattcag taactagatt tgctatggca caaagagaag ttggtgtagc aataggagag
    62521 ccaccagctc aaaaaggata tacaccatct gtatttgcaa tgttgccaaa acttatggag
    62581 agaacaggga catcagataa aggttctata actgcatttt atacagtatt agttgatgga
    62641 gatgacttta atgagccaat agcagataca acaagaggga tacttgatgg tcatatagtt
    62701 ttatctagag acttggcaaa taagaatcac tatccttcta tagatgtatt aaacagttta
    62761 agtaggctta tgaatgaaat tgcttcaaaa gaagacataa aaatagcgtc ttttgcaaga
    62821 gacatgcttg ctgagtatag agaagcagaa gatttgataa atataggtgc atatgcttca
    62881 ggtacaaata aaaaaataga tgaagcaatc tattatcatg agcatattat taatttttta
    62941 aaacaaggga taaatgaaaa gagttctttt aatgaaacta taagtagttt gagaagagtt
    63001 tttgaataaa caattaaaaa aatatttgaa taggttgtga tggaattgga taaaaacttt
    63061 aagtttagat tacagaaagt tttagactta aaagtgaaag atgaagaaga gataaaaatg
    63121 gaatttgcta agattcaaca aaaaaaaata gatatagaga ctaatttaga aaacttagaa
    63181 agtaattata gcaaatattc tataagtaaa aataacgata gtattcaaaa tcaaaaaata
    63241 actattaact atttactagc tttaaacaat tctataatgg atttatcaga agagttggat
    63301 aagtcaacaa atgaacttga aaaagctaga aagcagctta taagcaaaca gatagagaga
    63361 aaatcattag aaaaattaaa agaaaaaaag tatggacagt attataaaga agaaaattta
    63421 aaagaacaaa acactaatga tgagtttgca agtatgagtt atttaaggaa tagacaagtt
    63481 ctataagaat agtaggaagt gaggatatta ttgtatgaag atacaagcag attcttataa
    63541 tattattaaa gatattgggg atagaaaaag ttcatctaag aacaaaaata taaaaaataa
    63601 taaatttgaa gataatctat ataaggcaac agaccataaa aaagttaaag atagaaggga
    63661 agtaagtaag caaaatatta gctcagataa accggaaaat agtgataatg agtgtcattt
    63721 agatgaagat aaaagtaatc acttagaaga tgaaaataca tatgaaaaag attgtagtaa
    63781 gaataatgaa gctttgcagc aattactgaa tcttttaaag gataattata aaaaagataa
    63841 agatataaac actatattga ataatataga aaaactaagt atatcagatg aattaaaaca
    63901 agaacttaag cttaataaaa atatattaaa taagattgca aatcaaaatt cagagctaaa
    63961 taaagctaat ataaataact ctaatgatat agaaaattta gacatagtta aattaagaga
    64021 acttttggaa gataatgcta ataaagatat tgaagtaatt actaaaacta cagaaaatga
    64081 taccaaaaat atagatttgt atagttttga tagcaataga atggataatc ttaactcaaa
    64141 taaacagaaa gatgatagct ctgatatttt ggaaaaacta gcaggggtaa atggaaataa
    64201 gagtgggaat tttggacaag taataaataa aaatgttgat atgaataaaa ttaaaaatga
    64261 taatgtcaat ttaaaacaga tagaatcaaa tgtaatggat gactctataa aagctattaa
    64321 gcatatgaaa acaaatgata tacaagaatt gacaattaaa ttaagaccaa aagaattagg
    64381 agatatgaat atacaattgt taaaagatgg tgaaagtatg agagctgttg tgactgtttt
    64441 caataaagat gtctttgact ctataaataa aaacatagca gatttaaagc aacatcttga
    64501 acttactaat gtaaatataa aagatgtatc agtgcaaatg cacagcgata atacaaatac
    64561 atctgatacc tttgataaag catttgaaca acagaataga caaaataacc aagaaaacat
    64621 gagtaagaag aatacaaatg ataagcataa taatgtagtc atagaagatg acattaaaga
    64681 aaatactatt gatgatgata gagtagattt actagcttag taagggggaa gtattgcatg
    64741 tacggaatag caacggcttt ggcaggaact cagggaaact ataggtcaaa taacttaagc
    64801 caacttgaaa gaaatattaa tgatattgcc aataaacaag ttagaagcaa aggaaatcag
    64861 acagtaacag acaatgggac tccaattata ggacaaggtg atgatgctga taaagacttg
    64921 tttatgaaga tacttgtggc acagatgggt aatcaagacc caatgaatcc gcaagaccca
    64981 acagaatatg tgtcacaatt agcacagttt gcaactttag aaaagatgtc agctatgaat
    65041 gagaagctag atgtactttt agccttaagt aattccactc taataaactc tgctttaagt
    65101 atggcaacat cattaattgg taaagaagta gaagtatctg gggaagagaa agaagatgaa
    65161 aacattgtag gagtagtaaa gtccacatat ataaaagatg gggaagtaca cttagaatta
    65221 gaagtagatg gaagtgacaa aactgtaggt gttaaatatg aaaaattact taaaatagga
    65281 gaggtagatg aaaattcagg agaagaaaat gattcttcag aacacgaatc tccagaagaa
    65341 gcataatatt aaatgagaaa agaggtaaat tcatgataaa agcaatgtat tcaggtgtaa
    65401 gtggaatgaa agcaaatcaa acaaaattag atgtaatagg taacaatgta gccaacgctg
    65461 gaactacttc ttttaagaaa agttcagcta gaataacaga ttcattttat caaactgtac
    65521 tatatgcaag tgctccaaca gctgcattag gaggaacaaa cttaggacaa gtaggtgtag
    65581 gtagtaagat atcaagtata aataaagata tggtacaagg aaatgtacaa ccaactaata
    65641 gaccatctga tttaatgata gatggagatg gatatcttcc agtagtaaga caaggaacag
    65701 ttatgtatac gagagatggt tcttttaatt tagatacagg atttaaagca gctgatggtg
    65761 ctttgaagaa tattaaaggt ggaagacttg taaatggtga tggatatatg ttacaaggta
    65821 tgatttatga tggggaatat aaagctgata ctggagaatt tgttaaaaag ggtgatgcta
    65881 aaggaaaaga gcctattgta ataccacttc aaagtataag tcctaaggat ggaacagttc
    65941 aaaatgtact agagtataat atatcaaagg atggaacagt agaattttta ttgagtgatg
    66001 gtcaaagaac tcaatttgtc tatgatatac ctgatggtgc taaggtagga gatacacctg
    66061 ctggaataga taaattaaaa ggtacaatac aaaaagtgca aacatatgca ttccaaaatc
    66121 cagcaggact agatgcagaa ggaggaaact tgtttaaacc atcagcaaac tcaggagacc
    66181 cacaaatagc aggagctaca ggcacaataa tacaaggggc aatagaagct tccaatgtag
    66241 atatagcaga agaatttaca gaaatgataa tagcaagtag gtctttccaa gcaaacagta
    66301 aaataatatc gacatcagac gagatacttc aagaaataat aaatcttaag agatagtagg
    66361 tacatattat gataagagta acggatttaa acggaagaaa atacatatta aattcagacc
    66421 ttatagtgag gattgattct gtaccagaaa gtaggattct ccttacaaca ggagaaaaaa
    66481 accttgtaaa agagtcggta gatgagatag tagataaagt aataaagtat aaaagaaaaa
    66541 taaatgtagg ttatattagg agcgaaaaat aatgaaaaaa acagattcac tgacatttat
    66601 tggactaata gtatctacag tattagtttt agttggtgcc gcaaaaggaa gtagttcagg
    66661 gttaaagaat ttttttgatg tttcatcaat attaatcaca gtgcttggtt catttggagc
    66721 tctaatgatt acatttacta tagatgatat aaaacttata aaaaatgcat tacaatactc
    66781 atttaagaca atgagtgtat caaagttaga tttactagaa caatttaaaa cactttctaa
    66841 aaaagctaga aaagaaggtc ttttatcaat agagagtgat gtaatgggaa tagaagatcc
    66901 atttatgaag aaagggttag agctgtgtat tgatggattg gaaatcaatg agataaggtc
    66961 catcttagaa ctagagatag attctgttga agatacaaat aacagatact ctaaactatt
    67021 taaaacttgg ggaacctttg cacctgcttt tgggatgttg ggaacactaa taggtttaat
    67081 acaaatgtta gctgacctta catctccaga tttaatagca tctggcatgg gaaaggcatt
    67141 aattacaacg ttttatggtt ctttacttgc caatatcgct ttgaacccaa ttgcatataa
    67201 tatagatgag aagactgaaa aagaaattta tgtaaaggaa atgatgttgg aaggtataat
    67261 aagcatacaa tctggggaga gcagtatagt agttgaggaa cgtttagcca catacctttc
    67321 aaacaatgag aagttagaaa ttatgaaatc caataaaaat acagaaagag caatgagcaa
    67381 tggcgcgtaa aaaaaataag aaaaaggatg agataaatcc agatgcatgg ctggctactt
    67441 atgcagatac tataacactg attcttacat ttttcgtatt attgtatgca acatcagttg
    67501 tagatatgga aaaatttaaa aacatagcag atgcgttaaa aggtcagttt tctgggacaa
    67561 gtatatttag tacgggttca agtacaagtg agaaatctcc aatagacagt gttttagtgg
    67621 ataaaaatga taaatcaaat ttagaacagg atttaaaaga taaagttaat aattctaact
    67681 tacaaaatag tgtaacagtc aaagatgata gtagagggat tttactagag ttagatgatt
    67741 caattttatt tgactctggg atttctgagt taaaagtaga tagtaaagct actcttaata
    67801 aagtatatga catgataaag atgatgaaaa ataagatagt tatagaaggc catactgata
    67861 atgtacctgt aaaatcaagt acacatgaat ccaattggga tttatcttct agtagagcag
    67921 taagtgttgt aagatacttt gtagaagaaa agggcatgga ccctagacta ttttcggcaa
    67981 caggatgtgg agagtatcaa cctttggttt caaatgaaac agcaaaggga agggctgaga
    68041 atagaagagt taatatactt ataactattg actaggagga agaggtttgt tcaaaaataa
    68101 aaaaattatt ataattattt tgatactgat tattttctca gcgggagcag gtttttttat
    68161 attaggagaa ggtggaaata aacaagttga caagattgca gatttgttta ttccaacagg
    68221 aaaaggtaaa gatgaagtaa agaccgaaaa tcttgactta gaagagatgg tattaaagct
    68281 agctgatgaa ggtgtaagat atttaaaagt atcaataact gtatcttatg attcttcata
    68341 taaagatata gaaaaaaata cgcctttaat tagggataat attataaatt gttttatggc
    68401 aaaaagggca gatgatttta ctgctgataa tttaaagaac ataaaagaag aaataaaaaa
    68461 tacattaaat atacaactag gtgaaaatgt aattcaaaat atatatttca caaatatagt
    68521 agtgcaataa aaggatggta agacatgata ctttatttac taaaaatatt aatatgtatc
    68581 ccaataattt taatattgat agtcttatct ttaaaaatta gccaaggaac tttattaaaa
    68641 aaatataatg ataaatatgt aaaaatacta gatattgtaa atataagtaa aaacaatagc
    68701 ataataattt taaaaatagg tgaagaagga tgcattatgg catcttcttc ttctagcatg
    68761 agtaaaataa aagatttgac caaagaagaa atattaaaaa tagaaaacga cattaaagaa
    68821 agaaatttta aaattcaaga ttttaaaatg gaaagaatta aaaagaaata tgatttcagt
    68881 agatatattg gaaaatttac aaaaaaggaa gataagtaaa tggaactatt atcaaaatta
    68941 cctttggctg atgtaccata tacaagttca atgcaattgt ttttatttct aactgcatta
    69001 atgtttttac catttgtaat gttcatgatg acaagctttg tcagaatagt tataagttta
    69061 tcatttttga aatcagcatt aggagcacag caagcaattc caagtcaaat tttggtagga
    69121 cttgcaatag tattgacaat atttataatg agaccagtat taaatgagat aaacgaaaag
    69181 gcattacaac catatatgaa agaagaagtt actatggaag aagctatgaa ggaagcagaa
    69241 ggacctatta aagagttttt attaacacaa accagacaga cagatttaga tttatttgta
    69301 gaacaggctg gtttaaaaga aaagaaatta actcgtgaaa atataccact atctgttgta
    69361 gttccagcat ttgctataag cgaactaaaa actgcttttc aaataggttt tttaatatat
    69421 ataccatttt tgataataga ccttgttgtg gcaagtgttt tgatgtctat gggtatgttt
    69481 atgttgcctc cagtcatgat atcattacca tttaagttat tattatttgt aatggtggat
    69541 ggaacttgat agtaaaaaca ttgatattgg gatttggata ggagaaaaat agtatgagtg
    69601 aatttatgat aatgagtgtt cttaaaaatg gcatgtttac aggagctatg attgcagctc
    69661 cagtactcat tgtttctctt gtagttgggc ttttaataag tgtgtttcaa gctacaactc
    69721 aaatccagga gcaaactttg acttttgtac caaaactaat agctattctt ttagtagtag
    69781 ctattggtgg aagctggatg ttacatactc ttattagttt tacttctgag atattcaata
    69841 tgatatctct tataactaaa taagtaggat acatatgatt tttgtattta ttagaatagt
    69901 agcattcttt ggaacactaa gaatactatt tccagctggt acacctgctt tatttaagtc
    69961 actctttgca atcattatat caatattgat ttcaagtact atgcaaattg aatatgcaac
    70021 aaatatagac aacattgtat tgtttacctt atatggtgta aatgagacca taactgggat
    70081 tatgcttgga tatataacca acctatgttt ttatagtatc agaatggcgg gttctatgat
    70141 ggaccaacag atgggattat caatgattaa tatgtttgac ccaaattcaa tgactcagac
    70201 aactttaatt gataatctta tgaactggac agcacttatg atatttttta gcatggatgg
    70261 tcatcatgtt ttaattagag gtataaggta tagttttgag ttaattccaa taggaaaacc
    70321 atttgtagat aataacatag attatataat aaacatattt gtgcagtgct ttttaactgg
    70381 attcaaaata gcaataccta ttgtactttg tctgcttatg gcagatttta ttttagggct
    70441 tatatcaaga agtgtacctc aattaaatgt aatgatagtt ggtatgccat taaaaatatt
    70501 ggttggtatt gcattattta tcatatcaat acctttaatt gtcaatcaaa taagtcattt
    70561 attaagtcaa ataccaaaga tgtatgaggg aacttttgca ctagctccta tgttttttat
    70621 gggttcagca gataaaacag aagaagctac tccaaagaaa aagggagaac aaagaaagaa
    70681 aggtaatata gcaaagagta gagaattacc cgttgcaatg actctacttg cattcacctt
    70741 attagtacct acattatttt catatgtagt agatacactt aaatcttcgc ttaattattt
    70801 tttgagtcta gatttttata tgaacataaa ttattcaaac ctagagaaat tagttatagc
    70861 aggattaatg gattttttta agatattttt accaatagct attccctttt tagttttagg
    70921 cataatagca aacctatttc aggtgggaat actgtttact ggtgaaactt taaaaccaaa
    70981 tttatcaaaa ttaaatccaa ttagtggatt caaaaacatg ttttctatga gaagtttgtc
    71041 aaccttaata aaggatacag cgattatatc tattttggct tacataggat atacattctt
    71101 ccaagataat tatttagata tattaaagct aggtaacata tacctaccaa ctttaatgta
    71161 cacagttaaa gacttagtat attcaatatt gagtaagatt tgtgtagcta tgatagctat
    71221 agcagttgca gattatgttt atcaaagata cagtcataaa aagcaactga gaatgacaaa
    71281 acaagaagtt aaagatgaat ataaaaactc agaaggagac ccagaagtaa aagctaagat
    71341 aaaacaaaaa caaagacaaa tttcgtctca aaggacaatg caagcagtac caagtgctac
    71401 tgtaatagta accaatccaa cccacttatc tatagcagtg agatatgaaa aagggaaaga
    71461 ccaagctcca gtagtggtgg caaaaggtgc agattatcta gcttttaaaa taagggaaat
    71521 tgcaaaggga aataacatcc ctataataga aaacaaacct attgctaggt tgttatataa
    71581 gcaagtagaa atagaccaag aaattccaga ggacatgtat caagcttttg cagagatatt
    71641 agtggcagta tataaaatca aaaatagata caaagtcccc aaaaggtagg ataagtaatg
    71701 aataagttta atttaaagga aaatatggat gtaatagtgg catttggtgt tgtagggatt
    71761 gttcttatga tgatagtgcc attacctaca tttatattag atattttact ggcaattaac
    71821 ataagtttat ctgtgctaat tttactaatg acattattta caacaaatgt acttgattta
    71881 tctatatttc caacagtgtt acttgtgaca acactattta gattgggatt aaatctttca
    71941 tcaactagat tgattttaac acagggatat gcaggtgaag ttatagaggc atttggaagt
    72001 tttgttgtag gtggaaatta cgtagtagga ataattatat tcttaattat attaattgtt
    72061 caatttgttg ttataaccaa tggttctgga agagtatctg aagtaacagc tagatttacg
    72121 ttagatgcca tgccaggtaa gcagatgagt atagatgcag atttaaatgc aggagttata
    72181 gatgacaaaa ctgctagaaa aagaagaaaa gagcttcaac aagaagcaga tttttatggt
    72241 tctatggatg gagcaaataa gtttgtaaag ggagatgcca tagcaggtct tatagtaaca
    72301 gcaataaaca taataggtgg aattgtaata ggagctgtaa tgctgaatat gactcttatg
    72361 ggtgcagcac agacatatgt aagacttact ataggggatg gtcttgtaag tcagatacct
    72421 gctctattaa tatctacatc agcaggtata atcgtaacaa gagcttctac tgatgaaaac
    72481 tttggtgact tagtaggtaa acagttaacg gcaataccaa aagcagtaat gatgactggt
    72541 tgtgttctaa tcgtacttgg tatgatgcct ggattaccta aaccagcatt tttttcacta
    72601 ggtataggag ctatagcagc aggttacttt ttaggaagag aaaagaaaga tactttagaa
    72661 gacttggagt ttgaaaaacc tgatatggta aacccagata aaaatgaagt agaagatgta
    72721 acaaatttaa taaatgtaga atcaatagaa gtagaaattg gatatggttt aatatcctta
    72781 gctgatgaat cagcaggagg agatttactt cagagaatag catcaataag aagacaatgt
    72841 gcaatagata tgggtatagt cgtacaaccc ataagaataa gagataactt acagttaaat
    72901 ccaaaccaat acaatataaa aataaaagga aatgtagttg gtgggtatga gataatgcct
    72961 aatatggttt tatgtatgga ccctatgaat caaggattta gtatatttgg aataaagaca
    73021 attgaaccta cttttggaat agaggcactt tggatagaaa gtgacaaggt agaagatgca
    73081 gagttaaagg gttatacagt tgtagattca tccacaatac ttataactca cttactagat
    73141 ataataaaat caaatgcaca cgaattactg ggaagacaag aagttaagac tattatagat
    73201 gcagcaagag aaaattatag tgctgtagta gatgaattga taccagattt gatgacactt
    73261 ggtgaaattc aaaaagtact taaaaatctg ttaagagaaa aagtaaatat aaaggataga
    73321 gttacaatac ttgaaacgct tgcagaccag tctagaaata caaaagatat agagcttctt
    73381 actgaatatg taagaatagc cttggctaga agtatttgta ataatttagt tgatgatgat
    73441 aagacaattg tagtggctac attgtctcca gaaactgaag agttggtatc taataattta
    73501 cagaggtctg taaatggaac ttatccagca gttgacccag aaaatacaaa taaaatattt
    73561 gaaagtatac aagaagtaat gagcaatgta tatttcaatc ataatatacc agtaatagta
    73621 gtatcaccaa agataagagc tccatttaga aaattagttg aaattgtata tcctaatttg
    73681 actgtattat cattaaatga aatacctaac gatattaaaa tcaaagcaga aggtgtggtg
    73741 agcatatatg atgacgaaaa agtatacagc taacacaatt caggatgcta tgaatctagc
    73801 caaaatggaa ttgggtgaca atattacatt aatagataaa aaagaggtaa gaaagtctgg
    73861 aatacaaggt atcttttcaa aaaaagatat tgaactaact attggatggg aaaagagaga
    73921 aaatgtagaa caaaaagatt taaaacgaga gatagaacaa ttaaagtcaa ttattaacaa
    73981 tatggggttt gataataaga atgataatga tatagataaa atatgtaaaa atctactaag
    74041 tttagaattg aatgaagaaa ttgtagaatc tataagaatt gatttgcaag aaatgaagtt
    74101 caatggaatc gatacaagta aaaatttagt agaaatttta aagaaaaaga ttaaaataga
    74161 aaatcaagta ataaatggta aaattgctct agtcggacca ccaggagtag gtaaaacgac
    74221 tacaatagca aagttagccg caaaattagt ttttgaagaa aataaaaaag taggagtaat
    74281 aactatagat acatacagga ttggtgctgt agaacagttg aagatatata ctgatataat
    74341 gaatattccc tttaaagggg taataagtcc tgatgaaatg gaattggcac ttgacgagat
    74401 gaaagattgt gatgttgtat taatagatac aacaggtaga ggctacaaaa actctatgca
    74461 aatactagaa atcaaaaatc ttatagataa agctgaaact gataatatac atttagtttt
    74521 gaattgtaca acaagagaaa gtgatacaaa agcaattata gattcatata gaaatgtgaa
    74581 ttttaaaagc ttaataatta caaagctaga tgaaacaata acatatggct caatatttaa
    74641 cataatgaat tatgcgcaaa aaccaatatc atatattaca acaggacaaa atgttcctga
    74701 tgatattata aaacctaatg aagataagat tataaggtta ctattaggag ttgaaagtat
    74761 atgatagacc aagctcaaat tttgagaaaa atagcaatgg aaaaaagagg acttgatgag
    74821 tttatagtag agaatgagaa tgtacctaag ataattacaa tagcttctgg taaaggcggt
    74881 gttggaaaga gcaatctcgc tacaaatttg tctatatgct tgaccaaact taataaaaaa
    74941 gttcttatat tagatgcaga cataggaatg tctaatatag atataataat gggggtaaat
    75001 gtaaaaggga ctataattga tgtaattaat ggcgaaaaaa atatagaaga tataatttca
    75061 cagactaagt atggtgtaaa tataatatct ggaggttctg cccttaatca tatagaagac
    75121 tttacagaag ctcaaagaaa taagtttata catagcatag aacaaattca cgatgtggat
    75181 tttataataa ttgatacagg agctggtatg agcaaaagcc tattgtcatt tatatactgt
    75241 agtactgaat ttttcttaat aacaactcca gaaccgacat cattgactga cgcatatagt
    75301 ttattaaaag ctataagtaa ttttggcata aagagaagtg caaatataat aatcaatagg
    75361 gtaattgatt tagaagaagc aaaatctaca tacaaaagaa taaaaatggt tgtggacaaa
    75421 tttctaaacc ttaatctaga attgtatgga tatataatag atgacaaaaa agtaagttta
    75481 tgtgtaaaaa aacagatacc attcatttta gaatatccta caagtattgc atcaaaatgt
    75541 attatagcca tagcaaagag aatggtaagt cagtcaagag aagatattgt tgataataaa
    75601 agttttctaa gaaagatgtt tagtatattt aaaagctaag gaggcgtagt taatgaatag
    75661 agaagaatta ataaaagaga atatgcctct tgtaaagagt atagcaagaa aattttttat
    75721 accagggaaa ggttttgaat atcaagactt agtaaatagt ggagtcattg gattgattga
    75781 tgcaataaat aaatttgatg ctgaaaaagg ggcaaagttt tctagttact cttatataaa
    75841 aataaaatct gctatattag atgaaattag aaatcaaagt ccaatttcta aacataatct
    75901 aactaaagta aataagtata atagagtggt agaaaagttg caatctaaat tattaagaga
    75961 gccaacttca tatgaaattg caaaagaatt aaaggtttca gaaaaagaac ttcatgatat
    76021 cgaaagtaat atagatatgt taaatattgt atcattaaac tatgtaatct ttgaagatac
    76081 aaatgaaact gtacaagatg taataagtga tagagaagag gaagctccag aaaatataat
    76141 agaagaagaa gaaaagttag agattctatc caaagcaatt agtaatttaa atgaaagaga
    76201 aaaattaata ctttcactgt actattatga agatttaaat ctaaaggaga tagggaaagt
    76261 gctaggtgtt tcagaatcaa gagtttcaca actccacaga aaatcaataa gaaacttaag
    76321 aaacaagata aaagaactta aatattctat atagatggtg attgataaga tgatacaaat
    76381 tatattaata gtagtgtcta ttttaataat agtaactata gttataaatt tacataagga
    76441 atcaaaaaat aaagataaca gatactttga taaaatattt gagcaagaat atgaagcaaa
    76501 tgatagatta ataattgcag aaaaaagaag attcttttta gacaaagtac ttgcagaaaa
    76561 aagaattaat agtcatataa aagtttatga aaattataac ctaataaagg ctaatgaaga
    76621 aagaaaggaa aacatattta tagaatcaaa aaaggaagta agacaagaaa atgagttatc
    76681 tcttaaagtg aaacatctta taagttcagg ttatgatgat gtggaaatat gtaagatgct
    76741 agatattgga aagggggaac tgttattaat aaggagttgt tacaaaatat aaggatatta
    76801 ttatgtgata aaaaagtatt cttaggaata ggtattggta tattaatatc aactttatgt
    76861 ataatgcttt ttagtaataa tgatatgagt aaggcagaga tagaaaaaaa ggccagagcg
    76921 ttaggcatga aatatacaag tgagatgaaa gttttagaat agatgatatt gtttagacat
    76981 ttacaaaaaa ggaggtcata atgtgataag aggattatat acagcagttt ctgctatgat
    77041 aactaatcaa caaaagcaaa atgttgttgt caataaccta gctaatatgg atacaaatgg
    77101 atataaaagt aaacaacttt tgactaaaag ttttgatgaa ttaaagttgg agggttacaa
    77161 taactacgca aatggaacta aacaaaagca gattataggc gatataagtc caggtatttc
    77221 tatggatgaa actatcacaa attttaatca aggacctata aaaactactg ataataaaat
    77281 ggatgtagct attcaaggta agggattttt tctagttagt gatgcagctg gaaatcaatt
    77341 ttacactaga aatgggaact tcagaacaga caatcaaggt gaccttataa caagtgaagg
    77401 gtattatgta cttgggacga atactgctac tggtgctgta ggtcatataa atgtaggaaa
    77461 tcaaaaattt gaagtggcaa aagataatac aatatcacta gacggaaatg ctatgtataa
    77521 atttaatatt gtagatttta ataactacaa taacttaaaa aaagaaggag ataacttata
    77581 ttctggagga ggagctatga aagtaaataa tgcacttaca atacaaagtg cgatagagtc
    77641 ctctaatgta gatatggtat ctgaaattaa taatatgatg acaatatcaa gagaatatga
    77701 ggcaaatcaa aagataattc aggctatgga ttctaaatta gcaaaaatag caagtgagat
    77761 tggttcagta agataggtaa taggaggtga atttttatgt acacaatgtt ttatactagt
    77821 aaaacagctt tatcagcaaa tcaaagtaaa ctagatataa tatctaacaa tatagcaaat
    77881 tctgattcaa caggatataa aaaaataaat atggaatatt ctgatttagt ggatgaagta
    77941 ctaaatagac catcatatcc tacaaatggc aatgatatat cgactggaac aggagctaag
    78001 gcaacagctc ccatgagaaa ttatgaacaa ggtgcattgg ctcctacaga tagtaagagt
    78061 aatttggcaa tagatggaga aggattcttt agagtgataa gaaaagatgg tacatatgca
    78121 tatacaagaa atggtggatt taatgtagat gctttaggta agattgtaga tgataatgga
    78181 aatatattag atgtccaatt tgatgcagga tataactata ataatacaaa tatagatagt
    78241 acaaacctga cagtttcaag aaatggagag ctattcgtag ataataaaaa aataggaaaa
    78301 atcaacttat atcaacctat aggaactcaa aattttatat ctgaaggaga tagtttattc
    78361 gtcgcaactg atgcagcaca gattaaacaa gttgagaaag tcgatatagt tcaaggctat
    78421 agagagaggt caaatgtttc tttacaagaa gaatttgttg acttaatagc cactcaaaga
    78481 gcatttcaaa tgaactcaaa gggaataaag acagcagatg aaatgtggca aatagcgaat
    78541 aatttaagag ctaaataatt atgtaaggtg aggaatattc atgaatgata tgaaagagta
    78601 cgattttggc aaacctcaga gttattcaag agaaaattta acttgtttaa attttttatt
    78661 agctgaattt tgtaaaaaat ataaaaacta tataatttat gaacttaagt gtaattctag
    78721 tatggttgtt gatagtatag accaagtaaa ttatcagact tttttagata gagtaacttc
    78781 ttcatgcgta gtagtacaaa atgcaatgga acctgttatg aaaaactttt cttttagaat
    78841 agatagaagt gtagctgata tgtggattga tataacttct ggaggaactg gagttgtaaa
    78901 ggataatgat agagaactta cagaacttga taaaaaagtg cttttacacc ttatggatga
    78961 tttagtaaga aatatgcact tatttgaggg ttttgaaaat atacaagtat tggatacaca
    79021 tacacatatt aatttgccac aattatgttc tccaactgca ccagtatgcg ttgtagattt
    79081 aaaagtgcta aatgataatg agtatattgg aagtgtttct ctttgctttc catacaatgg
    79141 tttagaggct ctgttagaaa gtatatctgt tatagaattt tttgataatg atgtaggaga
    79201 tgattcagag gaatttaccc aaaaaatcta taatagtgta tctaatatag aattatcagt
    79261 tattgcagag ataggaaaag tatcaattaa tgttgaggat ttactcaaac tagaagttgg
    79321 agatgtaata gtaacaaata aaaaaataaa tgattatata gatatatttg tagaaaattc
    79381 aaaatcatac acagcaacac caggatttat atcaagtaaa aaaggtgtaa aaataaatga
    79441 tgctgttgga aaagaggtgt agttatggac aatagtaatc ttatttcaga tgaagaagca
    79501 aagatgttat taaatgataa agttgaaagt gaagttgatg gagatgatga gttagcctta
    79561 gatgtctctg aagttaatcg aaataatata caaaggatac ttgaccttaa gttagaatta
    79621 tctgtagtta taggaagaac taagatgact ctaagagata ttttaaattt acataaaggt
    79681 tctcttattg aactggatac tttggcagac caagatgtgg aaatactaat agataataag
    79741 gttttggctt atggaaaagt agtggtggta gatttaaatt ttggtgtaaa gataacaagt
    79801 atagtaagtg aagaagacat ggtaaaatct cttgtttaga gataacatca attaatactt
    79861 aaggcatgag gtggtactta ttttaaactt aaatgatata aaaaaagata tactaaaact
    79921 caataaaaaa gactcatgtt ctctatataa tgaatctatt aataatataa ataataattg
    79981 tatatcaact ggattaaaat ttttaaaaca agcaagagat ttaaacccag atgatgttga
    80041 tatattaaat ctcacaggtc ttgtaaactt actaaaatgt aattttgatg atgctgtaga
    80101 aagtttctat aagagttcac tttgtgaaaa aactgctctg tgtgaaaagt atataaacat
    80161 gcttacatct gaggagtttt taattttctt tgaaaagtac aatcaactta taaggttaat
    80221 aaataaagga atgtataaag aagctataca agggtttaaa cttataactg accaatactg
    80281 tgatttaata gaaccttatg agttgcttgc cttactgtat gacaaggaag aagaatatat
    80341 aaaatttgat gaatgtttag aagtattgaa gattattgat aaggaaaatt atttattaaa
    80401 tcaagaaaat aacacattat atatgtataa tagtgctaat atacaaacta aaagtaatat
    80461 aaaagtagat gattataaga atgagttctt acataaaagt agtagtaacc ataaaattaa
    80521 tgattataat gatgtaacta aagaaaagag ttcaagcaaa aaccagaaaa gttcagattc
    80581 taataagaaa aaaagtatta aaaaaaataa gtacctatat atagccataa tattaggaat
    80641 attattagta ggacaaagta tatatagttc ttataagatg aataatttaa ctgataaaat
    80701 atctaaaaat aatgaaacta aaaatgaaga gtcaatagat tctaacttga ctaagaaaga
    80761 aaataatata gaagaaaatg aagatatgag taaggcttct aatataaata agagtaataa
    80821 aaacattaat aaagaggatg atgtaaaatc taaaaaaaat actgtagaaa atgaagatga
    80881 taaagattct gacttattta cagaggatga attgatgtct aaggcgaata ctttaaaatc
    80941 agaaaagaaa actaaatctt ctatacaact atataagaaa gttgcagata taggtgaaaa
    81001 taaagggaat acatctgaag caacttatca agttgctatt ctcagtgaaa agttaaaaga
    81061 ctataaaact gctgaagaat attataaaat gtatgtagaa aattactcag aaaaagatgc
    81121 ttattttgat gaatcttatt ataatttagg tatgatgtac tataacaatg gtgatttaaa
    81181 aaattctaaa ttgacattaa agaagttggt taataaggta ccaaatagta tgtataataa
    81241 ttcaaaagta aaagaaattt taaaagaaga atagttaaat tagataagaa aaataaatta
    81301 ttttatttta aaataatatg attataatat attgacatac cattttaaaa aatttaaaat
    81361 attaattata aaatgaggag ggataaaaaa tggaatttga aaaagggagt atttcaattc
    81421 atacagaaaa tatttttcct ataattaaaa aatggttata ctctgataaa gacatattta
    81481 taagagagct tataagtaat ggatgtgatg cagtaagtaa acataaaaga cttgtatcac
    81541 taggagaaat atcagaaaat aagtcatcag attataaaat tacagtatct gtaaataaag
    81601 gagaaggtac acttaaattt attgacaatg gaattggtat gactgaagaa gagataaaaa
    81661 aatacataaa tcaagttgct ttttcaggtg ctgaagattt ctttaataaa tataaggata
    81721 aaatggaaga gtcaaatgat ataataggtc attttggatt aggattttat tctgcattta
    81781 tggtgtctaa aaaggtacaa atagatacac tttcatatac agaaggagcg actcctgtaa
    81841 gatggatatc tgaaggtggt acagaatacg aaatatctga atcagatgct agaaatgata
    81901 gaggaactac tataacacta tttattgatg atgatagtaa agaattttta gatgaattta
    81961 ctgtaagagg aataataaat aaatactgtt ctttcttacc agtagaaata tatttagaag
    82021 atgttgaaag attagaaaga gaagctaaag aagcagaaga aaaagctaaa aaacaaaagg
    82081 aagatggaaa agaagagatt gtagatgcaa aagtaattga gcctttaaac gacactaatc
    82141 ctctatggtt aaaatctcca aaagactgta ctgatgagga gtataaagag ttttatagaa
    82201 aggtattcaa tgtatttgat gaacctttat tttggataca tttaaatgta gattatcctt
    82261 tcaatttaaa gggtatactt tacttcccta aattaaaaaa tgagtttgaa ctgactgaag
    82321 gaaaagtaaa attatacaac aatcaagtgt ttgtggctga caatataaaa gaagttatac
    82381 cagaattttt acttttatta aaaggagtta tagactgccc agacttacca ttaaatgtat
    82441 caagaagttt cttacaaaat gacagggatg taagcaaaat ttctaaacat ataattaaaa
    82501 aagtagctga taagttgaaa tctctttgta aaaatgaaag agaagaatac aataagtttt
    82561 gggatgatat acaaatattt attaaatatg gttgcttaaa agatgaaagt ttctatgaga
    82621 aggtaaaaga atgtatatta ttcaaaacta ttgatgatga gtatataaca ttacaagatt
    82681 atttagaaaa atgtaaggac aaacatgaaa ataaggtatt ttatgtaagt gataaggaac
    82741 agcaatctca atacattaaa ctatttaaag aatacgactt aagtgctgtt gttttaaact
    82801 catcaataga tactcatttt atttcattta tggaatataa agagaatgga gttaagttta
    82861 atagaataga tgcagacctt tctgatgtat taaaagataa aaatgaaaat aaagatagtg
    82921 aagaaaacaa agaagagata gctaagatag aaggtctgtt taaagaagct gtcggagaaa
    82981 gagtgaagaa ttattctgta gaaggattga aaaatgaaga cactccagct atggtattag
    83041 tatcagaaca atctattaga atggcagaaa tgcagtcaag atttgctgga atggatttag
    83101 gaatgaattt tgaggaagaa aaaactcttg taataaatga gaacagtcca ataatcaaaa
    83161 aattggtttc tcttaaagat gatgaggaaa agaaagataa gattacgctt atttgtaacc
    83221 agatagcaga tttagcttta ctttctaata aagaattaaa gccagatgag ttagattcat
    83281 ttgtacaaag aagtaataag cttatgagtc ttttgataga attataagat tgaatttgaa
    83341 tacattgaag caaatttata aaaagtattc aatatcagtg ataagaaaga agtgtctcaa
    83401 aatgaacttt tcagttcatg aggcgctttt tttatgtgaa attaaattta ttttaatcat
    83461 ttcaacaatg aaaaatatat ttatctctat tttgagacag accattcttt atttttttat
    83521 ctttggttaa ttttaaatat ttaagtaggt tctaataaaa aactctattt agttaaatat
    83581 agttaacttg atatataata agataataca tataattaag taaaagattg ttttttagtt
    83641 tatgtttaaa tacataatta cactttgtta aaaacataaa attattacta aagtagaaat
    83701 aggaggtttt agaatgaatt ttaattataa tttgccagta aatatcttat ttgggagagg
    83761 cagaattaat gatgtgggaa aagaagtatc taaatatggc aaaaaagtat tgatagtaac
    83821 aggtaaaaat agtacaaaga aaactggatt acttgataaa acaatagatt tactaaaaga
    83881 ctctaaaata gaatatgaag tttttgatag agtagaacaa aatcctctta taactactat
    83941 atattcagga gtagaaatta taaaatcaac tggatgtgat tgtgttttag gtcttggtgg
    84001 tggaagcatt atggatgctg ccaagagtat agctttttct tataaaaacc caggagattt
    84061 aaatgattat atatttggaa ttagacaagg tgaagaagct ttacctataa tcttagttcc
    84121 aacaacatca ggtacaggta gtgaaggaaa ttgttttgca gtacttacaa attcagaaac
    84181 taaagataaa aaatctataa agaagaatag tatgtatgca aaagcatcaa taattgaccc
    84241 agaacttatg gttacaatgc caaaacatat aattgcatca gttggatttg atgcacttgc
    84301 ccataatatg gaagcatatc tttctaatgg aagaaaccct cttgcagatg tacaagctat
    84361 ttatgggatt gaacttatat cagaaaacct aataaaggta tataatgatg ttaatgattt
    84421 agaagcatgg gaaaaaataa cacttgcaag tactattgga ggaatgacga ttggtacagc
    84481 gggtacagcc ttgccacatg gaatggagca tcctgtaagt ggtcttagag atgtaatgca
    84541 tggaaaagga cttgcagctt tgactcctac tattatagaa agttcatggg atagtgatat
    84601 tgaaagattt ggtaatattt caaaggtatt aggaggaact tgtgcagaag attgttcaga
    84661 tgcaattaga aatttcctta aaaaaataga tttagatata aaactaagtg atttaggtat
    84721 tgaaaaatca gatatagatt ggttaactga taattgtatg aaaatatcag ttgcaaatat
    84781 taagagacat ccaaagtatt ttaataagga acaaataaag gaaatgtacc ataagtcttt
    84841 ataatttgac tttacatatt tagttttata taaaaattat aaattaataa taaaaaatta
    84901 taagtaatta atagtaaaaa aa
//
LOCUS       DAMJVI010000005        77337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.005, whole genome shotgun sequence.
ACCESSION   DAMJVI010000005 DAMJVI010000000
VERSION     DAMJVI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 77337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.005"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(121..894)
                     /locus_tag="PC545_000420"
     CDS             complement(121..894)
                     /locus_tag="PC545_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HDF2455216.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(894..1280)
                     /locus_tag="PC545_000421"
     CDS             complement(894..1280)
                     /locus_tag="PC545_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2455217.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDVFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1305..1622)
                     /locus_tag="PC545_000422"
     CDS             complement(1305..1622)
                     /locus_tag="PC545_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDF2455218.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHALIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     VEG"
     gene            complement(1634..1894)
                     /locus_tag="PC545_000423"
     CDS             complement(1634..1894)
                     /locus_tag="PC545_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDF2455219.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1894..2190)
                     /locus_tag="PC545_000424"
     CDS             complement(1894..2190)
                     /locus_tag="PC545_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2455220.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC545_000425"
     CDS             complement(2259..4685)
                     /gene="gyrA"
                     /locus_tag="PC545_000425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDF2455221.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFKLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC545_000426"
     CDS             complement(4705..6606)
                     /gene="gyrB"
                     /locus_tag="PC545_000426"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDF2455222.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKRYKQTYEKGLPTSKLEVVGESQ
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPSTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPSKSEIFLVEGDSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC545_000427"
     CDS             complement(6607..7722)
                     /gene="recF"
                     /locus_tag="PC545_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDF2455223.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRINKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRAFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKNIHVDEALLDVYDDTLAKYGSYIYILRRDFIKKIANISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEVSHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC545_000428"
     CDS             complement(7739..7945)
                     /gene="yaaA"
                     /locus_tag="PC545_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDF2455224.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(8082..9188)
                     /locus_tag="PC545_000429"
     CDS             complement(8082..9188)
                     /locus_tag="PC545_000429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDF2455225.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC545_000430"
     CDS             complement(9430..10752)
                     /gene="dnaA"
                     /locus_tag="PC545_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDF2455226.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11408..11545
                     /gene="rpmH"
                     /locus_tag="PC545_000431"
     CDS             11408..11545
                     /gene="rpmH"
                     /locus_tag="PC545_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDF2455227.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11623..11967
                     /gene="rnpA"
                     /locus_tag="PC545_000432"
     CDS             11623..11967
                     /gene="rnpA"
                     /locus_tag="PC545_000432"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDF2455228.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEAYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11999..12250
                     /gene="yidD"
                     /locus_tag="PC545_000433"
     CDS             11999..12250
                     /gene="yidD"
                     /locus_tag="PC545_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDF2455229.1"
                     /translation="MNKIIYILKEISKHLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFVKGMYLTIRRLLKCHPFHPGGYDPLK"
     gene            12282..12989
                     /locus_tag="PC545_000434"
     CDS             12282..12989
                     /locus_tag="PC545_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2455230.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYVLAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12958..13587
                     /locus_tag="PC545_000435"
     CDS             12958..13587
                     /locus_tag="PC545_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HDF2455231.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKTKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13699..15078
                     /gene="mnmE"
                     /locus_tag="PC545_000436"
     CDS             13699..15078
                     /gene="mnmE"
                     /locus_tag="PC545_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDF2455232.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGEKALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDNENVIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKEKTDELKKDIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVEKSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15176..17068
                     /gene="mnmG"
                     /locus_tag="PC545_000437"
     CDS             15176..17068
                     /gene="mnmG"
                     /locus_tag="PC545_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDF2455233.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKKQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQIRRT
                     RGGKGE"
     gene            17061..17780
                     /gene="rsmG"
                     /locus_tag="PC545_000438"
     CDS             17061..17780
                     /gene="rsmG"
                     /locus_tag="PC545_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDF2455234.1"
                     /translation="MNNIELLKNGIEGFNIDTNDSMLEKFKIYREILVEWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDSLELTLLDS
                     LNKRINFLEEVSRVLELDNITFIHGRAEDFGKNEDYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEVDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17943..18728
                     /gene="noc"
                     /locus_tag="PC545_000439"
     CDS             17943..18728
                     /gene="noc"
                     /locus_tag="PC545_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HDF2455235.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKVQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18863..19636
                     /locus_tag="PC545_000440"
     CDS             18863..19636
                     /locus_tag="PC545_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDF2455236.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19638..20501
                     /locus_tag="PC545_000441"
     CDS             19638..20501
                     /locus_tag="PC545_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDF2455237.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETSEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLSDSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20534..21676
                     /locus_tag="PC545_000442"
     CDS             20534..21676
                     /locus_tag="PC545_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2455238.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKALEKRGIENTVVKCDYEGFLDYEDLEKSIKNNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYDIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRASVIALNIGDMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21810..22385)
                     /locus_tag="PC545_000443"
     CDS             complement(21810..22385)
                     /locus_tag="PC545_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HDF2455239.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGSDSSKKETKSNTNVEQ
                     PKEENNTEKEVPNNKAKPTPKEETKAQSATIYSFDVDKTDLIENKVDLNKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAETLMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22645..23937
                     /locus_tag="PC545_000444"
     CDS             22645..23937
                     /locus_tag="PC545_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2455240.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSESKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEVTLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            24042..24644
                     /gene="yedF"
                     /locus_tag="PC545_000445"
     CDS             24042..24644
                     /gene="yedF"
                     /locus_tag="PC545_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDF2455241.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDNLEEGVVVTIVDNETAKENI
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNVVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24717..24980
                     /locus_tag="PC545_000446"
     CDS             24717..24980
                     /locus_tag="PC545_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDF2455242.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILVENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24995..25180
                     /locus_tag="PC545_000447"
     CDS             24995..25180
                     /locus_tag="PC545_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDF2455243.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25283..26479
                     /locus_tag="PC545_000448"
     CDS             25283..26479
                     /locus_tag="PC545_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2455244.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDSDGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26693..26971
                     /locus_tag="PC545_000449"
     CDS             26693..26971
                     /locus_tag="PC545_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDF2455245.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            27008..27442
                     /gene="ssb"
                     /locus_tag="PC545_000450"
     CDS             27008..27442
                     /gene="ssb"
                     /locus_tag="PC545_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2455246.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27461..27688
                     /locus_tag="PC545_000451"
     CDS             27461..27688
                     /locus_tag="PC545_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDF2455247.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27831..28151
                     /locus_tag="PC545_000452"
     CDS             27831..28151
                     /locus_tag="PC545_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2455248.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28159..29115
                     /locus_tag="PC545_000453"
     CDS             28159..29115
                     /locus_tag="PC545_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HDF2455249.1"
                     /translation="MNNKIRLSKAMSIIVLAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFADISYALNICIMYSLPGIVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNSFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSKLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29126..31120
                     /locus_tag="PC545_000454"
     CDS             29126..31120
                     /locus_tag="PC545_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDF2455250.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRRHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31121..31570
                     /locus_tag="PC545_000455"
     CDS             31121..31570
                     /locus_tag="PC545_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDF2455251.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRSLGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31589..32917
                     /gene="dnaB"
                     /locus_tag="PC545_000456"
     CDS             31589..32917
                     /gene="dnaB"
                     /locus_tag="PC545_000456"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDF2455252.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33122..33430
                     /locus_tag="PC545_000457"
     CDS             33122..33430
                     /locus_tag="PC545_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455253.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKYEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33512..34801
                     /locus_tag="PC545_000458"
     CDS             33512..34801
                     /locus_tag="PC545_000458"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDF2455254.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKTDNISTENIKISDRA
                     HVIFPYHKELDALSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAVRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35127..35202
                     /locus_tag="PC545_000459"
     tRNA            35127..35202
                     /locus_tag="PC545_000459"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35160..35162,aa:Lys,seq:ttt)
     gene            35209..35283
                     /locus_tag="PC545_000460"
     tRNA            35209..35283
                     /locus_tag="PC545_000460"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35242..35244,aa:Glu,seq:ttc)
     gene            35288..35363
                     /locus_tag="PC545_000461"
     tRNA            35288..35363
                     /locus_tag="PC545_000461"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35321..35323,aa:Val,seq:tac)
     gene            35397..35471
                     /locus_tag="PC545_000462"
     tRNA            35397..35471
                     /locus_tag="PC545_000462"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35429..35431,aa:Thr,seq:tgt)
     gene            complement(35854..36846)
                     /locus_tag="PC545_000463"
     CDS             complement(35854..36846)
                     /locus_tag="PC545_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2455255.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSIDDE
                     ISKIGLSLAKIVLLNPKSKDEIVKKTKENIESLKVKKERLLAESNIPLDYLEIKFQCI
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKGSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDTNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36855..37913)
                     /locus_tag="PC545_000464"
     CDS             complement(36855..37913)
                     /locus_tag="PC545_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDF2455256.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNNSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNEEYDNFNYSIEFLDLK
                     NFYIENILSNNSSIKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIINACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            38038..39501
                     /locus_tag="PC545_000465"
     CDS             38038..39501
                     /locus_tag="PC545_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HDF2455257.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNVDYINNTKSNIDKIYFHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSIGRFGYGENTINVTNWFPIACVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLIDDKHDNKKMLYEIEAENVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFKPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39603..40400
                     /locus_tag="PC545_000466"
     CDS             39603..40400
                     /locus_tag="PC545_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2455258.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     VDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTESDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSSTGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40413..42515
                     /locus_tag="PC545_000467"
     CDS             40413..42515
                     /locus_tag="PC545_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HDF2455259.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     AYQEYLSGLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELNSYIEIFKTKWLFTSIIVSMTSSVLFVKLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVVMISLIGSSNFQDIFYNLFSNVFNKMGTNLMSAL
                     LFIFLMHFMWFFGIHGSNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGSLLCLVIAIFLSEKRINVRKLAKIASIPALFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTVTSYVAMSTGIVPCTVSAVEWTSPIFLSGYMSTGSIRGSLLQVFNLCVG
                     VMIYIPFIKMSQNRYTYMFKNNIENLTNLLKRYELTGEQPNLLNHNGKIGSISKMLAS
                     DLKFAMKRGEIELFYQPQVNYNGNVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACIDLKKSEKLLRNPVKFSVNISPDQLDDPKLPEQIKNIISSNDINPNM
                     LGIEITEQIALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENIDQRDKLYGLGCEIYQGYYYS
                     KAIPFEEFVEYANQEREI"
     gene            42583..43062
                     /gene="rlmH"
                     /locus_tag="PC545_000468"
     CDS             42583..43062
                     /gene="rlmH"
                     /locus_tag="PC545_000468"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDF2455260.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSVKEMEIIKDKEGKKILGKIKHNSYVIALAIDGKGLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSKMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43211..43522
                     /locus_tag="PC545_000469"
     CDS             43211..43522
                     /locus_tag="PC545_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455261.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMESAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43570..43887
                     /locus_tag="PC545_000470"
     CDS             43570..43887
                     /locus_tag="PC545_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2455262.1"
                     /translation="MDEKTIEISFNIIGYAGEGKGLAFEAIKEAKNGNIEKAKELLKE
                     SREVINKAHRYQTELIQNEASGNRTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
     gene            44100..44801
                     /locus_tag="PC545_000471"
     CDS             44100..44801
                     /locus_tag="PC545_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455263.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKKTIS
                     IEKIINDTNSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLAKDILLGRKRKTYDENDNIVELSFA
                     RYNTEVQNYVIKYEI"
     gene            45025..46326
                     /locus_tag="PC545_000472"
     CDS             45025..46326
                     /locus_tag="PC545_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2455264.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKAILDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVEPVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISVALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSAHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGVIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            46463..47557
                     /locus_tag="PC545_000473"
     CDS             46463..47557
                     /locus_tag="PC545_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDF2455265.1"
                     /translation="MERRLGISVYPEHSNMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILQISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATATKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVNKYQVVFNVVTEEKTSEVEKTIMANEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTTIFKRGDVVIGNDDFGKYKGELQLILQDCADERK
                     NKVGRIKEDEIMLLDFIEPWSKFKLILEGE"
     gene            47559..48692
                     /locus_tag="PC545_000474"
     CDS             47559..48692
                     /locus_tag="PC545_000474"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HDF2455266.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSSMMNIDEYELKRAVKKYKDFVVGIKARMSK
                     SVVISNDVEPLKVAKRIKNELNLPMMVHFGSSPPTIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDIAMKSKDAGIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIIDKVTKNAADAISLKNKGLIKEGYDADLTIFDV
                     VNEEKELQDSLNKSVITSTSIKPRAVVVNGEYLNIGKSIGENE"
     gene            48710..49819
                     /locus_tag="PC545_000475"
     CDS             48710..49819
                     /locus_tag="PC545_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HDF2455267.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELINKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTIKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRSGEIAIYT
                     RDYKANEGKIEIDIRSVTGSDLDTIYNRIKNIVES"
     gene            49884..50624
                     /locus_tag="PC545_000476"
     CDS             49884..50624
                     /locus_tag="PC545_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HDF2455268.1"
                     /translation="MNCIPQYYKNRVCLNVLAGSIKNAKEVYDACDGYVLIGVLSKNY
                     STVEAAIEDMKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQNDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVPIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGETKVEDIGVLYKIMKELLG"
     gene            50629..51621
                     /locus_tag="PC545_000477"
     CDS             50629..51621
                     /locus_tag="PC545_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDF2455269.1"
                     /translation="MNILAFGEIMMRLSVPDYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANIRKLGVKDDFITYGGNHIGVYFLENGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEEMDIVLICGIAMQLTDSIKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERTYDDLVNDYKKILPYCHGLFASYRDINKILNIDSLNIKSSSE
                     LIKKEYDIGIVAGTMRRFDDMNNRFIKGYISNDDGYFESREYKLEIYDRVGAGDGYVA
                     GIIYSYLENMKSEDIVEFGVASSVLAHTTYGDNSLVSTGHVLRLMNNEKIDILR"
     gene            complement(51809..52690)
                     /locus_tag="PC545_000478"
     CDS             complement(51809..52690)
                     /locus_tag="PC545_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2455270.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKALEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEYK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVSSNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            52963..54351
                     /locus_tag="PC545_000479"
     CDS             52963..54351
                     /locus_tag="PC545_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455271.1"
                     /translation="MEDKFYAKGNGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLIYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLVVLDVEDLTRPRCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKPWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFPGPRLAVTEDLIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            54379..55332
                     /locus_tag="PC545_000480"
     CDS             54379..55332
                     /locus_tag="PC545_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2455272.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSMCVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNIIGTKVAEAIGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTRLVQVHQIGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYLEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTQEFKERKTSYNFIETLTKEKQAGVSLG"
     gene            55392..55616
                     /locus_tag="PC545_000481"
     CDS             55392..55616
                     /locus_tag="PC545_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455273.1"
                     /translation="MNEKPSFKQIEVILAVIFLVLTYYKVRLTPAFIVIFIPLYLSYF
                     AVSMIVNQIAIKLLEIRQAREATNYNIGNG"
     gene            55609..56121
                     /locus_tag="PC545_000482"
     CDS             55609..56121
                     /locus_tag="PC545_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDF2455274.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQEDGLKIMFGFWCGDLWAVLAILIMGYMNFNQNLELFLTLSILGFFAVVIAS
                     LFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAVAMAVGVWYVGAGVDLFTIY
                     ILSKDRKKGN"
     gene            56153..57007
                     /locus_tag="PC545_000483"
     CDS             56153..57007
                     /locus_tag="PC545_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HDF2455275.1"
                     /translation="MAMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDVENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGISKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVS
                     ELWFEGEEEWYKAVVEETKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            57541..58335
                     /locus_tag="PC545_000484"
     CDS             57541..58335
                     /locus_tag="PC545_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2455276.1"
                     /translation="MYTIGQVSKFLGISRDTLKFYEDKGLVNPKKNDENGYRIYNQVD
                     IYDIATINFYREIDIEIKKIQEIRKSKSINNLELLLEEKEQIILKEIEYKKLLLKKVQ
                     LVKEDCDKIKTYFGKYTIKEMKPLEVKGKIKHVTNYEEYEALKENTNNLKKAVTLTDL
                     RRVINFNEEGIIEEKFIIVREVDDYNKIIEGEVLSHPNCIYTIIEDGRWSTGGENIDN
                     KVEDRLREIAIENECELLGIVYVNILLTTYEEGIERIFLEIYAPIK"
     gene            58439..59245
                     /locus_tag="PC545_000485"
     CDS             58439..59245
                     /locus_tag="PC545_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2455277.1"
                     /translation="MRTEKSLKRPIASFIILTNIIFLPLFLLVVVTIMLKLPTVISDI
                     ALCISAWSSTFAFMILFKKIYPGKKFLVHVKDRFRNKLKFSTVSIIISIQVLIAVVVI
                     FILASKNHGIMPNFTVSSWGAFIYLFLKNLFAGSLGEELGWRGFVQNHLQKRHSPLKA
                     SITVGFWWGMWHLPIWFTTGFKGLDLIKYIIFFMIAIISISIIMTTFYNLNKTLIIPI
                     AIHQLFNFLIGIINEDLIELINYYAMLYLIFAIALIVINPGNVLYKNIRE"
     gene            59307..59828
                     /locus_tag="PC545_000486"
     CDS             59307..59828
                     /locus_tag="PC545_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2455278.1"
                     /translation="MVLLIVDAQKLITNERLYKFNEFVVNVENLIDTARKNNIEVIYV
                     RHDDGVENELTKGKNGFEIYEKFKPCNGEKIFDKKVNSAFKETGLLEYLTNNGEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPAYSNTTVDNFFMSAEQSYKYYNEFIWNERYA
                     KCISLEETLRKMK"
     gene            60243..62330
                     /locus_tag="PC545_000487"
     CDS             60243..62330
                     /locus_tag="PC545_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2455279.1"
                     /translation="MVVDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKIN
                     DMLKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKTERLLYMYLMLF
                     INMEDISINHFINSIKVSRSTVNLDFKDLIPELEKKNIKVKNNRINGYYIVGNEMEIR
                     RVLIKNIIETLSNEKSSRVFDIFIKEYNLDSFEEAKKIILKLIKKYEITFVEDRLIEF
                     IYIFIFLKARMYSTRIVTQENVDIPNISVMSSMKEYKFAKELLEVYGSEDKIKPHNIM
                     YISAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFR
                     PAYYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHF
                     ATLFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENIS
                     EPVDIVFTTNYNAEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEV
                     MDIIKKYANVTSESILSSELLAYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNW
                     EEAIRQSASVLVENNKVTEGYVDAMINSAKASGPYIVITKHVALPHARPEAGSKEIAI
                     GIATLDSPIEFGNNENDPVKYVFCLSAIDNNSHLRAMSELVELLEEDEFFKVLDSAKE
                     AKEIIDFIKTHEL"
     gene            62345..62632
                     /locus_tag="PC545_000488"
     CDS             62345..62632
                     /locus_tag="PC545_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455280.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIIKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKNLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            62659..63930
                     /locus_tag="PC545_000489"
     CDS             62659..63930
                     /locus_tag="PC545_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HDF2455281.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFISAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNTSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            63988..64587
                     /locus_tag="PC545_000490"
     CDS             63988..64587
                     /locus_tag="PC545_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2455282.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACELILEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTSAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKATLKTLKVNGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIILQSLSVVLQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            64592..65497
                     /gene="dapA"
                     /locus_tag="PC545_000491"
     CDS             64592..65497
                     /gene="dapA"
                     /locus_tag="PC545_000491"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2455283.1"
                     /translation="MKYKGIICAMITPFDENQNINSQATCQLIDYLIEKGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVVDLSKRMEAIGASALS
                     IITPYFVTPTQQELILHYKTIANSVNLPIIMYNMPSKTGINIEPESVRELSKIKNIVG
                     IKDSSGKLDNMKAYIEATKNENFSVFSGSDSLILDSLKAGSQGAVAATTNFLTEIGIS
                     IYENFMKGDLEEAQKAQSSIEELRRILKLGTIPSVMKKTIVLNGINVGTARLPVTEPT
                     GEVLEEIKRVVENYRTILNKSSENR"
     gene            65603..66235
                     /locus_tag="PC545_000492"
     CDS             65603..66235
                     /locus_tag="PC545_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HDF2455284.1"
                     /translation="MKKMNITKRMSECGALAIVREENLNRACEIAEGCIKGGITVIEM
                     SYTLNNAGEIIQGLNKKYGETLCVGAGTVLDSETARHAILHGAQFIIAPNYNEGVAKI
                     CNRYQIPYAPGCTSLTEAVDALSLGATFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINIENLHIWLENGVDVCGFGGLLTKGSVEDIAKNAKKIREIIKNIRKI"
     gene            66713..69220
                     /locus_tag="PC545_000493"
     CDS             66713..69220
                     /locus_tag="PC545_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2455285.1"
                     /translation="MKEDIRLHRMCKKEQATKNEVISEDILKSMSIGVSKHFLDDSFT
                     IIWGNTSFYQLLGYTEEEFLVKFSSLKDYYEQNLYHFESMKNHFLEAYHHGEKSIEYQ
                     ICISANNGDPVWVIMTGILIESQDNEKSMVYILYSKINELKIKQEELSDMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKHSCNTLQASANELIGRKCYEVIQGRTSPCPFC
                     TNSYLKKDDIYEWEFYNKKLKRTFIIKDRMLNWQGRRVRIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRLDARDYLTILWCNDIFLNMIEYTKEQFAEELNNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEAKIYTRSREERIWTVTLCYISGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQHISEKDALTGIYNRAETERQIKKYFEKNLNVMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMRDSDVVGRIGGDEFTIFMKNISSVKDAEKKAEEL
                     LYMFRHLFQKEKSFLKVTCSIGIAIYPKDGTTFKEIYARADKALYQAKNMGKNNYVIY
                     NQDYFKELEEFDCSSLGTVIDSEKQYAEYPDNLTRYIFRMLYQTDDIDQAINMILEVV
                     GKQFDVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNSNYQDYEDYEKLFEDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDRVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNNKLNQQIQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKFQIGQPYYLDSIDIHKISIIWNKKEAYLCTSTI"
     gene            69562..71724
                     /locus_tag="PC545_000494"
     CDS             69562..71724
                     /locus_tag="PC545_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDF2455286.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNETTSISVMF
                     KDYKKLNKNYSKWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGKFPN
                     TNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFGKISEVGESGILYISTQNEDTIN
                     KILRELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSREA
                     SILRLNGYSKLDIIARVIKNILRIMILSSVTALLIYIIYILKLNIGVRACLYFAMFSI
                     ICILINILISILIVGFNSRSSKYVLSLNGKKSYGFVNIMHFTLKIVFVSFLILSINNC
                     ILNYNMLQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKNLVEEK
                     DAFLIDSTNFEKLEDGSYMYEINSKGKNPEVSQYGKNIKINKNYLKVNPIYSNGKEIF
                     NLIDYDDKTINLLVPKKLQVHENEIKKEFRELFYFEKIEVENMYNKELNRALNKTKKS
                     DLNVNIIYVDNNQSYFTYNSLVMDDNRNLIDDPIAIVDIDNIDDSFYLSYISRCVYFN
                     SKKLDALADISSIIEAQGVEAHIQSLHAVYNEYGLEINKLEKYLNSEIFTIIIIAISN
                     LMITYNIVASYYERNKYKLYIKKIFGYSTTARSMLLIVSLILTNIIPIGIISTRVDLS
                     NNIILFGLLILVIEVIVSIVLDKIISNSSFNKIIKGEH"
     gene            71729..72346
                     /locus_tag="PC545_000495"
     CDS             71729..72346
                     /locus_tag="PC545_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455287.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVENNLKIAL
                     KYQKNINHNKKINYALESVGLRKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNKNGKTIVFVTHDLELSEQADRVIKL"
     gene            72535..72879
                     /locus_tag="PC545_000496"
     CDS             72535..72879
                     /locus_tag="PC545_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDF2455288.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLGAEEDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSSIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVVKKA"
     gene            73449..74315
                     /locus_tag="PC545_000497"
     CDS             73449..74315
                     /locus_tag="PC545_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2455289.1"
                     /translation="MIDIHKNIYDNKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCINLQPDFKCSVHNSLMKRGFKGCTAYDCFGSGQKVAQVTYKGIDWMQSSELT
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKIDLIIEETEKITNMSPEQIINLD
                     IISHRIKVNLLLSQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKINLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PASLIRPKYWEK"
     gene            74428..74832
                     /locus_tag="PC545_000498"
     CDS             74428..74832
                     /locus_tag="PC545_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HDF2455290.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNKCYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEAIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            74999..75448
                     /locus_tag="PC545_000499"
     CDS             74999..75448
                     /locus_tag="PC545_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455291.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIESLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKVFDRSEIDGQGNPFPILHMKLI"
     gene            complement(75604..75981)
                     /locus_tag="PC545_000500"
     CDS             complement(75604..75981)
                     /locus_tag="PC545_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2455292.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLDSNPNNPIMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSREAIEEISVKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            76243..77160
                     /locus_tag="PC545_000501"
     CDS             76243..77160
                     /locus_tag="PC545_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HDF2455293.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYRYELMKPFEFKWKC
                     MNVPIVARQKGGYDVIIASEMLGVLSPKDIDEKQKKNINVLSADKIWATCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGLAENFATWMFGEEMLGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVKEFWE"
BASE COUNT        29614 a         9141 c        12869 g        25713 t
ORIGIN      
        1 atataaatta acctactgtt taattttcaa tgttcgtgtt tatttcttgt tccttttttc
       61 aaggacaagt aatactatac catctttaaa acagtaggtc aatacttttt ttatattttt
      121 ttataaattt ttttcatatt cttttttcag cttctctact acctttttct ctagtctaga
      181 aacagtcatc tgtgatatcc ctagttcttt agctatacta gattgtgttt tatcaaagaa
      241 aaatctatct ttaaaaattt taacttctaa ttcatttaaa gtttcaataa acttattaat
      301 aaaatcttca tactctatac ttccaaagtt accttcttct tttccaatct tatcaataag
      361 agttatatct ttatcctctg aatcatcatt acttgatgaa tccaaagaca ttggttgata
      421 tccataagaa gcctccatag cctctaaaac atcttcttca ctaacgccta tataatcagc
      481 tatatctttt acctttggat gcttcttatt ttcttgttct aatttaatct tcgcatcact
      541 tatctttttg cttaattcct gtattcttct tgggactcta agtgtccata ctttatctct
      601 aaaatacttt tttatttctc ctactatagt tggtgttgca aagctggaga actcaaatcc
      661 tttctcaaca tcatatctat caatagcata aattaatgcc aatgaagcaa cttgatatat
      721 atcttcaaaa tcaacacctt tgtttatata cttcttagct aacaatcttg ctaaatacag
      781 atgtctttca ataagaatat ttctaatgtc tacattttta tttttattat ataaattaaa
      841 tagctctttt gtatccatat ttaggtaatg tgtagcatta gctacatttt tcattaaata
      901 tcaactccta aatatttagt catttttatt tttgtccctt gattatgttc tgattcaata
      961 tctacatcat ccattaaagt tttaattata aacaatccaa gaccactctc ttttggattc
     1021 tctaaatctg gtgtacttat tgagctaaca tcatatcctt ttccattatc ttgaatttct
     1081 atattaattg cattatctaa aatattaaat actatattaa atacatcatc tttactgtgt
     1141 tttattgcat ttgtacatgc ttcagatact gctaccttta tatcttctat atcatctatt
     1201 ggaaaaccca tcttattggc aattccagat gctgttaatc ttattatact aacataatct
     1261 ggatttgtag taatttccat ctttatagtc tcacaagcca aagtttatcc ctccacttta
     1321 aatattttat ctagacctgt tatagtaaaa atctttctta cgttactctt aggatttact
     1381 atgtatatct ctttttcatt tatttttaat ttttttaata cacctatcat agctcctaag
     1441 cctgttgagt ctatataatc taaatcttta agattaatct ttacatctaa catatttttt
     1501 tctattaaag catgtaaatg ttcctttagt ttatctgctg tagaaacatc aagttctcca
     1561 tcaagacaaa cattccaaaa tttattttgt gaatctaaat ttgaatcaat attcatagac
     1621 atgtaaaaac ctcctatttc cttttaataa ttttaaatat atttcctata ctcaatattt
     1681 tattaattac ataaactata tcatctattc cctttgatat tgatgatgca ttttcaacaa
     1741 tatcttgtat atgtctttca ttataatcta caatcttata tgccttttca acaaccttat
     1801 ttatgctgtt tagagtttta gctaaataaa ttgctactat aagagcagag cttcctacta
     1861 acactgctcc aatttgccac ccccatgcat tcattagtta acatctccct catcatcaaa
     1921 gcttttactt attactatat cttcttctga aatctttata tcttcttcat tattatcatc
     1981 atccactaaa tttattattc tctctttaac atcattaaat gtttcatgta gaacttcttt
     2041 tggattttct ttaacatcat taaacttctc ttttgtttct tttcttagtt cagaaccttt
     2101 tttaggtgca aaaaacatac caaatataaa ccctaaaata gctcctaata ataaaaatcc
     2161 acctttttta caacttcttt tattgcacat aacaattact cctttcaatt ttaaattttt
     2221 atctaactac attatacaaa aagaatagga tatttatcct attcttcttc aatatttatt
     2281 ttggcaatcg aaactacatt gacttcatca ttcgtcttca ttaatttaac accacttgta
     2341 actcttccaa acagtgatat ttcattgact cttattctaa taagaactcc atcagaattt
     2401 ataatcatta tctcatcatc ttcgttgacc atatctgcac ctacaattgt accagttttt
     2461 tcagatatat tataagtttt aattccttta ccagctctta tttgacttct atactcttct
     2521 atatttgttc tctttccaaa accattctta cttacaacta acacatctgt acctttactg
     2581 caaaggttca tagacacaac aaaatcttct ttacttaaag ttataccttt tacacccatt
     2641 gctgttctac ccatatatct tatatcattt tcatcaaatc ttatagacat accttcttta
     2701 gttactaaaa gtacttcttg ttttccatct gtaagttcta ctccaataag ctcatcatca
     2761 tctcttaaac ctatagcaat aagaccagat ttatttatat ttttgaactc ttctctctta
     2821 gtctttttaa caataccttt tttcgttgca agtaataaat attcattttc atcattacca
     2881 tcaataggta ttagagtagc aattttctca tctgctgaca attgaagtaa attcactata
     2941 gcagtacctt tagcctgtct cttaccttct ggtatttcat atgcatttaa tttaaatact
     3001 ctacctttat ttgtaaagaa taatagtcta ctatgagtag ttgtagttac caagtgcctt
     3061 acaaaatctt cttctctagt tgtaagtgct gaaatacctc ttccacccct tttttgactc
     3121 ttataagtat cagatggaag cctttttata tatccaaagt gagtaagagt tattgctatt
     3181 tcttcatcac ttataagatc tctcatatct atttcgcctt cagcatgtct tatttctgtt
     3241 cttctctcat cagagtaatt ttcttttatt attgtaattt catcctttat tacatttaaa
     3301 agtaatcttt catcagctaa aatctctttt aatctattta tcttcttgat taaatcttca
     3361 tattcagctt ctatcttatc tctttctaaa cctgtaagtc tttgaagtct catatctaat
     3421 atagcttgtg cttggatttc agttaatttg aatttttcta ttaaacctag cttagcttct
     3481 tgcccagtct ttgaagctct tatcaagctt ataacagcat ctatattatc taaagcaatc
     3541 tttaatcctt ctaaaatatg tgctcttgct tcagctttat ttagttcaaa tttagttctt
     3601 cttgtaacaa catcttcttg atgtttaata taatgatata atatttgttt aagatttaaa
     3661 actcttggct gaccatctac aagtgcaagc attattatac taaaagtatc ttccatttga
     3721 gaatgtttat acaaattatt taatactata ttagcattag catccctctt taattctata
     3781 acaattctca taccatttct attactttcg tctcttaggt ctgatatacc ttctattctc
     3841 ttttctttaa ctaactctgc tattctttca accagtttag ccttatttac ttgataaggt
     3901 atttctgtaa ctattatttg ctgttttcct tttggtagct cttctataaa agctctagac
     3961 ctaactttaa cttttcctct tccagttctg tatgcttctg ctatactttc ttttcccatt
     4021 ataattgcag ctgtagggaa atctggtcct tgaacaaatt ttattaaatc atctacacta
     4081 cactctggat tatctatcaa atatacagtt gcatcaatta cttctgctaa attatgtgga
     4141 ggtattgaag ttgccatacc aacagctata ccatttgagc catttactaa taaattagga
     4201 tatctagctg gcaatactga aggctctttt aacgactcat caaagtttgg tttaaagtct
     4261 acagtttcct tttcaatatc tctcagtagt tctaatgata atttactcat tttagcttct
     4321 gtataacgca tagcagctgg tgaatcccca tcaacagaac caaagttacc atgaccatct
     4381 actaaaagtg ctctagttga aaaatcttgt gccattctta ccatagcata ataaacagca
     4441 gtatctccat gaggatggta cttacctaaa acgtccccaa caatacgagc tgacttcctg
     4501 tatggtttat ctggagttaa atttaactca ctcattgaat ataatattct tctatgaact
     4561 ggctttaaac catctctaac atcaggaaga gcacgtccag ctataacact cattgaataa
     4621 tcaatatacg attttttcat ttcttccgct atttcaatag ggagtatttt gttattttct
     4681 tccatgtata ctaatcccct ctctctatat atctaagttt ctaacaaatc ttgcattttc
     4741 ttctataaat tcttttcttg gagcaacctt atcacccata agcattgaaa atacttcatc
     4801 tgcaattgca gcatcttcta cagtaacttg taataaagtt cttgtctcag gattcatagt
     4861 tgtttcccat aattgctctg cattcatctc tccaagacct ttgtaccttt gaagttcaac
     4921 accatttctt ccaatttcat ctaataagat atttaactct ttatctgagt aaacataatg
     4981 ttccttcttt tgctttgtca ctttatataa aggtggctgt gctgcataaa catacccctc
     5041 atctataaga ggtctcatat atctaaagaa gaatgttaat aataaagttc ttatatgggc
     5101 tccgtctaca tcagcatcgg tcataattat aattttatga tacctagcct tatctatatc
     5161 aaaatcttct ccaataccac aaccataagc tgttatcata ttttttattt catctgaaga
     5221 tagtatccta tctagtcttg atttctcaac attaagtatt ttccctctta atggaagtat
     5281 agcttgacta tttctatctc taccttgttt agctgaacct cccgctgaat ccccttcgac
     5341 taagaatatt tcacttttag atggatcttt ttctgcacaa tctgcaagtt ttccaggtaa
     5401 agatgtactt tctaatacac tttttctcct tgttaattct cttgcttttt ttgcagcttc
     5461 tctagctctt tgtgctctta aagctttatc aactatgatt cttgctgttg atgggttttc
     5521 ttctaggaaa gaccccagtt catcaactgt tacactatca actatacctc tcataaagct
     5581 attacctagc tttgttttag tttgaccttc aaattgaggt tctggtaatt taactgatac
     5641 tactgctgta agaccttctc ttatgtcttc accaagtagg ttaacatcat tttcctttaa
     5701 aaatttattt ctcttagcat aatcatttac agttttagta agagctgctt taaatccact
     5761 taaatgagaa ccaccttcat gtgtatttat attgtttgca aaagaatata tgttctctgt
     5821 ataaccatct gtatattgca tagctagttc aacaaaacaa tcatcaacct ttttatctat
     5881 ataaactatg tcatcatgta taccagttct agatttattt aaatacttaa tatattctac
     5941 caaaccacct gtatagtgaa attcttttct tttttcttgt ccttctcttt tatcttcaaa
     6001 tactattttt attcctttat tcaagaaaga aagttctcta agcctatact ccaatgtttc
     6061 atatttaaac tctatttcat caaatattgt ctcatctggc ataaattgaa tcattgttcc
     6121 tttatcttga gattctccaa ctacttcaag cttagatgtt ggcaatcctt tctcatatgt
     6181 ctgcttatat ctctttccgt ttaaataaac ctctgctacc atccattttg acaatgcatt
     6241 aactacagaa acaccaactc catgaagacc tccagatact ttatatcctc cgcctccaaa
     6301 ctttcctcct gcatgaagat tagttaatac tgtttctaaa gttgatttac cagttttagg
     6361 atgtatttca acaggtatac ctcttccgtt atcttttact aaaacactgc catcttcatt
     6421 aatagataca tatatgtctg aacaatatcc ttgtaaagcc tcatctatac tattatctac
     6481 aacttcataa actaagtgat gtaaacctct agggcttgta ctaccaatat acatacctgg
     6541 tctttttcta acggcttcta atccctctaa tacttgtatc tgacttgcac cgtattcttg
     6601 tttcatttaa tttcctccat tctctatgct aataacatcg ccattttcaa tattaaatat
     6661 tgtaacattt ttttcatcaa atattttttt atgtgaaact tcagcacttg ttataaacat
     6721 ttgaacattg cttaaattat ttactaaaag tttttgtctt gcttcatcta actcactaaa
     6781 aacatcatct aaaattaata ctggatattc ttcaacttca ttttttatta attctatctc
     6841 tgataacttt aatgatattg aagcagttct ttgttgacct tgtgaaccaa aaagtcttgc
     6901 atctaaatca tttatgaata tatttaagtc atctttgtgt ataccatatc tggtagtctt
     6961 agattctata tcattaggtc tattcgatga caacttggct aaaaatttat tatatactgt
     7021 atctatagta tcttcatcag ttatatttat ttgattttta tatcgaattg ataatctttc
     7081 aactccatta gtcaaattca tatgcatatt ttcagatata ttagctatct tttttataaa
     7141 gtctctacgc aaaatataaa tataacttcc atactttgct aacgtatcat catatacatc
     7201 taataaagct tcatcaacat gtatattttt aagcacccta ctcctttgag aaagtgtttt
     7261 attataattg gtaagatatt tataatattt aggtataatc tgactaatct ctttgtctat
     7321 aaatgccctt ctttctttag gaccctcttt aaccaacctt aaatcctcag gagaaaatat
     7381 aacaacattt aagttgccaa gtagttcttg tatcttttgt aaaggaactt tatttattct
     7441 tatccctttt ttatcttttc caataattag ctctattaga ctatatttat tatattttga
     7501 aaaacttcct ccaatatata agttttcact attaaatcta accatttctt tatctttgtt
     7561 agtcctaaat gattttccaa aagaaagcat ataaatagat tctactatat ttgtctttcc
     7621 ttgaccattt ttaccaacaa gtagatttac tttcccatta aattccagat gcaacttttt
     7681 ataatttcta aaattaacaa gttgcaaact tttaagtttc acaaaaagtt ctcctttttt
     7741 acaatacttt tattttagtt ccttctattt caactatatc tcctgatttt atcttttttc
     7801 ctcttcttaa ttctatttca tcatttactg tcactaaacc ttcttgaatt aaaaattttg
     7861 catgacctcc tgttgatgca atctctgcta atttaagaaa ttgatccaac tttatatatt
     7921 ctgattctat agttatttca gtcataatag ttcctccttg tcactatttt aaacaaattc
     7981 ctattgatac actaaaagta tattatacca taaatatgct tataaaagga gttgtctaag
     8041 attagaaaaa ttttctacaa aagacaactc cttcatgtct gctatatatt tgatgatatt
     8101 cttactggaa gcagtaagta aatataatta acatcatctg ttggtttaat tatacaagga
     8161 tttacatttg tagtaaattc tataaatatt tcctcattat ctatattttt caaaccttcg
     8221 ataaaatatc tagaattgaa agctatatct aggtaatctc catctaaatc tatttcaact
     8281 tcttcataca catttccttt ttctgtatta gaagtaattg ccattacttt atctcttata
     8341 gataacttta taagattatt tttttctgat tgagacaata aagacgctct ctctatacta
     8401 tttaaaagtt ccttagtatt taatttaact ctgctgttat gttctcttgg caataatttt
     8461 ttataatcaa taaaatctcc ttcaagcaat cttgtaataa ttttcgtatc attaattata
     8521 aaaatagcat ttttttcgtt aaatccaact ttaacattat cctctccaga aagcaaacta
     8581 tttacatcta taagtgtctt tccaggaatt atgactttta tattttcagt taaactatca
     8641 accgaacaac tttttacagc taatctatat ccatcaattg ctactaaatt taagtttcta
     8701 tctacaattt ctaaaagttc tcccattaaa acaggttttg tttggtcctg tgagatagca
     8761 aatacagttt gtttaatcat attttttagg atttcttgag gtatgctata taaatcttct
     8821 tcatttaatt ctggtaattt aggaaattct tttgctgcat aacctttaat tttaaatctt
     8881 gaatttacac aatttatgta aatattattt tctgaatcag tttctatctc aacaaaggaa
     8941 tctggtaatt ttctaattat atctccaaat agccttgcat ctacaacaac atcacctttt
     9001 tctatgattt ctgcttgtac ataagtttct attcctattt cagcatcata tccagtaagt
     9061 tttaattgac cttcttctgt agatataagt atacctttta ataattctat tgttgttttt
     9121 ccattaatag ctttttgagc aattccaatt ctatttgcta ggattttttg attacaaatt
     9181 attttcaatg tggataacct cctctggctt tttatatata aaaataagtt aaaatcatag
     9241 tagtaatagt agtaggtgtt gttgataatg ttaataagta tgtataacat tataaataca
     9301 aagatattaa catgtgtata agatgttaat atctttgtta ttttagactt gataatttgt
     9361 taataatttt tgttcaaaaa aagtattaac aggttattca agagatataa acatacaatt
     9421 gtgtataaat tatcccttca aatctgatat aattttgtct attttagtct taatttcttc
     9481 actttcttct atatccttag atactttgtc atgagcatga attactgttg tatggtctct
     9541 acccccaaac tcttcaccta ttttaggaag ggataaatca gtaagctcgc gcgttaaata
     9601 catagctatc tgtcttggat aagctattga acgtgttctt tttttagaat taaaatcttc
     9661 catctttaaa ttgaatacag aagatacttt ttcttttatt ctgagaacat ttatttcttc
     9721 atttttagaa gttgttataa tatcttttaa agcttctgta gcaagatcaa aagatattac
     9781 cctattgcta agagaagaat aagcaaccac tctagttaat gcaccttcta gttcccttat
     9841 gtttgactta atattttttg ctatatatga cataacctca tttggcacat ctatacgttc
     9901 tagttgagcc ttttttctaa gtatagcaat tcttgtctca aagtcaggtg cttgaatatc
     9961 agttataagt cccatttcaa atctcgatct tagcctatct tctagtgtag gtatgtcttt
    10021 tggcggtcta tcactagata taattatttg tttgtttgct tcatgaagag tattgaatgt
    10081 atggaaaaac tcttcttgag ttctttcttt tcctgctata aattgaatat cgtcgattag
    10141 taatacatct acgtttctat atttattcct aaattcttcg tttttatcgt cttttataga
    10201 gtttataagt tcattagtaa atttttctga agaaacgtaa actacttttg agtctttttt
    10261 ttgactaaca atatgatgac ctatagcatg cattaggtga gtcttaccta atccaactcc
    10321 tccatacaaa aaaagtgggt tataagcttt agctggagat tctgcaactg caacgcatgc
    10381 agcatgtgca aatctattac tattaccaat aacgaatgta tcaaatgtgt attttggatt
    10441 tagattgggg taaagaactc tataatttaa atctgtgcta tcagaattta aatcagcaac
    10501 ctctttttcg ctcaatacaa atttaatatt atattttttt aatgacagtt gactaatagc
    10561 atcttcaatt aaatgtaaat atctattctc taagatatct ttattaaaat cactaggagc
    10621 cagtaagatt aaatcattaa gatgtatttt taatggtacg atgtttttaa aaaaggtatt
    10681 aaaacttact gaagttaagt caccttttat taattgtagg gttttgtccc ataaagaaac
    10741 tatatccata atataaacct ccgaacagaa taattaatat tttccacaaa gttattaaca
    10801 gaaaagcaaa aatatatata aattttacag aataactaaa tttatggata atggtgttga
    10861 taaattaata atagtataaa ttattaaaaa aatatgaaaa gatataaaca atatgtagat
    10921 aaagttataa ataacataaa catacaataa atggtgatta taactagagt attgtcagta
    10981 atcttaaaag ttatacacaa aagtatccac aagctgtgaa taatttctaa taagttgata
    11041 acatttatgc acattatcaa caagtatatt tataatatca aaatatatgt caaacttcaa
    11101 tgatttgtaa ctaatttatc cacaatatta actaattgtt gataaaaata taaacatgtt
    11161 aaaattactt tatttgtgta tataattgaa aaaaataatt tagaataaaa ttaaatagat
    11221 ttttactagt attttcaata aaaaaaatac ataatatata tatggtagat tttgtgatat
    11281 aagcgtccca aaaactcgtt gacaacagta gtaaaagtat ttataattat atgtgttatg
    11341 tttactttga gggtaaagtt attattttta taaacggaaa ataaaatctg aaaggcggtg
    11401 caaaataatg agtaaaagaa cttatcaacc aaaaaagaga caaagaagta aagaacatgg
    11461 tttcagaaaa agaatgaaga cgtctaacgg aagaaacgtg ttaaagagaa gaagagctaa
    11521 aggaagaaat agattaactc attaattaca aaagggccgc tgcaaaggtg gcctttttgt
    11581 gtaaataatt acaattcata gctgtgttta tggaggttaa ctatggactt taataggact
    11641 aaagggttga aaaaagactc tgattttaga aaagtatata aacacggcaa atcttttgca
    11701 aacaaatatt tagtaatata tatacttaaa aataaatcag attatagtag agtaggtatt
    11761 tctgtttcaa aaaaagtagg aaaagctatt actaggaata gagtaagaag attaataaaa
    11821 gaagcctaca gattaaatat tgatgaaaaa attaagcctg gatatgatat agtatttata
    11881 gcaagggtat ctagtaagga tgctactttt aaagacatag acaagtctat aaaaaattta
    11941 gttaaaagaa cggatatttc tatttaattg tatgctataa atatggaggt taatgacttt
    12001 gaataaaata atttatatat taaaggaaat aagtaagcat ttatcgaatt tatgtattta
    12061 tttagtgaga ttttatcaaa agtatatatc tcccttaaag ggacctacct gtagatttta
    12121 tccaacttgc tcacaatatt caatagaggc gtttaagaaa tatggatttg taaaaggtat
    12181 gtatttaacg ataagaagac ttttaaaatg tcatcctttt catccaggag gttacgaccc
    12241 tctaaaataa aagagagtaa ctattaggag gtataaaaaa attgaatata ataggaaatt
    12301 tcttaggatt gattcttaag gtaatatttg aatttgttaa tcattatggt atttctataa
    12361 tactttttac gatacttgta aagattattt tattaccatt aacaattaag cagactaagt
    12421 caacaaaggc aatgcaagat atacaacctc gtataaaaga gatacaagaa aaatataaaa
    12481 ataagccaga aaaacaaaat gaagaaattg ttaagttata tggagaagct aaaattaatc
    12541 cactatctgg ttgtttacct ttattaatac agtttcctat attaatagga ttatttagcg
    12601 ttttgagaga accagttgct catggcgttt ttgcaaacaa agctgcattt ttagcagctg
    12661 acaatgggtt tttatggata aaaagcttaa catcaccaga ttatgtatta gcagtatttt
    12721 caggagcaag tgcatatgtc atgcaaaaag taatgacacc aaaagatcag ttacaaggct
    12781 ctatgaaagt aatgacatat gtaatggcag gtatgtcttt ctattgggga tttatattcc
    12841 cagcaggact tacattgtac tggacagtaa gtaacttatt ctcaatagct caatattact
    12901 taataatgaa tcctttaaaa gctaaactgg ctgctaactc taaggaggaa aaagttaatg
    12961 agaaaaagcc taaaattaat aaaaagtaag agtaaagagg aagctataag taaagctata
    13021 gcagagttga atttaaaagc agaggatata gaagtagaaa tacttgaaaa tccaagtaaa
    13081 gggtttttag gttttatagg tgctaaagat ggtacttatg aaatttttgt tatcgagagg
    13141 gaagtagatg tagctaaaaa ttttatagag gtaatgttga aaaatgcaaa tgttgatgcc
    13201 aaagtcaatg tatctcaaaa agataattta atcatggttg atattgaagg caaagaagct
    13261 gcaagtttaa ttggtagaag aggggaaact ttagattcta ttcaattttt gactggatta
    13321 gctcttaata aaataaataa agattctcat acaagagtac ttgtagatat agaaaattat
    13381 agatcaaaac gagaagaatc tttaataaga tatgctaata aagttgctag agaagtagcg
    13441 aaaacaagaa aaactaaaaa attagattat atgaatccat atgagagaag aataatacat
    13501 tcagctcttc aaaatgacaa atatgtaatt acatatagtg aaggaaccga tccatataga
    13561 agattagtaa ttgagtgtaa aagataaagt caacctccta aacattaggg ggttttattt
    13621 tttaatatta ttaaatcatt aatggttggt taaatgtaaa tctatatgta aaaaattatg
    13681 aattaagagg tgataaattt gtttatagat gatacaattg cagcaatagc tacagcacct
    13741 ggagaaggtg gtataggtat acttagaata agtggagaaa aagctttgaa ggttgcagaa
    13801 gaaatcttta aatctatgtc tggaaagagt attgaagaat acaataagag aacattaata
    13861 tatggaaata tagtggataa tgaaaatgta attgatgaag ttttattggc atatatgaaa
    13921 ggaccaaatt cctatacagg agaagatgtt atagaaatta attgccatgg tggatttatt
    13981 tcggtaaaga agatattaga attaatacta tcaaaagatg ttagattagc agaggctggt
    14041 gagtttacta aaagagcatt cttaaatgga agaatagatt tatctcaagc agaggctgta
    14101 attgatgtta taaaagccaa aactgatata gctcatgaag ttgctcaaaa tcaattagag
    14161 ggttctttat caaagaagat aagagaattg agagataaag tcactgagat attagctcat
    14221 gtagaagttg ctatagatta tcctgaggaa gatattgagc atataacata tcaaactctt
    14281 aaagaaaaaa ctgatgaact taaaaaagac attaaaaaat tatatgatac agctgaaagt
    14341 ggaaaaatac ttagagaagg cttaaagacg gtaattgttg ggaaaccaaa tgtaggtaaa
    14401 tcttcactac ttaattctat tttaggagag aatagagcaa tagttactga tataccagga
    14461 actactagag atgttataga agagtttgta aacataaaag gtataccttt aaaaattgtt
    14521 gacacagcag gtataagaga tactgatgat attgttgaaa aaataggtgt tgaaaagtct
    14581 aaagaatcat ttactagtgc agatttgata gtaatggttt tagatgcatc tagaaagttg
    14641 tcagaagaag atatagagat attagaaaaa cttaaggata aacaaacaat agtgctgtta
    14701 aataagaatg atttgaaaca agagattgaa gaagaaaaaa tacttaaata tgtagaaaat
    14761 aatagtataa taaaaatatc agctttacaa caagaaggaa tagaagaatt gcaagataaa
    14821 atagagtcta tggtatataa aggtagtatc aaaaataatt ctagtttagt agtaacaaat
    14881 tcaagacata aagatgcact tagtaaagca tataaatctg caacagatgc tttaattgct
    14941 ttggaacaaa gtatgccatt tgactttgta gaagtcgatt taaaaaatat atgggactat
    15001 cttggatata ttaatggtga tacagtaaca gaggatttac tagataatat attccataat
    15061 ttctgtattg gtaagtaatg tacaaaagta aacttgtttt tattatagag atttttaaaa
    15121 aatttgaagt gtttctcatg aaacattaat tttgaaaaga aaggaattat aaatgataaa
    15181 gtttgaagca ggaaaatatg atgttatagt agtcggagca ggacatgctg gatgtgaagc
    15241 tgctttggct actgcaagaa tgggatataa aactttgata ataacaatgt ctttggattc
    15301 catagcatta atgccttgta atccatcaat tggagggact ggtaaaggtc aattagttaa
    15361 agaaatagat gccttaggcg gtcagatggg attaaatata gacaagacat atatacaaag
    15421 tagaatgtta aatactgcca aaggtccagc ggtacactct ttaagagcac aagcagataa
    15481 atttaaatat catgaagaaa tgaaaaagac attagaagat gaaccaaatc ttgatatagc
    15541 tatggatgaa gttgtagaaa tacttcatga aggaaatgtt gtaataggag ttggaactaa
    15601 actaggatgt tcatttaaat ctaaagctgt aatattggcc actggagttt atctgaactc
    15661 aaagatatat atgggtgaag ttgcatttta tgaaggtcct aatgcattag ggtatgcaaa
    15721 atatcttaca gatagcttag ttgagcttgg ccttagaatg agaagattta agacgggaac
    15781 tccagcaaga gttcatagag atagtataga cttctctgta atgtctttac aagagggtga
    15841 tgaaaaagta actccattct catttatgaa tgaaaatata gaaaaaaaac aagaaccttg
    15901 ttatttaaca agaactacag aagaaactca aaaagttata ctggataatt taaaaaggtc
    15961 tgctatgtat agtggagtaa tagaaagtac tggtccaaga tactgtcctt ctatagaaga
    16021 taaagtagta agatttagtg ataaaacatc acatcaatta tttatagaac cagaagggtt
    16081 aaatactaaa gaaatgtata tacaaggaat ttcaactagt ttaccttttg aagttcaatt
    16141 agatatgtac aaaactataa aaggacttga aaattgtaag ataatgagac ctgcatatgc
    16201 tattgaatat gattgtgttg acccaactca actaaaaata agtttggaaa taaagggtgt
    16261 tgagaactta tttagtgcag ggcaatttaa tggaacttct ggatacgagg aagcagcagc
    16321 tcaagggctt atggctggta taaatgcagt tagaaagata gaaggaaaag agccatttgt
    16381 attagaccgt tcagaagctt atataggtgt cttattagat gaccttgtta ctaaaggaac
    16441 taacgagcct tacagaatga tgacatcaag agcagaatat cgactatatt taagacaaga
    16501 taatgcagat atgagattaa ctcaaaaagg ttatgatata ggattagtta aaaaagatag
    16561 atatgaaagg ttcctaaata aaaaggctgc tgtagagaaa gaatttgaaa gactaaaaaa
    16621 tgaaagagtt acaccaaaag aagtaaatag tttgttggaa gaaaaaggag caacaccaat
    16681 aaaggttgga atatctctat atgaattttt aaagagacca gaggttactt acgaattgct
    16741 tgaagaatta gggaaaggcg ctggagaaga tgtttcaaga gaagttaaag aacagtgtgt
    16801 aataataact aaatatgaag gttatataga gaaacaattg aaacaaatag accaatttaa
    16861 aaaacttgag aataaaaagt tggatgagaa aataaattat tcatctatag aaggtcttag
    16921 attagaagct agacagaaat tagatgatat aaaacctatt tcaatagggc aagcatctcg
    16981 tatatctggt gtttctccag ctgatatatc agttttactt atatacctag aacaaatcag
    17041 aagaactaga ggtggcaaag gtgaataata tagaacttct gaaaaacggg attgaaggtt
    17101 ttaatataga tacgaatgac agtatgttag aaaaatttaa aatctataga gagatattag
    17161 ttgaatggaa taaaaagatg aatcttactg gaatagaaga tgaaaaagaa gtgtatataa
    17221 aacatttttt agactctatt tcagctgtaa aaaatggata tataaaaaat gggatgtcta
    17281 taatagatgt tggtacaggt gctggttttc ctggaatacc attgaaaata tgtttagata
    17341 gcttagaact tactttgtta gattcgttaa ataaaagaat aaacttttta gaggaagtta
    17401 gtagagtact agagttagat aatattacat ttatacatgg aagagctgaa gattttggta
    17461 aaaatgaaga ctatagggaa aaatatgatg ttgctacggc aagagctgtt gctggattac
    17521 cgattcttat ggaattttgt gtaccatttg taaaagttgg agggtatttt atttgcttaa
    17581 aaggtccgaa tgctaattta gagctagaag aatcacaaaa ggctattgat gttttaggat
    17641 tggagtatat tgaaaaaata gatgtagagt taccagaggt agatttaaat cataatatat
    17701 tagtttttaa aaaagtgatg gaaacaccag ttaagtaccc tagaaaagct ggtaaaccag
    17761 ccaaaaatcc tattaagtaa tgaaaaattt attattggta agcttaatta tttctcagga
    17821 aataattaag cttactgtaa aaaaacaaaa ataaaggaat tattaaagta gatgcagaaa
    17881 taaatttgta tgtacatata gagaacaaaa ataatttata tctaaaataa gagaggtatg
    17941 ttatggaaga taaaagagtt atggagatac ctatagaaga tgtagttcca aatccatatc
    18001 aaccaagaaa aatattttca caagtttcat tggaggaatt gagcaattct ataaaggtat
    18061 atggtataat tcaacctata acagttagag caaaagatgg caaatatgaa ctaattgcag
    18121 gagaaagaag attaagagca gctaaattag cagagcttaa aaccatacca gcaattataa
    18181 ataatatgaa cgatgaatct tctgctgttc tagcgttatt agaaaatttg caacgagaag
    18241 acttaaattt cattgaagaa gcaattggtt atgaaaactt aataaaagaa catgctttta
    18301 ctcaacagca attagcagaa aaattgggca aaaatcaatc tactgtagca aataagctta
    18361 gaatattaaa gcttccaaac gatataaaaa tgaagctaat agagaataat ttaacagaaa
    18421 gacatgctag agcatttctt aaactaccaa gtgaagattt aatgcaaagt gtgttagaca
    18481 aagtcataaa aaatgaattg actgttaaaa aaacagaaaa attgatacaa gatgtattag
    18541 aagaaactaa agttcaagaa gagcctgata aaaaacagaa tataaagggt gctatgagta
    18601 taagaattta cataaatact ataaagcagg cctttgatgc tatatcaaat acaggaattg
    18661 atgctaagta taatgaaata gataagggag attatatgga agttgttgta aaaatcccta
    18721 aaaaatagag attactgctt accagtaatc tttttttgta atataagcct tgtaatataa
    18781 attgatttat agacattatt tatacttaaa aaacacctat tcaacattta aaattaattt
    18841 aattcttaaa ggggggaaca tcatgggcaa agttatagct gtattcaatc aaaagggtgg
    18901 agtcgggaaa actacaacaa atgtaaattt aagtgctagc ttaggaactt tgggaaagaa
    18961 aatattagta ttagatttag acccacaagg aaatacaact agtggatatg gtattaataa
    19021 aaacgaagta gaaaatacaa tctatgaaat tatgttagat gggttacata ttaaagaagc
    19081 aataataagt actgaatttg agaatgtaga tgtagttcca tcagctactg aactatcagg
    19141 tgcagagata gagcttacat ctaaaactaa tagagagtat attttaaaaa attccataaa
    19201 agcggtaata gatgaatatg attatatatt tttagattgt ccaccctcac tggggatgct
    19261 tacaataaat tgtttaactg ctgtagatag tgttttaatt ccaatacaat gtgaatatta
    19321 tgctttagaa ggtgttagtc aattaatgga gactataaaa ttagtaaaat caagattaaa
    19381 tgctgatata gaaattcaag gtgttgtttt aagtatgttt gatggaagag ctaatttatc
    19441 aatacaagta gttgaagaag ttaagaaata ctttaaaggt agtgtatata caactttaat
    19501 tccaagaaat gtaagacttg cagaagctcc aagtcatgga aaacctgtaa tttattatga
    19561 taaaagatgt agagggtctg tagcttattt ggaactagca gaagaattta tagatttaga
    19621 agaggaggaa tggtagtatg gagaataagt ctaaaagaag taatagatta ggtagaggtc
    19681 ttagtgcatt gataccagaa attaaaggag aaacttcaga aaaagaaata gtcaacatag
    19741 atatagacaa aatatatcca aacgaagttc aacctagaaa gcaatttgat gaagaaaaga
    19801 taaaagtatt gtcggattct ataaaaaatt atggtgtttt acagccaata gtggttaaaa
    19861 tggatgaaaa taataaatat atgataatag ctggagaaag aagatttaga gcatcaaaac
    19921 ttgcaaataa aaatcaaata cctgctataa ttaaagatat agatatgaaa gatataatgg
    19981 agatagcctt aattgaaaac ttacagaggg aagatttaaa ttcaatagaa gaggctctag
    20041 catataagag tttaatagag cattacaatg taactcagga agaaatatca gaagctgttg
    20101 ggaagagtag acctcatata actaatacac taagactttt aaatcttgga caagatgtta
    20161 tagaaatgat agacagtggt agaattacag caggccatgg taaggcttta ttgagaattg
    20221 cagataaaga tttgcaactt caaatagcga aaaaaataga agaagaagaa ctttctgtaa
    20281 gagaagtcga aaatatagct aaaaaaatat ctgaaaataa acaagaagaa cctaaaaaat
    20341 ctaaacctaa ggatgtattt attttagatg ttgaagataa acttagaaat atatttggaa
    20401 caaaggtgaa tatttctaaa ggaaagaaaa aaggtaaaat agagatagaa tactataatg
    20461 atgatgattt aaatagtatt gtatcaatgc ttcttgaata gaacaaaaat ataaaacaga
    20521 gaggaagatt gttatgattt atttagataa tgctgcaact acatatccaa aaccagaaag
    20581 agtgtataat gctgttttag attgtatgaa aaattattgt gcaaatccag gtagagcagg
    20641 ccataaactt gctatgagag ctgctaggga gatatatgat acaagagaga atatagctaa
    20701 gttgttcaat gtaagtaatc ctatgaatat agtatttaca tctaatgcaa cagattcttt
    20761 aaatttagcg ataaaaggtg tattacaaga aggagaccat gtaataacaa cgtctatgga
    20821 gcataattca gtaataagac ctataaaagc attggaaaaa aggggtattg aaaatactgt
    20881 agtaaaatgc gattatgagg gatttttaga ttatgaagat ttagaaaaat caataaagaa
    20941 taatactaaa ttgattgtaa caacacatgc gtctaatgta tgtggaactt taatagatat
    21001 aaaaaaagtt ggagaaatag ctaaaaaaca taatatacta tttttagtag atgcatcaca
    21061 aacagcaggt gtttatgata tagacgtaaa tgaatgtaat atagatatgt tggcaatgcc
    21121 aggtcataaa tgcttatttg gacctcaagg aacaggtata ttatatgtaa gagaagggtt
    21181 gaacttaaat atattaaaag aaggtggaac aggtagtaaa tcagaagaaa tagttcaacc
    21241 agagttattc ccagataaat atgaatcagg aactcataat actccaggta tagcaggtct
    21301 taaccaaggt atactattta tatttgaaag aggaatcaac aatataagac aacatgaaga
    21361 agaattatgt caatatatga tagacaaatt agaggaagta ccagatataa agatatatgg
    21421 accaaaggac agtaaaaaaa gagcatcagt aatagctcta aatataggag atatggattc
    21481 tggagaggtt actttcttat tagatagtga ttataatata gcgactagat caggaataca
    21541 ttgttcacca ctagcacata ccactttagg tactttaaaa caaggtgcag taaggttcag
    21601 tataggatat ttcaacacaa aagatgagat agataaagca gtggaagctt tgaaaaaaat
    21661 ttcaaaaaat aaatagcatg agatttaata atatagaaat aaaaaaaggg gatatttcaa
    21721 aataaaaatt tgaatcatct ccttttttgt acaaaaaaga aaaggggttc ctaagaacct
    21781 ctaatctatt tgataatagt ttcaagaaat tactttaagt aatctccttc ttcaagaact
    21841 atatgaccac tttcgtaatt agaaccatca acagtaattt ttatttgttc aacattcata
    21901 ttttttatgt aagtacgagc aactgaatca agcattaaag tttctgcatc actacctaag
    21961 tttgactttg taaagtcaga gctaacatct aatataccag tttttacacc atcaatgtct
    22021 tttgtagtaa atgaatttaa ttttgcagat tctggaacaa ctttaagttt ttgaagttct
    22081 tcaaataaag tgttctcatc aattttattt aaatcaactt tattttcaat taaatctgtc
    22141 ttatctacat caaaagagta aatagtagct gattgagctt tagtttcttc ttttggagta
    22201 ggtttagctt tgttattagg aacctctttt tcagtattgt tttcttcttt tggttgctca
    22261 acatttgtat tgctcttagt ttctttttta gatgaatcac ttccattttg agcattagta
    22321 caaccaactg cgaatataga tatagataat atacttaaca ctaacattat tttcttattt
    22381 ttcataaaaa tttctcctct cttaaaaact attcttatat ataattatag tcgttatgag
    22441 caaatatatg aatacaaaat agtaacaaat ttaaaaaatg atttatttaa attaataaaa
    22501 ctcaaaataa actaaatttt agtcattttt atgggaaatc atttaatgta cagtgaaaat
    22561 attgtaaaat aaaagtagtg tttttaaaat aaatataagt tataattata ttataaaaaa
    22621 atgtcttcaa aaggtgggaa tagtatggaa attttgagct taggagaaaa gattaaaaag
    22681 ttaagaaaag aaaaaaacat gacattgaaa gaattagctg gagataggat aacagcagca
    22741 caaataagcc atatagagag agataaatct catactagtt atgaactgtt ggaatatcta
    22801 ggcgataagc tagatgtaag tgtagaatac ttattagaga ccaaggaaat gcaatcaaaa
    22861 aagataactg ataatttaat actacagagc gaaatttata taaaagaaaa tgaattaaac
    22921 aaggctgagg accaaataaa agaagtttta gatatatgta agagatacga tttaatggag
    22981 aactatggca gatgtaattt tttaatggct aatattaatt tgaaaaaatc taattataat
    23041 gatgctatag aaaactttga aaaggcattg tacttcttta taaaaaatag tgatagtgaa
    23101 aatatactta agtgttattt aaacataggg aaaatatata tgcaggaaga cttttataaa
    23161 ggtgctataa gccactttaa ttttgcagag gaaattttgt cagagagtaa atttgaggat
    23221 gttaacatac acaaggaact atatagtaag atggcatatt gttacataaa actagataat
    23281 ccaaatatgt cattgaagta catagaaaag ataaatgaaa tagataatag aaatgataga
    23341 aaagaagatg tcgatatttt ggtgttaaaa gcaaataata tgcttgagat tggaaaatat
    23401 gaagaatcaa gagattactt taaaaaagca ctaaaaatac ttgagagtga ggataataaa
    23461 actggattag caaatgtgta tttaacaatt tgtgatgttt acagaaaaat aggtgaaaca
    23521 gatagagtac ttgaatattc acaaaaggta tatgacataa agaaaaatga tgaagatgaa
    23581 tacatgatgt caggattatt taagataata gaagcatata tagataaaga agattatgat
    23641 ttagctagaa aatattgtaa gatagcctta gcttcatcaa taaaaaataa aaataaattc
    23701 aatgaatata aagcattgag attctatgct aatatgtata aaaatcagaa tgaagtaact
    23761 cttgcaattg aatatctgat taaatgcata aaaatagtat cagatttagg caacaataga
    23821 atattggcca atttatatat agacttaggt caattatatt caaatatatc aaaagaaaaa
    23881 gagttagaat attatcaaaa aggtgtattt atgtacaaaa atttagaaat aatgtagggt
    23941 aaataaacct ataagggtat agaaacaatc tataacaagc aagataatat ctatgataaa
    24001 atataaattg tatgaatatt tttaaggaga tgaatcagac aatgagtata aaaatagatg
    24061 cgagagggct tgcttgtcct aaaccagtta taaatacaaa aagagaattg gataacctag
    24121 aagaaggtgt tgtagttaca atagttgata atgaaacagc taaggagaat atattaaaac
    24181 ttgctaaatc tttaagttgt gaagcaaata ttgttgacga aaaagagaat tttatatcta
    24241 tagaaattag aaaaggtaaa aatatactag ataaacaaaa tgttgtagat aataaagaaa
    24301 gtgaattaga caatacatgt atatttatat cttcagataa gatgggactt ggaaatgacg
    24361 aattaggaaa agtgttaata aaaggattta tatatacatt aacagaatct aaaccttatc
    24421 ctaagtatgt acttttagtt aatggaggag taaaattaag tgctgaaaat gaagaaacca
    24481 tagaaaatct aaaaatactt gagaatatgg gtgtagaagt tttatcttgt ggtacttgtt
    24541 tagattatta taatttaaaa gacaaattac aagtaggtac agttacaaat atgtatacta
    24601 tagttgagac attaaagaat gcatcaaata ccatttctat ataatgtatg tatgtttaat
    24661 aatataaaac taacaataaa gattaactca aatagatagt cagaaaggat taagtaatga
    24721 atcaaatgta tatagtgtca tttaattcga cacatcatgc gataagatca gaaaagttat
    24781 ttggtgaaaa tagtctaaaa gtaatggctc ttccaacacc aagagaaata acagcaagtt
    24841 gtggaatatc aattaaattt tcttttgaag atatggaaaa gataaaaact attttggttg
    24901 aaaataatgt agatataaaa ggaatttatt gtataagtaa attagaaaat ggttcaaaag
    24961 aagtagagaa gttagattag gaggctaatt atttatgcca atggatttaa aaataggtga
    25021 tattgttgaa ttaaaaaagc aacatgcatg tgggtgtaaa gagtttgaaa tcataagaac
    25081 gggaatggat ataaagataa aatgtactaa atgttcaagg ctaataatgc ttgatagaga
    25141 gaccttagaa aagagagtaa aaaagctaat aaaaaagtga taaaaattta acacttaaaa
    25201 aatgtcaaat accgtatatt aatatccaaa attttggtat aataagaatt gaagaaaaca
    25261 ttttcatggg ggtgctttaa ttatggcagt taaatatgca aaaagaatgc aaggattaca
    25321 aggatcagaa atacgtgagc ttttaaaact tactcaacaa ccacaaataa tatcttttgc
    25381 tggtggaatg ccggctccag agttatttcc agttgaggaa atgaaaaaag tatcagttgc
    25441 agtgcttgaa gaaaatggaa gatcagctat gcaatatact acaacagaag gatatgaacc
    25501 attaagagaa aaaatagcag ctagaatgaa tgataaaaac aaaactaatg taaacaaaga
    25561 tgatatatta gttacaagtg gttctcaaca aggtctagat tttgcaggaa aagtatttat
    25621 tgatgagggt gatgtaattt tatgtgaaag tccatcttac ataggagcta taaacgcatt
    25681 taaatcttat caacctaaat tcatagatgt tccaacagat tctgatggaa tgataatgga
    25741 agaattagaa aagatactag aaacaactga tagaataaaa atgatttatg taatacctga
    25801 cttccaaaat ccaacaggaa gaacttggcc acttgaaaga cgtaaaaaat tcatggaaat
    25861 agtaaataaa tttgaaatac cagtaataga agataatcca tatggagatt taagatttga
    25921 aggagagact ttaccatctt taaaatcaat ggatacaaaa ggattagtaa tattcttagg
    25981 aactttctct aaaatattct gcccaggata cagattagga tggacttgtg cttctccaga
    26041 aattttatct aagtttaact ttgcaaaaca aggtgcagac ttacaagcat caactatatc
    26101 tcaaatggaa gtaagcaaat ttatggatat gtatgaccta gatgcacatg ttgacaaaat
    26161 aaaagcagtt tatgttaaac gtagagatgt aatgcttaag actatggaag aagagttccc
    26221 agaaggatta gtatttactc atccagaagg tggattattt acatgggtag agcttccaag
    26281 taatttaaat gctaaagaat taatgccaaa atgtttagat aagaatgttg cttatgttgc
    26341 tggaggagga ttcttcccta atggtggtag agaaaatact ttcagactta actattcaaa
    26401 tatgccagaa gaaaaaataa tagaaggtat aaaaaatata gcagctgtat taaaagaagc
    26461 tatgggtgta gaagcttaat taaaaataat aacttatata taatacaagg gatggttgtt
    26521 gccatccctt gtttgtttga aaagtatata attcacctta tactaaactt tgtccaaaat
    26581 ttaaattgac agaaataaga ataaatgata aaatgtgtag gtatgagatt ttagtctcat
    26641 ttctctgctc taagagtgta gagccgttaa gtccaaaggg aggtgaatta tagtgagaaa
    26701 ttatgaatta gtttatgtgg taaagccaaa ctctgatgaa gaagtaagag aggctatact
    26761 aaacaaagtt aaggaagttg ttgcaactga cggagaaata gttaaagttg atacttgggg
    26821 aactaaaaaa ttagcttatc caatagctaa gtttacagaa ggtttctatg tattagttaa
    26881 cttcaaatca gcagtagacg ttcctaaaga gatagacaga aacttaaaga taaatgaaaa
    26941 cgtaataaga cacatgatag ttgttgctta ataactggtt ttattataaa aaataggtgg
    27001 tgtttttatg aatcaagttg tattagtggg aaggttgact agagaccctg aattaagata
    27061 catcccaggt acaggaacac cagtggcatc ctttacaata gccattgata gagactatgt
    27121 aaaaaaagat ggatctaaag aaactgattt tatacctgtt gaagtaatgg gtaaatctgc
    27181 tgaattttgt gctaactaca taactaaagg tagattagta gcacttcaag gatctataag
    27241 agtagacaat tatcagacac aatcaggtga aaagagaact tttacaaaag taagtactag
    27301 atcagtacaa gctttggata gtaagaataa atcggaaaat tcatatagag agagtgctcc
    27361 agcttttgag ccaagctttg aacctcaagg tttagaccca caaggtttcc aagctataga
    27421 tgatgacgat ataccatttt aaaaaggagg gaaaaaagtc atgatgaata aaaaaagacg
    27481 taagaaaaaa agagtttgtc aattttgtgc tgacaaaaac gctaaaatag attacaaaag
    27541 tactcaaaga ttacaaaagt atataactga aagaggaaag atattaccaa gaagaatatc
    27601 tggaacttgt gctaagcatc aaagagaatt aacagttgct ataaaaagag cgagaaatat
    27661 agcactttta ccatatacat tagactaaaa aaatgagcct tgtgctcatt ttttattata
    27721 tgtatttata tatctaaatg aggcgagcta taaagctcgt ttttttgttt atatacttaa
    27781 atatattatt atttatgata taatattata tattaaggag ggttgtaaac gtgaaaatag
    27841 ataaaggttc tgagataacg agaaacataa agataataga atggatgaaa acagaaatat
    27901 taatgagtgt aagtgatttg tttaatttat tgtttaaagg ggtaaaacca ttagatgaag
    27961 caattcaaga tactttagca aatataataa tgataacata tctcttagca aagagattag
    28021 gaattagttt tagagatgtg gattataaag tcaaggaaaa aataaaaatt ggtatagatg
    28081 aggaccatag cgttgaaaga tggtatggag atttatctaa tttaaaaaaa catatggata
    28141 atagggagtg atattaaatt gaataataaa attagactat ctaaggcaat gtctataata
    28201 gttttagcta taataatcgc tttagtaata gcctatattc caatgctagg aatatttagt
    28261 atgttagcag ctgttccata tgtaattata ggtgctatta ctgatagaaa gtattctctt
    28321 atatctattt tggttacatt ttgtgtactg attctatttg cagacatttc ttatgcatta
    28381 aatatatgta taatgtattc tttaccaggt atagtaatag gaaagatgct aaagagaagc
    28441 tttgaacaag aggacagtaa taaattcgaa ccgatatatg gtggaaccat aatatttgta
    28501 ctttcaatgc ttgtatattt ttttgtatta aagacatttt taaatgtgaa tctattagat
    28561 gaagtttcta agatgatatc agaaattgta aatattcaaa aaaatagttt ttctgcaact
    28621 gaacttaaag tgtttgataa aatgaagcca gatgagattg taagttattt tacaaatatg
    28681 ctaccaatga tgttattttt acaagggttg ttatcagcgt ttgtcacata ttatttgagt
    28741 gtatttttta taaaaaggat tacaaagatg aatataagat ttcctaagtt tgctgatttt
    28801 tatttaccgg gaaatgctat actcacaact ttcttacttt atttattagt cttatttatt
    28861 caaattattg gttcaaaatt atatacagaa ttaataatga ctaatttaca gctagttttt
    28921 aatttaatgt ttgtaattca aggtatagca gtttgcattt atttcttaaa gaaatggata
    28981 agacagggac caaataaaat tatgtttttt tcagggataa ttttatgttt atttggattt
    29041 atgggaatat cttttgttgg tatggtagat agtataattg actttagaaa ggtaagaagc
    29101 tataaatcca cttaggagga tagaaatgag taataaacaa acctttaaat taaatatgcc
    29161 agaaataaat ttatatataa ttgttattgg tatttctagt ataattttgc tttattacaa
    29221 cttgtatgta gggtgtttat ttttctgtat ttttgtatat atggtttttc acaattggag
    29281 aactactaat attagacgtc atgaatggac agagtatatt caaaatttat ctttagatat
    29341 agatgaaaca actaaaaaag ccataataaa tcttccaata ccattatgta ttttagaatt
    29401 tgatggaaat atatcctggt ataatggaaa gttttatgat atgattggtc aaaaagattt
    29461 acttgataaa aatatagagg atatagttaa aaatcttaat ttaagaaaag tattaaatga
    29521 aaataaagag atgtacacag aaataaacta taaagaaaaa gaatatacaa taatttataa
    29581 tgttataaaa aatgatcaag aaaaaaatcc taaatatttg atgatattat actggataga
    29641 caagactgaa tatttaaaag taaaacaaaa ttatgatgat gaaaaaaatg caatgatgct
    29701 aatacaagta gatggatatg atgaagtatt aaaaagtgca gcagaagata agagagcttt
    29761 aattaatgtt gaagtagaga aaatactttc tgctttagaa ttaaattcaa atggtgcatt
    29821 gagaagaact tcaaaagaca aattcttctt ggtaatgcat aaaaaagagt taaaaaaatt
    29881 ggaagctgag aagttttcta ttttagatac aataagacat attgattatg gaaataatct
    29941 tcctgttact ataagtatag gaattggtat agatggagat acattaaatg agaacctaaa
    30001 acttgctacg ggtgctctcg atttagcact tggtagaggt ggtgaccagg ctgttgtaaa
    30061 aacaaaagat aaatttgtgt tttatggagg aaaatctaaa gctgtagaaa agaaaaccaa
    30121 agtaaaatcc agattaatag gtcatgcttt aagagaggtt atacaacaaa gtgaccaagt
    30181 ttatataatg ggacataaat atcctgatat ggatgctatg ggagcagcag ttggtgttta
    30241 tgatatatgt aaatcctgta acaaaactgc aaacatagtt ttacaatctg taaatgaatc
    30301 tatagaaata tttataaata aaataaatga aaataattac tataaaaagt tgtttattgg
    30361 aaaagaggaa gctattgaca actgcactaa aaatacatta gttgtagtag ttgatactca
    30421 tagaccaaat tatactgaat gtgaagaact gttaaagcta tcagagaagg tagtagttat
    30481 agaccatcat agaagaggtg tagagtttat aaatgatgca gtgcttttat tccatgagat
    30541 atatgtatcc tcaacgtgtg aaatggttac agagttagtt cagtatatgg atgaagatgt
    30601 aacaataaat aaacttacag ctgaagggct tttagcaggt ataagtctag atactaaaaa
    30661 ctttgcattc aaaacaggtg ttagaacttt tgaagctgct tcttacctta gaaaagttgg
    30721 agcagatact atagaggtaa agaaattctt caattctgat gtaaaagatt tcataataaa
    30781 agctgaaata attcaaagta ctaaaataat taataataga atatgtttag catattcaag
    30841 tactgagata gatagtataa atgttataat agctcaaact gctgatgagt tattaaatat
    30901 caaagaagta gaagcttcct ttgtattagg tgaaaaagat gatactatat ttataagtgc
    30961 gaggtcttta ggacaaataa atgtacatgt acttatggaa aaattaggag gcgggggaca
    31021 catagatata gctggagcac agttaaaaaa tgtttctcta aaagaagctt ataagatggt
    31081 aaataaaata atagaggagt atttggagga ggaagaatag atgaaagtta tattattaaa
    31141 agacgtaaaa ggaactggta aaaagggaga aatgaaagaa gtaagtgatg gatatgcaag
    31201 aaatttctta tttcctaaaa agatggctgt gcaagcagat agtgtagcaa taaaagaatt
    31261 aaatgaaaag aataaatcta aagaaataaa agcacaaaaa gagtatgaag aagcagtttt
    31321 attaggtaag caaatggaag aaattaatat agaaatatat tcaaaagcag gagaaggtgg
    31381 aagattattt ggttcaataa cttctaaaga aattgctgag caattaaaga aacaaaaaga
    31441 catagatgta gataaaagaa aaatattatt agatgaacca ataagatctt taggttcaac
    31501 tttcgtagaa ataaaaatac atcaaaaagt aactactaaa ataagagtgg atgtaaaaga
    31561 aaaacagtaa taactagagg tgattaagat ggaagatatg acgagaattc ctcctcatag
    31621 tgtagaatca gaacaatcaa tattgggttc tatacttcta gataaagatg ctataataac
    31681 ggttacagaa actataaagc ctgatgattt ctataaagag gctcacaaaa taatatatga
    31741 atgtatgata actttaagta ataaaggtga gcctatagac ttgataacat taacagaaga
    31801 actaagaaaa caagggcatc ttaatgatat tggaggaatc agttatatta caagtctttc
    31861 tactatagta ccgacgactt caaatgtaaa atactatgct gatatagtaa aagaaaagtc
    31921 tgtacttaga aagcttataa aagcttctaa tgaaataata aatctaggat atagtggagc
    31981 aactaagatt gaagatgttt tagaacaagc tgaaaagagt atatttgata tatcacagga
    32041 gaaaactagt gatgatttta aatctataaa cttagttttg atggatgcct atgatatgat
    32101 agaaaaatta tacactaaca agagtgatgt cactggtata actactggtt ttaaagattt
    32161 aaacaaaaaa ataaatggtc ttcaaaggac tgatttaata ttaattgcag ctagaccagc
    32221 aatgggtaaa actgcctttt ctttaaactt ggttcaaaat gcagccttaa aaggtgatgc
    32281 ctctgtagct gtatttagtt tagagatgtc taaggaacaa ttagtacaac gtatgttatc
    32341 ttcacaatct agtgtagaat taaaaaaaat aaagactgga acacttaatg ataatgattg
    32401 gcctagaatt atagatgcta tggcagtatt atcagatgcc aaaatacaca tagatgatac
    32461 tcctggtata aaaatatcag aactaaggtc aaagtgtaga aagttaaaaa tagaaaaagg
    32521 actagattta gtgcttattg attatcttca acttatggaa ggtgaaggta ataatgaaag
    32581 cagacaacaa gagatatcta agatatcaag gtcactaaaa atattagcaa aagaattaaa
    32641 ctgtcctgta gtagcactat ctcagttatc acgtgcacca gaacaaagag cagaccatag
    32701 acctatgtta tctgatttaa gagagtctgg agcaatagaa caggatgcag atatagttat
    32761 gttcttatac agggacgaat attatcatgc tgattctgag agtaaaaata taggagaagt
    32821 aataatagca aaaaatagac acggtgaaac tggttctgtg gaacttgttt ggcttggtga
    32881 agttcaaaga tttggggata agttgagaga cctataatct aacgaatatc cacagaacgt
    32941 cctttctttc tacaggaaaa atgtggatac atgtcactaa atatgtccaa acattgaaat
    33001 ttagctaaaa aactatccac actatccaca gatggcctgt tgataagtgt gaataagtag
    33061 ataaagcaag cttttggctt gctttatctg tgtataaata ttaattatta ggaggaataa
    33121 tatggaaaaa ataagcttaa aatatatata tcccaatata attaaagtac ttgatgaaat
    33181 aaacttattt agagtaattg acaataattt aagagaatct attgttgttt atgctaataa
    33241 tgtggataat cagtatcata taaacatgac taatacaaac tttggaaata taattaacat
    33301 atgtaaatta gaaaaacttt tagatgtgga taaatttatg gaaaaagtta taaaatatga
    33361 aaaagaaata atagagaaag aagagttttc aaaaatagaa gaatatatgt taaatattgg
    33421 agaatactaa aaaagttgaa aaaaatacga atgtttgata gaataagtaa gtaaagtgtt
    33481 cgagagtata taaaatatag aggtgaatta catgaaaaca gttgcaattg ttggttctca
    33541 atggggagat gaaggtaaag gtaaagttat agattatctt gctactcaag cagatgtagt
    33601 agtaagaggg caaggtggaa ataatgcagg acatacatta gttgtagaag gtaaaaaata
    33661 cgcattacat ctaattccat ctggagtttt aaatccaaat acagtgaata taatcggaaa
    33721 tggaatagtg tttgacccta aaggattttt agaagaattg gaaatgttta aaacggataa
    33781 tataagtaca gaaaatataa aaataagtga tagagcacat gtcatattcc catatcataa
    33841 agaactagat gcattgtcag aagaagccag aggcgattta aagataggta caacaaaaaa
    33901 aggtattgga ccttgttata tggataaaac agagagatct ggtattagaa tatgtgactt
    33961 aatggataaa gataaatttg ctataaaatt aaaagctcaa atagatgcta aaaatgaaat
    34021 agttaaaaat atatatggaa aagaagaatt atttgatttt gaaactatat ataacgaata
    34081 tctaggatat gcagagcaaa taagaaaata tgtggcagat acgtctgtta ttgtatatga
    34141 tgcagtaaga gcaggtaaaa aagtattgtt tgaaggagca caagggactt tattagattt
    34201 agatttagga acttatccat ttgttacatc ttctcatcca acatctggtg gatttgcaat
    34261 tggtgcaggt atagggccaa atatgataaa agacgttgtt ggtatagtaa aagcatatac
    34321 tacaagagtt ggagaaggac cgtttgttac agagcaaata aatgaaactg gagataagat
    34381 aagagaacaa ggacatgaat ttggggtaac tactggtaga ccaagaagat gtgggtggtt
    34441 tgatgcagtt atagttaagt atgcagctag agttaatgga ttgactagta tttcatttat
    34501 gttacttgat gtattaactg gatttgataa gataaaagtt tgtacgtctt ataaaatggg
    34561 cgataagata ataacggatt tcccagcttc attagatgat ttagcaaaat gtgaacctgt
    34621 atatgaagaa ttagatggat ggaatgaaga tataactcaa atagataact ttgatgacct
    34681 tccagaaaat gcaaagaaat atgtagcaaa aatagaagaa ctagttggag taagtgtaga
    34741 tatggtttct gttggtccaa atagagctca aactataata agaagaaata tatttgctta
    34801 atattttatc aattaaataa tttaaaatag ttgaagaaga gtatctcaag ataatgttta
    34861 tagttattag agatgctctt ttatatttga cttacattga ttttttatta agttaaagct
    34921 acttatacat attctaactg aaattaaagt tgaatttatg tagatacata taggtttttt
    34981 aaaaagaatt ttgttgaaaa taaatggcta aagatgattt taattatatt ctaaaaaaag
    35041 taaaaaaaac tattgactaa aaattatata gacgctataa tataacttgt gagtgaaaga
    35101 aacgacgaaa aaagtcgaaa aaatgtggtc tattagctca gtcggtagag cacctgactt
    35161 ttaatcaggg tgtcccgcgt tcgagtcgcg gatagatcac caaaatatgg ctcgttggtc
    35221 aagaggttaa gacaccgccc tttcacggcg gtaacagggg ttcaattccc ctacgagtca
    35281 ccagtatggg actatagctc agctgggaga gcacctgcct tacaagcagg gggtcacagg
    35341 ttcgagccct gttagtccca ccaaattttg aattagagat aaatactaga ttatatgctg
    35401 gtgtggctca acggtagagc agctgacttg taatcagcag gttgtaggtt cgattcctat
    35461 caccagctcc acaaagtaca taaattcaaa ttaatataat tttctatgtt acaggatact
    35521 tttaagtatc tttttttaat ttcattttta ttgtattaaa aatgaaatta aacatacaac
    35581 aaaattttga attacttgca atgtgatagt tttaaatata aaatttagaa tcattctagc
    35641 ataatattat gtaatatagc aaatttaatt tatagtatca ataaatatag actactaaga
    35701 atgtaaatga tttgttagat taaggtacat tagatatgta aatagaaaaa taaaaagtat
    35761 gttttaattt atgtgatata agttttaaac agagaagcaa gtttgctctt acctctctat
    35821 ttaaaaataa aaaaatgttt tttaagggct attttattta aatctttccc atctcaaatc
    35881 atctccagta aattctaaaa tcctaaactt atctaatatt ctagagagag ttctttgagt
    35941 gtaagtattt cctatttgag atggtgttaa gttggttgaa ataatgattt ttttaccagc
    36001 aacaagtctt gtatttacaa tattgaagat ttctccacta gtaaaagaat tattaagttc
    36061 agttccaagg tcatctatta ttaataaatc acaatcaaac aagtttttat aattatcttc
    36121 acttatttga ttatttgtgt ctcttctaaa tttataatct tcaagaatat ctaaaattct
    36181 aaaagaagtt tgatagatta caacattgcc tttatctaac aattcctttg caatacaatt
    36241 gcacatatag gtctttccta gtccagtaga accataaaac agtagatttt cactattatc
    36301 cttcttaaaa tcatgaacaa aattttcgca gatacttaaa ttatttagca tattttctct
    36361 aggagatatt tcaccatcag agcctttctt aggagaaaat atattcaagt taaaattcga
    36421 aaaattttcc tgacttaaaa tcctatctaa attagacatt ttataagctt catttacaat
    36481 ttcttgtttg aggcaagaac atttttctcc attaggtaaa aatcctttat ctttacaaga
    36541 aatacattga aactttattt ccaagtaatc aagaggtatg ttactttcag ctaaaagtct
    36601 ttctttcttt acttttaaag attctatatt ctctttagtc ttctttacaa tttcatcttt
    36661 agacttagga tttaaaagta ctatcttagc taaactgaga cctattttag aaatttcatc
    36721 atctatagat tttatctcag ggattctatt atatacctca ttttttctat gttctaataa
    36781 gagttcatta tcatctcttc tcttagcata tttagcaagt atttttctta ttttatcctc
    36841 attcatttaa tcacctactt aaacttttct ttttgacttt ttttaataat ctcatcgagc
    36901 tcatcagagg tatactgagt gaatgtttcg ttgaaattat gaaacttagt ttttttgtaa
    36961 gtattgttgt tttgagtagt attaatctgc ttcttattaa tattctcctt aactatacgt
    37021 tcttcctctt tttgctttaa atcatttaaa ttttttatat ttttttcatt ccaattttta
    37081 attataccat ttatataaga tatacttggg tttgaaatat tcttagactt agaacatgca
    37141 tttattatca aatctatatc catattaaat ttatcaaacc aggtgtccat aagttctttt
    37201 tctgattttg atggttgtct tgaaaatccc aattcattga aaatcgtttt atataaaata
    37261 tatcgttcac ttcttaccaa aaagctttct tcaacatctt ttggcgtata taaatttgaa
    37321 tcataccaat ttctaataat gccttcaatg tatttaactg gctttgatgt accagtttta
    37381 tctttgacgt attcatatgc gcaaagtatc atatcggggc tcatattgta tttattcatt
    37441 atatccataa tactcatctt ttcacttggg tcgagatatc gcccaactat tttatttata
    37501 gaattaaaca tttttctaat acttggattt tcagaagtag atacaacttt atctgtatta
    37561 gattttatag atgaattatt gcttaatata ttctcaatat agaaattttt taaatctaaa
    37621 aattctattg aataattaaa attatcatat tcttcattgt catgtatttt tataatcttc
    37681 ttttcttccc agaatttcca agcagataac acatcagaca atggtatatt taggtttttg
    37741 gctattgaat tattgtcgaa cttaggatta gaagtaatat cacatgcttg tctatatcct
    37801 aaaagataaa cttttacgta taacccgtcc gccattggca taaatatatc tataaagata
    37861 ttgggaatag tgatttcacc taaatctatt tcattgcttt ctttaaaaaa cattttatca
    37921 cctcagatta aattatatca ttttttatat tatatttgtc tgtaaacaaa taaaaggtca
    37981 caagattagc atgtaaatat tttctaaaag tattcatttt gaaaaggagg agatattttg
    38041 gttataaaat tcaaccaaaa agtaagaaga acattatcag tagcgattct atttatggta
    38101 ttaataatag gttgctttat agaaaaggat gacagtttag aaacatacaa agaaaaaact
    38161 aattcaaaaa taaatcagta taatatggat gttatatttg atgatgagac taagaggtta
    38221 atgtgcaatc agaatgttga ctacataaat aatacaaaat caaacataga taaaatatat
    38281 ttccatatat atccgaatgc tttttccaag aaagattttg ctccttttga aaaaagtgaa
    38341 atgacaaggg cttatcctaa tggatttaac gaaggatata tagatataaa gaatatatta
    38401 aataataata gtaaaatgga gtataaaatt aaaggagaca aaaatgacat attagaaata
    38461 gaattaggga aagagttaaa accaaatgaa agaatgtcat tagatataaa atataacgta
    38521 aaaataccaa attcaattgg aagatttgga tatggagaaa atactataaa tgttactaat
    38581 tggtttccta tagcttgtgt aaatgatgaa aggggatgga atttaaagag ttatgaaact
    38641 attggcgacc cattttatag tgagactagc gattttcatg tgaaactatt gattccaaat
    38701 aaatataaaa ttgctcatac aggaaaactt attgatgata aacatgataa taagaaaatg
    38761 ctatatgaaa tagaagctga aaacgttaga gattttgcat ttatactaag tagtaaattt
    38821 gatgtaaaca aagtagattc aaagggagta gctatcaata catataattt aaataaaaag
    38881 ctttctaaaa aagcaactga agtggcaaaa gattcaataa atatatttag tgaattattt
    38941 ggaaagtatc cttacaaaga attttctgtt gtagcaagtg acttttttat aggggggatg
    39001 gaatacccta tgcttgttat gatagaccaa agtttatata atgaaaaaaa tgaattttta
    39061 ctagaatatg ttattgccca tgagacagca catcaatggt ggtattctgc tgttggaaat
    39121 gatgaaataa gtgaaccctg gttagatgaa gcacttactg aatattctac aatagtttat
    39181 tttgaacaaa agtatggtaa agatatggca aacaagctta taaaaactat ggagatacag
    39241 acaaaaagtt atttgagtga aaatatattt aagccagcaa atgagtataa aaattctact
    39301 gaatacagct tgaatgttta tacaaaaggc gcaatagcat ttaatgaaat aagaaaagaa
    39361 gttggagata aagtattctt tgagacttta aatgagtact ataataaata taagcataaa
    39421 aatgctaatg gaagagcttt tgtggaacta tggaatagta aaggtgtaga tataaataag
    39481 attataagtg aatgtaagta aatagtgaat gtaagtaaat tttgtgcaga gtattaaatt
    39541 ttatttaata ctctgttttt ttggtataat ataatttata aggcagaaaa agaggtgaag
    39601 gaatgctaaa gtattgttca gtagggagtg gtagtagtgg taattgccac tatatagggt
    39661 ataaaaatac taatatacta gtagatgcag gattaagtgg caagagaata actacaggtc
    39721 ttaaagacat tggtgtagat gcagataaat taaaaggaat atttataaca catgagcatg
    39781 ttgaccatat aaaaggagca ggtatattat caagaaagtt tgatatacca gtatttgcta
    39841 atataaaaac atggtgttct atgaaggaca aattaggtga catcaaagat aaaaatatga
    39901 aagtatttga aaatgataaa acctactcat taggtgatat catagtaaga cctttttcaa
    39961 tagaacatga tgcgtctgac ccagttggat ataattttta tacagagagt gatgaaaaaa
    40021 tgtctatagc tactgatata ggatgtataa ctcaaaatat aaaaaaacat ctatataaat
    40081 ctaaattagt agtattggaa tcaaattatg accctaatat gcttatgatg ggttcatata
    40141 catatgcttt aaagaaaaga gtaatgtcaa gtactggtca tttatccaat gaagatgcag
    40201 ctaatttctg cgttgaactt attaatgaag gtacagaatc tatattatta gcacatttaa
    40261 gcaaagaaaa taattttcct gagttagcgt atgagacttc aaaaggagta ttagcatcaa
    40321 atgatatagt agttggacaa gatgtaaaac tagatgtact atcacgaaat gatgtttcta
    40381 atgtttatga gatgaagtag aggtgaataa ctttgctaaa gagaaatttt gaaaatgttg
    40441 aggataataa aattttatac tctataaagc agggtatgat tttagcgata cctgctatta
    40501 tgactggttc aactgctctt gtaattttga atcttccaat aaaggcttat caagaatatt
    40561 tatcaggctt gtttaatgga gaggttacaa atattcttaa ctttataaat gattctactt
    40621 taggaattat ttcattaata atattattaa caataagcta tagttatgga aaaatatatg
    40681 ggagcaagta tacagtactt gttcctatag ttgcaatgtg ctctttttta gtattttcac
    40741 atggtaatga gcttaattct tatatagaaa tatttaaaac aaagtggtta tttacatcta
    40801 taattgtttc tatgacatct tctgtattgt ttgtaaaact tacagaatct tttgttacaa
    40861 gtgtaaagtt tcatacagaa ggggctgatg cagactttaa tatggtagtt tcggctatag
    40921 ttcctttcat aatagtagta tttttgtttt ctatttttag agtggtaatg ataagtctaa
    40981 ttggcagttc aaactttcaa gatatatttt ataatttatt ttcaaatgtt tttaataaaa
    41041 tgggaacaaa ccttatgagt gctctgttgt ttattttttt aatgcacttt atgtggtttt
    41101 ttgggattca tggtagtaat gtattagata ccgtcgcaaa aaatttattt gaaaatagta
    41161 tggcaattaa cataaattta gttaataata atcaattacc aacagaaatt tttactaaga
    41221 ccttttttga tacaatggtt ttattgggag gatgtggctc tttactatgt ttagtaattg
    41281 caatttttct tagtgaaaaa cgtataaatg taagaaagtt agctaaaatt gcttcaatac
    41341 cagctctttt taatatcaat gagatgctag ttttcgggat accaattgtt tttaactata
    41401 ttatgtttgt accttttgta attactccta taatcttaac cgttacaagt tatgtggcta
    41461 tgtctacagg tattgtacca tgtacagttt cagctgtaga atggacctca cctatatttt
    41521 taagtggata tatgtctaca ggttcaataa gaggtagttt gttgcaagta tttaatttat
    41581 gtgttggtgt tatgatttat attccgttta taaaaatgtc gcaaaataga tatacatata
    41641 tgtttaaaaa taatatagaa aatttaacaa acttattaaa aaggtatgaa ttaacaggtg
    41701 aacagcctaa tcttctaaac cataatggta aaataggttc aatttcaaaa atgttggcat
    41761 cagacttgaa gtttgctatg aaaagaggag aaatagagct tttttatcaa ccacaagtca
    41821 attataatgg aaatgttgtg ggagcagagg gattacttag atggaaacat tcaataggag
    41881 ggttcattta tccaccacta gttataattt tggctaaaga agaagggttc ttagatgagc
    41941 ttggagagta tattattaat aaagcatgta ttgatttaaa gaagagtgaa aagctattga
    42001 gaaatcctgt taagttctct gtaaatatat ctccagacca acttgatgac cctaagttac
    42061 cagagcaaat aaaaaatatc attagtagta atgatataaa tcctaatatg ttaggtatag
    42121 aaataacaga gcaaattgca ttatcaggtt cacaaataat tattgaacgt atcaatgcga
    42181 tacataactt gggaataaag ttaattatgg atgattttgg gatggggcat agctctctta
    42241 tatatcttca aaataataac tttgatatag ttaaattgga tggttcatta gttaaagaga
    42301 tacttacaaa taaaagaagt agtgaaatta taatgactat tgtaaatctt tcaaaaaatt
    42361 tagattttga tatagttgtt gagtatgttg aaaatataga ccaaagggat aagttatatg
    42421 gtttaggatg tgaaatatat caaggatatt attatagcaa agcaatacct tttgaagagt
    42481 ttgtagaata tgcaaatcaa gaaagagaga tataatttta taagagcttg taaattctag
    42541 taatgcatag atttgtaata aaattaagtt taggagaaat atatgaatat aagtatagta
    42601 gtagtaggta aaattaaaga aaaatattta aaattaggta ttgatgagtt taagaaaagg
    42661 ctttctaagt attgtaagtt agaaattata gagttggatg atgaaaaagc tccagagaat
    42721 ttgagtgtta aggaaatgga aattataaaa gacaaagaag gaaagaaaat tttagggaag
    42781 ataaagcata atagttatgt tatagccttg gctattgatg gtaagggttt atcctctgaa
    42841 gagcttgcta agactatgag tgatttagca gttagaggta atagtagtct ctgttttata
    42901 ataggaggtt ctttagggct ttctgatgag gttttaggaa gggctgatta taggttatcc
    42961 ttttctaaga tgacttttcc tcatcagatg atgagattga tattgttaga acagatttat
    43021 agggcttata ggataaataa tggagaaccg tatcataaat aaatgatacg gtttttgtta
    43081 atttgacact taataatatt aaaataacaa aaaagtattg aaagtatatt gcatttaagg
    43141 tagaattaaa taaaatatta taacattata atgtttatat ggttgttaat aaaaatttgg
    43201 aggaaaatat atgataaaaa taatgttggc ctgttcagca ggaatgtcta caagtttatt
    43261 agttactaaa atggaaagtg cagctaaaga aaatggtata gagtcgcaaa tttgggcaat
    43321 accagaatca actatacaaa atgaaataga aaaatgtgat gtattattac taggaccaca
    43381 agttagatat gtattaccta aggctcaaga aattgctaag ccatataata taccagtaga
    43441 agttataaat atgatgcact atggaacagt taatggagaa gctgttttaa atagagcgat
    43501 agaacttaac aacactaagt aatataaatt ataatgtatt tttaatatat aaattgggag
    43561 aatcaaagga tggatgaaaa gactatagaa atatctttta atataatagg ttatgcaggt
    43621 gaaggtaaag gattagcttt tgaagcaata aaagaggcta agaatggaaa tatagaaaaa
    43681 gcaaaagaat tattaaaaga gtccagggaa gttataaata aagcacatag atatcaaaca
    43741 gaattaatac aaaacgaagc tagtggaaac aggactgaaa taagtgtcat acttatacat
    43801 gcacaggacc acttaatgaa tggcatgaat tttcaacagt tagctgagga aatcgttgat
    43861 ttacatttga aattacaaga taaataaaaa ttaaagataa taaattaaga gagttcttaa
    43921 aagatacatt atgtgtagca cactaattgt tagattattg attcaatatt tagtgagtaa
    43981 tacattagga ttattttgag gactcttttt atatttagtt taaagttaat aaatatttat
    44041 tattaataat ataagattat atgttataat attataacat ataatttaag aggtgaatca
    44101 tgttaagaaa tcaaccatta tatatacaag ttgtagatat attaaagcaa aaaatattaa
    44161 ataacgaata taaaattgat gaaaatattc caacagaaag ggagttagag caagagtttg
    44221 gagtcagtaa aattactatt agaaaagcta ttgagatatt agaatcagaa gggtatgtag
    44281 agaaaaaaag tggaaaaggg acaaaagtaa taagtaatag tctttttaat aagctatcaa
    44341 aagcagagtc tttttcaagt atacttttat ctaaaggtca taagttatct aaaaagacta
    44401 tttcaattga aaaaataata aatgatacaa attcacaagt atttagtata tttggagaaa
    44461 aatgtagcaa gattactaga atgtattact tagatagtaa gccatatata ttttttactc
    44521 attatattcc ttttgatgaa aatctaactg ttgaaagttt taataatgaa agttctttat
    44581 atatgtactt atataggaga aatataaata taactagatt tgaagatgaa ttttttatag
    44641 aagaatcaga actagaggta tcaaaggctc taaatctagc taaagacata ttattaggaa
    44701 gaaaaagaaa gacctatgat gagaatgata atatagttga gctatcattt gcaagatata
    44761 atacagaagt acaaaactat gtaattaaat acgaaatata ataagaatcc tgagtttatt
    44821 gaaatgataa tatgctcagg atttttgttt agttgtaaaa ataaaaaata ttataacatt
    44881 ataatgttta caactaaaca ttataatgtt ataatatttt taataaacaa gtgatattat
    44941 caattacata aaaattagtt tatgttatga aatcgttatt gcattataaa taaaggataa
    45001 tatcaataat acagggggat aactatggaa aaattcgcaa gttttttaga gcgcaagatt
    45061 atgcctgtag cagcgaagat aagtaatcag agacatatga gagctgtaag agcaggtata
    45121 atagcaacat taccactaac aatagtgggg tcatttttca caatattatt aaacatacct
    45181 attgatgctt atatggattt tatagctcca tttaaagcaa tattagatat accatttaga
    45241 tatactgtag gatttttatc tctatatgct acttttggga tagcatcatc acttgctaaa
    45301 agctacgatt tagatacaac aggtgttggt atgcttgcag ttatggcatt tttagtttct
    45361 acaatagttc caactcaagt agttgaacca gtaggtggag ttatagaagc tggtagatgg
    45421 atggatatgg caaaacttaa ttcgccatca ttatttggag ctatcgtaac atcattaatt
    45481 acagttgaaa tatatcgttt tatgaaggaa aagaatatta caattaagat gccagcagga
    45541 gttccagtag aggtttcaaa ttcatttact gcattattac cgacagcagt aattttaatg
    45601 ttattctggg taataagaca tattttagga tttgatataa gtgttgcact aagcgctatg
    45661 ttatcacctt taaaaggatt cttatcagga aatagtttag gtggtgggtt actgacagta
    45721 ttcctaataa ctttattctg ggtacttgga atacatggtc cagctataat ggggccagta
    45781 ataagaccta tatgggacat gtctatagca gaaaatataa ctgcattcac agaaggagta
    45841 tcagctcatg caatgccaaa catatttaca gaacaatttt tacagtggta tgtatggata
    45901 gggggagcag gggctactct tgcattagtt gtaatgttta tgttttctaa gtcacaatat
    45961 ctaaaaagtt taggtagatt aggattttta ccagggttat tcaacataaa tgaacctata
    46021 atatttggag caccaatagt tatgaatcca atactaggga taccatttat aattggacct
    46081 ataataatga caattctatc atatgttctt actattacag gggttatacc tatgatggta
    46141 gcaagattac catttgcaat gccagcacca atagcagcag taatgagtac taactggagt
    46201 atattagcag gtatcttagt attaatcaac tttgtgatat catttgctgt ttattatcca
    46261 ttcttcaaag tttttgaaaa acaacaatta caaagagaac aagaagaaat gagtgaaaat
    46321 ttataaaaat acatagatat agaggttttg tactatataa aatatagaaa aaacttataa
    46381 gttattaagg gtgaaggtag gaggaatgac ctttgccttt aattttatat attttaaata
    46441 atgtaatttg aggaggagaa agatggaaag aagattagga atatcagtat atccagagca
    46501 tagtaacatg gaagatgata aggcatatat aaaaatggct tcaaaatacg gatttaaaag
    46561 aatatttatg tgcatgcttt caattacaag acctaaggaa gaagttaaaa aacattttaa
    46621 agagattata acctatgcaa gagaattagg atttgaagta atccttgata tagctccaaa
    46681 catatttgat atattacaaa tatcttatga tgatttaaca ttttttagtg agttaaatgc
    46741 tagtggaata aggcttgact atggatatga tggaagtaaa gaagctatga tttctttcaa
    46801 tccatatgat ttaaaagtgg aactaaatat gagtaatgat gtagcatact tggataatat
    46861 aattacttac aagccaaatg taaataatat atatggttgt cacaattttt atcctcaaag
    46921 gggtacaggc ttgccatatg atttttttgt aagatgttca aatagattta aaaagtatgg
    46981 aataagaact gctgcttttg ttaattcaaa atatgcaaca atcggacctt ggaatataaa
    47041 tgatggactg tgtacgcttg aaatgcatag aaacttagat attgctacag caactaagca
    47101 cttattcgca acaggattga ttgacgatgt aattgtaggg aatgcatatg caagtgaaga
    47161 tgaattaaaa agaatgtctg aagtaaataa atatcaagtt gtatttaatg tagtaactga
    47221 ggaaaaaact tctgaagtag aaaagacaat aatggcaaat gagcaacact ttagaagagg
    47281 ggatataacc aaccaagtag caaggtctac agaagtaaga aaaaaatatg ctaaagaaaa
    47341 tttccctaag catgataata cgaccatctt taaaagagga gatgtagtga ttggtaatga
    47401 tgactttgga aagtataaag gggaattaca gttgatactt caagattgtg cagatgaaag
    47461 aaaaaataag gtaggtagaa taaaggaaga cgaaataatg cttttagact ttattgaacc
    47521 ttggagtaaa tttaaactta ttttagaggg agagtaaagt gaagatagac atattaataa
    47581 aaggtggaaa aacagtagat aaaagtataa ttgatatagc tatattagac gataaaataa
    47641 tagaagtaaa aaatcagata gatgattcta agtatcaggc aaatcaaatt ataaacctag
    47701 atggagaaaa atttattagt gctggttgga tagatataca tacgcattgc tacgaaagac
    47761 tagaccttta taaggattat ccagatgagg ttggaataaa aagcggtgtt actactgtag
    47821 tagatgcagg aacaacagga gctttagata taggtaaatt ttatgaagat actaaaaaat
    47881 ataaaacaaa tgtatatgct ttaataaata ttgctaagca gggaataaca tctcaagatg
    47941 aactttcaag tatgatgaat attgatgaat atgaacttaa aagagcagtt aaaaaataca
    48001 aagattttgt tgttggaatc aaagctagaa tgagtaaaag tgttgtaatt tcaaatgatg
    48061 ttgaaccttt aaaagtagct aaaagaataa aaaatgaact taatctacct atgatggttc
    48121 actttgggag ttctcctcca acgatagagg atatatttga ctatatggaa aaaggagata
    48181 tattaaccca tatatataat ggaaagccaa atggaatact tagaggcaat gaggttaaaa
    48241 aagaaatcat agaagctaga gaaagaggta taatccttga tgtaggtcat ggaaccgaaa
    48301 gttttagtat ggatattgca atgaaatcta aggatgcagg aatttttcca gacactataa
    48361 gtacagatat atatattaaa aatagaataa atggtccagt gtataatcta tctacaacaa
    48421 tggaaaagtt tatatatatg ggctattcac ttgaagatat aatagataaa gttactaaaa
    48481 atgcagcaga tgcaataagt ttaaaaaata aaggtttaat aaaagaagga tatgatgctg
    48541 atttaactat atttgatgtg gttaatgaag aaaaagaact tcaagattct ttgaataagt
    48601 cagtaatcac aagtacgtct ataaagccaa gagcagtagt agtcaatgga gaatatttaa
    48661 atataggtaa gtctatagga gagaatgagt agaagggaga aactattgta tgagtagtat
    48721 atttgaaaaa tactctgtaa gacgtgttat aaatgcttca ggcaaaatga ctatactagg
    48781 agtatcaaca gtagataagg aagttattga agcaataggt gaagggtgta aaaatttctt
    48841 tataatagaa gaacttataa ataaaacagg tgaatatata gcaaatttat tagggtgtga
    48901 aagtgcacta atagtttcta gtgcatctgc tgggattgca cagagtgtgg gagcaatgat
    48961 agcaaaagat gatatgagtc tagtttacaa tgtaaataat cctaagaaac aagttaaaag
    49021 ggaaataata attccaaagg ggcataatgt tgattatgga gttccagtag aagtaatgat
    49081 aagacttgga ggaggagaag ttgttgaggc cggctatgct aatatgtgta acaaggaaca
    49141 tataataaga gagataaatg aaaatacagc agcaattatg tatattaaat ctcatcattg
    49201 tgttcaaaag agtatgctaa gtgtattaga tgcagtacaa gttgctaaag aatataattt
    49261 acctttgata gtagatgcag cagcagaaga agatttaaat gtatattatg aattgggagc
    49321 agacctagtg atatacagtg gtgctaaggc tatagagggt cctagctcag ggttagtaat
    49381 aggtaagaaa aaatacatag ataatgtaaa gcttcaatca aagggtatag gaagagctat
    49441 gaaaatagga aaagaaaata ttgtaggtct aacatgtgca attgagagat atataagtaa
    49501 tgataaagta actttaaaag aaatggagga caagctaaat ccatttatag acaagataaa
    49561 tactataaag ggagtgagtg ctagtataac tagagattca gcaggtagag aaatacttcg
    49621 tggagaaatt gactttaatg aagatataat aaataagtca acacaacaaa ttataaatga
    49681 acttagaagt ggagagatag ctatatacac tagagactat aaggctaatg aaggcaagat
    49741 tgagattgat ataagaagtg taactggaag tgatttagat actatttata ataggataaa
    49801 aaatatagta gaaagttaga gtgtagagta agtgacatta taatgattta ataaattaaa
    49861 ttttaccaat ggaggggata aatatgaatt gtataccaca atattataaa aatagagttt
    49921 gtttaaatgt tcttgcagga agcataaaaa atgctaaaga agtatatgat gcttgtgatg
    49981 ggtatgtatt gataggagta ctatcaaaga attacagtac tgtagaagca gctattgaag
    50041 atatgaaaaa atactcaaag gaaatagata atgcaatatc tgtggggctt ggagctggag
    50101 acccaaatca gtcaaatatg gtatctcaga tttctaaaga acttcagcca caacatataa
    50161 atcaagtgtt tacaggtgtg gctacgtcta gagcattgct tggtcaaaat gatagtataa
    50221 taaatggatt agtttctcca actggtaaac caggatttgt aaaaatatcc acaggtccat
    50281 taagttcaaa agaaaaagat ggtatagttc ctatagaaac agcaatagct atgatgaaag
    50341 atatgggagg aagctcaatt aagttttttc caatgggagg gcttaaaaca aaggatgaat
    50401 atatagaagt tgcaaaagct tgtgcaaagc ataattttta tttagaacca acagggggta
    50461 tagacttaga taacttcaaa gagatagttc aaattgcact tgatgctggt gtagaaaaag
    50521 ttatacctca tgtttataca tcaataattg ataaagaaac aggtgaaacg aaagtggaag
    50581 atataggagt actttataaa ataatgaaag aattattggg atagatttat gaatatatta
    50641 gcttttggtg aaataatgat gagactttca gtacctgact acaagttctt aacacaaacc
    50701 aatgaactaa attatataat tacaggcaca ggattaaata tattatctgg acttaaaaac
    50761 tttggataca atacctatat gttgacaaag ttgcctaata ataatgtagg taaagcatca
    50821 agtgcaaata taagaaagct tggagtaaaa gatgacttta tcacatatgg agggaatcat
    50881 ataggagtgt attttcttga aaatgggtat ggagaaagac cttctgaagt gacttatttg
    50941 aataggttaa atagttcatt ttgtgagagt aaattacaag attatgatat tgataaatgt
    51001 ttagaagaaa tggatatagt ccttatttgt ggtattgcta tgcaacttac agattcaatt
    51061 aaagagattg ctttagaact agctagaaaa tcttatgaaa aggggataaa agtattcttt
    51121 gattttaatt ttagacaatc attaaatcca gaaaggactt atgatgattt agtaaatgat
    51181 tataagaaaa tactaccata ttgtcatggt ctatttgcta gttatagaga tataaataag
    51241 atacttaata tagatagtct aaatataaaa tctagttcag aattgattaa aaaagagtat
    51301 gatataggta ttgtagctgg aactatgaga agatttgatg atatgaataa tagatttata
    51361 aagggatata tatcaaatga tgatggttat tttgaaagta gggagtataa gttagaaata
    51421 tatgacaggg ttggtgccgg agatggatat gtagcaggaa ttatatattc atatttggaa
    51481 aatatgaaga gtgaagatat agttgagttt ggggttgcta gttcggtgtt agctcataca
    51541 acttatgggg ataattcttt agtaagcaca ggtcatgtac ttagacttat gaacaatgaa
    51601 aaaatagata tattgagata atacatttgt atataataaa atttttatct tttttagaca
    51661 cacttttgac ttgtttatat ttgttaaaca gagttatttt taatgattat aaatattaaa
    51721 ttaaaagtta aataataaaa tagtagaaaa tagcattaac tatagaaaaa atggttaatg
    51781 ctatttttat cgttaatatt gattaatttt atattataga aatgaacttt tctaatgctg
    51841 gatttgtatt ttttttattc catacaacta caacttcaat tctattttct acttctttta
    51901 taggtactat tgcaatatca cgttctatca ttgtatctgt aacacaaaat gaaggtaaaa
    51961 tggatattcc catgttagaa gataccaatg attcaatagt attaagatta tttgaacgtt
    52021 gtaccacatt aggtacaaac cctgcttcac gatatctatt ctgtatcatt gacttttgtc
    52081 catactcatc ccctgtttct tttacaaaaa taaatttatc atgttgtaat tcaattaaat
    52141 cgatagaatc ctcattgtaa agtggatgac caggaggtaa aagagctacc aaagaatatc
    52201 tttctacagt tttatattca aaatctttta atggttgatt ttctggatta ataacaaaag
    52261 cgaggtccat agaattattt tttactaagt ttaaaagttc ggttatttca ttagtacaaa
    52321 tatccataga tatattaggg tatgtattag aaaattcttt taaatattgt tgaagtttgt
    52381 ttttttcata tcctattaag gttccaatgc tcaaagttcc aaaaaaacca tgtgcaaatt
    52441 gttgtgtttt tgcaatagca tcattgatat tttttattat agtacgtgcc tcagataaaa
    52501 aaacttttcc agctggtgtt agttcaacat gacgtttact tcttgtaaat agagttacat
    52561 ttatttgttc ttctaaagct tttatttgct gtgtaacagc agtttgagaa atataaaatt
    52621 tttctgctgt tttggtaaaa cttaaatttt ctgctacaga tataaaatat tctaattgtt
    52681 tagtattcaa ataatcaccc ctatttaaac ttgattgttt gtaaacctta tttatctata
    52741 attaaagctt attatagtat aagaaaattt acttttaaag aatattagaa aatatttgta
    52801 taaaaatata tgttttaagt atatctttac attagatttt ataaatttat taataagaat
    52861 aacttatata ttccaatgaa aacgatattg gatttaagta agtttgtctc ataaaatgaa
    52921 agtatatcaa ttgataatac tttaaataag aagggagaaa aaatggaaga taaattttat
    52981 gcaaaaggca acggaaataa cggatatatt aaaaatcttg aagtttgttc ctttaataac
    53041 ttagatggaa cttgtggaat gtttcaaatg gctctgtaca aaagagatga aaaatactat
    53101 ttatatggat gctgttttgg aggaaataaa aaaaatggag taatgattag cgatattaca
    53161 gacccttata atccacaatt tataaaacat tttcaaatgt tagaccctaa agagtatcct
    53221 acaacaacaa ctcccaaaat tcaaatagca gatgatttaa tgatagtagc aatgagttgt
    53281 ggaagtggac caggagcact tgttgaccaa gctaaattag caaatattaa gtgtgaagca
    53341 ggaattagaa tatatagttt aaaagaagac cctttaaatc ctaagttttt aggatattgg
    53401 gattgtggct taaagcatgt aatgggtgtt catagattca tgtacaatgg tggaagatat
    53461 gtacatttat caagtgattg tgttggcttt gaaggtctga tttatagggt catagatata
    53521 ataaatccta ctaatccagt ggaaataggt aaatggtgga gaccagacca atatgcagat
    53581 ggatatccaa atagaacttt tgatgcagga gcacctcatt gcccagaatt tatggataaa
    53641 ggatggcttc atggacctcc atttgtaaga gacggaaaag catattgtgg ttatggagga
    53701 gctggtttag ttgtattaga tgttgaagat ttaacaagac caagatgctt aggtgaattg
    53761 ccatttatgc ctgcattttc tagtagactt gcaggtgcaa gaactcatac agcattacca
    53821 ttgccaggaa gagatttagt cgttgttcaa aatgagggag aaagattcca gttctttaaa
    53881 ccagataaca ttacagatgt tcaagctatg aataatatac atatggttga tgttagtgac
    53941 ccaacaaaac caacattaat tgctcaattt ccatatcctg aagttccaaa agatttccct
    54001 tatcctaact ttaatgttgc aggattagga aaaccagggc catttggtcc acataatctt
    54061 catgaaccaa tggataataa accatggtta gagcaaagag gagatagagt atattgctgt
    54121 tatttccatg cagggctaag ggtttatgat gtatcagacc catattatat caaagagcta
    54181 gcatatttta taccaccaaa tccaaataaa acaccagaag aatcttattt cccaggattc
    54241 ccaggaccac gcttggcagt aacagaagat cttatcgttg atgatagagg ctacatcatc
    54301 atagatgctt tagatgatgg attctatata ttaaaaatga aagaagatta aataaatact
    54361 aaagagggag agttgaaaat gaaaatacta ggtatttctt ttggaacaaa aaatggaaat
    54421 aatgacacaa tgtgtaaagt tgcacttaaa ggtgccaaag aagcaggggc tgaagtagaa
    54481 tttattcaaa tgtcatctat taacattaaa cattgtactg gatgttgtgc gtgtgttaaa
    54541 actttattat ctgggaaagg tagtatgtgt gtattaaaag atgattttga atggttgctt
    54601 gacaaaatga aagatgcaga tggtatagta gtttcagacc ctatttttga agaaggggca
    54661 tcaggattat tccatactat aatggaccgt tttggaccca gagcagatag aggaaataac
    54721 attataggaa caaaagttgc agaagctata ggaggaaaaa ttccagattc aagaatgctt
    54781 aatgataaag taatatcatt tatgggaata ggaggttctg actggggtac aagagttcaa
    54841 tgtgaacatg ctatgctagc tctcatacca atgtggaaag ttatagataa tgcttggttc
    54901 ccatgggcaa aggaattagt aatggatgat acaaggcttg ttcaagttca tcaaattggt
    54961 ttaaatatag ttgaagcagc aaaagatttt gaaaaagctt catatcaagg agaagaaggt
    55021 gtatgtccac attgccacaa tagattattc taccttgagc caggtactaa aaaggcaatt
    55081 tgtgctctat gtggtatagt tggagagtta gatactataa gtggaaaaac tatatttagt
    55141 tttccagaag agcaattagg tcatgctcat gatactttac caggtaagtt tatacatggt
    55201 gatgatatta agaaaatgga aggtcattat gcagaagttc gtaaaacaca agaatttaaa
    55261 gaaagaaaaa catcttataa ttttatagag acattaacta aagaaaaaca agctggagtt
    55321 tcattaggat aataattaaa atactaagac tcttttttaa gggatgttag atgttatagg
    55381 aggcttattg tatgaatgaa aagccaagtt ttaaacaaat tgaagttatt ttagcagtta
    55441 tttttttggt acttacatat tacaaggtta gattaactcc agcatttatt gttatattta
    55501 taccactata tttaagttat tttgcagtat ctatgattgt aaatcaaata gcaattaagc
    55561 tattggagat aaggcaagct agggaagcta ctaattacaa tataggaaat ggatagaaag
    55621 aaaaaagaaa tattaacact gtctatgggg attgctttac tacctccttt atgggcagta
    55681 ttagcacctt atataggaat aaaaactgga gctgtagcac taatctgtgc aggattatat
    55741 gtaactaatg gtaataaaca agaagatgga ttaaaaataa tgtttggttt ttggtgtgga
    55801 gatttatggg cagtgttagc cattttaatt atgggatata tgaatttcaa tcaaaaccta
    55861 gaattatttt taacacttag tatattaggt ttttttgcag ttgtaattgc atccttattt
    55921 gaaaaaataa ttttcctacc atcatggctt tgtggttggg ctataggtct aactattatg
    55981 acaggtgaaa atattattaa tttacaagat atttgtattc aaattgctgt agctatggca
    56041 gtaggagtat ggtacgtggg tgcaggtgta gatttgttta caatatatat tttgagtaaa
    56101 gataggaaaa agggaaattg aaagcatgtg aatatacttt gattatagga ggatggctat
    56161 gggttttgaa tttaaaataa tgagaagttt aatatatgta ggacttgcca aggaagaata
    56221 tagacctaag ctaatggact ggttatatcg tcaccatatt ccagatagta ttagcacttt
    56281 tggaccatat tgtactaaat atgcctttta tcaagcatat cctacaccaa atgaaggtga
    56341 gcgttttggt gcacgtaaga tgcaactaac agaacattat tggcttgtag atgaacatat
    56401 gcctgagatg gcaaatagaa ttatgacaga atatatgcct atggatgttc tacgttggca
    56461 agggtgtata ccagatgtag aaaataaaag ggttcatgaa aatgcagaaa gtggagatgc
    56521 aggacgtgca gtaggtggag ataatggatg tccaccattt atatttgcct ttgttccaat
    56581 aaactgggaa gaagacttta gaggaaaagg acgtactgta caagatggac caaactatcg
    56641 ttggcaattt atgattaagt atccagatgg tatctctaaa gaagaaggag aaaaatggtt
    56701 ctatgatgag gtagtgccat actttacaaa ctgttgctat gttaatcgtt ttgtcagtag
    56761 taaaataatg attaattatg gagcaactgc ttttgaccgt gtgtcagaac tatggtttga
    56821 aggggaagaa gaatggtata aagctgtggt tgaagagaca aagtcgttta ttaaaaaacc
    56881 agaatgggca caagaagagg agttcccata tttaaaacca caattcaata tcgcatcagt
    56941 attcttaggt gatatagcaa ctatggatgc atactcacag tatcgtggat atataccaat
    57001 gagataaaaa ttatagtttg tgataagtag agaaggcatt tactcttact ggtagaagaa
    57061 gacttattgt atccttaaaa gtaggtgaat atatagtttt atatttaaat ttatataaaa
    57121 ctaagactaa taaatatgaa acaatagaat tagaataatt tgataatatt tgttaaaaga
    57181 gcatttagaa tgtgtttaaa gattaagata ttccttttat ggtagcagat agttgaaata
    57241 ataaaactgt tatcatgaaa ggaatatttt tatgtatagg aaatttttaa aaataaaagt
    57301 taaatatgtt taagaacatc ttaaagtgaa agaatcattg ttagaaatat gtagacaata
    57361 tagtatttat aacgtatcat attaacaagt ttacacttgg attatgaaat aaaaagagta
    57421 attctaaagg ttgtaaatac tatgctgact ttgatacttc aaagtttagg tgattttgta
    57481 attgttaata gaaatacgtt taatgagcat aaagaattta tctaataata ggagaatata
    57541 atgtatacaa ttggacaagt atctaaattt ttaggaatat ctagagatac actaaaattt
    57601 tatgaagaca aaggattagt aaatcctaag aaaaatgatg aaaatggata tagaatatat
    57661 aatcaggttg atatctatga tattgcaaca ataaactttt atagagaaat tgatatagaa
    57721 attaaaaaga ttcaagaaat aagaaaaagt aaaagtataa acaatttaga actcttacta
    57781 gaagagaagg aacaaatcat actaaaagag atagaatata aaaagcttct cttaaaaaag
    57841 gttcagttag tcaaagaaga ttgtgacaaa atcaaaacat attttggaaa atacacaata
    57901 aaagaaatga agcctttgga agtaaaaggt aaaataaaac atgttactaa ctatgaggaa
    57961 tatgaagcct taaaagagaa tacaaacaac ttaaaaaaag cagttacatt aactgattta
    58021 agaagagtaa taaattttaa tgaagaaggt ataatagaag aaaaatttat aattgtaagg
    58081 gaagtagatg actacaacaa aatcatagaa ggtgaagttc tatctcatcc caattgtatt
    58141 tatactatta ttgaagatgg cagatggtca actggaggag aaaatattga taataaagtg
    58201 gaagacagat taagagaaat agcaatagaa aatgaatgtg aattacttgg aatagtctat
    58261 gttaatatat tgcttactac ttatgaagaa gggattgagc gtatattttt agagatttat
    58321 gcacctataa aataatataa aagcattgac ctgggactta ctcataggtt taaactcatg
    58381 atataagcag ttataactta tatagcaatt aaatattaat ttgaaatgag gattacttat
    58441 gagaacagag aaatcattaa aaaggcctat agccagtttt attattctaa caaatataat
    58501 ttttttacca ctttttttat tggtagtagt gactataatg ttaaaacttc caacagtgat
    58561 atctgacatt gcgttgtgta tatcagcgtg gtcatccact tttgcattta tgatattatt
    58621 taaaaaaatt tatccaggta agaaattttt agtacatgta aaagatagat ttagaaataa
    58681 acttaaattt tcaacagtat ctataataat tagtatacaa gtcctaatag ctgtagtagt
    58741 tatatttata ttagcatcta aaaatcatgg aataatgcct aactttacag tttcatcatg
    58801 gggagcattt atatatttat ttttaaaaaa tttatttgca ggatcattag gtgaggaatt
    58861 aggttggaga ggatttgttc aaaatcatct tcagaaaaga cattctcctt taaaagcatc
    58921 cataacagta ggtttttggt gggggatgtg gcatttacca atatggttta caacaggatt
    58981 caaaggactt gatttaataa aatatattat attttttatg attgcaatta tatcaatcag
    59041 tattataatg acaacttttt ataatctaaa taaaactttg ataataccta ttgcaataca
    59101 tcaactattt aattttttaa tagggataat taatgaagat ttaatagagc ttataaatta
    59161 ctatgcaatg ttatatttaa tatttgcaat tgcattaata gttataaatc caggaaatgt
    59221 attatataaa aatattagag agtaatatat atctttatgg taagatataa agatattatt
    59281 agaagaacat ccaaggagga ataaacatgg ttttactgat tgttgatgca caaaagttaa
    59341 taacaaacga gaggttatat aaatttaatg aatttgtagt aaatgtagaa aatttaattg
    59401 atacagctag aaaaaataat atagaagtaa tatatgtacg tcatgatgat ggagttgaaa
    59461 atgaattgac taaaggtaaa aatggctttg aaatatacga aaaatttaaa ccatgtaatg
    59521 gagaaaaaat atttgataaa aaagtaaata gtgcttttaa agaaactggt ctattagaat
    59581 acctaacaaa taatggtgaa aaagatatta ttattgctgg tctccaaaca gattattgta
    59641 ttgatgccac tataaaatgt ggatttgaac atggatttaa tataatagtt ccagcttatt
    59701 caaatacaac tgttgacaat ttttttatgt cagcagaaca atcatataaa tactacaatg
    59761 aatttatatg gaatgaaaga tatgcgaagt gtatttcatt agaggaaaca cttagaaaaa
    59821 tgaaataaaa atagagtatg aattgtatat gtacataagt tgatttcata aaaaaggatg
    59881 ttaaattcca agttaaaaat tagattaaat atattatatt atagataagg cacatcaatt
    59941 ttattttaaa ttaatgtgct ttttaattgt agtaatttat ttataatcaa tatgattttt
    60001 attatttaaa tcaatttttc ttgagtttct ttgtctaaag aaactttttt tatttataaa
    60061 atctacagtt atattatagt atctatttta gatgagttta atttttgtat caatctaaaa
    60121 caaaagttat acttaaatga aattgaaaac gataaaattc tcaaataaaa taagtattgt
    60181 ggaggtgatg aaagaatcca atgataatat gaagactaat cttaataaga aagtaggggt
    60241 taatggtagt ggacaaagaa attgtatcaa ttctccagat tttattaaat agcacattga
    60301 ttacaactaa tggattacaa gaggagtcta attcatcaaa gagacaaatt acttatagaa
    60361 taaataaaat caatgacatg cttaaagtaa aaaatgtacc acttatatgt ttaagagccg
    60421 acaaagatat tatcgttaga aaagaaacaa aaaaagcaat taaagaaatt ttagaaaaaa
    60481 actattctaa gaatacttat tattttagta agacagaaag acttttatat atgtatttaa
    60541 tgctttttat taacatggag gatatttcaa ttaatcattt tataaattct ataaaagtaa
    60601 gtaggtctac tgtaaatctt gatttcaagg atttgatacc agagcttgaa aagaagaaca
    60661 ttaaagttaa gaacaatcgt ataaatggat attacatagt tggcaatgag atggaaatta
    60721 ggagagtact tattaaaaac attatagaaa ctttgtctaa tgagaaaagt tcaagagtat
    60781 ttgatatttt tattaaagaa tataatttag atagttttga agaggctaaa aaaattatat
    60841 taaagctaat aaaaaaatat gaaattactt ttgttgaaga tagattaata gaatttatat
    60901 atatatttat ctttctaaaa gcaaggatgt attcaactag aatagttact caggaaaatg
    60961 tagatattcc taatatttca gttatgagtt caatgaagga gtataaattt gctaaggaat
    61021 tattagaagt atatgggagt gaagataaga taaagcctca caatataatg tatattagtg
    61081 cttggatttt aggtatatca gtaggaaatt atgaagaaga tacagaagat aaggatgtaa
    61141 tttcaaaaat tgtaaataaa atgatgacaa agtttggata tttaagcgga gtttactata
    61201 taagccaaga aaaaatattt aaacaattat attctcattt tagacctgcc tactatagac
    61261 ttttatttaa attacctatc tataatccga tttgtgaaaa agtaaaagaa gaatataaat
    61321 tagtttatag aattgtaagt aatgcaatga aagagttttg cggattattt ggagaagaaa
    61381 ttcaggaaga agagttagca tatcttacaa tgcactttgc aacacttttt tcaaataaaa
    61441 aagaatttga tggttttaga aaaagaatag cattaatagt ttgttcaaat ggcgttggta
    61501 gttcagctat tttacatgca gaacttacaa acttatttcc tgacttacac tttataccaa
    61561 tgtcggaatc agttggatta gaaaatataa gtgaacctgt agatattgta tttactacta
    61621 attataacgc agagggttta aaaacagatg taccagtaat taaagtaagc cctgttatga
    61681 ccacaaaaga aaaatataag attacacgtg aagtgtatat tcaattaggt gatatttttt
    61741 taagacaacc taaaatagat gaggttatgg atataataaa aaaatatgca aatgtaacct
    61801 ctgaaagtat tctttcaagt gagctattag cttattttac acagatagag aattttacat
    61861 ctaaggaagg agaggggcct atgctaagtg agattactaa tgaaacacta gtaaaaataa
    61921 aggtaaaagc taagaattgg gaggaagcta ttagacagtc agcttctgta ttagttgaaa
    61981 ataacaaagt aactgaaggg tatgtagatg ctatgattaa ctcagcaaaa gcatctggac
    62041 catacatagt aattacaaaa catgttgcac ttccacatgc tagaccagaa gctgggtcaa
    62101 aagagattgc aataggcatt gctactttag atagtcctat tgagtttggg aataacgaaa
    62161 acgaccctgt aaaatatgta ttttgtctaa gtgcaataga caataatagc catttacgtg
    62221 ctatgtctga gctagttgaa ctattggaag aagatgagtt ttttaaggtt ttagatagtg
    62281 ctaaagaagc taaagaaatt attgatttta taaaaacaca cgaattataa aggaggattt
    62341 aattatgtat aaaattttaa tcgcatgtag agcaggagtt ggctcaagtt taatgttaaa
    62401 aataaagact caacaaatta ttaaggaaaa taactttcca attgaagtag agcatggttc
    62461 attggattca ctaaatggat tcaatggaga tgctgtagaa acattaattg atgttgctga
    62521 agaattaaaa aataaaaatt tgaagtttga tataattggg attcacaata ttgttgaccg
    62581 taatgaaata aagacagcac tagaaaaatt ccttgcttca aaagagggat aaatttaaga
    62641 tgataaggag gaatatttat ggagtttatt attagtttgc tgagtacacc tgctgttttg
    62701 cttggcgtag ttgcatgtat aggattgatt cttcaaaaga aatcagctgt tgaagtattt
    62761 acaggctctt caaaaacact tatcggtttt ttgatatttg gtattggtgc ctctgctatg
    62821 acaggagcac ttcaaaattt caataaactg ttccaacatg gctttggaat tactggtgtt
    62881 atagcttcac cagaagcagc tacagcactt gcacaaggta catatggttt tgcagtttca
    62941 tgtacattaa ttcttggatt catattaaat ttagtatttg cacgtattac aaagtttaag
    63001 aatattttct ttacaacagg tcatagtttg ttcttttctt gtgtattagt tttaattatg
    63061 aaagcacatg gatttgataa tacaatcact attttaatag gtggaactat cttagggttt
    63121 atgtctgctg cactacctca attctgtcaa ccatttatgc gtgaattaac aggtggcgac
    63181 gaacaagcta ttggtcactt taacatgatt ggttatggtt tatcaggtta tattgggcgt
    63241 ttattttcaa aacataaaga tgatacaaca gagactatag aatttcctaa gtggttatca
    63301 atattcagag acttcttaat gggtctatcc atagttatgt taatcttatt ttatattgca
    63361 acattaaaag ctggtcaagc atttactcaa gaaattgctg gttcaacaca ttggttggtt
    63421 tttccgctta tagaggcatt tacatttgtg gcaggtatgt caatattgat gtcaggtgtt
    63481 cgtatgttct tagctgaaat aactgcagca tttgtaatca tttcagaaaa gtatattcca
    63541 ggttcacgtc ctgcgctaga tgttccaaca gtatttccat atgcaccaaa tgcagttatt
    63601 attgggttta tatcagcata tgctgctggt ctattagcaa ttgctattat ggcatcattt
    63661 cctagtatat ttcctgtagt aattattcca gctgctcaca tatgtttctt ctcaggaggt
    63721 acagctgcaa tatttggcaa tacttcagga ggatggagag gggctattgc tgggtcattt
    63781 gttgtggggt tattacttgc atttttacca gttatactat atccagttta tggcactctt
    63841 ggtatagaag gagcaacttt ccctaatatt gattacaatg tagttggttc gatattacat
    63901 aatattttac aatttataaa acctatttaa actaaattat ataaaagtat agtcatagtt
    63961 actatgattt caaggaggat acatcttatg gaaaatacat tatctgaatt tttagataat
    64021 atatctaatg aattgtcaga atttattgcc tcaatagatg agacatctat aaataaagca
    64081 tgcgaattaa ttcttgaagc tgaaaaaaat cacggacgtg tacatgtgac tggtattggc
    64141 aaaccaggac atgtatcggg atatatttca tcattgcttt catctactgg aacgtcagca
    64201 tatatattgc atggtacaga agcagtacat gggtcaagtg gtcaagtagt tgaaggagat
    64261 gttgttattg caatatcaaa tagtggtgaa actcaagagt taaaagcaac tcttaagact
    64321 ctaaaagtaa atggagcaaa gataattggt gtaagtggta atgagagttc atttttaaaa
    64381 aatatttctg atatattcct gtttgcaggt gttaaacagg aaggagattg tcttaataaa
    64441 gctcctcgtg cttcaatttt agctgaaaca ataatattac aatcattaag tgttgtatta
    64501 caatatgcta aaggtcttaa tacacagcag tatttgaagt ggcatccagc aggaagtctt
    64561 ggaaagtcta ttagagaggg aatataattt tatgaaatat aaagggatta tatgtgcaat
    64621 gataacacca tttgatgaaa atcaaaatat taattcacaa gcaacatgtc aattaattga
    64681 ttatttgatt gaaaagggaa tttatggatt atttattttg ggcactaatg gtgaatgtca
    64741 tgtattaaca gatgatgaaa aagttgaatt tgcaaagatt gttattaatc atacaaataa
    64801 tcgtgtacct gtttttgttg gaacaggggg taattcaact cgtgaagttg tagatttatc
    64861 taagagaatg gaagcgattg gagcaagtgc attatctatt attacaccat actttgttac
    64921 accaacacaa caggaattaa tcttacatta caaaactatt gctaattcag ttaatttacc
    64981 tattataatg tataatatgc caagtaagac tggaattaat attgaaccag agtcagtacg
    65041 tgaattatca aagataaaga atattgttgg aattaaggat agtagtggta aacttgacaa
    65101 catgaaagct tatattgaag caacgaagaa tgagaatttt agtgtgtttt caggctcaga
    65161 ttcacttatt ctggatagtc taaaagctgg tagtcaagga gctgttgcag caacaacaaa
    65221 cttcctcaca gagattggta tctctatcta tgaaaacttt atgaaagggg atttggaaga
    65281 agctcaaaaa gctcaaagta gtatagaaga acttcgacgt atactgaaac ttggaacaat
    65341 tccatctgtt atgaagaaaa ctattgtctt gaatggaatt aatgttggaa ctgcacgact
    65401 tcctgtaact gaaccaactg gggaagtact tgaagaaatt aaacgagtag ttgaaaatta
    65461 taggacaata cttaataaaa gtagtgagaa tagataatat aaatagggaa attacactgg
    65521 tatataatta atatatatat tataaaacat gactaaaatt aatgaataag taaagtggtt
    65581 tgatgagtat agaggtgata atatgaaaaa gatgaatata accaaaagaa tgtcagaatg
    65641 tggggctctt gcaattgtaa gagaagaaaa tcttaaccgt gcatgtgaaa ttgctgaagg
    65701 atgtataaaa ggtggtataa cagttataga aatgagttat accctaaata atgctggaga
    65761 aataattcaa gggcttaata aaaagtatgg tgaaacactt tgtgtgggag caggaacagt
    65821 tttggatagt gaaacagctc gtcatgctat attacatgga gctcaattta ttattgcacc
    65881 aaattacaat gaaggtgtag cgaagatttg caatagatat cagattcctt atgctccagg
    65941 atgtacatca ttgactgagg cagtagacgc actaagttta ggagcaactt ttataaaagc
    66001 tttcccaata tcagatttct atggacctaa actagtaaag gtatttaaga caccaatacc
    66061 tgatatgcca gttttagcaa gtggagggat taatattgaa aacctacata tatggttaga
    66121 aaatggagtt gatgtatgtg gatttggtgg tttattaact aaaggttctg ttgaagatat
    66181 tgctaaaaat gctaaaaaga ttcgtgaaat cattaaaaat ataagaaaaa tttaacatag
    66241 ggctgtatca tagtatctta taattactat gatacagcct ttttaatatt attcaaatta
    66301 aaaacagttt acatttctat tatattacat ataatttcag taattttatc ttaatttttc
    66361 tatctggaaa aaaatgattt aaaacttaag ttatgatata atttcattaa aagaaggcga
    66421 tttttagtaa atatgagaaa ataatgtgtt tttaggggaa aagttaaata cagatttaag
    66481 tgaacaagaa tcgataatta agatattaaa tggtgaatag ggcatcaaaa aaattactaa
    66541 attaaaagtt ttagagggag aatcttagca agtgtgtgtg aaaattcaat ttcaaacaaa
    66601 aaataattgt aaatgaaatc aatttataca cgttgaagat tcacttttat cattatatta
    66661 aaaagcagag tgaattaaaa gtgagtctaa agataggagg ataattaaaa caatgaaaga
    66721 agacattagg ttacatcgaa tgtgtaaaaa agaacaagca acaaagaatg aagttatatc
    66781 agaggatatt ctaaaatcta tgagtattgg ggtcagtaaa cattttttag atgattcatt
    66841 tacaataata tggggaaata catcattcta tcaattatta ggatatacag aagaagaatt
    66901 tttagttaaa ttttctagtt taaaagatta ttatgaacag aatctttatc attttgaatc
    66961 tatgaaaaat cattttttag aggcttatca tcatggcgaa aagagtatag aatatcaaat
    67021 ttgcatttca gcaaataatg gagaccctgt ttgggtaata atgactggta tattaattga
    67081 atcacaagat aatgagaaat caatggtcta tatattgtat tcaaaaatta atgagttaaa
    67141 aataaagcaa gaagaactat ctgatatgga aaaagagagt acagagaatt ttaaatggat
    67201 gatgtcagag tatgctggga atgtttatat tagcgatatg gatacatatg aattactata
    67261 cttaaataag cactcatgca atactttaca ggcatcagca aatgaactta ttggaagaaa
    67321 atgttatgag gtaattcaag gtagaacttc gccatgtccg ttttgtacaa attcgtattt
    67381 aaaaaaagat gacatatatg aatgggaatt ttataataaa aaactgaaac gaacatttat
    67441 aattaaagac cgtatgttga attggcaagg gcgaagagtt cgtattgaat tatcttatga
    67501 tatgtatagt gctgaatata aattagcaaa aaaagatcag gaaagagaat caattttgaa
    67561 aactattcca gcaggaatgg ttagattaga tgcaagagat tatcttacta tactttggtg
    67621 taatgatata tttttgaata tgattgaata tacaaaggaa cagtttgcag aggaacttaa
    67681 taatcaatgt ggatatttat tttctgaaga ttataaacgt agtaagaaac tggcacaaga
    67741 tttgaaggag tctggggata atgttgtatt tgaagcaaaa atatatacac gttcaaggga
    67801 agaacgtata tggactgtta cattgtgtta tatcagtggg gaagacagtt gggatggaat
    67861 accatctttt tatagtatag gtattgacat aacaaaggaa agaaaacaga ttgagaaatt
    67921 acagcatata tcagagaaag atgcattaac aggaatttat aatcgtgctg agactgaaag
    67981 gcagattaag aaatattttg aaaaaaattt aaatgttatg ggtgcactgt ttatgattga
    68041 tacagataat tttaaacaga ttaatgatac agaagggcat atgattggag atatagtact
    68101 tactgagatg gcatcaggta tgaaaaaaat aatgcgtgac agtgatgttg ttggaagaat
    68161 aggtggagat gagtttacaa tttttatgaa gaatatttcc tcagttaaag atgcagaaaa
    68221 gaaagcagaa gaactattgt atatgttccg tcatctattt cagaaagaaa aaagttttct
    68281 aaaagtaact tgtagcatag gaatagctat atatccaaag gatggcacta cattcaagga
    68341 aatatatgca agagctgata aagccctgta tcaagcaaaa aatatgggaa aaaataatta
    68401 tgtaatctac aaccaagatt attttaaaga attagaagaa tttgattgtt cttctcttgg
    68461 tacagttatt gattcagaaa aacaatacgc tgaatatcct gataatctta cacgttatat
    68521 attccgtatg ctttatcaga ctgatgatat agaccaggcg attaatatga tacttgaggt
    68581 tgttgggaaa cagtttgatg taagtcgtgt ttatattttt gaaaatacag aagatggtag
    68641 atatacttct aatacttatg aatggtgtaa tgaaggaatc tcttctcaaa tggcttattt
    68701 gcaaaacagc aattatcaag actatgagga ttatgaaaag ctttttgagg atgaattagt
    68761 tttttattgt cgagatatcc atacattaag tcctaaacta gaagaattgt tttctagtca
    68821 aggtatccac tctacacttc aatgtgcata taaagaagat agagttttca gtggatttgt
    68881 tggatttgat gaatgcacag gactacgtct atggtcacaa gaggaaataa gtacattatc
    68941 attgatttct caaattatat caatgttttt acaacgtaaa aaaaataata aacttaatca
    69001 acagattcaa caataccaga atattttaga taatattgac cagtgtattt gtgtgattaa
    69061 taaagaaaat aattttttat tatatgtaaa taaaaaattt aaaaatacat attctaaatt
    69121 ccaaattgga caaccttatt atcttgattc tatagatatt cataagatat ccattatatg
    69181 gaataaaaaa gaagcatacc tatgtactag cacgatttaa tggtaaagta aagtttatat
    69241 tggacttaaa ttaaatttcg ttttaaataa gatattattt tagttgctta ctgaaaagac
    69301 tagaatctat attaatgaat tatactaatt ttataccact attaaaatat tgtttgctaa
    69361 aaataaaaaa tagccttaaa acagttgatt ataaaaaatt aactgcttta aggctatttt
    69421 tatctttgtg acgaaataga cacttgccat ttagaatgtg acaatatagt agttgtttag
    69481 aaaaggcaca aagcattata ctttaactaa agtaaaatgt attttgcttt tagaataaaa
    69541 acaattagga gggagctata attgaaaaag ataatatatg tattatttac aattgaacta
    69601 ttaatagtat ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta
    69661 tataacgaga caacaagtat atctgtaatg tttaaagatt ataaaaagtt aaataaaaat
    69721 tatagtaagt ggattaaaga tattgcagat gaaaataatg tgactatatc aaagtatgtt
    69781 tttaataata atgaaaaatt atctatatat acgactgata caagtttgaa taacaatata
    69841 aagttaaaat caggaaaatt tcctaatact aattcaaatg aatttattag taatataaac
    69901 actaataata gtaatcaagt aggtaaattc tcaactatga gtaaagacat atcaatttta
    69961 attagggatt ttggtaaaat aagtgaagtt ggagaatcag gaatcttata tatttcaact
    70021 caaaatgaag atacaataaa taaaatatta agagaactaa atgtagacag aagtattttt
    70081 gtagcaagat tacatgatag atatgttaac tcaaatttat acttaaatcc ttcaataatt
    70141 agagatttaa tactaattat cctttgtttc ttggctacaa taatacatta ttcaattagt
    70201 gtgagtagag aggcttctat tttaagatta aatggatact ctaagctaga tataattgct
    70261 agagttataa aaaatatact tagaattatg attttatcat ctgtcacagc attattaata
    70321 tatataatct atattttaaa attaaatata ggtgttagag catgcttata ctttgctatg
    70381 ttttcgatta tatgtatttt gataaatatt ttgatatcta tattaatagt tggttttaat
    70441 agtagaagtt ctaaatatgt gctatctcta aatggaaaaa aatcttatgg ttttgtaaat
    70501 ataatgcatt tcacattgaa aattgtattt gtatcatttc ttatattatc cataaataac
    70561 tgtattttaa attataatat gctacaaact cagttaggaa atctatctga gtgggataaa
    70621 gcaaaaaatg tttataatct tactcttaaa gatacaggcg aacaatcttt aggaaaggaa
    70681 gaagtaatta aaaatgccaa gattaaaggt ttctataaaa atttggtaga agaaaaagat
    70741 gctttcttaa tagattcaac taattttgaa aaacttgaag atggaagtta catgtatgaa
    70801 ataaattcaa agggtaaaaa cccagaagta agtcaatatg gtaaaaatat aaaaataaat
    70861 aaaaactatc ttaaagttaa ccctatttac tcaaatggca aagaaatatt taatctaatc
    70921 gattatgacg ataagactat aaatttatta gttcctaaaa agttacaagt acatgaaaat
    70981 gagattaaaa aagagtttag agaattattt tattttgaaa aaatagaagt agaaaatatg
    71041 tacaataaag agttaaatag agcgttaaac aaaacaaaga agtcagattt aaatgttaat
    71101 ataatatatg tagataataa tcaaagctat tttacttata actcccttgt tatggatgac
    71161 aataggaatc tcattgatga cccaatagca atagttgaca tagataatat tgatgattct
    71221 ttttatcttt cttatatatc tagatgtgta tattttaatt ctaagaaatt agatgccctt
    71281 gcagatataa gtagcattat tgaagctcaa ggtgtagaag cgcatatcca aagtttacat
    71341 gctgtttata atgagtatgg tttagaaata aataaactag aaaaatattt gaatagtgaa
    71401 atttttacta taataataat agcaatatca aacttaatga ttacctacaa tatagtagct
    71461 agctactatg aaagaaataa atataagtta tatattaaaa aaatatttgg ttattcaact
    71521 acagctagaa gtatgttgtt aatagtctca ttaatattaa caaacattat tccaataggc
    71581 attatatcaa caagagtaga tttaagtaat aatattatct tatttggact tctaatatta
    71641 gttatagaag taattgtatc tattgtatta gacaagatta tctcaaatag ttcatttaat
    71701 aaaattataa aaggagaaca ttaaaaagat gatttcatta ataaatatat ctaaaaaatt
    71761 taaagataaa tctataatag acaaatttaa cctagaaatt tctaagggcg agtttgtagc
    71821 gataacagga aatagtggtt caggtaagac aactttactt aatataatgg gattattaga
    71881 aaaacctaat tctggtgata ttataataaa taatataaaa aatccaaata gtaaacagat
    71941 aagaatttta caaagggatt tttatggcta tctatttcaa aattatgctt taattgagaa
    72001 tgaaaatgtt gaaaataact tgaaaattgc tttaaagtat caaaaaaata tcaaccataa
    72061 taaaaaaata aattacgcac ttgagtctgt aggtcttaga aaatacaata aaaaaaagat
    72121 atacgaattg agtggaggag aacaacaaag ggtagcatta gcaagagtaa tattaaaaga
    72181 atgtgaagta atatttgcag atgaacctac aggtaattta gataaaaaaa atagagacat
    72241 agtatttgat atacttaaaa gtctaaataa gaatggtaaa actatcgtat ttgtaactca
    72301 tgatttagag ttatcggagc aggcagatag agttataaaa ttgtaataat atcaactata
    72361 ataaatataa gaaagagatt acagctaaat caagtttaat aataatacga atagaattta
    72421 aacattaagt aaattagtag tttgtaaata taagtataat atgatatcat aaaggctcaa
    72481 aagaaatttt atataataag aaatctaaaa ataacaaatg gaggacatat aaagatggaa
    72541 aatttaccaa attgcccgaa atgtaattca gaatatactt atgaagatgg aactctttta
    72601 gtttgtccag aatgctctca tgagtgggca ttaggattgg gagctgaaga agattcaaat
    72661 attatcaaag atgtaaatgg aaatgtatta aatgatggag attctgttac agtaattaga
    72721 gaccttaaag taaaaggaag ttcatcatct ataaagattg gaacaaaagt gaaaaatatc
    72781 cgtttaatcc atgactcatc agatggacat gatatagaat gtaagataga tggttttgga
    72841 gctatgaagc taaagtcttc tgttgttaaa aaagcgtaat attataaata agctgtagtg
    72901 tttattgttt ttcagtacag tataaaacca taagaatcta ttaagtaata aaagtttgtt
    72961 tatttatctt tatattttct tatggtttta ttaaatataa attttattga acaccaaaat
    73021 attctctcct ctacaaataa gccaatctta tttaaattaa tatactttgt actatttata
    73081 ctgaaattgt aaatttaaat gctatgttat ttaggtttgt atagttaatt tatatatttc
    73141 tgattttata aattgtattt attattatat cttgattcta aaatcattaa aaatttcaac
    73201 ttctataaag atttacaatt tatcaaatac attatgagta tataaacaat ttattattac
    73261 agtgctacat ttaaaataag tcttagtata aaggtattgt tacaagtata aagaaactta
    73321 tgtatgctat tggaataaat aataccttaa attataataa agaataagaa ctgttaaata
    73381 agggacttga ttatcattta gttatagata atataatatt aaaaataaag tttaagagga
    73441 gaaaaaatat gatagatata cataaaaata tatatgataa taagttattt gaggagctaa
    73501 agattgactg taagaagtgc tttggacttt gctgtgtagc tctttatttt tctgcttcag
    73561 atggatttcc tatcgataag gaatcaggaa aaccctgtat taatttacaa ccagatttta
    73621 aatgttctgt acataatagt cttatgaagc gaggttttaa agggtgtact gcatatgatt
    73681 gttttggttc aggtcaaaaa gtagcacaag taacatataa ggggatagac tggatgcaat
    73741 catcagaact aacaaatcaa atgtctgaag tgtttttaat tatgagacaa ttacatgaaa
    73801 tgttatggta tcttaaagaa gcatctgtat taaatatatc agatacaatc aaatcaaaaa
    73861 ttgatttaat aattgaagaa actgaaaaga taactaatat gagcccagaa cagataatta
    73921 atttggatat catatcacat agaataaagg ttaatttatt attatcacaa gcgagtgagt
    73981 ctgtgatggg aaaggttaaa tcatttatca agacttctac tcttaaaaac atgaaaaaat
    74041 taagtaaaaa tatagattta ataggagcag accttagaaa aataaatcta ataggagcag
    74101 atttaagagg tagatttcta atagcagcaa atttaagaaa tacagatttg agtggggcaa
    74161 atttaatagg agcagactta agagattgtg atattagagg tgctaatctt gaaaatagta
    74221 tatttttaac acaattacaa gtaaatactg caaaaggaga ttctagtacg aagttaccag
    74281 cttcactaat acgccctaaa tactgggaaa aataattaaa ttaatacata attttacaaa
    74341 acatgaactt ttttgacttt aattcatatt atacattaga gattctttac aagaatttct
    74401 taatataata tagggagaga tataaaaatg agttatcaaa attataaatg tgaaagagat
    74461 tatgtgtatg atgattataa atacaataag tgcaacaagt gctataatga ttatgaggaa
    74521 atgacacatg ttcatgaata ttcagaaagt gttaagttag cagaagaatg tgaggataga
    74581 cataaccacc gtgcagcagg ggttactggt gaagctatac caataaatgg aggtacaaat
    74641 catgttcata aaataaatga taatgtagat ttccttgacc atttccataa gatatgtgtt
    74701 acaacagggc cagcaattag aatacctgga actgataaac acattcactt aatatgtgga
    74761 gaaactactg ttaatgatgg tcattgtcat aaattccttt ttactactca aatagaagca
    74821 cctttagtat aagctgatgt gaattattgt agtgaatttg ctagattata gtataataat
    74881 atataataga taatatttaa acttaatgag gactatgata tttaaccttc gtagtccttt
    74941 ctaagttttg tataaaaaag tgttatgcaa ttaaaagtga tatagggagg aaatttttat
    75001 gaaatctgga ataattcttg aaatgaaaca aataaattca acagaatatg acgaaatgtt
    75061 gcaagtctgg gaatcatcag taagagcaac gcatgatttt ttaacagaaa aagatattga
    75121 atctttgata ccattagttg agattggact taaagaagta gaaaatatag tttgtataaa
    75181 agataatgat ataataaagg gctttatagg aatagataaa gataaaattg agatgctctt
    75241 tatagaagat aaatatagag gaaatggtat tggtaaaaaa ttaataaaat atgctataga
    75301 taaatacaat gtaaaatatg ttgatgtaaa tgagcagaat aaaaaagctg taggatttta
    75361 tattcactta ggatttaaag tgtttgatag gtctgaaata gatggacaag gtaacccatt
    75421 tccaatacta catatgaaat taatttaaag ttatacttct catttattcg caaaaatatt
    75481 tacttaaatt tttatacaaa taaaagtcgt gacagtctta ggacacgact tttttactac
    75541 actattaaat tgaaatttat ctgttctata ttatgattat tagatgtcgt ttttttattt
    75601 taactatgta cttaaatacc aagtataccc atacctatca agtacttcag cagcgccatc
    75661 attttgatgt ggtggtgggt gtggtaaaaa ttcttgtaca agtgtaccac cttcggcaag
    75721 ttttacagat atttcttcta tcgcttctcg agatggtaga acaacacaaa tcgccatgcc
    75781 agaaattaca acaggcttgt ctgtatccgc acccattata caaccttcgt attttgtgaa
    75841 agtaacactt gcgtgcataa taggattgtt tggattgctg tctaatcgcg caaatatagt
    75901 ttttcctcct ccgaaagcct cagcataaaa attaaatgct ttttcacaag taccattaaa
    75961 tattaaatat ggagttagca tgaccaatat atcctccttg atatagaata atttatagtt
    76021 gcacctgata atctaaatta tgatttagta aattgtatca tagggaaacc ttgaagtata
    76081 taatttttta ttattatatt atacaaattt attaatttaa aaaaatgtta tatgtacgga
    76141 aggtttacag gataaaatat aataaaaaga ttaaggtgac attataaaaa atgtatattg
    76201 acattgacgc tacgtaattg tatatacttt gtcttaaagg agatggtaaa tatgaatatt
    76261 atagaaattc gctcagataa aatatacaag aagataatgg atgcaccaat aaacaaaaaa
    76321 gaagatatat acagatatga attgatgaag ccttttgaat ttaagtggaa gtgtatgaat
    76381 gttccaatag ttgctagaca gaaaggtgga tatgatgtaa ttatagcaag tgaaatgtta
    76441 ggggttttat cgcctaagga tattgatgaa aaacaaaaaa agaatataaa tgtgttatct
    76501 gctgataaaa tttgggccac ttgtaaagaa accatagaaa actctataaa tgcttttata
    76561 aaagaagggt atgatttaaa tattaaggac tataaatatt caatattatt ggcgaatcca
    76621 aatagtcctt atacaatatt aagtgatgga tactggggtg atggtgggat tcctggatat
    76681 atatttctat cattggttcc taatgaatat actatcaata gattaccagt attaatagca
    76741 catgaatgta atcacaatat tagatttcag tttatagagt ggaataataa tataacatta
    76801 gaagaaatga tgataaatga aggtcttgca gaaaattttg caacatggat gtttggagag
    76861 gaaatgttag gaccttgggt cagtagaaca gatatcgaaa cattaaatac ttatataaag
    76921 ccaataataa aaagtgcttt aaaagaaact ggatttcaaa atataacatc ttatctttat
    76981 ggtgatgata tagctaaaat gcaaggatat tttccagtag ggttgcctta ttgtgcagga
    77041 tatgcttgtg gatattatat gattaagtat tatttagaaa agacaaataa atcaataatc
    77101 gaagcgactt tattgcctta tagtgagata atcgaagcag taaaagagtt ttgggaataa
    77161 attttataaa ttgcataatt tgaaataaat tatgcaatta ttttattttt aagaaaaaat
    77221 aaattgacaa taatttataa gaaatataca ataatatata taaagaaata tttcaatatt
    77281 taaaagtttt aagatttcat atctagagta cctaacatta catgttagtt tcttgtt
//
LOCUS       DAMJVI010000006        65324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.006, whole genome shotgun sequence.
ACCESSION   DAMJVI010000006 DAMJVI010000000
VERSION     DAMJVI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 65324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.006"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            81..1277
                     /locus_tag="PC545_000502"
     CDS             81..1277
                     /locus_tag="PC545_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDF2455294.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEEALKECIDLAPLHNPANIMGIDACKAILPDVPMVGVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            1370..1894
                     /locus_tag="PC545_000503"
     CDS             1370..1894
                     /locus_tag="PC545_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDF2455295.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            1919..2095
                     /gene="rpmF"
                     /locus_tag="PC545_000504"
     CDS             1919..2095
                     /gene="rpmF"
                     /locus_tag="PC545_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDF2455296.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            2302..2859
                     /gene="fapR"
                     /locus_tag="PC545_000505"
     CDS             2302..2859
                     /gene="fapR"
                     /locus_tag="PC545_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HDF2455297.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            2870..3892
                     /gene="plsX"
                     /locus_tag="PC545_000506"
     CDS             2870..3892
                     /gene="plsX"
                     /locus_tag="PC545_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDF2455298.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVALNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDINQFISKYNEEN
                     KNNEDE"
     gene            3918..4904
                     /locus_tag="PC545_000507"
     CDS             3918..4904
                     /locus_tag="PC545_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDF2455299.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVNEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            5013..5942
                     /gene="fabK"
                     /locus_tag="PC545_000508"
     CDS             5013..5942
                     /gene="fabK"
                     /locus_tag="PC545_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HDF2455300.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            5958..6908
                     /gene="fabD"
                     /locus_tag="PC545_000509"
     CDS             5958..6908
                     /gene="fabD"
                     /locus_tag="PC545_000509"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDF2455301.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAQVDLKYSACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQDTVPTGVGAMAAILGLDRSILENVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAMVKCKEAGAKRAVKLNVSGPFHSSMLREAGVKLASELEKV
                     NITKPKVDVVANVNADYYKNEGKDLLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            6908..7657
                     /gene="fabG"
                     /locus_tag="PC545_000510"
     CDS             6908..7657
                     /gene="fabG"
                     /locus_tag="PC545_000510"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDF2455302.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIESMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMSSYITGQVINVDGGMVMQ"
     gene            7711..7935
                     /gene="acpP"
                     /locus_tag="PC545_000511"
     CDS             7711..7935
                     /gene="acpP"
                     /locus_tag="PC545_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2455303.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            7964..9202
                     /gene="fabF"
                     /locus_tag="PC545_000512"
     CDS             7964..9202
                     /gene="fabF"
                     /locus_tag="PC545_000512"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDF2455304.1"
                     /translation="MNKRVVITGLGCVTPLGTGKEEFWSNIKSGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAIVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            9428..11146
                     /locus_tag="PC545_000513"
     CDS             9428..11146
                     /locus_tag="PC545_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2455305.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIILFIKHDQNGLKEREIINKG
                     KYMSESLVADKEYDEAKKVVKTSFESIPEKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDVKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASIFLKIGGYETGEKLIKDSFKIDIDEGEKNGMVRLYAIINLAEIYVEEGEY
                     DKAIEISSRIKDYKKFVNTDNYNDFDIMASIMQSNAYVGKNNIQKAKTFLEKADYLIK
                     SDRTVYILDKDMYYYMAMGNLEYKSENYNSAIDNYKKSLEISTKRKLTQEKIKNLNKI
                     LNIYEKQGNIESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESKLFEKTKKEIK
                     YYKILFTVILIAILGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYREKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDTVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGDEVADIIDSIRVAIANKKWNDDVTVTISAGVAHVSDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            11177..13177
                     /locus_tag="PC545_000514"
     CDS             11177..13177
                     /locus_tag="PC545_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2455306.1"
                     /translation="MKQSIAIVTDKITKLSSFLEENIRLVLGNYIDINHYYLEVLKEN
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKGKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGIINTFNKEALKILGINENPQGKNIEDIISEDLNILLEDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMVKCVELAKKISKSELSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLLKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYIRVECANNYEDGFGYTRKEFEEILRILYERRESSSGIGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            13528..13773
                     /locus_tag="PC545_000515"
     CDS             13528..13773
                     /locus_tag="PC545_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2455307.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGLKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            13808..14533
                     /locus_tag="PC545_000516"
     CDS             13808..14533
                     /locus_tag="PC545_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDF2455308.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDINPQLYGEETREETTFIYPEVDEFDLIAISI
                     ALELKKPILGVCRGLQILNVSLGGTLYQDNNLIEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDENFVVGVQWHPE
                     MMVDYCDKMEKLFKYFINICSNL"
     gene            14555..15868
                     /locus_tag="PC545_000517"
     CDS             14555..15868
                     /locus_tag="PC545_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2455309.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKSFGVASIFVY
                     IFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVVMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTSSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARICFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYDENLKKIV"
     gene            16074..16613
                     /locus_tag="PC545_000518"
     CDS             16074..16613
                     /locus_tag="PC545_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455310.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQSIIDFYNEVGGETHFLSFGKD
                     EYKISLEEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            16702..18687
                     /locus_tag="PC545_000519"
     CDS             16702..18687
                     /locus_tag="PC545_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDF2455311.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKVNDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTINLKGKTYKLKDTYLPTIDKKDPYKLTMEERNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDKMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKRLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            18911..19879
                     /gene="spoIIIAA"
                     /locus_tag="PC545_000520"
     CDS             18911..19879
                     /gene="spoIIIAA"
                     /locus_tag="PC545_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HDF2455312.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKVSNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMTLDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKAIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIED
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            19869..20390
                     /locus_tag="PC545_000521"
     CDS             19869..20390
                     /locus_tag="PC545_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HDF2455313.1"
                     /translation="MQIKIIIIAFLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGGNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            20407..20604
                     /gene="spoIIIAC"
                     /locus_tag="PC545_000522"
     CDS             20407..20604
                     /gene="spoIIIAC"
                     /locus_tag="PC545_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HDF2455314.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            20620..21006
                     /gene="spoIIIAD"
                     /locus_tag="PC545_000523"
     CDS             20620..21006
                     /gene="spoIIIAD"
                     /locus_tag="PC545_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HDF2455315.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSIQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            21091..22296
                     /gene="spoIIIAE"
                     /locus_tag="PC545_000524"
     CDS             21091..22296
                     /gene="spoIIIAE"
                     /locus_tag="PC545_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HDF2455316.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSEDKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLIGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTVYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            22356..22973
                     /locus_tag="PC545_000525"
     CDS             22356..22973
                     /locus_tag="PC545_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HDF2455317.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGNYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNSAKSNNSSNEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLNLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            22985..23539
                     /locus_tag="PC545_000526"
     CDS             22985..23539
                     /locus_tag="PC545_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HDF2455318.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKSDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            23566..24255
                     /locus_tag="PC545_000527"
     CDS             23566..24255
                     /locus_tag="PC545_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HDF2455319.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSYSSKTEDSSNVDKQGGKESADIDIVDSKASKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNSNK"
     gene            24365..24727
                     /locus_tag="PC545_000528"
     CDS             24365..24727
                     /locus_tag="PC545_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2455320.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            24812..25318
                     /gene="nusB"
                     /locus_tag="PC545_000529"
     CDS             24812..25318
                     /gene="nusB"
                     /locus_tag="PC545_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDF2455321.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDKVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            25302..26372
                     /locus_tag="PC545_000530"
     CDS             25302..26372
                     /locus_tag="PC545_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HDF2455322.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVRDNSK
                     GLRQSEAVFQHINNLGILSDRIKSFKDKFNVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     KLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNKFISVHMSGGTTEILLTSKQDSHH
                     NVCDTNLGKIAKISIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGIKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIINDNGDNTNQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVNEVVFAGGVSASKYILRELSMKLRKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            26369..27574
                     /locus_tag="PC545_000531"
     CDS             26369..27574
                     /locus_tag="PC545_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDF2455323.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGVKIIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIKRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RMSKSLTNQIELDQYRLETVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLKLE
                     DEKLVKIGALLHNLSPLATMDRGYSITQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IDKIENKEG"
     gene            27576..27791
                     /gene="xseB"
                     /locus_tag="PC545_000532"
     CDS             27576..27791
                     /gene="xseB"
                     /locus_tag="PC545_000532"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDF2455324.1"
                     /translation="MNLTYEEAYKRLESILSELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            27794..28681
                     /locus_tag="PC545_000533"
     CDS             27794..28681
                     /locus_tag="PC545_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDF2455325.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDEAKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLKGLAEYIIDREY"
     gene            28711..29148
                     /locus_tag="PC545_000534"
     CDS             28711..29148
                     /locus_tag="PC545_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HDF2455326.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGIVTALILM"
     gene            29182..31047
                     /locus_tag="PC545_000535"
     CDS             29182..31047
                     /locus_tag="PC545_000535"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDF2455327.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KENESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYIDTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKELIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSAKVKSMSISVGEKLVDMANSNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     REMELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDDGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEGMLKALLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNIDELYDFVGLSPKSIADKIRKLVIE"
     gene            31180..31992
                     /locus_tag="PC545_000536"
     CDS             31180..31992
                     /locus_tag="PC545_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family rRNA
                     (cytidine-2'-O)-methyltransferase"
                     /protein_id="HDF2455328.1"
                     /translation="MKKRIDLLLVEQGYFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCSIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEVVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNEGYEFDGETYNNKIVEVVEASHNLDK"
     gene            32012..33691
                     /gene="recN"
                     /locus_tag="PC545_000537"
     CDS             32012..33691
                     /gene="recN"
                     /locus_tag="PC545_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDF2455329.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNRYLKKILEENDISMEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYGKIK
                     DRLDEILNRDERVEQLRSKLMNIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGPFTLDGVDDIEFMISFNLGEDIKPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            33906..34772
                     /locus_tag="PC545_000538"
     CDS             33906..34772
                     /locus_tag="PC545_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455330.1"
                     /translation="MNKKDTMHLIFIIVIFILLWYIVVAVSEINKNIESCSNKLDYLS
                     QELMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDIDVQLSKLGKNGKVFF
                     MYKEDSDKWNETEMTKHGELSYACEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFNSGIRDNREYFIEIVENSNLFNHESEKSKNKVYDNIRLEKVDIIVNEGKKD
                     TIYKAKLAEGLDDGKTNNSNRNQVNYEVSFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEK"
     gene            complement(34865..35356)
                     /locus_tag="PC545_000539"
     CDS             complement(34865..35356)
                     /locus_tag="PC545_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455331.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKLLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKFGYTHMRLDTLPSMKSAQGLYEKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(35417..35857)
                     /locus_tag="PC545_000540"
     CDS             complement(35417..35857)
                     /locus_tag="PC545_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455332.1"
                     /translation="MKLNVRIADINNWHDLVSLSVDKSQLDYIESNALSIAESKFITA
                     WVPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGKGYGKASLDFLVNHLKNEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            36018..37076
                     /locus_tag="PC545_000541"
     CDS             36018..37076
                     /locus_tag="PC545_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2455333.1"
                     /translation="MNKINKNFKNIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNHDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNKYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGGAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            37218..38042
                     /gene="spo0A"
                     /locus_tag="PC545_000542"
     CDS             37218..38042
                     /gene="spo0A"
                     /locus_tag="PC545_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HDF2455334.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            38455..39576
                     /locus_tag="PC545_000543"
     CDS             38455..39576
                     /locus_tag="PC545_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455335.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSHQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            39594..40469
                     /locus_tag="PC545_000544"
     CDS             39594..40469
                     /locus_tag="PC545_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HDF2455336.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFDILKEKNDKLDSDLASVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIILKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNFIGDTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            40493..41185
                     /locus_tag="PC545_000545"
     CDS             40493..41185
                     /locus_tag="PC545_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2455337.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDIKQINEIIVVDDGSIDN
                     TAKVVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADIIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            41193..42017
                     /locus_tag="PC545_000546"
     CDS             41193..42017
                     /locus_tag="PC545_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2455338.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            42022..43089
                     /locus_tag="PC545_000547"
     CDS             42022..43089
                     /locus_tag="PC545_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HDF2455339.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPKISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFAASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADAVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLDKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            43091..43627
                     /locus_tag="PC545_000548"
     CDS             43091..43627
                     /locus_tag="PC545_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2455340.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     IKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVYEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            43695..44300
                     /locus_tag="PC545_000549"
     CDS             43695..44300
                     /locus_tag="PC545_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HDF2455341.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPGSSNN
                     ILNNINPAVEYYNLNISIKDTVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILALISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTASIGIIKFLAK"
     gene            44312..45226
                     /locus_tag="PC545_000550"
     CDS             44312..45226
                     /locus_tag="PC545_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2455342.1"
                     /translation="MDIIEGYIDYIKDKKKLSENTVASYFMDIKKYMEYINQKSIKLV
                     DIVENDIISYLIKLEKDNVSIATIARMISSIKSFHDYLFLNHICTNNPAKDIKKPKIK
                     KENINILTEEEIERLLNFPKLTTPKLIRDKAIFEVLYGTGIKVSELVEMNIEDIDLDI
                     DYIYCNSCCKNQRVIPLCDITKLYLEKYLKEARPKMAVEGEKSLFVSSLGQRFTRQGL
                     WKVIKKYSNLANIDKNINPTMLRHSFAIHLLNEGANIAVVSKILGNVNLSSLQVYLNH
                     IDKNVRREIKEKHPRNDVDVELQKAEAK"
     gene            45268..46440
                     /locus_tag="PC545_000551"
     CDS             45268..46440
                     /locus_tag="PC545_000551"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDF2455343.1"
                     /translation="MSRVIWIVIDSVGIGALPDAEKFGDSKDVSTLGNIFKEYPDIQI
                     PNMRNLGIGNIDGIDFFESIKEPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIEEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKEDLF"
     gene            46487..47299
                     /locus_tag="PC545_000552"
     CDS             46487..47299
                     /locus_tag="PC545_000552"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDF2455344.1"
                     /translation="MFEKIAQSSKFINSKSNIKPKIGLILGSGLGDLANDIEDPVTIK
                     YSEIPNFPVSTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEILIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRFGARFPDMSDAYSS
                     KYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQPLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            47503..48828
                     /locus_tag="PC545_000553"
     CDS             47503..48828
                     /locus_tag="PC545_000553"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDF2455345.1"
                     /translation="MRMYDIIKKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFIDSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLISSSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFELA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAHDFEDGVEKIRESIKSGTAIQKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYISKIKAEDVGVSAMILGAGRETKDDILDLSAGIILEKKVGDYVNEGE
                     VLAYMYYNDEQKLSLAKGRFIDSYSIVDSKIEKNKLIYGIVTKEEIKKF"
     gene            49031..50110
                     /gene="mltG"
                     /locus_tag="PC545_000554"
     CDS             49031..50110
                     /gene="mltG"
                     /locus_tag="PC545_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDF2455346.1"
                     /translation="MNFKENRLKIAVLIIVILIILAGIFVFIQIGPYDKNNKKDVIID
                     VPSGASVGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKELNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPEKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            50282..50956
                     /locus_tag="PC545_000555"
     CDS             50282..50956
                     /locus_tag="PC545_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDF2455347.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKVQKPKRILEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFDNNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLVPIGDGVALSYKESKRG
                     DVDGIK"
     gene            50943..52190
                     /locus_tag="PC545_000556"
     CDS             50943..52190
                     /locus_tag="PC545_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2455348.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YRDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEEMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            52262..53926
                     /locus_tag="PC545_000557"
     CDS             52262..53926
                     /locus_tag="PC545_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDF2455349.1"
                     /translation="MSKKKTPFLKKVGKRSWCIFTIILIIYSVLIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKSQILIVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQTKNIDKSMKKELEKNGIMVEEKTMRYAKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTVD
                     NKDKDRHLKTTIDYNIQKKLEQILNKEENPTAAIISEASTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIIANNGTFKPLYLYKSLVDNNMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIK"
     gene            complement(54023..54295)
                     /locus_tag="PC545_000558"
     CDS             complement(54023..54295)
                     /locus_tag="PC545_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2455350.1"
                     /translation="MSLLDILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQ
                     LRYGLTPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
     gene            complement(54330..55847)
                     /locus_tag="PC545_000559"
     CDS             complement(54330..55847)
                     /locus_tag="PC545_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2455351.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQNLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADVVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHDYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKASDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELGKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLNTVKKDNSKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHEDQ"
     gene            complement(55972..56793)
                     /locus_tag="PC545_000560"
     CDS             complement(55972..56793)
                     /locus_tag="PC545_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2455352.1"
                     /translation="MIISKKITAILVSFLIMISMVGCAPKESATEVAKKYLDSIEIKD
                     YEVAYNLLSKDSKESVSLEKFEEYQDTLFKLQTLKDYKLGEENKVEKYKYSNKEYTNL
                     VKIKETDTIKYITENKEDTANFDRYFIIEDNEYKLLWHKDFKRALSSNYVKIARSETE
                     KDEDKDYNKAAIDIKKAIEIDDKNPLAYYTQSYIYIELQRYDEALKSIDKYLEYLDKN
                     NEAYKIDASDAYNLRGVIFMSKSEFNKARESYNKALELNPDNEYAKTNLNDIKGL"
     gene            56984..58011
                     /locus_tag="PC545_000561"
                     /pseudo
     CDS             56984..58011
                     /locus_tag="PC545_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp26"
     repeat_region   58889..59973
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=58889..58917
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            complement(60260..60484)
                     /locus_tag="PC545_000562"
     CDS             complement(60260..60484)
                     /locus_tag="PC545_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455353.1"
                     /translation="MDVNQRIKYIRENMNSSQKELLEKTNINTSVMNRIESGERPTRE
                     DGLIIFAKIFGLYTDYILGLSDTEKLNIGN"
     gene            61163..61318
                     /locus_tag="PC545_000563"
     CDS             61163..61318
                     /locus_tag="PC545_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDF2455354.1"
                     /translation="MALEVKIEVKIIKDVVILNITHWKKETSLLTVKIARGQGDLSYV
                     KYFTNES"
     gene            61564..61815
                     /locus_tag="PC545_000564"
     CDS             61564..61815
                     /locus_tag="PC545_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455355.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            62457..62666
                     /locus_tag="PC545_000565"
     CDS             62457..62666
                     /locus_tag="PC545_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455356.1"
                     /translation="MMGLKLKRRYKNVLIYINIRTIININCKIKFIELYLTFNELKNK
                     LANNEKTTVINENNKAFSGLVDFSN"
     gene            complement(63035..63418)
                     /locus_tag="PC545_000566"
     CDS             complement(63035..63418)
                     /locus_tag="PC545_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455357.1"
                     /translation="MRKKFFIGILIVLIISVIAILNIKNDKNVFIGESENWKAKFVTT
                     EGHSKLEIKYKNSKDVVEQKFNYSYQFIDGEDAVVVVKNEILEAGQEKAIKIQRPGLY
                     SNRESSYKLIISWNGNEETINLKKK"
     gene            63545..63874
                     /locus_tag="PC545_000567"
     CDS             63545..63874
                     /locus_tag="PC545_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455358.1"
                     /translation="MNYRLKKSVTTILSLLIMTILLPTNASASKVEDSSMKVMYSEKQ
                     VTDMDKLYDMAKNGITDVKSSNEKGIIKNARTGESIYVDSISTTQLLEVKQSKNLTSK
                     IYSKTVF"
     gene            64071..64280
                     /locus_tag="PC545_000568"
     CDS             64071..64280
                     /locus_tag="PC545_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455359.1"
                     /translation="MHINQTGFISNGLGIKQQQKKEPTSNSFSYNAPSSWKPVGRDSV
                     QYNCGFESIATLVRGGSSWSFNFIV"
BASE COUNT        26058 a         6810 c        11634 g        20822 t
ORIGIN      
        1 atagagtttt atttagacat ttattacaaa tcaaaattta ttacatatta tttataaaaa
       61 aatctaagga ggaaataata atgaaaatat tagtattaaa ctgtgggagt tcttcattaa
      121 aatatcaatt aatagatatg aataatgaag aagttttatg tataggattg gttgaaagaa
      181 taggaataga aggttctata ttaaaacatg aaaaagcagg tagagatgat aaatacgttg
      241 ttgaacaacc aatgaaggac cacaaagatg caatagcatt agttttagaa gctgttgctc
      301 atccagaatt tggtgcagtt aaagaaatga aagaaataga tgcagtagga catagagttg
      361 tacatgctgg agaaaaattt gcaacttcag tagtaataac tccagaagta gaagaagctt
      421 taaaagaatg tatagattta gctccacttc ataatccagc aaacataatg ggaatagatg
      481 cttgtaaagc tatacttcca gatgtaccaa tggtaggtgt atttgatact gctttccatc
      541 aaacaatgcc taaatcttca tacttatatg gtttacctca tgagctatat actaaatatg
      601 gggtaagaag atatggattc cacggaactt ctcataatta tgtatctcag agagcagcag
      661 agatattagg aaaagatata aaagatttaa aaatagtaac ttgtcactta ggaaatggtg
      721 cttctatagc tgctgttgat ggtggaaaat gtgtagatac atctatggga ttcactccat
      781 tagaaggatt aataatggga actagatgtg gagatataga cccagctata ttaccattct
      841 taatgagaaa agaaggttta gatgctgatg gattggataa attaatgaat aaagaatctg
      901 gagtatatgg aatgactgga atttctagtg acttcagaga tatagaagat gcagctaaaa
      961 atggagacga aagagctcaa gctacattag aagcgtatgt taaaaaagta caaaaatata
     1021 taggcgctta tgctgctgaa atgaatggat tggatgttgt agtatttact gcaggcgttg
     1081 gtgaaaatgg aaaggctata agagcagata ttgcttctaa catggagttt ttaggaatga
     1141 aattagataa agaagctaat gatgttagag gaaaagaaac agtaatatct actgctgatt
     1201 ctaaagtaaa aatgctttta ataccaacta atgaagaatt aatgatagct agagatacat
     1261 taagattggt aaaataatta aattaaacct atgcctatca agtaaaaata cttgacaaac
     1321 aaatgctaga tttgtataat aaatttggtg atatgttgag gtgaacttaa tgaaagttag
     1381 catagaaaaa ataaatagaa aagaaactga caaaatagac ttgaattttt gtgaaaaaat
     1441 tgatactatt agttattgtg atgagattta caaattagta tcacctgtaa atttaaaagg
     1501 gaaagtgtca aagactaata aaggtttgta tctagatata gatgtcaatt ttacaattgt
     1561 tgacaattgt tcaagatgtc ttaaagaagt agaaatacca ctagagtatt ctatacaagg
     1621 ttttttagta aaagaagaag attatgatga agatgagttt gaggaatttg atccttttat
     1681 attcgatggt gaagaaatcg accttattga tataatagaa cagacattag attttaatgt
     1741 acctcataaa gttctttgta gtgaaaactg taaaggtctt tgtcaagtat gcggggcaaa
     1801 cttaaatgaa gaagagtgtt cttgcagtga aattacaaac gatgaggaat acatagatcc
     1861 tcgttttgct aaattaaaag atttatttaa ttaaattttg actaaggagg tggcgtaaat
     1921 ggcagtacca aagcgtaaaa cgtctaaatc aaacacaaaa atgagaagag cagctaactc
     1981 aaagatggaa gcaacaggat ttgtaagttg cccacaatgt catgagccaa aattaccaca
     2041 tagagtgtgt ccagactgtg gatattataa aggtaaagaa gttgtgtcta agtaatagct
     2101 tctaaaaagc atgtagagta tactctacat gttttttttg tacatataaa aaaataagat
     2161 gatttactag agaaaataag tggattttat gcatattaaa aaatagaaaa ataataatat
     2221 aagtattgaa aatttttgtg tgttactata tactactatt agtagctggt actaattaca
     2281 gctataaaat ttggagggaa catgaaaaag aaaagtaaag cccaaagaca aaaagagctt
     2341 atagatatgc taaagacaga tcctttttat actgatgaag aattatcaag tctgtttgat
     2401 gtaagtatac agaccataag attagataga atgagtctaa acataccaga gctaagagaa
     2461 agagtcaaat caatagcaga aactcagagt tcaaaagtaa agactttagg agtaaaggaa
     2521 attacaggag aaatcattga tttatctgta gggcgattag gtatatctat gttagaagtt
     2581 acacaggaca tgatttactc taagactaat actttaaaag atacgtatat tttttctcta
     2641 gcagattcat tggcaatggc tataatagat gcacctaagg ttattatgag agttgcaaat
     2701 gtaaaaagtt ttaagttgat agaacaacaa gatagactta tagcaaaagc agaagtgtat
     2761 agaaatatag ataaaaagca ttatgtaaaa gttgttatca acaataaagc ccaagagcaa
     2821 atatttagag gaaagtttat ctttgaagaa ttagattagg agggaaatta tgaaaatagt
     2881 aattgatgga atgggtggag ataatgcacc taaatctaat gtagaaggcg cagtaaatgc
     2941 tataaaagaa tatcaggttg atttaataat cacaggagat aaagatttac ttgaaaagga
     3001 attttcaaat tatgaatttg atagaaataa attagagata gttcatacta ctgagattat
     3061 agaaaacgaa gataaaccag taaaggctat aagaagtaaa aaagattcat ctatggtagt
     3121 agcattaaat cttgtaaaag aaggaaaggc agatgctata atatcagctg gaaatacagg
     3181 agccttactt gctggtggat tatttgttgt tggtagaata aaaggaatcg atagaccttg
     3241 cttatgttca gcaataccaa atgtaaagcg aggaatgaca cttatagcag actgtggcgc
     3301 aaatgcagat tgtaaaccta agaatctagt tgaatttgca gctatgagta atatttattc
     3361 aagaaaagtt ttaggtcttg aaaatcctaa ggtagcatta gcaaatgttg gactagaaga
     3421 aggcaaaggc aatgatttag tcaaaaggtc atatgaagaa attaaaaaat tagatttaaa
     3481 ttttataggg aatgtagaag ctagagaagt tataaatgca tacacagata taatcatatg
     3541 tgatggattt acaggtaata tattacttaa atctgctgaa ggtgtagcac tttcagttat
     3601 gagtcttata aaggaaactt ttatggccag tacaaaaagt aaaataggag cattacttat
     3661 aaaggatgat ttaagaaaat taaaaagttt tatagattat tctgagtatg ggggagctcc
     3721 acttttaggt ctaaatggag gagttataaa agctcatggt agttctgatg ctaaagctat
     3781 aaaaaatgct ataaatcaag gaataaaatt ttcaaaggga aaagtcgttg aagatattaa
     3841 ccaatttata agcaaataca atgaagaaaa taaaaataac gaagatgaat aaactcgttt
     3901 tggaggttta atatataatg aatacaaagg ctggaatatt aggagtagga agttatctac
     3961 ctgagcaatc atatgataat tttcacttcg aaaaaataat ggatacatca gatgagtgga
     4021 taagtactag aactggtatt aaggaaagaa gatttgccaa agagtctgaa gcaacttctg
     4081 acttagcatc taaagctgcc ttaaaagcta tagaatgtgc aaagttaaac gtagaggata
     4141 tagagctgat tatattagct acaattactc ctgatatgtc tttgccttca actgcatgta
     4201 tagtacaaga tgcaatagga gcagtaaatg ctactgcatt tgatatatca gcagcttgtt
     4261 ctggatttgt atatggagta actattgcaa agcaatttgt ggaaacaggt tgctataaaa
     4321 atgtgcttgt aataggagca gaaacttgtt ctaagttttt aaattatgat gatagaacaa
     4381 cagcagtatt gtttggagat ggagctggag cagctgttat tggccctgta aatgagggtg
     4441 gaatactgtc tacacatatg ggctctgatg gtaaaggaaa agattgttta aaagtacctg
     4501 ctggaggttc aagacttaag gcaagtaaag agactgtaga agctaatcta catacaatag
     4561 aaatggcagg tagtgatgtg tttaaatttg cagttagaaa gatggcagaa acttctttaa
     4621 gagctcttga aaaagcaaat ctaaatacaa ctgatataga ctacttagtg ccacatcaag
     4681 ctaatataag aatcatacaa gcttcttcta agagattaga gcttgatatg aaaaaagttt
     4741 atgtaaatat agacaaatat ggaaatatgt ctgctgcatc tataccagta gcattagatg
     4801 aagcttatag agaaggtaaa attaaaaagg gtgataatgt agttttagtt ggctttggtg
     4861 gaggtctaac ttggggggct tcagttgtca agtggacttt atagtataaa atatctaaaa
     4921 tgatttaatt aacaaaatta aacttataca caaaataaag ttgattaata ataaaaagtt
     4981 tacttttatg atttcaagtg aaggagaatt gtatgaataa aatttgcaaa atattaaata
     5041 taaaataccc agttatccaa gggggaatgg catgggtagc tactgcatca ttagcaagtg
     5101 ctgtatctaa tgcaggagga cttggcataa tagcagcagg aaacgcacca aaagaagcta
     5161 taaagaaaga aattgttgag tgtaaaaaat taacagataa accttttgga gtaaatgtaa
     5221 tgcttatgtc gccatttgtt gatgatataa ttgatttgat tatagaagaa aaagttcaag
     5281 ttattactac tggtgctgga aatcctgcaa agtatatgga tagattaaag gaagctggaa
     5341 caaaggttat tcctgtagta cctacaatag ctttggcaca aagaatggaa aagctaggag
     5401 ctacagcagt aatagcagaa ggtactgaag gtggaggaca tataggagaa cttactacta
     5461 tggtcttagt tccacaagtt gctgatgctg taaacatacc tgtaatagct gctggaggaa
     5521 ttgtagatgg tagaggaatt gcagcatcat ttgcattagg tgccagtgca gttcaagtag
     5581 gaactagatt tatttgcagt gaagagtgtt ctgtccattc aaactataaa aacttagtac
     5641 taaaagcaaa agatagagat gcaattgtaa caggaagaag tactggtcat ccagtaagaa
     5701 cattaaaaaa taaactatca aaagaatttt taaagatgga acaaaatgga gctactcctg
     5761 aagaactgga taaaaaaggt acaggagctt taagatttgc aacagtagat ggagacatag
     5821 aaaaaggttc atttatggca ggtcaaagtg ctgctatggt aaaagaaata acaccttgta
     5881 aggaaattat agaggctatg gtaaatcaag caagagagat tatgccagca atagaactgt
     5941 aaatcaggag gagcaagatg ggaaaagttg cactagtatt ccctggtcaa ggagctcaat
     6001 atgtaggtat ggccaaggat ttatatgaaa ataatactgt tgctaaaagt gtaatagatg
     6061 aggctagtga tgccttaaat atggatttaa aaaaccttat gtttaatgga aatgaagaag
     6121 aactttctaa aactgaaaac actcaacctg ctatggttac acatagtgta gctgtattaa
     6181 aagcagtaca agcccaagta gatttaaaat atagtgcatg tttaggttta tctttaggag
     6241 agtatagtgc attggttgca gcagatgcaa ttgactttaa agatgctgta tgcttggtca
     6301 aaaaaagagg taagtttatg caggatacag ttccaacagg tgttggagct atggcagcaa
     6361 ttttaggtct agatagaagt atattagaaa atgtagttaa agatatacaa ggaggtattg
     6421 tggaagtagc aaattataat tctcctggtc aaatagttat atctggtgaa aatgaaaaga
     6481 tagaagaagc aatggtgaaa tgcaaagaag caggagcaaa aagagctgtt aaattaaatg
     6541 taagtggacc ttttcactca tcaatgctaa gagaagctgg tgtaaaattg gccagtgaat
     6601 tagaaaaagt taacataact aaacctaaag ttgatgtggt ggcaaatgta aatgcagatt
     6661 actataaaaa cgaaggtaaa gacctgctta taaaacaagt aagttcatct gttctttggg
     6721 aagattctat agaaagactt ttaaatgatg gatatgatac ttttatagaa atgggacctg
     6781 gaaaaacttt aaaagcattt attaagaaaa tagcttccaa taaaaaagca aatgtcaata
     6841 tttataatat agatggtata gattctctta atgaatttgt acaaaattat agaaatggag
     6901 agatataatg ataaatctta caggacaagt agcagttgtt actggtggct ctaggggtat
     6961 aggaaaagaa atagcaaaaa aactagcatc ttttggagct gatgtagtaa tcaattatac
     7021 ttctaaagaa gatgaagcac taaaaactaa aaatgaaata gaaagtatgg gggtaaagtg
     7081 tacctctata aaatgtgatg tgtctaaatt tgatgaagtg aatcaaatga tagattctgt
     7141 tgtaagcgaa tttggaaaaa ttgatatatt ggttaataat gcaggcataa ctaaagatgg
     7201 tctgcttatg agaatgaaag aagaagattt tgatagagtt atagatataa acttaaaagg
     7261 tgtctttaat tgtacaaaag cagttactaa acctatgatg aaaaagaagt atggaagaat
     7321 aataaatatg acttcagtag ttggaattat gggtaatgca gggcaaacta attattgtgc
     7381 atcaaaagca ggtgtaattg gatttacaaa agcttctgca agagagttag catcaagaaa
     7441 cataaatata aatgcagtag cacctggatt tatagaaaca gatatgacta aagtactaag
     7501 tgatgatgta aaagaatcaa cactagcaaa cataccaaag aaatcttatg gtaaaccaga
     7561 agatgtagcc aatgccgtag catttttagt tagtgacatg tcaagttata taacaggaca
     7621 agtaataaat gtagatggtg gaatggtaat gcaataataa tttaaaaagt agagaaatct
     7681 attaaaataa aaaattttag gaggtacaca atgtttaata aagttgtaga aataataata
     7741 gagcaattag gagtagaaga taaagaaata actatggaaa catcattaat gaaggattta
     7801 gaagcagatt cattagatgc agtagaaata ataatggcac ttgaagatga atttggtata
     7861 gaaataccag atacagaagc agaaaacttc aaatctatag gagatatagt taagtatata
     7921 gaagctaata aatagtcgag ctttaaaaaa gtggaggtat ccgatgaata aaagagtagt
     7981 aataacagga cttggatgtg ttacgccgtt gggaacagga aaagaagagt tctggagtaa
     8041 tataaaatct ggagtatctg gtatagataa aataactaat tttgatgcta gcacatatca
     8101 aactcagata gcaggagaag ttaagaattt tcatccagaa gaatatataa gtaaaaaaga
     8161 attgaagaga ttagataaat ttgctcaatt tgcaatagta tctgctaaat tagcagttga
     8221 agatgcaaat cttgatttag ataaagttga tagagaacgt tttggtgtta taataggttc
     8281 tggaataggt ggagttgaag ctatagaaac tcaacataaa attcttttgg aaaaaggaaa
     8341 taaaagagta agttcacttt ttgtacctat gatgatagga aatatggcag ctggtcaagt
     8401 ttcaatattt ttaggagcaa aagggcctaa cactaatgta tgtacagctt gtgcatcagg
     8461 aactcattca ataggtgatg cttttaaggt aattcaaaga ggtgatgcag acatcatggt
     8521 agcagggggt tctgaagctg ctgtaacagg tcttgctttt gctggattct gtaacatgaa
     8581 ggctatgtca actagaaatg atgaccctaa aactgcatca agaccatttg ataaagacag
     8641 agatggtttt gtaatgggag aaggagcagg tattgttatc ttagaagact tagaacatgc
     8701 tttagcaaga ggcgctaaaa tatatgcaga agtagttgga tatggtttaa ctgctgatgc
     8761 ataccatatg acaactccag ctgaaaatgg agagggagca gctaggtcta tgaatatggc
     8821 tttgaaagat ggaaatgttc cattagaaga agtagattat ataaatgctc atggaacttc
     8881 aacttattac aatgatttat atgaaactat ggctataaaa actgtatttg gagaaaaggc
     8941 atatgactta tgtgtgtcat caacaaaatc tatgactggt cacttattag gagcatcagg
     9001 tgctatagag gctgttgttt gtgctatgag tatagaagat agttttgtgc ctccaactat
     9061 aaacatacaa gaagttggtg aagatttgga tttagattat gttccaaatc aaggtaaaga
     9121 aaaaaatatt agatatgcac tatcaaattc tttaggattt ggtgggcata atgctacaat
     9181 agtactaaaa aaatatgtat aaataaatat taaatataaa actaaaaaac aagcacttgt
     9241 tttaactgta taaatttata ttagcttaaa tttaaagtct cctaaattat tttaggagac
     9301 ttttttatta agatttaagt ctagcatatg ttgtaaatca ttttgatata ttgtattatt
     9361 aattattaag aggttattta tatatgtaag ttttcattct acaatataca aaaggaggac
     9421 taaaattttg aattttagac taccaaagaa aaggttaccc aaaaagataa tttttatatg
     9481 tataatactt attagtatta ttttgtttat aaagcatgac caaaatggat tgaaagaaag
     9541 agaaattata aataagggaa aatatatgtc tgaaagcctt gtggcagata aggagtatga
     9601 tgaagcaaaa aaagtggtta agacttcttt cgaatctatt ccagaaaaaa aatacaataa
     9661 agtatataat gaagtttggg atatgttaaa gactatttca tatgttccag atggaacgaa
     9721 gatagtcatt gattctttag aaaagatgag agaatcagat gtgaaattat ctgatgaatg
     9781 tagatttaat atagaaaaaa gattagcatc agtgtatata atggataata attattctaa
     9841 ggcagtagat ttaactgtaa agtctataaa gttggctgag aaattgggga ataattatga
     9901 aaaagcaaga ctagatgttg atttggcgag catattttta aagataggtg gctatgaaac
     9961 aggagagaaa ttaataaagg attcttttaa aatagatatt gatgaaggtg aaaaaaatgg
    10021 aatggttaga ttatatgcta ttataaatct agcagaaata tatgtagaag agggcgaata
    10081 tgataaagct attgaaataa gtagcaggat aaaagattat aaaaagtttg taaatacgga
    10141 taattacaat gattttgata taatggcctc tataatgcag tcaaatgcgt atgtgggcaa
    10201 aaataatata caaaaggcaa agactttttt agaaaaagct gattatctta taaaatcaga
    10261 tagaacagtt tatatattag ataaagatat gtactattat atggcaatgg gaaacttaga
    10321 atataaaagc gaaaattata attctgcaat agataattat aaaaaaagtt tagagatatc
    10381 tactaaaaga aagttaaccc aggagaaaat aaaaaattta aataagatat taaatatata
    10441 tgaaaaacaa ggaaatatag aatctttaaa tgagtatcaa aagatgttaa taaatgaata
    10501 taaagagaac aaaagtataa gagattcaga gactagtttc tatataattg ataaagtatc
    10561 aaacgaaagt aagctatttg aaaaaactaa aaaagaaata aaatattata aaatattatt
    10621 tacagtaatt ttaattgcta tattagggtc tactttttta tacaaccgat tgaagtattt
    10681 taaagcacaa aatttacatg atggtcttac aaatgtttat aatagaaaaa gttttgatat
    10741 tatgtatgaa aagtacagag agaaagatga taattttgca cttgtgatga tagatattga
    10801 taatttcaag ctcataaatg ataattatgg acataaattt ggtgacactg ttataaaggg
    10861 aataacaaat accatttgtg ttatgttaga acaagatgat aaagtcttta ggtacggagg
    10921 agaagaattt tctgtattaa taagaaataa atctggtgat gaagtagcag atataattga
    10981 cagtataagg gttgcaattg caaataaaaa gtggaatgat gatgtgactg tgactataag
    11041 tgcaggtgta gctcatgttt cagatagcaa agatgtacta gaagaagctg ataaaaattt
    11101 atataaagca aaacaacttg gtagaaataa agtagtatat aagtaattat atactatata
    11161 aataagaggt ataaatatga aacaatcaat agctatagta actgataaaa taactaaact
    11221 atcatctttc ttagaagaaa atatacgttt ggttttaggt aattatattg atataaacca
    11281 ctattatctg gaagttttaa aagagaatga taaaatacaa ggtgattttg tactagtaat
    11341 gaatgatgat agattaaaca gtatgagaaa gcatttagta caagatagta agattatagt
    11401 gataaggaga acacttaaag aaaatgaaat atatggatta ttttcaatac caaatggaac
    11461 tgaagtattg gttgtaaatg atacaaaaga aactacctta gaaacgataa gcttatttta
    11521 caaaatagga gtaaaaaatt taaagtttac tccatatatt gaaggaaaaa tttataaaaa
    11581 tatagacata gctataacgc ctggagtaaa gggaaaagta ccaagttata taaataaagt
    11641 aatcgacctt ggaaatagat atattgatat atctaccttt atagaaataa taaatggtct
    11701 tagaatagat gttaaagaaa tacgtaaaaa tttaatggca tattctgaga gtttaataag
    11761 tttagataat ggagttaaag ataattatag gcaactgttt ttaaaaatag aagaacaaga
    11821 tgttatattg aatctatcaa aagatggaat aatatttact tcagcagatg gtataataaa
    11881 cacatttaat aaagaagctt taaaaatttt aggtataaat gaaaatccac aaggcaaaaa
    11941 catagaagat atcatttctg aagatttaaa tattttactt gaagacaaag aaataattga
    12001 tgaagtggta tttacaaata aaaaatatat aaatgtaaat aaaaaaagta ttttttctat
    12061 ggggaataaa gctgggatat attataactt acaagaaata acgtatataa aaaaattaga
    12121 acaaaatctt actaataaat taagagaaca aggtcagata gcaagatata catttaaaga
    12181 tataaaaact aaaaataaaa atatggtaaa gtgtgtagaa ttggctaaaa aaatatctaa
    12241 atcagaactt tctgtattaa taataggaga aagtggtact ggtaaagaat tattatcaca
    12301 atcaattcat aatgcttcca atagaagtaa tcaaccgttt atagcagtta attgtgcagc
    12361 agttccagat agtctattag aaagtcaact gtttggatat gaaaaaggct cttttacagg
    12421 agctttaaga gaaggaaaaa agggtctatt tgaacttgct aataatggaa ctatatttct
    12481 ggatgaaatt ggagatatgc ctccattgct acagacaaag ttactaaggg tattacaaga
    12541 aaagcaggtt atgcctatag gttcccataa tgttataaac atagatgtta gggtcatagc
    12601 agcaacaaac aaaaacttac taaagatgat tagagaagga aaatttagag aggacctgta
    12661 ttatagacta aatgtattgc caatagatgt accatcattg aggaatagaa aagaagatat
    12721 aataacactt atgaatttct ttttgaaaaa caatataaga ctttcgccag aagttataac
    12781 tattttagaa aaatataatt ggccaggaaa tataagagag ttacaaaatg ttgcttctta
    12841 tgtaagtcta atgtgtgata gtatggtttt tagagatgac ttaccaccat atataagggt
    12901 agaatgtgct aacaattatg aagatggatt tggttataca agaaaagaat ttgaggaaat
    12961 actgaggatt ctatatgaaa gaagagagtc atcctctgga attggaagag ggtttataat
    13021 cgaaaagctt atacaggaag gtattgagat ttctgaaagc aaagttagaa aggtgctatc
    13081 ttatttaagt gaatgtggat acatatcatc taaatctggt agaggaggaa gtcaaataac
    13141 ctcagagggg ataaaacttt ataataatat aaattaagct aagtatttga tattgaaatc
    13201 acatattttg aatttagata tttaaataat aataaacttt tatatttata gagattaatt
    13261 tatattatgt cagaaagaat attgtaattt ataattaaat attaataggt ttttaaaata
    13321 ggattagata ggtaataggt taagttagaa taacccatta cctattttta tttgtaagaa
    13381 aagtgtacat agaattagaa gtaaaaaata caactaataa aactattgaa aatactagat
    13441 aaataataaa taaaaataat tatagatttt ggcatacata ttgctaatta tagagataag
    13501 acgacaaaat aattgggagg tttaatgatg aaatacaaaa caacagggac ttgtgctaca
    13561 gaaattgaat ttgaagtaaa agaaaataaa gttacaaatg taaactttat aggtggatgt
    13621 gatggaaatt taaaagggct aaaagtatta gttgaaggca tgaatataga agatgtaata
    13681 caaaagttaa aaggaataga gtgtaaaaca aagccaacat cttgcccaga ccaactgtct
    13741 ctagcattgg aaaattatat gaatataaag taatacataa aacaatcgat tagttaggag
    13801 tttaattatg aaaccaataa tagggatttt aggaaattta ataataatgg aaaatggtat
    13861 gtttccaggt cttgaaaggt catatgtaaa taatgattat ataaatgcag tgctaaaagg
    13921 aggaggtagt ccagtaataa ttcctgtaaa tacagataaa gaagttataa aaaaacagat
    13981 tgaaatggtt gatggagtct taatatctgg gggatgggat atcaatcctc aactgtatgg
    14041 agaagaaaca agagaagaaa cgacatttat atatccagaa gtggatgaat ttgatttaat
    14101 tgcaataagt atagcattag aacttaaaaa gcctatatta ggtgtatgta ggggactaca
    14161 aattttaaat gtaagtttag gaggaacttt atatcaagat aataatctta tagaaggaag
    14221 ttatataaaa catacacaat cttctaaaag acatgtagct actcataaag ttgatgttaa
    14281 agaaggaagt attttagagg gaattttagg aaaacaatta cttacaaata gttatcatca
    14341 tcaaagtgtc aatcagcttg gaaaaggatt aaaagccata gcatactcaa aagatggtat
    14401 aatagaagct attgagaaag aggatgaaaa ttttgtagta ggagtacagt ggcatccaga
    14461 aatgatggta gattactgtg ataaaatgga aaaactattt aaatatttta taaatatttg
    14521 ttcaaattta taatatacct tggggggatt gaacatgaac gaaaagaaca aaatgggatt
    14581 aataagtatt atacttttag gtattaatgc agtagtagga gctggagttt ttctattacc
    14641 aggagatgca atgaaatcat ttggtgtagc aagtatattt gtatatatat ttgatatgtt
    14701 gttagtttta tctatggcat tctgttttgc tgaggttgca ggtaaattca ataaaaatgg
    14761 tgctgcctat gtatatacaa aagaagcttt tggagatttt tgtggttttg aagtaggtct
    14821 tatgaaatgg gtaataggat gtatttcttg gggagcttta atagtaggtt ttcctacatc
    14881 attatcagca gtatgggctc cagcaggtga accacatata caaaaaatta taattgtagt
    14941 catgatagtt gggcttacaa ttataaactt attaggtgta tctctatcaa aaattgtaca
    15001 aaatgttata actgtaggta aactaattcc attaatacta tttataggaa taggtatatt
    15061 ttttataaaa ggagttaatt ttacttcttc aacaatggta cctccaggag ctggagcaac
    15121 agagtttgga gcagcagcac ttttaatgtt ttattcattt acaggatttg aatctatagc
    15181 agttgcagca gaagatatgg agaatccaca aaaaaatata ccaatagcaa ttataagtgt
    15241 aatagtaatt gcatcaataa tatatatatt aaatcaagtt gtatgtgttg gtatactagg
    15301 agattcgtta tctagtacat ctacaccagt ggcagatgct gcaagaatat gctttggaaa
    15361 tatgggagct ggattggtta cctttggaac cttggtttct gttggtggaa tttgtatgtg
    15421 tggtgctttt gtaaatccaa gaagctgtgt agctttagct gatgataaaa tgttaccgag
    15481 aatatttgca agaaaagata aaaaaggtac accatatgta gcgataatag ctactatgct
    15541 aatcactata ccaatagctc tatctggaag ttttgctgaa ttagcagcaa taagtgcagt
    15601 tgctagattc atacaataca taccaacatc tttatccgta ttagtgttta gaaagaaaag
    15661 accagaatta gtaggtactt ttaaaactcc ttttggagca gtgattcctc taattgctgt
    15721 atgtgttgga acatggttat tattccaagc atctatgcat caattaatta tgggattagg
    15781 agctttggca attggagtac cattatactt tataatgaag tcttataata gaaaagtcta
    15841 tgatgaaaat ttaaagaaaa tagtttaatt atgttataac aatttattga gttaaaatta
    15901 gacattggca taaaattaaa tacaaataag tatctacaag gctatattaa aatattatta
    15961 attttttatt ttaatatagc cttatattgt tatcaataaa tgaaaaatat tgtaacaata
    16021 gaagaacaat agtattatgt ttatagacaa aaaacttatt aggaggtaaa tttatgccaa
    16081 caataacatt aaaaaatggt gtagacgtac ttattagaga aggtgtaaga gaagatgctc
    16141 aaagcattat agatttttat aatgaagtgg gtggagaaac tcattttctg tcatttggaa
    16201 aggatgaata taaaatatct ttggaggaac aagaaaatgc tatagaatca gcaaaagcat
    16261 ccgataattc tgttaaactg attgcattta tagatggtga aatagttggg attgcaacga
    16321 ttgattctaa tcaaaaagca aaaggaaagc atgttggagt attaggaatt gttgtaaaag
    16381 aaaaatattg gggaattgga ttgggtaaaa ggcttatgtt agaccttata gaatggtgta
    16441 aatcaaatgg aataaccaag aagataactc ttgtcactaa tgaagaaaat tataatgcta
    16501 taggattata caaaaaagtt ggatttgaag tagaaagcat actaaagaaa gaatgttatt
    16561 ataatggtgt ttatactgat ttaataggaa tgtcactttt attgggaata taattagtgt
    16621 acattttatc tcgaaaaaag tatattaagt atggtataat taaaaaagaa tataacttaa
    16681 tataattttg gaggacgaat tatgaaaaat ctgtgtaaaa tatcagatga ttatttaaac
    16741 agcaagctta agtatttaaa gttattatca aaacaatatc caagtatatc aaaagcaagt
    16801 acagaaataa taaatttaga ggctatatta aatcttccaa agggaacaga gcattttata
    16861 acagatgtac atggtgaata tgaaccattt gtacatgtat taaaaaatgg ttctggtgta
    16921 ataaagagaa aaatagaaga attgttttca aatacaataa gagacagcga aaaaaaaatg
    16981 ttagcaactc ttgtatatta tcctgaacaa aagttagacc taataattaa gcaagaggaa
    17041 aatatagatg atttttatag aataaatatt tatagactta tagaactttg taaatatgct
    17101 tctagcaaat atactaggtc taaagttaga aaactgttac cagaaaattt taaatacata
    17161 atagaagaac ttttgcatga gcatgttaaa agtgaacata aagaagagta ttataaaagt
    17221 atagttgaaa caatagttga cataggaata gctaaggagt ttataattgc tatttctaca
    17281 gttatacaaa aattggttgt agatagactt catgtaatag gagatattta tgatagaggt
    17341 ccaagaccag atataattgt cgataagctt attgaacatc attgtgtaga tattcaatgg
    17401 ggaaatcatg atatattgtg gatgggtgca gcctcaggtg aaaaaacttg tatagcaaat
    17461 gcactgagga tatcagctag atatgcgaat ttagacattg tagaagacat atatggaata
    17521 aatctattgc ctttagccac ttttgctata gaaatgtata aagatgaccc ttgtaaagaa
    17581 tttattccaa aggttaatga ccaaagtgta acaacaacag aaaagtcttt aatggcaaaa
    17641 atgcacaaag caataagtat aattcaattt aagcttgaag gtgaggttat cagacgaaga
    17701 cctgaatttg aaatggaaca tagactcttg cttaatatga taaactatga tgaaggtacg
    17761 attaatttaa aaggtaaaac atataaatta aaagatactt atttacctac aatagacaaa
    17821 aaagacccat ataaattaac tatggaagaa agaaatgtta ttgataaatt agtatcttca
    17881 tttagaggta gtgaaaaatt acaaaaacat gtttcatttt tgttttctaa aggaagtata
    17941 tatttaaagg ctaactcaaa tttactgatt catggatgtg taccgttaaa tgaagatgga
    18001 agctttatgt ctatgaatat aatgggaaaa gagtataaag gtaaggctct tatggataag
    18061 atggagtctc tagcaagaga aggatttttc tttaaagata aggcagaaga aaaactgtat
    18121 ggcatggata ttatgtggta tttatggact gggaaatgtt catcattatt tggtaaagat
    18181 gatatgacta catttgaaag atactttata gctgaaaaag agactcataa agaaaataag
    18241 aacccatatt ttaaacttag agaaaatgag atggcttgta aaagattatt tgaagaattt
    18301 gatttagaac ttgatgaatc tcatataata aatggtcatg ttcctgtgga gagtaaaaat
    18361 ggagagagcc caataaaagc taatggtaaa attctcgtta tagatggtgg tttttctagg
    18421 gcttaccaaa aaactactgg tatagctgga tacactttga tatacaattc tagaactttg
    18481 caattagtat cgcatgaacc atttaattct gctgaagaag ccattgcaaa tgaaagtgat
    18541 atcttatcga caactgttgt agtagagcat aaggcaaaga gaaaaatggt gagagatact
    18601 gatgaaggag taaagataca agaggaaata gaagatttaa aactactttt aatggcttat
    18661 aagaaagggc taataaaaga aatgtagatg ttatagaggc acttcatgat tgaagtgtct
    18721 ttttgttaat acttattaaa tatattataa agattaataa tagtagttta tattatactt
    18781 actaagtgaa tccaagataa atgggttcac tttttttatt tataaacatt attaaaaaaa
    18841 tatagatgtt ttggttatat tccttgtcct ttataaatag aattatataa acaaaggagg
    18901 aggacaagag atgaataaac tttctgatga aataataaac tctctttcta caactataag
    18961 agaaaaggta gaaaaagtct caaacaataa tctgaacata gaagaaatta ggttacgctc
    19021 acaaaaacca ttgatactaa atgcaaactc taaagattat ttttacaatc aaaaaactat
    19081 gacacttgat ttaaatcaac aaaactcata tgtagtaaca agagaagatg tagagcaaac
    19141 ttttcaaata atatgtaaat attcaataca ttcatttatg gatgacataa aaaaaggatt
    19201 cataactttg agaggaggtc atagagtagg gttagttgga aaagcaatag tagaagatgg
    19261 acaagtcaaa aatataaagc atatatcatc tttaaatata agggtatcaa gggaaattat
    19321 tggatgctcg gataagattt taagtcatat cataaaggga aaaaatcaga ttaataatac
    19381 tttgataata tcaccacctc aatgtggaaa aacaacttta ataagagata tagtaagaaa
    19441 tttaagtaat ggtaatgaag attatggatt taaaggttta aaagtggctt tagtagatga
    19501 gcgtaatgag atagcaggag cttatctagg tgttccacaa atggatgtgg gaataaggac
    19561 tgatataata gaaacttgcc caaaagactt gggtattaca atgcttttaa ggtctatgtc
    19621 accaaatgtt attgtgacag atgaaattgg aagtgaaaaa gaaataaagg cattatacac
    19681 agcattaaat ggaggtatag gattaataac tactgtgcat ggagattcaa tagaggatat
    19741 acaaaataga aaagaattaa atagacttct ggataaagag ttatttaaaa aggttataat
    19801 tctttctgca aaaagaggag caggaacaat tgaaaaaatt tatgatttag aagaaaagag
    19861 atggtatttt gcaaattaaa ataattatta tagctttctt gataggaagt agttatttga
    19921 taggtgaaca aatatacaaa acttatactc gtagacataa acaattaaac gatttaataa
    19981 gagttttaga aattttgagg atggatttat cttttggact ttatacatta gaagaaatct
    20041 ttaataggat aggtggaaat aaagagtttt gtttttggaa atttttctat caaatatcag
    20101 aaggacttca caatgaacaa agtaaaactt tggaaataat aatatcagaa aatattgatg
    20161 tcttaagtaa agaaacttat ctaggcaata aagaaattga ggaactgaaa aatctaatac
    20221 ttactttagg gaaaagcgat atagagtcac aacaaagaat gatagattta tctatagaaa
    20281 atttgaaaaa acaaacctat gagacaaagg aagatataaa caaaaaaggc gtattgtata
    20341 aaaaattagt gacattcata ggtataggta tatgtattat attgatttaa tatggggagg
    20401 tttattatgg aaatatcatt gatattaaag gttgcaggtg tcggtattct aatatcagta
    20461 ttgaatatga tattggaaaa aacagataga aaagattggg caggtcttac tacattagca
    20521 ggtgtaataa tcgtattggg tatggtaata acagaaataa gtgacttgtt taatactgta
    20581 agaacaatgt ttcaacttta ctaaagagga gggagtaatt tggaaataat gcaattaata
    20641 ggaattgcaa tcatttcaac tacactctgt ttagtaatta aaaaagatag accagagata
    20701 gcaaatttca tagctataat aactggagta ataatactat tgtcagttat gtttaagcta
    20761 aattttatag tagatagtat acaagatttg gctaacaagg ctaacatccc gactatgtat
    20821 atatcattaa taataaaatt aattgggata gcatacttaa tggaatttgc gatacaactt
    20881 tgtaaagatt gtggggaagg aaacattgct tcaaaactag aatttggagg taaaataata
    20941 gttatgtcaa tgtctttccc tatacttttg tccattgtag aaatggtggt gaatataatt
    21001 ccatagatgt gtatcttgga atgaatataa gtatattaga gagcttttgt tgtattgagg
    21061 tttatagagt gaagagaggc atggttgctt atgaaaaaaa gatttttacc aatgattata
    21121 ggttttttat ttactttctt ttttgtaaat atctttgcta ttatgatatt tgctaatgaa
    21181 gtaccaagta gtgaagataa agaatatggt gaaacaaaaa atagcataga taagtatata
    21241 gatggtcaat tagacaagct agatataaat gagattcaag actatataaa taaagaaata
    21301 gttattaatg atgttaattt aaaatcattt gtaaaagatt taattagtgg agaaaaaaat
    21361 atcttagatt tattcaataa agatggattg aagatattga tgtttgatga atttaaagca
    21421 agtttaaagg tagtagcagt aattttggta ctggctctgt tatcatctat tttaaagagt
    21481 ctggaaaact ctttttcatc aggtgcagta agtcaaatag caacatatat aatatttatc
    21541 acaatggtat cacttacact tattggtttt aaagatgtat tacagatttg ctatgatgca
    21601 atagaccata ctgtaggtct tatgcaagtt ataatgccaa tattaataac atttttactt
    21661 ttaattggat tcccaataac gtcgactact ttgaatccta tcttcatagg aggagtaaca
    21721 tttataaatg tattttttaa aaattttcta tttgtatcta ttactgttgc atttggaatt
    21781 ttaataataa acaatttatc aaaaaatata aggctaaaga gatttttctc ctttgtaaaa
    21841 caaataaatt atgtttctat tggagctatg tttactgttt acttaggtct tgtatccata
    21901 caaggattgt atgtaaccag ttttgataaa ttcagtgtaa agactgctaa atttgctata
    21961 ggtaatttta ttcctgttgt aggtggattt gtatctgact ctgtggatat tttattatct
    22021 tcatctcagc ttataaaaaa tatatttgga ggaatagggc ttatcttact tgttggaatt
    22081 tgcttacttc cagtaataaa aattttatca gtaatagtag tgtacaaatt agcagcaata
    22141 attgtagagc ctgttggaga ggatggaata tctaattttt taaatgaagt ggcaaattta
    22201 atgataataa tgctagcatc agtaattgct ataacagtaa tgttctttgt aactgtagct
    22261 atcttgacct cgataagtgt cgtaagtcaa gggtaattta tatatcagtg aaaaataatt
    22321 acatttaatc taatgttttt agagaggagg atgctatgtt agagggcata aaggcatgga
    22381 tagttagtgt tttgatagga gcatttatag taaatatagt agatatgatt ttaccaagtt
    22441 ctaagataaa accttatgtc aacctagttt taaactttat gtttgtattt attgtaataa
    22501 ctccagtagt aggatttttt tcaaaagata tgagtttaga agatagaata ttaaaatcta
    22561 tgggaaacta caacaagcag tatgtagata gtacaaatgc tttggcaaaa gagactggaa
    22621 ataatagtct atccaaggga tatgaggatg ggttaaaaga agtacttaaa ttaaaacttg
    22681 atgaatatgg ttatgattta gaagatatag agcttaatgg ggcaaatata aataatataa
    22741 aaataaaaga aaagaataat agtgcaaaaa gtaataacag ttccaatgaa gaaaataaaa
    22801 ataatagtac taaaagagag gaagaaaaca taaactctaa tgacaaagaa aattctaaac
    22861 aagtcttcaa aaaaggtaca gagtatgggt tgaacctaaa tgaagaaaaa ttaaaaaacg
    22921 acttaataaa ggtgctggat gtttctatag aagatataca aattgataaa taggagttgg
    22981 gagaatgttg aaaaacttaa atgacaaaga taagagaaag gtatattcac taataactat
    23041 agcaggaata tgtgtggttt cattagtatt attatcttgt tttcctagta gcaaaagcga
    23101 taaacaagtt ggaaaaacag agactaataa aaatgctgaa aagcaagtca ctaaagaaca
    23161 agaaaaagat gatttagagt caaaattaac agcaatacta tctaaaattg atggtgcagg
    23221 agacgttgat gtaatggtta catttgaatc aagtgaagaa atacaacctg cttttaattc
    23281 aaataataca acagaaacaa cagaggaaaa agatgcacaa ggtggagaaa gaacagttac
    23341 gacttctagt gaaaataaaa caatgataac ttcaaattca agtgaccctg tagttataaa
    23401 aactacagag ccaaagataa agggagtgat agtggtagca agtggagcaa gtgacccaag
    23461 tgttaaagag acactttata gtgcagtaca aacatcatta caagtagctg gtcatcaagt
    23521 agaaatatat tctaaataaa tacatataac cggggggata aatgaatgaa gtttaattat
    23581 aagggaagag gatttgtaat aataacttta actgcaatgt tagtggtagt aggaacagtg
    23641 aattatcaac taagtaagaa atctttatta gaaacatcaa aagagtttaa ggcatatgaa
    23701 gaagcacagt tgcaaaaaaa tactgatgat agttatagta gtaagacaga ggattctagt
    23761 aatgtagata aacaaggtgg taaggaaagt gctgatattg atatagtcga tagcaaagct
    23821 agtaaagtaa aagaaaaggc aactgagaca agtaaggaaa taaaagctca attatcatca
    23881 gagaaaaata tgaaaaaggc atcttatatt ttagatatga aaatgaatag agaaaaacaa
    23941 agaaatgagt tagttcaaga tttaaatgaa atgataaata atccatctac aacagaagaa
    24001 tctagaaaag aagcttctaa tatgaaatta aatatagtta aaattcaaga aaaagaactt
    24061 caaatagaaa accttttgag tacaaaagga tatgaagaag cgttagttta tataagcgat
    24121 aacaaagtta atgtagttgt aaatgaagct aaattagaaa agaaagatgc agcaaaaata
    24181 tttgatttag ttgcagaaca agccaatgtg aagtatgaaa atattaaact tacaaataat
    24241 aatagtaata agtaaagtat aaacctcaga attaactgag gttattttct tgcaaagtag
    24301 acttgtattt ggtattatta tacttataaa aatagttcct taaaatcagg aggtaagttt
    24361 taatatggaa gataataaat ttggacaagt aaaaatatct aatgatgtaa tagctacaat
    24421 agctggttta gcagcattag aagttgaagg catagaaact acagcaacat taacagataa
    24481 gttgttaaaa aataatggag taaaaataca aatcgaagaa gaggatgtta atttagatgt
    24541 aatggttacg ataaagtatg gaatgtcaat accagataca gcttttaaag ttcaagaaaa
    24601 tgtaaaaaat actgttgaga caatgacagg actgaaggtt tctcaagtaa atatacacat
    24661 tcaaggaatc agctttaaga aggataaagt tgataaagaa gaagcaaaag caactaagaa
    24721 aaattaatat tagccctctg aaaagtcaga gggttttttg ctggtaattg attttatatg
    24781 gataattaaa cttaaaggag aattttgatt gatgaaaaaa gatagggcac aaaagagtac
    24841 aacaagagaa tatataatga aacttatata ccaaataaat attaataaag aagattttga
    24901 aactttagaa gataaagtag ataatttttt aaaagataat tcagaacata ttataaatag
    24961 atataaagaa cttgcacttc aatactcaaa aaatacaaat ttaaagttag aagatactga
    25021 aattgaagat gttatagaca aaaaatatat aaatacagta tgcaaagcat tgaaagaaaa
    25081 tcatgacaaa atagatgagt taataaataa gcatgctaaa aactggacag tagatagaat
    25141 gccaaaggta gatgtttcta tacttagatt gtctgtgtgt gaaatacttt atttagacac
    25201 tcctaataaa gtatcaataa atgaagcagt agaacttgca aaaatatatt gtgatgataa
    25261 gtctcctaaa tttataaatg gtatattggg aagtgttgtt gatgaaattg ggaaataata
    25321 taataattgg aattgatact agctgctata ctacttctat agcagctatc tctttagata
    25381 agaaagttat attcaatgaa aaaataatgt tagaagtaag ggataattca aaaggtttaa
    25441 ggcaaagtga agctgtattt caacatatca ataatttagg gattcttagt gataggataa
    25501 aatcttttaa agataagttt aatgtagaag gagtttgctc atcaaaaaaa ccgagacctg
    25561 ttgaaaactc atatatgcct gtattcaatg taggtcataa ttttggaaaa ttattgtcta
    25621 gtatatatgg ttgtagattt tatgagacta ctcatcaaga gaatcatatt gaagcaagtt
    25681 tattaaatag taaattaaaa aataataata aatttatttc tgttcatatg tctggtggta
    25741 ctacagagat acttttaaca agcaaacaag atagtcatca caatgtatgt gatactaatt
    25801 taggtaaaat tgccaaaata agtattaaaa aagatgataa aagtaagtta tacaataatt
    25861 ttggatataa tatagatata attggaggaa gtaaagatat tagctttggt caacttatag
    25921 atagagtagg aattaagctt ggatataaat ttccttcagg aaagtatttg gatgaaaatg
    25981 ctttaaattg taatcttaaa atagaaagtg gtctgaaaac ttctgtaaga gatgggtata
    26041 tgaatttatc aggattagaa aatcaggtaa ataagattat aaatgataat ggagataata
    26101 ctaatcaaaa agaatatata tctaaattag tattagattc agtagttaga aatatgttta
    26161 aatctctagt gtatttatgt gaaacttata atgtaaatga agtagtattt gctggtggag
    26221 tttctgcaag taagtatatt cttagagaat taagcatgaa gttaagaaaa aagcatatag
    26281 aagcttattt tacagaacca caatattcaa ctgataatgc tgtcgggtgt gctataatag
    26341 gattgaataa ctttttagga gaaagagtat gaaactaaga gctttagata taagtgaagc
    26401 aaactcatat attaaaagaa ttttaataaa cgacccaata ctttctaatc ttaaagttaa
    26461 aggagaaatt tctaatttta aggttcatag tagtgggaat gtatatttat cacttaaaga
    26521 tgaaacatca aagttaaact gtgtaatttt taaaagtaat tttaatcgca atttaaaatt
    26581 agataatggt gtaaaaatta tagctaatgg atatatatca gtctatgaaa gagatggtgc
    26641 gtatcaatta tacattaatg aaattgaaat agagggaata ggaaatttgc acatagagtt
    26701 taatagatta aaggaaaaac ttaataaaga aggattattt gaccctaaat acaagatacc
    26761 tatacctaaa atgccaaact caattggcgt tataacatct ccaacaggtg ctgttataag
    26821 agatataata aatgtaataa aaagaagata tccgaaagtt aatataaagc tttatccagt
    26881 aacggttcaa ggagataaat cagctgaaga aatctgtgaa gcaattagat ttttcaatca
    26941 tatgaagaat gttgatacac ttatagttgg tagaggtggg ggttcaatag aagaactttg
    27001 gtcttttaat gatgaaatgg tagcaagaga ggtttttaat tctcaaattc cgataatatc
    27061 agcagttggg catgaaacgg attttactat ttgtgacttt gtgtctgata tgagagcccc
    27121 aacgccatca gcagcagctg agattgctac tccatcattg gatgatataa actataaact
    27181 tggaaacata aaaagtagga tgagtaagtc tcttacaaat caaatagagc tagaccaata
    27241 taggttagaa acagtattta ataagataaa taactattta gattcgtaca caataaaaga
    27301 taaagttata caattagaca aaatatatga taaaataatt tttggaatag aaaataattt
    27361 aaaattagaa gatgaaaaat tagtaaaaat tggagcatta ctccataatt taagtccatt
    27421 agctactatg gatagaggtt atagtataac tcaaaaaaat ggaaaagtaa taaatagtat
    27481 aaaaggatta aaaataaagg atagtataga tatagtatta aaagatggaa atttagaatg
    27541 tatgatagat aaaattgaaa ataaagaggg atagtatgaa tttaacctac gaagaagctt
    27601 ataaaagatt agaaagtatt ttaagtgaat tagaatctaa aaatgctagc ttggatgaat
    27661 ctttaagcct atatgaagaa ggtataagcc tttataaaca ttgtaataag cttttagatg
    27721 atgctaaatt gaaaataagt aaatttaatc aattgggaat agaagaagac tttaaaatag
    27781 aggaggaata aaattggaat ttaaacaatg tctaaaagaa aaagcaagtt ttgtagaaaa
    27841 agtacttaaa gaatatatgc ctaaagaaga agggtatcaa aaaactgtta ttgaagctat
    27901 gaactatagc ttaagtgctg gaggtaaaag attaagacca atacttacat tggaggcttg
    27961 taaaatagtt ggaggtaatg aagatgaagc tattcctttt gctattgcaa ttgaaatgat
    28021 acatacatat tcactaatac atgatgattt gccagctctt gataatgatg atttaagaag
    28081 aggaagacca acaaatcata aagtatatgg agaagctatg gggattttag caggagatgc
    28141 tttgttaaac tatgcttttg aagtaatgct tgcaggctca atcaataaag aaaatccaga
    28201 aaagtatcta aaagctataa atgaaattgc caaaggtgct ggtatatatg gaatgatagg
    28261 tggtcaagta gtagatgtag aaagcgaaaa taaacaaatt gaaaaagaaa aattagatta
    28321 tatacatatg aataaaactg ctgcaatgat ggtaggctgt atgagagcag gagctactat
    28381 aggtggagca aattctgaac aaatggaaga aataactaaa tatgcaaaaa atatcggatt
    28441 atcattccag atagtagatg atatactaga catagtagga gatgaagcta agttaggtaa
    28501 aaaggttgga agtgatatag aaaatcataa gtcaacatac ccatctcttt taggtttaga
    28561 taaatctaaa gaaattgcac ataatttgat tgatgaagca aagaaaagta tagaaaaatt
    28621 atctgatgat gtagattttt taaagggatt ggctgaatat ataatagata gagaatacta
    28681 aaatatacag ctaaaatcga ggagggaaaa atggattttt tttcggagat ttttaacaat
    28741 ggggctttgg gaataagtct gattgcttgt ttcttagcac aatttataaa aatatttact
    28801 ggtaaggaaa aaagaattga gttatcaaga atacttattt caggaggtat gcctagttcc
    28861 catagttctt ttgttacaag tttagctaca gtggtaggta ttgaaaaagg gtttaattct
    28921 actgattttg ctataattac cgtgcttgcc ttaattataa tgtatgatgc agcaggtgtt
    28981 agacgggctg ttggtaaaca agctactata ttaaatcaaa tggtggcaga tatacagcat
    29041 ggaaagcata tagagcagaa gaaattaaaa gaattaatag gtcatacacc actagaagtt
    29101 tggtttggag ctttacttgg aatagtaaca gctttaatat tgatgtaaat tgaaaattta
    29161 cagaataaag gtgaggataa tatgtataaa tatttagata aagtaaattc tccaaaggat
    29221 ataaaaaata tgagtataga agaaatggat ttacttgcaa aggatataag aaagttctta
    29281 gtaaaatctg tttcaaagac aggaggacac ttagcttcta atctaggagt ggttgagtta
    29341 acattagcac ttcataaagt atttgatagc cctaaagata agattgtatg ggatgtaggg
    29401 catcaatctt atgttcataa aatagttact ggaagaaaag attgttttgt atctttgaga
    29461 cagtttaatg gactaagtgg ttttccaaag gagaatgaaa gtcctcatga tatttttgat
    29521 acaggacata gcagtacatc tatttctata gctacaggta ttgcctgtgc tagggatata
    29581 aaaaaagaaa actatagtgt tatatctgtg ataggagatg gttctataac aggaggtatg
    29641 gcacttgaag ctttaaatca attaggatat atagatacca atatgatagt aatccttaat
    29701 gataatgaga tgtccataga taaaaatgtt ggaggaatgt ctaaatatct gtcaagtatt
    29761 ataagaaact caacagttga gaaaatgaca gatgaagttg ataaaatttt aaatgttaca
    29821 caaacaggtg aaattttatc taagactgca catagattta aagataaact aatgtatagc
    29881 ttttctcctc aggactgttc attttttgat tcattaggta taagatatta tggacctata
    29941 gatggccata atactaaaga actaatagat atattgagaa aagctaaaca caaaaaaggg
    30001 cctgttcttt tacatgtaat tacgaaaaaa ggaaaagggt acagatttgc agaagaacaa
    30061 cctgataaat atcatggagt atcaaagttt gatataaaga caggtgttac atctgcaaaa
    30121 gttaagtcta tgtcaattag tgttggagaa aaacttgttg atatggctaa tagtaatgaa
    30181 aatatagtag caataacagc tgcaatgcca tctggtacag ggttaaacct gtttgaaagt
    30241 gcatatccaa aaagatatta tgatgttggt atagctgaac aacatgcaac aggatttgca
    30301 gcaggtcttg caaaaaatgg tatgaaacct tactttgctg tgtactcatc atttttgcaa
    30361 agggcatatg accaagttat tcatgatgta tgcataacaa aaaagccagt tacttttctt
    30421 atagatagag ctggattagt tggaaatgat ggagaaactc atcatggtat gtttgattta
    30481 agttacttaa attctattcc aaatattgta gtaatggctc caaaagacac aagagagatg
    30541 gagcttatga tggatttgtc attaaaatta gattgtccac tagcaattag atacccaaga
    30601 ggaagtagtt attacctaga taagggagag tatggagaga ttgtactggg taaatatgag
    30661 gtattagatg acggtcaaga tacagtcata ctttgtattg gaagtatggt aaaacatgct
    30721 ttagaggcta aagaaatatt atcaagagaa ggaataaatc cgacaatagt taatgctaga
    30781 ttcttaaagc caattgatga aggtatgtta aaagcattat taaaaaatca taaaaatgta
    30841 gttactatag aggataatat tgtaactgga ggatttggaa gtagaataaa taagtttatt
    30901 atagataatg aatataacgt aaatatactt aatatagcaa taccagaaga atttgtaaaa
    30961 catggcaata tagatgaatt atatgacttt gtaggattat cgccaaaaag tattgctgat
    31021 aagataagga aattagttat tgagtaaaaa tctaatgtag atattgtaaa ttaaaattta
    31081 tataaaatat agaatttatc aattagaata aatatcaagt gtgttacttc aagctacttt
    31141 agttatcatt aaagtagctt ttaacagaaa ggtaattgta tgaaaaaaag aatagattta
    31201 ttattagtag aacaagggta ctttgaaagt agagaaagag ccaaaaaagc aataatggct
    31261 ggattggttt ttgttgataa tcaaaggtgt gataaggctg gtacagaagt aaaagaagat
    31321 tgtagtatag aagtaaaagg aaatccaata ccatatgtca gtagaggtgg tttaaagctt
    31381 gaaaaagcaa tgaaaaactt tgatttgact attgatggaa aggtatgtat ggatataggg
    31441 gcatccacag gtggttttac agattgtatg ctcaaaaatg gagcaataaa agtattttct
    31501 atagatgttg gttatggtca acttgcatgg aaattgagac aagatgatag ggttgtgtgt
    31561 atggaaagaa ccaatataag aaatgtgaca atagaagata caaagcagtt tgctgatttt
    31621 gcgtcaattg atgtgtcttt tatatcgtta aagttagtac ttccaaaggc aaaggagtta
    31681 gtacgtcatg atggggaagt tgtcgcttta attaaacctc aatttgaagc aggaagagaa
    31741 aaagtaggta aaaaaggagt agttagagaa aaatctacac atatagaagt tataaaaatg
    31801 atttctgatt tctcagtcga aaatggattt gagattttag ggttagattt ttctcctatt
    31861 aaaggtcctg aaggtaatat agaatatcta attcatttga gaaatggaaa tgaaggatat
    31921 gaatttgatg gtgagacata caataacaaa atagtagaag ttgttgaagc ttctcataat
    31981 ttagacaaat aggttaaaag ggggcttaga tttgatcctc gaattatata tgaaaaattg
    32041 tgccttagtt gaagaactaa ggttgaatat tgataaaaac ttaaatatac ttacaggtga
    32101 aactggttca ggaaaatcta taataataga tgcacttgga ctttgtttag gtgataaata
    32161 tgataggtct tttttaagaa aaggaacaga taagggactt gttgaggctg tatttttttc
    32221 agacaataga tatcttaaaa aaatattaga agaaaatgat ataagtatgg aagatgataa
    32281 tttattggta ataactaggt tgatatactc agatggtaag agtactgcta gagtaaatgg
    32341 aaggactgtt aaagtgtctc ttcttaaaga aattgcatct acacttatag atatacatgg
    32401 tcaacatcaa aatcaagctt tgtttaataa agatactcat ttaaaatttc tagacctttt
    32461 tggagaaaat gaattggaag agtttaaaat tgcttataaa aatgtgtacc ataagtatag
    32521 tgaagtaaaa aaagctttaa attgcttgac agaaaataaa gatgagatgc aaattcaacg
    32581 agaaatagat ttattaagat ttcaaataaa tgaaattgaa gctgcaaatc taaataagaa
    32641 tgaatatgaa gatttattaa aacaaagaga agtttataga aatagtgaaa agatatataa
    32701 taatctaaat tcgagttata gcaagttaca taatggtgag tataatgtta ttgacctaat
    32761 aggtcttgca tctaaggaac ttaatgacat atctaaatat gacagtgttt taagtgaata
    32821 tagtgatact gtagaaagaa tcatgtatga gttacaagac atatctggtg agataagaaa
    32881 ttacaaagat aatatagact ttgaacctta tgagctagag cagattgagc ttagaatcga
    32941 cgagattaat aacttaagaa gaaaatatgg agacagtata gatgatatat ttgagtatta
    33001 tggaaaaatt aaagatagat tagatgaaat attaaacaga gatgaaagag ttgaacagtt
    33061 aagaagcaaa ctgatgaata tagaagaaga tttaaaaata aaagccagta aattgactaa
    33121 agcaagaata gaagtagcaa ctactttaga aaaaatactt cttgatgaat taaaaagctt
    33181 aaatatgaaa aatgtaatgt tcaaggttaa ttttgaaaaa ggtccattta ctttagatgg
    33241 agtagatgat attgaattta tgatatcttt taacttagga gaagatatta agcctatata
    33301 taaagttgca tcaggtggtg agatgtccag atttatgtta gcgtttaaaa ccatattggc
    33361 tgatatagat gatattgata cattagtgtt tgacgagata gatactggaa taagtgggat
    33421 agcagcacag atagttggtg agaaattgag tgatatagca aagaaaaaac agataatatg
    33481 tataactcat ttacctcaaa ttgctgcaaa tgcagacact cattattgta tagagaaaga
    33541 tacttcaaat aataggacgt ttaccaatgt tagtaagtta aatgaatctc aacgaaaaaa
    33601 tgaaattgcc agacttatag caggtaacaa tataacagaa aaaactattg aacatgcaag
    33661 tgaaattatt gagttggcta aaaaatgtta gtgaaaaaca agcattttaa taggtgtaaa
    33721 gaatttttat agaaatgctt tacatctatt ttttatgatg aaattatttt aaaatatcta
    33781 gtgcctatat tgaagaaaag taggtttgaa aatggaatga actttgtata aatttgtaac
    33841 tttgaaattt ctaaatgctt aagtataata ttatttatta aggtagtagt gaggggaggc
    33901 ataaaatgaa taaaaaagat actatgcact taatttttat aattgtaatt tttattttat
    33961 tatggtatat tgtggtagca gtaagtgaga ttaataaaaa tattgaaagt tgtagtaaca
    34021 aattagatta tttgtcacaa gagcttatga gtactaagtc ggatattagt aatactataa
    34081 atgaagaaat gtcaaaaagc tatataacaa aaagtattga cttaaaagtg aaaaaaatcg
    34141 agaaaaaaga atgtattatt gatatagatg ttcaattaag taaacttgga aaaaatggaa
    34201 aagtattttt tatgtataaa gaagatagtg ataaatggaa tgaaactgag atgacaaaac
    34261 atggagaatt atcatatgca tgtgaaatta agataaatac aggaagtgaa tatgactata
    34321 aagtagttac aagtggagct atatctgaaa gttctgatgt gcaaaaattg actaaatctg
    34381 attatatgcc tgaacaaccc atatttaatt ctgggataag agataataga gaatacttta
    34441 ttgaaatagt agaaaatagt aatcttttca atcatgagag tgaaaagtca aaaaataaag
    34501 tttatgataa tattagatta gaaaaagtag atattattgt aaatgaagga aaaaaagaca
    34561 ctatatacaa agccaaatta gctgaaggct tagatgatgg caaaactaat aattcaaata
    34621 gaaatcaagt gaattatgaa gtaagttttc ctaaaaggga tattgatgac ataatttata
    34681 ttaaagctaa acttacatac aataatggag tagaatatac aaaggatata actgaagata
    34741 taactatggg attacaagat ttagaaaaat aaatacatat ttaaataaat attcaaatag
    34801 atttgttcaa taaaatagag tataaataaa actgtagtag ttttacttat actctattaa
    34861 aaaattataa ttttaattcc atatatctag ccccttcaat tggattataa gtataaggtt
    34921 ctatatcata aaaaccaaac ttttcgtata atccttgtgc acttttcatg cttggaagag
    34981 tatctaatct catgtgtgta tatccaaatt ttttagcttc ttcaatgatt tcttccaata
    35041 gtatttttcc tatttttaat cctctaaatt gatttcttac atataatctt tttaattcac
    35101 atattttacc ttcaagtttt tttaaagcaa cacaacctgc caaattttca tcaacaaatg
    35161 ccaatatcaa tgagcctgaa ggttttttat attttcctgg aagtgttttt aattcattat
    35221 taaagtcttg aaaacataag tctatattta gagaatttga atattcagta aatagaagtt
    35281 ttacgttttc aaggttttct aaatcttttt cttcaacaaa ttgtaaagtt atattttgag
    35341 ccattttatc cgccattata tacacatgtt tatataatga cttcacctcc ttgatatatg
    35401 tatatttttc ttaatatcaa ttagatttta ataccattac tttttcacca ccatagtcaa
    35461 gctctccatt aaactcaaat ccaaaatctt tataaagctt aatagccatt ttattgtctt
    35521 cgaatatact aagatatagt tcatcacagt tatattcatt tttaagatga tttacaagaa
    35581 aatctaatga tgcttttcca tatcctttac cttgatattt tgaatcaatc ataaatctat
    35641 caagccaaac tctatcatct tctaaacgac catacatagc aaatcctatt aaagaatttt
    35701 catcatatat tccaacaggt acccaagctg ttataaattt agattcagca atagaaagtg
    35761 cattgctttc tatataatct aactgacttt tatctacaga caaactaact aaatcatgcc
    35821 aattatttat gtctgcaatt cttacattta atttcaagat attaccgccc ttcaaacttt
    35881 ttgttacaat ctaatactat catattatgt attaaaaatc acttattaag attttaaata
    35941 aactttttaa atgaataaat tctcaggaaa aaggaattat aaatacataa taatacagca
    36001 taggagtaga tggatatatg aacaaaataa ataaaaattt caaaaatatt tggataatat
    36061 taggagctgt atcgattttt accatggtta taatttttaa taaaaattgt aatgttaata
    36121 aagagactca aatagaagct tttacaatta agaatcatga taaaaaattt gtatatccta
    36181 tgggaaatat aattggagta aaagcaaata cagatggtgt attggtctta ggatatgagg
    36241 aagaggatgt agactatatt ggaggcatac aaatcggtga taatatagta aaaatagata
    36301 ataaaaggat aaaaaatagt caagatgtat cagagatttt gaataagatt aaaaaaagta
    36361 aagttgaagt gacttttgag agaaaaaata agtataaaac agaagtaata gagactaaaa
    36421 aagaaaatgg caagtataaa cttggattat gggtaagaga taaaatatct ggaattggaa
    36481 ctatgacttt ttacgaccct agtatggaaa agttcaaagc aattggtcat gccataaaag
    36541 attcggatac taatgaacta ttaaagataa agcaaggata tatatataag ccagaacaat
    36601 taaaaatagt aaaagctagt aatgaaaaag taggtaaaat aaaaggagat tttaatgaca
    36661 gcaatttaat gggtaatttc tcaaataatt cagagttagg tataagtggt aatattacag
    36721 aaaaccataa taaagagttt aatgtagcaa ataaagaaaa gcaattaata gaagttggaa
    36781 gaccgcaaga tgtaaaaata ggaggtgcag taattctttt tgaggataaa aacaaaaata
    36841 taacaagcta tgatataaaa atagaaagta tagtatatga taaaggaaat tatagagata
    36901 tggtaataaa agtagtagat gacaagttat tggaatacac aggaggtatc gtacagggga
    36961 tgagtggagc tccaataata caaaataata aaattattgg tgcaataact catgttttta
    37021 gagataatcc gaaaaaagga tatggtattt ttatagatga aatgataaaa ttgtaggtga
    37081 ggcattaaaa aattttatta ttttatcaat tatctaggag gaatataatt ttggagtgtc
    37141 gaatatgctt tagagtagat aattaggaag caattgtgta aaaagtttag ttttctgtaa
    37201 taagaagatg ttttttaatg gggggatttt tagtggaaaa aatcaaaata gttttagcag
    37261 atgacaataa ggatttttgt caggtattaa aagagtattt gtctaatgaa gatgatatcg
    37321 atatattagg catagctaag gatggaattg aagcattaga cttagtaaaa aagacacaac
    37381 cggacttatt aatactagat gtaataatgc cacatctaga tggattaggt gtaattgaaa
    37441 aattgaatac tatggatata cctaaaatgc caaaaataat agtactatca gcagtaggtc
    37501 aagataagat aactcaaagc gcaataaatc taggagcaga ttactacata gtaaagccat
    37561 ttgattttgt tgtgtttata aacagaatta gagagctagt ttctaataga gttacacaag
    37621 ttgagccaaa acctagacca gttcaagaga ctcaaatgac tagaagtgat tttgttaaga
    37681 atgtaggaaa tatagagaca gaaatcacaa atataataca tgaaatagga gtaccagctc
    37741 atataaaagg atatttatat ttaagagaag caataaaaat ggttattgat aatgttgagc
    37801 ttttaggtgc agtaacaaaa gagttatatc caagtatagc taaaaaattc aatacaacac
    37861 caagtagagt tgaaagagca ataagacatg caatagaggt tgcatggagt agaggaaaag
    37921 ttgacacaat aaatcaatta tttggatata cggtacacaa tactaaagga aaaccaacta
    37981 attcagaatt tatagcaatg attgctgata aattaagact agaacatagt atggttaaat
    38041 aaacaagaca taaaaagtaa ggctttttaa ttaaggcatt ggctataaat gcgtattaca
    38101 agcagcgaaa cggtataacc actagggtta taccgtttcg ctattttaat aaatataaaa
    38161 attttattta ttatttgctt actatatcaa tataataatt ttattatact atggatatag
    38221 tatgtgtctt tacaagttgt aaactgacag tggtttattt tttaatataa atattgactt
    38281 tgatgcaggt aaactttgta tttttaagcg tattatggaa tatgttaaat aaaaaaatga
    38341 tgaaatatag tattgtaaat gccaaagatg caaaacaaac ttaaaacatt tattttattg
    38401 ttaagtaatg ctataatata atgtgatttt aataatgata gtggaggttt aaatatgaga
    38461 gtcgaggccc ctataaaagt agatcgaaaa accaaaaaac ttgctaaaag agttgaaagt
    38521 ggggaaatag cagttataaa tcatatagac atagatgaag ttgctgcaaa ctctttagta
    38581 gaagctaaaa taaaacttgt cataaatgcg gctccttcta taagtggtag gtatcccaat
    38641 aaaggtccag ggatattaac tgaaaataat atactaataa ttgataatgt tggagaagag
    38701 ttatttgaag aattaaaaga aggagaaact atagaagttg tagatggaaa gatatataga
    38761 aaaggaaaat ttttaggtgc aggagaagtt ttagacaagt atgaggtttc acaccaaata
    38821 aaggctgcct atgagaattt agctgtagaa ttggatagat ttatagataa tactatagat
    38881 tatgctaaaa aagaaaaagg atttatactt ggagaagtag aaattccaaa agtgaaaact
    38941 aattatgcta ataaacatgt attgatagtt gtaagaggtc aggactacaa agaagattta
    39001 agtactatgc tttcttatat agaagaggta aagccagtat tggttggtgt tgatggagga
    39061 gcagacgctt taattgagtt tggatatact ccagatgtga ttgtaggtga tatggatagt
    39121 gtaagtgatg aagctttaaa aaaggctagt gaaatagtag tacatgctta tacagatgga
    39181 agagcaccag gtcttaaaag ggttgaggaa ttaggcttag atgcagttgt atttcctgca
    39241 ccaggaacta gtgaagatat agctatgctt atcgcttatg agtataaggc tgagttaata
    39301 gtagcagttg gtacacattc aaatatgatt gattttcttg aaaaaggaag aaaaggtatg
    39361 gcaagtactt ttttagttag actaaaaata ggttcaaagc ttatagatgc taaaggcgtt
    39421 aatttgttat acagaagtaa attaaaaata aagtacatat gggcattaat agcaacagca
    39481 ttatttcctg tgttggtagt tgcatcattg tcaccaggag ttcaacagtt catacaacta
    39541 atgcaattaa agtttagagt attattacag atgtaaattt taggaggata aatatgcata
    39601 ttaatatgaa atattatata gtcactatag gggctatatt tatagctttg ggtataggca
    39661 tgttagtagg atttaattta aataataatc aacaattaag tgaacaacaa gcaaacataa
    39721 taaatgattt ggatgataag tttgatatct taaaagaaaa aaatgataaa cttgacagtg
    39781 acttagcaag tgtaaataga gattatgaag aggcagttaa ttttataaat aaaaatgtag
    39841 ataagatttt agctggttcg ctgaatggta aaagtatagg aataatatct gctaatgaaa
    39901 atgacgatta tacaaaaaat atagaagaca taataaataa atctaatgga agcattgcat
    39961 ttaatataat attaaaagaa aacataacaa atccagaaaa gttaaaggaa atgtcaacta
    40021 aactaggtac agatgtaaaa aatgcaaatg atgcagttaa ttatataatt gacactctta
    40081 aaaaggaaga tgcaagtgac attttaacat atcttcaaga actagatgta ataaagttta
    40141 attttattgg agatacatat ctaaaatatg attccgttgt aatagctggt ggaaatgatg
    40201 caaaagacag tacaaaacag tttgaaaaaa tagaaaaatt tgtagtttca aaacttaaat
    40261 cagagaataa atatttagta gaagttcaaa atacaggagt taaaacttct tatgtagaac
    40321 tatattctaa aaacaaagtt gcaactatag acaatattga tgaaggtata ggaagtatat
    40381 ctctagctat tcttctacaa caaggtaata tagttggtaa ttttggtaga ttggatactg
    40441 caacttcatt gttgccatct attaaataga aataatttta aggagagaaa aaatggattc
    40501 ttatataagt ataataatac cagcttataa tgaagaaagt agaataaaat atacattaga
    40561 aagcatattg gacattaagc aaataaatga aataatagtt gtggatgatg gttcaattga
    40621 caatactgct aaggttgtat ctgagctaga aaatgaaaag attaaatttt ttaagttaga
    40681 taaaaataga ggtaaaggat atgctttaaa ttatggtatc aaattagcaa tgaaaaatgc
    40741 agatattata ggttttctgg atggagattt aggaagttct gctaaagaag ttgaaaaatt
    40801 aatagtacct atattaaatg gtgaggcgga tgttacaata gccaagtttc ctcctgcaaa
    40861 gagaaaagga ggtttgggat ttgtaaaagg tttagctaaa aattctgtgt ttgagatgac
    40921 tggtgttgag ttagatgcaa ctttatcagg tcaaagaata tttaaaagag aagttttaga
    40981 aaagtttgat gaaatacctt ttggatatgg agtagaagtt ggaatgacca ttgatatatt
    41041 aaagtatgga tttaaaatta aagaagtatt agtaaatatg actcataatg aaacaggaag
    41101 aaatttaaaa ggttttattc atagagggaa acagtattat cacataaaaa aggttttagg
    41161 acaaaaaaag aaagaatgga ggtaatatct tagtgaaaga ttatatactt tataccattt
    41221 tattattaac tggattattt ggaacctatg ctgtaattcc actatttaaa aatttattga
    41281 taaatggaaa tgtacttaga ccaaactata aaaaagatat gataccagta agtatgggaa
    41341 ttgtattttt gcctatgatt gtaatcaatg gaataatact tgctttcttt actacagaat
    41401 ttaaggattt agcgtatata tttatgttta tgtttggtat gatatccatg ttttttgcag
    41461 ggatactaga tgatgtcatt ggaaatagag atgtaagtgg tttaaagggg cattttaaaa
    41521 gtttatttaa aggaagtctt acaacaggag gttttaaagc tttatttggt ggatttgtag
    41581 ggcttatagt ttctgttgct atatcaaaag acataattga tataatagta aatacactaa
    41641 taatagctct atcaactaat ttaatgaatc ttttggattt aagaccagga agggcaataa
    41701 aagcatattt attcataatg gtaattatat ttttaacatt gacaggattt gttcaagttc
    41761 ttccattact tatagtacca aatgtacttg catactttaa ttatgattta aaagcaaggg
    41821 ctatgatggg agatactggt tcaaacgtcc ttggaatatc tattggaatg ctcatttctt
    41881 ttgggtatcc atttaacatt agattaggat ggttaatatt tgtagttatt atgcatatat
    41941 tcactgagaa atactcatta actaagataa tagaaaaaaa taaactatta aattttatag
    42001 ataaattggg aaggtagtat tatgataagc aaaagagtgg ggattgttga atcaatagta
    42061 agtcaaactg aaacactaga tgatataaga gtaaatataa atggagaaat acaaagggca
    42121 tataattatc ccaaaatatc tggaactata aatataggtg acgaagtggt tttaaataca
    42181 acagcagtag aactgagctt aggtacaggt ggataccatt ttgtaataac taatttaaat
    42241 aatatagaat ctactttaac tgaaggtgga catataatga aacttagata tacgcctctt
    42301 caaataaaag tggattctgt tgaggaacaa gaaagtattt atcatgataa atttgcgaat
    42361 tttaaaggat tagaaggtct ttctgttgta gtagggactc ttcatagtat gttaactcca
    42421 tttgcagctt catttaaaag aaataatcca aataaaaaat tagtgtatat aatgactgat
    42481 ggagcatctc ttccaattta tttaagtaaa aatgtggata ctttaaaaga aaaaaaatta
    42541 atagatagta caataactat aggaaatgcg tttggtggag attatgaatg tataaatata
    42601 tatactgcat taattacagc aaaagaaata ctaaaagcgg atgcagtatt tgtcagtatg
    42661 ggacctggaa ttgctgggac aggaacaaaa tatggattta cagggattga acaaggctct
    42721 attcttgatg ctgtaaaaaa acttgaagga agagcaattg caattccaag aattagtttt
    42781 gctgacaaac gagaaagaca tcaaggtatt tcacatcata gtatgactgt atttaaagaa
    42841 attgtaaatg taaatgtaga cataccaata actatatatg atgatgaaaa attgaacttt
    42901 ataaaagagc aaatcaattt aaatggttta gacaaaaagc ataatataat ttatatagat
    42961 aataataaaa cgaaagagga tttagactat tttaatctaa aagtaaagag tatgggaaga
    43021 aattatgaac aagatgaggc atttttcaaa gctgcaagca cagcagcata ttacttaatg
    43081 gaggtttaag atggtattag aagaaaaaac tattagtagt gatagggttt atacaggtaa
    43141 agtaattact ttaaaggttg atactgttga aattccaggg caaggatatc aaaaaaggga
    43201 gttagtagaa gttggtggag cagtaggaat agttgcaatc acagatgata ataaagttgt
    43261 gttagttaag caatttagaa aacctataga aaagccaatc tttgaaattc cagctggtaa
    43321 actagaaaaa aatgaaagtc caaaagaatg tgctgaaaga gaattaaaag aagaaacagg
    43381 gtatagtgca aaaaatatta agctaataca taaatttttc acttcagcag gtttttctaa
    43441 tgagataatg tttgtatatt tagcaacagg tcttactcca ggagagaata atttagatgc
    43501 agatgagttt ttagatgtct atgaaattga gcttgaagaa gcatataaca tggtattaaa
    43561 aaatgacgtt gaagatgcaa aaacatctat tggattatta ttagtaaaag atatgtttaa
    43621 aaattaatat ttataaaatg aataaccctt tgtcctaaag catattattt tataaaggac
    43681 aagggggaat taaattgaga agaacatata gaaaaaatga ttttagagaa ttaaatagac
    43741 aaataattat tataggattg ttgtttatga tgtctattgt tgttgggtct tacataaata
    43801 aaatattgcc aggttctagt aacaatatat taaacaatat aaatcctgct gtagagtatt
    43861 ataatctaaa tataagcata aaagatacag tgatacaaaa tttaaaatca gatgcaatct
    43921 ttatgggctc aatagctcta ttaagtctat ttgtagtaac aattccagca gtgttggtag
    43981 cttttgtatt aaaagggatg tcaattggtt atacaataaa tagttgtata ttggcattaa
    44041 aatttaaaag tataaaaatg attttaataa tattatttaa aaatttgatt attattccat
    44101 gtgcaatcat attagcatta attagtttaa gttattttaa agaaatggtt tatgaattta
    44161 aaaagaaaaa tagaaaaaat atgcaatttt taattaagag gtatatacta aatattataa
    44221 ttataatagc actatcattg gggttgcaat taatattaaa tactgcaagt attggtatta
    44281 ttaagttcct agctaaataa aggagtttat aatggatatt atagagggat atatagatta
    44341 cataaaagat aaaaaaaaat tatcagagaa tacagtggca tcatacttta tggatataaa
    44401 aaaatatatg gaatatataa atcaaaaaag cattaaacta gtagatatag tagaaaatga
    44461 tattataagt tacttaataa agttggaaaa agataatgta tcaatagcta caatagcgag
    44521 gatgatatcc tctataaaat cttttcatga ttatttattt ttaaatcata tatgtacaaa
    44581 taatcctgca aaggatataa aaaaacctaa aataaaaaaa gaaaatataa atatacttac
    44641 agaagaagaa attgaaagac tattaaattt tccaaagtta acaacaccta aattgattag
    44701 ggacaaggct atttttgaag tattatatgg tactggtata aaagtatctg aattagtaga
    44761 gatgaatata gaggatattg acctagatat tgattatata tattgtaatt catgttgtaa
    44821 aaatcaaaga gtgataccac tttgtgatat tacaaaactt tacttagaaa agtatctaaa
    44881 agaagcaaga cctaaaatgg ctgtagaagg tgaaaaatca ttattcgtaa gttcattagg
    44941 tcaaaggttt acacgacaag gtctttggaa agtcataaaa aaatattcta atctagccaa
    45001 tatagataaa aatataaatc ctacaatgct tagacattca tttgctattc atttgttaaa
    45061 tgaaggtgct aacattgctg ttgtaagtaa aattttagga aatgttaatt tatctagttt
    45121 gcaagtatat ttaaatcata tagataaaaa cgtgagaaga gaaataaagg agaaacatcc
    45181 tagaaatgat gttgatgtag aattacaaaa agcagaagct aaatagacta tatagcaaat
    45241 tttataattt agaaggagca tatttatatg agcagagtaa tttggattgt aattgatagt
    45301 gttgggatag gagcattgcc tgatgctgaa aaatttggtg atagtaagga tgtaagtacc
    45361 cttggaaata tatttaagga atatccagat attcagattc ctaatatgag aaatttaggt
    45421 ataggaaaca tagatggtat agattttttt gaaagtataa aagaaccaat aggttgtttt
    45481 ggaaagtgta aggaaatgtc tcagggaaaa gatactacta caggtcattg ggaaatgact
    45541 ggtataatag ttgacaaacc atttaagaca tttgaacatg gattctctaa ggagataata
    45601 gaagagtttg agaaaaaaac tggaagaaaa gtagtaggaa ataaaccagc ttcaggaact
    45661 gtaataatag atgagtatgg agagcatcaa ataaaaactg gagatgtaat agtatataca
    45721 tcagctgata gtgtatttca gattgctgct aatgaagaag taattcccct agaagaactc
    45781 tataatatgt gcaaaatcgc tagggaaata atgatggggg ataatgcagt agctagagta
    45841 attgctagac cattcatagg taagaaaaaa ggtgagtttg tgaggacttc aaatagaaga
    45901 gattattcac tagacccatt tgaaccaaca gttcttgata atataaaaga atcaggactt
    45961 gatgttttag cagttggaaa aatagaagat atttttaatg gaaaaggaat tacagatgct
    46021 atccacacta aaagcaatat ggatggagtg gatgaaactt taaattatat gaaacaagat
    46081 aataaaggtc taatatattc aaatctcgtt gattttgatt ctaaatatgg tcataggaga
    46141 gacccagaag gatataagaa agctcttgaa gagtttgata gtagacttcc tgaaataatg
    46201 gctaatatga gagaagatga tattttaata atcaatgcag accatggaaa tgacccaaca
    46261 tataaaggta cagaccatac aagagaatat atacctgtta tgatttatgg caataagatt
    46321 aaaaaaggtt ttaatttagg agtaaaagat acttttgctg atattggagc aactgttgca
    46381 gatatactaa atgtaaagtt gcctaaacat gggtcaagct ttaaagaaga tttattttaa
    46441 actataaaat gataaaaact tagcttaata ggatggagag attggaatgt ttgaaaagat
    46501 agcacaaagt agtaaattta taaattctaa aagtaatata aaacctaaga ttggtttgat
    46561 attaggttct ggactaggtg atttagcaaa tgatatagaa gaccctgtca caattaaata
    46621 tagtgaaatt ccaaacttcc ctgtatcaac agtagcagga catgctggac aacttgtaat
    46681 tggaaaactt gaaggaaaag aagttattgc tatgcaaggt agatttcatt actatgaagg
    46741 atattctcag aaagaagcta cttttccagt aagggttatg aaagaattag gagtcgagat
    46801 tcttatagta actaacgctg ctggaggcgt aaataaagaa tttaaagcag gagatttgat
    46861 gcttataaga gaccatataa actttagtgg aagtaatcca ttagtaggaa aaaatgatga
    46921 cagatttggt gctagatttc cagatatgtc agatgcatat tcttctaaat atgtagatgt
    46981 agttaaaaac tgcgctaaag aatgtaatat agatgtcaaa gaaggcgttt atatgttttt
    47041 tagtggtcca aactatgaaa caccagcaga agtgagaatg gcacaaattt tgggagctga
    47101 tgctgtaggt atgtctactg ttccagaagt catagtggct tctcactcta atataggagt
    47161 tattggtata tcttgtataa caaatatggc tgctggcatc ttaaatcaac cactaagtca
    47221 tgaggaagta atagaaacta ctcaaaaagt taaatcagaa tttatgaatt tagttaaaac
    47281 aacggttaaa aatttataag ttaaagctta ttatagattt tttgaggaag tttataataa
    47341 aatagttaat gtttaatttg aaaagaatta ataaaaagct gtcttgatat agaaagttat
    47401 ttctaataaa ggacagtttt tttataatac tacaacattt gaaagtgttt taaagtttaa
    47461 tatttattta aattattata ggttagaatg gagatttcaa ttatgagaat gtatgatatt
    47521 ataaaaaaaa agagagataa cctagagctt agtaaaaaag agataaacta ctttatagaa
    47581 aactattcaa agggagatat acctgactat caagcttctg cactattaat gtcgatttac
    47641 ttaaataaga tgaataaaca ggaaactgta catcttacag aagcaatgat gaactctgga
    47701 gacatgataa acctatctga gatagatgga ataaaggtag ataagcatag tactggtggt
    47761 gttggagata agactaccat agctcttata cctttggttg cttcatgtgg agcaccagtt
    47821 gcaaagatgt caggtagagg tcttggtcat actggtggta ctattgataa attggagtct
    47881 ataccaggtt tttcgacaga aatggagata agtaaattta ttgattctgt aaataagtct
    47941 aaaatagcag tttgtggtca aagtgcaaag gtagcaattg cagataagaa aatatatgct
    48001 cttagagatg ttacagcaac tgttgataat atatcattga tatcatcaag tgtaatgtct
    48061 aaaaagttag cttcaggagc tgatgcaatt gtgctagatg taaaaactgg tgatggagca
    48121 tttatgaaga cattagaaga gtcttttgag ttagctaaat ctatggttga tataggtact
    48181 ggtatgaata gagatacaat agggattgta acagatatgg atgaaccttt aggttttgca
    48241 gtgggaaact ctttagaagt tatagaggca attgagacat taaaaggaaa tggacctaaa
    48301 gaccttgttg agctatgcga agtattagga gcatatatgt tggttttatc aaaggtagca
    48361 catgattttg aagatggtgt tgaaaaaata agagaatcaa taaaatctgg tactgctata
    48421 caaaaactca aagagtttat agagaatcaa ggtggtaata aagatgtagt agatgattat
    48481 tctttattca aaaaatcaaa gtataaatat ccagttaaat caactaaatc aggttatata
    48541 agcaaaatta aagctgaaga tgttggtgta tcggctatga ttcttggagc aggtagagaa
    48601 actaaagatg atattttaga tttatcagca gggataattt tagaaaagaa agtaggagat
    48661 tatgtaaatg aaggtgaagt tttagcttac atgtattata atgatgaaca aaagctttca
    48721 ttggcaaagg gaagatttat tgattcctac tctattgtag attcaaaaat agaaaaaaat
    48781 aaattaatat atggtattgt aactaaagaa gagattaaaa agttttagaa aagtgattat
    48841 aaaaggccta acatgtcaaa aataatgtta ggttttttat tttttgataa actataaatt
    48901 ataccaaaaa tatatatttt aaaacatttt ttacaatata aataagttta ttctacaaga
    48961 tgattgaaaa ttaaagtatc tagttttata atagaaaatg gataaatata cataaggagt
    49021 agattatatt atgaacttta aagaaaatag attgaaaata gcagtattaa taattgttat
    49081 tttgattatc ttagcaggta tatttgtgtt tatacaaata ggtccttatg ataaaaataa
    49141 caaaaaagat gtaattatcg acgttccaag tggagcatct gttggaaaaa tatctgacat
    49201 attatatgaa aataaattaa taaagaatga actattattt aaacttttgg taaaggttag
    49261 caataaagct ccgtctataa aatcaggaac atacttactt aatcaatcgt attcaaacaa
    49321 tgatataata agtcttttag tttcaggtaa aatatatcag gatggtataa aagtgactat
    49381 tccagaggga gctacatcta aagaaattat agcaatgtta gtaagtaaaa atttaggaga
    49441 taaagctact tttgaaaatt taataaaaaa acctcaagaa ttttatgata aatttccata
    49501 tttgaaggaa gatggaataa cctctttaga agggttcttg tatccagaaa cttattattt
    49561 taattcaaag aaacaatcag aagaagatat tttaagcgaa atgttaaaag tatttgacag
    49621 taaatacacg gacaagttta aaaagaaaca aaaagaatta aatatgactc ttcaagaagt
    49681 aatggaaatg gcatcaataa ttgagaaaga agctgttctt gacaaagaca gacctataat
    49741 agcaagtgtg ttttataata gacttaaggt aggtatgcct cttcaatcag atgctacaat
    49801 acaatatata tttgaagaga gaaagaaaat tgtaacatat gatgatttaa aaattgattc
    49861 accatataat agttataaaa ataagggatt acctcctaca ccaatatcaa atcctggtat
    49921 aaaatctatt gaagcagcat tatatccaga gaaaacagac tatttatatt ttgtagctaa
    49981 gattgatggt ggaaataatt atagtacaaa ctatcaagac catttaaaat acgttaaaga
    50041 atataaagaa gcaagggata aacaatcaaa agatacaaaa gcaacaaata aagaaaatac
    50101 aaaaaaataa accatgatta taaaaagtct aacagggtaa gaataatgtt gggctttttt
    50161 attgaaaatt gttattctta gatttataat aaagaaagtg taaatatata taacaatatg
    50221 aatttaaaaa ataataaatt aatataaaac ttgaatattt attattttag ggagtgtttt
    50281 tatgagtaat atagttaatg atttggttga agattatata agagtgacat taaaagaaaa
    50341 agaaggattt ttaaaagatt tagaaattta tgcagaggaa aatagtgttc ctataataca
    50401 taaagaagtt tcagatttat taaaagtctt gttaaaggtt caaaaaccaa agagaatttt
    50461 agaagtagga tgtgcaattg gttattcttc tatttttttt gcaactatta taggaaaaga
    50521 tgcagatata ataactgttg agagaaatga aaaaatgata gaaaaagcaa aagaaaacat
    50581 aaaattagct ggatttgaca ataacataac tatactagaa ggagatgcag aagaaaaact
    50641 tagtcaagtc caaggtgaat ttgaccttat atttatagat gcagccaaag gtcaatataa
    50701 gctatttttt gatttagtta tagataaatt aaaagatggg ggacttttag tatctgataa
    50761 tattttgtat aaaggtatgg ttgctcatga tgattttgtt gtaagaagaa aaaagactat
    50821 agttaaaaga atgagaaatt atttagatta tatatgtaat tgtgactatt taagcacatc
    50881 attggtacca ataggtgatg gtgtagcact aagttataaa gaaagtaaaa gaggtgatgt
    50941 agatggaatt aaataaagta gaacttttag ctccagcagg tgatttggag agattgaaaa
    51001 tagctataac atatggagca gatgccgttt atataggtgg agaaattttt ggaatgaggt
    51061 ctgcagcgaa gaattttagt aaggaagata tggcagaggg agtagctttt gcacatgaaa
    51121 gaggcaagaa agtattcgta acagtaaata taatacctca taatgaagat tttctacaat
    51181 tagaagacta tttgttggaa ctagaagaga taggaataga tgcagttata atagctgacc
    51241 caggagtgct tagtgtcatt aaaaaagtaa ttccgaatat ggaaatacat ttaagtacac
    51301 aagcaaatac aaccaattat ctaagtgcaa acttctggta cgagcatgga ataaaaagag
    51361 ttgtagtggc aagagaatta tcttttgatg aaatatctga gataagagca aaaacacctt
    51421 tagatatgga tatagaagcg tttatgcatg gtgctatgtg tatttcttat tctggcagat
    51481 gtttaataag caactatatg actggaagag atgcaaacaa aggttcttgt gctcaatctt
    51541 gtagatggca atatcatttg gtggaagaaa aaagacctgg tgagtatttt ccaatatacg
    51601 aagatgaaag gggtacattc ttctttaact caaaagattt atgtatgata gagtatatac
    51661 cagagttaat caaatctggt ataacaagtt taaaaataga aggtagaatg aagactgcat
    51721 actatgtagc tactgttgta agagcataca gaatggcaat agatgaattt tacagagacc
    51781 cagaaaactg gaagttcaat ccaatgtgga tggaagaact aaaaaaaggt agtcatagac
    51841 attttacatc aggtttttac ttgaataaac caacaacaga agaccaaaat tatcaatcag
    51901 catcatatgt tagaaactat gattttatag gtatagttag agaaacagaa gatgaagatg
    51961 gtcttattgt tgttgagcaa agaaataaaa tgtgtgttgg tgatgaaata gaagttatgg
    52021 gaccatataa agaaactatg tttactaaaa ttgaagaaat gtacaatgaa gaaggagaag
    52081 ctatagaatc agctcctcat cctagacaaa tagtgaagtt aaaactatca gtaaaagttg
    52141 gtaaagatta tatgcttaga aaagttatag aagaaaaggt tgaagaataa taaaaaattg
    52201 ataaaaatag tattagcccc ttagaaattg tcaataatga aattatctaa ggggtgattt
    52261 catgtcaaaa aagaagactc catttttgaa aaaagtggga aagagaagct ggtgtatatt
    52321 tactataatt ttaataattt atagtgtttt aatttatagg ctagttgata tacaagtatt
    52381 aaaaggtgat aaatacaaac aaagtgttga atcacaaagc gttgagaaag tcgagttaaa
    52441 tagtggaaga ggaataatct atgatagaaa caacaagaag cttacagata caagtaaatc
    52501 tcagatatta attgttgaaa aagaaaaatt aaataataac tataaaattt tggaacttat
    52561 taagaaagct actaagatga atgatttaga tatatataaa gctgtacaag agcaattgac
    52621 acgccctata atacagattc agactaaaaa tatcgataag agtatgaaaa aagaacttga
    52681 gaaaaatgga ataatggtag aagaaaaaac tatgaggtac gcaaaagatg gtttattatc
    52741 acatacaata ggatatataa aagaagatga taaatcaggt caatcaggaa ttgaaaagag
    52801 tatggatagt gtacttagaa attcaaatga aaagtatata agtgctttta aagctggaga
    52861 tgcaggaaat gaaaaatctc taaacatact aaagggaagt gtaaaaactg ttgataataa
    52921 ggataaagat agacatctaa aaactactat tgattataac attcaaaaaa aattagaaca
    52981 aatactaaat aaagaagaaa atcctacagc agcaataatc tcggaagcat ctacaggtga
    53041 aattcttgca atgtgctcta gacctaattt tgaccaaaat gacatatcaa aaagtttaaa
    53101 aggaaaaaat ggagaatttg aaaatagagt tataaaagcg acatatccac cagggtctgt
    53161 atttaagatg gtagttttat tttctgcact tgaaaatggt gttatagatg agaactatac
    53221 ttacaactgt acaggtaaga ctaaagttgg gaatactaat gaaatactgc gatgtaataa
    53281 aagagatggt catggttttc aaaacttaag acaagctttt tctaattcat gcaatcctgc
    53341 atttttagat atagctatga aacttggaaa agaaaagata ctcaaatctg ctgaaaaatt
    53401 acatttattt gaaaaggttg atattggtct tgatgaagaa aaaattagag aagctccaaa
    53461 aaacatatca ataagaaatt tagctatagg tcaagaaaat atagaattta caccacttca
    53521 aataaatcaa atgactcaaa taatagctaa taatggaaca ttcaaaccac tatacttata
    53581 taaaagtctt gtggacaata atatgaatac aataaaaact tataagtctt caaaaaaaga
    53641 agaacttatt tctccttatg tgtgtacaca agttaaagaa tatatgaagt ctgtatctag
    53701 gactggtaca gctaaagacc taaaagatat tgaaggtggt tgtggagtta aaactggtac
    53761 agctcaaagt agtttaaata aaaaagctat agaccacgga tggataacag gattttatcc
    53821 tgaagaaaga cctaagtatg taataactgt gttagtagaa ggaactcaaa aaggaaataa
    53881 atctgcaaca cctatattta aggaaatatg tgaaagtata aaataaatta tgtactataa
    53941 aatttaagga tatatcgtaa ctttgatttt agatagaaaa ttttgtctaa tgatgtacga
    54001 tatatccttt taaaatatag tattaacttt cttgaacaag ctctttttct agttttttaa
    54061 gagctttttt ttcgattcta gatacatatg accttgatat atcgagtttt tgtgcaattt
    54121 ctctttgtgt cttatagcca taaggagtta acccatatct taactggaca atttctcttt
    54181 ctctaggagt aagaattttg tttagttgtt cgtaaagttt tccaacttga acttttagtt
    54241 caacttcatc aagaacatag ttagcctctg taccaagtat atctaaaaga cttatttcat
    54301 tacatactac tctttatatg ttattggtat tactggtctt catgtaattt caaatggacc
    54361 attaattcaa aagaatctag tttttgttct ttagacttat aataaactgc tttatcaatt
    54421 acagatttta gaagtttatt ttttccaagg gaatctgaat catggtaaag tgataacaca
    54481 gttttaatat cttctattat tttagaattg tcttttttaa cagtattcaa tctatttttt
    54541 actgtcttaa ttgaatcttt aatttcttca gttcttgaac ttatattgtt ggacctttca
    54601 ataaatgtat cttcatcata tattcctcgt tctagtaaat catataactt atttttttgc
    54661 ttgcctaatt cttttaattc agtttctaag ccctttaaga ctcgtttttg aaattctata
    54721 ttactatctt tattattttt atctaagtct atatcagatg cttttaattg atattttttt
    54781 acagtgtctt ccaaagattt taaaatggca gcttcaataa attcaaatcg agaactttta
    54841 ttgcaaccag gatgataaca aattatatag tcatggtctg catatggtct atatactaat
    54901 ggtctaccac aatttttgca aatcactatt cctgcaagtg gattttttat ttgattacca
    54961 tagctgacat gaccattttt tttgagtata ttttgagctt ttttccatgt tatttcactt
    55021 attaaaggtt catgctttcc ttttgcctca atccattcat ctttaggtct tagtttaact
    55081 gtttttattt tatttggatt tttagatttt ttgtagtcct ttttttgcca ctgaatgtag
    55141 ccacaatata ccttattttt tattatattt gtaattgcag aattgtacca attagcacct
    55201 gtagcagttt taatgcctaa gttatttagg tattttgata ttttactaca acccatatct
    55261 tcattaatat ataagtcaaa gatagtccta actacatcag catctttatt tggtacaagt
    55321 attctttctt ttctaccagt tgttacagca tcatatccaa atggagcaga ggttcctatg
    55381 aaattccctt cttcaactga ttttattcta cctctttgca tccttcttga tataagtttt
    55441 aattcttttc ttgccataaa tgcttcaaat tctgaatact cctcatcaaa ttcatctgtt
    55501 aggtcgtatg tttttctagg tgtgataatt ttggtcttac tttctttgaa tgtttctaat
    55561 atgatacctt ggtctttcat gtctccacga ccaagtctgt ctaagtccat tactaagact
    55621 gcatcatatt tattttcttc aacttctttt agaagttcaa gcattttggg tctatgtatt
    55681 atactttcac cagaaactaa ttcttctttt atctcaatta catctaagtt ttgttctttg
    55741 gctagtttta gtagggttga tttatgtcta cttaaagttt caaattcgcc ttgtttttct
    55801 gcctcttcat cagcacggga ttttcttaag tatatggcaa cactcataat aagtaatcac
    55861 actccttatg cactattata tagtaaatca atagagttta aaatatagaa aataagcatg
    55921 tcaaaagtta atttaacttt caacatactt attttctata atattaatgc cttataatcc
    55981 tttaatatca tttagattag tttttgcata ttcattatct gggttaagtt caagcgcctt
    56041 attgtaagat tctctagctt tattaaattc tgatttactc ataaaaataa cacctcttaa
    56101 attgtaagca tcactagcgt ctattttata agcttcatta tttttatcta gatattctaa
    56161 gtatttatca attgacttta aagcctcatc atatcgttgt aactctatat aaatatatga
    56221 ttgtgtataa tatgcaagtg gatttttatc atcaatttct atagcttttt tgatatcaat
    56281 agcagcttta ttataatcct tatcttcatc tttttctgtt tctgacctag cgattttgac
    56341 ataattagag ctaagagctc ttttaaagtc tttatgccat aataatttat attcattatc
    56401 ttcgattata aaatatctat caaaatttgc agtatcttct ttattttcag tgatatactt
    56461 tatagtatca gtttctttta ttttaacaag atttgtgtac tctttattag aatatttgta
    56521 tttctctact ttattttctt caccaagttt ataatctttt agggtttgaa gtttaaataa
    56581 agtatcttga tattcttcaa atttttctag actgacactt tccttactgt ctttacttaa
    56641 taaattatat gctacttcgt agtctttgat ttctatacta tcaaggtact ttttagctac
    56701 ttctgtagca ctttcttttg gagcacatcc aaccattgat atcataatta aaaatgatac
    56761 taatattgct gttatttttt tactgattat cataatataa tcccccttat attgtctctc
    56821 attaatttta ttttatcacc atattctacc aaagtcatta atttaagagt gatatttttc
    56881 attaatatta tattaagtaa taataacctt ttaaatttat ggtaacattt tcctgaaaag
    56941 tattatataa tttaggtgaa aatatttaag agaagtagat attatgaaaa ttaaaaaatt
    57001 attaacaata gggttatctt tatctatatt tattgctagt tgccctatat ctgcaaatgc
    57061 tcttgataaa attgaaccaa ttaaaggtgc agataaatac gaaactgctg gtataattgc
    57121 agacaaataa aactatacta cagctatatt aattaatgca gacagtacaa tgtctgatgg
    57181 attaagtgca agtggacttg caggagctat aaacgctcca attcttttga ctaagaaaaa
    57241 taacatacca aatgctacac ttaaaagatt agaaaaggca aaaaaagtat atataattgg
    57301 aggagaaaac tctattgata aatatactga aactgttcta aaaggcaaag gaatagagat
    57361 taaaagactt caaggtagtg atagaattaa gacaagctat aatgtcgcaa aagaaataaa
    57421 ctcaattaac aaagtaaata aagtcatatt gactaatgct tttaaaggag agcctgatgc
    57481 aatgagtgct gcacctgtag cagttagaga taaagcagct atagtattaa ctgatggaaa
    57541 aagtgtccat ttaatacaac tggtgtagaa agttatgtaa taggtggtac ttcatcaatg
    57601 aatgacaaaa tagttaagga tactaattca actagattag gtgggacaga tagatatgat
    57661 actaacaaaa agataataaa taaattttat agtggagtaa aagaatttta tatagcaagt
    57721 ggtactgatt tagtgtatgc tcttgttggt tctaccattg ctaaaaataa tgctattgtg
    57781 ttagttggta atgatagtaa taaaagtgta ttgaaaagta caactaaatt aactgctata
    57841 ggaaatttga gtgatagtat attacagcag tgtttaaatg taacaaaaaa tataggcgat
    57901 tctaatactg gaagagaata tacagtagaa gatgctgcta atgtagtgaa aaaactatta
    57961 ggtgtgaaga aattttatta cgaacctaat tattcagaag atgaagattt aacttacaat
    58021 ctatagagtg gagttcatgt acaaaagatt atcctcccaa cgattcgaag ggaggtgagt
    58081 tcatggataa ctttttgttt aatgttttag ctagtttaac agctagtgtg gtagtttact
    58141 taatcagtaa actattcaaa aaagcaaaaa gccactctcg cacaaagagt gacttacgag
    58201 ttgaatttaa atttatattt aaatccaaaa aataaactct gtttatgatg aactccactc
    58261 taacgcaaaa tagattgtag ttcttcttgc ttttattata ccacaaaaca ttataaataa
    58321 aactgttgtt tataaaaaaa tgcaaagtaa aattttttat aaacaatgat aaaaatttta
    58381 ttatagagct attaacttat gaaatatctg taaatgagca gatattttat aagttaatat
    58441 ttattgttta cataaaatat ttggtaaaaa taagtaagtt ttatatgtta taatagttgt
    58501 agcaagaata ataatctaaa gtggcaagca tgtcagctgg tgttatatca gtttatattt
    58561 atgataaaat aaaaatctcc caaacgccaa taagaatgat ttaaaaaaat aatattttca
    58621 cttaaaaata atcactcttt ataggagtaa attattttct tgattttatt atactacaaa
    58681 ttggtacaga tatttaaaaa taatatattc ataatattat aaatagtttt gcagtgagcg
    58741 atatttttga taaaataggg cttaacagtt gaaacataag gcattgaggg tatatgataa
    58801 atattatcat ttgcactact cgtggttcac tgcaaatttg agagaattgt atagatgtaa
    58861 gtgttggaaa tactcaattt attttggggt tttatattaa ctaagtggta tgtaaaggca
    58921 ataaaacctg ctattcttgg tagaaattca tttggtttta tattaactaa gtggtatgta
    58981 aagtttgacg tgttaagtga tatagataga agaatatgtg agttttatat taactaagtg
    59041 gtatgtaaag tttgtttctc tgtaacatga ttatcattca taattaaagt tttatattaa
    59101 ctaagtggta tgtaaagaga ggataaattg tttacatagt accagttggg tcttgtttta
    59161 tattaactaa gtggtatgta aagttgcact tgaatagttc tgaagaactt aagaactatg
    59221 gttttatatt aactaagtgg tatgtaaaga gctttacaat atcaaagaaa aattgaaaca
    59281 tctatgtttt atattaacta agtggtatgt aaagggaaaa ttatgggaaa agtttacaga
    59341 tatcatgaga gttttatatt aactaagtgg tatgtaaagt aaaaataaac ctcctaaaca
    59401 aatatttcac gcatagtttt atattaacta agtggtatgt aaagcttgta atcgctgaat
    59461 aaagatttct agcttcgtta ttgttttata ttaactaagt ggtatgtaaa ggactctgcc
    59521 atccctgtaa cgaacagcct tacagcaagt tttatattaa ctaagtggta tgtaaagact
    59581 tatctgtaac ccttatatgt tcaaattcat agccggtttt atattaacta agtggtatgt
    59641 aaagtaggat tgacacgtta cgaccgtgca acgttggact ggttttatat taactaagtg
    59701 gtatgtaaag gcaataatat tatcaatttt aatattttgt ttctcatgtt ttatattaac
    59761 taagtggtat gtaaagggtt ctggcaagtc acttcatgtt gctagagata tttgttttat
    59821 attaactaag tggtatgtaa aggttttttc ttcgtaactt tgagccttac atccacaaag
    59881 ttttatatta actaagtggt atgtaaagaa caaaaaagat acatctgtaa ctaattttta
    59941 tactgtttta tattaattaa gtggtatgta aagacagata taatgtcaaa ataaggttag
    60001 gacttttaaa agcatagcca tatcagtaaa aatttaatta tcatttaaaa aataaatttt
    60061 tatttaaaga atacacataa atatttccag actcaactct cttaaactat tttcatatct
    60121 tttagtagat acctttgttg ttttttaatc tttagcaact tgttcttgag tgaacctctt
    60181 attttttcta agttatttca agcttttcaa aaaattctct atttatattc atcacacata
    60241 taatacaatt aattcagttt taattgccaa tatttagttt ttctgtatct gataaaccaa
    60301 gaatataatc agtatataaa ccaaaaattt tagcaaatat tattaaccca tcttctcttg
    60361 ttggtctttc accagactct attctattca taacacttgt atttatattt gttttttcaa
    60421 gcaattcttt ttgagaacta ttcatatttt ccctaatata tttaatcctt tgatttacat
    60481 ccatacataa ttatctcgaa aagtttctgc tttggaaata ttgttattga aatatagaaa
    60541 ttaatgtgaa tatatgaaaa agatacacta tgattttata aaaaagaaga catacataaa
    60601 atttatactt acaaagtgtt gcaaaagagt taggttttaa aaatgcatca acttatttta
    60661 agtacaggaa agggtaatat tcaattagag ttgatatatt acctataaac tatctaagat
    60721 acttgattat gatatagaaa atttaattaa gaggatgata aggatgaata atttacaagt
    60781 aatataaaga aataataaga gaattttaaa tacatagcaa ctagcagata tatatgaaaa
    60841 tgatgtaaac aatatataaa ctagcttcaa tagaaataaa gagaatgtaa attatgtttt
    60901 attacaattc gagtatttga aagaatttaa gaactaacct actaatagtc agttgatttc
    60961 aaaacattct agtcaattat acatttggac cgaaagatgt gcaaatagac actgcaaaat
    61021 attagatatt gataaaactt gagaacaatt tgacaattta gagaaaatat atttcaagat
    61081 taagcgagag aaactaagct gtatagaaga tgtattaata gaaaatttaa tatttaagga
    61141 tacaaattaa tcaagcaata atatggcatt agaggtaaaa atagaagtta aaataataaa
    61201 agatgttgtt atattaaata taacacattg gaaaaaagaa acaagtctat taacagttaa
    61261 aattgctaga ggtcaaggtg atttaagtta tgtaaaatat tttactaatg agagctaaga
    61321 gcttcttaac aagagttttg gtgtgtctat tgaaataatg ttaacaatta ataaaagacg
    61381 aatggcagaa gaaggcgtat gtaagtttaa aagagataag ttaaacaaac tagatactat
    61441 ataagaggat aagaaactta tagaagaata tgttactttt gtaaaggatt tagctttaat
    61501 atatagaata taaagtgatt ttaagtaaaa attgtaacaa ttaaataagt ataagacaag
    61561 tttatgagtt catattttat aacaaggggt gatgttatga aaatactaca acaatttaaa
    61621 ccatacaaat atgataaaga aaaaaatata gaatatgatg aaaaaattat agctaatcaa
    61681 aacacagaag tttatatagt atatccacgt atatctgatg aagaaaatag gagaagatgg
    61741 gaagagttca atgaagtagc taaagaagta gctattaata tagctatgaa gaaagttgag
    61801 acagtagaaa gttaagagaa tagagaagtt gctaatactt agcactttct catagaagaa
    61861 aattggacaa ctatttttaa ataattagga ataaaaaagt taaatcatat ttgaaaaaag
    61921 gcgataaaat tgttgagtat tgttattagt aataagaatg tattaactat gtattaaaag
    61981 aaattataag tttttagtaa atatagtatt taaggatatt aaaactggta aaaaatatca
    62041 tttagactat taaataaaaa agtgctggtc aaagtaagca acacaataaa taaaaaaact
    62101 tatagaaatt ataagataaa aagatgagat ttgaaatggt tgaagttaat ataaaaatta
    62161 aaggtgaagt aagtgaaata attgactcaa ttagaggtat atttaatagc tttgatgaag
    62221 agttgatatt agatataaaa tcagagtaag aatataaagg tagatacgta gaaagttata
    62281 agtaattcaa gacttaaatt aatttctagt ttttttaatg caaaaattta ataaatttca
    62341 aaatataata attctgacat ctaaaaagag ctattaaaaa tgaataatat tgactattaa
    62401 gatatgaata gtgtacatat attaaattaa gatatccagt aggttaattt tatataatga
    62461 tggggctcaa attgaagagg cgatataaaa atgtattaat atatataaat ataagaacta
    62521 taataaatat taactgtaaa attaaattta tagagttata tttaacattt aatgaactga
    62581 aaaataagtt agcaaataat gaaaaaacaa cagtaataaa tgagaataac aaagcatttt
    62641 caggattagt ggatttttct aattgagctt taatcctagt ttgttcaatt tcgatatttc
    62701 taatattatt agtattatca atttatttgt aataattatc agtttttgcg aaatattttt
    62761 gataatttat agaactttat atattttcct tgaagtattt ttcgccctta ttgtttggtt
    62821 tccaatttta aaactcataa actcccaata aattcatgta taatatacct taatttataa
    62881 tatatttaag agtttatttc agtaattttt cgaacgattt tgatatattt catttatagt
    62941 tttcaaaaac ttatacaaaa aagagtgttt catttttcta aacactcttt ttataagaat
    63001 ttgaataata attttttaaa aattgataat atggttattt ctttttcaag tttatagttt
    63061 cctcatttcc attccaagat ataattagct tgtaactaga ttctctattt gaataaagac
    63121 ctggtctttg tatttttatt gctttttctt gacctgcttc taaaatttca ttttttacaa
    63181 ctacaactgc atcttcacca tctataaatt gataggaata attaaatttt tgttcaacta
    63241 catctttact atttttatat tttatttcta attttgagtg tccttcagta gttacaaatt
    63301 tcgctttcca attttctgat tctccaataa agacattttt atcatttttt atattaagta
    63361 tagctatcac agagattatt aaaactataa gaatacctat aaaaaatttt ttcctcatat
    63421 caatccctcc tttgaataat tataacatga gaatagttat tcaatactaa ttttaaaaat
    63481 attgtggatt tatattctat tacaatagaa tgtctgttat aacaaataaa aagtagggat
    63541 taatatgaat tataggttaa aaaaatctgt aacaactatt ttatctttac tcataatgac
    63601 aatactatta ccaacaaatg caagtgctag taaggtagaa gattcatcta tgaaagtaat
    63661 gtattcagag aaacaagtaa cagacatgga caaattatac gatatggcta aaaatggaat
    63721 tactgatgtt aaaagttcca atgaaaaagg tataatcaaa aatgcaagaa caggagagtc
    63781 catatacgtt gattctatat caacaactca attattagaa gttaaacaat ctaaaaattt
    63841 aacatcaaag atatattcta agacagtttt ttgatactaa tacaataaag cctcttagaa
    63901 aagcaagtat atcagaagat ggatgggata aaactggtgg tgtaaaagca tctggtacag
    63961 ttacatatac aatatcaaaa ggagatggag catttcaata tatttccgtt agtaaagttt
    64021 ctggaaactg gtctattgca gacggccaga ttcgtgtaac aaatagaagg gtgcatataa
    64081 atcaaactgg atttatttct aatgggctag gaattaagca gcaacaaaag aaagagccaa
    64141 catcaaattc tttctcatat aatgcacctt ctagttggaa accagtaggt agagattcag
    64201 ttcaatacaa ttgtgggttt gaatcaattg caacattagt aagaggagga agttcttggt
    64261 cattcaattt cattgtttaa ccttaaaata ttttaagtga attttattaa aattaaagat
    64321 atacatatac tttaaaatta gaaaatgtaa attaatttgt gtttttaaag ataattccta
    64381 agttatagca acaattgctt atttactatt aatatttctt aatatatgaa ttttatttta
    64441 acaaattata aagaatgtat attgggatgt ttttaaatat ttttggaaat tatatatatt
    64501 ataacaatta tagcaaattt ttagggtata atttttaaaa ttatattcta ctatcaaaag
    64561 atatgaataa atgattgtat atattatatc aatttttata tttcatagtc cattttttca
    64621 tcacattttc gcttgtcaag aataatatct ttaagagtga agcgtttgat gtaaatctgg
    64681 actttgtttt tgagacattt ttgaaatatt tattaaatct ttcgattata cttgtaatat
    64741 atattataga tattacttat tttagtaaaa ctaaaggatg atgctacaac attccagtta
    64801 tttcccaaaa ttttaatttc tgaagtatac tgtttacatt atttttaatc tattttatct
    64861 aaagtcgata aggcttctta ttcattaact gacctatata cagatttaaa attacttatt
    64921 aattcttttg agtccttgta tgaaacatat ttaaagtatt tcttatctaa tgtattaaac
    64981 atttttgtat tacagaatga ggataacttg tagctattgt ctctttgaaa taagtcatct
    65041 gtgtttactc ctaaaactac ttatatctgt agattttata agttctccat cttatataat
    65101 ttctttgatt aattgttttc ttaaattatt cataaaaaaa tctccgttac gatatttgat
    65161 tatatttaaa tcattgctat aaagaagact tttattaact ttgaaaaaca aattatttta
    65221 attgctggtg attagttgct accttctttt gatattaatt ctattagatt ttatatagtt
    65281 atatatctct ttctaactaa gtaatataat attactgtta tagt
//
LOCUS       DAMJVI010000007        63848 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.007, whole genome shotgun sequence.
ACCESSION   DAMJVI010000007 DAMJVI010000000
VERSION     DAMJVI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 63848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63848
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.007"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(184..2250)
                     /locus_tag="PC545_000569"
     CDS             complement(184..2250)
                     /locus_tag="PC545_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2455360.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLNNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLICSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKIIKCSQGDGGSVTYISPHDSEEEAL
                     YIAREIIDEIQKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     gene            complement(2327..5356)
                     /locus_tag="PC545_000570"
     CDS             complement(2327..5356)
                     /locus_tag="PC545_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2455361.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEATINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIKSISNDLIYLLRKNSVTGAFIILSDKNINDKSIDSKGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQDGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRLMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNNDMAFENVISNGYSLKEKFWDGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLDLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYIASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNAENVFCTKEFFSVFTTEIKFEEYGYMEK
                     NKFLKKLRGLDKYLQESYETENTYIYKFESKDKKLKWIRMNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKIAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSTNYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGV
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            complement(5409..6869)
                     /locus_tag="PC545_000571"
     CDS             complement(5409..6869)
                     /locus_tag="PC545_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDF2455362.1"
                     /translation="MNRCKFAKLIITSMIVLLLTGCSLKKEDNYKLDPENPTSIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGAEKGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELNEYIDEYVEEGRINSKDEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFKGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLDVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDSNKINELV
                     KSGTSKKDAVAQFNNDKNFKTWFESFKAELENAIKK"
     gene            complement(6961..7335)
                     /locus_tag="PC545_000572"
     CDS             complement(6961..7335)
                     /locus_tag="PC545_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455363.1"
                     /translation="MGNKNKNTKYKDKTRERYNKKVENLNKEKLNKINKQSKKVDKID
                     DIINKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVK
                     ALFSIFVIVCFLIAFKYVVNPV"
     gene            complement(7511..8251)
                     /locus_tag="PC545_000573"
     CDS             complement(7511..8251)
                     /locus_tag="PC545_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2455364.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDTLQNAKFKKIGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAIVKDNSRDKIIQIGKMLNEMNSYMRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSKDK"
     gene            complement(8272..9060)
                     /locus_tag="PC545_000574"
     CDS             complement(8272..9060)
                     /locus_tag="PC545_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HDF2455365.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLTFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISEND
                     ELDPFIRKGLELMVDGVDAVTIRNILESQIQITSKRHKEGIAIFDSAGGYAPTLGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEVSE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            complement(9148..9238)
                     /locus_tag="PC545_000575"
     tRNA            complement(9148..9238)
                     /locus_tag="PC545_000575"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9202..9204),aa:Ser,seq:gct)
     gene            9497..10261
                     /locus_tag="PC545_000576"
     CDS             9497..10261
                     /locus_tag="PC545_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2455366.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            complement(10542..11657)
                     /locus_tag="PC545_000577"
     CDS             complement(10542..11657)
                     /locus_tag="PC545_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2455367.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGIIGGLIA
                     GFNLSMVIINMIPVIILAVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(11698..13224)
                     /gene="glpK"
                     /locus_tag="PC545_000578"
     CDS             complement(11698..13224)
                     /gene="glpK"
                     /locus_tag="PC545_000578"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2455368.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDRELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTTQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            13517..14614
                     /locus_tag="PC545_000579"
     CDS             13517..14614
                     /locus_tag="PC545_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2455369.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLDILEKYIIENSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQNIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVNEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSKLEIKKVI"
     gene            complement(15067..15579)
                     /locus_tag="PC545_000580"
     CDS             complement(15067..15579)
                     /locus_tag="PC545_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455370.1"
                     /translation="MKVKKFKSGIVMALTVMAIGASLTNVSAMELNKSKEFETKYNEM
                     KMDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKMNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNAILRDEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            16132..17013
                     /locus_tag="PC545_000581"
     CDS             16132..17013
                     /locus_tag="PC545_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HDF2455371.1"
                     /translation="MKKRLIIMILSVVLVLSSILTIFAYSNIKYNNNKLIYSNMIDKK
                     TQNSVKEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPY
                     NLEKLQDNWVKKFPDYMDVNCRITAFRLFKDFINSNKKFTGDSIDLNVDLDTIMNNKD
                     AKFSIKDVEKFINFFSAIPAKDTDDTIKISEQIKNEWKKRKISFKNNKNISIINGFLR
                     YPETKNVFIGHTGICIKTKNGILFLEKYGVTSPYQVTKFKNKEDVKNYMFNRLKMSEG
                     EIELPDPIIMENDKLMK"
     gene            complement(17326..18105)
                     /gene="hisJ"
                     /locus_tag="PC545_000582"
     CDS             complement(17326..18105)
                     /gene="hisJ"
                     /locus_tag="PC545_000582"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HDF2455372.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDVGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKENL
                     NKYGKELEDSLLSVHIIKIDDDYYCVDYSVEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEELIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLILGSDSHCAEEINKYDIV"
     gene            complement(18651..19001)
                     /locus_tag="PC545_000583"
     CDS             complement(18651..19001)
                     /locus_tag="PC545_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HDF2455373.1"
                     /translation="MIFDKEIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(19017..19433)
                     /locus_tag="PC545_000584"
     CDS             complement(19017..19433)
                     /locus_tag="PC545_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HDF2455374.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(19452..19643)
                     /locus_tag="PC545_000585"
     CDS             complement(19452..19643)
                     /locus_tag="PC545_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HDF2455375.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(19794..20609)
                     /locus_tag="PC545_000586"
     CDS             complement(19794..20609)
                     /locus_tag="PC545_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDF2455376.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLVKELYEKYKNEEYNKKR"
     gene            complement(20656..21594)
                     /locus_tag="PC545_000587"
     CDS             complement(20656..21594)
                     /locus_tag="PC545_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2455377.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVIWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQIKKFFSVGVDKIGIAIDAANKELYEKIKGNNYDEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKENDVTISLFAFTPVRGTKMEKIN
                     QPSIESYRRVQLMSYMINKGYPKEYFKFKNGYLNSIKLDNDILKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(21591..22463)
                     /locus_tag="PC545_000588"
     CDS             complement(21591..22463)
                     /locus_tag="PC545_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2455378.1"
                     /translation="MNKNLSEKMEIAFDIKLKNFGNKIEFAYPNQTLALSTTSNQCSL
                     KCAHCNGHYLNNMVPIEEYEEKVQSRNITSFLLSGGCSYEGDVPINTHINTIKNLKEQ
                     GYRLNAHLGLMDKDSIVELCKYLDIVSFDLVFDDETIREVYKMKKSKEDYIEVYNTIQ
                     EHTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIVLIPTKGTEYENVEPPELE
                     GVADILCEARINLPDTEINLGCMRPRGVYRKELDQLSIMCGVNRIVLPSRSAKNKAIE
                     MNMTINECKECCVL"
     gene            complement(22488..24416)
                     /locus_tag="PC545_000589"
     CDS             complement(22488..24416)
                     /locus_tag="PC545_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HDF2455379.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTVFRKSDFKVTITYVQKQKKLTIINIEQGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(24528..24905)
                     /gene="gcvH"
                     /locus_tag="PC545_000590"
     CDS             complement(24528..24905)
                     /gene="gcvH"
                     /locus_tag="PC545_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDF2455380.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(24992..27118)
                     /gene="cdhC"
                     /locus_tag="PC545_000591"
     CDS             complement(24992..27118)
                     /gene="cdhC"
                     /locus_tag="PC545_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HDF2455381.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(27175..27981)
                     /locus_tag="PC545_000592"
     CDS             complement(27175..27981)
                     /locus_tag="PC545_000592"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2455382.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(28031..29398)
                     /locus_tag="PC545_000593"
     CDS             complement(28031..29398)
                     /locus_tag="PC545_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HDF2455383.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDAKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRTALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(29429..30373)
                     /locus_tag="PC545_000594"
     CDS             complement(29429..30373)
                     /locus_tag="PC545_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HDF2455384.1"
                     /translation="MAFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NSPKIGIQISDVYPESWTDSYKELYKDVANCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYAHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(30393..31163)
                     /locus_tag="PC545_000595"
     CDS             complement(30393..31163)
                     /locus_tag="PC545_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2455385.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(31219..32604)
                     /gene="lpdA"
                     /locus_tag="PC545_000596"
     CDS             complement(31219..32604)
                     /gene="lpdA"
                     /locus_tag="PC545_000596"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2455386.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVVEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(32648..33529)
                     /locus_tag="PC545_000597"
     CDS             complement(32648..33529)
                     /locus_tag="PC545_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDF2455387.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRAAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(33565..34239)
                     /locus_tag="PC545_000598"
     CDS             complement(33565..34239)
                     /locus_tag="PC545_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HDF2455388.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKILGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNSENDCAWIKIYERLEAIGQLDNLAEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(34276..35148)
                     /locus_tag="PC545_000599"
     CDS             complement(34276..35148)
                     /locus_tag="PC545_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HDF2455389.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQEEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSGVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLDKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(35240..35872)
                     /locus_tag="PC545_000600"
     CDS             complement(35240..35872)
                     /locus_tag="PC545_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HDF2455390.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVNKVKTEVDKLVEDGVKICDEVYAKVEKALGK"
     gene            complement(35977..37653)
                     /locus_tag="PC545_000601"
     CDS             complement(35977..37653)
                     /locus_tag="PC545_000601"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDF2455391.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDIFDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNNENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKELGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(37902..38681)
                     /locus_tag="PC545_000602"
     CDS             complement(37902..38681)
                     /locus_tag="PC545_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2455392.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDGYRVVAVDADPDANLAL
                     ALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPKVDDIPENFCKEYNGVR
                     LLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDMEAGIEHLGRGTAQGVDA
                     FIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKEDEEFIIQNLEDGESLGF
                     IYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
     gene            complement(38991..40910)
                     /gene="cooS"
                     /locus_tag="PC545_000603"
     CDS             complement(38991..40910)
                     /gene="cooS"
                     /locus_tag="PC545_000603"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2455393.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMESDAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            complement(41517..42428)
                     /locus_tag="PC545_000604"
     CDS             complement(41517..42428)
                     /locus_tag="PC545_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2455394.1"
                     /translation="MIKKLDDILEQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKAKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGENVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(42544..43122)
                     /locus_tag="PC545_000605"
     CDS             complement(42544..43122)
                     /locus_tag="PC545_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2455395.1"
                     /translation="MNINEFLTHINQRKPVSSKSEIHEFMVKLSNEAMKITSKLNSSY
                     HEPEEVRSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKNVAENTIVGGVPAKLLRVIDEIPE"
     gene            43348..44271
                     /locus_tag="PC545_000606"
     CDS             43348..44271
                     /locus_tag="PC545_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2455396.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIMELEEELG
                     VKLLVRGNRKITLTNEGRLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGSGETEA
                     MRLIARTAHHFHQKYPNVRYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYAQI
                     PATDVWGLLMRKDSALASQSTINPKDLCNIPLIISRQTLVDKAISKWLQGSNEKLNII
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSIMLDTSKLDTPKEK"
     gene            complement(44443..45585)
                     /locus_tag="PC545_000607"
     CDS             complement(44443..45585)
                     /locus_tag="PC545_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2455397.1"
                     /translation="MDFKIEKYLLKKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     SYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEKVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEVQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMDEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(45603..47357)
                     /locus_tag="PC545_000608"
     CDS             complement(45603..47357)
                     /locus_tag="PC545_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HDF2455398.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVNMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYEAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQDLDE
                     IPSPYENLNPKEYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLDRIKSDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREVENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            47493..47699
                     /locus_tag="PC545_000609"
     CDS             47493..47699
                     /locus_tag="PC545_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455399.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(47704..48627)
                     /locus_tag="PC545_000610"
     CDS             complement(47704..48627)
                     /locus_tag="PC545_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455400.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLTIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVIYFKKE
                     ELLFDYLLGSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKSEDYDEESILLNVLNVLYDVYMEDRTVENEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKSIDPRSLIRLEEGNTIVDPIFNQ
                     ITVMSKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(48881..50581)
                     /locus_tag="PC545_000611"
     CDS             complement(48881..50581)
                     /locus_tag="PC545_000611"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDF2455401.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFNK
                     LENGDEEAQELWQWFRNESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKN
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLKLLGMHAPERM
                     "
     gene            complement(50993..51406)
                     /locus_tag="PC545_000612"
     CDS             complement(50993..51406)
                     /locus_tag="PC545_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDF2455402.1"
                     /translation="MILVSACLIGINCKYSGDNNDNEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGIKVKSEKDL"
     gene            complement(51472..53850)
                     /locus_tag="PC545_000613"
     CDS             complement(51472..53850)
                     /locus_tag="PC545_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2455403.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKSEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTADSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKPGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIEKLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTRKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            54169..55620
                     /locus_tag="PC545_000614"
     CDS             54169..55620
                     /locus_tag="PC545_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDF2455404.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLVTVNEEDGMNGADALDPSL
                     IKGQYILNMDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLNVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKGSKLEKICSETYEKLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANVWDYVIEILKSMNQY"
     gene            complement(55824..58337)
                     /locus_tag="PC545_000615"
     CDS             complement(55824..58337)
                     /locus_tag="PC545_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2455405.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSCIFSFGINAIGNDNRTIRVA
                     YPIQKGLTEIDEQGNYFGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGMLYNEPMQKIYNYAGNNYGVANTVLQALYENTDIDNIMSSQDVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNDEQLEALKSGKADVLLNVSMNPLEGVRTV
                     AKFSSKPFYFITPKGSTNLTQKLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETDYIKETEKLKVGVLLNKPPFQNIDKDTGELRGISIDLLNHICDKTGLEFELVPVKS
                     QEDLENMTKKREIDIVAGMIYDYEYSREHNIVMSRPFLSTQYTMMINNKLNENSLDGK
                     RVALAKGINYYGQFEENIVWYDNLEECIEAVNKGYADYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYKAKSICFGIIKKDKQELLNIFNKAIISISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDKESENGNIENERDKIRKEYNKKFFDVIKSQE
                     NGTTEMYSIMLDDTFHWIRVTSKIIYNDRKIPMYVIGKICNIDSEKEEKNRLLDKAQR
                     DSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLLGDKVLIDV
                     AKVLDESFAKNDIVGRLGGDEFSVYINDIINKEELINKCNDVYNHIHLIKLPNGESLS
                     ISMGVVIAKPGQEYDELYQIADNALYDSKRQGRNRFKIV"
     gene            complement(58560..59381)
                     /locus_tag="PC545_000616"
     CDS             complement(58560..59381)
                     /locus_tag="PC545_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2455406.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVIEYNRESFELEPEKLLNFIKFRRSIRQYKDIEVEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIQGLYNLALDTDDNDPVRSIYKNT
                     FKKMYKRYKENGNDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVR"
     gene            complement(59899..60375)
                     /locus_tag="PC545_000617"
     CDS             complement(59899..60375)
                     /locus_tag="PC545_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HDF2455407.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYDNGKLKDYNVSFLD"
     gene            complement(60409..60987)
                     /locus_tag="PC545_000618"
     CDS             complement(60409..60987)
                     /locus_tag="PC545_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2455408.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRELAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(61091..63691)
                     /locus_tag="PC545_000619"
     CDS             complement(61091..63691)
                     /locus_tag="PC545_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDF2455409.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGLDRVVLARELNVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNIRAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDEERIKIKD
                     RKFKKNRLKYQPNRVNKQKKLDTVPKIRVKVKNLEQLEAVLNYNVDLIYYEDINTLSK
                     ALQMVSSISIDSNISIDSSISMDLDTSSSLDTPKELMGSNTNNIDTLSNTSKIDTLNK
                     NHYNTAVTNKKLIYSAPRIVRNKEYKQFEILDNIKGINKVQIGNWGSMEYFKKKDLNI
                     DCYLNAFNSESINHFKEEGASTVCLSQELNLNEIKQTLKYTDVEIEAVIYGYAPMMVT
                     EYCPMGVVVRDCKKDKRVAKCKESSYALRDFTDASYRITQDIFCRSTIYNSKVTCMLD
                     NLYELEEIGIDVFRIDFTVEDSEMVKKVIEAHIEVLSNNYKLGKKATDLYKKLDEIGT
                     VAGHYYKGVE"
BASE COUNT        19850 a        11955 c         7069 g        24974 t
ORIGIN      
        1 tgcttttctt taataatact aaataatatt ttccacattg ttgctttttc taaacaacta
       61 taaataatat attaaaccct tatttctttt tctaataagt taaataatac attttacaca
      121 tatctatttg tcttgcacat aataaataat acattatatt tctatctaaa tttagattct
      181 aaattaaata gtataaatta tattcttcaa taaacaagtt ttcaaatcca agattcgctc
      241 tctatcttta aactttattg taaacataat tccatctttt ttaattattt ctccttcatg
      301 aaatttttta tggtatacct tatcccctac accaatactg tcaatttctt ttttcgttgg
      361 agattttata tcattaataa ataatgattt atcaactttc ttaccatact tatttaaagt
      421 agagcttata cataactttt cttcagctct tgtaatagca acatacataa gtcttctttc
      481 ttcttctaat tgttcttctt ttttctcttc cttacaaata tcatatgatt tttcatgagg
      541 tattgttcct tcatttacac ctataatata aacatttcta aattctaatc cttttgcact
      601 atgcatagtt gtaaatatga caccctcagt ctgcttattc ttgttatttt ccgttaactc
      661 agtctttact ctatcaatat gctctaaaaa ctctgtaaca gtattaaaat tagttgctga
      721 actctcaagt tcatttaata tctctacaag tccattagtc tttatctttc tgttgctaca
      781 ataatctaaa acatatctat cataatcaag actagttcta atataagata ttgcattttt
      841 aggatttaaa gtatttaaat aacttaaatc tatttccaaa tcatttattg ttttaacttg
      901 tttaggatga agattacact tatttattaa tgctgttata aagtcttttt catccttaac
      961 catatttata ctgtctttgg atatatatct aaaaggcttg tttataattc tcaaccaatc
     1021 tttatttgat tttggattaa tacctattct taaataggct agtatatctt gacttgccca
     1081 atggtcatat attgtaatta ctgagtcttt tactacaaat ggaatcctca tatccataaa
     1141 tacatctact aatgctcttg actgtatatt agttctataa attactgcaa aatctgaata
     1201 ctctacataa tctttttgta tttcgtcaat tatttccctg gcaatatata aggcttcttc
     1261 ctctgaatca tgtggagaaa tataggtaac acttcctcca tccccctgac tacacttaat
     1321 gattttttca tatctatttt tatttttgtc gataagtcta tttgcagtat gcactatctc
     1381 acttttagac ctgtaattta tatctaatat tattttttta gtattattaa agtattgttc
     1441 aaattcaagc aaaaaatctg gtctagcacc tctaaatcca tatatacttt ggtcttcatc
     1501 tccaactgca aatatattgt ttaatgggct acaaattaac tttaaaactt caaactgtac
     1561 tttatttata tcttgaaatt catctatcaa tatatattta tacacatttc taaccatttc
     1621 tagtgcagac ttattgttca aaagtaaata ataagttctt ataagcatat catcaaaatc
     1681 tattttatta actttagatt tttgttcttc gtataagttg tatactttta aaaactcatc
     1741 ttttgttaat acttctgaat taaattcatt tttatccata agttcatttt ttacatagga
     1801 aatttcattt ataacttgtc ctatattctc atcatcatca gcattctcta tatttaaact
     1861 ttttaggatt gccttgattg tcattcgctt tgacttttca tcaaatatac tgtctaagtt
     1921 gtatctttca aagtatctta gaattttaaa aaatacagag tgaaaagtac cataggttac
     1981 cttattaagt cttatgtcat cacttaaatt caaagctcta tttttcattt ctattgaaga
     2041 tgccttggta aaacttattg ccaagattct tgttggtgct atgtttttgt ttacaaccat
     2101 attggctatt ctatatgtaa ttactctggt cttacctgaa ccaggccctg caagtatcat
     2161 gcaaggccca tctatatgct caactgcttc taattgattt tcatttaact ttcttatatc
     2221 tatatttgtt gtgattatta gctccaccac cttgatttat aattatatta ttttcttttt
     2281 aattataaac taaaagttca taaaaataaa tttaattttt cctctattaa accacatcat
     2341 tcctattttc atgacatatc ttgagttctt ttttaatttt gtcgcttata tcttcaggta
     2401 taaatgatgg tttactaata tagtagcctt gcatataatc tactccaaga ttaattagtg
     2461 tttccatttc accttttgtt tctacacctt ctgctatcac tttaatattt ctttcgttag
     2521 aatatgagat aagatttttt aatattttct gtctgttttc atctttatca atacctctta
     2581 caatagacat atcaatcttt acatagtctg gcattatata taatagtaca gcatctccgt
     2641 tgtaacctgt cccaaaatca tctaaagcaa tttctatatt ccaatggtct atatatgctt
     2701 tctttttttc aataaaatct tcattaatct tatcattctc tgttatttct agtactactc
     2761 tatttaaata ttcaccatat aaatcttcaa tttcctcaaa atctactttt gacaaattat
     2821 tattaggaat agaatttaag aacaatttgc aatcgccaaa tttttctttg tattttttaa
     2881 atgatttaag tgctttaaac catgtaatac gctcaatttg atataattta gactgtgaac
     2941 gagctaatct aatgacatct attggtgaac taaaagtttt tattttaggt ctcataagtg
     3001 cttcatatgc aaatacttta cctttacaat ctacaatagg ctgaaatgca tactcaacta
     3061 attgaccatc aattaacttg tttaattctt ctctattact caataagaat gaatccttat
     3121 tatatatatt aatatcaaat tcactaactt cacccttgat tgtattttta atttgataca
     3181 tagcaaaatc tgcgtatctc attaattcat catagttggt tgagtcatca gggtaccagg
     3241 aaattcctgc tgatgccctt attttaaatt ctgttccatc atgaagtttt aataaggtat
     3301 tattcatctg tttcttaaca gaattaataa tatctcttat actatctttg ctttcatatc
     3361 catatataaa tacataaaac tcatcacctg aaagtctaga tactatacca ttccatgata
     3421 taaacttctt aagtatgtct gcagcacacc taatatattc atcaccagca tcatgtccat
     3481 atgtatcatt aatatatttt agattatcta aatcccacat aataaatgct gctatcttaa
     3541 gttcatcttt attcttaaat ttttcttgta tctgtgaatg aaaagctctc ctatttaaaa
     3601 gattcgttaa aatatcaaaa tctctttcat attctatctt ccttttctca ataaattctt
     3661 ttgtgacatc aacaacaaca ccaagaacct ttagctcatc ttcaaagata ttcattctta
     3721 tccacttaag ttttttatct ttagattcaa acttatatat atatgtattt tctgtttcat
     3781 aactttcttg caaatattta tctagtcccc ttaatttttt caggaactta tttttttcca
     3841 tatatccata ttcttcaaat ttgatttcag tagtaaacac gctaaaaaat tcttttgtac
     3901 aaaaaacatt ctctgcgttt ttgtataact caaaagcccc aattggcata tctaccattt
     3961 caataatctg tgataatttt gacgaagaat ctgcaacatt tgcactgagc aactctatag
     4021 ctgaagataa ttcatctatt tcagatatat ttatcttatc aagatttact ggttttgatg
     4081 gattactgtt tctaactttg ttggctaaaa gaacaattgg ttttgtaaat aatcttgatg
     4141 caatgtaaat acctattaga cctattaata aagaaattgc tatagatatt ataactaatt
     4201 tctctaccct ttttgaaaat gacaaaagac ttttttcttc aataataccc aaaagagccc
     4261 aacgttcatt ttcaaatgga gtattggagt tatataaatc caagtattgt acactagcat
     4321 aaatgttgcc attaccacat tcattatctt tatttttaat cttatatata tttttatacg
     4381 attccttctg ctctaaatta gaatgtaaac catcccaaaa tttttctttt aatgagtatc
     4441 catttgaaat tacattttca aaggccatat cattatttct atctacacct aaaatataat
     4501 tacccttttt atttccattt atctctgagt atggaagtaa ttctcttaaa taatttacac
     4561 ttaaatctat tccaagtact ccatatacag ttccatcttc ataaataagg ggaactgtat
     4621 atgtcataag cctatcacct gcatttgtca ataaaaactt acttccccag tatcctaaat
     4681 tagaacttga tacatttgga ttatcaattg cagctctaaa tggtttataa aaaaaatcat
     4741 ctccatcttg ctcattttca cttttaaatg aaaacttagg ttcccaataa ctgtccatag
     4801 atattccaaa acttcttgct atatctgatg aacctctctc aattaacaaa tcagaattat
     4861 catttggatt aaacttaggg tctaaatccc ttatatacaa tcctgtccta cttatttcac
     4921 ctttactatc tattgactta tcgttaatat ttttgtcact taggattata aaagctcctg
     4981 taacagaatt ttttctcaaa agatatatta aatcatttga tatgcttttt ataatatctt
     5041 tgttaatttc ttcattgttg cctacatcct caattaaggc atttttttca tttaatactt
     5101 tttttacaga ggaattaatt gtagcttccg tttcacttgt atttgaccat ctttgtaaca
     5161 tttcattttg tatataattt ttacgattaa ttactctttc atttaagatg tcaaacgaat
     5221 tatcatttaa tttcttaata gttcctcccc ataaaattgt ggcacaaaat aaacttgttt
     5281 gaattagcat aacaataatt aggggaaata tcatcttttt cattattgaa ttgttttttc
     5341 ttagattgtt ttccataccc attccaccta tacctttgtg ctcgtcaaca cactaactgc
     5401 caattcatct atttttttat agcattttca agctcagcct taaaactttc aaaccaagtt
     5461 ttaaaatttt tatcattatt aaattgtgct actgcatcct tcttagaagt tccacttttt
     5521 actaattcat ttattttatt tgaatcttct ttagctttat caagcattga atattcaagt
     5581 atttgtctag ctttagttcc accatcaaat gctttattag tatataaatt acaactgtta
     5641 atctgttcaa tagatgaggc tattgaacct tcaactttat cagataaact caaattatct
     5701 ttcttcataa cttcttttat aacatccaaa tcattcgttt ttttctttac tggtaagtat
     5761 ccagaagcaa tagagaattt tatattaggt tctttatctg taaaccattt taaaaattct
     5821 actgatgcat attcttcttc tttagtagat tttgttacag ccattcctgc accttgttgt
     5881 acagcatatt ttccttctcc tttaaaacgt ggtgcttgaa aaacagatgt ttcaatatta
     5941 taactctcat tatcatttag cattactcta tctggaaagt atgctgctcc tgaactagaa
     6001 cctacaagag ctaatatatc acctgttttt gcatcatcag aacgaaactt accataagaa
     6061 gcaaaatatc catttatata tggtacatag aaattatccc atagtttttt cataactttt
     6121 tcatcaatat ttagtgtaac tttaccatct ttaacagaaa aaatttctac tcctagttgt
     6181 ttacttccta taataatata atttgctaaa gcatctcttc caaaaaatgc tttaccatca
     6241 ttaggctctt tcgttaaact atctgtccat tcataatatt tttttgatgt ctctactaat
     6301 ccttcaattg tttttatatc atttatattt gaatttgtag cttttgcaaa tttatcccaa
     6361 tcagttttat ttaacatcaa aatttcagtt gattttgcaa ttgggaaaag tttaaactca
     6421 tccttagaat ttattctgcc ttcttctaca tattcgtcta tgtattcatt taattcttct
     6481 ttacttatgt acttatccat atctactagt aaccctaatt tatccatttc atatgcagta
     6541 tctgagtatg ctgcaaatat atttggtaca tcttcagaac caactttttt atttgcagaa
     6601 tctaaaactt tctctgataa atcaactaca ttaccttgac tgaatgcttc aacaataatt
     6661 cctttttcag cacctacagt gtcattaaat tccttaacaa gcttatcaaa agccattttc
     6721 tgctgtccat tataataatg ccaaatttgt attgatgtcg gattttctgg gtctaactta
     6781 taattatctt ccttctttaa actacatcct gtaagtaata aaacaatcat tgatgtgata
     6841 attaattttg caaacttaca cctattcata aaatctactc cttcaaatta taaaatttat
     6901 aatagcatta aaactgtaac ttcactattt atattaatta tagaacgttt attgatatat
     6961 ttaaacagga tttacaacat atttaaaagc aattaagaaa catactatta caaatataga
     7021 aaataaagct tttacataat ttttatttct aaatgcacca aatgacacca atccttgttg
     7081 aatagctact gtaattacta aaagtatcat tattatatta agttgaacca gtttaaataa
     7141 gtaaagcaat gaaaacacta ctagaagtac acataataca atactagatt tattaataat
     7201 atcatcaatc ttatctactt tcttagattg cttattaatt ttatttaatt tttctttatt
     7261 taaattctca acttttttat tatacctttc tcttgtttta tctttatatt tagtattttt
     7321 atttttattt cccatttatt ttccatcctc tataaatttt ttaattgagc ttatacatta
     7381 tttaaatatt atatatttaa gataccatat attcctacaa aaaggtaact tactatgtat
     7441 ctatttttga ttttcgcata aaaaagccat ctcctttaga attaattctg tattgagaca
     7501 gctaatttaa ctatttatcc tttgaaacat tttctacatt atcatattta ctatcaacta
     7561 aaactatatc aactcttcta ttttttgacc taccttcttc agatgaattt tctgctattg
     7621 gtctaaactc accatatcct acagcagata atttatctgg tgctatacct gcattgtcaa
     7681 taagaagttg aacaacattg gttgctctca taactgataa atcccaattt gacttaaact
     7741 ctgaattttt tatagacata ttgtctgtat ggccttctac tctcatgtaa ctattcattt
     7801 catttaacat cttacctatt tgaataatct tatctctaga attatccttc acaatagctt
     7861 tccctgtatc aaatagtatt gtatctttaa ggcttattac taatcctcta tcttgaactc
     7921 ttaatgatac tgaattagcc ataccatttt cgtttaaata cttctgaatt tcttcaccaa
     7981 tttttttaaa cttagcattt tgtaaagtat ctaaatcatt acttcctggt tcaactactg
     8041 gttctatctt gctattacca ccttcaataa ctccagaaga accaccaaaa gcagaattta
     8101 gagattgaga taattgttta tatttctccg catcaacatt actcatagaa tacattatta
     8161 caaagaaaat cataagtaat gttatgaggt ctgaataagt taaaagccat cgctcattgt
     8221 tttcttcttt ttcatcttca tcatacaaca taaacattcc atccttttat attattcagc
     8281 tctaccacca atttcttcaa gttcaattag ttcttttgaa tttaaaaata tttttaattt
     8341 ttctgtcatt aaattagaat tatcaccctc aagtatggat aatattgctt ctatcaccaa
     8401 aagtctttca cttacttctt gttgatttat agccttaagt tttgatgcta ttggtaacca
     8461 tattaaattg gcagaaccaa caccatacaa agtagccaaa aatgcaacag aaatttgagg
     8521 tcctaatgca tctggattac ttgaaagatt acctaaaata tgtactaatc ccataactgt
     8581 acctattatc cccagtgtag gtgcatatcc acctgctgaa tcaaaaattg ctattccttc
     8641 tttatgtcgt tttgatgtta tctgtatctg tgattccaat atatttctta tagtaactgc
     8701 atccactcca tctaccataa gttcaagccc ttttctaata aaagggtcta attcatcatt
     8761 ctcacttatt tctttgtcaa tactaagaag cccctctttc ctagcctttg ttgcaatttc
     8821 tttgaaatag attatatttt ctatgttatt aggcttttta tttttaaaag ttaataacat
     8881 tatctttata gactttttaa acacagggaa tggagtcgaa gctccaacag ctcctatagt
     8941 tccaccaaac acaacaagtg ctgatgaaaa tacaaataat gctccaacat gtcccccatc
     9001 aataacaaaa gcaactacca aagatgctat ccccactatg aaaaaaatta aagttgtcat
     9061 ctattccctc ctttctttag ttatacatat ctaatcattt atgacattat aaaaagacta
     9121 tctttaataa aaatagcctt taataattgg cggagaagga gggattcgaa ccctcgcacc
     9181 ggttacccag cctaatccct tagcagggga tcctcttgag ccacttgagt acttctccat
     9241 atctctatac tagtaaatta tataataaaa tcatatttta gtcaattgtt ttataaaatt
     9301 tgagttattt tgaattatta aacatctatt tttacttttt tgcttaaatg taagttttaa
     9361 atatggtaaa attatcttgc ataacactta taatattatt tgataaatat acatacatta
     9421 acaaatatac ctaaactctg acttaattgt aagttatgcc aaaactaaaa taatactagt
     9481 ccaaggagga tattttatgt taccacagga aagatatgag aagattttaa gtattctcaa
     9541 atcagataat ataattaaaa taaatgacat agttcaaatg tttgatatat caatagaaac
     9601 tgcacgaaga gatttagccc atatggagag tatcggtctt ttaaaaagag tttatggtgg
     9661 tgctatacca gttatagcaa gtagcgtaga acttaactat gttaaaagaa aaaaaataaa
     9721 ctcaaacgat aaaaaacaga ttgctattaa atgtagtgag tttataaata caaatgatac
     9781 tatttttatg gatactggaa ctactgtact tgaagtttca aaaaatttga aagataaaaa
     9841 gaatctaact gtaataacca actcaatttt agttgcaaac gagcttatga atactaatat
     9901 aaaagtatat gtgcttgggg gctatcttag agaaactgaa ggttcttttt ctggtccgct
     9961 tacactgtca gctattgaac aatttaatgt tgataaagct attttaggcg ctggaggtat
    10021 cactcttgaa aatggaatct ctgactatca cttagaagaa gcacttgtta gaaaaaaaat
    10081 gattgaaagg tctagaaaat ctatagtagt tgtcgaacct cctaagtttg gtataaattc
    10141 atttgcatca gtagctccta tagaacatat agatactatt attacctctg ctagtataga
    10201 ccataatata ataatagagt tttctcaaaa aggaatacct ttagtcatag ctgataatta
    10261 gcttatatga ttgattttta gttccttgat gtaataaatt aaaattaagg agccaaataa
    10321 ttatttattt agctccattt atgtctaaac tatactttat attttaatta tgtaactact
    10381 ttcagttagc ttatcatttt tagttatttg attgccattt tatttaattt ataagtttta
    10441 gttattcgat tattttttaa ttgtctgtct ttttaattta tttttagtta tttaaactcc
    10501 tactttaaaa taatttaaat ttgacttaag tattgtttat tctacactgc atctttcttc
    10561 attttaatac tttgttcttc ttccaaggtt aaatcttttg tcataagtat agcaagtgca
    10621 attgccaata ctcctccaac caattttcca attacaacag gtgtaatcat ctctggaact
    10681 actcctgctg taaatcctaa atggtccccc aaagctgctg tagcacaaac cagccaagca
    10741 gtatttacta ctttcccctt tggactcata tctttcatca ttttatatac tggaatacta
    10801 ttggctaaag taaaaactaa tcctgctgca ctagtggcat ccatacctaa ttttccaccc
    10861 actattgtaa gtggtttatt aagaacttta actaataaat gtaatattgg aaaagtacct
    10921 aataacacga ttgcaatact tccaattact tgaatacctt ctcttatagg tgtcatgcct
    10981 tttataagta ctatacctgt tatttcttga aatgctgctg ctcccaaacc tatcgttatc
    11041 aatatagata taaattttcc aaaaactaat gacccattta tcataagcgt agaattaaat
    11101 tttaaaccta ctgccaatat tactgccaat attatcactg gtatcatatt tataattacc
    11161 attgataagt taaatcctgc tattaatcct cctataatac ctccaaaagg tattgttatc
    11221 aagcctatca gtaacccttt tgcaaagaat ggtctgtctt catattctat aagacctagt
    11281 cctactggaa ttgaaaatac taatgtacat ccaagcatgg atgacacaac caatcctgca
    11341 agcattcctg cctctttatt gtgtgcaagt tccatagcca atggataccc tcccatatca
    11401 ttcgctaaaa tagttgcaaa catagatggg tcagctccac agagattgaa gattggtatt
    11461 attataggac ctagtacatc tgaaattact ggagctaaac aaactatacc aaccattcct
    11521 aaagccaatg gacccattga attaaaccct tcttcaaact gctcgccaag tccaaattta
    11581 tttccaatta ttctatctaa cgctcctaaa agcactccca cagccattat gtataatata
    11641 attttatcaa agggcatttt taattcctcc tatacatgta aaatactcta ttgtaactta
    11701 attagcacac acctttttgg tatcgttttc ccaattcata gctcttttta ctgcttggtt
    11761 ccatccaagt aatatttctt ctcttttttg agtagtcata tttggaatat attttttgct
    11821 tattgtaaat tcacttctaa tttcatcaat actgttccag acaccacaag aaagacctgc
    11881 catatatcct actccaagta ctgtcgtctc aggattttct gcaatttcaa ttggtatacc
    11941 taatatgtcg gattgaaatt gcattgtaaa gttattttta gtaattccac catctactct
    12001 caacattttt atttttttcc ctgaggctaa gttcatagaa tcaacaacat ctttgacttg
    12061 aagtgcaaca gactcaagag tcgctcttac tatatgctct tttttagtac ttcctgttat
    12121 tccaaccatt gtacctctag catattggtc ccaataaggt gctgctatac ctgcaaatgc
    12181 tggtacaaaa tatacacctc cagtacttga tatggaattt gccatgtcat cagtttctga
    12241 ataatttttt ataactccaa tgccatctct aagccattgt attgcagcac ctgctatata
    12301 cactccacca tcataagcat atgtaacttt atctcctatt ttccaagcaa cagttgtcaa
    12361 aataccatta cctgctatat ttagttcctc acctatattc atatatagga aacatcctgt
    12421 accataagta tttttaacca ttcctggttc aaaacatcca ttgccaaata aagctgctgg
    12481 ttggtctacc attgaagcag taatttttat agaaacccca caaaatgcct ccttatctgt
    12541 agttccaaaa tcaccactcg taggcattat atctgctaaa atacttctag gtactcctat
    12601 aatgtcaagc aactctctat cccaatccat atctcttata ttaaaaagca ttgttcttgc
    12661 tgctgtagat acatcagtta aaaaagattt tcctcctgtc attttccata ttaaccaact
    12721 atctaatgtt cctgcaagta cttctccatt tttaattttt tcttttacac cttttacatt
    12781 atctattacc catttaattt ttgtacctga gaaatatggg tctataatta acccagtctt
    12841 atctattatc ttattttcta aacctggtat attctttaaa ttttccacat aatcataagt
    12901 tctcctgcac tgccacacaa tagcattgta aagtggttta cctgtatttt tatcccataa
    12961 cattactgtt tcaccttgat tatctatacc aattcctttt atatcacttt cattgattcc
    13021 attttcaaaa gcctgttgta atacattatt actcacagtt tttgtattat tccaaatttc
    13081 tatagggtca tgttcaacat atccagattg tggatatatt tgagtatgtt ccatataatc
    13141 gctaaaaaca aactctgatt tttcgtttat caatactgct cttgttcctg ttgttccttg
    13201 gtcaattgct aatacatatt tcactttaaa tgtccccctt tattttcaga attaataaat
    13261 tcttttttat tttttgaata tataatctat tcaagttatt tatattttat atcatcatgt
    13321 ggatttatgc aatattcatt acaatatttt ttgttatttt taattatagt gattaaaaaa
    13381 gtatttttat tgaaatatta ttgcaatatt tattatttta ttctataatc tagttaaaat
    13441 aacacataat attttaatat ttacacataa tcagtcattc aaaaatttaa atttaacttt
    13501 ttaaggaggc tttacaatga aaaaaacata tctattcagt cctggaccag taatggttac
    13561 agataaagtc agaaattcac ttcttcacta tgatatatgc catagaggaa atgagtttat
    13621 ggatttattc aaagatacac aacaaaaaat caataaacta tataatgcaa cttctaatta
    13681 ctactcatta attgtatctg gctctggaac atctgtaaat gaatgtgtac tatcttctat
    13741 ctttgacaaa gaagatgcag ttctgctagt tagtaatggt gtttttggag aaaggctaga
    13801 agaaatatta tctacttata atgtaaaaac atataaacct aattttgaat gggcagaata
    13861 cccggaccta gacattttag aaaaatacat aattgaaaat tctgatataa aagtaattgc
    13921 tatggtgttc catgaaacta gttcaggaat gataaatcca gtccctgaac taggaaaact
    13981 tgctaagaag tataacaaaa tattttttgt agatgcaata agtgcctcag caggtgaata
    14041 tattgatgtg gaagagttta atatagatat aataacaggt gttggtggca aagctcttgg
    14101 agcatttcca ggttctgcat acctttgtgc taaagagagt attttacaaa atattaaaga
    14161 aaatcaatgt aagaatgtgt atcttaatct ttataaacac tataaagttg ctaaatcatc
    14221 ttctcaaaca ccaaatacac ctaatgttac tttaatattt gcccttaatg aagctttaac
    14281 tgagtttttg gaagatgaca gtaaaataga aagatacaaa gaatgttctg ctattttaag
    14341 gaatggtatg gaagaattgg gtctaacatt tttacttcca gataagtata tgtctaatac
    14401 agtaacatct gtatttctac ctaaagaaat caatgtaaat gagtttatat tagggctaga
    14461 aagggaaagt ggctatgttg tctatccagg aaaaggtaaa ttcctagatg ctaatatatt
    14521 ccaagtagca aatatgggag aaatatatcc agaagattgt tataaattct tagatatctt
    14581 aaaatcaaaa ttggagataa aaaaagttat ataacagttt gtttgtaaaa tcctacaata
    14641 tatcagtata aattaaatag actgattttt ttaaattttc ctataataaa ttatatttta
    14701 tatttttgta taagggtgaa ctataatatg taaagttcac ccctatttca aatctttttc
    14761 cttcaatata atctttttct tttaatttat tttatatttt tgacataatt attcttacat
    14821 ttaattgttt tttatttttg acgcaatcgt tttttattca gtcattttat attcttgata
    14881 caatcattct tacacttaat tattttttat ttttgacaca atcgtttttt attcagtcat
    14941 ttaatatttt caacacaatc attcttacat tctattattg tttgttttga tacaactgtt
    15001 tttattcagt cattttatat tctcaacaca attacctttt cattcaatca aagtaatctt
    15061 taagcattat ttttcaagct catcaaagta attatatacg ataacctcac ctgtctcttt
    15121 atctgtagtc tttataatgc ccttagctcc atttacctct tcttttgtaa attcatcatc
    15181 taacacaact ttgaatttat cttcctttcc ataaccttgt tgaaattcat ctctcaaaat
    15241 tgcatttttt tctgcttttg gcttcaactc aaattcaatt ccagatttca tttcatcaaa
    15301 ataatttaat actacaacct cattattcat tttattagtt gtctttataa tccctttagc
    15361 tccatttact tcttcttttg taaattcatc atccaatgtt atattaaatt tgtcttcttt
    15421 tccatattcc tcttgaaaat ccatcttcat ttcattgtat ttagtttcaa actctttact
    15481 cttgtttaat tccatagctg atacatttgt caatgatgct ccaattgcca ttactgttaa
    15541 tgccattact ataccacttt taaatttttt tactttcata tcttacctcc taaattaaat
    15601 gttctcaatg aaattatata aagaaaatcc taagttttac agtttcattc ttgtaactaa
    15661 aattactatt atgtaaccaa actttttgct tcaactattc cttaaaagtt gcttttttga
    15721 ggaatagttg aagttaaata attagatttt atctatattt atagatttaa aatcaaattt
    15781 atccatataa ataaatactt atttttgcaa aataaaaatt tttaattact ccaatccttc
    15841 ttatttactt aaaatcctat gataagaaat ttaaaagtct ttaccttctt taacaataat
    15901 ttatttactt ttaacataca aaatatagag agtatatcaa aatcatttta ctgaatattc
    15961 taaaaataag taccccctat tagtatatat atttacatat taaaaaaaag ttttaaataa
    16021 agttatattt tttacaacag ttttgtaatt aaaaacatat tgaaggtaaa tttaaaggta
    16081 ctatatacta taaatatgtc gacacaataa atttaaaaag gagaattaaa tatgaaaaaa
    16141 agattaataa taatgatatt gtctgttgtc ttagtactat caagtatact tacgatattt
    16201 gcatattcaa atattaaata taataataat aaattgatat attctaatat gatagataaa
    16261 aaaactcaaa atagtgtaaa ggaaatccta aaagaaaata aaattaatga aaaagacata
    16321 gatacattta ttaaagcagt caacaattat aataaattgc aagttaaaat acttcaaaat
    16381 aatataaata tatctaaatc tggatattct tcaatcaatg caaaacaagt tccatataac
    16441 ctagaaaaat tgcaagataa ttgggtgaaa aaattccctg attatatgga tgtaaattgt
    16501 agaataactg cttttagact ttttaaagat tttataaatt caaataagaa atttactgga
    16561 gactctattg atttgaatgt cgacttggac actataatga ataataaaga tgctaaattt
    16621 agcatcaaag atgtagaaaa atttatcaac ttcttttctg caataccagc taaagataca
    16681 gatgatacaa tcaaaatttc agaacaaata aaaaatgaat ggaaaaaaag aaaaatatct
    16741 tttaaaaata ataaaaatat atctattata aatggatttc ttcgttatcc agaaactaaa
    16801 aacgtattta taggtcatac aggcatatgt attaaaacta aaaatggcat tctattttta
    16861 gaaaaatacg gtgttacatc accttatcaa gttactaaat ttaaaaataa agaagatgta
    16921 aaaaattata tgtttaatag attgaagatg tcagaaggtg aaattgaatt acctgaccct
    16981 ataataatgg aaaacgataa acttatgaaa taataacttt aaacgcaaaa taatatttat
    17041 aattactaag tcaatataat agagaaggtc tatagatttg tgtaaataca agcttatcta
    17101 caatcttata taagaatatt tatataaata agatagatta attctaagtc ctattattct
    17161 attgaatttt attattattt tatttagatt ctattgttat tttacttaga acttattatt
    17221 attttgttta agttctatta ctattttcaa tcatataaat ttacaaaaga ctgtacataa
    17281 atttatttta tacaaactta tctacagtct tttgtgtgaa tatatctaaa caatatcgta
    17341 tttatttatt tcctctgcac agtgagaatc agaacctaaa attaatggaa tatcgtattt
    17401 ttttaccaac tctaataagt atccatctat ataaggctct ttgcagtact cttttctatt
    17461 tccagaaaca ttataatcaa gttcataacc tttttcttta attaacttta ttaactcttc
    17521 aagaattgta tttcctttat aatcgtatgg aaatacctga ttaaactttc taactaaatt
    17581 taaatgacct attctttttg gtttatgtat acctaaatct gaatttacag cttttatcaa
    17641 tgtttcataa tacttattat aaacagcttc aatacttcct aatttgtcga ttaaattttg
    17701 aaactcctct acactgtaat ctacacaata ataatcatca tcaatcttaa ttatatgtac
    17761 tgataaaaga gaatcctcta attcctttcc atatttattt aaattctcct ttatcccttc
    17821 ttcgtatcct tctataaaat ccacttccga cccaacattt atcttgattt taccctcata
    17881 cttttctttc aacttcttga catcattaaa atactttggt aaatcttcaa tactcataca
    17941 actatcattt tcaggagcag ggtctctaaa tccatttgca tacggaaaat gttctgtaaa
    18001 tgtcatttct gttattccaa catccattgc tctttgtaca tatttttcaa aatcatcctt
    18061 acttccatga ggacaataag gtgaatgtat atgaccatct ttcataaata attcactcct
    18121 tttttaaaga aaaaatctaa tataaatgca taaatctttt aacttcaaaa taatattttt
    18181 aatcatgcta atttttatta tatctaatat tagagtaatt ttaaactgcc ttttaagtat
    18241 cttatcatta gtactatttt caacttaaca ttaatattat tttttaactt aacattaata
    18301 ctatctttaa atagtaatac tcaatatata attacttaac ataaaataac tcaaattgct
    18361 tgatgaggct tttgcagtat ttaaagaata tataattatt taacattaaa taatccaatc
    18421 ttgtaataaa atttactcat taaagtatat tacagaaaat acctcaaaat aaacttttta
    18481 gttcatgata tactcttttt tgcatgaaat tagatatatt tttatcattt caacaatgaa
    18541 agggaggctt atatccatct ttagacaacc ccttagataa aatttttaat aattaaacat
    18601 cacaaaatat aaaaatcgct tatatcaaac gtataagcga ttaattatat ttacatatgt
    18661 tttttagcct ctaacaacat tttattagct tcttccatat actctatagc cttttcaata
    18721 ctctttgctg tttcttcttt tccaatagct ctagcctttt caacccactc tctaaatcct
    18781 tcttcatgag tttcattgtg atttatccaa tgtactaaaa gtatttttaa agttttttca
    18841 tccttagact ctgatgattc ctcatgacta tgttcgtggt cgtggtcgtg agcatggtca
    18901 tgtggatgtt catgacatat atctccatga gtatgttcgt ggcagtggtc atggtcatga
    18961 tgtccatgat gatatcttac aatctcttta tcaaaaatca ttagttattc cccctgttat
    19021 tttatattaa actctttttt tataaggttt acagaatctt ttacaagtag tgctaatggt
    19081 ttaccttcta atccaactaa ctccacattt tctggcataa taggtaaaag tatcttaagt
    19141 gcctcactgt ctgcaatagc ttcacttata ttacaagtaa cttctcccat catagaatta
    19201 ggtattatta cagaaatagg tgcaactatt atatttgctt ttttagctga tactactata
    19261 gcattttccc ctgtagctcc tttattagca tgagctttca tcatagaact agttgctata
    19321 gaattagtcc ctagtgcata aatttctaca tatgtaggta actcttctct caaagaagaa
    19381 actatctggg ctccaatacc tccacccatt ccatctatca ctgctattat catatgaaat
    19441 tcccctatta attaattttc ttgtattatt atcttatggt ccatcaatct tatttcttta
    19501 attatcccat ctattatctt ttgctctcct aataaatccg ttaaataaat ctttccatca
    19561 caagggtcta tattaaccac attttccata accttttcta actcattatt acttttacaa
    19621 atgaaagctg atgattcaca catataaatc tcctcctaat atgattttta ttaaaaatca
    19681 aaaaaagatt cccttaggta tttcaataca ccaaagaaac cttcatgatt tcagtttttg
    19741 tatattatta taccttaagt atatcacttt aatttatgta aagtcaatta aagttaacgc
    19801 tttttattat actcttcatt cttgtatttt tcatatagtt ctttaactaa ttgcttctca
    19861 taatcagtca aatcgccctc cacaaactca acattaaatt gttcagctaa tccctttact
    19921 atgtttttct gtagaatatc tatgtctatt tttctgccaa tttcattttc tattccactt
    19981 gcttttttag ctgtaaattt catagctctt tctttcaatt ctggtgtctt agtttttatt
    20041 aatttaaaca atttttctac atcaaaatcc agtattattg aaccatgttg taataaaaca
    20101 ccatcttttc tgctttgagc acttccaatt accttcttat tattaatagt aacttcatat
    20161 gatgcatgtg cattaaaaca tgcagctgat aaattttccc tacttattct ctcgcctcta
    20221 tttaaattat ctgtttctat accacttaga ttcaaccctt taactagacc ttcacttata
    20281 tatctataag ataggttgat gctcttatcg attaaaggat tatcttctcc tataattata
    20341 ctataagtta gctcattatc atgtaataca gctcttccac ctgtaattct tctagtatag
    20401 tctatattca tacatctaca cttatctaaa tctatctcat cttccaattt ctgaaaatat
    20461 cctatactca aacaagctgg ttcccatgta taaaatctaa gagttggctt attatgtccc
    20521 ttcttatatg cagtaaatat agcttcgtca attgccatat tcattgctcc ttcatatgat
    20581 ttattatgta taactctcca ctgattcatg attattacgc ccccattttt agtataaata
    20641 tatcctagaa atcctctata gatttatttc tcttattgct aaatctatct cactttgatt
    20701 taaaggtctt gaatagttgt atatagtact tcctggtctt tcattatagt atggtctatt
    20761 acaatcctta caacctctta tttcaaaagg atagcctttg tttatgtctt ttaaaatatc
    20821 attgtcaagc tttatactat ttaagtatcc atttttaaac ttaaaatact cttttggata
    20881 acctttattt atcatataag acattaactg tacccttcta taactttcta tgcttggttg
    20941 atttatcttt tccattttag ttcctctaac tggagtaaat gcaaataaac taatcgttac
    21001 atcattttcc tttaaataag tatataaatt gtatatatct tcatgacttt cacccatacc
    21061 aactattata tgtgtactta ttttattggg atatgatttt gacatttcag ttataaattt
    21121 taacttttca tcatagttat ttccttttat tttttcatat agttctttat ttgctgcatc
    21181 tattgctatt cctattttat caactcctac tgaaaaaaac ttctttatct gttcatcatt
    21241 ttctaatttt gcagatagtg aaattggcat gtctatctta ccacttatat agtttataaa
    21301 gtctaaaaca gagttatgtg cttcttcact tgccattgat tgaatacata ttctctttat
    21361 attttttcct ttataagctt ttaaagcatc taatatttct tcttttgaaa actctggcca
    21421 aattactctt gataatttat cttttctagt actactttct attgattgag aacaaaaact
    21481 gcatttattt atgcattttt ctccaatcat tatatatgct gtggtaggag gtatatcgct
    21541 ttttttgttt aatatcccaa gttctattgc tgtcccaact gatagtctaa tcataaaacg
    21601 caacactcct tacactcatt tattgtcata ttcatttcta tagccttatt ctttgcagac
    21661 cttgatggta atactattct attgactcca cacattatag ataattggtc tagctctttt
    21721 ctatacactc ctcttggtct catacaacct agattaattt cagtatctgg taaattaatt
    21781 ctagcttcac ataaaatatc tgctacccct tctaactcag gaggttctac attttcatac
    21841 tcagtccctt ttgttggaat aaggactata aatgtaagct tattaggagg atttttttgt
    21901 aggtattcta taatttcata ttcacccttt atttgtcctc cttttaaacc aatacatata
    21961 tgtggtgcta cttctgtatg ttcttgtatt gtattataga cttcaatata atcttcttta
    22021 ctctttttca tcttataaac ttctcttatt gtctcatcat caaacactaa atcaaaagaa
    22081 actatatcta aatacttaca caactctaca atgctatctt tatccataag acctaaatga
    22141 gcattcaatc tatatccttg ctcttttaag tttttaatgg tattaatatg agtatttata
    22201 gggacatctc cctcataact acaaccgcca ctcaatagaa agctagttat attccttgat
    22261 tgtacttttt cctcatattc ttctattggc accatattgt ttaagtagtg accgttgcaa
    22321 tgggcacatt tcaaactaca ttgattgctt gtagtactaa gagctagtgt ctggttagga
    22381 taagcaaact ctattttatt tccaaaattc tttaatttaa tatcaaatgc tatttccatt
    22441 ttttcagata gattcttatt catagtttat tccttcttaa gtacatttta ctcttctagt
    22501 aatttctttg ctgttgggaa ctgctctata ttcgtatgtg gtaagaaaca tgcagaaata
    22561 aattcatcca tataacttgg atatacacta agttcaacat aagttatttg attacctatt
    22621 tcttcaagtt tattttttgc atctttactt ataagagcta agtaagaacc aacaagtgaa
    22681 ctattcccta tataagtaaa tttttctcta tcaatatcag gaagtaaccc tataattatt
    22741 gagttttcta tgtctaggtt gtttcctata ccacctgcta tataaaccct atctaatata
    22801 ctaaaatcca tacctaaact atctacaaga gtgtatgcgc ctgaatatat agctccttta
    22861 gctctgatga aattatctat atcaacttca ttgactacta tatcattttc taaatcaaat
    22921 tcttctttaa atgctaatac atactctcct atctcatgct cattaaatct aactctctta
    22981 ttatctaaat ccctgtatat tttacctctt ctatctatta cccctttagt aatcatttga
    23041 cagattaaat caattatacc agaaccacat ataccaactg gtgcacattc atcaataatc
    23101 tttaatgttg gctctaaagt ttctttatca ataattactt tctctattgc tcctgctgat
    23161 gctctcattc cacagcttat ccctccacct tcaaaagcag gtcctgctga acatgcacag
    23221 ctcatcatat aatctttatt tccaaataca atctctccat tagttcctaa atctataaat
    23281 aaaacatttt cttcacttga ccatatccct gctgataata cacctgctgt tatatctcct
    23341 cctacataac ttgcaacaga tggagctaag tacacatatg cactgtcatt tacaaaaaga
    23401 ccaacatttt cacccataag ttttggagat tttaaaaatg gtggtatgta aggttcttgt
    23461 cttaagaagt ctgtatacac acccaagaat aaacttgtca tagttgtatt cccagcagcc
    23521 actaatgtaa ctacatcttc cttatctatt ccatttcttt catatattgt ttttaatagt
    23581 ggatttatag tttcctcaac tatagcttta tgaagtgtct ctaatccatt tttcttagtt
    23641 gaatatataa ttctatttat aacatctgct ccatatttta tctgtgcatt tccagaagat
    23701 gctttatcta ctacctcttt tgagtatagg tctactaaac aaacaactac tgaagttgta
    23761 ccaatatcta ttgcaactcc gtataaggaa ttttctttat ttccttgttc tatatttatg
    23821 attgtaagtt tcttttgttt ttgaacatat gttattgtaa ccttaaaatc actctttcta
    23881 aatacagttg gcatttttct aagtatatcc aatctaaaat ctatttcatt atatcctagg
    23941 ttatttctaa catatctttc caatctatct acatcactta tattatcatc taaatttggt
    24001 tcttccattt ctatgtattt cttttttata tttgttttaa attgaaggtt atgttcttca
    24061 attatttttt tagctctttc aaaaatttcc ctatcctctt ttttattact accttctatc
    24121 ttcataccat gcatagaaga tgatacttta gaaggaactt caatttctat atctgatatt
    24181 acctttgtac aacaagctag tatataacct tgctcccact catcatctgt tatatgtctt
    24241 gttttttctg tatcaacttt tccattcaat aattttactt tacacttccc acaagacacg
    24301 tttccattac aaggagcatc tatgaatata tctgcatttc ttgcaacctc tagtaatata
    24361 tccccctcat tacaataaac ctctttattg tttggtgtaa aacttacttt tatcatgtta
    24421 tccctcccaa tttgttctat atatgataaa aggaaagtat agctaaattt gcgtgcactc
    24481 aaattttact atactttccc caattataat aatatcttaa tctttaatta ttctaatcca
    24541 gcctcgtatt ctttatcact taataacgta tcaagttcag ccacatcagc tagttttact
    24601 tttaatatcc agttttcata tgggtcttca tttatacatt ctggctcatc ttctaatttt
    24661 tcattaactt ccaatacctc accattaaca ggagatatta aatcagaagc tactttagaa
    24721 gactcaacta ctccaaaatc tactccctta gttacagtat cttctacttc tggtgtttca
    24781 acaaacaata tttctcctaa ttgatcttga gcatagtctg ttatacctat atatacaaca
    24841 tctccatcaa ttacttttac ccactcatga tctttagagt attttaattc tggtaataat
    24901 ttcatttcga tagaccccca atattatatt attgattaaa ttacttaaat catattaata
    24961 ttatatcata ttattttaaa ttttccatca attacattac actttccata gctaatgctg
    25021 gatgaccttt ttcttctaag aaagctaata ctccttcaga atcgtcagct atagtttcat
    25081 cacaaaccat atcacagaag ttttctattc cagtcatttc tttaactgta gcatttaatt
    25141 tatcagctac ataatctttt aattcttttg gcatccaaac tattctttct ggtccacctt
    25201 ctgcataagc gaatttttta gaagatatga aatgtcttcc gtgtcccata aatcctggag
    25261 tttgaactcc tccacctgtc atagaagcaa gttctccgaa agtcatacca actggagtaa
    25321 cacttgcaaa ttctctgtta actacaacaa atccatttgc ttctggcatt ataccacaga
    25381 tacattcgaa gcatccacaa gaagtcattg ggtcttctag tatagagtat aatgtaacag
    25441 attcaactgc accttgagat atttggttta cagtttcatt tactgaattc catacacctg
    25501 ttctatcatc taagcactcg cctttttcta ttggttgaca aggacctgtt gggtctaatt
    25561 ctttagtagc cttagcatct aaccagctaa ctgctccaca aagaccaagt ctttcaggag
    25621 taacaacaca aacgtgagct ggtgcaaatg attgacataa gttacaagaa tagaaagtat
    25681 caacactttc atcaactaat gaagctaatc tttcatctct agcattgtac ttagctattg
    25741 cttctccttt taattcagaa actttttcag catctgtaat tatagttatt tcacacttat
    25801 caactactga ttcaaattca tctaacatct tagcatataa aacttctcct atatgctcaa
    25861 gtctaaatcc tttatcaaat gcatctttag agattcttac ccaagccata tctctttgtc
    25921 caacgtgcat tacaccttct atatagttta agaagtagtg gaaacgtctt tcaagaactg
    25981 gctcgaaatc ttcttgcata tttttaccag ctattttaac tattacagca agtggtaatc
    26041 ttaatacacc atctgcatca acttcatcta tatttggtcc tattatttct atcttatggt
    26101 cttcaacttc tgttgcttct ttcttaacaa caagctccca agcttcagtt ctgtttccac
    26161 caaactcagc aaacatatcg ctctttctaa ttctctcacc ttcaaatgct gctgcaaatg
    26221 aaactgggat aggtatttca gtaactttta tttttattcc tctagcttct aatgaagtag
    26281 ctactatctt atcgtaatct ttttgagtta ataggttcat tggaacttca agaactgttt
    26341 ggtcagttat aactgggaat cctaaagcta ttgctccagc acctgcagat acaactaatt
    26401 cacttaatgg tccaaatgca ttaacaaatg caggaactct attagctgtg tactctaata
    26461 atccgtttaa atcaccagga gttaatccac cgaatattaa tgctgctctt actgcaacag
    26521 atacaacgtg tataacagaa gttacatcat atcctagagg tataactctt agctcaagac
    26581 ccatttttac tccagcttct atagcttggt ctataacttt accaactaag aaagttagta
    26641 aacctttaga ttggtaatct tttataactt ttgctgctga ttctgcatct ggacactcac
    26701 caagaacaac tgcaacacca ggtatatctc ctgtaactaa tggtacacca agtgatctga
    26761 ttatagggtc agttatatga cctgcacatg gctcactata tggagcatcc atagttgaat
    26821 attttaatgc ttcaattact tctgctgcta aagctgtagc taaaccagca ttaaaagcat
    26881 gttctagtaa atgagttcta tttattaaag attttactac ttctaaagca ccttctaagt
    26941 cccctaaagt gttcatcttt tgtcctgttg cagcatatat acaaggtaat gaatatgctg
    27001 tatcagggaa agcaacttta tgctcttttc cattcttttc tatagcttca gctaacatag
    27061 cttgagccgc acctaaagct tgttctgacc ctgtaaagat tatattatat agattcattc
    27121 tatttccccc tttaatattt acacctattt tttattaact ataccctaaa aaccctagaa
    27181 agcaaatcca ccttcattta actctaagaa agagtctgca tataaagatg ccccgttgat
    27241 tatgtcacaa gattttacag ttttcattaa ttctgggtca catggattca ttatagctga
    27301 actaaatcca ttagccattg ccattgctaa gaatgcacta tctaaaacag gtctaacatg
    27361 cttaggacat ccattagata cgttagataa tcctcctgta gttaaaagtc ccatctcagt
    27421 catcattttt atagcttcta aaacttctac ttgtttttct tgcatacctt ttataactaa
    27481 gcataatggg tcaaatagta tatcagttgg ctccattcct aacattaagc ctttttctaa
    27541 tatttcttgg cagtaagcca tacgctcatc attatctctt gggatacctt cttttgcaca
    27601 taaacctata accattgctt cgtattcagc tgctatatct attaaatcaa atcttgaacc
    27661 agcatcagca gagtttatta ttggttttgc attagttcta tcataaactt ttagtccagc
    27721 ttcgatagct tttttattag ctgtatctaa tgctatagga acattgttga actcagattg
    27781 aagtaatttg attccccatg tcattatttc ttcgccatct ctttcggcag gtcctatatt
    27841 aaaatctata tagtgtgctc ctgcttctaa ttgctctttt gctcttttta agattggagc
    27901 tgggtctctt tctgctaatg cttttcttat tgaaggtgat atacaatgta ttctctcacc
    27961 tataatcata aatttttcca tttataatgt ccccctttaa atttaatatt aatagttagt
    28021 taatatttta ttagtttgtt tggcatgctt tttcttggta tccttttaag aacttaggta
    28081 attccataga ttcctctggt cctataacta cattccatcc tggtaagtta tcttctatat
    28141 cacctttaag tactgcaact tttcctggaa tgataaggtc tttacaatta gttacttctt
    28201 ctaccttact ttctttaatg aaagcagaga ttgagtttcc accaaattta ccagcagccc
    28261 aagatgtaag aactgaatat ccacctgcat ctggtattac taaccatact ggaacttttg
    28321 atctttctat atcaccagca actatgaagt aagttaatgc aaagtcaaca gtaactaata
    28381 ctggagaatt ttcatctggg ttgttgattg gataaatctt tggttcaact ctcattggtc
    28441 tttgtgggtc agtgtatatg ttttgtctta gtgcaaataa tggtaatgcc ttagcataac
    28501 taatatcatc tattactatt atagaaccgt attttatagt gaatattgat gataaagcaa
    28561 cttccatcat agggtttgaa ttagataatc tatttgcaaa tactattgaa ggatatccaa
    28621 atgttctatc ttgctctttt aaagctgttc ttctaacttg tatagcatta gtgtaagtat
    28681 cttttatgtt ttctcctgtt acatctaata ctaattcttt atatccagcg gcttgaatta
    28741 attcaactgt ttcatataat tcttctaagc taccagcttt tacacctaat ggtaaacttg
    28801 ctcctttaac tacttctatc atatctttgt agttctcttt agttgctcca tataccattg
    28861 gtctagcatc ttttaatact tcaacagctt ctttaactac ttgtgcatca tcacaagcta
    28921 aaatataagc tacttctaat ccactgcctt ttattttatc tattaatttt aaatacgcat
    28981 ctttatctcc acaatactct aaaacagcca tttcaacttt catttgttca ccaattctta
    29041 catagtcaac ttttttcatg ttagctattt tagcatctac tgcttcatca ctcatacaag
    29101 tacagaatga aactgcatat ctatttctac ttactaatgt tttttcatgt ctaaataata
    29161 ctgtttctcc acctagttca tactcagatg caccagcacc aacttttaaa gctttcatta
    29221 aaggtgcagt agcctctgat aattttgcta tagcgtcatc agacatatgt gggcatttac
    29281 ctacttctac agctcctgaa gcaaccttca tagagaaagc catacaagtt gggaatccac
    29341 aatccttaca gtttttcttt ggtgttaatt taaatatatc taaagctttt aatgccatta
    29401 ttttttcccc ctaaccttaa caatttaatt aagctaatgc gttaaccaat tcttttatag
    29461 tttctattga ttttggatga cgaagtataa ctgcgtttga accacctact aaaacacttg
    29521 ctgcagttga aacttccatt gctatagttc tttcttctgg acaaccccaa tctggctcat
    29581 cttctattgg agctatagct tctttaacat gacctacttc aaaagctact ggtgttatga
    29641 taggcatttg taatgtctta tcattttgac caaatgctgc aagtctaatt ctatccatag
    29701 tagatgcaac gtactcatat ccataaccaa ctgctgaaca tccaacattc ataactatat
    29761 tttcaccttt aacgcctagt tgagttaaca atacgtttaa ttgtttagct aggtttatat
    29821 caacagaaga ttcagctcct actttatgtg cataagccat accagctgat gctcctactc
    29881 ctttgtagtt atcttctact gctgacataa ataagcagtt atgtccatcc aatgtttgag
    29941 ctaatttttc aaataattta ccatcttttt catgatttcc tgaaccagct acaactaaag
    30001 gtaattttat tgcttcaatt actgccttag ctacatcagc acattcatca actgacttat
    30061 ctaatccatt tgggtcagaa ccatcaaatt ttaaacaaat aaaatctgct tgtgtattag
    30121 cttcaacata tttagcccac tctactggac aattagctac atctttgtat aattccttat
    30181 atgaatcagt ccagctttca ggataaacgt ctgatatttg tatccctatt tttggagaat
    30241 tacctacttc tccatcaaaa ctataaaaag gtaatacatt ttccccacct aatttaattg
    30301 ctttttcccc tattcctact tcaacttctg atatttttcc agaatatttt tgagtagaca
    30361 ttttaaatgc catatttttt tcccccttaa gcctatttta attctaattt agcaaaaatt
    30421 tctttcatag ccactttaga ttttgaatct tcaggtaaat taactaaagg aatacctgta
    30481 gaatcatatt catatatcaa ttcatccata ggtacaacac cgattagctc taggttatgc
    30541 ttttcaattt cttctttaac accatcattc ataactccat ttggaacttt atttacaatc
    30601 aaaagtattc ttccaacact taactttagt tcctcagcta aatctcttat tctagcaaca
    30661 gcttgtatgc tacgtcttga gcaatcacta accaataaaa gtgtatcaac atgtctagtg
    30721 acttttctac ttagatgttc cataccagct tcgttgtcca tgactaagta tttgtaatga
    30781 ccagatattt tgtttacttg ttctctaagt atcccattta caaaacagta acatccttca
    30841 ccttctgacc tacccattac taataagtca tatccttcgc cttcttcgat tatagaattt
    30901 aatctaaatt gaagatattg tgcctttgtc ataccaccag ggaatgcatt gcctaacttt
    30961 tctctttgat ttacttcttc tcttatttct cctattgttg cttcaacttc gatacctaat
    31021 acttcattta tgttagcatt ggcatcagca tctacaacta atactggtcc ctttttgtcc
    31081 ttaaccaaat aatcaatcaa aagccctgtg agacttgttt taccagttcc accttttcct
    31141 gcaacagcta tattgtatcc cattttaaga cttccccctt aaatctagcc acagagcctt
    31201 atgtactctg tctgtctatt ataatttagg tacagaatga acacattctc catgtacatc
    31261 atgaagtgct tccattagcc cttcagataa acttggatgt ggatgtattg catcccctac
    31321 ttgttctact gttaatccta gatgaacagc aagtgacaac tctgtcaata aatctgtagc
    31381 atgaggtcct actactgctg cacctattat cttatcagtt tctttgtcag ctataacttt
    31441 tacaaatcct tggaaatgtc ctatagcttg tgcttttcca agacctctaa aatcaaattg
    31501 acctacattg tattctacac cttcagcttc aagttgcttt tcagtcttac caacaccagc
    31561 aacttcaggt tcagtataaa cacatcttgg aattgctcta taatctacta ccttagtctt
    31621 acctaaagca ttttcaactg ctacgatacc ttctttagaa gcaacatgtg ctaggaaagg
    31681 agtatctatt atatcgccta ttgcgtatat accttctaca ttagtttcaa gatgttcatt
    31741 aactactact tttcctcttt ccatttcaat tcctatatct tctactccag aattgtcaag
    31801 gttaggtctt ctaccaacac atactaatgc atattgtgct tctataacct ttccattaga
    31861 aagagttgca acagccttac catcaactac ttcacaagtt tgtactccaa tacctgtaat
    31921 aaccttaatt ttatctttct taaattgtct aagtagttgt ttagctacat ctttgtcttc
    31981 atttaatagt atttggtcaa ccatctcaac tatagttact tctgttccta aggctctaaa
    32041 gaactgccct atctcacatc ctataactcc accacctact attaacatag actcaggtat
    32101 ctcttcaagt ccaagaactt catcactagt tattacaact tttccatcat atgggaacat
    32161 tcttggtaca actggaacag aaccatttgc tagtattatc ttatctgctt ttatagtttc
    32221 tactgttcca tcatctttag ttacttctat agtgttttta tcaattaatt ttccaaaacc
    32281 attaactaag tttactcctc ttttttcaaa taagaactct ataccactta ttaattggtt
    32341 tacaacttta ttttttcttt ccattatggc agtgaagttt ggcttaactg taccatctat
    32401 ttctatacca aaatcctttg cttcttttac agtattcaat actcctgaag aggcaagaag
    32461 tgctttagtt ggtatacatc ctgcatttaa gcaagttcct ccaactcttc ttttttcaac
    32521 aactgttaca tctgcaccaa gcatagaagc ttttatagct gctacatatc ctcctggtcc
    32581 accacctact actactatct tcatctttaa ttcccccaaa cctaatataa tctacaatct
    32641 tgcaatacta taaacctgcg gcatccagta tagcaggtat attctcttct gccccaattg
    32701 ccattatatg aacaccatca cataaatctt cttcttttaa ttgtttgata agttcccctg
    32761 ccatttttat tccttcactt acccagtttt cttttccagc tgctcttaat ctttcgattt
    32821 gttcatctgg aacaaatatt cctggtacat ttgcagtcat aaatttagcc attcctggtg
    32881 attttaaagg tactattcct gctaatacct tgcaatccat atcccttgtt aattctctaa
    32941 actttctcat tgtatttatg tcataaagtg cttgtgtttg gaaaaattta gcccctgctt
    33001 ttattttctt tttcattttt aataattgaa cttctattgg agaatattct ggagttactg
    33061 atgctcctag ataaaaatct ggagaacctt ttaacttatt accagccatg tccgtacctg
    33121 ccattaaagt ctcagcagtt tgaagtatcc ccacagaatc taaatcaaac actcccttag
    33181 cttgtggatg gtctcctacg ctagtatggt ctcctgttag tgccaacaga ttgtttatcc
    33241 caaatacacc tgctgacaac atttctcctt gtatagctat tctatttcta tctctccctg
    33301 ttatttgaat aactggctct aatccagcat cttttaataa tttacatgta gcaagagaag
    33361 ttgctctcat aactgccgat tgaaagtctg ttacatttgc tgcatgaact cttccaacta
    33421 acggctttgc acattctatt aaatgtgaaa ggtcagtccc ttttggagga gccatttcag
    33481 tagtaactgc aaattttcca ctttctaatg tttctcttaa taagctcatc tctaatcccc
    33541 cttttaacct tctaaaaaaa acttttatga atttgctgct gcttctttct ttttctttgc
    33601 acttaaactt cttggattat tttgcttaga atagtctttt ggtggtctta tttctgctaa
    33661 gttatctaat tgtcctatag cttctagtct ttcatatatc tttatccaag cacaatcatt
    33721 ctctgagttt acttcacact taccattttt agctccacca caagccccat tcataagacc
    33781 tttagcacac atagttactg gacatatccc acctgtccaa ccaagttcac agtctccaca
    33841 agccttacag gcttcttcat attctccaac tctttgtact tcccctataa acaaagtatt
    33901 gttggctgga taaactggct tatcttttaa attcttaact attgtctgtg taccatctcc
    33961 acatgctaaa gataaaactg catcagcttc cttagtctct tcttttagag cctttaaatc
    34021 ctttttagac tttaaaaggt tacaagcagg gtcaagcatt acatatccta agattttttt
    34081 gccttcacct tcaagagttt ccttcatttt taaaacttct tcttccccac cacttttaca
    34141 agttgctgca cattgattac aaccaactaa aaccagcttg tcaaaatctt ttaagtaccc
    34201 tagcacttct tctaatggtt tattttcaga aattatcata tctacttccc ccctaaacct
    34261 tccacatata tatatctatt tattgttttg taatttacaa gccttaacta catgttttgc
    34321 aagtatagat gttgtaactg aaccaacccc cgctggaact ggagttatca ttgatacttt
    34381 gtctaaaact gcatttgtat caacatctcc acataaattt ccttcttcat caacatttat
    34441 accaacgtct ataactaccg ctccatcttt aacaaaactt tcatcaacca tcttagctct
    34501 accaatagct gctattagca cgtctgcctc tgctgctaca cctgatagat ttttagtttt
    34561 tgaatgacat attgtaacag ttgcgtgttc acttaataaa agcattgata ctggctttcc
    34621 aactaccatt gatcttccga gaactgttac cttacttcct tttaaatcaa cattatagtg
    34681 ttttaatatc tcaactactg ctgtaggtgt acatggtgga aatccacttt tatccccttc
    34741 cattacctta gcagagttta ttggactaaa acaatctacg tctttttccg gtgcaataac
    34801 atattttata acttcttcat ttaagtgttt tggaagaggt ctaaaacata atataccatt
    34861 aacatttttg tcatcattaa ctctctttaa tacatcaata tattcctctt gtgttatatc
    34921 ctcagctaac tctaacactt ctgttgttat acctatgttt tgacatctct ttagtgctcc
    34981 tctttcataa gataaatcat cacttcttgc cccaactcta actattgtta gtttaggatt
    35041 tataccttct ttaactagta aatcgacttc ttttattaat tcttcactaa ttttatcagc
    35101 tacaggtttt ccttttataa tttgtccttt agttgacatt ccttccatac attcacaact
    35161 cccctacaag caaacttaaa ttaatttatt ctatatacca catattaata acatagtatc
    35221 tactttcgtc atttaaaaat tattttccaa gagctttttc aactttggca taaacttcat
    35281 cacaaatttt tacaccatct tcaactaact tatctacttc tgtttttacc ttattaacat
    35341 attctttatc ttgtatagaa tttatattta taactacatt taattgtcca ccaagtatag
    35401 cagctcttaa acactgaact cctacaccaa catcacttat agcaagtctt gaacctttat
    35461 caactaaatc ttcatgtaac ttaatagctt catagcaaac tcttactatg tttaatggta
    35521 cttcacaagc aacttttaat gccttttcca ttgtttctgc ttttattctt ttttcttctt
    35581 ctgtttcttt aggaagcccg taagcttttg ataatggtaa gaaacattct gcatcatcat
    35641 ctatcatttt taataaatct ttttctaact ttcctgcctt acttaatatc tcttttacac
    35701 tttcttcata ctcagcatac ttcttcttcc ctattgtaag gttacatacc atactaccta
    35761 aagccatacc tattgcacct actagtgcag cagctccccc accacctggt actgcttctt
    35821 tggaagatag tacttctaca aaatcaacac aagttttatc tgctattttc atgataatcc
    35881 tccttgaaag gatgtaggaa aatatctcaa attgttcgag atattttacg aactatttta
    35941 agatatctcc tactctttat ttataaatta atttccttag aataatccag ctattactcc
    36001 attttcatca acatctattt tttctgctgc tggaactttt ggtaatcctg gcatcttcat
    36061 tatttctcca gtaagtgcaa ctataaatcc agctcctgct gaaattgttg cttgtcttac
    36121 agttattcta aatcctgttg gtctacctaa tttagtttgg tcatcagtta aagaatattg
    36181 agtcttagcc atacatactg gaactttagt aaatccaagt ttttctaagt tagctatttc
    36241 cttatcagct tcaggagtat aatctacacc atctgctcca taaatcttag tagctatagc
    36301 atttatcttg tcttttatag ataaatcatc ttcatagcag aatctaaagt tattttcttc
    36361 ttcatccaat aatcttaaaa cttctttagc tactgcaatt ccaccttctc caccttttgc
    36421 ccatacttca gaaagagcaa cattaacacc taattcctta cacttgtctt caacaagttt
    36481 aagttcagct tcagtatcaa gtgggaatct gttgattgca acaactgctg gtaatccata
    36541 tacttgagtt atgttttcaa catgttttaa taagtttgga agtccttttt caagagcttc
    36601 taggttttcg ttatttaagt ctgctttagc aactccacca ttgtatttta atgctcttac
    36661 agtagcaact attataacag catctggttt taagtttgcc attctacatt ttatatctaa
    36721 gaatttttct gctccaaggt cagcaccgaa accagcctca gttattacat aatctgcaaa
    36781 gtgcatagcc attcttgtag ctataacaga gttacatcca tgagctatat ttgcgaatgg
    36841 tccaccgtgt acaaatgatg gtgttccttc aagagtttga actaggtttg gttttaaagc
    36901 atcttttaat aatgcagcca tagcaccatt agcttttaat tgtctagctg ttacaggttc
    36961 accttcgtag ctgtatccaa ctattatgtt tcctaatctt tcttttaaat caaatatatc
    37021 acttgccaaa cagaacgctg ccattatttc agaagcaaca gttatatcga atccatcttg
    37081 tctaactgct ccatcacctt ttttacccat accatcaacg atatttctaa gttgtctatc
    37141 gttcatgtct acacatcttc tccaagttat tttcttaggg tctattctaa gtgcatttcc
    37201 ttggtgaata tggttatcaa gcatagcagc taaaagattg ttagcagctc ctatagcgtg
    37261 aaaatctcct gtgaagtgta agtttatatc ttccatagga acaacttgtg catatccccc
    37321 accagctgct ccacctttaa caccaaatac tggtcctaga gatggttctc ttaaagcaac
    37381 taatacattt ttgtctagtt tagcaaatgc atcagcaaca cctatagtag tagtagtttt
    37441 tccttcacct gctggagttg ggtttatagc tgtagttagt attaacttag cttttttacc
    37501 agtctctctt tttagtagat tgtagtcaac ttttgctttg tatttaccat aaagttctac
    37561 atcatcttca cctagtccta atttttttgc aacctctcta atatcttgag gcttagcttc
    37621 ttgagctatt tcaatgtcag atttaaatcc cataactgaa tctcctccca caacctaaat
    37681 agatttaaaa tataattttt gtaaaaattg tattaatatg taaaaattat attttataag
    37741 ataattatac atgtaaaatt acttaaaaac tagctactaa gctattaaaa tattcaaact
    37801 taatagctag ttatttatct aattacttac ttattaccta tctattgcct atgtctaacc
    37861 tttaaccaac ttaaaatccc aaatcaaaat ttctatctat tttatttatc cttaagtgac
    37921 attaatttat cttttattgc tttaatctgt tctttcgttg tttcactaga atcatatgga
    37981 gattgattgg ctctatcaga atcaataaca tcttggttat aataaataaa tcctaaagat
    38041 tctccatctt ctaagttttg gattataaat tcttcatctt ccttatttct aattttattt
    38101 cctactacaa atactttatt tacacctata tcatgtccaa gtttcttaac ttttctataa
    38161 gtctgtagac ttctctctcc tggttctaca actactataa aagcatctac accttgggct
    38221 gttcctcttc ctaagtgttc aattcctgct tccatatcca taacgactac atctttattt
    38281 tgtagtatta aatgtgagca gagtctcttc aaaagaacat gctctggaca tacacatcca
    38341 gtaccaccag aatctacggt tcccaatgta agtaatctta ctccattgta ttctttacag
    38401 aaattttcag ggatatcgtc tactttagga ttcatcttaa acatttttcc aaatgaacct
    38461 acagaagcag ctgttctatc tgaaactaat tttttcattt ctgatatagg tactattgat
    38521 tcatatactt cttttggaaa acctagtgct aaagctaaat tagcatcagg gtcggcatca
    38581 acagcaacaa ctctgtatcc gtcttcagca aacatcctag agagcattga agaaaaagtc
    38641 gttttaccta ctccaccttt acctgttatt gctatcttca taatccctac caccttttat
    38701 tactatttgc tttttagtag ttaattttac agttaaatat tttttatact ctatattttt
    38761 atacactaaa aataaactac agctagtcct tacaggaacc aattaaattt accctactag
    38821 cttttcaaag cagcatgtat agttttatta ttctttaata ttgtttatat tttatttatt
    38881 cagtatattc attatatttt gatgttcttt actttaatat ttgactttat tcaactttac
    38941 tcaaccagaa acttcttata aaaacgttta ttctataact tcactatttt ttaagcttca
    39001 gccttttctg ccattttttc ttcaactaat gcttcaaagt gaacacgttt tttctctata
    39061 tcttctatta ttgtagctgc taactctttt ggatttgtat ttacagtaaa tgtagctcca
    39121 actttatctt ttaattcact agttaatata tccactgctc ttgaagaacc agctattgga
    39181 ggcattattc ctaaataagt gtctatacca gaagctacaa catagcatcc tatagcaact
    39241 gctttttctg acatccactc tggagctata cctacaactg gaaggtcaca catatccata
    39301 tctaagtaat tagctgctgc tccaaccaaa tctagtatac gacttatatc aacacaagaa
    39361 cccatatgta gtactggtgg gattcctact aatttacaga cagttgcaag acctttacct
    39421 gcatattttt cagccgcatc agattccatt aaaccaaact tagctgctgc tgaagctcca
    39481 caacctgtag taactactat tatgtcgttt ttgattaatt ctttcattat tgttacgtga
    39541 gcctcatttg aaactccttt agcattgtta catccaacta caccagctgc tcctcttaat
    39601 actccagctt ttagacagtc tgttaatggc ttaactgttc ctgctgggtc tatatgagag
    39661 tttacaactc tgtctaattg atttactact gcttctacac tatacccaac tgttgcgcct
    39721 gattttaatt caggtataag tacttttgat ttatctctat ttttaaagtt taaaatagct
    39781 tctttaacta ttgctttagc atcatcaagt gcttgttctt ctctaaattc aatataagtt
    39841 gaacctgtta tcttagcttt tggagaagtt gttacaaatt tagtatggta acaatctgca
    39901 actctagcta atgcagggaa tatacattga acgtcaacta ttacagcttc aactgctcca
    39961 gttattattg caagttcttg ttgatggaag tctcctgcta tttttacacc atgtctcatt
    40021 gtaatctcat tacctgtaca acacatacct gctaagttta ttccatcagc accaacttct
    40081 ttagctaaag ctactaagtc tggctcttct gatgctaaaa ctatcatttc tgataatgct
    40141 ggctcatgtc catgtaatat tatgttaact ttattttctt ctaatactgc taaattcgcc
    40201 tctgttcttc ttggcactgg agttccaaat aagatatcgc ttagacgagt acctatcata
    40261 gaaccacccc aaccatcagc catagaagtt ctaagactca tgtgtattaa actatctata
    40321 tcaccagtac atcctatatg agtagagtgc attattgttg caatttctct atctatagct
    40381 cttggctcta tagcgtattc tttccatatt ttttgtcttg gttctggtgc attttttata
    40441 aatcttaatg tcccaaaagg ttttccaaat tccattaatg caatttctgc cacttcatgt
    40501 gctacatcat agatatctct accttctgtc tctacatccc attctttagc taaagtaatt
    40561 aacttagctt cgtctcttat tttatagttt ccatttggat cagctaatgc taatgtatgt
    40621 gctatatctc tagcatggtc tgaatgggca gctgtcccac cagcaaccat tcttgcaaag
    40681 tttctaccta ctatagtatg ctcatctgct ccacataacc cccttggagt cttagggctt
    40741 attctacatg gtcccattcc acatattcta cagcaaacac cttgaagacc aaaaccacat
    40801 tgtgccttaa agccttcttt tctatcatac attgtttcta cgccttcttc tctagcttta
    40861 gctatcatct tctggctgtt taaatcaatt gttaacattt tttcatccat attcctatcc
    40921 cctttttttg atttttaaaa tcccccaggt gactgacttc atgtcataaa ataataaaat
    40981 aaatgctttc taagttgatt atatactaat tttcagtgct ttgctttaga tttaaatatt
    41041 tttttaacaa aattagataa tacataaaaa ttttattttc tacacttaat ttaagttgat
    41101 tttatcgctt gtttttggct atttgttaat atctaaacct acttttttga tacttcagtt
    41161 taattaaatt atgttttgat ttttttaaat caaatttgaa atttttctag tcatttttat
    41221 attattgcag attgtttgtg tatttattat ctttcactac tgtttggact tattacagat
    41281 ttttactccc taaacttaat tagtaaaaat ttcttagaat agcatttttt ggactaattt
    41341 ttttgaattt ttttattttt cctactttaa tattaattat agcttcaaat tgaacatttg
    41401 tatatattca tttgaagcta taattatttt ttatataaaa cttttttaat acagcattaa
    41461 tttagacatt ttgactattt tttctacttt aattcattta tttcaagcga tttatcctat
    41521 ttcatatttt tagctacaat agccccatat gcaatagaat ataatttaga ttcatgtgaa
    41581 tcagctcttg acactaaaac tataggagct tttgcaccca taattatacc tgctgattta
    41641 gagtctgcca tatatgtaaa ggctttccct attccattac caacctctat tgttggaacc
    41701 aatatgatat ctacatctcc agatacttca cttttaaacc cttttatctc actagcttct
    41761 tttgaaacag ctaaatcaaa agccagagga ccttctacaa ttacattttc accaaattct
    41821 ccttctttac aagcttttgc taacatatct gcatctacag ttgcttgcat cttaggattt
    41881 actttctctt tagctgccaa acatgcaact tttacagttt ccattcctaa agcttttgca
    41941 gcttttatag aattttttaa tatctttgcc ttttgttcat aatcaggagc tatattcata
    42001 cctccatctg tagttataag taatttatgg tatttttcta actcatatac cataacatga
    42061 cttaataaac tatcagttct aagaccatag tctttattta acacttcttt taatatgaca
    42121 gatgtatcta ataatccttt cattacaaag tcggcttctt tacttgatac taatttaact
    42181 gctatttctg cacactcttt gatatcatcc ttatccacta ttttgatacc acttaaatca
    42241 aatcctattt cttttgatat ttctcttatt ttgccttcat ttccaaccaa tataggagta
    42301 attatatttc tttctacagc atcctttatt gccattaaaa cttctttatc atgagctgct
    42361 gctacagaga gtacaatttt ctctcctccc ttaagttgtt ctaaaatatc atctaatttt
    42421 tttatcaaaa gtaacccctc ccttatatat atttaactaa ataataccat atttgctata
    42481 ccttttctca atttattttt tatgttataa agaatttata taactaaaag acagtttaat
    42541 attttattct gggatttcat caataactct taaaagcttt gcaggaacac cacctacaat
    42601 agtattttct gcaacatttt ttgttacaac tgctccagca gcgataattg cattatctcc
    42661 aatagtaact cctggaacta cagttacatt agcaccaatc catacatttt ttcctattgt
    42721 gattggagaa ggataagttg taccacgttt ctctggtgca ataccatggt taagtgtagc
    42781 caataccaca ttatgcccaa tttgcgtatt atctccaatc tcaatccccc cctggtcttg
    42841 aaaatggcaa ctgctattta taaaaacatt ttttcctatt gtgatgtttt ttccacaatc
    42901 tgtataaaat ggtggaaaca taaaaaatga ttcatctacc ttttttccaa ttaactcaga
    42961 aaataaacta cggacttctt ctggttcatg ataagaagaa tttaacttac ttgtgatttt
    43021 cattgcctca ttactcaact taaccataaa ttcatgtatt tctgatttac tagaaacagg
    43081 ttttctttga ttgatatgtg ttaaaaattc attgatattc attttataca tcctttcaat
    43141 atgtgcttta atttatttgt acattctaaa taatatttta ataaaaatta atttaattta
    43201 tttttattaa aatatcattg cttcttttag tgtaatatac aattatatct atatcaaata
    43261 tctatattaa atacttaact atacttaaaa agcatttaaa ttgatataat tatataataa
    43321 ataagttaat attgaggagg gataattatg gaaataagag tattaagata ttttttagca
    43381 attgcaagag aagaaagtat atctggtgcg gcaacctttt tacatataac acaaccaaca
    43441 ctttctagac aaattatgga acttgaagaa gagttgggag ttaaattatt ggttcgagga
    43501 aatagaaaaa tcaccttaac aaatgaaggt aggcttttac ataaaagagc agaagaaatt
    43561 atatcattag ttgataaggt tgaattagaa tttgacaatc ccaatgagat tattagtggt
    43621 gatatatata taggaagtgg tgaaactgaa gctatgcgac ttatagcgag aactgcccat
    43681 cattttcatc aaaaataccc caatgtaaga tatcaccttt ttagtgggaa tgctgatgat
    43741 gtgactgaaa aattagacaa gggactgctt gattttggta ttttaattga acccgcaaat
    43801 atacagaagt atgattatgc acaaattcct gctactgatg tatggggttt actaatgcgt
    43861 aaagatagtg cccttgcatc acaaagcaca ataaatccaa aagatttatg caatatacct
    43921 cttattattt caagacagac acttgttgat aaagcaattt ctaaatggct tcaaggcagt
    43981 aatgaaaaac taaacattat tgcaacatac aatttagttt ataatgcttc tcttttggta
    44041 gaagaaggtc tgggctacgc tctgtgttta gatagactcg tcaatactac agaaaacagt
    44101 aatctttgtt ttagacctct atctcctcct ttagaagcta aattaaatat tatatggaaa
    44161 aaacatcaaa tattttcaaa ggcagcagaa aaatttttac ttcaaataca aaaagatttt
    44221 tcttctatca tgttggatac atctaagttg gatacaccca aagaaaaata aatcataata
    44281 aaaagtgaga gtgtctcaaa atgaactttg cactgtataa ggcactcttt tttatacaaa
    44341 gtaaaatata tgttcatcat ttcagcacta aaaaagaggc ttatctctat tttgagatag
    44401 ccccacttaa ccaataaaat taaatacaac ttaacaatat ccttacttat ttattccaag
    44461 ttctcttaga caagcaatag taatttcatc ttgtggattt aattcataag ctctttctat
    44521 atagtaagtt gcatcatcaa tatcaccatt atttaaataa gccattccta aattgcaaag
    44581 tatttctggg tcttccttta tcttagccgc ttgttcaaaa tactctattg ctttatctaa
    44641 attattctta taagcctcac aaagccccaa ttccactata gtatccactt gattaggctt
    44701 tattatcaat atcttctcaa gatacatctt agcctgctct atttcgtcca tacccttata
    44761 tccaagtgct atcataaaca gcaagttcca ccaatccata tgttcttctt ctaatggaag
    44821 taacttctct aatccttcct taaacttacc ttggaataca agtgtatatc cttcttcata
    44881 ttgaacttta aagtccatct tgcccagatt ctcttgtacc tcagctacta aatcagcatc
    44941 taaccctagc cttaacgctt cttcccaagt cagcttagtc tttaaatatt gaccttgatt
    45001 ataataatgg tagcctaagt gataataacc taatgcaaaa ttctcatcaa catcaattac
    45061 cttttcaagc ttagccatgg cttcaagtaa gaatgcattc attgccttat catccatatc
    45121 tttttgatac tgtttagcta tctcttggca aacaatcgca tagttataca ttgcatctaa
    45181 atcatctgga tacattgtta taaatgcttt tatatatatt aaagaatctg tatgttcacc
    45241 aatctcaaag tattttctcg acatatatcc catatacgat ttcaaatcca aatcaatatc
    45301 ttttgataaa gcatttaaaa acttctcata ctctgcattg tttttaaact tactatctat
    45361 tccaattata tatatcattg catctgctat agacattgaa tttaacttat cttgagcagt
    45421 cttttctttt atccctttta ctaatacctc attttttata ggtacatcta atccaccttt
    45481 tggcacttca tagcttttta atttaaactc tccaccatgt tttatagtta taaaagctaa
    45541 ttcttctgct ttttttagca aatatttttc tatcttaaaa tccattttat cctcctaaat
    45601 acttaaatct ctactttatg agttttagat ttctcaaata tttttttatc atcatactca
    45661 aataagacta cattttctac ttctcttatt tctgtattta tatcattttt taaatccaca
    45721 acattaaatc taaatggctc aaaatgaaca tattttatta tatgtttagc tggtttatca
    45781 acaaaactag gtaaatatgt tgataagtta tcattatctt gtaaaaatac atggcatcta
    45841 ttcttaaaat catccacatc tagcttatta aaaaactgtg gtatatttga tgttttaccc
    45901 agtgatatat agtcatattt taatatatcg ttgaatacat cattatctat gttgattttt
    45961 tcattataga aatccatcaa tattttatat aactgattct ttccttgtgc caaatcaaag
    46021 tagccatttt catcaaaata cttggcaaaa acctcaaaga atttaaaagg gctttgttta
    46081 taaaatctgc ctataatata tctcatagat aacacaaagt ttttagaatt gtagtatctc
    46141 tccagtatat cttctatatc ctttagcttc aaagtttctt catagcttat ataattattg
    46201 tataaaaact catatggagc ataatcctta tatttaaaac catgctcatc tgcaactttt
    46261 ctcatacctg tccctttaat catctttaaa aagcctaatt gcagatgctc tatacctaga
    46321 ttaaatacat cattaaaaga attttcaaag ctcttataat cttcataagg cagtcctgca
    46381 attaagtcta agtgctgatg tatatttcta taagatgcta cagtttgtac aacatgagac
    46441 aactttttaa aatcatccct tctgcctact gcatcaagtg ttttttcatt cgttgactga
    46501 acccctattt caaattgaaa aagaccttct ttacaatcag ccaaaaactc aagcatctta
    46561 tcatctatca aatacgctgt aacttcaaaa tgataagttg tatatccatt atcatttttc
    46621 ataaggaaat ccatgatttc catagccact tttctatttg cattgaatgt cctatctata
    46681 aactttatct gtgatactct tgcatctatt aaagctttta aatcactctt tatcctgtct
    46741 aaactaaaat accttagacc tggaatagta gatgataaac agtactggca gttaaaaggg
    46801 caacctcttg atgattcata atacactatt ctattttcat attcttttgg attcaagttt
    46861 tcataaggac taggaatctc atccaaatct tgaagtaatt ccataggctt attcactatt
    46921 atctcattgc cttgtctata tacaagtcca tttacatcat ttatttccat ctttccttgc
    46981 aagtgaagta ctaagtctct aaaaattaat tctccctctc cataaagtat ataatccaca
    47041 aaatcatact ttttcattgc ctcataacta tcataactca cttccggtcc gcccaaagct
    47101 attttagtat taggtctaat tttttttaga ttatcacaaa gtttaactat atctcccaca
    47161 ttccagatat atgttgagaa taatattata tcatattcat ttttatatat atcctttaaa
    47221 atatagtcca aatcgttgtt tatcgtgtat tctttcatat taacttctat caaatctctt
    47281 acaaattctt ttagatatct tatagccaaa tttgtatgaa taaacttgga atttaatgtt
    47341 gttaataaaa ttttcaaata tttctcacct ctttttaaga ttatactgtc tttttgtacc
    47401 ccttataggt taaaatagta tatgtaaagt ctaaataata tctatataat taatttatta
    47461 atttcaagta atgtgaggaa aggaagtttt ttatggaatt aattgaaaaa gtatctatca
    47521 actctttatc aaaaagagat ttactactta ttataaagtc tcttgaatac actaatgaaa
    47581 atactcaaat tagtgatttc attgacctta ggaataatat agtaaaggaa ttatgttttt
    47641 taactgacac taaggaaaaa gattttttag attatctaga aaaaaatagc acactctaat
    47701 aacctaaacc tttttaaact ttaatatata aatacctgat ttagaattta tttttatgct
    47761 taatatatta tcattaaagg ttttcgacat aactgttatt tgattaaata tagggtctac
    47821 tatagtatta ccttcttcaa gtcttataag acttcttggg tctatgcttt tattatatgc
    47881 cttaactcca attttatatt ttctcaattt aacaacatat tcggacatag aaaaaataaa
    47941 atctatatta tcttcattta atttactatc ttcacccaaa atggataata tagatttttt
    48001 tatgctatta attccttcat tttctacagt tctatcttcc atatatacat catatagaac
    48061 atttaaaaca tttaataaaa tactttcttc atcataatct tcagatttca aatcaatgta
    48121 cttatcaatt aattgtattg ctttaatcct tgccatctga aattttacaa catctgattt
    48181 gtctaattct atagaaaatc ctattatctt gtctttaata ctctgtagta attcgacata
    48241 ctctatttta ctattattta taagattgtg cataatggaa ttatacacta cagaacctaa
    48301 taaataatca aataaaagtt cttctttttt aaaataaata acatactttt ttattagttt
    48361 aataacctca tcttcacttt tttgaatatt agtatttgat aaaattagtg gtaataaaat
    48421 atttaaaaaa ttgatatttc ctattttttc tccataaaat ctatagtaaa taagttcatc
    48481 tctcagtcta ttttcgtcta tatacacaca tctttgcgtt ttatcatcta ttaaggattt
    48541 tgtatttatt atagttaaat ctttaagagc cctacttgca ctactaaaag aagatgtgta
    48601 gacttttttt agtaattcgt cattcataaa tatatccttt ctgaaaaatt gaatgtaaat
    48661 aaattgttat taaaaaaaaa gagacagccc gtttaagaag ctgtcatata tagggggata
    48721 gtagtgtaca aaaatcattg taatctacta tctatatatt attatacgat actacttttt
    48781 tatatgtaaa gcatcagttt tcgacatccc tatattttac agttatacaa attgcgtact
    48841 aagttcaatt ttattctttt tatttcaaca ttttatcatg ctacattctt tcaggagcgt
    48901 gcattcccaa caatttcaga gaattttcta aagtttgttt tgttgctaaa actaatgcaa
    48961 gtcttgcttt tcttatttcc aaattttcta ctaaaattgg attgtcatga taaaatttat
    49021 taaacgcttg agctaaatct agcataaatc tagtcactat atgtggctca tttttcttta
    49081 gagcaagtaa aatattctta ttgaatgatt ctataactct aagaacttct gcactatctt
    49141 catcagctaa tagactataa tctatatcag aagtcacttc tacttctgct tttcttaaaa
    49201 ctgcacagca tctagcatgt gtatactgaa catatggtcc agtctcacca tcaaaactaa
    49261 gcgttctttc ccaagagaag gtatagtctt taattctact attagataac tcttggaata
    49321 ctactgcccc aacacctact tgcttagata tttcatccac atttttagca tttgggtttt
    49381 tagctagtat aatttcctta gttttatcta tagcttgatt taaagcatct tctaaaaata
    49441 ctactcttcc ttttctagtc gacatagtac cttcttcaag tgcaaccata ccaaatccaa
    49501 catgaattaa atcttttgcc caatcatatc ccataagctc aattacctta aaccattgtt
    49561 ggaaatgtaa gttttgttga gaaccaacta catatataca cttgtcaaaa tcataagttt
    49621 cttttctata tattgcagca gctaaatctc ttgtcatata taacgttgaa ccatcattct
    49681 tagtaataag tgctggtggc attttatact cttctaaatc aacaattctt gctcctttag
    49741 actcttttaa aagatttttc tcttctaata gttcgataac tctgttcatt ttatcagaat
    49801 aaaaactctc tcctgctaaa gaatcaaatt ctatatctaa aagtttataa actctgttaa
    49861 actcttttaa actctcattt ctaaaccatt gccataattc ttgtgcttct tcgtcaccat
    49921 tttctaattt attaaaccat gctcttgctt catcttccat ttctgggtgt tgttcagctt
    49981 catcatggaa tcttacgtat aattttagca attctggaat tggattagac tctactactt
    50041 ctttttctcc ccacttttta aaggccacta ttaacttacc aaactgagtt ccatagtcac
    50101 ctaaatgatt tattcttata gtcttgtatc cttgcgaatc atatatctta tataaagcat
    50161 tacctataac tgtagttctt atgtgcccta tatggaaagg ttttgctata tttggagaag
    50221 aatattcaac tattacagtt ttatcttttc caaaatcact atgtccgtaa ttttcttttt
    50281 cgtctaaaac ttttttaatt acagtttctg ctaattgaga tttatttaca aagaaattta
    50341 catatccgcc taattgtatt acttttgtta tctctccact tggttctata ctttccgcca
    50401 attcacttgc tatcatgttt ggagcttttc taaacacttt ggctaattta aagcatggaa
    50461 aagcatagtc ccccatatcc ttatttggtg gaacttctat aagtgcctct atctcttctt
    50521 tcgataaatc ttctattttt tctttaaggc aatttgatat tgccacctta aaatcttgca
    50581 ttctgttgct cctctctaca ttgttcctgg tttatattgt gcttatctct ttatgttaaa
    50641 tcaaatttcg tgttttttca agtatttcat tacagtgtaa aacactttag taattatgtt
    50701 ttctgtatat attaaaaata ctataattgc agtttttaat cacactatta ttgatttttt
    50761 ataagacttt tttctatttt attgtttctt gtactatcca gttgacttta tatacttttt
    50821 ttaatctttt atctattttc atacgtcaat gctaatttta tatacttttc ttttaatctt
    50881 ttattaattt tcatacatca atgttaactt tatatacgtt tttttaatct tttatctatt
    50941 ttcatacatc aatgtcagct ttatatactt tttttactct tttatctatt ttctataaat
    51001 ctttttcact cttaactttt attccttctt cttctaatag ccttgctgtt atacctttcc
    51061 ctggtatctt tttacctgaa aaactaccat catatattag attacatcca caagaggggc
    51121 taccctcttt tagtattgct tcgctacata catataaatt tgctattttt agagtttcat
    51181 tagcccctct tacaaagttt tctgttacat ccttattatc tttagtaatt acttttccat
    51241 caactatctc agcaggtggt cttggtgtag atagaccacc taactgctct gggcatacaa
    51301 gtgtaaattc tttgcctttt aaatattcct tgactttttc attatcatta ttatcgccac
    51361 tatatttaca atttattcct attaaacatg cagataccaa tatcataaaa atgtgctcct
    51421 atacaaatta tatatattct catacaaatt actaatgtat tcttatacaa attattttaa
    51481 tttaacaatt gttactcctg ctccaccttc tccatattga ccacctcttt gtgattttac
    51541 atgtctattt cttcttaaaa tgtcttgtag ccctgctttt aatactcctg tacctattcc
    51601 atgtattact gttacagact ctaaacctgc cacatatgca tcatcaagat acttttctac
    51661 ttccattata gcttcttcta ggtttaaccc tctaaggtca acttcatttt ttacactacc
    51721 agattttgac cttataatgt ttctcgttga tttagttaca tttgttgaaa catctttgcg
    51781 tgttttttgt aaagacttaa aaggtagagt catcttcatt ataccaattt gcactactgc
    51841 ttcttttctc tttttatcta ctgaaactac actaccattt tgatttaaag ttattacctt
    51901 aacttcttca cctggcttta agtcttttat ctccttattt gatacctttg gaactatcat
    51961 actttttact gttggctgaa gacttcccat agagctggtc aattcttttc taagttcttc
    52021 tatcttacga ttcttttctt ttgaagctct ttcttgttca agacttctaa gttcttttat
    52081 tataatatct acttcttctt tagcttgtct tataattgaa aatgcttctg atttagcttt
    52141 ttcaatcatc ttatctcttt gactgaccaa tttttcaagc ttttcgtcat actctacttt
    52201 cagtttttca atctcttctt ttaatctttc agcttcttct ctatcttcta ctgctttgat
    52261 tctattcttt tcaacattct gtaacacatc ttctaaagct atgttttctg tatttatata
    52321 ttctttagca cgtgaaatca cataatcact tagcccaagt tttctagaaa tctcaaacgc
    52381 atttgatttt ccgggaacac ctataagtaa cttatatgtt gggcttaaag tttctatatc
    52441 aaattcaact gctgcatttt ctacacctgg tttagtaaga gcataatttt taagctcact
    52501 atagtgagtg gtagctatac atttagctcc tacagaattt atatcttcta atactgctat
    52561 tgcaagggca gctccttcaa cagggtctgt tcccgctcct aactcgtcaa atataactaa
    52621 agagtctgct gttacgtttt gtaatataga tactatattc gtcatatgag atgaaaatgt
    52681 tgatagactc tgttcaatac tctgctcatc tcctatatct gcaaatacat tatcatatac
    52741 acacatacta cttccataat cagcaggtat atgaagtcca ctttgtgtca taagtgcaaa
    52801 tagacctaca gtttttattg taacagtctt accacctgtg tttggacctg ttatcaccaa
    52861 tgtatggaag tctcttccta aataaattgt atttgctact accttctttt tatccagcag
    52921 tggatgtctt ccatttttta tgtttaaata tttatcctca tttaaagttg gttctattcc
    52981 tctcatctgt atagaaagtt ttccctttga aaatgcaaag tccaatcttc caagtatctc
    53041 ttgattagaa attaagtctt cactaacttc tccaaccata gcagaaagct ctgataaaat
    53101 tctttctatt tcttcttgct cacctagttt taactgtctc aattcattgt tcatctctac
    53161 tatagtcatt ggctctataa aaagtgtcgc accagaagat gactggtcat gcactatacc
    53221 agctacttgc gacctatatt cagattttac aggtactaca aatctatcac ctcttagaga
    53281 aattattgca tcttgtaagt acttttgata agtagttgaa cttattattg aatttaattt
    53341 tgacctaatg gattgatttt tttgggctat tcttcttctg atatctctaa gtgtacttga
    53401 agcattgtca gatatttcta cttcactaat tatagcattg tatatctggt cttctatgtc
    53461 cttgtataca tatagagaat ttgatagaga ttgtattatt ggataattaa aatcttcttc
    53521 atcactacta gataagctat ttcttaatct tctagccact cttaaggtat ctgctatcat
    53581 aatcagactt cctgggtcaa gagaagcacc tatataagct ctctttacct tatcttctat
    53641 atcatgaata ccttccaacc ctactgaacc tctctttata ataattgatt gagcttcact
    53701 agtttcttct agcatagatt tcacttctac aaaatcagta tttggtacta aattttctat
    53761 atattttaga cctaaagaag atgaggcttt cttctttaac aaatctatta ttttattgta
    53821 ttctagaact cttaacgatt tctcattcat tttctatatt caccatcctc gatattttaa
    53881 cttttttatt taattttaaa aatgtatgtt ttcaagcatt tccaataaaa actgcaagtt
    53941 ttatttcttt ttcatttcta aaattattat attttttctc ttttagatat attttacaca
    54001 tattttgtat tatactatgt atgtagctat ctatatatta tacataaaca tgctttattt
    54061 gtaatataat ctgttaaaaa atcttcttta atatttttaa aggagtttat tttagcttat
    54121 aaaaaaaaat agctacttat atagataaat taaggaggaa catgagtcat gggaaacgta
    54181 ttagaaggat taaaaccaga aagtgttttt aaaaactttg agaaaatatc tcaaattcct
    54241 agaggttctg gaaatgaaaa aggcataagt gattttttac ttagctttgg taaaaattta
    54301 ggtcttgaaa ctattcagga tgaaagctta aacataataa taagaaagcc tgctacaaaa
    54361 ggatatgaaa attgtccagg tgtagttctt caaggacata tggatatggt ttgcgaaaaa
    54421 gaaaagaatg ttgaacatga ttttcttaaa gaccctataa aattaagaat tgatggtgac
    54481 atgatatatg ctactggaac tactctagga gctgacaatg gtatagctgt tgctatgggt
    54541 atggctatat tagaagataa tactttagag catcctgcac tagaagtttt agttactgtt
    54601 aatgaagaag atggtatgaa tggtgcagat gcactagacc ctagcttaat taaaggtcaa
    54661 tatatcctaa atatggactc cgaggaggaa ggatacttat tagttagctg tgctggtggt
    54721 aaaacttgtg ttgttagttt acctgttgaa tacaaaaaag ttgaaggtga caaacaagga
    54781 ttattagttg aggttactgg actattaggt ggacactctg gtatggaaat tgttttacaa
    54841 agagcaaatg ctaacaaagc aatagctaga gtattaagtg tattgaatgt tgattatgaa
    54901 ttagcttctg ttgatggtgg tactaagcac aatgctatac ctcgtgaagc taaatgtgta
    54961 atagctgtta ataaagctga tgttgaatct gctaaaaaac aaataaatga cattttaact
    55021 gcatttaaac atgagtttac tacttctgac cctggcatga cttactcagt agctgaaact
    55081 tctgtagaca aagttcttac taaagattgc aaagaaaaag ttgttcaaat gtcttgctta
    55141 actcctcatg gagttcaatc agtaagtctt gatatagaag gattagttga aagttctact
    55201 aacttcgcta taattgaaac taaagaatct actattgaat ttttaacttc tgttagaagt
    55261 tctgttatgt ctattagaga tgaaatcgct gatagaataa gacttttggc tcaagcttta
    55321 ggtgctaatt acgaccttat agctcaatac ccagcttggg aatttaaaaa aggctcaaaa
    55381 ttagaaaaaa tatgcagtga aacttatgag aaattaactg gtaaagttcc tactgttatg
    55441 gctctacatg ctggtcttga gtgtggatta ttacttgata aacttcctca tgctgaagct
    55501 atctctatag gaccagatat gtttgatgtt catactccaa atgaacatgt tagtatccct
    55561 tctgtagcta atgtttggga ttatgttatt gaaatattaa aatctatgaa ccaatattaa
    55621 atttaagttt tgattctaag tagattttaa ttttaagtaa tctatataaa attaactctg
    55681 taaattaata ctactgttag tataatattt actttcaata ttaacttata gaaataaagc
    55741 ctaatgatag ataataacat gtatcattag gctttattgt aatttaaata ttattaagct
    55801 ttattctgtt ttaaaattaa ttcttatact attttaaatc tatttcttcc ttgtctctta
    55861 gaatcataaa gagcattatc tgctatttga tacagttcat catactcttg accaggcttt
    55921 gctataacta cccccatact aatagataaa ctttctccat ttggcaactt aatgagatga
    55981 atatgattgt atacatcatt acatttatta ataagttctt ccttatttat tatatcattg
    56041 atataaacac taaattcatc tccacccaaa cgacctacta tatcattttt tgcaaaactt
    56101 tcatcaagta cctttgcaac atcaataagt actttatctc ctaacaagtg accataagta
    56161 tcattaatcg acttaaaata atcaatatct ataatcaata aagcaccatt tttttcagtt
    56221 gataaattca acaaattttg tgcagttaat tttctaatat atgctgcatt ataaatattg
    56281 gttaaactat ccctttgagc cttatctaac aatctattct tttcctcttt ttcagaatca
    56341 atattacata ttttcccgat tacatacatt ggtatttttc tatcattata aattattttt
    56401 gaagtaactc taatccaatg aaaagtatca tcaagcataa tagaatacat ttcagttgtt
    56461 ccattttctt gtgattttat tacatcaaaa aactttttat tatattcttt ccttatttta
    56521 tctctttcat tttctatatt gccattttca ctttccttat catttttatc caaaccataa
    56581 taagtctcaa tacccttttc tggcatagaa attatcaatt tatcttgctt ataatcatac
    56641 tcaaaaaagt attcacttgt catttcatac aactgagtat gttttttgag ttctaaagat
    56701 atcttctcgt tcatacttgt acgagtatgc aaaccaaatg ccataaatcc aataatacaa
    56761 attaataaac tggctacaat aaaaatagtc tcaattggat tctgttgcat aatataaagc
    56821 aacgagaagt cttgtctata tgtagtattt tggtagacaa ttgattgcat tttttctgat
    56881 gaaatactta taatagcttt attaaatata ttcaataatt cttgtttatc ttttttaatt
    56941 ataccaaaac aaatactctt tgctttataa gtttgaggaa ctagacgcac atttttatac
    57001 ttaggttgat tcatatagta ctgcatagaa taaccttcgc cataagtgta atctgcatag
    57061 cctttattaa cagcttctat acactcttct aaattatcat accaaactat attctcttca
    57121 aactgaccat aataattaat acctttggct agagctacac gttttccatc taaactattc
    57181 tcatttaatt tattattaat catcatagta tattgagtag aaagaaaagg ccgagacata
    57241 actatattat gttcacgaga gtattcataa tcataaatca ttcctgctac aatatcaatt
    57301 tcacgctttt tagtcatgtt ttctaaatct tcttgagatt ttacaggcac aagttcaaat
    57361 tctaagcctg ttttatcaca tatatgattt aacaaatcaa ttgatatacc tcttaattca
    57421 cctgtatctt tatctatatt ttgaaatggt ggtttgttta aaagtactcc tacttttaat
    57481 ttttctgtct ctttaatgta atcagtttct tcatcagata aaaacatttg attgttctct
    57541 ggtgtaaagt atttttcata taaagtagtt gaaaaatatg ggtctttctg ttcaatatta
    57601 ttaattgctg agttaagctt ttgagttaaa tttgtactac ctttaggagt aatgaaataa
    57661 aatggttttg atgaaaattt tgctacagta cgtacacctt ctagtgggtt catactcaca
    57721 ttaagaagta catctgcttt accactcttt agtgcttcta gttgctcatc atttgtttta
    57781 caatatacca attcaggtga cataagattc atcttacaat actcatctaa ctctttaagc
    57841 ctagtatcag aattttctat tacagcaata cggatttttt tcacatcttg tgaactcatt
    57901 atgttatcaa tatctgtatt ttcatataat gcttgtagta ctgtatttgc tactccataa
    57961 ttattccctg cataattata tattttttgc attggctcat tgtataacat acctcccatt
    58021 atatcaattt caccattttc taacatcttc attaattttg taatagattc atttatatct
    58081 ccaggaactt gaacaaattc ataattccat ccagtatact gtgcaatttc tagtaaatat
    58141 tcatatgtat acccaaaata gttaccttgc tcatctattt cagttaaacc tttttgaata
    58201 ggataagcaa cacgaatggt acgattatca ttacctatag cgttgatacc aaaagaaaat
    58261 atgcaagaag tgaatataga tgtaattaca ataattaata aaagtaattt ttttaaattc
    58321 actacacaat cctccatata gttaagattt acagtataaa ttactgcata aacttgtaga
    58381 catgtaaaaa tagattagta tataaaagga tatataaatc tacttagaaa tttaaatatc
    58441 cttaatatat acaatatatt taaattataa ctcatttata tgtctattac aacaaattta
    58501 ccctgctaaa tacctactct atatcccata attttacaag ttgtagtgaa gtttaagttc
    58561 tatctcacct caaatttagc agtgtttcta tttacagttc ttttatattt cacatctgga
    58621 taacccaata taaatgaagt gataacagct tcgttctcgc tcattcctaa cagttctttt
    58681 atctttggct ctacatttga tgccataaca aaaaatccat tataacaaat tcctaatcct
    58741 tgagaatagg ccataagttc catattagat gcagccagtc ctccatctac ataagcactt
    58801 ccacctagag acatatctcc tacaactacc ataagtagtg gggcatcata aaataaagaa
    58861 tcatttccgt tctctttata tcttttgtac attttcttaa atgtattttt atagatagaa
    58921 cgtacagggt cattgtcatc agtatctaat gccaaattat aaagaccttg tattgccagt
    58981 tctttcactt cttttagctt ttcttttact aatatgtatc ttataggctg tctattacca
    59041 cctgttggtg tgtatctacc agcttccact atgtttttta ttttttcttc ttctacctct
    59101 atatctttat attgtcttat acttcttcta aatttaataa aattcaataa cttttcgggt
    59161 tctaattcaa aactttcacg attgtattct attacttcat ttttatcaaa tccttgcatt
    59221 tctattgcat ctgttgggca aattgccata cagtgaccac agttaataca gagaatattt
    59281 ttaacctttg ctttatcatc cttaactttt attgcattta caagacagtc tgattcacac
    59341 ataccacagc ctacacattt ttctaaattt atgtctatca ttccaaatta ttcctccttt
    59401 taaaaaattt actttagtat aaaacaaata gttgtctaag tcaattttta tttttttaac
    59461 aatatactag ccttaactac actttaatgg agaaaaatat acataaattg gaacttaaat
    59521 tagaacttat tcaaagtaaa agataaaaga agtagatgtc taaaagtgaa ctttttagtt
    59581 aattagatat tctttattgt atgaaatcaa gtggatattt atcatttcaa caataaaaaa
    59641 ggcttacctc tattttggat atgtccctgt caagtagaca gactaaaaaa aggctatctt
    59701 tatgcactga gatagcccct tttcccaact actattgttt atcttaatat tagtcaataa
    59761 atttaatttg ctataacctg taagttacat tttaatttga tgtaacttta aattacattt
    59821 ttaagtaata tctttaaatt atattcttaa aatatatcat taaactacat tttaattaat
    59881 ctaaaaacta tatcttaatt aatctaagaa acttacatta taatctttta gtttcccatt
    59941 atcatataca atctttgcac taaagatatt tccagtctta gcccaattta agaacagcct
    60001 gtcaccttcc cacatattta aatcgaatat tttatcatta tcaacccaaa ttaaatttcc
    60061 ttctttacaa tcatttaaat ctttttcaga taattcacct tcaaactcat ctgagaaata
    60121 caaaaacata taccaatctt cttcatcttt aaattttgga aaagttatca accctttata
    60181 tgacaatgat ttaagagtaa gaccagtctc ttcttttact tctctaatta cacattcttc
    60241 tggggtctct ccttgttcaa actttcctcc aactcctaca tacttacctt catgtatatc
    60301 atctttcttt ttatttctgt acaacatcaa agtcttgtca tctttttcta tataacaaat
    60361 agttgtaagt ttcatgtatc tcgccttgct ttctctttat attctcaact aattattttc
    60421 agcactttca agcacatcac ttatattctt cattgcatta agagaaagct ctgcaaattc
    60481 atcaaatgga atccctatat cttctatact tctcattgca tctctattta catttgaagc
    60541 aaatctcgtt tctttcattc ttttaataac agacttagtc tttacactgg taagtttttt
    60601 atctggataa actaaagcta tagcagttat aaacccagtt atagggtctg ctgcatatat
    60661 tgctttttcc attaggctag ttcttgtaac tcccattcca tcatgatgac caagtattgc
    60721 tctatacatt tcttcgtcac cgaagttttc ttttttaagc agttctactg ttgttgtagc
    60781 atgtccttca catatttctc ctggctttct tccaactata tctgaatcca aatcatgtaa
    60841 aagacctgcc actgcccact cttcttcttt ttctggctct agtcttttgg ctaattctct
    60901 cataacagct tcaacagcat aacaatgttt tctcattcta tcagtttcta aatacttaaa
    60961 taaaatttct aaagcttctt ctctcatttt caccaatccc cctgtttttt cacatatatg
    61021 atatttttat atattaatac acataaattc aagtttttat atatattcat gtatattttt
    61081 catgttatat ctattctaca cctttatagt agtgtcctgc aactgttcca atttcatcta
    61141 atttcttata caagtctgta gccttttttc caagtttata attattactc aatacctcaa
    61201 tgtgagcttc aataaccttt ttaaccatct cactatcttc aactgtaaaa tctatcctaa
    61261 atacatctat tccaatttcc tctaactcat atagattgtc caacatacaa gtaaccttcg
    61321 aattgtaaat tgtacttcta cagaatatat cttgtgttat cctataactt gcatccgtaa
    61381 agtctcttaa agcataacta ctttccttac atttagcaac tctcttgtcc tttttacaat
    61441 ctctaacaac tactcccata ggacagtatt ctgtaaccat cataggagca tatccatata
    61501 ttactgcttc aatttctaca tctgtatatt ttaaagtttg tttaatttca tttaagttta
    61561 gttcttgtga caaacaaact gtacttgctc cctcttcttt gaagtggttt atactttcac
    61621 tattgaatgc atttaagtag caatctatat ttaagtcttt tttcttaaag tattccatag
    61681 agccccagtt accaatttga accttattaa ttccttttat attatccaat atctcaaatt
    61741 gcttgtactc tttatttcta acaatccttg gtgctgaata gattaatttt ttgttagtga
    61801 ctgctgtatt atagtggttc ttattcaaag tatctatttt acttgtattt gacaaagtat
    61861 ctatgttatt tgtatttgaa cccattagtt ctttaggagt atctaaagac gaagaagtat
    61921 ctaaatccat agaaatactt gaatctatag aaatatttga atctatagaa atacttgaga
    61981 ccatttgtaa agcttttgat aatgtattta tatcttcata atagattaaa tctacattat
    62041 aatttaatac tgcttctaat tgctccaagt tcttaacttt aactctaatc ttaggaactg
    62101 tatcaagttt tttctgttta tttactctat ttggctggta ttttaatcta ttcttcttaa
    62161 attttctatc ttttatcttt attctttctt catctaaaag ctcaatacaa tcccttctca
    62221 tttgatttaa cacacttata ggcatactta caccttcatc caggtcaatt tctatctctt
    62281 ttaacttata tggtgtattt ccaagctttt gaatttgtgt ttctattttt tctttactta
    62341 aagccacctt tatagctttt tcaactattt tgtctccttc taccaccgat ttatttccac
    62401 aaaaatcact tataaaaagc tctggctttt tacctagttt aatactcact tttgcacgta
    62461 tattagtttt tatgaactct ttatcttgct gaaatgtagc ttgtactcta tccatcaatt
    62521 cgctgtcaga agtcttatat ataagctgat ttctctttgc ttctccaaca aaatctaatt
    62581 ctatcaaatc ccctttatgt gctattgtag ttatctctcc atcctttatt attcttccaa
    62641 tagtacctcc acctaagtta attccatcac ctttcttaag cgtattttct agcattactc
    62701 taagtctttt gttctttcta ttataatcta aaacttttcc aatatatagc ccttgattat
    62761 taggtctatt agagttcatt atatccttac caacttcacc taatatatat ccttttgtaa
    62821 actttctatt aaatatggta tacaaattat ttattatttc ctcagaaata cttatttttt
    62881 tcttatctac aaattcatta atcgcttctc tatatcctga aaccacagta gctacatact
    62941 ctggcttttt cattctacct tcaattttta aagataatac acctgcttca attattttat
    63001 ccaaatcttc tatagtactt aaatctctag gactcaacaa ataatctcca ttacttttta
    63061 ctacttctcc tgtatttata tctatcaact catacttttg tctacatggc tgtgcacacc
    63121 tacctctatt tccagaccta ttgcctatca tactactcat aaggcattga cccgagtaac
    63181 atacacataa agccccatgt acaaaaactt ctatatctac agttgtattt tcacaaatcc
    63241 attttatctc atcaacattt aattctcttg ctaaaacaac tctatcaagc cctacactct
    63301 gaaggtattt tacatcttct aaggaatgtg ctaccatttg agtgcttgca tgtagttcaa
    63361 agtcaggtaa tagttttttt ataagtcttg ccattcctat atcctgtact attaaagcat
    63421 caatatctat atcgtataaa aattttacat actctacaaa atcttctatt tcatactgtt
    63481 ttatcaatgt atttacagta acaaagactt gcactcccct tatatgagag tatttaacag
    63541 cctcaatcag ctcatttcta tcaaagttat ttgcagatgc tctagcacta aagtctttac
    63601 cacctaaata cacagcattt gctccgtttt gaactgctgc ttttaatgaa tcaaaagagc
    63661 ctactggtgc aagtaattct atttttttca tatttttctc ctctatttat acttgttagg
    63721 ttaatataat tatatctata acttaaatat ttgtattaaa tttatatatt tcttactata
    63781 ttttaaattc ttcaatcaat ttatatatta gttatttata tttggttcat ttgagttaac
    63841 atatacat
//
LOCUS       DAMJVI010000008        58877 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.008, whole genome shotgun sequence.
ACCESSION   DAMJVI010000008 DAMJVI010000000
VERSION     DAMJVI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58877
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.008"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            209..1285
                     /locus_tag="PC545_000620"
     CDS             209..1285
                     /locus_tag="PC545_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2455410.1"
                     /translation="MKAQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1402..2244)
                     /locus_tag="PC545_000621"
     CDS             complement(1402..2244)
                     /locus_tag="PC545_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2455411.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNETFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKVEVIDSLSGSIGIGQLVLKAAILIKEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2428..3000
                     /locus_tag="PC545_000622"
     CDS             2428..3000
                     /locus_tag="PC545_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2455412.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKDDIIYDIADTHLEVITSAILNCVKNVKSKNSQIEMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERIRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLVNHWI"
     gene            complement(3163..3435)
                     /locus_tag="PC545_000623"
     CDS             complement(3163..3435)
                     /locus_tag="PC545_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455413.1"
                     /translation="MLRERFVVRNHHNPHTSGCCGTGGEGCCGGDNHEHSHQHSHSHG
                     HEHEHVNGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDGHTHEHK"
     gene            complement(3562..4434)
                     /locus_tag="PC545_000624"
     CDS             complement(3562..4434)
                     /locus_tag="PC545_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2455414.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIIAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4721..7828)
                     /locus_tag="PC545_000625"
     CDS             complement(4721..7828)
                     /locus_tag="PC545_000625"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDF2455415.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEVYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAEKAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVIKK"
     misc_binding    complement(7921..8153)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8328..8855)
                     /locus_tag="PC545_000626"
     CDS             complement(8328..8855)
                     /locus_tag="PC545_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDF2455416.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCRENTA
                     LKEKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(8890..9675)
                     /locus_tag="PC545_000627"
     CDS             complement(8890..9675)
                     /locus_tag="PC545_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDF2455417.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITKKGKIKVQARLIV"
     gene            complement(9685..9942)
                     /locus_tag="PC545_000628"
     CDS             complement(9685..9942)
                     /locus_tag="PC545_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDF2455418.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(9961..10419)
                     /locus_tag="PC545_000629"
     CDS             complement(9961..10419)
                     /locus_tag="PC545_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDF2455419.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10459..11163)
                     /locus_tag="PC545_000630"
     CDS             complement(10459..11163)
                     /locus_tag="PC545_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDF2455420.1"
                     /translation="MGSIKDNLSEIRNKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGDKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYNKK"
     gene            complement(11173..11808)
                     /locus_tag="PC545_000631"
     CDS             complement(11173..11808)
                     /locus_tag="PC545_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455421.1"
                     /translation="MKKVKYTRLVILGILIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGTIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYHEQKLDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVIDEQSQSV
                     KFVILEGITYENESSVFVDYYKNDINGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12189..13229
                     /locus_tag="PC545_000632"
     CDS             12189..13229
                     /locus_tag="PC545_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2455422.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
     gene            complement(13692..15047)
                     /locus_tag="PC545_000633"
     CDS             complement(13692..15047)
                     /locus_tag="PC545_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HDF2455423.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDENDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VKEMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLDVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
     gene            complement(15073..15342)
                     /locus_tag="PC545_000634"
     CDS             complement(15073..15342)
                     /locus_tag="PC545_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2455424.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            complement(15632..17587)
                     /locus_tag="PC545_000635"
     CDS             complement(15632..17587)
                     /locus_tag="PC545_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455425.1"
                     /translation="MKKYEIFIFMSIIVLCFISFPHKVFGQEENCLEDFERWVQENKN
                     NKEEIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHIENGGSISIGINNIIATDGTALYVEEGGDLHIKSSYESVEKIKANGKDAI
                     GIYTENDIRILNKDIEVNGEHAIGVYSKGDVEIEETSIKAYSSNNESGLLRDNNKLAQ
                     SIISESKKVYIIDDYNELIPSIAEDSGYDIVKCCHRGIGVFSDEIKVSKDDKIENIKF
                     PKYMVLETSSGNRLDIDVEWDFTDYYEKLEKGENFNITGKFKTEMLNKEKIIINDDVV
                     PILNVSVIEKKPIDNLELEFQNTKNGYVAVLFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRVKVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEREND
                     DIDGDRGGGGRDDPDRDEDDNNQDGNNGNENNDSNNNQNNNNENNNNENDNSDEKQPN
                     TPKDDVESSTDSNENKNPHDIPKDGNKENNSDKEKGFYNKNSITENDDNYDLSSKSVF
                     GTNEHSFPYRESISNKNQDTNKNKSNISDYDGIINNNYKLESKEYNLKKDIIFLFCIL
                     TPIMIGSILIVNPDTRKSIIKFIKLKK"
     gene            complement(17770..19245)
                     /gene="spoIVA"
                     /locus_tag="PC545_000636"
     CDS             complement(17770..19245)
                     /gene="spoIVA"
                     /locus_tag="PC545_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HDF2455426.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIIQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            complement(19417..20712)
                     /locus_tag="PC545_000637"
     CDS             complement(19417..20712)
                     /locus_tag="PC545_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2455427.1"
                     /translation="MSVEVNKPSIWKNFKVLIILFVAGAFIYALPYLKNYYYDTFAQA
                     FNLTNTQMGSLGSIYGLLAMVSYLFGGFLADRISARKLLSGSLIITGISGLVLTLYPP
                     YIVVFLIHALWGITTILTFWPALVKAVRMLASENEQGKAFGFMESGRGITNAIHLSLV
                     LILFGYISSKVSDKSGLTAIIVLYSAINVIVGVLVFFKLKDNEKEVSAKFDKEAFFAV
                     IKMPHTWIITIIMFCSYSANMSFYYFTPYSTEAFGATVVFASAISILAQYCRPVASAA
                     SGLLGDKVGSSKIISYGFILMIAGLLGVIFTPTKSSMIYILLIGCVAIYVSMYAIQGL
                     HYSLLEEGNYSLSISGTAIGIVATLGYLPEVLCPLAAGKILDAFPGVTGYRYYFIVLT
                     IVAIIGLVFTFIWMNMTKERRKELIEMNNNSNENTTKVS"
     gene            complement(20896..22305)
                     /locus_tag="PC545_000638"
     CDS             complement(20896..22305)
                     /locus_tag="PC545_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2455428.1"
                     /translation="MKKKIGFWDLVFMNISALFGIRWIAKSTASSFGLGLGSIPVWLL
                     FAFIFFVPASLICAELAATYPKDGGLGEWVKQAYGEKWGFMVSWLNWTSKIFWYSSFL
                     TFLAINIAYMLGNPDLSNNKMFILALSLIIFWLLSLVSTRGMAFGKFFTNTGALGSTI
                     PAILLIVMAFMSVVILKKAPSASIYTIQNIIPKIDANSLVSISAIIFALSGAETTANF
                     ITEMDNAKKNFPKAILTVAVLIGGIYILGSVAITMILPPDKIAASTGILDALAKVAQD
                     LGIGSWFIRIVAFGITLSVLGALILYIAGPVKMLFGNVREGVFPKQLTVTNKYNIPSN
                     AVIVQAIIVSLLLVGTNLLPSVDNIYNVLVTMTALTALFPYVLLFTSYIRLRKTRPNE
                     ERPYSIAKSDSVCINIARMVLVVTVVGILFSTAPVMETLKDNIIYEIEMIGGGLFVII
                     TGLMLWKNYEKKISNNSGR"
     gene            complement(22357..23496)
                     /locus_tag="PC545_000639"
     CDS             complement(22357..23496)
                     /locus_tag="PC545_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2455429.1"
                     /translation="MSNNMRLERLRKYMNEKSIEIALIFEPDNQFYISGFKAITYSRP
                     IVTVVTQDKIELIVPGLEELHAKEVAKVDNVYVYYEIPEMKEHGISHKHYLDIILNKY
                     PKGTTVGIEKDIVSASFSEYITDKSFAIKDVGSKIFEMRYVKDAKEIEFLKIAGYLSD
                     IGIKGSLENVRVGMSELEFDVEGDNALLKYVSENYPDTYIGFANWTCSGIDRTAQPHL
                     DSNTRILQRGDIVIHSRQVWYENYRAENERTFIIGKPTERQKEVFKIAVEAQQAGLDT
                     IKAGIPARMVDEAARAVVAKYGLELYSNHRIGHGLGLSEHEEPYLRFDNELILEEGMV
                     FSMEPGIYIPGVGGFRHSDTAIVGKNGATIITNYPRSVEELILDI"
     gene            23815..24465
                     /locus_tag="PC545_000640"
     CDS             23815..24465
                     /locus_tag="PC545_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2455430.1"
                     /translation="MTNKELQKKRILMYFIEAAQNIMENEGIENITLRKVADMAGYNS
                     STLYKYFKNLDHLISFASIKYFKDYNLNISRCIENVNDEYKKYIIMWKLFCKHSFDNA
                     QAFYQVFFNLSSDELSYITKQYYDMFPEDLGIHDSDISLMITGESIKERNKILLNNLV
                     DTGYIPAKDLDIINDIIISFYQNLLFLKKNTGKNSDDDELTMKMISSIEYIIKHAK"
     gene            complement(24622..25641)
                     /locus_tag="PC545_000641"
     CDS             complement(24622..25641)
                     /locus_tag="PC545_000641"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2455431.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIVLAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTGLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="PC545_000642"
     CDS             complement(25672..26286)
                     /gene="plsY"
                     /locus_tag="PC545_000642"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDF2455432.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVAITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            complement(26321..27640)
                     /gene="der"
                     /locus_tag="PC545_000643"
     CDS             complement(26321..27640)
                     /gene="der"
                     /locus_tag="PC545_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDF2455433.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKDLTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            complement(27674..29002)
                     /locus_tag="PC545_000644"
     CDS             complement(27674..29002)
                     /locus_tag="PC545_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HDF2455434.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLDVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADTHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNDKSASKTIEQIHQIQQKYLEKLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEKILKREKKVSIATG
                     HSAYEFIQSMADAMMDKFKNLQVNVYEIKNKFFGETITVSGLLTAKDLKEQLEDKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMRIKIIPCLNEGKDFVDKILK"
     gene            complement(29240..29440)
                     /locus_tag="PC545_000645"
     CDS             complement(29240..29440)
                     /locus_tag="PC545_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455435.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            complement(29442..30374)
                     /locus_tag="PC545_000646"
     CDS             complement(29442..30374)
                     /locus_tag="PC545_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2455436.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLLSQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLVLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            complement(30419..31351)
                     /locus_tag="PC545_000647"
     CDS             complement(30419..31351)
                     /locus_tag="PC545_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HDF2455437.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYGLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNGVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKSTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            complement(31406..32695)
                     /locus_tag="PC545_000648"
     CDS             complement(31406..32695)
                     /locus_tag="PC545_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2455438.1"
                     /translation="MEDLNSIYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLRSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILQFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            complement(32724..33416)
                     /locus_tag="PC545_000649"
     CDS             complement(32724..33416)
                     /locus_tag="PC545_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455439.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="PC545_000650"
     CDS             complement(33431..34192)
                     /gene="pgeF"
                     /locus_tag="PC545_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDF2455440.1"
                     /translation="MMKDYINIKNVQDIKEYVNLSGNCEELDEKLNSVNLVITCKNID
                     AKNREDMLKVCKECDLTFENLTSNSQIHSDIVNIVNKDTIGKRRDGDALITNLEKVPL
                     LLFTADCVPISIIDAKNKAIGLAHAGWRGTFSNIGHKTIKLMSECYKTVPEDLVCIIG
                     PSIGPCCYEVSEDLVEKFNTILTNNDEKFYIIKEGSYYLDLWKINEYMLRCSGVKKEN
                     IINLNLCTSCRADKFHSYRKHNKASERIGTVLQIK"
     gene            complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="PC545_000651"
     CDS             complement(34189..34650)
                     /gene="nrdR"
                     /locus_tag="PC545_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDF2455441.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIDEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKELDEVAYVRFASVYRQFKDINTFVNELKSILIEKGDK"
     gene            complement(34672..34935)
                     /locus_tag="PC545_000652"
     CDS             complement(34672..34935)
                     /locus_tag="PC545_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2455442.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="PC545_000653"
     CDS             complement(35039..35812)
                     /gene="sigG"
                     /locus_tag="PC545_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HDF2455443.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="PC545_000654"
     CDS             complement(35904..36626)
                     /gene="sigE"
                     /locus_tag="PC545_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HDF2455444.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(36651..37523)
                     /locus_tag="PC545_000655"
     CDS             complement(36651..37523)
                     /locus_tag="PC545_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HDF2455445.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTVPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     NLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(37813..39087)
                     /locus_tag="PC545_000656"
     CDS             complement(37813..39087)
                     /locus_tag="PC545_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2455446.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGDIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEIPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="PC545_000657"
     CDS             complement(39371..40528)
                     /gene="ftsZ"
                     /locus_tag="PC545_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDF2455447.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLDTKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(40718..41071)
                     /locus_tag="PC545_000658"
     CDS             complement(40718..41071)
                     /locus_tag="PC545_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HDF2455448.1"
                     /translation="MLWVLGGLAFGVVVGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGVSLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(41170..41844)
                     /locus_tag="PC545_000659"
     CDS             complement(41170..41844)
                     /locus_tag="PC545_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2455449.1"
                     /translation="MKKKIVILGAFILGVFISTYIKSLDPNRVYITLGQTESIESEVE
                     NTKKEIKNLKETLKKNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSTVTGPG
                     ITITMRDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFAK"
     gene            complement(41849..42547)
                     /locus_tag="PC545_000660"
     CDS             complement(41849..42547)
                     /locus_tag="PC545_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2455450.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIEIYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEINSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(42570..43310)
                     /locus_tag="PC545_000661"
     CDS             complement(42570..43310)
                     /locus_tag="PC545_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HDF2455451.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNVNLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSIDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(43580..44806)
                     /gene="murG"
                     /locus_tag="PC545_000662"
     CDS             complement(43580..44806)
                     /gene="murG"
                     /locus_tag="PC545_000662"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2455452.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDIVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEDKL
                     VFTGNPVRKEILLSRKNIARKNLSISDEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMGSISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(44824..45954)
                     /gene="spoVE"
                     /locus_tag="PC545_000663"
     CDS             complement(44824..45954)
                     /gene="spoVE"
                     /locus_tag="PC545_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HDF2455453.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIEKNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGAALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(46006..47361)
                     /locus_tag="PC545_000664"
     CDS             complement(46006..47361)
                     /locus_tag="PC545_000664"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDF2455454.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASVIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDVDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSSEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKANIFMNQTVEDFCV
                     LNYDDEIVKSLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(47380..48348)
                     /locus_tag="PC545_000665"
     CDS             complement(47380..48348)
                     /locus_tag="PC545_000665"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDF2455455.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIITVVLAWISIIAVF"
     gene            complement(48389..49762)
                     /locus_tag="PC545_000666"
     CDS             complement(48389..49762)
                     /locus_tag="PC545_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDF2455456.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGDISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLNKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     TNTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDVYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(49929..51908)
                     /locus_tag="PC545_000667"
     CDS             complement(49929..51908)
                     /locus_tag="PC545_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HDF2455457.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASQIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGVEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDAKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(51920..52267)
                     /locus_tag="PC545_000668"
     CDS             complement(51920..52267)
                     /locus_tag="PC545_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455458.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGAKCILLIATFSVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(52753..53688)
                     /gene="rsmH"
                     /locus_tag="PC545_000669"
     CDS             complement(52753..53688)
                     /gene="rsmH"
                     /locus_tag="PC545_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDF2455459.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAISTAKERLSEYESRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKPIETTGELVDVIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKHLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEIEVNPRSRSAKLRVAEKI"
     gene            complement(53718..54464)
                     /locus_tag="PC545_000670"
     CDS             complement(53718..54464)
                     /locus_tag="PC545_000670"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDF2455460.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKEAKRVGIKEDD
                     VLNIAIIAIPVGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGIIAHVYLSKKNKNNISEE"
     gene            complement(54858..56144)
                     /locus_tag="PC545_000671"
     CDS             complement(54858..56144)
                     /locus_tag="PC545_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2455461.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPIGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPEGVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINENFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKEIILDYIEKSKKEGLSKEEFERTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LEYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(56159..57424)
                     /locus_tag="PC545_000672"
     CDS             complement(56159..57424)
                     /locus_tag="PC545_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2455462.1"
                     /translation="MVNTKKIINLGNNINLTLIKTEKFKSNLISLYVQRLLDEKETTK
                     NALIPSIIASGSSKYPSARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYL
                     DESIFEEVVEFFNEVINNTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCI
                     EAMCKGERYSVSEFGYEDEIDSITSRELYEHYKNILKTSPIDIVVEGNFDEDKVVDII
                     SKNLKFEREEIINIPRADFIKNVDEVKVIDEQMEITQGKLVMGYRANVDYADIDKYYA
                     LVVGSNVLGGGPHSKLFVNVREKESLCYYIFSSIEKYKSIMFISSGIETKDYDKAVEL
                     IKKQVESVKAGDISDEELENSKLALVNSMKSITDNIGGMSDFVFSQSMAKTNSEVQDI
                     ISSIEKVTKKDIVEAIKNIELDTVYFLRN"
     gene            57740..58837
                     /gene="ychF"
                     /locus_tag="PC545_000673"
     CDS             57740..58837
                     /gene="ychF"
                     /locus_tag="PC545_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDF2455463.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQTFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT        18445 a        10672 c         6337 g        23423 t
ORIGIN      
        1 aactttatag acttaattta ttacttttat agaattttca caataaaaag tcattttact
       61 tccgtattta atgcaaaagt agagattaaa cttaatataa aaaatatata acaaattttt
      121 tttatatttc tagaagaaaa gtatactctt ttttgtttaa taatgctaaa attgatttgt
      181 attgaatttt gattaggagg aaattattat gaaagctcaa agattaaatg ctgtacttga
      241 acaaatgaaa aaagatgata tttctcaaat gttagtaagt gaccctactt ctatatttta
      301 tcttacagga gtactaatcc atccaggcga aagactatta gcattatact taaatttaaa
      361 tggcaataat aaattattta taaatgaatt attcccagtt tcagaagatt taggtgttga
      421 aatggtttgg ttcaatgata ctcaaaatcc agtagaaata ataactgaac atatagacaa
      481 aaatgctact atgggtgttg ataaaaactg gccagctaga ttcttattaa acttaataga
      541 attaggtgga ggaagtaagt ttgtaaattc atcttatata atagatactt taagaatgtg
      601 taaagatgaa gaagaaaaag agttaatgag aatagcttca aaattaaatg acaaggctat
      661 ggaacaactt aaagctactg tttctggtga acttacagaa aaacaacttg ttggtaaatt
      721 atctaaaata tatgaagacc taggtactga tggattttca tttgacccta ttataggttt
      781 tggacctaat ggagctaatc cacatggtga acctggaaat gcacttgtga aaccaggaga
      841 tgcaataatc ttagatatag gttgtataaa agataactat tgtgctgata tgacaagaac
      901 tgtattctat aaagaaatac ctgaaaaagg tagagaaata tttgaaatag ttcttgaagc
      961 taataagaga gctgaagcta ttgtaaaacc aggtgttaga ttctgtgata tagatgctgc
     1021 tgctcgtgac tacataactg aaaagggata tggacagtac ttcactcata gaactggtca
     1081 ctctatagga cttgaagttc atgataaagg agatgtatct tctataaata cagatactgt
     1141 acaaccagga atgatattct ctatagagcc aggtatatat ttaccaggtg agttcggagt
     1201 tagaatagaa gatttagtac ttgttactga agatggttgt gagatattaa acaaacatga
     1261 caaagaaata tgtgtagttg gttaattcta aaatcaattt gataaaatta aaaagccata
     1321 taaaaatgtt ttaaacattt ttatatggct ttaatttctc aatctatgat ttatttcaat
     1381 ccattattta tattgcagta tttatattgc agttattaag attgcaccct ttgaagtata
     1441 tgtccccatc actgaaccaa cttcacagat tacaacatct ttgtatttta catccttttc
     1501 cataacttcc cttacctttt gagccttttc tgggcaatta gcatgtccta taaataaaac
     1561 cttttcttct ttatctttaa tttttgactc tactaaagta atcagctttt tcaaactgtt
     1621 tccttcacct cttgccttat ctataggagt tacaacgcca tctgcaactt gtattatcgc
     1681 cttaaaattt aaggtattta taatttttcc tgctattgga tttattctac cacccttaat
     1741 agcattttct aaagtttcaa gtgttccaaa aaatggagga ttatctttat atttatttat
     1801 tattgaaaca atctcttcaa tttcttttcc ttcttttatt aatattgctg cttttaacac
     1861 taattgtcct attccaattg acccactaag tgaatctata acctcaactt tcttgttatt
     1921 tccttccttt tcaaacatat cttttgctaa aagtgcacta ttataagtcc cagataatcc
     1981 tgaacttata ttaatcacta atacactttc ttcctgacaa tggtatgctt ctaaaaattt
     2041 atcaggagaa ggacatgatg tttttggtag tactttactc tcactcattt ttctatagaa
     2101 agtttcatta tctatttcct ttccagatat ataactttct tctccaaaag atacatttaa
     2161 accaacaact tctatgttgt atttttctat tatatcatca ggtaagtcac atgcactgtc
     2221 cactatcaat tttattttat ccatattaac ctcactataa gatattattt tatttattca
     2281 gtcggtcgta cgaccgattt aattattatt ttatatattg aatgaaaaaa agtcaataga
     2341 tatagattct cgctttattg acaataaata tccataagct tataatgtta ttaatttgtt
     2401 ataaatatac atggaaaggt aggttttatg aaaacaaata atatgaaaca ggctatttta
     2461 tcatctgcaa ccaatttaat agccaaaaat ggagttcaaa atactagcct tgctgatatt
     2521 gcaaaagatg taaatataag taaaggtact ttgtattatc actacgcatc taaagatgat
     2581 attatatatg atattgcgga tactcattta gaagttataa ctagtgctat actaaactgt
     2641 gtaaaaaatg tcaaatcaaa aaattctcaa atagaaatgg ttaacttaat cttagagaaa
     2701 atatctacta tagaaagtcg tggtagggta catatgtatc ttatttgtga ggcaattact
     2761 tcaaataatg atttaaagga aagaataaga ttaaaatata ttgaatggag aactactctt
     2821 aaatctgaaa ttgttgaatc actagaaaaa tataatagta gcgaaaatga acaggatgct
     2881 gaatcatttt cttttttact catgtcaata gttgatggtt tagttgtcca aagtttatta
     2941 aaaacagaaa aaatacctta tgaaaatata gcttcttttt tagttaatca ttggatttaa
     3001 acttaatact tagataatta ataaaagtga actatttaag ttatctactt taacagttca
     3061 ctttcttatt tattctcaat aatttttact taaaacattc aatttttatt taaaccttca
     3121 tggaatcaca aaataaaaat ctcttataaa tttaaaatgt ttttatttat gttcatgtgt
     3181 atgcccatct gaatgttcat gaccatgtgt atgtgtatgc tcatggctat gctcatgagt
     3241 acctgaaaaa ccatcatcat gattatggtc gtgacctaca ccatttacat gctcatgttc
     3301 atgcccatga ctgtgagaat gttgatgtga atgttcatgg ttatctcctc cacagcatcc
     3361 ttctcctcca gtcccacaac acccacttgt atgtggatta tgatgatttc ttactacaaa
     3421 tctctctctt aacataattc ttatacctcc tagtatatta ttgagtactt catgtacccc
     3481 attaactatg gtatacatct aactaggtat aatctcaata catatgtcat tttttaacat
     3541 atataattaa catatataat cttaaagaaa tttatcctta aaacctccac cttttacttc
     3601 ttcaacgtta ttgacagaaa taaaagcttc actatccata tcataaatta aatctttaaa
     3661 tttacctatt tcatttgcag atattatgca ataaatcatc tttttcttat tttgtgtata
     3721 tgcaccttct gcctccaaaa aagtgactcc tctacccatt ttattcatta tcactttgga
     3781 tatttcatca tatttatttg atattaaaag tatagatttt tttctatcaa agcaatcttt
     3841 tcctatatcc atagtaaaag aaattatata cattgtaatt aaagtgtaca tcaccaattt
     3901 agctccaaat aaaaaagctc ctgcacatac tacaaaaaaa ttaataaata aaaatgtatt
     3961 tttcataggt atatcatact ttcttttcat tatggctgct attatatcaa gtcctccagc
     4021 agtagctctt gctttaaaag ttaaccccat acctatacca gttaatgcac caccataaat
     4081 acactgtagc atagtatcat ctattgggaa atatttagca atatcttgtg ttagccccag
     4141 tgctagagaa aatagtaaca tattgacaaa acttattagc aaaaatttat tatcaaaata
     4201 ctttctagct ataataaata ttggtatgtt catcaagaaa cttgatattc cctgatttat
     4261 accaaataat ttgtataata taacacaaat tccagctact cctccactta ataacttggc
     4321 tggtgctaga tataaattta ttcctaaggt tgatagaatc atacccaata taatcagaca
     4381 atattgtaaa aatatattat tatcaatact tagcttttta ctttcaccat acatcaaaac
     4441 gcaccccttt gcataataaa taaaccaccc tgcataacac acgataaatt tacaaataaa
     4501 tttatcagaa gtagtttcct aaaaatatta ttcatcctca cttaaacgtt tattcattaa
     4561 tattctatca catgacataa ataatttata tgatttatac agatttattt attgtaaatt
     4621 ttggaatatt aaaacatttt attgaataga taattatatt acataaaaaa tccctataga
     4681 aatctataaa ctaatttcta tagggatttt gattaactaa ctattttttt ataactttta
     4741 tacctgtcat atggtcattt aagttttgtt cctctaaaga tttatcatct actctgttta
     4801 tttctaaagc taaagtttca gacttgatat attcattaaa atggtcaaca gctttagcaa
     4861 tttcatcatc accacagtat tctattacta tattatctaa tacttcaaag ttattagatt
     4921 ttcttaattg ctgtacttta gatataaatt ctctagcata accttcatta atcaactctt
     4981 cagttagctt agtatctagt ataacaaaaa gattgttttc aacagataca ttaaatcctt
     5041 ccttagcagt tatacctatt aatactaaat ccttattaaa ttcaaaagct tcaccatcta
     5101 aatcaactgt taaagtctca cctttttcaa gtttattagc agtttcatga gcatttaatt
     5161 tacttaatgc tccaacgaat aaattcatct tagaacctaa taatggccct gcttccttaa
     5221 agtttggttt taaactaaaa ttcatatact catctaaatc tttagcaaat attacctctt
     5281 taacattaag ctcttctttt attaaatcaa ctacatcact tattgtatct tcaaacttac
     5341 catctactaa cactttttgt aatggttgac gaacttttat tctttctact tctcttgaag
     5401 ctctacctaa agtaactaag tttttagcta aatccatttt ttcttctaat ttagtatcta
     5461 ctaactcttc attacattta ggatattcag ctaaatgaac agatacttct cctgttaagt
     5521 ttctatacat ttcttctgac atatatggtg caaatggagc tatagctcta caaagaccat
     5581 gtaacaattc ataagttgta ttgtacactg cttttttatc ttctgttaat tcagttgccc
     5641 aaaatcttct tcttgaacgt cttatatacc agttagataa atcatcattt ataaaatctt
     5701 gtatcattct taaagtttta tttaactcaa atatagctaa attttcttct acatctttca
     5761 ttaaattatt gaacttagat aatatccatc tatcaagttc tggtctatct ttatattcta
     5821 caaaaaattc agttggattt atatcatctg tgtttgcata taaagtaaag aagttatata
     5881 cattcttcat agtacctata aatttacttt ggatttcttt taatccatcc atatcaaatt
     5941 tagttggagt ccatggtgga gaaacatata gtaaatacca tcttaatgca tctgctccat
     6001 attggtcaaa taattccatt ggatttactg tatttcctct tgatttactc attttcttac
     6061 catctttatc aagtacaagg tctggaacta aaacactctt atatggtgct tttcctaata
     6121 caaatgttga tactgcaagt aatgagtaga accaacctct agtctggtct ataccttctg
     6181 atatatagtc agctgggaac aattggtcaa agttttcctt attttcaaat ggatagtggt
     6241 gttgagcaaa tggcatagcc ccactatcaa accaacagtc tataacctct gttactcttg
     6301 tcattggttt tccacatttt tcacatttta agtgtatatc atctacatat ggtctatgaa
     6361 gttcaactgt ttctggattt acatcttcta tagctttttc agctaattca gctcttgaac
     6421 caactgaatc tgtatgacca cattcacatc tccatatagg aagtggagtt ccccaatatc
     6481 tagttcttga tattgcccag tcatttagat tttctaacca gtttccaaat ctcttctctc
     6541 ctacaaaact tgggaaccat tttacaccat tattattttc tattaattta tctttaagtt
     6601 tagtcatctc tatataccag cttggttttg catagtatac taatggagtt tgacatctcc
     6661 agcagtgtgg ataattatga actacttttt ctttgctata taatttattt tcagaagcta
     6721 gccattttat tatttcaaca tctagcccat cttccattac aaatctacct tcccaaggag
     6781 tagatgtaaa tttaccagtt tcatctactg gttgaataac tggtaaatca tatcttttac
     6841 ctgtgttgta gtcatcttca ccaaatgctg gtgctgtatg aactatacca gttccatctt
     6901 cagtagttac ataatctcct actgtaataa aaaatgcttt tttgtcaact tctacaaaag
     6961 gcattaattg ttcatattct aaaccttcta ggtctttacc tttcatttct tctaaaactt
     7021 cataatcttc acctagtact ttatcagcta aaggtttaga tacataatat acctcatcac
     7081 cttttttaac ttttaaataa tctacatcag catttactgt taatgaaaca tttgatggta
     7141 aagtccatgg agttgttgtc catgctaaga agtattcatc agcatctttt ttcttaaatt
     7201 tagctataac tgtattagtt tttacttctt tatatccttg tgcaacctca tgactagcaa
     7261 gaccagttcc acatcttggg caataaggaa gtattttatg cccttcatat atatatcctt
     7321 ctttgttgaa tttgtttagt atccaccata cagactctat atagttatta tctaaagtta
     7381 tatatggatt atctaaatca acttcataag ccattctctc agtcattatt ctccattgtt
     7441 tttcaaatga gaaaactgac tctctacatt tttggttaaa ttcagctatt ccatattctt
     7501 ctatttgttg cttactagta agtcctaact ctttctctac ttgtatttca actggtaatc
     7561 catgtgtatc ccaaccagct tttctcttta cttgataacc agacattgtt ttatatctac
     7621 aaactgaatc tttaagcgtt ctagataaaa catggtgtac tccaggattt ccattagctg
     7681 ttggaggtcc ttcatagaat acgaaacttg ggtcatcttt ccctttttct aaagtttttt
     7741 caagtatatt tatgtctttc caataatcaa aaacttgtgc ttctgcttgt tttacagaac
     7801 tgtctaccaa tggcttaaat ttcgccatgt aaatcaccct ttcctactaa atattataat
     7861 tccaaaattt tttattcttt cacataaatt taaatactaa aaaactcgcc ccctttaaac
     7921 caaagggacg agttataaat cgcgttacca ccctaattct atatgtaaac taaatactag
     7981 ctacatataa cactctaata gtataacggt actaaccggc acagcttagt agaatttaat
     8041 cgttcagcct gcagctcaag agtgattttt atctttataa attcattggt tctcagcata
     8101 cccaattctc tgtaaaatta tctaaagata aatgtctcta tcaacgcttt taaaaattta
     8161 tctgtgttta tatgaaattc tataacaaat aatttgaata gtcaatatat ttttgaaaaa
     8221 tataataatt tctatttttc ttacattttt atcctatttt taatctttgt tatagatttc
     8281 tcaaactcac acaattattc tacataaaaa ataaacaaat tacaagctta ttctaaagtt
     8341 gtagcagctt catcattact ataattatac actgttgttc cttcaaatgc gtttacacat
     8401 ttttcatcta catcataaaa aatctcatca acacttctta tttcatcttc taacagtgat
     8461 ttaaatttat ttctaaatat cttaaattct ttgactaaag catcatactc ttttcttatc
     8521 tctataacac ggttattagc ttgttctata atttgtctag cttttaaatc agcttcttca
     8581 acaattatct tagccttttt attagccgcg ctacaagtgt cttctgctgc actttgagca
     8641 gttattaaag tagcttttag tgtttcttca atattttcat acttgtttac ttggtcttga
     8701 tataacccta atttttcttt aagagctgta ttttccctac atagagattc ataatcttcc
     8761 ttgatgatat ctaaaaattc atcaacctct tcatctctat accctcttaa acctttttta
     8821 aactctttat tttctatctc aattggagtt agcattacat ttcctcccag ctataattta
     8881 aattagtcct tatacaatta atcttgcttg aacttttatt tttccttttt tagtaatatc
     8941 tcctatttgt gttaccttag ctcttccttt gcctctaata gaaatcaaat catcattttt
     9001 tactatttta gaagttgaag ttatcttttc ataattgaca tggacttttt ctccatttat
     9061 gtattttgca ctatcttgtc tagatatatt gtaaatacca cttatcacac aatctaacct
     9121 atctgaagat actgtaaatg atacttcttt atattttgaa gaactattaa caatattttc
     9181 tctggaaatt tcctttacaa ttacattatt tcttgaaacc ttttctaagt ttacaattat
     9241 aaaatcgcaa atatcactac ttacaactac ttgacaaaag ctatcatgta taattatatc
     9301 tccaattttt tctcttttta tacctagacc cagtattgac cctaagtaat ctttatgaga
     9361 tatattttta aatttgaaat tcccttctat ttgtaaaaat ctaagagtat cttctatatc
     9421 ttcatattcc atgtaaaatg gataaataaa tacagtgctt ctttcagctt gttcataacc
     9481 tccatccaca ctgtatttta tttcatttgt agaatttaat attgctacag catttttcac
     9541 ttcatatgga ttaagaaact ccgtagactt aacatcatag ttttttatac aactattagc
     9601 cttgtctatc actttaaaca ttttattttt caagtctata tcctttatgt gatttgttag
     9661 ttttaattta tccatcctat caccttagaa tataaccaat actaattgtc ttaggaacat
     9721 caataatact attgcaatta aaggagaaaa atctataggt ccatttgtat atttatacat
     9781 tatagacctt attggccctt ctattggctc tgtaagattt tccaaaatat caaaaatttt
     9841 actttgtctt ccatttggaa accaagtcat caagcttttt ataactatca tccatgctat
     9901 tatatctaaa agataataaa gtgcaattct tattgttccc atatattaac cgccttttta
     9961 ctatttattt tgccaaggga agaatgcttt actttctaat tcttttttca tatttgcatc
    10021 tatatccaca ttattaggag ctagtataaa tattgattta gaaacctttt gtatacttcc
    10081 atctaagaca taaactgccc cattcataaa gttaaatata gatttacgaa cttcagaatt
    10141 agtcaatcct tccaaattca ctataactgc ccttctttgt tttagatggt ctgctatagt
    10201 tgcagcttca tcataaacct taggttcaac tattacaact ttcatttggc tagttgtatg
    10261 taaatttaca attttatttg cttttgcagt actaaagcca ctgttcattt cttcttcttg
    10321 aactatatcg tccatgccac tttcatattc atcttcaatg taatcttctt cttcatcaac
    10381 tatccaattc ttaaatttag atattattcc atttgacatt attaacttcc tccttaaaat
    10441 ttattcaaat ttgagtaatt attttttatt gtagtttctt tctccaaaga ttgatgttcc
    10501 aactctaaca atggtagccc cttcctctat agcaatttca taatcgtgac tcattcccat
    10561 tgacaaaaca tccatcccta cattagggat gtttagttct tttatttgtt ttgataaatc
    10621 tttaagacct ttaaatacac ctcttatctc atcttcatct tctataaaag gtgccattgt
    10681 cattagaccc tttattttta tatttttata tttttctgac acttctttga caaagtctac
    10741 aacttcttct ctatcaagtc catgcttaga ctcttcttta gatatattca cttgtactaa
    10801 gcaatctatt tctctgttta tttgttctgc acgtttttga atttcttcgc ataatgcaat
    10861 tctatctaaa gagtgtatca tacaaacttt atcgattatg tacttaactt tattggtttg
    10921 caaagttcct atcaggtgcc agtttaattt atcacctata atatcatact ttctagctaa
    10981 ttcttggggt ttattttcac caacagaagt gattccttcc ctcattgcct cattaactaa
    11041 gtctacatca acagtctttg tgactgccaa aagggtaatc tctttgctgt ctctatctac
    11101 tttttttgca gctttatcaa ttttgtttct tatctcagac aaattgtctt taatagaacc
    11161 cattattatc acctacttta tccttatatt tgtgtttata ctgttaggtc ttaaaattat
    11221 tttgtcatat agattaacac ttgtaactcc atttatgtca tttttataat aatccacaaa
    11281 tacagaagat tcattctcat aagttattcc ttctaatatg acaaatttta cactttgtga
    11341 ttgttcatca attacatata caccacgttt attgtctttt gtttttatag aagttttagg
    11401 tatttttaaa cattccatct gattatatat tatatcaaat tcttcgacac ttgtatcctg
    11461 atttgcaaca atttggttac ttatttttaa ttttaccatt attttattat tattttcata
    11521 tattttgtaa acttttgcat ctaatttttg ctcatgatat tcaattatta cattttgatt
    11581 tttttcaaag ttattagaat cttctttact gatgtatgta gatagataat tactctggtc
    11641 attggcatct ttttctatag ttccttttac atgaatcttt gcatcaactt tttcattttt
    11701 taacaccaac gtatctacat ttttgctaat tatcattact aatatattaa atactaaata
    11761 tataagaata ccaagtatca ctaatctggt atattttacc tttttcaaag actctccccc
    11821 tataaaatta ctttcattat aagtacttct tctaaaaaat gtttattcct tttttataag
    11881 ctaatatttt acgttctaat aattattttt tccatattaa atttattttt tatcaatctt
    11941 cctatacaag actgcattat ttatatataa ttgtattata aagtataagt ttaatttaca
    12001 ttttatacgt taagcttact ttatatgttt ttatagttat ttttattttt agatgcaata
    12061 taaaaccaat taagatttaa aataactttt gttatgttga catttattta ggtaatcatt
    12121 tatgaattag gataacgtta tcataacaaa agtttatgtc ctaaaaaaaa tacaaggagg
    12181 attttcttat gtcagaaatt ttattaggaa caggtttact tttattggtt ctatgttgtt
    12241 tcacaatttt tagttacaag gccccatatg gtatgaaggc tatgggagct ctagcaaacg
    12301 ctgcatgtgc tactttctta gtagaggcat tcaacctttc tttacttggt gatgttttag
    12361 gtatggaatt ttttagaagt gttggagctg ctaatggtag tcttggtgga gttgctgctg
    12421 ctatacttgt gcctttagcc ttaggtgtat ctccagttta tgcagtttta ataggattat
    12481 cttgtttaga tttttctata ttatctggat tcgttgctgg atactttgtt tctttcttaa
    12541 ttaagtttat agagaaaaaa gtgccacctg gagtggattt agtggttgta attcttctat
    12601 gtgcaccgct atctcgtctt atagcatcaa atgtagaccc attagttaat agtacactac
    12661 tacaaattgg acaagttttg atagaagcta ccaacattag ccctataata atgggaatta
    12721 tactaggagg tctaataaca gtagttggaa cgtctccact tagttcaatg gctcttacat
    12781 ctataattgg tcttactggt cttcctatgg caataggagc attaggtgct tttggttcag
    12841 cttttatgaa ctcaacacta tttgcaaaat tgaaattagg ttctaaaaaa gatattatat
    12901 ctgttgctat tgaaccatta acccaatcag acataatttc agctaatcca atacctatat
    12961 attctactaa tttctttgga ggagcctttt ctggtatcat tgtagccttg atgggtcttg
    13021 taaacatgac accaggtaca gctacacctg tggctggttt tgctataatg tttgcttata
    13081 atccagttca acaagttctt ataacagctg gtttatgtgc tttattcaat gtaattgcag
    13141 gatttttagg ttcaaaaata ttcagtacct ttaaagttct tactgctggt gaaatacgtg
    13201 gtactgatgt agaagaagaa attgcttaaa aaacaataag atttataaat ttatatctca
    13261 agtagtagag ttttatattg taggcaattt tattaattgc aaacgtttta ttttttattt
    13321 atgtttatga ttattatgtg tgttgaatat ttagataaat ttttatttat tatttttatt
    13381 tagatttttg tttagttttt tatttggttg atatattagg aataaaaatt agtatagttt
    13441 attactagtt agatagcata actttttaga tacataaaaa attttatatt aattagatat
    13501 tattaaaata aaagcgatac atgaattttt aaatgtccat gtatcgcttt atttttgcca
    13561 taatcttaac aactgatttt taaagtttat tatcttaaca aagttcatta tcttatataa
    13621 tatttaaatg catagacttc tttaatttaa ccatctaaat atttaattaa atattattat
    13681 atgtaatata actaattttt aaatgaatgt ataggagctg gaatccttcc tcctctttga
    13741 gtaaataatt cagatgaatt tgtatttata ggcattactg gtgctcgtcc taaaagtcct
    13801 ccaaattcta ccatatcacc tacatcacat cctggggctg gtataattct aacggctgta
    13861 gtcttattat taatcatacc tattgctgcc tcatcagcta tcatggctcc aagcgtacct
    13921 gctgtagtat ttcctggcac agctatcata tcaagaccta cagaacaaat agcagtcatt
    13981 gcttcaagct tatctatact taaagcacct ttaataacag catcaatcat acctgcatct
    14041 tcagatactg gtataaacgc tccactaagt cctccaacat gtgaacatgc cattactcca
    14101 ccttttttaa cagcatcatt taaaagtgct aatgctgctg tagtcccatg agtaccaaca
    14161 acttcaagac ccatttcctc taatatattt gctaccgaat caccaatagc aggtgtagga
    14221 gctagtgata agtctattat cccaaatgga acatccaatc tactagaagc ttcctttgcc
    14281 acaagttgtc caactcttgt aattttaaat gcagtctttt taactgtttc tgaaacaaca
    14341 tcaaaaggct ctccctttac tttctctaaa gctctcttaa caacaccagg gccacttaca
    14401 ccaacattta taattctatc agcctcacca acaccatgga aagctcctgc cataaatgga
    14461 ttatcttcta ctgcattaca aaatactact aattttgcac aaccaaaacc ttttgcatct
    14521 tttgttaatt ctgcagtttc ttttatgatt tctcccattt ctttcactgc atccatattt
    14581 ataccacatc ttgtagaacc tacatttact gaagcacata ctttattagt acttgctaga
    14641 gcttttggaa ttgacttaat tagtatctta tcaccttttg tatatccctt ttgaactaat
    14701 gctgagaatc caccaataaa atctatacct aaatcttctg cagccttatc caatgtctgt
    14761 gcaaacttta catagtcatt ttcatctgtt gcacctgcaa taatggaaat tggagttact
    14821 gatacccttt tatttacaat tgggatacca aattcacgct ctatatcttt tccaaccttc
    14881 accaaatctt tagcagaact cataatctta ttgtatattt tttctctagc tttatctcca
    14941 tcagggtcta tacaatctaa taaagaaatc cccatagtta tagttcttat atccaactgt
    15001 tcttcatcta tcatctttat tgtttcaagt atatttccta cattcactat tatcacctcc
    15061 ccttactgtc tactacagat tatgcattga attaaatatt tcttctcgct gaatttttac
    15121 atccactgat aatttcttac ctctttcaac caagtcctca accgttttct caaaagaatt
    15181 taatgatttc tctaaatcaa ctatcataat catagtaaaa taatcctgta atattttttg
    15241 tgatatatct actatgttga tattttgctt gtataattca tcactaattc ctgccactat
    15301 acctggttta tcctttccaa ttactgttaa aattgctttc ataataatat cctcccttat
    15361 ttttttataa atcttatatt tttacaaatt gaactatgtg ttaacatata tttcatttat
    15421 ttgttagtaa accacttttt atgtatatat ttaatatatt ttaataccta tacattcatt
    15481 tgtcaatttt ttttcaatat tctattattt ttatactatt tagaaaattc atatattata
    15541 tatgttttct caacaacaaa tacagttttc ataataatga aaggtattac ttagcaatct
    15601 catgattttc taaataatac ctttctaaaa attatttttt taattttatg aatttaatta
    15661 tactttttct agtatcagga tttacaatta atatagaacc aatcataatt ggtgttagta
    15721 tacaaaataa aaaaataata tcttttttta gattatattc tttagattct aatttataat
    15781 tattatttat tattccatca taatcactta tatttgactt atttttattt gtatcttgat
    15841 ttttgttaga aatactttcc ctataaggaa aagaatgttc attggtacca aatactgatt
    15901 ttgagcttaa atcataatta tcatcatttt ctgtaattga atttttattg taaaatcctt
    15961 tttctttatc agaattattt tctttattac cgtctttagg tatatcatga ggatttttat
    16021 tttcattgct atcagtactt gattctacat catctttagg agtatttggt tgtttctcat
    16081 cagaattatc attttcatta ttattatttt cattattatt gttttgattg ttattactat
    16141 cattattttc atttccatta ttcccatctt gattgttatc atcctcatct ctatccgggt
    16201 cgtctctacc tcctcctcca cggtcaccat caatatcatc attttcccgt tcatcatctg
    16261 gcgtctcttg attgtctccg cctcccatta taaatcctgg tttaaaaact atatttgaat
    16321 acccttcttt taatcctcct accactttta ctctaaaaca acgcaactta aaatcttcaa
    16381 aaaaaagtat agcttgatct ctaatatcct cctgttcttc agatatccaa ttgattccat
    16441 cgcttgaata ttcaacaaaa accttacttg cactatacgg catatcataa aataaaacag
    16501 ctacatatcc attttttgta ttttgaaatt ccagttccaa attgtctatt ggttttttct
    16561 ctataacaga tacattcaaa ataggaacta catcatcatt tattataatt ttttctttat
    16621 ttaacatttc tgttttaaac tttcctgtta tattaaaatt ttctcccttt tccaactttt
    16681 cataataatc agtaaaatcc cattctacat ctatatcaag tcgattacca ctagatgttt
    16741 ctaataccat atatttggga aattttatat tttctatttt atcatcttta cttactttta
    16801 tttcatcact aaatacacca attcccctat gacaacattt cacaatatca tagccagaat
    16861 cttcagctat agaaggtata agctcattat aatcatcaat tatataaact ttcttacttt
    16921 ctgatataat tgattgtgcc aacttattat tatctcttag taacccactt tcattgttgc
    16981 tactatatgc ttttattgaa gtttcttcta tctcaacatc acctttagaa taaacaccta
    17041 tagcatgttc tccattcact tcaatatcct tatttagtat tctaatatca ttttcagtat
    17101 aaattcctat tgcatctttt ccatttgcct ttattttttc aacagattcg taggatgatt
    17161 taatatgtaa gtctcctcct tcttcaacgt ataaggctgt cccatcagta gcaattatgt
    17221 tgtttatacc aatacttata cttccaccat tttctatatg tattactcct tcttttcctc
    17281 cttcacctat tatattcaat tcattatcat caataataaa tctcccatga ttttttatca
    17341 atattttata tttgttggta tctattgtaa aattactgtc ataaggaata taaaatctaa
    17401 actcttcatc tattaccata tcacaagaaa gagtataaac tatttcttct ttattatttt
    17461 tattttcttg aacccacctt tcaaaatctt caagacaatt ttcctcttgt ccaaaaactt
    17521 tatgtggaaa acttataaaa cacaaaacta ttatactcat aaaaataaat atctcatact
    17581 ttttcatctt acactcctag aaatttttag ctttgaaaca atcctgtcga aacatatact
    17641 tcacccacta taatttttcc acaattatca attattgtca atttatataa gtatctttat
    17701 taattgttat aaataaaaag ctacttacta aagcaaacag acgtttcact ttaataagta
    17761 gctatatttt tataacaaaa tagttataat attagaactt ccttcattta caattttttg
    17821 taaagtcttt tgcattttaa ctcttatctc ttctggcata gtatataatt tactttgtaa
    17881 ttgctctttt actaagtcat gtagtgattt accaaacata tttgattccc ataaatctgc
    17941 tggttgctct tcaaatactt ccataagata ttttatcatc tcttcacctt gattttgatt
    18001 tccaactatt ggagaaacct ctgtagatat atctgctttt ataatatgaa gtgatggtgc
    18061 attggctttt aacttgatac catattgttt tccttgtttc attatttctg gttcttccag
    18121 actaagttct tctaaggaag gagctactac tccatatcct tttatcttgg catctattaa
    18181 agcactttct attttatcat actcattttt aactttagat agtctagtta tcagactaag
    18241 cagttgataa tctccttcaa ttttaaatcc acttttttct tcaaggacat tatagaataa
    18301 atcttgcttt gtctgtaaat caatatttat aacaccttct cctagctcta cattatcaac
    18361 acctgtatct tccaaaaact ctaactcaga aaatccttgt ataatacctt ctatatccct
    18421 tattttacct atatctataa tactttgttt taatgttgtt attatgctac tttttatcca
    18481 atgatttctt tcaagacctt caacccattt tggtaagttg attcttattt ctgtaagtgg
    18541 aaaatcatat aatacagctt ccataacctc ctctatatct tcttcttcca tttcaaccac
    18601 attcattgga agaactggaa cttcatactt ttcttccaat tcacttctaa gcatactcgt
    18661 ttcctcactt tttggactta acgtatttaa aaccactgca aatggttttt taagattttt
    18721 caattcttgt ataactctct cttctggttc tacatagctt tttctgtcaa ttccagtaac
    18781 tgaaccatct gtcaagacaa ctatccctat tgtagaatgg tcttttataa cttttttagt
    18841 tcctatctct gctgcctttt caaatgtcat agcttcttgt gaccaaggag tggatactaa
    18901 tctttgtttt ccaccttcct catgccctaa agctccttca actatgtatc caacacaatc
    18961 taccattctt acttttaaag atactgtgtc ttttattttt atttctacac catctgctgg
    19021 tacaaatttt ggttccactg tcattatcgt tttacctgaa ccactttggg gtatctcatc
    19081 tcttgttcta tcctttttaa attcattgtc tatattaggt ataaccaact tttccataaa
    19141 ttttcttata aaagttgatt ttcctgttct tacaggtcca actactccaa tatatatatc
    19201 cccctgagtc cttttggata tatcttcgta tatgttatta ttcatattat tcctcctata
    19261 catctaatct tattaaatta tatttagagt taagggttaa tattcttaaa atagaaaaat
    19321 aaagaccagc aatattaatt taatttacat atagaaattt gtaaatttca atgtaaatta
    19381 aattaatatt gctggtccaa atagtagtgt attatattaa ctgactttag tagtattttc
    19441 atttgaatta ttattcatct caataagttc tttacgtctt tcttttgtca tgttcatcca
    19501 tataaatgta aacaccaatc caattatggc tactatagta agaactatga agtagtacct
    19561 atatccagtt acacccggaa atgcatccaa tatcttccct gctgctaaag gacaaagtac
    19621 ttctggaaga tatcctaatg tagcaactat tcctatagca gttcctgata ttgataatga
    19681 ataattacct tcttcaagta acgaatagtg taaaccttga attgcataca tagatacata
    19741 tattgcaaca catccaatca atagtatata aatcatagat gattttgttg gtgtaaatat
    19801 tacacctaaa agacctgcaa tcataagtat aaatccataa gatataatct ttgatgaacc
    19861 aactttatct ccaagtaatc cagaagcagc tgatgctact ggtctacaat actgagctaa
    19921 tattgagatt gcagatgcaa acaccacagt agctccaaat gcttcagttg aataaggcgt
    19981 aaaataataa aatgacatat tagctgaata tgaacaaaac attattattg ttattatcca
    20041 tgtatgaggc atttttatta ctgcaaaaaa ggcttcttta tcaaattttg ctgatacctc
    20101 tttttcatta tcctttaatt tgaaaaatac taatactcca actattacat ttattgccga
    20161 gtataatact ataatagctg taagaccaga tttatcactt acttttgatg aaatataccc
    20221 aaatagtatc aatactagag atagatgaat tgcattggta attccacgac cactttccat
    20281 aaatccaaaa gcttttcctt gttcattttc agatgctaac attctaacag cttttacaag
    20341 agctggccaa aaagtaagta ttgttgttat tccccataag gcatggatta aaaacacaac
    20401 aatatatgga ggataaagtg ttaatacaag accagatatt cctgtaataa ttaaagaacc
    20461 tgaaagcaat tttctggctg aaatacggtc tgctaaaaaa ccaccaaaaa ggtatgatac
    20521 cattgcaagt agaccataaa tacttcctaa gcttcccatt tgtgtatttg taagattaaa
    20581 tgcttgtgca aaagtatcat aataataatt ctttaaatat ggtaaagcat atataaatgc
    20641 tcctgctaca aagagtatta tcaagacttt gaaatttttc caaatgcttg gtttatttac
    20701 ttcaactgac atacttcctc cttatgtaaa tacctgtaaa ttttcatgat aattaattat
    20761 tgcttattgt attgtttatt caatatttcg gaatatttat taatatatta ggttaatcta
    20821 tcaataattt atggtgagtt atgagctaaa agtaaactct tttagctcac cataataatt
    20881 atatttttat ttatactaac gtccagagtt attagatatc tttttttcat agttcttcca
    20941 tagcattaaa cctgttatta taacaaaaag acctcctcct atcatttcaa tctcatagat
    21001 tatattatct tttaaagtct ccataactgg tgccgttgag aaaagtatgc caactacagt
    21061 tactacaagt accattctgg caatattgat acaaactgaa tcactttttg caattgaata
    21121 tggtctttct tcatttggtc ttgtctttct aagtctaata taagatgtaa aaagcaagac
    21181 atatggaaat aatgctgtta atgctgtcat agtaacaaga acattgtata tattatctac
    21241 tgatggtaat aagtttgttc ctacaagaag tagactcact attatagcct gaactattac
    21301 tgcatttgat ggtatattgt atttatttgt aacagtcaat tgcttaggaa atacaccttc
    21361 tcttacattt ccaaaaagca tctttactgg acctgctata taaagtatta atgctcctag
    21421 tactgataaa gttattccaa atgctactat tcttataaac caacttccta tacctaaatc
    21481 ttgtgcaact ttagctaatg catctagtat ccctgtagat gctgcaattt tatctggagg
    21541 aagtatcatt gttattgcta ctgaacctaa gatatagatt cctcctatta aaacagccac
    21601 agttaatata gctttaggaa agtttttctt agcattatcc atttcggtta tgaaattagc
    21661 agtcgtttca gctcctgata atgcaaatat tatagcagat atagaaacta atgaattggc
    21721 atctattttt ggtattatat tttggattgt atatatcgat gctgatggag cttttttgag
    21781 aataactaca gacataaatg ccatcactat aagtaatata gcaggaattg tagaacctaa
    21841 tgctcctgta ttcgtaaaaa attttccaaa tgccatccca cgagtactta ctaaggacaa
    21901 cagccaaaat attatgagtg aaagtgctaa aataaacatc ttattgtttg ataaatctgg
    21961 atttccaagc atatatgcaa tatttatagc taaaaatgtc aagaaagaag aataccaaaa
    22021 tatctttgat gtccaattta gccatgacac cataaatccc cacttctctc cataagcttg
    22081 tttaacccat tctccaagtc caccatcttt tggatatgtt gcagccaact ctgcacatat
    22141 aagtgatgca ggaacaaaga atataaaagc aaacaataac caaactggta tagaaccaag
    22201 acccaatcca aaactagagg cagtagattt agctatccat ctaatcccaa atagtgctga
    22261 tatattcata aatactaaat cccaaaatcc aattttcttt ttcactgtaa ttcctccaat
    22321 ttcaaattta ttcaaactca tacactatgt attgatttaa atatcaagta taagttcttc
    22381 aacacttctt ggataattag ttataattgt agcaccattc ttacctacta ttgctgtatc
    22441 tgagtgtcta aatcctccaa ctccaggtat atatatccct ggttccatag aaaataccat
    22501 tccttcttct aatattaatt cattgtcaaa tcttaaatat ggctcttcat gttctgacaa
    22561 acctaaacca tgaccaattc tatgatttga atataactct agaccatact ttgcaacaac
    22621 agctcttgct gcttcatcca ccattcttgc tggtatacca gcttttattg tatctaatcc
    22681 agcttgttga gcctctactg caatcttaaa cacttctttt tgtctttctg ttggtttgcc
    22741 tataataaaa gttctttcat tttctgctct atagttttca taccaaacct gtctactatg
    22801 tattactata tctcctcttt gaagtattct agtattagaa tctaaatgag gttgtgctgt
    22861 tctatcaatc cctgaacaag tccagtttgc aaatcctata tatgtatcag gataattttc
    22921 agaaacatat tttagtaaag cattatcccc ttcaacgtca aactccaatt cactcatccc
    22981 aactctaaca ttttctaaag acccttttat tcctatatca gataggtaac ctgctatctt
    23041 caaaaattct atttcttttg catcttttac atatctcatt tcaaatattt tacttccaac
    23101 atctttgata gcaaatgact tatcagttat atactctgaa aagcttgcag atactatatc
    23161 tttttctatt ccaacagttg ttcctttagg gtatttattt aaaattatat ctaagtaatg
    23221 tttatgtgat attccatgtt ctttcatttc tggtatttca taatacacat atacattatc
    23281 aacttttgca acttctttag catgtaattc ttctaatcca ggtactatta attctatttt
    23341 atcttgagtt actactgtta caattggtct agaataagtt attgctttaa atccactaat
    23401 ataaaattga ttgtctggtt caaatattaa agcaatttca atgctttttt cattcatgta
    23461 ctttctcaat ctttctagtc tcatattatt tgacataatg cacctctttc ttaatattca
    23521 gataatttat tcaatacaac aaggatgaat atattatttt aaaattctta atatttttag
    23581 tacttgtcgt atttgtatgt aaattaattt tatttgtgat tattttatca taatcatgat
    23641 tattttgtca atcatgattt tattttaaaa aattttttat attctagtga ttttaaatta
    23701 tctaaaatat aacgagtata cattgtattt tgtaatatta ttcttgattt atggtaagtt
    23761 taatgataaa atcacgatta tataaactat attgattatt tgtaggtgaa taaaatgaca
    23821 aataaagaat tacaaaagaa aaggatattg atgtatttca tagaagctgc tcaaaatatt
    23881 atggaaaacg aaggtataga aaatattaca cttcgtaagg ttgcagatat ggctggctat
    23941 aatagttcaa ctctatataa atactttaaa aatttagacc atttaatatc atttgcatct
    24001 ataaaatatt ttaaagatta taacttgaat atttcaagat gtatagaaaa tgtcaatgat
    24061 gaatataaga aatatataat tatgtggaaa ttattttgta agcattcttt tgataatgct
    24121 caagcttttt accaggtatt ttttaacttg tctagtgatg aacttagcta tataacaaaa
    24181 caatattatg atatgtttcc agaagattta ggtattcatg atagtgacat atctttaatg
    24241 attacaggag aaagtataaa ggaaagaaat aagatactat taaacaatct tgtagatact
    24301 ggatatatac cagctaaaga tttagacata atcaatgata taattatttc tttttatcaa
    24361 aatctattat tcttaaaaaa gaacacagga aaaaattctg atgatgatga attaactatg
    24421 aaaatgattt cttctattga atacataata aaacatgcta aataaaatta aaatatggct
    24481 gtctcaaaat agagataagc cttttttatt gttgaaataa taaaaatata tttaatttca
    24541 tataaaaatg agtgcctcgt aaactaaaaa gttcattttg agacacccct aaaataaatt
    24601 tattatttat tatacaatag tttatatatc atcacctaaa actacttctt ccatttcatg
    24661 ctttttactt ctcatcatta gttctattcc aacttcttta ggatttgaac ctttctttat
    24721 tacagaatat atagcattag ttataggcat atctatatct aatttttcag caacatcatg
    24781 tgctacttca gtagcagtta tgccttctac aaccatcttt acttctttta aagtatcttc
    24841 taaactcatc cctttaccta tgagtatacc agctcttcta tttctactat gcatacttgt
    24901 acaagtaact attaaatctc caatacctga aagacctgca aaggtagaca tattagctcc
    24961 catagcaatt ccaagtctgc tcatttcact aatacctctt gtcataaggg cagcttttgc
    25021 attatcacca taccctaatc cgtcacatat cccagctcca aatgctataa tattttttaa
    25081 tgctcctcca agttctactc caactatgtc tggattcgta taaactctaa gtttagggct
    25141 cataaacaaa tcttgtatca tctgtgctat ttttaaatct ttagaagcaa ctactacggt
    25201 agtaggaata tcccttgcta cttcttcagc atgagatggc ccagatagta ttacatatgg
    25261 attttgtggt aattcttctt cacagacttg tgataatcta agcccagtcc ctttttcaag
    25321 acctttagct acattaacaa taacttgtcc ttcttttata aatggtttta ttttttggca
    25381 agtacttctt acagcctgtg atggaactgc taaaactatt accttacaat cttttatagt
    25441 tttttctata tcagtactta tagttatatt atttggaaat aatactcctg gtaaatagtc
    25501 tgtattttct ttagttctgt ttattttttt agcttgttcc tcttttcgag tccacatgct
    25561 cacatcatta cctttttttg caagccctaa agctaatgca cttccccagc ttccagtacc
    25621 taatacacat actttctcca tgtttaacac cacctgagta taatatattt actattcttt
    25681 cttttgacca atttttctct ctgtcccatt taataatcgc tttatatttg ctctgtgatt
    25741 atatattact gatgcagtta agaacagagc tactattaaa ccagctttat tttttaccat
    25801 aatcataaat attggtgaac atcctattcc tacaacagaa cccaatgaaa catactttgt
    25861 tatagctacc accaatataa atactgctag acacattagt gttattactg gatttacagc
    25921 tagcattgcc cctagtgaag ttgcaacacc tttgcctcct ctaaatccta aaaccgctgg
    25981 ccaattatga cctgctacaa cacatataac tgccagatat ccagctaaaa gagtatctat
    26041 tccaaccaat cttgctgcaa attctgatat taaaacagcc attacacctt taagaacatc
    26101 acctaaaaat gtcattgctc ctgctctttt tccaagtgtc ctaagtacat ttgtcgaacc
    26161 tgcattacca gaaccttgag tccttatatc aaccccagca attcgctttg caactatgta
    26221 tgaggtagat atatttccta aaagatatgc aactacagca ataattatat aactaaatat
    26281 ctccactgaa tttcccccct ttagttatat tttaagatat ttatttttta tttttttgtc
    26341 tatactcaaa ctttatggaa gttccttcaa atccaaaatt ttctcttatc ttattctcaa
    26401 ggtatctttg atatgagaag tgagttaaat ccttatcatt tataaacaat gtaattttag
    26461 gaggtctaat gtcagtttga gtcccatagt aaatttttaa tcttctacct ttgtcagatg
    26521 gtggttgatt taacattaca gcttctccaa ttacttcatt cagtgctgaa gttgatattc
    26581 tcttagaatg ttcatttgac acatattcaa ctgtatctag tatcttgttc attctttgat
    26641 tagtttttgc agaaacaaat acgattggag catacatcat aaatggaaat ttttctttta
    26701 tatcttttgt gtaattactc atagtcttat tatctttctc tattaaatcc catttattga
    26761 ttacaaatat gcatcctttt ccttcatcat gtgctatacc tgcaaccttt gtatcttgtt
    26821 ctgttacacc ttctaaggca tctattacta tcaatactac atctgctcta tctacagcac
    26881 tcatagctct tattacactg tatttttcta ttgtctcata tattttactt tttcttctaa
    26941 gccctgctgt atctattaga agaaatttct gtccattttt ttcaaaataa gtatcaattg
    27001 cgtctctagt agttcctgca attggactta caattactct ttcttctcca agtatcttat
    27061 tgagtataga actttttcct gcatttggtt tacctgttat agctactctt attatatctt
    27121 cttcatactc tgtatctaat cctgctggaa aattttcaac tatctcatct aataaatctc
    27181 caaatcccat actattggct cctgaaactg caaaaggcgt tccaaatccc aactcataaa
    27241 aatcgtatat attatcaaat tgactttgac tatcaatctt attaaccact aagattactg
    27301 gtttttttgt ctttctaagc aattgtgcaa tttctttatc agcagctgtt attccagctt
    27361 taccatcaac tataaaaagt attacatgtg acatgtccat tgcaagcatg gcttgatttc
    27421 tcatttgaga taatattata tcttcgctat caggttcaat tcctcctgta tccactaatg
    27481 taaaatattt atctaaccat tctacttcag caaatattct atctcttgta actccaggtg
    27541 tattttcaac tattgatatc ctttttcctg caaatttatt aaatattgta gacttaccaa
    27601 cgtttggtct cccaactaca gctacaactg gcctacttat actcatttta tttctccttc
    27661 catcttttgt tttctatttt aaaattttat ccacaaaatc ttttccttca ttcaaacaag
    27721 ggataatttt tattctcata agttcttcta attgatttaa ttcaatatta tctaagaaaa
    27781 tttcttcatc tgcttttagc atacttcttg ttatataaag agcttctcct aattctttat
    27841 cttcaagttg ttcttttaaa tccttagctg ttaaaagacc tgatacagtt attgtctctc
    27901 caaaaaactt gtttttgatt tcatatacat ttacttgaag atttttaaac ttgtccatca
    27961 tagcatctgc catactctgt ataaattcat atgcagaatg accagtagct attgatactt
    28021 tcttctctct ttttagaatc ttctcaggca atatatttag atactcttca atctctgttt
    28081 ttaacttacg tatcatacct acaccatctt caaactgtaa aaaaccttca tattcttcat
    28141 atccaggcaa ttcactatta gatagtatat aaaattcatc tgataaaaat gcaaatcgag
    28201 tacctaattt ttcaagatat ttctgttgta tttgatgtat ttgctctata gttttacttg
    28261 cgctcttatc attaaatata tttaattcta ctagattttc tctatgcttt gtaatcccaa
    28321 ctggaactat cgcaacactg tttacatacg gataaagttg agctagttct tttattgttc
    28381 tatccaattc ctttccatca tttatacctg gacataaaac aatttgacag ttcatttcta
    28441 tatgagtatc tgccaatctc ttcattatac catataattt tcctgcaaat ttattgctta
    28501 tcattttttg tcttaactct ggattagttg tatgcacaga tatatttata ggacttattc
    28561 tatattttat aatattattt atatcttctt cactcatatt tgtaagtgtg acaaaatttc
    28621 cttgtaaaaa agataatctc gaatcatcat ctttaaaata aagagtttct ctcatacctt
    28681 ctggcaattg gtctataaaa caaaacatac acttatttct acagctttta gcttggtcaa
    28741 ttatcggatt tgtaaattca acacccaaat cttcatcata gtctttttct atctcgtata
    28801 tgtagacttc gccatctttt ttttgtattt ctacatctaa atattcatca cttagtaaga
    28861 atctatactc tattatatca tgtattggct gttcattcac agatataagt aaatctccta
    28921 cctctatacc tatttcttct gcaatactat ctttataaac cttacttata atattattta
    28981 ctttttttac atttacttcc aatattttca ccactctttt gtattcatca atgcttatta
    29041 taatatattt taatatgcaa tttatttatt tattatattt tatgatagca tttttaagca
    29101 tttcatgtca taataacaca ttttaattct aatagtcaat ataagtaaat agctataaaa
    29161 tagattagaa caaataatat tcaaatttaa gtttcttaaa aatttgaata ttatttgttc
    29221 ttaaaaaaat tttataaatt taatgtttta ctatgattaa cgtcccattt gtcatatcat
    29281 gaaccattcc acctacacac tttgacatca acaatgcctt attttgtaag tctttctcat
    29341 catttgcata aaatattgga caacctccac catttttcat attcttatca agtgtaacta
    29401 tagctagggt atattcattt aaacctatat ccattcccat cttatgcact tcctttcttt
    29461 cctattttat aattttttag tgataagttt tttcctttag aacttgaaag tattgggcat
    29521 gactttacgg cttccattac tttttccaca ttcttatcta ctggtaaata agcaatcatt
    29581 atactttcat cctctttatt ccttctaggt aatggtacga atgaaggttc tccataatct
    29641 ccatataaac ctaaccttga atatatattg tatactattg cctgtctttg acctgagtca
    29701 aataatatcc ctgcattagc ataatcttta tttttaggaa ttatttgtat tcctacacct
    29761 ctattaatat atacttctct atcacttttt agacctatat ttgttattcc ctttaaatct
    29821 cctaccttca atatagactc atctacaaaa ctaatctcag caggcattac atcagctatg
    29881 tctccaatac tttttctagt aagaagtttt tttaatataa aggctaacac taatccacaa
    29941 attgaactta ttaccaaact cattccttca cttattttcg tttctgcaag tattacataa
    30001 tacaatccta ttgttaaaaa agatgtaact atacacatat aatttcttac ttcataggtt
    30061 ctagctatct cttctatgaa tgcagctcct ctctttacaa gttgaacttc ctctaagttt
    30121 tgcaatgtat ttcttctatt tgctctcact tgtctaaatt gctctgctgc tagggataaa
    30181 aaagttatag atgtataaga acgatttata agagctggaa ccaataatgc tcctaatgaa
    30241 gaagcgacga atgctaatat aagttgagat agtaaaacat ttggttgcgt tggatactgt
    30301 ttttggtcta aattcaacat tataattcta gatgttatac caataataac agcaacaaaa
    30361 aatactctat ccattaaatc actcctaaaa tatttaattt gaaattttac atgtaaatct
    30421 acaaagtctt tctagaaaaa tcactctgct ttccttttga tgtttctatg ataggtgtac
    30481 ttttcactac atctatcatg acatctatat ctttgagtat tggcatatat ggtataacca
    30541 cagtactttt ttctaaattg gtcttagata tagcaacaat atcaacctca tctacatctc
    30601 tatctatccc catatgaata aatagattat gtattatagc ttgtctttgc cctatatcat
    30661 ttactattcc aaatgcacct aaatctcttg gtataacttc tatggccaga cattcatttc
    30721 tatacttttc tcttgtactt tctagaccta tgttagttat tattacacca tttaccatta
    30781 aaataggtcc ttcaaaactt atttttgcag gaactatatc tgctatatct gctatactgt
    30841 tccttcttaa aaatcttcta aaaatcaaac caacaatagc tcctgacaca atagctagga
    30901 ttgtgcaata aaaaaagcta aggccatact ttcttgcaaa aacgatataa actatcgaag
    30961 ataccagtgc agaaaaaaga cttatataac ttctagattc atacgtagat gctatttctt
    31021 caacgtaagc gtctcctttt ggtactaatt cttcattatc gatattcttt agtgttattc
    31081 tttcttgttc tgcaagacct tgaaactgtt gtattgctac tgcaaaaaat gtcaaggctg
    31141 caaactcctt atctataagt gcaggtaatg ctatagcacc taacgaagct gaaagcccag
    31201 atataattat ctgttctaaa taatcttgtg gtcttgatgg gtattgtttg tctgtaactc
    31261 ttaaaactaa tcctctacat aatattccta caactaatgc aactataaaa gagtgtctaa
    31321 ataaatttgc attcaataaa ctttcttcca ttttctcagc tccttctctt atatagtttg
    31381 tgcaaaaaac aaaaaaaatt cccttctatt taaggaaatt tttttgtaat attgtaacta
    31441 taaattcgct accaatgcca agttcacttt ttacttttat acatccattt agtgatttga
    31501 ctatatgctt tgttatagcc aatcctaaac ccgttcctcc tacatcccta cttcttgctt
    31561 tatctaccct ataaaatcgt tcaaatatgc gttctacatc ttcttttggt atccctacac
    31621 cattgtctat aactttaatt acgatttttt cttcatcttt taacactaca acttgtacaa
    31681 tcccattatc ttgggtatac tttatagcat tatcaactag gtttaagaac acttgcttta
    31741 tatagtcttt attggatagt atacttattg atttatcatc acatttatat aaaacttcta
    31801 tatttttttc actagctgaa tgttgagtta tttcatatac ctctttaaat acatcatata
    31861 ctacaacatt ttccataaga agagtatctt tattttctac aaatgataac agcaatatat
    31921 catcaatcaa cctttttaat ctatctgatt cgctttctat tatttctaag aatctatttc
    31981 tagtattcac atctatattt tcatttgact ttagtgtttc aacaaaccca cttatagatg
    32041 taagtggtgt tttaagttca tgacttacat ttgcaacaaa atcacttctc atattttcaa
    32101 gtttaacttt tttagtaata tcttctatat ttactatcga acctattatt acgttgttag
    32161 tattctgaag ataaactggg tctactttta tcttatatac gatatcatct ttagtagtaa
    32221 tattagcagt ttcattatct ttagaaccaa taaattgaag tatttgtttt aatatttctt
    32281 cttcttttat tacaacatta atattatgac cttcaactaa ttgatttggt tcactcttta
    32341 tcatttcttt tgcttcatca tttatcagaa gtatatttcc atttacatct atagctaaaa
    32401 ttccatgaga tatactcttt aatatagacc ttagctgtaa atgtttatac tgtacttctt
    32461 ctaaggtttt atccatattc tttatcatat gattaaaatt tctagctaat tcgccaagtt
    32521 ctcctttggc tgatatgtta agcctagcat gatattcatt attacttact ttttttgaga
    32581 cgtatataaa ctcttttaaa tatttcctta atgttattgt gtatcttaca gctaatataa
    32641 caatggctat ggacaacata acaataaaaa aatatatact atttaaatct tccacaagat
    32701 aacactccta aaatataacc tttttaatct attttgtatc ctacacctct tatagtctct
    32761 atatattttt ccgatttatc ttcatcttct attttttttc ttaaatatct tatatgaaca
    32821 tctactgttc tagtttctcc atagtattca tacccccata ttttatccaa aagataattt
    32881 cttgaaagta cttttccttt attttgaatt aataatttta acaattcaaa ttccttaaga
    32941 gttaactcta tcttttcaga gcctttggta acttcatgtt ttgataaatc tagttttaaa
    33001 ttacctgtag taagtatatt gttttcttca cctaagctag tcaaattata tctccttaaa
    33061 acagaactaa tcctagctaa caactctttt atactaaatg gcttagttat atagtcatca
    33121 gcacctattt caagcccttc aaccttgtca ttttccatat ttttagcagt aagcattata
    33181 acaggaatat tttttaagtc cttatcactt cttatttttc tcaaaacttc tattccactt
    33241 atatttggta acatccaatc taaaagtatt aaatctggtt taatttcctt agcctttata
    33301 aatccgtcaa atccatcata tgaataacta acttcgtaat ttgacacctc caaattgaat
    33361 tttaatagct caactatatg catttcatcg tctataacaa gcactttagt attcattaaa
    33421 aagctcctct ctattttatc tgtagtaccg ttcctattcg ttctgatgcc ttattatgtt
    33481 ttctataaga gtggaattta tctgctctac aactagtgca taaatttaaa tttattatat
    33541 tttctttttt aactccacta catctaagca tatactcatt aattttccat aaatctaaat
    33601 agtaacttcc ctcttttatt atatagaact tttcatcatt atttgtaaga attgtgttaa
    33661 atttttctac taaatcttca gacacttcat aacaacatgg tccaattgat ggcccaatta
    33721 tacatactaa atcttcagga actgttttat agcattcaga cattaatttt attgttttat
    33781 gacctatatt actaaaagtc cctctccaac ctgcatgagc cagtcctatg gctttatttt
    33841 tagcatctat aattgatata ggtacacagt ctgctgtaaa cagtaaaagt ggtacttttt
    33901 ccaagtttgt tataagtgca tctccatctc ttcttttccc tatagtatct ttattgacta
    33961 tattaactat atctgaatgt atttgagaat tactagttag attttcaaat gtcaaatcac
    34021 attctttaca tacttttaac atatcttctc tattttttgc atctatattc ttacatgtta
    34081 taactagatt tacagaattt aatttttcat caagttcttc acaattacca cttaaattaa
    34141 catactcttt aatatcttgt acatttttta tatttatata atctttcatc atttgtctcc
    34201 tttttctatc agtatacttt taagttcatt tacaaaagta tttatatctt taaattgtct
    34261 atacacagaa gcaaatctta cataggcaac ctcatctaac tctttcaatt tatccattac
    34321 catctcaccg atttttttgg tttcaatttc ttctataaaa catttattta tttcattttc
    34381 aatatgtaca acaatacttt caatctcgtc aatagaaaca ggtctttttt cacatgcttt
    34441 tagcaatcca tattttatct tttctctatc aaaatattct ctactattat cctttttaat
    34501 gaccattaaa ggagttgttt ctattttctc atacgtagta aacctctttt tacatgattc
    34561 acactcacgt cttcttctga tagattttaa atctgtatgt ctagaatcta ttacctttga
    34621 ttctttataa ttacaataag gacattgcat ctatattcct cctttgttta actatatctc
    34681 aaactcatct aagcttaatt ctgaacctat atttactatt attgtatcac aacctatttt
    34741 aagtatatca ttccaaaata taacttcttc atcccctcct cttgaaaaaa aacttctact
    34801 tccatctcca aaaatggtaa atgagactac cttaccttcg tgaatatcca tgtctatgtc
    34861 tattataaac cccatccttt ttccattttt tacatttata atttcttttt ccattatatc
    34921 agaaactcta atcatattgt actcaccctt tctttaaaaa taaatatgta tcgaagtaaa
    34981 aaaaaatgac ctatataggt catttaaatt aaaataatgt ttacagtcca tggattactt
    35041 atacatattt tctcatattc tttaaagcat ttttttcaat tcttgatact tgtgcttgag
    35101 atataccgat ttcatcagca acttctatct gagttcttcc tttgtaaaac cttaaatcta
    35161 ataccaactt ttctctacta tttaatttct taatagcttc tttaagtgat atttcttgca
    35221 accaattttc atcagtatct tttttatctt gaacctgatc cataacgaat attgcatctc
    35281 cattgtcttg atatactggg tcaaacagag atattgggtc ttgtatggca tcaagtgcca
    35341 ttacaactga ttctacttct aattctaatt cctttgctat ttctgatact gtaggttctt
    35401 tagaatttgt tctaattaat ctttctctaa cttgtagtgc cttatatgct atatctttta
    35461 atgacctact tactctaatt gggttattgt ctcttagata ccttctaatt tctcctataa
    35521 tcataggaac agcataagtt gaaaacctta cattttggct taaatcaaag ttatctatcg
    35581 cttttataag accaatacaa cctatttgaa ataaatcgtc tatattttct cctctgttgt
    35641 taaacttttt tattacactc aagactaatc ttaaattccc tcttacaaac tgttgtctgg
    35701 cttcttcatc accatttttt atttgtaata aaagttcctt catttgctta tttttaagaa
    35761 ctggaagttc tgatgtatta acaccacata tttcaacctt attaacttgc atatattttc
    35821 agtcctctct caaaagtatt catctattga aattatttac atttccaatt tttttatact
    35881 ttttaagaag aattaattta gcattataca aattttttca tttctttttg caaccttgag
    35941 attattttct tttcaagtct agatatgtag gactgtgata ttccaagcat tccagcaact
    36001 tctttttgtg ttttttcaat acctttatca ataagaccaa atcttaattc cataatttgc
    36061 ttttctctgt cacttaatct gtcaagtgcc ataaccaaca aatctctatc tatttcttct
    36121 tcaattattt tgtatatttc atcattttct gtacccaata catcagataa taaaagttcg
    36181 tttccatcta aatctatatt taaaggttca tcaaatgata cttctgtttt ctttttatta
    36241 ttttttctta agtacattaa tatttcattt tctatacatc tcgaagcata agttgccaat
    36301 tttatatttt tattcagttt aaaagtatta actgctttta tcaaacctat agttcctatt
    36361 gaaatcaaat cttctacatc tatacctgta ttttcaaatt ttctcgatat gtagactact
    36421 aatctaagat ttctttctat taaaattgac tttacacttt catctgtttc tagcttttgt
    36481 aaaagctcca tttcttcttc tggtttcaat ggaggaggaa gtatatttgc ccctcccata
    36541 tagtatattc cctttggctt tattattttt aatttaatac ctaacatagt aataaagctt
    36601 attattcttt ctttcaatcg taacattcct tgtccccctt aaattttcac ctaaaatcca
    36661 ggcaatatat ttggattcat tattgctcca tattctctat cattaaaatt tgaaatacca
    36721 agtattatat ttcctatctt ttgctcatct acttctatat agtcagcttt taagcctatt
    36781 attatgcttg tcttattact tccagcatgt ttataaggta ttattctaac tctacctgaa
    36841 gttttatcat ctaaacttcc tataatttct tgtgctttct tcatatctat atcttcataa
    36901 ttaatattta atagtttttc tggaataaat ccttctaaca cactagactt aactattatt
    36961 acatcacttt gacttattgg gtcttttaac aaattaccac tatctactaa agccttacag
    37021 caaaaacttt tttctagtaa gtttatattt atagtctttg taagctcctt aatgtactta
    37081 agcattttca aatctttata aatgtatttt aataattcac aactcacata agtacatgca
    37141 attaagaaag atatttttaa gtgacttatt cctgtaaaat aaattataaa ataagtgctt
    37201 ccagaaataa aaatgtttac tagataaaaa actaacgcta catgaacata atcttttttg
    37261 ctcttacttc taaatgaaat taaagttatt atactcatga ttattatttt aaatggtaca
    37321 gtaaatagta tattcattga tggatacaaa taagcaacag aataggcacc tcctaaacta
    37381 gcccccaata acttattttt tttaggatta tatcttttag taagaataga agtacaactt
    37441 ataataatat aatttattac taggttttct ataacatagt actcaatata caccatgtat
    37501 ccccccttaa aacctttaaa catattataa gatagtatgt acaaaatttt atgtcacttt
    37561 tgatggtcga atccaaaaca aattctattt aaaaagaaca atgatatatg tcttatactt
    37621 tatgtcgtca aaagatattt ttttaatgcc aaacaaatag acttttttgt agaatccaat
    37681 atatctaggt agaagttatt cgctctactt ttggtatatt tacaaaaaaa gactacataa
    37741 tttaataata tatcaagtaa atcttttact tatataaaat caaattatat agtctgtaaa
    37801 ttcaaatata aattattgtg ctagtaatcc atttgcatat tttgcagatt catttagtgc
    37861 ttgttctgga gttatgtctc cagctatcat catttgaagt tgtttccata aatattcata
    37921 tatctctact ccatgaggtc caactgtcaa tggtctatac atatgtttat ctcttgtcac
    37981 catttcttca aatcttgggt ctccttggta tttttcacct ttagcaactg gagctctagg
    38041 tatttctttg tggaaagctt ccattgattc tttgcttagc atatactcaa gaagtttaaa
    38101 tccaagttct ttatttttac atgctgacat taatgttaaa tcatctactg aagcaaaact
    38161 tccaactgtc ttatcttcta atgatgttac atatccccag tttaaatccg ggaatgattt
    38221 atcaaatgta cttgtagcag cagaggatag cattatagta aatgcagctt ttcctggtcc
    38281 aaatacactt tcaatcattt cattactatc ttttgataaa gcatcttctg gaatatactt
    38341 tttcatatca tttataaagt ttgcagcttt caagccttct gcattattaa atttaactga
    38401 ttttaaatca tcattatata tatctccacc agcttgccat aaatacggat accagttcca
    38461 gtttaagctt ccaaatactt tagaacccca accttgtgca agaccccact ggtcgatttt
    38521 tccatctcca tcagtatctt ttgttgcttt ttcacagatt cttttaaaat catcccatgt
    38581 ttttggaggt ttttctccta atttttctag tatatcttta ttgtagtaca atacgcctgg
    38641 attagcagct tctataggta aaccataaat accacccatc atttttgcat catctaagta
    38701 tagataatta tctgaagttc ctgatttttc taaataaggt gttaaatctt ctaccgcacc
    38761 catttctata aattgaggaa acatctctgc atacatgtat cctatatctg gaccttcacc
    38821 tgcacttatt gctgttgcat atttttctgc gtagttatcc catggtacta tctgaaattc
    38881 aatttcacag ttattttctt tttcaaattt ttcaaatatc ggcttccatg catctgcatc
    38941 atggtctgtt aatggtgcag accatacaac taatttttct ttaccctctt tgcctttaga
    39001 atcttctttt tttgaacatc ctgcaagaat tgatactgat agaatcccta ccataactaa
    39061 agaaagaacc ttttttagtt taatcatgct taatcccctt tcagacttta atttgtcttt
    39121 ttcctcaaat gtttacattt aattcagatt taatattaac aaataagatt actcttgtct
    39181 aatcataaaa aactatacta actcctataa ctatattttt tttctatacc tattagattt
    39241 aattcatact aaaaacttac tttttaaatt ttatcaatat ttaaaaattt ttttaagtct
    39301 agccacaaac taactatcta aattgagcaa aataaaaaga agtctattga cttctttata
    39361 agcttacata ttatcttctt cttcttagga atgtaggtat ctccatgctc tcatcatcat
    39421 cttcttcaat tatacttgcc tttttaggag gctctatttt ttcctcagca attacttctt
    39481 cttcaatttc ttttacagct tgttttactg ttgtatttaa tgaagaacgt atgcttgatt
    39541 taggtttagt atctaaatct aaggcaccat tttgtaatcc ttcaaatcct gtcgcgatta
    39601 ctgtaatcat tatttcatca cctaaatctt cttttataga agctccaaat ataacatttg
    39661 cttctgggtc acaggattcc ataactaaag ttgatgcctc attaacttca aataatccta
    39721 agttaggacc acctgtaaca ttaagaagta ctcctttagc tccttgtatt gatgtttcta
    39781 gaagtggaga ttgaatagct tgtcttgcag catcaattgc tctagtttca ccactagcac
    39841 tacctatacc catatgagca agtcccttgt ctttcattat agtagtaaca tctgcaaagt
    39901 ccaagtttat taacccttca actgctataa ggtctgaaat tgattgtata ccttgtttta
    39961 acacatcatc tgcaactgca aaagcatcta acattgatgt atttttttgt actatctgta
    40021 aaagtctatc atttggtatc gttataagag tatctacttt tgatttaagt tctgctattc
    40081 caccttcagc gttcttcatt cttatcttac cttcaaatgc aaaaggtttg gtaactacac
    40141 ctactgtaag tatccccatt tcttttgcca agcctgcaac cactggagca gctcctgtac
    40201 cagtaccacc gcccattccg gctgttacaa agaccatatc agctccttga agtaacttta
    40261 ctatttcatc tttactctcc tcagcagctc ttttccctac ttctggattt gctcctgctc
    40321 caagtcctct agttaatttt tctcctattt gaactttata ttcagctttt gatgtatata
    40381 aagcttgttt atctgtattt actgatataa attcaacgcc ttttagttga gcttctacca
    40441 ttctattgac agcattgttt ccaccgccac ctacacctat tactttaatt tgtgcacatt
    40501 cttctaattc tacgtcaaag tttagcatca taaaccccct tctctcatga ttcaaagtta
    40561 tcgtatgtat taattatatc attttcttct tttattttgt taacctttaa gtaacatatt
    40621 ccacaaaaac gtaaattttc ctttttaaat ttcttactta tattgtcaaa tattactctt
    40681 taagtataaa ataatttacg tatatattaa atgctcttta atttttacgt tttttgttca
    40741 taatattact aataatatat cgtctaatta tagataagtt attaaacaat ctcataccaa
    40801 aaactaatac agcaacatag taaagagaaa ctcctaactt ttctcctata taagtcaata
    40861 atatagccaa tatagcatta cctatgaaac cagtaataaa aattacatta tcatagttat
    40921 cttcaaaatt ggattttatc gctccaaaaa tagaatccat tattgcaaga atagctactg
    40981 acatatatat ggaataatcc atagaatatg taaatggaat atagtaacct actactacac
    41041 caaatgctaa tcctcctaaa acccataaca taaatcttca cctctttatt ttactttgaa
    41101 acacaactac attacaatcc tttttattaa ttttttttac tttttcttta tttttttgta
    41161 tttttcgttt tattttgcaa ataaattcac tttatttcca ttattttcct gaacttttat
    41221 attaattcca tatatatcct taagagattt agcatatgat tctggagagt ttatagctgc
    41281 actcaataaa tctatatctc ctatagcttt tattataaat ggttgtccat atgtagtatc
    41341 atttactgta atagtagctc ctgaacattt tatcttactt gttgctagta ctctttctcc
    41401 atttatagaa attgctcttg ctccagcctt ttttaaatca tttaaaaccc tcaaaatatc
    41461 tatatcatgt ataatcaaat cgtttggatt ctgtccgtct tttaactctc tctcactatc
    41521 cctcatagta atagttatgc ctggccctgt aacagttgaa taaccactta attctttaac
    41581 ttcttcaagc tctttttgca taagtgtttg gatagattca tttccattct tttttgcttc
    41641 tttatattca ttcaaagttt ttttgttctt tttaagagtt tcttttaagt tttttatttc
    41701 tttttttgta ttttctacct cactttcaat actttcagtt tgacctaatg ttatgtatac
    41761 tctatttggg tctagtgatt ttatataagt tgatatgaaa acgcctaata tgaatgctcc
    41821 taatataact attttctttt tcatactatt atttcccctc tgctttgttt atatcactat
    41881 gaccatttat ttctatattg tcttctttgg tcacttttac tttggcatta taatatgttt
    41941 ctatttccca gacaatacca tactttatat taagtgccga agccaatgta tcagaatcac
    42001 caattgcttt tattataatt ggttctttta tgacagtgtc attcatataa agcttgtctt
    42061 cttttaaata catatatgta ttagaaacta ttctctggtc gtttatagat atggcacttg
    42121 cttgtgctga atttaattta tttatcattg ataataataa atcataatta tatagtatac
    42181 tatcattttt atcagagttt cctaagtctt ttgtagaatt tatctcaatt tttatgcctg
    42241 ttccttcaac atctgtatat ccagctaatg tttcatattt ttctatttct gacttaagta
    42301 tgtcttgttc tccttcattg tttttgcctc tataaatctc tatatctttc ttcacactat
    42361 taacttctga ttgtagattc tctttttctt tttttaaaga tttaagttct tctgtaagtt
    42421 gctctcctct tttaaaagtt gtcatccctc tattttctat attaataatt ttaagttgta
    42481 aactaacaag aacacccaat acaatacttc ctatgattat acttttattt ataaatcttt
    42541 ttttcaatat atcacttcct tacaattgtc tatccttctg gactatacac tgccaacttt
    42601 ccatagttaa gatttatagt ccctccatta gtatttttat tttgcaggtc aatcagaatt
    42661 ttacttaccc tagatatatt ataatctaag ttatcatcat tagaaagtaa gatttttata
    42721 ttacttcttg taagcatttc tatattgctt tcttttttta agtttacata atttattttt
    42781 ctataaattc cttcttcttt taaataattt aaagttttaa atacattctc cttagtttta
    42841 taatttttaa attttataga tttattatca tcaatgctat aatctacatc tactatcaag
    42901 tcttttttac tttcattaga acctcttagt tcttccaata aatttccttt tttatctata
    42961 taacaataat tatcttcatc ttttaatact gcaaatattt ctttttcttt taaacttata
    43021 attatcttat tgggaagttt tctttttatt tccacatttt ctatatatgg attttttata
    43081 agtttatttt tcatatcttt aaaattgtat gcaaaaatat tttcatttag atttacattt
    43141 agttctttca ttatatttga tttggtcacc ctcttatttc caattacatc tattttcttt
    43201 acatcaaata aatcactttt tacaattata catatacaaa aagataacat aagtataaaa
    43261 actaaaacta tcatgacatt attcgtattt aactttcttc tttttttcaa atataatcaa
    43321 cccctttggt taataaatac acctttagag cttgtttttc ctacaaaaaa attataatat
    43381 ttaaagtcaa atttttgcat tttattacat aacatccaaa taaggtatta aattttataa
    43441 ataaatcaat atttcatcaa aataaacata gcatactaca tactatttat atatttaaat
    43501 ctgtatacat acaaataaac ccctaattta taataattta gaaaatctaa tatattataa
    43561 attaggggta aatatttact tatcttttct ttattcctat taccttagct tgaccttcta
    43621 tacttggagt cgtttctttt tttatttctt ttacttcctt aataactttt tctttttttg
    43681 tttttttaga agttgacttt tgtgttgaat tgtatacttt cattatctca tcatatataa
    43741 ggtctatggc ttcaggctta cctatagttt tacttgcatt tgccatatca accaaaagtt
    43801 ctctatctcc taaaagttta aatactgctg tatttaggct ctctggagtt agattttttt
    43861 ctaaaatagc aattcctgcc ccttgttttt ctatactctt ggcattatac tcttgatgat
    43921 tttcagcagt ataagcttta ggtattatta tagatggctt tccaagagcc gttatctcag
    43981 ctaatgaaat tgctcctgcg ctacctataa ccaaatcact tgctgcaagt gcatttgcca
    44041 tatcttctaa gtaaggcaca actttttggt atggtttaag atttatatcg ctaatacttc
    44101 ccataaattc atcataatag cttttgcctg tagcaaatat aaaagctata tcttcattta
    44161 ccatattctt tattacaagt ctcattgcat catttatttt tcttgaacct ccactaccac
    44221 catagcaaag taccattctt ttttcgtcac ttattgatag attctttctt gcaatatttt
    44281 ttcttgataa taaaatttct tttctaactg gatttcctgt aaaaactaat ttatcttctg
    44341 ctgcctctgg aaatctttta tgcgaatctt caaaacttgt aagtaccttt gtaactgttt
    44401 tagataaaat cttattagtt acaccaggaa aagaattttg ctcatgtatt atggctggta
    44461 ttttccccat agatgcatta aataaaacag gtccactaac atatccacca gttcctataa
    44521 ctatatctgg tttaaatttc ttaacaatct ttcttgattg ctccaaacct ttaaaaagct
    44581 taaaaactct ttttacatta tcgaaatcta tttttcgctt aaagccttga actgttacag
    44641 tctttaattc aaatccatac ttaggaacta tttctgactc tattcccttt tccgtcccaa
    44701 caaaaattat ctcagcgtct ggatgctcat ctcttatttt gtttgcaata gctatagctg
    44761 gatacacgtg ccctccagtt ccaccgcctg ataataaaac tttcatcaaa tcatcatctc
    44821 ctgttaatta attttgacat gctttgagat gtttaacact attcctacag ctcccataaa
    44881 gatggtgagt gaagttcctc cataacttat aaatggtagt gctacacctg tagctggcat
    44941 tgatgatgtc gcaactgcta tatttaaagc tgcttgtatt ccaatttgtg cacctatacc
    45001 tataacaacc atacaagcaa atacattaga agtcttcaaa gctattctta cacatctgta
    45061 gactaaaact acaaatagca taataactat tatacaacca atcaatccta actcttctcc
    45121 tattatagca aatataaagt cattttgtgg ctctggtata taaaaatact tttgtttact
    45181 ctttcctaag cctagaccaa acaatccacc tgaacctagt gcatatagac cttgtattac
    45241 ctgatacccc ttaccaagag ggtcttgaaa agggtctaaa aatgatgtaa cacgactaag
    45301 tctatagggt tcagcaatta ctaatgcagc aaataaagca gccccactcc ctatcatagc
    45361 aaatacaatt ttcatatcca ttccagcaac aaatatcatt acaaaagtaa ctagaattac
    45421 agtacctgct gttgacaaat ttggctgaag tattatcaac gcaaaaaata tccctggaac
    45481 tacgagaagt ggtacaacac cttttgtcaa agattttatt ttatcataat ttttttcaat
    45541 taattttgca gttaatatta ttgtagcaaa ctttgcgatt tctgcaggtt gaaatgttaa
    45601 agctcctatt cccaaccatc tttttgctcc gtttgcttct atccctaaag gggtcaaaac
    45661 taacaataat aatactactg ctatagctcc aattgctgta gcatttttct tccaaaaact
    45721 ataatctatt cttgatgtaa taatcataac tataaatcca agtacagcat atattacatt
    45781 cttttttaga aaatagtacg catcattgtg tttaaatgaa gattgtataa aacttgcact
    45841 aaacaccata actattccta caaatactaa aagcatagta gtgtaaaaca ctactccatc
    45901 aaattcagtc ttcatcctta tgtcaatttg ttttttcaaa ttttctttag gcatccaact
    45961 aatctacctc cattcttcca tttaagtaag tttagaggta agagattatt ttaaattgtt
    46021 tacattatct ttgaagtctt ttcctcttac ttcaaaactt tcatacatat cccaacttgc
    46081 acaagctggt gaaagaagca ctatatctcc actttttgaa atttcataag aagtcttaac
    46141 tgcttcttcc atatctttta ctatatgtat gtcattaaat cctttatttt tagcacaatt
    46201 ttctatattt gatgcagttt ctcctaataa gaccaatgat ttaacatatg actttgcagt
    46261 ttcaagaagt tcatcaaaat tacttccctt atccatcccc cctgctataa gtattatagg
    46321 tctatcataa gactgtatag cttttatagt ggaatctgga ttagttccct ttgaatcatt
    46381 tacatatatt acattgtcta aatttcttac aaactcttgt ctgtgttcca ctcctttaaa
    46441 tgtcataagt acatccttta taacttctaa atcaattttg cagacatatg ctattgctat
    46501 agctgccata caattttcta agttatgacc tccaggtaaa cttacatcat ctttatttag
    46561 aaatttgatt ttatcatcta tatctattat tatgtcatta ttttctaaat atactcctcc
    46621 atttactttt ttagttcttg aaaagtaaat tactttggca ttgcatttat cagctaaaga
    46681 ttttactatc tcatcatcat agtttaatac acagaaatcc tcaacagttt ggttcataaa
    46741 tatatttgcc tttgcttcta tgtatttttc catagtatgg tgtctattta aatggtcctc
    46801 tgtaatattt aatattgcgc ttactttagg tctaaattca tctatactct caagctgaaa
    46861 actgcttagt tcagttacta acacagactc ttcacttgaa gtttctatag tatctattac
    46921 aggatttcct atattaccaa ctacatatgt atctcgtttt gctcttgaaa aaatttctcc
    46981 taccaaacta gttgtagttg tctttccatt tgtacctgta atccctataa aaattggatt
    47041 attcgatagt ttaaaagcta attctacctc accaattata tacttgcctg aattttttaa
    47101 tttcaatatg aatggtaggt ctagtggtac acctggagac actacaacca tatctatatc
    47161 atctacatct tctgggtgat gtcctaatat gtattttatg tcatttatag atttcaattc
    47221 atcaagtata tttctcaatt tattttcatc ttttatatca tttacgatta cacttgcacc
    47281 caatttatct aaatgtttaa ttgtagatat accagttttt gcaagtccta ctaataaaac
    47341 tttttttcct tttaagtcca tttttcattc cccctagccc taaaatactg ctattatact
    47401 tatccaagct aaaacaactg ttataatcca aaaaataaat acaactcttg tttctggcca
    47461 accacactgt tcaaagtggt ggtgtattgg agccatctta aagaatctct ttcttgttgc
    47521 cttaaaatat ccaacttgaa tcaaaactga tagagcctcc gcaaaataaa ttcctccaat
    47581 tataggtatt ataagcactg agttagttag aactgaaaat gctacaactg cacctcctaa
    47641 tgccattgaa cctgtgtcgc ccataaacac tctagccgga tatgaattaa atcctaagaa
    47701 acctaaacat gctccaacag ttgctgctgc taaaactgcg acttctgtat ttccagcaat
    47761 agaacttgca aatagcataa agaatacaga aactattaat gttactcctg atgctaatcc
    47821 atctaatcca tcagtcaagt ttacagcatt tactattgct acgattataa acatcattat
    47881 tggtatatat aatattccaa catttatcac aaaatctgta aatggtatca taagttgtga
    47941 agcacttgat gatgatgtat actgataaaa agcaacataa aaagataatg caacttgaag
    48001 gattattttt tgatatgctt ttagtccaag tgatcttttt agctttattt ttataaaatc
    48061 atctaaaaag ccaataaaac caaaacctgc aatacatata agacctactg ccatatcatg
    48121 actaactttt actcttgtaa gtccagttat tagaattgca acaatcatta taattccacc
    48181 catagtaggt gttccgtttt tagctagatg tgtttggggt ccatcatctc taactgtctg
    48241 accaaattta aattttctaa gcattggtat aaatattggt cctattataa ttactattaa
    48301 aaacgcaatc aatgcggtgt acgtaagctc tgttattcct aacatcataa aactcctctc
    48361 ccttgtcaat taactcatta gtttaagctt atttatttag atattcgact attttttcta
    48421 atctcatgcc tcttgagcct tttactaaaa tagtgtctcc tgtttttact aattcattta
    48481 acttgttaaa cacatcttct ctattctcaa agtgatatat attggctgaa ttgaatccta
    48541 attgttttgc ttcttctcca ataaacactg aattttcacc tatagttatt ataatatcag
    48601 tattattcat agatgatttt ccaactaatc tatgaccata ctctgaaatt tcacccattt
    48661 ctagtatatc tccaagtatc gctacccttc tacctttata tcttccaagt atctttaaag
    48721 cagcatccat agaatctgga ctagcattat atgaatcatt aattattgtt aatttttcat
    48781 tttttattat gtctaatctc atttttgtcc ctttaaaatt ttttaaacct ttttttatat
    48841 aatctaaaga aatatttaga cacaaaccaa ctaatatagc agacattgca ttatatatat
    48901 tatgttctcc tattgttggt ataaatattt cctctttttt cccatttatt acacatataa
    48961 atgttaaact atcctcttcc atagtataac tttcacaata aacatcattg tttttattaa
    49021 agccaaatgt ttttaactta tatacatgtt ctttttcttt taatgtagat aaaaacttat
    49081 catctccatt tacaattaaa gtatttgttt catcaaagtt tgaagtaatc tccatctttg
    49141 ctttcaaaat accctcttgt gaacctagtt tttctacatg tgataatcct atattcgata
    49201 tgactgcaat tttaggattt actatatcca ctaaatattt tatttcattg aatccagaca
    49261 tacccatctc tattacagca cactcatgtt ctttatttag attaaataaa gtcaatggaa
    49321 ctccaaaatg attatttaag tttttttcat tttttagtat atttaacttt gcagatatgg
    49381 cagagtaaat catatctcta gtagtagttt ttcccacact acctgtaaca cctataaatg
    49441 gaatatcaaa tttttcttta tagtattttg aaatatctcc tagagccaaa gatgtatctt
    49501 ttacctctat taaatttata tccgaacttt ctaattttat tccattactt tcatttttta
    49561 taaaagtttt acatccacta tcatatgcag acttcataaa tgtatgccca tctaaatttt
    49621 ctccaactat ggctacaaat gcattttggg catttgcctc cctgctatct ataactatat
    49681 cacttacaca gtcattataa tctccatata ttaacttccc attagttgca agtactaact
    49741 cttttattgt taagcactcc attattaaac tcctccttta aaaccttagc tatattctct
    49801 tagcaacaaa aggcaatgta taccgttata tatacattgc ctacgataac tcttaacttt
    49861 ataattatat actatatttc tgtttttttg aagatgttta tgggtatatg ttaattatct
    49921 gttacttttt agttttcaaa atatagggtt atacttgagt cttcattaac ttttactcca
    49981 ggttttggga atatatcctt tacctttgta tcttctggaa gttctatatc tgcatctaag
    50041 tctggtttta acttcgcatc ctccaatgcc tttacagcat ctcctatttt tagatttctt
    50101 acatctggca ctttaacttg cttcttttca tactcagctt tttcttcttc tttatatact
    50161 ggtttaactc ccaaatattt taaagagtca ttcattattt ctttcactat tggtcctgct
    50221 gtagtactac caaatgcact tactcctgtt ggttcgtcaa caatagcaag aactactatt
    50281 tgcgggtcat cacaaggtgc tattccaaca aatgaacata tatattttcc tggagcatac
    50341 ttaccatcta taactttttg agctgtacct gttttaccac caagtctata acctggtata
    50401 tatgctatct ttcctccacc ttcagttaca actgattctg caatttctaa cattttttta
    50461 gatgtttctt tagatataac acttcttact ttttttggtt ttactgtttc agttatattt
    50521 cctttattgt cagtataaga ttttaccact cttggttgca ttaaatctcc accattagct
    50581 attgatgata tagctgttat aagttgtatt ggagttactg atatagattg accaaatgat
    50641 attgtagcca attcaactgg tcctacattt ttttcgttat acagtattcc cttagcttct
    50701 cctggtaaat ctatacctgt cttatccata agaccaaatg attctatata atcatacatt
    50761 tttccaacac ctaatctgct tcccaactca acaaatactg ggttgcaaga gttttgtact
    50821 gcttgtttaa attcttgtgt tccatgaggt ctgtaatgtc tccaacattt aatctttctt
    50881 cctccaacag ttacactacc tgtacatgtg aatttttctc catctttaat tactccttct
    50941 tcaagagcac tggaagatgt aataagctta aatgtagaac ctggttcata tgtatcactt
    51001 actgctggat ttctccacat ttgataatag ccttttattt tatctttatc attatatttt
    51061 tcaagttctt cttgataata tggatatatt ggcgttcttg agtcattagg gtcataatct
    51121 ggttttgatg ctaaagcaag tatatcacct gttttagggt tcatagctat tgcagtaacc
    51181 ttttttgcat tatttaactc ataagctttc tgtactgctt tttcagtata gtgttgaatt
    51241 acttcatcta tagacaacac aagaccatta ccttgtacag gctcataata tttttccatt
    51301 ccttgtggta tttctcttcc tgaagcatca gtacttacta taagttttcc aggctttcct
    51361 tttaactttt tatcatattg catttctaca ccagatatac ctgttgcatc tgaagaagta
    51421 tgtcctaaca catatggtgc aaaattccca tatgggtaat atctttgatt atcttctgcc
    51481 acccatattc cacttaattt agcatctctt atttgacttg ccttatcatc atctatccat
    51541 ctttttactt ttacaagagc catatttttt ttgcttatta aagcgtaaat atctttataa
    51601 tcttcatcca aaatctcagc taccttttcg gctgcttctt ttttatcttc tacttccaca
    51661 ggtttacacc aaacagtata cttagttaca cttactgcta actctttcat atttctatca
    51721 tatatagttc ctcttttggg ttctattggt atatctcttg tttgttgttc tagtgctttt
    51781 gtactcaacc aatttccctt cataagttgt agatacccag tcctaataac aaggcaaaaa
    51841 aacaatgcac atgctagaat aagaacgagt accagccttt tcttacttat cctctttact
    51901 tttctcaaat ctattccccc tagttatcca catctatata ctttatttgt ccatcttttg
    51961 gatagtccat attgagcaat tccttagcct ttagctcaat ttcttttatt gatgtttttt
    52021 cagtttcttt tgcctttagt tcctctaata ctatctcttt ttttcttaaa tctttttgta
    52081 aataatagat atcgtacttc atttccgaga tttttgaata accacataga ttcaatatta
    52141 ttactgaaaa agttgcaatc agtaaaatgc atttagctcc tatttttttc ttatttctct
    52201 ttttataatt attctttttt ttatttttat attcatttag attatgtatt ttataatttt
    52261 ttttcaaatt ctcaccccga tagtatatag tttaaatttt cctctatact atatttgtta
    52321 ttaatcatat ttcttcctct aaaatttcat cgctaaatta atacactatt acacaatcta
    52381 attgtatttt tttctataca caacaaaaac ctataaattc acttatactt ttatatgaat
    52441 ctataggtta gatattaatt tctatttatt tttatcactt ctagtatttt tagataattt
    52501 cttccataaa tataatatgt aactaactat ctactctctg attcctaagt tctccattat
    52561 attacttaat accatctata tctatatttt tatatctaaa ttttcattat atatctcaat
    52621 aacaaagaac atttcttata gtgttaattg ttttttaatt gctttaccag tgcaacaaaa
    52681 tatataatac tttttaatta attataataa tcttatttta tatataagat tattctttag
    52741 ccttataact acttatattt tttctgctac tcttagctta gcacttcttg aacgtggatt
    52801 tacttctatt tcttcctcac taggtaatat aggttttcta gttattattt tcacctcaga
    52861 ttctttatca cattggcata taggtaaatg ttgtggacat atacaatctg atgctaaatg
    52921 cttaaatgca tttttcacta ttctatcttc taatgaatgg aatgttatta tacatattct
    52981 tccaccttca ttcattatag aagaagcatc atttatcatc tttgtaatta ctccaagttc
    53041 attatttact tctattcgta ttgcctgaaa agttctttta gctgggtgag gtccatctat
    53101 tctagctttc ttagggatag cttttttaat tacatctact aattctcctg tagtttctat
    53161 tggtttgttt gctctttctt caactataaa tttagctata cgcttagccc agttatcttc
    53221 tccataatct tttattatct tagttaattc atcttctgta taagtattta caacatcata
    53281 tgcagaaaac tcacacctaa catccattct catatcaagt ggtgcatctt gcatataaga
    53341 aaatcctcta tcagcttcat caagttggtg agatgaaact cctaaatcag ctaatacacc
    53401 atcaattttg taaactccta ttttttctaa ttcctctttt atattttcaa aattgctgtg
    53461 aacaaacttc actctacttt catattcact caatctctct tttgctgtgc ttatagcatt
    53521 tttatcttgg tcaaacccta taaataatcc cttatctgac agttttttta ctatttcttt
    53581 agaatgtcct gctcctccca tagtacagtc aacatacacg ccatctggct ttatatttaa
    53641 attctctata cactcattta aaagaactga tacatgatga aattccatag ttacctccct
    53701 taaattcact ttttaaacta ttcttcacta atattatttt tatttttctt tgataaataa
    53761 acatgtgcaa ttatgccacc aataactcca attagactta taacttgtgc cattctaagt
    53821 ggtcctaaca ttaaactatc tgttcttaat ccctctataa aaaatcttcc tattgaatat
    53881 aaagttatat aagtaactat aacttgacct tcatattttt tattttttct aaatagtaat
    53941 aaaactataa atattccaaa atcccatata gactcatata aaaaagttgg atgtaccttt
    54001 actccatcaa ccattattcc ccaaggtaaa ttggtagcac ctccatgtgc ttctccatta
    54061 ataaaatttc cccatcttcc aatagcttgt gctaaaggca ttccaagtat aactgtatca
    54121 gccattttta agaagttaat atttttaatc ttagtgtata tataaccagc taatattcca
    54181 ccaattaacc ctccatgtat agcaagtcca ccacctctaa aattaaaaat ttgagacata
    54241 ttttgagcat agtaactcca attaaataca acataataga ttcttgcaca gattagtcca
    54301 actggaattg ctattatagc tatatttaat acatcatctt ctttaattcc cactcttttt
    54361 gcttctttta ttgctattaa agttcctaaa atcattccac atgccataag tattccatac
    54421 cacatgatgt ctatgccaaa cagtgtaaat gctactctat ccataacgtc acctctttgt
    54481 attaattgaa aatatttcca aagcaatatg ctataattac aattaattgt aattatacta
    54541 aattatgata acacattaat gttaattcta caaaaaatat taaaatttat tctacaactc
    54601 cctacactat attatatcta attactataa aattttttaa actgaatctc tcatgactaa
    54661 agttacaagt gtacatagat attttttaat caaaaagatt gatgcatata tacatcaacc
    54721 tcttctaatt cttatctata gctcttaaat ttttacctca atttatataa atatatttta
    54781 taaatttgtt ttaccaaaat aaacatttaa aaacataaac taatacttaa ttagttattc
    54841 ttattatgat taagtttcta tttaggctct acaatagaaa taacacaata ctcttcttta
    54901 aaatgctctt ttaatctttc ttcaacttcc tcaaaagtaa tatctttaat aacatctaaa
    54961 tactccaata gatttatatc cttaaaaaca taagatataa aactattacc tataaagttt
    55021 atagaatcaa agcacttaat aaatgaccct atttttttct ttttagttct ctcaaactct
    55081 tcttttgata atccttcttt ttttgatttt tctatataat caagaattat ttcttttact
    55141 tttttaggct ctttagaatc tccagctatt atgctaaaag cataatccac ttgagacgaa
    55201 aatccagctc caaaattttc atttattaaa ccttgcatat acaaatcttc ataaagctta
    55261 cttccttttt taaataacat ccctacaagt atatcagtta caatctcttt acgtaaaagt
    55321 tctttccctt ttaaaccaac attactatct ttaaaaccta tgttgaacat aggcatagat
    55381 atagggaatt tttctataac ctctttttct tttacacctt ctggttcttc tggataaaat
    55441 ctttctatac tcttagagag tttatctact ttgtaattgt ttgacttttt agttacatct
    55501 ataacttttt ctacatctaa gtcaccaaca acaaataaag ccatatttcc tggattatag
    55561 aaagtattat aacatttata tagctcttcc ttagttatct tatatatact atcaacagta
    55621 cctgctatat caattctagc aggataattt acatacattg ccttcaagca attaaagtat
    55681 acattccaat cagggtcatc attgtacatt tttatctctt gtgctattat acctttttcc
    55741 ttttcaacat tttcatctgt aaaataaggt gtttgaacat agtcaattag atgttctaaa
    55801 ctctcataaa aattttcagt agcagaaaat aagtaggctg tcattgtaaa attagtaaat
    55861 gcatttgcat ttactcctaa ttttgaaaat ttatcaaatg catctcctcc atcaggttgc
    55921 tcaaacatct tatgctctag aaaatgtgca ataccttcat tcactctaat tttcttatct
    55981 tctccaattg gtacaaattc taagtcattt gaaccataat tagtagctaa aatagcatac
    56041 tttttcataa aacccctttt aggcataaaa tatacatcta atccattttg caatttctca
    56101 taataaactt cttctttcaa tatatcatta actatttttt ccatagacat ctcctccatt
    56161 aatttctcaa gaagtataca gtatcaagct ctatattttt tatagcttca actatatctt
    56221 tttttgtaac cttttctata ctagatatta tatcttgtac ttcagaattt gtctttgcca
    56281 tagattgaga gaaaacaaaa tcagacatac cacctatatt atcagttata gatttcatag
    56341 aattaactaa agcaagttta ctgttttcta attcttcatc agatatatca cctgctttta
    56401 cactttctac ttgttttttt atcaattcta cagctttatc ataatcttta gtttctatac
    56461 cagatgatat aaacatgata cttttatact tctctattga agagaatata tagtaacata
    56521 aactttcttt ttctcttaca ttaacaaata atttagagtg tggaccacca cctaagacat
    56581 tgctcccaac tactaaagca taatatttat ctatgtctgc ataatccaca tttgctctat
    56641 aacccataac taacttacct tgagttattt ccatttgttc atctataact tttacctcat
    56701 ccacattttt tataaaatct gctcttggta tatttataat ttcttctctt tcaaatttta
    56761 gatttttact tattatatct acaactttat cttcatcaaa attaccttct acaactatat
    56821 ctataggtga tgttttaagt atgtttttat aatgttcata taactctctt gaagttatac
    56881 tatctatttc atcttcataa ccaaattcac ttacactata tctctctcct ttacacatag
    56941 cctcaataca tttatcttgc gcatattcct ttttatcatt tattattgat tgtatctttt
    57001 ctcttaaatt ctctttttca atatttaaat attcttcttt aaacccacca tctacaacaa
    57061 gcgtattatt tattacttca ttaaaaaatt ctactacttc ttcaaatata ctctcatcta
    57121 aatacttttc acttatgtta attactttaa aactcaatac ttgacgttct ccacgtttta
    57181 cagcatcagc tcccatagat gaaccgtata aatcatctaa tttattggat attgctctag
    57241 cactaggata tttagagctt ccacttgcta ttattgatgg tattagagca ttttttgtag
    57301 tttctttttc atccaatagt ctctgaacat ataaacttat aagattcgat ttaaactttt
    57361 ctgtttttat caaagtaaga ttaatattat tacctaaatt tattattttt ttagtattaa
    57421 ccatatgtac ctcctatttt tatttaaaca tcaattttaa aattgaaatt tatttatttt
    57481 ttaattacat atactcacat ataaaaacct tctatttaat tatatccttt ttattatttt
    57541 ctaaaagcaa tatttttatt taaattaaat acaaatatta tttaaatcaa tatgctttaa
    57601 agtctagttt acaagcttga tatatatgat gtataatcaa tatgtatgtc tataattaaa
    57661 acaattttgc aatcctatag attgattgtt taaagttata taaagaatat aaaaaagaat
    57721 aagagagagg acgattaata tgaaattagg tatagttggt ttaccaaatg taggtaaaag
    57781 cacactattt aacgcaataa ctcaagcagg tgcagaatct gcaaattacc ctttttgtac
    57841 aatagaccca aatgtgggag ttgtatctgt tccagatgaa agattaaaca aactacaaga
    57901 attatataat tctgaaaaaa tagttcctac tgctatagaa ttttgtgata tagcaggttt
    57961 agttagagga gcttctaaag gagaaggttt aggaaataaa ttcttatcac atataagaga
    58021 agttgatgca atagttcatg ttgttagatg ttttgaggat gaaaatgttg tacatgtaga
    58081 tggttctgta gacccacttc gtgatataga aactataaac ttagaattaa ttttctcaga
    58141 tatagagatt cttgaaagaa gaattaataa aactcaaaaa gctgctaagg ctgataaaac
    58201 tttaggttct gaacttgatt tattaaaatc aataatgtct actttagaag aaagtaagtg
    58261 tgtaagaact atggaattta cagaagatga acaaacattc gtaaattcat tggatttact
    58321 tacatctaag cctgtaatat atgcttcaaa tgtatcagaa gatgatttag ctgataatgg
    58381 agaaaataat aaatatgttc aacaagttaa agcatttgca gaaacagaag atgccgaagt
    58441 tgttgtagta tgtgcacaaa tagaagcaga aatttctgaa cttgattctg ctgaagaaaa
    58501 gaaagagttt ttagaaacat taggtcttga acaatcaggg cttgataagc ttataaaatc
    58561 ttcttatgct ttacttggtt taatttcatt cttaactgct ggtccaaaag aagttagagc
    58621 atggactata aaagttggaa gtaaagctcc tcaggcagga ggaaaaatac attctgatat
    58681 agaaagagga tttatcagag cagaaacaat agcctttaat gatttggttg agcatggtac
    58741 tatggctgct gctaaagaaa aagggttggt taaattagaa ggtaaagaat acatagttaa
    58801 agatggagat gtaatattat ttagatttaa tgtttaaaat gattaatcgt atacactttg
    58861 actattatat ataataa
//
LOCUS       DAMJVI010000009        58820 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.009, whole genome shotgun sequence.
ACCESSION   DAMJVI010000009 DAMJVI010000000
VERSION     DAMJVI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 58820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58820
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.009"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            82..1176
                     /locus_tag="PC545_000674"
     CDS             82..1176
                     /locus_tag="PC545_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2455464.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTLMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTIALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            1327..1683
                     /locus_tag="PC545_000675"
     CDS             1327..1683
                     /locus_tag="PC545_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455465.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(1776..3044)
                     /gene="eam"
                     /locus_tag="PC545_000676"
     CDS             complement(1776..3044)
                     /gene="eam"
                     /locus_tag="PC545_000676"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HDF2455466.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEYIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTHIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPVYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(3446..4828)
                     /locus_tag="PC545_000677"
     CDS             complement(3446..4828)
                     /locus_tag="PC545_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2455467.1"
                     /translation="MPINSFENYPMTWKPKLDNRKPPIYKTLAMLLEEDIKRGNLNPG
                     DKLPPQRELADFLDLNLSTITRAFKLCEEKGLICAKVGKGTFISSDVNVSNTLLYQTE
                     SKDIIELGTVHPPYEQNTYIIDFIKNVLKQPEMDRFLQYMSPSGTYMQKKSIAKWIER
                     NNVYTSEENILLSTGGQNAICATLLGLFKAGDRIATDSLSFSGIKSIAKMIGIQLVPI
                     PQENNEISIEYLESYCKNENIKGIYLIPDYHNPTTHTMSDLSRKKIAKIAKQYNIIII
                     EDAINSIFRDGIQTPIFSLASDNTIYIFSTSKFLCAGLRVAFVVAPKRYIENLENALY
                     NMNLMVSPFTAEIVHRLLYSPIIDKIIEEKRDAIIERNEVADKVLSDYNLIGDKNCSF
                     RWLLLPDELDGKSFEISAKNLGVQVYCAERFSVGNSTVPKAVRICVTAPKDVEELEKG
                     LNIIKSLLKS"
     gene            4953..5639
                     /locus_tag="PC545_000678"
     CDS             4953..5639
                     /locus_tag="PC545_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDF2455468.1"
                     /translation="MKTIGLIGGMSWESTITYYQVINTVIKERLGGLHSSKCILYSVD
                     FQEIEECQSSGNWEKSAKILADAAIKLQEAGADFIVICTNTMHKVSDKIQESIHIPLL
                     HIADVTATVLREKEIKKVALLGTKYTMEQDFYKNVIINNGIEVLIPNEEDRIIVNDTI
                     FNELCLGIISESSKKAFLSIIDKLGKQGAEAVILGCTEIGLLIKQNDTSIPLFDTTVI
                     HAIEAALSSI"
     gene            complement(5824..7359)
                     /locus_tag="PC545_000679"
     CDS             complement(5824..7359)
                     /locus_tag="PC545_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455469.1"
                     /translation="MIFVKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKNPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNKVSAVGVGKATIYAKCGNKEIERTFRIQARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPVERAISLSSNGNVVGL
                     REGKDRVRITCGDKSKVITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNIDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMRVENISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNSSQNSDVI
                     NVR"
     gene            7634..8683
                     /locus_tag="PC545_000680"
     CDS             7634..8683
                     /locus_tag="PC545_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2455470.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEVGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MVGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSAGKGGISMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            complement(8732..9766)
                     /locus_tag="PC545_000681"
     CDS             complement(8732..9766)
                     /locus_tag="PC545_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455471.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKETNSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLKESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARSKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(9784..10131)
                     /locus_tag="PC545_000682"
     CDS             complement(9784..10131)
                     /locus_tag="PC545_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2455472.1"
                     /translation="MIRKKSELQVEVKENLNGGIGKVKLENIIQNEELKGKGRLFKRV
                     ALPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            10389..11771
                     /locus_tag="PC545_000683"
     CDS             10389..11771
                     /locus_tag="PC545_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2455473.1"
                     /translation="MSVLKTQEKLKQPRDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLAWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGLSISMYGYRYVFLFSSITVLIGLFFFIFGNRKFYKPLN
                     NDPSNGRESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVIQTGTDPNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISFLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSN"
     gene            complement(11852..13645)
                     /locus_tag="PC545_000684"
     CDS             complement(11852..13645)
                     /locus_tag="PC545_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2455474.1"
                     /translation="MNIKDRLSGLRKFMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDSIWPDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYNDVYEFVKNIDKTEKVLLDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEVELENIRNSHVKDGVAFTKFMYWLKKNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKAKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
     gene            complement(13898..15289)
                     /locus_tag="PC545_000685"
     CDS             complement(13898..15289)
                     /locus_tag="PC545_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2455475.1"
                     /translation="MSSTESVSKKSGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSIAMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEK"
     gene            15698..16162
                     /locus_tag="PC545_000686"
     CDS             15698..16162
                     /locus_tag="PC545_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDF2455476.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGSQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            16169..16663
                     /locus_tag="PC545_000687"
     CDS             16169..16663
                     /locus_tag="PC545_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HDF2455477.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            16679..17704
                     /locus_tag="PC545_000688"
     CDS             16679..17704
                     /locus_tag="PC545_000688"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HDF2455478.1"
                     /translation="MKENIVMKSRVRLARNLNNYPFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAENDKSAVIVKKDKTISIMINEE
                     DHIRIQTICDDLNLEYAYSVANEIDDLLESSLEYAFNTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRVMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            17691..20138
                     /locus_tag="PC545_000689"
     CDS             17691..20138
                     /locus_tag="PC545_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDF2455479.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKSLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWNKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            20318..21691
                     /gene="radA"
                     /locus_tag="PC545_000690"
     CDS             20318..21691
                     /gene="radA"
                     /locus_tag="PC545_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDF2455480.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVSINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     ESVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVVKETKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            21694..22764
                     /gene="disA"
                     /locus_tag="PC545_000691"
     CDS             21694..22764
                     /gene="disA"
                     /locus_tag="PC545_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HDF2455481.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAVTNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            22853..23947
                     /locus_tag="PC545_000692"
     CDS             22853..23947
                     /locus_tag="PC545_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDF2455482.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYLIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKEVKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            24076..26253
                     /locus_tag="PC545_000693"
     CDS             24076..26253
                     /locus_tag="PC545_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455483.1"
                     /translation="MRKKIISFLATFIIILTSASQYSFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSINENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KETYKNNDVIFVELGDTYRLDLYKPNLNEKTYESMKDNIEKNIDIYLKNIFSMVEEND
                     TIYIASAFPSDLDYKNKRRLSPIIKLNGEGKGILSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKNYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWYIIVFSLVILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVVFVLGVFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILVLMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYVIVPMITLSINLTALKE"
     gene            complement(26439..27284)
                     /locus_tag="PC545_000694"
     CDS             complement(26439..27284)
                     /locus_tag="PC545_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2455484.1"
                     /translation="MNRNNFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCIYQNTSILLQVPLSFLNKYIPDFK
                     NCYFDFKVNANDNNYKKNSSKVKLILENMREIKLSSPPAANLLFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTKINYKMPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILEAHGFTNTKLFYRIFKEKFKCTPKHI
                     RKKST"
     gene            27501..28667
                     /locus_tag="PC545_000695"
     CDS             27501..28667
                     /locus_tag="PC545_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HDF2455485.1"
                     /translation="MYIKGVNLGGWLVLEKWMTSSLFEGTEAEDEYYLPRQLSREAYE
                     SRIKTHRSEYITERDFATIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFAWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGICSWSQNPEYVAFTLNVLERLAKRYGMRHELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKEMARGSKPNSLEFLRDFYIKAYRVMRPYMR
                     EENVIVFHDAFELKAWKDFMREEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIREN
                     YAKDILQMQKYFPVICGEWSLFNSYVCGIDTNGGQSPLNGIESNIDKLSKDDKRELYR
                     KIAKAQLDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            28689..30335
                     /locus_tag="PC545_000696"
     CDS             28689..30335
                     /locus_tag="PC545_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDF2455486.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVYGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDDGKKWIASLDWETREGYEKPGVICL
                     VEYCTKKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHSVTMGR
                     AKNIWGPYEKDSMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETTSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTKDNWLRMEDESNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNELGLQYYAPRISPLSFADVKSRPGYVRIRGQESRTSLNKVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIQGRKSYFEWSYDEKNYQRIGKVFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIVDESKDVD"
     gene            30432..31805
                     /locus_tag="PC545_000697"
     CDS             30432..31805
                     /locus_tag="PC545_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2455487.1"
                     /translation="MNTEVNVKATTIRYEKISFFERISYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLVSRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     IAAVLLFSVPSNLGITSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQNQYERGVL
                     SIFRMILATCGTLLINGLTLPLVEYFGNNLSAWTKTFFVFGIASIMVFFITFTGTKER
                     VKAVKQDRNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFGPINMVSNISQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIVGQNHIG
                     VIFASAIKGIGSAGIASTMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGAVLSIGGYVGTSATQSDLAIVSIKVCFIYLPIFITILQIIIMSFYKLDKEYSTI
                     LNELNTK"
     gene            32250..33203
                     /locus_tag="PC545_000698"
     CDS             32250..33203
                     /locus_tag="PC545_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HDF2455488.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDALTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            33241..34203
                     /locus_tag="PC545_000699"
     CDS             33241..34203
                     /locus_tag="PC545_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HDF2455489.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSCFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLKNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            34228..35214
                     /locus_tag="PC545_000700"
     CDS             34228..35214
                     /locus_tag="PC545_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDF2455490.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAADAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGFVPEDDYVIEIGKADIKREGTDVTVITYGRMLQSV
                     EEAAETLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            35227..36273
                     /locus_tag="PC545_000701"
     CDS             35227..36273
                     /locus_tag="PC545_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HDF2455491.1"
                     /translation="MVANKIKATPAARSQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTGKETIVSTNTSKSSE
                     KKELKIENENSRMFNTVEGIFEKPNPMRATVAKRMSESYFSAPVFTFNIEVDATELKV
                     LRAKLIDTVKESTGVKLTMTDLIVMAVSKILPNHQALNSAWTDEGIFRYKDVNIAIAV
                     GLDEGLYVPVVKNANKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            36440..38170
                     /gene="lpdA"
                     /locus_tag="PC545_000702"
     CDS             36440..38170
                     /gene="lpdA"
                     /locus_tag="PC545_000702"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDF2455492.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAVIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEFKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVDVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPRMDKELSE
                     SLKYSLSKKGINVLTKKKVSEFKEEGNNILVCIEGEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGVNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPTDEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            38588..41536
                     /locus_tag="PC545_000703"
     CDS             38588..41536
                     /locus_tag="PC545_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2455493.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSRLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKEIQDSGDL
                     KLDKESDDKKEEIINNRENKDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFAVESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADENKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETRLTHKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKLKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIIGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LSSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPVEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRDMSEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEATTIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGINIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKCNNTKVDFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKAKLNQINNLIDTNQIGETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKKPIVWSKSKVKIILLLAVDQKCFNPLSTLLSFIETDEFKKIKNIKDRDFIRE
                     MILDGVNENN"
     gene            41514..41978
                     /locus_tag="PC545_000704"
     CDS             41514..41978
                     /locus_tag="PC545_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2455494.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKESYIQ
                     AVVAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            42091..42393
                     /locus_tag="PC545_000705"
     CDS             42091..42393
                     /locus_tag="PC545_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455495.1"
                     /translation="MGVTNVAKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLINDVLEELEL"
     gene            42455..43819
                     /locus_tag="PC545_000706"
     CDS             42455..43819
                     /locus_tag="PC545_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425524.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2455496.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIVMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFASVANMVIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGIAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSEAAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     SIEE"
     gene            43854..44327
                     /locus_tag="PC545_000707"
     CDS             43854..44327
                     /locus_tag="PC545_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455497.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYTNFNLNIYSL
                     LIIVCTYGIFNTFISSSNPKKIIIDSKCIHFTSFMTTHKYEIKDIEKIRIREFYNKKI
                     YVKINDGNLFKGRYWIRTNMFNNSIALYSYFIELEECLYPDSLKFRNKKFENKNI"
     gene            44499..45149
                     /locus_tag="PC545_000708"
     CDS             44499..45149
                     /locus_tag="PC545_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2455498.1"
                     /translation="MLLEKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLTPSDIVIVDVDGNIIDGKWKASVDLSDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSKKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            45323..46255
                     /locus_tag="PC545_000709"
     CDS             45323..46255
                     /locus_tag="PC545_000709"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2455499.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVYHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLSTLGLDVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSNKATESNATKSVSSKVTNIKFLLEKDINYFLDYCVEYLKSNIKNIKELELDEED
                     IKSVYNIGEDKYKKIEWTYGKTPKFQVVLPFNNESKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
     gene            46529..47242
                     /gene="ispD"
                     /locus_tag="PC545_000710"
     CDS             46529..47242
                     /gene="ispD"
                     /locus_tag="PC545_000710"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDF2455500.1"
                     /translation="MYSVIIVAAGSGRRMNLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKKLDENCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERNLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            47239..47724
                     /locus_tag="PC545_000711"
     CDS             47239..47724
                     /locus_tag="PC545_000711"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDF2455501.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIESMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    47819..48027
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            48091..49806
                     /locus_tag="PC545_000712"
     CDS             48091..49806
                     /locus_tag="PC545_000712"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2455502.1"
                     /translation="MKMSKMFMPTLKEIPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSAEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNLASQKEEMKELEEVHTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGIVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDKDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAENIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKLRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            49928..51373
                     /locus_tag="PC545_000713"
     CDS             49928..51373
                     /locus_tag="PC545_000713"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2455503.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRVEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAICDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            51813..53294
                     /locus_tag="PC545_000714"
     CDS             51813..53294
                     /locus_tag="PC545_000714"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDF2455504.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHSEGVILDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSVERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALREKIEKTEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            53515..53670
                     /locus_tag="PC545_000715"
     CDS             53515..53670
                     /locus_tag="PC545_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455505.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            53815..55212
                     /locus_tag="PC545_000716"
     CDS             53815..55212
                     /locus_tag="PC545_000716"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDF2455506.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDDFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            55197..55610
                     /locus_tag="PC545_000717"
     CDS             55197..55610
                     /locus_tag="PC545_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDF2455507.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREYLIRQNTQRK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            55716..56474
                     /locus_tag="PC545_000718"
     CDS             55716..56474
                     /locus_tag="PC545_000718"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HDF2455508.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            56487..57224
                     /gene="rlmB"
                     /locus_tag="PC545_000719"
     CDS             56487..57224
                     /gene="rlmB"
                     /locus_tag="PC545_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDF2455509.1"
                     /translation="MASIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEYKYYELDELIDLAKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLDKSK"
     gene            57227..57754
                     /locus_tag="PC545_000720"
     CDS             57227..57754
                     /locus_tag="PC545_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDF2455510.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            57819..58466
                     /gene="sigH"
                     /locus_tag="PC545_000721"
     CDS             57819..58466
                     /gene="sigH"
                     /locus_tag="PC545_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HDF2455511.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            58540..>58820
                     /gene="tuf"
                     /locus_tag="PC545_000722"
     CDS             58540..>58820
                     /gene="tuf"
                     /locus_tag="PC545_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2455512.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNM"
BASE COUNT        22841 a         6987 c        10048 g        18944 t
ORIGIN      
        1 gtataataag ctataaatat taactttaac ttagatttaa gtatatctaa atttaataaa
       61 tataagaaag gttgtgttat tttgcaagat aaaaaaattt atctaaatat tgtactcact
      121 gtagttgttt catatatctt gattaaagtt attgataatt acaaatattt ttttggagtg
      181 ataagtttat taatgtcact actaacacca tttataatag catttgtatt agcatatata
      241 ttcaacccac tagtaaagtt cttagaaagt aaattaaatt ttaagagaat atattcgtta
      301 cttcttactt atggtgtact catagttata ctaatttcgt ttatattatt tacagttcca
      361 tccatagtaa atagtttagc tgatttagtt gctcaaattc ctacatacat agataaaaca
      421 gagcagtttt tattcgactt aggcaagagt ttacaatcag ttgaccctaa tacattaaaa
      481 gagtatggtg acaaaattat gagtgtaatg cctaaattta gtaacttgct tattggttca
      541 cttggtggga tatttagcac tacattttca gttggaaaat ttatagtaca atttctttta
      601 ggttttataa tttgtttcta tatattatta gaaaaagaaa aattcttact gtttacaaaa
      661 aaagtagttt atatttcctt aggtaaaaaa tatggagact ttataataga actatgtcaa
      721 agcttaaatt taaatattgg taaatacttt acaggtaaga ttttagattc ttttatagtt
      781 ggagtacttt ctggaattgg tctttacttt ttaaaatctg aatatgcact attatttggt
      841 acacttatgg gtgtcatgaa tatggttcca tattttggtc cagtaatagg tatggcacca
      901 gttgtaataa ttaatttatt ctccaatcca acaattgctt taacttcttt aatttactta
      961 ataataattc aacaagttga agttacattt atagagccaa aaatcgtagg tggacaacta
     1021 ggtcttagcc cattctttac aatcctagca gttactgttg gtggaggatt ctttggtata
     1081 cctggtatga ttctatctgc tcctgtgatg ggcgttataa agatatactt ctgtgagttt
     1141 gtaaatcgta gacatgataa aatacaaatg gaataaaaat acaatttaat tacgattttg
     1201 taatcaaaag ttacagctgt gtaactgtaa tattattctc tttaatggta atatttaatt
     1261 ataggaaaca tacaatatta ttaagtaatg aatcttatat aattaaacat gaaagagggt
     1321 tttagtatga aaaaagcact tatatcttta ttagttttat cttgtttgtt attaactgca
     1381 ccagtaaatg aatcttctta tgcagattct caaaagaatg ttcctgtaag ccaaaatact
     1441 aaattgactc aagaacaagc taaagaactt ttagtaaaat acaacaatga agttgattac
     1501 atatatcaag gaaatgctag tgattttgaa gcattaaaag ctaaaaatct taatggttat
     1561 gtatttttac cagatgcaga tggtgatatt ggttactttg taaatgaaaa tacttctgag
     1621 atatattact tccacccaag tggatatctt gaattagtta atcaacaaca aaataataaa
     1681 taactaataa aaaatggatg caaattgata taaatttgac atccattttc tttttatttt
     1741 aaagttgttt ctttttataa actttaaata tttttttaat cctgtgcttg ttcttttata
     1801 agtttcttta tatctacagc aggttcatct tccattttta taacttttcc ttcccatgtt
     1861 ctaagcataa cgtaatctgt tccttttgat acaaggtatt gaggaagcaa aggagtcttt
     1921 cctcctcctt ttggagcatt tactatatat gttggtatag ccattcctga tgtatatcct
     1981 cttaaatact ccatgatttc aagaccatca tctactgatg tattgaaatg ttttgttccc
     2041 tttacatgtt tactttggaa tatataataa ggttttactc ttattttcag taattcttga
     2101 tttaaacatc tcattacaaa tttatcatta tttattccat ttaataatac tgcctgattt
     2161 cctaatggaa cgcctgcatt tgctaacttt tcacaagctt ctttagattc cttagttatt
     2221 tccattggat gattaaaatg agtatttata tatacagggt gatatttttt tagcatattg
     2281 caaaattcat ctgtaattct ttgtggcatt gtaacaagag tcctagtacc taatctaaca
     2341 taatcaacat gtggtatctc tttaagttgg ctaagaatcc actctaaata atcatctttt
     2401 aatgtaagag catctccacc agttactagt acatctctta tttcttcatt ttctctgata
     2461 tagtctatag attcttggat aatagctttt gacttatgag aatcttgttg tccaatattt
     2521 cttcttctct gacagtgtct acaatacata gcacactcat ttgttacatt tattattaat
     2581 ctatccgggt atcttctagt tatacatcct gctgggtttg tatactcttc tcccattggg
     2641 tccaaatctt cttgctcatc ttccaactct atatgtgttg gtatagacag taattttatt
     2701 gggtcacata tatcttctgg gtctataaga ctcaaataat atggagatat tgcccatcta
     2761 aattgagtac caacctcttt tatatattct ttttcttttt tagttagagt tataattttt
     2821 gataaagtat ctacatctgt tattctattt gataattgcc atttgtagtt attccaatcc
     2881 tcttcagttc catttaagat acttaatatt ttctgttttc gtttatttat ctcttcttct
     2941 ttttcaagac ctctgttaat cgtttttcta gcctgaatat aatcatcaat ttttgatttt
     3001 aattcagcag ctctctctaa ggatattcta gtttgttcat tcataatttt ttgctatcaa
     3061 agttaactac ttgtattatt tgatagcgtc ctcctttctg atgtgtttat atcattctga
     3121 taggcagatt taattttgcc atatagattt tataacaaat gaaattgaat taatttttgc
     3181 attttttctg ttatttcgca catttatgaa tatttttcaa ccttcgtcaa taaattacaa
     3241 taaattatgc atttttttta acaaatgatt aattttttct atttttaaga attattttac
     3301 cataaaacga ttatattttc aagattatta aaattaaatt ttttaataaa aagtaaagta
     3361 tttcattaaa taataaaagc ggaggtttat tataataaat ttctccgcct ttttatttat
     3421 ttaattaaat aatagtaaaa taaatttaac tcttaagaag tgactttatt atattaagcc
     3481 ctttttctaa ttcttctaca tcttttggag ctgttacaca aatacgaact gcttttggaa
     3541 ctgtagagtt tcctactgaa aatcgttcgg cacaatatac ttgaactcct agattttttg
     3601 cagaaatttc aaatgatttt ccatccaatt catcaggaag aagtaaccaa cgaaaactgc
     3661 aattcttatc accaatcaaa ttataatctg acaaaacttt atcagcaact tcatttcttt
     3721 ctataattgc atctcttttc tcttctataa ttttatctat aataggagaa tataataaac
     3781 ggtgtactat ttctgctgta aatggagaaa ccattagatt catattatat agtgcatttt
     3841 ctaaattttc tatatatcgt tttggagcaa ctacaaatgc tactctaaga ccagcacata
     3901 aaaatttaga tgtactaaaa atatatatag tattatcact agctaaagaa aatatgggag
     3961 tttgtatacc atctctaaaa atgctattta tagcatcttc aataattatt atattatatt
     4021 gctttgctat cttggctatc tttttacgag ataaatcaga catagtatgt gtcgttggat
     4081 tatgataatc tggtataaga tatatgcctt ttatattttc atttttacaa taactttcaa
     4141 gatattcaat cgatatttca ttattttctt gaggaattgg gactaattga atacctatca
     4201 ttttagctat tgacttaatt ccagaaaaac ttaaagaatc tgtagcaatt ctatcaccag
     4261 ctttaaataa tcctagtaaa gttgcacata tagcattttg ccctcctgta gataataata
     4321 tattttcttc actggtgtat acattatttc tttctatcca ttttgcaatt gacttttttt
     4381 gcatatatgt gccagatgga gacatgtatt gtaaaaacct atccatttct ggttgtttta
     4441 gtacattttt aataaagtca ataatatatg tattttgctc atacggagga tgaactgtcc
     4501 ccaactcaat tatgtctttt gattccgttt gatataataa tgtattactt acatttacat
     4561 cagaagaaat gaatgttccc tttcccactt ttgcacatat tagtcccttt tcttcgcaaa
     4621 gtttaaatgc tcgtgttatt gtacttaagt ttaaatctag aaaatctgct aattcccgtt
     4681 gtggaggcaa tttatctcca ggatttaaat ttcctctttt aatatcttct tctaataaca
     4741 ttgctaaagt tttatatatt ggtggttttc ggttatctaa ctttggtttc catgtcatag
     4801 gatagttttc aaaagaattt attggcatat aattactcct cttaattata attgtactcc
     4861 atacaatttt attattgtat gcttctattt tagatgctat acttgtattt gtaaatagct
     4921 atttcaaaat aaataaataa gagaggtata atatgaagac tataggctta attggtggta
     4981 tgagttggga aagtaccatt acatattatc aagttattaa tacagtaata aaagaaagat
     5041 taggtggact acactcttct aaatgtatac tttacagtgt agattttcaa gaaattgaag
     5101 aatgccaatc tagtggaaat tgggaaaaaa gcgccaaaat tttagctgat gctgcaatta
     5161 aattacaaga ggcaggggca gattttattg ttatctgtac taatactatg cataaggtat
     5221 cagataaaat acaagaaagt atacatattc cattacttca catagcagat gtaactgcta
     5281 cggtattaag ggaaaaagaa ataaaaaaag tggccttatt aggtactaaa tatacaatgg
     5341 aacaagattt ttataaaaat gtaattatta ataatggaat tgaagtctta attccaaatg
     5401 aagaagaccg tataatagta aatgacacaa tatttaatga actttgctta ggaattatat
     5461 ctgaatcttc aaaaaaagct ttcttatcta ttatagataa gcttggcaaa caaggtgcag
     5521 aggctgttat attaggatgt acagaaatag gtcttttaat taaacaaaat gacacttcta
     5581 ttccactatt tgatacgaca gtaatacatg caatagaagc cgcactaagt tcaatttaag
     5641 acaatattga ttaataaaaa aaatatctat agatttgtgt aaataagact ttttataatt
     5701 tatattacta gagattttaa acctatatat tgtaacaaaa aaggatagta taattaccct
     5761 atactatcct ttttcttaat ttaatattta aataaactca acactttcaa tttgtataca
     5821 aatctatctt acatttataa catcactatt ttgagagcta tttccattat aatttgcaac
     5881 aacataaata tctagattta aatctccttc aggaatactt atagtatgct catttccctg
     5941 tatatcactt tgatatagag taaattctcc atctccatta gtattatttt ttacatatat
     6001 actgtaagta cagtccttat gagatatact actccagtta actctaatga cattattatc
     6061 tctatcaaca ttagctgata tattttctac cctcatatct ttaatattat tatcattatt
     6121 tgaatttcca tttccattgg taatatttat tacattactt ggcttagttg tatctgaatc
     6181 ctttttacct atgacataaa tatcaaaatc tatattttga ttacccttca tatctacttc
     6241 tatagttgca tataccttgt tagacttacc taaatcactt tcatttatag ttatagattt
     6301 aattttctta aatgattctc ctgacaactt atctttaaga tatatatcat aatcaaagac
     6361 atctttcata taatcccatt caagtttaat aataacttta ttgtttacta ttttatgtga
     6421 accaaccaag ttttgtatat agtcattcgt tatagtttct ttgactactg ttactgttat
     6481 tactttagat ttatctccac aagttattct caccctatct ttaccttctc taagccctac
     6541 aacatttcca ttactactta aacttattgc tctttctaca ggtaattttg aatctactaa
     6601 ctcataatat atttttggtg gttctaaacc aggtggagaa gttattatat ctggtttcaa
     6661 tttttcattc tggtcaactt ctatttttat atcatccata tttatttcag caactctagc
     6721 ttgaatccta aatgttctct caatttcttt atttccacac ttagcatata ttgtagcttt
     6781 accaactcca actgcactta ctttatttcc tttgattttt aaaatatttt cattgctact
     6841 cttataaact ggcttatatg attttttaaa cttatcataa tcaacttcta ttgtactttc
     6901 tatagtagca ctatcaccaa catgttttaa atccccatta atctccatat ctagtgattc
     6961 tacctctggg tctactactt ttatagtagc cttttttaat actcttgaat ttttcatgat
     7021 acccacagaa gaatacccaa cctttaatgc tgtcatttta tttccatcta tttttaaagt
     7081 ccctgagtta tccaatacat taaaccaaat atttggattt ttagcatacg aaggaacctt
     7141 aatatattca cctgtagaca aagtaactgt ttcatttaat tttactacat atttatctat
     7201 atctttaaca tattccccat ctacaatttc aatttctact ccctttacca ttggtctaag
     7261 tattccaaat ttaaaaaggt agtactgcgc aacaagcaat ataggaatag atataattat
     7321 ggcaattttt ctgtatttct tattttttac aaatatcatc tatccacttc cttttagcct
     7381 aatatataat catttgtttt gtaagcaatc tacctaagtt aaatattggg ataaactgga
     7441 aaattattat atttcagaat atccctatac taattataaa ccataaattt aaaatttaaa
     7501 actaaaatta atgttaattt cttactatta caatagtcta attttagtga aataacattt
     7561 gtcttgtcag ctgaaaagtt atataattaa ttaaaaagaa attatactta tattatatga
     7621 ggaggatttt aaattggaaa tcaattataa aaaaagaaat ttcaaatcat ttgctaaaag
     7681 aaaaaatatt gaaatttctg ttcaaaggta tcttattgac gcactaagtt atatggcact
     7741 cggtcttttt tcgtcccttt taattggaac aattttcaat acattaggag ataatttcaa
     7801 tatttctctt tttactgaag ttataagccc tcttgctaaa caggttactg gtccagctat
     7861 agcagtggct attgcgtatg gacttcaagc acctccactt gtactatttt cttgtgcact
     7921 tgttggagct tgtggtaatg aattaggagg acctgttgga gcatttgtcg ctacactttt
     7981 tgctgtagag gttggaaaat tagtttcaaa agaaacaaaa atagatatat tggttacacc
     8041 tgctgtaaca atactaattg gagtacttgt agctgaactt attggtccag ctgtttctgc
     8101 ttttatgact tcatttggaa atataataat gcaagctaca actatgcaac ctataataat
     8161 gggagcttta gtctctgcac ttgttggaat agctcttaca ttacctataa gtagtgcagc
     8221 tatttgtatg atgttaagtt taggtggtct tgctggtggt gcagcaactg taggttgttg
     8281 ttgccaaatg gtaggctttg ctgttatgag ctttagagaa aatggagttg gcggccttgt
     8341 ggcacaaggt cttgggacat ctatgttgca agtacctaat ataataaaaa attggaaaat
     8401 ttggatacca cctactgtag cttcaatatt acttggacca ttatctacaa caatattcaa
     8461 aatggagaat attcctattg gttctggtat gggtacttgt ggattggttg gacaatttgg
     8521 aacagtaact gctatgcaaa gtgctggaaa aggaggtata tctatgtggg ttggtatact
     8581 tttacttaat tttatacttc ctgctattgt aactttgttg atttcagaat ttatgagaaa
     8641 aaaaggtcta ataaaacctg aagatttaaa actagatgta taaattaaaa actactcctt
     8701 ttacttatat aaaagtaagg agtagttttt tttatgaaaa ttccaacatt ttttttaata
     8761 gccaatccat agaaccttta acttcattcc aaatcactat gttttcatca tagtaataga
     8821 cagcttctgc cttttcttcc tctgtaacaa attctctacc aaaagcattt cgccaatcta
     8881 acaaatataa tccaacgtac ttatttttac ttctagcaac cttaatagaa tcatttgcca
     8941 aaaacatact tttattttta aagtaataaa gagctttact gtacatccat gtagcagatt
     9001 tttcttcttt ataaagttca aataatttat ttattgaagt tctatcattt aatctaatta
     9061 atacatttaa taatttgtat cttattccct gctcatcact aggattaatt tctattaaat
     9121 attctaatac ttttttggac tcctttagat tattaatact ttctaacttt ctagcaaggg
     9181 attcaagtgc tatcatatag gctctggtag ataaaccttt ccaaaatcca agttctttat
     9241 cttctttagt aatcatactt ttacctatat ttacagcttc ctccatatat ctaattgatt
     9301 caatatcact attcgcatct tgtgatagct taatatatct tttatatagt atttttttct
     9361 tttctaaatc taaatctaca gactttgatt tggaattggt ctcttttacc caaaattcta
     9421 cattagaaat agctatagta tcttgattta aaaagtcaat actacaaaca tcttttatta
     9481 aagacgattt attatttata gtatttaaag aaacacctat ttgttttgaa aactcttttt
     9541 ttgttatatg tagtggatgc ttcttattgt ataaaagatt ttgttctaaa actatattta
     9601 gtatagctga agaccatata tcagctctag aagaatgaat tatctcttta ttattatcta
     9661 aaaggttttg caaaatttta aagcttatat ctttgtattc ttgatttaaa ttggagttgc
     9721 aaaatctatt tatcttgtcc ttaatttctt ctatcttgcc tgtcatgact cctcctccat
     9781 attttacata tataaaacta gtgctacaaa ctcaagatct tcttctccta tattttctat
     9841 agaatgtttt tcgccatcag ctgtatacac tacatcaccc tcatttactt ctacaaattc
     9901 cccattatca aatactttac cttttccctt tagtatataa tatacttcaa aatctgttgt
     9961 atggtcatgc acaccaattg aacttcctac tggtaaagct actcttttaa atagtcttcc
    10021 ttttccttta agctcttcat tttgtattat gttttcaagc ttaacctttc ctataccacc
    10081 atttaaattt tcttttactt ctacttgtaa ttcagatttc tttcttatca tagctcatcc
    10141 cacctgccaa acaatcaatc taactactaa attggcatat cctttccata aatatgtttg
    10201 tattttaata taataataac atatattatc ataaaaaaat attactgaat acacaaaaat
    10261 gatactattt ttacatatat ttcgacttaa ttaaattatc ttacaagttg acgtctatat
    10321 gaaaaaaaca tatactcata tagtatggta aaattttgtt aaactattta tctggaggtt
    10381 ctcgacatat gtcagtttta aaaacgcaag aaaaacttaa acaacctaga gatttatatt
    10441 tctgctgttc aatatttacc tttgtaggac ttgcatacta tggtatgaaa ttggtattat
    10501 tattgttttt agctgaacaa gtctccaaag gtggtttagg gttaacacca gcagaatccg
    10561 catcaatgct tgcatctttt ttagcatgga cttatttctc tccagttatt ggtggatggg
    10621 tcagtgatag atttttaggt cccaaaaact gtattatact tggaatgcta atggtttcaa
    10681 ttggttattt tctaggatac atagcaaata gtaagatgga tataaacatc atgatatttt
    10741 tatctggatt aggaataggt ttttataaag gaaacttaca tactatggtt ggaaacttat
    10801 ataccaatga tgacccaaga aaagatggag ctttctctat tacgtatatg gctactaatc
    10861 taggtacctt atttggccct ttaatatgcg gtttagttgc taatgaatgg tttgctatga
    10921 aaagcggatt atctataagt atgtatggat atagatatgt atttttattt tcatcaatta
    10981 cggttctaat aggtttattc ttctttatat ttggaaacag aaagttttat aaacctttga
    11041 ataatgaccc tagtaacggg agagagtcta taagaaaatc actagaatat aagaaaatgc
    11101 tagcttcaag acctttaact ttgattgaga aaaaaagaat aatagtaata ttggtgttag
    11161 cttttttcac agtatttttt tggatagcat ataaccaagc ctctatgtct attgcactct
    11221 atacaaagga acacataaat cttactgttg gcaattttca aattcctact tcatggatag
    11281 attcatacaa tggtctcctt tgtgttattc ttggacctat ctctgcttta gtatgggtaa
    11341 aattatctca aactaaaaaa ggcgatttaa gtgtgccaaa aaaaatgagc ttaggtttta
    11401 tactattagc catagcattt ttgtttatga tagtagcagt tatacaaaca ggaacagacc
    11461 ctaattcaat tcacaaagca agtgtatttt gggtagttgg atttttaaca ttccaaagta
    11521 taggagaaat ttgcttttct ccagtcgggt atggtatggt aaataaactt tcgcctgaaa
    11581 agtatatttc attcctaatg ggtgtttggt ttttaggcaa atttgcagca aataaattat
    11641 ctggctatac gcaagctatt atagaccaac ttggtatgtt acaagtcttc atagtgatac
    11701 catcattttt attgatattt ggaattatac tacttataat gaataaaaaa ttagtagaat
    11761 tatcaaatta aaaatttttt aaagttaata aaatatacaa taaaaaatcg aggcattata
    11821 atacctcgat ttttattgta tctagtacaa actatatagc tctagtgtaa acttttaacc
    11881 attctctttc ttcatcagtt aagaaaggtg atattttatc ataaactaac ttatgatacc
    11941 agtttaaata atctttttca tccttattca ttaattctgg tactatacca tctaagtcaa
    12001 taggagctaa cgtaacaact tcaaattcca taaattgacc atagaaattc ttttcagctt
    12061 ttctaactac aatttcattc tctgttctta taccatgaga accttctata tatatacctg
    12121 gttcattagt agttaccatt ccttcttcaa gaactgcact atcaaatctt tcaggtacta
    12181 ctctccatct aaatccattt ggagcttcgt gaacatttag cacaaatcct ataccatgtc
    12241 cagttccaca ttgataatca attcccatat tccacatgca acttctagat agtatgtcta
    12301 agttatatcc tctacaacca tgcaagaact tagccttaga aagatttatc attcctcttg
    12361 ctacagaagt aaagtgtagt tttaattcat cacttatagg tcctaaaact gtagtcctag
    12421 ttatatcagt agttccatca taatattgtc cacctgaatc tactaaaaat aatccttctg
    12481 cctctaattt ataatttgac tctggagttg cactatagtg catcattgca gcatgctctt
    12541 tatatgcagc aattgtattg aaacttggct caaagaaacc ttcttgctct cttcttaagt
    12601 cctctaattt ttgagtagca cttatttcag tgatttccat cttacctaca tttttcttta
    12661 accagtacat aaatttagtg aaagctacac catcttttac atgtgaattt cttatatttt
    12721 ctaattctac ctcatttttt tgagctttaa aaaacattac tggattgaac tcatcaactt
    12781 tctcaacttc acaaggtata ttattgtaaa ttgtataact aagcttagtt ccatctaata
    12841 aaactttttc agttttgtct atatttttta caaattcata cacatcatta tatggcttta
    12901 tttgaacatt ttccttagct agttcattta gtatttcttc atttaactta ctttcatcaa
    12961 caaatagata aacttctttt aaagttatta ctgcataaga aagtacaact ggattatatt
    13021 taacatctcc acctcttata ttgaataacc atgctatatc atcaagagta gttattacat
    13081 gagtagaagt acctttttca ctcatttttt ctcttaatct agctaatttt gagctaaaac
    13141 tttctccaca atatttaaca tctaataaaa atgctttact atctgataaa gcaggtctat
    13201 ctggccatat actatctatt aaatcatact gatattctat ctttatacct tttttagata
    13261 atttttcagc taatgtagct ccttctctag cagatataac tctaccatca aatcctaaag
    13321 ttcctccttc aggaatattt ttatataaat attcatctgt agttgggcaa ccttcttgtc
    13381 ccattttgaa taattttatt gtacttcctt ctaattgaga ttctgcctgt ataaaatatc
    13441 ttccatctgt ccataaccca gcttcgtctt tagtcacaat aaccgtccct gctgaaccat
    13501 taaaccctga tataaattct cttgatttga agtaatctcc tacatattca ctttggtgat
    13561 tatcagaaga tggaatcata taggcatcta tatttttttc ttccataaat tttcttaatc
    13621 cacttaatct atcttttata ttcacaacaa gacctcctcg aaataatagt ttgaataact
    13681 aaatatataa gtatgtaaag tttattacat aaatattttt tgtataattt atataaatct
    13741 tatacactct aattatcaca attataattt taattataat atctatttgt gtaaagcttt
    13801 ttttcataat caaagaggaa attacattta attttatatt taaatgtaac ttcctttatt
    13861 taaagtaatt catttattta caataattcg tcatgaatta tttttctttt gttagtaaat
    13921 ccatagccat taatcttgtt aatacatttc ttattcccaa taatattaaa ccaaatatta
    13981 ttaaaactgt tgctattcct ccaaatatct gtaaaaaacc aagtgtggct gtaaaaccag
    14041 caatctttcc tgataataaa cttgcaaaaa aacttgttag ataccaggct cccatagaca
    14101 acgtagcata cttagctgga gctaatttat tgaacattgc aagtcctata ggagataaga
    14161 ataattcacc tactgttgta aatatataag tcaatactat aaacaatata ctagccttta
    14221 ccattccatc tggaccatta gctaatgtag caaataccat aactataaac ccaacaccca
    14281 ttattatcat tcctaatccc attttaactg gaatagagaa atcacctctt ttggtatgag
    14341 ataatttaac ccatagggta gctaatactg ggcataaagc cacacataat atagcattta
    14401 gtgatgtaag ccatgctact ggcatttcaa atccaaatag agttctattt acattatccc
    14461 ttacataaat cataaaagaa ctttgtgttt gatagtatgc tgtccagaat aagaccacaa
    14521 ataaaaataa aataaccatt gctattatcc tatttttctc caacttagta agtggtccat
    14581 actcaatttt ttcaccttta ttacctttag tcacaggata ttttccgact tcatttaata
    14641 attttggtgc aagaagtgta aatataatca tacctaacat catgcctata ccacaaatta
    14701 aaaatgccat cttatatcca taagatacta tatctccact tgctcctctt actgcaaata
    14761 cattatcaga aagcaatcca catattattg gagctataaa tgctcctaca tttactgcca
    14821 tatagaataa actgtatgct gcatcttttc ttgacacatc agttttagga tatatatccc
    14881 caactatatt tgagcaactt gatttaaaaa aaccatttcc taaaataatt attgatagac
    14941 ctacccaaac cattgcaata gatgtagatg aagcaaatag tacgaaatct cctattgcta
    15001 ttaaaagtgc acctatagaa atacattttt gttgacctaa aactttatct gctaaccagc
    15061 caccgactaa tggtgtaaga tatataagac ctgtaaataa gccaaacatg gatgttgctt
    15121 cttgagtaga aagacctaaa ccaccttgag ctacacttgc tgtaaaaaat aataccaata
    15181 aagctgttaa accataataa ctaaatcttt cccacaaaat tgtcatgcaa ataagccaaa
    15241 aactcttcgg atgaccactt tttttagata cgctttctgt acttgacata aaaaaacgcc
    15301 cccttttgtt ttatagttaa atttaatatt aatattgctt taaccatttt agcacatact
    15361 tataatatca tcttttctat gtttactcaa tagattatcg cctgtatttt cattttttta
    15421 ttattatttt tacaaatgtt tagtaataaa gatgagaagg tatatttaaa atataaagta
    15481 tttaaagttc tgaaatctaa tcatgaagca aaaaattagc atttggttat caataaatat
    15541 aataaataaa aactcataat taatgaaaga ataaattcat gtctaaattt ctataaaaaa
    15601 tttaaattta tacaatttag ggctttaaaa atttaaataa taggtgtaaa ataatagtca
    15661 aagaaagtca aaaataaaaa ggtgtgtggt gatattaatg gcaactatga cagatataat
    15721 tgaaaaattt attaaagatt tgatggaaga agataatagc atacaaattc aaagaaatga
    15781 actagcaaac ttgtttagtt gtgcaccatc acaaataaat tatgtcttaa caacaagatt
    15841 tacaatagat agaggatatt acattgaaag taaaaaaggt ggcggaggat atgttcaaat
    15901 agaaaaaata cgaaaaagta aagatgggca tattagagaa ctactaaatg aaaaaatagg
    15961 aagtcagata tcctataaaa aagcaaaaga attattagaa ggtttaaaag agtcggattt
    16021 gattaacgaa agagaattaa aattaatatt atatgcaata gatgataaat cattgtgtat
    16081 gccaatatat gaattaaaag agaaggtaag gtctaatatt ttaaagaata taataatagg
    16141 attatttagt atagaggagt gataatttat gctatgtcaa aaatgcaata agaataaagc
    16201 atctgtatat tataataaga tagtcaatgg agaaaaaaca gagatgtatt tatgtagtga
    16261 gtgtgcaaaa gagaatacag aaatgaattt taacttagat atgccttttt caatgatgga
    16321 tatattttct aatttaggtt ttcaacctaa aaaagaactt gaggaaaagc tagtttgtcc
    16381 taaatgcaat acaacatata gtgagtttaa gaataatggg agatttggat gtagtgaatg
    16441 ttataatgca tttagtagtc aagtaaatcc aatgcttcaa aatatacatg ggcatataga
    16501 acatacagga aaagcaccta aaaaatcttt ttataagata agtgtagaaa atgagattaa
    16561 agaactaaaa gaagatttag atagagctat aaaaaatgaa gagtatgaat tagcagctca
    16621 atttagagat aaaattaaat atttaaaagg ttctattgac taagattgga gtgatattat
    16681 gaaagagaac atagtcatga aatcaagagt taggctagct agaaatttaa ataactatcc
    16741 atttccaaat aaattagata aagaatgtgc catggaaata atagaaaaag taaaaaatgc
    16801 gtttattaat tctaacttag aacaaaaaga agaatttgat ttctataaaa ttgaagattt
    16861 agaccaatct aaaaaaatgc ttatggttga agaacatata ataagcccag atttagcaga
    16921 aaatgataaa agtgcagtta tagtaaaaaa agataaaact ataagtataa tgataaatga
    16981 agaagaccat ataaggatac aaactatatg tgatgattta aacttagaat atgcatatag
    17041 tgtagcaaat gaaatagatg atttgttaga atcaagttta gaatatgcct ttaacacaaa
    17101 gctaggttat ctaacatctt gtccaactaa tacaggaaca ggaatgagag cttctgtaat
    17161 gatgcattta ccagcattaa gtcaactagg ctatatggat gaactttata aaatttcatc
    17221 acaaataggt atagcaatta gagggatata tggagaacga acagaagcat tgggaaatat
    17281 atatcaaata tctaatcaac ttacacttgg aagaacagag tctaatatta tagaaaatgt
    17341 aagtggactt acaaaagatg caatttcaaa agaaataaaa gctagagaaa tattgcaaaa
    17401 aaaattaggt ataaaactag aagataaaat atttagatcg atagggacac ttgaaaattc
    17461 aagagtaatg agtagcgcag aggcaatgag ccatctatca aatataaaaa tgggaataga
    17521 aatgaactat atagataaac tagatttaag ggctatagaa caacttatga taggtataca
    17581 accagcgcat cagtcaataa tgtataagag tgatgatgtt gagaatagag atataaatag
    17641 agcaacatat attagagaaa ctttggaaaa attaagagga gggatgaatt gtgaacttca
    17701 atagatttac acaaagagct aaaaaggcaa ttgatttagc gtttgaatct gctaaaagtc
    17761 taggacacaa tattgtcgga agtgaacata tacttttagg tcttttaaga gaagaagaag
    17821 gtatagctgc taaagttcta agtaaagttg gatttacaga agcttattta gaaggcaaaa
    17881 tagttgatat ggaaggtaag ggagaagaaa tctcagaaga tatagtatta agtccaagaa
    17941 gtaaacaaat acttgaatta tcaggaatgt ttgcaaataa attaaaaaca aattatattg
    18001 ggactgagca tatattatta gcaattattc aagaaggtga aggaatagct aataagattt
    18061 taaattatgc tggagtaaat gataggactt tagcccaatt aacaattgat atgatgggta
    18121 ttagtgataa aaatcaatac aaagcagaaa atagttacac gggcaaccag aatcaagcag
    18181 aatctaaagt acttgataag tatggaagaa atcttacact atatgcaaaa cagaataaaa
    18241 tagaccctgt aataggtaga gaaaaagaaa tacaaagagt aatacaaata ttaagtagaa
    18301 gaactaaaaa caatccagtg ttgataggag acccaggtgt agggaaaaca gccatagctg
    18361 aaggtctggc tacaaatata gccttaggaa atgttccaga aacactaaaa aacaaaactt
    18421 tatactcttt agagatgggt tcattattgg ctggggctaa gtatagagga gaatttgaag
    18481 aaagaattaa agaagttgta gatgaagttg ttaaaaatgg aaacataatc ttatttatag
    18541 atgagatgca cacaataata ggggctgggt ctactggaga aggctctata gatgcttcaa
    18601 atatattaaa gccagcctta gcaagaggag aaatacaagt tataggtgca actacaattg
    18661 atgaatatag aaagcatgtt gaaaaagatt ctgctctaga aagaagattc caaccagtta
    18721 tggtagatga accaactaaa gaggactcaa taaaaatatt agaaggatta agagataaat
    18781 atgaagctca tcataaagtt aaaataactg atgatgctat aaaggcagct gtagaattat
    18841 caactagata tatatcagac agatatctgc cagataaagc tatagacttg attgatgaag
    18901 ctgcatcaaa agtaagatta aaagaaaata cacctccttc agaaataaaa aaattagaac
    18961 tagaaataga aaatatagat aaagaaaaag aagaagcagt aagatgtcaa gactttgaga
    19021 aagcagccaa aataagagat gaacaaggtc tacttaaaaa acaattagaa gatgttagag
    19081 aaagatggaa caagtcatct aaacattcag atttagttga tggagaagtt attgctgaag
    19141 tagtgggctt atggacagga ataccagtta ataaaattct tgaagaagag gctgatagac
    19201 ttttgagact tgaggaaata ctgcacaata gagttatagg tcaagaacaa gcagtaaaat
    19261 ctatttcaaa ggcaatcaga aggtcaagag caggtcttaa agaccctaat agaccaattg
    19321 gttcattttt atttttaggt ccaacaggag taggtaaaac agaattatct aaggcattag
    19381 cagaagtgca atttggagat gaaaatcaaa taattagaat agatatgtct gaatatatgg
    19441 aaaaacatgc tgtgtcaaga atgataggtt cacctccagg ttatgtaggt catgatgaag
    19501 gtggtcaatt aactgaaaaa gtaagaagaa atccatattc agttatatta tttgatgaga
    19561 tagaaaaagc tcatccagat gtatttaata ttctgttaca aattctagat gatggtagac
    19621 taactgattc aaaaggaaga actgtagatt ttaagaatac tattgtaata atgacatcaa
    19681 atgttggagc atctacaatt ggtagacaaa aaactttagg atttagtata gctaaaggag
    19741 acgaagaaga aaaatctcaa tatgaaaaaa tgaaagaaaa tataatgggt gaattaaaac
    19801 aaagattcag accagagttt ttaaacagaa tagatgatat aattgttttc cactcactaa
    19861 atgaaaatca tatatcaaaa atagtacttt taatggcagc gaaactacaa gagagattaa
    19921 aagaaatgga tataaaatta gaaatgagtg atgaggctgt taaattaatt tctaaatctg
    19981 gatttgactt agaatatgga gcaagacctc ttaaaagagc tttacaaaaa gagttggaag
    20041 atgagctatc tgaagcaatc ttaaaaggta atgtgaaaaa gggaagtaat gtagtagcaa
    20101 aagtcaaaga tgaaaaaata gtttttgaaa ctaaataatt taatagagtt gcattttaaa
    20161 atattgcaaa tataagtttt ataatctaaa caaaatttta agggcaatga atctataata
    20221 attcattgtc cttaattatt ttatttaatc tttacatatt gtctttcaga tatgctaaaa
    20281 ttatagtata gaataaaaaa ttaaggatga taaaagtatg gcaaaaataa aaacaaaata
    20341 tgtatgtcaa tcttgcggat atgaaacagc taaatggctt ggtaaatgtc cagaatgtac
    20401 aaaatggaat acatttgtag aagaaataga ccaaaaaagc accaaaaaag aggtctttat
    20461 aatagataaa tcttcatcta aaccagtcag tataaactca atagaaagta aagaagaaga
    20521 gcggtttaca acagatataa atgagttaga tagagtgctt ggaggaggta tagttaaagg
    20581 ctctttagtg cttgttggtg gtgaccctgg tataggtaaa tcaacacttt taatccaagt
    20641 atctagtaat gttgccaatt taggaaaaac tgttttatat ataactggag aagaatcaga
    20701 atctcaaata aaaatgagag ctaaaagatt aggtattaac tcagaaaatt tatatatatt
    20761 tgctgaaaat aatttaagta taattgaatc ttatttagaa agtgtaaatc cagaattaat
    20821 aattctagac tcaattcaaa ctgtcttcag tccagaaata tcctcggcac caggaactgt
    20881 cagccaaatt aaagaaggta cttcaaaatt catgaaaatt tctaaaaaaa tgggaatttc
    20941 aacgttcata gttgggcatg ttacaaaaga aggttcacta gcaggaccaa aattattaga
    21001 gcatatggtt gatactgttc tatattttga aggagaaaga tataatactt atagattagt
    21061 aagagctgtt aaaaatcgat ttggttcaac taatgaactt ggagtatttg aaatgaggga
    21121 tttaggatta gtagaacttg ataatccatc taaaatttta atctctgaaa aaccaaaaga
    21181 tgtagcaggt tcagtaataa tatctactgt agaaggtacg agaccaatgt tacttgaatt
    21241 gcaagcatta gtttctccta caagttttgg tataccaaaa agaacatcta caggagtcga
    21301 ttacaataga gtaggtatgt tattagcagt attagaaaaa cgtgttggtt tgcaaataca
    21361 aaatcaagat gtctatataa acatagttgg aggaataaag ataaatgaac cctcaattga
    21421 tttagggata gccatttctg tagcatctag ttttagaaat ataccgatag atgaagatat
    21481 agctgttaca ggagaagtgg gacttacagg agaagttaga gcagtaagct ttatagaaaa
    21541 gagaatagct gagtgtaaaa aacttggatt taaaaagata gtagttccta gaagtaatta
    21601 tgatgttgta aaagagacca aaggcataga aatatggcca gtagataact taagacaagc
    21661 tataaatata gtacttggga ggaatcaata aatatggaga attttctaga taataaaaat
    21721 atgctatatg cattaaaaat gatatctcct ggaactccac ttagattagg tctaaacaat
    21781 gtactaagag ctaagactgg tggattaatt gtaattgcaa caaacgaaga tgtaatgaaa
    21841 atagtagatg gaggatttgc tataaatgca gaatattcac catcatatct atatgaatta
    21901 gctaaaatgg atggagctat agttttaagt ggtgatgtaa agaaaatatt atttgctaat
    21961 gcacaactta tacctgacta ttttatagaa acatcagaga caggaacaag acatagaaca
    22021 gcagaaagag tagcaaaaca aactggtgct atagtcatag gaatttcaca aagaagaaat
    22081 gttataacag tttatagagg aaatgagaag tatgtagtcg aagatatatc taagatattt
    22141 actaaggcaa atcaggctat acaaactctg gaaaaatata agacagtatt ggaccaagct
    22201 gtaacaaatt taaatgcctt agagtttaat gatttggtaa ctatttatga tgttgcatta
    22261 gtcatgcaaa agatggaaat ggtaatgaga gttacaagta taattgaaaa atatgtgata
    22321 gaattgggtg atgaaggaac tttagtaagt atgcaattag aagaattaat gggtacaacc
    22381 agaatagacc agaaattaat attcaaagat tataataaag aaaacacaga aataaaagaa
    22441 cttatgaaaa aggtcaaaaa tttaaattca gaagaactaa tagaattggt caatatggca
    22501 aaactattag ggtatagtgg tttttcagaa agtatggata tgcctataaa aacaagaggt
    22561 tataggattc ttagcaaaat acatagacta ccaacagcaa taatagaaaa cttagtaaat
    22621 tattttgaaa actttcaaca aattttagat gcatctattg aagaattaga tgaggttgaa
    22681 ggaataggtg aaataagagc aacatatata aaaaatggac tcataaaaat gaaacaatta
    22741 gtcttattag atagacacat atgatgaatt ttaaatgaat tttactactt ttttcttgaa
    22801 tataatatac actttggtct ataatagata agtataacta gggggtgaag cattgataag
    22861 gaaagtaaca agaatattgt ttattttact tggatttaca ataggtataa caacctattt
    22921 aacattaatg aaagattttg aaatattaac atttggaaaa gaaacatatg gttatatcgc
    22981 agctgtagta gcaggtataa taatagccat tttaggatat cttatagaac catgggttgt
    23041 aaataaagta aaagaaattg ctaaaatagt ggataaagaa ttgtcaaaat atcctcagac
    23101 tgatatattg ttagggtcta tgggtcttat agtaggattt gttattgcgt atttattaag
    23161 tggattagta aatagaatac caatagtagg tggaatacta tccttactat tatatttatt
    23221 cctaggatat cttggaatga aagttgcgct taaaagtaaa aatgatttat ttaatattgg
    23281 aaaactagga agattagcaa atcctattaa ggataaagat aaggataaag aaaataaaaa
    23341 agaagtaaaa gccattcctc caaaagtatt agatacaagt gtaataattg atggaagaat
    23401 agctgatatt tgtaagactg ggtttataga aggaaaactt attattcctg cttttgtttt
    23461 ggaggaacta agacatattg cagattcttc agatgattta aaaagagtaa gaggtagaag
    23521 aggtctggat atattaaata taattcagaa agaattaaat atagaagttg aaataagtga
    23581 gagagacttt gatgatatag cagaagttga tagtaaatta ttaaagcttg ctcaggtttt
    23641 aaatggaaaa gttgttacaa atgattataa cttaaataaa gttgctcaat tccaaggtgt
    23701 tgaagtatta aatataaatg agttagctaa tgctataaaa ccagttgcta taccaggtga
    23761 agatatggtt gttcaagtgg ttaaagaagg aaaagaggct caacaaggtg ttgcatatct
    23821 agatgatgga actatgatag ttgttgatgg aggaagaaaa tatatgaatg agactataaa
    23881 agtactcgtt acttctgttc ttcaaactcc tgcaggtaga atgatatttg caaaacctaa
    23941 aaattaatat gttcaaagaa acctataatt tgggctgttt attttaaata gatggtccaa
    24001 atttatttat ttataacgat aatgtttaat gtattcttaa agatggggat ttaaatcaga
    24061 aagcagggag aatttatgag aaaaaagatt atatctttct tagcaacatt tatcataata
    24121 ttaacatcag caagtcagta tagttttgca gatgatatat caacacgtgg aaaagtaata
    24181 tttatagata tgaatagaac tagtatgagt aatatgttaa gaataaaatc attaagagaa
    24241 gaactagaca atagagggta tattggcttg atgaatataa gaggcgataa aggaagtgat
    24301 gatagaaggt catatgctag tatgggtgct ggtggtagag ctaatgtggc taatgaagaa
    24361 gatattaatt ttgaaagctc gagtaaggat agaaatatag tttttgaatc agctacagga
    24421 aaaagtgcaa aaggtataaa taatttaact ataaataagt ctataaatga aaatttaaac
    24481 tttggagagt atggttctgt attagggtca ttaggacaaa gtttatctga taatggatta
    24541 aaagcatctg tattgggtaa ttcagatata attgaaaatg gtcaacttat aaaaaataga
    24601 aacttatgtc taactgcaat ggatgaatat ggaagaatac ctaatggaaa tgtagatact
    24661 ataaataaaa aagacttatc tatgccttat ggaatatcaa ctgattatga taaattgatt
    24721 gtagagacta aagaaactta caaaaataat gatgtgattt ttgtggagct tggagacaca
    24781 tatagattag atttatataa accaaattta aatgaaaaaa catatgagag tatgaaagat
    24841 aacatagaaa aaaatataga tatatattta aaaaatatat tttctatggt tgaagaaaat
    24901 gacactatct acatagcaag tgcatttcca agtgatttag attataaaaa taaaagaaga
    24961 ctttctccaa taataaaatt aaatggagaa gggaaaggta tattatcatc ttcaactact
    25021 agaagagaag gtattgtaac aaatttagat gttggggttg aaatattaga caactttaat
    25081 ataaaaaatc aaaatatggt tggaagaaaa tatgaactta ttaacagaga tgataataaa
    25141 gaatttttga tggatgaata tcaaaagata gtatctattt caagcataag gtctacaata
    25201 ctgaatggtt ttgtaagtat agttttcttg tcatggatag ttgctatgat tgcaatttta
    25261 tttagaaagc atatatcaaa aaattataaa gaaacaacat tttttatatt aaaggaactt
    25321 ttaaaaatag gaatagtaat gccactgtca tttatgataa caccaataat gaatttcaaa
    25381 acaccattag caatatcttt aggaataatt ataataacac ttactatata tcttataagt
    25441 aaagttttaa taaagaacga tttaaaaaac atgctgtttt ttactggatt aaccatagta
    25501 attatggtta ttgatgctgg gtttggctca tatttaatga aaagcaatgt tatgagttat
    25561 gattgtataa ttggagctag atattatgga gtaggaaatg agtatcaagg tgtggcaatc
    25621 ggtagtgcaa tatttacatt tgcaatttta cttacatata aaaatatacc aaagtggtat
    25681 ataatagttt tttctttggt tatcttaatt acaagtgcat ctccaattat gggagcaaat
    25741 gttggaagtg ctatttctga atgtgttgca tttttactat ttatactatt gatatataat
    25801 gtaaagatag atttcaaaaa aatcgtatta ttaggtatag cagtagtatt tgtattagga
    25861 gtttttgtag caatagatat gattttggga agtaattcac atttaggtat gtttgtaaaa
    25921 gaaatttact ttaatggacc aggagaaata atacaaacat ttagtagaaa gatagagatg
    25981 aatttaaagc tagctcaaac cagtgcatgg gtaaatatac tgttaactgg aataggtgta
    26041 attttagttc ttatgattaa tcagattaga tactttaagc aactgatgga tgagtatcca
    26101 attgtattta aaggatttat agcatcaata gcaggatgtt tagtaactct tttagtgaat
    26161 gactctggga ttgtgtcatc agctacagcc tttatttatg taattgtacc tatgataaca
    26221 ttaagtataa atcttacagc attaaaagaa taagtactgt atgaataata tgtaaatatt
    26281 aatacattaa tttaacttct ttatttacaa aataaagaag aaagtttata aaaaataaat
    26341 tataataaaa aagagtttat ctcaaaattg attattctaa tcaaatttta agataaactt
    26401 tttttattat aaagataaat aacatataac ctcatatttt aggtagattt ttttcttata
    26461 tgtttaggag tgcatttaaa tttttcctta aaaattctgt aaaataattt tgtattagta
    26521 aatccatgag cttctaatat actgcataag gtatcatttg tgttaattaa atctttataa
    26581 atatgagaaa tacgcacatc atttaaatac tctaagtaag tttgtcccat atactgttta
    26641 aattttctac aaaaatattc tggttgtaag tgtgctacct tagcgatttt gttaatagaa
    26701 ataggcattt tgtaattaat ctttgtatat tctaaaactg gttcaaattt tgacaaatca
    26761 agtactgttt ttttcatatt tttattacct acagatattt taaagtttgt atataattca
    26821 aataaaagtt caaataataa gcttgtaaag agcagattag ctgcaggagg agaactaagt
    26881 ttaatttctc tcatgttttc taatataagt ttaactttac ttgaattttt tttatagtta
    26941 ttgtcattgg cattaacctt aaagtcaaaa tagcaatttt taaaatcagg gatatatttg
    27001 tttagaaaag acaatggtac ttgcaataat atagaggtat tttgataaat acatctagtt
    27061 gagtgaataa ttccagaatt aatgaacata cactcattag cttttaaatt ataagtgtaa
    27121 ccctccatgt taatttgtaa atcaccagaa gttatataaa ttatttctaa tgagttatgc
    27181 caatgatttg ctacataact accactatca tcaaatagtt tgaaattaac atctaaatta
    27241 tcaataggtt caattatttc atgtgaaaaa ttatttctgt tcataataat atcctcctta
    27301 taattttaaa ttataaatag taaatttata atgttcaata ttaaaaagat aatatagact
    27361 aataaattag ataataaaca tacttaagaa aacggataaa atcaaatata ataactgtat
    27421 attaaaataa cttaaaaaaa ttattttatt ataagtgcaa aatataccta tttaaataag
    27481 ataagagggg aggattttat gtgtatataa aaggtgtaaa tttaggaggt tggttagttt
    27541 tagaaaaatg gatgacctca agtttatttg aaggcacaga agctgaagat gaatactatt
    27601 taccaagaca gctttcaaga gaggcatatg aatctagaat taagactcat cgtagtgaat
    27661 acatcacaga gagagatttt gcaacgatta aatctatggg atttaattca gttcgtatac
    27721 cagtaccata ctttattttt ggagattgtg aaccattcat aggatgtgtt aaagaacttg
    27781 ataaagcatt tgcttgggca gataagtatg ggcttagtat attgatagac ttacatacag
    27841 ttccaggaag ccaaaatgga tttgataatg gaggaatatc aggtatatgt agctggtcac
    27901 aaaatccaga atatgtagcg tttacattaa atgtattaga aagacttgca aaacgttatg
    27961 ggatgaggca tgaattgtat ggaatccaaa ttttaaatga gcctataaca gaaagaatgt
    28021 ggaacataat gaatgttcca aatcggttta aggctgtaga taaggagatg gcaaggggga
    28081 gtaaacctaa ttcattagag tttttacgtg atttttatat aaaagcatat agggtaatgc
    28141 gtccttatat gagagaggaa aacgttatag tgtttcatga tgcatttgaa ttaaaagcat
    28201 ggaaagactt tatgagagag gaagaattta aaaatgttgt tctagataca caccaatatt
    28261 tgatgttagc tgaagcagat ggatgtgaac aaagtattga tagctatctc aaatatatta
    28321 gagaaaatta tgctaaagat attttacaaa tgcaaaaata ttttcctgtt atatgtgggg
    28381 agtggagctt atttaattca tatgtttgtg gtatagatac aaatggaggt caatcacctc
    28441 taaatgggat tgaatcaaat atagataaat tatcaaaaga cgataaaaga gaattatata
    28501 ggaagatagc aaaagcacag cttgatgcat ggagaaatgg aagtggtcac tactattgga
    28561 attataagtt attactagat acagttaatg aagaaggttg gataggatgg gatagttggg
    28621 acctaggcaa atgtgtagct caagaatggt atcctataga atattaaact aaaattggag
    28681 gaaaagatat gattaaaaac cctatacttc caggttttaa tccagatcct tgtatttgta
    28741 gaaagggaga tgattattat cttgtagttt catcttttga atggtttcca ggtatacctg
    28801 tttatcactc taaagactta aaaaattggg agttatatac tcatattttg acagatgaaa
    28861 caaaaataga tttgaagaag cttccttcat ctaaaggaat ttgggcaccc tgtttgactt
    28921 attgtgaaga agaagattta ttttacatag tttatggaat tatgaactct atgaatgcaa
    28981 gatattttga tgttgataat tatttaatta catcaaaaga tataaaggga gagtggagtg
    29041 aaccagtata tctacactca tcaggttttg atgcgtctat ttttcatgat gatgatggta
    29101 aaaagtggat tgcatcatta gactgggaga ctagagaagg atatgaaaaa cctggtgtta
    29161 tatgtttagt ggaatactgt acaaaaaaga aagaaatagt aggatatcca aagagaatat
    29221 ggtcaggggg aactgataga ggttgtattg aagcaccaca tataacaaag cgtggagatt
    29281 actattatat aatgtgtgca gaaggtggaa ctggatatgg tcatagtgta actatgggaa
    29341 gggctaaaaa tatatgggga ccttatgaaa aagattcaat gaatccaatt gtaacttcaa
    29401 ttccaggcga tttttatgaa agacatgacc ctgaccatct aaagcctaaa tactataatc
    29461 cagaatcaaa acttcaaaaa tctggtcatg gaagctatat agaaacaaca tcaggtgaag
    29521 tatatttagt tcatctaaca tctagaccat ttgtaccaga gttaaggtgt actttaggta
    29581 gagaaacagc tattcaaaaa atgaaatgga caaaggataa ttggcttcga atggaagatg
    29641 aaagtaattt ggcaaaagaa tacgtatcag aaagtaaact agaagaacat ttagtttcat
    29701 ctataccaag ttttgatgac tttgattcaa atgaattagg tttacagtac tatgctccta
    29761 ggatttcacc attatcattt gcagatgtta agtctagacc aggatatgta aggattagag
    29821 gacaagaatc aagaacatca ttaaataaag taagcatatt ggcaagaaaa ttaacttcag
    29881 tatatgcaag aataaccact aagatggagt tttacccaga ggtacatcag catagtgcgg
    29941 gtttgataat gtattatgat aatatgaatt atataaattt aagaaagtat tatagtgaaa
    30001 cattgggtca aagtgcacta tcaataatac atcttgaaaa tggagagaaa acggaatttt
    30061 taaatactag gataccaata aaagatatac caatatatct tcgtctgtat atacaaggtc
    30121 gtaagtcata ttttgaatgg agttatgatg aaaaaaacta ccagagaata ggaaaggtat
    30181 ttgatacaac taagttttca gatgagtact gtaaatatgg agaatttaca ggtacgttta
    30241 taggattaac ttgtgcagat cgtgtaaagc ataaacacta tgctgatttt gatttttttg
    30301 aatatatagt agatgaatca aaagatgtcg attaataaaa tcattactaa aattttcaga
    30361 gcaaaggtaa atcttatgct ctgaaataag aaacattaga aggcaaatct tacaaaaagg
    30421 agtatggtat tatgaataca gaagttaatg ttaaagcaac tacgattaga tatgaaaaga
    30481 tttcattttt tgaaagaatt tcttatggtt gtggggattt ggggtgcaat atcatatact
    30541 cagctatgtc agcatttttg ctgttttatt atactaatta tgcagatgtt agtgcagcag
    30601 ctgtaggtag tattatgctt gtatctagga tactggatgg gtttagtgac ttaacaatgg
    30661 gaattatagt ggatagaact aaatccaaat acggtaaggc taggccttgg attttgagaa
    30721 tggcgattcc ttttgctata gcagcagtat tattattttc tgttccatca aatcttggta
    30781 tcacttctaa attaatttat atttttatta catataactt agtttctact gttatttata
    30841 cagcaatcaa tgtaccctat gcaactctaa attcacttat aactcaaaac cagtatgaaa
    30901 gaggagtatt aagtatattt agaatgatat tagccacttg tggtacctta ctaataaatg
    30961 gacttacact acctctagtt gaatactttg gaaataattt atcagcatgg actaaaacat
    31021 tctttgtatt tggaatagca tctataatgg tattttttat aacatttact ggaaccaagg
    31081 aaagagtaaa agcagttaag caagatagga atgaagtaat accatttaaa ataggtatta
    31141 aatctttatt tagaaataag tactggatac aaataacatt atgtttagtt tgcatattta
    31201 ttgtatttgc gataaatggt ggttcatccg tatattatgc aaagtttatt ttgggagatg
    31261 aaaaattatt tggtcctata aatatggttt caaatatatc ccaaattata gctatgttta
    31321 tggttgctcc ttttataaaa aaatttggaa aaagaaatgt attaatagta ggttcaataa
    31381 tattaatatc gtcaaatgtt atgtttataa tagttggaca aaatcatata ggagtaatat
    31441 ttgcaagtgc tataaaggga ataggaagtg ctggaatagc atcaacaatg tttgctattg
    31501 tttcagatac tattgaatat ggagaatgga agactggata tagaactgag gggcttataa
    31561 atagtgcatc tagttttggg tttaaagtgg gaaatggatt aggttcagca atattaggtg
    31621 cagtattatc aattggtggt tatgtaggaa catcagctac tcaaagtgat ttagctatag
    31681 tatcaataaa agtatgtttt atatacctac ctatatttat aacaatatta caaattatca
    31741 taatgtcttt ttataagctt gataaagaat atagtactat acttaatgaa ttaaatacta
    31801 aataatgaaa aagtttctta taaagtctta agtttaatta aggtctattg taaatgttcc
    31861 ctataaaaac tattatatat ttatgattat tgaagaattt ttagagaagc gaataataaa
    31921 aatataagaa gactttaaat gatgaaatgg ttggtttttt agaaatgatt taataagtta
    31981 ttgttattta aagcattata agtggacaat taatttttaa tttatggaga tgatatttaa
    32041 caattagaag taatatataa tatgcactaa gaagcaattt ttcaactatt tatgaaggtg
    32101 gtggtacaag aaaagtatca cctttttttt gttatacaaa aaaacagttg actttttata
    32161 tgaacccctt ctataatgtt actgaaatta agtaaaatga ttgaacaaat gtaaagataa
    32221 ttgaaaagct tgattttgga ggaaagcaaa tgaaaaaaat tggagactta agattaatga
    32281 tgaagtgttg ttcactttat tacgaagata atttgaatca acaggaaata gcaaatcaat
    32341 tgggaatatc aagaccaaca atatcaagaa tattaaaaga agcttttgaa caaggaatag
    32401 taaaaataca gatagttgat gttttgaaaa atgattacca aaaaatagag agaagtttgg
    32461 agagaaaata taaattaaaa gaagtaatag tagtagatga taaacaggat gcactaacac
    32521 aaaaacaaga actagcaaga gctgtatctg aatatttaac tagagtagta aaggaaaatg
    32581 atataatagg tgtttcaata ggaacaacac taaaagaaat accaagatat gtggaaaaaa
    32641 gtaactgtaa aaatgttaca tttatacctc tattaggagg gattggagac aatgaaatag
    32701 atattcatgc aaatcaaata gccgtaagtt tagcgagggc ttttggaggt gattttaaat
    32761 tattacatgc tccagctgtt atgtctgact tgtctacaaa agagaaactt tgcaaagacg
    32821 aaaaaattaa agaagtacta gatttaatag ataaaacaac tatagcaatt gtgggtatag
    32881 gaaatcctat gagtttaaac tcaacaataa tggcttctgg atacatgaat gaagcagata
    32941 tagaagattt aaaaaaatac aactcaatag gtgcaatttg tttacaagcc tttgataaag
    33001 aaggaaaaac ttctatactt gaatttaatc aaagagtatt aggagtgaaa ctggaagatt
    33061 taaaaaagat aaaaagaaca ataggtgtcg cttcaggaga tgaaaaaata gaggcaataa
    33121 aggcatcatt aaaagcgaaa tttataaata gtttagcaat taatcacagt ttagcactta
    33181 aattattaga agattgtgac taatagaaag atagataaaa gttaacagga gggaatgtat
    33241 atgtcaatat caaaagaaac attgcttgaa atgtataaga gaatgaatca ggctagaaaa
    33301 tttgaagaga aagttagttg tttttttgca agaggtatgg ttcatggaac aactcactta
    33361 tctgtaggtc aagaagcgtc aagtgtggcg gctgtaatgg ctttagaaaa aggagattta
    33421 gtatcattaa ctcatagagg acatagtcaa tttataggaa tgggaataga cctaaataaa
    33481 atgatggctg aacttatggg taaagaaact ggattttgta agggaaaagg aggctcaatg
    33541 catatagcag atattgagtc aggtaactta ggagcaaatg gagttgtagg tggaggtctt
    33601 acaatagctc caggagctgc acttacacaa cagtataaaa aaacaggcaa aatagtttta
    33661 tgtagttttg gtgatggagc atcaaatgaa ggtacttttc atgaaggaat aaatttatct
    33721 tcaatatgga aattgccaat aatattttac tgtgagaata atttatacgg tatgtcaaca
    33781 agcataaaac gtcatatgaa tatagaaagt attgccacta gagcagcttc ttatggaata
    33841 gaaggaatat caatagatgg atataaccca atagaagtat acgaaacagt acaaaaagct
    33901 gctgagaagt gtagaagagg agaaggacct gtattaattg aaagtagaac ttatagatgg
    33961 ttaggtcatt caaaatcaga tgcaaatgta tatagaacaa aagaagaaat agaatcatgg
    34021 aaagcaaaag accctattga atttttaaaa aattatctta tagaaaataa tctatctaat
    34081 gaagatgaat tagataagat tcaagaattt gcaaagcaat caatagaaga tgcagtagag
    34141 tttgctcaaa atagcccaaa tccaaaaata gagtctttat tagaggatgt atatgcagac
    34201 taagaatggt aaaggaggaa aataaaaatg agtacaaggg aattaacata tgcacaagca
    34261 ataaaggaag caatgtcaga agaaatgaga agagatgaaa atgtaatatt tatgggagaa
    34321 gatataggaa tatatggagg agcatttggt gtatcagttg gtatgataga tgaatttgga
    34381 ccagaaagag taagggatac tcctatatca gaagcagcta tagcaggagc ggcagcagat
    34441 gcagcagcaa ctggcttaag acctattatg gaagtaatgt ttatggactt tgtaacaatt
    34501 tctatggatg caattgtaaa tcaagcggca aaaatgagat atatgtttgg aggaaaagca
    34561 caagttccaa tggttgttag atgcccagga ggttctggaa ctggttcagc agagcaacat
    34621 tcacaaagtt tagaagcttg gttttgtcat gtaccaggag taaaagtagt tgcaccttct
    34681 acaccagcag atgctaaggg attgttgaaa gcagctataa gagataataa tccagtcata
    34741 tttgttgaga ataaattgtt atacagaaaa aaaggttttg tgccagaaga tgattatgta
    34801 attgaaattg gaaaggcaga tattaaaaga gagggtactg atgttacagt aattacttat
    34861 ggaagaatgt tacaaagtgt tgaagaagca gcagagactc tatcaaaaga aaatataaat
    34921 gttgaaatta tagatttaag aactttatat ccattagata aagagactat agtaaagagt
    34981 gtatgtaaaa ctgggagagt tttaatttgc catgaagcag ctaagactgg tgggctaggt
    35041 ggagaaatat cagccctgat aacagaaagt gaatcatttg attatttaga tgctccagta
    35101 aaaagaattt gtggaaaaga tgtgccaata ccatataatc cagagttaga aaaagcagtt
    35161 gtcccaagag ttgatgaaat agaagaagca ataaaatctt taattgttag ataaggaggg
    35221 atagatatgg tggcaaataa aataaaagcc acaccagcag ctagaagtca ggcaagaaaa
    35281 gacaatataa aattagatag gctgattgga agtggagaaa atggaaggat tcatttagta
    35341 gatgtgttaa attatttaaa agataataaa gcaaatacta ctcctttagc tagaagaata
    35401 gcagaagatt taaatataga tttagaaact attgtaggta ctggatataa tggaaagata
    35461 agaaagtgtg atgtggaaaa attaactgga aaagaaacta ttgtatcaac taatacatct
    35521 aaatctagtg agaaaaaaga gctgaaaatt gaaaatgaaa attcaagaat gtttaacaca
    35581 gtagaaggaa tatttgaaaa gccaaatcca atgagagcta ctgtagcaaa acgaatgtca
    35641 gaaagttatt ttagtgctcc tgtatttaca ttcaatatag aagtagatgc tacagaattg
    35701 aaggtactta gagcaaagtt aatcgacaca gtaaaagaat caactggagt aaaattaact
    35761 atgacagatt taatagtaat ggcagtatct aaaatacttc caaatcatca agccttaaat
    35821 tcagcatgga cagatgaggg tatatttaga tataaagatg taaatatagc tattgcagtt
    35881 ggtttagatg aaggcttata tgttccggta gtaaaaaatg caaacaaaaa gtctttaaaa
    35941 gaaatagcaa aagaaagcaa agaacttgcg gaaaaagtaa agacagggaa acttatgcca
    36001 gcagaccaag aaggtaatac atttacaata agtaatgttg gaatgtatgg tataactaca
    36061 tttacaccta taataaatat gccttcaagt gcaatcttag gggtaggtgc tacacaagat
    36121 aagtttgttc cagtaaatgg agaggctaaa ataaaaccta taatgaactt atcattaaca
    36181 tcagaccata gagtaataga tggaacagta gctgcaaaat tcttaaaaga tttaaaagaa
    36241 ttgttagaaa atcctttatc aatgcttgta taggttttga tattttgtta ctgatataaa
    36301 tagtttgaat agattatatt attataattg tttcagtaaa tttaaagctt ttatatatta
    36361 agaattattt ttaacagtaa ctatatgtga atatgatttg cttaattaaa atatatgaaa
    36421 ataaatagga ggagagccta tgtcagtaga agtaataatg ccaaaagcag gagtagccat
    36481 ggaagaaggt actatagtat cttggctaaa gcaagaaggc gaagaagtaa agattggaga
    36541 acctatacta gaaataacaa cagataaagt taatatggag atagagtcag aaggagaagg
    36601 tacacttgct gtaatcatac ataaagagga aggtgaagta ttaccagtat ttacagtaat
    36661 aggtgtaata gcagaaaaag gggaaaatca agaggaagta aaagctaaat atttatctgg
    36721 aaatgtttca aaagaagata tagttgaaga gaatcaaaat atagaattta aagaagaaaa
    36781 aataaacaaa aaagagtgta atcatgatta tgatgtagtg gtaataggag gaggacctgg
    36841 gggatattta tcagcattaa aagcagctct tttaggtgga agagttgctt tagttgaaga
    36901 aaatatacta ggaggtactt gcttaaatag aggttgtatc cctacaaaga cttatataaa
    36961 aacagcagaa atattagaag aaattgacca attatcaaag agaggtgtaa aagtaactgt
    37021 agataaagaa caggacatta agaaggccat aaaatataaa aatagagtag ttaaaaaact
    37081 tacagctggt gttggcggat tattaaaaag tagagatgta gatgtgttta atcttaaagc
    37141 tagtgttaaa gaagagcata aagtaatttt atcagatgga aaagttttag atacagaaaa
    37201 tataataatt gctacaggtt ccaaagtaag gatattacct ataaaaggaa tagaatcaaa
    37261 tttaataata actagtacag aagcattaga tttggaaaca gtgccagaag aattagtaat
    37321 aattggtggt ggagtgattg gttgtgaatt tgcagagata ttcaattcaa gaggttctaa
    37381 agtaactata gtagaaatgg aagatagagt aatcccaaga atggataaag agttaagcga
    37441 atctttaaaa tattctctta gtaaaaaagg tataaatgta ttaactaaaa agaaagtttc
    37501 tgaatttaaa gaagaaggaa ataacatatt agtgtgcatt gaaggtgaag agccaataaa
    37561 agcagattta tgtctatatg ctataggaag agaagctaat ctttctggca tagaggactt
    37621 agatattaaa atagataaag gttcaatagt tgtaaatagt aaaatggaaa caagtatacc
    37681 aagcatatat gcagtaggtg atgttactgg aggtgtaatg ttagctcatg ctgcttttaa
    37741 aatgggagag gtagcagctt caaatgctct aggtgtgaat aaagaagttg acttaggtgc
    37801 attacctagt tgtgtttata caattccaga agtagcatca gttggtatta cagaagagga
    37861 tgctagaaaa aaatataatg taaaagttgg taagtttaat tttgctggaa atggaagagc
    37921 tttagcttct ggtcaagaac aaggatatgt gaaggtagta gcagatgcta aatatggaga
    37981 aattttaggt atacatatgt ttggatgtgg tgtagcagaa cttataaatc atgcagcatc
    38041 atttaaagcg ttagagatac ctactgatga agcaagtgag ttgatatttg gtcatccatg
    38101 tacttcagaa gctttaatgg aagctttggc agatgtaaat ggagaatgtt tacacttacc
    38161 taaaaaataa atctaaacat aaattattaa tttaagtcta ttattaatat taatttatat
    38221 cataaaattt taaaacactt acttagccat accccttatt atgcaaataa tttatttgca
    38281 taataagggg tattttttat ggaaaaatat agaatataga atccttgtag tttttgttgt
    38341 acaaaaaaga tagtaatata aaataacatt atcattttag aatgatttca attgtaatta
    38401 ggaaaagtga tttttttgtt tttaaaaaaa gtattatttg taaaatataa aaaaaaattt
    38461 cttaatattt gaataaaaga atagcttaat ttaaatgtta ttactttata aaaaaataat
    38521 aagtacaaaa aaaacaatat atggcatata agttgctata taaataataa ggagggaaca
    38581 attataaatg aggcaaagaa taaaaaactt aattgaaaat gaagataaga aaaatccttt
    38641 aacagatgaa gcgatttcaa gtaggttaaa tattcgaaga gaagatgtta cctttttgag
    38701 aaatgagtta aaaatagaag attcaagaca aagaagaaaa gttgttttaa ttaaagctat
    38761 aaaagatatt ttaaaagaag aaaaaactat aaataaaaat gatataacaa gaagattaaa
    38821 cagcattggg tttacagtat caagatttac agtcatacaa tacttaaaag agattcaaga
    38881 ttctggtgac ctaaaacttg ataaagagag tgatgataaa aaagaagaaa taatcaataa
    38941 tagagaaaat aaagatatag catttgataa acttataggt agtagtggta gcttaagtac
    39001 aattataaaa ctagccaaag cagcaattct ttatccacct catggtcttc atactattat
    39061 tatggggcca actggtgttg gaaagagtga gttagcagag tgtatgtata aatttgcagt
    39121 agaatcatcc agattcccta agaatgcacc atttatagtt tttaatgcag cagattattc
    39181 agagaatcca cagcttttgc tatcacagtt atttggacac gttaaaggtg cttttacagg
    39241 agctgatgag aacaaagaag gattagtatc aaaggcagat ggaggaatat tgtttataga
    39301 tgaaatacat agattacctc atgaaggtca agaaatacta tttcaactga ttgataaagg
    39361 tatggtcaga aagttaggag aaacaagact aacacataaa atagatgtaa tgattatttc
    39421 agcaacttct gagccaatag attctcattt acttaataca tttaaaagac gtatacctgt
    39481 aacaattgaa ataccagaat tagttgcaag accattaaat gagaggtttg acattataaa
    39541 taatttcttc ttaatagaag ctcaaagaat gggagcaaac atacatataa agtcagatgt
    39601 attaaaagct cttatgcttt acgattgtat aggtaatgta ggtcaactta gaagtgatat
    39661 tcaagtagct tgtgctaggg gactattaaa ccaacttaca aacaaactaa aagaagtaaa
    39721 tataagcata tcagatgtac ctggatatgt aaaacaaggt cttataaaaa tcagaaattg
    39781 tagagggaaa atagaaaatt atgtagatgg ggatttaatt atagactcaa atcttcaagg
    39841 tgagatagaa aagaaaaatg aagatatata tacttttcca gatgaaatat acaaacttac
    39901 tgaaagaaga catattgatt tattggaaca aggtttagat tttgatgtta taaatagaat
    39961 cattggagga gaattagaaa gttgcattca aaaatacata aaacaagtta aaaaagtttc
    40021 tattagtaac aatcttccag atataagtcc aattgttgga aaatatatcg tagagctaac
    40081 ttatgaagta atcaaaatag catcacaata cctaagttca ctagaaccga atctacattt
    40141 atgtttagca gttcacctaa aagcagcttt tgagagatta aaagaaggaa aagtaatatc
    40201 aaatgctcat ttgaaagaaa taataggaaa ttatccagta gaatattctg tagcagaaaa
    40261 ggtagctcaa tatttaaagg aaagatataa cataaactta ccaaaagaag aaataggtat
    40321 aatagcaata tattttaaaa tgagtagtag aagagatatg tctgaacaaa aaaagatagg
    40381 cgtacttctt atggctcacg gaagagtagc atcttctgta tgtgaggtta caaataaact
    40441 attaggaatt aatcacgcaa ggtatttaga tatgccattt gagaagaagc cggaagaaat
    40501 gattgaagaa gctactacaa taataaaaga tattgatgaa ggtagaggtg tgattatatt
    40561 agtagatatg ggttcacttt catattttgg agatattatt tctggaaaga ctggaattaa
    40621 tataaggaca atatctagat ttgatacatt gctttctata gaggttatac gaagagcagt
    40681 tttaccagat ttaacaatag acgagatagt tgatgaagtt aaaggtatag agccacaagt
    40741 aaaagaaaag gttagtaaat gtaacaatac aaaagtagat tttagaaaac ctataatttt
    40801 aacattatgt attactggat tagggggagc ttgtaagata aaaaaaatct tagaggaaaa
    40861 tattaaaagt ataactgaaa aggttgatat agaacctata ggtatgattg aatatccaag
    40921 tcttgattta gagataaaac aaataagcaa tgataaaaat gtaatcgcca tagttggaac
    40981 aattgaccct cagatagcaa atatcccttt ttttagtcta gtagacataa atgataaagc
    41041 gaagttaaat caaataaata acctaattga tacaaatcaa attggtgaaa caaataaaaa
    41101 atatgaatta tcagatttaa tacataaaga agatatattt gtggatttac aggttaaatc
    41161 aaaagaagaa gttctaaaat atatgagtaa aaaacttaga gagaaaggtt atgtgacaaa
    41221 aaatttctat aaaaaagtag tggaaagaga taacttattt ccaactgaat tatcagatga
    41281 aatagcaata cctcacacag atggaataga tgtaattagg ccagctataa gtatagccat
    41341 cttaaagaag cctattgtgt ggagtaaaag taaagtaaaa ataatacttt tattagctgt
    41401 agaccaaaag tgttttaatc cattaagcac tctactctca tttatagaaa cagatgaatt
    41461 taagaagata aaaaacatta aagatagaga ttttattagg gagatgatat tagatggagt
    41521 caatgaaaat aattagtagc aatttaattt ttaaaaatat tgaagtgtct aacaatgaag
    41581 atgcattaaa gtttttggga caacgtttgt ttgacgaaca gtatgtaaaa gaaagttaca
    41641 tacaagctgt tgtagcaaga gaaaaaaagt atgctacagg gttaccaaca gaaatatacg
    41701 gagtggcaat accacataca gatatagtac atgtaaatga gccaggaata gcaattggaa
    41761 tactgaataa acctgtaaaa tttataatga tgggtacaga cgatactgaa attgatgtaa
    41821 aagttgtatt tatgttagca gtgaaagagc cacaagaaca attgcaacta ttagaaaaac
    41881 ttatgactat atttcaagat aagaatatac taaataatat tgttgactta agtgaagaaa
    41941 gtgtgagtga tttattaaac tctaaattga aaaattaaac atgtaaagta tattaaaaat
    42001 gtaaaattaa acatgcaaag tatgttaaaa aatataaaaa gattattaaa gatttctaac
    42061 tatagatatc tttaaaataa aataatttca atgggggtga ctaatgtggc taaaaagata
    42121 ttagttgcat gtggaacagg agtatgtact tcaacaattg ctataaacaa attaaagaaa
    42181 gcacttcaag acattggtaa attggatatg gttaatatta cacaatgtaa agtagtagaa
    42241 gttgcatcaa aagcacctga ttatgattta ataatttgta ctacacaagt atctagtagt
    42301 ataaaaacac cagtgataaa tggattacct tttttaactg gtgtcggaat ggataaatta
    42361 atcaatgatg tattagaaga gttagaatta taattaaaaa aattaaggaa catggtctct
    42421 aaaatcataa gttttagaga cttaaaatta aataatggag ggtctttata tggagatatt
    42481 aaatttcatt gttggtctag gtgcacaagt aatgatgcca ataataatat gtatttttgg
    42541 tttgatatta ggtacaaaac taggaaaatc tttaagagct ggtttaactg tgggagttgg
    42601 gtttataggt cttaacacta ttatagcttt gctaacagat aatttaggtc cagctacaca
    42661 acaaatggtt aagaatttag ggctaagttt atcaataata gatgtaggat ggccagcagc
    42721 atcagctata gcttttgcat caacagtagg ggctttaatt attccaatag gtcttgtggt
    42781 aaatatagtt atgctaatta ctaatactac tcaaactgtt gatgtagaca tatgggatta
    42841 ctggcatttt gcttttacag gagctttagt ggcaggtgct actcaaagtg taatgtgggg
    42901 agtatttgca tcagtagcaa atatggtaat tgtattggta atggcagatt taactgctcc
    42961 aggtatagag gaatatttag gtatgccagg tatatctctt cctcatggat ttacacaggc
    43021 atttgttcca attgctatag tagtaaataa attattagac ctaataccag gtataaataa
    43081 gattgagata aatgctgata cattgcagaa aaaatttgga ttatttggag aacctttaat
    43141 aatggggtct gtaataggtg taataattgg tattgctgcg aaatatgata taaaaggaat
    43201 attacaatta ggtgtaacta tgggagctgt attaatatta attccaaaga tggcagcatt
    43261 attaatggaa ggattattac cagtatcaga agctgcacag gaatttattg agaagagatt
    43321 taaaaacaga ggcaagattt atataggctt agattcagca gtaggaatag gtcatcctgt
    43381 tacattatca gttgcactag tgttggttcc tttgactata cttatagcag ctatattgcc
    43441 aggaaataaa gttttaccat ttgcagattt agcagtaatt ccatttgcac ttgtattaat
    43501 cgtaccaatt acaaagggaa atgtgtttag aacattaata ataggaataa ttataattac
    43561 ttctggatta ttaatcgcaa ctaacttagc tccattattt acccaaatgg ctttaaatgc
    43621 tagttttaaa atgcctgaag gagcaactat gatttcaagt atttgtgatg gtgcgaatcc
    43681 attaagttgg gtatttgtaa aagttatgaa ctataaagtt attggaggag tagtttttgg
    43741 agttatagct ttaggaatgg ctatttacaa taggaataga ataataaatg aaaataaaaa
    43801 gctatctata gaagaataag gttaaaacaa ttttattaag gagtgtatag gttatgaaaa
    43861 aatataaata tgattctaaa agatacttac tcagtgttac aataccagga ttttttatga
    43921 ttttaatatt gatttatgca ctgtttaata actatacaaa ttttaatttg aatatatata
    43981 gtttgttaat aattgtctgt acttatggta tattcaatac ttttatatca tcaagtaatc
    44041 ctaaaaaaat tattattgat agcaaatgta tacattttac ctcatttatg actacacata
    44101 agtatgaaat taaagatata gaaaagataa gaataagaga attttataat aaaaaaattt
    44161 atgtaaaaat taatgatgga aaccttttta aaggtaggta ttggataaga acaaatatgt
    44221 ttaataatag tatagcatta tatagctatt tcattgagtt ggaagagtgc ttatatccag
    44281 attcactaaa atttagaaat aaaaaatttg agaataagaa catataaaaa taaaaaattg
    44341 aattgaagcc tagcttaatc ttttttgata agatataagt ttgaacttta tactaaattt
    44401 ttagaatttt caatcaaaac aataacataa tagctaagca attagaacac aattaagatg
    44461 tataaggcat gtatatgatg aatgggagga tttaattaat gttattagag aaattaagaa
    44521 aagaagtatt acaagcatct ttagaccttt taaactataa tttagtgaca ttaacaggag
    44581 gaaatgtaag tgggagagat gaacaaactg gctatatagc aatcacccca agtggaatgg
    44641 attatagaaa tcttacacca agtgatattg ttatagtaga tgtagatgga aatattatag
    44701 atggaaaatg gaaagcatca gtggatttat cagaccactt gtatatatat aagcatagag
    44761 aagacataaa tagtataatt catacacatt ctacatactc aagttgtttt gctattttaa
    44821 atgaacctat agaatgtgca agtacaactc ttgcaaatga agtagggggt tcagtaccag
    44881 ttgcaaaatt ttcaccacca acatctaaaa agatgggaaa atgtgttatt gaagcaattg
    44941 gtgacaaaag agcatgtctt ttagcaaatc atggcgtaat tgcagttggt ccatctgtag
    45001 gacatgctct tacagccgct gtaatgttag aagattcagc aaaagtatat tacttagcaa
    45061 aatcaattgg cacaccagtg ctactgcctg atgaagaaat tcaaagagcg agagatgtat
    45121 tttttaatgt ctatggtcag gataaataaa ttagataaaa ttttaaaagt aatatgaaaa
    45181 tatagatatt caatgatatt aaggagattt ataatgatat aataagttta atagttaaga
    45241 agtaaaatat gaaatgtaat agtatataaa ttcatttaag actaaaaaga ataagataaa
    45301 ataaaaagga gtctgtattt ctatgtatta cttattaaac aaaagcacaa atccttactt
    45361 taatttggca ttagaagaat atctattttt aaatgataaa tataatgatg atattataat
    45421 catatggaga aatgaagaat ccatttttat aggtaaaaat caaaatccat atcaggaagt
    45481 atatcatgat gttattgaaa agggagaaat tcctatttta agaagaatat ctggaggagg
    45541 aactgtttat catgacttag gtaatatcaa tatgtcattc atacaaaaag ataggcaact
    45601 tcatgagata gattttttgg agcatactaa gtttatgcaa aagatgcttt caaccttagg
    45661 actagatgta tctataactg aaagaaaaga tttattttta aatgggaaaa aaatttcagg
    45721 ctctgcacag tctataaaaa gaaaaaattc tctttatcat ggaactcttc tttacgattc
    45781 agatttaaat aaattaacta aatatttaaa ttcaaataaa gcaactgaat caaatgcaac
    45841 taaatctgtt tcaagtaaag ttacaaatat taagttttta ctcgaaaaag atataaatta
    45901 ctttttagat tattgtgttg aatatttaaa atctaatata aagaatataa aagaacttga
    45961 attggatgaa gaagatatta aaagtgtata caatatagga gaagataaat ataaaaagat
    46021 agagtggaca tatggtaaaa cgccgaaatt tcaagtagtg ctaccattta ataatgaaag
    46081 taaaataaat atacatgtta acagatggaa agtatcaaaa ttttttattt cttatgatga
    46141 taatgtaatt aatatggaca agttgttaga tttgaatttc tttaaagaag aaataaaaga
    46201 gagtatttta gaaaattatc cacattacat agatttaatc aatttaatat tttaatataa
    46261 aacctctaat ttgataaaat tgtcttatat caaactagag gtttttaata ttcttgcata
    46321 ttaacaacca ttacaaaatt gtaaaattaa aaacaacact attgaaaaat caatatatat
    46381 tgtaataaat atatgtgttt ttcattagca aatagacatt aactatttat acaactgaaa
    46441 atttaatata taattaatca gatggttaaa ataaaaatat taaaagtttt aataaatgat
    46501 ggttaaaaat aaaatacgag gtgataccat gtatagtgtt ataattgttg cagcgggaag
    46561 cggaaggaga atgaacttag atataaacaa acagttcata aaattaagag aaaaagaaat
    46621 tattgctcat acaattcaag ttttttatga aaatataaat atagatgaaa tagtggtatg
    46681 tataaaaaaa gaagaagaag atttttttaa agaaaatata ataaataagt ataactttaa
    46741 aaatataaaa atagcatatg gcggaaaaga aagacaggac tcaatatata atggtttaaa
    46801 aaaattagat gaaaattgtg atatagtact tatccatgat ggagcaagac catttgtcga
    46861 ccatagaata atcaatgaat ctataaaggt agctaaagaa aaaaaggctg tggttgtagg
    46921 tgttccagta agtgatacta taaagatagt gtcagatgga acagtacaag aaactccaga
    46981 aagaaatttg ctgtgggcag cacaaacccc tcaaaccttt gaatataatc tcataataga
    47041 tgcttatgaa caagcatata agaacaatta ttatggtact gatgattcaa tgttggtaga
    47101 aaatataggt caaagtgtca ctatggtaat gggttcttat gaaaacataa aaataactag
    47161 tccagaagat ttaaatatag cagaacaaat attaaatatg gaaaaaagag atgaagttaa
    47221 cagtagaagg agaattatat gagaatagga ttaggatatg atgtacacaa attaacagag
    47281 gatagaaaac ttataatcgg tggtgtagag ataccacacg ataaaggttt gttgggtcat
    47341 tctgatgctg atgtattgat acatgcaata atggattcta ttttaggtgc tctagcatta
    47401 ggagatatag ggaagcattt cccagataca gatgaagaat ataagggagc agatagtatg
    47461 aaattattag aacatgtgta taatctaata acaagtaaag gatataaaat tggtaatata
    47521 gacagtacta taattgctca aagccctaag atggctccat atattgagag tatgagaagt
    47581 aatatatcaa aagttttaaa cacagacatt gataatataa acataaaggc aacaacagaa
    47641 gaaggtcttg gttttacagg agcaaaacag ggaatagctt cacaaagtat ttgtttatta
    47701 ttgttgacaa gtcagaataa ttaatatatt attagtaata agtttaatgg atatcaagta
    47761 ataggcctat atagaatatc tataatgtat gaatttaagt gaaattaata taaatataaa
    47821 attagctatg ataaagaaat agtaaaatga tgaaatcttc acagagagaa aacggtggtg
    47881 agagttttca agaggaagtc tttgaacctg tcttttagct taaaggtata aaaatcttta
    47941 cggtaggaac cgttatattc ccaaaagaga gctattcata tagctaacta gagtggtacc
    48001 gcggaaatta aacctttcgt ctctatactt gtatagagat gagaggtttt ttatattttc
    48061 aggaagttaa tattcaggag gaatctatta atgaaaatgt caaaaatgtt tatgccgaca
    48121 ttaaaagaaa tcccagcaga tgctgagatt acaagccatc aattaatggt taggtcaggc
    48181 atgataaaaa aaatgacttc aggtgtttat aaccaattac caatggggtt aagagtgttc
    48241 aaaaaaattg aacaaataat tagagaagaa ttaaataaaa aagattgtca agaaatactt
    48301 tgtgcagcct tacttccttc tgaattatgg aaagagtctg gaaggtggac tgctatgggt
    48361 gaagaaatgt ttagactaaa agacagaaca gaaagagagt actgtcttgg gccaacacat
    48421 gaagaagcat tcactgatat aataagacaa gaaattactt catataaaca attgccacta
    48481 aacttgtacc aaatacaagt aaaatataga gacgagagaa gaccaagatt tggtgttatg
    48541 agaactaaga cttttacaat gaaggatgca tatagttttg atgtagatga taaaggttta
    48601 gataagtcat atcaagatat gtttgatgca tatgtaagta tatttgatag atgtggattg
    48661 gagaatagcc ctgtacaggc agattcagga gctataggag gttctacatc agccgaattt
    48721 atggttaagt cagaagttgg tgaagatgaa gttgtatttt gtagtggatg tgattatgca
    48781 gccaatgtag aaagagcaga atcttgtaat ctagcatcac aaaaagaaga aatgaaagaa
    48841 ttagaagagg ttcatacacc aggagcagct acaataaaag agttagaaga atttttaaaa
    48901 acttctccag ataagtttgc taaaacttta gtatatgagg cagatggaaa aacagttgta
    48961 gtcgttgtaa gaggagatag agaagttaat gaaatcaaag tatctaatgc cataggttct
    49021 gttatagaat ttgctcttgc tacagatgat gtagtgagaa aagtaactaa tgcagaagta
    49081 ggatttgcag gtccaatagg tataaatgct gactatgtat ttatagataa agaaattgtt
    49141 gaacaaagaa acatagttgt tggggctaat aaaactgagt accatataaa aaatgctaac
    49201 tatggaagag attttgaagg aatagttgga gattttagaa atgttcaaga aggcgacaag
    49261 tgtatagtat gtggtaagcc acttgaaata gctagaggtg ttgaagtagg acatatattt
    49321 aaactaggaa ctaagtactc agaatctatg aatgcaaact tcatagacaa agatggaaaa
    49381 tcaaagccta tagtaatggg atgttatgga ataggtgttg aaagaacagc agcagctata
    49441 attgaacaac ataatgatga gaaaggtata atatggcctt tgtcagtagc tccataccat
    49501 gttgtgatag ttccagcaaa tatgaaaaat gaagaacaaa tatcaatagc tgaaaatata
    49561 tataatgatt tgcaagctat gggtgtagaa gttttattag atgacagaga tgaaagaata
    49621 ggtgtcaaat tcaatgattc agaattaata ggaataccta tgaggattac agtaggtaaa
    49681 aatattaatg aaggtaaagt agaatttaaa cttagacata aagaagataa agagataatt
    49741 gacatagaag aaataaatga aaaagtaaag gcagagttta tcaggaataa tgttaggtta
    49801 ggataataac ttaaataaga tattgtatag ataatcaaag ttgtacttta tagacaccat
    49861 tttatctata tataaggata taaaatggtg tttttttata aaacaaataa atgaggaggt
    49921 aattaaaatg gcaaaaaatg aaaaacaatt tgttgaagaa ataacaaaga tggaagatga
    49981 ttttccacaa tggtacacag atgtaataac taagactgac ttagttgatt atgcaccagt
    50041 aaaaggtttt atggttgtaa agccatatgg atatgcatta tgggaaaaga tgcaagaatt
    50101 tatggataaa aaatttaaag aaactggaca taaaaactgt tattttccat tattaatacc
    50161 agagagttta ttaaacaaag aagcagaaca cgttgaaggt tttgctccag aagttgcatg
    50221 ggtaacacat ggaggaaata aaaaattaga agaaagatta tgtgtaagac ctacttcaga
    50281 gactataata tgtacaatgt atgcgaaatg gttaaaatca tatagagaat taccatatct
    50341 ttataatcaa tggtgctcag ttgttagatg ggaaaaatca actagacctt tcttaagaac
    50401 atctgagttt ttatggcaag aaggtcatac gttacatgaa acagcagaag aagcacaaga
    50461 agaaacaata caacaattag aagtatataa agcgctatgt gaagaattac ttgcaatgcc
    50521 agttgtagct ggtcaaaaaa gtgaaagtga aaaatttgca ggtggtgaaa gaacatatac
    50581 tatagaagct atgatgcatg atggtaaagc attacaatca ggtacaagtc acttcctagg
    50641 tcaacacttt actaaggctt ttgatattac ttttgcagat agagaaggaa atttagctaa
    50701 tccatatcat acatcttggg gagcatctac aagacttata ggtggactta taatgactca
    50761 tagtgataac agaggtttag tattaccacc aagagtagct ccaattcaag ttgttatagt
    50821 tcctatagct gctaaaaaag gaaatgtaat ggagactgtt gataaaatat atgctgattt
    50881 aaaagctaag ggagtagctg tagaagttga tgatagagat aactatacaa ctggatggaa
    50941 atttaatgag tgggaaatga aaggtgttcc agtaagagtt gaaattggac caaaagatat
    51001 agaaaataat caagctatgg tatttagaag agatacttta gaaaaggatt ctatgccttt
    51061 agaaggatta gctgatgcta tatgtgattt atttgatgtt atacacaatg atatgtttga
    51121 aaaagcaaga aaacatagag aagacaatac atctatagtt gaaaatatgg atgagtttag
    51181 aaaagcttta gaagaaaagc caggattcat aaagactatg tggtgtggag atgcagaatg
    51241 tgaagctaag attaaggaag aaacaggagc aactataaga tgcttacctt ttgaacaaga
    51301 aaacttaggt cacaagtgtg tgtactgtgg aaaagaagca gatagtatgg tagtaatggc
    51361 aaaagcttac taaaatacat ttaaataaaa actggggtag aaagtattta atactttatc
    51421 ccagtttttt tgttttttat gagtaataaa atgtatttgt aactatgtca taagataagt
    51481 catagatact tatattaaaa agaatataat agagaactat ttttaataat tacatattaa
    51541 tgaaagcaaa atatttttgt tatatagatt ttcaataaag caaacaataa atataaatga
    51601 atattagtaa ggagtgcttt taatttttag aaaataaatg acttaaaaga aagatttatg
    51661 aaattaaaac taggattttc tgatataata aacatgtgta tttttaaata aaaaggatat
    51721 tattattaga tttgattaaa aataaacttg caaaacaatg tactagttta atattatagt
    51781 tatattgaat aaaatatcag gaggaagtaa aaatgaatgt aagagtaagg tttgcaccaa
    51841 gtccaacagg atttgtacat ataggtagtt tgagaactgc tttatataac tatttatttg
    51901 caaaaaaaat gggcggagaa tatatattaa gagtggaaga tacagaccaa agtagattag
    51961 tagaaggtgc tatagagaat atgcttaatg ctatgaaatg ggctggggtc aaccatagtg
    52021 aaggtgttat attagatgat agtggaaaag tagtacaaaa aggagaatat ggtccatata
    52081 ttcaatctca aagattagat atataccaag aatatataaa gcaactttta gatagtggaa
    52141 aagcttatta ctgtttctgt acaaaagaaa gactggatga agtaagagat gctcaaagag
    52201 cagcaggaga aacagctaaa tatgatggtc attgtaagaa tctttctaaa gaagaagtag
    52261 aagcgaatat aaaagcagga ataccatatg ttataagatt aagattgcca gagaatcata
    52321 caattaagtt tacagactta gttagaggag atatggaatt taatactaat gacttagatg
    52381 accaagtatt aatgaaaaca gatggatttc caacttatca ttttgcagta gttgttgatg
    52441 actatttgat gaaaattaca catgttataa gaggagaaga atgggtatca tctactccaa
    52501 aacatgttta tttatatgaa gcttttggat gggaagcacc tgtatttgtg catttaccaa
    52561 atatacttaa taaagaaaag aaaaaattaa gtaaaagaca aggtgatgta gctgttgaag
    52621 actttaagaa aaaaggatat ttaccagagg gattagtaaa ctatgtagca ttagttggtt
    52681 ggtctcctga agataatcaa gaattgttta caatggagga attagaaaaa gccttctctg
    52741 tagaaagagt ctctaaaagt ggtggagtat ttgatactga aaaattaaat tgggttaatc
    52801 agcattatat aaaagatggt gatgatgctt atctaacaga cttagcaata ccatttttaa
    52861 tagaagatgg atttataaca gaagaagaag ctactaataa atatgatttc ttaaagagta
    52921 tgatatcagt acttaaagag aaattacaat atgtaaaaga agttactgaa catgcaagta
    52981 tattttttgg agataaaata gaggtagaaa ctgaagaggg aagagagttc ttaagattag
    53041 aacatatacc tactttaata gatgctttga gagaaaaaat agaaaaaaca gaggtattaa
    53101 atgctgagtt tgttcaagct atgcttaaag aaatacaaaa agagtacaaa ataaaaggga
    53161 aaaatttatt tatgggctca agaataattt tgacagggca aatgcatggt ccagatttac
    53221 caaaagtaat ggaagtttta ggaaaagaaa cttgcttaaa tagaatagct tatgtaaaaa
    53281 ataatatatt atagtcataa aagtccgcta atttagaagc ggactttttt tgtgttagta
    53341 tttggtgaaa atatctgctt aagaaaatga aggtatttaa ggtaataaat ttaatatata
    53401 aaaagcaaag caaaaatata aataaaatat gaataataaa ctaataaaat attaagcata
    53461 gaagaaggga atctattatt ttagtttgat ttaattatta agggggtaat tactatgtat
    53521 agtaatattg atgatgttaa aaaggaatta aaggagctat gcttagaata tgttacaatt
    53581 ttagaaaaat taaaagatga aaaaatgatt acagaagaaa cttttgaaaa gtgctcttct
    53641 caaaaaaaaa tatttttaga agaacaatag aggatggtaa tagtattatt attatgtagt
    53701 ttgaaagata gtacgaaaaa tgatagtaat actattattt attgcaattt tcaactataa
    53761 tataatatat aatgttaaga agataattta taaaaataca gtgaggtgat gagtgtgaaa
    53821 gtttataata cattgactag aacaaaagaa gaatttgttc cattagaaga aggaaaggta
    53881 aaaatgtatg tgtgtggtcc aacagtttat aactatatac acataggtaa tgcaagacca
    53941 tttataatat ttgatactct aagaaggtat ttagagtata gaggatatga tgtaacttat
    54001 gtacaaaact ttacagatgt tgatgataag attataaata gaagtcatga ggagggaata
    54061 tctcctgaag aagttgcggc taagtatata aaagaatatt ttgtagactg tgatgggcta
    54121 ggaataaaaa gagctactgt tcatcctcag gttacagata atatacaaca gattatagag
    54181 tttataaaag agttggaaga taagggatat gcatatgcag taaatggtga tgtatatttt
    54241 gatacaaata agtttgaagg ttatggaaaa ctttcaggtc aaaaacaaga agatttagag
    54301 gcaggggcta gaattgaggt taatgaccaa aaaagacatc ctatggactt tgttctttgg
    54361 aaggcaaaaa aagaaggtga gccaggatgg gatagccctt ggggagaagg tagaccagga
    54421 tggcatatag aatgttctgt tatgtctaag agatatttag gtgaaacaat tgatatacat
    54481 gcaggtgggc aagatttaac attcccacat catgaaaatg aaatagctca aagtgaagct
    54541 agaagtggaa aaacattctc aaaatactgg atgcataatg gatatataaa tataaatgat
    54601 gaaaagatga gtaaatcaaa aggtaatttc tttacagtaa gagatatatc aaaattatat
    54661 gatttagaga tagtaagatt tttcatgtta tcagcacatt atagaaatcc agtaaacttt
    54721 agtgatgaga tgttaaatca agctaaagca ggtcttgaaa gactatataa tactaaagaa
    54781 aaattagaat ttacgctaag taatttagta gaatctcctt taacagaaaa agaagtagag
    54841 cttgttaaag aattagatga ctttagacaa aaattcatag atgctatgga tgatgatgtt
    54901 aatacagcag acgcagtaag tgtaatattt gaattagcta agttaataaa ttctaatgta
    54961 gatgaaaatt cttctttgga gtttgctaaa aaatgcttag atgaatttaa tgaacttaca
    55021 ggagtattaa atattgtaaa taagaaaaaa gatactgtat tagataaaga cattgaagaa
    55081 ttgatacaaa aaagaactga tgcaaagaaa aataaagagt ttcaattagc tgatgatatt
    55141 agacaacaat tactagatat gggaattgta ttagaagaca caagacaagg tgttaagtgg
    55201 aaaaggatat aaaaatggaa aaaactgaat tagttactat gtcaccatta gttttagcat
    55261 acttaggtga tacagtgtat gagacatata taagagaata tttaattaga cagaatacac
    55321 aaagaaaagt taatgatttg cataaactag ctataaagta tgtaaaagct aaagctcagg
    55381 caactataat tcatgaaatt gaaattgagt taactgaaga agagagtaaa atttataaaa
    55441 ggggaagaaa tcaaaagtca aatacttctc ctaagaatgc agatattata gactacaaac
    55501 atgcaactgg gtttgaggca ttagtgggat atttgtattt aaataatgaa atagagagac
    55561 ttcaatatat tatcaataaa ggaatcaaaa ttattgaaag agatatgtaa atattaaaga
    55621 gtgttgtctt aataataagt aataaggcaa cacttttttt ataactaatg tataataaat
    55681 atatagaaat tagtaatgag aaaggataaa aagttatgaa agtaaaatta atatcacata
    55741 cacctgaacc agaaaaagta atagctatgg cagctaagct atgttattca ccagttggga
    55801 cagacgaaat agaaaaagac ttaacagatg aaagtataga gaaattttta aatatgttat
    55861 taagtatagg tcatgggtca atattagaac atgcttcatt tacattttca atagaaggta
    55921 tttcaagggc ttgttcacat caaattgtta gacatcgtat agctagtttt tcacaacaaa
    55981 gtcaaagata tgttaaatta gaacaatttg agtacataat tcctccagaa atagaaaaaa
    56041 tagaaaaggc aaaagagtta tttatagact ccatgaaaaa agaccaagag aattatgata
    56101 agttagttga aatattgttt gaaaatcatt ataatgacct aataaaaaac ggtaaaaatg
    56161 agaaaacagc taaaagacaa gctgaaaaga aagcaataga agatgctaga tacgtatttc
    56221 caaatgcatg tgaaacaaaa atggtattta ctataaatgc tagaagtttg tttaatttct
    56281 ttgaacatag atgctgtgaa agagcacagt gggaaataag aaatttagct gtggagatgt
    56341 taagagaggt taaaaaggtt gctcctatat tatttaaaaa gaccggtcca agttgtgtaa
    56401 atggaagttg tccagaagga actatgactt gtggagatat agttcaagtt agagaaaaat
    56461 ttaaggcctt gtaggaggtg aataatttgg cgagtataga aggaagaaat ccggtaatag
    56521 aagcaataaa aagcgacaga gaaatagata aaatattaat tgcaaattca gctaaagaag
    56581 gttcaattaa gaaaataata ggaatggcta aagataagaa tataattatt cagtatgttg
    56641 ataaacataa gctagatgaa gtaagtacaa gtcattcaca tcaaggtgta atagcttatg
    56701 caagtgagta taagtattat gaattagatg agctaataga tttagcaaag aataaagatg
    56761 aagacccatt tttcattata ttagatgaga taacagaccc acataatttg ggttcaataa
    56821 taaggacagc agatgcagta ggagcacatg gagttatcat tccaaaaaga aggtctgtac
    56881 acataactcc tgtagttgcc aaagcatctg ctggagcagt agaatatatg ccagtttgca
    56941 aggtaaccaa tatagttaat acaataaaaa gactcaaaga agaagggctg tggattgctg
    57001 cagctgatat ggatggagaa actttttata aacaaaatct tactggtcct cttggtgtag
    57061 taattggaag tgaaggtttt ggaatatcta gattggttaa acaaaactgt gactttatag
    57121 taaaaatgcc aatgataggt aatgttacat ctttaaatgc atctgtagca ggtggaatac
    57181 ttttatatga gatattcaga caaagactgg ataaaagtaa ataggcatga aaaaaaatat
    57241 aaatcattac ttaataatag atggctacaa tattataaat gcgtgggaca atttgaaaga
    57301 actggctaaa gaggacttag aagattctcg agaaaagctt attgatgaca ttatagaatt
    57361 ttctgagttt atgggttata agactataat agtttttgat gcttataatg ttaaaaattc
    57421 tcgagaaaaa gttgaaaaaa gaaaacacat aacaatagtt tatacaagag aacaccaaac
    57481 agcagatagt tatatagaga aatttataac ttctttatca aagtatgatg atgtaaaagt
    57541 agccactaat gactatgcag aacagcaaat gattttagga aaaggtgcta ctagaatgtc
    57601 tgcaagagaa ttaaagttag agttagatcg ttccaagaat aaaatgaagg aaaaaaatat
    57661 cggtttgagg aaaaaaattc agcgaaattg gttggaggaa aggttagata aagaaacatt
    57721 gtcgaaactt gagaacattc gtagaaagcg ttgaaattac taggttcttt agactataat
    57781 atacctataa atttgtttta tatatggggg agatttacat gttggtagca aaagaaaaaa
    57841 gttatgagtt agtggacaat tgtcagcagg atgagtataa cattgtatta agagcaagtg
    57901 aaggggataa aatagcacta gagtacatta ttactaaata cagaaatttt gtaaaggcaa
    57961 aagcaaagtc atatttttta attggagcag ataaagaaga tataatacaa gaaggaatga
    58021 taggccttta taaggcagtt agagattttg atggaagtaa gaccaattca tttaaatgtt
    58081 ttgcggagat atgtataaca agacaaataa taactgctat aaaaactgca acaagacaaa
    58141 aacatatacc tttaaattca tatgtttctt tgaataaacc aatatatgat gaagagtcag
    58201 acagaacact tttagatatt atagctacaa gtatagtaac agatccagaa gagcttataa
    58261 ttagtaaaga agagttaaaa aatatagaat caaaaatgaa cgaattgtta agtgacttgg
    58321 aattagaagt actagaatta tatttaaatg gaaagtcata ccagtttata gcggacaagc
    58381 ttaaaagaga tgtaaagtca atagataacg ctttacaaag ggttaagaga aaattagaaa
    58441 aacatcttga aaacagaaat gattaatagt aaaatactta ccataagctt attgtgagca
    58501 aaattaattt atatcgaaag ataagggagg aaattcaaaa tggctaaagc taaatacgaa
    58561 agaacaaaac ctcatgttaa tatagggaca ataggacacg tagaccacgg taaaactaca
    58621 ttaacagcag caataacaaa aacattatat gacagatatc aattaggaga agcagtagat
    58681 ttcgcaaaca tagataaagc tccagaagaa agagaaagag gaatcacaat atcaacagca
    58741 cacgttgagt atgaaacacc aaatagacac tatgcacacg ttgactgccc aggacatgct
    58801 gactacgtta agaacatgat
//
LOCUS       DAMJVI010000010        56784 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.010, whole genome shotgun sequence.
ACCESSION   DAMJVI010000010 DAMJVI010000000
VERSION     DAMJVI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.010"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(202..1041)
                     /locus_tag="PC545_000723"
     CDS             complement(202..1041)
                     /locus_tag="PC545_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2455513.1"
                     /translation="MSDIKNEVSKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAITGIL
                     GLF"
     gene            complement(1065..1823)
                     /locus_tag="PC545_000724"
     CDS             complement(1065..1823)
                     /locus_tag="PC545_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2455514.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNDNVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(1870..2343)
                     /locus_tag="PC545_000725"
     CDS             complement(1870..2343)
                     /locus_tag="PC545_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455515.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSYNGDENLIVLVKYPKTVLGLVNSGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELISKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(2372..2788)
                     /locus_tag="PC545_000726"
     CDS             complement(2372..2788)
                     /locus_tag="PC545_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2455516.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAIIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLKL"
     gene            complement(2835..3887)
                     /locus_tag="PC545_000727"
     CDS             complement(2835..3887)
                     /locus_tag="PC545_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2455517.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGKEVRSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDGDGADIVIESAGTPLTCGQVLLLAKKGGTVLYAGVPYGDVALTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSAGLYHMQKGDMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIDN"
     gene            4283..4963
                     /locus_tag="PC545_000728"
     CDS             4283..4963
                     /locus_tag="PC545_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDF2455518.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSVKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(5096..5416)
                     /gene="sugE"
                     /locus_tag="PC545_000729"
     CDS             complement(5096..5416)
                     /gene="sugE"
                     /locus_tag="PC545_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDF2455519.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            5798..6625
                     /locus_tag="PC545_000730"
     CDS             5798..6625
                     /locus_tag="PC545_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDF2455520.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGSKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGALIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGIAICYDIRFPE
                     LSRLMALKGAEIVILPAAFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGRIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            7428..8102
                     /locus_tag="PC545_000731"
     CDS             7428..8102
                     /locus_tag="PC545_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455521.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNGYEVYTAQSGKGALKKISK
                     NPDIILLDINMPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGADDYIVKP
                     FDLDELGARVSAHLRREKRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETVWGV
                     GYKWIK"
     gene            8090..9250
                     /locus_tag="PC545_000732"
     CDS             8090..9250
                     /locus_tag="PC545_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2455522.1"
                     /translation="MDKINNMSLKKAFFVLTLSSLIIASTLTTFSYILLNNLYNSIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIENLDENISIIAKQEGKSFTLKSQINNINLF
                     FDENIILRVVENIISNAFRYARNNVSILIYLEQELLTFVIEDDGIGFSKESLKLALRP
                     FYRDKTLNDSNAHFGMGLYISKILCEKHGGSISIENNSTNSAKITAKFSTRN"
     gene            9521..9994
                     /locus_tag="PC545_000733"
     CDS             9521..9994
                     /locus_tag="PC545_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDF2455523.1"
                     /translation="MLSFTFCSLPFWICYFWCGISDIFDGLIARKLRQQSGIGAKLDS
                     IADFIFSVTILVTVGKNIALPMYLWWCIVAIALLRLISYSIGFFKYRTFSSLHTLMNK
                     ITGISIFAFPLLYICFGLDVAGIMICIVAFLSSFEEIAIMIKSKELNRDIKSIFM"
     gene            10280..10948
                     /locus_tag="PC545_000734"
     CDS             10280..10948
                     /locus_tag="PC545_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455524.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSVIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHTEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKEYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            10951..13419
                     /locus_tag="PC545_000735"
     CDS             10951..13419
                     /locus_tag="PC545_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455525.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDRHVSFHQLTKNQVDDIKKDDRVYQAGTSITLGSSKIKDSGISVLV
                     KEYDKTGLSNYPKLMKLKSGNLPKDKNEIALDENTLKLMGIKPRLGVTIPMNLDISLL
                     NDTIPPYNYTANFKLSGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIK
                     QQEKFQEIVNQLAKKINLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLF
                     VALLVLLASGLVIYNILKISVTKKIKEYGCLRAIGAEPNQIYKIVILQILILCTIAIP
                     IGAVIGIISSKGITGMVTNILNPDILLANDNKEITELIHKNTTAYIFPLVLSTSISLI
                     FSLISALPSAIYASHVSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRT
                     IITILSLFMSITVFVALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDKM
                     KNVNRTSFIKYSEYKQGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSILEDFK
                     NGSICFIKNPIAISTPGVKTRHTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWING
                     VDVIVYDSVYNTLTNKNKFNQINIYAKDKSKLEQIRLSIEQICENNPGSHWISYIESD
                     KQLKESFKQIEFLAWAVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLY
                     KMFLWEGVYYGIFATIFGSIAGYVSAIIINMATIEKLDFTNIPITSILQATIISILAC
                     IIATLIPLRKVKKMNIIDCIDVNL"
     gene            complement(13658..15082)
                     /locus_tag="PC545_000736"
     CDS             complement(13658..15082)
                     /locus_tag="PC545_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455526.1"
                     /translation="MKNQSLITQFRHTFIFIIIASIVATVITYVFALYLYIHSLNKDI
                     YPPNYYERQVPRIEKYINEKNITLLSQSNEEGLKRTIRGDDMLYQVVDNNGNILYGTN
                     PKKLFKTKEELFNNFINKTVRKGGYIHTVPIKGDNGKIEGAVILFYQVKITFANIRGR
                     FVFAVIIMALFSPFLYIVGFTRWLSKRFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKGSLSAQWKMEQERVEMVEALAHDLKSPLSIILGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNLVPINLPEFLRQ
                     KVQDYELQAHQKEVELILKMQGNIQSPIQIDVDRLTRIFDNIISNSLQYTPSGGNISI
                     TVKDEKNCISYEICDSGRGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(15101..15793)
                     /locus_tag="PC545_000737"
     CDS             complement(15101..15793)
                     /locus_tag="PC545_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455527.1"
                     /translation="MSKILIIDDEMDLVMLLEDELKAKGHEVLIAYDGQAGIELSKQE
                     PDLIILDIMMPKMNGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFGKLCLYMKERAIKIDGKNIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            complement(15848..16624)
                     /locus_tag="PC545_000738"
     CDS             complement(15848..16624)
                     /locus_tag="PC545_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2455528.1"
                     /translation="MIKLLSSEWLRAKRTAVQWLTLFMPIIFSLCVVFYLTTKSGSTQ
                     EFAFEGFFTVWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMSIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGEKVWAFVPWTWPVKLGMLPGIYFIKEAGTISTEA
                     FYSKVMQTASIELIVVAIGLIIFLIGGVIWFKMWEGRKSY"
     gene            complement(16621..17391)
                     /locus_tag="PC545_000739"
     CDS             complement(16621..17391)
                     /locus_tag="PC545_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2455529.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QTTSWAWQSLLALIFNWWSFLFLPIGFALFATLVAFQEKKAGNYRALRTHNVSPMTLW
                     INKVIAMAVYSFISTLVLIVVTIITGLILKAGPVPFGQIIGASIVCWVVSLAILPLQL
                     WLATWKGMFLSMGVGALGMIFGVLAATKPFWIAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            complement(17388..18092)
                     /locus_tag="PC545_000740"
     CDS             complement(17388..18092)
                     /locus_tag="PC545_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2455530.1"
                     /translation="MKDLILETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTNGRIIFDRKPWKRESLAKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLSDPELLILDEPTNGL
                     DPFGIQELRELIASFPKKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            complement(18347..18859)
                     /locus_tag="PC545_000741"
     CDS             complement(18347..18859)
                     /locus_tag="PC545_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455531.1"
                     /translation="MKKISQEKISTHIQEVPQEKIEQWKSLYEKYKSILKVNSKSVLE
                     IIEFLKRKYVIVEETSEEFKHVVINNIKLNKPFSQKISSDKELKPIVFTIPNEGNAKK
                     LYDTQEEIYSGCKIIVGMEFETGYVFVEGSDELNDEITSFKGLDKYDINNYYLVANYI
                     RCLKKYKLID"
     gene            19185..19442
                     /locus_tag="PC545_000742"
     CDS             19185..19442
                     /locus_tag="PC545_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455532.1"
                     /translation="MILNRAELFRGYSLNRFNEICNILSVNKIKYKYKVKNNAYSKKP
                     FFNEITLGNLGQKEDFSYEYLIYVHKDDYEQAESLINSKLR"
     gene            19585..20211
                     /locus_tag="PC545_000743"
     CDS             19585..20211
                     /locus_tag="PC545_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HDF2455533.1"
                     /translation="MKINIKSKLSEFIKQNNLFDDSRIISYLPNITIETDKIKTIMIN
                     EVVPTNTNDDFYSVDKDADYLKTTIPLFDSAGIKVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(20458..21237)
                     /locus_tag="PC545_000744"
     CDS             complement(20458..21237)
                     /locus_tag="PC545_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901940.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2455534.1"
                     /translation="MNSKIYYFSGTGNSLVVSNILKETLNGSKEVIPLAIHRKENNID
                     INEDILVIVFPVYFADVPDIVKSFVEKLNFNINTHIYAIATCNGVAGHSLFTIDKLLK
                     KRGKKLSAGFLINMPGNALITPPDVEEERLKNQTNRVAEIAKCINNLEIGNIEGKNNL
                     KSHIDSVVLRLVGKNMQIATTKFFAENKCNRCGTCKRVCPVENISIVDKKPKWGNECE
                     RCLACFHWCPKEAINVKKSFLTNRKKYHHPDITVKDIFLDK"
     gene            complement(21305..21766)
                     /locus_tag="PC545_000745"
     CDS             complement(21305..21766)
                     /locus_tag="PC545_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2455535.1"
                     /translation="MGGARDYLNGGKMKDFEKLYEATTKLVSTSLKVKDDLKKMFKQN
                     GYDITTDHYALLRFLWEQDGISQIDLCEKSCKDKSNTTRILDVMKNKGLIVRKVDVKD
                     RRKFQIFLTDLGRELEEPLNEIASIYATETFKSISDEELPLFTDILDRIGK"
     gene            complement(21742..22419)
                     /locus_tag="PC545_000746"
     CDS             complement(21742..22419)
                     /locus_tag="PC545_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2455536.1"
                     /translation="MIITVINGSPRKNGATSKVLTYLYKDIERLIPDVKINYFDLSEV
                     NPSYCIGCLNCYKMGKCINQNDKVEYIHDIITKSDGVIFGSPTYGSSVTGLFKVFTDR
                     AHMMLERLLYRKPCIAVTTYENARGSKAISFIKSMVLDSGGYVCGSLSIKTGFNQNPI
                     TEKVESKIQKVSKKFIYCIEEKKNPPVLSQIYNFIAINAVLKPMAFKDIEQYKGIIDR
                     WEEQGII"
     gene            22652..23038
                     /locus_tag="PC545_000747"
     CDS             22652..23038
                     /locus_tag="PC545_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2455537.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            23041..24840
                     /locus_tag="PC545_000748"
     CDS             23041..24840
                     /locus_tag="PC545_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HDF2455538.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSSTNRTSMIKNSSNSVINNSNALNDF
                     SVSLNQVNFDSLNILLLISWIIGILIMIAITIHCNIKIKNMKKSIQILKDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNKDILINYIMCFLQILYWFNPLVWYAFKEMRIDREIACDISVLKKLNKDSHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNEQTVYEDLSQYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRNNSHIPWDGKHHSNSSWNEDQDLFSAMENS
                     VNWYFQTLDKKTGLKNIQTYLRQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFKFEDKNIETVKDSLLISKKDGVSLFGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGSSASKITLSILNDKKIYNP"
     gene            complement(25195..27069)
                     /locus_tag="PC545_000749"
     CDS             complement(25195..27069)
                     /locus_tag="PC545_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2455539.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
     gene            complement(27074..28738)
                     /locus_tag="PC545_000750"
     CDS             complement(27074..28738)
                     /locus_tag="PC545_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2455540.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            complement(28752..29573)
                     /locus_tag="PC545_000751"
     CDS             complement(28752..29573)
                     /locus_tag="PC545_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2455541.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            complement(29672..30436)
                     /locus_tag="PC545_000752"
     CDS             complement(29672..30436)
                     /locus_tag="PC545_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2455542.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            complement(30433..31182)
                     /locus_tag="PC545_000753"
     CDS             complement(30433..31182)
                     /locus_tag="PC545_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2455543.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            complement(31686..32291)
                     /locus_tag="PC545_000754"
     CDS             complement(31686..32291)
                     /locus_tag="PC545_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2455544.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            32470..33108
                     /locus_tag="PC545_000755"
     CDS             32470..33108
                     /locus_tag="PC545_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2455545.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            33240..33902
                     /locus_tag="PC545_000756"
     CDS             33240..33902
                     /locus_tag="PC545_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455546.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            33902..35083
                     /locus_tag="PC545_000757"
     CDS             33902..35083
                     /locus_tag="PC545_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455547.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(35264..37924)
                     /locus_tag="PC545_000758"
     CDS             complement(35264..37924)
                     /locus_tag="PC545_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455548.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(37921..38604)
                     /locus_tag="PC545_000759"
     CDS             complement(37921..38604)
                     /locus_tag="PC545_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455549.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(38948..39901)
                     /gene="blaCDD"
                     /locus_tag="PC545_000760"
     CDS             complement(38948..39901)
                     /gene="blaCDD"
                     /locus_tag="PC545_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2455550.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            complement(40442..42556)
                     /locus_tag="PC545_000761"
     CDS             complement(40442..42556)
                     /locus_tag="PC545_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HDF2455551.1"
                     /translation="MSENKQVVNCDIIASNSKHNMCEYDAELLTFFPDEIAHLIEINN
                     KLDNAFKKAENLVDKLDKDYMDAKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKINKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKADTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     SERVRFKSTLDFVKLIDDYIKQMPNKIFIPKDYTFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKVKSILKSLNGMLTIKNTLTLYKDFFKQMNISN
                     MFVMAEKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHIVIDEMQDYTPIQYAVINLLFK
                     CKKTILGDFGQLVNPNHTHTLDDMKQLYNDGELVMLNKSYRSTFEIINFAKKIQDISS
                     LEPIERHGEEPALLKCNNEQDEINKIKIEIEEFKKSDNATLGIILKTDNDAKAVYDAL
                     REEYSVHLISSESSSFTKGVSITSIKMSKGLEFDEVIVPSVNNKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTCELTQLIDM"
     gene            complement(42640..43476)
                     /locus_tag="PC545_000762"
     CDS             complement(42640..43476)
                     /locus_tag="PC545_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455552.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLASMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSDIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     LS"
     gene            complement(44060..44422)
                     /locus_tag="PC545_000763"
     CDS             complement(44060..44422)
                     /locus_tag="PC545_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2455553.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDDALKPFIETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQD"
     gene            complement(44656..45120)
                     /locus_tag="PC545_000764"
     CDS             complement(44656..45120)
                     /locus_tag="PC545_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2455554.1"
                     /translation="MTRISNITIAEQSEYCFLAIRKTIDFMVEFSEFSKQSFEKISKY
                     LEERGILSSGAPIVCFHNMDLVKLDVEVGFPVATYIDGKNEILQKIVPAQKIITAIDL
                     GLYELQDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
     gene            complement(45549..46970)
                     /gene="nhaC"
                     /locus_tag="PC545_000765"
     CDS             complement(45549..46970)
                     /gene="nhaC"
                     /locus_tag="PC545_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2455555.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNIFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPIGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLRLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDV"
     gene            complement(47000..48064)
                     /locus_tag="PC545_000766"
     CDS             complement(47000..48064)
                     /locus_tag="PC545_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HDF2455556.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEIV
                     EEICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFNNVREIVRLKNIEIIGIATNLT
                     CYGAIIPSRENIGRLVSIAKRMEEKFGINLEIVSGGNSSSIHLLINNNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGVDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDNLEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(48049..49458)
                     /locus_tag="PC545_000767"
     CDS             complement(48049..49458)
                     /locus_tag="PC545_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HDF2455557.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHDINTNNPIFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDDIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQITSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNVENQEEIRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DRSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKVKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(49458..51662)
                     /locus_tag="PC545_000768"
     CDS             complement(49458..51662)
                     /locus_tag="PC545_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2455558.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYK
                     DCTKPLKKSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMVRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLKIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELRDKARELKERAVLYMEEISEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDENAIDNPSMLIDGCTFENPDKIIYIDELDDFDNVENRLKESYEYRNGTKIKPEME
                     WCADGIIMITMMLPTDKRTAEFAALEFVKKMNLQEIEVISREVMHESEGTRIEVKGRV
                     PFDIDLNNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMSNK"
     gene            complement(51656..52030)
                     /locus_tag="PC545_000769"
     CDS             complement(51656..52030)
                     /locus_tag="PC545_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HDF2455559.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVKGEYWDEVTCLFREGVESC"
     gene            complement(52108..53538)
                     /locus_tag="PC545_000770"
     CDS             complement(52108..53538)
                     /locus_tag="PC545_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HDF2455560.1"
                     /translation="MNNSTSMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEAIQSQYAVGNTPLIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKQMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKAIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVVDVLNNL"
     gene            complement(53531..53839)
                     /locus_tag="PC545_000771"
     CDS             complement(53531..53839)
                     /locus_tag="PC545_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HDF2455561.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKINPYYTHDYGKCIPELLQIGIQAKEILFGGVYNE
                     "
     gene            complement(53960..55027)
                     /locus_tag="PC545_000772"
     CDS             complement(53960..55027)
                     /locus_tag="PC545_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HDF2455562.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKINFLLNRKINVIS
                     TAEQMAYPQADDADLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGVTREEFIKGVEDKTIAGHVGFVESINMIADGLGWKL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(55283..56656)
                     /locus_tag="PC545_000773"
     CDS             complement(55283..56656)
                     /locus_tag="PC545_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2455563.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREVIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSIRQKKGYEFDDILGESNLIKEAIAKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     KVVGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYTLKEN
                     KESNNYSLNDLEDKTLSKIISEIEEKYIYESLEKASYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT        19244 a         9668 c         6751 g        21121 t
ORIGIN      
        1 tgaaaaagca cctcattctg attaaaaatc tttctgagat actttttaaa atattaaata
       61 ctacatatag cttacataag tttaaaattt aatctattat aaaattaccc taattgccaa
      121 actttttaat ataaattttc caacatccta caaatataat caatagatat agaatcctaa
      181 cttacaccaa tatatattta tttaaaatag tcctaaaata cctgtaatag ctcccactac
      241 tatcagtata aacattattt ttattggaga aagacctttt tttaaagcaa agagtactaa
      301 taaagtaagt accaaaggaa ttaacccagg aaatatctta tcgagtaaat ccgtttgaag
      361 actaaattta aaatcaccac tagtataact tattggtgta gataaattaa taaacctacc
      421 tacaagtccc cccatgacta aggtacctag tatagaagcc ccttctataa ccttatttac
      481 tattccacct tgaagtatac tttcaacggc ttttttccca tacttatatc catttgtcca
      541 cattccaaaa gaaattgaag tcattattat atattgagct attacaaata atataggtcc
      601 tgcaattgat gctcctccct cagttaaacc aatacaaaca gccaacacta taggagttat
      661 aactccttga gttaaagtat cgccaattcc cgctagtgga cccatcaagc ctgttttgat
      721 agcattcata ccatctccac ttatttcatc tgctccatta gctttttctt cttccatagc
      781 tattgttatt ccatgtataa cacatccaca tataggttct gtgttaaaaa atgctagatg
      841 agtctccact tcttgtttta actcttcctt atctggatat aattttttaa gtactggcaa
      901 cattgaatgt gaaaaagctg ttgattgcaa tctttcataa ttgtaattag attgtgcaaa
      961 gaaaaaccat cttaaccatg attttattac atctttcttg cttaacgttt tcttagatac
     1021 ttcatttttt atatctgaca taatattttc cccctcacat gtatttaaga tgtagcacta
     1081 tcttttccgt acttaattga agtaattgta agagcaagta ttactccaat aacagatatt
     1141 actattatat ctactttaaa ataaacagcc attagtattc ctaatatgaa aaagggcata
     1201 gtaaacttgt ttccaatcaa ttttaaattc atagcaatcc caagtgctgg caacatagcc
     1261 ccaattacat taaatacatg taacacatta tcattcatta cttccaacat accatctatc
     1321 gcatttggac caaaataaac aactaataat gctggtataa aactcattgc aaataacagt
     1381 atctgtggtg gtactatatt tataaatgct actctatcaa cattcccttt ttgaacctct
     1441 ctatcagccc aatgtgcaaa aaaagaattt actgtcatct ttccaatcca aagtacagtt
     1501 gcaacagttc ccaatggtac agctattgcc attgcagtac ttacatctag cttagatgct
     1561 atagctaaag cagtacctac atatcctgca aaagctgggt cccccatctg agctccccct
     1621 gctgatataa aacctagata tatcatattt atacctgctc ctataattgc tccttgaaca
     1681 gggtctccta atataattcc cgttacaaat ccagccaata gaggtctatt ccatgtaaaa
     1741 aatccaacat aagtcaacca cggactccaa gaaagataat aaaaaattgc tattaatact
     1801 gcttgaatta gtgaaatgtg catattcttc ctccctattt tcttttaaag atttacaaga
     1861 tagtcttctt tacaataatg ttcctacatc aattttcttt gcatctggta ctatttgtat
     1921 aaagcattta acatcttttg atattagctc tctaaatatt tctttttctt catctgacac
     1981 agatatattt ttataaaaac tttttctacc aagccctgct cccatacctc caacattcaa
     2041 ttcctttaaa tctaccccgc tatttactaa acctaagaca gttttagggt atttgaccaa
     2101 tactattaaa ttttcatctc cattatatga ttttaagact tcagcacctg attctacatc
     2161 atgtacttca accttaacag ttgatggagc tgccatttgt aaaacttgaa ctataaatgg
     2221 gtcctttact actaaatcat ccacgattac tatccttttt gctgatgtaa tcttacacca
     2281 agcagtaata acttgcccat gtattaatct atcatcaatt cttgttaatg ctatattact
     2341 cataattatt cctccagatt tttaatttat tttacaactt taaaacagtt ttaacatctt
     2401 tgattcctga tatcccaact tcctcacata aattcttcaa gtcttttaac ttcatagtat
     2461 ctctgtttaa aaataactct aaaagcatag gtaaatttac acctgtcaaa cattctaact
     2521 cacatacctt agcaaattta tttattataa tagcagatgt attaaatgga gttccgccat
     2581 aaaaatcaag caatactaaa accccttcat ccttatattt ctcaattcca tcttctaact
     2641 ttctactaaa ctcatctatg ctgtcaccat ggtttaatcc taaagtttct acattttcct
     2701 gtttgcctac tattaattca gcactttcta acattgcttt agccatattt ccatgtgtgg
     2761 ctataattat tcctgccata tgaatcatac ctttcttaac cttttttata tggattatta
     2821 tttagtactc taatctaatt atctatatta atcattatct taccaaagaa aatatccctt
     2881 ttgtatactt tttcaaaata agctggtact tcttctaaat ttattctatg tgttactaat
     2941 ttttcaacat tcatgtcacc cttttgcata tgatacaatc cagcactcca ttcttttcct
     3001 gggaatggaa aggagtttcc aaaccaagtt ccctttacag ttaattcact tcttactatc
     3061 ttttcaaatt gctctctagt caaagctaca tccccatatg gtacacctgc atacaatact
     3121 gtacctccct ttttagctag caacagtact tgaccacatg tcagtggagt accagccgat
     3181 tcaataacaa tatctgcacc atctccatca gttaaccttt ttatttcttc tacaatattc
     3241 ttttcttttg catttataca aatatctgcc cctaactctt ttgccaagtc taacttctca
     3301 tcaaatacat ccacagctat tatttttgta gaaccaaata tttttgccca ttgtattgaa
     3361 aataatccta ttggacctgt accaagtact acaacagtat ctccaacttt agcttctgac
     3421 ctaaaaagac catgtcctgc tatacatact ggttctagcg cagcggcagt ctcataactt
     3481 atctcatctg gtatttttat taaatttctc tctttcacct ttgtatactc tgcaaaacaa
     3541 cctccaagtt ctttatttcc tattattgct acattattac atcttgaata caagcctttt
     3601 ttacattcat cacattcaaa acatggcata gctggacaaa cagcaactct atctcctatt
     3661 ttaaaactcc ttacttcttt accaacctgt gccacttctc cagaaaattc atgccctaaa
     3721 atttctccaa ccatatgtgg acctgtcttt gaatattttg atatatcaga accacatatt
     3781 ccagctactt ttactttaat tattacatca tctttttcta atatttttgg tacatccaca
     3841 tcctccactc ttgtgtctct tataccatag aatctaactg acttcactat taaccacccc
     3901 tactttttat ttgagaatat atccctattg tttatatgtt aaatatctaa agcaactttc
     3961 atgccaatat atgtaagcat tgtattcatg cattttataa aattatcatt aaatgtgtgt
     4021 caaagtgtgt caaaagtttc ttatattaaa acttatgtgt gtcaatataa acgacactaa
     4081 cattttatat tagttctctt attataagtt ttataaaatg tacttttttt gttattttta
     4141 taaaattgtt cggaaatttc ccttctattt gtgatataat attaaatata aggtatcaat
     4201 ataaattttc atgcgaatat cagaactttt ttgacactaa tgccttattc aatataataa
     4261 tttaactttt tggaggttgt ttatgaaaaa agtgtatcaa ggaaagacta aagatgtttt
     4321 tgaattagat aatggaaatt acttacttaa atttaaagac gatgtaactg gtaaagatgg
     4381 agtatttgac ccaggcgaaa atagtgtagg tctaagtata gatggaattg gtagagctaa
     4441 tttagagaca tctgttaagt tctttgaaat tctaaacaat gctggaatta aaactcatta
     4501 tgtaagtgct aatttagaag aagcaactat ggaagttcta ccagcaaaag tttttggtaa
     4561 tggattagaa gttatttgtc gttatagagc tgttggtagt ttcttacgtc gttatggttc
     4621 atgtgttgaa gaaggtgcta aactagattg ttacgtagaa gcaactttaa aagatgatga
     4681 ccgttgtgac cctcttataa catctgaagg tttagaagct ttaaacatta tgactcaagc
     4741 tcaatttgat tcaatgaaaa gcatgactca aaaaatttct aacatagttc gcgatactat
     4801 agctgaaaaa ggtcttgagc tttatgatat taaatttgaa tttggattct acaatgatga
     4861 agtaatctta atagatgaaa tagcatctgg aaatatgcgt gtttataaag atggtgtaat
     4921 agttgaccct atggatttaa ctgctctttt attagataaa tagtatatac atataattta
     4981 caaatataat aaacttataa ataagataag ctatgaagta aagacttttt aaatgttgat
     5041 tatttttaaa cattttctcg cctttaattt catagcttat ttaaattaat atactctaat
     5101 tagtagataa tagctttaac cctacaattc ccaccacaat aaaacctata catgcaattc
     5161 ttgatatact aataggctca tttaaaatag ccatgcctac aatagctgta ccgacggtac
     5221 caattccagt ccatattgca tatgaagttc caagtggaag atgttttagt gctaatgaca
     5281 aaaaataaaa acttacaatc attccaacaa cagtaagtat acttggagtt aatttagaaa
     5341 atccatgtga attttttagt tgaaaagccc aaaacacttc aaacaaacct gcaacaaaca
     5401 aaaataacca tttcataaat tcatccctct tttcaaatat gtctcttatt tttccaataa
     5461 aataacctga gaaatattat aaaatatctc ccaggttttt gtccttccgt gttcacaaca
     5521 aaaactatgt tgtgggtttt atctcggacc agaccaatca ttaataattg cggaacccta
     5581 taaaactcta ttaattattc tattttatct ataataatac tatcaacata tataaaaaaa
     5641 gtcaatatat gggttccata tataataaaa ataactttta tatacctttt aatattctta
     5701 ttcacttctc tccataccca tttgggtcaa tctaagaata tctaattttt atttaatatg
     5761 ttataataac ataaaaatat tgaaaggagt aatttctatg aatttctata aaattgcagt
     5821 atgtcagatg attactacag aaaataaaat tgaaaatatc aatcatgctg tagatatggt
     5881 aacagaagct gccatcaatg gatctaaaat tgtagttttg ccagaaatgt ttaactgtcc
     5941 atatgaaaat aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa caacaacaat
     6001 attgagtaaa ttagctgaga aacatggaat ttatctagtt tctggtagta ttcctgagtt
     6061 agaagatgga aaaatctata atacttgcta tgtatttgat aaaaatggag ctctaattgg
     6121 aaaacatcgt aaaatgcact tatttgacat tgaagttaca ggaaaagtta gttttaaaga
     6181 atcagatact cttacagctg gaaatgatgt tactgtaata gataccgaat atggaaaagt
     6241 tggtatagct atttgttatg atattcgttt ccctgagtta tcacgtttaa tggctcttaa
     6301 aggtgctgaa atcgttatct tgcctgctgc atttaatatg actactggtc ccgcccattg
     6361 ggagttatct ataagaatgc gtgctttaga taatcagata ttctatgttg gtgctgcacc
     6421 tgctagaaat atgaatgctt cctacatagc ttttggaaat tcaagaattt cagacccttg
     6481 gggaagaata attgctcaag ctgatgaaaa agaatgtatt atttatgctg acattgatag
     6541 agatttaatt cctgacataa gacaacagtt accacttctt aagcacagaa gaactgattt
     6601 atatgaactt aatactttga aataaaacag aacataaccc cataaatcat aaattaagtg
     6661 ttaaaaaaat ttatggggtt ctcattaatt tgatttaaaa ttaatttcat aaaaataata
     6721 ctaatattaa tttaattaaa taatactaat aattactaat gattatgcta attatcagca
     6781 tttactaatt taatcactat tagttatttc ttttattaaa tatctttact ttttttactt
     6841 attatgtctt ctagctttac atttggatta tagtatttct ttctattttt agtcacatct
     6901 ttctattgaa ttacttgctt aaaacaccaa tgcacacatt ccagacaatg ttcacaatta
     6961 tgtaaaaatc ttgctctacc ttcttcaatg ttgatattat ctttcctaaa atttctttta
     7021 attaaaaaac aacattcgtt gatatatcga gtatatttta agcaactgaa aaaaacaata
     7081 ataattgata tatcaactaa ttaatgtaga ttaattcatt tttataattt atatactttt
     7141 taattaatct acctttttaa ttaaaattct tcctttctat ttatccattt taaacttaaa
     7201 ttataagatt tcatcagctg tgtttttgca ttccaataag tattaaatat caaatatata
     7261 ttctattaaa atttctaata atttcatcct tgatttattt aatatataaa aatatactaa
     7321 ctttaatgat taagttttga aatataacta agaatttgag attttattga gatttttata
     7381 ctaaagtata catactatac aagcttaatt tagaagggag cattcttatg aacaataaaa
     7441 tactcgtcgt agatgatgag gaaggtataa taacactctt aaaagattac tttgaaatga
     7501 atggttatga ggtttacact gctcaaagtg gaaaaggcgc attaaaaaaa atatccaaaa
     7561 atccagatat tattctactt gatataaaca tgcctgaaat agatggaatt gaagtttgta
     7621 atagaattag agattatgta tcatgcccta ttctatttct tacagctaga attgaaaata
     7681 ctgacaagat aaatggtttt cgtgctggtg ctgatgatta tattgtaaaa ccttttgatt
     7741 tagacgaact tggagctaga gtatctgctc atttaagacg tgaaaaaagg aagtcaaatc
     7801 aaactaaaat taaattttat aatgatttaa cgatagatta cgaaaaaagg ttaatttgta
     7861 tagataataa tcagatttca ctttcaaaaa aggaatttga tatagtagaa cttctctcta
     7921 tgaatcctgg acaagtattt gataaagaaa aaatctatga tagagtctgg ggatacaata
     7981 gcaatggaaa tagtgatgtt ataatggaac acattagaaa gatacgaatc aaactatcaa
     8041 aacatactct ggaaaattat atagaaacag tctggggagt tggttataaa tggataaaat
     8101 aaataatatg tcattaaaaa aagctttctt tgttcttact ttatcttcac taattatagc
     8161 atcaacactt actactttct catacatttt gcttaataat ctatataatt ctatacaaga
     8221 taagtattta aaaacaccta taaatacggg ttttataacc atagttcaaa acaatgtgaa
     8281 ccaatttagt gattatgata aaacgttact taatataatc aacatacttc agcttgtact
     8341 tccacttata ttttttatag gacttttatt attagctgat ataatttttt acaaggtaaa
     8401 attaaaaaca cccatagaaa tactcaataa aggtgcaatg gaaatatcca acaacaattt
     8461 agacttttgc ttagagtata ataataatga tgaattaggt aatctatgta atgcttttga
     8521 aaaaatgagg tctgaactaa ataaaaacaa cataaagatg tggactatga ttaatgatag
     8581 aaaacaactt aatgctgctt tttctcatga tttaagaaat cccctaacag ttctaaaagg
     8641 ttactcaaac tatttaacaa aatacatacc aacaggtaag cttagtgatg agaagatttt
     8701 atcaaccaca caacttatgt ctgagcatat taacagaatt gaatactatg tagaaaatat
     8761 gagtaatgct caacgattag aagatttagt tgtttcaaaa tctatgtcga atataaacaa
     8821 gtttatagaa aacttagatg aaaatatatc tatcattgct aaacaagaag gaaaatcttt
     8881 tacacttaaa agtcaaataa ataatataaa tctatttttt gatgaaaata tcattcttag
     8941 agtcgtcgaa aatataatat cgaatgcttt tagatatgct agaaataatg tatccatact
     9001 tatatatctt gaacaagaac tccttacctt tgtaattgaa gatgatggta taggatttag
     9061 taaggaatct ctaaaattag ctttaagacc tttctataga gataagactt taaatgacag
     9121 caatgcacac tttggtatgg gtctttatat ctcaaaaata ttatgtgaaa aacatggtgg
     9181 ctctatttct attgaaaata attctactaa cagtgctaaa attactgcta aattctcaac
     9241 aagaaattaa aatgattttg taagctttca ctgctattat ataaaatttg ttagtttaga
     9301 atttataaaa tgtatttcaa tttataacca atatatatac tattttgtag aaattgtaag
     9361 agttgcttca attataatat tttggcaaaa ggcatttgaa acaataatag aaatatatat
     9421 ttaaatatgt agaatagtat gaaattagaa ttttggaggt gattatatta aaagttttgc
     9481 taataatatt acattctctc gtattgtgtt tgcattgcta atgcttagtt ttacattctg
     9541 ttcactgcct ttctggatat gttatttttg gtgtggaatt agtgatatat ttgatggtct
     9601 tattgcccga aagttgagac aacaaagtgg aataggtgca aagttagata gtattgctga
     9661 tttcattttt tcggttacaa ttttggttac tgtcggaaaa aatattgctc ttcccatgta
     9721 tctatggtgg tgtattgttg caatagcatt gttacggttg attagttaca gtattggttt
     9781 ttttaagtat cgcacatttt catcgcttca tactttgatg aataaaatca ctggaatttc
     9841 tatctttgcg tttccattac tctatatttg ctttggtctt gatgttgcag gaattatgat
     9901 atgtatcgtg gcttttcttt cttcttttga agaaatagca attatgataa aatcaaaaga
     9961 attaaatcgt gatataaaaa gtatatttat gtgaaaactt agaatttgtg acaaaaataa
    10021 tttatataat aatgaggaag taaaacataa aaaattatcc acaagtttat gtgtaaaata
    10081 gttttaaagc aatagagttg tattgtaaag cctttagtgc ataaattaca ttttaaatta
    10141 aaaatgatac tgaaactgct tatcagcact gcgaattatc cgcaaatagt gaagactttt
    10201 tagatttaag ctaaaagttg ggaaaaaatt gagttttaaa acttacactc ttcttatata
    10261 gaaaaggaga gtgtttttta tgattataaa agcaaaacaa ttatcaaaaa tatatggttc
    10321 taataacaat aaagttatcg cacttaataa tgtaaattta gaaataaact caggtgaatt
    10381 tgtttctgta attggacctt ctggaagtgg aaaaagcaca cttttacata ttttaagtgg
    10441 attagataat cctacctctg gacaagtgtt acttgatgat aaagatatat ataaacacac
    10501 tgaaaaagaa ctatctgcac ttagaagaaa atcctttgga ttcgtctttc aacaatttaa
    10561 tcttttacct gttctgacag catctgaaaa tatttcaatg cctgtacttc tagataaaaa
    10621 acaacctgat aaagaatatt taaatgaaat ctcatcatta cttggaattg cagatagact
    10681 caatcatctt ccccatgaac tttcaggtgg tcaacaacaa agagttgcaa ttgctagagc
    10741 acttattgca aaaccagata taatatttgc tgatgagcct acaggaaact tggacagtaa
    10801 aagtggcagt gaagttatga atcttcttat aaaaacttca aaacagtttg gaaagacttt
    10861 agtggtaatt actcatgatg atagaattgc aaaacttgct gatagaaaaa tttcaattat
    10921 tgatggtgtt cttatggagg tgaaatagaa atgaagaatt acctttcact ttcaataaaa
    10981 gaattaaaaa gccagaaact tatgactaca ttcatcatta tagcaatagt actatcaact
    11041 attatgacaa ctgtggttgg acagtcaatt ggtatcctac aaaatttaag aattgaacaa
    11101 gcccgttctt ttaacggtga tagacatgta tcctttcacc aacttactaa aaatcaagtt
    11161 gatgacataa agaaagatga tagagtttat caagctggta cttcaataac tcttggcagt
    11221 agtaaaataa aggattctgg tatctctgta cttgtaaaag agtatgataa aacaggactt
    11281 tccaactacc ccaaattaat gaaattaaaa tctggaaatt tgcctaaaga taaaaacgaa
    11341 atagccttag atgaaaacac actaaagctt atgggtataa aaccaagact aggagtgact
    11401 atacctatga atttagatat ttcactttta aatgatacaa taccaccata taattacaca
    11461 gctaacttta aattgagtgg tattttagag gatgattata caggatatgt aagtggtata
    11521 gtaaacggta ttgtaggcaa aggtgcatca gaaaatctat tacctaagag atatatttta
    11581 tcctctttgg attttaaaat aaaacagcaa gagaagtttc aagaaatagt aaatcaatta
    11641 gctaaaaaaa taaatctttc tcacaattca attcaatata attggattta cttaaatgct
    11701 cttaaaatac agtttgaaaa agatgaaaat agttctaata gtgatggtat atctatgatt
    11761 atattggtat ctttatttgt tgccttattg gttttactgg cttctggact tgtaatctac
    11821 aatattttaa aaatctctgt tacaaagaaa attaaggagt atggatgttt aagagcaata
    11881 ggagctgaac caaaccagat atataagata gttattttac aaatcttaat tttatgtact
    11941 attgccatac ctattggagc tgtaattgga attatatctt ctaaaggcat aactggaatg
    12001 gtcactaata tcttaaatcc agatatactt ctagcaaatg ataataaaga aattactgag
    12061 cttatacata aaaacacaac agcatatata ttcccattag tattaagcac tagtatatct
    12121 ttgatttttt cacttatttc agcattacca tcagcaatat atgcttcaca tgtctctccc
    12181 aaaattgcta tggctggcag tactaccaaa atcaaaagaa aaattaaacg agaaaaaaca
    12241 ataaaaaact ttgaaagaca tctagcctgg ctaaacttaa aaagaaataa aggaagaact
    12301 ataataacca tactatcatt atttatgagt ataacagtat tcgttgcact gagtgagttc
    12361 tctaatgtat tagatgtatc taggtcagta agtaatttaa aagaaggaga tttttcctta
    12421 acaaatgaaa tatctggttt tgataagtca atactagata aaatagataa aatgaaaaac
    12481 gtcaatcgca cttcctttat aaagtactct gaatacaaac agggcgaaat agatacagat
    12541 ataaattttg aaaatagtgg tgagatgtta aaaatcattg gaattgatga gcaaacttta
    12601 aaagacttaa tgccttctat tacagattct atactagaag actttaaaaa tggttctatc
    12661 tgttttataa aaaatccgat tgctatttct acaccaggtg taaaaactag acatacaaat
    12721 ttaaaaccta aagataatat cacaattaac aaaaaacaac tagatatata tagtacagta
    12781 gataaaatgt tctttttaca aggtaatggc tggataaatg gtgttgatgt tattgtatat
    12841 gattctgttt ataacacctt gacaaataaa aacaagttca accagataaa tatttatgct
    12901 aaagataaat caaaattaga acaaataaga ttgtctattg aacagatttg tgaaaataat
    12961 cctggctctc attggatatc atatattgaa tcagataaac aacttaaaga gagttttaaa
    13021 caaatagaat ttctagcatg ggcagttata ttatttgtgg gattgattgg tactttaaac
    13081 atcattaaca caactcatac aaatataaat actaggacta atgagattgg agttaaaaga
    13141 gctattggta tgagtaatag tagcttatat aagatgtttt tatgggaagg tgtttattat
    13201 ggaatttttg ctactatatt tggttcaatt gctgggtatg tcagtgctat tattataaac
    13261 atggcaacta tagaaaagct tgattttact aatataccaa ttacttcaat tttacaagct
    13321 actattattt ctatacttgc atgtattata gctactctta ttccgttaag aaaagttaaa
    13381 aagatgaata taattgactg tatagatgtc aatttataac tattttacag ataataaata
    13441 aattaaatta tcaatgactg tctctatttt cttttaaatc taactaataa atctttttaa
    13501 ctataatata agctttaaaa aaggcctgtc tcaaaataga gataagcctt ttttcattgt
    13561 cgaaataatg aaaatatatt taacttcaca aaaaaagagt gcctcacgaa ttaaaaattt
    13621 cgctttgaga cactcccctt taatttaaaa tatatagtta aatgctatgc caaaatttca
    13681 cacatgctcc acctgatttt gaattttcta ttttaacaga acctccaagc tgttccacta
    13741 attgttttac aatgtaaagc ccaagaccag aatgtcctcc tttagtctgc ctagcttcat
    13801 ctcctcgata aaacttatca agagcctttt ttaaatcttt agagctaaac cctctaccag
    13861 aatcacaaat ctcataagaa atacaatttt tttcatcctt tacagtaata ctaatattac
    13921 cacctgatgg agtgtattgc agactgttgg atatgatatt atcaaaaatt cgtgtcaacc
    13981 tatccacgtc gatttgaatt ggggattgta tattcccctg cattttcaat ataagttcaa
    14041 cttccttttg atgagcctgt aattcatagt cttgtacctt ttgcctcaaa aattcaggta
    14101 aatttatagg aacaagattt aattgtatat ttgacttttc taagtcagac gtatattgca
    14161 tcttttggac aagagcagca cttttttctg tattttctct tatcactgta agatacctat
    14221 gaagtttttc attgtcatct gtattattac caataagtgc atctgtatat cccaaaataa
    14281 tagaaagagg agattttaaa tcatgtgcta gagcttccac catttcaaca cgttcctgct
    14341 ccatcttcca ctgtgcagat aaagagcctt ttaattcgtc tttcatttca gaaaatgcac
    14401 tacaaagttt accgagttca ttatctgaat aatagtcgat ttcaaaatca aggtcctttt
    14461 cttttatctt cttggacgca tcaatcaata gatgtaatgg ttgattgata ttctttacaa
    14521 agcgcttaga aagccatcta gtaaaaccca cgatatatag aaatggagaa aacagagcca
    14581 ttataataac tgcaaataca aatctccctc taatatttgc aaaggtgatt ttcacttggt
    14641 agaatagaat aacagctccc tcaatctttc cattatcccc tttgattgga acagtatgga
    14701 tataaccacc tttacgaaca gttttattga taaaattatt aaataattct tcttttgttt
    14761 taaatagttt tttggggtta gtaccatata aaatattgcc attattatcc acaacttgat
    14821 atagcatatc atccccacga atcgttcttt ttaaaccttc ttcattcgat tgtgataaca
    14881 aagttatgtt tttttcattg atgtattttt caattctagg aacttgtcgc tcataatagt
    14941 ttggtggata gatatcctta tttaaggaat gaatatacaa atagagagca aatacataag
    15001 taatcactgt ggcaactata ctggctatta taataaaaat aaaagtatgt ctaaattggg
    15061 taataaggga ttgatttttc atttaataac ctctctctct ttacatttta ttccacttat
    15121 acccaatacc ccaaactgtg gaaatatatt ctttttctgg tgtcacagca gaaaacttag
    15181 ctcgtatttt ttttactcgt tcaacaacag ttgaggaatc accttcagaa tcacaccccc
    15241 atactttttc atagatttgt tctcttgaga aaacttgtcc tgcatggagt gctagtaatt
    15301 caacaatatc atactctatt tttgttaaat caatattctt accatcaatt ttaattgcac
    15361 gttccttcat atataagcat aacttcccaa aatacaaagt agatctcttg ttctcttcat
    15421 tgatatactg tgaacgcttt tccctacgca gattagcttc aatccttgcc ataagttcac
    15481 gcagtccaaa tggctttgtt acataatcat cccctccaaa ggtcaaccct ttgattttgt
    15541 cggtttctga ttgctttgca cttaaaaata taattgggca taaaacatca tctctaattg
    15601 cctgacatac ctcaaatcca ttcattttag gcatcataat atccagtata ataaggtcag
    15661 gctcctgttt tgataactct atacctgcct gaccatcata agctatcaac acttcatgtc
    15721 cttttgcctt caattcatcc tcaagtagca ttactaggtc catttcatca tcaataatca
    15781 atattttact caatataaca ccacctttct cttatgggaa ggtcagtatt aaaacaatat
    15841 tcgtctacta ataacttttc cttccctccc acattttaaa ccaaataacg cctcctatca
    15901 agaatataat caaacctatt gctactacta taagctctat actagctgtt tgcatcactt
    15961 tagagtaaaa tgcctcagtt gaaatagttc cagcttcttt aataaaataa attcctggaa
    16021 gcatacctag ttttactggc catgtccatg gaacaaatgc ccatactttt tcaccaacgc
    16081 tagttaatcc aagtattgct gccatcaaaa ttccaccgat actaatacca atagatgcac
    16141 ccatacccca tgcaaaactc acccaaagat gaatagctaa tagtggaagt gtcccaatca
    16201 ccacaagtcc tgctgctgac aggaataacc atattgttgc tccagatggc actgcaatac
    16261 tcattcctat gcaaagtatc aacgtagcaa taaatgtgca tatagttagg caaaacagaa
    16321 gaaaaagaaa ttttcccaga tataatctaa cacgagaaat acctacacat aaaaaaccat
    16381 taaaatttcc agcaagctct tcctcctgca ctataaatcc tgctaaaata cctactccaa
    16441 taggaatgat gaatacagtc catactgtaa aaaatccttc aaaagcaaat tcttgtgtac
    16501 ttccagattt agttgtgaga taaaaaacca cacataatga aaaaataata ggcataaaaa
    16561 gagtaagcca ctggacagct gtcctctttg ctcgaagcca ttctgatgat aatagcttta
    16621 tcatttgtca tccctcctat tgaaccaagc tgcagtaagt gctgttaata ctataaacac
    16681 aaccaaagaa acaacaatcc caataggaat cactgaagta ttcaaaagtg ggtctccagc
    16741 ttctaatata gtaccgttag gatgtatgtt gatgattggg cataccattc taactgccca
    16801 actccaaggt actgcaatcc aaaatggttt tgtcgcagca aggacaccaa atatcatacc
    16861 taaagctcca actcccatac ttaggaacat acctttccat gtagctagcc atagctgaag
    16921 tggaagtata gcaagagaaa caacccagca tacaatactt gcccctataa tctgtccaaa
    16981 aggaactgga ccagctttca atattaaacc agttataatt gttacgacta ttaaaactag
    17041 cgttgaaata aaactgtaga ctgccatagc tataacttta ttaatccaaa gtgtcatagg
    17101 agacacattg tgtgtacgta aagcacgata atttccagct tttttttcct ggaaagctac
    17161 caaagtagca aacaatgcaa atccaatagg cagaaataaa aatgaccacc agttaaaaat
    17221 taatgctaaa agactttgcc acgcccaaga agttgtttga gataatggat tttgcatgag
    17281 tgttgattgc attataaatg catatgctgc aaaagacact gggaaaaaaa caataagttt
    17341 acccataaat gttcttttat actttaaaag ttctgattgt aaagcattca tctgttttgt
    17401 cctcctttaa gaataacatc tgtaaaaaat tcttctagat tttcattagg gtcaggcatt
    17461 ccttgaaata atagtcgtcc tccatttata attcctatat tatctacaac ttgagcaacc
    17521 tctgatagaa tatggctaga aagaattaca gttatacctt tttttggaaa agaagcaatc
    17581 aattcacgca attcttgaat gccaaagggg tcaagaccat tggttggttc atctagtatt
    17641 aacaactctg ggtcactcaa aagtgcaata gctattccta acctttgttt catacccata
    17701 gaaaattgtg aagctctctt tttacctgta tttttcaaat ttactatttc caacacttca
    17761 taaattttat ctttaggtat tcccaatatt tttgtatgta ctaacaaatt ttcctctgct
    17821 gtaaggtttc catacaatgc aggggattct attaaagaac ctatttttgc aagagattct
    17881 cgtttccaag gctttctatc aaaaataata cgaccatttg ttgggcgaag aagaccaaca
    17941 agcattttaa gtgttgtgga ctttcctgct ccatttggtc ctaaaagtcc atatatacta
    18001 tttcgttcaa tttgtagaga aacattgtta actgccattt gttctccata tctttttgat
    18061 aagttttttg tttcaagaat taaatctttc atattaattt ctcccttctt gtaatacctt
    18121 aaatctgtta ctaagagaat actaacatga aaaaataaca attctataac acctactaat
    18181 tttaataaaa tttaaaataa aaaatagagt taccaatttt attttggcaa ctctatttaa
    18241 gtttatgttt tacagttata ttagatttaa gcttagtttc attatcacac taacttcaaa
    18301 tttactctat aatccttaaa tcaaacacta atttattaat caaacactaa tctattaatt
    18361 tatatttctt tagacatcta atatagtttg ctactaaata ataattattt atatcatatt
    18421 tatctaatcc tttaaaagaa gttatttcat cattaagctc atcactacct tctacaaaaa
    18481 cataacctgt ttcaaattcc atacctacaa taatcttaca gccactataa atttcctctt
    18541 gagtatcgta caattttttt gcatttcctt catttggaat tgtaaataca ataggtttta
    18601 attctttatc actggaaatc ttttgtgaaa aaggcttgtt taacttaata ttattgataa
    18661 ctacatgttt aaattcctct gaagtttctt ctactattac atattttcgc tttaaaaact
    18721 ctattatttc taaaacagac ttagaattta cttttaaaat acttttatat ttttcatata
    18781 aagatttcca ctgttcaatt ttttcctgtg gtacttcttg gatatgtgta cttatttttt
    18841 cttgagatat ttttttcaat taaactctcc tttcaatact taaatatcct tataaatctc
    18901 acatgactaa agttacaagg ttcctactta atagaatttt gcatacttaa atatgtctta
    18961 ttaattctct acaggatatc tccgtagttc ctacgattct tacataagtt acttatttta
    19021 aattgttatt agttagatta taaaaattat ttattttatc actaatataa tactcttaaa
    19081 aactttacaa atcaatttaa atttgctacc ttttatttat aacaacttat agtacatttc
    19141 tcctatgctt ttcaatatat tacttttaaa aggaggtttt cattatgatt ttaaatagag
    19201 ctgagctttt ccgaggatat tctttaaata gatttaacga gatatgcaat atattatcag
    19261 taaataaaat taaatataag tataaagtga aaaataatgc ttactctaaa aaaccttttt
    19321 ttaatgaaat aacacttggc aatttaggcc aaaaggaaga tttctcttat gagtacttaa
    19381 tttatgttca taaagacgat tatgaacagg cagagtctct aataaattct aagttgcgat
    19441 agattatcta taaaacatga cattctgttg tcatgtttta tatgttatta tactttcaaa
    19501 tatgctacta tattttcaaa attgtgtgct ttaaaactag ctttttttag taatttaaag
    19561 cttaaaacta aggagactcg ttatatgaaa ataaatataa aatcaaagtt aagtgaattt
    19621 attaaacaga acaatctttt tgatgacagc agaataatat cctatcttcc taatataaca
    19681 atagaaacag acaaaataaa aactataatg attaatgagg ttgtcccaac taacaccaat
    19741 gacgattttt atagtgtaga taaagatgct gattatttaa aaactactat tcctttattt
    19801 gacagtgcag gaataaaagt atctaacata aatgatatcc tagatatggg tatctatata
    19861 actaatgcag taaaacttcc taaatcagaa tatacaataa ctcgtgatac aattaaatta
    19921 catatgccta ttttagaaga agaaattaaa ttatttaaaa acctagaggt agtaatgtta
    19981 atgggagatg ttgcaaaaaa atcttttaat atgattacta aaaagcatat aaaaaagaat
    20041 gtcatccctt ccatttcaac atataaactt cgtaataatg aactttatta tgataattta
    20101 agagtatttc catcatatat tatgactggt ggaaacatac tgattgaaaa atcaaaattt
    20161 gaaatggcat cagaagatat aaaaaaaatg ttcgagatta taaacggttg atattacaaa
    20221 ataataaaaa ctaatataaa ataaaaagtg cctcaaaaac ttctatttat gaggcacttt
    20281 tattttatat tagtgtaact atatactatt aacataattt attatttata ttgttccttt
    20341 taatctctat tccatttagt ctaggtaaca ttactcttta caagattact ttatatttct
    20401 tttatatctc taacgttttt atttaataaa acaattatta atacaagtca aatatatcta
    20461 tttatctaaa aatatatctt ttacagtaat atctggatgg tgatacttct tcctattcgt
    20521 taaaaaagat ttttttacat taatagcctc ttttggacac cagtgaaaac aagctaggca
    20581 tctttcacac tcattacccc actttggctt tttatcaact atacttatat tctctacagg
    20641 acaaaccctt ttacatgtcc cacatctatt acatttattt tctgcaaaaa atttagtagt
    20701 tgcaatttgc atattcttac ctactaaacg aagtacaaca ctatctatat gtgactttaa
    20761 attgtttttt ccttctatat ttccaatctc caagttattg atacattttg caatctcagc
    20821 taccctatta gtctgatttt tcaatctttc ttcttcaaca tctggaggtg ttatcaaagc
    20881 atttccaggc atattgatta aaaaaccagc agataatttt ttcccccttt ttttaagtaa
    20941 cttatctata gtaaataaac tatgtccagc taccccatta caagtggcaa tggcatatat
    21001 atgagtatta atattaaaat ttaatttctc tacaaaagac tttactatat caggaacatc
    21061 agcaaaataa acaggaaaaa caattaccaa tatatcttca tttatgtcta tattattttc
    21121 ttttctatgt atagctaagg gtataacttc tttacttcca tttaacgttt cttttaaaat
    21181 atttgaaact actagcgaat ttcctgttcc agaaaaataa taaatcttac tattcattct
    21241 tatccctctt tctatatttc tacatcatca atagttaaaa agtctcttaa aataggattt
    21301 atatttactt tccaattcta tccaaaatat cagtaaacaa aggcaattct tcatcagaga
    21361 tagatttaaa tgtctctgtt gcatatatac ttgcaatttc atttaatggt tcttctaatt
    21421 ctctacctaa atctgttagg aatatttgaa acttacgtct atctttaaca tctactttac
    21481 ggacaattaa tcccttattt ttcataacat ctaagattct tgtcgtatta gatttatcct
    21541 tacatgattt ttcacataaa tctatctgag aaattccatc ttgctcccat agaaacctta
    21601 ataacgcata atggtcagtt gttatatcat atccattttg tttaaacatc ttttttaaat
    21661 catcttttac ttttaacgaa gtacttacta acttagttgt agcttcatat agtttctcaa
    21721 aatctttcat tttacctcca tttaaataat cccttgctcc tcccatctat caattattcc
    21781 cttatattgc tcaatatctt taaaagccat tggctttaaa acagcattta tagcaataaa
    21841 attatagatt tgagaaagca caggtggatt tttcttttcc tctatgcaat aaataaattt
    21901 tttagatacc ttttgtatct ttgattctac tttttcagtt ataggatttt gattaaatcc
    21961 agtttttata gataaactac cacaaacata acctcccgaa tctaagacca tggattttat
    22021 aaaagaaatt gcttttgaac cacgtgcatt ttcatatgta gtaactgcta tacatggctt
    22081 acgatacaat aatctttcaa gcatcatatg tgctctatct gtaaacacct tgaaaagtcc
    22141 agttacacta cttccataag ttgggctacc aaatataaca ccatcactct tagtaattat
    22201 atcatgaata tactctactt tatcattttg attgatacac ttgcccattt tataacaatt
    22261 taaacaacca atacaatatg atggattgac ctcacttaaa tcaaaataat taatttttac
    22321 atctggtatt aatctttcta tatctttata caagtaagtc aaaacttttg atgtagctcc
    22381 attttttcta gggcttccat ttataactgt aattatcatt tctatatctc ctttttagtt
    22441 gttatatcaa caataagtat atcaactata tttttagctg tcaatataaa ccatttattt
    22501 aaagctatac ttacacagct taaataaaag ttttatttaa ctacatgtgc ttatttaatc
    22561 aactataaat gaattatttt taataattga caaactaaat taatgataat atactttatg
    22621 tattacaaat gtaagtagaa ggaggtatag tatgaaaaag ttaccacata tttcagaagc
    22681 agaatatcaa gttatgaaaa ttatttggaa atatgctcct atcagcacta ctgaagtaat
    22741 tgaaaagttg gtagaaacaa gtacatggag tcctaaaaca attcaaacaa tgttattgag
    22801 attagttaaa aaaggagctt taacatatga aaaaaatagc agagtattcg tttatactcc
    22861 acttgttaaa gaagaagaat acgtagctac agaaagtagt tcttttttaa atcgttttta
    22921 taatggtact cttaattcta tggtactaaa ctttttagaa aatgataaac tatctgaaga
    22981 tgatattgag gaactgagag aaatcttaaa taaaaggacg actaaggggg ataaataaat
    23041 atgtatgccc cattctttat acgattttta ctcagtacat taaccttatc aatattaatt
    23101 gttgtaattt tacttgccaa aaaagtattt aaaaaacata tttctgcaaa gtaccaatac
    23161 aatattggat ttatattgct tataatgtta ataattccat ttgtccctct taaatatatt
    23221 aatcttggaa atgtatttaa ctatttatat acttttaatc aacttggaag cagtactaat
    23281 aggacttcta tgataaaaaa tagttcaaat tctgtaatta ataactcaaa tgcattaaac
    23341 gatttttcag tatccctaaa tcaagttaat tttgatagtt taaatatttt attactaata
    23401 tcatggataa ttggaatttt aatcatgata gctattacta ttcattgcaa cattaaaata
    23461 aaaaatatga aaaaatcaat acaaatttta aaagatgagg agatagtatt tatatttgaa
    23521 aagtgtaaaa aagatattgg tataaataag gattttatac tatgcaaatc acctattata
    23581 aatacaccta ttacatttgg atactttaaa ccacacatta tattaccaga taagtctata
    23641 tctaaattat ctttaaaaga tataaaatat atcttacttc atgaattaca acattttaaa
    23701 aataaagata tactgataaa ttatataatg tgctttcttc aaatattata ttggtttaat
    23761 cctcttgtat ggtatgcatt taaagaaatg cgtatagacc gtgaaattgc atgtgatatt
    23821 tcagttttaa aaaagcttaa taaagatagt catatagaat atggacaaac tctaataaac
    23881 tttgtggata atatttcaag accatcaaat cttacactta catcagatat tggtggttca
    23941 aaaaaacaaa taaaaaagcg actattgaag ataatggatt ttcataatga atcaaatacg
    24001 ttaaaattca aaagcatttt aatttttaca ttgatagctt taattgtatt tggaaatatt
    24061 cccaaactat ctattatggc agatatcaac gaaaaatata gacctacaaa tgaacaaact
    24121 gtgtacgagg acttaagtca gtattttaaa ggttttgatg gaagttttgt tatgtatgat
    24181 ttaaaatcca agcaatatca tatttacaat aaagaaaaaa gtaaaacaag aatttctcca
    24241 aattctactt ataagattta tagtgcctta ttgggattgg aaaatgggat aataacaaga
    24301 aataattcac atatcccttg ggatggaaag catcattcta atagttcttg gaatgaagac
    24361 caagacttat tttctgctat ggaaaactcc gtaaactggt actttcagac tctagataaa
    24421 aaaacagggc taaaaaacat acaaacttat cttaggcaaa ttggatatgg caattatgat
    24481 gtttcaggtg gtatctcaaa cttttggcta gaatcttcat taaaaatatc acctattgaa
    24541 caggtagaat tacttgaaag tttttataca aataagttta aatttgaaga taaaaatata
    24601 gaaacagtaa aagattcatt gttaatatca aaaaaggatg gggtatcact ttttggtaaa
    24661 acaggcactg ccaatataaa tggaaaagat gtaaatggtt ggtttatagg ttatgtggaa
    24721 aaaaatgaaa atacttattt ctttgctaca aatattcaaa gcaaagatta ttctaatggt
    24781 agttcagcat ctaagataac attatccata ttaaatgaca aaaaaatcta taatccttaa
    24841 tatatttact gtacattcta aaaaattcac ataattttca tattaattat caattaaatt
    24901 catatacaag gcttattata taaacataga agatagtaaa actagcttaa aaacataata
    24961 taatacccta tacaaatagt ggcaactctc cctcacatcc tcaattgttg ccactattta
    25021 attttttgta tgaagttaaa tatattttca tcatttcaac aatttgggtg caatacaatc
    25081 tctattttga aacaacctct ttaattgttt tagagtttat gaattatttc tcatacttta
    25141 aaaagattga attactcttg ataatcttct tttgtagtca ttacatatca cctactactc
    25201 aacacatatt ctttgaccta tttttacatc cttgtttata tccaaaatat ttaccttctt
    25261 accatctgga aacaatacca ttgtcaaagt agactttcct aattcatgta ttctctttaa
    25321 atcaacatta acaagaacgt ctccctgttt aaccatatct ccttgtttaa cagcaacatc
    25381 aaagccatct ccatttaatg caactgtatc aattccaaca tgaattaata cttctccatt
    25441 agttccttta atcccaaatg catgtccact tgaaaccact gtttctaatt tacctgaaat
    25501 aggagaaaca atttttccat ctataatatc aatagcaaaa ccatcaccta acattttttt
    25561 agaaaatgtt tcatctggta cattttctaa tgcaacaatt ttccccttag ctggagacac
    25621 aaagttttca ttattttcat taaacactaa ttcttcgtct tcatcttcat ttatagcttc
    25681 tttaggcact ccaaataaac atgttacaat aaagcagaat gcaaccacaa atattgatac
    25741 taataagtat gtgattaaat tatatccatc atagatatac attaaataag aaggtaaaac
    25801 tgctaatcca tatgaattag attgaatatg taaaattgat aatattgctg ctcctaaacc
    25861 tgaagatgca cacataataa tcaaaggctt taaattatat cgaagctgaa tcccaaataa
    25921 aacaggctca cttataccaa aaagttgaga tagcattgct ccaaccgcag tttgtttaac
    25981 ttgattattt ttagattttt tcataaatgc tagacatgtt ccaatattcg caaatccata
    26041 cattgcacaa agagtaataa gggcattaaa tcctgtattt gctaatagtg atgtttcaat
    26101 cattacataa gtatgatgta agccagtaat aacagctaat ggatatgtaa atccaataat
    26161 aaatcctcca attcctaaag gcaaatcaat caataagctg atgatatcaa ccatttttaa
    26221 ttctattgta tgcattatag ggcctacacc taacaaaata actaacattg ttattagcat
    26281 aacaaaaaat ggtgtgaaaa taaggtctaa tacattaggc atatgtttac ggaaccattt
    26341 ttcaagatta gctccaataa atgctgtaac aatagctgtt aaaacacttc cttgacaacc
    26401 tacaatagga ataaatccaa atgccataat tgcttcaatt tcatttgatg ggtcagcaac
    26461 agaatatgca ttaggaagta ttggggaaac taacataagt ccaataacaa gtccaataac
    26521 aggtgttcct ccaaatactt taaatgctga ccatacaata attgctggta aaaaagcaaa
    26581 tgctgtttct gtaagaaccg aaacaagtgt ctgaatatac aatggaatat ttgcagaaga
    26641 cattccaaat aatccaagta cattatcatt aaacaaacaa ccctttaatc ccaagaataa
    26701 tcctgttgct gcaataactg gaataatagg aacaaatata tctgctagag ttctcataac
    26761 tttcttaaat ccagtttcat tatttttaac taattcatct tgttcttttt ttgataaagt
    26821 ttttaatccc attttttcaa cttctgcata aactttatta actattcctg ttcctaaaat
    26881 aatttgatac tgtccagaag taaagaaaac ccctttaacc tcatcaactt tttcaacttt
    26941 cttatcatta ataatttctc tatctttaac catcactctt aatcttgtgg cacaatgtgt
    27001 aatagaaata atattatctt caccaataat tttaataagc tcatttgcta ttttattata
    27061 tttattcatt tttctacctc actttcaata atactgcatg aaatggtttc aaacgcactg
    27121 ttttatttaa tagatttata tcatctaaat tttggaatac aatatcatat ttttcttcta
    27181 ctcttatttc tacaggttta tcagaaaaat tataataaca atcaataact accaaatcta
    27241 attgccttct atacgcaata atatcatcac tttctatatc aataggttct attgaaccat
    27301 aaatcaaaca atcagaatac tcactatctt gtctcaactt tatcatcttt ttataaaatt
    27361 ctaaaatact ctctgaattg ttaatctgac tctgtgcatt aatatcttta taattttcaa
    27421 taacctttaa ccatggtgtt gtatctgaaa aacctgcatt ttctttatct gcccatggaa
    27481 atggcgttct tgcattatct cggcttctta aattcacaaa ttctaacgct tcttcttctg
    27541 agtaactttc atctattgaa cgatagtatt ggtcaatgct agataaatca ttaaaatcat
    27601 ttatggtatc tcttttaaaa tttgtcattc ctaattcctg tccctgataa ataaatgggg
    27661 ttcctcttaa aaagaaatac attgctccca acatttttac tgcatcatca ttcctttcgt
    27721 attgcaaaag atactttgtt gtagctcttg gttggtcatg attttcaata aaattagctg
    27781 cccatccatg ttcctgaaca tacatttgac tatctaaaat gcattttctt aaatctggaa
    27841 ttgtccatga aattctttta aaccactcac taccagaagc aacatctaag tcagcatgtt
    27901 taaagtcaaa gaccattgaa aagaaaccat cttctccaat aaaatcacct agctggtcat
    27961 agctaatacc tgctgcttca gcaacagtca tacaatcata ttttttaaac gtattttctt
    28021 ttaattcata aagcattttt tctattccag gctgatttct aactgttttt gtacatttaa
    28081 caagaccatc aacaccatca ctttctaaac tttgaaaact taaattcttt ttgatgaatg
    28141 taatagcatc aactctaaat cctgccactc ccatttgaga ccaggtatta atcatttggt
    28201 aaagttcact tcttaatatt ggattttccc aattaaggtc aggttgtttt ttaccaaaag
    28261 aatgaaaata atattcgtct ctattctcta ccttttccca aacacttcca ccaaaaacag
    28321 acctccagtt atttggtaaa tcgtttcctt ttttaaaaat ataataatca tgataaggac
    28381 tttctggatt ttttaatgct tcttgaaacc aaggatgttc atcacttgta tgattaagaa
    28441 ctaaatctaa tataattttg attcctctat ctttactttc tttaattaac tcttttaaat
    28501 cttccatact tccaaattca cttgcaatat ccaaataatc actaatatca tacccattat
    28561 cgtccatagg tgatttaaaa attggacata accacaatga tgtaatacct aaatttttta
    28621 aataatctaa acgagaaata attccctgta aatctccaat tccatcatta ttactatctt
    28681 gaaagcttct tggataaatt tgataaacga tttctttttg ccaccatttc ttttccataa
    28741 attactcctt tttatatgat atttcttctt tttaattctt tataaattcc attatcgaat
    28801 atgtcctcac atatagatgt cgctatatct ttaagttgtt gatgactatt tttcattgca
    28861 attgtaacgt ctgatgcttg aaacatgaca acatcgtttt gaccatcacc aaaacatatg
    28921 gtttcttttt gagttactcc taatctttct tgtaatctct taattgcttt gcccttatga
    28981 aaatcttttt gaataatttc ataatactga ctagaaatat ctctctgtgc taattccatt
    29041 ttatcttgta gtatattttt cacttcgtca aatacctttt catttgacca taaacaaatt
    29101 ttatgtatat cttgtgactt atactcttca atgttatttt catatgttat tttttcttgg
    29161 atatgttgtt tatttataca agaattagtt cctttcaact gattcattgt ttcaaatatt
    29221 tctttggctt tttgattcat gaaaactttt tcctgactct caatcgaaaa agcaacttct
    29281 ctctttttta ataaacaaac aacttccttt atcaatcttt gattaaaaga ttgattataa
    29341 agtaattcac catgatattg aatgtaatta cctcctcctg caatatatcc atcaactcct
    29401 aatgataaaa catcatcttg tattgtaccc atacttcttc cagtacaaat aacaacactg
    29461 caatgattct tttgacataa acgtatagca tgctttgcag attctgggat tccatatacc
    29521 tcatctctaa gcgtaccatc tatatcaagt attaataatt tgtacaattt catcttcctt
    29581 tcttactatc aaaatcataa tagcattcaa aaaaaaagat atcaatattt tgatatcttt
    29641 taaaaaatat gttcattttt agcacaaatt ttcattttcg gggaatatgt tcactttcta
    29701 aatacatctt gtatttaata taaagcagtt caattaaaat aaaataaggt gttgttacat
    29761 cgtagtccag ttgtttatct acagaaatag atatagattg aatatacagg ttttcatcac
    29821 ataatcttgc taaaggatta cttatcattt ttgtaataga aaccatataa attcctttca
    29881 ttctcatttc tttagcaaaa tcaataattg tagatgcatc tccattcaat gaaataaaga
    29941 aaatcatatc atctgttttt ataaaatgat taagtgcctg aatcatatca taaccataaa
    30001 catcatatat aattttccct gttggtaaga aaatcctttt catctcttta gcaactcttt
    30061 cctgagaata accttctcca aagacgatta tacgatttgc ctttttcatt ctttcaaaca
    30121 taatcgaaca atccttcttc tctatatcat ctatcacctt attataacaa tccattaatt
    30181 ggtgtttact ttgattttta tattctactt ctgtttcatt taagcgtata ataactctta
    30241 attcactata tccaggtaat tgtagttttt gagcaaaacg agaaagtgca cttgtagata
    30301 tatggtattt gtctgcaaat tcatctatag aaagtttaat acaatcttct ttatgattaa
    30361 gaatacactc acagatatac ttatcatttg ctgtaaatga attataatat ttattgaaaa
    30421 gtccttcaat tttcattctt ttctccttct atactctatg taattcatat gaagcaaatc
    30481 taatagagca taaaatgctc ctacgacttc ataagatata gcttgtaatg tatcaagttt
    30541 ttgcgtttca acatacagat tatactcaca aagtgttgca attgtattat tatcaagcct
    30601 agttaaagaa atcgtatgta tatgattgtt gataaatttt gcaatttcaa ttcctgcttt
    30661 tgtttctcca gacaaagaaa tgataataaa taaatcattt ttttgaaatg attctctcat
    30721 aaattcaatt tcacctatat caaataagtc aataacacat ttacctactg aaagaaatat
    30781 tctctttatt tcttgagcta aacttttctg ttcattacct gtcccataaa tataaattgt
    30841 ttctgcatca tatattattt tacaaatatc ttgaaatgat attttttgta attcattcat
    30901 taatagtcga taattctcgc aaacatcatg aaaattatta tcattattaa caaattctag
    30961 ttgattattt ttaagtaaat actttaaatc agagaatgaa tctaatccaa tttttctaca
    31021 aagccttaat aaagtagctc tagaaacatt acattcattt gcaatttttt cacacgaaaa
    31081 attacggtat tctctttgac aaatcaatat ttgtttaata ataattttat cattttcatt
    31141 taaattatca taattttggt taactaacga ttctaatctc attttttcca ctccctaaat
    31201 attattttaa tatacattag ttgctttttc tagggaagag ttttaaataa taaggataaa
    31261 ataatcattt gattttattt ttattaaaat aaaaataacc tgctaaaagc aggcaccaaa
    31321 aagtacttat cctttcgaac atgtacttta gatattgctt tcttcggaag cttcatctct
    31381 tcatactcta aaatatagtt ttttctaact caaaagtatt ttccatggta gaaattactt
    31441 taattcatct gccatttact ttttatctta tttaataatt actattactt gaatatttac
    31501 ttctttaagt aaatttcata gttaacaaag ctaaaactat tattatagat gttaaaatac
    31561 aaaattgtaa gtattgttat aaatatgctt acagctacaa atatacatga tatattaaaa
    31621 agaagctgta aattattatc atacatacaa gcaactatgc ctctattaat ccatgttaat
    31681 ttttattatt taatacaagg agttccatga gacatacata tccattcata ttttaaatta
    31741 tccaattctc ttatagattt caccatcttt ttataatccc aattccatgg attaggataa
    31801 ggaactatat ttccattctt atttttgact aaatcacctg caaataatac agcttcaaaa
    31861 atcatgcaga catgccctgg agtatgacca ggagtatgga taatttttat attcccaatc
    31921 tgcatattct ctccataagg ttcaacatcc ttaatgagcc ttctggaaat tgcctttcca
    31981 ataaacttct taaacccagg tctatccttc tctcctataa taaaaggtat atcctctaca
    32041 tgagcccata cttttgctcc tgttttttct ttcaataatt taatattaga tatatggtca
    32101 acatcatgat gagttaataa aatatgctta atatctgtaa gtttaatgcc aagtgttttt
    32161 aattctttaa ttatattttt ccccataaaa ggaagacctg tgtctataag aataatttct
    32221 ttatcaaaca caataaaaca atttgagcca cgagtactat ctaacattaa tacattttct
    32281 tttatcttca taaataaatt ccccttattt ggtattttac ctatataatt aattaataaa
    32341 gttataaata gttataatac ttgatattaa ttttattttt tattaaagta tactataggt
    32401 atacaagtat tatatttaca taagtatacc atcagtatac ttttttatca atagaaagga
    32461 ataatttata tgtcagaatt atcaaaaaga gataaagaaa aaatacaaag agaaaatgag
    32521 attattgata aagcagaaaa attattttgt cttaatggat ttgataatac aactatgaat
    32581 gaattagcaa aagaagttga atatactaaa cgcaccatat acaagtactt ttcatgtaaa
    32641 gaagatttat tctttgctgt tgtcttaaga ggatataaaa gactttggga taatgttaaa
    32701 atagaaagtg caaaaggcaa aacagggttt gaaaaaataa aactttcata ctttgctttt
    32761 cataaatttt actctgtaga accttctcta ttatgcctta tgggaatgat tggtattgta
    32821 aactctgaaa aatcggatac tgatatgctg tttaaagaaa agtttttttc ttttaataaa
    32881 tttatgtttg atgaaataca aggaatgttt gaaattggaa aaaatgatag aagtattagg
    32941 catgatattg aaatacctac tcttatgtat tcatcaatat ttacacttac aggatttttt
    33001 aatttactat ctgtaacagg aaaatcatac ctgaacaact ttaatataga tgaggaaaaa
    33061 tttattgaaa caactcttgc tcttttaata gattcaatta aagcataaac ctaagtttat
    33121 aaattaaagt ttatatactt aaatgataat tttttctatc tctaatataa aaaatattaa
    33181 acataaaact aataacttag ttaatggaat aactaaatta ctgaaaggat taaaagtaaa
    33241 tggctaaaat acttctttta gaagatgatg atacaattac atttggaata aaaacttccc
    33301 ttgaaaagaa aaatcacact gtatgttgtt ataatacgat tgctgaagct aaaaaaaatt
    33361 ttgataaaac tattgattta attctgcttg atttaaatct acctgatggt attggatacg
    33421 agttttgcca atatgttaaa agtatagaag atactccagt gatatttttg acagtccgtg
    33481 atgatgaaaa agacattata cgtggattag atatgggtgc agatgactat gtggttaaac
    33541 cgtttttgct ctcagtactt cagtccagaa ttaatgcagt tcttagaaga actcattcaa
    33601 atactaacaa tactattaca tgtgaaaata taagcttgaa taaggttgaa gtcaaagcat
    33661 atttagataa tgttgaagta cctttgacag caggagaata taaacttctt ctaagtctta
    33721 tggaaaataa aaatagagct atgactcgta caaagttact agaaatgctc tgggatgtag
    33781 atggtaattt cgtaaatgat aacactctta ctgttacaat aaaacgtctt agagaaaaat
    33841 taaaaaatcc accttgtata aaaacgttaa gaggcatagg atatagaatg gaggacagct
    33901 agtgaaaact ccaagaaata tcaacagtac agttgttttt ataatattct gcttaattgt
    33961 aactttttta ttgagtgtaa ttctaacaac tataacttct tttaatgaat acaatgtact
    34021 gtctaaggct cttggtgcat catatgagta caatcctaag tctacagaaa gtatactaaa
    34081 atcactgaaa aattctagtg ataaggattt taaaacagga aatgaaattt tatcaaaaca
    34141 tggatattct aaaagtacat tttggagtaa aaactttttt tattttctag tgatatcgtt
    34201 tggtataatt tcaacaatgg ctacagttat atatataatc aaacatagaa caaataagca
    34261 aaaactgagt agaataaaca atcttactca atacttagaa gaagtgaatt taggaaaaga
    34321 gtctgtactt ttacgatgtg aagatgaatt ttctcacctt gaagatgaga tatataaaac
    34381 agttggggaa cttcgttgtt caaaagaatt tgctttaaaa gaaagacaaa ctctttcaga
    34441 taatcttgca gatattgctc atcaacttaa gactccaatt acttctatgt cacttatggc
    34501 acaattactt tcagaaaatt gtccagatga agaaatagat tatattaaca gacttacaaa
    34561 tcaaatctca agacttgaga gactagtttc atctttactt acattatcaa aattggatgc
    34621 aagtacttta atatttgaaa gcaaacttat agatgtacat tctcttctta cttatgcaat
    34681 tgagcctata gaaggttctt taagagaaaa acatcagaca tttactatcg aggcttctcc
    34741 ttcggtagaa tttgaaatcg atattaattg gactttagaa gctcttttaa atattcttaa
    34801 aaattgtagt gagcatatag aagatggtgg atatatagtt gcttattata gtcaaaatcc
    34861 tctatatgtt gagattatca tagaagataa tggtaagggg tttgctcagg aagaacttcc
    34921 atatattttt aatagatttt atcgtggtaa aaatgctagt aaggacagta ttggtatagg
    34981 gcttgctcta tcaaagtcta ttatagaacg tcaaaatggt actctacatg cagaaaattg
    35041 ccataatggt ggtgcaagat tcataattaa attttacaca taaaatgctg gattataggt
    35101 aaaataccta taatccagct atatctatat tctatgttat ctattgtttt agtaatatac
    35161 ttctatttac atttaaggtt ttattaaaca aacaacttaa ctatccctat acttgtaagt
    35221 agtcttaaat cgaaaccttt aaataagttc aaatagattt tgtttatatt gtttcatttt
    35281 ttatagattc tactatattt tcattcttca actgcttgta tcctgtcata tatgatgcta
    35341 ttacaataaa aagtatcaat atagtaaaac ctattattgt agatataggt gcaaatggta
    35401 aatactctat aaaatatatt tcatttatac gtagaaaagc atacactatt cctatttgca
    35461 caggaatact aagtagtaat ggtgtaattc ctaagaataa accttccaaa ataagcatct
    35521 tttttatgcc ttctggagat aatccaacag aacgcaacat agcaaactct tgtcgtctac
    35581 ttctaatatt tcctgacact gtagcaagta catttgataa tccaatcata gccaataaac
    35641 ctgtaataaa gcctactatt aaattcatgg tctttattcc actagccttt tgtttttcac
    35701 tttctaaaac atcctcaata ctataatctc ctgaaccata gtctttatta taaatggact
    35761 ctaatttttc tcttactcta ggaatgtcct cacgattatt tactttaaat actcctatca
    35821 gcctatttga acgtaaccta cttacttcat cataattttt agatatttcc tgtaccatag
    35881 acattggaac tatttgcatt aatgtatact ttgaactagt atctactatt ttaggaagtg
    35941 tgcttgtaat agctcccact ttcatgttgt aagtataatc ccctttatct tcgtcatata
    36001 ctttttcatt taatttaatg ttatctccta ttgaaagatt taaataatct atatatactt
    36061 tatttctacg tgtactacga ttaatatctt cctccttatt atatacaatt gaggttggat
    36121 tttttgtatt ataaaataac tttgggtcta cagaaatttg cctacaatat tctgtaaaac
    36181 tcttatcatc aagcccaatt atatttgaaa ttatacgaaa cttgccatca cgttctatag
    36241 gagagtattt tttagatgaa actatacttt taaatcctcc atttttcgca agttcttttg
    36301 atgcatcttt ttcagtcagc caagtggaag cagacatttt ttgataatat agtgaacttt
    36361 ttacaccttc tgtattatct aatttttcaa tgaaagcaga ctctataggt tcactatttt
    36421 ccaaataaac tgctatatct ctttggtcct tataattatc acttccataa acagcttttg
    36481 ctccttcttg atttgcaatt atatgcaaaa aacatgtaag taataaaaaa gataatgtta
    36541 aagatattgt tgcagtacga taagattttt tacgagcacg tagtgcgttt gatgcaagtt
    36601 ctccttctat tccaaatatc tttcctaatg aatatttatg cgactttttg attttaacac
    36661 tatctccttg tcgtattgct tctataggag atatctttga tacttttcgt gctggtataa
    36721 gtgcagaaat ccagactgtt actattgtaa gtaaaactgc tggcagaaaa gcaggaatac
    36781 cataagtaaa taccacttct ggaacatcag tatgataatt aactgaatta acataaacta
    36841 taagcctatt acacaataac catcctaaaa ataaacctat tggtaatggt ataattgtca
    36901 gtaaaaagcc ttcaaacact acagaccttt taatttgttt tggtgatgca ccaacactag
    36961 ataatatccc taactgagta agtctagcat ttgcagacag tgaaaacgcg ttgtaaatca
    37021 ccattacaaa cacggctact gtaaaaattg ctattgtaag atacataata ggagttgaaa
    37081 attttgatat actactcata gatgctttct gttctggtga aaagactaac atctttaaaa
    37141 gataattact attgtattta agattgtatt ttccatattc attagtcttc catcccaatg
    37201 atttagcaag ttttggaagt tccttatacg tgtctcttat atttttaaat cgcaagtaca
    37261 cagttatact atcattaggt tttatacttg ttttatcaag atatccaaga gctgtatatg
    37321 caggtattac tgatgaagta gaaacatcta cctttcctac tattgtcaat gtagcctctg
    37381 aaattttttt aaaactttcc cctttcgatt ttggggctat gggttcaaga ggctttccat
    37441 ttaagattct atatcctatt gggagagtaa ttttatcacc aatttttaca cttggattat
    37501 tctcaaaata ttgttttgat aatgcaattt cattagcatt atttggtcga cgaccctcta
    37561 ttatagtatc cttttctggc atagaattcc aatactctga tgtagcattt ctataaatta
    37621 tataatctct tttctcatca ttaattttgc ctacatacca ttgtcctttt accattacat
    37681 catcaactga agaaaaattt tcaattaatg gtaagtcact tccataagta tagtcaaaca
    37741 attcaccatg ccagttacct tctttctcaa ttgaaagtct tataaaatct gtccacatgg
    37801 tatacaacag tccacacaaa gaagacatca tggttgtagt aagtaatata gctaacatta
    37861 tagctatact actacgttta ttgcgtcgta caaagtctaa ggtgtattct tgaagaatat
    37921 tcatttcttc accacctttt cagagacaat cactccatct tccatagtta caatacgatt
    37981 tgcctctagt gctattttct catcatgagt aataagaagt attgtctgat taaatcgttt
    38041 atttgataag tttaacaagt ctacaatttc ttctgaattt tttctatcaa gattaccagt
    38101 tggctcatct gctaataaaa tagctggtct atagattaaa cttctagcta tagataccct
    38161 ttgttgttgt ccacctgaaa gttgacttgg aagatggttt aatctatcag ataatcccaa
    38221 tatagataca atctcagaga atctatcctc atccaccttt cttttatcaa gtaacatggg
    38281 taataatata ttttttctaa catcaagggt aggtataaga ttataaaatt gataaataag
    38341 acctactttt ctacgtctaa aaactgcaag agcttcttcc tttaaacttg atatttctgt
    38401 atcttcaaca taaatctttc cagatgtagg tctatctact cccccaagca gatgtaacaa
    38461 tgtagatttg ccagaaccag atgctcctac aatggataca aaatcacctt tttgaactga
    38521 taaatttaca ttattaaggg ctgtcactct ggtttgattt gaaccatata tttttgtgag
    38581 attttcacat tttaatattt ccatatacaa tcatctcctt tatggttaaa ttttagcaac
    38641 ctaaaatgac attatagtga catgaataca aatataaatt actattaaaa atattgttta
    38701 gtttttgcac attatttaaa attataatat aaaacaaaaa ctatgcaata tattttattt
    38761 atacttttaa tatattacat agtctttaaa aggtattaac tttaaatcta taggtattca
    38821 atttaataat caatagcaag tattttatgt actaaaggag ttttgctcta tatagactca
    38881 atttagtaaa attatttaat aaatattaca gactataggt aaataatatt tacaaataaa
    38941 attaagttta ttcccttaca ctataatatt tcttaattat attaattgca atttccttta
    39001 cctttggccc tgtaatttct ttattggaat catctgattt aatagcaaaa taataggtgt
    39061 caccttcacg ctctagcata ccaacaaacc atgcatccac acgtttattt ttttcatcaa
    39121 atcctgttcc agtttttcca tatacattta tgtttttatc atttatatct attttcataa
    39181 tatctcttaa aatattaata tgctcttttt taaagtttgt atctccctca aatatttttg
    39241 ccattgtttg cacttgttct tttggagata tttgtaatga agattctaac caaaatccat
    39301 ttaaatgtcc ctttccattt tttaaatcgc cttcccattc gctaatatcg caattcccat
    39361 actttaaatc atctaaaatg ttttggacag atttagcatc aactttatct atcaattttt
    39421 tgtaatacca aacacaagac tccttaaatg cctcttctaa actaagattt ttattccaat
    39481 ttttatttgg atactctgta ccatcatatc ccataactga ttcttttgag tttattaccc
    39541 ctttttctaa accaattaaa gttgaaacta ttttaaaagt agaacatggt gaccttcttg
    39601 tttcaattaa ttctttgtta tatatattat attctttatt tttagtatta taaaaaatag
    39661 ctcctccact aataccttca aaacaatcac tatagtcaac catattaatt ttttcttttt
    39721 tgtttttatc attgttttca attttggatt tatttaattc agacttattt aattcagatt
    39781 ttatataatt cttatcagtt ttttgattta tatcgttatg ttttttccct acataataca
    39841 ttaccataac tacaccaacc aataagatag ctatccaaat aaaattcttt tttcttttca
    39901 taatttatat catctcccat atttaaattt ataacctact attaaaatag tagacattat
    39961 ttgtaatata ctccaatcta ggttctactt ataagaaaca agactaaatg aatagctgta
    40021 tctcttatat tttcttcacc tagtacaatt atgttgatta aacctattgc gaagataatt
    40081 aggtaactat taataacatc ctgtcctcct tttaatttgt gatataataa ttgattccac
    40141 atttaaaact tactattgta atatttaaat attacaatag taagttttaa atgtcaagtt
    40201 agtttaggaa agttatttta attgtataat tgttattaca tttataaaat atggcatgtt
    40261 taaaattatt ttgctattgg gaggattaag ataagcagta ttataaaagc tataaattat
    40321 aaaatttaat atataattta tagcttttac ttttacaaat attgttttta ttgatttatt
    40381 aatgaatttt attgacaggt tatatttata taagtaaaca cttacataat aagtatataa
    40441 tctacatatc tataagttga gtcaattcac aagtatatgt caacttaagt ttatgcatag
    40501 ctcgtgtaca ggcaatatat aacaaactgc ggtcataatc actataatat gttttattat
    40561 ttactgaagg tacaataact tcatcaaact ctaaaccttt tgacatttta atagaagtta
    40621 tggatactcc ttttgtaaag ctagaacttt cagaagatat taagtgaaca ctatattctt
    40681 ctcttaacgc atcatataca gctttagcgt cattatctgt ctttaatata ataccaagag
    40741 tcgcattatc actcttttta aattcttcaa tttctatttt tatcttattt atttcatctt
    40801 gctcgttatt acattttaat aaagctggtt cttcaccatg tcgttcaata ggctctaatg
    40861 aagatatgtc ttgaatcttt ttggcgaaat taataatctc aaaagtagaa cggtaactct
    40921 tgttcaacat aactaattcc ccatcattat atagctgttt catatcatct agtgtatgag
    40981 tatgattagg attaaccaat tgtccaaaat ctccaagaat agtcttttta cacttaaaaa
    41041 gtaagtttat tacagcatat tgaataggtg tatagtcctg catttcatca ataacaatat
    41101 gccttataat cttatcttct tgtatacctt catatgcagc atgaatatat ataaatgggt
    41161 atacatctga ccattcaagt gttttctttt ctgccatcac aaacatgttt gatatattca
    41221 tttgtttaaa gaaatcctta tatagtgtaa gagtattttt tattgtcaac attccattca
    41281 aacttttaag tatacttttt acctttggta aatcttcttc cataatatta tctgattcaa
    41341 atttataatg aatgtcttca gctaccttct ctaatctctt cttaactgga tatctgttat
    41401 atgcctcaaa tctactctgt atccagtcac ttttagctgt aaaacttcca aaagtgtagt
    41461 cttttggaat aaaaatctta tttggcattt gttttatata atcatcaata agttttacaa
    41521 aatcaagtgt agatttaaaa cgcactcttt cagaccattt agcatcattt atttctaatg
    41581 ggtctttctc actttcaaaa ttaataactc tatctaattg tacctctgct atattttcaa
    41641 aacttaattc acataatggc tcttctccaa gctcaggaag gacattggaa atatagtctc
    41701 caaagacttt attaggtgat aggataatta cattatttgc tgatatttta tctttaaatc
    41761 gataaagaag aaaagcaatt ctgtgtaatg ctatagatgt tttcccagaa ccagctaccc
    41821 cttgaataat cagtgtatca gctttatcat ttctaataat ttgattttgt tccttttgta
    41881 ttgttgaaat gattgacttc attttttcat ctgaagtgtg actaagttct ttttgaagaa
    41941 tatcatcttg tatattaatg gaactttcta gtgcatattc taattttcca tttttaattt
    42001 taaattgtcg ttttcttgtt atttctccat ccacccatcc tattggagca tcatatccag
    42061 cttttccgat ttcataatca taaaacatac tggcaattgg agaacgccag tcaagtatta
    42121 taagctcatc ttcataatca aatgcaaatc taccaatata atacttagat tcatcatcca
    42181 tatctttcaa cctaaaatct attctagcaa agtatggaga atctttgatt ttattaatct
    42241 tatctcgaac ttttaccata aaagccccat agttatctat ttgttttagt ccttgttcat
    42301 tttgaaacat ttcatgtggg tctatttcac cacgattttt taccatatac attttagcat
    42361 ccatataatc cttatcaagc ttgtcaacaa gattttcagc tttcttaaaa gcattgtcta
    42421 gcttgttatt tatctctatt agatgtgcaa tctcatctgg aaaaaaagtt aataactctg
    42481 catcatattc acacatatta tgtttagaat tagatgcaat aatatcgcaa tttactactt
    42541 gtttattttc agacatttaa atcctccttt gttaattaag tacttacact gaatcattat
    42601 acttaattgt gttttggagt ttttaatcat tcttgctatt taacttaaag gaagaataaa
    42661 tttttgagta ggatatccaa attccacaag caattctgct tctgcaaagc ccaacttctt
    42721 tatcatatct ctatgtcctg tatcagcttt gtcaccctct cgaaaggtag taattgaaat
    42781 atctgtatct atcaagaaat cttcaataac tttcttcaaa aacacttgtg caatgtctga
    42841 attacgatac tgtggatgta ttccaaaaaa atcaatactt ctagtttctc tattaaacac
    42901 catatttgca atagcgacat tattaagttt taatattaaa gctctttctt ctaaaatata
    42961 ctgttctaat tgatatatat attcttcctc ttgcaaattg ggaaatccat ctatgactaa
    43021 tctcactaaa ttcatccata atggtatgtc agacatactt gcaagtttaa tttctttttc
    43081 caactctagt acattctttg aaaataggtt atcttttttc aaaacaaatc ttagctgcaa
    43141 aggataaaat tcttgttctt tcctatattt atttggagat tttttataca tagctttaaa
    43201 tatagaagtg aatgcctgtt gactttcata tccagctatc aatgcaattt caataattgg
    43261 tttctttgaa aataccaata attttgctgc ttctgtcaac tttctccgct ttatgtaatc
    43321 atgcattgtt aggccaacag aagttgtgaa agttctatgc aggtgatatt ttgagtaatg
    43381 aacagcatta gcaactatat ctaaatccag cttttctgat aaatgctcct ctatatagtc
    43441 aatagcagtt atgacattaa ttactttgta agacattgca gcttccctcc ttttatccat
    43501 gcaacattat atcaattatt acttagttat atttaatgaa tcttgctatt ttataaacaa
    43561 atttgttgtt taagttttca attcataaat ttaatatatt actttttact ttctgattaa
    43621 agactctaat cctttaattt tcaataactt ttcatttagt aaatctaaaa agttatttat
    43681 tattttcttc ttattagatt ccttaattaa atgataaata tttatattat acgactaaaa
    43741 acagcttttt acaattattt aatatgatta caaacttacc ctatcttaaa gtttaatggg
    43801 gtcctgttat gaatcttttt attttaattt aagctcttca tcagtttatt ttatatattg
    43861 atgtatttga tttcatacaa aaaagagtgc ttcatgaact aaaaagttca tttttaaaca
    43921 ctctctttta tgtaaataaa tttctattct attttgaaat attataattt gacttataat
    43981 ctattaatac caaaaataat aaattgtacg tctaagaata gattctaatt agttgctcag
    44041 tagtaaaatc tgtttttaac taatcctgct ttgtatgttg aacatattct ataattgtac
    44101 catctggatg tctcaatgtc atattatatc ctgtaggaac ttttttctta tctcttacta
    44161 caactgctcc attatccaat agataacttc tccattcatc aacagaatca accataaaag
    44221 tagattttgt ttctataaat ggtttaagag catcatcaga accagataat agtaaaacat
    44281 ttcctatttg cgctaattcc aacccaactt ctttatagga aaaacgcaaa gaacattttt
    44341 cttttaaaag attctcataa aaaggtatgg ctttttctat atcttgaata taaaaacgtg
    44401 ttaatatatc atatattttc atattttccc cctatatatc tatataatat tacctctaaa
    44461 ttataccact ctacaaatta tataacaata aattaagcat agccttttga gcatcttaag
    44521 tagaccttaa caatatattg gggttacatt agaatacata aattaaatca ccttgatatt
    44581 atttaggata aaatttatgt agtagcagta aatgttcttc taattatcaa ctaacaaacg
    44641 ataatcttta actttctata taatgggtaa catcattttc gtaagatatt cactagctgg
    44701 acgattgata tcatttaaat actggtaata tatatcgcca tgaagtttat atccattgct
    44761 atttgcccat gaaaataagt cttctaaagt agggtcttgt aattcataaa gtcctaaatc
    44821 aattgcagtt attattttct gtgcaggaac aatcttttgt agaatctcat ttttcccatc
    44881 tatataagtg gctacaggaa accctacttc aacatccaat tttactaaat ccatgttatg
    44941 aaaacataca atcggtgctc ctgaagataa aattcctctc tcctccagat atttagaaat
    45001 tttttcaaaa ctttgttttg aaaactctga aaattctacc ataaagtcaa ttgtttttcg
    45061 tattgccaaa aaacagtact cagattgttc agctattgtt atattggata ttctagtcat
    45121 tttattcctc cattacttta ttttcatttc taatcttttt ataccaaatt atacgaaaat
    45181 tcactgtgta actaattatt tcatatgaat ataaaacata acctccttcc atttcttata
    45241 tcgaaagtat agcaaaaata atctcatcat tcttgtcaca cagtgctctt ataatcagaa
    45301 catacttaaa agacttgcct aatttttagg caagtctaaa agaaatcttt ttctataaaa
    45361 aatttataca aacaattaaa atatcaaaat atagactaat ataaaactat aaatttacta
    45421 atctgaacct cccatggcta aagtcacaag attactgatt tatagaattt cgcacactaa
    45481 aatatgtctt actaattccc cacaggctac ccccctagtt cctacgttct tacataagtt
    45541 atctattatc aaacatcaaa atttaaacaa gtttcaggtt catcttctat tcttgctatt
    45601 ttaaacttac atgcaattaa tactaaagat actattggat ttataatatt taataaagcg
    45661 tatggaccat atgttagtgc agatacacct aatgttgctg ttatatacgc cccgcatgta
    45721 ttccatggta ctaatggaga agttaaagtt ccactatctt ctaaagctct cgacaacatc
    45781 ttaggatgta tacctctctt tttatattcc ttattataca ttcttcctgg tataactata
    45841 gatagatatt gttcacctgc tattatattg gtaattgtac aagttaccat agtacataat
    45901 actgttccaa atacaccctt tgctaatctt agtaaagaag atgctactac ttcaagcata
    45961 cctgtttttt ctaaaatacc tccaagagat aaagcgcata ttgttaagga tacagtggac
    46021 atcatattca taatcccacc tttagataat aaagcatcta cctcctgtat ttgtgttgac
    46081 gaaacatatc cattttgtgc tgcatctaat atcgtattca tagattctcc ttgaaaaact
    46141 actgcaaaca atactcccaa tacagcacct actataagcc ctggtatagc aggaacttta
    46201 aatacaacca atccaattac tattgctgga gctaataata atactggtga taaagtatta
    46261 aatgtagaat ctaatgtaga cctaattaat tctatttgtt ttatatctag tgcttgacct
    46321 gaatacttca ttccaattac accataaatt actaaacaaa ttaataatga tggtatagtc
    46381 gaatatagca tatattttat atgtgtaaac aaatctgttc ctgccattgc tggagctaag
    46441 ttagtcgttt cagataaagg tgatagttta tctccaaagt aagctccaga tattatagaa
    46501 cctacgatta atccaattgg catacctaaa ctttgaccaa ttcctaaaag agctactcct
    46561 actgtaccca ttgtgctcca agatgaacct gtcgcaagag atactattgc acagactaat
    46621 gttgatgcta ctaaaaaaat atttggtgat aaaattttta gtccccagta aatcatacat
    46681 ggaactactc cacttactat ccaagttcct ataagtacgc ctattataat tatgattaag
    46741 attgcttgca ttgccatctt tatactatta aacatactta attctaactc tttccattta
    46801 aatccaagtc tatatacagc cacaagtcct gctactgctg ctgctcctat tagtggtaca
    46861 tgaggtgatg aaccatacac taatactcct agacttaaaa ataatatcaa acaagtaatt
    46921 ggtatcattg catcaaacag acttgctttt ctacattctc gctctttcat aaaatagttt
    46981 cctcctaatt atttaaacac tacactataa ttttttttga aacatatttg ctcgtactag
    47041 atgacatgat acccccataa gttaataaaa attctaaatt gtcacctaat ttataatcat
    47101 attttgtgtt acttacatct agtatcatgt ggtctgaact tgcccctaat atttttatat
    47161 cagtgtctat tggaattaaa gagtcaatat ttatatcttg tttacctata gctatgattg
    47221 ccctttttaa gattccctta tcttcataaa caggtttatt tctaaaggca tcaactccta
    47281 tttctccaat aggaactgat ggtttttctt tacattcaac tacttggcat attaatttaa
    47341 atgcatcttg atatgttcca tttatatttt caccgtatgc cgtttctcta ccaagtagaa
    47401 tggactcgcc tattctcaaa ttagtaatac cttctggcat attattattt atcaacagat
    47461 gaatagaact tgaattacct ccagatacta tttctagatt tattccaaat ttttcttcca
    47521 ttctttttgc tatacttacc agtctgccta tattttccct agatggtata atagctccat
    47581 aacaggttaa gtttgtagct ataccaatta tttcaatatt ttttaatctt actatctctc
    47641 ttacattatt aaaaaagtct tcttcataaa agtatccttc tcgtaaatcg cctaagtcaa
    47701 acataagaac tatcttatga ataatatttt tactttcaca taattcattt aatttcttta
    47761 ttgttcctaa ttctgagtta aaactaatat cgcaataatt gataacttct tctaattcac
    47821 ttaacattgg gattctaatc aatatcttag gtagatttat atcttgtaaa ttttttaagt
    47881 tttgtattct tgaatctgct atatggtcta ttccttcact acatatttct tctactattt
    47941 ctttttgtgc acaaaatgat ttagtaacaa aagatatctt aatatttctt tcatgacaca
    48001 tctgcgaaat taattttgca ttatgcttta acttttctat atctatttct aacctaggat
    48061 acataatctt tcctcctcaa aattcaaact ttttaacagg agcttaagag atgcttcttc
    48121 atatttttta gataaaacac ccatagatgc cataatatag tcattatcta ttaatatctt
    48181 tgttttttcc tttggaaata gtaattcatt atttttattg tatttgagca tattatctaa
    48241 tatttcaatt ctactaggga gcctagtaag tgctccccct gtaccaacta tatatcttac
    48301 ttctgttaaa tctttccctt ctgcaatttt gacttttcca gttgttccat agatatttct
    48361 tatttttcct gaatgtctta gcatagcttt aacaacagct tcttttgtca atctttcaac
    48421 aaataatatt tcttcttttg attttggtat tggaggataa ttctctatta ctgcatcaat
    48481 atctatagat agttcacttt gtaagttttc tttacctata acttctacta tattcttcat
    48541 atttacatat acacctaaat ccccctcaac acttctcttt gcagtaggtt caggattaac
    48601 caaaatttta ttaatatagt cacttctatc tgtaacagaa tgtacatcag tagttgctcc
    48661 acctacatcc agagttaata aatctccaat atttttatat aaaagtttag aagcttccat
    48721 tacagctcct ggggttggag taatattttc attaaccatt tctttaatgt atttcattcc
    48781 tggtgctgta gttatgtgtt cttcaaatac actttgaatg attcttcttg taggttctat
    48841 attcaataaa tctattttag ggtatacatt ttcagttata taaagcttat aattagtatc
    48901 ttcaaatatt aaccttattt cctcttgatt ttcaacatta ccagcatata taactggtat
    48961 gcctagattc atagaagcta tcatttctgc attataaata gctgtatctc tttctccata
    49021 atcgactcca ccagctatta aaattatgtt tgggtttatt tcttttattt tatttaaatc
    49081 agttctttta attctacctg atgttatttg acgaataaca gctccagccc ctaaagctgc
    49141 ttctttagct gcttttactg tcatatcata gactaatcca tgaacagtca tttttaatcc
    49201 accagctgca ctagaagttg caaacatatc attatactct atatcatcta cagataattt
    49261 attagctaaa tctttaatag cacctgataa gccatttctt acatcgccac cttcaaacac
    49321 tgttgttgga gcttgacctt gacccaaaaa tataggattg ttagtattaa tatcgtggaa
    49381 agcgtttacc actgtagtag tacttccaat ttcagctacc aacacatcta tttttaaaga
    49441 aaccttgcta ttttccatta tttattactc atttctcttc ttttttcaac taagaaagtg
    49501 gctacatgta tgcctttact gtttcttcca aatccagcat ctatgccttg ttcaacagct
    49561 atttcaggtg ttacttgtgt tcctccacac gcaatcatta ctttatctct tatacctttt
    49621 tctacacaat aatcgtgtaa ttttttcatg tttttatagt gaatatcatc atgacttatt
    49681 attgtagatg caagtattgc atctgcattc agttcaatcg ctgcatctac caatttttct
    49741 acaggtacag aagtccctag atagtggcat tctatgccat atttttcaat acctccatgt
    49801 tttatatcta ttatttctct taaaccaact gaatgttcat cttctccaac cgtagctgaa
    49861 actattttca taggtttttc ttctatatct gctcttattt cttcatcagt tagtacctta
    49921 ggttcttctg gtattactaa attgtttaaa tctatatcaa acggaactct tcctttcact
    49981 tctattctag tcccttcaga ttcatgcata acttctctac ttatgacctc aatttcttgt
    50041 aagttcattt ttttgacaaa ttccaaagct gcaaactctg ctgttctttt atcagtagga
    50101 agcatcattg ttatcattat aattccatct gcacaccatt ccatttcagg cttaatttta
    50161 gttccatttc tgtactcata tgattctttt agtctatttt ctacattatc aaaatcgtca
    50221 agctcatcaa tatatattat tttgtctgga ttttcaaatg tacatccatc tattaacatt
    50281 gatggattat ctatagcatt ttcatcatat tgagctacat tattgtatcc aaagtgagca
    50341 gttacaggtg ctaaataatc ttcttctctt tcataaacag taccttcacc aactccacct
    50401 tttatttttc tagatattcc atcaccattt ctttctggat aatttccaga atcaacaaaa
    50461 aatccttgtt ccacagattc aaaatatcca ccaacttcag atatttcttc catatataat
    50521 acagctcttt ctttaagttc tcttgctttg tctcttaatt caccgacatc tttaagttct
    50581 atcatatcca taagcccatc cataccaact aaagcttgct ttgctgtatc acatgcttct
    50641 atattgtata tatgccaagg tacatttcta ccttcatcag gtgttatagt tgattgtatg
    50701 tcagctctag ttaatactga tgttaataag tttaatacat gagtaactgt tgcttctcta
    50761 gttgatgatt ccatatattt agtattcatt tgagctctca tcttataatc actaaataac
    50821 tctctaagtg caacagcgta aggtaagtct atttttaaac atggtgctgg aggtgctgtt
    50881 ggtggaactg ttgataagca tatattactt ttatctatac ctattttttc tgagaatata
    50941 gagtttaaag cgtgttgaac cataagttca ggcattacct tccatgcttc tctagcagta
    51001 gcattagcat tatgtgctcc atcaatttga gctatattgg caaaagccat tattttcttt
    51061 gcctcacaag catctacaaa agaccttacc atgtttatat tcctatacag aacattatat
    51121 tgtgggtctt gatgtgctcc attaactcct tcctcagcaa acattactgc tacttcagga
    51181 ccagcaacac cacttacata tgaatgataa ttaataggtc taccaacttc ttcttctata
    51241 aaatcaagag ccttcctttg agctcttact tgttttcttg ttattggaac tccacctata
    51301 ccttgtgggg ttccttctat aagcccatca aaatgtgatt gtccagcagt tctgataacc
    51361 attagatggt cagctccatg atgagctgcc attctcattc ttcttatatc atcctcaaat
    51421 ctaccagaag ctatttcagt agtaataact ggacttggtt gagggtctat attattgaaa
    51481 tacttagatg atggtagccc tacactcttc tttaaaggtt ttgtacagtc tttatattta
    51541 aaaggtccca tttcaagatt ttccaagtgt tctctccaaa cccaacctct tcgttttggt
    51601 ttatatttat ccaaatcttt taatatatag tctatatcca attttttatt ttcctttaac
    51661 atgattctac cccttctcta aataaacatg taacttcatc ccaatattca cccttaacta
    51721 ataacaaacc tgcttctcta accgttatat ttttagattt agccaattta taaactacat
    51781 gccctgctcc tttacctata agacctcttt ccataacccc ttctaaaata ggttttactt
    51841 caagagaaga aaatcccatt ctcaacaaga cagacctttc tattgatggt gttgtattct
    51901 ttttaccaag ttctatcatt ggttctacta tctgggtagc taaactccaa aacctttctt
    51961 ttaactcatc atcactcagt tcttgaagat gttttcttct aacttcaaaa tcatcctctc
    52021 tttttttcat gaacattcct ccattcgtat tgatttaatc gaattttctg aatttttatt
    52081 ttatattaaa ttaaaaaaca ataattacta taaattattt aatacatcaa caacaaaatc
    52141 tcttgaactt tttacttctt ccattaaaaa ttctatatct cgattactta gcttatcaat
    52201 attatcaatg tgattattag atatacaatt ttttataaat gactttctta ttttattcat
    52261 atcaatatca acacatttca ataattctgg tctttctggt aatattaagt ttactcctgc
    52321 aatttcttct cttggatttc caaattttat atctattcca ttttctcttg caaaggtaag
    52381 ttgtggacat atatgtttac ctgcacccgt atattctgtt tcttgtacca ctatagcttt
    52441 atcctcatct agctcttgag ctaaagagaa tgctgctgct aaagatgtat ttcctgctgg
    52501 acctctttct ataccttcta gctgtgccaa taattcagtt gtataaaaaa cttctccttg
    52561 ttttactaat acatatctat ccatatatct aagtggtcta gctgcagacc taggtacatc
    52621 tgacctatca ggccatgtac agtatggtat cccaaaacct gtatgtcctg ttgtaaatga
    52681 ttttttatta aattgattat cactagccat gtgtaatcct tttaaatcta cacttgctcc
    52741 tactacttta gtattaatag aaccagcttt tatcaatcct cttgcagttc cagtaagatt
    52801 accaccacct gcatttgtac aaacaactat atcagggtct tttttatact tttctctaaa
    52861 ttggatagct aactcataac ctaaagtttc tacacctgca attccaaatg gtgaatataa
    52921 tgatgcgtta aaatagcctg tttcttctaa taaaactaag aacgtataaa ataactctgg
    52981 tccaacagtt aactgaacaa cttctgctcc atatgcctca cattttcgcg ctttttctat
    53041 gatttcaggt tgacctacac cattagaatc ataacattct tgaactatta tacattttaa
    53101 tccctgcatt gccgcttgtg atgctacagc tgcaccatag ttaccactgg tagctgctat
    53161 tacaccttta tatcccattt gtttggcatg atatactgca gtagctgctc ttcttgcttt
    53221 aaaactacca gatgcattca ttgcttcatc ttttacaaat attctagctc ctttaccttc
    53281 tttagcacat tttctagcta atttagtcaa atttttaagc tctattaaag gagtatttcc
    53341 aacagcatac tgtgattgaa ttgcttcaat atcttgtaat gtatatcctg tttcactcat
    53401 cattttctca taatcaaacc ctattccctc ttgttcaaaa gaagaataat ccaatccgat
    53461 tgcatttttc attatttcgt tatttcttcc cataacagct tgataactca tatctttcat
    53521 tgatgttgaa ttattcatta tatacacctc caaataatat ttctttagct tggattccta
    53581 tttggagtaa ctctggtata catttaccat aatcatgagt ataatatgga tttattttaa
    53641 gtaaattacc ttttacaagt ctgcctgtaa ttgttttaac ttctactaag tcgcctatag
    53701 aagcatcact ttgtataaaa cctttaaccc acatttctaa ggatactttc ttagtatcct
    53761 ctggaacttg tggagctctt tcttctggag tcaatactat attgtgtata ataacccaat
    53821 cattaatttt agcatccatt ttttcatccc cttattaatt gactattgcc ttactaatta
    53881 atttactgtt attttaaatt aaacattatt ttaccaatta gctaactgtt atcttactaa
    53941 ttagctagtt gctatcttac taattagcct ttgattcttc ttccaattca acttctatct
    54001 gatttcttat atctcccatt attgctctag gtactggtat atccaacata gttttaagac
    54061 ctggtctagc atttattatt aatggaatag agtttactat cattgcataa gtacctactc
    54121 ctccaggtat ttcaggattt atttgtaaat caatgtttgg tatacctttt attgatacat
    54181 agtctcctgt tttaatacct tctgcttctg gtattatctg ttgtggatgc tccatttcaa
    54241 ttaaaacttc tccatttaca tatccatatc cacattgtct acaaccagca acatttccag
    54301 ccaatgcctc tccgtactta gattttctat ctactgtagt cattattggc tcttttgttt
    54361 gttctatctt atctaatttc caacctaatc catcagctat catatttatt gattctacaa
    54421 aacctacatg acctgctata gtcttatctt ctacaccttt tataaattct tcccttgtta
    54481 ctccaacacc ttgttccacc ataacagact ttccaaatgg agataaatca tttactctct
    54541 tagcttttat actagtaact tcttcacaag tacctgaaag tgccaatact agtaaatcta
    54601 atacaaatcc tggatttatt cctgttccaa gtatacttac tccattttcc tttgcaactt
    54661 catccatttt tttagctaaa tctgcatcat cagcttgtgg atatgccatt tgttctgctg
    54721 ttgatataac atttattttt ctatttaata agaaatttat tttatcaaaa gcctttttag
    54781 taaatgaatc agttgctaac aaaacaacat ctacactttt ttctctaaaa acttcttcat
    54841 aatttttatt gataataact tctggtcttt ctcctctttc aattcctaaa acatcataca
    54901 tacttttccc tgaagtactt ctactacaaa cagatactat ttctatacct tctttcttta
    54961 gaatcatatt agccatacct attcccatag ctccaaatcc ccatattcct actcttactt
    55021 ttctcatata tacaccctcc taaaaattta tatattctca ataattagtt aaagcataaa
    55081 tcgtgccaac tttaaaatta aagtaaatat ttattggaac gttgaaacta taattatttt
    55141 attatgtatt ttcataaata aaaaataata tttcttatga gaaataaaaa aaagagcaaa
    55201 atattttgct cttttataat aattttgctc ctctattttg cttttttatt ttactttgat
    55261 tttgtttttt tatcttgctt atttataata ttttgatatt atattttttt attttatact
    55321 gtaaattttg cctattcata cctaaaattg atgcagcttt agttatattg taagatgcct
    55381 tttctaatga ttcatatata tacttttctt ctatctcact gataatctta ctaagtgttt
    55441 tgtcctccaa atcgtttaaa gaataattgt tactctcttt attctcttta agagtgtaat
    55501 agtcgtttag attaatcata cttgtttcta tttgtacctc atcttccatc atactcatag
    55561 tacaataaat tatgttttct agctctctta tgttaccttg ccaatcgtaa ttttttaaaa
    55621 ttttaatagc ttcatctgaa attcctacca cttttttatt taacttttta ttatactttg
    55681 aaataaattt tttgcacaat agaggtatgt cctcttttct atctctaaga ggaggaattt
    55741 ttatatgaat tatgttaagc ctataatata aatcttttcg cattttatgc tcgtttaaaa
    55801 ttacctttgg actttcattt gtcgttgcaa taattcttac atctataggt atatccttag
    55861 ttcctccaac ccttcttaca taaccttctt gtaaaaccct taatagttta gcctgtaaca
    55921 taataggcat ggaatttatc tcatccaata acaatgtccc tttgttagct tgttcaaata
    55981 agcctggcct atctattgct cctgtaaatc ctcccttgct agttccaaaa agaatacctt
    56041 cgaatagtga ttccggcaaa gctgcacaat tttgtgctat aaatggatag tttctcctat
    56101 tacttccata gtgaatggac tggctaaata actctttacc cgttccagtt tcaccgtata
    56161 taaatactga agcatcagac ttgcaagctt tcttagattt agctattgct tctttaatca
    56221 aattactttc ccctaaaata tcatcaaatt cataaccctt tttttgtcta atactaactt
    56281 tattgactaa atttttttta taatttctat ttagtaaatt ttctatcgtt gtcatattct
    56341 ttgatatttc aagtgcccct acaaactcgc ctttaatatc aattggtatt gtagtattaa
    56401 ttgctgttat ctcttttcca tttttatcca gatatttttg aatattgtct ttgataactt
    56461 cccttttttc caaaacttct aatagtgtac tcctgtttat tttcattttg ttgactatat
    56521 caaaaaatga attggttaaa atatcgtgtc tgttcatttt ttccattttt tgtacagaat
    56581 tattgtagat tatagttttc ccgtctttat taacaaaatg taccccttca tcaatatttt
    56641 ccaatatttc attcattatt ttaaaaaata tctcttggtt caactatatc accccagtac
    56701 ctaaatatat aaaaacccaa aaaattaata aaatgttgct attgtattta tcttatcata
    56761 tatttatgat attttttatg taca
//
LOCUS       DAMJVI010000011        56080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.011, whole genome shotgun sequence.
ACCESSION   DAMJVI010000011 DAMJVI010000000
VERSION     DAMJVI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 56080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.011"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            789..2459
                     /locus_tag="PC545_000774"
     CDS             789..2459
                     /locus_tag="PC545_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF and EAL domain-containing protein"
                     /protein_id="HDF2455564.1"
                     /translation="MREESNSLSLDHIEIFFELLSQSTEDYIFFWDINRNKFKISSAI
                     FDEFNLSKEIESDLVNCWSKIVYPDDVQIWKDDIQELLHGKKGEHNLEYRLINKYKEI
                     VWISCRGKVYVSDDPKTIFLVGRIKNIGEKNKFDSITGTWNREQFEHKMNYLIKEKIY
                     KNGAMFIMDIDNFKNINEKYGHSYGDKVLRAIATEVLEYLPKDVRLYRLDGDEFAFFY
                     PMCTKETIEKIYEKIQMYTNTQHEIESSKYYCTVTAGVAMYPEDGDNYLDLFKHADIA
                     LDIAKISGKNRIKFFSQELYENKLKVISMQQKLRECVENNFNDFELFFQPQVNAVTKE
                     VIGAEVLLRWHSSTYGEVSPVEFIPILEQSNLIIPVGKWIIKEAVKQCKEWHKINPDF
                     KISVNVSYIQLKEDFFRDFIVECLVEYQLRPEFLILELTENCWIPDINLLNDKFISLK
                     GIGVYIAIDDFGTGYSSLNYLKELSVNIIKIERSFVKNITYNSYEYTFLEYIIKLAHI
                     INLKVCVEGIESYEEYDIVKSLGVDIIQGFLFGRPVSASEFYKFQLSK"
     gene            complement(2581..3315)
                     /gene="pflA"
                     /locus_tag="PC545_000775"
     CDS             complement(2581..3315)
                     /gene="pflA"
                     /locus_tag="PC545_000775"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDF2455565.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSGGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDIDNLEKLGQFVATLKNVERFELLPYHSMGIHKWESLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(3475..5706)
                     /gene="pflB"
                     /locus_tag="PC545_000776"
     CDS             complement(3475..5706)
                     /gene="pflB"
                     /locus_tag="PC545_000776"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDF2455566.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDEAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            5999..7060
                     /locus_tag="PC545_000777"
     CDS             5999..7060
                     /locus_tag="PC545_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDF2455567.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDRLFITVVLFLVGFLLITKGADIF
                     INCTVEIGKKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFISPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKNRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVIIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAVSAIPNNIPILSQNRQLDIPFMILLLLIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            7151..8764
                     /locus_tag="PC545_000778"
     CDS             7151..8764
                     /locus_tag="PC545_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2455568.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLEGFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
     gene            9039..11024
                     /locus_tag="PC545_000779"
     CDS             9039..11024
                     /locus_tag="PC545_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2455569.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSNVLGVSTRTIRNYIN
                     QINRELKGGIAKIVKNEKGTYSIRIEDESKLIEIVNFNRGKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLILDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIMSVLRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIENAILLKNSINLKSIK
                     KIEKNIPFIGKLIEEDKFFILNKSTFMENLEDMLEKLVHKGIIDIEFKKKVLKREKKS
                     STALDNYIALPHSVNTNGENLYLAFGILEKPVIWDTKEIRIIILMIIPDKNVDSTDLI
                     IKSYEEVLEVGRDKKMVEELAKVKSFDEFYKIITKRR"
     gene            11029..11463
                     /locus_tag="PC545_000780"
     CDS             11029..11463
                     /locus_tag="PC545_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDF2455570.1"
                     /translation="MDPVIFIIASLITVYLLNIFLGYLQLKDFNKNYIELKRKGRVAI
                     GRKKGRISSGTIVLILINDDGVIVETRKMQGVTVLARVKSFEGLVGKSLEYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDTNNLSTDEECRMEVV"
     gene            11492..12052
                     /gene="srlA"
                     /locus_tag="PC545_000781"
     CDS             11492..12052
                     /gene="srlA"
                     /locus_tag="PC545_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2455571.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            12116..12484
                     /locus_tag="PC545_000782"
     CDS             12116..12484
                     /locus_tag="PC545_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HDF2455572.1"
                     /translation="MAFNKVKIVKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            12509..13075
                     /locus_tag="PC545_000783"
     CDS             12509..13075
                     /locus_tag="PC545_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2455573.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            13116..13481
                     /locus_tag="PC545_000784"
     CDS             13116..13481
                     /locus_tag="PC545_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2455574.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTDDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            13582..14355
                     /gene="srlD"
                     /locus_tag="PC545_000785"
     CDS             13582..14355
                     /gene="srlD"
                     /locus_tag="PC545_000785"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDF2455575.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAE
                     EIKTEFGCKSVGYSCNAIDERQVISTVKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFIDVANMLTFYASKEAEYCTGQSINITGGQVM"
     gene            14704..17142
                     /locus_tag="PC545_000786"
     CDS             14704..17142
                     /locus_tag="PC545_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2455576.1"
                     /translation="MDIIKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDGVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGGPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYVQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKKYLSSLELSFLIDECGVKENDIREL
                     VDLYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSQSYGEDREFYVSNISKFIEESLKNTSNKVNYASDELDMALHKVNYISDELD
                     TTSNKVNYVSDELDMALHKVNYVSDELDTTSNKVNYVSDELDMALHKVNGVSDELDMA
                     LHKVNCVSDELDMTSNKVNYVSDELDMTSNKVNYVLDELDITSNKTDYISNELYNLSN
                     KSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFITDTVTLCDMFIP
                     CTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMGLNDYPFVEKR
                     TYLEKVIEPLKRFDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESIKNLG
                     ISPTPVYISNKYKEIEDKNISFRLLTNHHADTLFSQHFMDKKSIAQAYINQRMAKKVG
                     IEEKDIVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNSLTDTGISDFGGQ
                     VTYNESMVEIIRQN"
     gene            17230..17565
                     /gene="spoIIAA"
                     /locus_tag="PC545_000787"
     CDS             17230..17565
                     /gene="spoIIAA"
                     /locus_tag="PC545_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HDF2455577.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            17579..18007
                     /locus_tag="PC545_000788"
     CDS             17579..18007
                     /locus_tag="PC545_000788"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HDF2455578.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            18030..18794
                     /gene="sigF"
                     /locus_tag="PC545_000789"
     CDS             18030..18794
                     /gene="sigF"
                     /locus_tag="PC545_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HDF2455579.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            19074..19511
                     /gene="spoVAC"
                     /locus_tag="PC545_000790"
     CDS             19074..19511
                     /gene="spoVAC"
                     /locus_tag="PC545_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HDF2455580.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            19678..20691
                     /gene="spoVAD"
                     /locus_tag="PC545_000791"
     CDS             19678..20691
                     /gene="spoVAD"
                     /locus_tag="PC545_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HDF2455581.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLSDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGDGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGVDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            20705..21067
                     /gene="spoVAE"
                     /locus_tag="PC545_000792"
     CDS             20705..21067
                     /gene="spoVAE"
                     /locus_tag="PC545_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HDF2455582.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            21250..22266
                     /locus_tag="PC545_000793"
     CDS             21250..22266
                     /locus_tag="PC545_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2455583.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAIFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            22641..23483
                     /locus_tag="PC545_000794"
     CDS             22641..23483
                     /locus_tag="PC545_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2455584.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTRRT
                     SNKL"
     gene            23498..23962
                     /locus_tag="PC545_000795"
     CDS             23498..23962
                     /locus_tag="PC545_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455585.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLRLNGML"
     gene            23949..25121
                     /locus_tag="PC545_000796"
     CDS             23949..25121
                     /locus_tag="PC545_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2455586.1"
                     /translation="MECFKEVVNMNELKAKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFTGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTNVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            25165..26100
                     /locus_tag="PC545_000797"
     CDS             25165..26100
                     /locus_tag="PC545_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HDF2455587.1"
                     /translation="MILKQVIEVYDILDKANANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKLKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(26374..29067)
                     /locus_tag="PC545_000798"
     CDS             complement(26374..29067)
                     /locus_tag="PC545_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2455588.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWELISNDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLELKYIKQSEYDEAIKAKIEIKLPQSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDRNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQAT
                     ALSDSRGGYKFKENNEWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANHG
                     IYVEPSIFTTITTFDGQLLVKNAPEEHKVVDPEVAYVVTDMLESVITEGTGGVATLPK
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKIMEKIHANLTVTEFEVPKNVYFTKINLEDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQPEENNNNNNNTGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNNGGGSTTPPDGGGTPAT"
     gene            29281..29979
                     /gene="yunB"
                     /locus_tag="PC545_000799"
     CDS             29281..29979
                     /gene="yunB"
                     /locus_tag="PC545_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HDF2455589.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(30195..31208)
                     /locus_tag="PC545_000800"
     CDS             complement(30195..31208)
                     /locus_tag="PC545_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HDF2455590.1"
                     /translation="MQLKNFHFKNNTLSKKTNLAKSKFTIVLTFLFLFLLYFFSNNLI
                     YAQNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYS
                     DFANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTS
                     EFGAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENT
                     DFGIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNKCKKYNIEIIDIENQKH
                     PESKSFKIKITDKKLLAQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIK
                     WML"
     gene            31436..32320
                     /locus_tag="PC545_000801"
     CDS             31436..32320
                     /locus_tag="PC545_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2455591.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ANKDENSNKNNKDKNEDKSNSKSNKDKSSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKSILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIVQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDAYFK"
     gene            32522..34072
                     /locus_tag="PC545_000802"
     CDS             32522..34072
                     /locus_tag="PC545_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2455592.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQEYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            34101..34748
                     /locus_tag="PC545_000803"
     CDS             34101..34748
                     /locus_tag="PC545_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDF2455593.1"
                     /translation="MKKFLNVLFSICISIVIIVGVINFTVGFKQLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFKLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLVVSLVGIILSVKNKNIKILKTTSITLIIMPLLLTVPILLNFEKSFIIFHKLLFRND
                     YWIFNPDLDPVINILPEEFFFHSGMMILILILLVSIVLFVMYKLCKSLSIKIFQC"
     gene            complement(34890..35150)
                     /locus_tag="PC545_000804"
     CDS             complement(34890..35150)
                     /locus_tag="PC545_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HDF2455594.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCILPHKKDNYNYIMSKFQDLVKITSKK"
     gene            35367..36650
                     /gene="hflX"
                     /locus_tag="PC545_000805"
     CDS             35367..36650
                     /gene="hflX"
                     /locus_tag="PC545_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2455595.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTNESMEELKELTKAAGAE
                     VVGSLIQNKHSVDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGESGITLDVDLDEEDFNIYREYILEK"
     gene            36658..37335
                     /locus_tag="PC545_000806"
     CDS             36658..37335
                     /locus_tag="PC545_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HDF2455596.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDENSLKILQQLKDKNKNFEKEYVLRAYDKAIKSLE
                     NAKNLKK"
     gene            37421..37864
                     /locus_tag="PC545_000807"
     CDS             37421..37864
                     /locus_tag="PC545_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2455597.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVLAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            38169..38813
                     /locus_tag="PC545_000808"
     CDS             38169..38813
                     /locus_tag="PC545_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDF2455598.1"
                     /translation="MSNYRTLHEFGMDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVAVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLEAGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTVYEDNVEIVVYSRLEDVEKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILI"
     gene            complement(38899..39276)
                     /locus_tag="PC545_000809"
     CDS             complement(38899..39276)
                     /locus_tag="PC545_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2455599.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGIF
                     LLLVGLFEVKKGFCAKSDKKPLN"
     gene            complement(39276..39662)
                     /locus_tag="PC545_000810"
     CDS             complement(39276..39662)
                     /locus_tag="PC545_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDF2455600.1"
                     /translation="MKKTDDLKKKYFDFNLKNTIIGVFTGFINGVFGSGGGTLLVPIL
                     NDIVKVEEHKSHATALSIIIFLTTASSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKL
                     LNKVTGKFLRISFGIIMIIAALRMVF"
     gene            39841..40596
                     /gene="nadE"
                     /locus_tag="PC545_000811"
     CDS             39841..40596
                     /gene="nadE"
                     /locus_tag="PC545_000811"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HDF2455601.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPAQPPKF"
     gene            40679..41398
                     /locus_tag="PC545_000812"
     CDS             40679..41398
                     /locus_tag="PC545_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2455602.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKEGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            complement(41565..42569)
                     /locus_tag="PC545_000813"
     CDS             complement(41565..42569)
                     /locus_tag="PC545_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2455603.1"
                     /translation="MATIKDIAKDCGLSSATVSYVLSGKGNERRIPLHTQELILNSAD
                     KLGYKRSTPIKHNNIKIAFYWQDEGFELASTNILQAINHIISLEHLNINIIIRPYKVG
                     HLCDDEYFFANNYYDGIVLTGINADDIEYLVKNKPKIPVVVVNREIPGFSSVSIDNEE
                     TGRIAALHAIQNGKNDIAVVFNPTSFTCMTLRGKSFLKTCKEHGIHLSDSIYYCSNKI
                     DSGYDIAQKMLAKNSLKKVIFCNHDVVALGMMNAFHESGIVIGEDIQIFAASNGPSRL
                     MARSIPPLTVIDLKMEETAERSLRMIIDTVTGRINGIQQQVIYPSIIYRQSSPAQNLL
                     "
     gene            42835..43731
                     /locus_tag="PC545_000814"
     CDS             42835..43731
                     /locus_tag="PC545_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HDF2455604.1"
                     /translation="MSKVNIFEVGPRDGFQNLKEFLETDKKLKIIDGLIASGIKQLEI
                     SSFVSPKAIPQMKDAKEVATYCVEKYPDVKLYALVPNLYGAKVAWECGIRDISYVISL
                     SETHNKKNINRTHEESIDELKKIMNTYPDMNICIGMATVFGCPFEGLPKIEDAIRFAK
                     RLWENGIREMNLADTIGIADPKQVRETISALVEALPECRWQVHIHDTRNMGMVNTLVA
                     IESGITTVQSTLGGLGGCPFAPGASGNTATEDLVYMLERMGYDTGVDFVKLLELSKYQ
                     KSFVNGVYSGHHVNIKSDSCNC"
     gene            43869..45131
                     /locus_tag="PC545_000815"
     CDS             43869..45131
                     /locus_tag="PC545_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455605.1"
                     /translation="MDIIALLLFLGVIIIAFVRKNNAGILALAIGVIAVRIFGMDDKE
                     LYGAISNSMFTTLVGITLLFAIVNSTGALKLVAEKIVAASGKRVWLIPIAVFIAGFVV
                     AGVGPGAIPALAIIPAMAVPVAITVGYNPVMLALIGECGLMAGRMTPITPEGQIITTV
                     AEGVGIENVMPTILACQTLSTIVFSIVLFIIFKGYKLKKPINVIDSKQIEKFNIKQII
                     SLISIVVMIAFIIIFDMNVGLAAFAVSAVLIFFNIADEGECIKSIPWSTIVMVLGVGA
                     MMTIVDAAGGIDLMSNALSSLMNSKTATPIMSISAGLMSMVSSALAVVYPTMMPMCVD
                     IAKAVGGVDPLALIAAVGVGGSLAGVSPLSTGGALILAAMGSSKKDFNKEEQNKVFVQ
                     LFIMSAVGLLVIAVVSILSFNAITHLMN"
     gene            45207..46400
                     /locus_tag="PC545_000816"
     CDS             45207..46400
                     /locus_tag="PC545_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2455606.1"
                     /translation="MKKGALSNIKVLDLTRVLAGPYCTMMLADFGADVIKIEMPRKGD
                     DTRGFGPFVNGESLYYANINRNKKGITLNLKSEEGKNIFLEMVKHADVVVENYRPGVM
                     EKLGLGYDELAKVNNQIIYGAVSGFGCYGPYSERPGYDIIAQAMGGLMSITGQEGGEP
                     TRVGNAMGDVLGGMNLTIGILTALNARKIIGKGQRVDVSLVDSVVSSLETGIQRYFDS
                     GQPELMGNRYASAYPYDSFKASDGSFVIGCGNDKLFEKLCKKVLFREDLLIDERFKTN
                     LNRCEHYKALKPEIEKWSIKHTIKEAVDLISEAGVPAAPIYNLDCVTTDEHIAKAREM
                     IVDISHPIIGNMKVNGNPVKLMTTKAKIDMPAPSLGESNEEIYNGWLGFDSDKIKDLK
                     ENGVI"
     gene            47029..47823
                     /locus_tag="PC545_000817"
     CDS             47029..47823
                     /locus_tag="PC545_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2455607.1"
                     /translation="MESVMENLNNLKVEVKDKVCVITINRPKALNALNSDTLRELSQV
                     IDVVSENEAILGVIISGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGVFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESMKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKNALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            47918..49258
                     /locus_tag="PC545_000818"
     CDS             47918..49258
                     /locus_tag="PC545_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2455608.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFIVGSTILYKMVSKEKANGGHFIA
                     HESDIFDEAEATNSKRENGPNGVIALIPLIVSILIINVKVNGKAIVPIEVGVFIGALL
                     VYLLLNKYQDNTKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            49395..50627
                     /locus_tag="PC545_000819"
     CDS             49395..50627
                     /locus_tag="PC545_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HDF2455609.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSETGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKDGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            50667..51809
                     /locus_tag="PC545_000820"
     CDS             50667..51809
                     /locus_tag="PC545_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2455610.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASRTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            51820..52614
                     /locus_tag="PC545_000821"
     CDS             51820..52614
                     /locus_tag="PC545_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2455611.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKITVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            52625..53647
                     /locus_tag="PC545_000822"
     CDS             52625..53647
                     /locus_tag="PC545_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2455612.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSICDYGIVGDLYEIIPEM
                     IESLNR"
     gene            53746..55122
                     /locus_tag="PC545_000823"
     CDS             53746..55122
                     /locus_tag="PC545_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2455613.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNRSGVIIDINDKYCDFLGKE
                     RKDIIGQNIQSIIPNTKMLDVMKNKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENRDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(55454..55798)
                     /locus_tag="PC545_000824"
     CDS             complement(55454..55798)
                     /locus_tag="PC545_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455614.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIERFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
BASE COUNT        21238 a         6628 c        10051 g        18163 t
ORIGIN      
        1 cattgacatt ctaattttgt agtgtaatcg tattttttat taattcttac aaaatcaatg
       61 aaaataagta tatccatttt aaagtacata aatttaaaaa tccagaaaaa tatacttaaa
      121 ttatgtaagt aaaagagggg ttgaatcagt tgaaaatgta atattagcaa atagtacatc
      181 aatcctatat accctgtaag caactctatt ttcatgtact aaaaatgtac ctattttatt
      241 aactgaaaag aataaattaa atgtaagtac ataagaagat agaaaaaatt aaaaacaaaa
      301 aatatttata taaataaata taaaaaatga tataatccct attaaaaaat ttatagatga
      361 tgaagaaata tcaaaatctt atattatagg aagagacact tctttgccaa aatctataga
      421 aaaacaatac caaatccaaa gagaataggc ggagaaaaaa ataaaataaa ttcaagagta
      481 atagaaaagt tttatagaat aaagaactga taactcttta tataacgaaa aatagaattt
      541 taagagaaaa tgatttaata actgatttaa catggtgttt tagcaggtaa aaataaatca
      601 ccagtaatga ttatagaaaa attactttat gaatctcaaa aaacttagtt aagagtaaga
      661 gatttgataa gataactaaa gttggggaaa tgtgtttgat gagacaaaaa attagtaaaa
      721 tagtataatc taaataagga tattttgttg attaaactac actaatagta atattgtagg
      781 gggaaaagat gagagaagaa tcgaactctt taagtttaga ccatatagaa attttctttg
      841 aacttttatc tcaaagtaca gaagattata tatttttttg ggatataaat aggaataaat
      901 ttaaaatttc ttcagcaatt tttgatgaat ttaacttaag taaagagata gaatctgatt
      961 tggtcaattg ttggagtaaa attgtttatc cagatgatgt tcaaatatgg aaagatgata
     1021 tacaagaatt actacatgga aaaaaaggtg agcacaattt agaatatagg ttgattaata
     1081 aatataaaga aatagtatgg atttcttgta gaggtaaagt ttatgtgtcg gatgacccaa
     1141 aaactatatt tctagtcggg agaataaaga atattggtga aaaaaataaa tttgattcta
     1201 tcacaggaac gtggaataga gaacaatttg aacataaaat gaattatctt ataaaagaga
     1261 aaatttataa aaatggtgct atgtttatta tggatattga taattttaaa aatataaatg
     1321 aaaaatatgg acattcttat ggtgacaaag tgttgcgtgc aattgctact gaagtactgg
     1381 aatatcttcc aaaagatgta agattgtata ggttagatgg agatgaattt gcattttttt
     1441 accctatgtg tactaaagaa actatagaga aaatatatga aaagatacag atgtatacaa
     1501 atactcagca tgaaattgaa tcaagtaagt actattgcac agtgactgca ggcgttgcta
     1561 tgtatccaga agatggagat aattatttag atttatttaa acatgctgat attgcattgg
     1621 atatagcaaa aattagtggt aaaaatagaa ttaagttttt ttcacaagag ctctatgaaa
     1681 ataaactaaa ggtaatttca atgcaacaaa aactaagaga gtgcgttgaa aataatttta
     1741 atgactttga gctgtttttt caaccccagg taaatgctgt tacaaaagag gtaatagggg
     1801 cagaggtact tttaagatgg cattcgagta cttatggaga agtatctcct gttgaattta
     1861 taccaatact agaacagagt aatttaatta taccagttgg gaagtggatt ataaaagaag
     1921 ctgtaaaaca gtgtaaagaa tggcataaaa taaatccaga ctttaaaata tctgtaaatg
     1981 tatcgtatat tcagttaaag gaagattttt ttagagattt tatagtagaa tgtttagtag
     2041 agtatcaatt aagacctgaa tttttaattt tggaattaac agagaattgt tggataccag
     2101 atataaattt actaaatgat aaatttatta gtttaaaggg tataggagta tatattgcaa
     2161 tagatgactt tggtacagga tattcatctt taaattacct taaagaactg tctgttaata
     2221 taataaaaat tgaaagaagt tttgttaaga atattacata taatagttat gaatatactt
     2281 ttcttgaata tattattaaa ttagctcata ttataaattt aaaagtttgt gttgagggaa
     2341 tagagtctta tgaagagtat gatatagtaa aaagcttagg ggtagatatt attcagggtt
     2401 ttttatttgg aagacctgta agtgcctctg aattttataa atttcagcta tcaaagtgat
     2461 gtatataaaa tgagatatat agtatataaa aatagggcaa agtggaagtt ttgaagttta
     2521 ttaaaaattt acactttacc ctattttgtt atgtcttcta taaaggatta atatactaca
     2581 ctaactatta tatactttca ctccaaattt agagattatt tcactagctt tttggacatc
     2641 ttccttagtg gcatcaggaa catcttttag ttcataatcg atacctaaac tttcccattt
     2701 gtgtattccc atagaatggt atggtagcaa ttcaaatctt tctacatttt tcaatgtagc
     2761 tacaaattga cctagttttt ctaaattatc tatatcatca gtaatgccag gaactaaaac
     2821 atgtcttatc catacaggaa tatttcttga ttctaagtgt ctagcaagtt ttaatgcctt
     2881 atccatacca acaccagtca aatcaataga tttttcctca accatatgtt ttaaatcaag
     2941 taaaaccaaa tcagtattat ctaaaactgg gtctatagtt tctatatcta caaatccaga
     3001 tgtatccaag caagtgtgta tatcattttc ttttgctttt ttaaataatt cacttaaaaa
     3061 ttctggttga agagtggatt caccaccaga ggcagttata cctccaccag aaaacttcat
     3121 aaaagaagta tacttaagag catccgttat tatttcatca actgtgtatt cttttcctga
     3181 tttaatatcc catgtatccc tgttatgaca gtatttgcat ctaagaggac acccctgaaa
     3241 aaataatata tatcttattc ctggtccatc tacagtacca aacgtttcta tagaatgtat
     3301 ctttccctta atcatagagt tcacctcaca atatatttta ataaccttat caatagttat
     3361 aatatttctc taaaaaaggc catcttatac cagaaataat tcaaatataa gatagccttt
     3421 tatttttatt ttttacacag tagtaatctt agttaaagta taaaaattta aagactaagc
     3481 cattttaccg tggaatgttc tgtttataac atctaattgt tgctctttag ttaattttat
     3541 gaagttaact gcatatccag atactcttat agttaattgt ggatattctt ctggatgttc
     3601 catagcatct tctaaagtag ctctatcaaa tacatttaca tttaagtgat gagcattttg
     3661 agcgaagtat ccatccatca ttgcagaaag attgtttatt ctttcagtca tatcttttcc
     3721 taaagcagca ggtactatag agaaagtatt agatatacca tcttgagcat gttcatatgg
     3781 taatttagca actgaagata atgaagctaa agcaccatta gtatctctac catgcattgg
     3841 gttagcacca ggagcaaatg gagcaccagc tcttctacca tctggtgtat taccagtttt
     3901 cttaccataa acaacatttg aagttattgt aagtatagat tgagtaggat aagaatttct
     3961 ataagtttta ttctttctta ttttattcat aaagctttca actaactcta cagctatact
     4021 atcaactcta tcatcattat ttccgtattt tggataatct ccttctattt caaaatcaac
     4081 agctattcct tgttcatttc taatagtttt aaccttagca tgttttatag cagataatga
     4141 gtctgcacaa actgataatc cagccatacc acaagccata gttctaaata tatctctatc
     4201 atgtaatgcc atttctaatg cttcataaga atatttatca tgcatataat gtattacatt
     4261 taaagtattt acatataatg ttgctaacca atcagtgaat ggttcaaatt tactcataac
     4321 ttcatcataa tctaagtact cagaagttat aggttcaaat cttggtccaa cttgaacacc
     4381 agatttctca tctacaccac catttatagc gtataataaa gttttagcta agttaactct
     4441 agctccaaag aactgcattt gtttaccaat tctcattgca gatacacaac aagctatacc
     4501 atagtcatct ccccagtatg ctctcattaa atcatcattt tcatactgta ctgaacttgt
     4561 atctatagat actttagaac agaaatcttt gaatccttga ggtaatttag ttgaccatag
     4621 aactgttaag tttggttctg gagaaggccc taatgtatat agagtattta acattctaaa
     4681 tgagttttta gtaactaatg ttctaccatc tattcccata cctcctatac actcagttac
     4741 ccaagttggg tctccagaga ataagttatt gtaatcagga gttcttagga acttaaccat
     4801 tcttaatttc ataacaaaat ggtccatata ctcttgtaat tcttcttctg ttacgattcc
     4861 agcttttaaa tctctttcaa agtatatatc taagaaagta gatgttctac ctaaagacat
     4921 tgcagcacca ttttggtctt taacagcacc taaataacca aagtataacc attgtacagc
     4981 ttcttttgag ttagttgctg gttttgatat atcaaatcca taagattcag ccattttttt
     5041 aagttcattt aaagctgata tttgctcagt tatttcttct cttaatctga taacttcctc
     5101 atccatgcaa gaaacttcta atgatttctt ttgctcattt ttatcttcta ttaaagcatc
     5161 aacaccatat agagcaactc ttctgtagtc accaattatt ctacctctac cataagcatc
     5221 tggaagacct gttattatac cagattttct agcagctctc atttctggag tataaacatc
     5281 aaaaactcct tggttgtgtg tttttctgta tttagtaaat atatctttta tttctgggtc
     5341 taattcatat ccaaaagctt cgcatgaatt ttcaaccatc tttataccac catatggcat
     5401 gacagctctt tttaaagggg catccgtttg taacccaact attgtttcta attctttatc
     5461 taaatatcct ggagcatatg cagctattcc agaaacagtt tttgtatcaa catctaaagt
     5521 accaccattt tctctttctt ttttaaatag gaccattgct tcgtcccata attttttagt
     5581 attttcagta gcaccagcta agaaagagtc atttccctca tatggagaat agtttaattg
     5641 tataaactct ctaacattta tttctttagt ccatcttcct gttttaaatc cttgccatgc
     5701 attcattaag tatttcctcc ttagatttaa atgataaata atactattaa aattaatttt
     5761 tatatactta atttatttta tatatttttt taaaaatata ttttctaata taataatatc
     5821 atattattcg tatcctgtat acataaaaaa taaaaaattg tcgctttaaa gtataaaaca
     5881 gattatatgt taataatgtg gtgaaattat acccatatat aaataaaata aaacaaataa
     5941 atagctaatt aaggagaaaa tattttttat tttttgttta ggataaataa attttattat
     6001 ggaaaaaaga aaaagcatac atcttcacaa attgaatatt tatttaatag ggggaggtga
     6061 tagattgttt atcacagtgg tattattttt agtaggtttc ctattaatta ctaaaggcgc
     6121 agatatcttt ataaattgca ctgtagagat aggtaaaaaa acgaatatat cagaaattat
     6181 acttggagct actatagtta gttttgcaac aacattacct gaatttacag tgtctctact
     6241 tgcttctatt gatggtcata ctactatgag tttaggaaat gcagttggtt ctataatatg
     6301 taatacagga ttagcattag ggttggttgt ttttataagt ccatttaatg tagataaaaa
     6361 aatgtttttt tctaagtctt tactattaat agtatccgtt atagttttaa ttttattgag
     6421 tttagatgga gttataacaa gaggtgattc attattactt ataataatat taatctttta
     6481 tatggttaat aattatagaa gtgtcgttgg aaaatctgat actaaaaata gaaatattaa
     6541 aaataacact attaaagaca tatctactaa aaaagggaaa aattatagag gtttttccgt
     6601 attagaaatt gtaaaaatac tattactttt tgctacagga ttgataatga tgataatagg
     6661 ttcacaaatc cttatagaga gtggagtaat aatagctagt tttttaaata tacctcaagg
     6721 tatagtaagt ttaactataa tagcacttgg aacttctctg ccagagatag tttcgtcgat
     6781 tactgctata agaaaaaatc atcatgaaat atcagtagga aatattttgg gtgcaaacat
     6841 attgaatata gtatcagtaa tagctgtatc agcaatacca aataacatac caatactatc
     6901 tcaaaataga caattggata taccattcat gatattatta ttattaatag taataatacc
     6961 aacacttaaa tctaatagac ttagtagaat acaaggtata ttgatgttat tcacatattt
     7021 tctgtacata tcaattttgt atttcatgta tattatttaa ctatactgtt taaaatatta
     7081 attgcagaat atcctaaaaa agtatataat ggataggtta agattaattg ataacggaga
     7141 ggaataatat atgaatataa caaagtttga agatttacca ataagtgagg gaataaaaaa
     7201 agctatagct gaaatggggt ttgaagaacc atctcctata caagcacagt ctatacctgc
     7261 gattttgtca ggtaaagacg ttataggaca agctcaaact ggtacgggaa aaacagctgc
     7321 ttttagtatc cctatactag aaacaataga cccaaataat agaagtttac aagctgtagt
     7381 gctttgtcca acaagagaac tggcaataca agtatcaaca gaaataagaa agttagctaa
     7441 gtattcgcac ggaataaaga cattacctat atatggtggt cagccaatag atagacaaat
     7501 aaaatctcta aagagtggtg tgcaagttgt aataggtaca ccaggacgta caatagacca
     7561 tatcaatcgt aaaacattaa agatggataa tgtaaaaatg attatattag atgaagcaga
     7621 tgaaatgcta gatatgggat ttagagaaga tatagaaatg attttaagta agattccaga
     7681 agaaagacaa acaactttct tttcagctac tatgccaaga ggaattttgg aattaactaa
     7741 gagatatcaa aaagacccag agcatataaa agttgttaga aaagaactta cagtttcaaa
     7801 tacaaagcaa tactatatag aaactaggtc atcaaataaa ttagaagtat tatgtagatt
     7861 agtagatgta tatgacccta aactatctgt tgttttctgt aatactaaga gaaaagcaga
     7921 cgaattggta ggagatttac aagcaagagg atattttgca gatgcattac atggtgactt
     7981 aaaacagact caaagagata tagtaatgga taagtttaga aatggtacaa tagatatact
     8041 tgttgctact gatgttgcag ctagaggtat agatgttgat gatgttgaat gtgtattcaa
     8101 ctatgactta ccacaagatg aagagtatta cgttcataga attggtagaa ctggtcgtgc
     8161 tggtagagaa ggtatgtcat ttacttttgt atttggaaaa gaaatgagaa agatgaaaga
     8221 tatagaaaga tatactaaat ctaaactaat aaagcataat ataccaacaa taactgatgt
     8281 tgaagagaaa aaagtaggta ctttctttgc acaagttaaa caaactatag aagaaggtca
     8341 cttaactaaa caattacaat ggttagaagg tttctgtaat gatgaagatt atgcaatggt
     8401 tgatatagct gctgcacttg taaaactatc tctaggtgaa gaaatgaaag aagaaataat
     8461 agaagaaaaa cctagaagag agcgtggaga tagaaaaggt ggtactggag ctaaagatgg
     8521 tatgattaga ttatttatca atattggtag aaatcaaaga gtacaagcaa aagatatagt
     8581 tggtgccata gctggagaag ttggaatacc aggtaaagta gtaggtacta tagatatata
     8641 tgataagtat acttttgttg aaatacctaa aaaagatgca aaaacagtaa ttgaaaagat
     8701 gaaagatatt aagataaaag gtaataagat taatatagaa aaagcaaaca agaagaaaaa
     8761 ataacgtata gtatacaaaa ctataattag aaaatatata aaataatata ttggatattt
     8821 aaaaagcctg atgtgttttc aaaacagata gtagataatc tgtaagagtt aacagcataa
     8881 ggcttttctt gttttatatg atgttataat tttccagatt gttgtgaaaa atgtatatta
     8941 gttccttttt ttcaaatctg atttgaaaaa gagaatatta attatctata tagtttagat
     9001 tataataaaa aatgtaagat aacaaagggg agaaaaagat gtcaatgact tatatggacc
     9061 ctagggtttt tttgatttta gaaatggtgt tagaagaaga cacaaatact ctagaacaat
     9121 tttcaaatgt tttgggtgtt agtactagga ctatcagaaa ctatataaac cagattaatc
     9181 gagaattaaa aggtggtatt gctaaaatag ttaaaaatga aaaaggtaca tatagtataa
     9241 gaattgagga tgaaagtaaa ttaattgaga ttgtaaattt taatagagga aagctatcaa
     9301 attttataaa tttaaattca cctgatgaaa gaataaatta tgttcttgat attttaacta
     9361 tgaccaacaa tccaattacc atagatgatt tggctgaaga gatatgtata ggaagaacaa
     9421 cattaataaa ggatttaaaa aaagcagaca gaattttagc tgaatatgat ttagaattaa
     9481 aaaggaagcc aaatataggt atgattttag ttggtgaaga aatggatata agattattaa
     9541 tccttgatag attgtatgaa aattatattg atgtcttgga aaacgttaat cagataaatt
     9601 tagttaaaga ttatgatatt gaatgtctta gagatgaatt aaaaaagtta tttaaaaaag
     9661 aagaactcta cataacagaa caggtcttga gagatgttga aagatatata atcatgtctg
     9721 tcttaagaaa tctaaatggg tacaaatttg aaaagataga taagagattt gaagtgataa
     9781 gatgttcaga tgaatatact ttaggattaa agctcaaagc tttattggaa aagatattta
     9841 atatagtttt aaatgaaaaa gaaaccatat ttttgacaat gccattaata ggtagaaaag
     9901 ctcctagtac aaaattagca cttagcagta taaagataaa tgatagcgta aaggaagttg
     9961 tagatgaagt aactagtttt ttattagaga cctcagggat tgattttaaa gaagataaaa
    10021 agcttataga aaatttggaa tatcacttgt attttgcact taatagaatg agattcaaca
    10081 taagggtaaa aaatcctctt ttacaagaaa taaaggaaag atatacattt ccatatagtg
    10141 ttgcaaagat agctgcaatg ataattgagg aaaagtttga cttgaaaata tgtgaccacg
    10201 aaataggata tatagcatta cattttggaa gttattttga aaaaaattca agaaaagttc
    10261 taaatgtaaa tagggtagct gttgtttgtg gaacaggtct tgggactgca caacttatta
    10321 gaataaagat aggaaaaata cttggagaaa gtgtagaagt aaatactttt tctgatatag
    10381 aagctaaagc taatttagat ttattaaagt attatgattt agtatttaca acagtagatt
    10441 tcaaaacaga cttagatgtt ccaattttta gagtaaatgc gttatttaat gaagaaagta
    10501 taaagaaaga gatagaaaat gcaatacttt tgaaaaatag tataaatctt aagtctataa
    10561 aaaagattga aaaaaatatc ccttttatag gtaaattaat tgaagaggat aagttcttta
    10621 tattgaataa gagtacgttt atggaaaatt tagaagatat gttagaaaaa ttagttcata
    10681 aaggaattat tgatatagag ttcaaaaaaa aggtattaaa aagagaaaag aaatcttcaa
    10741 cagcattaga taattatata gcattgcctc actcagttaa tactaatggg gaaaatcttt
    10801 atctggcatt tggaatatta gaaaaaccag tgatttggga tacaaaggaa attagaataa
    10861 taatattgat gataattcca gataagaatg tagatagcac agacctgata ataaaaagtt
    10921 atgaagaagt acttgaagta gggagagata aaaagatggt agaagaactt gctaaggtaa
    10981 aatcatttga tgaattttat aaaataatta ccaagaggag gtaataatat ggacccagta
    11041 atttttatta tagcatcact tattacagtg tatttattaa atatttttct tggatactta
    11101 cagcttaaag attttaataa aaattatatt gaactaaaaa gaaaaggaag agtggcaatt
    11161 ggcagaaaaa aaggaagaat ttcatcaggt acaattgtgt tgatacttat aaatgatgat
    11221 ggtgtgattg tcgagactag aaaaatgcaa ggtgttacag tattggcaag agtaaagtca
    11281 tttgaaggtt tggtaggaaa gagcttagag tatatagaaa aatcagattt aactgtttac
    11341 aataaactac ttaaaaaagc aatactagat gcagtcaaac agtacataac atttgagaaa
    11401 aataaatgtg aagatacaaa taatctgagt actgatgaag agtgtaggat ggaagtagtt
    11461 taaaaaaata tatttataag gaggagttaa tatgacgcaa ttttttcaag tattagcaag
    11521 tggtgcagat agttttatga atttatttag ggcaggtggg caacaatttg taggttttgt
    11581 aacagatata gtaccacttt tagtagcatt attagttaca atgaatgcaa ttatcaactt
    11641 tattggtaca gatagagtcg agagacttgc aaaaaaatgt tctgcaaata tatttactag
    11701 atatttgata ttaccagtcc ttggtacatt tgtatttgca aacccaatga cactatcatt
    11761 aggaagattc ttgccagaaa agtttaaacc tagttacttt gcagcagcaa gcttttcatg
    11821 ccatacaatg aatggattat tcccacatat caatccaggt gaactattta tatatctagg
    11881 aattgcaaat ggtataacaa ctttgggatt ctcaacagca gatttagcag ttagatattt
    11941 gttagtgggt atagttgcaa actttattaa aggtgtagtt actgactata caacaaaatt
    12001 tgtagagaaa caacaaggaa tagcattaaa ttcagatata aagattggat aattacttta
    12061 aggtataaat aaatttttca aataaaaaga tagtgtatag aggaggataa attatatggc
    12121 atttaacaaa gttaaaatag taaaaggttc tggaggatgg ggtggaccat tgataataga
    12181 accaacagaa aagaagaata aggtagtata tgtgactggg ggagcaaagc cagaaactgc
    12241 agtaaaaata gctgaactta caggatgtga gttaatagat ggattttcac agggtgttaa
    12301 agatgatgaa atcgcatgtg taataataaa ttgtggtgga acattgagat gtggtatata
    12361 tcctcaaaag aaaataccga ctataaatat aatgaaaaca ggaaaaagtg gaccattagc
    12421 aatgtttata aaagaagata tatatgtttc aggggtaaaa ccaacaaatg tagagcagtt
    12481 ataattgagg tagtaggagg gaagaaatat ggcttatgat actaaaaaga agataagtga
    12541 gcagagtcaa ggaattgttg caaaaatagg tatagcacta ggaaatgtga caagtactct
    12601 ttttcaagct ggtagggata ctatagatac attaatacat actatactgc cttttatggc
    12661 tttcgttgca gtcctaatag gtataataaa tcaatcgggt ataggaaatt gggtagccaa
    12721 tttacttact ccattaatcg gaaatgttgg aggtttagtt gtcttatcaa taatatgttc
    12781 atttccgttg ttgtctccgt ttcttggacc aggagctgtt atagctcaga tagtgggggt
    12841 attggttggt acagaaatag gaaaaggaaa tattccacca agtttagcat taccagcatt
    12901 gtttgcaata aactcacaag cagcttgtga ctttatacca gtaggtcttg gtcttgcaga
    12961 agctgaacct gatacagttc aagtaggagt tccgtcagtt ttatattcta gatttttaac
    13021 aggtgcacca actgttctat tagcatggtt agcaagcttt gggttatata aatagaagaa
    13081 tactttataa ataattagag agaggttcag ttgatatgga attaatatac agtactcaaa
    13141 taaataaaat aggagataat gcaaatgaat ttttacaaca taatatgttt gtaactttca
    13201 aagataatgc tcctcaagag ttagaaaatt actgttatat acattctgaa aataatttag
    13261 taaaagaaat acttacggat gatgtgctat caataaataa tgttgattac aaaattactt
    13321 ctgttggtga gttagtaaac caaaatcttt cagaattagg tcatataaca tttaagttta
    13381 caggtgaaaa agatggagca attggaggaa ccttatatct tgaagaaaaa gaaatagcac
    13441 ctattggcgt aggtactata ctaaaaataa ttagaaaata aaatttaaaa cgaatctaat
    13501 catattataa gtaaattata gataaaagtc aatttaacta atcaagttaa cttttaatgt
    13561 gataaaaaag gggaaatgaa aatgagaaaa gtggctttag tcgttggagg aggaagaagt
    13621 ttaggtgaat atttatctaa acatttagct aaagtaggtt atgatgttgc tattgcagat
    13681 ttaaatgaag acaacgcaaa aaaagtagct gaagaaataa aaactgaatt tggttgtaaa
    13741 tctgtaggat atagttgtaa tgccatagat gaaagacagg ttataagtac tgtcaaagct
    13801 attgaaaatg attttgatag aatagattta ttagtgtaca atgcaggagt ggctgtaagt
    13861 aaaaaaataa ctgattttgg atacaaagat tttaaattta ttgtagacgt aaatttaaat
    13921 gggtactttc tatttgcaag agaagtatct aaagtcatga tagcaaaagg tattgagggt
    13981 agaataatac agataaattc aaaatcaggt agagtgggaa gcaagcataa ttcaggatat
    14041 tcagcaggta aatttgctgg agtaggactt actcaaagtt tagcgctaga tttggcagaa
    14101 cataagataa ctgtaaatgc actgatgctc ggtaatttac ttgattctga tatgtttgaa
    14161 agtttaattc ctcaatatgc agtaaaattg ggaatacctg aatcagaagt caagcaagtt
    14221 tatatagata aagtaccttt aaaaagaggg tgtagattta tagatgttgc aaatatgctt
    14281 acattttatg cttcaaagga agctgaatac tgcactggtc aatctataaa tattactggt
    14341 ggacaagtta tgtaaggttt ttatggagtg tctcaagatg aactctttag ttcatgaggc
    14401 acttttttgt ataaaattaa atatattttc agcatttaaa caatgaaaaa gaggtttatc
    14461 tttattttga gatagcctca tttttgtaat taaaaaacat agtgtttaag taaacatata
    14521 agattgttag ttttttaaaa gagaattttt taataatatt atatttatga agtattttta
    14581 aacttaattt gataaaatat agctatgttt tatatactgt agttttgaac atagaaagtt
    14641 taataaatta tagtatatat gttaattttt aataaggtaa gaaagtagaa agaaggatat
    14701 tttatggata taataaaaaa attaagtcat gggtgtacat tagattgtca tgactgttgt
    14761 aaattcaatg tttatactaa aggaaataat gtagtaaaaa ttgaaggaga taaaaatcat
    14821 ccttatacaa aaggttttat atgcaaaaaa gggatggccc atttagatag attaaatcat
    14881 aaagatagaa taaagactcc tatgcttaaa gttgatggag tatggaaaga gatttctttt
    14941 gataaagcta tagaaataat ggcagaaaag cttacatact ataaagaaaa gtatacatca
    15001 aagtctgtca tgcattatga ccaatatgga agtgggtctg tgctaaaata cataggagat
    15061 atatttttta atttttatgg aggtgttagc agacataagg gtggtccctg ttggagtgca
    15121 gggatgcatg ctcaaaaata tgattttgga gtagctaaga gtcatgctat agaagatatg
    15181 ttaaatagta aaagtatatt tgtttgggga aaaaatccag catatactac aattcatact
    15241 atgcaaataa ttaaaaaagc taaagaaaaa gggataaaga tagtagtgat tgaccccata
    15301 tatacaaaaa cagctcaaat agcagataag tatgtacaag taaatcctgg aacagatgga
    15361 gctttagcta tagctatggc aaaaattata gtggaagata aattatatga tgaagaatat
    15421 ataaactcgt atgttattgg ttttgaagaa tataaaaaat atttatcttc attggaatta
    15481 agttttttaa ttgatgaatg tggtgttaaa gagaatgata taagagagtt agtggattta
    15541 tatactaaca aatattcaag tataaatgtt ggatatgggc ttcaaaaata taaaaatggt
    15601 ggaaacacaa taagagcaat agatgcttta ggtgctataa ctggtcaaat aggatttagt
    15661 ggtggagggg taaactatgc taataaagtt tatccgagtg ttataaattc tgacccttac
    15721 aatagccaat catatggtga agatagggag ttttatgtaa gtaatatcag taaatttata
    15781 gaagaatctt taaaaaatac atcaaataaa gtaaattatg catcagatga acttgatatg
    15841 gcattacata aagtaaatta catatcagat gaacttgata caacatcaaa taaagtaaat
    15901 tatgtatcag atgaacttga tatggcatta cataaagtaa attatgtgtc agatgaactt
    15961 gatacaacat caaataaagt aaattatgta tcagatgaac ttgatatggc attacataaa
    16021 gtaaatggtg tatcagatga acttgatatg gcattacata aagtaaattg tgtatcagat
    16081 gaacttgata tgacatcaaa taaagtaaat tatgtatcag atgaacttga tatgacatca
    16141 aataaagtaa attatgtatt agatgaactt gatataacat caaataaaac agattatata
    16201 tcaaatgaat tgtataattt atctaataag tcaatcaaag ataatattcc tataaagatg
    16261 gctgtgataa ctaaaagtaa tatgctgaat caactgccag atttagtaga attggagagg
    16321 gtgttttcaa aaattgaatt taaagtatgt tttgatatgt ttataactga tacagttact
    16381 ttatgtgaca tgtttatacc gtgtacaaat acactggaga gtgaagatat aatttatagc
    16441 tcaatgacca atccatatat aacctataat gaaagagctg taaagccagc acataaattg
    16501 atggatgaat attacttctt tatggagcta gctaaaaaga tgggattaaa tgattatcct
    16561 tttgtagaaa agagaactta tttagaaaaa gtgattgaac cacttaagcg ttttgacaaa
    16621 aatctggata tagaaaaact taaaaacaat tactttacta tacataaccc tgttgcttgg
    16681 gaagataaga agtttgagac tccatctgga aaatacgagc tttattcaga aagtataaaa
    16741 aacttgggaa taagtccaac acctgtatat ataagcaata aatataaaga aatagaagat
    16801 aaaaatattt cttttagatt attaactaat catcatgcag atactttatt tagtcagcat
    16861 tttatggaca aaaaatctat agctcaagca tatataaatc agagaatggc taaaaaagta
    16921 ggtattgagg agaaagatat tgtgatattg aggtcaaaaa aagcaaaaat aaatgtacaa
    16981 ataaatatag atgatggagt aggtaattat atagtaaaaa tgtatgttgg ttggtggaag
    17041 aaacatggaa atcctaatag tttaacagac actggtatat cagattttgg gggtcaggta
    17101 acatataatg aaagtatggt agaaataata cgacaaaact agttaaattt cattgaagga
    17161 ataaaaatat aattatagaa ttgattaaaa gttactattt ttacaaaact attgaaggag
    17221 ggatttttga tggtaaatta ttccttagaa cataagaatc tatacataga atttatgtgc
    17281 tcagaactag accaccatgt agccaatgaa ataagggaag aaatagataa cttattaagt
    17341 gtaaatcaag taaaaaatgt tgtatttaat tttgaaaata ttaattttat ggattcttca
    17401 ggtataggcg tcatcatagg tagatataaa aagatttcta atgaaggggg acgagtttca
    17461 gtaattaata taagttcacg agttaagaag atttttgatt tatctgggtt aaataaaatt
    17521 attggcattt atgatacgta tgaagaagct ctaagttctt tgtagggagg agtatgtgat
    17581 gaataatatt atggaagtta aattttcggc aatatctgaa aatgaaagtt tagcaagagt
    17641 tatagtagca tcctttgcag ctaagttaga cccaacagta gatgagttag tagatataaa
    17701 aactgctgtc tctgaggctg ttacgaatgc tataatacat ggatatgaag aagacagttc
    17761 taagtttgtt ttcttaagat gtgagataga aggaaatact ataaagatta tagttgaaga
    17821 tgaagggtat ggaatagaaa atgtggagaa ggcaatggaa ccactttata catcaaaacc
    17881 agaacttgat agatcaggaa tgggattcac agtaatgaaa agctttatgg acgatgtaga
    17941 ggtaagttct gtaaaagata atgggactag aattgaaatg actaaaaaaa taaatgtgcc
    18001 taaataaagt ccaaaagtga ggttttatta tggaagtaac tgttgccaga gaagaaaaaa
    18061 aacctcttct tagtcatgaa gagactctag aacttattga aaaagttcaa aatggagatg
    18121 aagaggcaaa agaaatatta atatcaagca atttaggtct agttaggagc gttgtatcta
    18181 gatttttaaa tataggatat gatagagaag atttatttca gttaggttct ataggcttaa
    18241 taaaatcaat atataaattt gacccaaaat ttaatgtaaa attctctact tatgcagtac
    18301 caatgatttt aggtgaaata aagagatact taagagatga tggtatgata aaagtatcaa
    18361 ggtcgttaaa gcaaatagct gtaaaggcaa agatggaaag tgaagcttta actaaaaaat
    18421 taggtagaga gccgagtata gaagaattag ctaaagcaat agatgtagaa aaagaagatt
    18481 tagtaatggc tatggaagct aatttcaatg ttgagtattt gcaaggagtt attcatgaag
    18541 aggaaggttc tccaatatgc cttattgata aaataagcat gaaaggagaa agtgaagaag
    18601 aaaaagtagt ggataacata ttgttaaaag acatacttgg aagactggat aagagagagc
    18661 gtcaaataat agttctcaga tactttgaag acatgactca aagtgaaata ggagagatgc
    18721 tcaatatatc acaagttcaa gtatctagga tagagaagaa agtattgtct aagttaaaag
    18781 aatatatatc gtgataatac ttatgatatg tagaataaca aaatataata aatatattac
    18841 taaaataaaa tgtaataact ttttatgtta agtttttaaa ttattaaaag atattaaaat
    18901 aataaaaagc attaaaaaag taaaaatata aaaacaaggt gtctttatgg aaaaatagat
    18961 tttttcttta ttaggcatct tttttatttt ttgaccaaac tttagatagt caaaataaat
    19021 aataggttta tttttttaaa attaacaaat aatactttag agaggtgttt agtatggata
    19081 aaaattataa aaaatatgta gaccaaataa gcccaaaacc tacatattta aaaaactata
    19141 cacttgcatt tattgtaggt ggaattattt gtatgatagg gcaagcaata aatgatttgt
    19201 atatgaaagt tggaggtctt gataaactag gtgctagttc tgcaacatca ataacattaa
    19261 tatttatagg ggcattttta actggattag gagtttacga cctaatagga aaaagagctg
    19321 gagctggttc tataattcca ataacaggtt ttgctaactc aatagtatct ccagcaatgg
    19381 agtataaaag agaaggctat gtattaggtg taggtgcaaa cttatttaaa attgcaggac
    19441 cagttttagt ttatggtata ggttcttcta ttttatgtgg aattatttat tatattttta
    19501 agatgtttta gatgattttt atttaattta aattttatta attatgaaga aggtataaaa
    19561 tctgaaaata gctactaaag taactaattg aaaaataatt gtgcagattt aattaaaatt
    19621 tttaattaat attaaaaatt tgaatagtaa aatgcaaaat taaaaaggtg tgaaaatatg
    19681 aaaaataaaa gaattggaaa aagaacagtc aagttagaaa ataaaccaac cataatatca
    19741 actggaacta tagtaggtcc taaggaaggt gaaggaccac taaaggacta ttttgatatg
    19801 attatgactg acgatttata tggtgaaaag acttgggaat tagctgaaag taaaatggta
    19861 gagactgcat ctcaacaagc tatccaaaag gcaggtaaaa aattgtcaga tgtaaattat
    19921 atgctaggtg gcgatttaat aaatcaaata gttccagcat catttgcagc aagagaactg
    19981 gctattccat ttttaggtat atatggagct tgttctacaa tggcagaagg tctttgtata
    20041 ggttctatgc ttatagatgg tggttttgct gatttagtac tgtcaggtac atcaagtcac
    20101 tattgtacag cagaaagaca atttagattc ccacttgaac ttggaaatca aaaaccaatg
    20161 acagcccagt ggacagttac aggagcagga agtgtattat tagctcctaa tggagacggt
    20221 ccaaaagtaa agtatgttac tgttggtaaa gtaatagatg agggtattga tgatggtaat
    20281 aatatgggac cagctatggc tccagcagca atagatacta tctactctta ctttgaagat
    20341 actaatgatg acccaaatag ctttgatata attgctactg gagatttagg tactcttgga
    20401 aaacagatag cagaagactt tctaaaagaa aaaggtgtag atatatcaaa agtttatact
    20461 gactgtggta tagagatgtt taaccttaaa gaacaagatg tccactgtgg tggaagtgga
    20521 tgtgggtgtt cagccacagt gtttgcagga tatatatatg ataagttgag aaaaaaagaa
    20581 tttaacaaag taatgcttgt atcaacagga gcactacttt caccgacaag tacactacaa
    20641 aagcagacaa taccatgtgt tgcacatgca gtagtaatag taaatgagta ggaagtgata
    20701 taatatgtta atggactatg taagagtatt tattgtaggt ggaattatat gtttaatagc
    20761 ccaagtaatg atggattatt tcaaaatgca aactccatat gttatggtaa cgtatgtaac
    20821 aactggtgta gttttaactt ttctaggact ttatgagcca ttagttaagt ttgcagcagc
    20881 aggtgcaaca gttcctataa taggatttgg atattcactt gctacaggag taataaaatc
    20941 aattcaaagt gatggttttt taggtatttt tttaggaggt acaactgcta cagctggtgg
    21001 aatagcagca gctatattct ttggatatat tatgtcagta gtatttactc caaaagcaaa
    21061 accataaatc tttattaaaa gaataataag tttttgaaat aataaatttt tataaagcag
    21121 gtggatatct tttagattcc acctgttttt tatataaaga agcgtttaca taattgaagt
    21181 tgacaattat ttacaacata gtgtagtatt atattatcaa aataaaatga agtgaagtag
    21241 gggataaaga tggtaactat taaagatatt gcaaggaatc taggtatcag ttattcaact
    21301 gtttctaggt gcttaaacaa taatcctaat gtatctgaaa aaacaaaaaa gaaagtggta
    21361 gaagaggcaa atagactagg gttccatttt aatgtaaatg caagaaattt agcaaaaaaa
    21421 gaaactaaca ggataggggt aatcttttca aataatttta atcatcaaga tactagaaag
    21481 ttttttagtg atataatgga tagttctata aattctattg agactaataa atacgatttt
    21541 ataatacagc ctaataataa cattagtggt gatagtaatg tatataaaat ggtaaatgga
    21601 caaatggttg acggattagt aattgtgtct caatcaataa aaaaagaaga gtatgatttt
    21661 ttaaaagaca ataattttcc acatgtattt atatatttta aaccatcttt tataggagaa
    21721 gtagataatt tcttttggga cgataacgtg tatggaggtt atatagctac caagcattta
    21781 attgaccatg gtcataaaga tataataaca gtaacttcag atgataagag ttcaaaaatg
    21841 catgaagata gaaccaaggg atatttgaat gctatggaag aagcaaattt aaagacagaa
    21901 gtcattaaat ctaaaatgga ttttgaatct caagttgaat ttttagaaaa atacattgat
    21961 aaaataaaaa aagcttcagc tatatttgta caacaagatg taccagcgat ttctataata
    22021 caagagttaa aaactactta tggtataaat gtaccagaag atatttccat aatagggtat
    22081 aataatattg aattaatatc atattttagg tcgcatttat caacaataga tgacccaaga
    22141 gagcaagtta taaaaaatgg agtagatagt ttagtaaata taataaataa aaagagtaca
    22201 gaacacattc cgagaaaatt atatccaagg ttaataatac gaaatagcgt taaaagaata
    22261 aagtagtaag cttttaagta tcaaacgttt attaaaaata ttatagatta tgtgaagttt
    22321 tttaacaaat aatataaata aaaattaaaa ggagtaggtg tatataagta cattgtactc
    22381 ctttatttat tgagatagtt tttataatat gaatgaataa ttttattcaa ttatttgaaa
    22441 atccgttatt ttaacaatat aaattaataa attatttgta ttttatatca tgaatctata
    22501 atattttttt gattcataac taaaagtata taaaaatgat gaataaatat atattataca
    22561 ttatcaatga taggttatat aataaaatca ctaacaatta tattataaga aaattataaa
    22621 aattacaagg aggtatagaa atgaaagaaa aagcagggat tagaaatgcc ataatagcag
    22681 cctttataat cacaatattg gcagaattaa ttggacctat ttcatttaaa gttatgggaa
    22741 ttaatgtaac tttacttagt atactatggg ctatattcat aggaatggct gtttctcctc
    22801 atttattagg gagagttata ccagccttaa aaaagtttat aggggataat gaaataaatg
    22861 tatctccata tttattatct ttgacactat atccattagg gataatgttt ggaattaatg
    22921 cagggcctaa agttggtata ttacttcaag caggaccagc acttttatta caagaatttg
    22981 gtcatatggg tacaatgttg atagcattac ctgttggagt agcattaggt cttggaagaa
    23041 gtgcattagg tggtactttt tcattatgtc gtgacacagc actaggaatt attggagata
    23101 agtatggatt aaactcacca gaaggtatag gaacactagg aacttacata agtggtagta
    23161 tatttggaac attattattt tcatttttag caccagtggg tgtactttta ggatttcatc
    23221 catatgcttt agcaatggca tcaggaatgg gtagtggttc aatgatggga gctgcaacag
    23281 catcattaat gaatacggtt ccacaaatgt cagagcagat tttagcatat tcagctacta
    23341 gtggattgat tacagctgtt actggaatat atatagaatt attcttatct ttaccagtag
    23401 ccaactacta ttatagcaaa gtagctccag ttattgaaag atttagagga agaaaaacaa
    23461 gaagaacaag taataaattg taaaggagga tatatatatg gaaaaaactt ttaagactat
    23521 tatggagcag ataaaaatag tattgtttat atatattatt atttgctttg gacaaatgat
    23581 agcacttaag actcaggctc aagatttgtt gattggaagt gtatctggat ttttgttggt
    23641 gataattgct attataataa agaattttgt taaaaaacct aatttaccag gttttgcatg
    23701 ggctacatta gttgcatttg ttttatcgct tccaatatct ccagtggggg atatcatagc
    23761 aacaaatgta gggaaaatca attttatgtc tacagtaaca ccattattag cttttgcagg
    23821 aatctctgta ggtaaccaat tagatatatt aaagactttg agttggaagt tagtagttat
    23881 atcatttata gttatgacat ctacttactt tggttctgct tttatatctc aaacagtttt
    23941 aagattaaat ggaatgcttt aaggaggtag taaatatgaa tgaattaaaa gcaaaaatta
    24001 aagaggaaat aaagttatta tctgaagaga aaaagacatc atttgaaaaa gtttctgact
    24061 atttattttc aaatccagaa ttagcatttg aggagtataa gtcacaaaaa gcattatgtg
    24121 atttgttaga agaaaatgaa tttaatgtaa ctaaaggtgt aggaggatta gaaacatctt
    24181 ttgaagctgt gtattcaaat ggaactaatg ggaaaacagt tgctttttta gctgaatatg
    24241 atgcacttcc aggaatggga catgcatgtg ggcacaatat catagggaca agttctgttg
    24301 gtgcaggtat catattaaaa gaaatcatga aaaaacataa tatagaaggg actgtaaagg
    24361 tttttggaac acctgcagaa gaaagagtag gtggaaaaat aactatgatt aaagaaggtg
    24421 tatttaataa tgtagatgct gcattaattt tacatccaag tgatgcttca atgcctgatg
    24481 atatatcatt tgctcaagtt aatttaaaat ttgattttac tggtaaggca tcacatgctg
    24541 ctgcttttcc ttgggaaggg aaaagtgcct taagtggagt aatagcacta tttaacagtg
    24601 taaatagcat gagattacat cttaaagatt atgcaagagt acatggaatt ataactgatg
    24661 gaggtagcat acataatata attcctgaaa aatctacagc tatatttaat gtaagagctt
    24721 taagcataga atatttaaat gagatttgtg aaatgcttaa aaattgtgct aaaggagctg
    24781 caatttctac tggaactaat gtagaaatag ttcaattaga tgaaatatac aaagagatta
    24841 agaatgattc tgaattagtg aatatagtta gacaaaattt tgaagttttg ggtgaagact
    24901 atgttgaaag agatttaagt caaggtatag gttcaactga tacaggaaat ttaactcatg
    24961 agatacctgc aatacaagca tatataaaat taaaagaaaa tactgcaact catacagatg
    25021 aatttgctgt tgcagctggt ggtgaagaag gcagagtagc acttataaaa gcaattaagg
    25081 tgttagcaat gtgtggagta gatattttgt atagtaaata atatataatt ttaaaatata
    25141 ttaagaaagg aaatcggatt agacatgata ttaaaacaag taatagaggt ttatgacatt
    25201 ttagataaag caaatgctaa tggggaagaa gtaaaaactt acttacaagg ttatggtgaa
    25261 gtagatgtaa ctgtaaaaga attgtcttca agtaaaggct caacagattt agtaaagctt
    25321 acaattcctg ggaaaaatgg aaaattaaaa ggtggagatg ctcctactct tggcatttta
    25381 ggtagactag gagggattgg agcaagacca gaagttatag gttttgtttc agatggtgat
    25441 ggagcattgg tagcaattgc agtggctgct aaacttttgg atatgcaacg aaaaggggat
    25501 atattagatg gtgatgttgt aatttcaacc catatttgcc cagatgctcc aactaaagaa
    25561 cactatccta ctccttttat ggattctcca attgatatga tgactatgaa tgaaaatgaa
    25621 gttgatagta gttgtgatgc aatactttct atagatacta ctaaaggaaa tagagtaatc
    25681 aatactagag gatttgcaat atcgccaact gttaaagaag gctacatatt aaaaactagt
    25741 gataaccttt tagatattat gcagactgta acagggaaat ctccatttgt attccctttg
    25801 tctattcaag atataacacc atatggaaat aatctgtatc acttaaatag tattttacaa
    25861 ccagcagtag ctacagatgc accaatagta ggagtagcaa ttacaacaga gatgcctgtt
    25921 gctggatgtg ctacaggtgc aactaatttt agtgatttag attcagctgg gagatttgca
    25981 attgaagttg ccaagctatt tggtagaaat aagtgtgatt tctatgataa agaagagtgg
    26041 gaaatgctta ttaagagata tggcaaatta aatagatttc aaacatttgg tatacagtaa
    26101 ttagaaaaat attaagttta tataatttgt gatataacta tttaagaata tataaacaat
    26161 taggaataaa agtaagaata aagcacttag ttaaaaactt taaaaataaa atatttattt
    26221 gtttatataa aaaggaggat tctggttatt gggattctct tttttatgaa tacaaataat
    26281 atattgtaat tataaaaaaa ggagcgtttc aaagtgattt ttttaatcac cttgagacaa
    26341 ctccttatat ttttatttaa attctatttt ttattaagtt gctggagttc caccaccatc
    26401 aggaggagta gtagagccac caccattatt tccaccatta tcaggaggag tagtagagcc
    26461 accaccattg tttccgccat tatcaggagg agtagtagag ccaccaccat tatttccgcc
    26521 attatcagga ggagtagtag agccaccacc attgtttccg ccattatcag gaggtgtaga
    26581 accgccacca ttatcacctg tgttattatt gttattatta ttttcttctg gttggttatt
    26641 gtctggtttt tttgtatctt cagatgatgg ttgagaactt actttagtag gagcagtacc
    26701 ttcaataaat gctgctttag agccagaaga ttgctttcct ccatcctcta aattaatttt
    26761 tgtaaaatat acatttttag gaacttcaaa ttcagtaacc gttaaattag catgtatttt
    26821 ttccataatt ttttcccata gttttgcagt actagtactt ccatgtggga cttctctacg
    26881 ttttatagtg ttaccagaat catcttttct accagcatca tctccaatat aagttgcacc
    26941 aacataataa ggagtgtaac cgacaaacca agcatcatat gcactatttg tagtacctgt
    27001 tttacctgct acaggcattc cctttggaag tgtagcaaca ccaccagtac cttcagtaat
    27061 tacgctttct aacatatctg taacaacata agctacttca gggtctacaa ctttatgttc
    27121 ttcaggagca tttttaacta ataattgacc atcaaaggta gttatagtag taaaaataga
    27181 aggttctaca taaataccgt gatttgccaa tgtaccataa gcagcagcca tttgaagtgg
    27241 agtaatacca ttcgccatac cacctagtgt caatgatggg aattttctat cacttgcttc
    27301 agatgaacct gagtgattat tctttaaagt tgttatacca aagttcttta agtaatccat
    27361 cattatatct acacattcat cataagagtc tccaagcatt tcagcagttt tgacagctat
    27421 cgtgtttgaa gatttagcaa gtgctttacg taaactcata gaaccatgac cagcagtagt
    27481 agttcttgga ttccattcat tattttcttt aaacttatat cctcctcgag aatcacttag
    27541 agcagttgct tgtgttattt ttagagtatc aatagctggc gtatatattg ctaaaggttt
    27601 tatagaagaa cctggctgat gagggttagt agcacgattt aaagtttttc taccacttat
    27661 atttcttcct ccagctagtg ctcttatttc accattttta taatctaaga ttaccattgc
    27721 agcttgcggt tggctaactc catcaggacc gaccatatgt cctgggaaat tgctatttct
    27781 atcaaattct tcttctaaag catcttgcat tttagggtct atagttgtgt ttacaattag
    27841 accaccattg tagaataagt tttgagcttc atcatttgta tgtccttgtt ccattaaagc
    27901 atttataact tcactttcga taaggtcttc tacagatgaa gaaactttat ctgatgattg
    27961 aggcaatttt atttctattt tagctttgat tgcttcatca tattcacttt gttttatata
    28021 tttcaattct aacattttct ttaaaacaat ttcttgtctt tttttagctt caggattact
    28081 tacagccttt ctaacaacta atgtacctgc ttttaattgt ctataagtat cattagatat
    28141 aagctcccaa gattttattt tttcaatcat atcaaagtcc acttgagtag ggtcatctaa
    28201 atcatcatca gttgtgttaa caaaaaataa taatttattc tctaaatcct ccttagtttc
    28261 atttccctct aacttagaag ttttataggc tgaaaatctt gatggatttt tagttgaacc
    28321 agcaagaagt gctgactctg ctagagtaag gtcagctgca gacttatcaa aataaccacg
    28381 tgcaccagcc tcagcacctg ctagacctct accaacgaag aagttattta aataagtttc
    28441 tagtatttta tctttactta ctgttttact catctcatgt gcatagtaaa tatcctttat
    28501 tttacgaggt atagtttgct gttcagttgt taacagcatt ttggatacct gcataggaat
    28561 agtactacca ccttgcttag tacctgtaag ggtctttaat actgacctaa ataaaccttt
    28621 aatatcgaca cccttatgct cataaaaacg ctcatcttct attgcaatta cagcatgttg
    28681 aagataaggg gaaatcttat ctaatggaac gggtgtcttt ttatttacac ttggagctgt
    28741 agataatgtt tttccattag cgtatttaat agtagttgtt tggttcgtct gtttatctaa
    28801 aacagcttct gtaactggag ttacatctct tagtgacgca aatactagac cagcagtacc
    28861 tgctgtacca acaactagta aaactaaaaa tactatccca aatactctta gttttttaaa
    28921 tttatcgctt tttttagatt ttttcttgtt ttttgttgaa cgagtcttgt tagctgaagt
    28981 tctattaatt ggtttatttg tattaccaga tgagctaact ttttttctcc tgattttatt
    29041 tccattttta tcattatcgt tattcataag attttaacct ccttacttaa tgcaatatta
    29101 taacacaaat aggagtagtt ttgtaattaa tagaaaaaat gaatttgcta taatattctg
    29161 tacattgtat atgtataaac gtaatatatt aaaaagtcta tatttttatt ttatttattt
    29221 ttgacaaata ttataaaaat tatgccaaag ctacatataa ataaatagga ggggataatt
    29281 ttgaatagaa tagtagatag aaaaacaaaa aatgaattta gaaaaatcat agctattttt
    29341 ttagtaattc ttttcttgtc tgtatttata ggcagtttta tatatataga taaaacttta
    29401 agaccgacaa taacagtttt agcagagact aaagctcttg aactagcaaa taggtcgata
    29461 aataaagctg ttgctgagat ggtagaagga aaaataaatt atgaagattt aatggatata
    29521 caacttgata ataatggtaa aataactatg atacaagcaa acactataat gatgaatgaa
    29581 attgcatctg caatagcttt agaaatacaa gatgaattaa aaaaggataa gacagcaagt
    29641 tcttacatac ctataggaac agcattagga agtcctatat tagctaaata cggtcctaaa
    29701 cttgaagtat ctattgaacc tataggaaca gtatcagtaa actttaaaac ggaatttgag
    29761 tcttcaggta taaatcaaac tagacataga atatatttag aagctcaaac acaagttaaa
    29821 gtagtaatac cacttataac ttcaacaaaa caaattaaag ctcaaatacc tatttgtgaa
    29881 actataatag taggtgatgt tccagaaagt tatgtaaata ttccagaaaa aaatctagga
    29941 aatgttttac ctaacactgg aaagaatacc aacaagtaat ataaagttgt atatttttaa
    30001 ataaattcaa aataatagtt tataaataaa atttttggaa ataatattaa tataatttta
    30061 ataaaaattt atattttata ctatgtaaaa tatctaatat atttggtctg taggttaaat
    30121 aaaagtacct ttagaccaaa tatattatta ataacttaat aacatcttaa aacttattta
    30181 tttatttata ttatttacaa catccattta ataaatacac cataacctac agctgggtcg
    30241 ttttcaattg catgtgacac tgctccgatt attttattat cttgaactat tggagtacca
    30301 ctcatacctt gtacaatacc accagtttga gctaaaagct ttttatctgt aattttaatt
    30361 ttaaaacttt tactttctgg atgtttttgg ttttctatat ctataatttc aatattatat
    30421 tttttacact tattaaagtt atcttgtaaa attatttgtg cttttcctaa tttaacttca
    30481 tctaaagatg ctactttata tttttttaac ttggaaatat caaagttatt aatttgacct
    30541 cttattccaa aatcagtatt ttctgtaaag ctaccaataa tattttctcg ttttgcattt
    30601 atacatccaa cactaccttt ataagatttt tctatattaa ggtgcttagt tgtagaaatt
    30661 gaacctgttt taataggtat ttttcttgca ttaccaacac ttattggatg acctacagct
    30721 ccaaactctg atgtatctgg attgatatac gttaaagtag caaatccaga aagtaaattg
    30781 ttaaaactaa ttttttctaa tatgtgtttg gtagttttca ttgttataag ttgtccacct
    30841 ctatttaata aaatagatac ttgtttttct tctggtaaag catttaaggt attggcaaag
    30901 tcactatatc catcaacttc agcattattt acctttacta tagcatcacc caatctaaat
    30961 ggagatgctt ctccaggatt tctaactatg atatttttta attctgcgtc aatttgaagt
    31021 acgtttccaa ttggtattaa atattcaggt tcattttttt gtaaattttg tgcataaatt
    31081 aaattatttg agaaaaaata taataaaaat aaaaataaaa atgttaaaac aattgtaaat
    31141 tttgacttag ctaagttggt ttttttactt aaagtattat ttttaaaatg aaaattttta
    31201 agttgcataa aacacctctt tcttattaaa ttattttaat aatagaattg aagttttctt
    31261 gctgcttcta tattatgttg tgcaagaatc atgttttgat gcttggaaaa ctagctcaat
    31321 aacaccttag ttttcataaa aataaataaa ataaatagtg tatatgataa aataaataca
    31381 tattcatata aataaaaata aacaataact gtctagcata agaaaggagc aaaaaatgaa
    31441 aaaatataga ttgttagtta cttgtataat tgccatggga atcataatta taggaacatc
    31501 aaatccagtt agcaattttt acaagactac aaatttgatt agttcaggaa atatcacaaa
    31561 tgaaaaagca aataaagatg aaaattcaaa caaaaataat aaagataaaa atgaagacaa
    31621 atctaattct aaaagtaata aagataagtc atcagaggac aaatcgtcag ataataaaaa
    31681 tgaaattcaa aaaaataaaa aacttttaat ttgtatagac ccagggcatc aaggaaaagg
    31741 agatagcaat ttagaaccag tagctccagg ttcttcctct aaaaaagcaa gagtatcttc
    31801 aggaacagag ggtattgcta caaaaaagcc agaatatgtt ttaaacttag aagcatctct
    31861 tgttttaaag agtatattag aatctaaagg ttataatgta ataatgacta gagagacaca
    31921 tgatgtaaat ataagtaatt cagaaagagc aatacttgct aatgacaaaa aagcagatat
    31981 ggttgtaaga atacatgcag atagtttaaa taattcttca aaaactggag catctatatt
    32041 gattcctgaa aaagatggaa aatatacagc tcctatatat gaagaaagta ataaatgtgc
    32101 tgaatttatc aaacagaata tggaacaatc aggtatacaa atcaatggaa tcgttcaaag
    32161 aggtgatttg actggattca actggtcaaa agtaccagct gtattagtag aaatgggatt
    32221 tatgagtaac tataatgaag accaaatgat gtcaaatcca gactaccaaa gaaaaatgat
    32281 gcagtgtata gctgatggat tggatgcgta ttttaaataa gatatgaata gagaaaaagt
    32341 taggttggta ttattttcct aatttttatc tctatttttt attgtacata acacaaagaa
    32401 gaagtttatt ttaggttaaa ttgtcgtaaa tataactaaa aatattggga atacttgatt
    32461 taagtttaaa aagaatatat tatagtatag aatgtgtaaa atttttttga gaggtgttga
    32521 aatgctagta gtagaaaatg tgagtcatgg atttggtgcg agaacaatat tagaaaatgt
    32581 atcctttaga cttagaaaag gtgaacatat tgcactagtt ggtgctaatg gagaaggaaa
    32641 atcgagtttt ttaaatataa taacaaagaa gcttatgcca gatgctggaa atataaaatg
    32701 gtcttcaaga gctacagtag gttacttaga ccaacatacg gtattaagca agggaaaaac
    32761 tataagagaa gtgcttagag aggcttttaa gcatatgttt gacttagagc aagaaatgat
    32821 tgctatgtat gacaaaatgg gagaagctag tgatgatgaa atgagtaagc ttcttgaaga
    32881 aacagcagag atacaaacta tacttgaaaa tagtggtttt tatatgatag atgcaaagat
    32941 acaagaagtt gcaaatggtc ttggtcttgg agaaattggt cttgataaag atgtaactga
    33001 cttaagtgga gggcaaagaa caaaagtact tcttactaaa cttctacttg aaaatccaac
    33061 tattcttata ttggatgagc ctacaaacta tttagacgaa gagcatataa catggcttac
    33121 taagtacctt caagagtatg agaatagttt tgtcttagtt tctcatgata ttgaatttat
    33181 aaataataca tgtaatgtaa tatatcatat ggaaaatgga gagttaaata gatataaagg
    33241 aaattatgat gaatttgtaa gacttaatga tataaagaaa cgtcaagaag aacaagctta
    33301 tgataaacaa gttgaagaaa gaaaaagact agaggatttt gtagctagaa ataaagctag
    33361 agtagcaact agaggaatgg ctaacagtag acaaaagcaa cttgataaga tggaaatatt
    33421 agaaagacca aaagaaaaaa taaaacctac ttttgctttt aaagatgcta gagcagctag
    33481 taaaataatt tttgaaacag aaaatttagt tttagggtat gatgaagcat tgacaaaacc
    33541 acttaatttc catttagaaa gaggaaaaaa gattgcctta aaaggaatga atggtattgg
    33601 aaaatctact ctattaaaaa ctttacttgg aataataaaa ccatttgaag gaaatgttaa
    33661 attaggtgac tatttggaag ttggatattt tgaacaagag agttcaagag aaaatagtaa
    33721 tacacctatg gatgaagttt ggagtgaatt tccagggctt acaaactttg aagttagaca
    33781 ggctcttgca aaatgtggac ttacaaatga acatataacg agtcaaatga gagtattaag
    33841 tggtggagaa gctgctaagg ttagactttg caaagtaatg ttaaaaaata taaatttctt
    33901 agtattagat gagcctacaa atcatttaga tgttgaggca aaagatgaat tgaagaaggc
    33961 tatcaaagag tttaagggaa cagttctttt agtatgtcat gagccagaat tttattcaga
    34021 aattgttgat gatgtatgga atatagaaga ttttacaact aaaatcgtgt agtgatttaa
    34081 cttattgctg gggggatttc atgaaaaagt ttctaaatgt acttttttca atttgtatta
    34141 gtattgttat aatagtaggt gttattaatt ttactgttgg atttaaacaa ctttattatt
    34201 ttgacattga ttacttaaat atatcagagt tatctggttt atctaaagat gatataaaat
    34261 tgaactacga ttatctaatt gattataact taaataagaa tgtgtctgag ttcaaattac
    34321 caacattaaa atcttctcca caagggaaga tacattttga agaagtcagg aatatatttc
    34381 aaaatataaa taaattagct aaattattat tggtagtttc tttagtagga attatattga
    34441 gtgtaaaaaa taaaaatata aaaatcttaa aaactacatc aataacactt ataattatgc
    34501 cattattatt aacagttcca attttattaa attttgaaaa gtcatttatt atttttcata
    34561 aattattatt tagaaatgat tattggatat ttaatcccga tttagaccca gttataaata
    34621 tattgccaga ggaatttttt tttcactctg gaatgatgat tttaatatta attttgttag
    34681 taagtatagt actgtttgta atgtataaat tatgtaaatc tttatcaata aaaatatttc
    34741 aatgttaaat agagggatat ttttatttcc aaaataagat aataaaaaag gattagatta
    34801 aaagttatac aataaaaata gctgtgcaat aatatttaga ctatgaaagt agttaaaaaa
    34861 tatttatgca cagctattta taatagaatt tactttttac tagtgatttt aactaaatct
    34921 tgaaatttac tcataatgta attgtaatta tcttttttat gaggtaatat acaactactg
    34981 caatctttga tacctttatc tgtatactta aagtttccac cacagttttc tcctaaagca
    35041 tacaaaggac aatagcaaaa tagacagtta aactcttctg gtttattagt tttatgacaa
    35101 gggaaaaatt cgcaatcttt gtgattaaaa aacttataat tttcgcccat attttcctcc
    35161 taattaaata aaatttaaat tttttatact atataagatt ttggtgataa tataattata
    35221 ctaaataatt ttatgaatag tcgataatat agtattatta atcatagtat taatggtata
    35281 attagtcaac agaaaaataa tgtaaaaata atgtaagtgt attttgtaat tagaatatgt
    35341 tttataacaa aaaatgaggt gattaagtga aagaaattca agaaaaagca cttcttgtag
    35401 gtctgaattt gactacaata gtgaaaaaaa atgatgatat agacactaat gaatctatgg
    35461 aagaattaaa agaactaaca aaagctgctg gggcagaagt tgtaggtagc ttaattcaaa
    35521 ataaacactc tgtagatgca gcttattata taggaaaagg taaagtagaa gaaataagag
    35581 cctatagtga ctctttagat gctactttag taatttttaa tgatgaatta tctggtgctc
    35641 aaatcagaaa tatagagaat gttgttggaa gaaaagtaat agatagaact actttaattt
    35701 tagatatatt tgctcaaagg gcacttagca aagaaggtaa attacaagtt gaattagcac
    35761 aattaaagta tagattgcca cgactttatg gaatgggtgg tgagatgagt agaacaggtg
    35821 cagggattgg tactagaggt cctggagaac aaaaactaga aatagataag agacatatac
    35881 ttaataaagc agcagatata agacgtgaac ttaaagaagt aaaaaagaat agagagacac
    35941 agagagttaa gcgtttaaag tcaaatatac cgattgtagc acttgttggg tatacaaatg
    36001 caggaaaatc taccctgttg aacgaattaa taaagacaca caaggattat gaacaagaaa
    36061 aagaagtttt tgtaaaagat atgctttttg caactttaga tgtaaccttg agaaaggctc
    36121 ttttgccgaa taaaaaagaa tttttagtag tagatactgt tggatttgta agtaaacttc
    36181 cccatgattt agtagaagcg tttaaggcaa cattggaaga agtacagtat gcagatttaa
    36241 tcttacatgt aattgatgca acaaatacta gttatgaatt gcaaaaaagt acgactgaag
    36301 gtgtattaaa agaacttggt gtaaatgata aaaaacatat acttgtatat aataaagtgg
    36361 acaagctaga attggatata tatcctaaaa gtcaagaaga tattgtgtat atttctgcaa
    36421 aacaaggcat aaatatggat aagttattaa atatgataga aattgcttta atggaaaata
    36481 cttattcagt aagtttgatg ttaccttatg agagaggtga tatatttagt agaataaaag
    36541 ataaatataa tgtggagaat tttgagtatg gggaaagtgg tataacgcta gatgtagatt
    36601 tagatgaaga agattttaat atctatagag agtacatact agaaaaatag gttgaatatg
    36661 gatataaact gggaaaatat agataatctt gaagattatt ttataaccta tcttctttat
    36721 aaagaatcta aaacagtatc tcaaattagt aaaattagaa atatttcaag tactgaagca
    36781 agtgagcaac ttatacaagc aaagttaaaa ataaaggaaa tgcaaaaaga tgattttgag
    36841 gcatctaagg atattttgga taaatttcta gaactagata aaattaagag acttgatttt
    36901 atggatagct tagatgacga aaaaatggta tactttaaga gaaaggtttt caaaagaatc
    36961 cttgtagaaa aaaatgctga agatttaatt gttttaattt gggcgacagg tgagctaaaa
    37021 gatgataggt ttttgaaatt gcttcatcag ctcacaaacc acagacactc agatattaga
    37081 agaataactt attcagcaat cagaaagatt gaatcaccaa gtagtagaga agtactgcaa
    37141 aaaggtctct acgacaaaaa tgcacagact aggcagtact gtgcaaaagc tctttcaaaa
    37201 ttgggagatg aaaacagttt aaagatttta cagcagttga aagacaagaa taagaatttt
    37261 gaaaaagaat atgtgcttag agcttatgac aaagccatta agtcattgga aaatgcaaaa
    37321 aatttgaaaa agtaattttt tattaaagca atttgtttac ttaatgatgt atttctaaag
    37381 tttaacaagg taataaataa attaatggga ggggtttcaa atgatagtaa gacgttgtgc
    37441 aggtggagta gtgttttatg ctaataaagt acttattgta aaaaatgata ggggtgaatg
    37501 gacacttcca aaaggaaaga tactaggagg gggacttcca tatgagagtg ctgttcaaag
    37561 agttaaagta gaaactggca tagatgctaa aatgatagat gtggctggcg atactatgta
    37621 tgaatttttc tcaagaagta gacaacaaga agtttgtaat gcaataatgt ggtatgttat
    37681 ggaagcttgt aacactgatt gtgtacttgc tcctgaattt caagaaggtg gattttataa
    37741 agtaaaggat gctttagaaa tgttatcaca tcataaagag caagctttag tagaagtatc
    37801 ttataaaaag tttaaggaat taaagaaatt agtttcagag ggcgatgatg ttccagcaaa
    37861 ttaattgtga atttagagga ttctgaggaa gggatagccc cttccttttt taattgaagc
    37921 agttttaagc gtaaattata tagtctcttt aaactatgat ttatcaattt agagatttag
    37981 tagtttatat ttaaaatcag taagatatat ttaaaataaa aattaaaaag tatacaattt
    38041 tattatataa ttgtcttatg atgttgatta aagaattatt aaaagtccat ttatttttaa
    38101 atagaataat tttataaata tatatttgat gtgtatatta gaaaatgatt aaaataggag
    38161 gaaatacaat gagcaattac aggacattac atgaatttgg aatggatgaa ataacgattg
    38221 aaaagtctgt ttttattgga tatgctaagc ctataaagag tgaagaagaa gcattagagt
    38281 ttataaatga aataaagaaa aagcataaag atgcaactca caatgtgtgg gcttatactg
    38341 taggtaaaaa tatgaatata caaagatata gtgatgatgg agaaccacaa ggaacagctg
    38401 ggatacctac attagaggtt ataaaaaaag aagatttgag agatgtagcg gttgttgtta
    38461 ctaggtattt tggaggaaca aaattaggtg caggtggtct agttagagct tatacaaaag
    38521 gtgctaaact gggacttgaa gcaggtaaga taatttataa ggtaatgtat caagaagtta
    38581 aggttaagat agattatact caacttggaa aagtgcaaaa tgagttaatg aatttggggt
    38641 attttataaa agatactgta tatgaggata atgttgaaat agtagtatat tcaagattag
    38701 aagatgtaga gaaattatca gaaaaaatga ttgatataac aagtggaaca ggaaaaatag
    38761 ttttaggaga ggaattttat ttgtctgaac aaaatggaga aattttaata taatctaaaa
    38821 ggttattaga atagttaata cctcaaatag ccaattagta tttttaagtg aaaagccatt
    38881 aaattgattt aatctaaatc aatttaatgg ctttttatcg gattttgcac agaatccttt
    38941 ttttacttca aataaaccga ctaaaagtaa aaatatccca aatatttttt ttaatatatc
    39001 tgaagacaga taattagcta atagtgaacc aaatacagct cctaaaaccc caaacacagc
    39061 tataggtata accaatttaa aaattacatt tttatttttt atatgtatag caagtgctat
    39121 aagtgtcata gggatagatg aaagtaaatt tacgctttgt gctattttag tgtctatgcc
    39181 agcaagtaaa acgagtgcag gtataaggat tgttcctcca cccattccca ttcctccaat
    39241 aattcctgca aaaaatccaa ttagagcaaa taacattaaa acaccatcct taatgctgca
    39301 attatcataa ttattccaaa agaaattcta agaaactttc cagtaacctt atttagtaat
    39361 ttagctccta taataccacc aagaatactt ccaactgcaa ctttaattgt taagttaaca
    39421 tcataagtac ctttagatac atataaaaca gaacttgctg tcgttaagaa tattattata
    39481 gaaagagcag ttgcatgaga tttatgttct tctaccttaa cgatgtcatt taatattgga
    39541 actaaaagag tacctcctcc agaaccaaaa actccattta taaatcctgt aaaaactcca
    39601 attatcgtat tttttaagtt aaaatcaaag tattttttct ttaaatcatc agtttttttc
    39661 ataaaaatca ccttctgaaa tagttttacc aaaataggca aattatattt atataaaata
    39721 agattaaata taatctttca tgtttgttta attttgcata aaaaaaaaca tattaaaaca
    39781 acatattaat atatggtata atatattaaa acaaagtagc aaatttgaga ggagataaca
    39841 atgtcaaata taaaaataca aatagacaaa actgtagaat ggcttataaa taaggttaat
    39901 gaggcaaatg caaaaggatt aattgttggt gtatctggag gaattgattc ggcagtagtt
    39961 gctaatttaa ttaaaaaggc atttccagaa aactctatgg gagttataat gagcataaaa
    40021 agtaatcctc aagatagaga agatgcactt aaggttatag agggatgtga tatagaatac
    40081 cttgatttag accttataga accacaaagt gctatattag acatggtagt aggaaattta
    40141 aaagataaac atttatatag agaagaatat ttaaagatga cagatgcaaa cttaagagca
    40201 agagtaagaa tgagtacaat ttatacaatt gctaacaatc taggatatct ggttgttggt
    40261 acagacaatg ctgcagaaat acacacagga tattttacta aatttggtga tggtggagtt
    40321 gatattttac ctattgcaaa tttaactaaa ggagaggtgt atgaatgggc aaaagagctt
    40381 ggtgttcatg aagatttaat aaacaaagct ccttcagcag gtctttggga aggtcaaaca
    40441 gatgaagatg aaatgggaac tacttacaat atgatagatg ctgtgttaga aggtagattg
    40501 gatgaagttc ctaaaagaga ccaagagata attgaaagac ttcatagatt aagtgaacac
    40561 aagagaaaaa cgcctgcaca acctcctaag ttctaattta ctatttataa gatttgtgat
    40621 aaaatttaca agtgatgaaa attagaataa ttaattgtac gtattaagga gtggaaatat
    40681 gggacgtata ggtaatataa ttaatagaaa aggtaaacaa gatgctcaaa gagcaaagat
    40741 atttactaaa catgctagag caatagcagt tgcagctaag gaaggtggag cagacccaga
    40801 gtacaatgca gcgttaaaaa cagctataga aaaagctaag gcagataata tgccaaatga
    40861 taatatagat agagctatcg ctaaaggtgc tggagcaggt gctggagaag actatgaaac
    40921 tatagtttat gaaggatatg gacctggtgg agttgctgtt atagtagaaa ctttaactga
    40981 caacaaaaac agaactgctg gtaacgtaag atactatttt gacaaaaacg gaggaaactt
    41041 aggaactagt ggatgtgtgt cttttatgtt tgataaaaaa ggacaaatat tagttggttt
    41101 aggagatgga gtttcagaag aagaattgat ggatgttgca ttagaagcag gagctgaaga
    41161 tttcataaca gaagaagatg gttatgaaat aataactaca ccagaagatt tttctagtgt
    41221 tcgtgatgag ttaaaagcta aaggatatga gtttatatct gctgatgtga agatgatacc
    41281 tcaaactaca actgtattga ctgaagaatc tcatttaaaa atgatgaata aattagttga
    41341 tatgcttgaa gaagatgatg atgttcaaga tatatatcat aactgggaag tagaataaat
    41401 agtataagca tattgaaatt tataagtatt gaatcaattt taacatgtga taaattttat
    41461 agaataagaa acactaaaat agacatacgc acctctaacc tatatttgca taataatatg
    41521 taagcatagg taaggggtgt ttttatattt ataggttaag aaatttaaag taaattttga
    41581 gctggactcg attgtctata aataattgat gggtatataa cttgttgctg aataccattt
    41641 atacgcccag ttactgtatc aataatcatc ctaagactac gttctgctgt ctcttccatt
    41701 tttaaatcta taactgtcaa aggaggaata cttcttgcca ttagtcgtga aggtccatta
    41761 cttgctgcaa atatttgaat atcctctcca attacaattc ccgattcatg aaatgcattc
    41821 atcataccaa gagctaccac atcatgatta caaaaaatta ctttttttag tgaatttttt
    41881 gcaagcattt tttgagcaat atcatatcca ctatctattt tattagaaca ataatatata
    41941 gaatctgata gatgtattcc atgttcttta catgttttta aaaaagattt accccttaaa
    42001 gtcatacatg taaaggaagt tgggttgaat acaacagcaa tatcattttt accattttga
    42061 atagcatgta aagctgctat acgaccagtt tcctcattat caatagatac acttgaaaaa
    42121 ccaggtattt cacgatttac aacaactaca ggaattttag gtttattttt tactagatat
    42181 tctatatcat cagcattgat acctgttaaa acaattccat cataataatt atttgcaaaa
    42241 aagtattcat catcacatag atgaccaact ttgtaaggtc gtatgatgat atttatgttt
    42301 aaatgttcta aagaaattat gtggtttatt gcttgaagta tattggttga tgccaactca
    42361 aatccctcat cctgccaata aaaagctatt ttaatattat tatgtttaat aggagtagag
    42421 cgtttatatc caagcttatc agctgaattt aatataagct cttgtgtatg tagcggaatc
    42481 ctgcgttcat ttcctttacc acttaagacg tacgagacag ttgctgaaga aagaccacag
    42541 tcttttgcaa tatctttgat tgttgccatg ataaaatcct ccttaaatag ttgcttaaat
    42601 ttgcttatac caataatctt aacatatatt caactaaaga acaatattct acaaggtgca
    42661 tattttacag aattagaata aataaattta aaaaaacttc ttacttatgt tgacataatt
    42721 tagtttatag gattataata tgcttaaata agcttattat taacttaaat taagcaaagt
    42781 taagcaggaa catgcaatgc tagcttgcaa tgtgactgta aagggggaaa aggtatgagt
    42841 aaggtaaata tatttgaagt tggtcctaga gatggatttc aaaacctgaa agagttttta
    42901 gaaactgata aaaagcttaa gattattgat ggtcttattg cttcagggat taaacaactt
    42961 gaaattagtt catttgtaag cccaaaagca atacctcaga tgaaagatgc aaaagaagta
    43021 gcaacttatt gtgtagaaaa atatcctgat gtgaaacttt atgcattagt tccaaattta
    43081 tacggtgcta aagttgcatg ggagtgtggt ataagagata tatcctatgt tatatctttg
    43141 agtgaaactc ataataaaaa aaatataaat cgtacccatg aagagtctat tgatgagctt
    43201 aaaaaaataa tgaatactta tccagacatg aatatatgta ttggaatggc tactgtattt
    43261 ggatgtcctt ttgaaggttt accaaaaatt gaagatgcca taagatttgc taaacgcctt
    43321 tgggaaaacg gtatcaggga gatgaatcta gcagatacaa tagggattgc tgaccctaag
    43381 caagtaagag aaactattag tgcattagtt gaagctttac cagagtgcag atggcaggta
    43441 catattcatg atactagaaa tatgggaatg gttaatactc ttgtggctat tgaatctgga
    43501 attactactg ttcagtctac acttggagga ttaggaggat gcccatttgc tcctggagca
    43561 tctggcaata ctgcaactga agatttagta tatatgcttg aacgtatggg gtatgataca
    43621 ggagttgatt ttgttaaact tcttgaatta tcaaagtatc agaagagttt tgtaaatgga
    43681 gtatatagtg gtcatcatgt aaatataaag tctgatagtt gtaattgtta gttctattga
    43741 ttaatcgatt aaaaaaatat gttgattaaa aaaatatgct gattggtaaa atagatttat
    43801 tgataaaaaa tataaattat aaaatacatt atctaataaa acaaataaaa aaattgaggg
    43861 ggattagtat ggatattata gctttacttt tatttttagg agttattatt attgcatttg
    43921 tacgtaagaa taatgcaggt attttggctc tagctattgg tgtcatagct gttagaattt
    43981 ttggaatgga tgataaagaa ctttatggag ctataagcaa ctcaatgttt actacacttg
    44041 ttggtataac actactcttt gcaatagtga actcaacagg agcattaaag cttgttgcag
    44101 aaaaaattgt agctgcttct ggtaaacgtg tttggcttat acctatagca gtttttatag
    44161 caggatttgt tgttgctggt gttggacctg gggcaattcc agcacttgca attatcccag
    44221 caatggctgt tcctgtagcc ataacggttg gttataatcc tgtaatgctt gcacttatag
    44281 gtgaatgtgg attaatggca ggtcgtatga ctcctattac tccagaaggt caaattataa
    44341 caaccgttgc agaaggggtt ggaattgaaa atgttatgcc tactatctta gcctgtcaaa
    44401 ctctttctac aattgtattt tcaatagtat tgtttataat tttcaaagga tataaactta
    44461 aaaaacctat aaatgttata gatagtaagc aaatagaaaa attcaatata aagcagataa
    44521 tttctctaat aagcatagta gttatgattg cttttatcat aatttttgat atgaatgttg
    44581 ggcttgcagc ttttgcagtt tcagctgtac ttattttttt caatattgct gatgaaggag
    44641 aatgtatcaa atcaattcca tggagcacga tagtaatggt tcttggagta ggtgcaatga
    44701 tgacaatcgt agatgcagca ggaggtattg acttgatgag taatgcatta tcatcactga
    44761 tgaatagtaa aacagctact ccaattatga gtatttcagc aggacttatg tctatggtta
    44821 gttctgcttt agcagtagtt tatccaacta tgatgcctat gtgtgttgat atagctaagg
    44881 cagttggagg agtagaccca ctagcactta ttgctgcagt tggtgttgga ggttctcttg
    44941 ctggtgtaag cccactttct actggtgggg cattgatttt agcagctatg ggttcaagta
    45001 agaaagactt taataaggaa gaacaaaata aggtttttgt acagcttttc attatgagtg
    45061 cagttggctt attggttatt gcagtagtat caatattatc atttaatgca ataacacatt
    45121 taatgaacta attagaataa ctgaaaataa atcattaaat ttaactttta atattaatca
    45181 atataatata atttggaggg gaagttatga aaaaaggagc attaagcaat ataaaggtac
    45241 ttgaccttac aagagttctt gcaggtccat attgtacaat gatgttggcg gattttggag
    45301 ctgatgttat aaaaatagag atgccaagaa aaggtgatga tacaagaggt tttggacctt
    45361 ttgtaaatgg agagagttta tactatgcaa atataaatag aaataaaaaa ggtatcactc
    45421 taaaccttaa atcagaagaa ggaaaaaata tatttttaga aatggtaaaa catgctgatg
    45481 ttgtagtaga aaattacaga ccaggtgtaa tggaaaagtt aggtctgggt tatgatgagt
    45541 tggctaaagt taacaatcaa ataatttatg gagctgtatc aggatttgga tgttatggac
    45601 catattcaga acgtccaggt tatgatatta ttgcacaggc aatgggtggt cttatgagta
    45661 taacagggca agaaggagga gaaccaacga gagttggtaa tgctatgggt gatgtactag
    45721 gtggaatgaa tttaactatt ggtatactca cagcactcaa cgctagaaaa ataataggaa
    45781 aaggacaaag agtagatgtt tccctcgttg attctgttgt atcgtctctc gaaactggta
    45841 tacagagata ctttgattca ggacaacctg aacttatggg taatagatat gcttcagcct
    45901 atccttatga ctcttttaag gcaagtgatg gaagttttgt aataggctgt ggtaatgaca
    45961 aactttttga aaagttatgt aaaaaagttc tttttagaga agacctttta atagatgaga
    46021 gatttaaaac aaatcttaat cgttgtgaac attacaaggc tcttaaacca gaaatagaaa
    46081 aatggtctat taagcataca ataaaagaag cagttgactt aatatcagag gctggagttc
    46141 ctgctgctcc tatctataat cttgattgtg ttacaacaga tgaacatata gcaaaagcaa
    46201 gagaaatgat tgtagatatt agtcatccta ttataggaaa tatgaaagtc aatggaaatc
    46261 cagttaagtt gatgacaaca aaagctaaaa ttgatatgcc agcaccatct ctaggggaaa
    46321 gtaacgagga gatttataat ggttggcttg gttttgatag tgataaaata aaagatttaa
    46381 aagaaaatgg agttatttga ttaaaaaata gctaaacaca cttgtgactt tagtcagggg
    46441 aggttcagtt agaataatat caaaaaatat taaatcaata ctgtaaatca aatatagaat
    46501 tgtcagaagc atttaagatg ggaaaagcta cttagacatt gagtctttgt ggcttttcct
    46561 attttcatta agaattttat attatcagaa tagatatgta ctaaactaaa gtattaacaa
    46621 tttaaattaa tagaaaataa actacctaat tatgttgaat aaagactact ttagtgaaaa
    46681 taaacttata gagatgctat ttaaattatt ttaatggaaa tatcaaaaat gagactgcat
    46741 tctcaaaaat gagactgcgt tttttaagta ttttctcgaa aatgagacaa aaatattgtt
    46801 ttatgggttt tattgaagaa aaattcttct gtgaattaca atttaatcaa ttttaaagtt
    46861 gaaaattatt aaaaatttag tgaaatttat gtatatttcg tttattttat taaaaataac
    46921 aattttttac taaaaataaa gaacttcaat ggtgattggc atagtacttg ctattttata
    46981 ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat ggaatctgta
    47041 atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt tattactata
    47101 aatagaccta aagctttaaa tgctcttaat agtgacactc ttagggaact atcacaagtt
    47161 attgatgtag tatcagaaaa tgaggcaata ttaggagtta ttattagtgg agaaggtaaa
    47221 gtttttgtag ccggtgctga tatacgccag atgcaaaact ataaaagtga ggaaggcaga
    47281 aaatatgcag gttatgctca gggagttttt gataaaattg aagctcttga aaaaacagta
    47341 atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat gagttgtgat
    47401 attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt aggagttatt
    47461 ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat agctaaagag
    47521 ttaattttta cagggagaca agtaaatgca gaagaagcta aatctattgg gcttattaat
    47581 aaagtggttc caagtgattt acttttagaa gagtctatga agatgatgaa ccaaatagtt
    47641 gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaagg gatagatatg
    47701 gatttaaaga atgctcttga actagagaaa gatattgcag gtttaacttt tgctacaaga
    47761 gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt tgaaaataag
    47821 tagctattat aactaaaatt ggaacaatgt agaatatatt caaaatctaa atttcgtgaa
    47881 aaaatacata tagtttaatt ttaaaaggag ttaatttatg aatacgggag taattataag
    47941 tttagtaggg attatcatag ctctatctct tctagttgta atggttatga agggaataaa
    48001 tatttttatt atagcaattg tatgttcatc attagttgct ataacaggaa gcctaaatgt
    48061 gtatgatgca cttaaagtgg attatatgac agggtttgtt ggatttcttc aggcaaattt
    48121 ctttattttc ctaacaggga cattaatggg aaaaataatg gagattactg gtggagcaaa
    48181 atctattgct aagatgattg taagatggat tgggaaggat aaagcactac tttctattcc
    48241 aatagcttgt ggaatattag cctatggtgg agtaagtgtt ttcgttgtaa gttttgcagt
    48301 gtttccgata gctttagaaa tttttaaaga agctgatttg ccacgaagat ttataccagc
    48361 agctttgact tttggatgct caacttttgc tatggttgca ccaggggcac cacaaattca
    48421 gaatattgtt ccagcatcaa cattagggac agatattatg gctggagcag taaatggttt
    48481 tatatcatgt gcagtaatgt ttatagttgg aagtacaatt ttgtacaaaa tggtttcaaa
    48541 agaaaaagcc aatggtggtc attttatagc acatgaatca gatatatttg atgaagcaga
    48601 agcaactaat tctaagagag aaaatggtcc caatggtgta attgccttaa ttccattgat
    48661 agtgtcaatt ttaataatta atgtaaaagt taatggaaaa gcgatagtac caattgaggt
    48721 gggagtattt attggagctt tattagtata tttattgtta aataaatatc aagataatac
    48781 taaaatagtt ggacatgttg gtgatgcatg taaaactaca gtagtagcaa tatgtaatac
    48841 ttgtgctgta gttgcatttg gttcagtagt aaaatcagca gttggttttg acttcatagt
    48901 taatgctatg actaatattc ctggtcctcc tattgcaggg gctgcagttg gaactacagt
    48961 tattgcaggt atttgtgggt cagcttcagg tggactagga attgcagctc cacttcttgg
    49021 accagtattt ttagccaagg gtgtttccgc tgcagcatta actaggacta tggcaatttc
    49081 atcagcagca ttagattcac ttccacacaa tggatatatt gttactgtaa caaatggtct
    49141 ttgtaaagaa acacataagg atgcctatat gccaatattc tgggtaactg ttttaacccc
    49201 atttattgga accatagtag cagtcctact gtttacaatg ttcccgggat tgccataaga
    49261 gaaaagataa aaggtataaa ttatattttg atgttataaa atcaattttc aaaatatata
    49321 caataaattt catgttttaa tactgtaaat atgtatttaa aataacaaat tgaccatata
    49381 aggaggaatt gaaagtgtta aataaaccat tagagggagt taaggttatt gacctaacgt
    49441 attttgtagc aggtccaggg acttctaaaa tattagctga ctggggtgct gacgttatta
    49501 aggtagagcc aagctttgga gacccaggaa gaaagacagg ggcaacaatg acaatgccaa
    49561 tagatgatta taataatcct ttttacagca catataattc aaataaaaga ggtttgtcta
    49621 taaatcttaa gagtgaaact ggaattgaaa ttatggataa gcttttaagc gaagcaaacg
    49681 tatttgtttc aagttataga acaggagcat taaaaagact aggtcttgat tatgaatcat
    49741 taagtaaaaa gcatccacac cttatctggg gtcagataaa tggttttggc gattttggac
    49801 ctgctaagga taatgcagga tttgatacag ttgccttttg ggcaagaagt ggggctatgc
    49861 ttgatatagc agaaaaagat acaagtccaa taaatccacc tatagccttt ggagatgcaa
    49921 caacttcttg tagtttatct ggaggaatat gtgcagcttt gtaccagcaa gctaagactg
    49981 gaaaaggaca aaaagtaatg gcatctttat ttgggcaagc aatatggaat gcatcatctt
    50041 tattagcatc tacacagttt agtgatgagt atccaaaaac tagaaaaaat gctatttcac
    50101 cagtaataaa ttcttatcgt tgtaaggatg gaaagtggat attcttaagt attttagagc
    50161 atgaaagata ttttgaggca ctttgtagat tatttggaag agaagactta ataaaagaag
    50221 agaaatttgc atcttcagta gaaagtaaga aaaatgctac agaacttatt aatattttag
    50281 atggtgaatt tgcaaagttt actcaaaatg agatggttga aaaacttact gctgctgata
    50341 tagctcatga aaaaattcaa catgttaatg atgtagtgac tgatgaacag gctattgcta
    50401 ataactatat atatgaacat acaagtaaag atggaaataa gacaatgctt gcatctactc
    50461 cagttaaatt tggaaatatt gaagttaaca tgacttgtga tgctccactt ataggagaac
    50521 attcagatga gatattgaga gaacttggat acagtgattc tgatatagag gctctaattg
    50581 aatctggaat tgtaacaatg aataaagaag catgtgaaat atgttaataa acatgtgaag
    50641 tatgttaata aataggaggg aattttatgg attttagatt aacagaagca caattaatgc
    50701 ttcaaagagt agcaaaagaa tttgcagaga atgaaatagc accaatagct gctgaaacag
    50761 ataaaacagg tatttttcca agggagcttt ttagcaaaat ggctaaaata ggatttaatg
    50821 gaattggaac acctgtagag tatagtggtt ctggtggggc agatatagaa aaggtaattg
    50881 ttgttactga gattgccaaa aaatgtgcag catctgcagc aatattatca atacacacaa
    50941 tatatgctca agctattttg aaatttggta ctgaggaaca aaagaaaaaa taccttccaa
    51001 tgatggctga aggtgggtgt gtaggtgctt ttgccttaac cgaacctaat gctggttcag
    51061 atgctgcgag agctgcaaca actgcaatta ttgatgaaga aactgatgaa tatgttttaa
    51121 atggaactaa gtgttttatt tctggtggag gtcaagcaga atcattaata atatttgcat
    51181 tgacagaccc atcaaaggga ataaagggaa tgtctgctat cattgtcgac aaaggtacgc
    51241 caggtttttc aataggaaaa attgaagaaa aaatgggtat tcatggttca gaaactgcag
    51301 agttaatatt tgataactgt agagttccaa agtctaactt attaggaaaa gaaggtaaag
    51361 gatttaatat agctatgact tgtttagatg gagcaagaat tggagttgga gctcaagctg
    51421 taggaattgc tgaaggggct ttagaagaaa gtatcaaata ttctaaagaa agagtccagt
    51481 ttggcaagcc aatttcagct ttgcaaggta ttcaatggta tatagcagat atggcgacaa
    51541 tggtggaatc agcaaaatta ttagtatatt atgctgcaga tttgaaagct agaggagaaa
    51601 aacacactaa agaagctgct atggcaaaat acaatgcgtc acgtactgca agagaggtta
    51661 caaatttagc ccttcaaatc catggtggat atggatatat gaaggattat ccattagaga
    51721 gaatgtacag agatgccaaa ataacagaaa tttacgaagg tacttcagaa atacacaaag
    51781 ttgttatttc aagagcagta ctaggatagg aggaaaagaa tgaatatagt tgtttgctta
    51841 aagcaagttc cagatactaa tgaagttaaa ataaataaag agacaggtac tctaataaga
    51901 gatggtgtac caagtataat caatccagat gatagaaatg ctttagaaga agctctaaaa
    51961 atgaaagatg aactaggagc agtgattaag gttataagca tgggacctcc tcaagctaaa
    52021 agtgctttaa aagaagcttt ggcaatggga gctgatgaag cttatttaat ctcagataga
    52081 gcttttggtg gttcagatac ttgggctaca tctacaatta ttgctgctgc tatagagaag
    52141 gttggaaagt atgatgtaat attctgcgga agacaagcta tagatggaga tacagctcag
    52201 gttggaccag aggttgcaga atttttaggg ataccacaag ttacttatgc gaaagaagta
    52261 aaggttcaag atgacaaatt actagtaact cgttatacag agacagggga ttatttaata
    52321 gaagcaaaga tgccagtttt attaactgct ataaaagagt taaacaatcc aagatatcca
    52381 agtgtcaagg gaatcttaga agcttataat aatggagatg caaagattac tgtattaaca
    52441 cttgcagatt tagatgttga tacaactcaa attggattaa agggttcacc tacaaacgtt
    52501 tataagtctt ttgtaccagt taaagacaag cataatgaaa taatagaagg tataaataaa
    52561 aaagaaaaag ctgagaaact aatagaaata ctattcgatt tgaaactagt ataggagtgt
    52621 taagatgatg agagcaaaag ttaatcaagg tataaattta aatgattata atggtgtttg
    52681 ggtaatagga gagcaaaggg aaggtaaaat taatcctgtt acgatagaac ttattggtga
    52741 aggaagaaag cttgctgacc aattaggaaa agagcttgca gttgtgatag ctggatatga
    52801 agtagagaaa gaagtgaagg aattacttca ctatagtgtt gataaagtat attacatcaa
    52861 tgaccctctt cttaaagatt tcactactga tggatatgca atttcaattg caaatttaat
    52921 cgaaagaaaa aagccagagg ttgtattggt tggtgctact tctataggaa gagatatagc
    52981 gcctagaatt gcaggtaagg ttggaacagg tctaactgct gactgtacaa aacttgaaat
    53041 agattcaaca gataataagt tattacaaac aagaccagca tttggtggaa atttaatggc
    53101 cactattgtt tgtccaaaaa atagaccaca gatgtcaaca gttcgtccag gagttatggc
    53161 aaaagctgta agaaacgaat cagagactgg aattttagaa gttgttactc cagaacttac
    53221 tgaaaaaatg attagaacta gattagtaga gatacttcca caagaaaaga aatctgtaaa
    53281 tctaactgat gctagaataa tagtatcagg aggaagagga ttaaaaagag cagaaggatt
    53341 tgagcttata aaagaattgg ctgataaatt gggggcagag attggtgctt caagagcagc
    53401 agtagattca gggtggatag aacattctca tcaagtggga caaacaggaa ctactgttag
    53461 accagaacta tacattgcat gtggaatatc tggagcaata caacatttag caggtatgag
    53521 tgattctaaa tatatagttg ctataaataa agatgcaaaa gcccctattt ttagtatatg
    53581 tgactatgga atagtaggag atttatatga aattatacct gaaatgatag aatcattaaa
    53641 taggtaactt ttagaaaaca ttgaatatgt agaatgataa gtcttataat attgtattgt
    53701 aagacttatt atttttgctg aaggttatag tggggagatg aaacaatgaa agagcagtgg
    53761 tataaggata tatttgcaag agtattatct atgactgatg atggttttat tgttgtaaat
    53821 agaagtggag taataataga tattaatgat aaatattgtg actttttagg aaaagaaaga
    53881 aaagatataa taggacaaaa tatacaaagt attataccaa atacaaaaat gctagatgtt
    53941 atgaaaaata agtattgtga agaaggagct atacatcatt attcaggtgg aaatacaaaa
    54001 gaaaaaagtg taatagtaag taggtcttat gtggaaaatg acaatggaga agtagttgca
    54061 ggagtagctc aagttaaatt tagattgcaa tcatttgatg tagctaaaaa gttaatgtca
    54121 gaatatatgg agctccaata ttataaagag cagtttaagg ataactgtgg ttttgacaag
    54181 ctcataggtg aaaatagaga cttcatagaa cttaagaaaa caggtgtgaa agcttctaaa
    54241 acaaactttc cagtactttt aacgggtgaa acaggtacag gaaaagaagt ctttgctaga
    54301 gcaattcaca ataatagcag taggtcagat aaacctatgg ttagtataaa ctgcgctgca
    54361 ataccagaag aattattaga gtcagagcta tttggctatg acgaaggagc ttttacagga
    54421 gcaaaaaagg gtggtaaaaa aggtaaattt ttagttgcga ataatggtac aatcttttta
    54481 gacgagatag gtgacatgcc tttgaccatg caagcaaaac ttttaagagt tcttcaggaa
    54541 agtgagatag aaccagtggg aggtcttaaa actataaaga tagatgtaag agtaatctct
    54601 gccacaagaa agaatttaag taaaatggtt gaagagggat tatttagaga agacctatac
    54661 tatagattaa atgtaataaa cattcatatg atggaattga aggacaggca agatgatatt
    54721 ttattgctag caaattatat attaaacaaa ttaaatgttg aatacaagga attgaaggtt
    54781 ttaagtgata aggttaaaaa ttgttttatt aactatactt ggccgggaaa tataagagaa
    54841 ctacaaaatg taataaaaag tgcctatgct gtaagtgatg atatggtcat tatgatgtgt
    54901 gaccttccat ctaagatgga taatatcagc agagtagctc aatgtaatgt ggatagcaat
    54961 tgttcaattc atgaaatggt tgagaattat gaaaagagct taattattga tgtgttaagg
    55021 aagtataatt ggaaatgttc taaggcagca gaggttatgg gaatacacaa gagtctttta
    55081 tataagaaaa taaaaaagta tgaaattgaa ttaaataatt aagataaatt gaattaaggc
    55141 aatgctaaaa aaacgccctt attcaaagcg aactgtataa actgttttat gcagttaaaa
    55201 cgtgtaaaag tagatgtgaa gtactaatgt caatcattag tactttttta ttatatttat
    55261 aattactatc tatatttcag tatcttttga tatctaagat tattatgttt aatttaggat
    55321 ataacaaaac cctcttagtt atggacagtt ttttatattt taactgtcta ctaagagggg
    55381 atagtttagt ttaaatttag agtaaatttt atctagcatt tataaaaaat attttgaata
    55441 gcttttttgt tatttaatca taattttcta cttctttatc aagttctttg ccacaagaat
    55501 cacacttata tatagcattg tatccgtaat catattcatc aactattttc ttttgtctaa
    55561 atacaccacc acactcacat aggaaaccaa tttttataag tgtaacgata aattgtgatt
    55621 ttataaattt tatatcatta catttatcgt tagtgtaaaa tgtataaata taagagttat
    55681 cgcactcatc tcttaattca aatctttcaa tatatgtgtt attttttaat tcctcaaatt
    55741 cttctttagt taaatatgat ttttgttgca attcatgtaa acgcttaatt tggtccatta
    55801 tttatatcct cccaataatt ttagtatttt aagcttagat aaggaatcta agttttatta
    55861 aatttatccc aaggaattat ttaaatcata tttagtttta ttaattacaa catatataaa
    55921 ttacacgaaa ataaaactaa ttgtaaaaat aatatcctgt gaaaacctaa actactaacc
    55981 ccttactaac ccgaaaacta aaaaaagaaa atatatccaa atataagtga tatataaata
    56041 aagatgtttc atataaattt aactttaaat attctgacaa
//
LOCUS       DAMJVI010000012        53616 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.012, whole genome shotgun sequence.
ACCESSION   DAMJVI010000012 DAMJVI010000000
VERSION     DAMJVI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.012"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            333..731
                     /locus_tag="PC545_000825"
     CDS             333..731
                     /locus_tag="PC545_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2455615.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     ILMSIADYFNVTTDWLLYGKEKVNVNSSVKEDKEDYLHVTNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(948..1157)
                     /locus_tag="PC545_000826"
     CDS             complement(948..1157)
                     /locus_tag="PC545_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455616.1"
                     /translation="MCKLDSDGVIECCRAIDDFITALSNIKSLNMERLNTLTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1189..1398)
                     /locus_tag="PC545_000827"
     CDS             complement(1189..1398)
                     /locus_tag="PC545_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455617.1"
                     /translation="MDARKKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1697..2116
                     /locus_tag="PC545_000828"
     CDS             1697..2116
                     /locus_tag="PC545_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455618.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSIAEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKEKDLGKIEYNKNKDEKIDK"
     gene            2344..2799
                     /locus_tag="PC545_000829"
     CDS             2344..2799
                     /locus_tag="PC545_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455619.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(3006..3266)
                     /locus_tag="PC545_000830"
     CDS             complement(3006..3266)
                     /locus_tag="PC545_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455620.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(3529..3882)
                     /locus_tag="PC545_000831"
     CDS             complement(3529..3882)
                     /locus_tag="PC545_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455621.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQYKDISDISIGQVKQDDDITNNFI
                     ANVEIYEMLLNQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(3899..9220)
                     /locus_tag="PC545_000832"
     CDS             complement(3899..9220)
                     /locus_tag="PC545_000832"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012632.1,HMM:NF024930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455622.1"
                     /translation="MKRTKLLQRGNFFGDKNMVVDEFDEGYDNYDFINFFTGCCNYTF
                     GLKNNNILYGCGDNSNFQLGLGEDNTTRKLFTKIPNISTNIKKVACGESHAVILTSDG
                     ELLVAGINTDGQMGLGLEKVGKTVSTFEKVPEIKGVKDIACGLQSTYLLYNDGTLYVA
                     GNNLYGQLGLGTNGASANVNTFTKVDVDNVKAVFSYNKSAFIIKNDNKCYSTGFNNQG
                     QLGLGDKNNRDLFSLVSINDVKTIACGSEHTVLMTYNNDIYGCGKEKCFGNALQSSLF
                     TKIEEVNIKTIACGHGNTMLIDNKGTLKVAGNNDIYQLGIANYSENIDNSFIDLKNIV
                     AKNIFIGLSHSILIDSNNDSYCTGDNTYGQLGSFFDDMHIVEFKKMDSEKYSYSNYIN
                     LIKSEDKLTLLKEEMEIKDIELPLDIHSVRDVVFSPYCTLVILGNGDVYGLGNNRYKG
                     MGSDLPSQLNELTKLSISNVKSIVASKNISGGIFYIKNDDTCYYSGPNSNSIAGVLPS
                     NSDVFKKISIDNVKKVVINTDLSNWFSLIVTNNKQIYTSGKSSSYVNGLSNALISQYT
                     EISLSNVTDAYSSYNATFIVVDEKKVYATGINTNYLLGFSTSDGSNVNLGLLSDWYYI
                     NISGSSYSRVSCTNNITKINNIIIYEYVTVFCTNIGSFLTGYHGTSWTKPTDSSYRVQ
                     YQGISYAGYLDSYIYNYYPTRCTQSSSSTTFAYLYNGESSSNLKNVNPDNLLISGGSS
                     YIHQYGRNYLNNQSSNNIAASNINSGPITSDKAIFLYKALLYLSSNTLYGFGNISESA
                     KELDVSDTQDGYNATNYKKVMKNIKNIFIPPYDLSRDKTRFAILTDKSLFICGYNSKG
                     THGISVNSSLNLNNKINYNKKNSSSEISSNIQEIYSHSKSTYLLTNNNMLYSVGLNDV
                     GQLGVGDEINRKVFTKINIDNIKSINVNRFTDNSKHAFAIKNDNTCYAVGLNNSGQLG
                     IGDNVNRNIFTKINVENVKYVAVYGNTSLLLTNDGLLYGAGNNGKGQLGLGDTTSRNI
                     FTRIPINGVRDVYLCNDVSIIVKNDNTCYVCGLVNGYFGFTEGSISTFTKINIENVKS
                     VVTAGSEATFFITNDNMIYTTGKKERVFFSTETNDIKGIRVINNIINAKKIVVNGYTS
                     AILTNDNKLFVGGLSGYGSIANNNNTNSVEDVKDVFVTANNTLYIDNNNNLISSGRDT
                     YGISDESYRDMSVPYYKVSIKKDVDTVFSSYNTIFIKDIYGKFYSSTGDNRYNHLGIH
                     HRYDNDKNEALEGSLHSYFKTDNTSDKIVFNKKNEKLVMFNDKYIKTNNKYINYKNIF
                     KDNFKYTSIILPFEVSDIDISKTHSLAVAKDGKLYGIGSNSYKEINQTLEDIELLTLT
                     EVNISDVKKVACGDNYSYIIKTDNTLWSYGKNTEYQLGVGHNNDVRELQKVTGLPSVK
                     DISIYNSMTLVLTNEGELYAQGYNTNGLFGLGESEKDKIIRTFTKVLTNVKEIKSHND
                     DHILVIKNDNSLWITGKNKSMYKISISITDLYEFTKIPIPEHLNDILDIELSDDTIYM
                     ITKVDTSKASIEIVEKSISQVRVVVQDPNNVIEKLEMFINDELISTKTNLEINSIIFE
                     IPQNKIVLGENKILIKASSPTGDLYSSMFIFKSETGLKVKKDSILMINNKVYSIINIT
                     ENNTDLIVTLNEGLKDDMMENNPIYQLINKTKVQVKINKSDLFKDMKLVEIKKSDSSY
                     QEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(9237..10220)
                     /locus_tag="PC545_000833"
     CDS             complement(9237..10220)
                     /locus_tag="PC545_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2455623.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVKIGEELLSTEAKNLSGAINEVVEKIKNISIDDVIGGQIQTELSVLKNSYN
                     KLSEKVLDILIYLELESEIDVDEAGYWYDTLTNAKNIIAIEGLKLDLNRKCITGELGS
                     VTFKNVVLPFNANRVRYIHEMDNNFVETKSNRAYSIGQTDITLNKYSYEIR"
     gene            complement(10221..10919)
                     /locus_tag="PC545_000834"
     CDS             complement(10221..10919)
                     /locus_tag="PC545_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDF2455624.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSMVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMISREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(10916..11968)
                     /locus_tag="PC545_000835"
     CDS             complement(10916..11968)
                     /locus_tag="PC545_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2455625.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMVSGNNLELSKI
                     YLELSKMHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGEKGTVINNGTII
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKKYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEISISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTTGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(11961..12389)
                     /locus_tag="PC545_000836"
     CDS             complement(11961..12389)
                     /locus_tag="PC545_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2455626.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVNVKSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(12389..12715)
                     /locus_tag="PC545_000837"
     CDS             complement(12389..12715)
                     /locus_tag="PC545_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 family protein"
                     /protein_id="HDF2455627.1"
                     /translation="MANPINEFIGIIREEGKYHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMIDRQLETFDTETNQEHQHEVKNPFIDNFESGDMVIMFRIGEKFAVV
                     SKLVSL"
     gene            complement(12731..14260)
                     /locus_tag="PC545_000838"
     CDS             complement(12731..14260)
                     /locus_tag="PC545_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2455628.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLTQSEVSYNFKDKLVEDIAK
                     QVFAENRLSVGIIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEDYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRRDFNTARRV
                     L"
     gene            complement(14542..14964)
                     /locus_tag="PC545_000839"
     CDS             complement(14542..14964)
                     /locus_tag="PC545_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455629.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKAAVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(14964..17417)
                     /locus_tag="PC545_000840"
     CDS             complement(14964..17417)
                     /locus_tag="PC545_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455630.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASNRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNEFNSNIMIILIFKAKSDEVEKTKNKLLGNLKKIGGK
                     IWNIVIKAKDMTKRVISSILGKLKQVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASGIISKVFPKLRLFAGKVWSGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQIDVDDYYGSLVRMANDTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSGKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KITSKLVASIQLAQLGGVL"
     gene            complement(17417..17584)
                     /locus_tag="PC545_000841"
     CDS             complement(17417..17584)
                     /locus_tag="PC545_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455631.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(17611..18057)
                     /locus_tag="PC545_000842"
     CDS             complement(17611..18057)
                     /locus_tag="PC545_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2455632.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(18171..18599)
                     /locus_tag="PC545_000843"
     CDS             complement(18171..18599)
                     /locus_tag="PC545_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2455633.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(18613..19677)
                     /locus_tag="PC545_000844"
     CDS             complement(18613..19677)
                     /locus_tag="PC545_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2455634.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAVLGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(19682..20125)
                     /locus_tag="PC545_000845"
     CDS             complement(19682..20125)
                     /locus_tag="PC545_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455635.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEGSCFFVSILS
                     VICTPVMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYVLNFTFTLNYLDSVYEEDVVYENMKEINLNLGE"
     gene            complement(20374..20814)
                     /locus_tag="PC545_000846"
     CDS             complement(20374..20814)
                     /locus_tag="PC545_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455636.1"
                     /translation="MENKKDILFKETDKRLHNYKYLDIKIKNINLDIKRCENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKMEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIFKLSEIL"
     gene            complement(20919..21116)
                     /locus_tag="PC545_000847"
     CDS             complement(20919..21116)
                     /locus_tag="PC545_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455637.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            21285..21605
                     /locus_tag="PC545_000848"
     CDS             21285..21605
                     /locus_tag="PC545_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2455638.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFNVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            21687..22193
                     /locus_tag="PC545_000849"
     CDS             21687..22193
                     /locus_tag="PC545_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455639.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGIKIISLL
                     SNLKQNSKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIINIINSGITCVYEMADILNVDITFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            22894..23649
                     /locus_tag="PC545_000850"
     CDS             22894..23649
                     /locus_tag="PC545_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2455640.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(23792..24220)
                     /locus_tag="PC545_000851"
     CDS             complement(23792..24220)
                     /locus_tag="PC545_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDF2455641.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(24641..24841)
                     /locus_tag="PC545_000852"
     CDS             complement(24641..24841)
                     /locus_tag="PC545_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2455642.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(25024..26193)
                     /locus_tag="PC545_000853"
     CDS             complement(25024..26193)
                     /locus_tag="PC545_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HDF2455643.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANVEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
     gene            26429..27250
                     /locus_tag="PC545_000854"
     CDS             26429..27250
                     /locus_tag="PC545_000854"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HDF2455644.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVNFDSIYSGFLGSKHQIEIVANFINDYPNAFI
                     VVDPVMGDNGVMYPIFTEEMRHEIKELVKHSDLTTPNLTEACFLTGNDYTKSDYNRDE
                     LIYIAKSVSDLGPSKVVITGILEDDNILNLAYDRDNDHVFFTSVKYNNCSYSGTGDIF
                     TSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFENFLSDLTNI"
     gene            complement(27326..28714)
                     /locus_tag="PC545_000855"
     CDS             complement(27326..28714)
                     /locus_tag="PC545_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase"
                     /protein_id="HDF2455645.1"
                     /translation="MLGGKKSNNKKVEKLRTIFIKYLSLFFIMTISIVLLLMLSFSVL
                     LSSGVILPANYAEKQFNKYKEQIISSEKVTEDIIPSIYEYGVYTLDGSLISGTFNKKE
                     SKEVWNLMRDIEERHAYSESYIKFFKKDEVFIIKYKIVSEYSSPILRAYLPKPETLGM
                     IIFSIIFFIEIVILSKVFGKKFNIEMELLKNTTEKIEQQDLDFVVESSKIYEINNVLF
                     SMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPELL
                     EYMEYISKGVTQIEKYINTLIDISKTENGYILNKEAISMSEFIKDVLVQIEALASTKN
                     LDVEFYKQDNLPKNITIDRELLFRAIMNIISNAIDYSPSQSKLYISVSVRNKYLKFVV
                     TDCGSGFSKADLSKATEQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILYIENSVKT
                     GGAMVTIEIPIV"
     gene            complement(28849..29607)
                     /locus_tag="PC545_000856"
     CDS             complement(28849..29607)
                     /locus_tag="PC545_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HDF2455646.1"
                     /translation="MRQLIRIFCSDLIKLKRNFIILMHICIALMGMGLFLWYYKVSGA
                     DNILKIVAYLQVIAIAFPLLSGVVCSMFVEQEYYAGNYKHMLTTSNPKYLTLISKYII
                     LVCLGFVATLVSVLGFKFGISKAYFASSFYMISILILIGCNLFEYILHLFLSLRFGKG
                     TSIGVGIVEMLLSALLLTGLGSGIWQYFPCAWGVRFITIWSSFSSSKTVEYIKVESIK
                     AYQSTGLMCGFVTILAFVILCIWFSKWEGKKSEE"
     gene            complement(29609..30325)
                     /locus_tag="PC545_000857"
     CDS             complement(29609..30325)
                     /locus_tag="PC545_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HDF2455647.1"
                     /translation="MERFIRVEFQKMKGTFGMKLIWIVPVFCILLCGLGGGQNAAFNW
                     WYTTFLPGALTLISSLIVLKDEKSKYRGILPLPIEMKDVWTGKMIYCIICCFIASIIS
                     AIGVVFVGIIYKETISIGQNIIATILIILLFMWQIPLCMFLASKFGIFVTVLINMFFT
                     IVGVAVFSTTEMWYMFPYSIPSRLMAVVLHILPNGLPVPEGSEFLNNAVILPGVLISI
                     VLFVLFALATRVWFNNREVK"
     gene            complement(30327..31034)
                     /locus_tag="PC545_000858"
     CDS             complement(30327..31034)
                     /locus_tag="PC545_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2455648.1"
                     /translation="MSNYILQVKGVSKKFKEQMAVDNISLAIKSNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLETVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVMGYQGEVSKEQN
                     LEKLFMKVVAENRKRGQ"
     gene            complement(31150..31686)
                     /locus_tag="PC545_000859"
     CDS             complement(31150..31686)
                     /locus_tag="PC545_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2455649.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDED
                     AKEETTSKMINSKKTTKIPIEIISKDEKIVKYLQIDEESSLKDKLRLILDTLSNEYFN
                     GLDMEVEVKEKDNLVKINLIEPDKKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            complement(31788..32858)
                     /locus_tag="PC545_000860"
     CDS             complement(31788..32858)
                     /locus_tag="PC545_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDF2455650.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAEFKK
                     ENKLYQEKVNIDKLDIAVDNINVTIKKSSSEDVRVTTRGNNEFYKYNVTLKDKTLVVK
                     GERKYENKIKKIKNFDQLLNTSINSMFSHDYREIIIYVPNNVDINASSISSHLFVYDN
                     VASNTITYKTSYGGFSRAITENKVNRLENLNLISNNNLHLSTKSILGVKNVNIESESL
                     YISSENEDVFINNIEEYIPENVNIKEKIGRNSNYESEFYLYSDMPIAKFLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEAIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNSLEL"
     gene            complement(32851..33909)
                     /locus_tag="PC545_000861"
     CDS             complement(32851..33909)
                     /locus_tag="PC545_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDF2455651.1"
                     /translation="MNLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIIASLGSPKSIAKELILQTKEKNIEEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMNTDILNGKFSRFKSNVKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSATIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGLEILAWQILLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLEDIENKKGEDKHE"
     gene            complement(33896..34228)
                     /locus_tag="PC545_000862"
     CDS             complement(33896..34228)
                     /locus_tag="PC545_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2455652.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DEGGNELE"
     gene            34427..35548
                     /locus_tag="PC545_000863"
     CDS             34427..35548
                     /locus_tag="PC545_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HDF2455653.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKECIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYFKKSFDSRHDLSHKLYTPKLSEYIADI
                     NPDLIISTFPLAAACVNNFKIKNPDINIPTLTVITDVVDSMEWVFENTDLYFVPSPEI
                     KNRFFQKGINPDSIKVTGVPVDKRFQIESKELCCDKYRLLLLGGGRGLFDIDEDFMHW
                     IDEFIEEHSNSIEITIVTGKNKKLYDNLTHKKPLKNIKVLGFVNDMYNLIRECDLMLT
                     KPGGATIFEAIQSQTPVLVKMPKVGQEIENAKFIIDKGLGMIYSDDLDLKNIFYKLVS
                     NEFDSIINFMKKNLKEFKTVIHPEKIADYISELIDNHYS"
     gene            complement(35676..37574)
                     /locus_tag="PC545_000864"
     CDS             complement(35676..37574)
                     /locus_tag="PC545_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HDF2455654.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLEDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVNPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEAGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAIENKDYQTASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
     gene            37889..38665
                     /locus_tag="PC545_000865"
     CDS             37889..38665
                     /locus_tag="PC545_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2455655.1"
                     /translation="MEYIIFDLEFNQGFDKKLNKTVSNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKDIHPFISRMTNIKNSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFTLENVKQRKNK"
     gene            complement(38757..39509)
                     /gene="xth"
                     /locus_tag="PC545_000866"
     CDS             complement(38757..39509)
                     /gene="xth"
                     /locus_tag="PC545_000866"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDF2455656.1"
                     /translation="MKFISWNVNGIRACVGKGFLDFFKEVDADIFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLVSADIHTEILGSDHCPVELEIK"
     gene            complement(39670..40620)
                     /gene="galT"
                     /locus_tag="PC545_000867"
     CDS             complement(39670..40620)
                     /gene="galT"
                     /locus_tag="PC545_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDF2455657.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDEEC
                     PFCKGNEAHATDALFEIESEEGWLVKSVYNKFPIIDDMARDVYGVHEVMIESDKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLDRRVVYNGEAFLVLIPYATKYSGE
                     VRIIFKDKIEFGELNDNNIQELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEDLTKKLKID"
     gene            41005..42201
                     /locus_tag="PC545_000868"
     CDS             41005..42201
                     /locus_tag="PC545_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2455658.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYDMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
     gene            complement(42313..43653)
                     /locus_tag="PC545_000869"
     CDS             complement(42313..43653)
                     /locus_tag="PC545_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2455659.1"
                     /translation="MKKRYNIAIVGGGSTWTPGLLKSLCKMSDRFPLNKVTMLDVVEE
                     RQRLIGEFGKILFKEEYPKATLEYTTNPDEAFIDVDFVFVQMRTGGLKMRELDEKIPL
                     QFGLVGQETCGAGGFAYGLRSIGDMIEMVKTVRKYSPKAWILNYTNPAAIVAEALKRV
                     FPDDKRLLNMCDQPVNLLRSYGRLLDMDSRTFEPVYFGLNHFGWFTHLYDKNGEDLVP
                     KIKDLVANSGFQPVDAEQRDKSWLDTYGMVKDMLEDVPDYLPNTYVQYYLYPDKKVAK
                     SNINCTRAREVMNGREKRVFEECKSVIEKGTSIGSNLVHNDAHGEFIVEVAEAIAHNK
                     HQIAIVITENNGAISNLPDDAMVEVAAILTKNGPRPLHVGNIPTFYKGLLEGQLAYEK
                     LAVDAYFEQSYEKAILSLTLNRTIISPTKARQVLDALIKVNKDYWPTLYKENEK"
     gene            complement(43792..44544)
                     /locus_tag="PC545_000870"
     CDS             complement(43792..44544)
                     /locus_tag="PC545_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2455660.1"
                     /translation="MNLIDKLDINQKKLSTNEISLLNYFLKNSEKINQKKIKDIANET
                     FNSTAYIVRFCQKLGFSGYSEFKNFLVFSYSNRNERNKEFTKSQSDIFTDVMDTQNLI
                     NVENIDMCLNEIHNSKHIYFFGVGSSRLVCTEMSQRFSAIGVNVKYYDDSTLMYLAAS
                     NITKDDLVIAVSMSGETAQVIKACNIAKTNQVKVISLTNISVNSLSNIADIKLFVSSP
                     QYNFNSVNYTSRVPALSLMEYMFHKYIEKYNR"
     gene            complement(44692..46269)
                     /locus_tag="PC545_000871"
     CDS             complement(44692..46269)
                     /locus_tag="PC545_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2455661.1"
                     /translation="MKQKMKGFFLQLGKSFLLPIALISAAGIFLGISSAFSNPNIVSD
                     MPFLANNFIQGILGFTKAITGVLFGNLAIFFAISISVGLAKEEKAVSAFSGFVGYLVL
                     HTSVNYMLGCRGIIAEPDMMREMGQAVTLGIQTLELGVFGGIVVGIITASLHNKYYNI
                     KLPDYLGFFGGTRFVPIITTVVFSILGIIIPYIWPYVNTIIQSVGIGIASLGYLGTFV
                     FGFLERLLIPFGLHHILNAMFRFTEVGGSLVVGGQEVFGALNIFLAQLSDPETISFST
                     EATRFLAQGKIPIMVFGLPAAAYAMYKTAKPENRSKVKGILLAGALATFVTGITEPLE
                     FSFLFVSPILFVIHAFLSGISFMMMHIFGVAIGNTQGGIIDLVVFGVMQPNTKWIYSI
                     ILGLVYAIVYYNVFKILILRFNLQTPGREEITDINFDLKPLSNKVEIAQSGETTKRAL
                     SIIKYLGGKDNIEDITNCITRLRVVVKDMSKVDENAFKKYTGAMGVIKVSDKDIQIIY
                     GPSVSQVRDEVSFAMSI"
     gene            complement(46681..47133)
                     /locus_tag="PC545_000872"
     CDS             complement(46681..47133)
                     /locus_tag="PC545_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2455662.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(47444..48889)
                     /locus_tag="PC545_000873"
     CDS             complement(47444..48889)
                     /locus_tag="PC545_000873"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDF2455663.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYHSSYAVSGQTYTRKLDYQVISILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINRRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(49033..49941)
                     /locus_tag="PC545_000874"
     CDS             complement(49033..49941)
                     /locus_tag="PC545_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2455664.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKK"
     gene            50198..51022
                     /locus_tag="PC545_000875"
     CDS             50198..51022
                     /locus_tag="PC545_000875"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDF2455665.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKKPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            51062..51592
                     /gene="lepB"
                     /locus_tag="PC545_000876"
     CDS             51062..51592
                     /gene="lepB"
                     /locus_tag="PC545_000876"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2455666.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRVIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKLFAMGDNRENSNDSRFPDVGMVDEDEVLG
                     KVMVRLLPLDNIGKVD"
     gene            complement(51674..53455)
                     /locus_tag="PC545_000877"
     CDS             complement(51674..53455)
                     /locus_tag="PC545_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDF2455667.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINEDVKVVPKVTPFKGIN
                     TDLLYVKDGRILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLEEYLNDENDEINLNLFLLDIC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEDVQIKDVVSIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLVIFTHTKQLCNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKTFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT        17651 a         8782 c         5935 g        21248 t
ORIGIN      
        1 taatataaat tacaatataa tactattttt ctcttaatct ttttctcttt tacagacaat
       61 caaaaagcaa tcttagttta tcgaaataaa tttatacaat ctattcatac acccctttac
      121 atttatataa caattaaacc tattgttata tatacaaatc aaaataaacc atatattttc
      181 aactataata tgttaaaatc atattatgag catattaatt taagcaaaat gtcatttaga
      241 ctttaattta gtagttttat attataaatt tatatatttt taattttaaa tgtcatttag
      301 acattactca taaaaagaaa ggagtgtatt ttgtggatac tttaggaaaa agaattgctt
      361 atttgagaaa ctccaaaaaa cttactcagc gcaaacttat ggatatttta aaatttgaaa
      421 acttaggcaa gtatgaaaca ggagatagaa aacctaattg tgatatacta atgtcaatag
      481 ctgattattt taatgttacg actgactggc ttctctatgg taaagagaag gtaaatgtca
      541 atagtagtgt aaaagaagat aaagaagatt acttacatgt tactaatgat gaaatgacga
      601 tattaaatct atacagacaa ctaaacgaac gtgataaaat aaaaatagaa ggaatacttg
      661 aacttaagat atctgaatat aaagacttaa aaaagcattc ctctaataat aatgaagata
      721 aaatggttta aacatacata atttgatacg attttacatc atctattaac cttctttttt
      781 tgtagtatat tactaaaata tggatattgg attatttaaa aacaagtata gtctaatttc
      841 aagcacattt ataatattta gattatctta tccatatttt agtttaagta ctttgaaaaa
      901 tataatacat ctctatgtat aacttatatt cctaagaaat gcattactca tgtttttaat
      961 tcttccaaca tctttctata cacaattgta catccttcat aattcccctc tttaagaagg
     1021 attgaacatg tactagaata tttagttaaa gtatttaatc tttccatatt taagcttttt
     1081 atattactga gtgctgtaat aaaatcatca attgctctac aacactctat aaccccatct
     1141 gaatccaatt tacacacaag aatattcacc tactttatat actatttatt aatcatcttc
     1201 tgaatattct atattaagta attgataaat tgatgattta tattttttac ctgttcttct
     1261 tccatttaaa atagctgaca aatagttttc attaacacct attttcttcg ctaattccct
     1321 ttgagtcata tttaattcaa taagtctttg cttgacttgc actcccaaaa aaggtatcca
     1381 tttttttcga gcatccattt atcaacttcc tttctaagcg atttatattt acttttatat
     1441 tattttagct tacgcacccc ctctatttag tatatatttt taatatgtac acatattaag
     1501 tatataaatt tatttaatac taaataagat tttttgatta ctttgtgcta aaattaggat
     1561 gtaaattcgt atcattaagt taattataaa gtcattttga ccttaagtca atggttttta
     1621 tgtcattttg acctttaatt tgaaactatt tacatcttaa aaagtctttt tgacattaat
     1681 tgaatggaga gtgtttatgg atactttagg agaaagaatt gtttatttga gaaaagcaaa
     1741 aaaccttaaa caatatgaac tagaagaaat gttgggctgt gacaacttaa gtaaatttga
     1801 gagaaacatt agaaaaccaa attatgaaat attaaaatct atagctgaga tatttaatgt
     1861 ttcagtagac tggctattaa atggagataa tctatcacat aaatcagatt taatttgcga
     1921 ttcctcctca aactatccac ttaatagcat aaactctaat gaaataaagc ttttaaacaa
     1981 ttttagaaaa ttaaatgatt atgataaagc taaaatagaa ggtatgattg aattaaaact
     2041 tcatgaatat gaaaaagaga aggatttagg aaaaattgag tataacaaaa ataaagatga
     2101 gaaaatagat aaataatttt tatgttgctt ttgatacgat tttacatcat ttctttgttc
     2161 taatattttt atttcattta ttttcttttt tataatttta tgataaaata attagtatat
     2221 tttgctttac ttgaatgtaa ttatttatac aaaagtcaaa cataatataa atgtagtaaa
     2281 tttttagttt caagtaatat ctcgttatta taactaaata tttattaaaa ttaggaggat
     2341 aaaatgggct taagagacaa attcgcgcaa tcatttgcta gatcaaaaac tatgagtggc
     2401 cctgagaaaa aggctaatga aataatgggt aaattgcttc ttaagaaagc tatattacct
     2461 atcgttttaa tgttcgtgat aataatcgct ggagctatgc ttaaaatcaa ttcttgggtt
     2521 acacttggca taaatttagt tattgctgtt ggtgctttct tctacataag aaattctagt
     2581 aaaaagtatc agaactttaa accttatgtt ggcaatttga ttagtttaga gaaaaaaggt
     2641 aaaaaagagt acgttgctat tataaaacaa ggtaagttac cagttaagtt acaaatagct
     2701 tatggtggtg aagaccttga acatgttaag aaaaatcaaa tggttcaaat aagttataat
     2761 ccagatgcta aaatagctat tttggttaat agacaataat ttaataaaaa aaaggagttg
     2821 tcttaaaatt gattttttct aatcaaattt aagctcaact ccttttttaa tttttatatg
     2881 ttatttttat tacttttttt aattaatatt ttaaatatta aatccttctc aataaaatag
     2941 tgaactatct acaatactac tttatctttt cctaacaata tataatctgg tgctactctt
     3001 tcatgttata caccattttg taacatacct tttatatcag taacatcttc cttaatttcc
     3061 tctacatctg ttttcattat acccatttga acaagtatat ttttgtttat ttcttgttgt
     3121 tgtgtagata attctataaa attttctaca gtttttttat acatgtctct atcttctttt
     3181 ttctcctgca tagtattttt aaataataga gcacatagta tacctattgc ccctagacta
     3241 ctcaattcgg ttattaattt atccattata gcctcctatt gtctaaattt tttatactta
     3301 tagctttaat ttttgttgta ttcttacatc atgcaatatt atattttcag gattatcaat
     3361 ttaatcttct gtactttaaa ttgtaggtgt aatttataga ttgaataaat aataaatata
     3421 tatacaagtt ctaatgtaac aaaaaataag tacaaggctt acttattaaa gttaagacct
     3481 tgtactttac ataaaaaaaa taactaatac atagttattc taaatttact attcaactgg
     3541 tatttctaat tctttacatc taattctaac ttgctctcta attactgctg gtacttcttc
     3601 tattgttttt ttgcctctta aaattaaagc tacatatact tctaccattc cactctcacc
     3661 tccagatttt cttacactaa aagtgcttat attacttgct tcattgacag aactttgatt
     3721 taacaacatc tcatatattt ctacatttgc tataaaatta tttgttatat catcatcttg
     3781 ctttacttga cctatactta tgtctgaaat gtctttatac tgcatttcta cttttctacc
     3841 ttgtttgatt tgttctattt tatttttatc gaatttaaac aattttcaca cctccatttt
     3901 aagcttgtgg ctcgttgttt tcagcatcat atctaaaaat catagatgga cgttttatag
     3961 ctgtccattt atctcctttt tctactatga ttttaggctg agcacttttt atgttggctt
     4021 cttctaattc atagatttct tggtaacttg agtctgattt tttgatttca actagtttca
     4081 tgtctttgaa taagtcagat ttatttattt ttacttgaac tttagtttta tttattaatt
     4141 gatatatagg attgttttcc atcatatcat cctttaatcc ctcatttaat gttactatta
     4201 agtcagtgtt attttcagta atgtttatga ttgaatatac tttattgttt atcattaaaa
     4261 tagaatcctt ttttacttta agccctgttt ctgatttaaa tataaacata cttgaatata
     4321 aatcgcctgt aggactactg gctttaatca gtatcttatt ttctcctaat actattttat
     4381 tttgtggtat ctcaaatata atgctattta tttccaaatt agtcttagta gatattaatt
     4441 catcatttat aaacatttca agtttttcta taacattatt agggtcttgt actacaactc
     4501 tcacttgaga tattgatttt tcaactattt ctatagatgc tttacttgta tctacttttg
     4561 ttatcatgta tattgtatca tctgaaagct ctatatctaa aatatcattt agatgttcag
     4621 gaattggtat tttagtaaat tcatataaat cagtaattga tatagatatt ttatacatag
     4681 atttattttt accagttatc catagactat tatcattttt aattactagt atgtggtcat
     4741 cattatgtga cttaatttct ttaacattag ttaatacttt agtaaaagtt cttattatct
     4801 tatctttttc actttctcct agtccaaata atccatttgt attgtaccct tgagcgtaca
     4861 actctccctc attagttaaa acaagtgtca ttgagttata tatacttata tctttaacag
     4921 aaggtaatcc agtaaccttt tgtaactctc ttacatcatt attgtggcca actcccaatt
     4981 ggtactcagt attctttcca tatgaccata gagtattatc tgtcttaata atataggagt
     5041 agttatctcc acaagcaact tttttgacat ctgatatatt tacttcagta agagttaata
     5101 attctatatc ttcaagggtt tgattaattt ctttatatga attacttcct attccatata
     5161 acttgccatc cttagcaaca gccaatgaat gtgtttttga tatatcaata tcagatacct
     5221 caaatggcaa tattattgaa gtatacttaa aattatcttt aaatatgttt ttataattta
     5281 tatacttatt atttgttttt atatacttat cattaaacat tactagcttt tcatttttct
     5341 tattaaaaac tattttatct gatgtgttat ctgttttaaa atatgaatgt agggaacctt
     5401 caagagcttc atttttatca ttatcatatc tatggtgaat acctaaatga ttatatctat
     5461 tatctcctgt tgaagaataa aattttccat atatatcttt aataaatata gtattgtaac
     5521 tagaaaatac agtatcaaca tctttcttta tagatacttt ataatatgga actgacatat
     5581 ccctataaga ttcatcagat ataccataag tatctctacc tgatgatatc aaattgttat
     5641 tattatctat ataaagtgta ttattagctg ttacaaaaac atctttaaca tcttccacac
     5701 tatttgtatt attattattt gctatacttc catatccact aagacctcca acaaatagtt
     5761 tattgtcatt tgttaaaatg gctgaagtat atccattaac tactattttt tttgcattta
     5821 taatattatt aattactcgt atccccttta tatcattagt ctctgttgaa aagaataccc
     5881 tctctttttt ccctgtagta taaatcatat tgtcatttgt tataaaaaat gtagcttcac
     5941 ttcctgctgt cacaacagat tttacattct caatatttat ttttgtaaat gtacttatac
     6001 ttccttcagt aaacccaaaa tagccattta caagtccaca tacatagcat gtattatcat
     6061 ttttaacaat gattgataca tcattacata gatatacatc tctaacacca tttataggta
     6121 tacgtgtaaa tatattccta cttgtagtat cacccaatcc taactgtcct tttccattat
     6181 tacctgctcc atataaaaga ccatcattag ttaataatag agatgtgttt ccatatacag
     6241 ctacatattt tacattttca acatttattt tagtaaatat atttctattt acattatctc
     6301 ctattcctaa ctgaccagaa ttatttaaac caacagcata acaggtatta tcatttttta
     6361 tcgcaaatgc atgtttacta ttgtcagtaa atctatttac atttatagat tttatattat
     6421 caatatttat tttagtaaat acctttctat ttatctcatc tcccactcct aattgaccta
     6481 catcatttaa accaacactg taaagcatat tattattagt taataaatat gtagactttg
     6541 aatggctata tatttcttgt atattagaag atatttcact actgctattc tttttattgt
     6601 aatttatctt attatttaaa tttaaactac tattaactga tataccatgc gtacccttag
     6661 agttatatcc acatataaat aagctcttat cagttaatat tgcaaatcta gttttatctc
     6721 tacttaaatc ataaggaggt ataaatatat tttttatatt tttcattacc tttttataat
     6781 tagtggcatt atatccatct tgtgtatctg aaacatctag ttcttttgca ctttcagata
     6841 tattcccaaa accatatagc gtgttagaag ataaatacaa tagagcttta tataaaaata
     6901 tggctttatc agaggtaata ggacctgaat ttatattaga tgctgcaata ttattagatg
     6961 attgattgtt aagataattc cttccatatt gatgtatata agatgaacct ccagaaataa
     7021 gtaaattatc tggattgaca ttttttaaat tacttgacga ttccccatta tataaataag
     7081 caaaagttgt agaagatgat gattgtgtac atcttgtagg ataataatta tatatataag
     7141 aatcaagata tcctgcatat gaaattccct gatattgaac tctatagctt gaatcagttg
     7201 gttttgtcca tgaagtacca tggtatccag ttagaaaaga tccaatgttt gtacaaaata
     7261 cagttacata ctcatatatg ataatattat taattttagt aatattattc gtgcatgaaa
     7321 ctctactata acttgaccct gatatattta tataatacca atcacttaat aaacctagat
     7381 ttacattaga tccatcagaa gtactaaaac ctaacaggta atttgtattt ataccagttg
     7441 catatacctt tttttcatca actacaataa atgttgcatt atatgaacta taagcatcag
     7501 ttacattact aaggctaatc tcagtatatt gacttattaa tgcattacta agtccattaa
     7561 cataacttga actctttcca gaagtgtata tttgcttatt attagttaca attaatgaaa
     7621 accagtttga taaatcagta tttataacaa ctttttttac attatctata gatattttct
     7681 taaatacatc tgaattagaa ggaagaacac ctgctattga gttactattt ggtccagaat
     7741 aataacaagt atcatcattt ttaatgtaga atattcctcc agaaatattt tttgatgcta
     7801 ctatagactt tacattagag atacttaatt ttgtcaactc atttaattga cttggtaagt
     7861 cagaacccat tcctttgtat ctattatttc ctagaccata tacatctcca ttccctaaaa
     7921 taaccagagt acaataagga ctaaaaacga catctcttac agaatgtata tctagtggaa
     7981 gttcaatatc ctttatttcc atttcttctt ttaataaagt taatttatcc tcagatttaa
     8041 ttaaatttat ataattacta taactatatt tttcactatc cattttctta aattctacaa
     8101 tgtgcatatc atcaaaaaac gaacctaatt gtccataagt attatctcct gtacaatatg
     8161 aatcattatt tgaatcaatt agtatgctat gtgataaacc aatgaaaata ttcttagcta
     8221 caatattttt taaatctata aatgaattat ctatattctc agagtaattt gctataccta
     8281 actgatatat atcattattt ccagcaacct ttaaagtacc tttgttatct ataagcatag
     8341 tgttaccatg accacatgca atagttttta tatttacttc ttctatctta gtaaatagtg
     8401 atgattgaag tgcatttcca aaacattttt cctttccaca accatatata tcattattat
     8461 acgtcattaa cacagtgtgt tcagaaccac aagctatagt cttaacatca ttaatagaaa
     8521 ctaaactaaa taaatctcta ttattcttat ctcctaaacc tagttgacct tgattattaa
     8581 aaccagtaga atagcattta ttgtcattct ttattataaa agctgattta ttatatgaaa
     8641 atacagcctt tacattgtca acatctactt ttgtaaatgt atttacattt gcagatgctc
     8701 cattagttcc tagacctaat tgaccataca aattatttcc agcaacatat aaagttccat
     8761 cattgtataa aagatatgtt gattgaagtc cacatgcaat atcctttacg ccttttattt
     8821 ctggaacctt ctcaaatgta gaaactgttt tccctacttt ttctaatccc aatcccattt
     8881 gaccatctgt atttatacct gcgacaagta attctccatc tgaagtaagt ataactgcat
     8941 gagattctcc acatgcaact tttttaatat tggtagatat atttggtatt ttcgtaaata
     9001 attttcttgt tgtattgtct tctcccaatc caagttgaaa gttactatta tctccacatc
     9061 catacaagat attattattt tttagaccaa atgtatagtt acaacatcca gtaaaaaaat
     9121 taataaagtc ataattatca tacccttcat caaattcatc aactaccata tttttatcgc
     9181 caaagaaatt acctctttga agtagtttag ttcttttcat tataaaaacc tcctaattat
     9241 cttatttcat acgaatattt atttaaagtt atatctgtct gaccaattga atatgcccta
     9301 ttagattttg tttcaacaaa gttattatcc atttcatgta tatatctaac tctatttgca
     9361 ttaaatggta gcaccacatt cttaaatgta acactaccaa gttctccagt tatacacttt
     9421 ctatttaaat ctaacttaag gccttctata gctattatgt ttttagcatt agttaaggta
     9481 tcataccaat atccagcttc atctacatct atttctgact ctaattctaa gtatattaag
     9541 atatctaata ctttttcaga taatttattg taactatttt ttaatacaga tagttcagtt
     9601 tgtatttgac ctcctattac atcatcaata gatatatttt taattttttc tactacctca
     9661 tttatagctc ctgataagtt tttagcttct gtagataaga gttcttcgcc aatttttact
     9721 tttatttctt ttatattttt atcaaactta tcatctaaat cttgaatatc cttttgagtt
     9781 acaaaaacca acgtcgggtc tacttctaat gtaacatttg aagtattgga caattgtaaa
     9841 atcattttta caaccaaatc tttaatactg ccatcttcag cacgtggctt atacgtctct
     9901 ggatacttac ctatagctat tatattattc tcattatcca gaacagcagc ttctcttatc
     9961 ataaatccac caactgttcc tggtaaaata gtctctataa ctatccaatt aggatttttt
    10021 tcatcaattc ttagagaatt tatatttcct tcccaaacta cattctttaa agcagtttgt
    10081 tcctctgttg gctcataata ttctccatta ctatctccaa cttgaaactt ggttaatcca
    10141 acaagctctc ctagtgcagt tgcatttgct attttttctc taccaatttt ggttaatatt
    10201 atataaaatt tttcttccaa ttataccacc tccgatttag gataaactac tacattttct
    10261 acacctgtat catttgcaat agctatatta atcttacaat tattttcaat gcatttaatg
    10321 cttaaaggat aaacctttat ttcttctcta cttatcattc cagctccaca gtttacaaca
    10381 gcactacatt ttataattgg ttcaaagtat ggatgaactt ttacctcttc actacatacc
    10441 atacctccac cacaatagac catactttta tctagtatta ttggttctaa atagtgtaat
    10501 aagtgagcag gttttactct ttcaatcata tcgctacaat ctaaaagagt tttatagtca
    10561 cagttatttg ctataaaact caatacgaat gtaaattcat cactataaac ttttatatct
    10621 gtttctgatt ttgtatatgc ctcacatata ctttttataa cttcaatagt actagtacct
    10681 ctgcttctca ttttagcttt tatattgcta cgtcttgtgt caaaatcaag ttcttttttt
    10741 tcaatgcaga gtatgtcttc ccacatatct aatccccatg ttgcagtgtc aacaaataat
    10801 tggttaaagg tatcatcaat atcttcttta agaatattta attctgtatc gtatgcacct
    10861 tgtatctcct ctacaatgta atttctatca aatgatggta gtttatcaat tagcttcatt
    10921 gattttccac ctcactaaaa ttaacagttg ttacactagg tattttatct tcattaatag
    10981 ttatattatc tgtacttcca tttataagta ggttacttaa atcgtgtaca ccagtagtat
    11041 ttataagtat tcccattact tttgtataga ttatctctcc tctaatatct ctaaagtatg
    11101 tatttatact ttctaggaaa gattctttta cattgtctaa tgtatatcca tcttctagtt
    11161 tcattactgc acttatactt atttctattg gtaatggtgt cacaactgtt atagttggtc
    11221 caataggctt ctcttcatct atatgttgct gacacctttc aattgtttct gtatcaacag
    11281 cttgattatt tttcccaaat atcaagacct taactgttcc cggaccatcc catcttggat
    11341 aaaccttaac attatagact ccatctactt ccaaagccca ttcttcatag tgagctttat
    11401 ttccacttgt agcttgattt ctttggattt tataaaatct ttcttttagt tcttcatctg
    11461 tctctatatc tgtaccacct tcaaaacttc ttgtgttagt aatctttgtt actccagata
    11521 tattatcaac tagtttaaat tcacaatttg taggtaaatt atatttctta ccaacttcca
    11581 gagcttgaac tgggctattg tcaccttctt cactaccaat agttacatct tttattacta
    11641 caaatagtaa atctctatat gatattattg tgccattatt tattacagta cctttctctc
    11701 caataaattc aacttctcca tttgactctg tacctaactt tctatataca ccaaattcat
    11761 tgactctttt atcaagaaac tggttatatg tgtcttgtat aaaagccatt ttatgcatct
    11821 ttgaaagttc tagatatatc ttcgaaagtt ctagattatt tccagatacc atgttgttta
    11881 gaaaagaacc ttctccttta taaatatcaa gattaatatt ttcaagagtt ctatttttta
    11941 ttacttcata tgtctggtca ctatacattt atttccacct ccccatagat agtggatact
    12001 tttacatttg cactcaatat gtctctgtta aagttagcac tttttacgtt tacttctaat
    12061 atatatggat ttattagaag ggcctctttt atgaatctac tagcttcact ttctgtaaga
    12121 ccttttgtat atttttgtcc tattagttct gaaagctctg tcccatattc taaagagtat
    12181 atctcatgtt catatctatt tgtctttata cacttgtata tccaaacttt taaggcttct
    12241 tttttttcaa ttattttaaa gtcacctttt tctaaaatag gttcatcttt ttcaaaatcc
    12301 catgccactt cacgaaagat tggcaattct tctgttttag gtaagatata atcctctggg
    12361 acacctataa aaggaaatat tgtactcatt ataagctcac caacttactt acaacagcaa
    12421 atttttcgcc tattctaaac attattacca tatccccaga ttcaaagtta tctataaaag
    12481 gattttttac ttcatgctgg tgttcttgat ttgtttctgt gtcaaatgtt tctagctgtc
    12541 tatcaatcat ccaactatct atcaaaatat cttctttttc taatatgatg ttatttgtct
    12601 ctatttttaa atctggtaat ttacttttaa tttttccaat aaaaaaagaa ggttgattat
    12661 gatactttcc ttcttctctt attattccta taaattcatt tattggatta gccattatat
    12721 caccatcctt ttataatact ctcctagctg tgttaaaatc tcttctacta cttaacttac
    12781 ttattttaac tacatcacct ttttgtggag catgtaaaaa ttctccatta cctatatata
    12841 atccaacatg gctgactgga ttatgaaaaa atactaaatc tcctgcttga agattttttt
    12901 gttctacttt cttgcctttt ttagattgtt gatttgacgt tcttggcaaa ctaacattaa
    12961 cttttttaaa acaatagacc attaaaccag aacagtcaaa actacttggt ccatttccac
    13021 cccatttata aggtttacct agatgttttt ttgcttctga tattactatt ttagctttat
    13081 cagatacatt accagaataa ccaccaataa taatttttcc ttttcttctt ccaaatttac
    13141 tagcttcttc aacatttcca aataaaatat ctatatggta tgttccattc ttttctatag
    13201 ttattgcagg tcctacgtca tttactttat agactccatc acgaccagaa actcctgtaa
    13261 ttacctgtat ttcatctcca tagtttaata aaggatgttt atttaaaaac tcttttgtat
    13321 aataagtttg ctcatattta ccaaccatag gagcagcgca agttttttta gatgggtcaa
    13381 gttttttctt tctacaatct gtatctccac cttcttcttt tctaggacag taagctgtaa
    13441 attctgctgt aaactctttt cctcctgcat aatcttctcc ccctaaatta ctttcttcct
    13501 taggttcatc ctgtcctgct gacttttcat ccataagatt ttgaaaatta agttcaagct
    13561 caatttgata ttctccattt tgccaagtat gtttgtctgt atctatataa aatagtccta
    13621 caagctttgt ataagaatct ttaactttta ctcctctacc agttatacaa cttacatctc
    13681 cataaccttt aagagaacag cttttttcta tcccattaaa ctcgctatca atatctacat
    13741 cttgattttc ttgttgctga attacttttt gcattattac atttacttcc ttaaaaattt
    13801 cattatctat tttttcgcta atcttgcttc catactggtc taccactatt actttatttt
    13861 ttacattttc catgctctcc gaaaaggtgg tatttataat attaaatccc tcttcaaaca
    13921 taacacttaa tgtaacagtt cctttttcaa taacattaaa cttatctaaa ttggcctcta
    13981 tcatatactt tttctttgtc tttttacttg cttctgtata tgcactcatt attgtgtcat
    14041 aaccatttac tcctataaac atctttgtat acttgacatt ggtctttgct attattccaa
    14101 ctgaaagcct attttcagca aatacttgct ttgctatgtc ttcaactaac ttatctttaa
    14161 aattgtatga cacttcactt tgtgtaagta aaaatcccat atctttagat acaaaactaa
    14221 tttcattact gcttgaatct ttagacctat taattatcat tcctctaaag agttctttct
    14281 catctacata gaaacagact gtactagcta ttggtatatc gatttgttgg aaatttacat
    14341 caaatgatga ttgtattatt gaaaactcta gtgtccttga tggagattta taatcacctg
    14401 accatgatac cttgtctact atgtcagtta tgtcatatat acttccgttt tttatgtgta
    14461 cccaaatctt aataataaaa aatcacctgc cttttcattt aattctatca gcagttattt
    14521 ataacttatt atctataata gttattgttt tggagttact tttggtatct ttattctttt
    14581 atactctttt aaatctaatg taaaatatac atctcgagtc ccatctcttt cttcataact
    14641 aaacccttca attatgactt ccatatttat atttgtttcc gtaattgtaa atcttaatat
    14701 aaaaccttcc ttcatccatt tttctatctt atttacacaa tcatatggtg atggaaaacc
    14761 tgtatagtca cagaaagtat agtctttatt tggaaaaaaa cttgatattg aaatgctttt
    14821 aagagctaca ccaccaaaag ttgctatttc acctattttg agtatactag aagagtttac
    14881 agcagctttt ccattaatac taaaggaaga tggaaatact ggaaatctaa atctatcttc
    14941 agcttgtcta agccacattt ccattataag acacccccta actgtgccaa ttggatactt
    15001 gcaactaatt ttgatgttat cttttctata tcagcttctt ctctaatcac tattgtatca
    15061 gctaacttag ctatgtttat atttccacca ttttttctgc ttctatattg atttgcttct
    15121 tgtttagtta aaaccatttc accttcatgg agtctggttt gatagtcatt ataaggaaca
    15181 tagttcaatc caaatgcatt attacctttc ttacctgaat aagctttacc agaatcacca
    15241 gcctttgttc cagcatatgg attatctttg taattttttt tactcttttc cactagattt
    15301 aaaactgttc ctgtgatgct tccatttttt atcttattaa tcatagtttg ccaccattta
    15361 catatatctt taactatttt acatacttta tctaatatgt taaaaaaagc tcccaatatt
    15421 ggctctataa ttttccatcc agtttccaac agaggtccta aggtctccca tacagttttc
    15481 caaataacac ccagtgtctc tataaaattg tttatttgag gtgcatattc acctaaaaaa
    15541 gtaaaaattc ttgtgataat aggactaaca gcttcaacta caggtccgat tagttggaat
    15601 gctgtagtta ctattgtacc aaatatactt attaatcctt gtgcaattgg tgatttattg
    15661 tttatactat ctagtaaatt atttactaag tttactactg gaagtaatga atctattaat
    15721 gctgtaaaat cgatattttg taatagtgta cttcctgcgt taacaattgc ttttggtgca
    15781 ttccctatca ttgttgctgc atgattttca aaacgttctt gcttattttc aaccatcatt
    15841 ttacgctttt cagcctcatt agaaattcga tattgagtag aatcagaaaa ctcttttcca
    15901 ttttttacac ttctagattg catctggtca tatttgttgc caataattat cttaccattc
    15961 attacattgc ctattttacc agccattttt tcagataagc cagctaaatt gcctaatcca
    16021 ctttctataa aagtttttat tttctttagt ccttgaccta aaatatcatc aaaaggttta
    16081 acaatactct tcaagccatt gttaatgctt gtttgagctc ctgatattat acctggtatt
    16141 gtattactca tttctttagc taaccccatc tgctttacac ttatgccttc caatgcttca
    16201 tcaaaagttt tataattttc tcctaccaga gtattaagag attccatgtt tccttttgct
    16261 gcacttaaga aagctgctga tacatcttgt tcactacttg tattcatatt taaagctcta
    16321 acatctacca tagcttgagt tatatctttt tcttttttgc caccagtaat attactaatc
    16381 attttagctt tagttcccat tgcaacaaca tcttcagggt caaaaggcgt atcatttgcc
    16441 attcttacta aactgccata ataatcatca acatctattt gattaacttt tccagtattt
    16501 cttttcatta cactttctag aacaactttc tgattttgtt cctgaccaag taaatcaaca
    16561 ccctttttca cagcgacacc tatagtagca ccatttgtca aatcagatat cttccctttt
    16621 atcgaaccaa gtattccact tgccatatcc tttacagcta ttgcaccact ccatacctta
    16681 cctgcaaata atctcaattt gggaaatact ttacttataa tgccacttgc catatctttt
    16741 atagctatta caccactcca aaaagtattt ttaaacaaca ttaacttagg caaaattcta
    16801 cccatagcac tactcaccat atctttaaga ttaatacttc cttgataagg acgtttctct
    16861 acttgtttta attttcccaa gatactactt atcactctct tagtcatatc ttttgctttt
    16921 attacgatat tccaaatctt gccaccaatc ttttttaaat ttccaagtaa tttattttta
    16981 gttttttcta cttcatcact tttagcttta aatattaata taatcataat atttgaatta
    17041 aactcatttt tcttttgaat caacttatta aaataatcaa taatcatagc tatagttatt
    17101 gctagtctca taatacttcc atttaaactt ccaataactc ctgaaacagc atttatgcta
    17161 gaacttaatt ttgtcatagt tgaacttaca ctactagcag tagtttgaac ttttcttaac
    17221 gaggaattta aactgtttac tctattactt gcacttccaa tagaattttt tatgctacta
    17281 gccattacat tagtggaaag tgaaacagcc acagttgctc tcctaaaatt atcagttact
    17341 gatataattt gattcatttt tggtgtgtaa ttatctgtaa ggtacatatt tataccttct
    17401 tctctcacat ttcccattac ataccacctc ctgtcaaagg acacactcca tatccatgtt
    17461 tcattttttc aacttcttcc tcaatttcta aggcgaaaaa agcttcaata agctttaatt
    17521 cgcctttatc cattgcataa aaaagggacg gtcttatacc ttttttcttc caataatagt
    17581 acattattgt agtcctggta tccgtcccta ttagtttttt acctcttcta tcactgcatt
    17641 tttaccataa cccataacat cttgaagtat tctatataag ttggtaattt cacctggtaa
    17701 taataatttt cttgctaatt cttttggtgt tggagcctta aatttatcca ttagctcctt
    17761 atttttaaat aaaagctttc catccaaatc acaaacacct tctataattg taagcatctg
    17821 catctccggt aagtctatat ccgttttcat attttcatca attttaagac aagtattttg
    17881 tatttcttca tctttactta ttgttaaagc tctacacatt actttaaatt cctgcccaaa
    17941 tatagttgat aatctcttaa gttcaactat agtacttggt ctttctaagt tttctgtatc
    18001 tgaatttaaa agtaaatcta ctatgtttat attttttgat aatccatttt cactcattat
    18061 tatttgctcc tttatcaatc aaattcaatc aactaacttt tttacttatt aactatctga
    18121 atttattaag tatatactta ctaaatatca gtataaaact aaatataaaa ctaaattata
    18181 tcaagaaaat catactcagt aaatgtgaaa ggagcttcta tctctccttt tactccaacc
    18241 tcccaatcag ctaaagttaa atcatcaaat gctatatttt ttactgctat tctttctgct
    18301 ccataagaat ctgggtcatt taattttgat atagcaacaa atctaggttc agaacctctc
    18361 tttatctttt ctccaattaa cttgtgcatt cttgaactaa cgtggtatag agttattgag
    18421 ccttttcctt tatatcccat atactttgta tcagtaccca tttgacctgc tattataata
    18481 tcttcttttg taaattccat ctttgcttga aatttcttta cttctgctac tttgtttcca
    18541 tcaagccaaa gttctcccca agtaccactc attacatttc tagcttccat atttgccata
    18601 ttaataatcc tcctatattt ctattgataa atctatatct tccatagcat caagtacttt
    18661 tatttttgct tttaaaaata ctttagaacc tgtgttagct tcttttattt cttgtaatgt
    18721 catataagat aaatctactc cttttgattt taaatacgat ttttgtgctt caaaatctat
    18781 ttcaacagta gaatcagatt ctataagtgc tgatttttct aattcttcta aataactttt
    18841 tatagctact atcaataaac atttgttgtc ataactgtta gtaacctttc ctatatagtc
    18901 atctattatc acctttctta tgtcactatg tataatatct aaagtgtcaa ctatttttat
    18961 tttctggaac atttctcctt tttctgctgt taattcagtt aaagaattta ctcctctagc
    19021 aattcttata gctcctgctt ctttaataag tataagctct cctttattaa cccttgattc
    19081 tgcatcaact ttcgtcatct taggtatatc gactacatca ctaagtttag tataagttac
    19141 tgattgactt aaaggtgtac ctgctataag tccagccacc ctgcttgtaa actcatcaac
    19201 actgtatttc ttttctccaa ctaacacatc ttctgtagta aaattaatta tcccttcatg
    19261 atttcctaca acttttccta atacagcttt aaccttaacc ttatctatat ctctaagttt
    19321 aattatccaa ttttttatag cagtcttatc agcttctact gcttttggca tacatagata
    19381 attaaattcc ttagtctcta aaaaatctaa tgcagtttga atatctgctt ctccttctat
    19441 tacataaact aataatttat ttggagtgtt aacatttccc atcaaagcta aatttatata
    19501 ttctttattt tcagcagata aatcaactgg tatatcctct ttttcatgta tttcattaag
    19561 acctagtgcc ttagcatctt taagtaccat tgcaattatt cctctagctg aacgttcttt
    19621 aacagttgta gctagctcct taaatgatat gttgatactt ggtaatccta tagccatact
    19681 atcactctcc taaatttaaa ttgatttctt tcatattttc atatactaca tcttcttcat
    19741 atacactatc cagatagttt agtgtaaatg taaagttcaa tacataaatt gactcttctt
    19801 ttttaatact ttgttctagc tttgttatat ttaattttct gtctgttacc tttatatttc
    19861 tgttaaatag cttatttaat tcatctgaaa tttcatacat tctaatactc tttgactgtg
    19921 gttttggtaa gtattttata gagataacaa tatctttgtt attcgtattt aacattacag
    19981 gtgtacaaat aactgatagt atacttacaa aaaaacaaga cccttcagag ccttgcacac
    20041 tttcatcatc aataaaaata ctttcagtaa agtttttttt gagaatctca ataattgttt
    20101 ctaatatttc cttgtattta agcaaataat caccacacta tcactaaata ttcttgctta
    20161 atctgataat cttactataa atgatataag tcatttttaa atatatatct catttattcc
    20221 tgtcaagtat cttctatatt tttaggtaat attattcttt tcaatctatc acctccttca
    20281 atatggattt ctttcacaat aacattatct cacatatttt attgcctatt gttgcaatgg
    20341 tgtatataaa gtgttgtaaa attgtcctat tttttataat atctcactca atttaaatat
    20401 catctttttc tttaaattgt agcaagatgt tctatctaag tgtagtttca tagaaatata
    20461 tgtcatattg ttttttgtct tactattata aaaaagatta aaaaattcca tttctatatc
    20521 atttagacac gttagagcat tttctatctt ctctttttct atttctatat cttctttttc
    20581 catttttaat tttcttaatc tttcctctct ttttaacacc tcattttcca cagaagagct
    20641 tatgttatat gtgttactag tcttttctgt atataccatt gctccacatc cagagtattc
    20701 attctcacat ctttttatgt ccaagttaat attctttatc tttatatcca aatacttata
    20761 attatgtaat cttttatctg tttctttaaa taatatatct tttttgtttt ccatagtact
    20821 tccccttttt tatatactta ttattagctt caaatttaca ataatctctt tagtgcacag
    20881 ttttttacta atctcacaac tttcaagaac atattgttct ataactgtat aaaaaaaact
    20941 tcttctatat tttgttctaa aatactagaa atcttcttag ccataagaag tccaggattt
    21001 tttatacctc tttctatttg agatatataa tcatttgtaa ttccagcctt tctggcaagt
    21061 tgaatttgtg tcataccttt gcattttcta taatatttca agttattttt aaacattgtt
    21121 ttatacctcc attactatta agaatatttt gttcttcctc acttagatta tatagaatta
    21181 tttgttcttt atcaatagat tttagaaaaa aatattcttt tatattttat tagttttatt
    21241 ttttagaaca atttgttata atctatacat aaggtggtgt tataatgttt gccaaaagat
    21301 taagagaact tagaaaggaa tttggattga ctcaaagaga acttggagaa aaagtaggcg
    21361 tttcccaaag agtcttaggg tactatgaaa cagaaaacag atttcctgat gaacacattt
    21421 taaataaatt ggctgatgta tttaatgtat cagttgatta ccttcttgga agaaccttag
    21481 ttaaggaaaa tattgataca gtagctgcac atagaaaaaa tccacatgaa gagttacctg
    21541 aagaagcaca agaacaactt aatgattata tagaattttt actaaataaa tataaaaaaa
    21601 aataaaccat atgatgagca gtctattttt ctgctctttt aatataattt aaaggcaaac
    21661 atacattcta ttttaggggg atttttatga ataatttgga taagcttttt gaattagctt
    21721 ctcaagaaga gataataatt cattatacca cttatattgc aggtgattta gaaggactat
    21781 atataaacaa acatggtatt aaaattatat cattactcag taatttaaaa caaaactcaa
    21841 aaaaattgac atccatctta gctgaagaac ttggacatca tttcactagc cttggatact
    21901 atgtatcctc ttataacgat tattatacaa aaattattat agataagtgc gaaaataaag
    21961 ccttaaaatg ggcttgtgag ttcttaatta ctgaagaaga tataataaac ataattaact
    22021 ctggcatcac ttgcgtttat gaaatggcag acatacttaa tgtggatatt actttctttc
    22081 aaaaaagatt agaatttcta tcattaaaaa aacagtcttt acaacttgga aataataaat
    22141 atttaatatt aactaatttg ccgtatttct acatatttga tccaatttca taataaaaat
    22201 agtagtaatt ttaactggct taacagtaaa atctatatat ttattttaac aaattttggt
    22261 ttctataaat tcaatattaa tatataataa tgatatcata ttatttaaaa ttctaaaaac
    22321 aaatatatgc ttttgttaag aaaggttgtt tgatgaatgt ataaagaaaa gttgtttgat
    22381 aattatttta agttcttagc actattattt tggccaatta tgtggtataa gtggatagta
    22441 atttcaaatg gtactcttga aaatatgtta tttaccatat atgcaatcgt cgcaattgtt
    22501 ttcatcattt tatattcggt gtttatgata aaatataaag acattacaca aatagatttc
    22561 ttttacagga taagtactct acttgctttt atatttacac tttttagctt tttaatatac
    22621 ccaaagagtt tatttttctt gtatttaaag attattttta ctggaatata cctttattat
    22681 tcaatagtaa aaactcttaa atttaaagat gacgaaggtg tagtgggtat tatgagctca
    22741 cttttattaa ttgtaataac tttgttctac taaatttaat tctatttgac ttaatatggc
    22801 tttaaatatt agacatctac attattttct gatattattt aagatggtag tcttatatta
    22861 ataatgaaat acactatgaa ggagttaact attatggaaa aaaaagtttt agcaacagtt
    22921 ggagaaaaag agataactaa tattgatatt gaaaatgcat taaaatcatt agacccttat
    22981 caagctatgc aatttaaaac tgaagaagga aaaaaacact tattaaatga tttagtgaat
    23041 caagaattat tcttcttaga tgctaaagaa gaaaaattag atgaagaaga aatatttaaa
    23101 ttagaaatga aaaagataga agaaaatgta ttaaagcaat ttgctataaa taaagtttta
    23161 tctagtgtta atgtaacaga agatgaaaaa gtaaaattct ttgaagctaa taagagctct
    23221 ttctctaaac cagaaagtgc aactgctaaa catatattag tagatagtga tgaaaaagct
    23281 aaagaaatac ttgctcaaat aaaatcagaa gaaatatctt ttgaagatgc agctcttaaa
    23341 cattcttctt gcccatctaa agatatgggt ggtgatttag gaacattcgg tagaggacaa
    23401 atggttcctg aatttgaaga agctgtgttc tctatggcta aaggtgaagt aagtgaacca
    23461 gttaaaactc aatttggata ccatataata aaattagaag accttcaaga aagtacagag
    23521 tctacatttg atgaagttaa ggctgaagtt gaaaaaagtt tattatatca aaaacaaaat
    23581 gaagtttatg gaaataagat aaatgctctt aatgctaagt acggaaacct tgtaaaatac
    23641 aacgattaat cataaggtca tatataaaaa agctgtcttt tcacaatatt tattagaaaa
    23701 gacagctttt tatattaaag tttaaatatt cagtttcata ttatcattct tttattgtaa
    23761 agaactcagc ttaaaagttt aaataagatt tttaattatc attagctaaa tttttatatc
    23821 cacgtattat ctcataacag taacctatta catctcttct ttttattatc ccaataaatg
    23881 tgtcttggtc atctataaca ggaatgaaat tttgatttaa agattttata actaaatctt
    23941 caatatctgc gttgatggaa acaggagaat tatccatttt tctatttata tctgtcactg
    24001 gaacatcttc caatcccttt aagtccagat ttaaatcatt ttttaatgtc cataaaaaat
    24061 caccttctgt tatagttcca acatatttac catccttact tattattgga attgcagagt
    24121 atttgtgata ttccattttt tctagggctt gccttatagt gtagtcttca tatatatatg
    24181 ctacttcact ttttggtgtt agaaagaata atatgttcat tacactaccc accgttactg
    24241 aaaacttata aatattactc aatatttata atgttacttt tttgcagtaa ctttctcctt
    24301 tcttattttc taagtattct taatactata cgctgtaaca agcgtttttc ctttgctcat
    24361 tttaatctta ttatgcttta ttaatatttt tatatggtct atcagtatca cttattctct
    24421 ttttagaaaa tttactaaaa attaattgac ttaaacttag ccataataat tttactctac
    24481 actattaatt ttaacatatt tatctcatta ttaatgattt tattttaata attttaattt
    24541 attttgctat taattttatt tacaaaaact gaaaaaaccg ctaaatgatt agcggcttat
    24601 ctctcatatt actagaacac taatatatat gaatttttaa ttataatcta actacgttag
    24661 cagcttgagg acctttagct ccatctacta cttcgaactc aacgctttgt ccttcttcta
    24721 atgttttgta tccatctcca gttatagctg agaaatgtac gaaaacatct ccttcacctt
    24781 ctacagatat gaatccaaat cctttttcgt tgttaaacca tttaactaca ccagttttca
    24841 ttaataaatc ctcctaaaaa aacacaaata tttagctact cttttctgta accttattac
    24901 tactctatca tatatagcta tgtttgtcaa ttattattat ttttatacat taataatcaa
    24961 tttttttact ttataatttt aaatgattat tatcttttac actttatttt tctttataat
    25021 tttttaataa tttattttta ttatatggaa tcttatggac ttatcactcc aacctaaatc
    25081 ccatggcacc aacattctat cagtattatg acaagttacc aaaggatacc ctcttgagtc
    25141 taatcctgtc actgtcgaaa catgtgttat tctgcctttc ttctcataac caacaaaatc
    25201 ccctggacgt aaattataag cttctttgta tacttcttca taggaacctt tagcaattaa
    25261 tgaacctctt ccactataca gtgcataatt tttaaaagct tgtgcattta cccatgcttt
    25321 tgtacctgct ctctcgctat aattccaaat tgagttcttt ttgaaccttc cactttcaaa
    25381 cattatctgt gatgcaaagt ttgcacaatc tccaccttct ggattataat ccctatagtt
    25441 tggattaaat ttcataccat gttcttcatc agctgcggct ccacagtatt tatgtgcata
    25501 atctatagcc tttttttgtt catctgtaag ctgtaactct acaccatctt gttgaccaat
    25561 ataatttctt atatcatcag attttatatt ttctaaattt aaagagtctg caaaagggtc
    25621 tgtataccac tctttagtta tgacatattg attatctata atttttacat ttagataatg
    25681 atatgtacct attctaaagg cattttcttt gtccttttga ttttcatatg aatacttata
    25741 ttcagtcgat actgaacaga ttataccata gaggtctttt tctttttctt ttattttttt
    25801 aatcttaacc cttgtcttta tgtctttaaa ttttacacct tgcttactag accaattttc
    25861 taaatacttc atctttttta cttcattttc ataagcccac agaccaaatt ttttatccgt
    25921 ctcataaaga ccttttaaaa tgttttcatt tttctctaat attgccttat ttctgtaatc
    25981 aaataagttt tcaagcaata gtttatattg gtcttttaca tcatcacctt caacatttgc
    26041 agattccatg actttttctt ccatattttt catacttaaa tcattgatta aaaatacacc
    26101 tgctgtagta gctaaagaca aaaatacaaa tgccattata cctttgatgt acttattctt
    26161 atttttcagt attttcaaag ctacacctcc caaatagtac accttacaaa tcaaattaca
    26221 aagaatattc tgtcacatat agtaattttc atatttaata attctctatt ttattatgtc
    26281 caatattaaa aattaattgt atcaatacac tgtttttttt acaatttttt attttataat
    26341 ctactttttt ctttttttta tagtaatata tatgtatata aatattttgg tatcattata
    26401 attaatacat actaatggag ggttttttat gcaaaaaaaa gtcgcagcaa ttaatgactt
    26461 atctggaata ggaaaatgct cattatcggt tgctatacct atactttcag cattaaaggt
    26521 tcaatgttgt ccatttccaa cggccatact ttcaagtcaa actggttatc ctgaatttac
    26581 ttttttagac tttaccgatg aaatggtaaa atattcaaat gtttggaaaa atttaaaagt
    26641 caattttgat agtatatata gtggtttttt gggttcaaag catcaaattg agatagtggc
    26701 taattttata aacgattatc ccaatgcttt tatcgtagtt gaccctgtga tgggtgataa
    26761 tggagttatg tatccaatat tcacagaaga aatgagacat gaaattaaag aattagttaa
    26821 acattcagat ttaactactc ctaatttaac agaagcttgt ttcttaactg gaaatgatta
    26881 taccaaaagc gattataata gagatgagct tatatatatt gcaaaatctg tatctgattt
    26941 gggacctagc aaagtcgtta ttactggtat attagaagat gataatatat taaatttggc
    27001 ttacgataga gataatgacc atgtattctt tacatcagtc aaatataata attgttctta
    27061 tagtggaact ggagatatat tcacatctat actttgtggt atgttagtaa ataaacatga
    27121 tttgggagta gcggttaata ctgcaacaga ttttatatat aaaactataa attacacatc
    27181 tcaatttgat actgatagaa atgatggagt tatgtttgaa aactttttat cagatttaac
    27241 taatatataa agtcaaaaaa actatccatt ttataaatac ctataaatca taaaatttag
    27301 gtatttacaa aatggatatt atttactaaa ctattggtat ttcaattgtt accatagcac
    27361 caccagtttt cacagaattt tcaatatata aaatcccatt atgtttttgt acaatattat
    27421 ttacaatata gagtcccatt ccataatgag atttagaatt tctactcaaa tctcctgtat
    27481 aaaactgttc tgtagctttt gacaaatctg ctttagaaaa accacttcca caatctgtaa
    27541 ctacaaattt taagtattta ttacgtactg atacacttat gtatagttta ctctggcttg
    27601 gtgaataatc tatagcattt gaaattatat tcataatagc tctgaataac agttctctat
    27661 ctatagttat atttttaggc aaattatctt gtttataaaa ctccacatct aagtttttag
    27721 tactagctaa tgcttctatt tgcactaaga catcttttat aaactcactc atactgattg
    27781 cttctttatt taatatatag ccattctctg tttttgatat atctattaaa gtattaatat
    27841 atttttctat ttgagtgaca ccctttgaga tatattccat gtattctaat aactcaggat
    27901 tttcatttat ttcaattaat aagtcagtat ttccatgtat aattgttagt ggtgttttta
    27961 tatcatgtgc cagtgcagat atctgttcct ttctattttc ttctagcatc cattgttttt
    28021 ctaaagaatc ttttaacgcc aacttcattt tatccataga aaaaaggaca ttattaattt
    28081 cataaatttt actagattct acaacaaaat ctaaatcctg ttgttctatt ttttctgtag
    28141 tattttttaa aagctccatt tcaatattaa atttcttgcc aaatactttt gataatataa
    28201 caatttcaat aaaaaatatt atactaaaaa taatcatacc caatgtctct ggttttggta
    28261 aatatgctct taaaattgga gaactatact ctgatactat tttgtatttt attataaaaa
    28321 cttcatcttt cttaaaaaac tttatataac tttctgaata tgcatgcctt tcttcaatat
    28381 ctctcattaa attccatact tcttttgatt cttttttatt aaaggtccct gaaattaaac
    28441 tcccatccaa agtataaacc ccatactcat atatacttgg aattatatct tcagttactt
    28501 tttcacttga aataatctgt tctttatatt tattaaattg tttttctgca taattagctg
    28561 gtaaaattac acctgatgat aataatacag aaaatgataa cattaaaagg agtacaattg
    28621 aaattgtcat aataaagaat aaagataaat attttataaa aatagttctt aacttttcaa
    28681 cttttttgtt gttagatttt ttacctccaa gcatttgtac cctgtcctcc aatagtttaa
    28741 ttaattcata attaatatct aaccagtttt ttatttgatt attttatatc tttttattgc
    28801 taagttatat tttaacataa taatataaat aaacagtcaa tttttactct attcttctga
    28861 ttttttacct tcccatttag aaaaccaaat acataaaatt acaaatgcta aaattgttac
    28921 aaaaccacac attaaacctg ttgattgata tgcttttatt gattcaacct ttatatattc
    28981 aaccgtttta gaactcgaaa aagacgacca aatagttata aatctaacac cccatgcaca
    29041 aggaaaatac tgccaaatac cagaacccaa tccagttaat aataatgctg aaagtaacat
    29101 ttcaactata cctactccaa ttgatgttcc ttttccgaac cttaaactta agaataaatg
    29161 taatatgtat tcaaataaat tacacccaat taaaattaga attgaaatca tataaaagct
    29221 tgatgcaaag taagccttag atataccaaa tttaaaacct aatacagaca ctaatgttgc
    29281 tacaaaacca agacatacta agattatata tttacttata agagtaagat attttggatt
    29341 agatgttgtt aacatatgtt tataattccc tgcataatat tcttgttcaa caaacattga
    29401 acacacaaca cctgacaata aaggaaaagc tattgctata acttgtaaat atgctactat
    29461 ctttagtata ttatcagctc ctgaaacctt ataataccat aaaaacaatc ccatccccat
    29521 taaagcaatg caaatgtgca ttagtattat aaaatttctc tttaatttta ttaaatcaga
    29581 acaaaatatt cttattaact gtctcatatt acttcacctc tctattattg aaccaaaccc
    29641 ttgtagctaa agcaaataat acaaataaaa caatagatat taatactcct ggcaaaataa
    29701 cagcattatt taaaaattca cttccctctg gtacaggaag accatttggc agaatatgca
    29761 atacaacagc cattaatctt gaaggaatcg aatatggaaa catataccac atttctgtag
    29821 ttgagaaaac agcaactcca acaatagtaa agaacatatt tattagaact gtaacaaata
    29881 taccaaattt tgaagctaaa aacatacaaa gaggaatctg ccacataaat agtaaaataa
    29941 ttaatatagt tgcaatgata ttttgaccta ttgaaatagt ttctttatag attatcccaa
    30001 caaaaactac acctattgca gaaataattg atgctataaa acaacaaata atacagtaaa
    30061 tcatttttcc agtccaaacg tctttcattt caattggcaa tggtaaaata ccacgatatt
    30121 tacttttttc atcttttaat actattaaag aagatattaa tgttaatgct cctggaagaa
    30181 atgtcgtata ccaccagttg aaagcagcat tttgccctcc acctagtcca cataaaagta
    30241 tacaaaacac tggaactatc catatcaact tcattccaaa agttcctttc atcttttgaa
    30301 attccactct aataaatctt tccatattac tgccctctct ttctattttc agctacaact
    30361 ttcataaaaa gtttttctaa gttttgttct tttgatactt ctccttgata acccattact
    30421 ccattagaaa ttattcctat ttcatctaca acttgttcaa cttcagacaa gatatggctt
    30481 gataaaataa ctgtaattcc ttgttttgga aaagaacgaa tcaattctct aagttcttca
    30541 ataccaatag ggtcaagacc atttgtaggc tcatctaaga ttaaaagttt tgggtgattt
    30601 aataatgcaa tagcaatacc aaggcgttgc ttcatgccta gagagaattg acctgaacgc
    30661 tttttaaagg tatctcttaa atctacagtc tctaaaactt cttcaatgcg agagtcagga
    30721 agaccaagta aagtagtatg gacttttaaa ttttctcgtg ctgttaaatt ctcatatata
    30781 gcaggagact caataagaga accaatattt gataaatcct ttctagtcca tctatgtccc
    30841 tcaaacatta tttttcctga agttggattt aaaatcccag ttatcatttt taataaagta
    30901 gatttcccag ctccatttgg tcctaataat ccatatatag aattagattt aattgctagt
    30961 gaaatgttat caactgccat ctgctcctta aattttttag aaactccttt tacctgcaaa
    31021 atataattac tcataatttt catcctttct tttgtcaaac ttatttacaa gaatcattct
    31081 aaatcataaa tataaggatt ttataagaat taaaaaacaa aaaaagatgt gttaaaaaca
    31141 catctttttt tatcttaatt ctattaattt tccattgtag tatatctcta cttcttcaat
    31201 ccaaatgcta ttatcttctt cttggatgac atttttaata atattattta tagtatatat
    31261 tatattttgt tcattcaaat aatcatcttt ccaagaaact ctactttttt tatctggttc
    31321 tatcaagttt attttaacaa gattatcttt ttccttaact tctacttcca tgtctaaacc
    31381 attaaaatac tcatttgata atgtatctaa gattaatcgc agtttatcct taagtgaact
    31441 ttcttcatca atctgtaagt actttactat tttctcatcc tttgaaatta tctctatagg
    31501 tattttagta gtttttttag aattaatcat tttactagtt gtttcttctt ttgcatcttc
    31561 atccttcttt acaactgctg attctttaga atcaactagc tcaactgcac tgatgctata
    31621 atccatacat gccgtactca ataaagtagt agcaattatt aaaaaactta ctgctatttt
    31681 attcataagt atccctctcc taattctctt tcgatactta ttataccata gtatagatta
    31741 tatttttata cttttaaatc aatttataaa ttattatagt aaattattta caattctaag
    31801 ctatttgaat ttatttttat agtatattct ttctctaaat tcgacagatt tttattcaaa
    31861 agccctttta tttctctcgt atttctatac ttgttgctat gttcttcact atgttcataa
    31921 tcatatgaat cattgtcata ttcttcatca ttattaaatt cttcaattgc ttcttttgac
    31981 tttatatcac aattaaattt atacttattt actggtatat caagtcttac cttagaattt
    32041 ggaacttcta tgtccaggaa cttagcaatt ggcatatcag agtacaagta aaattcactt
    32101 tcataatttg aatttctacc tattttctct tttatattta cattttcagg tatatactct
    32161 tctatattat tgataaatac atcttcattc tctgaagata tatacaatga ctctgattct
    32221 atgtttacat ttttaacgcc taaaattgat tttgttgaca agtgaagatt gttattagat
    32281 attaagttta ggttttctaa tctatttact ttgttctctg ttatagctct actaaaacct
    32341 ccataagaag ttttatatgt tatagtattt gaagctacat tatcgtatac aaataagtgt
    32401 gagcttatac ttgatgcatt tatgtctaca ttatttggta catatattat aatttctcta
    32461 taatcatgtg aaaacatact atttatagat gtatttaata attggtcgaa atttttaatc
    32521 tttttaattt tattttcata ttttctttca ccttttacaa ctaaagtctt atcttttaaa
    32581 gttacattgt atttgtaaaa ctcattattt cctcttgttg ttacacgcac atcttcactt
    32641 gaacttttct ttattgtaac gtttatattg tcaacagcta tatctaactt gtcaatattt
    32701 actttttctt gataaagttt attttctttt ttaaattcag cttccttttc taaaccaaag
    32761 tttattaggc ttggcataac taaaatccct gaccatgttg tccctattat acctattacc
    32821 aataaaaccg cagcaaatat agcaagtttt ttattcatgt ttatcttctc ctttcttatt
    32881 ttctatatct tcaaggtcct tatatatcgc ctcaaaattt atactatcat cattatattc
    32941 atcagtttct tttatgcttt cttcttctct attgtagtaa tcattattat atgaatcttc
    33001 attaatgtct ttattcaaat cttcatcatt taaagtagct ttttttcttt tttccatctt
    33061 ttcatttgct tttatataaa tattttttct ctttatccaa tgtatataat tatttagtaa
    33121 ttgtttacct agttttatgg caaataataa aatttgccat gcaagtatct caagccctac
    33181 aaatgctatg gttgcgaaaa tacaaaacat cataacatct ggaaccattg ctataaattt
    33241 aatcacaaat ggaattaata taacaaaagc aattaatgag catactaatg caatagtagc
    33301 agatacaata gcacatacaa acgcaaatgc tggaacaatt agaaataaag ataacactac
    33361 taataacccc tttataactt gtctggataa tcctcctaca ccttcttcta tacttggtgt
    33421 aagtttatca tttatatttt cttttgattt attgtacttt tccttagctt catctttcaa
    33481 gatagaataa tcctttttta catttgattt aaatctacta aatttaccat ttaatatgtc
    33541 tgtattcatt atttttccat ctatattttc ttttgattta ctatactttt cttttgcttc
    33601 atcttttaag atagagtaac cttttttcat atctgattta aatttactaa acttatcatt
    33661 caaaacatct gtatttatta ttttttcctc ttcaatattt ttttctttag tttgtaagat
    33721 aagttctttt gctatagatt ttggtgagcc aagtgatgct attatctcca tatcactctt
    33781 tccctctata agaccatcta caaaatactc ttcataatct ctcaatatat cattaacttc
    33841 gctctcagaa aaatcttttt ttagataatc ctttaaaatt tcaagaaact cagttttatt
    33901 caagttcatt acctccctca tcttcactta ttaaaatctc aacagccttt acaaattctc
    33961 tccactcttc catcagattg gataaattct cttttcctag tgaagttatt ctatagtatt
    34021 ttctagcagg accttcatta gattctagta tatatgtttc gaaatacaaa tcttttgtaa
    34081 gtcttcttag aataggatat attgttcctt catttacatc tattacttta gagatgttat
    34141 gtactatctc atatccatac atatctttct tagatatgag agcaagtaca caaatttcta
    34201 aaacaccctt tctaaattgt gtgttcatat actccatcct ttctttaaat acacagtatt
    34261 gtgttttaca aggtactatg ttaaaacttc ttatatcctc ataataacac agtactatgt
    34321 tctgcacaat agttattttt aaatattctt gttgattaaa tccttctata aatgtataat
    34381 actctaatca gatatatttt atgaaagtgg tgataaattg gctaaaatga aagatgataa
    34441 aacaatatta atcctaactg ctcaatttgg tgctggacat ataagtgctg caaaagcagt
    34501 caaagaatgt attattgaaa aatatagtaa ctataatgtt gttatacaaa actttataaa
    34561 tgcaagtatt cccatgatga ataagcctat ggtaaaactt tatgaaaaca atacaaaata
    34621 tactcctgga ttatataatt actattatta ctttaagaaa tcgtttgatt caagacatga
    34681 tttatctcat aaattatata cacctaaact ttctgagtat attgctgata taaatccaga
    34741 tttgattatc tctacatttc cactagctgc tgcttgtgta aataacttta agataaaaaa
    34801 tcctgatata aatataccta cacttacagt tataacagat gttgtagata gtatggaatg
    34861 ggtttttgaa aatacagatt tgtattttgt tccttctcct gaaataaaaa atagattttt
    34921 tcagaaagga ataaatcctg actccataaa ggttactgga gttcctgttg ataagagatt
    34981 tcaaattgaa agtaaagaac tatgttgtga taaatataga ctattacttc taggtggagg
    35041 aagaggtcta tttgatatag atgaggattt tatgcattgg atagatgaat ttattgaaga
    35101 acatagtaat tctatagaaa ttacaatagt tactggtaaa aacaaaaaat tatatgacaa
    35161 tctaactcat aaaaaacctc taaaaaatat taaagtactt ggatttgtaa acgacatgta
    35221 taatctaata agagaatgtg acctaatgct aactaagcct ggaggagcaa ctatatttga
    35281 agctattcaa tcacaaactc cagtactcgt aaaaatgcca aaagttggac aagaaattga
    35341 aaacgctaaa tttataatag ataagggtct tggcatgatt tatagtgatg acctagacct
    35401 taaaaacata ttttataaat tagtatcaaa tgaatttgat tctataataa actttatgaa
    35461 aaaaaattta aaagaattta aaacagtcat tcatccagag aaaattgctg attatatatc
    35521 agaattaata gataatcatt acagttaaat tgtactatta aaaaatttca aacataaaat
    35581 taaaggcaat caagtatgat tttacacttg agaatttact catagtgttt aataatactt
    35641 gattgccttt atcatatcaa aatcaaattg ttatattagt atatattttt cttgtactgc
    35701 atatacagtt gttgtatttc ttccatactt ctctttaatt ctatttgtaa atctgaagca
    35761 gtctggtaat ccttattttc tatagcttct ctcactctag tgaatagtga ttctctagtt
    35821 aaagtatcct tcataatatt gaactttaaa tccgatattg agttccaaac tacctcatca
    35881 agcgcatcca tattttcttt tgtgtgtatc ttgacaatac ttcttatttt ttgaatccca
    35941 gcctctatta ttctagtttt taatttcttt tgccacttat ctatagctcc aatcttaaat
    36001 gattctatta tactatcagt aaatacatca ccttgtttta aagattttaa ttttgaagca
    36061 tatatttcta agtttttcat attttcataa actgtagctg gtggtattcc aaatcttgca
    36121 tttctttctt ccaaactata atgttcaaat acatcatttt catctctata tgctctatct
    36181 ttttctaaat agaaactttc ttcacctaca tttttagata actctttttc aagttctttt
    36241 gtactaagac cactcttagc tgctgctttt ataccatcca acattgtttg ataacatcct
    36301 gctattacta aatacgtgtt agatagaggg cttggtgacc ttaattcaaa tcttacagtc
    36361 tttggatttt tcatatctct tattaatcca acaagcactg acctatttct tgatggaatc
    36421 tcataactat gccctaaaga tgtaactata catacaggag cttcaaatcc aggaactaat
    36481 ctattaaatc catcattaga agctgttaca attgggttta atacttcata attgtaaaga
    36541 agtcccataa gagccccata tcctagttca cttagataat cttcttttaa atcttttgga
    36601 gcaaatagat tttttatact tccatccttt aacttcgcac ttactcctac atgtgtatgt
    36661 ccaccacttc ctgctactcc atgaattggt tttgctttaa atgtaacttc tagtccatgt
    36721 gatgtaaata catcctctat aacatctctt actaataatt cattgtctgc tgcttgtaat
    36781 ggtgtagaaa acttccaaga aacctctaat tgctccatag catggtttgt tcttccatct
    36841 atactaatag aactttgtat tcctccgacc tctttgtgag ccatttcagg gtttacacct
    36901 agcttttgaa gtataattaa acttttctct aaacaagttc ttataatacc atgagttctt
    36961 ttccagtatt gctcttttag gctttgtgat acatataatt tctctaagtc agctttatcc
    37021 tcaggagtat taacccagaa ctcaagttct gtagctgctg ttaacattat ttcctctata
    37081 tcatctactg aatctattcc tatgttattt attacatgtg gatattctct aaatatttca
    37141 tacattgact ttttaaagta cttatctgct ttttgaagta ctcctctaga acatactttt
    37201 ttattatcat gtattaaaaa tgcaggtatc tttaatgttc caacaggtag acctacttct
    37261 tcgtctatat attccatatt gtaatctaca taccaatgac aacttttatc tggcattaaa
    37321 tctactttcg cattatttaa tgtagctata ttataaagct caacacttga accatcagtc
    37381 tgtatagctg attccaaaaa tttatctata tcctccaaaa ataattctac aggaatcttt
    37441 tcatctgttg catttcctcc taaatcaacc cccattaagg aaacaaatct tatattttta
    37501 ttgcttttta ggatttctct taattcttca ttagtgtgtt tatctttctc tattacgtat
    37561 aatagactat ccatttttcc actctcccat aatttttatg tatattttat tttgttaaat
    37621 acaagataaa tttcattttc cttacattta tgaatttttc ttgcaaatta ttcgtgtttt
    37681 attcgtgttt ttatttatat tattatatat caagtataaa aagttcgcaa tattttttat
    37741 tggaaaaaat attatacata caatagttta acatttttac aaatatgggg tatactaaaa
    37801 taaaagtatt tttatttgaa aatttaattt tatttataat ataatctata gtaaaagaaa
    37861 taataaaaaa atttaggaga taaactttat ggaatacata attttcgatt tggaatttaa
    37921 ccaaggattt gataaaaaac tcaataaaac tgtatccaat gaaaagtgtc cttttgaaat
    37981 tatacaaata ggagcaatta aattggattc taaattcaat ataatagata cgtttaatag
    38041 ttatgtaaaa cctacaattt ataaagacat acatcccttt attagtcgta tgactaatat
    38101 taaaaattct gattttaatg attcgcctac tttccctgag gtctacaaca attttataaa
    38161 atttatatca tcacaagacc ctatattatg tgtttgggga gctggagatt taaaggaatt
    38221 atacaggaat atcaactatc acaagctacc atcaaattcc ttacctaaat cttatattaa
    38281 tatacaacaa catgcatcta aatatttcaa taatccagca ggaaaaagca ttggcctaca
    38341 aaatgctatt tctatattag aacttgatga aaaaatgtct tatcacaatg ctttaaatga
    38401 tgcatattat actgctaaag tttttattaa gatatacaat ccaagtatag tcccagatat
    38461 atatctttat acaagtataa aacctaaaac tataagatat tctaacaaaa aaagagttga
    38521 ttatgataag ttatttgatg aatttaggaa aattttaaat cgtgaattaa ctaaagacga
    38581 gaaaaaaatc attaacttag cttataatat ggggaaaaca aatcaattta cattagaaaa
    38641 tgttaagcaa agaaaaaaca aatagagttt atttagataa aaaataggca tgtagattaa
    38701 aaaactagtt aactttaaat ttatatctac agcccaattt ttatcataat taatttttat
    38761 tttatctcta attcaactgg acaatggtct gaacctaata tttcagtgtg tatatccgca
    38821 cttactaatc tatcttctaa ttttttggat gcacaaaaat aatctattct ccaccctgca
    38881 ttattttttc ttgcattaaa tctatatgac caccaactat aaacaccttc tttatcagga
    38941 ttaaaatatc tatatgtgtc tataaaccca ctgtctaata atttagaaaa cttctctctt
    39001 tcttctttag taaaacctgc atttttcata ttatttttag gattttttaa atctatttct
    39061 ttatgtgcaa cgttcatatc tccacacaca attacaggct tatgattatc tagtttggtt
    39121 aaataatcta taaagtcgtc ttcccatcta gttctatatt ctagtctctt taattcagat
    39181 tgagaatttg gcgtatatac tgtaacaaag taaaaatctt caaactctag agttatcact
    39241 ctaccctctt tgtcatgttc ttctatattt attccataca ttacttttaa aggctctttt
    39301 tttgtaaata tagcagtacc tgaataaccc tttctctcag cataattcca atattgaaaa
    39361 tatcctggta aatctaactc tatttgccct tcttgcaatt tagtttcttg taaacaaaat
    39421 atatctgcat ctacttcttt gaaaaagtct aaaaatcctt tcccaacaca agctcttatt
    39481 ccattaacat tccatgatat aaatttcata cttatactcc tcatctaaac attgacacac
    39541 tccccatagc taaagatggg ggcttttcaa caattttatt gtaaattctt aattcacttt
    39601 tattttaaca atgctatact ataatttcat ctaaaattac tatttactta atacctaaaa
    39661 atatatgttt taatcaattt ttaacttttt tgttaaatct tcagggctca ttgaagaaac
    39721 atacatattt gttccaagtt caaatcctcc aaaattatat tttcttggga ttatgtgcat
    39781 atgaacatta aaatacgatt ttgtttcaat ctttgttgga tgagtgtgaa tacataaatt
    39841 aaatggcata tatccattga tattatataa cttcttaaac aacttttcaa atattgtaga
    39901 aagctcctgt atattattat catttaactc accaaactct attttatctt taaatattat
    39961 tcttacttct ccactatact tggttgcata tggtataagt accaaaaaag cttctccatt
    40021 ataaacaact cttctatcta aacttatttc attttctatt aaatcatcat acaaagagct
    40081 tttattgctg tcataatact cctttgaaac caacagttca ttcgtaattt caggtggaat
    40141 aaaagacata gacaatattt gagcatgtgg atgcatcaaa gatgctcctg cctttctcaa
    40201 gaagtttttg aagatactaa catactctac tttatcatct ttcgacaaat ctctaaatct
    40261 atttctatac ataaaaaaaa catcttcaaa ttctttttga ctcatgttat aaaaactacg
    40321 attatgttta tcgctctcta tcattacttc atgaacacca tatacatctc ttgccatatc
    40381 atcaattata ggaaatttat tgtagacaga ttttacaagc catccttctt cactttctat
    40441 ttcaaataat gcatcagttg catgagcttc atttcctttg caaaatgggc attcttcatc
    40501 atattcatta gtttcttcat cttcatttgg gatatctgct gtatctaaag gtctttttaa
    40561 cctatctgta gcaaatataa caacatcatt ggtaattgga tctattctta attctttcat
    40621 aattaacacc tcttagttaa tagcctttaa tataattata tgatatttat cgtatctgtt
    40681 aaataaattt ataatatttt ttataatttt gcatatttat tattatatat tttgtctata
    40741 gctgaaagat tataactaaa aaatcactat cagtatatca gaatattatc ttactataaa
    40801 ttaatttatg aatacataat ttagtggcat aaacatgggt attttactat aatttgttaa
    40861 aagtttattt taaagctata ttaatttttt ttgatgattt ttacccataa gtatgttatt
    40921 attatcttgt aaagatgtct tgtattcaaa catacaattt acataataca atatacgtat
    40981 ttaaattaaa tagggggtat taaaatgaat tattcaaaca gagttagtgc aatgcaagcc
    41041 tctccaatca gaaaacttgt tccatttgca caagcagcaa aagataaagg aatcaaagta
    41101 tatcacttaa acataggaca accagacata aaaactccta aaggattttt tgatgctgtt
    41161 aaaaactttg atagcgaagt cttagaatat gctacatcag aaggtattcc agaattatta
    41221 gaagcacttc aaaattacta taaaacttac gatatgaatt ttgaaaaaga tgaattatta
    41281 gtaactaatg gtggtagtga agctttactt ttcactatga tggcagtttg tgacccagga
    41341 gataatctat tagttccaga accattctat acaaactaca atggatttgg tcaatctgta
    41401 aacgtagaag ttaatgctgt tacaacaaag gcagaaaacg gtttccacct tccatctaaa
    41461 gaagaaatat tgtctaaagt agatgataaa actaaggcta taattctttc taacccagga
    41521 aatccaactg gtgcaattta tacaaaagaa gaacttaata tcctagctga aatagctaaa
    41581 gaaaaagatt tatggataat agcagatgaa gtttatagag aatttgttta tgatgggctt
    41641 gagtatacaa gctgtggtaa cttagagggt gtacaggata gagttataat aatagacagt
    41701 gtttctaaga gatacagtgc ttgtggtgct agaattggtt ctatcgcttg caaaaataaa
    41761 ggtcttatcg ctcaaatcct taaattatgt caaggaagac tttgtgttcc tacattagag
    41821 caaataggag ctgttgagtt atacaaaact cctgtttctt acttcaaaga agttaatgaa
    41881 gagtacaaaa aaagaagaga tgtattatac aatgaattaa tgaaggttga aggtgttata
    41941 tgtaaaaaac caactggtgc tttctatata gttgctaaat taccagttga aaatgcagaa
    42001 gatttcacaa tttggatgtt aaaagaattt aacaaagata atgaaacagt tatggtttgc
    42061 cctgctgaag gattctatgc tactccaggt ttaggacgtg atgaaataag attagcttac
    42121 atcttaaatg aaaaagacct tcatagagct gctacattat taaaagaagg tttagagcaa
    42181 tatgtagctt tacaaaaata attttttaaa cttaatttat ataaatttaa tgatataaaa
    42241 tgtccccttt tcagatatta tttgaaaagg ggacatttct taattaatat attaattaag
    42301 acttagacct gactattttt cattttcttt atatagtgtt ggccagtaat ctttattcac
    42361 tttaattaaa gcatctaaaa cttgtcttgc cttagttgga gagattatag ttctatttaa
    42421 tgttaaagat aaaatagctt tttcataaga ttgttcaaag tatgcatcaa ctgctaactt
    42481 ttcataagct agttgacctt ctaacagtcc cttataaaat gttggtatat tacctacatg
    42541 taaaggtctc ggtccattct ttgtaagaat cgcagctact tctaccattg catcatcagg
    42601 taagttgcta atagccccat tattttctgt tataactatg gctatttggt gtttgttatg
    42661 agctattgct tctgcaactt caactataaa ttccccatga gcatcattat gcactaaatt
    42721 agaacctata gatgttcctt tttctataac agatttacat tcttcaaata cacgcttttc
    42781 tcttccattc ataacttctc ttgctctagt acaatttata tttgatttag ctactttttt
    42841 atcaggatat agataatact gtacatatgt attaggtaag taatctggta catcttccaa
    42901 catatcttta accattccat aagtgtctaa ccaagattta tctctttgtt ctgcatcaac
    42961 aggttggaat ccagagtttg ctactaaatc ttttatctta ggtaccaaat cttcaccatt
    43021 tttatcatac aaatgtgtaa accatccaaa gtgatttaat ccaaagtata caggctcaaa
    43081 tgttcttgaa tccatgtcta acaatcttcc ataagacctt aacaagttaa ctggttggtc
    43141 acacatatta agtaatcttt tatcatctgg aaatactctt tttaatgctt ctgcaacaat
    43201 agcagcagga tttgtataat ttaatatcca tgctttagga gagtattttc ttacagtctt
    43261 aaccatttct atcatatcac ctatagacct taacccataa gcaaatccac cagctccaca
    43321 agtttcttga ccaaccagtc caaattgtag aggaatcttt tcatctaatt ctcgcatctt
    43381 aagaccacca gttctcattt gaacaaatac aaaatctaca tctataaatg cttcatctgg
    43441 gtttgtagta tattcaagtg ttgctttagg atattcttct ttaaataata tttttccaaa
    43501 ttcccctatt aatctttgac gttcttctac tacatctaac atagttactt tatttagagg
    43561 aaatctatca ctcattttac ataaagactt taaaagtcct ggagtccaag tactgcctcc
    43621 tccaactata gctatattgt atcttttttt cattgtcata ttaatcaccc ctgtttttta
    43681 ttaactaact tgtaagatga ttataatata taaagttttc tacatgaata cttttaggta
    43741 atcattaaaa atagttcaat tttagtattt ttttcaaaat tgtaattttt ttcatctatt
    43801 gtatttttca atgtatttat gaaacatata ttccataaga gaaagtgctg gcactctaga
    43861 tgtatagtta acactattaa aattatattg tggagaagat acaaataatt ttatatcagc
    43921 tatatttgat aatgaattga cacttatatt tgtcagagaa attactttaa cttgatttgt
    43981 ttttgctata ttacatgcct taataacttg agcagtctct ccagacatag atacagcaat
    44041 tacaaggtca tctttagtaa tatttgatgc agccaaatac atgagagttg aatcatcata
    44101 gtattttaca tttacaccta tagctgaaaa acgttgagac atttcagtac atactaatct
    44161 agaagaccca acaccaaaaa aatatatatg cttagaatta tgtatttcat ttaaacacat
    44221 atctatattc tctacattta ttaaattttg tgtatccata acatcagtaa aaatatcact
    44281 ttgactttta gtaaactcct tatttctttc atttctattt gaatatgaaa aaactaaaaa
    44341 gtttttaaat tcactatatc cagaaaagcc gagcttctga caaaacctta ctatgtatgc
    44401 agtcgaatta aaagtttcat ttgctatatc tttaatcttt ttttgattta ttttttcaga
    44461 attttttaga aaatagttta aaagagatat ttcatttgta gataattttt tttgatttat
    44521 atctaatttg tctattaggt tcatttatac ctccataata atgatttgta gttaaacaac
    44581 tttctcatat ttattatata aatatacact atacagcatt tatcttatgt ttattatact
    44641 ataaaaaaga caatacaaat tataaattag tattgccttt taatatttac tttaaataga
    44701 cattgcaaaa gaaacttcgt ctcttacttg agatacagat ggcccataaa ttatttggat
    44761 atctttatca gatactttta taactcccat tgcacctgta tattttttaa aagcattttc
    44821 atctacttta ctcatatctt taactactac tcttaagcga gttatacagt ttgttatatc
    44881 ttctatattg tccttaccac ctaaatattt aattatagat aatgctctct tagtagtttc
    44941 tccactttga gctatttcta ctttatttga taaaggtttt aaatcaaaat ttatatctgt
    45001 tatttcttct cgtcctggag tttgtaagtt aaatctaagt atcaaaatct taaatacatt
    45061 ataataaact attgcataca ctaaaccaag tattatagaa taaatccact ttgtatttgg
    45121 ttgcataact ccaaatacta ctaaatctat tatgccacct tgagtatttc ctattgctac
    45181 tccaaatata tgcatcatca taaatgatat acctgataaa aatgcatgta taacaaataa
    45241 tattggtgat acaaataaga atgaaaattc caatggttcc gtaattcctg ttacaaatgt
    45301 tgctaaagca ccagctagta atataccttt aaccttcgac ctattttcag gctttgcagt
    45361 tttatacatg gcatatgcag cagcaggaag accaaatacc attataggta ttttcccttg
    45421 agctaaaaat ctagttgctt ctgttgaaaa actgattgtt tcagggtcac ttagttgagc
    45481 taagaatatg ttcaatgctc caaatacttc ttgccctccc acaactaaag aacctcctac
    45541 ttcagtaaat ctaaacatag catttaatat atgatgtaat ccaaatggta ttaaaagtct
    45601 ttcaagaaat ccaaatacaa atgttcctaa atatcctaaa ctagctatac caatacctac
    45661 actctgaata attgtattta catatggcca tatatatggt attattatcc ccaaaataga
    45721 gaatactact gttgttatta ttggaacaaa cctagttcca ccaaagaatc ctaaataatc
    45781 tggcagtttt atattatagt atttattatg taatgatgct gttattatac caactacaat
    45841 tccaccaaat actcctaatt ctaatgtttg tatgcctaaa gttacagctt gacccatttc
    45901 tctcatcatg tcaggttcag caataatccc cctacaccct aacatataat ttacactagt
    45961 gtgtaatact aaataaccaa caaaaccaga aaatgcagaa actgcttttt cttctttagc
    46021 aagaccaaca gatattgata tagcaaagaa tattgctaag tttccaaaaa gtactcctgt
    46081 tatagctttt gtaaaaccca atattccttg aataaaatta tttgctaaaa aaggcatatc
    46141 actaactata ttaggatttg aaaatgctga acttattcct aaaaatatac ctgctgcaga
    46201 tattaatgct attggcaata aaaatgattt tcctaactgt aaaaagaacc ctttcatttt
    46261 ttgtttcatt tctatccctc catttttaaa tttttaaaac gttttctatg tcataattat
    46321 atatttttat tttgtttttg aaactttata atttgtttta aaatattttt acataaatag
    46381 taattttttc aaaactgtaa attttttcaa acaaagacat agtaacgttg taacatatat
    46441 aaatttaaaa agagcttaga aaataaaatt attgaatata aaatggaaaa atataaatta
    46501 cacaaagaga tatgagtacg tactaacaaa tacgaaaatg tataagaaat tataaaaaag
    46561 tacatcaaga aaatttaaga tgtacttttt agattgtatt tagacttata attatatagt
    46621 taaatgaata acaaagaatt aatcttagta ctattaaata caaaaatttt tatatttttt
    46681 ctatctaaat tcttctttgt ttaatatata ttgaagagta tttattgctt ctaaatattt
    46741 ttttctgtct tcttcagtta atttagaaag tttttctcct aaaccatcac ttaatttatc
    46801 cataaaatca gttgctatct tatctccctc ttcagtaatg tcaattagat attttcttct
    46861 atcaactaaa tctcttttcc ttgttacata cttcttatca gttaaatcat caaccatact
    46921 agttaaactg cctttttcta tatttaactt agagcataaa tcagtcatac ttattactcc
    46981 ataatttttt atgaaaacta atgctcttaa ttgtgtttta tttacattat aatctggtgc
    47041 aaattttttt aggtcttcta agtacaggct agaataaatc ttaggaaatg tttttactaa
    47101 aaaacttatg gtatctttta ctatatagtt catataatca cctcggttgt tttaaaattt
    47161 attaaattat ctaaataact ttatttcagt ttacattaaa aatattgcaa gtacaatagt
    47221 ttttgctgaa tgtttaaatt taattagtaa tcttatcatt tttttatttt tatttatatt
    47281 attcctatta aatattattt atatagctat tttattaaat tcatggtata gtaagtaaag
    47341 aagaactctt cacagcggtg gagagaagac gctagcccat taaaatggct atcttattta
    47401 taaataagat agccgttttt aattatattt tatctgtaca attctatact cttaagtcta
    47461 catctacttt tcctaagtct tccttataat cttttatagc aggttctaca acttcattta
    47521 caaattctac aacttgttct ggagctctac ctataaagtt ttttgggtcc attatagata
    47581 gtatttcttc cttgctcatt ttaaatgaat catcattcat tataagttca attaagttat
    47641 tatccttgcc ttcatgcttt actctatagg cagctttcat agaatattct cttattattt
    47701 catgtaattc ctgtctatct ccacctcttt ttacagcttc cataagtatt gtttctgtag
    47761 ccataaaagg aagttcttca tttacacgtc tatttatcat attttcatat actactaaac
    47821 catcagttac atttatccct atttcaagta tagcatctgc tgccataaat gcttgaggta
    47881 ttgccaatct cttattggct gagtcatcta atgttctctc taaccattga gtggcttgta
    47941 ccattgcagg gcttatagat tcagagatta tatacttaga taaagatgct attctctcac
    48001 ttctcattgg attcctctta taagccattg ctgatgaacc tatttgattt ttttcaaatg
    48061 gctcttctaa ttcttttaaa ctttgtaaaa gtcttatatc attagtcatc ttatgcatac
    48121 tttgagctat ccctgatagt atgcttatta cttgataatc taactttcta gtatatgtct
    48181 gtccacttac tgcataggaa gaatggtatc ccatcttttt acaaattttc ttatctaact
    48241 cttttacttt ttcgtggtct ccttcaaata agcttaaaaa acttgcttga gtaccagtag
    48301 tacctttaac tcctcttaac ttcatgttgt ctattctata gtttaaatct tccaaatcaa
    48361 gaattaaatc ttgagcccat aaacaagctc tttttcctac tgttgtaagt tgagcagctt
    48421 ggaagtgtgt aaatccaaga gctggcatat ctttgtactt aagtgcaaat tcttttaaat
    48481 tgtttattaa atttacaagc tttactctta gaagttttaa agcttcattc atttgtataa
    48541 catctgtatt atctccaaca tatgcagatg ttgctcctaa atgtattatt cctttggctt
    48601 tttcacattg taaaccataa gcttttacgt gagacattac atcatgtctt acttcttttt
    48661 ctatttttct agcttcatca aaatttatat tatctatatt agctcttagc tcatctaatt
    48721 gttcttcagt tatattgatt cctaactcat gttcaccttc tgcaagtgca acccataacc
    48781 ttctccatgt agagaattta aattgtggag aaaatatata actcatgtcc ttgctacaat
    48841 atctgtctgt aagtgggttt gagtaagtag tatatttatt atctatcatt atattagcac
    48901 cttccttaaa tcttttgtcg tgtgattact gtgtacacat tagtacataa ataattatat
    48961 ataattttac gaactataac aagacttttt tattttttgt tcacatttat atatagtatt
    49021 ttttatttat tactattttt tatttatatc caaattatct ttttcaatta agtaatccac
    49081 tatttcacta tcaacaaaat ttaatacata cttatatcta attgcatagt atttatcact
    49141 ccatccaagc tgatttttta catcactttc atctgcccca agccttaaac ttattgctgc
    49201 aaatgaatgt ctaaaatctc ttgctgaata ttgagaaagt ccagctaaat ctaatgcttt
    49261 tttgactata agtctgacat ttctatctgt tatagaatta ctcttttgag ttgtaaaaat
    49321 aaaatcatca ctaggtatta ttacctctgg aagcccagaa taattcctgt aatcttctaa
    49381 aagtttaaaa aagtatggat gtaatttaac tactctttct tttcttccct tacctaacct
    49441 tacatatgag ttatcgtttt catctaccat aaggtctttc cactttaagc taactaattc
    49501 atttaataaa cacccagtag taactagaca gactattatt actctatctc ttatatttaa
    49561 ttttggtagg atacctataa gtttatttat ttcttgtata cttagaacat catcttttgt
    49621 ttttattctt gttactgtgg gtttttctac atatctaaat ggatttatat ctatgtatcc
    49681 ttcttttttt aagaagttat aaaaactatg taaataacta tatatctttt cacaagttga
    49741 tttcgcatat ttagtctgta tatagtctac aaaattttta caatcttctt tcgtagcata
    49801 aatcaattct ttccctttta aaaattcttt aaacagtata acctttgata aataatcgtg
    49861 tttcgtgttt ggatttaatt ttgtagaaaa aacattaaaa gtttcatact cataatcatc
    49921 tagtattttt gtactataca taataactcc tttccttttc ataaaataaa atagctatac
    49981 ttttactata ccatatattt ccccctcaaa tatacccctt attcaataga taacttttaa
    50041 gtttattttg ttatattgtg aaggttttat gaaaatatca atcttccatt gtgcactaca
    50101 cagtactatg gtattataag ttagtaaaag gtggcatttt tttattaatc tatgaataaa
    50161 atatgctact ataaatttgt attggaggta ttttgatttg gaaacattta gtttgcttga
    50221 aatttttaag gcagtcattt tgggcatagt tcagggaata acagaatggc taccagtaag
    50281 tagtacaggg catatgatat tagttgatga gtttataaaa ttgaattttt caaatacatt
    50341 tataagtaca tttttagttg taattcaatt tggttcaata ttagctgtac ttgttatatt
    50401 ttttagaaaa ttaaatcctt ttgatagcgc aaagaatatt aagcagaaaa aagaaacagt
    50461 ccgtttatgg cttaaggtta taattgcagt tataccatct ggagttattg gaatactgtt
    50521 tgaagatgat atagatagat tattctttaa ttcaacagta gttgctattg ctcttattgt
    50581 atatggtata ataatgatag ggcttgaaaa gagaaataaa aaacctaagt ataaagattt
    50641 ttctcaagtt acatataaat tagctctttg tataggatta ttccagtgtc tagctcttat
    50701 accaggtaca tctaggtctg gttctactat aataggtgca gtattacttg gtacttctag
    50761 atatgtagca gcagagtttt cattcttctt agccatccct acaatgcttg gagctagtgc
    50821 attaaaactt ctaaaagctg gatttggatt tactggattt gaatggctta ttttaggtgt
    50881 tggttctgta gtagcatttg ttgtttcaat tgtagttatt aaatttttta tggactatat
    50941 caaaaaacat gacttcaaag tatttggtta ttatagaata gtcttgggta tagtggttct
    51001 tgcttatttc tttctattat aatgtattct aatttaataa caaatgataa ggagaaaagt
    51061 tatgagtgtt aaaaaagaaa tatttgattg gattaagtca atagctatgg ctattgtact
    51121 tgcatttgta attctacaat ttataagacc ttctattgta agtggagaat caatgtatcc
    51181 tactttagat gataaagatt atctgatttt aaataggata tcatacaagg ttggtaaacc
    51241 tgaaaaaggc gatattgtag tttttaaaac caatttagtt gatggagaaa caggaaagaa
    51301 aaaagactta ataaaaagag ttatagctac tgaaggtgac agaataaaaa tatcaaattc
    51361 taaagtgtat gtaaatggaa aattattaaa tgaaccatat atacacaata actatacttc
    51421 tggagatata gatactgttg ttccaaaagg taaactattt gcaatgggag ataatagaga
    51481 aaatagtaat gatagtagat tccctgatgt aggtatggtt gatgaagatg aagttcttgg
    51541 taaggttatg gtgagactat tacctcttga taatattggg aaagtagact aaaaaataag
    51601 agactgaatt ggtaaagttt attttacaaa tctcagtctc tttttatttt cttaaattac
    51661 aaatattttt ttactaaata tcttttgatt ctgtaataag tcttattata tcacttgctt
    51721 cttcattaaa agttgttact atacttaggt agtctgttgc tctactcata gctaaataaa
    51781 ccttatttaa atctcctaca aagtcattta cttgataatc ttgttcaata tcatttatag
    51841 tttgattgta taacatctct aattcacata ctattaccgc tttatactct aaattcttta
    51901 ttgcatatat atttgctatt gtagttccta ccttcttact tatatttgtc aaattatctt
    51961 ctgcacatat ataaggaatt tttgcctctt ccaatgcttt ttttaacata tattgaaagt
    52021 aaatggtttt tccacttttt aatttcttct tattgtaagg ataaacgata gcaacttcag
    52081 aataatcaag acctttttta cttattaaat actcaatttc ccagattaca gaacttattt
    52141 ggtcatcaag gtcacttact tttattatat caacagactt atttccacct ctaagtgctt
    52201 tagtataaaa gaaagtacta aattctgtat ttggtcttaa ttcgttgata tatgagttag
    52261 aattatttga aaatttattt gtaaaatcta caatctcttt agcttgtcta taatttttac
    52321 ttaagataat cttatcatca aattctatac cattatataa ttttttaaat atattcaatc
    52381 tattagagat attcaaggag ttacaagaac acacattaaa tatgaatttt gttttatata
    52441 aaaactccct tataaaatct atttcatact ctaaaaagct ttctgcttca tcaataaaaa
    52501 tagccttgaa catattttta ttttttataa tgttctgtgc ttgttttaca agattattaa
    52561 aggtcttttc gtaatcattt tttaacatgt tataatctac aattaaattg aattttttag
    52621 ctagtttaaa tataaaagaa ctaaatgtgt gaacttctaa attgttatta tctttgtata
    52681 ataactctat cctctctcga agctcattgc acaattgttt tgtatgtgta aatatgacta
    52741 atttatgatg tggatatact tttgctaatt ttatagccct agaaagcata attgctgttt
    52801 tgcctatacc agaaccacct tcaataagtg tattcccata tttaatagaa acaacatctt
    52861 taatttgaac gtcttccatc atagttgctg tatacttata gtcatcattg tagaatgaaa
    52921 tttttttaaa tttttcattt agatgaagtt tatcatttat tatatagtat tctgaacata
    52981 tgtctagtaa aaataaattt aagtttattt catcattctc atcattaaga tattcctcca
    53041 gcgaaccctt attcattata tcttgaagct tatttttatc tattatatta ttattaacaa
    53101 attcttcaaa ttcatatgtt tcctcaactt caacgtatgg cataatgtaa acgtagttat
    53161 aacttatatt tctattcttt tgtcccattt ttagtttcaa aagttcatat tcctcattca
    53221 taacttcaag taactcttct tctaaaataa aaaatatatc ttctgttgta tccataaatt
    53281 ttataaataa aattcttcca tctttaacat acaacaaatc ggtatttatt cctttaaaag
    53341 gagtaacttt tggcactact ttaacgtcct cattgatatt ttcgtaaaat attttctcga
    53401 actccttcaa ttttagggcg tctaactgag aatttatatt tatgtattgg ctcacttaca
    53461 ttacctcctt ctgttatact taattatacc attttgaact atatttttat atatactttt
    53521 tataggcaaa tttagtaaat aaattcaaaa taaatacaaa taaatacaaa actgctaaaa
    53581 aaattttgca ataagtatag atatttattg ttataa
//
LOCUS       DAMJVI010000013        52380 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.013, whole genome shotgun sequence.
ACCESSION   DAMJVI010000013 DAMJVI010000000
VERSION     DAMJVI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 52380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52380
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.013"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..606
                     /locus_tag="PC545_000878"
     CDS             <1..606
                     /locus_tag="PC545_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2455668.1"
                     /translation="ISGYLTKKLQDLNVDTIRTDILTSPTVTDVIVWNIEQSGTDTFS
                     ATYEVDQQIKEGEQTTTVKATYTVKVHVDADRDMVIIQNPTLAPAIEKSDYEPKTPEA
                     DASVDADTVNDATAFLETFFKLYPTATAKELAYYVFGNVLEPIGRDYLYSELVNPVLT
                     KDGDNMKVKVAVKFLDNQTKATQVLQYELILHKDSNWKIIM"
     gene            819..1676
                     /locus_tag="PC545_000879"
     CDS             819..1676
                     /locus_tag="PC545_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2455669.1"
                     /translation="MYKEVGISSNNVPITLSIWYTERSSPTIVFLPGTMSHPLMYENF
                     LCGLSERGFNIIGIHYLSHGKSPKIKKNYTMEDLLHNVYDATTYGIENFGENIAVMGS
                     SQGGILAAMAAGRDTRLKAVFPHNIMLTTLKETMSLTKYPTWSHRFMGLIQWGFRVGG
                     KLLPNYKVPGDAYLDYDRVFYSEQAKQDCLNDPMLLPYYPLRFVTSLFNADLSCLENG
                     AIQCPVILITAKGDPLFSFDYTNKVFDLIHVPQKELLALELNRHMIFNEDTDTTINAL
                     EATLHKYLG"
     gene            1754..2335
                     /locus_tag="PC545_000880"
     CDS             1754..2335
                     /locus_tag="PC545_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2455670.1"
                     /translation="MPRFTEQEKEIINSKLLIEGEKLFALHGLKKVTVDDLVAAVNIS
                     KGSFYAFYPSKEHLYVEINFRLQKELFTSIETTIKKKKYKNHRDLAKDVIMLSLTGVI
                     TSPILSQIDLSLMDYLQRKISSDIFENHMYSDIRILEILEDLGVVFLVPHTVIIKSLY
                     SVLSCLEQFKEDEELNMIQNLLVNGIIQQVVAE"
     gene            2439..3284
                     /locus_tag="PC545_000881"
     CDS             2439..3284
                     /locus_tag="PC545_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455671.1"
                     /translation="MIDVKKLYFSYTDKPFVENVSFHVGRGEIFGFLGPSGAGKSTIQ
                     KVLTGLNTRYKGSVKVAGTEIREHTNQFYENIGVDFEFSTCYEKFTARQNLAYFASLY
                     EKQPRSIDELLHMVGLENDGDKKVADFSKGMRSRLNFIKALVHDPDILFLDEPTSGLD
                     PTNSRLMKDIILAEKKRGKTIIITTHNMFDATELCDQVAFIVAGKVSALDSPHNLIMS
                     RGAAKIQYTYYDKGEKTGECLLDRTTEDKLLKSLISENRLLSIHSSEPTLNDIFVDIT
                     GRTLQ"
     gene            3281..3964
                     /locus_tag="PC545_000882"
     CDS             3281..3964
                     /locus_tag="PC545_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455672.1"
                     /translation="MRLGRLICGDIHFQWKYGFYFIYFILTVLYVCGIAALPEHWKTD
                     IASIMIYSDPAAMGLFFMGAIVLLEKSQKVLNAMVVSPVKISEYILSKTVALIAISTV
                     IALILGVVSGSNHLLGIAVGTALTSAIFTMLGIIAATKISNLNQFLIVIMPIEIICFV
                     PPIVGLFVKLPYLFRFFPFTACMNLITGKSVLLSFDMVLVIATLIILYIVARHTVEHM
                     WKSLGGVKL"
     gene            3961..4659
                     /locus_tag="PC545_000883"
     CDS             3961..4659
                     /locus_tag="PC545_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455673.1"
                     /translation="MKKILSSTIQIFKQIKSDPMMFAACFSPFIMGTLIKFGIPFLER
                     ITKFSLQAYYPIFDLLLSIMAPVLLCFAFAMITLEEIDDKVSRYFSITPLGKSGYLFT
                     RLGVPSIISAVIAFIVLLLFSLEKLSIGMTICLALLGSVQAIIVSLMIITLSSNKLEG
                     MAVTKLAALTLLGIPAPFFIDSYYQFAVGFLPSFWVAKAMQNEAVLYFSIGLVVALVW
                     YYFLTKRLFRKLAG"
     gene            4671..5582
                     /locus_tag="PC545_000884"
     CDS             4671..5582
                     /locus_tag="PC545_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2455674.1"
                     /translation="MEKGRLLQNKNMNKAIQVSKITLIINLSLSLLKFAAGYIGKSSA
                     MLSDAVHSASDVVSTIVVMVGIKISEKQPDREHPYGHERMECVASIILSVALAITGAG
                     IGYSGIKKIFSGQYNTLSVPSGIALTAAVLSIVIKEWMYWFTRSAAKHTNSDALMADA
                     WHHRSDALSSVGSLIGILGARLGYAILDPIASVVICGCILKAALNIFKESINKMVDHS
                     CDNATETRIREVVLQQQGVDGIDELKTRMFGAKMYVDIEILADGNLALYDAHRIAEGV
                     HQTIENNFPQCKHCMVHVNTNELLYSA"
     gene            5634..5774
                     /locus_tag="PC545_000885"
     CDS             5634..5774
                     /locus_tag="PC545_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455675.1"
                     /translation="MEQYWWIIIVVVLIVVGLLKIVIMKKYNQRRNSNNRSKDSEDDK
                     MY"
     gene            6065..6691
                     /locus_tag="PC545_000886"
     CDS             6065..6691
                     /locus_tag="PC545_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2455676.1"
                     /translation="MSDTKENILKIALHLFAVNGYEAVSVSMIAEALGMTKGALYKHY
                     KNKQDIFDCIVERMYQIDNKRAKDYQVPESTIEVTPNAYRNTTIGSLITFTLAQYDFW
                     VTDEFASNFRKMLILEQYRNLKMNMLYQKCLVNGPISYIEDIFNEMIEKGVLQECDTK
                     QLAIEYYAPLFLLISVADAVENCWDLKTQLTTHVKQFVKKYIINEKVK"
     gene            6813..7529
                     /locus_tag="PC545_000887"
     CDS             6813..7529
                     /locus_tag="PC545_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2455677.1"
                     /translation="MEYIRSKKYFTPDLQTKIMGPNPIKLQEELLQNHKIPSGSYVCD
                     LGSGQGLTSVFLAKEYGFVVFAVDLWSEPKENEEFFKKMGLSSAQIKSVKADANELKF
                     EKEFFDAVVSTDSYNYFGRDPIYLDEKLLPFVKKGGFVYITVPGMKKDCHEKLPEELL
                     LSWTPEELDYIHDITYWSNIISHCKKADLISIYEMESNSEVWSDWIKQNNEYAINDRK
                     AIEAGGGDYLNFIAIILQKK"
     gene            7679..8275
                     /locus_tag="PC545_000888"
     CDS             7679..8275
                     /locus_tag="PC545_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDF2455678.1"
                     /translation="MKILWIIIIMADNLLPLLLARFYPNYKHKEMALSVLGSRQSPVK
                     WIYNVWCIISGVVFCIAPYALYQENDSGWAIAIWMLLAIYGIGCEIISGVCPLNENRQ
                     DEDIITKIHGGTSAIGFMALLVVPLLFAILQFQTTKVVLGAISLVCFTIAFIFFCFFI
                     MGEKEKFANTVLRYGGLWQRLVLISCYIPLFVWSVSNQ"
     gene            8362..8553
                     /locus_tag="PC545_000889"
     CDS             8362..8553
                     /locus_tag="PC545_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HDF2455679.1"
                     /translation="MKKYEYVEINYNAKGVVFLCTDKHKEIIDSYAKTGYHYAGFVPT
                     EIDAKGCMRKIDLIFEKED"
     gene            8577..8807
                     /locus_tag="PC545_000890"
     CDS             8577..8807
                     /locus_tag="PC545_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455680.1"
                     /translation="MIGLKRRDMNYIMKVGAKAQGVSVSEFRAMLQDTIDKTMESTDP
                     DVQANFKRYFGNKRPTPEEYIYTITKKTKVKV"
     gene            9074..9493
                     /locus_tag="PC545_000891"
     CDS             9074..9493
                     /locus_tag="PC545_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2455681.1"
                     /translation="MKPSDFQKTIQCQFECKLKKVVKGIIWNYRKELKRRKNKEISFS
                     ELPDITVESLATWDDYETNYTIFNVCGIDIKVLDDELAEALKQLPEKKRNNVLMYYFL
                     EMSDTEIADIMNYTRGAVFKHRHRALETMKEILKEKK"
     gene            9496..9750
                     /locus_tag="PC545_000892"
     CDS             9496..9750
                     /locus_tag="PC545_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2455682.1"
                     /translation="MKKKYKHPSYYLISSAMDGDETAIEKLLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGRIREALIKMILDFEIDIDIETEEE"
     gene            10125..10328
                     /locus_tag="PC545_000893"
     CDS             10125..10328
                     /locus_tag="PC545_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2455683.1"
                     /translation="MNNTDVPIWEKYTLSIEEASRYFRIGEKKLRKLAEENLDSGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            10406..11596
                     /locus_tag="PC545_000894"
     CDS             10406..11596
                     /locus_tag="PC545_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2455684.1"
                     /translation="MSKMKRQDSKGRNLRLGESQRKDGRYVYKYTDIFGKPQFVYAWK
                     LVPTDKTPTGKRDDKSLREKEKQIKKDLDDGIDTIGGKMTVCQLYAKKNNQKKNIKRN
                     TEIGRQYLMNALENDPLGMRAIDTVKQSDAKEWAIRMNDKGYSYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFHFKLSDVLEDDTEPKVILTPEQEEKLLSFMETDNVYSKYRDEVI
                     LLLETGLRISELCGLTTHIDMLNRVINIDHQLLRDTEVGYYISTPKTKNGKRELPLTE
                     RAYQALERILKNRGKAQPLVVDGYSNFLFLNREGLPKVAGNYESMVRGLIKKYNKTHE
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTFQSAKAELE
                     RLAS"
     gene            complement(11853..12653)
                     /locus_tag="PC545_000895"
     CDS             complement(11853..12653)
                     /locus_tag="PC545_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455685.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYAFSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGATKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(12848..14446)
                     /locus_tag="PC545_000896"
     CDS             complement(12848..14446)
                     /locus_tag="PC545_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2455686.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGVKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFSYLVCYRLDRISRNVSDFSTLIEKLNELSISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEIVKLIYNKYLELRSLSKLYKYVYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILDYLKNSGMDVVGTPDRKCGILTYAKNTSNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRISTGKTALLSGLIKCGKCGSNMRITYKGK
                     KTDKDLKYYYICGTKKTLGSDSCNCKNLNGPLVESLVIDKIKNCKEESIISAFNSNNI
                     KTDNVFNIFKSETNLLKNQILDKERLIKNLVIELAKNTGSTASDYIVSQIESLNNEIS
                     ILKDKINILESSNINISSDSINIDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSSLGNLNIVYMGAEVDNSVSLSN"
     gene            14774..14950
                     /locus_tag="PC545_000897"
     CDS             14774..14950
                     /locus_tag="PC545_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455687.1"
                     /translation="MEINVIEIFPKDKAKLNKIEMDKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            15124..16254
                     /locus_tag="PC545_000898"
     CDS             15124..16254
                     /locus_tag="PC545_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HDF2455688.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLINSTYQVFEEIVPDPPIEVVVAGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSATAKKYAQIAELVGLGSANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILKNIK"
     gene            16495..16845
                     /locus_tag="PC545_000899"
     CDS             16495..16845
                     /locus_tag="PC545_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2455689.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            16853..17302
                     /locus_tag="PC545_000900"
     CDS             16853..17302
                     /locus_tag="PC545_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HDF2455690.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELKYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKYKNIDEV"
     gene            17404..17979
                     /locus_tag="PC545_000901"
     CDS             17404..17979
                     /locus_tag="PC545_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2455691.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENIAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            17972..19381
                     /locus_tag="PC545_000902"
     CDS             17972..19381
                     /locus_tag="PC545_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HDF2455692.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     VLSKFDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGKLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFENIL
                     MDENYDETNEVSIGNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGGYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFS
                     DREHGTIQILINKDNDYRNIAIIDDGIGFDSNNINKNSLGTYIIDSYITQMLKGNIER
                     KSSENGTEVLITIPTQINI"
     gene            19565..20998
                     /gene="eutA"
                     /locus_tag="PC545_000903"
     CDS             19565..20998
                     /gene="eutA"
                     /locus_tag="PC545_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDF2455693.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKEDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVTDTGCLDIGGRLVKIDKISKKITYISPKIDKIIKDNNLNLQVGTMATVENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISIDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCEYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYEKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            21016..22380
                     /locus_tag="PC545_000904"
     CDS             21016..22380
                     /locus_tag="PC545_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HDF2455694.1"
                     /translation="MILNTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWLLD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIKVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKHG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            22393..23274
                     /gene="eutC"
                     /locus_tag="PC545_000905"
     CDS             22393..23274
                     /gene="eutC"
                     /locus_tag="PC545_000905"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HDF2455695.1"
                     /translation="MNEKDLKALVEQLVGQMLGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRAGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            23294..23947
                     /gene="eutL"
                     /locus_tag="PC545_000906"
     CDS             23294..23947
                     /gene="eutL"
                     /locus_tag="PC545_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDF2455696.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            23958..24659
                     /locus_tag="PC545_000907"
     CDS             23958..24659
                     /locus_tag="PC545_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2455697.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRVDKVGAGIVTLT
                     ICGDVGAVKAALEAGEIAASRVGTLRTSHIIPRIHNEVTNALFKTKEPKVCEVSEDKE
                     KNFEITLEIADNVSKNLSEKENVILDVNENSGDTENLYSDIDKTNKKIETVDDSLTEA
                     NEEKIENTNDKEVLEQVKIENSDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLKK
                     EELINLVKKLSRRDR"
     gene            24662..26131
                     /locus_tag="PC545_000908"
     CDS             24662..26131
                     /locus_tag="PC545_000908"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HDF2455698.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQEKLSKMSQEQIDKIVKAVV
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIIGEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISE
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESERLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHVNDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSMDKEELINLLVQKLVKELA"
     gene            26227..26514
                     /locus_tag="PC545_000909"
     CDS             26227..26514
                     /locus_tag="PC545_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2455699.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGATAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            26641..27402
                     /locus_tag="PC545_000910"
     CDS             26641..27402
                     /locus_tag="PC545_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HDF2455700.1"
                     /translation="MGVLTENEVKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKKYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKVQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLDEKLGDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            27415..28044
                     /locus_tag="PC545_000911"
     CDS             27415..28044
                     /locus_tag="PC545_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2455701.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGE
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGSTLGL
                     KAPIKQSGDLEGTLSIKISTQYGEVELDKGLMVAKRHIHMTPKDAEKFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKA"
     gene            28086..28811
                     /locus_tag="PC545_000912"
     CDS             28086..28811
                     /locus_tag="PC545_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HDF2455702.1"
                     /translation="MNYDNLVDLITEEIYKKINSNEIKISNKPKAVIVFEQDNNKFNL
                     LKDGFEIVEFDKSIRECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMNGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFMSFEDEICKFGVEIIKDLNVITKNKLNIKKELESTR
                     GSKKEDTKVLDKDILNLTSVESVKIDNEFSLDLRNKKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKL"
     gene            28824..29096
                     /locus_tag="PC545_000913"
     CDS             28824..29096
                     /locus_tag="PC545_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HDF2455703.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKDTYKEGFMVAA
                     DNAGAGNGDLVLISKGSSARESIEKSHVPVDATIVGVVDSFEVFDE"
     gene            29089..29640
                     /locus_tag="PC545_000914"
     CDS             29089..29640
                     /locus_tag="PC545_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HDF2455704.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     IVGGETSYINECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDSIVSIGVVE
                     SSKICAGIKMLDKTLKSGDLVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESENVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            29652..30743
                     /gene="eutH"
                     /locus_tag="PC545_000915"
     CDS             29652..30743
                     /gene="eutH"
                     /locus_tag="PC545_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDF2455705.1"
                     /translation="MSINEIIIYVMVVFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEVMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGLAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            30745..31218
                     /locus_tag="PC545_000916"
     CDS             30745..31218
                     /locus_tag="PC545_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HDF2455706.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            31731..32252
                     /locus_tag="PC545_000917"
     CDS             31731..32252
                     /locus_tag="PC545_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2455707.1"
                     /translation="MEEIVKKEQASMNDIFLYLKAKSNSLYKFVILYNEFINKPKDYG
                     TGELINMVEVHMLTAIEENPGISPTQLAKIWNRTKGAISQTISKLEKKGYITKEKKEG
                     NAKTILLYPTTSGVELSKAHKLYDTIDVTRTFEYLIKECTLEEIDSFYKVIDLFINLL
                     EESPQENNDSEKV"
     gene            32631..33806
                     /locus_tag="PC545_000918"
     CDS             32631..33806
                     /locus_tag="PC545_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2455708.1"
                     /translation="MNLKQLSEKYEEYVIQMRREFHKYPELSFKEIRTTQRIREELQK
                     IGVPFVSIEPNIVIATIQGKNEGKTIAIRGDIDALPMQEDSDVEYKSEYDGIMHSCGH
                     DAHGAMLLGLAHMLNDIRKDLKGRVLLVFQAAEEVGGGHKEIIQYFKENGGPDRLIAT
                     HVWSDIEAGKISVEPGPRMAGGSGWRIDITGRGGHGSRPDQSIDPIKPACDIVLKVSS
                     IPVNHISVLEQCVVHACAIHGGTTIGNVIPNNAEVIGGYRYFTEESGKKVFDIIKQMS
                     NGVGQVYGVDVKVTHTGGIGPVINDEEAVKMAKEIVSNIDGLELDSYEPICASDCYGE
                     ILKEYSGFYCYLGVGNKEKGIIYAQHHPKYNIDEDILKLGCEFMAKYAVEFLNKNNN"
     gene            33840..35378
                     /locus_tag="PC545_000919"
     CDS             33840..35378
                     /locus_tag="PC545_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2455709.1"
                     /translation="MQGALKENKKKSGGGFLGWIEKVGNKMPHPLALFTYITLIVVAL
                     SAIASFLGFSATSPATGKLIKVMNLISAKGLVLFMQEFVKNFQNFPVLGVVLVMAVAT
                     GVCEKTGFFSTAIKMSLSKVKGNAVVFIIAFIGVFANQAGDAAFVLVPTLAGAIFYGL
                     NRNPLAGIFCGFASVGGGFATAVIPGGWDVTLTPITVSAAQTIQPAFDMTLLASYYAL
                     FVSAFIVATVSTIVTVKFIEPKLGKYTGKPEGIDDNQGFEVTKEQAKAAKLAGFTVLA
                     YVALLIALCIPANSFLRSPEGSLIFGAPLMSCLQFFIVILFFLPGIVYGMRVGKIKTV
                     KDLNDILCQSMAAMAPFIVLAIVIGQFLTLFSVSNLAQVLAIKGGALLNSLPLPPQAI
                     ILLFLVLVAFINIFVISGSTKWMIFGPVFVPMLMQLNIHPAFTQFVYRLGDSVTNHLS
                     PLNAFFIILLATVQKYDKNAGMGTIFSAMIPYTIGYFVVLAVLVVVWMTIGIPVGIGG
                     QVWL"
     gene            35410..36612
                     /locus_tag="PC545_000920"
     CDS             35410..36612
                     /locus_tag="PC545_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2455710.1"
                     /translation="METTEIRYNFDEVIDRSGTNCLKYDLKSQFMPESPEDALPLWVA
                     DMDFACAPEIINAMHERIDRKIFGYSSQQTDDYYEAVCGWFKRRFDWKIEKENIFFSP
                     GVVPALGFFTQILTQKGDGIIIEKPVYYPFEDKITNNERKVVNSPLKFEDGHYSMDYD
                     GFEELAKDENNKVFILCSPHNPVGRVWKKEELRKIVDICKKYDLWIISDEIHCDIIRK
                     GEKHIPLEVACPDYKDRIITCTAPSKSFNLAGMQLSNIVINNKELQEKWMLETDSKLA
                     IFLPNPFAIVATKVAYNDCEDWMNQVNEYIDGNIKYVEDFVNEYMPKVKIIKSQGTYL
                     VWLDFRGYGLDAKQLEYVMLNKAKVLLDEGYIFGEEGAGFERINVASPRTIIEECMNR
                     IKCAFEGL"
     gene            complement(36779..37378)
                     /locus_tag="PC545_000921"
     CDS             complement(36779..37378)
                     /locus_tag="PC545_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2455711.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGEARCIKVQL"
     gene            37548..38906
                     /locus_tag="PC545_000922"
     CDS             37548..38906
                     /locus_tag="PC545_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455712.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKVRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERMDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHENKISQNQKILLDEKFTLDDSIKNLSSIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFKDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            38899..39531
                     /locus_tag="PC545_000923"
     CDS             38899..39531
                     /locus_tag="PC545_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDF2455713.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNASVEETFKKSGIPVKVDKSNGQVTFGEKTMFDVDSDVLKPEAKEML
                     KMFVPKYIETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            39860..41017
                     /locus_tag="PC545_000924"
     CDS             39860..41017
                     /locus_tag="PC545_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2455714.1"
                     /translation="MIEISIKNKKNSKIKTLILLLIVSISIYLTGCTTLDNGNNQSNN
                     NDSLSSSISSDKNVKIHFIDTGNSDAILIQDGKTFILIDGGDNDDEGLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALANKGLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILP
                     KLEKVDLLKVAHHGSRSSSSQEFLDKVNPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNSNTSKNDSFKNKQNT
                     SNSEEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYGK"
     gene            complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC545_000925"
     CDS             complement(41164..41799)
                     /gene="lexA"
                     /locus_tag="PC545_000925"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDF2455715.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLRVKGESMINAGILDEDYVVVDKKNTALNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(41903..43030)
                     /locus_tag="PC545_000926"
     CDS             complement(41903..43030)
                     /locus_tag="PC545_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2455716.1"
                     /translation="MNNDKKIIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNADCVKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRDNGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            complement(43351..44196)
                     /locus_tag="PC545_000927"
     CDS             complement(43351..44196)
                     /locus_tag="PC545_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455717.1"
                     /translation="MIYIIEILKISVINIFCFIGIFIVFGLIFSITENLNNKFIFSSF
                     GKTGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTIMIILIFKILLPDSYMKVTQNIDLKLYVSMINNLNING
                     FIHIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFVISSITYLFGFDNLNISSLIVKYNIFISFFMAIGFIFSIVTLIISYILSLVSP
                     FKNFS"
     gene            complement(44226..44597)
                     /locus_tag="PC545_000928"
     CDS             complement(44226..44597)
                     /locus_tag="PC545_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2455718.1"
                     /translation="MKLKLLVEEYAVCRLNNDSKIPTWIDTEKFYSITRTDDELSVVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILSCNGYEVE"
     gene            complement(44750..45010)
                     /locus_tag="PC545_000929"
     CDS             complement(44750..45010)
                     /locus_tag="PC545_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HDF2455719.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGVAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            complement(45199..46143)
                     /locus_tag="PC545_000930"
     CDS             complement(45199..46143)
                     /locus_tag="PC545_000930"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HDF2455720.1"
                     /translation="MIENNSEKIKVLENDIKQLITISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSAYEKVTLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSMVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYKKFRNIGKCL"
     gene            complement(46164..47018)
                     /locus_tag="PC545_000931"
     CDS             complement(46164..47018)
                     /locus_tag="PC545_000931"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDF2455721.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKLLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC545_000932"
     CDS             complement(47033..48394)
                     /gene="accC"
                     /locus_tag="PC545_000932"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDF2455722.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEDNNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC545_000933"
     CDS             complement(48410..48862)
                     /gene="accB"
                     /locus_tag="PC545_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDF2455723.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQSTSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADSFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            complement(49132..49578)
                     /locus_tag="PC545_000934"
     CDS             complement(49132..49578)
                     /locus_tag="PC545_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455724.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILITVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKENDTNLKESEV"
     gene            49853..50131
                     /locus_tag="PC545_000935"
     CDS             49853..50131
                     /locus_tag="PC545_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455725.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            complement(50260..51006)
                     /locus_tag="PC545_000936"
     CDS             complement(50260..51006)
                     /locus_tag="PC545_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455726.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDDVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VDIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLNFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILSDKNDLNTSQEVLITVNVK"
     gene            complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC545_000937"
     CDS             complement(51135..51779)
                     /gene="trmB"
                     /locus_tag="PC545_000937"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDF2455727.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            complement(51917..52237)
                     /locus_tag="PC545_000938"
     CDS             complement(51917..52237)
                     /locus_tag="PC545_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HDF2455728.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
BASE COUNT        19388 a         7230 c         8680 g        17082 t
ORIGIN      
        1 atcagcggtt atctaacaaa gaaattgcag gacttgaatg ttgatactat tagaaccgat
       61 atactgacta gccccacagt tacagatgta attgtgtgga atatcgaaca gtcgggaacg
      121 gacacttttt ctgctaccta cgaagtagat cagcagataa aagagggaga acagacaaca
      181 acagtcaagg caacctatac ggtaaaagtc catgtagacg ctgacaggga tatggtaatt
      241 attcagaacc ctacccttgc accagcaatc gaaaaatcgg actatgagcc aaagacacca
      301 gaagcagacg caagtgtaga cgctgatacc gtgaatgacg ctaccgcatt tctggaaaca
      361 ttctttaagc tgtacccgac agccacagca aaagagcttg cctattatgt atttggaaat
      421 gtgcttgaac ctatcggcag ggactacctt tattcagaat tggtaaaccc agtacttaca
      481 aaggacgggg ataatatgaa agtcaaggta gctgtaaaat tccttgataa tcagacaaag
      541 gcgacgcagg tattgcagta tgagcttatt ctacataagg atagtaactg gaaaattatt
      601 atgtaagaca ttttgttggc aggatctatt ttcggatata gtctgccttt tctgtagcaa
      661 atgtactgca ctgacaagct atttcattgt attttaagca attaaacctt tcttgacaat
      721 tttatcttaa atcatatatt tatggatatt gactttctgc ttgcaataag ctataatatg
      781 actataaaat caaatcagtc atataggagg tatgtggtat gtataaagaa gttggtattt
      841 cttcaaataa tgttccgata acactttcta tctggtatac agaaagaagt agtcctacca
      901 ttgttttttt gccgggtact atgtcacacc ctctcatgta tgagaatttt ctatgtggtt
      961 tgagtgaaag aggatttaat ataataggca ttcactatct ttcacacgga aaaagcccta
     1021 aaatcaaaaa aaattataca atggaagatt tgctgcataa tgtatatgac gcaacaactt
     1081 acggaataga aaatttcggt gaaaacatag ctgtcatggg ttccagtcaa ggcgggattt
     1141 tagctgcaat ggcggcaggt agggacacaa ggttaaaagc cgtttttcca cataatatca
     1201 tgctgaccac attaaaagaa actatgagct taacaaaata tcccacatgg tcgcaccgtt
     1261 ttatggggct gatacagtgg ggctttcgtg taggtggaaa attgctgcct aactacaagg
     1321 ttccaggaga cgcttattta gactatgaca gagtatttta tagtgaacag gcaaaacaag
     1381 attgcttaaa tgaccctatg cttttaccct attatccttt aagatttgtc accagcttat
     1441 ttaatgctga cttatcgtgc ctggagaatg gggctataca atgtcctgtt attttaatta
     1501 ctgccaaagg cgacccgctt tttagctttg attataccaa caaagtattt gatttaattc
     1561 atgtaccgca aaaggaatta cttgctttag agcttaatcg tcacatgatt tttaatgagg
     1621 acacggacac tacaataaac gcattggaag ctaccttgca taaatatctt ggatagcata
     1681 ttgattatat aatcagtaaa tgttatactt ttcatgacga tataaatatt ttagtcataa
     1741 ggaggttatg agtttgccac gatttacgga acaagaaaaa gaaatcataa acagtaaact
     1801 tttgatagag ggagaaaagc tgtttgcatt acacggctta aaaaaagtaa cagttgatga
     1861 tttagtagct gctgtaaaca tatcaaaagg ttctttctat gctttttatc ccagtaaaga
     1921 acatctatat gttgaaatca attttcgctt acaaaaagag ttatttacaa gtatagaaac
     1981 aaccattaaa aagaaaaagt ataagaacca tagagattta gcaaaggacg ttatcatgct
     2041 aagtttaacg ggggtgatta cttcgcctat tctttcacag attgatttat ctttaatgga
     2101 ctatctgcaa agaaaaatat cgtcagatat ttttgaaaat catatgtaca gtgatattcg
     2161 tatattggag atattggaag atttaggggt tgtatttttg gttccgcata cggttattat
     2221 aaaatccctg tattctgtct tatcctgttt ggaacaattc aaagaggacg aagaactgaa
     2281 tatgatacaa aatcttttag tgaatggtat tattcagcaa gtagtagcag agtaacaatt
     2341 ttgtttggtt atttgacagg caggtagcca aacaaatttt ttacagttta tatgactata
     2401 tatctataaa cgtcataaag ttaaaggagg aatgaataat gattgatgta aaaaaactgt
     2461 attttagcta tactgacaaa ccgtttgtgg agaatgtaag tttccatgtt gggagaggag
     2521 aaatttttgg ttttttagga ccctctggtg ctggaaaatc aactattcaa aaagttttaa
     2581 ctgggcttaa tacaagatat aaaggcagtg taaaagttgc aggtacagaa ataagggagc
     2641 atacaaatca gttttatgaa aatattgggg tagactttga attttcaacc tgctacgaga
     2701 aattcacagc acgacaaaat ctcgcctatt ttgcttctct atatgaaaag caacctcggt
     2761 ctattgatga attattgcat atggtaggct tggaaaatga cggggacaaa aaagttgctg
     2821 acttttccaa aggtatgcgt tctcgcttga atttcattaa agcgttagtc catgaccctg
     2881 atatattatt ccttgatgaa cctacaagcg gtcttgaccc tacaaatagt cgtttaatga
     2941 aagatattat tcttgcggaa aagaaacgcg gaaaaacgat tatcattact actcacaata
     3001 tgtttgacgc aacagagcta tgtgaccaag tagcctttat cgttgccgga aaagtcagtg
     3061 cattggatag cccgcataat ttgattatgt caaggggagc tgccaaaata caatatacct
     3121 actatgataa aggcgagaaa accggagaat gtctgcttga tagaaccaca gaagataagt
     3181 tgttaaaaag tttaatttcc gaaaaccgtt tgctttctat tcatagtagt gagcctacat
     3241 taaatgatat ttttgttgat attacaggga ggacacttca atgagattgg gacgtttaat
     3301 ttgtggtgat atacatttcc aatggaaata tggtttctat tttatctact ttatattaac
     3361 agttttatat gtgtgcggga ttgccgcttt gccggaacat tggaaaacgg atattgcttc
     3421 tattatgatc tactctgacc cggcagcaat gggattattt tttatgggag cgattgtcct
     3481 cttagaaaaa agtcaaaagg tgttaaatgc tatggtggta tcccctgtaa aaatatcgga
     3541 atatatactt tcaaaaacag ttgcgttgat tgctatttct acggtaatcg cattgatatt
     3601 aggggtagtt tccggcagca atcatttact gggtatcgca gttggtacgg ctttgacctc
     3661 agcaatcttt actatgcttg gaattattgc agctacaaaa atttcaaact tgaaccaatt
     3721 tctcattgtg attatgccga tagaaattat ttgctttgta ccacctattg ttggtctgtt
     3781 tgtgaagtta ccatatctgt tccgcttttt tccattcaca gcttgcatga accttataac
     3841 tggaaaaagt gttttactat cgtttgatat ggtacttgta attgctacgt tgattatcct
     3901 ctacatagtt gctcgacata cagttgagca tatgtggaaa agtttgggag gtgtaaagct
     3961 atgaaaaaga tattatccag caccatacag atatttaagc aaatcaaaag tgaccctatg
     4021 atgtttgcgg cttgcttctc cccttttatt atggggactt taattaagtt tggtattcca
     4081 tttcttgaaa gaataacgaa gttttcttta caagcgtatt atccgatttt tgatttatta
     4141 ctttctatca tggctcctgt attgctctgc tttgcatttg caatgattac attggaggaa
     4201 atcgacgata aagtgtcacg gtacttttcg attacccctc ttggtaagtc ggggtatctc
     4261 tttacaaggt tgggagtacc ctcaattatt tcggcggtca tcgcttttat tgtactgtta
     4321 cttttttcat tagaaaagtt gtccattgga atgacaatat gtttggcgct tctcggctca
     4381 gtacaggcaa ttattgtttc acttatgatt atcacattat caagcaataa attagagggt
     4441 atggcagtta caaaactagc tgcacttaca ttgctcggaa taccagcccc cttttttatt
     4501 gatagttact accagttcgc ggttggcttc cttccatcat tttgggtagc gaaagccatg
     4561 cagaatgaag cggttcttta tttctccata ggattggtgg tagctttagt ctggtactac
     4621 ttccttacaa aacgtctgtt tcggaagctg gcaggataag gaggcatatt atggaaaaag
     4681 gtagacttct gcaaaacaaa aatatgaata aggctattca ggtttccaaa attacactaa
     4741 tcatcaatct gagtttatcg ctacttaaat ttgctgccgg atatatagga aagtcaagtg
     4801 ccatgctgtc ggacgctgta cattcggctt ctgatgtagt aagcacaatc gttgtgatgg
     4861 ttggaattaa aatatcagaa aaacagcctg atagagaaca tccttatggg catgaacgta
     4921 tggaatgtgt ggcttccatt attctctctg ttgcattggc tataacgggt gctggaatag
     4981 gatattcagg tatcaagaaa atcttttcag gacagtacaa tacgctttct gtccctagtg
     5041 gaatcgcact tacggcagca gtcttatcta ttgttataaa agagtggatg tactggttta
     5101 caagaagtgc tgcaaagcat acaaattctg acgcacttat ggctgacgca tggcaccacc
     5161 gctctgatgc actctcgtcg gttggttcct taattggcat tttaggagcg cgtttaggct
     5221 atgctattct cgatcctatt gcaagcgttg tgatttgcgg ttgtatctta aaagctgctt
     5281 tgaacatttt caaagaaagc attaacaaaa tggtagacca tagttgtgac aatgctactg
     5341 aaacaaggat tagggaagtt gtgcttcagc agcaaggggt tgatggtatt gatgaattaa
     5401 aaacccgcat gtttggagcg aaaatgtatg tagatattga aatattagct gatggtaatt
     5461 tagctcttta tgacgctcat agaattgctg agggagtcca tcaaacgatt gaaaacaact
     5521 ttccacaatg taagcattgt atggtacatg ttaatacgaa tgaacttctc tattctgcat
     5581 aggacaggga agatgattta aggctattga aagggaaagg agggaagtag gttatggaac
     5641 aatactggtg gattatcatt gttgtggttt tgattgtcgt tggcttactt aaaattgtaa
     5701 ttatgaagaa gtataaccaa aggagaaatt ctaacaatcg ttcaaaagat agcgaggacg
     5761 acaagatgta ttaaggttta ttgaaatctg ttatgttgta gaatgatgtt tttagatact
     5821 agaaagtgct ttaagggggg ctaacgttaa ggaaagcttt gagtacaatt ctttacaatc
     5881 aacagtcttt tgcatagaga aatgtatgta ggaaatatgg tgcaaggcac aaaacaccag
     5941 caattgagaa atcaacctat gaaccaaagg cacactacat tttatactac ttgacaagta
     6001 aacgaacatt tactttaatg gtttaaagaa gtaaacgttc gtttactaaa gaaggaggtt
     6061 gtaaatgtct gacacaaaag aaaatatatt aaaaattgca ctacacttat ttgcagtgaa
     6121 tggttatgag gctgtttctg ttagtatgat tgcggaagcc ttgggaatga caaaaggagc
     6181 attatataaa cattataaaa ataagcaaga tatttttgac tgtatcgtag aacgtatgta
     6241 tcaaattgat aataagcgag ctaaggatta tcaagtacca gaaagcacaa tagaggtaac
     6301 cccaaatgca tacagaaata caactattgg aagtctaata acatttactc ttgcacaata
     6361 cgatttttgg gtgactgatg aatttgcgtc taattttcgc aaaatgttaa tactagagca
     6421 atatagaaat ttaaaaatga atatgcttta tcaaaaatgt cttgtaaatg gtcctatcag
     6481 ttatatagaa gatatattta atgaaatgat tgaaaaaggt gttcttcaag agtgtgatac
     6541 aaaacagctt gctattgaat attatgcgcc attatttcta ttgataagtg tagcagacgc
     6601 agttgaaaat tgttgggatt taaaaactca attaactaca cacgttaaac aatttgtcaa
     6661 gaaatatatt ataaatgaga aagtaaaata atcctttatt ctgtcctaaa tataaaccta
     6721 actttttaat ttcagcagag aaactcaaaa tgaatattgt tataagtcac aaatattaac
     6781 tattaaaata ttacaataac ggagatgaga aaatggaata tattcgcagt aaaaaatatt
     6841 ttacacccga cttacagaca aaaattatgg ggccgaatcc tataaaatta caagaggaac
     6901 tattacaaaa tcataaaatc ccgtctggta gctatgtctg tgaccttgga agtggacaag
     6961 gtttaacttc tgtatttctt gcaaaagaat atggatttgt tgtttttgca gttgacttat
     7021 ggagtgagcc aaaagaaaat gaagaatttt tcaaaaaaat gggattatct tctgcacaaa
     7081 ttaaatctgt aaaagctgac gccaatgaac ttaaatttga gaaagagttt tttgatgctg
     7141 tagtaagtac agattcatat aattattttg gacgtgaccc tatatattta gatgaaaaat
     7201 tgcttccatt tgttaagaaa ggtgggtttg tttatatcac agttcctggc atgaagaaag
     7261 attgccatga gaaacttcca gaagagttat tgttatcatg gacaccagaa gaacttgatt
     7321 atatacatga tataacatat tggtctaaca taattagtca ttgtaaaaag gcagatttaa
     7381 tttcaatata tgaaatggaa agtaattcgg aagtttggtc ggattggata aagcaaaata
     7441 atgaatatgc aataaatgac cgtaaggcaa tagaagctgg cggtggagat tatctgaatt
     7501 ttattgctat aattctacaa aaaaaataaa ttctttagaa ccaaacacta taatttgcgt
     7561 tctaatatgt ttaccgattg acatcaataa ttttatcttt gagggtaatg atttgaaaat
     7621 agaagaatac aagaagatac gaaattgata aattagaatt tgatgggatg gtgatattat
     7681 gaaaatatta tggatcatta ttattatggc agataatctg ctgccgttac tgcttgctcg
     7741 tttctatcca aactataagc ataaagaaat ggcactatct gtccttggct ctcgtcaaag
     7801 cccggtgaaa tggatatata atgtgtggtg cattatatca ggtgtggtat tttgtatcgc
     7861 tccatatgcg ttgtatcaag agaacgatag cggatgggca atcgctatat ggatgcttct
     7921 tgctatatac ggcatcgggt gtgaaatcat ttccggagtg tgcccgttaa atgaaaatag
     7981 acaagacgaa gatataatta caaaaattca cggcggcaca tcggcaatcg gatttatggc
     8041 cttgcttgtc gttcctttgc tctttgcaat tctgcaattc caaacaacaa aagtagtgtt
     8101 aggcgcaatt tcactggtgt gctttacaat agcattcata ttcttctgct tttttattat
     8161 gggtgagaag gaaaagtttg caaatactgt tttgagatat ggcggtcttt ggcaaaggct
     8221 tgttttaata agttgttata taccgttatt tgtttggtct gtttctaatc aataaaattt
     8281 agtttatagg aataacaaat tccagtttgt aaaagtcaat aaatccaaag ataaatttgt
     8341 atttgaaagg agttttgaag aatgaaaaaa tatgagtatg tagaaattaa ctataatgcg
     8401 aaaggagttg tattcctttg tactgataaa cacaaagaaa ttattgacag ttacgcaaaa
     8461 acggggtatc actacgcagg gtttgtaccc acagaaattg acgcaaaagg ctgtatgcga
     8521 aagatagatt taatttttga aaaagaagat taacttattt tagggagttg gttctaatga
     8581 taggacttaa aagacgagat atgaattata ttatgaaagt tggtgctaaa gctcaaggtg
     8641 tttctgtatc tgaatttagg gctatgctac aagataccat agacaaaact atggaaagta
     8701 cagacccaga cgtgcaggca aattttaaac gatactttgg aaataagcgc ccaacaccag
     8761 aggaatatat ttatacgatt accaaaaaga caaaggttaa ggtttaaatg ttgtaaaggg
     8821 ggcttgccgt atgaaacgct ttatgtacaa ttcagtatat tcaagcccaa tttaacagca
     8881 tactatttac aattaaatac gctataccag tagctcgtac aaaatttata ttttgtgcgg
     8941 gcttttttaa tacccgaaaa gtttttttaa ttattttcat ttttttggaa gcaaatcaca
     9001 ccttgctgta cagatatggt gcggaaagag ggataaacac ttttcaattc atagctggaa
     9061 aggaggtgag attatgaaac cgtctgactt ccaaaaaaca attcagtgtc agtttgaatg
     9121 taagctgaaa aaagttgtca aaggtattat ctggaactac cgtaaagaat taaaacgaag
     9181 aaaaaacaag gaaatatctt ttagcgaatt accagacatt acggtagaaa gtctggcaac
     9241 atgggacgac tacgaaacaa actacacaat ttttaatgtg tgtggcatag atattaaagt
     9301 ccttgatgat gaacttgcag aagctctcaa acagctaccc gaaaaaaaac gtaacaatgt
     9361 attgatgtat tacttcttgg aaatgagtga tacagagatt gcagatatta tgaattatac
     9421 cagaggggct gtgttcaaac acagacaccg tgcactagaa actatgaaag agatactaaa
     9481 ggagaagaaa tagaaatgaa gaaaaagtac aagcacccgt cttattattt gatttcatca
     9541 gctatggatg gggatgaaac tgcgattgag aaattactgg cattctatga cccgtacata
     9601 tcaaagtgtt gtttacgtcc tttatatgat gaatacggca atgtttatat tgttgttgat
     9661 atggaattaa agggacgtat tcgagaagcc ttaatcaaaa tgattttaga ctttgagatt
     9721 gatatagata tagaaaccga agaagaataa gcaaatacca gcagggcgtg attggttaaa
     9781 ttcccttctt tcctgccctg ctcctgcttt tatacatgga agcctacggc tttcgccgat
     9841 tgttctttga caactgaata aagcagacag atacgtttgt gatacagcga gccacgggga
     9901 ctgtacgcca tgacctttcc gacaagaaag cgagcgaccc acgcagttga tccgagagcg
     9961 acttgtggaa tggtcgtttt gccatgacct gctatcacag aataatgata cgtccgcatg
    10021 gtgcggttct gcccacaaga atgggaatag ttgagatact aacggagctt tccaaagccg
    10081 tctgtctgct tatgataaga cacacagaaa ggagttgttg caacatgaac aatactgatg
    10141 tgccgatttg ggaaaaatac acccttagta tagaagaagc gtcccgatat tttcgtatcg
    10201 gggaaaagaa gttgcgtaaa cttgccgagg aaaatctgga ctctggttgg gttatcgtta
    10261 atggaaaccg tattcagata aagcgaaaac agtttgaaaa aattattgat acactggacg
    10321 aaatctgatg tcttagagcc gtagatatgg tataatatta gtagcgtatc acggctcatt
    10381 ccatgacgga aaggagcttt acactatgtc aaaaatgaaa agacaagaca gtaaaggacg
    10441 caatttgcgt cttggagaga gccagagaaa agacggaaga tacgtttata aatataccga
    10501 tatattcggg aagccacagt ttgtatatgc ttggaaactt gtaccgacag acaaaacacc
    10561 tactggaaaa cgtgatgata aatcattgag ggaaaaagaa aagcagataa aaaaagacct
    10621 tgacgacggt atcgacacta tcggcggtaa aatgacagtc tgccagcttt atgcaaagaa
    10681 gaacaaccag aaaaagaaca tcaagagaaa cacggaaata ggacgacagt atcttatgaa
    10741 cgcactagaa aatgacccat taggtatgag ggcgattgat acagtcaaac agtccgacgc
    10801 taaagaatgg gctatcagaa tgaatgacaa aggttattcc tataagacaa tagataacta
    10861 taagcgttcc ttaaaggctt cattctatat ggcgatacaa gacgactgta tcagaaaaaa
    10921 tccttttcat ttcaagctaa gtgatgttct ggaagatgat acagagccta aagttatctt
    10981 gacaccagag caggaagaaa aacttctttc ctttatggaa acggataatg tttacagcaa
    11041 gtatcgtgat gaagttattc ttctgctgga aacgggactt cgtatctctg aattatgcgg
    11101 actgacaaca cacattgata tgcttaatcg tgtgattaac atagaccacc agctattacg
    11161 tgatacagag gtgggctact atatttccac acctaagaca aaaaacggca agcgagagct
    11221 tccattgaca gaaagagcat atcaggcact tgaacgaatt ttaaagaacc gaggaaaagc
    11281 tcaaccgctt gttgtagacg gttatagcaa tttcttgttc ttgaaccgtg agggcttgcc
    11341 aaaggtagca ggaaattatg aaagtatggt gcgtgggctg attaagaagt acaacaagac
    11401 ccatgaggac aagttgccga acatcacgcc ccactcattc agacatacct actgtacgaa
    11461 catggcaaat aagggaatga accctaacac cctgcaatac atcatgggac acgcaaacat
    11521 aaccatgaca ttagggtatt atgctcacgg tactttccag tctgcaaagg cagagttgga
    11581 aaggctggct tcttaatatc gaaaatgtat cttactactt atttactact tttggttgca
    11641 ttttcatgct ggtaaatgcc agcttatgcg aggtatcttc caaaaagaaa atgcccatac
    11701 agcccgaaaa tacgggatat atcggcattt accaacttat gaaaagataa tcagaaatta
    11761 gtaagttttt atatcaaaaa aatgattact gtaaagaaat cattttttat tataatcact
    11821 gatacatcaa atattctaaa taaaattttt atttatctgg aattgtatat tttttctata
    11881 atattaacta cagcgtacat tgcccaagca cagatagcca atacaaatac tcccatcatt
    11941 actaaatcta atttaaatac ttgagaacca tatactatca agtagcctat tccatatctt
    12001 gaaactaaaa attctccaac tattacacct acccatgcca taccaatatt aattttagtt
    12061 aaatttatta aatttcctgt gtttgcaggt aatatgagtt taaataatat ttgagattta
    12121 gtagctccaa aactttttaa catcttaatt ttttcttcat ctatattcat aaaataatta
    12181 taagctgaaa gtatagtaac aacaacagaa atcgttacag ctgttacgac gattccttct
    12241 attccagcac ctacccaaac tataattata ggagctaaag cagtcttagg aagagcattt
    12301 aaaactacca aaaatgggtc taatattttt gataattttt ctgaccacca aagagcaatt
    12361 gctaccaaaa tacctaatac agtacctata acaagaccaa gaacagtttc atatacagat
    12421 attccaatat gtttaaataa ttgaccacta aatgcatatt gaataaataa attgtaaata
    12481 tcacttggtt tgctaaatag aaatgtattt ataacattta agtcagctaa aagttcccag
    12541 attacaataa aaccaattaa aattaatagc tggtagaata atacatttct tttttctctt
    12601 tttacacctt taacatagtt tatatatcct tcagatcttt tattaagcct catgtgtatc
    12661 tagctccttc cataatatgt cgaaatactt ttgaaaatca ggaactttac gtgttttgag
    12721 aggcgttttt gttccatcca tgttaagttc aatatcgtaa atatttttta tggtagctgg
    12781 tcttttagat agtattgcaa ccttatctga tatgcttatt gcttctgcta tagctaaaaa
    12841 agtgcaacta attgcttaaa gaaacagaat tatcaacttc agcacccata taaacaatat
    12901 taagatttcc taaactgcta tcccaaacga tactattaat aatagtagat aataacaatc
    12961 tcttatcatc taaactggca ttatcaactt catcattaaa tctcttcaaa ttactaacaa
    13021 taatatcaat attaatactg tcactactaa tattgatgtt actagattcc aaaatattaa
    13081 tcttatcctt taaaatacta atctcattat ttaggctctc aatctgggaa actatataat
    13141 cagaagcagt actaccagta ttttttgcaa gctctataac taaattttta ataagtcttt
    13201 ccttatctaa aatttgattt ttaagtaaat tagtctcaga tttaaaaata ttaaaaacat
    13261 tatcagtctt aatgttatta ctattgaatg cacttataat agactcttct ttacaattct
    13321 taattttatc tataactaaa ctttcaacta atggaccatt aagattttta cagttgcaac
    13381 tatcagaccc aagtgttttt ttagtaccac aaatataata atactttaaa tccttgtcag
    13441 tttttttgcc tttataggta attctcatat tagaaccaca tttaccacat tttataagac
    13501 cagataaaag agcagttttt ccagttgata tccttggagt tttatcacga tttttatcta
    13561 attgattttg tacttcaagc catatttcag gttctatgac tccatggtgt tttgcaattg
    13621 ctgctattga gttagatgta tttttagcat aggtaaggat tccacatttt ctatctggag
    13681 tacctacaac atccatacca ctatttttaa ggtaatctaa aatttcatca ttagctttta
    13741 catatgctgg atttctaagt attctagata aagaactagg gtcaaatttg ccaccacgag
    13801 gaccccttat tccattatta tagacatatt tatataactt agataaggac ctaagttcta
    13861 aatatttatt gtatattagc tttacaatct ccatttgttc atcatttact tgaagcttat
    13921 acatacttct ttcttttaga ttttcatcta agaatgtgat tttttcactg ttgaatccat
    13981 gaggtgtatt gcctccaagc catctaccac tacgggctaa ttcatacata ttgtctttta
    14041 tacgttctgc tattgtttct ctttctaatt gagcaaatac acttgatata aacatcatag
    14101 ctcttcccat tggagttgaa gtatcaaatt gttctttgat agatataaat gatatgctta
    14161 gttcatttaa tttttctatt aaagtagaga agtcagatac atttctcgat atcctatcta
    14221 atctataaca aactaggtaa ctaaatttct tatctttggc atcctgcatc attttcttaa
    14281 attgtggtct atctatattg ctaccactaa atccctcatc ttcatatatc aaaaaatctt
    14341 ttacaccaat agactttgca taatcaatac ataattgtat ttgattttct atagaatcac
    14401 cttttccagt aaatcttgat tttcttgaat aaattgcagc tttcaaaatt aaaaccccct
    14461 ataagttagg atatatattt gaaactattt atctacaacc ttttaagtaa acttattaga
    14521 atgaatttat taattaggta aatttatata attctacttt ttataatata tgactattta
    14581 ttttatttct ttactattca agttctaaat caataataaa ttttgatatt taattgtatc
    14641 aggacaaatc tctaatttca tagattatta atataaaatc ataggttagt gtgaaaaagt
    14701 tgaaatttaa atattttaaa agataatttt tttgaactaa taggtataac aaaaatttat
    14761 agggggattg catatggaaa tcaatgttat agaaattttc cctaaagata aagctaaact
    14821 taataaaata gaaatggata aagctagttg gtttgtaaat ataataagta aaaaatatcc
    14881 taaagaagct ttaaatgaag catttagtac tttagaaaaa gaattaaata taagtaaagc
    14941 taatacataa gctttgatta ttgattttat atactattgg ttttatacgc tatgacttga
    15001 cattatatta atcaaatagt atatttttaa atatagcaca atgaagtgtt aaaagagcaa
    15061 tggagctcca tagataaagt ctatggagct tttttgcgta tttaacttaa ggaggatagg
    15121 actttgaata actttgagat taaaactaga ataaaatttg gagaaggctc actaaaagct
    15181 ttgcaagaat taaaaaacaa aaatgtacta ataataacag acccttttat ggtgaaatca
    15241 aaaacaatag ataaaataac atctaattta ataaactcaa catatcaagt ttttgaagaa
    15301 atagtaccag acccaccaat agaagtagta gtagcaggaa tagaagcatt taaatctata
    15361 aatccagacg taataatagc attaggtggt ggttctgcaa tagatgcagc aaaatctata
    15421 atggactttt ctaaaaaaat attaaaaatt aacgaagtag agtttatagc aataccaaca
    15481 acaagtggaa caggttcaga agtaacatca tttgctgtaa taacagatag tcaaaaaaaa
    15541 tgtaagtatc cattagtgag tgatgattta cttccagata tggcaatact agacccagaa
    15601 cttgtaaaaa ctgtaccaaa ttttataaca gcagatacag ggatggatgt attgacacat
    15661 gctatagaag cttacgtatc tacaaattct aatgactttt cagatgcact agctgaaaaa
    15721 gctataaagt tagtatttaa atacttaata aaagcatata aagatggaaa tgacatagaa
    15781 gctagagaaa aaatgcacaa tgcatcttgt ttagcaggta tggcatttaa ccaagcatca
    15841 cttggcataa atcattctat tgcacatgta ttaggtggaa aattccatat accacatgga
    15901 agaacaaact ctatattact tcctcatgta atagaatata attcatcaat agtaggatat
    15961 actaacacaa cttactctgc tacagctaaa aaatatgcac aaatagcaga attagtagga
    16021 cttgggagtg ctaatataag aatatctgtt agaaatttag taaatgaaat aaagaaacta
    16081 caaaaagaaa tgaatatgcc aactaaattg acacaatgca aattaagttt agaagatata
    16141 actaatgaag aaagagaaat agcaaaactt gctataaaag atacttgtac attaactaat
    16201 cctaaggtac caactgaaga tgatataata aacatattaa agaacattaa ataactacat
    16261 tttattacat gtaaatataa tattatacaa atagggaaaa tgtttttaaa aatataaatt
    16321 atatattgac aaatataaaa cataaaacta taatgaaagt atcacaaagg cgtgatactt
    16381 tagtagaaag caatggagct tcttaggaat attaatatta ttcttatgga gctcctttta
    16441 ttttaatcaa atagcttttt aataaactat attcaaagaa agcagggaat aaatttgact
    16501 gaagaatcta agcaaagagt tattcaagaa tatgttcctg gaaaacaggt tacattagca
    16561 catatcatag ctaaccctaa tgaagatata tataagaagt taggattagt acttgataaa
    16621 aaagatgcaa taggaatact tacaataaca ccaagtgaag cgtcaataat agcagcagat
    16681 gttgctacaa aggcttcaaa tgtatcttta ggttttatag atagatttag tggttcagta
    16741 gtaatatcag gtgatgtaag ttctgtagaa tcagcgttaa atgatgtttt agaagtctta
    16801 ggaaatatgt taaacttctc atctacaaaa atcacaagga cataataata caatgaagaa
    16861 tataatattt atgggaaaga caggtagtgg taaaactacc ttatgtcaaa aactagacca
    16921 gttagaatta aagtataaaa aaactcaatc agtggaactt tataataatg caatagacac
    16981 tcctggtgag tatatggaaa atagaaacct atataatgcc ttaataacaa catcagcaga
    17041 tgctaaaatt atagcaatag tgtatgatgc cacacaagaa gaaaattata tagcaccagg
    17101 ttttgccagt atgttttgca aagacgtaat aggaattata actaaaataa ataaagctac
    17161 tgaaaacgaa gtcaatatag cttatgaaag actatcacta gcaggagcaa gtcaaatctt
    17221 taaaatagac acagttgata atattggact tgaaaaatta tttaaatatc tagaaaaata
    17281 taaaaatata gatgaagttt aaggggaaat atttttttat tcaaaaatat tattaaagta
    17341 tgataaaata aaaaaatgta taaactattt taagaagatt taatctttaa gcgaggtagg
    17401 aacatgggga aaaaaatact attggttgat gatgaaccac tgttaaggat ggatacaaaa
    17461 gatatattag aaagtcatgg ctatgaaata ataggcgaag caagtgatgg atttgaagct
    17521 gttgaaatgt gtaaaaaaca caaacctgat ttagttataa tggatataga gatgccaata
    17581 cttgatggta taaaggcagg caaaataata tgtaaagaaa atatagctgg tggagtactt
    17641 ttattaactt catttgaaga taaagaatac gtagaaaagg ctaaaagtat aggtgcttat
    17701 ggatatttag taaaaacagc aagtgaaaaa tcacttatcc caaccataga aatgtgttta
    17761 aataaggtaa atgaatttga aaaaatgaaa aaagatttag aaaaagtaaa ttctaaattg
    17821 aatgaaagaa aattagtaga aagagcaaaa ggtatactta taagagagtt taagatttct
    17881 gaggatgaag catacactag aataagaaaa ttaagtatgg ataaaagaac tacaatgatg
    17941 gaaattgcca aaatgattat aataggatac gatgactaat acgataaaaa aaatatcaac
    18001 tgcacaaaca tacttaaata ataacgaaat tgaaaaaatt atagaagtat caaaatcctt
    18061 agatttaatg gcttcttttt atgaagctga tgtttttata gatgtactaa gtaaatttga
    18121 tgatgaagca attgttgtgg cacatggtag acctaaagaa aagtcaatgt actgtgaaaa
    18181 tgttataggt aaaaaggctt taattgaaaa tgaaccagga gttataaagt gcttaaaaac
    18241 aggagaaaga gtaagagata ttaaagcttt aacacaggaa tataaactgg tcaaacaaaa
    18301 tattcagcca ataacactag atgacaaagt tttagcagta ctaatagttg aaaaagatat
    18361 aagtagtgaa gttaagaatg agtttaatgc tagtgaagac gaagaaaata aaaatttaca
    18421 tcagtttata aaacttattg gaaataatga ctttttagtt gataatttaa attcagccat
    18481 attaatattt gataaaaatg gaaaattaaa aattaaaaat cgtattgcaa ctgagatata
    18541 caaaaatctt gggtttggat atgatataca agatatgcat tacaatgatt tagatataga
    18601 aaccaacacc tttgaaaata tattaatgga tgaaaattat gatgagacaa atgaagtttc
    18661 aataggtaat atgtttttta aaataaagac catatacatc aaagatgatg aatttaaggt
    18721 ttgcaaaata gtacaagata taagtgattt aaagagaaaa gaagaagaat tagttttgaa
    18781 aacagtggct ataagagaag ctcatcatag agtaaaaaat aatcttcata cagtaatatc
    18841 tataataaaa aaacaaagta gattatctca aaatgatgaa gtaaagcagt gtttagataa
    18901 cataataaat agggtatttg caatactttc gactcattat ttactatcta aagaagttga
    18961 taataatata tctatacgag aaggtatcaa tttattagtg tcaaatattc aaggtggcta
    19021 tttatttagt aaggatatag atatacgtat aactggtgaa gattttaaaa ttagtggaga
    19081 aaaagcaaca gcaatacttt tggttataaa tgaaatacta caaaattgtt atgaccatgc
    19141 attttcggat agagaacatg gtactataca aatattaata aataaagata acgactatag
    19201 aaatattgct attatagatg atggaattgg atttgatagt aataatataa ataaaaatag
    19261 cttagggaca tatataatag atagttatat aacacagatg ttaaaaggaa atatagagag
    19321 aaaaagtagt gagaatggta cagaagtatt aattactata cctacacaaa ttaatattta
    19381 aataataact catctacaaa gtagtagttt taattgagca aagaggctta aggttattaa
    19441 tagattaagt taattgaatg tatttataaa aattataagt atatattcaa tttaacaggt
    19501 atttattata actttaagcc tctttgcttg tttaagacat acttaaattt taggaggtga
    19561 gaaaatgaga gaagagttat taagtgttgg tattgacatt ggaacaagta ctactcagtt
    19621 aattttttct aaattaatag tagaaaatac tgaatcaaat ttttcggtac ctagaataag
    19681 tatagtagac aaagaaatta tttataaaag caaagtttat ttcacccctc ttatttcaaa
    19741 taatgaaata aatggtcaac aaataaaaga tatagttgaa aatgaatata aaaaagcaaa
    19801 tatatctaaa gaagatatac atacaggagc agtaataata actggtgaaa cagctagaaa
    19861 agaaaatgca aatgatgtac ttcatacttt gagtggattt gctggggact tcgttgttgc
    19921 aacagcagga cctgatttag aaagtataat atcaggaaaa ggagctggag ctcatattta
    19981 ttcaaaagaa cattcaacta caattgtaaa tatagacata ggtgggggaa ctagtaacat
    20041 agcctcattt aaaaggggag aagtaaccga tactgggtgt ttggatatag gtggaaggct
    20101 tgtaaagata gataaaataa gtaaaaaaat aacttatata tcacctaaaa tagataaaat
    20161 cataaaagat aataatttaa atcttcaagt aggtacaatg gctactgtag aaaatacaaa
    20221 gccaatagtg gatataatgg ttgacttatt aatggaaagc ataggactaa agaaacagac
    20281 acctttgttt gattttataa taaccaataa atcaataagt atagatgaaa atattaaaaa
    20341 tgtttcattc tctggtggag tagctgatta tatttactat gatggagaaa taaaagatta
    20401 ttttgaatat ggtgacttgg gaattttact tgggcaagca ataaaaaatt gtgaatacct
    20461 taaaaaagta aatgtagtta aatctatgga aacaataaga gccacagtgg ttggagcagg
    20521 ttctcatact actgaagtaa gtggaagtac aataacttac actagcgaaa agtttccact
    20581 taagaacttg ccagttttaa aattatcaat tgatgaagaa acaggtactt caaaacaaat
    20641 cagtgaagct ataaaaaata aacttaattg gtttaaacta gaaaatgatt tacagaaagt
    20701 tgctatagcc atgataggaa agaaaaatcc aaccttttta gatatacaag agtatgctaa
    20761 agggtttgta gatggaatgc aacaaatcat agaaaatgat ttacctttta tagtaattgt
    20821 tgaaaatgat atggcaaaag tgttaggtca agcaatatat gcattattaa attatgaaaa
    20881 agaaattgtt tgtatagaca gtgtcaaggt tgaaaatgga gactatatcg acattggaaa
    20941 acctatagtt gaaggtaaag ttttacctgt ggtaataaaa acattagttt ttaattaata
    21001 gaaggaggta agtaaatgat tttaaataca aaattatttg gacgtactta tcagtttaaa
    21061 ggcttatatg atgtaatggc taaagctaat gaagaaaaat caggagataa attagcaggt
    21121 cttgcagcag aatcagctga agaaagaata gcagcaaaag ttgttttatc acacataaaa
    21181 ttggaggata taagaaataa tcctgcagtg gcatatgaag aagatgaagt aacaagaata
    21241 atacaagatg ctgtaaatga aaaagtttat gaagaaataa aaaattggac agtgtctgaa
    21301 cttagagaat ggctattgga ttcaaatact acaagtcttg atataagaag aatatcaaga
    21361 ggattaactt ctgaaatgat agcagcagtt gcaaaattaa tgtcaaatat ggacttagtt
    21421 tatgcagcta aaaagataaa ggttactgct cactgtaata caactatagg agaacaagga
    21481 acattatcag caagacttca acctaatcat ccaacagacg acccagatgg aatacttgca
    21541 tcacttcttg aaggtttaac ttttggtata ggagatgcag tacttggatt aaatccagtt
    21601 gatgactctg ttgagagtgt tactaaagta ttaaaaagat ttgaagaagt aaaacaaaaa
    21661 tatcaaatac caactcaaac atgtgtattg gcacacgtta ctacacaaat ggaagcaata
    21721 agaaatggtg ctcctgcaga tttaatattc caatctatag caggttctga aaaaggaaat
    21781 gaagcatttg gatttaatgg cgacactata gaagaagcta gacagttagc cttaaaacac
    21841 ggtacagcta caggacctaa cgttatgtat tttgaaactg gacaaggttc agaattatct
    21901 tcagaagcac atcatggaat agaccaagtt actatggaag ctagatgcta tggatttgct
    21961 aaaaagtatg aaccattctt agtaaataca gttgttgggt ttataggacc tgagtactta
    22021 tatgatgcta gagaagttgt aagagcagga cttgaagacc actttatggg taaactacat
    22081 ggcttaccaa tgggtgttga tgtttgttac actaaccata tgaaggctga ccaaaatgat
    22141 atggaaaact tagccatact attaacaaca gcaggatgta cgtattttat gggaatacct
    22201 catggagatg atgttatgct taactatcaa acaacaggat accatgaaac agcatcactt
    22261 agagaaatgt ttggactaac agcaataaaa gaatttgatg cttggatgga aaagatggga
    22321 ttctctaaag atggtaagtt aacagagttg gcgggagacg catctgtatt attaggatag
    22381 gaggtattta atatgaatga gaaggattta aaagcattgg tagaacaatt agttggacaa
    22441 atgcttggag aattagatac taatgttgtg tctgagactg ttaaaaaggc aactgaagtt
    22501 gtagtagaca acaatgcttg tatagatgat ataacagaag ttgatataag aaagcaatta
    22561 ctagtaaaaa atcctaagga tgcagaagct tatttagata tgaaagcaaa aacacctgca
    22621 agattaggta taggaagagc tggtactaga tataaaacag aaacagtttt aagatttaga
    22681 gcagaccatg ctgctgcaca agatgctgta ttctcttatg tagatgaaga atttataaaa
    22741 gaaaataata tgtttgcagt ggaaacttta tgtaaagata aagatgaata cttaacaaga
    22801 ccagatttgg gaagaaaatt ttctccagaa actataaata atataaaatc taaatttggt
    22861 acaaatcaaa aggttttaat attagttgga gatgggctta gctcagctgc aatagaggca
    22921 aatttaaaag actgtgtgcc agctataaaa cagggtctaa aaatgtatgg tatagattca
    22981 agtgaaattt tatttgttaa acattgtaga gttggtgcta tggaccactt aggtgaagaa
    23041 ttaggttgtg aagttatatg tatgttggtt ggagaaagac caggattagt tactgctgaa
    23101 tcaatgtcag catatatagc ttataagcca tatataggaa tggcagaagc aaaaagaaca
    23161 gttatatcaa acattcacaa aggtggaaca actgcagttg aagcaggtgc tcatatagct
    23221 gaacttataa aaactatgtt agataaaaaa gcttcaggaa tagaccttaa ataaagtcga
    23281 gggagtgtag aatatgaaaa atgatgtaat tcgtcctaat atactaggaa ttaaaataat
    23341 ttcaaatata agtccagaaa tggcacaaaa actagaatta aaatcacatc acaaaagctt
    23401 aggtttaata actgcagact gtgatgatgt tacgtataca gctcttgacg aagcaactaa
    23461 agcttcagaa gttgatgttg tatatgcaaa atcaatgtat gcaggtgctg caaatgcttc
    23521 aacaaaactt gctggtgaag tattaggaat aatagcaggt ccaagcccag cagaagtaag
    23581 aagtggtcta aatgcagtag ttgattttct agagtatgga gcaactttta taagtgcaaa
    23641 tgatgatgat tcaatagcat actatgctca ctgcgtatca agaacaggta catacctttc
    23701 agaggtagca ggaataagag aaggtgaagc attagcttat ttagtagcac caccacttga
    23761 agcaatgtat gcactagatg cagcaatgaa agctgcagat gttaagatgt gtgaattatt
    23821 tgcacctcca actgaaacaa actttggtgg agcattactt acaggttctc aatcggcttg
    23881 taaagcagca tgtgatgcat ttgcagaagc agtaaaatca gttgcagaca atccaacagg
    23941 attttaagga tagttagatg gatgctatag gcttaataga agtaatagga tatgttgctg
    24001 caatagaagc ttctgatgct tgtttaaaat ctgcaaatgt taatattgta agagttgata
    24061 aggttggggc aggtattgtt actttaacaa tatgtggaga tgtcggtgct gtaaaagctg
    24121 ctttagaagc gggtgaaata gcagcttcca gagttggtac attaagaaca tcacatatta
    24181 taccgagaat ccataatgaa gtaacaaatg ctttattcaa aactaaagaa cctaaagtct
    24241 gtgaagtttc agaagataaa gaaaaaaact ttgagattac attggaaata gctgataatg
    24301 tatcgaaaaa tttatcagaa aaagaaaatg taattttaga tgtcaatgaa aacagtggag
    24361 atactgaaaa tttatatagt gatatagata aaacaaacaa aaaaattgaa acagttgatg
    24421 atagtttaac agaagctaat gaggaaaaaa tagaaaacac taatgataaa gaagtattgg
    24481 aacaagttaa aatagaaaat tccgattttg atagagatga cctatcaaag aacagtgtaa
    24541 aaaaacttaa agctatggct aaaaaattag attcttcaat aacttataaa gacttaaatg
    24601 ttcttaaaaa agaagaatta ataaacttag ttaaaaagct tagcagaagg gatagatagt
    24661 tatgacactt ttagataaag atttagtatc tatacaagaa gtaagaactc ttgtaagaag
    24721 atctaaagtg gctcaagaga aactttctaa aatgagtcaa gagcaaatag ataaaatagt
    24781 aaaagctgtt gtagatgcag catttaataa tgctactaaa ttagctaaaa tggcaaatga
    24841 agaaactgga tttggaaaat gggaagataa agtaattaaa aatacttttg catctatggg
    24901 agtttatgaa actttaaaga acacaaaaac tattggaata attggtgaag ataaagaagc
    24961 caatacgata gatatagcta caccagttgg agttgttgca ggtcttatac cttcaacaaa
    25021 cccaacatca acagtaatat acaaaactat aatatctcta aaagcaggaa atactatagt
    25081 tttctctcca catccaaatg caaaaaactg catacttgaa acagttaata taataagtga
    25141 ggctgctata aaggctggag ctccagagga ttcaatatta tgtataacta atccaagtat
    25201 agttggaacc aatgaattga tgaaacataa agatacaaac ttaatactag caactggagg
    25261 agaagcaatg gttagagctg cttactcttc aggaacacca gctattggag ttggaccagg
    25321 aaatggacca gcatttatag ataaatcagc aaatgttaaa ttagcaatca aaagaataat
    25381 agattctaaa acttttgata atggtgttat atgtgcatca gaacaatcta tagtaataga
    25441 aaaatctatg gaagaaactg ttttaaaaga gcttaaaaat caaggtgcat atgttttaaa
    25501 tgaagaggaa agtgaaagat tagcaaaatt catattaaga gctaatggta ctatgaatcc
    25561 acaaatagtt ggtaaaagtg ttgaggtaat agcaaattta gcagacttaa caaatgtacc
    25621 taaaaataca aaagttctag ttgcaagaga aaataaagtt ggtcacaatg tagcctattc
    25681 aagagaaaaa cttacaccaa tactagcgtt ctatgttgaa gaaaatgtag aagctattat
    25741 gaacagatgc agagaaatat tgttaaatga aggtgcaggt catacattct caatgcatgt
    25801 aaatgatgaa gaattggtaa aaagatttgc ccttaatatg ccagtatcaa gaatacttgt
    25861 gaatactcct tcagcacttg gaggaatagg tgcatcaaca aaccttgtac cagcactaac
    25921 acttggttgt ggtgcaatag gtggaagttc aacttcaaac aatataggac cacttgattt
    25981 aataaatgtt aaaagagtag cttatggaat caaagaaata gaagaacttg gaagagataa
    26041 atctcaagtt gaaaaatcaa tgtttgactc aatggataaa gaagaattaa taaatcttct
    26101 tgttcaaaaa ctagtcaaag aactagcata actaaaataa acaatgaaac ttatggctat
    26161 cgctataaat ttctaaaaat ataaactata aaaaactata aaattaaatt tttggaggat
    26221 aagattatgg caagtgcaaa cgcattagga atgatagaaa ctaaaggatt agtaggagca
    26281 atagaagcag cagatgcaat ggttaaagct gcaaatgtac aattagtagg aaaagagcaa
    26341 gtaggtggag gattagttac tgtaatggta agaggagatg ttggagctgt taaagctgct
    26401 acagatgcag gagctacagc tgccgagaga gttggagaac taatatcagt tcatgttata
    26461 ccaagaccac actttgaagt agatgctata ttaccaaagg tatcagctga gtaatataag
    26521 aataattata aaatttaaat tttgattgaa aaagaggcta tctcaaaaat gagtcttact
    26581 aataaactca atgtattttg aggtagtctt ttttatacat ctaataggag atgtgataaa
    26641 atgggtgttt taacagaaaa cgaagtaaaa agaaaaattg gaaaaaacaa aaataatata
    26701 actgaatttt atatagaaaa aggtcaaata ataactcctt ctgcaaaatc ttatttagct
    26761 gagagaagta ttgagcttaa atatgttgat aaactttcag atgtaaacaa tgaacctaaa
    26821 gaagttgtaa aagaagtagt taaagagaaa aaatataaat atactactgt atttggcgcc
    26881 aaattggatg aaaaaccaga acatatgact caattaaatg gaaatctttt agtttttaaa
    26941 gaccataaaa gaataatatt gagaggaaaa atagattctc ttgaatcaga aatactaaag
    27001 gtacagatac tttgtcagaa actagatatg ccaaagcttg ttaaggaatt gcaagaagta
    27061 ttagactttg taagaaatat aatgagatgt gaagttttag atgaaaagct aggggacttt
    27121 aaacttttag gattaactcc tcaagaactt agagaacaat ctcactatcc taaaaaatac
    27181 tttggtatag gacatgagtt cccaagttat gaaatgggag aagtagtagt agctataaat
    27241 agtataagaa gtaatacacg agaggttgaa cttgcagcct atgaagcatt taagggtgag
    27301 tatggaaatg ttcacagaga agatttaata aaggcactta atagattatc gtcagtattt
    27361 tggataatga tatataaggt tagaactaat aagtataagt agttggagga cataatggag
    27421 aatctattaa atgaaatagt ggaagaagta ataactagag ttaaaaaaga agcatttata
    27481 gaagttgaag catcaggaag acacgttcac ttaagtagag aagatgtcga taaattattt
    27541 ggagaaggat atactcttac aaaactaaaa gacctatctc aaccaggtca atatgcatgt
    27601 aaggaaagag ttacaataac tggtcctaaa ggaagtataa aaaatgtagt tgtacttgga
    27661 ccatgtagaa atgaaactca ggtagaaata tctttaacag atggttcaac tcttggacta
    27721 aaagctccta taaaacaaag tggagactta gaaggtactt taagtattaa aatatcaaca
    27781 caatatggtg aagttgaact tgataagggg ctaatggttg ctaaaagaca tatacacatg
    27841 actcctaaag atgctgaaaa attcaatgta tgtgataaag agatagttga agctaaggtg
    27901 atgggtcaaa gacctttaac atttgatgat gtagtaataa gagtgagtga cagttttaaa
    27961 acttatatgc atatagacta tgatgaagcc aatgcatgtg gttattcaaa aggaactgtt
    28021 gctaaaataa taaagaaagc ataaatttaa cctactatat aaattacctc tagggaggat
    28081 ttaatatgaa ttatgataac cttgtagatt taataacgga agaaatctat aaaaaaataa
    28141 atagtaatga aataaaaata tctaataaac ctaaagctgt gattgtattt gaacaagata
    28201 acaataaatt taacttatta aaagatggat ttgaaatagt agaatttgat aaaagtataa
    28261 gggaatgtga aatagtgata gtttcaagac tttgtatgag ggggctatcg aatatagcac
    28321 ttggaaattc aacaagtgat gaagaaagat ttatactaaa aatgattatg aatggcaaaa
    28381 aagtatatgt cctagatgaa ggaattgaat acaaaaaata taaggatact gcaccaaaag
    28441 cactatataa gaaatttatg tcatttgaag atgaaatatg taaatttggt gtagaaatta
    28501 taaaagactt aaatgttata actaaaaata agttaaatat aaagaaagaa ctagaaagta
    28561 ctagaggttc taaaaaagaa gatacaaaag ttttagataa agatatttta aatttaacca
    28621 gtgtagaaag tgtaaaaata gataatgaat ttagtttgga tttaaggaat aaaaaactta
    28681 tatcggaagc tgacttaaga aaaccaacta taaatggagt taaaaacata ttagttaata
    28741 aaaaaagtat aataacacca ttagctgtag attttatgag aatacaccat ctcaaactaa
    28801 agaaactgta aggaggtaat tttatggata taggaaaagt tgtaggtaat gtttgggcta
    28861 caagaaaaga tgaaaaatta tgtggccaaa aacttttagt tgtaaaaatt ttaaaaacta
    28921 aagacacata caaggaaggc ttcatggttg cagccgataa tgcaggagca ggaaatggag
    28981 acttagttct tatatccaaa ggttcctcag ctagagagtc gattgaaaaa tctcatgttc
    29041 cagtagatgc aactatagtt ggagtagtcg attcgtttga ggtgtttgat gaataaaagt
    29101 ataggagcta tagagtttaa aagcattgct aaaggtatag aagtatccaa tgaaatgata
    29161 aagaaatctt cagttgatgt attgtactta aaaagtattt gcccaggtaa atttctaata
    29221 atagtaggtg gagaaacttc ttatataaat gaatgtgtag attatggaat aaaacttgga
    29281 gaaggatata ttgtagataa cttcgtaata aatgcaattt cacaagaaat attagatgga
    29341 tttaaaaata agtatcaaaa gctagatagt atagtctcta taggcgttgt tgaaagtagt
    29401 aaaatatgcg ctggaataaa aatgcttgat aagactttaa aatctggcga cttagtatta
    29461 gtaaaacttc aattatcttt tgctataggt ggcaaattag tatatatagt agcaggagat
    29521 ttaagtagtt tagaatatgc attaaaagaa agtgaaaatg tagtaagaga aaaagaagta
    29581 atatataaga ctgtaatacc atcagtagac agtcaaataa taaaaagtct aataaaataa
    29641 tggggggaat tatgagtata aacgaaatta taatctatgt aatggtagtc ttcatggtac
    29701 ttggagctat agataaatgt ataggtaaca aatttggtct tggagaacaa tttgaggaag
    29761 gtataatggc aatggggtca cttgcagttg caatggttgg tgttatatgt ttagcaccag
    29821 ttcttgctga tgtattaaga ccaattgtag taccagtatt taatatactt ggagctgacc
    29881 cagctatgtt tgctggaagt ttattagcta atgatatggg tggtgcgcca cttgcacttg
    29941 aacttgcgaa agaccctaat gctgggttat ttggtggatt aatagtaggg gctatgatgg
    30001 gacctacaat agtatttata ataccagttg cgcttggaat aattgaaaaa gaagaccaaa
    30061 aattcttagc aacaggaata cttgcaggta taataacaat acctattgga gcattcgttg
    30121 gtggattagt tgcaggattc gaggtaatga tggttcttag aaacttagtg ccaataataa
    30181 tatttgcagt agttatagca cttggattat taaaattcga aaatgctatg ataaaaggat
    30241 tcacatactt tggaaaaggt gtagtaatag ttataacttt aggacttgct gctgccatag
    30301 ttgaagcttt aacaggaata gttgtaatac ctggaatgaa accaatcagt gaaggtatag
    30361 aaatagttgg agatatagca atagttttag caggagcatt cccattagtg tttgttataa
    30421 caaaggtatt taataaacca ttaatggctt taggaaaagt tcttggaatg aatgatgtat
    30481 cagcagcagg ccttgttgca agtttagcta actgtatacc aatgtttggt atgatgaaag
    30541 atatggacaa tagaggaaaa ataataaatg tagcattctc tgtatcagca gcattcgtat
    30601 ttggagacca cttaggattt acggcaggat ttaattctaa tatgataaca ccaatgatag
    30661 ttgctaaatt agttggtggt ataacagcag tattgttagc tatggttatc gcaaataaga
    30721 cactaaagaa ggagaatgta taagatggat atatcaaata tagataaaaa tttaattgaa
    30781 acattagtaa gacaaataat agaagaaaaa ataagtggaa ctaaagacac agtagatttt
    30841 gttagaaata aagacattag tggaataact tctataaagt taccaactgt aaaagttagt
    30901 gaatctgata gattagatac agggaatcca agtgatgtag tttacacaaa agatttattt
    30961 acattagagg aaagcccaag attaggttgt ggtatgatgg aaatgaaaga aacaactttt
    31021 gattggactc taaattatga tgaaatagat tatgtaatag atggaacttt agatataata
    31081 atagatggaa gaaaggtttc tgcttcttca ggtgaattaa tattcatacc aaaaggaagt
    31141 aagatacaat tttctgtacc tgattatgct agatttatat atgtaacata tccggcagat
    31201 tgggcatctc aaaattaata aaaatcaagt taagttaata atcaaataaa taatttaaaa
    31261 tacaaactct taaataagca cgcatattta aaaaagctat gaattaaaat tcatagcttt
    31321 ttttataaag aatgttatat tttagacgta ttttttctat atttactggg agatatatta
    31381 tattttttat taaataatct aataaaatgc tctacatttg gataaccaat actattttac
    31441 tgataatctt ttccttatca ctaatcttac attttaaaat attattttta ctcgtaatat
    31501 tatcataaga atttgtttct tatttaaaac taattagaat tataaagttt attgccatgt
    31561 tattaataaa ataaatccag ttaaatcgag tgctagttaa agtaataaga aacttttaca
    31621 atggtttcct ttatcgattg aaataaaagt taatatggta taatatctaa agagagaatc
    31681 ttatcaatta gaatatgcat agaaacaagt ggcttaaaag gagacgttat atggaagaga
    31741 tagtaaagaa ggaacaagca agtatgaatg atattttttt gtatcttaaa gctaaatcaa
    31801 actcattata taaatttgta atattatata atgagtttat caataaacct aaagattatg
    31861 gaactgggga acttataaac atggttgaag tacatatgct aacagcgatt gaagaaaatc
    31921 caggaataag tccaactcag ttagctaaaa tatggaacag aactaaaggt gcaatatctc
    31981 aaactatatc taaattggag aaaaagggtt atataactaa agaaaagaaa gaaggaaatg
    32041 caaaaactat attactatat ccaaccacat caggagtaga gttaagtaag gcacataaac
    32101 tatatgacac catagatgtt acaagaacat ttgaatactt aatcaaggag tgtacattag
    32161 aggagataga tagtttttat aaagtaattg atttgtttat taatttatta gaagaatctc
    32221 cgcaggaaaa taatgattct gaaaaagtgt agctgagatt gaagttgaat aaagagaaat
    32281 agatattata gaagtagtac ttaagtacac attatataaa aatataatag gtataggatt
    32341 aaaaagttta gtggtttaaa gtttaaccac taaacttttt taatgtctat aatctactat
    32401 tatttaattt actaaaaata aaaaaactca ttatattaag atagaaatag aaaaataact
    32461 agaaaaatac tataatatat cagcttaata cagaaaaaag ttaaaaaatt tgagaaaaat
    32521 aaaaatgaaa acgttttata ttgacataga gaaaaagtag tgataccatt taaatatagt
    32581 ttagtagcta aactatattt aaattataat aactatttta gggggatgtt atgaatttaa
    32641 aacaattaag tgaaaaatac gaagaatatg taattcaaat gagaagagaa tttcataagt
    32701 atccagaatt atcttttaaa gaaataagaa caacccaaag aataagggaa gaattacaaa
    32761 aaataggggt cccttttgta tctattgaac caaatatcgt aatagctact atacaaggca
    32821 aaaatgaagg taagacaatt gcaattagag gagatataga tgcgcttcca atgcaagaag
    32881 attcagatgt tgaatacaaa tcagaatatg atggaataat gcattcttgt ggacatgatg
    32941 ctcatggagc tatgttacta ggacttgcac atatgctaaa tgatataaga aaagatttaa
    33001 aaggtagagt attattagtt tttcaagcag ctgaagaagt tggtggtgga cacaaggaga
    33061 taatacagta ctttaaggaa aatggaggac cggatagatt aatagctact catgtttggt
    33121 ccgatattga agctggtaaa atttctgttg aaccaggacc tcgtatggct ggtgggtctg
    33181 gttggagaat agacataaca ggtagaggag gtcatggttc tagaccagat caaagcatag
    33241 atcctataaa gccagcttgt gatatagttt taaaagtatc ttctatacca gttaatcata
    33301 taagtgtact agaacaatgt gttgtacatg catgtgcaat acatggagga actacaatag
    33361 gaaatgttat tccaaacaat gcagaggtaa taggtggata tagatatttt actgaagaat
    33421 caggcaaaaa agtgtttgac attataaagc aaatgagtaa tggtgttgga caagtttatg
    33481 gagtggatgt gaaggttact catactggag gcataggacc tgttatcaat gatgaagaag
    33541 cagttaagat ggctaaagaa atagtttcta atatagatgg attggagtta gattcttatg
    33601 agccaatatg tgcatcagat tgttatggtg aaatattaaa agagtattct ggcttttatt
    33661 gttatctagg ggtaggtaac aaagaaaaag gaataatata tgcacaacac catcctaagt
    33721 acaatataga tgaagatata ttaaagttag gttgcgaatt tatggctaaa tatgcagttg
    33781 aatttttaaa taaaaataat aactaaaata aatctaattt tataaatggg gggatgaaaa
    33841 tgcaaggtgc attaaaggaa aataagaaaa aaagtggtgg aggattttta ggatggattg
    33901 aaaaagttgg gaataaaatg ccacatcctc tagcattatt tacatatatt acactgatag
    33961 tagtagcatt atctgcaata gcaagctttt taggtttttc agcaacaagt cctgctacag
    34021 gaaagttaat aaaagttatg aacttaataa gtgcaaaagg tttagtacta tttatgcaag
    34081 agtttgttaa gaacttccaa aacttccctg tgcttggagt agttttagtt atggcagttg
    34141 ccacaggagt ttgtgaaaag acaggatttt tttcaactgc cataaaaatg agtttatcaa
    34201 aagttaaggg gaatgcggtt gtatttataa tagcttttat aggagtattt gctaatcaag
    34261 ctggtgatgc tgcttttgta ttagtaccaa ctcttgcagg agcaatattt tatggattaa
    34321 atagaaatcc attagctggt atattctgtg gatttgcatc agttggtggt ggatttgcaa
    34381 cagctgtaat tcctggggga tgggacgtta cgctaacgcc aataactgta tcagctgctc
    34441 aaactattca acctgcattt gatatgacat tattagctag ttattatgca ctatttgtat
    34501 ctgcgtttat agtagctaca gtatcaacaa tagtaacggt taaatttata gaacctaaat
    34561 taggtaagta tactggtaaa ccagaaggta tagatgataa tcaaggtttt gaggtcacaa
    34621 aagaacaagc aaaagcagca aagttagcag gttttacagt tttagcttat gtagcgttat
    34681 taattgcttt gtgtatacca gctaatagtt ttttaagaag tccagaaggt tctttaatat
    34741 ttggagcacc acttatgtct tgcttacagt tttttatagt aatactattc ttcttgccag
    34801 ggattgtgta tggaatgagg gttggtaaaa tcaagactgt aaaagactta aatgatatat
    34861 tatgtcaatc aatggcagca atggcaccgt ttatcgtact tgcaatagtt ataggtcaat
    34921 tcttaacgct attctcagtg tctaatttag cacaggtact agctataaaa ggtggagcat
    34981 tactcaactc attgccacta ccaccacaag ctataatact gctattctta gtactagtag
    35041 cgtttataaa tatatttgtt ataagcggaa gtacaaaatg gatgatattt ggaccagtat
    35101 ttgttccaat gttaatgcaa ttaaacatac atccagcatt tactcagttt gtatatagat
    35161 taggagattc tgtaaccaat catttatcac cattaaatgc attctttata atacttttag
    35221 caacagtcca aaaatacgat aaaaatgcag gtatgggaac tatattctca gctatgatac
    35281 cttacactat aggttacttt gtagtacttg cagttttagt agtagtttgg atgacaatag
    35341 gtataccagt aggaataggt ggacaagttt ggttataaaa catatttaat aattttggga
    35401 ggaaaaaaaa tggaaacaac tgaaataaga tacaattttg atgaagtaat agatagaagt
    35461 ggaacaaact gtcttaaata tgatcttaag agtcaattta tgccagaatc tccagaagat
    35521 gcactaccac tatgggtagc agatatggat tttgcatgtg cccctgagat aattaatgca
    35581 atgcatgaaa gaatagacag aaaaatattt ggatatagct ctcaacaaac tgatgattat
    35641 tatgaagctg tatgtggatg gtttaagaga agatttgatt ggaaaataga aaaagagaat
    35701 atattcttta gcccaggggt tgtgcctgcg ttaggatttt tcacacaaat attaacacaa
    35761 aagggtgatg gtatcataat agaaaagcct gtatactatc cttttgaaga taaaataact
    35821 aacaatgaaa gaaaagtagt taatagtcct ttaaagtttg aagatggtca ttattctatg
    35881 gattatgatg gatttgaaga attagctaaa gatgaaaata ataaggtgtt tatattatgt
    35941 agtcctcata atccagttgg tagagtatgg aaaaaagaag agcttagaaa aatagtagac
    36001 atatgtaaga aatatgattt gtggataata tctgatgaaa tacattgtga tattattcgt
    36061 aaaggtgaaa agcatatacc attagaagta gcatgtcctg attataaaga tagaataata
    36121 acttgtacag ctccaagtaa gagttttaac ttagctggta tgcaattatc aaatatagta
    36181 atcaataata aggaattaca agaaaaatgg atgcttgaaa cagattctaa attagctata
    36241 ttcctaccta atccattcgc aatagttgct actaaagttg cttataatga ttgtgaagat
    36301 tggatgaatc aagtaaatga gtatatagat ggtaatatta aatatgtaga agattttgtt
    36361 aatgagtata tgcctaaagt aaagataata aaatctcaag gaacttactt agtttggtta
    36421 gactttagag ggtatggatt agatgcaaaa cagcttgaat atgtaatgct taacaaggcg
    36481 aaggtattgc tagatgaagg atacatattc ggagaagaag gagcaggatt tgaaagaata
    36541 aatgttgctt cacctagaac aattatagaa gagtgtatga atagaataaa atgtgcattt
    36601 gaaggattat aatagtaata tctaaataat atatttgtgt attttaaaaa aagactatgc
    36661 taaaaaaata tttcaaagca tagtctttta aagttttatg atgagtttaa aaaatttcaa
    36721 aacgttacta ttcacttata tgtttatatt agaaataatt ccacaatata ctcattttct
    36781 aaagttgcac ttttatacac cttgcttcac caatatcatg tgtaaccaaa atagtagact
    36841 tattctcatt cttaataata ctataaacat catcacaaac taataatcta gtctgataat
    36901 caagagctga aaaaggttca tctaaaagta gaatatctgg atttacagaa agtgttctaa
    36961 taagcgcaac tctttgtctc atacctccag ataattcttt tggatacata cttctaaaat
    37021 cccataatcc ataagtcttt aaaaggcttt caactctatc taaagatttt ttatcttttt
    37081 tgccttgaat ttccaatcca atagttatat tatccattat atttctccat tcaagcaaat
    37141 tatctttttg aaacatatat ccaatatttc cagtaatctt tatatcacca ctagttggtt
    37201 ttaaaagatt agtaagaata tttaatattg tagatttacc acttccagaa ggaccaagca
    37261 aagttaatat ttctccttct ttcaagttaa aattgacatc ctttaatact tcaagctcgc
    37321 cttccttagt gtagaagttc atatttatat ttttaacttc tactatgttg tcattcatta
    37381 tgccaccccc tataactttt agctcgtata tattatgtta ttaacaatat gacctatttg
    37441 taccattttt cataaatata atataaatat tttgtttatt tacgtatttt cttagtaact
    37501 tgtacgtata aattatagat aaataaatta taaggtaggg gataaatttg agtaacataa
    37561 taacgaattt aatagagtca atatttacta atccaataac tatattgttt ttacttgcaa
    37621 taactgtgta ttctgttttt aaaataatag aaaataacag agtatattca tttgtaagtt
    37681 caaaattaga cgaaatcaat agagactata agacaagtga gttttataaa aaggtaaggg
    37741 atgactacta tacatatagt aaagaaaatc cttatgcaga cgtaaatata acttcattta
    37801 tagaggaagt tgtatctgat ttaaagcata ataatttgcc attgttagaa aaaattagaa
    37861 gtataaaaaa ttcatcttca ataagtatat tacttggggt tttaggtacg tttgttggtc
    37921 tttctacaat gcttttatgt gttgatacta aagacattat aaattcactt ccttcaacta
    37981 ttagtagtat gcaaactgca tttacaacta gtatatttgg agttgtattt tctctaatta
    38041 taggttattt tactaagata aaagattgtg aacatgtttt gatacaaata atgttaaaat
    38101 ctgaaaattt actaacatct gaaataactc attttaagtc agaaagaatg gatttaaaag
    38161 tagaagaggt aaaaaataca ataaaacaaa taagtaagtc tatagaagct attgaaaaat
    38221 ttgataaaat atctaaagat ttaaatgact ttaatgatga atttatcagt ggtatagaag
    38281 ctttaaaaag ccttttagaa ggttctcaaa gttctattaa gacatttgac cagagcgtaa
    38341 gaaaattaga taaacagttt aatattttaa atataaaatt tgtaaagctt tttgataaat
    38401 atgataatca agataatata aataaagaga ttttatttga tataaaagaa tcttcaaaaa
    38461 atatatacaa tgccactgaa agccaattca aaattagaga ttatataaaa aatataaatg
    38521 caggatttgc tctatatgaa agaagtgctc aagatttatt aactaaatta atgacccatg
    38581 aaaataaaat ctctcaaaat caaaaaatcc ttttagatga aaagtttacc ttagatgact
    38641 caataaaaaa tctctcaagt attattgaaa acttttctaa tgacctacaa gttaaactgg
    38701 atatgatgtt tgaaaattct ttagatattc aagataagtt agatgttatg tttaataact
    38761 cttttatgaa tgatgaagtt cctcttgata gtgaagaatt atttaaggat gatgttaata
    38821 atgtatttaa tccttttagt gaagaaattt atgagataga ggataaagaa ataaaggtca
    38881 taggtgagga tgagttaaat gaataataaa tatagaagaa ctgttaacag agaatttgaa
    38941 aagagtagct tttggccaac atttacagat ttattatcta ctgtattgat ggttgtaatt
    39001 cttatcttat ttagctcaga aagtatttca ggttctgttg aacaagattt agctaagaat
    39061 gtcaatgctt ctgttgaaga aacttttaaa aaaagtggaa tacctgtgaa ggtagataaa
    39121 tctaatgggc aagtaacttt tggtgaaaaa accatgtttg atgtagatag tgatgtttta
    39181 aaaccagagg ctaaagaaat gctaaaaatg tttgttccaa agtacataga gactatttat
    39241 aaagattatg gtgactatat atccaaaata gttatagaag gtcatacaga tgatgtggga
    39301 agttatatat acaacttaga tttatcgcaa agaagagcat atagcgttgc taaatttata
    39361 gtgggagatg aaataggtga ctataaatac aaagataaag tgactaagca tattattgca
    39421 ataggaaggt caaaagctga actcatcaaa aatggtgaca atagcgtgaa tagagatgca
    39481 tctagaagag tagaactaaa atacgaaata aatattaatc aaaataaata attaaatttt
    39541 tataaaaaaa aagaaggcag ttggtgtgag ctttaagcct tctttaagga aaaagtttat
    39601 ttatttttgt tgatacttaa catattatag tttttagaag cagtttgcaa tatattaaac
    39661 gtaaatggtc gtttaaaatg cataactgga tactactgat gtaaaatatt tcattttata
    39721 taatataaaa attatacagt atcaattttc tatattattg tactataatc tattatttaa
    39781 aatattatgt aaaaaaatat cataaaaacg catatcatgt tataattact agagtattat
    39841 gaatatggag gcgtagtttg tgatagaaat aagtataaaa aataaaaaaa attctaaaat
    39901 taaaactttg atacttttat tgatagtatc tatatcaatt tatttaactg gttgtactac
    39961 attagataat ggaaataatc aatcaaataa taatgattct ttgtctagta gtatatcaag
    40021 tgataagaat gtaaaaattc attttattga cactggaaat agtgatgcaa tattgattca
    40081 agatggtaaa acttttatat tgatagatgg gggagataat gatgatgagg gattaatggt
    40141 agattaccta aacaatcaag gtgtaaaaga tattaaatat cttatagcta cccattctca
    40201 tgcagaccat ttaggtggac ttgattctgt tgtcaaaaac tttaatatag aaaatgtttt
    40261 tgtatcgaat ggttcagcag aaacaaagag ttatagggac tttataaatg ctttagcaaa
    40321 taagggttta tccccaagtg taccgcttga aaataataag ttctatttag aagattctta
    40381 ttttgaagtt ctaaatacaa atgggggaga tactactaat gaacaatctc tagtccttgt
    40441 atatactaat gggaatgata aagttttatt tacaggtgat gctgaagaag gtaccgaaaa
    40501 agaaatactc cctaagcttg agaaagtaga ccttttaaaa gtagctcatc atggttctcg
    40561 tagtagttca tctcaagagt ttttagataa agtaaatcct gaatatgcgg ttcttttagt
    40621 aggcaagggt aattcatatg gtcatccaca tcaggagaca atgaataaat taaaaaaaat
    40681 gggagtaaaa gtacatagaa gtgatgagtg ttcagatata atatttgaat caacaggtga
    40741 tggagtattt acaagttgta aagatggaag ttataataaa ggcgttagag aagatggaaa
    40801 ctctggaaaa tcaaacagta atacaagtaa aaatgattct tttaaaaata aacaaaatac
    40861 atccaattca gaagaaatag tgtattttac tccaaaaggt aagagttatc actctacaaa
    40921 aaattgtagt gggctttcaa gaagtaaaaa aatattatca ggcacaattg cagaaagtaa
    40981 gaaaaatgac ccatgtgata tatgttatgg aaaataacaa aaaaacaacc aatttatcta
    41041 taaagcttct gaactttaaa tcatttaaaa aataaaataa atgcataaaa aaataagcta
    41101 taatcaatag ataacacatt ttatctatac gaatagctta tttttttaag tattttttaa
    41161 tagctattta ataactctaa atacaccagt tacaatacct acaatttcaa cttctgagtc
    41221 attgagcata attggttcca taaaatcatt ttctggctga agtcttatta aatttccttc
    41281 tttaaaaaac ctcttaactg tagcagattc accatttata agtgcaacta ctatctgaga
    41341 atttaaggct gtattttttt tgtctactac aacgtaatct tcatctaaaa ttcctgcatt
    41401 aatcatactt tcgcctttta ctcttaatac aaaattgtta ctgcccttta ctaaactggc
    41461 agggaatgga atgtattcct ctatgttttg ttgagctaaa atgggttctc cagctgttat
    41521 ttgacctact aaaggaagct ctattatttc ttgattagct ccagaaacat catttacttt
    41581 actacgttct aaaacttcaa tagctcttgg tttagtaggg tcctttctta tgtatcccag
    41641 tttttcaagt ttatttagat gagagtgcac agttgaagta gatcttaacc ctacagcagt
    41701 acatatttct cttacagcag gtggataacc ttttaatatg atttgagact ttataaattc
    41761 caatatcaag acttgctttt cagttagatc taaatacata acatcactca tttccaaaca
    41821 tattttgttt ttatttccat acactttata tttttaatta taacataatc catttatatc
    41881 tgcaaacatt tgttcctgat ttttaatcgt cctcaatcca ttcaaaattc ttgactatat
    41941 cacgttttac atttttctta tgagctgcat aaagctgagt agttgtgaca ttatcatggt
    42001 caagtaagtt ttgaacatca tatatagaaa atcctgtttg gattagagaa gttgcagccg
    42061 ttgctcttaa tttatgggga ctatatccat tgtctctagt agtatcaagt ggaatagatg
    42121 tatatttttt taccagtgtt cttatagcct ttgctgtaat tctcttattt tgaagagaca
    42181 aaaataaagc atctttttct tcatcattta atagttcatc tcttgttctt tcgttttgca
    42241 aataatcttt tattacatgt tcacaagtat gatttatagg cattaaaact tctttcccac
    42301 gttttctata tattttaaat tcacctcttg aaaaattaaa agaggagata ttgagttctc
    42361 taagctcatt taatctaagc ccgtatgtaa caaataatgc tagtatagct ttgtcacgta
    42421 atttagtttt tctccaatat accttttctt tctctgtaag cccttcacct gtttctactg
    42481 attcaagcat cttagcaact tcatctatct caaggcgctt aatagcatct ggctgaggtt
    42541 taggaagttt tattggattg aatccatcag ttatattatt atctatctga gagtttctgt
    42601 ataagaattt aaatagagta gagatggatg atttttttct tgctaaagct ctattgttat
    42661 tttcaaaaat caatgtgttt ttttctgtat ttttgtaata tctactgcaa taatcaccta
    42721 agaataaatt tatatctctt gattttattg tattaaaatc atcttctgta atatctttta
    42781 cacaatctgc atttgttaat tctttggttt caatcaaata tgaacaaaaa aaattaatat
    42841 cttctaaata agcaagtcgt gtagaaactg caacagaacc ttttaaatat ataaaataat
    42901 ccttcataaa tacaggaagt ttattttcca aaagtagaca tttttctatg agttcatttt
    42961 ctttgataac aatattatca ggtttatctg atttattatg tacttttata attttcttat
    43021 cattattcaa aattaaacct cctaaaacta aaatataaat cttttatatt attttttgat
    43081 atttatacca agtgagtata aatatttaat ttaatcattt tttacatttt taagagatta
    43141 gcagtgcttt agatgaaatt atcactaact attccctaaa tccacattta aagactagtt
    43201 gaatttaagt actctttttt atttctcaat atatttatag aaatatttta aaatatattg
    43261 agaaattttt tatgctctac taaaatcttt tttatataat atttttatat gtttctaacg
    43321 aaaccaaata ttagtaaaat atgtatttat ttaactaaag tttttaaatg ggctgactaa
    43381 gcttaaaata taggatatta tgagagttac tattgaaaaa ataaacccta ttgccataaa
    43441 gaaacttata aatatgttat acttaactat taaactggat atattcaagt tatcaaatcc
    43501 aaataagtat gtaattgaac ttattacaaa aataactata actataaagc tcagaccatt
    43561 taagctattt tttaaatcag cactactaag tgacatatgt gtagatatag aatacattaa
    43621 aaacataaat ataaaatact tcaatgaata tacactaggt gttaacaaca ttattcttat
    43681 aaaattggaa atatcaccta aaataatgtg tataaaccca tttatattta aattattaat
    43741 catacttaca tataacttta aatctatatt ttgagtcact ttcatataac tatctggcag
    43801 taatatctta aaaattaaaa taatcattat tgttccacct ataataggtg caattcctat
    43861 aaagaaattt cctatacttt gataaagatt atctttttta tagctatgac ttacatatcc
    43921 tagtatacca tcatttttac tttctattgg tctgaaaaac ttaactgaat ttattttatg
    43981 catgaaaatt aaacacatta tcaaatgact caattcatga acaaacgttc ctatagctcc
    44041 agttacaata attccagttt ttccaaaaga tgaaaatata aatttattat ttagattttc
    44101 tgtaatgcta aaaatcaatc caaatacaat aaatattcct atgaaacaaa aaatatttat
    44161 tacacttatt tttaaaattt cgattatata aatcataagt taagtcctca tgtaaaagta
    44221 tttaactact ctacttcata tccattacag cttaaaattt tacaagcatt ttctatatct
    44281 ttttctttta ctaagatata gtctgtatca taagtagaaa ttgcaaatat gcttattttg
    44341 ttgtctgcta acaaactact taacttagat aaaattccta caagtgaaaa gtctaaagga
    44401 cctagtatct taagtattct ccactccttt tctgacttga catcagaagg aatattatta
    44461 tttaaacata caacacttaa ttcatcatca gttctagtta tagagtaaaa cttctctgta
    44521 tctatccatg ttggtatttt actgtcatta tttaatctac atactgcata ctcttctacc
    44581 aataatttta atttcataat gtatatttcc tccaatctta tttgtttact taagattata
    44641 cattaaaagt tacataaaat aaaacatata aagtaaaaaa ggttcactat agtgtcctat
    44701 ttctatagcc aacctttaaa acttaattaa attctaataa agagatatat tatctaggtt
    44761 caattataag tttaatggct gttttcttat caccttcgat ttctatatca gtaaatgcgg
    44821 gaatgcaaac taaatccatt ccactaggtg caacaaaacc tcttgctatt gcaattgatt
    44881 ttatcgcttg atttagagct ccagctccta tagcttgtat ttctgcaacg cctcgttctc
    44941 ttagtactcc agccaaggct ccagctacag aattaggatt agattttgat gaaactttta
    45001 gtacctccat ttcaacatct ccttgttaat ttgttaaagt atccattacc taaagtacta
    45061 aatcataatt gccatttact aattacaatc atcatttaaa gtatactttt ataaaaatat
    45121 acttctataa tcgttttaag aaaaccttta ttttttttaa ccattttatt atgaaaaaac
    45181 tacaaatttc tttatattct ataagcattt tccaatattt ctaaattttt tatatctttg
    45241 agctataatt tcctctttat ccatatcttt gtaatgattt aatctttcta aaatataagc
    45301 ttttatttct tttgaaacag cttctacatc tttttgtgct cctccaagag gttcttttat
    45361 aatcttatct attattttta aatcatataa atcattactt gtaagcttca taacatcaca
    45421 agcttcttta gctttagatg aatctttaaa tagaatacta gaaagtcctt ctggagaaat
    45481 tacagaataa actgaatgtt ctaacataca tacatcattt cctactccga tacctaaagc
    45541 tcctccacta ccaccttcac caattacaaa agtgattact ggaacagaaa gtttactcat
    45601 ttcaagtaag tttcttgcaa tagcttctcc ttgtcctctt tcttctgctt ctagaccaca
    45661 aaaagctcct gatgtatcaa taaacgttat tataggtcta ttaaattttt cagcttgttt
    45721 cataagtctt aatgcttttc tgtatccttc tggatgaggc attccaaaat ttctttttat
    45781 attttctttt gtatctcttc ctttttggtg tccaactata gtaacattaa actttcctat
    45841 ctgaccaatt cctccaacca tagaagggtc atctttaaaa agcctatctc catgaagttc
    45901 taaaaaatta gaacaaatat gttctatgta ttccaatgtc gtaggtctct ttatatctct
    45961 acttaatgtt accttttcat atgctgatag atgtgaaaaa gcatcttcct taagcttttc
    46021 caatttctca ttaagtgaat ttattttatc acttaaatct atattatttg ccttagaaat
    46081 agttattaat tgtttaatat cattttctaa gacttttatt ttttcactat tattttcaat
    46141 caaaacagtt cctcctttat gccctatttc ttgtgcatag caagaatttg atataatact
    46201 tccttcattt ttcttctatc tactatcatg tcaataaaac ctttttcaag taaaaactca
    46261 gaagtttgaa atccttcagg aagtttttga tttattgttt gctctattac tcttggacct
    46321 gcaaaaccta tcaacgtatt tggctcagaa attataatat caccaagcat agcaaaacta
    46381 gcagttactc ctccagtagt agggtcagtt agaactgata tatatggcaa taatttttct
    46441 tcaagcttag aaagtgcttg agaagtcttt gccatctgca taagagatac cataccttct
    46501 tgcattctag ctcctcctga agcagaacaa ataattatag gtaaattatt ttcagcagca
    46561 tactctattg aataagttat tttttcccca acaatagaac ccatacttcc catcataaaa
    46621 tcaggattca ttacacaaat aactgtttta attccattta ttcttccata acctgtgaca
    46681 acagcttctg actcttttgt tttttcctta taagagtcta ctttttcttt gtatttagga
    46741 aaatttagtg gattttgaaa ttctacatca gaattaaact ccataaaagt tcctttatca
    46801 atcaataaat ttattctttt tcttgctctt aagccataat gtcttgaaca ttttggacat
    46861 acaaatgaat tttcttctat gtcctttcta aatacatgac tatcacaacc tttgcaatag
    46921 gtccaaaatt tatcatctac actattcttt ttaaagttgt catctaaaga aatcgtaaca
    46981 tacttagatt cttttgatga taaaaatttt tttatcataa tctatttact ccttataatg
    47041 tagcaaattc tttcttcata aagacatctt ctataaattt ggtatgatga gtatcttctc
    47101 taaactcctc acttgttaca agtgcctttt gaaattctac atttgtatta ataccttcta
    47161 caataatttc gtctagagct ctatatattc tacttataca ttcttctcta gtttcagccc
    47221 aacatattaa cttacctatc atagaatcat ataaaggtgg tatagtatat cctgcatata
    47281 caaaagtatc aaatctaaca ccaaaacctc caggtaaatt taatgattct atcttacctg
    47341 gtgatggagc aaaattcttt gatgaatctt ctgcatttat tctacattca atagcatgac
    47401 ctctaaaagt aatatcttct tgtttaaaag gaattttatc cccattagct atagatattt
    47461 gaagttttat aaggtcaaga tttgttacca tctctgtaac tggatgttca acttgtattc
    47521 ttgtattcat ctctataaaa taaaagtttt tatctttgtc aactataaac tcaatagtac
    47581 ctgcaccgat ataacctact ccttttacta tatctactgc aattttatga agtttttgtc
    47641 tttcttcatc tgaaaggtat ggacttaaag attcttctat tactttttga tttcgccttt
    47701 gcatagaaca atctctatca cctaaatgta ttgcatttga atgtttatct ccaaatactt
    47761 gtacttctat atgtctagga ttttctataa acttttccat atataaatca ctattagaaa
    47821 atgctgctag agcttctgaa cttgccatat caaaatttga aaaaagttct tcttcttttc
    47881 taacgattct catacctttt ccaccgcctc cacttgaggc ttttatcata acaggatatc
    47941 caatttctct tgcaacttct aaagcttctt ccttagtttt caaaacagat tctgaaccag
    48001 gaacaacagg gatattcaat tccatcatag tttctcttgc tctagattta tctcccatca
    48061 aaatcatttg ttctcttgtt ggacctataa aaataatatt attatcttca cattgctttg
    48121 caaattcagg attctctgat aaaaatccaa agcctggatg aatagcatca cactttaatt
    48181 taagagcaag atagattata ttttctatat tattataact cttacttata ttatttggtc
    48241 ctatacaaat agattcatca gctatatatc tatgaaaaca atctttatct atttcagaat
    48301 agatagcaac agtttttata cctagttctt tacatgttct gatgattcta actgctatat
    48361 cgcctctatt tgcaactagt atttttttta tcaacttaaa tacctcctac tacaatggtt
    48421 ttatcttaaa taaaggttga ttatattcta ctagttcttc attactaact aatacttcaa
    48481 ttatttcacc tttaatttca cttgttatct cattcataag cttcattgct tctaatatac
    48541 ataaagtgtc gccttcttca actacatcgc caactttaac aaagctatct gcatctggac
    48601 ttggagaatc atagaatgtt cccatcaaag gagctactac tactgataga ttctcattag
    48661 atgtgacagg tgtatcgttt acaactggct tttctaaaga aagatccact actgattctt
    48721 gttgtacact taaaacaggt gaacttgtag attgagcttt tttagatact tctaatctta
    48781 aatcgctact ttcaagtttt acatattcta agttagttga gtctatggcc tttaataatt
    48841 ccttaatttc attgatgttc attttgttac ccccttgtat tattacctgg tgctaatatt
    48901 atatcacaaa aaacaaattt ataaaagaaa aagtgctgac tagagaatca acacatcaat
    48961 atatctttta attttaaatt aacaaccagt aacttatatt tgttttaatt ttccattatt
    49021 aatataaatt ataagtataa aatgaataaa attcaatatt ttttgcaaaa tacaagagaa
    49081 tacaaattaa tacctcggga atttttaaga caaataccaa tattatgcaa tttagacttc
    49141 gctttctttc aaattagtat cattttcttt atttttacat aggatgtact ttaatttagc
    49201 tatcaaaaca ttgctatata ggttaaaagt tattagaatt gcaggaatta taaagtaaac
    49261 tctagcacct ataaaaccaa aaactactgt aataagtatt aatcttttat ttaaagtaaa
    49321 aaaaggtata ttaaaaatac ttttgtcgga tgtttctgga gtataagaat atataactcc
    49381 aaaaatcaaa gtaagcatac aaagtggaag tagtgtataa cttgaaatta ttcccagaaa
    49441 tcctatactt tcaaaatatt tgctagcact atttgtataa acaaacatgt atccatttaa
    49501 atatgcgcat atacatgata taatgccaaa agttccccct aaggattgag tgtaccaatt
    49561 aaaaaattta gattccatat acctcacctc ctaaaactgt atatataatg atatgcattt
    49621 aaatagtaaa atatatcttt atatgtttct gaataaaatt ttgttaattt cattaattta
    49681 tgtttaagaa atttgaagta tagtgtagta tatattaatg tagatgaagt tttatagtac
    49741 ttatttcata gtgcttaatc aagttatctt attatggatt caaaagttgt ttgtagggtt
    49801 ttatattttg ttgagctgaa aaatttatta taaatgaagg gatgttttcg ctttggtaga
    49861 tacattagaa tttggtctta aaattttatt ttttatatta tcaataattt ggatgggtaa
    49921 aataatgatt ttaagaactg ataagcaaat agtaatcaat cctttgctta ttggtatatc
    49981 tgcagtcctt gtgatgttgc atacctcaca gagtaacatt gaattttttg gattagatgt
    50041 ccaatatata agaatagttt tgtatataat atattcactg ataattttaa taggaatatg
    50101 ggctactaac agaagaaatg gaatttttta gataatctat ttctataaaa tttaaaggtt
    50161 gctagtaaca aagcactaat attagcgttc tgttactaac aacctttatt ttgattatac
    50221 acttaaattg aaaatagaat gattgaataa gttgacagat tattttacat tgacagtaat
    50281 tagaacttct tgagatgtat ttaaatcatt tttgtcgctt agtataactt ttaccactgc
    50341 ttgacctgtt ttattacctt taattatcat atcttcacat gaaagtacac tcttatcact
    50401 tacagataca ataaaattta aatcttttgg aaggttatca gtaacttccc agtatttata
    50461 ataactatca attttcttcg taattatttt tctcaaatca atactttcac cttttgctat
    50521 atcaacgtta tgatatttag agtccagttt atactgatta tcttttgtat caataaaatt
    50581 taaatcgcaa tttacatcca caccattatg agaaagagaa tttactacat cgtcatcaat
    50641 caaataatta ttggcaagat ttattgaatc catatctttt agacttaaag cttccttagg
    50701 aatctctttt atattattat tccaaaaaat aaaattgtct acattttcaa gatatgatat
    50761 accttttaaa tttgaaatgc tctcaccagt tgcataaaat tctccatcta aagcctttat
    50821 attgcttata gtcatctcat ctggatttat atatcttttt acaactgctc ttaaatttgc
    50881 atcaggaaaa atatctttaa gttgattttc gctcaaaacc ttatcatttg aatttattgg
    50941 acttaaagct gctgcagaaa taataaatgg tattaaatta gctaaattca tagtaccctc
    51001 ccacataaat tttttattat aataatttaa tttctatgag agggttctgt ttccttgtta
    51061 tatataccta ctttcgtaca ctttattaat ataaattaaa ataaatttct actattaaac
    51121 ttataaacat attactagtt acgtttttta gcttcacatc taaatattcg cataccttgt
    51181 gacataaatt tgtcttcata ctctgttgta acattatttt catactcact atttcctaaa
    51241 tcaagagaaa tattctttaa tagccaatta tttgcagcaa tttcatttaa actaaactcg
    51301 aataattttt cattatctgt tttaaagtga atttcgccat catcttccaa aaccctattg
    51361 tacatttcta aaaatccaga atgagtgagt cttcttttac tccatctatt tttaggccat
    51421 gggtcacaga aatttatata aattctcgat aattctttag cttcaaagta atccaaaata
    51481 ttgcttacat ctccccataa aaatagtata ttattcaaat tagaagcatc tgctttttca
    51541 acagccttta aaagaacttc ttccttaagt tccatggcaa tataatttat gtcagggttc
    51601 tgcttagcta aagttgttat aaacttacct tttccagtcc caaattcaac atgtattgga
    51661 ttatcatttc taaactttaa attccacttg ccttttaatt tgtctatatc gtctcttaaa
    51721 acatattttg tataacttaa aagcttttca tctgcacctt tttttcgtct tcttctcaca
    51781 ataattctcc ttacatataa aaaattagta aaatacattt ttattataca ttatctcacc
    51841 aaaaaataaa aagaactctc ttaaaataga aatttatttc tataaagaga gttctaaact
    51901 tactaaaaac ttaatcttag tggcaccctg aacaaccaga gcatcctcct ccaccttgat
    51961 ctttcatatt ttcaggaata actctaaatc caaatcctgt atcttctatt attatatctc
    52021 catactgact atattcatca gagctcatta caaattgtaa ttctccaact tcataagtta
    52081 agtcatcttc ttttttctca tctaaagcta atccaaaaga aggacctcca caaccaacac
    52141 ctgcaaagta tactcttata ttattaggtt tatcttgatt gtcttttaag atacttttta
    52201 aagtatcaat agctgtttct ggtaaagtaa ttttcatcaa atcattccct ttcaaatatt
    52261 ttattaaaac aatatctatt atatacccat acaaggtaca taatcaatta gtttttgcat
    52321 attttactaa aacttctacc taatattacc taaggcttta aaatatttta tataatattt
//
LOCUS       DAMJVI010000014        51531 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.014, whole genome shotgun sequence.
ACCESSION   DAMJVI010000014 DAMJVI010000000
VERSION     DAMJVI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51531
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.014"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            123..3669
                     /locus_tag="PC545_000939"
                     /pseudo
     CDS             123..3669
                     /locus_tag="PC545_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            3854..5788
                     /locus_tag="PC545_000940"
     CDS             3854..5788
                     /locus_tag="PC545_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDF2455729.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLESAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHREKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLSREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACKKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKNEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRSNKNQQKSSTKKKKKH"
     gene            5894..6889
                     /locus_tag="PC545_000941"
     CDS             5894..6889
                     /locus_tag="PC545_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2455730.1"
                     /translation="MKKYISIILLVVLTMVVLVGCSPGKDNPKNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKEGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     NYSDIKFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            6861..7646
                     /locus_tag="PC545_000942"
     CDS             6861..7646
                     /locus_tag="PC545_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2455731.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIMLNSSFYIDILYSMGRCFFSFLLAMIFAIILSIVSYLNRFFRN
                     LLKPINALTRSIPTMILVVLALIWFEKDSTPFIVGFTIVFPILYDNVLGAILNIDKNL
                     LEMANVYKIRFIDKVLKIYLPAIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            7634..8332
                     /locus_tag="PC545_000943"
     CDS             7634..8332
                     /locus_tag="PC545_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455732.1"
                     /translation="MEKIKLSIENINKRYDSRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNRVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTQLNKICDEYLELVGISDYKKFYPQMLSGGIRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFRNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSKELEKNEVLKLI"
     gene            8455..8928
                     /gene="ybaK"
                     /locus_tag="PC545_000944"
     CDS             8455..8928
                     /gene="ybaK"
                     /locus_tag="PC545_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDF2455733.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKLTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            9125..9964
                     /locus_tag="PC545_000945"
     CDS             9125..9964
                     /locus_tag="PC545_000945"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HDF2455734.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLSCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEIGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTAINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            9977..10525
                     /locus_tag="PC545_000946"
     CDS             9977..10525
                     /locus_tag="PC545_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HDF2455735.1"
                     /translation="MIKITTPVNEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            10591..11772
                     /locus_tag="PC545_000947"
     CDS             10591..11772
                     /locus_tag="PC545_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HDF2455736.1"
                     /translation="MSKDYAKLALEMHEINKGKVSVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAIINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALEASIENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPAPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            11940..12326
                     /locus_tag="PC545_000948"
     CDS             11940..12326
                     /locus_tag="PC545_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455737.1"
                     /translation="MEYKVNDQELNASMFIPFVNRIWPGDYDTDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEEFYEKNGCQRSLQSYIIKKKDSY"
     gene            12476..12739
                     /locus_tag="PC545_000949"
     CDS             12476..12739
                     /locus_tag="PC545_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2455738.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            13265..13948
                     /locus_tag="PC545_000950"
     CDS             13265..13948
                     /locus_tag="PC545_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455739.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGKFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            13959..15200
                     /locus_tag="PC545_000951"
     CDS             13959..15200
                     /locus_tag="PC545_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455740.1"
                     /translation="MKIFSNKDIKIFFLMLISILVMFIIVGQIVVISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVAHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFILYMYFFRQQSKIEEASYKIDLFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIILNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISVNGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSIFTISFLKLTNM"
     gene            15272..15955
                     /locus_tag="PC545_000952"
     CDS             15272..15955
                     /locus_tag="PC545_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455741.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            15952..18282
                     /locus_tag="PC545_000953"
     CDS             15952..18282
                     /locus_tag="PC545_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455742.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDNGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANIGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KPEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEAKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTPGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLILHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPIKKICELDIIDSINAQ"
     gene            complement(18463..19305)
                     /locus_tag="PC545_000954"
     CDS             complement(18463..19305)
                     /locus_tag="PC545_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2455743.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVAHLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            19600..20334
                     /locus_tag="PC545_000955"
     CDS             19600..20334
                     /locus_tag="PC545_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455744.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGRPIEYTISIFRTDKYQYEIVLSE"
     gene            20344..21477
                     /gene="nagA"
                     /locus_tag="PC545_000956"
     CDS             20344..21477
                     /gene="nagA"
                     /locus_tag="PC545_000956"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDF2455745.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYDQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GNYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLNNTNLKVNEAINLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            21576..22325
                     /gene="nagB"
                     /locus_tag="PC545_000957"
     CDS             21576..22325
                     /gene="nagB"
                     /locus_tag="PC545_000957"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2455746.1"
                     /translation="MRVLVCKDYDGMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYNTVYGDITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPKDYKLV"
     gene            22477..22749
                     /locus_tag="PC545_000958"
     CDS             22477..22749
                     /locus_tag="PC545_000958"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455747.1"
                     /translation="MLKIIMFSGILSSIIKLPIIGILPTGNIKKNTGISDSKKDGKQR
                     RSNLGISIYQLPSFLYSLINNSVPSNKPIPPEEIKLIKMYLLKSCI"
     gene            23409..24113
                     /locus_tag="PC545_000959"
     CDS             23409..24113
                     /locus_tag="PC545_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455748.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNSIDMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAISRDELLNKIWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            24115..25602
                     /locus_tag="PC545_000960"
     CDS             24115..25602
                     /locus_tag="PC545_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455749.1"
                     /translation="MKNGNDKNRIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTEIVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTREDDFLKNVDEMELIRECIQNEKLDLN
                     SDANTRISTRNKDYYISTVKITSIYGEQDKYLILFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSTVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKHASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEEMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKYNQKNIVVIIEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIVNKHGGRIYVENGKKGAKFTILFKL"
     gene            25807..27420
                     /locus_tag="PC545_000961"
     CDS             25807..27420
                     /locus_tag="PC545_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2455750.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGFCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFVKEGFEIMLENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     LPLFLIPIFSLFLSVSIFDVSKFFSLESLKFLSLISSVGILYINFIVFYLFKFIIDKT
                     KLSMKYELLEKEIIHKEELRLSNECYKIIVEQTDSVVFEWNIKENKSFVSQAWTEKFG
                     YNNACKNIFKEIKDKDLVHSEDKAIFEGFLESIKKKNMHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELRTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQTSFGSKDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(27783..28613)
                     /locus_tag="PC545_000962"
     CDS             complement(27783..28613)
                     /locus_tag="PC545_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2455751.1"
                     /translation="MSQVSKNYFTTGEFAKICGINKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLREVKMPLKEIKAYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPVTYKEQFLGDEEENFLYECI
                     NFMDNYELSDYGTIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKPKGLYVTA
                     YHKGSYETIYKAYEKLLNFFNQNNLRIGDFVYEEYLLYDISVRDSNEYLTQISAEVKM
                     "
     gene            28686..30413
                     /locus_tag="PC545_000963"
     CDS             28686..30413
                     /locus_tag="PC545_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455752.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGV
                     VKGDIDYIVKTGLMMMIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKAMTIIIV
                     VVMFLFMLSAFLVGKKLIKLFKMMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DRTAIDYSETAISINKIFAVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNGHKDTVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISI
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            30450..32312
                     /locus_tag="PC545_000964"
     CDS             30450..32312
                     /locus_tag="PC545_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455753.1"
                     /translation="MSEKRIDEQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLISAVFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRKAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPKGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHKELIEKKGFYEELYNSQFTSEYDEDVV"
     gene            32645..33334
                     /locus_tag="PC545_000965"
     CDS             32645..33334
                     /locus_tag="PC545_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HDF2455754.1"
                     /translation="MNKKLNYRFEMKHKITEADVLALKSRLYQIMKKDENASKDGKYL
                     IRSLYFDTPEDKALLDKLNGVAIREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSTNL
                     TKKEVINILNNDIEFLKESTNCLLREFYLKLKCERLEAKSIVDYNREAFIYPVGNVRV
                     TIDSDIKTSVNSVDLFNKDLPTVSVIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            33439..34125
                     /locus_tag="PC545_000966"
     CDS             33439..34125
                     /locus_tag="PC545_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HDF2455755.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKVFTKYQIKSKSVTPEKGIELVFEVRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            34142..36049
                     /locus_tag="PC545_000967"
     CDS             34142..36049
                     /locus_tag="PC545_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HDF2455756.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGVYSTSSNKSVDLYSDVYIEKYF
                     NRDKVMEVNIEIDESDLKDMNENAIKEEFKVAKVTVDGDTYGNVGIRTKGNSSLISVA
                     NSDSDRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLATPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGNVTGDLYKSDEGSSLQYKGDDPESYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLHDMIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDNNNSNNSDSNNNSNSENKRSGNQSDEKEVNAELTSSVVKA
                     NTDNETKNKTTNDSESKNNTDKDKSGNDNNQKLEGPMGKGGKSIPGVLEVAEDMSKTI
                     KSQLSGETSSTKQNSGDESSSGIKGSEKFDEDMSGMPEPPEGMDGKMPPGMGNMDKGD
                     MNGKNGNMNMDRNQDNPIEVGGFGNRGGGSVSKTTTYFKLILGGASMIIMSIMLVGVS
                     RVKRRRFIKSK"
     gene            36500..37189
                     /locus_tag="PC545_000968"
     CDS             36500..37189
                     /locus_tag="PC545_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455757.1"
                     /translation="MLQKVLWKFLKLVVGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADN
                     LFVGIIMLILGLVLLSVGMVFYMGSGLGSGPRDGLMVAIQKKTGKSLKLIRGTIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAAVQNIVK"
     gene            37625..38164
                     /locus_tag="PC545_000969"
     CDS             37625..38164
                     /locus_tag="PC545_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455758.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            38183..39226
                     /locus_tag="PC545_000970"
     CDS             38183..39226
                     /locus_tag="PC545_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455759.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKENN"
     gene            39228..40064
                     /locus_tag="PC545_000971"
     CDS             39228..40064
                     /locus_tag="PC545_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455760.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYMKVFGRSILLAGGATLICLIVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            40169..40843
                     /locus_tag="PC545_000972"
     CDS             40169..40843
                     /locus_tag="PC545_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455761.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGIK"
     gene            40843..41895
                     /locus_tag="PC545_000973"
     CDS             40843..41895
                     /locus_tag="PC545_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2455762.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPMDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITQNPVAYPSKEVLDKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            42125..42973
                     /locus_tag="PC545_000974"
     CDS             42125..42973
                     /locus_tag="PC545_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2455763.1"
                     /translation="MNRINKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSVVGLITSSIIIFMYTSYILYNNITHNMDVEFISYMWIIATPVSSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGENKTNKIVKYIGSNIIECTRNEDIIYSLQKDMIGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYKSSFGDSINFKNIVEE
                     ELQYDV"
     gene            42963..44627
                     /locus_tag="PC545_000975"
     CDS             42963..44627
                     /locus_tag="PC545_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2455764.1"
                     /translation="MMFKIFNKSLLKLVVVLSIVLTISNFSLSSYASEITSSTDKVLI
                     IYDSKKETAYNRDILNIMRTLLGRFSSDIELLKLSNYDGEINKNYYSHIFILGINENS
                     YNNDKNTKNLISSLNSYKGTICWLGYGIENLLEHKKYNLDYVGKTNNIVSVNYRGKSY
                     NLDEHYVFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKTFDKGRIFVRIEDVHAFREPKNLVEIADYLSSKNIPFTIALIPAYVNPK
                     NHKVITLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLNGLRVCIENGIYPLAFEAPHYAMESEGYKELKKYFSTYMGQHQNND
                     KKFSTNTYPYIIRDTEEFNIFIPENLGYIDPEDKFTFQNIKENLNKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDKDLDE
                     ITKSDTRFKSISNISKVLIFIVSISVLIFVIIFIYFKRIDKKKFLK"
     gene            44676..45932
                     /locus_tag="PC545_000976"
     CDS             44676..45932
                     /locus_tag="PC545_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2455765.1"
                     /translation="MRDLNVSEYLFVFSLFSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMISILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILEN
                     IKDRYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDKNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIENIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            45955..48036
                     /locus_tag="PC545_000977"
     CDS             45955..48036
                     /locus_tag="PC545_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDF2455766.1"
                     /translation="MKKFIISIISLVLFFSNISLIYKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEEVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKIKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKVENSINEYPYPYAEIENNHKLDTTIVVPDNMTRGETTAVFNLASAFGKITKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSIKEQTLLSSNCIIKQVDSPYNKN
                     KKMMVVIGSNEDDLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIILNLRYSDNLDFEKSLVTVSIN
                     DVIVGSKKLDRSHSNNDKLELKIPKDIDNKNYYQVKLTFNLSIKNSNCVTRESNNPWA
                     YVSNNSYLALSMKENETLSFENYPYPFVRDDEFNDLTVIMPDYSGSQAMTWMFRLGVT
                     LGANINSHNGNINVIRGKEFSDKYKDTNIVVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FISNDKISFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKSKKEVKDSSWNMSINKTTKVFLMISFITIIVVMIL
                     SMLYIKKYKRR"
     gene            48073..49158
                     /locus_tag="PC545_000978"
     CDS             48073..49158
                     /locus_tag="PC545_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2455767.1"
                     /translation="MRKYMNKFKLKRSILISLIILIFIFIVYFVSIELKDRSLVNNQY
                     KGKIKSANLSVDYEINQVMKDIDKLGLNTVNVPIIINVESINSDIMSIDNNSKEKAIK
                     LIKKLNKKGISTILEAYPWIENGKLYETDWNPINKKRFFYTWQNAILNELIIDVANPL
                     DVNVLNIGSNFVHLEEYQQNWGEIIDFVQSKFDGFVTYRTNWWYTKKDDLSSKLFYEQ
                     KLNNNFFDKLDFISIAAYFELSNKPVNTVDELISALHSSTVNNRGQNIKQEIYNLYKA
                     HRKPIFFGELGFSNRESASSQPWNHTPSKAVNGEEQARCFEAYKKVFENEDWINGFSV
                     FCVGKIDDEKNFYPSKESIKVIKSWYE"
     gene            49212..50336
                     /gene="wecB"
                     /locus_tag="PC545_000979"
     CDS             49212..50336
                     /gene="wecB"
                     /locus_tag="PC545_000979"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDF2455768.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRENIKSIVCVTAQHRE
                     MLDQVIETFNINVDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIILVHGDTTTT
                     LATSLTAFYNKTLVGHIEAGLRTYDKYSPFPEELNRQLTGIIADMHFAPTNLARKNLI
                     SEGKPNNNIFVTGNTAIDALKMTIKENYNHPIIDEIGNDRMILLTSHRRENLGKPMKN
                     IFRAIKRIVDDFEDVQIVYPIHLNPKIRTIADEIFGKFPEKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGMQEEAPSLGKPVLVLRDKTERTEGIEAKTLKLVGTNEDRIYNSVSDL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            50414..51388
                     /locus_tag="PC545_000980"
     CDS             50414..51388
                     /locus_tag="PC545_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2455769.1"
                     /translation="MARKLIKNLGKSKSVKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQSLENAGYSTAEDKKGNRIYAELLIDVIRSYNLQLIDNKVETK"
BASE COUNT        20534 a         5243 c         8911 g        16843 t
ORIGIN      
        1 ttctaataca ggaagaaggt aaatgtatat tgaaaaaatc atagaaatag aagtaaacaa
       61 tttaaataaa tacaaaggtt tgcaaaagaa gtaatataat tatatacaat gaggatgtga
      121 gtatgatgaa cagactaaag ccaagaattt taggtattag tatcttgttt attattataa
      181 ttattttaac atgtggagct gttttcaagt ttaaatttaa attagataat aaattaaaag
      241 aaaccacaca tacatatttg tctgagttta cagaacacaa tataattaat ctcaagacaa
      301 gacttagtgg tcaatttgac atgcttgaat ctattgctaa cttcatagga aacttgaata
      361 atataaatga tgaaatggtg attgatttaa tggattctga tgtaaagcga agttctttaa
      421 ttagaatgac tatctctaca ttagatgggg aatgttattc taatgatgga attacccata
      481 atgttcaaga tagagattat tttaaaaaag ctaaaaatgg agaaaagaat atatcagagc
      541 ctttaaaatc tattgttaat aataaagaaa taatagtttt atctacccca gtttataaaa
      601 gtgggaaaat agttggtgtt ttaagtggaa tttatgattc aaaaaagtta aatgaattat
      661 taggattatc tgcatttgat gaaaaagcag atgtttttgt tgcaaagaaa aatggagata
      721 taattgctca aaaaacaaat aaaaatagta agacgatatc atgtatattt cagctttttg
      781 acaaagataa ttgtgatgaa gttgattgtc taaaaactat aaaagaaaat atgaaaaatg
      841 ataagtctga gtattttaat tataacttag ataatgaaaa atttatgtta aactatcagc
      901 cacttggagt taatgattgg tatgtttttt ctgttgtgcc aaataaaata gtgtctattc
      961 agtcagagaa tatatcttat gatgtatata tttttgcatg aaatactact agttatatta
     1021 atttttataa tatatatagc atgtattata aataataaca ccaaaattat tataaatgaa
     1081 aaagaaaaat ttaaagtact cactgataat atactaggag gggttaaaac ctgtttaaaa
     1141 gatgaatata tgactatgac atatgtaagt caggggtttt tagagcttac aggttactct
     1201 tgtgaagatt taaataaaat atttaaaaat agatttaaag acatgattta taaagatgat
     1261 ttagacaagt ttaattgtat tattctaaac caattagaaa aacaagataa aattgaagtt
     1321 gaatatagaa ttgtaagaaa agatggaagt ataatttgga ttttggagag aagtaagtct
     1381 ataaatgata ataaaggaag tgagtgtatt cagggagttt taactgatat aacagaattt
     1441 aagaaaatac aagaagagtt agattcaaaa agatttgaaa tacaggctat caatgatagt
     1501 attgttggtg gaatagtgat aactgagata aatgatgatt ttgatagtat atatttaaat
     1561 gaaggttatt taaatatgat tggatataca cgcaaacaac tgaaagatga gctaaataat
     1621 aaattaatca aacttatttt taaagatgat agaacagaat taattaatag tataaacaaa
     1681 caactcaaat taagtgatac atttgcatct gaacaaagag ttgaaaaaag ggatggcact
     1741 attatatgga ttttattaaa aggaagaaag atgatttatg ataatggaaa atctgtactt
     1801 tgttgtgtta ttgtagatat tacttctgct aaattattag agcaagaact taaaaagagt
     1861 gaagaacgtc atgaaatcat tctaaagcag acgcaaaata ttataataga atgggatata
     1921 gaaaaagata aaatgaatat atcacctaat tggtataata agtttaaata tgaccctata
     1981 actgaaaacg caacaaaaaa tataattaaa tcagtagata tatatcatga tgatatggat
     2041 aagtttattt cattaattaa acttacttca caaggtagtg agtatgaaga gtgtgaaatt
     2101 agacttagaa ctagagatac tatttacgct tggtttagta taaaggcaac aactttattt
     2161 gataataatg gtaaacctta taaagtagta ggaagtttga ctgatattga tgaatcaaaa
     2221 cgagaagctg aaaagttgaa agaaagggca gaaaaagacc ctcttacaga tttgtataat
     2281 aaaaaagcat cacaatcact tatagaagag tacataacat tggataaaaa aagaaaaggt
     2341 gctttaatga ttattgatat tgatgacttt aaaggaataa atgataattt aggacattta
     2401 tatggggatg ctgtattaag tgaagttgca aatgacctaa taaatctatt tagagcaacc
     2461 gatatagtag gcagaattgg tggagatgag tttattgtat ttataaaaga tatttctaaa
     2521 acaagagata ttataaataa agcagaagaa ttggtaaaaa catttgaaag gtctttttta
     2581 ggtaaaggac gaaattataa aatatcatta agtgtaggaa ttgctaagtt tccacaagat
     2641 ggtatggact atatgacact atttagaaat gcggatatag ctctttactc agccaaaggg
     2701 aagggcaaga attgttatac attgtatgat aaaaaaattg ataaagtcca atatataact
     2761 aatacaagaa atgcaggaga tagtgataat gtagagatgt cttttaaaga acatatcaca
     2821 aattatatat ttgatatact atatgaatca agtgatatag aagtggcagt aaatttaatt
     2881 ctaagtattg taggtaaaca ctttaatgtg agtcgcatgt atatatttga aaattcagat
     2941 gataatcgag tatgtaaaaa tacatttgaa tggtgtaacg aaggtataac ctcggaaatt
     3001 aataatctta aaaatatttg ttacaaggat ttaggcaact atcttttaaa ttttaatgca
     3061 gatgggttat tttattgtaa tgatattgaa aatttaggta gtgatgtgaa aaaagtactt
     3121 aagccacaag gcatacgttc aatattacaa tgtaaaattt tagagggtaa tgaatttaga
     3181 gggttcatag gatttgatga atgttcatat aatcgtatgt ggactaatga tgaagtgaac
     3241 actttgacat ttatatctaa aacgctcaat atgtttttat taaaaatgcg tattcagagt
     3301 aagctagaag agtctttaac aattcaaaaa tctgtgatgg ataatatgga tatatgggca
     3361 tatgttgttg acaggaatac ctatgaatta ctatttatca ataaaaaaat gcttgagtca
     3421 gcaccaaaca ctcatgtagg tgatttatgt tataaagcga tatggaataa tcaagataaa
     3481 ccttgtaaaa aatgccctat gatatattta aaagatggtg atgatactta taaatcattc
     3541 atgcataata aaaagtttga ggtacaggca aatataacta caactacttt gaaatggttt
     3601 gatggtaaag atgcatgttt aatatttgga agtgaaataa ctagtattga agctgaagaa
     3661 aatacttaaa atactgtgtt atatatatac ttaaaatcgc aatttgaatt tattagactt
     3721 aaaatgtcta tataaatcat ttaagttgtg gttttaagta ttttttatga tataattaag
     3781 cgttaagaac aaaattaaaa gatacttttg tataaaaaaa tatttgttaa atatatattg
     3841 agcaggtgat aatatggaaa ataagttttt accaataagc aaacaagata tgatagatag
     3901 aggatgggaa gaattagatt ttgttctagt tacaggagat gcttatgtgg accatcatag
     3961 ttttggaaca gcaataatat ctagagtttt agaaagtgct gggtataaag ttggaataat
     4021 agctcaacca gactggagaa ctacagatga ttttatgaag ctaggaaaac ctagacttgc
     4081 atttctagtt aatgcaggaa atatggattc aatggtcaat cattattctg taagtaaaaa
     4141 gcatagagaa aaggatatgt attctccagg tgggaaaatg ggttatagac cagatagagc
     4201 tactatagta tattgcaata aaataagaga agcatacagt gatgttgcaa tagtaatagg
     4261 tggaattgaa gcgagcttaa gaagatttgc acactatgat tattggagtg ataaggttag
     4321 aaagtctatg ttaatagata gtggagcaga tttattagtt tatggtatga gtgaaaagca
     4381 gattgtagaa gtagccaatg cacttaatga tggatatgac ccaaaataca ttagacatat
     4441 tgatgggaca tgctatattt cagatacatt ggaagaaata tatgataaat atatattaat
     4501 tccatcatat aaagaaattt gtgaagataa aatgaagtat gtagaagctt ttaaaattca
     4561 atatgatgag caagacccat ttagaggtaa tataattgtt caacctcatg gaagtaagta
     4621 tctggttcaa aataagccag aaaaaccact tagtagagaa gaactagatg aagtatatgg
     4681 attaccatat cagaagacat accatcctgt gtatgaaaaa tttggaggaa taccagctat
     4741 agaagaagta aaatttagta tagtaagttc aagaggctgt tttggtagtt gttcattttg
     4801 tgcaataaca tttcatcaag gtagagcagt gcaaagtaga agtgaaaaat caattattga
     4861 tgaagcaata ggaataacaa atttagatga ttttaaaggg tatatacatg atgttggagg
     4921 tccaacagct aattttagaa gaccagcatg taaaaaacaa attactaaag gtgcttgtaa
     4981 aaatagacaa tgcttatctc catcaccatg taaaaattta gatgcagacc acagtgaata
     5041 cttacattta cttagagctg taagaaaatt acctaaaata aaaaaggtgt ttgtgcgttc
     5101 tggaattaga tacgattatg taatggcaga taagaacaat aaatttctta gagagttgat
     5161 agagcatcat gtaagtggtc agttaaaggt agccccagaa catatatcag aagaagtttt
     5221 aaagtatatg caaaaaccag caggaaaaac ttatgataaa tttagacaga aattttttgc
     5281 tataaatgaa gaattaggta aaaagcagta tttgataccc tatttaatgt ctagtcaccc
     5341 tggttctacc ttaaatagcg ctatagagtt agcagagtat ttaagagata ctcactacca
     5401 accagagcag gttcaggatt tttatccaac tccagggact ctatctacaa ccatgtttta
     5461 tacaggtatt gacccgctta caatgaagcc agtttatgtt cctaaatcaa aacgtgacaa
     5521 ggcaatgcaa agggctttat tacaatatag agcacctaga aattatgatt tggtatactc
     5581 tgcgttagtt gaagcaggaa gagaagactt aattggtttt ggacatagat gtcttataaa
     5641 gccaaaaaat gaaaagcctt attttaatag aaataattct aaaaaaaatg tatctaaagg
     5701 aacaaataaa aacaaaaaaa ctaatacaaa taacagaagt aataaaaatc aacaaaaaag
     5761 ttcaactaaa aaaaagaaaa aacattaaag tataaataaa aatacttaaa aaagaaggat
     5821 tattcgataa attaacagaa attagtataa ataaatataa ttattttaat gacgttacct
     5881 ttgggaggaa gagatgaaaa agtatatttc tataatatta ttagtggtgt tgactatggt
     5941 tgttttggta ggatgttcac caggaaaaga taaccctaaa aataaggagc tatcagttgt
     6001 caaacaagta aaagtagctg ttccagatgg actaccagca gtggcaatag ctaaacttgc
     6061 aaatgaaaat ccagagatta aagaaggata tgaaacagtg tatagcattg aaaagacacc
     6121 tgaagcgatt tccactagag tgatgaaaaa agatgcagat atagctatag ttccttcaaa
     6181 catggctgca atagcgtata ataaaacttc atcatataat attgttggaa ctgtaggaat
     6241 gggttcactt tatttggtgt caacagaaaa tataaaagat tactctgatt tagttggtaa
     6301 agaagttgga tgtactggaa aaggacttac tccagacata actataaaaa gtcttttgtt
     6361 acaaaaggat ataaattatt cagatataaa atttaattat gtaaattcag caagtgaact
     6421 ggtaccatta ttggctactg gtaaagttaa aacaggtatt gttccagaac ctgcacttag
     6481 tgctttgatg tctaaaaatc cagacataaa aattataaaa agtttaaatg actcatggaa
     6541 ggaagtaagt ggttcaaaag atggttatcc tcaatctact ttaattgtaa agtctgaatt
     6601 tttaagagat aataaagatt ttgttgattc ttttgtaggt caattatcaa atagtataga
     6661 ttgggcaaac aaaaatcctg aagaattagg tgcatattct gaaaaaataa aaatatctac
     6721 tgaatcaaag ataataggaa aatctcttga aagagctaat ttgaaatata ttccagttaa
     6781 ggacatgatt aaagattata aaaattacta tgagaaatta gctaattttg atgataagac
     6841 attaggaggc aaagtaccag atgaagcaat atactttgtt gagaaataaa aaaaataaag
     6901 aacaatttat agataaaatg gaggtaacac tttcttgtgt tatcctctta tttttatggc
     6961 aaattattgc tttaaaaatt gataatgata tttttttacc aacagtaggt caagtgttta
     7021 atagtataaa agagattatg ctaaattcta gtttctatat agatatactt tactctatgg
     7081 ggagatgttt ttttagtttt ttactggcta tgatttttgc tattatactt tcaatagtgt
     7141 cttatttaaa tagatttttt agaaacttat taaagccaat caatgctttg acacgttcaa
     7201 taccaactat gatattggta gtacttgcac ttatttggtt tgaaaaggat agtacccctt
     7261 ttatagttgg atttactata gtttttccta tattgtatga taacgttttg ggcgctatat
     7321 tgaatataga caagaactta ttagaaatgg ctaatgtgta taaaataagg tttatagata
     7381 aggtgttaaa aatttattta ccagccataa aatttcaaat tatatctata ctagtatcaa
     7441 cattctcttt aagtttgaaa gttgctatag caggagaggt atatagtcaa cctacatatg
     7501 gtattggaac tatgatacag actgaaaaaa ttaactttaa cacatctgga atatttgcat
     7561 ggataattat tgttctttta atatctgcta ttttacaaat agctcaaaag tttttagcaa
     7621 ggagagcttt tttatggaaa agataaagtt gagtattgaa aatataaata aaagatatga
     7681 tagtcgtatt atatttagag attttaatat tgatttttat gtaaatgagg taaattgtat
     7741 ccttggaaaa tctggttgtg gaaaaactac actcttgaac ataataagtg ggattattaa
     7801 aaatgacacc aacaatctaa atatcaaaga aaatttaaat agagtaggaa ataagcttga
     7861 agctagttac atatttcaag atgatagact tatagactgg ttaacagtag aagaaaatat
     7921 aaagatagtt gtaaataaat actataataa aactcaatta aataaaattt gtgatgaata
     7981 cttggaattg gttggaatta gtgattataa aaaattctat cctcaaatgc ttagtggagg
     8041 tattagacaa agagtaaata ttgctagagc ttttatatat ccatcaaaaa acattataat
     8101 ggatgaacct tttaaatcta ttgatgcaaa gaatacacag cttataatgg ataatttcag
     8161 aaatatatta agaaaagaaa agagaactgt gttatttgta actcataata ttgaggaagc
     8221 attgttttta gcagatagga tatttatttt aggagactct ccaattagaa taaaaaaaat
     8281 attaaaaaat tctaaggaat tggaaaaaaa tgaagttttg aagttgatat agagttataa
     8341 taatactgtc gttttaaaat atatttggtt aaaatctata agactatatt aaaatagtac
     8401 agaatattca aaactgtgta taatgttcgt attttataaa aaggaagtga atgtatggtt
     8461 aagactaatg ctatgagaat attagattca aataagattg attataaagt aatgtcttat
     8521 gaagtgaaaa gtgaacatgt ggatggtgtt gaggtggcac atgatatagg aagagatgta
     8581 aatgaagtgt ataagacatt agtaactcaa ggagttagta agaatatata tgtatatgta
     8641 ataccagtac atgaaaactt agacttaaaa aaggctgcaa aagtagctaa agagaaaagt
     8701 gtagagatga ttcatgtaaa agatataaat aagcttacag gttatattag aggcggatgt
     8761 tctcctatag gtatgaaaaa gctttataaa acttttgtaa atgaaagtgc aaagaattta
     8821 gatacaatta ttgtgagtgc aggaaaaata ggatatcaaa tagaactatc tccatttgat
     8881 ttacaaagac ttataaaagt agaatttgta gatgtaataa aaaaataaga attacatata
     8941 aaatatagag cttatattct caataatagg tgttgtaata ttaataaaat gaataataat
     9001 tttaaaataa atattcagtt acttatgtgt aattggtata aaaatattga gaaattttaa
     9061 aaacaatggt aaagtgtaag tataatcacg aatatttttt ataaagtaat ggagggatgt
     9121 tcgcttgaga aaaataaaat ctgaacaaat cgtcgagcag gtaaaaaaac tttgtataga
     9181 agcgagccta tatttaggag aagatgtttt aagttgtata aaagaaaaag cgaaaagcga
     9241 aaagagtgaa gttggaaaaa atatattgaa tatattagtt gaaaatgctg aaatagctaa
     9301 agaaaaaaat ataccaatat gccaagatac tggtatggct gtattttttg ttgaaatagg
     9361 acaagaagtt ttaattgaag gagacacact tactgatgct ataaatgagg gagtaagaca
     9421 agggtatgaa gaaggctact taagaaaatc tgttgtaagt ccaattaaca gagtaaatac
     9481 aaaagataat acacctgctg ttattcatta tgatatggta aaaggtgata aaattaaaat
     9541 agaatttgca gctaagggat ttggaagcga aaatatgagt aagatgaaaa tgttaaagcc
     9601 atcagatgga ttggaaggaa ttaaaaaatt cataatagat acagtttcag aagctggacc
     9661 taacccatgc cctcctatgg taattggagt tggtataggt gggactgtgg acaaatgtgc
     9721 tcaaatagct aaaaaggcac tttttagaga actaggtgaa tttaataagg acgaaaatat
     9781 agcaaaactt gaaagtgaac tattaacagc tattaataaa cttggtatag gacctcaagg
     9841 cttaggtggt actacaacag ctttgggatt aaacatagag acttttccta cacacatagc
     9901 aggtctacct gttgttgtaa atataaactg ccatgcatca agacataaga aggtagtaat
     9961 ttaggaggaa aataaaatga taaaaataac tacacctgta aatgaaattg atatagcaaa
    10021 attaaattgt ggagatacaa ttagtttgag tggtatactt tatacagcaa gagatgctgc
    10081 tcataaaaga ttgatagatt gtataaataa aggtgaagaa ttaccatttg acgtatatgg
    10141 tcaaggaata tattatgtag gacctactcc tacaaagcca ggagaagtta taggtgctgc
    10201 aggtccaacg acgagttata gaatggatga tttgactata cctcttttag aaagaggtct
    10261 tagattgatg ataggcaagg gaaaaagaag tgatgaagtt atagaaggta tgcaaaaata
    10321 cggagcagtt tatcttgctg caataggagg agctggagcg tatatttcaa attcaataaa
    10381 atcatgtgaa attattgcat atgaagattt aggagctgaa gctataagaa agattgtagt
    10441 agaagacctt aagcttacag tagcaataga tagctatggg aataatatat atgagcaagg
    10501 tcgagcaatt tatgaatgta aataatgttt agaaataaat atgaatatca aaattaaaaa
    10561 tagttgtgta tattttaagg aggaaaattg atgtctaagg attatgcaaa attggcatta
    10621 gaaatgcatg aaatcaacaa agggaaagta agtgtagaaa gcaaggtaga agttaaaact
    10681 aaggatgatt tatcaacagc atatacacca ggggttgcag aaccttgttt aaaaatacat
    10741 gaaaatcaag atgatgttta tagatacact tctaaaggga atctagtagc agtagttagt
    10801 gatggaagtg ctgtacttgg attaggtaac attggtgctg aagcatctat acctgttatg
    10861 gaaggtaaag ctatattgtt taaacaattt gcagatgtag acgcttttcc aatatgttta
    10921 aagactaatg atgtagatga gatagttaaa tctgtagagt tgatggaacc tgtatttgga
    10981 ggtataaatt tggaagacat atcttctcca agatgttttg agatagaaga gagattaaaa
    11041 aaatctttaa gtatacctgt ttttcatgat gaccaacatg ggacagcaat aatagttgca
    11101 gcagccataa taaactcaat aaagttaatt gaaaataaaa aaatagaaga tttagaaata
    11161 gttataaacg gagcaggagc tgctggtata gccatagcaa agatactact taatatgaat
    11221 gttaaaaata taatcttatg tgatagaagt ggagcattgg aagctagtat agaaaatcta
    11281 aactatgttc aaaaagaaat gttaaaagta acaaatataa gaaatgaaaa aggaccttta
    11341 aaagatatta taaaaggaaa agatgtattt attggtgttt caggtccagg agctgttaca
    11401 aaggaaatgg ttgaaagtat gtctgagaag ccaataatat tggcaatggc aaatcctgct
    11461 ccagagataa tgccagaaga agcaaaatta ggtggagcat ttataatggg aacaggtcgt
    11521 tcagacttcc ctaatcaagt caataatgta ttggcttttc ctggaatatt tagaggagca
    11581 ttagatgtta aggctaaaga gataaatgaa gaaatgaaga tagctgctgc atatgcaatt
    11641 gctaatacaa taagtgatga ggaaatatgc ccagagtata tattaccaga tgtatttaat
    11701 aagaatgtag taaaaaatgt tgctaaggca gttaaagaag cagctataaa aactaaagta
    11761 aatagaatat aaatttttat atgctcatat attcttactt ttatatacat atctttatga
    11821 ttaaacgtgt tagaaaacgt agtaaattta tactatcaga ctttaccttt attacttaaa
    11881 atgatataat atgaatatta taaattaata aatttaaatt tattgaggtg caagagataa
    11941 tggaatataa agttaatgac caagaactta atgcttcaat gtttatccca ttcgttaaca
    12001 ggatatggcc aggagattat gatacggata aaacacagac tgcattatca aaaactttga
    12061 atataactgc ttatgatgat aaagtgcttg taggatgtct gcgagttctt tcagatggtt
    12121 attattttgg aacaattaca gaattgcttg ttcttccaga gtatcagaaa caaggcattg
    12181 gtagtaagct tttccagctt gctaaagaga atacaccaac tatgctatat tttggtgcac
    12241 agccaggagt agaagagttc tatgaaaaaa atggatgcca aagaagtctt caatcttaca
    12301 taataaaaaa gaaagatagt tattaaagtt ccagctttca ataatcttaa aagtacaata
    12361 ttttagtgga atcatttcat tttttattac taatatgaca taaacatgtc atattagtat
    12421 tgatatactt ttaatatggt taagagccaa agaaaaatat aaggagtgat ttaaaatgga
    12481 agaaactaaa ttttgccaat gctgtgcaat gccaatggga caaactgatg aattatatgg
    12541 gacaaataaa gatggaagta aaagtactga ttattgtagt tattgtttta aagatggtga
    12601 gtttacagct gatataagta tggaagagat gatagaagtt tgtatccctc atatgcttca
    12661 aagtaataag gatatgactg aagatgaagc aagaacaatg atgaacaatt tctttccaac
    12721 attaaaacgc tggaaataat tttatgcaaa attagttata tgcaaaacat gaaatatggt
    12781 gttgagaata aggggtggtg tgctttatag cataccacct gttttgttac ttgctagttc
    12841 aggtattctt gtatgcttaa atatgttctc atctctcttt ctattttaaa ttgtaaaaat
    12901 ctaaaatatg ttatttatat tctacttctt tatccatgtc tttttctacc acaagtcaaa
    12961 tccaactaca aaatcatcat tttcaattca ctgtttaata gaattaacag ataaagagtt
    13021 ataaaatttt ctatttttta tataaaataa tacaggtcaa gctgacttct agctacttgg
    13081 gatagtgtat atatttatga tagttatagg agtaatacca atcattagac tgttgatttg
    13141 tgtagtcttt tgtaaaaaca tttgttttaa tatattgagt ttttgaaaaa gtaaaattat
    13201 aattataata aattaagtgt aataatgtat tagaagtaaa aataattaaa atagaggtga
    13261 ttaaatgagt agtatattgt tagttgaaga tgatttaagc ttgatatatg gattagagtt
    13321 ctctatacaa aaaagtggat ttagtataga catagcaagg acagtaaaag aagctatgca
    13381 aatgtatgag gaaaaaaatt atgacttatt gcttttagat gtatccctac ctgatggtaa
    13441 tggatttcag atttgcgaaa aagtacgaga ggtttctaat atacccatta tatttttgac
    13501 tgcttcagat gaagaggtaa atgtagtgat gggtcttgat atgggtggag acgattatat
    13561 aacaaaacct tttaaattaa atgaattaat ttcaagaata aaagcattac ttagacgtta
    13621 taatgttgca agtaatgtga atgaattaag ttcaaataat attactatta aattacttga
    13681 aaatcgtgtt tttaaaggta aatttgaggt ggaacttacc acagcagaat ataaattatt
    13741 gtgcttattt atgaaaaata aaaatattgt acttactaga aaaaacatat tggacaagtt
    13801 atgggatgga aatggtagct ttatagatga caatacatta tcagtatatg taagacgatt
    13861 gagaaataaa attgaagata atcctgaaaa tcctaagttt ctattgaccg ttagaagaat
    13921 gggttataag tggaatgtaa ttaagtgagg gtattaatat gaagatattt tctaataaag
    13981 atataaaaat atttttttta atgcttatat ctattttagt aatgtttatt atagtagggc
    14041 aaatagtggt tataagtata actaacaatt ataaaaaaac gttgatagag catgattata
    14101 atatagctgg atacttatat agtataggtg ttgataaatc taaaatacca tcagcatttg
    14161 ttgcacatga agataaatat attttaaaag gaaaagaaat actagataca gctggatatg
    14221 gatttgatat agatgttagc tttacaccaa gtgtaaaatt gtttcaaagt aaatatataa
    14281 aaactatgtc tatctattta ttagtatttt ttactatgat tatttttata ctatatatgt
    14341 atttttttag gcaacaaagt aagatagaag aggctagtta taaaattgat ttatttatga
    14401 atggaaatat aaatgaaaga ctaaatacat atgaagaagg tagcttatca aaattattta
    14461 gttcaataga tgttatgtcg acatctttaa atacacatat attaaaagaa aaacagagca
    14521 gagaatttct taaaaataca atttcggata tatcgcatca attaaaaact ccacttacag
    14581 ctttaataat gtacaatcag atactacaag aagaaagtca taatagtgat gtcgttgaag
    14641 cttttgtaca aaaaagtcaa agtgaactag aacgcattga aacattgatt aagaatttat
    14701 taaagattac aaagatagat tcaaaaacaa taattctcaa taaaaataac gttaatctta
    14761 aaaatttaat tgtcaaaatt ttgtatagct ttgaaactag ggcagaaaaa gaaggaaagg
    14821 ttatttctgt aaatggttca gaaaatacat tgttgttctg cgattatgaa tggatgtcag
    14881 aggctttaag taatcttata aagaatgcat tagataatac aaaagagaat gataaaataa
    14941 cgatagagtg gtttgagacg actataacca ccattgtttc agttaaagat actggaaatg
    15001 gaatccatgt tgaggatatt catcatatat ttaaaaggtt ttatagaagt aagttttcaa
    15061 aagataatca aggtttagga ctgggattac cactagttaa atctattgtg gaacaacata
    15121 atggtactat ttctgtagaa agtaaactat cacaaggaag tatattcacc ataagttttt
    15181 taaagcttac taatatgtaa gataaaatac atcttgcagt aaggtatata gattattatt
    15241 atattaatca attagaaagg tgagagagaa aatggatatt cttgtggtta ataatttatg
    15301 taaaacatat ggaaatggat atacaaaggt agaggcattg aaagatgtat cctttacagt
    15361 ttcaaaagga gagtttgtga ctgtagttgg agaatctggt tcaggaaaga gtacactatt
    15421 aaatctctta ggaggtcttg atactccaac atcagggaga atatttattg atggtaaaga
    15481 aatatgttca atgaaagagg aaaaacagac tatttttaga cgtcgtaaca tagggtttat
    15541 atttcaggca tataacctta taccagaact aactgtggag caaaatataa tattcccatt
    15601 gttattagat tataaaaaac cagatactat ttacttagat gaagtattga ctattttagg
    15661 tcttaaagat aggaaaaatc atcttccaaa tcaattatct ggtggtcaac aacagagagt
    15721 tgcaattgga agggctttga ttacaaagcc aagtattata ctggcagatg agccaacggg
    15781 aaatcttgac agtaaaaata gtaatgatgt aatttcactt ttaaaagtga ctgcagaaaa
    15841 gtataatcaa accattatta tgataactca taatacgaat atagcttcta cagcagatag
    15901 aatactacag gtttctgatg gtgtgcttac agacctgggg gtgaatagag aatgagaagc
    15961 tacttagaat tagtaccaca atatgcaaaa gtacataaga aaaaaaatag aactacaatt
    16021 ttatgtatta ttattgcagt atgcttagtt actgctatct ttggacttgc agatatggct
    16081 atacaatgtc aaaagataca atttattaaa gatgatggaa attggcacat agtaattaga
    16141 aatattgatg aaaagtttat atcagaaata aatagtagag tagatatagc tcaccatggt
    16201 agggggcaag tcgttgacaa tggagagttt aaatcaaagg agattttgat acaaggttta
    16261 gatgaagaag tagcaaagca atttaactta gaaataataa ggggtcgtta tcctattggg
    16321 gagaatgaga ctttaataga tgaggatgca gttaaaagct ttaatctaaa aataggagat
    16381 tttatcaaaa taaaattagc agatggtaga aataaaaatt ataagatagt aggtgaatat
    16441 tcatcaatat caagcttaag agctacagat aaacatggat taatactttc aatattgggt
    16501 acacaagcta ttaaaaaatc agaaacgtac gattattatt atattacatt taaagaaaag
    16561 gttaatatta gaaaagccac ttctgatatt aaaaatacct tcaatttaag tgataaacaa
    16621 attggtaaaa atgaaagatt actttctatt ataggtcaag gtgactctga cttttctaaa
    16681 ccattgtatt taacagcagg attacttttt gtacttgttt tagtagcaag tattatcatg
    16741 atatataata gttttaacat gagtgtaatt gagagagtta aattttttgg tctgttacgt
    16801 tgtctagggt catcaaaaca tcaagttaaa aaatttgttg ttttagaaag tttaatttta
    16861 agtataaaag ctattccaat aggcttagta ttaggaagtc ttgtaactat tatatcttct
    16921 atatttctta agtatgttaa tgagttattc agctcaatgc caattttaaa agttagtttt
    16981 attggtattg catttggaat aattgtaggg tttttaactg taatattatc agctattatt
    17041 ccagcaaaaa aagcttcccg tgtatctcca cttagcgctg tacgtgggaa tctaatgtcc
    17101 aaagaaattt taaataacgg gaagacaagt aaagttgcaa atataggaag ttttaaaatt
    17161 gagacttcta tgggattaaa tcatgctttt tcaagaaaga aatcttttgt acttatgact
    17221 tcttcatttg ccattagtat tatactattt ttaggtttta gtgtgcttat agaccttatg
    17281 tatcaaggac ttaagcctat gaagccagaa gctcctcata tatctattgt tgaccctaat
    17341 tttaagtctg ttttaactaa ggaagatatt aatgaaataa gcaatatcaa aggagtaaag
    17401 aaagtataca gtagaatgat gacggatata gaagctacat ataatgggaa gaagacaaaa
    17461 tcacaattaa tttcttatga aaagtatcaa tttaattggg caaaaaaata tctattaaaa
    17521 ggagaagtta atgaaaataa actacagtca gaaaaccttg cattagtaga agaggggcat
    17581 ggatttgata taggagataa gatatatatt aaaaatggaa atgctgaaaa agagttagag
    17641 gtaggaggta tactttcctc aattccattt gaggctaaag ataaatatat tggaaatatt
    17701 ataacatctg aaaatacttt tacgaatata acaggtataa agggttatat aataatggat
    17761 attcaggttg ataaagatac tcctggagat ttaattgaaa atataagacg tgtagtagat
    17821 gataacttag aggtcagaga taaaagacaa agtaacttag aggttaaaaa ttcgttttat
    17881 actatggcaa catttgtata tggatttgtg tttattattg cgattataag catgtttaat
    17941 atcataaata gcatgaatat aagtgttaca agtagaaaga attactatgg tgttatgcgt
    18001 gcagttggca tatccaacag acaattaaga agaatggtaa tagtagaggc aggttcatat
    18061 gcagtgagtg gttgtttatt aggtagtata ctaggattaa tattacatag gtatatgttt
    18121 atatcgttag taagctcaat gtttcattta gagtggcaag taccttttaa tttactactt
    18181 atcactgtac taactatgat aataataact ttattatcag taagaaaacc aatcaaaaag
    18241 atttgtgaat tagatataat agactcaata aatgcacaat aaatataggt attgatttaa
    18301 attaaaaaaa cgaagtaaaa atgaaataga aaatattttt catttttact tcgtttttta
    18361 tattataaaa tttttgttat gttacaaaat ttctataaag atgatgtgaa tctaaacatt
    18421 atatgacaac gtgagcattt aagttaaaat attttaaata tactatttta gataagttcc
    18481 ctcttcatct tgataaatta atcctttttt cataagcact ccaagagctc gtttaaaatt
    18541 tttcttactt gaattaaata atatagaaat atctttaggg tcagatttat cattaaatct
    18601 catatatcca tcagaacctt ctaaataact taaaatctta ttttctaaag tatcaagttc
    18661 atcctttcta tttcctctag gagaaagacc aagttttcca tcttcatata tttttatgac
    18721 atgtaaattt tcaagaacat caccagtttt tagttcagta aagtactcat tatgaagtat
    18781 tacaccagca tatttattgt ctacacatat cattgcagaa ttattagttt ggaatccata
    18841 tacaactcca gttacaatat ctcctacatt gtaagtatgg tctgttaata agtatgaatc
    18901 tatatctgta gtagcagcaa ttcttttgct tttgtctaaa tatatataaa ataaatattt
    18961 ttcgtctcta aatagtggat aagtcttagc tttaaaagga actaatatgt ctttaggaag
    19021 tcccatatct ataaaagtac caatgtctgt attatcaact acttttaaat gtgctacatc
    19081 cccaacctta gctaaaggcg gtattaaagt tgcagcagtt ctgtcatcag aatctttaaa
    19141 tatgaaagca tttacttcat cacctatgtt tatttcattt tttccgatta atctattatg
    19201 aagaagtata tcgtcttttg tgttattagt ttgtccatcc aaaaaatatc caaattcagt
    19261 ttttcttttt actgtaagtt tattaaaatc acctatgttt atcaaattaa aatcatcctc
    19321 tcattttata tttataattt agccataata tacttatgta gtagtttgtc acagtatcta
    19381 tattagtatt agtgaaattt acaataaata atatagttat ttatgtattt ttcaattctc
    19441 tagcttctta tatagtataa actccaattt cttttttgtc tttaggtttt attaaaattt
    19501 ttacaaaata agttaaaaag tattgaaata aaatttcttt taaagtataa ttggtatata
    19561 ccaaaattgg tatataccat atcgaaaagt ggtgactata tgaaaatagt aaataaatct
    19621 agtaatatac cattgcatac tcaattaagt tcaataataa gagagatgat agaaactggt
    19681 gagcttaaag agggcgatgc aataatgcca gaaagagaac tttgcaacat acaaaatgta
    19741 agtcgtatga cagtaaataa aaccattgtt ggattagtta cagaaggact tctttatagg
    19801 gtacaaggta aagggacatt cgttgcaaag caaaaaaaga agtatcagtt ttcaaatgta
    19861 aaaggtttta ctgatgttat gaaagaaaag ggagtaaaca taaaaacgga tatattatcc
    19921 tttgagatgg aactaccaga tgatttagtt aaaagaaagc ttggaataag tgataatact
    19981 acaaatatat ataagattgt cagacttaga tatactgatg gagagccatt tggattagaa
    20041 atagtatatc tatcagaaga aatgtgtaag ggtctaacaa aagggatatt ggataattct
    20101 tcattatata gggttttaaa tgaaaaatat ggctataaga tacaaaaagc tgaacaggta
    20161 atggagccag taatactttc agatgaagaa agtaagcttc ttgaaacaga tgaaggtgct
    20221 ttagctctta aacttcatag aaattcctat aatagagaag gtagacctat agaatatacc
    20281 atatcaatat ttagaacaga taaatatcaa tatgaaatag tattgagtga gtaggtgaat
    20341 aatatgaaat gtttaattaa tggaaagata atattaaaaa atcaaatctt agaaaataaa
    20401 gtattagtat ttgatgaaaa aataattgat atagctgata gtgtaccaaa agattgtgaa
    20461 gttatagatg ctgatggaaa atatatatct ccagggctta ttgatattca tatacatgga
    20521 aacatgggaa aagatacaat ggattcgact gatgaatcta ttgaaacaat ttcaaaatca
    20581 attatgagac atggagtaac atcttttctt ccaactacaa tgactatgga taaagaacat
    20641 gtttatgatg cacttgaagt tattaaaaaa gcccaaaata gaaagcttga aggggcacaa
    20701 gtattaggag cacatttaga aggacctttt ataaatgaga attataaggg agcacaaaat
    20761 gaaaaattta taataaattc aaaatacgaa tttataaaag aatataaaga tgttataaaa
    20821 gttataactt atgctccaga aaaagatatt gattttgatt ttactagaga aataaaaaga
    20881 tgtacagata tagttttatc tattggtcat agtaatgcaa attatgacca agctaaagaa
    20941 gctataaatt taggagtaac taatgtaact cacatgttca atgcaatgac tggtttaaac
    21001 catagagacc caggcgttgt tggtgcagca cttacaacta atgtgtacag tgagttaata
    21061 gcagatacaa tccatataaa taaagattta tttcaattta tactaaataa taaaggaaaa
    21121 gaaagactga ttttaataac agattctata gaagctggtg gtcttgaaga tggcaattat
    21181 tctcttggag gtcagaaagt catagttaag ggtaatgaag ctagacttga aaatggagca
    21241 ttagctggaa gtgtgttgtc tttaaataaa atggtattta acttcttaaa taatacaaac
    21301 cttaaagtga atgaagcaat taatttagct tctttaaatc cagcaacatc tttgggaata
    21361 aatgacaaaa aaggaagctt agaaataggt aaggatgcag atatagctgt atttgatgag
    21421 aatttagatt gtaaaatgac actttgcttg ggggaagtag tatataaaaa tatatagtat
    21481 acaaaacaaa ccttaaagac agtaaaaaca atataaaaaa taataaatta aacttacatt
    21541 agtaaaagta gtagttaaaa tttaggagga ataagatgag agtattagtg tgtaaagatt
    21601 atgatggaat gagtaaaaaa gccgctgaaa tgatagcagc tcaaatagtt ttaaaaccaa
    21661 attcaatact gggactagct acaggaagta caccagtagg tatgtataga gatttagtaa
    21721 aaaaatacaa tgataatata gtagattttt cagatgtgat gagctttaat ttagatgaat
    21781 attataagtt accaatatct aatgaccaaa gctatgatta ttttatgaaa gagaatctat
    21841 ttaatcatgt aaacataaag ccagaaaata ctcatctacc aaatggaatg gctgatgata
    21901 ttgaaaaaga gtgtatgaac tatgaagctt ctatagatgc agctggtggt atagatgtac
    21961 aagtacttgg aataggtcgt aatgctcata taggttttaa tgaacctgat actaaatttg
    22021 caaaaagaac tcatgtagtt gaacttacag aatctacaat agaagctaat gcaagattct
    22081 ttaaatcaag agaagatgtt cctaaaaaag ctgtaagtat gggaataggt tcaatattga
    22141 agtctaagaa aatattgctt ttagcttcag gagaagaaaa agctgatgca gtatataata
    22201 ctgtttacgg agatataact ccagaagttc ctggttctat acttcaactt cataaagaca
    22261 ctatagttat agtagatgaa gcagcagcaa gtaaattgaa tccaaaagat tataaattag
    22321 tataagaata gaatgtagtt taaataaaaa atagctatca ttgaataatc aaatttattt
    22381 aatgatagct atttttattt aaattagtta ttattgttta aattacttgc ttattacttt
    22441 ttacgaaaac tcgttatcaa gaagtatcca aataagatgt taaaaattat tatgtttagt
    22501 gggattctat caagcataat aaaattacct ataattggga tattaccaac tggaaacata
    22561 aagaagaata caggcatcag tgatagtaaa aaagatggaa aacaaagaag aagtaatctt
    22621 ggaatatcaa tataccaact accatccttt ttgtattctc taatcaataa ttcagttcct
    22681 agtaataaac caataccacc agaggaaatt aaattaataa aaatgtatct tttaaagtct
    22741 tgcatataac cagtgtaaaa ttgagtatag aaatcataat ttaatttaac ggctaaaaaa
    22801 tacataagca atagaatgat taaaaataaa agatatctaa gagctttttt cattttaaat
    22861 tctcccttca gaaatacttt atttagtttt attttgttgg aatctgattt tatcatttag
    22921 tttaagaaat ccataatatt agtgaagaat gaaataagta ttatatatga ataaatattt
    22981 ttagaattaa ggtgaattgt agtgcttaat ataactataa tattaattgt ttgaatattc
    23041 aataaaaagt tcgaaaaata tcaattctga tataaactga ataaatacag ttgaatatta
    23101 aattattatt ttatcaaaaa tatgcaaagt actacacctt caaatatcac ctaaagtaat
    23161 taaaaggcaa ttaaagtgaa gtagttcaag tatttctaaa ataagtttaa taaattctat
    23221 aataatttat tttttgcctt aaaattgcct taaaatccaa acaattttaa aactaatgta
    23281 ctataaaaag ctggtataat ttaaatatag taaaaaataa gatttatctt atctattggg
    23341 atttaattat ttaatcaata tatttatacc atttaattta aataaaaagt ctattaagga
    23401 gtgagcttat gagtaagtta atttatatag cagatgatga agataatata agaaatttgg
    23461 taaagacatt cttaaaaaat gaaggtcatg atgttatgga ttttaaaacg ggtgatgaac
    23521 ttttagaaca atttaatata aaggagtgtg acttagtaat actagatata atgatgcctg
    23581 gctcaagtgg ttttgaagta tgtacaaaac ttcgtgagaa aagtacagtc ccaataatca
    23641 tgcttactgc aagagataca gatatagatt atattacagg gataacctta ggtagtgatg
    23701 attattttac aaaaccattt agcccaatgt ctttggttat gagagtaaaa tctatattta
    23761 gaaggataga gtttgaaaag aaacaaaatt atgataaata tagtaactcg atagatatgg
    23821 aattaaaatt tggtgatgta ataataaata aaaagaataa aatcgttaca agtaaaaatg
    23881 taaatataga ccttacacca aatgaatata acctattgac ttatttgttt gaaaatatag
    23941 atagagcaat ttccagagat gaattattaa acaaaatatg gggatacgat atagaagttg
    24001 agacaagggc tgctgatgat acagtgaaga gattacgtaa aaaaatttta gatacaaata
    24061 ttttaataga aactgtatgg gggtttggat ttaggttgaa ggagaaaagt taagatgaag
    24121 aatggaaatg acaagaatag aattggaaag attaaatcca aatttaaatt aaagttctca
    24181 aagaaaaata taagcataaa aactaggatt ttgttgagta tactttgttt gataattata
    24241 gtatttatga taatattagt atcttttaat gtatttcttg atacatatat aaaaacaact
    24301 gcgaatgaag aattgacaaa gtctacagaa atagtagaac atatggatag taattttaga
    24361 cctattaagc caccacaaga tgatggaaag cttcctccca aagtattatc taattttatg
    24421 agaaatgtac aagataaagt taggatggca gaaacacaat cagatgctga tgctatggta
    24481 gttgattcta agtataacct gatatttcca acaagagagg acgatttctt aaaaaatgtt
    24541 gatgagatgg agctgattag ggagtgtatt caaaatgaaa aattagattt aaattcagat
    24601 gcaaatacac ggatatctac cagaaataag gattattata tttcaactgt aaaaattacc
    24661 agtatatatg gtgaacaaga caagtatctt atacttttta tagacatatc aaaaactcta
    24721 aatctagcac aaaagataaa tattgtatta atatctgtaa tgtgttttgc tggaatatta
    24781 gcaatattta cagctgtgat tttatctgag aaaattgcta aaccgataaa agagttgtgt
    24841 gagtttgcaa aaaaaatggg tcaaggagat ttcaaaagaa cctactttga cttttcagat
    24901 aaagaattag tagagctctc aactgttatg aataagagtg ctgagtatct ggataagtat
    24961 gataatgaac aaaaaatatt ttttcaaaat gcatctcatg aattaaggac accacttatg
    25021 tctattaaag gttatgcaga agctataaaa tataatgtta tagatagtaa acatgcaagt
    25081 gatataattc ttgaagaaag tgataggtta agtgatatgg ttgaagattt attatacatt
    25141 tctaaaatag ataatataac taaagatttt gaacttgtag aatgtgattt aagagaagta
    25201 ctttcaaact gtggagctag gcaaaatgta agagctataa ataaggggat aaagtttatt
    25261 tatgaatttg atgaagaaat ggttttattt gaatgtgatg aaaaaaacat ttccaaagca
    25321 tttatgaatc ttatagaaaa tgctttgaga tacgctaaga gtgaaataaa aatagtgtgt
    25381 aagtataatc aaaaaaatat agttgtgata atagaagatg atggaattgg aatcaagaaa
    25441 gaagatttac ctcatgtttt tgaaagattc tataagggag taggaggaaa tcatggaatt
    25501 ggattatcaa tagttaaatc tattgtaaat aagcatggtg ggcgtatata cgtagaaaat
    25561 ggcaaaaaag gagctaaatt tactatatta tttaaattgt aaaaaaatta gagaaaatat
    25621 agagatgttt gaagttgttt tttatatata tctgttgtat actgttgaaa aaagaacaat
    25681 attaagctga catatattac gatagttgac atttaaagta aatgcttaaa tatagatacg
    25741 aaaggaaaag taaaatctga agtgtattta ggtttaagta agcttcttat tttataaaaa
    25801 atatgtatga aagaacagct gatttttaat attacaaatt tagtaacggt tgtatttgaa
    25861 gcttttgtca tccatatgtt tttatcagac tttttaggcc agaaagaagg gtatataaaa
    25921 gttgtaagat atgctaaact gggttttata atatgtttag gattctgtaa tttaataact
    25981 ttaaacccta aaatcacaat gcctttaatt ttcatcctta ttttttcaac atcattttta
    26041 tataaaggaa atttaaagac acgattgttt actactgttt tattaagtat attctttata
    26101 ctgtcagaaa ttgtagttac aagtattttt gttttatttg taaaagaagg ctttgaaata
    26161 atgcttgaaa ataatagtat aagagtattg gcgacaattt tatctaaaat tgtctttttg
    26221 ttgacttgta aaattatttg tctatttaaa aaagatgtac atttagatat gcccataaag
    26281 tattggctcc ctttattttt aatacctata tttagtttat ttctgtctgt ttctatcttt
    26341 gatgtaagta aattttttag tcttgaaagt ttaaaatttc taagcttaat atctagtgtt
    26401 ggtattttat atattaattt tattgtcttt tatttattta aatttattat agataaaact
    26461 aaactatcta tgaaatatga actacttgaa aaagaaataa ttcataagga agaattaaga
    26521 cttagtaatg agtgttataa aattattgtt gaacagactg attctgtcgt tttcgaatgg
    26581 aatatcaaag aaaataaatc atttgtatcg caggcttgga ctgaaaaatt tggatataac
    26641 aatgcatgta agaatatttt taaagaaatt aaagataaag acttagttca ttctgaagat
    26701 aaagctatat ttgaaggttt tcttgaaagt attaaaaaaa agaatatgca caatcaagca
    26761 gtatatcgtt taaaaaaatc taatggtgaa tatatttggt gtagaacatc aattacaagc
    26821 atttataatg atgaaaatga acttttaaga gttgtcggag taattgtaga tgtagatagt
    26881 gatataaaaa aatatgaaga gttaagaact agagcagaaa gtgactcatt gaccaatata
    26941 tacaataaag gtacatttga aaaattagtg gaagaaacta tagttatgaa tacaggtgat
    27001 aaaaaagatg cattattcat tattgatttg gatgatttta aagaaataaa tgataatttt
    27061 ggtcatccat ttggtgattt tgtactaaaa acatttgctg ataagataca aactagtttt
    27121 ggtagtaaag acttagtcgg aagaattggt ggagatgaat ttgttgtata tatgcaagat
    27181 tatgttacag aagtaaattt gcataaaaaa gctaaagaat taaatagagt actttcggat
    27241 aattacactg atttgagttt tagttttgat gcttctgtca gtataggtat tgcaaggtat
    27301 cctcaagatg gaacttcatt ttttgaattg tttaaaaatg cagatagagc actttatagc
    27361 ataaaagcat caggtaaaaa ttcatattgt ttatttgaag aagaactata tgtacagtga
    27421 atttgaattt agattttatg tgagagtaag atttatatat acttgtggct gtctgattta
    27481 taaaatatgt tactttgtat atttttatat gtttcagtaa ggttaattaa cataagcaaa
    27541 ggtgtaagta aatttatatt agtttaagtt tacttacacc tttataaaaa tacaaataat
    27601 agattcattt acattcttaa atgattaaag ctctttacct tctaaacatg cataacatat
    27661 cgttataatt ccagtaaata agactaaaat acccattcct aagattacag tttcattatc
    27721 tattactcca aatgttttta aagtaactta tttcgctttg tatcataata atttgtctct
    27781 ttttacattt taacttctgc tgagatttga gttagatatt cgttagaatc tcgcacactt
    27841 atatcatata gaagatattc ttcataaaca aaatccccaa tacgtaaatt attctgatta
    27901 aaaaaattta ataacttttc ataagccttg taaatagttt cataactccc tttatgataa
    27961 gcagttacat aaagtccctt tggtttgatt gatacaggat atttcttgta ctctttatta
    28021 acttttgtaa ataaatatga atatgattca aagtctttat taattatatc ttcaccttta
    28081 atgattgagc caatcgtacc ataatcactc aattcatagt tgtccataaa gttaatacat
    28141 tcatataaaa aattttcttc ctcatctcct aaaaactgtt ctttatatgt aacaggtgtt
    28201 ttgaccaagt actcttcttc atgttctttt aaagtaatag tattcgcatc tatattacag
    28261 gcatttttag tgaatgaaat agtagattcc attagagctt gaatattatt tagtttttca
    28321 atttcatttt taatatcaat ggttttcttt tctaaaagct caattagtaa attaggtgtt
    28381 cttttgtcta tataagcttt aatttctttt aaaggcattt ttacttccct aagtgaagaa
    28441 attataccaa aaatgctaag ttgatggtat gagtagtatc tatatccatt ttctttcttt
    28501 aattctggtg agaaaagacc tatatcatca taatggaaaa gtgttttttt attgattcca
    28561 caaatctttg caaattctcc agttgtaaaa taattttttg aaacttgact cataattata
    28621 attcctcctt gactatcctg ttacagtata gtttatcata gtattttgta aaggagtgtg
    28681 attatatgaa aaaattaatt cattttttaa aaccatatag agttttaatt gttgtagttt
    28741 tgatatttac gtttttacaa acacttggaa ctttgtatat accaacactt acagcaaata
    28801 tagtaaacaa tggtgtagta aagggagata tagattatat tgtaaaaaca ggtttaatga
    28861 tgatgatagt agctggtata acagctttat cagctgtgtt agtttgtaaa gtatcagcaa
    28921 atctttcatc tggattttgt agagatatta gagaagcagt atttataaaa tcacaagatt
    28981 tatcaataaa tgattttaat aatataggta cagcatcaat gattactagg agtactagtg
    29041 atattacttt aataggtcag tctgtgttta tgtttattca attagtatta ccagcaccaa
    29101 ttataactgt ttctggtcta ttcttagcat attctataga taaagcaatg acaattatta
    29161 ttgttgtagt aatgttttta ttcatgcttt cagcattttt agttggtaaa aagcttataa
    29221 agttgtttaa aatgatgcaa ataaaaatgg acaatatgaa tagggtgtta cgtgaggtag
    29281 taactggagt aagagttata agagctttta ataggagtca ttttgaaaaa aagagatttg
    29341 atagaactgc aatagattat tctgaaacag caatttcaat aaataaaata tttgcagtac
    29401 ttatgcctat tgtaatgtta ataatgaatt taggtatagt aagtattatt tggtttggtg
    29461 ggatgagagt cagtaatgga aatatggaaa taggtcatat aatggcactt gttgaatatt
    29521 gtatcttaat tttattctat ttaatcatgg gagtaatggt atttatgtac atacctagag
    29581 caggtgcatg tgcagataga gtaaatcaga tattagatat agaacctgaa attgtagatg
    29641 gaaatggtca taaagatact gtctcagaga gaggacattt agtatttaaa aatgtaacct
    29701 tttcatatgc acaatcagaa gaacctgtgt taaataatat aacatttgaa gcaaaatctg
    29761 gagaagtaac tgcaattata ggaagcactg gctcaggcaa atctactatt gcaaatataa
    29821 taccacgttt ttttgaaatt caatctggag aaatatctat aaatggtcag gatattaaga
    29881 agattcctca aaaagaactt agagataaga ttggatttgt accacaaaaa gcttttttat
    29941 ttagtggaac aatagaagaa aatataaggt atggaaaaga agatgcaagt attgaagaag
    30001 taaagcatgc tgcaagtata gctcaagctg atgaatttat ttctgatatg gaagacaagt
    30061 atgactcatt tgtagcacaa ggtggaaata atttgtcagg tggtcaaaaa cagagaatat
    30121 ccattgcaag agcgttagtt agaaagcctg aagtatatgt ctttgatgat agtttttctg
    30181 cattagattt taaaacagat aaaagactta gaaaagcgtt gaaaaatgaa atcaaagatt
    30241 catcagctat aattatagca caacgtataa gtacaatcat ggatgctaat cagattatag
    30301 ttttaaatga tggaaaaatt gtaggtattg gaaaacataa agatttactt gaaaattgtg
    30361 aagtttataa gcaaattgcg gattcacaac ttagtaaaga agaacttgca taaaatgaag
    30421 aattaatctg atatttggag gtgtatgata tgagtgaaaa aagaatagat gaacaagata
    30481 tagaagtact tagttttgaa gatggcatgt cagcagaaaa agctaaggat gcaaaaaaaa
    30541 ctactaagag attattaaaa tatatggcaa aacaaaagtt taaaatatta attattttta
    30601 tttcagtatt gataagttca gcgcttacag ttttagcacc tatggttatg ggaaaagcta
    30661 ttgaccaact atttaatgga ataaaaactg cagtccaaac aggtacaaaa ttcagtgtga
    30721 attttagtac tatggggggg atagtatcaa tacttctagg gttgtactta attagtgcag
    30781 tatttatata tattcaacag tatattatgt caggagttgc acaaaatcta gttcttagca
    30841 tgagaaaaga tttaagtgat aaactcaata aacttcctct taagtactat gattctcata
    30901 aaaaggggga aacacttagt atagtaacaa atgatttaga aaaagtagca gatagtttac
    30961 aagaaggatt aatgcaactt attacagcag tagttacagt agttggttct attgtaatga
    31021 tgataagtat aagtgtacca cttacaatag tatcagctat aacattactt gtttcattgg
    31081 gtattactgt tgtaattgca agaaaatcac aagaaagatt ctctgaaaat caaaaggcac
    31141 taggagagtt aaatagcaat atagaagaga tatttactgg tcaaatagtg gttaaagcat
    31201 ttagtaaaga aaaggatact ataagaaact ttaaaatatt aaatcaaaat ctttataatg
    31261 caagtagaaa agcacagttt tcgtcatatg caatatctcc aattattaga tttataaacc
    31321 taattggcta tgttattata gcagttgtag gtgggatatt tgcatcaact ggagcaatga
    31381 ctcttggtag tatacaagcg tttattcaat atgtaaatca agcatcagag cctactacag
    31441 aaatatctta tattgtaaat atgcttcaag cagcaatagc atctgcagag agagttttta
    31501 cagttatgga cgaggtggaa gagataaagg atgaagaatc ttcaaaggtt attagcatgc
    31561 ctaaaggtaa agtacaattt gagcatgtta aatttggata tagtgatgat tttattctta
    31621 tgaaagatat taatatcaat ctaaatgcag gagataagat tgcaattgtt ggacctacag
    31681 gagctggaaa gacaactctt gtaaatttac ttatgagatt ttatgagata caaggtggaa
    31741 gaattactat tgatggtgtt aatataaaag atttaaagcg tggagaactt agaactatgt
    31801 ttggtatggt tcttcaagat acatggctgt ttaatggaag tataaaagaa aatattgcat
    31861 atagtaagtc agatgccaca atggatgaaa ttgtaagagc agcaaaatca gcaagagtag
    31921 accattttat aaggacttta ccacaagggt atgacactat attgactgag gatgcttcta
    31981 atctatctca aggtcaaaag caacttctaa caattgccag ggcaatactt tcagacccat
    32041 cagtattgat tcttgatgaa gcaacatcta gtgtagatac tagaacagag gtagaaattc
    32101 aaaaggcaat gaataacttg atgaagggaa gaactagttt tgttattgca catagattat
    32161 caactattcg agatgctgat ttaatattag taatgaaaga aggaactatt attgaacaag
    32221 gaagccataa ggaacttatt gaaaagaaag gattctatga agaactttat aacagtcaat
    32281 ttacaagtga atatgatgaa gatgttgtat gattatcaag ttttaggcaa ctaaatgatt
    32341 gataaatatt aagttaaata tagtaacatg taaggttata tggaataaga gtatttctct
    32401 atttcatata acctttttta tttatcaaaa taagataaaa tttcctttct aaattagatt
    32461 gaagaatgta tttatcaata taaaagtaaa ataatcatta ttattagtga agtaaatatt
    32521 gtttgccaca tatatgccat aaagatgcac ttattttacg gagatttaac aaagtagtgt
    32581 aaagtatact ctaaatataa aataaatcaa atagagttaa ataaattgag aggtgatata
    32641 aatagtgaat aaaaaattga actatagatt tgaaatgaag cacaaaataa ctgaagcaga
    32701 tgtgttagct ttaaaatcaa gactctatca aataatgaag aaagatgaaa atgcatctaa
    32761 agatggaaaa tatcttatta ggagtttata ttttgatact cctgaagata aagcactttt
    32821 ggataaatta aatggcgttg caataagaga aaagtttagg ataagatttt ataataatga
    32881 ctgctcttat ataaggctag agaaaaaaat caaacattac aacatgacat caaagctatc
    32941 aactaattta accaaaaaag aagtaataaa tatactaaat aatgatattg aatttctaaa
    33001 agagtcgaca aattgtttat taagagaatt ttatttgaaa ttaaaatgtg aaagattaga
    33061 ggcaaaaagc atagttgatt acaatagaga agcttttatt tatccagttg gaaatgtaag
    33121 agttacaatc gatagtgata ttaaaacttc tgtaaattcg gtagatttat ttaataaaga
    33181 tttgccaact gtttcggtaa ttgatgagaa tatgactgta ttggaggtta aatatgatga
    33241 atttatacca gattttataa aggatttaat tcaaattaat aagactacct ctacagcagt
    33301 ttcaaagtat gcttcaagta gattatatat ttaagaataa aggctatgat ttatataaag
    33361 atatacataa ataaggttaa aaaacaaata agttaaaaat aaataaaata aaaaataaat
    33421 aagttaaaaa taggaaggat gattttaatt atgacattta atgatatttt taaatcaagt
    33481 tttatagaaa acgtatcagg tttttcattt gtggacagtg ctttagcact aggttcagct
    33541 tttttagtag gtttgtttat atatatggtc tataaaaaga cttatatggg aattatgtat
    33601 tctcgaccat tcaatgtgtc gcttgtagca cttacaatgc ttacaacttt tgtaatattg
    33661 gcagttactt caaatgtagt tttatcctta ggtatggttg gtgctttatc tatagtaaga
    33721 tttagaacag caattaaaga cccaatggat ttagtatttc tattttggtc attgggttca
    33781 ggtatagttt taggagcagg tttgattccc ttagctgtca tgggttcaat aattatggga
    33841 ttgattctaa tattcttttc aaataaaaca atatctgaaa ctccatatat attgatggta
    33901 aattgtaata atgaagattc agaagatata gcaactgata aaattaaaaa agtatttact
    33961 aaatatcaaa taaaatcaaa aagtgttaca ccagaaaaag gaatagaatt agtgtttgaa
    34021 gtaagaatga aagatggaga aacaagtctt ataaatgact tgagtcaagt agatggagtt
    34081 actaatgcag ttcttgtaag ttataatggt gactatgtag catagagagg agtaatcttt
    34141 tatgaaagat aaaaaattta cccttcttat ctcgattatg attatatttt tatgtgctgt
    34201 agttggagtt tatagtacat ctagcaacaa aagtgttgat ttatatagtg atgtatatat
    34261 tgaaaaatat tttaacagag acaaggttat ggaagttaat atagagatag atgaaagtga
    34321 cttgaaggat atgaatgaaa atgctataaa agaagaattt aaggttgcaa aagtaactgt
    34381 agatggagat acatatggaa acgtaggtat aagaactaaa ggaaattcaa gtcttatatc
    34441 tgtagcaaat agtgatagtg atagatacag ctataagatt aattttgata agtataatac
    34501 tagtcaaagt atggaagggc ttactcaatt aaatcttaat aactgttact ctgacccatc
    34561 ttatatgaga gagtttttaa catatagtat ttgcgaggaa atgggattag cgactccaga
    34621 atttgcatat gctaaagtct ctataaatgg cgaatatcat ggtttgtatt tggcagtaga
    34681 aggattaaaa gagtcttatc ttgaaaataa ttttggtaat gtaactggag acttatataa
    34741 gtcagatgaa ggaagctcgt tgcaatataa aggagatgac ccagaaagtt actcaaactt
    34801 aatcgttgaa agtgataaaa agacagctga ttggtctaaa atcacaaaac tattaaaatc
    34861 tttggataca ggtgaagata ttgaaaaata tcttgatgta gattctgtcc ttaaaaatat
    34921 agcaataaat acagctttat taaaccttga tagctatcaa gggagttttg cccataacta
    34981 ttatttatat gagcaagatg gagtattttc tatgttacca tgggatttta atatgtcatt
    35041 tggtggattt agtggttttg gtggaggtag tcaatctata gcaattgatg aacctacgac
    35101 aggtaattta gaagacagac ctctcatatc ctcgttatta aaaaatgaga catacaaaac
    35161 aaaataccat aaatatctgg aagagatagt aacaaaatac ctagattcag actatttaga
    35221 gaatatgaca acaaaattgc atgacatgat agcatcatat gtaaaagaag acccaacagc
    35281 attttatact tatgaagaat ttgaaaaaaa tataacatct tcaattgaag attctagtga
    35341 taataaggga tttggtaata aagggtttga caacaataac tctaataaca gtgattctaa
    35401 taataattct aatagtgaaa ataagcgctc tggaaatcaa agtgatgaaa aagaagttaa
    35461 tgctgaatta acatcaagcg tagtcaaagc taatacagat aatgaaacta aaaataaaac
    35521 tacaaatgat agtgaaagta agaataatac agataaagat aaaagtggaa atgataataa
    35581 tcaaaagcta gaaggtccta tgggtaaagg aggtaagtca ataccagggg ttttggaagt
    35641 tgcagaagat atgagtaaaa ctataaaatc tcaattaagt ggagaaactt cttcgacaaa
    35701 gcaaaactct ggtgatgaaa gttcaagtgg aattaaaggt agtgaaaagt ttgatgagga
    35761 tatgagtggt atgccagaac cacctgaggg aatggatggt aaaatgccac caggaatggg
    35821 taatatggat aagggagata tgaatggtaa aaatggcaat atgaatatgg atagaaatca
    35881 agataatcca atagaagttg gaggttttgg taatagagga ggaggctctg tgagtaaaac
    35941 aacaacatac ttcaaattaa ttttaggtgg agcttcaatg ataataatgt cgattatgtt
    36001 agtaggtgta tcaagggtaa agagaagaag atttataaag tcaaaataag attaaaattg
    36061 aatataaata attttaatac tgttaagtct aaatatttac ttagcagtat tttttagaaa
    36121 ataatgtgtt tgattaataa tataaggtaa taaattgata aaaattaaat acagcatatt
    36181 aataatacat aataaagtaa taaaaaactg tttaagtata ataacttata tttagacagt
    36241 ttttttcatt tataaataat ataaatgaaa ttgaaaacat ttgaaggata gaaattggtt
    36301 ggataaatgt ataaattact tcaccaaaaa ataatgacaa aaactgtaac taagttgtaa
    36361 taaaatggta gttatttagc ataaaataat gtatcataga agtatattat aaaattaata
    36421 aaataagatt tttgtaagaa aactgctggc ttttgatatt tagagtgagt agttaaatta
    36481 tgtttaggag gagtagaatt tgttgcaaaa agtattatgg aaatttttaa agctagtagt
    36541 cggtttattt atatgttcag ttggtatagt attgacgata aattgtaacc ttggattatc
    36601 tccctgggat gtatttcatc aaggactttc aaatcatata ggaataacta taggaactgc
    36661 ttctataatt gtaggctcta tagtagttat tgctgatgtc gttcttggag aaaatgtagg
    36721 atggggaact gtttttaaca tgctactaat tggatttttt atggatttac ttttatatag
    36781 taatttaatt ccagaggctg ataatttatt tgtaggaata attatgttga tattaggatt
    36841 ggttcttttg tctgttggaa tggtatttta tatgggaagt ggtttaggaa gtggacctag
    36901 agatggtttg atggttgcaa ttcaaaagaa aacaggaaaa tcacttaaat taataagagg
    36961 tactatagaa gttggagcat taatagtagg atttttatta ggtggaaaag ttggtatagg
    37021 aactataata agtgcatttg gattggggta tttcacacaa atggtatttg gcttatttaa
    37081 attagattgt agtaagatta aacatagatt tatagtagat gatataaaat ttatcaaagt
    37141 gtatatgagt ggtgagaaaa aagctgctgt tcaaaatata gttaagtaat ttaagaataa
    37201 attcaacgaa atgttaagta ttttaaatta acattttgtt tagatagata aattgaatat
    37261 taaaagaata ttaaaatatg aggattgcta gaacactata aaatatagat ttctagcaat
    37321 tttttatttg ttcaatattt ttttagtact agaagtctta ttctctattt aatgaatgaa
    37381 ttaatttata attagagata ctaaacttta tagatattaa tataaacacc tttaaactgt
    37441 gtattttcca aagcctttaa aatgcttgta tttattctaa ataaaaaaat aaaaaaatgt
    37501 caaaaaaact cttgtattat atccaaattt tatatataat tacctatgtt gtttagaaat
    37561 tttaaactca atgtttagta aacaaaatgt ttagtaaaat aaaacagaaa ctaggaggtg
    37621 caacatggat ataggtgaaa agataaaaag attaagaaca gaaaaacaac tgacccagga
    37681 agagttagct aatagatgtg aattgtcaaa gggatttatt tctcaattag aaaataactt
    37741 aacatcacca tccatagcta cccttataga tatacttgaa atactaggta caaatttaag
    37801 ggaatttttt aatgaaatag atgatgaaag aatcagtttt acaaaagaag atatgtttga
    37861 aactgaagat gaagatttaa aatataaatt aaagtggtta attccaaatt cacaaaaaaa
    37921 tgaaatggag ccaataataa taacattgta tccaggagga cagtataaag aagaaaagcc
    37981 acatgaaggt gaagaatttg gatatgtatt agcaggttca atatatgtcc atataggcga
    38041 aaagaaaaat aaagtaaaaa aaggagagag tttttacttt agaccgaaag caaatcacta
    38101 tatatcaaac gagggcaaaa ctacagcaaa agtgatttgg gtaagtacgc caccatcatt
    38161 ttagaataga ggtgtttaat aattggtaga aaatataata gaattaaggg atatatctaa
    38221 acattatgaa gaacttactg ttttagataa tttcaattta gatataaaga aaaatgagtt
    38281 tctaacattg cttggaccaa gtggatgtgg aaaaactaca acattaaaaa taatagcagg
    38341 atttgagtat gctgatgatg gtaaagtttt atttgaaggt aaggagataa ataatttgcc
    38401 accatatgaa agacaagtaa acacagtatt tcaaaaatat gctttatttc cacacatgga
    38461 tgtttatgaa aatgtagctt ttggattaaa aattaaaaag actccaaaag atgtaataga
    38521 tgctaaagta aaagaggttt tgaaacttgt tgcactagaa ggttttgaaa gacgacaagt
    38581 tgaatcattg agtggaggac aacaacaaag aattgcaata gcaagagctc tagtcaatga
    38641 accaaaggta ttattactag atgaaccact tggagcactt gatttaaagt taagacaaga
    38701 gatgcaaata gaattaaaga gaatccaaaa aaaattagga ataacatttg tttttgtaac
    38761 tcatgaccaa gaagaggctc ttactatgtc agatactatt gttgtaatgt ataagggcaa
    38821 aattcaacaa atgggaacac cacaagatat atacaatgaa cctaaaaatt catttgtagc
    38881 taagtttata ggtgaaagta acatatttga tggaattatg atagaagatt ataaagttaa
    38941 tttctgtaat agagattttg agtgtgttga taaaggattc gaaaaaaacg aaaatataga
    39001 agtagtaata agaccagaag atataaaaat ggtaaagcca gaagaaggaa tgttaaaggg
    39061 gaaagttaca tctacagtgt ttaaaggagt gcattacgag atagaattga atgaaaatgg
    39121 aagattgtgg atacttcata atactaaaaa tgctgaggtt gggactgaac taggcatgga
    39181 tatatatcca gaagatatcc atataatgag aaaagagaat aactaagatg aaaagaaaat
    39241 cttttttagc atatccatat gtagtttgga gtgcgatatt tgtaataatt cctttaatat
    39301 tagtagtatt ttttagtttt acaaaagaat caggtggagg ttatgccttt acacttgaga
    39361 attataaaga agtaattgac cctatatata tgaaagtttt tgggcgttct attcttttag
    39421 caggaggagc tacattaata tgccttatag ttggttatcc agtggcatat ataatttcaa
    39481 aggctcgtgt aagtagaaga ggttcattaa tattattgtt tattcttcct atgtggatga
    39541 actttctttt aagaacatat gcatgggttg ctatattagg aaaaaatgga cttttaaata
    39601 catttttagg ttggtttgga atacaaccat tagcaatttt gtatactaat tttgctatac
    39661 ttttaggtat ggtgtataac tttttaccat ttatggttct tccaatatat acagcactat
    39721 caaagatgga taatgatttg ataaacgctg ctcatgactt gggtgcaaat aatatgacag
    39781 tttttagaaa aattatattt cctttgagtt tgccaggtgt aatgtctgga ataactatgg
    39841 tatttatgcc agcagtcact acttttgcaa tatcaagact gttaggtgga ggtaaaataa
    39901 tgctggttgg agatttgatt gaacaacagt ttacagttgt tggagattgg aactttggtt
    39961 cagctatatc gatatttatg atgatagtaa tattaatatc tatgtctata atgtcaaaat
    40021 ttggagatga atctgacaaa gaaggcggtg ggttactatt ttaaaattaa agaaaataac
    40081 ttctaatata tatttagcat tagtattctt atttttgtat gcacccatat ttgctttggt
    40141 actattttct ttcaatgatt caaaatcaat ggcaagatgg aatggattta catggaagtg
    40201 gtatggtcaa ttacttcaaa atgagagtat aatgagtgct ttatattata ctattgtaat
    40261 agctatatta gcgtctgtaa tatctacaat agtaggaact ataagtgcta taggaataca
    40321 taagatgaga ggtaagagta agaaattaat actaaatgta aactatcttc ctattttaaa
    40381 tacagagata gttacagcag tagctcttat gagtctgttt gtatttgtaa aaatggaatt
    40441 tggatttaca acaatgctat tggcacatat aatgttttgt ttgccatacg ttatactttc
    40501 agttttgcca aaaatgaagc aattacctga taatatagaa gatgcagcta tggacttggg
    40561 agcaacacct atatacgctc ttagaaaagt aattttacca caaataaaac caggtatagt
    40621 ttctggattt ttaattgcat ttactatgtc aatagatgac tttataataa gtttctttaa
    40681 tgctggaaat ggtgtaagca atctttctat agaaatctat ggaatggcta gaagaggtat
    40741 aaaacctgaa ataaatgcat tatcaacaat aatgtttgca gtggttctag gtttactttt
    40801 actagctaat aaaaaagaat ctatagtaag ggggattaaa taatgattaa gtttaaaaaa
    40861 gcaatatccc ttatagctgt aggtatgatg acagttactt tatttacagg atgtaataaa
    40921 gcaactgatt ctacaaaagt tttaaatgta tataatgttg gagattatat agatgagtct
    40981 cttatagata agtttgaaaa agaaacagga atagatgttc aatattcaac ttatgatact
    41041 aatgaaatga tgtatcaaaa agtaaagagt ggtagtacac actatgattt agtatttcct
    41101 tcagactata tggtagagaa aatgaaaaat gaaggtcttt tggaaaaatt agattttaag
    41161 aatataccta atatgaaata tttagataag agttttttaa atcctgtata cgacaaaact
    41221 aatgaatata gtgttgcata tatgtgggga actcttggaa tattgtacaa taagaaagaa
    41281 gttaaagacc ctatggatag ctggaatata ctttggaatc caaagtataa gggaaatata
    41341 atgatgtttg actctgttag agatactata gggataactc ttaaaaaatt aggatatagc
    41401 atgaatagtg taaatccaaa agaaataaat gaagctaaaa atcttttaat gaaacaaaag
    41461 gatttagtat tggcttatgt aaatgatgaa ggtaaggata gattgcttgg tggagaagtg
    41521 gcaatgggta tgctgtattc tggagatgca gttacactaa tggagcaaaa tcctgacctt
    41581 gattatgcta tacctaaaga gggaactaat aaatgggtag atgctatgtg tgtacctaca
    41641 acagcacaaa ataagaaaga agctgagctg tttataaact ttttattaga cccagagaat
    41701 gcaaaagtaa atgctgagta cattgggtat tcaacaccta atacaggagc actaaaatta
    41761 ttagacccag aaataactca gaatcctgtt gcatatccat ctaaagaagt acttgataaa
    41821 tgtgaaactt ttgaagatat tggtgaaaat ataaaattat atgacaaggc ttggatagag
    41881 ttaaaatcaa aatagggtct tgttgtttta agtaaattta cttaataaat acttaataaa
    41941 actgttcttt tttagaaaat ttattttcta attattgaac agttttattt tttgtttaag
    42001 ttatattcat atgcaattta aatagtttat taataaatga aaacttattt gtataatgac
    42061 tttaataata attagaaaaa atgggcaact taatagaata attggaggtg taaatttata
    42121 aaaaatgaat aggataaata aaaaaataga tttattaata ttggctttgc tcgtttttat
    42181 atttttaatt gttggaatag taatactatc gattaggaca gaaaataaat tgaatgtgtt
    42241 cataatgtta gcaataattt tttttataat aatgttaact tatttatcaa attcagtagt
    42301 ggggttgata acaagttcaa taattatatt tatgtataca tcctatattt tatataacaa
    42361 tattactcac aatatggatg ttgaatttat atcatatatg tggataatag caacaccagt
    42421 atcttcaatt ataatgggta atctgaacaa aagcataaat gaattgcaaa atacaaataa
    42481 aaaactatct gaacagtata aggaattagt gactattgat agtgaaactg gacttagaaa
    42541 tttaaagatt ttttataatg atgtaaatat ggagataagt aaatctatta gacataatac
    42601 tgatttttct cttatgatag taaaattacc atactacgga aacctacaga ctatatttgg
    42661 tgaaaacaaa actaataaaa tagtaaaata tattggttca aatattatag aatgtacaag
    42721 aaatgaagat ataatttata gtttacaaaa agatatgata gggatactaa tgccaaatac
    42781 atctttggaa ggttcaaaag ttgttaaaga cagaataaag aaaaggataa aagaattaaa
    42841 tttagattta aacaataggg gaaaatatgt aaatatagat gtcaaaattg catttttaca
    42901 atataagagt agttttggag atagtataaa ttttaagaat atagttgaag aggaacttca
    42961 atatgatgtt taaaatattt aataaaagtc tattaaaatt agtagttgta ttatcaatag
    43021 tactgacaat atcaaatttt agcttatctt catatgcatc agaaattact tccagcacag
    43081 ataaggtatt aatcatatat gattctaaga aagaaacagc ttataataga gatattttaa
    43141 atattatgag gactctattg ggaagattta gttcagatat agagctatta aaattgagta
    43201 actatgatgg tgagataaat aaaaattatt acagtcatat atttatttta ggaatcaatg
    43261 aaaactcata taataatgat aaaaacacta aaaacttgat tagttcctta aattcatata
    43321 aaggaactat atgttggtta ggatatggta tagaaaatct tttggaacat aaaaaataca
    43381 atttagacta tgtaggaaaa acaaacaata tagttagtgt aaattataga ggtaaatcat
    43441 ataacttgga tgaacattat gtttttaaca ttgtagaatc aaaagataca agtaataaag
    43501 taataggaag tattaatgat accttaaata agtatccata tataataaat gataaaaatc
    43561 ttttttatgt atcaaaatta gatttagatg gagttttatt ttatatattt tgcgattcac
    43621 ttaatgatat ttttaatatt aagacgtttg ataaaggacg tatatttgta agaatagaag
    43681 atgtacatgc ttttagagaa ccaaaaaatc ttgtagagat agcggattat ttaagtagta
    43741 agaatatacc atttacgatt gcattaatac cagcatatgt aaatcctaaa aatcataagg
    43801 taataacttt atcagaaagc cctgaaattg tcaaagcaat aaaatatatg caagataaag
    43861 gtggaactgt gatattgcat ggatatactc atcaatacaa gaaagaagaa gtttctggag
    43921 aagggtatga attttgggat ggtaaaaaag atgaaccctt aaaagaaaat atgaagatat
    43981 ttgtcaaaga tagagttttg aatggtctta gagtatgtat agagaatgga atttatcctc
    44041 ttgcatttga agcgccacat tatgcaatgg aatcagaggg atataaagaa ttaaaaaaat
    44101 atttttctac ttatatgggg caacatcaaa ataatgataa aaaatttagc acaaatacat
    44161 atccttatat tattagggat acagaggagt ttaatatatt tatacccgaa aatttaggat
    44221 atatagaccc agaagataag tttacttttc aaaatataaa agaaaattta aataaattat
    44281 ccattgtaag aggttttagt ggagggttct tctttcattc atatttaaac atagagtatc
    44341 taaaaaacac aatagaatat ttagaaaaac agaatataga atttatgaat ttaagagatt
    44401 ttaataattg ggtaaaagtt gatgaaatac aaataagaaa taatggagat gaaattatag
    44461 tcaactatga taaggactta gatgaaataa ctaaaagtga tactagattt aaatccatat
    44521 ccaatattag taaagtttta atattcatag tatctataag tgttttaata tttgtgatta
    44581 tatttatata ttttaagaga atagataaaa agaaattttt aaaataaatg attttatatt
    44641 agaatcaata ttttaaaata ttttaggaga aatttatgag agatttaaat gtatcagagt
    44701 atttgttcgt atttagtctg tttagtatat ggtctttatt gcttataaat attattttag
    44761 ctatgggtgg atatatattt tattttaaaa actttgataa agaaataaaa gaaattgatg
    44821 aatatcctat gatatcaata cttgttcctg ctcataatga agctaaagtg ataggtagaa
    44881 ctgtggagtc tttgctatta ctcaattatc ctaagagtaa gatggaactt attgttataa
    44941 atgacaattc ttctgataat agtaaagaaa ttttagaaaa tataaaggat aggtacaaca
    45001 attataattt cactataata aatacagata gtttaacagg tggaaaagga aagtctaatg
    45061 cattaaatat tggatatacg atttcaaaag gagattttat agctgtttat gatgcagata
    45121 acacaccaga taaaaatgca cttagatatc tagtacaaac tatagtaatg aatgatgaat
    45181 taggtgctgt tattgggaaa tttaggactc gaaataaaaa taaaaaccta ttaaccaaat
    45241 ttataaacat agaaacatta agctttcaat ggatgtcaca agctggtaga tggcaacttt
    45301 ttaatctatg tacaatacca ggaactaact ttatattgag gagaagtatc atagaagaaa
    45361 ttggaggatg ggatagtaaa gcaattgcag aagatacaga aataagtttt aggatatata
    45421 aattgggata caagataaag cttgtaccac aatctataac atgggaacaa gagccagaaa
    45481 ctgttaaggt gtggataaag caacgtacta gatgggcaaa agggaatata tatgtattaa
    45541 tgaaatatat aaaaaatata tttaaacagg gaagaaataa gattgtgttt gatatagctt
    45601 actttttttc tgtatatttt ctatttttaa catctgtaat tatatccgat attttatttg
    45661 ttttaagtat atctaaacta gtagaaattt ctattccaat taactttttt ctaatatgga
    45721 tactatctta tcttttattt attatagaag tgagtataag ccttactata gaaaaaggtg
    45781 aagcaacaat tgaaaatata ttcatagtgg caataatgta ttttacatac tctcaactat
    45841 ggctgtttgt agctataaaa ggaatgatag agtatctaaa agatataatt tttaaaagag
    45901 aggttaaatg gtataaaaca gaaagatttt gattcatgta caggaggaat taacatgaaa
    45961 aaatttatta tatcaattat atctttagta ttgttttttt caaatatatc acttatatac
    46021 aaagtaaatg ctgatgagac taaagttaaa aattataagt ttgaaagaga tataactatt
    46081 gatggtgtta taggaagtaa tagtactttt tttgaagtaa ataaaaattg ggatattgaa
    46141 gaagttttat tgcatcttaa ttttagtaag agccaaatat taaatggaga tgtttcaagt
    46201 ttaactgtat tgataaataa tgtaccaata aaatctatta aattaaatgc taagacaaat
    46261 tataaaaata cattagaagt attagttcca aaagattata taattcaagg atataatgaa
    46321 ataaaaataa aaacatacaa aacaatatct gataaaattt gccaagatga ttccaatact
    46381 gggaactgga tggttattca caaagaatca tatatatcaa ttagatataa gcagaaaaag
    46441 gtggaaaact caataaatga atatccgtat ccatatgcag aaattgaaaa taatcataaa
    46501 ttagatacaa ctatagtagt accagataat atgactagag gagagactac agcagtgttt
    46561 aatttggcat cagcatttgg aaaaataaca aaaaatgatg atttaaagtt agatgttaaa
    46621 ttatattcag aaatgaaaaa ttggtcagat gataatatta tatatattgg taaacctgaa
    46681 aatacagcag aagaaatttt ggatatttta agtataaagg aacaaaccct attatcttca
    46741 aactgtataa tcaagcaagt agattcacca tacaataaga ataagaagat gatggtggta
    46801 attggtagta atgaagatga tttaataaag gcatcaaatt tattaataga aaatagactt
    46861 tctaaccaag ttttatcatc atcagtactt gtaaacaaag agactaatat aaaaattaat
    46921 agggaacaaa aattaaattt aggtcattta acattaaaag acttaggata ttctgacttt
    46981 ttgttggaag gagcatttaa tcaacaagct ttgtttgatg tcaaaatacc aacgggtaag
    47041 gtattagatg atggttcaaa gattatctta aatttaagat attctgacaa tttagatttt
    47101 gaaaaatctt tagttacagt gtctataaat gatgttattg tgggaagtaa aaaattggat
    47161 aggtcacatt ctaataatga taagttagaa ttaaaaatac ctaaagatat agataataaa
    47221 aactattatc aagttaaatt gacatttaat ttgagtataa aaaattcgaa ttgtgttact
    47281 agagagagta ataatccttg ggcatatgta tcaaataatt cttatttggc gttatctatg
    47341 aaagaaaatg aaaccttatc ttttgaaaat tatccgtatc catttgttag agatgatgaa
    47401 tttaatgatt taactgtgat aatgccagat tattcagggt cacaagctat gacttggatg
    47461 tttagattag gtgttacttt aggtgcgaat ataaattcac ataatggaaa tataaatgta
    47521 attagaggaa aagaatttag tgacaagtat aaggatacta atattgtagt ttttggagtg
    47581 ccacataata attctgttat aaagatgtta aataataatt taaatataaa attcgataaa
    47641 aattactcca attttatatc aaatgataag ataagcttta ttgatgatta tggaaaaaat
    47701 atttctacca ttcaattaat aaaatcacca tataataatc aaaaaaatat catggtaatt
    47761 agttcaatga atgaaaaaaa cttatattta ggaatggatt atttgttgaa taaaagtaaa
    47821 gttaatgatt taaaaggaga tacattgata attgatgaat acggagaggt agaagattta
    47881 gcttataatt taaaatctaa gaaagaagta aaagattcaa gttggaatat gagtataaat
    47941 aagacaacta aagtattttt aatgatttct tttattacta taattgtagt catgatatta
    48001 tcaatgctat atataaaaaa atataaaaga agataatatg ataaatttat tattttaaaa
    48061 gaggtatata atttgaggaa atatatgaat aaatttaaat taaaaagaag tattttaatc
    48121 tcattaataa tcttaatttt tatatttata gtttactttg taagtattga gttaaaagat
    48181 aggagtctag taaataatca atataaaggt aagattaaat cggccaattt atctgtagat
    48241 tatgaaataa atcaagttat gaaggatata gataaattgg gattgaatac agtaaatgta
    48301 cctataatta taaatgtaga gtccattaat tctgatatta tgtcaataga taataatagt
    48361 aaagaaaagg caattaagtt aataaaaaaa ttaaataaaa aaggtatttc aacaatatta
    48421 gaggcatatc catggattga aaatggaaaa ttgtatgaga ctgactggaa ccctataaat
    48481 aaaaaaagat ttttttatac ttggcaaaat gccatattaa atgaacttat aattgatgta
    48541 gcaaatccac tggatgtaaa tgttttaaat attggttcta attttgtaca tttggaagaa
    48601 tatcaacaaa attggggtga aataatagat tttgtacaat ccaaatttga cggttttgta
    48661 acgtacagaa ctaattggtg gtatacaaaa aaagatgatt taagtagtaa gttgttttat
    48721 gaacaaaagc tgaacaataa tttttttgat aagttagatt ttatatctat agcagcatat
    48781 tttgagttaa gtaataaacc tgttaataca gtggatgaat taatttctgc cttacactca
    48841 tctactgtaa acaatagagg acaaaatata aaacaagaaa tatataacct ttataaagct
    48901 catagaaaac ctatattttt tggagaatta ggtttttcaa atagagaaag tgcaagttct
    48961 cagccgtgga atcatactcc ttcaaaagct gtaaatggcg aagaacaagc aaggtgtttt
    49021 gaggcatata agaaagtttt tgaaaatgaa gactggataa atggtttttc tgtgttttgt
    49081 gtaggtaaaa tagatgacga aaaaaatttt tacccatcaa aagagagtat aaaagttatt
    49141 aagtcttggt atgaataatt ttgaaagtgt agaatttata tagattaaat agatgattag
    49201 gagagacata aatgaatgac atcaaggtta tgactgtatt tggaactaga cctgaagcta
    49261 tcaaagtagc acctttaata aaggaacttg aaaaaagaga gaatataaaa agtattgttt
    49321 gcgttacagc tcaacataga gaaatgcttg accaagttat agaaaccttt aatataaatg
    49381 tagactatga tttggatata atggaaaaag gacaaagcct gaatgatata acatgtaaga
    49441 ttttaaataa acttcccttg attttaaaca aggaaaatcc aaatataata ttggtacatg
    49501 gagatacaac cacaactcta gctacaagtt tgactgcatt ttataataaa actcttgttg
    49561 gtcatataga agctgggctt agaacctatg ataaatattc tccttttcca gaggagttaa
    49621 atagacaact tacaggtatt attgctgata tgcactttgc tcctactaat ttggctagaa
    49681 aaaacttgat ttctgaagga aaacctaata ataatatatt tgtaactggt aatactgcta
    49741 ttgatgcttt aaagatgaca ataaaagaaa actataatca tcctataatt gatgagattg
    49801 gtaacgatag gatgatttta ctaacctctc acagaagaga aaatttgggt aaacctatga
    49861 aaaatatatt tagagctata aagcgaatag tagatgactt tgaagatgta cagatagtat
    49921 atcccataca tttaaatcct aaaatacgaa ctatagctga tgagatattt ggaaaatttc
    49981 ctgaaaaaat tcatattata gagcctttag atgtagctga ttttcataac tttttaaata
    50041 aatcttatat gataatgact gatagtggag gaatgcagga agaagctcca tcattaggta
    50101 agccagtcct tgtgttgcgt gataaaacag aaagaacaga aggaattgaa gcaaagacac
    50161 ttaaattagt aggtactaat gaagatagaa tatataattc agtgagcgat ttattaataa
    50221 ataaagataa ctatgttcag atgagtaaag catctaatcc atatggagat ggaaatgcaa
    50281 gcaaatatat agtggatata ataattaaaa aatttaattg caaatattta aattagataa
    50341 gacctaaaac attaactaat tgatataaga tttatgataa tattaaggaa tacaattttt
    50401 aagggggagt aatatggcta gaaaattaat aaaaaatttg ggtaaaagta aaagtgtaaa
    50461 gagagtgaaa cttttattta agaagatttt tattactgta ttcatagtag caagtatagt
    50521 tgctattttt aatataacga aatattttga ggaattatac aaagtaaggg atttaaaaag
    50581 tactaaaatt gaatactata tggatgtggc tgatgaagca ggagatggaa aggtccaatt
    50641 aagttggaag gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat
    50701 aaaaaagaaa gacacattgg atatagggga aaagtttata gtagaagata aaaatgataa
    50761 aggcgaaaag gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga
    50821 ctcttctcaa aaaagtagag caagaaaata catgaaagat ttagaataca cataccttgg
    50881 aaataaacaa ttagatagta gtgatgaaaa aattaaattt ataaagaagt tagaagactc
    50941 agctataaga gaatatattg attatggaat attgccctct ataacaattg gacaagctat
    51001 attagaatct ggttggggaa attctaaact tacaaaacag agtaataatt tatttggtat
    51061 aaaagcagat aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa
    51121 tgaaaaaatt gtagctagtt ttaggtcgta taattcatta caagattctg tcaaagacca
    51181 cagtttattt ttaattaata ataaaagata tagaaaacat ggattgtttg aggcaaaaga
    51241 ttatattagt caagctcaat cattagaaaa tgcaggatat agtacagcag aagataaaaa
    51301 aggaaatcgt atatatgcag agctattaat agatgttatt aggagttata atttgcaatt
    51361 gatagataat aaagttgaga caaaataatt gttaacttta tatgaggaga taacaagata
    51421 agattctttt ctacaaaaag aatagaaaaa ttgtaaaaaa aactaaaaaa atgtattgac
    51481 ttgttattta aaaggtgata ttattattac tgtactcgaa agagaacaaa a
//
LOCUS       DAMJVI010000015        50630 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.015, whole genome shotgun sequence.
ACCESSION   DAMJVI010000015 DAMJVI010000000
VERSION     DAMJVI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 50630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50630
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.015"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(174..2144)
                     /gene="ftsH"
                     /locus_tag="PC545_000981"
     CDS             complement(174..2144)
                     /gene="ftsH"
                     /locus_tag="PC545_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2455770.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(2178..3566)
                     /gene="tilS"
                     /locus_tag="PC545_000982"
     CDS             complement(2178..3566)
                     /gene="tilS"
                     /locus_tag="PC545_000982"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDF2455771.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERNEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVNEE
                     IEFYYNVPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(3749..4177)
                     /locus_tag="PC545_000983"
     CDS             complement(3749..4177)
                     /locus_tag="PC545_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDF2455772.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVI
                     YKEKEEDIEITNTIKILKNQVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RELDINIQEGEHIKLKIDYNIKVQDLFLGRNKIDIYIGTK"
     gene            complement(4194..5000)
                     /locus_tag="PC545_000984"
     CDS             complement(4194..5000)
                     /locus_tag="PC545_000984"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDF2455773.1"
                     /translation="MNNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKI
                     LEVQNIINNTEIHGTYQYYVSDIPEKFSDIAREFLKKKIDKIQKVEIQKY"
     gene            complement(5202..5687)
                     /locus_tag="PC545_000985"
     CDS             complement(5202..5687)
                     /locus_tag="PC545_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HDF2455774.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTENIKKVNKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(5738..6397)
                     /gene="spoIIR"
                     /locus_tag="PC545_000986"
     CDS             complement(5738..6397)
                     /gene="spoIIR"
                     /locus_tag="PC545_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HDF2455775.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILKNGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVLEVVKDLEKK
                     F"
     gene            complement(6542..7222)
                     /locus_tag="PC545_000987"
     CDS             complement(6542..7222)
                     /locus_tag="PC545_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455776.1"
                     /translation="MENLTKLNLDNYKPLRDVVFENLREAILEGKLKPGQRLMEVQLA
                     EQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLEVRASLEGLAAYL
                     AAERISDEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKATNNKKLIQLINS
                     LWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYATEHIEKAEQFMID
                     KAMISKDL"
     gene            complement(7206..8096)
                     /locus_tag="PC545_000988"
     CDS             complement(7206..8096)
                     /locus_tag="PC545_000988"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDF2455777.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELDEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNHLWELRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTNIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTIGKHKEISDIKQVMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(8214..9764)
                     /locus_tag="PC545_000989"
     CDS             complement(8214..9764)
                     /locus_tag="PC545_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HDF2455778.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(9858..10124)
                     /locus_tag="PC545_000990"
     CDS             complement(9858..10124)
                     /locus_tag="PC545_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDF2455779.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(10272..11132)
                     /gene="yabG"
                     /locus_tag="PC545_000991"
     CDS             complement(10272..11132)
                     /gene="yabG"
                     /locus_tag="PC545_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HDF2455780.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(11216..12154)
                     /locus_tag="PC545_000992"
     CDS             complement(11216..12154)
                     /locus_tag="PC545_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HDF2455781.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMANKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLKKFYDLGVRMMTISWNHVNELSFPHNKVEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDDIEKIYYKNALRVIKEVL"
     gene            complement(12264..12425)
                     /locus_tag="PC545_000993"
     CDS             complement(12264..12425)
                     /locus_tag="PC545_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455782.1"
                     /translation="MFILRFADDDDNLSVKDFTSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(12435..12932)
                     /locus_tag="PC545_000994"
     CDS             complement(12435..12932)
                     /locus_tag="PC545_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2455783.1"
                     /translation="MSLYTEWRNLSDNHESQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEEVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(13047..13757)
                     /locus_tag="PC545_000995"
     CDS             complement(13047..13757)
                     /locus_tag="PC545_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HDF2455784.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNDVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWAKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYDVDVNNLKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFDLEVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     NQEYAIKVLKELFNYDII"
     gene            complement(13933..14463)
                     /locus_tag="PC545_000996"
     CDS             complement(13933..14463)
                     /locus_tag="PC545_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455785.1"
                     /translation="MDIRALPIRITKEEAIEIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIITLKIHKTKSIFKNRRYSNDINELKILVNGSTGSGSIVQDFPTYEVVKNVDCN
                     IVQYSDKDERRVKAKANKMAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(14480..14710)
                     /locus_tag="PC545_000997"
     CDS             complement(14480..14710)
                     /locus_tag="PC545_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455786.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGKIIPYGVMSVVIVIFFDYIYRVLKMTRKK"
     gene            complement(14725..16119)
                     /locus_tag="PC545_000998"
     CDS             complement(14725..16119)
                     /locus_tag="PC545_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2455787.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWKIDKELIGLIMVGV
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(16245..17498)
                     /locus_tag="PC545_000999"
     CDS             complement(16245..17498)
                     /locus_tag="PC545_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2455788.1"
                     /translation="MESKNNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALALS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGILGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGILLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKGLNNKIA"
     gene            complement(17549..18703)
                     /locus_tag="PC545_001000"
     CDS             complement(17549..18703)
                     /locus_tag="PC545_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2455789.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKEAMRPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEENVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(19263..19832)
                     /locus_tag="PC545_001001"
     CDS             complement(19263..19832)
                     /locus_tag="PC545_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2455790.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFAKKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSI"
     gene            20035..20262
                     /locus_tag="PC545_001002"
     CDS             20035..20262
                     /locus_tag="PC545_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2455791.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(20481..21224)
                     /locus_tag="PC545_001003"
     CDS             complement(20481..21224)
                     /locus_tag="PC545_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2455792.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEREYNNATSQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILNLMGFANNYGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDNGIIIYFGVDEIAPESFGIPKFELKFSD
                     FGNFINLRFYCISPDIYVQSRGRAKHFMG"
     gene            21392..22264
                     /locus_tag="PC545_001004"
     CDS             21392..22264
                     /locus_tag="PC545_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2455793.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGSYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGDIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            22264..22929
                     /locus_tag="PC545_001005"
     CDS             22264..22929
                     /locus_tag="PC545_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2455794.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKSHKDAKEAELNYNVLDFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEICHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            22950..23807
                     /locus_tag="PC545_001006"
     CDS             22950..23807
                     /locus_tag="PC545_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2455795.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIDLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSTKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKHITEISSGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(23963..26509)
                     /locus_tag="PC545_001007"
     CDS             complement(23963..26509)
                     /locus_tag="PC545_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2455796.1"
                     /translation="MNIFNKVALQGLKKNRTRTLVTIIGVVLSAAMITAVTTFGVSLM
                     NYMTNGAIIKYGDWHVQFLDVDSSFVQEQRVNDEVANTATFENIGYATLEGNRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSSKVATDGGVKFAVGDTISLDVGKR
                     MSENEKLGQSDPYTAGSEVFVPQDKKTYTVVGICRKPVFEESSAPGYTLITKEDKQVK
                     TDSLSLFVTLKNPHQIRSYISSTKDHASIFNDNVLRFIGVSDNNLFNMFLYSVGGIVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGAIGIPIG
                     VAIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIIVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQRVYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVADLKQMSNQRSEVTDQDIIFSTQGMKDNEMLKLYDKLKTANGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYEGSHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPAEEYTGQNAKLIAVSMVTKDNNIIDIFNSSSMNVSLTPETNGKLEIPERQNVNIS
                     FVKVVPPDSPPGIRSSDVKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     AEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWFSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(26509..27195)
                     /locus_tag="PC545_001008"
     CDS             complement(26509..27195)
                     /locus_tag="PC545_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455797.1"
                     /translation="MAFLKIEDLCKVYGKNENKVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(27278..28267)
                     /locus_tag="PC545_001009"
     CDS             complement(27278..28267)
                     /locus_tag="PC545_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455798.1"
                     /translation="MFRNREFRKFAILFLLITILTVALGFAISIMTGILSIVSATTFG
                     IAFFVFTKNRYKSIAQISEQINLVLHNANHLYIAESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIR
                     MDWLITSLLKLSRLDAGIVVFQKEQIDVNNLISSALHQLLIPMELHNITLHIDIPKGV
                     RILGDLNWLSEAIQNIFKNCMESVGDNGKIDIICEDNFLFTQLTIHDNGAGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAQNHPQGGAIFVIRFPK"
     gene            complement(28254..28940)
                     /locus_tag="PC545_001010"
     CDS             complement(28254..28940)
                     /locus_tag="PC545_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455799.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKEKQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            complement(29440..32646)
                     /gene="carB"
                     /locus_tag="PC545_001011"
     CDS             complement(29440..32646)
                     /gene="carB"
                     /locus_tag="PC545_001011"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2455800.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKEKPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIADTEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMGIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFRDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYSTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTAKSLRENGIEADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDKLKVYNIAE"
     gene            complement(32839..33888)
                     /gene="carA"
                     /locus_tag="PC545_001012"
     CDS             complement(32839..33888)
                     /gene="carA"
                     /locus_tag="PC545_001012"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2455801.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESVGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITIFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(34145..37351)
                     /gene="carB"
                     /locus_tag="PC545_001013"
     CDS             complement(34145..37351)
                     /gene="carB"
                     /locus_tag="PC545_001013"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDF2455802.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEVADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIADNEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVANELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGVPIV
                     DLATKCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLGPEMKSTGEVL
                     GVGENLEEALYKGFLAAGRHMSDERGVVLATVNNHDKDEFIEIAKDMKELGYTFVATE
                     GTANSLRENGIEADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRT
                     AIEFSTEIMTSLDTLKALVEVKKKHLNKDELKVYNIAE"
     gene            complement(37404..38447)
                     /gene="carA"
                     /locus_tag="PC545_001014"
     CDS             complement(37404..38447)
                     /gene="carA"
                     /locus_tag="PC545_001014"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDF2455803.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKEAIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     IENIKGTGAKVVVIDFGVKNSVLESLKACNCDITIVPAMTTVEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(38473..39189)
                     /gene="pyrF"
                     /locus_tag="PC545_001015"
     CDS             complement(38473..39189)
                     /gene="pyrF"
                     /locus_tag="PC545_001015"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDF2455804.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDRLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVKAAVRDNVWLMTIHVSDMEGMRQCAL
                     IAREEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKKGAHYLVVGRPI
                     TKAENPRESAINIVREIENA"
     gene            complement(39565..40200)
                     /locus_tag="PC545_001016"
     CDS             complement(39565..40200)
                     /locus_tag="PC545_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HDF2455805.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     RKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKIFEHH
                     GFEVFSVICKNGAMPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEAKVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(40224..41138)
                     /locus_tag="PC545_001017"
     CDS             complement(40224..41138)
                     /locus_tag="PC545_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455806.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKNFEEDEEDDLDYLAGIKTEEDDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKRPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEETQEENVTLEKEESQDE"
     gene            41739..42968
                     /locus_tag="PC545_001018"
     CDS             41739..42968
                     /locus_tag="PC545_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2455807.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLTDKCKWNIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNENLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPKGHVWLSGASKDPNGISFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDEHGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGNWAF"
     gene            complement(43173..45554)
                     /locus_tag="PC545_001019"
     CDS             complement(43173..45554)
                     /locus_tag="PC545_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDF2455808.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGLQNPDKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSKYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVQSPTVVGERLNPTGRKSLQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLESILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVNRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAITMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNEYSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKSDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDEI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(45526..46251)
                     /locus_tag="PC545_001020"
     CDS             complement(45526..46251)
                     /locus_tag="PC545_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HDF2455809.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKYTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNVVELEGTNLTLESKYVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSNLTKGLIIDSCGTTAIEEVCDIVQNNLEKVFLKQDKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISNTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(46241..46696)
                     /locus_tag="PC545_001021"
     CDS             complement(46241..46696)
                     /locus_tag="PC545_001021"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDF2455810.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(46978..48159)
                     /locus_tag="PC545_001022"
     CDS             complement(46978..48159)
                     /locus_tag="PC545_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2455811.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLILTSMYIIKSLYVNSDM
                     SVVNQLVYQARDFRSLIGYDLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDKEKDDIDELAHKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVVSQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGHYVDIVTEKKEDMAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHDGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(48149..48841)
                     /locus_tag="PC545_001023"
     CDS             complement(48149..48841)
                     /locus_tag="PC545_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455812.1"
                     /translation="MNNTVLVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMILDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIKVGVVIIDKEKHSVTVAGNEIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(48975..49691)
                     /locus_tag="PC545_001024"
     CDS             complement(48975..49691)
                     /locus_tag="PC545_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2455813.1"
                     /translation="MKKFKLIGAIILVVFISVFISKDRLLKKDKDPIVAAQHDSEQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            49816..50550
                     /locus_tag="PC545_001025"
     CDS             49816..50550
                     /locus_tag="PC545_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2455814.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRQEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMVYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT        15984 a         9260 c         5682 g        19704 t
ORIGIN      
        1 atttatataa ttttacattt tatttaaata attttaaaat aataaaaaaa ctgaaagtat
       61 caattaaatt aatacatgca aatataattt gcttattaat ttaatattat actttcagtt
      121 ttagtattta atccacatca gataataatc ttaaaatgac caaggtctta aaactattta
      181 tctttatcag atttatcttc taaattttta tttatatcaa taacattgtt tctttcttct
      241 tcatcttttt ctaattttat agttaattgt tcttcaactt ctttagaaga attttcctct
      301 gtaactgcat tttcaatttc attcagaggt aactctttat taaatgcttt tacaaattgt
      361 tctgcatcta aagtttcata tataagtaaa gcttgtgcta catactctaa tctatccata
      421 ttttcttgta atatacttct tgtcttatca tatgcaccat caactatacg ctttgtttct
      481 tggtctatct caaaagcaac ttcttcagaa taatttctct tacttgaaaa actattacct
      541 aggaatactt catcatcact gtcaaatgac attggaccaa gtttagaact cattccatac
      601 tttgttacca tacttcttgc agtagctgta actctttcta agtcatttga agcacctgta
      661 gatacatcct taagtactag ctcttctgct actctaccac caagtaaaac tacaatattt
      721 tctttcattt cattttttgt agcatagaac ttatcttcaa caggaagttg cattgtaaac
      781 cctccagctc ttcctcttgg aactattgta acttgatgaa caggacttac ttcttcaagt
      841 acatgggcac aaactgcatg ccctccttca tggtatgctg taagtcttct ttctttctca
      901 cttattactt tcgatttttt agccacacct gctataacct ttgttatagc ttcttctata
      961 gtttccatct taattttctt ttctcttttt ctagctgtta atattgcagc ctcattcatt
     1021 aagttctcta tatcagcagg agtaaatcct ggtgttcttc tagccaagac atccattttt
     1081 acatcatcac ttaatggttt atttcttgag tgaactttaa atatagcctc tctacctttt
     1141 acatctggtg taccaacaac aacttgtctg tcaaatcttc ctggtctaag taaagctggg
     1201 tcaagtatat ctggtctatt tgtagctgcc atgattatta taccttgatt taccccaaat
     1261 ccatccattt caactagaag ttgattaaga gtttgctctc tttcatcatg acctccacca
     1321 agacctgcac ctctttttct accaactgca tcaatttcat ctatgaatat gatagctgga
     1381 gcacttttct tagcctgttc aaataaatct cttactcttg aagcaccaac cccaacaaac
     1441 atctcaacga aatcagaacc acttatactg aaaaagggaa ctcctgcttc tcctgcaact
     1501 gctcttgata gatatgtttt accagttcca ggaggtccta ccataagcat tccttttggt
     1561 attcttgcac ccaattcaat atatttctta ggattcttta agaaatctac tacctcctgc
     1621 aaatcttctt tttcctcatc tagtccagct acatctttaa atgtaactct agttttttca
     1681 tcatctttat gaaccttagc ttttgatttg ccaaaactca taacctttcc gcctccacct
     1741 tgagactgat tcataaacac gaaccaaata atcaccatga agaatattaa cagtaatgta
     1801 ggcaacattt cgacaaacca aggtgtagat ggcttagctt caccaccaaa agttagtttc
     1861 ccagccttag cttggtctaa cacctcatct gcaagtttat ttcccatcac ttctgttgga
     1921 acatatgact taaactttgt atttgtatct tttatagtac cttctacaga agtttgattt
     1981 acaaagtata atcttgatat attctcatct gttaattctc tatatacttc agagaatttt
     2041 aaatctttta ttttttctgt aggcttacct gagaattgaa ctatacctac tataattata
     2101 aaaacaagta gataaaaacc tgcccctttt agcaacttat tcaatataga agccctcctt
     2161 tcatacattg ctttgcccta taatttgtta aaattaattt ttaatacttc ttttgtgttt
     2221 tcatcaattt tataattttc actgatttta taatctccaa cacatattat tccttcacta
     2281 tctgtgatta taggaatttt acatctttct tctcttggga tttttaaatc tatgaataaa
     2341 tctttcactt ttttacttcc catagcaagc tttatcttat caccttgcct tctgcttctt
     2401 attactatac ccccttttac cttattaaaa tcaaaccctt tagatgaatt gtctaatttc
     2461 atactcttat acctatctat gctcattact tgggtctcaa taatttgttt taattctttt
     2521 attttaataa acccattact aggtacattg taataaaatt caatttcctc attaacaatt
     2581 tcctcattcg ttaatattat actgtctttt tttctataaa caaatatatt tcttggaaga
     2641 gttaacattt tatttacttt tgaattatct tctaaagaca ttatatcttc tatatgtctt
     2701 tggtcaacaa aattagtatc tcctagtata tgttttatgg aatttctaag aactctaact
     2761 ttgatagcat catgtaagca aacaaaatcg tctaaattta tctctacaaa gcctttttct
     2821 tttatatttg aaacttctct aaatttagct tctgcctctt tctcaatata atcgttatca
     2881 ctttttaaac tattgctcat cctcactata gattctatta catttgaatt aaaattatcc
     2941 ttcatatatg gtaaaaggtc taaccttatc ttatttctag tgtatatatt ttctaaattt
     3001 gttttatcta tcctcggatt taaattataa gcttcacaat actcctctat ctcatttctt
     3061 tccacgtcta atatgggtct aattatgcaa ttatctctaa tataatcaat tccctttaag
     3121 ccttttaaac ctgttcctct catgatacgc atcataaccg tttcggcttg gtcattgaga
     3181 ttatgaccta ttgctatttt attggcttta atcttatcct taatttcata aaacatttca
     3241 tatcttagct tccttgctcc ttcttctaat gataatccct cattttcaca atattttggt
     3301 acattaatag actttacaaa gaatataatt cccatatcct cacaaagttt agatacatat
     3361 aatgcatctt tttgagcttc tatccctctt atttgatggt ttaaatgtgc tgcatatatt
     3421 tctatattaa aatctttttt taatctattt agtacatgca ataaacaaac tgaatcaggt
     3481 cctccagaaa gacctaaaac tattttatcg cctttttgta ttaaattatg tttatttata
     3541 gtacttagta ccttatcaaa tatcatattt atccctctta cacttagttt tattcacttt
     3601 taagtatact ttctataaaa aaacgctaaa cccttgtatc aattttaaga aattaaagtt
     3661 taatataaat ataataatac caaataaatt aaataaaagg tagttttttc actaccttta
     3721 taaaatttct ggaaaatagc catagttatt atttagtacc tatgtatata tctattttat
     3781 tcctacctaa aaataaatct tgtaccttta tattatagtc tattttcaat tttatgtgtt
     3841 ccccttcttg tatatttata tctaattctc ttgtatcaat atcaattaat aaagtacctt
     3901 gtggggtcac atactttgta gtactacttt ctccacattt aaacagcatt gttgagttaa
     3961 tagcaccaaa ctttttgata ctaacttgat ttttaagtat ctttatagta ttagttattt
     4021 ctatatcttc ttctttttcc ttgtaaataa catatattcc atcattttta taaaaaattt
     4081 taccaaaggc agtcatctct atggtgtcca tatttccttt ttcatcatat tgtctagtat
     4141 ttatacttat ttttacactc aacgattttt cctccataga ttcttcatat aaactagtat
     4201 ttttgtattt ctactttttg aatcttatca atttttttct ttaagaactc tcttgctata
     4261 tctgaaaatt tttcaggtat atctgataca taatattgat aagttccatg aatttctgta
     4321 ttgtttataa tattttgcac ctctaaaatc ttttttaaat cctttgcagt ctctttagct
     4381 ggattaacaa gttttatatg ctctccaact tcttcaccta ttgttctctt taaaatagga
     4441 taatgagtac atcctaacac taaagaatct atattttttt ctttcaattc ttgtaaatat
     4501 atctttgctg tcagtttagc cacttctgta tttgcccatc cttcttcaac aataggcaca
     4561 aataaaggac atgctttatt aacaatttct atactctcat ctattttaga tatttccaag
     4621 ttgtatgctt ttgaacttat agttccttca gttcctatta ttcctacaat tttgtttttt
     4681 gtagaactta cagctgttct agctcctgct tctataactc caattatcgg tatatcatat
     4741 ttttcttgag cttcttttaa gcttcttgca gtagcagtgt tacatgctat aactatagct
     4801 tttacacctt ttgtttttaa aaagtttata gcttgaaaag tatatttaat tatagtttct
     4861 ttggatcttg aaccatatgg tattctggca gtatctccaa agtatattat atcctcattt
     4921 ggtaatatct tcattatctc ttttagaact gttaatccac ctaatcccga atcaaaaact
     4981 cctattggtt tattgttcat ctttatacaa cacctacttt acatttttgt gtttaacaac
     5041 aacttattta tcgctaaaat ttatatttat attcataatt aatatattat tacctaaatg
     5101 attattatag tttattttac acaaaaaata aaggagctat cttaaattag aaaataattt
     5161 ctacaaaaag acaactcctt tcactactta aaaatttaaa tttattttgc aaacacatga
     5221 tttcctattc tcatataagg attcaatcta tttatccatt tgcaagtggc tatttctgga
     5281 ttccaaaaat atactgcttt attagttggg tcagttccat aaagagcttc ctgagctgct
     5341 ttacgtgctg agtctgttgg tgccatcttt attgaaccat caagcacaac tgagaaagca
     5401 tttttttgat atatgacttc ttctattgtg tctggaaatc tagggtccaa tactctattt
     5461 attactacag ctgctacagc aacctgtccc tcatagcttt ctcctctagc ttctcctgca
     5521 accaacttag ataataacat caagtcatta tcagataaat ttattacttc tttctttgct
     5581 atattttttt cttgtttgtt tactttctta atattttctg ttggttcata tgcaaatacc
     5641 tctttaatag gcataaatat aaaagtcaaa gctaatagtg tagccatcag tttactccac
     5701 ctgtccatga ttaaaaccac tccttacatt cttattttta aaattttttc tctaaatcct
     5761 tgactacttc aagtacttta aatttaattt ttactctact tgtttttttt ggtgtatctt
     5821 gttttattaa cttatattct ttatcagtta aaacttcctt caacttatca tctgtagatt
     5881 tatctatcac tccattagat tcatcaacaa aacatagtgg agggaacata acacaccacc
     5941 agttttttcc ttcacctttc ccaatgataa tctttaatgc tttatattca ccagctggaa
     6001 gaactatatt agaatattgt ttagtaggga aattgctata ttgtatacct acctttacac
     6061 tgtaattata tccattttta agaataatat ttttacttat ttcttccaaa ctactatatt
     6121 ccttttttat aatagcttca ctttctttta tactttttga attttgaagc tttggctgta
     6181 aatattttat tacttcatct ctaactttta gtttaagttc ttggtcttca tctgtattgc
     6241 tgtttgctat aacatgaaat cttattaatt tatctttata atcttttgat atattgtcta
     6301 ctttttttac ctcaccattt attactattg tcattattga tatgactgat ataagactta
     6361 atattaatag tcccaatcta atcttaattt ttctcattga aaattccccc tcaagataaa
     6421 tttatatcat caattatgtc caattaaatt taatttattc ataatatttt ttaatactaa
     6481 taaaaaaagc tatcttaaaa tgattacaat cattttaaaa tagcttttta aaccttatat
     6541 tttataagtc tttactaatc atagccttat ctatcataaa ctgctctgct ttttctatat
     6601 gctctgtagc atacttcttg gatagctcac tatcaccttt aattatagca tcaagtaaat
     6661 atttatgctc ttcaacaata ttatgtgatg tatcataagc agacatatag gtaactctaa
     6721 atctatatac ttgttcccac aatgagttta ttaattggat taatttttta ttattagttg
     6781 ctttaaatat acattcatga aattctacat caagttttaa taacgcatct acatcggatt
     6841 ccaaagtgct ttttttaaat tcttctgata tatctttaag ctttttaata tcttcatcac
     6901 taattctttc agcggcaaga tatgctgcta agccttctag gcttgctctt acttctaata
     6961 catccataat atcctttaat gacatatttg ccacatacgc accttttcta ggcaacataa
     7021 cgactaaacc ttctaattca agctttctta ttgcctctct cactggagtc ctacttactc
     7081 ctaattgttc tgctagttgc acttccatta gcctttgacc aggctttaat tttccttcta
     7141 agattgcttc tcttaaattc tcaaagacta catctcttaa tggcttgtaa ttatccaagt
     7201 tcagtttagt taaattctcc acaaatttca accccttttt gactgctatt aacaacatac
     7261 acctgtttat atttttttaa taattctttt tttccaatta gagcatcttc tttattttta
     7321 aatagtccaa aaactgtagg accacttcca ctcatcatag aacctaaagc attatttttc
     7381 atcattactt gttttatatc acttatttct ttgtgtttac ctatagtaac attttcaaga
     7441 atatttacca tgctttctga tacagctttt atatcttcag attttaaaca ctctatcaaa
     7501 tatttattat ttggtctttt tttaatatta ttcaaatcta atccttgata aacttcttta
     7561 gtagaaacga ataagtctgg tttgcatatc aatatatttg tatcacatgg taaacctttg
     7621 atgtttgtta gcttttctcc aataccttga gctaaggcag gtcttcctga tatacaaaaa
     7681 ggaacatctg cgcccaaatt aagtcctatt tcttttaatt catcttcaga taatctaagt
     7741 tcccataggt gatttaaacc tactaatact gcggctgcat tagaacttcc tccagccatc
     7801 cctgctgcta caggaatatt tttttctatg aatatttcta cacctttttt tatataaaat
     7861 ttatttttaa gtattttggc agctttataa acaatattat cttcatctaa aggaatatct
     7921 aaactagtgc tttttacctt tatttcatct tcgtctaatt cttttatctt tactatatcg
     7981 tataaatcta tcgtttgcat tatcatttca acaaaatgat aaccgtcttg cctttttccc
     8041 aagacatcaa tggataaatt tatttttgct ctactcttta gtcttataaa atccattcta
     8101 tcctccttgt ttaaagaata atgcatcact aacaataata ttatactaca aaaaataaaa
     8161 taatagggta ttataatacc ctattatact taattacttt aaatttttta atattaatct
     8221 actaatacga cttttttttc taaaataaga cacttaccag gcttaattgg ttcatcaagt
     8281 tttatatcat taagttctgc aatttcattt tctgttgtat tgtatttttt tgctatattc
     8341 cagaaagtat ctccttcttt gcatatatat acgattatgc taggagcttt agataaatca
     8401 tatacacctt ggtcttctcc cttgataata aaattttctg ctttcttatc taatgcttca
     8461 gtaaatctct taattttgat aatcaagtct atctggtccc tattcaaatc aacttcgact
     8521 ttatcaatac atgctgtatt aaacacagat gctgtatcag ttagattatc catttctata
     8581 tcatgttcaa atggaatttc ctcactaatt ttataaacca attttaaacc ttcaactggg
     8641 acaaataaaa tttcaacttt tatgattcct tgtataatgc ttttatttcc ttcaatataa
     8701 ctattttcta tagacatggt tggacaaacg cttacaatat cttttatttg tatatcatca
     8761 ttatcatttc ttatgccttc tctaaccata aatgtttctt cactacgtct taaagtctta
     8821 tttaattgaa taggtttgtg gtcaaatttt attatctttt gaggcgaata tgcatcttgt
     8881 aatacttctc ttgtaacttc atctgtaact tttaccttac aacaagccat acaatctatt
     8941 tctaaaagac ctgtgttgct ttcactattt tgcttgaaaa tatgattaaa atctgacata
     9001 gacaacaata cttcttcagt cataccatca ctaactccag ggacttctac aaattgagta
     9061 aactcaatac ctactctatc caactctaca agttcaccct catatgtaca agctaacgga
     9121 tttatttcta atactccacc aataataact ttattgtcag tcactctact ttcttttact
     9181 ttaacacatg gatttaaact tattatagat tgaatttcct ctgtgtttat tgttatagta
     9241 tctcttatag aactttctgc tttttctatt ccaactatat cttgaaaaca aatttccttt
     9301 ctgtgctttt gtatgccttc cacttgcgcc acatctttta caatgtctaa cctttgcttt
     9361 tcaaacaaac ttccccttat attcataaga gctcctactt taatctttct ctcattcatt
     9421 atagtacaat ccatatgctc tacttctgaa aataacatgt attccatatc ttgaacaaca
     9481 ttgtcttttt ctattacttc gtttatatct acttttccgt ctacattaga aatagtattt
     9541 ttatcatctg caatatagat tacattataa ttaaaactac ctctgcaaag tatcttccca
     9601 tctgccattt caattttttt aagtgatatg tatccttctg tttttacaat ttcatataca
     9661 tctaattttt tgtctggtac aactgcctcc gcttcaatga atgtttgaaa ttttccaaag
     9721 tctattctat tgtcaacttt aattacatct ttaattaatt ccatagtcta cctccttaag
     9781 tattataaaa atatcacact atatttatat ttaaggagca aaaaaaaaat tacacctatt
     9841 tttaaatagg tataatttta acttatttgt aatttatctt gatctctaaa aacttgcaac
     9901 tttacattag atgttaataa atctgagtaa ctataagata ctctaggata tccattactt
     9961 tcatcatcta actttataac aaacacgctt ggataaactt tttctagtat accctctttt
    10021 gtaataatct gctttcttcc tttattagct ttcaacaata ttttctttcc tatatgtctc
    10081 tctaagctta ctcttatctt atctagagtt tgaacagtag ccacaatatc accttcttta
    10141 acgtttatat gtttacataa tatcacatat aaacattttt gtcaaatgtt taaactaatt
    10201 attttatata ttttttgcaa aaatgtcaat actatttttg aagatataca gtataattat
    10261 gttaattttt gctaatgtaa tattgttttt ggcattgccc atctaaattt tcctctggtt
    10321 tcagaaccac caattccctt aacacctgta atagtctgct ctataacctc atcaatagga
    10381 actactacat caattcttga acaagcaatt ttctccacta taaaaacagg gtctaatgca
    10441 tgtatcatta tccttccagg cgaacttgca tagtttgctc ctgcacttat tattttttca
    10501 taatttgatt ggcatgctcc tgcgaatatg acaagattat ccaaatttgg ttgccattta
    10561 cgtgcttctt ttactgtttt aacaaaattt gaagagtttc tatagttact catatcttct
    10621 atatctcctc tttttattgt cattgcatca tgacctgtta ttactagtat atccggatta
    10681 tatttttcta gtagtgctct tacttcttta tactgatttt gctctgaaac agctacacct
    10741 actgcaggta ttccaagttg agcatataca tccaagcaaa tttttaaata ttctttatct
    10801 ccatctatct gtagtacttt tccaggcatt ccatatgtgt taggatttaa ttgaagtttt
    10861 ggaactgctc tagtcattct tttttgtctt tctttagctt ttcttactga cttatataat
    10921 aaattttcta catttttatc tattaatata tcttttatat caggtgcttt tactaattct
    10981 aggtcatcaa tataagcatc tgctattact ctaaatgcaa ttccctttag tatagctatt
    11041 ttctcattat tttcatctac accaaaagag actattttaa atactatatc tttgttatgt
    11101 gactttctag ctacaatgtc tcctaccttc atagttaaac ctccaataaa tttttatctt
    11161 agtttatact atgttcgata taaaactttg tttcctaatg tcaaaaaaac atttcttata
    11221 atacttcttt tatcactctt aaagcatttt tatagtatat cttttctata tcatcttcac
    11281 taaacccttc tttttttaga ggttcataca gtttacccat ctgagatata tcttcaatct
    11341 caacttttga atctattcca tcaaaatctg accctagaga tacaacatct attcctccaa
    11401 catttacaat atgttttatg tgtctaacca tatcttcaag tttactttca cttttatcac
    11461 ttaaaaataa gtggaagaag tttatacctg taactccacc tttatttgct aatactttaa
    11521 tcatatcatc agttaaattt ctagaatgat tcatcattgc tcttgaatta gaatgtgtag
    11581 caattattgg tttagaagaa atctttgcaa tttcataaaa tccaccatct gaaatatgag
    11641 aaacatcaac caacattccc aattcattca tcttatgtac cacttctctt ccaaaatcag
    11701 taagcccttt ttctctatat tctactttat tatgaggaaa gctcaattca ttcacatgat
    11761 tccaacttat tgtcatcatt ctaaccccta agtcgtaaaa tttctttaaa ttttctaatt
    11821 taccttcaag tactgcacct tcctcaatag ataaaagagc tgtaagttta ccttctgatt
    11881 ggtttttcat aatttcttca tagttagtag ctaaagctat ttcatcacta ttttttttca
    11941 tttcctcatg aaatttattt gccatactca tgcaataatc aaaaggatgt tttacttctt
    12001 cagtatcaac aaaaagagca aatgtttgag ctaatgaatc cccctttttc aatctgtcta
    12061 tatcaacaga atatttatta gacttaagtt cacttgtttc tacattttcc attaattttg
    12121 ctatagtatc acaatgtaaa tcaataaatt tcatatttat accccctgta tattttatat
    12181 ttatatctaa aaatagaaat agttgtcctt atagacaact attgacaact attttttaat
    12241 attcttttta atattactta atcttattct tcttttaagt taggtattac ttttttaact
    12301 tcttcaattt gatgccatgt tttatctata ttattttcac taatatattt ttttaaatca
    12361 gttaaagatg taaaatcttt aactgataaa ttgtcatcat catctgcaaa tcttaaaata
    12421 aacatgagac tcccttactt tccacaacat ttcttatatt tttttccgct tccacatggg
    12481 catgaatcat ttcttcctat ttttgcttct ttaactattg ttttagactt cttataagcc
    12541 tttattattt cttttctttg gtctgctggt agtatatctt cccaaccttg taaattatat
    12601 agccaatgag cctcaacagt taccatattg ttatataatt tttcccaatc tatttttata
    12661 gaaacttctt tatcagcttc tacttcttct aaaactatat cttcttttaa gctttcactt
    12721 attccatcta tgaatcccat gaaaaattca ttagtagttc caaatttttc agccaattca
    12781 cttactttac cttcaacaac atccatttta ttatttaata tttcattgta tattccagct
    12841 tcaactttca aatattcttc ccagaatttt acttctgctt cttgactttc atgattatca
    12901 cttaaatttc tccattcagt atataaactc atactttttc ctcctaagta tacctatatt
    12961 tttatagttt ttatagtata aaatctttta gttagattgg aagtttcttc tttttgatta
    13021 taccatattc tatttaaagt taactcttat attatatcat aattaaacaa ttcttttaat
    13081 acttttatag catactcttg gtttataaaa taagggcttg tcgcaattgt tattactttg
    13141 gcttttttaa ttaagttttt tatcttgtaa aatttgagtt caaatggtat atagtccata
    13201 tcacttgaaa atatatccaa atctatgtca agaacaaact ctgattctac ttctaaatca
    13261 aatccatata gactatctat tattattaat tctgaaaata tgttatattt taaagcaggt
    13321 tttataaaac ttcccacatt taaaacttca tttgtatatc taaatacatc ttttagatta
    13381 tttacatcta catcatagtt ctcaggttct ctagtatcct tatgttggtc tacatgcact
    13441 aacttgcatc cttttgtaaa ctcacccttt aacaaagact ttgcccaaaa ataaaatgca
    13501 tggttatgat tgtcaaacac atatatcttt ttatttccca ggacatattc gaccatattc
    13561 tccaatcctt ttgcttttat ttccttattt tcatctactt cattaaatac tacctcattg
    13621 cctaacctta catcatttaa tgtaccattt ataagttttg gaacgtatat gctcttattt
    13681 tctcttttat catatgaaaa tacattattt cctactggtt tttctatata aaatccacta
    13741 tattcataat tttccataaa actttatttc ctccattgtt atataaatat ataagttata
    13801 attttaaaaa ttaaaatgca cttattgtaa atcatatcat ctattataga aaacatctta
    13861 tataactaaa tataatatct tctctcaata tgtattatac aataagtgta aaatatattt
    13921 atataaaata tactacattc ctataacaaa accatcagct tcaattggaa gataactaac
    13981 tcttctattc aactccacct ttccaaaaaa tactacccaa tatggtctaa aaatactttc
    14041 aattttaact atttcaatct ctgggattcc tcccataaac ctatgagtta atctcatagc
    14101 cattttattt gcctttgctt ttactcttcg ttcgtcctta tcactatact gcacaatatt
    14161 acaatccaca ttttttacaa cttcatatgt aggaaaatct tgtactatac taccagaacc
    14221 tgtagaacca ttcactaaaa tttttaattc atttatatca ttagaatatc tcctattctt
    14281 gaaaatggat ttcgttttgt gaattttaag tgtgataatt ttaaactcta tataatgaag
    14341 tctaacttca tgagcatatt tgttctttaa tattagctta ctaaataaag aattttttcc
    14401 acttttagct atctctatag cttcttcttt tgtaattctt ataggtaacg ctcttatatc
    14461 cataaaatca ttctctttct tatttttttc ttgtcatttt taaaactcta tatatataat
    14521 caaaaaatat tacaattaca actgacataa ctccatatgg aattattttt ccaactataa
    14581 tatcatttct agtcaatata ccataaaata taaatcctgt tattaaaaag tacaataata
    14641 ctagaaaacc gtctattcca agccatttta tttctaaatc tttttttacc ttagacacta
    14701 atttagacat agcagtttct ccttttatag attattttgt gtatttaaat tttcacctat
    14761 tctattacca atcatccatg caataaatga aaaaggtata gctatcacaa gaggctctaa
    14821 ctcaaagctc tgaactggaa atattttaaa ataaccaaaa gtcactccac caacaatcat
    14881 tcctaaaata ccacccatag atgttcttct tccatttcca agaagcccac ctactatagg
    14941 tacaaatgcc gaacccatcc aaatacttcc tacaaatata catgcatcat atacattttc
    15001 aaatctaaat gctacgctaa gccccataac tgctgctaaa catacaccta ttctagtaaa
    15061 ctttactaat tgtttctgag ataactcttt ttttgtaatt cttccatata tatcatttgc
    15121 taatgtagcc ccagctgcta gataatagct atccaatgtt gatattatag aacctaaaat
    15181 tcctattata aatattactc ttattccttg tggtaatgta tctaaaacta aagttatata
    15241 tccagcagct ggagccattt ctggataagt agctcttact ataagtcctg tcatagttaa
    15301 caccatgtca aaacatatcc atagtgaaaa acaggttaat aaggctcttc ttccaacctt
    15361 aggagaatct gctgctgaaa atctttgata aaaagttggg tctgaataag ctccaaaaga
    15421 atatacaatt aacatcaatg ctttcatagc tgacataccc tttgtagggt gcataagctg
    15481 tggtgcatct ttgagtgctt ctgtaagacc ttgaaaccct cctatttgtt caaattgacc
    15541 tggcataacc atagttaaac caaataccat acaaaaaaac aataccatat ctgtaatggc
    15601 aactcccata agcccaccaa gtactgtaag tacaacgact actcctacca ttattaatcc
    15661 tattaattct ttatctattt tccatgctac gttacctata gttcccatag cagttatttc
    15721 tccaagttgt gcacaatata taaacatcaa aattgcccca attatagcaa catgcttgcc
    15781 ataaaaactt tccagtaaat caggaactgt tatattggtt cttatatgaa tccttggacc
    15841 aacccacaat gctaaaggca ttctcccaat atgtgcagct attgaccaaa ctgcccatgc
    15901 agatattcca tatatagccg catactctac actacctact gtaccaactc ctccatacca
    15961 tgatgcaacc aaagttccta ctattaagga ccatggtaaa cttcgatttc ctaggtaata
    16021 actttccata tcttttacat ttctagaaaa ataataacct attcctaata atataaatgc
    16081 aaatataact ataatcccat tttcccaaaa tgttaacata tctaatctcc acattcttat
    16141 attttaattt ttaactttaa tatctaaata tgaacaactc tacccatttc cataaaaata
    16201 ggtagagtta tataataatt ataagtattt aagtaatctt gttattaagc aattttattg
    16261 tttaaacctt tattttctct tttatttatt ttatttaaag cataagtagc aaatatccct
    16321 ataactgcac atactacaga taaaataaat atatatgtat atcctgtttg tccatattta
    16381 tctaaccaat tacctgcaat agtgtaataa aacgcatctg gagaaaatcc tataaatgaa
    16441 gcaaatccaa ctactgctcc tgtagtttct aaagatatat tagactctga aacagatgca
    16501 aagtatatac ctctaaatcc aaataaaatt acacttagta ttatcatgtt tattacagct
    16561 atccatataa atcctgtact cttaggaata accaagtaag cagctgtact tactgtcatt
    16621 aatataaatc ctacaaacat tactcttata gatgaaccaa ctttatctgt tataacacca
    16681 gcaactggag atgctcccat ttttataaca taagttctta ttattgataa agcagaaact
    16741 agagtcattg gcattacata aacttctgat aagtatggac ttaaataagt taaacttgag
    16801 aatactaaat aagttgaaaa tattataaga ccaattagcc aagcctttgg cattgtaact
    16861 acttttaaca atgacttaat acttaatcct tcatctgatg ctccactatc ttttttctct
    16921 tccactaaga aaaatagaag tataccacat attatagatg ctgcagaata taaccatact
    16981 acatatttga atcctactgt tatttcagca aatttaccaa agaagtaaag acctataaaa
    17041 gaaattaatg tacctgatac acctgataac ccttcataaa aaccaaatat tcttccttgc
    17101 tctgactcac ttccttgcat tctaacaacc ttaacagagg ctgagaagaa agttaatatt
    17161 gttgtaaatc cccataatac aaatattaaa agtatcatac tatatgatgg gaatgtagag
    17221 aaataaaatc caagtattcc tgaagcaatt aaagaaaaag ttattaattt tctaactgag
    17281 aatctatctg caaaccatcc tcctataaaa taagatacca ttcctactaa tccatataaa
    17341 ctcaataagt tccctatttg tgtatgactt aatgctaaag cttgttgcat tgggtcataa
    17401 aatgatgatt tcatatatgg taaactatac atagctgttg ttccaaatgc caatatgaat
    17461 attatcaaaa atcttttaac attatttttg ctttccattg attctctctc cttaaaattt
    17521 tattgtttat aatctcattc atataatttt attttaattt ttctattgct tgtttaaaat
    17581 catttattat atcatttgaa gattctaatc ctacagctat tcttattaga ttttccccat
    17641 tcatttgaac taatgtatgc tcttccccta agcttgttgc tattgttgca agctctaaag
    17701 aattcattaa tcttctacta gcttggtctc ttgtcaatcc attgatacca tccttaagtt
    17761 taaatgaaac tacacctcca cctaaaccat tcatttgagt tttacaaagc tcatattgag
    17821 gatgagattt taatgctgga tactttacca attccacatt ctcttcttcc tctaaaaatt
    17881 ctgctactgc taatccattt tcacaatgct ttttcatcct catatctaac gttttcatac
    17941 cccttaaaat catccatgca ctcattggtg gtaaagatgc tcctgtaaaa caacataaac
    18001 ttggccatct aactaaatct attaattctt ttgaacctgc tactgctccc cctaaagtat
    18061 ccccatgtcc atttataaac ttagtaagac tatgtattac taaatctgca cctaaatcta
    18121 ttggtctttg tacaattggt gtcccaaaag tactatcaac tatagctaag cttttatttt
    18181 catgagcaat ttctgctatt gtttttatat ctactaagtc taatgatgga tttaaaggac
    18241 tttctatata aactatttta gtatttggtc tcattgcttc ttttacattt tgtggattta
    18301 ctgcatctac aaatgtaact tcaactccaa atttagataa caactcagac atgaataatt
    18361 tagtgtgtga aaatattcca tctgaactaa ctatatggtc accacttttt accaatgcta
    18421 atatagtaga tgttattgcc cccatccctg atgtactaga tatggcactt tctgcatttt
    18481 caagcatagc caacttagct tctaactctg taagagtagg attacactct cttgtataca
    18541 catatcccca tgtcatacac aactcttcaa aatgctctac tgtctttgca gcaaatgttg
    18601 ctgtttgaca tattggtgta gccaatgcac ctgttgttgg gtctgcacta tgtccccaat
    18661 gtattatttt tgtctctaaa tcttgattaa tattgttact cattttgatt tcctcccatt
    18721 ttgacttatt tatacaacgt tttccctacc aaatttcatc aatttagctt agtcttaata
    18781 cattttttca agtatacttt tttatttgtt ataatttcta aaaatatgac acttattttg
    18841 tttttttaca gttctaatta taaactctat agtaactata gagtcaatat gtttttgata
    18901 ttttttttac aatgtttaaa atagtcagta caaaaatagg tatatcttag aataactaaa
    18961 gtcatcctaa gatataccta tttttatatc acaatataaa taattacata taagcattag
    19021 tttttttatc ataaatagat ttttcaagat ttatcatctt ttcataaata tattatagtt
    19081 aaatatttta atattaccta acatctacaa taaaatatac tcttaaaata aaattatata
    19141 attaaaaata ctctataaaa gctaaaaagt ttttctattc gctacatcta aaaataatta
    19201 gatagaggta taaatgtatt tagtttattt atacctctag tcttttataa attatatctc
    19261 tactaaattg attttttagc acgaataagt gtgctcttgg agataccagt catcttcaca
    19321 acctctctat atggcttaga atttaataac cccaatgcat attgtatctg ttgattgctg
    19381 tatttctttg gcctaccatc tttaaagtcc tttttctttt tagcaaattc ttttcctaat
    19441 tgagtacgtt ctattatcat atttctctca agttctgcaa ctgcaagtaa tgtagtcaaa
    19501 aagaatttac ccattgaagt attttctaaa aggcctatat ttaaaatatg aacacttact
    19561 tctttagcaa ataaaccttc tattagttgt attccttctg atgtacttct agctagtcta
    19621 tccagcttag ttgcaacaag taagtctccc ttttttagag atgatataat tttttcaaat
    19681 ttaggcctgt ttgtagtagt gcctgtataa gtttccttta ttattttagc atcattatat
    19741 ttattcaaaa tttcttgctg ttgctgatac aaactgttgt tgtctgtttg attttttgta
    19801 cttacacggc aatatccata tataataatc atcacttcct aacataaaga ttttgtacag
    19861 aagttaccca gaagcttgtt tttacttatc tttatccatt ttactaaaac gatttttggg
    19921 tgtcaatact gttaactttt gacaccataa taattccttt ataatttgtt tgactatgtc
    19981 aaaaattacc aatataatga aattaatgag tttatacaaa ttggaggaaa taatatgaat
    20041 agtctgttaa aagaagcaat agatgaatta aaaaacatcc attgcgggga agttttttta
    20101 ttaaaagaat tgttcaaagg ctatgaatgg aacaggttgg agatgaatat cagattgaaa
    20161 ttaggaaaac tttttttgta tgaagtggta actaatccca atttaaatgt agaggtcatc
    20221 aataaaaatt ctgcttatca gcaaatgtat aaaaaaaaat agtgacttaa atgtatataa
    20281 tagtataatc ttcttattga ttcaaaagtt aaaactttta atacaaaaca atcattctat
    20341 tttaacattt atcaatatgt ctaaatttat ataattaaca catacgaata gtattgttta
    20401 aatcacatta tttaagatag ataaatctta accctactcc agcataaata ctaaaatagg
    20461 gcttagaatt atctatattt ttatcccata aaatgcttag ctcttcctct tgattgaaca
    20521 tatatatctg gggaaataca atagaagcgt aaatttataa aatttccaaa gtcagaaaac
    20581 ttcagttcaa acttaggtat accaaaactt tcaggtgcta tttcgtcaac gccaaaatag
    20641 atgataattc cattatctgt aagataaaat gcttggtctt caggtataac taactcaaca
    20701 ttagaataat acatttctct attttcattt atttgagatt taatgtaatc agtaattata
    20761 ttaatataat caactccatc tctaaaaatg tcattaagta aaagttcttt tccagttgtc
    20821 aaatctatat tgtaattatt caatgtattg tatttaggcc catagttatt agcaaacccc
    20881 ataaggttta atatcatact cagtacataa tttttattaa aagtaactgc gtaactggta
    20941 gatacccaat agctttgttt tggaagtgaa ttttgagaag ttgcgttatt atattctcgt
    21001 tcttcttcct gtaagccatt tttaaaatta tttacagtca ttcttacagc attgtttatg
    21061 ttatttaaaa taactgggtt tacatacgtg gattgatttc taatattgta tataacagga
    21121 tactttacat catatgaaaa atacttactt ctttcaaaat cttcattaaa ttgtacatat
    21181 aatatattat atgtacaggt atttgtagat tgataatttt ccattaaaat cctccccctt
    21241 ttataaaaaa atcagataca atataatatg cttaaaacag taataatgtt aatggtaaat
    21301 gtaaagaata gaaagtatct aaaacagcaa aaaatgacta attttgtggt ataatacata
    21361 ggattgagag ttattttata ggaggaaaaa tatgttatta ttagcagata aatggaaaga
    21421 ttatgaactt atagatatgg gaaatggaga aaaattagaa cgttggggaa gttatgtact
    21481 tagaagacca gacccacagg ttgtatggcc aatggaatct gaatgggctt tatggaaaaa
    21541 ccctcatgga cattatcaca gaagcaataa aggtggaggt caatgggaac acaaaaaaag
    21601 atatccagaa caatggacta taaattataa aaacttaaag tttcacatta aaccaactgg
    21661 ctttaaacat actggattat tcccagagca agcagctaac tgggattgga tgatagagaa
    21721 aataaagaaa gctaacagac ctataaaagt gttgaatctt tttgcatata caggtggagc
    21781 tactgtagca tgtgcatctg ctggtgcaga agtatgtcat gttgatgctg caaaaggtat
    21841 ggttaattgg gcaaaagaaa atatagaatt atcaggtctt aaaaatcaaa cagtaagatt
    21901 tatagtggat gatgttgtaa agtttgttga aagagaaata agaagaggta aaaaatatga
    21961 tgcaataata atggaccctc cttcttatgg aagaggtcct aaaggagaag tatggcaaat
    22021 agaagaaaag ttatacagtt ttgtagattt atgtatggga gttttatcag ataacccttt
    22081 atttttctta attaattctt atacaacagg attttctcca atagttcttg aaaatgtatt
    22141 gtctacaaca ataggtaaaa agacaaatac aggagatata tacggtgggg aattaggaat
    22201 accagcttcc agagatggta agatacttcc ttgtggtata tttggaagat gggagaaaaa
    22261 ataatgataa aagtcatata tgaagataac catttattag ttgtagaaaa accagttaac
    22321 atattgtctc aaggagataa tactaatgat aaagatatgg taaatctttt aaaagactat
    22381 attaaaaaaa agtataacaa acctggaaat gtatatattg gtttaataca tagattggat
    22441 agaccagtag gtggagttat ggtgtttgca aaaacatcaa aagcagcatc tagattgtct
    22501 gagcaggtaa gaagtaagac tttcaaaaaa acttatctag ctgttgttca tggcaagata
    22561 aaatctaact cagatatact aaaagatttt ttatataaaa ataaaaaaac taatatggta
    22621 actgtagtga aaaaaagcca taaagatgcc aaagaagctg aacttaacta taatgtatta
    22681 gattttaaag aaaatctaag tttagttcaa gttaatttaa agactggtcg ttctcatcaa
    22741 ataagagttc aatttgcaag cagaaaacat ccattatacg gagaccaaag atatggcaaa
    22801 gaaataaata agattggaca acaaatatct ctttggtcca ataagataga aatttgccat
    22861 cctactacaa aagaaaaaat ggaatttaca tgtcatccac ctgacaaata tccatggaat
    22921 atattttaaa tctcaaatga ggtgattata tgagtaaaaa attttcaata ggagaaatgt
    22981 ctaaacttca taaaataagc atacagacac taaggtacta tgaccaaata gatttattta
    23041 aacctgttca tgttgaccaa gaaagcaatt acagatatta ctcaattgac caattttcac
    23101 aattagatat tataaaatat ttaaaatatt taggtatgcc tttaaaagaa attaagtcaa
    23161 agcttgatga aaataatgaa gaaattctaa aattactaac aaataaaatt aatatagtgg
    23221 atgaaaaaat taaagaatta gagcttataa agaaggtact aagcacaaag aaagaaacta
    23281 tggaaaaagg tatgaaagta gatgaagttg gtaaaatagt gagaaaacat attccacgaa
    23341 gaagtatctt atctatagag tatgaagaag gattaagtag tgaagaaact atggaacttt
    23401 ccagaagaca aatagcaaat atattagaag ataatatatc tgtgttctat ggtggagtaa
    23461 gtggacttgt atctatagat aaatttttaa aagaagataa agttatttat gacaatagtt
    23521 ttgttactgt tgaaagagac ttatttgata taaaatcaca aaaacatata acagaaattt
    23581 catctggtga ttttatttgt atgacatata aagggcctta caaagaaaat tataccgctt
    23641 ttaagaaact ggttgattat gttgaatata aaagaatacc tattgaaaat tatatatatg
    23701 aaatacctat aatagaccct ctgtctacaa acaacgaaga ggaacttcta acagaaattc
    23761 aaatacaagt taaaaagaga gaaattgact taagatgtct agtgtagtaa tctagtttac
    23821 ttacacatct taatatagtt gtctattatc aacatatgat ttttctagat agtgattcct
    23881 aacagtgaga ttttcaaaaa ataggaggaa atatggctaa tgctaattcc ctcctatttt
    23941 taattaagcc atatcatttt aattaagcca tgtcatcacg aagtgcatca ataatatttt
    24001 ctttttttat cttattaata gtatacaaca ttgtaataaa tactataaat aaaacactaa
    24061 atatactaat tccaatacta aaccatggaa tcacaaaatc aatatcatct gtaaccatta
    24121 ctttatgaat tagccatgaa gaaattactg ctataggaag tccaaagaac aatgctctca
    24181 tgccataaaa ggcacactca aaattcatca tcttttggaa atcatgctca gacattccta
    24241 cagagcgaag catggcaagt tctctacgac gtaacttaat attagttgaa attgtattaa
    24301 acacattggc aatcgaaatc agagaaatca taattatgaa agtataagca aaaacattgg
    24361 caataaaaat gtaattacgg ttctcatcca tcattccata catattgtac agattgtact
    24421 cattcgtaac tccctcactc tcaataattt gctccatttc tgcaactgac tgagtaggat
    24481 ttttcgattg aaagcttaaa ctttcaattc ctgtagaaac accaggaatc tcaaacttat
    24541 ctttggctga ataaggagcc aatactctga agcttgcttt cttcacatct gaacttctga
    24601 ttcctggagg agaatcaggt ggcactactt tgacaaatga aatattcaca ttttgtctct
    24661 caggtatctc caacttgcca ttagtttcag gagtgaggct aacattcata gaagaactgt
    24721 tgaaaatatc aataatatta ttatcctttg ttaccataga aacagcaatc agttttgcat
    24781 tttgtccagt atattcttct gctggtaatc caagactttt aataatattc agataagttc
    24841 tatcatcaag aaattgcata tctactggta attcaagcat ttcactcata gaatgtgaac
    24901 cctcatagtt caaataatcg tctgtaagct cacttacttt ggcaacacat gaataggtca
    24961 taagtttctg atacaaacct tcgtaaacac catttgcagt ttttagctta tcgtaaagct
    25021 tcaacatttc gttgtctttc ataccttgtg tagaaaaaat aatatcctgg tcagtaacct
    25081 ctgacctctg atttgacatc tgttttaggt ctgctactaa agcacttgta gatataaata
    25141 gaactatgct taaaacaagc gacaatacaa tacttcgata acgtttcttg tttcttttaa
    25201 agtttttcat agcaagaact ccctcaagac cataaactcg ctgtgttagt tttgatgtct
    25261 tcacagattt agattcaatt tttacctcat ttgtctgacg aatacactcc atcactggtg
    25321 tgttggcagc ctttcttgct ggtatgtaag cagaaattaa gattgtaacc atacttacca
    25381 ctgctgcaac gatgattgca gggatagaca atgttaatgt caacggaacg tttacataga
    25441 gaatatttgc aaagttttta gctatgacag aaatcacaag tccaatactg actataccga
    25501 ttgcaacacc aattggtata ccaatcgcac caatgcaaat tccttcaaat agcaccgaat
    25561 ttcgtaactg ttttgctgta gctccaacag atgaaagtat tccaaattga tgtgttcgct
    25621 cgttcaatga catgttaaat gaattatata ttagaaaaat tgagcctagc atgattatgg
    25681 caagcacaat gccaccaacc gagtacaaaa acatattgaa caaattgtta tcagaaacac
    25741 ctatgaagcg caacacattg tcattgaaaa tggaagcatg gtctttagtg ctacttatgt
    25801 aagagcgaat ctgatgtgga tttttcaatg tgacaaaaag gctaagactg tcagtcttga
    25861 cttgtttatc ttctttggtt atcaaggtat atcctggtgc agaagattct tcaaatacgg
    25921 gctttctaca gatacccaca actgtataag ttttcttatc ctgtggcaca aaaacttcac
    25981 tcccagcagt gtatgggtca ctctgaccaa gcttttcatt ttcactcatt cgtttcccta
    26041 catccaaaga aatagtatca cctactgcaa atttgacacc accatctgtc gcaactttac
    26101 ttgaaacaag aatctctcta ctattttcag gtaatcttcc agaaattaaa gttataggca
    26161 aagtatcaaa agcttctttg ctaaatccag caataaaaag atatggttta tctggatttc
    26221 tgttaccttc aagtgttgca tagccaatat tttcaaatgt tgcagtattt gcaacttcat
    26281 cattgactct ttgttcctgt acaaaagaag aatccacatc caaaaactgg acatgccaat
    26341 caccatattt tataatagct ccatttgtca tatagttcat caaagaaact ccaaaggtag
    26401 taactgctgt aatcatagca gctgacagaa caactcctat aattgttaca agagttcgag
    26461 tgcgattctt tttcaatcct tgcagagcaa ctttgttaaa aatgttcatt aacgattcac
    26521 cttcctctca tcacgcacta cttttccatc tgaaatacag ataattctat ctgcctgtaa
    26581 agcaatgttt tcatcatgtg tgacaataat gagagtttgg tgatatttac tatggctctc
    26641 tttcagtaac tggataatct cttgtccatt tttactgtct aagctacctg taggctcatc
    26701 agcaagcata actgctggtg cattcatcaa tgcacgacca atggcaacac gttgctgttg
    26761 gccaccagaa agttgatttg gtaaatgtgt ttttcgttct tttaaaccaa gcaattctaa
    26821 caagtcattt aaccgttctt gattgacctt acgcttatcc ataagtatag gcaatgtgat
    26881 attttctact acatttaatg tgggtatcag attgtgaaac tgataaatca atccaacttg
    26941 tcgcctacga aaaatagcaa gtttttcatt gctttggcca tatacatctt gcccctccag
    27001 atatatcttt ccacttgttg gtacgtctac tcctgcaatg gaatgtagta gtgtagattt
    27061 accagaacca gaagaaccaa tgattgcagt aaactctcct ttttcaatag taagtgagac
    27121 atggtcaagt gcagtaacct tattttcatt cttaccataa accttacata aatcttcaat
    27181 ctttaaaaac gccattatat aagtctcctt tcatatttta taaatatatc ataggacttt
    27241 caacttacat ttaagtgaca tttaaatgag agattcgtca ctttggaaaa cgaataacaa
    27301 atattgcacc accttgtggg tgattttgtg cagtaattgt tcctccttgt ctcataataa
    27361 tcgtcttaca aagtgccaat cctattccat aaccggctgt gcttgaattt ttcccacgat
    27421 aaaatctgtt aaatagacat ggtaaatctt cttttttaaa gcctgctccg ttgtcatgaa
    27481 tagtaagttg agtaaacaaa aagttgtcct cacaaatgat atcaattttt ccattatcac
    27541 ccacactctc catgcagttt ttaaatatat tttgaattgc ttctgaaagc caatttaaat
    27601 cacccaaaat ccttactcct tttggtatat caatatgtaa agtgatattg tgcagttcca
    27661 tgggaatcaa aagttgatga agtgcagaac taatcaaatt attcacatca atttgttctt
    27721 tttgaaagac tacaatacct gcatctaaac gtgataattt taaaagggaa gtaatcagcc
    27781 agtccattcg tataagtaat tcctctgttt ctcgtatcaa ctcttttctc tcatcttcat
    27841 cagaagtatt ctctaacagt gacaaaataa gagttactga tgtaagagga gtgcgaagct
    27901 ggtgggctat gtcagctagt gagtcagcaa ggtgttcttt ttcttttttc aatgcgtaat
    27961 tttgctctct aatacgtaac gtcattttag ttatttcact ttgcagaata gaaagttcac
    28021 cctcatctga ttcagcaata tacagatggt tagcattatg aagcacaaga ttaatttgtt
    28081 ctgaaatctg tgcaatactt ttgtatcgat ttttagtaaa cacaaaaaat gctattccaa
    28141 aggtagtggc agaaacaata gaaagtattc cagtcataat actgattgca aatcccaggg
    28201 ctacagtgag aatagtaatt aataagaata aaatagcaaa ttttcgaaac tctctatttc
    28261 gaaacataac ttccccccaa tctataccct gtcccacgaa cagtcaaaat tatttgtgga
    28321 cttgaagggt catcttctat cttctctcgc aagcgtttga tgtacacagt caatgtgttg
    28381 tcattaacaa actcacctgc cgcatcccac aattcatcaa gtaatcttac cctcgtaata
    28441 atacttttag gattgctaat aaacaccaat aacaaacggt attctaaagc tgaaagaaaa
    28501 acctcattgc cattcttttt cacaacaccg gttgccgtat ctacataaag cccacaaatt
    28561 tcaaaagttg acccaaaacg cccacttttt cgtagtgctg ttctaattcg cgcaatcagt
    28621 tcacgtggac gaaaaggttt agtaatataa tcatctgcac ccatattcag tccagtaaca
    28681 acacttgatt catcaccgga agctgtcaaa aagataacag gaatgtcttg cttttctttg
    28741 atttctgtgc aaatcgtaaa cccatttcca tcaggtaaag aaatatcaat aagtgccaaa
    28801 tcaaatttat tcttagcaat ctctacaagt gcttcacttc ttgtggatac atgggttact
    28861 ataaatcctt ctgaattaag caaaagaata agatttttgg caattgcttt atcatcctca
    28921 actaaaaata tccgtttcat ttactttcat cctcctttat tttattagtt ttatatattc
    28981 cagcagagca atagtacact actattttac ttatcattac acaattttca gtagtttctt
    29041 ttgttcttat ataatccatg cataatacct ccattgattt attatatttc ttataacata
    29101 aatacacaag ataagacaaa aaacataatg taagtgcaag ttttttaact cactaactta
    29161 taaacataca ctcctactaa cactactttc ttgatgacac atcttacaag cttatattca
    29221 tacatcaatg aaaaattaaa tttatccatt tattaaaata aaaggcaata gcttctatag
    29281 ctaatgccct ttatcttaaa atgatttaaa tactatacaa tttttataaa tggctataaa
    29341 taaactcgta tagaatataa aatttatcta cagtcttttg ttatacaaaa tttataacct
    29401 cttatagaac aaatttacca ctttgcaaac ttatcacttt tactcagcta tattataaac
    29461 ttttaattta tctttgttta aatgtttctt cttaacttct actaaagctt ttagcgtatc
    29521 caaagatgtc attatttcag tagaaaattc aatggctgtt cttctcatct taaatccatc
    29581 tcttgtagag tcatttcctt tagttggtgt atttattact atatcaactt gtttatttct
    29641 tatagcatct aatatgtttg gtctaggctc ttcaattcta tttactatat cagcctctat
    29701 tccattttct cttaaactct tcgctgttcc ttctgtagca acaaaagtat atcctaattc
    29761 tttcatatct tttgctatct ctataaactc atctttatca tgattattta cagtagcaag
    29821 tactactcct ctttcatcag acatatgtct acctgctgcc aagaaccctt tatataaggc
    29881 ttcttctaaa ttttctccta cacctagaac ttctcctgta gacttcattt caggtcctag
    29941 acttacttca actttagata atttagacat tgagaatact ggtactttta ctgatactaa
    30001 ttttggctct ttgtaaactc ctgtactata tcctaaatcc tttaatttag ctcctaacat
    30061 acacttagta gccaagtcaa ctattggtac tccacttact ttacttatat atggaactgt
    30121 tctactagct ctagggttaa cctcaattat atataattca ttttgaaatt ctataaattg
    30181 tatattaacc ataccaagca cattaaggtc taaagctatt tttttagtgt attctactat
    30241 tttttctttt atttcatcag atatattttg acttggatac attgttgtac tatctccaga
    30301 gtgaactcca gctctttcta aatgctccat tataccaggt atcaatatat cttctttatc
    30361 acatatagca tctacttcta tttctctacc agttagatat ttatctataa gtactggatt
    30421 tttatggtct ctatcaaatg catcatccaa atactgaatt agtttttctt cgttataagt
    30481 tatttccata ccttgaccac caagtacata agatggtcta actaatactg gataacctaa
    30541 ctccttagca atttcaacac cttcattagt tgtccataca ccttttcctt ttggtctatt
    30601 tatatctagt ctctctagta aatcatcaaa tttttctcta tcttctgctg catctatatc
    30661 tctaaagtct gttcccagta taggtatatt tttttcatgt aaaaacttag ccaattttat
    30721 agctgtttgt ccaccaaact gtagtattac accttctggt ttttcttttt ctattatatt
    30781 taaaacttct tcttctgtta atggttcaaa atacaactta tcagatgtat caaaatccgt
    30841 acttacagtt tcagggttat tgtttacaat tatagtttct attcccattt ttcttaaaga
    30901 ttttacacag tgaactgaac aatagtcaaa ttctattcct tgacctattc ttattggacc
    30961 agaacccaat actacaactt ttctcttatc actaactact acctcatcat attgttcata
    31021 agttgaatag taataaggtg atagagcttc aaattctcct ccacatgtat caaccatttt
    31081 ataaactggt tgtatattat ataaacttct caattcatat aatttttctg ggcttatctt
    31141 cattaagtca gatatacctt tatcagaaaa tccctttttc tttaattcat gtaaatattc
    31201 tttatctaag tcttctattt ttagaacttt taacttttct tcttgttcta ctatccattt
    31261 aaatttattt ataaaccact tatcaacacc agttatttct gatatcattt ctactttata
    31321 gcctcttctt atcatttcag ctaaatcaaa tagtctctca tcatcaggta ctactactct
    31381 agcctttaat tcttctaatg ttctaccttc tgatggagca tgaaccaatg aatattttcc
    31441 tgtttcaagt gatcttatac ctttaagaat agcagcctca aagttacttc ctatactcat
    31501 gatttcacca gttgccatca tcttagttcc taattttcta ttggcatgtc taaatttatc
    31561 aaatggccac tttggtattt ttactaccac ataatctaat gttggctcaa aacaagcata
    31621 agttttttta gttactgcat tttctatctc atccaaagta taacctaaag ctatcttagc
    31681 tgctactttt gctattgggt atcctgtagc ttttgatgct agggctgatg acctactaac
    31741 tcttggattt atttctatta tcgcatattc taaactatgt gggtttaaag ctatttgaac
    31801 gttacatcca ccttgaacct ctattgcatt tattatgtct attgatgcct ttctaagtag
    31861 ttgatactct tcatcactta gagtttgact tggagcaaca actatactgt ctcccgtatg
    31921 aactccaact gggtctacat tttccatatt acatacagtt atacagtttc catttccatc
    31981 cctcattact tcgtattcta tttctttcca accttttata ctcttttcaa gaagaacttg
    32041 tccaacagga cttagctgta gtccatgtga aagtatttct cttaattctt cctcagtatc
    32101 agctatacct ccaccagttc cacctagcgt atatgctggt cttacaacta ctggatatcc
    32161 tatcttattg gcaaactcaa gacctgcttc taaatttgta actatatcac taactattac
    32221 tggttgattt atttctttca ttacttctct aaataaatct ctatcttcgc ccttttttat
    32281 agattctata gatgttccta taacttttac attatattta tctaatatac cagcatcatg
    32341 taattctaca gctaagttta atcctgtctg accacccata cctgcaagta aactatctgg
    32401 tttttctttt tctattattt tttctataaa ttctattgtt aatggttcta tatatatttt
    32461 atctgcaatt tctttatcag tcattatagt agctgggtta ctatttacta atacaacttc
    32521 aattccctct tcttttaatg cttggcaggc ctgtgttcct gaatagtcaa actctgcagc
    32581 ttgacctatt attattggcc ctgaccctaa tactaaagtt ttctttatac tatctaattt
    32641 aggcataact ctttctcctt atctaaacaa ttttcacagt tcttatcttc atcatctata
    32701 caatcaactt taattacttt attttatctt aaattcaaat ttactcatca atacatctat
    32761 ttaatacatc tatgttaatt aaaccaaact ttttgattta agttattctt gttttttagt
    32821 tagctattct aatgttattt attttactaa ttctaaaaat ttatcaaaaa tatagtcatt
    32881 atcttttggt cctgggcaag cttcaggatg gtattgaaca ctgtatacat ctagtttctc
    32941 atgtctcatt ccttcaacag tattatcatt taaatttata tgtgtcactt tcatttgttc
    33001 aggaacttct gaaacataat aaccatgatt ttgagaagtt ataaatactt ttccttcttc
    33061 taaatcttta actggatgat ttccacctct atgcccaaat ttaagtttag cagtttttcc
    33121 tccaagagct aaagctaaaa gttgatgtcc taagcatatt ccaactatag gttttttacc
    33181 aataagagct tttatatttt ctataacatc ttctaggtct tctgggtctc caggtccatt
    33241 tgacaagaat attaagtcag ggtttatact caatacatct tctggagatg ttgtagctgg
    33301 aaatattgtt atatcacatc cacgagctat aaatgatctt aatatatttc tttttactcc
    33361 aaaatccata acagccactt ttggaccatt tccctttata tgctctactt cttttcttgt
    33421 aacagttcta actgcttctg tatttgaaaa cttatccaat ttatgtttaa catcttctaa
    33481 tttactgtct tctaatgtaa ttataccttt catggttcca ttatttctaa gtatcttagt
    33541 caaagctctt gtatctattc cttcaagtcc gataacttta ttttgcttta aatattggtc
    33601 tatatcaatt tcacatctaa aattacttgg agaatcactt ttctccctta ctatgaatcc
    33661 ctttacttga actttttcag actctgctga tgataaattt ataccataat ttcctacaag
    33721 aggataagtc atagtaacta tttgaccata gtaagaaggg tctgttaaaa cttcaccata
    33781 tcctatcatt gaagtattaa atactacttc tcctacactt tcttctaaat atccaaatgc
    33841 ctttcctata aaaactgttc catcttctaa tattaacctt gctttcatac tacagtacct
    33901 cctataattc tctttagaca aactaaagaa cattaactaa atataagctt ttaaatatct
    33961 atatctattt ttcactttta cattctagtt ttatacttat ttacatttat tttttatctt
    34021 tttctattat actacaaatt tcttaaaaaa aagaagttat ctcaaaagta aaaactccta
    34081 gaaaaaataa cctctttttt ttatttaata attaaattct taaactatac aatcaactta
    34141 atttctattc agctatattg taaactttta actcatcttt atttaaatgt ttcttcttaa
    34201 cttctactaa agcttttagt gtatccaaag atgtcattat ttcagtagaa aattcaatag
    34261 ctgttcttct tatcttaaat ccatctcttg tagagtcatt tcctttagtt ggtgtattta
    34321 ttactatatc aacttgttta tttcttatag catctaatat gtttggtcta gattcttcta
    34381 ctctatttac tatatcagcc tctattccat tttctcttaa gctattagct gttccttctg
    34441 tagcaacaaa agtatatcct aattctttca tatcttttgc tatctctata aactcatctt
    34501 tatcatgatt atttacagta gcaagtacta ctcctctttc atcagacata tgtctacctg
    34561 ctgccaagaa ccctttatat aaggcttctt ctaaattttc tcctacacct aggacttctc
    34621 ctgtagactt catttcaggt cctagactta cttcaacttt agataattta gacattgaga
    34681 atactggtac ttttactgat actaattttg gctctttgta aactcctgta ccatatccta
    34741 aatcctttaa tttagctcct aacatacact tagtagccaa gtcaactatt ggtactccac
    34801 ttactttact tatatacgga actgttctac tagctctagg gttaacctca attatatata
    34861 attcattttg aaattctata aactgtatat taaccatacc aagcacatta aggtctaaag
    34921 ctattttttt agtgtattct actatctttt cttttatttc atcagatata ttttgacttg
    34981 gatacattgt tgtactatct ccagagtgaa ctccagctct ttctaaatgt tccattatac
    35041 caggtatcaa tatatcttcc ttatcacata tagcatctac ttctatttct ctaccagtta
    35101 gatatttatc tataagtact ggatttttat ggtctctatc aaatgcatct tgtaggtatt
    35161 gagataattt ttcttcgtta taagttattt ccataccttg accaccaagt acataagatg
    35221 gtctaactaa tactggataa cctaactcat tagcaacttc aataccttcg ttaagtgacc
    35281 atacaccctt tccttttggt ctatttatat ctagtctctc tagtaaatca tcaaattttt
    35341 ctctatcttc tgctgcatct atatcatcaa aatcagttcc cagtataggt atattttttt
    35401 catgtaaaaa tttagccaat tttatagctg tttgtccacc aaactgtagt attacacctt
    35461 ctggtttttc tttttctatt atacttaaaa cttcttcttc tgttaatggt tcaaaataca
    35521 gcttatctga tgtatcaaag tctgtactta cagtttcagg gttattgttt atgattatag
    35581 tttctatgtc catttttctt aaagatttta cacagtgaac tgaacaatag tcaaattcta
    35641 taccttgacc tattcttatt ggaccagaac ctaatactat cacctttttc ttatcactaa
    35701 caactacctc atcatattgc tcataagttg aataatagta aggagatata gcatctatct
    35761 cacctgcaca agtatcaacc attttatatg ctggtttgat attgtataga cttctcaatt
    35821 catataattt ttctggactt atcttcatta ggtcagatat acctttatca gagaatccct
    35881 ttttctttaa ttcaagaaga tagtctttag ttaaatcttc aattttcata acttttaatt
    35941 tttcttcttg ctctactatc catttaaatt tatttataaa ccatttatct atacctgtaa
    36001 tttgttctat catttctact ttatagcctc ttcttatcat ttcagctaaa tcaaatagtc
    36061 tctcatcatc aggtactaca actcttttct ttaattcctc aatgcttctc tcctcagatg
    36121 gtgtgtgaac taatgaatat tttcctgttt caagtgatct tatcccttta agaatagcag
    36181 cctcaaaatt acttcctata ctcataattt caccagttgc catcatctta gttcctaatt
    36241 ttctatttgc ttttttaaat ttatcaaaag gccattttgg tatttttgcc accacataat
    36301 ctatagttgg ttcaaaacaa gcataagttt ttttagttac tgcattttct atctcatcta
    36361 aagtataacc taaagctatt tttgatgaaa ctttagctat tggatatcct gtagcttttg
    36421 atgctaaagc tgatgaccta ctaactcttg gatttatctc tataactgca tattcaaaac
    36481 tatgtggatt aagtgcaaat tgaacgttac aaccaccttg aacttctact gcatttaata
    36541 tatctaaaga tgctttttta agcattgcac attctttgtt acttaaagtt tgagttggag
    36601 caacaactat actgtctcct gtatgaactc cgactgggtc tatattttcc atgttacata
    36661 caactataca atttcccttt gaatctctta ttacctcata ttctatttct ttccaacctt
    36721 ttatactctt ttcaattaat acttgtccaa caggacttaa ttgtagacca tgtgaaagta
    36781 tttctcttaa ttcttcctca ttatcagcta tacccccacc agttccacct agtgtgtatg
    36841 ctggtcttac aactactgga taaccaattg taagtgcata atcaagacct gcttgtaaat
    36901 ctgtaactat atcactaact attacaggct gatttatttc tttcattact tctctaaata
    36961 tatctctatc ttcacctttt ttgatagatt ctactgaagt acctattatt tttactccat
    37021 atttatctag tatgcctttt tcataaagtt caactgctag gtttagccct gtttgtccac
    37081 ccatacctgc aagtaaactg tctggtcttt ctttttctat tattttttct ataaattcta
    37141 ttgttaatgg ttctatatat attttatctg ccacttcttt atcagtcatt atagtggctg
    37201 gattactatt tattaataca acttcaatcc cttcctcttt caaagactgg caagcttgtg
    37261 ttcctgaata gtcaaactct gcagcttgac ctattattat tggtcctgac cctaatacta
    37321 aagttttctt tatactatct aatttaggca taaatttttc tccttatcta aatggttttt
    37381 actatctgta aataatacta aatctatatt aatgctaaga atttattaaa tatacaatct
    37441 aaatcttctt ttgttgtgta ttctggaatg tactgaacac tatatattgg taattcctta
    37501 tgtctcattc cttctatagt attgtcattt aaatttatgt gagttacttc tgtattttct
    37561 ggcatttctg aaacattata tccatgattt tgagaagtta taaatacctt tccagtttct
    37621 aaacatttaa ctgggtgatt acctcctcta tgtccaaata caagctttga agtctttcct
    37681 cctaatgcca atgctaaaat ttgatgtcct aaacctattc ctgtcaatgg ctttttgcct
    37741 attaattttt ttatattttc aactatatca tttatatcct ctggatttcc tgggccatta
    37801 gataataaga ttaagtcagg atttatattt aaaatttctt ctactgtagt cattgctggt
    37861 actatagtta tatcacagtt acaagcctta agagactcta gtacactatt tttaacacca
    37921 aagtctataa ctacaacttt agctccagta ccttttatat tttcaatttc ttttcttgta
    37981 acatttttta ctgaatcttt gttacaatgc tcttctaatt tagactgaac atcttgtaat
    38041 gatacatttt ctaaagttat aattcctctc ttagttccat tgtttctaat aattttagtt
    38101 aatgctcttg tatctattcc ttcaagtcct attaccttat tttgctttag atatgtgtct
    38161 atatccattt cacatctaaa attatttggg ttatcacttt tttctcttac aataagtcct
    38221 tttacttgca ccttatttga ttcaacatct tctaaattta ttccatagtt tcctataagt
    38281 ggataagtca tagtaactat ttgtccataa taagttgcat ctgtcaatag ttctccatac
    38341 ccagccattg aagtattaaa tacaacttct ccaatagctt cctttagata tccaaatgct
    38401 tttccttcaa atactgttcc atcttctaaa attaatttcc ccttcatgta agctacctcc
    38461 caaatatttt atctaagcat tttctatttc tcttacaata ttaatagctg attctcttgg
    38521 attttctgct tttgttatag gtcttccaac aactaaataa tgagcacctt tttttattgc
    38581 atctgatgga gttacaactc ttttttggtc tccaacctct gctgatttag gtcttattcc
    38641 tgggcaaact gttataaaat cagttccaca tgcttctact attttatcta cttcttgcgc
    38701 tgaacaaaca accccatcta ttcctgattc ttttgccagt ttagctctct ttatagctaa
    38761 ctcttctgtt gtcatattac atcctatgtc ctgtaaatct tggttgctta atgaagttaa
    38821 aactgttact ccaactacta atggcttttc tatatttaat ctttctgctt cttccctagc
    38881 aattagtgca cattgtctca ttccttccat atctgataca tgtattgtca ttaaccaaac
    38941 attatctctc actgcagctt tcactgcact tttcatagta tttggtatat catggaattt
    39001 aaggtctaaa aatatttttt tacctttttc tttaagataa tctattgttt taccttttgt
    39061 ggcaacgtat tgctctagcc ctaccttaaa tatgtctaca ctatcttcta atctgtctat
    39121 tagttcttta gctttatcaa attcgtcagt atcaattgca actatcaatt tttctttacc
    39181 atttatcatt tttacatctc ctttttaata aagcctaaat aatcataaaa aaatcctcac
    39241 accaaaagag gcataaggat tttatctata cataactcta ttcattagct atgagatatc
    39301 tttcccagtc tctctggact agtttaaaag actttattat atttaaattt ttattttata
    39361 ctcatcaaaa acctctagaa gatttactag aggttatata ctaacattat ctataaccct
    39421 ttagtctctc tggactactt taaggaattt agctgtaata tttttcttta tttatataac
    39481 tttatgtatc tataggataa tttaaagttt tatctttttt ataattaaac cactaattgc
    39541 tttttatgtc aataagtctt tgaattattc ttcatcttta tcaaacaatt ttttctcaaa
    39601 atatgattct gcattatata ttgcagcaac tggattatgc ccagttactc tatctttgac
    39661 aactaaagtt gttataggag cttccatata tttcatggct aaagtatcgt gaccaacaca
    39721 taatccaaga attacattaa ggtcaacctt agcttcattt aaaaataaag cttgtcctat
    39781 tggattgcac attacctcat ctgaacactt tgatattgtt tgagatgctt ctagtccaat
    39841 tgtactctta ggcattgccc catttttaca tataacagaa aatacttcaa acccatgatg
    39901 ttcaaatatt ttttgcactg tttgcatttc tctttttagt ccaatacaaa atgctatgcc
    39961 aatcttttta taaccacact ttttagcaaa ctctataatc tcttttagtc tagtatattg
    40021 acaatacccc tctgcttcaa ctaatccagc attataagct atttttctat tctcttcttc
    40081 ttcataaagt tgctttgctt tttgttgtag ttcaatttct ctagttggac atatcttcat
    40141 taatttatcc atatttccat tagaacagcc tctaagatta cattttccac acttatacat
    40201 atccaatatc cccttccaaa attttattca tcttgcgatt cttctttttc tagagttaca
    40261 ttttcttctt gggtttcttc ttctggtttc acatgctcga ctacagttat aaaatatgat
    40321 tttcctattt tattccttat aggaagtttt tcatatgaaa catctatttt attttttata
    40381 caagctatat caaaatgtct taaggctaca cctaagctaa ccttattcat atcttcacag
    40441 tgtttcttag gtctcttttc aaataaatga agacctcgtt catccttatg gaaataccac
    40501 ggttgagaat tttttacaga tggagctctt cttgtcaatt ctaagatatc tttccacttt
    40561 ctattcctat ttatcttatt tatcatatct tttactggct ttctatcagg tctagcatat
    40621 ctcgtcctaa acaactcttc attttttgct ggatatccaa atgctattat tatataagga
    40681 ttttctattt tgttttcatc atcttcttct gtttttattc cagctagata atctaaatca
    40741 tcttcctcat cttcttcaaa attcttattc ccaatatcta caaattccaa gatatcttct
    40801 ctctttaagt ttccttccaa ccagcaagta gctattccca atgttgtaag gcgcaaaact
    40861 acaccttctg tagcaaatcc tatattctgt aaatagtcct cacctttgtc tgatgtaact
    40921 actatataat gaggtgcctt tactttacat tccttaccca tcaaaaaatg tattatatga
    40981 cctctatcaa caacatgtgc tttaatattt aaatcttcat ttaagtattc taaattttta
    41041 caacttcttt ttatttcttc catcaatggc ttttttatct tcttactgga aaaatttctt
    41101 acagattttc tataaaaaat ggcttcataa agctccattt atgccctcct aactgaaatc
    41161 ttctgacgta taaactctat aaatttatat aaatacttta tattttttat agttttattt
    41221 cctttttcct cctgccctat tttgaaattc ataagaatat ctactctagt ttaatatagc
    41281 attcaaaagg cttaaattct accttatcaa tgtctataca ttagcataat ttttttattt
    41341 gattttctat gctaatttaa ataacaacac tagctattac ctttttaatt aagctttgcc
    41401 agaaaaattt tttttcattt ataaatatct atctcattat tatattataa gtttttttcg
    41461 gtattcttgt atagcttttc actaaaaaag tatttttatt tctactttaa tattattatc
    41521 aacaaaaaac atactgctta aataagtttg ttatcctcat aatttgtttt aattaaaata
    41581 ttttttttat ttctcaatct ataaagtata aatattttga atatgaccca aaatctaagt
    41641 aaagttataa tattttataa gttgttcaat attaaaaaca taagtaatgt tataatactt
    41701 tataagttat tcaatattaa aaatatagga ggataaatat gaatttaaat agaaatttag
    41761 aaaaatacgc tgaattagca ataaaagttg gagttaacat acagccaggt caaattctac
    41821 ttataaagtc accaatagaa tgtgctgatt ttgcaagaaa tgcacttaaa gaagcatata
    41881 aatgtggtgc taaaaatgtt tatatagagt ggtctgatga agaaagtact cttattaaat
    41941 acttatatgc tccagatgaa gcatttcatg agttccccaa atggacagca gaacaatatg
    42001 tagatatagc aaaagaaggt ggggcatttt tatcagtcta tgcccaaaat ccagatttat
    42061 taaaagatgt tgaccctgaa agagttgcaa attttcagaa ggcatctgga aaagctttaa
    42121 aagaatggcg ttcttacact ctaactgata aatgtaaatg gaatatagtc tctgtgccaa
    42181 caaaagattg ggcaaaaaaa gtgtttccaa atttatcaga agataaagca atagaaaaac
    42241 tgtgggatgc tatatttaaa tgttcaagag tagatggtca ggaccctata aaagcatggg
    42301 aagaacataa tgaaaatctt aaatctaaaa tggactttct aaataaaaat aactttaaaa
    42361 ctttaaaata taaatcctca aaaactgatt tgactctaga cttgccaaag ggtcatgttt
    42421 ggctaagtgg agcatccaaa gaccctaatg gaataagttt taatccgaat attccaactg
    42481 aagagatttt tggaatgcca cataagttta aagtaaatgg aactgtttat agtacaaaac
    42541 ctcttgttta tggtggaaac ataattgaca atttcttctt aacatttaaa gatggtaaaa
    42601 taatagattt ctctgctgaa aagggactag atacactaga aaaacttatt gaaacggatg
    42661 aaggctctca ttatttagga gaagtcgctc ttgttccata caactcacca atatccaata
    42721 cggatataat tttttataat actctatatg atgaaaatgc ttcttgccac tttgcaattg
    42781 gttctgctta taagacttgt ctggaaggtg gaaataattt aaaagatgaa gaattggatg
    42841 aacatggtgt aaatgacagt ttaacacatg tagattttat gattggttct gctgatatgg
    42901 atattattgg tgagacttat gatggaaagc aaatacagat ttttaaaaat ggtaattggg
    42961 ctttttaaca tatgatttta actaaaaagg gctgatggaa gttataccag acttcccagc
    43021 cctttttaat gttgatgata aatatttttt atattaatta atctcattat tatagattaa
    43081 ttcctattgt tttaaacata agtatctaaa acttatattt atattatata taaaatatcc
    43141 tatttagtaa aacctttatt ttaatttata tgctaaaaat ttaactttgc tatttcaaca
    43201 gcagactttg catcctttga ataataatca gcacccattt cttcagcgta ttcttctgtc
    43261 aaaactgcac ctcctacaaa tacctttgca tttatctcat tatctctcag tgcctgaatt
    43321 gtatctttca tactttgaac agtcgtagtc ataagtgcac ttaatcctac taattttata
    43381 tttctctttt tagcaacttc cactacttct tcaataggga catctttacc taaatctata
    43441 acttcatatc cataattttc tagcattatt tttactatat ttttcccaat atcatggata
    43501 tcacctttta ctgtagcaac tattattttt cctttggata cattattgct acttttactt
    43561 aataaagttt cctttattgt atttaatgaa acttttaccg tttctgctga ttgaatcaat
    43621 tgtggtaaaa atatatctcc actatcatat ctttttccta ctttatcaag agcaggaatt
    43681 aaaatctcat ctaatatata attttcatcc ttctctttta aaagattcag tgttacattt
    43741 tttgcttcat cttttaatcc tctttcaact aaaatatcca agggcaaatc tttatcaatc
    43801 tttgtagatg aatccgattt ttttgaatta tttatattgc tatattgatt tatatagttt
    43861 atacatcctt tatctgtgtt atttaatatt ttaaaagaat tgatagcttc catcatacta
    43921 tattcatttg gatttataat tgctaaatca agccctgccc ctaaagctaa tgttagaaaa
    43981 gaagcattaa gtgctttcct atttggaaga ccaaatgaaa tatttgaaac acctaatata
    44041 gtttttactc ctagtgtctt taccattgtt atagctttta tagtttctat tgcttcttct
    44101 tgttgtgctg aaacagttaa tgataaacag tctataaata tatcttttgg ttttatccca
    44161 taagaaactg ctctattaac tatccttttt gctatttcaa atctttcttc agccttttta
    44221 ggtatcccct tttcatcaag tgtaagtcca accacacatg aaccatactt tttaactatt
    44281 ggtaatatac tttctaaaga ctcctctttg ccatttacag agtttactat tgttctacca
    44341 ttataatatc taagtccttg ctctaaggcc tctacattgg aagaatcaac ttgtaaaggt
    44401 gtatctacta ctgcttgaat ctctcttatt aattttggca ttagtttttt ttcatcaatc
    44461 tctggaagac caacatttac tccaagtatt tgtgctccag catttacttg ctctaaacct
    44521 aaatttatag cataatcaac attttcattt tttaatgctt cttgtagaga ttttctacct
    44581 gtgggattca atctttctcc aacaactgta ggcgattgta cttccacaaa ttttgatggt
    44641 gaacagacta cacatttatc aattttctcg atacatccct ttgtaacaga atttatattc
    44701 tcagatattt cttttataaa acttggattc gtaccacaac atccacctat tatagtagca
    44761 ccaacttcaa cgaacttttt aaccccttca aaaaattctt ttgcatcgac atcatatata
    44821 gcctctccat caactatatt tggaagacct gcatttgctt gaaccataac agggactgta
    44881 gctcttgatg ctattttttc aactattggt aaaagttgtt taggtcctaa tgaacaatta
    44941 actcctattg catctacacc tagaccttct atagtagcca ccatacattc tggcatacat
    45001 ccagtaaaac ttcttccacc ttcatcaaat gtcatagtac agaaaatagg taaatcagag
    45061 ttttcttttg ctgctaatac agctacttta gcttcatata aatccatcat agtctctatt
    45121 actattacat caactccaga tttaacgcct tgtagtactt gtcttttaaa tatttcatat
    45181 gctttatcaa aacttaaagt acccataggc tctagcattt ctcctatagg accaatatct
    45241 aaagctacat atcttggttt agtattatct acattttcta tagcttttct agccaccaaa
    45301 acagcattgt caatcacttc ttctacagta tacttactat ctaattttag ttcattacat
    45361 ccaaaagtat ttgtaagaat tacattcgaa cctgcctcta agtatgctgt atgtatttct
    45421 ataagtttat ctggattttg aagaccaaat acttctggat tttcacctaa ctttaaccct
    45481 ttttgttgaa gcattgttcc cattgcacca tcaaatatta atatattatt tttcagatat
    45541 tgtcttattt ccacaagagt tcgctccctt tctatatgta cagtttctat agtttttaca
    45601 attttcacac gacttttctt tcttagattt atttattctt gtattagaaa tacctataat
    45661 agccactact gactttctcg gtatcataat tccattagaa gtaattgtta gacctatttg
    45721 tttttgagca ttcaaaacgt ttaatatatc tatatttttt tctattggta aatctccata
    45781 tcctgggcta tatcgcatag ttataaattt atcttgcttt aaaaaaacct tttctaaatt
    45841 attttgaaca atgtcacaca cttcttctat tgctgttgtt ccacatgaat caattattag
    45901 tccttttgtt aaatttgaat aagagtgtcg tcttatttct ttttctatat ctatccctat
    45961 agtagctgcc ataattacac actcattaca ttcttttaaa agttcatata catatttact
    46021 ctctaatgtt aaatttgttc cctctaattc tacaacatta tctttattta ttctaatagg
    46081 ataaatccta gataaatatc taggatttat tttattttta gttatagcta cacattcatc
    46141 aatagtatat ttaaggtctt tatctatctc ttgacctctg tattgtaaat atcttaaaac
    46201 ttcactttga tttatgctta tatcttctct tccaattaaa ttattctcca tatatattta
    46261 aagaaaatcc tttatctaaa acatcttttg catattcctt tgccccaaat aaagataagt
    46321 ctctgtagtt tccacattct atagctgtag ctgctggcat tttatctgat tcaagaactt
    46381 tttttaatgc ttcttctaaa ccaatcttaa ctgttttggc atctacatct ccccatagta
    46441 ttaaataaaa tcctgttcta catcccattg gagataagtc tattacacca tctaatgtat
    46501 ctcttaaata agtagctaaa agatgctcta aaccgtgcat agcagcagtt ccaaaacttt
    46561 ctacatttgg ctgtaaaaat ctcaagtcaa atttagttac tttatctccc tttactccat
    46621 caagtacact acactttctt acaaaaggag cttttacttt tgtatggtct agcttaaaac
    46681 tttctacttt ttccattatt taatcctcca ttatacttta aaatttgtta cattaattct
    46741 aaaaaaaact cttcctatct attattgata agaagagtat attatttcaa gtcatctcat
    46801 cgttgttagc aggaccacct gataaaaggt tggtatagga tcactgagct aactctctcc
    46861 cctaactctt gataagtatc ttattaaact gacttaaatt atatattact tttttatttt
    46921 tgtaaacact ttattgttat aatatttata tcacataatc gtacttttat tttataacta
    46981 agctgattgt attattggca gttgtacttc aaacacagtt tgatatttat cgcttgaaac
    47041 agtaatttct ccatcatgta aatttacaat attttttgta atcgcaagac ccaatccaga
    47101 acctccaaca tttctatttc tcgatttttc aactctataa aatctatcaa atatatttgg
    47161 taaatctaac actggaattg gttccccata atttatcact tttacaaccg ccatatcttc
    47221 ctttttctct gtgactatat ctacataatg cccatctttt ccatatttca cagcattagt
    47281 tataagattt tcaaatgctc ttaccaattt atttggatca gcttttacaa ttaatttttc
    47341 ttcattaaaa ttaattctac taaccataaa ttcttgttta aaataaattt caaattgaga
    47401 tactacttga cttaataact ctactaaatt tatttctatt ttgtttaatt ttattgtatt
    47461 attttgcatt ttagtcaatt caaacaaatc atttataaga acatttaaat ttttcgattt
    47521 ttcatatgca atatcaacat agtacatcat ctgaacttca tctttatact taccttcttc
    47581 tatcactcct aaataaccca ttatggatgt caaaggtgtt cttaagtcat gtgacacatt
    47641 tgttattaaa tctgtttttg tctgctgagc ttgtctctct tcaatagtta tatttctaag
    47701 ttgatggact atattattga ttttatgtgc tagttcatca atatcatcct tttctttatc
    47761 ctttaatata tctaaatcat actcaatatc tatacttttt tctaagttac cttcatccat
    47821 taaatttaca gcacttttaa tgatttctaa tttttgattt tttctattag tctttcttat
    47881 aaaatctaca atttgcttat ttctcttgta cgatataatg gaataaattg ctaatgatgt
    47941 gattgtaaat aaaaatggat aaaataaatc ataccctata agacttctga aatctcttgc
    48001 ttgatataca agttgattaa ctacagacat atcactattg acatataatg attttattat
    48061 atacatactt gttaaaataa gcattgaagt taaagcacag cttattataa gatctaatat
    48121 gggtcgaaac atatgattta ataaactttt atttttcaat tttatatcct actccccata
    48181 cagtatgaat tattttttct ccatctgtat atttttcaat tttatccctt attctgctca
    48241 tatgaaccat tacagtatta ttagattggt aatatttctc tttccatacc ttttcaaata
    48301 tttcttctgt actatatact ctgcctttat tatttgctag taaatataat atatcaaatt
    48361 ctcttgatgt caaaattatt tcatttccag ccactgttac actatgtttt tccttatcta
    48421 taattactac tccaaccttt atgacatctt tatcttcatt attagcacca ctaaaataat
    48481 atgctcttct aatcagcgac ttcactctca cagtaagttc aagtgggtta aatggttttg
    48541 tcaaatagtc atctgcacca gtcattatcc cttgtatttt atccatgtct tctgattttg
    48601 cacttaacat aagtattggt atattcaaac tttctctaac ttttctacaa acttctatcc
    48661 catccatttt aggcatcatt atatccaata tcattaaatg aatattttcc ttttttaaaa
    48721 tatctagagc ctcttttcca tcttcagctt taattacatt atatccacta tttaaaagat
    48781 atatctctat aaggtctctt atttcctttt catcatccac aacaagaact gtattattca
    48841 tttttacaca tcctaactta atctttgtat atagctttat tcatataaaa ttatactata
    48901 ttttagttag tgtaatctat aaatttatta atttctaaat ttccaatata atacaaatta
    48961 tttacacatt tatattattt attttgtaaa tattcttcta aagttaatcc ttttgaataa
    49021 atttcctcag cagcatcctt accaacatat ctaatatgcc atggttcata tgctgtacct
    49081 gttatatctt ctttaccttc aggatatctt attataaatc caaacttgtg agcattttct
    49141 gccaaccatt tagcttcttt tgtacttcca acaaaccatc tttctggatt tgttaaatca
    49201 atacaaagtc cagtttggtg ctcactgtaa ccaggctttg ctacatatgc atctgccttt
    49261 tctcgcccct gagattttac ccttcttgta taagtatcca gttgagtatc ataagatctg
    49321 tatgctgaag accctaaaaa ctgtattcct tctgaattag cttgcttaac taaatcttca
    49381 actgcctttg caggttctcc tgccatttgt ttttcttcct ctgttgcttc ttcaacaaaa
    49441 ggtatgttta ccttagtaat attttcagga gtatagtttt tgctaattcc atttgtcttg
    49501 tttaccaata atatatttga tatactattt gagtgatttt tattttgaga atttgcctca
    49561 acatcttgtt ctgaatcatg ttgagcagct actattggat ctttatcttt cttaagtaat
    49621 ctatctttag atataaatac acttataaat actactaaaa taatggctcc tattaattta
    49681 aattttttca taattaaatc ttctcctttt tgttatgttt gctataatgt taattttaga
    49741 gtataagact taagtatttt ataagtaaat cttaaataaa tcttaaattt aatattttta
    49801 aaggatggtg ataaaatgtc tatacaaaaa cttctaagta aagctcgtaa ggctatacaa
    49861 gattttgata tgatacaaga aaatgataaa attgcggttg gactttctgg tggaaaagat
    49921 agtttaactc tacttcatat acttaaaagt tatcaaagat tttctccaca aaactttgaa
    49981 ttgatagcca taacattaaa tactggaggt gttgataatt ctccactaga taaattgtgt
    50041 aaagaaataa atgttccttt ttatgaattt caaactgaca taaaagaaat tgtatttgat
    50101 ataagacaag aaaagaatcc ttgtgcattg tgtgctaatt taagaagagg tgctttgaac
    50161 gataatgcta aaaaacttgg atgtaataaa gtagctcttg gtcatcataa agatgatgca
    50221 attgagacat ttttaatgtc tatgttttat gagggtagag taaattgttt ttccccaaag
    50281 acatacctag acagacagga tttaacagtt ataagaccta tggtttacat agaagagtat
    50341 atgactaaga aaatttcaaa agattctaac tatcctatta tcacaaatcc ctgccctgca
    50401 aatggtcata ctaggagaga gtacataaaa aatcttattg ctaatttaaa taaagagatg
    50461 cctgatttta aaagaaatgt atttggtgct ttaaataatt ctgagaaatt gtttatatgg
    50521 gataaagaga agataaaaaa ttttaaataa taattaaaat ttttaaataa aaaagcaaga
    50581 ttgtattaat tgtaaataat ctttatataa tctacacaat cttacttttt
//
LOCUS       DAMJVI010000016        49368 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.016, whole genome shotgun sequence.
ACCESSION   DAMJVI010000016 DAMJVI010000000
VERSION     DAMJVI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.016"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(312..725)
                     /locus_tag="PC545_001026"
     CDS             complement(312..725)
                     /locus_tag="PC545_001026"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDF2455815.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRI"
     gene            complement(766..1896)
                     /gene="rodA"
                     /locus_tag="PC545_001027"
     CDS             complement(766..1896)
                     /gene="rodA"
                     /locus_tag="PC545_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDF2455816.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNLLGRYYKALYIISLILLAIVLLPGIGT
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVVVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQEDFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            complement(1981..2265)
                     /gene="minE"
                     /locus_tag="PC545_001028"
     CDS             complement(1981..2265)
                     /gene="minE"
                     /locus_tag="PC545_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDF2455817.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            complement(2283..3080)
                     /gene="minD"
                     /locus_tag="PC545_001029"
     CDS             complement(2283..3080)
                     /gene="minD"
                     /locus_tag="PC545_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDF2455818.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            complement(3104..3787)
                     /locus_tag="PC545_001030"
     CDS             complement(3104..3787)
                     /locus_tag="PC545_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDF2455819.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKIEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            complement(3885..6863)
                     /locus_tag="PC545_001031"
     CDS             complement(3885..6863)
                     /locus_tag="PC545_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDF2455820.1"
                     /translation="MEENKQVDRLKIIKIVMGVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKSLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMLTSMAALENGLD
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKLDVKEDRIEEV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            complement(6875..7363)
                     /gene="mreD"
                     /locus_tag="PC545_001032"
     CDS             complement(6875..7363)
                     /gene="mreD"
                     /locus_tag="PC545_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDF2455821.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGVNALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFVKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            complement(7378..8271)
                     /gene="mreC"
                     /locus_tag="PC545_001033"
     CDS             complement(7378..8271)
                     /gene="mreC"
                     /locus_tag="PC545_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDF2455822.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDVANFKDNSKKVKKLEEENEKLKKNMIA
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISASVVGKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            complement(8281..9291)
                     /locus_tag="PC545_001034"
     CDS             complement(8281..9291)
                     /locus_tag="PC545_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2455823.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     INMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            complement(9412..10071)
                     /gene="radC"
                     /locus_tag="PC545_001035"
     CDS             complement(9412..10071)
                     /gene="radC"
                     /locus_tag="PC545_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDF2455824.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGNKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKSTQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSIGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            complement(10116..10700)
                     /gene="maf"
                     /locus_tag="PC545_001036"
     CDS             complement(10116..10700)
                     /gene="maf"
                     /locus_tag="PC545_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDF2455825.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLGDLLKKYFSINLFYGV"
     gene            complement(10920..11891)
                     /locus_tag="PC545_001037"
     CDS             complement(10920..11891)
                     /locus_tag="PC545_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HDF2455826.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     IAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            complement(11907..12482)
                     /gene="rsxA"
                     /locus_tag="PC545_001038"
     CDS             complement(11907..12482)
                     /gene="rsxA"
                     /locus_tag="PC545_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDF2455827.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            complement(12497..13090)
                     /locus_tag="PC545_001039"
     CDS             complement(12497..13090)
                     /locus_tag="PC545_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDF2455828.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            complement(13103..13672)
                     /locus_tag="PC545_001040"
     CDS             complement(13103..13672)
                     /locus_tag="PC545_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HDF2455829.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMKEDTYKGLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            complement(13672..14649)
                     /locus_tag="PC545_001041"
     CDS             complement(13672..14649)
                     /locus_tag="PC545_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HDF2455830.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFTKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADISSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            complement(14672..16009)
                     /gene="rsxC"
                     /locus_tag="PC545_001042"
     CDS             complement(14672..16009)
                     /gene="rsxC"
                     /locus_tag="PC545_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDF2455831.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKPIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTITGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDIQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            complement(16225..17169)
                     /locus_tag="PC545_001043"
     CDS             complement(16225..17169)
                     /locus_tag="PC545_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455832.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKKGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIESISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            complement(17280..18554)
                     /locus_tag="PC545_001044"
     CDS             complement(17280..18554)
                     /locus_tag="PC545_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2455833.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSSQNNPTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGDVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGTNNSSNSNSTNNSDKKPSSEESIEGKNGKVTSAVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKSNGWYKVKLSSGTIGWVSASYISETNEDTKEKPNSSSN
                     QNSQSNSNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            complement(18686..19057)
                     /locus_tag="PC545_001045"
     CDS             complement(18686..19057)
                     /locus_tag="PC545_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2455834.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            complement(19285..19884)
                     /locus_tag="PC545_001046"
     CDS             complement(19285..19884)
                     /locus_tag="PC545_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455835.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKLKS"
     gene            complement(20085..20297)
                     /locus_tag="PC545_001047"
     CDS             complement(20085..20297)
                     /locus_tag="PC545_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDF2455836.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDDVSIEKLKEVIAELGFELKGIE"
     gene            complement(20370..22085)
                     /locus_tag="PC545_001048"
     CDS             complement(20370..22085)
                     /locus_tag="PC545_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2455837.1"
                     /translation="MKRIKVLTVVLAITLMVAGCSNTKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKNITSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQNHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKAIECLAKVEYANVNSVNQNDNKVKNQKKSAKKNSKKLTPEEEAKAKKAEE
                     DRIKKEAEEKKNREKEEVKKSLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKNYDLALVGWKLSIIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSNSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(22085..22639)
                     /locus_tag="PC545_001049"
     CDS             complement(22085..22639)
                     /locus_tag="PC545_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HDF2455838.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEHYNKIYGKKGKN"
     gene            complement(22632..23234)
                     /locus_tag="PC545_001050"
     CDS             complement(22632..23234)
                     /locus_tag="PC545_001050"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDF2455839.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNIPIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKELNNLTHPRIREKIISEIEKLR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALSRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(23243..25891)
                     /gene="polA"
                     /locus_tag="PC545_001051"
     CDS             complement(23243..25891)
                     /gene="polA"
                     /locus_tag="PC545_001051"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDF2455840.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKDYKAGRKGMPDELAEQLQPLKDLLDKFRINR
                     LEIDGYEADDIIGTVSKKAEENGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLIENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLDSMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSDDTEHVEEKIEILKLKDIEKFIDEVNKKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITIAEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNMDVHLDVDLNVGSSWYETK"
     gene            26043..26130
                     /locus_tag="PC545_001052"
     tRNA            26043..26130
                     /locus_tag="PC545_001052"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26077..26079,aa:Leu,seq:caa)
     gene            complement(26317..26760)
                     /locus_tag="PC545_001053"
     CDS             complement(26317..26760)
                     /locus_tag="PC545_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455841.1"
                     /translation="MKKNMLYVGIIYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERAVDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMCYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKRL"
     gene            complement(26763..26960)
                     /locus_tag="PC545_001054"
     CDS             complement(26763..26960)
                     /locus_tag="PC545_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455842.1"
                     /translation="MKNRLEEIRKERGIKQEELASILQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDMSIEEIFIYEEE"
     gene            complement(27152..28018)
                     /locus_tag="PC545_001055"
     CDS             complement(27152..28018)
                     /locus_tag="PC545_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2455843.1"
                     /translation="MEWINKLNQAITYIEENLESGVDYAEVAKIACCSTFHFQRMFSY
                     IAEVPLSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNIEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPVLEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYANLPDIELYPNGDINAPDY
                     TTEVWIPIQKPS"
     gene            complement(28244..29113)
                     /locus_tag="PC545_001056"
     CDS             complement(28244..29113)
                     /locus_tag="PC545_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2455844.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDVRIMNRSEVFNNLDKVAGYKGNMIDSPSYIIILSDIKDNYIENSGYIGESIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGINSDKEVTGIIAIGYGENVSNAKVLNATKTGQNYSK
                     SDMQVVTDKGSTRLGVEDVVYMNEWGNVASIEILEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVVLAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFDLKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(29619..29840)
                     /locus_tag="PC545_001057"
     CDS             complement(29619..29840)
                     /locus_tag="PC545_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455845.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEENEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(30342..31322)
                     /locus_tag="PC545_001058"
     CDS             complement(30342..31322)
                     /locus_tag="PC545_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455846.1"
                     /translation="MGDTSRFYESVKNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDSLSWKQIVILAECDGKNTTDDIINILSNRYRNIHNMKEKVMEFFMFYENV
                     YLTFEDDIQTLSSVFEITGNKSYITPLYFTIEIDGDNNTNDYFSEITSLLNCMYEKGC
                     RFIEVIGEDILKNKSMREIFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSKNDDTRIKLKEDIKITSLIKRGHTVVRGDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSDGSVKSYSFQKDKRIYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(31486..32358)
                     /locus_tag="PC545_001059"
     CDS             complement(31486..32358)
                     /locus_tag="PC545_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2455847.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPKEFDN
                     FKIVQVSDLHNKVFDKNNKVLIDKIESLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINMENESVRIYRGNSHLNLYG
                     LIIPLECYPYLFANNKKLELSDNFVEDNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRIPFVGGVLSPNREFFPKYDWGKYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITFKYEK"
     gene            complement(32522..33073)
                     /locus_tag="PC545_001060"
     CDS             complement(32522..33073)
                     /locus_tag="PC545_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2455848.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQKGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(33093..33995)
                     /locus_tag="PC545_001061"
     CDS             complement(33093..33995)
                     /locus_tag="PC545_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2455849.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCVSCNRCIDVCPQNISSLFDRAQINNKCDLCGECIKVCLKDAICIMT
                     EEMSVEEIVEEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYDKGINIAIET
                     CGYFDWNKVNDVFEKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPGGKMELLPYHKFGIDKYKALGLEDYIYEFDD
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(34010..36382)
                     /locus_tag="PC545_001062"
     CDS             complement(34010..36382)
                     /locus_tag="PC545_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HDF2455850.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDKRTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKILVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISVSENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSKQETDEKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDSAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLKQLAYLVKSSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMIKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(36603..37292)
                     /locus_tag="PC545_001063"
     CDS             complement(36603..37292)
                     /locus_tag="PC545_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455851.1"
                     /translation="MKVKRNINRVAIIVALNLSLALMVGCMSPVYDKNLSSSEQISKD
                     SLKEQVEDKKDESIELFNNYSQKINDAKGREEVKKDLSQRFSKAIIDSGYSITDSKSD
                     GSFVVSINGAEKPPEKNIRSLFYSMSEDDKEGKVTIDILCAKEYPEDDKLSESDKYIK
                     FIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKVKQSTKVLKL
                     SLVYKFDISNH"
     gene            complement(37533..38912)
                     /locus_tag="PC545_001064"
     CDS             complement(37533..38912)
                     /locus_tag="PC545_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2455852.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIIQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKMGYIKYSIFFYLFSVY
                     YKCLDAVIYRILFFAITGNVISSKESLLVPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLVNTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDNLRNNSKEINEYVNNLKDEIKTFKYIYNTGNMILDIIINEKSEICLKKGIKFTCS
                     INFSKVNFVKPIDVSSIFSNILDNAIEACDKIVDENVNKYIRIKGTMTRSFFVLKCEN
                     SKLNQLTFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELLFENSIDKFILNIYIPL
                     DQDTDSWGD"
     gene            complement(38906..39619)
                     /gene="rgbR"
                     /locus_tag="PC545_001065"
     CDS             complement(38906..39619)
                     /gene="rgbR"
                     /locus_tag="PC545_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HDF2455853.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILKDISIEYEIQKYISGEELL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFVTSLIDYVQEGYEVRAYRY
                     LLKPIELEELKKHVLTCIKDIEINKESHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLGKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTAILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(39869..40429)
                     /locus_tag="PC545_001066"
     CDS             complement(39869..40429)
                     /locus_tag="PC545_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HDF2455854.1"
                     /translation="MSAMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYKINKFGGI
                     FYFHKGSPRKIKYTVDYYYDVENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGYGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(40525..41328)
                     /gene="zupT"
                     /locus_tag="PC545_001067"
     CDS             complement(40525..41328)
                     /gene="zupT"
                     /locus_tag="PC545_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDF2455855.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGSWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGIFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(41479..42642)
                     /locus_tag="PC545_001068"
     CDS             complement(41479..42642)
                     /locus_tag="PC545_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2455856.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     IGIPYVSAGGTGVIATIKGANPGKTVALRGDMDALQVVECTDVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKQIPGILERIAKS
                     TAEAYRADAELEYGYLTPAVINDKECSKIATDAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(42702..43889)
                     /locus_tag="PC545_001069"
     CDS             complement(42702..43889)
                     /locus_tag="PC545_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455857.1"
                     /translation="MEAITAFCLVLVSLTIGDIVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTDEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVICLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIVGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(44252..45952)
                     /locus_tag="PC545_001070"
     CDS             complement(44252..45952)
                     /locus_tag="PC545_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455858.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIKDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPDGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(46207..46482)
                     /locus_tag="PC545_001071"
     CDS             complement(46207..46482)
                     /locus_tag="PC545_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455859.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEVEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(46601..47173)
                     /gene="mobB"
                     /locus_tag="PC545_001072"
     CDS             complement(46601..47173)
                     /gene="mobB"
                     /locus_tag="PC545_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDF2455860.1"
                     /translation="MLLLDSFMKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGY
                     RVGVIKHTCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKEKNPLYKMIQL
                     YEDMDLIIIEGYKNYRFKRLEVTRKGKYEDILSNKSDLIGIVSDIEYKLNIEQFNLND
                     YKNISKYIESCIKNEILSISNVEIKNILKE"
     gene            complement(47157..48338)
                     /locus_tag="PC545_001073"
     CDS             complement(47157..48338)
                     /locus_tag="PC545_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDF2455861.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKIVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGDVFNRVLNKNEAVKIMTGGKIPQGANC
                     VIKKEDVTVIGDMITLNIKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVKNMLQSVDFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVGTLGEKLLFGLSGNPHAAIVVFDNVIVPVIEYLTYKKGTHHIKAIFKGEFNKFSN
                     KDRFVSGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDVVI
                     R"
     gene            complement(48549..49283)
                     /locus_tag="PC545_001074"
     CDS             complement(48549..49283)
                     /locus_tag="PC545_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455862.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINENKVVNVETNSFSSQGISVIDAESSIVIEKSDHEKSAVSLENFR
                     HIDLGEYVGVEDSRINEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
BASE COUNT        15491 a         9211 c         5075 g        19591 t
ORIGIN      
        1 aattttaaaa ataaattttt gttttattaa tgttgtcata acaacattta cttgattgaa
       61 atagtattac ctataagttt aaatatatat aataatagac ttgattctat ttcatattat
      121 taaaatagag taatagttaa attctactac tctatctata atctatttcc taattattta
      181 atctcaaatt tagctcttca tcatttaaat ctttcataag atttctacag gtactaggtt
      241 tattccaaca gaaagactag ggcattaaac acttatatat ctgtttcttt agactgtaat
      301 attaatattt tttaaatacg ttgacttttg ctttttctaa cttctctcca tgatagttct
      361 cctctatcaa gagcttttat aaatatttct gctgaagcta gatttgtagc tattggaaca
      421 tgataaacat cacaaagtct tattaacgct tgtatatctg tttcatgagc ctgagatgtc
      481 aatgggtctc ttaaaaaaat aactaaatct atttcttgtg ttactattaa agaacctatt
      541 tgttggtctc cacctaaagg accagatgct aatctattta tatttaattc agtttcatcc
      601 attattcttt ttcctgtagt tccagttgca tatagtccat acttttttaa aattgattca
      661 taacctatac aaaatccgac catagtgttt ttcatttggt catgtgctac taatgctata
      721 ttcatatatc taatctcctc aatttatgtt tagatgtatt taaatttaga aatttatttt
      781 ctttcttctc atacacacat ttagtactaa tcctaagttt gctaaagatg tcaataacga
      841 acttcctcca taacttacaa aaggtaatgt tactccagtt actggtatta acgcaaccgt
      901 catacctata ttttggatta tctggtaacc aaacattccc ataacaccaa ccactatgag
      961 ggttccataa aaatcttttg catctcttgc aattgctaaa agccttaata ggaatatcat
     1021 aaataagatt atcaacactg ccattcctat tacaccaagt tcttcaccta caacagcaaa
     1081 tataaaatca ctatcttgta caggtaaaaa atcttcttga ttttgacttc cattgtaaag
     1141 tcctttacca gttacgcctc cagaaccaat tgcaatcaga gattgcataa cttgatagtt
     1201 tccttttaga gttacatctt ctggatttag aaaagcttct attcttacct tttgatggtc
     1261 tgccatcatt aaatacatca gtggcattga cactaatagt atgattattc ctctttttat
     1321 caactttgag ctaagacctg ctgtgaacag cattgcaaat atcatacaac aaaatacaat
     1381 tcctgttccc aagtctggtt gtgcaatcaa taatccgata aatggaagag aataaactac
     1441 aacaggcata acttccttta acgtatttaa tttatccttt ttggattcaa gtatctttgc
     1501 ataacttagt acaaatgtta atttgactat ttcagatgtc tgcaagtcaa gtggtcctaa
     1561 gttaatccac gacctagctc caccttttac tgtcccaatt ccaggtaata aaactatggc
     1621 taacaaaata agacttatta tataaagcgc tttataatac ctgcctagaa ggttatagtc
     1681 aaaaaataat atgactataa tcattcctat cccaagtaca aaagctaatc cttgtttaat
     1741 caattgatta tatgaacctg ttgaattagc atgtgttgca ctactaagta taacaagccc
     1801 aaaaataaag atagctaata ctgtaactat aagtttccag tctaactgtt ttatcaactt
     1861 aattgtcgac ttataattaa tattgatttt tttcaaagaa tcaccgtctt tctatcttta
     1921 aacatataat taaataaaag gggcatatac caataaggat atcccccttt taagcactaa
     1981 ttacatacat ctatctttta tattctttag tggtatatta gcaactaatg cagatacagg
     2041 tttgtcatca tgctcacctc tgcatttaga catttttatt tcaagaccat catcttctat
     2101 ttctgcataa ttagctatta ctttcaaaat gtctgttttt atcatctcta aaagttgtgg
     2161 tgaacaatct actctatcat gaactaaaac tagctttaac ctctctttag caacagattt
     2221 actagtttta gcctcgttag aaaaaactct aaataaatct aacacagatg tatcctcctt
     2281 tgctacttag ccatgctaaa cattttttta agtctaccaa agaatccatc ttcaacttca
     2341 agatcaagta gaggaacttc ttcatttagt atcctttttg cgatattttt gtatgcttga
     2401 ccagcaagtg acttagaatc aagtatagct ggctctcctt tatttgttga tataattatg
     2461 ctttcatcat caggaacaag acctaacaaa tctattgcta atatttctat tatatcttgt
     2521 ttatccatca tgtctccacg cttaaccata tcatttctaa ttctatttat aactaatctt
     2581 atctctttta tttcatttgc ttctaataga cctattattc tatcagcatc tcttactgct
     2641 gatatttctg gattagtaac tacaatagct ctatctgctc cagctactgc atttttaaag
     2701 ccttgctcaa tacctgctgg acagtctatt attatatact cgaaagattc ttttagtttc
     2761 tcacacaggt caatcatctg ctcaactgag acagcatttt tatctctagt ttgtgctgct
     2821 ggtaataaat atagattatc aaatcttttg tcttttatta gagcttgctt aagcctacaa
     2881 gttccttcaa ctacatctac tatgtcataa actattcgat tttcaagacc cattactaca
     2941 tctaagtttc taagtccaat atctgcatcc acaactactg tttttttatt ttctaggctt
     3001 agcgcagttc ctaagtttgc tgcggtagta gtttttccaa ccccaccttt accagatgtt
     3061 ataactataa cttcgctcat aatactaccc cctatttttc tttctatttg tctaactttg
     3121 acaaatagct ttcgattaca attcttccat tacttacaaa ggctatttct gggctaattt
     3181 ctgattcact ttcataattt tcttcatcat ctggagcttc tgcaatatta tctgctattt
     3241 gtaatatttt aggattaaga tttccagcaa caacatatgc aaattcattg ccttctgcac
     3301 ctgcatgtac aaaaccttct attttaccca ttacaaaaac attacctcct gctacaacct
     3361 tagcaccagc atttatatct cccataacaa ctgtatttga ttttgaaagg acttttgaac
     3421 caggtttcat atcattcaat ataactacat caccttcaaa ttcaatattt tcaccagatc
     3481 ttagggtatt gacatatttt gttggaaaat tactattttc ttcaattttt tgatcttcaa
     3541 caaattccac atcaaatcta gaagtaattc cttctttaag ttctagaatc tccatgtcag
     3601 ttaaacaatc actattaatt ttactaattt tagccccatt aaaaaagcca acatatgctt
     3661 ccaatttgtt tattattttt tcctgtattt cctcaaatgg agcttctctt tttatattta
     3721 ctattatccc tcttttatta cccttgaact ctactagttc ttgactacaa atttctctta
     3781 aagacataac aacctccaga gtgatatatc atttatattc ttcaaatacg ctttaaaccc
     3841 ttttattttt tctatataaa gctaaagttt atacatagat tgagctatct attaatttgt
     3901 gactcgaaat ttatattttc gtcagaagaa ttgttaacat cattcgtctt gttgtcattt
     3961 ttactattag ttggctctaa tcccatataa gttccaataa cttctctagt tggtaaaagt
     4021 gagaatacac ttgaatcacc ttgaggaatt acacatacaa cagctatttc tgggtcatct
     4081 gctggagcaa aagcaacagt ccacgtaaaa gaaccataat cttgtttaaa tctatctatt
     4141 tggtcatctg ttatttttgg atttaattct tttatagctt ttcttagata tgaagaattc
     4201 accttatcag tgtcctctaa tctaactttt actccatctt ctaattcttc ctctaatttc
     4261 tttctttctt cgtctttcaa atctttattt tctaatttct tttttatttc cttttcctta
     4321 gctaatgaaa gctctttttc tttttctgct ttcatcttat cagctagttt aatcgcatca
     4381 tttaggttga cattataaga tgacatatga ctttttaaat attcatactc gttatctgta
     4441 ggtattttcc cacttttctc agctgtacca gtttttgctg ctacatctat tggaaaatca
     4501 gcaaaagcac tcttcgctgt accttgtctt gcaaccaatt tcataccttt tgttaattct
     4561 tttaagttat ctggattttt aaaaggtatt ttttcgactt tcttctggtc attaatttta
     4621 actgatgaat aatcacttga aacagctcta tcaactacag ataattctac taaattacct
     4681 ccatttgcta tagcagctac atatcttgat atttgagctg gtgtataagc attttcacct
     4741 tgacctatag ctaagttaaa tgtatctgca gtactccatt ttgcaaagtt aaaataacta
     4801 aatactgcta aatctgctac ttcttcaatt ctatcttctt taacatccaa tttttttagc
     4861 ctattcattg tttcaactct accaggagtt ttcttttctg ctgcccagct tacaatctca
     4921 ttgattcttt tctcatattc tttaggattt ttttctctag ttatatcaac aaaatcattt
     4981 gccatttctc tttctaaatt tgactttaga agagcttgtg ttgatttcaa tttatcttct
     5041 gtgctaggaa ctttacctgc tctttcttct acttcatctt gaagccctgt attttggtca
     5101 agcccaaata attttgcata ctcaagtatt ttatttgcat tcatatctat accaatgctt
     5161 tttccatctg gccatgtttt acctgtacca attgttgcca tatatatatt acaagactct
     5221 tgtattgctt tatataagtt tgtcatacca tggtttcctc taccatggtt ccatacatag
     5281 tcaccaaaag attttttacc taacattata accccagggt catttatagt aaagtttgga
     5341 tccagaccat tttctaacgc tgccattgaa gttagcatct taaatgaaga acctggctga
     5401 aattcccctt gtgtaaccaa gtttaaaagt ggacttcctg ctagtaagtc atttggattt
     5461 tttggttgaa gtgctttata atcctctgta gaaatacctt ttgcaaattt atttgggtca
     5521 taattaggat aacttgatga agctaatact tctccatttt taacatcaat agctataaga
     5581 gctgctgact gtgcttttcc tgcatatgca ctaattggtt tgtccccaaa tttactttta
     5641 aaagtacctc ctttagaagc aacttcgatt atctgtttta atgcattgtc tgaaacttct
     5701 tgtaaatctt tatctagtgt aagatacaca gtatctcctg atttaggttt tttagattca
     5761 atttctttac tgattctacc caatgcatca actttaacca ttttataacc atcagttcct
     5821 cttaatctac tttcattact cttttctact cctgcaagac ctaccatgtc tccagtttca
     5881 taaccttttt gaaggtatga ttctatttga gtggatggca tttttcctac atatcctaac
     5941 atatgagaag ctaatgaccc attaggataa tatcttactg gttctactgc tattgaaaca
     6001 ccaacaaggt ccatagcact ttcttcaatt tgagcaatcg tagtttctcc tacatcctta
     6061 gcaatagtta ctggattata ttgagaatat ccttttgatt ttatcaaatc ccttacgacc
     6121 attatttttc ttgcatcttc atcactcaaa cttttatcaa tttctaatgc atcactattt
     6181 cttacttttt caaacgcttc tttggcactc agtttagttt cttttatctt ataactagcc
     6241 aaccaatctc tcttatctct ttctgctgtg aacatccatt tactaactaa aataggtgga
     6301 taatatccat tttcatttag ttttgcctgt aatcttgtcg catccaatct tttatcttct
     6361 tgagataata ttccagctga tataagttta tccaccaagt aataaaaact ttccttagca
     6421 ttataatcat ttggaatacc attttctgat ttatattccc taatatctct gtcatatgta
     6481 taatagtact ttccattttc tacatatata ggaaactcat ctacatattc ttcaccattt
     6541 ctctccagca aatttataag ttttaatgat atttcatttg ctcttgaatg cttatttgca
     6601 tctttcttat taatatcgtt acctgaaact tgaacagtaa ataagttttt atttcctgcc
     6661 aacagtcttc catatctatc ttttatttct cctcttggag cttctattgc caacttttta
     6721 tatgttttat tctcagctaa ctcattgtaa tattcatatt taaatgtagt catatagatt
     6781 atttttacta aaatagctaa aaatatcacg cccatgacta tcttaattat ttttagtcta
     6841 tcaacttgtt tattttcttc catgaaatca ccaactaatc ttctttaagt tttaaaactg
     6901 ctcttttaga taagcgatat attactaaac caattaaact attcactatt ggtgctacta
     6961 gtagaccttt tacaaaaagt gttgatatgt tgtagctttg ataaattata ctagatgtac
     7021 ctatattgac aattgaatca attaaactgc ctataaaaat taaaacgaaa gtaataatat
     7081 aactaccttt atatagctga tctctcatgt atccaattgc atatgcaata ataaacaaaa
     7141 ttaatgcatt tacaccaaaa attccaccaa cggttatgtc tttaataagc cctattattg
     7201 ctgctataat cccaccttct aattcatcta aataaagaga gattatggtt acatatataa
     7261 gaactaaatt aaagcttatg ccaaaaatat ctatataatt tgttatacta ttttctatag
     7321 tcataagcac aatccccaac agacaaagta aaactttttt cattgttttt atccccttta
     7381 tcctatattt cttggttcta tgactacaac gtcatttata ttcttaaaat caacataagg
     7441 ttttacaact acatatttta atgacttatc tttatcatca accacttttt caacttcgcc
     7501 aattggtata ccatctggga agaatccaag acctgatgtt gttattatat ctccttgaat
     7561 tacattatat gatgagtcaa acatatatcc ttgcaaatat cctttactat tataactatc
     7621 tttattatct aaagtagtat tttgagtaat tgtaccttta gcattggcat cctttgctag
     7681 tttaaagctt acagatgcct tagaatccaa tagtgaaatt gccttactat aattgttgga
     7741 aacctcataa actatgccaa ctaatccact accattcata actatacttt ctttttttac
     7801 acctgaattt tttcctgcac ctatgacaaa actctcatac cagtttccat catttttacc
     7861 aacaacactt gctgaaatac ttgtcgcctt atactcttct tgtacaaagt tcagtgtctt
     7921 ttttaactct tctagcgatt ctgttttatc tagtttggca tttaaagcta tcatattttt
     7981 tttaagtttt tcattttctt cttctaattt ttttaccttt ttagaattat ctttaaaatt
     8041 tgctacatct ttaaaaccat ttttgataaa agaaaatcca tcatttattc ccttacctac
     8101 acttgtaata gcatctgaag ctactcctaa atttactgga ctaccactag aaaatttacc
     8161 aattgatata cccacaattc caattaaagt gatagcaact acgcccgttg ctatcacttt
     8221 aacattaatc ctttttttct catttttgtt cttgtcaaat ctcaaggcca tcaccaactt
     8281 ctaattctta gtattcatca ttaatacttt ttcaaagatt tcttggtctt caacagattt
     8341 acctgttcca agtgcaacac aatcaagagg tgtttctgct atttgtacag acataccagt
     8401 ttcctgtgta ataagcttat caagacctct aagcaaagcg cctcctcctg ttagcattat
     8461 tccgttttcc attatatctg aggctaattc tggtggagtt ctttctagtg tagattttat
     8521 tgcatctact attgaactta caggttcttt taatgcttct ctaacttctg tagagcatat
     8581 ttctattgtt ttaggtaatc ctgagattaa atctctacct cttatttgca tatttatttc
     8641 ttcatcatct ttaaatgtag aacctatgtt aatttttaca ttttcagcag ttctttcacc
     8701 tatcataaga ttatattctt tttttacata agccactata gactcgtcaa attcgtctcc
     8761 acctattctt atagatttag cagtaacgat tcctcctaaa gatattacag ctatttctga
     8821 agtacctcca ccaatatcaa ctaccatatt accttctggt tcttcaactt ttaatccagc
     8881 ccctatagcc gctgccattg gctcttctat aaggaatgca tctttagctc ctgcttgtct
     8941 tgcagcctct tctatagctc tcttttctac ttctgtaact ccaaaaggaa cacatatagc
     9001 tattcttgga cttacaacac cttttttatc agccgctttt tgtataaaat agctaatcat
     9061 tgattgagtt atgtcaaaat cagctattac tccatctttc ataggtctta tagctactat
     9121 atttcctggt gttctaccta tcattttttt ggcttcttca cctactgcta atacctcttt
     9181 actatcatct cttattgcga caactgatgg ttctctaaca acaatacctt gtccttttat
     9241 gtaaactaat gtatttgcag tccctaaatc tattcccata tcctttgtca tcttattaaa
     9301 actaaaaaaa cctttttttt ctttcttttt ttccttagcc atgattttac tccttcctac
     9361 tattaagaat catgtttaaa acattctcct attattttat cacatataaa gtcaaattat
     9421 catattttct ttaaagctaa agtataatcc atctccaata attatatggt ctattatttc
     9481 tattccaatt atttctccac atttaataag tcttgatgtg atatttttat cttctcttga
     9541 tggttctaca cttcctgaag ggtgattatg catcactatt atcttacttg cacttcttct
     9601 tatagcttct ttaaaaacct ctcttggatg gactaatgac gagcttaaag tccctataga
     9661 tacatctaca tcagatatta tctcattttt tgtgtttaaa agaaccgctt taaaaacctc
     9721 tttttttaaa taccttaggc tatccatata atacctctgt atatcccaag gattccttat
     9781 tttatacttt ataggtttaa aactagctac tctagagcct agttctaaag ctgctttaat
     9841 ttgggttgat tttgataacc caattccatc tatattacac agctcctcta ttgtcatatc
     9901 ttctaaatgt ctaataccct gaatatctct atttattata tagttggcta attcaatagc
     9961 atttttattc ttgttgccag ttcttaaaag tatagccaac aactcagcat ttgataagct
    10021 ttttacgcct ttggcaagca tcttttctct gggtctttcc tctggtgcca tttcttttac
    10081 cttaatggtt gaattaaatg actttttcag ataaatcaca ctccataaaa aagatttata
    10141 ctaaaatatt tttttaatag gtcgcctaac cttgaaattg gaagaccaac tatattaaaa
    10201 taatctcctc taatttcttc aactaacaaa gctccataac cctgtatacc ataggctcct
    10261 gctttgtcaa atgactcatt agttttcaga taatcctcaa tatcctgctc tgataacgtt
    10321 ttaaatttca cattgctgat tacatagtca attacctttt tatcttcgca caagtttatt
    10381 aagctaatac ctgttatgac ttgatgctct cttccagata gtctttttaa catatctctt
    10441 gcacaagact catcttttgg ttttcctaaa atagcattgt ctaaaacaac aactgtatca
    10501 gctccaataa caacatcttc attatgttca gatgctacag acatactttt ttcaaaagct
    10561 aatctcataa ctaaatgttt tggtgtttca ccttccaata taatctcatc aatttcattt
    10621 ttaattatat caaatcttac attcgtattc tctagaattt ctttccttct tggagaggct
    10681 gatgccaaga ttatattcat ttattcacct catttgtacg taatttgtac ctatttagtc
    10741 tatcactata ttgtaaaaaa ttcaacagaa atgtcgaacc ttttttgtta cactattgtt
    10801 atatttatta ccattagaat aaatatacat aaaatagagg tatggaaatt attccatacc
    10861 tctgttttat gtactcatta gagatatgga aattattcca tatccttatt ttgtccatat
    10921 tacaaaattt taatagcttt ctttggacat ttttccatac ataaatggca tcctacacat
    10981 ttatctgcat ctaccttatg tttttctttt aattctccct ctattgcatc aaacttacat
    11041 tgttttttgc acactgtaca tccaacacaa agttcttggt caatagtaac tttctttttc
    11101 ttagttatgt cacctgatat tacttttgta gggcattttg ctacacattg catacaacct
    11161 acacacttat tagggtctat cttagctatt ttgtcttcaa atattattgc atcaaattta
    11221 caagctttta cacacattcc acagccaata catccagcag tacatttttc tttaacagct
    11281 ttaccaaact ctttgctatt acactctaca actacttctt gactttctgg ttttgtgatt
    11341 attataccct taggacaaac ttctttacat tttccacaat ttacacactt ttcttcatct
    11401 attactgcta ttccatcaac tatagaaata gcatcaaatt tacaagcatc tttacatgtt
    11461 ccaagaccta aacatccaaa tttacacatc ttagcacctg agtttaaaac atttgctgct
    11521 ctacaatctg atattccttc atactcatat ttatcttttg cacttgaaca agttccttta
    11581 caaataactt ttgctacttg tttctcacct gcttcagcac ttattcccat tatttctcca
    11641 acttttgcac caacatctgc tccacctaca gggcaaccat taactggtgc attgccttca
    11701 actatcgcat ttgcaagacc tccacaacct ggaaatccac aacctccaca gttggctcct
    11761 ggaagtactc ctagtatagc ttccactctc tcgtctactt ctacagcaaa ctttttagat
    11821 gcaaaatcta gtactatccc gaagattaag cccataattc ctaaaactaa tacagctgta
    11881 agtatcacca ttttcacccc tcttttttat agctttataa gtcctgtaaa tcctaagaaa
    11941 gctattgaca ttaaacctgc tgatataagc gttataggga aacctttaaa tgcttctggt
    12001 atatcagcta attctagtct ttctcttata cctgcgaata ttactattgc taaagtaaat
    12061 ccagctccgg ctccaaatcc gtttattatt gtttctacta aattataact tttttgaaca
    12121 ttaactagag ctataccaag aactgcacag tttgtagtta taagaggtaa ataaactcct
    12181 aatgcttgat ataaagatgg actcatcttt tgaattacca tttcaacgaa ctgaactata
    12241 gaagctataa ccaatataaa agctattgtt tgtaagaatc cagttccagt ttttactaat
    12301 aaaatctgta taaaataagt tataattgaa gctagagtta aaacgaacgt aactgcaact
    12361 cccatcccta ctgctgtatc aaccttctta gaaactccca agaaaggaca tatacccaag
    12421 aattgagatg ttataacgtt attaacaaga acgatactta aaaataaaag tattaaattc
    12481 atttattaca cctccactat gcttttttac ttcttaattt attaaatcct gcaaataaga
    12541 atcctaatgt taagaatgca cctggtggta atataaatat taatactggt tggaaagatg
    12601 ctccaaataa tgtcattcca aataaactac catttccaag taattctctt actgcaccta
    12661 ttattgtaag tgcaacagta aatccaagac cttgacctac accatcaact attgaaggca
    12721 tagctcctgt tttgaaagca aaactttctg cacgagctaa tattaaacag ttaacaacta
    12781 ttaatggtat atatatacca agagccttat caagagcagg aacatatgct tttaaaagca
    12841 tacctactat tgtaacaaat gtagctataa ctactataaa tataggtatt cttatcttat
    12901 ctggtgtaat ttttcttatt aaagatataa ctaaatttgc acatattaaa actgctgctg
    12961 ttgaaagacc cataccaatt ccatttattg ctgaagttgt aacggctagt gttggacaca
    13021 tacctataac ttgaacaaat gttgggttct catctattag cccattttta aatactttag
    13081 ctaaattcat aattaaccct ccttatttat taagagcact attgaaaaca tctatagcag
    13141 catttactcc tgtagtaact gctgtagatg taatagttgc acctgatatt gctaatattt
    13201 gattatctgc tgttgtactt ccttttgcta cttcaagagg ctcagcagtt tttcctttgt
    13261 attgaccctt aaatgcatca tccttagctt ttgctccaag acctgctgtt tctgacatat
    13321 taccaatgtc aacacctgta acttgacctt cagatgatat acctaccata acttcaattt
    13381 ctccaccata tccacttggt ttagctttta aagtatatcc aaccacttca gaaccatcta
    13441 aaccttcata cacttcagct ataagaccat ctccaaggcc tttgtaagta tcttccttca
    13501 tttctttaaa ttcttttgct tctggaagga ctgcttttct aagttcatta tttgcttgta
    13561 tatttctttg ttctattact ggagctgtaa cctgattagt agctccaaga gctaatgcag
    13621 ctattgcact tatagcaagt aaagtaccac ctaatctcac catactgttc attatttagc
    13681 cacctcccca aatactctat ttctaacaaa cttatctata agtggagcaa gacagttcac
    13741 taatagtatt gagtaagata caccttctgg atatccacca tacattctta taacagttgt
    13801 tataagacca gctaatacag cgtatattat ttgacctttt ttagtcatag gcgaagttgt
    13861 ataatccgta agcatgaaga atccacctaa cataagtcct ccagcaaaca gttgatatat
    13921 tgcgaaagtt atattaaatc cacctaaaag gaatgttaat ataaatacag ttgctatgta
    13981 aaatactggt ataacataat ttattatgcc tttatacatt aggtatactc cacctaataa
    14041 taataatatt gctgatgtct cacctataca tccaccaata ttccctatca tcatgtcagc
    14101 aagactaatt ccattgctag aaatatctgc cagtcctgct gctccatttt tcaaaaagct
    14161 taatggagtt gctgtagtaa ctgcatctaa atttttacct atccagttaa cacctggttg
    14221 agtccaagca gtcattgcta ctgggaatga tgctaataaa aatgctcttg cagcaagtgc
    14281 tggattgaca aagttacatc caattccacc aaataccatt ttaactacta atattgcaaa
    14341 tattcctcct actagacaca tccaccaagg aagtgatgct ggtacgttaa atgctaataa
    14401 taatcctgtt acaacagctg aaaaatctcc tatagtactt ttttgcttcg taaatttttg
    14461 atataaaaat tcagtaccta ctgtaccaag tatacaaaaa aacatagcac tcaaagcatt
    14521 aagtctaaag aagtatactc ctgctactgc tgctggaagg agtgctataa taacttgttt
    14581 cattatatat gaagtatctt cattacttct cacatgagga gaagatgata ctatcaactt
    14641 attttccatc gtttttcccc cttaactttc tttatttctg agcagcttgc tttcttctct
    14701 ttgctcctat ttctctcttt gctgatctta tagattgaag taatggtctt cttgctggac
    14761 aaataaatga gcaagaccca cattctatac aatccattgc tctatgatat tcagctgtgt
    14821 catagtcatc ttttaaagaa tatgcactta tgaaaagtgg ttgtaagaat gatggacata
    14881 catctgtaca tcttccacat cttaaacagt tttgaatatc tggagtacgt gattcttcct
    14941 catccaaaca taaaattcct gaagaacctt tgttagtcgc tatttcagta gtatattgag
    15001 ctattcccat cataggtcca cccattataa ccttaccaat cttcttatct tctttaaatc
    15061 caccacattg ttctattatt tctgaaacta atgttccaac ttttgttatt aggttctttg
    15121 gctctttgat gcaactacct gttattgtag tgattctttc aaccaaaggc atacctgttt
    15181 taatagactt agctatctgt gctgctgtgg caacgttgtc aacaactgca cctgctgcta
    15241 ttggtaatcc accagatgga acttctttgc ctgtacacgc ataaataagt tgtttctctg
    15301 ctccttgtgg atatttcact tgaaggccaa caacttcgat ttcgctatag tctttagcta
    15361 cattttgaat tgcttctata gcatctggtt tattcgtttc aattcctata aatccttttt
    15421 tgacatctaa taccttcatt aatgctctta aaccaaacac aacatcttct ggattttcaa
    15481 ccattaatct gtggtctgca gttaagtatg gttcacattc tgcaccattt aatataacta
    15541 cttctgcctt gctatctggt ggaggagata cttttacatg gttagggaat gtagctccac
    15601 ccatacctac tattcctgcc tcttttatga tacctactat atcttctttg ctcaaatcct
    15661 ctaactgacc ttttggttga acactctcat gtaattcttc cttaaagtca ttttcgataa
    15721 ctacacattg agctgaacct cctggtactt catgttgttc aatagcaata actttacctg
    15781 atactgaaga atgaacgtta catgaaacaa aaccttgttg ttcacctatt ttttgcccaa
    15841 actttacttc atcaccaact tcaacaatag gtttagctgg tgcccctata tgttgctgaa
    15901 gaggaatgta aactatcttt ggagcttgag ctttttcaag tgcctttgtg ttagagtact
    15961 cttttctata tggaggatgt atccctccct taaaagttaa gagtttcatt gttttacccc
    16021 cttatctata aaaaaacatt ttttctgcct tctataagta tactatatat attaaaaaat
    16081 cttaacaatt gttaaaaaaa tatccgtatt tttataattt attttataaa tcaacacctc
    16141 tatattaagt caaattgagg aaaaggattg cttaattaaa caatcctttt catatagctt
    16201 tctaaagtcc aaattttctg tattttattt tatagaattt ataaacatag agtactcctg
    16261 catggatgat aaaagcagac ttttacttac actatatttt tcttcatttg ctgcttttga
    16321 agctgtatca attttttcat tcacattctt tacatactct attaaattat ctttaaataa
    16381 acgcatttca gaataatcaa tcttttcagc ttcttttgat atattttgtg atattgcctt
    16441 tctatttgtc aaaatagtat tatatttttc catatctaca ttattttcag cattcgacca
    16501 aaaacttgat tcttctttaa agtttgttat taatttattt aattctttag atatactctc
    16561 tatataagcg tagttacttg tatactctaa agacaacata ggtgcatcta agtgtgataa
    16621 aaatgcatct ggaaacacct ttcttacact gcttatctgc tttctaacat cattttcatc
    16681 aaaagaggaa taggtctgta ttttttttac tccatccttc tccataactg aaaaaggtat
    16741 gtcattatta actaatgatg tctgtatttt tttcaaatca tcattattat ctattgcagc
    16801 aacttgaata gtatacatat cccagccttc tatagtagct aatttgactt cttctggctc
    16861 tttagtgtta tttaccgctt tttctggatt agtttcttgt ttatcatctg aattggtttg
    16921 tgatttagat tttttccctt tttttattga atttaattca tcagatatat ctttataagt
    16981 tatatctgat gaattattta gaaatggtat tttagccgat acatactcca atatcttgct
    17041 atcttttatt tttgtataag aataatagcc aattagacac aagcaagcaa tcgttataac
    17101 atatttacgt aaatcaatct tatgtctatt attattaaat cctctgtata tcgtttttct
    17161 tctattcatt agtttgtccc tcccaaaatt atattattaa taaagtatat ttttaagagt
    17221 cacaatttat gaatataaat aattatttct tctaattttt taataagaaa ttgtacttac
    17281 taaacaaatc ttcttgcacc aagtaatact ttagaccaat atgaactagt taaattatcc
    17341 actttaacct tattaggatt agtctgagta ccactacaat ggataaattt actatttcct
    17401 acgtatatac ctacatgatt agttgttcca tcaccatcag tgtcaaaata aactaaatct
    17461 cctggtgcat aatttcccat agaaattgca cttccaaaat tagcctgttc tctagaaact
    17521 ctaggaatag aaacgcctac agaatgttta aaaacatact gtgtaaatcc agaacagtca
    17581 aatttatcag gtcctgaagc tccccattga tatggtattc caataagagt atatgcaaaa
    17641 tctacgattg ttgaaccttt tgctgtagat ttatctgaat ttccagtaaa tgatggcttt
    17701 gagttactat tagattgaga attttggtta gatgatgaat ttggtttttc ttttgtatcc
    17761 tcattagtct cagaaatata actggcactc acccatccaa ttgttccact cgataacttt
    17821 accttatacc aaccattact tttagctttt aattcaacaa catctccacc atttaatttt
    17881 cctattatag aatagcttgt cccaggacca cttcttacat ttaaactcac agcagatgtt
    17941 accttaccat tcttgccttc aattgattct tcgctagatg gttttttatc agaattatta
    18001 gtactatttg aattactaga attgttcgtt ccaacatctt ctgatgtttt agaaatatat
    18061 tggctactag cccatccagt aactccattt gatagtttta ttttatacca tccattgctt
    18121 tgttctagta actttactac atctccatta tttgctttac caactaatga atagttagta
    18181 cctgcaccac ttcttacatt cagtctagaa ctcacattta ctttaccatt tccactaata
    18241 gttgtcccac ttgaagttgg attattttga cttgatgtac cttcagacga accacttgtt
    18301 gatatgtatt ttgcacttcc ccatccaaca acagaactcg atacccttac tttataccat
    18361 ccattagact tttctaaaat ctctacagtt tttcccttgt ataattttgc taccttatcg
    18421 caatcagaac ttggtccact tcttatattc aaggcacttg ctgttactgt tcccttttca
    18481 agagcacttg catttatgga gctaacagat actgctactg ccccaattcc taaagctgct
    18541 atagcttttt tcactactat cactcccccc aacaaatttt tatagtcata aattcataac
    18601 attattatcc atttttagtc cctacaattt aatgataact tattttctta ttaattgtaa
    18661 agaaattata aacatatcct taaaattagt gatttttagg aagtatttca atccaataat
    18721 tatctgggtc cgctataaaa taaattccca tactttcatt ttcaaaacat atgcaatcta
    18781 aatctttatg aagttttttt gcctcatcaa actcctcaac ttctaaagcc agatgaaatt
    18841 cattgtctcc taaattatat ggtctatccc aatctctaag ccaagttaat tctaatgtat
    18901 gactagtaat gccatctcct aaataaacta atataaaact tccatcttct gcttccttgc
    18961 gctttacctc tttaagccct aaagcttttt tataaaaatc taaacttttt tctaaattag
    19021 taacattgaa attgttatga caaaaattaa acttcatttt gaaatcactc tccaattttt
    19081 atttttttta tattatatca ttattataaa tttatttaaa tcatattaag ttaaaaattt
    19141 gaatgatgat gaccaattac caaatcatgg taaattattt aaaagaatat gtcaaaaaaa
    19201 gaataagctc tttcaaatgc ataaaaaatt tatacccaag ttatacttaa tcatgcaaat
    19261 tggcagaact tattctttaa aactctaact tttaagtttt tctttaataa tataatttat
    19321 tacttttttt ctaatatctt cagcattata atcatcttct acttctatat ccaaaagttt
    19381 tgctattgac ctcatatctt ctctagtctt tgacctatag cttgatatta attcatcttt
    19441 tattttctct ggttctaaaa gatattcttc atctcctctt ttgaaaacaa cgtatttttg
    19501 agcatatgaa cttctattgt agtatatata tcttgatatt tttcttaaaa tttgattata
    19561 tgaaagtcgc ttgttaatat caagttttaa gtactctgaa aaagcaaata gctctttttt
    19621 tgatttaaat aatgcttctt tctttatagt tcttgatatt gaagttacac tttcttgata
    19681 tgcacacttt tttaacattt ttaaatatac tctattaaga ccaatacttt gattgaattt
    19741 ttttatatta ttattaataa tcttatccaa gttaccttca aagtattttt ttgctccttt
    19801 ttgtgataca taactctcaa gttctttgtg ggccatatca agtatcattt tttttaaatc
    19861 ctctttatat cccctagatt ccatgatact ccctccctta gctatttata gtatattcaa
    19921 aaagctcaag aaatgtgagt cattacaaaa tttttttact ttttatgtat atattaattt
    19981 aaaaggggag ataatagtaa tgtaaaatca aacatactca atctccccca tttttagacc
    20041 tctattactc ccccaataga ggtctttttt tagtttatca atttctattc aattcccttt
    20101 agttcaaatc ctaattctgc tatcacttct tttaattttt caatagatac atcatctttc
    20161 atatctacag atgcacactt attttctaaa tctacatcta aaacttcaat accatctata
    20221 tcttctgtta atgcagtttt aagatggttt acacaatgac cacaactcat accttctact
    20281 aatagtttct tcttcattta attacctcct tataccctat aagggtattc tatcatatag
    20341 tttatacttt ttaaatagaa attaatcatt taatttgatt cattcgattt tatatctaaa
    20401 ttagatattc cctcatatac atcaaaatca tttggagtaa ttttaccttt taatctacta
    20461 ttacttacaa tatagttgtt tctcacagct aaacttataa atgttgcatt atcttttata
    20521 tatgtctgta catctttgta tgcttttttt gtttcaattt cagacgtaga acttgtaagt
    20581 gcagacatat atccatttaa cttatcatca gtatatccac tactcgctat aatactactt
    20641 gcatctggta taattgatag tttccatcca actaatgcta aatcataatt tttagaatta
    20701 agagcatttt ccatatcctt atctgacagc tgatttactg tactatttat tcctatagct
    20761 tttaagtttt catttattat gtttgcagtt tttattctct cactattatc tctgtttact
    20821 attatcttta agttcatttc agaaagactt ttctttactt cctctttttc tctatttttt
    20881 ttctcttcag cttctttttt gattctatct tcttctgctt ttttagcttt tgcttcttct
    20941 tctggcgtaa gcttctttga gttcttttta gctgattttt tctgattttt tactttattg
    21001 tcattttgat ttactgagtt tacatttgca tattctacct tagcaagaca ctctatcgcc
    21061 ttatctttat taaactctaa atccttcatt tcactattat agtattttga ttttgaatta
    21121 agtggaaaat taactggagt agcatcatcc atataacctt cactaataat tctatcttta
    21181 tctatagagt gtgcaattgc ttttctaaaa ttaacatctt tgaaaaaagg attattataa
    21241 ttaaataata cacactcata atctcttcct tgatattttg ttatattaaa ctctttttct
    21301 tgaaacttac ttaaatcatt taatgtaaca ttggttatat cactatcaag agccataacc
    21361 atagatgtcc ttgcatcttc attaggcacc atacctactt ttatgttctt catggttttt
    21421 ggaacttctt catagtaatc tttgtttaca gataaaacca ttccttctct ttctgtatat
    21481 gattctattt tatatttccc atttcctatc atattattat tcttgtcatt tacatctttt
    21541 gaatctaaat gattttgaga tacaattgga aaaattaaac tatcaattga aaaagcatat
    21601 ctagctttaa attttattgt aaaatcttta tctgatttaa tattcacaga agctatattt
    21661 ttagtaaatt cattatatgg actatctgca cttttttgaa ttaaaccaat agtatagttt
    21721 acatcatttg aagtaatatt ttttccatca tgccactttg catcttttag ctttatatct
    21781 atactcattc catcctgagc tatattatac tccttaacta attgaggaat tacattgtag
    21841 ttctcatcta tagtaaataa actatcataa actaaattca ttatataacc aacagactta
    21901 tcagtattag ttataggatt tatagttttt ggagttacca ttgttaagtt tatatactta
    21961 gcatctatag aactttgact ttgtaaactt gaatcactac tactttgttt tgttttagta
    22021 ttactacaac cagctaccat aagagttata gctaaaacga ctgttaagac ttttattctc
    22081 ttcattagtt ttttcctttc ttgccatata tcttattata atgttcgtaa ttatttttta
    22141 ctttttctac atattttgaa gtttgcttaa aaggtatatc atgtagattt tcgccatctt
    22201 tactatattc attattttct aaccattttt tcacattacc tgaaccacca ttatatgcag
    22261 ctatcactaa atctaattta ccaaattctt tgtataactt acttaaatac caacagccaa
    22321 gtcttatatt agtctctggg tcgaaaatat ccacattttt taaatttagt tcctctgctc
    22381 cccaatctct agttatgtct aatatttgca ttaatccttt tgcttccttt tttgaaacag
    22441 cagaactatt aaacttactt tcggctttaa taacactgta aactatattt tcatctaaat
    22501 taaattcttt cgaatacttt tctacatact ctgaatattt tttaggatat aaaaatttat
    22561 gtattacttt gctttccatt aatagtgccc caaataagat tataaaaata gatagaatca
    22621 atactttctt actattcacg ccaattctcc ttcatgtact caataaattt atgtgcttta
    22681 ctttctaatt cagttattgt tcccgaatta tctattatat aatcaccata tttggatttt
    22741 tcttcctgac tcatctgtga atttatccta ctaagtgctt cctgctcact gcaattatct
    22801 ctcttttgta ttctactaat ttgtacttct tgtttacaag taactactaa tagcttgtca
    22861 accatatcta aaaaaccact ttctaccaat attgccgcat ctaacactac tatattttcg
    22921 cctttttttc tcaatttttc tatttcactt attatttttt ctcttattcg tggatgagtc
    22981 aagttattta attccttaag tttttcttca tcactaaaaa ctattttccc aagagctttt
    23041 ctatttagag ttccatcatc attttttata ctttgcccaa aatggacaaa tactttttcc
    23101 aataacaact tatcctcaaa tatcttcctg gatataatat cagcatccac tataggaatg
    23161 ttaagatttc taaatatatt tgataggctg ctttttccac agcctattcc tcctgtaagt
    23221 cctaatatca acataaaatc acctactttg tctcatacca agaactacct acatttaaat
    23281 ctacatctaa gtgaacatcc atatttacag cattttccat ttcttctttt actattttct
    23341 taactatatc tatttcatca tcaattgctt caactatcaa ctcgtcgtga acttgtagta
    23401 taagttttga tttcaaattg ttctcttcca attttttatg aacatttatc atagcaattt
    23461 ttattatatc tgctgcactt ccttgtattg gtgcattcat tgcaaatctc tttcctctat
    23521 ttctttccat aaaattgctt gactttattt ctggtatata tcttcttctg tttaaaattg
    23581 ttacagcata tccatcctca ctagcctttt ctactgttgt gtccataaac tcttttattt
    23641 tatggtattt agcaaaataa ctttcgatat attctttcgc ctttggaaca ggtatattta
    23701 aatcatcagc tagaccaaag tcacttttac catatataat accaaagttt acagctttag
    23761 ctgcacctct caattcacta gtaacatctt ccataggaac attaaaaact tgtgaagctg
    23821 ttttggtatg aatatcttca tgatgtttaa atgcttctat catattttca tcctgactca
    23881 tatgtgcaag aactcttagc tctacttgtg agtaatcggc atcaaccaac ttacagttat
    23941 catctgcaat aaacactttt cttatttttc tacccatttc catttttata ggtatatttt
    24001 gaagatttgg ttctgtagat gaaattcttc ctgtagtcgt tatagtttga ttaaatgatg
    24061 aatgtattct attactttct ggatttatta tatttaaaag tccttcaacg taagtagaat
    24121 ttaactttac tatttgtcta tactcagtta tcttatctat tattggatgc ttatctctta
    24181 atttttctaa aacatctgca ttagttgaat atccagtttt agttttttta attattggaa
    24241 gctctaattt ttcaaataat ataacaccca attgttttgg tgaatttata ttgaatggct
    24301 ctcctgataa ttcataaatt tccttttcta atgtagctat tatttcttta aattcaacac
    24361 tcaattcatc caatatactt ttgtctactt ttataccttc atactccata tgacctaaga
    24421 cttctactaa aggcatctct acatgataaa aaagaccatc catactcata tccattagac
    24481 ttctttccat cataggaact gtttctttaa ctgtgcatat tatttgaccc atatactctg
    24541 ctaaatcctc aaattctaaa tcctcatagt ttttagattt aacacccttt cctaataatt
    24601 cttcttttga tttaaccttt tttgataaat acttcatagc tattgcacta caatcatatg
    24661 aggtagatga agttgaatct attaagtact cagctattgt tatatcaaaa tacatatttt
    24721 ctaagtttat attatatggt tttaaagata tataatcatc ttttaaatta tatccatact
    24781 ttttaatttc attatttgaa aatatactat ctaactttga cacttcattt gaatctacat
    24841 aatatatttt ttctccatct acacttataa aaatatatat tatatttttt tcaagtatat
    24901 taccttgtcc tctaacagtt tttaaaataa cttgtccttt tttgtttact tcatctataa
    24961 atttttctat atcttttaat tttaatattt ctattttttc ttctacgtgt tctgtgtcat
    25021 ctgaatcgac caaatcaact aatcttgata agagacttgt aaatccaaag tatctaaatt
    25081 tatctaaaag ttcatcttta tcataatcgc caaaaatcat actatctaaa tccacttcta
    25141 ttggcacatc tcttataatt gttgcaagtc ttttactttg aatagctatt tctttgtttt
    25201 cctctatttt cttttttaca ctacctttaa gcttatctgt attctctatt agattctcta
    25261 tactagaaaa ctcttttata agctttatac ctgttttttc acctattcca ggtactccag
    25321 gtatattatc agacttgtct cccataagac cctttaaatc gataaattgt gttggagtca
    25381 tctcatattt ttcaattaca gaattaaagt tgtattcttc tacttctcca accccttttt
    25441 tcgttataag ggtagttgta ttatctgaag caagttgaat agcatcttta tctccagtta
    25501 ctatgtatac cttatatcca ttttcttctg cttttttaga aacagtacct ataatatcat
    25561 ctgcctcata accatctatt tctagtctat ttattctaaa tttatctaaa aggtctttta
    25621 atggctgtaa ttgttctgcc aattcatctg gcataccctt tcttccagcc ttataatctt
    25681 tatattctaa atgcctaaat gtaggtgctt ttctgtcaaa agctacactt atatgtgttg
    25741 gtttatatat atctatcatt ttaaatagca ttgtagtaaa cccatatata gcattggtct
    25801 ttaacccttc tttattattc atttctggaa gagcataaaa agctctattt attattgaat
    25861 ttccatctat tataattaat tttttttcca aattatatca ctcctaaaaa ttatctattt
    25921 ttgtaacata tatattatat accaatatag tgttatttaa tattcccata ataataatag
    25981 tatattaaaa ttaaatatgt tgcaaacaat atattttttt gctataataa tatacatgac
    26041 atgcccgagt ggtggaattg gtagacgcag tggactcaaa atccaccgga ctaatactcc
    26101 gtgccggttc gagtccggcc tcgggcacca ttaaaaactc ctaaagctat ttattcaata
    26161 gattaggagt ttttttattt ttgatttaaa ttttaagtta ttttttgaaa ttactgtgtc
    26221 tttttaaaat ttatataatt atataaattt atcttctagc attaatttaa aaactaattt
    26281 acttttatat aattttctat tttatatcat aacttattac aaccttttat ctaatttatt
    26341 gtatacaaca acactacaaa taaattgaaa taaaataagc aatccaagaa caactaaaac
    26401 ccacttgctt attaccatta cagaaaatac actcaatata gaaacaataa atataagaat
    26461 tacaagaaca tagcacatga tttgattagt aatacgtcca gctttatcac gtaacataat
    26521 tcttctttca tcattcatct ctatcctttg attttttatc ttttcttcat aatctactgc
    26581 tctttcaggt ttactccaat acatatattg aagaatcata catacacctg gacctaaagc
    26641 agctccagat aatccccaaa taaaagcctc aaatgaaaac tcagtaaatg tagccaataa
    26701 aacaagtaaa attccacaaa taaaatatat aattccaaca tataacatat tctttttcat
    26761 ttttactcct cctcataaat aaatatttct tcaatactca tatcaaagta tctagctatc
    26821 ttaaaggcta agatgataga aggattatac ctaccatttt ctaatgaacc tatagtttgt
    26881 cttgatactt gcaaaataga agcaagttct tcttgtttta tgcctctttc ttttcttatt
    26941 tcttctaatc tatttttcac atctatcccc ctttaatatc tcaactacgg aaagtctact
    27001 ttccataatt atacaactat attccattta tgtaaagctt actttccata tttacaaaaa
    27061 aataacagac tattttcagc ctgtaatttt agaatttcat gttgtttatt aattttaatg
    27121 ttatttatta attccatatt atttattaat tttaacttgg tttctgaatt ggaatccaaa
    27181 cttctgtagt ataatcagga gcattgatat cgccattggg ataaagttca atatctggta
    27241 aattagcata cacataccca gaacttggta accactcagt aataattctt ttttgcaatt
    27301 cctgtattgc cagagcattt ggcattggag aaacacattc aaatactgcc catgtgcctg
    27361 atggtattaa gtattcatga gtatcttcta atacaggctt atctgttgga gcagcaatat
    27421 aataatctaa acccttacta tttgtacaca cactaactcc aagcaatcca caaggttcat
    27481 tatttattaa ctctgaaagt ttatcaattg ttccattttt tatgcattta gcccacaact
    27541 taggaacttt ctcaaagcac tcttctacaa tcatgttcat actttctttt ataccaacaa
    27601 tacgaaaccc tttcttttct acaatacgat attgcatttc tatatttcct tttacataaa
    27661 tgcttaatgt catcttagga tatgctttta aaacaacacc ctttgctcta gctgcagatg
    27721 gagaaatgtt atgtatattt tgaaaagctc tattaaaaga tgttggtgaa tcgtaaccat
    27781 acttctcaga tagttctaaa attttgatgt tactgttctg aagttcaaat gcagccttag
    27841 tcatacgtct tcgtctaata tattcagata atggtacttc tgcaatatat gaaaacattc
    27901 tttgaaaatg aaaggttgaa caacatgcaa tttttgctac ttctgcataa tccactccac
    27961 tttcgagatt ttcttcaata taagtaatag cttgatttaa tttatttatc cattccattt
    28021 gatattacct ccaagcaaat tatacagttt aataaaataa tatacctctc tattcatgca
    28081 caaaattgta agcatttaaa tttataagca tttttcaatc aatacaataa atataaaaat
    28141 tgtattatac aataaaaaag ttcaataaca ttatattgta aaatgttatt gaacttttta
    28201 taaaaattaa tttaagtgta gttaaattaa gtattacact atcttatata ttacaatatc
    28261 ctactatctt atagtcttct ggcactttat aatctttgtc tggagtacct aaattccatt
    28321 ttaaatcaaa catagttgca cttattattg ttgcaaaata aagcataact ataccaatgt
    28381 ctattttttc ttcatataaa ttagtatgac catcttttct aacagctaaa actacagtgc
    28441 ctccatcaac tataaatctc catggttgtc tatttaatgt agatggagct aatcttgcat
    28501 agtgaaatgc atcaagaagt gctctttctt ctaagatttc tatacttgca acatttcccc
    28561 attcgttcat ataaacaaca tcttctacac ctagtctagt agacccctta tctgttacaa
    28621 cttgcatatc agattttgaa taattttgcc cagtttttgt agcatttaat actttagcat
    28681 tacttacatt ttctccatat ccaatagcta taattcctgt tacctcttta tctgaattaa
    28741 ttccaagttt ttcttttacc ttttttccat cttcaaaagt aacccaacaa gaaccaattc
    28801 caagttctgt tactttaaag attatacttt ctcctatgta ccctgaattt tcaatgtaat
    28861 tatcttttat gtcagataat attataatat aacttggaga atctatcata tttcctttgt
    28921 accctgctac tttatctaaa ttattaaaaa cttcacttct attcataatt cttacatcaa
    28981 catctatttc tggaactagt ttcttacaag attctgagta tttcttaatt tcactaagat
    29041 attcttcttt aatatctgtg ttcttatatt ctctaacaga ctttctattt aatatgattg
    29101 ttttataatc cataatttta tcctccccaa actagtattg tcttaattat atacccatta
    29161 atatattatt ttactcattt atttaataaa taaagattac aattttgtaa ttattattta
    29221 ttaataatat tccactttta cattaataat gaccagttgt gcaaaatcca ttgccaacca
    29281 ccaaataaaa acatattata aaggtaatat aaaataacaa acaaatctta acaaaaaatt
    29341 aaactttttt tacacgaaag aaggcttccc gttgccttct ttatttttat aaaaaaataa
    29401 gctgtttcta aaatgatttc acatttttga aacagccaag ttattctata atgacaacta
    29461 taatttgagg atgtgaggga gagttgtcat tataaattta gggtaattat attatgtttt
    29521 aagctagttc tttctatatt tatataatac attttatata tgaattttat atgagattgg
    29581 tataaatgat atatgaaatt acatatgttt atattatatt aaattttatt attacatttt
    29641 ccacaacaat actcatatgt ctccacatta tttggaatat ttacaaatac aaattcttta
    29701 cagttctcac aatactttaa tatagatata gttccacaag tgtaacattc aaatattaca
    29761 caatcctcat tttcctctac tatttcaaac tttttactat gttcaccaca ctctgggcac
    29821 ttgtgattat ttacattcat aaatactcct ccttattagt atagttacat gtcaaaaact
    29881 taattaatgt attctatatt acttattata tattctataa taaatcatat tctaatataa
    29941 taaaacaaat taaataaaaa aaaaaccata aattttaaat ttatggatta attcttgtaa
    30001 tgataactct atttgaggat gtgagggagg gttatcatta caatagggta ttatattatg
    30061 gtttttaagc tagtctttct acacctcata tgatacatat gctatatgaa ttttatatga
    30121 tttttacata aatattttat gaaatttaaa ttttttagtc attttgaaat ttgtaaaaag
    30181 ttctctaaaa atccacagca tcataaacag ccaaaattta agtaaaacat cataaaaaaa
    30241 tttgtccaat aattttaaca agtaatctca cctcattata attattgaca caaacttaaa
    30301 taaacaatct ataaatcttc tataaatctt aaactttccc cctattgata cacaatttct
    30361 agtttgtcaa ttttattttg catttctaag aaaatttctt caacagaatt gttaaatata
    30421 ttgcctaaat agattctttt atctttttga aatgaataag attttacact accatcagaa
    30481 gaaatatata aatgactcca ctctgctcca tattttgaaa acctctcctt tgtatcttta
    30541 aacctcatat cttctatttt tattgctttt tctctgtctc ctcttactac agtgtgaccc
    30601 ctttttatta aactggttat ttttatatct tcttttaatt taatacgtgt atcatcattt
    30661 tttgaatata ctttccatat cactttatga cgataattat ctaattcctt tattaagctt
    30721 ctatcaatag taaaactatc tttagttacc acaattaaat caaaatggtc taacatatat
    30781 tgaaatattt ctctcatact tttatttttt aaaatatcct caccaataac ctctataaat
    30841 ctacagcctt tctcatacat gcagttcaat aaacttgtga tttcagaaaa ataatcattt
    30901 gtattattat ctccatctat ttctatagta aagtacaaag gagtaatgta tgatttattt
    30961 cctgttatct caaatacact tgatagtgtc tgtatatcat cttcaaacgt taagtacaca
    31021 ttctcataaa acataaaaaa ttccataact ttttctttca tattatggat atttctatat
    31081 ctattggata atatatttat tatatcgtca gtagtatttt tcccatcaca ttcagccagt
    31141 ataacaattt gcttccaact taagctatcc acatttccga tacttttttt atatccatct
    31201 tcttcgtcat aaataggacc aattataatg ttttcttttg gccacagatg aaccctagtt
    31261 ccatctttta atttcggata tttatttttt acagactcat aaaatctact tgtatcaccc
    31321 atgattcttt actacccccc atagtttaaa tattctatgc taaaaatagc actttatttt
    31381 tattaattca acaattattc aaaattggtc attatatatg tttaaatgga tttaacatca
    31441 accttattta tatgttattg atgtttatat atgttatata aaatcttatt tctcatactt
    31501 aaatgtaatc ttaactatct ctggtttaca attaagccta attaaaactt ttgagcctcc
    31561 taagccttta tttaaaatca tagtgctatt atctttttca tattttcccc agtcatactt
    31621 aggaaagaac tctctatttg gagataatac acctccaaca aaaggtattc ttattatacc
    31681 tccatgaaca tgtcctgcta aaaccaaatc tgctccccat tttgcatatt tttcaaagaa
    31741 aaatggtgta tgtgctaaaa gaatgttata ttcattttca ttaactttac ctagattgtc
    31801 ttctacaaaa ttgtcactta gttctaattt tttgttatta gcaaataaat atggatagca
    31861 ttccaatggt ataattaaac catataaatt aagatggcta ttacctctat atattcttac
    31921 agattcattt tccatattta ttattttctt tgaatatagt tccttaaaat aatccttata
    31981 tagatgttta tactttttta ttaaagattt ttgttcatga tttccaatta tatgatatac
    32041 ttcatatttt tcaaccaaat tatctattaa atctaatgca acttgaaaat ttttattttc
    32101 tccatctact aaatctcctg ttataaatat aacatctggg tttaatgatt caattttatc
    32161 tatcaatacc ttattatttt tatcaaacac cttattatgt aaatcactca cttgaactat
    32221 tttaaagtta tcaaattcct ttggaatttt tttgttgaaa atagtatatt ctgtcgtttc
    32281 tatatgctga gtttcatact tatacagaat atacaataat attaatacta atactgtcat
    32341 tgaaaccttt ataatcatat tctcttcctc tttattctat tttgttaata ttattataat
    32401 gcttttatgt tttttttaaa acaataacat catcacatac atataaatct tttcgacatt
    32461 ataaaaataa gttatccttt tctaaaattt tcatttattt tagaaaagga taactataaa
    32521 attatctata aacctctata ttgtgaaata tagtttttcc tttatttaaa tttttatata
    32581 tatgctctaa agttccatca aacctaatag aatgtccaga ctctaaaata taagttttcc
    32641 catccacatt aaggtcaagt ttaccttcta ttacgagcac atattcttct aaaacatgac
    32701 tatgtgtaga tgaaacatga gtacagtttt cctcaagctc aattataaaa agttcaaacc
    32761 ttctttgagg gctaaaaggg tatattggat ataacttcat gttccctttt tgctcaacaa
    32821 taggctctac ttcttctttc tttataacct cataggtatt tgtattttca tttaatagtg
    32881 tagtaaaatt tattttaaat ccagatgaaa ttttccataa agtagatact gtcggacttg
    32941 attcacctct ctcaatctgt ccaagcattg ctttactgac acctgtcatc tttgaagctt
    33001 tgtctaaact tatgcccatt tcttgtctca ataaccttaa attttctccc acattaggaa
    33061 atttattatc catgttaaaa tcctcctgat agctatttgt attctatgat ttttactcct
    33121 gtaagttcta ctatctcttt taattttaac atatgattgt tacaaatatc atcaaattca
    33181 tatatataat cttcaagccc taatgcctta tatttatcga ttccaaattt atgatatgga
    33241 agtaactcca tcttgcctcc tggaacattt tgtttgacaa ataatgcagt atttcttata
    33301 ttttcttcac tatcatttac tccataaata attggcactc ttataaccat agatttattc
    33361 aactttgaaa gcctacatat attatctaat ataattttat tacttactcc tgtatattct
    33421 ttatgaatat tatcatccat agattttata tctacaaata tgtggtctat tttctcaaat
    33481 acatcattta ccttattcca atcaaaatat ccacaagttt caattgcaat atttatgcct
    33541 ttatcataaa aaatatcaac taattctctt aaaaaatcaa tctgaagtgt aggttcacct
    33601 ccagaaaaag ttatccctcc attagattca aagaaaaata taaaatcttt ttcaacttcc
    33661 tctactattt cttcaacact catttcttcg gtcattatac aaatggcatc tttaagacat
    33721 accttgatac actctccaca taaatcacat ttattattta tttgcgctct atcaaatagt
    33781 gaacttatat tttgaggaca aacatctata catctattac aggatacaca tttatctttc
    33841 ataacaccta attttactat gttactccag gaatctggat tagaacacca cttacatttt
    33901 aatttacatc cttctaaaaa tatagtagtc cttattccat ctccatcatt tactgaaaaa
    33961 tgttgtactt ttacaactct acctttttta ctcataatct acctcataat taaaaattgc
    34021 tatgtgaatt tctagctatt atagcatctt gcatttctct tgataaatta acaaattgag
    34081 tactatatcc agcaaccctc actaataaat ctttatattc ttctggattt tcttgagctt
    34141 ttaatagagt atcatttgat atcgtattaa attgtacatg gaatcctcct aaagcaaaat
    34201 atgatttaat catagatgtt agatttctaa gtcctctttc agtttccacc aaatcttgat
    34261 tcagtcttaa atttagaagt gttccaccag tatgaacatc atgatttatt tttgatgcag
    34321 atttcattgc tgctagtgga cttgttgtat cagtaccaac aaatggagaa actccctctg
    34381 tcaaaggttt tgtcgctttt cttccacaag gagttgcacc tactattttt ccagttggta
    34441 catagtttga tatacccata aatgcactat taaatttaga gccaaaaata tccttgtatt
    34501 ttctagtttc agtataataa aaatcagaaa cttcaattgc taatgaatct acataatcat
    34561 tatcatttcc atacttagga acatccaacg caagttttct taattcttca taaccttccc
    34621 aatcattatt tagtgctttt gttaattctt ctaacgtagt caccttatcc tcaaatacta
    34681 atttttttat agctgccaat gaatttgaaa ctacacctaa ccctatacca gttaatacag
    34741 gacctatatt atattttgct cctcctttac ttaaatcaac tcccttatcc aaacatccat
    34801 ctacacaagt agataaaaat ggtcttggta ccatttcttt atgaatttgt tgagcaacag
    34861 tagttcctat aacagaactt tttacaagat atgctaattg ctttaaaaac tcatccttaa
    34921 gttcttcaaa agaagtaaat tctgtgatat tttttgtatc taaccccatt ttttctccag
    34981 ttaatctact cttaccttca ttcatagcat attctaaagc tgctgcaaaa ttgatattta
    35041 cggctgaagt ccactgtcct gtttttctaa aatgaggaac tacacaacca cagttactcc
    35101 aatctctagc atcttctgca tcatatccat cttgtaataa catttgtgcc cctgcactat
    35161 cgctgtgtat agcagggaaa ccagttcctt gttttaccaa tttagcaact gccattacga
    35221 aattatctgg tgctccttga ctcactctaa cacttaatcc tggttggtga gtttttacac
    35281 tttctgtagc ctttaaacac atatatgaga tatcattcgt tccatcagta ccatttctct
    35341 ttttaccacc aacagtaaga ttttggaatt gattatatcc tgcaaagtaa tttgctgtat
    35401 ttgctgatat agtccatacc cattctgata acttaatcca aagtgcttct atcaattctt
    35461 gagcttctga ttcagttatt ttaccttctc tagcatcatt ttcataatat ggatacatat
    35521 attggtcaaa tctacctaaa tttaatgcca atggattttc tgatagaata ccacctaatt
    35581 gaacaaacca tacaaattgt attgcttcat aaaaattagt tggagggttt gctggtacat
    35641 tcatacatat ctctgaaatt tttataagtt ctaactttct cttttcatct gtttcttgct
    35701 tactcatctc ttttgcaaga tttgaatatc tttgtgccaa tgtcataata ccctgtgcaa
    35761 ctatagaaac agatttgtaa aaatctatct tttcaatatt ttctgaaact gatatatcta
    35821 attctttaat ttttttatct gcatcttcct taatcccttt atatcctttt acaaacaata
    35881 tatcttgata atctggtgtt acttcaccaa ctgcctgtct ccacttagaa tcattatcta
    35941 ttattcctgt atctactaat atcttagctg tatcttctgg gattcttgct aagaaaactt
    36001 cttccaaaga ttttcccttc caatatggaa atatttcttt tcttataaat tctatttgtt
    36061 cttttgatat tacatatggg tcttgagttc ttttatcaaa agtatccatt tctttgtcta
    36121 cccattgcca agaaaactct ggtgtcaata tccctgttct tctaaatttt cctgctgtac
    36181 ctactattaa ttcattttca aagattttta taggaagttc tttacatgtc tccaaaaaag
    36241 ctttagctct tcttatacag atagcttctc cctcagtttt tttatgtgat tctgtaaaaa
    36301 ttctagccct ttcacaacat atcataggtt tagaattaat atattcttgt ctaaatgctt
    36361 ctattctatt agtttgactc atttaaaatc cccctttgga taatatatac tacatttttg
    36421 tatgttataa catactttaa ttatattata ctgtccttta gcataaaatg gaagcaaaat
    36481 aacttatttt ttttcatcta ccttgaacac ctatatatta atactttttt tgttaaattt
    36541 ataaactcta aaaaaatttt ctatgatata ttttctatat tgttatagtt atttctaata
    36601 tactaatgat ttgaaatatc aaatttgtat actagactta gttttaaaac cttagttgat
    36661 tgtttaactt tgttaacttc aacatgtata tccttcatat aaggaagctc tacaacacct
    36721 actcccttat taaagtcttc ttcaaccata gaaaaaattt ctttctcagt taagttcgta
    36781 ttagtcaatg acttaaataa attgtatata aatttaatat atttatcact ctcagataac
    36841 ttatcatctt ctggatactc tttagcacag agaatatcta ttgttacttt tccttcttta
    36901 tcgtcttcag acatagaata gaataaactt cgaatatttt tttcaggtgg tttttcagca
    36961 ccatttatag atactacaaa gctaccatct gattttgagt cagtaatact ataaccactg
    37021 tctattatag ctttcgaaaa tctttgagat aaatcttttt ttacttcttc tctacctttc
    37081 gcatcattaa tcttttgtga gtaattatta aaaagctcga tagattcatc ttttttatct
    37141 tcaacttgtt ctttcaacga atctttacta atttgttctg agctacttaa gttcttatca
    37201 tatacaggag acatacaccc taccatcaat gctaagctaa gatttaaagc cacaatgatt
    37261 gccactctat ttatattcct ttttactttc atacacaatc ctcccatcac actaaccgct
    37321 ctaaagtatt acttattgca ctagaatatg tttatatttt ctttatatta aagatttatt
    37381 attcaataca tatcaccttc ctatccaatt cttataactt actattagta aaattataag
    37441 ctccttaata cagaaataag gatgttatct tttgttatta aaagataaca tccttatttt
    37501 tttgtatcaa tacatatgtc ccaactggta acttaatcgc cccaactgtc agtatcttgg
    37561 tccaatggta tatatatatt tagtataaat ttatcgatac tattttcaaa taataattct
    37621 ccattatatt tttgtaaaga agatttaata ctctgaatac ctattccatg tacaaactta
    37681 tccattttgt ttgttaacaa tatattttta ttaaaagtaa gctgatttag tttactattt
    37741 tcacatttaa gtacaaaaaa tgaccttgtc atagttcctt taattctaat atatttatta
    37801 acattttcat ccactatttt atcacatgct tctattgcat tatccaaaat attagaaaat
    37861 atgcttgaca catctatagg ttttacaaaa tttacttttg aaaaatttat agagcaagta
    37921 aatttaattc ctttttttaa acatatttca ctcttttcgt tgattataat gtctaatatc
    37981 atatttcctg tattatagat atatttaaat gtttttattt catcttttaa attgttaaca
    38041 tattcattaa tttctttgct attgtttcta aggttgtcta tacaataaat gtgattattt
    38101 atatcgtgat atagtttctt aactcgcata tgagattctt gaatacttaa ataatgcgca
    38161 tattgcatat ctaacttatt ttttattaat tcagcttgag attttaatct attctctttt
    38221 attatttctt taaacaatag tataagaaat atactactag ctcctaccaa ttttggatta
    38281 acaaaattat caaaatagta tccttctgaa tgtaatgacc caaaccgttc taattttttt
    38341 aatacataat ttacaagcat attcattgta ttaactaata aggcaaataa taaatataca
    38401 taatttctct tatcttggct aattaatttt atagacttcc taaaaaagct aaatataaga
    38461 tatataagaa taattattaa attactatat atctgaattt ttatagattc taagtgaggt
    38521 actaataagg attctttaga tgaaataaca ttacctgtaa ttgcaaaaaa tagtattcta
    38581 tatataacag catccaaaca tttataatat acactaaata gataaaagaa aatggaatac
    38641 ttaatataac ccatcttata aaaataatta tagtatcctc cccaagcaat aaatgctaat
    38701 atataaaata aatagtttat attactaaag taatttcctc cattaaataa tctacttatc
    38761 tgaattataa tataaataag aaacaagaca atatagaata ctattttttc atttttttta
    38821 ctatcctttt tataaattaa aattccgttc attatagaaa actgtataaa tatagataaa
    38881 actactaata gctcagtcat aaagtttagc atattgtatc accaagaaat tttaagaatt
    38941 tctccttaac ttctttatat ctatatctac taataggtac ctcttctcca ctttccagta
    39001 ttgcagtatt aggctttata ttttcaacat atcttaaatt tactataaaa cttttatggc
    39061 atcttataaa tttatatgga tttaattctt tttctatttt gcctaaacta tattttatat
    39121 caaaattctt gtttattgtg tgtattagca tatctttttt ttgaacttct atatatttta
    39181 tttcatttaa ataaatctta tatgtattag acttattttt tattgtaata tgactttcct
    39241 tatttatttc aatatctttg atacaagtta gcacatgttt ttttagttct tccaactcta
    39301 ttggctttag taaataccta taagctctta cctcataacc ttcttgcaca taatctatta
    39361 atgaagttac aaatattatt tccatttcat tgtctacttc tctaatcttt cttgcagtat
    39421 ccattccatt taatttttcc atttttatat caagaagtaa tatatctata tcttttaaat
    39481 tactttccag taactcttct ccagatatat acttttgtat ctcatattct atacttatat
    39541 cttttaatat attctcaagg taatcttcta ttaaaagttg tgtttctttt tcatcttcac
    39601 aaactgctat cttaatcata aaatgcctcc ttacataatt tctagttaat acaattaaaa
    39661 tatttataat atatttaaag ataattttgt atatctgata ctttaggata acattttcaa
    39721 attattttta tcaatactca atcccatata actattattt gatatttcaa tttaaagaca
    39781 gaaattacta taattaaaaa cagtacttac tgataaaaga agatgtctta aactattaaa
    39841 aaaataattt taggacacct tcttttattt atttaaactt tttaagcctt aaataataca
    39901 ttaatctcct atataattgt actgcatata aaatcaacaa tataatctgt agtacaatag
    39961 cagttacaga tgctccataa ccactatata aatttcttat ttcaatattc aataaatata
    40021 taaatattac tactaacata aagaatataa acatattcat aaataaaatt gactttactt
    40081 tgtttttttt atcagaatca ctatataatt taggaggtat gtctttgtat tctttcgacc
    40141 atatatacca tgcagatgta cccataaaac taccaatact cctaaataca agattccagc
    40201 catttgattt atgttcatca aaataattat tctctacatc atagtagtaa tcaacagtgt
    40261 attttatttt tcttggactt cccttatgaa aataaaatat tcctccaaat ttatttatct
    40321 tatataaatt atatccttta gcttcaatat cttctaacca tttctctatc ttatcaggtg
    40381 aactatacca gtcaaatttg aattttattt tcttttccat agcactcatt ctccaaaatg
    40441 atatttttaa atagcttaaa aatctattta aataattata tcattttgat actaatttta
    40501 ggttacaatc atatatttaa aaagctattt aaatagtaat aaactaatag ccataacacc
    40561 cattcctgca atcaatccat aaatagataa atgatgttcc ccgtactctc tagctgatgg
    40621 caataattca tctagtgata tgaataccat aattcctcct acaattgcaa atacaattcc
    40681 aagagttata tcatttaaaa aatttctaag aagtacatat ccaataatcg ctcctaaagg
    40741 ctcagacata ccagataaaa aggaataata aaaagctttc tttttatctc ctgtggcata
    40801 ataaactggt acagatacag atataccttc tggtatattg tgtattgcaa ttgcaattgc
    40861 tatcggaata gctattgtaa catcatcaag agctgatata aatgtagcta atccttcagg
    40921 aaaattgtga attgctatag ccattgctgt aaatatacct gttctcaaaa gagacttatt
    40981 ttttttgata ttcttttctt ctatattctc cattttcttt atttcatgtg gattttcttc
    41041 ttgtggaatt agcttatcaa taacagcaat gatagccata cctataaaaa aagctattac
    41101 agttgaccaa cttcctaatt tctctcccat agattttgtc aaagcatctt gtgctttagg
    41161 aaaaatttct atcatagaaa cataaatcat aacaccagca gaaaatccta agcttataga
    41221 aagaaactta gtatttgttt ttttagcaaa aaatgctata caacttccta taccagtaga
    41281 taaaccagcc aataatgtta tcaaaaatgc aaatattaca ttattcaaat aaatcacctt
    41341 ctttttaatt gatattgatt atcattttct ttatgctatc atatatattt tatatagtca
    41401 atatttataa aaaatataca caataaaaaa gcagagatat aaacttagtc tctgcttttt
    41461 ctttatagta tattaaaatt atttatttaa gaaatccact gcatattgca catataaagc
    41521 agttccaatt tctagagcat cttcatctat attaaagcat ccatgatgat gaggataaca
    41581 agcacctttt gactcatttc tagctcctac aaatgctagt gcaccaggtg ctatattcat
    41641 aaactcagct aaatcttctg ctcctgttac tttttcaaat agtgttatac agtcttctcc
    41701 aaatagctta attgctgcat ctgtagctat ctttgaacat tctttgtcat ttataactgc
    41761 tggtgttaag tacccatact ctaattcagc atctgctcta tatgcttctg ctgtagattt
    41821 tgctatacgt tctaaaattc caggaatttg ctttctaagc tctggattaa atagccttat
    41881 tgtaccttct aagacagctt cactcgcaat tacgttaaat ctagttccag aatttaaaac
    41941 tccaacactc acaactaatg gttccagagg acttacttct ctactaacca tagattgtag
    42001 attcataact attgctgaac ttgcaagcac tgcatcaaca ccttgatgag gaagtgaacc
    42061 atgaccacct ctacctttta cagtaatttt aaagaaatca gcagaagcca ttcttggtcc
    42121 ttcttctacc gatatggtcc cagattctac atcagtccaa agatgtattc caaacacact
    42181 atctacacct tccatagcac catcttgaat catagctctt gcacctttac caacttcttc
    42241 tcctggctga aaaaataatt ttacagttcc attgatacta tccttaatat catttaacac
    42301 ttttgctgcc cctagaagca ttgatgtgtg accatcatga ccacaagcat gcataagtcc
    42361 ttcattttta gatttgtatt ctacatcagt gcattcaaca acttgtagag catccatatc
    42421 acccctaagt gctacagtct ttcctggatt agctcctttt atagttgcaa ttacacctgt
    42481 acctccagct gatacatatg gtatccctat tttatcaagt tcttctttaa ctctctttga
    42541 tgttctcact tcttccatac ttttctcagg attttcatga aattcccttc taagcttaat
    42601 tacatagtct ttataactct ttgttatttc tttaatatcc attccatacc tccaagtagt
    42661 acacattctt cttatttaaa atacttttta tcacttaaat actatattag atttacaaag
    42721 atacctgcta ttactacaga agctattgta actgtagtaa atcctccaac aagcatcttt
    42781 ggaagcatct tatccattaa aaattctttt tcttctggtg tttcagccaa agcctttact
    42841 gcctcttcag ttaatatata gtttggtgga aaaccataaa gtgatgtaag cgaaactgaa
    42901 aaagccattt ctttactttc acctaaaagc ttacctacta taatagagaa tattcccata
    42961 cctattacac caaatattat gattcctaca agaggaactg ccatttcaac aagcattgct
    43021 ggtgtagctt ttgctaaccc tgcaaatata aaagccatca atgatgtcat taaccatcca
    43081 aatgaaccag atttattaag tggttttcta tcaactattc caagttctga tgctattaca
    43141 ccaaatatta aacatataac atatcttgat acaacttcat ttgtaagatt tgtaaattgt
    43201 actgctaacc atgctacaaa tcctaatcta agtattatca tatatgtcgt atcatatttt
    43261 tctggtaagt ctggtattat cttaaatctg cttttttgtt caggtaaatc cttaactttt
    43321 tcttcatctg tatttacagt tacttttcca cttctaaagt ctgataaaag tctttttcct
    43381 tcttttttaa gcattaatgc tgtaatagga taacccgcaa atccttgtgc aacatacatc
    43441 aaaattgcaa gtactgataa acttgcaaga ccttttgctg tagcagcatc tgccatcatt
    43501 atagatgcaa ctagccctcc agtaagagga ggtgtagcta ttactgctgt atcccatcca
    43561 aatactactg tccctaatgc caaagcacct acacatattc ctactattcc tgccaaggct
    43621 attactattg ttttccattg acctgctaat tccttaatac tcatcatagt tcccatatga
    43681 gttattaaca aaagcatacc taattgagct aatggtgttc ctaaagcggc taagtccaca
    43741 atattttctg ggaaaaaagt ccaaaatcct actataaata taattgctga tacaaaaacc
    43801 gacggaacaa aagctttagt ttttgctgaa actatatctc ctatcgtcag tgatactaaa
    43861 actagacaaa aagctgttat tgcctccata caaaatcccc cctcataata aatatatttt
    43921 tataaaatgt atgtatagtt atccattatt ttactacttt tgaaaaacat ttcaagtcaa
    43981 aattcgattt ttttcataaa attcgataaa ttccataaat taaagttagt tttctaaatt
    44041 attctttttg taaaaacgtt atcccgacca ttggctattt aactctattc ttacaaagag
    44101 aattaaatgt aatttgcaaa aaataaaaca taggatacat tttattcata aaacatctta
    44161 taaatataaa atgaattgtc tttatgaatc ttaatatatc ctatgtttgt aagtttaatg
    44221 tgtatgttag ctatggtgta aaatttgtat actagttatt gtgtaaccta ctatatactc
    44281 catttttgtt tactagctct tcatgagtac ctctttcttc aattccttta tccgtcaata
    44341 ctatgatttc atcagcattt tttatagtag tcaatctatg tgcaacaact aaatttgttc
    44401 tatctttact taattcctct aaagactctt gaatttctct ttctgttaca ttgtctaaag
    44461 cagatgtagc ctcatccaat attattattg gaggattctt taaaaagatt cttgaaattg
    44521 aaattctttg tttttgccca cctgataatt ttacgcctct ttctcctata taagtatcat
    44581 atccatctgg caaagtctct acaaagtcat gtattctagc ttttttagca gcagctatca
    44641 tttcttcatc tgtggcatta gggttcccac aaagaatatt atctcttata gttcctgtaa
    44701 ataaaaatac atcttgttga actataccta tattttttct caatgatttt aaactaacat
    44761 cttttatact ctttccatca atttttatat cacccttatc aaattcataa aatcttggca
    44821 ataaattaca taatgtagtc tttcctcctc cagaaggtcc aactaatgct atagtcttac
    44881 cagcttttat agataaactt aaattatcca aaacttgagt cttatcttca tatgtgaaag
    44941 atatattttc tatctcaatg tcacctttta cattttcaag ttcaatagga tttttagctt
    45001 ctttttgatg gtcttgttcc attatctcta tgaatctttt aaaacctgtc ataccatttt
    45061 gatattgctc tgtaaagttt attaattttt tcataggttg tataaacatt tttacatata
    45121 gtatataagc agcaaaatca cccaaagtaa tctttcctat ataagtaaaa taaccaccag
    45181 ctattacaac tacccactct aagatatcca tgtataaatt cataccagag taatattctg
    45241 ccattacctt ataagaatct tgtcttgctc ttctaaataa tttattgctt cttacaaact
    45301 tatttaactc ttctttctca gtacaaaaag acttagttac cttcatacct gctatactat
    45361 tttctaaagt agcatttaca tcacctatag taactctact cttcataaaa gcaatattca
    45421 tcttatcttt tttagctatt gcaaaccaaa ttataaacgg taatatagca aatattataa
    45481 gcgtaagagg aatatttata tctataagta taaagaatga accaaccaac attactatag
    45541 aaatgaataa atcttcaggc ccatgatgtg caagttctgt tatatccatt aaatcattta
    45601 ctattctaga catagttact ccagatttgt tattatcaaa atacgtattt ggaagtcttt
    45661 gtaagtgtgt aaatacctca tttctcatat cagcttgcat atctacacca ataacatgtc
    45721 cccaatactg cataaaatag ttaagccctg cttttgctat aaatataagt ataagtgcta
    45781 ctgcaaaaac tactaacatt cttaagtttt tatttggaac aacgtcatcc attatatttc
    45841 tagttatcat aggataaact aaatcacata aggaaaatag aaaagctgct ataaggtcta
    45901 gtgtaaaaat tttcttatat ggcttataat atttgacaaa ttgttttatc ataagtatta
    45961 aatccctcct ttattagtac ttctattagt acttctaaaa aatacccata aattagtata
    46021 acataacaat aactttaaat cttttataaa cttacaaagt aataacaatc tttttcataa
    46081 aattgaagat ttatatcata tagttgtata cttgaaacaa cataaaaaga gctatatctc
    46141 tatatttaaa attttataaa gatacaactc tatatttaat actaaatttt ttttataaat
    46201 acaaaactac ttcaatgcat ttaatatctt tttatctaat gctgttgtat ttatacctgt
    46261 tatatatcct gttgccttta ttgtaggtat atcataagtt gttggtagta tagtagtaga
    46321 tacgattaaa tcagcacctt cttgttttgt tgaacattca gcaattttac attgctcaac
    46381 ttctgcttgt atattatttt ctttaaccaa tctttctact ctgtcacaaa ctattgttga
    46441 tgttgcgatt cctgctccac aggctactaa tatttttttc atatattgtc ctcctacaat
    46501 ttctttaata ttttataact caaaataaaa ttgataaatt tcttatatct ttatttatat
    46561 aaataattat atcattttat ataagaagaa catcttaaaa ttattctttt aatatgtttt
    46621 ttatttcaac attgcttata cttaaaattt cattttttat acaagactct atgtactttg
    46681 atatattttt ataatcattt aaattaaact gttcaatatt caatttatat tctatatcac
    46741 taacaatacc tatcaaatca gacttattag ataaaatatc ctcatactta ccttttctag
    46801 ttacttccag tcttttaaat ctataatttt tatatccttc tatgataatc aagtccatat
    46861 cttcatataa ttgaatcatt ttatataatg gattcttctc ttttagttct tcaatatatg
    46921 ctactcgttt atctgatatt atacagactt ttctcgcccc agattttcta tgtttataag
    46981 tatcttttcc ttcctcatcc atatcaaaat catgacaggt atgtttaata actccaacac
    47041 gatacccttt tttatttaat tcttttatta tgccttcaat caacgtagtt ttacctactc
    47101 ctgaatatga gacaactgac aatatattat acataaaatt gtgtcctttc ataaaactat
    47161 ctaataacaa catccactaa ttgattttct tttattgtct cattcttttt aatcttaatc
    47221 atacaattac ttcttagagt tgcagatagt ctagcttttg catctttctt atcatttagc
    47281 tctacataca atttaccttc acttgtatat agttttccac ttacaaatct atctttattt
    47341 gaaaatttat taaattctcc tttaaatata gcttttatat gatgtgttcc cttcttatat
    47401 gttaaatact caataacagg aactatcaca ttatcaaata ccactattgc tgcatgtgga
    47461 ttaccagaaa gaccaaataa tagcttttct cccaatgtac ctacataaca agctccacca
    47521 ggtttaatgt ttatcttcca aaataattct gttgccccta tttttttgaa tacatctgga
    47581 attaaatctt tatctccaac agaaacacca cctgtggtaa ttaaaaaatc tacactctgt
    47641 aacatattct ttaccttttt ttctaactcc aatatatcat cagaacaatt gcatattgta
    47701 tctgcttgta ttccaagttt taataatctt gaatataagg tatatctatt actatcgtat
    47761 attttaccat tctctaaagt ctcatttata tcttgtagtt ctgtacctgt attcaaaatt
    47821 ccaactttta aatcatcata aacagttact ttggatattc caagacttgc taatactcca
    47881 ataccatcat atcctatatt ctgattttta ttaataatca attgcccctt ttttatatcc
    47941 tcacccttaa atatgtaatt atcgtattcc tttaatttta tatttaaagt tatcatatca
    48001 cctataactg ttacatcttc tttttttatt acacaatttg caccctgtgg tatctttcca
    48061 ccagtcatta tcttcactgc ttcattttta tttaataccc tattaaatac atctcctgca
    48121 tacaaagtcc ctataacctt taaatttcta ttttcagaat ttgcattata tgcataacca
    48181 tcatagggtg acctatcaaa tggaggacta tcaattgttg aatatatatc ttctgcaact
    48241 accttattta ctgcatctat aagatttact atcttgaccc tgtttattgg taaaacattt
    48301 ttttcaataa tttttatggc atcttctaat tctatcattt taaatactcc ccaatatatt
    48361 ttttatttaa gatatttcta tgtaatatat tatagattat aaaagttgga aatactttta
    48421 tcttatattt aattataact aatagttatc tataagtgca gttcaaattt tataaatcta
    48481 ataaaaaaac tgttcccagc tagaaatttc cttctatctt gaaacagcat catatgcttt
    48541 agaatatttt attctaataa agttcctttt attattttta caattttatc actttcaata
    48601 gtttttatag attttatttt gttatccaca atacttattg taagtacctc tttttttaat
    48661 attttgttat cttctttcaa tctataactc ttaactactt tactgccttc atgattatat
    48721 gctgttaaat cataattcgc atctcctaca atttcattta ttctggaatc ttcaacacca
    48781 acatactctc ccaggtcaat atgtctaaaa ttttctagtg atacagccga tttttcatgg
    48841 tcactttttt caatcactat actagattca gcatcaatga ctgatatacc ttgtgatgag
    48901 aaagaattgg tttcaacatt gacaacttta ttttcgttaa tatcaacata taaagcactt
    48961 ccatctagtt catcatttcc agtatatttt ggataaacta atgtcatata acttgatgta
    49021 tttatatctt gcattgtatt tgattctttt aaaactttag gttttatata ataaaatgta
    49081 ttataaggta tacccatatt ctttataatg tacttataat ctttattcat aatttcatta
    49141 acatcatttt gaacttttgt catagttttt tgctgtgttg ctttacttgc tacttgattt
    49201 ttctctttag tattttcttt atgacatcca acaagtataa tacctaaaat agttacacaa
    49261 actaatgacc tttttaactt cataaatcaa tcctcctttc ttaaatttta tagtatttat
    49321 ctatatctca atttacagtt atctcttttt tcaataatta tatttatt
//
LOCUS       DAMJVI010000017        48623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.017, whole genome shotgun sequence.
ACCESSION   DAMJVI010000017 DAMJVI010000000
VERSION     DAMJVI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 48623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.017"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            142..1137
                     /gene="ilvC"
                     /locus_tag="PC545_001075"
     CDS             142..1137
                     /gene="ilvC"
                     /locus_tag="PC545_001075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDF2455863.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVH
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLTMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            1190..2878
                     /gene="ilvB"
                     /locus_tag="PC545_001076"
     CDS             1190..2878
                     /gene="ilvB"
                     /locus_tag="PC545_001076"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HDF2455864.1"
                     /translation="MSGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFRVANSGRKGPVLVDVP
                     KDLFLAEMDFSGEDYDLCQIDDYMDYKSDFDLDDETNIKLLNEAIDIIKESKKPIIYA
                     GGGVKSSDSEEILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLVGDVKLVLS
                     LLIERVESKNNSNWKEEIKRFRKSEGVQTYEFHPQNILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGTKVGNVDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNQRYSQTDINENVDYVKLVNAY
                     NIDGYKVSSMEELGKALDMIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            3089..3766
                     /locus_tag="PC545_001077"
     CDS             3089..3766
                     /locus_tag="PC545_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2455865.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNSVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDINPPS
                     FEKPLKK"
     gene            3940..5976
                     /locus_tag="PC545_001078"
     CDS             3940..5976
                     /locus_tag="PC545_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HDF2455866.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNDEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITKKELYKEIAIKTIDYVVREMKDKEGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGIFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIADLSKKV
                     FEYRKERTSLHKDDKILTSWNALMIVALTKAYSTLKNDIYLEYSNKCLNFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCINLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNRLEEMSYKQ
                     LKLYVDNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLIAFKELISENFIPNAT
                     FLAKKYNEENTIIGFLNNYILKDDKTSYYVCQSNSCSQPINDLQKLKDMILGVE"
     gene            complement(6167..6733)
                     /locus_tag="PC545_001079"
     CDS             complement(6167..6733)
                     /locus_tag="PC545_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2455867.1"
                     /translation="MDYSNELKELFLMNQTYATLFTLTNKIQIEGDKYFGILTSRQYM
                     TILSILHLPEEETTLNNIARKMGTSKQNINRLVANLEKNGYVDVIPSPHDKRAINVKV
                     TDLGKKVMVTCSRTGINFMADVFHEFTKDELETLWSLLKKMYRFNGEEQDGFEEDANF
                     MEYEEIDKIKSEALEEFAKRRNRVNKKD"
     gene            7021..7956
                     /locus_tag="PC545_001080"
     CDS             7021..7956
                     /locus_tag="PC545_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2455868.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKKPKAVIQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGKQGIILNLAHLIINREI
                     KKYGRRSKSNKINNLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLVQGLKEIEDKENLKKVPLDIPIYIISGDKDPIGKNGKGVLRLRDRYIKLGVKDVT
                     CKLYKDGRHELLNEINREEVFEDIICWLNNKIEIL"
     gene            8421..9635
                     /locus_tag="PC545_001081"
     CDS             8421..9635
                     /locus_tag="PC545_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455869.1"
                     /translation="MQIKIGKVIQCLRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVMKIYKELESGFARIEIDLSTEEPKEEF
                     RQDLESVKKLSNMYIEKYPKSYLLKLRICSLYQMYSYKFGKSELNDRVKETMNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKSIGDTSVNLAMIYLKQNRL
                     EEAEILLQNKLFSNVFNISMDCKGIINVYKNQYKELKKKLENRNFNKNAIEREMEYIK
                     NKLLGYANLSLEIKKMLSEDKGAFFSMYMDYMELSLIFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVESKNLYTFNIYTNLLIVLNDDSYNELREEPIFKDVIEK
                     ILDMEKMLKNKE"
     gene            complement(10055..10576)
                     /locus_tag="PC545_001082"
     CDS             complement(10055..10576)
                     /locus_tag="PC545_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455870.1"
                     /translation="MKFKRLRTSENKLCYKAMELYKISFPFHEQRKSSLQEEILKNKE
                     YQFNLIYDKNQFVGIILCWETDDFIYVEHFCIFPEMRNNRYGQRALELLNKKGKTIIL
                     EIDLPIDEISIYRKAFYERVKYKTNNYEHIHPPYHEKFNGHNLIVMSYPEKLSKMEYD
                     KFNHYLRNNVMSF"
     gene            10932..11729
                     /locus_tag="PC545_001083"
     CDS             10932..11729
                     /locus_tag="PC545_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2455871.1"
                     /translation="MEKREKRIIISSLLSVSILMGLVSIYSILNKEDIILTVKGQEQK
                     VSSFKKTVEELLDEQGIKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGQEGEKKLVYNLTYHDGKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGGKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            11839..12429
                     /locus_tag="PC545_001084"
     CDS             11839..12429
                     /locus_tag="PC545_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDF2455872.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLARFNEDVVKNSPDIVFIMGGTNDFIAGAGNEIINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNVREDWSCFTDFNIVSQNLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            12495..14192
                     /locus_tag="PC545_001085"
     CDS             12495..14192
                     /locus_tag="PC545_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2455873.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKLELENIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETSRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSTIPTLTEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNVPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTTGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYDGLIELYKK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSIKVVDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVEKLK"
     gene            14406..15245
                     /locus_tag="PC545_001086"
     CDS             14406..15245
                     /locus_tag="PC545_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDF2455874.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLVGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIRHE
                     MLK"
     gene            15762..17618
                     /locus_tag="PC545_001087"
     CDS             15762..17618
                     /locus_tag="PC545_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2455875.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYRVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIIGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGENTGKLFRDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIVDNIIYSGYSNRNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVENKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTARDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKMQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKKDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGIGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    17701..17941
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    18007..18242
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18327..19544
                     /locus_tag="PC545_001088"
     CDS             18327..19544
                     /locus_tag="PC545_001088"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2455876.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKDKKYSNIITNNIDDIFNKDIDILVEVMGGLEPSLSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            19661..20155
                     /gene="cdeM"
                     /locus_tag="PC545_001089"
     CDS             19661..20155
                     /gene="cdeM"
                     /locus_tag="PC545_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeM"
                     /protein_id="HDF2455877.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGCSDSGENRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     EAEKYWDEYSKYNYKGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            20420..21685
                     /gene="hisD"
                     /locus_tag="PC545_001090"
     CDS             20420..21685
                     /gene="hisD"
                     /locus_tag="PC545_001090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDF2455878.1"
                     /translation="MKSIKTSSMKEKVDEKILREQVSSIIEDIRNNKDVALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIREFAKAQKLSFENPFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKINPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSNPVYIAADLLAQCEHDLNARGILLTNSLEVAQKVEKNI
                     ESMLKDLPTKDIAYSSWENNGEIILVDDMEEAIKISNFYAPEHLEVAVNEYNDICDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEKAI
                     QSLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(21888..22640)
                     /locus_tag="PC545_001091"
     CDS             complement(21888..22640)
                     /locus_tag="PC545_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2455879.1"
                     /translation="MSYFQYQGNSCFYKEYGQGKPIIFLHGNTGSSNMFKALVPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDIWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCHGKDWEN
                     VVNLDTKALLQCANEKRPLFHKELCKLEMPILFTGSKEDEMCRHNLEEEYKQMATFIS
                     KASIHIFSQGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(22758..23027)
                     /locus_tag="PC545_001092"
     CDS             complement(22758..23027)
                     /locus_tag="PC545_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455880.1"
                     /translation="MSYQNSIDLLPKELIEQVQEYIDGKVIYIPKKQEHKKHWGENTN
                     TKQVLASRNSQICINFQNGMSIKQLSEKYFLTEKSIQRILKQQNL"
     gene            23502..24596
                     /locus_tag="PC545_001093"
     CDS             23502..24596
                     /locus_tag="PC545_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2455881.1"
                     /translation="MKNIVGKPQTIKKVNEDLIKNIIKNEGPITKPEISNITELSLAT
                     VNKIVEQLALKNEVKVSGLSESTGGRRAQLFEINANLEHIIALYYYRNCCIGVVANLL
                     GEIIYQEEFNIRMDTYENVNQDTFFVIDTLIKKEKGNNIRAIGIGVPGVVKKGVVKNI
                     PTIKSWEGINLENLIKSKYNTKVFIENDTNITTIGIYQKQYKDKYKNMALMYLEDGIG
                     SGIVINGELYLGSTNFAGELSYLNIEFGKYNSNNLNLEDYIMSLRKRYLESKDNKFKK
                     EILSIISNFIRTLVCVLNPQAMIIQCSILTKSDMEFIEKDVSNNVGEENFPKLIKVES
                     LREGSINGVISMCLKETDYQYSLSNKKGGY"
     gene            24598..25548
                     /locus_tag="PC545_001094"
     CDS             24598..25548
                     /locus_tag="PC545_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDF2455882.1"
                     /translation="MKYLVSIDGGGTKTKFCVSDLDGNILKEHTTGSTNYKSVGIKKT
                     YENINNGFKKILKDLYIDYDDIEYTVFGISGCDSPNDYKIIMDEILKIGINKEKIYLA
                     NDAVLAFYAQADSPGLVIVAGTGSIILGIKEDGEIYRVGGWGYNFSDLGSGYDIGRKL
                     LKKVLLYCDECHEYSDLFSCVLDFFGANSFEQLAYMITDINNNVEIANLASLVIDCAE
                     QGDKLAIEILRESSTELSKLAQVILRKISNPMKVDKSEINIVLSGGTLNKTVYAQMLM
                     NNLKTENTDKNLKFISQENQPVYGGIRLAMALANRRVKHG"
     gene            25541..26725
                     /locus_tag="PC545_001095"
     CDS             25541..26725
                     /locus_tag="PC545_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455883.1"
                     /translation="MDRKKVIVQIFTGGFKAKEIKFNDIKCKLLELLDCIDIEKVIMG
                     WSVDEELYVETKKLLNKYNVELYLWLPCFSEIGLLEKSNLLIDYMGKEVKGYALQEDE
                     NFEFYCPNNKENIKNIKKIYSTYFSDIKFDGIFLDKIRYGSFSNKLNGVFNCFCKDCL
                     NIYEKNNIDKESLMREMNNVSKGVIDNEDTPFGITEYINGKYKFKNKIWEDFFETKNQ
                     NIYKALLEISNYFKSKGLKIGIDTFSPFISYFTGQDLEKLQEVADFIKPMMYRTTKAP
                     AGLPFEFERFIDESIHKNKEIGKRNFLDILGIKEYEGDVYPIEFVLEEIDFMTRNLNK
                     DIYVGVEINKKEIISPVTPKYISENISNINKTTVKGYVLSWDLLSAPKENIDEIIKWF
                     RN"
     gene            26738..28234
                     /locus_tag="PC545_001096"
     CDS             26738..28234
                     /locus_tag="PC545_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2455884.1"
                     /translation="MSKILEMQQIYKSFGKVDVLKGINFELEKGEVRALLGANGAGKS
                     TLIKILGGVHSQTKGKVILTGNEIMFKNPHHSKENGISIIYQELSLIPTLTVLENIFL
                     GREILKGSVFLNKKDMLEECMKICEDFDFNIDPHMVVSNLSVANQQMVEIMKAVSCNS
                     EIIIMDEPTTSLTNNEKDGLFKIISRLKSKGKTIVYISHILEEVFKVCDSASIMKNGI
                     MIGTYDVEDLTKLKITQLMTGNDNHKLIEKKDYTFAKYEEPPVLEVKNISWSNKIKNV
                     SFKVYRGEVVGLAGLVGSRRTEIINLIFGIDNKDSGDIFINGKKVDITSPKVAIKNKI
                     GLIPEDRKNLGLILEQAIYTNSSSVQIDKFKKFLFIDRNKEIEFSKKGVKELGIKVND
                     VKQEVKELSGGNQQKVVVSKWLEKDLDLIIYDEPTKGIDIAAKEDIFNTIKEFSSKGI
                     GVIFISSDLEEVIRVSDRILVTREGSIVDDLKNENIQVQDIMNSIFNV"
     gene            28257..29207
                     /locus_tag="PC545_001097"
     CDS             28257..29207
                     /locus_tag="PC545_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455885.1"
                     /translation="MKKAFEKLNLRDYGVLLGFLALCIAISIATPSFLGKQNILNLLR
                     QSSIIGIISAGMTFVIISGNFDISVGAVAALAGAITMKFATGGTNLFASMLLGVAICA
                     VIGLINGIVVAKINVPSLIATMAMVTIVRGLLLMLTGGYPITENIPILDYIGNGYLFN
                     IPIPVVIFFIVVLASFIVLNKTKFGRYVYSVGGNQEASKLNGINVDSHKVKVFIINAV
                     LAGIAGIVLTGRLGTATAIAGEGYDMDAIASVVIGGTSVAGGSGSVLKTVIGVLLMSV
                     INNSFNLLGIDVYFQYIFKGLIILAAVGFDSYSKKKLASR"
     gene            29228..30190
                     /locus_tag="PC545_001098"
     CDS             29228..30190
                     /locus_tag="PC545_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2455886.1"
                     /translation="MKKLMKHLALLLSLVMIFGLVGCSNGGDKEKNGEKKIAVLLPGS
                     TGYFVATKQGIDAKSKELGVSVEYADAQWDASKQLSQAEDFMAKGVDMIALCGVDSAV
                     SERIVKAANDSDVPIVAFTNSIGSNPTGEFKGLVTYIGQNEEETGALTGKIAKNLLGE
                     TGGKAVLIEGVPGTTPQVLRKKGLEKELKDSNIEIVYNQTSNWEKEQALKVTEDLIQK
                     KTDFNVIICQDDNSATGAGQALKDAGLKDKVKVIGLGGSKDGLKAITDGLIDGTTYMS
                     AVEEGGLVIEKASKFLKGEKIEPVTQIKQVEVNKDNISEFKGEW"
     gene            30562..31194
                     /locus_tag="PC545_001099"
     CDS             30562..31194
                     /locus_tag="PC545_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2455887.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            31605..31697
                     /gene="kdpF"
                     /locus_tag="PC545_001100"
     CDS             31605..31697
                     /gene="kdpF"
                     /locus_tag="PC545_001100"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDF2455888.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            31738..33420
                     /gene="kdpA"
                     /locus_tag="PC545_001101"
     CDS             31738..33420
                     /gene="kdpA"
                     /locus_tag="PC545_001101"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDF2455889.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYAAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGLILATQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGHMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTTVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAIPAGLEGISNPGF
                     HGLSQVLYEFASSAANNGSGFEGLGDNTMFWNITTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            33445..35502
                     /gene="kdpB"
                     /locus_tag="PC545_001102"
     CDS             33445..35502
                     /gene="kdpB"
                     /locus_tag="PC545_001102"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDF2455890.1"
                     /translation="METKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRAYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFIAVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGTVPEDLEENVNRVAKLGGTPLVVCVDNEIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQLE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            35521..36147
                     /locus_tag="PC545_001103"
     CDS             35521..36147
                     /locus_tag="PC545_001103"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDF2455891.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELHDRVQKDIEQFLKDNPTVKREDIPTDLLTASGSGLDPNISPASAKIQIPAI
                     AKASGISESKLQKIVEDNTSKKLFGVLGEDRVNVLKVNVEVAKILGLI"
     gene            36384..37292
                     /gene="cysK"
                     /locus_tag="PC545_001104"
     CDS             36384..37292
                     /gene="cysK"
                     /locus_tag="PC545_001104"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDF2455892.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEQRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSVER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIRVIALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEVILVDDEDAFDTVKLLAAKEGILVGISSGANIFGAIEM
                     AKKYPDKKIVTVAPDGVDKYMSMGIF"
     gene            37319..37909
                     /gene="cysE"
                     /locus_tag="PC545_001105"
     CDS             37319..37909
                     /gene="cysE"
                     /locus_tag="PC545_001105"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDF2455893.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVSYEYDELDNVYYI"
     gene            38069..38257
                     /locus_tag="PC545_001106"
     CDS             38069..38257
                     /locus_tag="PC545_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2455894.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMKDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(38311..39180)
                     /locus_tag="PC545_001107"
     CDS             complement(38311..39180)
                     /locus_tag="PC545_001107"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HDF2455895.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEIGMSTEDINKLVHDFTLQNGAIPAPLDYNG
                     FPKSVCTSVNNEVCHGIPSKDIILKDGDIINVDVSTIFNGYYSDASRMFMIGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDVINSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(39377..40621)
                     /locus_tag="PC545_001108"
     CDS             complement(39377..40621)
                     /locus_tag="PC545_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455896.1"
                     /translation="MENNLNTAKRNNTELLQNDSLKVNLQIGVDFTLLSKSLKAFYKK
                     EKDFYCIALAPCNVSSEKANRKISIYNMIKEINILIESITDKNELLSEELFTNNLSEF
                     YDESLDEIYIDLKQAYLYIKKNTDSLNIEDSIEYVFDVDISNDIKAKENPLINFNSLS
                     LVVWSGETKHILQQMNILDLEDIHSYSIENNTNITIYENNTLQYKFDEESENIKDSSI
                     DIIEQMLILPDDLNYDKDEVENMKNRLAKINIKYLQTLKEKDIKIKLINSNLTDEPEF
                     SDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYANVHSSKNLELHETAHA
                     IDKNVLNKKSNSEEFMEVFAQERYKLYDPKQVAHAYISKFIEEFFAESFVHYYLDEDS
                     KNTLKENCPLTYDFLEKLELNY"
     gene            40912..41223
                     /locus_tag="PC545_001109"
     CDS             40912..41223
                     /locus_tag="PC545_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2455897.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGINSKYTRAKGFE
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPSILINLIDNKEDYIIGKEIEQL
                     T"
     regulatory      41316..41408
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41508..42302
                     /gene="thiD"
                     /locus_tag="PC545_001110"
     CDS             41508..42302
                     /gene="thiD"
                     /locus_tag="PC545_001110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDF2455898.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGEEILQLGPKFVLMKGGHLDGEAVDILVGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNLSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            42326..43114
                     /gene="thiM"
                     /locus_tag="PC545_001111"
     CDS             42326..43114
                     /gene="thiM"
                     /locus_tag="PC545_001111"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDF2455899.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANVTLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEEDSTEIIRKIAEKLECVVV
                     ATGKIDIITNGKGTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLT
                     MSLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            43137..43769
                     /gene="thiE"
                     /locus_tag="PC545_001112"
     CDS             43137..43769
                     /gene="thiE"
                     /locus_tag="PC545_001112"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2455900.1"
                     /translation="MIDKESLKKCLKLYLVTDSEMLKGRDFYKCLEDAISSGITTVQL
                     REKNASGREFLRKAMKLREITKQYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNNKILGVSARTLEEAICAKNDGADYLGVGSIFTTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLESDGYAIISAILKAEDISKEVEKWTLKI"
     gene            43908..44210
                     /locus_tag="PC545_001113"
     CDS             43908..44210
                     /locus_tag="PC545_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455901.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMLDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(44276..45025)
                     /locus_tag="PC545_001114"
     CDS             complement(44276..45025)
                     /locus_tag="PC545_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455902.1"
                     /translation="MLNILWRSMKWRFKNPISFVVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIVLVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVTILCIVSIFFSVRIESGIGGILLMIVLIFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIESLSGGLKVAVMLNPFTHVINALRSLIFGETILI
                     GDILPVILLFLILCCSSFSLAMWRLKKEMVS"
     gene            complement(45019..45981)
                     /locus_tag="PC545_001115"
     CDS             complement(45019..45981)
                     /locus_tag="PC545_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455903.1"
                     /translation="MLAIEIDNLVKEYKNGVKALNGLSFNVNAGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMVFQS
                     KMYKVEPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRDEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNILRITFHNTEDIKKYKDSIKSTGLVKFMSVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEEWEC"
     gene            complement(45985..46443)
                     /locus_tag="PC545_001116"
     CDS             complement(45985..46443)
                     /locus_tag="PC545_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDF2455904.1"
                     /translation="MNCKIEIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNG
                     APFFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHTGSYSNLPKT
                     YEMIFKYIHENNIKIQTPWREVFIKGPGMLIKGNPDNYITEIIFPLKEEE"
     gene            46799..47155
                     /locus_tag="PC545_001117"
     CDS             46799..47155
                     /locus_tag="PC545_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455905.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVTGELKPGETIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNYQSSVTVDENILKNLKLELINESLSVFIEDMKA
                     INVSKEEVLKIIEDKY"
     gene            47227..47943
                     /locus_tag="PC545_001118"
     CDS             47227..47943
                     /locus_tag="PC545_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455906.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINIDSGEIRIDGEKINEKVYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKEKSKEMMDIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVVILDEGQIITSFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            47936..>48623
                     /locus_tag="PC545_001119"
     CDS             47936..>48623
                     /locus_tag="PC545_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455907.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLSLNLFEINKFKNS
                     KFIMNLAIEDFGGIFYGLGILSNLGFIQSILFFGILGLFIYYVFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALITMIYGFLISQTATLFICKYIFNFIFRNMPIINMNFAK
                     DLYYLNLKSIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYVILITLLIIAG
                     LYFSTIYINII"
BASE COUNT        18877 a         5174 c         8513 g        16059 t
ORIGIN      
        1 actgaaaatt ctaaaattat aattgtaaga ttatataaat ttagaattta aatctgttga
       61 ataatattaa aacaatgata atgagtatga atttctaata aaaataaata tcataaaaat
      121 aagaaaaagt attgggagga aatgaaaatg gcaagaatgt attatgaaaa agatgtggat
      181 ttagaggttt taaaaaataa aaaggttgca gttttaggtt atggaagtca aggacatgca
      241 catgcacaaa atcttagaga taacggtgta catgtagtga taggtcttta tgacggaagt
      301 aaatcagcac aaaaagcaaa agaagatgga tttgaagtta agagtgtagc agaagctaca
      361 aaagaaagtg atttgacaat gatgttaatg cctgatgaaa agcaaaagaa agtttatgaa
      421 gagagtgtaa aggataactt aaaagaaggt cagacattag cttttgctca tggattcaat
      481 atacattata atcaagtaca accaccagaa tttgttgatg ttgtaatggt agctcctaaa
      541 ggacctggtc acttagttag aaatgtattt acaaagggaa gtggagttcc tgcactattt
      601 gcagtatatc aagactacac taaaaaagct acagagacag ttttagcata tgcaaaaggg
      661 ataggagcaa caagagcagg tgttttagaa actactttta aagaagaaac agaaacagat
      721 ttatttggag aacaatcagt actttgtggt ggaataagtg aacttataaa attgggatat
      781 aaaacgcttg tagatgcagg ttatcaaaaa gaagtagcat attttgaatg tttacatgaa
      841 atgaaactaa tagtcgacct tatatatgaa ggtggatttg aaagaatgag atatagcata
      901 agtgacacag cagaatatgg agattatgta tctggtaaaa gggtaataac tgatgctgca
      961 aaacaaggta tgcaaaatgt tttagaggat atacaaaatg gaaaatttgc aaaagcgtgg
     1021 attaaagaaa atgaagaagg acgtgaaaac ttcctaaaaa ctagagaaga agaatataat
     1081 acagaaattg ccgaagttgg tagaaatcta agaagtatga tgtcattctt aaaataaata
     1141 tatattaaat ggattagttt gagggaaaga gtggtgtata gtagtgcgta tgagtggagc
     1201 aaaggtaatt ttagaatgtt taaagaaaga gggaattgac acaatatttg gatatcctgg
     1261 tggagccgtg atacctcttt atgatgctct atacgattat tcagatgatt ttaaacatat
     1321 aaggacatcc catgaacaag gtttagttca tgctgcagat gggtacgcaa gaagtactaa
     1381 tacagtagga gtttgtttta caacatctgg tccaggagca acaaatgcca taactggaat
     1441 agcaacagct tttatggatt cttcacctat ggttgtaatt tcagggcagg tgccaactag
     1501 tttattagga aaagattctt ttcaagaaat tgacataact ggagcaacat tatctatgac
     1561 aaaacataat tatttagtta gaaacactaa agaattagtt cctacaataa aagaagcttt
     1621 tagagttgca aattcaggta gaaaaggacc tgtacttgta gatgttccaa aagatttatt
     1681 tttagcagaa atggatttta gtggtgagga ctatgattta tgtcaaatag atgattatat
     1741 ggattataag agtgattttg atctagatga tgaaactaat attaagcttt taaatgaagc
     1801 aatagatatc ataaaagaat ctaaaaagcc tataatatat gcaggtggag gtgttaaatc
     1861 ttcagacagt gaggaaattc ttgaaaaatt tgcaactaag atagatacac ccgtactaaa
     1921 tacacttatg gggcttggaa atatagatag aaagaatgaa ttatctcttg gaatggttgg
     1981 aatgcatgga agtagagaaa gtaatttagc actttctaat tcagatttag taatagcgat
     2041 aggtgcgaga tttagtgata gagtgataag taaaagttct gaatttgcta aaaatgcaaa
     2101 aataatacac atagacatag acccatctga aataagtaaa aatatagaat ctaatgtatc
     2161 tttggttgga gatgtaaagc tagtccttag tttattaatt gaaagggttg aaagtaagaa
     2221 taatagcaat tggaaagaag agataaagag atttagaaag agtgagggtg tacaaacata
     2281 tgaatttcat ccacaaaata tactaaaaaa aataaatgaa aaatatgaaa cattaaagaa
     2341 acccactgtg gtagtaactg atgtcggtca acatcaaatg tgggctgcta agtactggaa
     2401 ttttaaaggt aataaaagtt ttataacatc agcagggtta ggaactatgg gatttggttt
     2461 aggtgcagct attggaacta aagttggtaa tgtagataaa aatgtagttt tagtcactgg
     2521 tgatggaagt tttagaatga actgtaatga actagctaca gttgcaaatt ataatgtacc
     2581 tatgcttatt ttattactca ataatagaac attaggaatg gtaaggcaat ggcaaaagtt
     2641 attttccaat caaagatact ctcaaactga cattaatgaa aatgtggatt atgttaaact
     2701 cgtaaatgca tataatattg atggatataa ggtatccagt atggaagaat taggaaaggc
     2761 gctagatatg atagatttta ataaacctgt atttttgcaa tgtgatatag ataaggatta
     2821 tgatgtttat cctattgtag cacctaatga tgcattggaa aatttgatat gtaactaaaa
     2881 cttatactga atattgataa aattttttat taaattttta ttggtattca gtttttgttg
     2941 tttagagata gattggcatt ttttattttt gcaaataaat caatgactat ataattaaga
     3001 acttattaaa ttttaattat acaatttaga atagtattac aacttaagta aataataatt
     3061 ttgaataata atttacggag gtattcatat gtttaaacac gataaagcat tattaaaaga
     3121 agtaaaagta gaaagaccaa atcctcaata tgctgtttta atgcaagaac agttgggtgg
     3181 tgcaaatgga gagttaaaag cagctatgca atacttgtcc caaagtttta gaataaaaga
     3241 ccctcaaata aaagatttat ttcttgatat agcagcagaa gagcttagcc atatggaaat
     3301 ggtagcacaa actataaatt tattaaatgg acatgatgtt gattataatt cagttaatac
     3361 aggcgaaata gaaacacatg tattaactgg tctatcacca gtcttaataa actcatcagg
     3421 agctccttgg actgctaatt acgttacagt tacaggggat ttagtagctg acttactttc
     3481 aaatatagct tctgaacaaa gagctaaggt agtttatgag tacttgtata gacaaataga
     3541 tgataaatat gtaaaagaaa caatagattt cttacttaat agagaagaag cacataatgc
     3601 tctatttaga gatgctttaa ataaagttaa agatacaggt tcaaatagag attttggagt
     3661 tacagaagat tctaaattat atttcgattt gtctactcca gggccaaatc acgatactaa
     3721 gatagatatt aatcctcctt cttttgaaaa acctttaaaa aaataatttg atatttttta
     3781 tgttgttttg ttttagtagt tataaaaatt atttactagg agaattaatt atatgaaaaa
     3841 ttaaatatgt atggttaaaa atgggtatac acttattata agtgtatatc tatttgttta
     3901 ttgcaaaaca tataataaaa taacaaatga ggtggtattt tggacagtaa tagaaaacct
     3961 aataatctaa taaatgaaaa atctccatat cttttacagc atgcatataa tcctataaac
     4021 tggtatagtt ggaatgatga ggcttttaaa aaagcaaaag aagaggataa gccaatattt
     4081 ttaagtgtag gatattcaac atgtcattgg tgccatgtta tggaaaaaga atcttttgaa
     4141 gatgaagaag ttgctgaaat aatgaataga aactttgtgg cgataaaagt agataaagag
     4201 gaaagacctg atgtagatag cgtgtatatg acagtttgcc aagccatgac aggtagtggt
     4261 ggatggccaa tgaccataat aatgacacca gataaaaaac ctttttttgc gggaacatat
     4321 ttcccaaaat attcaaggta taatagacct ggagttatag atttattaga gaatgtatct
     4381 gaaaagtgga atacaagcag ggatatatta ataaaatctg gagatgaaat aatagaagct
     4441 cttaaggatg attttggtgt aaaaaatact gagggtgatt tatcaaaaga gatgttgagt
     4501 tcatcagtta gagtatttaa ggctatttat gatgaaaagt atggtggttt tggtaatgct
     4561 cctaaatttc caagtcctca gaatcttatg tttttaatga aatattatag tatagaaaaa
     4621 gacaaagatg tgttaaaaat ggtggaaaaa actttagatg gaatgtatag aggaggcttg
     4681 tttgatcata ttggatttgg gttttcaaga tactctactg ataaaaagtg gttggctcct
     4741 cattttgaaa aaatgctata tgataatgct atgcttacta tagctttttt agatgcgtat
     4801 aaaataacaa agaaagagtt atataaagaa attgctataa aaactataga ttatgtagtt
     4861 agagagatga aggataaaga aggtgggttt tattcagcac aggatgctga tagtgaaggt
     4921 gaagaaggta aattttatac atttaatcct cttgagataa ttgaagtttt gggtgaagaa
     4981 gatggaattt tctttaataa ttattttgat ataactagta gtggaaattt tgaaggcaaa
     5041 agtattccta atcttataaa aaataaagaa tatgagagac ataatgaaaa gatagctgac
     5101 cttagtaaaa aggtatttga atatagaaaa gaaagaacat ctttgcataa agatgacaag
     5161 atacttacat catggaatgc attgatgata gttgctttaa caaaggcata tagtacttta
     5221 aaaaatgaca tatatttaga gtattcaaat aagtgtctta actttataaa taataatctt
     5281 gtaaatgagt caggtagatt attagctaga tatagagatg gaagttccga ctatttagca
     5341 tatttagatg attatgcttt tttaatctgg gcgtatatag agctttatga gtcaacattt
     5401 aatatgaaat atttagaaaa ggctttaaat ttgaatgaaa gctgtatcaa ccttttttgg
     5461 gattatgaaa aaagtggatt ttatatatat ggtaaagata gtgaaaattt aatagcaaga
     5521 ccaaaagatt tgtatgatgg agctattcca tcaggaaatt ctgttcaatt atacaatctt
     5581 ataagacttg cgaaaattac tggagataat agacttgaag aaatgtcata taaacaatta
     5641 aaactgtatg tggataatgt aaagagttct ccaactggct acagctttta tatgctttca
     5701 ttgatgtttg aactttattc tactaaggaa attatatgta tttttaagga agattctgat
     5761 ttaattgcat ttaaagaatt gataagtgaa aattttatac caaatgcaac ttttttagca
     5821 aagaaataca atgaagaaaa cactattata ggttttttaa ataactatat attgaaggat
     5881 gataagacta gttattacgt gtgtcaaagt aatagttgta gtcaaccaat taatgattta
     5941 caaaaattaa aagatatgat tttaggagta gaataatcat aatagcgaaa aaggagatgc
     6001 tttaaaataa aagtctaatc atctcctttt tttgtataaa aaacagaaaa agagttccta
     6061 agaacaccta atctatgtaa tgattgtttt gttaaaaaat aaaaatgaca ttatagttat
     6121 ataaaatatt gtactataaa aaagtggtat tatagatgcg atagtattaa tcttttttat
     6181 ttaccctatt tcttcttttt gcaaattctt caagtgcttc agatttaatt ttatctattt
     6241 cttcatattc cataaaatta gcatcttctt caaagccatc ttgttcttca ccattaaaac
     6301 gatacatttt ttttagtaaa ctccataagg tctctaattc atctttggta aattcgtgaa
     6361 atacatctgc cataaagttt attccagtcc tactacatgt taccataacc ttttttccta
     6421 agtctgtaac tttaacatta attgctctct tatcatgagg acttggaatt acatcaacat
     6481 atccattttt ttcaagatta gcaactagtc tatttatatt ttgtttactt gtacccattt
     6541 ttcttgcaat attatttaat gttgtttctt cctctggtaa atgaagaatt gataaaatag
     6601 tcatatactg tcttgaagta agtataccaa aatatttatc tccttctatt tgtattttgt
     6661 ttgtaagggt aaaaagagtt gcatatgttt gattcattaa aaagagctct tttagttcat
     6721 ttgaataatc catgaaatat atctccttta tttttagatg ttgttttgtc gaaaacagaa
     6781 tatcatgaat taattctaat gtcaatatgg tgtcaaaata tattaaagta ttactttttt
     6841 atgtaactat taattaatta aattattgta taattttatg taaaaacttc tatgtaccaa
     6901 tgataatttc tatgaggaat taataattct taaatttttt agaaagattt tgacttgaaa
     6961 atacttgtaa tataatatat aaattaatat actgaataaa atcaatctgg agggataatt
     7021 atgaagtgta caaactttac tttcaaagga gaggaaggtt tagatatata tacatataaa
     7081 tgggaagatg aaaatataaa aaaaccaaag gcagttatcc aaattgcaca tggaatggct
     7141 gaaactgcac aaaggtatga gacttttgcc aaggtactta ctaaaaatgg ttatatagta
     7201 tatatcaatg accatagagg tcatggaaaa actgcaaaga taatagaaaa tgttggacat
     7261 ttagctgaaa aagaaggatt tagatgtctt gtagaggaca tgtatacttt aacaaatatt
     7321 ataaagaaag aaaatgaaga tttgccaatt tatctatttg gacacagcat gggttcattt
     7381 gcaagtcaaa gatatataat ggattacagt aataatttat ctggtcttat actatgtggt
     7441 tcaaatggaa agcaaggaat cattctaaat cttgctcatc tgataatcaa tcgtgagatt
     7501 aaaaagtatg gaagacgttc taaaagtaat aaaataaata atttgatatt tggtggcgaa
     7561 ataataagaa gaaatgaaaa gactaagttt gactggttaa gtagagataa agaacaagtt
     7621 gaaaaatata taaatgaccc attttgtgga gtagtgtgta gttgtggctt tttttatgat
     7681 ttagttcaag ggttaaaaga aattgaggat aaagaaaact taaaaaaagt gccacttgat
     7741 atacctattt acataatatc tggagataaa gaccccatag gaaaaaatgg taaaggagtc
     7801 ttaagactaa gggatagata tataaaatta ggagtaaaag atgtaacttg caaactttac
     7861 aaagatggaa ggcatgaatt gttgaatgaa ataaatagag aagaagtttt tgaggatata
     7921 atttgttggt taaataataa aatagaaata ttgtaataaa aaaatataat gatatatttg
     7981 ctttaaattt atttatatat aatatttgaa aagtggatta tgtatatgca aagtagaaaa
     8041 gagagagcct agaagtgatt ttttgctata atgccattta ttgaataggt gggtgttatt
     8101 ataaataaat tattaacact cagtatgatt atcattgtat atggaattta ttttatttaa
     8161 aattccttga aagtttaaat atagtaagtt gtaataaaga gaaaatctaa atatttaaat
     8221 gctatttaat gtgaatctat taattaggtt atacttaaac ttaattaata gattttatat
     8281 ttcaagccaa tgagataggg ttttagatta aaatataacc aatggttaaa ttacagacat
     8341 tttgttaact ttccctcgat ttactagtaa atattttttt tatatactta aattacaaca
     8401 gatgtactag ggaggtatat atgcaaatta aaataggtaa agtcatacag tgtcttagaa
     8461 aagaacgaaa tttaactcaa gaacaattag caaagtttat aggtgtgtca acaccagcag
     8521 tatctaagtg ggagagtgga aattcttacc cagatataga attattgcct ttattagcag
     8581 acttttttaa tgtgtctatt gataagcttt taaattacaa gattgattta agtgaagaag
     8641 aagtaatgaa aatttataaa gaattagaat caggctttgc tagaattgaa attgatttat
     8701 caacagaaga accaaaagaa gaatttagac aagacttaga atctgtaaaa aagctttcca
     8761 atatgtatat agagaagtat cctaaaagct atttattgaa gctaaggata tgttcgctgt
     8821 atcagatgta ttcatataaa tttggtaaga gtgaacttaa tgacagagta aaagaaacaa
     8881 tgaatattct tgaagatata gtgagaaata cagatgatat ccaaataaaa gaaacagctc
     8941 ttattatatt atcaaacgca tattgtatgt tggaggatta tgaaaaggca gagttgtatt
     9001 taaatatgat acataagtca ataggggata caagtgttaa tttagctatg atatatttaa
     9061 agcaaaatag acttgaagaa gcagaaatat tgcttcaaaa taaattgttt agtaatgtat
     9121 ttaatataag catggattgt aagggaataa ttaatgtata caaaaatcaa tacaaggaat
     9181 taaagaaaaa attggagaat agaaatttta ataaaaatgc aattgagaga gaaatggagt
     9241 atataaaaaa taagttgctg gggtatgcaa atctttcttt agaaataaaa aaaatgctca
     9301 gtgaagataa aggtgcattt tttagtatgt atatggatta tatggaatta tctctaattt
     9361 ttttattttt taacatgaaa gaagaagcaa aaaaagcctt atatagtcta aaagaaatat
     9421 tagaaaaata tcctatacat gaaagtttgg atgtaagtca aatgagattt tttgataagg
     9481 tagagtctaa aaatttatat acttttaata tatatacaaa cttgttaatt gtacttaatg
     9541 atgatagtta taatgagctt agagaagaac caatttttaa ggatgtaatt gaaaagattt
     9601 tagatatgga aaaaatgttg aaaaataaag aataagtaaa aatttaatat aggacttaaa
     9661 gattaatcaa attaaaagaa agtgtttcaa aataaaaatt tgaatcacct tttttgtcgg
     9721 tcttaaacag ttatttagaa aaatagaggt ttcaaaatag aaatctgaat tatctgtatt
     9781 attacacaaa aatagaaacc taaatttata aaaatagaat aaagtttgtt atataaaatg
     9841 taattttgtc tataaaatct cacttgtttt cttaacttta agcgtgaaaa agagaatgta
     9901 tcatgaattt tttagttcat gatgcatcct tctttgtatg aaatccaata tatctttatt
     9961 atttaaacaa tggaaaagag ccttatttct attttgagac atctctttta tttaattatg
    10021 aagttttata cagttcaata aataggaatt atatttagaa actcatgaca ttatttctta
    10081 gatagtgatt aaatttatca tattccattt tagataactt ttctgggtaa gacattacaa
    10141 tcaggttatg cccattaaat ttttcatggt atggtggatg aatgtgttca taattatttg
    10201 tcttatattt tactctttca tagaacgcct tgcgataaat tgaaatctca tcaattggaa
    10261 ggtcaatttc taaaataata gtttttcctt tcttatttaa aagctctaac gctctttgtc
    10321 catatctatt attgcgcatt tctggaaaaa tgcagaaatg ttctacatag ataaagtcat
    10381 ctgtttccca acataaaatt atgccgacaa actggttttt atcataaatt aaattgaact
    10441 gatattcttt atttttcaaa atttcttctt gcaaagaaga ttttctttgt tcatgaaatg
    10501 ggaaactaat tttatataat tccatagcct tgtaacataa tttattttca ctggttctta
    10561 accgtttaaa tttcacttta ctcctcctaa tcacgaaaat tccaatttat ttatgagttc
    10621 attttaccat agtcagtatt tgcatacaat gtgttttaag ttgagtgtca tttctttctt
    10681 ttatttatgc ttatatctca tagactaaga gataattgct taagacataa ctaaaaacta
    10741 ttatattaag ctttgataat ctaaaaacag tatatttatg gacttttcta ggtctaaaag
    10801 taatcttaat aattttagaa gttacaaaaa agttacataa atttggaaag ctataatata
    10861 catatataat ataaggttaa aactgtaatg aataatatta atttaaaatt tgaacatgga
    10921 aggagattat tatggagaaa agagaaaaga ggataataat atcatcttta ttaagtgtat
    10981 caattttaat gggtttggta agtatatatt ctatattaaa taaagaagac ataattttaa
    11041 ctgttaaggg tcaagagcaa aaagtatctt cctttaagaa aacagttgag gaacttttgg
    11101 atgaacaggg tataaagtat aattctgaag ataagattaa tccaagttta gatacagaac
    11161 taaaagatga tatgaaaata aaagttgtta aggtaactaa aagtaaaaaa gaagagattg
    11221 aaaaaattcc atttgataca aagcatgtaa atgatagtaa tttgttaaaa ggaaaatcta
    11281 aagtttatca agaaggtcaa gaaggagaaa aaaaactagt ctataattta acttaccatg
    11341 atggaaagtt agtcaaaaaa gtcttatcaa aagaagtaat atctaaggag ccaactacaa
    11401 aaattataaa atatggaact aaagaaaaag tactaatagc atcaagagga gcaaatataa
    11461 gaggaggcaa acatatgaaa gtggttgcca ctgcatatgc aggagataca ataacatcta
    11521 ctggtacaac tccaagatgg ggtgttattg cagttgaccc acgtgtaata ccatatggaa
    11581 caaaagtata tatacctaaa ctgggtatga cttttgtagc agaagattgt ggaggtgcaa
    11641 ttaaaggtaa tagaatagac atttttatga acagtgaagg aaaagcttca aattggggaa
    11701 ggaaaagtat agatatatat ctacattaaa ttagtgaata gaataacttt acttgaagga
    11761 caaaaaagta taaaatatga attaactaaa ctgttagatt attcttaaaa cataaaaata
    11821 tatctaaggg gggataagat gaaaatagta tgcttaggag acagtttaac gtatggattt
    11881 ggagtatcac gaagtaattc atggacaaac attgtaaata aagagactcg attagaaata
    11941 gtaaataagg gtataaatgg tgatacaaca agtggtatgt tagctaggtt taatgaagat
    12001 gttgtaaaaa attctcctga tatagttttt ataatgggtg gcaccaatga ttttattgct
    12061 ggtgcgggaa atgaaattat taattctaat ataatggcta tggtgcatca gtcatattca
    12121 aaaaatatta tacctataat aggtatacca ttaaaaccag atattccaaa tgttagggaa
    12181 gattggagtt gttttactga ttttaatata gtatctcaaa acttagaatc atatagttat
    12241 tggataaaaa aattttgtat gacatttaat actaatttcg tagattttta ttcagaatat
    12301 aataaaaata tggaactaga aggatataaa aaattatatt ttgatgggtt acatccaacc
    12361 aaagagggtc atagaataat ggcagacatt tttattaatt caattaataa gtatatagaa
    12421 gagttataga ttacattaaa atgcttgttt atgcgtcagt tgcaataaat acatacagta
    12481 gagaggggtt tattatggat tatactaaaa cttatgaaga gtggataaag ggttcatatt
    12541 ttgatgaaga tacaaagcta gagttggaga atataaaaaa taatgaaaaa gaaatagagg
    12601 atagattcta taaggattta gaatttggga ctgcgggtct tagaggaata attgaagcag
    12661 gaacgaatag aataaataag tacacagtta gaagagctac ttttggattg gcaaactata
    12721 tattagaaaa cacaacaaaa gaagaaacaa gtagaggagt agtaatagct catgataata
    12781 gacacaagtc tagacaattc tgtatagaat ctgcaaatac tttggcagca tgtggtataa
    12841 aagcatatat atttgatagt ttgagaacta ctccagaatt atcttttgct gttagaagct
    12901 taaatgcaat cgctggtata gtcataacag caagccataa tcctccagaa tataatggat
    12961 ataaggtata ctgggaagat ggagcacaag ttatgccaga gattgcaaat gcaataacag
    13021 agaaagtaaa tagtatacat gattatagta caataccaac cttaacggaa gctaataaaa
    13081 atttggtagt tttgttggac gaatcacaag atactaaatt tatagaagca gtgaaaagcc
    13141 aaattataag aaaagattta gttaaaaatg taggtaaaag ctttaagata gtttatacac
    13201 ctctttgtgg aacagggaat gttccaataa gaagagcatt gaaagaagtt ggttttgaaa
    13261 atataattgt agttccagaa gaagaaaatc cagacccaaa ttttgcaggg cttgattatc
    13321 caaatccaga agagaaaaaa gctttaaata gaggtatatt actcgcaaaa gaaaaaggag
    13381 cagaccttgt aatagcaact gacccagatt gtgatagagt aggtgtagca gtaaaaacta
    13441 ctacaggtga gtatgcttta cttacaggaa atcaaattgg tggtatgctg acacattata
    13501 taatagaagg attaaaagaa aataataaat taaaagaaaa tcctacaata ataaaaacca
    13561 tagttacatc agaatttgga gcagatatag ctaaagcaaa taatgttgat gtattaaatg
    13621 ttcttacagg ttttaaattt attggtgaaa aaataaaatt atttgaacaa aataaaaata
    13681 gaagttatgt atttggatat gaagaaagtt atggttactt agttgggact catgctagag
    13741 ataaagatgg tgtagtttct tcattgttaa tatctgaaat ggcagcattt tactattcaa
    13801 aaggtatgag tttatatgat ggtttaatag aactttataa aaagtatgga tttttcaaag
    13861 aacagactat atctttgact ttaaaaggta tagaaggtgt agaaaagata aaagaaataa
    13921 tatcttattt tagagaaaat caaattgatt atataaatag tattaaagta gttgataaaa
    13981 aagattataa aaatggtata gataatcttc ctaaatctga tgttttaaaa tactttttgg
    14041 aagatgaatc ttgggtagct ataagaccat ctggaacaga accaaaactt aaattttaca
    14101 tagctgtaaa aggagctagt gatattgaag cagataaaaa gattcaaggt ttgaaaaagt
    14161 atatagatga catggtcgaa aaattaaaat aaaatattat tattttataa gataattttg
    14221 taataatatt tatttagctg aaaattataa tttactttca ttgagtagaa tatggaaatg
    14281 ttagtagaat tgttattttc agcttttatt tgtgttattt ttacttaaat aattatatta
    14341 tagaatgaat ctgagtataa ttaatattgt ataatgaaat taatttgata gaaaagggga
    14401 tatttatgtt tggtatagat aagaaattga ttgaatatat aattgcaaaa tccaattaca
    14461 gtgaattaag ttcaaagtca gcagaagtat ctttcttctt aatgttgtcc atatttcctt
    14521 ttttaatatt tacaataagt tctatagctt atataccgat tcttcattta aataagtata
    14581 tagctttgtt tagaaatatg atgccagaag gtgcttttgc tgtgttatct tcaatcatag
    14641 tttctgcaat agacaataga aacctgaaat ttttagcagt cagttttgta cttactatgt
    14701 ggacattttc aagagcagta aaagctctta ttaaaggaat gaatagagcc tataaagtta
    14761 aagaaactag gtcttttttt aaaattttat ctatatcatt tttatttaca ataatgcttt
    14821 tggtccttat ttttttatct atgatatttt tagtttacgg tgaaaaaata ggttatttta
    14881 tttttaatct tgtaggattg gatgagatat ttataaaaat atgggatatt ctaagataca
    14941 ctgtaggtat aataacaata atagtgatat ttacactttt atataaatac actccaaata
    15001 aaaagttaac tattaaagaa tctgcgccag gtgctatttt tgctactttt gcttggtttt
    15061 tagtatcatt tttatattct tattatacaa attactatgc taactatgaa gtaatctatg
    15121 gaagtatagc tgaaataatt gtacttatga cctggatgta ttttagtagt tggtctatcg
    15181 ttattggcta tgaggtaaat tcaagattgt attttagaaa gataagacat gaaatgttaa
    15241 agtaaagtat ttaaaataaa agagagtatt tagtattata aatgtaattg aattaataaa
    15301 gaaatattga catttaagtt aaaaagtcta gaatttttct gggctttttt tatttttagt
    15361 ataacttgac aaattttatt taatgggata gttttaattt ataatcatct ttaaaaagat
    15421 gactattgat aagtaagaga taaaattggt aagtatcaaa aaatatacta aataaaaatt
    15481 atagtcctaa tgagttaatt tctgtatttt caaataatgc agtatttagt catgtgtttt
    15541 tatatgcgcc tgtatttcta attagcaata tattagaata agctactgta attagattta
    15601 gaaaaaagcg acataaataa ttataaacaa ataataaatt acataaaatt aaatttattc
    15661 acaagtatat cttataaaat atgttataat taatagaaaa ttgtcatata attaattaag
    15721 tagtattatt ataaataaca ttgaaaggta gtggttagaa tatggatacc cataataaat
    15781 atgtaaattt tataaaaaat atacctgtac cttttttata ttgtagaatt gtaaagagac
    15841 aagaagatat agaatatcga gtagaatata taagtaaggg tatgggcaaa gtattacaat
    15901 tagaagaagg tatttgtgat aaaaatatac tagatgtatt gcctgtattt aagtctaaaa
    15961 aatatttcaa agagttgttc tcaaatgaag tggattgtat aaaaaggtac attccgacac
    16021 ttaaaaactg gataaatata aaaaaacaga taatagggga ttcatacata attttgtatt
    16081 ttggtaaaat tgtatttgat tatagacaga taatcgattc ctttgataaa aaggaaaaag
    16141 tagcttatat taaagacgaa gaaggcatat atatagattg tagtgaaaat ttaataccaa
    16201 tattaaataa caacattaaa acaacgaaag atatctttgg taaaaatgat atagaagtat
    16261 ggggagagaa tacaggaaaa ttatttagag atgattatag agaaggagta tctagtaaaa
    16321 aaagattttt acaaaatctt tttgaatatg aagaaacatt ttttatggtt gaaaaatatt
    16381 ttttgtatga tgaagacgaa cttttaggta ctatagggat agtagacaat ataatatata
    16441 gtggttacag taatagaaat tataactcaa aagatttaat gaagatgata gaacactcaa
    16501 ttccagagaa tatgttttat aaagatgtgt atggaaatta tattggattt aattcaggtt
    16561 ttttgaattt agcttgtatg aataaagaag aattattagg gaagaatagt tataaaatat
    16621 cagaagaaga agctttaata gataagatat ttgaaagtga taagggtgta gttgaaaata
    16681 aaaaagtagt tacatttgag ttaaatataa gtatgaatga tgaaaacaag tgtattgaga
    16741 ttacaaaaag gccgtttttt gatagctatg gaagtgttat tggaataatt ggaacagcaa
    16801 gggatataag tagaagaaaa agattagaag aggaaatgga taaaactagg atggaatttt
    16861 ttgcaaattt atcccatgaa cttagaacgc ctataaattt aatatcatct tcattacaag
    16921 tgatagaaaa aaaagaagca gatttaatag aatcaaatga tactttaaaa agaaatctag
    16981 gtataataaa acaaaatgga aatagaatat tgaggttagt aaataatgtt atagacttta
    17041 caaaaatgca atcaggatat ttagatttta aaccagaaga atctgatata attgcattta
    17101 tagaggagat atgtatgtct gtagctgatt ttgcaagcca aaataatata caacttacat
    17161 ttgatacaga aatagaagaa ttttcaatgt tatttgattc agaaaaattg gaaagaataa
    17221 tattaaattt actttctaat ggaattaagt acaataagaa agatggaaaa ataaatatat
    17281 ttttatatgt gaaagataat gtatttaata tgaaaatatc tgatagtggt ataggtatac
    17341 caaaagaaaa aatagataaa atatttaata ggtttgaaca gattgacaat gaactttctt
    17401 acagagttaa aggaagtggt attggacttt cattagtgaa atctttggtt gagttgcatg
    17461 agggaagtat atctttaaaa agtcaacttg gtattggaag tgaatttata gtttcattgc
    17521 ctgttagaag taaaaataat attgaaaaat ataatcataa aagagaaatc agcaatgaac
    17581 taagtaaaaa attagaaata gagttctcgg atttataagt ttaaatataa aaaataaaat
    17641 tttaaaaaag tattgacaat tgatttagaa gggtttaaaa taaattacaa cataataaat
    17701 aaaaaggcga tgaataggaa gagtaaatct aattcgatta ccagagagaa gagtccgttg
    17761 gctggaagac tttttaaatt agagggattg aaaactacct aggagctact ttctgaagtc
    17821 atctgcatta gtagatttta gtaagaatag tcgttagctg cgttaaagtg taaagtgagt
    17881 tttcatgaca tatgaaaact aaattaggtg gaaccgcggg aataatactc tcgtccttat
    17941 gttataagga tgagagtttt ttgttattta aaaatataaa cagtaatcat aataattata
    18001 gaaaatataa aagcaatgaa taggaagagt aaatttaatt cgactatcag agaggaaagt
    18061 tcacaggctg aaagactttc taagtttgag aaattgaaaa ctacctagga gctgtggtct
    18121 gaagttactt taaaaagttt tagtaagact aaccgttaac tgcgttaaag tgtaaagtgg
    18181 attttatcaa taaatgataa aatcaaattg ggtggaaccg cgagtataga ctcgtccctt
    18241 tatataggga tgagtctatt tttattgcaa taaaagattg aaaataaaaa aaaatcggaa
    18301 aattataaat ttaggaggaa aataaaatgg agaataaagt gaaaattgga gtactaggat
    18361 atggagttgt aggaagtgga cttatagata taatagataa taataaagaa aaaagaaata
    18421 tagaaatagt tggaatttta gttaataatt tggaaaaaca taaggataaa aaatattcta
    18481 atataataac taataatata gatgatatat ttaataaaga tatagatata cttgttgaag
    18541 tcatgggtgg tttagaacca tcattatctt atataaaaaa agcattaaac aataaaattc
    18601 atgttgttac agcaaataaa gatttgttag ctgaatgtgg agatgagctt gcaaaattag
    18661 caagtgaaaa taaagtaagt attaaatttg aagcttcagt tgcaggaggt ataccagtat
    18721 taaaaccaat aatagaatca ctagaaggaa ataatataga tagtataaat gcaattttaa
    18781 atggaactac taattttata ttatctaaaa tgtatgatga gaatctatct tatgatatgg
    18841 cattaagaca agctcaagaa ttaggttttg ctgaagcaaa tccagagtca gatgtattag
    18901 ggtatgatgc tgctagaaaa ctatcaatat tatcaactct agcttatgac aatagagtat
    18961 attggaagga tgtgtattta gaaggaatta cagatataga tgaaaaagat attgaatatg
    19021 caaaaaaatt aaattgtaaa ataaaattga ttggtcaaag taagtatgaa aatgataaag
    19081 taagtgcttt tgttagacct gttttagtag aaaaagataa tatccttgct agaatagaca
    19141 atgagttcaa tgctgtcata gtgaatggag attcagtagg agaagtttct tttgtaggta
    19201 aaggcgctgg aagtttagca acaggaagtg cggtttattc agatgttata gatataatag
    19261 ataatagagt ttcaagtata gattctttta ctaaagataa aatacaagtt aataaaatag
    19321 ttagagaaaa gtgtggagca cttcttagat ttaaaaaatg taataaagat gaaattctaa
    19381 atatagttga gaattgttta gtaaaattcg atattttaaa tgatgatgat gaacttgcta
    19441 ttatggttta tgctgattct gaatatgaaa taaacaacag tttatgtcta ataaaagata
    19501 aaggatactg cgaaaaaatg aataaaatgt tgaaaataag ttaaaaaaga aaaaatataa
    19561 gtttatatgt aacatatttt gacgatttta atactatata gtatgagatg aaacaaaaca
    19621 tctcataaaa aaattaaaat tcaagggaga gatatgttat atggaaaata aaaaatatgc
    19681 aaatggtggt tattcagaag actggtatga aagaggagaa tctacagcta aatggttcca
    19741 gaatgataga gaagaatatg aaagagcagc atacgatgaa gatagagaaa gaagaggctc
    19801 aaactgtgga tgttcagatt caggagaaaa tagacctaga aactgtgaaa gatttagaag
    19861 agaagcagag ataagagaaa gagaagcaag agaagcattc tgtgaatctt cagagagaaa
    19921 gaaagaagca ctagcatatg aatgtgaagc tagaaaatta tgggaagaag cagaaaaata
    19981 ctgggatgaa tattcaaaat acaactataa aggaatcgaa tatttagcag aagctgctag
    20041 attatttgat gaaggtatgg aatgtgaagc tagaagaaat ggaaataatg gaggaaacaa
    20101 taataattgt tgccataaat gccataaatg taattgtaac tgctgtagaa aataaaaaaa
    20161 ttaattgaca aatcaatagt taaaatatat catattatgt ataataaaga gaaaattcag
    20221 aaaaaaataa tgattagaag aaatataagt atatttatta gacaaatagt gctcagttag
    20281 tgaggtgagt acaatatgat taaactccca tcctctagct tttgatagag ggtgggagtt
    20341 tttatttatt tatatatctt aaatgaggat aatattgtaa aataaatgag tatgagattg
    20401 taaaattagg aggtaaaaaa tgaagagtat taaaacatca agtatgaaag aaaaggtaga
    20461 tgaaaagata ttaagagagc aagtaagttc tataatagaa gatataagaa ataataagga
    20521 tgttgcactt aaaaaatata atgagaagtt tgatagaaat acaagagatg aatttagaat
    20581 tactaaggaa gaaataaagg aagcttataa gcatgtagat gacgaattta taaataactt
    20641 aaagattgct gccaaaaata taagagaatt tgctaaagct caaaaactaa gttttgaaaa
    20701 cccttttgaa aaagaaattt atccaggtgt aatcttaggt caaactaata ttccaataga
    20761 gtcatgtctt gcatatgttc caggaggtca gtatccattg ttttcaacag ctcttatgct
    20821 tataatacct gcaaaagtag caggagtaaa aagaatagtg gcatgttcac caactatgaa
    20881 aaatactgaa aagataaatc ctaagacatt ggtagctatg gatatagcag gtgctgatga
    20941 aatatatgca actggaggag ttcaagctat agcagcattt acatatggaa cagaaaagat
    21001 taatccagtt gacataattg taggaccagg aaataagttt gtaactgaag ctaaaagaca
    21061 atgttatgga caagtaggga ttgattttgt cgctgggcca agtgaagttc ttataatagc
    21121 ggatgaaact tcaaatccag tttatattgc agctgattta ttagcacaat gtgagcatga
    21181 tttaaatgca agaggaatat tattgacaaa tagcttggaa gtagcacaaa aagttgaaaa
    21241 aaatatagaa agtatgctaa aagatttgcc aacaaaggac atagcatata gttcatggga
    21301 aaataatgga gagataatat tggttgatga tatggaagaa gccattaaaa taagtaattt
    21361 ttatgcacca gaacatttag aagttgcagt aaatgagtat aatgatattt gtgatagatt
    21421 aactaactat ggttctttat ttattggaaa tctttctgct gaggtgtttg gtgactatgt
    21481 ttcaggtacc aatcatacat taccaacact taaagcatca agatatactg gtggtgtttg
    21541 ggttggaact tttatcaaaa cttgtacaaa gcagatattt aatgaaaaag ctatacaatc
    21601 attagcacct gtagctgaaa agctggcaaa agaagaggga ttatatgcac atgcaaaggc
    21661 tgctgaggtt aggtttaaaa agtagtaaaa aattttattt tggcagtcga gatttagtgt
    21721 tagactgaga taaggaaaac gctgagaacc cgaataaata ctatattttg tgggcctcgg
    21781 cgtttctttt atatctgtat ataataggat aatactacct gtgtgtttaa atgctagctt
    21841 aatatttctt ttctaaataa tgtttatgaa atagttaata tttttagtta atacttgaaa
    21901 aaaacttttg ctaactgtgc aaattcatct gcattcgtca atatagcagg gtgtcctccc
    21961 tgactaaaaa tgtgaatact tgcttttgaa ataaaagttg ccatttgttt gtactcttct
    22021 tcaagattat gacgacacat ttcgtcttcc ttacttcctg taaataaaat tggcatttct
    22081 aatttacaca attctttatg aaacaaaggg cgtttctcgt ttgcacattg caatagagct
    22141 tttgtatcaa gattaacaac attctcccaa tcttttccat gacaccattc atagaatttt
    22201 cgtgcttgaa tatcttgctt tgatttttcc cttccagtga ttagattatc tatgaagtta
    22261 tcattaagag tacgtccatc aaagctatct gctataattg cttcaactaa atcgggacgt
    22321 tctaatccag tattaacagc tgcccatgct cctccacttg ttccaattaa gccaacttta
    22381 ccacaattca aatgttcaat caatgctatt gtctgtaagg cttcatcatg ccatatgtca
    22441 ggagaaaatt gagaaactct atccgactgt ccattaccta aaaaatcaat taagatacat
    22501 ctaaaatttt ctacatataa tggcacaagt gctttaaaca tattagaaga accagtatta
    22561 ccatgcagga aaattatcgg tttaccttgt ccatattctt tataaaaaca agaattacct
    22621 tgatactgaa aatatgacat atattttcct cctttttatt tgttaagtta attataacaa
    22681 tataggtatg aatgaaaaac ctcatgttct ttcttagaat aaaaatgtgc cacgcataaa
    22741 acgtgacaca tttctaatta taaattttgc tgtttaagta ttctttgtat gcttttctct
    22801 gtcaaaaagt atttctctga taactgcttg atactcattc cattttggaa atttatacag
    22861 atttgagaat tacgagaagc taagacttgt ttggtgtttg tgttttcacc ccaatgcttt
    22921 ttgtgctctt gctttttggg aatataaatc actttaccat ctatatattc ttgcacttgc
    22981 tcaatcagtt ctttaggcag taagtcaatc gaattttgat agctcatttt gcacctccta
    23041 aataattatt ctttaggatt agcaaaggct actgccactc acaattaaaa ataaaatttt
    23101 ttattatgta caatagcctt tgctatgcac aagttccaat tcccataaac taaatactcc
    23161 tttctggttt gaaaaattca tctgaacatt gatttcgctt ttaagataga gttcaatgac
    23221 aaatttacat ttttattata tatattcaga tagataattg caactgtctt tgtatgagga
    23281 tattttatta tagtcaatac tataatatgt atttatatca tcaaactaat atgaacagtt
    23341 gaaaggaata tgaacaataa aacttagtca ggaagaataa ttatgaagca gacaatagag
    23401 aaatttgatt ttaaagtttt attgaaaatt tattgacaaa tttaacttgt aaatattata
    23461 ctaaacttaa taaataaagt ttataaagta aggtgttgtc tatgaaaaat atagttggaa
    23521 aaccgcaaac gataaaaaaa gtaaatgaag atttaataaa aaatattata aaaaatgaag
    23581 gtcctataac aaaaccagaa atttcaaaca ttactgaatt gagtttagct acagttaata
    23641 agattgtaga gcaattagct ttgaaaaatg aagttaaagt aagtggacta agtgaatcta
    23701 ctggaggaag aagagctcaa ttatttgaaa taaatgcaaa cttagaacac ataatagctt
    23761 tatattatta tagaaattgt tgtatagggg tagttgccaa tttattaggt gaaataattt
    23821 atcaagaaga atttaatata agaatggata catatgaaaa cgttaaccaa gatacttttt
    23881 ttgttataga tactctaata aaaaaggaaa aaggaaacaa tataagggct attggaattg
    23941 gtgtcccagg agttgtaaaa aaaggtgtag ttaaaaatat accaactata aaaagttggg
    24001 aaggtataaa tttagaaaat ttaataaaga gtaaatacaa tactaaagtg tttatagaaa
    24061 atgatacaaa tattactact attgggattt atcaaaaaca gtataaagac aaatataaaa
    24121 atatggctct tatgtattta gaagatggta ttggttcagg tatagttata aatggtgaat
    24181 tgtatttagg ttctacaaat tttgctggag agttaagtta tctaaatata gaatttggta
    24241 agtataactc taacaactta aatcttgaag attatattat gagtttaaga aaaagatatt
    24301 tagaaagtaa agataataaa tttaagaaag aaattttatc tataatatca aactttataa
    24361 ggactcttgt atgtgtccta aatccacaag ctatgattat acagtgtagt atattaacca
    24421 agagtgacat ggagtttata gaaaaagatg taagcaataa tgttggagaa gaaaattttc
    24481 ctaaattaat aaaagtagag tcattgagag aaggaagtat aaatggagta attagtatgt
    24541 gtttaaaaga aactgactat caatattcct tatctaataa aaaaggagga tattaatgtg
    24601 aaatatttgg tttcaataga tggaggggga acaaaaacta aattttgtgt aagtgactta
    24661 gatggaaaca tacttaaaga acataccact ggttcaacta attataagtc agttggaatt
    24721 aaaaaaacat atgaaaatat taataatgga tttaaaaaaa tattaaaaga tttatatatc
    24781 gattatgacg atattgagta cactgtattt ggtatatctg gttgtgattc tcctaatgat
    24841 tataagatta taatggatga aatattaaaa attgggatta acaaagaaaa aatatatctg
    24901 gcaaatgatg cagttttagc tttttatgca caagcggatt cccctggttt agtaattgtg
    24961 gctgggacag gttcaattat actgggaatt aaagaagatg gagaaatata tagagttggt
    25021 ggatgggggt ataattttag tgatttgggt tctggatatg atatagggag aaaattatta
    25081 aagaaagtat tgctttattg tgatgaatgt catgagtata gtgatttatt tagttgtgtc
    25141 ttggatttct ttggagcaaa tagttttgaa caacttgcat atatgattac tgatataaac
    25201 aataatgttg aaatagcaaa cttagcttca ttagtgattg attgtgccga acaaggtgat
    25261 aaattggcta ttgaaatatt aagagaatca tcaactgaac tatctaaatt agctcaagta
    25321 atattgagaa aaatatctaa tccaatgaaa gtggataaaa gcgaaataaa tatagttttg
    25381 tctggtggta cattaaataa aactgtatat gctcaaatgt taatgaataa tttaaaaaca
    25441 gaaaatacag ataaaaattt aaaatttata tctcaagaaa atcagccagt gtatgggggg
    25501 ataagacttg ctatggcatt agctaatagg agggtcaagc atggatagaa aaaaggtaat
    25561 agtacaaatt tttactggag gttttaaggc taaagaaata aagtttaatg atataaaatg
    25621 taaactacta gaattattgg actgtattga tatagaaaag gttatcatgg gatggagtgt
    25681 agatgaagaa ttatatgtag aaactaaaaa actattaaat aaatataatg ttgaactata
    25741 tctttggttg ccatgttttt ctgaaatagg attacttgaa aaaagtaatt tgctaattga
    25801 ttatatgggg aaggaagtca agggctatgc tttacaagag gatgaaaatt ttgagtttta
    25861 ttgcccaaat aataaggaaa atataaaaaa tataaagaaa atatatagta cttattttag
    25921 tgatattaaa tttgatggaa tttttttaga taaaataagg tatggttctt tttcgaataa
    25981 actaaatggt gtttttaatt gtttttgtaa agattgttta aatatatatg aaaaaaataa
    26041 tattgataaa gagtcgttaa tgagagaaat gaataatgtg tctaagggag ttatagataa
    26101 tgaagatact ccatttggta taacagaata cattaatgga aaatacaaat ttaaaaataa
    26161 aatatgggaa gatttttttg aaacaaaaaa tcaaaatata tacaaggcat tattagagat
    26221 aagtaattat tttaaaagta agggattgaa aataggtata gatacttttt ctccttttat
    26281 ttcttacttt acaggtcaag atttagaaaa attacaagaa gtagcagatt ttataaaacc
    26341 aatgatgtat agaactacaa aagctccagc tggtttacca tttgaatttg aaaggtttat
    26401 tgatgaatcc atacataaga ataaagagat tggaaaaaga aatttcttag atattttagg
    26461 aattaaggag tatgaaggag atgtatatcc tatagaattt gtactagagg aaatcgattt
    26521 tatgacaaga aatcttaata aagatatata tgtaggagtt gaaataaata aaaaggaaat
    26581 tatatctcct gttacaccta agtacataag tgaaaatatt tcaaatataa ataagacaac
    26641 agtaaaagga tatgttttat cttgggattt gttgtcagca ccaaaagaaa atattgatga
    26701 aattataaaa tggtttcgga attagtgagg tgataaagtg agtaagatat tggaaatgca
    26761 acaaatatat aaaagttttg gaaaggtaga tgttttaaaa gggataaatt ttgagttaga
    26821 aaagggagaa gtaagagctc ttttgggagc taatggagca ggaaaatcta cacttattaa
    26881 aattttagga ggagttcatt ctcaaactaa aggtaaagta attttgactg gtaatgaaat
    26941 aatgtttaaa aatccacatc attctaaaga aaatggtatt agtataattt atcaagaatt
    27001 aagcttgata ccaacgctta cagtacttga aaatattttt cttggtagag aaattttaaa
    27061 aggaagtgtg tttttaaaca agaaagacat gcttgaggaa tgtatgaaaa tttgtgagga
    27121 ttttgatttt aatatagacc cacatatggt agtttctaat ttaagtgtag caaaccaaca
    27181 aatggttgag ataatgaaag ctgtttcatg taattctgaa attatcataa tggatgaacc
    27241 tacaacatca cttactaata atgaaaaaga tgggctattt aaaataataa gtagactaaa
    27301 gtctaaaggt aaaacaatag tctatatttc tcacatatta gaggaagttt ttaaagtatg
    27361 tgatagtgca agtataatga aaaatggaat tatgattgga acttatgatg tagaggattt
    27421 aactaaatta aagataactc aacttatgac aggaaatgat aatcacaaac ttattgagaa
    27481 gaaggattat acatttgcaa aatatgaaga gccaccagta ctagaagtaa agaatataag
    27541 ctggtctaac aaaataaaaa atgtttcatt taaagtttat agaggcgaag ttgtaggtct
    27601 tgcaggtctt gtaggttcaa gaagaactga aattataaat ttgatatttg gtatagataa
    27661 taaagatagt ggagacattt ttatcaatgg taaaaaagta gacataactt ctcctaaggt
    27721 tgctataaaa aacaaaatag gattgattcc tgaagataga aaaaatctag gattaatttt
    27781 agaacaagct atatacacaa actcatcatc agtacaaatt gataaattca agaaattctt
    27841 gtttatagat agaaataaag aaattgagtt ttctaaaaaa ggtgttaagg aattaggaat
    27901 aaaagttaat gatgtaaagc aagaagtaaa agagcttagt ggtggaaatc aacaaaaagt
    27961 agtagtttct aagtggttag agaaagattt agatttaata atatatgatg agccgacaaa
    28021 gggaatagat attgctgcaa aagaagatat tttcaataca atcaaagaat tttcaagtaa
    28081 aggaatagga gtaatattta tatcttctga tttagaagaa gtaataagag taagtgatag
    28141 aattttagta actagagaag gttctattgt agatgattta aaaaatgaga atatacaggt
    28201 tcaagatata atgaacagta tatttaatgt ataaaaaatg aaaatggagg actgaaatga
    28261 agaaagcttt tgagaagtta aatttaagag attatggagt attattaggt tttttagctt
    28321 tatgtatagc tataagtatt gcaactccat catttttagg taaacaaaat atacttaatc
    28381 ttttacgaca atcttctata attggaataa tttcagcagg tatgactttt gttataatat
    28441 ctggaaactt tgatatatca gttggagcag tagcagctct tgcaggtgct ataacaatga
    28501 aatttgcgac tggtggaact aatttgtttg catccatgct cttaggtgta gcgatttgtg
    28561 cagtaatagg attaatcaat ggtatagtag ttgcaaagat aaatgtacca tcattaatag
    28621 ccactatggc tatggtcact attgtaagag gattgctgtt gatgcttaca ggaggatatc
    28681 caattactga aaatatacca atacttgatt acattggaaa tggatatttg tttaatatac
    28741 caataccagt agtaattttc tttatagtgg tgttagcttc atttattgta cttaataaaa
    28801 ctaagtttgg tagatatgtt tattctgtag gagggaatca agaagcaagt aaacttaatg
    28861 gtataaatgt agattctcac aaagtgaaag tatttattat taatgctgta cttgcaggaa
    28921 tagctggaat cgtactaaca ggaagacttg ggactgcaac tgcgattgca ggtgaaggat
    28981 atgatatgga tgcaattgca tctgtagtaa taggaggtac atcagtggca ggaggttctg
    29041 gttctgtact taaaactgtt ataggagtat tacttatgag tgttataaac aacagtttta
    29101 atcttcttgg aatagatgta tatttccagt acatatttaa aggattaata attttagcag
    29161 cagttggatt tgattcttat agcaagaaaa aacttgcttc aagataaaaa ataaggggga
    29221 tttttttatg aaaaaattaa tgaaacattt agcattgttg ctatcacttg ttatgatatt
    29281 tggtttagtt ggttgttcaa atggtggtga caaagaaaaa aatggagaaa agaaaatagc
    29341 agtattacta ccaggttcaa caggttactt tgtagcaact aaacaaggta tagatgcaaa
    29401 atctaaagaa ttaggtgtaa gcgtggaata tgcagatgct caatgggatg ctagtaaaca
    29461 attatcacaa gctgaagatt ttatggctaa aggggtagac atgattgcat tatgtggagt
    29521 tgattctgct gtaagtgaga gaatagttaa agctgctaat gattcagacg ttcctatagt
    29581 agcatttaca aattctatag gaagtaaccc aactggagaa tttaaaggtt tagttacata
    29641 tataggacaa aatgaggaag aaacaggagc attaactgga aaaattgcta aaaacttatt
    29701 aggagaaact ggtgggaaag ctgtattaat agaaggagta ccaggaacta ctccacaagt
    29761 attaagaaaa aaaggtcttg aaaaagaatt aaaagatagt aatatagaaa tagtttataa
    29821 tcaaacttca aattgggaga aagagcaagc tttaaaggta acagaagatt taattcaaaa
    29881 gaaaactgat tttaatgtta taatatgtca agatgataat tctgctacag gtgcaggtca
    29941 agctttaaaa gatgctggtc ttaaggataa agttaaagtc ataggtttgg gtggaagtaa
    30001 agatggatta aaagctataa ctgatgggtt aatagatggt acaacttata tgtctgctgt
    30061 agaagaaggt ggattagtca tagaaaaggc atctaaattc cttaaaggtg aaaaaataga
    30121 acctgttaca caaattaaac aagttgaagt taacaaagat aatatttcag agttcaaggg
    30181 tgaatggtaa attgtcatag ataataaata agatttttag agttacttta gaaactatat
    30241 aaagtaactc tttttttaat ttaatttagt gataaattat tgaatagtat ttctaaacaa
    30301 ataaaaaatt aatataaaca aagacttaat tatactaaat tataagtaaa actaatataa
    30361 acttatggaa aaaaggaaaa cgtctaaagt attggaatta ctatagtgta ggtgtggtat
    30421 taaaaaaaca taaaaaaaca taaaaaaatt gttgaaaaaa atatagaaat tttgtataat
    30481 taaagatttg aaaagtgcgg tatacaaagc caattctttt gtatataata tatataaatt
    30541 cagaaaaagg aagggatatg tatggccagg cggttatttt tagaaaggat tggacttata
    30601 tttttaggtt cagcaatatt agcatttgga gtttataatt tttattattt aaacaacata
    30661 acagaaggtg gagtgttagg tattttacta ctgttaaaaa atttattcaa tatacaacca
    30721 gcaatagcaa atgttgtaat agatggtttg ctacttttag taggatataa atttttcggt
    30781 aaaaaattcc ttatatattc aatagtagct tctataactt tttcagtgtt atatgactta
    30841 tttgaagcta taggacctct agtaccacaa tcacaaaata tgcttttatc aactattttg
    30901 gcaggtgtaa ctgttggaac tggtgtaggt atagtagtaa aagcaggttg tgcatcagga
    30961 ggagacgatg ctttagcatt agtaatttct aagactacat ctttgaacat agggcaagtt
    31021 tatttagcaa cagatgttat tgtattgttg ttatcattat tttatctatc agcgtttgat
    31081 atcttttatt ctttgattgc tgttacaatt agtggcaagg taattgattt tatttattat
    31141 catggtaaaa gtttggatat ggatataagt aatgatatag ttccagaatg ttaatataaa
    31201 ttaaataact ttagatttta ttaggggtgg cagatttgcc accttttttg tgtctaaatc
    31261 tttatagaaa gtataatcct atctaggttt ttgcaaatct caaatataga attatgttga
    31321 aatgactggg aatacgattg ctagatgtgc attttaaaaa cccaatttta tatttatgaa
    31381 atactaatca ttaatggatg tttcttaatc ctatctttat acctaaaatg ataagataaa
    31441 tgaagtagca atacaaaact aaaatttgta aaagagatta aattaaatgg aggagctaaa
    31501 atgttggata tagttatgat agcattgtta ttcataggat ttgtttccat gaagttactt
    31561 gctgattggt gtgggaaaca aactgaaaaa aaataatgta atgtatggag ggattaccta
    31621 tgtggtttct ggcagttgta attataatgt taataatata tttggtatat gcgcttatta
    31681 atcctgaaaa attttaaaca gtataaggct aataagtaag ttgaattggg gggaacaatt
    31741 tttatggtgc agatagtaat agtgttagcg atttttatga ttttagtaat acctatgggc
    31801 aagtatttat atcatatagc tactaatcaa aaaacatttg gagatagact atttgataaa
    31861 gtggacaatt ttatatataa ggtatgtagt atagataaga aaaaagaaat gaactggaaa
    31921 cagtatgcat tagctttatt atttacaaat gcagttatgg tatttatagg atatatcata
    31981 cttagaactc aaagtatgca tatttttaat ccaagtggaa ttaaaagtat ggaacaagga
    32041 ctatcattta atacaataat aagttttatg acaaatacaa atcttcaaca ttattcaggt
    32101 gaatcaggac tttcatactt tagtcagatg acagtgataa tatatatgat gtttacatca
    32161 gctgcaacag gatatgcggc tgctatggca tttgtaagag gtctagtcgg taaaaagaaa
    32221 acattgggaa atttttatgt agatttaatt aggataacaa caagagtact tttaccagga
    32281 gctttaatta ttgggcttat attagctaca caaggagtac cacaaacttt tgctgggaca
    32341 gaaacagtga ctactataga aggaaagctt caagatatag ctagaggtcc tgtggcagct
    32401 cttgaatcaa taaaacattt aggaacaaat ggtggtggat tctttggttc aaattcatca
    32461 cacccatttg aaaatccaac aataatatca aatatagtag aaatattatc tatgatgata
    32521 ttaccaggag cttgtgttgt tgcatttggt cacatgataa agaataaaaa acaaggttgg
    32581 gttgtgtttg gcgcaatgtc aatcatattt ttaataggtc tagtagtgtg ctttaaagca
    32641 gaaagtgcag gaaatcctat tttatcacaa ttagggctta atcaaagtat gggaagtatg
    32701 gaaggtaagg aagttagatt tggtatagca caatcatcat tattcacaac agtgactact
    32761 tcctttacga caggtacagt taataatatg catgatacac tgactccttt aggaggatta
    32821 gtaccacttt taaatatgat gctaaatgta gtatttggtg gtaaaggcgt aggtctgatg
    32881 aatatgttga tgtacgctat aatagcagta ttcttatgtg gattaatggt aggaagaaca
    32941 ccagagtttt taactaagaa aatcgaagga aaagaaatga agcttattgc tttacttatt
    33001 atattacatc cacttttaat actaatgttc tctggtcttt cagtagcaat accagcagga
    33061 cttgaaggaa tttcaaatcc tggattccat ggattatcac aggtattgta tgagtttgca
    33121 tcttcagcag ccaataatgg ttcaggattt gaaggtctag gagataacac tatgttttgg
    33181 aatataacaa caggtatagt tatgttcttt ggaagatatg tgtctattat agtactacta
    33241 gcaatatcaa gtttgttagc atctaaaaaa gctgtaaatg aaagtatagg aacacttaga
    33301 acggataatt ttacatttac aatagtttta gtacttgttg ttttaattgt tggtgcttta
    33361 acatttttcc cagccttagc gcttggacct atttcggagc atctagtgtt gtggcactaa
    33421 aaaattggga gggagtaaag ttttatggaa actaaaaaaa caaagtttat tacaaaagat
    33481 attttcaaaa cttccattat agaagctttt aaaaaattaa atcctaaata tatgatgaaa
    33541 aatccagtta tgtttgttgt agaaattgga tttatagtaa cattgatact tacaatagta
    33601 cctagtttgt ttggtgatga aggcaataac ttaagagctt ataatgggat tgtaacaata
    33661 atcttattta taacagtcct ttttgcaaat tttgctgaag ctgtagctga aggaagaggt
    33721 aaagctcaag cagatacact taagaaaact agaaaagata cagtagcaag ggttatagat
    33781 acgttaggaa atgaaaaaat gataagtgca agtgagctaa aacttggtga tattgtttta
    33841 gtaaatgctg gagaagttat cccaaatgat ggagaagtta tagagggtat agcttctgtg
    33901 gatgagtcag caataactgg agaatctgca cctgtcatga gagaatcagg tggagatttc
    33961 gcatctgtta caggtggtac tacagttgta agtgactggt taaagataag aataacaaca
    34021 aaaccaggag aatcgttctt agataaaatg atagctcttg tagaaggtgc ttcaagacaa
    34081 aaaacgccaa atgagattgc tttaaatact ttgttagtag ggttaacact tatatttatg
    34141 gttgttttaa tatctcttta tccaatggca aactatgcag gtgtaaagat tccaatttct
    34201 actatgatag ctctcttggt atgtttaata ccaactacta taggtggatt gttgtcagct
    34261 attggtattg caggtatgga tagagtaact agatttaatg taattgcaat gtcaggaaaa
    34321 gcagtagaag cttgtggaga cgttgatact atgattttgg ataagacagg tacgattact
    34381 tatgggaata gacttgcatc agaatttata gcagttggaa atgcagattc aaaagattta
    34441 ataaattatt ctgttatgtg ttcattaaaa gatgatacac cagaaggaaa atctatagtt
    34501 gatttaggta tgaaattagg ttcaacaggt aagacaaaag aggcagagga agctgagttt
    34561 gtagaatttt ctgcacaaac tagaatgagt ggtattaact tagctgatgg tagagctata
    34621 agaaaaggtg cttatgatag cataataaaa agagtaaaag aatctggtgg tacagtgcca
    34681 gaagatttag aagaaaatgt aaatcgagta gctaaactag gagggacacc tttagtagtg
    34741 tgtgtagata atgagatata tggaattatc tacttaaaag atacagtaaa atcagggctt
    34801 gtagaaagat ttgctagact tcgagagata ggaataaaga caataatgtg tacaggggat
    34861 aatcccttga cagcagctac aattgcaaga gaagctggag tagatggatt tattgcagaa
    34921 tgtaaaccag aagataaaat agatgcaata aaaaaagaac aattagaggg taaaatagtt
    34981 gcaatgacag gtgatggaac aaatgatgct cctgcacttg ctcaggcaga tgtaggtctt
    35041 gcaatgaata gtggtacaac atctgcaaaa gaagcagcaa atatggtgga tttagattca
    35101 gacccaacta agatattgga agttgttgaa ataggaaaac agcttttaat aactcgtggg
    35161 gctttgacaa catttagtat agcaaatgat gttgcaaaat attttgcgat tataccagct
    35221 atatttactc ttgcaatacc tcaaatggag atattaaatg taatgaaact ttctacgtca
    35281 tttagtgcga tattatcagc tttaatattt aatgctataa ttatcccatg tttaatacct
    35341 cttgcaatga agggagtaaa atatagacct atgaaatcac aagctatgtt aatgagaaat
    35401 atgcttatat ttggtcttgg tggagttata gtaccatttt taggaataaa attaattgat
    35461 ttattgatta cacctttagt aatggttctt aatttaggtt aataaatgga ggatataaaa
    35521 atgaaaacat taaagccagc attacttgta agtattgtat tgttagttgt ttgtggactt
    35581 gtatatccac ttgttttgac aggtgtaagc caagttgcat ttaaagataa agctaatgga
    35641 agtatgatag aagtaaatgg tgtaaaagta ggttcagaac ttattggtca aagctttaca
    35701 gatgcgagat tctttaaggg aagagtatca tcagtaaact ataatactta tacaaaagaa
    35761 gatttagtac cagataaaga tggaaacaca tcttatggag gtgtatcatc agggtctttt
    35821 aattatggag ctacaaatcc agaacttcat gatagagtac aaaaagatat agaacagttt
    35881 ttaaaggata atccaactgt caaaagagag gatataccaa cagatttact tacagcatca
    35941 ggttcaggac ttgaccctaa tataagtcca gcctctgcaa aaattcagat accagctata
    36001 gcaaaagcgt ctggaatatc agaaagtaaa ttacaaaaaa ttgtagaaga taatacttcc
    36061 aaaaaacttt ttggagtttt aggtgaagat agagttaatg tattaaaggt taatgtagag
    36121 gtcgcaaaaa tattaggtct tatatagtat tttgtggaat tttagcaaca aaatattact
    36181 caagatttaa tttaaaggtt aaattttaaa gagtgaaaat cgataatgat tttcattttt
    36241 ttatttgaat attactaata tttggaataa agtgattgta agtataaaat ccaaaaaaag
    36301 gttgcattat caaaatgata atactataat atatttatcg ttatcaattt gataatagat
    36361 ttggacatat aggaggaaat agtatgttat ataataacgc attagagttg ataggaaaca
    36421 cacctgtagt aaggctgaat aatttaggct gtactaatgg gtgttctaac atatatgtaa
    36481 aattagaaaa agtaaatcca gcaggaagta taaaagatag agctgtttat gaaatgttag
    36541 agggtttaga gcaaagagga gaattaaaaa agggagatgt actagttgaa gcaactagtg
    36601 gaaatactgg tattgcatta tctatgatag gaaaattaaa aggatatgaa gtagtaatcg
    36661 taatgcccga aacaatgagc gttgagagaa gagatttaat gaaggcttat ggagcaaatt
    36721 tggtgcttac agatggtaaa gctggaatgg caggttccat agaaaaagca aatgaacttt
    36781 taaaagaaaa tccaaactat aaaagcttaa aacaatttga aaataaagat aatccaaatg
    36841 cacattataa aacaacagca gttgaaatta tgaatgatgt caaagattta gatatattcg
    36901 tatgtggagt tggaacaggt ggtactttaa taggaactgc aagatattta aaagaacaaa
    36961 atccaaatat aagagtaatt gcacttgaac caaaaaaatc tccagctata tcagaaaaca
    37021 agactggacc tcataaaata caaggaatag gtgctaactt tgttcctgaa aattatgatt
    37081 ctagtgtagt tgatgaagta atattagtag atgatgaaga tgcatttgat acagttaaat
    37141 tacttgcagc aaaagaggga attttagtag ggatatcttc tggagcaaat atatttggtg
    37201 ctatagagat ggcaaaaaag tatccagata aaaagatagt tacagtagct ccagatggag
    37261 ttgataagta tatgtctatg ggaatatttt aataatttta tttgagggag tgaatacttt
    37321 gtttaagaaa ataaataaag atattgaata tataatgaag aatgatccag cagctaggtc
    37381 taagatagaa gtttttttac tttatccatc agttcatgca atgataatgc ataggatggc
    37441 tcatgctctt tataagaaga agaagctttt tacagcaaga ttaatatccc agatatccag
    37501 atttatgact ggcatagaaa tacatcctgg tgctaaaatg ggggaaggta tattgataga
    37561 ccatggaatg ggtgttgtaa ttggagagac agctgaagta ggtaataggg tgacaatata
    37621 tcaaggagct actttaggag ctacaggaaa agatactgga aagagacatc caactgttgg
    37681 tgatgatgta ttaattggtg ctggaacaaa aatactagga cctttaaata taggttcaaa
    37741 ttcaaaaatt ggtgcaaatt ctgttgtcgt taaggatgta cctaatggtg caacagttgt
    37801 tggtatacca gcaaaaatag taaaaataag aaacctagaa cctgtaaaaa agaataaaaa
    37861 agaagtgtcc tatgagtatg atgaattaga taatgtatat tatatatagg atttatataa
    37921 ctataaaata aactttataa aaattatttt aaaaggtatt tttttcaaat ttgaggacat
    37981 aataaaaaag aactgaatgt tacagttttg gttcatcaat ctttgataaa caagatttta
    38041 aatttgtagc gtctaggagg attttattat gaaagcaaat gttaatcaag atacttgtat
    38101 aggttgtgga ttatgtccat caatatgtcc agaagtattt gatatgaagg atgatgggaa
    38161 atcacatgtt atagttgatg aagtaccaag tgatgctgaa gaatcagcag cagaagctag
    38221 agaaagctgt ccagttgatg caatagatgt gcattaataa aataaatgcc tataaaaatt
    38281 gaattttcaa tttttatagg catttgtctt ttattcagtt agtatttcaa cacctgtttc
    38341 tgtaactaaa accatacatt cccattgagc agataattta ccatcttttg tataaatagt
    38401 ccaattattt tcttcatcaa caaaaatttc gtgagttcct tcatttacca taggttctat
    38461 tgtgaatatc atcccaggaa ccaatagcat ctcagtccct tttttagata cataacttac
    38521 aaaagggtct tcatgaaagc ttaatccaac tccatgacca cctatttctc taacaacaga
    38581 gtatccattt ttctttgcat aagagttgat aacatcacct atatctccta agaatcccca
    38641 tggtttggca acttcaagac ctttttggac acattccttt gtaacttgaa ctaatttttt
    38701 tgcattttcg ttaacattac ctatcataaa cattcttgaa gcatctgaat agtatccgtt
    38761 aaaaatagta gaaacatcta catttataat atctccatct tttaagataa tgtctttact
    38821 tggaatacca tgacaaactt cattatttac tgatgtacat acacttttag gaaatccatt
    38881 atagtctagt ggagcaggga tagctccatt ttgtagtgta aagtcatgaa ccaacttatt
    38941 tatatcttct gtagacattc caatttcgat tttatctgaa atataatcca aaatttttgt
    39001 gtttattaca gcactttttt ttatttcctc aatttgactt tcatttttta taatatcatg
    39061 agttggtaca atatgaccta acttagcaaa tgaatctatt ttattatcca tatccatatg
    39121 acatttttta tattttttag tacttccaca ccaacagcta tcatttctat ttaacttcat
    39181 aacttttcat ccttctttta ttatttattt aaattatggg ttacataaac tattttaaaa
    39241 cataaccatt ataaatatta taacataaat actaagaaat cctttataga tagtgattta
    39301 ttcaaagtat aaattattat atggttattg ttgatgaaaa gaggatagtt aaatgtatta
    39361 ttggatatta gcgcacttaa tagtttagtt caagtttttc aagaaaatca tatgttagag
    39421 gacaattttc ctttaaagta ttttttgaat cttcatcaag ataataatgt acaaatgatt
    39481 ctgcaaaaaa ttcttctata aacttgctta tatatgcatg tgcaacttgc ttagggtcat
    39541 aaagcttata tctttcctga gcaaatactt ccataaattc ttcagaattt gattttttat
    39601 ttaaaacatt tttatctata gcatgtgcag tttcatgtaa ttctaaattt tttgatgaat
    39661 gtacatttgc atagctaggg ttactataac ctattttagc cactatagaa ttatttctat
    39721 atataccagg tacatctttc caagtcttgc ctgatcttac ccaacatgga ggtaattgat
    39781 attttaaatc actaaattct ggttcatcag ttagatttga atttattaat tttattttta
    39841 tatccttttc tttaagtgtc tgtaaatatt taatatttat ttttgcaagt ctgtttttca
    39901 tattttcaac ttcatcttta tcataattca agtcatcagg aagtataagc atttgttcaa
    39961 ttatatctat actactgtct tttatatttt cagattcttc atcaaattta tattgaagag
    40021 tattattttc atagatagtt atatttgtat tattttctat agaataagag tgaatatcct
    40081 ctaaatctaa aatattcatt tgttgtaaaa tatgttttgt ttcgccactc cacactacta
    40141 aagaaagtga attaaaatta attagtggat tttcttttgc ttttatatca ttgcttatat
    40201 ctacatcaaa tacatattca attgagtctt ctatgttaag actatctgta ttctttttaa
    40261 tatataaata cgcttgtttt aagtctatat aaatctcatc taaagattcg tcataaaatt
    40321 ctgataaatt attagtaaag agttcttcac ttaaaagttc atttttatca gttatacttt
    40381 caattaaaat attaatttct tttatcatat tgtaaatact tatttttcta tttgcttttt
    40441 cactagatac attgcatggc gcaagagcaa tgcaataaaa atctttttct tttttataaa
    40501 aggctttaag tgattttgat aaaagtgtaa agtctacacc tatctgtaag ttgaccttaa
    40561 gtgagtcatt ttgtaataat tcagtattat ttcttttagc tgtatttaaa ttgttttcca
    40621 taatcattgc ccccttttta ttagggtatc ataatttttt tatatgtgtc aatttgtttt
    40681 tttgaaaatt ataatttttt tgaaaatttt attttttata atttgtgaat tttttattat
    40741 agaaataaat atgttattat tatgtcaaaa tttagcatta gattcatgta gagtatgttt
    40801 tatacatata ttataattaa ttctttgaag attgcatatt caaaatacac accatttttt
    40861 ttaattctat aatataatat ttatatattt aatgttagga tgtgatttaa ggtgtatttt
    40921 acatatatta taagatgtaa agatgattct ctatacactg gatataccag taatatagtt
    40981 agaagaatga atgaacataa attaggaatt aattcaaaat acacaagagc aaagggattt
    41041 gaaaaactag aagtttattt tgtgaccaac acaaaaagca atgctatgaa gttagaatat
    41101 tatataaaaa aattaactag aaataaaaaa ttgtctataa taaaaaaccc aagtatactt
    41161 ataaatttaa ttgataataa agaagattat attattggta aggaaataga acaattaaca
    41221 taaatattta tagtaacatt ataatgggtg catatggtgt aaaaacttga caataaatac
    41281 tattgtaata taatgaagtt ataaatatag taaattaaaa tagctagggg agccaagagc
    41341 tgagaggtat accgaccctt acacctgatc tggataatac cagcgtagga aagcttagta
    41401 aaaattcaag tatgtattat ttgtgatata tgctatttct aagctatacc tattgtaggt
    41461 atagcttttt taattaagtt atttaaatta aactttagga ggcaacaatg agtaattata
    41521 aaataccaac acttacaata gccggctctg attcatctgg aggagctggg attcaggctg
    41581 atttgaaaac gtttagtgca attggtactt atggtatgag tgtaataaca gctataacag
    41641 cacagaatac taaaggagtt tttgctgtag aggatttaaa taagaaaata ataaaaaaac
    41701 aaatagaggc agtctttgaa gatatacctc caagagctgt aaaaatagga atggtttcaa
    41761 gtcctgagat tatacttgaa atagttgaga atctaaagaa atacaatcca aaatatttgg
    41821 ttgtggaccc agtaatgata tctaaaagtg gatattatct attaaagcca gaggcaaaag
    41881 aaaacttaat taagtatttg ataccacttg catacataat tacaccaaat ataccagagg
    41941 cagaagaaat aacaggaata aaaatacata atgtagatga tatgaagaga gtaggagaag
    42001 aaatactaca acttggacct aaatttgtac ttatgaaagg gggtcattta gatggagaag
    42061 cagtggatat tttagttggt aaaaatatat ttaaagttta taaaagtgaa agaattgata
    42121 aaaaaaatac acatggaaca gggtgcacat tatcttctgc aataacatct tatctagcat
    42181 taggatatga aataacagaa gcagtaaatc taagtaaaat ctatataaca gaagcaataa
    42241 aaagaagttt tgatataggt catggagtag gaccagtaca tcatttttat aaatttgagt
    42301 aataaaaaaa tattggaggt agaatatgta taatttaatt aaagatgtaa aaaaattaaa
    42361 tccattggta attcattata ctaataatgt aactataaat gattgtgcta atgtaacttt
    42421 agctgtagga gcaagtcctc ttatgagttt ttcatatgag gaagtagaag aaatggtaag
    42481 tgtagcaaac tctgtagtaa taaatatagg aactatgaac tcaaatatgt tggatttatt
    42541 cctattagct ggaaaagcag caaataagta caataaacct gttgttttgg acccagttgg
    42601 agtatttgca agcaaagcga gagcagaact tacaagtaga cttttaaatg aagtcaaatt
    42661 tagtgttgtt aaagggaatg tatcagaaat aaagtttata ggtggcttta atgtaagggg
    42721 taaaggtgta gattcatttg atgaagaaga agattctact gagataataa gaaagattgc
    42781 tgaaaagctt gaatgtgtag ttgtagcaac tggaaaaata gatataataa ctaatggtaa
    42841 aggtacatat aaaataaaca atggaactga taaattaaaa ggaattacag gaactggatg
    42901 tatgactgct agcttgattg caagttttat ggctgttact gagaatatat tagaagcagc
    42961 aacaatgggt gttcttacaa tgtctttaag tggggaatta gctaatttaa ataatccacc
    43021 aataggtaca tttaaagaaa atcttatgaa tgctatttat caaatggata tagatacttt
    43081 aagtaaaaat tctaatattg aatttttaaa ttaataaaga gtaagggaga cttaaaatga
    43141 ttgataaaga atctttaaaa aaatgtttaa agttgtattt agtaactgac tctgaaatgc
    43201 ttaaaggtag agacttttat aaatgtcttg aagatgcaat atcttctgga ataacaactg
    43261 tacaacttag agaaaaaaat gcttctggaa gagagttttt aagaaaagct atgaaactta
    43321 gagaaataac taaacaatat ggtgttaagt ttataataaa tgatagagtg gatatagcac
    43381 ttatttgtga tgcagatgga gtacatgtag gacagagtga cattgatgtt agagaggtta
    43441 gaaagcttat aggaaacaat aaaattctag gagtatcagc aagaacttta gaagaagcta
    43501 tttgtgcaaa aaatgatggc gcagactatt taggtgtagg gtctatattc acaacatcta
    43561 ctaaattaga tgcaaaatca gcttcgtttg aaactgttaa agaaattaaa gaaaaagtag
    43621 acatgccatt tgtactaatt ggaggtataa atttagacaa tatagataaa cttaaatgtt
    43681 tagaaagtga cgggtatgct ataatatcag ctatattaaa agctgaagat atctctaaag
    43741 aagtggaaaa gtggacatta aaaatataaa attggctctg gtagtgaaaa aaatggaaaa
    43801 atttgatata atctaagagt aaaatttaag caattataaa tgataaactt ttctacatat
    43861 actttttaat caagatatat gtagatttgg ttaaaaagga gctataaatg attaggatat
    43921 tgttagtgtg tgttggaggc atgtcttcta ccctattggt aaataaaatg gagaaggatg
    43981 caaaaaaaag aaatatagac tgtaaaatat gggcagttgg agaaggagat ataaaatcgg
    44041 agcttgataa ctttgatatt ttacttcttg gaccacaact tagatttatg cttgatgacg
    44101 taaagtctat agttggagat agagctccag tatctataat agatatggta aactatggca
    44161 cttgtaatgg tcatgctgtg ttaaattcgg ttttagaaat attaaaataa ataataaaag
    44221 tggattctat ataatagagt ccatttttat tatttgttat taataggctt aaaacttagc
    44281 ttaccatttc tttttttaat ctccacatag caagtgaaaa acttgaacag cacaatatta
    44341 aaaatagtag gataacaggc agtatatctc caataagaat agtctcacca aatattaagc
    44401 tacgtaaagc gttaataaca tgggtaaatg gattgagcat cactgctact tttaatcctc
    44461 cagataaact ttctataggg aaaagagctg aacttaaaaa gaaaatagga agtacaattg
    44521 cagtcataat tgtttcataa actatttcat ttggcaataa taaacttata gaataagcta
    44581 gacttgataa aaagaaggct gtcataaata tcagtactat cataagtagg attccaccaa
    44641 tgccagattc tatccttact gagaagaata tacttactat acacaaaata gttacttcta
    44701 tgaaagaaac taagatagct tctaataatt gaccaaggac gattgaatac ctactgatag
    44761 gtgcaattaa gactctatag aaacttccac tgttcttcat tataaagttt ataataccac
    44821 cactactaca agaagaaaac acaaccagta caatgatacc tggtagtata aaagcagtat
    44881 aattattgat atttatatta ttcatagttt ggtttgcaat agaactataa agcacaagcc
    44941 aaagaaaagg ttgtaatata gtaactacaa atgagattgg atttttaaaa cgccacttca
    45001 tactacgcca taagatattt aacattccca ttcctccttt aagctttttt tagaacttgt
    45061 aagtgctaaa aatacatctt cgagacttgg ctctacaatt tctattgcat caaattctat
    45121 atcatgttct aaaagccatt tatttattaa agtaaaagca gttctactat cgtttacact
    45181 tataaaaatt gaattttctc gtacgctcat aaactttact aaaccagtac ttttaattga
    45241 atctttatat ttttttatat cttcagtatt atgaaaagtt atacgtagta tattttgtct
    45301 aatgtaacta cgtaaacttg atggtgttcc ttgtgctaaa tcctttccat tcttcataat
    45361 acaaatatta tcactcaatt gttcagcttc ttcaagataa tgagtagtta gaaatatagt
    45421 tgtgccatac tcatctctaa tttttaataa catatcccac atagattttc ttgaatcgac
    45481 atccatacca acagttggtt catctaaaaa taggatttta ggagatgata ccatattcat
    45541 agctatgtcc aatctacgtt ttacaccccc tgaatatgat gaagtaggat attttaagta
    45601 actagataag tcaaacttat caattaaaga gtctattctt tgttttgcaa tctgtggttc
    45661 tactttatac attttacttt gaaataccat attttccata agggataaat gttcatcaat
    45721 agagatttgc tgtgctacac aagcaatttg agtacgtatc caagaaggat tatctactaa
    45781 atcttttcca aacattgtta catttccaga tgtaggtttg taaaaggtag tcaaaatatt
    45841 tataagagaa gatttaccag caccattagg accaagtaaa gaaaaaattt ctccagcatt
    45901 tacattaaag cttagaccat ttaaagcttt cacaccattt ttatattctt ttactaaatt
    45961 gtctatttca atcgctaaca tatattattc ctcctcttta agtggaaata ttatctctgt
    46021 aatatagtta tctgggtttc cttttatcaa catacctggt cctttaataa atacttctct
    46081 ccaaggagtt tgtattttta tattattttc atgaatatat ttaaatatca tttcatacgt
    46141 ttttggcaaa ttactataag agcctgtgtg agtaaaaaat aaagcttttg ttttaggaaa
    46201 ttcttttgta gttataccca tacgatttgg agtttcagca gttggaacac ataattccat
    46261 ttctgcaaca ccagtttttt ggtcaacatc atagtagcaa aagaatggtg caccgttaga
    46321 ctttcctttt atagctttaa atacatttgg aaaatacttt gttgcctcag tcataggtcc
    46381 attataacgc atggtagcca ctgtgactga ttcgatattt ttgatttcaa ttttgcaatt
    46441 catttgttta cctcctaatg tgtgttcata ttcttactag atttttaagc attaatacat
    46501 ataagatttt gattagttga aaacatataa tcttatctcg tttgcttgtt ttcaagtcta
    46561 acataacata aaatatatat cctctcattt gatgctccat tttgtaaagt tattatttaa
    46621 gctttacttt agatttttta gcaaattaaa ataaagcaat ttataaaata agtatttaca
    46681 aattaagtgt attaatcgta taatacactt aataggatac tagataaagt ctgaaataaa
    46741 ggcgtatttt tttaaagtaa gtgtactatc gtattaatac actagaatag gaggaatcat
    46801 gaattttgaa ttagataata caactcccat ttatttgcaa atagtaaagt atataaagag
    46861 acaaatagta acaggagagt tgaaaccagg agagacaata ccatcaagaa gagaaatggc
    46921 acttaatcta aaagtaaact taaatacagt tcaaagagca tataaggaga tgggtgatat
    46981 gaatataata aatactttta aaaattatca aagcagtgta acagttgatg aaaatatatt
    47041 aaaaaattta aaattagagc ttataaatga atcactatct gtttttatag aagatatgaa
    47101 agcaataaat gttagcaaag aagaagtttt aaaaataata gaagataagt attagttttt
    47161 taacaaatag aaaacttagt ttagaaagtt tatttataag catattggat gtggaggata
    47221 gtaaatatga tagaaataaa gaatgtcagc aaaacatata aaaggatgca agggcttaaa
    47281 ataagaaaga tagaagcttt aaaaaatgta agttttaata tagaaaaggg aaaaataact
    47341 gcattacttg gtataaatgg tgttggtaaa tcaactatgt taaaagcaat agcaggactt
    47401 ataaacattg atagtggaga aattcgtata gatggagaaa aaataaatga aaaagtatac
    47461 aataaattgg cttttgttcc agatgtacaa tctcacttta gcaatacaac aataaaagaa
    47521 acatttgaat ttatggaaat tttttattca aagtggaata aagaaaaatc aaaagaaatg
    47581 atggacatat ttaaattgga tgaagatgaa ataattgata acttgtcaaa aggaaacatt
    47641 gcaagagtaa aattaatact tggattttgc caagacccag agtacatact tcttgatgaa
    47701 ccatttacag gaattgattt gtttaagagg gaagagttta taggagtaat agcgcaatat
    47761 atggaggaaa atcaagccat tatcataact actcatgaga tagtagagat agaaagttta
    47821 gttgatgagg ttgtaattct tgatgaaggg cagattataa cttcatttaa tgcagaagaa
    47881 ttaagagaaa gagaaggtaa atctatatta gataagatga gggaggtata taagaatgaa
    47941 taaacttcta tatctaataa atgtagattt gagaagaagc aataagtttt atatagctta
    48001 tatatcattt ttttcattaa ttacacttag tttaaattta ttcgaaatta ataaatttaa
    48061 aaattctaaa tttattatga acttagcaat agaagatttt ggaggtatat tttatggact
    48121 agggatactt agtaatcttg gttttatcca aagtatattg ttctttggga tattgggact
    48181 ttttatatac tatgttttta tgtggaaaag agagttcttt tcaaataatc aaagtattta
    48241 tacacttatg atgttacctc aaaataagtt taagatttgt atatctaaat caatagcctt
    48301 gataacaatg atatatggtt ttttgataag tcagacagca acattattta tatgtaaata
    48361 tatatttaat ttcattttta gaaatatgcc tataatcaat atgaactttg ccaaagattt
    48421 atattattta aacttgaaga gtataccaat tgattttatc tcttttatgg ctatttatgt
    48481 atttttacta cttatagcta tttctattgt aaattgctgt atattattta tcttttccta
    48541 tataaataaa ttttatgtaa ttttaatcac tttgctgatt atagcaggct tatatttttc
    48601 aacaatatat ataaatataa tca
//
LOCUS       DAMJVI010000018        45748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.018, whole genome shotgun sequence.
ACCESSION   DAMJVI010000018 DAMJVI010000000
VERSION     DAMJVI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.018"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            55..171
                     /gene="rrf"
                     /locus_tag="PC545_001120"
     rRNA            55..171
                     /gene="rrf"
                     /locus_tag="PC545_001120"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            179..253
                     /locus_tag="PC545_001121"
     tRNA            179..253
                     /locus_tag="PC545_001121"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Asn,seq:gtt)
     gene            259..344
                     /locus_tag="PC545_001122"
     tRNA            259..344
                     /locus_tag="PC545_001122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:293..295,aa:Leu,seq:taa)
     gene            360..435
                     /locus_tag="PC545_001123"
     tRNA            360..435
                     /locus_tag="PC545_001123"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:393..395,aa:Met,seq:cat)
     gene            443..517
                     /locus_tag="PC545_001124"
     tRNA            443..517
                     /locus_tag="PC545_001124"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:476..478,aa:Glu,seq:ttc)
     gene            532..605
                     /locus_tag="PC545_001125"
     tRNA            532..605
                     /locus_tag="PC545_001125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Gly,seq:tcc)
     gene            611..686
                     /locus_tag="PC545_001126"
     tRNA            611..686
                     /locus_tag="PC545_001126"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:644..646,aa:Val,seq:tac)
     gene            692..768
                     /locus_tag="PC545_001127"
     tRNA            692..768
                     /locus_tag="PC545_001127"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:726..728,aa:Asp,seq:gtc)
     gene            779..853
                     /locus_tag="PC545_001128"
     tRNA            779..853
                     /locus_tag="PC545_001128"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Gly,seq:gcc)
     gene            863..936
                     /locus_tag="PC545_001129"
     tRNA            863..936
                     /locus_tag="PC545_001129"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:897..899,aa:Arg,seq:tct)
     gene            1920..2759
                     /locus_tag="PC545_001130"
     CDS             1920..2759
                     /locus_tag="PC545_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDF2455908.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            2756..3937
                     /locus_tag="PC545_001131"
     CDS             2756..3937
                     /locus_tag="PC545_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455909.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMSDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVYGTIEDPIQGQKEVY
                     LKANIPQGVTLEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKRNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVSLNDNKDNDVLKNYKLSKEQVTIKGKKEDIDKISYIKTKPIDLADLANGE
                     EKEVSLEIPEGITLEDSSITIKLNDTKQLSAEFLYNAGDIELRNIPTNMDNPNVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            4241..5143
                     /gene="ptb"
                     /locus_tag="PC545_001132"
     CDS             4241..5143
                     /gene="ptb"
                     /locus_tag="PC545_001132"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HDF2455910.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            5165..6244
                     /gene="buk"
                     /locus_tag="PC545_001133"
     CDS             5165..6244
                     /gene="buk"
                     /locus_tag="PC545_001133"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2455911.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            6234..6911
                     /locus_tag="PC545_001134"
     CDS             6234..6911
                     /locus_tag="PC545_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2455912.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6954..7169
                     /locus_tag="PC545_001135"
     CDS             6954..7169
                     /locus_tag="PC545_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2455913.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            7198..8277
                     /locus_tag="PC545_001136"
     CDS             7198..8277
                     /locus_tag="PC545_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2455914.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGKLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            8277..9029
                     /locus_tag="PC545_001137"
     CDS             8277..9029
                     /locus_tag="PC545_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2455915.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            9030..9587
                     /locus_tag="PC545_001138"
     CDS             9030..9587
                     /locus_tag="PC545_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2455916.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            9757..11103
                     /locus_tag="PC545_001139"
     CDS             9757..11103
                     /locus_tag="PC545_001139"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDF2455917.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCLPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQRAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSEIASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            11370..13202
                     /gene="glmS"
                     /locus_tag="PC545_001140"
     CDS             11370..13202
                     /gene="glmS"
                     /locus_tag="PC545_001140"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDF2455918.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEKEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELISEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIILDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKEIPSKIQEIL
                     DNEEYIKEVAKTVVSSEHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAAGELKHGT
                     IALIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNKDVEKAADKIIYI
                     PNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
     gene            13653..14948
                     /locus_tag="PC545_001141"
     CDS             13653..14948
                     /locus_tag="PC545_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HDF2455919.1"
                     /translation="MTNDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDRKDEVLEQALKTKRIDLDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFEDSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLIKFALD
                     YGLTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFILVDNRTWDEHIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNRQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            15398..16135
                     /locus_tag="PC545_001142"
     CDS             15398..16135
                     /locus_tag="PC545_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HDF2455920.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTKYNRLSKALEISEAN
                     FKFYNVKINSVINHNLSNKEFHNIFIEIINNLKLDKKGINWNQKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            16156..17409
                     /gene="murA"
                     /locus_tag="PC545_001143"
     CDS             16156..17409
                     /gene="murA"
                     /locus_tag="PC545_001143"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDF2455921.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            17496..18560
                     /gene="spoIID"
                     /locus_tag="PC545_001144"
     CDS             17496..18560
                     /gene="spoIID"
                     /locus_tag="PC545_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HDF2455922.1"
                     /translation="MKNPLVVLLGFVTCSVLVPSLITLVSYKNVELTEKAESPVSINK
                     TIKKSDIEDKGNKEEKNTVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            18626..19294
                     /locus_tag="PC545_001145"
     CDS             18626..19294
                     /locus_tag="PC545_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HDF2455923.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVSVKKEQKVTEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            19392..19652
                     /gene="spoIIID"
                     /locus_tag="PC545_001146"
     CDS             19392..19652
                     /gene="spoIIID"
                     /locus_tag="PC545_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HDF2455924.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            19849..20868
                     /locus_tag="PC545_001147"
     CDS             19849..20868
                     /locus_tag="PC545_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDF2455925.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDNRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            20884..21312
                     /gene="fabZ"
                     /locus_tag="PC545_001148"
     CDS             20884..21312
                     /gene="fabZ"
                     /locus_tag="PC545_001148"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDF2455926.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRRNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(21423..21932)
                     /gene="yyaC"
                     /locus_tag="PC545_001149"
     CDS             complement(21423..21932)
                     /gene="yyaC"
                     /locus_tag="PC545_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HDF2455927.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     regulatory      22121..22224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            22360..23553
                     /locus_tag="PC545_001150"
     CDS             22360..23553
                     /locus_tag="PC545_001150"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDF2455928.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGLVDYLRPDGKTQVTVEYEGSKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVAAGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     gene            23868..24965
                     /locus_tag="PC545_001151"
     CDS             23868..24965
                     /locus_tag="PC545_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455929.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     IGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGTFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMTIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCMLYRRITKTEGYKENKSEIS"
     gene            25229..27448
                     /locus_tag="PC545_001152"
     CDS             25229..27448
                     /locus_tag="PC545_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDF2455930.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKIYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIEVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELNGEIIKECVMMLVYNQKIHVEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEEDKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMINSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYILFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPIAWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            27451..28233
                     /locus_tag="PC545_001153"
     CDS             27451..28233
                     /locus_tag="PC545_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDF2455931.1"
                     /translation="MKICNDNFIKNFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIRHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYILFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKDNVKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            28565..30478
                     /locus_tag="PC545_001154"
     CDS             28565..30478
                     /locus_tag="PC545_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2455932.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNKIANDFECSERTIRNDCKY
                     IDEWLSTFCNAYIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEISKLSVALNNYNLNVSTKKGSGIYIGGDEKDI
                     RIMLVSFLFKYIDKYRLNIDEIEYFHIVDIIIVKNIMFTIEEDMDIRLTDISFKQLML
                     FLLINIIRIRLGNSLKVSNTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLIL
                     GMNKQINSSNIKKSLYLNEELASYTKKIINLVSEESGINFNNDILLYEQLICHLNVTM
                     HQMKSNVYLENPLLDNIKTKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLE
                     RKYSKRESNKKASIVCPFSFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTID
                     FIIAFQEYENIQKPIFITTPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIR
                     ELEHDDIYEAITYLTNLLIEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIK
                     QSVLCFARLKTPIRIRNGKDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDIL
                     SKCDIEKVKDILI"
     gene            30619..30927
                     /locus_tag="PC545_001155"
     CDS             30619..30927
                     /locus_tag="PC545_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2455933.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            30951..31277
                     /locus_tag="PC545_001156"
     CDS             30951..31277
                     /locus_tag="PC545_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2455934.1"
                     /translation="MKRKVYLFCSFGMSTSLLADKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            31316..32650
                     /gene="celB"
                     /locus_tag="PC545_001157"
     CDS             31316..32650
                     /gene="celB"
                     /locus_tag="PC545_001157"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDF2455935.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     ANFPIKGWNEFVGQIFGSDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSIHEFIFKFL
                     QMPLLKLGNSLGAMAIAYIFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGS
                     KIPNIITGQFQDMFATFGGAGSTLSLVIAMLLICKSKRIKSMGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFAIVPTINIIIAYFCMSAGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQVVLLILGIFMYIPFIKMLDKQYLREENATVEEEDDDISFDDLVL"
     gene            32661..33179
                     /locus_tag="PC545_001158"
     CDS             32661..33179
                     /locus_tag="PC545_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HDF2455936.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGIMMEQFLNE
                     SKVIATLFCGDEFFVNNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKTDIPAFAAMSEENIDVINEYKNDICIVKTPKKGGIGLNDSLNNICKLAKAIANK
                     EDITLMKEEFCY"
     gene            33275..34234
                     /locus_tag="PC545_001159"
     CDS             33275..34234
                     /locus_tag="PC545_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HDF2455937.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEDYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            34283..35335
                     /locus_tag="PC545_001160"
     CDS             34283..35335
                     /locus_tag="PC545_001160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDF2455938.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKVRNLDNYIGYAEHGDSDDYVCVIGHVDVVHEGDGWKHQPYKGE
                     ETNGRIYGRGVLDNKGPIMSALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVYLGFIENMSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKASFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            35338..36012
                     /locus_tag="PC545_001161"
     CDS             35338..36012
                     /locus_tag="PC545_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDF2455939.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIESV
                     VNSVKIPVNVMIRHHAKSFVYSKEDISIMQKDISVVKEIGANGVVFGVLDKNNSIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKILKDYDKITNILTSGGKGSIVDNIQMIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRINNSPFEDIDRQKLKQL
                     VNIISR"
     gene            36565..37116
                     /gene="raiA"
                     /locus_tag="PC545_001162"
     CDS             36565..37116
                     /gene="raiA"
                     /locus_tag="PC545_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2455940.1"
                     /translation="MNIIISGKQIELTDAIKNKIETKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            37248..39923
                     /gene="secA"
                     /locus_tag="PC545_001163"
     CDS             37248..39923
                     /gene="secA"
                     /locus_tag="PC545_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2455941.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPTSKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            39938..41045
                     /gene="prfB"
                     /locus_tag="PC545_001164"
     CDS             join(39938..40012,40014..41045)
                     /gene="prfB"
                     /locus_tag="PC545_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDF2455942.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEDDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            41189..43330
                     /locus_tag="PC545_001165"
     CDS             41189..43330
                     /locus_tag="PC545_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDF2455943.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLEKLALSILENNLDNIEIEAKNYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMPPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVSSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DNEVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHVAGISKTIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNICEIDEKIKEIGLRELSEKLEVGQVTLKDIITEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMTLKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            43478..44644
                     /locus_tag="PC545_001166"
     CDS             43478..44644
                     /locus_tag="PC545_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2455944.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIDEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPIDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTCGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIVDELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVLYTIINGKVVYEKK"
     regulatory      44704..44821
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            44945..45550
                     /locus_tag="PC545_001167"
     CDS             44945..45550
                     /locus_tag="PC545_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2455945.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPFVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVILSLVTLPLYKKMGNVIKK"
BASE COUNT        18389 a         5018 c         8755 g        13586 t
ORIGIN      
        1 ctaggttcta taaacttatt aaaaacttaa tagaaatatt aagcataaag attatgtggt
       61 tacaatagca gagaggatac acctgttccc atttcgaaca cagaagttaa gctccctagc
      121 gctgatggta cttggtggga aactgcctgg gagagtagga cgtagccacg ttaaatgttc
      181 caaggtagct caacggtgga gcaaccggct gttaaccggt aggctgtggg ttcgagcccc
      241 acccttggag ccaaaaaagc cgaagtggcg gaactggcag acgcacagga cttaaaatcc
      301 tgcgggactt acctctcgta ccggttcgat tccggtcttc ggcacttata aaacaatatc
      361 gcggggtgga gcagttggca gctcgtcggg ctcataaccc gaaggtcgta ggttcaagtc
      421 ctgcctccgc aaccattaaa acggctcgtt ggtcaagagg ttaagacacc gccctttcac
      481 ggcggtaaca ggggttcaat tcccctacga gtcaccaatt caattaaata tgcgggtgta
      541 gctcaatggt agagttctgg ccttccaagc cagctgtgag ggttcgatcc ccttcacccg
      601 ctccaaaaat gggaccatag ctcagctggg agagcacctg ccttacaagc agggggtcac
      661 aggttcgagc cctgttggtc ccaccattcg cggcctggta gttcagttgg ttagaatgcc
      721 agcctgtcac gctggaggtc gagggttcga gccccttcca ggtcgccaaa ttaaataagc
      781 gggaatagtt cagtggtaga gcgcaacctt gccaaggttg aagtcgcgag ttcgaatctc
      841 gtttcccgct ccataataac ttgggtgcat agctcagctg gatagagcaa cgcccttcta
      901 aggcgtgtgt ccggggttcg aatccctgtg cgctcattaa gaaaagttta ctataaatta
      961 tagtaaactt ttatattgtt taaaaagaca caggaaacat gtatattacc tgtgtctttt
     1021 tatttataaa ctttaaacta tgtaaaaatt acagtatatt ctaatagagt tacagtatat
     1081 tcattaatat tgtaaacata cggagggtaa tgtataatag tctacaagat gtttatgaag
     1141 gcatcagtta aatataataa atataactaa ttagtaagag ttatatacat tatattgtat
     1201 actttatggt atttatatat tgaaataact tatgaaatta tttataaaga gattcatgag
     1261 catatttttg tctaaacaat aagtataaga aattataaaa agttagagac tatagtgaat
     1321 ttacaattta atgtaatatt tataagttat actaaataaa ttattatact gtgaaatgga
     1381 tatataactt taggtattgt gtattatttt cactgtagga gattatatct agttatacat
     1441 aataagtttt aatgatagtt tatagtaaga tattaaaata aatttatcta atctagatat
     1501 gaaataagtt attactttaa tataaaaatg aatctaatgg aataatattg atgataagta
     1561 atatttagat tttgtatttg tttatagtta ataagtggat tatagaattt ataataatta
     1621 atatatatta aacaaagata tattaagata ttttagaaca taaaactgaa ttaaggcgcg
     1681 agtactattt tgtattcgcg ccttgatggt ttgagttatt tgagtaaaaa ctaactttaa
     1741 taagaaatat gtatttatta agttgatatt tttaatgtgt ttagagatta tttaacaatc
     1801 tccaaatata aaactagaat tatttacata aaagttaaat agtttgtata gagttagatt
     1861 tatatagtat aatacttagt gtaaataatt aagaagttat caaacaggag gataaaactg
     1921 tgttagatat aaattctttt ttaaatggat tttttaatat aatattaaga atgagtatat
     1981 atgatttaat tgacatatct atagtagcgt atatttttta caaaatattt atgtttataa
     2041 aagatacaag agcagaacaa gtgtttaaag gtataatatt tcttttatta gcaactcaac
     2101 ttagtaacac ctttaaattg cataccgttt attggatatc tcttaaagct ttagattatg
     2161 gagttatagc tgctcttata atttttcaac cagaatttag agcaggactt gaacatatag
     2221 gaagagcaaa gtttaattta tttggaaaga atgtaaatac ttctgaagaa actctcaata
     2281 gaaatataga agaaattgta gaagctttat attcactttc tagacaaaaa ataggagctc
     2341 ttatcataat ggagagagaa actagaataa gtgatataat aaatacagga actattattg
     2401 atgctgaaat ttctaggcaa ctattaataa atatatttat accaaataca ccattacacg
     2461 atggagctgt tgtaattaga gattctaagg taaaagcagc tgcatgtttt ttgccattga
     2521 cagaaagtaa ggatttaagt aaagatttgg gcactagaca tagagcagga ataggggtaa
     2581 gtgaggtgtc tgactgtata actttgattg tatcagagga aacaggtggc gtatcaattg
     2641 ctaaagcagg taagttatat agagatattt ctagagaaag aatgatgaat atattacgta
     2701 gtaatttaaa aacaaataca gaaacaagaa gttttttcaa aggtggtata tttaaatgat
     2761 aaatagacta aaaaataata ccaaaataaa aataatatca tttttaagtg caattgcatt
     2821 atggatgtat gttatggctg tagttgaccc tgaagaaact aaagggtttg aagatatacc
     2881 tgtatctata agtaatatga gtgatttaaa ggataaaaat cttgttatat atccagacga
     2941 aaaattaaca gcagatgtat atataaaagg tagtttatca aaggtacaaa gtgttaaaaa
     3001 agaagatata catgtatatg gaaccattga agacccaata caaggacaga aggaagtata
     3061 tttaaaagca aatattcctc aaggtgttac tctagaattt aagcatgatg ttttagtagt
     3121 aaatcttgaa aaaaacataa aagaaaaaag agatataaag gtagaagtaa gtggaaattc
     3181 aaaaagaaat ataaataaaa ttgaacttag taaggaattt gtcgatgtat caggtccaag
     3241 aactctagtt aaagaagtca aaagtgtaag agctaatcta gatgttggta atgagacaga
     3301 tgatttttct aaaaaattgc aattaatacc attaaatagt gatggaacta aggttgaagg
     3361 agtaacatta agttcatcct ttgtgactgc aaagatagag ctattaaagg aaaaaattgt
     3421 cccaataaga gtaagtctaa atgataataa agataatgat gtactgaaaa actataaatt
     3481 aagtaaggaa caagttacaa taaaaggaaa gaaagaagat atagacaaaa taagttatat
     3541 taagacaaaa cctattgact tagctgattt ggctaatggt gaagaaaaag aagttagctt
     3601 agaaattcca gagggtataa ctcttgagga tagctctata acaattaaat taaatgatac
     3661 aaaacaattg agtgcagaat ttttatataa tgcgggagat attgaattaa gaaatatccc
     3721 aacaaatatg gataatccaa atgtaatttc tgatgtagat ataaaagttg ttgtagagtc
     3781 aacggaggat ttgagtaagt taaataaaaa agatatttca ttatatattg atttatcaca
     3841 aggtcctgag gaagatagta aatataaaat aaaatatgaa acaacatatc aatttaaaaa
     3901 aataaatata gaacctaata ttgttgaagt ggaatgaaca aatatttcga atatttagtt
     3961 gttacaagat gcgacaaaaa aacctataaa attaagtttg ttaattttat gtttttgttg
     4021 catcttgttt attttagtta tattataaaa taaggacaat gagatatttg ttaaatctaa
     4081 aaagcgctag atatatgaaa aaagactgat aaatagattt aaataaatat tttgaattaa
     4141 atatttcaat tatctctagg aaaacgttgc tgaacgagtt ataatttaat tgagatataa
     4201 aaagttctat ttcaaaaaat tcaagatgag gtggaaatgc atgagaagtt ttgaagaagt
     4261 aattaagttt gcaaaagaaa gaggacctaa aactatatca gtagcatgtt gccaagataa
     4321 agaagtttta atggcagttg aaatggctag aaaagaaaaa atagcaaatg ccattttagt
     4381 aggagatata gaaaagacta aagaaattgc aaaaagcata gacatggata tcgaaaatta
     4441 tgaactgata gatataaaag atttagcaga agcatctcta aaatctgttg aattagtttc
     4501 acaaggaaaa gccgacatgg taatgaaagg cttagtagac acatcaataa tactaaaagc
     4561 agttttaaat aaagaagtag gtcttagaac tggaaatgta ttaagtcacg tagcagtatt
     4621 tgatgtagag ggatatgata gattattttt cgtaactgac gcagctatga acttagctcc
     4681 tgatacaaat actaaaaagc aaatcataga aaatgcttgc acagtagcac attcattaga
     4741 tataagtgaa ccaaaagttg ctgcaatatg cgcaaaagaa aaagtaaatc caaaaatgaa
     4801 agatacagtt gaagctaaag aactagaaga aatgtatgaa agaggagaaa tcaaaggttg
     4861 tatggttggt ggaccttttg caattgataa tgcagtatct ttagaagcag ctaaacataa
     4921 aggtataaat catcctgtag caggaagagc tgatatatta ttagccccag atattgaagg
     4981 tggtaacata ttatataaag ctttggtatt cttctcaaaa tcaaaaaatg caggagttat
     5041 agttggggct aaagcaccaa taatattaac ttctagagca gacagtgaag aaactaaact
     5101 aaactcaata gctttaggcg ttttaatggc agcaaaggca taattttaag ggggaaatag
     5161 gcaaatgagc aaaatattta aaatcttaac aataaatcct ggttcgacat caactaaaat
     5221 agctgtattt gataatgagg atttagtatt tgaaaaaact ttaagacatt cttcagaaga
     5281 aataggaaaa tatgagaagg tgtctgacca atttgaattt cgtaaacaag taatagaaga
     5341 agctctaaaa gaaggtggag taaaaacatc tgaattagat gctgtagtag gtagaggagg
     5401 acttcttaaa cctataaaag gtggtactta ttcagtaagt gctgctatga ttgaagattt
     5461 aaaagtggga gttttaggag aacacgcttc aaacctaggt ggaataatag caaaacaaat
     5521 aggtgaagaa gtaaatgttc cttcatacat agtagaccct gttgttgtag atgaattaga
     5581 agatgttgct agaatttctg gtatgcctga aataagtaga gcaagtgtag tacatgcttt
     5641 aaatcaaaag gcaatagcaa gaagatatgc tagagaaata aacaagaaat atgaagatat
     5701 aaatcttata gttgcacaca tgggtggagg agtttctgtt ggagctcata aaaatggtaa
     5761 aatagtagat gttgcaaacg cattagatgg agaaggacct ttctctccag aaagaagtgg
     5821 tggactacca gtaggtgcat tagtaaaaat gtgctttagt ggaaaatata ctcaagatga
     5881 aattaaaaag aaaataaaag gtaatggcgg attagttgca tacttaaaca ctaatgatgc
     5941 tagagaagtt gaagaaagaa ttgaagctgg tgatgaaaaa gctaaattag tatatgaagc
     6001 tatggcatat caaatctcta aagaaatagg agctagtgct gcagttctta agggagatgt
     6061 aaaagcaata ttattaactg gtggaatcgc atattcaaaa atgtttacag aaatgattgc
     6121 agatagagtt aaatttatag cagatgtaaa agtttatcca ggtgaagatg aaatgattgc
     6181 attagctcaa ggtggactta gagttttaac tggtgaagaa gaggctcaag tttatgataa
     6241 ctaatgataa aagaataaga atcataactg gtcactatgg aagtggtaaa agtgaatttg
     6301 cgatgaacta tgttgttaag ttaagagata tggtttctgg caaagttgct atagccgatt
     6361 tagatgtggt aaatgtatac tttagaacta gagaaaagaa agagttaatg aaatccttag
     6421 gtatacaacc aatagatagc tcaattaatg ctccaacatt agatttacca gcagtatcag
     6481 cagaggtaat gtctcctatg gtagaccatt catacaattc tgttatagat ttaggtggag
     6541 ataatgtagg tgctagggtt ataggaagat ttagtcattt gttaaaagaa ggcgattatg
     6601 atatgctttt tgtaataaat gcaaataggg agaaaactca aacttctgaa gaagttatcc
     6661 aatatataaa agaaatagaa aaatcttcta aattaaaggt aacaggtcta attaataata
     6721 ctcatcttat taggtttact actattgatg atgttttaag aggtcagaaa gttgcaaaag
     6781 aagtttctga aaaatgtaat ataccgataa gatatgttgc atgtttagaa aatcttgttg
     6841 aacagttacc aaaagattta gaaggagaaa tttttcctat aaaattatat atgagagaag
     6901 actggatgta gtttgagctc aaatatatag aaagtttaag gaggcttagg taaatggcta
     6961 aaggaaaagt atcttttaat caagaacgtt gtaaaggttg tggattgtgt gttgaagcat
     7021 gtccagtaaa aatcatacaa ttagactcaa atgtaataaa caaaaaaggg tataacccag
     7081 caactgtttt tgaaatggaa aaatgcatag gatgtgcaag ttgtgcaact atgtgtccag
     7141 atgtagttat aacagtagaa agagattaat ttaattaata tttaaggggg aattttaatg
     7201 gctaagatac ttatgaaagg taacgaagca tttggaaaag cagctataga agctggttgc
     7261 aaatatttct tcggttaccc aataactcca caaagtgaat taccagaata tttatcaaga
     7321 gagttaccta aaataggtgg agcgttcgtt caagctgaat cagaagtttc tgcgataaac
     7381 atggtttatg gtggagcagg tgcaggagca agagttatga cttcttcatc ttcaccagga
     7441 gttgctttaa agcaagaagg tataacatat gcagtaggag cagaagttcc ttgtgttgta
     7501 cttaatgtaa tgagaggtgg tccaggactt ggaagtatac aaccttcaca agctgactac
     7561 tttatgtcta caagaggtgg aggaaatgga gactacagaa ctccagtatt cgcaccagct
     7621 acagttcaag aagctgttga tatgataatg gaagcttttg acgttgcaga ttattacaga
     7681 tcaccagtta tggtagttgc agatggtatg ataggtcaaa tgatggagcc agttgaattt
     7741 agagctcctg agaagaaaag agaattgcct cctaaagatt gggcaactgt tggaactaaa
     7801 ggaaaaagaa aacctaatgt aataaactct ctatacttag agccagaagt actagaagac
     7861 cattgttggc atttacaaga aaaatttgat gcaatggaaa agaatgaagt acaatacgaa
     7921 atgtacaaaa cagaagatgc tgaatttgta tttgcagctt atggaactac ttcaagagta
     7981 gtaaaaagcg caattgatat attaagagaa gaaggaataa aagcaggtct tataagacct
     8041 aaagtattat ggccattccc attcgaagca ttcaaccaaa ttcctaatgc tagaaacata
     8101 ttgactgttg aaatgagtat gggacaaatg gtggaagatg ttaaaatggc agtagaaggt
     8161 aaattaccag tttacttcca tggaagacca ggaggaatga ctccaacacc agcagaaata
     8221 gttgaaaaag ctaaaaaaat tattgccgga gaattggttg caggaggtgc tagataatgg
     8281 ctgttgtatt taagaaaact gaaggattac aagatactca aactcactac tgtccaggat
     8341 gtacacatgg tataatccat agattagttg gtgaagtatt agaagaacta ggagtattag
     8401 gtgatgctgt aggtgttgtt ccagttggat gttcagtttt aggatataaa tattttaact
     8461 gtgacactca agaagctgct catggtagag cgccagctgc tgctacagga ataaaaagag
     8521 ttcatcctga aaatacagta ttcacttatc aaggtgatgg agacttagct tcaattggta
     8581 ctgctgaaat agttcatgca gctgctagag gagaaaaaat aactactata tttgtaaaca
     8641 atacaactta tggtatgact ggtggacaaa tggctccaac tacattagtt ggacaaagag
     8701 ctactactgc tcaatcagga agaaatgctg aaactcaagg ttatccaata agagtaagtg
     8761 aaatgttagc tacattaaca ggagctgtat ttgtagaaag agtagcagtt gatactccag
     8821 ctcatgttag acaagctaaa aaagctataa agaaagcatt ccaagttcaa caagcaggat
     8881 taggatttgg aatagtagaa gtattatcta cttgtcctac aaactgggga ctagctccaa
     8941 acgatgcttt acaatggtta agagacaaca tgataccata ctatccatta ggaaatttca
     9001 aaaatgttga agttgaggag gtgaagtaaa tgtctacagc tagagtaata tgtgcaggct
     9061 tcggaggcca aggtgttatg tctatgggac aattacttac ttatgcagga atgctagaag
     9121 gaaaagaagt ttcttggtta ccatcttatg gaccagaaat gcgtggaggt acagcaaact
     9181 gtgcagtaac tgtatctaat gagccagttg gttctccact tataacagat gatgcaactg
     9241 cagctatatt attaaatata ccagcatttg aaaaattcaa agatgatgtt attcctggtg
     9301 gaaaaataat agtaaatagt tctttaataa aagaaaaagt tgatagaaca gatgtagatg
     9361 tttattatat accagcaaat gaattagcag ctgaattagg aaatgataaa gttgctaaca
     9421 tgataatgtt aggtgcttac ttaaaagtat ctgatactgt agatatagaa tctgtactag
     9481 aagcattcaa aaaagttttc ggacctagaa aagaaaaatt cgttccatta aacagagaag
     9541 ctttacaaaa aggtatggat gctacatgtt ctaaagcaac taactaataa agaaaaagat
     9601 gtcaaaaaaa agacaataga attatttcta ttgtcttttt tgctgataag tattaaaata
     9661 tttttatgac tgaaattaag cctataatga tattttatga actataaaaa tataatacaa
     9721 attacaagct tttcaattat gaaaggagaa tctttagtga gaaagtattt tggaacagat
     9781 ggagttagag gagtagcaaa tacagaacta acatgtgatt tagcatataa acttggtaga
     9841 gctggtggat ttgtcttagc tcaaggagac cacagagtaa aagtagtagt aggtaaggat
     9901 actagaatat caggtgatat gttggaagca tctttaatag caggacttat gtctgttgga
     9961 tgtgatgtta ttactgttgg aataatacca acacctgcag tggcatattt aactagaaaa
    10021 tatggtgctg attgtggtgt ggttatatct gcatctcaca atcctgttga atataatgga
    10081 atcaaattct ttaataagaa tggatataaa ttagatgatg aaattgaatt gaaaatagaa
    10141 gaatatatag acgatataga caaaattgat tgtcttccaa ttggtgaaaa tgtaggaaga
    10201 aaattacatg aacattgtgc acaaagagac tatgtagact acctaaaatc aataataagc
    10261 actgacttta agggattaaa agtagttttg gactgtgcta atggtgcgtc atataaagtg
    10321 gcaccaatag tgtttgatga actaggagca agtgtaattt caataaatag ttcaccagat
    10381 ggaaataata taaactataa atgtggctct acacatccag aacaacttca aagggctgtt
    10441 ttggaacata atgctgactt aggtcttgca tatgatggag atgctgatag attaattgca
    10501 gtaaatgaaa agggtcaaat tgttgatgga gaccatataa tgatattgag tgccttaaat
    10561 ttaaagaaaa ataataaatt ggcacaagat actttggtag ttacagttat gagtaatata
    10621 gggttgacaa tagctgctaa ggaaaatgga ataaatttat ctactacagc agttggagac
    10681 agatatgttt tagaagatat ggttaaaaat ggatataatc ttggtggaga gcaatctgga
    10741 catatgatat ttttagatta caacactact ggagatggag tgcttagttc tctaatactt
    10801 gcaaatataa tcttgcaaga aaagaaacct ttatcagaaa ttgcatcaat aatgagccaa
    10861 tacccacaag tattagtaaa tgcaacaata aaaaatgaaa ataagaacaa atatatggaa
    10921 tatccagaaa taaaaactga gatagaaaga atagaatcta ttttagacgg aaatggtaga
    10981 gttttgataa gaccatcagg aactgagcca ttagttagag ttatgttaga gggtaaagag
    11041 gaaggtcaaa taaaagaatt agcaacaaac ttagctaatc ttatacaaga aaaattatcg
    11101 taatttaaag ggtattctca gatataaata ctgaggacac ctttctaagc gccagaactt
    11161 agttatagac ataattattg agtaatatag gaactaagtt gacgaggtca gagttaatcg
    11221 aattgtcggc ggatgctctg cggtgtgttt accatcgaaa gacttcttta aataatagga
    11281 gcaatcctat ggacaaaggg aagccaaagt aaacttatat gacctaactc ttaaaatata
    11341 taaataatat ttttaggagg acacaaataa tgtgtggaat agttggatat ttaggaagta
    11401 gaaaggcagc agaagttata gtagaagggc tttctaagct agagtacaga ggttatgact
    11461 cagcaggagt agcagttaat agttctaatg aaaaagaact taatattaga aagtttaaag
    11521 gtagactttc tgtacttgca gaagacttag aaaagaatcc tattgatgga aatttaggta
    11581 tagggcatac gagatgggca actcatggag aaccatctga tgtaaactca catcctcatt
    11641 ttaatcaagc caaaacaata gcagttgttc ataatgggat aatagaaaac tatatggaaa
    11701 taaaagaaga attaatttct gaaggtgtta agtttgaatc tcaaacagat acagaggtaa
    11761 tagcacattt agtagataaa tactatgagg gtaatttatt agatgctgta tacaaaacta
    11821 tatcaaaatt aagaggagct tatgcattag gtgttatatg taaagaacat ggaaatgaat
    11881 tagttgcagt aagaaaagat agtcctcttg tagttggtgt tggtgaaggt gaaaacttta
    11941 tagcatcaga tatacctgca ttattaaaat atactagaga tgtttatttc ttagaaaatg
    12001 gagaagtagt tcatctaaaa gacgaaaatg taacagttta tgactctaat agaaatcttg
    12061 ttgaaaaaga agtatttcat gtaacttggg atgtagaagc tgcgtctaaa ggtggatatg
    12121 actactttat gtcaaaagaa attcatgagc aaccaacagg agtaagagaa actttagaaa
    12181 gaagacttga tgataatgga aatataattc tagacagtat aaatatttct aaagaagact
    12241 tagaaaaaat aaataaagtt tatatagtgg cttgtggaac agcatataat gctggattac
    12301 ttggtaagta tgccatagaa aaatttgtaa atataccagt aataactgat atagcatcag
    12361 aatttagata tagcgaccca tttgtagatg aaaattcatt agtaatatta gttagtcaat
    12421 ctggtgagac agcagatact ctagcagtat taagagattc aaaggctaaa ggagcaagaa
    12481 tactttcaat aactaacgtt gtaggttcat ctatagcaag agaatcagat gatgtattct
    12541 atacttgggc aggacctgaa gtagcagttg catcaactaa ggcatatact actcaaataa
    12601 cttctttata catgatagct ttagactttg ctatcaaaaa aggaacaata actagagaat
    12661 tttatgatag tatgatttct aagatgaaag aaataccatc aaaaatacaa gaaatactag
    12721 acaatgaaga atatataaaa gaagttgcta aaacagttgt tagttctgaa catgcattct
    12781 atttaggaag aggtatagac tatagcttag caatggaagg ttcattgaaa ttaaaagaga
    12841 tttcatatat acatgcagaa gcttttgcag caggagagct aaagcatgga acaatagctt
    12901 taatagaaaa aggaactcct gtaattgcca tagctactca agaaaagtta tttgaaaaaa
    12961 tggtttctaa tatggaagaa gtaagagcaa gaggagctta tgtagtagca atagctcaaa
    13021 gccataataa ggatgttgag aaagctgctg ataaaataat ctatatacca aattcagatg
    13081 atattttatc accaatatta gcagttgtac caatgcaatt acttgcttat catgtctctg
    13141 tactaagagg atgcgatgta gataagccaa gaaatctagc taaatcagta acagttgaat
    13201 aaaaacagat taaaaattaa atagataata tttattgaaa agaactcatc ctgttatttg
    13261 tattttatag gatgagtttt ttaaataaat attaaatttg ttttgttttt gctataataa
    13321 attttataaa aagattgata ttaaatcatc tgtaaaatga taaatatata gaccatacca
    13381 ggtataggga acgataaata tagttcatat aaaatatagt gagtacttaa taaacatata
    13441 gtcaatatca gataaaaaaa ataatagctt atgagaaaaa ttaaaataca aggtattagt
    13501 aaccttgtga taagataaag tgagtagttt aaaataagaa tataaggaac tatttataaa
    13561 agaatataat gaagctaata aagtataaga aaattacttt attttagtaa aataatttca
    13621 aataaaaatt ttttaaattg ggagtataaa ttatgacaaa tgattttaaa aaagaaaaga
    13681 aagaagatta ttttgttaat cttaaggcaa tatctactga agttttacaa gaaaaagtgc
    13741 aagataacgc ttgggttata gttgatactc gcttaaatga tgcttataat ggctggaagt
    13801 tagatggagt aaaaagaggt ggacatataa aaggagcagt agatttttct gctaattggt
    13861 taagtgtata ctcagataga aaagatgaag tgctagaaca agcattgaaa actaaaagaa
    13921 ttgatttaga taaaaatata gtattatatg atgcaaatgg aaaagatgct ttggttgtag
    13981 cagattattt atctaaaaaa ggatataaat atctatataa atatgatatt aaacaatggg
    14041 cagacaacga aaatcttcca atggaaagat ataaaaatta tcaaatgata gttcctgctt
    14101 ttataataaa agatatatta gatggaaaaa ttcctgagac atttgaagat tcaaagaata
    14161 taaaaatgat tgaagcgagt tggggagaag aatcgtatac aaaagggcat atacctacaa
    14221 gtgttcatgt aaataccgat attatagaac ctcctccaac atggatgctt gataatgatg
    14281 ataacttaat taagtttgct ttagactatg gattaacaaa ggatgatact gtaatagttt
    14341 caagtagtac acctatggca tcttatagac tagctgttat attgagatat ataggagtaa
    14401 aagatgtaag agttttgaat ggtggaacaa actcatggtt atcagcagga tatgaattag
    14461 aatttactag taatccaaaa cattcatgta ctaattttgg agcagatata ccagttaact
    14521 cacaattagt tgttactacc tctgaattaa gacaaaaact aaaagaaaaa aataagttta
    14581 tactagtaga taaccgtact tgggatgaac atataggaaa agtatctgga tatacatatt
    14641 atgataaaaa agggcgtata ccaggagctt tatatggaca ttcaggtagt gatagtgttt
    14701 cactagaaga atatcgtaat attgataata caatgagaaa taaatatgag attttagaaa
    14761 tgtgggacaa ggaaaatata gatgtgaata gacagcttat atttatgtgt ggaagtggat
    14821 ggcgtgctgc tgaagtatta acatacgcaa atgtcatagg agtggaaaat acctcccttt
    14881 atagtgatgg atggatgggc tggagtcttg ataattcaaa tttaattgaa gtgggagaac
    14941 acaaataaag tttttgaaag tacgatagag ttgtttaaaa caaaggtgtt tttgatttat
    15001 tgggaagagt gtgtaaattg tgttttggga agtgtactta ggtatgttca tttggagcta
    15061 tggaaattga taaaaaagta ttatctgttt attttgctgg taaaatgtga atagattata
    15121 gattatagat tatagattat agatttaagt atagactaag taatttatat tacagaagag
    15181 aatatataag aactagtgca agatgtattt gatacatata aaaaattggc aaatccagaa
    15241 taaggataga gaaaattgag attgaagagt ttgaaagaga cttattagat gaaaaataca
    15301 aagatgtata aaaatacata ttgagaaagt atatctctct taaatttgtt aataattcta
    15361 ttatacagaa aaacatattt aagggggatt ttttactatg aaaagagtaa aattagctat
    15421 aagctttttt atattaatga ttatagggat gacaatatct tatgcagata caagtataaa
    15481 tactaaatac aatagattaa gtaaagctct tgaaatttca gaagcaaatt ttaaatttta
    15541 taatgttaaa attaattctg ttataaatca taatttatca aataaagaat ttcataatat
    15601 ttttatagaa ataattaaca atctaaagtt ggataaaaaa ggaataaatt ggaatcaaaa
    15661 agggtctggc gataaaatac aattatgtgc tactataaaa ggtagcgata aagaaatttc
    15721 tataaaagga ataaataaaa ataacaaaga aagttacata gtagttgaca tattggataa
    15781 caaagtatat aaacataaag aggttattta tagtaacgta gaaaatgtgt taaataaata
    15841 ttcatctcaa gtagatgtgt ctgcttgtat tgttggagaa tacacaaaaa ggttacaaaa
    15901 caacaaatat gatgatattt tacaaaaaat attatataat atgagtgcta agcaaataga
    15961 tgaagtgaaa gacgtaaact ttttatctat aacagcatat agtaaagtat taaatgatta
    16021 tgaattagaa tatttagggg aaaaaattaa tttaaatata ggaattaaat ataatgagga
    16081 cgacggaaga acactcatat atattgccac accaattata aaattagatt attaattatg
    16141 aggagatcaa aaaatatggc taagataata gtaaaaaaga gtaatccact taaaggtagt
    16201 gttaaaatag atggtgcaaa aaatgcagtt ttaccaataa tagcagcgac tttattagca
    16261 aacgggaaat caacattaaa tggagtacca aatttaagag atgttcatgt tatatcagat
    16321 ttattaagac atgtaggggc agaagttgaa tataaagaaa atacacttac tgttgatgca
    16381 agtaatataa aaacttgtga agctccatat gagcttgtaa gaaagatgag agcttctttt
    16441 ttggttatgg ggccattgct tgctagattt aatagtacga aaatttctat gccaggagga
    16501 tgcgctatag gaacaagacc tatagattta catttaaaag gatttaaagc cttaggtgca
    16561 aagatagaaa tggaccatgg ttttgttgaa gcagcaacag aaaaattagt aggaaataaa
    16621 ttatatttag atttcccatc tgttggagca actgaaaaca taatgatggc agcttcttta
    16681 gcagaaggga ctacaataat agagaatgca gcagaagagc cagaaatagt agatttagca
    16741 aacttcttga acgaaatggg agcagatgtt aaaggtgcag gtactaatac tataaaaata
    16801 aaaggtgtta aagagttaaa aggagcagaa cataatgtaa ttccagacag aatagaagca
    16861 gcaacttata tggttgcagc agctatgaca aaaggtgata taactgttga aaatgtttta
    16921 atggagcatt taaaaccagt tgtagctaaa ttaagagaag ctggttgtga aataacagaa
    16981 atggacaatt ctgtaagagt tgtaggtcca aaagtattaa aaccaataga tataaaaact
    17041 ttaccacatc caggatttcc tacagatgtt caagcacaat ttatggctat gcttactgta
    17101 gctaatggaa ctggtgtagt tatagaaact gtttttgaaa acagatttat gcatgttgct
    17161 gaatttaata gaatgggtgc taatataaaa atagatggaa gaagtgcggt tgttaatggt
    17221 gtagatgagc tacatggtgc agcagtaaac gctactgatt taagagcagg ggcagccctt
    17281 attttatgcg gtcttatagc agaaggagaa actcaaatag gagaaatata ccatatccaa
    17341 agaggctatg ttgatataga taagaagata acagcattag gtggtcaaat cgaaatagta
    17401 gaagattaat aattggttta tagaagaaag ttataaatta atagggttgt tcatatactt
    17461 aaatacaagg ttttaaaagc taaggaggag agtatatgaa gaacccattg gttgttttgt
    17521 taggatttgt gacttgctct gtgttagttc catcacttat aactttagtt tcttataaaa
    17581 atgtagaatt aactgaaaaa gcagagagcc cagtatctat aaacaaaact attaaaaaaa
    17641 gtgatattga agacaaaggt aataaagaag aaaaaaatac agtgaactat gaaactgtaa
    17701 ataaaaaagc accaattatc aatgtatata atcatataac agggaaaacc gaaaagatgg
    17761 acatggagaa ctatttatgt ggagtactag ctggtgagat gtcttcagag tttgatatag
    17821 aagccttaaa agctcaatct gtagcagcta gaacatatgt agtatataaa caggaacatg
    17881 gtaaatctag taaacataaa aatgcagtag tatgtactga ttataaacat tgtcaagaat
    17941 ataagagtta tgatactctt aaaaaattaa atggtgaaga atggattaag aataaatatt
    18001 ccaaaatcca agaagcagtt agaggaacta aaggtcaaat aataacttat aacgataagg
    18061 cgatacttcc tctttatttt tctacatctt caggaaaaac agaaaatagt gaagaagtat
    18121 tttctgcaaa atatccttat ttaaagtctg tggaaagtcc ttatgataag tattccccta
    18181 aatttgcgtc aacacttaaa ataagcaaca cagattttgt taaaagtctt agaagggcat
    18241 atagcactat tgtgatagat gtgaataatt taagtaaaca agtttcaata actaaaagaa
    18301 gtgatgccgg aactgtagaa aaaataaaat taggaaataa agaattgact ggaaaagaca
    18361 ttagaactgt ttttaaattg aactctgcaa actttgacat aaaatttgga gaaggataca
    18421 tagatttcgt ggtaaaagga tatggtcatg gtgttggtat gagccagtgg ggagcagaag
    18481 gaatggcaga agagggctat aaatattatg atatattatc acattactat acagatacaa
    18541 aaataaaaga tatatactaa taattgtata aaatgccaaa aacacgtcaa taatccaaat
    18601 gtagaaatat atttggaggt gcaaaatgaa gaaaaagctg ttagaaaaag atggttttta
    18661 tttatctttg tttgtatgtg tttgtttatt agcagtaggt ggagtttggt ttacaaataa
    18721 taatgtagat aaattggctt ctaataaagg aattatggaa aatgctaata aagatagtga
    18781 agaggaaata catttaattg aaaaagacaa gaaagatgct atccctactg caacggattc
    18841 aaaacaaaac ttagaaaaag ctaagtcaaa agaggaaaat aaatcgagta caacaaaatt
    18901 aaattacatt ggagataaag taataagagg atattcagaa aaagaaccta gttattctaa
    18961 aacacttgat gtttgggaaa ctcataaagg tgtagatatt agttgtacta aaggcaaaga
    19021 agtaaaatct ctattaaacg gaatagtagt agatgtattt aatgatgaag aatatgggca
    19081 atcagtcaaa ataaaaagtg acaataacat tgtagtagta tattcaaatc tagataaaaa
    19141 tgttagtgtt aagaaagaac agaaggtaac agaaggacaa tctctaggta ctgtaggtag
    19201 tacttctcaa atagaaagtg aagaaggtat acatgttcat ttagaggcgt atagtggaga
    19261 aaaatctata gacccaatga gtctaattaa gtaaaagtat aacaagttta atcatatttg
    19321 gttaagcttg ttatattttt tggtgttttt acatatatat cattaaagct attatgtgag
    19381 gggggtaatc tttgagatct catatagagg aaagggccat agtggtagca aaatatatac
    19441 ttgaaaaaaa cactacagta agacaaaccg ccaagacatt tggagtaagt aaaagtacaa
    19501 ttcataagga tgttacagaa agattgaagg aaataaatcc atcccttgcc aaagaagtta
    19561 aaaatgtgct ggacaaaaat aaatcagaga gacatattag agggggatta gcaacgaaat
    19621 taaaatatga gaaggaacat aaaaaaatgt aggttaaaaa cctacttttt ttatttaatt
    19681 aaattataaa aagaaatata ttaagtaatt ataaatagca aaataataat actctcaaaa
    19741 gatttatttt ctcttgatta aagcgagatt tttgtatatg ctttattata ggagtattaa
    19801 aataggctta taatataatc taatattata aataaaggag ttgaaaaaat ggctggagct
    19861 gatataggca tagatttagg tactgcaaat gtgcttgttt atgttagtgg taagggaatc
    19921 gttttagaag aaccgtctgt tgtggcgata gacaacagaa cagattctgt tttagctgtg
    19981 ggagaagaag ctaaaaagat gatagggaga acaccaggta atatagtcgc tataagacct
    20041 ttaagagatg gtgtaatttc agattatgat gtaactgaga agatgttaaa atcttttata
    20101 gataagatag ttgataaaaa gggatttgga agatttttta tgccaagaat tatggtatgc
    20161 gtaccaacag gtgtcacaga agtggaaaag agagcagtag aagatgctac gagacaagct
    20221 ggagctagag aagtatatat aatagaagaa ccaatagcag cagccatagg agctggtgta
    20281 gacatatcac agcctaatgg taatatgatt atagatattg gtggaggtac tgtagatata
    20341 gctgtaatat ctctaggagg agctgttgta agtgaatcta taaaggttgg tggagataga
    20401 tttgatgatg ctatagttaa atatatgaaa aaacaacaca atcttcttat aggagaaaga
    20461 acagcagaga aaatcaagtt tgaaataggt tctgcattta agagagaaga agaaaaatat
    20521 atgaagataa caggaagaaa tttaattaca ggacttccta gttctataac tataaattca
    20581 acagaaatgt tggaagcact tagagaatgt gttgagcaaa tagtagttgc tactcatgct
    20641 gttttggaaa aaacgcctcc tgagttagca gcagatatag gagatgttgg tataataatg
    20701 actggaggag gttcattatt atatggatta gataaaataa tagaacagag aacaggaata
    20761 actgttacaa tagcagatga accattatct tgtgttgcaa aaggtacagg aatagcttta
    20821 ggttcaatag acctacttga aactggcggg tctttcaaaa gaaaataatt aggagagttt
    20881 agaatgttaa atatagatga aataaaaaaa ttaataccac atagatatcc ttttttatta
    20941 gtggacaaga ttacagagct agaagttggg aaaagagctg ttggtataaa gaatgtaaca
    21001 gttaatgaac catttttcca aggtcacttt ccagaatatc ctttaatgcc aggagtatta
    21061 atagtagaag ctttagcaca agtgtgtgga gtggcaatga tgtctgtaga ggaaaacaaa
    21121 ggaaaactag gtgtatttgc aggtatagat aaagttagaa tcaagagaga agtaagacca
    21181 ggagatacat taactatgga agttgaaatg acaactctta gaagaaatat agccaaggca
    21241 gatgctaagg catatgttgg tgaagaactt gtctgtaaag gggaattaat gtttgcctta
    21301 gtagaaaaat aaaaacttga aatataaaag taaaaaggta gataatttgg agtatttaat
    21361 cagatactct aaaatatcta cctttttata taaagaattt tatcatttag tttatattta
    21421 gattaggtgg atactaataa agccaaggct attgtttcag ctaagtctaa tataaagcta
    21481 agacgtatat tattaaatga aagtttatta ttttcatcaa ttttatctac aattcctact
    21541 atagaataat ttcctatttg aggtagtttt ttgccaacac cttttccggg caggataggt
    21601 ccttctctaa tctgtatatt acctatgtta ctctgcgacc ccaagcaagc atctatagcc
    21661 aagaagttac cttgtatatg tgtatttttt atagtatcta aagactcata tatatttaag
    21721 gcgtgaatag ggttatctag tgtaccgtaa acggggtatt taaagttact gttcttaagt
    21781 attgttccaa ctaaagggcc taaagtatca cctatagccc tgtctgttcc tatgcaaaca
    21841 acaatcgtat tttcattaat tatagttttt agaactgaac ttagcatctg tataacatca
    21901 tcatcgctgt aatttacttt agctaaatac aataggaaca cccccttcaa aaaaatatat
    21961 gaataaaaag cattaaaaag acctaacttt aaatttcaga tagatagaaa attttatttg
    22021 tcttacaaaa aaaggaatag aaaaaatatg actaaaaata ttgacaaaat tttaaaaaaa
    22081 tagtaagcta gtaaagtaaa acaaaactta aatcttgatt accttatcaa gagaggctga
    22141 gggataggcc ctgtgaagcc cagcaaccac ccaaatggga aaaggtgcta attcctacaa
    22201 gacaaaagtc ttgaaagatg aggtcagtta attagtatgt aattacgatt attaacctct
    22261 tcttataaag gagaggtttt ttattttgag taaatttcta aaattaaaaa taaggtaaaa
    22321 cctataataa aaactaagaa taaataaggg ggaataaaaa tggcaagaca tttatttacg
    22381 tcagaatcag ttacagaagg acatcctgat aagatatgtg accaaatatc agattcaata
    22441 ttagatgcat tattagaaaa agacccacaa tctagagtag cttgtgaaac tacagttact
    22501 actggtttag ttttagtagc aggagaaata agtacatctg cttatgtaga cattcctaaa
    22561 ttagtaagag aaacagtaag agaaattgga tatacaagag caaaatatgg atttgattgt
    22621 gatacttgcg cagttataac ttctattgat gaacaatctg gagatatagc tatgggagtt
    22681 gatgaaggcc tagaaagtaa gactggagaa gagatagaag aagaaataga aaaagttgga
    22741 gctggagacc aaggtataat gtttggattt gcatgtaatg agactccaga attaatgcct
    22801 cttccaatat ctttggcaca caaattatca agaagactta cagaggttag aaagactggt
    22861 ctagttgatt atttaagacc agatggaaaa actcaagtta ctgttgaata tgaaggaagt
    22921 aaagctgtaa gagtacatac agttcttata tcagctcaac attgtgaaac agtatcaaat
    22981 gataaaataa gagaagattt aattaatcat gttattaaag aagttatacc agcagaatta
    23041 cttgatgaag atactaaaat ttatataaat ccaacaggaa gattcgttat aggaggacca
    23101 caaggagata caggtcttac aggaagaaaa ataataatag atacttatgg tggatattct
    23161 agacatggtg gaggagcttt ctcaggaaaa gaccctacaa aggttgatag atctgctgct
    23221 tatgcagcta gatatgttgc aaaaaatatt gttgcggctg gtcttgcaga caaatgtgag
    23281 atagaacttg catatgctat aggtatagct agaccattat ctatatttat agatactttt
    23341 gggacaggca aagtttcaga agaaaaactt gttgaattag taaataaaca ctttgattta
    23401 agaccaggag ccataataag agacttagat ttaagaaagc ctctttataa aaaggttgct
    23461 gcttatggtc actttggtag aactgatata gatttacctt gggaaagaac tgacaaggtt
    23521 gagcagttaa gaaaagatgc tttaggtgaa taatttttaa ctaaaaagct gttagaatgt
    23581 aattttacga ttactttcta acagcttttt tattatgagt taaatatttt taataaatgt
    23641 taatttatca tcatataatg aataaacagt atttaatctg gtcaatacat tttatataat
    23701 actatactaa atttatgcat attatgggat atatgttaaa ggatggttaa gaatgtgtta
    23761 actaatgtta aataattaaa aatatattga aatatcataa taatgagtct aatataatct
    23821 taataaactt acactttaaa aataatttta attgggggga gtagtgtatg tcaggcaaag
    23881 taaatattaa aacggttata agttttgctg gagcttatgt tgctactgta ataggttctg
    23941 gatttgcaac tggtcaagaa atactgcaat tttttacatt ttatggatat gctggtatta
    24001 taggtggaat aatctctatg gtactatttt catggtttgg agcagaggtt atagataagg
    24061 gaagagaatt gaaacttaag gagccaataa aaatttatca agtatattgt ggaaaatatc
    24121 ttggaacatt ttttgaatgg tttggtccat tgtttttatt tggaacatta gtaataatga
    24181 ttgcaggagc aggagctaca ttatcagagt actatggatt aaatccatat gtaggtagga
    24241 ttggtatgac aatcgtttcc ctaataactg tttcattagg tttgacaagg ctttctaaaa
    24301 tactaggtaa tataggacct ataataataa tatttacact tttagtagga gcaattagtt
    24361 tatttagcaa tattgatgct ttatcaaatg ctggaaatat ggttgatagt ttaaatatta
    24421 aaactgctac atcaaatgga tatttctcag gagtacttta tacttgttat aatgtaataa
    24481 tagtaataac attcttgaca ggaatgggag cttctgcagt aaataagaaa gatgctgtgt
    24541 ggggtggtat agtaggtgga gtagcactta tggcagcagc tataatgatg aatttggcat
    24601 tactttcaga cataggaaat atatacacaa aagagatacc agcactttac ttagctgata
    24661 agatatctcc aataataggt attttattct cagtagtact tttactagga atatacacta
    24721 cagctgtacc attgttatgg tcagtaacta accgatttgt agaagatgac caccctaaat
    24781 ttaaaattat aacaatagta gtaagtatat tagcttgtat aggaggtcta ctgccatttg
    24841 ataagttagt aggtacacta tatccatata caggttacat gggaatttta attttactat
    24901 gtatgcttta tagaagaatt actaaaactg aggggtacaa ggagaataaa tctgaaataa
    24961 gctagtactg taatatactt atgaaattta atttatactc taaatatgga gtaattaaaa
    25021 taaaatagct atttaaatat ggtacctgta tttcatggta ccatatttta tggatgcaga
    25081 ttttttttgt ttctgataaa atgtataata gttggaaatt acgttaagta taaaaatata
    25141 aaacttttct acgattatca tagttttaat atataaaatt atactaacat ttaaatggag
    25201 cgattattag tatttttagg agataaaaat ggaaaaatta gaaggaatga taagtgaaat
    25261 agtctttaaa aacgaagata atggttatac aatagctcat ctggtaaatg aaaatgatga
    25321 aattgtcgtt gtaggatgta tgcctacttt ggcaattgga gaaagcatag aagttgaagg
    25381 aaagtgggta aaccataaga tttatgggac tcaatttgaa gtgaatagtt ttatgccagt
    25441 aacaccatca tccttagaag gaatttatgt ttacttatct tctggaatga tacatggtat
    25501 tggtgaaaaa atggctaaaa gaataattga taagtttggt gtagatacgt tagaggtgat
    25561 acaaaattca ccagaaaaac tacaagaagt agaaggtata gggagtaaaa aagtaaaaca
    25621 aatagtaaaa agttatgaag aagacagaga attgagaaat ataataatac agctatctcc
    25681 ttttggaata acaccaaatt actgcctaaa aatatataaa aaatataaaa gcagtgcaat
    25741 agaagttata aataaaaacc cataccaact tgctgaagat ataagaggaa ttggatttaa
    25801 agtagctgat agtatagcaa ataaaattgg tatagataaa aattctaagg atagaatttg
    25861 tcagggaatc ttatacactt taaataaatc tttaagtaat gggcatacat atttaccaga
    25921 gcatgttcta atacaagatt cggaaaaatt attggagcta aatggagaaa taataaaaga
    25981 gtgtgtaatg atgctagttt ataatcaaaa aatacatgta gagaaagtaa acaatgaaaa
    26041 tcttatatac cttatgccat actatttagc tgaaaatgga gtatgtagtc aaatagtaaa
    26101 attatcacag tatgaatttg aagacttaaa aatagatata gataacgaga ttaatgtttt
    26161 agaagaagat aagaaaatta agttagcaga aaaacaaatt ttagcagtca aagaatctgt
    26221 taatagtggc gtattaatta taaccggtgg tccaggaact ggtaaaacaa ctaccattaa
    26281 tgctatcata gatatatttg aaaataatgg taaaagcgtg actttggcag ctccaacggg
    26341 aagagcagca aagagaatga gtgaaacttc taataaagaa gcaaaaacta tacataggtt
    26401 gctagaaatg ggattttcaa cagatgatga tttaactttt tttaaggatg aagaagaccc
    26461 aataaattca gatgtaataa ttgttgatga ggtatcaatg gtagacatta tcttaatgta
    26521 caatttactt agagctataa agttaggaac aagagtaatt ttagttggag atagtgacca
    26581 attaccttct gtaggtgctg gaaatgtact taaggatatg attaattcta atattataaa
    26641 tgtagttaaa ttaaatgaaa tatttagaca agcgcaagaa agtatgataa ttgtaaatgc
    26701 ccataagata aacaatggag aaccacttta tctaaataca aaaggtaaag atttcttttt
    26761 tataagaaaa tcaactaatg aagaaatttt aaatgaaata ataggattgg ttaatgaaag
    26821 attacctaaa ttttataaag ttgataaatt aaaagatata caagttttat cctcaatgag
    26881 aaaaggtgaa ttgggagtta ctaatctcaa tatagagtta cagaagtatc ttaacaaaaa
    26941 agaaaaattt aaagttgaag aaagtttttc taaaagatta tttagagttg gcgataaggt
    27001 aatgcaagta aaaaataatt atacaaaaaa atgggaaaca gaagaccaaa aagaaagtgg
    27061 agaaggtatc tataatggag atataggata tgtttatcac atagataaag ataaaaaaac
    27121 tatttatata ttatttgacc agactaaaat tgtatcatac ctatatgatg aattagatga
    27181 aattgaccat agtttttgta caacaataca taaaagccaa ggaagtgaat ttccagttgt
    27241 agtattgcca atagcttggg ctcctcctat gttacttagc cgtaatttat tatatacagc
    27301 agtaacaaga gcaaaaaaat tagttgtatt agttggagat gtgaaatatc ttgaatatat
    27361 gataaaaaat aatagagtta atcaaaggta ttcaaattta ggatataagc taaataaatt
    27421 taagcaagaa gggctgttga tagaataaat atgaaaatat gcaatgataa ttttattaag
    27481 aattttaaaa atatgattaa taaatgtttg gattttatat atcctgagaa tataagttgc
    27541 ataatttgtg ataaatccat aaagaaaaca aatacttatt caatatgtaa aagttgtttt
    27601 aaagaaatga attttataca agatggttgc atgaagtgtg gaaaaccaat aatacgtcat
    27661 tcaattgaaa aagaatttat tgaagaatgt agttattgtt ttaataaaga cttttatttt
    27721 gataaatcaa tatcttgtat tgaatacaat gatgtgacaa aaaagatagt gctagggctg
    27781 aagtataacc aaaaaacctt tatggctaag tatatagctc aaataatgaa agaaaaatta
    27841 tatttagaaa acataaaatt tgattatata ttatttgttc cacttcataa aaaaaggcta
    27901 aataaaagag gttttaatca ggcacaaaaa atagcattta atttaagtaa aatagtaaat
    27961 attccattat tggattgtat aagtagaaaa aaatatacaa gaatgctata taaattgaat
    28021 aagaaagaac gaaaagaaga attaaaaaat gtatttgttg ttaaagacaa tgttaaatta
    28081 ataaataata aaaatatact tttaatagat gatatattta ctactggtct aacaacaaac
    28141 gaaatttcta aacttttaaa gttatcgggt gctaataagg tttttgtatt gactctactt
    28201 acaaaagcaa atgataatta tattatggaa taatcttata tattgacaat tttaatttaa
    28261 aatattaaaa tgaataagtg atatttaggt ctgtggttga aagtccaagc cagtcgcagg
    28321 caaagggatc cacgtaagtt aacactaaaa attgggttaa tgatcatggt gcggcttaga
    28381 agtaagacct accgataata aaatcgagag agagtagtag tgcggcgaac ccaagcgaac
    28441 cctccagtag gcgagtgtgg ggtcaaaaac caggtcagct aaatatcgta ttaaaaggga
    28501 gctagcatac ctttatgtat agctcttttt tatatataat aaatttaggt tgagtcataa
    28561 agaggtgata tgtttgaata aaagacagga aaacttatta acaacgttgc tattaaaaga
    28621 agagtttata aatgttaata agattgccaa tgattttgag tgttcagaaa ggacaataag
    28681 aaatgattgc aaatatattg atgaatggtt atctactttt tgtaatgctt atatcaaaag
    28741 aaaacctaat attggaatta aatttagtgg agatggaaca gacaaaatac tggtaaatca
    28801 gaagttgcga ggtgaagtta aaagagtaaa tagtgagctt cataagcaag ttgagatatt
    28861 aaatttacta ctttgtaata ataaaaaagt tactttaaat gatttatcta ataagttgta
    28921 cataaataaa aatattgtca gagaagaaat aagtaaatta tcagtggcat taaataatta
    28981 taatttaaat gtctcgacta aaaaaggcag tggtatatat attggagggg atgagaaaga
    29041 cataagaata atgttagtat cattcttatt taaatacata gataaataca gattaaacat
    29101 agatgaaata gagtacttcc atatagtaga tataataatt gtaaaaaaca taatgtttac
    29161 tatagaggag gatatggata taagacttac agatatatca ttcaagcaat tgatgctatt
    29221 tttacttata aatataatac gaataaggct aggaaattct ttaaaagtat caaatactaa
    29281 gaaagagctt aagctaaaaa tgctgatatt agatatagaa gatgaattga aatctaattt
    29341 atccatagta ttaaattatg aagaaaaatt gtttatagag tctttaatat tgggtatgaa
    29401 caaacaaata aatagtagta atattaagaa atctttatat ctaaatgaag aattagcaag
    29461 ttatacaaaa aaaattataa atttagtatc agaagaatcc ggaataaatt ttaataatga
    29521 tatattactt tatgaacaat taatatgtca tttgaatgtt acaatgcacc aaatgaaaag
    29581 caatgtttac ttagaaaatc cacttttaga taacataaaa actaaatttt gttttttatt
    29641 tacagtaata tcaagttcaa tagaaattga attaaaagga aaacaaataa cagaagatga
    29701 aattggttat cttacacttc actttcaaac ctctctagaa agaaaataca gcaaaaggga
    29761 atcaaataaa aaggcaagta ttgtatgtcc atttagtttt ggagtaagca tgctattaaa
    29821 agtaaagata gaaaagagat ttgataacat aaaaattatt gaaacattaa gagaggaaga
    29881 tttaaaaaga gataacttca ataagactat agattttata attgcatttc aagagtatga
    29941 aaatatccaa aaaccaattt ttataacaac accattattt actgatgagg atgagaataa
    30001 acttaaagag ttcacaaaca aaataaaaga aaaagacact tcatataaac ttatgaacag
    30061 attaatgatg tctgattatt taataagaga gttagaacat gatgatattt atgaagctat
    30121 aacatattta actaatttat taattgaaaa acattatgca gaaaaagagt atttacaatc
    30181 tattataaca agagaaaagt catatccaac caatgttgga aaaggaatat tattccctca
    30241 tggagatatg aaatatataa aacaatctgt tttatgtttt gcaaggttaa aaacaccaat
    30301 aagaattaga aatggaaagg atataaagat tatacttttg ttagcatata aaaaagatga
    30361 tgatagaaaa gtatttagag aattatttaa agaaatatct aatttaacag aggatgaaaa
    30421 tatgctagat attctaagta aatgtgacat tgaaaaagta aaagatattc ttatataaaa
    30481 ttcatagtga actatggata agcacaaaat tttccatgtg atggaaaaag ttgtgcatgt
    30541 ttttttgttt ttataagata ttatatgttt gtaaggtata aataatctaa aagtagagaa
    30601 gcaacttagg aggaaaaaat ggaagaaata gttttaaaga ttataattca tgctggaaat
    30661 gcaaaatcta tgctttatga agcacttgac tatgcaaagg aaaacgattt taaaaaagca
    30721 gatgaattaa ttgaaaatgc aaatgaagaa attttaaaag cacataaagt acaaactgaa
    30781 cttatacaaa aggaagctgg gggagataaa tcagatatat ctattttatt gattcactct
    30841 caagaccatt tgatgacttg tatgagtgag agaaacctta taaaagaaat gattatgtta
    30901 agaaaagaaa ttcaaaaatt aaaataaaaa aattaagggg gatttaaaaa atgaaaagaa
    30961 aagtatattt attttgtagc tttggtatgt caacaagttt acttgcagat aaaatgcaga
    31021 aggtagcaga tgaacacaat ttaccaatag aagtagaggc atttccgata gcagaaattg
    31081 acaaaatagt tgaagaaaaa aatccagatt gtatattact aggaccacaa gttaaataca
    31141 tgctaaagga attaaaacca aagtttgaag ctcaaggaaa acttatagat ataatcaatg
    31201 aagttgatta tggaactatg aatggggaaa aagtattaaa actcgctata aagctaataa
    31261 aatccaaaca agcgtagaat tatagtaaaa aataatagga aatgagggta gatagatgaa
    31321 tagattggaa agaatactta tgccattggc tgaaaaaata ggtaaaaata aattattaat
    31381 tgcaattaga gatggttttt taatatcatc accattatta ataataggtt cattattttt
    31441 attagtagca aattttccta taaaaggatg gaatgaattt gtaggtcaaa tttttggttc
    31501 agattgggct gtaaaattgc aacaaccaac tgtagcaaca tttgaagtaa tgactctact
    31561 tgcagtttta ggaattggat attcttatgc aaagcaaata gatgtagacc caatagcatc
    31621 agcagcagtt gcaatagttg ccttttttat aataacacca tttgtaatac catatacacc
    31681 agaaggaaca gaatcagtct acatggtaac aggtatacca ctaggttgga tgggctctaa
    31741 gggaatgttt gtaggtatga taacagcaat aacctctgta aaattatttg ctgcagttgt
    31801 aaaaaaagga tggacattga aaatgccaga tggagttcct ccaaccgtat caaaatcatt
    31861 tgcagcatta ataccaagtg caatagttat ggtagttttc tttttagtta agatagcttt
    31921 tgaagctaca ccatatggaa gtatacatga atttatattt aagtttttac aaatgccatt
    31981 attaaaatta ggaaatagtt taggtgctat ggcaatcgct tatatattct tacatttatt
    32041 ctggttcttt ggaataaatg gaagtagtgt tgttggggct gtgtataatc caatattaaa
    32101 aattctatca gctgaaaatt tagctgcttt tcaagctggt tcaaaaatac caaacataat
    32161 aacaggtcaa ttccaagata tgtttgcgac atttggtgga gcaggtagta cactatcatt
    32221 agtcatagct atgttattaa tatgtaaaag taaacgtata aagtcaatgg gaaaactatc
    32281 aattttacca ggaatatttg gtataaatga gccattgata tttggtctac caataatgct
    32341 taacccaata ctattgatac catttgcaat agttccaaca ataaatataa taatagctta
    32401 tttctgtatg tcagcaggat tagtgcctct tacaaatgga gttcagttac cgtggacaac
    32461 tccaattata ttctctggtt ttttaacaac aggatggcaa ggtgcagtct tacaagtagt
    32521 gcttcttata ctaggaatat ttatgtatat accatttata aaaatgctag ataagcaata
    32581 cttaagagaa gaaaatgcaa ctgttgaaga ggaagatgat gatatatctt ttgatgattt
    32641 agtattatag gaggaagatg atgaagaaaa tagttatgat attggatcaa atacaagctg
    32701 gagctggagg aaaagagaaa agtaatatac ctcctgctgg aaaatcaagc ccattaggac
    32761 caggtataat gatggagcaa tttttaaatg aatctaaggt aatagctacc ttattttgtg
    32821 gggatgaatt ttttgtaaat aatgaagaag aagtaactag taagatgata gctatggtaa
    32881 aaaaattaaa ccctgatgta gtaatctgtg gaccaagctt taattacgaa aacttttcta
    32941 agatgtcagc aatactgtct aaaaatataa atgataaaac agatatacca gcttttgcag
    33001 ctatgtcaga agaaaatata gatgtgataa acgaatataa aaatgacata tgtatagtta
    33061 agactccaaa aaaaggtgga attggtctta atgattcatt aaataacata tgtaaattgg
    33121 caaaagcaat agcaaataaa gaagatataa ctttgatgaa agaagaattt tgttattaat
    33181 aagagtatta attattagta agtttaaata ttatactatt tataataagc atacattagt
    33241 aaaatctata taatttaagg aggggaattt taacatgtgg ggaatgatag cgacatggag
    33301 aatggcccta gaaggaataa ctgaggcatc agaacattta gaaaaaggca tggaagcagg
    33361 tgatgctgta gagcttgctg taagaagagt tgaagactat ccattctata aatcggttgg
    33421 atatggtgga cttccaaatg aaaattgtga ggtagaactt gacgctgcat ttatggatgg
    33481 taacacatta gatattggag cagttgctgg tataagagat tattctaatc ctgtcagcat
    33541 agctaggaag ttaagttatg agaaggtaaa taatttttta gttggaatag gtgctgaaga
    33601 ttatgctcat aaaaatggat ttgaaagaaa aaatatgttg actgatagag cgaagttaca
    33661 ttataaaaag agaaaaaaag agactttaga caaaggatta agtccatatg ctggtcatga
    33721 tactgttggt atgatatctt tagacaaaac tggaaaaatg tgtgcagcta catcaacaag
    33781 tggattgttt atgaagaaaa gaggtagaat aggagattct gcactatctg gttcaggatt
    33841 ctatgttgat agtagtatag gaggagctac agctactggt ttgggagaag atttaatgaa
    33901 gggatgtata tcttatgaga tagttagact tatgaaagaa gggaagcatc cacaagaagc
    33961 atgtgatatt gcagtaaatg aacttgataa acagcttatc aaaagaaggg gaaaagctgg
    34021 tgatttatca ttagttgcca tgaataataa aggtgaatgg ggagtagcta caaatatagc
    34081 tgattttact ttttcagtag taactaaaga agaatctcct actgtatata tttcaaatag
    34141 agtagatgga aagactatac ataaggttat atcaaatgag tggatggaag catatgaggc
    34201 tagaataaaa gcaccaatag aagtaagtga gtaaagtagt ggttacataa ttaaaataga
    34261 gttttataat aaggagattt ttatgaatat cataagtaat aaaatagatg agttaaaaga
    34321 agatttatta agtgatatta tagatattgt aaaaatacct agtgtcaaag gggagtcaga
    34381 aaatggcttc ccttttggcg aaaaagtagg ggaggcactt aataaagcat tagaaatatc
    34441 agaaaaattg ggatttaagg ttagaaactt ggataattat attggttatg cagagcatgg
    34501 agatagtgat gattatgttt gtgtaattgg acatgtagat gttgtacatg aaggagatgg
    34561 ttggaagcat cagccttata agggagaaga aactaatgga agaatttatg gacgaggagt
    34621 actagacaat aaaggaccta taatgtctgc cttatatgga ttatatgcta ttaaagaatt
    34681 aaatttaaaa ttagataaga gagttagaat aatatttggt acaaatgaag aaagtggatt
    34741 tgaagatata ccatattatt tagaaaaaga aaaagcacct attatgggat ttacaccaga
    34801 ttgtaaatat cctgttgtat atggtgaaaa aggcatggca aagattagaa taaaaagtaa
    34861 aatcaattat gaagaagatg tatacttagg ttttatagaa aatatgtctg aaaatgtgct
    34921 tgttacatat aaagaactga atatagaaaa tagtgatact atattagata taaaggtaaa
    34981 atacgatttt agttataaat tgaaagatgt attggatgaa ataaaagcta gttttccaaa
    35041 tagtatcgat atagaggtaa tttctaattt taatcctgta tattttgata aagagagtaa
    35101 tcttgttaaa aagcttcagc tagcttatga aagggttaca tcattagatg gaactccagt
    35161 aacaactaat ggaggtacgt atgcaaaagt aatgccaaat atagttccgt ttggaccttc
    35221 atttccagga caaaaaggaa tagctcataa tcctgatgaa tacatggata tagaagatat
    35281 aatattaaat gcaaagatat ttgcaaatgc aatatatgaa ttagcaaagg agtagttatg
    35341 ttagaaataa taggaatgtc agtagaagac gctaaaatta tagaagattg tggtgcagat
    35401 agaatagagt tagttagtgc tttaactgaa ggtggattga caccaagttt tggtcttata
    35461 gaaagtgttg ttaacagtgt aaagatacct gtgaacgtaa tgataagaca tcatgcgaaa
    35521 agttttgtat acagtaaaga agatataagt ataatgcaaa aagatataag tgtggttaaa
    35581 gagattggtg ctaatggagt tgtatttggt gtattggata aaaacaatag tatagatgag
    35641 aaaaatctaa atgttttatt gaaatgttgt gataatcttg atgttacatt tcatagagca
    35701 atagatgaat caaatacaat agatagtgtt aaaattttga aggattatga taaaattacg
    35761 aatatattaa catcaggagg gaaaggaagt atagttgata acatacaaat gataaagaat
    35821 atgatgctta gttctaatca cataaaaatt cttttaggtg gaggtcttaa ttttaataat
    35881 atagaaaaga taaaggaatt aactaaggca agtaattttc attttggtac agcgattaga
    35941 ataaataaca gtccttttga agatatagat agacaaaagc ttaaacaact cgtaaatata
    36001 atttcaagat agtatatgaa tataaaatac ttagtttaaa taaagagagt aagttaatta
    36061 taatttgggt atatgaatgt taataggtat taaatatgaa attattgttg agtatgcttt
    36121 ataactttta agtagtttta aatataaata atgaaagaaa agatagatta tggaggtttt
    36181 agagggttta tatttattaa aaaattataa aggtatattg gcatataata cacataattt
    36241 cctaaaatta ccatcttatg acatgaattc acatattaat aaacaattaa tggagatttt
    36301 tgtggataga aataggagta aaaatagctt taaataaaca aaaacaagag ttattaacag
    36361 ttttatccac attatccaca ggtgtttgta ataatattaa tccacataag tatcaatgta
    36421 actaaaaatg ataaataatt ctatcaattg aatagaaaaa atcataaaag taaataataa
    36481 aaagaggatt atgagagttc gtgtagaata tattattaaa gtataaattt tcttatactg
    36541 gcataattga agggagatgt atttatgaac ataattatat ctggaaaaca aatcgaatta
    36601 actgatgcaa taaaaaataa aatcgaaact aaactaagta agttagataa ttacattcat
    36661 ccagacactg atgtaaaagt tacagtaagt gcgagaaaag caagacaaaa aatagaagta
    36721 acaatagcta ctgtaaatgg tcctataatt agagcagaag atatgcagga aaatttatac
    36781 acagctatag atgttgtata tgataaatta agtaaacaat taaaaaaata taaaaagaga
    36841 ttacaagata aacataaaga caacaagagt ataagattcc aagatgcaga gataaatcta
    36901 gctgatgaac aagaggatta tggggatgaa aatcaagaat tagtaattca aagacgtaag
    36961 aaatttagtg ttaagccaat gagtgaagaa gaagctgtat tacagatgga attaatagga
    37021 catgattttt atatgttcaa gaacatagat tcagatgaaa tagcagtagt ttataagcgt
    37081 aataatggtg gatatggtat aatagaacat gaataaagca atttagattt aatcatatta
    37141 gctatcgata aataaattat cgatagcttt ttaaattaat attaaaagta tagtttttat
    37201 ggtaaaatga ataatagtac aataaagaac gaggagagat gtaaaatatg agctttatgg
    37261 ataatttatt caacatggca gataaaaaag aattaaaaaa gttcaataaa actgttgata
    37321 taatagattc attagaacct aaatttgaat ctatggcaga ctcggaactt aaaaatatga
    37381 caaatatatt taaagaaaga ttagcaaatg gtgaaagtat agatgatata cttccagaag
    37441 cttttgcagt agttagagaa gtttctaaga gagtgttagg acttagacac tacagagttc
    37501 aaatgattgg aggtatagta ctacatcaag gaagaatagc agaaatgaaa acaggtgaag
    37561 gtaaaacttt ggttgcaact gctcctgttt atttgaatgc gcttactgga aaaggtgtac
    37621 atgttgtaac agtaaatgat taccttgcaa aacgtgatag agaccaaatg gcaaaaatct
    37681 atgagttttt aggaatgagt gtaggcgtta ttatacacgg tcaaaatcca aaagttagaa
    37741 aagaacaata tgattgtgat ataacttatg gtacaaataa tgaatatgga tttgactatc
    37801 taaaggacaa tatggtaata cacaaagagc aaagagttca aagaggatta aattatgcta
    37861 tagtggatga ggttgactct atactaatag atgaggctag aacaccactt atcatatctg
    37921 gacctggaga caaatcaact cacttatatt cagatgcaaa cacatttgtt ttaaccttaa
    37981 aaccagatga ttatgaatta gaagaaaaag ataaggctgt atctctaaca gcatcgggta
    38041 tacaaaaagc agaagtgtat tttaatgttg ataatataac agatatatct catacagaat
    38101 tataccatca tataaatcaa gctttaagag cacatgtaat aatgaagaaa gatgttgatt
    38161 atgttgctaa agatggagaa atagtaatag tagatgagtt cacaggtaga cttatgtttg
    38221 gtagaagata ttcagaaggt ttacatcaag caatagaggc taaagaaggt cttaaaatac
    38281 aaagagaatc taaaacacta gctactgtaa cattccaaaa ttattttaga atgtacaaaa
    38341 aactttctgg tatgactggt acagcaaaaa cagaagaaga agagtttaaa gcaatctaca
    38401 aaatggatgt tttccaagta cctactaata agcttatgat aagggaagac ttaccagatt
    38461 gtgtatacaa aagtgaaata ggtaaattta atgctgtagc acaagaaata attgaaaggc
    38521 acaaagtcaa tcaaccaata cttgtaggta cagtatcaat agaaaaatca gaattattat
    38581 cacaaatact aaagaaaaag ggcataaagc acgaggtatt aaatgctaag catcatgata
    38641 aagaggcaga aataatagca caagctggta gacttggagc tgttacaata gctactaata
    38701 tggctggtcg tggtacagat atagttctag gtggaaatcc agacttttta actaagagag
    38761 aaatgagaag aaatggattt aaagaagaaa tagtaaatag agtagatact ccaatagaag
    38821 gtatacctgt aaaaggtaat gaaattctat ttgaagctag agaagagtat gaaaaactat
    38881 ttgaaaagtt taagcaacaa acacaagaag aacaaaaaca agttgtagaa gcaggtggac
    38941 ttgctataat aggtacagaa agacacgaat ctcgaagaat agataaccag ttgagaggtc
    39001 gtgctggacg tcaaggtgac ccagggagtt caagattcta tataggtctt gatgatgacc
    39061 ttatgagatt gtttggtagt gatagaatat ctggtattgt agataaaata ggtcttgaag
    39121 aagatatgcc gatagaacat agaatattat ctaagtctat tgaaggagca caaaagaaag
    39181 tcgaaggtaa aaactttgga ataagaaagc atgtacttca atatgatgat gttatgaata
    39241 aacaaagaga aataatatat gcagagagaa aacgtgtatt agaaggtgaa gatttacaag
    39301 aacaaataca atcaatgact cattctataa tagaagaagc agttactctt tatacacaag
    39361 ataaaggatt tgatgaagaa ggatttaaag aacatatgta taatctgttc ttgccaaaag
    39421 gtagcataga aataccagaa atagaaaaac ttaatccagt tgaaataaca gaaaaagttt
    39481 atgaaatagc tatgaagata tatacttcaa aggaagaaca agttggctat gagagaatga
    39541 gagaagttga gagagtaatc ttacttcaag ctgttgataa ccattggata gaccatatag
    39601 atgctatgga ccaattacgc caaggtattg gtcttcgtgc tgtaggtcag caagacccag
    39661 ttattgctta taaaatggaa ggttttgata tgtttgatga aatgaataaa catatcaaag
    39721 aagatactgt aagatattta tttaatataa caatagaaac tccagtggaa agaaaagcag
    39781 ttgtagatgt tgaaaatcta tcttctccat cagatggaac acttccaaca agtaaaactg
    39841 ttaaaaaaga tgaaaaggtt ggaagaaatg acttatgccc ttgtggaagt ggtaaaaaat
    39901 ataaaaactg ttgtggaaga taatctcgga ggattaaatg ttaaagttac aagagtatag
    39961 acatagtatg gagcagatgg cttcaaattt agaagaattg agggtttctc tttgacatag
    40021 cttcattgat gaaaaatata caaattaatg aagaaaagat gaataaacaa gacttttgga
    40081 atgataatga agtcgctcaa agagtacttc aagaaaataa gtctttaaaa gagacattag
    40141 aagaatatga aagcttaaaa agcttactag aagatataga agtattaatt gaaataggtt
    40201 tagaagaaga tgatgattct gtagaaagag atatagaaaa gtccatagaa tcaatggaag
    40261 aaaaattatc tgaaatgaaa ataaagactc ttctaaatgg agagtatgat aaaaataatg
    40321 ctatattatc tataaatgcg ggtactggtg gcttagatgc tcaagattgg gctcaaatgc
    40381 ttttgagaat gtatataaga tggtcagaaa gtaagggtta taaagttaag ctcttagata
    40441 taatatcaga ccctgaagcg gggataaaaa cagctacaat tcttgtagaa ggaacaaatg
    40501 cttatgggta tttaaagagt gaaaaaggag ttcatagatt agtgagaata tctccatttg
    40561 acccatcagg aaaaagacat acttcatttg catctataga tgtaacacca gagttagatg
    40621 aaaatataga agtagaaatt aatccatcag atttaaagat agacacatat agagcttctg
    40681 gagctggagg gcaacatgta aatacaactg attcagcagt tagaataaca catatcccta
    40741 ctggtgtagt agttcagtgc caaaatgaaa ggtctcagca tctaaataag gacagagcta
    40801 tgagactttt aatggcaaaa ttgatagaat taaaagagtt agaacaaaag gaaaaaatag
    40861 aagatataca aggcaaatac tcacaaataa cttggggtag tcaaattagg tcatatgttt
    40921 ttcaaccata taaattggtt aaagaccata gaacaaatgc tgagtttgga aatgtagata
    40981 gtgtaatgaa tgggaatata gacttgttta ttaatgaata cttaaaaatg aataaaatag
    41041 tttaataaat atgcactgta attaagatat atgtataaga aggttagtgc ttaatatgca
    41101 cttacagcac ttaggctgag gacgataacc ctcagccttc gttctgtttt gttatactta
    41161 tgaaaaacta acattaggag tgaataaatt ggatataaat caaattttaa aaaaagagtt
    41221 taatcttaga gatgaacaaa taaataatac attaaaactt attgatgaag gaaatacaat
    41281 accatttata gctagatata gaaaagaaat gactggcgaa atgagtgatg ttacactaag
    41341 agaattttat gaaaagttaa tgtatttgag aaatcttcaa agtagaaaag atgatgtagt
    41401 tagactaata gatgaacaag gtaaattaac agatgaaata acacaaaata tagaaaaagc
    41461 aaaaacacta caagaagtag aagatatata tgctccatat aaacaaaaga aaagaactag
    41521 agcaacaata gcaaaagaaa aaggattaga aaaattagca ttgtcaatac tagagaataa
    41581 tttagataat atagaaatag aggcaaagaa ttatcttgat gaagagaaag aagttctttc
    41641 tattgaagat gctctaaaag gtgctagaga tataattgca gaactagtct cagatgatgc
    41701 taagattaga aagtatataa gagaattagc tttaagagaa ggtatgatag ttagtaaaag
    41761 tgccactgat gagaaatcgg tttatgacat gtattatgat tatagtgaag cagtaaaaag
    41821 tatgcctcca catagagttt tggcaataaa cagaggagaa aaagaaaact tcctaaaggt
    41881 taagttagaa ataaataatg ataaagtttt aaattacata ataaacgaat atgtaaatga
    41941 taaaaatttc aaaaataaag aagaaatcgt aagttcaata gaagattctt ataaaagatt
    42001 aattttccca tcaatagaaa gagaaataag aaatcattta acagaaatag ctcaagaaag
    42061 agctataagt gtatttggaa agaatgttaa aagtttattg cttcaaccac cagttaaaga
    42121 caaagtagtt atgggatttg accctgcatt tagaactgga tgtaaaatag ctgtagtaga
    42181 taaaaatggt aaactactag attatacaac agtatatcca acagaccctc aaaatgatgt
    42241 agagggagca aagaaagttt taaaaggatt gattgaaaaa tacgatatag atataatttc
    42301 aattggaaat ggtactgcat ctagagaatc agaaactttt gtatcagaaa tgataaaaga
    42361 aatagataac gaagtgcagt atgtgatagt tagtgaagca ggagcatctg tttattcagc
    42421 ctctgaactt gcaaatgagg aacatccaga tataaatgta tcaataagag gagctatatc
    42481 tatagctaga agattacaag acccattagc agagcttgta aaaatagacc ctaaaagtat
    42541 tggcgttggt caatatcagc atgatttaaa caaaaagaga ttagaagaag tactagatgg
    42601 agttgttgaa gattctgtaa atagtgttgg agtagattta aatactgcat catactccct
    42661 actagaacat gttgcaggaa taagtaaaac tatagcaaaa aatataatag catatagaga
    42721 agaaaatgga gattttacgt caagagcaca attgaaaaaa gtaaagagac taggaccaca
    42781 agcatttact caatgtgctg gatttatgag aatcttagag ggtaaaaatc cactagataa
    42841 tacaggtgtg catcctgaaa gttatgatat atgtaaaaaa atgattgaga taattggata
    42901 ttctttagat gatgtaaaga ataagaatat atgtgaaata gatgaaaaaa taaaagaaat
    42961 tggcttgaga gaattaagtg aaaaactgga agtagggcaa gttacgttaa aagatatcat
    43021 aactgaaatc aaaaaaccag gaagagaccc aagagaagaa ggtataaaac ctatacttag
    43081 aactgatgtc ttaaaaattg aagatataca agagggtatg actttaaaag gtacaataag
    43141 aaatgttgta gattttggtg catttgtaga tattggaata aaaaatgacg gattagttca
    43201 taaatcagaa atgagtaata gttttgtgaa agaccctatg agtattgtta cagtaggtga
    43261 tatagtagat gtaaaagtca ttggaattga cttaaacaaa aaaagagttg ccttatcaat
    43321 gaaaaaatag ataagtttat gtatggttat agtacttaaa ttctatgttt gtaaatgagg
    43381 ttactcgagt gtataaatgt taaaaatttg taaaagtata cagtaagaaa atatattaaa
    43441 ataagaacga tagtcttgaa aatcaggagg aaaatgaatg atttttataa aaaatggtaa
    43501 aataaatacc ataacaaatg ggataattca tggagatata cttatagatg aaggaaaaat
    43561 aatagaaatt ggagaagatt tgatagctcc tttagatgta gaggtaatag atgctagtaa
    43621 taaacttgtg tttcctggat ttatagatgc tcatactcat ttaggtttgt gggaagatgg
    43681 tatagggttt gaaggagcag atggaaatga agagactgac ccaataacac cacaattaaa
    43741 tcccatagat ggaattaatc caatggatag aacttttaaa gaagcttttg aaggtggaat
    43801 aacttctgtt tgtacaaccc ctggaagtgc aaatgttatg ggtggtcaat gtatagctat
    43861 aaagacatgt ggaaaaagaa ttgataaaat ggtaataaaa aatccagttg catctaaaat
    43921 cgcttttgga gaaaatccaa agagctgtta tggtcaggat gataaatctc ctcaaactag
    43981 aatggctata gctgctttac ttagagaaaa cctaaaaaaa gcagaagaat atttagagga
    44041 tattgatatg tatgagagtc atgatgatga agactgtgaa aaacctgaat atgatataaa
    44101 aatggaaagt ttaataccag ttttaagaag agaaattcct tttaaggcac atgctcatag
    44161 agcagatgat atgtttacag caataagaat tgcaaaagaa tttaatttaa agcttacatt
    44221 agaccattgt acagaaggtc atcttattgt agatgaatta gtggaagaag ggtttcctgt
    44281 aatagtaggg ccatctttgt cagaaaggtc taaatttgag cttagaaatt tgacatttaa
    44341 tacagcagga atactttcaa atgcaggtct tgatgtatgt ataatgacag accatccagt
    44401 aataccagtt caatatcttc caatatgtgc aggtattgca gtcaaacatg gtatgaaaga
    44461 agaaaaagca atagaatcaa ttacgataaa tccagctaag actttaggaa tagaagatag
    44521 agtaggctct atagaagttg gtaaagatgc agacctagta atttgggata attctccact
    44581 agagattcaa agtaatgtac tttacactat aataaatggc aaagtagtgt atgagaaaaa
    44641 ataacttgac aattattaaa aaattaacta atataagagt gtaaggaaaa ttaaaaaatc
    44701 aaagaagatc ttcggggcag ggcgaaattc cctaccggcg gtatagcccg cgaactactt
    44761 aagtggttga tttggtgaga ttccaaagcc gacagtaaag tctggatgag agaagatatt
    44821 tatggaagat aaataagttt attttagttt gtttaaacta tatccataaa aactcgaaga
    44881 tagggtcttc gagttttttg tttttcctaa aaaatataaa catattgatt agggggaaat
    44941 aattatgcaa aacacagcca aaagaggcaa tatgatttca acaagaactt tagtagtaat
    45001 gtcaatttta tctgcaatat cttatttatt aatgtttatt caagcaccaa taccaggtat
    45061 atttccagat ttcttaaaaa ttgacttatc agatattcca gcaatattcg gaggaatgtc
    45121 tctaggacca tttgttggat ttggaatagt tgtagtaaaa aatgtgttac aagcaataac
    45181 agcaacaaca actggaggaa taggagaatt tgcaaatata ttaattggtg gttcttatgt
    45241 aatgatactt tgttattcat ataagagaag tggagattta aaaggtgtat tgattggttt
    45301 tgtattagga acaatatcta tgactataat tggttcatta gtaaattatt ttgtaatgtt
    45361 gccactttat ggtcaactta tgggactaga tgctataata ggtttaggtt cagctataaa
    45421 tcctcaagtt catgatttat tcacttttgt aatttggatg atagctccat ttaatgtgct
    45481 gaaggctgtt atactatctt tagtaacatt gccactttat aaaaaaatgg gaaatgtaat
    45541 aaagaaataa ttagacaaga aaatataaaa ttaaatgtat tgaatagcca taatttattt
    45601 ataacttcag tcctatattg tgattgatat attaaatata ttatggcttt attaatttat
    45661 caaaaaatgt atataaaata agaaatattt agacaaattt aaattttaaa ataaaggaag
    45721 aattaaaacg attaactatt aaaatatg
//
LOCUS       DAMJVI010000019        45457 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.019, whole genome shotgun sequence.
ACCESSION   DAMJVI010000019 DAMJVI010000000
VERSION     DAMJVI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 45457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.019"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            57..866
                     /locus_tag="PC545_001168"
     CDS             57..866
                     /locus_tag="PC545_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2455946.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELDLIDKKIAEFISHKEN
                     IIKRLSSIESVIQNTKIDEIEVVYIEKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKIYMEKVFKFIEENNYKIIADPIEIYKIDIHETGIVEEFVTEIQVPITK"
     gene            1000..1239
                     /locus_tag="PC545_001169"
     CDS             1000..1239
                     /locus_tag="PC545_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455947.1"
                     /translation="MKCSNCGSVNIEKGILTTGGIYAETIGLKYRGDGKLDKVINKTF
                     PVQSTIYSDLCLDCGEIVRTYIKDNTDRNWCKDKK"
     gene            complement(1427..1603)
                     /locus_tag="PC545_001170"
     CDS             complement(1427..1603)
                     /locus_tag="PC545_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455948.1"
                     /translation="MFRDEMDKCTHMLTAYISSLYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(1657..1753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2108..2782)
                     /locus_tag="PC545_001171"
     CDS             complement(2108..2782)
                     /locus_tag="PC545_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2455949.1"
                     /translation="MNHHILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     STDSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            complement(3002..3556)
                     /locus_tag="PC545_001172"
     CDS             complement(3002..3556)
                     /locus_tag="PC545_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2455950.1"
                     /translation="MSIALILIDIQNDYFKNGKCELFQSEETAENAKKILLFFRKNNL
                     PVIHIQHISTDKTASFFLPNTHGSEINKIVFPLDNEEIITKHTPDSFFETDLQSILEK
                     KNITNLVICGMMSHMCIDTSVRTAKKLRYDITLISDACTTKNLSWNNIEINANTVHQV
                     FMASLQNSFADVKNTDKFLSNYHK"
     gene            complement(3634..3999)
                     /locus_tag="PC545_001173"
     CDS             complement(3634..3999)
                     /locus_tag="PC545_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2455951.1"
                     /translation="MKIRAEYTCLLEIVHDIIKGKWKTILIFELRQSNKTFSELQHNI
                     YGINQKMLIQQLKELRDFGIIDKISSAGYPLHVEYFLTNRGKKMLKAVEIMQEIGIEY
                     MLEHGQQEILDNKGICYNK"
     gene            4464..5249
                     /locus_tag="PC545_001174"
     CDS             4464..5249
                     /locus_tag="PC545_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2455952.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVEDGNITSIILKNKSTLIIYSFKNNFNIGEMKNHIKDLDVESISGKKCVIDRLISK
                     YKDFYEKLDNKFCVLKEIKEIDFSNMKEYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKVHLKEGNVRAYFIRNDDTMISTVSTGMETSFLAMVVSVSTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDVAGKIYHNIGYKEINEWTILFK"
     gene            complement(5346..6170)
                     /gene="pdaA"
                     /locus_tag="PC545_001175"
     CDS             complement(5346..6170)
                     /gene="pdaA"
                     /locus_tag="PC545_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2455953.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYGAYYVGNPNEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPELIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEKEGYKLKTLDYLNNKK"
     gene            6391..9510
                     /locus_tag="PC545_001176"
     CDS             6391..9510
                     /locus_tag="PC545_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2455954.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPVEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FLEYHMPKLKNKFIDLIELAVILEPYHKDYSLEYLKNTLTNCNSKVENRALSDAIDII
                     NIVNCLLVKFNNYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINIKK
                     EYNIFDSKEEKKKERETLKILNEEEKNYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLK
                     RLVEGGKYGDIEEINYLVFDNFKEISTHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIGREDKQKIARSINLIIEEIDSIL
                     KFGNCNEYNNVSNYNLRWELNLQIDEIVGKLKKDGIDTEISYRAYSEKIKLSCEKIIK
                     NLVSIIIIIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISKITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYGIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRRHLNKYRSLILKSVIFDTIKA
                     LNVDAKMDNNDVDKDIIKKDINENIRQRAVKGEVYHIDIIKKDMKVKYFDRNYLINLD
                     IFMREEDKN"
     gene            9528..10127
                     /locus_tag="PC545_001177"
     CDS             9528..10127
                     /locus_tag="PC545_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HDF2455955.1"
                     /translation="MAKQKFYAVKKGKNIGVYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIDGSEKLSFQEDKEFIEAYVDGSYEHSVKMYGSGVVILKNNEVIKTYSEKGKEK
                     TLVSMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(10369..12498)
                     /locus_tag="PC545_001178"
     CDS             complement(10369..12498)
                     /locus_tag="PC545_001178"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDF2455956.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGPIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNDEE
                     VSKKIDNTCSWKKEHTKNIENECNCEHEHHDYLNKALDCKQEHKTDIKDDCNHEKKHT
                     KNTNKVHNSKQDKFKDKSCDEMNFNYDKDESCDKINSSYNKEDSSYEDFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKGEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGITSRPQDRENFTLLLTAIR
                     DVIGDDKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNGRKHQAN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPYLVKDGDFAMSYDDALSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            12764..13012
                     /locus_tag="PC545_001179"
     CDS             12764..13012
                     /locus_tag="PC545_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455957.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTIGIVL
                     CNARKIFDSKAIHKTRTKTVSNNKKQKSHSRDIKRKIS"
     gene            13178..14569
                     /locus_tag="PC545_001180"
     CDS             13178..14569
                     /locus_tag="PC545_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDF2455958.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIALIIVFFLSSNE
                     FSVGNEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELNKISIDVDDLLAQADRVREMYKEENTTYDIAINYIS
                     TVSILNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKAYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKDDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNTLDEKE
                     NKYKKIEILDIEKADRYVKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAISENGLNEY"
     gene            14684..16153
                     /locus_tag="PC545_001181"
     CDS             14684..16153
                     /locus_tag="PC545_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2455959.1"
                     /translation="MSNVNKKLVIAIGAVALVVIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVDFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SAFFGNLSKTISANFGKKSNLVVAINYDKNKLKAEMEKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDISKSMENFDNQTKKGNYKNELVVKATPAKVKKEQLANIDTNLGTY
                     STTFKTSQINRSINIKLATDNISNVLLMPGETFSFNKHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIVNVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPN
                     TDENKNNQNTNNQNPNNQQKPETPPTDNKPNETQPQPQA"
     gene            16218..16811
                     /locus_tag="PC545_001182"
     CDS             16218..16811
                     /locus_tag="PC545_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HDF2455960.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            16993..18339
                     /locus_tag="PC545_001183"
     CDS             16993..18339
                     /locus_tag="PC545_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDF2455961.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKSFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDTSKKEEIKKVKRKKSILPYIIVGCVLIGMGIYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPEREITVADEEDLYYQAL
                     NLKKNKEYEKAIDNFKSVVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMFYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            18521..18892
                     /locus_tag="PC545_001184"
     CDS             18521..18892
                     /locus_tag="PC545_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDF2455962.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            18885..19574
                     /locus_tag="PC545_001185"
     CDS             18885..19574
                     /locus_tag="PC545_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDF2455963.1"
                     /translation="MYNVLQTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIVGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIVGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGVESITVVAIIFTGIF
                     GGMVASTVFKLGNINHPVAKGIALGTSAHALGTTKAFELGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            20094..20960
                     /locus_tag="PC545_001186"
     CDS             20094..20960
                     /locus_tag="PC545_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2455964.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISNIIPIHDILLQSVYGGILSGLGVGVVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSNEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            21202..22011
                     /locus_tag="PC545_001187"
     CDS             21202..22011
                     /locus_tag="PC545_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455965.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVNKEIRSIKNMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYVGKFLEGKMEGKGSYTFFVDEGT
                     VMEYIGEFKEDKKNGKGNFIFSNGNEYIGYFEEDMMSGIGNMLYSSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEVLYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            22155..23219
                     /locus_tag="PC545_001188"
     CDS             22155..23219
                     /locus_tag="PC545_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2455966.1"
                     /translation="MKRLIALVLVVLIVLFYSPELLKEGYDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKSQNLQSNNTEGENLGESDIKNFEKISLGDNLSYVLSSIGKPGRI
                     DISEYGFDWYVYNQYGKEFAMVGLENDEVVALYSNSINSCENQNIKLNQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEENRVCSYLIIDKSTEDEFK
                     NLYPDDSEELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            23247..23966
                     /locus_tag="PC545_001189"
     CDS             23247..23966
                     /locus_tag="PC545_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2455967.1"
                     /translation="MTSWKKKTVYKCLIAVALFCGIVLISNFSKVSALMMDTNGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKDLI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            23990..24574
                     /locus_tag="PC545_001190"
     CDS             23990..24574
                     /locus_tag="PC545_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDF2455968.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGEAVVVKRNNEIKISDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEHFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSINHTNKGVFRGLKNPLNVTRYHSLIVDSNTVPKELEITAITDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFITEARERVHM"
     gene            24576..25925
                     /gene="pabB"
                     /locus_tag="PC545_001191"
     CDS             24576..25925
                     /gene="pabB"
                     /locus_tag="PC545_001191"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDF2455969.1"
                     /translation="MIREINTKLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVVNDTNLPFVGGAVGYLSYDLGNYIENLPRT
                     AVDDIEMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIELEEKIIDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDAHILSNSPERFIRCVNKRIETRPIKGTRPRGKDKE
                     EDLRLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDDKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            25915..26658
                     /locus_tag="PC545_001192"
     CDS             25915..26658
                     /locus_tag="PC545_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDF2455970.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIRVKNGIAVDLDIHIERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVINKALRITVFDEGYNISIRDIPYNQETYEKGF
                     KLAISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIVKLLE"
     gene            26692..27393
                     /locus_tag="PC545_001193"
     CDS             26692..27393
                     /locus_tag="PC545_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455971.1"
                     /translation="MIGCIYEVYNEEMTKIIENQNTLAVYLVGSSKDVDFTLYDVVIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDVNYFSKDGVKKLLSNREYFFVKEMKDAKVLF
                     DRENISHIIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKILNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            27419..27991
                     /gene="folE"
                     /locus_tag="PC545_001194"
     CDS             27419..27991
                     /gene="folE"
                     /locus_tag="PC545_001194"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDF2455972.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLSEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            28055..28846
                     /gene="folP"
                     /locus_tag="PC545_001195"
     CDS             28055..28846
                     /gene="folP"
                     /locus_tag="PC545_001195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDF2455973.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKVSIEMA
                     MSCGVKKEKIVLDPGVGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            28857..29222
                     /gene="folB"
                     /locus_tag="PC545_001196"
     CDS             28857..29222
                     /gene="folB"
                     /locus_tag="PC545_001196"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDF2455974.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYNVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            29215..29721
                     /gene="folK"
                     /locus_tag="PC545_001197"
     CDS             29215..29721
                     /gene="folK"
                     /locus_tag="PC545_001197"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDF2455975.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERELHRERIVHWGPRTIDVDVLFFN
                     DVVSTDERLTIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEEREEVKEL
                     VGYERKPI"
     gene            29702..30487
                     /gene="aroF"
                     /locus_tag="PC545_001198"
     CDS             29702..30487
                     /gene="aroF"
                     /locus_tag="PC545_001198"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2455976.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            30742..32532
                     /locus_tag="PC545_001199"
     CDS             30742..32532
                     /locus_tag="PC545_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDF2455977.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMNIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDVINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGILIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            32550..33716
                     /gene="rpoD"
                     /locus_tag="PC545_001200"
     CDS             32550..33716
                     /gene="rpoD"
                     /locus_tag="PC545_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDF2455978.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHDDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            33881..34570
                     /locus_tag="PC545_001201"
     CDS             33881..34570
                     /locus_tag="PC545_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2455979.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGKVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFNDFIEKKIKTYSSIVNKLEGKNGEAIDKKRKESE
                     VAIKKLENLIK"
     gene            34584..35681
                     /locus_tag="PC545_001202"
     CDS             34584..35681
                     /locus_tag="PC545_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2455980.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSGDLKGRLIQKLIKNDISLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDITKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSRKIKEKLNMKHIRVVGNLS
                     TDITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEFSEIEVIKSNVYLNPFSTI"
     gene            35744..36316
                     /locus_tag="PC545_001203"
     CDS             35744..36316
                     /locus_tag="PC545_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2455981.1"
                     /translation="MKVAVIFHSVCGSTYLLAREYKEALEEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGKEILDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGTPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSVRGGYPAYG
                     ILHIAGDNSDIRPNDDAKIGIRDYLKRLNI"
     gene            complement(36376..36843)
                     /locus_tag="PC545_001204"
     CDS             complement(36376..36843)
                     /locus_tag="PC545_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2455982.1"
                     /translation="MFKEMRLKKREMTKEDTVEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFNDSIYFHCARNGHKLDNISKNNKVSFLVVANESVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDMNLTTVVKIEIDHISGKASRL"
     gene            complement(37229..37705)
                     /locus_tag="PC545_001205"
     CDS             complement(37229..37705)
                     /locus_tag="PC545_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDF2455983.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLSVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(37825..38916)
                     /locus_tag="PC545_001206"
     CDS             complement(37825..38916)
                     /locus_tag="PC545_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HDF2455984.1"
                     /translation="MSKIECLFTIFPVLILMYATFYFMPYFVGKKHIYGVSIDQEHKN
                     YNYFIKLDKEFKKLLSLGFIIDFILVFILVFTFNKLELSYFIGIIGFLLYESILYIHT
                     HKKAKKLKSEIYSKLGHIDIDSKLIVDMDFINKKNKIIKKFKIIYLIPILFTLGMSIF
                     IAFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTDFCLMVLLYITSIGSIKSR
                     IKIDTNRIEESRVENIKYLNKIGYLFLILMIIMTTQFFTTLLSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKFNSSYSPEEDEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFSLFSLIKSLLL"
     gene            complement(38909..39298)
                     /locus_tag="PC545_001207"
     CDS             complement(38909..39298)
                     /locus_tag="PC545_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2455985.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDKYEVKYYE"
     gene            39490..39936
                     /locus_tag="PC545_001208"
     CDS             39490..39936
                     /locus_tag="PC545_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455986.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMITGAVITGVGAYAVYKMVKSHK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKEEIDLHEDAKKDNIYKEDEYKKYEEE"
     gene            40200..40925
                     /locus_tag="PC545_001209"
     CDS             40200..40925
                     /locus_tag="PC545_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2455987.1"
                     /translation="MKRKEIVMLKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSG
                     KEMISSNEYSMILLDVTLPDGTGYELCQYIRGFSQVPIIFLTACDEEVNIVMGLDIGG
                     DDYITKPFRIRELISRIKAVLRRKGNTSEENKKILKFGDLSIYTLEARVYKNDKEIFL
                     TSVEYKLLLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCE
                     PIYIETVRGIGYKWIGSENSVSI"
     gene            40912..42156
                     /locus_tag="PC545_001210"
     CDS             40912..42156
                     /locus_tag="PC545_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2455988.1"
                     /translation="MFLYNPEVKKFLSKYVTLMFIVIIISIGFSIINVSLTKEMVVRN
                     NQAIIGTLSSKYPNLESQIVDIITQGKSMENIDYGQKILIKYNYDKSIRINSEPIISK
                     LVLETIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKPFDMKNKNQ
                     EGQIGLLKTELLKMTTILNEKVELLKREKIFLNNTISDISHQLKTPMTSLIMLNDLLY
                     NDVPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHRAML
                     SMKIPMEIKNQKLTIEGNDNVSYIGDIDWSVEALVNIIKNCVEHTPEFGNIIITYKEN
                     PLFSELIIKDEGEGIDKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNGDIY
                     VNSEKGQGTEFHIIFHKMYDSD"
     gene            42236..42913
                     /locus_tag="PC545_001211"
     CDS             42236..42913
                     /locus_tag="PC545_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2455989.1"
                     /translation="MEILRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLIDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            42914..>45457
                     /locus_tag="PC545_001212"
     CDS             42914..>45457
                     /locus_tag="PC545_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2455990.1"
                     /translation="MNLYTLLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQMRETIKNDGSFHVTFYDVNRKNVEYVTKSAEIEKHAFTKQLGYSKLENSENAILS
                     IKQYDKNAINGYKVSIKEGRFPAKEGEIVLSESIINLIDDKLKIGDKITLKVGDMFDS
                     SKKKIDSMTFHEGDYIANEKDRTFKVVGIIEKPGFERYNGIATAIAYFNPMNNYNDTI
                     NVSVAVRNPKDVYKISNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYSNINNSI
                     KSIIIAVTILVIICTIATVYNSFSISINERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTKMSLQIVYNPIITIVSIIIVLLTIF
                     ISILLPAISASNISHLNVIKNTGEYKVGKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IITLFSLMVSIIIFISFSGFTLLLLRGEEIRNSQRKYDLYLTAKNTARSVEDTISELE
                     DIDGIKNFELATGQYVSIRVSENKINKSKEDLIRKYYQKYKIGDSYEYDFSNNELIFP
                     GDFAVKNKINNLVQGSFNKERAIEENGVILVRKSAFEENGKKGVVELTNYKVGDTVNC
                     EYLDENALSKKVKVKILAITDEERLGLGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESASNESEKQDLKVIKIFVYGFIVVISLVSVTNI
                     LNTVSTSINLRKRELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGIILIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPMSKLNKENIIDNI"
BASE COUNT        18447 a         4634 c         7654 g        14722 t
ORIGIN      
        1 gagtaataat gttgatttaa aataaaaata agatttcgga ggagtttatt atataaatga
       61 aagattacta taaaatagga gagatatcta aaatatatgg aataggtaga gattcattaa
      121 tgtattatga agaaataggc attctaagac cagttagaga tataaatgga tatagaatgt
      181 ataatatatc agatatatgg aaattaaatt taataaaaga atttagaagc ttgaactttc
      241 caatgaagaa aataaaagaa tacctagatg acagaagtat agagtctacg aaaaatattt
      301 taaatgaaga actcgattta attgataaaa aaatagctga atttataagc cataaggaaa
      361 atattataaa aagattgagt tcaattgaaa gtgtaattca aaatacaaaa atagatgaga
      421 tagaggttgt atatatagaa aaaagaaaag ctttagaatt aaatgctgat ataaagaggg
      481 atgaagactt cgatttttta attcaaaaac ttcagaaaga atatgaagat agatttaata
      541 tacttggaaa caacaatatt ggttctgcct tttctactga agcaattaat aaaggtatat
      601 ttaatgagtt taaatctgta ttttgttttc tggaaagtaa tgagaaagta tataatatag
      661 tttttgatga aggatattat gtaactttaa attatactgg gagtaattct aataataaga
      721 tatatatgga gaaggtgttt aaatttatag aagaaaataa ttataagata attgctgacc
      781 ctatagaaat ctataagata gatatacatg agactggaat tgtagaggag tttgtaacgg
      841 aaattcaagt tcctataact aaatagctat atttcaatgg attagatggt aaatagaatg
      901 aaaaaatgtt gatttgccac tgtgttataa aatcggtagc aaattatatt ttaaattata
      961 agatagcatg aaaaaataat tgtttagggg gttataaatg tgaaatgttc taattgtgga
     1021 agtgtaaata ttgaaaaggg aattttaaca actggtggca tatatgctga aactattgga
     1081 ttaaaatata gaggagatgg aaaattagat aaagttataa ataaaacttt tccagtacaa
     1141 tctactatat attcagattt atgtttagat tgtggagaaa tagttagaac ctatataaag
     1201 gataatactg ataggaattg gtgtaaagat aagaaataga ataatttttg aagtatatta
     1261 atatttgaag ttgaatttta ttaaagatac aagtgaaaaa gtataaatgg gaagaaattg
     1321 ttatatggaa gaaaactttg catatagggg agattagttt tttaatataa taacattttt
     1381 aaatgtagat aaaactaaaa agggttctct agaaaacaat ttctatttaa ttatacttac
     1441 ttaccaatac catcacttga tgtaaacaag attctactac attttctccg tactctagta
     1501 taaaatcatc tagctgtgta tctataaaat cacaataatc atataaacta ctgatataag
     1561 cagttaacat gtgtgtacat ttatccattt catctctaaa catatatatt actatccccc
     1621 taaaaaactt tcataaaaca aaaagaacaa tatctttttg gcaactggct gacataactc
     1681 atagtttatt ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt
     1741 gccgatttag ttttattcta caactaaaat aatacactag gataagttat tgttcaacat
     1801 acttgtcaga aaattgaaat atttaacagt atcttaacaa tatgaaatct atttttcact
     1861 caagaaataa actatacatg tagaatattt aataaaaatt atatttagtt ttgtattgaa
     1921 gtttttgtat taaaaagttt agttcacaag agttacttgg ctagataata gatgaacaaa
     1981 aatatcttga aaaaatatat atgaatggca aaaaacaagg gatgaatttt atatagactc
     2041 atcccgtaat tatttatata ttgaccttga ttaaatatat tattattttt aagcttcaat
     2101 ttttatatta tatttctaaa gtttctataa gaggattatc gctaaataat tttaataaat
     2161 ttgttttttt atatccttta ggtaacttag tatatataac tacttctaac caatccccat
     2221 caaggcgttt aatattcatg tttataattt caatattaga ttttttaaat ttaccttcaa
     2281 tatattctat ggaatcagtg ctgttagaaa ttttaagaga aacttgctct gctgtaggga
     2341 gtttcatcca tttcctatct ctatgaagtg ttatctgtac aataacaata atgatacttg
     2401 aaacaatacc aactaagtat aatccagagc caatggcaag acctgtacca gctgttgccc
     2461 atataccagc agctgtagta agaccactta ctgattgatt tctaataaat attaatccag
     2521 cacctaaaaa acctatacca ctgacaactt gtgctgcaac gcgggctggg tctagaccaa
     2581 tacctggagt acccactata tcattaaatc catacttgga aacaatgatt ataagtgctg
     2641 aagctaatgc cactataaaa tgagtgcgta ttccagcttc ttttagtcta ttctttctct
     2701 catatccaat taaagcccca cagaaaccag cagtaatgat tcttgctata tattcaaatt
     2761 gaatcaatat atgatggttc atttatctag cacccccttc tatataatat tatatatact
     2821 ataatactac attttttatg tgaatatagt aaagtttaaa atttatataa tagttatata
     2881 tgaatataaa atgtataaac gtatattatg tatataaaaa tgatagaaag catctaattt
     2941 taaatgatta aagagcaagc ttaaataaat caaacttgct cttttaacat agtgtatgtt
     3001 tttatttgtg atagttagat aaaaatttat ctgtgttttt tacatctgca aaactgttct
     3061 gtagtgatgc catgaatact tgatgtacag tatttgcatt aatttctata ttattccaag
     3121 ataaattctt cgttgtacat gcatcgctta ttagagttat gtcgtatcga agtttctttg
     3181 ctgttcttac acttgtatca atacacatat ggctcatcat tccacagatt actagattag
     3241 taatattttt tttctcaagg atactttgca aatctgtttc aaagaaacta tctggtgtgt
     3301 gtttagttat aatttcttca ttatctagtg gaaaaacaat tttatttatt tctgaaccat
     3361 gagtattagg aagaaaaaaa gaagctgttt tatctgttga tatatgctgt atatgaataa
     3421 ctggtaaatt gttttttcta aaaaacagta gtattttttt agcattttca gcagtttctt
     3481 ctgattgaaa taattcacat tttccatttt taaaataatc attttgaata tcaatcaata
     3541 ttaaagctat agacatgatt atacctccta tttattttta ttataatact attgtaaaaa
     3601 atgtaaatta cttacttttt ggtatgtata ggattactta ttgtagcaaa ttcctttatt
     3661 atctagaatt tcttgttgac catgttcaag catatattcg attcctattt cttgcattat
     3721 ttccactgct tttaacattt ttttaccacg attagtaaga aaatattcaa catgtaatgg
     3781 atatccagca gaacttattt tatcaataat cccaaaatca cgtaactctt ttaattgttg
     3841 aatcaacatt ttttgattta ttccataaat attatgttgc aattcagaaa atgttttatt
     3901 agattgtctt aattcaaata taaggatagt tttccatttt cctttgatta tgtcatgtac
     3961 aatttcaaga agacatgtat attcagctct tattttcata gcatatcacc cccagtaaaa
     4021 tgataataaa ttatatcact tgtcttgtga tatgacaaat atatatgaac tgtatataaa
     4081 ttttatgttt tattaagttc taaatttctt ctattgtagt tcatatttag tacataaata
     4141 attagtaaga tataagtaaa taaatatata acatgactat agaaatttaa ctttaaaata
     4201 acttatgata atttagagga ggaatatata tgaatatatc aagaaaagct atgaaaataa
     4261 tagaattagc acaaaaaatt gcaaataaga gaggtatttc agtagaagaa gcttggagtg
     4321 aagcagtaac agaatataaa aataaatacg aacatattgc atagatattt aaaaatttag
     4381 aatatttggt agaatataat gattttaaat gatactataa attataaggt atatcattat
     4441 taacatagaa ggaggatatc tgcttgattg taaaaataga taatacttta gaaaaagaat
     4501 tttggcaata tgtatcacat gaagaaagtt taaatttatt cataattgga tacgttgaga
     4561 attatggatt tagctctcaa tatcaagata tatggtctca ggtagaagat ggaaatataa
     4621 cctctataat attaaaaaat aagtcaacac ttataattta tagttttaaa aataatttta
     4681 atataggaga aatgaagaat catataaaag atttagatgt agaaagcata agtggaaaaa
     4741 aatgtgtaat agatagacta ataagtaaat acaaagattt ttatgaaaaa ctagataata
     4801 aattttgtgt attaaaagaa ataaaagaaa tagatttttc caacatgaag gagtataaaa
     4861 tagaaaatgc tcaggaaaaa gatattgatg aaataggaaa acttttaaat aggtcagact
     4921 ataaagtaag taaaaattac atagaagaaa gaaaagttca tttaaaagag ggaaatgtaa
     4981 gagcatattt tattagaaat gatgatacta tgattagcac tgtgtcaact ggtatggaaa
     5041 ctagtttttt ggcaatggtg gtctctgtaa gtacagataa aagatatagg ggaaaaggac
     5101 ttgcaagtta tatggtttac aatttgagta aagaattgtt gttagaagga aaagttccat
     5161 gtctttttta taataatgat gtagcaggta aaatatatca taatatagga tacaaagaga
     5221 taaatgaatg gacaattctt tttaaatagc tataaaaaat aaaaaactct atcgagaatt
     5281 aaagtattta taaataacta tatttttact ataattcatg atagagtttt tttaaattta
     5341 tgatactatt ttttattatt taaataatca agtgttttaa gtttataacc ttctttttcc
     5401 caatgtgtaa gaacctcatc taatatctca gtatttgttg atgaatttgg atgcagtaaa
     5461 actattgcac caggatgagt tcttgaataa attttgtttt tggcaaattc atgagtaggt
     5521 tgtttctttt cataccaatc tacataagca aaactccaaa atattgattt atatcctaaa
     5581 tcttgtgtat attttaaaga ttgttcgcta aatttaccca ttggaggtct aaaatactta
     5641 ggcatttctt ttccagtaac atctttgtat gctttttcta cacttgtaat ttcttctttg
     5701 aatttttcaa aatctgtaat acctgccatt gatggatggc tttttgaatg attacaaact
     5761 aaatgtcctt ctttttccat acgctttatt aactctggat tgctttttat ataactttca
     5821 actacgaaaa attgagcttt agcattgtgt tttttaagcg tatctaataa ttttggagtg
     5881 tttccacttt catatccagc atcaaatgat agatatataa ctttttcatt gggatttcct
     5941 acataataag ctccgtattt tttaaaaaaa tcagcttctt ttataggtga tggctgttta
     6001 ccatcctccc tagggttaaa ataccaatca tattcatgtg tgtcgagtgt tggactagaa
     6061 atttgagttt ttgttttatc taatgattta aaatttatac tagcaattcc aaaaagtatt
     6121 agagcaaatg ctcctatcat aatgtatttt tttaggctat cttttttcac aatttcacct
     6181 cttatttaaa atttaaagta tatttcttat aatatagttt gaatatttat agttaaaaat
     6241 attcacatat acaaataata aatgaaagaa gtttactgta agatataaag ttgatattaa
     6301 ttatcttaaa taaagtgtat ataaatgata aaatataaag agaaatgatt gtaaataagt
     6361 taggctggtt taacttgaag ggatataaaa atggataata taataagtgt attgaatgat
     6421 gttgtttttt tagatataga agttagtgga ttagattgtt taaattcaga aattttagaa
     6481 gttggagctg tcaaggttaa agactggaaa atatatacct atgaatcatt aataaaaaac
     6541 aaatttgagg tacctgtaga agtattctca gtatgtaaaa atttagataa aaatgattta
     6601 gaaatagcta atgaaataga actggtggaa gatagacttg taaattttgt agaagatagt
     6661 ttcattatat gtcatgattt aagtctgaaa aaaaagtttc ttgagtatca tatgccaaag
     6721 ttaaaaaaca aatttattga tttaattgaa ttagcagtta ttttagaacc atatcataaa
     6781 gattatagct tagaatactt aaaaaatact ttaacaaatt gtaattctaa agtggaaaat
     6841 agagctttat cagatgctat tgatataata aatattgtaa attgcctttt agtaaaattt
     6901 aataactatg aaaaaactac tttggagcca ttgagtttta aaataaattc atatcttaaa
     6961 aaatttaatc ttccaacatg ggaatggagc aaatttttag aagaggcaaa ttatgattta
     7021 tcaaataata taaatataaa aaaagaatat aatatatttg actcaaaaga agaaaagaag
     7081 aaggaaagag aaaccttaaa aattttaaat gaagaagaaa aaaattatga agaattatta
     7141 aaatacaaaa ctatttggga gaataaagaa ggatttacat atgaatatag acctggtcaa
     7201 tatgagctta ctaaaacaat aagagagttg tttagaaata gcgaagatga agaaaaaatt
     7261 gcatgtatag aggcaccaac tggtataggt aaaagtgtag gatatttatt gcctgcaata
     7321 ttagaggcta gaattaataa aaaaagatta ttaatttcta ctgacacaaa ggaattgcag
     7381 atacaattaa taaataaaga tattccaaat gtgttagatt ctttgggtct aaatggaaaa
     7441 gtaagttacg gatatataaa gggaaaaaat aactatattt gtatagatag actggaagca
     7501 tatatagatg attatgagtc tcaaaaccct actaaaggag aaattttatc tttaatattt
     7561 ttgaagagat tagttgaagg tggcaagtat ggagatatag aagaaataaa ttatttagtg
     7621 tttgacaact tcaaggaaat aagtactcac ttaagaaatg taagttgtga cccaaatatg
     7681 tgcagaccta aaaaatgcaa aaaagattgt ttatataaaa acagaataga agaattaaaa
     7741 gaagaacata taacagtagt aaatcattca ctattagcta aatggccata taaggatgaa
     7801 aaacctttag aaaatataat agtagatgaa gcacataatt tgactgaaaa aggatatgat
     7861 ttcttttcaa gcattataaa ttcaaagtct ttaaggtatt tattacaaga aatttatcct
     7921 tacgagttta ttcaaaatag tagctttata tataaaaaat atagtagaaa tatgagaaaa
     7981 ataaaggctt ttgataaatt ttataatgta ttaaaaattg gaagagaaga taaacaaaaa
     8041 attgctagaa gtataaatct cattattgaa gagatagatt caatattaaa gtttggtaat
     8101 tgtaatgaat ataataatgt aagtaattat aatttaagat gggaactgaa tttgcaaata
     8161 gacgaaattg tgggaaaact aaaaaaggat ggtatagata ctgaaataag ttatagagcc
     8221 tatagtgaaa agataaagtt gagttgtgag aaaataataa aaaatttagt ttcaataata
     8281 ataataattt ataggaatat tgatgatgat agtatagata aagaagcaga tatatataaa
     8341 tttggaaagg caaagacaag agatttagag gatataaaga ttatatttga aatcttttta
     8401 gagtatgatg aaaaggatga ttatgcaagg atagttgaaa ttgataagaa ttataatgac
     8461 tttgaatttc gagtagtgcc attaaaaata gcagatttat ttgaagaaaa tattttatct
     8521 caattagaaa aaggaatatt tttatcagca actttgagtt tatctgaaag tatgtcttat
     8581 tttaaaaata cattaggaat agacagagtt aagaatgtgg aaaagattat agaaccaatt
     8641 tttgattata aaaatagagt ctctgtagtt ggattttctg atatttgtga gtacagaaat
     8701 tctgaatttc caaatgagat gagtaaaatt atatctaata tatcaaagat tacagaaggt
     8761 catacattag ctctttttaa tagtaaagat agacaagaaa aaacttatga aatattgaaa
     8821 aaatacttac atagttttaa cttagaaatt tatgctgata aaaaggggat tagacacctt
     8881 aacgatttaa atagaaaatg tgtagtactt ggctcaaaag gatgttttga aggcgtagac
     8941 attccagggg atggtttggt ttgtgtaact ttagacaagc ttccgaatct aaatccaaaa
     9001 gaccctttgt attttactat aatgaagaag tatggtatag actactatac tataaattat
     9061 cctcaaatga ccataaaggt aaagcaagct atggggagaa ttttaagaag caaatatgac
     9121 tatggatgtt ttgtgatttt tgatgtagga actaatataa gtgtgttaaa gagattagag
     9181 aaagatttac acgattgtaa aatttcaaag gttaatagca atgagtttta tacttatata
     9241 aggcgacatt taaataaata tagaagttta atactaaaat ctgttatatt tgatactata
     9301 aaagcgttga atgttgatgc taaaatggat aacaatgatg ttgataagga tattataaaa
     9361 aaagatataa atgaaaatat aagacaaaga gctgtaaaag gagaagtgta ccatattgat
     9421 attataaaaa aagatatgaa agtaaaatat tttgatagaa actatcttat aaatttagat
     9481 atatttatga gagaagaaga taaaaattaa gagtgggagt gatatatttg gcaaaacaaa
     9541 agttttatgc tgtgaaaaaa ggtaaaaata taggtgtata taatacatgg gatgagtgca
     9601 aaaaacaagt aaatggattc tcaggagctg aatataagag tttttctact tttcaagaag
     9661 ctaaagaata tattgatggg tcagaaaaat tgagttttca agaagataag gagtttatag
     9721 aagcatatgt agatggaagt tatgaacatt cagtaaaaat gtatggttcg ggtgttgtga
     9781 ttttaaaaaa taatgaagtt ataaaaactt atagtgaaaa aggaaaagag aaaactttag
     9841 taagtatgag aaatgtagct ggagagatag aggcttctaa aattgcaatg cagtactgta
     9901 ttgacaataa tgttcaaaac ttgattttat actttgacta tgaagggatt gaaaagtggt
     9961 gtactggagt ttggaaaaca aataaagaag gaactatagc atataaaaat ttctatgaca
    10021 gtattaagaa taagcttaat gtaaaattta caaaggttaa agctcattct ggaaataagt
    10081 ataatgagga agcagataaa ttagctaaaa aagctattgg tgtataataa aaaaatattt
    10141 atacataatt acatggtata agagtatatc taaattccta ctttataatt aaacaacttg
    10201 taattaaaca acttataatt aaataaattt tgattaaaaa agcagattgt attatgaaaa
    10261 aataagtatc taatttataa acaattaata atagtattaa ttagatacaa cttatttaat
    10321 ataataaaat ctgcttttta tataactata aaattttcct acattctatt agaattgccc
    10381 ataaatacct tcaagttctt cttttaatac ttcagggtca tttataccaa tagacatggt
    10441 tctagccagt atatttgctt ggtcataagt tgatgtccat gagaatacac cacctagaca
    10501 atttctaaga acatattgag tttttaagaa tattgataaa gcatcatcat atgacattgc
    10561 aaaatctcca tccttaacta aatatggaac ttgagcagta ttatcaaatc tatattcata
    10621 tccattttta tttatataat cccttctaag ctcatcataa gttcttgtta tagtagcacc
    10681 taatcttcca taaaatggta taccaagaag gattttttca gaaggcattc cagcattctc
    10741 aagatttctt accattgcat caacactata tcctggcaaa gataagtctg aatcaaaaag
    10801 atttgcttga tgttttctac catttgggcc tgtttcacct gctgtaaaat cataactcat
    10861 aagattaaaa taatctatta taggagctat tttatctatt tcagcacttg aatttatata
    10921 tcctctatct cctgttccag ctacactaag ccatttatca tctcctataa catctcttat
    10981 ggcagttagt aagagtgtaa agttttctct atcttgaggt cttgatgtta ttccagatgc
    11041 actacttcca ggatattccc agtctatatc tattccatct aaagcatatt catttatcat
    11101 ctgattgacc tgtcttgcaa aattatatct agatgtaggt gttaatgctg catcagagaa
    11161 tccttcagct ccccaacctc caatagctac aattactttt aagctaggtt tttcaccttt
    11221 aagagcaagt aattgattta aaaatctagg ataaggaata aacaaatttc cagactggtc
    11281 tatctcagca aaagcaaata taactgcatc taatagttga gcatctacat tagttggatt
    11341 tccaagaaca tattctgcta caattggatt tatatctgga cagtttgcat tttcaactat
    11401 tggattgtat gggtctgtta tttgtggtct aactaatttt ttagaatctt ttaaagtttt
    11461 catagctatt tttttagtat ttttttcgct agtataatcg tagtttttat aattatgttt
    11521 atagaaatct tcataactac tatcttcttt gttatagcta gaatttattt tgtcacaaga
    11581 ttcatcttta tcatagttaa aattcatttc atcacaagac ttatctttaa acttatcttg
    11641 tttggagttg tgaactttgt tagtattttt agtatgtttt ttctcatgat tgcaatcatc
    11701 tttaatatca gtcttgtgtt cttgtttaca gtcaagagct ttgttcaggt agtcatgatg
    11761 ttcatgttca cagttacatt cattttcaat gtttttagta tgttcttttt tccagctaca
    11821 agtattatca attttcttgc tcacttcttc atcattataa ttatctggta caaaacataa
    11881 gtaccagtcc atacatgaat atttcttatt acaactgtta actctatttt ttaattcctt
    11941 atagttttta ggataaggta aaatcactgg cattccagga aaccagtttg caggagtaac
    12001 tatattatct ctatcactag tctgaagtgc atctactatt ctaagtattt ctggaatatt
    12061 tcttcctgta gttagtggat aataaagaat cgttcttaga atttgattat tatctataat
    12121 aaatacagag cgaacagttg atgtatcact cattggtttt gatatcatgc cgtataactt
    12181 ggcaattctc atatctctat cttctataat aggaaatgga atttctacac ctgtaagtaa
    12241 agaaatatta tacatccaag ctaaatgtga actgttacta tcaacactta gaccaattag
    12301 ttctgtattt ctttttttaa attcgtcata atatttagca aaacataaaa attctgtagt
    12361 acaaactggt gtaaaatcac caggatgtga aaataaaaca atccaattac ccttatagtc
    12421 agagagtcta ataggaccat ttgttgtatt ggctttaaaa tcaggagcct ttgaccctaa
    12481 acttggcaaa tttggcatgt aaatcacccc tcaataaatt atcatttatt ttattctatt
    12541 aattaataca tattagtgtt actattttat taaaagttaa gtaatcctta tgtcaaaaaa
    12601 agatatttat taaaaagatt taatattttc ctaatataaa tgacaatagt aattgctatt
    12661 tttttactaa tttggtatag taatggataa ggggtagtgt caattttagt gattatacga
    12721 ggtgagtgga ttgaaaaaaa ttctaagaat tttagttatc tccatgatga tatgttttgc
    12781 aatgactttt gcattagtgt taataaattc agttatggga gtacatatag gattggatta
    12841 ttactttttg atgggaagtg tgcttacagt ttgtttaaca attggaattg ttttatgcaa
    12901 tgctagaaaa atatttgata gcaaagctat acacaagaca agaacgaaaa ctgtcagtaa
    12961 taataaaaaa caaaagagtc attctaggga tataaaaaga aagatatcat aatttatgta
    13021 aaggaaatat ttatgtataa aataatgatt gataggtaaa ataaaaacac gatttaactt
    13081 gtgtttttat tttttttcaa gaattatgat ataataacca ataagaaatt tagtttacca
    13141 taaaatataa agattattaa atggagaggg aactataatg aattttagag atttatttga
    13201 gaaggctgta gattttattg atgaaaaaag gaaatctata gttgcgattt cattatcaac
    13261 attaggtgta attgctttga taatagtatt tttcttaagt agtaacgagt ttagtgtagg
    13321 aaatgaagct aatgagttgt taaagattat agagaaaaga cagtattcta tagctgttga
    13381 ttactacaca agtatagaaa aaaagttttc tgattcaaaa atggaaaggt ttaataagtc
    13441 tgtttctaaa aaaataaata aattgctatt aaatagtggt gataaatatt tagatggaga
    13501 cataagtcaa gaaagtttta ttggattaat aaataccgtg aaagaattaa acaaaataag
    13561 tatagatgtg gatgacttat tagctcaagc tgatagagtt agagaaatgt ataaagaaga
    13621 aaatactact tatgatattg ccattaacta cataagtact gtttcaatat taaatggtat
    13681 gggtagtaac ctagatgtat acaaacagaa tatagaaaca atcaaagagt ctagagatgt
    13741 ttatgactca gcagtaaaag accaaaaagc atataagtat tatgaagcta ttgaaaagta
    13801 taataaagta ttaaaagaag atgaaaagta ttacagtatg gctcaaaatg ggaaagaaca
    13861 atgtattgaa gaaatgtatg attactatat aagtagagct gaagaggcaa atacttctgg
    13921 agattatgaa agagcattac aatatattga gtatttaaaa gacgattatt cagatgatga
    13981 aaaggtacag agtttagaaa aaaaatataa aaagaattta tcattgtata ctttaacatc
    14041 tgatgacatt ataaatgtaa tagctaaaaa aagtggcaaa gacaaggcaa atctatctat
    14101 aaattcactt ccacaaatga taaaaaataa taaatactat tatgctgaag tatatgagta
    14161 tgataaatta ataaatgaag ttttggtaag tgcaaaaact aaagatttat attcatataa
    14221 agatggtaaa aaagattaca aagtagatta tggtgatgga tactttagaa tattagaaga
    14281 tggaagttat caatttggta ttacaaaaga taaagctaag tttgtgttaa ctaatactct
    14341 tgatgaaaaa gaaaataagt ataaaaagat tgagatatta gatatagaga aagcagatag
    14401 atatgtaaaa agtaaaaaga gtttagaaga attgtttgga aaatataaaa atatttatta
    14461 ttatgctgta gtaaataaag gcttatttag aggcaaagaa gtttatgcta ttaatattta
    14521 caatgaaaaa atatatgcca tatcagaaaa tggactaaat gaatattaat taataatatt
    14581 aaattaatat ataattaaaa agtttttggg gttttaaggc gcattatgta gtaatataat
    14641 aatagggata tataaataat tttattgtaa ggagaagtat gggatgagta atgtgaacaa
    14701 aaagctagtt atagctattg gagctgtagc attagtagta ataattttta taggcataat
    14761 gtgcatgcaa tttaaagggg agaaaatagc aaaaaacaca tatgttaatg gagtaaatat
    14821 tggaaaatta actaaatcac aagcaaaaca ggaattagct aaaaaataca aattagagaa
    14881 tgttgatttt aattacaatg ataaaagctg gaaagtaaag agcaaagatt taaatttatc
    14941 tactgacctg gataaaacag tagaaaacgc atataattta aatagaaaga gtgctttctt
    15001 tggcaatcta tcaaaaacta tttcagcaaa ttttggcaaa aaaagcaact tagtagttgc
    15061 gataaattat gataaaaata aattaaaagc agaaatggaa aagatagcta aagaaataga
    15121 tgtagatgta aaagatgcta cacttgatat aagtggtgaa aaagtcaagg ttataccaga
    15181 ttctgatggt cttaaaatgg atatttctaa gagtatggag aattttgata atcaaactaa
    15241 aaaaggtaac tataaaaatg agttagtagt aaaagctact ccagcaaaag taaaaaaaga
    15301 gcaacttgct aatatagata caaatttagg tacgtattca acaacattta aaacttctca
    15361 aataaataga agtataaaca taaaattagc aacagataat ataagtaatg tattacttat
    15421 gccaggagaa actttctcat ttaataaaca tacagggaaa agaagtaagg aaaatggata
    15481 taaaagtgca cctgtaataa tggaaggtga gatggaagaa gattatggtg gaggagtttg
    15541 tcaagtttca tctacgttat acaattctgt gctatacgca ggtttagaaa ttgttaatgt
    15601 aaaaaatcat actataccat caagttatgt tccaaaagga agagatgcaa ctgttgcaga
    15661 tagtggaata gatttcttat ttaaaaataa tctaaagcat ccagtttaca taaaaaatta
    15721 cgtttcagga aatcaaattg tttgcaatat atatggaagt gcagaagata aacaaaatat
    15781 aacaatatca acaaaattag atggcgtttc acaaactaca atgaaaagag ttaatgaccc
    15841 tacaatgcct aaaggtaaag aaaaagtaga caaaagtgga agaaatgcat attctgtatc
    15901 aacatataga acatttaatg atgcaaatgg taaaaaaatt aaaactgaaa aaatagctaa
    15961 ttcatattat cctaagaagg aaggtattat attagttggg actatggaac caaaaccaga
    16021 ggaaaaacca aatacagacg aaaataagaa taatcaaaat acaaacaatc aaaacccaaa
    16081 taatcaacaa aaaccagaaa caccaccaac tgataataag cctaatgaga cacaaccaca
    16141 accgcaagct taaagttctc aagaatagga tttatgtaga gtaataaaaa atataaatct
    16201 aaataggagg gattactttg aatttaaata atataacgaa ttacgttcaa agtatttgcg
    16261 agaatatttc taatgtcttg gatgtagatg ttactttagt tacaaaagat ttaactagga
    16321 tagcaggtac aggaatattc agaggtaaaa taggagagac aatatcagag aagtcagtgt
    16381 actctcttgt tttaaaagat gggaaatcct atgttataaa taaagaaata gaagagaact
    16441 gtaataaatg tgcaaataga gaaaattgta aagagttagc tgatatatgt actcccgtta
    16501 aaatgggaga gcatatactt ggaatcttgg gtatagctgc gtttaatgaa gaacaaaaag
    16561 aaaagatact ttcaaaagat aaggagttaa ctgcttttgt aaggagtatg tctgatttaa
    16621 tatcttttaa actagatgaa ctttataaac aagaagttaa gactatagat gaccttgaga
    16681 gggaagctat agaaaatgcc ataaagaaat atggaagtaa tacagaaggt atgaaaaaag
    16741 tagctgaggc attaaatata ggtatagcta cactctatag aaaagtaaaa aaattcaaca
    16801 ttaaaagtta atagtattga aaaagaggta acctttttat gaaaagaaga ggattacctt
    16861 ttttattatg agaatggtgg aaaaatatgt atagttttga cttgacatat taaatatgtt
    16921 atcttaaata tatattttag agtggaggtg agtattattt tagttaaaga tataaaacaa
    16981 gacatagaaa gcatgcctag aaaaatttta gatatatctt tgatagaaga taataaaagt
    17041 gatttgactt tttttaatat agaagaagaa gacggtgatg aaacattaaa agcattaaaa
    17101 cagagttatg tgactacaaa ttataaagaa gaaactctat taaacttgca aggagcttta
    17161 aattcaaagt cagatacaac gtctaaggag ttaaaattag aatcatataa aagatataac
    17221 aatgcattgg atttagcata taaaaattat ataactagtg ccattaatat ggttaataaa
    17281 gctctggaga taaatcctaa agacattgat atactaaatc ttagaggtct tttaaaatta
    17341 ttaaaatgtg attttgctaa atcatttgag agtttttata ctgctttatg ttatgaaaat
    17401 aatcatatat ctagaaaata tgtaaatcta atttcatctg aagattttaa aactttttta
    17461 ggaagataca atcattctat aagatttata aatgaagaat taaattatga gtcaatacat
    17521 attttagaga acataattga agaagaacct gaactaatag agccatatgt aattttatct
    17581 ttgttatatg ataaactagg taatgtaaaa aagagagaag gatatttaga caaacttaaa
    17641 gagctagata aagataatcc tgtatttgaa aaaaatgaag aagaaaaaga agatacaagt
    17701 aaaaaggaag aaataaaaaa agtaaaaaga aagaaaagta ttttgccata tattatagtt
    17761 ggctgtgttt taataggtat ggggatatat ttgattcaaa gcaaaaagag aatagaaaac
    17821 ttgaataacc aaataagtag taaagaggaa aagttaagtg aaactgataa aaagttagga
    17881 gaaacgagta aaaaattaga aaaaacgaat aaggaattaa atgaggttaa aaataaagaa
    17941 ccagaaagag agataactgt agctgatgag gaagatttat attatcaagc tctaaattta
    18001 aagaaaaaca aagaatatga aaaagccata gataatttta aaagtgtagt ttcttctggt
    18061 aaaactaaaa aatatatttc tgagtcaata tatcaattag caataacaaa taaattatta
    18121 ggaaataagg atgaagctat aaaatattac aaaaaatata taaatactta tactaaaaat
    18181 gaccagtatt atgatgattc atattacgag ttgggtatgt tttattatga taatggcgat
    18241 ttaaaaaatg cacaacagac tttttatagt ttgagaagtg aagttccaga cagtatgtat
    18301 aacaattcaa aaataaaaga aattttaagt gaaaaatagg tttatatagt taagtttttt
    18361 gaaacaacca agtatgtatt ttattaaatt aaaataaaaa taaatttaaa aagagattgt
    18421 gtcaaattat aactttctat tttaaagaaa gatatttgct tgttgataaa actacaaata
    18481 tatgatttga agcaatctct ttaatttttg gaggtggaaa atgaaagttt tcaatcaatt
    18541 ggtaataatt ttatctatct gggtaatagg agagtatata agttccttta tacaaggaat
    18601 tatacttatt cctgggagta tagttggtat gctattatta tttctactac tacaatttaa
    18661 agttttagac ttgagtagta tagaaaatgt aagtggattt ttcctcgata atatggctat
    18721 attttttatt ccagcaggtg tatcacttat aaagtcatta gacctaataa gggaaaatgt
    18781 attagtgctt ttattagtca tatgtttaag tacactaata gtaatgtata ctactggaat
    18841 tattgtggag aaaatgataa aaaagaagga agaaggacaa aaaaatgtat aatgtattac
    18901 agacaccagt atttggtata atagtaacaa tagttttttt caatttagga agatacattc
    18961 aacgaaaaac aagtaatcct atatgtaacc ctttattaat tgcaatcgta ggaataatat
    19021 tgtttttaag catatctaaa atcccttatg agaattacaa gataggagct gatagtctaa
    19081 atttcttttt aggtcctgtc acagtagtac ttgcagttcc actttataaa caatttgaat
    19141 tatttaaaaa gcatatgttt gagattattg taggaatagg ttgtggtata gtgatttctt
    19201 ttgtatctgt attgataatt ggcaaaatag ctaattcaga tgtaagtata ataaattcac
    19261 taattccaaa atctataaca acaccaatgg gaatatcttt agcaaattct ttaaatggtg
    19321 tagaatcaat aacagttgta gccataattt ttacaggtat ttttggtggt atggttgctt
    19381 ctacagtatt caaactgggg aatataaatc atccagttgc taaaggaatt gctcttggaa
    19441 catctgcaca tgctttagga actacaaagg cttttgaact tggagaaata gaaggagcta
    19501 tgagtggtgt ttctattgga gtctctggta caattacagt aatactaatt ccaattataa
    19561 tgaattttat ataaaaaata aaattaagat gagtaccttg aatacttaaa acaaaataaa
    19621 ataaaaaaaa tttaaaataa aaactgaacg aaaaaaatgc aaataatgaa ataaaaaaat
    19681 catgtactga aattataaaa agaaaaattt catttaggaa ttaattataa agagacaatt
    19741 actaaacttg aatttgctaa aataaaataa attactgcta aaatagatat ttagaaaaaa
    19801 atactataaa tttactatat tgcaagttaa ataataagca gtatcatatt atgctagaaa
    19861 aagtttcatt taaggcataa atgagatgat ttgaacgttt ttaaagatta gaatataaaa
    19921 gattgcacta gacatatatg aaaaagataa tataatagat gatgtaatag atgacagttc
    19981 aagaaattat aaaaaaaatt tatttgaaat attatctatt acatcaggaa taatatatgt
    20041 aagagtaagc ttttaaaatt ctaaagattt agaaagattg aagggagaaa gaaatgagtg
    20101 aagttaagaa taccctacaa aaacatccag ttttaagata ttcaacaatg ctatttggga
    20161 tgttgatgac atctatagga ataaatgggt tcttaagacc agcacatctg cttagtggag
    20221 gtgctacagg tatagcaaca tctataaact atttgacaaa tataaatgta ggtttattaa
    20281 catttttaat caatattcca atatttatct tagggttcat ataccttgaa aaagagtttt
    20341 gtatttcaag tttggtaaat atgattgtat tttcattatt attaggagca actcaggaaa
    20401 taagtaatat tatacctata catgatatat tgcttcaaag tgtatatggt ggtatattat
    20461 ctggtttagg agtcggagtt gtatttagaa ctaggtcttc tcaaggtggt acagatataa
    20521 tagctgcaat actaaaaatt aagaagaata tagaaatgaa agacactgct ttagcaataa
    20581 atggcttgat agtacttaca ggaagttttt tatttgggtt ggatttagca ttatacactc
    20641 ttataggtct atttctaaat gcgtattcaa tgagttttat taaagatgca atgaattacc
    20701 aaaaatctgt tatggtaatg tcaaatgaag tcgatttaat agcagaagat ataatgaaga
    20761 gtttagttag aggtgtaact tttttagatg cagaaggtgc atacactcat caaaaaaaga
    20821 agattatata tacaatagtt tcatcaaatg agataccaaa gataaaagat atagctttga
    20881 agtatgataa aaaggccttt atatctgtaa atgatgttac agaagtaaag ggaagagggt
    20941 ttaaagcaaa agatttatag aaaattttac aagctgggag tttataatgt aaatttgtat
    21001 attatatctc tcagcttatt taaatttagt atacgaaaat aaaggtttta ttaatgattt
    21061 gtgtatcact ttaaaagata tcattttaaa attaaaattg aaaaaattac ttaatatgta
    21121 gaaaacatga ccactattag gatataataa atttaataga tttagtaaaa tacctaggaa
    21181 taacgttgga ggaatggcat tatgaattta tctaaagaat atgttaattt gtatgataat
    21241 tatttgaaat atataaatga agtaaacaaa gagattagaa gtataaagaa catgaagaaa
    21301 cgtgttttaa aaaggggcga ttttgctccg gaaaatgaag tattagaatt ggaaggaaaa
    21361 acaatacgcg atatagatga tgtcgataca aaaaaaccaa aaaataaaat ctataaattt
    21421 tcaaatgggg atgtatatgt agggaaattc ttagagggaa agatggaggg gaaaggctca
    21481 tatacttttt ttgtagatga aggaacagtt atggagtaca taggagaatt taaagaagat
    21541 aaaaaaaatg gaaaaggaaa ctttatattt tcaaatggaa atgaatatat aggttatttt
    21601 gaggaagata tgatgagcgg tattggaaat atgttatata gtagtaaaga tgaatatatt
    21661 ggaacttgga aaaacggaaa aaaagatgga aaaggtattt ataagtggag tgatggatgt
    21721 atatatgcag gtgaatttaa aggtggaaag atggaagggt acggtatttg ctataatagt
    21781 aaaggggaag tactttatga aggagaatgg aagaataatt tgattcatgg taagggaact
    21841 tatatatggg aaaaaggtaa aaagtatata ggtgaattta tgcatggtaa aaaacatggg
    21901 caaggaactt tttatctaaa taatgaattg gtttatgaag ggacttggaa atttgataag
    21961 ccatctatat ttgatagaac attagatgaa atattttcct tgagactgta gctttatcta
    22021 attagtagat aaagcttttt tctaatactt actgtaaata ggtatgaatg atattgatta
    22081 gtatacacta tcaatattaa cttaataaat gcaatattaa aaaattttga ttgattttaa
    22141 gaaggagaga tatattgaag agattaattg ctttagtgtt agtagtactt attgttttat
    22201 tttatagtcc tgaattatta aaagaaggat atgatttaaa agaaaagttt tttcaaagtg
    22261 aaatatggaa tgaattttta gacaaaataa atataaatga taattctaag aaagataaaa
    22321 aagaaaaaag tcaaaattta caatcaaata atactgaagg ggagaactta ggagagtcag
    22381 atataaagaa ttttgaaaaa ataagcttag gagataattt aagctatgtt ttatcaagta
    22441 tcggcaaacc tggtagaatt gatattagtg aatatggttt tgattggtat gtatataatc
    22501 aatatggaaa agaatttgca atggttggac ttgaaaatga tgaagtagta gcactttata
    22561 gtaattctat taattcatgt gaaaatcaaa atataaaact aaatcaagat agacaaacag
    22621 tgagaactaa gattacacca ttaaaatata agagaaaagg aaataccaga tatataataa
    22681 actcagaaaa tcaatatgat ataataagta aggaaggtaa gtatattaca attttctacg
    22741 atattcatga agaaaataga gtttgttcat atttaataat tgataaaagc actgaagatg
    22801 agtttaaaaa tttatatcca gatgatagcg aagaacttaa aaaatgtttt gaacttgaag
    22861 ttatagacct tgtaaatagt gttagaaatc aaagagggtt aaactcattg agatacagtg
    22921 aacaggcaac tttgagttca agaaaacata gtgaagatat gagagataat aatttttttg
    22981 accatgtaaa taagaagaat gaaactccat ttgacagaat gaaaagagaa ggtatagtat
    23041 atacttcagc tggagaaaac atagcagcag gacagattaa tgcaatatat gcgcatgaag
    23101 cttggatgaa ctctgaagga catagaaaaa atattttggg gaactataat aatattggag
    23161 ttggagtaat atttggagga agctataaaa catattacac tcaaaatttt tataaataac
    23221 taaaatattt gaaaggaagt taaaaaatga catcatggaa aaaaaagaca gtatataagt
    23281 gcttaatagc agtagccttg ttttgtggaa tagtgttaat atctaatttt agtaaagttt
    23341 ctgctttgat gatggatact aatggaaatg ttttgattaa acatggttca agggaaaaaa
    23401 agctaatagc aattaccttt gatgatggtc ctcatcctaa agaaactagt caagttttag
    23461 atgtattaaa gaaatacaat gtaaaagcta ctttttttat agctggaaaa catgcaaaat
    23521 ggtataaaga accattagta agagcaagta aagaaggtca cgagatagga aatcatactt
    23581 tcaatcatcc tgatataagt aatttaagtt ctagtcagat agaagaggag attgttaagt
    23641 gtgaagacat tttgaaggaa gtaactggga aaaaacctac attatttaga ccaccattcg
    23701 gaagttatag agaaaaagac ttaatagaaa ttgcaaaaaa gcatgactat aaagttgttt
    23761 tatggacagg tgtagatgtg aaagattgga aaaatccagg agctaatagc attgcagata
    23821 aaataattaa taaagtacaa aatggagata taatactttt gcatgattat gcaactaatg
    23881 atacagttga agctttagat atgtttattc caaaaatgat tgaaaaaggt tttaaatttg
    23941 taacagtttc tgagttgata aaataatttt taggggggag atttacagta tgattcttat
    24001 gatagacaat tatgattctt ttgtatacaa cctagtccaa tatatagaag aattagggga
    24061 agcagttgta gtaaaaagaa ataatgaaat caaaataagt gacattgaag aactaaatcc
    24121 agaggtaatt gtgttatctc caggaccgtg ttctccaaaa gaagctggta tttgcataga
    24181 tattgttgag cattttaagg ggaaaaaacc tatactagga atttgtttag gtcaccaaac
    24241 tattggacat gtttttggag gagatattat aaaagcacaa cagcctgtgc atggaaaagt
    24301 atatagtatc aaccatacta ataaaggagt ttttagggga cttaaaaatc ctttaaatgt
    24361 cactaggtat cattcactaa tagttgactc caatacagtt ccaaaagaat tagaaattac
    24421 agcaattact gataaagggg aaataatggg aattaggcat aagaaatatt taatagaagg
    24481 agtacagttt catccagaag ctattttgtc agagtatggt catgaaatgc ttaaaaattt
    24541 tataacagaa gctagagaaa gggtacatat gtaatatgat aagagagata aatactaaat
    24601 taaattcttt tgagatattt actattttta gaaatgaaca tgacagtttt atattagata
    24661 gtgctatgga taaagaaaaa ttgggaagat attcttttat aagtagccaa ccatttaagg
    24721 tattaaagta taaagatact gatgaaaatc ctttagaagt tttaaaagaa gaactacata
    24781 agtatagggt tgtaaatgat actaatcttc catttgtagg aggtgctgtg ggatatttat
    24841 cttatgattt aggtaattac atagaaaatt tacctagaac tgctgtagat gatattgaga
    24901 tgcctgatat gtattttgga ttttataatc atgtaatagt tatagaccat cttgtgcaaa
    24961 aaacttatat agctactcca aacatagata tcgaattaga agaaaagata atagatgata
    25021 tagaacaaag aatattaaaa gaagagaaaa aaggtataga tagcatatgt tatgaagaaa
    25081 aagaagtaac acctataaga cttaaatcta attttacaaa ggaagaattt aaaaatgcag
    25141 ttcaaagtgt tagagagtac ataagacaag gagatatata tcaagctaat ttaacacaaa
    25201 ggttcagtgg agaaacagaa cttacaagct ttgaattgta tagggactta agaagattta
    25261 gcccagcacc atttggggca ttcttaaact ttgaagatgc acatatatta tcaaattctc
    25321 cagaaagatt tataagatgt gttaataaga gaatagaaac tagaccaata aaaggtactc
    25381 gtccaagagg gaaagataaa gaagaagatt taagacttca acaagaactt agaaatagtg
    25441 aaaaagatag agctgaacta cttatgatag tagatttaga aagaaatgat attgggagaa
    25501 tatctaaaac tggaagtgtt aaagttccag agctatttgt aattgaacca tatgcaaatg
    25561 taaatcactt agtttccaca gttgtaggtg agttaaaaga tgataaagat gctactgatg
    25621 taataaaagc aacttttcca ggggggtcta taacaggagc acctaagatt agagcaatgg
    25681 aaattataga cgaattagag ccaacgcaaa gaaatgtata tactggttca ataggttata
    25741 taggttttaa tggtgatatg gattttaata tagcaattag aacaataatc aaaaatgaca
    25801 aaaaagtcta ctttcaagtt ggaggaggaa tgacttggga ttctgaccca gatgaagaat
    25861 atcaagaaac acttgataaa gctaaatcca taatgaaggc tttgagggga tattatgaag
    25921 aataatgtag gttttgatag tagtttaagt aaatttggaa ttggtttgtt tgaaactatt
    25981 agagttaaaa atggaatagc agtagattta gatatccata tagaaagaat gctaagctca
    26041 ataaattgtt tggatttaga tataaactat aaaaaagatt tcttgataaa tgaaattgtt
    26101 acatatataa aaaaagaaaa tgtcattaat aaagcactta gaattactgt ttttgatgaa
    26161 ggttataata tatctattag ggatattcca tataatcaag agacgtatga gaaggggttt
    26221 aaattagcta tatctccaat agttaggggg aatagtttaa ttcatagaca taagactact
    26281 aattattttg aaaacatata taccaaaaat atcgcaaata agactggtta taatgatggt
    26341 atatttttaa attcagaagg tgtaatatta gaatgttcta tgagcaatat tttctttata
    26401 aaaggagaaa gagtgtacac tcctagtgat agattaccga tattaaatgg aataataaaa
    26461 aaaaggatta tagagatttg tgacgaatta catatagagt tgatagaaaa tgaaattaat
    26521 atatcagaaa ttagtagttt tgattttgtt tttgtcacaa atagtcttat gggcgctata
    26581 aaggttacag aaattgacaa aataaaattt aataaagaaa atgttgtttt tgataaaata
    26641 gtgaagttat tagaataaat taaaatatta acaatttgtt ggagacaaaa gatgataggt
    26701 tgtatttatg aagtatacaa tgaagagatg actaaaataa tagaaaacca aaacactttg
    26761 gctgtatatt tagtgggttc tagtaaagat gtagatttta ctctttatga tgtagtgata
    26821 aacgatattg atgtgttcgt ttttgtaaaa gaaggggaaa aacaagagag aatactgttt
    26881 aaaaaaaagg gaatagaatt tgatgtaaat tacttttcaa aagatggtgt aaaaaaatta
    26941 ttatcaaata gggaatattt ctttgtgaaa gaaatgaaag atgctaaagt actgtttgat
    27001 agggagaata tatcacatat tataaaagat ataagtagag atatttactt agaaggacct
    27061 agtaaaatgt cattagagga aaagtctttt ataaagcaag atattggagc aaaaatattg
    27121 aatttaaaaa ataaagaaaa atttgatgtt tttgaatatc attttttgac gaatttatat
    27181 ttaaaagata taataattgg gtactttaat ataaatgata aatgggtacc aaaagacaaa
    27241 aaattgctaa aagttttaaa aaaagaaaat aatgagcttt ttgaactagt gtcaaaagta
    27301 agtgaaaatt atgactatca aagactttta gatgtttata attatatttt taaagaaatt
    27361 gagacaaaag aggttataaa actaatcttc tagtcacaaa tcaatggggg gagtttaaat
    27421 gaataaagtt gataaagaaa agatacaaca tgcagtaaga gaaatactag aggcaattgg
    27481 ggaagaccca gatagagaag gtctaatcga aactccaaat agagttgcta ggatgtatga
    27541 agagatattc tctggtctta gtgaagaacc aagagaccat ttaaaagttc tttttgcaga
    27601 tgaaaaacat gaagagttag tattggtaaa agatattcca ttttattcat gttgtgagca
    27661 tcatctagtt cctttctttg gaaaagctca tattgcttat ttaccaaaag gaggaagact
    27721 tacagggctt tctaaacttg ctagagtaat agatacattg gcaaaaagac cacaattaca
    27781 agaaagaatc actaaaaatg ctgcagatat aataatggaa gaattacaac cttatggtgt
    27841 cttagtagta gtagaagctg aacatatgtg catgacaatg agaggcgtaa aaaaaccagg
    27901 ttcaaaaact gttacatcag cagtgagagg aatatttgaa aaggacattg cgtctagagc
    27961 cgaggctatg agtttgatta ctatgaagta gtaatttatt atagatataa tcatttattg
    28021 agataaaatt taatttaagg gagtgtcatt aattgtgttt gattatggta aaagaactta
    28081 tataatggga atattaaatg taactccaga tagtttttct gatggaggag attttaataa
    28141 cttggacata gcaatacaac atgcaaaaga tatggttgac caaggtgcag atataataga
    28201 cctaggagga gagtctacac gcccaggtca tagctatgta gactcagacg aagagctaag
    28261 aagagtaatc cctgtaataa aaaaacttaa acaggaattg gatataccaa tatcaataga
    28321 cacttataag gcagatgttg cagaagaagc attaaaatta ggtgtcacta tggtaaatga
    28381 tgtttggggt cttagaaaag ataaaaatat ggcttctgta ataggaaaat atgatgcaga
    28441 agtgtgtata atgcataatc aagatgggac aaactatgac aaagatataa tggagtcaat
    28501 aaaagacttt tttaaggtaa gtatagaaat ggcaatgagt tgtggtgtaa aaaaagaaaa
    28561 aatagtactt gacccaggtg taggatttgg caaggatttt gaacaaaata ttgaagtatt
    28621 gagaagatta aatgaattaa aagatttagg gtatcctatt ttgcttggga catccagaaa
    28681 atcagtaata ggaaaagtac ttccagttga gcctaaaaaa agattagaag gtacaatagc
    28741 tactactgtt cttggtataa gagatggcgt agatatagtt agagtacatg atgtttatga
    28801 aaatttaatg gcagctagaa tgactgatgc aatatataga aagtaggtat aatataatgg
    28861 ataaaatatt acttagtaat ctaggattct atggatatca tggtgttctt aaagaagaaa
    28921 attttctagg tcagaaattc tttgtcgaca tggaacttta tattgattca agagaagcag
    28981 gtcttagtga cgatataaat aaatctgtaa gctatgccga ggtttacaat gttgttaaag
    29041 atattactga aaataaacaa tttaatcttt tggaggcttt agcggaaaat attgctgaag
    29101 aagtcttgaa taaatttata cttataaatg gagttatggt cagagttaga aagcctgagg
    29161 ctcctgtaaa tggtatttat gactattttg gtgttgagat aaggagaaca agagatgaat
    29221 aaggcttatt tgggaatagg tactaacatg ggtgatagat ttgataattt atcaagagca
    29281 tgtgaacttt tgaaaaatag tgattctata tataaagtaa aagaatcaag cttatacgaa
    29341 acaaaaccat ggggatatac agaacaagca gattttctaa atatgtgtgt agaaatagaa
    29401 acagagtttg aaccttatga gttgttagaa tattgtcaag aaatagagag agaactacac
    29461 agagagagaa tagttcattg gggaccaaga actatagatg tagacgtact attttttaat
    29521 gatgtagttt ccactgatga aagactaaca atacctcatc caaggattca agatagagct
    29581 ttcgttttga taccacttat ggatttaaat gaagaactta taataaatga aaaaacaata
    29641 aaagaacatt taaacctcct atctgctgaa gaaagagaag aagtaaagga gcttgtaggt
    29701 tatgaaagaa aacctattta ataataattc aaagatagag ataaatacaa ataacaaaaa
    29761 gataactgta aaaaaagata aacttataat agctggacca tgtgcaatag agagttatga
    29821 gcaacttttg gagactgcta aatttgtaaa aagtcaggga gcaaatattt taagaggtgg
    29881 agcatacaag ccaagaacat ctccaaattc atttcaagga ttaaaaaaag aaggtcttga
    29941 aatattaaaa gcggttaaag acgaagttgg tatggctgtt attacagaac ttatggatgt
    30001 aagagatatg gacgaactgt atagtataag tgatataatt caaattgggt caagaaatat
    30061 gcaaaacttt actcttttaa gtgaagtggg aaaacaaaat aaacctgtta tgttaaaaag
    30121 aggaatagct tccacaatta ctgaatggat aggtgcttct gaatatatag caatagaagg
    30181 aaatagtaat attataatgt gtgagagagg tataagaacg tataatgatt atactagaaa
    30241 tacattagat ttagctgcag tacccattat tcaaaaggaa acaggtcttc ctgttgtggt
    30301 agacccaagc catgccactg gagttagata tttggtaaaa cctatgtcac tggcatcttt
    30361 tgcatgtgga gcagatggaa taatggtaga agtacatcct gacccagaaa atgctttgtc
    30421 agatggtgct caatctcttt gctttaatga atttgaagat ttaatgaaat caattaataa
    30481 ttattaaaag aggcaagagc ctctttttta ttcgtaatcg ttgctaaggt aaatttataa
    30541 gttataattt gatagttaga ttatttaata taaataaaga tttaatattg atagaattaa
    30601 tataaaaaat aaaaattttt tgattaataa gaaggatatc aataaaaaaa gtcgaattgt
    30661 gtatattgag tgattgacaa atttagaata tagtgtagaa tagaaatgtt aacacaaaaa
    30721 attacaccag aaggtgataa tatgaatgat ataaaagaca ttattgaaga aataaaagct
    30781 agatgcgata tagcaagtat aatatctgaa tatatgaata taaaacaatc tggagctaat
    30841 tacaaaggat tatgtccatt tcatggcgaa aagacacctt ctttttatat aaatacgtca
    30901 aaacagattt ataagtgttt tggttgtggt gaaggtggag acgtaataaa ttttgtcatg
    30961 aaaatggaaa atttggattt tatggatgct gtaaaaattt tagcaaataa atgcggtata
    31021 gaaattaaca ctaacatgaa tgaagaaact aggattaaaa tagaaaaatc taagaaattt
    31081 caagatattc atacggaagc agctagattt tatctttcaa acttattggg aagcaaaaat
    31141 cttgggtatg aatatttaag aataagagga ctagatgata aaattattaa gaaatttggt
    31201 ttaggatttt ctttggattc atggaattca cttatgaata ctctaatcag taaaggatat
    31261 aaaaaacaag acttattgga gtgcggtcta attgcaaaaa atagagatgg aactaattgc
    31321 tatgataaat ttagaaatag agttatgttt cctatattcg attatagagg aaatataata
    31381 ggatttgggg gaagagtatt agacgattct ttgccaaaat acttaaattc tccagacacg
    31441 ttaattttta ataaaaaaca gaacttatac ggattaaatt ttgctagaaa aaatttagaa
    31501 agtaaaacta tagtcttagt tgaaggatat atggatttaa tttcgcttta tcagtatggt
    31561 ataaaaaatg tagtagcaac ccttggaaca gccttgactg aacaacaagg cattttaata
    31621 aagagatatg cggacactgc tattatatct tatgattctg atgaagcagg aataaaagcg
    31681 actttaagag ctatagatat acttactaaa ttaggtatca atgtaaaagt gttagactta
    31741 aaagatgcta aagacccaga tgaatttgta agaaaatacg gacttagtga ttataaaaaa
    31801 gctatggatg tttctactca ctacataaaa tataaaatag atcatcttaa aaaagaattt
    31861 aacattcaaa aagatgaaga acgagtgaag ttcgcaaaag aagcttctaa aattataaaa
    31921 caattgacaa gtcctgttga aattgatttt tatacaaagt atttaagcaa tcaaatagat
    31981 attaatgtcg agtctataaa gagagaagtt tatggtaaaa attacaataa gccatataat
    32041 aacaaaaatc aaaagaaaat agaagaaaaa gtaattgaaa aagtcgaagt cagacaagac
    32101 ggaaaacaat tagttgaaga aactctaata aaaataatgt tagaagacaa aaaaattaga
    32161 gaaatagcgt tacttaaagt agaagaaagt gattttttat tgaaagaaag taaagaaatt
    32221 ttaaattaca tgattaaaaa tcaagaattg gacaaaataa ctattgacaa attgaaaagt
    32281 ttaaacatat ccgaagaata cttaaaagaa ttaaattcaa tttctttgaa tagtataaac
    32341 ttagagaaca caaaagaaat agaaggaata ataacaaata tcagaaaaaa ctcattagaa
    32401 gagcaaatta atagcttgtt gagagaacaa caagaattag aaaataataa tgatatgaaa
    32461 gaggtagatg gtagagttat ggaaattgct ttaaaaatag ttgagataaa taaaatttta
    32521 aagagcttgt agagaaagga ggtttgagtg tggaaaataa atcgaataaa aaagagttaa
    32581 aaaaggttac tgctaagaca ttaatagaaa aaggaaaaaa gcaaggttct ctgacacttg
    32641 cagagataat ggaagctttt tcggagactg aacttgataa ggatcaagta gaaaatcttt
    32701 atgagacttt aggaaatttg ggaatagaaa taacagaaac aaaaaactat aaagctgata
    32761 tagatttttc ggttgctgat gacgatttaa gtataggcca cttagatgaa gatgcagagg
    32821 caatttcaca tgatgactct tctgcaatag aaatagaaac tgtggattta tctttaccaa
    32881 aagggataag tatagatgac cctgtaagaa tgtacttaaa agaaatagga aaaattcctc
    32941 tacttaaacc acatgaagaa gtagaatttg ctagaaggat gcacgaaggt gatgagatag
    33001 caaaacaaag gttagttgaa gcaaacttaa gactagttgt aagtatagca aaaagatatg
    33061 taggaagagg tatgcttttc ttggatttaa tacaagaggg aaatttaggt cttataaaag
    33121 cagtcgaaaa atttgactat acaaaaggat ataagtttag tacttatgca acatggtgga
    33181 taagacaagc tataacacgt gctattgcag accaagctag aactataaga ataccagttc
    33241 atatggtaga gactataaat aagctaataa gagtatcaag acaactactt caagaacttg
    33301 gaagagatcc aaaaccagaa gaaattgcaa aagaaatgga aatgacagaa gataaagtaa
    33361 gagaaataat gaaaatagct caagaccctg tgtctttaga aacaccaata ggagaagaag
    33421 aagatagcca tttaggtgac tttattccag atgatgatgc tccagcccca gcagaggcag
    33481 cagcatattc tttattaaaa gaacaaatag aagatgtact tggttcatta aatgatagag
    33541 aacaaaaagt attaaagctt agatttggtc ttgaggatgg tagagccaga actcttgagg
    33601 aagttggaaa agagtttgat gtaactagag agagaataag acagatagaa gcaaaagcac
    33661 taagaaaact aagacatcca agtagatcaa aaaaacttag agattattta gactaaaaag
    33721 atacatcatt gatgtttttg tgataaatgc catgagataa gtgatatctc atggctattt
    33781 tgtagtaaag atttaatttt atttttgtag ttatttcagt ctaatatcta taacttggat
    33841 taaatcctat aaataaaggg aattgaaagg agaattatat ttgaaattaa cagatagatt
    33901 attgaaaata gcatcattag tgtcagatgg taaaaaaata gctgatatag ggacggacca
    33961 cggatacata ccagtatatt tgttaaaaga gggaaaagta ccatttgcag tgctagcgga
    34021 tgttaataaa ggacctcttg acaatgcaca taaagaagtc atacagaaca atcttttaga
    34081 taaagttgat ttacgattgg gttctggtat agagatactt gaaatagggg aagtagaaga
    34141 agtgataatt gctggtatgg gtggaatctt gataagtgag cttctcgagg ctaaaaaaga
    34201 agtagcacat aatgtagaaa aattaatatt acagccaatg caagcacaag aagaacttag
    34261 atattacctt ttaaacaacg gatatgaaat tttagaagaa gttttagtga gagaagattt
    34321 tagaatatat gagataattg ttgccaaata tacaggtaaa aatactataa tagaagatga
    34381 gatatattat gaggttggaa taaagctttt agaaaacaaa gattctcttt ttaatgattt
    34441 tatagaaaaa aagataaaga catatagttc tatagttaat aaattagaag ggaaaaatgg
    34501 ggaagctata gataagaaga gaaaggaaag cgaagttgca ataaaaaaac ttgaaaattt
    34561 aataaaataa ataggaggta tatatgctat taaagagtct aactagaaaa attgaaaaaa
    34621 agtatcctct aaacttagca gaggattggg ataatgtagg tcttatagtt ggagattttg
    34681 atatggatgt aaaaaaggta ttggtatctt tagaagctaa tgaagatgtt attgatgaag
    34741 ctatatccaa aaatatagat ttaattgtga cacatcatcc ttttatattt gggaaaatta
    34801 ataaaattaa tagtggagat ttaaaaggca gacttatcca aaaacttatt aaaaatgata
    34861 tatcacttta ttctatgcat acgaattttg atatagcatt tgatggtcta aatgactatt
    34921 ttatggaaat tatggaattt ggtaattcaa aggttttaga tataactaaa agcgaaaatt
    34981 tgtataaatt agcagtatac gttccacata actattcaga tgaacttaga aaggttttaa
    35041 gtaattcagg agcaggtcat atagggaatt atagtgattg tacgttttca atagaaggtg
    35101 aaggacaatt taagccatta gaaggctcta atccgtttct tggaagtgta aatgatatag
    35161 aaactgtaaa tgaagttaaa attgaaactg tagtaccaca gaagctttta ggtggggtaa
    35221 taagttctat gttagatgca catccttatg aagaagttgc atatgatttg tacaagttag
    35281 aaaataaagg tgagatattt ggactaggta gaataagtaa acttgataaa agtatgacat
    35341 tagaaagtct ttcaaggaaa ataaaagaga aattaaatat gaaacacata agagtagttg
    35401 gaaaccttag tacagacata acgaaagtag ccgttgtaac aggagctggt tcagaatttg
    35461 taaaaaaagc taagagacaa ggtgcagaag tactaattac gggtgatgta aagtatcatg
    35521 aggcacaaga tgcacttgat attggaatgt gtatagtgga ttgtggtcat tttgatacag
    35581 aagatatatt caaaaatgtt atgaagagat ttttagatga atttagtgaa attgaagtaa
    35641 taaaaagtaa tgtatatttg aatccattta gtactattta gcagtgtttg ataataaaaa
    35701 tactatataa aaaataaatt tgagtttaat tagggggata atgatgaaag tagcagtaat
    35761 ttttcatagt gtttgtggga gtacatattt attagctaga gaatataaag aagcgcttga
    35821 agaaatgaat atcgaggtag atatatttag agtaagtgac gaagttgcaa aaacacttcc
    35881 tcaatattat ttgataaatt ccaaagaata caaagacgaa tttgaaagta taaatgtaat
    35941 taaatctgga aaagaaatat tagattatga tgctatattt atgggttcac caacatatta
    36001 tggaaatgta tctggaccaa tgaagatgtt tatggatagt ttttctgata tttgggtagg
    36061 tactcctttg agtggtaaaa tatttggttg ttttgctaca gcaggttcac aacatggagg
    36121 aggagaatta gctttgcaag ctatgaatat atttgcccaa catatgggaa tgactctttt
    36181 gtcagttcca tgttcagtaa gaggaggtta tccagcgtat ggaatacttc atatagcagg
    36241 agataattct gatataagac caaatgatga tgccaagata ggtataagag attacctaaa
    36301 aagacttaac atataagaat atcatataaa aatacctaaa agcgactgta tatactttta
    36361 ggtattttta cttaattata aacgtgaagc tttaccagaa atatggtcaa tttctatttt
    36421 aacaacagtt gttaagttca tatccttctc tatgtacttc atgccttctt caaagaatcc
    36481 tttagaatat ttttttatta tttctacaag agcatttttc ttttcttcat tttctacagt
    36541 gcaagctttt ccaaaaacta tagcactaga ataagtagta ctaaatttat ctggaataac
    36601 actttcatta gcaactacta aaaaagaaac tttgttgttt ttcgatatat tatctaactt
    36661 atgcccattt cttgcacaat gaaagtaaat agaatcatta aagtatacat aatttacagc
    36721 aactccataa ggataaccat tttcagatat agtggaaaaa gtaccaaatt caccattttt
    36781 taatacttca acagtatctt cttttgtcat ttctcttttt ttcaatctca tttctttaaa
    36841 cataatatca tcctttctaa ttttgatgct taaattaaat tttagaacat ttataaagat
    36901 tatgctatgt tttttacaaa ttttaagaaa aaaatatagt ttaagataaa aggtagtgaa
    36961 agagaagcta tttggaggtt tatttatgat attattgtta gagtagtata aacaatacta
    37021 ataaatttaa tagctggttt cttgttattt aagatagtaa gcatgtctat aagaagggga
    37081 agttatcgag ggtagaagtt gttatttata actatattat aaagaaaata ttattatata
    37141 aaataataaa tagtaaagag gtatcaagtt gaatataata cttaatacct cttcaaaatt
    37201 aagttataaa agtgttgtgg gaattgtcct atacatggta aggagataat aaagaatgta
    37261 tctttaaagt ctcaactaaa cagcaaaatt tatccaacaa acatacaata aatgcttctt
    37321 tgtatctagg tggcttaaca gtaagtggcc acatgtgttt taaaattatg tccgtttctt
    37381 tttcatttat ttgaaaaaat aaagttgcat tttttaatgc ttctctaggg tgagtaaatc
    37441 catgtaatcc ttttctatca cctttgtaat gccaatcata aagaaaaaag tcgtgtagta
    37501 aagctcctct aataacactt tgaatatcaa cacttaagtg caatttttta cataatagat
    37561 aactataata tgctactgac aaactatgtt caagacatga aacattgcca tgatgaggaa
    37621 atgttctcat aagttgaact ttttcagaag aaagtatctc agaagcacat tcattaaaat
    37681 atttgatttc agcttttgac aacattatga aaatctccat tcttagtaat atataaatac
    37741 tatacttcaa cgataatatg aaatatttct tatcctacaa aatataatac ccaataatac
    37801 aagtaagata ccttttatgg tattttaaag taagagagat tttattaggc taaacaatga
    37861 aaaaattaaa agtaaagcta tagcaatata tagtattttt ccaagaggat tgccaaaatt
    37921 tatagtccat ccaataccaa accttttatt aaccattaat gatgggtcat ttggattatt
    37981 atacatgatg cctgctatcc aatacttctc atcttcttct ggagaataag atgagttgaa
    38041 ttttaaattt ggggaattaa tatatgttgc tattaaataa attataacta aaagcgttgt
    38101 aatattcata gcagttgata gttttgtttt tattgataaa agagtagtaa aaaactgtgt
    38161 tgtcattata atcatcaata ttaaaaacaa ataaccaatt ttatttaaat atttaatgtt
    38221 ttcaactcta ctttcttcaa ttctatttgt atctatctta atccttgact ttatggatcc
    38281 aatggaagtt atatataata aaaccattaa acaaaagtca gtaccgatta atttaaatac
    38341 attaagaaat gatttatcta taaaaacatc aggagaccca tttatgttcc aatgagtggc
    38401 aattgagtct ggcaattgat tataattaaa tgctataaaa atactcattc ccaaagtaaa
    38461 tagtatcggt attaaataga ttattttaaa tttttttatt attttatttt tcttatttat
    38521 aaaatccata tctactatta attttgaatc tatatctata tgacctaatt tagaataaat
    38581 ttctgattta agcttcttag cttttttatg tgtatgaata tatagtatac tttcatataa
    38641 taaaaaacct ataataccaa taaaatagga aagttccaat ttattaaatg taaagaccag
    38701 aataaaaact aatataaagt ctataataaa acctaaagat aatagttttt taaattcttt
    38761 atctaacttt ataaagtaat tataattttt atgctcttgg tctatagaaa ctccatagat
    38821 gtgtttttta cctacaaaat agggcataaa ataaaatgta gcatacatca aaattagaac
    38881 tggaaatatt gtaaatagac attcaatctt actcataata tttcacctca tacttgtcaa
    38941 ataaacttga acttagtgac aaaaattctt ctttactaat acctgataaa taagattttg
    39001 ccaccaataa ttccaagtca agtttatttt tttgacgagt tgtattatct attttcattg
    39061 gtagttgagc tacaatagca ccttttcttc tatcaatatt taagaatccc tcttctttta
    39121 attcattgta ggatttgttc actgtatgta aatttacacc aatattttca gccatacttc
    39181 ttacagatgg aagtgattca tttattttca attctcctga agctatagcc ttagttattt
    39241 cttctttaat ttgtacataa atagatttat cactattaaa atctaaattt aaaaccataa
    39301 aaaacctcca ttatgcttta taaatgaaaa cgattgttat atacatagta taacataaaa
    39361 aataataaca aacacaaaat ataagtttat attttgtaaa aattgtaatt taaaaaataa
    39421 cataattttt attaaaataa tacagatact atttatagat agaaataaaa attagaaagg
    39481 agaaaaaata tgttaacata tttaatgaaa agacaaatgc ataaaaaaga tgatggaatg
    39541 agtaaaccaa tgatgattac aggagctgta attacaggtg taggagcata tgctgtatat
    39601 aaaatggtaa aaagccataa atctaattct caaatggtca atacaaatta ttatggaaat
    39661 agcgattatg atgattataa atatgacgaa tttgattatg actgtgattg tagagacaca
    39721 aaacaagatt atgaatatga agaattaaaa aaaaaagtaa aagaatttaa ttcaagaaga
    39781 ataaattgtg aaaattgtcc acatgtttct caagaagaaa tgatgcaata tgtaaatagt
    39841 gttaaagatg taaaggaaga gattgattta catgaagatg ctaaaaaaga taatatttac
    39901 aaagaagatg aatacaaaaa atatgaagaa gaataagtaa tattttgtat aactgaacaa
    39961 atagtaatgt caaattattt aatatctagt tttaatatgt aagttgtaac ttaacttgaa
    40021 agaatcagta tcattcttta gatagttaag taacaactta cattttattt ttataaattt
    40081 taatagttct ttgtgactat tttgtaattt tagaaatatt ttagtatcca gggtaattgt
    40141 gtataataaa acttgtatag atagatagta tgaaattaag cagatagttt aaaattaaaa
    40201 tgaaaagaaa ggaaatagtt atgttaaagt tgttgatagt tgaagatgat agtactatat
    40261 cttttggaat aaagtatgct ttagaacaag aaggatttag tatagatata tcaaaggatt
    40321 tatctagtgg aaaagaaatg atttcttcta atgaatatag catgatactg ttggatgtta
    40381 cattgccaga tggaactggt tatgagcttt gtcaatatat aagaggattc tctcaggttc
    40441 ctataatatt cttgactgct tgtgatgaag aagtaaatat tgtaatgggt ctcgatatag
    40501 gtggagatga ttatataaca aaacctttta gaattagaga attaatatcc agaataaaag
    40561 ctgttttaag aagaaaaggt aatacttcag aagaaaataa gaaaatactt aaatttggag
    40621 atttaagtat atatacatta gaagctagag tatataaaaa tgataaagaa atatttctga
    40681 catcagttga atataaactt cttttaattc ttatacagaa taaaaatact gttttaagta
    40741 gaactcaaat attggaaaaa ctttgggatg taacttatga ttttgtaaac gacaatactc
    40801 taactgttta tataaaaagg cttagagaaa aaataggtga tgatttatgt gaaccaatct
    40861 acatagaaac tgtaaggggg attggttata aatggatagg aagtgaaaat agtgtttcta
    40921 tataatccag aagtgaagaa gtttttaagt aagtatgtta ctttgatgtt tattgttatc
    40981 ataatatcta ttggatttag cataatcaac gtgtccttaa ctaaagagat ggttgtaaga
    41041 aataatcagg ctataatagg aactttatca agcaagtatc caaacttgga aagtcagata
    41101 gtagacataa taactcaagg gaagtctatg gagaacatag attatggtca gaaaatatta
    41161 attaagtata attatgataa atctattagg ataaactctg agcctattat ttctaaatta
    41221 gttttagaga ctatcaaaat aaatataata ttagtatgta taatatttat tttgatattt
    41281 gtgttggttg ttagatattt taagtcaatt tacaatgatt tatcagatat gactaagtat
    41341 gtttactata gttcagaggg taagccattt gatatgaaaa acaagaatca agaagggcag
    41401 ataggtcttc taaaaactga actcttaaag atgacaacaa ttcttaatga aaaggttgaa
    41461 cttttaaaaa gagaaaagat atttttaaac aatacaattt ccgatatatc acatcaatta
    41521 aaaactccta tgacttcttt aataatgttg aatgatttat tatataatga tgtaccatac
    41581 gaagtaaaaa tcgatttttt aaataagata aaaaatcaat taaatagaat ggattggttg
    41641 attaaaagca tgcttaaatt atcaaaggta gaagcaaaag tgataaattt taaaaaagat
    41701 aaggtaaaat ttagtgaact catacataga gcaatgctat ctatgaaaat acctatggag
    41761 attaaaaatc aaaaactgac tatagaggga aatgataatg tatcttatat tggtgatatt
    41821 gattggtccg tggaagcatt agtgaatata atcaaaaatt gtgtagaaca tactcctgaa
    41881 tttggaaata taattataac ctataaagaa aatccattat tttctgaact aataataaaa
    41941 gatgagggtg aaggtataga taaaaaagat ataccacatg tatttaaacg attctataga
    42001 ggaagaagta gttcaaaaga agatagtgta ggaataggtc ttgcaatgtc aaaatctata
    42061 atagaaagcc aaaatggcga tatatatgta aacagcgaaa aaggtcaggg tacagaattt
    42121 catataatat ttcataaaat gtatgatagt gactaatctg taatatttaa ttcactttat
    42181 agtaatagcc aaaacctaat atatgaagta tagaatataa atattggagg tagttatgga
    42241 aattttaaga gtagaaaatc taactaaaag ttatggtaaa aatgaaacaa aagttgatgc
    42301 tataaaaaat gtaagtttat cagttgaaaa aggaacgttt atagcaataa caggtccaag
    42361 tggaagtggt aaaagtacct tattacattt attaggagga gttgacagac caactagtgg
    42421 taaggtttat ataaatgatg ttgatatata taatttaaaa actaaggatt tggcgatatt
    42481 tagaagaaga aatataggtc ttatatatca attttacaac ttaattccag tcttaaatgt
    42541 gaaggaaaac atattgcttc cagcagagct tgacaataga gatattgata gagaatattt
    42601 tgatgattta atgaaaacat taggtctaat tgatagagaa aagcaccttc caaaccaatt
    42661 atcaggaggt caacaacaga gaacctctat aggacgtgca ttaattaata gaccttctat
    42721 aatattagca gatgagccaa caggtaattt agatagtaaa aattcaaagg aaatattaga
    42781 attgttaaaa ttgtcagtaa aaaaatacaa ccagacacta ataatgataa ctcatgacaa
    42841 aaatatggca cttcaagctg atagaattat aagcatagaa gatggaatca taaaaagtga
    42901 tgaggtgatg tagatgaatt tatatacatt actaaccatt agatacttaa aacaaaataa
    42961 aagaagaacc attgtaacca taatcggtat aatactttca acagctttaa tttgtggtat
    43021 aggaaatata tttgaaagtt ttatggatta tcaaatgaga gaaactataa agaatgatgg
    43081 aagtttccat gttacatttt atgatgtaaa tagaaaaaat gtggaatatg taacgaaatc
    43141 agctgaaata gaaaaacatg catttaccaa acaacttgga tattctaaat tagaaaatag
    43201 tgaaaatgct atattaagta taaagcaata tgataaaaat gcaataaatg gatataaagt
    43261 ttctataaaa gaaggtagat ttccagctaa ggaaggagaa attgttctaa gtgaaagtat
    43321 tattaatctt atagatgata agttaaaaat aggagataag ataactttaa aagtagggga
    43381 tatgtttgac tcaagcaaga aaaagattga tagtatgaca tttcatgaag gtgattatat
    43441 agcaaatgaa aaagatagaa cttttaaagt tgttggtata attgaaaaac ctgggtttga
    43501 acgttataat ggaattgcaa cagctatagc ctactttaat cctatgaata attacaatga
    43561 tacaattaat gtatcagtag cagtaagaaa tcctaaagat gtgtataaga taagtaataa
    43621 aataagtaaa aatatatatt caaataaaga taaagaagca gtttctgata tgataaaata
    43681 caatgaacat ttattgaggt tacaaggtgc tagtaagtat tcaaatataa ataactctat
    43741 aaagtctata attatagcag taactatttt agtaatcata tgtacaattg ctacagtgta
    43801 caattccttt agtatttcta taaatgaacg aaaaaaacag tttgggatat taaattctat
    43861 aggtgctaca tctagtcaaa ttaaaagatt agtttttata gaaggtatta taattagttt
    43921 aattggtata ccaataggac taatatctgg aacagtagct atagacctat tgtttaaaat
    43981 tataaataag tattttactg aatcggttgt tactaagatg agtttgcaga tagtgtataa
    44041 tccaataata actattgtta gtataataat agttttgctt acaatcttta tatcaattct
    44101 actacctgca atatcggctt ctaatatttc tcaccttaat gtgattaaaa atactggaga
    44161 gtataaagtg ggcaaaatca aaagctcaag acttattaaa atgattttta aaactgaagg
    44221 agtacttgca tataaaaata ttaggagaaa tagaaaaaaa tttataataa ctcttttttc
    44281 tttaatggta agtataataa tatttatatc ttttagtggc tttacattac ttttactaag
    44341 aggtgaagaa attagaaatt ctcaaagaaa atatgatttg tatttaactg caaagaacac
    44401 agctaggtct gtagaggata ctataagtga actagaagat attgatggga taaaaaactt
    44461 tgaattggca acaggacaat atgtgtctat aagagtaagt gaaaataaaa taaataaatc
    44521 caaggaagat ttaatcagga aatattatca aaaatataag attggagata gttatgaata
    44581 tgatttcagt aataatgaat taatttttcc aggagatttt gcagtaaaaa acaaaataaa
    44641 taacttagta cagggctctt ttaataaaga gagagcaatt gaagaaaatg gtgttatatt
    44701 agttagaaaa agtgcatttg aagaaaatgg gaagaaaggt gtagtagaac ttaccaacta
    44761 taaagtagga gatacagtaa attgtgaata tcttgatgaa aatgccttaa gtaaaaaagt
    44821 aaaggtcaag attcttgcta taactgacga agaacgtcta ggtcttggtt atcaaaatat
    44881 ggggttacaa tttataactt atgatgaagt tgctaaaaat ttaaatttaa aattaagtaa
    44941 aagccttata tttattgatt caggaggaag cattaagact aagaaggaaa tagaagcttt
    45001 ggcaaataaa aataatttta acttttatga tgaaagtgca agcaatgaaa gtgaaaaaca
    45061 agatttaaag gttattaaaa tatttgtata tggttttata gtagtgatat ctttagtgag
    45121 tgttacaaat attctaaata cagtaagtac aagtataaat ctaagaaaaa gagaacttgc
    45181 agtaatacaa tctatagggg ttactcctaa aggatttagg aaaatgatat atttagaaag
    45241 ttttatatat ggtatactgt cattgttgtt tggaatacca attagtattg gtataattct
    45301 aattatgaat aaacttattt caggtgtaat tgaattttct ccgattatac cgtggacagc
    45361 catagttatc tgtatagtaa gtgtttttat aataactttt atagcagggt atataccaat
    45421 gagtaaatta aataaagaaa atataataga taatata
//
LOCUS       DAMJVI010000020        41413 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.020, whole genome shotgun sequence.
ACCESSION   DAMJVI010000020 DAMJVI010000000
VERSION     DAMJVI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.020"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            76..522
                     /locus_tag="PC545_001213"
     CDS             76..522
                     /locus_tag="PC545_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2455991.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNHIKKEFSDTEYKELVKNLHVVKDIKTAVEEE"
     gene            524..1618
                     /locus_tag="PC545_001214"
     CDS             524..1618
                     /locus_tag="PC545_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HDF2455992.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFSDIAKS
                     GVALITLGDVSIRPSFHKVAISLSDEDGVMKYKKIVDEIHNSGAKVSAQLFCSDYDVN
                     LIKDTMNMGITSHDEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLVGSFSSSIFNNRNDEYGRTCDNRSRFASEIISSIRDEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLLSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPTNNHPYFKEEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMVSMSRQLLADRNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTRSQFGCILD"
     gene            1706..2236
                     /locus_tag="PC545_001215"
     CDS             1706..2236
                     /locus_tag="PC545_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2455993.1"
                     /translation="MKSLVLYSSLTGNTKKIAYTIYDEIQEEKDIKDVNELVDYSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASKKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKAKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EASMFAKNMMIKASIV"
     gene            2563..2922
                     /locus_tag="PC545_001216"
                     /pseudo
     CDS             2563..2922
                     /locus_tag="PC545_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3173..4549)
                     /locus_tag="PC545_001217"
     CDS             complement(3173..4549)
                     /locus_tag="PC545_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455994.1"
                     /translation="MKYKIGFLTTSYFMSDKFKQALKELNDTCEITFIELKGENPFNN
                     LPNIYYEQVDNFDGFVCSGIVPYSELIINIKDIKVPLNFLKLDERDFYKYLFKLLNSQ
                     KDIDFTKSFMDFLREDNNYYDIYSLIDSNKCPYTIKDFNIPQPVYDFKKLNDKLLEIH
                     LDLLRDNKINLSFTRNYIISCELNKLGYDCIYMVPSTESILNTFKSLVNEITLQNLDK
                     NKSATCIVTVNSSEYINEDLIFKNDEIQNQIYNTILNSLSRHGFYGVQVKKNDMKIEI
                     HTTKEMLNNMTNNYTDFFISEDLKEIKYSLNIGWGIGNTNIHAEQNARQANGKSASLN
                     GNCTFIVNDTNDTIGPLYSNKNSNNIEDSSIANKVASFIPLSNTNVSKIMCMINDRNS
                     NNVSAEILSNYMNITLRSANRILSILYKAGIATIVNTKLDNQRGRPKKIYKVDFLSFL
                     NKMQKLNS"
     gene            4817..5989
                     /locus_tag="PC545_001218"
     CDS             4817..5989
                     /locus_tag="PC545_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2455995.1"
                     /translation="MNTYLNEISKFVDENREEIVSLWKEIVNIESYTHCKESVNKLAK
                     RLKFEFEKEGLDCELVDVGDNGSTLIGTLGSNINKKPIIFSGHMDTVFETGTFGENPF
                     KIIEGKAYGPGVLDMKGGIIISLYVIKALNKIGYKERPIKIVFSGDEEIGHKDSTGAD
                     VILREAKGGLCAFNMETGLVDNSLCVGRKGRIGCNIHVKGVETHAGNDFEGGRNAIEE
                     MANKILRIQKLTNLDVGTTVSVSVIKGGRVENSIPEDCSIKVDLRFEKVEEMENVKEQ
                     VREICKETYIEGTSTEVNFISEMMPFETTEDVMRFHTFVNEVSKENGFGELNAKRLGG
                     SSDASYLTIANVPAICSFGVRGEWNHTSREYAVVDSMFERVKLISTVILNLDKFEV"
     gene            6003..6650
                     /locus_tag="PC545_001219"
     CDS             6003..6650
                     /locus_tag="PC545_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2455996.1"
                     /translation="MDVTAGDNKVTWKGYLSLFVLIVLFSGVFKDSQGFLKALDFANL
                     TGSFGTIADGVDFMGKGGTGAKDGFLFALTLIPTVCFAVGLIDVVESMGGMKAASKIF
                     NPLLRPLLGIPGIAGIAFVSSFTSSDVASIMTKELFESGEITDDERTIFVAYQYAGSA
                     VILNTINTQAPLLPIALLALGPIILIEIFCKILGANLVRLIIGVRNKKNKAKEAV"
     gene            6652..7161
                     /locus_tag="PC545_001220"
     CDS             6652..7161
                     /locus_tag="PC545_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HDF2455997.1"
                     /translation="MSQQNTGNAIVNSKPKKTAVEIFMGGAKKGFYIGVEQILPAMIM
                     GYVIVQFLQLTGLVDILGNIFGPVMGIFGLPGESVLVLIAAFFAKAAGAATAANLFAQ
                     GLITAPQATILIMPCMLMGTLVGHYARIVLVANASTKYRMLLLAVPIFDAIVGMLIVR
                     VLLTVMGLM"
     gene            complement(7525..8637)
                     /locus_tag="PC545_001221"
     CDS             complement(7525..8637)
                     /locus_tag="PC545_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDF2455998.1"
                     /translation="MKISEVATPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKIECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     SEAASKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIEKVIEVDIRENSKQIIL"
     gene            8935..9201
                     /locus_tag="PC545_001222"
     CDS             8935..9201
                     /locus_tag="PC545_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HDF2455999.1"
                     /translation="MNLEPKKLIEQIGKEKDKKDIWIVGGGDIVKLFMKDNLIDDYYI
                     YILPIILGRGISLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            9446..11173
                     /locus_tag="PC545_001223"
     CDS             9446..11173
                     /locus_tag="PC545_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456000.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSILLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKDTENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            11173..12963
                     /locus_tag="PC545_001224"
     CDS             11173..12963
                     /locus_tag="PC545_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456001.1"
                     /translation="MKKSKGFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGVPGASVNFDYVTKILVILACIYFANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQSRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADKESE"
     gene            complement(13125..13433)
                     /locus_tag="PC545_001225"
     CDS             complement(13125..13433)
                     /locus_tag="PC545_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456002.1"
                     /translation="MFNKIYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(13695..14243)
                     /locus_tag="PC545_001226"
     CDS             complement(13695..14243)
                     /locus_tag="PC545_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456003.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILRKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(14346..15017)
                     /locus_tag="PC545_001227"
     CDS             complement(14346..15017)
                     /locus_tag="PC545_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456004.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEMIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIVEIFQIRSAIEGFCTFLITSQYKEA
                     RAVETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYRTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            15413..16318
                     /gene="garR"
                     /locus_tag="PC545_001228"
     CDS             15413..16318
                     /gene="garR"
                     /locus_tag="PC545_001228"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDF2456005.1"
                     /translation="MEGYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDINKSAVDEL
                     ISCGAKYASVLEMGQECDIVFTILPNGTIVQDILFGMDGLAKTLKEGSIVVDMSSVTP
                     TESILCANKLKDMGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SILIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            16454..17836
                     /locus_tag="PC545_001229"
     CDS             16454..17836
                     /locus_tag="PC545_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2456006.1"
                     /translation="MITGYGLLIAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFGISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            17907..19343
                     /locus_tag="PC545_001230"
     CDS             17907..19343
                     /locus_tag="PC545_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDF2456007.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPLETNLLKSEVERLSEKLFQGEIIMPFFKEGGRF
                     TIDNVHYVKEGEILVPAGMTEFAKDKSFGYKSSDIGEWCEEKTNGKYKSSDMIYISSK
                     ELRELNIEAITEKLKKAEGFNKIIVNATDYVDVKVFTIAFIRAVNSGKEFMFRSAAAI
                     TKILGGVSDKELLTKDELVSKGNTNGGIILVGSHVNKTTQQLEELKNCKYPIEFIEFN
                     QHLVLQENGLKNEVKRVIKVVEEKISRGKTVAVYTRRERFDLDTNDKDKQLMISVEIS
                     DAVTSIIGMLNVRPNFIIAKGGITSSDVGTKALRVKKATVMGQIKPGIPVWMTGKESK
                     FPNMPYIIFPGNVGEISTLRESVEILMG"
     gene            complement(19529..20383)
                     /locus_tag="PC545_001231"
     CDS             complement(19529..20383)
                     /locus_tag="PC545_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456008.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHTIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEIYFVLDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKLITQETIENKLESLVENRLDTLEDRFE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(20651..20974)
                     /locus_tag="PC545_001232"
     CDS             complement(20651..20974)
                     /locus_tag="PC545_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2456009.1"
                     /translation="MERINCNVINCSHNNSHICYADKIFINGEKAHNDRHTCCSSFLD
                     ESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNILVAPETDRVNLYIETCCS
                     SFECK"
     gene            21177..21806
                     /locus_tag="PC545_001233"
     CDS             21177..21806
                     /locus_tag="PC545_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456010.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDSGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
     gene            22149..22658
                     /gene="ruvC"
                     /locus_tag="PC545_001234"
     CDS             22149..22658
                     /gene="ruvC"
                     /locus_tag="PC545_001234"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDF2456011.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            22651..23253
                     /gene="ruvA"
                     /locus_tag="PC545_001235"
     CDS             22651..23253
                     /gene="ruvA"
                     /locus_tag="PC545_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDF2456012.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLSESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            23360..24379
                     /gene="ruvB"
                     /locus_tag="PC545_001236"
     CDS             23360..24379
                     /gene="ruvB"
                     /locus_tag="PC545_001236"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDF2456013.1"
                     /translation="MQGFEDENRIITSTMKMEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSSILDAEIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            24697..25722
                     /gene="queA"
                     /locus_tag="PC545_001237"
     CDS             24697..25722
                     /gene="queA"
                     /locus_tag="PC545_001237"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDF2456014.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLIGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLNKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGKNVICVGTTSCRTIESACNEYGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            25734..26219
                     /locus_tag="PC545_001238"
     CDS             25734..26219
                     /locus_tag="PC545_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456015.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKEQQNKEDEDKS
                     I"
     gene            26298..27419
                     /gene="tgt"
                     /locus_tag="PC545_001239"
     CDS             26298..27419
                     /gene="tgt"
                     /locus_tag="PC545_001239"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDF2456016.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHKFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPSDREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITNIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            27553..27846
                     /gene="yajC"
                     /locus_tag="PC545_001240"
     CDS             27553..27846
                     /gene="yajC"
                     /locus_tag="PC545_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDF2456017.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLSV
                     GDKVITIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            27904..28266
                     /locus_tag="PC545_001241"
     CDS             27904..28266
                     /locus_tag="PC545_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HDF2456018.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEIGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            28332..28475
                     /gene="scfA"
                     /locus_tag="PC545_001242"
     CDS             28332..28475
                     /gene="scfA"
                     /locus_tag="PC545_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HDF2456019.1"
                     /translation="MENKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            29102..30997
                     /locus_tag="PC545_001243"
     CDS             29102..30997
                     /locus_tag="PC545_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HDF2456020.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAEDSDNKLTATIKSTN
                     TVEVKRAKDSANVITLNVGDQHLDFSKVITSEEGTFEGYEKSYSDIDSKELHTITVKN
                     ADLQDISAEELFDGIRLTTLGREIVNKVKNGYALTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKPETISVSSKNHKLVRDLHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIEAEILRV
                     LGSNLSSKKIYIVGGESKVSKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            31459..32862
                     /locus_tag="PC545_001244"
     CDS             31459..32862
                     /locus_tag="PC545_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HDF2456021.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILINSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKAKTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGF
                     TGEADAISASPVAARDGVPIILTDGNSVGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFETNKIVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     ENGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     regulatory      33034..33119
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            33243..39206
                     /locus_tag="PC545_001245"
     CDS             33243..39206
                     /locus_tag="PC545_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding adhesion protein"
                     /protein_id="HDF2456022.1"
                     /translation="MKKAISCVLAVSMCSAPLNVFAEPILEGKLRAVEKSVTEKLRGS
                     LEVDLNFSLPIKNSESESMTNMQLRLKDDSNNSGIIKLGEKDSGTVNVGTDNIEYTIK
                     KLSASRSEIKNKDENISYYNIVFNNLPVGKYNVEVSGDGFKNKEIKDIDISSYSQRVL
                     LSNRASVDNKNKPLNYELFLMGDVNGDGKVDKSDYNKVLENIDSNKREFDLNRDGKVD
                     IVDLDYVQKNLGQSEDAKSLESIVSTNPIVDTSKVELKGNSDVDINGNVENLFSGENN
                     GVTISSKNDEISEEKPAVMDIALSEALNMEVINIKAPEGTAPAKGIVEVTDKDGNVQQ
                     IPYENKEYMKNLKDEEQQEEVNNEQEEVNQEEVNNKDENINQTDNNSNNKDENNDKQQ
                     ENSDKQEANSNTNQDKNNDKENSSMDKPNSDEDTPKEEGNISKDDNSTTSTYSRSALE
                     TYADVQNSDIVINLGKQVAVKKITIKITATTANDRNLAEISKVEFLNNVYKEIPKPEM
                     NIPKINKISTSTAVGNEKVSFYWNNEVNVSAYELILDKIDSKGKVLSTKKLQTSKNTI
                     EVRDIEAYGLYRVSIQSLNGDWSSGYKDATPENLDGVPENVDSNYKPIQFSPDSILEF
                     QVVPDTKPEPPEGINVKGKFKSLDVSWKNHKQAKDFDLYYKEKGSNGSWIKYNDKLIR
                     GTSSKIEGLKDNTTYEVRMTATNHLGTSGMSKTYIGTTESMDAPTTPNYRLINTPKEN
                     GGFGIPTNHIVDVVYPDGYDKSEYSDKNPFDKFNVVDGDFTTHWTFPTYNAAEGTNRG
                     PIITFDKSYTMDTFMATPRFDVSDKTINDFEVRYWDENGQLHNLGDVPFETKTDPVNN
                     NKYVMVRLKEPITATKIQVSVCSSYSYKSNISELKFYEYDSIEDDVRNLFMDGLQVEL
                     KEDVTQEKITELKDRLNTPDKASGEYHPYKTVIERELKLAQDLYNDRATLTDVTTVNQ
                     EINKLGKYKDKNGQYTVNSNNLGMQNDWQALGVAARAGDEITVYVGSKSGKTPKLIYT
                     QYYGESGAYKSGEINLNTGKNVIQLSKLHSLDIECGGSLYIRYAEDTPSGGDIKVRVA
                     GATKIPHLNLNGTINDKSPAGVSESKKKIKAYIEELAKYKDAVKGEPYYPGTNVGNSY
                     GYSEKTGVLNTTDIESDKVTLNVPATAVYQEISKGNSDLNTQVDRLYNSMLAWEQIMD
                     LVYSERGVFKTQDLDGNGTVDDNEFNLTKNDMAPKSRMNIKYQRMFIGAFMYASGLHV
                     GVEYGSVPGLMNGVPFQIDDSGKATGGNLFGWGIGHEIGHVTDIGKMTYSETSNNVLA
                     LLAQTFDDKTHSRLEGSTMDKIYEHVTSNSLGIPSNVFERLGMLWQLHLAYDDDFTGS
                     MLKNNSDADLSNDTFYAKISRKYRALSSSDPINSLPKDQMLVAMASSVVEKDLRDYFK
                     AWGVEITPELNSIMDSKNYEKESRKIQYVNDEARRKILNENIASMAKDTKVSASFSDG
                     IQSGSLVKSNKISIDLDVDKDKDKILGYEIIRSDGNYLDGENGTQVKYRTVGFVDAKG
                     NSKTKFTDNISPLNNRAFTYKVVAYDYHLNPTEEFEVGTVKLSDEGKINKSAWSFITN
                     TVSDGDVRTENDSHGPLQNPEIDNIKDNDASTTYKGKIISKAEWNKDPQKDPDINVEE
                     NPYIIVDMKETLPIVGLKYTKPETEARKFSLKGLFNFRKNTSTTYNPLTNYKIQVSND
                     KKNWEDVSQGTFEYGQDLVGGGKDQDNEARVTFNKDGKLWTYQARYVKLISNNKSNIE
                     VAELDIIGPPGDNIEIGIINNADETRTNGIGKLAEDYVYQSDDSSTSEDETGKIPAGS
                     IVITGEYSGNPAFNLPLLIDKNNKTISGEALLFAEVPEKGELGEISKGTWVYYMPKEN
                     FDSLSDKVKAELYRYNDLQGDTPVGQRFVSDTMYVPVGAKNYDELKTISLTDSNSKAR
                     KATRNSIDISNKKVITVSNKVKGNIVRDK"
     gene            39410..41290
                     /locus_tag="PC545_001246"
     CDS             39410..41290
                     /locus_tag="PC545_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp10"
                     /protein_id="HDF2456023.1"
                     /translation="MSKRKSFIRTIAVSTMAVAVTGSATCAYAAPVLQGTKTYEKVNT
                     IDISVDSVENIVYSFQASIKVQGEVEVVDNEQKEKITWSDNIKSQIKSGNANATCRVE
                     YNKSSNTTTLDIYVTSNEDLLDGNRLNIGRISVKKSGSNSNADYKVLGKGTNDKPALK
                     IVTYNNKTVDYENISSDEGLIFTLINESEVKPIGGTGSSKNDPEKYKVEKSEALEYLL
                     NNIRINYSIVSKETQESGSNVILKLGLAQKTTKGRKATINKYVEVTLPKSLEYIVENE
                     LSKPDELPPDNGNGGNNGGGSNSGGSSSGGSSGGGSSSDSTSNVTVKKLKGADRFETA
                     IKISQSGWTKSDTVVIVNGEDKSMVDGLTATPLASVKNSPILLSSNEKLPQKTVEELK
                     RLNPSKVIVIGGNNSMPNSVVEAIKAVNSKISVQRIGGDTRYQTSINIAKEIDKTNNV
                     SKLYIGAGNGEADSLSIASLAGKEKTPIVLTQKDGVDNEAEQFIKSNKVSNIYFIGGV
                     EKISNKAIEQVGKIANKDISNNRVAGQTRQETNAKVIDKFYSQSKLDGVVVANQDKLI
                     DALAVGPLAAKNNSPVILATNTLDKSQESSLKGKNSSKLFEVGGGIASSVIDKIKSLI
                     EK"
BASE COUNT        16306 a         4718 c         7210 g        13179 t
ORIGIN      
        1 ttgactatat taattgttta atataatata gttgatatag tcaaacatat tataattata
       61 gaagggaaat ttaagatgga ttttaaaaat ttacagtatg aaagtttgtc atatattatt
      121 tgtaacttgc aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc atataaactt
      181 acgaatggtc tatatttcta tttaatttat attaataaaa ataggaattg ttctttgaat
      241 gatgtttcta cagaatttga agttgataaa gctcatacaa caagaactat atctaggtta
      301 gaacaagatg gatacataga aaaaatccaa aaccctaatg atagtagagc ttttcagttg
      361 agagtaacag ataagggtga ggaagtatta ggggatataa aaaacatatt ttcaaagtgg
      421 gataatcaca taaaaaaaga gttctcggat acagaatata aggaactggt aaagaattta
      481 catgtggtaa aggatataaa aacagctgtg gaggaagaat aggatgtatt gtaatttatt
      541 aaaaccaatt aaaattggaa atttggaatt aaaaaataga gtatcttttg cacctacaag
      601 catgggtctt aaattggaag aaaaaattaa aaagttttct gatattgcta agtcaggagt
      661 agctctgatt actctaggag atgtatctat tagacctagt tttcataagg tagctataag
      721 cctatcggat gaagatggag tcatgaaata caagaaaatt gtagatgaaa tacacaattc
      781 tggtgcaaag gtatcagcac aactattttg ttcagattat gatgttaatt taattaaaga
      841 tactatgaat atgggaatta cttcacatga tgagataaaa aaaataatga atgacggagt
      901 aaaagattac ataacaaata tgcccaaaga agaaattaaa aatattataa atttatttaa
      961 agtaacagca ttgaatgcaa aaaaagcagg ttttgacatg attcaaattc atggtgatag
     1021 gcttgtagga agtttttcat caagtatatt taataataga aatgatgaat atggtagaac
     1081 gtgtgataat agaagtagat ttgcaagcga aataattagt agtatccgag atgaagttaa
     1141 ggacattcca atagactata aatttgcaat aagacaagaa aatcctcatt atggaaatgc
     1201 aggagtttta ctttcggaag ttgagtattt tgttaaaaaa tttgaatccc taggtgtaaa
     1261 cagttttcat gtaacacttg caaatcattc aaaattagaa gatactatac caaccaataa
     1321 tcatccttac tttaaagaag aaggctgttt tttatattta gctgatgaag taaagaaaca
     1381 cacaaatctt ccagtatgtg gggttggaaa attgtctagc ccagatttta tagaaagtat
     1441 tatttcaaat aatagagtag atatggtttc tatgtcaaga caattactag cagataggaa
     1501 ttggttacag aaagtaaaag atggaagagt agatgagatt aaaaagtgct gttattgtaa
     1561 taagaagtgt gcagatgctt tacaaacacg ctctcagttt ggatgtatac tagattagaa
     1621 tatccttata aaaataaata atttgttaag gttaatatat ttttggtgat ttagataagt
     1681 atttagataa agaggaggag acatcatgaa aagtttagtt ttatattcaa gtttaactgg
     1741 aaatactaaa aagattgctt atactatata tgatgaaatt caagaagaaa aagacattaa
     1801 agatgtaaat gaattagttg attatagtat tgactatgaa aattatgata tagtattctt
     1861 aggctattgg gtggacaagg gaatttgtga taaaaattca aaacaagtgc ttgaaaatat
     1921 tcacaataaa aagatagcac tatttggaac aatgggagca agtaaaaaag gaagttatgg
     1981 agctagtata atagaaaaga tagaatcaat tatcccaaaa gataatgaaa tattaggttc
     2041 attcatatgt caaggtaaga tagcagaagg attgaaagca aaatataaag agatgttaaa
     2101 actttctcct gacaacgaac atattcgcca acaattaaat aaccatgagg aatctcaaag
     2161 tcatccagat gaacaagaaa tatatgaagc cagtatgttt gcaaaaaata tgatgattaa
     2221 agctagtatt gtgtagtagt taaacaataa gttcatataa gatatagaat aaataattta
     2281 gtaaaaaagt atagcaaata atttgctatg ctttttttat gaaatcaaaa aaataataaa
     2341 aatacttgac tgtaaacctt gtttatacat tattttatag aaaataagat agttttaaat
     2401 ataaaaaaag atatattatt gaaaggagat attattgtat gaaaacttat aaaagataat
     2461 tagtttgatt ttaagatata ctaaatatac aattttatag agtgaaaata tttaagacta
     2521 tattatagtt ataaattaat agaatagaga ggtagtgttt aaattgcaat aacagtattt
     2581 ccaaagttgc cacctcaaat tcctatccaa tggaataatg gtgtagctaa ttatgtagat
     2641 aaaaaattta tttatgccta ccctgtcatt tgtgttgtat tcagaatcct tttacgtcca
     2701 tttatatgga gatggttaca aatacatgct ttttatagtg acactattgc agattattta
     2761 acgaatttta tgtgttttgt agcgttgtct atagaaatat tctctatact ttttatttac
     2821 aatattgcca gacatgttac aataattatt tttatagata tacttgtatt tattgcttta
     2881 ctgataatag gtatgtataa attatttaaa aaatacaatt aatttttaaa aattaagtaa
     2941 taagtgatag tgtaaaaagt agccactgca aattatggat tgtagtggct attttattat
     3001 ttgtatatga aattattttg tttttctaat aagttacgat atattatggt ttatttcact
     3061 gataaaaggt ttaggtattt taaagtaaaa agttgtatat tctaataaaa tttaaacatt
     3121 ttgattcttt aaaattagag aaaatacatt gttcttatta aaaaattaaa tattaagaat
     3181 ttaatttctg catcttattt agaaaagata agaaatcaac tttatatatt tttttgggcc
     3241 ttcctctttg attatcgagt tttgtattta caatagttgc aattcctgct ttatataaga
     3301 tactcaatat tctattagca cttctaagag ttatgttcat atagtttgat aatatttctg
     3361 ctgacacatt atttgaattt ctatcattaa tcatacacat gattttagat acatttgtat
     3421 tagatagagg tataaaagaa gcaactttat tggctattga gctatcttca atattattag
     3481 aatttttatt tgaatataaa gggcctattg tatcattagt atcatttact ataaaagtac
     3541 agtttccatt caaagaagca gatttaccat tagcttgacg agcattttgc tctgcatgaa
     3601 tatttgtatt tccaatgccc caacctatat ttaagctata tttaatttct tttaaatctt
     3661 ctgaaataaa aaaatctgta tagttattgg tcatattgtt taacatttct ttagtagtgt
     3721 gtatctctat tttcatatca ttttttttga cctgaactcc ataaaatcca tgtcttgata
     3781 gagaatttaa tatagtgtta tatatttgat tttgtatctc atcattttta aatatcaaat
     3841 cttcatttat gtactctgaa ctatttactg taactataca agttgcagat ttatttttgt
     3901 ctaagttttg taatgttatt tcattcacca gagatttaaa tgtattcagg attgattctg
     3961 tagatggaac catgtaaata caatcatatc caagtttatt aagttcacaa ctaatgatgt
     4021 aatttctagt aaaagaaagg ttaatcttat tatctcgcaa taagtctaaa tgtatttcca
     4081 ataatttatc atttaatttt ttaaagtcat atacaggttg agggatatta aaatctttaa
     4141 ttgtatatgg acatttatta gagtctatta atgaatatat atcataataa ttgttgtcct
     4201 ctcttaaaaa atccataaat gattttgtaa agtcaatatc tttttggcta tttaataatt
     4261 tgaataagta tttataaaaa tctctttcat caagctttaa aaaatttaat ggaactttta
     4321 tgtcttttat atttattata agttcactat atggaactat accactgcat acaaatccat
     4381 caaaattatc tacttgctca tagtatatat taggtaaatt attaaaagga ttctcccctt
     4441 tgagctcaat aaatgtaatt tcacaagtat catttaattc tttaagagct tgtttaaact
     4501 tatctgacat aaaataagat gttgtcaaaa aaccaatttt atatttcata aaacctcctt
     4561 taaaatttcg agagtatagt aactaatcaa tcaatttata acctcatttt ataatagtag
     4621 ataaaacgcg tcaatttatt ttacaaaagg aaaatttaaa gaaaaatcag aaaatataaa
     4681 aaagactgaa aaatatgttg actgtcaaga ataattgtgc tattttgtaa ttaaggacta
     4741 gtcttttaag atttcctaaa ataaacttag attttaggaa agaaatacat tttatttgaa
     4801 aataaaggag aagattatga atacatattt aaatgaaatt tctaagtttg ttgatgagaa
     4861 tagagaagaa atagtttcat tatggaaaga gattgttaat atagaaagct atacacattg
     4921 taaagaaagc gtaaataaat tggctaagag attaaagttt gagtttgaaa aagaaggttt
     4981 agattgcgag ttagtagatg ttggagataa tggtagtaca ttgataggta cacttggttc
     5041 taatataaat aaaaagccta taattttttc aggtcacatg gatactgtct ttgaaacagg
     5101 tacatttgga gagaatccat ttaaaataat agaaggaaaa gcatatggcc caggtgtttt
     5161 agatatgaag ggtggtataa taatttcctt atatgttatt aaagcattaa ataaaatagg
     5221 atacaaagaa agaccaataa aaatagtatt ttctggagat gaagagatag gacataagga
     5281 ttcaacaggt gcagatgtta tattgaggga agcaaaaggt ggtctttgtg cttttaatat
     5341 ggaaactgga ttagtagata atagtttgtg tgtaggtaga aaaggaagaa taggatgtaa
     5401 tatacatgta aaaggtgtag aaacacacgc aggtaatgat tttgaaggtg gaagaaatgc
     5461 tatagaagaa atggctaata agatactacg catacaaaaa ttgactaatt tagatgttgg
     5521 aactacagta agtgtaagtg tgattaaggg tggaagagtg gaaaattcta ttccagaaga
     5581 ttgtagtata aaagtagacc ttagatttga aaaagtagaa gaaatggaga atgttaaaga
     5641 acaagttaga gagatatgta aagaaactta catagaagga acttctacag aagttaattt
     5701 tataagtgaa atgatgccat ttgaaactac agaagatgtt atgagatttc atacatttgt
     5761 aaatgaagtg tcaaaagaaa atggatttgg agaattaaat gctaagagac ttggaggtag
     5821 ttcagatgct agttatttga ctattgcaaa cgttcccgca atctgttcat ttggtgtaag
     5881 aggggaatgg aaccacacat caagagaata tgcagtagta gattctatgt ttgagagggt
     5941 gaaattaatt tctacagtta ttttaaactt agataaattt gaggtataaa gggggatttt
     6001 atatggatgt aacagctgga gataataagg taacatggaa agggtattta tctttatttg
     6061 tattgatagt attattttca ggagtgttta aagatagtca aggcttcctt aaagcattag
     6121 attttgctaa tctgacaggt tcatttggta ctattgcaga tggtgtagac tttatgggta
     6181 aaggaggaac gggagcaaaa gatggtttct tatttgctct gactttgatt ccaactgtct
     6241 gttttgctgt aggtcttata gatgtagttg aatctatggg aggaatgaaa gcggcatcaa
     6301 agatatttaa tccattgctt agacctctgc ttggtatacc tggaatagca ggaatagcat
     6361 ttgtatcttc atttactagt tcagatgtag cctcaataat gacgaaagaa ttatttgaaa
     6421 gtggggaaat aacagatgat gaaagaacta tatttgtagc atatcaatat gctggttcag
     6481 ctgttatact gaacacaata aatactcaag ctccactttt accaatagca ttattggcat
     6541 taggtccaat aatccttata gagatattct gtaaaatact tggtgctaat ctagttagat
     6601 taatcatagg tgtaagaaat aaaaagaata aggctaagga ggctgtgtag tatgagtcaa
     6661 caaaatactg gaaatgcaat tgtaaatagt aaacctaaga aaactgctgt tgagatattt
     6721 atggggggag caaaaaaagg tttttacata ggtgttgaac agatattacc agccatgata
     6781 atgggttatg taatagtgca gtttttacaa ttaactggtt tagttgatat tttaggaaat
     6841 atttttggac cagtaatggg tatttttgga ctacctgggg aatctgtttt agttttgata
     6901 gcagcattct ttgctaaggc agcaggagca gctacagctg caaacttatt tgcacaaggt
     6961 ctaatcactg caccacaagc aaccatatta attatgccat gtatgttaat gggtactcta
     7021 gttggtcatt atgccagaat agtgcttgta gcaaatgcca gtactaagta tagaatgtta
     7081 ctgttggcag ttcctatatt tgatgcaata gtggggatgt taatagtaag agttttactc
     7141 actgttatgg gattaatgta aataacataa aattataacc taactataaa tgatatataa
     7201 gaattaatat atacaaggat taatgagtag taattgatat atagtaataa actaagatag
     7261 taattaatgt gtatattaat taaaaaaaga gagtatctca aagtgaactt tttagttcat
     7321 gagatactct ttttgtatga aatcaagtat attttcataa tttcaacaat gaaagagagg
     7381 cttatctcta ttttgagaca gcctcttctt ttaaatttat atttctagaa tttatgtttt
     7441 ttatttatcc aacattaaca ttagttatta gaaataattt taagaaaaat atactttcat
     7501 gttaatatca gaataaatct gtttttaaag gatgatttgt tttgaatttt cacgaatatc
     7561 tacttctatg actttttcta ttgtatctcc tctaaaacct aataaattac ttgtcaagtt
     7621 tgcagtggaa caagaatgat taggtataat tcttattcta tcaccaattt ttatatcagt
     7681 ttcaccattt actttaattt ttccaacttc ttctgaaagt gaatcaatag taagttctgg
     7741 atgtccacat accctaccat aaccaacaat actactattt ccatgtgcac ctttatcaag
     7801 tccaaggcac ttacttccac aatcaattaa atagtatcca ctgctatttt ttgagattac
     7861 agtgcacaca acagtaagag cacacatatc ttcagtagca caacctaaac ctatctgtat
     7921 agcatcatag tatacatagt ttccaggtct tacagcagtt atagttttag aagcagcttc
     7981 agattcaact gttggagtac taccagaagc gacaatttct atattaaatc cattatcagt
     8041 tagagctttg tatgctttct ccatagaaga atcttcttga acacaaacat ctggaacacc
     8101 ttctggagaa ctacaaccat acacttgacc tggatgcgaa cttataccaa caatctttat
     8161 gttttctagc ttagaaattt cagtagcaag ttcaaccact ttctctggtt caaccccaaa
     8221 acggtgtaag ccacaatcta caataataag tagcttgcac tctatttttg atttagaaaa
     8281 agcttcacta taagccttag cacattctat attatctgta gcacagatta aatttatttt
     8341 tgatgccata gtcacaattc tatttatatt tacttctccc atgtaaggat aagccaacat
     8401 aatatcttca aaacctgctt ttatcagagc ttcaccttca tcaagtgttc caactagaaa
     8461 accactagca cctgcatttt tttgaagtct cgctatgtaa gtacttttat gtgtcttaag
     8521 cataggccaa agttgtttat tattatttgt acaaatttgt tgaattttat ctatattctt
     8581 ttctaactgg tctaaatcta gcaaaaaatt tggagtagcg acttctgaaa ttttcatgtt
     8641 atttaccacc tttcaacaaa agtaaattaa ctataagtat tataattatt atattagtaa
     8701 tatcgtcttt ataatattaa tagcttataa ttatattata gaactagcgt taacagagag
     8761 caatatttat ttgtaataat tagaattttc aataagttag tgtggtttta aattatttat
     8821 ataatatatt tttaatttta tgcaaagttt atttaaatta gtttgaggat ttattaatag
     8881 ggtattattg aatgagtaaa aaagtgatgt ttacatagaa taatcacact ataaattaat
     8941 ttagaaccta aaaaattaat tgagcaaatt ggaaaagaga aggataaaaa agatatttgg
     9001 atagttggtg gaggcgatat agtaaaatta tttatgaaag ataacttaat tgatgattat
     9061 tatatatata tattgccaat aatattgggg agagggattt cactatttaa gtctggtata
     9121 gataagacta atttgaattt taagaaagct agtaatattg gagaacttgt aaaattggaa
     9181 tacagtaaaa aacaaaaata aattacatgc caatttgtta caatgttatg aaatgaagca
     9241 tttaaaagac ttttggctat atgaattgac attacttgtc aaatatgtta acatataagc
     9301 gacttgtcgg agggtgtatt aaaaagaata tgctggagaa gagagtgcgt taactctttt
     9361 ctcctttttt tataaaatta agcatttata aatttagtat tattacatga ttacactatc
     9421 tcaaaaatta agaaaggaga aaaatatgaa attaatttta agttatttaa agaattataa
     9481 gttattaata gtattaaaca tactggctat atttagtttt gcattagtcg agttggggat
     9541 tccaactata atagctaaaa taattgacaa tggtattgca aatcagaata tagcttacat
     9601 aaaacaaatg ggaatagtga ttgttattat ttctattata ggggttgtag gtagtatatt
     9661 gttgggttat tgttctgcta aggtatcaac aagtgtaacc agagatataa gaaatgacat
     9721 atttgagaaa tcacaagaat tttcacatac tgaatacaat aaatttggaa tttcttcaat
     9781 gattacaaga acaacgaatg atgcatttca aattcaacaa tttgttaata tactactaag
     9841 gactgcctta ttaactccag ttatgttcat tataagtata ataatgacaa ttagaacaag
     9901 tgtggaactt tctatagtat tggctatatc agtacctttt ataattattg gagtagcaat
     9961 aatagcaaaa gtatctcagg caatatcttc aaaacaacaa aaaggactag ataaattaaa
    10021 ccttatatcg agagaaaatt taactggaat aagagtaatt agagcttttg gaaatgatga
    10081 ttatgaaaca gagcgatttg aaaaaacaaa tacatattat gctaatgttt ctaagaaact
    10141 atttaagctt atgtcaatta ctcaaccagc attcttttta ctattaaata tagctgtttt
    10201 ggctgtattt tggatttcaa gtgagaagat aaatattgga gagcttcaag ttggacagtt
    10261 ggttgcattt ttggaatatc ttttccatgc catgttttca attatgcttt tttcaatggt
    10321 atttattatg tatccaagag cagaggtatc tgcaaataga ataaaagaac ttttaaatga
    10381 agaaccattg ataaaaaatc ctgaaaatgg aattaaggat actgaaaata agggtatcat
    10441 agaatttgat aatgtaactt ttacctatcc agatggagaa gcttctgtac ttaaggatat
    10501 ttcatttact gcaaaaacag gtgaaacagt tgcttttata ggaagtactg gtagtgggaa
    10561 aagtactcta attaacttga tacctagatt ttatgatgta acagaaggta gtattaagat
    10621 aaatggagta gatataagag aatatgattt aaaagcttta agaaaaaaga taggattcat
    10681 acctcaaaaa tcactgcttt ttactggtag catagcaaat aatattagat ttgggaaaca
    10741 taaggcagga gagtcagaat tagaatattc cgcaaaagtt gcacaagcat atgagtttat
    10801 atcaaaaaaa cctagaaagt ttgatgaatt gattagtgaa ggtggagcaa atgtatcagg
    10861 aggtcaaaaa caaagacttt ctattgcaag ggctattata agaagacctg aaatatacat
    10921 ttttgatgat agtttttctg cacttgactt taaaactgat gcaatacttc gtgctaaatt
    10981 gaagaaggaa acaaaagatg caattgtatt gattgttgcc cagagaatta gttctattat
    11041 tgatgctgat aagattatag tactcaatga aggacaagtt gtaggaatgg gaactcataa
    11101 agaattatta aaaaactgtg aaatatacta tgaaattgca acatctcagt taaaaaagga
    11161 ggaattagaa taatgaaaaa gtctaaaggt tttaagaaaa caattggtag acttcttcca
    11221 tttgtaaaga aatataaatt ttcctttatt atagctatta tatgtataat ttcagcagct
    11281 actatgaatg cacttgcacc taaaactgaa ggattgataa taacacaatt gactaaagat
    11341 gtaattagta tcgcaaaagg agtaccaggg gcttccgtaa attttgatta tgtaacaaaa
    11401 atattggtga ttttagcatg tatatatttt gccaatgcaa tttttacgta tacttcaagt
    11461 tttctactta ccaatgctat tcaaaacaca atgagagatt taagaaatga agtcgaaaat
    11521 aaaataagaa gattgcctat tagttatttt gatagtaata gttttggaga tgtattaagt
    11581 cgtatatcaa atgatgtaga tacaatatca aatgcattgc agcagagttt tatgcaaatt
    11641 gtaaactcaa tcttagtcat aattttggca ctttcaatga tgtttacaat taatatatat
    11701 atggctctta tagctctttt tataattcca attagttatt ttgtatctaa atttgtagta
    11761 aaaaaatctc aaagtagatt ttcattgcaa caaaatgctc ttggaaaatt aaatgggaaa
    11821 gtacaagaaa tgtatactgg atttaatgaa ataaaactat atggtaaaga agaagattct
    11881 ataaaagaat ttaaaaaagt aaatcaagaa ctttgtgaaa atggatttaa ggcacaattt
    11941 atatcaagta tgatgaatcc tatggtttca ttagtaactt actttggaat tgcagctgtg
    12001 gcagttgtcg gttctatata tgcagtaagt ggaggaataa cagtaggaaa tttacaagca
    12061 tttgtgcgtt atatatggca gataaatcag ccactttcac aaatgactca gttatctaca
    12121 gtaatacaat cttcttttgc agctatagaa cgtgtatttg aaattttaga cgaagaagag
    12181 gaaatacctg atgttgaaaa tccagttaag atagaaaatg taaaaggaaa tgttacattt
    12241 gagcatgtta attttggata tggagaaaat tcaactttaa tagaagattt aaatgcagaa
    12301 gtaaaaagtg gtcagatggt agctattgtt ggacctactg gagcaggtaa aacaacatta
    12361 ataaacttat tgatgaggtt ttatgatgta aagaaaggtg ctattaaaat tgatggagta
    12421 gatataagag atatgaaacg taaagatttg cgttcaatgt ttggtatggt gcttcaagat
    12481 acatggttat ttaatggaac catttttgaa aatatagaat atggtagatt tggagctact
    12541 aaagaagaaa ttatacaagc agcaaaagta gcaaatgttc atcactttat aaccactctt
    12601 ccagatggat acaatatgtt tttaaatgaa gaagcatcta atatatctct aggggaaaaa
    12661 cagcttttaa ccatagctag agcttttata tcagaccctt caatattgat acttgatgaa
    12721 gctacgagtt ctgttgatac taggctggaa cttatgcttc aaaaagcaat gagaaatctt
    12781 atgaatggta gaactagttt tgttatagca catagacttt ctacaattcg caatgctgac
    12841 ttgattttag ttatgaataa tggaagtatt atagagcagg ggaatcatga tgaattaatg
    12901 gaaaagggtg gattttatga gaagctttat aatagccaat ttgcagacaa agaatcagaa
    12961 tagataacat aaacttttgt ataaaatttt gtatcaaatt caaatattaa tcatataatt
    13021 aaaaagagta tcagtaagtt gtttttttat agatttactg atactctttg attttaagac
    13081 caaaggtact tatgattggt acttataatt cacaactagt cttgttacat ctgataacca
    13141 gaattacagt acttttcttt tttacctttt tgttctctaa catataacaa ttcgcttaac
    13201 ttatcaatgt aataaccatc atcacttatg cgaattaaac tagtcagata atatccatct
    13261 ttgcttctag gaatcttttt atttattgct aaatcaatag ctgtctttgc tgcttcaaaa
    13321 tgctttaaat gtgtatgacc ttcttcaaaa ctctttctag tattataaac tacataacct
    13381 tttttaactt gaaaaactat aaattctttc ttttcatata ttttattaaa cattcaatca
    13441 gcccttctca agatagtttt atagcataac attagaagct tctaaaaaat tttacaaaga
    13501 acataaatat tttataaatt ataaatctaa aactataatt tataaagata actttctgat
    13561 tatttatatg ataattcata tagattaaaa gtaaattatc tttaggttaa acaaaagtaa
    13621 aataaaagac tagttataat atttatatga aaaagtacaa aatatagtat atgaatatag
    13681 aaaatttaaa ttatttattt acagttttta tttccataag tatatttacc ttcagatacc
    13741 ttgtcatcat ggaaatatat tctttcacaa cttttatctc cccatagaat gttttttgtt
    13801 attttatggg acgtatctcc tgtaaatact tcagctacag tagtatctgt aacatcacag
    13861 taaagagaag cgaatttttt aaaccaagga ttttcctcaa atcttaaaag ccaggaagct
    13921 ccataggggc aatgtaactc ttcaaattca ggaacccatc ctagaaatgg aacatcagtt
    13981 gtgctaataa tattgtctgc aattaattca tctccatttt catgagctct ctctctcata
    14041 gcaatagctc tttcttcaga cctttcaaat aaaacttttc ttaatatttc gctagtttta
    14101 tcttcaccaa actcttcaaa taacactttg gaaaaataaa aatataaatc accaaaatgt
    14161 ttgcatgcca tacgaacttc ttcaatagca taatctttgt caatagtttc attagttaca
    14221 acatcttcgt tcaaaaaaat cactccttag aataagtatt agttacaata taattataga
    14281 atatttttta taaattagga aattaatttg agatagatat atttttaata aatgaaagta
    14341 taatgctaaa ataacttatt gttacaaaaa tcttctaaat taatgtattt aggagtttcc
    14401 atatgaatga gagtagttct gtaaatatct tcaatacatc catttgcaat attattaaaa
    14461 atatctgtgt gctcttttaa tgttgtttca agccttcctt catgtgacaa tgaatcgaga
    14521 gctaattttt ttattctgta catataattg ttaaacattt tgtcaaattc agtattttga
    14581 gcatatgaaa ctatagttgt gtgaaataat gtatcgtatt ctgcaaatga gtttaaattt
    14641 ttatcaccta ataaaatatc tttttgttta tctaaaagat gttttaattt acttatagtt
    14701 tctactgctc tagcttcttt gtattgactt gtaattaaaa aagtacaaaa tccttctatt
    14761 gcagagcgta tttggaatat ctctacaatt tcatttgctg taatttgatg gattctaaat
    14821 cctttgcttg ggattatatc tataaatcca tcttgactaa gtctttgaag agcatctctt
    14881 ataggtgttc tagagatacc tatttcacta gcaagttttg tttcagaata tatttcattt
    14941 tctatcattt ctccagatag tatcaattct tttaagtagt catatgcttg ttcttgtaat
    15001 cttttaaatg tagccatact taataatacc cccctaaaca atataaaaat acaaaaactt
    15061 atataatttt aagcatacaa catagtgaaa ataaggtcaa tcttttgcaa tttaaattgc
    15121 agataagatt aaaaaatata aaaaattatt gaagtgtgaa gcatgattaa cattaaaggc
    15181 agattaatta aaactaattg aaattagtta aaatacaaaa ctgtaaaagt agtatattat
    15241 atttgaaaac aatagtatgt attaaaactt atataggatg actaaatata ataaatgact
    15301 ttaaaaatta ctatttattg ttaaaataaa tacttttata ttttatattg accggtatac
    15361 tggtatgtag tatactctaa aacataaaaa acgttttcaa aaatgtgaag atatggaggg
    15421 ttataaaatg aaattgggat ttattggatt aggaataatg ggaaagccaa tggcaaaaaa
    15481 tttattaaaa gatggatgta atcttttggt gtatgacatt aataaatctg ctgtggatga
    15541 acttatatca tgtggagcaa aatacgcaag tgttttagaa atgggtcaag aatgtgatat
    15601 tgtatttaca atccttccaa atggaactat tgtgcaggat atattgtttg ggatggatgg
    15661 attggcaaaa actttaaaag aaggaagtat agtagtcgat atgagttcag ttactcctac
    15721 agaatccata ttgtgtgcaa ataaattaaa agatatgggc ttagaattta tagactcacc
    15781 agtaagtgga ggggagccaa aagcgattga tggtacactt gcttttatgg ctggtggtaa
    15841 ggaagaaatt tataagaaag tagaaccatt ttttaatata atgggaagct catctatttt
    15901 aattggagat aatggtagtg gaagtgttac aaaacttaca aaccaagtaa ttgttaattt
    15961 gacgatagca gcagtttctg aggcatttgt acttgcagca aaagcaggtg cagacccaga
    16021 aaaagtatat aaggcaataa gaggaggcct agctggtagt acaatattgg atgcgaagat
    16081 accaatgatt atgaatcgtg attttaaacc aggtgggaaa atatctatta atttgaaaga
    16141 tattaagaat gtaatgcaaa cagcacacaa tttagatgta ccactaccta tgacaagtca
    16201 attattagaa attatgcaga cattaaaagt acatggacat ttggaagatg accatagtgg
    16261 aatagcacag tattttgaaa agttggcagg tgttgaaata aaaaaacaca ataattaata
    16321 tttaaatata tcaatataat taaatacata ttattaggat tagattatat aaatacagcg
    16381 aggttaagtt atcatagtaa aagaaaaata aaattgataa ataataaagt agaatgataa
    16441 aggggtaaag attatgatta caggatatgg acttttgata gcatttgtta tatctatagg
    16501 aattttactt gtatcgatta ttaaatttaa agtaaatccg tttttggcat tactgataac
    16561 atctataatt acaggcttta tggttagaat gcctataaat gaaatatcaa ccacaatatc
    16621 gactggattt ggaaatacat taggtagtat aggtattgta attggacttg gaataatatt
    16681 tggaaatatt ctttcagaat caagagcaac agaatctatt gcaaaggggt tgttagctaa
    16741 gactggagaa aaaaacagtg ctttagcagt aactacagca ggttttttaa tttcaattcc
    16801 agtttttatg gatgcagctt ttgttataat gatgcctata attaagtatg tttcgagagt
    16861 aactaagaaa tctctaatgg tttttgtatg tgccttagga gttgggacta tagtaggtca
    16921 tgcacttgta atacctacac caggaccttt agcagttgca gcaaatgtaa atgcaaatgt
    16981 aggtagtttt atactttatt ctataattgt tgcttttcca gcagctctta taggtggatg
    17041 gatttatgga aaacgttttg aaaaatatcc agcttatgct attgatgaga atgatagaga
    17101 aaaaaattta gagcagggaa aagatagcat aaaaattgaa gatgatgatt cttctaaagt
    17161 accaggtttt ggaatttcta tgttttcttt attgttccca atacttctta tacttatatc
    17221 aaatgtattt agtatgttct tagaaaaagg ttctacaatg agtggagtac ttgcatttat
    17281 aggagataaa aacatagcaa tacttcttgg aatattggtg gcaataggat ttttaaagaa
    17341 atacataaat aaacctatgg gagatgtagt tattgaagct gctgattcag cagggctaat
    17401 attattgata acagggtcag gtggtgcgtt tggcagtgtt ataaatgcaa gtggtatagg
    17461 taacttctta gttgatacta tgtctggtct tagtatatca gttgtagtgc ttggattctt
    17521 attaagtgcc ttgcttagaa tttctcaagg ttctgctact gtagccttag taacaacatc
    17581 atcaatttta ggacctacaa ttttggcaac tggaatgtca ccagttttag tagggcttgc
    17641 tatatgtgct ggaggagttg gtttctccct tccaaatgat tctggattct gggtactttc
    17701 aagatttagt ggattatcag ttaaagatac tttaaactct tggacaattg gaggaaccat
    17761 tgctggagtt gtagcattta taatggtttt attgcttagt gttataaatg gaattatacc
    17821 acttccaggt ctataaataa caaactattg tttatatcat ataaattaca tttagactat
    17881 acaaattaca taaaggggcg ttttaaatga gagaagtaaa accaattagc atagatatac
    17941 ttaatacttt taaacaagta gatgaggaca gattaaataa acttcttgca gatgagctta
    18001 agcatttaga tagaaaaatt gttgtattag atgatgaccc aacaggtgta caaacagttc
    18061 atgatatttc tgtttatact gattgggata aggatagtat ggaacaaggt tttaatgaga
    18121 aaaatagtat gttttttata cttaccaatt caaggggatt tactgttgct caaactacaa
    18181 aagcacataa agaaatttca aagaatattg tggatgtatc aaagaaagtt aataaggatt
    18241 ttataatcat aagtcgtagt gattctacta tgagaggaca ttatcctctt gaaactaatt
    18301 tattaaaaag cgaagtagag agattatcgg agaagttatt tcagggtgaa ataataatgc
    18361 cattttttaa agaaggtggt aggtttacta tagataatgt acattatgtt aaagaaggag
    18421 aaatacttgt accagcagga atgactgaat ttgcaaaaga taaatctttt ggatataaat
    18481 catctgatat aggtgagtgg tgtgaagaaa agacaaatgg aaagtacaaa tctagtgata
    18541 tgatatatat ttcgtcaaaa gaacttagag agctaaatat tgaagctata acagaaaaac
    18601 ttaaaaaagc tgaaggattc aataaaatta ttgtaaatgc aaccgattat gtagatgtaa
    18661 aggtatttac aattgcgttt atacgagcag taaatagtgg taaggaattt atgtttagaa
    18721 gcgctgctgc cataacaaaa atacttggcg gagtgagtga caaggaacta cttactaagg
    18781 atgaattagt atcaaaaggt aataccaatg gaggaattat acttgttggt tcacatgtga
    18841 ataagactac ccaacagtta gaagaactta aaaattgtaa atatccgata gaatttatag
    18901 aatttaatca acatttagtt cttcaagaaa atggtctaaa aaatgaggta aaaagagtta
    18961 taaaagttgt agaggaaaaa ataagcaggg gtaaaactgt tgctgtttat actagacgag
    19021 agcgttttga cttggatacg aatgataaag ataaacagct tatgatttct gtagaaatat
    19081 cagatgcagt taccagtata attggtatgc ttaatgtaag accaaatttt attatagcta
    19141 agggtggaat tacttctagt gatgttggta ctaaagcact aagagtaaaa aaagctacag
    19201 taatgggaca gataaaacct ggtataccag tatggatgac aggcaaagaa agcaaatttc
    19261 ctaatatgcc atatataatt ttccctggaa atgtgggaga gattagcaca cttagagaat
    19321 cagttgagat attgatgggg tagtatatta gtgtattgaa gtttaaacgc ttaaatttac
    19381 tgagttaaaa ttaaaataaa ctaaaattat aaattaagat taaaaataat agtatattaa
    19441 ttagggttta gagtatttta caaaaaatat tctaaacctt aatcttatgt attagtattt
    19501 ttattattta acttatgtgt ttattttatt atttatcttt tttaattgat ttaatatttt
    19561 ctttgatttt atttaaaata tctgtatgct tattagagct tatgttgggg aatgctttga
    19621 ttatacgtct atgaagtaat ttgtggttag acagcaaagt attaagttta ttttttagct
    19681 catcaatacg atttttagca tagcgataat tttctttttc tttaagtatt tcaaacctat
    19741 cttccaaagt atccagtcta ttttcaacta aagattcaag tttgttttct atagtttctt
    19801 gtgtaataag tttgtagtat tctttcttat cttttaattc cttaattata agattgattt
    19861 gttttaactt accatttaat tgtaaaattg tatggactgt gataactaaa tctaaaacaa
    19921 aataaatttc tatagatata gcaaatataa atagcacatt tttagagata gagttaataa
    19981 agtcaaccac aataggatgt actataagca ttacaatcaa tgataataca ccaaacataa
    20041 gtgagttttc cagacaaact cttccgtgaa tattaaattt atgtttagaa taatcccacc
    20101 aagtcatatt aaatattttt tctaaaataa agctcgtaca atactccatt atgctagtta
    20161 taattatact aaacaaaaac aacaaaggaa tagtgtgtac aaaaggagtc aaggtagcaa
    20221 ctattgctaa tgctccaaat ccatatatag ggcaaaatgg accatttaaa aaacctctat
    20281 ttacaaattg tttttgtaaa actgaacaat atgtagtttc acaacaccat cctataaagc
    20341 tataaattac aaaataaaga aattgaatat aaaatgtact cataagcacc tcatttctag
    20401 ttagtataac actatatact cgatatttta tttttatgat taatttacta aattcaattt
    20461 acatattgat tataccaatt tatagaaatt tatcaaattt aattacagtt agaacattat
    20521 aaacatggtt ataataatga aattttattt aaaataaata agggtgtcaa gtataatcaa
    20581 actaatagtt gtagatactc tacactatgt tagtttaatt acatttgaca cccttatatt
    20641 tatactatga ctatttacat tcgaaactag agcaacatgt ttcaatataa aggtttactc
    20701 tatcagtttc aggagcaacc aatatattat caagtgtaca taagctacca tctttgttgt
    20761 atttacaatt ttttacagta caaactaatt cctcacaatt tctattatta acgttgctag
    20821 ataaattact ataaatagat tcatctaaaa atgaactaca acaagtatgt ctgtcattgt
    20881 gagctttttc accatttata aaaattttat ctgcataaca tatatggctg ttgttatgtg
    20941 aacagttaat tacattacag tttattcttt ccaaagttat tacctcctaa acgattattt
    21001 attataaatt atgtccatat aattacaaga atataaccat taagagaaaa ctacctttta
    21061 gaaaatatct attcttagag taatttgaaa tataaatata ttttcttcta aaatttacct
    21121 aaattgtata aaaacttaca gcttggagaa aataatagaa aaaggaggcg taatcaatgt
    21181 ttgaaaagaa gaaaaaagaa ttatttcctt ggaaaatatc tgccttaata ctggttggag
    21241 tatttgtatt aagtagcgga gtttatatag ggacaagatt aaaacacaat ggagttcaaa
    21301 ctggtgaagt tgtaagtact agtgcgaata tgaatgaaaa gtcaaaagaa gcttttggat
    21361 taaataaaaa ttgtgaagtt tggcttcaaa gtgaaggcga aaatgaaact aactataaga
    21421 gaacacttac aatggcagga atagttccag agtcattatt agataagacg aagtcagaaa
    21481 tagtatcata ttttaaaaaa gaatatccaa ctaaaaaaat aaagagtatg gataaaaatg
    21541 aaatcatatt ggtagaatct aaatatagtg ataataaaga tgtacaagca atggatgtta
    21601 ctataaaagg taaatatact attgaaaatg ataatggaaa tatagcacta tataaatatg
    21661 atgatagtgg aagcaaaaca ttagtagaaa aaacaaaaat aagagtagat tcacttccta
    21721 aaacagttca agatgaaatc aaaaaaggtg tagttatgga tactgaagaa gaggcctatt
    21781 caagattaga agactttgca agttaatagt aaattaatat tattttaagt taaatactaa
    21841 gtaagttaaa tactaagtaa attaataata aataatttaa tattaaatag attagtatta
    21901 aatagattag tattaaaatt gtagtcacat ttaattccta taaaaataca ataatttatt
    21961 tgtaataaat ataaaagtta tattgaggta aaatcttgct ttgatataac ttttttattt
    22021 gattttatat tttacataga gcattttatg atttgttaat attatttatg atataatgat
    22081 ttagaacata agtttttatg agaaatacat tttggtattt cttttatttt tagggggaat
    22141 tttaaaaaat gataatattg gggattgacc caggtatagc catagttgga tatggtataa
    22201 ttgaatacaa aaatagcaag tttaaagtaa tcgattatgg agcagttaca acacctgccc
    22261 atatgaatat atcgagaaga ttggaacttg tgtataaagg aattgataca atagtaaaga
    22321 attacaatat agatgaagtt ggaatggaag aattattctt taacaagaat gtaaaaacag
    22381 ctataacagt tgcacaagct agaggtgtta ctatgcttgc atgtgctcat aatgggaagc
    22441 ctgtatatga atacactcca cttcaagtaa aacaaggtgt agttggatat gggagagcag
    22501 ataaggcaca agttcaacag atggtaactt catttttaag tctaaaaaaa gttccaaaac
    22561 cagatgatgt tgcagatgct ttagctgtgg ctatttgcca tgctcattca aacaaacttg
    22621 aaaaaactct aaagaatata ggtggtaagt atgtatagtt atataaaagg tacagtagaa
    22681 gaaatttata tagacagtat agttgttgaa aataatggga taggatataa aataaatgta
    22741 tcatccaata caataatgaa tttacaagtg ggagaagcca caaaaatata tactaaatta
    22801 attgtaagag aagatgatat gagtctttgt ggttttgtaa gtagggaaga acttaagatg
    22861 tttgaattac taacatctgt atctaaaata ggacctaaag tggcgctttc aatactatca
    22921 tttgcatcac cagcacagtt aggagcatat attttaagtg aagacattgg aaaattatcc
    22981 aaagctcctg gtgttggtaa aaagacagca gaaagaatcg tcttagagct taaggataag
    23041 gtagacaaaa ataacataga gtttgaacca acattattat cacaaaaacc taccttgata
    23101 tcacaagatg agtctgtaga tgcacttgta gctttaggct atactttgtc ggaatcaaaa
    23161 gaggcagttc aaaaatgtaa aaaagatggt atgaatacag aagctattat aaagaaagca
    23221 cttacatata taatgagtaa atccttgaaa tagtatttgg attttataat atttggattt
    23281 taaaacaata tttaaggagt ttatgtaaat ttgaaaatat ataaagtaga ttttcaaaag
    23341 tatgagagga ggggtataaa tgcaaggttt tgaagatgaa aataggataa taacttctac
    23401 aatgaagatg gaagatatag atatagaaaa tagtttaaga ccaaaaactt tagaagatta
    23461 tttgggtcaa gaaaaatcca aagaacaact tagtatattt atagaagcag ctaaatctag
    23521 aaatgagcag ttagaccatg tgcttttata tggaccacca ggtcttggaa aaactacact
    23581 agcaagtata atagcaaatg agatgggggt aaatttaaga attacgtcag gacctgcaat
    23641 agaaagagct ggagatttgg cagccatact tacgaattta aatgaaaatg atgttttatt
    23701 tatagatgag attcatagaa tcaatagaag tgttgaagaa gttttatatc cagcaatgga
    23761 ggatttttgc ctagatataa taatagggaa agggccatct gctagaagca taagacttga
    23821 ccttcctaaa tttacactta ttggagctac tacaagagct ggtatgctta caaatccact
    23881 tagagataga tttggtgtta tttgtaaatt ggactattat acagttgatg aactttcaaa
    23941 aatagtactt aggtcttcaa gcatcttaga tgcagagata cagtctaatg gagcattaga
    24001 acttgcaaaa cgttcaagag ggactccaag gatagcaaat agattactaa aaagagttag
    24061 agattttgca caagtcagag ctgatggaaa aataactgat aaggtggcaa aagatgctct
    24121 ggaactttta ggagtcgata gtttgggtct cgattttgta gatgagaagc ttcttatgac
    24181 tataatagaa aaatttagag gaggaccagt gggtttagat acattggcag cttctatcgg
    24241 agaagataga aatacaatag aagatgtata tgagccatat ttgcttcaat taggatttat
    24301 aaatagaggt cctagaggaa gagtagctat gccattagca tatgaacact taaaaattcc
    24361 atatcctaat gaaaaataaa aatagattat acgagtattt ataaaataaa agagtcatta
    24421 aattaaaata tttctaaaat ggctaaaaat tcaaacatat ccacaaaatg gtaattatat
    24481 ctgtagatac tgtggataaa cacgataaaa attgtgtgta ttgtatctat atattgtgga
    24541 taagacatgt atatcgtgga aaaatatcta tatattgtgg atatggtgtc tgttaaaaat
    24601 agcaatttct tgatagatta tggtaatatt gtggaaaata agttgttttt ttgatattat
    24661 tgtagctgga gaaattttaa gagaggaagt ttaacattga aaacaagtga ttttaaattt
    24721 gacttaccac aggaacttat agctcaagtt cctatagaag atagagctag ctcaagattg
    24781 atggtattag ataaagagac aggtaatatt gaacataaag tatttagaga tataatagaa
    24841 tatttaaatc caggagattg cttagtatta aataatacta gagttatacc agctagatta
    24901 ataggagaga agcttgaaac tggaggtaaa atagagtttt tattattaaa aagaacagag
    24961 gaagatactt ggcaggcttt agtaaaacca ggaaaaagag caaaagtagg gactaaattt
    25021 tcttttggaa atggaaagct aattggagaa gttgtagatt tatcagatga aggttcaaga
    25081 attataaaat tccattatga tggaattttt gaagaaatat tggatgaact tggaaatatg
    25141 ccactacctc catatatcac tgctagacta gatgaaaaag aaagatacca gactgtttat
    25201 tcaaagcata atggttcagc agcagcacct acagcaggat tacattttac agaagaattg
    25261 ttaaataaaa taaaagaaaa aggtgtagat atagcctttg taactctaca tgttggctta
    25321 ggaactttta gaccagtaaa agtagaggat gttttaaatc acaagatgca ttcagaatat
    25381 tatatggtta gccaagaagc agctgacaaa ataaatagag caaaggaaaa tggaaaaaat
    25441 gtaatatgtg ttggtactac cagttgtaga actatagaat cagcttgtaa tgagtatgga
    25501 aagatgaaag aaactagtgg atggacagaa atatttatat atccaggtta taaatttaaa
    25561 gttttagata aacttattac taattttcac ttaccagagt ctacactaat aatgttagta
    25621 agtgctattt gtggaaagga taatgtttta aatgcatata atgaggcagt aaaagaaaga
    25681 tatagattct tcagttttgg agatgctatg attataaagt agggaggtaa tggatgcata
    25741 gtttacaaaa acaaaaaaag aataacccta tattaaagat tgcttttata tttgaacgtg
    25801 ttttagcagt agtagttcta atagcagtat ttttaggaac tatagatgtt ctaagactta
    25861 tgtggggagc atatatagtt gattttcaaa atccagtaca atacagtcag cttaatgatt
    25921 ttttagcaca aatattactt ttagtaatag gtgttgaact tgtagtaatg ctatctcttc
    25981 atataccagg agcatttatt gaggcattac tttacgcaat tgctagaaaa atgttgctac
    26041 tgcctaaaaa tgaaggtatg atagatgttt taataggagt tatagctata gcaggactat
    26101 ttgctataaa aaagttttta gtaacaaaag acaagagtgc tcttaatata atgagtattc
    26161 atgatgaaga agcaattaag gaacaacaga acaaagaaga cgaagataaa tctatttaga
    26221 caataaatta tatgaatagc ttattacaaa tatatcttaa gaaataatga gaaataaata
    26281 tgattaagga gaactttatg tacgcagtaa gatacgaatt gataaagact tgtaagcaga
    26341 gtggtgcaag gttaggaaga ttacacactc cacatgggat tattgaaaca ccaatattca
    26401 tgccagttgg aactcaagca actgtaaaat ctatgacacc agaagaatta aaagagattg
    26461 gttcacaaat aatacttagt aacacatacc atttatatat gagaccaggt catgaactta
    26521 taaaaagagc tggtggtctt cataagttta tgaattggga taaaccaata cttacagata
    26581 gtggagggtt ccaggtattt agtctagggc ctttaagaaa gataaaagaa gaaggtgtag
    26641 aatttaggtc acacttagat ggttcaaaac attttctaac tccagaaaaa gctatggaaa
    26701 tacaaaatgc attgggttca gacataatga tggcatttga tgagtgtgca ccatacccat
    26761 cagatagaga atatgtaaaa aattctttag aaagaacaac aagatggtta aaaagatgta
    26821 aagatgccca taataatacc gacaagcaag ctttatttgg tataatacaa ggtgggatgt
    26881 ataaagactt aagggaacaa tcagcgaaag aaataactaa tattgattta ccaggatatg
    26941 ctattggtgg acttagtgtt ggagaaccaa aaccacttat gtatgacgtg ttagagcata
    27001 caactccact tatgccaaag gataaaccaa gatacctaat gggagttggt agtccagatg
    27061 acttagtaga aggtgttata agaggagtag acatgtttga ttgtgttcta cctactcgta
    27121 tcgctagaaa tggaactgca atgactagcc aaggtaaagt agtagttaga aatgcaactt
    27181 atgctgaaga ctttactcca cttgaccctg aatgtgattg ttatgcttgt aaaaactatt
    27241 caagagctta tataagacac cttataaaag caaatgaaat actgggtgca agattaataa
    27301 ctactcataa tcttcacttt ttattaaatt taatgaaaca aataagacaa gcgataatgg
    27361 aagatagatt acttgatttt agaaatgaat tttttgcaaa atatggatat gaaatatagg
    27421 ttttgcacta ttaattataa tcattttaat atataatatt caatagtact caaagataga
    27481 gaaagtaaat tgttatataa acatttatta tgatttacaa aagagtataa tttacaaagg
    27541 agtgaaaaat taatgccaca gcaacaaata attatgagta taggtctatg ggtagtagta
    27601 atcgcaatct tttatttcct aatgattaga cctcaaaaga aaaaagacaa acaattaaaa
    27661 gaaatgagaa gcagcctaag tgtaggagac aaggtcatta caataggtgg aatagtagct
    27721 aatgtagcta aagttgaaga tgatgttgta atattagaat taggaccaaa tagaactaaa
    27781 gttccatttg aaaaatgggc tataggaact gttaaatcta aaaaagaaga aatagaagaa
    27841 gactaaatta gtaatttaaa agccttttct cgaatatata ttaatttgag aggaggtttt
    27901 tttatgggaa aatctaatta tatttttaaa ggtctaggat atgcatatat aataactttg
    27961 gctgttttat tagtgtataa cttatttttg acatttacag atattggtgg agataatata
    28021 actatggtct catcatttat aactactatt tctgcagcta taggagggtt ttacacttca
    28081 aaacacatga aagaaaaagg acttatgtat gggcttttag taggactttt gtacatagta
    28141 tgtatttttt tgactgtgtt tttagcacaa gaaaagtttg tatttgaaat agggatgatt
    28201 tataagttat tgctaatatc agcagcagga ggcattggag gcgtattggg tgtcaacttt
    28261 aaatagagtt tatattaaag tagttttatg atataatcaa taggaaacaa ttagaaatgg
    28321 aggcgttgaa aatggaaaat aaacatgtaa aaactttatc tcaagctact ttaaagcaaa
    28381 gtgctgcaaa aggtggatgt ggagagtgtc aaacttcttg tcaatcagct tgtaaaactt
    28441 cttgtacagt tgcaaatcaa gagtgtgaaa gatagaaaat tattgattta gttgtttgta
    28501 agagggtatc tcaaagtgat tcttttatca ttatgaggta tcctctttta ttttcaatta
    28561 ttatataaaa atatatattc tatttatgta gaagtaaatt tgatttctta tcgtttaatt
    28621 atgcaaaaat atctaatcat gtaagagcat ttattttaaa gtagctactc ttatatttta
    28681 ttttttatca attacatgta ttagaataat ttttaatttt aaactctaat ttatgatgat
    28741 ttttataata tgttatatat tttaatatat ggagatagat attaatttag tgatatatat
    28801 ttaagtaata agagtaaagc aaaaattagt gcatagtagt atataaaaat ggagtttgaa
    28861 tacttttgtt agtatatgat tgtttagtag aaaatcacta taaaatagga tttctatctt
    28921 tgagcataat tatagagagc ctaagtaggg ggaagggttt caagaagata ttattagtgt
    28981 ttgtgttagt aaattatgta aacacataaa atatattgaa aaataatatt atatataata
    29041 ttatttatgt gtgggaaata cgaatataaa aaataaaaaa taatattcgg aggacataaa
    29101 tatgttaagc aacaaaaaga gaagtatggc aatagtaatg gcaggtgcta cagttatgag
    29161 tgcagcagca ccaatattcg cagacaatac cgtgacagaa aatgttgata aaaattacac
    29221 agtaagtgcg aaagattctg ctaaactaat cgaagaggtt agaaaagcat tagaagttaa
    29281 gtttgaagat acaaaagcag gagcaaatgt aaatgataga gtttacgata taaaagtaga
    29341 caatgtaaac ttaactaatg caactcaatt acaaaataaa ataaactctt taacagaagg
    29401 gcaaagttta aaagttacta ttcaagataa agggcatcaa gtgttaggtg gaaaagtagt
    29461 tgactataaa attgaaaact acaaaactgc tcaagaaata gtagatgcag ttaatgctta
    29521 caatgcaact ttagcagaag attctgataa caagttaaca gcaactataa aatctacaaa
    29581 tacagttgaa gtaaaaagag caaaagattc agcaaatgtt attacattaa atgtaggaga
    29641 ccaacattta gatttctcaa aagtgataac atcagaagaa ggtacttttg aaggttatga
    29701 aaagagttat agtgacatag attcaaaaga attacatacg ataacagtta aaaacgcaga
    29761 tttacaagac atatcagcag aagaattatt tgatggaatt agattaacta cattaggaag
    29821 agaaatagtt aataaagtta aaaatggata tgctttaaca tttgaaaatg aagcaatcct
    29881 tactcaagaa caagaagata gtgatgataa agataaacca gaaaaatcaa gctttgatat
    29941 agttttaagc aaggcaaatg aaaagccaga aacaataagt gtttcaagta aaaaccataa
    30001 gttagttaga gacttacaca aagtattaac tgatgtaaaa gatggaaaag aattaaaagt
    30061 agaagtttta tctggtgatt caagatttac aacagcagta gaagttagta aagagagatt
    30121 taaagatgga gaagcagaag ctataatttt agttggagaa gatgctatag ttgatggatt
    30181 agcatcagca ccacttgcct ctcaaaaaaa tgcaccaata ttattatcta aaaaagattc
    30241 actaccatca gaaatagaag ctgaaatatt aagagtactt ggaagtaatc tatcttctaa
    30301 gaaaatatat atagtaggtg gagaatctaa agtatcaaaa gaaactgaag aaaaactttc
    30361 taaattaggt gtaagtaaag ttgagagagt ttctggagaa gatagatttg aaacttcttt
    30421 agaaatagca aaacaattaa aagatacatt taagactgct tttgtagtag gtggaaatgg
    30481 agaagctgat gctatgagta tatcagctag agctgctcaa tttggtgctc caataatagt
    30541 tacaggtaac gaattagatg caaatgctga aaaattatta aaaggaaaag aattagaaat
    30601 agtgggtgga gaaaattctg tatcaaaaga agttgaagac aaattagttg atatagattt
    30661 aaataataaa gttgaaagat tagctggaga aaatagaaaa gatactaatg ctaaagtaat
    30721 caataaatac tatgcaggtg caactaaagc atatgtagca aaagatggtt atgtaggtgg
    30781 aaatggacaa ttagttgatg cacttacagc agcaccactt gcagctagtt caaaggctcc
    30841 aatagtatta actacagaag aactttctaa atcacaagaa gaagtagttg agttaagact
    30901 taagaatgct actaagttag tacaaatagg tgaaggaata gctaaaaacg ctattgaaaa
    30961 aatagcagaa aaaataaact tatttactaa gaactaataa gatataggaa ttaatattaa
    31021 aagtttattt agataatata tgattataca atgcgttaat atgtaagaca tagtgtagat
    31081 attataaaat atacaaataa caagataaaa taataaaaat aaggtgtctc atttagaatt
    31141 agattgagat accttatttt attttttgat tataatgttg agaaattcat attgcaaaaa
    31201 ttattataaa aagatacttc ttataatgat taaacatatt actaacataa attattttga
    31261 acttataacg taattaaata aaataaatta taatgttttg aatttataga gaataaaaac
    31321 tttaggtata ttgtttgagt tgtatggata actatggtat catatataaa atatggtaac
    31381 aaaattgtaa cttttgatta aaaataagta tgagtactaa taaaaaatat gaaaagtaca
    31441 ttgggggtag aaaataatat gaagaatagt aaaaagatat tagctatagg acttacacta
    31501 tttttagtaa tggtaaatac tcctatggta aatgcgttga catcagttga gcaaataaaa
    31561 gggaatgaca gatatgagac agcagcaaaa atagcagata agcaaaacta caatacagca
    31621 atactaatca actcagataa tagcttagca gatggtctaa gtgcaagtgg tttagctgga
    31681 gccttaaatg cgcctatttt gatgacaaaa caaaaccaga ttccaaacac aactatggaa
    31741 agattaaaca aagcaaagac tgtgtatata ataggttcag aatcaacaat aagtaaaaat
    31801 gttgagaatc agttactatc caaaaagaag gttgtacaaa gaatatttgg tgaaaataga
    31861 tttgatacaa gtataaaaat agctgaaaaa atcaaagaaa ttaagccaat agataaagta
    31921 attatagcca atggatttac aggagaagca gatgcgataa gtgcatcacc agtagctgct
    31981 agagatggag tacctataat acttacagat ggaaatagtg tgggatttga cacgacaggc
    32041 ttaaagagtt atgcgcttgg ttctagtgaa ataataagcg acgaattagt taagagcaca
    32101 aattctatta gattgggtgg cacagataga tttgaaacta ataagatagt tatacaagag
    32161 ttttataaaa atagtaaaga attttatcta agtaaaggct tacaattaac agacgctttg
    32221 gctgcctcta caatagcaaa aaatgctcct gtagttttag ttgaaaatgg cagtaacaag
    32281 agtattttaa gtggtgcaga caaattgact gtactaggag gcataaatca gaatgttatc
    32341 aagcaatgta taaatcaagc ttcaccaaat caacaaggtt tatattacaa tccaaatgat
    32401 agagcattta aagagagaat aaagggtaaa gtatatgctc ttacaaagca gtatagaaaa
    32461 gagaatggag taagagcgtt atctgtagca agcagattag agggtctagc aaatgattgg
    32521 tctaatttaa tggcaaataa gaagacttta tctcatacga taaatggaaa aaattcatat
    32581 tctacttttt tgaaatactt agattggagt gagattaaac ctggatatat agcagttcaa
    32641 ggtgaaaata taattaaata taaaattcca gataaacctg tatatacaaa tagagatgca
    32701 gatgatatag gaaactttat ttttaatgag tggaagacaa atccagaaga aggaactaat
    32761 atgttgcata aagggtatga aataatggga tttggtatag ctataacagg agataagaat
    32821 ctatatgcta cacatgaatt ttatggaaga tataaagaat aagaaaatat acacaaaatt
    32881 atgcttgtaa ggaagctata taaataatta aattataatg gaaaatagca tttatattaa
    32941 taaattaata taaatgttat ttttttataa tttaatctaa aaaaattata ttaaaaagtt
    33001 taaatatagt ataaaatggg taagaaagta gtgaaaatta aatggcaaaa ttagggaaat
    33061 ctaatgacgc aaaactatag ggattaaagc ttttaaagcc atatcagcca gttgctaaaa
    33121 agagtaaagt ctttttattt tataattaag attctctttg agtgtctttt tttattgtct
    33181 gaaacttata gtatttaaat tcatgaaaaa ttagatattt attaagggag gattgttatt
    33241 tcatgaaaaa ggcaatatct tgtgtactag cagtatctat gtgtagtgca ccactaaacg
    33301 tttttgcaga acctatatta gaggggaagc taagggcagt tgaaaaatca gttacagaaa
    33361 agttaagggg gagtcttgaa gtagatttaa atttttcact gccaataaaa aatagcgagt
    33421 cagaaagtat gactaatatg cagttacgat taaaagatga tagtaacaat agtggaataa
    33481 taaagcttgg agaaaaagat tcaggaacag taaatgttgg gacagataat atagaatata
    33541 ctataaaaaa attatctgca agtagaagtg aaataaaaaa taaagatgag aatatatcat
    33601 attataatat tgtatttaat aatttacctg ttggaaaata caatgtagaa gtctctggag
    33661 atggatttaa gaacaaagaa attaaagaca ttgatatatc aagttattca caaagagtct
    33721 tattaagcaa tagagcaagt gtagataata aaaataaacc tttaaattat gaattgttct
    33781 taatgggtga tgtaaatgga gacggtaaag tagataagag tgattataat aaggttttag
    33841 agaatataga ttcaaataaa agagaatttg atttaaatag agatggaaag gtagatattg
    33901 ttgacttaga ttatgtacag aaaaatttag gacaaagtga agatgcaaaa agcctagaaa
    33961 gtatagtatc taccaatcct atagtagata caagtaaggt tgaattaaaa ggaaatagtg
    34021 atgtagatat taatggtaat gtagaaaatt tatttagtgg agaaaataat ggagttacaa
    34081 tatcttcaaa aaatgatgaa atatctgagg aaaaaccagc agtaatggat atagctttat
    34141 cagaagcact taatatggaa gtcatcaata taaaagctcc agaaggtact gctccagcaa
    34201 aaggaatagt tgaggttact gacaaagatg gtaatgttca acagattcct tatgaaaaca
    34261 aagaatatat gaaaaactta aaagatgagg agcaacaaga ggaagtaaat aacgaacaag
    34321 aagaagtaaa tcaagaagaa gtaaataata aagatgaaaa tataaaccag actgataata
    34381 actcaaataa taaagatgaa aataatgata aacaacagga aaatagcgat aaacaagaag
    34441 caaactctaa taccaatcaa gataaaaata atgataaaga aaattcatca atggataaac
    34501 ctaattcaga tgaagatact ccaaaagaag agggtaatat cagtaaagat gataactcta
    34561 caacatctac atatagtagg tcagcattag aaacatatgc agatgttcaa aatagtgata
    34621 tagtaataaa tcttggtaaa caagtagcag taaaaaagat aactataaaa ataacagcta
    34681 caacagcaaa cgatagaaat cttgcagaaa tatcaaaagt tgaattttta aacaatgtat
    34741 ataaagaaat accaaagcca gagatgaaca tacctaaaat aaataaaata tcgactagca
    34801 ctgctgttgg aaatgaaaaa gtatcttttt attggaataa tgaagtaaat gtatcagcat
    34861 acgaacttat actagacaaa attgatagta agggaaaagt attatctact aaaaaattac
    34921 aaactagtaa aaatacaata gaagttagag atatagaagc atatggacta tatagagtta
    34981 gcatacaatc tttaaatgga gattggtcta gtggttataa agatgcaaca ccagaaaact
    35041 tagacggagt accagaaaat gtagattcaa actataaacc tatacaattt agcccagata
    35101 gtatattaga atttcaagtt gttccagaca caaaaccaga accaccagaa ggtataaatg
    35161 ttaaaggtaa atttaaatct cttgatgtaa gctggaaaaa tcataaacaa gctaaagatt
    35221 ttgacttgta ctataaagaa aaaggctcta atgggtcttg gataaaatac aatgataagc
    35281 ttattcgtgg gacaagttca aagatagaag gtcttaagga taatacaaca tatgaagtca
    35341 gaatgacagc tactaaccac ttgggaacta gtggaatgtc taaaacatat ataggtacaa
    35401 ctgaaagcat ggatgcacca acaacaccta actatagatt aattaatact cctaaagaaa
    35461 atggtggatt tggaatacca acaaatcaca ttgtagatgt tgtttatcca gatggatatg
    35521 acaaaagtga atatagtgat aaaaatccat ttgataaatt caatgtagta gatggggact
    35581 ttacaacaca ttggacattc ccaacatata atgcagctga gggaacgaat agaggaccaa
    35641 taataacttt tgataaatct tatactatgg atacttttat ggctacacct cgttttgatg
    35701 tttctgataa gacaataaat gactttgagg tacgatattg ggatgaaaat ggtcaattac
    35761 acaatctggg ggatgttcct tttgaaacaa agacagaccc tgtaaataat aataagtatg
    35821 taatggttag attaaaagaa ccaataactg ctaccaagat acaagtaagt gtttgttcat
    35881 cctattcata taagtctaat atatcagaac ttaagtttta tgagtatgac agtatagaag
    35941 atgatgtaag aaatctattt atggatggcc tacaagttga attaaaagaa gatgtaactc
    36001 aagaaaaaat aactgaatta aaagatagat taaatactcc agataaagct agtggagaat
    36061 atcatccata taagacagtt attgagagag aattaaaatt agctcaagac ttatataatg
    36121 atagagccac acttactgat gtaactactg taaatcaaga aataaataaa ttaggaaaat
    36181 ataaagataa aaacggacag tatactgtaa attccaataa tttaggtatg caaaatgact
    36241 ggcaggcatt aggagtagct gctagagcag gagatgaaat cacagtatat gttggaagta
    36301 aaagtggaaa aactcctaag ttgatatata cacagtacta tggagaatct ggtgcatata
    36361 agagtggaga aataaatctt aacactggaa aaaatgtaat tcaattaagc aaacttcata
    36421 gtttagatat agaatgtgga ggaagtttat acataagata cgctgaagat actccaagtg
    36481 gaggagatat aaaagtacgt gtagcaggag ctactaagat accacatctt aatctaaatg
    36541 gaacaataaa tgataagagt cctgcgggag tttctgaatc taagaaaaag ataaaagcat
    36601 atatagaaga attagctaaa tataaagatg ctgtaaaagg agaaccatat tatccaggaa
    36661 caaatgttgg taattcatat ggatattcag aaaaaactgg tgtgttaaac actacagata
    36721 ttgaaagtga taaagtaaca ttaaatgttc ctgcaacagc agtatatcaa gagatatcaa
    36781 aaggaaattc tgatttaaat acacaagtag atagattata taatagtatg cttgcatggg
    36841 aacaaataat ggatttagtt tactcagaaa gaggagtatt taaaacacaa gatttagatg
    36901 gtaatggaac tgtagatgat aatgaattta acttaactaa aaatgatatg gctccaaaat
    36961 ctcgtatgaa cataaaatac caaagaatgt ttattggagc atttatgtat gcttctggat
    37021 tacatgtagg agtagaatat ggttcagttc cagggcttat gaatggagta cctttccaaa
    37081 tagatgatag tggaaaagct acaggaggaa acttatttgg atggggaata ggtcatgaga
    37141 taggacatgt tacagatatt ggaaagatga catatagcga aactagtaac aatgtacttg
    37201 ctttacttgc tcagaccttt gatgataaga cacattcaag acttgaaggt tcaacaatgg
    37261 ataagatata tgagcatgta acttcaaata gcttaggtat accaagcaat gtatttgaaa
    37321 ggcttggaat gttatggcaa ttacatctag cttatgatga tgattttacg ggttcaatgt
    37381 taaagaataa ctcagatgct gatttaagta atgatacatt ctatgcaaag atttcaagaa
    37441 aatacagagc actttcaagt agtgacccaa ttaatagttt accaaaagac caaatgctag
    37501 tagcaatggc atcaagtgtt gttgaaaaag atttaagaga ttactttaag gcatggggag
    37561 tagaaattac accagaatta aacagtataa tggatagcaa gaactacgaa aaagaatcaa
    37621 gaaagataca atatgtaaat gatgaagcta gaagaaagat tttaaatgaa aatatagcta
    37681 gtatggcaaa agatactaaa gtaagtgcta gtttttctga tggaatacaa agtggttctc
    37741 tagttaaatc taataaaata tctatagacc tagatgtaga taaggataaa gataagatac
    37801 taggatacga aataataaga agtgatggaa attatttaga tggtgaaaat ggaacacagg
    37861 taaaatacag aacagttgga tttgtggatg caaaaggaaa tagcaagact aaatttactg
    37921 ataatatatc tcctcttaat aacagagcat ttacatataa ggtagttgca tatgattatc
    37981 acctaaatcc aacagaagaa tttgaagttg gtactgtaaa attatcagat gaagggaaaa
    38041 taaataaatc agcatggtca ttcataacaa atactgtttc tgacggtgat gttagaactg
    38101 aaaatgactc acatggacca ttacaaaatc ctgaaattga taatataaaa gataatgatg
    38161 caagtactac atataaagga aagataataa gtaaagcaga gtggaacaaa gaccctcaaa
    38221 aagaccctga tataaatgta gaagaaaatc catatataat agtagacatg aaagaaacat
    38281 tacctatagt aggactaaaa tatacaaaac cagagacaga agctagaaag ttctcattaa
    38341 aaggattatt taactttaga aagaatacta gcacaacata caatccttta actaattaca
    38401 agatacaagt aagtaatgat aaaaagaact gggaagatgt aagtcaagga acttttgaat
    38461 atggacaaga tttagttggt ggaggaaaag accaagataa tgaagctaga gttacattta
    38521 ataaagatgg taaactttgg acataccaag ctagatatgt aaaactaatc tcaaataata
    38581 agtctaatat agaagttgca gaattagata taataggacc tccaggtgat aatatagaaa
    38641 ttggcataat aaataatgca gacgaaactc gtactaatgg aattggaaaa ttagcagaag
    38701 attatgtata tcaaagtgat gactctagca caagtgaaga tgaaacaggt aagattccag
    38761 cgggttcaat agttattaca ggtgaataca gtggaaatcc agcatttaat ttaccactac
    38821 ttatagataa aaacaataag actatatctg gagaagcact tttatttgca gaagttccag
    38881 aaaaaggtga actaggtgaa atcagtaaag gtacatgggt ttactatatg ccaaaagaaa
    38941 actttgattc tttaagtgat aaggttaagg ctgaactata tagatacaat gaccttcaag
    39001 gagatactcc agtaggtcaa agatttgtaa gtgatacaat gtatgtacct gtaggagcta
    39061 agaattatga tgagttaaag acaataagtc ttactgatag taatagtaaa gctagaaaag
    39121 ctacaagaaa ttctattgat atcagtaata agaaggttat aactgtaagc aataaagtaa
    39181 agggtaatat tgttagagat aaatagtagg tggaactcta aaaacgtaaa aatactaaca
    39241 ttgtatatct aataagatta ggtactagaa tagttttaaa atagtagata ttaaaagtaa
    39301 agtatataaa tgtaaccata aatttagggt aatttataaa ttaaatataa aagctaggtg
    39361 gataagtaaa tttatccact tagttaatga tagataagga cgggtgttaa tgagtaaaag
    39421 aaaatctttt ataagaacta tagcagtatc aactatggca gtagctgtaa ctggtagtgc
    39481 tacatgtgca tatgcagctc cagttttaca aggaacaaaa acgtatgaga aagtaaatac
    39541 tatagatata tctgtggact cagtagaaaa catagtatat tcatttcaag ccagtataaa
    39601 agtacaaggt gaggttgaag tagtagataa tgagcagaaa gaaaagatta cttggtctga
    39661 taatataaaa tctcagataa aaagtggaaa tgcaaatgct acttgtagag tagaatacaa
    39721 caaatcaagt aatacaacta cattagatat atatgttaca tcaaatgaag atttactaga
    39781 cggaaataga ttgaatatag gaagaatatc tgtaaaaaaa tctggcagta actcaaatgc
    39841 tgactataaa gttttaggaa aaggaacaaa tgataaacca gctttaaaaa ttgtaacata
    39901 taacaacaaa acagtggatt atgaaaatat aagctctgat gaaggattaa tatttaccct
    39961 tattaatgag agtgaagtaa aaccaattgg tggaactggt tctagtaaaa atgaccctga
    40021 aaagtataag gtagagaaaa gtgaagcact tgaatattta ttaaacaata ttaggataaa
    40081 ctattctatt gttagtaaag aaacacaaga aagtggttca aatgtaatat taaaacttgg
    40141 attagcacaa aaaacaacta agggaagaaa agctacaata aacaaatatg ttgaagttac
    40201 acttccaaaa agtttagaat acatagttga aaatgaatta tcaaaaccgg atgaattacc
    40261 accagataat ggcaatggtg gaaataatgg tggaggaagt aattcaggcg gctcatctag
    40321 tggtggaagc tctggaggag gaagttcaag tgattctaca tctaatgtca cagtcaaaaa
    40381 attaaaaggt gctgatagat ttgagacagc tatcaaaata tctcaaagtg gttggacaaa
    40441 atcagataca gttgtaatag taaatggaga agataaaagt atggtggatg ggcttacagc
    40501 tacaccactt gctagtgtaa aaaactcacc aattctttta tcatcaaatg aaaagcttcc
    40561 tcaaaaaact gtagaagaat tgaaaaggct aaatccatca aaagtaattg tgataggtgg
    40621 aaacaactca atgccaaatt cagttgttga agctatcaaa gctgtaaatt caaaaatatc
    40681 agtacaaaga ataggtggag atacaagata tcaaacttct attaatatag caaaagaaat
    40741 agataagacg aataatgtaa gcaagttgta tataggagca ggaaatggag aagctgattc
    40801 attatcaata gcatcactgg ctggtaaaga aaaaactcca atagtactta cacaaaaaga
    40861 tggtgtagat aatgaagctg aacaatttat taaatcaaat aaggtgtcaa atatatattt
    40921 tattggtgga gtagaaaaaa tatctaacaa ggctattgaa caagttggaa aaatagcaaa
    40981 caaagatata tcaaacaata gggtggcagg tcaaactaga caagagacga atgccaaagt
    41041 aatagataaa ttctactctc aatctaagct agatggagtt gtagttgcaa atcaagataa
    41101 acttatagat gctttagcag ttggaccatt agcagctaaa aacaattcac cagttatact
    41161 tgctacaaat acgttagata aatcacaaga atcgtcatta aaaggtaaaa attcatcaaa
    41221 gttgtttgaa gtaggtggag gaatagcctc atctgtaata gacaagatta agagtttaat
    41281 tgaaaaataa atattaagta aatagaaaat aagaatgtat ttagatataa agttatatat
    41341 ttaaatgcat tcttatttta ttttcattat cttaacaatc taataatatt gaggttaaaa
    41401 tgtataataa ttt
//
LOCUS       DAMJVI010000021        40975 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.021, whole genome shotgun sequence.
ACCESSION   DAMJVI010000021 DAMJVI010000000
VERSION     DAMJVI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.021"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(1..2493)
                     /locus_tag="PC545_001247"
     CDS             complement(1..2493)
                     /locus_tag="PC545_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456024.1"
                     /translation="MNHNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     ICIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIVLILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGIAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCVSLIFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKSLLN
                     KVQSADTTNITNITKEISTTMFDEKVPSIITKIQKNVQKGIDGTQSGINGIEDGENKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGVDKGIKGMEQGLEKQDEAIKELTT
                     AYNKIPTTEKTPTDNENNSNEQNQSNTSNSESDDTKNDSTENKNNNSSENKPNVNEQK
                     ESLNAQIQQLKKTRDDLNTKLQKNKTQKNELSKKLRSMESQKKELQSKLNNSISQKKD
                     MEKSLDTMQQQKKSLQAVLAKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFIILLFYKENKTR"
     gene            complement(2507..3049)
                     /locus_tag="PC545_001248"
     CDS             complement(2507..3049)
                     /locus_tag="PC545_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456025.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLNIIFTEIRNFINKVHNIQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3293..4807
                     /gene="glpK"
                     /locus_tag="PC545_001249"
     CDS             3293..4807
                     /gene="glpK"
                     /locus_tag="PC545_001249"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDF2456026.1"
                     /translation="MEKKYVMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDEDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASVQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVDIDRPKIVETTALGAAYLAGLS
                     VGFYIGRNEITSKWSVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4921..5550)
                     /locus_tag="PC545_001250"
     CDS             complement(4921..5550)
                     /locus_tag="PC545_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456027.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRSVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAQTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6138..6929
                     /locus_tag="PC545_001251"
     CDS             6138..6929
                     /locus_tag="PC545_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HDF2456028.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVDKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNVEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            6958..8178
                     /locus_tag="PC545_001252"
     CDS             6958..8178
                     /locus_tag="PC545_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDF2456029.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8198..8893
                     /gene="pxpB"
                     /locus_tag="PC545_001253"
     CDS             8198..8893
                     /gene="pxpB"
                     /locus_tag="PC545_001253"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDF2456030.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLEIDFDSLIENLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEVPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8923..9909
                     /locus_tag="PC545_001254"
     CDS             8923..9909
                     /locus_tag="PC545_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HDF2456031.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSIAGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYINTFIQEKDF
                     TINKKLSSKYIPTYSSEIVLRIVKGPQFDAFSNGEVEKFLSNKYKVTNEIDRMGCRLD
                     GESIKHLNGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10104..10670)
                     /locus_tag="PC545_001255"
     CDS             complement(10104..10670)
                     /locus_tag="PC545_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456032.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTTEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDICMGEKVVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11022..12089
                     /locus_tag="PC545_001256"
     CDS             11022..12089
                     /locus_tag="PC545_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HDF2456033.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVLSVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNAPLYKVLGGFDSKVQTDITISIDKPEKMAVEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNTKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEELEGISGGF
                     VMDGDIMNLVNKPGLGIEVNL"
     gene            12108..13268
                     /locus_tag="PC545_001257"
     CDS             12108..13268
                     /locus_tag="PC545_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2456034.1"
                     /translation="MNTLFSNPGSILAVMTSMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPISMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFASFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13281..15377
                     /locus_tag="PC545_001258"
     CDS             13281..15377
                     /locus_tag="PC545_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HDF2456035.1"
                     /translation="MIKFKLYISGLYSGNVIFDGDLLIEKLNPFTNKIESLKPISKEE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWEKTHKNEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPDFVDSAKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWEPMIHEREDGDSTINWIVSQEWSSGIVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNSMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPVEYYSVGSNKWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDNDLEEKYDSYIYNPKDPSVQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDAFSEEF
                     TVTGDIKLEFFASSSAKDTDWVIKIMDVDLDGNSIKLADGILSARFRNSFYKSEFMEE
                     GEIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15536..15880)
                     /locus_tag="PC545_001259"
     CDS             complement(15536..15880)
                     /locus_tag="PC545_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2456036.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEVLAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            16172..17062
                     /locus_tag="PC545_001260"
     CDS             16172..17062
                     /locus_tag="PC545_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2456037.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            17280..18566
                     /locus_tag="PC545_001261"
     CDS             17280..18566
                     /locus_tag="PC545_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDF2456038.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKHDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHIDDLCDKYKDKIFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTEENLLNILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKEFGKNEEIVMNPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDSAKQMTKEIEETIF"
     gene            complement(18655..19098)
                     /locus_tag="PC545_001262"
     CDS             complement(18655..19098)
                     /locus_tag="PC545_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HDF2456039.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFGITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDRDKKKP"
     gene            complement(19122..19916)
                     /locus_tag="PC545_001263"
     CDS             complement(19122..19916)
                     /locus_tag="PC545_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HDF2456040.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEITAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKKIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
     gene            complement(20482..20958)
                     /locus_tag="PC545_001264"
     CDS             complement(20482..20958)
                     /locus_tag="PC545_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2456041.1"
                     /translation="MKEILINPIGKICIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTAVQIINIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSVDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
     gene            complement(21059..22228)
                     /locus_tag="PC545_001265"
     CDS             complement(21059..22228)
                     /locus_tag="PC545_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2456042.1"
                     /translation="MLNIKTLCHEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVSPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLEKPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTNTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIEGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIRE
                     ICDFTCKTFGGSISVEIEEGYPALINSNHLVDYVKQNAVELFGEENIILKDSPTLGAE
                     DFSYFLRHCEGAFYHLGCANREKNITSPLHTSTFDIDEDCLITGVMLHVKNVLSF"
     gene            22391..23254
                     /locus_tag="PC545_001266"
     CDS             22391..23254
                     /locus_tag="PC545_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDF2456043.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLNYKGYL
                     AGNDDIRARDIEDMFLDKDVDIIMCLRGGYGTTRILDKINYDIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVGKWGKFTYKSLVNALEFKDELYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEENDLMELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            23325..23918
                     /locus_tag="PC545_001267"
     CDS             23325..23918
                     /locus_tag="PC545_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HDF2456044.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(24169..24804)
                     /locus_tag="PC545_001268"
     CDS             complement(24169..24804)
                     /locus_tag="PC545_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2456045.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAR
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTPKSIELLSYPVHCLENDVES
                     ELVDELKSIENLQILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(24884..25615)
                     /locus_tag="PC545_001269"
     CDS             complement(24884..25615)
                     /locus_tag="PC545_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2456046.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLDMDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLRKLKTLNSSFEGYQSNKECKIPTLEEVLILISPTD
                     LILNIELKTDKINYPNIEKDVLELILKYNMKNRVLISSFNSNSLKNFHKLDPSIKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIELCHKNLLGVNVYTVNEEGDILHCL
                     KLNVDGIFTNYPDIASNLLHSKQYS"
     gene            25946..28135
                     /locus_tag="PC545_001270"
     CDS             25946..28135
                     /locus_tag="PC545_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HDF2456047.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDNDILIEDL
                     EFVCKEEEKVLLEDFNNNQYEIPYDKSYVDLFREQVKVTPSNIAARDENRKLTYKELD
                     EVTDKLAGYLNHIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLGYELTEKITSSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSISATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYRKRNYELYNEYGPTEFTVVSTSFHVDREYGKIPIGKPIFNTYAYVLDKKNKLCPI
                     GAPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDDGDMYICAYYVIDEED
                     SGKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKRALPE"
     gene            28546..30474
                     /locus_tag="PC545_001271"
     CDS             28546..30474
                     /locus_tag="PC545_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HDF2456048.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFFEGLAVGVGGVLFGIGIAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSAGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVSG
                     MTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGSK
                     NEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGSL
                     PWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEKE
                     KEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVLV
                     VLTALPIVLSKVKNNEQE"
     gene            30461..30808
                     /locus_tag="PC545_001272"
     CDS             30461..30808
                     /locus_tag="PC545_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HDF2456049.1"
                     /translation="MSKSNDVLDIVFKITDGLEYEVAENRVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(31050..32969)
                     /locus_tag="PC545_001273"
     CDS             complement(31050..32969)
                     /locus_tag="PC545_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_071223118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2456050.1"
                     /translation="MNLMTLENISKSYSEKKLLENISLGINEGEKIGLIGVNGTGKST
                     LLKIIAGVEESETGNITKTNGIRIEYLPQNPTYNENSTVLEQVFNGTSEELKLLGEYQ
                     EILEKLNSNFTEELNKKLLSVHEKIDALNLWDLESEAKSVLTKLGINDFNQKIAELSG
                     GQRKRVSLASALITPCEFLILDEPTNHLDNDTIDWLEEYLNSRKGSLLMITHDRYFLD
                     RVTNRIIELDKGRLFSYDGNYSIFLEKKMERLSLEASIEDKRQNLIRTELAWVRRGAQ
                     ARSTKQKARLQRFDELINRDSFKPDENLDISVASSRLGNKIIEIHNISKSFEENTVID
                     NLEYTLTRTDRIGIIGKNGMGKSTLINILNGEIEPDSGYISIGETVKIGCFSQDDSHM
                     NIDMRAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKLSGGERRRLHL
                     LKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYFLDRICNKIFA
                     YEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSTTLKKEKPKNDKKLKFS
                     YNEQREFENIDSDIEKLESKIATLDESTAKFSTDFTKLQEILDEKSKLEKELEYKYER
                     WEYLNNLADEIANNK"
     gene            complement(32995..33801)
                     /locus_tag="PC545_001274"
     CDS             complement(32995..33801)
                     /locus_tag="PC545_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2456051.1"
                     /translation="MNLILGDNMSKKSKRRKINKLKLTIVITVLFLALLSIYEILFSN
                     PNKKGNIKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     IKLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVVSNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIKKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            complement(33860..35308)
                     /gene="ddlR"
                     /locus_tag="PC545_001275"
     CDS             complement(33860..35308)
                     /gene="ddlR"
                     /locus_tag="PC545_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HDF2456052.1"
                     /translation="MIFSNLKLNDNEPIYIQLKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSEGLIYSISGKGTFVKSKNKSSNTTWSLNWDCLENTYSKK
                     ANELDIIKSEIPWSSDLISFKSISPDGDLFDIEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLEYMTNKGADISNKDILITNGFTEGFDIIMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFNMLEDNLVKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYKCIKKYIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            complement(35332..36231)
                     /locus_tag="PC545_001276"
     CDS             complement(35332..36231)
                     /locus_tag="PC545_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDF2456053.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVEEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            36489..37745
                     /locus_tag="PC545_001277"
     CDS             36489..37745
                     /locus_tag="PC545_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDF2456054.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFDESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILKKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEREAFKLIKPVEEKIKSRVGKYVYTEGDISISEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVTYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVKKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            37803..38582
                     /locus_tag="PC545_001278"
     CDS             37803..38582
                     /locus_tag="PC545_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2456055.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDIVAIEEIEKKGVQFNVATGRI
                     FKQAHNIIKDSLDMNGYYVCENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(38686..39270)
                     /locus_tag="PC545_001279"
     CDS             complement(38686..39270)
                     /locus_tag="PC545_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HDF2456056.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(39470..40900)
                     /locus_tag="PC545_001280"
     CDS             complement(39470..40900)
                     /locus_tag="PC545_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2456057.1"
                     /translation="MNLDLEFYQKILEASHDEICVSDDKGIIIYCNKAFEENYGLKKE
                     DILGKNVSFLEDSGYSTKSPIPIVLKTKTKFSLEQDTQTGKKLIITATPIFDENGNLE
                     FTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVMRPII
                     NTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRSNGPFITINCATISPQLLESEL
                     FGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFTPVGS
                     LKNKKVDIRIISATNVDLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVEIIKY
                     YFNRYSSDFNLNKTISKEAMDAIANYRFPGNIRELQNIIQKILLTCTDNHITIDDLPN
                     ILTKNIHITNNGNKTHISQINKVIISDSKSTNYKNKNFDTLIKEYEKNIILDAYEKFG
                     SSYKVAKHLEISQSKANRLIRKYTNT"
BASE COUNT        14902 a         5085 c         6488 g        14500 t
ORIGIN      
        1 ttatctagtt ttattttctt tatagaacaa taatattata aatgctagta gatttacaca
       61 gaatactgtt atatacatct gtttaaatcc tgagtttaga gtagattgta gtgtgttttc
      121 tatttttgtt ctattatttt ctatggaatt tatataatcc aattttgatt tatcaaaagc
      181 ctttggtatt tcagttttta cctcttgtgt ctttgctaaa actgcttgga gtgatttttt
      241 ttgctgttgc attgtatcaa ggcttttttc catgtctttc ttttggctta tagaattatt
      301 taacttgctt tgtagctctt tcttttgaga ttccattgat cttagttttt tacttaattc
      361 gttcttttga gttttatttt tttgtaactt tgtatttaag tcatctcttg tcttttttag
      421 ttgctgtatt tgagcattta aactttcttt ttgttcattt acatttggtt tattctcgct
      481 ggaattgtta ttcttatttt ctgtagaatc attcttagtg tcatctgatt cagaattact
      541 ggtattactc tggttttgtt cgtttgagtt attttcgtta tcagtaggag ttttttctgt
      601 agtaggtatt ttattataag cagttgtaag ttccttgatg gcctcatctt gtttttcaag
      661 accttgttcc atacctttta tacctttgtc aacaccagca ataccttttt ttacaccatc
      721 tattccttga ttaataccag ctctagcttt taccatattg tcaataccag tttgaactcc
      781 tttaatacca ctatttagtt tattttcacc atcttcaata ccgtttattc cactttgtgt
      841 tccatctatt cctttttgta catttttctg tattttagta attatagatg gaactttttc
      901 atcaaacatt gttgttgata tttcttttgt tatattggta atgttggttg tatctgctga
      961 ctgaacttta ttaagtaagg attttggaag tttgccacct gtcatgtcca tatttatatt
     1021 tgaagtatca ttcatatttg ggatagagac attttttaaa ttttttgcca tttcagggtc
     1081 tgattttaag tcatctatca tgttttcaag ttcttcaagt tgaactattt taggagttga
     1141 gggctttcca actaagtcca taatattatc ttgcacagat aatcctgcat gtgctataaa
     1201 tccaatcatt atagctggag aaattgaagt accaattgag cgtatcaagg ataatgttgc
     1261 aagtgctgaa ttagattctt ctaatcttgt atttgataac atcatatagt ttagaggagt
     1321 tcccatagta aatcccattc ctaatcccat aaatattaaa cttacacata cactaaatac
     1381 agtattggtt tttagagcaa ttaaaactag gtaaagagag cctatcatag atacagaaaa
     1441 tcctagtaat agtatttttt ttgccccaaa cttgtcaata agtcttccgc ttaaaggagc
     1501 agcaatacct gcaaataatc ccaatatagc tacaaaatat ccaccagaac cagcatttat
     1561 ctttaatgca ttttcagcat actgtggaac gaataccata cccatcattc caataccaac
     1621 aattaaagat aaaataagta ctatcaatat tctcggattt aaaaaatatt caaagcttaa
     1681 aatagggtct ttagctctat tttcaataaa tatgaaaata ggtataagta caataaatgt
     1741 caataaatat ggataaactg atatatcttg tatagaattt ttaaaattaa aaaagtctat
     1801 attcataaga ccatataata gtgatacgat tatacatatc aacatcaatg taccaagctt
     1861 atctattttt tctttagatt cagacttatt gtttttaata cagaaaactc caccaataag
     1921 tatgattaaa caaattggaa gatttacaaa aaatagccac ttccaatttt gagttccaaa
     1981 tatacttaga attgtgctac caatgcttga acctaaaata ttggcaatac catatgttgc
     2041 cccaactaaa cctaaagcca tgcctctttt gttttctggg aaggtagttc caaattctgc
     2101 tgtagcaatt ggcatgattc caccaccacc aattgcttga ataacacgac caattaataa
     2161 aatatagaaa ttggaaaata gtgatgataa tccacatatt aatgaaccac taccaaatag
     2221 aaatatacta actagataca catattttct tcctatttta tctgctaatt ttccagagat
     2281 aggaattaca gaagcgtatg ttaatgtgta tatggtaatc atccaaattc ccgttttttc
     2341 atttactcca aggctatttt gtataatcgt tcttgctggt gatactattc cagtatcaat
     2401 agctcccata aatataccta atagataaag tgtaagtact aaaaatattt ttggattact
     2461 gtccttgttc ttaatactag tattatgatt caaggtaaac accaccttaa tgtattaatt
     2521 tttcaatata gggttttaaa tcttcaaatt ctatatgaag atatgttgaa tatgaaaaaa
     2581 acatttttat tatgatattg catactaatt tgtcattatt aaaaaaactt tctttttcta
     2641 aagcttcata tactttattt gaaagctgtt catgaaagtc gtctttgtgt tttttcattt
     2701 caaaaaatct ctctgtttca aaagtgttta aaaagtcatt gtattggata ttatgaactt
     2761 tgtttataaa atttcttatt tcagtaaaaa taatgtttag tttttttatg taatcttcag
     2821 tgtttttatt ggagtattca attcgcctta tcaataaatc ccattcagat tttgtgatat
     2881 attctattat ttcttgtttt gaattaaagt aattatacac tgtacctgca cttataccac
     2941 acctttgagc tattttcctt atgctaatat tcgagtaatc ttcttctaaa atcatatccc
     3001 gagacacttt taatatatct tcttttacat tttctaaaat ctttggcata ttaccacctc
     3061 ttttattaaa attattatga acactgttta ttatactcct ttaaaattta ttaatcaata
     3121 tgtaagctat aatgtataat attattatat agatatgata gaaaataagt gtcttcttac
     3181 taaatatcta ttatattaaa tttgtactta agtatgatta tttattgaaa tatgtgatat
     3241 taaatttttc tttttaataa attaaatatt atatgataag gagaatatgg tgatggaaaa
     3301 gaagtatgta atggcattag accaaggaac aaccagttca agagctatac tatttaataa
     3361 aaaaggcgaa atagtgaaaa ttgcacaaaa agagttcaat caaatttatc caaaagctgg
     3421 atgggtagag catgacccta tggaaatatg ggggtcacaa agtggggtaa tgagagaagt
     3481 tatagaaaca gcaggtataa gaccagaaga aattgcttca atagggataa ctaatcaaag
     3541 agagactacg gttgtttgga gtaggtatac tggtaaacct atttataacg caatagtatg
     3601 gcagtgtagg agaacgtcag aaatatgtga tgaacttaaa aataaaggat tggaagaatc
     3661 aataaaagaa aaaacaggtc ttttaataga tgcatacttt tcagcaacaa aagtaaaatg
     3721 gatattagat aatgtagaag gagctaggga aaaagcagaa caaggagagt tattatttgg
     3781 aactatagat acatggttaa tatggaattt aactcgtgga aaagtacatg taacagatta
     3841 ctcaaatgca tctagaacaa tgatgtacaa tataaatgat ttagaatggg atgaggatat
     3901 attacgagaa ttggacatac caataagcat gttgccatta gtaaaacctt ctagctatgt
     3961 ttatggtcat acagatgagc gcatgttatc aggagctaaa atacctattg cgggatgtgc
     4021 tggagaccaa caagcagcat tatttggtca aaattgtgtt gaagaaggta ctgcaaaaaa
     4081 tacttatgga acgggttgtt tcttacttat gaatacaggc agtaacatag ttaaatctaa
     4141 gcatgggtta cttacaacaa ttgcttgggg agttgatgga aaagtaacat atgcattaga
     4201 aggaagtata tttattggag gagcatctgt tcaatggctt agagatgaat taaagataat
     4261 tgaatctgca aaagatagtg aaatgtatgc aaatagggta gaggatacta atggtgtata
     4321 tgtagtgcca gcatttacag gtcttggagc tccatattgg gatatgtatg ctaggggttc
     4381 aattttagga cttacaagag gagcaaaaaa agaacatata gtaagagcca cattggaatc
     4441 aattgcttat cagacaaagg atgttttaga agcaatgcaa aacgattcaa aattaaaact
     4501 aaaatcatta aaagttgatg gtggagcaag taataataat tttttaatgc aatttcaatc
     4561 agatatatta aatgtagata tagatagacc caaaatagta gaaactacag ctttaggagc
     4621 tgcttattta gcagggttgt ctgttggatt ctatattgga agaaatgaaa ttacttcaaa
     4681 gtggtcagta gaaaaagagt ttaatccaaa tatgagtgaa gagaagagat gtaaattata
     4741 taaaggatgg aaaaaagctg tatcgagagc tttaagctgg gaaaaagaag atgaattgga
     4801 tatttaaaaa ttaatcaaag ttaaatttat ttgaatttaa aaagagcttt atgtaaaata
     4861 tgaagctctt tttttgttgt taattttgca tttttatata atcatactaa attatataat
     4921 ttatttttcg ttttcatata aaaacattgg tatagaattg taaatgtgtt ggtcaccaac
     4981 tactaagaga atgtctcctt ctaaaaattc aatatatggt ccaggagata ttataagttc
     5041 ttcacctctt ttaattgcaa ctattgtagc acctgtattt tgccaaaatt tagtttgtgc
     5101 agctgtttga ccaatatatt tgcaattaga atttatggtg aactgaacgg gcactaaagg
     5161 attgattatt tcaagtctgt tagaatagtc taaaatttta tttatagtat ctaatatttc
     5221 attttctaaa ttagatttag tatctattaa attataaagt gttgatttat atgatgatat
     5281 atttgtaata tctctatatt tttttataaa agaagaagct ttgtctggag atagtacaat
     5341 aacaccttta ccctttatgc ttttaacaac gccaatgtcc tctaaaattt tgacagatct
     5401 tcttatcgtt tctggagata cattgtattt accagccaaa acagagcgtc caaatagtac
     5461 tgaatcctct tgtatttcac ccgtgtatat tttatttgca atatccaacg ctattttttg
     5521 gtatattggc atcgtatagt ttttatccat tgtttttccc ccaatacaaa atttatgtta
     5581 aaaaaatata taatcttaac aatactattg tataatttat aggtttatat gtctacaata
     5641 tgtacaaata ttgtgcatgt tttaaaataa tattttaaca tgcatttagt atacatgatt
     5701 ttattatact taaaactata taaagaatta attataaaaa tataaagata ttttacttac
     5761 aaagaatgat aattatatag atgaaagaaa caaatgaatg aagaaaaatg attctgtctt
     5821 gcatataaag tgattaaaaa tgaaaaatac tttaaaaata aaggttaaag cccttatttt
     5881 aagtgaaaat aattaattat taactaaaaa atggtaaaaa gattttttga aaaagaaata
     5941 caaatgaatg ttcaattaaa ataattgcaa aaatattcct taaatggtaa aattatatta
     6001 attaaatgca atagaatagt tgcaaaaata aaattgaata caagtgataa aatatttcat
     6061 gcaatatata aaataaacaa aatttaaaaa ttaaaactac ttattctatc aaaatgttag
     6121 caaggagggg tcataaattg tataaagtag atttaaatag tgatttagga gaaagttttg
     6181 gaacgtataa gattggttta gatgaagaag ttttaaaata tatttcatca gctaatatag
     6241 cttgtgggtt tcatgcaggt gaccctagtc atatggaaaa aacagtacag ctagctaaaa
     6301 aaaatggagt gaaaataggc gcacatccag gttttctaga tttaatagga tttggaagac
     6361 gtgagatgaa aataacaaag caggaagcta aagattatac aaaatatcaa ttgggtgctt
     6421 taatggcatt tgcaagttca aatggatgta atatacaaca tgtaaaacca catggagcat
     6481 tatacaatat ggcagctaaa gataaagagt tagctatggg gatttgtgaa gctatatatg
     6541 aagtcgataa agatattata ctgttggggc tatataatag tgaaatgata aattctgcta
     6601 aagaaattgg acttaggttt gcaaatgaag tatttgcaga tagagcttat gataataatg
     6661 gttttttggt tccaagaaat gtagaaggag cagttataca tgatacaaaa catgcaatag
     6721 atagggttgt taggatggta aaagaaggaa ctgtagaaac tttaacaggt gaagttatac
     6781 atatcaaagc agattctata tgtgtacatg gggacaatcc aaaagcaata gagtttgtca
     6841 aagaaataag aaagcgtttt gaattagaaa gtatagaagt ttgtccatta gaaaacatag
     6901 aagtttgttc gtcagagaac atagtataag actgataaca tgagggggag ataaaatatg
     6961 agtacaaaaa atattacaga taaaactaaa aataaaaaag atgtcggagc acttataggg
     7021 gctgcattta taatggcaac atcagcaatt ggaccagggt ttttaactca gactgcacaa
     7081 tttacccaag attttggacc gaatttttca tttgtaattt taattacaac aatccttttt
     7141 ataggggctc aagtaaatgt atggagagta attggtgtat caggacttag aggtcaggat
     7201 atagcaaata aaataatacc aggattaggt tatttagtag cttttttagt agctcttgga
     7261 ggattagctt ttaatatagg aaatgttggt ggagctgcac ttggtatgaa tgtaatgttt
     7321 aatatgaata tgacattagg aactgtatta agtggattaa tagccatatt tgtatttatg
     7381 tcaaaaaatt caaattctct agtcgataag ataactaaat ttttagcatt aggaatgata
     7441 attatagttg gatatgtagc aatatcaaat catcctcctg ttggagaggc agtatcaaga
     7501 atggtaaaac cagaaaatcc aaaaggactg atattcccaa taataactct acttggagga
     7561 agtgttggag gttatataac ttttgctgga ggacatagat taatagacgg tggaataaca
     7621 ggagaagaaa atataaaaga aataactaaa tcttctcttt tgggtatatt agttgccaca
     7681 atgatgagag ttcttttatt tttagcaata ctggcagtgg tttcaaaagg tcttcaactt
     7741 gacccagaaa atccagctgc ttcagctttt aaatttagtg caggagcaat aggatataaa
     7801 ttctttggac ttgtattatg gtctgctgca attacatcag ttataggagc tgcttataca
     7861 tcagtttctt tcttaaagac actaaatcct tttatagata aatatgaaaa atattttata
     7921 atagcattta tagcaatatc aacacttata atggcattta taggaaaacc agctacttta
     7981 ttaattttag caggagcttt aaatggattg atacttccaa tcacattagg aattatgctt
     8041 atagcatcta aaagaaaaga tattgttgga gattacaagc atcctacatg gcttttaata
     8101 tttggattaa tagtagtttt aatatctgca tatacaggaa taacttctct tagcagccta
     8161 ggtgctttat ttgcttaatt tagaagaggt gacatttatg gaaactagat acttgatatc
     8221 aggggataag gcagtggttg ctgaatttgg aaatgagata tcagaagata taaacaaaaa
     8281 agtaatttca tttatgagag caatagaaat atcgaatctt aaaggtattg taacagaaat
     8341 ggtaccaaca tataggtcac ttatgataag ttataatcca ttagaaatag attttgatag
     8401 tttgattgag aatttaaaaa aaatagaaga taatttggaa agtatagtat taccaaaacc
     8461 taaaatacat gagattccag tatgttatga tgaggttttt ggtatagata ttaaaaacgt
     8521 tgcaagctac aataatctca ctgtagatga agttataaaa atacacacaa gcagagagta
     8581 cttgatatat atgctaggtt ttactcctgg atttccatat ctaggtggta tggatgaaag
     8641 aatagctaca cctagattag aagtgccaag gactaaaatt tatggtggga gtgttggaat
     8701 agcaggttcc cagactggtg tatatcctat tgatagtcct ggtggatggc aaataattgg
     8761 taggacacct ttaaaattat atgatgaaaa tagagaagaa cagattttgc ttagagctgg
     8821 agattttata aaatttgttc caataacatt agatgaattt atagaaatcg aaaaaaataa
     8881 cttaagtatt taaatatcgt tatagtacag ggagtggtat atatgggatt caaagttgaa
     8941 ttaggaggtt tccaaacatt aatacaagac agaggtagag ttggctatgg tcaatatggt
     9001 gtatctggat gtggagcaat ggatgagtat gcacatagag taggaaatat attagtagga
     9061 aattctgaag atgaggcttc tttagaagtt ttaatgttag gtcctacaat tacctttgat
     9121 gaatatacgc aaatagcagt aactggggga gatttagggg ctaaaataaa tggaaaagaa
     9181 attcaaaact ggaggtcata tcaaataaat ccaggtgatg tattatcatt tagaggtgtg
     9241 aaatcaggtg ctagagccta tgtgtcaata gctggtggaa tagatgttcc acttgcaatg
     9301 ggaagtaagt caacttatac aagagctaaa attggtggat ttgaaggtag agcacttaaa
     9361 aaaggagatt atataaatac ttttattcag gaaaaagatt ttacaataaa taaaaaatta
     9421 agttcaaaat atattccaac ttatagttca gaaatcgtac taagaattgt aaaaggtcct
     9481 caatttgatg ctttttctaa cggtgaagta gagaaatttt tatcaaataa atacaaagtt
     9541 acgaatgaaa tagatagaat gggttgtaga ctagatggag agagtataaa gcaccttaat
     9601 ggagcagata ttatatctga tggtatttct tatggagcta tacaagtacc tggtcatgga
     9661 aaaccgatta taatgttatc agatagacag actagtggtg gctatactaa gatagggaac
     9721 gttataagtg tagatttgta taagttagca caagcaaagc caaatgatgt agtaaaattt
     9781 gaacttgtag atatatatga agcacataga ctattgagag agcaagaaga taagattcaa
     9841 gatatctaca agagtatgaa aaatataaga gttgtaaagg caaaagtatt aaatgatata
     9901 gcagtttaat tttaacaaat acaattttta ttataattta taatataact tacaaatagc
     9961 tatttactat ttaccataat taagatggtt tagattagtc aaatagctat ttttttagtt
    10021 tgagtaaata ttttaaattt aaaataaaag caatatttaa atctattata ttatctacaa
    10081 aaagtaggtg tgtcatattg agtttattta gattttattg gccaaattgg aggtgatata
    10141 actccatatc cacgagctat tgtatcttta cttggattga tgattttatg tggtacatta
    10201 gcttttataa atgttgagtc acctggattt aatctaacta ccttttcacc catacaaata
    10261 tcaaccacac cactcgttac caccataatt tcttctgatg catgatatat ataatctttg
    10321 gcatcctcac tttctggttt aagttcaaac tctacaaata aaattgaagg tgtaaactca
    10381 gttgtaggca ttggagatac tatctcgtaa aacatttcac tctttggaaa tttcatcata
    10441 actctatctt cttttctaat gactaaatca tcagtattat tgtcatccat aaacatatat
    10501 aaaggagtgt ctaaaacttc agcgattttt ctcaatgaag acaaggatgg ttcaatagca
    10561 tctctttcta tttgagagat ataacttata gataaatctg tcaatttgga aacatcactt
    10621 aatgtttttt tcttgctttt tcttatattt ctcatctttt cacctagcat aagaacctcc
    10681 aaataataaa attaatataa aatattcgct taaaacaaag taattatagc acaacataga
    10741 agtagtggca ataagaatac atagtagatt ttgacaatta aaatttaatt aaatccaaat
    10801 aaaagtctat gaaaatgaat tattgttaaa ttaatacaaa gaaaataata ataaaaaaat
    10861 ttaatagaag agtattaatt gaattaagat tatcacatta tattttggaa aagagtaaat
    10921 tttcagtaaa ttatcttgac atatatattg aagtaataat ataatttaaa gtataaaatt
    10981 ttaaaaattg aattataact tcaaaaaata aggagggtaa tatgaagata acagatatca
    11041 aatttgaaaa gcttagaatt aaattaaaaa aacctgtagt agtttcattt ggagtaattg
    11101 agtatggaga aagtataatt ttaaaaattg aaactgatga agggtattgt ggatttggcg
    11161 aagcagcacc tcttgcagca gttacaggtg aagttttgga taatgttctt tcagtattgc
    11221 ttatgtttaa aaaagagtta ataggcaaag acccactaga tattgaaacc atacatacca
    11281 tcatggatgg aattattata ggtaatactt ctgcaaaagc agctattgac atagctttat
    11341 atgatattaa aggaaaaatt atgaatgcac ctctttataa agttcttgga ggatttgata
    11401 gtaaagtcca aacggatatt actataagta tagataagcc agaaaaaatg gcagtagaag
    11461 ctttagaaag agtaaaagaa ggttttagga tattgaagct taaagcaggt ataaatcctg
    11521 aagacgatat tgaagctgta aagttaatta gagaagccgt tggagagagt ataagaatta
    11581 gaattgatgc aaatcaagga tggaacgtaa atagcagtat aaatactatc aaaaagttag
    11641 aagaatttaa tgttgatgca attgaacaag cacttccaca ttgggattta gatggtactg
    11701 cttacataag aaataaaagt aacacaaaaa ttatgataga tgaatcatta cattcaccaa
    11761 tagatgcaat taaggctata aaaaagaatg cagttgatac atttaatatt aagctgatga
    11821 aatcaagtgg aatatatcct gctataaaaa tcaacaacat agctgaggca agtggagtaa
    11881 attgtatgtt aggatgtatg ctggaaacga gaattggtat aacagcagca gcaaatttaa
    11941 tagcatctaa aaagaacata acagaagctg accttgatag ttttatgttt tgtgaagaat
    12001 tagaaggtat atctggagga tttgtaatgg atggagatat tatgaatctt gtcaataaac
    12061 caggtttagg aatagaagta aacttatgat aaatttggag gttaaatatg aatacacttt
    12121 tttcaaatcc aggtagtata cttgctgtaa tgacatcaat gattgcattg ggtttttact
    12181 tacaaagata caaagccatt aaaagcttag gaccagcact tacaataatc ataatgggaa
    12241 taatactttc aaacttaaaa gtagttccag taagtactga gttatatgga actatttcaa
    12301 catatgctat tccaatttct atgactataa tgttgatgag tgttgactta aaagaaatga
    12361 caaaattatc aagagaacct ttaattgcaa tttttgtagc tgtattaact gtaagtatca
    12421 tggcattttt atttggatta gtatttgcag aaaaaatatc agaaggttgg aaggttgctg
    12481 gaatgtttgt aggtacatat actggtggaa gtgctaatct tacagctatt ggaacaggtc
    12541 ttaatgtaag tagacaaact cttgcagcag caaatgcagc agactatgta attggagtac
    12601 caacattgat atttatgttt gctctaccag caatattaaa aaattcaaag aagtttaaaa
    12661 aactttggcc atatcatgta gaagaatcag aattagaaga ttgtcaaaat gaagaattta
    12721 tggagtcaaa agaatggagt attaaagata tagcttggat gttggctata ggttttgttg
    12781 ttacagaagt ttcaacaata cttgctggat atttcaattc aagttttagt agtgcagcaa
    12841 gaatactttt agttacaaca atttcaatta taattgctca attaaagcca gtaaagaaac
    12901 taaagggaaa tttagattta ggattatttg ttgcattatt ctttttatgt acaataggtt
    12961 tttcagtaga tataaaagag tttttaggtt caacttttac tataacactt tactgtttta
    13021 gtataatttt tgcttcattt gttttccatt taggcataac tagactttta aagataaagt
    13081 atcaatatgt aatactttct atagtaggag caattgctga tggtcctact tcagctttag
    13141 tagctgcttc tgcaaagtgg aattcgcttg taagtgtagc agtcgtaatg ggagtaattg
    13201 gtggagtatt aggaaattat gcaggtatat cagtggcata tgcaataaag atgtctctag
    13261 gattataggg gggagtttaa ttgataaaat ttaaattata catatctgga ctatacagtg
    13321 gaaatgtaat ctttgatgga gatttattaa ttgaaaagtt gaatcctttt acaaataaaa
    13381 tagaaagttt aaaaccaata agcaaagaag aaaatacata ttatttaaat cttacaaaaa
    13441 tagatttaaa gtctttattt aataattttg atgtatatac taaaagctta gtaaacacag
    13501 ataaagacac agttataaat aacttaggag aaacgcattc taaacttaat gaatatatat
    13561 gggttcaaag aaacaaaaag ttcccactag atataattat tgtagataat aaaatagtag
    13621 gttttatctg tttatctaga gaaacttgta ctatattaat tatggatgga tatgaagagt
    13681 atactgtatt aaaagaatgg gaaaaaactc ataaaaatga agagatatat tctataagat
    13741 ttggtggaaa ttatatgatt gatatgaaag atggtataaa actttctaca gatgtatatc
    13801 tccctgattt tgtagattct gccaaaaaag caccgacaat tcttatgaga actccatatg
    13861 gaaaagaaaa tgataaagaa atatattata aatatgtaca aagaggatat gctgttgtaa
    13921 tacaagatgt taggggaaga aatgaatcag aaggtaagtg ggaacctatg attcatgaga
    13981 gggaagatgg agatagtacc ataaattgga tagtatcaca ggaatggtca agtggaatag
    14041 ttggaatgct tggagcatca tatcttggtt atgtccaatg ggcagctgca tcttcaggca
    14101 ataagcatct taaagcttta gttagtatag taacatcagg aagccctttt attgatatac
    14161 caagaaaagg aggagctttt gtatctggta tgcttgcatg ggcatttatg gtatcaagaa
    14221 ataaagttga tagaagcaaa atggtaagag atgattggga tgatgtttta aacatacgac
    14281 caatagaaaa tatacctgtt gaagctcttg gatatagaat tgaatttctg gaagaatggt
    14341 taaagagagt agaaaaagat gagtattggg atttgatgga ctggcattta caaaaagata
    14401 aaataaatgt acctgcattg gttgtatctg ggtggtatga tgataatagt atgggaacta
    14461 cagaggcctt agatgttata aaggactatg aaaaaggaaa gagaaaggct attctaggtc
    14521 catggatgca taattcaaat actttaagag atataaatgg tatttcttta ggaaatagtt
    14581 cattaagata tgatttggat tataattatc tattatggtt tgataaatac ttaaaaggta
    14641 tagaaaataa catagatacg actgctccag tagagtatta ctctgtaggt tctaataagt
    14701 ggaagacaga agaaaattgg ccaataataa ataagataga taaaagtatg tatctaataa
    14761 gtgatggaaa tgctaacaca tctttaggaa atggtagatt agtatttgat aatgatttag
    14821 aagaaaagta tgatagttat atatataatc caaaagaccc atcagtacaa ctaattgata
    14881 tgtcagagaa tgaagttgga gttccaaata actacaaaga tttagaaaaa agaagtgata
    14941 tgttgtgtta tacatcagat gcttttagtg aagaatttac tgtaacaggt gatataaagt
    15001 tagagttttt tgcatcaagt tctgccaaag atactgactg ggttataaaa attatggacg
    15061 tggatttaga tggaaattca attaaattag ctgatggtat acttagtgct agatttagaa
    15121 atagttttta taagtcagag tttatggaag aaggagagat ttataagttt acgattataa
    15181 cttcaaaaat atcaaacacg tttaaagttg gtcataaaat tagactagat ataacgtcta
    15241 gtgctaaaaa ctttattttc caaaatagca acacgacaga gggatataat agtattgaat
    15301 atatagaagc aaagaatacg atttaccatg gaggtaaata tccatctaaa ttgatattgc
    15361 caatagagaa taagtaaatt tatatagatt aaatagtgag gaggtgtctc aaaatgaatt
    15421 ttttagttcg tgagacactt tcttttatac gaaatcaaat atattttcat catttcaaca
    15481 atgaaaaaga ggcttatctc tctgttttga gatagcctcc tataagctaa attgattatt
    15541 ttgtagctgc aacaacgctc atttcaacta acaaatgctc tctagcaagt ctagcttcaa
    15601 cacaagctct agcaggttca aatccttttt caacccaagc atcccaaaca gagttcatag
    15661 cttcaaagtc tttcatatct cttaaataaa tagtaacaga tagcaaatgt tgcttatctg
    15721 aaccatattt gtttaataaa tcttctattt tagctaaaac ttcagttgtt tgttcaatta
    15781 ctcctccttc agcatgagtt tgtccacata aataaactgt attgttatga actacagctc
    15841 tactcattct tccagttcct tcatatctct ttatttccat attttaaata cctccatagt
    15901 taaaaaatct attaaaaatc taaataataa attaaaaaaa gtctatacta tataaaatta
    15961 tattatatat tctgataata atccataaaa acatataact ttttagctat aattattatt
    16021 gttattaatt gaaaagattt attagttact tgacatagtt tacactttta attatagtat
    16081 aaattggaat atttagataa ttattattta atgtataagc taagttttta attaatatgg
    16141 ctttatacat ttatatagaa aagaggtacg tatgagtagc acaatcaaat caaatcgaat
    16201 aaaaggtata atgtttataa tagcatcagc gcttggcttt gcaatgatgt ctgcattcgt
    16261 aaagctagct ggagatttac caagttttca aaaagtgttc tttagaaatc tggtatcagc
    16321 tataatagct ttttggctta tcataaaaca taaaggaagt ctaactggta aaaaagaaaa
    16381 tttaaaagta cttttatata ggtcaatatt tggaactcta ggagttatat ttaactacta
    16441 tgcaattgac aagttgatgc tttcagatgc taatatgcta aataaaatga gtcccttcct
    16501 tgtagtaata ttttgtgcaa ttgctctaaa agaaaaaata aatgtaaagc agataggagc
    16561 tattataatt gcatttatag gagctttatt cataataaaa ccaacattta gtgtagaagt
    16621 aattccatat cttggaggaa ttgcaggagc tgtatttgca gcaatggcat atacttgttt
    16681 aagagttctt ggagatagag aagattacta tacaattgta tttttctttt cagttttttc
    16741 attagttact gtagggccaa ttgcttttgc agtatatgaa ccaatgaccc ttatgcaact
    16801 tatatatcta ttactaggtg gagtatttgc aagtctaggt caatttggaa taactctagc
    16861 atataaatat gcacctgcta aggaaatctc tatatttgac tatagtaata taatcttttc
    16921 agcaatttta agtatattct tattcaatgt ctatccagat atgttaagtg ttgttggata
    16981 tttaatagta ttttcggcag cattttatat gtttttatac aataagaagt tagataaatt
    17041 agacaaagaa aagaagaagt aaactagata aaagtgtttt tataatttgt atttaatatt
    17101 taagtctttg tattaaaatt aaagaaaatt taattaaaaa tgtttggtat attaacttat
    17161 aacagcagtt aaaattgaaa aattataact gctgtttctt ttattaaaaa tatccaaatg
    17221 tctgtaaaaa tagactataa tagagtagta taaataatat aaaggggata aattaaagta
    17281 tggaaaaagt gaaagttgct gtgattggag caggtaatag aggaacgtat gcatatgctc
    17341 catatattta tgaaaattct gatgtatgtg aaatagttgc tgtagcagaa cctaaaaaag
    17401 gaagacgaga actatttaca caaaaatata acctagatag taaaaatgta tttgaaactt
    17461 tagaagactt ttttaaacat gacaagatgg cagatgcagt aataatagct acaaatgatg
    17521 atagacatta tgatgtagca aaactagctc ttgaaaaagg ttatcatgta ctattagaaa
    17581 agccaatgtc taatagttta gatggtctgg tacatataga tgacttgtgt gataagtata
    17641 aagataagat ttttatgatt tgccatgtac tgagatattc tccattttat aataaattaa
    17701 aagaaattgt agaaagcaaa aaattaggag agctagttag tatacaatat aatgaaaata
    17761 taggatattg gcattttgca catagcttta ctagagggaa ttggagaaat agcaatgaaa
    17821 ccagcccttt aattttagca aagagttgtc atgacatgga tattttgtta tatttagtag
    17881 gaagtagatg caaaaaaata tctgcatttg gaagcttaaa gcatcttaat aaccaaaatg
    17941 ctagtggaga gatggcacaa aattgtcttc agtgtttagt tgaaaaaaaa tgtccatatt
    18001 cagcaaagag aatttattta gaaaaagata gaagtataaa tagagctgtt cacataaatc
    18061 caactgaaga gaatctattg aatatactta agacaagtcc atatggaaga tgtgtttaca
    18121 gatgtgataa taatgttgta gacaatatgg taaatatact acagtttgaa aatggagtga
    18181 cggctacttt taatttatgt gcatttacta aagaaaatgg aagaactata aagttaatgt
    18241 ttagccatgg tgaagttggt ggggatttaa ataaaaatga gataagaatt aaagaatttg
    18301 gaaaaaacga agaaatagtt atgaatccat caaatcaaca gaatatggta gaggaatatg
    18361 atagaaatct tatagctgaa tttattaaat tagtttcaaa taaagaacta gaaaagggta
    18421 gagtagcagc aaaagaagct atacaaagtc atgtaatggc atttgcagca gaatattcta
    18481 gggtcagtga tgaagtagtg tatatagaag aattttttga tagtgctaag caaatgacta
    18541 aggagataga agaaactata ttttaattaa taaaaattta ttaaataaaa taaagataga
    18601 ttaaataata gaaatacgaa aatatgtgtt ctattaagga atctatcttt tttattaagg
    18661 ttttttctta tccctatcaa aaaaatccat acattctgat aaaagataag aaagtaaagc
    18721 gaataagatt tctgtactta aaggaatcgt tactgaatcc ataaaggtat caattatgtt
    18781 catagctaga aaagttagta taaaatcaat tataaattcg caaagtttat atattgagta
    18841 tggtaatttg tttacatatc tgaatatatc taaaatagta gtgcatagaa aatcaattgg
    18901 agtggttata caaaaatata ttaaaaagaa aattaataca gctccaagag atttatattc
    18961 aaatcctaag aatgataaga agccaattcc tataaccgtg aaaatacaaa aagttatacc
    19021 aaaaaaaaca gcaaaaaata aaaatacaga aataaattcg cctaaaggaa tccttctttt
    19081 attacttatc ttaatcaact ccttaattta tgatatgaaa tttatataaa taataacatt
    19141 cttaaaaatt cataatagtc ctctgtttca aatagtactg tttgagaatc tatttttttc
    19201 atatttggat aaaaagaggc cttaaaagct ttataatgtt ctctaaattt tatttctatc
    19261 ttaaatttat ctggcaattt tattaagtgc ctagataaat ctccagataa agcttttttg
    19321 acaccttttt caattttttt tagtgcaagg tttggatgga ttgaaattga accatttcca
    19381 attccttttg acacaggaac agtgactata tttggattta gtttttttgc actctcacaa
    19441 agcatttcat ctccacttaa aaatgctaca ggaacattat aatatgcagc aatataagca
    19501 tttatagtaa attcagatgc tatttcatca tttattttaa aatagtcata atcaatatcc
    19561 atagtatgag aaagtggatt accattacaa gtagctccag agtgatagcc ggtgaatata
    19621 catgcatcaa agctttcatc aattcctgcc atcataacta gtgggtcttt ggtccatcct
    19681 ctaagtattt tgacgttttc aggtaaaata tttggattaa tatttctacc actgtcatgt
    19741 gcatctttaa caagtatttc atgtacacca gattgagtag ctccatcaca tgctgctgtt
    19801 atctctttac tcatttgcgt tctatagtgt tgcgaaaatt cacattcaag ttttgtttca
    19861 tcccaattta caataccaca agtaccttct atatcagcac ttaaatacaa tttcataata
    19921 tataccccct tatgtttatt attgatgata ataacagata acagaaaata cataatcaat
    19981 aaagggcata aaaatattat gtcttactat taactattat taaatctgta actgaaaaat
    20041 aactaaattg gtataaaaaa atataacact atgtgtcatt tatttatata gttttttata
    20101 tttgatgttt atataaaatt gtaacatata agtggttaca tttactagtt taacatagtt
    20161 aaaatagtta ggtattattt ttaagttaaa ttgattatta taaaatcgca gaataaaaga
    20221 aaaatttaat ttattaaaat gcatgaaatg ttttttcctc aacataatgc taaattaaac
    20281 tcaaaaagaa ataatagaaa aatcttatgt agcttagaaa gaatgtgaat gatttaaggg
    20341 aaaaatagaa atttattata taatattagg ttgtattata taatacagcc taatattata
    20401 tgtatatgtg acataaaatc ttggtaagtg taaataaaat aaatgtggaa aacaagttaa
    20461 tgaaatcaag aaaaaagtaa tttaaaattc aatttcatct tcccaattaa attctcctga
    20521 tttttctaca ctcttaggcc agtgactaca ccatttagga acggacggat tttcaaccct
    20581 atcaacgctt ggagtatatg gctttaagtc aataacagga ctattatcat tagcatcaat
    20641 atatggtatt tttattatgc cattttcatg gtcgatattg ataatttgta cagcagtcaa
    20701 tgctattgga ttgggtctta taggtgacct tgttgcaaat attcctataa cgtctggtcc
    20761 acttttataa ggttttgggg tttctaaaat acttcttagt tcaggattat caaacccatc
    20821 agcccaccaa aatacattta aatgactgaa atcgtcgagt tcatttaatg caggtacata
    20881 ctttttgtct aatttaataa agacctcttc gccttgaata catattttac ctataggatt
    20941 tattaatatt tctttcataa tattacctcc aactatttaa tttatcattt gtaattttag
    21001 ggtataacat gacatcatgt gagggtcaat ataaaataat aaataaaaat attaatagtt
    21061 agaatgataa tacatttttt acatggagca tgacaccagt aataaggcaa tcttcgtcaa
    21121 tatcaaaagt agaggtatgc aaaggggaag taatgttttt ttcacgattt gcacaaccaa
    21181 ggtggtaaaa agctccttca cagtgtctta gaaaatacga aaagtcttca gcaccaagag
    21241 tgggcgagtc tttaaggata atattttctt caccaaacaa ttctacagca ttttgtttga
    21301 cgtaatctac tagatggttt gaattgataa gagctggata accttcctca atttcaacag
    21361 aaatacttcc tccaaatgtt ttacaagtaa aatcgcaaat ttctctaatt cgtttttttg
    21421 aaaactctct agtttcagga gtcaaggttc tcaaagtacc ccttataacc actttgtcac
    21481 aaactatatt ttctttgatt ccaccttcaa tctttcctag agaaataacc acagaatcag
    21541 ttggatttgt gtttcgacta acgattgtct gtagagctgt aataatatga gcagatataa
    21601 caatagcatc aataccttga tgtggataag ctccatgaca ttttgaacca tgtacagtta
    21661 attgaagtgt attggtggaa gcatttagag tgttgtattt taattcaatt agatttttgt
    21721 tgatattggg atttacatgg agtccaaata tatattttac atttggtttt tcaagacatc
    21781 catcttctat cataagttga gccccaccaa tagtttcctc agcaggttga aaaaagaatt
    21841 ttacatttac atttaagtaa tcctttaatt taaatagtat attacaagta cccagtaaaa
    21901 tagcagtgtg tgcatcatga ccacatgcat gcattttgcc aatattaata gatttataag
    21961 aataattaag ttcttctgtt ataggtaagg catctatatc agctcttata gcaactgttt
    22021 caaagtttgg actcacgtta atgtatgcag ttatccctgt atgattctta tattcaatat
    22081 aatttatacc tatttcattt aagtatttaa taattttttt cttagtttga aattcttcta
    22141 atccaagttc tggagtctta tgtaaatctc ttcttatatt tataacccaa tcatttatct
    22201 catgacaaag agtttttata ttaagcataa aacacatcct aactatatta aaaatttatt
    22261 tttcttcatt aattatatta ttttatgtaa atcattatta tgtatcaagt acaaataaaa
    22321 tatcttataa ttatattaga gatatttagt atggagaaga atatatcatt ttataagaag
    22381 aggtgtttaa atgttaggaa taattgcgcc atctgggcca ttaagaaata caagtttgga
    22441 ggaaataaag tttaatttag aaagttatgg ttacgaagtt aagttttctg aaagttgtag
    22501 tttaaattat aaaggttatt tagctggtaa tgatgatatt agagcaaggg atatagaaga
    22561 tatgttttta gataaagatg tagatataat tatgtgctta agaggaggtt atggaacaac
    22621 aagaatattg gataaaataa attatgatat aataaagcaa aatccaaaac catttatagg
    22681 tttttctgat ataacaggtt taaatttagc tttctataaa aattgtggtc ttttaccata
    22741 tcatggaata atggcagcaa atgtaggtaa atggggtaaa tttacctata aatcattggt
    22801 aaatgcttta gaatttaaag atgaattata tttagaaaat ccaaaagaag aaaagattta
    22861 tactgtatgt gaaggtaaag cagagggaat aataatggga ggaaatttat ccttaataat
    22921 tgccacaatg ggaacaaaat atgaaataga tgcaaaagat aaaattttgt ttattgagga
    22981 aattggagaa cctcaatata aactggatag aatgttaaca caactatatt cttcaggaaa
    23041 acttgaggaa tgtaatggta taatttttgg agattttaag gattgtattg aagaaaatga
    23101 tttgatggaa ttactaatag aatttgcaaa taaggtcaat aaaccttcta tatataatct
    23161 tcagtcagga cattgtatcc caatgataac tattcctttg ggcagaatgt gcgaattaga
    23221 tgctacagaa aaaatcgtta aattaaaaaa atagtgtgta tataaatata atagaaatat
    23281 ggtataatct tatacaagta aaaatttagc taggaggatg aaaaatgtct gatgatagaa
    23341 taatagactt taacgaacta aagaacaaag ttaaagattc tgatgtagat aaatttgagc
    23401 aatacatata taacttatat ttttctgtta tggatggaaa gatgtctatg gctgagtttt
    23461 caagaaaaat attcgattat atgagagata acaacatatc tcaagaaaaa tttatgaaaa
    23521 tacaaaagca atttatggag agatatggta tggacactga agaagtagaa aaacaactta
    23581 gaaactttgg aatcgaccct tctacagcag gttttatgag taataatact tccagcaaag
    23641 tatctacaga agatttagag tcatttaaga agagtgcagg attttatgag aaatatggag
    23701 aaaaaatcca accaaagagt tgtataacaa catttataaa aaatgactta aatgatatta
    23761 atgtaataat cgaccaagaa aagataatgc tttgtagtga tagaaaaata aatcttatgg
    23821 attcagaact aaatgagttt ttattagaat ataagaatat gtttaataaa aaaataaagg
    23881 tagtaatgtg cgaaactacg aataagtatg attattaaaa aatgaattta aatactgaag
    23941 tagtataaat taattgtact acttaaaaat aaaaatcgca atttattatt ccttataaat
    24001 ttgtataagg aatatctaaa ctgcgatttt ttatattgtg aagattagta ataatagata
    24061 gtgttttaaa ttgtaatctt ctcactaaaa ctgtgttttt ctattagaaa ttttattaaa
    24121 aataaatatc tgataattgt attatatggg atgtaaagta ttagaagctt aacgaatctc
    24181 ttttaataca ttaactccat tttgaatcat actgttgaaa aatactaagt ttaaaatctc
    24241 agcagggtga acgttaggaa tatcataagt atctactaaa ttcataggta caactatatt
    24301 tttttcaata ttatgttgat taaaatatga ttttagagtt attgcaaatt gatatataca
    24361 tatatctgtg caatcgccaa ctataattat attatctata ttatcaaaat ccaaattttc
    24421 taatgcaaaa aatccattag tagaattttt aggaagaatt tgtaggtttt ctatcgactt
    24481 aagttcatca accaattcac tttctacatc attttccaaa cagtgaacag gataactcaa
    24541 aagttctatt gatttaggag tatgagaatc agtaaatgct attactctat ttaatttgtt
    24601 tgatattttt tttgtaaaca tttcaattgg ctttattaaa gactctactc taggagagta
    24661 taatgcacct tgtcttgcaa agccattatt aacatcaatt ataaataaag cagttttact
    24721 caaatcataa ttttctattt tttctattgg tagattatct aaattactct ttaaagcttc
    24781 aagttcattt aataagttat ccatagttat acctccaaga taaatttaat aaaattgtac
    24841 atattaatta tataaaagtc aaggagtaca aactattgta taattagctg tattgttttg
    24901 agtgtaaaag atttgaagca atatctggat agttagtaaa aataccatct acattgagtt
    24961 ttagacagtg tagtatatca ccctcttcat ttacagtata tacattgaca ccaagtagat
    25021 ttttgtgaca aagttcaatt agagattcat ttactaaaac tagaggtgga tgaagataac
    25081 tattacccag aaatttagca aaattgacta cattgtttat aggtaaataa cataataatc
    25141 ctgtttttat actagggtct aatttatgaa agttttttaa actattgctg ttgaaactag
    25201 atataagaac tctatttttc atattatact ttaaaataag ctctaaaacg tctttttcta
    25261 tattgggata gtttattttg tcagttttta gttctatatt taaaataagg tctgtaggtg
    25321 aaattagaat taatacttct tctaaagttg gaattttaca ttctttattg ctttgatatc
    25381 cttcaaaaga tgagtttaga gtttttaatt ttcttaaagt aaagtctttt acaaaaccat
    25441 gtccattaaa agttctatca accttttcat catgaataac tacaattttt ccatccttag
    25501 ttctgtggac atcaagttct attccatata tatccatatc caaacatttt ttaaaagcta
    25561 gaatagtatt ttctggatac tttccactaa accccctatg agcataaata ttcatttata
    25621 tggttccctc ctataagtat aacttatctt ataaaaattt aattaatata atatatcata
    25681 ttccaaaagg tattgttata tattagttaa ataactattt gtaatttata tattatataa
    25741 atgtagaaaa ataaaatcat atatgtgtta taatatattt taaacattgt aaatattacg
    25801 aatttagtag tcataataag tgtaatttta tgcaagctca tgtttttaga gcttgcatat
    25861 gtattattaa tatatttata tattgtgaaa ataaaattcc aaaagaattt tttctataga
    25921 cttagtagta agggggaatt ttgatatgta tgaagttaat ttagaacgag ctatgaatta
    25981 ttggatggat ttgttagatg attatacgaa tatagcaaaa attccaaatg atttttttta
    26041 taaagattgt tctgatgaag tagtagatga ggtttttaag atgaatattg agaaacatga
    26101 aaggttaatt caatattcta aagtgaacaa tattagcata gactctatca tagaaactat
    26161 atatggcctt atacttcaaa agtatatata tgaagatgat gtagtttttg gtaaaacaat
    26221 gaatataatt ccaataagag taagagttag acaaagtgaa aaatttatag atgttgtaag
    26281 gaagctaaaa aggcagtgga gcaggtcttt agagtatgga tactatttgt tatcagatat
    26341 agagaataaa aatcatctaa atactaagct tataaacaca atatttatag ctaaaagttt
    26401 tgatttatca tatgattatg acatagaaaa aaaaggatat gatttatctg taatagtata
    26461 tactaagagg gcacttaaaa taaaaataaa gtataaacca tctatatata aaagagaaac
    26521 tattagaaga attttaaatc aatttgatta tataatagag caaataatag aagataatga
    26581 tattttaata gaagatttag aatttgtatg taaggaagaa gaaaaagtat tattggaaga
    26641 ctttaataat aatcaatatg aaataccata tgataaaagt tatgttgatt tatttaggga
    26701 gcaagttaag gtaacaccta gtaatatagc tgctagggat gaaaatagaa agttaacata
    26761 taaagaattg gatgaagtaa ctgataaact tgcaggttat ttaaatcata taggagtgga
    26821 aagtgaagac atagtagctg taatgttacc aagagatatt aacataatta ttacagcaat
    26881 agggattatg aaatcagggg gagcattttt tccaatagac acaagtaatc cagaagaaag
    26941 attgaattac cttcttgaag atagtaatgc aaaagtagtg ataactacag atgaactcaa
    27001 aagtaaggtt gtaaatgaga ataccatagt actcgatata aatgatgaag aaatgtttaa
    27061 gcttggatat gaattaactg aaaaaataac atcatcaaat tgtgcatata cgatatctac
    27121 ttcagggtca actggtaggc caaagaccat agctatagaa cataagagtt tagtaaatat
    27181 gtgttattat agcgtaaaat ctataagcgc tacagaaaat gatatatgtg gtatatactt
    27241 aagtttcagt tttgatgctg taatgaaaca actattccct tatttgcttg taggtgcatc
    27301 aatagatatt atgcctgaag aagctaaatt tgatgaatat acagtaaatg aatactgtga
    27361 ggaaaatgac ataactatac tagcattgcc aacagcattt gcaaggttat ttatacaaaa
    27421 ttgcaataat aattcactta gagttgtaca gaccggagga gaaagattaa aaggatatag
    27481 aaagcgtaat tatgaactat ataatgagta tggaccaacc gaatttactg ttgtatcgac
    27541 ttcttttcat gtggacagag aatatggaaa aataccaata ggaaaaccta tatttaatac
    27601 ttatgcatat gtattagata aaaagaacaa actatgtcca attggagcac caggagagct
    27661 atgtctatca ggcattcaaa tatcaagagg atatttaaat aaaaaagggc taacagagca
    27721 agtatttgta gagaatcctt acaaaacttg tgaacataat aagttaatgt atagaactgg
    27781 tgatttagta agatggttag atgatggaaa tttagattat attggaagga tggacaatca
    27841 ggtaaaaatt gatgagttta ggatagagct atatgagatt gaaaatataa taaataatat
    27901 tacagaaatt aaaagtgttg tttgtatatc gagaacaaat gatgatggag atatgtatat
    27961 atgtgcatat tatgtaattg atgaggaaga tagtggtaag ataaatgaaa gaactattag
    28021 agaatattta aatgagcatc tacctccata tatgattcca acaataataa tgaggataga
    28081 taagatacca gttacaccaa tcggaaaagt aaataaaaga gcattaccag aataattttt
    28141 tattatgata ggtgtcataa agtgatttca gacatttctt tatgacactt atttttataa
    28201 aagaatcaat ttgcataaaa acaagaattt tctgtatata tttactatgg tgaaaattta
    28261 tttataagaa tgattattgt attttttata cttttccgtg ttttaaagca ttttttttac
    28321 aaaattaatt ttacattaaa aaattttttt attttatatc ttaatagttt ttagtagaat
    28381 tgaaaaataa aaagaaaatt agcataaaaa tatctaaaag tagaaattaa taatataggg
    28441 ttaaatttta gtagttttga ctcgctattt gtaaaataat aaaaagttaa gttttatatg
    28501 tatatatgtt ataatatata caaattgaaa aggggtgaaa attttatgaa caaaaaatta
    28561 cctaagggtg cttatggtga ggttagtgga aaagattacg ttccatatat cacagataag
    28621 tccagaaccg gtggaaatgt agctgtactg attatcggta ttattttagc tgccattttt
    28681 gccgcatcta caacttactc tggaatgaaa gccggtctta cagttgccgc aggtattcca
    28741 ggagctataa taggttctgc ttttgttgga gcatttgctc gttcaaaggg tattcttggt
    28801 aaaaacttaa tccaaggtat gtcaagtggt ggagaatctg ttgcaagtgg atttattttc
    28861 gtattaccag cagttatatt aattggaagc caaattacat tctttgaagg cttagctgtt
    28921 ggagttggtg gagttttatt tggtatagga attgctgcta tagttcacaa ctatctaata
    28981 gttgaagaac atggtaaact tatgtaccca gaatcaatgg ctatatctga aacacttgtt
    29041 gcttcagagg ctggtggaga ttcaattaag tacatgggaa ttggatttgt aataagtgga
    29101 tttattactg tattaactgg ttcattctta aatgtagcaa acaatgtaat gagtcttgta
    29161 ggaagcaaat tctacaaatg gaaatttgat attgaagtaa atcctctact tttaggaatt
    29221 ggatttatag tagggcttga agtttcttta acaatgtttg caggaagtat tttatcaaac
    29281 tttggtatag cacctttaat aggatacttt acagatatgg ctaaagatgg agctatggta
    29341 tggaataatc ctgcaatgcc tcttaaccaa atggatgttg gagcaatatc tagtagttat
    29401 gtaaaatata taggtgctgg tatgatgctt tgtggaggta ttattggtgc aataaaactt
    29461 ataccaacta taatcgcttc tataaaagaa acacttaaag ctaaatcaag tgctggagaa
    29521 ggagaagaag gttcatctat acaaatgata cttttattag gtggagtagt tattggattt
    29581 ttagcagcat tcttaatttc tggtaacatt gttatggcaa ttattggagc tatcatatct
    29641 cttttattat ctttattatt tgtcatagta gctggtcgtt taacaggaac aatcggaaca
    29701 tcaaatcttc cagtttctgg tatgactata gcttctctag ttattgtaac attagtgttt
    29761 gttataatgg gatggactga tttagaagct aataaatcat tacttttatt tggttcattt
    29821 atagttgttg ctattgctat agctggaggt tatactcaat ctcaaaaagt aacttatatt
    29881 attggtggta gcaaaaacga aatgcaacgt tactttacaa ttgcaagtat agttggtgtt
    29941 atagttgttg taggagtaat tttattactt tctgaccaac ttagagcaac tggtgataat
    30001 gtacaatttg cattaccaca agcaaactta atgtcaacat taacttctgg tattatgtca
    30061 ggtagtttac cttgggttat gattattgtt ggagtattta tggcaatagt tctatatgca
    30121 ttaaatttac caataatgac tatagctata ggattctatt taccaattgc aacaacttct
    30181 ataatattag ttggagcatt aattcgttta tttgttgaat tagtatctaa aactgaaaaa
    30241 gaaaaagaag ttaaagtttc taatggtata agtttatctt ctggtcttgt tgctggtggt
    30301 tctatcattg gtttaatagg tataatatta caagtaactg gagttgtaac tccaaaggta
    30361 ccaagtggat ttgctgcaac aaattcaatg gcaattgctt tattagtagt tttagttgtg
    30421 cttacagcat tacctattgt tttatctaaa gtaaaaaata atgagcaaga gtaatgatgt
    30481 tttagatatt gtatttaaaa ttactgatgg acttgaatat gaagtagctg aaaatagagt
    30541 tgttactata cttgaaaaac aggaccataa aattcaaaag ttttttagaa agttaaaagc
    30601 tagaatacct aaatacaaaa aaatagaact agatgaatat tccagttttg tttttttaca
    30661 aattgatggt caaaggactg ttagagaaat aggcaagaac ctagaagcta aatatggaga
    30721 agaatctcat ccactttatg aaaggttgtt agtattttta aatcatattg aagttaattg
    30781 tcattatatt gaaagactgg atatatagat ataataagaa ttattataga tatattagag
    30841 catataaatt agaaggtaat tatgaaagag gctgtctcaa aatagagata agcctctttt
    30901 gtattgttga aatgatgaaa atatatttga tttctcataa aaaagagtgt ctcataaaac
    30961 taaaaagttc attttgagac acttccttta aaatatttat aaattacaga tttaaagcaa
    31021 aacttattaa acttaagatt attttaatat tacttattat tagctatttc atcagctaga
    31081 ttatttaaat attcccatct ttcatatttg tattcaagtt ctttttctag tttagatttc
    31141 tcatctaaaa tttcttgaag tttagtaaaa tcagttgaaa attttgcagt actttcatca
    31201 agtgttgcaa ttttagattc gagtttttct atatcagagt caatattttc aaactccctt
    31261 tgttcattat atgagaactt aagcttttta tcatttttag gtttttcttt ttttagggta
    31321 gtagagtcat cttttttaac tgattccttt ttagattctt caaattcaat tccttgaatc
    31381 tctctataaa taagataatc actgtaatta cctgtataga tatgaatttt tccattacct
    31441 tcatatgcaa atattttatt acaaattcta tctaagaaat atctatcatg tgatactgta
    31501 actactacac ctttaaattc gtctaggtat tcttctaata ttttaagcgt ttcaatatct
    31561 aaatcatttg taggttcatc taaaagaagc acattaggag ctgtcataag gattttaaga
    31621 agatgtagtc ttcttctttc accaccagat aatttaccta tcaaagtata ttgcatagta
    31681 ccattgaaaa ggaatctttc acacatttga gaagctgtaa tcctagtacc atctgaagtt
    31741 tctatatagt cactagcttc ttttacatag tctatagccc tcatatcaat attcatatga
    31801 gagtcgtctt gagaaaaaca acctatttta accgtttctc caattgaaat atatccacta
    31861 tcaggctcta tttctccatt aagtatattt ataagggtag atttacccat accatttttg
    31921 cctattatac caattctatc tgtacgagtt aaagtgtatt ctaaattatc tataacagta
    31981 ttttcttcaa atgatttaga tatattgtgt atttctatta ttttatttcc aagtctactt
    32041 gatgcaacag atatatcaag attttcatca ggtttaaaag aatctctatt tataagttca
    32101 tcaaatcttt gtaaacgagc tttttgttta gtgctacgag cctgagctcc tcgtcttacc
    32161 cacgcaagct ctgttcttat taaattttgt cttttatctt caatacttgc ttctagcgaa
    32221 agtctttcca ttttcttttc caaaaatatt gaataattcc catcataact gaataatcta
    32281 cctttgtcca gttcaataat tctatttgta acacggtcta aaaagtatct atcatgagtt
    32341 atcataagca atgagccttt tctggaatta agatattcct caagccaatc tatagtatca
    32401 ttgtctagat ggtttgtagg ctcatctagt atcaaaaact cacaaggtgt aataagggct
    32461 gatgccagag agactctctt tctttgccca ccagaaagtt ctgctatttt ttgattaaag
    32521 tcatttatac caagctttgt aagtactgac ttagcttcac tttctaaatc ccaaagattt
    32581 aaggcatcta ttttttcgtg tactgataat aattttttat ttaattcctc tgtaaagttt
    32641 gaattaagtt tttctaggat ttcttgatat tcaccaagta gctttaattc ttcagaagtt
    32701 ccattaaata cttgttcaag tacagttgaa ttttcattgt aagttggatt ttgaggtaga
    32761 tattctatcc taataccatt agtttttgtt atattaccag tctcactttc ttcaactcct
    32821 gcaataattt tgagtaaggt agatttacca gtaccattta caccaataag tcctattttt
    32881 tccccttcat ttattccaag agatatattt tctagtaatt ttttttcaga atagctttta
    32941 cttatatttt ccaatgtcat taaattcata taattcacct ttatttctaa tctattattt
    33001 ttcaaaatgt tgataatctg ggtttttcca atagccaccc caactccaac ctctgcttac
    33061 aaaagcatta taacaatcat cacctttttt tatcattcca aattttgtgc tagacctatc
    33121 agcataaact gtacttactt taggtgtaac atcatttcca gaaacttgag gattctgtaa
    33181 tgggtttata tctatagcac gtcctttacc atgatttgaa actacatttg taccagctat
    33241 agttctataa caaaatgatg aggtattatt atcagacata gatttttcat ctacagcatc
    33301 atagtcatct attaacttaa ttttttcaat tggatatttt ttttcgtata attccttaaa
    33361 aatatccact acttctggag ctagttcact gtttactatc atttctcctt gatgagtttt
    33421 ttcatcaaat ccataatatg taagttttaa ataagataga ctatcaaagc ttataggttc
    33481 atcagtaggc atagatttac caatcatttt attttttata ttatctggta ttgaagaata
    33541 tgaaaaagta ggcttgatat tattatcttc ctgctttttg gtagagcttt gattattttt
    33601 agaatcatct gttgtattat ttttattttg catagtttct tgactcttta tattaccttt
    33661 tttgttggga ttagagaaca gtatctcata tatagataat aaagcaagaa ataatacagt
    33721 tattacaatc gttaatttta atttattaat ttttcttcgt tttgactttt tactcatatt
    33781 atcccccagt attagattca tataaactat atatattata cctcataaaa ataaactagg
    33841 ctaacaatat ttagttaacc tagaacattt catttatagt ttctccaatt atttttattc
    33901 caatttcaat gtcttcaaaa gacaacctag aaaatccgag tcttaaagta tcaaaaccag
    33961 agttatctgt aaaaaataaa tctccaggca taaatataac atttttttca taacattttt
    34021 ctaataaaac tctagtatct atatttttta gttttataaa tatatgcaaa ccaccatcac
    34081 ccaagatata ttctgttttt atatattttt ttatacactt atatgcaaag ttaaatttgt
    34141 ccccatagaa tttacgtatt tttttaatgt atttttcaaa acctccatta cttaaataat
    34201 caaataaaat tccttggtcc aaaaaggata catgtatgtt tctgcacctt tttacacttt
    34261 ctagcttatt gattactttt ttatctgcaa atatccatcc aattctcatt ccaggaaata
    34321 aaatttttga aaaactacca atatatacga ccccattatt catattatct agggaacaga
    34381 taggaaatat atgagaacta ttgtagagta attcttcatt aaatccatct tctataattg
    34441 ctatattgta ttttttcata agattataga atttgtacct attttcagga gtcattacaa
    34501 ttcctgtagg attgtgataa gagggtgtaa tgtatgcaaa ctttatttta tttttatact
    34561 taactaaatt gtcttctagc atattgaagt ccaaaccatc ttcatttata tctacaccta
    34621 agatattgag gttatgagat ttcataattt ttatagcagt attatgagta ggattttcac
    34681 atattatgta gtctttttct tgggtaaaag atgacataat aatatcaaag ccttctgtga
    34741 atccatttgt tattagtata tctttattac ttatatcagc acccttattt gtcatatatt
    34801 ctagtaaata gtcaataagg ggcttataac cttgtgcata tccatagttg agtaatttat
    34861 gtccttctag tgaaattcta tttaaaaagg atttttttaa ttcttctatg tcgaataaat
    34921 ctccatctgg agaaatactt ttaaaggaaa tcaggtcaga gctccaagga atttcacttt
    34981 ttataatgtc caattcattg gcttttttag agtatgtatt ctctaaacaa tcccagttta
    35041 atgaccatgt agtatttgaa gacttattct ttgatttaac aaatgtacct tttcctgaaa
    35101 tagagtagat taatccttct gattttaatt cttcataagt taaaactaca gaatttcggc
    35161 taacttgtaa aagctgacta agctcccttg tagaagggag cttactattg tctggaatta
    35221 gtccttttga tatcatatca cttatataat ttttaagttg tatataaatt ggttcattat
    35281 catttagttt tagatttgaa aatatcaaat taccacctca aattatttca tctaatttaa
    35341 agagtattct ataagtttat ctaatagttc actgtactta atacctctag cagcagcact
    35401 tctaggaatt aaactagttt gagtcattcc aggaagagta tttagttcaa gaacatatgg
    35461 aataccttca cttataatca tatcaactct tacatatgct ttgcaattaa aaattttcca
    35521 acaagtctca gaaatttcgt ttactctttt ttgaagttct tcatctaagt aaacaacttc
    35581 ttcctttgca cctttttcaa ctgagtactt agcttcataa tcaaagaatc ctgaatcaga
    35641 ttttatagat attgtaggga acacttctcc atttaatatg aatgaagtat attctccacc
    35701 tggtatatat ttttcaatca ttacaaattc atcaacttcc agaccttttc taactgcttc
    35761 ttcaacttca tccttagaat gaataaagaa tgtggctaca cttgaacctc ctgagtttgg
    35821 ttttataaat actggataac ctatattatc tatttcatca tagtctattt catcaactga
    35881 ttttactaaa acccatggag cagtaggtag gtttgaatct ctaagcattt ttttagttat
    35941 atttttatcc atacacatcc cactgcaaag aggtccacat ccagagtatg gtatatccat
    36001 agtctcaagt attgattgta tacatccatc ttcaccaaat tttccatgta aagcaagtat
    36061 tgcaaaatct atatcttctg gtatctttgt aaatatatct tttttatcat ctattattac
    36121 ttttacaact tcatatttat ttttatcaag gttattatat atctcttttc ctgaatttaa
    36181 agaaacttct ctctctgatg atattccacc cattataact gctattttca tattatacac
    36241 tcctatttta atttttatta ttttaaagta aactttttat ttacatactt aattatatga
    36301 attaacagtt gaggatgaaa gtaccactat atacgaagtt tatattatgt gtggttttag
    36361 aataataatc tagtttaaag atatgtttaa atgatttttt aactttattt atgttattat
    36421 ttttgctaaa attattgtta tgaacttaag actagtctta atggctataa atcaaggagt
    36481 gataaatttt gaaagctgaa ataataagtg taggaacaga aatattattg ggagatatag
    36541 taaatacaaa ttctcaattt ttagctaaag aacttgcatc attgggaata gaagtatacc
    36601 atcagagtac tgttggagat aacaaacaaa gacttttaga atgttttgat gaaagcttaa
    36661 agagaagtga ttttgtaatt actacaggtg gtcttgggcc aactggtgat gatatgacaa
    36721 aagaaactgc tgcagaatac tttggtcaga aattagaact acataaacct tcactagagg
    36781 ttttggaatc tttctttgtt aaaacaggga aaaaaatggc cgaaaataat atgaaacaag
    36841 tgtactttcc aaaagatgct atagtactaa aaaataataa tggtacagct ccaggggcaa
    36901 ttttaaaaaa agatggtaaa tttataatag tattgcctgg acctccaaga gaaatgaaag
    36961 ctatgtttaa tgagagtgtc aaaccttact tacaacaatt cacaaatgaa atgctagtat
    37021 ctaagacttt aagattatat ggaataggtg agtcaaatct agaattagaa attctcgata
    37081 taattaatga gcagactaat ccaacagttg ctttatatgc aaaagaatta gaagttacaa
    37141 taagaataac tgcaaaggct gaaaatgaaa gagaagcttt taaattaata aaaccagtag
    37201 aagaaaaaat aaaaagtaga gttggtaaat atgtatatac tgaaggagat atttccatat
    37261 ctgaagggga gactgcttta gaagatgctg tttctaagct acttgtagaa aaaaatctaa
    37321 caatagcagt ggctgaatct tgtactggag gtttggtttc ttcatctctt ataaactatc
    37381 caggtatatc ctctgtattt cttgaaggtt gtgtaacata ttctaatgat tctaaaatga
    37441 aaagattagg agtaaaaaga gaaactttgg aagagtttgg agcagtaagt gagcaaacag
    37501 ctatagaaat ggctgagggc gttgctaaag gacttaaagc taatataggt atatctacaa
    37561 ctggagtagc tggacctggt ggaggaacca aagagaagcc tgtgggactg gtttatactg
    37621 caatatatat aaatggaaaa acaatagtaa agaaaaatat atttaatggg gatagaagaa
    37681 agataagatt aagagctact agagatttgc ttaatgagtt gagaatacaa ttagaaaagc
    37741 tttaagttat tttgattaaa gtattagtaa aatataaaat tgtttttgga ggcttttaat
    37801 ttatgataaa acatatattt tgtgatttag atggaactct atatgaaaat ggaaccataa
    37861 caaaagaaga tatagtagca attgaagaaa tagaaaaaaa gggagtacag tttaatgttg
    37921 ctacaggtag aatttttaaa caagcacata atattatcaa agatagttta gatatgaatg
    37981 gatactatgt atgtgaaaat ggttcattta tatatgataa agattataat gtaattttta
    38041 aaagaacgat agatgataat ttagtaaaaa aagtcattga tagatttgag tcaagtgatg
    38101 cacaacttta ttttaaatat aagggtgatg taattgtggg tagtgatacc actgctttta
    38161 gacattattc tagtgacttt atagttgacc cagactttga aaaaagaagt agttttgata
    38221 atcttatagg aaatataggc gtatgttctg aaaatttgga agagttatcc agaatagaac
    38281 tttatctaaa aagtgagttt agtgaagtct tagaaatata tttttctgga acatatactt
    38341 taaatatagt tcctaaaagt gtgtcaaaaa gaggaagtat agagcatgta ataaaaacgt
    38401 taaatgtaag tccagatgaa gttgctacta ttggggattc tccaaatgat atttgcatgt
    38461 tagaaggatt taaatatagt tttgcaatgt ctaaggcaag ggaagatgta aagcaaagtg
    38521 caaattatgt agtagatagt gtaaaaagtg ccatagatgt tataatggag ataaatagtt
    38581 agtataaaaa atgactgtcc tagagaagta taaaattata ttctatagag cagtcatttt
    38641 aattatattc aattataagt ttatataagt ttaaaaattc ttaagttaac cgcaatttgc
    38701 tatatgatat gattttattg tattagataa aatagagtca agtatataat ttatgacact
    38761 attacaagta taagctgaaa attttaaatc attcatataa tcagttttct ttttgtaatc
    38821 gctataaagt ttagtaaaaa acttctcaaa actactattt atagtaccac atttaatttt
    38881 agataaatca acatcttttg caaatgctcc aagttctctt tcataagtaa catgtttatt
    38941 taagctatgc ttaaactccc aacgttcact gtgagctaca cagaaaaaat cacataaaaa
    39001 atgacatatg actcctagtt cctgactaag tctacttact gaaaagtatt tagaaagact
    39061 atctatatta agactgcata gatagtttat tttagaaaca atcatattaa atgactcatc
    39121 taaataatgt ttttttaatt tatatttaga aaaagcatca ggctttatat tgccatatat
    39181 aaaatttttt tcgcttataa agaatgtttt attttcatct atattatcaa gtattgattt
    39241 tgccataata aaatgggtat tcattaacat atttacacct cgtatagttt attagactta
    39301 ttaaatatat tattatgata tgatatttat gataaaagtt taatggtaaa tatatgaaac
    39361 taagtcaaca gtatttattt aaataatttc aaccatattc ataataacag taaatggcca
    39421 aatttacaat atgtaaatag tgttaagttt ttatttatca aatttaaaat taagtattag
    39481 tatactttct aattaaccta ttggctttag actgtgagat ttcaaggtgt ttggctactt
    39541 tataagaact accaaatttt tcataagcat ccaaaattat atttttttca tactctttaa
    39601 ttaaagtgtc aaaattttta tttttataat tagtagattt tgagtcagat ataataacct
    39661 tattaatttg ggatatatgt gttttatttc cattattggt aatatgtata ttttttgtaa
    39721 gaatgtttgg taagtcatct attgttatat gattatctgt acaagtaagc aagatttttt
    39781 gaattatatt ttgtaattct cttatattgc ctgggaatct ataatttgca atagcatcca
    39841 tagcttcttt agaaatagtt ttatttagat tgaagtcact tgagtaccta ttaaaataat
    39901 attttataat ttccactaga ttatcccttc tttctctaag aggaggcaat tttatttcta
    39961 taacatttaa tctatagtat aaatcttctc taaacttttt ttcttttact tttgaaacta
    40021 aatcaacatt tgtagcagat ataattctta tatcgacctt tttatttttt aagctcccaa
    40081 ctggagtaaa agttctatcc tgtattaatt gcaaaaattt tgcctgtaga ttttgtggta
    40141 tatctcctat ttcatctaaa aaaagagttc ctccattagc taattctacg agcccaactt
    40201 tacctttagt acttgcacct gtaaaagcac ctgaagtata tccaaaaagt tcagattcaa
    40261 gaagttgtgg agatatagtt gcacaattta ttgttataaa tgggccatta gatctattgc
    40321 tgttgtggtg tatatatcta gcaagtgagc tttttcctgt tccagattca cccaataaaa
    40381 gtacacttac atccgtttta gaaacatgat ttactgtgtt tatgattggt ctcataacta
    40441 ttccgtccat aattacagta tcctcaattc taagtgcagt tctgtatagt gtttctactt
    40501 cagatttata tttttttact tgcttttttg tgtcttcaag tttatttttt atattattaa
    40561 gttcagttat atctctacaa ttttcaacag taaattctaa gtttccattt tcatcaaata
    40621 taggggtagc agttatgata agctttttcc ctgtttgagt atcttgttcc agtgaaaatt
    40681 tagtctttgt ttttaataca attggtatgg ggctttttgt tgaatatcca gagtcttcta
    40741 aaaaagaaac attttttcct aaaatatctt ctttttttag accataattt tcttcaaatg
    40801 ctttattaca atatattatt attcctttat catcagaaac acatatttca tcatgggaag
    40861 cttctaatat tttttgataa aattccaaat ctaaattcat ttttatattt tcctcctaaa
    40921 tttttaatga tatattaaaa taggtttaat tgattaatat acttaaaatt tgata
//
LOCUS       DAMJVI010000022        40852 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.022, whole genome shotgun sequence.
ACCESSION   DAMJVI010000022 DAMJVI010000000
VERSION     DAMJVI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40852
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.022"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            203..862
                     /gene="cprR"
                     /locus_tag="PC545_001281"
     CDS             203..862
                     /gene="cprR"
                     /locus_tag="PC545_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HDF2456058.1"
                     /translation="MSKILVVDDELDMLKLIENVLKRDGHEVKVISDVEKAMNMDFNY
                     FNLIILDVMMPKMDGFEVCKTIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVSIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHIKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            1073..2131
                     /locus_tag="PC545_001282"
     CDS             1073..2131
                     /locus_tag="PC545_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2456059.1"
                     /translation="MDRLKNIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPKKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            2382..4526
                     /locus_tag="PC545_001283"
     CDS             2382..4526
                     /locus_tag="PC545_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2796..2798,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HDF2456060.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYTASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPNLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIETQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAEKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYQSLKDKMTATNI"
     gene            4575..5408
                     /gene="fdhD"
                     /locus_tag="PC545_001284"
     CDS             4575..5408
                     /gene="fdhD"
                     /locus_tag="PC545_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDF2456061.1"
                     /translation="MNNIYSKTKLQENSKDKVFENSYSLVDYKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNDKYINTFLCTPYNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIQEKQKNQDEQIIFLNSLDYITCAPVKSNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSVFNFDKKIVTCEDVARHNAMDKAIGFCVLNDITLKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            5419..5592
                     /locus_tag="PC545_001285"
     CDS             5419..5592
                     /locus_tag="PC545_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456062.1"
                     /translation="MCTKKEEQRYSKCEICGVLIDDTDKVYNNTYNLCKKCKRNISTE
                     KIVKYINIYDESK"
     gene            5650..6201
                     /locus_tag="PC545_001286"
     CDS             5650..6201
                     /locus_tag="PC545_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2456063.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRQCEDAPCLNSCPQKAIVKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYEDGKEVEQVILDENKKIAYKCDLCKDNEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            6226..7602
                     /locus_tag="PC545_001287"
     CDS             6226..7602
                     /locus_tag="PC545_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2456064.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            7602..8117
                     /locus_tag="PC545_001288"
     CDS             7602..8117
                     /locus_tag="PC545_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2456065.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNLDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(8195..9478)
                     /locus_tag="PC545_001289"
     CDS             complement(8195..9478)
                     /locus_tag="PC545_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugated bile salt MFS transporter"
                     /protein_id="HDF2456066.1"
                     /translation="MNTLLNNKFSIKNFSNAWVIVLGCMLIQAVPFSIVASIQPQFIS
                     YVVEGEGFSLASFSLIFTIGTIASAIASPFIGVLFSKVNIKILYLLGCIISSIAFATF
                     SICTELWQFYTVSCIVQIGATIISAIGIPVLISAWFDEESKGKALGFAFAGGSIGSIF
                     LQPLAVRLLATQGYSRSYLIFGIIALIVGIPVILFILRMPKDINEKVKSKGKKEKASD
                     TISPSVWGYTLKEAKKIKYFWIMTIGFIFVGIYVSSLSVQYPTYLRNYLNPILIGTVG
                     STFALFALIGNLFGGLLFDKFGQTKPLIISFVLVLIATLSLLLVDKSSTFAFVFAILK
                     GLSVFAYMIGPAYLTGSFFGQKEFGSIYGIVQLIFAVGFALGSTLFGLLVDNFGYRFS
                     WSIIILSVIIAYTLLIITSIGMKKLNKERLDKLMQ"
     gene            9666..10088
                     /locus_tag="PC545_001290"
     CDS             9666..10088
                     /locus_tag="PC545_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HDF2456067.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGENIIRDILDGNISNECENGIKSKSVIFNNTPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            10190..11128
                     /locus_tag="PC545_001291"
     CDS             10190..11128
                     /locus_tag="PC545_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDF2456068.1"
                     /translation="MKVLFTINYGKEKFDKIRELGYDVIHYSEDSIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGVE
                     IWGVNTDGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECVEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYIMENKPVDNVVDISRGY"
     gene            11314..13338
                     /gene="ligA"
                     /locus_tag="PC545_001292"
     CDS             11314..13338
                     /gene="ligA"
                     /locus_tag="PC545_001292"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDF2456069.1"
                     /translation="MSVKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEEYDLESHSESLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLDYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVDLERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDEVISATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLTKANDLGIKVIDEERFLELLKLSSKDEVKAVLES"
     gene            13498..14847
                     /gene="rph"
                     /locus_tag="PC545_001293"
     CDS             13498..14847
                     /gene="rph"
                     /locus_tag="PC545_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDF2456070.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKIIKEMPITNFV
                     SAISVGIVNGIHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRADLNLL
                     LELGEKGNKELIRLQREALGEISDEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIEIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNIPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            14847..15317
                     /locus_tag="PC545_001294"
     CDS             14847..15317
                     /locus_tag="PC545_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2456071.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEVDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDDIKIEEIRI"
     gene            15511..16797
                     /locus_tag="PC545_001295"
     CDS             15511..16797
                     /locus_tag="PC545_001295"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDF2456072.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            16947..17531
                     /gene="clpP"
                     /locus_tag="PC545_001296"
     CDS             16947..17531
                     /gene="clpP"
                     /locus_tag="PC545_001296"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2456073.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            17550..18800
                     /gene="clpX"
                     /locus_tag="PC545_001297"
     CDS             17550..18800
                     /gene="clpX"
                     /locus_tag="PC545_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDF2456074.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(18871..19398)
                     /locus_tag="PC545_001298"
     CDS             complement(18871..19398)
                     /locus_tag="PC545_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2456075.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATIGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(19386..19898)
                     /locus_tag="PC545_001299"
     CDS             complement(19386..19898)
                     /locus_tag="PC545_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HDF2456076.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            20078..22441
                     /gene="lon"
                     /locus_tag="PC545_001300"
     CDS             20078..22441
                     /gene="lon"
                     /locus_tag="PC545_001300"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDF2456077.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGRVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLSNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVINKSDLETYLGKDMFKYQLAEVNPQIGLVNGLAWTEVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPEFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            22431..23042
                     /locus_tag="PC545_001301"
     CDS             22431..23042
                     /locus_tag="PC545_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDF2456078.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(23308..24678)
                     /locus_tag="PC545_001302"
     CDS             complement(23308..24678)
                     /locus_tag="PC545_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456079.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPANEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQNKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGITALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGYVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATIRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNQSYWIAASVCFVGLILTVFFMRK
                     TDKSIS"
     regulatory      complement(24853..24968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            25256..26335
                     /locus_tag="PC545_001303"
     CDS             25256..26335
                     /locus_tag="PC545_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456080.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNKQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVKKVY
                     YLDGEIGTGKTTLLNKVYKKAIEKGLNVEVYHYPLIPEKIETVLLTDLDIGITISTTF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDKLIKRILKYEENL"
     gene            26727..27407
                     /locus_tag="PC545_001304"
     CDS             26727..27407
                     /locus_tag="PC545_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HDF2456081.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKDKLDKEINSMKQNQKNSEEISEIETLRDSLSYSDVKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKVNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            27400..28779
                     /locus_tag="PC545_001305"
     CDS             27400..28779
                     /locus_tag="PC545_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HDF2456082.1"
                     /translation="MIKIILEESSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVIKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDNFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            28950..30035
                     /locus_tag="PC545_001306"
     CDS             28950..30035
                     /locus_tag="PC545_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDF2456083.1"
                     /translation="MRLSKNIFYNLEKFYLVLNRISDKEIRVICKEIGILLESGCEIT
                     KIFEIIESQSSKKAKNLLSIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKMKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTNNG
                     IEPPLITRVLMGISTVVTNNLAYIIFSFILFSIGSIYFFITNDNIKKLINSLKFKIPF
                     IKKINQLVATTRFSRALSVLISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANVFPKLFISMANTGEESGKLDKSLNVINKFYENELNNKIEQIMKFIEPM
                     LIVFMGLIIGTFILAMVVPMFDAITNF"
     gene            30089..30448
                     /locus_tag="PC545_001307"
     CDS             30089..30448
                     /locus_tag="PC545_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDF2456084.1"
                     /translation="MINLINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKL
                     NADYTNAANIVTAASMAINDDEKTIDSLSVETLKEKGYLNTVPVPQSTSGKFELVIND
                     SGTDISVNINSKQFYPK"
     gene            30501..31373
                     /locus_tag="PC545_001308"
     CDS             30501..31373
                     /locus_tag="PC545_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HDF2456085.1"
                     /translation="MAIIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFDIK
                     NDSNSIEKIKYIEDVVRFVKEEIGKSKILYKNLYFNIQIQDIVIRNVEVLNTKKKRDI
                     LSMIEFEITQYIPININNYSIKYKVINSKNDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIRETYLLSKEGK
                     SYEFIKRLLRDFKYVYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDADLE
                     DNRINYISNIGMII"
     gene            31374..31817
                     /locus_tag="PC545_001309"
     CDS             31374..31817
                     /locus_tag="PC545_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456086.1"
                     /translation="MKVDKTINFLNGFKKTKPCNKIYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSRHTLPTTDKTREKFSKIKLNEIKKIYETIGINIITKISIDK
                     NKLEIEGVCSDLDVLNNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            31832..32416
                     /locus_tag="PC545_001310"
     CDS             31832..32416
                     /locus_tag="PC545_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456087.1"
                     /translation="MRLNNREKNLLKILLILVIILASDIAVSYLEENLTSLKNEKQNI
                     INKINNQDNYQIKDNKHIDDKYIESVESNKNEELKNNFNNSFLDKKTNKDIVLLVENE
                     LRDIVNVEYINQESSLDNHNKKQVNLEIKVNGELANLLKLEGVMKKLRLNNKLEKIEI
                     NKLKVNNFDVQNIPNEYKQEKDNIVECKMTVRVI"
     gene            32669..33706
                     /locus_tag="PC545_001311"
     CDS             32669..33706
                     /locus_tag="PC545_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HDF2456088.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSMFESYVSIYEVKEVSAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKRERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            33787..34152
                     /locus_tag="PC545_001312"
     CDS             33787..34152
                     /locus_tag="PC545_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456089.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            34157..34474
                     /locus_tag="PC545_001313"
     CDS             34157..34474
                     /locus_tag="PC545_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456090.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGSEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            34522..36126
                     /locus_tag="PC545_001314"
     CDS             34522..36126
                     /locus_tag="PC545_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456091.1"
                     /translation="MLRVSNVKLGIDKDISLLKDVILKKLKIKEKNLVKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILNSKIKDVTKIKEIRYVNVPVGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVIINDTEKIDTDTIILAVGHSARDTYEMLYSRGIKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASASNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGSVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSIKNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            36250..37878
                     /locus_tag="PC545_001315"
     CDS             36250..37878
                     /locus_tag="PC545_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2456092.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPVALGVGIILYLFGNKPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPLLGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPVVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTSLIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAALDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNIYFSEEGQSELD
                     VMYNRVVSAYTYALESMRSDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            38143..39492
                     /locus_tag="PC545_001316"
     CDS             38143..39492
                     /locus_tag="PC545_001316"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDF2456093.1"
                     /translation="MGKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSADKIKSDSDVLLVIGIGGSYLGSRAAIDMVSHSFRNG
                     LKKEQRKAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDASKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKSNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITISSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            39648..40727
                     /locus_tag="PC545_001317"
     CDS             39648..40727
                     /locus_tag="PC545_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2456094.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVSKSTGSSTP
                     IVVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTSNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTNMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASQAEGLGFAIPINTAKSIVEEVIKNGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TFSKGASTKITVNRGGKAVTINVNF"
BASE COUNT        16782 a         4309 c         7356 g        12405 t
ORIGIN      
        1 ttttatattt aagtaaatac gtgtaaaatg aatataaagt ttttgaaata taaagttttt
       61 gtatctaaaa tattttattt gtaaaatagt aattaattat tgtaagctat aatatgagag
      121 tattatggtt aatgtgaatt ttaaagatat tattaaatat ataaattaga atactattaa
      181 acggataaat ggaggagtta ttatgtctaa gatattagta gtagatgatg agttagatat
      241 gttaaagttg attgaaaatg tgttgaaaag agatggacat gaagtaaagg taattagtga
      301 tgtagaaaaa gctatgaaca tggattttaa ttattttaat ctaataatat tagatgttat
      361 gatgcctaag atggatggtt ttgaagtatg taaaactata agaaataagg ttgattgtcc
      421 aatactgttt ttgacagcaa aaaatatgga aagtgatatt atgtacggtc ttggtatagg
      481 tgcagatgac tatataacca aaccttttgg tgtaggagaa ttaagggcta ggataactgc
      541 tcatttaagg cgagaaagta gagaaaaaaa gaatttatta actatctcaa atgttcaatt
      601 taacttttta ggcaaagaag tttcaattga tggtaaaaaa attaatttta ctaaaagtga
      661 atatctaata tgtgaatttc tagcaaaaaa taagggacaa gtattttcaa aagaaagaat
      721 atatgagtcg gtatatggtt ttgatggaga aagtgatata agtactatca cagagcatat
      781 aaaaaatata cgaagcaaat taaaacaata tgatatttat acaatagaaa ctgtatgggg
      841 gatagggtat aaatgggtgt aataagtata agtgttgttt agtgaaaata ttatatatat
      901 ctacaaaatg tttgtaaaat attaatctat atattaaaat ttaatttata gattaatatt
      961 ttttgtaaat gacaaaattg taaggtattt tgttataaaa tattaagatt catagtgtat
     1021 actaataata tagtatttag tataaatctt gagcaaagga tggttatttc taatggatag
     1081 actaaagaat attagaaaga gtttactaca gtatacattt ttatttttgt taataggtat
     1141 aacaatttac ctagtgttta aaacattgga tattaaaatg ctatcaaatg ttatggttat
     1201 ggtagataag aagtttttat tcataggtgc tttggcaata atgtgtcaca taatgcttga
     1261 aggagtagta atgaagataa ttatagaaag cactcacaaa gttaatatta gatttattgg
     1321 ttttaagcta gccattatgg gattttatta taatttaata actccttttg cttcaggaag
     1381 ccaaccagta cagatatatg tacttaaaaa atgtaaaatg ccacttagta aggcaagtgc
     1441 agttgttacc aataagtcta ttatcttcca aatagtagtt actttttatt gtacaatact
     1501 tgttttaatg aattttgcta tgttagaaaa acaaatgaaa atgattatgc ctctagtgtt
     1561 tctagggata gccattaatt catttgtctt aattatgata atattagtta ttttaaatcc
     1621 taaaaaaata aaatatttta tgagaattac tataaagcat ttatcaaaaa ttaagttttt
     1681 aaaattttta gataataaga ttgaatccat agaaagtttt atagatgatt atagtaaagc
     1741 aataaatttt tttgcaaaga ataaaaaggt attaatttca actataatag taacttttat
     1801 tcaattaagt gcttacttta gtgtttcatt ttggatatat aaagcattta atttacaagg
     1861 ttatagctat atttatatac tgacattaca agcattttta tatatggcaa tatctccaat
     1921 accaacacct gggaatgtag gagctaatga attagccttt ttcactatat tcaaatcagt
     1981 attccctcaa cctttgatgg gatatgcagt atttttatat ggtggattta tgtactattt
     2041 gattctgatt ggaagtggaa tatttactgt aattactcac tatagaatga aaaatagaat
     2101 tggcagaaat attgttttaa gtgaaaacta aataagttta aagaatatat attgagtata
     2161 ttaaaaaata tgtcataaga actagaatat tttaattaaa tggttgaaat ataaagatta
     2221 ttatgacata ttttttttga aatttaagag gaattgtgag aaaattgttg aatttaatag
     2281 atagtattgc aaaatattta tgattttgca tattataaaa acactcatta tctttaagat
     2341 aaattttact tatcagggga taaatctaag gggggaattg tatggagaaa aaaattttaa
     2401 cagtttgtcc atactgtgga gctggatgcc aattatatct agtagtaaaa gataataaga
     2461 tattaagagc tgaaccggct aatggtagaa caaatcaaga aagtttgtgt ttaaaaggtc
     2521 gttatggatg ggattttcta aatgacccta aaattctaac acctaggcta aaaaagccaa
     2581 tgataagaaa acaaggtgta ctagaagagg ttgaatggga tgaggctata aactatacag
     2641 cttcaagact aaatgaaatt aaagaaaaat atggtcctga ttctattatg ggaacaggtt
     2701 ctgcaagagg acctggtaat gaagcaaact atataatgca aaaatttatg agagctacaa
     2761 taggaactaa taatgttgac cattgtgctc gtgtctgaca tgcaccatct gtagccggtc
     2821 tggcttactc attaggtagt ggtgcaatgt ctaattctat accagagata gaaaatgcag
     2881 atgttttatt tatatttgga tataatggag cagattcaca tccaatagtg gcaaatagaa
     2941 tagtaaaagc taaaaaaaat ggtgctaaat taattgttac tgaccctaga gtaactgaat
     3001 ctgcaagaat tgctgatata catctaccta taaaaggtgg aacaaacatg gttttagtaa
     3061 atgcttttgg aaatgtatta atagaagaag gtctttacaa taaagaattt gttcaaaatc
     3121 atacacaagg atttgatgag tataaagaaa ttgttaaacc atatacagct aaatatgctg
     3181 aaaagattac aggaatccca gaagagctta taagaaaagc tatgagagaa tatgcaaaag
     3241 gtaaaaaagc tatgatactt tatggaatgg gtgtctgtca atttggtcaa gctgttgatg
     3301 tagttaaggg acttgcatca attgctctac ttacaggtaa ttttggtaga gaaagcgttg
     3361 gaataggacc tgtacgtgga caaaataatg ttcaaggagc ttgtgatatg ggagctcttc
     3421 ctaatgtata tcctggctat caaaatgtaa ctgatgataa gataagagaa aagtttgaaa
     3481 aagcttgggg ggtaaaatta tctcctaata atggttatag tttaactcaa gttccaaatt
     3541 tagtactaaa agagaaaaag cttaaagctt attatatatt tggagaagac ccagtgcaaa
     3601 gtgacccaga tgcatcagaa gttagagaag cattagatga attggaattt gtcattgttc
     3661 aagatatatt tatgaataag acagcacttc acgctgatgt tatacttcca gctacttctt
     3721 ggggagaaca tgagggagta tatacatgtg cagatagagg attccaattg atgagaaaag
     3781 ctatagagcc acaaggagat gtaaaaccag actggcaaat tataagcgaa atttctacag
     3841 caatgggata tcctatgaat tataaaaata ctaaggaaat atgggacgaa cttagacaat
     3901 tatgtccaag tttcttaggt gctacttatg aaaagataga aactcaagga tgcgttcaat
     3961 ggccttgtaa gagtgaaagt atggaagata aaggaactat gtatctgtat gaaggtcaaa
     4021 agttttctac tcctaatggt aaaggtaatt tgttcgctgc tgaatggaga ccccctatgg
     4081 aagttgaaga tgatgaatac ccatttagtt tatgtactgt aagagaagtt ggacactatt
     4141 cagtaagaac aatgactggt aattgtagaa cacttagttc attagaagat gaaccaggac
     4201 gtgttcaaat aaattctaat gatgctgaaa aattaggaat tgaagatgat gaattagtta
     4261 gaattagttc aagaagagga agtgtaataa caagagcaac agttactgat agagttaagg
     4321 aaggtgcaac atatatgact tatcagtggt gggttggagc ttgcaatgaa cttacaatag
     4381 caaatttaga tcctatatct aaaacaccag agtataaata ttgtgctgtt aaacttgaaa
     4441 aacttgaaga ccaagaatta gcagaaaaat gtgtaagaga agagtatcaa agtttgaaag
     4501 acaaaatgac agctacaaat atctaatgat ttattagatt taagatttat aaaaggcaga
     4561 aaggctaata ttttatgaac aatatatact caaaaactaa attacaagaa aatagtaagg
     4621 ataaagtttt tgaaaatagt tatagtctag tggactacaa atatttaaat tcttttaatg
     4681 tagaagtaag caaaataaat gaacatgaag tatcaataga taatgaagaa ataattgcag
     4741 aatatccact tagttttatt ttaaatgata aatatataaa tacattttta tgtaccccat
     4801 ataatttaga ggaacttata gtaggattct tgaaaacaaa aggatacata gaaaacaaaa
     4861 aaagcttatt aaaatttgag ataaatgaga gtgaacgttt tgcaaaagta actattcaag
     4921 agaaacaaaa aaatcaagat gagcaaatta tatttttaaa ttcactagac tatataacat
     4981 gtgctcctgt aaagagcaat ataaaaataa attatgatac gatttacagt ataatggata
     5041 gaaatttaaa ttcatcagaa ttatttaaaa atactggtgg ggtacatagc gtatctgtat
     5101 ttaattttga taaaaaaata gtcacttgtg aagatgtagc aaggcataat gcaatggata
     5161 aggctattgg attttgtgtt ttaaatgaca taacattaaa agataaaatt gtggctgtaa
     5221 gtggaagaat atcattggaa atgatattaa aagcagctat gactcaaatt cctattgtag
     5281 tatcaaaatc agcaccgact aatttatcaa tagagctttc taaaaaatta aatataacgc
     5341 ttattggatt tgtaagagga agacggatga atatttatgc aaatccacag agagttatca
     5401 aaaagtgaga atgaatatat gtgtactaaa aaagaagagc aaagatattc aaagtgtgaa
     5461 atctgtggag tattaattga tgatactgat aaggtgtata ataacacata caatttgtgc
     5521 aaaaaatgta aacgaaatat aagtactgag aaaatagtga aatatataaa tatatatgat
     5581 gaaagtaagt agacatatga tgaaaataac tagagggttg gaaatataca aattgaaagg
     5641 tgggataata tgaaaaataa aataggctct tttgtaatag cagacccaaa caaatgtaca
     5701 ggttgtagag cttgtgaagt agcttgtttt acaatgcata atcagcataa taatgtgggt
     5761 tatacagttg gaacagttga tatacctgtt atacctagac tttacttagt aaaaggtgac
     5821 aatttctgta tgccaataca gtgtagacag tgtgaagatg ctccttgttt aaactcatgt
     5881 ccacaaaaag ctatagttaa agaaaacaat attatgtctg taaatgaaga aaagtgtata
     5941 ggatgtaaaa cttgtctgct agcatgtcct tttggagcta tagacttact tccacaatat
     6001 gaagatggta aagaagtaga acaagtaatt ttagatgaaa ataagaagat agcttataaa
     6061 tgtgacttat gtaaagacaa tgaaaaaata gcttgtataa gtgcatgtcc tcaggaagca
     6121 ttaaaacttg ttactccaat agatgacaaa aaagctaaga atagacaagc tgcacttagt
     6181 ttattgttaa caaataaata aaggatacag gggtaggtga cgtttatgat aataaatata
     6241 gataaggatt tatgtactgg atgtagagaa tgttcaaagg tctgtccagt aaatgctatt
     6301 gaaggtgaag agggaaagcc acaagagata aatttagata gatgtgttat gtgcggtcaa
     6361 tgtgttcaaa cttgtaaatc ttatgcttca gttatagatg aaggatttga gtttttacaa
     6421 gagaaaaaac aagaaagaga gataccagag tctataaatg aacctatatt tgcagcatac
     6481 aatgtatgtg atatagataa ggtgaagaaa gctttaagtg acccaaatgt attcactatg
     6541 gtccaatgtg caccagcagt tagagttgct ttaggagaag atttcggata ttatttagga
     6601 tacttaggtg ctgggaaaat ggcagcagca cttagagctt taaattttga tagagtctac
     6661 gatactaatt ttgcagcaga tttaactata atggaagaag gtagtgaatt aattaaaaga
     6721 gtgacagaag gtggaactct tccaatgttt acatcttgtt gtccagcttg ggtaaaattc
     6781 atggaaaaaa attatcctaa attaactaat catttatctt cttgtaaatc acctcagcag
     6841 atgggtggtg caatatttaa aacttatggt gcagagataa ataatgtaga tgcatcaaag
     6901 atatttagtg tttctataat gccatgtaca tgtaaaaaat atgaatgtga tagagaagaa
     6961 atggattcta gtggatatag agatgtagat gtagtattaa ctactagaga actagcttat
     7021 ttaataaaag acatgggaat tgattttaaa aatttaagag aagaaaaatt tgatagtcct
     7081 ttaggtagtt atagtggagc tggaactatc tttggtgtta ctggtggggt tatggaagct
     7141 gctataagaa caggatatga gcttataact ggagaaagta ttcctgatgt agaagtaaaa
     7201 caagtaagag gagaaaatgg ttttagaaag tcaactatta aaataggaga cttagactta
     7261 agagttggag tagtaagtgg attaaaaaat gtaataccag tgcttgaaga cttggaaaaa
     7321 ggaaaattag atgtagattt tattgaagtt atgacttgtc ctgttgggtg tgtaagtggt
     7381 ggaggtcaac caaaaatttt actagaagaa tataaggaat tggcttatga aaatagaaca
     7441 aaagcaacat acgtgcatga tgaacattta gcaattagaa aatctcatga gaatcctgat
     7501 attaaaaaac tgtatgatga atatctggta gaaccattag gagaaaaatc tcatcatcta
     7561 ttacacacta catattgtat aggaaaggaa gtggctaagt gatgaactat tttgttatag
     7621 cagaccctga taaatgcata ggatgtagaa cttgtatgat aggatgtgta gttgcacatt
     7681 cagatgaaga tattttttac caaaatctag atgaaataaa ttttaatcca aaactttctg
     7741 tcataaagac aaaagaagta agtgctccaa tacagtgtag acattgtgaa gatgctcctt
     7801 gtgcaaaggc atgtccaaat ggtggtatag ttagggttgg gaacacaata aaaataaatg
     7861 aagaaaattg tataggatgt aagacttgta tgttagcatg tcctataggt gcaatagaca
     7921 tagttacatt aaaagatgta gatgaaggaa agctttgctt tagagaaaga atgacagcaa
     7981 ataagtgtga tttttgtata gaatcgccag aagggcctgc atgtgtaaat gtttgtccaa
     8041 ctaaggcatt tactatagtt aaggatgagg atatagatag taaagttaaa aataagagaa
     8101 aattagctat attgtaatat aaaaaagatg gagttttatt tatgattaaa agctccatct
     8161 tttttattat aatatttata tatctaaaaa tcaattattg cattagttta tctaatcttt
     8221 ctttattcaa ttttttcatt cctatagaag ttataataag taaagtatag gcaattataa
     8281 ctgataaaat tataatagac caagaaaatc tgtatccaaa attatcaact aacaatccaa
     8341 ataatgttga acctaatgca aatcccactg caaaaattaa ttgtacgatt ccatatatgc
     8401 taccaaactc cttttgacca aagaaagagc cagttaaata agcaggacca atcatataag
     8461 caaatacaga aagacctttc aaaatggcaa atacaaatgc aaaagtagaa gatttatcta
     8521 ccaatagtaa tgataaagtt gctataagaa ccaatacaaa agatataata agtggctttg
     8581 tttgaccgaa tttatcaaac aataatcctc caaataaatt accaatgaga gcaaataaag
     8641 caaatgtgga gcctactgtt cctattagaa taggatttag ataatttcta agataagttg
     8701 gatattgtac ggataatgat gaaacatata taccaacaaa tataaatcca atagtcataa
     8761 tccaaaaata tttaattttt ttagcttctt ttaatgtata accccatact gaaggagaaa
     8821 ttgtatcaga tgctttctct tttttacctt tagattttac cttttcatta atatctttag
     8881 gcatcctgag tatgaataat attacaggta taccaactat taatgctata attccaaata
     8941 ttagataaga acgactgtat ccttgagttg ctaataagcg aactgcaagt ggttgtagaa
     9001 atatactacc aatagaacca ccagcaaaag caaagcccaa agcttttcct ttgctttctt
     9061 catcaaacca agcacttatt aaaacaggta ttcctatagc agatataatt gtagcgccaa
     9121 tttgaacaat acatgataca gtgtaaaatt gccatagttc agtacatata gaaaaagtag
     9181 caaaagcaat actagaaatt atacaaccta ataaatatag tatcttaata ttgactttag
     9241 aaaataatac acctataaaa ggtgatgcaa ttgctgaggc aatagtacca atagtgaata
     9301 ttagagaaaa agaagctaaa ctaaagcctt ctccttctac aacataactt ataaattgag
     9361 gctgtatact tgctacaata ctaaatggaa cagcttgtat caacatacaa ccaagtacta
     9421 taacccaagc atttgaaaag tttttaatgc taaatttatt gtttagtaat gtattcatgg
     9481 tatctctcct ttaggttatg atttgtagat attaatctaa ttattctaat tttatatatt
     9541 agttttggat agcccaacta tttaaatata atttgggtat taatataaaa attaactata
     9601 aaaattgatt attttaaatg tatggggtaa attaaagtat ataaataaat gaaaggatgg
     9661 ataaaatgtc atttgaaaaa ataaataata ataacctaga taaaatacac aacaggaagt
     9721 gtattatgct tgtaaacttt aataaaaaag aattatcatt aattaaaaac gtaagtaact
     9781 ttgctggaat aagagaccat atcatcttaa gtgataaaaa tggagagaat ataataagag
     9841 acattttaga tggaaatata tcaaatgaat gtgaaaatgg gattaagagt aagtctgtaa
     9901 tatttaataa cactccaagc acacaagtaa atgcattttt agaagggcta aaaaaagtga
     9961 gaataaatag accttttaca gctatggtaa cagagacatc tattgattgg acattgaata
    10021 atttaataga aaatttagtt caagaacgta atgctatgtc tttaggaaaa acaattgaac
    10081 ataagtaaat ttaagatatc ttatagtaat tttaactaat gtacattgag tatcatatta
    10141 tattttagaa tataaaattt taaatgtggg tagatggagg agatttgaga tgaaagtttt
    10201 atttacaata aattatggaa aagaaaaatt tgataaaata agagaattag gctatgatgt
    10261 catacattac tctgaagata gtattgaaaa taatgaggat gtaaatacag cagatgtact
    10321 agttacatac aatccatttg agagattaga tatttctaag atgaaaaatt tacaatatat
    10381 acagacaact agtgtaggta tagaccaact tccaaaagaa gaggtattga agagaaatat
    10441 taagatagct aataatagag gcggatatag tgtacctatt ggtgaaagta ttgtattgta
    10501 tattttagaa atatataaaa atagtgcaaa attttttaaa caacaacaaa ataaagaatg
    10561 gaagatggat ttttctgtat cagaattgta tggacaaaga gttggatttt taggaacagg
    10621 tactatatct tcagaagcag caaaaagatt aaaagctttt ggtgtagaaa tatggggtgt
    10681 caatactgat ggaagtagta aaaaatactt tgataagtgt tttgctactg aagatatgaa
    10741 tattgtattt gaagaatgtg atatagtagt tgtagcaatg ccttctacaa aaagtacaga
    10801 tggaatagta aacaaagata tgtttaaact tatgaaagat ggttctgtat ttataaatgt
    10861 aggtagagga aatacagtta atcagaaaga tttagaagaa tgtgtagaga aatttagagg
    10921 ggtagcatta gacgtatttg aaagtgaacc tttaaacaag gacaataagt tgtgggagtg
    10981 cgaaaatgtc atagtaactc ctcataattc ttgggtgtca gataaaaaca aagagagaac
    11041 atttaatatg gtttataata atttgaaaca ctatataatg gagaataaac ctgtagataa
    11101 tgttgtagac atatcaagag gctattaaaa ataaaaaatt ttgataaaaa taaaaactct
    11161 tattagtcgt attaaagata ctaataagag ttttttttgt aataaattta aagcccaaaa
    11221 agtgatatac taaaaaatat ataaatagga gataattaat tatagggaaa tttcaaaata
    11281 atttaatttt aaaaatatga agaggtgcaa aaagtgagcg taaaagatga aattaataaa
    11341 cttatagaga caataaacta ccataatgaa aggtattata atcaagatag cccagaaata
    11401 tcagattatg aatacgataa gttaatgaag gagttaattt ctttagaaga agaacatcca
    11461 gaactaaaga gaattgattc tccaacaaat agggtaggag gtaagccatt agataagttc
    11521 aatcaaattg tacataaaac tcctatgttg agtttatcaa atgctttttc agagcaagat
    11581 ttgagagatt ttgataagag agttcaagaa tatgtgggag aaaatgttga atatgttgtt
    11641 gaatttaaaa tagatggtct ttctgtaggt ctaacttata acaatggaga gtttgaaaaa
    11701 ggagcaacta gaggagatgg agttgtagga gaagatattt ctcaaaactt aatgacagta
    11761 aaaagcatac ctcttaagat aaatgataaa aaagaactaa tagttagagg agaagtatat
    11821 atatcaaaag ataattttag aaaagtaaat gaacaacaag aagaacaaga acagccatta
    11881 tttgctaatc caaggaatct tgcagctggt tcattgagac aattagaccc aaaactaaca
    11941 gcaaagagac cattagatat atttgtattt aatatggaaa atatagaaga atacgattta
    12001 gaaagtcaca gtgaatcact agagtactta gaaaaactag gattttcagt aagtcagagt
    12061 tataaagttt gtaaaagtat agatgaagtt attgagcata ttgaatactg gacagataac
    12121 aggggtaatt tagactacga aatagatggt atggtaataa aagtaaacaa tataaaacaa
    12181 agagacgaaa tgggatatac agccaaaagt cctagatggg caattgcata taaattccca
    12241 gcagaaaaga agaaaactaa aatagtagat ataatagtag aagttgggag aactggtaca
    12301 ataacacctt cagcaattct tgaaccagtc agacttgcag gtactactgt aagtagagct
    12361 acacttcaca atgaagatta tataagagaa aaagatataa aaataaatga tacagtatta
    12421 gttcaaaaag caggggacat aatacctcaa gttttagaag ttataaaaga agaacgtact
    12481 ggagatgaaa tagattttaa aatgccagaa aagtgtccag tttgttcaga gccaacaata
    12541 agattagaag gagaagctgc tgtaaaatgt atcaatatgt cttgcccagc ccaaataaga
    12601 aggggaatta tacactttgt gtcaagggat gcaatgaata tagatggatt gggagaatct
    12661 ataattacac tgctattaaa tgaaaaaatt attcaagatg tatctgattt atattatata
    12721 aaaaaggaag atgtagtcga cctagagaga atgggcgaaa aatctgctga caatttaata
    12781 aattcaattg aaaaatcaaa agaaaatgat ttatggagac ttataaatgg attaggtatt
    12841 aaatttatag gagttaaagc agctaagata cttgcatata actttaagga tttagatgaa
    12901 gtaatttctg caactagtga gcaactagaa gaattagagg aatttggaag tataatggca
    12961 aacagcgttg tcgagttttt taaggaagac aaaaatatga acgttataaa caagttaaaa
    13021 aatgtaggag taaatacaaa atcattggat aatacaggtg aaaaagtaga aaaaatattt
    13081 gaagggatga aaatcgtatt gactggtaca cttcctacat taaaaagaaa tgatgcaaaa
    13141 gatatgatag aaaaaagagg tggaaaagct acatctagtg ttagtaaatc gacaacattt
    13201 gttttggcag gtgaagaagc tggttctaaa cttactaaag caaatgattt aggaattaaa
    13261 gttatagatg aagagagatt tttagaatta ttaaagttat catcgaaaga tgaagtaaaa
    13321 gctgtattag agagttaaaa agtcgcttaa tttagtagaa aactactata attaatgata
    13381 gaatagttca gtaagctaat aagattacga aaaatgacaa taattaatat ttgacaaaat
    13441 taaaaattat aaaaataaat ttataaaaaa taaaaaatat gagaaaggat ataaaaaatg
    13501 accagaagtt atgatagaag taacgaacag ataagacctg taaagataac tagagattat
    13561 acaaagtatg cagaaggttc tgttcttata gaaatgggag agacaaaggt tatatgcact
    13621 gcatcagtgg aagaaaaagt tcctcctttt ttaagaaata gtggtactgg atggataaat
    13681 gctgaatatt ctatgctacc aagagcaaca caacaaagaa agataagaga tgcatctagg
    13741 ggaaaaattg atggaagaac acaagaaata cagaggttaa taggaagagc aataaggtct
    13801 gttgtagact tgaataaatt aggagaaaga actatttggg tagattgtga tgtgattcag
    13861 gcagatggtg gaactagaac tgcatctata acaggggcat ttgtagcagt tgcagaagca
    13921 atatataaat tatataaagc aaagataata aaagagatgc caataacaaa ttttgtgtct
    13981 gcaattagtg ttggtattgt aaatggaata cacatgctag atttatgtta tgaagaagat
    14041 tctaaagcac aggtagatat gaatataata atgactgata agtgtgagtt tgtagaagta
    14101 caggggacag gagaagagaa acctttttcg agagcagatt taaatttatt gttagagtta
    14161 ggtgaaaaag ggaataaaga attaataagg cttcaaagag aagctttagg tgaaatatca
    14221 gatgagatat tgggtatgga ttatggtaat gaagtagtga ttgctactaa taatgctcat
    14281 aagctagaag aaataggtga aatcttaaaa gattttgaat ataaagtgta ctctttaaaa
    14341 gatgttgatt tagcaggtat agagatagta gaagatggaa aaacatttga gcataatgcg
    14401 ttgataaaag ctagaacaat agcaaaaaag actaaactta tagcaatatc agatgattct
    14461 ggcttagaag tagatgcttt aggcaaaaaa cctggtgttt attcagctag atatgcagga
    14521 gaacatgcaa cagatgaaga aaatagaaaa aaactgttaa aagctatgca aaatatacca
    14581 atgagcaaaa gaaatgcaag attcgtctct gcaatagcag tagtttttcc tgatggaaaa
    14641 gaatttgtag taagaggaat ttgtgaagga atgataggat ttgaagaaaa aggtaaaaat
    14701 ggttttggat atgatagctt atttatagta aaaggatatg ataagacttt tggtgaaata
    14761 cctagtgtta taaaaaattc aatcagtcat agagcaaacg cactaaaact tatgaaacaa
    14821 gagtttataa aaagggtagt gaagtaatga aaataggcat agtaagcgac acacatatga
    14881 tgattaaaaa tatggaaaaa acaataccat atttgaaaga atgtgaccta ataattcatg
    14941 caggagataa ctttactgat tcaagatata ttcatagcat gactaatgta ggtattattg
    15001 cagttaaagg taattgtgac tttgatgctg ttgaggaaga agttgtcttt gaagtggcaa
    15061 ataaaaccat attcttatgc catggagata agtatggcgt aaaatatgga actcatatgt
    15121 tagaaaaaaa ggcaactgaa gttgatgctg atatagttat atttggccat acccataccc
    15181 catttagaga aattaaagat ggtgtacttt acataaatcc tggaagcact tcacttccaa
    15241 gaggagtatc gtataaaagt tttgtcatta tggatataga agaagatgac ataaaaattg
    15301 aagaaatacg catataaagg tgtatttatc taaaaaaatg gcaaaaaacg ctcaaaaaat
    15361 taaattttat caaataagta caacgatatt aatggaacac ttgtaaataa aaaaacttat
    15421 gtgatataat taagtggttg aattataaat agaatgaatt ataataccgt tatcgggtat
    15481 tacataaata tcactaggag gattagaagg atgaaagcag aactaattaa aaaagagggt
    15541 aataaagtta ctttaaaaat aacagtagat aacaataaat ttgaagcagc agtaaataag
    15601 gcttataata aaagcagaaa taaatacaat atacctggat ttagaaaagg taaagctcca
    15661 agaattgtta tagagactca atacggaaaa ggtatattct acaatgatgc tatagaaata
    15721 ttattcccag aagtttatcc agaagctatc aaggaattag acatagaccc aatagacaat
    15781 ccagatttag atatagaaga aataagtaaa gacaatgggt tggttatggt tcttaatgta
    15841 gaagttaaac cagagtttga attaggaaac tacaagggta tagaaatagc taaggttgag
    15901 aatactgtaa gtgacgaaaa tgttgatgct aaactacaag aaatggttga aaaaaatgcc
    15961 agattagtaa gtgtagaaga caaagcttta gaagatggag atacagctat aatagacttc
    16021 gaaggttttg aaaatggtgt agcttttgat ggtggtaaag gtgaaaatta taacttagtt
    16081 ataggttcaa acacatttat accaggattt gaagaacaat tagtaggtaa aaaagctggt
    16141 gaagaagtag aagttaatgt tactttccca gaagaatatc actcacaaga tttagctgga
    16201 aaaccagttg tatttaatgt aaaaataaat gatgtaaaag taaaagaatt atctgcactt
    16261 gatgatgaat ttgctaaaga tactagtgaa tttgattctt tagatgaatt aaaagcagat
    16321 gtaagagcta agttagaaga agaagctaaa aatagagcag atgcagaaac tagaaattca
    16381 gtagtagaaa aagttgctga aaatacagaa atagaaatac cagaagttat gatacaacat
    16441 caaatagata atatgttaaa tgaacttaac taccaattac aatatcaagg atttggatta
    16501 caacaattac tagagatgac tggtagaact atggaagaat taagagaaga gagaaaagaa
    16561 gatgctaaaa aattagttaa gtcttcttta gtattagaag caataactaa agctgaagga
    16621 attgaagcaa cagaagaaga atttaaggct gagttagaaa aaatggcttc tgcttacaat
    16681 atggaagtag aaaaaataga agcttcttta agagatgccg acaaagaaga tataaaaggt
    16741 caaataaaaa taagaaaaac aatagattta ttagttgata atgcaacaat agcttaataa
    16801 aataaacgct aactaatata gttgacgtta tgaaatgttt agtatataat gatgtgcaaa
    16861 tataatttag ttaaatgcgt ataataattt acagtagctt aaaaaaagct actgtaaatt
    16921 atatagaata aagggggtat gaaaccatgg cattagtacc tgtagttgtt gaacaaacag
    16981 gaagaggaga aagatcttat gatatttttt ctagactatt aaaagacaga ataatcttct
    17041 taggagatca agttaacgat gccacagcag ggcttatagt agcacagcta ttatttttag
    17101 aagctgaaga cccagacaaa gatatacatt tatatataaa ttctccagga ggaagtataa
    17161 cttctggaat ggctatatat gatactatgc aatacatcaa accagatgta agtactatat
    17221 gtataggtat ggctgcttct atgggagcat tcttgttggc agcaggagca aaaggaaaaa
    17281 gactagcact accaaatagt gaaataatga tacatcaacc attaggtgga gctcaaggtc
    17341 aagcaacaga tatagaaatt catgcaaaga gaattttaaa aataaaagaa actttaaatg
    17401 agatattatc tgaaagaact ggtcagccat tagaaaaaat taaaatggat actgaacgtg
    17461 ataacttcat gagtgcacta gaagccaaag aatatggttt aatagatgaa gtatttacaa
    17521 agagaccgta gatagagaga ggtgttaata tgtctaaata tgaagaaaaa cgacaattaa
    17581 agtgttcatt ctgcggtaaa aatcaagatc aagtaagaag attaattgca ggtccaaatg
    17641 tatatatttg tgacgagtgt gttgaacttt gtgacgaaat tattcaagaa gagatagaag
    17701 atactataga tgaggatact acatctttac caaaaccaaa ggaaatgatg gagattctaa
    17761 atgattatgt aataggtcaa gaaaaagcta aaaaagcttt atcagttgcg gtttataatc
    17821 attataagag aatatacagt aaaaaatcta gtagtaagga tatagaaatt caaaagagta
    17881 atatactttt attaggacct acaggttctg gaaagacatt acttgctcaa actctagcaa
    17941 gaactctaaa tgtacctttt gcaatggcag atgctacatc attgactgaa gctggatatg
    18001 ttggagaaga cgttgaaaat atacttttaa aacttattca agctgctgat tttgatatag
    18061 aaaaagctga aagaggtata atatatatag atgagataga taagattgca agaaaatctg
    18121 aaaacccatc tataactaga gatgttagtg gtgaaggtgt tcaacaagca cttctaaaaa
    18181 tattagaagg tactgttgca aatgttccac cacaaggagg aagaaaacat cctcatcaag
    18241 aatttttaaa gatagatact actaatgtat tatttatatt aggtggagct tttgatggat
    18301 tagagaagat aattcaaaaa cgtggtggag ataaaacact aggttttgga gctaaaatag
    18361 aaagtaaaaa agaattagat ttagggaagc tatatgaaaa agtacttcca gaggatttat
    18421 taaaatatgg tataatacca gagtttatag gaagaatacc agttttagct actttagagt
    18481 tgttggatga agatgcatta atgcagatat tacaggaacc taaaaatgct ctagtaaaac
    18541 agtataaaaa gttattggag ttagatgatg ttgagctaga atttgaagaa ggtgctcttc
    18601 gtgcaatcgc taaaaaggcc atagaaagaa atacaggagc tagaggactt agaagtatag
    18661 ttgaaagcgt aatgatggaa actatgtttg aagtaccatc tagagataat ataaagaagg
    18721 ttatagtaac agagaaatct gtgaacgaag actcagtaaa tcctatcata gtacttaagg
    18781 atcaagagga aagtgcataa gatgatgaaa aggagcttta aaagctcctt tttttatgtt
    18841 aatatgctaa atataaaata ggtatcatta ttataaaaat gtgagtaata tacctagaat
    18901 accaaagacg aatacaattg caatggaacc aatttttcct ttatataaaa gataaaatga
    18961 tgctataaat gcaattattc catataaatc ccaattaact tctttggcag atgaaaaagc
    19021 tgctgataat atgaatccta gagtaattgg tctaagaact gtcaataagt tttctacctt
    19081 tttattattt ttaaatttag aaaataactt tgatataata gttagaccta ttaatgcaaa
    19141 aattgtaaca ccaatagttg cggctagaga accaaagaag ccagcagtct taaatcctac
    19201 aaatgtagca ctatttattg ctacaggacc tggtgtagat tgtgatatag caagtaaatc
    19261 caaaaactct tgattagtta taaagtgata tttagatata aattcttgct ggataaatgg
    19321 tagcatggca taaccgccac caaaactaaa tgcccctatt ttaaggaata ttaagaagag
    19381 tagtattatt tctgacatag actatataca cctcctccaa ttattaaata aatagggctt
    19441 atattgaata caccaacaga taaaactgct ataattaata gtagtatgtt agttttagac
    19501 tttggtagtt tcttgaacat gctcgtaaaa gaatacaata taagagctat tacaacaggc
    19561 gcaactcctt taaaaaatcc atctaaaatt ttagatgatg aatttttaaa gtatagatac
    19621 gataagacta agactataca aaatgatggt aaagcagagc ctaaggtaca tataagaact
    19681 cctggtatac cataaagttt atatcctaat aatacagata tattaactgc cagtactcca
    19741 ggataacttt gagctatgga taaataatca ataaattctt cctgctctaa aagtttttgt
    19801 ttgtttacaa gttcatcttg tataagagga accatagcgt atccaccacc aaatgtaaac
    19861 gcaccaattt ttaaaaaggt taaaaacaat ttcaacataa tagaaacctc caaaagataa
    19921 ataacatcta agtatactaa tattaatatt atatcataat aaaaagataa tatagtatga
    19981 tagtattcta agaatttatc attaatgttg aaaattaact tgttcaaata tataatatta
    20041 aaagcaaagc aattagacta gaaaaggagt aatgttgatg gaacaaaatt atactaaaat
    20101 agatcatgaa ttaccattaa ttcctctaag aggattagct atatttccat atatgatatt
    20161 aaattttgat ataggtagag agatatctct taaagcttta gaccaagcta tgatggatga
    20221 agaacttata tttttaactt ctcagaaaga agcagaggta gatgagccag gagaagagga
    20281 tttttatcat gtgggaacaa tctgtaaagt taagcaaatg ataaaattac caggagatac
    20341 agttcgtgtt ttggttgaag gtgtatcaag gggtagagta aagaaaatag aacaagaaga
    20401 tgggtatttc agagcagtaa tagaagaaat agtatttgat agtgataatt tggatagtga
    20461 aacagaagtt gaaattgaag catttgtaag aaatgtattt gatgcctttg aagagtatat
    20521 aaatattgga aatagggtat caccagaaat attaatatct ttagctgata tagaagatgt
    20581 agatagattt atagatacga tagctgcaaa catatatcta aaatcaagcc aaaaacaaga
    20641 aatattagaa gaatttgata ttagaaaaag acttgaactt atttattcta tattattaga
    20701 ggaaatagat atattaaaaa tagaaaagaa aataacttta agagtaaaaa aacagatgaa
    20761 taaggttcaa aaagaatatt atcttagaga acaattaaaa gctatacaga aagaattagg
    20821 tgaagaagaa gatataaact ctgaagcaga tgagtataga gaaaagctta aaaaaataaa
    20881 agcacctaag acaactaaag agaaaataga aaaagagata gataaatttt caaaaatatc
    20941 ttctatgtct ccagatgtat ctgtaagtag aaactattta gatactatat tctcattgcc
    21001 atggaataaa gagacaaaag ataaattaga tataactaag gcaaaagata tattagatga
    21061 agaccattat ggattagaaa aagttaagga gagaatacta gagtatctag ctataagaac
    21121 tcttgctaag tcacttaaag ggcctataat atgtctagtt ggtcctccag gaacaggaaa
    21181 gacatctata gtaaaatcaa tcgccagagc attaaataga aaatttgtaa gaatatcttt
    21241 gggtggagtt agagatgaag cagaaataag aggtcataga agaacttatg taggttcaat
    21301 tccaggaaga ataattaatg gggtaaaaga agcccaaaca aaaaatcctg tattcttatt
    21361 tgatgagata gataagatgg cagcagatta taaaggtgac ccagcttcag caatgctaga
    21421 agtattagac ccagagcaaa ataaggattt tgtggaccat tacttagaaa taccttttga
    21481 tttatcaaaa atactatttg ttacaacagc aaatagtcta agtaatatac caagaccatt
    21541 attagataga atggaagtaa tagaagtatc tggatatata gaagaagaaa aattaaatat
    21601 agcaaaaaaa tacctacttc caaaacaaat taaagaacat gctttaaaag aaaactttat
    21661 aaaaatagat gatgaaactt taagaagtat aatcaaccat tatacaagag aagctggtgt
    21721 aagaacacta gaaagaacta ttggtaagat ttgtagaaag gttgccaaaa aatacgttga
    21781 agacccaact cttgaagagg ttgttataaa taagtctgac ctagagacat atcttggaaa
    21841 agacatgttt aagtatcaac ttgcagaagt taaccctcaa ataggattag taaatggtct
    21901 tgcgtggaca gaagttggtg gagttacact tgaagtagaa gtaaacgtac ttaaaggtaa
    21961 aggcgaaatt gtattaactg gaaaattagg agatgttatg aaggaatctg cgaagacagg
    22021 tatctcttat ataaggtcaa tagtggacaa gtttgatata gaccctgaat tttataagac
    22081 gaatgatatt catatacaca ttccagaagg tgctgttcca aaagatggac catcagctgg
    22141 tataactatg gctcttgcag taatctcagc acttactaaa agacctgtgc ctggaaatat
    22201 agcaatgaca ggagagataa ctttaagagg tagagtctta gcagtaggag gagttaaaga
    22261 aaagttatta gcagctcata gagctggtat aacaaaagta ttaattccta aagaatgtga
    22321 agctgattta gatgaaatac cagaaaatgt aaaagaaaaa atggagtttg tgcttgtaga
    22381 acatatggat gaagtattag aacaagcttt attaaagagt ggtgaaaata atgaaaatta
    22441 gaagttcaga gataactatg agtgctgtga ataaatcaca gtatccagca gaaggaatcc
    22501 cagagatagc attggctggt aggtcaaatg ttggaaaatc atcaataata aatacattgc
    22561 ttaacagaag aaactttgct agaaccagtc aaacaccagg aaaaactaga actataaatt
    22621 tttatctaat aaataatgaa ttttattttg tagaccttcc aggctatgga tatgcaaaga
    22681 tagctaagtc tgaaaaagaa aagtggggag gtataatgga acgatatcta gaaagtagac
    22741 aagagttatg ttctatattc ctacttgtag atattagaca tgagcctaca gcagatgata
    22801 agcttatgta tgaatggata aagcactttg gatataactg tgtagttatt gcaactaagg
    22861 cagataaaat atctagagga caatatcaaa aacacataag tataataaga aaaaaattgc
    22921 aaatggaaag tagtgaaaaa gtaattcctg tttcttcact aaaaaagact ggtgttgaag
    22981 aattatggga agagattgta aatcaataca atcaacatgg atatgaaata acagtagatt
    23041 aaatttaaaa tgttaaatat tgacataaga tttatttgaa taataaatgt aataatagtt
    23101 agttttaatt atattaatat taaagtaaaa tataggatgc cttaaaatag ttttaatgaa
    23161 cttttttttg gcatccttat tgtttataaa caataaatat cagtaatttt gctaaatact
    23221 aataaatttt agtaaggtat gggaaaatta gtataaaata gttaaactaa aaaattattg
    23281 atttatgtag tcagtaataa atagcattta agaaatagat ttatctgttt tcctcataaa
    23341 gaaaacagtt agtataagcc ctacaaaaca tactgatgct gcaatccaat aagactgatt
    23401 gctagctata acataggctg atgtgtgact tttaaacttt tgattgtatg taagactttg
    23461 tgcaaaaaaa ctactaaaaa atgctgttcc aagagcaaat gataaattac gaatagttgc
    23521 aagaaaactg cctccgtacc cctgtttttg aggaggaaca tctgctaaaa tactactatt
    23581 gtttggtgaa ctaaatgctc ctagcccaag tccaaaaata actaaacata caattaaaag
    23641 gtacaatgga gaatctgctt ttaaaagtgc cattccaact agtgagatgg tcataaatga
    23701 aaaagatgct gttagaatgt tttttgcacc gtgtttatca gaaagtgaac ctgcaatagg
    23761 tgctataagt accataataa gtggatgtac agtcattaca taaccagcca tcataggaga
    23821 taaatttttt aattgttcta aataaaatgg aagtagaaaa gatactgcca tttgaggaaa
    23881 ataacaagct actccaatga tattgcctac agtaaagttt ctatttttaa atagagggag
    23941 gtcaataaag ctttgttcaa attttacttc tcttaatgcc aacaataaga agattataag
    24001 tgctgttata cctaaataca atcttgtatt acccttctca ttcattgcaa gtaatataag
    24061 cgtagtgcaa gatgcgaaaa gtagaagacc tggaatatca aagctcttgt tttgttcatc
    24121 caaaacaggt ttaggcagaa atttaatgcc aagaaagact gctataatac caatagggat
    24181 attgataata aaaatagaag gccatccaaa atgttctaaa attatacctc caattacagg
    24241 accactcata tttcctatac caactacagc tccagtgatt cctatagctt ttcctttttc
    24301 atttgctgga aatgcattgg aaactatacc aagcccagta gccattaaaa tacttgctcc
    24361 aactgcctga aagattcttg aagaaattaa tgttgataaa ttgtttgaca tactacacag
    24421 aaaagaacct attgtaaata taaaaaatcc taatgtgtat agacgatttg aacctacttt
    24481 atcgccaagt tttccgaaaa ataacatagt agctgttaca acaagcatat aagctgttac
    24541 aacccactga atttgtgaca tgttaatttt aaagtcagca gcaattgtag gatttgcgat
    24601 atttagtata cttccatcaa gagtagacat aaatacacca atacagatga taatcaatac
    24661 tatccatttt tgtttcatga taacactcca atcttaatag attttataag tacgatataa
    24721 ttgtaaaata aacaacaaaa aaagcacctg tatattaggt accttgaatg tgtctaattt
    24781 catgaataaa tacggtagtt gaaggatttg tatagattac tcatgtaaaa ttacataaag
    24841 gtactcatat actattttct tctaccatcc agattttact gtcggctttg gaatatgacc
    24901 aaattatgcc ataaggctcg cgggctttta ccgccgatag gtaatttcac ccgtttatga
    24961 agaaaaacag atgtattttg taatattatt ttaacataaa attataatgg aaacaattgg
    25021 atttaagtaa tatccagctg tattacaata caaatatttc aatatggaca cttaaattgt
    25081 atttatagat agaataaggc acagtatgag aatctataat aattagataa aattaacctg
    25141 ttattagtat tagatggcaa gttttaaatt tgtcaaaatc agaaagctgt aataaaattc
    25201 ttataatatg aatatcttaa ttaagagata ttgtaaatta tgaggaggcg ctaatatgga
    25261 aggaaaggta aagaaggcat tcccgggtgg aaatacatca caaggctcat attctttttt
    25321 tgactatata attcctgaaa atgtaaaaag agttttttgt ttaaagggtg gacctggagt
    25381 aggaaaatcc tcacttatga aaaaaattta taaagagttt ttgcatagag gatatgatat
    25441 tgatttatat cattgtcctt ctgacccaag ttcgttggat ggtttagtta taaagaaatt
    25501 aggcgtggta cttatggatg caactgcccc acatactatg gacccaaagc tacctggagc
    25561 tttggatgag ataattaatt taggtgatta ctggaataca gaagaattag aaaagaataa
    25621 acaagaaata tcagtttatg ataaagatat tagtaattca tttaaaagag catttaagtt
    25681 tttaaaatca gcagaaccaa tctttagaga catagaagaa aaatattctg attgtatgga
    25741 ttatggcaaa gtaaatttgg tcacagaaga atttataaaa aggttatttg atggagttaa
    25801 aagcacagga aatctaaaaa gagaaaagca tttatttggt tcagctataa ctcctgtagg
    25861 gtgtttagat tatacagaaa acattttaaa agatgtaaag aaagtctatt atttagatgg
    25921 agaaatagga actgggaaaa ctacattatt aaacaaagta tataagaaag ctattgaaaa
    25981 aggtttaaat gtagaagttt atcattatcc acttattcca gagaaaattg aaactgtact
    26041 attaactgat ttagatatag gcataactat aagtacgact tttaaagaag aagatacaat
    26101 tgatttaaat cagtttttaa atagagaaaa actacttaaa tatgaagaag atattaaatt
    26161 tgatgaaaaa gttttagatg atttaatttg ttgggcagta ctaaatctta agagagccaa
    26221 gtctaaacat gatattatag aaagttacta tagtcctaat atgagatttg atgaagtagg
    26281 gaaattaaga gataaattaa ttaaaagaat tttaaaatat gaggaaaact tataaacgta
    26341 tatgtttaaa attaatgtta ataatgatat aatttttagt aaattggtgt agctttgagg
    26401 ctatgccatt ttactttttt ttgctaaaaa atgatattaa taatctccaa agcaattatt
    26461 tgtttttcat aattattatt tcctttccta atttatgttt ttttataata tcaaaacaat
    26521 gtttattgaa agtttatata attttaagaa catctaaaaa tgcatacttg atattggtaa
    26581 attagtgata ggagttcaat gtatgatatt atatactaaa gatgaatttt aatatataac
    26641 tttgatataa taagcatatg taaatgttta atatttaatt aaagaaaatt atcatataaa
    26701 aataaatttg taaggagcaa tacagtatga aaaaacaata tgtgtttata atactagcaa
    26761 gtactatttt aggtatactt gttggaacca tatttagaga ttatagcaat gaagaaacag
    26821 tttatacaag caaagacaag tcaataagaa aagaaataaa aatgacaaga aaatctataa
    26881 aagaattaac caaagagaaa gacaagttag ataaagaaat taattcaatg aaacaaaatc
    26941 aaaaaaattc tgaagaaata tcagaaatag aaacacttag agatagtcta tcatattcag
    27001 atgtaaaggg tagtgggata atattaagta tagatgctat agatgaaaaa acagggaata
    27061 tagctaatag tatagactat aataagattc ttgtaaaagt tataaatgaa ttaaaagtaa
    27121 acggaggaaa atacatagaa ataaatggtc aaagaattaa tcaatattca gaagttgtac
    27181 ttgcaggaag ccatataaat ataaatggag ttcctgtagc acaaccatat gaaataaagt
    27241 gcattggaga taaggagaaa ctatctaatt atgtagataa aggtagtaaa tatttagata
    27301 gcatacaaac caattatcca atgaaaataa gcataaaaac tgaagaaaat atctctatga
    27361 aaaaaatgaa tatacctaat aaattaaagt atataaaggg tgattaagat tatattagag
    27421 gaaagcagtt tgccaatgtt aataccagaa aaacttgcaa aaaaatataa tgtatttcca
    27481 gttgagtttc aagatgataa tttagttgta gaaatagaag atgaggatat ttatgctcta
    27541 caggacttga gattagctac aggtaaagaa attatattga agaaggagaa aaaagaactt
    27601 ataagtgaaa agattgaaaa atattataat agctttgaac ttgatgagga ttatgcaaaa
    27661 aaattatttg aaaatctttt agaaaaagct ataaaagaaa acgcaagtga tatacatata
    27721 gaaccattta aagaacattt aatagtgaga atgagggtag atggagaact aaaggaagta
    27781 tcaaagtttt tagttgatgt ctatccatca ttgtctacgg ttataaaatt aaaggcatca
    27841 atggatataa cagaaaaaag gcttcctcag gatggtaggg tggatataaa gttaggtgga
    27901 aatttaattg acattagagt ttcatcaata ccaactgtct atggtgaaaa aatagttttg
    27961 aggatactta atagagatac ttttttaaaa gacaaattag aacttggttt ttcagaggaa
    28021 gctataaaat ctataaatcg tataataaat aaaagagcag gaatattatt agtgacaggt
    28081 cctacaggta gtggaaagac tacaacagta tattccttat taaatgattt aaaaggaata
    28141 aataaaaata ttatgactat agaaaatcct gtagagtata agatggatgg cataagtcag
    28201 attcaagtaa attcaaaagt agggttgagc tttgatgtag gtcttagagc gatattaaga
    28261 caagacccag atataataat ggtcggagaa ataagagatg cagaaacagc taagatagca
    28321 gtaagagcag ctataacagg tcatttagtt ataagtactt tacatactaa tgatgcagta
    28381 tcttccatag ctagattact agaaatgcaa ataccaccat atcttttaaa tgcttctctt
    28441 ataggtgtta tatctcaaaa gttggttaga aaagtctgca atcattgtag ccatgagatt
    28501 atgataaaag ataattttag tggagatgta aatactaagg tagctgtagg gtgtgaaaaa
    28561 tgtaatgata aaggatactt tggaagaaca gcaatttatg aaatcttaga aattaatgat
    28621 gatataaaga catgtataag aaatatggag gattctagta ctattaaaga tgtagcacaa
    28681 aataatggaa tgataacatt tgaagatagt tgtaaaagac taataaatga aaaaataacg
    28741 acattagaag agtgtttagt agtaaatgaa atgatttaac tgaattgtat agttaaactt
    28801 cgtttggtca gttgtcttta tttaatctat tttaattaag atgtttgagt ataaattata
    28861 tttaagtgaa ataaaattaa atgcattaaa ttagaaaata ttaagtttat ttaattgtaa
    28921 ttaatataat ttggaaggta ttatagtaca tgagattatc taaaaatata ttttataatt
    28981 tagaaaagtt ttatttagta ttaaatagaa tcagtgataa agaaattaga gtaatttgca
    29041 aagagatagg catattgctg gaatctggat gtgaaattac aaaaatattt gaaatcattg
    29101 agagtcagtc aagtaaaaaa gcaaagaatt tgcttagtat agtatcaaat catattcaaa
    29161 aaggtaactc aatagcagaa tcatttcaaa taacaggtat attttctaaa ttctttataa
    29221 gtatgataaa agcaggagaa actagtggaa acttagatat aataatgagt gatttatcta
    29281 attattatga taaagaatac aaattaaaaa tgaaaataat aactatttca atatatccaa
    29341 taatacttat aatattatct atcttatcaa tgttgtttat attcgtattt gtaataccta
    29401 attttcaggt ggtttttacc aacaatggaa ttgaaccacc tttaataact agagttttaa
    29461 tgggaatatc tacagtggtt acaaataact tagcttatat aatatttagt tttatattgt
    29521 tttcaatagg aagcatttat ttctttataa caaatgataa tataaaaaaa ttgattaatt
    29581 cgttaaaatt caaaatacct tttataaaaa aaattaatca gttagtagct acaacaagat
    29641 tttcaagggc attgtccgtt ttaattagca gtggaataca aattgtggaa gctatagaca
    29701 tgtcagctag tgttatagac aacgaattta tttatgaaag gttaaagatt tccaataatt
    29761 atataaaaaa aggaaatagc ataggtaaat ctttgaattt agcaaatgtt tttccaaaat
    29821 tatttatttc aatggcaaat acaggagaag aaagtgggaa actagataaa agtctaaatg
    29881 taataaataa attttatgaa aatgaattaa ataacaaaat agagcaaata atgaaattta
    29941 tagaacctat gctaattgta tttatgggat taattattgg tacttttatt ttagcaatgg
    30001 ttgttcctat gtttgatgca attactaatt tttagagaga aaaataaatt ttattagatg
    30061 aaacttttga aaaggagaga atatttaatt gataaatttg ataaataaaa aacgaaaagg
    30121 atttacactt gttgaaatga ttgtagtagt aactatttta ggcgttatat ctagtatagc
    30181 attagttaag tatagtaagg ttcaagaaag tgccaaatta aatgcagact atacgaatgc
    30241 tgctaatata gtaaccgcag ctagcatggc aattaatgat gatgaaaaga caatagactc
    30301 tctaagtgta gagacattga aggaaaaggg atacctaaat actgttccag ttcctcagag
    30361 tacatcaggt aaattcgaac ttgtcataaa tgatagcgga acagatataa gcgtaaatat
    30421 aaattctaag caattttatc caaaataata ttttatattg attgctgtaa gttttaaaat
    30481 tatttttatt taagaaggag atggctataa ttaaaaaaat tctaaactta gaaataaatg
    30541 atgattatat aaggcttgta tttttaagaa aaagtcataa aaaagttttt atagataaat
    30601 caattataaa agagattaac tttgatataa aaaatgatag taattcgatt gagaagatta
    30661 aatatataga agatgtagtt agatttgtaa aagaggagat tggtaaatca aaaatattat
    30721 ataaaaattt atactttaac attcaaattc aggatatagt cattagaaat gttgaagttc
    30781 taaatactaa gaaaaaaaga gacatattaa gcatgattga atttgaaata acacaataca
    30841 ttccaataaa cataaataac tattcaataa aatataaagt tataaattct aaaaatgata
    30901 aattgaatat acaagtaatt cttttcccaa agagtattat tcaattaatt aagaacattt
    30961 cagaaaatct aaatatgaat ccaaagacta taaatataaa ttttgatata ttacaaaagc
    31021 ttataaactt aaatttaata gagaattttt cagaagaagg ggtatttata gaatgtaaaa
    31081 aaagtcagtt aattataaat ataacaaaag ataaaaaaat aagagaaaca tacttgctat
    31141 caaaagaggg aaaatcatat gagttcataa aaagattatt aagggatttt aagtatgttt
    31201 attactatgg tatagaaaat tgttttattg aagaatactt taaatataca gataaatcta
    31261 ttgaagtaaa tccattaaga ttaaaagata aagtaaaagt agaaaataat tttgatgcag
    31321 atttagaaga caataggata aattatataa gtaatatagg aatgattatt taagtgaaag
    31381 tggataaaac gataaatttt cttaatggat ttaaaaaaac taaaccatgt aataaaatct
    31441 ataaaataat aatagtctta attttaacgt gtattttatt taaatcattt atgtatgtga
    31501 aatctataaa tgaacttgaa gattatataa gtcaaaataa gctagtggat aaatcaagac
    31561 acactttacc aacaacagat aaaactagag agaaattttc taaaataaaa cttaatgaaa
    31621 taaaaaaaat atatgaaaca attggaataa atattattac taaaatatcg atagataaaa
    31681 ataagctaga gattgaaggt gtgtgtagtg atttagatgt tttaaacaat ctaaggaaaa
    31741 taaagtacat aaaatattat tacatagaca gtattgtaag agatggagaa tattatagat
    31801 ttagaataaa aagttagatg ggtgattatt tttgagatta aataatagag aaaaaaatct
    31861 acttaaaata ttgttaatat tagttattat attggcaagt gatatagcag taagttattt
    31921 ggaagaaaac ttaactagtt tgaagaatga aaagcaaaat ataataaata aaataaataa
    31981 tcaagataat tatcaaataa aggataataa gcatatagat gataaatata tagaaagtgt
    32041 tgaatcaaat aaaaatgaag aattaaaaaa taattttaat aactcatttt tagataaaaa
    32101 aacaaataaa gatatagtgt tattagtaga aaatgaactg agagatattg tgaatgtaga
    32161 atatattaat caagaaagta gtttagataa tcacaataaa aaacaagtta atttagaaat
    32221 aaaagttaat ggagaacttg caaatttgtt gaaacttgaa ggtgtaatga agaagttaag
    32281 actaaataat aaacttgaaa agatagaaat taataaatta aaagtcaata attttgatgt
    32341 tcaaaatatt ccaaatgaat ataaacaaga aaaagacaat attgttgaat gtaaaatgac
    32401 agttagagtc atataacaaa aaagaataga taaagtaaaa aactattatc aattaataaa
    32461 attttataga ggaattagtt ttacgataat tcatttctac aagagggatt gttgatgatt
    32521 ttatcgaaat cctaattaaa gttaatatat tgtaccaaaa tataagtgaa tttaatagaa
    32581 tatttactta tactatagat tgattaataa aaaaatggta taataacaaa ttgtacttta
    32641 atttaataat aagggagtgg aagtcatatt gttagggaga aatgagctat gcccttgtgg
    32701 aagtggcaaa aaatacaaga aatgttgttt aaataaagat attgtttcag aaagagcatg
    32761 cagaaaaact gaactttcac aaaaacaata tacagattta tattcaaagt tatatgatta
    32821 ttcaagacag gataagttta aagaagaatg tgaaaaagct aaagaaatgt tttatataat
    32881 gcaaaatgat agtgtaaatg aaaaatttga aagatttttt aatacttatt taatacaaga
    32941 ccacataatg gaaaataaaa aggttatgac agttggtttt cttgaagaaa atggtgcaaa
    33001 cctaagtcaa tcagaagtat caatattaaa aagtatgttt gaatcatacg taagtattta
    33061 tgaggtaaaa gaagtttctg ctggaaaaat agtattaaag gactgtttaa gtggaaatga
    33121 actttgtaca gaagatgtaa aacttctgag aagttttaaa atcggaagtt gtatgatagc
    33181 tagaatagtt gatattgaag gaactaacat attgatagat ataactataa gtatatcaaa
    33241 tgaggttaaa gatataattt taaaggatat tatgaatcta ttcaatcaat atcaagatgt
    33301 atacaaagat atgaagtcgt tcttaattta tcatacacat atactataca agtatataca
    33361 acagctttta gacccaagta tagctgaata tttaaagaga gaaagagata aaaacgatac
    33421 aaaagttaat tcagaaaatg aattagctga agaagaatgt aaagttttag atactcttaa
    33481 agaaaatgta gaaaaagaaa atatagatgc atgtatagag ttttggaatg aatataagaa
    33541 aaatcatgaa aatataaaag gagctgaaaa tggttgggca gctgctgttg aatactatgt
    33601 taaaaaagaa gcaggacaac ctataactca agttcaaata tcaaagaaat acgatataag
    33661 ctctagtaca ctaggaaaga gatacaagga tttaaaaata tcttaattaa ttataaaaag
    33721 ctttaagtca tagataaaga atctatagta taatatatgt ataatgttta gaaagaggta
    33781 ttaataatga gtcttagttt aggaaatgca attgctgtat caacgaactt tataaaatct
    33841 aacccagctg tacctgtgta caatggagaa gaaatatatg cagttattta tcatatagaa
    33901 ataggatata ataatggtgt atcacctgat accaaacaat atataataat agactataaa
    33961 gagtttgatt tatctatgga taaaaaaaat gaactaataa atattgctaa agagtactgt
    34021 aaatctaaag gtattgtaga aaacgaaaac cttgaaataa accttttaga caatgaagaa
    34081 gcagagaact tcttagaaaa ggtgtttaat aatatgaagg ttataagagg tcttataact
    34141 aaaaaaggat aaataaatgg actatatagg aatagaaaat ataacacctt atgaaaatac
    34201 atatgaattt agtgtatatg aatatgatga tgaaatcacc ttaggtagtg aaaagttata
    34261 tgtatgtgaa ttaagggttg tattgattaa agttaattct ctgtatgttg aaagattgca
    34321 taaatcagtt gaagcaatgg tcttagtaaa aaacttgaaa aaagatttag ataaaacact
    34381 tgttgtaaac aaaataaaga attttgtgct agatgagatt tgggtagaaa atctagtaaa
    34441 agagaatata gaagttatat ttgtagaaag ctaggaataa ccctagcttt tttatattac
    34501 aatctaagag aggaaaacaa tatgttaaga gtatctaatg taaaacttgg aattgataaa
    34561 gatatatctt tattaaaaga tgttatactt aagaagctaa aaataaaaga aaaaaactta
    34621 gtcaaatata gtatatataa agaatctata gatgctagaa aaaaaggaaa aatggaattt
    34681 gtttattctg tagatgtaga agttaaagat gaaaataaaa tacttaactc aaaaataaaa
    34741 gatgtaacaa aaataaagga aatcagatac gtaaatgtac ctgtgggtga taaaaaatta
    34801 aagaataatc ctttggtaat tggtagtgga ccagctggtt tgtttgcagg tctgctttta
    34861 gctcaaatgg gttatgaacc aatcatactt gaaagaggtc ttgatgtaga cagtagaacc
    34921 aaagatataa gtgatttttg gacaactaga aaattaaaaa ataattcaaa cgttcaattt
    34981 ggagaaggtg gagcaggaac tttttcggat ggtaaactta caactagaat aaaagatata
    35041 cgatgtagaa aagtattaga agagttagtt cagtttgggg caccagatga aatattatac
    35101 tcacataagc cacatgttgg aacagatata ttaaaaaatg tagttaaaaa tataagaaat
    35161 gaaataataa gtttaggtgg tcaagttaga tttaattcaa aagtaacaga tatagttgta
    35221 tcaaatgatt ctatacaatc tgtaataata aatgatactg aaaaaataga tacagataca
    35281 attatattag ctgttggaca tagtgcaaga gatacttatg aaatgttgta ttcaagaggt
    35341 ataaaaatag ttcaaaaacc ttttgcaata ggagctagag ttgaacatcc acaagattta
    35401 ataaataaag cacagtacaa ggatttttat aatcacccta ggttaggagc tgcagattat
    35461 agacttatag agcatgtttc aaatggaaga acagcatata ctttttgtat gtgtccagga
    35521 ggtagtgtca tagcatctgc atcaaatgat tttgaaatag ttacaaatgg tatgagtgaa
    35581 catgcaagag ataaaataaa tgcaaatagt gcatttttag taaatgtaac gccagaagat
    35641 tttaagagtg ataatccact agcaggtgtt catttccagc agaagtatga aaaattagct
    35701 tttgaattag gtggaaaaaa ttataatgca ccagttcagc ttgttggaga ttttataaac
    35761 aataagactt ctttgaattt aggaagtgtt gagccatcat atagaccagg atatacactt
    35821 acaaatttaa gtgaatgttt gcctagcttc gtaacagaga ctatgagaga agcgtttata
    35881 tctcttgata aaaaattgaa tgggtttgca atgtatgatg ctgttttgac gggagtagaa
    35941 actaggtcat catcaccaat aagaatagtg agagatgaaa atactttaga atcagtgagt
    36001 attaagaatc tatatccatg tggtgaaggt gctggatatg caggtggtat agttacagca
    36061 gcagttgatg gtataaaatg tgcagaaaaa ataatacaaa aatacaccaa taatacaaaa
    36121 atttaacata aacttaatga ttttttaata tttaacatga atttaacatt atcactccta
    36181 taatttgata aaatacaata ggttataaaa tttttagata cagtaatctc tttacaagga
    36241 ggaaataatt tgaacattat aataagtcta atgggtggat taggtctgtt cttatatggt
    36301 atgaacctta tgggagaagg tcttcaaaaa tctgctggga caaagctaaa aaagataata
    36361 aaactactta ctagtaacct atttatggga gttttggtag gtacaggagt tacagcagta
    36421 atacaaagtt caagtgctac tactgtaatg gttgttggtt tcgtaaatgc aggaataatg
    36481 actttaaaac aggcaatagg agttataatg ggtgctaata tagggactac tgttacagcc
    36541 caattggtat ctttcgactt aactggtatg gccccagtag cattaggtgt aggtattata
    36601 ctttatttat ttggaaataa gcctagaata aaaaatatag ccgaaatact tataggtttt
    36661 ggtatattat ttacaggtat ggattttatg aagatggcag tggagccact aagagactat
    36721 caaggattta ctgacttact tgttacattt ggtagatatc cactacttgg attattattg
    36781 ggatttggga ttacagcaat aatacaaagt tcaagtgcat ctatgggtat gttggttgca
    36841 ctggcagcag aaggtctagt gccactatca gcagcactgc caattcttta tggccaaaat
    36901 attggtacat gtgtaacttc actgttatca agtattggtg ctaacaaaaa tgcacgaaga
    36961 gcagctatga tgcatctaat cttcaatgtt ttaggaacgg tgatattctt aatattcttg
    37021 aataaaccag tagtaagcat ggttacaagt tgggatccaa gcaatgttgc aagacaaata
    37081 gccaatacac atacattgtt taatataata tctgtattga tacttttacc atttacaagt
    37141 cttattataa aactagctat aaagcttgtt cctgatagag caggtgatat agatgaagat
    37201 gaaactaaaa ctattaaata tatagatgat agaatgatag aaactccttc tatagctctt
    37261 gcaaatacga taaaagaagc acttcgtatg ggtgagaagg cgaaggaaag cctaaatgca
    37321 tctatggaag ctttagtaga acattcaact gaaaaaatag ataagacata tagacgagag
    37381 agattaatca atgatttaca gaaagctatt ttaaattatt tacttaaact ttcaaaagca
    37441 gctttagatg atgattcaag agaagttgtg gatactttgt ttaatacagt caatgatata
    37501 gaaagaattg gtgaccacgc taaaaatata gctgaattat cacaagttgc aatagattca
    37561 aatatttatt tctcagaaga aggacaatct gaacttgatg ttatgtataa tagagttgtt
    37621 tcagcatata cttatgcatt agaatcaatg aggagcgata atgttgattt agcttgcaag
    37681 gttataaaaa ttgaagaaca agttgatata atggagaaat cttgtagagc taatcacatg
    37741 tatagattga ataataatct atgtagtata gaaaatggag ttatttattt agatgtaata
    37801 agtaatttag agagaatatc tgaccatgct gtcaatatag cacaacaagt tatagcgaag
    37861 agattaggaa atgattaaaa ataattaatt taaaataata taaaatttaa aatagaggat
    37921 aaataaaaga gaaactttaa atgatacgtt tagagtttct ttttttgtaa atttatttga
    37981 ctaaaatttt taaatttata catagttata gtatttagaa gaatcgattt cctgataata
    38041 aatgatgaga tatgtatgat tttgtggcaa acttttaaaa aaaacaccta tattttgtgt
    38101 tataatctct ttaaaacgaa tatgctgaaa agagggattt aaatgggaaa aataaatttt
    38161 gattatagta aagctacaga gtttttttgt caaaacgaaa tagatgtcat gcaaccatat
    38221 gtagatgttg cgcacgacat gttacataat aaaactggtt taggaaatac ttttttagga
    38281 tggatagacc ttcctaaaaa ttatgataaa gaagagtttg acagaataaa aaaatctgct
    38341 gataagataa aatcagactc agatgttctt ttggtaatag gtataggagg ctcttattta
    38401 ggttcgagag ctgctataga tatggtaagt cattctttta ggaatggact taaaaaagag
    38461 caaagaaagg ctccagaagt atattttgta gggcataata taagctcaac atatattatg
    38521 gatttattag atataattga aggaaaggat atttctgtaa atgtaatatc taaatcagga
    38581 actactacag aaccagctct ggcttttaga atatttaaag attacttaga gaaaaagtat
    38641 ggtaaggaag aagcaagaaa gagaatttat gcaacaacag atgcaagtaa aggtgcactt
    38701 agacagttag ctacagaaga aggatatgag acttttgtaa taccagatga tgtaggtggt
    38761 cgtttttctg tattgacagc agttggatta cttcctattg ctgctgctgg acttgatatt
    38821 gatgctatga tgaaaggcgc aaatgatgct agagaagcat ttcaaaatcc tgacttaaaa
    38881 agtaatgata gttatagata tgctgttgct agaacaattt tacatagaaa aggaaaagat
    38941 gtagagttat tagtgaacta tgaacctcaa ctacattatg taagtgagtg gtggaaacag
    39001 ctttatggag aaagtgaagg aaaagaaaat aaaggtttat tccctgcttc tgtagatttc
    39061 tcaacagatt tacattctat gggtcaatac atacaagatg gaaaaaggct tttatttgag
    39121 acagtactta atgttgaaaa ctgtaagaga aatataacta taagttcaga ggaagtagac
    39181 ttagatggtt taaattattt agcaggtaag actgtagatt ttgtaaatca taaagctttt
    39241 gaaggaactc tattggctca tacagatgga aaagtaccta atttagtaat aaatatacct
    39301 caactagatg agtataattt tggatattta gtttatttct ttgagaaggc ttgtggaata
    39361 agtggatatt tattgggagt taatccattt gaccaaccag gagtagaagc ttacaagaaa
    39421 aatatgtttg ctttactagg caaaccaggt tatgaaaaag aaaaggaaga gctagaaaaa
    39481 agacttaaat aattttaaat agttaaggtt taattaagat taaaataata taatgaaatc
    39541 atcaaaaagg agtttactca aaatagatga tttcattttt tttatgaaaa attgggaaaa
    39601 atatattttg ataatttata ttatattaag atagggagtg gtatgagatg tcaagaagaa
    39661 agaaagggat aagtttagta atattagttg ctataataag ttcaatactt agtagttttc
    39721 taacaataat tttagttaag gacaatttag taagcaaatc tacaggaagt tctacgccga
    39781 tagttgtaaa tgatgatggt aagagtcaaa atatttatca agcagttgca gaaaaagcta
    39841 cgccatctgt tgtaggtatt acaacaacta gtgttgatac tagtaatatg tttgcaatac
    39901 caactgaaac acaaggtgta ggaactggaa tcatagttga ttcaaatgga tatatactta
    39961 ctaattctca tgttatatca gatggtcaag ctacaagtgt aaatgtatta ttcaatgatg
    40021 gttcaacaac ttcaggtaaa gtagtatggt ttgaccaaca acttgattta gctatagtta
    40081 aggttgataa aactggacta acaccagcag aatttgctga tagtgacaaa gtaaaagttg
    40141 gagatatatc aatagcaata ggtaatcctc ttggattaga ttttcaaaaa acagttactc
    40201 aagggataat aagtggatta gatagaacta tacaaacaga aaaaactaat atgactggtt
    40261 tattacaaac tgatgcaagt ataaatgcag gaaatagtgg aggaccattg ttaaatcaaa
    40321 aaggtcaagt aattggtata aacacagcaa aagcttctca agcagaagga ttaggatttg
    40381 caataccaat caatacagct aagagtatag ttgaagaagt aatcaaaaat ggaaaatatg
    40441 aaaaagtaac tttaggcata aaaggtacag atgtatcaaa ttatgaagca gcaacaggta
    40501 caaaattaag tactgataaa ggtgtttatg ttgcagaagt aatatctggt tcatcagcag
    40561 aaaaagctgg agtaaaggta ggggatatta taactaaagt aggagataca gatataacag
    40621 gtatgaatga tttaaataaa aagttgtata ctttctcaaa aggtgctagt acaaaaataa
    40681 cagtaaatag aggcggaaaa gcagtaacaa taaatgtgaa tttctaaata tgaccaaaaa
    40741 gatagagact acatggtaaa gtgtagtctc tatttttatt taatttttat agtatataca
    40801 tctaaattaa ttaaaatgat gtattgttta tagtttattg ctatattttc tt
//
LOCUS       DAMJVI010000023        40700 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.023, whole genome shotgun sequence.
ACCESSION   DAMJVI010000023 DAMJVI010000000
VERSION     DAMJVI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40700
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.023"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            101..1153
                     /gene="ytvI"
                     /locus_tag="PC545_001318"
     CDS             101..1153
                     /gene="ytvI"
                     /locus_tag="PC545_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2456095.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGAI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSMKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDVEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            1243..2469
                     /gene="pepT"
                     /locus_tag="PC545_001319"
     CDS             1243..2469
                     /gene="pepT"
                     /locus_tag="PC545_001319"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDF2456096.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPKIIENYDGKD
                     IVLNEALNVITYVKDSPELEEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFDVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIAAEISEMFPADERPETTEGYEGFWHLNSIGGNVENVS
                     MAYIIRDHCKEKFENRKSIMIENIEKINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELGIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            2676..3728
                     /locus_tag="PC545_001320"
     CDS             2676..3728
                     /locus_tag="PC545_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HDF2456097.1"
                     /translation="MKIKSKKIIAFALSLGIMLPSCVYADNISKNQNAQVDMNNYLQG
                     RKSPKEEKLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKESLEDMEVLVSRIGGADREE
                     TSLLLTRQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTKTYIVGGEYSISKKLEGQLVNSERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(3981..4550)
                     /gene="yfcE"
                     /locus_tag="PC545_001321"
     CDS             complement(3981..4550)
                     /gene="yfcE"
                     /locus_tag="PC545_001321"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDF2456098.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YIESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(4575..5375)
                     /locus_tag="PC545_001322"
     CDS             complement(4575..5375)
                     /locus_tag="PC545_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HDF2456099.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            5510..6370
                     /locus_tag="PC545_001323"
     CDS             5510..6370
                     /locus_tag="PC545_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456100.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     NFDRERFDEYLNKFDIEENMHIENLSKGQSEKLMLSSALSHNAKLLILDEPTAGIDPI
                     VRTEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            6370..7056
                     /locus_tag="PC545_001324"
     CDS             6370..7056
                     /locus_tag="PC545_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2456101.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLLSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGFIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDIANTKYINLYSMTFLTIIIFIISMYFSI
                     KISKKNKFNY"
     gene            7108..7650
                     /locus_tag="PC545_001325"
     CDS             7108..7650
                     /locus_tag="PC545_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456102.1"
                     /translation="MKKFIKVSFFILGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDVGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGMNKCSAYVNVDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYIVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            7729..8196
                     /locus_tag="PC545_001326"
     CDS             7729..8196
                     /locus_tag="PC545_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HDF2456103.1"
                     /translation="MSISNVREYFKQFGKEDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLTPEEVLEFTGHAIGGVCPFG
                     LKNSIKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            8905..10041
                     /locus_tag="PC545_001327"
     CDS             8905..10041
                     /locus_tag="PC545_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDF2456104.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            10100..10882
                     /locus_tag="PC545_001328"
     CDS             10100..10882
                     /locus_tag="PC545_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2456105.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            10901..11911
                     /locus_tag="PC545_001329"
     CDS             10901..11911
                     /locus_tag="PC545_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2456106.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVQVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            11930..12727
                     /locus_tag="PC545_001330"
     CDS             11930..12727
                     /locus_tag="PC545_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDF2456107.1"
                     /translation="MSTSDVKVYENVAVEVDGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            12825..13670
                     /locus_tag="PC545_001331"
     CDS             12825..13670
                     /locus_tag="PC545_001331"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HDF2456108.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            13757..14932
                     /locus_tag="PC545_001332"
     CDS             13757..14932
                     /locus_tag="PC545_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2456109.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTTLIVKR"
     gene            15128..16030
                     /locus_tag="PC545_001333"
     CDS             15128..16030
                     /locus_tag="PC545_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2456110.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKIDEEKANFEPQDLNLQIIYDDFDIIMVNKPPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGYVDEDLINRQALHAYKLEFEQP
                     RTKEKLKFKADIPEDMKELISKLR"
     gene            16044..17048
                     /locus_tag="PC545_001334"
     CDS             16044..17048
                     /locus_tag="PC545_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2456111.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVSNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(17192..17419)
                     /gene="acpP"
                     /locus_tag="PC545_001335"
     CDS             complement(17192..17419)
                     /gene="acpP"
                     /locus_tag="PC545_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDF2456112.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            17591..18016
                     /locus_tag="PC545_001336"
     CDS             17591..18016
                     /locus_tag="PC545_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456113.1"
                     /translation="MKYVGLLLTSVGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            18064..18270
                     /locus_tag="PC545_001337"
     CDS             18064..18270
                     /locus_tag="PC545_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456114.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            18452..18631
                     /locus_tag="PC545_001338"
     CDS             18452..18631
                     /locus_tag="PC545_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456115.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            18665..18865
                     /locus_tag="PC545_001339"
     CDS             18665..18865
                     /locus_tag="PC545_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456116.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSCGGFFDGEVGCGC"
     gene            19118..20134
                     /gene="ccpA"
                     /locus_tag="PC545_001340"
     CDS             19118..20134
                     /gene="ccpA"
                     /locus_tag="PC545_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HDF2456117.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVAGF
                     NDVKIARMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVT
                     ERKK"
     gene            complement(20257..20655)
                     /locus_tag="PC545_001341"
     CDS             complement(20257..20655)
                     /locus_tag="PC545_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456118.1"
                     /translation="MKKLDTESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDNIMEKISDAENQRDTKPAKTKKAKPTKGKS
                     NSPAPKNTPGSQQKKSKSLFKKTKKSNPHE"
     gene            20867..21772
                     /locus_tag="PC545_001342"
     CDS             20867..21772
                     /locus_tag="PC545_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456119.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHGNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSRDERKILIKIESVL
                     DLVRGIKKLNNIDMVDTEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNDDEDNN
                     DELNYEDEELDCEDKELDDDELTDEGEMEDNEDELVEDKNTGYENIENDEDIVYDEEL
                     YNINEQESEYNYNEKNTKTRAKKKR"
     gene            21887..22067
                     /gene="ssrS"
                     /locus_tag="PC545_001343"
     ncRNA           21887..22067
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PC545_001343"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            22250..23467
                     /gene="cdeC"
                     /locus_tag="PC545_001344"
     CDS             22250..23467
                     /gene="cdeC"
                     /locus_tag="PC545_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HDF2456120.1"
                     /translation="MQDYKKNKRRMMNQPMSTMNEEEVYTDEINSEDMRGFKKSHHHN
                     GCNTDNKCECHDDCNPCNPCNPCKPNPCNPCKPNPCDDNCGCHDNCKCDCEPCEMDSD
                     ECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGEPLTFE
                     TEVVEVFGSVPSAGQASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGRNCETT
                     FEFAVCGERNSECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALAFPAVR
                     AGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILKLECNE
                     CCEPFYELIIPNDIDLVLCLQETVSTLISEQIVVLASPNPIQPRLVDTFSKVCDFSQC
                     GPNHGSGKPSCHR"
     gene            complement(23567..24889)
                     /locus_tag="PC545_001345"
     CDS             complement(23567..24889)
                     /locus_tag="PC545_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2456121.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALIISIV
                     ASFNSSFLSLKFLKDAKLNLFILAVCPAIVIITLSNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGIVSG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKSIR"
     gene            complement(24940..25734)
                     /locus_tag="PC545_001346"
     CDS             complement(24940..25734)
                     /locus_tag="PC545_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2456122.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQSKYKDKISIKKGIEFGVQTQLMDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLKQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            25844..27289
                     /locus_tag="PC545_001347"
     CDS             25844..27289
                     /locus_tag="PC545_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2456123.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESKDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINDKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            27318..27527
                     /locus_tag="PC545_001348"
     CDS             27318..27527
                     /locus_tag="PC545_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HDF2456124.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            27547..28941
                     /locus_tag="PC545_001349"
     CDS             27547..28941
                     /locus_tag="PC545_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDF2456125.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSSNYMEFLSKSKTERLSV
                     KEIIKLAKENNYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAIPLALHGIVILNDGTKVDIS
                     IGEEDSDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNMPLEDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            29181..29396
                     /locus_tag="PC545_001350"
     CDS             29181..29396
                     /locus_tag="PC545_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2456126.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNTTFLYKIAK"
     gene            complement(29476..30642)
                     /locus_tag="PC545_001351"
     CDS             complement(29476..30642)
                     /locus_tag="PC545_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2456127.1"
                     /translation="MKGGVRKRGKKWYYYFDAGIVDGKRKKVERVGGNTKKEAEKSLR
                     DAINEYENAGIVFDETNMSLSDYLNFWYKEYVLLNCKYNTQESYRNLIENHIEPRLGK
                     CKLKSINPAIIQEFLNNKSKETYTQNGEEKHYTKGVLKAIYVVLNAALKSAVYPYKLI
                     KENPVQYASIPKNVLKVKNESDNKTITLDEFNKILEIYPKNTNIYIPLLIGFHTGMRK
                     GEILGLCWDNVDLDNNIIKVRKNLIKRKVSEFELASPKTKTSIRDIKIGDTLSRILKE
                     EKLNQKKQKIKIGKWYKETEYDWVCRKKDGSFVNHNNIDAAIRTINKKLNINFNFHCL
                     RHTHATLLLENGANVKYIQQRLGHSQLSTTMDTYSHVTSKMESETIDILEGILQ"
     gene            complement(30780..30986)
                     /locus_tag="PC545_001352"
     CDS             complement(30780..30986)
                     /locus_tag="PC545_001352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456128.1"
                     /translation="MNLSFNKKKKFELSKEELELIENWLGNKYAGDMTIPAIVDFSLE
                     TDIEYEKIVVYLAEKVLESRDKKH"
     gene            complement(31045..31266)
                     /locus_tag="PC545_001353"
     CDS             complement(31045..31266)
                     /locus_tag="PC545_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456129.1"
                     /translation="MLKELRKKKKLTQIELAKRVGCHRSQISRLENNEDKDLTIPILI
                     ELEIALGLEEKYLVNYFADEYIKKRKLHK"
     gene            31550..31687
                     /locus_tag="PC545_001354"
     CDS             31550..31687
                     /locus_tag="PC545_001354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456130.1"
                     /translation="MNKTKYYDILKLNLLMKKLKELDKNKINEYKIKVKEIHKINKKE
                     E"
     gene            complement(31724..32119)
                     /locus_tag="PC545_001355"
     CDS             complement(31724..32119)
                     /locus_tag="PC545_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456131.1"
                     /translation="MNRIKELREEKGISLDKLSEDLHINKSTLSRIENGLREPKKSTI
                     EEYANYFDVSTDYLLGRTDVRNSLFINKNEKDYDAENFKTEKELIENMYLDEDMKEVF
                     NIFSELSPDAREKALKVAELFLLDEKNKK"
     gene            32298..32531
                     /locus_tag="PC545_001356"
     CDS             32298..32531
                     /locus_tag="PC545_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HDF2456132.1"
                     /translation="MYLNRLEGLMKENRHTQKNVADILGLSSYGFRLKLKGKNEFKAS
                     EIKKISKLYNVSADYFFSDEVAKIAIKEERGNK"
     gene            32531..33319
                     /locus_tag="PC545_001357"
     CDS             32531..33319
                     /locus_tag="PC545_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDF2456133.1"
                     /translation="MKNLTIIKQNNQFLVESREVAELIEKKHDNLLRDIRGYKKILED
                     SSNLKSQDFFIESTYINTQNKIQPCYLLTKKGCDMVANKMTGEKGIIFTAIYVTKFEE
                     MERELKEQQPKLPTTYKEALQQLLIEVEEKEQLQLENQEKDKVIQLQQPKVLFADSVA
                     SSDNSILIGELAKLLRQNGIDTGQNRLFDWLRNNGYLIKRKGEDYNTPTQKSVDLGVI
                     ETKEGTRVHPDGHTSITKTPKITGKGQIYFINKFKKNNQISMLG"
     gene            33376..33513
                     /locus_tag="PC545_001358"
     CDS             33376..33513
                     /locus_tag="PC545_001358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456134.1"
                     /translation="MSNKKKYTLSITEELYNKCKEKANQKGMSVNEYILLVISKNLKN
                     I"
     gene            complement(33525..33692)
                     /locus_tag="PC545_001359"
     CDS             complement(33525..33692)
                     /locus_tag="PC545_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HDF2456135.1"
                     /translation="MPSKLPRYTLRIDNNLLEKIKYIAESEGRSANKEIEQLIKKHVE
                     SYEKINGKINV"
     gene            33845..33991
                     /locus_tag="PC545_001360"
     CDS             33845..33991
                     /locus_tag="PC545_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456136.1"
                     /translation="MPDELNEELHRQSQRKGLSKNAFIINILWKEFEDLQDLKDEQEV
                     DKYE"
     gene            33984..34622
                     /locus_tag="PC545_001361"
     CDS             33984..34622
                     /locus_tag="PC545_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456137.1"
                     /translation="MNNLQIFEKMEFGQIRMAEIDNKPYFVGKDIAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPTIRQT
                     GQYQAQQNVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIKVIKSERPYKQLSYFEN"
     gene            34681..35061
                     /locus_tag="PC545_001362"
     CDS             34681..35061
                     /locus_tag="PC545_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456138.1"
                     /translation="MKNNTSDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSALENTEIKGVCNQMKIKKLALATGEVFENIELKEFENKGECLPSNMVKVI
                     DGKQELLINKEFILSLEVKRKTTF"
     gene            complement(35051..35203)
                     /locus_tag="PC545_001363"
     CDS             complement(35051..35203)
                     /locus_tag="PC545_001363"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456139.1"
                     /translation="MVIPIAKNTKQTSKTVASKASKILKDGRYSAASKSVAGSALSQT
                     KKGPKK"
     gene            35262..35459
                     /locus_tag="PC545_001364"
     CDS             35262..35459
                     /locus_tag="PC545_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456140.1"
                     /translation="MAINDNINKILKDRDLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKIADYLDITLDELVGR"
     gene            35489..35932
                     /locus_tag="PC545_001365"
     CDS             35489..35932
                     /locus_tag="PC545_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456141.1"
                     /translation="MSKLGNISNFNLDKQEDKSFNDLDNISICFSEGIRKVVEMNLNN
                     YKNKISKYLNETSKIELLEPKELTIVISKGYPDYLMSVEEASKRLKIDKVFGYELIKN
                     GLLKSVDIGATKVSSYELDDFITRNQGKNIKEMLREMKEIREGVI"
     gene            35933..36079
                     /locus_tag="PC545_001366"
     CDS             35933..36079
                     /locus_tag="PC545_001366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456142.1"
                     /translation="MNVRVLIAYVQFCNDKQIKASFEGLRKYNRDIELVNLYLPSKLK
                     IKGD"
     gene            36082..36399
                     /locus_tag="PC545_001367"
     CDS             36082..36399
                     /locus_tag="PC545_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456143.1"
                     /translation="MKSLTIVRNAVEQQLNRANLEINKNEELYTKLRKKEKRDVLDEI
                     ELSNALREKSVNERLKIFAESLLEIIDTQIEIKEYEESEDYKIFQLISEELERDRPID
                     VQI"
     gene            36481..36756
                     /locus_tag="PC545_001368"
     CDS             36481..36756
                     /locus_tag="PC545_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456144.1"
                     /translation="MKTKNEIIKDLEDRLFLLRFTTVDEVDWDVKFGQISALESCIDK
                     HRKGWTLEQFKEHLEKHKSENMYGDYIDGFMSVLRRNIKDMEGLENE"
     gene            36749..37651
                     /gene="bet"
                     /locus_tag="PC545_001369"
     CDS             36749..37651
                     /gene="bet"
                     /locus_tag="PC545_001369"
                     /inference="COORDINATES: protein motif:HMM:NF015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDF2456145.1"
                     /translation="MNNKALELATCTLESGQILDYMTVKNYLVSGNGNVTDQEVLMFI
                     ELCKAQKLNPFIKEAYLIKFGNSPANIVVGKDVFVKRANKNPNFEGMKAGIVTVNKNG
                     EVFEREGSLKLPQEELIGGWCEVSVRGMKFPIKSVVSLEEYSKSQATWKQMPCVMIRK
                     CAIVTALREAFPEDLQGLYDSAEIKTVPDKLSQKPIEIGKASPSQKQGILKLASMKGL
                     YDYENKKDTSKLEEFCESNGFDLKELKFEEVDELIELLSEYEPKDDFIDAEFEEVKED
                     EVEVREEAENIDCQISMEEAINVD"
     gene            38362..38460
                     /locus_tag="PC545_001370"
                     /pseudo
     CDS             38362..38460
                     /locus_tag="PC545_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            38523..38696
                     /locus_tag="PC545_001371"
     CDS             38523..38696
                     /locus_tag="PC545_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDF2456146.1"
                     /translation="MGRIYAQKSGSLNEQDRLELLRLLGKAGYTVKIGREKQNSKTTY
                     TYFVEYTEEQEEK"
     gene            38712..39119
                     /locus_tag="PC545_001372"
     CDS             38712..39119
                     /locus_tag="PC545_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2456147.1"
                     /translation="MNTITLVGRLVADAELKYLPNSGTPKITFSMAVDRRFKDKNGNK
                     ITDFIQCEQLGKHVENLVQYLVKGKPIYAVGELNIYNYKDENGCWESITKVNVNALEL
                     LSSKNDNNAKQEYVPPGLDPQGFQAIDDDDIPF"
     gene            39453..39710
                     /locus_tag="PC545_001373"
     CDS             39453..39710
                     /locus_tag="PC545_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456148.1"
                     /translation="MIIIRSQNKLDLMRVNRVEIDSRYVYAVFEGESNVREIGRYKSE
                     ERAIEVLNRIQEAIIAGTKFDIINKDGVRCNKEKVFEMPVE"
     gene            39724..40089
                     /locus_tag="PC545_001374"
     CDS             39724..40089
                     /locus_tag="PC545_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456149.1"
                     /translation="MLKVERYFSGSVADNIFEDDLTLRNYLALYCCIYGVNKNGELVF
                     PTRGKMLIEFNVDRDRKKRKKASKVKLINVKTGEEKIFDSIDSAACFLRLQSQAVYQT
                     IKKKTKTRSGWKAEYIEEE"
     gene            40207..40542
                     /locus_tag="PC545_001375"
     CDS             40207..40542
                     /locus_tag="PC545_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456150.1"
                     /translation="MNILASVILVIGSFIAGRVYEYRLNLNENDEADSKVLLDVFNEI
                     SVLREENKSLKEKLQEKELLFINRLIDFLHDKKICECCIYDCKIDDIEYDCEDGIKKW
                     LDSEELIFE"
BASE COUNT        16022 a         4462 c         7169 g        13047 t
ORIGIN      
        1 tcttttatat ttatacactt ctttttattt tgtattcaaa taaaactagt atgaaatgga
       61 caaaataaaa atataaatta gaagaaagga gatttttaag atgagcatac tagaaaataa
      121 ttttttatac aaattaaaaa acaacactat ctttttttgt atatatacat tgttatttat
      181 ttttatttac aaagcgtttc catacatagc tccatttttt ctaggtgcta taatagcact
      241 tatgattaat cctatatcac aaaagttaga aaataagttt catataaata aaggtatctc
      301 tactcttgtt ctaagttttc ttgcagtggc tatagtcagt acagttacta caataatagt
      361 aataaattca atgaaagaat taatgggatt tttaaataat atatctgcta atcctgagga
      421 tataagtaat actattatgt attttttaaa taaaataaat gactttatga aatcatttca
      481 agaaatagcc aattttgatg tagaacaatt agtaaacaaa ttcagtggtg aagtaatgca
      541 aattacaaaa aatcttctta ctagtatact tggattggct acttctattc catatattat
      601 aatatttata attaccttgt ttattgctac gtattttata gcaaaagacc ttgataagat
      661 tgaaaatagt ttttataata tgtttactgt tgatgttaga aagaaggtaa aaaacgtaaa
      721 gaaagaggca gggctttcac ttgttggata tataagggcc tatacaatac ttatggctat
      781 aacatttttt gcaatctggg gaagttttgc tttatttgga ttgaaatacg gtctaatagt
      841 agggtttgta ggagctttaa tggatttaat tccattccta ggtataataa caatatatct
      901 tccagtaatt gtttattatt ttcttataaa aaattatttt atagctataa gtatgactgt
      961 gatatttttt gttttgtctt tgattagaga aatacttgaa cctaaactag tatcagtaaa
     1021 tgttggacta aatccattag ctaccctagc agcgattttt attggaatcc aagtaaaagg
     1081 aataattgga gtaatttttt gtttgggttt ggtatgtatg catgatatac ttaagaaggt
     1141 ggatattttt taattataat aattgataga ttttttaaaa ataaaatttt attcacaggt
     1201 aaataaaaaa atattaatct tattaagaaa gagggtatat acgtgaaaca aaaagtagtg
     1261 gaaagatttt taaaatatgt atcatttgat acaacatcta attctcagtg tgagaactgt
     1321 ccatcttcag aaggtcaaag agttttagct aagtatatag ttgaagagct aaaaacaatg
     1381 ggtgttgatg atgtgagttt agatgaaaat agttatataa tggctacttt aaaaggaaat
     1441 actgatggtg tggatactat aggattcata tcacatttag atacaattga agatgttagt
     1501 ggaaaagata ttaaaccaaa aataatagaa aattatgacg gaaaagacat agttttaaat
     1561 gaagcgttaa atgttattac gtatgtaaag gattctccag agttggaaga atttaaaggt
     1621 gacgatttaa tagtcactga tggaacaact ctacttgggt ctgatgataa agctggtata
     1681 gctgagatag tgactgcaat agagtatcta ataaatcatc cagaaattaa acatggagat
     1741 ataaaaatag gatttacacc agatgaagaa attggtagag gtgctgattt atttgatgtt
     1801 gaaaaatttg gagcaaaata tgcatatact ttagatggag gaatagttgg agaacttgaa
     1861 tgtgaaaatt ttaatgctgc aaatgcaact attactatac atggtagaaa tgttcatcca
     1921 ggttcagcta aaaataaaat ggtaaatgct atacatatag ctgctgaaat atcagaaatg
     1981 ttcccagcag atgaaagacc agaaacaaca gaaggatatg aaggattttg gcatttaaat
     2041 tctataggtg gaaatgtcga gaatgtaagt atggcttata ttattagaga ccattgtaaa
     2101 gaaaaatttg aaaatagaaa atctattatg atagagaata tagaaaaaat aaataagaaa
     2161 tacgataata gagtggaatt ggatttaaaa gactcttatt acaatatgaa agaaaagatt
     2221 gagccagtta tgtttatagt tgatatagct aaagaagcta tggaggaatt aggtataaaa
     2281 ccacgtttag ttcctgtaag aggtggtact gatggagcaa gattatcttt taatggatta
     2341 ccttgtccaa atatatttac tggtggatta aacttccatg ggaaaaatga gtgtatacca
     2401 gttagttcta tggagaaagc tactaaacta attgtaagaa tagctgaaaa atatgctgaa
     2461 agagtttagt taaaaagtat ttaaaaatta agcatttaaa gttataatag ctatatcatt
     2521 ctttataagt caagctgatt ataagcttgt gttgtactta aaatggtata gctatttttg
     2581 taattatttt tataaaagat aattattttt agtttagggt atcaataaaa agtatgaata
     2641 tcaataggta ttaacattta aaagggggaa ttgtattgaa aattaaatct aaaaaaataa
     2701 tagcatttgc actttcactt gggattatgt tgccaagttg tgtatatgca gacaatatta
     2761 gtaaaaatca aaatgcacag gtagatatga acaattatct tcaaggtaga aaatctccaa
     2821 aggaggaaaa acttattggt gaagatagat ttgatacagc gataaaaata tctcaaagtg
     2881 gttggaataa tggttcagaa agggtgtttc tagttaactc aaattcattg ccagatgcat
     2941 tggcttcaac tcctttggca agtaaattgg atgcaccaat actgcttaca aataaaaata
     3001 gtataccata taatgtaaca gaggaaataa aaaggcttaa tccagcagaa gttattttaa
     3061 ttggaagtga aggagctatt tcatcaagtg taaaagaatc tcttgaagat atggaagtat
     3121 tagtcagtag aatcggtgga gcagatagag aagaaacatc tttacttctt actagacagc
     3181 tagatgatac tggtgattta ggtgtctcaa aagtggcagt ggttaatggg tataatggac
     3241 tggcagatgc aacttcaata tcaagcccag ctgcaagtga taatacagct ataatatata
     3301 caggtaagga tagcataaga agtgaggcaa agaattttat tacacaaaac tcaactaaga
     3361 catacatagt aggtggggag tacagtataa gtaaaaaact tgaaggacag ttagtaaact
     3421 cagaaagact agcagggaca gatagaaaag atactaatgc gaaagtttta gaaaagtttt
     3481 atggaaaatc aagtaaggtt aataatatgt attttgcaaa agatggaagt ggtagagaag
     3541 ctgatttagt tgatgggctt gctgctggag tattagcatc aaaaacaaag tcacctgtag
     3601 tacttgcaag tggaagttta agtagtgcac agaaaagttt tataaaaaaa gtaaaggctg
     3661 ataaatttgt acaagttggt ggaggtaaaa atagtaaacc atatgcagaa gcattagcct
     3721 tacagtaata ttgtgtaaat caaaattaat atataataat agcaagtaaa ccaagttgca
     3781 ctttaatata tataataaaa taagtaaact aagttatatt tcaatatagc atataataaa
     3841 acaagtaaac cagattctat ttaattagaa gtttaaggta ttgtacttaa acttctaatt
     3901 gagatagaaa taaaatggtt tacttgtctt tattatgaat ttaataaatt aaagcttatt
     3961 ttatttttat taaaaattta ctaatcattg atatttatta tatttccagt attgatgtca
     4021 attaagttta tatcaagttc aagctcatcc tcatcatcag tttttatgat atctataatt
     4081 gcgacagaat ggcttccatc ttttgggata gaagtacttc ctggatttat tactattaaa
     4141 ttatcatcca tgtatagttt ttttacatga gtatgtccta aaactaatat gtcaccaccc
     4201 attttctttg ctttgtcaat aatttcttct tctgattcta tataaccatg attcaatata
     4261 attctaattt caccaaattg gctcatgaca taaggactct gaataggatg ttctataacc
     4321 atctggtcaa catctgcatc acagttccct tttacaatca caatgttttc taatttattt
     4381 aatgtctcaa taaatttttt aggattatat ccttctggaa tatcatttct aggtccatga
     4441 tataagacat ctccaccatg taataaaaca tcacaatcag ataatacatt taacgctttt
     4501 tcaaagtaaa gtaagctacc atgagtatca ctcattattc ctattttcat attaaaaatc
     4561 ctcctaagta cagtctataa aaatttttca gatactctat gccaaaactc atagtttgac
     4621 attctaagta attttatttt tgcatcagat atactaactt ttatattttc gatttgtcta
     4681 aatctgtgct caactccatc aacgacaatc aatacagaat cttcaaatct atattctggt
     4741 gcaatcttta taacagattc atttgaacat attatacttg atgtaaagca cctatatgca
     4801 ttggtattta tcggatgtaa aggtgtaatc tgcataagtt caagatttat atcaacaata
     4861 ctacctccag cagaatagtt ataggctgtt gaacctgtgg aagtagaaat aatcatacca
     4921 tctccactaa aattttgtat atgtttatta tcaaggctta aatttaggtg tatagtccta
     4981 gacttgtctc ctcttataac aacctcattt acagcaagca tattagaacc actagtagtt
     5041 gtatatactt ccgcattaag aagtgacatt tcttgaataa tataatcttt ttttgtataa
     5101 gcttcaataa agctatcaat cttatctggt aaaatatcag gaaaaaaccc cagatgacct
     5161 gtatttattc ccattatggg aatctcagga aaatcgaaat ctctaactgt tcttaaaaaa
     5221 gagccatcac caccaattga tataataagc tctgtgtctg gataaatatc tgaatacact
     5281 tcaaagccag cattgataag ttttctagtt aagagttctt tagtctcaag ggacttattt
     5341 aattggtttg tatttatagt tataattctt ttcatatatt cacctcttca tactttgtat
     5401 tataacatag ttttataatt cttaatttat aaatttataa atgacctttg tagtgaatgt
     5461 gttaaaatat gtataggtgg gtataaaaaa atgcataaga ggtgaagatg tgtttagcat
     5521 aaggaatatc acaaaaaaat taggaaaatt caagttaaat aatataaact tagagttaaa
     5581 ggaaggggat attgttggga ttataggacc aaatggttcg ggaaaaacga ctttaataaa
     5641 aattataatg gggattattg atgcagatga aggtgaaatt gaactttgta atgaaactat
     5701 tgaaaattct ccaataagct ttaaaaacaa tattggtttt gtatatgatt ctttacaatt
     5761 ttatccgcat ttaaaggtta aggaatttag aaaaatagtt tctttatttt ataagaattt
     5821 tgatagagaa aggtttgatg aataccttaa taagtttgat atagaagaaa atatgcatat
     5881 tgaaaattta tctaaaggtc aatctgaaaa gcttatgtta tcaagtgcac ttagtcacaa
     5941 tgctaagtta ttgattttgg atgaaccaac tgctgggata gacccaattg taagaactga
     6001 aataatgcag tacttacaag attttgtgaa aaatggaagt agtagtgtaa taatttctac
     6061 tcataatact gataatttaa ttaaaattgc tgattatctt gtttttataa atagaggaaa
     6121 tcagattttt actgttaaga aagaattaat agaacaagaa tataagataa ttagggcaaa
     6181 taaagcagag ttagaagcaa ttaaagaaag catagttggt gtgaaagagt ataaatatta
     6241 taatgaggca cttgttaaag ttggagatag cttgaaagta aaaagcctat tgatagaaat
     6301 tgataagcat aaggtcaaaa atcctactat agaagaacta atgtattatt atgtaaatga
     6361 ggtgagatga tgttcaattt aataagaaaa gatttaataa tagggattag ttcagatggt
     6421 atccgtaatt taaagtacat attgctattt tttgtgtttt attttttcct taattcaatt
     6481 tcatattata cagtaagtat tgttgttagt tatttgatat ttataaatac gtttgagtgt
     6541 gattatgaaa atgattcaag gatttttata aggagtatgc cagttagtat agaagatata
     6601 gtttactcaa aatatttatt aggtgtagga ttaataattt ctgtaacaat aattgtgtcc
     6661 ttactaagta aattgacatc actagtgttc tttagaaata tggttttaaa tgatgtattt
     6721 ttttctgtaa atatattttt agcaattttg acgattttac tgcctttaat ttttaagttt
     6781 ggatatggaa aaatgaaagt ttgtggtttt atagtaagta ttttaattta ctttgtatat
     6841 ggaagtcttt tgaagatgat tagtatgatt gtatatcaag taaagcattt taattatagt
     6901 aaagttggag gagtatatct ttcaaattat gtaactgata tagctaatac taagtatata
     6961 aatttatata gtatgacatt tttaacaata ataattttta taatatcaat gtatttttct
     7021 attaagattt caaaaaagaa taagtttaat tattaaaatt ttgattaata ttatagcaca
     7081 atagcacaaa atataaggag actaaaaatg aagaagttta taaaagtttc attttttatt
     7141 ttggggattt ttacactatt catagctata aataaattag tatatagaga tatggtaagt
     7201 gtcaaagatt atgaaataaa tcatttagag gaaattgttt ttgattttga tagcaggaga
     7261 agtagagatg ttggtactat tgtaagattt aaaataagca ataatagtaa atatagttat
     7321 aaattaaaca gtgctaaaat gaagtttgaa aattgtgtgg aaaataaaaa gggaatgaat
     7381 aagtgttctg catatgtaaa tgtagattta tttaatgaaa aaaattctat aactgaaaga
     7441 actctgaaat atggaataaa accaaataat caaggttatg ttgattttgt aattccaaaa
     7501 ggactaaatt ttgattatag gtactttaat gaatctggta tgagtgtaga gtataaagga
     7561 gaatatattg tcaatattcc aatggcaaaa ggactttatc tagtagttgg aaaaaataat
     7621 gatacatgga atgctaatat catggaataa tttgaaaaat aaatcaatta tagatataat
     7681 taagaataat atgaaattta taaaagttca gactaggagg gatatttaat gtcaataagt
     7741 aatgtaagag agtattttaa acaatttggc aaggaagaca gtatattaga atttgaacaa
     7801 tctagtgcta ctgtggaact tgcagctgaa gcagctgggg ttattccagc aagaatagct
     7861 aagacactct catttaaaat aggagatgat gcaattttaa ttgtgacagc tggtgatgct
     7921 aagattgata ataagaagta taaggctgaa tttaattgta aggctaaaat gttgacacca
     7981 gaagaagtct tggagtttac tggacatgct attggtggag tctgtccatt tggtttaaaa
     8041 aattctataa aagtctattt agatgattca atgaaaagat ttgatacagt atttcctgca
     8101 tgtggcagta gtaactctgc aatagaactt acatgtgaag aaatggagaa gttttctaag
     8161 tgtgaaaaat gggtagatgt ttgtaagaat tggtaaaaaa atatagtaaa ctatagtagg
     8221 aattagtaaa aaactacctc tgtatacaat ctatacagag gtagtttttt tgtgtaaata
     8281 tttataaatc taagaggaaa atctaattta aactaataat ccaaaataaa gttaaaaaat
     8341 tctgattttt tttcgaaaca ataaaaaaat gacaaaaaag tggaaaaaat gtaataaata
     8401 ttgttaaaaa aataattaga aattattgaa aaattatcct ttgagtgata ctattataga
     8461 tgtaagatat ttaaaaataa aaaatagtca gataaaaata cgattattta gctctattaa
     8521 aataattttt atacgataaa aatcttctgt acatggctaa aaaacagatg tacagacaat
     8581 atttaatgtt taatataccc taatataaat atcgtaaaca aattatttta tctgtaacca
     8641 gtgtgaaaga aatcttattc taacttgaaa tttcacaaaa ttccaaaaag cgagattttt
     8701 tttaagtata gttgatttta aattaacaat ttatgattaa attaatgtca taattggtta
     8761 gtataatcac atataaatat agagcgtagt ttgattaagg ttataaattt ctcgacgtat
     8821 attcaaatac cttattatag atgttgccga gattttaatt aataaatgaa aataaaatat
     8881 ttataaaata ctgaggaggc tattatggat ttaaattcta aaaaatatca gatgcttaaa
     8941 gagctatatg taagcttcgc tgaaaatgaa gttaaacctt tagcaacaga acttgatgaa
     9001 gaagaaagat ttccttatga aacagtggaa aaaatggcaa aagcaggaat gatgggtata
     9061 ccatatccaa aagaatatgg tggagaaggt ggagacactg taggatatat aatggcagtt
     9121 gaagaattgt ctagagtttg tggtactaca ggagttatat tatcagctca tacatctctt
     9181 ggctcatggc ctatatatca atatggtaat gaagaacaaa aacaaaaatt cttaagacca
     9241 ctagcaagtg gagaaaaatt aggagcattt ggtcttactg agcctaatgc tggtacagat
     9301 gcgtctggcc aacaaacaac tgctgtttta gacggggatg aatacatact taatggctca
     9361 aaaatattta taacaaacgc aatagctggt gacatatatg tagtaatggc aatgactgat
     9421 aaatctaagg ggaacaaagg aatatcagca tttatagttg aaaaaggaac tcctgggttt
     9481 agctttggag ttaaagaaaa gaaaatgggt ataagaggtt cagctacgag tgaattaata
     9541 tttgaggatt gcagaatacc taaagaaaat ttacttggaa aagaaggtca aggatttaag
     9601 atagcaatgt ctactcttga tggtggtaga attggtatag ctgcacaagc tttaggttta
     9661 gcacaaggtg ctcttgatga aactgttaaa tatgtaaaag aaagagtaca atttggtaga
     9721 ccattatcaa aattccaaaa tacacaattc caattagctg atatggaagt taaggtacaa
     9781 gcggctagac accttgtata tcaagcagct ataaataaag acttaggaaa accttatgga
     9841 gtagaagcag caatggcaaa attatttgca gctgaaacag ctatggaagt tactacaaaa
     9901 gctgtacaac ttcatggagg atatggatac actcgtgact atccagtaga aagaatgatg
     9961 agagatgcta agataactga aatatatgaa ggaactagtg aagttcaaag aatggttatt
    10021 tcaggaaaat tattaaaata gataaatatt aaaatataca caataggtcg atattaaaat
    10081 agatatggag gaaggattta tgaatatagt cgtttgtata aaacaagttc cagatacaac
    10141 agaagttaaa ctagatccta atacaggtac tttaattaga gatggagtac caagtataat
    10201 aaaccctgat gataaagcag gtttagaaga agctataaaa ttaaaagaag aaatgggtgc
    10261 tcatgtaact gttataacaa tgggacctcc tcaagcagat atggctttaa aagaagcttt
    10321 agcaatgggt gcagatagag gtatattatt aacagataga gcatttgcag gtgctgatac
    10381 ttgggcaact tcatcagcat tagcaggagc attaaaaaat atagattttg atattataat
    10441 agctggaaga caggcgatag atggagatac tgcacaagtt ggacctcaaa tagctgaaca
    10501 tttaaatctt ccatcaataa catatgctga agaaataaaa actgaaggtg aatatgtatt
    10561 agtaaaaaga caatttgaag attgttgcca tgacttaaaa gttaaaatgc catgccttat
    10621 aacaactctt aaagatatga acacaccaag atacatgaaa gttggaagaa tatatgatgc
    10681 tttcgaaaat gatgtagtag aaacatggac tgtaaaagat atagaagttg acccttctaa
    10741 tttaggtctt aaaggttctc caactagtgt atttaaatca tttacaaaat cagttaaacc
    10801 agctggtaca atatacaatg aagatgcgaa aacatcagct ggaattatca tagataaatt
    10861 aaaagagaag tatatcatat aatttaaggg gggataaaga atgggtaacg ttttagtagt
    10921 aatagaacaa agagaaaatg taattcaaac tgtttcttta gaattactag gaaaggctac
    10981 agaaatagca aaagattatg atacaaaagt ttctgcatta cttttaggta gtaaggtaga
    11041 aggtttaata gatacattag cacactatgg tgcagatgag gtaatagtag tagatgatga
    11101 agctttagca gtgtatacaa ctgaaccata tacaaaagca gcttatgaag caataaaagc
    11161 agctgaccct atagttgtat tatttggtgc aacttcaata ggtagagatt tagcgcctag
    11221 agtttctgct agaatacata caggtcttac tgctgactgt acaggtcttg cagtagctga
    11281 agatacaaaa ttattattaa tgacaagacc tgcctttggt ggaaatataa tggcaacaat
    11341 agtttgtaaa gatttcagac ctcaaatgtc tacagttaga ccaggggtta tgaagaaaaa
    11401 tgaacctgat gaaactaaag aagctgtaat taaccgtttc aaggtagaat ttaatgatgc
    11461 tgataaatta gttcaagttg tacaagtaat aaaagaagct aaaaaacaag ttaaaataga
    11521 agatgctaag atattagttt ctgctggacg tggaatgggt ggaaaagaaa acttagacat
    11581 actttatgaa ttagctgaaa ttataggtgg agaagtttct ggctctcgtg ccactataga
    11641 tgcaggttgg ttagataaag caagacaagt tggtcaaact ggtaaaactg taagaccaga
    11701 cctttatata gcatgtggta tatctggagc aatacaacat atagctggta tggaagatgc
    11761 tgagtttata gttgctataa ataaaaatcc agaagctcca atatttaaat atgctgatgt
    11821 tggtatagtt ggagatgttc ataaagtgct tccagaactt atcagtcagt taagtgttgc
    11881 aaaagaaaaa ggtgaagttt tagctaacta atttcttggg gagggtatta tgagtacaag
    11941 tgatgttaaa gtttatgaga atgtagctgt tgaagtagat ggaaatatat gtacagtgaa
    12001 aatgaataga cctaaagccc ttaatgcaat aaattcaaag actttagaag aactttatga
    12061 agtatttgta gatattaata atgatgaaac tattgatgtt gtaatattga caggggaagg
    12121 aaaggcattt gtagctggag cagatattgc atacatgaaa gatttagatg ctgtagctgc
    12181 taaagatttt agtatcttag gagcaaaagc ttttggagaa atagaaaata gtaaaaaagt
    12241 agtgatagct gctgtaaacg gatttgcttt aggtggagga tgtgaacttg caatggcatg
    12301 tgatataaga attgcatctg ctaaagctaa atttggtcag ccagaagtaa ctcttggaat
    12361 aactccagga tatggaggaa ctcaaaggct tacaagattg gttggaatgg caaaagcaaa
    12421 agaattaatc tttacaggtc aagttataaa agctgatgaa gctgaaaaaa tagggctagt
    12481 aaatagagtc gttgagccag acattttaat agaagaagtt gagaaattag ctaagataat
    12541 agctaaaaat gctcagcttg cagttagata ctctaaagaa gcaatacaac ttggtgctca
    12601 aactgatata aatactggaa tagatataga atctaattta tttggtcttt gtttttcaac
    12661 taaagaccaa aaagaaggaa tgtcagcttt cgttgaaaag agagaagcta actttataaa
    12721 agggtaaaaa tctaattaaa aatatatttg ctgttaacta gattaaatag tattttaatc
    12781 tagttaacag aatataaata ctaaaataac aggaggttat aactatgaaa ttagctgtaa
    12841 taggtagtgg aactatggga agtggtattg tacaaacttt tgcaagttgt ggacatgatg
    12901 tatgtttaaa gagtagaact caaggtgcta tagataaatg tttagcttta ttagataaaa
    12961 atttaactaa gttagttact aagggaaaaa tggatgaagc tacaaaagca gaaatattaa
    13021 gtcatgttag ttcaactact aactatgaag atttaaaaga tatggattta ataatagaag
    13081 catctgtaga agacatgaat ataaagaaag atgttttcaa gttactagat gaattatgta
    13141 aagaagatac tatcttggca acaaatactt catcattatc tataacagaa atagcttctt
    13201 ctactaagag accagataaa gttataggaa tgcatttctt taatccagtt cctatgatga
    13261 aattagttga agttataagt ggtcagttaa catcaaaagt tacttttgat acagtatttg
    13321 aattatctaa gagtatcaat aaagtaccag tagatgtatc tgaatctcct ggatttgtag
    13381 taaatagaat acttatacct atgataaatg aagctgttgg tatatatgca gatggtgttg
    13441 caagtaaaga agaaatagat gaagctatga aattaggagc aaaccatcca atgggaccac
    13501 tagcattagg tgatttaatt ggattagatg ttgttttagc tataatgaac gttttatata
    13561 ctgagtttgg agatactaaa tatagacctc atccactttt agctaaaatg gttagagcta
    13621 atcaattagg aagaaaaact aagataggat tctatgatta taataaataa gaatttggga
    13681 attaaaagtt taaataaaat tgtttaaaaa acaatttcgg atatatgaaa aatctaattt
    13741 aatgggggta atgaatatga gagaagtagt aattgccagt gcagctagaa cagcagtagg
    13801 aagttttgga ggagcattta aatcagtttc agcggtagag ttaggggtaa cagcagctaa
    13861 agaagctata aaaagagcta acataactcc agatatgata gatgaatctc ttttaggggg
    13921 agtacttaca gcaggtcttg gacaaaatat agcaagacaa atagcattag gagcaggaat
    13981 accagtagaa aaaccagcta tgactataaa tatagtttgt ggttctggat taagatctgt
    14041 ttcaatggca tctcaactta tagcattagg tgatgctgat ataatgttag ttggtggagc
    14101 tgaaaacatg agtatgtctc cttatttagt accaagtgcg agatatggtg caagaatggg
    14161 tgatgctgct tttgttgatt caatgataaa agatggatta tcagacatat ttaataacta
    14221 tcacatgggt attactgctg aaaacatagc agagcaatgg aatataacta gagaagaaca
    14281 agatgaatta gctcttgcaa gtcaaaataa agctgaaaaa gctcaagctg aaggaaaatt
    14341 tgatgaagaa atagttcctg ttgttataaa aggaagaaaa ggtgacactg tagtagataa
    14401 agatgaatat attaagcctg gcactacaat ggagaaactt gctaagttaa gacctgcatt
    14461 taaaaaagat ggaacagtta ctgctggtaa tgcatcagga ataaatgatg gtgctgctat
    14521 gctagtagta atggctaaag aaaaagctga agaactagga atagagcctc ttgcaactat
    14581 agtttcttat ggaacagctg gtgttgaccc taaaataatg ggatatggac cagttccagc
    14641 aactaaaaaa gctttagaag ctgctaatat gactattgaa gatatagatt tagttgaagc
    14701 taatgaggca tttgctgccc aatctgtagc tgtaataaga gacttaaata tagatatgaa
    14761 caaagttaat gttaatggtg gagcaatagc tataggacat ccaataggat gctcaggagc
    14821 aagaatactt actacacttt tatatgaaat gaagagaaga gatgctaaaa ctggtcttgc
    14881 tacactttgt ataggtggag gaatgggaac tactttaata gttaagagat agttttagat
    14941 tataatattt acaaattaaa tatttaatta tgaatttaac ctgtaagaat aactatttgt
    15001 tattcttaca ggtttttata gttaaaaata attattatct attaaacaaa aatagtgtaa
    15061 aatatataat atcatgattt tgaagatata aaaaagtagg aagatataaa agaaagtagg
    15121 taaaagtttg tttaaaaaag agaatcaaag atataatctt atatcgtata caaatgaaga
    15181 agaaatgact ttaaaagaag ttttattgga caagctaaat ttctcagtta ggtcattatc
    15241 aaaaatgaag agggaaaaga gtgttttagt aaatggagta tataaaaaac cgagtttaaa
    15301 ggtgtatagt ggtgatttaa tagaagtaaa aatagacgag gaaaaagcaa attttgaacc
    15361 tcaagattta aatttacaga taatttatga tgattttgat ataattatgg tgaataaacc
    15421 tccgtttatg gtagttcacc caacaaaaag ccactatgat aaaacaatag caaatggtat
    15481 tagttactat atagataatc aaaaagaaaa tgtaaaaata agatttgtaa ataggttaga
    15541 tatgaatact tcagggttag ttatagtagc aaaaaatgct tatgcacatc atactttgtc
    15601 tacagcaatg agtgaaaata aagttgaaaa aaagtatata acagtagttg atggaattat
    15661 aaaagaaaat gagggaacta tagatgagcc aatttataga cctacagaag attcaataaa
    15721 gaggataata gatgaaagag gacagtcatc tgttactcac tataaagtaa tagaaagact
    15781 agaaaatgca actgtattag aagtaagctt agaaactggt agaacacatc aaataagagt
    15841 acatatggct catataggtc atggaataat aggtgatgag ttgtatggtt atgtagatga
    15901 agatctaata aataggcaag cacttcatgc atataagcta gagtttgaac aaccaagaac
    15961 aaaagaaaaa ttgaaattta aagcagatat accagaggat atgaaagagt taatatcgaa
    16021 attaagataa tggggtgata tatatgataa tacataaatt tataatacat gttttggaca
    16081 aaaatagtga tgttccaata ttaaatgact ttgaaggcaa agttaatcaa gaagtagatg
    16141 gttttttcca gaaagctata aaaagaattg caaaagatga ggatttaaga aaaggtgttt
    16201 ttaaagatta taatgacaat ttaattaaaa actgttgtga acaaataata tatgatgaaa
    16261 gtactttttt gaaaaattct aaagaaattg catcttactt gtttgatgtt atgaaaatta
    16321 atgctgcact agaatcttgt gatttggcaa tttgcttata cactattaaa gatgaaaaga
    16381 gtgtagcaat actgaaatta gattataaaa agttgtacac tcattcaatt gaatttgtgg
    16441 atgataaatt taatattcaa atggtttcaa atgaaattgg tatcccagag actttaagac
    16501 aaaagcaagg agctttgatt agtctaagtg gtataaatga tgagtttcat ctaaggcttt
    16561 tagataagga tgctgaaaaa gaagggtcag aatctaagtt tgtaacagaa tttttaaatg
    16621 ctaaaaaaat agatgatgat aagtacaaga ccaaggtatt taaaaataca gctgaaaatt
    16681 ggataaccaa tgctcttagt aatgatataa agcaagctga agatgtaaga agtatattaa
    16741 attatacact gaaggaaaaa catgaagttg atataaatga ctttgttgat aattcaatta
    16801 aagatgatga gttaaaggat agctttaaag agcatatgga agaaaaaggt cttgacgaaa
    16861 gttttagtat agataaaaaa tgggttgaga aaaagcttaa aaaaagaagt ataaaaacgg
    16921 ataatggttt tgacataaaa gggaatctaa ctgattttga agacccaatg aagtatactg
    16981 taaaacaaaa tcaagatgga actatagata taataataaa gaatgttacg ttttatgaag
    17041 aaaagtaatt tatatactta gtatataaat taaatataag agcgtgtatt ttatatattg
    17101 taaataaatg taatctagtc tgtgcgaact ctaatataat ttattatgta gaagtttaca
    17161 cagactattt atatttgtag acaaaacgat actatttgtt ttcttcgata tatttacata
    17221 tatcaccaat acatttaaag ttttcagctt cctcgtctgg tatttctatt ccaaattcat
    17281 cttcaagggc cattataatt tcaacagcat ctaatgaatc agcttctaag tcgtccatta
    17341 aagatgcttc catagttatc tcatctaaat tgtctacccc taattgttct gctattattt
    17401 cttttatttt ctcaaacatt ttaagcctcc taaagcagtt ttaattaatt ttaatgttag
    17461 tttaatataa tatcaagaat ttttcaatat ggaatctata aaaaataata aaattttata
    17521 aatgtagtaa taaagtatta tttgatgaat aaactttatt gataacaaat ataacttagg
    17581 aggaaatgct atgaaatatg taggactatt actaacttca gtaggaatgt ttttacttat
    17641 agcagtaaat ttttattata attcaataac tttagatatg caacgtatag aggattatgt
    17701 aatggagacc aatttaatcc ttgaagatgt agctgaaaag gagagttatg tatctaatga
    17761 aaaagaagat tacatatcaa ggttaatgca tgtaaagaaa ggaatagaaa attcaaaaac
    17821 atccttcttg atagaaagat ataaggagta caaaataaaa tcaatagaaa gtttaatcta
    17881 tactatatca gaggagaaaa aagattatct ggatgaagtt gatagataca ataaacttgg
    17941 tgaaaaagaa ataaataaac taatcaataa aaacttttta gaagtaactt atctgtctat
    18001 tactacatat atttaattaa agagacaaaa cttagtctct taatgattca aggaggatta
    18061 gttatgacaa aattagaaga caatatggaa cgtttctttg gaggatgcga aggatttttt
    18121 gggaataaaa tgttgataat aatagttata gtattcttac tattatgcac agacatttta
    18181 gaagacttat tatgcgatga caatatatgg atatgggtta tattaatagt attgttatta
    18241 tttaacttcg atgatggttg ttgttgctaa aaaatattga ttattaaata ggtaaaacca
    18301 gtaacttata caagttatgg ttttaccttt tttagttcaa attaaaaaat ttaaaaaaat
    18361 agtaaaaata cattagcaaa atggaaacca aatgcatagt atatgttagg agcgaaaagc
    18421 tcaacaaaat atcaacagga ggtaacgtat aatgttagat agattctttg gagaagaagg
    18481 atgttttggt ggaggaggat ggtggataat agtactattc ttcctattct tagcttttgg
    18541 agatagctgg gcagaatttg acataatggc atggatacca ttcctaatag ttttattaat
    18601 actttgctgc tgtggtggat gcttagaata agaacacagt tattaaaaaa aggagggctt
    18661 tgttatgtta gatagatttt ttggagaaga aggatgcttt ggtggaggag gatggtggat
    18721 aatagtacta ttcttcctat tcttagcttt tgaagattgc tggagagaca ttgacataat
    18781 gtcatggata cctttcttaa tattattgct aataacttgt tcatgtggtg gattctttga
    18841 tggtgaagta ggttgcggct gctaatactt aaatcattag aataaaaaaa tgctggtatt
    18901 ttataccagc atttttttat tttatttttt aatatgattt aaattaagaa tgaatattat
    18961 taaatttgtt gaatataggg tataatataa aatataatgt atttaaatgt acttgctttt
    19021 atggtaaaat aaaaacttat ataaatgtag ttaaaatagg aaccttcaaa aatttggttc
    19081 agttttagat aaatttagta aaataaggag gatgaagatg aaaggcaata taacgataaa
    19141 agatgttgct aaacaagcag gagtgtcaat atctactgta tctagagtta taaatgattc
    19201 aaaacctgta actgatgaag tcaaacaaaa agttttagag gttataaaag agactggata
    19261 tataccaaat ccacttgcta gaagcttagt aacaaagaag agtcaattaa taggggtaat
    19321 agttccagaa gtttcagatt cttttgttaa tgaggtgtta aatgggatag aagaggttgc
    19381 taaaatgtat gactatgata ttcttttagc gaatacatac tctgataagg aacaagaact
    19441 taagagtata aatctattga gagcaaaaca agtggaaggt atagttatga tttcatggat
    19501 agttgaacaa gaacatatca actatataca aaattgtgga ataccagcga catatataag
    19561 taaaactgct agaaattatg atatatatac agtaagtact agcaacgaag aagctacttt
    19621 tgatatgaca gagcatctta taaagaaagg tcatgaaaag atagctttta taatgactag
    19681 taaagatgat actgttttag aaatggaaag acttgctggt tatgagaaag cactttcaaa
    19741 taacaatata gaattagaca agagtttgat taagtatggt ggaactgatt atgagagtgg
    19801 atacaatagt atgaaagaac tattagatga tggaataata cctcatgcgg cttttgtaac
    19861 aggtgatgag gctgccatag gtgctataaa tgctatatgt gatgctggat ataaggttcc
    19921 agaagacata tcagttgcag gatttaatga tgttaagata gctagaatgt atagacctaa
    19981 acttactaca gtatatcaac ctctatacga tatgggagca gtagcaataa gaatggttat
    20041 aaaattaata aataaggaat taattgaaaa taagaaaata gagttacctt atagaattgt
    20101 tgatagagaa agtgttacag aaagaaaaaa ataaaactat taaaatcaat caggtagtgc
    20161 cttaggcact acctgatttt ttatgtcaat aatatcaaat ttaataatag attaactaaa
    20221 aataattatc taattgctta aatagtttat ttttatctat tcatgaggat tagacttttt
    20281 agttttctta aataaacttt tagatttttt ttgttgacta cctggtgtat tttttggtgc
    20341 aggtgaattt gacttacctt tagtaggttt agccttctta gttttagctg gtttagtatc
    20401 tctttggttt tctgcatctg agattttttc cattatatta tccagttttt tgcgttgatt
    20461 ttcatctaaa aatgctttga attttgatac tacttcatct ctgccatcta gattcttacc
    20521 tatttttata agttcctcca taagttcatt ttctgatttt cctttgtaac tatcagccat
    20581 cttttctatt ttatccttgt caatattttt ttgtttagcc attttttcta atgactctgt
    20641 atctaatttt ttcataaaaa aacccctttc tataaatata tattgaatta tattcatact
    20701 atttatatat agaaataata ttgaagtggt gaattgtgag tgatatataa tataaataaa
    20761 aattcttata gataatatat atgcactgag ctttaaaaat ggaacattaa aatatgaaaa
    20821 acatacttta atatagggca attatataat ataaggggga attgtcttga ataacttact
    20881 atttttgatt ggagtgattg cattgagtaa tggtaatatc tctatatttg atgataaaaa
    20941 taaatcaaaa ggaaataata gagttagaaa aaacaaaaaa aatgcaaatg ataaactttc
    21001 acagtctaca catggcaatt taaggtttga tgcaaatgat ataaaaagag gtttaaggct
    21061 catggattta agtgaagaag atttagaaat gggaatggaa ataataacaa gaactaaaaa
    21121 atacatgtca agagatgaaa gaaaaatact aataaaaata gaaagtgttt tagatttagt
    21181 tagaggaata aaaaaattaa ataatataga tatggtagat acagaagatg aaacggattt
    21241 tttcagaaaa atggatagtg atgataaaaa gaatatgatg ataaaggaaa ttatagatgt
    21301 attcccagaa aaaagaaaaa attcagttga aaaagctata ggaatgaaaa agaagataga
    21361 tttatttgca gaattatttt taccagatga ctttggtgaa ggtggattta gtttgagctc
    21421 tttggcaaat ataaacaatc ttggtagtat gaataatctc aaattgttag gaagtctttt
    21481 aagaggtgat gatagtaata atgatgatga agacaataat gatgagttaa attatgaaga
    21541 cgaagaatta gattgtgaag acaaagaatt ggatgatgat gagttaacag atgaaggtga
    21601 aatggaggat aatgaagatg aattagtaga agataagaac acaggctatg aaaatataga
    21661 aaatgatgaa gatatagtat acgatgagga actatacaac attaatgagc aagaatccga
    21721 atacaattat aatgaaaaaa atactaaaac tagagctaag aaaaaacgat aattaactaa
    21781 atacataaag aaaaaaggaa gaaaaagtaa tgatagaata agattatctc aattactata
    21841 taaaaccact ttaaaaaatt caaaatttat tatataataa taatatgaaa tcctgaaaca
    21901 tacgataagg cttttttaaa ttcaacctac aaatgaaata cgggagaccg tgtctggtaa
    21961 attataacat gcctagttag tttggacgtt aaaggttact ggcaagtcac ccacctggga
    22021 gtgcctaggg tgtgaattta cttagccacc ggtgtttcgg gacaaaaaaa gtgctactat
    22081 ttcaaatagt agtacttttt ttattaatat gcatttttct actaaatacg cctaattatt
    22141 gataaattca aaaagcaagt aacaattaag attataaaag catagtatta attaggcttc
    22201 aaaaagccaa agacttaata agaatcgctt acagggaggg caatgaaaaa tgcaagatta
    22261 taaaaaaaat aaaagaagaa tgatgaatca gccaatgtct acaatgaatg aagaagaagt
    22321 gtatacagat gaaataaatt cagaagacat gagaggtttt aaaaaatcac accatcataa
    22381 tggatgtaat actgataata agtgtgagtg ccatgatgat tgcaatccat gcaacccatg
    22441 taatccatgt aaacctaacc catgcaatcc atgcaaacct aatccatgtg atgacaattg
    22501 tggatgccat gacaattgta aatgtgattg tgaaccatgt gaaatggatt cagatgaatg
    22561 ttttgaaaac aaatgtggac cagaatgctg taatcctata tctccaagaa acttctctgt
    22621 atcaaatgca gtgccatttg caatagaggc taatagaata tttgatacta tgcaattcca
    22681 aacatttaca gatgcaacag gaccaaatgg agagccatta acttttgaaa cagaagtagt
    22741 agaagtattt ggttcagttc caagtgcagg tcaagcaagt gtaactatag aaaaaatatg
    22801 cttaagtaat gatggaatcg ttatagacac aggaatgaca actttagaag atttcgattt
    22861 agacccatta ggagatatag taggaagaaa ctgtgaaaca acttttgaat ttgcagtttg
    22921 tggagaaaga aactctgagt gctgtagaca aggaaaaggc aaatcagtag cttataaaca
    22981 aagaggatta actgtagcag ttcgtaattt agtactagag ctaagaggta gatgtggatg
    23041 tacagagttc gttgcattag ctttcccagc agttagagca ggaggtggat gtaagagaag
    23101 agttgattat gtagaattta cttttaacac actttcagca ccaatatgct tgccagctga
    23161 cggaagagct gttactttaa gacaagaata tcaaactaac ttaactgtag attgtatagg
    23221 aaaatctata ttaaaattag aatgcaacga atgttgtgaa cctttctatg aattaattat
    23281 accaaatgat atagatttag tactttgctt acaagaaaca gttagcacat taataagtga
    23341 acaaatagta gttttagcat caccaaatcc aatccaacca agacttgttg atactttctc
    23401 taaagtatgt gatttttcgc aatgtggacc taatcatgga agtggaaagc caagttgcca
    23461 cagatagata ggtagatata tagacagttt aaattataaa taaaaatcat ctaaggaagg
    23521 ctaccaaagt gtataattta cattggtagc cttcacgttt ttataactag cgtatagatt
    23581 ttagtgattt aaaaagcact atagtatgta atagacaagt aaagattgat actatcgtca
    23641 ttgtgtatat gtatgcataa atattaagtc ctggaattgg aagaaataca tataaagcta
    23701 ttagttgaat taacatgcca gtaatagtat ttatacttga agctaattct tttcttattg
    23761 aatgtaaaat gccagataga gtttgattta gggacataaa tagtggaact aaaaacatag
    23821 cttttagata ttctcctgca agtgtatttt taaatactaa tatacataaa tcttttccaa
    23881 agaaataaaa aaatattgat gatagtatgc ctacaaataa agtaagaagt atggagtatt
    23941 tgattttttt aattacactc ttacgctttc ttagagccat ttcttgtgaa atagttggta
    24001 ttaaatttac aataagcgct gaaccaacag taaaaggtaa aaatataaaa ggcattacca
    24061 tcccactgac aataccatac atacttaaag attgttgata agtcattcct gataatgcta
    24121 atcttgatgg tatcatcata gaacttatag attgcaaaag tatgtttgac attctattac
    24181 aagttatggg aatagacatt tttaatgttt ccatagaaga attataaaaa tcttttatgc
    24241 ttataatata cctattgtca aatgatgaat ctcgccataa gcaaataagc ataaatataa
    24301 tattactcaa ctcaccaatc gatataccta atagagctat ataacaattc atagatttat
    24361 tatttatgta cataacaagt aaaaaaacaa ataatatttt accaatttgt tcaatgattt
    24421 gtccaatagc agggattttt acattcttta tgccatagta atatcctctt agtacgtttg
    24481 aaagagttat tataactata gcaggacaaa cagctagtat aaatagattt aatttagcat
    24541 cttttaaaaa tttaagtgat aaaaaagaac tattgaaaga tgcaactata gatataatta
    24601 gtgctacgaa aaatgatata tataaagttg atataaataa agcattagta ttgtgtctat
    24661 cgtttaaagc ttttctcttt gcaactagac aactaagtgc agtaggtata cctgttgttg
    24721 taacagctaa gcaaatcatc aaaaagttaa aaatcatatg atagatacct aaacctgttt
    24781 cacctagtgc tcttgataag aatattttat ataaaaaacc aaatattctt acaataaaat
    24841 tggatataaa taaaataact gttgatagta ctaaataagg tactttcaaa aaaatcacct
    24901 ccttatcttt taaatatatt cacaaggagg tgtaaaaatt tataatttta taaattctgg
    24961 tttcaattta ttaaatttag atacatattt aaatcccata ttttttagta atgaatatat
    25021 gtagtcaaac tcacatgcaa cttgacttat atgatgtgaa tctgaacctg tggttatgat
    25081 ttcccctccc aagtctttgt acatttgaag tatatactta gatggggtta ggtctggaag
    25141 attatatcta taacaagagg tatttatctc gatacctttg ccatcataaa tagcttgttt
    25201 aagagtttct tcaattatat cagaaaatag tctatcatct aaaatagtgt cataaggtgc
    25261 atacctttta atcaaatcta aatgacctaa gacagagtaa tctttataat tcttaacaat
    25321 attatataaa taaagatagt aattttcata aacttcatgt tgagttttat ctttaaaata
    25381 attaccaagg tataagtcca ttgtatctat agcatggata gaacatatta taaagtccaa
    25441 tggatattga tgtgcttcct ttttgtatgt atccattaat tgagtttgaa ctccaaattc
    25501 aattcctttt tttattgata ttttatcttt atatttgctt tgaagagtct ctaaactctt
    25561 aaaataatct tcgtagacaa ttgaaaaaga atcatcagca tatacatcat agtcaacatg
    25621 gtctgtgaaa caaatttcct ccaaaccgag ttcaatagat ttttttacca tttcttccat
    25681 tgtagatttt ccgtctgtag agaaacttga gtgcatgtga tagtcgtaaa acataaatat
    25741 ttcccctttt tataaaaatt ttggtattat atatacatac taaataatat caaatataca
    25801 taaaagcatc aataaaatat aaatacaatg agcggtgata aatttggaaa aatacagaat
    25861 agatttaaat gagaaaaaat caactaaata tatacagata tttaatcata taaaagaatt
    25921 gataataaat aaagagttag tagaacatga aaaattgcct ccaataagaa aactatcaaa
    25981 ttttttacaa gtaaataaca ctactatagt taaggtatac gagcttttag aaaatgaagg
    26041 atatgtatat aaaattatag ggagtggtac ttttgtatca agtttaaatt cttataaaag
    26101 cggtatagat aacaggaaaa ataagattat tgtgcaaaga gaagatttaa ttcattttga
    26161 caatgggaat ccatctaatg atatgtttcc tatcgattca tttaaaaatg ctgtaaatat
    26221 ggcattatct aaagatggta gttcaatatt tgaatatgat gaaggtcttg gctctgaaga
    26281 attaagagaa aaaatggtag agtatctaag tgatggaaat ataaaaacta ctaaggaaaa
    26341 tattcaaata atatcaggtg ctcaacaagg tatagatata gtttgtaaag gacttataag
    26401 ttattcagat gttgttttta tggaagaacc ttcttacaat ggagcaattg aagtgtttaa
    26461 aagtagaggt gcaaaaataa tatctatacc tatgttagat gatggaattg atataggtat
    26521 tttaaaatta aagttggaaa aaataaaacc aagacttata tacattatgc ctaattttca
    26581 aaatccaact ggaatatctt actcaacata taaaaaaaag aaattgatag aattggcaga
    26641 agaacatgat ttttatataa tagaagatga ttttataagt gattttgttt ttgaatcaaa
    26701 ggacaataga acaattagaa gttatgatga taaaaataga gtagtatata taaaaagctt
    26761 ttcaaaaata cttatgccag gtcttaggat aggaattgta gaaatgccaa atgagttgtt
    26821 aaaaaaagtt ttatgggcta aatattcatc agatatatca acaccaggtt taattcaaaa
    26881 atcaatgtat tactatatga ataattttga ttggaagaat tatttgagtc atatagagaa
    26941 gatatatact gataagtata aattggcaaa aaacttgatt aatgacaaat tgagtggtaa
    27001 attaaaagtt agaaaatctt gtggaggaat aaactttttt ttagaattac caagaggata
    27061 tacatctcaa gcatttacaa attttatgtt aaacaaagga gtgtcaatgc ttccagggac
    27121 ttattttttt gacaatctag tggatgatag attctttagg ataaatatag ctcgtccaag
    27181 catggaagaa ctagaaaagg gaatatctat aataagtgat aatatagatg agtttttttg
    27241 tgaatataaa aacaagttag atataaaaag caataaatta ttttattaaa taaatagagg
    27301 ttttttagga ggaaaaaatg tttgacaaga agaaattaga taggattaat gaattagcaa
    27361 agaaaaataa agagggaatc ctttctgctg atgaaatcaa agaaagagaa attttaagga
    27421 aagaatattt agaaaacttt agagcccact ttagatcaag actagatagt gtcaaagtag
    27481 tttcaccaga agaatatgag caatacatga aaaacaataa aaattagaac ttagtaggag
    27541 gaaaatatga acttaaagta cgaatttaag aatgcttggg attttgaaag aaaagaaaat
    27601 actattgaac aaattatgca atattctagc aattatatgg agtttttaag taagtcaaag
    27661 actgaaagac tttcagtaaa agaaattata aaattggcta aagaaaataa ctatatttca
    27721 atagatgaag ctatggaaaa aggaagcata agttgtggtg ataaaattta tgttataaat
    27781 aaagagaagg cagtagcact ttttgtaata ggtaaaaatt atatagaaaa aggtatgaag
    27841 attataggaa gccatataga ttcgccaaga ttagatttaa aaccaaatcc actgtatcaa
    27901 gagagtaatc taggtttttt taagactcat tattatggtg gaataaaaaa ataccaatgg
    27961 acggctattc cacttgcttt acatggtata gttatattaa atgatggaac aaaagtggac
    28021 atatctattg gggaagaaga tagtgaccca gtattttgtg taactgattt attgattcat
    28081 ttggctggag accaaatgca aaagaaacta tcagaaggaa ttagtggaga agctttaaat
    28141 atattaattg ggaatatgcc tttagaagat gaagaaaaag aacctataac agcaaatata
    28201 ttaaagattt taaatgagaa atacaatata gttgaagaag atttattaag tgctgaaatt
    28261 gaagtagttc cagctggtaa ggctagagat ttagggcttg ataggtctat ggtgttggga
    28321 tatgggcatg atgatagggt atgttcatat gctgctgtaa aagctatact agaaactgaa
    28381 caaccagaat ttacttcagt tgcattatgt gtagataaag aagaaattgg ttctaaaggg
    28441 aatacaggta tgcactcaaa gttttttgaa aatactgtgg ctgaactgat tgctttagaa
    28501 ggagattatt gtgatattaa agtaagaaga gctttagcaa atagtaaggt attatcagct
    28561 gatgtttctg ctggatatga cccaaatttt ggagaagcat atgaaaagag aaattcggca
    28621 tatatgggaa atggagtagt tttaactaag tatacaggtt caagaggaaa gagtggatgt
    28681 aatgatgcta atgctgaatt tatgtcagaa gtaaagagaa tttttaataa aggaaatgta
    28741 gtatggcaaa ctgctgaact tggaaaagta gaccaaggag gaggcggaac tatagcccat
    28801 atattggcaa atcaaggtgc agaggttata gactgtggtg ttggagtttt aaatatgcat
    28861 gcaccacatg agattgtctc taaagtagac atatatgaaa tgtataaggg atataaggca
    28921 ttttttaata taaatttata agtaaaaata taaatgtaat aagtgaataa aggctgttgt
    28981 ataaacaatt taatctgttg tacaacagtc ttatttttat gtaaaaatag caatttgtca
    29041 cactagagaa aataaaagaa aatactgtca aattaaaaat tattttaaaa aaaattgaca
    29101 acgatttcat cttgttataa tatgtaataa agaacttgtt atataatgtt ataaaaatgt
    29161 tacataggga ggtgagcaaa atggatgaaa agagacaaaa aaaagaaatg gatttagaag
    29221 taaattcctt tgttccttta tatcaacaac tatatgacaa cataaaaaaa caaatagcat
    29281 ctggcatata taaaccagga gataaacttc catctgaagg agacttatgt aaagaattta
    29341 atataagtcg tataactgtg agaaatacga cttttttata taaaatagca aaataacagt
    29401 gtttgagcaa aaaaaaaaga atgtttttta tcgttttgcc acctgtttgc caccataact
    29461 tttttcggtg gcaaattatt gtaaaatgcc ctccaaaata tctattgttt cactttccat
    29521 tttacttgta acatgtgaat atgtatccat agtggttgat aattgactat gacctaatct
    29581 ttgttgtata tattttacat tagctccatt ttctaataat aatgtggcat gtgtatgtcg
    29641 caagcaatga aaattaaagt taatatttag tttcttgtta atagttctta tagcagcgtc
    29701 aatattgttg tgatttacaa atgagccatc tttttttcta caaacccaat catactcagt
    29761 ttctttatac cattttccaa ttttaatttt ttgttttttt tgattcaatt tttcctcttt
    29821 taatattcta gacaaagtat cacctatttt aatatctctt attgatgttt ttgtcttagg
    29881 tgatgctaat tcaaattctg aaacttttct ctttattaaa ttttttctaa ctttgattat
    29941 attattatct aaatcaacat tatcccaaca aagacctaat atttctcctt ttctcatgcc
    30001 tgtatgaaac cctataagta gaggaatata gatatttgta tttttaggat atatttctag
    30061 tattttattg aactcatcta gtgttatagt cttgttatct gactcatttt ttacctttaa
    30121 aacatttttt ggtatactgg catattgaac aggattttcc ttaatgagtt tgtaagggta
    30181 aacagcagat tttaaagcag catttaatac aacataaatc gcttttaaaa ctccttttgt
    30241 gtagtgtttt tcttctccgt tttgtgtgta tgtctctttt gatttattat ttaaaaattc
    30301 ttgaataata gctggattta tagattttag cttacattta ccaagtctag gttctatatg
    30361 attttcaatt aaatttctat aactttcctg agtattgtat ttgcaattaa gaagtacata
    30421 ctctttgtac caaaagttaa gatagtctga taaactcata tttgtttcat caaacactat
    30481 accagcattt tcatattcat ttattgcatc acggagcgat ttttcagctt ctttcttagt
    30541 gtttccacca actctttcta ccttttttct cttgccatct actatacctg catcaaagta
    30601 gtaataccac ttctttccac gttttcttac gccgcctttc ataaaatttc tcctctcaaa
    30661 agcttttttt atgtaattat ataataacat atatcaattt aaataaaata aataaaaaag
    30721 actataagaa acaaagtttt atgtttataa ttaatagtct tttttattta gatacatatt
    30781 taatgttttt tatcacgtga ttccagcact ttttctgcca aataaacaac tattttttca
    30841 tactcaatat cagtttctaa agaaaagtca acaatagcag gtatcgtcat atctcctgca
    30901 tatttattac ctaaccaatt ttcaattaat tccaattctt ctttgcttaa ttcaaatttt
    30961 ttctttttat taaaacttaa gttcaattta ttcacctcaa ataatattat atattaaaaa
    31021 tagtatttga tagaaacatt cgaattattt atgtaatttt ctttttttaa tatattcatc
    31081 agcaaaataa tttactaaat atttttcttc caatcctaaa gctatttcta attcaataag
    31141 aatagggata gttaaatctt tatcctcatt attttccaac ctagaaattt gacttctgtg
    31201 gcaaccaact ctttttgcta actctatttg tgttaatttc ttcttttttc gtaactcttt
    31261 taacatatat ttaacctacc ttttagataa ttttatatat gttagtttgt gtaatttctt
    31321 gtaataaatg tgcaatattc gcacactttt tgttggaaat ttgtgctaga atgtagttaa
    31381 gaaataactt tatctagata aagcaaaaat aatagaagct ataaaatata ataatatttt
    31441 acagttattt tatagtttta agctttgaaa aagaggtggt tgtaactatt tttaagaacg
    31501 tatgtttcgt ggaattaata aataaaatat tttatagggg atgatatatt tgaataagac
    31561 gaaatattat gatattttaa agttaaactt attaatgaaa aagttgaaag aattggataa
    31621 aaataaaatc aatgaatata aaataaaagt gaaagaaata cataaaatca ataaaaaaga
    31681 ggaatgattc ctctttttta ttatattcat tttcagaaat aaactatttt ttattttttt
    31741 cgtctagtaa aaataattct gcaactttta atgctttttc tcttgcgtct gggcttagct
    31801 cgctaaatat attaaaaact tctttcatat cttcgtcaag atacatattc tcaataagct
    31861 ccttttctgt tttaaaattt tcagcatcat aatctttttc gtttttattt ataaataagc
    31921 tatttctaac atcagttctt cctaataaat aatctgtaga cacatcaaaa tagtttgcat
    31981 attcttctat tgtacttttc ttaggttctc ttaaaccatt ttctatcctt gatagtgtag
    32041 atttgtttat atgtaaatcc tcgcttaatt tgtctagcga gatgcctttt tcttctctca
    32101 attcttttat cctattcaat ttgcacaacc cctttttata ttaattccaa aatagcaact
    32161 tttatttata ttatagcaac aaaattaatt attacaattt tttttgctaa aaaagcaaca
    32221 aaagtattga ctataatcac aaaacttgat attatataaa tatgaagttg ccaaaatagc
    32281 aaaaaggaag tgatttaatg tacttaaata gattagaagg attgatgaag gaaaatagac
    32341 acacccaaaa aaatgtggca gatatattag gtcttagctc atatggtttt agattaaaat
    32401 taaaaggaaa aaacgaattt aaagcaagtg aaataaaaaa gatatctaaa ttatataatg
    32461 tatctgcgga ttattttttt tcagatgaag ttgctaaaat agcaataaaa gaagaaaggg
    32521 gcaataaata atgaagaatc taaccataat caagcaaaat aatcaatttt tagttgaaag
    32581 tagagaagta gcagaattaa tagaaaagaa gcacgataat ttattaagag atataagagg
    32641 atacaagaag attttagagg actcatcaaa tttgaagagt caagatttct ttatagaaag
    32701 tacttatata aatactcaaa ataaaatcca accttgctac ttattaacta aaaagggttg
    32761 tgacatggta gctaacaaaa tgacaggaga aaaagggatt atatttacag cgatttatgt
    32821 aactaagttt gaagaaatgg agcgagagtt aaaagaacag caacctaaac taccaactac
    32881 gtacaaagaa gcattgcaac agttattaat agaagttgaa gaaaaagagc aattacaatt
    32941 agaaaatcaa gaaaaagata aggtaatcca gttacaacaa ccaaaagtac tgtttgctga
    33001 ttcggtagca tcttctgaca attcaatcct aattggagaa ttagcaaaat tgcttagaca
    33061 aaatggaatt gatacaggac aaaatagatt atttgactgg ttaagaaata atggttactt
    33121 aataaaacgt aaaggtgagg attacaatac accaactcaa aaaagtgtag atttaggagt
    33181 tatagaaaca aaagaaggta caagagtaca tccagatggt catacaagta ttactaaaac
    33241 acctaagatt actggtaagg gacaaatata ctttattaat aagtttaaaa agaacaatca
    33301 aatatcaatg ttaggttaaa gattaatagc actttgaaaa ctaaatacag aatattttga
    33361 aaaggaagtg attacatgag taataaaaag aaatatactt tgagtattac agaagaatta
    33421 tataataaat gcaaagaaaa agctaatcaa aaaggtatgt cagtaaatga gtacatacta
    33481 ttagtgatta gcaagaattt aaaaaatatt taatttttat taacttaaac attaattttg
    33541 ccatttattt tttcataact ttcaacatgt tttttaatta attgttctat ttctttgttg
    33601 gcagaacgac cttcactttc agctatatac ttgatttttt caagtagatt gttatctatt
    33661 ctaagtgtgt atctcggtaa ttttgatggc ataaatatag caccctcctt aagtctaaat
    33721 gatgtcacta ttataccacg taaattattt caaaaaaagt gttgacgcaa aggtgacgca
    33781 atgataatat aataaacaag gaggtggttc aaaagtgacg gcaaagaaga gagtaactgt
    33841 tagaatgcca gacgaattaa atgaagagct acatagacaa tctcaaagaa aaggattaag
    33901 taaaaatgct tttataataa atatcttatg gaaagaattt gaggatttac aggacttaaa
    33961 agatgaacag gaggttgata agtatgaata acttacaaat atttgaaaag atggagtttg
    34021 gacaaataag aatggcagag attgataata agccttactt tgtaggaaaa gatattgcaa
    34081 agtcattagg ttataaaaat accaatgatg caattttaag acattgcaaa ggggtagtga
    34141 aacacgaggg gtttaaaatt aatggtatta agattgcttt gataactgag ggtgatgttt
    34201 acagattaat agtaggttca aatcttccaa acgcagaaaa gtttgagagt tgggtatttg
    34261 atgaagtgct tccaactatt cgacaaactg gtcaatatca agcacaacaa aatgtaataa
    34321 cagaacttac aggaactata ggagatttaa aaggaacaat aaaagaatat aaaaagttgt
    34381 gtaagataac atgctctaag aaacaacagt attctaaata cataaagaat agactggata
    34441 tagacaaagc taataaagaa tacaaccaag tgaaagcaag gctattccta attctcggag
    34501 tggagaaatg ggaagatata gactttgaca catctaataa tttaatacaa attatagatg
    34561 attgtataaa ggttataaag tcagaaagac catacaagca gttgagttat tttgagaact
    34621 aaatacagaa tattttgaaa tatattgttt taattaatta ttaactagga ggttaaatac
    34681 atgaaaaata atacaagcga ttcaagagta aaatattttt gcaaatgtcc atactgcggg
    34741 tttgataatg aggtagaagt taaaaaaggg ttgaagccta agatatgttg catatgtaca
    34801 aaagaagttg agtatgaaaa actggagcaa caaagtgctt tggaaaatac cgaaattaaa
    34861 ggagtgtgta atcaaatgaa aattaaaaaa ttagcactag caacaggaga agtatttgaa
    34921 aacatagagc tcaaagaatt tgaaaataaa ggagaatgct taccatcaaa tatggtaaag
    34981 gttattgatg gtaagcagga acttctaata aataaagagt tcatattatc attagaagtc
    35041 aaaagaaaga ctacttttta ggtcctttct tagtttgtga cagagcacta ccagcaaccg
    35101 atttggaagc agcactatat cttccgtctt taagaatctt actagcctta gaagcaacag
    35161 ttttacttgt ttgtttagta tttttagcta taggtatcac cacctttaaa tgtatttata
    35221 ggatatatcc tacaaatata gtatatcaaa ggaggaaaat aatggcaatt aatgacaaca
    35281 taaataaaat tttaaaagat agagatttaa aagcatggaa attagcaaaa gaaataggtg
    35341 tagattcagg gaatttatat gcaattttaa gaggagaaaa taaaaatcca actatagata
    35401 cattaataaa aatagccgac tatttagaca ttacattaga tgaactagtt ggaagataaa
    35461 aattaaatac agaatatttt gaaaaggagt gagtaaattg ggcaatatat ctaatttcaa
    35521 tttagataaa caagaagata aaagttttaa tgacctagat aatatatcaa tttgtttttc
    35581 agaaggtatc cgtaaagttg tagaaatgaa tttaaacaac tataaaaata aaatctcaaa
    35641 gtacttaaat gaaacttcga aaatagaatt attagaacca aaagaactaa caattgttat
    35701 aagtaaaggt tatcccgatt atcttatgtc tgttgaagaa gcaagtaaaa gattgaaaat
    35761 agataaagta tttggatatg agttaataaa aaatgggctt ttgaagtcag ttgatatagg
    35821 ggcaactaaa gtttctagtt atgaattaga tgattttata actagaaatc aaggaaaaaa
    35881 catcaaagaa atgcttagag aaatgaaaga aattagagag ggggtgattt agttgaatgt
    35941 aagagtacta atagcttatg tacagttttg caatgataag caaataaaag caagttttga
    36001 aggtcttaga aaatacaaca gagatataga gttagttaac ttgtatttac caagtaaatt
    36061 aaaaattaag ggggattaat catgaaaagt ttaactatag taagaaatgc agtagagcaa
    36121 caactaaata gagctaattt agaaataaat aaaaatgagg aactttatac aaaacttaga
    36181 aaaaaagaaa aaagagatgt attagatgaa atagagttaa gtaatgcttt aagagaaaaa
    36241 agtgtaaatg aaagattaaa aatatttgct gagtcattac tagaaattat agatacacaa
    36301 atcgaaataa aagaatatga agaaagtgag gattacaaga tatttcaatt aatttcagaa
    36361 gaacttgaaa gagatagacc tatagatgtt cagatataag aaaagagcct agggtgaggc
    36421 tctaatcaaa aaacaaaaaa taaatttaat aattatatat tatagcataa gggggaataa
    36481 atgaaaacaa aaaatgaaat aattaaggat ttagaagata gattattttt attaagattt
    36541 acaacagtag atgaagtaga ttgggatgta aaatttggac aaatatcagc attagaatct
    36601 tgcatagata aacatagaaa aggatggact ttggaacaat tcaaagagca tttagaaaag
    36661 cacaaatcag aaaatatgta tggagattat atagatggct ttatgtcagt tttaagaaga
    36721 aatataaagg atatggaggg gttggaaaat gaataataaa gctttagaat tagcaacatg
    36781 tactttagaa tcgggtcaaa tacttgatta catgacagta aagaattact tagtaagtgg
    36841 aaatggaaat gtaacagacc aagaagtgtt aatgtttata gaattatgta aggctcaaaa
    36901 actaaatcca ttcataaaag aagcatattt gataaaattt ggtaattcac cagcaaatat
    36961 agtagttggt aaagatgtat ttgtaaaaag agcaaataaa aatcctaact ttgagggtat
    37021 gaaagcagga atagtaactg taaataaaaa tggagaagta tttgaaaggg agggcagttt
    37081 aaagctacca caagaagaat taatcggtgg atggtgtgaa gtatcagtta gaggaatgaa
    37141 gtttcctata aagtctgtag tgtctttaga agagtattca aagagccaag ctacttggaa
    37201 gcaaatgcct tgtgtgatga taagaaaatg tgctattgtt acagctttaa gagaagcatt
    37261 cccagaagat ttacaaggat tatatgatag tgcagaaata aagactgtac ccgataaatt
    37321 atctcaaaag cctattgaaa taggtaaggc aagtccatca caaaaacaag gaatacttaa
    37381 gttagcatca atgaaaggtt tatatgatta tgagaataaa aaagatacat caaagctaga
    37441 ggaattttgt gagagtaatg gatttgattt aaaagaacta aaatttgagg aagtagatga
    37501 attaattgaa ttactaagtg aatatgaacc aaaagatgat tttatagatg ctgagtttga
    37561 agaggtgaaa gaggatgaag ttgaagtaag agaagaagct gaaaatatag attgccaaat
    37621 aagtatggaa gaagctataa atgttgatta agtaggtgaa gcaccttgaa atatacaata
    37681 ttaggattta gtcagaaaaa agcagtagag ttagggttaa acacaaagga tttacttata
    37741 ttgagatggt ttgtagattt tctaggtagt ggtaggatgg caagaaaact tattaatggg
    37801 attgaatact attgggttga ttattcggga gttattaagg aactaccaat tctttataca
    37861 gagaaacacg atactattta tagagtactc aagaagctag ataaaatagg aatattagaa
    37921 catgctactt taaagcaagg tggtacatgg agttattaca aattaggata tagatacata
    37981 gagttgatta gtgatagtta tcaacaggtc ggaaataaat ccgactgctc ggaaataaat
    38041 ccgtaccctc cggatataaa tccgacccaa acggatataa atccggaacg aaaaacccat
    38101 ctactatatc cttctactat accttgtaac tatactacgc agtcgagtgc tgtagtgaat
    38161 aaaaataaaa aaattattga agaaaaaaca catttaaagc tgagtaataa tatgcaaaaa
    38221 aaagtatctg cttgggatga aaatagacta ttaaaagcag tagatatatt catagaaaaa
    38281 aagggagaag catttagttt actagaaaaa atttatagag acaatagaaa tttcatggat
    38341 aaggatgtag agcaggttaa taagaataaa tttagaaact ttgatgaaac ctttactcaa
    38401 tacacaactg atgaactaga cgaaattatt aaaaaaagtc aaaaagttaa attcaaataa
    38461 aattaaatta aacttctagg aagtaatata ttaatattta tttcctagaa aggaggatta
    38521 agatggggag aatatacgca cagaaaagtg gttctttaaa tgaacaagat agattggaat
    38581 tgttaagatt acttgggaaa gctggatata cagtaaagat tggtagagag aaacaaaata
    38641 gtaagacaac ttatacttac tttgttgagt atacagaaga gcaggaagaa aaatagaagg
    38701 gggctagtta aatgaataca ataactttag ttggaagatt agttgcagat gcagaattga
    38761 agtaccttcc aaattcgggt actccaaaaa taaccttttc aatggcagta gatagaaggt
    38821 ttaaagataa aaatggaaat aaaataactg attttattca atgtgagcaa ttaggaaaac
    38881 atgtagagaa tttagtgcaa tatcttgtta aaggtaaacc tatatatgct gttggagagt
    38941 taaatatata taattacaaa gatgaaaatg gttgctggga atctattact aaggttaatg
    39001 taaatgcttt agaactactt tctagtaaaa atgataataa tgctaaacaa gaatatgtac
    39061 caccaggact ggacccacaa ggttttcaag caatagatga tgacgatata cctttttaat
    39121 taagttaaat aatctaggga gtaattatgc aatattactt cctaggagtt aaaatattgg
    39181 agggatggaa aatggaagaa gagtatagaa aatttttttt agagcgtaat ggtgagaaaa
    39241 ttgagatggt acaaaattgc gaaggagcaa taaaaataag cttaaataat ataaaaacag
    39301 ataattcgca tagaccttgg aatttaaagg cagaaggaac agctacagtg acgctatatc
    39361 taggtaaact gctttttgaa gaaatgctgt ggcttaaaat attgtattta ttagaagtta
    39421 tagcagaaaa aatgatgatg ggggtattag gaatgataat aattagaagt caaaataaat
    39481 tagatttaat gagagttaat agagttgaaa tagatagtag atatgtatat gcagtgtttg
    39541 aaggtgaatc aaatgttaga gaaataggta ggtataaatc agaagaaaga gctattgaag
    39601 tattaaacag aatacaggag gctattattg caggaactaa gtttgacatt ataaataaag
    39661 acggggttag atgcaacaaa gaaaaagtgt ttgaaatgcc agttgaataa ggagggggct
    39721 gaaatgttaa aggttgaaag atattttagt ggttctgttg cagataacat atttgaagat
    39781 gatttgactc ttagaaatta tttagcacta tattgctgta tctatggagt aaacaaaaat
    39841 ggagaacttg tatttccaac acgtggaaaa atgctaatag agtttaatgt tgatagggat
    39901 agaaaaaaga gaaagaaagc ttcaaaagta aagttaatta atgtcaaaac tggtgaagaa
    39961 aaaatatttg attctataga tagtgcagca tgttttttaa gactacagag tcaggcagtt
    40021 taccaaacaa ttaaaaagaa aactaaaaca agaagtggct ggaaagctga atatattgag
    40081 gaggaataat ggaagtttca aggactgaat acacaattaa aagagcaaaa gagttatatg
    40141 ataatggaga ggatatattt attgctatag ataaggctag agaagaatat gaggagatgg
    40201 ttaaaagtga atatcttagc tagtgtgata ttagtaatag gaagttttat agctggtaga
    40261 gtttatgagt atagattgaa tttaaatgaa aatgatgaag ctgattcaaa agtactttta
    40321 gatgttttta atgaaattag tgtgttaaga gaagaaaata aaagtcttaa agaaaagtta
    40381 caagagaaag agttactatt catcaataga ttaatagatt ttttacatga taaaaagata
    40441 tgcgaatgtt gtatttatga ctgtaagatt gatgatattg aatatgattg tgaagatggt
    40501 attaaaaagt ggcttgatag cgaagaactt atatttgaat agaagaaata tctaattaaa
    40561 acagtttaga gagttgcaaa atatctttta gtataaatta ttgttgaagt gttttgtgac
    40621 tctcaaaaat gaaaataagg aggcgttgta ttgcttacat ttttagattt attcgcaggg
    40681 ataggtggtt ttaggctagg
//
LOCUS       DAMJVI010000024        40304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.024, whole genome shotgun sequence.
ACCESSION   DAMJVI010000024 DAMJVI010000000
VERSION     DAMJVI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.024"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            87..824
                     /gene="cas6"
                     /locus_tag="PC545_001376"
     CDS             87..824
                     /gene="cas6"
                     /locus_tag="PC545_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HDF2456151.1"
                     /translation="MSRISIFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGN
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            829..2304
                     /locus_tag="PC545_001377"
     CDS             829..2304
                     /locus_tag="PC545_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456152.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFCELLMDLKNELEKNDYKLKFT
                     CELNRNYLSLESAEYIDPKYISDYILENQLFKVFKNGKKETLEKIFSNIDNLTYVNFI
                     EELDKSSATEKQKEGIKKDFKNRRFPYVRNSGKYGLNTSLENMEKNVKRIVDIVIKSN
                     IEIDEIDKLDLKQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKR
                     SGQVESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKS
                     FYKKLLHEKISSIRLYRIDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREP
                     MNTRNLGKKMINNGNLSNLKELLLDNISVLRQLTGTSREMDFVSSSYNIGLYIKLCWI
                     VDGREEYKMKNYIESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQT
                     IMHVLVSNGILIGEGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2324..3340
                     /gene="cas7i"
                     /locus_tag="PC545_001378"
     CDS             2324..3340
                     /gene="cas7i"
                     /locus_tag="PC545_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HDF2456153.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYRDKVHA
                     KTSIATITYDLRRILHQEYGWNLSNIVFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKEKGSISKASPLRIIPFRSLNSYRGSTQLITNRGFLSSEFGRKYYDEREENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEEDGKLLLPEERKFMSKELREK
                     AVKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGV
                     IETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMW
                     YLKE"
     gene            3356..4153
                     /gene="cas5"
                     /locus_tag="PC545_001379"
     CDS             3356..4153
                     /gene="cas5"
                     /locus_tag="PC545_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HDF2456154.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLQGNYYDYY
                     KEHKAQNSILNYEVLKEVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPY
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4180..6588
                     /locus_tag="PC545_001380"
     CDS             4180..6588
                     /locus_tag="PC545_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDF2456155.1"
                     /translation="MKNIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSISKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINSILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIDD
                     SVLNYRKFKYILYKGMLNLIDHSASSRQKGIKFYNDFTDEEIDNMILNEIYKSQGNLE
                     KNNIEFNTIQKRLRELSGRNVLTVAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYR
                     KNIFQNKNDLDTRSSNDYIGLLHGDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCV
                     IATVDQLLLSMFKFPGYEKIFAAVKNASITVDEVHLLEPKMFLILIYFMQFACKYLDV
                     DFHLMTATLPKSYKEQMINKGIIFQEESNENVTDKGEIVFIESNKEEEICEGKDVKVS
                     FIKEKEIKSIVEDALENKQKILIIKNTIDSVNRTYEFLKENLSDKYGGVDIDVLHSRF
                     KFKDKKEKYSKILNGEGDIWISTQSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNN
                     KYEYGNFYILPSEDKIYDNKLKNTTKNILKDILKTNSIFTMGIRKTILDDYYDNSVVK
                     KYFEENFISCDKEIKNIYGINKEIFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSY
                     KIILEEDFYKEDRELQQDSIQVSGFIFNRLYYYGLISKVEGYMVLKSSSKFEYNSTVG
                     LILK"
     gene            7030..7977
                     /gene="prmA"
                     /locus_tag="PC545_001381"
     CDS             7030..7977
                     /gene="prmA"
                     /locus_tag="PC545_001381"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDF2456156.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQEGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKK"
     gene            7996..8754
                     /locus_tag="PC545_001382"
     CDS             7996..8754
                     /locus_tag="PC545_001382"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDF2456157.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRCKLGEKLEICD
                     KNNNEYICEIMNIDKSIVNLEILEKVDINRESELKVRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVVKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLSFKEAL
                     EDMKKNNVNICPYENERTVSIKHALKKCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            8756..10054
                     /gene="mtaB"
                     /locus_tag="PC545_001383"
     CDS             8756..10054
                     /gene="mtaB"
                     /locus_tag="PC545_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HDF2456158.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INQIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRELDVLFEQNIEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            10097..10447
                     /locus_tag="PC545_001384"
     CDS             10097..10447
                     /locus_tag="PC545_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HDF2456159.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDIPDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            10610..10789
                     /locus_tag="PC545_001385"
     CDS             10610..10789
                     /locus_tag="PC545_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDF2456160.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            10819..11262
                     /locus_tag="PC545_001386"
     CDS             10819..11262
                     /locus_tag="PC545_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDF2456161.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            11373..11870
                     /locus_tag="PC545_001387"
     CDS             11373..11870
                     /locus_tag="PC545_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDF2456162.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLIYLMWLVYAGFLNYFI
                     WFINK"
     gene            12063..12626
                     /gene="raiA"
                     /locus_tag="PC545_001388"
     CDS             12063..12626
                     /gene="raiA"
                     /locus_tag="PC545_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDF2456163.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENLDFIDNSSEFDDYDYDDEEDENIVIERRKKFNVKPMSSEEAILQMELVGHNFY
                     MFRNQDNFEINIVYKRKAGGYGLIEQD"
     gene            12839..13075
                     /locus_tag="PC545_001389"
     CDS             12839..13075
                     /locus_tag="PC545_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HDF2456164.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            13088..14431
                     /gene="yqfD"
                     /locus_tag="PC545_001390"
     CDS             13088..14431
                     /gene="yqfD"
                     /locus_tag="PC545_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HDF2456165.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRNELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEVPSNPEKRPLNDIRNEFDEDNKDNKDQNNSDENNSNQNSNNSQNNNSNNN"
     gene            14370..15485
                     /locus_tag="PC545_001391"
     CDS             14370..15485
                     /locus_tag="PC545_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDF2456166.1"
                     /translation="MKIIVIKIVIIVKIIIAITTNKIKLKILGGLIQLIIQKKFTIAD
                     GNFERELFGNFDENVKLIEKTLNIDVILREGNIILIGEEKNVDSALKLMNELHQTVSN
                     GKHLDKQSISYSLSLLLEGSEQKIKELEGTIVITQRGKAVQPKTLGQKEYIKLIENND
                     ITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRIILTRPAVEAGESLGFLPGDLKDKVDP
                     YLRPLYDALFEMLGADKFNKYLERGTIEVAPLAFMRGRTLDNSFIILDEAQNTTSEQM
                     KMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLIQATEILKGVPGIGSIMLTDRDVVRH
                     ELVQRIIRAYEKHDKREEFKKEERKKAKMQEKTFKRK"
     gene            15542..16003
                     /gene="ybeY"
                     /locus_tag="PC545_001392"
     CDS             15542..16003
                     /gene="ybeY"
                     /locus_tag="PC545_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDF2456167.1"
                     /translation="MDLILDDRQDKLEVSEELIEKIKDIIIECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            16009..16728
                     /locus_tag="PC545_001393"
     CDS             16009..16728
                     /locus_tag="PC545_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDF2456168.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            16847..17248
                     /locus_tag="PC545_001394"
     CDS             16847..17248
                     /locus_tag="PC545_001394"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDF2456169.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSGFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHSIKDLLPNYFSGNDLK"
     gene            17312..18205
                     /gene="era"
                     /locus_tag="PC545_001395"
     CDS             17312..18205
                     /gene="era"
                     /locus_tag="PC545_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDF2456170.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            18217..19596
                     /gene="mgtE"
                     /locus_tag="PC545_001396"
     CDS             18217..19596
                     /gene="mgtE"
                     /locus_tag="PC545_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDF2456171.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEEREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIIARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLREQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            19669..19824
                     /locus_tag="PC545_001397"
     CDS             19669..19824
                     /locus_tag="PC545_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HDF2456172.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            19790..20563
                     /gene="recO"
                     /locus_tag="PC545_001398"
     CDS             19790..20563
                     /gene="recO"
                     /locus_tag="PC545_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDF2456173.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTISLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            20556..21155
                     /locus_tag="PC545_001399"
     CDS             20556..21155
                     /locus_tag="PC545_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HDF2456174.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            21315..22193
                     /gene="glyQ"
                     /locus_tag="PC545_001400"
     CDS             21315..22193
                     /gene="glyQ"
                     /locus_tag="PC545_001400"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDF2456175.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            22193..24259
                     /locus_tag="PC545_001401"
     CDS             22193..24259
                     /locus_tag="PC545_001401"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDF2456176.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKEEYIFATIKQDGKETSEVLKDILPETIKSVVFPKAMRWGGKNLKFARPI
                     RWIVALLNDKVLEFDLEGIISSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIAGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKILIEHALD
                     NYSELDFNKEEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVKVNE
                     LLKDKKYGIALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLGICDL
                     SKIVYK"
     gene            complement(24425..25972)
                     /locus_tag="PC545_001402"
     CDS             complement(24425..25972)
                     /locus_tag="PC545_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HDF2456177.1"
                     /translation="MDNFKKILLNELPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSQYQLNKIYEIAIKHNLDKIHLTTRQAIQLHDLSINSIVDI
                     MEEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEVFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEPYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLEKFKEYYKLEKENGS
                     LELNIEEIDYSKPGVKIDIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLSTLLKELDN
                     VENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGIST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASSGNFYEWVNSNDNILNK
                     ITDKYKI"
     gene            26252..27019
                     /locus_tag="PC545_001403"
     CDS             26252..27019
                     /locus_tag="PC545_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDF2456178.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            27302..27499
                     /locus_tag="PC545_001404"
     CDS             27302..27499
                     /locus_tag="PC545_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2456179.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            27528..28604
                     /gene="vorB"
                     /locus_tag="PC545_001405"
     CDS             27528..28604
                     /gene="vorB"
                     /locus_tag="PC545_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2456180.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            28607..29326
                     /locus_tag="PC545_001406"
     CDS             28607..29326
                     /locus_tag="PC545_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2456181.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTIEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            29339..29878
                     /locus_tag="PC545_001407"
     CDS             29339..29878
                     /locus_tag="PC545_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2456182.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIKVPVTEIAQNLGNEKAANICMLGAMVKSLDLFTKEEFESGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            30035..31093
                     /gene="buk"
                     /locus_tag="PC545_001408"
     CDS             30035..31093
                     /gene="buk"
                     /locus_tag="PC545_001408"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2456183.1"
                     /translation="MKVLVINPGGTSTKISVFQDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFDDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKEGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVAEKVSYLAPIEIVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            31111..32004
                     /locus_tag="PC545_001409"
     CDS             31111..32004
                     /locus_tag="PC545_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HDF2456184.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIESISREIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMTNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPKCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            32034..33242
                     /locus_tag="PC545_001410"
     CDS             32034..33242
                     /locus_tag="PC545_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2456185.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEITQKVLNERGKFALAYGPTKGEDELLDLLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVNKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIRAEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            33266..34525
                     /locus_tag="PC545_001411"
     CDS             33266..34525
                     /locus_tag="PC545_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456186.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEREEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            35170..36633
                     /locus_tag="PC545_001412"
     CDS             35170..36633
                     /locus_tag="PC545_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2456187.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAISWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTALPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKHEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKEDKHELN"
     gene            36689..37825
                     /locus_tag="PC545_001413"
     CDS             36689..37825
                     /locus_tag="PC545_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2456188.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKISEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLVDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVDIFTKIMEEFGIYEDEQVMLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINSYAKDRVIDGIKILALTGFNLFENPELLKKMKEE"
     gene            38061..39176
                     /locus_tag="PC545_001414"
     CDS             38061..39176
                     /locus_tag="PC545_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDF2456189.1"
                     /translation="METLEVRNIELSGTNYEIGYRLGELVANMPEIIEGQINKSNLVS
                     KKEEKEMIELFDKYCPGLNEELQGFADAIQVNCNQILYYTMTYLKPGCSQVALAPELT
                     ENGHVLFARNFDFSHNMEDFVLCKTKVNGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSDGLRKPAIVGLQFWAVIRYLLENCKDVDEALEYLKDMPIAYNINLLLADK
                     SGNIALVETLDGKKEVNIINSLESKREYFLHSTNHTHIDKLHKLEPQSMKNSIHRYKL
                     IKEYINKSKKIGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNIGKFNILWGG
                     LENKWESYYLKNDIKSITQRININIEKAPSDFFDFIE"
     gene            39328..40197
                     /locus_tag="PC545_001415"
     CDS             39328..40197
                     /locus_tag="PC545_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2456190.1"
                     /translation="MDYLKYFKKAVMYIENNLHENITVNDVAKETGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVSSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGKKKKLELDFMRHLVGNITIKPVIKEIEKIKVIGIKDKVILENNSLPD
                     LWEKFRKVHHIVPNTLPSKRVFGICEAISEIHLVSESMEFNEIIGLEVNSYDVIPNSF
                     VSKTIKEGKYAIFTHTGSLDNLDKTYEYIWGTWFLSSKEELDIRDDFEVYDERFLGPS
                     NVNSQIDIYIPIK"
BASE COUNT        16175 a         4086 c         7455 g        12588 t
ORIGIN      
        1 taaaaaatta atagatatat atctattaat tttttatatt acaaatagag aataatgaat
       61 tatactagaa agtaggaggt gaaaatatga gcagaatttc aatttttatg gaatcatgta
      121 atggcaaatt tccaaatgaa aattcaatgt tgtcagtttc ctttataaaa aatattttaa
      181 atgcagaaaa caaagatttt gctaaaaaca tatttaacta tggggaagaa agaaaaaaca
      241 ataagcaaat aaaggatata aatacagcca tttacatacc aaatcttata agaagcaaaa
      301 atgagttact tgttgatgga gatataatat tcaatataag tttttataat tattctatgt
      361 tcagtaaatt atacaatgga attttaaaag taaaggagct agaatataaa ggttatagat
      421 ttaaaataaa aaatataaaa atacataaag aacacgagat aaaagaaaat ggtgttatat
      481 ttaaaactat gtctcctata atagtaaaga ataaagaagg taaatattta gatgtagaag
      541 attcaaatta tatagaatgt ttaaactata tagctaatct aactctggag agtataagag
      601 gtagtgggct tagaaagcca ttggaattta tacctcttaa cttcaaaaaa agagtgttaa
      661 aagaaaagat tcgtggattt aaagagagag aattttacta tataaatgct tatgcaggaa
      721 catttttttt aaaaggaaat atggaagatt taaatgcact gtataaaatg ggaattggat
      781 atagaaggac ggaaaatgca ggaatggttg atatattaaa gtagcgaggt gagagaaata
      841 aaagctaaat tttatagatc aggtgatatt gtaagagata ttggcattgt ttacttttgt
      901 gaattactaa tggatttaaa aaacgaacta gaaaaaaatg attataagct aaaatttact
      961 tgtgagttga atagaaatta tttatcttta gaatctgcag aatatataga ccctaagtat
     1021 atatctgatt atatattaga aaatcaactt tttaaggtat ttaaaaatgg gaaaaaggaa
     1081 accttagaaa aaatatttag taatatagat aatttaacat atgtaaattt tattgaagaa
     1141 ttggataaat cttctgcaac tgagaaacaa aaggaaggga ttaaaaaaga ttttaaaaac
     1201 agaagatttc catatgttag aaatagtggt aagtatggat taaacacgag tctagaaaat
     1261 atggaaaaaa atgtaaaaag aatagtcgat attgtgatta aatcaaatat agagatagat
     1321 gaaatagata aacttgattt gaaacaatat gaagaaaaag atagtgtatg taatgtttgc
     1381 aatataaata aaacaacaaa actagatatt gatttaagag aacgaaagga ctctaagtat
     1441 aactttttat ttagaggtgc agaaaaatca ggttttaaaa gaagtggaca agttgaaagt
     1501 aatatttgtt ttgagtgtga atttttcaac ttaatgtgtc ttttatatat aaatttaaaa
     1561 agaccaatgg tttttgcata tacgaatgat ttaagagaat tagcattttt aaatcataag
     1621 ataatgctta aaagaaaaat gtattcagat aaatcatttt ataaaaaact tttgcatgaa
     1681 aaaataagtt caattagact atataggatt gatattgata cgaataaagg tataattcta
     1741 aaatttgact ctattataga gtataaagaa ttattaaaaa aaattgaact tatagatatt
     1801 atagataatt ataatttttc aagagagcca atgaatacta gaaatttggg taaaaaaatg
     1861 attaataatg ggaatttatc aaatttaaaa gaattattac ttgataatat aagtgtttta
     1921 aggcaactaa ctggaacttc tcgagaaatg gattttgtat ctagttcata taatatcggt
     1981 ttatatataa aattgtgttg gattgtagat ggaagggagg agtataaaat gaaaaactat
     2041 atagaaagta atttaatata ttcaagagta ggtaaagact tatttaataa aatgacagat
     2101 gaatccagaa gaaaaaattt ttctatgaaa gtgattcaac tacttaagtc taatgataga
     2161 actcagctat ttcaaactat aatgcatgtg ttagtttcta atggaatctt aataggagaa
     2221 gggtttgtag aaggtctaat gcagagttct gaattagaat taaatacgaa tgtaggtcta
     2281 tttattcaag aaatcatgaa ataaaaaagg gggaaaattt aaaatgaatg aaaaaagagt
     2341 aaaaggagta acattaacta tactgacaga aagcccagtt ccactttcgt atgaccaagg
     2401 gtatggaaat tatacaccaa taaaaaaaga acaatataga gataaagtac atgcaaaaac
     2461 tagtatagct acaataacat atgatttaag aagaatactc catcaagaat atggttggaa
     2521 tttaagtaat attgtttttg gaaaaaaagg aaatatatat ccatctatta agaaaaatgt
     2581 aggatgtgta gatgaaaatg gattagagac tgatgtattt ggttatttaa taccacttaa
     2641 agagaaaggt tctataagta aggcttctcc gcttagaata atacctttta ggtcactaaa
     2701 ttcatataga ggttcaacac agttgataac aaatagaggt tttttaagta gcgaatttgg
     2761 tagaaaatat tatgatgaga gggaagaaaa tgaagtactt agagatgaaa actttccaac
     2821 aacacaagca ttagcaatag aagagacttt atcagattac tacgtttata caataacatt
     2881 ggagcttgat agaataggag tagtagaagt agaagaagat ggaaagttac ttttaccaga
     2941 agaaagaaaa tttatgagta aggagttaag agaaaaagca gtaaaagata tattagatgc
     3001 aattactgtg tttacaagaa acataaaaca ttcatcagta ctattaaaac cacttgcagt
     3061 aatgggtgga gcttttgata aagttgtacc atttttttgg gatgatgtag attataatgc
     3121 tgatagtggt gagataaatt tagagggagt catagaaact atagaaagtt atagccttaa
     3181 agaaagtaac acaatacttg ctatcaatga cagattaaaa atttctaata aaaaggaatt
     3241 aaataagttt aatttaagta aataccctgt aaaagagatt aaaaatctag cagatagatt
     3301 ggaaattgga gaagataata tgtggtattt aaaagagtag gaggtttttt gaattatgaa
     3361 agtattacaa tgtaaactta agcaaccaac tgctcactat agagacccaa aagtatttca
     3421 aaatgaatat ataagtactt taaatttacc atctaagacc acaataatgg gaatgataac
     3481 gtatttatgt gatagaagat taaactctga tatagatata ggaataattg gaacacatca
     3541 tcatagagaa ctagaatttt caagaggaga gaacattgat ttttggaatg aatatacaaa
     3601 tatgagaaaa ggcaaagata aagaaaagtt tttgcttcaa ggaaactact atgattacta
     3661 taaagaacat aaagctcaaa atagcatatt aaactatgaa gttcttaaag aagtagaact
     3721 gactatattt atttcttgca aggatgatga agaacttgaa tttattagaa aaaagcttga
     3781 aagtccatgt aaatatgcaa atttaggaag aaaagaagat tttgtaatac caagtgaaaa
     3841 agggtgtttt gtgaaagaag ttgagttaaa agaggttata cctatgaata ctcgtgatgc
     3901 tataaaagaa aatataaaat taaagaatac atatgtaaga gttgacttaa gagataaaga
     3961 gaatgtcgag actataataa atcaaggtgt attgattgca ttaccttata aatataaaga
     4021 tttagaagca aatagagatg atagagttta tgagttttgc cattatatat atgttgataa
     4081 tgatggtata tatcctaaaa atattaaggt aaatgtatgt attgatacaa aagaagtttt
     4141 tacatggctt taaattattt tgaaagatag gtgtttatca tgaaaaatat catgcaaaaa
     4201 gaaatattag ctaagtcata taatttaaga actaatcaac aagagcaaac attagttgaa
     4261 catattaagg atttatttga agtattagaa tctatattag aactaaatct atattcagat
     4321 aaagatgtag agatactaaa aatatgttgt gctttgcacg atttaggaaa aattaattca
     4381 attatgcaac aaaaaatgga gattaataat aaaatatcgt atagttgctc agaagaagag
     4441 aggaaaaagt tagagtctga caaaaaatct ttaaaaaaga tagcaagaca taatatattt
     4501 tctggagcat ttttaaaaga tatattagaa aaaatgaact tgtcagaaga agataagtta
     4561 tatatatata aaagtataat gttacatcat ggaaactatg aagattatat gaggttaagt
     4621 attagtaaag ttcaagaaga aatttatgag tatattgaaa aaggcatttt agaaaaagaa
     4681 gagtttaatc tgaaagatat agaaagttat ataaatagca ttttaaatgt agactttaaa
     4741 tttggtgaag atgtattgga ttatgattac atagataaat taagtgaaag tatgtttata
     4801 gacagtgatt ataacaatgg tcaaatagat gattcagttt taaattatag aaagtttaag
     4861 tatattttat ataaagggat gctgaactta atagaccata gtgcatcaag tagacagaaa
     4921 ggtataaaat tttataacga ttttactgat gaagaaattg ataatatgat tttaaatgag
     4981 atatataaga gtcagggtaa tttggaaaaa aataatatag agttcaatac tatacaaaaa
     5041 aggttgagag aactttctgg aagaaatgtt ctgactgttg catttacagg ttcaggaaaa
     5101 acagctgcag attatagaaa gacttttaaa agaaaattct ttttagtgcc aaataaaata
     5161 tcagcagaaa gtttttatag aaaaaatatt tttcaaaata aaaatgattt agatacaaga
     5221 agtagtaatg attacattgg attacttcat ggagatataa acttatattc agagaatgaa
     5281 gataatggag aacatgattt tgtattgaca ttaagggata tagatttgag tataaacttt
     5341 tgtaaacctt gtgtaattgc aacagttgac cagttattac ttagtatgtt taaatttccg
     5401 ggatatgaaa aaatattcgc agccgtaaaa aatgcatcta taactgttga tgaagtacat
     5461 ttattggaac caaagatgtt tttgattttg atatatttta tgcagtttgc atgcaagtac
     5521 ttagatgttg attttcactt aatgacagct acacttccaa aatcatataa agagcaaatg
     5581 ataaacaagg gaattatttt tcaagaagaa agtaatgaaa atgttactga taaaggagaa
     5641 atagttttta ttgagagcaa caaggaagaa gagatttgtg aaggtaaaga tgtaaaggtt
     5701 tcttttataa aggaaaaaga aatcaagtca atagttgaag atgctttaga aaataaacaa
     5761 aaaattctta taataaagaa tactattgat tcagtcaata gaacatatga gtttttaaaa
     5821 gaaaatttat ctgataagta tggtggtgta gatatagatg ttttacatag tagatttaag
     5881 ttcaaagata aaaaagagaa atatagtaaa atattaaatg gagagggtga tatttggata
     5941 tcaactcaat cagtagaaat atccttggat ttggatttca atataataat aagtgattta
     6001 gcaactatgg atagcttgat acaaagaatg ggtagatgta atagaaataa taaatatgaa
     6061 tatggaaact tttatattct acctagtgaa gataaaattt atgataacaa acttaagaac
     6121 acaactaaaa acatcttaaa ggacatatta aaaactaact ctatttttac aatgggaatt
     6181 agaaaaacta ttttggatga ttactatgat aatagtgtag tcaaaaaata ttttgaagag
     6241 aattttataa gttgtgataa agaaattaag aatatttatg gtataaataa agaaattttt
     6301 gatggattgg atttaatttt taattttgaa ccttacaaaa atattgttga tagtaagaag
     6361 gaagcagcta aaatttttag agatgttgat gttagttaca aaattattct ggaagaagat
     6421 ttttataagg aagatagaga gttacaacaa gattcgattc aagtatcagg gtttatattt
     6481 aatagattat attattatgg gttaataagc aaagtagaag gatacatggt actaaaatct
     6541 agtagtaagt ttgagtataa tagtactgta ggcttaattt tgaagtgagc atatgtttaa
     6601 atataatata cctttagaca ttgaaaatac aaaaagtact actgtaagct cactgtaaaa
     6661 actatttttt taaaaggaaa aataagttgt gagatagagt gtttacaagt gtatatagat
     6721 aataaatttg aggtttaaga ttaactaagt gggtttcagg tacatcgcga aaataattat
     6781 aagttggaga ttttgtatgt ttaagattaa ctaagtggat ttcaaatatt gatatagtag
     6841 atataataga agttttatta ttaggtttaa aattaactaa tcggattttt aaatattctg
     6901 actattatat aaggatatta ttagcttgaa tttaaaacca ataatataaa acaacaaaat
     6961 attatattta tgatataata aaagttatgg aaaaacagga atattactat agagaagaga
     7021 ggtaaactta tgaataattg gatagaagta actataaaaa caacaacaga agcagttgaa
     7081 cctataacga atatactata tgaacaagga gctggaggag ctgtaataga agacccaaaa
     7141 gactttttat tccaaaagaa aaatgaacta gactgggatt atgttgaaga agaagtattt
     7201 aaaaagaatg aagaagatga tgttcttata aaaacttatg tttctgaaga aaaaaatgtg
     7261 atggaatttg tcgaaataat aaagcaaaaa gttttagggt taaaagattt tggtatagat
     7321 ataggtgaag gaagtgtatc attggaccaa gtaaatgaag ctgattgggc aaatgcatgg
     7381 aaagcatact acaaaccaac aaaagtagga caaagggtag tagtaaagcc tacatgggaa
     7441 gattatgcta tgcaagaggg ggatttaatc atagagcttg accctggaat ggcatttggt
     7501 acaggtacac atgagactac tagtatgtgc attagagaat tagaaaagta cgtgaataaa
     7561 gattctaaag tatttgatat tggctgtggt agtggaatcc ttgcaatagc agcagctaaa
     7621 ttaggagcaa aagaagttgt agctgtagat ttagatgaag tggctgtaaa agttgcaaaa
     7681 gaaaatgttc ttgaaaataa agttgaaaaa agtgtatctg taatgcatgg taatttaact
     7741 gatgtaataa aagataaagc ggatgtaata gttgcaaata taatagctga tataataaag
     7801 atacttgcaa aagatgttca aaactttatg aaagaagatg ctatattcat atcttcaggt
     7861 ataattttgg ataaagtaga agaagttaaa gaaagcttaa ttgaaaatgg atttgaaata
     7921 gttgaagttc aaaaattagg tgaatggtca gcaatagttt ctaagcttaa aaaataaggg
     7981 atatggtgac gtataatgga tagatttttt gttgaaaaaa ataatataaa tctacaagat
     8041 aaaacttgca ctatagaagg tgaagatgta aaacatattt caaaagtttt aagatgcaaa
     8101 ttaggtgaga aacttgaaat atgtgacaag aataataatg agtatatttg tgagataatg
     8161 aatatagata agagtatagt taatttagaa attttagaaa aagttgatat aaatagagaa
     8221 tcagaactta aagtaaggct ataccaggga cttccaaaag ctccaaaaat ggaaatgata
     8281 cttcaaaaat taactgaagt gggtgtagaa gaaataatat tagttcaaac taagagaagt
     8341 gttgttaaag ttgatgacaa aaaagaagat aaaaaatttg aacgttggga aagaataatt
     8401 tatgaagcag caaagcaaag caaaagaggt aagataccaa aattaagagg tgttttaagt
     8461 tttaaagaag ctttagaaga tatgaaaaaa aataatgtaa atatttgccc ttatgaaaat
     8521 gaaagaacag tttcaataaa acatgcacta aaaaaatgtg attctaatat agatagtgtt
     8581 ggaattttta taggtccaga aggtggattt tctgaagaag aaatagaaca aatacaaaaa
     8641 aataattgta atgttgtatc attggggcct agaatattaa gaactgagac agcttctgtt
     8701 gtagcatcaa caatagcttt gtatgaacta agtgatttag gaggagaaaa ataaattgaa
     8761 aaaagtagct ttttatacac ttgggtgtaa agttaatcaa tatgaaacag aagctatgct
     8821 agaactattt gagaaagatg gttatgaaca agtcaatagt gaagagtatg ctgatgttta
     8881 tgttataaat acatgtacag ttactcatat gagtgataga aaatctcgtc aatatataag
     8941 acgagttaaa aagaagaatc cagatgcaat aatagccgtt gtaggatgtt attctcaggt
     9001 atctccagaa gaaattttgg atatagaaga agtaaattta gtcatgggca caaatgatag
     9061 aagaaagatt gttgaagaaa ttaaaaagat aaattcaagt aagaaagtta gtacagttga
     9121 tgacataatg aaagttaaag cttttgaaga aatagaaatc agccaaacta atggaaagac
     9181 tagagcattt atgaaaatac aagatggatg tgacagattt tgtacttatt gtatcattcc
     9241 ttatgcccga ggacgcgtta gaagtagaga tattgatagt atagtcgatg aagtgaaaaa
     9301 attggctaat aatggctata aagaggttgt attaacagga atacatgttg cctcttatgg
     9361 aaaagattta aaggataggg atattaaact tcttgatgta ataaaacaaa taaaccagat
     9421 agaaaaaata gaaagaatta ggcttagttc tgttgaacca attttattta cagatgaatt
     9481 tgtaaatgaa gttttaaaga tggataaagt ttgtcctcat taccatttat cacttcaaag
     9541 tggatgtgat gaaactctaa aaagaatgaa tagacgatac acaactttag agtacaaaac
     9601 tattgtagat agattgagaa gtaaaatgcc agatgtggca ataactacag atgtaatagt
     9661 aggttttcct ggagagacaa atgaagagtt taaaaaaacg tacgagtttt taaaagaaat
     9721 agagctttca caaatgcata tttttaaata ttcaccaaga aaaggaactc cagctgcaac
     9781 tatggaaaat caagttgacc ctcaaatgaa acactttaga agtgaacaac ttttaaattt
     9841 aagtaaagta aattttaata aatttgcaac taaatttatc ggccgtgagt tggatgtttt
     9901 atttgaacaa aatatagaag gcaataaata tgagggatta acttcaaatt atataagagt
     9961 tgttgttgaa agtgacaaaa atatacaagg tcagatatta aaagtgaaaa taaatgatgt
    10021 aaaagatgaa tatgtagaag gaattttgct ataacatgta gaaattataa tatttataag
    10081 ataggaggtg gaaagaatgg attgtatttt ttgtaaaata gcgaacggag aaataccttc
    10141 aactaaagtt tatgaagatg atagagtctt agcttttaat gacttgaacc cagttgctcc
    10201 atatcatata ttagttgtac ctaaaaaaca ttatgatagt ttaattgata ttcctgataa
    10261 agagatggac attgtgtcac atattcatgt tgttattaat aaaatagcta aagagaaagg
    10321 ttttgaccaa actggattca gagtcataaa taattgtgga agtgatggag ggcaagaagt
    10381 taaacatttg cactatcata ttttagctgg taaaaagctt cctaattacg aagcgggaca
    10441 aaactaaact aaattattga aaaattgagt tccttcttga taaaaatact tgataaaagt
    10501 aaaaatttag tttataataa acaaagtgtt aggttgtaat gcatattatg caaaaagaac
    10561 atagttacag tcccagcata attgcgctat tggagggagg gaaaaagaaa tgtcagaagt
    10621 aagagttaga gagaatgaaa cattagacag cgctttaaga agattcaagc gtcaatgtgc
    10681 aatgtctggc atcatgtcag aagtgagaaa aagagagcac tatgataagc caagcgttaa
    10741 gcgtaagaaa aaagctgaag cagcaagaag aaaaaacgct aagaaatagt agtaaataag
    10801 aataaagagg tgaaggggat gtcccttaag caaaagttac aagaggattt gaaatcttcg
    10861 atgaaaaaca aagatacagt gagaaaatct gtagtaactt taataagagc ttctataaag
    10921 cagtatgagg ttgataatag ggtcgagctt gatgaagatg gaattatcga tgtaatagca
    10981 aaacaattaa agcaacgtag agatgcatta gttgaatttg aaaaagctgg aagagaagac
    11041 ttaataaaag aaacagaagg tgaaattgaa gttttaaaag agtacctacc tcaacaatta
    11101 agcgaagaag agttagaaga aattgtaaaa tctactatat ctgaagtagg ggcaacttct
    11161 atgaaggata tgggaaaaat aatgtcagtt atccaaccaa aagttaaagg aagagctgac
    11221 ggaaaactta taaataagtt ggtaaaacaa aacttacaat agattgatat agcctagaga
    11281 gaaatctcta ggctttttat agtttttggg aataacgagt catatttgag catatctata
    11341 atcaaagaaa attttgaaag aaggggaaaa atttgattat atcagtaaaa gagattagga
    11401 attttttgat aaacatattt ataccactaa taataggtta cttaagtagc atcttaacaa
    11461 tgattatcag tggaacagat atttcaacct attatttaca actcacaaag ccaagctttg
    11521 caccaccagc ttttatcttt cctatagttt ggactatatt gtacatattg atgggtatat
    11581 cttcatacct aatattaaga aagggttaca acttacctaa agtaagagat gcaatttttt
    11641 attatggatt acaacttgta ttaaacttta tttggagtat actatttttt ggatttggac
    11701 ttagatttac agctttgata gatataataa taatgatttt ggtttctata ataatgattt
    11761 cgaagttttc taaaatagat aagagatctg gatacataaa tttgatttat ttaatgtggc
    11821 ttgtttatgc aggatttttg aactatttta tatggtttat taataaatag aatgtaaaga
    11881 ttttgtaaat taccctgtac ttttaataat atcataacaa acgaaaaata aatagactat
    11941 taaaaataat cattttcata tacaataata ttaatttaaa tacaataaat ttataaaaat
    12001 tgtatttaaa gattaacata ttaaagtatt agaattggaa aggcttgaag ggagaggttt
    12061 ttatgcaaat aatagtatct gggagacaaa tgaaattaac tgacggaata aaaggatacg
    12121 tagatggaaa attaagtagg ttagaaaaat accttgatcc tgaatcagaa gtgaaagtaa
    12181 cagtaagtgc gaaaaaggat agacaaaaag tggaggttac aataattcct attaacggtc
    12241 aaataataag agcagaagac gtagaagatg acttatatgc agcaattgat attgtttgtg
    12301 acaaattaag tagacaagtt gtcaaatata aaactaaagt taaagacaaa gttcaaaata
    12361 ataagagcat aagatttgaa aatcttgact ttatagataa tagcagtgaa tttgatgatt
    12421 atgattatga tgatgaagaa gatgagaata tagtaataga aagaagaaag aaatttaatg
    12481 taaaacctat gagttctgag gaggcgatac ttcaaatgga actagtagga cacaatttct
    12541 atatgtttag aaaccaagat aattttgaaa taaacatagt gtataagcgt aaagctggtg
    12601 gatatggttt aatagaacaa gattaataaa aattgtatac gtttagattt tatataagaa
    12661 aaaataaaaa ggtaatcatg ttgatttttt atcaatatga ttaccttttt cttgtttaaa
    12721 taatatatta gatattaggt atttattata taaaaatttt aaaactaatc tgttacaaca
    12781 tcaataaagt gcataaatac ctatttcaag cataaaatta attaaggggg taatgcttat
    12841 gttagaaata tccactgatt tacaagtaaa tcaaccagtt attactgtta caagtaatac
    12901 gtttattagt atagaaaatt atctatcaat attagaatat gaagttgatt taataagaat
    12961 aaagactaaa gttaaaacaa ttaaaatatc tggggataaa ctttcactta agtatattac
    13021 tgatagtgag ataggtataa aagggattat ctacaatgtt gagtatgttg attaggagga
    13081 gatacacttg ataagtttta taaggggata ctatgttata gttgtggaag gagtaggact
    13141 cgaacaattt ttaaatcatc ttataagaaa tgggataagt gtttacaatg taactagaat
    13201 aaaaaatact aaaatggagt ttcatataga tagacaagat ataaaagaat ttaagaatgt
    13261 ttatagagga agtaagtttg atataaaggt aaagcaaaaa actggggtcc cttttattat
    13321 caaaagagtt tataagcaca aaggtatgtg gatttgtgct cttgtttctt tatttttatt
    13381 gatgtctaca tcacagtttg tgacagatgt atatatacaa tctcctgaag gtataaaaaa
    13441 agaagctctt agaaatgagc tttataaggt tggagtaaga ccaggagtct ataagaagag
    13501 tattgataga aaagaagtta gagaccatat gatgtcaaaa tttaatgatg tggcatattt
    13561 atctatcaat gtaaaaggaa caaatatatt tgttactgtt acaaaaaagg cagaatccct
    13621 aaaatctaca gaccaatcaa attattgtaa tgtaattgct ttaaaaaatg gtataataga
    13681 aaaggtaatc cctagaagtg gtaaatcagt tattaaatct ggagacatag tccaaaaggg
    13741 agatgtctta ttaaatggag ctaataccaa gtcaatacca gaagtatggg caagtacttt
    13801 ttatgagtcc acaaaaaagg caagttatgt agatactgta aataaaaaaa ctggagaaaa
    13861 gaaaaatata tatacactaa gtttttatga taaagagttt acaatgagaa aaaatataaa
    13921 atataaagat tatgttgttg aaaataaaga aaaaaaacta tcaataggaa actatacttt
    13981 tccaataaag ataaaaacga gtacttttta tgaaacaaaa aaggttgaag taaaaaggaa
    14041 taaagaagag ttaaaaaaag aattatctga aaaagcttta aaagagttag aatacataat
    14101 cccagcttcg gctagaataa tagatgtaaa acataacttt aaggttaata aaaatatgtt
    14161 agaatattta ataacagttc aaactagtga aaatattgct aaaatttatc ctcttagtaa
    14221 gtcagaagca gaaaggttta taaaggaaga aagtaaacca gatgaaggtg aagaggaagt
    14281 tccttccaat ccagaaaaaa gaccattaaa tgatataaga aatgagtttg atgaggataa
    14341 taaggataat aaagatcaaa acaattctga tgaaaataat agtaatcaaa atagtaataa
    14401 tagtcaaaat aataatagca ataacaacta ataaaattaa acttaaaatc ttaggaggac
    14461 taattcaatt gataatacaa aagaaattta caatagcaga tggtaacttt gaaagagagt
    14521 tatttggcaa ctttgatgaa aatgtaaaat taatagagaa gactttaaat atagatgtaa
    14581 tacttagaga aggcaatata attttaattg gagaagaaaa aaatgtagat tctgcattaa
    14641 aacttatgaa tgaattacac caaactgtat caaatggtaa gcatttagat aagcagagta
    14701 tttcttattc attatctctt ttgcttgaag gaagtgaaca aaaaataaaa gaacttgagg
    14761 ggacaatcgt cataactcaa aggggaaaag ctgttcaacc taaaacttta ggtcaaaaag
    14821 aatatataaa acttatagaa aataatgata taacttttgg tgttggacct gctggaacag
    14881 gaaagactta tcttgcggtt gctatggcag taaaagcttt taaaagagat gaagttagta
    14941 gaattatact tacaagacca gctgtggaag caggagagag tctaggtttt ttaccaggag
    15001 atttaaagga caaagttgac ccatatttaa gaccactata tgatgcctta tttgaaatgt
    15061 tgggtgctga taaatttaat aagtatttag aaagaggtac tattgaggtt gcaccacttg
    15121 catttatgag aggtagaaca cttgataatt catttataat attggatgaa gctcaaaata
    15181 ctacttctga acaaatgaaa atgtttttaa ctagacttgg atttggctct aaagctgtag
    15241 ttacaggaga tgtaactcaa acagatttac cacaaaataa gaagagtggt ctaatacaag
    15301 ctacagagat tcttaaaggt gttccaggta ttgggtctat aatgctaaca gatagagatg
    15361 ttgtaagaca tgagttggta caaagaataa taagagctta tgagaagcac gacaaaagag
    15421 aagaatttaa aaaagaagaa cgtaaaaaag cgaaaatgca ggaaaagact ttcaaaagaa
    15481 agtaaaagtg tagtaatata gatatatagc attattaatg aataaaaagt aggtgtagtg
    15541 aatggatttg atactagatg atagacaaga taaattagaa gtaagtgagg agttaattga
    15601 aaagataaag gatataatca tagaatgttt agattatgaa ggatatgatg ataattatga
    15661 agtgagtctt tcttttgtag ataataaaga gattcatgaa ttaaatagag aatatagagg
    15721 agttgataga gtgactgatg ttttatcatt tcctctttta agtgatgatt ttgaggatgt
    15781 tgagttagaa gaagaatccc taggtgacat agtagtatct cttgaaagag ccttagaaca
    15841 gagtatagag tataaccata gctttgaaag agaagtatgt tttttaatat gtcatagtat
    15901 gtttcatctt ttaggttatg accacgatac agatgaaaat acaaaagaaa tgagagaaaa
    15961 agaagaacac atattaaata agttaaacat aacaagggag tagtttttat gaaaccagaa
    16021 aaaactcgtc aaggtataat taaagcattt aatgcagcta tagaagggat attgtataca
    16081 tttaaatttg aaagaaatat gaaaatacat tatttaggtt cagttgctgt tcttataata
    16141 agtttgtttt ttaacttttc aaaattagaa atgataatgt tacttatgtc catatgctta
    16201 gttgtagttg ctgaaatgtt taatacagct atagaaaaag ctgttgattt agtaactgat
    16261 gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag ctggaggcgt tctagtggca
    16321 gcactaaatt cagtagtagt tggatatatt ttgttttatg ataaattgac agatatatct
    16381 ggaatattaa tttataaaat aagagaatca gaattacata taaccttgat atgtatatta
    16441 ttggttctta tagctgttgt agttgtaaaa gcattgactt ctacaggtac tcctctaaaa
    16501 ggaggaatgc caagtggtca tgctgcctta gcatttgcta tagcaactgc aattacactt
    16561 atgactgaaa gagttgttgc ctctactctt gcatatataa tggctgtatt agttgcccaa
    16621 agtagaattg aaggaaaaat acataccttt tgggagacta ttgctggagc tttgttaggt
    16681 gtgttgatag ctatattagt atttcagctt ggaatgttct ataattaaaa taaatgtaca
    16741 taaaatattt aaaataaaac aaatctgtgc taattaaatt tttatgatat aatgtagagt
    16801 attaaataaa aataactttt aattattgta tacaggagga gttgttgtgg ataacataga
    16861 attgttaagg ttggcagaag atgccagaca acattcttat gctccttact caggttttag
    16921 agtaggtgca gctcttttaa ctaagagtgg taaagtttat actggatgta atatagaatg
    16981 tgcatcatta ggtggaacaa attgtgcaga aagaacagct atttttaaag caatatcaga
    17041 gggtgataag gatatctata aaatagctat agcaagtgat aactcagaaa ataatgagca
    17101 aacatatcca tgtggaatat gcaggcaggt aattatagag tttggttctg atataaaaat
    17161 aataacggga tatactaaag gagaggtgtt tgagcatagt ataaaggatt tgttgccaaa
    17221 ctatttctct ggaaatgatt taaaataaat ttaaaagtat aaataaattt aagtaattaa
    17281 aacaagaaga taaaaagtta ggagacagtt aatgttcaaa tcaggttttg tcagtatagt
    17341 gggtagacca aatgtaggaa aatctacttt aatgaataat gtagttggag aaaaaatagc
    17401 tataatgagt gataagcctc aaactacaag aaatacaata caagcagtat atacagatga
    17461 agaaatgcag atagtattct tagatacacc aggtattcac aaaccaaaaa ataaattagg
    17521 tgaatttatg gtaaaggcag caacagaagc ttttaaaaat gtagatttaa tattatttgt
    17581 agttgatgat tctaaaaaaa taggtcctgg agatagaaag ataatagagg atttaagaag
    17641 tgttaagaca ccaataattt tagttgtaaa caaaatagac caactagacc aaaaagatga
    17701 gctatttgat ataatcaaaa tgtatgatag agaaggcatt tttaaagaaa tagtaccaat
    17761 atcagcatta aaaggaaaga atacggatac cttaataaaa gttatacaaa attatttaga
    17821 agaagggcca aagtattttc cagattatat gattacagac cagccagaaa gagtattaat
    17881 agcagaactt ataagagaaa aagttcttca ctatcttaat gatgaaatac ctcatggtgt
    17941 agctgttgaa attgaaaaaa tgaaagcgag aaatgataag gaaatagtag atgtatctgc
    18001 tgtaatatac tgtgaaagag attcacataa gggaataata ataggaaaaa atggtaggaa
    18061 actaaaagga ataggtaagt cagcaagaca agatatagaa ttgcttttag gttcacaaat
    18121 aaatcttcaa ttatgggtta aagtcaaaga aaattggaga aatttacaaa attatataaa
    18181 taattttgga tataatgata aataaaggtg ggttatatgg ataggaaact ggatgcttcc
    18241 caagatttat tatatgaagt aaaatcatta attgataata ataaagtatt ggagttaaga
    18301 gaattaatag aggaatatca tataattgat atattcgata taatggaaaa tttagaggaa
    18361 gacatgaaaa ttcaattatt tgaagtactt cctttagata tggcatcttc tatattagaa
    18421 gaaggtagtg tagagttttt tattagtatt ttatctaaac tggatgtaga acactcgaaa
    18481 aatatacttg aacttatgtc tcttggagat atggcagaca agttgagtga acttgaagaa
    18541 gaagaaagag aacacataat aaatcttttg aatcaagaga atgccgatta tgtaaaagag
    18601 ttactattct atgatgaaga ttctgctggt ggaacaatga ccactggata tatttctata
    18661 aataaagata tgacagctct tgaggctata gaccatatga gagaagaagc tgaagaagct
    18721 gaaactattt attatatata tgtggttgat gatgaagaaa aactagttgg agtattgtca
    18781 ttaagagaat taataatagc tagagacgca aatatagtag aagatttgat gagtgaaaat
    18841 ataatctctg tttatgtaga tgaagataga gaagaagcag taagattagt atctaaatac
    18901 aatttaatag caattccagt agtagacaga caagaaaaat taaaaggaat aataacagta
    18961 gatgatataa ttgatgtaat ggaagaagaa gcaactgaag atatgtataa atttgcagga
    19021 agttcagaac atgaaagaga agttgctgaa aaagaaaacc ctacattgag agagcaaata
    19081 atatcagcac ttagaggacg attaccttgg ttaattatta ccttagttgg agggttgttg
    19141 gcttcgttaa tactttctaa cttagactat ataatgaatc ctgtatatgc tccattagta
    19201 ttttttatac ctgtagtaat tggtatgggt ggaaatattg gaactcaatc atcatctgtt
    19261 acagtaataa cattatctaa taaggattta aattttagca atgtggttag agaaggtatt
    19321 gtaggtatta ttacaggtct tttgtgtagt ataataacag gtattgtaat ttactttgtt
    19381 atgagaaagt tagacattgt attaattgtt tcaatatcat tatttataaa tatggtttta
    19441 ggtgcaacaa taggtgcgtt tatgcctgtt ttattaaaaa aaatggatgc agacccatct
    19501 acagtttcat caccaattat atctacagct cttgatataa ctggtatagc agtttacttt
    19561 ataataacaa cggcattatt gtcaaaaatt gtttaataac tttatgtatg atttatcctc
    19621 ttcttactaa aaatataagt ataaatgtaa gaagggggtt ttttaaaaat gaacaatatc
    19681 agttgggaag tttttaaaaa aacaggtagt atagatgcat atttatattt ctgtgattgc
    19741 aaaaacatac atgaagaaat tgaagaagta agggagaaaa aagaagacga tgataatcct
    19801 gaacacccag gggatagtac ttaaagctat aaggtataag gaaagtgata taatacttac
    19861 actatttaca agaaagcttg gaaaagtatc tgcaatagca aaaggagcta aaaaaaataa
    19921 gagctctttg ctttcatcat cacagttatt ttcttatagc aattttacct taaaaaaaca
    19981 aggtaatatg tacaaggtta ctcaaagtga aataataaaa agtttttata atatatccta
    20041 tgatatagag gcattttctt atgctacata tattacaaaa ttagttgaaa attctatact
    20101 tgaaaatcag actaataata ggctatttat attacttgcg cagactctgt atttgtatac
    20161 acaggataat acagacaata ggtttataac agcagcattc gagttaaaat ttttagacta
    20221 tataggattt aaacctatag taaataaatg cattaattgt gaatgtaaaa ttttacaaaa
    20281 ttctgtgttt aatatctacg aaggtggtat actatgtagt aagtgtagta aactttttga
    20341 taataatata aaattagact taacaactat aagtcttatg gaatatgtat taagaaatga
    20401 tattttaaca tgtagtaaag ctaaagtatc aaaatatata acacatgaac ttgagaatat
    20461 tctagacaag tatttgaaag tttatgttga taatattaat tttaaatcat tacacgtatt
    20521 gcaaagtgta aaaaataata agggagtgga taatgatgag tagtaatata actattgaaa
    20581 tggttgattc agtctttgaa agagttccag gagcaactta tgcagaagca aaagaagcat
    20641 tagtaatgtg caatggagat gtaattgagg cagtaatata tttagaaaat aaaaaaaata
    20701 cttgtaattg taaagcaaaa tctgctaaag aaactatgga agaagtattt ggcaaagatg
    20761 gagaaaatat aaagtttcaa ttaaaagagc ttcttagaaa aagtagtgta gtgagaataa
    20821 tagttgaaaa agatggaaaa actatgatga atattccttt aacagttgga gttgttggat
    20881 tagcttttgt accattagct actttagttg gattatcagc tgctgttata agtaaatatc
    20941 gtataaaagt tcaaaatgaa gaagatggag aaattgtaga tttaggtgaa ttgaatgaag
    21001 aaaaacttca cgttttgaag aatatgataa ctaatgcagc aaaagatgtg aaggatgtag
    21061 tagttgacaa aaaagaagat gacaaagatg taactgctga ccttatgaaa gaagaatctg
    21121 aaaagaataa agaagacgaa gacgaaaata aatagatata aagtgacttt taaaaaatga
    21181 agagaagata attataagaa ttatcactaa cattgacaaa ttttatagaa tttgatattc
    21241 taaaagttgt agaagaacta ttaaaatagc ctttcgtgta gacgagaggc tttattgtta
    21301 taggagggat ttttatgaat tttcaagaga tgatactggc gttgcaaaaa tactggtcaa
    21361 agcaaggttg tatcatgatg caaccttatg atattgaaaa aggtgcggga actatgaatc
    21421 ctaatacttt tttaagatca ttaggacctg agccttggca agtatgctat gtagagcctt
    21481 caagaagacc agcagatgga agatatggtg aaaacccaaa cagactatat caacatcatc
    21541 aattccaagt tatactaaaa ccttctccag ataatataca agaattatat ttggaaagtt
    21601 tgaaagagat tggaattgac ccaagtgagc atgacataag atttgttgaa gataactggg
    21661 aagctgctac agttggtgct tggggacttg gatgggaagt ttggctagat ggtatggaaa
    21721 taactcaatt tacgtatttt caacaagtag gtaatattga atgtgagctt gagacaggag
    21781 aaattacata tggattagaa agattagcta tgtacattca agaagttgat agtgtttatg
    21841 atttaaaatg gaatgataaa ataacttatg gtgaagtatt taataaagct gagtatgaaa
    21901 actcaatgta cgcatttgaa ttatgtgatg cagatatgtt gtttaatctg tttgatatat
    21961 atgaaaaaga agcattaaga cttatggaaa atggccttgt aataccgtca tatgactatg
    22021 tgttaaaatg ctctcatgca tttaatacat tggatgcaag aggtgcaata ggagttagtc
    22081 aaagagcttc atttataggt agagttagaa atatggcaaa gactgttgca gaaacttttg
    22141 taaaacaaag agaagaaatg ggattccctc ttttaaagga cggtgacaaa taatgaaaaa
    22201 ttatctttta tttgaaatcg gtgttgaaga aatgccatca agatttgtag gcagtacact
    22261 agaacaatta aaaaacaatc ttagtaaatt atttgatgaa aatagaatag cttttgataa
    22321 aataaatgct tatgggacac caagaagact tactttagta gtcgaaggat tgagtgatag
    22381 acaaagtgat ttagaagagg aaataaaggg tcctgctaaa aaaattgctg ttgatgcaga
    22441 aaataattta acaaaaccag cattaggatt tataaaaagc aaaggattaa gcgaatctga
    22501 catatatttt aaacaagttg gaaaagaaga atatatattt gcgactataa agcaagatgg
    22561 aaaagaaact tcagaagtac taaaagacat attaccagag actataaaat ctgtagtatt
    22621 cccaaaagct atgcgttggg gaggaaaaaa tcttaaattt gcaagaccta taagatggat
    22681 agttgcattg ttaaatgata aagtattaga gtttgattta gaaggaataa tatcttctaa
    22741 tgtaacaaaa ggtcatagat ttttaggtga aagtacaata gaagttgatt ctatagaaga
    22801 ttattttgaa aaattagaaa aaaattatat agttttagac caaaacaaga gaaaagaaat
    22861 gataagaaaa caatgtatag aagtagcaaa ttctttaggt ggagaagttg aatttgatga
    22921 agatttactt gaagaggtaa cttatttagt tgaatatcca actgcttttt atggagaatt
    22981 tgatgtggat tatataaaac taccaaaaga agttgttata actcctatga aacaacatca
    23041 aagatatttt ccagtactta aagaaggaaa actgttacct aactttatag ctgttagaaa
    23101 tggtgattct tacagaatag acaatgttaa agctggaaat gaaaaagttt tagaagcaag
    23161 acttgcagat gctttatttt tctatagaga agatactaag agaaacttag aaagctacat
    23221 agaaaaatta aaaactgttg tattccaagt aaaattagga actatttacg acaaaacttt
    23281 aagacttgaa aaattaagtg ctgatatact tgatagttta ggtttagtag atgaaaaggc
    23341 agatactata agggctgcta aattatctaa ggcagattta gttactggaa tggtaggaga
    23401 atttgatgaa ttacaaggtt ttatgggaaa agaatacgca aaagttggag gagaaaatga
    23461 tgttgtaagt gaagctattt ttgaacatta tcttccaaga tttgcaggag atatacttcc
    23521 taagactaat gcaggggttg ccttatcaat agctgataag ttggattcaa tagcaggttt
    23581 ctttgctata ggaatacaac caacaggttc tcaagaccca tatgctttaa gaagacaagc
    23641 tttaggtata cttagtatac ttatggacag aaaagttgct gtggatataa aaatacttat
    23701 tgaacatgct ttagacaatt attctgaatt agattttaat aaagaagaag ttgttgagca
    23761 aataatgaat ttcttcaatg agagagtaaa aaatctattt aaagatttag gaataagata
    23821 tgatgttgtt gatgctgttt taagttctga tataaatgat gtttctgata tgcatatgag
    23881 agctttagag cttaataatt ggctagaaaa agatgaatta gtggagatgc ttactgcatt
    23941 taatagagtt tctactctag ctcaaaaagc agaaagtgat aaaattgatg aatctttatt
    24001 aaaagaagat gctgaaatta aactttacaa tgaatttaaa caagtaaagg taaaagttaa
    24061 tgaattatta aaggataaga aatatggtat tgctttagat gaatttgcat ctttaagacc
    24121 agtagttgat aatttatttg atacagttat ggtcatggat aatgatgaag ctattaagaa
    24181 caatagactt gctttactta aacaagtata tgatactatg ctaggaattt gtgatttatc
    24241 taaaattgtt tataaataaa atttaaacta tttataagaa ataaggctgt ctcaaaatag
    24301 agatagtctt atttttcatt gttgagatga tgaaaatata tttgatttca tagaaaaaga
    24361 gtgtctcata aactaaaaag ttcattttga gacacccatg ttttttgtaa gtaaatataa
    24421 taaattatat tttatactta tcagttattt tgtttaaaat attatcattt gaatttaccc
    24481 attcataaaa attgcctgaa gatgcgtccg atattttata aagcatttca ggaatatcac
    24541 tagctttaaa atctccaagt gattttccca atctagtctt acctatttca aaactgcctc
    24601 caacaaataa ttcaaatgca tctgtagata taccatcaac attcttcttt ttaccacata
    24661 aacctatgct accaacttga tgaacaccac atgaatttaa acatcctgat atatataact
    24721 taggtgcttt atttaaaatt tcttcattat tttgtaatct aaaataatct atgatttcat
    24781 gtaacatttt ctgactattt tgtatgccca tttgacaaat tggaacacct atgcaagata
    24841 tactttgttc taattgagaa ttacaactaa tagttttact tatttctaat attctttttg
    24901 cttcatttcc atttaaattt aaaatatata atccctctgt cattcctagt ctaatcatag
    24961 gattttcaac attatctaat tctttaagta aagtacttaa atcctttgtt aacaaaatac
    25021 ctccaactgg atgtatatat acagtgtata aaccatcctg tttttgaggt attagtctag
    25081 aatcttgtat atctatttta actccaggtt tagagtaatc tatttcttca atattgagtt
    25141 caagactacc attttctttt tctaatttat agtattcttt aaatttttct aagaatagtt
    25201 cttcaccaag tgattcgacc atatatctaa ccctagctct gtttttattt tcataatttc
    25261 cataatccat aaagaattta atcatacctt caacacaata taaggcgtct ttcggcttta
    25321 taagttcatc aagttctaaa gcaactttgg gttgtttacc tagacctcca cctaagaaaa
    25381 ctctgaaata tggttcatca tttttcaaag tagcaacaaa tcctaagtct tgtatactac
    25441 aatgagcagt atctgtgtaa caattagaat aagaaacttt taattttcta ggtaaatggt
    25501 atgtagtagc atttttgata aaatatttat cagtagcaac tgcatatgga gtaacatcaa
    25561 agacttcact agggtcaact ccagataatg gagataatcc gacatttcta ggataattac
    25621 caccaccacc tctagtgaat atatcatttt ttattccttc ttccatgata tcaacaattg
    25681 aatttataga taaatcgtgt aattgtatag cttgtctagt tgttaagtgt atcttatcta
    25741 agttgtgttt aatagctatt tcatagattt tattaagttg atactgagat aaaactccag
    25801 cagatactct aagtcttatc ataaaagatt ttttgtctct ttgagcataa acaccatatc
    25861 caccagaaaa tcccttatat tgcattttat ttatttctcc atttaaaaat tttgaagaat
    25921 attctttaaa attaggtagt tcatttaaca atatcttttt aaaattatcc attatttcac
    25981 ctcattgtta gtcttgtata aaaatatatc tattgtataa tagatatata tattattatc
    26041 tagtcaggat aaaaatataa cagaataatt ctattaaaag taagaataat aaggacatag
    26101 tgagtatttt gtttttattt tacaaattaa gtataatatt tttgttttat aaaatataat
    26161 taagataaat agataatttt gtaattttaa tatataatat ttagtagagt gtgggagtag
    26221 cacttaatat ttgcaggagg agtttttgta tatgggagaa ataataaatg cattagatag
    26281 agccctagac ataattttgc tgttatatca tgaaaagaga gaaatgggaa taactgaaat
    26341 aagtaaggct atgggagtat ataaaagtac tgtacataga actttggtta ctcttgaaaa
    26401 caaaggtttt gttatacaaa atgcagagaa tggtaagtat tggctaggaa taaatttata
    26461 tgcaataggt atggttgtgg gagaaaaaat gtcattgact gaaatagtaa aaccatatac
    26521 gaaaaaatta aatcaagaat ttaacgaagt agttaatgta tctatattag aagaaagagc
    26581 tcaagattct ccaagaagta tcataataca taaagagtat ggttcaaatc aattactttc
    26641 agtaaatcca tccgttggtt caagtagtga atgttattgt tcagcagttg gtaaatgtct
    26701 tatggcattt aatgattcta tagattttga aaaatataga aaaacaccaa tacacaagta
    26761 tacagaacat acaattgata attgggatga tatgatgctt tttttagcaa aaataaaaga
    26821 acaaggatat gctatagatg atgaagaact agaacatgga ctaacttgta ttggagcacc
    26881 tatcttagat aaaaataata aagctattgc agccataagt ttgtcaggtc caactataag
    26941 aatgagagaa ggggactttg agtataagat taaaagagtt atagaaactg caaaaagtat
    27001 atctgaactt tttagatgat ttttaatctc gataataaaa ataggctata tttaaattgt
    27061 tatttgtaaa tatcagttta aatgtagcct atttttcttt aatctaaaat taaatattct
    27121 taaaatttaa aatgttattg aaatatataa gcgaatgtgt tatcttttaa ttaagcaata
    27181 aaaatagaac aaggttctat actatagaac aaaattgatg catatatagt atttatacaa
    27241 aatataaata ttatatattt aaaattaagt aaaaatatta cataaagggg agatttgaga
    27301 aatggaaaaa gtcaagttga gtgttgaaag atgtaaggga tgctatcttt gtattgaagc
    27361 ttgcaaaaaa gatgcaataa gtctttctga ctacacaaat aaaaaaggct ttgtaactat
    27421 taaagtcgat gaagaaaaat gtattcaatg tggcgcttgt tatacaatgt gccctgattt
    27481 agtatttgag atattataaa ttatttttat tagaggggag aaaaggtatg caaagtttat
    27541 taattaaagg aaatgaagct ttagcagaag gtgctatacg tggtggttgc agattttatg
    27601 caggttatcc aataacgcca caatcagaaa tattagaaat gatggcatgg agacaaaaag
    27661 aagttggagg agtatttatc caaggtgaat cagaaatagc cagtgtaaat atggttatgg
    27721 gagcaagtgc acttggagca agagctatga cttcttcatc tggtccagga ttttcattaa
    27781 aacaagaagg tattgcatat tgggtatcag ctggtttgcc agcagttgta gcatgtgtgc
    27841 aaagatatgg tataggtgat ggatttatag gagctggtca agataattat tggcaagcag
    27901 taaaaggcgg tggaaatgga gattatcatt taatcgtgtt agcacctaat agtgttcaag
    27961 aaaatatgga tatggcatat gaatcttttg aattggctga aaaatatatg catccagtac
    28021 ttatactttc tgatgctaca ataggtcaaa tggtagaacc ttgtatagca ccaccaatga
    28081 aagaatacga tatggataaa gctccatggg ctgcaaaagg aacacctgta ggagaaaaaa
    28141 ataaagtaat aactgatata acatattttg aagaccaaga tacatggcaa aaagggtata
    28201 tagaaaaaat gagaaaagtt caagaagatg aacaaagatg ggaagagata gaaatagaag
    28261 atgctgaagt ggtatttgtg gcatacggta tttcatcaag aatagcagaa ggtgcagtta
    28321 gagatgctag agcaaaagga atgaaattag gtctaataag accaaaaaca ttgtggcctt
    28381 acccaagaaa agcatttaag aaattaagtg attcattgaa gggacttgta acagttgaaa
    28441 tgaacatgat ggcacagatg aaggaagatg taattatagc atgtgataat agatttccag
    28501 tatattcagt agcaacagct caatatatgg cagaagtaaa tcaattaata gattttgctc
    28561 aacagattat agatggaaaa gctaagcaag aggaggtatt ttaattatgg ctaaaaaagc
    28621 accagcaata atgatgtgtg aaaatatgtt ctgtggaggc tgtggacact ctatagtcga
    28681 tagaattatt ggagaagtat taacagaaat ggatatatgt caagatacaa ttatatgttc
    28741 agatataggt tgtaatcata tgtttcaatt tagtatgaat gtagatgttg tcgttccacc
    28801 tcatggtaaa atgggagcag caatggcagc tatgaaaaga gttagaccag ataaatatat
    28861 atttacaata gctggagatg gtggagctta tgcaataggg cttggagaaa ctatgtcagc
    28921 tgctactaga aatgataatg taatatttct tgttatgaac aataatatgt ttgcaatgac
    28981 aggagggcaa atggctccaa ctactataga aggtcaatat acagcttcta ctccagcggg
    29041 tagaaattac gcagatcatg gttcaacatt tgatgttgta aaagttatgg gaaatttaga
    29101 tatagcatat ctagcaagag gaacaataac taaccctaaa gaaataaata caacaaaaag
    29161 atacatcaaa aaagctctag aaaaacaaaa agaaaagaaa ggtttttgct taatagaagt
    29221 gcttgtacca tgtccaacaa actggggatt aaagccagta gatgctatga agagaattga
    29281 aaatgaactt gttccaaagt atccacttgg agaatttatt gattagggag gaaataatat
    29341 gttgaatgaa attatatgtg ctggttttgg aggacaggga gttcttacag caggaaaaat
    29401 tataatgtat gttgcatata aggcagaaaa gaaaattaca tggttcccat cttatggaaa
    29461 tgagatgagg ggaggaagtg caaattgcaa tgttgttgta agtgatgaaa aaattgcaaa
    29521 tccttgtgta aaaaatccag atatactatt gggaatgaac agcgtatcag tagataagta
    29581 tgaaagttta atgaaaaaaa atggttatat gtttgtcaat gaatcactag taagtaagga
    29641 tagaaagtac agagaagaca ttaatgttat aaaagtacct gttactgaaa tagcacaaaa
    29701 tttaggcaat gaaaaagctg ctaacatttg catgttaggt gctatggtaa agagtttgga
    29761 tttatttaca aaagaagaat ttgaaagtgg tatgtgtgaa tactttgaaa atcaaggcaa
    29821 aggtaaattc aattctaaaa atgtagaagc atttgaagct ggatacgact acgaatgaat
    29881 attttaaata tttaaaataa ttattgaaat acttattttt atatgttagt atttaattaa
    29941 taaaatatag aacgtcgttc tgaacaatag gacgaagtct ttaggtcgga atacaagaac
    30001 gacgttctgt atagtagaac tagggggtgt taaattgaaa gttttagtaa taaatcctgg
    30061 tggtacatcc acaaaaattt ctgttttcca agatgaaaat gaaattttga aaaaaaatat
    30121 aacccataca agagaagatt taaaaaattt ttctaaagta tttgatgaat atgattatag
    30181 aaaacaactg atagtagata ttttaagtag tgagaatcat agtataaatt cttttgacgc
    30241 agtagttggt cgtggtggac ttatgaaggc tataaaaggt ggtacctata ctgtgagtga
    30301 agaaatgatt gaggatatga gaaatgaaat aaatggggaa catgcatcaa atttaggtgc
    30361 attattagct aaaactatag ctgatgaaat aggagtacaa tcatttgtag tagacccagt
    30421 ttctgtagat gaatttgatg atgtatcgag gattacagga atatcagaca tagaaaaagc
    30481 aagttggtta catgcattaa accataaagc tgtttcaaga aaaattgcac aaaaactagg
    30541 gggaaaatac gaggattata actttattgt agctcattta ggttcaggaa tatcgattgt
    30601 agcacataaa caaggcaaaa tgattgatgg aagtggtggt aggtctgatg gtccattttc
    30661 tccagaaagg agtggtggat tacttgcata tcctcttata gaattgtgtt actccggaaa
    30721 atatactcat gatgaaatgg tcgcaaaagt aagttcgatt gggggaatgt acgattattt
    30781 aggaacaaag gatatgatag aaattgaaaa cagaatcaag gagggtgatg aaaaggcaaa
    30841 acttataatg gatgcattta tttatcaagt agcaaaagaa atttctatgt atggagcttc
    30901 actttcaggg aatgtagata gagttatctt gacaggaggg atatctcact cagaaatggt
    30961 agtcaaagga gtagcagaaa aagtttcata tctagctcca atagagatag taccaggaga
    31021 gatggaaatg gaggcacttg ctctaggggc acttagagta cttaatggag aagaaatagc
    31081 aaaaacttat taatatttag gagaaagagt atgttaaaag attttaataa tttattagaa
    31141 gaggctaaaa agcttgaacc tgttattgta agtgttgctg ttgcagaaga caaagaagta
    31201 ttaaaggctg tgaaatcagc aacagattta aacattataa agccaattct tgtaggagac
    31261 aaggataaaa tagaaagtat atcaagagaa attggattaa aaggtcatga aataataggt
    31321 tgtcaaaatc ccgatgaatc agttaaaaaa gctgtagaga tagttaaaaa taaggaagct
    31381 aaagttctta tgaagggatt agtaaataca agtgtctata tgagagctat attaaataaa
    31441 gaaacgggtc ttagaacagg aagattatta agtttgcttg cagtatatga attacctcaa
    31501 tatcataaac ttctttattg tactgatagt ggaataaatg tatcgccaaa cttaaatcaa
    31561 aaaaaagata taatgacaaa tgcacttctt actatgaaaa gtataggcat ggaaaatcct
    31621 aaaactgcga tacttactgc aaatgaaatg gtagacccta agatacaatc tacagtggat
    31681 gccaacctac ttgtagagat ggaaaaatcg ggagaaatac caaagtgtat agtggaaggt
    31741 ccaatagcat ttgatgtagc atttgatgca catgcagcag aacataaagg tattaaaagt
    31801 aatattgctg gtgatgttga tttattagta tttccaaata tagaagcagg gaatatatta
    31861 ggaaagtcat ggcttagatt taataaagct aagtgggcag gtatagtttt aggagcaaca
    31921 aatccgataa ttttaggttc acgttctgac acagctgaaa taaaaataaa ttcaatagca
    31981 ttagcgtgcc tagcatcaca ataagaaatt attattaagg aggaaaaagt attatgaaat
    32041 atgcaaaaag aatggatatg gtaaaagctt cagctataag agattctcaa aagaaaatag
    32101 ctcagaaggt agcaagtggt ggaactgtta tatcttttgc tgcagggctt cctgacccaa
    32161 atttatttcc aatagaagag ataagtgaaa taactcaaaa agtacttaat gaaaggggaa
    32221 aatttgccct agcatatgga cctactaaag gtgaagatga gttgttagat ctattagtaa
    32281 aaagaatgaa agaagaagaa aatattgata caaaagcaga aaatattata ataactactg
    32341 ggtcacaaca agggatagca ttaagtgcta tgattttatt agaaaaggga gatgtagtag
    32401 ttacagaaaa tccaagttac ttaggtgcaa taaatgcatt tagaccttat gaatgtgatt
    32461 ttttaggagt agatacagat gaagaaggaa tggtaacaga tgatttagac aaattattat
    32521 ctgaaaattc taacataaag gtgatatatg taataccaac atttcaaaac cctacaggaa
    32581 aaacatggtc tttacaaaga agaaaagatt ttatggaagt cgtgaataaa tatgatgtca
    32641 tagtaataga ggataatcct tatggagaaa taagatttac accagaacct ctacctacac
    32701 ttaaatctct tgatactaaa gataaagtat tgtaccttgg gtcattttca aaggtattat
    32761 gtccaggatt tagagttgct tggatgtgtg gtaatgtaga catcatagat aaggcagaac
    32821 ttttaaaaca aggtatagat ttacagtcaa atcaattttc gcaagtacaa gtaatagaat
    32881 ttttaaagaa atacaactta aatgaacata tagaaaaaat aagagcagag tataagaaaa
    32941 gatgtctgct aatgttagat gctatgaaga aacatttccc agaggaagct aaatatacag
    33001 aaccagatgg aggaatgttt atatgggttg aacttcctga aagtataaat gtagatgatt
    33061 tattagataa agccattgat gctggagttg cttatgtttc tggagagtcc ttctttgcaa
    33121 ataatggtcc aaaaaatact atgagactta actatacaac aatgtcagaa gaacaaatcg
    33181 ttaaaggtgt tgagatactt gcagaagtta taaaaaaaga actcaattat gcttgtgaat
    33241 aaatataaaa agtgagggtt taaatatggg agaaaaagaa aacggtataa agtttggaaa
    33301 gttaatgttt caacttgtta ttatagcaat atcttgggaa ttagtttata ttataccatt
    33361 tatccaatat acattatatg atccaattct aaaagcatta gaatgtacaa acactcaact
    33421 aggttttcta cttacaattt atgggttagg taatatattt ggagctccta taggaggctg
    33481 gttagcagat agatttgatt atagaaagat aattttaggt tctgtatttc ttaatggagt
    33541 agtatcattt ttatttgcat ttaatatgaa ctatccattt gcagttgcta cttgggtagg
    33601 ttgtgcaatc acatcattag ttatgaacta tccttctatg gtaaaaatac ttagagttat
    33661 aggaaaagat aatcaaggta aggtttatgg atttaatgaa gctatggttg gagtttctgg
    33721 agtagtaatg ggagcaatat tcttatatat ttatacatgc tttgcaactc ctacattggg
    33781 tatgagatgg gtaatgatat ctttaggaat attatcaata gcaatgtgtc cagtattatg
    33841 gtttgtaatt aaagatgtag atattaaaga agaaagagaa gaagcaggtg aaaaaatgtc
    33901 tgctggtgat tttataacag tactaaaatc accaaatact tggctagtag gaataagtat
    33961 attctgtgta tattcattca cagttacaat gtcttatttt acaccatata taacatctgt
    34021 acttggaggt tctgtagcat tatctggtgc attagcaata ataagacagc atggtctaaa
    34081 attatttgga gcaccatttg gaggatattg tgcagacaaa ttaaaatctc ctacaaaagt
    34141 acttttacca atatatgtat ttggtatagc agtaatagtt ttattcctag tattacctgc
    34201 atcaactcca atgactatat tcatagcttt aacatttgta gtaggtatat taggttacat
    34261 gggaaaaggt atatattatg cagtacaaga tgaagtaaaa gttccagtta aatattcagc
    34321 aactactata ggtatagctg cagctttagg attttcacca gatgtattcc aatttgcatt
    34381 aataggacac tggatagata cttatggcaa taaaggttac acatatacat ttatattcca
    34441 aatagcaatt ctagttattg gaatactatc ttgtttatat atattaaagg ttaaaaagag
    34501 aagagcaagt aaattagaag cttaaatatt caatttggta attaagtata tataaataca
    34561 atacttattt tgatgaatgt gattgtataa ataacataaa aaatatcaat agattaagtt
    34621 ttataataat gtttagagaa taaagtataa aaatcaaaat gttatcaagt tttctaggtt
    34681 ttaacataaa gttacccctg aaggtgtaaa attgagtggc aattattatc tattttctta
    34741 cctacataaa cctatttaat ttaaattcta agagatttta aaaatagatg gtagttgtac
    34801 tcaattttaa acctcataat aaatacgaaa atttaaaatc taactagata ctatttaatt
    34861 atatattaat aaatatatat tgtactattt atataataat aatttcaggc ttttcattat
    34921 atgtattcca atttatattg tttgattatt attcaaacac acgtcgttaa aaattttata
    34981 aatttatgtt tagatatgat taatactctt gattttagaa ataaatattt gtagattgta
    35041 gattaaagat aatataaata aatagaaggt agatttttta gatatattaa tataaagatt
    35101 atataaaata ttgaaatata gctgtttaaa ttattgtcta taaatataaa atgaatggtg
    35161 gtgagttaag tgaccgattt agttaacacc attaatggaa ttgtttggtc acccgtatta
    35221 gttgcaatgt gtttgtgtat aggattatat ttttcattga aattaaaatt ttttcaaata
    35281 agagatgtaa aagaaatgtt tactctcttg cttgaaggag atagttctca tgttggtata
    35341 tcatcatttc aaggttttgc tacatctatg gctggaagaa tagggactgg aaatatagct
    35401 ggtgtagctg tagcaatagc aatgggaggt ccaggagcat tagtatggat gtgtataatg
    35461 gctacattag gttcagcaac tgcatttgta gaatctactc tagctcaaat atataaagat
    35521 gagcatgatg ggcagtatag aggaggacct ccatattatt ttgaaaaagg tcttgggtgg
    35581 aaaaagtgtt cagttttatt ttcaatagtt gcaataataa gttatacatt ttttatgcct
    35641 ggtacacaat caaacacttt atatctagca atgaatcaag catttggatt ttcaaaaatg
    35701 acaatagcag tagcatcttc tattgcatta gcgataataa tttttggagg aattaaaagg
    35761 attggtttat ttgctgaaaa agtagtacct ataatgggtg gagcatacct tattatgaca
    35821 ttagtaataa ttataacaaa tattagttta gttccagagg ctattgtaac aatgtttaaa
    35881 tctgcatttg gatttggttc agtttttgga ggaatgctag gttcagcaat atcttgggga
    35941 gtaaaaagag gtatatactc aaatgaagca ggagaaggtt ctgggactta tggtgcggca
    36001 gcagcagaag tgagtcatcc agccaaacaa ggattagttc aagcattttc tgtttatgta
    36061 gacacattat taatatgtat ggctacaggt attatgattg taataacagg aatgtataat
    36121 gttacaggtg tttgcacagc actgccaggt gtagaaccgg gaccacaata tgtacagagt
    36181 gcaataaata cattacatcc acaattaggt gatattttta tagcaatagt tttattatta
    36241 tttacattta caactttatt agccttctca tatatgatgg agactaatgt atcttactta
    36301 gtaactagaa ttaaagcaga aaaacatgaa aagtacataa ttaatgtctg tagatttatg
    36361 cttatagcat gtgcagcaat gtgttgtttt atagaaccac ttacaagctg ggcattaggt
    36421 gatatatgta taggtgtatt aacatatata aatttaattg ccatctttac actaaaaaaa
    36481 ccagctataa aggcatatta tgattatata agacagaaga agatgggaat aaaaaggagt
    36541 gatagaactt ttaaccctat agagttagat ataagaaatg ctgagttttg ggaaaataga
    36601 tacttaaaag aggataagca tgagttaaat taaagttata ataaaatata aaaattacta
    36661 aataaattta gtgggaggta agattggtat gaaagaaaga ataatacaag tggctgattc
    36721 aaagaaagaa aaaattctag ggctatgtca atctttatat gatgaaccag aaattgcttt
    36781 gcaagaatat aaatctgcta aaaaaatatc agaattttta agagaagagg gttttgatgt
    36841 tgaggaaaac ttggcaggta tggctacagc ttttaaagca accaagaaaa atggagatgg
    36901 tcctaaaata gcatttatag cagaatatga tgctcttcca ggcaatggac atgcatgtgg
    36961 acatcattta attgcatcta tgggagttgg agctggaata gctctttcaa gtatactgga
    37021 tacttacaag ggagaagtaa gtataattgg aacacctgct gaagaaactg gagatggaaa
    37081 gccttacttg atagaacatg gtgtatttga tggctatgat gcagcaatga tgatacatcc
    37141 aaattcaaaa acttgtgtaa cacctgaaat tatagcaata ggaggtctag atttcatatt
    37201 tactggaaaa gcatctcatg caggagctaa gccatataac ggaataaatg ctttagatgc
    37261 agtagtatta ctatataata atataaatgc actaagacaa caattagtag atggaacgag
    37321 aatacatggt attatactag aagcaggaac tgctgcaaat gtaatacctg atatgggaaa
    37381 agtaagatta gagataaggg caaaagaaca aaattacttt gatgaagtag tagaaaaagt
    37441 gaaaaattgt gctagaggtg ctgcaatagc cactggatgt gaacttgaat tttatcactt
    37501 tgaaccaact tgccaaggat taaatgaaaa taaagttttg gtagacatat ttacaaagat
    37561 tatggaggaa tttggaatat atgaagatga acaagtaatg ttaggttcaa cagatatggg
    37621 aaatttaagt caaataatgc catgtataca tcctttgatg aagttttctg aaaatggaga
    37681 agaactacat acaaaagaat ttttggaggc ttctataaat tcatatgcaa aagatagagt
    37741 aattgatggt ataaaaatat tagctttaac aggatttaat ttatttgaaa atccagagtt
    37801 attgaaaaaa atgaaggaag agtagaactt agactatttt taattatact ctttaaaata
    37861 agaagagtgt ctcaaaatga actttttagt ttatgagaca ctcttttttg tatgaaatta
    37921 aatatatttt tattatttta acaatgaaaa agaggcttat ctgtttttat atgacctttt
    37981 ttaacttgta gcacaaaata tcatgaatga ttacaattcg aatgataaaa tcgattataa
    38041 gatatatagg aggggtacat atggaaactt tagaagtaag aaatattgaa ttgagtggta
    38101 caaattatga gataggatac agactaggag aattggttgc taatatgcca gagataatag
    38161 aaggtcagat taataaatct aatctggtaa gcaaaaaaga agaaaaagag atgatagagt
    38221 tgtttgataa atattgtcca ggtcttaatg aagaattgca aggttttgct gatgcaattc
    38281 aagtgaattg taatcaaatt ttatattata ctatgactta tcttaaacca ggttgtagtc
    38341 aagttgcatt agcaccagaa ttaactgaaa atgggcatgt actatttgca cgtaatttcg
    38401 atttctcaca caatatggag gattttgttt tatgtaaaac taaggtaaat ggaaaatatg
    38461 cacatatagg tactaccgtt atgcaatttg gaagaggtga aggtatgaat gaatgtggct
    38521 taggtgtaag tcagtcatca tgtggtattc ctgtaggcaa ttcagatggt ttaagaaaac
    38581 cagcaattgt aggtttacag ttttgggctg ttattagata tttgttagaa aattgcaaag
    38641 atgtagatga agcattagaa tatttaaaag atatgccaat tgcatacaat attaatttat
    38701 tgcttgcaga taagtctgga aacattgcac ttgtggagac tttagatggg aaaaaagaag
    38761 taaatataat caatagttta gaaagtaaaa gagaatattt tttacactct actaatcata
    38821 cacatattga taaacttcat aaattagagc cacaatcaat gaaaaattct attcatagat
    38881 ataaattaat taaagaatat ataaataagt ctaaaaaaat tggagagaaa gagttaatga
    38941 atttattgtc ttcaaaatat ccaaatggct tatcttgtaa ttactataat gatttttttg
    39001 gaactttaaa aagtatagtt atggatttaa atataggaaa gtttaatatt ttatggggtg
    39061 gactagagaa taaatgggag agctactatt taaaaaatga cattaaatct ataacacaga
    39121 ggataaacat aaatattgaa aaagcaccat ctgacttttt tgatttcatt gaataaaaaa
    39181 tgtatttaat tattctaaat aattaggata tataaatcaa ctaaaagttt ttatttatcg
    39241 tattgaatta gttataatat tttagtatag taaaaaatag tgctatttta catatttaaa
    39301 gagtttaagt agaagagagg tgccatattg gattacttaa agtattttaa aaaagctgta
    39361 atgtatattg agaataattt acatgaaaat ataactgtaa atgatgtggc aaaagaaact
    39421 ggatattctt attatcattt aacacgctta tttaagtcta tgtttggtga gagtgtagga
    39481 agttatataa aaaaaagacg attagtaagt agtacaaaag aacttttata tagtgataaa
    39541 aaagtgatag atatagctat tggtagtgga tttgaatctt cggaagcatt tagcagagca
    39601 tttaaatcta tatacaaggt tagccctata gaatatagaa aaaatagaat tgatgtattt
    39661 gttggtaaaa agaaaaaatt agagttagat tttatgagac atcttgttgg aaatataacg
    39721 attaaaccag ttataaaaga aatagaaaaa ataaaggtaa taggtataaa ggataaagta
    39781 attttagaaa ataattcatt acctgacttg tgggagaaat tcagaaaagt acatcatatt
    39841 gtcccaaata ctttgccatc aaagagagtt tttggaatct gtgaagcaat ctctgaaata
    39901 cacctagtta gtgaaagtat ggaatttaat gagataatag gacttgaagt taatagttat
    39961 gatgtgattc caaattcatt tgtttcaaaa actattaagg aaggaaagta tgctatattt
    40021 actcatactg gtagcttaga taatttagac aaaacttatg aatatatatg gggtacttgg
    40081 tttttaagtt caaaagaaga acttgatata agagatgatt ttgaagtata tgatgaacga
    40141 tttttaggtc ctagtaatgt gaactcacaa attgatattt atatacctat aaaataatct
    40201 ataagaaaac taaaaaataa tttgattaag aaatagatgt tcgtaaaaga gcatctattt
    40261 tttattttta tcagatttag ataattttaa taataaaaga aata
//
LOCUS       DAMJVI010000025        40080 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.025, whole genome shotgun sequence.
ACCESSION   DAMJVI010000025 DAMJVI010000000
VERSION     DAMJVI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40080
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.025"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            280..735
                     /gene="smpB"
                     /locus_tag="PC545_001416"
     CDS             280..735
                     /gene="smpB"
                     /locus_tag="PC545_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDF2456191.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
     gene            893..1242
                     /gene="ssrA"
                     /locus_tag="PC545_001417"
     tmRNA           893..1242
                     /gene="ssrA"
                     /locus_tag="PC545_001417"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1357..3384
                     /locus_tag="PC545_001418"
     CDS             1357..3384
                     /locus_tag="PC545_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDF2456192.1"
                     /translation="MSIDNNFNHVAFLWNIAESLRGTYKEEDYRKVMLPLIVIRRFDC
                     LLDDYDREIIKSVYEEYDFLPEEERDELVIVDLKENHDINLQFYNVSDFTWKKLLDDS
                     ENIKSNFEEYLNGFSNNVKEIIGKFKFKDEINQLDKKDKLYAVLSKMYEVDLHINSVS
                     NNKMGYIYEEMLRRFTENSAAGEQYTPREVIRLCMEILFMGKENFLTEEGKVISIADF
                     CCGTGGMLSIAEDYVEKINPSAIVNVYGQELLDESFAICQADMLMKGQNPDNIRLGNT
                     LTQDRFNGEKIRFLISNPPFGVTWKDEEKKVKEEADLGFDGRFGAGTPRVSDGSLLFL
                     QNMISKMYDDEEGSRIAIIFNGSPLFTGDAGSGESNIRKWIIENDLLEGIIALPTDMF
                     YNTGIATYIWVLTNKKEAKRKGKIQLVNASEYYQLMRKSLGNKRKEISLEQIEQIKEI
                     YEKFEESENSKIFDNEDFGYRKVTIERPLKLSFKVNEEAIENVKNTTQFINLAVSKKK
                     DIEVKEKEEAEGKIKQDKLIKLLESFDNTLEYMDRDKFIKDLKAKSKEFDIQLSAGLI
                     KAIVNTIGVRNEDAEVCKDSKGNIESDSSLKDTESIGLKDDVYEYFEKEVKPHVNDAY
                     IDESSINNIGYEIPFTRHFYKYEELRSFAEIMKEVESLESEIALKIRKVLG"
     gene            3384..4775
                     /locus_tag="PC545_001419"
     CDS             3384..4775
                     /locus_tag="PC545_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDF2456193.1"
                     /translation="MGYRYRSDDELKDSGVEWLGKIPKDWEVRQLKRVLSDRKENNEP
                     IKTSNILSLTIEKGVIPYSEKGTGGNKAKEDLTQYKLVYPNDIVLNSMNVIVGAVGLS
                     KYFGCASPAYYMLYKINANDDIRFYNYIFKSKLFQDSLKGLGNGIMMKQSETSGKLNT
                     IRMRIPMEKLSSVKIPIAFNLDQEKIANFLDEKTSQFDSIISKKEKLIERLEEAKKSL
                     ISEVVTGKVKVVKTDDGYELIKRSSEEMKDSGVEWLGEIPKEWDIKRLRFLGKLQNGI
                     SKSSDSFGYGYPFVSYSNVYRNIELPHIVEGLVNSTEEERKIYSVEEGDVFFTRTSET
                     VEEIGFASTCMKTIDNSTFAGFLIRFRPNDKRLYKGFSKYYFRCDLNRKYFVKEMNLV
                     TRASLSQNLLNNLSVVLPKYEEQVEIYNALELKVSYINLSINKLKYQIEKLKEAKQSL
                     ISEAVTGKIEILD"
     gene            4837..6108
                     /locus_tag="PC545_001420"
     CDS             4837..6108
                     /locus_tag="PC545_001420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456194.1"
                     /translation="MDKNIFQININTDVLIRLRNQINEEYYISYRKEFYDKNNKKHRA
                     WDKICAIMDRLDDTVDYLNKLKLNTGKYRRSAFDFFEFMNQASVVMDCITHLAKIFEV
                     PYEEVETSTEIFNELGKEGKGTDKKYFEYLRSLCSVHPMETSRHKIYQENSFECSPYV
                     VWNDKSIWTNDASDLYAVVYTSKDNDSFKRIHIYMNQIFEYVKRRVNFIENIIEHIEK
                     YQQEIIEIFRNTPIKKEEDFESYIDYLRNLKKEIEIRYGNDSFHELNYLIELFDLPLS
                     NPDNEEKMNLYKNALKYAIKFEHNSIQNMTNEGYKNNGILYPQVNITTTLLEELISLN
                     SGSEERRKYRYAIEKTSYLSYDSCWSDREWAYDQIDDMMPFLERYVTFDKAQSDFEHY
                     VLVKLALYLECLENECLINKNVPNDKKFRIK"
     gene            6143..9124
                     /locus_tag="PC545_001421"
     CDS             6143..9124
                     /locus_tag="PC545_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDF2456195.1"
                     /translation="MLPKEKHLEESIENHLVSIGYEQGHSQEFDLENCLFVDDLFRFL
                     ENTQKELLDEFKYNRGSNYKKAFIDLLNSNIKRRGLISVLRNGVEDIMMNGKFQLFYN
                     KPNLTTNKTAYENYKQNIFKITRQLKYSSKHNNTLDIVLSLNGFPVIVMELKNQFTNQ
                     NVYNAIKQFKKDRNPKETLFGFNQRVLVYFAVDTDEVYMTTELKGDKTFFLPFNKGNN
                     RGKGNPVVEGKEKTSYLWEEILTKDSLLDIIKRFYFIQEDDKGNQRAIFPRFHQLDAV
                     RKIVEDLKINKVGKNYLIQHSAGSGKTNTIAWTSHRLSSLHDEENNAIFDSIIVVTDR
                     KVLDKQLQNAIYQLEHKNGLVVKIDEDKNSSDLADAIVNRAKIIITTIQKFQYALPKI
                     EQLDKRRYAVIIDEAHSSTSGENMNALKETLAGKTLEEAKVIDAENEKNEKSSIDKMN
                     ELIEKRTNLGNISFFAFTATPKNKTMQVFGRIGEDGLPHEFHLYSMKQAIEEGFILDV
                     LKNFMPISVYYKVGKKIIDNPEFDKGEAKRAINKFVSLNEHNIRQKVETIVDDFVDNR
                     SMWLEGKAKGMIVTASRLHAVRYKLAIDQYIKEKGYDMKALVAFSGTVKDGDDEYTEV
                     GMNKEVAPDITDSNLPNIFDKNEFKLLIVAEKYQTGFDQPKLCAMYVDKKLDGVKTVQ
                     TLSRLNRTYPNKQTFILDFQNSVEDIQDAYKPYFEMTNIDKVTDPNVVNDLWYQLHEY
                     AVYTDEEVNDFTVLFYKEKRTSSQDAKMNNLIDKAVERYCYLEEDDEKKSDEFKKKCQ
                     KYITFYNFLIQIYPIKNLNLLRLQVYLVALLKKLPKKSKQRIDLDDMLSLDYYKLQKL
                     GLDDKKGTDISLYNGEGELVGISETATSRVSEADEEYLEDIIKKINDVFGVGLTEDDR
                     IMINTYEEMFKNDKDIMDIAKANSYEDLVSTFGKKYFKRGIVKTKNRNDRLVKEILTN
                     NNLQNYMIHYLAEKIYEEANR"
     gene            complement(9183..9353)
                     /locus_tag="PC545_001422"
     CDS             complement(9183..9353)
                     /locus_tag="PC545_001422"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456196.1"
                     /translation="MNNDKPFIEFEEEVDFILTECKKEGVSIDRDTIELILDLDLKFL
                     ELKGIATPLEDE"
     gene            9634..9969
                     /locus_tag="PC545_001423"
     CDS             9634..9969
                     /locus_tag="PC545_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456197.1"
                     /translation="MIKLWKKEDLNLLLEYPKEVVENVDNVINILDENYGCNRKLTDD
                     GGYVCIIEDIKEVENLKSNILKGLVEEFSDVIYEDEVNTYNSTLYLLSSDYSVTVISK
                     NEETEYLLK"
     gene            10033..10491
                     /locus_tag="PC545_001424"
                     /pseudo
     CDS             10033..10491
                     /locus_tag="PC545_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            10631..10924
                     /locus_tag="PC545_001425"
     CDS             10631..10924
                     /locus_tag="PC545_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456198.1"
                     /translation="MFKKENRYMTKEIAENLPIEIAILLWNLIDDLTTEKDYLQLFEI
                     NPIGLGVVEIVHKQEVPEYEARIYIQNDKIKDKLKIYVIDNVEYRTMMFSNEY"
     gene            10951..11547
                     /locus_tag="PC545_001426"
     CDS             10951..11547
                     /locus_tag="PC545_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456199.1"
                     /translation="MINNKSEIINKLFFNELKELIDKYNNIDDKTVDVIEKIFTCIED
                     EEIKNYLIRDNCKLQELVNTYNKLEDLNIDEAIFFAWYNLTINTISIDKASEYYDDLT
                     TSNYIEVENHIIYTNERDLRKYAEDELETMLSMEHHIDRLLHKDMLIDMWLNNTSKEE
                     VIEEILMSDDIEDILNLSPEYAFTLANGIEYRYSEKEE"
     gene            11700..11969
                     /locus_tag="PC545_001427"
     CDS             11700..11969
                     /locus_tag="PC545_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HDF2456200.1"
                     /translation="MTNNNNNCDYKGIYADMVEVLGEEIVIKLHKYYRGQQITFPMKL
                     YSIEYVERYIVKNYRTKTIKEMCRELGYTEGWIKQLINKNRLNSK"
     gene            11995..12237
                     /locus_tag="PC545_001428"
     CDS             11995..12237
                     /locus_tag="PC545_001428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456201.1"
                     /translation="MILTVLAILMGTGIFFLINSMLDITYFGCGPIGFLWFGCFIVSY
                     AILTSLGGIVLGLLKWIVIGLIILFVIGLIANKIND"
     gene            12443..13576
                     /locus_tag="PC545_001429"
     CDS             12443..13576
                     /locus_tag="PC545_001429"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012938.1,HMM:NF016983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2456202.1"
                     /translation="MQDFDFISETIKKFQSDLENMDKVNILVVGKTGVGKSTLINNIF
                     REELAETGIGRPVTQHLKKISKEGLPINLYDTKGLELEEDVQETIKKEILDEIENCNR
                     NSLERNDKSELMHVCWYCINTSVARCEETDIKWINELSEKIPVIVVLTQGFPRKKARE
                     LKSSIDDLNLNCKNVIPVLAEQLVEEEDEIVIKSYGLDKLVEVTYQVIPKEVKKAFVN
                     VQKVDIKKKVEESRKWVLGYVGGSGAIGFSPIPFSDAPLLATAQVAMISHITVIFGLQ
                     VDKALLTAIVSSIGGVTSATLLGKTLVSNILKFIPGAGTIAGGAISGSVAALTTTALG
                     MAYISVLEYILNENMKGNEVNNTEIAEKMKDAYKDQLGMLSKK"
     gene            14340..14585
                     /locus_tag="PC545_001430"
     CDS             14340..14585
                     /locus_tag="PC545_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456203.1"
                     /translation="MRSVTVEDGDERFAIEKIYIKSLQRYEVRICLYRDCRDRINKLI
                     PRPVDLTMDKFVALILVGLKAGVLDDDFKQELIKGLA"
     gene            14933..16294
                     /locus_tag="PC545_001431"
     CDS             14933..16294
                     /locus_tag="PC545_001431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456204.1"
                     /translation="MKGNINKKDIDLSKITFDKFLEDEGLQNRERDYLYVDDYDNTLD
                     TQEFKLHIIEENTDNTYIDKDKYWYLDLDDSLFEYEEDEDTIAKTLYSLIDIRIKFLK
                     HVEYRKYINLGVIDEEYFDEIKSFLKFYIVAKTKKNELGNSNLKQMAIDIFDKERFED
                     DISDDDMENISEWIGEDQNFKMFYDKVCRDLSNSTQDKEILKLAGIRNNKVKHYKGRK
                     ISRKCKLELKSLTKMWEYETKLTHDANKAHKKIKSNMLEKMTDKNYKNIYEYLNNEII
                     NSNEEYKNIYFYSLELKLANELRKCTLKNLNKIKDIENQVKFISYTIAYIHIVPVLTI
                     REKLSDKLFNILSTNEGKDIEKFTKEVSLIMRIINIECLELLNIVSKNNYYKNFSEKE
                     LEEFKSIYNINNGRKSFENKYKIKVDYNIDDVRKYGEVIKAINIESKKKIVDRIKKKR
                     KSN"
     gene            16484..16801
                     /locus_tag="PC545_001432"
     CDS             16484..16801
                     /locus_tag="PC545_001432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456205.1"
                     /translation="MTNFKSVKVELILLIECEEGNRNELEGMIDEGVLNEKEYSLTIL
                     GSTEYEDNARAIYILMNTGGSYEKNLQRLSRLHLKIENLLKDTNVKYRGISLVPNIVK
                     WDK"
     gene            16813..18576
                     /locus_tag="PC545_001433"
     CDS             16813..18576
                     /locus_tag="PC545_001433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456206.1"
                     /translation="MSKAINREELIKLYEEIGLNVLDNNTVMNSSNKKICNFLVNIIE
                     QRVIDGKMYLCINAMGINGELLSEVILDSNTINRTTWVIENWGFKYSIDKKYINDFIE
                     LIQHLCMNIKTKYIYDHTGWIYQDNKWIYLHGGGVIGGSDVQVELDESIDNYILPDKI
                     SDINKACEMSLKLVDLIEDDKGIIFSSLVYLSPLIEIISRKIKPPENIVWTYGKTGSR
                     KTAVSKVVLSHFGDFNKKIPATFNDTITAIELKANKLKDSLYLCDDFAPKQDFRDKKI
                     QDSKAESIIRMYGDRVGKGRSSNKLDIKDAKRPRGMILITGEDVICGESSNARLLSIE
                     LNRSSVDLNILTELQNNSHLLGEAMRGYIEWLLSEMGNIDELSNILLYNFKSYRDEIR
                     EQYKDDIHGRTIESSAWILVGFSCMLNYIRDKGVIDKYEDDEYMDRAIKVVERLIEEQ
                     IKLISRNSPIDIFLNTLKESINSKSIRIATLNDQNQVIEDKLEYICGYKDKEYYYFYT
                     DKVYSFIEFENKKRGIHLGISQRQLVKQLRDSGIVKVDSNNDSPKKTIHTKDASDSTG
                     YTTIRPRMLQIERSIIENFQI"
     gene            18639..18794
                     /locus_tag="PC545_001434"
     CDS             18639..18794
                     /locus_tag="PC545_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456207.1"
                     /translation="MTETYKISIGRKDLYSKDYKVVERTDYEKRHTVNISRSLVDILS
                     ILVKEDR"
     gene            18881..20446
                     /locus_tag="PC545_001435"
     CDS             18881..20446
                     /locus_tag="PC545_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2456208.1"
                     /translation="MTGKKAVVYARFSSDNQRDESIDAQLRAINEYADKNNIKIVNQF
                     IDRAKSATSDKRPEFQNMIKFCEADTTGISMVIVHKLDRFSRDKYDSAMYKQKLKVKG
                     IRVVSVLENLDNSPESLILESVIEGMAQYYSANLAREVAKGQKENALKALHNGGDAPL
                     GYDVAHDKTYTVNEEEAQAVKIIFDRYIKGYSYSKIIDELNILGYKTKRGNKFGKNSL
                     HGILNNEKYTGVYVFNKTQRKGVDGKRNGHKQKSEEEIIKIEGGMPQIIDKGVFKQAQ
                     DMIQKRKKTPGAHKATTTYLLTGLIKCGECGHAFQGNKRKDSYGNEYVSYRCGCRKQK
                     RECNNREIKRDYLEEFILQELEKNILNDEAIPILSKALNEKLNDKNESNAELLKNLEQ
                     KLDKVNKEISNILNAIMNGIVNSMLKDKLDELEQVKFNLDLKMNELKIENKVVDGVGV
                     TEDQIRGMFSRFKEFVLERNIPECKKFIGDYIKEVIVYKDHIEVIFNVVFCFVDNDIT
                     HDLHIITERGKIT"
     gene            20592..21884
                     /locus_tag="PC545_001436"
     CDS             20592..21884
                     /locus_tag="PC545_001436"
                     /inference="COORDINATES: protein motif:HMM:NF014177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2456209.1"
                     /translation="MADIFTVLNYQGSKKRLLDFIYENTQEYIDSDKAFLDICSGTSA
                     VAYNMKRHFTVYTNDSENYAYQISNSIVKNNERYNFSQSDEMIIREKYNINLQRLNNI
                     FNDMVNQEDMLLEQEDADGLIELYSNCPCIWKNNVDIEIEGNTIQSIYDLRKCKRDIP
                     NVLFTLYYSSSYFGIKQSMEIDSIRYAIEFIESEITKSILFTSLFFAMKECVFAKDGH
                     MAQPLNMETGKKRMLRLKARSIYDIFIDKLKEFNTEEFVLSQKENKCFNKDFRTLLET
                     EESIKDEVGFIYIDPPYTDMQYSRYYHILNTVVLYDYPEISLSRGTISKGIYREGRYQ
                     SPLSQRKHALNEIDFLFSFCKENKINVALSYAYPIDTEKQATNRYTASIDDLINVAKK
                     HFGDNVKVVHEEYSHANNRNSDKKNVLEYLIMGIHNKE"
     gene            21887..23506
                     /locus_tag="PC545_001437"
     CDS             21887..23506
                     /locus_tag="PC545_001437"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDF2456210.1"
                     /translation="MDKINKLKERIANIEATNKNDIYSSNLYWSQKPFNICDEIIEHL
                     SEDGDIIFDPFMGSGVTVIEALKKEYKRNAIGCDINDAPIFIVKTLLEKYNVPKIEKD
                     IKELNNKLSKLSTYYSTKCRECGEVVTISKTIFDKENRNAKCELKSIHYKCKECKNDF
                     KGEPNETEISKFFIDEELKNIDDFQFIANSRLAVGEGDTVRTIFSNRNLIVLDKILGH
                     IDEYKNSESYEPMKYILLSILHLSKITDTHSNSQWPLWIPKKDCVEKNVVELFIKNSK
                     KTIKALKLASKYLCENRENVELIKNLDRGKFKLIKDGVQNIEDDVIPNEFVDLIITDP
                     PYMGQVLYSEYMQLYKPFMDLEFELENEIVISSAPARDKGKVLYFDMMNSAFENISRV
                     LKVGKYMCMYFHDSNLDVWDNIIDIMTNNNLKYMGQIHVSKNKNTLKNILSPKKSLNG
                     DCIIFFKKDLNINYDINEVDNIEDKINNIAQAIIDIKGYASTPELYDNGALEYIISNG
                     KLKEISKKYKDLTSIFEQRFNWDKERGVWTV"
     gene            complement(23631..24782)
                     /locus_tag="PC545_001438"
     CDS             complement(23631..24782)
                     /locus_tag="PC545_001438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456211.1"
                     /translation="MKKLTIRLKDDFHKDLLDLVAFKNDSINSYVINLIKNDLSKNTN
                     LLRSYDNMIRDNITNVLITQKINELKAIQDGIFKEYKVNDILSNSKIYEVMTANSLNH
                     ILIPGHSGSRDAKDEFGNEFEYKHFKKSSSNHSWTFNDFSDTSIAKLKKAKAVIFAHI
                     NDVDFPYPGKLDWYYSIPGDIMCEYLLKYTQSIQNSRKMINVSPNQLESRLNASKIIP
                     SAQNGVYDKYLSAIYKASSELEELTGVKNLLTSNKFWELIVALELNHQVNPEQGGREG
                     AHDAYDSEGNPYEYKVSKNHAWNFQDISDNVLNKYYNDKAIILAVVDKENIEVEHIYS
                     TEPADVVPLLRKKLQEKINRCLEKGSPIRRLQVSLSKSDLTRVNAKKLY"
     gene            25288..25503
                     /locus_tag="PC545_001439"
     CDS             25288..25503
                     /locus_tag="PC545_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HDF2456212.1"
                     /translation="MDERKLEFSIFCIEALAEKLNIDSEGVYKLLKHDTDILDNYIIP
                     CYEALHSQSKGYIVEDLMQVLKEKGVI"
     gene            25503..25982
                     /locus_tag="PC545_001440"
     CDS             25503..25982
                     /locus_tag="PC545_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HDF2456213.1"
                     /translation="MIVYHGSYCVVKTPSITFSRDSLDFGKGFYVTSIKTQAINWTER
                     FKKRGKKAYLNSYSLDIDKLKENCKVKIFDSYNLEWLDFIIECRNMSDIYMKYDIIIG
                     GIADDKIYNTIELYQDNLIEKDEALKRLKYYKPNEQICITNQNVIDKYLKFRYEEEV"
     gene            25988..26218
                     /locus_tag="PC545_001441"
     CDS             25988..26218
                     /locus_tag="PC545_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456214.1"
                     /translation="MEANKTLLQILYKKIILEFSKQTGKSLEESMDYLYTSETYKLIS
                     EGVSDMHCKGHIYLAQELMLENGLMYHKGYPR"
     gene            complement(26334..27500)
                     /locus_tag="PC545_001442"
     CDS             complement(26334..27500)
                     /locus_tag="PC545_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2456215.1"
                     /translation="MATKRANGEGSIGKYKDGWRSRIMIGYNEDGKPLRKEFYGKTQK
                     EVKDKLDAYKKQYYLSSDISDDKITLEHWFYNWLFEYRIKDLKPKSFERYESIYRNYI
                     KDTDLGNVKLKYLRVSHIQKYYNRLLDSNKSIPTIRQINTKLKTCLSEAEKQGLIQRN
                     YCTMVNIPVEKKENKLEILTLEQQKSFIKAIDGHKLEVLFLLALGTGLRLGELLGLKW
                     FDIDFKKSNLTVKRTLQRTYFIDKTGNRELKVLEQGPKTSNSYRTVPIPKDVLNKLKE
                     HKVNQNTDILKAGELYINNNYVFCNELGIPIDDKRPLRNLKSILNSLNIEPIKFHGLR
                     KTYATRLFENDVPPKTVQVLMGHYDISITLNIYTQVMEDKKVEAVEKLDKIFSL"
     gene            complement(27490..28242)
                     /locus_tag="PC545_001443"
     CDS             complement(27490..28242)
                     /locus_tag="PC545_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2456216.1"
                     /translation="MNKSYIPKYTHKFPTNIPHGDKKYTLTYIRRMISEFVYDMGNLE
                     NNPFTFPEVQTLLDGITVGGHKLSDQNQILNIKSSWDYVIKLSNKENLSLNKDTICSI
                     HKIVAKDEALIVGDFRNGNIGIAGTTQYECIEATLLNDLFVNDINIINKITNPLEKAI
                     IINLWLSYCQFFYDGNKRTARLTSNLILINNDIGVLSIPAKHKQEYNTLMLNFYETLE
                     ADEVIKFLLEKCITFFDGYNYKSYKELFKNGN"
     gene            complement(28334..28825)
                     /locus_tag="PC545_001444"
     CDS             complement(28334..28825)
                     /locus_tag="PC545_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456217.1"
                     /translation="MMNNTIGEKIKQYRKSINMSRDELANKIGCSVHAIAKYEQNQRM
                     PSLDMIRKIATALSIAINKLIVEENDLDTLTNNNINLKVFDKELDLVIHILENYGYDI
                     KTDYKKKFEITDNADTIKPINLNFSDFKDFSKNLNWVINGLVENFIQENSNGLIEDED
                     EEY"
     gene            complement(28822..29020)
                     /locus_tag="PC545_001445"
                     /pseudo
     CDS             complement(28822..29020)
                     /locus_tag="PC545_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729178.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29077..29838)
                     /locus_tag="PC545_001446"
     CDS             complement(29077..29838)
                     /locus_tag="PC545_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456218.1"
                     /translation="MQIGKNLKKIRKQKELTQIQLAEISGISRNALINYENDKRIPSI
                     DTLSKLAKALKIEKTIFYFDIENEVMTNEDVLYLLQSLFPEEYYSFDEFLNAISNLME
                     DDIRITSSLLLGAWSDKDQYYKLGEKLELNENQVYNWILSDCISNTELDFSLHPDDIK
                     EIIKNDVLTKQSIDELLDIGLSEESIETIYDYFSDSTDNGSNIRLKKLISQKENITIK
                     NNDYFKLLQENNELKEKNEKLENTLKEIKKLLTKI"
     gene            29985..30185
                     /locus_tag="PC545_001447"
     CDS             29985..30185
                     /locus_tag="PC545_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456219.1"
                     /translation="MGINLKIFRIKKGLKQQELADRVGISRYYLSNLETGKANNPSND
                     LMIKLSRALDTTVEELFFSKED"
     gene            30189..30503
                     /locus_tag="PC545_001448"
     CDS             30189..30503
                     /locus_tag="PC545_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456220.1"
                     /translation="MKENNNLEVLKTLQATLNIIVKNEEEAQEKKQGYKIIENAKRNK
                     AELITCKEASKLYPIGESKFRQLCHSKNKGFPCIWINNRVYIIKNKLDDWFIENANGI
                     KL"
     gene            30525..31268
                     /locus_tag="PC545_001449"
     CDS             30525..31268
                     /locus_tag="PC545_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456221.1"
                     /translation="MSNKGWIKLYRELLDKSIWKDIKPERKVILITLLLMASHKENHV
                     FIDNKKIKIKFGQFISSYSKIAERCGKGVSYQMVRSCLLVFKNAGFLTYETTNKYIVI
                     TIENWELYQENIENQQPNQQTINNQSTTIKNIKNENNIIYSEIISKFNSTCIELSKVK
                     KLTDTRKRRINSRIKEYDKETVLEVINIVSKTSFLNGNNNTGWKANFDWIMNSSNFTK
                     ILEGNYNSKEIVINKETGIYNPDDSQLKW"
     gene            complement(31974..33887)
                     /locus_tag="PC545_001450"
     CDS             complement(31974..33887)
                     /locus_tag="PC545_001450"
                     /inference="COORDINATES: protein motif:HMM:NF020926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HDF2456222.1"
                     /translation="MSIKLDDKTHNLRINQATSVFGPGAMLDFIDQTLMTAHPNYWTN
                     TIKIQDDRLQATLRVNELRTPPTIDDRSSVPFVRFPRWYFCPKCRKFMPIEDWEKLYK
                     NAHKDKPMKIPICLTCSTKKSTKQLVPTGIITICEHGHIDDFPWVKWVHFRNKSGRKE
                     ICENPQILMKTGKSNLGLEGIELECSCGAKANMKGAFMRLKSSKSNDYGVPSEYKEMF
                     KCSGNSPWYNKKVNKCTKYSFASQRGASNVYYPKIESSIIIPPYSDTLNSKVLSSKGY
                     DTFKKFLDRAIKKNKLERFMDEDFEDFASDIAKETGFDTDKVSIVLKDLLPFEDSSPS
                     TTTRNQYREEEYEALIGNIDAKSKKTNDFTIEEQNLSDYDIPNFSKIVLVKRLREVRA
                     LTGFSRVNPPDNNIFGEEDIDDSCNDTKVISLRSRDCKKSFYPASEVRGEGIFIEINN
                     DMVKSWISSNPKIQDNASILNSRYNKDINVNDKAYRKITPKFLLLHTLAHLIIRELSF
                     VCGYATSSLRERIYCNNSENEKVMSGILVYTASGDSEGTLGGLVRQGMHDSLPSILNS
                     ALNRALWCSNDPICIDSTGQGRNSLNLGACHSCTLLPETSCEEYNLLLDRSMLIGDMD
                     NRDIGFFKNLILI"
     gene            complement(33889..37416)
                     /locus_tag="PC545_001451"
     CDS             complement(33889..37416)
                     /locus_tag="PC545_001451"
                     /inference="COORDINATES: protein motif:HMM:NF012493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDF2456223.1"
                     /translation="MNCSELRDKTLDLVRKELLGPGSEDLCENIKYEIITDSPIERYS
                     TGILYPLNNNIDVSNTSEYDGEDNVFTSDDTQNELDRAISMSNETLPSSMGMTFFLKK
                     DAEVINFRVNYATYRNALYTDCHVKYTGDDFYSSNTINEYVELRGSFLYLKQKLTSEK
                     VYNLLKKHDLLDNAEAKFVLYKLANQCPDDENKTGGYVRVPHENLIVSIDMDKNYIKK
                     PISKDLDIVLSKRIYSNDFVSITCMLVNSQSGPKKYNNTIFQPELMVLAEDNNDLEFI
                     DIFTAGSVNTFKKLDSEEISLDLLYRNKKTFATGHGVAIDQDIQNDALIKLCTEFIPH
                     FEVPKLDFNVDELSDVSRELLSMKNLSDISDLSKDKKIYFLYKFVDAYDIWIDDLEED
                     SKDLSDVFKDVAYEHIKNCRDTKSRMLEGINLIKSNDEVYRAFSLMNRAILMQRCHSI
                     HITDRLPGSNNLDYIDYSNDNSAWRPFQLAYILTCLSSIENPDSKHRDLVDLLWIPTG
                     GGKTEAYLGLSAFTIFLRRIRNKENGNGTAIIMRYTLRLLAAQQFIRASILICACEII
                     RKENKSELGKKEISIGLWIGSDQTPNFNDEAEEMLNNLTGNNIKFRSSINSEYELDNL
                     KSKYNKFQLLKCPWCGTKLVKEWDKTSKKAVGDWGYRIKSRPKKFYLCCPEEDCSFED
                     MLPIQVVDEEIYNNPPTLLFGTVDKFAMITWTTEVASLFALDDDNSNLSPELIIQDEL
                     HLISGPLGTMVGLYETALDAMCSYKGIKPKIIASTATIRKAQNQVKNLYNRNVAQFPP
                     PGLTSSNSFFVKEMPLSKKRGRLYVGVIPSGKTQVTVEVRLMSALLQRLKMMENVELN
                     LLDKYWTLVSYFNSIRELGKCSTLLNDDIKDNMERLCKRVSAHPYQSLRNLNEQRELT
                     SRLTSTKINTTLDELSNTLTSNNLTVDTLLASNMISVGVDVDRLNLMVVVGQPKLTSE
                     YIQASSRVGRSNEGLIFTLYNPTKTRDRSHYEQFFSYHQSFYKFVEPTSITPFSEPAR
                     ERGMHAVLISMIRHILGLQANDLASRFSKNMDGVEDLKNFIIDRSIDIFRENNQNADD
                     NIVNEFKSEISSELDIIMDLWHDKAQISDKDNLLHYYKRPSKFDNKIYNNLLIPYDKD
                     VITNEKQTLNSMRNVDKQCTVEIIELEEN"
     gene            complement(37434..40052)
                     /locus_tag="PC545_001452"
     CDS             complement(37434..40052)
                     /locus_tag="PC545_001452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456224.1"
                     /translation="MLTTNIHYILDNDICEIYFNKQRLTNSKYYQDNINVLNYIINEK
                     NNNLIINYNDKYIYAIYLLNIAINSYLNNTLNPNNHILTKLDKGMPLLYKGQIVIFKN
                     INNTHITLGHKNKTDTTLIPIDSAYMLITYNGHLEVNSMGKTPASKTFQTKNVLSSLG
                     FSDLKNMTGVINDSTLIILPCKDDISDLVSNIKIKDINNTYKFTELFPCSYISSTGAE
                     TDYPGNHAKQIPLLKFTTNISSAFEIIKSDKSIKNVFIIGDYPIYKHINDFERILNRK
                     RIESVNIVTSNSNISNILDLPNIDNLNIYSWSKDVLLTYSQDFENTSTFQKLWLDKLI
                     NKNICTTSVDSNISDLIHQARKSLYYISKYDFDISIKNSLMMCSYSLLKILENTPFNL
                     DFLEETIKLLNLNIATPSAKLENIKYLISSTSPDLELSALFDDVIVKFERIINELSDN
                     NTKFNTLNKLVSSYKYKTFTPNTPVILLQKQYEAIILKNLLKSRNINLQVISAEKFKD
                     SKTIKNLIMFGFYDLKYLDILNSDLIINITLYLYNSELSKYNYYMNQINSARNKIEKN
                     NKIYNLLDIKPFKPTYETNKLEAYSTDSPISDDDSMDEYISNYKFDINLNEIILSLSI
                     NQGIYNSKKEHVTSFMISDNNEYIFCTKKAIAYSIDWDKKSVRKKKLSEAKVNDWFLF
                     VDDYLNEEGELVINVIQKLLELNLLPSHYLDDYKLTNSWKSELKNYISYSKYNYNDVS
                     NSLAVLGEKINPITIKNWVTNKRLIGPIYLKTFEKIFKVIDSKLDHTEVYDATKSIRS
                     LHTKIKKSIDTFVSDNFFSSKSDIIKDEVSAVVLSMLGNTNDYVTPVQIREIHSCDKY
                     LSPHLVNKVLDLYSVY"
BASE COUNT        15905 a         4434 c         6293 g        13448 t
ORIGIN      
        1 tataatatgt aatatttttg ttttgaaatt tataaattag tattactggt tagagttagt
       61 ttagtattaa gtatattcga agtagttttt tagctatttt catacattta tattacttaa
      121 ttatatatag cctaactaac ttaatatatc agtgaaatat tgactaaaat gcctaaatat
      181 gttatcatac aagttagtca aataatttgg caacattaaa tatttagtaa atttctaaca
      241 tttatggaac aaatttctag caaagaaggt gagatttata tggctggtaa aaaaatatta
      301 gcaacaaaca aaagagcaag acatgaatat tttatagaag aaacttatga atgtggaatt
      361 gaattaaaag gaacagaagt aaaatcaata agacaaggca aagttaacct aagtgatgga
      421 tttgcatctg tagataatag cgaagtattt ttaaaacaag ttcatataag tccatatgag
      481 caaggaaata tattcaatgt tgattcttta agagttagaa aactattact tcataagcat
      541 gaaataagaa agcttattgg agcaactact ataaaggggt attcacttat accacttagt
      601 ttgtatctaa aaaacggtaa agttaaagta gagattgcct tagcaaaagg taaaaaacta
      661 tatgataaac gtcaagatat ggctaagaaa gatgcacaaa gacgtataga aagagaaatg
      721 acaggtaagt attaaactag ataaaactgt attttttaga ataaaattct ttattatcta
      781 gaacataaca ttgattttta ttcttaaaag tattatatta tatatacatt aaaaatttaa
      841 taaaaatggt gataaagttt gagaaaagtc tactggacta taaactactc aagggggcgt
      901 aaaggtttcg acgtgggttt ggaacttgag gctgcatgtc gtgttactct gggtcacgta
      961 aaaactgggg aactttaaaa taaacgcaga cgataatttc gcgatagcag cctagttgct
     1021 gctcgtccct cttagtcctc ctgccgacta agactggacg tcattatgca gggaactact
     1081 ttaggggtgt ctcgacttga agtagattaa ttgggactgg ctaagcatag agcctgccac
     1141 ttggcgctat gtgaggtgag atattagtga agttggctaa gcatgtagat gcagcaagga
     1201 aaagatttgc ggacaggggt tcgactcccc tcgtctccac cattgaaatc cacgaaaaca
     1261 tattgatagt ttagtcaata tgtttttttt gtgccttgaa atgatataat tataatataa
     1321 actaatgtaa aatttaacta aaaggagata gtataagtga gtatagataa taattttaac
     1381 catgttgcat ttctgtggaa tatagcagaa agtttaagag gtacatataa agaagaagac
     1441 tatagaaaag taatgttacc actaatagta ataagaagat ttgattgtct attagatgat
     1501 tatgatagag aaataataaa atcagtatac gaagaatacg acttcttacc agaagaagaa
     1561 agagatgagc tagtaatagt agacttaaaa gaaaatcacg acataaactt acaattttac
     1621 aatgtaagtg attttacatg gaaaaaacta ttagatgata gtgaaaatat aaaatcaaac
     1681 tttgaagaat atctaaacgg attctcaaac aatgtaaaag aaataatagg taaatttaaa
     1741 tttaaagatg aaataaatca attagataaa aaagacaagc tatatgcagt tcttagcaaa
     1801 atgtatgaag tagatctaca tataaactca gtatctaaca ataaaatggg atacatatac
     1861 gaagaaatgt taagaagatt tacagaaaac tcagcagcag gagagcaata cacaccaaga
     1921 gaagttataa gactttgtat ggaaatacta tttatgggta aagaaaactt ccttacagaa
     1981 gaaggaaaag taatatctat agcagacttt tgttgtggta ctggaggaat gctttcaata
     2041 gctgaagact atgtagaaaa aataaatcca agtgcaatag taaatgtata tggacaagaa
     2101 ctacttgatg aatcattcgc aatatgtcag gcagatatgc taatgaaagg gcaaaatcca
     2161 gataacataa gacttggaaa tactttaaca caagatagat ttaacggaga aaaaataaga
     2221 tttttaatat caaatccgcc ttttggtgta acttggaaag acgaagaaaa gaaagtaaaa
     2281 gaagaagcag acttaggttt tgatggaaga tttggagcag gtactccaag agtaagtgat
     2341 ggttcattac ttttcctaca aaatatgata agtaaaatgt atgatgatga agaaggaagt
     2401 agaatagcta taatatttaa cggaagtcca ttatttacag gagatgcagg aagtggagaa
     2461 tcaaacatta gaaaatggat aatagaaaat gatttactag aaggtataat agcactacct
     2521 actgatatgt tctacaatac aggaatagct acatatattt gggtacttac taacaaaaaa
     2581 gaagctaaaa gaaaaggtaa gatacaactt gtaaatgcta gtgaatatta tcaattaatg
     2641 agaaaaagct taggaaacaa aagaaaagaa ataagtcttg aacaaataga acaaataaaa
     2701 gaaatatatg aaaaatttga agaaagtgaa aatagtaaaa tatttgataa cgaagatttt
     2761 ggatatagaa aagtaacaat agaaagacct ttaaaactaa gctttaaagt aaacgaagaa
     2821 gctatagaaa atgtaaaaaa tactactcaa tttataaatc ttgcagtttc taagaaaaaa
     2881 gatatagaag ttaaagaaaa agaagaagct gaaggaaaaa taaaacaaga taagttaata
     2941 aaacttttag aaagctttga caatacttta gagtatatgg acagggataa atttataaaa
     3001 gatttaaaag ctaaatcaaa agagtttgat atacaacttt cagcaggact tataaaggct
     3061 atagttaata ctattggtgt aagaaatgaa gatgcagaag tttgtaaaga tagcaaaggt
     3121 aatatagaaa gtgatagttc attaaaagat actgaaagta taggtcttaa agatgatgta
     3181 tatgagtatt ttgaaaagga agtaaagcct catgttaatg atgcttatat agatgaaagt
     3241 agtataaata atataggata tgagatacca tttactagac atttttataa gtatgaagag
     3301 ttaagaagtt ttgctgagat tatgaaggaa gtagaaagtt tagaaagtga gatagcacta
     3361 aagataagga aggtgctagg gtaatgggat acagatatag aagtgatgat gagttaaagg
     3421 atagtggagt tgagtggtta gggaagattc ctaaggattg ggaagtcaga caattaaaaa
     3481 gggtactatc agatagaaaa gaaaataatg aacctataaa aacaagtaat atactgtcat
     3541 tgacaataga aaagggagtt attccatatt ctgaaaaagg tactggtggt aataaagcta
     3601 aagaagattt aacacaatac aaattagtat atccaaatga tattgtatta aatagtatga
     3661 atgtaattgt tggtgcagta ggactatcaa aatattttgg gtgtgcgagt cctgcatatt
     3721 atatgttata taaaataaat gctaacgatg atataagatt ttataattat atttttaaat
     3781 ctaaattatt ccaggatagt ttaaaaggat taggtaatgg aataatgatg aaacaatcag
     3841 aaacttcagg taagttaaat acgatcagaa tgagaattcc aatggaaaaa ctaagttcag
     3901 taaagatacc aattgctttt aacttagatc aagaaaaaat agcaaacttt cttgatgaaa
     3961 agacttcaca gtttgattca attatatcta agaaagaaaa gttaatagaa agattagaag
     4021 aagcaaagaa aagccttata agtgaggttg ttactggtaa ggttaaggtt gttaagactg
     4081 atgatgggta tgagcttata aagagaagta gtgaagagat gaaggatagt ggagttgagt
     4141 ggttaggtga gatacctaag gaatgggata taaaaagact tagattttta ggaaaactac
     4201 aaaacggtat aagtaaaagt agtgattcat tcggttatgg ttatccattt gttagttata
     4261 gtaatgtata tagaaatata gaattaccac atatagtaga aggtctagta aattcaactg
     4321 aagaagaacg taaaatttat tcagtagaag aaggagatgt attctttaca agaacttcag
     4381 aaacagtaga agaaattggt tttgcttcta cgtgtatgaa aactattgat aattctactt
     4441 ttgcaggatt tttaataagg tttagaccga atgataaaag actatacaaa ggattttcta
     4501 aatattactt tagatgtgat ttaaatagaa aatattttgt aaaagagatg aatttagtaa
     4561 ctagagcatc tttatctcaa aatcttttaa ataatctttc tgttgtattg cctaagtatg
     4621 aagaacaagt tgagatatat aatgcacttg aattaaaagt tagctacatt aatttatcga
     4681 ttaataagtt aaagtaccaa atagaaaaac taaaagaagc taaacaaagt ttaataagtg
     4741 aagcagtaac aggaaaaata gagatactag attaacttct agtatcttaa tatagatata
     4801 gattatacat acaaagttaa ataaaaggga gaattaatgg ataaaaatat atttcaaatt
     4861 aatataaata cagatgtact gataagatta aggaatcaaa taaatgaaga atattatata
     4921 tcttatagaa aagaatttta tgataaaaat aataaaaaac atcgcgcttg ggataaaata
     4981 tgtgcaataa tggataggct tgacgatact gtagattatt taaataaact caaattaaat
     5041 acaggtaaat atagacgaag tgcatttgac ttctttgaat ttatgaatca ggcttccgta
     5101 gtaatggatt gtataacaca tcttgcaaaa atatttgaag taccatatga agaagttgaa
     5161 acttcaactg aaatttttaa tgagcttgga aaagagggta aaggtacaga taaaaaatac
     5221 tttgaatatc tacgttcatt gtgttcagtc catccaatgg aaactagtag acacaaaata
     5281 taccaagaaa atagttttga atgtagtcct tatgtagttt ggaatgataa aagtatatgg
     5341 actaatgatg ctagcgattt atatgcagtg gtatacacaa gtaaagataa tgatagtttc
     5401 aaaagaattc atatatatat gaatcaaata tttgagtatg taaaaagaag agtaaatttt
     5461 attgaaaata taatagaaca tatagaaaag tatcaacaag aaataattga aatttttagg
     5521 aatactccaa taaagaaaga agaagatttt gaaagttata tagattattt aagaaatcta
     5581 aaaaaagaga tagaaataag atatggtaat gattcttttc atgaattaaa ctaccttata
     5641 gaattatttg atttgccatt atcaaatcct gataatgaag agaagatgaa cttatataaa
     5701 aatgctttaa aatatgctat caaatttgag cataatagta ttcaaaatat gacaaatgaa
     5761 ggttataaga ataatggaat attatatcca caagtgaata taacaactac attactagaa
     5821 gagctaattt cattaaatag tggtagtgag gagagacgta agtacagata tgcaatagaa
     5881 aaaacatcat atctaagcta tgatagttgt tggagtgata gagaatgggc atatgatcaa
     5941 atagatgata tgatgccttt tttagaaaga tacgtaacat ttgataaagc tcaaagtgat
     6001 tttgagcatt atgtattggt aaaactagca ctttatttag agtgcttaga aaatgaatgt
     6061 ttaataaata agaacgttcc taatgataaa aagtttcgta ttaaataatt atagctaata
     6121 aaatagggag agggaggata ttatgttacc aaaagaaaaa cacctagaag aatctattga
     6181 aaatcattta gtttcaattg gatatgaaca aggtcattca caagaatttg accttgaaaa
     6241 ttgcttattt gtagatgatt tatttagatt cttagaaaat actcaaaaag aattactaga
     6301 tgagtttaaa tacaacaggg gaagtaatta caaaaaagct tttatagatt tattaaattc
     6361 aaacatcaaa agaagaggtc ttataagtgt acttagaaat ggtgtagaag atataatgat
     6421 gaacggaaaa tttcaattat tctacaacaa accaaatcta actactaata aaacagcgta
     6481 tgaaaattat aaacaaaata tattcaaaat aacaagacaa ttaaaatata gctcaaagca
     6541 caacaatact ttagatatag tattatcttt aaatggattt cccgttatag taatggaact
     6601 taaaaatcag tttactaatc aaaatgtgta taatgctata aagcaattca aaaaagatag
     6661 aaacccaaaa gaaactttat ttggatttaa tcaaagagtg ttagtatatt ttgcagtaga
     6721 tactgatgaa gtttatatga caacagagct aaaaggagat aaaacattct tcttaccgtt
     6781 taataaaggt aacaatagag gtaagggaaa tcctgtagta gaaggaaaag aaaaaacttc
     6841 ttatctttgg gaagaaatac ttactaaaga tagtttactt gatattataa aaagatttta
     6901 ctttattcaa gaagatgata aaggtaatca aagagcaata tttccaagat ttcatcaatt
     6961 agatgcagta aggaagatag tagaagattt aaagataaat aaagtaggta aaaattatct
     7021 tatccaacat agtgctggta gtggtaagac taacactata gcatggactt cacataggtt
     7081 atcaagtctt catgatgaag aaaataatgc tatatttgac tctataattg tagttacaga
     7141 cagaaaggta cttgataaac aattacaaaa tgctatatat caattagagc acaaaaacgg
     7201 acttgttgtt aagatagatg aagataaaaa ttcatcagac ttagcagatg ctattgtaaa
     7261 tagagcaaag attattataa ctactattca aaaattccag tatgcactac ctaagataga
     7321 acaacttgat aaaaggcgtt atgcagtaat tatagatgaa gcacattctt caacatctgg
     7381 tgaaaatatg aatgcattaa aagaaacttt agcaggaaag actttagaag aagctaaggt
     7441 aattgatgcg gaaaatgaaa agaatgaaaa gtctagtata gataagatga atgagcttat
     7501 agagaaaaga acaaatttag gaaatataag tttctttgct tttacagcta ctcctaagaa
     7561 taagactatg caagtatttg ggaggatagg tgaagatgga ctacctcatg aatttcattt
     7621 atattctatg aaacaagcga tagaggaagg atttatatta gatgtactaa aaaactttat
     7681 gcctataagt gtttattaca aagtaggaaa gaaaattatt gataaccctg agtttgataa
     7741 gggagaggct aagagggcta taaataagtt tgttagttta aatgagcaca atataaggca
     7801 aaaggttgaa actatagtag atgattttgt agataatagg tctatgtggt tagaaggtaa
     7861 agctaaaggt atgatagtta ctgcaagtag attacatgct gttagatata aactagctat
     7921 agaccaatat ataaaagaaa aaggatatga tatgaaagca cttgttgctt tttctggtac
     7981 tgtaaaagat ggtgatgatg agtatacaga ggtaggtatg aataaggaag tagcaccaga
     8041 tattactgat tcaaatttac ctaatatatt tgataaaaat gaatttaagc tattaatagt
     8101 tgcagaaaag tatcaaactg gatttgacca accaaaactt tgtgctatgt atgtggataa
     8161 aaaactagat ggtgttaaaa ctgttcagac tttatcaagg cttaatagaa cttatcctaa
     8221 taaacaaaca tttatattag atttccaaaa tagtgtggaa gacatacaag atgcatataa
     8281 gccatatttt gaaatgacta atattgataa ggtaacagat ccaaatgtag taaatgacct
     8341 ttggtatcag ttacatgaat atgcagtata tactgatgag gaagtaaatg attttacagt
     8401 attattctat aaagagaaaa gaacttcttc tcaagatgct aagatgaata atttaattga
     8461 taaggcagtt gaaaggtatt gttatttaga agaagatgat gaaaaaaaat ctgatgagtt
     8521 taagaaaaaa tgtcaaaagt atattacttt ttataatttt ttaattcaga tttatcctat
     8581 aaagaactta aatcttttaa gattgcaagt ttatttagta gcacttttaa agaagttacc
     8641 taaaaagagt aaacaaagaa tagaccttga tgatatgtta agccttgatt attataaatt
     8701 gcaaaagctt ggattagatg ataaaaaagg aacggatata tccttatata atggagaagg
     8761 tgagttagtt ggtatatctg aaactgcgac ttctcgtgta agtgaagctg atgaagagta
     8821 tttagaggat ataattaaaa aaataaatga tgtttttggt gtaggactta cggaagatga
     8881 tagaattatg ataaatactt atgaagagat gtttaagaat gacaaggata tcatggatat
     8941 agctaaagct aatagttatg aagatttagt tagcactttt ggtaagaagt attttaagag
     9001 aggtatagtt aaaaccaaaa atagaaatga tagattagtt aaggagatat taactaataa
     9061 taatttacaa aattatatga ttcattattt agctgaaaag atatatgaag aggcaaatag
     9121 ataagatacc tatagagagc taattaactc tccataggta ctttattatg agctattgta
     9181 tattattcat cttctaaagg tgtagcaatt ccttttaatt ctaaaaactt taagtctaaa
     9241 tcaagaataa gctctatagt atctctatct atagaaacac cttctttttt acactcagta
     9301 agtataaaat caacttcttc ttcaaactct ataaatggtt tatcgttatt catatttttg
     9361 ctcctttgat taaatataat gtatttactt taaattatat caaagaagag aaaaaaataa
     9421 cattatttgt aaaaaactat atatatataa tatatagttt taccatatat agataaactg
     9481 tttttaatta aattataaat atatattata ttaatgaatc tatattaaat agatagttga
     9541 gtaagtagta ataccaaaaa tattaaaaag agatgttggt agaaatacga acatctcttt
     9601 tttagtgcaa aaaattaaaa ggaggaagtt aatatgatta aattatggaa gaaagaagat
     9661 ttaaatttat tattagaata tccaaaggaa gtagttgaaa atgttgataa cgtcattaat
     9721 attcttgatg aaaactatgg atgtaacaga aaattaacag atgatggtgg atatgtgtgt
     9781 ataattgagg atattaaaga agtagaaaac ttaaaatcaa atatattaaa ggggttagta
     9841 gaagagttta gtgatgtaat atatgaagat gaagttaata catataattc aacactatac
     9901 ttgttatcta gtgattatag tgttactgta atttctaaaa atgaagaaac tgagtactta
     9961 cttaaataag catatacatt gataaggtgt atatgcttat tttaatttta ataaataaag
    10021 gagaaaagca gaatgaataa aaagagaact cctgccaaga gagttgatat agttttactt
    10081 aaattagtaa aagaacgtag tgttctatat gaaactagaa aaataagtaa tctttatgat
    10141 gcttatagat tagcaaagaa ttttttataa atagtgctag agaaaagttt gtagtggtat
    10201 gtttagatac aaagaattgg ccagtagcat agaggttgta agtataggca ctgtaaattt
    10261 atctatggta tatccaagag aaatatttaa agtagctata ttaaccaatt catctgaaat
    10321 gatatgcttt cacaatcacc caatgggtaa tactgacttt agcaaggaag atagttataa
    10381 ctaatatgct acagaagcgt ggagaaatat tatgcattga attagtagat acatatagtt
    10441 attgtagatg aggataaata ctttagcttt aaagaaaact gtaggatata agagtcttag
    10501 cttaaataaa ttaggctaag gctttttcta tgttccaaaa caattaaaaa taaatcaaat
    10561 ttaggttaag cccggaaagc ccttgaaaaa aataatacat ataaatttaa aactaaatag
    10621 gagttgtaac atgtttaaaa aagaaaatag gtatatgaca aaagagatag ctgaaaatct
    10681 accaattgaa atagctatac tactatggaa tttaatagat gacttaacta cagaaaaaga
    10741 ttatctacaa ctatttgaaa taaatcctat aggattagga gtagttgaga tagttcataa
    10801 gcaagaagta cctgaatatg aagctaggat atatatacag aatgataaaa ttaaagataa
    10861 attaaaaata tatgttatag ataatgttga atatagaaca atgatgtttt caaatgaata
    10921 ttagttaatg attaaaggag agagagtaat atgataaata ataaatctga aataattaat
    10981 aagttatttt tcaatgaatt aaaagagtta atagataaat acaacaatat agatgataaa
    11041 actgtagatg taatagaaaa gatatttaca tgtatagaag atgaagaaat aaagaattac
    11101 ctaataagag ataattgtaa gctacaagag ctagtaaata catacaataa acttgaagat
    11161 ttaaacatag atgaagctat attctttgct tggtacaact taactataaa tacaatatct
    11221 atagataaag caagtgagta ctacgatgat ttaactacaa gtaactatat agaagtagaa
    11281 aatcatataa tatatacaaa tgaaagagat ttaaggaagt atgctgaaga tgaattagaa
    11341 actatgttaa gcatggaaca tcatatagat agattactac ataaagacat gttaatagat
    11401 atgtggttaa acaatacttc aaaagaagaa gtaatagaag aaatattaat gagtgatgat
    11461 atagaagata tattgaattt atctccagaa tatgcattta cattagctaa tggaatagaa
    11521 tatagatact cagaaaaaga agaataaaat aagtatatgt actgaaaaat acgggcttta
    11581 agggcttata cattaaagta catatttgaa aataccatag attttacaag agaaaatcag
    11641 aatgaaactt tttcaaatac tagttattat taattagtgc tagaaaagga ggtggagtga
    11701 tgactaacaa caataataat tgtgattata agggcatata tgcagatatg gttgaggttt
    11761 tgggtgaaga aatagtcatt aaattacata agtattatcg tggacaacaa ataacatttc
    11821 caatgaaact atattctatt gaatatgtgg aacgatatat agtgaagaat tatagaacaa
    11881 aaacaataaa agaaatgtgt agagagttag gatatacaga gggatggata aagcaattaa
    11941 ttaataaaaa tagattaaat tcaaagtaat tagagaagga gattgacagt agatatgata
    12001 ttaacagtat tagcaatact tatggggaca ggtatatttt ttctgattaa ttcaatgcta
    12061 gatataacgt attttggatg tggcccaata ggatttttat ggtttggatg ttttattgtg
    12121 tcatatgcaa tattaacatc attaggtggt atagttttag gattgttaaa gtggatagta
    12181 ataggattga ttatactatt tgtaatagga ttaatagcga ataagataaa tgattaaatt
    12241 actgtagatt attttgataa aataattatt taatatattg aggcattaca tgatagaaga
    12301 ataaatataa atgacttaaa atctttaact taactaaaca aaaaatatat tatttattac
    12361 aaaaaatgac aaaaaaatta aatattatat tgtattatga caatataaaa cattggagtt
    12421 tataaattag gaggaatatt ttatgcaaga ttttgatttt ataagtgaga caataaagaa
    12481 atttcaatcc gatttagaaa acatggataa ggtaaatata cttgttgtag gtaaaacagg
    12541 agtaggtaaa agtacattaa taaataatat atttagagaa gaattagcag aaactggaat
    12601 tggtagacct gtaacacaac acttgaaaaa aataagcaaa gaaggattac caataaattt
    12661 atacgataca aagggattag agttagaaga agatgtgcaa gagactataa aaaaagagat
    12721 attagatgaa atagaaaatt gtaatagaaa ttcattagaa agaaatgata aaagtgagtt
    12781 aatgcatgta tgttggtatt gtataaatac atcagtagct agatgtgaag aaactgatat
    12841 aaaatggata aatgagttaa gtgaaaagat accagtaata gttgtattga cacaaggatt
    12901 cccaagaaaa aaagcaagag aattaaaatc tagtattgat gatttaaatt taaactgtaa
    12961 gaatgtaatt ccagttttgg cagagcaact agtagaagaa gaggatgaga tagttataaa
    13021 gtcatatggt ttagataaat tagtagaagt tacatatcaa gtaataccaa aagaggtcaa
    13081 gaaagctttt gttaatgtac aaaaggtaga tataaaaaaa aaggttgaag agtcgagaaa
    13141 atgggtttta ggatatgtag gaggatcagg agcaatagga ttttcaccaa taccattttc
    13201 agatgctcca ttattagcaa ccgcacaagt tgctatgata tctcatataa cagtaatatt
    13261 tgggttacaa gttgataaag ctttactaac ggcaattgta agttctatag gaggggttac
    13321 atcagctact ttactaggaa aaacattggt atcgaatata cttaagttta taccaggagc
    13381 tggaactata gcaggtggag cgataagtgg ttctgtagcg gcattaacaa ctacagcatt
    13441 aggaatggca tatataagtg tattagaata tatactaaat gagaatatga aaggtaatga
    13501 ggttaataat actgaaatag ctgaaaaaat gaaagatgca tacaaagatc aattaggaat
    13561 gttatctaag aaataattaa ttcaagataa agtataagat taattatagg gaaaaagtaa
    13621 aatttaaagg tatatttggt aaaacataaa caaaaatgat aatggcgtca atattatttg
    13681 ttgtcatatg tagtagctta gtatatatat tattgagtga taggtttaat cataagttaa
    13741 tttacttaga gaaagatata ttagatttaa gaaataaaaa tttgtaacta gctaataaag
    13801 gatcagtata gaaacagata aacaagagga taatgtagat attaaatcta aaaacactaa
    13861 taatgaagaa tataaaactt caaaactatt taggcactag atagagtatt tgctataatg
    13921 aataagctaa tagagaaatt aaaacgttta tagtaagata actaattcaa atacatttag
    13981 tggacttcaa ggaacatttg atacactatc aaactatggt agccaattga tagaccaatc
    14041 atctaaattg aatttatcaa atgttggatg gttagaaata tctgatataa ttacaagtat
    14101 aggaaaggac tattagattc tgaaaatatg tcatttgaag cattaaatat aatgggaaaa
    14161 ataggaaaat ttataaatta tattgaaatt aattatatta actaattaaa tattgataca
    14221 taatgccttg ctacagttaa atgtggcaag gcattatttt tgtaaagaaa ggaagaatta
    14281 acatggcgaa gcaaagtaaa gaggaactat ttgaaaaaga aactaaatat tgcaagataa
    14341 taagaagtgt aacagtagaa gatggagatg aaagatttgc aatagagaaa atatatatta
    14401 agtcattaca aagatacgag gttagaatat gcctgtatcg tgactgccga gatagaatta
    14461 ataaacttat tccaaggcct gtagatttaa ctatggataa gtttgtagca ttaatactgg
    14521 taggattaaa ggctggtgta ctagatgatg atttcaagca agaactaatt aaaggtttag
    14581 cttaggaggt agaatatgac aatagaagag tttactagtg gagtaatgtt tgggatttca
    14641 atttttagtg cagtattaaa gcaagtatta gaaaataaaa aatagtatac aggaggtatg
    14701 agtatgattg gatttataat aggaagtata ataggaattg gactatgtat tagtatagag
    14761 atactgatat cgaaaaatgc cataatatac taagttatat ttaaattatt aatagattca
    14821 tatataattt atttaagccc ttaaagccca gataagttat attaaagtaa agtttgtatt
    14881 tatgtaaatt aaataatata tattattata aatttgaaag gactttataa atatgaaagg
    14941 caatataaac aaaaaagata tagatttaag taaaataaca tttgataagt ttttagaaga
    15001 tgaaggatta caaaatagag agagagatta cttatatgta gatgattatg acaatacttt
    15061 agatactcaa gaatttaaat tacatataat tgaagagaat actgataaca catatataga
    15121 taaagataaa tactggtatt tagatttaga tgatagttta tttgaatacg aagaagatga
    15181 agatactatt gctaaaacat tatatagtct aatagatatt agaattaaat ttttgaaaca
    15241 tgttgagtat agaaaatata taaatctagg agtaatagac gaagaatatt ttgatgagat
    15301 taaatcattt ttgaaatttt atatagtagc aaaaacaaag aaaaatgagc taggtaattc
    15361 aaatttaaag cagatggcta tagatatttt tgataaagaa aggtttgaag acgacatatc
    15421 agatgatgat atggaaaata taagtgaatg gataggagaa gatcaaaact ttaaaatgtt
    15481 ttatgataaa gtttgtaggg atctctcaaa ttctacacag gataaagaaa tattaaaatt
    15541 agcaggaatc aggaataata aagtaaaaca ttacaaagga agaaaaatta gtagaaaatg
    15601 taaattagaa cttaaatcat tgactaaaat gtgggagtat gaaactaaac tgactcatga
    15661 tgcaaataag gcacataaga aaataaaatc taatatgctt gaaaaaatga cagataagaa
    15721 ttataaaaat atatatgaat atttaaataa tgagattata aatagtaatg aagaatataa
    15781 aaatatttat ttttatagct tagaattaaa gttagcgaat gaattaagaa agtgtactct
    15841 taaaaactta aataaaatta aagacataga aaatcaagta aaatttattt cttatactat
    15901 tgcttatata cacatagttc cagttttaac gataagagag aaactaagcg ataagttatt
    15961 taatatattg tcaactaatg aaggtaagga tatagaaaaa tttactaaag aagttagtct
    16021 tataatgaga attataaata tagaatgttt ggagttatta aatatagtta gtaagaacaa
    16081 ctattataaa aattttagtg aaaaggaatt agaggagttt aaatctattt ataatataaa
    16141 taatggaaga aagtcatttg aaaataaata taaaataaaa gtagattata atatagatga
    16201 tgtaagaaag tatggagagg ttataaaagc aataaatata gaaagtaaga agaaaattgt
    16261 agatagaatt aagaagaaga gaaaatctaa ttagattttc tctatttttt ttgaaaaaaa
    16321 taaaaaatat ttggtgaaaa cgtttatctt taggtgatat gagtagtcat tatttattat
    16381 ataatcatat ttgtaggaac gagctaatta ataaaatcta agaacaatag cttaatgatg
    16441 aaaaagtacg gcctttcggg ctttgacaac aggaggtata ttaatgacaa actttaaaag
    16501 tgtaaaggta gaattaattc tacttattga atgtgaagaa ggaaatcgta atgaattaga
    16561 aggtatgatt gatgaaggtg tattaaatga aaaagaatat agtttaacta tattaggtag
    16621 tacagagtat gaagataacg caagggctat ttacatactt atgaatacag gaggatcata
    16681 cgaaaaaaac ttacaacgat taagtagatt acacttgaaa attgaaaact tacttaaaga
    16741 tacaaatgtt aaatatagag gtatatcttt agtaccgaat attgttaaat gggataaata
    16801 ggaggaatac aaatgagcaa ggcaataaac agagaagaat taattaaatt atatgaggaa
    16861 ataggattaa atgttttaga taataacacc gttatgaatt catctaataa aaagatatgt
    16921 aattttttag taaatataat agaacaaagg gttatagatg gaaaaatgta tttatgtata
    16981 aatgctatgg ggattaatgg agagctatta tcagaggtaa tattagattc gaataccata
    17041 aatagaacta catgggtgat tgaaaattgg ggttttaaat attctataga taaaaaatat
    17101 attaatgact ttattgaatt gatacaacac ttatgtatga atataaaaac taagtatata
    17161 tatgaccata caggatggat atatcaagac aataagtgga tatatctaca tggaggagga
    17221 gttataggag gttcagatgt tcaagttgag ttagatgaat caatagataa ctatatacta
    17281 ccagataaaa ttagtgacat aaataaagca tgtgaaatga gtttaaagct agtagactta
    17341 atagaagatg ataaaggtat tatattctct tcattagttt acctatctcc acttattgaa
    17401 attatcagta gaaaaattaa accaccggaa aatatagtat ggacatatgg aaaaacagga
    17461 tcaagaaaaa cagcagtatc aaaagttgta ttaagtcatt ttggtgattt taataaaaaa
    17521 attccagcta catttaatga tacgattaca gctattgaat taaaagctaa taagttaaag
    17581 gatagcttat acttatgtga tgactttgca cctaaacaag actttaggga taaaaaaatt
    17641 caagactcaa aggcagaatc cattataaga atgtatggag atagagtagg gaaaggacgc
    17701 tcaagtaata agttagatat aaaggatgct aagagaccta gaggaatgat attaataaca
    17761 ggtgaagatg tgatatgtgg agaatcatct aatgctagat tattatcaat agaattaaat
    17821 agaagttcag tagatttaaa tatactaact gaattacaga ataatagtca tttgcttgga
    17881 gaagcaatga gaggatatat agaatggtta ttatctgaaa tgggaaatat tgatgaatta
    17941 tctaatatat tattatataa ctttaaatct tacagggatg aaattagaga acaatataaa
    18001 gatgatatac atggaagaac tattgagtct agtgcatgga tattagtagg ttttagttgt
    18061 atgttaaatt atattagaga taaaggtgtt atagataagt atgaagatga tgagtacatg
    18121 gatagagcga ttaaagttgt agaaagatta atagaagagc agataaaatt aatatcgaga
    18181 aactcaccta tagatatatt tttaaataca ttaaaggaat caataaattc aaagtcaata
    18241 agaatagcaa cattaaatga ccagaaccaa gttatagaag ataaattaga atatatatgt
    18301 ggatataagg ataaggaata ttattacttt tacacagata aagtatattc ttttatagaa
    18361 tttgaaaata aaaaaagagg tatacatttg ggaataagtc aaagacaact agtgaaacag
    18421 ttaagagata gtggaattgt gaaagttgat tctaacaatg attctcctaa aaaaactatt
    18481 catacaaaag atgctagcga ttcaacagga tatacaacaa taagaccaag aatgcttcag
    18541 atagaaagaa gtataattga gaactttcaa atataactta taatcaagag tgcttatgta
    18601 ctcttgatta tattctatga taaatggagg atataaatat gacagaaact tataaaatca
    18661 gcataggaag aaaagattta tattctaaag actataaggt agttgaaaga actgactatg
    18721 aaaagagaca cacagttaat ataagtagaa gcttagtaga catactttct atacttgtaa
    18781 aagaagatag atagattaaa atatggttac acacttagtg ttagtgtgta gcctttaata
    18841 tagattaaaa ttagtatatt taaactttgg aggaaataat atgacaggaa agaaagcagt
    18901 agtatatgca cgctttagtt cagataatca aagggacgaa tcaatagatg ctcaattaag
    18961 agcaataaat gaatatgcag ataagaataa tataaaaata gttaatcaat ttatagacag
    19021 agcaaaatca gcaacatcag ataaaaggcc agagtttcaa aatatgatta aattctgtga
    19081 agcagataca acaggaatat ctatggtaat agttcataag ctagatagat tttctagaga
    19141 taagtatgat tcagctatgt acaaacagaa attaaaagta aaaggaataa gagttgtaag
    19201 tgtattagag aacttagata attcgcctga atccctaata ttagaatcag ttatagaggg
    19261 aatggcacaa tattacagtg ctaatttagc gagggaagtt gctaaaggtc agaaagaaaa
    19321 tgcccttaaa gcccttcata atggaggcga tgcaccatta ggttatgatg tagcacatga
    19381 taaaacatat acagttaatg aagaagaagc tcaagcagta aagattatat ttgatagata
    19441 tattaaagga tattcatata gtaagataat agatgaacta aatatcttag gatataagac
    19501 taaaagaggt aataaatttg gtaagaatag cttacatgga atattaaata atgagaaata
    19561 tacaggagta tatgtattta ataagactca gagaaaaggt gtagatggaa aaagaaatgg
    19621 acataagcaa aagagtgaag aggaaataat aaaaatagaa ggtggtatgc cacaaataat
    19681 agacaaagga gtatttaaac aagcacaaga tatgattcaa aaaagaaaga aaactccagg
    19741 tgcacataaa gctactacta catatctatt aacaggacta attaaatgtg gtgaatgtgg
    19801 acatgcattt caggggaata agagaaaaga ttcttatgga aatgaatatg tttcatatag
    19861 atgtggatgt agaaagcaaa agagagaatg taataataga gagataaaaa gagactattt
    19921 agaagagttt atattacaag aacttgaaaa gaacatatta aatgatgaag ctataccgat
    19981 tttaagtaaa gcattgaatg agaagctgaa tgataagaat gaaagtaatg cagagctact
    20041 taaaaatcta gagcagaaac tagataaggt aaataaagag ataagtaata tactaaatgc
    20101 tataatgaat ggtatagtaa atagcatgtt aaaagataag cttgatgagc tagaacaagt
    20161 aaagttcaac ttagatttaa aaatgaatga actaaagata gaaaataaag ttgtagatgg
    20221 tgtaggtgta acagaagatc aaataagagg tatgttttca agatttaagg aatttgtact
    20281 agagagaaat atacctgaat gtaagaagtt tataggagac tatataaagg aagtaatcgt
    20341 atataaagac cacattgaag tcatattcaa tgtggtcttt tgttttgttg acaatgatat
    20401 aactcatgat ttacatatta tcacagaaag gggtaaaatt acatagtaaa tatatatttt
    20461 ataaaagtaa atatcagata ttatttagat aaaaattatt tttgaaaata ttagttttaa
    20521 aaaatatatt tatataatta taaaataaaa tggtataata aaactttaaa tatgataaag
    20581 aggtgagact attggcagat atatttacag tactaaatta tcaaggtagt aaaaagagat
    20641 tattagattt tatatatgaa aatacacaag aatatattga ttctgataaa gcttttttag
    20701 atatatgctc aggaacgagt gcagtagctt ataatatgaa aagacacttt acggtttata
    20761 caaatgatag tgaaaattat gcatatcaga tatctaattc aattgttaag aataatgaaa
    20821 gatataactt tagtcaatca gatgaaatga tcataagaga aaaatataat attaatttac
    20881 aaagattaaa taatattttt aacgatatgg taaatcagga agatatgtta ttagaacaag
    20941 aagatgcaga cggtttaata gagctatatt caaattgccc atgtatatgg aaaaataatg
    21001 ttgatattga aatagaaggc aatacaatac agagtatata tgatttaaga aaatgtaaac
    21061 gagacatacc gaatgtgtta ttcacattgt attattcaag tagttacttc ggcataaagc
    21121 agtctatgga gatagattct attagatatg ctattgaatt tatagaaagt gaaataacaa
    21181 agagcattct ttttacaagt ttatttttcg caatgaaaga gtgtgttttt gctaaagatg
    21241 gtcacatggc acagccatta aatatggaaa caggcaagaa aagaatgtta agattaaaag
    21301 caagaagtat atatgacata tttatagata aattaaagga atttaatact gaggagtttg
    21361 tattatcaca aaaggagaat aagtgcttca ataaagactt tagaacatta ttagaaacag
    21421 aagaaagtat aaaagatgaa gttggtttta tatatataga ccctccgtat acagatatgc
    21481 aatactcaag atattatcat attcttaata cagttgtttt gtatgattac ccagaaatat
    21541 cattgagtag aggaactata tcaaaaggga tatatagaga aggtagatat caatcacctc
    21601 ttagtcaaag aaaacatgca ttaaatgaga ttgatttctt atttagtttc tgtaaggaaa
    21661 ataaaataaa tgtagcatta agttatgcat atccaataga tacagaaaaa caagcaacta
    21721 atagatatac ggctagtatt gatgatttga taaatgtagc caaaaaacat tttggagata
    21781 atgttaaggt agtacatgaa gaatatagtc atgcaaataa tagaaatagt gataaaaaga
    21841 atgtacttga atatttaata atgggtatac acaataagga gtaaatatgg ataaaattaa
    21901 taaattaaaa gagaggatag ctaatataga agcgacgaat aaaaatgata tatattcttc
    21961 taacttatat tggtcacaaa aaccattcaa tatatgtgat gaaattattg aacatttaag
    22021 tgaagatgga gatataatat ttgatccgtt tatgggatct ggggttacag ttatagaggc
    22081 tttgaaaaaa gagtataaaa gaaatgctat tgggtgtgat ataaatgatg caccaatatt
    22141 tatagtaaaa acattattag aaaaatataa tgtgcctaaa atagagaaag atattaaaga
    22201 gctaaataat aaattaagta aattatcaac ttactattca acaaaatgtc gtgaatgtgg
    22261 agaagtagtt actatatcga aaactatatt cgataaggag aataggaatg caaagtgtga
    22321 gctaaagtct atacattata aatgtaagga atgtaaaaat gactttaaag gtgaaccaaa
    22381 tgaaactgaa atatctaaat tctttattga tgaagaatta aagaatatag atgattttca
    22441 attcatagcc aattcaaggc ttgcagttgg cgaaggtgat acagtaagaa ctattttttc
    22501 aaatagaaat ttaatagtat tagataaaat attaggacat atagatgaat ataaaaatag
    22561 tgaatcttat gagcctatga agtatatttt attatctatt ttacatttaa gtaagattac
    22621 agatacacat tctaattctc agtggccttt atggattcct aaaaaagact gtgttgaaaa
    22681 aaatgtagta gaattattca taaagaatag taagaaaacc attaaggcat taaaactagc
    22741 atctaaatat ttatgtgaaa atagagaaaa tgtagaacta atcaaaaatc tagatagagg
    22801 gaaatttaaa ttgataaaag atggagttca aaatatagaa gatgatgtta tacctaatga
    22861 atttgtggac ttgataataa cagatccacc atatatggga caagttttat attcagagta
    22921 tatgcagtta tataaacctt ttatggattt ggaattcgaa ttagaaaatg aaatagttat
    22981 atcaagtgcc ccagctagag ataaagggaa agtattatat tttgatatga tgaattctgc
    23041 atttgaaaat atatctagag tattaaaggt aggtaaatat atgtgtatgt acttccatga
    23101 tagtaatcta gatgtatggg ataatataat agatataatg accaataata atttgaaata
    23161 catgggacaa attcatgtaa gtaagaataa aaacactctt aaaaacatac ttagtcctaa
    23221 gaagtcgtta aatggagatt gtataatttt ctttaagaaa gatttaaata taaactatga
    23281 tataaatgaa gtagataata tagaagataa gataaataat attgcacaag ctattataga
    23341 tataaaagga tacgcatcaa ctccagagtt atatgataat ggagcactag agtatataat
    23401 atcaaacgga aaactaaaag agatttctaa aaagtataag gatttaacaa gtatatttga
    23461 gcaaagattt aattgggata aggaaagagg ggtatggact gtataataaa taaaaagcat
    23521 ctatagataa ttatagatgc tttttattta tattttatag taaactaaat atttaataat
    23581 ttctaatttt tatacaatat gttaaaggat tggctatcca aagatattat ttaatataat
    23641 tttttagcat taactctagt taaatcactt ttagataatg aaacctgtaa tcttctgata
    23701 ggagaaccct tttctaaaca tctattgatc ttttcctgta acttcttacg aagaagagga
    23761 actacatctg ctggttcagt tgagtagata tgttcaactt ctatattttc tttatcaaca
    23821 actgctaata ttatagcttt atcattataa tatttattta gtacattatc agatatatct
    23881 tgaaagttcc aagcatgatt tttagaaact ttatattcat aaggattacc ttcactatca
    23941 taggcatcat gagcaccttc tcgtccacct tgctctgggt ttacttgatg gtttaattct
    24001 aatgcaacta ttaattccca aaacttatta cttgtaagta aatttttaac tcctgttaat
    24061 tcttcaagtt cacttgaagc tttataaata gcacttagat atttatcata aacaccattt
    24121 tgagctgatg ggattatctt acttgcattt aatctagact ctagttgatt agggctgaca
    24181 tttatcattt ttctggaatt ttgtatactt tgagtatact ttagtaaata ttcacacatt
    24241 atatctccag gtatagagta ataccaatca agctttccag ggtaaggaaa atcaacatca
    24301 tttatatgag caaatataac tgccttagct ttctttaatt ttgctatact tgtatccgaa
    24361 aaatcattaa acgtccaaga atggttagag ctacttttct tgaaatgttt atattcaaac
    24421 tcatttccga attcatcttt tgcatctcta gaaccactat gtccaggtat tagtatgtga
    24481 ttaagtgaat tagcagtcat aacttcatat attttgctat tagaaagtat atcatttact
    24541 ttatattctt taaaaatacc atcttgtata gcctttagtt catttatttt ctgtgttatt
    24601 aaaacatttg ttatattatc tcttatcata ttatcatagc tccttaataa attagtattt
    24661 ttagataagt catttttaat tagatttatc acataactat tgatgctatc atttttaaat
    24721 gctactaaat ccaataaatc tttatgaaaa tcatccttga gtcttatagt tagttttttc
    24781 aagctatcac ctcggatatt aattagatgt gccgtcgaga taagtataat taagcaggtt
    24841 tataaagtca aggattaaaa aaattaaatt atagatatat aaaagtatat atagcaatat
    24901 agataaatag ctaaactatt aatcgtgggt attattcttg cgattcgtct ccaccaattt
    24961 tgaaaaaaga atgatgaaat acttggaaat actagtcttt gttgactagt attttttatt
    25021 ttggagaaga aatccaccaa ccgtacccca accacagaaa atgaggtttt tttgggggta
    25081 tattggggtg catttaggct gtataaaaag tttttatttg tttattattt tatttgttta
    25141 ttattttatt tgtttattat gataataggt aacatagaat tttaatatct actttatgat
    25201 ataatatttt tataaatgta agacagttta agttgttaat tagtaaaaat atatattata
    25261 taagcttgat aggagttgag gtttgtaatg gatgaaagaa aactagaatt ttctattttt
    25321 tgcattgaag ctttagcaga aaaattgaat atagattcag aaggagttta taaacttctt
    25381 aaacatgata ctgatatttt agataactat ataatacctt gctatgaagc actacattct
    25441 caaagtaaag gttacatagt ggaagattta atgcaagtac ttaaagaaaa gggtgtgata
    25501 taatgattgt atatcatggt tcatattgtg tggtaaagac gcctagtatt accttttcaa
    25561 gagattcttt agattttgga aaaggattct atgtaaccag tataaagaca caagcaatta
    25621 actggacaga gcgttttaaa aaaagaggta aaaaagcata tttaaatagt tattctttag
    25681 atattgataa attaaaagaa aactgcaagg taaaaatctt cgatagttat aatttagaat
    25741 ggcttgattt tataatagaa tgtagaaata tgagtgatat atatatgaaa tatgacatta
    25801 taataggtgg tattgctgat gataagattt acaatacaat agaactgtat caagataatc
    25861 ttattgaaaa agatgaagct ttaaaaagat taaaatacta taaaccaaat gaacaaatat
    25921 gtataaccaa tcaaaatgta atagataaat atttaaagtt taggtatgaa gaggaggtat
    25981 aatgattatg gaagcaaata aaacattatt acaaatacta tataaaaaaa tcatattaga
    26041 attttccaaa caaacaggta agagcttgga agaaagtatg gattatttat atacttctga
    26101 aacatataaa cttataagtg agggtgtatc agatatgcac tgtaagggtc atatatactt
    26161 ggctcaagaa ttaatgttag aaaatggatt aatgtaccat aagggctatc caagataaat
    26221 aattaaaact agatacgaaa taaggataca atataatata aaattaattt gtatccttat
    26281 tttgattttg taattaacta tatgaaatca caatatttat atgtatttga tttttacaat
    26341 gaaaatattt tatccaactt ttctacagct tcaactttct tatcttccat aacttgagta
    26401 tatatattca aagttattga tatatcataa tgacccatta aaacttgtac agttttagga
    26461 ggaacatcat tctcaaaaag tctagtagca taagttttcc taagaccatg aaactttata
    26521 ggctctatat ttaagctatt taatatagac tttaaattcc ttaaaggtct tttatcatct
    26581 atagggatgc ctaattcatt gcaaaataca taattattat ttatatataa ttctccagcc
    26641 tttaatatat ctgtattttg atttacttta tgctctttca atttatttaa aacatctttt
    26701 ggaataggta cagttctgta agaatttgat gtctttggcc cttgctctaa tactttaagc
    26761 tctctatttc cagttttatc tataaaatat gttctttgaa gagttctttt aactgttaaa
    26821 ttagactttt taaaatcaat atcaaaccat ttaagtccta ataattctcc taatctaagt
    26881 ccagtaccaa gagcaagtaa aaataatacc tctaatttat gcccatctat agcttttata
    26941 aaactctttt gttgttcaag agtcaatatt tcaagtttgt tttctttttt ctcaactggt
    27001 atatttacca tagtacaata gttacgttgt ataagacctt gtttttcagc ttctgataag
    27061 caggttttca gtttagtgtt tatttgcctt atagtaggta tacttttatt ggaatcaagc
    27121 aatctattgt aatatttttg tatgtgagat actcttaagt actttaattt aacattgcct
    27181 aaatcagtat ctttaatata attacgatag atgctttcat atctttcaaa tgacttgggt
    27241 tttaaatctt ttattctata ttcaaataac caattgtaaa accaatgctc taaagttatt
    27301 ttatcatctg aaatatcaga gcttaaataa tattgttttt tataagcatc taatttatct
    27361 ttaacttcct tttgagtctt accataaaat tcttttctca gtggtttacc atcttcatta
    27421 tagccaatca ttatacggga acgccaacca tctttatatt ttccgataga gccttctcca
    27481 ttagctcttt tagttgccat ttttaaacag ctccttatat gatttatagt tatatccatc
    27541 aaagaatgta atacatttct ctaataagaa ttttataact tcatcagctt ctagtgtttc
    27601 atagaagttt agcattaatg tgttgtactc ttgtttatgt ttagctggta ttgatagaac
    27661 tcctatatca ttatttataa gtattaggtt tgaggtaagt ctagcagttc ttttgttgcc
    27721 atcatagaag aattggcaat aggacaacca taaatttatt attatagcct tttctaatgg
    27781 gttagttatt ttatttatga tatttatgtc atttacaaat aaatcattta atagagtggc
    27841 ttctatgcat tcatattgag tagtaccagc tatacctata ttaccatttc tgaaatctcc
    27901 aacgattaga gcttcatctt ttgcaactat tttatgtata gagcaaattg tatctttatt
    27961 taaagataag ttttctttgt tacttaattt tattacataa tcccatgatg attttatatt
    28021 tagtatttga ttttggtcac ttaatttatg tccgcctacg gttataccat caagtaatgt
    28081 ttgtacttct gggaatgtga aggggttatt ttctaggtta cccatatcat atacaaattc
    28141 acttatcatt cttctaatgt atgttaaagt atatttttta tctccatgag gtatattagt
    28201 agggaattta tgtgtgtatt ttggtatata tgatttattc atgattttag ctcctttcat
    28261 atctaatttt tatttattaa ctatgaatgt ataaaagtta ataggtaaac tcatattttg
    28321 aaaatgtgca atactaatat tcttcatcct catcttcaat aagaccatta gagttttctt
    28381 gtataaaatt ttcaacaaga ccatttatca cccaatttag atttttggag aagtctttga
    28441 agtcagagaa gtttaggtta attggtttta ttgtatctgc attatctgta atttcaaact
    28501 tttttttata gtctgttttt atatcataac cataattttc taatatgtga attactaaat
    28561 ccaactcttt atcaaatact tttaaattaa tattattatt tgttaaagta tctaaatcat
    28621 tttcttcaac aatgagttta tttatagcaa ttgataaagc agtagctatt tttcttatca
    28681 tatctaaact aggcattctt tgattttgct catatttagc tatagcatga actgaacaac
    28741 caatcttgtt ggctagctca tcccttgaca tatttatcga ttttctatat tgtttaatct
    28801 tttctccaat tgtattattc atcataattt caccttaaat atatcatcaa acaatgattg
    28861 tacttctaaa catgtaaagg agttattttc tagattatcc catatcatat actaaattac
    28921 tcataactct cttagtgtat gttagagtat attttttatc tccatgaaat aaattagtag
    28981 gggatttata tgtgtatttt agcatatatg atttattcat aattgtaact ccattcatag
    29041 ttttttatca ttgctttaga tagtattgat attattttat attttagtta gtagtttttt
    29101 tatttcttta agtgtatttt caagcttttc atttttttcc tttagttcgt tattttcttg
    29161 taataattta aagtaatcat tatttttaat agtaatattt tctttttgag atattaattt
    29221 ttttagtcga atattacttc cattatctgt tgagtcacta aagtaatcat agattgtttc
    29281 tatactttct tctgatagac caatatctaa taattcatca attgattgtt ttgtaagaac
    29341 atcattttta ataatttctt ttatgtcatc aggatgtaat gaaaaatcta attcagtatt
    29401 agatatacaa tctgaaagta tccaattata aacttgattt tcatttagtt ctaatttttc
    29461 accaagttta taatattggt ctttatcaga ccaggcacct agcaataaac ttgaggtaat
    29521 tctaatatca tcttccataa gatttgaaat agcatttaaa aattcatcaa aagaatagta
    29581 ttcttctggg aataaagact gtaaaaggta taaaacatct tcattagtca ttacttcatt
    29641 ttcaatatca aaataaaaga tagtcttttc aattttcaaa gctttagcga gttttgataa
    29701 ggtatctata ctaggtattc ttttatcatt ttcataattt attagagcat ttcttgagat
    29761 gccactaatt tcagcaagtt gaatctgcgt tagctctttt tgttttctta tcttttttaa
    29821 gttttttcca atttgcaata ttatcacctc gtataaagta tatcccaaaa gtacacaaat
    29881 gagaacagaa aaataatata ttcttattaa cagtacacat atatcgacat tataattaaa
    29941 atgtaaagtt tacgaatgta aactagaaaa aaggaggaaa aagtatggga atcaatttaa
    30001 aaatatttag gattaaaaaa ggtctaaagc aacaagaact ggcagataga gttggtatat
    30061 caagatatta tttatcaaac ttggaaacag gtaaggctaa taatccaagc aatgatttga
    30121 tgattaaact atctagagct ttagatacaa cagtagaaga attatttttt agtaaggagg
    30181 attaaaatat gaaagagaat aataatttag aggttttaaa gacattacaa gccactttaa
    30241 acattattgt aaagaatgaa gaagaagcac aagaaaaaaa gcaaggatat aaaattattg
    30301 aaaatgcgaa aagaaataaa gctgagctta taacatgtaa agaagcatct aagctttatc
    30361 ctataggaga atcaaaattt aggcagttat gtcattctaa aaacaaagga ttcccttgta
    30421 tatggataaa taatcgagta tacataataa aaaataaatt agatgattgg tttattgaaa
    30481 atgcaaatgg tatcaagtta tagtcttaaa gtaggtgttt gcaattgagc aataaagggt
    30541 ggataaagct ttatagggag ttgttggata aatcaatttg gaaagatatt aaaccagaaa
    30601 gaaaggtaat attaataact ctattactta tggctagtca taaagaaaat catgttttta
    30661 tagataataa aaagataaaa ataaagtttg gacaatttat ttcttcatat agcaaaatag
    30721 ctgaaagatg tggtaaagga gttagttatc aaatggttag aagttgtttg ttggttttta
    30781 aaaatgctgg ttttctaaca tacgaaacaa caaacaagta tatagtaata actattgaaa
    30841 actgggagct ttatcaagag aatatagaaa atcaacaacc taatcaacag actattaaca
    30901 atcagtcaac aacaatcaag aatataaaga atgaaaataa tattatatat agtgaaatca
    30961 taagcaaatt taactcaact tgtattgaac tttcaaaggt caagaaacta acagatacaa
    31021 gaaagaggag aataaattca agaataaaag aatatgataa agaaactgta ttagaggtta
    31081 taaatatagt tagtaaaaca agttttctga atggaaataa taatacagga tggaaagcaa
    31141 attttgattg gataatgaat tcaagtaatt ttactaagat actagaggga aattataata
    31201 gcaaggaaat tgtcataaat aaagagacag gaatatataa tccagatgac tcacaattaa
    31261 agtggtaagg aggttatatt ataaataatt caaacaactt aattaaaaat acagaagcag
    31321 agcaatcaat taaattacac taagttagtt ataaatcttt catatatttg ttttttaagt
    31381 atctctaata tcgtttaaat tacactaagt tagttgcaga tataccagaa aatatatata
    31441 aaattatgcg taaactatat tgttatgagt acaaataata tttacctatt cattaaaaaa
    31501 cttaactaag aatttaattt atattattga agtatcaata attttggaaa gtagaaaatt
    31561 ataccccaat aataccccaa ctacaaaaat agacaataaa aaataaccag aaaaattgct
    31621 tcaacatcat gaccaaatag ccatatacct atacttataa aaaccaacaa aacaatcaaa
    31681 taaccactta taaattctcc tcggtctcca ccattggaat ccacgaaaac atattgattt
    31741 aatagttaat atgtttttta ttttgaaaaa tcatatataa tataaattaa tagtttattt
    31801 aaaaatttca gtatgagcac aaaattatat aattattggg aattagttat aataagtaat
    31861 ttctagtaag aatgatttgc aaaaaataat aatttaaata caaaaaacta aaggatagaa
    31921 gtcataaaat taacttttaa cctttagtta tataaataaa aaattagtta ttactaaatt
    31981 aaaattagat ttttaaagaa cccaatatct ctattatcca tatcaccaat taacatgctt
    32041 ctatcaagta ataaattata ctcctcacag ctagtttcag gcaataaagt acatgaatgg
    32101 catgctccta gatttaaaga atttctacct tgaccagtac tatcaataca tattgggtca
    32161 ttagaacacc aaagagcacg attaagagca ctatttaata tagatggtaa tgaatcatgc
    32221 ataccttgcc taactaatcc tcctaaagta ccttcagagt caccacttgc agtgtaaact
    32281 aaaattccag acataacttt ttcattttcg gaattattac aatatatacg ttccctaagt
    32341 gaagaggtag catatccaca aacaaaacta agttctctta taattaaatg agctaaagta
    32401 tgtagtaata agaatttagg tgtaattttt ctataagctt tgtcattaac attaatatct
    32461 ttattatatc tagaatttaa aatagatgca ttatcctgta ttttaggatt agatgaaatc
    32521 cagcttttta ccatatcatt atttatctct atgaaaatac cttcacctct aacctcagat
    32581 gcaggataaa aagatttttt acaatctcta gatcttaaac ttataacctt agtatcatta
    32641 cagctatcat ctatatcttc ttcaccaaat atattattat caggtggatt gactctacta
    32701 aacccagtta atgcccttac ttctctaagt ctttttacta acactatttt actgaagttt
    32761 ggaatatcat aatcagatag attttgttct tctattgtaa agtcattagt ttttttagat
    32821 ttagcatcta tatttccaat taaagcctca tattcttctt ctctatattg atttctagtt
    32881 gttgttgatg gtgaagaatc ttcaaaaggc aataaatctt tcaaaactat ggatacttta
    32941 tcagtatcaa aacctgtttc tttagcaata tcactggcaa agtcctcaaa atcctcatcc
    33001 ataaagcgtt ctaacttatt ctttttaata gcacgatcta agaatttctt aaatgtatca
    33061 taacctttac tagatagcac tttagaatta agagtatcag agtatggtgg tataataatt
    33121 gaactttcta tcttaggata atatacatta gaggcacctc tttgagaagc aaatgaatat
    33181 ttagtgcatt tatttacttt tttattatac caaggcgaat taccagagca tttaaacatt
    33241 tccttatatt cactaggtac accataatca ttagatttag aagattttaa acgcataaat
    33301 gcgcccttca tattagcttt agctccacag ctacattcaa gttctatacc ttctaatcct
    33361 aggttacttt ttccagtctt cattaatatt tgaggatttt cacaaatttc ttttcttcca
    33421 gatttatttc taaaatgaac ccattttacc catggaaaat catcaatatg accatgttca
    33481 caaatagtaa ttataccagt aggtactaat tgtttagtag acttttttgt gctacaagta
    33541 aggcaaatag gtattttcat aggtttatct ttatgagcat ttttataaag tttctcccaa
    33601 tcttctatag gcataaattt tctacatttt ggacaaaagt accatctagg aaacctaaca
    33661 aaaggaacag aagatctatc atctatagtt ggaggtgttc ttagttcatt aaccctcaat
    33721 gtagcctgaa gcctatcatc ttgtatttta attgtattag tccaataatt tggatgagca
    33781 gtcattaatg tttgatcaat aaaatctagc atagctcctg gtccaaaaac acttgtagct
    33841 tgatttattc ttaaattatg agttttatca tctaatttta tagacatatt aattttcctc
    33901 caattctata atttcaacag tacattgttt atcaacattt ctcatagaat ttaatgtttg
    33961 tttttcattt gtaataacat ccttatcata aggaattaaa agattattat atattttatt
    34021 atcaaattta ctagggcgct tataataatg caataaatta tctttatcag atatttgagc
    34081 tttatcatgc cataaatcca ttataatatc taattcagat gaaatctcag atttaaattc
    34141 atttactata ttatcatcag cattttgatt attttctcta aatatatcga tagatctatc
    34201 tattatgaag ttttttaaat cttctacacc atccatattt tttgaaaatc gacttgcaag
    34261 atcattagct tgaagtccta aaatatgtct aatcatactt attaatactg catgcatgcc
    34321 tctttctcta gctggttcag agaaaggtgt aatactagta ggttcaacaa atttatagaa
    34381 tgattgatga taactaaaga actgttcata atgagatcta tctcttgttt ttgtaggatt
    34441 atataatgta aatataagtc cttcattact tctaccaact ctactgctag cttgtatgta
    34501 ttcagatgtt aacttaggct gaccaactac taccattaaa tttaatctat caacatcaac
    34561 accaactgaa atcatattac tagctaatag agtatcgaca gttaaattat tagaagtaag
    34621 agtattagat agttcatcta aagttgtatt tatcttagta gaagtaagtc tactagtaag
    34681 ctctctttgt tcatttaggt tccttaatga ttgataaggg tgggctgaga ctcttttaca
    34741 taatctttcc atgttgtctt ttatatcgtc atttaaaagt gtgctacact tacctaattc
    34801 tctgatacta ttaaaataag atactagtgt ccaatattta tccaataaat tcaactctac
    34861 attctccatc attttaagtc tttggagtaa tgctgacata agtctaacct ctacagtaac
    34921 ttgagttttt ccagatggaa taacacctac atatagtcgg ccacgttttt tacttaaagg
    34981 catctcttta acaaagaaag agttactaga tgttaatcct ggaggtggaa actgagctac
    35041 attacgatta tatagatttt taacttgatt ttgtgctttt cttatagtag cagtagaagc
    35101 tataatctta ggttttatac ccttatagct acacatagca tcaagtgcag tttcatataa
    35161 acctaccata gtacctaaag gtccagaaat aagatgaagc tcatcttgaa ttattaactc
    35221 aggagataag ttgctattat catcatctaa agcaaaaaga ctagctactt ctgtagtcca
    35281 agttatcatt gcaaatttat caactgtacc aaataaaagt gttggagggt tattataaat
    35341 ctcctcatct acaacttgta taggtaacat atcttcaaat gaacaatctt cttcaggaca
    35401 gcataagtag aatttcttag gtctagattt aattctatag ccccaatcac caacagcttt
    35461 tttagaagtt ttatcccatt ctttaacaag ttttgttcca caccaaggac attttagtaa
    35521 ttggaattta ttatattttg attttaaatt atctaactca tattcactat ttatactaga
    35581 tctaaactta atgttattac ctgttaagtt attaagcatt tcttctgcct catcattaaa
    35641 gtttggagtt tgatcactcc caatccacaa accaatagaa atctcttttt tcccaagttc
    35701 agacttattt tcttttctaa taatttcaca ggcacaaatt aatatacttg ctcttatgaa
    35761 ttgttgtgca gccaatagcc ttagtgtata tctcattatg atagctgtac cgttaccatt
    35821 ttctttgttt cttattcttc ttaagaagat agtaaaagca gatagaccta agtaggcctc
    35881 cgttttacct ccaccagtag ggatccaaag aaggtcaact aaatctctat gtttagagtc
    35941 aggattttct atcgaagata aacatgtaag tatataagca agctggaatg gtctccaagc
    36001 agaattatca tttgagtaat ctatatagtc aaggttatta gatccaggga gtctatccgt
    36061 aatatgtata gaatgacatc tttgcataag tatagctcta ttcattagag aaaatgctct
    36121 gtatacttca tcattagact taattaaatt tataccttct agcattctag acttagtatc
    36181 tctgcagttt tttatatgct catatgctac atctttaaaa acatcactaa gatcttttga
    36241 atcttcttct agatcatcta tccaaatatc ataagcatca acaaacttat aaagaaaata
    36301 tattttctta tctttactaa gatcagagat atctgataaa tttttcatag acagtaattc
    36361 tctagaaaca tcacttaatt catctacatt aaagtccaat ttaggtactt caaaatgagg
    36421 aataaactca gtacaaagtt ttattaaagc atcattttga atatcttgat ctatagctac
    36481 accatgacca gtagcaaaag tttttttatt tcggtaaagt aagtctaaag atatttcctc
    36541 agaatctaac tttttaaaag tatttactga tccagctgta aaaatatcta taaactctaa
    36601 atcattgtta tcttcagcta ataccattag ttcaggctgg aaaatagtat tattatattt
    36661 cttaggccca gattgactat taaccagcat acaagtaata ctaacaaagt catttgaata
    36721 aattctttta gataaaacta tatctaaatc tttagatata ggttttttaa tatagttttt
    36781 atccatatct attgatacta ttaagttttc atgtggaaca cgaacataac caccagtctt
    36841 attctcatca tcagggcatt ggttagctaa cttgtataaa acaaatttag cttcagcatt
    36901 atcaagtaaa tcatgctttt ttagtaaatt ataaaccttc tcagatgtta gtttttgctt
    36961 aagatatagg aatgatcccc taagttctac atattcatta attgtattgc tagaatagaa
    37021 gtcgtcacca gtatatttaa catgacagtc tgtatataaa gcatttctat aagtagcata
    37081 atttaccctg aaatttatta cttctgcatc tttttttagg aaaaaagtca tacccataga
    37141 agaaggtaaa gtttcattag acatacttat agctctatct agttcatttt gtgtatcatc
    37201 tgatgtaaat acattatctt caccatcata ttcagatgta ttacttacat caatattgtt
    37261 attaagtgga tataaaatac ctgttgaata acgctctatt ggactatctg ttattatttc
    37321 atattttata ttttcacaaa gatcttccga accaggtcca agaagttctt ttcttacaag
    37381 atctagggtt ttatctctta gttcactaca attcatattt acctcctatt tatttaataa
    37441 acactatata agtctaaaac tttgttaaca aggtggggtg acagatactt atcacaactg
    37501 tgtatttctc gtatttgtac aggtgtaaca taatcatttg tattcccaag catacttaaa
    37561 acaactgcag acacttcatc tttaattata tctgatttac tgctaaagaa attatcagat
    37621 acaaatgtat ctatagattt ttttatttta gtgtgcaaag agcgtatact tttagtagca
    37681 tcatatacct cggtatgatc taatttagaa tctataactt taaatatctt ttcgaaagtt
    37741 tttaaatata taggacctat taatctttta tttgttaccc agtttttgat tgttatagga
    37801 ttaatctttt cacctaaaac agctaaactg tttgatacat cattataatt atatttacta
    37861 taagatatat agtttttaag ttctgatttc catgaattag ttaatttata atcatcaaga
    37921 taatgagatg gcaataaatt aagttctaat agcttttgta taacattaat aactaattca
    37981 ccctcttcat ttaagtaatc atcaacaaat aaaaaccagt cattaacttt agcctctgaa
    38041 agtttctttt tcctaacact ttttttatcc caatctattg aataggctat tgcctttttt
    38101 gtacagaata tatactcatt attatcagaa atcatgaatg atgtaacatg ttctttttta
    38161 gagttgtaaa tcccttgatt tatagataga gataatataa tttcatttaa atttatatca
    38221 aatttataat tagatatata ttcatccata gaatcatcat cagaaatagg actatcggta
    38281 gaataagctt ctaatttatt ggtttcataa gttggtttaa aaggttttat atcaagtaag
    38341 ttatatattt tattattttt ttcaatttta tttcttgcag aatttatttg attcatatag
    38401 taattatatt tggataattc actattatat aaatacagtg taatatttat aatcaaatca
    38461 ctatttaaaa tatctaaata tttcaaatca tagaatccaa acataataag attttttatt
    38521 gtttttgaat ctttaaattt ttcagcagaa ataacttgta aatttatatt tctagatttt
    38581 aataaatttt taagtataat agcttcatat tgtttttgta gcaatataac aggtgtattt
    38641 ggtgtaaatg ttttgtattt atatgaagat actaatttat ttagtgtatt gaatttggta
    38701 ttattatcag ataattcatt tataattctt tcaaatttga ctataacatc atcaaataat
    38761 gcacttaatt ctaaatctgg gctagtactt gaaattaaat attttatatt ttctagcttc
    38821 gctgaaggag ttgctatatt taaatttaaa agctttatag tttcttctaa aaaatctagg
    38881 ttaaaaggtg tattttctaa gattttaaga agagagtaac tacacatcat aagtgagttt
    38941 tttatggaaa tatcaaaatc atacttagat atgtaataaa gtgattttct agcctgatgt
    39001 atcaagtcag agatattaga atcaacagaa gtagtacata tatttttatt tataagttta
    39061 tctagccata atttctgaaa agtagaggta ttttcaaaat cttggctata ggttaacaga
    39121 acatctttag accaagagta tatatttaga ttatctatat ttggtaaatc taatatattg
    39181 cttatatttg aatttgaggt tacaatattt acactttcta tcctttttct atttaaaatt
    39241 ctttcaaaat catttatatg cttataaatt ggataatcac ctattataaa tacatttttt
    39301 atagacttat cagatttaat aatttcaaat gcactagaaa tattagttgt aaatttaagt
    39361 aaaggtattt gcttagcgtg atttccagga taatcagttt cggcaccagt tgatgaaata
    39421 tatgaacatg gaaatagttc agtaaactta taagtattat taatatcttt tattttaata
    39481 ttactaacta agtcagatat atcatcttta caaggcaata taatcagtgt agaatcattt
    39541 attactccag tcatattttt taagtctgaa aaacctaaag atgatagaac attctttgtt
    39601 tgaaaagtct tagatgctgg agttttgccc atagaattga cttctaaatg accattatag
    39661 gttataagca tataagccga gtcaataggt ataagtgttg tatccgtttt atttttatgt
    39721 cctaaagtta tgtgtgtatt atttatattt ttaaatataa ctatttgacc cttgtagagc
    39781 aggggcatac ctttatcgag ttttgtaaga atgtgattat ttgggtttaa tgtattattt
    39841 aagtatgagt ttatagctat attcaacaaa taaatagcat atatgtattt gtcattataa
    39901 ttgataatta agttattatt tttttcattt ataatataat ttaatacatt aatattatct
    39961 tgataatact tagaatttgt tagcctttgt ttattaaaat aaatttcaca tatatcatta
    40021 tcaagaatat aatggatatt tgtagttaac atttaaatct cctttagtag ttactattgt
//
LOCUS       DAMJVI010000026        38870 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.026, whole genome shotgun sequence.
ACCESSION   DAMJVI010000026 DAMJVI010000000
VERSION     DAMJVI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38870
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.026"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            94..996
                     /locus_tag="PC545_001453"
     CDS             94..996
                     /locus_tag="PC545_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456225.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKTVIALWVVLIASVSFGV
                     YKNFTAIDQHTTHEKEVIELRLQDTNGIENFVKNFAKSYYTWSNSKEAIDARTQAISG
                     YLTKELQDLNMDTIRTDIPTSSAITDVIVWSIEQSGTDTFSATYEVDQQIKEGEQTTA
                     VKATYTVKVHVDAGGNMVIIQNPTLAPAIEKSDYEPKTPEADASVDADTINDATAFLE
                     TFFKLYPTATEKELAYYVSGNVLESIGRDYLYSELVNPIFTVDGDNVKVKVAVKFIDN
                     QTKATQVSQFELVLHKESNWKIIG"
     gene            complement(1195..1530)
                     /locus_tag="PC545_001454"
     CDS             complement(1195..1530)
                     /locus_tag="PC545_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456226.1"
                     /translation="MTHMSADKLLLGFHLDHVSNLNELLRKVAHNNICIMSDRMTKVA
                     VWKPSVKTIQLVCMEYLNYIIDHSVIYEKIQCANWNGTEETNSIFNNSPSPLITLIYS
                     CDLQQNKTT"
     gene            1480..1689
                     /locus_tag="PC545_001455"
     CDS             1480..1689
                     /locus_tag="PC545_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456227.1"
                     /translation="MVKVKTKKKFVSTHMCQGEMPIYVRERYEKMLAVSIRAPNLEGM
                     VENFDKDLSRPNETDLIELKNAISK"
     gene            1782..4445
                     /gene="mgtA"
                     /locus_tag="PC545_001456"
     CDS             1782..4445
                     /gene="mgtA"
                     /locus_tag="PC545_001456"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2456228.1"
                     /translation="MSKATLFDSRIKKYAYCRTSEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFVASNFARNATTAIII
                     FSMIVISGVIRLIQELRAKSAAAQLDRLVHESVTVRRDGKLIEIPGEELVVGDIVLFS
                     AGDRVPADIRLTKITDLFISQAAITGESAIIEKSCRKLSYGEQDTLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKNSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITDGKWMESFAFALSVAVGLMPEMLPMVITACLAKGSLAMSKKQTIIKDINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDIFGNENAEVLDLAYLNSCYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNHVEYRGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGIYTERILTG
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLQLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIAFTSLTFVGGASLFGLTKLPLW
                     YFVFLLLVVSLYMLLNSVAKYFYKKKYQELI"
     gene            4461..4964
                     /locus_tag="PC545_001457"
     CDS             4461..4964
                     /locus_tag="PC545_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2456229.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDCAKDLLSGIQEMIR
                     GEVSSLLLGASEESDWPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYILDNSNIMAFIRKLRKKIEPAPDCPTYILTIWGIG
                     YKFNDQI"
     gene            5102..5779
                     /locus_tag="PC545_001458"
     CDS             5102..5779
                     /locus_tag="PC545_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018597221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2456230.1"
                     /translation="MFYTDFILRIALSLTLGFLIGLERQLTGHPAGIRINVLICMGTS
                     FFTLFPMLYGSDQVFRVGSSIISGVGFLCSGVIFKDRGTVRGMNTAATLWCTAAIGIL
                     ASTGMYAMAITAATILIGSNLILRPFARKFHPITSSDESEKQYRISVTCQEKAEQDIR
                     LLLINSNSCKTLFLNNLESGDVVGDKVEIVAEYCSVGKSKNNILEGIVGQVLVIPEVI
                     SAGWEVL"
     gene            6026..6337
                     /locus_tag="PC545_001459"
     CDS             6026..6337
                     /locus_tag="PC545_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2456231.1"
                     /translation="MDFTCIFFGILFTLAGFWLAFGKGYRHLSLWKNMPQEEKDKINI
                     VPLCRNVGEMIALNGIIFLMKGFLPGFSNHWFVFAIIAWLIVAGFDVWYIEKSVRYRN
                     Q"
     gene            6503..9268
                     /gene="mgtA"
                     /locus_tag="PC545_001460"
     CDS             6503..9268
                     /gene="mgtA"
                     /locus_tag="PC545_001460"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDF2456232.1"
                     /translation="MNKKENRMAIRQTVEKAVIRDEQNRRIQFAATNPIKEVLKKLHT
                     TLRGLEVDEVSVSRAKYGTNKVTHEKKKSLAKRLVGAFINPFTAILFCLALVSTITDM
                     IFPYFSLFGSVPEDFDSLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNYEKKELPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTNIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLAAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEAADSRLIDLSENYVKVDEIPFDFTRRR
                     LTTVVQDNKGKTQMVTKGAVEEMLSICAFAECNGGVQPLTDDVRYRILETVDNLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECDMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVAESTDVFAKLTPDQKARV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIFLNLIYDLSC
                     TAIPWDNVDEEFITKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFVQSRAS
                     APVTLLTMTGIIVLTIIPFTVFGTMLGFVALPAAYFAYLIPCILLYMTLATSLKKAYV
                     RHFGELL"
     gene            9281..9448
                     /locus_tag="PC545_001461"
     CDS             9281..9448
                     /locus_tag="PC545_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456233.1"
                     /translation="MNWKELWMTLFGTTEWLGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKKDTKESYK"
     gene            10499..10798
                     /locus_tag="PC545_001462"
     CDS             10499..10798
                     /locus_tag="PC545_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456234.1"
                     /translation="MKHLHIQQLSTIGSSLKKHLNSTEHNAPICGFLQHITGIICLAQ
                     DQYVCIALVLFYCIKLFQKIFAVGYQIPPFMVSVSAKRGRSLFALATFAGKGVRL"
     gene            10795..11211
                     /locus_tag="PC545_001463"
     CDS             10795..11211
                     /locus_tag="PC545_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2456235.1"
                     /translation="MKPSSFEGAIRLQFDCLARKVVGRTVKNYDRELGRRARRETPFC
                     DLSEMELNQIGAMDEYCVEFTSFDVFGSEVHIYDERLCEAIKELTESRRNILLMSYFL
                     EMTDAEIAEVMEMERFSVCRNRLHTLKLIKDMYEEE"
     gene            11216..11455
                     /locus_tag="PC545_001464"
     CDS             11216..11455
                     /locus_tag="PC545_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002592280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456236.1"
                     /translation="MMKSTKKCPSFSTISSAADGNEVAIEKILNHYDAYISKASLRPF
                     YDEHGNMYIVLDMELKGRIRAALIKVILGFEVKVK"
     gene            11774..11917
                     /locus_tag="PC545_001465"
     CDS             11774..11917
                     /locus_tag="PC545_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456237.1"
                     /translation="MNNIIRYSMQIAMLKQLLARKLISEKEYTLVKQRLMKDYKVLSD
                     ITA"
     gene            11980..13740
                     /locus_tag="PC545_001466"
     CDS             11980..13740
                     /locus_tag="PC545_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDF2456238.1"
                     /translation="MQEVEVIKAKNVLARQARGKAIERLRVAAYCRVSTDSEDQLNSY
                     KSQVQYYTDMIKKNKEWVLADIYADEAITGTQVTKREDFQRMINDCMNGEIDMVFTKS
                     ISRFARNTLDTLKYVRMLKERNIAVYFEDEKINTLTMDGELLLVVLSSVAQQEVENIS
                     ANVKKGLKMKMKRGELVGFQGCIGYDYHPEDKTITVNQEEAEIVRYIFNRYVDGAGGS
                     VIGQELESLGYKTKYGSSTWAPSTVIGIIKNEKYKGDILLGKTFTVDPISKRRLENMG
                     EEDKFYMKDHHEPIISEEVFEKAQEILNRRNKNRTTVGSGKREKYSRKYAFSCMLECG
                     FCGGTLTRRNWHSGSKYSKVIWQCVTATKKGKKFCKHSKGIPETAIEEAFVESYRLLC
                     DDNKDVLEEFLQRMDDTLSSSVVSKQLAKAEKEIDALEKKKSKLVDMRLEEIIDKETY
                     ESKYADLVSKQEQLVEERQKLQETSDNEKDIKKRLKEFKKTLEQNEVLDKFDRYVFES
                     IVEKVIVGGLDENGNVDPAQLTFVYKTGLKNSVDGAKFKTQRKNARGRHRTNELCSHD
                     SNEVDKMCSDSSNDTCGNCS"
     gene            complement(14229..14867)
                     /locus_tag="PC545_001467"
     CDS             complement(14229..14867)
                     /locus_tag="PC545_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456239.1"
                     /translation="MSNQRKDFDITNDMFQESIPITTKIILEDMPSNDELNHVFSKGC
                     ERKMKKRKIVLITLLLIGVLLLGSILYNLFLGKTANISMLKESWNFDIPIPNKEIEVF
                     DTQDSINGDGQSYFIQGFSEKNFKKVFNLKGGIVVSKDNINEIEKYIDKFKRDSVNIN
                     KSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYSLIWNQ"
     gene            complement(15277..15453)
                     /locus_tag="PC545_001468"
     CDS             complement(15277..15453)
                     /locus_tag="PC545_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456240.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(15507..15603)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16400..16801
                     /locus_tag="PC545_001469"
     CDS             16400..16801
                     /locus_tag="PC545_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HDF2456241.1"
                     /translation="MNYKNRKRAYYQENSCMDNFICKNCGCLVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNS
                     VKLMSIALKPLAQPPFPLERIIEKLEKEETE"
     gene            16798..17865
                     /gene="rsgA"
                     /locus_tag="PC545_001470"
     CDS             16798..17865
                     /gene="rsgA"
                     /locus_tag="PC545_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2456242.1"
                     /translation="MNGMKQINLQDYGLNRNFITQSMEYHDLYLARIISQEKNFYQAV
                     CVNGVLFAEVSGKLRFEATIQSDFPTVGDFVMLDRCNNKNGNAIIHHVLHRKSLFVRK
                     AAGKVNEEQVVAANIDIVFLCMSLNKDFNIHRLERYLSLTWESGAIPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSSIEPDGYKQISKYIKPGCTVALLGSSGVGKSSLINC
                     LMGGEFLNTKEIRIDDDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGLDKS
                     FADIEAYFGKCHFRNCTHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKKIRR"
     gene            18401..19099
                     /locus_tag="PC545_001471"
     CDS             18401..19099
                     /locus_tag="PC545_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456243.1"
                     /translation="MKISSQYRSQYSFRYESNINNTRINESMVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKYRQEVYTKKINDAIKNLCKSEIRETTNNFSNIEIGIKNSIIEE
                     KNKSTMLDENSTYLNTNDEKESLITKESNEKIEEEILNDEKLEELEQKKDYKEDSNKK
                     EKVSEDLSLVGKTREELENMLKNFINLTQEEIMKLESRIEKLDKNAEEYKQNSKTNIF
                     DKTDEQKKHINVLI"
     gene            19277..20167
                     /locus_tag="PC545_001472"
     CDS             19277..20167
                     /locus_tag="PC545_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456244.1"
                     /translation="MKKNILTTMITMIIILMMSMTVMAEPYQEDETSSLKKQSANERN
                     QTNIGTMDGRVQTNAISKGANYDPKYPLKGMLTQLGLNTTGKEGWDLLYGGNPNGVEL
                     RADGKYYYINKGMEGLGFYAAALSGGSYQRIGFGKLDPIEVIADGNWISYKQAQDTLT
                     VIRNFLNSFDWRNASEMERANRAAKLVTEAKYADSKYCNIVYGNLVDKRGVCGSFASS
                     FHLLTRLMGMDSLSILNPSLNHAWNYIQIDGKWYRFDGSEISAFGGSLDFDYRKLKDA
                     TREMTTYYDAKALSILGFNQ"
     gene            complement(20560..21516)
                     /locus_tag="PC545_001473"
     CDS             complement(20560..21516)
                     /locus_tag="PC545_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2456245.1"
                     /translation="MENNTIQKQLRDLSKKIWHIYILNPEEENLKFIISLLDDNLALI
                     GTGKHEFYETLEEFIIGLEKDQTESEQIDFEIIDEWYSCTKVTDEAFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVTDDMIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHV
                     RGDEILIHFSNILTKVFVNDAIIGRLGGDEFAVFEYSNQSKENVENKAKKVLELCLES
                     NSCSIGISKLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            22219..23214
                     /locus_tag="PC545_001474"
     CDS             22219..23214
                     /locus_tag="PC545_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2456246.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFMGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAVV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSDYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAIAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEKLFNYFQKYKKITVSFSNETNIVFPKDCKVVEQT
                     NHKISVIVPKERGNETIKWIFDKYDVKDVMIEEEDIGSIVERIYKKGSDEYYEQL"
     gene            23192..24055
                     /locus_tag="PC545_001475"
     CDS             23192..24055
                     /locus_tag="PC545_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2456247.1"
                     /translation="MSIMSNYNEASSNDSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMVFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLAGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKIDFAFT
                     SNPVQILISILMIFLGFIFMMKLHFFIGILSFKFLEISFLKMIIENIVEFITGTMIPL
                     VLLPETIVTVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            24052..24846
                     /locus_tag="PC545_001476"
     CDS             24052..24846
                     /locus_tag="PC545_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HDF2456248.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPLDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVMIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGIFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFLFTLFMWRFWKLGMRHYKSASS"
     gene            25131..26510
                     /locus_tag="PC545_001477"
     CDS             25131..26510
                     /locus_tag="PC545_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2456249.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCRSYLFIILVFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGLLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLIGEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNILNINGIWLAVPSAEILTLFLTVFCIF
                     KYKNRYKYM"
     gene            26958..27671
                     /locus_tag="PC545_001478"
     CDS             26958..27671
                     /locus_tag="PC545_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456250.1"
                     /translation="MDTCFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIAFRVKAQLRRQQYQSIVPSDSEVIKIGDITIDIEGNRVYKDRNEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(27943..29481)
                     /locus_tag="PC545_001479"
     CDS             complement(27943..29481)
                     /locus_tag="PC545_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2456251.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVVPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQKSAISENEQYLIKASDYQEQLIASNFYSIDNEINKIANGLGLDSIGMNRVVREL
                     SGGQRAKVILAKLLLSNHDVLLLDEPTNFLDKEHVEWLSNYLNTFNGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKSHLREDYIRRYQAQQKKIEKEETFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPSFTGKPNIQFSEIEISAQNALTITNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPRISGDFQFSEQVKIGYYEQDL
                     KWENPDKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLSPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            30363..30818
                     /locus_tag="PC545_001480"
     CDS             30363..30818
                     /locus_tag="PC545_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2456252.1"
                     /translation="MDEIDLKIINSLKKNSRASTSEISRQINLSVPAVSERIRKIEQS
                     NLIQKYTIQINREKTKYKLLAFIFINTDLSKSMKEFKKTILEYDTVLECHHISGEYDY
                     LIKVLAEDTSNLEEFISVTLKEIGGIQKANTTIVLSTIKEEFNTIKDIF"
     gene            30867..31451
                     /locus_tag="PC545_001481"
     CDS             30867..31451
                     /locus_tag="PC545_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDF2456253.1"
                     /translation="MKVLVFLAKGFETMEFSVFVDVMGWARNDYGHDIDVVTCGFKKQ
                     VMSTFNIQVLVDKTIEEVCVDDYDALAIPGGFEEFGFYDEAYDSSFLNLIREFNSKEK
                     IIASICVAALPVGKSGVLKNRKATTYHLKNGKRQRQLSEFDVNVVNEPIVVDKNIITS
                     YCPETAPHVAFKLLEMLTSKEQMDEVKLAMGFKL"
     gene            complement(31456..31779)
                     /locus_tag="PC545_001482"
     CDS             complement(31456..31779)
                     /locus_tag="PC545_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456254.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLENL"
     gene            31931..32449
                     /locus_tag="PC545_001483"
     CDS             31931..32449
                     /locus_tag="PC545_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2456255.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLEKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGINKLSKAANIYDAPLAILVCGDKDKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKIPESPERHEKTR
                     VPLSEIVSYETL"
     gene            complement(32625..33293)
                     /locus_tag="PC545_001484"
     CDS             complement(32625..33293)
                     /locus_tag="PC545_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDF2456256.1"
                     /translation="MLEVSHKVKEIYPNIKFGVMVINITHSKPNKENFLVLKNSTIKN
                     IIEQHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKMELPLKIELANGSEHFYGIGGKEHILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKHYISSAFPDLKVNS
                     IDIF"
     gene            33408..34823
                     /locus_tag="PC545_001485"
     CDS             33408..34823
                     /locus_tag="PC545_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2456257.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILDGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNDTFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRVEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGIKVDDKGMNTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKCATENDYYII
                     EDDYDSEFRYEGQPISSLYELSPNKVIYIGSFSKILSPAIRVGFMLLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIENILEYDYEIILGYAHLS
                     FDEIEKGVKILSKIIHMNQGT"
     gene            35046..35504
                     /locus_tag="PC545_001486"
     CDS             35046..35504
                     /locus_tag="PC545_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456258.1"
                     /translation="MEIIDYIEENLTENLNLDKIAYIANYSKYHLHRMFTNVVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITVGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKCISYANMNYKQRRDEINSYLNFNINCEVKVA"
     gene            35501..36085
                     /gene="clpP"
                     /locus_tag="PC545_001487"
     CDS             35501..36085
                     /gene="clpP"
                     /locus_tag="PC545_001487"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDF2456259.1"
                     /translation="MSLVPHVIEQTGQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSATSGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRCALPNSEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDKILSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            36397..37473
                     /locus_tag="PC545_001488"
     CDS             36397..37473
                     /locus_tag="PC545_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2456260.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGNPTLSYYRERLFKRSSNHKWVDPIHEVVPLFGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKTLEPRHQFYYSRELYYNARYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPSIQLSVCYDRLGESDKAIYYHEK
                     TKEIKPNDSAVLHNENYFSKFKNS"
     gene            37540..>38870
                     /gene="bclA3"
                     /locus_tag="PC545_001489"
     CDS             37540..>38870
                     /gene="bclA3"
                     /locus_tag="PC545_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HDF2456261.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNNGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLR
                     GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADGVTGPTGPTGATGADGITGP
                     TGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGIT
                     GPTGATGATGADGATGVTGPTGPTGATGADGVTGPTGATGATGIGITGPTGPTGATGI
                     GITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADGATGVTGPTGA
                     TGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADGATG
                     PTGATGNTGADGVAGPTGATGATGNTGADGATGPTGATGATGADGATGPTGA"
BASE COUNT        13489 a         5311 c         7139 g        12931 t
ORIGIN      
        1 acccgatagg atatacagac ctaagtagta gttactggca acagcactta atcggtgcag
       61 gacgagtaaa acaatagaaa ggacttgaaa aatatgttta agaagaataa gaaacagaca
      121 gaaaatgtca aagaaaaaaa ggtacgtact gtcaaggtag gcacacataa gaaaaccgtg
      181 attgcattgt gggtggtgct tattgcaagc gtgagctttg gagtatataa gaattttacc
      241 gctattgacc agcacacgac ccatgaaaaa gaagtgattg agcttcgctt gcaggacacc
      301 aacgggatag aaaatttcgt gaagaatttt gcaaagtcct attacacatg gagtaacagc
      361 aaagaagcga ttgacgcaag gacgcaggca atcagcggtt atctgacaaa ggaattgcag
      421 gacttgaata tggataccat tagaacggat ataccgacca gctctgccat tacagatgtg
      481 attgtatgga gcattgaaca gtctggaacg gacacttttt ccgctaccta cgaagtagat
      541 cagcagataa aagaggggga acagacaaca gcggttaagg caacctatac cgtaaaagtt
      601 catgtggacg ctggcgggaa tatggtaatt attcagaacc ctacccttgc accagcaatc
      661 gagaaatcag actatgaacc aaagacacca gaagcagacg caagtgtaga cgcggatacc
      721 ataaatgacg ctactgcatt tctggaaact ttctttaagc tatacccaac agccacagaa
      781 aaggagcttg cttactatgt gtctggaaat gtgcttgagt ctatcggcag ggactacctc
      841 tattctgaat tggtaaaccc tatctttacg gttgatgggg acaatgtgaa agtcaaggtt
      901 gccgtgaaat tcattgataa tcagacaaag gcaacacagg tatcgcagtt tgaattagtg
      961 ctacataagg agagcaattg gaagattata ggataattat tgaaagcgtg catttcttta
     1021 ggaatttgca cgcttttgtt aagagcatta ttctttatat ttatgaaaga catcttaaac
     1081 aaagtattaa tggcttcgca aaactttttt cttaatatat ttggattaga aaaagcgcat
     1141 cttaattgta gacttactat acatatgaat tatgccagtc aacgtattta aaatttatgt
     1201 tgttttattt tgttgtaaat cgcaagaata gataagagta atcaatggtg aaggggaatt
     1261 attaaaaata ctattggttt cttctgtccc gttccaattt gcgcattgta ttttttcata
     1321 aattacagag tggtcaatta tgtagttcag atattccata caaactaatt gtattgtttt
     1381 tacactcggt ttccatactg ccacttttgt cattctatca ctcataatac aaatattatt
     1441 atgagcaact tttctaagta gttcatttaa atttgataca tggtcaaggt gaaaaccaag
     1501 aagaagtttg tcagcactca tatgtgtcaa ggggaaatgc ctatttatgt gcgagagcgc
     1561 tatgagaaaa tgcttgccgt atccatccgt gctccaaatt tagaaggaat ggttgaaaac
     1621 tttgataagg atttatctcg ccctaacgag acggatttga tcgagctgaa aaatgccata
     1681 tcaaagtaaa ttgcaagaaa aaagcaagaa tataaatata gcattttcag aacactgtgt
     1741 taaaatgtta ttacttatac ttataaagga ggggatgttt cgtgtcaaaa gcaacactat
     1801 ttgatagtcg tatcaaaaaa tacgcatact gtagaacatc agaaatttat cgagacattg
     1861 gctcatctcc ggatggttta tccattgagc aaatcggttc aatgcgggag aaatacggag
     1921 cgaacagctt caatgggcgg aaaaatgata cgacgatgca acggttgaga cgggcattta
     1981 ttaacccgtt tcatgtgatt ctttttgttc tgggcattgt ttctcttgtt acggatgttt
     2041 tcgtagcatc caattttgcg agaaatgcta ctaccgccat tatcatcttt tccatgatcg
     2101 taattagtgg cgtaattcgt ctgattcagg agctgcgagc aaagagcgct gctgctcagc
     2161 ttgatcgcct agttcatgaa agcgtcacag tgaggcgtga tggaaagtta atagagattc
     2221 ctggcgaaga attagtggta ggggatattg tactattctc tgcaggtgat cgtgttccgg
     2281 cggatatcag attgacgaag attacggatc tctttatctc tcaggctgcg attactggcg
     2341 agagcgccat cattgaaaag agctgccgaa aactcagtta cggagagcag gatacactga
     2401 ctcagcttga aaacctcgct tttatggcaa ccacagtcat cagtggaaaa ggggaaggca
     2461 ttgtactggc agtaggcaaa gataccctgt atggaggatt taccaaaccg gattctgatg
     2521 ataaaaattc ctttaaaaaa ggagccgatt ccatcgcctg ggtgatgata cgttttatgg
     2581 cgatattagt tcccattgta tttatattac tggggattac tgatggaaaa tggatggaat
     2641 cttttgcttt cgccctgtca gttgctgtag gtctgatgcc ggaaatgctt cctatggtta
     2701 ttacagcctg ccttgccaaa ggaagtcttg ctatgagcaa aaagcagacc attatcaagg
     2761 atattaatgc catgcagagc tttggtagca tggatgtgct ttgtatggat aaaacaggaa
     2821 cacttaccaa tgaaagcatc ctgctggaat attatatgga tatttttggg aatgaaaatg
     2881 cagaggttct tgatcttgct tatctgaata gctgctatca ttctggtgtc tgcaatacca
     2941 ttgataatgc cattcttgcc tgcaatacca tgccagggcg ggaagcccat tacgcaaatc
     3001 ttctgacaaa attccaaaaa gctgatgaaa ttccctttga ctatgctcgt aagtttgtca
     3061 gtacacttgt acaggatagc aatgggaata gtcagcttat tatgaaaggt gatattgaac
     3121 atatattctc acgctgcaac catgtagaat atcgtggaaa acttctcccc atggggcagg
     3181 atgcgagaca gagtgtatct tctgtagtag gtgaaatgtt acaggacgga atgaaggtca
     3241 ttgcggtggc acgaaaaaat gtgggagcgc aaaagaaaat aactcctgat gacgaatggg
     3301 gcatgactct agttggctat ctatccttct ttgatgcacc aaagcagaca gcaaaggagt
     3361 ctgtaactgc actcaaaaga ttaaaagtta tccctaagat tctgacaggg gaccaggcag
     3421 cgattgctgt gtctgtttgc cgccgggtgg gtatttatac agagcgtatc ttaactggta
     3481 ctcaactgga tgaaatgaca gactgtgaac ttaaaaaggc tgtggaacat actcatgttt
     3541 ttgcagagct tactccggga cataaggtgc gtcttgtctc tgctatgcag gataatggtc
     3601 attccgtggg atttcttggt gatggagtca acgacattcc ggcactgaat gaagcaaatg
     3661 tgggaatctc tgtagatacg gcagtggacg cagcaaaaga tgctgccgat gtggttttgc
     3721 tgcaaaagga cttgaatgtt ttggagcaag gtgtaatgga ggggcgaaaa acttttacca
     3781 atatgctgaa atatattaaa atcaccgcta gctctaactt tggaaatatt ttttccatca
     3841 tttgtgccag tgccttttta ccgttcctgc ctatgacttc aattcagatt ctgcttttga
     3901 atctgctgta tgatacgttg tgtattgtgc ttccttggga caatgtagat gaggaagaga
     3961 tgctgtcccc aagagactgg tccggcaaaa cactgagaca gtttatgctg tctttcggac
     4021 cgatcagttc tctgtttgat attgtcacct tcctattttt gtattatttc ctatgtccga
     4081 tgctatgtgg aggggcaacc tatctgcagc ttacagatcc ttccctgcaa cttcagtatg
     4141 tagcactgtt tcagactgga tggtttttgg aatctatgtg gacacaggtt ttgattttac
     4201 attttctgcg aacttccaag attccttttt tccagagcag ggcatctgca ccggtcattt
     4261 ctataactct agtggggatt attgctttta cttcccttac atttgtcggc ggtgcgtcgt
     4321 tgtttggact gacgaaatta cctctttggt atttcgtatt tttactgctg gtggtatctc
     4381 tctatatgct gctgaattca gtagccaaat atttttataa gaaaaaatat caagagttga
     4441 tttaagagga ggcattcatg atgaaaaaga atattagtta tattccgatg gattcggctc
     4501 ttgaaatctg gctattagaa aaactactaa gagatccacc ggacagtgat tgtgcgaagg
     4561 atttactttc tggtattcag gagatgattc gtggagaggt atcttcgtta ttgttaggag
     4621 catcagaaga aagcgattgg ccagtgggta ctctgtcaga tagaatcctg gcaggggatc
     4681 tggaaattta tccaagaggc agaaaagtac tgataaaagg ttctgaagtc aacctgactc
     4741 caaaggaatt tgatattttg tactttcttg tccagaacaa aggtgaagtt tttacaaagg
     4801 aacagattta tcgtgccgta tgggaggaag attatattct ggataacagc aacattatgg
     4861 cttttatccg gaagttgcga aaaaagattg aacctgctcc ggattgtccc acatatattt
     4921 tgaccatctg gggtattgga tacaaattta atgatcagat atgacaaaga cggactaaga
     4981 ccattttcgg tttcagtccg tctattttct taccaaatcg caagaaaatc gcaagacttt
     5041 agccatagga ttgtatttgg attttttata tactgttagt agttaaaggg aggtgtttca
     5101 tatgttttac acagatttta ttttgagaat tgccctctcc ttaacacttg gatttcttat
     5161 tggattagaa cggcaattga ctggtcatcc tgcaggtatt cgtatcaatg tgttaatctg
     5221 catgggtaca agtttcttta ccttgtttcc tatgctctac ggttcggatc aggtattccg
     5281 tgtcggaagc agtattatat ccggtgttgg ttttttatgc agtggtgtta ttttcaagga
     5341 cagagggaca gtacgtggaa tgaataccgc tgccacctta tggtgtactg cagcgattgg
     5401 aattcttgca agtacgggaa tgtatgcaat ggcgatcacc gccgctacta ttttaattgg
     5461 ttccaacctg attctacgtc cctttgcaag aaagtttcac ccaattactt ccagtgatga
     5521 atctgaaaag cagtatcgta tttcggttac atgtcaggaa aaggcagagc aggatatacg
     5581 cttattgctg attaacagca attcctgtaa gacgctcttt ttgaacaatc tggaaagcgg
     5641 tgatgtggtt ggtgacaagg ttgagattgt cgcagaatac tgttctgtcg gaaaatccaa
     5701 aaacaatatt ttagaaggaa tcgttgggca ggtattggta attccggaag tgattagtgc
     5761 tggttgggag gtgttgtgag tgaggagaga aatcgaagat ttgaaacgag caattctttt
     5821 tgctttctac cctgtattta caatcttatg tagctatttg ggttggaatt tctttgaaca
     5881 aagaatggta atctttaaga tatggtatca ggtgagtatt gtgatctggt ttattcttgc
     5941 tgttactatt attgtgctct tcattaaact tctgaagctg acaaaactaa gaaacaggtt
     6001 taagcattga aaggaggaac aaacgatgga ttttacatgt atcttttttg gaattttatt
     6061 tactcttgca ggcttttggt tggcctttgg aaaaggatat cgtcatcttt ccctgtggaa
     6121 aaatatgccg caggaggaga aagataaaat taatattgtc ccgctttgtc gtaatgttgg
     6181 agaaatgatt gcgttaaatg ggattatatt cttaatgaaa ggatttttgc cgggattttc
     6241 aaaccactgg tttgtatttg caataatcgc atggctgatt gttgccggct ttgatgtctg
     6301 gtatatcgaa aaaagtgtcc gctatcgcaa ccaataatcc acattgaggt ttcacataga
     6361 tattttttta agattgtgag gtgaaagaca tggactctga tgtcagtcca cgaaggcaga
     6421 gaatattata tgagaatttg gagattccgt gtcgctcata taatattcct tgcccctatg
     6481 aaaattcaag gaggtaagac caatgaacaa aaaagaaaat cgtatggcta ttcgtcagac
     6541 tgtagaaaaa gcagtcatcc gtgacgaaca gaaccgccgc attcaatttg cggcaaccaa
     6601 tcctattaaa gaagttttaa aaaaacttca taccacactt cgtggcttag aagtagatga
     6661 agtatccgta agtcgtgcta aatatggtac caataaagtc actcatgaaa aaaagaaatc
     6721 tctggcaaaa cgactggttg gcgcatttat caatccattt accgccattt tattttgtct
     6781 ggctttagta tccacaatta cagatatgat ttttccgtat ttttctctgt ttggcagtgt
     6841 gccagaggac tttgattccc tgactgttgt aatcatttta acaatggtat tcatttccgg
     6901 tacgcttcgt tttgtacagg aatccagaag cggaaatgca gcagagaaat tgttagccat
     6961 gattacgacc acttgtacag ttactcgtag aaactatgaa aaaaaagaac ttcctatgga
     7021 tgatttggtg gtgggagata ttgtgcatct ttctgctgga gatatgattc cggcggatgt
     7081 tcgtattttg gatgcgaagg atttgtttgt cagtcaggca agtcttactg gtgagagtga
     7141 acctattgaa aaaacaccca atgtaagtgc tcagaaagaa agcgtgactg attacacaaa
     7201 cattgctttt atggggagta atgtaatttc tggtagcgca acagctgttg ttgtttgtgt
     7261 tggcgatcat actctgtttg gctctatggc gtccgcagtt gctggagaag cggttgaaac
     7321 aagctttacc aagggtgtca atgccgtttc ttgggtattg attcgtttta tgctcattat
     7381 ggttccgctg gtgttctttg tcaatggtat caccaagggc gattggttgg cggccttctt
     7441 attcggcgtt tctattgctg ttggtttgac accagaaatg ttgccgatga ttgtaacaac
     7501 ctgtcttgcg aaaggcgctg tttccatgag caagaagcag acaatcgtta aaaacctcaa
     7561 ttccattcag aacttcggtg ctattgatat tctctgtact gacaagactg gcacgctgac
     7621 ccaggataag gttgtgctgg aataccatct gaatgtaaat ggcgaggacg atacgcgagt
     7681 actgcgtcac gcttacctca atagttactt ccagactggc tataaaaacc tgatggattt
     7741 ggcaattatc cataagacgg aggaagagga agccgcagat tcgcgtctca ttgacctgtc
     7801 tgaaaactat gtgaaagtag atgagattcc ttttgacttc acacgtcgtc gtctgactac
     7861 agtggtacag gataataagg gtaagaccca gatggtaaca aaaggtgctg tggaagaaat
     7921 gctctctatt tgtgcgtttg cagagtgtaa tggaggagtg cagcctctga ctgatgacgt
     7981 tcgttacaga attctggaga cagtggacaa tcttaacgat aagggattcc gcgtactagc
     8041 tattgcacag aagagtaatc cttccccaat cggcgcattt ggtgttaaag atgagtgtga
     8101 tatggtactg attggatatc tggcattcct tgatcctcca aaagaatcta ctgcggatgc
     8161 tattaaagct ctgaaagacc atggtgttat aaccaaaatt ttgacaggtg acaacgacaa
     8221 agtgactcga actatctgca agcaggtggg attagaggtt cgcaatatgc ttctcggttc
     8281 tgatttggaa caaatgggcg atgcagagct tgcaagagtg gcagagtcta cagatgtatt
     8341 tgcaaagcta acccctgatc agaaagcccg tgtggtttct gttctccgtg aaaatggtca
     8401 tacggtcggt ttcatgggtg acggtattaa tgatgctgca gctatgaaag ccgccgatat
     8461 tggtatttcg gtggataccg ccgtggacgt ggcaaaagaa tctgctgaca ttattctatt
     8521 ggaaaaagat ctgatggttt tggaacaggg tattattgag ggacgaaaga catatgccaa
     8581 catgatcaag tacattaaaa tgactgcttc atccaacttc ggcaatatgt tctcagtgct
     8641 tgctgcatct gctttgctac ctttcttacc aatgatgagt gttcatctta tatttctaaa
     8701 cttaatttat gatttatcct gtactgcaat tccttgggat aatgtggatg aggagtttat
     8761 taccaaacct cgtaaatggg acgcttccag tgttggaagt tttatgatct ggattggacc
     8821 aaccagttct atctttgatt ttacaaccta tatttttatg tactttgtat tttgtccatt
     8881 gttcgtatcg aatggtgtat tatttaatga tttgcctgct catttcagcg gtgcagaact
     8941 ggcaatgctt caggcacagt atatcgggat gttccaagca gggtggttcg tggaatctat
     9001 gtggagccag acattagtca ttcacatgat tcgtactcca aagctacctt ttgttcagag
     9061 tcgtgcatca gctccggtga ctttgctaac aatgactgga attatagttc tgaccattat
     9121 tccgtttact gtgtttggaa ctatgcttgg atttgttgca ttgcctgcag catactttgc
     9181 ttatctgatt ccatgtattc tgctgtatat gactttggca acaagtttaa aaaaagccta
     9241 tgtccgtcat ttcggagaac tgctttaagg aggtggaatg atgaactgga aagaactttg
     9301 gatgaccctt tttggaacta ccgaatggct tggtcttaat atcggctttt gggtggctat
     9361 ggcagttgtg ttgctgattg ttattgtaat gaatgtagta ttttggtcca tgaaaccaaa
     9421 aaaagacaca aaagagtctt ataaataaga tatcagtatg aatgaatcaa gggagatgca
     9481 cagtttttta ttgtggaatc tccctatcat atcaaggcgc attcaattgt ggcaatttag
     9541 tagtaaagta tgtgtttttt tatcattgaa ttacctataa atgctgtata taagtggata
     9601 attgtaattt cgatataaaa attatggtac ttaaaagaaa tttcgttttt tattgtttgt
     9661 ttacgcgtct ataacgaaat cgtgaaataa tattcaaagg tagattttat tatggaatgt
     9721 atgtctgtag aagaagcttc gataaaatga ggagatatct gaaagaagga taaaaaaatt
     9781 atgtaatgga aacagaatcc caaatctatt gtaacaacaa tgacaggtaa tgaatggtta
     9841 cgtagattga taattcctat tcaaattatt atctatctta tattgagtgg tgttttgtaa
     9901 ttacattttt atacaaaaag aatatgttga acgcacaatg ataaatattt tgtattcccc
     9961 aactaataga ataaagtttt tgcttggaaa gtatgttata taggcattat ggtttgtaat
    10021 gatcacgttg acagattata ccgctatata tgaagtcaaa gaacttaaat catttacaag
    10081 tgagataatc tatctaagtc taaaaaactg gatttctcaa tctattgtca atgagtccat
    10141 tacttattat ttgtgttttg caaagaaatg ttttctactc tagtttactt tttagttact
    10201 aaatatcggg aattggtttt gttggactat attgatctga aagtatacaa aatattattc
    10261 cgtggtctgc tgtaacccct ataagtttat tagatgcaca aggattgcta ccttatattt
    10321 caattctact ttgttatttt gcaagactga ttataaatac atactgcttt agagggttta
    10381 gtttagttct ttttgaactt ttgaataaga cgacggttat atagccgccg ttttattttt
    10441 gcctaacgtt ggacaaatta ctggatggtc ttgttttatc ggctctaggg gtagccgtat
    10501 gaaacactta catattcaac aactttcgac catcggtagt tcattaaaaa aacatttaaa
    10561 ttctacggaa cataatgctc ctatatgtgg ttttttacag catataacag gaataatttg
    10621 tttagcacaa gatcagtacg tctgtattgc ccttgtgctt ttttactgca taaaactttt
    10681 tcaaaaaatt tttgcggttg ggtatcaaat cccacctttc atggtgagtg ttagtgcgaa
    10741 aagaggtaga agtcttttcg ctttagcaac tttcgctgga aagggggtga gattatgaaa
    10801 ccatcttctt tcgagggcgc tatcagactt caatttgatt gtctagctcg caaggttgta
    10861 ggacgaactg taaagaacta tgaccgagag cttggcagac gtgcaagacg tgaaacgccg
    10921 ttctgtgatt tatctgaaat ggagttaaac cagatcggtg ccatggacga atattgcgtt
    10981 gagtttacgt catttgatgt atttggttca gaagtccata tctatgatga acgcttatgt
    11041 gaagctatca aagaattaac agaaagccga cgtaacattc tgcttatgtc ctactttctt
    11101 gaaatgaccg acgctgaaat tgcagaggtt atggaaatgg aacgattttc cgtatgtaga
    11161 aacaggctgc acacattaaa gcttattaag gatatgtacg aggaggaata aaaacatgat
    11221 gaagtctaca aaaaagtgtc catcattctc gaccatcagt tctgcagctg acggtaacga
    11281 ggtggcaatt gaaaagattt tgaaccatta tgacgcttac atatcaaaag ccagcctacg
    11341 cccgttctat gacgaacacg ggaatatgta tatcgtgctt gatatggaac tgaaaggcag
    11401 gatacgggca gcgttgatta aagtaatctt agggtttgaa gtcaaagtga agtaaatata
    11461 acagagagta tctaccacct cattttaact cttttccgtt ctttgacaag tgaatacagt
    11521 aatcagatac gtttgatata cgacgagcca gcggactgga acgccacgac cactgaaatg
    11581 gggaaagcag cgagcgatta tgccagtgaa ccgcaagcga tctttggaaa ggttgctgcc
    11641 atgactcgta tatctgaata atgatacacc cgtatgacac ggttcaccca tcagaatggg
    11701 aatggtgaaa gtccagtgga gctgccgaag ccgtctgatt acttccataa ataaaaaagc
    11761 aaggagggtt ttaatgaata atataattag gtatagtatg caaattgcta tgttaaaaca
    11821 actgcttgcc cgtaaactga tttctgaaaa agaatacact cttgtaaaac aaaggctgat
    11881 gaaagactat aaggtgcttt ctgacataac tgcttgaatt gtgtttttta aagtagtaca
    11941 ataacaatat gaaaggaaca caaaataagg aggtgtacta tgcaggaagt tgaagtaata
    12001 aaagcaaaaa atgttttggc aagacaagcc cgtggaaaag ccattgagcg tttgcgtgta
    12061 gctgcatatt gccgtgtaag tacagatagc gaagatcagt taaacagtta taaatcacaa
    12121 gttcagtatt atactgacat gattaagaaa aacaaggaat gggtgttagc tgacatttat
    12181 gctgatgaag caattacggg gacacaagta acaaaacgag aagattttca gcgtatgata
    12241 aatgactgta tgaatggtga aattgacatg gtatttacaa aatccatatc aagatttgcc
    12301 agaaatactt tagacacatt gaaatatgtt cgtatgctaa aagagagaaa tatcgccgtt
    12361 tattttgaag atgaaaaaat caatacactt acaatggatg gagagttatt gcttgttgtt
    12421 cttagttctg ttgcacagca ggaagtcgag aacatttcag ccaatgtaaa aaaagggttg
    12481 aaaatgaaaa tgaagcgcgg cgagctggta ggttttcaag gttgtattgg atatgattat
    12541 cacccagagg ataagacaat aactgtaaat caagaagaag cagagattgt cagatatatt
    12601 ttcaaccgtt atgttgatgg tgctggtggt tctgttattg gacaggagct tgaaagtcta
    12661 ggttataaaa cgaagtatgg gagttccaca tgggcaccat ctacggttat tggaattatt
    12721 aagaatgaga aatacaaggg tgatatactc ttaggcaaaa cttttactgt tgatccgatt
    12781 tccaaacgtc gccttgaaaa catgggtgaa gaagataaat tttatatgaa agatcaccat
    12841 gagccgatta ttagtgaaga agtttttgaa aaagcacagg aaattcttaa tcggagaaat
    12901 aagaaccgaa caacggtagg cagcggtaag cgtgagaagt atagcaggaa atacgcattt
    12961 agctgtatgc tagaatgtgg cttttgtggt ggtactttaa caagacgtaa ttggcacagt
    13021 ggctcaaaat acagcaaggt tatatggcaa tgtgtaacgg ctacgaagaa aggaaagaaa
    13081 ttttgtaaac atagcaaagg tattccagaa acagctattg aagaagcatt tgtagaaagt
    13141 tatcgtttat tatgtgatga taataaagat gttctggaag aatttttgca gagaatggac
    13201 gatactctta gtagtagtgt ggtgtcaaaa caacttgcaa aagcggaaaa ggagatagac
    13261 gcattagaga agaaaaaaag taaacttgtg gatatgcgtc tggaagaaat tattgataag
    13321 gaaacctacg aaagtaaata tgctgattta gtaagtaaac aagaacagct tgttgaagaa
    13381 agacaaaagt tacaagaaac ttctgataat gagaaagaca taaaaaaacg tttgaaagag
    13441 tttaagaaaa ctctggaaca gaatgaggtt cttgataaat ttgaccgcta tgtgtttgaa
    13501 agcattgttg aaaaagtaat tgtaggtgga cttgatgaaa acggaaatgt tgaccctgca
    13561 cagttgacct ttgtttataa aacaggcttg aagaatagcg tagatggtgc taaatttaaa
    13621 acacaaagga aaaatgcaag aggacggcat aggactaatg aattgtgttc acatgacagc
    13681 aacgaggttg ataaaatgtg ttccgatagt agtaacgaca catgtggaaa ctgtagctaa
    13741 gataatcaaa caatagtaat gtttatatag attatatact aataatatat tgtatttgcc
    13801 accgatttgc cactgtaaag cgatttcggt ggcaaattta gtctacaatg ttatcaaaaa
    13861 tctctaaagt tttattttta attctataaa acttattata attgtaattt taaaagaaat
    13921 agtcaatcta cagtagccag attttataca actcattttt ttcatagagt aatttacata
    13981 atctatagtt gaatattatt taatattttt ataattcatt agcaaatata ggcattacaa
    14041 atgttgataa acatgtgaaa gcacatatcc acttataatt atttttttta aaaccataaa
    14101 atcacctctt catttgttgt atgtttttat aacaaatgaa gaggtgattt ccagacaatt
    14161 taaaaggttt ttgtatattg actaaaattt atttttatta tttataattc ttaataatac
    14221 atatgtacct actgattcca tattaaactg tataattttt gttcatcttt ttttataatt
    14281 aaaactacgt agttctcata atttctctta tatatatact tatcatcttt tttaacttct
    14341 aacttatatt ttttaaaatc ttcttcaatt ttatttttat tacttttatt aatgttaaca
    14401 gaatctcttt taaatttatc aatatatttt tctatttcat taatattatc tttgcttaca
    14461 actataccac cttttaaatt aaaaactttt ttgaaatttt tttcagaaaa tccttgaata
    14521 aaataacttt gtccatctcc attaatacta tcttgagtat caaatacttc aatttctttg
    14581 ttaggtattg gtatatcaaa attccaagat tcctttagca ttgaaatatt agctgttttt
    14641 cctaaaaaaa gattatatag gatacttcct aataacaaaa cacctattaa tagtagtgtg
    14701 attaatacaa tttttctctt tttcattttt cgttcacaac cttttgaaaa aacatgattt
    14761 aattcatcat ttgaaggcat gtcttcaaga ataatttttg ttgtgatagg aatgctttct
    14821 tgaaacatat catttgtaat atcaaaatct tttctttgat tactcaatat ccatttcttc
    14881 taatatttct tttgattttt tctagtacat tgaatactct tatatacatt ctcctctttt
    14941 aaaattaaaa gttataaaag aataagttat ataaaaaatt attatatttt ttaatatagc
    15001 ttatttgcaa atttataatt ttgttaatct aatccttcaa actaaaaaat catttatatt
    15061 tacagaattt cattttaaga gatgtttatt ttcacataga taagagaatt taagttctca
    15121 tatttattat ttgatacaaa tttacatctt tattatatgt attttaaata aaaaataaaa
    15181 ttatgaatac tagaaatcta tcttaattag ctttctaaca taaggctatt taaagtagat
    15241 aaaactaaaa agggttctct agaaaaccaa atctatttaa ttatacttac ttactaatac
    15301 catcacttga tgcaaacaag attctaccac attttctccg tactctagta taaaatcatc
    15361 tagttgtgta tctataaaat cacaataatc atatgaacta ccaatataag cagtcaacat
    15421 atgtgtacat ctatccattt tatctctaaa catatatatt attatccccc taaaaaactt
    15481 tcataaaaac aaaaagaata tctctttttg gcaactggct gacataactc atagtttatt
    15541 ttataagcgt tagtccctat agctttgcgt cactaaattt ctctagtttt gccgatttag
    15601 ttttattcta caattacaat aatacaatag aattgcttat tattcaatat acttatttga
    15661 aaattaaata aatttacaaa tatgaacgtt attttatatc aaaaaaaata gactatgcat
    15721 gtagaatata tggaaaaact tgattgtagg ttttgtatga aaagacagtt gttattaatt
    15781 taagtgagat aaattgagca acaggcataa aatgagaaaa caaataatag cagttaattt
    15841 ttattattaa tactaggtga acattaaatt tcaaaatacc actagatttt atagaataag
    15901 aaaatgagac aaacataaat atatgaatat attaaattat acttgaacat acttatattt
    15961 tatgataaaa tttcaataat aattattagt gaaggagtgt cttgcctgat gtcagagtta
    16021 tatataatgg taaccaacaa ataaatagtt ggagacgcat atgggttctg tttaatcaga
    16081 atttttaagt gtatttttga tacatttgat tgttgtgtct tactctgcct ttatagataa
    16141 ctaacattag acaacaaata ataatattac atattacttt gttaaattgt ggtgtagtgg
    16201 gctttaatct gctacacttt tattaatttt acacagtatt attttgaata taattatgtt
    16261 gaaatagaag atactaaatt ttctagtcca caggggtttt ctgtttttgt gtaggtaaat
    16321 atgtatacca cataaaaagg cagatataaa tacctgtgga ctttttttgt agaaaataca
    16381 cgatacggag gaaataaaaa tgaattataa aaatagaaaa cgagcttatt atcaggaaaa
    16441 ttcctgtatg gataacttta tttgcaaaaa ttgtggttgc cttgtagttc ctgaaggagc
    16501 tggtacacaa catagaaatc attgtccaca ttgtttacat agtcttcatg tagatatcat
    16561 tcctggtgac cgtgctgcag attgtgatgc taatatggaa cctattggag tgtgggtgag
    16621 aaaaaatggt gagtgggcaa ttattcatcg ttgtacacgc tgtggacatc ttagttctaa
    16681 tcgtattgca gctgatgata attcagtgaa attaatgtca attgcattaa aaccactggc
    16741 acagccccct ttcccactag aaagaatcat tgaaaagttg gaaaaggagg aaacagaatg
    16801 aatggtatga aacaaatcaa tttacaagac tatggtctaa atagaaattt tattactcaa
    16861 agtatggaat atcatgattt ataccttgct cggattatct cgcaggaaaa gaacttttat
    16921 caagctgtat gtgtaaatgg tgtactgttt gcagaggttt caggaaagct gcgttttgag
    16981 gctactatac aatctgattt tcccacagtt ggtgatttcg ttatgttgga tcgttgcaat
    17041 aataaaaatg gtaatgctat tattcatcat gtgcttcata gaaaaagctt gtttgtgcgt
    17101 aaggctgctg gaaaggtaaa tgaggagcag gtggttgcag ctaatattga cattgttttt
    17161 ttgtgcatgt cactgaataa ggattttaac attcatagat tggagaggta tctgtcttta
    17221 acatgggaaa gtggagctat cccagtagta gtacttacta aatcagactt atgtgacaat
    17281 gtgcatcgta aattacaagt agttaattct atagccttgg gtgtagatgt agttgttacc
    17341 tcatctatag agccagatgg ttataaacag atttctaagt atattaaacc aggctgtact
    17401 gttgccttac ttggttcttc aggagttgga aaatcttcat taattaattg tctgatgggt
    17461 ggagaatttt taaatacaaa ggaaattcgc atagatgatg ataaaggaag acatgccacg
    17521 actagacgag agctattttt aatttcaggt ggtggtattg ttattgacac accaggtatg
    17581 agagagttgg gaatgtggga tgtttcagaa ggtttagata aaagttttgc agatatagaa
    17641 gcttatttcg gaaaatgtca ttttcgtaat tgtactcaca cctcagaacc aggctgtgct
    17701 atctgggaag ctattgctca tcaagaactt tcgcctaaaa gatgggaatc ctatcgtagg
    17761 cttaaaactg aagcagtatt tgcagacaat aaaagcgaat acttagcaaa aaaagaaaag
    17821 aaatttaaaa atatctcaaa aataaataag aagattagaa gataagtgta gtttttttgt
    17881 tcattctatg agtctatata ctattaatat attaaatttg ccatcaattt gttactttaa
    17941 tgtgattttg atggcaaatt tggtttgtgg tattatttaa agcaatttat taattttttt
    18001 aataaagttt gattttttat atgatgatat atacatgtga taatgtatac atatttgtta
    18061 cgataggctc ctttataaag aggtcttatg taagataaga aaaggcatat ttatactaat
    18121 acataataga gtatatgctt atgtatgata gagatgtttc ttaagagcaa aactgcaata
    18181 ttgctaattt ataaagtaaa aatatatgaa ttataaaata caaataatat atttataaaa
    18241 attattgttt taatttataa taaaaatttt ttgttgaaaa taatgctcga acctataaaa
    18301 ctattaacta atatttgtaa atgtatactt ttgatgataa aatataatta agttaagttg
    18361 gtttttataa acataaattt attttaagga ggtgaaataa atgaaaatat ctagtcaata
    18421 tagaagtcaa tattcattta gatatgaaag taatataaat aatacaagaa taaatgaaag
    18481 tatggttaag aaaaatgaaa ctgtaggaaa agacacttat ttatctaata ttatgaaaca
    18541 aaagcaagaa cttaatgata gaattagaga tttaaaatat agacaagagg tttatactaa
    18601 aaaaataaat gacgcaatta agaacttatg taaatcagaa ataagagaaa caactaataa
    18661 tttttctaat atagaaatag gtattaaaaa tagcattata gaagagaaaa ataaaagtac
    18721 aatgttagat gaaaattcaa cttatctaaa tacaaatgat gaaaaagaat ctttaatcac
    18781 taaagagtct aatgaaaaaa ttgaagaaga aatattaaat gatgaaaaat tagaagagtt
    18841 agaacaaaaa aaggattata aagaggattc taataaaaaa gagaaagtat ctgaggactt
    18901 atctttagta ggtaaaactc gtgaagagct tgaaaatatg cttaaaaatt ttataaattt
    18961 aacacaagaa gaaataatga aacttgagtc gagaatagaa aagttagata aaaatgctga
    19021 agaatacaaa caaaattcaa agactaatat atttgataaa acagatgaac aaaaaaaaca
    19081 tataaatgta ctgatttaaa caatatgttt taaaaaaata agtttattat ataatgaaaa
    19141 tataaaggta caatttcaat acacttctat taaaccaata atttatgaat cagatttaat
    19201 ttcaatttac taataaccat atatttatga gttaataatt ttatgtttta aaaagaatta
    19261 aataaggggg agtagtatga agaagaatat attaactact atgattacca tgattattat
    19321 attgatgatg agtatgacag taatggcaga gccatatcaa gaagatgaaa catctagctt
    19381 gaaaaaacag agtgcaaatg aacgtaatca gactaatata ggcacaatgg atggcagagt
    19441 gcaaacaaat gcaataagta aaggtgctaa ttatgaccct aaatatcctc ttaaaggaat
    19501 gttaacacaa cttggtctta atacaacagg taaagaggga tgggatttac tctatggagg
    19561 aaatcccaat ggagttgagc ttcgagcaga tggtaaatat tattatataa ataagggtat
    19621 ggaagggcta ggattttatg cggcagcatt gtcaggtggc tcatatcaga gaataggctt
    19681 tggaaaactt gaccctatag aagtaatagc tgatggaaat tggatttctt acaaacaagc
    19741 tcaagataca cttacagtaa tcaggaattt tttaaatagc tttgactgga gaaatgcatc
    19801 tgagatggaa agagctaata gagctgcaaa attggtaacc gaagcaaaat atgcagactc
    19861 taaatattgt aacatagtct atggtaatct ggttgataaa cgtggtgtat gtggaagttt
    19921 tgcaagtagc tttcatttat taacaaggtt aatgggaatg gattcactat ctatattaaa
    19981 tccatcatta aatcatgctt ggaactatat acaaattgat gggaaatggt ataggtttga
    20041 tgggtctgaa atatcagctt ttggtggatc attagacttt gattaccgta aattaaagga
    20101 tgcaacaaga gagatgacta cttactatga tgctaaggca ttgtctatat taggatttaa
    20161 tcaataaaaa tagggggagg cttcttatag agcctttttt tattgatttg aaatttaata
    20221 aggaatttta tatgaaaatt taatcaataa aagcaaaaaa ggctctttat aaagcctttc
    20281 taagtgtttt tttaagtaaa attttttata tcaacatcag ttttaatcaa aacatataac
    20341 attttggact tacttttaaa aataaaatta ttaaaatata atattaatca tgtttttagt
    20401 ttattatatt ctataatagt ggtcatgttg actattgtag ttttattaag aatagttggt
    20461 ttatataaat atatgcaaag atagcttgtt gtttacttta cttattagta tataggacaa
    20521 gatactctaa taaatttgcc tattttaata tcttttgtac taaaaccaac taaagtttcc
    20581 tttattattt cgttttgaat aatacaaagc ataatccgat tgtttgtata aagactggaa
    20641 agtatcagag ttattttcta atttagatat acctatagaa caagaattac tctctaggca
    20701 taattctaaa accttctttg ccttattttc aacattctct tttgattgat tactatattc
    20761 aaaaacagca aattcatctc caccaagtct tccaataata gcatcattta cgaatacttt
    20821 agtcaaaata tttgaaaaat gtattagtat ttcatctcct cgaacatgac caaagatatc
    20881 atttacctgt ttaaaattat ctaaatcaaa gatgtaaaag tatccatgtg aaacagtttt
    20941 aagcatttct tttatgtgta cttcaaaaaa atgtctattg tatagtccag tcataaggtc
    21001 tttttctgat tttagtttaa attcatttgc cattgctagt gcttcttgtg ctttttcagt
    21061 tattgtttta gggtaatact catctttcat ttggtcaaag tttggaatag aatggtgaac
    21121 atggataagt ttaatcatat catctgtaac tctgtatacc atagtaaatc gagtatccat
    21181 ttctacatag atatcttttt ctaggtaatc tttttctatt acccaaagag taccatatac
    21241 tataaaagct tcgtctgtaa ctttggtaca tgaataccac tcatctataa tctcaaagtc
    21301 tatttgctct gattctgttt ggtctttttc aagacctata ataaattctt ctaatgtttc
    21361 gtaaaattca tgttttcctg ttccaattaa tgcaaggttg tcatctaata aggaaataat
    21421 aaatttaagg ttttcttctt ctggatttaa aatataaata tgccaaattt tttttgataa
    21481 atctcttaat tgtttttgta ttgtattatt ttccatagat taccccctta agatttacag
    21541 tttatatttt aatgtattat aagttgaact cttattacaa tattattata atatagaact
    21601 aaagaaatta catcctgtat aagtaaaagt atatctttta taataaaaat attacagtaa
    21661 aataaccagt tttaatatac gtattatatt tttaatttat aaacgaatat tataaatgaa
    21721 atttaaattc aaccctgaag aaataagcta aatgtattag tatataaatc catacttgaa
    21781 cataaataac ctttatgata tgatatcaat aataatatta tgagaggagt gccttgatgt
    21841 cggagttata taatggtaac caacaattaa atagttggag acacatatat tctgtataat
    21901 cagaaatttc aagtgtattt ttaatacatt taatttttgt gtcttattct gcctttatag
    21961 ataactgaca tatgacaaca aatagtattt aaaatatata ttactttgtt aaattatggt
    22021 gtagtaggct ttaatctgct acacttttat taattttgta tagtattatt ttgataacta
    22081 ttttgaaata gagagcatta atttttctag tccacaggag ttttctgttt ttgtgctgtt
    22141 aaatataagc acagagaaag gcagatatat acctgtggac tttttttata taaattatga
    22201 aatatggagg tagttaaaat gagtataatt gaacttaatg aactgactaa atcttataga
    22261 agatttgaga aaaaggcagg atttatgggg agtgtaaagt ctttatttaa taggaaatat
    22321 acagaaaaaa cagctatatc gtcatttgat ttaaagatag aagaaggaga atttgttgga
    22381 ttaataggtc aaaatggagc tggcaaaact actcttatta agatgttaac tggtattatt
    22441 gcaccgtctt ctggagaaat atctgtaatg ggttattacc ctaacaaatt agaaaataat
    22501 tttaaaaaac aatatgctgt tgttatgggc caaaaaagtc aactattctt tgaattgaca
    22561 ccagcagata cgtttctttt atttaaagaa ttatatgata tatctaatga agaatataag
    22621 aaaaacttgg attactttat cgaattattt gaggtatctg actatttaaa tgttcaagtt
    22681 aggacgcttt ctttaggaga aagaatgaaa atggaactaa tagtagccct tctacataat
    22741 ccaaaaatat tatttttgga tgaaccaact ataggcttag atgctatagc ccaaaaacaa
    22801 attaggtatt tcctaaaaga agtaaatgaa acaaaaggaa ctactattat acttacttct
    22861 cattacatgg aagatattaa gagtctctgt agtagaagta ttgttgtaga aaatagccgt
    22921 aaaatatatg atggttctac agagaaacta tttaattatt ttcaaaaata taaaaaaata
    22981 acagttagtt tttccaatga aactaacatt gtatttccaa aagattgtaa agttgttgaa
    23041 caaacaaatc ataagatatc agtaattgtg ccaaaagaaa gaggaaatga aacgattaaa
    23101 tggatttttg ataaatatga tgttaaagat gtaatgattg aagaagaaga tataggtagt
    23161 attgtagaga ggatatataa gaaaggaagt gatgagtatt atgagcaact ataatgaagc
    23221 aagttcaaat gattcaatgt tttataaatt taaacagagt attagaaggt attatgaagt
    23281 agccaaaatt aactttaaag tgcaacttat ctggagattt gatgtggtgg ttaatatggt
    23341 gtttacgatt gtaaaaattt tatttgctta tattttatgg aaagcaattt ttggtgaaaa
    23401 taaaatagtc tctggtttta catttaactc tatgttatct tactatataa taagctcatt
    23461 tttagcaggt attgatatgt caaagagtac cagtgaagag atatgtaatc gtgtaaaatt
    23521 gggcaccttt tcaaagtaca tgattattcc aattaatgtt caaaattatt ttattgctaa
    23581 aaattttgga atgacatgtt tttattttgt atttaatatg atttctgcat ttgtatggat
    23641 ttttatattt aaaatagatt ttgcctttac atcaaatcca gttcaaatat taatttcaat
    23701 tttgatgatt tttctagggt ttatttttat gatgaaactc cacttcttta ttgggatttt
    23761 gtcatttaaa tttcttgaga ttagtttttt aaaaatgata attgaaaata ttgttgaatt
    23821 tattacagga acaatgatac cacttgtact cttaccagaa actatagtaa cagttatgaa
    23881 ggtatttcca ttttattata caacatattt gccatctatg ttactaatag gtagaaacag
    23941 caatgaggca atacaaggat taattattct agcattatgg gttttatttt tttctgtagt
    24001 aaataaagtt atgtataaaa gaatgagaac attatttgat ggggtgggta tatgatgaaa
    24061 aaaaatttga aaataatatt tatgttatta aaaatgaaat tatcaaagat gatggtattt
    24121 agattcgatt tttttggagc attttttgta gatagttcat tatttatatt acaacttcta
    24181 atgtttaata gtatatattc tcatgtagac tcaatagggg gatggcaaca aggagagatg
    24241 ttaatattta taggaacttt ctcattaata aatgcgataa atatgacgat tttctttttt
    24301 ggtatttatg atattccacg aaaaatccaa gaaggagaat tagactacta tattacaaaa
    24361 ccattagacc cactatttag gttaacattt gaaaatatta atattggttc atcaccatta
    24421 attctagcaa gtatacttat tgttttatat ggaatatctg agttaaactc agagataact
    24481 ttagttatga taataatata tattttcatg gtgttattga tgacattact tttttatgat
    24541 gtatgtatca tacttagaac agtttcattt tttgttatat catcttctgc gataatgaga
    24601 ttagaagata atttattgcc aatgaatatg aaaattcctg gtgtaatata tgaaggtatt
    24661 tttaaagtat tattctattt aatcttacca tatggaatta tgtcgacaat tccgactcaa
    24721 attttggctg ggacaataac aattaatgga ttaatatact caattttaat tgtatttttg
    24781 tttacattgt ttatgtggag attttggaag ttaggtatga gacattataa aagtgcaagt
    24841 agttaataaa ttagtaaaaa tagacaatgt ataactatat ataaatacgt ttcatatgac
    24901 actaaactat gtgaacaagt atttgcgcta aaggcactga aagctataaa atgattaaag
    24961 taaggattta aaagtttctt tagtagaaat ataattgtgt aaaaaagcaa gattaattaa
    25021 aactatatcc aaataattag atataattct tatgcagaaa ggaggtctgt aaatgtctaa
    25081 ttgtttggaa tatattttaa aaataattaa tgtacagaaa aataaacaaa atgagtgatg
    25141 tatattattc agaaaatcat gaacaagata atctttatga gcaagataat ttactggcac
    25201 aaagatttaa tttatttact ctattgaagt ttgcatttcc tactattttt atgatgattt
    25261 ttatggggtt gtatacaatc gtagatacta tatttgtatc aagatttatt aatacaaatg
    25321 ctctatcagc aattaatatt gtatgtccaa ttattaatct tatagttgga attggaacta
    25381 tgcttgccac tggaggaagt gctattgtag cacgtaagat gggaaatggt gatattgagg
    25441 gagcaaataa agctttttct ttaatcgtat tatcaggagt tgtaattggg gtaataatta
    25501 ctggtatagg tattgtattt atggattata tcattatatg gttaggagca agtgaagttt
    25561 taattaaata ttgtcgttca tatcttttta ttatcttggt ttttgcttct gcaaatatac
    25621 tacaagtatt atttcaatgt ttatttgtaa cagctggaaa gcctcaattt ggattatggc
    25681 tagtgttagg tgctgggtgt gctaatataa ttcttgacta tgtgttcatt gttctgttac
    25741 aaatgggtat tagtggagca gcacttgcaa ctggagtagg atacctaatt cctaccattt
    25801 caggtatggt tttcttttca aaaaataaag ggttacttaa attttgtata cctagactag
    25861 ataaaagaat attatttgaa gcttgtttta atggttcatc tgaaatggtg ggtcaactat
    25921 caacagcagt tactactttt ttatttaatg ctatgatgat gaaattaatt ggtgaagatg
    25981 gggttgcagc tattacaata attatttatt ctcaatttct tttaacttcg ctttatatag
    26041 gtttctcaat gggtgttgca ccaataataa gttataacta tggaagtgaa aataatacgc
    26101 agttaaatca tgtttttaag atttgtatat tatttatttc agtagcatct ttattggtat
    26161 ttatcttttc tatatcatgt tcaccaatca ttgtagagat tttttctcaa agaggaagcc
    26221 gagtttatga aattgcaagg tccggatttt atatattttc ttttagtttt attttttgtg
    26281 gatataatat ttttacctca tctatgttta cagcattatc aaatggaaag gtgtctgcaa
    26341 tattatcttt tctacgtaca tttggattta taactatagg tttattgata ttgccaaata
    26401 tattaaatat aaatgggata tggttagcag taccaagtgc tgagatttta accttatttt
    26461 taactgtttt ttgtatattt aagtataaga atagatataa atatatgtag atattaaata
    26521 aatagcttag atagatattt tatgttttaa taagtaattc aattagtggt agatatatac
    26581 tattgaaaat taaagttata attgatataa agaattaata aaaataacat ataaaagttt
    26641 aaaagggagt tgaacttaaa gtttgattag aaaaagtcaa ttttaagaca actcctttta
    26701 ttttactatt gtattaatct gtatctgtaa gtgtattagt ctaatattat aaactttgga
    26761 tgaaattaat tttctatagg ttttaatgta tcatacactt tattttaaag aaacttatct
    26821 taattttcat agtgattgaa tttgtctatt agagatgtta ctattagtaa tgtaaaagca
    26881 agagtagtga atataaaaaa caagatagtg atactgcaag attagaatat aataaatttt
    26941 tatggagaga tttcaatatg gatacatgtt ttaataaaaa aatattactt gttgatgatg
    27001 aaaaggatat agtagattta attgaagaag tactcataaa tgatggtttt aaaaacatta
    27061 ttaaagctta taatggtcta gatgctattt cgctgtgtaa gatagcatgt ccagatgttg
    27121 tcattcttga tataatgtta ccagatatag atggtataga agtgtgtaaa aaaattcgag
    27181 agttttcata ctgttctatt ctttttttat catcaaaaaa tgatgatatt gacaaaatac
    27241 taggtcttag tagtggggga gatgactata taacaaagcc attcagtcca agagaaattg
    27301 cttttcgagt taaagcgcaa ttacgtagac aacagtatca aagcattgta ccatcagata
    27361 gtgaagttat taaaatagga gatattacta ttgatataga aggaaaccgt gtatataaag
    27421 atagaaatga gattgagtta actggaaggg agtatcatct tttaagctac atggctcaaa
    27481 atgtaaataa aattatagga aaagaaagac tctatgaaca agtatgggga gtatatagta
    27541 gtatttgtga caatacaatt atggtgcata tacgacatat aagagaaaaa attgaagata
    27601 acccctcaaa tcctaaaata cttataacag ttaaaggact aggatataaa cttgtgaata
    27661 gaatcgatta gaatagattt taatattaat gaagatatct caagttgaat ttttagttaa
    27721 tgaggcattc ttttatgggt gaaatcaaat gtattttcgt tatctcaaca atgaagaaga
    27781 ggtctatctc tattttgaga tagcctcatt aagtttatat cctggtaatg tactttatgt
    27841 tctaataaaa tttttttagt ttatttgcag tgaaacatta actagaacat taaaatttgc
    27901 attgaaatac caattaaata atattgtata ttgtgtataa acttatacta attgtttttc
    27961 tatattaaat actttatcaa tccaaccttt ataaaatttt tcttcatggg aaactaggat
    28021 tatacttcct ctaaactgtt tcaaagcatt ttgtaaagca tcctttgtct ctgcatcaaa
    28081 gtgattggtt ggttcatcca gaataagaaa attacaagga gataacatca tacaacataa
    28141 tttaacctta gattgttcac caccacttaa agtagataca ctccttgaaa catgctcttc
    28201 cttcacacca catctggcta gatgacgtct aatctctttt gtatttaatt ttggatattt
    28261 atctgctact atttgaagtg gggttttatc aggattttcc cattttaaat cttgctcata
    28321 ataaccaatt ttaacttgtt ctgaaaactg gaaatctcca gatatacgag gaatatcttt
    28381 gactaatgtc tttaacaagg tagatttacc gattccatta aaaccagtta taactatttt
    28441 ttgacctcca tcaacagaga aatttaattt tggaagtaaa gaatagtagt atccaacttc
    28501 aagatttgtt atagtaagag cattttgagc cgatatttca atttctgaaa attgaatatt
    28561 tggttttcct gtaaaactag gaggagctat tctttctatt ttatccaatt gtttttgtct
    28621 tcctcttgca atttttgaat ttactccagc tttattttta cgtataaatg tttcttcttt
    28681 ttctattttc ttttgttgtg cttgatacct tctaatataa tcttctctta aatgtgattt
    28741 ttgtttaagg aactctgaat attttccatg atatttctta atcatgccaa attctatatc
    28801 aagaatacct gtagaaattt tttctaaaaa gtcaaaatcg tgggaaacta caataaatgc
    28861 cccattaaat gtatttaaat aatttgagag ccattcaaca tgctctttat ctaaaaagtt
    28921 tgttggttcg tctaataata gaacatcatg gttagaaagt agtaattttg ctaaaattac
    28981 ctttgccctt tgaccaccac taagctctct aacaacacga ttcattccta ttgaatctaa
    29041 tccaagacca tttgctattt tatttatttc attatctata gaatagaaat tacttgctat
    29101 tagttgttcc tgataatctg atgctttgat tagatattgc tcattttcag atatagcact
    29161 tttttgatag agtaaattca tttccttttc tattttataa agttcttcaa atgcagtgtg
    29221 taaatagagt gatattgttg tatctctatt gatttctgca tattggtcta aatggcctat
    29281 attgatatta ggttgccact tgatttctcc tgaatctgga acaacttctc ccaataaaat
    29341 tttaattaat gtacttttac ctgtaccatt ttggccaact acacctatat gttcaccttt
    29401 gaataaatca aagttagctt tctcatacag tgatttatcc ataaaacatt gtgataaatt
    29461 agttactttt aataaactca taattcaaac tccttttctt ttattaaaaa atttatggaa
    29521 ataaaaaaag cagaggaatg gcattgtaaa atgccgacct ctgcttttgg aaaaacaaaa
    29581 ggtcatagac aagcattgtt gtctatgacc agaaaatcta tattatctat cggcaaatag
    29641 gtaattttaa ataagcatag ccaaaaaact atactaaacc tatatttgcc gatgaagata
    29701 gaaagcttta agctttcaga ttaacctcta cacaacgctt catcgtatac atttgtgtag
    29761 agttaagttt tctaacaagt ttaactaatt ctatataaat cataattatt cacatcctaa
    29821 tcttagtatt aattctagtt tatctgtatt taaattgtac ttttgtactt tgtggattat
    29881 cttatttatt taaaatatgt tttattaatt atagcttaag ttatgttatc ataatactta
    29941 gttatagtca ataagaaagt tatatttact attattatat taatttagta aatctatcat
    30001 tacaaagtta tttgaagact cgctatcaat aaaattttat agtctataag aaaattaaga
    30061 ttatgagttt tataattttg atatagggat actattttca taaatattac aacatgtaga
    30121 agtatatgtc tattattgtt gaattttcaa tgatatatta aggttctaat atattataca
    30181 aaataaaaac attaattcaa cttaaataat tttaagtatt atatgtgttt tgattaaaga
    30241 aaactaaaat tttaaggaaa aataacatat ttgtattaaa ttttattgaa atgattaaat
    30301 ttatttactt aatgtaaatt attagctaaa attgtattaa ataattaagg aggtgagtag
    30361 ctctggatga aatagattta aaaataatta attctctaaa aaagaatagt agggcttcaa
    30421 cctctgaaat aagcagacaa attaatttat cagtaccagc agtctcagag cgtataagga
    30481 aaattgagca gtcaaattta attcaaaagt acactattca aattaatcgg gaaaaaacaa
    30541 aatataagct tttggctttt atatttatca atactgattt atctaaaagt atgaaagaat
    30601 ttaagaaaac tatattggaa tatgatactg tattagaatg tcatcatatt tcaggagagt
    30661 atgattactt aataaaagta ttagcagaag atacaagtaa tcttgaagag tttatatcag
    30721 tgactttgaa ggaaattggg gggattcaaa aagctaatac aaccattgtt ttatctacaa
    30781 ttaaagaaga atttaataca ataaaagata ttttttaaat aatttactag taaagtgtta
    30841 attatatttt aggaggaaaa tagaatatga aagttttagt ttttttagca aaaggttttg
    30901 aaactatgga atttagtgta tttgtagatg ttatgggatg ggctcgtaac gattatgggc
    30961 atgatattga tgtagtgact tgtggattta aaaaacaagt tatgagcaca tttaatatac
    31021 aagtattagt tgataagacg atagaagaag tatgtgttga tgattatgat gctctagcta
    31081 ttcctggagg atttgaagaa tttggatttt atgatgaagc atatgattca tcttttttaa
    31141 atctaataag ggagtttaat tcaaaagaaa aaataatcgc ttctatttgt gtggcagcat
    31201 tgccagttgg aaaaagtggt gttttaaaaa atcgtaaagc tactacatat catttgaaaa
    31261 atggtaaaag acaaagacag ttaagtgagt ttgatgtaaa tgtagttaat gaaccaattg
    31321 ttgtagataa aaatattata acctcatatt gtccagaaac agctccacat gtagctttta
    31381 agcttttaga aatgttgaca tctaaagaac aaatggatga agttaaatta gcaatgggat
    31441 ttaaactgta atggtttata gattttctaa gtaatcagaa ccccagtcac acatggaatc
    31501 taaaattgga ataatactct ttccaaaatc agatagcgag tattctactt ttggaggaac
    31561 aacaggataa accactctta taattaaacc atctgattct aattcacgta attgttgagt
    31621 taacatctta ggcgttgctt taggaattaa tttttttaat tcattgaacc tcaatatagt
    31681 gtctttaaga tgccaaagta taagtgtctt atatttacca ccaatcaatg caagtgttgc
    31741 ttctattggg caactgtaat tatctaaaca ttttttcatt tttgggctcc tttatttatt
    31801 ttaaagtatc tttttaggta ctatattaca aaaaagtgca ttattgtaat atagtaccat
    31861 agtgataaaa ttatagcata ggttaaataa atcatcaact tagcctaata aaacaatagg
    31921 gggtaaataa atgaatttcg ttgaacttgc aaaaaaaaga tattcttgcc gtaattatca
    31981 agatagaaag gttgaaaaag aaaaacttga gaaggtttta gatgttgcaa gaatagctcc
    32041 aactggtggt aatcgtcaac ctcaaagatt gattgttatt caggaaaaag aaggtataaa
    32101 taaactttct aaggctgcca atatatatga tgcgccatta gcaattcttg tatgtggaga
    32161 taaagacaaa gtttggacaa gaccttttga tgggaaacaa cttacagata ttgacacaag
    32221 tatagttaca gaccatatga tgttacaggc aactgaactt ggattggcaa gtgtttgggt
    32281 gtgctatttt aatccagata taattagaga agagtttagt ttgccagata atctagaacc
    32341 aataaatata ttattgatgg gatatgaatc taagatacca gagagcccag agagacatga
    32401 aaaaactcgt gttcctttaa gtgaaattgt ttcttatgaa actctttaat tataaaatta
    32461 agttattcat aggataaatg taataaagtg tttgttataa acaaaattca aaataaataa
    32521 tatctacata taattaattt tagtgaagta tatctagttt aactagaatg tatacttcac
    32581 taatttattt attcaaaata tttatgagga ttttaatata ttaactaaaa tatatcaatt
    32641 gaattaactt ttaaatctgg aaaagcactt gaaatatagt gttttatatc atttaaatgg
    32701 tcttgaattt gcttttctga tattttatca ggtccatata caaaatacat aatattttta
    32761 gtatcttttg ttatagctgt tcgattatca gggccattaa gaatactaga taatatacca
    32821 atattatctg ataaaaataa atcatctttt gttaaaatat gttctttacc acctatacca
    32881 taaaaatgtt cacttccatt tgctaattct attttcaaag gtaattccat tttatcaagg
    32941 tcatgacctg ctgttagaag aagatttttc aattctgcta aaaacattga ctcaattgtt
    33001 acacctacat ctgggatacc ttttgatttt aatagaatag attctaattg aagcaataca
    33061 gggtaagttt tcttgaattt tttataatac tttatataac ttgaaacagg ctctgtttta
    33121 attttttctt tgcgattgta ttctgggtgt tgttctataa tatttttaat tgtagagttt
    33181 ttaagaacta aaaaattttc tttattaggt tttgaatgtg taatatttat aaccataact
    33241 ccaaatttaa tattgggata aatttcttta actttatgtg atacctctaa cataatatag
    33301 actccttttt attttatatt ttatgatata atcttagcaa attaaaagtg gattttatag
    33361 ttccattttt atagtaattt tatagaacca cttttggagg tattagaatg tggattacga
    33421 ttaatagaaa tagtaatata tcattatcaa gacaaattta ttgtgagata aaagatatga
    33481 ttctggatgg aagcttaaag tttgagcaaa aactgccatc atctaggact ttatcaaaag
    33541 aactatctgt atctcgtaat acagttttag atgcatataa tcaattaatt gcagaaggat
    33601 atttggaaac aaggaatgga tatggaacaa ttgtagctaa aggaatatca gggcataaag
    33661 ttgattttaa gttaaatgat acttttagag atgaaaaaga tttacatact aataaacaaa
    33721 aaaattatat agattttcgt tcaggagttc cagaattaaa actatttcca cgcaaagaac
    33781 tttcaaagct atataaacaa atactgtatg atttatcaga ttcggatttc agatatggaa
    33841 gtacagctgg tgtatgggtt ttaagagtgg aaatatctaa gtatttattt cgtactagag
    33901 gaattagatg taatcccaaa aacattatga ttgtttctgg ttcaacacaa ggtttatctt
    33961 taatttcaca gcttttatac aaaagtaata aaaaggttgc tgtagaagac cctattcata
    34021 aaggcttatt aaaggttatt tcatctattg gatattcagt tgttggaatt aaagtagatg
    34081 ataagggaat gaataccaac ttaataaaat caataaaggg gatttctttt gtttatacta
    34141 caccttctca tcaataccca ttaggaggga ttttacctat tcaaagaaga ctagagctta
    34201 ttaaatgtgc aacagagaat gattactata taattgaaga tgattatgat agtgaattta
    34261 gatatgaggg tcaaccaatt agttctcttt atgaacttag cccaaataaa gtaatttaca
    34321 taggttcttt tagtaaaata ttatctccag ctattcgtgt tggatttatg ttattaccag
    34381 acaatatttt agaaagctat aaaaccttaa aaatgtactc tgatgttcat acagaaacaa
    34441 tattgcaaca tgtattagct gaatttattg gaaatggagg tttagagaag tacatattta
    34501 gaatgaagaa gttgtacaat agaaaacgta agtacctcat aaaagtttta aacacggaat
    34561 ttaaaaatga atttaaaata aaaggtgaag cagcagggct tcatatagtt gctcatttct
    34621 ataatgttaa atttacggaa agtttaattc ataaaatttt tgaagcaggt ataaaagttt
    34681 atcctataca acaatatgca attgagaata tattagaata tgactatgaa attatactag
    34741 gttatgctca tcttagtttt gatgaaattg aaaaaggtgt aaaaatactc agcaaaataa
    34801 tacatatgaa tcaaggtaca tagataaaac atgtagcaat ctacatataa tagatgtaat
    34861 taagtttaaa ctttaaaaga aatatatatt aatatttttg tataaaaata cctgatattt
    34921 ttttatcgat actttattgc tatacactaa attaagatag caatttatat taagaaaaaa
    34981 ctttttgcat aggttataat acttaaaagg agaggataga gttgaaaaca aatacagatg
    35041 ttattatgga gataattgat tatatagaag aaaatcttac agaaaatcta aatttagata
    35101 aaatagctta tattgctaat tattcaaaat accatttaca cagaatgttt acaaatgttg
    35161 taggatgtac tatccatcaa tacatacaga ggaggagact tactgaagca gctagacaac
    35221 ttatatttac agacaaatat attatagaca tagctattac agtaggatat tctacacatc
    35281 aatcttttac actagcattt agaaatttat ataaagaatc tccacaagta tatagaaata
    35341 aagatgaatt ttatcctatt caacagagat ttgttttaaa tctaaatcat caaattcaaa
    35401 gtgaaaaatg tatttcttat gcaaatatga attataaaca aagaagagat gaaatcaatt
    35461 cttatcttaa ctttaatatt aattgtgagg tgaaagtagc atgagtttag tgccacatgt
    35521 aattgaacaa acaggtcaag gggaaaggtc ttatgatata tattcaaaat tattaaaaga
    35581 tagaattata tttataggag aagaaattaa tgatgctatt gcaagtttag tagtagctca
    35641 attactgttt ttagaatcag aagacccaga ttcagatata attatatata taaatagccc
    35701 tggtggctct gctacatcag gatttgctat atatgatact atgaattatg tcagatgtga
    35761 tgtatcgact atttgtattg gtatggctgc aagcatgagt gcttttttac ttgcaggagg
    35821 aacacatgga aaaagatgtg ctttacctaa ttcagagatt atgattcatc agccaatggg
    35881 aggagctaaa ggtcaagcaa cagatgtaaa aatagctgta gataatatat tgaaaataaa
    35941 agaacgatta gataaaattc tttctgaaaa tacaggtaaa tcaatagaag atattaggag
    36001 agatacagat agagataatt ttatgactgc actagaggct aaagagtacg ggctgattga
    36061 ttatataatg aataggaatg aataaagtat caaagtatca tattaaaatg tctgttttac
    36121 aaatatatat ataatattaa ataagagtgt ctcaaaatga actttttagt ttatgggaca
    36181 ctcttttttg tatgaaatta aatatggttt tatcatttca acaattatta ccaaatttca
    36241 agtttaataa attctactat aaaaaataca taaaagataa ggttctttag taactttctg
    36301 tcgtcaaaag tttttataaa tttaataaca aaacaagaga aaataccata tattacttat
    36361 gtctgcaata tatagaagac aaaggagttg tacattatga ttacaataag tttgtgcatg
    36421 attgttaaga atgaggaaga agttataggc agatgcttag aatgtgtaaa ggatatagta
    36481 gacgaaatta taattgtaga tacaggttct acagattcca caaaagaaat agtctcaaaa
    36541 tatacagaca tggtgtacga tttcgaatgg atagatgatt tctctgctgc aagaaatttt
    36601 tcattttcta aagcttctaa agactatact atgtggcttg atgcagatga cataatttta
    36661 gaagctgata gagaaaaatt attaaagatt aaagaaagct tggatacttc cattgatatt
    36721 gttatggcaa aatacaatgt atcctttgat gaaaatggta acccaactct ctcgtattat
    36781 agagaacgat tgtttaaaag aagttctaat cataaatggg ttgaccctat tcatgaagta
    36841 gtacctcttt ttggagaggt attttattct gacattgcaa tatctcataa aaagcttcat
    36901 agacaggacc ctctaagaaa tcttagaatt tttgaaaaaa tgatttcaga aggaaaaaca
    36961 ttggagccaa gacaccaatt ctattattct agagagttgt actataatgc taggtataaa
    37021 gaagcaatag aaggttttac taagttttta gactcatcaa gaggatggat tgaggattgt
    37081 atcagtgctt gcagagattt agccacatgt tattacttaa ttaatgatga aaaaagtgcc
    37141 ttatattcat tgtttagaag tttcgaattt gatgagccaa gagcagaaat ttgttgtgac
    37201 ataggaaagc atatgtttga tagacaaaaa tataaagaag ccattttttg gtataaggta
    37261 gcattaacaa gagataagaa tgatactaat ggaggtttta agtcaaatga ttgttatgga
    37321 tatataccat ctatacaatt aagtgtctgt tatgacaggt taggagaatc agataaagct
    37381 atttattatc atgaaaaaac aaaagaaatc aaacctaacg atagtgctgt tttgcacaat
    37441 gaaaattatt ttagtaaatt taaaaatagt tgataggtca caatcatact aaatttgaca
    37501 tattatatat agagcttaaa agctcaaata tatcaggagg tgcttttaat aatgagtaga
    37561 aataaatatt ttggaccatt tgatgataat gattacaaca atggctatga taaatatgat
    37621 gattgtaaca atggtcgtga tgattataat agctgtgatt gccatcattg ctgtccacca
    37681 tcatgtgtag gtccaacagg cccaatgggt ccaagaggta gaaccggccc aacaggtcca
    37741 acgggtccaa caggtccagg agtaggggca acaggcccaa caggaccaac cggtccgact
    37801 ggtccaacag gaaatacagg gaatacagga gcaacaggat taagaggtcc aacaggagca
    37861 acaggggcaa caggcccaac aggagcgaca ggagctatag ggtttggagt aacaggtcca
    37921 acaggcccaa caggagcgac aggagcaaca ggagcagatg gagtaacagg tccaacaggt
    37981 ccaacgggag caacaggagc agatggaata acaggtccaa caggagcaac aggggcaaca
    38041 ggatttggag taacaggtcc aacaggccca acaggagcaa caggagtagg agtaacagga
    38101 gcaacaggat taataggtcc aacaggagcg acaggaacac ctggagcaac aggtccaaca
    38161 ggggcaatag gagcaacagg aataggaata acaggtccaa caggagcaac aggagcaaca
    38221 ggggcagatg gagcaacagg agtaacaggc ccaacaggcc caacaggggc aacaggagca
    38281 gatggagtaa caggcccaac aggagcaaca ggagcaacag gaataggaat aacaggccca
    38341 acaggtccaa caggagcaac aggaataggg ataacagggg caacgggatt aataggtcca
    38401 acaggggcaa caggaacacc tggagcaaca ggtccaacag gagcaacagg cccaacagga
    38461 gtaggagtaa caggagcaac aggagcaaca ggggcaacag gagcagacgg agcaacagga
    38521 gtaacaggtc caacaggagc aacaggggca acaggagcaa atggattagt aggcccaaca
    38581 ggagccacag gagcagcagg aacacctgga gcaacaggtc caacaggagc aacaggccca
    38641 acaggagtag gaataacagg agcaacaggg gcaacaggag cgacaggtcc aacaggagca
    38701 gatggagcaa caggtccaac aggagcaaca ggaaatacag gagcagatgg agtagcaggt
    38761 ccaacaggag caacaggagc aacaggaaat acaggagcag atggagcaac aggtccaaca
    38821 ggagcaacag gggcaacagg agcagatgga gcaacaggtc caacaggagc
//
LOCUS       DAMJVI010000027        38125 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.028, whole genome shotgun sequence.
ACCESSION   DAMJVI010000027 DAMJVI010000000
VERSION     DAMJVI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38125
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.028"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            32..1144
                     /locus_tag="PC545_001490"
     CDS             32..1144
                     /locus_tag="PC545_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HDF2456262.1"
                     /translation="MNSRIKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLDTVRILGVLQRMGLVYFVTSLAYLLLKKLNVGSTATIITFLCIATFIIVGYYILAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPEFEPDGFLTSIVAISSGMLGCTMGCVLLKENL
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNVPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLIFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1398..3422
                     /locus_tag="PC545_001491"
     CDS             1398..3422
                     /locus_tag="PC545_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2456263.1"
                     /translation="MKKTTKLLATGMLSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGATRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNVTRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQG
                     TYNWLSSQGLEEAYYIGGSQSLSSKIIDQISKIAKNGTSKNRVSGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHNTNLSEKTAET
                     VYQIGDGMKDSVINSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIYYKVKDKNGGTTKTAASNILKRILEKFNMKNRGIKTRTLDNGKDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTAEKVKTMGTQIGIGIEDTVK"
     gene            3642..4328
                     /locus_tag="PC545_001492"
     CDS             3642..4328
                     /locus_tag="PC545_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDF2456264.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4423..5478
                     /locus_tag="PC545_001493"
     CDS             4423..5478
                     /locus_tag="PC545_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HDF2456265.1"
                     /translation="MIKKISTILSLVLLISISSTIGVFADANPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEESIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTARLDFGSRSDVNEYISLGVV
                     SAKENMPILICSDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5593..7149
                     /gene="murJ"
                     /locus_tag="PC545_001494"
     CDS             5593..7149
                     /gene="murJ"
                     /locus_tag="PC545_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2456266.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVATLLIYNNLYAFMGSGTIKEIISVGAGVLGGASVYTVLIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7195..8901
                     /locus_tag="PC545_001495"
     CDS             7195..8901
                     /locus_tag="PC545_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDF2456267.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKNLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIMKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDMANDIIAEVNKIDDYSTIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRKVLNECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKNNGTDLIIGTDPDCDRVGIVVKDSSGEYVVLNGNQVGSL
                     LVRYILESLVEENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEESNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALID
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKDGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8923..9996
                     /locus_tag="PC545_001496"
     CDS             8923..9996
                     /locus_tag="PC545_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HDF2456268.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDSNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKDEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEDISKYLGTKEEMEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10008..10730
                     /locus_tag="PC545_001497"
     CDS             10008..10730
                     /locus_tag="PC545_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2456269.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIKVLYEQGGVYMDTDMQILKDITPLLENNR
                     LVCGYEDDREYINGAIIGVEKGHPFLKDLLEYYEKEVLTSSLFTIPKIMTHLMEKNYK
                     KIDPNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTFGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10746..11903
                     /locus_tag="PC545_001498"
     CDS             10746..11903
                     /locus_tag="PC545_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HDF2456270.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRILLFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPVILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11906..12745
                     /locus_tag="PC545_001499"
     CDS             11906..12745
                     /locus_tag="PC545_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2456271.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDSNKVDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGNDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFAKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12760..13935
                     /locus_tag="PC545_001500"
     CDS             12760..13935
                     /locus_tag="PC545_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDF2456272.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHKFNGRDIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            13964..14713
                     /locus_tag="PC545_001501"
     CDS             13964..14713
                     /locus_tag="PC545_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2456273.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14735..15643
                     /locus_tag="PC545_001502"
     CDS             14735..15643
                     /locus_tag="PC545_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2456274.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALRGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15691..16443
                     /locus_tag="PC545_001503"
     CDS             15691..16443
                     /locus_tag="PC545_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDF2456275.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENVGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16421..17764
                     /locus_tag="PC545_001504"
     CDS             16421..17764
                     /locus_tag="PC545_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HDF2456276.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKVVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWAREILEEIYKPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     DAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKIKDMEELGIEYYSIGR"
     gene            17770..18861
                     /locus_tag="PC545_001505"
     CDS             17770..18861
                     /locus_tag="PC545_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2456277.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSIKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLAKELGIENDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLNMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18945..20858
                     /locus_tag="PC545_001506"
     CDS             18945..20858
                     /locus_tag="PC545_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDF2456278.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNAFTTNDIIISVCIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFVATLAYLI
                     PIYIINKLLGFDYPIMFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKVSNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSDIKDVVEKHN
                     VDEIIFSIANIEKRRKKEIIDVCKNTNCKIKTIPGIYEIIDGKVDIKQIREVEIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21057..21764
                     /locus_tag="PC545_001507"
     CDS             21057..21764
                     /locus_tag="PC545_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2456279.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAEPTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKSVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNAFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGDSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22066..24177
                     /locus_tag="PC545_001508"
     CDS             22066..24177
                     /locus_tag="PC545_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HDF2456280.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANHPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGKGQN
                     KDDWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVAWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSTSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWATNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNNSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKTNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTTL
                     VYEAGDGINQNTLNTFLNLVK"
     gene            complement(24375..25340)
                     /locus_tag="PC545_001509"
     CDS             complement(24375..25340)
                     /locus_tag="PC545_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2456281.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTVEK
                     GEIRELNKFIPETYKWSKSDDKGSIQYIRNSGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25625..26581)
                     /locus_tag="PC545_001510"
     CDS             complement(25625..26581)
                     /locus_tag="PC545_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDF2456282.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GHGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPNLVNTILPYVKTNMNPNEIISLGKELLSIGNLNLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26949..27737
                     /locus_tag="PC545_001511"
     CDS             26949..27737
                     /locus_tag="PC545_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2456283.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEDIKDDMMFISENGTLVVYKGKEILSNPLNKELVNEIIETTRSIKGKKI
                     VMSGKKYAYIESKDEAFIQEVSTYYAKFKIVEDLTKVEGDILKIAVFDFKGAEHNNNI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEHLKQIARFRAKRNTENGVVDKIKEVIKIG"
     gene            complement(27860..28330)
                     /locus_tag="PC545_001512"
     CDS             complement(27860..28330)
                     /locus_tag="PC545_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HDF2456284.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEDIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENATQIENKILNIIKK"
     gene            28469..29113
                     /gene="uppS"
                     /locus_tag="PC545_001513"
     CDS             28469..29113
                     /gene="uppS"
                     /locus_tag="PC545_001513"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDF2456285.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29156..30061
                     /locus_tag="PC545_001514"
     CDS             29156..30061
                     /locus_tag="PC545_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2456286.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKATQGVVALVKSMDKSNKTSQQQNVNSE
                     QFDLGNEEENKKKKYTVFIDPGHGGNDKGTESKTSNRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSEEQKIIN
                     EKYQDQLAEGIVQGVLSYLDSKGNK"
     gene            30376..31923
                     /locus_tag="PC545_001515"
     CDS             30376..31923
                     /locus_tag="PC545_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2456287.1"
                     /translation="MNKNSMTRIYSLNKIIFIIIGILGIVLLTRHIEVDIKVLILILL
                     TLFSINVFISYLKIKLYEDKINLGLINNSDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKNYFGKEAIYGKLKSDVFVLIIKLEN
                     REQKIPYLVNLIKNKIVILSQIDSFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     LKSKGLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMVGPNKFIPIAEKNGLINNIGRWLIQEVFITINKWISEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDIIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMHQVKVLSELGCNFIQGYVFFKPLDEMNYKKLLTDGSYINLKDTLLDK
                     CMTVEEE"
     gene            31926..32324
                     /locus_tag="PC545_001516"
     CDS             31926..32324
                     /locus_tag="PC545_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2456288.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDKPIDAKMILKSEDIPILTLYRL
                     LRKLCANNILTFNKEDGKTYMLNVSLDTISLRTIVEVIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            32364..34097
                     /locus_tag="PC545_001517"
     CDS             32364..34097
                     /locus_tag="PC545_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDF2456289.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     RHILNYCIYGADIDEKAISILKDSLTNKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYID
                     VFKIKNEDICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
                     KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYIVDKSEY
                     RLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKWYELQWGREKLFFERKK
                     IMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEYLDKFSYEYLVGILNSSVYDKYFKI
                     TAKKMSKNIYDYYPNKVMKIRIFRDNNYEEIENLSKQIISILLNKSIDKGKVEKLQIK
                     MDNLIMDSLGI"
     gene            34329..35540
                     /locus_tag="PC545_001518"
     CDS             34329..35540
                     /locus_tag="PC545_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2456290.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDASLRTVMESVIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRNLASNFEVRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            35733..35993
                     /locus_tag="PC545_001519"
     CDS             35733..35993
                     /locus_tag="PC545_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDF2456291.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            36347..38059
                     /gene="ptsP"
                     /locus_tag="PC545_001520"
     CDS             36347..38059
                     /gene="ptsP"
                     /locus_tag="PC545_001520"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDF2456292.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLED
                     AVAVSKEELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTESVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDEETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEIQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLSMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADDVLNMGTAEEIKSYIEK
                     TFNI"
BASE COUNT        15255 a         3989 c         6746 g        12135 t
ORIGIN      
        1 aatttcaaat aatgctgtag attctaaact tatgaatagt aggattaaat caatagatat
       61 aataagaggg ctatcaatag ccctcatgat agtctgcaat aatcctggga cttggatgag
      121 aatgtatcct caattaagac atgctgtatg gcatggtgta acacttgcag attttgcatt
      181 tccttttttt gttatatctt taggtgttac aattccaata tcaataaata gtaaactaaa
      241 aaataataaa tccacactga gtataatact tagtatattc aagagaagta tcttattgat
      301 attatttgga tttttcttaa attacctagg aaatcctgat ttagatactg taagaatact
      361 tggagtactt caacgtatgg gattagtcta ttttgtgact agtttagcat atttactgct
      421 aaaaaaatta aatgttggaa gtactgctac aataattact tttctatgta tagctacatt
      481 tataatagtt gggtactaca tcttagcaaa accttatgga tttgagctag aaggttctct
      541 tgcacagtta gtggatttac atttcttcaa aggacatttg tacaagccgg aatttgaacc
      601 agatggtttt ttaactagta tagtagcaat ttcttcaggt atgttgggat gcactatggg
      661 atgtgtactt ttaaaagaaa acctaggaga atataaaaaa ttctttaaaa tactggttat
      721 gagcataatc ctgctaatag gtgcttttat ctttaatcag tattttccat ttaataaaag
      781 attatggtct agctcatttg tacttttaat ggctggcagt tatggtatat tactgtcaat
      841 attttatttt atatgcgaca tcaaaaataa aagtaaaata tttaccccaa taatagcact
      901 tggaagtagc cccattttta catatatgtg ccttgagata ttgagtcatg ttttttggaa
      961 tgttccaaaa cttacgaaca aagtcgatta tccaactacc ttggtagagt ggactacata
     1021 tgaacttata acaccttggg caggtacaac ttgggattct cttatatttt ctttattgta
     1081 tgtcctattc tgggttatag taatgtctat aatgtacaag aaaaaaatat ttattaaaat
     1141 ttagtgcaat atttttagat gataaagtct ttgatttgtt caaatataca gaaactatca
     1201 aatataatca aaatttctcc tcgataaaat ttaaaaacca tgccataaaa gattacaaaa
     1261 agtttacaat ttgtaatttt ttgtgctata atgaaaaaag atttacataa tattgaattt
     1321 atgtaatata aaacaagctt aagatgtatt aaaaaagatt taaagattta aagaaccaaa
     1381 aaattagggg ggatatgatg aaaaaaacaa caaaattatt ggcaacagga atgttgtctg
     1441 tagcaatggt ggcaccaaat gtagctttag cggctgaaaa tactactgca aatacagaat
     1501 ctaattcaga tattaatata aatttacaaa gaaaaagtgt agtgttagga agtaaatcta
     1561 atgcaagtgt aaaatttaaa gaaaaattaa atgctgattc aataacatta aattttatgt
     1621 gttatgatat gccacttgaa gctactttaa attacaatga aaagacagat tcatatgaag
     1681 gtgtaataaa ctataacaaa gacccagaat acttaaatgt atgggaactt caaagcataa
     1741 aaataaatgg taaagatgaa cagaaagttt taaacaaaga agacttagag tctatgggat
     1801 taaatcttaa ggattatgat gttacacaag agtttataat aagtgatgca aattcaacaa
     1861 aagcagtaaa tgaatatatg agaaaaactt ctgcaccagt aaaaaaactt gctggagcta
     1921 ctagatttga aactgctgtt gagataagta aacaaggttg gaaagatggg tctagtaagg
     1981 ttgtaatagt taatggagaa ttagcagcag atggaattac agctactcca cttgcatcta
     2041 catatgacgc accaatatta cttgcaaata aagatgatat accagaaagt acaaaagcag
     2101 agttaaaacg tctaaatcca agtgatgtaa taattatagg tgatgatggt tcagtttcac
     2161 aaaaggcagt gtctcaaatt aaatctgctg taaatgtaaa tgtgactcgt attggtggag
     2221 ttgatagaca tgaaacatct ttacttatag caaaagaaat agataagtat catgatgtta
     2281 ataaaatata catagcaaat ggatatgctg gtgaatatga tgctcttaat atttcatcaa
     2341 aagctggtga agaccaacaa cctataatat tagctaataa agattctgta ccacaaggta
     2401 catataattg gttatcttca caaggtttag aagaagctta ttacataggt ggtagtcaaa
     2461 gtttatcatc aaaaattata gaccaaatat ctaaaatagc aaaaaatggt acttcaaaaa
     2521 atagagtttc tggagcagat agacatgaaa caaatgcaaa tgtaattaag actttctatc
     2581 cagataaaga gttgagtgca atgttagttg caaaatctga cataatagtt gattcaataa
     2641 cagcaggacc tttagcagct aaattaaaag ctccaatact tataactcca aaaacttatg
     2701 tttctgcata ccataataca aatttatcag aaaaaacagc agaaacagta tatcaaattg
     2761 gtgatggaat gaaagatagt gttataaatt ctatagcatc tagtttatca aaacacaatg
     2821 caccaacaga accagataat tcaggttcag cagcaggaaa aactgtagtg atagacccag
     2881 gtcatggggg ttctgattca ggggcaacaa gtggattaaa tggtggagca caagaaaaga
     2941 aatatactct taatacagca ttagcaacta ctgaatattt acgttcaaaa ggtataaatg
     3001 ttgttatgac tagagataca gataaaacaa tggctttagg agaaagaaca gcattatcaa
     3061 atactataaa gccagattta tttacaagta tacattacaa tgcgtctaat ggagctggta
     3121 atggtgtgga aatttattac aaagtaaaag ataaaaatgg tggaacaact aagactgcag
     3181 catcaaatat cttaaaaaga atactagaaa aatttaacat gaaaaataga ggaatcaaga
     3241 caagaacact tgataatgga aaagattacc tatatgtgtt aagaaataac aattatccag
     3301 caatacttgt tgaatgtgca tttattgaca ataagagcga tatggataag ttaaatactg
     3361 cagaaaaagt aaaaacaatg ggaactcaaa ttggaatagg aatagaagat acagtaaaat
     3421 aaaatagaaa atattaaagc aaaacatatc atctcctgtt ataatgtaaa atataacaag
     3481 agatgatata tttttgtaaa gaatcaaatt gtttacaatg atgtttcttg tattgataat
     3541 ctgctcatta ttgagtataa tatagaaata agtcaaaaat aagatagaaa taagttgaaa
     3601 ttaagaatta aaaaaatttt tattccaata tggaggtaac tatgtctgga tatactaatg
     3661 atgagtgtga aatacctaaa attaaaagct atccaggggc agataaagaa attgcaagtg
     3721 agattgacta tagcatagta aaaggaacag ttttattcga cttgtaccaa agaattatgg
     3781 atttagtttt atctataata ggacttgtga taggtttacc tttaatagca atatttggaa
     3841 ttcttattaa aatagaggac aaaggaccaa taacttataa gcaagaaaga ctaggaaaat
     3901 gcgggagaag attctatata tataaattga gatctatgag aacagacgca gaaaaatttg
     3961 gcgcacaatg ggctgaaaaa gatgatccta gaataactaa ggttggaaag tttattagga
     4021 aaactagaat tgatgagatt ccacagctgt ttaatatatt aaagggggac atgggcttaa
     4081 taggtcctag accagagaga ccgaacttta ctgttcaatt taatgaggaa atacctggat
     4141 ttatcaacag gctcgctata aaacctgggc ttacaggttg ggcacaagta aatggtggat
     4201 acgaaatcac tcctgaagaa aaacttaaag aagatattta ctatataaaa aataggagta
     4261 tattacttga ctttaagata ctctttaaaa ctgttaaagt agtattgaca ggagacggag
     4321 ctagataatt ttaaaatatt agattactaa tcaatgaaaa agctctttaa aaagagcttt
     4381 tttattgtca aaaaatacaa aaaagaaaag gaatgagaac ttatgattaa aaaaatttct
     4441 actatattat cgttagtttt gttaatctct atttctagta cgataggagt atttgcagat
     4501 gcaaatccca aaagagaatt gatagaaggg agtataccag agatttcgac agaattaaat
     4561 aaaagagcat ttaaggattc taaagaagta attcttgtaa atgaagagtc aattgtagat
     4621 agtataagtg caacaccttt agcatatgca aaaaatgctc caatagttgt aactaagagt
     4681 aaaaatcttg gtagagtaac aagaaattat ctaaaagaac taggtccaga gaaggttacg
     4741 atagtaggtg gtttaaaggc agtatctaaa gatgctgaac gtaatataga aaaaatggga
     4801 atgaaagtag aaagaataag agggaaagac agatatgata catctttaaa gatagctaga
     4861 gaaatgtata gaacagtagg atttgatgaa gcatttttac ttagctcaac aacaggatta
     4921 gagaacgcca tatctgtata ttcttatgcc gctaaaagtg gaatgccaat aatatgggct
     4981 aaagatgaag ggtttgagga acaaattgat ttcttaaaag gcaaaaatct taaaaaaata
     5041 tatgcattag gtgattcaaa agagtttatt gcagagattg attctaactt aaaaaatata
     5101 gaaggaataa agcaaataaa taagtcaagt acaaatgttg atttaataaa gaagttttat
     5161 gatgaaaaag atataaagaa aatctatact gctagattag actttggtag tagatctgat
     5221 gtaaacgaat atatttcttt gggagtagta tcagcaaaag aaaatatgcc aatactgatt
     5281 tgtagtgata acttaagtcg tgcacaagat aaattcttga aagatagcaa tataaatgat
     5341 gtagttgaag ttgggtatac agtaggtgat tatagtctat ttaagagcat atttaatcta
     5401 acattcttat cttgtatagt attaatacta ttattactat tgattacatt tagggcactt
     5461 agatatgagt cgaaatagtt tttgatttat aaggaagctt ttcataggtt aaatttactt
     5521 aattagaata atgtttataa gcagttttaa aagttctact tttagagttt aaattagata
     5581 aggagaaaga agatgtcaaa aacagctaaa gcagcattgt ggattatggc agccactatg
     5641 ttttcaaaag tattaggttt ccttagagaa cttgtacttg caaatttcta tgggacaggt
     5701 atgtatgcag acgtatttgt attaacatta aatatacctg gtttaatcat agcagtcata
     5761 ggctctgcag ttgctacaac ttatatacct atgtactttg agacaaagaa aagactcgga
     5821 gatgaaggtg ccttaaaatt tactaataat gttttaaata tatgctatat aatggctata
     5881 gtaatagcta ttattggtct tttatttaca gagcaatttg ttacagtatt tgcagcagga
     5941 tttagaaacg accctgctaa gttccaagca gcaatattat ttactaaaat aatgatttca
     6001 ggagttttat tcctgagtgg aagtaagata tttagttcgt ttttacaggt gaatgacagt
     6061 tttgtaattc ctgggcttat aggaatccca tataatatta taataatagc agcaatagca
     6121 ctaagtgcag gtaaaaatgt atggatactg ccagcaggag cattattggc tatggcgagt
     6181 cagctattgt ttcaactgcc atttgcgttt aagaagtcct acaagtataa gccatatata
     6241 aatttaaagg atgagtctat aaaagagtta gtcaacctag tattgcctat gttggtagga
     6301 gtagctgttg gacagttaaa tatatttgtt gaccgattat tggcaaccac tttgggtgat
     6361 ggaaagttgt ctgctcttaa ctatgcaaat aggctaaatg agtttgttat ggcattattt
     6421 gttacatcta taataacagt tatatatcct aaactagcaa aaatgtctgg taaagataat
     6481 aaagaaggat ttataagcac tattgtaaaa tcttcaaatt gtattatatt ggtggtgctt
     6541 cctatatcta taggagctat tatattagca gagcctcttg ttagaatatt attccaaagg
     6601 ggtaaatttg atgcgttatc aacagacctt acatctatag cgcttagatt atattcacta
     6661 ggtttattgg cttgtggagt aagagatgtc ttatataggg cattttactc tctttcagat
     6721 acaaaaacac caatgataaa tggaagtata gctttaataa taaatatagt tcttaattta
     6781 atattgataa gaccattggg tcatgcaggt attgctatat caactagtac atcaaacata
     6841 attacagtta tattattatt tatatctctt aaaaagaaaa atggatattt tggaggggat
     6901 aaaattataa agactggtct aaaaagtttg gtagcatctg gtgtgatggc agtagctact
     6961 ttattgatat ataataatct atatgcattt atgggttcag gaacaattaa agaaattata
     7021 tcagttggag ctggagtatt aggtggagct tctgtatata ctgtattgat agttttattt
     7081 aaggtagaag agatggattt agcttttgaa tttttaagaa aaggaaaaca aaaactactt
     7141 agaagataga taattacctt gaaattttaa attagagtca gaaaggatga aaatatggat
     7201 tataagaata attatgagat gtggttaaat tctccttatt ttgatgaaca aacgaaaaat
     7261 gagctattaa gtataaaaga tgatgaaaaa gaaatacaag atagatttta taaaaattta
     7321 gagtttggaa caggtggttt aagaggtata ataggagcgg gaactaatag aatcaatatt
     7381 tatactgtta gacgtgcgac actgggcgtt ttaaattata tcatgaaaac tcaaggagaa
     7441 gaaggtaagc aaaagggaat agttatagca catgatagta gatacatgtc tagggagttt
     7501 tgtatagaag tagcaaagac tttaagtgct tatggtgtaa aggcttatat ttttgaagaa
     7561 ttaaagccta caccagaact ttcttttgct gttagatatt taaagtgtgc tatgggaata
     7621 gtaataacag caagtcataa tcctaaagaa tacaatggat ataaagtata tgactcagat
     7681 ggtggtcaaa tctgtataga tatggcaaat gatataatag cagaagttaa taagattgat
     7741 gattacagta ctattaaaag tatagatttt gaagaagctt tatctaaaaa tttaataact
     7801 atattagata atgaagtaga tgatgagttt ataaaagctg ttaagaaaca agttttaaga
     7861 caaaatataa tagatgaata cgggaaaaaa ttaaaaataa tatatacacc tattcatggt
     7921 acaggaaata agccagtaag aaaagtctta aatgaatgtg gttttgaaaa tgtaatggta
     7981 gttaaggagc aagaattgcc agattctaat ttttcaacag taaagtatcc taatcctgaa
     8041 gaaaagtcag tgtttaatat agctattgaa atggcaaaga ataatggcac tgatttgata
     8101 ataggaacag accctgattg tgatagagta ggtatcgttg taaaagattc aagtggtgaa
     8161 tatgtagtct taaatggaaa tcaagtcggt tcacttttag ttagatatat attagagagt
     8221 ttagttgaag aaaataaact tcctaaaaac aatcctacaa taataaaaac tatagttaca
     8281 tcagaactag gtgcaaaaat agcaaaggct tataatgtag attgtttaaa tactttaaca
     8341 ggatttaaat ttataggaga aaaaataaaa gcgtttgaag aaagcaatga tagaagtttt
     8401 ataatgggat atgaggaaag ctatggttac ttaattggaa ctcatgctag agataaggat
     8461 ggtgttgtat cttcacttat gatatgtgaa atggcagcat actattcttc aaaaggtatg
     8521 aacttgtatg aagctttgat agatacatat aataaatttg gatactacaa agaggactta
     8581 aagtcagtaa ctttaaaagg catagatgga ataaaaaaaa taaaagagat gatgctttac
     8641 tttagaagtg ttaaaataga taatgtggct gatgtaaaag tagataaaat tttagactat
     8701 aaagatggtg ttgatgattt acctaagtca gatgtactta aatttttact tgaagatggc
     8761 tcttggatag ctataagacc ttcaggtaca gaacctaaaa ttaaatttta ctttggagca
     8821 aattcagaca atcaagaaga tgtagaattt aagttgaaca atttaatatc ttatatatta
     8881 aatgtagtgg attctatata aaaagctaag gggagataaa caatgaaagt atacaatgtc
     8941 ataatggctg gaggaggagg aactcgtttc tggccactta gtagacaaga agtacctaaa
     9001 cagcttataa atttaagtgg agaagatgct ttaataaacg aaactataaa cagaatagat
     9061 tctctagcaa aaaaagatga tttatttata gtaaccaatg agaagcaatt ggaagcttta
     9121 aaggatatag taaaagataa atgtttggat agtaatatat tgccagagcc atgtgccaga
     9181 aatacagcag cagctatagg ctttgcagca tttaacataa tgaaaaaata tggtgatggt
     9241 gttatgtgtg tatatccagc tgaccattat ataaaagatg aaaaagaatt taaatctatt
     9301 ttggaaaaag ctatctatat agcagaaaat aatgataaac tagtaactat tgggataact
     9361 cctacatttc catcaacagg atatggatac ataaatttta atagagaaaa tactatagaa
     9421 gatgttgcat atgaggtagt ggagtttgta gaaaaaccaa actatgaaat tgctaaagaa
     9481 tatgtaaact ctaaaaagta tgtatggaat agtggaatgt tcgtatggaa ggtatctaaa
     9541 atactagaag attttaagag atacttgcca aaagtatatg aaaaattaga agatataagt
     9601 aaatacttag gaacaaaaga agaaatggaa aaaataaaag aaatataccc aactatacaa
     9661 tcaatctcta tagattatgg tattatggaa agaagtaatg atgttatagt agttcctggt
     9721 gattttggat ggaatgatgt tggttcttgg gattctctag gtgcaatata tccaactgat
     9781 gatgaaggta atatcaaaag aggtgaaaat attactatag atactaaaaa ttcaattata
     9841 tattcagatg acaaattaat atcaacaata ggtattagtg atttgatagt agtatctaca
     9901 aatgatgcag ttatggtttg tagaaaagat aaagctcaag atgtcaaaaa aatagtagag
     9961 cagttaaaag aagaagatag acaagaatat atgtaggagg tgttcatttg attcctaaaa
    10021 aaatacatta tgtatggttt ggtggtccaa aaggaaatat agaaaatata tgtataaatt
    10081 cttggaaaga aaagcttcca gaatatgaga tagtagagtg gaatgaaaaa aactttgata
    10141 tagagaaaga aataaaagga aataagtttt tggaagaatg ttataaaaga aaactttggg
    10201 cattcatatc tgattataca agaataaaag tactctatga acaaggtgga gtttatatgg
    10261 atacagatat gcaaatatta aaggatataa ctcctctttt agaaaataat aggctagtct
    10321 gtgggtatga agatgataga gagtatataa atggtgctat aataggtgta gaaaaaggac
    10381 atcctttttt aaaagactta ttggagtatt atgaaaaaga agtacttact tcttcattgt
    10441 ttacaatacc aaagataatg actcatctta tggaaaagaa ttataagaag attgatccaa
    10501 ataattatga agaaggaata cgtgtttacg acaaagaata tttttatcct tttggattta
    10561 aagaagactt tacaccagaa tgtataacag aaaacacttt tgggatacat tggtggggaa
    10621 aaagttgggc taaaaagaga aattatttct tagaaagcaa acacttaaca ggtgtaaata
    10681 agatatggaa gtgttgtaaa atatttgcaa gtaatacttt acgaagctaa aagggagaaa
    10741 gtaaaatgaa gactttaaat gatgatagaa atattatgat tagaaaatat ctttatatat
    10801 ttgttttgtt tatcatatta cttagaaagt atatgatatc atttattgac ccaaatatgg
    10861 atatagggat gattaaatca gcgttatttt attcttcaat aggaatacta atgctacttt
    10921 ttttatttga taagagaaaa agtataacag aaatggtact agttggagtt tgtgtcttac
    10981 tttatttatt aaatagggaa ggagctatat tattaatcgt tttattagct gtatcagcta
    11041 agcaaataga tgataaattt atagttaaaa attatttaat aatatcggca tgttttttaa
    11101 tggtagctat attattattt aatttatttc cttcattaat atttaatcag gaagtccact
    11161 atagatatat agaaaaaata gatatgcttg ttacaagaat ggattttgga cttggtaatc
    11221 caaatagtgt attttatcat atggttacta tatatgcagc atatatattc ttaagatata
    11281 aagattataa taaatgggat agaatacttt tatttggttc tgcatttttt gtttatcaaa
    11341 ccacatatag tagaactgga tttttcacta tattagcagg attaatattt gtagaaataa
    11401 taagatggat agatataaaa aaaataaaag gactaccaat gctcctaaag acattaccgg
    11461 taatacttac tttatttagt gtaattattg gtacagtatt tgataaaagc actttattaa
    11521 atagattatt agcaagtaga cctaagttct ggaatgtata cttggctgaa aaggggaact
    11581 ttctaaaacc ttttggaaat tcatatagtc ctgctataaa agcaactaat ccattagata
    11641 gttcttatgt atatataatt agtgtactag gagtagttgc ttgtatatta tttatgtatt
    11701 tgatgtataa aggaatagaa agctttatag aaaaagataa aaaagcatat ttagtagctt
    11761 tatttatatt tttactatac tcttttgcag aaaatttatt attagaagct tcatttagtt
    11821 ttgcagtagt attactaata aaagaagtga tattaaatga taaaagggaa atagatttgt
    11881 ggaagatgaa aagtaggagg tagtaatgtt aatatcatta ataatgccaa cattaaatag
    11941 atatgatgat atatacttac ttatggacag cttagaaaat caaacttaca aaaactttga
    12001 acttatagta gtagaccaaa atgataatag caaagttaaa gaaatagttg ataaatacat
    12061 tgataagtta gatataaagt atataaaaag ctctaaaaaa ggattgagtt ataacagaaa
    12121 tgtaggcata gacaatgctg tagggcaaat tataggtttc cctgatgatg attgtgtcta
    12181 cgaaaatgat acattagaaa aagttataaa tttctttaat aaaaataaag attataaaat
    12241 atacagttgc aaaactatgg attcaaataa agttgatgca tttaagaaaa tgtatgatgg
    12301 aacttgtgat atcacaagtt ccaacgtttt ggatactata acatccataa ctttttttat
    12361 tgattttgaa ggtaaagact atacaagatt tgatgaaaaa ttgggtgttg gtggagagtt
    12421 tggtgctggt gaagagatag attatgtttt aaacttattg agtttaggtt ttaaaggaaa
    12481 atactttgga aatgatataa tttaccatcc tgcaaaaaaa cattctaaat ctaaagaaaa
    12541 gtatcaaaaa gattataact atggaagagg ctttggagcg ctttgcaaaa aggaaatagt
    12601 ctatagaaaa aattataagt ttgcaaaagt tatggtatca aaacttgtaa gaaatatagg
    12661 aggtcttata ttgagttcca atagagatta tcatagtgcc actataaaag gaagaattaa
    12721 tggatttaga cagtacaagt tataatgagg atggtgttta tggacaaggt taaattttta
    12781 ataaatatgc ttttggcttt ttttatatat ccatttaata agcataagtt taatggtaga
    12841 gatatctggc ttataggtgg acattcaggg gatatataca atgataattc aaagtttttt
    12901 tatgagtata tgctaaaaga acataatgat gtagaaactt attgggtagt aaataaagac
    12961 agtaaagtat ttgataaaat tccaggcaaa aaattaataa gaggaagtgt agaaaactat
    13021 ctgtattatt acaattcaaa agctatagta ttttcacatg caccatcagc agatatagca
    13081 ccatacaatt ttgctgtacc tgttcttaat tattttcata aaaaaacgat aaaagtgttt
    13141 ttaaatcatg ggactataag ttttaaaaaa agaaaaccta tgaacaaaaa gttcaaaaat
    13201 attatagata acctatataa aagctataat atagttacag caagttctga atttgaaaga
    13261 aatgttatgg taaatgattg gggaatgtta gatgactcag tttatataat tggaaatgca
    13321 agatatgaca atttaccaac aaatgaagtt gcacaaactc gagatatatt atatactcct
    13381 acatggagag attggataaa atttagttca ggaaaattta cggatacaga ttattttaaa
    13441 aatataatga attttttaaa tgatgacaaa ttaaataaaa tattagatga gaaagatata
    13501 aatgtaaaaa tttatatgca tcatcttatg catgagttta tagatgatat aaaagaaaat
    13561 ataacaggaa aacgaattgt gtttttagat aaaggggtaa ctctagcaaa tgaaatcaga
    13621 aaatcagctg caaatataac tgattattca agtgtagcca tagacttttt gtatatgaat
    13681 agacctatct tgttttacca attcgatttg gatgaatata tggaaaaagt tgattcatat
    13741 atagatttaa aaagtgaaat gtttgggtct ttggcttata ataatgatga agccgtgaat
    13801 aaacttattg atattattga aaataatttt gaggttatgg ataaccaaaa aaatgaaaga
    13861 aataagtttt tcagatataa tgataataag aactgcaaga ggatatatga ttgtgtatta
    13921 agtaaaatta aataaatatt ttataaatga tgaggaaggt aatatgaaga agaatttagt
    13981 atctataatt actcccatgt ataattctga aaaatttatt gaagcaacca taaaatcagt
    14041 attaaaccaa acttatcaag aatgggaaat gttaattatt gatgattgct caacagataa
    14101 tagtcctaat atagtcaaat cttatatgca acaggatagt agaataaaat gtataaagac
    14161 tgagactaat aagggtgtct ctaatgctag aaatttagca ctaagtaagg caacaggaca
    14221 atttatagct tttttagata gtgatgacca atggaatagt agtaagttag aaaaacaagt
    14281 aaattttatg ttagaaaatg actatgtaat ttcatttact tcatatgaac tgatggatga
    14341 aaatgataaa aaattaaaca aagtaataaa agttccacct aatgtagatt atagaagatt
    14401 attaaaagga aatatcttag gatgtcttac tgttgtgatt gataagtcta aattggattt
    14461 tgaaataaga atgagtggtg taagacatga agattatgtt ttatggttat cgatattgaa
    14521 aaaagggcat attgctcatg gaataaatga agttctagca ttatatagaa aatctagcaa
    14581 ttccctaagt ggtaataaaa taaaagctgc tatgtggact tggaatatat atagaaatat
    14641 agaaaaaata cctttataca aagctatata ctactttatt aattatggaa taaacggaat
    14701 caaaaaaagt tagacagaga ggaagattta attaatgtat aaatataaat ttactgtatt
    14761 tactccgacg tataacagag ctcatttact tgaaaatttg tacaatgatt taaaagctca
    14821 aacttatgat ttcaatgatt ttgaatggtt gattgttgat gatggaagta gcgacagtac
    14881 taaagagtta gttgaaaaat ttatatcaga aaataaatta aatataagat atatatacaa
    14941 agaaaatgga ggaaaacata ctgcaataaa tctaggtgta aaaaatgcag atggagagct
    15001 atttttcatt gtagatagtg atgatggttt aattaaagat tctcttgaaa ttgctaatga
    15061 tgagtgggat tctttagaga ataaagaagg atttagtgga gttgttggac tttgtgtata
    15121 tccaagtgga gatttgatag ggacagaaat gcctgaagat aagaaaatat gtcactttgc
    15181 agacctatat tttaagtatg gcgttaaggg agataaaagc atagtatttg taactgatga
    15241 gcttataaaa tttccttttc cagaaagaaa agaagtgaga tttttaccag aaagtgtagt
    15301 atggaatgaa atgtctaaat actataaagt tatatgtgta aataaaccta tgataataag
    15361 agattatttg gatgatggtt tgactaagaa tatattaagt aaaaatgcat taagaggtag
    15421 agcattagag tttttatatt taataaatca aaatacatat ccattaagta gatatccata
    15481 tatgtggata aaaaattaca taaatttagc cagatactca ttgttgtctg atagtcatta
    15541 ttttggtgaa ttaagaaaag tatctgataa attgttgtat ttagcattat tcccattagg
    15601 atattacaaa tatattggac aaagaaaatt agtatctaag taaacttgtt ggtatagtac
    15661 tgctagttgc tatataagga gagtaaaaat atgaatgagc ctttagtatc gataattact
    15721 cctgtttata attcagaaga gtttttatca gagacgataa agtcaatcca aaatcaaact
    15781 tataaaaatt ggcagctttt attagtggat gattgttcta aggacaattc aagtgaaatt
    15841 ataaaaagtt ttcgaaaaga agatgctaga ataaaatata taaagcttga aaaaaattct
    15901 ggagctgcag tatctagaaa tgttggtatt aagaatgcgg aaggtcgatt tattgcattt
    15961 gtagatagtg atgatttatg ggatagtaga aaattagaga ttcagataga atatatgtta
    16021 aaagaaaatg taggtttttc ttttacatct tatagatata tgagacaaga tgggtcaaaa
    16081 acgaataagg ttgcaagagc tcctaagaaa attgactatg aaggattact tagaaatact
    16141 ataataggat gttctacagt ggtcattgat aaggagattg taggagagtt cagcatgcct
    16201 ttggtaagaa gaggacaaga tactgcaacc tggttacagt tacttaaaaa ggaaaaatat
    16261 gcatatggga ttcaagaaga tttagtgaat tacagattag ttggaaattc tatatcaagt
    16321 aacaagataa aagcattaaa acgaacttgg aatacttata gaaatgttga aaatttaagt
    16381 ttaccaaaaa gtctatatgt attttgtttc tatgttttta atgccattaa aaaaagagta
    16441 taagttttat atacttggag gtaaaaaaat gaaaatagct atagctggga cgggatatgt
    16501 aggtttggtt acaggtgttt gtctagcaga ggtaggcaat gaaaatgtag tctgtgttga
    16561 tgtagatgaa caaaaagtta aatcaatgaa acttggaata gcacctatat atgaagaagg
    16621 cctagaagaa cttatggtta aaaattacaa cagaggaaga atagattaca ctacagacta
    16681 taaaaaagct tatgaagatg tagatgtaat attaatagct gtaggtacac ctgagagaag
    16741 tgatggttca gccaacctag attatataaa ggtagttgca aagcaaatag ctgaaactgt
    16801 gacaaaagat actctagtag taataaaatc tacagttcca attggtacaa atgaagagat
    16861 agaagattat ataaatagaa acctaaaaaa tgatgtaaaa gtagaacttg catctaatcc
    16921 agagttttta tcacaaggaa cagcagtaaa agatactcta catgcttcaa gaatcgttat
    16981 aggtgtagaa agtgaatggg ctagagaaat attagaggaa atctataagc cttttaatca
    17041 acctataata gttacaaata gaaatagtgc tgagatgata aaatacgcat caaataactt
    17101 cttagctctt aaaatatcat ttatgaatga tattgcaaat ctttgtgaaa tagcaggagc
    17161 taacatagaa gatgtagctt taggaatgag ttatgatgat agaataggaa gtaagttttt
    17221 aaaggcggga ataggatatg gtggttcttg ctttccaaaa gacacaaaag ctcttcactg
    17281 gttatctgaa cataatggtt atgaattaaa aacagtaaaa gctgcaatag aagttaatga
    17341 aagtcaaaaa cttaaattag ttagaaaggc atctaagtta gtagagagct ttcaagatat
    17401 gaaggttgct gttttaggtt tgacttttaa gccagacact gatgatataa gagaagcacc
    17461 atctatacca aatataaaat atttgctcga aagaggggca agagttactg tatacgaccc
    17521 aattggtgta aataatacta agaaaatatt tggtgactct atactttatg ctgattctgt
    17581 agatgatgcg atagaagatg cagagatatg ttttataatg acagaatgga aagatgtatt
    17641 aaattatgat atacataagt ataaggaaaa gatgaaaaat cctttagtcc ttgatggaag
    17701 aaattgttat aaaattaaag atatggaaga gttaggaatt gagtattatt cgataggaag
    17761 gtaaatatta tgaagactgt ttgttattta gctgatgggt caaatcctca tactttaaaa
    17821 tggtgtagtt tttttaaaaa taaaggatat gacattcatg taatctcttt aaatggaggt
    17881 cacatggaag gaataaaaat ttataatttt ggttctaatg tagaagaact aaaaaatgaa
    17941 aatatttcta aaaaaacggg atacttaggt tcaataaagc agataaagaa gctagtaaat
    18001 gaaataaagc cagatatatt gcatgcacag tatgcaagta gttatggatt tataggaagt
    18061 ctacttgggt atcatccata tattatttct gtttggggta ctgatattta tgatttccct
    18121 aataatggtt ttatacaaaa acaaattata aagcataact ttagaaaagc agactatatc
    18181 ttttcaaata gtagagatat ggctaaagag gctaataaat atacagctaa acatgtagat
    18241 gtaacctttt ttggagtaga tatggatagg tttaagccta tggaagtaga aaaagaagac
    18301 gcttttgtaa taggaattat aaagagttta gaaaaaaaat atggaataga atatttaata
    18361 caagcattta agatgcttaa agatgaatat aaagataaga aaataattct taaaataggt
    18421 ggctctggaa gtcagatgga caacctaata aatttagcta aagaattggg tatagaaaat
    18481 gatgttcagt ttttaggaag aatatctcca gaaaatgtaa gcaagacttt taattctttt
    18541 gatgtaactg tatttccttc tttaagagag ggatttggag tggcagctat agaatcagaa
    18601 gcatgtgaag tacctgttat agttacaaat gtaggaggac atccagaatc tgtatgggaa
    18661 aatgaaactg gcttaattgt agaaccaaaa caaccagaag aaataaaaaa tgctataata
    18721 aaactaatgg aaaatgatga acttagactt aatatgggta agaaagggcg tcaatttgtt
    18781 agagaaaatt atgaggttaa tttaaacttt aatgatatag aaaaaattta tgattctata
    18841 tttgataaat ataaaaaata aaatgaaaaa atacagcaaa agatagatgt tgttggctat
    18901 gcttaggtgc gtagcttttg ctgtttaaag gagggcttct agttatgcga gaaagtatag
    18961 atttagagaa agaaagcttt tttaataggc tttttaaaaa atataaattt agacatttaa
    19021 cactattaat tctagatatt tgtagcgtac tagtagcgtt tcgaatgtct ttaagcttga
    19081 caggtaattt aaatgcattt accactaatg atataataat ttcagtgtgt atatatatag
    19141 tacttcatat agtatcattt agattgttta agtgctacaa cacattatgg agatatgcag
    19201 gagaagaaga aattatatct atttttgtgg caactttagc ttatttaatt ccgatttata
    19261 taataaataa attattaggg tttgattatc ctataatgtt ttatgtatta aatacaatat
    19321 ttattataat gtttacaagt ggcgcaagaa tagcctatag agctataaga atagtcatga
    19381 acaagacata ttctagagga aaggtttcaa atatccttat aattggtgca ggagatgcag
    19441 gagaaatggt tatacaggaa ctaaaaagaa atccagaact taagaaagta gcagtggcta
    19501 taattgatga tgataaaaat aaaattggaa gaataatcca taatgtgaaa attgtaggaa
    19561 caacttcaga tataaaagat gtggtagaaa agcacaatgt agatgagata atcttttcaa
    19621 tagcaaacat agagaaacgt agaaaaaaag agattattga tgtatgtaaa aacactaact
    19681 gtaaaataaa gacaatacca ggaatttacg aaattataga tggaaaagtt gatataaaac
    19741 agattagaga agtagagata gaagacttgt tgggtagaga gcctataaaa acaaatttaa
    19801 gagagataag taattacatt gagggaaaag taatcctaat aactggagga ggaggctcaa
    19861 ttggttcaga gctttgtaga cagattgcag gatttaatcc taaagagctt attatagttg
    19921 ataattatga aaataatgct tactcaatac aacaagaatt acttagaaaa tataaaaata
    19981 aattagattt aaagacagta atagcatcaa taagagaaga aaaaagaatg gatgaaatat
    20041 ttaataaata taaaccagaa gtggtatttc atgcagcagc acataaacat gttcctctta
    20101 tggaatcgag ccctggcgaa gctataaaga ataatatatt tggaacttta aacatagctg
    20161 gcttgtcttc aaaatataga gctaagaaat ttgtccttat atctacagat aaggctgtaa
    20221 accctactaa cataatggga gctactaaaa gagctgctga aatgattata cagactatga
    20281 atgctgaaag ccagactgaa tttgttgctg ttagatttgg aaatgtgctt ggtagtaatg
    20341 gtagtgtaat tccacttttt aagaagcaaa ttgaagatgg cggaccagtg acagttactc
    20401 atccagatat tattagatat tttatgacaa taccagaagc agtaggctta gtcatacaag
    20461 ctggggctat ggctaaaggt ggagaaatat ttgtacttga tatgggagaa cctgtaagaa
    20521 tattggattt agcaaaaaac ttaattaaat tcagtggatt tgagccagat gttgatataa
    20581 aaattgaatt ttctggtctt agaccaggag aaaagttata tgaagaacta cttatgtcag
    20641 aagaaggtct gttggataca gagcataaaa agatttttat tggtagacca atagatgttg
    20701 atagagaaaa aataacaaaa tacttaaaac ttttaagaga aattactaac aatgaagagg
    20761 ttgaaaaaat agatggtatt atgagggaat tagtgccaac ttatattaaa ccagaagatg
    20821 ctaatatcaa agaaattgca acaactagag aaaagtagaa tatatttcta atttgtaacc
    20881 agggggagca aaaagaaaca aatatatact aaaaagaaac aaaagattac aaaaagatta
    20941 caaaaagatt acaaaaaagg ttgcaattta ttttgatgta agatagaatg ttaattagga
    21001 attggaaacg aaggaacaaa atgttaaatt ttcgaaagga ttaggtattt gagattatga
    21061 tgaatattaa aaataaaaaa catatattga aaaaatttat agctatggtg ttaatagcag
    21121 gagttgtcac agtagaagca ggagcaataa cggctagtgc agcagagcca actaattcac
    21181 ctatgagtgc aacagtagac cagtgtgatt ttttaaatgt aagaagtgga gctagtgcaa
    21241 atgatgcagt agtaggaaag ataaacacag gagacaaagt tgaagtttta gaattacatt
    21301 caaatggatg gattaaaata aaaagtgttg acaatgttac aggatgggta aatggagatt
    21361 atttaactat tcaaggtgga aatgtagatg caaaggtaca aaatgtttta aacttagcat
    21421 ttaagcaaca aggtaagcca tatagatggg gagcaactgg accaaatgct tttgattgtt
    21481 ctgggttcac ttcttatgtc tataaaaatg gagcaggagt taatttacca agagtatcaa
    21541 gatcacaagc aacagtagga aagaaagtat caagagcaga attaaaacca ggagatttag
    21601 ttttctttgg ttcaggtggt tcaataaatc acgttggatt gtatgtagga gattctaaat
    21661 tcatacattc accacaaaca ggagatgtag taaaagttac tagcatggct ccaggaacta
    21721 attatgcaag aagacttata actgctacac gtgttttaca ataataatag atattgattt
    21781 acataggatt gaatattaac attaaatata taattacatt taatgtttta catagagatt
    21841 taagagttta gtaatattaa aatagtgatg aacgttataa aaacagcaaa gaagataaat
    21901 ttctttgctg ttttttttat aataaattat ttcatattat aatcaaatat caaatataaa
    21961 ttttaactat attattcaat atttgacaga taatatgaat tttagatata cttaataaga
    22021 agtatattgt agaaaaaata aatttaaaaa attaaggggg atgaaatgaa aaaaataagt
    22081 atattagtat tatcattgat aatgacactt actatgtgta gtgtaagttc atttgctgat
    22141 tcatcaaatg ataaagaaat gagagctgct tggatatcta cagtttataa tttagactgg
    22201 cctaagacta agaataatga agccaagcaa aaaaaggaat acacagattt attggacaaa
    22261 ttaaaaagtg taggaataaa tacagcagtg gttcaagtaa gaccaaaatc agatgcttta
    22321 tataaatcaa atataaaccc atggtctgaa tatcttaccg gtacacaagg taaagaccct
    22381 ggatatgacc cattaccatt tttaatagaa gaagcacata aaagaggaat ggaatttcat
    22441 gcatggttta atccatatag aataactatg gcagatgaga gcatagataa attgccagca
    22501 aaccatccag caaagaaaaa tccaagctgg gtagttaaac atggaaataa gtattactac
    22561 gacccaggtt taccagaagt tagaaagtac atagtggata gtatagcaga agtagttcaa
    22621 aattacgata tagatggagt tcattttgat gattacttct atccaggagt aagtttcaat
    22681 gatactgcaa cataccaaaa atatggaaaa ggacaaaata aggatgattg gagaagagaa
    22741 aatgtaaata ctctacttag agatgtaaaa gcgtctataa agagtataaa gcctaatgtt
    22801 gtatttggtg taagcccagc aggtatatgg agaaataaga gtagtgaccc aacaggttca
    22861 gatacatcag gtaatgaaag ttatgtaggt acatatgcag atacaagagc atggataaaa
    22921 caaggattga ttgactatgt agttccacaa ctttactggc caattggtct taaagcagca
    22981 gattattcta aattagtggc atggtgggct aatgaagtaa aaggaacaaa tgtagactta
    23041 tacataggac aaggtatata taagcaaggt caatcgagtt atggtggaca aaatatagca
    23101 aaagaaatag tacaacaagt aacattaaat agaaagtata gtgaaataaa aggtagtatg
    23161 tatttctcag ctaaagatat agcaaatagt acttctatac aaaaggattt aaagagttta
    23221 tattctagta gtgaagaacc agttactcca ccatctaatg ttaaagtgga gaaattaaga
    23281 ggcgacgaaa gatatgacac agcagtagca ataagtaaaa aaggttgggc tacaaattca
    23341 gatacagtag tattagtaaa tgggtactct atagttgatg gtataacatc aacaccactt
    23401 gctacatcaa atgatgctcc tatactattg gtaaataagg ataatatacc tacatccact
    23461 aagaatgagt taaaaagact taatccaagt aaagttattt taattggagg aaataactct
    23521 attggagata aagttgagag tgagataaaa gatacattgt caaatgtatc aataaataga
    23581 gttggtggtt cagatagata tagtacatct ttaatgatag caaaagaatt agtaaaaact
    23641 aatccagtag aaaaactata tataacaagt ggtacaggtg aagcagattc tctttctata
    23701 gcttcaaaag caggagaaga aaagcaacca atagtattag tatcaaaaga taatgtaagt
    23761 gatgaagttt acaattggat atcagattta aaagtaaaag atgcttattt tataggtggt
    23821 aatctaagta tatctgattc agtaataaat aagctggata aggtcattac aaatgatgta
    23881 tctaaaaata gaatagctgg agaaaataga gaagaaacaa atggaaaagt aatacaaaaa
    23941 ttctatccaa atgctgaata ttcatcaatg tttgtaagta aaagtaatca attggtagat
    24001 gcattaacat caggtccttt agcggctaaa ttaaaatcac cagtagttat gcttggaaat
    24061 agtgtaacat ctgctcagaa aactgcttta gagcctaaga aaactactct agtatacgaa
    24121 gctggagatg gtataaatca aaacacatta aacactttct taaacttagt taagtaataa
    24181 aaaagttaat ttaaaataca gagtatttta caaatgtaga ttaaaatgaa aattatcatg
    24241 ataaattaga aaataaaaaa gaactatatg cattttgtaa tcaagtgtac acagcaactt
    24301 gaaaataatt atgcgatata gttctttttt agtaattaat cttatacttt ttaataaact
    24361 ttatgttgaa ttttttattg tttaaactct atgtcattaa atataaaatc gtgtaatatg
    24421 tctaaagtat ctgggtcaaa tctaagaacc catccagctt ttccatatat accacctgta
    24481 ctatgaattt catcatcaat aggaaattca aattgcttaa tatttaagtc acccattttt
    24541 aaaacttgag ctcctaaaga aagtattgca ttaggcttca tgttagtttt aacatatgga
    24601 agaactgcat ctaataaacc tggatattta gtaacaggta aagtttccgt tttcttgatt
    24661 atagcttgta taatctgtct ctgtctgcca tctctagcaa aggctgtatc gttgtgtctg
    24721 atacgtgcaa aagatagtgc ctgatatcca tttagagtct gctttccaga gtttctaatg
    24781 tattgtatgc tacctttatc atcactttta ctccatttat aagtttcagg aataaattta
    24841 tttagttctc gtatttctcc tttttcaaca gtaacctcaa cgccaccaat agcatcaatt
    24901 atatacataa atgattcgaa attaacacaa gcataatttt gaatatcaat attaaaattc
    24961 tcttcaattg tttgtattaa taaatcagct tgtccataag cataagcatg agtaagtttt
    25021 tgtttaccat gaccagggat gtctacatag ctatcacgag ctaatgatgt aagtttgata
    25081 tcattgtgtt tgttatcaat agtaagtatc atcatagcat ctgaccttga agaatcttca
    25141 tttggtctag catctgttcc cataagtagt atgttaatta tcccatcttc atttttatgg
    25201 tcattgttat ccaataaatc actacttatg ctcgagtcat gaatagcaga taatttataa
    25261 taaaaatatc catagacact tatagggaat attactacca agaaagccag tagtataaca
    25321 aatttcttta attttgacaa aagtacccct tctttcttct ttttcctgta tattttttaa
    25381 tagcatacac ttaattatat taaagtattg agatatattc aatacttgtt tcagttacaa
    25441 aaaaaagaca atgatataat ttataagaaa atttatcgac ctatatatat agttaaaagt
    25501 atatataata tattttttaa aagtaattta tattttaaaa taaaaacaca ccccataaat
    25561 acaaagtaat ttgcatttaa gaggtatgta tttatcatca aattattggt ttgttttgga
    25621 atttctaatc ttcaaccata atatctttaa atatgaaatc atgcattgca tctagttcat
    25681 attcctcaaa aggtattaca tagcctgcat tacctatttt tctaccattt tcagtagaaa
    25741 gaggaaactc catagatttc aaatttaaat ttcctatact taaaagttct tttcctaaag
    25801 atattatttc attaggattc atattagttt ttacatacgg aagtattgtg ttaactagat
    25861 taggatattt agtgactggt aaatccttaa caccatttat taaactctgt ataacacttc
    25921 tttgtcttct atctctttca tctgtactat cattttttct tatacgagaa aatgcaagtg
    25981 tctgatatcc atttaatttt tgctcaccag aatttcttat atatttaaga gaacctttat
    26041 tgtcattttt attccattta taagtttcag gaataaattt atttaattct cttatttcac
    26101 tcttatcaac agtaacagta actccaccta aagcatcaac tatatccata aaagagaaaa
    26161 agtcaactac ggcgtagttt tgtatatcaa gttggaagtt ttcttctata gtttctatta
    26221 ataagttagc ttgtccatat acgtaggcat gtgttaattt ttgttctcca tgtcctggta
    26281 tattaacata agtatctcta tttaatgaag ttagcttaag acttttattt ttattatcaa
    26341 tagtaagtat catcatggca tcagaacgag aaacttcttc tcctggtctt gcatctgtac
    26401 ctaacaataa tatgtttgtg atattttctt cgtttttata gtcattacta ctaagcgcct
    26461 ctgtatttat actatcatct tgtatagttt ttaatttagt aaaaacatat ccatatatac
    26521 taacaggaaa tgctataact aaaagggcta gaaatataac gaactttttg agtcctgaca
    26581 ataatatccc ctactttcta aattttttaa ttacacatag ttaattatat taaaaatttg
    26641 ttacataagc aataaaaaac acaataaatt agcataagtt tgtcataata taattatttt
    26701 tatagcattt tatagaagaa agtgtgtagt tattattgat tttattggaa tataattgta
    26761 tgaagactta aatttttata aactaatgtg tatattgaaa aaaatatttt cctgaatgta
    26821 gttatagcat cttatgaaag gtgcaaattg taatttaaaa tttttagtgt ataataagaa
    26881 ttgaaaattc aaaccgataa aaattaataa aaaataatta taaattaata atttggggga
    26941 ggaaatgtat gataaaatta atagcaacag atttagatgg aactttgtta gatgaaaaga
    27001 gcgaaataaa tccagagttt tataaagtat ttaaaaaatt aagagagaga gggataatgt
    27061 tttctgctgc tagtggcagg cagtatcaaa atctgataaa aaaatttgaa gatataaaag
    27121 atgatatgat gtttatatca gaaaatggaa cactagtagt atataaagga aaagaaatat
    27181 tatcaaatcc tttaaataaa gaattagtaa atgaaatcat agagacaaca agaagtataa
    27241 aagggaaaaa aattgtaatg agtgggaaaa aatacgctta tatagaaagc aaagatgaag
    27301 cgtttataca agaggttagt acttattatg caaagtttaa aattgtagaa gatttgacga
    27361 aagtagaggg agacatattg aagatagctg tgtttgactt caaaggtgca gaacataata
    27421 ataatatcta ctttgaaaag ttttctgata gagctcaagt atgtatttct ggagtggagt
    27481 ggcttgattt aactgcaaaa ggtgcaaata aagggagtgc cataaaaaaa gtacaaaaaa
    27541 tgttagatat aaaatatgaa gaaactatgg tatttggtga ccagttaaat gatgtggaaa
    27601 tgatgaaaag tgcctatcat agttatgcca tggaaaatgc aaatgaacat ttgaaacaaa
    27661 ttgctagatt tagagcaaaa agaaatactg aaaatggcgt tgtagataag ataaaagagg
    27721 ttataaaaat tggatagtca tatgtataga tgaaaaagat tattttgaag aaatcaaaaa
    27781 aatatcaatg taattttgat aaataagttt atttatgcga aaattacatt gatatttaat
    27841 aaacatcaat aatagatatt tattttttta tgatattcag tattttgttt tctatttgag
    27901 ttgcattttc agcagaaatt aaataaacat acctacctgc cttctcaatt atagtattat
    27961 ttgctatttc agaattgatt ttttctccat gtccttctcc aaaaggtgct tgtatcatat
    28021 cttttttata attttctaga gttttataga ttttatctac ctcgtcagtt tttataacga
    28081 taatatcttc tagagataat ttttcagaag aattaattac aaaaccttga attatgtctt
    28141 cctctacatc attaaaatag ttaaaatcct ttgcatcaaa gatttttgat tcctttataa
    28201 gtaaatcaga agattttaat gcttttttta tttttgatgt agaaacgttt ttgtaatcac
    28261 cacttgaaga acaactaacc attcctaatg ataagattat cgaaaaaaac aatattaaaa
    28321 tttttttcat gataaacctc caagtttttt aaactctgtc ttaaattagt ttactacata
    28381 attatataaa aataaatgta tttaatgtta cagttttgat taataatgta atattttgat
    28441 aataatacct attgagaggt gattagaaat gagaatacca aaacatgtag gaattatacc
    28501 ggatggaaat agaaggtggg caagtcaaaa tggtatgaca aaagataaag gatatgaaag
    28561 tggaatagac cctggactag agtcttatag attatgtaga gatttaggct gtgaagaggt
    28621 gactttttat ggatttacaa ctgataatac aagacgtcca tcagtgcaga caaaagcttt
    28681 tacaaaagca tgtattgatg ctgcaaatat attaatagat gaaggtgctg ctttattagt
    28741 tgtaggaaat acaaaatcgc caatgtttcc agaagaatta ttaccatata ctaatagaat
    28801 taaagccaaa gaaggtcaaa taaaagcaaa tcttctggta aattatggtt ggtattggga
    28861 cttaaataat atatcaggag tagaaaatgt aaataaaaat aatatacagg attacataca
    28921 gtcatttgat gtatccagac tagacttgat aatcagatgg ggtggaagaa ggagattaag
    28981 tggtttcctt cctgtacaat caatttatgc agatttttat gttatagata attattggcc
    29041 agattttaag agtgacgata taaaggacgc atttagttgg tatgatacac aagatgtcac
    29101 tttaggtgga taattaaaaa tattctgatt taaggttgta gagtgtttta aattattgaa
    29161 aaaaaaactt cttgatggta aaataacact aataatatgt aaaagtgtaa aaatttatac
    29221 caagaagggt gaagaaatgt caaaaggcaa taataacaat aattcaagaa acaaatctaa
    29281 aaagaccagt catctgaata gaaaaaagcg aaaattaaat aagaaaaagt tagcagtgtt
    29341 gatttgcttt acagttttat ttttatttat tgcatttaag gctactcaag gtgtagtagc
    29401 tttggtaaaa agtatggata aaagtaataa aactagtcaa caacaaaatg ttaactcaga
    29461 acaatttgac cttggtaatg aagaggaaaa taaaaagaaa aaatatactg tatttataga
    29521 cccaggtcat ggaggcaatg ataaaggaac tgaaagcaag accagtaata gatatgaaaa
    29581 agacctaaat ctacaaattg caaaaaaatt agcaaataaa ttatctaaac aaaaggatat
    29641 tcaagtagta gtatccagaa ctgatgatac atatatatca ttaaaggata gagctatctt
    29701 agcaaataat agttctgctg atgtgcttgt ttctattcat ttaaatgcag agaaaaatgg
    29761 taatacagca acaggtatag aaacttggta taggaataaa gctacagatg gttcaaaaga
    29821 acttgcacag acagtacaaa gtactatagt atcctatgtt aaagttagag ataggggaat
    29881 agttgaaaat aactttgaag tacttagaga atcaaatatg cctgctatat taatagagtg
    29941 tggtttttta acaacacctt cagaggaaca aaaaataata aatgaaaaat atcaagacca
    30001 acttgcggaa ggaattgttc aaggtgtact ttcataccta gattctaaag ggaataagta
    30061 aattaacttt actttaatgt gaggataaat ttaaaagttt aactgtgtaa attgtattaa
    30121 ctttatttcg taatgttgga aatattgttt gaatttagaa actatgaaat tagaaattat
    30181 tttatctata tctaatttca tagttttttt tgcaaaaaat ttatagttat aaaattttaa
    30241 aaaataaatt tagtataatg taatgtagtt atttatatgt gattttaact tgcattgatt
    30301 tgctatcaat tgtgctttat aattagtata tataaagttt aactgtaaga taatttaaaa
    30361 aataggggaa tttatatgaa taaaaatagc atgacaagaa tttacagttt aaataaaata
    30421 atttttatta taattggaat tttaggaata gttttactca caagacatat tgaggtagat
    30481 attaaagttt taattttaat tttattaacc ttgtttagta taaatgtatt tatttcatat
    30541 cttaaaatta agttgtatga ggataaaatt aatttaggat tgataaataa ttcagataag
    30601 tttataaata ttactaaaga tgagaacttc ctaaaacatg ttcaaaatta tattatcagt
    30661 aatgaaaaag aaaactgtgt aatggcttgt tttgatttat gtagactcaa acttataaat
    30721 gatatatatg gttatgagct aggggatgaa gtgttaaata atattttaag taacttaaaa
    30781 aattattttg gaaaagaagc tatttatgga aagttaaaaa gtgatgtttt tgtattaata
    30841 ataaagcttg aaaataggga acagaagatt ccttatttag taaatcttat taagaataaa
    30901 attgtgatac taagtcagat tgacagtttt aaaatggata taaatattga ggcttctatt
    30961 ggaatttata aatttaacaa taatgaaatt gatataaaaa aagctataga taatgctgat
    31021 atggcaaggc ttaaatcaaa aggactaaag cacatagagt atgtagaatt tgataatgct
    31081 atggaagaag aaatacaatc aattatgaaa atagagagag atttattctt ggctattaaa
    31141 aataaagagt ttgtaattca ctatcaacct aaagttgatt caagtacagg taacattata
    31201 ggttcagagg cattgataag atggttgcac ccatcattag gcatggttgg accaaataag
    31261 tttataccta tagctgaaaa aaatggtctg ataaacaata ttggtagatg gctaattcaa
    31321 gaagttttta ttaccattaa caagtggatt tcagaaggta taaatgtctt accagtctca
    31381 ataaatcttt ctagagtaga actatataaa aatgatttag ttgatttttt taaactgatg
    31441 tttaatacat ataatattcc aaaagaactt atagaattag aaattacaga aacgacagca
    31501 ctaagagatg taaaatttat aagtgaaagg ctttatgaga ttaaatcttt aggtatgaat
    31561 atatcattag atgattttgg agtaggtaat tcaaatttta tcaaccttaa ggggatacca
    31621 ctagatatta taaaaattga taggtcactt atattggata tagttacaaa tacaaaaaca
    31681 aagtttatgg tgaaagcaat tgttaacctt tcacatgatt taaacgtaac tgttatatgt
    31741 gaaggggtag aggatatgca tcaggtcaaa gtcttaagtg aactgggatg taatttcata
    31801 caaggttatg tattttttaa acctttggat gagatgaatt ataaaaaact tcttactgac
    31861 ggaagttata taaatctgaa agatactctt ttagataaat gcatgacagt tgaggaggag
    31921 taaggatgat ttcagaagat gtagattatt caataagaat cataagagct atatttaata
    31981 atggagaaga taaaccaata gatgctaaaa tgatattaaa aagtgaagat ataccaatac
    32041 taacactata cagactgctt agaaagcttt gtgcaaacaa tattcttact tttaacaaag
    32101 aagatggcaa gacatacatg ttaaatgtgt cattggatac aataagtcta agaactattg
    32161 tagaagtaat tgatggaaaa atatatataa ataaatgtac gagatatgta tctaattgca
    32221 gtaattgctt aaaaggttgt aaagtaaatc gtgaattgct agaaatagaa aaagaaattt
    32281 ataaactttt atcgagaaaa tctataagac aaatactaga ataaaaaatt ttcagctatg
    32341 ataataattg gaggttttcg gttatggatg atatatctca agataatttt ctactgtcta
    32401 aagagtatga gaatagctta gatgtagata ctaaaaaagc aagtggaata tattatactc
    32461 ctaaaataat tgtagattat attgtgaaaa aaactttaaa aaatcacgat ataattaaaa
    32521 atccatatcc acgaatactt gatatttcct gtgggtgtgg aaattttctt ttagaagtat
    32581 atgatatttt atatgactta tttgaggaaa atatatatga attgaaaaag aagtatgatg
    32641 aaaattattg gactgttgat aacatacacc gtcatatatt aaactattgt atatatggtg
    32701 ctgatattga tgaaaaagct ataagtatat taaaagatag cttaacaaat aaaaaggtag
    32761 taaatgattt agatgaatct gatataaaga ttaatctatt ttgttgtgat agtcttaaaa
    32821 agaaatggcg atataagttt gattacatag tgggtaatcc accctatatt ggtcataaaa
    32881 aacttgaaaa gaaatataaa aaatttctcc tagaaaaata tagtgaagtt tataaggata
    32941 aagctgactt atatttttgt ttttataaaa agattataga tattttaaag caaggaggga
    33001 ttggcagtgt tataactcca agatattttt tagaaagtct atcaggtaaa gatttaagag
    33061 aatatattaa atcaaatgtc aatgttcaag aaatagtgga ttttttaggt gcaaatatat
    33121 ttaaaaatat aggggtatct agttgtattc taacttttga taagaaaaaa acaaaagaga
    33181 catatataga tgtatttaaa atcaaaaatg aggatatatg tataaataaa tttgaaacat
    33241 tggaagaact tctaaagagt agcaaatttg aacattttaa tataaatcag agattgctat
    33301 ctgacgaatg gatacttgta aataaagatg atgaaacttt ttataataag atacaagaaa
    33361 aatgtaaata tagcttggaa gatatagcaa taagttttca aggaataatt acagggtgtg
    33421 ataaagcgtt tattttaagt aaagatgatg taaagttgaa ccttgtagat gataaatttt
    33481 taaaatgttg gataaaaagt aagaatataa ataaatatat agtagataaa agtgaatata
    33541 ggctaatata ctcaaatgat atagataatg agaatacaaa taaaaggata ctagatgaaa
    33601 ttatagggct atataaaaca aaattagaaa acagaagaga atgtaaaagt ggaataagga
    33661 aatggtatga acttcaatgg ggtagagaaa agcttttctt tgaaagaaaa aagataatgt
    33721 atccttataa atcaaatgaa aatagatttg ctatagatta tgataataat ttttcaagtg
    33781 cagacgtata ttcttttttt ataaaagaag agtatttaga taaattttca tatgaatatc
    33841 tagttggaat tttaaattcc agtgtttatg ataaatactt taaaataact gctaaaaaaa
    33901 tgagtaaaaa tatatatgat tactatccta ataaggttat gaagataagg atttttaggg
    33961 ataataatta tgaagagata gagaatttat caaagcaaat aatatcaatt ttattaaata
    34021 agtctataga taaaggaaaa gtagaaaaat tgcagataaa aatggataac ttgataatgg
    34081 attctttagg tatataagca gttgtaagta ttaaaaaatt ataatctttt aattgtttat
    34141 ttaaatttag aaataaaaag ttagccatat aaaaaatatt aaaaatatat acttttcaat
    34201 atttttttga taaaataaga atgaatttgt gcaattttat ttttctccaa taactaaaaa
    34261 tagttttttg aattaatatt tgcttaagaa taaaaatcag aaacgttttt gatatagtag
    34321 gtgataatat gaaaggatta gttttagaag gtggaggaac aaaaggagcc taccaaatag
    34381 gtgcatataa agctttaaga gagcttggta tagagtttca aggagtaact ggcacatcaa
    34441 ttggtgcttt aaatggagca tatataattc aaaatgatat tgaagtaatg gaagatattt
    34501 ggttaaatta tgactatact cattttatga atattgatag agaaacctat gagaggttta
    34561 aaaatgtaga tttcactact aaaaatctca atgcagtgtt tgagcttata aataaagcta
    34621 gaaaaaatga aggtatagat ataagcccat taagacaatt aatggagaca acattaaatg
    34681 aagacataat aaggaaatca aaaaaagact ttggacttgt tacggtctgg tgggataaaa
    34741 aaattaatcc atatccattg tatatcgaag atatacctaa aggaagattg attgattatt
    34801 taattgctag ttccagtttg cctatttttc aaacgaacat gatggatgat aagttgtatc
    34861 ttgatggaat gttttgtgat aacatgccaa taggactctt ggaagacaaa ggttatgaag
    34921 atgttgttgt aattaggttg attgatgatt ttattgggaa aatgaatcta aatcgacatc
    34981 aaaatataaa tataaaaact ataataccat cagaatatct aggaggttct cttaataaag
    35041 atagagataa tgtagaaaaa aacatccgac taggttactt agatactatg aaagcatatg
    35101 aaagatgcac tggaattaag tattattttg atgtagagta taaatataat gaagaatatt
    35161 gttttgataa atttagaagt atgagcaatg aaacaataca atatatctgt aatttattga
    35221 atataaaaag agatgcttca cttagaactg ttatggaaag tgtaatacca aagcttggtg
    35281 aggtgcttgg attgccaaag gatttttcat ataaagattt gttttactgt atatatgaaa
    35341 agaaacttga agaaaataat ataaacagga taaaattata tgattttaac aaggttatag
    35401 atgttgtaaa tacaaatatt gattctaata gaaatttagc ttctaatttt gaagttagac
    35461 ctaaaattag tattaaaaag aacaaaaaca caaaattatt gacaaattgt attataaaag
    35521 acataaaaaa tcaaagttaa ataaaaaaca aaaaaaaatt tgtaattata ctcgaaaaaa
    35581 gtgtatatta gtagtataat atttttgtag cactagtggg aacaaaaaat gaccatagga
    35641 caaaaaagta ccacttagta ccattaaagt aaaaaacagg aaaacaattt taaaaaatat
    35701 aaaatgtaaa gtttatagga ggatttaaaa aaatggaaaa agtagttagt atcaaaaatg
    35761 caagtggatt acatgctaga ccagcaggaa tgtttgttaa gaaggcagct gaatttaaat
    35821 caacagtaga ggttgtagca aaagacaaaa ctgtaaatgc aaaatcaata atgggaataa
    35881 tgagtttagg tttagctcaa ggagaagaat taaagttagt tgtaaatgga gaagatgaag
    35941 aagtagcttt agctgcaatg gttgaattag ttgaaagtgg atttggagaa tagatataat
    36001 aattttttaa aaatttgata tttagaatct aatattactt aaagtaaagt aacagagaag
    36061 tattaaacat atacagacca acatttgtag atatttaatt tctttgttac tttcatgttt
    36121 atttaatcag aagattaaga ctattgttaa ttgggtataa aaatataaat attttagaaa
    36181 atgaaataag atatttttaa tcataaattc agagtaaaaa tacagtaaca aatagtttat
    36241 aaaattttat aaaaaattat aagtaaataa aactataaaa atgtaaatta tttatataaa
    36301 tttataaagt ttatgtgcta gatgatattt tttaggaggt agagttatgg cttacaaagg
    36361 aataggtgct tctcctggtg ttgctttagg gaaagcgtta gtagttgaac atagtgaatt
    36421 agtaattgaa aaaaaatcta tagataatgt tgaagctgaa attgctaaat tagaagatgc
    36481 agttgcagtt tctaaagaag aattagtaaa agttaaggaa aaggcttctg aagaattagg
    36541 agcagaggaa gcagaaatat ttgaagcaca tttattagtt ttagaagacc cagagttaat
    36601 tggttctgca atagataaaa ttaaaactga aagtgtaaat gcagagtatg ctttaaatga
    36661 aataaaagag atgtttgtta gcatgtttga gtctatggac aatgagtaca tgaaagaaag
    36721 agcggcagat ataaaagatg ttacaaatag aatacttaga catatattag gaattaaagt
    36781 tgttgattta tctgctttaa gcgaagaagt tgtattaata gcacatgatt taactccatc
    36841 agacacagca actatgaata aaaaaatggt tttaggattt ttaactgata taggtggaag
    36901 aacttctcat actgctatca tgtctagaac tttagaaata gctgcaatcg ttggattaaa
    36961 tgatataact tcaaaagtta aggatggaga ttttgtggtt ttcaatggag acacaggtga
    37021 agtaatcgtg aatcctgatg aagagactat aaataaatat actgaattaa aagctaagta
    37081 tgaagatgag agaaaagcat tacagctatt aaaaggaaaa ccatctgtaa ctttagacgg
    37141 aaaacatgtg gaattagctg gtaatatagg aacacctaac gatatagaag gtctaataaa
    37201 gaatgatgct gaaggtgttg gtctttatag aacagaattt ttatacatgg atagagattc
    37261 tttcccaaca gaagaaatac aatatgaagc atacaaagct gtattagaag gaatggatgg
    37321 aaagcctata gttataagaa cattggatat aggtggagat aaagaattaa gttatttaag
    37381 tatggaacct gaaatgaatc cattcttagg ttacagagca ataagacttt gcctagatag
    37441 aaaagatata tttaagactc aattaagagc tttatataga gctagtgttc atggaagatt
    37501 aagaatcatg ttcccaatga tttcatcttt agaagagttg ttacaagcta aagaagttgt
    37561 aaaagaagtt ttagcagaac ttgattctga aggtgtggct tatgctaaag atgtagaaat
    37621 tggtatgatg atagaagttc catctgctgc tgtaatatct gatgtattgg ctaaacacgt
    37681 agatttcttc tctataggaa ctaatgattt aattcaatac acttgtgcag ttgatagaat
    37741 gaatcaaaag ataagctatc tttacaatca atttaatcca gctgttttaa gacttataaa
    37801 gactgttata gacaatgcac ataaagaagg caaatgggca ggtatgtgtg gagaatctgc
    37861 tggagaccaa aaaatgatac caatactttt aggtatggga ttagacgagt tctctatgtc
    37921 tccaatatca atacttcctg ctagaaagtt aataacttca gttaaagagt cagatatgaa
    37981 aaaattagct gatgatgtac ttaatatggg tactgctgaa gaaataaaaa gctatataga
    38041 aaagactttt aatatataat taatgttata tagttactaa aattcatttt tctaaataac
    38101 aaaattatat tttacgaata aaaaa
//
LOCUS       DAMJVI010000028        38016 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.029, whole genome shotgun sequence.
ACCESSION   DAMJVI010000028 DAMJVI010000000
VERSION     DAMJVI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.029"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            207..1424
                     /locus_tag="PC545_001521"
     CDS             207..1424
                     /locus_tag="PC545_001521"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HDF2456293.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKGYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDKYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKIVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1519..1899
                     /locus_tag="PC545_001522"
     CDS             1519..1899
                     /locus_tag="PC545_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2456294.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2183..2665
                     /locus_tag="PC545_001523"
     CDS             2183..2665
                     /locus_tag="PC545_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2456295.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNSINIEKVNDSVFSKKLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVSLQGEGFKNEVSQGD
                     TVKKGSPIISFEREKINSQGIDCTTIIIVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2686..3501
                     /locus_tag="PC545_001524"
     CDS             2686..3501
                     /locus_tag="PC545_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2456296.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKVNEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEIIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSIDLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNVYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3554..5104
                     /locus_tag="PC545_001525"
     CDS             3554..5104
                     /locus_tag="PC545_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2456297.1"
                     /translation="MKEKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     LPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQLAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGYIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGADTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSVIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEEQSKQLNLDAGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEAY"
     gene            5201..6553
                     /locus_tag="PC545_001526"
     CDS             5201..6553
                     /locus_tag="PC545_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2456298.1"
                     /translation="MIITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKKRQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKDYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLESFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6684..7442
                     /locus_tag="PC545_001527"
     CDS             6684..7442
                     /locus_tag="PC545_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456299.1"
                     /translation="MNIINKIFWQEDRIGMITNNLEADTHSHCMLQLFLGIEDSIEIT
                     VNEKLVKCNCIIVDKNISHSFSARKKVYYSAIIEPTSIYAEQLTSKMNDFGYWICDND
                     GLEKLRQQGTFLISNSSKEQYLRFMEMLNNYLNIPITLKHYDDRITELFNLLHTCNCD
                     NHTISSFADKVSLSASRLSHLFKEQIGIPLKSYILFHQMECAFRELLSGKNVTEASMT
                     AGFDTPSHFAGTVKRMMGMPVSLSLKDSEFLKVY"
     gene            7489..8208
                     /locus_tag="PC545_001528"
     CDS             7489..8208
                     /locus_tag="PC545_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2456300.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKNWLELDDFHKIKSIYEFV
                     QNDILFGYNASDMLSATQVLNDGMGQCNTKATLLMALLRAVNIPCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFFNHGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8332..9732)
                     /locus_tag="PC545_001529"
     CDS             complement(8332..9732)
                     /locus_tag="PC545_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDF2456301.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLIARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLFLSPIAILDVILSFILLRNVGETQKLKLDIPSIVLSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPILIGIMSLILFTLRQLKSKEPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPIAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLIHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINNTLRQISGAIGSAILITIMTSATKKAHMSSNMLSNIHGIDVAFSIAATLAFTG
                     LIVSICFIKRHQIIRS"
     gene            complement(9736..10644)
                     /locus_tag="PC545_001530"
     CDS             complement(9736..10644)
                     /locus_tag="PC545_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456302.1"
                     /translation="MKGDNKVTEKQRLIITIAQKIFDQKGFQNTSISDVVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPASEKLNRKIIAVWKNIFSRT
                     TINTYIRENFSEEQRKATSKLQTKSRANLLNEYKLSLFDNYGDKIEKIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFIAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEVYF
                     SLHNKSLFFKMIQDIEELIKTDTSLLENPKLLEAIKKLYVEGKNQQYDSLIMDAMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10905..11552
                     /locus_tag="PC545_001531"
     CDS             10905..11552
                     /locus_tag="PC545_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2456303.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARDAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGDDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKENITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11876..14515
                     /locus_tag="PC545_001532"
     CDS             11876..14515
                     /locus_tag="PC545_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2456304.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQSLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGAVVVYGRGSFVVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMAGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEEDFIPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     EGDLALTGKELDALSEEQLYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSIVFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLKAVAIRGCIIGVVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14573..15676)
                     /locus_tag="PC545_001533"
     CDS             complement(14573..15676)
                     /locus_tag="PC545_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HDF2456305.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFIALDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIHLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVSPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQIIDNIE"
     gene            16037..16858
                     /locus_tag="PC545_001534"
     CDS             16037..16858
                     /locus_tag="PC545_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2456306.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKSGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTRTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIVKDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16937..17148
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17235..18551
                     /gene="argH"
                     /locus_tag="PC545_001535"
     CDS             17235..18551
                     /gene="argH"
                     /locus_tag="PC545_001535"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDF2456307.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCVLYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLVLDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVGLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELHKFSDAFKEDIYDFIDYESILNKGIKKNLK"
     gene            18800..19138
                     /locus_tag="PC545_001536"
     CDS             18800..19138
                     /locus_tag="PC545_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HDF2456308.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
     gene            complement(19265..20554)
                     /locus_tag="PC545_001537"
     CDS             complement(19265..20554)
                     /locus_tag="PC545_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2456309.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPAIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKDLPQIDGSSMYLKNGE
                     SFTVKQLLDALLVHSANDAAFVLARYVGGGNVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELIYEPKNSYRIFLEKDESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNVYNNGKLENSIDLIAKNNLDSSLPFFTGNNILMT
                     FVKIIAGILVLLVLFIIASNIKKKKRKKARSKKNMKK"
     gene            20634..21338
                     /locus_tag="PC545_001538"
     CDS             20634..21338
                     /locus_tag="PC545_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDF2456310.1"
                     /translation="MKKKVKFISFFVLFILIISSIIVYKSLIFKDDIYVVSKKNLPNE
                     KTHVVENEIKGEDNNHNKVTKDIDKNFQNNKITIYISGAVNRPGIVTIESDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEATSVTSNDDSNKPNQRNESRGEPK
                     NKSNKNISNESKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDLICVD"
     gene            21430..22476
                     /gene="selD"
                     /locus_tag="PC545_001539"
     CDS             21430..22476
                     /gene="selD"
                     /locus_tag="PC545_001539"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:21478..21480,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDF2456311.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFDKVKESGALLVGGHTVDDKEPKYGLSVSGIVSPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFEK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEVVGNIEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            22494..23906
                     /locus_tag="PC545_001540"
     CDS             22494..23906
                     /locus_tag="PC545_001540"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDF2456312.1"
                     /translation="MIVLSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDASNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVISITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVEELRKAFN"
     gene            24020..25903
                     /gene="selB"
                     /locus_tag="PC545_001541"
     CDS             24020..25903
                     /gene="selB"
                     /locus_tag="PC545_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDF2456313.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNENSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIESGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKRALETLISEDKVFIIGNMYMHINQYNKLKENTIKLLS
                     EYHKKYRLRKGILKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNDKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNILINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            26008..28746
                     /locus_tag="PC545_001542"
     CDS             26008..28746
                     /locus_tag="PC545_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HDF2456314.1"
                     /translation="MKNIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDIEKYETLL
                     HAIYTIFLVATGVIIFNTTKFKVDSFSTFLGLIFIATGVLECIYLFNSLGIKTKSIME
                     INITISAITDLFPILGVYLSFKFVKDNKQIYSSVILFIATATLTITSLFIISGICDYL
                     GNGKSQYALGVIISIFIIIISIISGIELNSSSNKGKWEYDEKKIFNRIIVIIILSRVP
                     NLLHIVIDNRNVERILSQIIINVALYYLYNYIVSKNIKKTALELHDTNEELTKKTESL
                     KEKNKKLIYETEKIEELKGILMKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESIHYVFVHEKNAEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSKDFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTNKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYDDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRNEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTDLEEINVYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVVSEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(28865..29809)
                     /gene="manA"
                     /locus_tag="PC545_001543"
     CDS             complement(28865..29809)
                     /gene="manA"
                     /locus_tag="PC545_001543"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDF2456315.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGKYKGKKLSELWNKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVNMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNSGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFKCTTFVSNEFFSVYKLDVFGKCNFTHNTPFSLYSVLDGNGK
                     LIHNSVEYNLKKGMHFILPNDFGDFSFEGNLEIICSHI"
     gene            29982..31343
                     /locus_tag="PC545_001544"
     CDS             29982..31343
                     /locus_tag="PC545_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2456316.1"
                     /translation="MELFKAESKKYKKEFLKLLEQWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLISNIDDKSLYISGGIEFNTIPDKVYI
                     KNVEKLGKDNICFDINNISINYDNGNYIVYGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSVRYPEIL
                     TIEDIIKKFNLYMEQNNINKFELIGENLKQANYIDRNSKLVRSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHSANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            31431..33320
                     /locus_tag="PC545_001545"
     CDS             31431..33320
                     /locus_tag="PC545_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2456317.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIASSRGIGYKLSGSKNNIARAVKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIHCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIENLMKEISDKIGFKLIEDEVIYISNYSGV
                     IAYKGTQGRKNTSEIEERISTVISSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFSIKASTK
                     EEVIISLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            33335..33790
                     /locus_tag="PC545_001546"
     CDS             33335..33790
                     /locus_tag="PC545_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2456318.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKDLNLAEDESLVY
                     EGLMAREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKEFKDSLLKSDNVEEISNLINEALGL"
     gene            33849..34166
                     /locus_tag="PC545_001547"
     CDS             33849..34166
                     /locus_tag="PC545_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2456319.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            34191..35453
                     /locus_tag="PC545_001548"
     CDS             34191..35453
                     /locus_tag="PC545_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2456320.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKRLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAIWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            35558..36622
                     /locus_tag="PC545_001549"
     CDS             35558..36622
                     /locus_tag="PC545_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2456321.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     ESQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIVDFIKVGMTE
                     KQVENEIVRIIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITQDGCMRLTTSTKDLIVVS"
     gene            36676..37854
                     /locus_tag="PC545_001550"
     CDS             36676..37854
                     /locus_tag="PC545_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HDF2456322.1"
                     /translation="MYNFDDKPDRVSEKCRKWDLNIIRDKFGDIREDFIPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVNVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEVQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTRVATICKENNVILVADEVHAEHIHYGTFNS
                     ILKIGKELLENVILLTSPNKGFNLGGLKTSYSIVINKDIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLSGVNEYIKSNYELLETWVNKYNKIKLMKMESSYLAWMDISGL
                     GISASEFTDKLAIDTGVLLEDGSHFVKDGEKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT        14931 a         4157 c         6661 g        12267 t
ORIGIN      
        1 gtaaaaaata tttttaaatt atcatttaat gtaatttaaa tgaattgaag atatatgttt
       61 attgtatgca attgttgtat ttttaggttt tttgtgatta attaaaaaat tataaaaatc
      121 gtttacatgt tgatttttaa aaaaaaaaga tatagaatta tatagaaagt aataaacaaa
      181 aattggattt acgggagtgg aatgaaatga tgaatgttac cttaacagat gtaaaagagg
      241 ctagagagac cattaagaac atagtaaaaa gaactgactt acttgaaagt gttaaactta
      301 gcgaaaaaac aggagcaaat gtgttctata aatgtgagaa tcttcaaaaa acaggttctt
      361 ttaaattaag aggagcttgt aacaaaatag cgagtttaac agatgaagaa aaggcaaatg
      421 gagttatagc ttcaagtgca gggaatcatg ctcaaggagt tgcattggga gcaaaaatga
      481 ctggtataaa atctacaata gtaatgccag caactgcgcc tttagcaaaa gttagtgcaa
      541 ctaaaggtta tggagcagaa gtagttttaa atggtgcggt ttatgatgat gcatatgcaa
      601 aagctgttga aatacaaaaa gaaacaggag caactttctt acatccattc aatgataagt
      661 atgttatagc aggacaagga actatatctc ttgaaatttt cgaacaatta gacaataaag
      721 ttgatactat actatgccca gttggtggag gaggaatcat ctctggtgtt gctgttgcag
      781 caaaagcatt aaatcctaat gtaaaaatag tcggtgttca aactgcaaat ataccttcaa
      841 tgaaagagtc aataaaaaat ggaaaagtaa caacagcatt taatgatact acaatagctg
      901 atggtatagc tgttaaaact ccaggagatt taacttttga aataataaat gaattggttg
      961 atgaaatagt agtggttgaa gagacagaaa ttgcagaatc aatattattt atgatggaaa
     1021 gtcaaaaaat agtttcagaa ggtgctggtg ctgtatgtac agctgctata ttaagtggta
     1081 agtatgtacc agcaaaagat gaaaatgtag tttgtataat atctggtggt aatatagaca
     1141 taaatacgtt atatagaata ataggtgttg ctttagctaa agaaggaaga agatattcat
     1201 ttagtacaat tatggaagat aagccaggaa actttgctga gttaactaga ataataagtg
     1261 agaatggtgg aaacatttta agtgctaatc aaggtaaatt atcagcaggg gaagctctag
     1321 gaaaacaaag tgcggaattt atattagaga caatagatta tgaccatata gctagaataa
     1381 agaaagctat agaagaaaaa ggttttaaaa ttatagaatt ataataaaaa gtgtatataa
     1441 tatacaagaa aaatagtata atagtgtatg aagatacatt ttatgtgtat tagattaacg
     1501 aattaggagg attttaaaat gaaacatgaa gtaatacata ctaatgatgc accagcagct
     1561 ttaggaccat attctcaagc tataaaagct ggaaacttgt tatttgtatc tggacaagtt
     1621 cctcttgtac cagagactat ggaagtagtt gaaggagatg tacaagctca aactgctcaa
     1681 tctttaaaaa atcttaaagc tatattagct gaaagtggag ctgatttttc aaatgtagtt
     1741 aagactacag tatttataaa agatatgaat gaatttgggg ctataaacga agtttatgct
     1801 gaatattttg gagaaaataa accagcaaga gcttgtgtag aagttgctag attaccaaaa
     1861 gacgttaaag ttgaaataga agtaatagct gtattataaa aatacaatat ttttaatttg
     1921 aaaaggctga aaaactatat tctagtgatt cagccttttt ataaaaaaat aaatcacaat
     1981 aaagaacatg ataaatattg tcgaattaaa aaataaaatt aaaataaata ttgcaatatt
     2041 tttgagaagg ttttataatg agtatataat ttgaaacgtg taactattaa attaggcatg
     2101 agttcttgga tatttttatc ctgaggattc atgccttttt tgtattgaaa ttcaaaaatc
     2161 tatttaataa ggaggaagta ctgtgttaaa ttttttcaag aaaaataaaa gctataagtt
     2221 acatgcagta gtgagtggta acagcattaa tattgagaaa gtaaacgatt ctgtgttttc
     2281 aaaaaagtta atgggagatg gagttgcaat tattcctaat agtaatgttg ttgtagcccc
     2341 ttgtaatggt aaagtaactg tgcttacaga atcaaagcat gcctttggta tggtctcaga
     2401 tgagggtgtt gaaatattag tccacatagg aattgataca gttagtttac agggtgaggg
     2461 gtttaaaaat gaagtgtctc aaggagatac tgttaaaaaa ggtagtccaa taataagttt
     2521 tgaaagagaa aaaataaatt ctcagggaat tgattgtaca acaataataa ttgtattaaa
     2581 tcatagcgaa ttttcagaaa taaattgcat ggtagaaaat gaagttgttg cagggcaaga
     2641 tactgttatt gaaattatga aataatagaa agggattaat tcaatatgag agtagtaaaa
     2701 atctttaata ataatgcatt atctacagta actaatgata ggaaagaagc tattttacta
     2761 ggtcttggta taggttttaa taaacgtcca ggagataaag ttaatgaaga taaaatagaa
     2821 aagatttatt atgttcaaga ccacatgcaa acaaaatttt tagaattatt aaaaaatgtt
     2881 agtccagaag tgatggatgc ttcacaacaa attataagtc ttataccagg aaatgaggga
     2941 aaatttaata ataaagggat attatcttta gtggaacata ttagttttgc aatagataga
     3001 atgaaaaata atgttttttt acctaattta atgcttttgg atataaagat gatgtattca
     3061 aaagagtttg aattaggagt aaaagcttta gaaattatat atagagtatg tcatatccat
     3121 ttgcctgaag atgaagcagg ttacatagct ttacattttg tcaatttaca atcaaatgat
     3181 aatttggcat atgatacttt aaaatttgta aaaggtagta ttgatttgat taaagaatgt
     3241 tatggattag aattagacga atcgagttta agtacattac gttttagaac tcatcttaaa
     3301 tttcttgctc aaagaatttt taagaatgaa atttgtcagg atgataaaat gattgaaatg
     3361 tatgattatt taattaacaa tcatcctaaa aataaagaat atttagaaaa gttaaatgta
     3421 tatattgaaa aggagtttag gtataaatta gataaacctg aaaaaattta tctactacta
     3481 cacctaacaa aaatcttata agaatgagat gaattagtaa tagaaatata tataatttta
     3541 gggggggata agattgaaag aaaaactact tggaggactg gaaacatttg caaaggcaat
     3601 ggttcagcca ttaatgtatt tatcgagtgc aggaatatta atggtggtag gtgtactact
     3661 tacaaatgat atgttattag gagtacttcc attttttaaa tgggagccaa ttcaaatatt
     3721 tggaagccta atttataact gtataatggc tattattaat aatcttgctg ttatatttgc
     3781 tataggtatt cctgctgcac tagcaaaaaa agagaagcac aaagcagcgt taataggatt
     3841 tatgagttat ataatatacc ttacagcttc caacactatg ttaacatcac taggtcaact
     3901 agcaaaagaa agcaagatgc ttggactgat agggactgga caagctacta ttttaggtat
     3961 tcaagttgta gatacaagtg tatttggtgg aattatttta gggtatatag ctggatatgt
     4021 atttaatcgt acaagcaaca aaacatttaa aggagcttta cagatatact cgggagcaaa
     4081 ttttagtttt atatgcatgt tctttgtatc cattttaatt ggagttgtga gtaactttat
     4141 atggccatta gctcaacaag gaatagcttc tcttgcaaat gtcatcaaag atacaggtac
     4201 atttggttta ttcttatatg gttttttaga gagaatatta gtaccaactg gacttcatca
     4261 tttaatttat acaccatttc agttttctga tttaggagga gtgcttaaag ttggtgcaga
     4321 tacagtttca ggagcctatg ctatagttat gacagaaatg aatttaccag ttgagcgttt
     4381 ctcagatagt atatactaca tggcaactgg atttacaaag atgtttggtt atataggtat
     4441 aggagctgcc tttatttata ctgcatataa gcaaaataag aagaaggtta aggcaacgat
     4501 tattccacta ataattacgg cagtattagc ctctgtaaca gaaccattag actttatgtt
     4561 tgtattctgt gcaccacttt tattctttgt acatgcagtt atagctggtc tttttatagc
     4621 tttattaaaa atatttgatg ttactgcatt atgtggaggg aacttattag tatcagttat
     4681 aatgaatact acagtaggcg tagaaaaaac acactggcca gtaatgatga tattaggact
     4741 tgcacaaatt attgtatatt ttgtagtatt tagtttttta attaagaaat ttaattataa
     4801 aactccaggt cgtgaagatg agagtacagc tacaggcgaa gaacaatcaa aacaacttaa
     4861 tttagatgca gggatagaaa atataattga tgggttaggc ggaaaagaaa atattaatac
     4921 agtagaaaat tgcatcacta gattacgtgt taatgtaaaa gatgaaagta agattaatga
     4981 agatataatt aatttaacgc caaatagcgg gattgtaagg aaaggaaaag atatacaaat
     5041 aatttttgga cttcatgtgc atgaggtaag acgtgcagta gaagattttt tagaagctta
     5101 ttaaagatat atatttatat agcaagggta aataacagct ttaagtgctg tttactattt
     5161 attaaagata catataattt atagaggagg aataattatt atgattatta ctattgttgg
     5221 tggaggaagc acatttactc caggtattgt taagtcaatt gcattaagag aaaaagagtt
     5281 agagcttgag gaaattcgtt tatttgatat tgataaaaaa cgtcaagata aagtagctgt
     5341 agttgtcaac tggatactaa aagaagatgt taaaacagat gttaaactaa ctgtaactaa
     5401 ttgtgaaaaa gaagcgtata cagatgctac atttatattt gcacaaatgc gtgttggtaa
     5461 atatgaaatg cgtgagcagg atgaaaaaat tccattgaaa tatggttgtg taggtcaaga
     5521 aacttgtggt tgtggaggga tggcatacgg actgcgtaca atacaaccaa tgataaaggt
     5581 tattgatgat gtagagaaat atgctaaaaa agattattgg atattaaatt actctaatcc
     5641 tgctgcaatc gtttctgaag cttgtcgtaa gttaagacca aatgcaagga ttataaatat
     5701 ctgtgatatg ccaatagcta ttatagatat gatagcgggg tcattaaata tcaatgacgt
     5761 tcataatata cgctatgatt attttggtct aaatcatttt ggctggttta cttcaataga
     5821 ttataaacat agagacttaa tggaagagat taaggaatac ataaaagaaa ataaaatatt
     5881 attaccaaaa tcttatatag aaaacttaaa taaaagtcgt agtacaggaa atcgccatgc
     5941 tgtaagtagc tggatacatg tttgggaatc tgtttataca atgttagaaa gcttcccaaa
     6001 atatctgcca aacacttatt taacatacta tcttttaagt aaagaaacag tggaacattc
     6061 aaacataaat cgtactcgtg caaatgaggt tatggaaagc cgtgaaaaag acttatttga
     6121 aggtgtgaga cattacttag aaactggaga aatttctgaa aaagcatttt atgcaggttc
     6181 acatggagat tggattagtg atttggcagt ttcaattaaa aatgatacac gtagtcgttt
     6241 tttagttata actgaaaatc gtggagctat accaaacatg ccatatgatg caatggtaga
     6301 aattccagca tatattggta aagatggtcc agaagttatt gctcgaggtc caatacctct
     6361 atttcaacaa ggattaatga tgcaacaact taacagtgaa aaattgatag tagaagctta
     6421 tatagaaaat agttatgaaa aagctttaca agcattcaca ttaaataaaa ctattccaag
     6481 tatgaatgtt gctaaagaaa ttttggatga aatgatagaa gttaataagg aatactggcc
     6541 agaattaaga tagtaggata aaagaaaggg tttttattta atcattagat atgttttcag
     6601 agtcttttat caaaatgtat tcacaaacat atggcaatat attaaaataa agagtgatat
     6661 ttttagaaga atggggtgat gcaatgaaca ttataaataa aattttttgg caagaagata
     6721 ggattggcat gattacaaat aatttagagg cagatacaca tagccactgt atgttacaac
     6781 tgttcttagg gatagaagac agcattgaaa taacagttaa tgaaaaatta gttaaatgta
     6841 attgtatcat agtagataaa aatatctctc attcattttc agcaagaaaa aaagtttatt
     6901 actctgctat tattgaacct acatctatat atgcagaaca gcttacctca aaaatgaatg
     6961 attttggcta ctggatatgt gacaatgatg gattagagaa attacgtcaa caaggaactt
     7021 ttttgatatc taactctagt aaagaacaat atttaagatt tatggaaatg cttaataact
     7081 atttgaatat acctataact ctcaaacatt atgatgacag aattactgaa ttgttcaatt
     7141 tattacatac ttgtaattgt gataatcata ctatttcaag ttttgcagat aaagtgtctc
     7201 tatcagcaag tagattatca catctgttta aagaacaaat agggatacct ttaaaaagct
     7261 atatcttatt tcatcaaatg gagtgtgctt ttagagaatt actttctgga aaaaatgtta
     7321 ccgaagcttc tatgactgct ggattcgata ctccatcaca ctttgcaggg acagttaagc
     7381 gaatgatggg aatgcctgtt tctttatctt tgaaagatag cgagtttttg aaagtatatt
     7441 aaatattctc gttatataat gaagttgtac taagaaaggg gctttgttat gaataaagaa
     7501 tatttagaag aaacaaaaat gttaaattat aatgaaccac aacttaagct tttggtatca
     7561 tcaaagaatt ggttggaatt agatgatttt cataaaataa aatctattta tgagtttgta
     7621 cagaatgata ttttatttgg atataatgct tctgatatgt taagcgcaac acaggtatta
     7681 aatgatggta tgggacaatg caatacaaaa gctactcttt taatggcatt attacgtgca
     7741 gtgaatatcc cttgtaggct tcatgctttt gatgtaacaa aagattttca acgaggagct
     7801 acatccaaat tgatttcact attagcacca aaatatattc ttcatacttg ggtagaggta
     7861 ttttatcaag ataggtggat tgctctggag ggtgtcataa ctgataaaaa atatttagaa
     7921 gcaattcaga agaaattttt taatcatggt ggaacgttta aaaagtatgc aatagctaca
     7981 aatgatttga aaaatacatc tattgattgg gatggtaaag atacttttat ccaaaaagaa
     8041 gctatcgtgt atgattatgg tatttttcca tcaccagacg tatttttttc tactcattca
     8101 caacatatgt caaaattaaa aaatttcata tatgtacatc ttatcagaaa aataatgaca
     8161 aaaaatgtat gtaaggctag aaacaattat attgataaaa atgaatgagt gtttaagagg
     8221 taactatata ggaaatatat agaataatta aaactgtact ggttaaaatg aataaaaagg
     8281 agttatcaca gagcaaactt aaattgttca taggataact ccttttctgt attaacttct
     8341 aataatttga tgtcttttta taaaacatat agatacaatt aaaccagtaa atgcaagagt
     8401 agctgctatt gaaaatgcaa catctattcc atgaatgtta gacagcatgt ttgaactcat
     8461 atgagctttc ttagtagcac ttgtcattat agtaataagt atagcagaac ctatagctcc
     8521 agatatctga cggagtgtgt tattaattgc tgtggcatga gatataagtt ctctatctaa
     8581 tgttttaatt ccccatgtag ttaatggcat taaaaccatt gaaataccaa tcatacgaat
     8641 acaatacatc aatgacacat gaattaaaga tgtatctctg cctaaaaaag cgaaggataa
     8701 agttcctaaa aacaaacatc ctgttcctaa tattgataga gcatgaggtc catatttatc
     8761 taaatgacgt cctgcaattg gattgagtac aaccatcaat atagcaccag gtaacattaa
     8821 tgaacctgaa gataatgcag aaaatcctct tacagattga acgtacagag gaatcattaa
     8881 tgtagcagac atcattgagg catatacaat cataattaat attgtagaaa taagaaatgg
     8941 tttattttta aaaaccctca attctaaaaa aggttcttta gattttaatt gacgcaatgt
     9001 aaataaaatt aatgacataa ttcctattaa tattggtaaa tatgtggcaa tattagtcca
     9061 cccataatta ccttgattag taaatccaat taataaacca ccaaaaccta gagatgatag
     9121 tactattgaa ggtatatcta attttagttt ttgagtttcc ccaacatttc ttaataaaat
     9181 aaatgatagt ataacatcta atatagcaat aggggaaaga aacagaaata aactatgcca
     9241 accaaatgaa tctactaacc atcctgataa agttggacct attgctggag caaatcctac
     9301 tgtaatacca actaaactca tagctgcacc acgtttttcc tcagggcata aatagagtat
     9361 aataacttgt aaaagtggca ttagagaacc agaacccatt gcctgcaaca cacgagcaat
     9421 caacatagtt gaaaaattat tagaaaaaag tgcaataata catcctatag aaaagataga
     9481 cattgatgta ataaataact ttctagtact aaatcgattt attaaataag ctgttgtggg
     9541 tacaataata cctgcaataa gtagataaat tgttgttaac cactgaccaa catcagcagt
     9601 aattttaaaa tctgacatta tattaggtaa tgctgttgac aatagtgttt gactgagaga
     9661 acttataaaa gcaccagtca ttacaattgc tattattaaa tttccttttt tattgttact
     9721 ttgggtttcc ataatttata acgtctcctt ttttaattga tttttaatgc tgttcaataa
     9781 aagtactttt ggtcttaaag tgtcttcttt ttctaaatat gcaatcattg catccataat
     9841 taaagaatca tattgttgat tttttccctc gacgtataat ttttttattg cttctagtaa
     9901 tttaggattt tcaaggagag aagtatcagt tttgattagt tcttctatat cttgtatcat
     9961 tttaaaaaat agcgatttat tatgaaggga aaagtaaact tcatcttctt gtttatgtaa
    10021 aaacattgat ttttcaatta aaggatttag attatccata ttttctacaa ctaaatctag
    10081 aatttgtata gtaaatttgg caataaagta tacatttatt tctctttttt gtacatgaat
    10141 aatgtatata aactgatgaa ttagagcatc caataagaaa actaaatcaa atataatttt
    10201 ttctatttta tcaccatagt tatcaaataa gcttaatttg tattcattca ataagttagc
    10261 tcgtgatttt gtttgcaatt tacttgtagc cttacgttgc tcttcagaaa aattttctct
    10321 tatataggta tttatggtag ttctagaaaa aatatttttc catacagcaa ttatctttct
    10381 gtttaacttt tctgatgctg gaatgtcatt attttcattt atggaatgta taatttctaa
    10441 aaacttttca tcataataat tgattatttc gcagataaaa ctttccttag tttcaaaatg
    10501 cttgtaaaaa gtagctttag acattttaca ctcttttaca acatccgata ttgaagtatt
    10561 ttgaaaaccc ttttggtcaa aaattttttg tgcaatagtt ataatgagcc tttgtttttc
    10621 tgtcacttta ttatctcctt tcacgcaaaa acgtttagta ctatagagta cctacaagtt
    10681 ttaataaata tattatacat catttggaaa aaaaatcaat atatagactg taatatgaaa
    10741 tttttgaaaa atttgttgtt atttttagtc gtataaaaaa gaaacttaag agtttcttaa
    10801 gaattgttta aagaagttgc aataaattag tggtatgatt atagtatgaa agagaatgtc
    10861 ttgaaatttt tctgtatcaa attccaatta tggaggtgta gtatatgaaa aagaaaattt
    10921 gtgtagcaat aatttttgct ctatttatag tttttgtaga aatgtttatt gatagctcta
    10981 gcgaaggaag taaaattatg acagctgaat taagtaaata caggaaaggc gtaaatgtat
    11041 tatcaataaa caatattata gtagcgaata aaaaatatag tttgcccaaa gattattctc
    11101 ctcaggaaag cagtgaagcg agagatgctt tttataaaat gaataaagat gctcaaaagt
    11161 caggattaaa tttaaaagct tttagtacat atagaagtta tgaatatcaa gatagattat
    11221 ttaagtcata tgtaaaagaa catggagaaa aagaagcaaa tagattttca gctaaacctg
    11281 gagagagtga gcatcaaact ggacttgctt ttgatattgg aggagatgac cagtcttgtt
    11341 gggcaaataa aaagttcaac aatacaaaag aagctaagtg gttgtatgaa aatgcatata
    11401 aatatggttt tatacttaga tatccagaag gtaaagagaa tataacagga tatatgtatg
    11461 aatcttggca ctatagatat gttggaactg aacatagcaa aaactttgca atgaataatt
    11521 tgactttaga agaatattta catataaatt aagagatggt ttgttaaaat taggttaaaa
    11581 taaaaaataa cctagactat tagctgtgta aagtgatata atgtaaatac aatttagata
    11641 actactcgaa ggggagtagc ttacacgaaa gtggtaatta atcgtcaaga cagtagaaat
    11701 actcggttaa ttagcaaggg attccttgat agcaagacct tcaaaataag cattaggtat
    11761 gtttgttttg gggtcttttt ttattcaagg aatttttatt taaaaaaatt taaatttcaa
    11821 gtatacataa gagcccccaa acataaaata aatttattga agaggagggc aacaaatgtc
    11881 ttacaaaagt acttatcaag aagtaattaa aaatttagac tcaagtttat ctggtttgag
    11941 taaagaaaga gcagaagagt tgttagaaaa aaatggagct aatgaattaa aagaagcaga
    12001 taaggttcct acttataagc ttttcctaga aagttttaaa gacccattag taataattct
    12061 attgatagct gctttagtac aaatattttt aggtgaaaca gtggagtcta taataatttt
    12121 tgcagttatt attataaact ctgttctggg tgttgtacaa acaaaaaaag cagaaagttc
    12181 attagaaagt ttgaaaaatt tatctgcacc aaacgcaaag gtaataagag acaataaaaa
    12241 aatgacaatt ccagcaaaaa atctagtagt tggagatatt gtattcttag aagctggcga
    12301 ttacatacca gcagatggaa ggcttataga agcacaatct ctcaaggttg tagaagggat
    12361 gttaactgga gaatcagaac cagttttaaa gcatacagaa aaaataagtg aagatgtaag
    12421 tttaggtgac cagaaaaata tggtatttag tggtgctgta gttgtttatg gtagaggttc
    12481 ttttgtagtg actggaacag gtatggatac tgaaatgggt aaaatagctg ggcttttaga
    12541 aagtgctgaa aataaactta caccattgca acaaaaaata gaggatttta gtaaaaaact
    12601 tggtgtaggt attgtaatac ttgctctttt aatatttata attcaagtag caagaaatta
    12661 ctttatggct ggaaatggag aaatgacaca aattatttta gattcattca tgttctctgt
    12721 agcagtagca gtagcagcta taccagaagc tctatcatca atagtcacta tagtattggc
    12781 tgttggaaca aatagtatgg caaaaaaaca agccataata agaaaattac cagcagtaga
    12841 aactttaggt tcaacaagtg taatatgtac tgataaaaca ggtactctta cacaaaataa
    12901 aatgacagta gttgattatt atatgtatgg aactaataat gatgagttag ataaagaaaa
    12961 agtaaactat tcttatcatg ctaaattatt gactatggtt tctacattgt gtaatgactc
    13021 tcatataact agtgatggta aagaagttgg tgacccaaca gaagtagctt taataaatta
    13081 ttcaagtaaa aatgatttag attatgataa attaagaagt aagtatatca gaataaatga
    13141 aataccattt gactcagaca gaaagcttat gtctactgta aatagtatat atggagatta
    13201 tataatgttt acaaaaggtg ctccagatat tgtgtttagt agatgtaaat atgctttgaa
    13261 aaatggttca aaagtagata taacagatga gataatagaa gaatataaac ataaaaatga
    13321 agagttttca aatagagcac ttagagtatt agcttttgca ataaaagatg tcccagaaga
    13381 agattttata ccttcaattg atgacgaaca tgacatgaca ttggttggaa taatggcaat
    13441 gatagaccca ccaagagaag aagtaatgga tgcagtaaaa gaggcaaaga gtgctggtat
    13501 aaaaactgtt atgataactg gtgaccataa aacaacagca gcagcaattg caaaagagat
    13561 tggtataatg gaagaaggag acttggctct tactggaaaa gagcttgatg ctttgagtga
    13621 agaacaacta tatgaaaaac tagaaaatat aagtgtatat gccagagtct cgccagaaaa
    13681 taaaattcgt atagtaaaag catggcaaca taagaataat ataacagcaa tgactggtga
    13741 tggtgttaat gatgcacctg cattaaaaca agcagatata ggaatcggta tgggaagtgg
    13801 tactgatgta gcaaaagatg cttctgctat ggtattggtt gatgataact ttgcaagtat
    13861 agtaaatgct gttgaagttg gtagaacagt atacagtaat ataataaaat caattactta
    13921 tctatttgca ggaaacttag gagctatagt atccatagta tttgcagtgt ttgcaggttg
    13981 gtcaaatcca tttacagctt tacaactttt gtttataaac ttagtaaatg actctttacc
    14041 agcaattgca ttagggtttg aaaagtcaga aaaaaatata atgaataaac ctcctagaca
    14101 cccggatgaa agtatactag caggaggaac attgaaggca gttgctataa gaggttgtat
    14161 aataggtgta gttacaataa ttgcacaata tataggatta caagtatctc cagaacttgg
    14221 agtagcaatg gcattttcaa cattgatatt agctagaata atgcaaacat tacctgcacg
    14281 ttctaataat caaacagtaa taaaattagg ttttttcagc aataaatacg ttataggagc
    14341 agttttagtc tgtcttgggc tttactcttt aacactaata ccagtgctaa gaggaatatt
    14401 ctctatacca acaacgtttg gattgtacca gttggggatt tgcctagggt tagcattaat
    14461 ttcatcaata ttaatggaac ttaacaaatt aataaataga agtgaagtaa aataatcaaa
    14521 aaattaaagg gcatattgat tttaacaata tgccttttat aaatagtcta atttattcta
    14581 tattatcgat tatttggaat gcttttataa cttcaggtaa ttctttttct tcaggcattc
    14641 taaatcttat gaaattttta tcaagtccag taaaatcact tccagatata actcttattt
    14701 ttaatttagc aaactcttgt tgtaaatcta tgtccttgtt tttatgttca actgtcataa
    14761 ttgatacagt atcactagtt tcagctatat taaggttttt ccatggaatt aataattgat
    14821 tttttatatc ttttgttttt tcttttaatt cttctataaa ttgaacatca tctaataaat
    14881 ttgctgcaat actacgagat atttcagaca cttcgtaagg agtagaaatc tttttaatag
    14941 ggctaacaag ttgttctgga agaactgcat atcctgctct tagtcctgct aaaccgaatc
    15001 cttttgaaaa tgtttttaaa gcaattacat tatcataatt atttagaagc ttgacagctg
    15061 aattttcttt tggcatgtat tcaccataag cttcatctac cataactgct atatcatatt
    15121 tagctgcttc ctttactata ttttcaattg atgataaatg tataatttgt cctgtaggat
    15181 tgtttggatt gtctatgtaa attactttat attctggatt tattttttca ataaattctt
    15241 tttcattaaa cttgaaatta tcctctttct ttaaaagaac ataatcataa gttgcaccat
    15301 gcatttttat atctgtctca tattctgaga actgaggtga atatcctaaa actttatctc
    15361 ccttttcaat aaatagtcta tttaaaaggt aaataacatg tatagaacca tctgccaaac
    15421 aaattctgtc agtatctaat gctataaaat ctttccaata atcgactata ttatctttta
    15481 aagcaattga atgaggatat cctcttatca tatcaaattt taaagcatta aaagcttcta
    15541 cagctttgtt tgagtaagac acggtattta taccttcacc acagtctatc tcgaatacat
    15601 cttctgaatg tccagattct attgcataac tttttttcaa ctgcatttca atagctttgt
    15661 taatttttag tttcatactt tatcactcct tatttagtca ataataaatg ttataaaagt
    15721 tgctagattt aaaatattat gaaaatatat aatagtaaaa aatgaatata aatattataa
    15781 tagctcactt tatttaatat gattatatat catatattgt aaaatttcta gcattataag
    15841 actataaatt gaaaatattc agaaaaatac tacaagaaac gatttatttt gctcttaaaa
    15901 taagttaaat gaatgttatt aattttgtaa attgaaatag tttaagtata aagttatgat
    15961 ataatctaat aagggtaatt ctttagatta tattaaggaa atataggtat acaataaaaa
    16021 gttatggagt tggattatgg gatataaatt aattgtaaca gatatggatg gaactttatt
    16081 aggcaataat cataaagtta cagatgaaaa taaaactgca ctacaaaaag ttataaaaag
    16141 cggtattaat gtaactttag caacaggaag agccttcgat tcagctaaat gcaatgtaga
    16201 ttttcttaaa gaggacatgc caataatagc ttgtaatggt tcattaataa gagagcaaaa
    16261 tggaaatata atatattcaa ataagattga tacaagaaca tgtttaaaca ttttagatgt
    16321 attagataaa tacgatattt actaccaatg taatagtata gattctatgc tttcgaaaaa
    16381 aatagaagga cgtgaagata gattaagtgt atttttaggc tctgaaacag aagtaattgt
    16441 aaaagatgat ttgagagaag aaatctttaa aaaagatata ctaaaatttg taatcataga
    16501 agaaaaaaat ccatctattt tagatgaaat aagaaaagaa ttgagaaaag ttcaaggaat
    16561 aaagataact tcttcatggc caaacaatat tgaagtcatg aatgaaggtg ttgataaagg
    16621 taatgcagtt aaaattttag ctgaaaaaat gaatatagac agagaagata taattgcatt
    16681 tggagacaat tacaatgaca tagaaatgat aaagtttgct ggtcttgggg tagctatggg
    16741 aaatgcagaa gaattgatta aacaagaagc tgattatgtt acagatacaa atcaagacag
    16801 tggagttgca aaggcaattt ataaattcct agagttaaaa gaatcactta gctcttaaaa
    16861 cagtatataa taggaaaaaa taggttgact attttgtaga tagtaattat aatacaaata
    16921 gataaaacat aaaaatttag attcagtgaa aaagagagta gatatagaga aaatctcagc
    16981 gaattggggg atggtgtgag cctaatgata acactttata ttgaaaagaa ctttggagaa
    17041 tactacttga aattcagtat ggtagtaggt aatctcgcct taagagaata gagtgggtaa
    17101 acattgttta ccaactagag tggtaacacg gaatatttcg tctcttggtc tttttgacca
    17161 agagattttt tagtttcatt gagttacaag acttataatt aaaatacaaa aaattaaaat
    17221 atgaggtgaa agttatgaaa ttatggggcg gacgctttag aaaagcggaa aatcaactta
    17281 tggaagaatt taataaatct tttggctatg attgtgtact gtacaaaaaa gatatcgaag
    17341 gaagtgtagc acatgttcat atgcaagtaa aatgtggact tttaacagaa gaagaaggaa
    17401 agtctataac agaaggttta aaagggatac ttgaagatgt agaaaatgga aagttagtat
    17461 tggatggaga gtatgaagat atacatagtt ttactgagat aaatttaatt caaagaattg
    17521 gagatgttgg caagaagctt catacagcac gtagtagaaa tgaccaagtt gcagttgata
    17581 tgagattgta tgctaaagaa aaagcaaatg atttggtggg tttaatatct gaatttaaag
    17641 ctactataaa agatgttgct gacaaaaacc cagtgatgat gccaggctat actcatcttc
    17701 aaagagctca agtagtgaca tttaagcatc acttaatggc atattatagt atgtttgata
    17761 gagatgagaa aagaataaaa aatgctatag aaatattaga tgaatcacct ttaggctgtg
    17821 gagctttagc aggaactacg catgatatag atagaagtat aacttgtgaa caattaggat
    17881 ttaaaaaagt agtagataat tttatggatg gagtaagtga tagagattat ttactagagc
    17941 taatgagtga tttttcaatt ataatgatgc acttaagtag attaagtgaa gagttgatac
    18001 tttggagttc tcaagaattt ggatttgttg agatagatga tttatataca actggaagta
    18061 gtataatgcc tcagaagaaa aatccagatg gtgcagagct tataagaggt aagactggca
    18121 gagtatatgg aaacttattt ggtcttttta cagtaatgaa aggaatacca ttagcatata
    18181 ataaagacat gcaagaagat aaagaaggtt ttttcgatag tgtacatact cttgaaatgt
    18241 gtatacaaat aatggataga atgatagcca cactaaaggt aaatgaggat aaaatgaaac
    18301 aagcagtaaa aaatggtttt ttaaatgcaa cagaagttgc ggattatttg gttaaaaata
    18361 atgttgcttt tagagatgca catggtatag taggaagtat cgttatatat tgtgaagata
    18421 ataaaaaagc aatagaagat ttaacattag aagaattaca taagttttca gatgctttta
    18481 aggaagatat ttatgatttt atcgactatg agagtatatt aaataaggga ataaaaaaga
    18541 atttaaaata atgtatttat ttttaaaagc tgtaaatttg aatgttatca tttaaattta
    18601 cagcttttta ttgtgtaaga ttttattata taaaagtaag aaacttaaaa gtagtagtat
    18661 ttaaaataaa tacaaataaa aaattgttat taaaatgata attaatatca ataagtattg
    18721 acaaaattat ttctaaatac tattatatat aatataaata ataatgaaaa tcaatatcaa
    18781 ataaatggga ggaaatctaa tgaacttatt agtgattggt ggtcatgaaa gaatggaaaa
    18841 agattatatg gttatggcca agaaaaaagg ttataagaca aaggtatata ctaccatgtc
    18901 atctaaatta aataattcta taggaagacc agatgcagtt gtcatactta catcaactgt
    18961 ttcacataag atgtctagaa cggttgagtc tcaagctaaa aaacaagata tattaatcgt
    19021 aaggcataaa aatagtagta aggtagcatt taatgaatgt ctagatatgg ttgatgaatg
    19081 tctgggaaat tgttcaaaat gtaagcttaa agacaaacaa aaacaaaaaa atatataggt
    19141 ataaaaaata cataaacata aaattaaaag tactgagata atagttataa ttattatctc
    19201 agtactttta gatttataag gataagtata ttataaattt tttattataa tttccaacta
    19261 cttgttactt tttcatattt tttttacttc tagctttttt tctttttttc ttctttatat
    19321 ttgaagctat tataaataaa actaaaagca ctagaatacc tgcaataatc ttaacaaaag
    19381 tcattaaaat attatttcca gtaaaaaatg gaagactgct atcaagatta ttttttgcaa
    19441 ttaagtctat actgttttct agtttaccat tattatatac atttaaagta ccaaccttag
    19501 cccctttttt tataggcaaa tcaattttat ttaactctgt tttggtatca taattgcctt
    19561 gagattcatc tttttctaag aatatcctat aactattttt tggttcataa atcaattcat
    19621 gttgttttgt aaacaatact tttttagtat ctgtgtattt ttctttatca acaatagtag
    19681 cacacttaaa gttatcaaat ccataatcaa tcagaatacg tgagtctaaa tataaatcag
    19741 cattagttga attaaataca gcaacgataa ctcttctgcc atcttttaca gcactcgata
    19801 aaagacattt accagcagca tcagtatatc cagtttttat accatcaact atatcatatt
    19861 ttatatttat aggttgacct ttgtatgtaa tcttatcatg tgatgtcaaa aacttattag
    19921 tgtttacaaa gtatctctca taaggatatg cttttgtagc ttcaaattta agtgacttag
    19981 tttttacaat ttgtctaaaa gtatcattat tcatagcttc tctagctatg agagccatat
    20041 catgagctgt tgtgtagtga tttgggtctg gtaatccatg aggattatta aaatgagtat
    20101 tagtagctcc aatttttttt gcctcagagt tcataaggtc aataaatttt tgtacattgc
    20161 caccaccaac atatctagct aatacaaaag cagcatcatt agcagaatga accaaaagag
    20221 catctagtaa ctgctttaca gtaaaagatt ccccattttt tagatacata ctagagccat
    20281 ctatttgagg taaatcttta atttctatta cttgatttaa atctttaacc tcctttagaa
    20341 ctaaacaagc tgtccatact ttagttgttg aagctggata aagcttagaa tttccatcct
    20401 tattatatag tattttgcca gactcatagt ccattaaaac tgcatgttta gcaactatag
    20461 ctggttcatt atctgcaaaa gagatattag agcttcctaa aaatgaagta aatatcaata
    20521 aacagattaa taaaagtgat aaatttcttt tcattgtgag cctccaaatt ttaaattcat
    20581 cttttagtat agcatatatt tgtcaataat taaaagaaag gagtgattaa tatatgaaaa
    20641 aaaaagtaaa atttatttcg ttttttgttt tatttatttt aataatatca tcaataatcg
    20701 tttataaatc gcttattttc aaagatgata tttatgttgt aagtaaaaaa aatttaccta
    20761 atgaaaaaac acatgttgta gagaatgaaa ttaaaggtga agataataat cacaataaag
    20821 taactaaaga catagataaa aattttcaaa ataataaaat aactatatac ataagtggag
    20881 cagtaaatag acctggaatt gtaactatag agtcagacaa gagactttat gatgctgtag
    20941 aattactagg aggaacgaca aaagaagcag atttaaatgg agtcaatcta tcagtaagat
    21001 tagaagatga acaacattac ataataccca aaataggtga agcaacatct gttacaagta
    21061 atgatgattc caataaacca aatcagagga atgagtcaag aggtgagcct aaaaataaat
    21121 ccaataaaaa tatatctaac gaatcaaaag taaatataaa tgtagcgact atagaagagt
    21181 tggattctct acctggggtt ggagaagcta cagccaataa aattttacaa cacagagaag
    21241 aaaatggaca gttcagttca attgaggaaa taaaaaatgt aaatggtatt ggagataaaa
    21301 agtatgaaaa tataaaagat ttaatttgtg tagactaaaa tataatacaa aacttacata
    21361 tagatatgtt attataaata tgtatgtata tgtgtgtaca tgtggaaatt tagtaaagga
    21421 ggcactttta tgaaaaaaga acaaaaaaaa cttacagaga tgacaacagc aggtggttga
    21481 gccgctaaaa tagggccaga ggttctggca tcagttttat ctcaattgcc taaaaatgat
    21541 aatatagaaa atttattagt aggactagat acagcagatg atgctgcagt ttataaatta
    21601 aatgatgata tggcacttat ccaaacttta gacttcttta caccaatggt agatgaccca
    21661 tacgtatttg gtcaaattgc tgcatcaaat tcactatcag atgtatatgc tatgggaggc
    21721 aaacctttgg ttgctatgaa tattgtttgt tttccatcat gccatgatat ggatgtactt
    21781 gcagaaatat taaaaggtgg gtttgataaa gtgaaagaaa gtggagcact tttggttgga
    21841 gggcatactg tagatgacaa agagcctaaa tatggtcttt cagtttctgg gattgtaagt
    21901 ccaaataaag ttttatcaaa tgctactgca aaacctggtg ataagttaat tataacaaaa
    21961 ccaattggag tgggtgtatt aaatacagcc atgaaagaag gtatggtaga acaacatata
    22021 gcagataaag taatagaaat aatgatacat ttaaataaat atgcagcaat gagttttgag
    22081 aaatttgatg taaattcggt tacagatata actggttttg gtctacttgg tcatacttta
    22141 gaaatggcca aagcttctga agtaagtatt gaaattgaat caaaacatgt accaattatt
    22201 gaaggtgcaa tagaaatggc acaaatggga attatacctg ctggtatgta taaaaatatg
    22261 cattatgtat ctaaagatgt agaagttgta ggaaatatag aagttgcagt tcaagatatt
    22321 ttatacgacc cacaaacatc aggagggttg ttaatttcag taaaagaaga acttgcagag
    22381 gaacttgtaa aagatatgaa attaaatgga gcaatagaag ctaaaattat aggttctgta
    22441 gttccaaaag gggaaaaata tattacagtt ttataaaata ttatagagaa agagtgattg
    22501 ttttgagcaa aagagaattg tttgcaatgt tgccttcagt tgatgaagta ttaagtgata
    22561 atagaattgt agaaataata aatgaatatc ctagaagttt agttttagaa tctgtgagag
    22621 aagtaataga tttaaaaaga caatttatat taagattaaa agaagatgca tcaaactctg
    22681 taacaataga gtttggtgaa ataatagaat ctgcaataga gagagttaag ttaaattact
    22741 cattatcttt aaaaaaagtt ataaatgcaa ctggtacagt tattcataca aatttaggaa
    22801 ggtctttact tagtgaagat ataaaagatg aattatggtg tgctgcttca aggtattcaa
    22861 acttagaata cgatttggat aatggagaaa gaggttcaag atactctcat ttaactagta
    22921 caataaaaag acttactgga gcagaagatg tactagttgt aaataataat gcagcggcag
    22981 tacttcttgt acttagcaca atggctaaag gaggagaagc aattgtatct cgtggagaat
    23041 tagttgaagt aggtggttca tttaggattc caagtataat ggctcttagt ggtgcagaat
    23101 tagttgaagt tggttcaact aataaaactc atttgaaaga ttacaaggag gccattacag
    23161 aagatacaaa tgtattgatg aaggttcata caagtaatta tagaataatg ggattcacag
    23221 agagtgtttc tatagaagaa ttggttaatt taggtaaaaa atataaacta cctgtaatag
    23281 aagacttagg aagtggagtt tttatagatt tatcaaaata tggtttatct tatgaaccaa
    23341 ctgtattgga ttctattcga caaggtgcag atgtagtaac ttttagtgga gataagatgc
    23401 ttggaggtcc gcaagcaggt attatagttg ggaaaaaaga gtatatagaa aaaatgaaga
    23461 aaaatcaact tactagagca cttagagttg ataagcttac aatatgtgct cttgaagcaa
    23521 cattgagaat gtatttagat gagacaaaag ctatagaaaa tatacctact ttaaaaatgt
    23581 tgacatataa aattgaagaa ttagaagtaa aagcaaataa actatttgaa aaaataactg
    23641 ctttaaactt aaatgcaaac ataaatatag aagatggatt ttcacaggtg ggaggagggt
    23701 ctatgcctct tgaaactata agtacaaagg taatatcaat aactcctgaa catatgaatg
    23761 tttcatcttt agaaaaaaaa ctaagactta gtgaagcaca tataatagcc agagtgtatg
    23821 ataataaata tgtactagat gtaagaacta tatttgatga tgaatttgat gtaatagttg
    23881 aggaattgag aaaagcattt aattaaataa atttgagtta ataaatatta aatttagaag
    23941 tataagtatg aagttttgat taaaaaatag ctacacacac ttgtgacttt agtcatagca
    24001 ggtttagagg gggcaaaaaa tgaaaaatgt agtaattgga acagcaggtc atattgacca
    24061 cggaaaaact actcttataa aagcattgac tggaagagag actgatacgt tagatgaaga
    24121 aaaaaagaga ggtatttcaa taaacttagg atttacttat tttgatttac caaacaatag
    24181 aagagctgga attgttgatg tcccaggaca cgaaaaattt ataaaaaaca tgttagctgg
    24241 agcatcagga attgatatag ttttacttgt tgtagcagca gatgaagggg ttatgccaca
    24301 gacagttgag catttagata ttctaagctt tttaaatatc aaaaatggaa tagtggttct
    24361 aactaagtct gatactgtag atgatgaatt tagagagcta gtaaaagaag atatttgtga
    24421 aaaaataaaa ggaacttttc ttgaagatgc tgaaataatt gaagttgatt ctatatctaa
    24481 aagaggtata gacactctta ttgaaaaaat agataaaatg agtgatgata tagaagataa
    24541 aaatgaaaac tctccagcaa gacttaatat agacagagta ttttcaataa aagggtttgg
    24601 tactgtaata acaggcactt taatagaagg aaagataagt atagaagatg atttggttat
    24661 atatccacag gtattaaaaa caaaaataag aagtatacaa gttcatggag aaaataaaga
    24721 tacagcttat gcaggtcaaa gaacagcaat caatatatca aatgtaaaag tggatgatat
    24781 aaaaagagga gatgttttag ctgcaccaaa ctcattggag gaatcaatga tgttagatgt
    24841 aaaattatca ttggtaaatc atttagataa aggattaaag cactgggata gattaagatt
    24901 gtatcatgga actagagaga tactttgtag agcagttcca ctagataaag aattaattga
    24961 aagtggagaa agtggctatg ttcagttgag acttgaagaa agcatagtat ctaagaaagg
    25021 tgatacgttt gtagtgagac gttattctcc aatggaaact ataggtggag gtgtaataat
    25081 tgacccatct cctaaaaaac ataagaaatt tgatgaaaaa gttatagaag cattaaaaat
    25141 aaaagaaaaa ggcgaactaa aagatataat agaagagtac ttaaaaagga atttaaaaaa
    25201 ttatccaaat ataaaagaaa taatgagcta tagtggtgct catgaagaag atgtaaaaag
    25261 agcgcttgaa acacttatat cagaagataa agtatttatt ataggaaata tgtatatgca
    25321 tataaatcaa tacaataaat taaaagaaaa tacaataaag cttttaagtg agtaccataa
    25381 gaagtatagg cttagaaaag ggatactaaa agaagaagta aggtctaaga tagagagtaa
    25441 ttttaagact agggagatgg atattttatt agaaaaattg tctacagaaa atgctataaa
    25501 aatagaaaat aatatagtat ctctattaga ttttgaggtt attttaaatg ataagcaaaa
    25561 agaaatagca aaaaaaatag aaaaaagatt aaaatcatgt ggagtttctt ctatactgac
    25621 aatagatgaa gtttctgaag gaaatcataa ctatgctgaa gtattggagt caatgatagg
    25681 taataaagta gagaaattag atgatttgta tataatggac aaagatattt atgaaaatgc
    25741 aaagaacatt ttgataaatt atataaaaga aaataaagaa ataacattag gagaatacag
    25801 agatttgatt gattctagca gaaagaattg catgattata ttggaaaact tcgatagaaa
    25861 caaaataact aaaagagtag aaaacaaaag aattttgttt tagcagaaaa tgtctgtttt
    25921 tgctttaagg tataaagaag gaattataat gtcgagtatc gtataattaa ttagtataaa
    25981 atattaatta agaggaagtg taaagtattg aagaatataa tacctgatgt aaaaccagat
    26041 ataaaagaaa gagaagacat gagcctttta tttataataa tgggctcagt acttttatta
    26101 tttattataa aagatataga gaaatatgaa actttattac atgccatata tacgatattt
    26161 ttagtagcta caggagtaat tatatttaat actacaaaat ttaaagtaga ttcattttct
    26221 acatttttag gattaatttt tatagctact ggagtattgg agtgtatata tttatttaat
    26281 tccttgggga ttaaaactaa gtctataatg gagattaaca tcacaatatc agcaataaca
    26341 gatttatttc ctatactggg tgtatactta agttttaaat ttgttaagga taacaaacaa
    26401 atttattcaa gtgtaatttt atttatagca actgctacat taacaataac atctttattc
    26461 ataatatcag gaatttgtga ctatctggga aatggtaaat cacaatatgc tttaggagtt
    26521 ataatatcta tattcataat aattatttct atcattagtg gaattgaatt aaattcaagt
    26581 tcaaataaag gtaaatggga atatgatgaa aaaaagattt ttaacagaat aatagtaata
    26641 ataatcttat caagagttcc aaatctattg catatagtta ttgataatag aaatgtagag
    26701 aggatattat cacaaataat aataaatgta gccttatatt atttatataa ttacatagta
    26761 tctaagaata ttaagaaaac agcactggaa ttgcatgata cgaatgaaga attaacaaaa
    26821 aaaacggaaa gtttaaaaga aaagaataaa aaattaatct atgagacaga aaagatagag
    26881 gaacttaagg gaatacttat gaaaagagaa tctaggctac aatctacatt agatgtagca
    26941 gtaaatagca tagtagtatt tagcaaagat ggagaaatta catatgcaaa taagagcttt
    27001 agaaatactt ttggagacta taaagaacaa gataaattga ctgataaaat aaaaaacttc
    27061 aatgatttga tagaaagcat tcattatgtg tttgttcatg agaagaatgc agagaaactt
    27121 attagtacaa gcgataataa agtttatcaa gctatatttg cacctttaat tattgcttca
    27181 caaacagaag gagtattgtg tgtactagaa gataagacta agaaaaagga gtacgaacgc
    27241 aaacttattg aagcaaataa gagaagtaaa gattttttag aaagtattgg agatggcata
    27301 gtagtgcttg aaggtaataa caaaatatat gttaataatg catgtaggga aatttttaaa
    27361 aataatttag agagtattga tttttcatta gtatgtaaaa gtgaagaaag tatagaaaaa
    27421 agatatgtaa tagatggtga agtaaagtat gtagagatga gtttttctca gtatactaat
    27481 gctggtacta ataaaactat tattgtaata cgtgatacaa cctctcgaaa aatatctcaa
    27541 ataaaacttg aggaaagtca atcatcatat tcaagattta tagatatatt acctgatggc
    27601 atatgcttat taaaagaaga tttaagtata aactatgcaa ataagtcgtt tttagatatg
    27661 ctagctttta caaacataga tgatataaaa gattctaata ttaaactatt aatgaatgcg
    27721 aatactgaag aaaaaatgaa atttacggat aatatggcta aagttttaaa tgaaaataga
    27781 agtatacttt tactggaata tgaattgata aatagttatg atgatatagt agaagtagaa
    27841 ttaagtgcat taccttttgc tatatacaat actagataca taatgcttat tataaaagat
    27901 ttagtacata agaaatattc cgagcaagca gaaaaagaac tgcttgaaag atttaaaaca
    27961 gataaaatca aaacggagtt ttttgcaaat atgtctcatg aactaaaaac tccactaaat
    28021 gttatatcta gttcaaatca actggtagat tctttctata gaaatgaaaa gataaaagat
    28081 tacaataaca atattaaatc tcatgtggat ttagttagac aaagttctta taggcttcaa
    28141 agattaataa ataacattat agatttaaca aagatggagt ctgggttcta tacactaaaa
    28201 cttgcgaaat acaatattgt atctgtaata gaagacttat ttatgaatat agaagagtat
    28261 gcacttagaa aagacataaa aattcttttt gatacagacc ttgaagaaat taatgtttat
    28321 atagacaagg ttgaaattga gagaattatg ttgaatctat tgtcaaactg tattaagttt
    28381 actgataatg gaggctggat atatgtaagt attcattata aaatagataa agtaatcata
    28441 agtgtaaaag acactggagt tggaatacca caagataaat tagagttgat ttttgaagaa
    28501 ttttcacaag ttgataagac tttatccaga aatactgaag gaagtggaat agggcttgct
    28561 atagttaaga atctagtaag tcttcatggt ggagatatag aagtagtaag tgaagtgaat
    28621 aaaggcacag agttcttaat ttcacttcca atgaagggat ttagtagtga acattacaca
    28681 gaagataaga gaatatataa tattcaagaa aaaataaaaa tagaattttc tgatatatat
    28741 tattaaaaat gattatgagc aaaaataaaa taaaaatact atttgtttaa atatttaagt
    28801 tgtaagaaat aggctatcat aaggtaaatt attatgatag cctattttta gctaggtttt
    28861 atatctatat gtgagaacat ataatctcta aattaccttc aaaactaaaa tctccaaagt
    28921 cgtttggtag tataaaatgc ataccctttt ttaaattgta ttctacagaa ttgtgtataa
    28981 gttttccatt tccatccaac acactgtata aagaaaaagg tgtattgtgt gtaaaattac
    29041 attttccaaa aacatctaat ttataaacac taaaaaattc gtttgacaca aatgttgtgc
    29101 atttaaagtc tgatgttgat acgattttat agtcagtatc gaaatttata tgaggaacat
    29161 tcgtaacatc tattgatttt tgaacatgaa gttcacgctt attacctgaa ttgtcagttc
    29221 tatcataatc atagactcta taagtagtat ctgagttttg ttgagtttct aaaatcaatg
    29281 ttcctttgca tatagcatgt atagtgccac ttggaacata gaaaaagtct ccctttttta
    29341 tattaacctt tctaagtagt ttgtcccatt cattattatt taccatattt acaaattctt
    29401 tatgagattt tgcattatga cctattataa tttctgcatc atcagaacaa tctattacat
    29461 accaacactc tgtcttacct aactctccat tttcattttt tttagcatat tcatcatttg
    29521 gatgaacttg aactgataaa ttatcatttg catccaatat ttttgtaagt aaaggaaact
    29581 tatcaccagg tgtgtttcca aataattctc tatttttatt ccacaattca gatagttttt
    29641 tacctttata ttttccattt tctattaagc aatctccatt tttatgagag cttatagccc
    29701 aacattcacc agttgttggc gaatctattt cataattaaa tttatcttta agtgcagtac
    29761 caccccatat tctatccata aaaataggtt ttaaaaataa tggttccata atcctgttcc
    29821 tcctatgata ttaaatttat cctacaaata attatatata aatagattat aaaatcaata
    29881 gtaaaaaagt tttgtccctt gaaatgacat aaagtataag gagttattaa ataagataaa
    29941 ctatgatata taatataaag ttctaaattg gagtgataat tatggagttg tttaaggcag
    30001 aaagcaaaaa atataaaaaa gaatttttaa aattattgga gcaatgggtt agtatacctt
    30061 cattttatga tagaaaaact gtatctaaag acatgccttt tggaaaagga gtatatgatg
    30121 cattaaactg gtttgaaaat ctaggtagag aaaacaattt caaagtaaaa aatatagata
    30181 atcatgctgt tcaaattgaa tatggaaatg gtaaagagta tgtagatata tttggacatt
    30241 gtgatgttgt aaatccaggt gaaggatggg atagtgaacc atttaaacta aatattatag
    30301 gtgataaatt agttgctaga ggtgtatctg ataataaagg gcctatgata gtgaattttt
    30361 tagcactaaa aatgataaag gatttagata ttaatttaaa aagaaaggtt agattaatag
    30421 ctgggggaaa tgaagagagt ggatttaaat gtataaagca ttattatagc aaagagccat
    30481 atggtgtatg tggatttact ccagatgcta aatttcctgt attaaatgga gaaaaaggtg
    30541 gagcaataat aaagttaatt tcaaatatag acgataaatc tttatatata agtggtggaa
    30601 tagagtttaa tacaatacca gataaagttt atataaagaa tgtagaaaaa ttgggtaaag
    30661 ataatatatg ttttgatatt aacaatatat cgataaatta tgataatggc aattatattg
    30721 tgtatggaaa aggtggtcat tcatctaaac ccgaaaaatc aataaatcct atattagcta
    30781 caattaaact tttgtcagaa aatattgatg aaaaatggac aaaagattta tataagttga
    30841 taaatcaaga taatataaat ggaaacttat ttggtttaaa tattgagggg aaatgtggaa
    30901 ttttatcaat ggtgccaaca ataataaata tagtagatgg aaaattagaa gtagttttaa
    30961 gcgttagata tccagaaata ttaaccattg aagatataat aaaaaaattt aatttgtata
    31021 tggagcaaaa taatattaat aaatttgagc ttattgggga aaatttaaag caagcaaatt
    31081 atatagatag aaattcaaaa cttgtaagaa gtttgcatga tatatatata aagtattctg
    31141 gagatttaaa aaatgatgtt agagtaacta gtgcaggaag ttatgcttct gaaatgaata
    31201 attcagtaat atttggttgt gaatttcctg atggtagctt tggcaatgtc catagtgcga
    31261 atgaatttgc tagcttagat agatttataa ctgcaatagg tatatatgca gaggcaataa
    31321 ttacattatg taataaaata taaatacaaa atgtccctta tatagacata ttatatatac
    31381 aattaaaatt ttacttgata tataattgaa atcaaagaga aggtgataca atgataacta
    31441 agaaacatgc aataatagta aagagtttaa gcaataaaga tggatatatg acatcaaacg
    31501 aattagctat caaattggat gtgtctacta aaactattaa aagatatata gcagatttaa
    31561 atagtgtttt aagcaaatat gatttggtaa tagcttcttc tagaggtata ggatacaagt
    31621 taagcggttc taaaaacaat atagccagag ctgttaaaga agcaaataaa tatattgatg
    31681 gttttttaga tgactctgaa gagtcaagaa tgtcaaatat aatatgtatg cttattaata
    31741 gaaattatat gagtatagaa gcaatggcag aagaattaaa tctaagtata gccgctataa
    31801 ataaattgtc tagtaagctt aaaagaaaac ttgaaaagta tgacttagtt ataaaatcta
    31861 aaccttacta tggaagctat atacatggtg aagaaattaa tataaggcaa ttaattactg
    31921 actatgcaat aaaattagat gaaaaaagta aagtgaaggt gtttttagat gacattagtg
    31981 aaaacgatat acactgtata gaaagtattt tagaaaagca cttaagagaa aaagatacaa
    32041 taatttcaga taaagatttt aatcttttgc tttcaaaaat aatcgtttct gtatttagga
    32101 gtaagagagg tcatagtaac aatataaact taatggatac aagttataga tttcataact
    32161 actactttat agaaaatcta atgaaagaaa tatctgataa aattggattt aaacttattg
    32221 aagatgaagt aatttatatt tcaaattatt ctggtgtaat agcctataaa ggtacacaag
    32281 gaagaaaaaa cacaagtgaa attgaggaac gaataagtac tgttataagt agtgcacttc
    32341 aagatatatt ccttatttca ggtagtgatt atacaaaaga tgatgagttt atggttgcaa
    32401 tctctgacca tataaagaga tttttaaata gagctagagc taatgtaaaa tcaaataatc
    32461 ctttacttca tcaaataaaa gagaagtttc ctatagcttt taatttagcc gtgtttttat
    32521 caaataaact agaaactgag tttaatttaa aacttgatga agatgagctt ggatatatag
    32581 ctatccactt tgctgcttca aatgaaagaa tgaaaaaaaa tactagtaag aaaatttgta
    32641 tagtttgtca ttatgggata ggaacaggac aacttatttc tgaaaagcta aaacagaata
    32701 taagtgattt aagtgttgtg ggtgtttatc cagttagata tctcgatatg gctataagtc
    32761 aagatgttga tttgatagta tctacagttg aattaaaagg atatgaaaaa cctgtcttat
    32821 atatagaaaa tatatttgat gatagcttaa ttgaaaacgt aaacaaagcc ttttatgaaa
    32881 aggaagaaag aagaaagata ataagtaaca tgtttgatga aaaagctttt ttcagtataa
    32941 aggctagtac taaagaagag gtcataattt cactttcaaa taaactaaaa gaaagagatt
    33001 ttatagagga aagttctata aaaagtataa tagatagaga aaatatttca tcaactgaaa
    33061 taggaaatct agtagcaata ccccacacaa tagtgaaggg agataaaaaa tctataatag
    33121 gagttggaat acttgaaaat ccaataattt gggataagca agaggttcag ctagtattta
    33181 tggtattttt taatactaaa gaaaaacata atttctcgat atttaaatac ctttataatt
    33241 ttataaaaga tgaaggagga gtaagaggaa ttattaaaat atgtgacttt aataaattaa
    33301 tggcacttat aggtaattaa ggaggaaatt aataatggga acaaacatat ttaacaaaga
    33361 atatgtattt ttaaatgttg atgctaagtc aaaggttgaa gttttaaaat ttatatcaaa
    33421 aaaagctaaa gacttaaact tggcagaaga tgagagctta gtatatgaag gtttaatggc
    33481 tagagaagaa caatttacta caaatttagg agaatctata gcaatacctc acactaaaaa
    33541 tgatgcaatt gaaaatccag cagtagtagt attgaagttt aatgaagatg ttgtttggaa
    33601 tgaaggtgag gataaggtta aattagcaat aagcttactt atgccaggaa aaagtaaaga
    33661 gaatattcat cttaaattat tatcatctct ttcaagaaaa ttaataaata aagaatttaa
    33721 agattctctt ttaaaaagtg ataatgttga agaaatatct aacttaataa atgaagcttt
    33781 aggtttataa ttaaacacta aggttataaa ataaaaaaat aattaactaa aaataggagg
    33841 aatttgaaat gaataaaaaa ttagtagcat tatgtgcttg tccaatggga ctagctcaca
    33901 ctttcatggc agcagaagca atagagcaag cagcaaaggc attaggttat gaagcaaaag
    33961 ttgaaactca aggtgctgat ggtgtacaaa atgagttaac aagagatgat atattaggag
    34021 caactatgat aatacatgca gtagcaataa caccagaagg tatggaaaga tttgatggtt
    34081 gtgaagttta tgaagttgaa ttacaagaag ctataaaaaa tgcagaaggt gttataaaag
    34141 aaatagaaga agatttagga atataataaa tttattgtgg aggaaataga atggcaatta
    34201 agaaaaaagt tataaacagt tcaggtgctt ctacaacagg aggaaataat gtaggaaaat
    34261 ctactccatc tagtccaact caatcaaagg gaaatggaaa atggaaagaa ataagtaaac
    34321 atataatgac aggtatatca tatatgatac cagttttagt aatgggtgga cttatagggg
    34381 ccttatctca attaatacca tatgctatat taggacttga cccatctgta ggtatagttg
    34441 atgctatgaa ctcaggagaa tttacagggt tcaagcttag tcttttaaat atagcacaat
    34501 taatgtcaaa tttcggattt actttgtttg gatttgcgat accactgttt gcagcatttt
    34561 gtgcaaactc tataggtgga aaaacagctt taattgcagg atttataggt ggatatatag
    34621 ctaataaacc tgtaggtgta ccacaattcg ttgatggaca atggactgaa gttgttccag
    34681 ttgcatcagg atttttaggt gcaatactaa tagcatttat aataggatat tttgtaaaat
    34741 ggttaaataa atctataaaa gtttctcata actggttagc ttttaaaaca acatttttaa
    34801 taccattaat agcgtcatta gcttgtatgg tattgatgat atttataata actccatttg
    34861 gtggattaat taatgaaagt atgaaaaact tcttaacagc agcaggagca gctggagaat
    34921 atgtatatgc aacagctttg gcagcagcta cagcattcga cttaggagga ccaataaata
    34981 aagcagcagg attcgttgca cttggtttaa ctacagagaa tgtacttcca ataacagcaa
    35041 gaacaatagc tatagttgtt cctccaattg gattaggatt aacaacatta ttagataaaa
    35101 gattagtagg aagaagagta tatgatagac aattctatca agcaggaaaa acttctatat
    35161 tcttatcatt tatgggtata tctgaaggag caataccatt tgcacttgag agaccaggtt
    35221 ttgttatacc tttaaacata gttggaagtg taataggagc tattacaggt ataattctag
    35281 gagcaattca atggttccca gagtcagcta tatgggcatg gcctctagta gataacttat
    35341 ttggatatat aataggtata gcagttggtg ctatattcat agcagtagga aatatattct
    35401 atagaaataa attaataaaa gatggtaaac ttgttgtaga ttatatcgac tagtatttaa
    35461 tttttactat ctataaataa ctttggctgg taatatggtt ttaatcaata ttaccagcct
    35521 tacctatatg attaattaaa ggcggtggag ataagatatg gataagatag aaaaagtaag
    35581 agagtatttt agagaatatg atatagatgg atttcttata aatagtccta caaacaagtt
    35641 ttatgttgga aatttattta gctcttcagg ttatgttttt ataactaaag aatcacaata
    35701 tataatagta gattttagat attttgaaga aattaaaaga aaatctagtt tgtttaatgt
    35761 agtattaatg gacaaaacta gaactcattt tgatataatt aatgatattt gtagagagca
    35821 gaatataaag gaaattggat ttgagggaaa tgaagtatct tttgatttat atagaagtat
    35881 gagtaataag ttaagtgcaa ccttaaaatc agttgattta tctacattaa gggaaacaaa
    35941 aaatgaagac gaaataaaat atataaaaaa agcttgtgaa atagttgatg ctacatttta
    36001 tcatatagta gactttataa aggttggtat gactgaaaag caagttgaaa atgaaattgt
    36061 tagaattata aaagatttag gtggtcaaaa agaatctttc gatacaatag ttgcatctgg
    36121 gcttagagga gcattacctc atggaaaagc aagtgaaaaa gttatagagt atggagattt
    36181 tgttaccttt gactttggtg ctaagtataa taattattgt tcagatatta ccagaaccat
    36241 ttgtatgggt actataaata aagagttaga agaaatatat aatatagtaa gaaaagctaa
    36301 tgaagagtgt ataagggttt taagaccggg aatgactact ggagagatag ataaagttgc
    36361 aagagacata attggttcat atggatatgc caataatttt ggtcataatc tgggtcatgg
    36421 tgtaggaatt atggttcatg aatatcctgc attagctcct gaatcaaatg aagtattaaa
    36481 agaaggaatg attgtaacta tagagcctgg tatatatgtt ccaagtcttg gaggcgtaag
    36541 aatagaagat gatgtactta taacacaaga tggatgtatg agattgacta catctactaa
    36601 ggatttaata gttgtaagct aaaaataaat tagataaata ttttataaca ataagaaaag
    36661 aagaggtaaa tttaaatgta taattttgat gataaaccag atagagtaag tgaaaagtgt
    36721 agaaaatggg atttaaatat tataagagat aaatttggag atattagaga agattttata
    36781 ccaatgtgga ttgctgatat ggattttaaa ataccaacag aaatagaaaa caaatttatt
    36841 gaagctgtaa gaagaggtgt atttggatat acatactgtt atgatgaatt ttatgattca
    36901 gttattggtt ggcaaaaaaa tatgcatgag gtaaatgtag aaaaagaatg gattacatta
    36961 acttatggaa ctgtatcaac acttcattat gtagtacaag ctttttgtaa gaaaggtgat
    37021 agtataatat taaatacacc agtttatgac ccctttgaat catcagcaaa aaaacaaggt
    37081 gtaaatgtaa tttgtaatac attagatgtt gtaaatgata gatattatat aaattttgat
    37141 aaattagaag ttcaaattaa agaaaataag cctaaattaa tgatgttttg tacaccacat
    37201 aatccatctg gaaggatatg gacaattgaa gaaatgacaa gggtagcaac tatatgtaaa
    37261 gaaaataatg ttattttggt tgcagatgaa gtacatgcag agcatattca ttatggtaca
    37321 tttaattcaa tacttaagat tggaaaagag cttttagaaa atgttattct attgacatca
    37381 ccaaataaag gatttaatct tggtggatta aagacttctt attcaatagt tataaataag
    37441 gatataaggg acaaatttag aaataaatta aagcaaaatt ctataacttc tccaaatgtg
    37501 tttggaataa taggattgat aaccgcttat aatgaatgtc atgagtggtt aagtggtgta
    37561 aatgaatata taaaatcaaa ttatgaactt ttagaaactt gggtaaataa atataataag
    37621 ataaaattga tgaaaatgga atcatcttac ttagcttgga tggatataag tggattaggt
    37681 attagtgcaa gtgagtttac agataaattg gctatagata caggagtatt attagaagat
    37741 ggaagtcatt ttgtaaaaga tggtgagaag tatgttagaa taaatttagg aactcaaaag
    37801 gaaaatgtaa ttgaagcctt aaatagaatg gatttatttt taaagtcttt ataaaaattt
    37861 ggttgaatta gtattttata taaatttaaa ctattaatat ggtatataca taagtagaat
    37921 ttgttttaga ttacttaagt atataccata ttttatataa aaattaaatt tgcattagtc
    37981 atgtgataac tatttaaatt catatgtatt ttgtat
//
LOCUS       DAMJVI010000029        37944 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.030, whole genome shotgun sequence.
ACCESSION   DAMJVI010000029 DAMJVI010000000
VERSION     DAMJVI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37944
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.030"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            302..1846
                     /locus_tag="PC545_001551"
     CDS             302..1846
                     /locus_tag="PC545_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2456323.1"
                     /translation="MRNLQKKLMFFLFICIILQITYTTPVYSLCKLYKNNSINNFESI
                     VSNNQLISKHAIQIILIMAIICVILIFYIIYDKLNFKIKLQRIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALLYIDIDKFKYINDLFGYEVGNEILRNLTKIIKRNIFEEEM
                     FARISADNFIIIMKYIEEEDITKRLKTIFEELDLFNNNQEEKYKLVLSCGIYFILPED
                     RDINSIIDRANIPHKMAKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIY
                     LQPKIELNTGEIQGSEALVRWKRPDKGLIPPNEFIPFFERNGFVINLDLYVLEEVCIY
                     LRKWIDAGINPVTVSVNVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDN
                     FDILIDIMNNLKKIGFLISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSK
                     IIVTKVIEMAKELGMKVISEGVETEEQFKLLKEVKCDMAQGYLFGKPMPIEEFEHLIV
                     SNLVRG"
     gene            2022..2390
                     /locus_tag="PC545_001552"
     CDS             2022..2390
                     /locus_tag="PC545_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456324.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEMLKLITENLKGEL"
     gene            2392..3117
                     /locus_tag="PC545_001553"
     CDS             2392..3117
                     /locus_tag="PC545_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456325.1"
                     /translation="MDKNISNVSQDGIVEFKGVNKSYGTKNVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIDTKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEIIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            3111..3875
                     /locus_tag="PC545_001554"
     CDS             3111..3875
                     /locus_tag="PC545_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456326.1"
                     /translation="MLGKLLKYELKASGRIFIPLYIAILIVAVFNGIFMNTNILQVQG
                     IGILVLTSLFMALGVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVLSFIVAVFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILLLVVVMFISYS
                     SFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPNEDTNMVYH
                     LYQSPSFMLAILGSLVVAIALFFATKFILDKKLNLE"
     gene            4097..5347
                     /locus_tag="PC545_001555"
     CDS             4097..5347
                     /locus_tag="PC545_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456327.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLANELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKIQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNIDTEPYEKSEKVNIVDGIKSVIK
                     NKSTWYNSMIMFSFVGLTSAFISLWGVRYIMDVYGVSKSFSAFIVSFFTYGFIFGSII
                     MDFVFAKIRSSKFNIIKFGAMIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNIVDGYKKGFVV
                     FIVLSIITIVSSHIGVKNDDFKAI"
     gene            5540..6424
                     /locus_tag="PC545_001556"
     CDS             5540..6424
                     /locus_tag="PC545_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2456328.1"
                     /translation="MDTTKIDDISKLDLNNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYVEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFATRVWCKTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            6783..7400
                     /locus_tag="PC545_001557"
     CDS             6783..7400
                     /locus_tag="PC545_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2456329.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            7508..10039
                     /locus_tag="PC545_001558"
     CDS             7508..10039
                     /locus_tag="PC545_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2456330.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            10473..11174
                     /locus_tag="PC545_001559"
     CDS             10473..11174
                     /locus_tag="PC545_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2456331.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            11224..12306
                     /locus_tag="PC545_001560"
     CDS             11224..12306
                     /locus_tag="PC545_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2456332.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            12567..13667
                     /gene="vanG"
                     /locus_tag="PC545_001561"
     CDS             12567..13667
                     /gene="vanG"
                     /locus_tag="PC545_001561"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2456333.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            13664..14470
                     /locus_tag="PC545_001562"
     CDS             13664..14470
                     /locus_tag="PC545_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2456334.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            14488..16626
                     /gene="vanT"
                     /locus_tag="PC545_001563"
     CDS             14488..16626
                     /gene="vanT"
                     /locus_tag="PC545_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2456335.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            16940..17560
                     /locus_tag="PC545_001564"
     CDS             16940..17560
                     /locus_tag="PC545_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2456336.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(17726..18262)
                     /locus_tag="PC545_001565"
     CDS             complement(17726..18262)
                     /locus_tag="PC545_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2456337.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(18375..19049)
                     /locus_tag="PC545_001566"
     CDS             complement(18375..19049)
                     /locus_tag="PC545_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2456338.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            19252..21126
                     /gene="asnB"
                     /locus_tag="PC545_001567"
     CDS             19252..21126
                     /gene="asnB"
                     /locus_tag="PC545_001567"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2456339.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(21218..22654)
                     /locus_tag="PC545_001568"
     CDS             complement(21218..22654)
                     /locus_tag="PC545_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2456340.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            22983..23561
                     /locus_tag="PC545_001569"
     CDS             22983..23561
                     /locus_tag="PC545_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2456341.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            23600..24178
                     /locus_tag="PC545_001570"
     CDS             23600..24178
                     /locus_tag="PC545_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2456342.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            24264..24893
                     /locus_tag="PC545_001571"
     CDS             24264..24893
                     /locus_tag="PC545_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2456343.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            24883..27642
                     /locus_tag="PC545_001572"
     CDS             24883..27642
                     /locus_tag="PC545_001572"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2456344.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            27729..29291
                     /locus_tag="PC545_001573"
     CDS             27729..29291
                     /locus_tag="PC545_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2456345.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            29292..30428
                     /locus_tag="PC545_001574"
     CDS             29292..30428
                     /locus_tag="PC545_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2456346.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            30492..31568
                     /locus_tag="PC545_001575"
     CDS             30492..31568
                     /locus_tag="PC545_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2456347.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            31546..32784
                     /locus_tag="PC545_001576"
     CDS             31546..32784
                     /locus_tag="PC545_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2456348.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            32838..33926
                     /locus_tag="PC545_001577"
     CDS             32838..33926
                     /locus_tag="PC545_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2456349.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            33954..34745
                     /locus_tag="PC545_001578"
     CDS             33954..34745
                     /locus_tag="PC545_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2456350.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(34860..35414)
                     /locus_tag="PC545_001579"
     CDS             complement(34860..35414)
                     /locus_tag="PC545_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2456351.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            35610..36812
                     /locus_tag="PC545_001580"
     CDS             35610..36812
                     /locus_tag="PC545_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2456352.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            36869..37849
                     /locus_tag="PC545_001581"
     CDS             36869..37849
                     /locus_tag="PC545_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2456353.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
BASE COUNT        14708 a         3946 c         6564 g        12726 t
ORIGIN      
        1 gaaaagaaaa tattaaaatt ataatataaa aataaaattt ttcagacaat ttaaacaaaa
       61 ataatcacct tttaataaat aattatatgc caaaataatc caatttattt tctattatac
      121 ctatatgaaa tatataaatc aatagttaag acaatgaata tactacattc tataattata
      181 gtagcagaaa attttacaaa attaaagtag atataaacta tccattttac aagaaatgga
      241 aacttttgag atgtacttat ttgctaataa aatagaggtg ttttaatggg aggaaatttt
      301 tatgaggaat ttacaaaaaa aattgatgtt ttttttattt atttgtataa ttttacaaat
      361 tacatacaca acaccagtct attctttatg caaactctat aaaaacaact ccataaataa
      421 ctttgagtct attgtgtcta ataatcagct tattagcaaa catgcaatac aaataatact
      481 tattatggct ataatatgtg taatacttat attctatata atatatgata aattaaattt
      541 taaaattaaa ttgcaaagga tagcatatac agataatctt acaggagcaa atactataga
      601 taaatttgta attgatgcta acaaaatact atgtaaaaat actcaagtaa agtatgcttt
      661 attatatata gatattgata aatttaaata tataaatgat ttatttggat atgaagttgg
      721 gaatgaaatt ttgcgtaatt taacaaagat tattaagagg aatatatttg aagaggaaat
      781 gtttgcaaga atatctgcgg ataattttat tataattatg aaatatatag aagaagaaga
      841 tataacaaag aggttaaaaa ctatatttga agaattagac ttgtttaata ataatcaaga
      901 agaaaaatat aagttagttt taagttgtgg aatctatttt atacttccag aagatagaga
      961 tatcaactca atcatagata gggctaatat acctcataaa atggcaaagg gaggacataa
     1021 aagttcatat gcattttatg ataataagat tcatgaccaa gagataaaag aaaaagagat
     1081 ggaaaataca atgttttcaa gtctagaaaa taaagaattt attatatact tacaacctaa
     1141 gatagaactt aatacaggag agatacaagg ttcagaagca cttgttaggt ggaaacgtcc
     1201 tgataaaggg ctaatacctc caaatgaatt tatcccattt tttgaaagaa acggttttgt
     1261 tataaattta gatttatatg tgcttgaaga agtatgtata taccttagaa aatggattga
     1321 tgcaggaata aatccagtta ctgtttcagt taatgtttca aggatacatt tatattgcaa
     1381 taattttata gaaacataca aaaatataat tgataaatac aatatacctg caaagtatat
     1441 agaacttgaa ttaacggaga gtatcatatt tgataatttt gatattttaa ttgatataat
     1501 gaataattta aaaaaaatag gatttttaat atctatggat gattttggct cagggtactc
     1561 atcattaaat atgttaaaag aaattcctat ggatatctta aaacttgacc aaaaatttat
     1621 tatggaaact tataatagta aaagaagcaa aataattgtt actaaagtaa tagaaatggc
     1681 aaaagagttg ggtatgaaag tcatatcaga aggagttgag acagaagaac agtttaaact
     1741 tttaaaagaa gtaaagtgtg atatggcaca aggatatttg tttggaaagc caatgccaat
     1801 tgaagaattt gaacatttaa ttgtatctaa cttggtaaga gggtaattaa tctgtaatat
     1861 aaagaattta ataaattgtg ttaaaaatgg actctaatgt agtgttcatt ttttttgtaa
     1921 aattttttat acttttacta aaaagtaatt gacatatact ttgtattatt atattattgt
     1981 attaacaact taatacaata atataataag gaggcatgaa catggaatgg gaacttgata
     2041 ataacaaacc tatatatata caattagtag aacatttaaa gttaaaaata atttcagggg
     2101 aaattaaaat aggatctaaa ttagaaacag tgagagcact agcagaagat gcggaagtta
     2161 atccaaatac aatgcaaaag gctctaacag aacttgaaag acaaggttta gtttatagtc
     2221 agagaactaa aggaagattt gtaacagatg ataaggaaaa aataaaagct atgaaagagg
     2281 aaatagctaa tgtggagata aatacattaa aagtaacctt agaaaagctt ggatatgata
     2341 gagatgaaat gctaaaactt attacagaaa atctgaaagg agaattataa aatggataaa
     2401 aatataagca atgtttctca agatggaata gttgaattta aaggtgtaaa caagagttat
     2461 ggaactaaga atgttttaaa aaatattgat ttaaatatac caaagggaaa aatagtcggg
     2521 ctgttaggac ctaatggaag tggcaaaagt acaatgatta agctaatgaa tggattatta
     2581 cagcctgata atggtgaaat tatgataaat ggaatgaaac catccataga tactaaaaaa
     2641 atagtttcat atttaccaga aagaacttat ttaaatgatt ggatgaaggt atcagattta
     2701 cttaagtttt tctatgattt ttatagtgat tttgatgtta gaagagcaaa tgagatgatt
     2761 aaaagcttag atattgatgt aaatgaaaag ctaaagacaa tgtctaaagg aactaaagaa
     2821 aaagttcaac ttattttagt aatgtcaaga aatgcgagca tatatatatt ggatgaacct
     2881 ataggtggtg ttgacccagc tgcaaggtca tatatactta agactatatt aaaaaattat
     2941 tctgaagata gtactttact gattgctacc catttaataa gtgaaataga aaacatatgt
     3001 gatgagataa tattcatatc aaaaggagaa atagtgttac aaggagacgt tgaagcaata
     3061 agagaagaaa aaggaaaatc tatagatgca ttatttaggg aggaatttaa atgttaggta
     3121 aattattaaa atatgagtta aaagcaagtg gaagaatatt tataccgtta tatatagcca
     3181 tattgatagt agctgtattt aacggaatat ttatgaatac aaatatactt caggtacagg
     3241 gaataggaat actagtatta acaagtcttt ttatggcatt aggagtttta actattgtag
     3301 ttacaattca aaggtttaga aaaaatcttt tgggggatga aggttattta atgtttactc
     3361 ttcctgttag cactagttca cttatattat ctaaatgtat aacagcactt atatacgctg
     3421 tacttagttt tatagttgca gtattcactt ttggagttct tatgcttttt ggtacttctg
     3481 gaattttact acctgaaatt ttagatttgt ttaacactag ttttaaatgg atttctgaaa
     3541 actttttaga tattttatta ttagtagtag taatgtttat ttcatattca tcatttattt
     3601 tgttattata tacatcaatc tcaatgggac aattaccaaa attcaataaa catagaaaca
     3661 tagtggcatt tgcttctttt atagctataa atatagtaat atcaattgtt ggagatgcag
     3721 ttggaagcat cttaccaaat gaagatacaa atatggtata tcatctttat cagagtccta
     3781 gttttatgct tgcaatactt ggtagtcttg tagttgcaat agcgttattt ttcgctacaa
     3841 aatttatatt agataaaaaa ttaaatttag aataaatgtt tttatatgga gccatctttt
     3901 gagtaatgtt aaaagatggc tcttattatt tatgtagtta attcatgtat ataatatgaa
     3961 tagtgtttta tttctagtta aatatataca tatgttgtat aatgatattg cgtgtaaaaa
     4021 tttgtaagaa taactataca ctttatataa gttatatcaa gaaaatttaa aaatatatgg
     4081 atttgaggga gaagatatga aaagttatac aaaggctaag tggagtgttt ggggtataat
     4141 tacattttca tttgttttag tattattttt aaggatgtca actgctgttg tttcagacaa
     4201 tttagcaaat gagttaggtt ttaattctat acaaatttca aatatagcat ccttttgcct
     4261 atatgcatat gcatttatgc aaatacctgc aggaattttg attgataaat atggagcaag
     4321 aaaaattagc agtcttggaa taataatggc aagtttaggg tcaattttat ttggtttgat
     4381 tcaaagtata gaattagcat atatttctcg tgtaattgta ggggcaggaa catcagtaat
     4441 attactttgt attttaaaaa ttcaagggag atggtttaat aagtcagaat ttgcttctgc
     4501 aacagctaaa ttttcatttg taggaaattt aggtggagtt cttgctactt ttccacttgt
     4561 gtttttgtct gaacttgttg gttggagaaa ttcattttta ttaatcggta taattggtgt
     4621 tgtaattgga tgttttatgt atattattgt tagagataca ccaaaagagt atggattcaa
     4681 tatagatact gaaccctatg aaaagtcaga gaaggttaat attgttgatg gtattaagtc
     4741 tgttataaag aacaaatcaa catggtataa ttcaatgata atgttctctt ttgtgggtct
     4801 tacaagtgca tttataagct tgtggggagt aagatacata atggacgttt atggtgtgag
     4861 taagagtttt tctgcgttta tagtatcatt ttttacatat ggatttatat ttggttcaat
     4921 aattatggat tttgtatttg ctaaaataag gtctagcaaa tttaatataa taaaatttgg
     4981 ggcaatgata gatttgttta tatggattgt aattgtagtt gtataccagg taaaaccacc
     5041 aattatagtt ttaccaatat cattctttat tatgggatgt atagttatgt cacatctaca
     5101 ggtttttaat gatgccaagt ataaaaataa agaaatttat tctggactgg caactagtgt
     5161 tattaatact tttgaattta taggaagtgg aattattaat ttgattatag ctatctcttt
     5221 acaagtaaat tcatataata tagttgatgg atataaaaaa ggttttgtag tatttatagt
     5281 actaagtatt ataactatag tatcatctca tataggggta aaaaatgatg attttaaggc
     5341 tatatagtgt tttattaata gtttaattta gtattatatt ttgcatatat atataacaaa
     5401 tagtttgcat tttcctaaga tgtataattg gaatataaat cttgtttact atgtacaaat
     5461 ggtattgtat tatattaaat agaaaataag aactttaaac tataatattg gttctaaaat
     5521 ataaggtaag gagtttttta tggatacgac gaaaatagat gatataagca agttagattt
     5581 aaataattta gatattaata agatggatat gggaggtata atggaaagtc tattagaatg
     5641 ggctactaca agcggagtaa aacttataat aggcatactt atactttcta ttggatttaa
     5701 aataataaaa aaatttgtaa accatgttat gttattactt agtaaaagag atattgattt
     5761 aactttaaga aggtttttga agtctcttct tttgagtgta cttaaagttg ccgttataat
     5821 cattgttctg gaatattggg gaatgagttt gtccagtttt gctgctgtga tagcatcagc
     5881 aggggtagca ataggtttgg ctttacaagg tagtctatca aattttgctg gaggatttat
     5941 tatactattg attagacctt ttaaagtagg agattacgta gaagcagcag gccatggagg
     6001 aacggttgaa caaataggac ttttttatac acaattggtt actccagata ataaacagat
     6061 acttatacct aatggttctg tatcaaatga tagtctaata aactattcag caaagaatac
     6121 tagacgagtt gatttgactt ttagtgtagg gtatgaagat gatatactac atgttagaag
     6181 agtacttaaa gatattgtta ataatcataa attaataata aatgaaccag aaccatttat
     6241 aggagtagta gaacatggag ataatgctat taaatttgca actagagttt ggtgcaagac
     6301 agaagattat tggacaattt atcttgattt actagaagag gttaaagtta ggtttgacga
     6361 agaaggaatt acgattccat atcctaaaat ggatttaacg gtaaaagaat taaataaaat
     6421 ttaatttttt tatgtagtgt ttaaatttaa gaatattata tagatatatt tataagattt
     6481 gtgaaaaatt aataagtcct gttattttgt aagtgaaaat ataaaaaatg ggagtgtctc
     6541 aaaatgaact ttttagttca tgaggcactc tttttgtatg aaatcaaata tatttttatt
     6601 atttcaacaa taaaaaagag gtctgtttct attttgagac aatattattt ttatgtagat
     6661 ttaatcttaa tgagtataaa gttttaaaac tactaaaata ggaggaattt taatataaaa
     6721 gggtaaataa agggtttaga gggcataata tagaataaat taattattta aggagggttt
     6781 ttatgtgcaa aaaaatgtat ttattagtgg taggtatact tatagcaggt ttattaactg
     6841 catgtaatgg gagtccaact aaggattcag aacaagcaca agataataca aaaaaggagg
     6901 caaatgtatc aaataacgct ttaaaagatg aaaaaaataa tgaaaattta atggaacaag
     6961 attttaaagt gccatacaca gatgcaataa acatatttaa agacaaatac aaagatgctg
     7021 atatagtaga tttaagtctt gagagagatt taaataaatt tgtttatact gtggaaggtg
     7081 tagatgataa taatgaatat aagatgaaaa ttgatgctaa tacaaaagat gtattagaag
     7141 ataaaacaga aaagttggat tcagaagatt taaatggagt cgctagaaaa gaaaaattag
     7201 attttaatga cataatgaca cctcaacaag ctatggaaat tgcattaaaa gaacaaaatg
     7261 gaatggttaa agagtggagt ttagataaag atttagatgt tactttttat aaaataagaa
     7321 tagataaaga taaaaatgaa tatgacataa aagtagattc taaaaaggga actgtattga
     7381 aggtagaaaa agaagattag aagatttaaa ctatctttaa aataatttct tttttgaata
     7441 aaatttgttt aaaatataac aagtagggta taaagagact aaataactat tttaggagga
     7501 gtacggtatg tcattgaaat cgctaaaaat aaaagaaaat ttatattggg taggttcgct
     7561 tgaccctgat ttaagagtat ttgacataat tatgtataca ccttatggaa ctacttataa
     7621 ttcatatgta ttaaaaggaa cagaaaaaac agttttattt gagacagtta aagataagca
     7681 ttttgacaat tatattgaaa gattaaatga tttaaatata gattttgaaa aaatagacta
     7741 tattgttgta agtcatactg aaccagacca tgcaggaagc gttgaaaagc ttttggattt
     7801 agctaaaaat gcaaaagtag tagcttcaga aacagcaatt aaatatctta aggaaatcgt
     7861 aaacaaagac tttgaatatg ttgcagttac agatggagat acattatcaa ttggggacaa
     7921 aactttagaa tttttctctg tacctatgct tcattggcca gataccatat acacatatat
     7981 aaaagaggat aaaactcttg taacatgtga ttcatttggt agtcactata gtaatgataa
     8041 gatagtaaat acacttgatg aagatgaaga aaaggattac ttggatgctt tgagatacta
     8101 ttatgattgt ataatgggac catttaaacc atctatggta actgccatag aaaagataaa
     8161 agatttagat atagatactg tatgtccagg tcatggtcca gtattaactg aaaatcctag
     8221 aaaaattata gacctttatt acaactggag tgtaaatgaa caaataaagt tagaaaaaga
     8281 agttacaatt tgttatgttt ctgcacatgg atatacaaag attatggcag aagctataaa
     8341 agcatatatt gaaaaaaata gcaactataa agtaaattta tttgatgtaa tagagcataa
     8401 gcaagaagat attttggcaa aaatagcagt atcacaaggt gtattatttg gaacaccaac
     8461 aatattagga gatgcgttaa agcctatatg ggatatatta atatcgttaa acccagttct
     8521 tcatggtgga aaagttgctt cagtatttgg ttcatatgga tggagtggag aaggtataga
     8581 aaatgctatg gagagaataa gtcagcttag aatgacagca gtaaaacctt ttgctgttaa
     8641 ctttaaacca tcaaatgaag agattgataa attacattct tatacaggta aattcctaga
     8701 taagttaaat tctacttttg gaagtaaaaa gaaaactaag aaatttaaat gtgtaatttg
     8761 taatgaagtt tttgaaggtg atagtgctcc tagtgtatgc cctgtatgtg gagctaagga
     8821 agaccaattt atagaagttg aagaagatga agttactttt agaaaagata ctgatgaata
     8881 tttcgtaata gtaggaaatg gagcagctgg tttttatgca gcagatgcta taagaaaaag
     8941 aaataaaact tgtaagataa ctatgatttc taatgaagat gagttgactt actatagacc
     9001 tgcactttct gatggtataa atgaagaact tggttcagat ttctacatgg aagataaaga
     9061 ttggtatgat aaaaataata tagtagtaat attaggaacg aatgtagata aattagatga
     9121 agtgaataaa actattatag tgaatgatgg agctattaaa tttgataagc ttgtaatagc
     9181 tacaggaagt agaaacttta taccaccaat taagggtcat gatttagaaa atgtgtttac
     9241 tcttagaaat ataaaagatt tatatagtgt aaaagaagca ctagaaaaat ctaagaaagt
     9301 tgtagtgatt ggtggcggtt tattgggact tgaagctgca tgggaattta gactaaaagg
     9361 attagaagta gtagtagttg aagccatgga tagcatacta tcaaaacaac ttgataaaga
     9421 aggcagtaaa atattagaac aatgtgtaag agatacagga atagatgtaa gattgggtgt
     9481 agcagttgat ggtattgaag gtgatggaaa agctcaaaaa gttgtattta aagatggaga
     9541 tagtgttgac tgtgatatgg tagtattttc aattggtgta agagcaaaca ctcaaatggt
     9601 tcaggataca tctgtaaaaa ttgacagagg tattgttgta gataagacat tacagacaaa
     9661 tgttaaagat atatatgctt gtggagatgt tgcacaagtg ggaaatatta gtttagcaat
     9721 atggccatct tcagtagaaa tgggaaaaat agcaggggca aatgcaagtg gagataattt
     9781 gacatttgaa agtgaagtat atccagtttc attagatgca atgaatgtaa aagtatttag
     9841 tattggtaat atacaaaatt ttgataaaga aatttcatca aaagatgaag gtcaaagaat
     9901 atataaaaaa ttatttatga aagatggttc attagttgga gctatactta taaatgattt
     9961 aagctgtact gttaaattga taagattaat atctgaaaaa ggtgattttg aggatattat
    10021 gaaggctgat atactataat ttaaatacat taaaataaaa aggagttgtc ttaaaattga
    10081 tttttttcta atcaaatttt aaattcaact ccttttttga atttttatat aaaataacat
    10141 ggaaatttta gtaatgtctg tggttacata cataggttta attgtttttt atatggattg
    10201 tctctggtat aataaattga attgtgtttg actgatagat atttttattg aaagttaata
    10261 cctgttatgc gataataagg acgtgttagg ggagttttta taataacaat taatctcagt
    10321 ttttgtgttc caatagtagc atcaacacat tctgatgtag tagttctttt cttataaaaa
    10381 ttagttacaa atatgcttgt aattatttga tatgtaaatt taccaatatt ataaatgatg
    10441 acctcgataa taaggagagt gaaaaataat ctatgaacga aaatattttg attgtggatg
    10501 atgaaaaaga aatagcagat ttaattgaag tatatttaaa aaatgaaggt tttacagtat
    10561 acaagtttta tacaggtaag gaagctctag aatgtattga atctaaaaat ttggatatgg
    10621 ctattttaga tattatgctt cctgatattg atggatttca tatttgccag aaaattcgag
    10681 aaaaatacta ttatcctatc attatgttaa cagctaaaat tgaagatata gacaagataa
    10741 tgggtttaac cataggtgct gatgattata taacaaagcc atttaaccct ttggaagttg
    10801 ttgcaagagt aaagacacaa ttaagaagat atatgcgata taataattct tatgaacaac
    10861 agtcgataat tgttaatgag tatgatatta aaggattgat tatcaataaa gaaacacata
    10921 aatgtagttt atttgggaaa gaagtagcat taacaccgat tgaattttct attctttggt
    10981 atttatgtga acatcaagga aaagtggttc cttctgaaga attgtttgaa gctgtatggg
    11041 gagaaaaata tcttgataat aataacacag ttatggctca tatagggcga ttacgtgaaa
    11101 aaattcatga accttctaaa aacccgaaat ttataaaaac tgtatgggga gtaggttata
    11161 caattgaaaa gtaaaaatat taaagtcaat cctgacttta ctcaagtgag aaataaaatt
    11221 tttatgagaa cagctttaat ggcatttata gccattattt tcatatggat tttatattct
    11281 tttatattgt atggtaagtt ttctatctgg atagtatcat tctttgaaaa tgcttttcgt
    11341 atggaatata aagctgcatt gaatttatat cagcagattt tccgtaatag tatggagctc
    11401 ttcatgatac tagctatggt gattgtgttt tttataatat tccgtgttta tttaaattgg
    11461 tttacaaaat attttagtga aataaatcaa ggtattgact ctcttatcaa agaagatgtt
    11521 ggagaagtgg ctttatctcc agagttattg gcaatagaaa agaaaataaa ctcaattaaa
    11581 catatacttg aacaacgtaa atttgagaca caaatggcag aacaacgtaa gaatgaactt
    11641 attgtttatc ttgcacatga tttaaaaaca ccacttacat cagttatagg gtatctgaca
    11701 ctacttcgag atgaaagtca gatttctgaa gaactgcgaa aaaaatattt atctatttcg
    11761 ttggataagg cagagcgtct tgaagattta attaatgaat tctttgaaat tacaaggttt
    11821 aatttatcaa atatatcact tgaatacagt acagtaagct taactcgtat gcttgaacaa
    11881 ctaacatatg agtttaaacc tactttgatg agtaagaatt taaaatatac actgaatgtt
    11941 acaccaaata tgatgataag gtgtgatgtt aataaaatgc aacgtgtatt tgataactta
    12001 ttacgaaatg ctgtttatta tagttttgaa gatactacta ttgaaataac agctgtacag
    12061 gaggatgatt atataaagct taaatttata aatcatagca atacgattcc agaggaaaaa
    12121 cttgagcgta tttttgaaca attttacagg ttggatacat ctcgtaattc aaacagtggt
    12181 agagctgggt tgggacttgc tatagctaaa gaaatagtgg agcttcataa cgggacaata
    12241 acagctcata gcgagaacaa tataattacc tttaaggtta ttattccgat tgtaggaaat
    12301 ttgtaagaat ttaagtataa aaaaatagga aattctttag gataaataaa taaagagcac
    12361 tcttgttatt gatataatta ctgtatcaaa acaagagtgc ttttattttg atacagtaat
    12421 tatatcaata gaaaaatgtg aaaatatatt atgtggaaca gatgcaattg taagtgaagg
    12481 ttgattggat ttgaaataga aaaagtattt taacatgttt ctgtagattg tataaaaata
    12541 aataaatcaa caaaggagaa tcaaagatga ataataaaaa aattgcagtt atatttggtg
    12601 gaaactcaac agaatatgat gtatcattac aatcagcttt tgcagtactt gagaatttga
    12661 atacggaaaa atatgatatt ataccaattg gaattacaag ggagggtaat tggtatcatt
    12721 atattggaga ttataataat atccagaata acacttggtt tgaaagggat actgatataa
    12781 ttccagttat tgtttcgcag aaccgtgtca aaaatggtat tattgaattt ttaattcctg
    12841 atgctgtttc tcagaatcat attccaaata gtgctattga atcatgtgaa aatgatatta
    12901 aatacactaa acttgatttt gtgtttcctg tactgcatgg aaaaaatgga gaagatggaa
    12961 cagtgcaggg gttatttgaa ttggcaggta tacaggttat tggatgtggt actctttctt
    13021 cagcattatg tatggacaaa gacaaatcac ataaacttgt tcattcagaa ggtattaaag
    13081 taccaaaatc aatattacta aaatactcag ataaatttaa tgtggctgaa ctgtcaaaag
    13141 acttaactta tccattattt ataaaacctg tgcgtgcagg ttcatcattt ggtattagta
    13201 aaatttataa aaaagaagag ttacaagtag cagtagactt ggcttttgaa catgataatg
    13261 aagtaataat tgaggaaaat attgatggtt ttgaggtagg gtgtgcagtt ttaggaaaca
    13321 atgacttact tgttggaaga gtagatgaaa tagaattgtc aaaaggattt tttgattata
    13381 cagaaaaata tacattgaaa agttcaaaaa ttcatatgcc agctagaatt gatttggatt
    13441 gtgagaaaag aatacaagag acagcaaaaa ttatttatag aactcttgga tgttctggat
    13501 ttgctagagt agatatgttt ttaactccat ctggtgaaat tatttttaat gaagtcaata
    13561 caattcctgg ctttacttct catagtcgtt atccaaatat gatgaaaggt attggactgt
    13621 cttttgaaga aatattggac aagataattg aggtgtacat aaaatgatga ataatatttt
    13681 attagataaa aataaagaag ataaaatgac aaatactatc ttattgacta aagataaagt
    13741 ttatgatggg aatctaattc tagtaaatgc ttttttacca gttaaaacat ctgaagatat
    13801 agatttaatt cctgtagata caaggtttcc aagtattctt atgaaacgtg aagctacaaa
    13861 cattttacaa aatattttaa aaagtatatg tggaataaat gaaatagtac ctgtaagtgg
    13921 atatcgtact gcagaggaac aacaagatat atattcaagt tctctgcgtg acaatggaaa
    13981 agactttact aaaaagtttg tagcacttcc aaatcacagt gaacatcaga cagggcttgc
    14041 tattgattta gggttaaaga aagaagttat agattttatc tgccctgaat ttccttataa
    14101 tggtatttgt aatgagtttc gtaaggcagc actacattat ggttttatag agcgttatca
    14161 gtgtaaaaaa gaagaaataa caggaatatc tcaagaacca tggcattttc gttatgtggg
    14221 atatccgcat tctgagatta tcaataaaat gaatctcgct cttgaagaat atatagaata
    14281 tattaagaga ttttcgtata caaattgttt aaaaatagaa gataaagaaa aaattataga
    14341 aatcttttat gtgcctattc atactaaaga agaagtgaga atctctatac ctgaacaatg
    14401 tgtatatcaa atatcgggta acaataagga tggttttatt gtgacatttt ggagaaatag
    14461 aaaggactaa gaaatggagg aacaagaatg attaggaata aatcttatgc agggcttgat
    14521 tattttagga ttatagctgc attacttata attgcaattc atacatctcc attattatca
    14581 tatagtaaaa tggctgactt tgtgttaact agtataattg ctcgtattgc tgtgccattc
    14641 ttttttatgc tatcaggctt ttttctaatt tcaacatata attataattg taataaactg
    14701 aaatcttttc taaaaaatgt attatttatt tacgggatag ctattgtaat ttatattccc
    14761 ttaaatattt acactggata tttttccatg aataatttag tgcccaatat tattaaggac
    14821 attgtatttg atgggacttt atatcattta tggtatcttc ctgcatcaat tattggtgca
    14881 agtattgcat actttcttgt aaaaaaacta ggtttaaagt actcactagt aatcactatt
    14941 gttatttatg taattggtat gtttggtgat agttattatg gttttttaga aaaaatacca
    15001 ctattaaaga atttatatgg gtatattttt gaagtatcag attatacacg aaatggtata
    15061 ttttttgcac ctgtattttt catacttggt ggaataatat ctaataaatc taattatatg
    15121 tcaactaaaa atagcttgat tggatttctt atatcattct tacttatgtt atgtgaggga
    15181 atgtttttac ataaactagg ggtacagcgt catgatagca tgtatattat gttattgcca
    15241 tgtatgtatt tcttatttac agccttaaca tttttgaaag gttctcgagc taggttattg
    15301 cgaacatcat cattaattat ttatattata catcctatga tgattgttgt tattcgtatg
    15361 ttttctaaaa tattcggaat acaaactgtc ttggttgaca atagccttat tcattatata
    15421 gtggtaagta tagcatcttt tatagcttct ataattatta tattgattct taaacctcta
    15481 aaaaaagact tgaaaggaga acataaggta aataccaata gagcttggat tgaaattgat
    15541 ttaaataatt tggaacataa tgtaaagatg atacaaaaag ctatgccaga agattgtgaa
    15601 ttgatggcag ttgtaaaagc taatgcctat ggtcatagtg cttttgaagt agcgacttgt
    15661 gcaaaccaaa taggtgtaaa agcatttgca gtagcaacaa tagatgaggg gattgattta
    15721 cgtcgatatg gaatactagg agaaatattg attcttggat atactaaccc aataagggca
    15781 aaggaacttc ataaatacaa tcttacacag agtgttattg accttaatca tgctatctct
    15841 ttaaacaaac agaaatacaa cataaaatca cacattaaaa ttgatacagg tatgcacaga
    15901 ctgggttttg atgttaatga tgtgaggtca attttaaagg catttaaatt aaaatactta
    15961 gatatatgtg gcatatttac acacttgtgt gtttcagata gtctgaaaga tgaagatgta
    16021 gactttacaa ataaacaaat tgaatgtttt tataagttaa ttgattttct gttaaaaaaa
    16081 ggtataaaaa ttcctaaagt acatattcaa agtagttatg gtttttttaa ttatcccaat
    16141 cttaagtgta attatgtaag aattggaatt gcattatacg ggatattaag ttttccaaat
    16201 gatacgacta aattgcaact tgatttacga cctgttttat ccttaaaatc acaagtaatt
    16261 cttattagaa aaatacaaaa agatgatagt tttggatatg gaagagtctt tattgcaagc
    16321 agagatagcg taattgctat attatctatt ggctatgccg atggtatacc tagaaattta
    16381 tcctgtggaa aaagttacgt aataataaat ggttgtagag cagcaatagt gggtcgaatt
    16441 tgcatggacc aacttgctgt tgatattaca gatataccta atgtagaagt tggtaatacg
    16501 gcaataataa taggaagaga taatttatct gaattatctg tttctgaagt tgcaaataac
    16561 tcatgtagta tatcgaatga attattgagc agagtaggaa gaaggttaaa tgttataaag
    16621 aagtaactca attaaatagt aatatttgtt gtactgtgta atttatttta ctctgactag
    16681 acatttttag taatattatg tctaatatat attttttata acttacatga caatttaagt
    16741 tataaaaagg tgatgtttca gatttttatt ttgaaacatc accttttttg ttataatata
    16801 taataaaata attatacctt aactgtaaac ttatatagat tatttacttt ttaatattag
    16861 attcattttt tgagattggt atataataaa catatagagt atataataat agttaaagtt
    16921 aaaaaagggg aaaagcaata tgcagtatat acaagaacta cttgatttga ctcaaaatta
    16981 ttgggtgtta gccacaataa tcggcttatt aagtgcattt atagaaagct ttataccagc
    17041 cttaccactt gttgctatag ttacagcaaa tgctgcaata caaggattat ttattggatg
    17101 tttgttatca tggattggct caggacttgg gacaacttca ttattcttat taataagcag
    17161 atttaatgat agtaagcttt ttaataagtt gagaaatagt aaaactgaaa aagcaataag
    17221 ttggatggat aaacagggat ttaagttatt atttatagcg tatgcctgtc catttatgcc
    17281 cggttgttta gttactatcg catcagcttt tggtaaaaaa gatttaaaag attttgtgcc
    17341 agcaatgttg gcaggaaaat ttgtaatgtt tatagttata agttacgtgg caagtgatat
    17401 agaaggtttt ataacaagtc ctttaaagat agcatcattt atattattgg tgtttttgtc
    17461 atggaagatt ggaaatagag taaataaaaa tcttgaaaat cataattatg atttccatca
    17521 taaaaaacat cacaatgata aagatgataa aatgatttag cttgtacgaa ataaaacgta
    17581 tcttaaaatg atttagtact aaattatttt aagatacgtt ttattttttg tattaaggta
    17641 caaaattttt agttcttttg taaactaaaa tatataaaac ttatttatac ttagttcaaa
    17701 atattgttat ttattttttc tacaactata ataaaatata aaattgatat ccaatcaaaa
    17761 aaataactat gaaatttaat agcttagata tgaatttttc tgtttttgag ttagttttta
    17821 taggtagtaa agaaatgttt atagtaatgg gataaaacaa ttcaatacct tctttagtaa
    17881 taaggtcaag acacaagtga gatagatatc ctattataaa cccactggac aaacatgaga
    17941 aaactatatt attgttagtg aagattgtca gagatttaaa aaaactataa ataagtaata
    18001 aggcaattag actgtgagta aaccctctat gtctaaacct acttccaaat aatttgtata
    18061 ttaacataaa ttttttactt atgtaagaac ctctcatatc tatatctgga aataaagaac
    18121 ctaaatatgc aaaataaata taaattataa ttaatactat tttatatgca atatcgtatt
    18181 taactaggta cagattgttt ataaagggga gtatcaaaag tgcgaatata taacctcccc
    18241 tttcatgtgt ttcttttatc atataaaaat cctcctgaat cgtcactata aacattatat
    18301 tggtaaattt agactatctt ttattatatt atatagcaaa cataagtttt tttcaaaagg
    18361 attttaatat ttaattaatc ttgagatttt aaatttttgt aattttctaa agcaccattc
    18421 caatttacta cattaaacca gttgtcaatg tattcatttc ttctattttg atattttaaa
    18481 tagtaagcat gctcccaaac atcaagtcct attataggag ttaggttttt acttacaggg
    18541 ctatcctgat ttggagtagt cataatagat aatttcccat ctttagtagc tacaagccaa
    18601 gcccaaccag aaccaaagac atctaaagca gatttttgga actcttgctt aaatttttca
    18661 aaagaaccaa agtctctatc aatagcttct tttaaagatt cagaaggtat ggttttttct
    18721 ggcgtcatta tatcaaaaaa gaatttatga ttataagctc cacctgcatt atttcttaca
    18781 gttgtagcaa tatctttagg taaagaatct aaattttgca ataattcaca taaagaataa
    18841 ttataaagct caggatattt ttcaagagca gcatttaatt tatcaacata agcttgataa
    18901 tgcttatcat gatgcagttt cattgtttct ttatctatat aaggttcaag tgcatcatat
    18961 gcataaggta atggtttaac cttaaatttg ttattttcag gtgtaaaagc aaatgaagtt
    19021 atgcactgtg agattataaa taaagacata ataactggta ttaatatttt tttcttcata
    19081 aaaatcacct cctaatatag tatttctaga aataaaaaaa atatgaaata ttaacagata
    19141 atttataaga atttaatagc aatttcattt taaattgaat aagttttaaa gccaaagaca
    19201 aaactatgaa atatagaata agactaaagg acagaaagga attttgatta tatggacggc
    19261 aatatttcaa tttattataa aggtgatata tctaaaaaag aagatattaa atttaaaata
    19321 aaagaatctg taaataatac aagactttct agtagaatag agtataagga tgaggaaaaa
    19381 acttttgatt ttgaaagatt taatgtaatt gatgctattt atggaaataa accatttgaa
    19441 aaagatggaa aagttattgt ttttaatgga aatatatata actatcatga aataaaagaa
    19501 gaattaatta aaaaaggaca caattttact acaaatgatg atattgaagt cttattggca
    19561 tcttatatag aatttggtaa gaattgtgta cataagatta aaggaatgtt caactttata
    19621 atctatgata gagaaaatga aagtatattt ggagcaagag atttatttgg gataaaacca
    19681 ctctattata taaataagga aaatgcaata attttttcct cagagtataa gtttttactt
    19741 gaatatatga aaaatttaaa tataaatgaa agaagcttac aaagttattt ttcatttcaa
    19801 tatgtacttc ctgaagatac tatgatacaa ggaataaggc tgataccagc aggtcactat
    19861 tttagggtag aaaatggtat actatcttta aagagatata ataaacttga gtttagaagt
    19921 agtacaaaat ttttttatac taaaaatcac ttaggcaata gagatgtgaa cgaggatgat
    19981 ataagaaata tagtagtaga ttcaatagta actcatatgg aagaggagaa agaaattgga
    20041 acatttctgt ctggtggaat agattcatct attataacta ctgtggcttc acaaattaat
    20101 ccaaatataa aatctttttc tgcaggcttt agtgtcaaag gatatagtga attagaagta
    20161 gcaaagaaaa cagctgataa gttaggtata gaaaatatcc agattaatat aacacaagat
    20221 gaatatataa aatcattacc gaatgtaatt tattctttgg atgaccctat agcagaccct
    20281 tcagaagtag gtctttattt cttaattaaa gaggcaggta aacatgttaa agtagcattg
    20341 tctggagaag gagcagatga attatttgga ggatataata tatataaaga gtatagtact
    20401 atgaaatcag ttgtcaattc acctacttat attaaaagta tattaggaag agtttcagaa
    20461 cttatgccaa acataaaagg aagaaattat ctttatagag ctacaacacc attagaaaaa
    20521 agatatatag gaaatgcaaa agtttttgaa aacagcgaag taaaaagatt cttttttaag
    20581 tataaggaaa agaatatata tgaatacctg ttgtctaatt tgtatagaga tgcacaaaaa
    20641 aataattacg attatataag taaaatgcaa catatagatg tgaatacatg gcttcaaggc
    20701 gatatacttc aaaaaattag caaactttct acagctgagc aggttgaact tagagtacca
    20761 tttttatata aagatgtatt tgatgtagca aaaaatttaa gaatggagca aaaaataaat
    20821 aagaataata caaaagtttt acttagagag gcgtttagag aaatagtccc agaacatgtt
    20881 gttcaaagga aaaaattagg atttccaact ccaataagag tttggttaaa agattctcta
    20941 ggggatattg taaaggaaac tatttataat tcaaatgtag atgaatttat agataaaaaa
    21001 tatgcaataa aacttttaga tacgcatctt aaaggtcata gagataattc gagaaaaata
    21061 tggactatat ttacattttg tctatggcat caattattta ttgagcataa aaatgtcgag
    21121 tattaatact tttttcaata ttaatcagag ggcaaaagca tgcaatgttt tcaccctcct
    21181 gattattatg gaataggata agtagaattt tgattgatta gtttactatc tcaataaaat
    21241 tattggcaaa tattttgaca agattacttt caacagctaa atttaagttt tgtccatcat
    21301 catatccagc ggaaatcaat cctagtaagt ttccaaacat atctaaaaga gctcctcctg
    21361 aactaccact agagattgga gctgtaaatt gtatcatttg aaccgtttca aaatcacgaa
    21421 aagctgaaat aattccatct gaaactgtat taaaaagacc tagagggctt ccaattgcaa
    21481 ctattttttg acctctcaca ggttttttac tgactttaac aggtattggc ttgcattttc
    21541 tatcaacctt tatcacagct aaatcataat ctgaatggta ttttataatt tggtaagatg
    21601 tataaacttt atcatcattt tcaaatctaa ctaaaaatga atacccatca ttgactacat
    21661 gaagattcgt aagtatataa ccttcattat ttattactac tccagaaccg ctcttaaacg
    21721 gtttattttc gttattgcac acaacaatca taacaacaga agctgataac tttgcaaggt
    21781 cttcataaga aagttctact tcctcatctt gtacaatttt tttagatgtt agatttgtgt
    21841 tattaagtgt atttttagag ttaggtttat tcgtagtagg agaagtctta ttggtatttt
    21901 cactgtaatt tatatttgta atatgccttt caaaaggtat ttctaatata tttggagtct
    21961 tcctagaaag ttcaaataga gtagaaaatt ttgaacattc aggatataaa tcaagaatat
    22021 ctttatttgg atttatatat aaaatatcac acccaagttg tgataaaaaa tataagaaaa
    22081 aaagttcttg ccttttaata tttccaaagt atactatctt tggtgaaata gtagtcttca
    22141 ccttgttata aaataattta ggtagaaaat agtctatcca acacaaaagt ttaactgcaa
    22201 agtttttgat gatagaggat gtacaatttg gcatacttgt ggaaaataaa tctaaagtat
    22261 ttttaaatgc aatcttttta gtccaattta aggtgtcatt ttctatttta catggaaagt
    22321 cgatattata taaatcccaa ctgttaaact tagaactatt ataaatatta aaaattttaa
    22381 gcatcttatc tgtttgttct ttgtcaaaat ttatttttag attgtttaat ctaatatagg
    22441 gaattgtagg attattaaaa aaataagtat ctatattttt aattttttct atgtatttat
    22501 caattgaatc atcaacacct atatatcttg taaagtagac ttctttaata tatgaagaac
    22561 gcttattttt ataggtagta tcagatataa attcaattag aggttcagta gactttttta
    22621 ttgaaatctt taatggttta atagtacaaa tcatattttt tctcctataa taagatgata
    22681 taatctatta taaaacaact taaaacaata tagtacaata attttgtagt ttcaaaataa
    22741 aatatgtact tgtttatata aatgatatat aattacatat taaacatagc ttttatgcat
    22801 ttaataaagt atgtaaaatc tatgtaaaaa gtaagaaata taaacatttt tttaattata
    22861 ttttaaaaat atcaagcaag ttttattcac aaatgaataa aaaatgttat aatatgatta
    22921 aagaaaaaga taaataatta gcaagatttg tttagctaaa attaacaagg gagcgataaa
    22981 ttatgggtat aacattatca aaaggccaaa aggttgattt aactaaagga aatccaggtc
    23041 ttaaaaatat tttagtaggt ttaggatggg atactaacaa gtatgatggt ggatttgact
    23101 ttgatttaga tacagcagca tttttaacag gagcaagtgg aagtgttact aatgatggag
    23161 attttgtatt ttacaacaat ttaaaacata catctggagc agttgagcat ctaggagata
    23221 acagaactgg cgaaggtgat ggagatgatg aacaaatagt tgttgattta tctaagattc
    23281 ctggagaaat aagcaagatt tcttttactg ttacaattca tgatgcaaca gaaagaagac
    23341 aaaattttgg acaagtaagt aattcttata taagaatagt agataaagac acaaatgaag
    23401 agttaataaa gtatgaatta ggagaagatt ttagtataga aacagctata gtagtagctg
    23461 aaatatataa gcacaatgga gaatggaagt ttaatgcact aggttctggc tttgaaggtg
    23521 gtcttgctgc attatgtgga aactttggaa taaacttata atttcaacta taacaataaa
    23581 ctaaaggggg atattttcca tggctatagt attaaaaaaa ggacaaaaaa tcgatttaac
    23641 taagggaaat ccaggtctta aaaatataaa acttggacta ggatgggata caaattcatt
    23701 tgatagtggc tatgattatg atttagatgt aagtgttttc atggttggag aatctcaaag
    23761 agttgaaaaa gatgaagatt ttatattcta caataactta aaacatccat ctggagcagt
    23821 tgaacactta ggtgataata gaactggtga aggtgatgga gatgatgagg aaatattagt
    23881 gaatttagag acaatgccaa agcatataca aaagatagct gtaacagtta caatatatga
    23941 ggctgctgaa agaagacaaa attttggaca agtaagcaac tcatatataa gagtattaaa
    24001 ttctgaaaat gatgaagaaa tattaagata tgacttaggt gaagaattta gtatagaaac
    24061 tgcaattaca gtatgtgaga tatataagta taatggagaa tggaagttta gtgcagttgg
    24121 agcaggattt gaaggtggct tagaagcact ttgtagaaac tatggcttaa atgtttaatt
    24181 taagcaatat aatatattaa atataaataa cctatacaaa taatgtatag gctatattat
    24241 ataaaatttt gtggaggttt agtatgtcga ttaatttatc aaaaggtgat aaaatagatt
    24301 taaaaaaatc taacccagga ctgagtaaca tacttgtagg tctaggatgg gaccctgtgc
    24361 aacaatctgg cggaggtttt tttaaatcgt tatttggtgg aggacaagct gatatagatt
    24421 gtgatgcatc tgtatttatg cttaatcaag aaggtaaatt aagtggaata aaagatttaa
    24481 tttattttgg aaatctaaaa agtgcctgta aatcagtttt gcatacagga gacaatctaa
    24541 ctggtgaggg tacaggtgat gatgaacaaa tcctagttaa tttggataaa gttccatcaa
    24601 atattcataa attattgttt gtagtaaata tatacaattg tgtagataga aaacaacact
    24661 ttggaatgat agaaaatgcc tatataagag ttgaagacca aggtaataag aaagagatag
    24721 caaaatacaa tctatctgat aattattctg aaaaaactac tcttatagta ggagctatat
    24781 atagaaaaga tggaagttgg caatttaaag caattggtga aggaacaaaa gatgcgggtc
    24841 ttaaagaagt aatgcaaaat ttagacagga ttgagtgtgc atatggaatt taataatgat
    24901 atcaaatatt ataatgtaga taaagaagaa gttacaaaag acttatctgt taatcctgaa
    24961 aaaggtttat cagaatcaga agtcaagact agaagagaaa aatatggttt gaatgaattt
    25021 actccaaaag aagagggtag tttttgggat gatttaaagg aaagtttaag tgaaccaatg
    25081 atagttatct tgattattgc agcagtagta agtgcagtaa ttggggaaac tcatgatgca
    25141 atagggattg taggtgctat agcaataggt atagcaatag gtatgattac agaaggtcgt
    25201 tctaagaaag cagcagaggc tctttctaag ttgactgaaa acatagaagt aaaagtattg
    25261 agagatgggg aagtacatca aatttctaag agtgaattgg taccaggtga tatagtatat
    25321 attgaaactg gtgacatggt acctgctgat ggtagactta tagaaagtat aaaccttaaa
    25381 attagagaag atatgttaac aggagaatct gatgacgtat ctaaaaagtg tgatgtagta
    25441 gtttctatgg aagagataga atctaaagga gcagtagtag tacaggaacc aatacctgca
    25501 aaacagataa atatggtgtt tggtggtaca cttgtagctt atggtagagg agctctagtt
    25561 gtaacttcaa ttggagataa tagtgaaatg ggtaaaattg ctcaaaactt atcagaaaca
    25621 gatgagcaaa ctccacttca gataaaactt ggaaatttag gtggtttgat agccaaagtt
    25681 tctagtgcag tagcaggatt actatttata tttatggttt tccaaatgat aagtaagaat
    25741 gtattaaatg tagatatgtc tggaatatta ccgtttttag aatcaataga cccagttaaa
    25801 acagcattta cagtatgtgt tgcgttgata gttgcagcag tgccagaagg tcttccaact
    25861 atgataaata tgacacttgc aataacaatg caaaaaatgg ctaaaataaa tgcattggtt
    25921 accaaaaaag aagcttgtga aacgataggt tctgtttctg taatatgttc agataaaact
    25981 ggtacattaa ctgaaaatag aatgactgta gaagttgcat atgttgatgg cagatatatt
    26041 gaaagttcag aagaggaaat aaatagttat tttgaagaaa actgtatgat aaactctaca
    26101 gctgatgtag agcacaatga tggtgatatt aagtatttag gaagtgctac tgaatgtgca
    26161 ttgcttttat attacaaaaa cgtagattat agacaagtta gaaaaaattc taatatagta
    26221 gctcaaaatc cttttacttc agattcaaag agaatgactt ctgttatagg tcaagataat
    26281 catcatgtac tattatcaaa aggagcacca gaagtcttac ttgaattatg ttcacatatt
    26341 caaagaggaa aagacatagt tccattaact gaaaatataa aacatgagat tttagaagaa
    26401 ataaagaaat tgcaaataaa atcaatgaga actttagggt ttgcatataa agaaatctca
    26461 caagctgaag aagaagcagc agtaactgct gaaagtgatg ctatgaatgt aagtgcaatg
    26521 gaaaacaatt tagtatttag tggttttgtt ggtataagag acccacttag aaaagatgtt
    26581 atagagtctg taaatacagc aaataaagct ggtgtttctg taaaaatgct tacaggagat
    26641 aatataaata cggcaagagc tataggtgaa gagttaggcc tattaaaaaa taatatgaga
    26701 gtggttgaag cttcatatat agatacttta gatgatgaag aattaaaaca agagatacaa
    26761 agtatttcaa tcgtggcaag aagtaaacca gatagtaaaa tgagaatagt ttcagctctg
    26821 caaaagagcg gagatgttgt tgcagtaaca ggtgatggta taaatgatgc tccagcactt
    26881 tcaaaagcag atgttggtat agcaatgggt atatctggta cagaggtatc taaaaacgca
    26941 gcagacataa ttttaactga tgatagtttt agtaccattg taaaaggtat taaatggggt
    27001 agaggaattt atgataactt ccaaagattc gttcaattcc aattaactgt aaatgtaatt
    27061 gcatttttag tggctataat atctcaagtt atgggacaag agatgccatt tactacaata
    27121 caattattat gggtaaacat cattatggat ggaccaccag cactagcatt aggtttagag
    27181 ccagttaggg accatgtgtt aaacagaaaa cctgttgata gaaaagccaa tataatttct
    27241 aagtcaatgg tgtatacaat agtattaaat gcattttata taacagcaat actaatgtta
    27301 caatctacat ttaatttctt aggagttgat gaagcgcata agggaacagt aatgtttgcg
    27361 ttatttgctt ttagtgcact gtttaatgca tttaactgta gagagtttaa tgcagatagt
    27421 atcttcccta attttacaca taacaaatta gcactacaaa taatagtgtt gacaggaatt
    27481 gcacaagtaa tatttactca gctattccaa gatttcttta actctgtagc aatggatttt
    27541 atgacatggg taaaagtttt atgtgtagca gcaagtgtaa ttgtggttaa tgaagttgta
    27601 aaatgtataa taagactggt aaaaccaaaa aagactgctt aaaacaaatt ttttagaaga
    27661 aaacagactg actcaaaata tgaattttgg gtcagttctg aataaacatt taaattggga
    27721 ggatagttat gggtgactca aaggtatttg aaaaaatatt ctccagccaa tcagatagag
    27781 gaaattacac tccttcaaag ggatatctaa gttattttat ttcgtatatt ggtttagaag
    27841 atgaagtttt gtacaatctc gagatattta aaacaaaaca aaatattgat agcaaaaaag
    27901 atattgcttt atttactgat gttatagcaa atcctagtga ttttgatatt ataaattatt
    27961 ttaaaagtgg acttcaaaaa tatagaacta gcatggaaga tgtagatata aatattttgg
    28021 gatttgaaga gattgattat aaaataaagc aagcaatgga tagagtgtta aaagaagagg
    28081 aaaaagaatt tactaatgat agagttaaac aaaattttat agtgaaaata atggcatgga
    28141 taaaaattta tatcggtgct ttagatataa ataaaaatga agctccaaaa gtaatatttt
    28201 atggagatat aaaaaaacat gaagtttatt tactcttaat attatacttg gcaggttttg
    28261 atgttttata tcttaatcca aattcaaaga gtaatataga tattttaaaa tcagaaagat
    28321 acaatataga gtttgaagaa gccaacataa tagaagaaaa aatttctttt gaagaaaggg
    28381 ttattttagg ggaaaaaata gataaaagtt ctgtaaaaaa agcttttact gttggggcag
    28441 aagcatcaaa gcgtataagt gaggaattgt taaatgatgc aggatttata aaaccttggc
    28501 aactccaaga tagaaaaata aaaaatctac ttctaagttc aactgttgat gaaatatcaa
    28561 tatactggaa tcaaccactt aaattaagac caggatttaa gtttaatgat gctattgtag
    28621 aagctccaaa ttttctttct aagattaatg gaatatataa cgataagaat gaatatatta
    28681 agtttttaga tttattaaga gattcggaaa gttcaacatt tattgaattt aatggagatg
    28741 tagatagatt ttcaaaagct tttacaagag aagcttttag tttatccttt ttattggatt
    28801 caaaaggtgc aatagataaa aactctgttt taaataataa agattattcc atatcaacat
    28861 tagcattgaa tcaacaaatt atgattttag agaaagtgga agagttgtta gaaggaagta
    28921 tgtttttgaa tggtttaagt ggagaagaca agataaaagg tctatttaca gtacttcaca
    28981 tggataagaa atttgttcat atgatgaaca atttcgatta ctcacttata aacccaaaat
    29041 tgattatata catgtacaag agtattgtat ttgataaaga aatagttttt cttatgcttt
    29101 tacttagtaa gattggattt gatattatca tattatgtcc aggaggagaa aacaatatag
    29161 agaatgttat aaataaccaa ttgattgatg tacacagatt ggataaaatg gtatatgacc
    29221 ttaaattaaa ttcgcttgaa aatgatatac ctttacttaa aaaaattttt ggaaagagga
    29281 gacgatttta gatggggata aatataaata gtttttcaaa agataagaac tctgtagagg
    29341 ttactgagaa tttgcctaca gcagttcaag aagaaaactt cgatataatg gaatatacaa
    29401 ataataaaaa aaatgaactg agaaagtcta aggaagttga ggcattgaca tctcttatag
    29461 aagtagaaaa tccagatacg attttacagt ttggtaggaa agcatctgaa ggagtagcaa
    29521 gagtttctga tagcctttta aatacaataa aattaaatag aaatgaagaa aattctaaaa
    29581 tgctggttca tttaactaaa ataatggaca agtttgattt agacgatttt caagaaacta
    29641 aggaaccaaa tttcgttcaa aaacttttca aaaaagctaa taatgccatt gaaatgatgt
    29701 ttcaaaagta tgaaacattg ggaggagaag ttgagaaaat tcaaattgag cttgaacaat
    29761 acgaaagaga tatagcactt tctaataagc aaataggtgc tatgttaaat gaaaactttg
    29821 agttttacaa tgaacttcaa aaatatattg ttgctggaga aatggcaata gaagaaatgg
    29881 ataatgaaat actacctttc tttaaacaaa aatcagagac tagtggagac caaatggatg
    29941 ttgtcaatta tcaagaacta ttaaaagttt atgatatgtt aaatcaaaga gtctatgatt
    30001 taaggatagc agaaaatata gctatccaaa caatacctat gcttagaggt atgcaacata
    30061 ataattatgg tttaattaga aaaataaata gtgcatttgt cgtaacttta ccagtattta
    30121 aacaatgttt atctcaagct atattactta aaaagcaaga attacaagca aaatctctaa
    30181 aagcacttga tgacaaaact aatgaattat tgcttagaaa tgcacaaaat gtaagtactc
    30241 aaagtgcaca aatagctaga atggctggaa caagttctgt tcaaatagaa acattggaaa
    30301 aaatgtacaa tactattaaa tctggtatag atgaaactat gagaatagaa gaaaacaata
    30361 gagctttaat taaggataat acaaagagat tggaagaatt aaatacgact ataatttata
    30421 ataagtagta ttatattaag ggctaatttg tataaagttg gccctttcta tgtatatgag
    30481 gaggctagac attgaaatat ttcgattata tctgcaaaga tgatttggag agaatttttt
    30541 tgaaggagcc agaagatttt agtgctaaga ctgaaaaaga tgtgttgaaa tatgcactgg
    30601 gcgctttttt atacgttcca gctactcaat ataatatgat ttataagagt attatagggg
    30661 atgttaaggg agttagacca ttagctattt gtttagaaga tgctgttgga gtaaatggag
    30721 agttagaggc tatagaaaat cttagattaa ttttaaaaaa tattagcaat gaaagtataa
    30781 ctaataaaga tggaatacca ctaatttttg taagaataaa agatgtggag caactgttga
    30841 gaattaaaga gattataatt aaaaatagac attctataac aggaatatta attcctaaag
    30901 caaattcgga gttaatagaa aattgtatag aagctttaga ttctatgaat cttcaagata
    30961 tgtatgttat tccgataata gagacaaaag aatttatata taatgaaaaa aaagaactta
    31021 gttttactaa tttgtacaat gctattttaa gacataagtc aagaatatta agcattagag
    31081 taggcataac tgatatatta gggatgtatg gtataaggag agataagaac ttctctatat
    31141 ataataattt gatttgttct tcatttatat tagatataat cacatatctt caaagaccag
    31201 agttagatat acctataagt ggtggagtat cagaattttt tgatatgacg aataaggata
    31261 taagaaataa gtatatagaa gaaatattat tggataaata tcatggatta attggaaaga
    31321 ccgtaattca tccaatgcaa attcaaattg tacaagcgct atctacagta tcatatgaag
    31381 attttactga tgctttggat atactggata gtacagacag taagtatggt gtgagcaagg
    31441 gtgtattagg agagagaatg aatgaaacaa atccacattt tttatgggct aaaaaaacat
    31501 tgattctatc taaaatatat ggtgtattaa ataaaggagt agactatgaa gaattgctta
    31561 aattttaatt gtgagattga cattattgat aatccattga atttagaact aaatgatttt
    31621 ttagacgtag gaataagaaa taatagcaaa agacgttttt tgtttatctc aaagaaactc
    31681 ggtaaacatt tagcatgtaa gccaagtgag atggataatt tgggaaaatt aatggctaca
    31741 atttataatg aaaaaaataa ggaagaatca aatggtactg ttatagcttt tgctgaaact
    31801 ggtacagctg ttgggcatag tttttttgat tacttatgtg gagattatga atttattcat
    31861 actactagag agcagtttga cgagttagaa aatctggaat ttttagagga acattcccat
    31921 gcaacagacc agaatttata ctttggtatg cttaacaact ttaaatttgg agatgagata
    31981 atattggttg atgatgagat taccacagcc aatacatgca tgaatataat taggaaaatt
    32041 caaagcattt atcctaaaaa gaaatacact atatgctcaa tactcaattg ggtaagtgat
    32101 gaaaatttaa gtaaagttac tcgtttagaa agagagctag attgtaagat agattttgta
    32161 tacttattta aaggaaactt taaattttat atagatgaag atattatttc aaattacaac
    32221 gataattata gtattactaa attagagact atttcaagtg ataaagtaaa tttagtagta
    32281 aatcatattt atgttgattt agaagattat attggaaata agaaatatgt aaaatataca
    32341 ggaagatttg gaattaatag aagagaacaa tttagattaa aagaaatcgt taagagagaa
    32401 agtaaaaaaa taactatggc cagtaaagaa tctttaaaaa atgaaaaaga aaagattcta
    32461 tttttaggta ctgaagaatt tatgtatata cctatgctgt ttgcaaaaga aaaagaaaat
    32521 caatatgata tatactatca ttcaacaaca agaagtccaa ttattaatat tgataagaat
    32581 aattatccta ttaaaagtaa atttatgcta aagagttttt ataatataga tattcaaaat
    32641 tatatttaca atatagataa acataatttt tctaagtgtt atttatttgt agagctgaat
    32701 aaaagtgaaa attcttatct agaatttatt gatataatta aaaaaacaag tataaaagaa
    32761 ttaactatag tatgttgtag ctaaagtgtt ttaacaggat aaaatatatc acgattttaa
    32821 gataggagtt gagtcatatg agagatttta gcacgtattc taaagatgat gttatttttt
    32881 tattaaaaga tatatcaaat ttaattgaag aagaaggaaa taaagaaaga gaattaaaaa
    32941 tacaaaatgg tagacattat tcagaaatga taccaataga gtatgaagtt agtgaagatt
    33001 atctgaattt atatcatgag aaactaagag aaaataaaga taaacttgca ttttctatag
    33061 gagttatgtg tgaaaaaata attaaaaaaa atggaaatga tgtagtacta gtttcacttg
    33121 ctagagcagg aacacctatt ggtattttgg caaaaagata tataagaagc aagtataatg
    33181 taagtttacc tcactataca atctctataa taagagataa gggaatagat atgaatgcaa
    33241 ttgaatatat agtaaagaat catccgagct caaaaattca atttttagat ggatggactg
    33301 gtaaaggaac tatatcaaaa gaacttgaaa aagcatgtga tgaacttaat gttaaattta
    33361 aaaaatattt tgactcaaca cttgctgtat tagctgaccc tgctggatat tcaggacttt
    33421 atggaattag agaagatttc ttaataccaa gtgcatgttt aaattcaaca gtttctggtt
    33481 tagtaagtag aactgtacta agagaggatt taattagtaa aaatgacttt catggagcta
    33541 agttttatag acatttaaaa gataaagatg aatctatgaa ctatatcgaa actatagaag
    33601 aatgttttaa aaatcaattt aagaatatat ctgatgaagt tgaaaactgg gaaagtgaca
    33661 ttataacaag agatggatac tttgatgttt taaatataaa agaaaagtat aacataacag
    33721 atattaactt tatcaaacca ggtgtagggg aaacgactag ggttttactt agaagagttc
    33781 cgtataagat tcttgttaaa gacttaaatg ataaatcttt agaccatata ttcatattag
    33841 ctaaggagaa aaatgttgaa gttgaacaaa tggatttaaa agcctataaa tgttgtggaa
    33901 taataaaaaa tatgaaagat atttaggaaa tatattagac aggagaatat tgaatgattg
    33961 ttttttcaga tttagacagg agtatcattt actccaataa atttttaaat gctgatagta
    34021 aatatgctaa tatagaaatt tatagggaaa aagaaatatc ttatatatct ttagatacta
    34081 taaatttaat aaagcaaata caatattatg gtatgtttat acctacaact actagaacag
    34141 tggagcaatt taagcgtata gaatttaata aatatgggat ttattttcct tggagtataa
    34201 cttcaaatgg aggagttata cttaaagata atgaaatatt gaaatcatgg agtgaaaaaa
    34261 tagataaact aaaatctgat tatgagccca ttgaaagtat gattcataaa tttaaagact
    34321 atttgaatgt agatggaata actaatttta aagttgcaga agatacattt ttttatatag
    34381 ttgttgattt atcaagattt aatttggatt caatcaaaga atatacaaat atattggaat
    34441 caaaaaattg gaagttttat gtgagtggca gaaaaatata ttttatacca aaagaaattt
    34501 caaaagaaaa tgctataaaa tacttgacta aagaattagg aatagaatat ttttatgcag
    34561 ttggagattc tattatggac tatggtatgc ttaatatctc aaataaatcc tatgtattga
    34621 agcatggtga tataaataaa aatgaaatag aaaattcatt tatatcaagt tctttcaatg
    34681 gaatgagtgg aactgaagag atactatcta acattttaga cgagaactgc ctacttaata
    34741 tataatgact gatatataaa taataaacta gaaaaatctg aaataaatta tagtaaagtt
    34801 tatcatacta atttatttca gatttttatc aatttatatt taagtaagaa ctcaatatgt
    34861 taaaatgaag gtggagtcat tgcccagact gagatacata cctcatcatc ataattttca
    34921 tacacatgtg gtatagtact gttaaaataa aaactatctc cttcctccaa tagatattcc
    34981 ttatttccaa gtgttatctt cattttacct tttattatat agccacattc ttctccttcg
    35041 tgtgtcacaa attcactgtt tttgtgtgta tgaccatcta gtgtaacaag tataaattct
    35101 atttttttcc cagataaatt aggaacaaga agttcgtaaa attttgtaga atcattagtt
    35161 actatttttt ttcgattatt tttctttact actatatcag agtcttctct tccaatctct
    35221 tcaaaaaaat aacctatatt tatatcaaga gcttttgata ttttccacat tgctacaata
    35281 gatggaacaa ccatattacg ctcaatttga cttattaagc ctgtacttaa atctgaattt
    35341 tttgctaaag tagagatact tatattttta gattttctaa gagatctaat tttgcttcca
    35401 acatctatat tcattagata caaaccccct cagacatgat tataacatat aatttataaa
    35461 gattaataaa ataaaaaaaa agttttacaa tacaagtatt ttttatgcta tattgaattt
    35521 tctgaaaaga tgcattataa tagtattaat attcaatata ttgaatattg ataacgatat
    35581 tttaatttaa tgaaatggag gtaattaaga tgaagagttt tgtaagtcct gataatattt
    35641 gggtattatg ggcagttgta actggatggg cagcatttag tatttatttg gaacaaaagt
    35701 acaattgggc atcaaaagtg tcgggagcaa taatagctct tgtaggagca atgttattat
    35761 caaatctaaa tattatacca gtagagtctg ttgtatatga ccaagtatgg ggatatgttg
    35821 ttcctttagc catagcttta ttgttatatc aatgtaacat taaaaagata tggaaagaaa
    35881 gtggaagatt gctcattata tttcttgtag gttctgtagg aactgtactt ggagctatga
    35941 ttggattttt agcattaaaa aatgtagtac cagatttaaa tattgttgca gctatgatga
    36001 caggttctta tataggtgga aatgtaaact ttgctgctat gtcaggggcg tttgatgcgc
    36061 caggagaatt agtatcagca acagttgtag cagataatct tttaatggca ttatatttct
    36121 ttgtcttaat agctatacca tcaattggat tttttagaaa acattttaaa catccacatg
    36181 tagatgaaat ggaaagtata ggtataactg aaggagaaac agtagcagca aattattggg
    36241 gaagaaaaga aatatcttta aaagatatag caatagccat tggtagcgct tttataattg
    36301 tcgcagtatc tgtagaacta tcaacttggt ttaaagctat aataccatta tcaaatcctt
    36361 ttttagcaat gttaaatact ttatttggaa atcaatatct aataataact actttgacta
    36421 tgttgtttgc cacatttatg cctaatttct ttggtgatat aaaaggagca caggaattgg
    36481 gtacattttt aatatatata ttctttgttg tagtaggtgt tcctgcttca ataacattaa
    36541 taattcaaaa atcaccatta ctacttttat actgcggaat aatggtacta ataaacatgc
    36601 tagttacatt tatagttgca aaaatattta agtttagttt agaagaagct attcttgcat
    36661 ctaatgcaaa tattggtggt cctacaacag cagcagctat ggcaatatca aaaggatggt
    36721 ctaagctagt agggccaata cttatagttg gaacatttgg atacatagtc ggtaattatt
    36781 ttggtctttt agtaggaaat attttgatat aattttacgt gaaaaaaagt tgttttttag
    36841 aaagattttt atttaattgg aggttgttat gattttcgat ggacattcag atacttgggc
    36901 agatgtaaca ataagaaggc ttaacgggga aaaagatgta tttagaagaa tccatcttga
    36961 taattttaaa agagcaaata tagaaggagc tatttttgta atatggatag acccaccata
    37021 tgataaagag cctgaaaaaa gagttaaaca aattataagc tctataaaag atgaaattga
    37081 agatatgaaa gatgttgtaa acatagtaaa aaaatattca gatatagata aaggtataag
    37141 tgaaaacaaa ataaatattt taattggtct tgaaggatta agccatattg gaagaaatat
    37201 agatttacta gattattttt ataaagaagt tggtgcaaga catgcatcat taacttggaa
    37261 tgaacagaat gaacttgcta caggatgttt aggggatgaa aatcgtggtt taactgaaat
    37321 aggtaaaaaa gcagtcaata aaatggaaaa attgggcatg atagtagatg tgtcacattt
    37381 aaatgaaaaa tcattttggg atttaatgtc tgttgcaagt aagccagtta tagcttctca
    37441 ttctaatgct aggagtttat gtgatgctaa aagaaatctt acagataatc aattaaaaga
    37501 aatttcaaat acaggtggat tagttggaat aaatagtttt agaggattta ctaatagtaa
    37561 tcctaagaag caagatttaa tacatttggt tgaccatata gactatatgg taaatcttat
    37621 aggtatagaa catgtatctt ttggttttga ttttagtgat tacttaggga aagagactat
    37681 aggttctttt actggaaatg ctagtgtgcc aagtgtagat ggtcttgaaa gcacacatac
    37741 agcacagaac atagtagtag agcttgaaaa tagaggttat aaaaagaaag atatagagtt
    37801 aatctctagt ggtaattttt ataggctctt taaagatata ctaaaataat ataataaaga
    37861 acttaaattt ataaaatagt atatttatac aaataatatc tacatattta aattggcgtt
    37921 cataatttat taaattaaat ttta
//
LOCUS       DAMJVI010000030        37335 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.031, whole genome shotgun sequence.
ACCESSION   DAMJVI010000030 DAMJVI010000000
VERSION     DAMJVI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 37335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37335
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.031"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..233
                     /locus_tag="PC545_001582"
     CDS             <1..233
                     /locus_tag="PC545_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2456354.1"
                     /translation="QLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREW
                     IYPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            665..919
                     /locus_tag="PC545_001583"
     CDS             665..919
                     /locus_tag="PC545_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456355.1"
                     /translation="MENKDIKLTICIFAFVIGIIVLFCCIGLGQFNMDCIIKQNGGSI
                     ATEQYNIYLEQSISQYRNFGSILVLLGGIGILFDKSVRKY"
     gene            955..1278
                     /locus_tag="PC545_001584"
     CDS             955..1278
                     /locus_tag="PC545_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456356.1"
                     /translation="MARDVFSVKMTPLEAQKMVEKNLEADLVSVDIYNLENNKSIIVT
                     IFEKYYARSNSDAGLVVICENTTGKTLVKITSTGSAIGLFQIDWGTGNNLIKRVKKIF
                     IDYII"
     gene            1552..1704
                     /locus_tag="PC545_001585"
     CDS             1552..1704
                     /locus_tag="PC545_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456357.1"
                     /translation="MDNFLFNVLASVTASYIVYLISKLIKKVKSHSTAKSDLKIDINF
                     KYHRKK"
     gene            1898..2821
                     /locus_tag="PC545_001586"
     CDS             1898..2821
                     /locus_tag="PC545_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDF2456358.1"
                     /translation="MVGAFVLPNYAIILSEMSNLWGNFIMWILVPIITIVLLIIAVSS
                     MQYILVMIAFLLIIYSFIEKKIVMGLVSVLFFTYSIYLCATWEDKSLIADNKVETVKA
                     QRETVEREKEMERRRIQEEVDKERYIEKHGMEISENDLKVKLEALVPQEYKGKKYELK
                     VGKFKRYSMYFDLTVQNEKFSNSEECKKFVKEIANDLKKIKISKAYFKFHSKDDGGIY
                     NSVYIDYFRNIQNNVDNVENLEFNEFELKTEEEEKREQEKIEQEKNSYNNYIQNRVVD
                     PLDRIKKLKELLDSGAITQEEYNKKKKELLE"
     repeat_region   3088..3768
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3088..3115
                     /rpt_unit_seq="gttttagattaactatatggaatgtaaa"
     gene            3924..4112
                     /locus_tag="PC545_001587"
     CDS             3924..4112
                     /locus_tag="PC545_001587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456359.1"
                     /translation="MNKIATKSRAEYMKNRRKDKRGFSVLLDKEKLDKFDEVLEEKNL
                     TKKEWLEEKIDEELEQKE"
     gene            4232..5046
                     /locus_tag="PC545_001588"
                     /pseudo
     CDS             4232..5046
                     /locus_tag="PC545_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894376.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin Bro"
     gene            5098..5259
                     /locus_tag="PC545_001589"
     CDS             5098..5259
                     /locus_tag="PC545_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456360.1"
                     /translation="MCENLLDNMYIEKRKEEYRIKLLKIRETDIDIYNKLESIVYKLS
                     EKKLEKNNK"
     gene            complement(5588..6052)
                     /locus_tag="PC545_001590"
     CDS             complement(5588..6052)
                     /locus_tag="PC545_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456361.1"
                     /translation="MDFGEVKSELSDKDLKDNEEALNKYKKYYLKVYKYYSEMDSIKS
                     IQDIEIEQSRLKGQLQKNSNPIFALYISIMAVVFSTTINLTFDLSDITNYFIRMIMTL
                     LFIVLLIFLVNKGILPIAIPYRNNIQFYTTALDVLEDMKKKILRERKYKKRR"
     gene            complement(6188..6505)
                     /locus_tag="PC545_001591"
     CDS             complement(6188..6505)
                     /locus_tag="PC545_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456362.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLEVLYRYVTGLEYIAIGLFTRRLDITIIAIILVAIITTALYYLIRKKY
                     ITI"
     gene            complement(6788..7084)
                     /locus_tag="PC545_001592"
     CDS             complement(6788..7084)
                     /locus_tag="PC545_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456363.1"
                     /translation="MYFKTYILKREVNNKQLEITRSFDLDNLICTATEILKDNEIDLI
                     ITNEVNNVVWRNEKSTPPARECLVIAIEVYWWYTSIFLLYHINYVIKKSYLNRF"
     gene            complement(7103..7516)
                     /locus_tag="PC545_001593"
     CDS             complement(7103..7516)
                     /locus_tag="PC545_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456364.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLVIYCILKDKRFLISSFLLIVI
                     AIFNFCNAFSRKGIVEELHDSADERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLLVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(7500..7733)
                     /locus_tag="PC545_001594"
     CDS             complement(7500..7733)
                     /locus_tag="PC545_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456365.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            7952..8326
                     /locus_tag="PC545_001595"
     CDS             7952..8326
                     /locus_tag="PC545_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2456366.1"
                     /translation="MKISKLPEAELKVMRYICEANKVLTSRELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNILVKEKKYLRFETKEFLKNIHKNSISSLMSALH
                     GEDDEVDEDKLSELEEYFKNLK"
     gene            8938..9630
                     /locus_tag="PC545_001596"
     CDS             8938..9630
                     /locus_tag="PC545_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456367.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIIGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            9826..11886
                     /locus_tag="PC545_001597"
     CDS             9826..11886
                     /locus_tag="PC545_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2456368.1"
                     /translation="MEKYRVLKLVSLIIISISFPLTILLVTIGLAEPKYNIQKTSTKS
                     GIFDISDDFYDSNIFDEGTMASLLPKFKLSVGGKLTSKEKGKFQQEGKSIDLSSSDSE
                     YDKIMINEQASEEYLSYLKNIKVYVINTYTGVFYTNTSYASLYDFKQKTKSYCNVEII
                     NKIGKSSYIKTINGEKFELNNLSLELNEVDEGFEAYVSFPEEPTIEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLSIILYIKIKGEVLKRNNVFLLIYSKIPMEFNVILVS
                     ISIAILTIYALAVSYHIDVIALVSLSLLIVSNNIFFINVQLSYLENKMCIFKTSIIVR
                     VILWLIKTVREVSNAIKKVSLARKVIVIVIISLALIMAISTRSIGMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVALQGGKFYIEIDGDLFKANLEFQKYEEEI
                     TNME"
     gene            complement(12092..12415)
                     /locus_tag="PC545_001598"
     CDS             complement(12092..12415)
                     /locus_tag="PC545_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDF2456369.1"
                     /translation="MNNNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLYLSIANPKIISFMIVLLVAYKFKSIGISIITGVISIYL
                     FGIIL"
     gene            complement(12408..13109)
                     /locus_tag="PC545_001599"
     CDS             complement(12408..13109)
                     /locus_tag="PC545_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDF2456370.1"
                     /translation="MERTQQLKESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEESYE"
     gene            complement(13387..14772)
                     /locus_tag="PC545_001600"
     CDS             complement(13387..14772)
                     /locus_tag="PC545_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDF2456371.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALVEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIIKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTSDSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGIQLIPIK
                     QEDNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYREALSSALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPDGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESTEELKAGL
                     SVLKRLLEEKK"
     gene            complement(14880..15023)
                     /locus_tag="PC545_001601"
     CDS             complement(14880..15023)
                     /locus_tag="PC545_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456372.1"
                     /translation="MYDCDNQPKTPEEAVLYTRKRNFNIFKKTIPLYDKEGIKIIGRF
                     EIN"
     gene            complement(15218..15640)
                     /locus_tag="PC545_001602"
     CDS             complement(15218..15640)
                     /locus_tag="PC545_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDF2456373.1"
                     /translation="MNTFKTEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESTITRYELVHIDLENTKLIFIESESKRNIF
                     GKINSALVRVLFGKRQPEEFNKFIKKLEMELENNSVNL"
     gene            16118..19021
                     /locus_tag="PC545_001603"
     CDS             16118..19021
                     /locus_tag="PC545_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2456374.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISAILQKNKNISVSEITRELMTEGFNISRRVVEELLPKESLEM
                     DVVEESEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIIGESGVGKSLFS
                     RHMYEFAKQKKIVKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDTPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     IEYTEIRKYLKNAIFIPFNLEESSVKGSLLSDEYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSNEEIFSMSDLNSFVSDSLVKLVKELMNDIVEKNM
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEVKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNISTKTID
                     RVDLLMALEATRKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENLKETNNILAIVGTINPKIPGINFI
                     PYNKELFKNLDIYLSKSKQQDDRPVKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGAIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDAEFMNNANKCFDKDAFRRIIFDKFKHL"
     gene            19054..19500
                     /locus_tag="PC545_001604"
     CDS             19054..19500
                     /locus_tag="PC545_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2456375.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKETYINAVLERE
                     RTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLDVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNIKLLKNIKNASTKEEVAKLLDFIEI"
     gene            19623..19925
                     /locus_tag="PC545_001605"
     CDS             19623..19925
                     /locus_tag="PC545_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2456376.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            20106..21512
                     /locus_tag="PC545_001606"
     CDS             20106..21512
                     /locus_tag="PC545_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2456377.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRINTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGLSVVIYFVLWALFRKNKTSIL
                     DYLEKQALKNVEEEPVAV"
     gene            21856..22914
                     /locus_tag="PC545_001607"
     CDS             21856..22914
                     /locus_tag="PC545_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2456378.1"
                     /translation="MGDKMRASVLYSVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGKCDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEEHILKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIRVGDSVVVLGCGPIGQFVIQWAKVFGASKIIAVDIFDEKLELSKLLGANYILNS
                     KEVNVIKEIKKITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATECILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            23013..23699
                     /gene="araD"
                     /locus_tag="PC545_001608"
     CDS             23013..23699
                     /gene="araD"
                     /locus_tag="PC545_001608"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDF2456379.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNIVDGKLRPSSDTPTHLVLYKEFEELGGIVHTHSSWATI
                     WAQIGKSIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNIMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            23717..24364
                     /locus_tag="PC545_001609"
     CDS             23717..24364
                     /locus_tag="PC545_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDF2456380.1"
                     /translation="MLDSLKGKIVEIAIKAEHYGLCKEKTGNFSIRDKDTGYILITPS
                     GVGREELKVEHICVLDLDGNVIEVEKGIKPSSEVLMHIEIYKTRENINAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKSVSKLLIDTDACLLERHGL
                     TTVGNDLDEALLKSRYVEEVAEIYYRSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(24537..25904)
                     /locus_tag="PC545_001610"
     CDS             complement(24537..25904)
                     /locus_tag="PC545_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDF2456381.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILISFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(26094..26182)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            26610..28730
                     /locus_tag="PC545_001611"
     CDS             26610..28730
                     /locus_tag="PC545_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDF2456382.1"
                     /translation="MGKILVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVITWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKTIKRLWISSSTDKAIKDGFAKLRSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSKTCNKLLKSNIIANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNILEKDVLKVKGLKITSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPVDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQNKNQETFNNPFAEALAKLKNK"
     gene            28834..30087
                     /locus_tag="PC545_001612"
     CDS             28834..30087
                     /locus_tag="PC545_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HDF2456383.1"
                     /translation="MDLSGIFKYYCKECENTWNNSSVELFENIETYSKDSQKKREKEL
                     DKLLNTISVHLERYPSDAVLRKMWVKKGEVFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDDDLPIGDIMQAMRNVWISNALQLLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTNIDKNDKILFNNWLEKRLLGEHIKSKDYHESKVSKMIDYIESVYPREKFTEV
                     YESLLLIFKSQVNSLKQHGKENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEIE
                     FCIGYGFLLQISDDLQDIKEDLKYNHKTIITEMSKEGTLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVEKFIPYTIDYSLEIEEKIK
                     EKFKNIDVLNNENEYKEMIDIICAE"
     gene            complement(30240..31301)
                     /gene="ytvI"
                     /locus_tag="PC545_001613"
     CDS             complement(30240..31301)
                     /gene="ytvI"
                     /locus_tag="PC545_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HDF2456384.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIRLITNNTNLNRTLISIVILLIFYGLCIFLLVSFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALILGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIINFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
     gene            31575..32315
                     /locus_tag="PC545_001614"
     CDS             31575..32315
                     /locus_tag="PC545_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDF2456385.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKELN
                     IKYIFDYRSDEEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIEYKPKFKDIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YISVEAYLYGEFNLNKEEIRKLRNQYLE"
     gene            32327..34105
                     /locus_tag="PC545_001615"
     CDS             32327..34105
                     /locus_tag="PC545_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2456386.1"
                     /translation="MSLNITARQINIIKILLNSKDTISGIALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFDEVNLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRMLEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIKERKLLIKKLLLDILNENGLVLTDIEFKSVLNNILVMLS
                     RHKYNEKEFIENYIENYRSKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISNDKV
                     FEEFLYKHISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDLKIEINE
                     DEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKEKLEILK
                     IIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSEKEIRLIEKYIETGNIYLD
                     LDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKIATTEIG
                     NMVTIPHGAIGKIFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEVIFSKIY
                     KRVDSIAKVISICENSSFEKFVNLFN"
     gene            34272..35189
                     /gene="pfkB"
                     /locus_tag="PC545_001616"
     CDS             34272..35189
                     /gene="pfkB"
                     /locus_tag="PC545_001616"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDF2456387.1"
                     /translation="MIYTVTLNPSIDYIVKLDELKTGSTNRVKEEYVYPGGKGINVSR
                     ILKELGNDNTSLGFISGFTGEYIIRTLEEKELKTDFIKIKSGFSRINVKIKALKETEI
                     NGQGPNIDDEDIDTLYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKKF"
     gene            35226..37145
                     /locus_tag="PC545_001617"
     CDS             35226..37145
                     /locus_tag="PC545_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2456388.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAILAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSSSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIDTKENDANKYPKVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YTINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
BASE COUNT        14889 a         4033 c         6156 g        12257 t
ORIGIN      
        1 gtcaattaga atacaaggct aattggcatg gtagacaaat tgtaaaggtg ggtaaattct
       61 ttgcaagttc acaaatatgc aataaatgtg ggtataaaaa cgaggaagtg aaggacttaa
      121 atataagaga atggatttat cctagttgta atgaaactca cgatagagat ataaatgcaa
      181 gtataaatat attaaaagaa ggattaagac taataacaat tcaaaataaa taacgtatgt
      241 aagaaccgta ggaactacgg ggatagcctg tggagaatta gtaagacata tgttagtatg
      301 caaaattctg tgaagcagga aacctgtgac tttagtcatg ggaggttcag gctagtatta
      361 ctcattattg tattcaaaag ctaaagtgga aacctagtga atatatgaat ttagaagtta
      421 atgagagagc attagcagcc gactcaatac ttattaaaat tgaagatgaa gaagaaacaa
      481 tgaaagaagc tgaaagagag aaaaagagag gtagaagaag gtagaattta aagttaaaat
      541 agttacattg ctaatatatg aaaatgttac aaatttggaa atagatggat atatatgttg
      601 tatagtataa tgtagataat atatcaccaa gtaaaaaaca acaaataaat ctaaattatg
      661 aggggtggaa aataaagata ttaaacttac aatatgtata tttgcttttg taattggaat
      721 aatagtgtta ttttgctgta taggtctagg tcaatttaat atggattgta ttataaaaca
      781 aaatggtggg tcaatagcta ccgaacaata caacatatat ttagaacaat ctatttcaca
      841 atatagaaat tttggttcta tactggtttt actaggagga ataggaatac tgtttgataa
      901 aagtgtcaga aaatactaaa aaagtatatt tttatttagg atgtgatgaa tgatatggca
      961 agagatgtat ttagtgtaaa gatgacacca ctagaagcac agaaaatggt agaaaaaaat
     1021 ttggaagcag atttagtttc tgttgatatt tataacctag aaaataataa gtctataata
     1081 gttacaatct ttgaaaaata ttatgcaaga agcaatagtg atgcaggttt ggtagtgatt
     1141 tgtgaaaata ctacaggaaa aacacttgtt aaaattacct caactggtag tgcaattgga
     1201 ctatttcaga ttgattgggg aacaggtaat aatttgatta aaagagtaaa aaaaatattt
     1261 attgattata ttatttaatt attttatgta gatattctta ggttggtatg gaatttgtac
     1321 taacctatta agtaatcaat aaaactaaat aaacatagaa agcacttaca aatatgtagg
     1381 tgctttttat tgaaaaaata gaaaataaaa taaaaagtaa gatataggaa aaatatgtaa
     1441 gaattatatg ttataataat tgtagcaaga agatgtaatc tacaatttat agagtggagt
     1501 tcatgtatta aaaaattatc ctcccaacga taagaaggga ggtgaatata catggataat
     1561 tttttattta atgtgttagc tagtgtaaca gctagttaca tagtttattt gattagcaag
     1621 ttaatcaaaa aagtaaaaag ccactctacc gcaaagagtg acttaaagat tgatataaat
     1681 tttaaatatc atcgtaaaaa ataactttca gttatagaac ttcactctac cgcaaataga
     1741 ttgtagttct tcttgttttt attataccac aaattagaaa aaatattgca tataatattt
     1801 tttatagtca ataaaaatat gaaatttttt atacacaatg atgatgaatc taactagatg
     1861 aataaattta attgtaaata agaaagtact tacttaaatg gtaggtgctt ttgttttgcc
     1921 aaattatgct ataatattga gtgagatgag taatttgtgg gggaatttta taatgtggat
     1981 attagtacca ataataacaa tagtattact tataatagct gtttcttcta tgcaatatat
     2041 tttagttatg atagcttttt tgttgattat atattctttt atagaaaaga aaatagttat
     2101 gggtcttgtg tcagttcttt ttttcactta ttcaatttat ctttgtgcaa cttgggaaga
     2161 taaatccttg atagcagata ataaagttga gactgttaag gcacaaagag aaactgtaga
     2221 aagagaaaaa gaaatggaaa gaagaagaat acaagaagaa gtagacaaag aaagatatat
     2281 tgaaaaacat ggaatggaaa tatcagaaaa tgatttaaaa gttaagctag aggctttagt
     2341 acctcaagaa tataagggga agaaatatga attaaaagta ggaaaattta aaagatatag
     2401 tatgtatttt gatttaactg tacaaaatga aaaatttagt aattctgaag aatgtaaaaa
     2461 atttgtaaaa gaaattgcta atgatcttaa aaaaattaaa ataagcaagg cgtactttaa
     2521 gtttcattca aaagatgacg gcggtatata taattctgtt tatattgatt attttagaaa
     2581 catacaaaat aatgtagata atgttgaaaa tttagaattc aatgagtttg aacttaaaac
     2641 cgaagaagaa gagaagcgag aacaagagaa aatagaacaa gaaaaaaaca gttataataa
     2701 ctacattcaa aacagagtgg ttgacccact agatagaata aagaaactaa aagagttgct
     2761 agattcagga gcaattacac aggaagaata taataaaaag aaaaaagaat tattagaata
     2821 gataatatag taagcactta caagtatgta ggtgctttta aatttacaaa gtattccatt
     2881 ttaattttat agtttagatt ttatgatata ataaaaatat agaagttttg cagtgtgcga
     2941 tatttgttac aaagtagggc ttaatacttg aaatctaaga tgttgagggt gcgtgataag
     3001 tgttatcaat tgcactattg cccgctcact gcaattttaa gagtattgta tatatgtagg
     3061 tattggaaat gctaagttta ttttggggtt ttagattaac tatatggaat gtaaaactga
     3121 ctcgggagca tataataagt aatatcaaga atagttttag attaactata tggaatgtaa
     3181 aattcgtaaa ataacaattg tcaggaatat gagaagtttg ttttagatta actatatgga
     3241 atgtaaaatt ggactcaaaa tataagttaa aaaaattttt aagttttaga ttaactatat
     3301 ggaatgtaaa aatttttgac gctatgaaga aatttactac aaaatttgtt ttagattaac
     3361 tatatggaat gtaaaaactt ttttcatcct cccaaacctc ttgttcgctg tcgttttaga
     3421 ttaactatat ggaatgtaaa aaagaaaata agtttaaaaa aaggtttcat tattatatcg
     3481 ttttagatta actatatgga atgtaaaacc aattatgtac agtttagtta ctgtgttaag
     3541 tcttgtttta gattaactat atggaatgta aaatcctttt ataaatatct tctgacatgt
     3601 gtatatgctt gttttagatt aactatatgg aatgtaaatc ggctacaacg gtataaatta
     3661 aatttagcag ggtacatttt agattaacta tatggaatgt aaattgctgt gatttcaata
     3721 ataaaacaat taataattgt gttttagatt aactatgtgg attcaaaatt aaataaacaa
     3781 agaaagcact tacaaatatg taggtgcttt tattctgctc aaaattggtc ggttgggtaa
     3841 aataattaga aaaagttagt aaaaacctat tgactgtaac tcgttacaat attgttatta
     3901 atgtaacgag ttacagaaaa gaggtgaata aaatagcaac taaaagtagg gcagagtata
     3961 tgaaaaatcg tcgaaaagat aaaagaggtt ttagtgtact tttagacaaa gaaaagttag
     4021 ataaatttga tgaagtgtta gaggaaaaga atctaaccaa gaaagaatgg ctagaagaaa
     4081 aaatcgacga ggaactggaa caaaaggaat aaaaaataag ggtcactccc accgaccaaa
     4141 gtttgagtaa cccctatgac gtatactatc gtatatcaat tatagtatat gtcattcctt
     4201 aaaaaaatca attattaagg agtgtaatat tatgaaaaat gaattaatga tgtttgaagg
     4261 aaaagagatt gaagtatttg aatttgaagg aagaatttta ttcaactcaa aagatgtagt
     4321 aaactgttta gacataaaaa atgttaatga gaatataaga cttatgaatg aaaagcaagt
     4381 tgtaaagtta agaaattcgg atatcagtaa tactgacatc cgaaaactta ataatgctgg
     4441 agaaaatttt cttactgaaa gtggagtgta taaattaatc tttaaatcaa gaaaagaaga
     4501 agctgagaga ttccaaggct ggataagtga tgaagtttta ccaagcataa gacaaactgg
     4561 tgcatacata aataataatg caataccgaa aagttgagag aaaaagcaag tgagattgaa
     4621 aaattacagt tggtttataa cagtacatct atgttaaaag aattgctaga tgatgcaggc
     4681 tttgacaata aatcaaaact attaacagct aaaacattat ataagaaagc aggaattgat
     4741 ttaccaatag agataaacga agaagaacat tattttgata caaaacaaat agcatctaaa
     4801 ctgaaaatat attctaagag taataaacca gctcagatgg cagtttgtga gattattaaa
     4861 aagattgatt tagaagatgg tgaagttaaa ggtgtttggg aaattaatgg ttcttggact
     4921 ggtactgtaa ataaatatac aaagagtgta atagataagg tgagaacttg gatagaggaa
     4981 aataatagac ctaccaagat tgcaggtgag aagaagaatt attatgtatt ttacaaaatt
     5041 gagtaaattt atcagttgta ttaaataatt tagtttagtt ttggggggat taatacaatg
     5101 tgtgagaatt tacttgataa tatgtatatt gaaaagagaa aagaagaata tagaattaaa
     5161 cttttgaaaa taagagaaac agatatagat atatataata agttagaaag tatagtatat
     5221 aaactttctg agaaaaaatt agagaaaaat aataaataga catataaagc acttggatat
     5281 tctactgttt caagtgcttt gtttggtata aaatggtata atggagatag agaattatat
     5341 taacccaaaa atggagaaaa tagagaagat gatttagttg acgctttatc agtaggagta
     5401 cttgcaggta agacagaatc tctagtacta ttagttggaa atggaataag tgatactcaa
     5461 aaaagtttaa taaataaaag agaatttaga aatataactc aaattggtgg aaatggaaat
     5521 gagaaagcat ttagtgaagt agaaaactta gttaaataga ttagaaggta gcaactagag
     5581 ttggttgtta ccttcttttt ttatactttc tctctctaag tattttcttt ttcatatctt
     5641 ctaatacatc taatgcagta gtataaaatt gtatattatt tctataaggt atagctattg
     5701 gaagaatgcc cttgttaact aaaaaaataa gaagtacaat aaataaaagt gtcataatca
     5761 ttctaataaa ataattagtg atatctgata aatcaaatgt taagttaatt gttgtagaaa
     5821 atacaacagc cataatagat atatacaaag caaaaatggg gttactattt ttttgtaact
     5881 gtcctttaag cctagattgc tctatttcta tatcttgaat actttttatg gaatccattt
     5941 cactataata cttataaact tttaaataat attttttata cttgtttaaa gcttcttcat
     6001 tatctttaag gtctttgtca cttaactctg atttaacttc cccaaaatcc ataaaatccc
     6061 tcctacaatg ttatataata tactttaatt tatagcatat ttaagaactt atatcagcaa
     6121 tttgtcgaac gattttagct aaaaatagag tataatgata ggcttattat ttataagata
     6181 ttatttttta tatagttata tatttctttc taattagata atataatgct gttgttataa
     6241 tggctactaa tattattgct attattgtaa tatctaatct tcttgtaaat aagcctattg
     6301 ctatatactc aagacctgtt acatagcgat aaagtacttc tagtttaaca gtttttttct
     6361 tatctatatt tccatatatt gattctaatt tattaaaata ttttttacta caaaaagcag
     6421 ttttaggttt aattaaatat attattgtag ttcctattat aactattatt ccaataagta
     6481 taagattgat atttccaaaa tccatagcag atacctccta ttaaatttgc ttttcttaat
     6541 caaagtatat cagtaacaca agtacaaaga gcatctaaaa tattacaaat attaacagga
     6601 tagataaaac tatagtaaaa attgtaataa taaagcgaac gaaaattatc atactaaaac
     6661 ataagacatg ttataattgt attagataga agattaacat acaccaaagc actccttaca
     6721 ataaagagtg cttatttttt tgaaattcat caatatataa actatcaaga accttattca
     6781 acacacctta aaatcgattt aaatagctct ttttgataac ataatttata tgatataata
     6841 aaaaaataga agtgtaccac caatacactt ctatagctat aactaagcat tctcttgctg
     6901 ggggagtgct tttttcattt ctccaaacaa cattatttac ttcattagta attatcaaat
     6961 caatctcatt atctttcaaa atctctgttg cagtacaaat tagattatct aagtcaaaac
     7021 ttcgagttat ctctagttgt ttgttattta cttctctttt tagtatataa gttttaaaat
     7081 acatttatat caccccttgt atttactctt ttttctctaa aaatatattg ataagtaaat
     7141 atgcaacaaa taaaaaaatc tcaatgacac atagtgttat agctataact agaagtgatt
     7201 gatttttcca tgcactataa gctatgatga aaagaaatgt aaaagtaaat aatgtataat
     7261 tcataatttt aactaagata tgacttgttt tcattgttaa gtacaagtct ctttcatctg
     7321 cactatcgtg taattcttca acaatgcctt ttcttgaaaa agcattacaa aaattaaaga
     7381 ttgctattac aattaaaaga aatgatgaaa ttaggaatct tttatctttt aaaatgcaat
     7441 aaataactaa actagcacaa cataacatac aaactataat acccactata aaacttcgtt
     7501 tactctttac tttcatgttt tttatcctcc atttctttat tttcttttaa acaacatagt
     7561 tcttcaatcg ttgtaccaaa cacttgtgcc atacgatagg caagcataag agaagggcta
     7621 tattgctctt tttcaataga aataattgtt ctgtttgata catggactaa atcagcgagt
     7681 tgttgctgtg ttagacccat atttgttcga aattctcgta cttttgtttt cattccataa
     7741 cctctttcta tatgaagcta acttcatgtg aagttagctt cataatatcc taaaatgagt
     7801 aaaatgtcaa tctcattaga taagtattat aaaacctaaa agaataatat ggtaaaaatt
     7861 ggatggaatt atttacctta attctcttgt ttgtaaaact ttattgatat agaatttaac
     7921 atatacataa atcggcaaag gggcgcgaat tatgaaaatt agtaaattac cagaagcgga
     7981 attaaaagtt atgagatata tttgtgaagc taacaaggta ttaacttcta gggaattagt
     8041 tgctgcaatg gaagaaaaat atgagtggaa tgaatcaact acatttacag ttttgaaaag
     8101 attagagcga agggagtttt taagtacaga gaaaataggc aaacttacac attataatat
     8161 actagtaaaa gagaaaaaat atctaaggtt tgaaacaaag gaatttttga agaacataca
     8221 taaaaattca atttcaagtt taatgtcagc gttacatggt gaagacgacg aagtggatga
     8281 agataaatta agtgaactcg aagaatattt taagaatttg aaataagaaa atgacaaatt
     8341 gatacataat aaataaaatg aaggtatgaa attataactt tttctacctt cattttgttt
     8401 attattctga acatgttaag aaaatgaaat aagtttgtat tttagttata taaattttaa
     8461 tagaatatgt acagtatata gtagatagaa tgtataaaaa tagaatttaa ataagtagct
     8521 attattgcag tgaaatgaat gtttctaagt atatttgggt ggaagaatgg tggaagaaaa
     8581 ttagtgaaat gtaattcttc caccattctt ccacccaaaa aataaaaact gttcaataag
     8641 aattaatata catcaattca gctgaatgca atcaaaatac ttaaatgaaa gtaatctgaa
     8701 aacaacataa ttcaaatcaa tttattcaaa taaacctcaa tttatcttaa atcttaagaa
     8761 atatttaaga attaggataa tattttctta agatttatat tttattatat agttataagg
     8821 gaaagttaca aaaatgtaag ccaaagtaat ataacaattt agtcattgtt gagaaaggga
     8881 gtgaaagata ttgtataata tagataagta ttattataca atagaggaag tgaaaatatg
     8941 tacaatatat tagtagtaga cgatgataaa gaaattgttg attcgataga aatatatctt
     9001 aaatcagaag gatttaaaat atataaagca tatgatggaa tgaaagcttt ggaaataata
     9061 acagagaatg acattcatct aatactaatg gatataatga tgcctaaatt agatggaatc
     9121 aaagcaactg taaaaatacg agaagaaaga aatattccaa taattttaat ttcagcaaaa
     9181 agtgaagata cagataaaat aataggatta aatattggag ctgatgatta tataacaaag
     9241 ccatttaatc tattagagct tatagctagg gttaaatcaa acttaagacg atatgtaact
     9301 ttaggtaatt atgaagatac atgtaaagaa attctaaaaa gtggagcttt ggagttaaat
     9361 acactaacaa aagaagttaa aattgatggt gaaaacataa aaatgactcc aattgagtat
     9421 aaaataattg aacttctttt agaaaataaa ggacgagttt tctcaataga tgagatatac
     9481 gaaaaagtat ggaatgagga aagttttaat tcagaaaata cagtagcagt acatatcaga
     9541 cgaataagag aaaagattga aataaatcca aaagaaccaa gatttttaaa ggtggtgtgg
     9601 ggagttggtt ataagattga aaaattttag tggaaaaatt ataaaagaaa agagtttaat
     9661 atttaattgc attttgattt tagtcatttt tacatttaca caaatgttag gaattttaat
     9721 atgttttaat tttttctctg agagtttaat ttactctatg gggattatgc ttttcttaaa
     9781 ctttatttta tataaaatac tgacaatgta gatgaggtgg gcaacttgga gaaatataga
     9841 gtattaaagt tagtttcatt aatcataata tctatctcat ttccattaac tatattgttg
     9901 gtgacaatag gacttgctga gcctaaatac aatatacaaa aaacttctac aaagagtggg
     9961 atttttgata tatctgatga tttttatgat agtaatatat ttgacgaagg aacaatggca
    10021 agtctattac ccaagtttaa gctatctgta ggtggtaaat taactagtaa agaaaaaggt
    10081 aaattccaac aagaaggtaa aagtatagat ttaagtagta gtgattctga gtatgataaa
    10141 atcatgatta atgaacaagc ttctgaagaa tatttaagtt atctaaaaaa tataaaagtg
    10201 tatgttatta atacatacac aggagtattt tatacaaata cgtcatatgc tagtttatat
    10261 gattttaaac aaaaaactaa gagctattgt aatgtagaga taataaataa gataggaaag
    10321 agctcatata ttaaaacaat aaatggagaa aaatttgaac tgaataatct gagtctggaa
    10381 ctaaatgaag tagatgaagg ttttgaggca tatgtatctt ttccagaaga gccaactata
    10441 gaagatggaa tagtatatac taattttcaa atatttaaac aagctacaga aaaagtaaga
    10501 ttattttttg tagctagttt aagtttagca attatatgtt tattatctat aattttatat
    10561 atcaaaataa aaggtgaagt tttaaaaaga aataatgtat ttttactaat atacagtaag
    10621 atacctatgg aatttaatgt aatattagta tctatttcaa ttgcaatttt aactatatat
    10681 gctcttgctg tatcttatca tattgatgta attgcattag tttctttatc tttactgata
    10741 gtatcaaata atatattttt tataaatgtg caattgagct atttagaaaa taaaatgtgt
    10801 atatttaaga caagtattat agtaagagtg attttatggt taataaaaac tgtaagggag
    10861 gtcagtaacg ctataaagaa agtatcttta gcaaggaaag ttattgttat agtgataata
    10921 tctttagctt taatcatggc aattagcact cgaagtatag gtatgggatt actgttttct
    10981 atatgtattt ttttaggatt tacaatatat ataactaaaa gactttcata tcttagttac
    11041 gtaatagaag gtacagaaag aataaaaaag ggagaattag attataagat aaaaattact
    11101 gggaatgata attttacgtc tttagctgaa aatataaata atattggaga aggtcttgat
    11161 agagctatag aagagcaagt aaaaagtgaa agaatgaagt ctgaccttat aacaaatgta
    11221 tctcatgatt tgaaaactcc acttacttct ataataaatt atgtagattt aattaaaaaa
    11281 gaagaatcaa tacaaccaga gtatataaat gattatataa atgtacttga gtcaaagtca
    11341 aaaagactaa aattattgat agaagattta tttgaagcga gtagagtaag tagtggcaat
    11401 atagaattac aaattagcaa gattgattta gtgcaactat taagacaaag tataggtgaa
    11461 cttgaagaaa aattatctaa aaataattta tatttaaaat taaatgttcc aaatgataag
    11521 gtgtatatat gggcagatgg aagaagaatg tatagagtat ttgaaaatct tttatctaat
    11581 atagcaaaat attccttaga agaaactaga gtctatattg atgtatatga ctatggtgaa
    11641 aatgtaaagg taactatgaa aaatatatct tcttatgaat taaattttga cccaagtgag
    11701 attatggaaa gatttaaaag agctgatgaa tcaaggaata ctgaaggaag tggattaggg
    11761 cttgctattg caagagattt agtagcttta caaggtggaa aattttatat agaaatagat
    11821 ggagatttat ttaaagcaaa tttagagttt caaaaatatg aagaagaaat aacaaatatg
    11881 gaatgagata gtatttaagt atatatattt tttttactgt cgaaagcttt tattataata
    11941 tattagaata tttacagtag agaatgaaga gtaaaaacag agtaacttta agtcactctg
    12001 ttttttattt aaaatcaata atataacaac ttaaattaaa attgtacaca tagttttaaa
    12061 ataacttata caataaaaca ttatccaaat atcacaagat aattccaaat aaatatatag
    12121 atatcactcc tgtaataata gaaataccaa tagatttaaa cttatatgca acaagaagta
    12181 caatcataaa agatataatt ttagggttag ctatagataa atatagacta tctccattaa
    12241 aaaatacatc tttagcagtt aaggaagtaa gtattgctac aggtataaat ttcatgattt
    12301 ttttcaaaaa atcaggcatc tctttttttg aaaaaattaa cataggaagc actcttggta
    12361 gatatgtaac aatgtacatt ccaattataa taaaaaatat ataattatta ttcataagat
    12421 tcctcctttt tacagtaaga ttcttctata aagaaaccaa ttaaagatgc taaaattgat
    12481 gcaactatta ttactaaatt gcttttgaat aatgcatata aaataacaga aataatgatt
    12541 gatgatataa atactacaat ataaatctta ttttttattt gagaaactat taaagctata
    12601 aacatagaag tcaatatgaa gccagaaata gaactgtcta tagatatact ttctcctaaa
    12661 aatgctccaa taaaattagc taaaactgat gaaattaagc agaagaaatt aagataaata
    12721 gcatttctat cagaccaatt tccagcagta aatttttcat aattaactgc aaaagtttca
    12781 tctgttatag tatgacaaaa tggcaatata aattttggac ttttattttt tatgtacatg
    12841 ttcaaattag aactcattag aaaatgtctt aagttaacta taaatgtagt caaaacaata
    12901 gatgttacac ttgcaccagt agaaatcatt gaagctgcaa tgaattgaga acttcctcca
    12961 aatactaaag ttaccattag tgctatttga aaaggtgaaa gaccagattt ttgtaatagt
    13021 attcctccta ctatacctat aggtatataa cctaagaata taggaattga agcaataaag
    13081 ctttctttta attgttgagt tctttccaca ataaatcact ccttttgtat taaatgtatt
    13141 aaagtattag taaaacagaa aaatagatta attgtataat tgcaattata attaaaaata
    13201 aatttggatt aatatataaa atactataca acaaatagat aatcttaaac ggttattaaa
    13261 ttttatctat ttgttattat gatgtaataa tacatattaa tctctataag ttaattacta
    13321 tctggtgtaa ttttatttta acaacaaaaa aaaatacaca caattaaaat attgtgtgca
    13381 atacaattat tttttctctt ctaatagtct ttttaatacg ctcaaaccag ctttaagttc
    13441 ttctgtactt tctgtagcac atacagctat tcttattgca gaaattggtt tttcttttcc
    13501 aacagcaaaa cgttcagaag catagacttg tacaccttct ttaagtgctt gtatctcaaa
    13561 tttttctgcc attattccat caggtagaat caaccatcta aatatacatt cttcatttcc
    13621 caaaagatta tatccagata aatattgatt aataatcttg tttcttcttc ttgcagattt
    13681 acggcgtgca ttaattaatt tatcggcttc acctgatgta atcatgcgat aagcaatttc
    13741 tgttaaaaaa taagattgag atagattaat gttgtataat gcgcttgata atgcctctcg
    13801 atactgttta ggtgtagaga tatatgctag ccgtaaacta ggtgcaataa tttttgataa
    13861 actcgtaata taaattgttt gatttggcag ataggaagcc actggattta aatgtgtttc
    13921 attaagtaaa ctatgaatag catcctcaat aacaattaaa ttgtatttag atgcaatatt
    13981 tgcaatcatt tttcgtccat tttgtgacat tatatgtgta gttggatttt gataatctgg
    14041 tattatgtat agacctttaa tattttcatt tttacaagca tataatagtc cttcttcact
    14101 aatctcatta tcttcttgtt taattggaat tagttgaatc ccaagcattt tagctgctgt
    14161 ctttattcca ggataagtta atgggtctac acctattcta tctccatgtt gaaacaaacc
    14221 agctaaaata gcaacaattg cattttgtcc accacttgca ggaagtaagc tatctgatgt
    14281 agtttcaagc ccagctttat aaattaattt ggcaatagct tctttttgcc atttagcacc
    14341 atctctttgt ccatattgga atatagtttt aaaatctatt tccttaatta tgtgtttaaa
    14401 caaagtattc atttcttcta gcgtgaaatc tggattcatt gtgcccattt caataaatgt
    14461 aattttatta ttactggaca tcaagaatag atttgaacgt gtatcataag atacaaaagt
    14521 tccacttcct gtaactccac ttattagacc ttttttttca caaattttaa aagctctaga
    14581 gatagtactc acattaacat ctaagaaatc tgccaattca cgttggggtg gtaatttagt
    14641 acctggtaat aaaaatccat tgttgatatc ttgttctagt tgttctacaa gcgccttata
    14701 taatatttgc ccttttgaag gtctctttgg tttccagttc ataggataat tttcaaaaga
    14761 attaattggc ataagttttc acctcacatt attaatttga ttttttcttt atctaaaaca
    14821 ataaattcta aagatacaaa taaaaactga taggaaataa ttctatcagt ttttgtcttt
    14881 taattgattt caaatcttcc tataattttt ataccttctt tatcataaag agggattgtt
    14941 tttttaaata tattaaaatt tctttttctt gtatataaaa cagcttcttc tggtgtcttt
    15001 ggttggttat cacaatcgta caaatttctt ttttttaact aaaagcaata atacataaaa
    15061 ttattattgc taatgatact cttaatgtgg tatatagtca tagtgtcaca tgtctatcaa
    15121 tagttttttc agtaattatt gtaacgaata gtaggactca taaaatactt agaaatatgt
    15181 ttttagataa attttaaata tataatctaa cttatatcta taaattaaca ctattattct
    15241 ccaattccat ttctagtttt tttataaatt tattaaattc ttctggttgc ctttttccaa
    15301 acaatactct tacaagagct gaattgattt ttccaaatat attcctcttg ctctcacttt
    15361 caataaaaat aagttttgta ttttctaagt caatatgtac taattcatat ctagtaatag
    15421 tactttctct tttattgctt gtttttatct catatactct atttttttcg aaattagtta
    15481 tttcaacctt aaatctttta ttattttttt gctgtacaaa tgttcctact gcttttttct
    15541 cattaaaatt aggaaaattt agtcttgcag attttgtaaa tatattaaat atgtcttttg
    15601 cagaatattt taataaaact tcagttttaa atgtattcat atcgttttcc cttctgtata
    15661 taaatttaat atttataagt taatttttaa gttaaaatag tataatatgc catattaaat
    15721 catgttaaaa ctatttatat agctaaaatc ttacctatat aagtatgtat actatcatat
    15781 aaaaataaaa tcttaaaata gaaagggtta aaaacacagt ttattataat ttttattgtt
    15841 gagtagatac gtactattat atatgattga gcaagttttt caatcataaa tttataaagt
    15901 attttgcatt aaaatttaaa caaagaatta aattatttaa gattatgaat aactaataca
    15961 tttagtaaat tactaaatat acaaaatgta aatacttatc aaaatgtata aatacttatg
    16021 acatctagtt acttacaggg tttaagtaga aaaaaatatc aaagtaagta tggcatgata
    16081 gttgcttata tatttatcaa gaaaggagtt attaagcatg gagttgagag ataaacttgt
    16141 aaacatcata aatacagaaa ataaaaaaga tccattaaca gatgttcaaa tagcaaagtt
    16201 tttaagtaca actagagaga acataactaa tttgagaaaa gaacttaata taagtaattc
    16261 aaggcaaaga agatatcctt atttaaaatc agcaatttcg gcaatattac aaaagaataa
    16321 gaatatttct gtatcagaaa ttacaaggga attgatgaca gaaggcttta atatatctag
    16381 aagagttgtg gaggaacttt tacctaaaga aagtttagaa atggatgtag tagaggaatc
    16441 tgaagaagaa tcgaatgacc catttgaaac tctgataggt aataaaggga gtcttagaaa
    16501 tgctgtagag caggctaaat ctgctatatt gtatccacca aaagggttac caacgttgat
    16561 tattggggaa agtggagttg gtaagagttt attttctaga catatgtatg agtttgcaaa
    16621 gcaaaaaaag atagtaaaag aaagctccaa ttttgttgta tttaactgtg ctgattatag
    16681 tgacaatcca caattattat tatctttgtt atttggatat aaaaaaggtg catttacagg
    16741 tgctgaatat gatacaccag gtcttgtaga agaagctgat gaaggagttt tatttttgga
    16801 tgaaatacat agattgcctc caaaaggcca agaaatacta ttttctattt tagatagggg
    16861 aaagtttagg agattaggtg aaactaatgc tgaaagaaaa gtaagtatta tgttgattgg
    16921 ggcaactact gaaaatgtag agactaattt attattaaca ttcagaagaa ggataccaat
    16981 gctaataact ttgccaccat tacatgatag attactaaaa gagaaaatag accttatata
    17041 caatatcttt caacaagagt gtaatagaat aaatgcaaaa atatttgtag ataaaaatgt
    17101 gattgaaata cttgcactaa aaaagtttag tggaaatata ggacaattac aaaatatgat
    17161 tcaggttctt tgtgcaagag cttttatgaa gtttataaat tccaaagatg aagatagtga
    17221 atcgatagta gtagtagata taaatgaagt tttaaagctt aaagattctt ttaaagatgc
    17281 tgcctttcaa gagattgaat atacagagat aagaaaatat ttgaaaaatg ctatatttat
    17341 accatttaat ttagaagaat catcagtaaa aggaagtctt ttaagtgatg aatatatatt
    17401 accagaggac atatacaaaa caattgaaaa gaaatattat gacctaaaat ccctagacat
    17461 tgaagatata gatatagaga atattttatg gacttttatt ttaaatagat ttgctgactt
    17521 aaagtttaat ctcgatagta atgaagaaat tttttcaatg agtgatttaa atagttttgt
    17581 aagtgatagt ttagttaaac tggtaaaaga acttatgaat gatatagttg aaaaaaatat
    17641 gaatttagaa gtaaataaaa atatgttcaa atatctagca atacacttag aagaagctat
    17701 aaaaagaatt aagctaaatc aaaagataat aaatgtaaat ttagaaaaaa taaagattga
    17761 cttttcaaaa gaatatgaaa ttagtaaaag ttttgcaata aaaattgagg aatcaattgg
    17821 tattaagatt ccagatgatg aaattggatt tataacactc tacataaagt ctgctctaaa
    17881 aaatgaagtg aaaaaagata aagtagggtt aataataatt tctcatggac gtatagctac
    17941 agagactgta aatgtagtta aagaactttt aggtgtaaag tttcctgtgg ctatagatat
    18001 gcctttagat gaaaaaccaa taaatatata caataaggca gtagaactgt caaaaataat
    18061 agaccaaggg aaaggcatat tgtttttggt ggatattggt tcacttacaa acataggtca
    18121 aatagtaaat aagagaacca atataagcac taaaactatt gatagagtag acctcctaat
    18181 ggcactagaa gcaactagaa aagtttctat gggagaggaa gaacttgatg aaatattttt
    18241 ttctttgcta aaagatagga tgggatataa ctatgaatta gaaaaacaca tagataaatc
    18301 aaatgcaatt gtaacattat gtcttactgg agaaggaact gctaagtata taagtaaaac
    18361 gttggaagaa aagtatgata acactaaatg ttatcaaatg agtgcattgg atgaaaattt
    18421 attttgtaaa attgaaaatc taaaagagac aaacaatata ttagctattg ttgggacaat
    18481 aaaccctaag ataccaggta taaattttat accctataat aaagaacttt tcaaaaattt
    18541 ggatatctat ctatcaaaat caaaacaaca agatgacaga ccagttaaat cgtatgaaag
    18601 aatgcttgat gaggatttag tgatttttga accagatata tattttaaaa aggatttatt
    18661 agaatatgta tgttcaattc ttataaataa agaatatgta gaaaaagatt acttagattc
    18721 tgttttacat agagaagaaa tgcttccaac atattctaaa ggagctatag gtgtgcctca
    18781 tggagattct tctactgtaa atagtacaag gtttgttttt gttaaactaa agaatcctat
    18841 tgattggggt gtaggtaatg taaatttcat atttatgcca gtttttcagg ctaatgataa
    18901 agagattgtt aaaaatgttc ttgagatact aaaagatgca gaatttatga acaatgccaa
    18961 taagtgtttt gataaagatg cttttagaag gattatattt gataaattta aacatctgta
    19021 gccatagcaa aaaaattgag aggagttaga gtaatgaatg tagatttaat atcattgggt
    19081 ttagaatcta atgattatga aagtgttatt gaagaattag gaagtataat gtgtaaaaaa
    19141 gaatatgtaa aagaaacata cataaatgct gtcttggaaa gagaaaggac attaccaaca
    19201 ggtctagata ttggagaaat gtgtgttgct attccacata cagattcaaa gcatgtaaat
    19261 gagtctaatg tagctgtagg gatattgaaa aatcctgtaa agtttaattc aatgatagac
    19321 ccaaaagata gacttgatgt agaacttgta tttttattag cagtaaagaa cccagattca
    19381 caagtaaaac tgttaaagga tttaatgtca gtttttcaaa atataaagtt attaaaaaat
    19441 ataaaaaacg catcaacaaa agaagaagta gctaaattat tggattttat agaaatttaa
    19501 tgtaggagaa ttttataaat aaaaaattaa aatttaaaca atcaaagtaa gtattatttt
    19561 tttaataggt ttaatcttat ataaaataaa aatttagagt gaaaaattag gaggaaatta
    19621 ttatgaaaca atttaatgta ttatcagttt gtggttcagg gacagttaca tcatctatgg
    19681 tagcaggtaa attaaaagaa gtactaggta ataaggggat ttctattact acaacagaag
    19741 ctagaccaac agaagcacta aatttagcac aatcaggaag atttgacttt ataacattta
    19801 cgagtccact tcaaacaggc gattatggaa taccaactat aaatgcattt gcttgcctaa
    19861 ctggtatagg ggaagaggca ttctttgaag aagttttgac tgttattgaa gggatagata
    19921 agtaagtttt ggagacgtga atttatagta tttaaatcaa tgatttagat actataataa
    19981 gctaatatta actaaatatt tgtggaaaaa acattttata tgttgtcata aattaaaaaa
    20041 ttataaaaat agtcacttaa gtattaaatt aagcttataa atatttataa gtaggaggga
    20101 aaaagatgtt agaatttatg aaatctataa tcgatacatt tggttctgca attatagttc
    20161 caattataat attcataata gcaaagatat ttaaagtaac tactaagaaa tcgtttcttt
    20221 ctgctgtgta tgcaggagtt gctttacaag gttttacact gattttaaat tcatttacac
    20281 caataataac accagttatc aataggatgg ttgagagtac aggagtaaac ctaccagttt
    20341 ttgatgttgg ttggcaagca acatcattgg ttgcattttc aacaagtgca ggtatgatat
    20401 atttaggtct tggtatatta ttacaaacaa ttttattttt gataaaatgg acagacgtgt
    20461 ttcaaccatc agatttatgg aataactatt cttatatggt atggggagca atggttatag
    20521 gagtaacagg aaattttcct ttaggtatag catgtatggt tttattaaac ctatatagtt
    20581 tattaatatc agagttagtt gcaaagagat ggtctagtta ctatagatat cctaattgta
    20641 caataatagc tatgcataat gttgaagctt ctgtgtttgc agtatttgca gaccctatat
    20701 acaataaatt gggattaaat aaaataaaat taaatccaaa agaattagag aagaaattag
    20761 ggtttttagg tgaaccaatt actttaggtc tttttttagg tatgtttata ggtatattag
    20821 gtaatatgac gagaataaat actatggaag cttggggaga aataatgaaa gtaggtatat
    20881 ctacaagtgc agtgatggca attttcccta aagtagcaag tatgtttgct caagcatttg
    20941 cacctataac agaagcagct agaaagatta tgcaaaaagc tggaaaccgt gagtggtaca
    21001 tagcagtaaa tgatgctgtt gggtatggtg aacctgcaac tttaatatca ggtcttatat
    21061 taattccaat aatgcttgta atagctatgg tacttcctgg taataaggta ttaccagttg
    21121 tggatttatt ggcaattcca tatatggttc aaggtttagt tgccatacat aatggtaata
    21181 taccaaaagt attggtttca ggtataattt ggtttggatt aggattatat gtttgtacat
    21241 ctacagcacc attatttaca gatatggcta caaatatagg tgtagctata cctgcaggag
    21301 caatgttaat aacaagcttt aatatactag gaaaaccttt gatgggatta gttttctttg
    21361 catttctaag tgcaaatcca atttatatag gtctttcagt tgtaatttac tttgttctat
    21421 gggcattgtt tagaaagaac aaaacaagta ttcttgatta tttagaaaaa caagcactta
    21481 aaaatgtaga agaagagcct gtagcagttt aagtttataa aaatgaaata agaataatta
    21541 aaggagttta taattgattt tatataagta tcagataata cctgagaaat atttaaatga
    21601 aatatagact tctttttatt tgataatatt tgtaattata atgcattgta tctttatagt
    21661 tgagttttct gtataacctg ctatttaaaa catactcttt tatatttata cagtaatgtg
    21721 gtttaatttt ataaaatgta atctctactg gtaaaagact ttgtatataa ggcatttgcc
    21781 tgaataaacc atttatcttt tgtatggcat agatattgct atatagaagt atatagcatg
    21841 aaaggagata tttatatggg agataaaatg agagcatcag tattgtatag tgtaggtgat
    21901 gtcagatatg aaatggtaga tataccagaa ataacagata cgcaagtgct tgtaaatgta
    21961 aaatatgttg ggatatgtgg ttcagattta cctaggtcta tggtaagtgg tttgagtgga
    22021 aatacaaaat atccattgat tttaggacat gaattttctg gcgaagttgt taaaattgga
    22081 gaaaaagtaa agcatattaa tgtaggagat agagtagctg ttgcaccatt agtaccttgt
    22141 ggaaaatgtg attattgtaa tgaaggtaat tttggcttgt gtgatgatta caatataatt
    22201 ggaacaagag taaatggagc gtttgcagaa tatgtaagag ttccagaaga acatatttta
    22261 aaactaccag atacattgga ttatgaaact gctgccggaa ttgaaccagc aactatagct
    22321 tatcatggga tttcaaagtc taatattaga gtaggagata gtgtagttgt gttaggatgt
    22381 ggtccaatag gtcagtttgt tatacaatgg gcaaaggtgt ttggtgcatc taagattata
    22441 gcagttgata tttttgatga aaaattagaa ctttctaaac ttctaggagc aaactatata
    22501 cttaactcaa aagaagtaaa tgtaatcaaa gaaataaaaa aaataacaaa tggtggtgct
    22561 gatgttgtaa tagagactgc tggtagccga tttacacaag aacaatctct atttgtagca
    22621 aaaaagaggg gaaatatagt gtttgtaggt atatcacaca cagaattacc acttagtgca
    22681 gacgctactg agtgtatatt acgtggagaa cttaccttga aaggttcttg gaattcatat
    22741 acaagccctt atcctggaag agcatggact gcaacattgg attttatggg aaaaggtgac
    22801 attattttta aacccatgat ttcacataaa ataggtctta atgaagtggg tgacttttta
    22861 agtaaaatga gcaagagaga aattaatttc aataaaatcc tagtagaaat ataaaaattt
    22921 aataaaaaat tctaaaagca attaaatttt ataactatga cattaaataa aataaaaata
    22981 attttcttaa ataaaatata ggaggaagaa aagtgttaga aaatttaaaa gaagaagtat
    23041 taaaagcaaa cttagaatta cctaagaaaa agctaattac ttatacttgg ggaaatgtaa
    23101 gtggaataga tagagaaaaa ggattagtgg tgataaagcc aagtggtgta gagtatagta
    23161 atatgactgt agaagatatg gttgtagttg atttagaggg aaatatagtt gatggcaaat
    23221 taagaccttc ttcagatacc ccgactcatc tagttttata taaagagttt gaagaattgg
    23281 ggggaatagt acatacacat tcaagttggg caacaatttg ggctcaaata ggaaaatcta
    23341 taccatcttg tggtactact catgcagatt atttttatgg aagtatacca tgtaccagaa
    23401 aaatgactag agaagaaatt tgcaatgaat acgaaaagga aactggaaat gtaatcattg
    23461 aagaatttat gggaagaaat ccaattcatt gtccaggaat aattgtaaat gaccacggac
    23521 cttttacatg gggaaaagat gcaaatgagg ctgtacacaa tgcagtagtt ttagaagaag
    23581 tcgcaaagat ggcttattat acagagttaa tgagtcctga taatattatg gataaggttc
    23641 ttatgaataa gcatttttca agaaaacatg gaaaaaatgc atactatggt caaaaatgat
    23701 tatctggagg tgaattatgc tagactcttt aaaaggtaaa attgtggaaa tagcaattaa
    23761 agcagagcat tatggtttat gtaaagaaaa gactggtaat tttagtatta gagataagga
    23821 tactggatat atacttataa ccccatcagg tgtaggaaga gaagaattaa aagttgaaca
    23881 tatttgtgta ttggatttgg atggaaatgt aatagaagtt gagaaaggta ttaagccaag
    23941 tagtgaggta ttgatgcata ttgaaatata caaaacaaga gaaaatataa atgccatatc
    24001 acatactcat tctttatatg caacagcatt ttcagtagca aagaaaccaa ttataccaat
    24061 tgtatatgaa tcagttaatt atggcggtta tgtagatatt gctccatatg aaagaccagg
    24121 aactttaaaa ttagctaaga gtgtttcaaa attactgata gatacagatg catgcttatt
    24181 agaaagacat gggttgacaa ctgtaggtaa tgatttagac gaagcattat taaaatcaag
    24241 atatgtggaa gaggtagcag aaatatacta taggtcttta gtattaaatc aatttaaaga
    24301 acttgaagta gtagatgttg atgagtttaa atcttggaaa tacccagaag aaataaagtt
    24361 ttaatctaaa taattttcat tgaagtggtt aattaaaata tatgtattat ttatatcaat
    24421 tactatgttt tattatgtta attaccgtat tttgcaaata tatttattac attttttcaa
    24481 aaaaatatag aatccgaaag ccatataaaa ttgacttggg attctatact ttttatttat
    24541 gcatttttct ttatctgatt ttctggagaa tttatatgtt caaaatattt tttagtttca
    24601 gatactatta taggtgataa tgcaagtaaa gctataaggt taggtattgc cataagccca
    24661 tttacaatat cagcaattat ccaaacaact tctaacttta agaaagcacc taataacacc
    24721 atagccacaa atacagttct atacccattc atgcctttta caccaaacag aaattcaaaa
    24781 catctttctc cataataact ccatcccaaa attgtagtaa atgcaaataa tgttaaacta
    24841 actgtaagta atattggtcc aaatgtagga attgctccgt taaaagctgc ttgtgtcata
    24901 acagctccat ttaggtctga acaccacact cctgaaataa caagagagaa tccagtaaga
    24961 gtacagatta taatagtatc tataaaagta cctgtcatag atataagacc ttgttccgct
    25021 ggccattcag ttttagcagc agcagcagct attggagcac tacctagacc agactcattt
    25081 gaaaatacac cacgagcgat accatttcta attgccagtg acatagttgc tcctaaaaaa
    25141 ccaccagtag cagcagtagg tgtaaatgca cttttaatta ttaacataaa agtttcaggg
    25201 attttattcc aaaaagatat taaaatgatt ccacagatta caacatatat aactgccata
    25261 aaaggtacta ttttagtagc tacattagat atacttttaa gtcctccaaa tacaaccaaa
    25321 gctacaaata ctgctaatac aacccctgta attctagggt ctatcccgaa actattattt
    25381 attgcatctg ttattgaatt tacttgagta aaagtaccga ttcctaaaag tgcaactagt
    25441 ataccactta tagcgaaaaa tattgcaaga ggtctccatt tttctcccat accatttact
    25501 atgtaataca ttggcccacc agacacttgg ccatttttat ctttagttct gtattttatt
    25561 gcaagaacac cttctgaata cttagtagcc attccaaaga atgctgctat ccacatccaa
    25621 aaaagtgcac caggtccacc agctttgata gctgtagcaa caccaacaat attaccagta
    25681 ccaactgtag cagctagagc agtacataag gctgcaaaac ttgaaacatc accactacct
    25741 ttgttttcag cagagaatat taacttaaga gctcttggca gtttagttat ttgaacaact
    25801 ccaagcttaa cagtaagtag tataccagta ccaactaata gtaccagtag gggtggtccc
    25861 caaataaaag catcaacttt gtttaaaata tcaattagat tcataaaaac actccctcat
    25921 tttttttgct ttacataatc aatagaattt ttaatgaagt aattgacagg aaataagtat
    25981 aatttattaa taatttctag tgtaataaaa aaagctaaat ctaaaaaaga ttcagctctg
    26041 gatgtacact aattagcaac caaaataaaa gtattagtaa atactgtttt tggtagataa
    26101 tccctgtcct tttgcctgag agattgagca taaaagcctt gccccttcgg cgcccataat
    26161 gggtctctcc agagttccgt aaattttaca gtcctacttt taaaagtacc tgagagtgtt
    26221 actccttcgg tgtgctatat agcaactctt ctataaaatc ttaaccggat attgatttgt
    26281 catagcttat tgtattaata atattaatat aagtttttat gttttgtcaa ctaaaaaatt
    26341 aaaaaacaaa gaaaaaatat aaaaatgtaa gcctatttac gaattataaa cttagaaaga
    26401 tgagtaatat acgtgaaaat gaattttgta atacacatac aaatgaagtg taaaataagt
    26461 tctaaataaa aaaataataa aatcttaaat aattaattag atatgattaa attatggtaa
    26521 tattatagtt acgatattaa gaaaagttct atttagttag attttaagcg taatatgaga
    26581 ttatgtatag agaaaggatg tatttttaga tgggaaaaat attagtgcta gcagaaaaac
    26641 cttctgttgg aagagatata gcaaaagttt tagggtcaaa aaatgaaaaa aatggttata
    26701 tagaggggcc aaagtatgtg ataacttggg cactaggtca tttggttaca cttgctgacc
    26761 ccgaaagtta tggagaaaga tataaatcat ggagcttaga agacctgcca atacttccca
    26821 agcaccttaa gactgttgta ataaaaaaga gtggaaagca gtttaataca gttaaatcgc
    26881 aaatgaatag aaatgatata gatgaaatag ttatagcaac agatgcaggt cgtgaaggag
    26941 aacttgtagc aaggtggata atagaaaaat cacaggtaaa aaaaactata aaacgtcttt
    27001 ggatttcttc aagtacagac aaagcaataa aagacggatt tgcaaaatta agaagtggaa
    27061 aagaatatga aaatttgtat tactcagcaa tagcacgagc agaagctgat tggattattg
    27121 gaataaatgc aaccagagct ttgacaacaa agtacaatgc acagctttct tgtggaagag
    27181 tacaaactcc tactcttgca atgttgctta aaagagaaga agaaataaga aatttcaaac
    27241 ctaaagagta ttatggatta gaattaattg ctacaaaggg aaattcagat ataaaattta
    27301 tttggaatga taaaaacaat aattcttcaa cttttagtaa agaaaagata gaatccacat
    27361 taaaaaaagt aaaaggtgta gacataaaaa tatctgaagt aaacaagtca tataagaaaa
    27421 aatattcacc tgcactctat gatttaacag aattacaaag agatgcaaat aaaatgtttg
    27481 gattttcagc aaaagaaact ttatctataa tgcaaaaact gtatgaacat cataaagtac
    27541 ttacttatcc tagaactgat tctagatatt taacatcaga tattgttgga acattaaaag
    27601 atagaataaa agctgtaaat attagtgatt attcaaagac ttgtaataaa ttattaaaaa
    27661 gcaatattat agctaataaa tcgtttgttg acaattcaaa ggtaacagac catcatgcaa
    27721 taataccaac agaacagaag gtattcatga gtgattttag tgacaaagag cgaaaaatat
    27781 ataatcttgt agtaaaaaga tttttagcag tactatctcc tccttttgaa tatgagcaga
    27841 caactataag tgcaactgta gaaggagaaa tatttaaggc taagggaaac aaagtaataa
    27901 atttaggttg gaaagaaagt tataaagata ttgaagatga agatgagtat gaaagtatac
    27961 caaatatttt agaaaaagat gtacttaagg tcaagggatt aaagataact tcaggaaaaa
    28021 caaatccacc accatactta aatgagggaa cattattgac tgctatggaa aagaatggtc
    28081 tagggacagt tgctacaaga gcagatataa tagaaaaatt attcaattca tttttaatgg
    28141 aaaagaaaaa taaagatata tatataacat caaaaggaag acaattatta gatttagttc
    28201 cagtagactt aaagacacca gaacttacag cttcatggga aaagaaatta ttggatattt
    28261 caaagggaaa acttagtaaa aatacattca taaatgacat gaaagattat tcaaaagata
    28321 ttgtactaga agtaaaaaat agtgataata aatttaaaca tgataactta actaaaaata
    28381 gatgtccaga atgtggtaaa tttatgttag aggtaaatgg taaaaaaggt aagatgttga
    28441 tttgtgaaga tagagagtgt aatactagaa aaactatatc acaagtaacc aattcaagat
    28501 gtcctaactg ccataaaaaa ttagaacttc gtggagaagg tgaggggaga atcttcactt
    28561 gtagttgcgg atacagagaa aagttgtcgt ctttcaacaa gagaaagcaa gaagaaaaac
    28621 agaaggcatc aaaaaaagat gttaatcaat atttaaaaaa tcaaaataaa aatcaagaga
    28681 catttaataa tccatttgca gaagcattag ctaagctaaa aaacaaataa ttttaaagtt
    28741 attattatac acaattataa ctttaactgt atattagtta tatgtactga tataatatag
    28801 tgtatagttt aggcatgaaa gtagggctgt taaatggatt taagtggcat atttaaatac
    28861 tattgtaaag agtgtgaaaa tacatggaat aattcgagtg ttgaattatt tgagaatata
    28921 gaaacgtata gtaaagattc acaaaaaaag agggaaaaag aattagataa attgctaaat
    28981 acaatatcag ttcatttaga gaggtatcca agtgatgctg tattgagaaa aatgtgggta
    29041 aaaaagggcg aggttttctt acaaaagaca ttggaaaaag aaaatatttt taagttagaa
    29101 aaaatggatg tagaggatag aaaaaaattt ttagatataa caaaacagtt tattagagat
    29161 gctagaaaat ttgatgatga tttacctata ggtgatatta tgcaagctat gaggaatgta
    29221 tggatttcaa atgcattaca attattattt ggtaaagaag tatattattc aaaagctaac
    29281 tttgcatata gtatgttata tccatatact gataattatt tagacaatac aaatatagat
    29341 aaaaatgata agattttatt taataactgg ttagaaaaaa ggctcctggg agaacacatt
    29401 aaatctaagg attatcatga aagtaaagta tctaaaatga tagattatat tgaaagtgta
    29461 taccctagag aaaagtttac agaagtttat gaatcgttat tattaatatt taaaagtcaa
    29521 gtaaatagtt taaaacaaca tggtaaggaa aatcatttgt gtaaagaaga tttattatcc
    29581 atttctattg aaaaaggagg ttcatccgtt ttagtagatg gatatttaat aagtggattg
    29641 atgacaaagg aagaaataga gttttgtata ggatatggat ttttattaca aatatctgat
    29701 gatttacaag atataaaaga agatttaaaa tacaaccata aaactattat tacagagatg
    29761 tcaaaagagg gtactttaga taaagttgta aataaactaa taaattttac tattgagtta
    29821 atagatagtt ttaaaattaa taataaaaat aaatctgtaa taactatgat aaagaatgat
    29881 tgcttaatgt taattttatt ttctgtagtt tataatgctg aatttttttc tgtaggatat
    29941 ataaaagaag tagagaaatt tattccatat acaatagatt attcattaga gattgaagaa
    30001 aaaataaaag aaaaatttaa aaatatagat gttttaaata atgaaaatga atataaagaa
    30061 atgattgata ttatttgtgc agagtagaaa aaaagggagt gtcttagaat agacttttta
    30121 gtttatgagg cactcttttt gtataaaatc aaatgtattt tcatcatttt aataattaaa
    30181 aagagcttat ccctatttga gaaagccctt tttaatttaa taaaaatgtc aaggttaaac
    30241 tatttaaatg cagtaataat cccattatca tgtaagtttt ttatgattgt agctgcgata
    30301 gggaatccaa ataatccagc tattccaaat agttgagctc caacaaacat acacattaat
    30361 gttattaaag gatataggcc aatttggtgt cctactacct ttggttctaa tatttgtcta
    30421 acaacggtta taattatgta tagtattaaa agtcctattg ctaaattaat atttccatta
    30481 atgagtgaga taatcatcca aggtgtcaaa atacttcctg tacccaagac tggcaaaata
    30541 tcaacaagtg ctatgatgac agatatagta aatgcatttt caacctttag gatagtaagt
    30601 ccaataagta gttctatgaa tgtcactgat agtagtattg cataggcttt tagtagtttt
    30661 aaaagagcat taatactgct attttttata tttattatca taaattgact tttttctgga
    30721 aattgtttta agataaagtt tattattttt tgatagtcaa aagtaaagaa aaaagaagca
    30781 attaaagtaa taataagttt taacataaat gcaggtgttt tacttgcaat actagcaata
    30841 gcattgagtg catttgttga aatagaagct actaaagaca tcatagattg acttatatta
    30901 tcccaaccaa gtattaatgc taggttaaca ttaggtgtac tagcatctat ctttgaaaat
    30961 aaagtattta gcgttggttg tatacttgat ttatatattt caggaagtct aaaaaataca
    31021 tcacttacac ttgcaaatat cttcacacca aagctaacta ataaaaatat acacaaccca
    31081 taaaaaatta aaagtattac tatagaaata agtgtacgat ttaaatttgt attatttgta
    31141 ataagtctta taataggtct taagatggat gcaatgataa aactgattac aaaaggcatt
    31201 aataatggca ataagaattt taatacaata tatacaatac ttgttattaa aactacatat
    31261 gtaaatttaa ttataaaatt cttttgcttt tgaatatcca taatataaaa cctccaatgt
    31321 atttgatatt attctaaata tggtcttttt tacagaaaat atactactgt atattgatta
    31381 taaactataa agcttatgta aagtaaataa attatgtaaa atttaggtta attttgtata
    31441 aagagatact tttatatttt acaattaata accaaatact aaaaaatatt ttaaataaag
    31501 aacttaaaat tgtgataaaa ttaatttata gatattaaaa aaagtataat atatggtttt
    31561 taagaggtgc taagatgaaa aattttagag atttaggtgg gaacaaaact gaggatggca
    31621 gaactgtaaa gaagggatta ttttataggt ctgcaaaatt atcaaatcta agtgaaaatg
    31681 atataaagat tttaaaagag ttaaatatta aatatatatt tgattataga agtgatgaag
    31741 aagctcgtaa acatccatca acaataatat caaatataaa aaatataaga ataccagcaa
    31801 tgagagagct agaagaatca ggaggaagtt ttggttctat tgaagatatg attgatggat
    31861 tgtttgaaaa agatggtgca tttaatatgt taaataatag ttactataat ttgcctataa
    31921 ataatccgtc atataaaaaa ttagtagaac ttataagaga ctattctaat ttaccaatat
    31981 taaatcattg tacagctggt aaggatagaa ctggtgtggg gagtgctata attcttatga
    32041 ttttaggggt atcgagagag aatataatga aagattatct aaaaagtaat gattttgcag
    32101 ataaagagat agaaagattt atagaatata agcctaaatt taaggacatt ccaaaagaaa
    32161 atttgaaata tatatttggt gtaaatgaag agtatatgaa aactgcattt agaagaattg
    32221 atgaagaata cattagtgta gaagcatatt tatatggaga atttaattta aataaagaag
    32281 aaataagaaa attaagaaat caatatctag agtaaaaggt gagtttatga gtttaaatat
    32341 aacagcaaga cagataaata taataaaaat attgttaaat tcaaaagata caattagtgg
    32401 tattgcctta agtcaagaga taggatgttc ttcaaaaact atacaaaatg aaataaagga
    32461 tattaataaa caacttagaa atggtaaaat tttgtcaata agaggagttg gttataaact
    32521 agaaggaagt tttgatgagg taaatctaaa tagcaatctc tatgatgatg tagatagagt
    32581 agaatatata atcagaaaaa tattaacctt aagtaatgaa gaaaaaaata ctataaaact
    32641 tgaagattta gccgattcta tgtatgtaag tgtttcaaca gtaaaaaatg atttaaaaga
    32701 agtaaaaaga atgttggaaa agtattcaat atcagttaaa tctaagcata aacaaggtat
    32761 atgtgtttta gaaagtgaag ataagatatt aaattgtata gttgacttaa gcaataagag
    32821 agataatcaa ttgagtttaa atgatttttt aaatgatgat ataaaggaaa gaaaactatt
    32881 aataaaaaaa ttattattag acatattaaa tgaaaatgga ttagtattaa ctgatataga
    32941 atttaagagt gtgttaaata atatattagt tatgttaagc agacataagt ataatgaaaa
    33001 agaatttata gagaattata tagaaaatta taggagcaaa agaagctcaa ttttagaaaa
    33061 tgaacaaaac aaagaaatta taataaattc aataaaaaca ttttgtaaaa atttaaaatt
    33121 agctacatca atagatataa gtaatgataa ggtatttgaa gagtttttat ataagcatat
    33181 tagtagttta tgtaaaaaaa taaagttagg tataaatcaa tcacctataa tggctcaaga
    33241 tataaaaata aaatatccat ttgcttttga actagctaat atagctaaaa agacaataga
    33301 aaaagatttg aagatagaaa ttaatgaaga tgaagttgca aacatagctc tacatatagg
    33361 aggagcagtt gaaagagcat cttataataa taaagataag gttttcaaaa ctataatagt
    33421 ttgtacttca ggcataggca catcaatgtt aataaaagct aaattagaga atatatttaa
    33481 ggaaaaatta gaaatactaa agattattcc ttcatactta gttgaatata taaaagttat
    33541 agatgttgat tttgtaatat ctactgttcc tattgaagta ggagatgtgc cagtaataaa
    33601 tatatctcca attttaagtg agaaagaaat tagattaatt gaaaaatata tagaaacagg
    33661 aaatatatac ctagatttag atataaaaag cctgtttgat agtgaattgt tttttactga
    33721 tttggtgttt aaaaggaaag aagaagttat agattttatg gcaagtaaac ttgtacaaaa
    33781 gggctatata gatgaggata tgaaaaaatc ttactttgag agagaaaaaa tagcaacaac
    33841 agaaataggg aatatggtaa ctataccaca tggagctatt ggaaaaattt ttgataataa
    33901 aatagcaatt ggtatattaa agaagcctat ttcttgggaa atatcagatg ttcgtttaat
    33961 aataatgtta gcccttgata aagataaaat attagattat gaagtaatat tttcaaaaat
    34021 atataaaaga gtagattcta tagcaaaagt tataagtata tgtgaaaata gtagttttga
    34081 aaaatttgta aatttattta attagatggg tagtattgta tatacacctg taaatttgga
    34141 taatataaaa ctaaataaga taaaaacgat aaatttctct tttaattaag agaaattttt
    34201 tgactttcaa ggatattaat ataaagttaa gatataaaca taaagcaaat aaataaaaaa
    34261 gaggtggcaa tatgatttat acagtgacat taaatccatc aatagattat atagttaagc
    34321 tagatgagct aaagacagga agtacaaatc gtgtaaaaga agaatacgtt tatccaggag
    34381 gaaaaggtat aaatgtatct cgcatactta aggaacttgg aaatgataat acatctttag
    34441 gttttataag tggttttact ggagaatata taataagaac attggaagaa aaagaattga
    34501 agacagattt tataaagata aaaagtggtt tttcaagaat aaatgtaaaa ataaaggcgt
    34561 taaaggaaac tgagattaat ggacaaggtc caaatataga tgatgaagac attgatactt
    34621 tatataaaaa acttgataag ctaaatcaag atgatatttt aatattggca ggaagtatac
    34681 catctacttt agatgaaaaa ctatacgaaa atataatggc tagattagaa aaaaagaata
    34741 taaaagttgt agtagatgca actaaaaatc ttcttcttaa tgttttaaaa tacaaacctt
    34801 ttctaattaa gcctaataac gacgaattag aagaactatt tggtgttaaa ttaaattcaa
    34861 ttgaagacat ggtaaaatat gctagaaggt taaaggaaat gggagctata aatgtcttgg
    34921 tatcaatggg aaaagatgga gcattgttga ttacagagga agatgaagtg cttataagtg
    34981 atgttccaaa aggaaaagta aaaaattctg taggagcagg tgattcaatg gtagcaggat
    35041 ttatatctgg gtatttgaat actgggaaat atgattatgc tcttaaattg ggagcagctt
    35101 caggtagtgc aactgctttt tcatatgact tagccaaaag agaatacatt gataaattgg
    35161 taaatgaaat atctgtaaag aagttttaaa atattaaaaa atatatagaa aggtaattaa
    35221 tatttatgaa aattatagat ttattagatg aaaagtcaat taagttaaat ttaaattcaa
    35281 aaaccaaatc agaagcaata gaagaattag tagatttggt cgcaaatagt ggaaatttaa
    35341 atgataaaga aaactacaaa aaagcaattt tggctcgtga agaaatgagt acaacaggaa
    35401 ttggagaagg tgtagctata cctcatgcta aaaattcttc agttactaaa gcttgtatag
    35461 cagcagcagt gtcaaaagaa ggtatagatt atgaatcgtt tgacggaagt ttatcaaatc
    35521 tattttttat gatagctgca ccagacggag ctaacaatac tcatcttgaa gtattatcaa
    35581 gattatctac aatacttatg gatgaagact ttagaaataa acttataaat tcaagctctg
    35641 aaaaagaatt taaggaaata atagataaaa aggaaagaga aaaattcagt gaagaatatc
    35701 aagatgagaa agtggctgaa aaaaatataa tagatactaa agagaatgat gctaataagt
    35761 atcctaaggt tttagcagta acagcatgtc caactggaat agcacataca tttatggcag
    35821 ctgaaagttt aaataaaatg gcagaaaaca aaggtgtatc aataaaagta gaaactaatg
    35881 gttcagcagg tgttaaaaat aagcttacaa aagatgaaat agaaaatgca acatgtataa
    35941 tagtggcagc agataaaaat gttgagatgg ctagatttaa tggcaaaaaa gtcataaaaa
    36001 ctaaggtggc tgatggtata cacaaagcgg aagaacttat agacaaggct gtaaatggag
    36061 atgctccaat ttaccatgga ggagatggaa gtcacaacga atcaaatgaa gaaagtgaaa
    36121 gtggatttag aaaagtatac aaacacttaa tgaatggtgt atctaatatg ttaccatttg
    36181 ttattggtgg agggatactt atagcgatag cctttttact tgatgattat actattaacc
    36241 cttcaaactt tggttcaaat acacctatag cagcattttt taaaggaata ggagataaag
    36301 cttttggatt tatgttacct gtacttgctg gatatatagc atactctata tctgatagac
    36361 cagcgtttgt tgtaggattt gtaggaggtg ctctagcagg tgatggtggt tcaggctttt
    36421 taggtgcact acttgcaggt tttatagcag gttatttagt agaagggctt aagaaaatat
    36481 ttagtgtact accagcttca ttggaaggaa taaaaccagt attgttatat cctttacttg
    36541 gaacactttt gatgggaatt ataatgacat ttttagtaat acctccagtt acagcaatta
    36601 ataatgcaat ggttggattt ttaaatggtt taggtggaac ttcaaaaata gttttaggat
    36661 tagtattagg tggaatgatg gctgtggata tgggtggacc tgtaaataaa gcagcatatg
    36721 tatttggagt tgcatcactt gaatcagggc agtttgaaat aatggcagca gtaatggctg
    36781 gtggtatggt tccgccactt gctatagctc ttgcaactac attttttaag aatagattta
    36841 ctaaagaaga aagagattct ggtaaagtta attatgtaat gggattatca tttgttactg
    36901 aaggagctat accttttgca gctggagacc cacttcatgt tataccagct tgtgttggag
    36961 gttcagcagt ggcaggagca cttagtatgt tgttcaacgc agctcttcgt gcacctcatg
    37021 gaggagtatt tgttatacca gtggttaccc atccatttgc atatatatta gctatagctg
    37081 taggtgcttt ggttggaatg atgcttttag cattacttaa aaaaccatta aatcaagaag
    37141 tatagcaatt aaaggatatt caagaactta aaatatgatg ttttataaaa attaatatat
    37201 tgctgaatta ttttaaaata aggattttat gaggctgtta tataacgatt tgtaattgtt
    37261 atcttgacag tcttttattt ttatataagt aaaataagtg tgttttgctt tatttgtatt
    37321 taaattgttt ttaag
//
LOCUS       DAMJVI010000031        35268 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.033, whole genome shotgun sequence.
ACCESSION   DAMJVI010000031 DAMJVI010000000
VERSION     DAMJVI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.033"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            158..271
                     /locus_tag="PC545_001618"
     CDS             158..271
                     /locus_tag="PC545_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2456389.1"
                     /translation="MIMGRHLWNPSYFVATVLENTEEQIGKYINNQKKQVK"
     gene            273..1391
                     /locus_tag="PC545_001619"
     CDS             273..1391
                     /locus_tag="PC545_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2456390.1"
                     /translation="MIVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIEVY
                     KNDKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNVLKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKIRDKQVPQGRILNAAISKEPSGR
                     YYVSLCCTDVDIEAFENTNNQIGLDLGIKEFCISSCGEFIENPKYLKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKNM
                     IRNSKLSRLISDVSWSEFIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKD
                     LNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            1542..2759
                     /locus_tag="PC545_001620"
     CDS             1542..2759
                     /locus_tag="PC545_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDF2456391.1"
                     /translation="MLIGLIAAFGLVLQKKKASDVIKGSLLAAFGIIIMETGTGMLVS
                     SIAPINGAFQSISGGAVTKGLSDVTFTASYGGIIGLAMFAGLAIHLMIARFTPIKTIF
                     LTGHMLYWFPFIFVASGVEAGLNGGVLITFAAVLSALYWSIMPWLLRKYVFDVIGDDS
                     FTLGHPSGCLALISGFVAKLVGNKEKSTEDINIPQSLSFFREISISGGMAVFLMFIVV
                     GIVIPGAFKDSNEFIGTYAIKQGLSFGAGLIIMLHGVRMLINQIMPAFQGISEKVVPN
                     ALPAFDCPILFNYKPNAVLIGFLVAMLTSTAMILIYNYFNVFNLLLIPLVITSFFECG
                     AAAVIGESQGGIRGAIIGTAIAAIFMVVLVGISAVIYSGTIQNWILIFGGNDLSLFGI
                     ISRFIGSLFGGIL"
     gene            2762..3502
                     /locus_tag="PC545_001621"
     CDS             2762..3502
                     /locus_tag="PC545_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2456392.1"
                     /translation="MKFKYIDEIRRLIDIIENEEKVNMEKAVQIFVEAIESKNAIFSF
                     GASHAGILTEELFYRAGGCVLINPVFAKSLMLDTAPITHTSKMERLEGYGTALADKTP
                     IKKGDVVIVHSVSGRNPVSIEFAMKAKENKAIVICITNLSYSKSVSSRHSSGKKLYEV
                     CDLVIDNHGQKGDACVEIDGLNQKVSPTSTVIATTVMNSIIAQATQELVNKGLKNPPI
                     FYSANIDGGDELNKKIFDEYKSVIHYEY"
     gene            3825..5135
                     /locus_tag="PC545_001622"
     CDS             3825..5135
                     /locus_tag="PC545_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDF2456393.1"
                     /translation="MKKENKMEGIKVGMLKKLCSLAMVAIMTTTLITGCSSGNGKDSK
                     KDGEKEKLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISA
                     GEGPDVGYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAA
                     NPGVLYYNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFG
                     NLNWNWYPYLWQAGGDIFNDDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEM
                     IESVFGPGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSS
                     AKNKELAYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKNVYRPL
                     VVGPHGVEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            5233..6150
                     /locus_tag="PC545_001623"
     CDS             5233..6150
                     /locus_tag="PC545_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2456394.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVAFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTVVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGSIWSLQVFDLIYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVRRDNSDF"
     gene            6166..6975
                     /locus_tag="PC545_001624"
     CDS             6166..6975
                     /locus_tag="PC545_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2456395.1"
                     /translation="MKTARRLLSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFFNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDTMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            7033..8781
                     /locus_tag="PC545_001625"
     CDS             7033..8781
                     /locus_tag="PC545_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HDF2456396.1"
                     /translation="MEISYVLKDCTFKHNKYSVVEAETWKDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTIDKSSAISWKGLGNRVRLALNMPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFEENNLDIGIDIFKSFGYEDEEKVCTIDVP
                     VKVRNVVLKPLDESKFFLDLWQHPSCLARMYKVELWSDIHFEIVDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDRYISIADKY
                     KMAKEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDEKDKVFKFINNTNDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVSTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIESLKKKINDKGGILTWFVCCFPEKPNSFLSSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     MENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNW
                     GYDKVKKYVLELLDRV"
     gene            9043..10065
                     /locus_tag="PC545_001626"
     CDS             9043..10065
                     /locus_tag="PC545_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HDF2456397.1"
                     /translation="MFGGINIKRIGIIIMFFLSISFIGFYLVIRDNSDTVKMQRGINI
                     GNALESPKDFPWDVKMSNKFFDDIKDAGFDTVRIPVRFSDYTSDSDNFKIDEEFFKKI
                     DKYVDYALDKDLIVVLDLHHFEEIMKEPRVHKEKFLKIWQQIANRYQKYDKKLVFELL
                     NEPKENLYSQLLNEYIEEAIKIIRKTNPKRTIIVGPYNFYQIDYLNELNIPKDSNIVV
                     SFHYYEPNDFAFQGNIYHKGFEHLSNITWEGTNEQMDYLKKRFDTVENWANKNKVKIF
                     LGEFGITKEAPEASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGTWDRDILS
                     ALIEKR"
     gene            10070..12376
                     /locus_tag="PC545_001627"
     CDS             10070..12376
                     /locus_tag="PC545_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDF2456398.1"
                     /translation="MKKNSKKILITLTIITNVIYILWRIFYTVPKEEGKFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDKKKVHIYICDDGNREEMCILAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPEVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLVFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKSKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYIAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEVEFRE
                     KSGNEMYIANMKAKIVNVVEVNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRINMNFQMDIKNTGKLRIVNFNYQYVLLNF
                     ENKSIYPKEIALEINENIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKKILDSKPSEKKEKSIDEFEPMEYI"
     gene            12348..13463
                     /locus_tag="PC545_001628"
     CDS             12348..13463
                     /locus_tag="PC545_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456399.1"
                     /translation="MSLNPWNIFRRDEMKTLKVFFCSTKYLILVFIFFINTTFISSAT
                     SDKILDLSFKKIETDLSSKITYEDTGVKIETDSSKSDKERYLYIYQNIKENWSMYNNF
                     YIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEVFLEGKNIIYSDKIKEGCIEVPGEF
                     EGKIYVNFNSLINEESNVVLDSNMLSNIVSWGITFIPSDEEHNIVIIKKISLLSEEKL
                     RFLNNIKIIGDEEVQIPVLGQSISQYEVLGLKSDSKIKYSLMGKQDNVSISQNGKLTL
                     SNKAKPGQIILQVNVDDKFKIGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     KINFMNNIITFVRILFVSLVIICFGIYLYWKKCSKTK"
     gene            13481..14899
                     /locus_tag="PC545_001629"
     CDS             13481..14899
                     /locus_tag="PC545_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HDF2456400.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYVFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMIVSIIMNYIFGLLVDRYRESKIKVKVFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELISTELTIPNIILPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRSPNLKEAYNYFKAMFGG
                     NGAIWSDYTYMFLKEYWVFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            14914..16122
                     /locus_tag="PC545_001630"
     CDS             14914..16122
                     /locus_tag="PC545_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456401.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNVCYSGKDTIQEIKNSIAE
                     KSSLKDTISSIDNTVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGVIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNETA
                     DNFLNQLKKYNVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKDNYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLDLMDGQSDKYFTYLLGNRPLVKIKNIENPD
                     GLKVLFVKDSLIVPTAAFFSSVCSSIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(16196..17584)
                     /locus_tag="PC545_001631"
     CDS             complement(16196..17584)
                     /locus_tag="PC545_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2456402.1"
                     /translation="MESSFLSQFLMITDFKTIFFIILLLGTFFIVRQFEKKKIKFSLR
                     TIYATVLGLILGIIIQAFAGFPNDTTQVTWLQEVSKWYSLFGSGFMDLLKMLVVPLVF
                     LSIIKVIINMKDNNLGTLTFKSLGMLLATTAIAAIVGIIVANIMKLGVGANMPTLVEN
                     QELKEINSLVDTLRGLLPSNPVMAMANGNIVATIIFATFIATSIKRLSKKHFETIKPF
                     IDFIEASYKIIVSVSMTVIKFMPYAVVALLANTITSQGISSIVSVLQFILALYISVAI
                     LFIIHLIFITLNGLNPITYIKNSTEALLLAFTSRSSLGTLPVTIESLVQNHGVDEGVS
                     SFTASLGANMGMNGCAGIYPALMAVTLANMAGVNMDMSFYVMLLIVITISSFGIAGLP
                     GTATMAVSVVISGVGLGSYFPLVGGILAIDPILDMGRTMINVNGAMVSSITVGKSLGK
                     IDKEVFSKKNIS"
     gene            17925..19259
                     /locus_tag="PC545_001632"
     CDS             17925..19259
                     /locus_tag="PC545_001632"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HDF2456403.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFNDADELLARTPEKLREAGIDLNIFREVVEVDSESKKIKVKNIKTDEIYED
                     YYDKLMIATGARSIMPPIKNIKLKNVSTLKSLYDGEYLKKLLSNEDNKRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEMTDVLEEEINRHNVNLHLDEMVVELC
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSNTNIKMLKNGAIVVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITEKQAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVILGGQV
                     AGFKDAVQRANVLAAAIFGKMTTNQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(19401..20318)
                     /locus_tag="PC545_001633"
     CDS             complement(19401..20318)
                     /locus_tag="PC545_001633"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDF2456404.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILIDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPVGIVDCIRSMR
                     NLVEYVPYTLKIIENPQGNFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKQYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKDSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDIP
                     SMYLENATNVFENTILGEDRLSLRLNFDE"
     gene            20526..21410
                     /locus_tag="PC545_001634"
     CDS             20526..21410
                     /locus_tag="PC545_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HDF2456405.1"
                     /translation="MKVLKKITILSLITLTLVGCSNGEDKKVVEGSKKIQNSKKDEQP
                     IIVHKDANILKDRYEPPKGFKRVDVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWAQGY
                     RISVKDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            21623..23464
                     /locus_tag="PC545_001635"
     CDS             21623..23464
                     /locus_tag="PC545_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2456406.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLERFEEITIFHTNDIHGRY
                     AEGDDHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMDRGKSAIELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGKKVFDSNDIKEIDGIKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVNELKGKGANVIVAICHIGTNSLSKPN
                     SIDIANEVQGIDLIIDGHSHTKFENGKQVGDTLIVSAGQYLENIGEIKLTLDTNDVEN
                     IKIENKSVRLIGKEEALKYKLDSAVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITRRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKLYPEKSSAFLQVGGISYYFNPKQKEGEKITNIQKEGKPLDLN
                     KKYVVATNDYIASGGDEFPCFSKEPVLKEFGNLESAVIEYIEYKKEISKNVDGRIGIK
                     SKDELISKNHESDLILSENEKNINDNEENLSNNIKSSDSDKNKSLDKTSARESSNLGI
                     SKREAKEKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKDKKSK"
     gene            23769..24476
                     /locus_tag="PC545_001636"
     CDS             23769..24476
                     /locus_tag="PC545_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456407.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFGIEEAKNIFAKE
                     EICLIISDIGLPDGSGFDFCEEIRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIIRTDDKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSEPKYIK
                     TVRGVGYMWGERSIKKC"
     gene            24470..25780
                     /locus_tag="PC545_001637"
     CDS             24470..25780
                     /locus_tag="PC545_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2456408.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSVLSVLIVTLVFILFEYSR
                     LHKLYEHQIKNTQNIAGILIEKYPNDEIDIVNSIYNSDYSKVSTGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKRLSKIYFIVDKMSQE
                     NYLKDTTESEETGYLVEYNEYFKEGIFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILNAVKSVTPKAKSKSITLNLNEFNSRIIPHDKKWTEESI
                     FNVLDNAVKYTQVNGEINISVEETANFFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            25897..26580
                     /locus_tag="PC545_001638"
     CDS             25897..26580
                     /locus_tag="PC545_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456409.1"
                     /translation="MKILYTENLSKHYGKGESLVRALDNVDLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDESFIKELLKSLGLENKHDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGR
                     VSKVGDKND"
     gene            26573..28960
                     /locus_tag="PC545_001639"
     CDS             26573..28960
                     /locus_tag="PC545_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2456410.1"
                     /translation="MINTTKIAKSNLKQNKSKSILIIITIILSTLMLSSIGIYIVDAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFIKGKLPSKENEIVLDSGALKALGYKNKDLGEKIKISYDDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRDMSQEDFNIYVKFNDKSNLSI
                     EQAKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGIAIPIGIILGYVLANIIIK
                     SFMDIDVKSSRLPVILSVVVISFISVVLSLLKPMKVASKVSIVDAVRYTGNKISNKSK
                     RKGYKNINLKRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKDNLNILQMDNPLSKDFFNQIKNMEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLNKNIIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTEEG
                     KYQEIKDSIKKIAKNNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAIYYTSGMMIGSILFGGILGYIAV
                     IFLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            29240..30382
                     /locus_tag="PC545_001640"
     CDS             29240..30382
                     /locus_tag="PC545_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2456411.1"
                     /translation="MNKKINYAPGPTETRENVRLARAEKTTNPDIDIDFVEFYKKTCE
                     KFGNIIGTKNDVYILSGEGILGLEAACASLTEKGDRVLVIDNGIYGEGFKDFVTMYGG
                     EYVLFSSEYTKSIDIDELKAFLDEDSNFKYATVVHCDTPTGVLNDVSKICPLLKKYGI
                     LTVVDSVAGMVGERLSVDESKIDIILGGSQKAISAPAGLTIVGISQDAKNCIKNRKTD
                     VIGFYCNLNIWEGYYEKKYFPYTMPISDIMGLDKALDNILEEGIENVLNRHEKIAYSV
                     RKSIEEYGLELFLEEGYSNTVTAIKIPESIGALELTDYMLKNYNTLVATSLNQYMDTI
                     LRIGHMGENANLNKIEHVLNVLDKSLSALGFKGNGNLLNLFNKYYF"
     gene            30401..30925
                     /locus_tag="PC545_001641"
     CDS             30401..30925
                     /locus_tag="PC545_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2456412.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGGKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIA
                     WANGNNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFIVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            31025..31843
                     /locus_tag="PC545_001642"
     CDS             31025..31843
                     /locus_tag="PC545_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2456413.1"
                     /translation="MLSVTRYSIKNVRLKPWVKFIWYFETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKIENQDYTASNMHFNGIRDKHGFIIQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSQLFAKKLEESLNPTQSVEKIVLYLEEVLLSILEEDLISNKYV
                     KLLNSFIYVNRYSNIKAFCDDTNINIKTLERICLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            31921..32232
                     /locus_tag="PC545_001643"
     CDS             31921..32232
                     /locus_tag="PC545_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDF2456414.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKALKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(32359..34362)
                     /locus_tag="PC545_001644"
     CDS             complement(32359..34362)
                     /locus_tag="PC545_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HDF2456415.1"
                     /translation="MKSQKRFKILDIDVYLQPFESDIKKRMKHYENMKSKTLKNYKDF
                     SSFANAHLYYGFHQTNDGFFYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTANGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTPFIWTDN
                     DFDLKNITYPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSMNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLAMGVPDFWIKTISNLSDEDWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNHVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYSKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDM
                     VEFGGNTKKENLRDYDVYFSSTPPSSKMPIASRTAIVLLDSSI"
     gene            complement(34515..35153)
                     /gene="recX"
                     /locus_tag="PC545_001645"
     CDS             complement(34515..35153)
                     /gene="recX"
                     /locus_tag="PC545_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDF2456416.1"
                     /translation="MGIITKIEQQKRNDDRVNIYVDDKFFIAIFKELVYTFNLKKGNE
                     INEEELKPILDDEMYIKAKNKALNILSHADQSEKKLKEKLSSEFDENIIERVIDFLKS
                     YNLVNDSVLAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTINEAVSELDKDTEFENA
                     MYLAKKRYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
BASE COUNT        13675 a         3819 c         5987 g        11787 t
ORIGIN      
        1 cttacggttc ttatatatgt tatttaaaaa taaatataaa aaaccttcaa aatacttgtt
       61 ttaagtaatt gcatatttta atattatgat aagtgatgaa aaatggaagt tactcatggt
      121 agaggatatg catatagtat gtcaaatata gacatccatt attatgggga gacatctttg
      181 gaatcctagt tatttcgttg ctactgtatt agaaaataca gaggagcaga taggaaaata
      241 tattaataat cagaaaaaac aagtaaagtg aggtgatagt agtggaaaaa gcttataagt
      301 ttagaatgta tccaaataaa aaacagcaag agttaattaa caaaactttt ggatgttgta
      361 gatttgtata taataagtat cttgctaaaa gaatagaagt ttataaaaac gataaagaaa
      421 cttttactta taaacagtgt agttctgatt tgaccaattt aaaaaaagaa ttaaagtggc
      481 ttaaagaacc tgacaaattt tcactacaaa acgtcttaaa agatttagat aatgcatata
      541 aaaaattctt taaagaaaaa gcaggatttc ctaaatttaa atcaaagaaa attaatagat
      601 tttcatataa aactaacttt acaaatggaa acattatgta ttgtggtcaa catataaaac
      661 tacctaaact tggtatggtt aagataaggg ataaacaagt acctcaaggt agaatactta
      721 atgctgctat atctaaagaa ccaagtggta gatattatgt atcattatgc tgtactgatg
      781 tagatattga agcatttgaa aatactaata atcagatagg tttagattta ggaattaaag
      841 aattttgtat ctcaagttgt ggagaattta tagaaaatcc taagtatctt aagaagtcat
      901 taaacaagct tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata
      961 gaaataaggc aagattaaaa gttgcaagac ttcaagaaca tatagctaat caaagaaatg
     1021 attttctaca aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag
     1081 atttacaagt aaagaatatg attagaaata gtaaactatc tcgcttaatt tcagatgtat
     1141 catggtcaga atttattcgt caattagaat acaaggctaa ttggtatggt agacaaattg
     1201 taaaggtagg taaattcttt gctagttcac aaatatgtaa taaatgtggg tataaaaacg
     1261 aggaagttaa ggacttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg
     1321 atagagatat aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc
     1381 aaaataaata acatatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt
     1441 aagacatatt ttagtatgca aaattctatg aagcaggaac cctgtgactt tagtcatggg
     1501 aggttcagtt tatttcaaat aatattttac gtaatccatc tatgttaatt ggattaatag
     1561 cagcatttgg tcttgtacta cagaagaaga aagcatctga tgttataaag gggtcactac
     1621 ttgcagcttt cggaattata attatggaga ctggaacagg tatgttagtt agttcaatag
     1681 caccaattaa tggagctttc caaagtataa gtggtggtgc agtaacaaaa ggtttaagtg
     1741 atgttacttt tactgcaagt tatggtggaa ttataggttt agcaatgttt gcgggtttag
     1801 ctattcacct aatgatagca agatttactc ctattaagac aatcttctta acaggtcaca
     1861 tgctttattg gttcccattc atatttgtag cttcaggagt tgaagctgga ttaaatggtg
     1921 gagtattaat aacttttgca gcagtattat cagcactcta ttggtcaata atgccttggt
     1981 tattaagaaa atatgtgttt gatgttatag gagatgattc ttttacatta ggtcatccat
     2041 ctggttgttt agcacttata tcaggatttg ttgctaaatt agtaggaaat aaagaaaaat
     2101 ctacagaaga tataaacata cctcaaagtt tatcattttt tagagaaata tctataagtg
     2161 gtggaatggc agtgttttta atgtttatag ttgttgggat agtaatacca ggtgcgttta
     2221 aagatagtaa tgagtttatt ggaacgtatg caataaagca aggtctttct tttggagcag
     2281 gtcttataat aatgcttcat ggggtaagaa tgctaataaa tcaaataatg ccagcatttc
     2341 aaggtatatc tgaaaaagtt gtgccaaatg cactacctgc ttttgactgt ccaatattat
     2401 ttaattataa accaaatgct gtattaatag gattcttagt tgctatgcta acatctacag
     2461 ctatgatttt aatatataac tactttaatg tatttaattt gctacttata ccacttgtta
     2521 taacttcatt ctttgagtgt ggagcagcag cagtaatagg agaatcacaa ggtggtatac
     2581 gtggagctat aataggtaca gctatagctg caatatttat ggtagtttta gtaggaatat
     2641 ctgctgtaat ttactcagga actattcaaa attggatact aatatttgga ggtaatgacc
     2701 tttcattatt tggtataatc agtaggttta taggttcact atttggagga atattataga
     2761 tatgaaattt aaatacatag atgagataag aagattaatt gatataatag aaaacgaaga
     2821 aaaagtcaat atggaaaaag cagtacaaat ctttgttgaa gcaatagaaa gtaaaaatgc
     2881 tatattttca tttggagctt cacatgcagg aatattaaca gaagaattat tctatagagc
     2941 aggtggctgt gttcttataa atccagtatt tgcaaaatct ctaatgttag atacagcgcc
     3001 tataactcac acaagtaaga tggaaagatt agaaggatat ggtacagctc tagctgataa
     3061 aactccaatt aaaaaaggag atgtagttat agttcattca gtatctggta gaaatccagt
     3121 aagtatagag tttgctatga aagctaagga aaataaggca atagtaatat gtataactaa
     3181 tttaagttac tcaaaaagtg tatcttcaag acattcttca ggaaaaaaac tttatgaagt
     3241 atgcgattta gtgatagata atcatggtca aaagggagat gcctgtgtag agatagatgg
     3301 attaaaccaa aaagttagtc caacttctac tgtaatagca actactgtga tgaactctat
     3361 tatagctcaa gctactcagg aattagttaa taaagggcta aagaatcctc cgattttcta
     3421 tagtgccaat attgatggtg gagatgagtt aaataagaag atttttgatg aatataaaag
     3481 tgtaattcat tatgaatatt aaggttaaaa aaatatgtta atgaactttg aagtttatca
     3541 acaaagaata ttaataatat ataataaatg ctatcattta aaagctgtag gtaaaaatct
     3601 acagcttttt attattgtag attaaaaaga tgcgtaatta aattttaaat tggagaaaat
     3661 aaattggaag gtatcaatta taaatattag aaacgttgtc atagaaaaaa actatattaa
     3721 aaaaaagtac atagaaaaaa ctgattagac aataaataca tgctttgata atattttaaa
     3781 tggaatgaaa atgtgtagaa ttataatgaa aaaagaaaat aaaaatgaaa aaagaaaata
     3841 aaatggaggg aataaaagtg ggtatgttaa aaaaattatg ttcactagct atggtagcaa
     3901 ttatgactac aacacttata acagggtgtt caagtggaaa tggaaaagat tcaaaaaagg
     3961 atggcgaaaa agaaaaacta gttctttgga tgccaccagt agaaaataat atgaaggaag
     4021 tttgggaccc tattttagat aaatttgagg aaaaaaataa ctgtgaagta gatttacaga
     4081 taattccttg ggataattat tctgaaaaat ttgcaacagc tataagtgct ggcgaaggtc
     4141 cagatgtagg atatatgtat gcagaaatgt atccacagtt tatagaaagt ggagcagtag
     4201 aagatttgac taactatgca actaaagagg ataaagagca atctatttat ataaaaacat
     4261 ctgaaatgat gggtggaatg tatggtatgc cattccaagc agcaaatcca ggggtattat
     4321 actataacaa ggatatttta gagaaactag gagaaaaacc tcctaaaaca tgggaagatt
     4381 ttaaaagaat ttgtaaaaaa gcaacgcaag atactgatgg tgatggaaag atagaccaat
     4441 ggggattagc tcagggatgg ggagctaaaa cttttggtaa cttaaactgg aactggtatc
     4501 cttatttatg gcaagctggt ggagatatat ttaatgatga tttaaaatcg gttaaattta
     4561 atgataagag tggtttagaa gcagctaatt tcttaaaaga attacaagca tatgtgcctg
     4621 aagattcttt atctaaagat agtaatgaaa tgatagaaag cgtatttgga ccagggaaag
     4681 cagcatttac aataatgtta tcttctgctg caacaagtgt atttgatgaa tcattcccgg
     4741 atttaaattg gggatttgta actggactag aagataagaa agcagcaact tttggagcag
     4801 tagaccattt atctttaatg tcatctgcta aaaataaaga acttgcatac aagttaatac
     4861 aacatatgtt aagtgttgaa tcaatgacag aatttcataa agcaatacca agagcaccaa
     4921 taacaaaagg tgaaccatat caaggtgatg aaagattcaa agaaatggtg gaaaatgaca
     4981 aaaatgtata tagaccatta gtagtaggac ctcatggagt tgaaatatat gaatatttat
     5041 ggaaagaact tcaaactatg atttcaggag ataaaactcc aaaacaagcg ttagatgatg
     5101 ctgcaaaata ttcaaatgat ttattagcac aataaaagtt taaagctgta atatgttgta
     5161 aaggacttta cagattaaaa ttagcattgt atttataaat acaatgctaa tatttatatt
     5221 ggagaggata aaatggaaaa cttagttaga gagtttgaag aaaattcaaa aagaaaaaaa
     5281 acaaaattaa atattaaacc atatatgtat atattgccat tgggaataat tttagtggcg
     5341 ttttatgtga taccaataat aatgtcaata tactttagtt ttacaaagta taatattatt
     5401 agtccagcta cttttatagg tttagaaaat tataaaaaat tatttacaga tgaaacatta
     5461 aaagtatcta ttataaatac tattaaattt actgttgttg tagtaccttg ccaaactata
     5521 ttatcattga ttttggctgt ttggattaca ggaaaaggaa atagtaaaat agcttcattt
     5581 gctaaaggtg ctatatttat accagtatta tcatctatgg ttttgatagg tatggtatgg
     5641 agggctttat tgaatggaga aggttctatt atatatcagg tattaggtgc ctttggaata
     5701 gaatcatcta aacttttagg agatagtaag acagcattac ctacacttat gtttatatct
     5761 atgtggaaaa atatagggta ttttatggta atttatattt cagctataat gaatttacca
     5821 aaacactgct atgaagtagc gaaggttgat ggggctacaa aacttcaaga atttataaaa
     5881 ataactgtac ctttattgaa accaactaca ataatggttg tatttttagg gtcgatatgg
     5941 tcattacaag tatttgactt aatttatact gtgacaggtg gaggtccagg tatatctact
     6001 atgagtatag ttatgcatgc atttaactta aactttaaaa actttaattc tggatatgca
     6061 atgactgtag caaacgtatt atttttgtta atagcagtag tttcgatttt acaaaataag
     6121 cttgtaagac gagacaattc agatttttag aaaggtaggt ataatatgaa aacagcaaga
     6181 agattattaa gtgatggttt gcttttattt atagcgtgtg caagtttggt accttttata
     6241 tacatgttga taatatcatt gaagataaca tataactcat atagtttgga tatatctttt
     6301 tcaacagtaa cccttcaaaa ctatatagat atttttacta aaaaaggatt tgcacaatat
     6361 ttttttaata cagcaatagt atctttttca ggtgtattat taaatttagt atttagtaca
     6421 ttagcaggat attcttttgc gaagatggat tttaaaggta gtgataagtt atttttattt
     6481 atgataatga ctttaattat accttcccaa gttacaatga taccactata tataataatg
     6541 aaacacttag gatggataaa tagttatctt gcacttatta tgccaatacc aactgcattt
     6601 ggcgtattta taatgagaca agctatttta ggagtaccaa aagagttatt agaatcagca
     6661 aagatagatg gttgttcgga ttttagaatt ttaatacaaa tagtgttgcc tttaattaaa
     6721 ccagcattaa ttacactagc aatatttact tttatgggag catggaacga gtttatgtgg
     6781 cctcttatag ctacaactaa agatacaatg agaacattaa cagttggatt atctactctt
     6841 aagacattcc agataactaa ttatggacag atgatggcag gggctacaat aacattttta
     6901 cctccattta ttttctattt gattttacag agtaaatttg ttgaaggtgt gtctttgtct
     6961 ggtataaaag gttaaatagt ctaatttaat aatatataaa gaaacttata taaatatgga
     7021 ggaatttaaa ctatggaaat ttcatatgta ctgaaagatt gcacattcaa acacaataaa
     7081 tactcagttg tagaagctga aacttggaaa gataaggatt tggatattgt agtagctaaa
     7141 ggtgaaaaat ttgcatttca aattatgtta aaagcaacag aagaatttaa ttgtacaatt
     7201 gataaaagta gtgctatatc atggaaaggc ctgggaaata gagtaaggtt agcattaaat
     7261 atgcctaata gtttagaaaa taattttagt ataaatatat taggatatgt tcaagatgat
     7321 acaaaggcat ttgtgaatga tgctatttta agggataaag atgtgttagt agagcaatat
     7381 ttgcctcaaa cattttggat tgaaggtcaa gtaccagaag attttgaaga aaataacctt
     7441 gatataggta ttgatatatt taaaagcttt ggatatgaag acgaagaaaa agtttgtaca
     7501 attgatgttc ctgtaaaagt aagaaatgtt gtgctaaagc ctttagatga aagcaagttc
     7561 tttttagatt tatggcaaca tccaagctgt ttagctagaa tgtataaagt tgagttatgg
     7621 tctgatatac attttgaaat agtcgataac tatttaaaag aattagcatc tttaggagaa
     7681 aaagttgcaa cagttatagt ttcagattat ccatgggcag ggcaaagttg ttataaagtt
     7741 tataaaaatc catcaaactt atatgaatat aacatggtaa gtgtatcaaa aggattggat
     7801 ggaaagatta aatgtaactt tgagagtatg gataggtata taagcatagc tgataagtat
     7861 aaaatggcta aggagataga tttatttggt ctattaggta attggtgtgc tggtgagttt
     7921 ggaaatcctg tagaaggtta taaggaccct ataagagtaa gatattttga tgaaaaggat
     7981 aaagtattta aatttataaa caatacaaat gatttaaaag aatatatagg attagtatta
     8041 aatcatttaa tagagtgtgg attatgggac agagtaagga ttatagctga tgaaccaaat
     8101 aatccagaag tagtcaaaga gtgtatagaa tttataaatt ctactgtaag cactcatcaa
     8161 gttaaatata aatcagcaac tcacgaccaa aactttttag acagagctaa agatgaaatt
     8221 gatgatatgt caataaattt aaaacttact atacaaaatt ataaagatat agaaagtctt
     8281 aagaaaaaaa tcaatgacaa gggtgggata ttgacttggt ttgtatgctg ttttccagag
     8341 aaaccaaata gttttttaag ttctcctttt gtagaaaata gaataatagg atggtatact
     8401 tattactttg gtcttgatgg atttttaaga tgggactata atttatggac agaagaccct
     8461 tggaaagatt caagttacaa attccctatt tggaaagctg gagatatgtt ctttgtttat
     8521 cctggaaaag atttaaagcc agttagaagt gtcagaatgg agaatttaag atttggaata
     8581 caggactttg agttatttac aatgttagaa aaagaaaagg gaagagagta tatagaagtt
     8641 gagcttatgc aagaattatt aaataaaaaa gaaaatgctg aaattaaagg ttttggtgac
     8701 atagaactag gatattcact tgataattgg ggatatgata aagttaaaaa atatgtatta
     8761 gagttgttag acagagtata aaaaatataa gagtgtctca aaatgaactt tttagttcat
     8821 gaggcactct ttttgtatga aatcaatata gtttcattgt ttcaacaatg aaaaatagga
     8881 ttatttctat tctaagacag ccctttagtt ttttagttgt attcaatgtt ttaaaactaa
     8941 ctataaacgc tataaagttt ataaatgaca ataattatat ataacatgat gtactttaat
     9001 ttgctataat agggattgaa taatattata tggaagataa cattgtttgg ggggattaat
     9061 ataaaaagaa taggtattat tataatgttt tttttaagta tatcttttat tggattttat
     9121 cttgtaataa gagataacag tgatacagtt aagatgcaac gagggataaa tataggtaat
     9181 gcactagaat cacctaagga ttttccgtgg gatgtaaaaa tgtcaaataa gttttttgat
     9241 gatataaaag atgcaggttt tgacacagta cgtataccag taaggttttc tgattatacg
     9301 tcagattctg ataattttaa aattgatgaa gaatttttta aaaagattga taaatatgta
     9361 gattatgcat tggataaaga cttaatagtt gtgttggatt tacaccattt tgaagaaatt
     9421 atgaaagaac caagagttca taaagaaaag tttttaaaaa tatggcaaca gatagctaat
     9481 aggtatcaaa aatatgataa aaaattggta tttgaattat taaatgaacc taaagaaaat
     9541 ttatattcac agttactaaa cgaatacatt gaagaagcaa taaagataat tagaaaaact
     9601 aaccctaaaa gaactattat agtaggacca tataatttct atcaaattga ttatttgaat
     9661 gaattaaata ttcctaaaga ttctaatata gtagtttcct ttcactatta cgagccaaat
     9721 gattttgcat ttcaaggaaa tatatatcat aagggatttg agcacttaag caatattact
     9781 tgggaaggaa caaacgaaca aatggattat ttaaaaaaaa ggtttgatac tgtggaaaat
     9841 tgggcaaata aaaataaggt aaaaatcttc ttgggtgaat ttggaataac aaaagaggca
     9901 ccagaagctt caagaagagc atggataaaa gctgtaagag aagaagctga aaaacgaaac
     9961 ttttcatggg cttattggga gcttgcatct ggatttggta tatacaatca aattgaaggg
    10021 acttgggata gagatatatt aagtgcatta atagaaaaga ggtgatttta tgaagaaaaa
    10081 ttcaaaaaag atacttataa ctttaactat tattactaat gtcatatata tattgtggag
    10141 aattttttac acagtaccta aagaagaagg gaagtttgcc ttaatatgtg ccataatact
    10201 tctttttgta gagataatgg gcatgatgga aatgtttgtg cattattatg gtatgtcaaa
    10261 tattgagtac cctgaaaaac caatcataag tgaagaactt tatccacatg tagatgtatt
    10321 cattgcaaca tataatgaat ctgttgattt ggtgagaaaa actgtaaatg ggtgtattca
    10381 tatgcagtat ccagataaga aaaaggtaca tatatacata tgtgatgatg gaaatcgtga
    10441 agaaatgtgt attctagcag aaaaaatggg tgtaaattac ataactagaa ctgaaagaga
    10501 aggagcaaaa gctggaaatt taaataatgc aatgcaacat acaaattccc ctttgattgc
    10561 tacatttgat gcagatatga taccaatgca tgacttttta attgctacag taccatactt
    10621 tttgaaaaat gagcaagcta aaaaagatgg agaaaaagag gagtatgaaa aggttggttt
    10681 tgtgcaaact ccacagagtt tttataatcc agacttgttt caatttaatc tgcactctga
    10741 aggaaggatt ccaaatgagc aagattattt ttacagagac attcaattgg ctagaaatag
    10801 gacaaattct gtaatttatg gtggtagtaa tacagttatt tcaagggaag cacttgaaga
    10861 agttgatgga ttttatactt attctattac agaagatttt gcaacaggta tcttaattca
    10921 aagtaagggg tatagatgtt atgctattcc agaagtacat gcatcaggtt tatctcctac
    10981 agatttaaaa agtcttatta agcaacgtga aagatgggca cgtggatgta tacaaactgg
    11041 tagacgtcta aatattttgt ttaggagagg tttaggtttt tggcagaaaa taagttatat
    11101 ttcttcaatt acttattggt atgcaagtat aaaacgcttt gtatatataa tggcacctat
    11161 tttattttct gtgtttaatg tgattgtagt aaaatgtacg ttacttcaag tattggtatt
    11221 ttggttgcca atgtatattt taagtagttt atctcttaaa atattttctc aaaatataag
    11281 aaatactaga tggaccaata tatatgaaac tataatgttt cagtctttaa tgccagcagt
    11341 aatacttgaa acgtttgcaa tatctaaaaa taaattttca gtaacaaata agagtaaatt
    11401 agaagaaaat agaatgtata agtttttaca gggaatacct tattttattt acatggtctt
    11461 atctatcata ggaattttaa aaatgtttgt agcaatattt aaaatgagtt caatgacata
    11521 ttcagttgta ttattttggt tgattggaaa cctttttaac cttgttatgg ctacattatt
    11581 tatatcaggg agacagcagt taagaaagtc tgagcgttat attgctgaaa ttgattttaa
    11641 gttaaagcaa aattcttatg ttctttcttc aaaaacaatt gatatatcag aaaatgggtt
    11701 tgcatttttg ctagaaaatc cagaatatat ttccccagaa gaagagtttg aggtagagtt
    11761 tagagaaaaa tctggaaatg aaatgtatat agctaatatg aaagcaaaga ttgttaatgt
    11821 tgtggaagta aattcaaagt ggaaatatgc agcttatatt acacatattg aagatagtga
    11881 aatagataat tggatgtgta ttgttcacga tagaatacct actttaccaa tgacaatatc
    11941 taatcaacta ggtttctttg atgatttgca aattaatgta aaaaaacgta ttgaaaaaac
    12001 tagaacatta tcacgaagaa gtccaagaat caatatgaat tttcagatgg atataaaaaa
    12061 tacaggtaaa ttaagaatag tgaattttaa ttatcaatat gtattattaa attttgagaa
    12121 taaaagtatt tatcctaaag aaatagcatt ggaaattaat gaaaatattg tgttagaatg
    12181 tgatttgtgt gagggtaaaa ttgatgaaag aggtatctta tataaagtaa ataacattga
    12241 cagcataatg caaaatttct ttttaagaga tgaaatgatg gattggatat tacaaaataa
    12301 gaaaattctt gattcaaagc ctagtgaaaa gaaggaaaaa agtatagatg agtttgaacc
    12361 catggaatat atttaggaga gatgaaatga agactttaaa agtgtttttt tgttctacaa
    12421 aatatcttat tttggtgttt atttttttta taaatactac ttttataagt tcagcaacaa
    12481 gtgataaaat tttagattta tcctttaaaa agatagagac tgacttaagt tctaagatta
    12541 catatgagga tacaggtgta aaaatagaaa cggatagttc taaaagcgat aaggaacgtt
    12601 atttatatat ttaccaaaat ataaaagaaa attggagcat gtataataat ttttatattg
    12661 aaattcaaaa taaaaataaa agttctcaga gaataaattt aagcatacaa agtaaaaata
    12721 tgcttgaatt tagattaaaa gaaggtagtg aagtattttt agaaggtaaa aatataatat
    12781 attcagataa aataaaagaa ggatgcatag aagtaccagg tgaatttgaa ggcaaaattt
    12841 atgtgaactt taatagcctt ataaatgagg aaagtaatgt tgtgctggac tcaaatatgc
    12901 tttcaaacat tgttagttgg ggaattacgt ttattccatc tgatgaagaa cataatattg
    12961 ttattataaa aaagatatct ttattgtctg aagagaaatt aagattttta aataatatta
    13021 aaataattgg cgatgaagaa gtacaaatac cagtattggg tcaaagtata agtcagtatg
    13081 aggtattagg tcttaaaagt gattctaaaa ttaagtactc tcttatggga aaacaagata
    13141 atgtgagtat atctcaaaac ggtaaattaa cattaagtaa taaagccaaa cctggacaaa
    13201 ttattttaca agttaatgtt gatgataagt ttaaaattgg aaagaaaata acattaactg
    13261 aatcttggag tattaataaa aaagataagg atggtgttcc atatacatta gtatctcctg
    13321 agcaatcacc aacagtacaa gatatgaaaa aaataaattt tatgaataat atcataacgt
    13381 ttgttcgaat tttatttgta tctctagtaa taatatgctt tggaatatat ttatattgga
    13441 aaaaatgttc aaaaactaag taaagagagg aggttagatt gtgttatttt caagtttaat
    13501 ttttttattt tattttttac ctataacttt ggttttatat tatgtattta ggtttaaccg
    13561 aacgatacag aacatgatat tgcttgctgt tagtttattt ttttatgctt ggggagagcc
    13621 aaaatttgtt gttatcatga tagtttccat aataatgaac tatatatttg gtttactggt
    13681 agatagatat agggaaagta aaataaaagt aaaagttttt ttagttttga tgtgtgcata
    13741 caatataggt gttttattta tatttaaata tctagctttt gcgctaagaa atattagtga
    13801 acttattagt actgaattga ctatacctaa tattatttta cctataggaa tttcattttt
    13861 tacatttcaa ggaatgtctt atgttataga tgtatatcgt agacatggag aagtccaaaa
    13921 gaatcctttt tatgtgggac tgtatatagc atttttccca caattaatag caggtcctat
    13981 agttagatat gagagtgtgg cagaacaaat tctcaataga aaagaaactt ggaataaatt
    14041 ttctattggt acttgcaggt tcataacagg tctaggtaaa aaagtattaa tttcaaataa
    14101 catggctata gtagcagact atatttacac tatgaatagt caaggagaaa tagctgcatc
    14161 acttgcatgg cttggttcaa tagcctatac tctgcaaata ttctttgatt tttctgctta
    14221 ttcagatatg gctataggct taggtcttat gtttggtttt aaatttgaag agaactttaa
    14281 ttatccatat atatcaaaat caattagtga gttttggaga agatggcaca tttctttggg
    14341 aatgtggttt aagagctata tatactttcc gcttggaggt tctagagtag caaataaaga
    14401 tattatgatt agaaatatgt ttatagtgtg gttattcact ggaatatggc atggagcaga
    14461 atggacattt gtaatttggg ggattttaaa ctttgttttc ctaattatag agcgatttat
    14521 aatgtttgaa aagattgaaa atcacaattt tataaaacat atttatgctt tattagtagt
    14581 taattttgga tgggtattgt ttaggtcacc taatttaaaa gaagcatata attattttaa
    14641 agctatgttt ggaggcaatg gagctatatg gagtgattat acatatatgt ttttaaaaga
    14701 atactgggtt ttctttatat ttgcatttat ttttagtgta cctattgcaa agaaaatcaa
    14761 taagtttgtt gttgaacaag ctaaatatag tatgatattt aacgtatttt atccaatctc
    14821 agctatttta ttgtttttta tttctgtgac atatttagta actggaagct ataatccatt
    14881 tatttatttt aatttttagt tgggaggaaa agtatgaata gattttttaa agtaagatta
    14941 ataactgcaa ttatgttctt aattatttta ttcgctttct ctatctttaa tgtttgttat
    15001 tctggaaaag atacgataca ggaaataaaa aattcaatag ctgaaaaaag tagtttaaaa
    15061 gatacaatat catctattga taatacagtg aatgacaaag taatatacaa aaatacgttc
    15121 attgaaactt atggctattt acaaaaatta atgggtaaaa atgagttttc aaaatttagt
    15181 gttgtaaaag atacttctgg aaagatgcat tatacatatt ttgctaaagc tccaaatcca
    15241 gttggtgtga tagcagatag ggtaagaaaa ttttctgatg aaatggaaaa gcaaaacact
    15301 aaattggttt acctaatgac tccagataag tatataaaag gagtgacaaa atttccaaaa
    15361 ggaatacctt attcttatag taatgagaca gcagataatt ttttaaatca actaaaaaag
    15421 tataatgtgg actatattga ttttagagaa aatattttaa agagtggtat tccaaaagat
    15481 gatttatttt ttaaaacaga ccatcactgg aaagtagaaa cttctttttg ggcatttgga
    15541 gaattagttg agcaacttaa taaaaaatac aatatgaatt tagatgaaaa tcattattat
    15601 agagataagg acaattataa ttcaattgta tatcctaagt catttcttgg ttctatggga
    15661 agaaagactg gtatcttata tggtggtata gatgatttta cattaattta tcctaaattt
    15721 aagactaatt atacttatta tacagattct aaatctcaaa aatttgaatt aaaaggaagg
    15781 tttgaagaga gtttaatatt atcatatcct tttaatgcag accttgattt aatggatggt
    15841 caatcagata agtattttac atatttattg gggaacagac cattagttaa aataaaaaat
    15901 atagaaaatc cagatggatt aaaagtgctg tttgttaaag attctctaat tgtaccaaca
    15961 gcagcatttt tttcctctgt ttgttcatct atagatatga tagaccctag atattatgat
    16021 ggagatatac tagaatatgc aaaatcccat gactatgact ttgtttttat atctgtatat
    16081 ccccaaaatc ttacaaaaga attttttccg ttttgtgagt aaatttctaa aaaagttttc
    16141 tgataaaata aatggctaac tggtaaaaag ttagccattt attttgagtg ttattctaag
    16201 aaatattttt tttactaaat acttctttat ctatttttcc aagtgatttt cctacagtaa
    16261 ttgatgatac catagcacca ttaacattta tcatagtacg tcccatatct agtatagggt
    16321 ctattgcaag tataccacca actaatggaa aataagaacc aagaccaact ccagagataa
    16381 caactgaaac ggccattgta gcagttcctg gaagaccagc tattccaaac gaactaattg
    16441 ttataacaat taacaacata acataaaagc tcatatccat gtttactcca gccatattgg
    16501 caagtgttac agccattaaa gcaggatata tacctgcaca accattcata cccatatttg
    16561 cacctaaact tgctgtaaag cttgatacac cctcatcaac tccgtgattt tgtactagac
    16621 tttctatcgt tacaggcaat gttcctaaac tagaacgtga agtaaatgcc agaagtaaag
    16681 cttcagttga attttttata taagtaatgg gatttagacc atttaaagtt ataaaaatta
    16741 aatgtataat aaataatatt gcaactgata tataaagagc taatataaac tgtaaaacag
    16801 atactattga actaatacct tgtgaagtta ttgtatttgc aagcagagca accacagcat
    16861 aaggcataaa tttaataaca gtcatagaca cacttacaat tattttgtaa gatgcttcta
    16921 taaaatctat aaatggtttt attgtctcaa aatgtttttt acttaatctc tttattgagg
    16981 tagctataaa tgtagcaaat attattgtag ctacaatatt tccatttgcc atagccatta
    17041 caggattaga tggtagtaat cctcttaaag tatccacaag agagtttatt tctttaagtt
    17101 cttgattttc aactaaagta ggcatattag ctcctacacc taacttcatt atatttgcaa
    17161 cgataattcc aacaattgca gctatagcag tagtagcaag aagcatacct aaagatttaa
    17221 atgtcagagt tccaagattg ttatctttca tatttataat tacttttata atggataaga
    17281 aaactaaagg tactactaac atttttaata agtccataaa tccagaacca aataggctat
    17341 accacttact tacttcttgt agccaagtga cttgagttgt atcattagga aaaccagcaa
    17401 aagcttgtat tatgattccc aatatgagtc ctagtactgt agcatatatt gttctcaatg
    17461 aaaattttat ttttttcttt tcaaattgtc taacaataaa gaacgtacct agaagtaata
    17521 ttatgaaaaa aattgtttta aaatctgtaa tcataagaaa ttgtgataaa aatgaacttt
    17581 ccaatatttt tctcctttta tttaaaaata aatttttcta agtttaaatg atatcaatat
    17641 attaaaaaaa tgtcaatatt atatcacaat ttacttaaat agagtaaaac aagttgattt
    17701 tgaaaaatgt gattaagtac agaaaaatag ggtataataa atgtttaaag aatttgaatt
    17761 gtggtaagaa aataaaaaat agcttttata agatttgatt tcaaattaaa atatgttaaa
    17821 taaataacta aaaattatta taaaaaatgt tttcatagaa tatatatcaa atgaaaagct
    17881 taaaataagt gaagaaatta actagacatc aaaggagaga taaaatgaga gtaataataa
    17941 ttggtggtgt tgcagctggt atgagtgctg cagcaaaatt aaaaagaata aaacctgagt
    18001 atgaagttgt tgtttatgaa aaaactgaaa tagtatcttt tggtgcttgt ggattaccat
    18061 actttgttgg tggatttttt aatgatgctg atgaattatt agcaagaaca ccagagaaac
    18121 ttagagaagc tggaattgat ttaaatatat ttagagaagt cgttgaggtt gatagtgaat
    18181 ctaagaaaat aaaagtaaaa aacataaaaa cagatgaaat atatgaagat tactatgata
    18241 aattgatgat agcaactgga gcaagaagta ttatgcctcc tataaaaaac ataaaattga
    18301 agaatgtttc tacattaaaa agtttatatg atggagaata tctaaaaaaa cttttaagta
    18361 atgaagataa taaaagagtg acaataatag gagctggttt tatagggtta gaagcagttg
    18421 aggcatgtaa gaaattaggt aaagatgtag atgtaattca gttagaagat agaatattgc
    18481 cacaagtatt tgataaggaa atgacggatg tattagaaga agaaataaat agacataatg
    18541 ttaatttgca cttagatgaa atggttgtag aactttgtgg agaagataaa gtagaaaaag
    18601 ttataacaaa caaaggggaa atagatacag atgtagtaat aattgctact ggcgtgagac
    18661 ctaataccga gtttttgtca aatactaata taaaaatgct aaaaaatgga gctatagtag
    18721 ttgatgagta tggaagaact tctgtagaag atatatactc tgctggagat tgtgcaacta
    18781 taaagaatat agtaagtaat gaaaatgtat atgttccttt agctactgga gctaataaat
    18841 tgggtaggat agtaggagaa aacctagcag gaagagaagt atcttatcaa ggttcactat
    18901 cttcaagctg tataaaaatt atggatatgg aggcagcatc aactggaatt acagaaaagc
    18961 aagctaaaga tttaggcata aatgtcaaat ctaaatttat ttctgattat aatcaaactc
    19021 attattatcc aggaagaaat aaaatatatg taaagcttat ttatgatgca gatacaaaag
    19081 ttattttagg cggacaagtg gctgggttta aggatgctgt ccaaagagca aatgtattgg
    19141 cagctgctat ctttggaaaa atgaccacaa atcaacttgg aatgttagat ttatgttatg
    19201 ctccaccatt tgcaagaaca tgggatgttt taaatgtagc tggaaatgta tctaaataat
    19261 atttagatta tagctaatat agttgacaag caattaaggg aatgaattta cctaaactat
    19321 ttttatcatg gtaaaattca ttcctaaatt tttattccaa atttttattc caaattttta
    19381 gaaataagga ttccattaaa ttattcatca aaatttaaac gcaagcttaa tctatcttct
    19441 ccaaggatag tattttcaaa aacattagta gcattttcta aatacatact aggtatgtct
    19501 aaagaaggac taaaatgagt cagtaaaagt tgtttgacat ttccaagttt tgcaagatta
    19561 gcagcttctc taaaagtcat atgtttgttt ctaacagctt ttgatatatc taaatcatca
    19621 ccatacatgg cttcacatat aaataaatca ctatctttta tgaaatctgg tattgattct
    19681 ataggtcttg tatcggttgt aagacttatc ttaactccct ttctcaattc tcctaagacc
    19741 atatctggag tgtattgttt tgaatctaaa actatatttt gaccttcttg gagtttgttc
    19801 cataatattt taggaacttc gtttttagtt gccttatcta tatcaaattt agggtttctt
    19861 ctaaaattaa aactgtatcc taaacactct atagagtgtt ctaaactgat tgttgaaatt
    19921 tctaaatttc ttaaaacttt attatctaaa gaaaaattgc cttgagggtt ttctataatt
    19981 tttaaagtgt atggtacata ttctactaaa tttctcatag aacgtataca atcgactatt
    20041 ccaactggac caactatagt taaatcactt gtacgaccac tatttccaat tgttgataaa
    20101 agtcctaaaa gtccaaatat atggtcacca tgtaaatggg ttatacaaat caagtctata
    20161 tctttaaagc cacatttttt tagcttcata gatacctgag taccttcacc acagtcaatt
    20221 agtatttttc tacctttata gtttataaat acagaggata gaaatctgtt tggcataggt
    20281 acatttccac cacatccaag taaagttaaa tctatcattt tttccccttt ctaaaattaa
    20341 cacaaatctg ttgtttgttt ttattatgat atcctattat aattatttta acattaaata
    20401 taacaataaa tgtgtatttg agtcaagaaa tattgtcaaa taaagttttt atatagaaat
    20461 gtataaaaat ttggaaaaca aaaagtgaaa attatattta ggagcattta aaggaggaaa
    20521 atttaatgaa agtattaaag aaaattacta ttttgtcatt aattacatta accttagttg
    20581 gttgtagtaa cggagaagat aaaaaagtgg ttgaaggttc taagaaaatt caaaattcta
    20641 aaaaagatga acagcctata atagtacata aagatgcaaa tatattgaaa gataggtatg
    20701 agccaccaaa aggatttaaa agagttgatg tagaaaaagg tagttttgaa tcatttctta
    20761 ggaatactaa gctaaaaaat tatggagaga aagtaaagta ttatgatgga agaactaagg
    20821 aatcaaaagg tatttatgat agtgtatttg atgttgatat tggcaataga gatttgcatc
    20881 aatgtgcaga tgcaattatg cttatgagag cagaatactt gtatcagaat aaactatatg
    20941 atgaaataag ttttgatttt gttgatggtt ttaaggcgaa gtattcaaaa tgggcacaag
    21001 gatatagaat ttcagtaaaa gatagtggtt caagctggta taaagcaact gaatattcaa
    21061 catcttatga gagttttaga aagtttatgg atatagtgtt tgcatattca ggaacgttat
    21121 ctcttgaaaa agaattggag cctgtaaaag ttgaggatat gaaaatagga gatgtgttta
    21181 tagttggtgg aagtccagga catgcaagta taattatgga tatggcagaa aatgaaaaga
    21241 ctggtgaaaa ggtatttatg atagcccaaa gctatatgcc tgcacaacaa acgcaacttc
    21301 ttattaatcg taaaaatcca aatttgagtc cttggtatag tttagatttt gaaggtgatt
    21361 tgacaatacc tcaatggact tttaaaagag accagttgaa aagattttaa aataataatt
    21421 ttatataata aaagaaaaat atagatgtga aaaaatgtaa ttagattaaa actaattgga
    21481 tatggacaaa attaaattga tagataaata ttaaaaatta cactaattaa tcttactata
    21541 gtttaatatt tatctaaaaa gttttatgta aaatataaat tttatattaa agctaaaact
    21601 aatttgagga ggctaattta tattgaagat tagaaggtat ttaattttgg taattataat
    21661 tctaattaca acaagtcaga gttttatttc ttatgggcta gagcgatttg aagaaataac
    21721 catttttcac actaatgata tacatggaag atatgctgaa ggtgatgacc atatacaaat
    21781 aggaaatcta gccacattga aaaaagaaac tccaaactca attttagtag atgcaggaga
    21841 ttgtttgcat ggacttccaa tagtaaatat ggatagaggg aaaagtgcaa ttgaattaat
    21901 taaatgtgct ggatatgact atattactcc tggaaatcat gactttaatt atggaaaaga
    21961 tagactgttt gagttatcta aaagtgctaa tttaggagag aatactttaa aatttttatc
    22021 atcaaatatt tttgaaaatg gaaaaaaagt ttttgatagt aatgatataa aagaaataga
    22081 tggaataaaa ataggatttt ttggactctc aacccaggaa acaaagagca aaactggatt
    22141 taaaaatgtg gaaggattag agtttaaaaa tcctatagaa agtgcaacag aacaagtaaa
    22201 tgaattaaaa ggaaagggtg ccaatgtaat agttgcaata tgtcatattg gtacaaatag
    22261 tttaagtaag ccaaatagta tagatatagc aaatgaggtt caaggaattg atttgattat
    22321 tgatggacac agccacacta agtttgaaaa tggtaaacaa gtgggtgata cattgattgt
    22381 aagtgcagga caatatttag aaaatattgg cgagataaaa ctaactttgg atactaatga
    22441 tgtagaaaat ataaaaatag aaaataaatc agttagatta attggaaaag aagaagcatt
    22501 aaagtataag cttgactccg ctgtaagtaa aaaggttgat gaaataaaag aacaacaaga
    22561 aaaaatatta gataaagtta taggggtaac tacaaatact ctagatggtt catatgaaaa
    22621 tgtaaggaca aaagaaacta atttgggtaa tttaataagt gacattttac tagataaaac
    22681 taaggctgat atttctttat ttaatggagg gaatattcga gatacgatag aaaaaggcaa
    22741 tataacaaga agagatattg tagatgtatt tccttttagc aatactatag ttaccaaaga
    22801 acttacagga gcacagatta aagatgtatt ggagcatgga gtaaaacttt atccagaaaa
    22861 aagttcagcc tttttgcaag tgggaggaat ttcatattac tttaatccta aacaaaaaga
    22921 aggcgaaaaa ataacaaata ttcaaaaaga aggaaagcca ttagatttaa ataaaaagta
    22981 tgtagttgca acgaatgatt atatagcatc aggtggagat gaatttcctt gtttttcaaa
    23041 agagccagta ctaaaggaat ttggaaattt agagagtgca gtcattgaat atatagagta
    23101 caaaaaagaa atatcaaaaa atgtagatgg aagaattggt attaaatcta aagatgagtt
    23161 aataagcaag aatcatgaga gtgatttgat attaagtgaa aatgaaaaaa atatcaatga
    23221 taatgaagag aatctatcta ataatataaa aagctctgat agtgataaaa ataaaagctt
    23281 agataaaaca agtgctagag aaagttctaa tctaggaata tctaaaagag aagctaaaga
    23341 aaaatctcct aaaacaggag atttaggttt ttcaaatagt ataattatat ttatagtttc
    23401 aagtacattg atatgtttat tgaattttaa tcaaaaagag ttaaaggata aaaagtcaaa
    23461 ataaaaatta atgagcaaaa attagtagat agtaatttaa ttttagataa tagtaaacag
    23521 caaaggaaat tagttatata aatactaatt tcctttgttt tatgttttaa catgattact
    23581 tttaaataaa attgtatttt atgaaaaata attacactaa atggcatagt attatgggga
    23641 tttttagttt ttattcaagt aaatgaaatc atattaacaa cttctgatat atttacaaat
    23701 tgataaaaaa atgattttat tagtatgata taatttagct agtatatatg atgatggagg
    23761 atatagggat gaataatata ttacttttgg aagatgacaa atctttaaat agaggaatta
    23821 gttttaaatt aaaaaaagaa ggatacaatg tttttagtgc atttggtata gaagaagcaa
    23881 aaaatatttt cgcaaaagaa gagatatgct taattatttc cgatataggt ctacctgatg
    23941 gtagtggttt tgacttttgt gaggagatta gaaagaaaag tgatgtttac ataataatgt
    24001 tgacagctct tgatgaagag gtggatattg tcacaggata tgatttaggt gcagatgatt
    24061 atatcacaaa gccttttagc ttgatggtat taatatcaaa agtgaatgcg cttatgaaaa
    24121 gagtaaatac agtaaaaaat tatactctat tagtttgtga tgatttattc ttttattata
    24181 tagaaaataa attaattata agaacagatg ataaggaaga agagataatt ttaagtaaga
    24241 cagagacaaa actattaaaa tatttgatgg aaaattctat gcagacattg actaaagagc
    24301 aactgttaga atctttatgg gatagtagtg gtaattttgt agatgataat acgattgcag
    24361 taaatataag aagacttaga caaaaggttg aaaaaaatcc atctgaacct aaatatataa
    24421 aaacagtaag aggggttgga tatatgtggg gagaaaggag tataaaaaaa tgctaaaaaa
    24481 tatgaacctc aaagatggtt taagtgtgaa agatgattat ataatgagaa gagttattgt
    24541 atattctgtt ttatctgtac taatagtaac ccttgtattt atactttttg aatatagcag
    24601 attacataaa ttgtatgaac atcaaataaa aaacactcaa aatattgctg gaatattgat
    24661 tgaaaaatat cctaatgatg aaatagatat agtaaattct atttataatt ctgattatag
    24721 taaggtgtct actggagaaa aggcatttaa gaaatttggc tatggattgg aaaataaaat
    24781 gtcaaatgat aaaaatttta gcttatactt aaaatctttt tttaaggaat cattttttat
    24841 ttttttaatg atgtttatag tagttatagg tataatatat tattttataa gatacataaa
    24901 taaaagattg agtaaaattt attttatagt agataagatg tcacaagaaa actacttgaa
    24961 ggacactact gagtcagaag aaactggtta tctagtagaa tataatgaat actttaaaga
    25021 aggcatattt agtaagataa ataactgtct atatgagctt aatagaagtt taaagataaa
    25081 gtttattaaa ttagaaaaag aaaaagaaag tgtaaaatca ttagtaacag atatttccca
    25141 tcaattaaaa actcctttag catccttaaa attatacaat acattactta tagaagaaga
    25201 attagaagaa gatgaagcca acgagttctt attaaccaat aaggcttcta taaataaatt
    25261 agaaaattta ataaattctt tagtgaacat atcaaggtta gaaattagca tgataaacat
    25321 aaaaaaagaa gataatgata taaaaaatac tattctaaat gcagtaaaaa gtgttacccc
    25381 taaggctaag agcaaaagta taacattaaa tttaaatgaa tttaacagta gaattatacc
    25441 acatgataaa aagtggactg aggagagtat ttttaatgta ttagacaatg ctgtaaagta
    25501 tacacaggta aatggagaaa taaatatatc tgtagaagaa actgctaatt tctttaaaat
    25561 tataatagag gataatggca tagggattga aaaaaatgag tttaataata tatttaaacg
    25621 tttttataga ggttctgctg atgaaattga atctgtagaa ggtagtgggg taggattgta
    25681 tttaagtaga aagattttag aagaacaagg tggaaatatt atagtttctt caaaagtagg
    25741 aatagggagc aagttttctt tgtttcttac aactatgtaa tattaaatga aaggaaaatg
    25801 taagcctcta catttattat ataagtataa acagctccag aaataaaaaa ttaataaaat
    25861 atggagtaat ataacttaga atgagaggat aattttatga aaatactata tactgaaaat
    25921 ttatcaaagc attatggaaa aggagaaagc ttagtaagag ctttggacaa tgttgactta
    25981 gaaataaatg aaggtgaatt tgttgcaata ataggaaaat caggttctgg aaaaagtaca
    26041 cttttgcata tgattggagg tcttgatata cctacatctg ggaaggtgta tatagataat
    26101 aaaaatatat ttacattaaa agaagaagaa cttgctgtat ttagaagaag aaaaataggg
    26161 tttatatttc aaagctataa tttaattcca tctcttaatg tatgggaaaa tgtagtgttg
    26221 ccaatcggtt tagatggtag agaagtagat gaatctttta taaaagaact tttaaaatca
    26281 ttaggacttg aaaataaaca cgacgtttta ccaaatacat tatcaggtgg tcaacaacaa
    26341 agggtggcaa tagcaagagc attagctaca agaccagcta taatacttgc tgatgaacca
    26401 actggaaacc tagattctaa aacgtctgat gaggttatga gtatattaaa atctatgtct
    26461 aagaagtaca gtcaaactct agttatgata actcatgatg acagtatagc ccaaatggct
    26521 gatagagtta tattcataga agatggcagg gtgtcaaagg ttggtgataa aaatgattaa
    26581 tactactaaa atagcaaaaa gtaatttaaa gcaaaataaa tcaaaaagta ttctaataat
    26641 aattactata atactttcaa ccttaatgct ttcttctatt ggaatttata ttgttgatgc
    26701 aggagcatat caaaaagaaa atactataaa gtattcaggc aattatcaag gtatattagc
    26761 aaatgtagat gaaaaacagg ctgatatatt gagtaatcat gcagatgttg aattaactgg
    26821 tgaaatgaat ggagtaggtg ttgaaaaact ggaagatgat tcaaatattt ctctagcata
    26881 catgaatgaa gatgctttga aattaaatag ttttgaattt atcaaaggaa aattgccatc
    26941 aaaagagaat gaaattgtgt tagatagtgg agctttaaag gcacttggtt ataaaaataa
    27001 agatttaggt gaaaagataa aaatatctta tgatgactat aaaaatgata aaaaaattga
    27061 aaaagagttc attataagtg gaatcttaaa aactagcgaa atatctgaag caggtaaata
    27121 ttattatgct attatatcag aatcatatat gagaaatact agagatatgt cacaagaaga
    27181 ttttaatatt tatgtgaaat ttaatgataa aagtaattta tcaatagagc aagcaaaaga
    27241 aaaattagat aaaattgcta atgatatagg actggatact attaatactg ctgtaaatga
    27301 aaattatata aatgctttaa aaccagatat ggaaacgatt atgggtggag tttttgtagg
    27361 gttagtaatt gtattatcta gtattttagt aatatacaat atattttata tttcaatagt
    27421 tacaaaggtt caagaatttg gaaaacttag agcaattggt gcaactaaga aacaaataaa
    27481 aaatatagtg tttaaagaag ggttcatatt agccggaata gctataccaa taggaataat
    27541 tttaggctat gtacttgcaa atattataat aaaatcattt atggatatag atgtaaaatc
    27601 atcacgatta ccagttatat tatcggttgt ggtaataagc tttatatctg ttgttttatc
    27661 acttttaaaa ccaatgaaag tagcatcaaa agtatccatt gttgacgcag taagatatac
    27721 aggaaataaa ataagtaata agagtaagag aaaaggatat aaaaatataa atctaaagag
    27781 attatcacat gccaatttag aaagaaataa aaaacgtact tacatgacat tggcatcact
    27841 tatacttagt ggaactatat ttataacagt atcaactgcc ttagaaagtt ttgatgctga
    27901 gaaaatggca agagaacact ttccttatga tatagaagtt agactatctg gttatgaaat
    27961 gaacagtgat aaaaatccta aagataattt gaatatttta caaatggata atcctctaag
    28021 taaagatttt tttaatcaaa taaagaacat ggaaggaatt aaaagaatag aatctgcaag
    28081 aagtgtaaaa attggtatgg aggactatga tgttgaattt aagtatgacc ttttacaaag
    28141 tataaatgaa aatgatgtaa aatcattaaa caagaatata atagatggaa aaataaatct
    28201 tgaaagactt caaactggag atgaaatagt aattactcat gttgatacag caaaagaaat
    28261 gggagtaaaa gcaggagata aaataagatt aactttatat gatggagata aaaagataaa
    28321 aaaagaattt aaagttcaag caatagctat gggggttcca agttttggta tagggaaaga
    28381 ttttatagat agaactttga aatacgattc aactagtagc ttgggaatat atacagaaga
    28441 aggaaagtat caagaaatca aagatagtat caagaagatt gctaaaaata atggattttt
    28501 agagactgat tttatagata gtagaattga gtcaaataag gctactattt catttataaa
    28561 gataatggga tacacgctaa caggcataat aggtgtgatt ggatttatga atcttgtcaa
    28621 cacaatgata actagcatag ttactagaaa aaaagaactt ggtatgcttc aagctattgg
    28681 tcttactaat aaacaattag tgaaaatgct taattcagag gcaatatatt acacttctgg
    28741 aatgatgata ggaagtattc tatttggagg tatattaggg tatattgctg ttatatttct
    28801 aaaaaagact ggattatctt atgcaacata ttctttgcca atagtaccaa tcttgttgat
    28861 gattgtgtgt attcttatag ctcaatttat aacaacttat ttgataggac gaagttttaa
    28921 taaagaatct cttattgata gagtaagata cagtgaataa aatgcaaata tttatggtta
    28981 gtttaactag caaaaaacat ataaaaaaat atagttaaag agattcataa ttaaataggt
    29041 taaagcatag tacagagcaa acaatttaaa cagatgtttg ctctgtattt tattatgtaa
    29101 tcaataatat atctaatata ctgatattat tcatgttaga aaatgtaccg gtacaattct
    29161 aaaaaagtat aaaattgtac catataaaat gaaaattttg tataataatc gtatagaaaa
    29221 taaataggag ctgatatata tgaataagaa gattaattat gcaccaggac caactgaaac
    29281 tagggaaaat gtaagattag caagagcaga aaaaacaaca aatccagata tagatattga
    29341 ttttgttgaa ttttataaaa agacatgcga gaagtttggt aatataattg gaacaaaaaa
    29401 cgatgtttac atattgagtg gtgaaggaat attaggtctt gaagcagcat gtgcatcact
    29461 tacagaaaaa ggagatagag tacttgtaat tgacaatggt atatatggag aaggttttaa
    29521 agattttgta acaatgtatg gtggggagta tgtgcttttt tcaagtgagt atactaaaag
    29581 tatagatatt gatgaattga aagcattttt agatgaggat agtaatttta aatatgcaac
    29641 agtagtgcat tgtgatactc caactggcgt attaaatgat gtaagcaaaa tatgtccatt
    29701 acttaaaaaa tatggaatat taactgtagt tgattctgta gcaggtatgg taggagaaag
    29761 attaagtgta gatgaatcta aaatagatat aatattaggt ggttcacaaa aagctatttc
    29821 tgcaccagca ggacttacta tagtcggaat aagtcaagat gcaaaaaatt gtataaaaaa
    29881 tagaaaaact gatgtaatag gtttttattg taatttaaat atatgggaag gctattatga
    29941 gaaaaaatac ttcccttata caatgccaat aagtgacata atggggcttg ataaagcact
    30001 tgataatata ttagaagaag gaatagaaaa tgtattaaat agacatgaaa agatagctta
    30061 tagtgttaga aaatctattg aggaatatgg tctagaacta tttttagaag aaggatattc
    30121 taatacagtc acagctataa aaattcctga aagtatagga gctttagaac ttactgatta
    30181 tatgcttaaa aattataata cacttgttgc aacatcacta aatcaatata tggatactat
    30241 tttaagaata ggtcatatgg gagagaatgc aaatctaaat aaaatagaac atgtactaaa
    30301 tgttttggat aaaagtttaa gtgcattagg attcaaagga aatggaaatt tgttaaattt
    30361 atttaataag tattattttt aatagatagg gggatatagt atggaagtta cagctaagca
    30421 aaaatttaca acgaaagaca tggtggaaac ctcactatta atagcattag tatttattgc
    30481 aactaaattt ataaacataa gattaccaat atctataaat ggtgggcttg tgcatttagg
    30541 aactgcaatg ttgttcatat cagcaatagt atttggaggt aaaaaaggtg ctttatcagg
    30601 ggctattggt atgtcacttt ttgatttgat atctgggtgg actttatggg caccgtttac
    30661 ctttatagtt agaggagtta tgggttattt acttggaaag attgcatggg caaatggtaa
    30721 taatgggaat aattttttaa tcaatgtaat aggaatatgt gtttcatcaa tttggatgct
    30781 atttggatat tatgtaacag aagttatact ttatggtaat tttatagtac ctcttacttc
    30841 aataccaggt aatttaatgc aagtattgat aggtcttata atagcgcttc ctatttctaa
    30901 ggtattgaaa aaatgtataa aataaatata aagtgaacta aaatttatga tagcataaat
    30961 tatactgtat aatattagga tggagtacat aattaaaaaa ttttagtata gaggttagtt
    31021 cacaatgtta tcagttacaa gatattcaat caaaaatgtt cgtttaaagc cgtgggtaaa
    31081 gtttatttgg tattttgaga caaaatcaaa tatactgtta aataataagt tacttccgac
    31141 agacagtata gatattattc tcaatctttc tgatgttatg gaatataaaa ttgaaaatca
    31201 agactatact gctagtaata tgcattttaa tggaataagg gataaacatg gatttattat
    31261 tcaacatgga aatatacgtg ttatagggat ttctttttat ccatttggtc tctatccatt
    31321 tttaaagata ccaatttcag agtttaacag acaaattgtt gacctagaag cagtatcaca
    31381 gctatttgca aaaaaattgg aagagtcatt aaatccaaca caatcagtag aaaaaattgt
    31441 tttatattta gaagaagtat tattatccat attagaagaa gatttaattt caaataaata
    31501 tgtaaagctt ctaaattcat ttatatatgt taatagatat agcaatataa aagcattttg
    31561 tgatgataca aatataaata ttaaaacatt ggaaagaatc tgtttaaaat atacagggta
    31621 tacacctaag attttaaaga gaatttacag atttaaaatg gcaagcaatc aacttattta
    31681 taattataaa gatgatgaac tttttgattt tatttatgaa aatgagtact atgaccaagc
    31741 acactttata aaagaattta aaaaattttc agggacgtca ccaattaaat ttattggtga
    31801 gaataagacg attaaagaaa atataaagta cagctattta taattatact gtcgattttt
    31861 tacaatacct ccaatttatt ttttgatatg atataggaaa ataaattgga ggttttacat
    31921 atgaaaatta ttacagaaat agttgagttt aatgtggaag aaaatttatc aaaagaagag
    31981 tttatagaaa ttgtaaatga cttagaatta aaatttcatt ctattcaatc tggttttatt
    32041 gatactgagc ttttatatga tgaaaagtct caaaagtgga ttatgataca acattgggaa
    32101 tctatagaag acctcaagag tgcttctagt aaaatgttta aagattcttc aactgagaaa
    32161 tttagaaaag ctttaaagaa tcaaactgtt aagatgaata taataccaca aattaaaagt
    32221 tggaaattat agaacttaat gtaaaagata gttaaatagt atcaaactaa attagccaaa
    32281 taacattttg tatttaaatt gttatttggc ttttagtgtt tattttaata tgagtgttat
    32341 atactagaaa aaccaacctt aaatactact atctaaaagc actattgctg tacgagaggc
    32401 aataggcatt ttagaagaag gaggagttga gctaaagtat acatcataat ctcttaagtt
    32461 ttcttttttt gtgtttcctc caaactctac catatcagta tttaatatcg ttttgtagtt
    32521 tgtattttga aactttgaaa tatcaactga ttgtattaga ttagggtgga aattaaatat
    32581 aaataaatat ttgcccttac tgtacaccaa aatcttttta tcattatcca tatgtataag
    32641 ttcatgacta ttttggataa aaatatcact gtattttgta agttcgagca tagatttatc
    32701 aaaatttaat aattgtttat actttaagtt gttatcttct gaaaggctcc attgtcttct
    32761 ggcatactta tagctccaat tgttgccttc tcgtggaaaa tcaatccact caggatgtcc
    32821 aaattcattt cccataaaat ttagatatcc ttcacctgct agagaaaaag ttattaattt
    32881 aattaattta ataagagata ttgctctatt gataacatgg ttatttgagt ttatttccat
    32941 attccagtac atttccttgt cagctagcca aaaaataatt gttttatcgc caactaatgc
    33001 ttggtcatgg gattcacaat atcctatatt tttttctcca ggtcgtctag tagtaagttc
    33061 ataccacatt tttcccaaat cccagtcttc atcagataag ttagagatag tttttatcca
    33121 aaaatcaggt actcccattg cgagtctata gtcaaatcca attccgccgt cttttatagg
    33181 gatacacata ccaggcattc cactcatatc ttctgcaata cttatcgaat taggtttaat
    33241 ttcttttatt aattcatttg caaattggag atatgttata gcttcaatat ctgtattcat
    33301 actaaaatat ttttcataac tatcaaatga aactccaaga ccatggttat gatataacat
    33361 tgaagtcaca ccatcaaacc taaagccatc aaaatggtac tcatttaacc aaaattttat
    33421 atttgaaagc aaaaaatgta ttacttcagg tttaccatag ttaaaaagtt ttgtacccca
    33481 agcaggatga tttccctttg aacctgagtg gaaaaattga tgttcacttc catcaaactc
    33541 atttattcct tctaaagtat tctttacagc atgagaatga actaaatcca ataaaacagc
    33601 aatccccatt gaatgagcag tgtttatgag gttttttaag tcttcaggag taccaaacct
    33661 agatgagatt gcataaaagt ttgaaacttg gtatccaaaa gatgcataat atggatgttc
    33721 cataatagcc ataagctgaa tggtattgta tccagctttt tttatcttag gaagtatttt
    33781 ttcagtaaat tcattataag ttccaataga ttcgctttca gtagacatac ctatatgaca
    33841 ttcatagatt agtggataag ttatgttttt taagtcaaaa tcattgtcag tccagataaa
    33901 tggagttttt ggttgccata tttgtccatt aaatccacca aaatcttttt gaattactct
    33961 ttttatatat aatggtatac ggtcaaatgt ttttccattt gcagtaactt gtacctttac
    34021 ctctgatttg tgaggtaagc tattttgacc aggaataaat atttcccaat taccagaccc
    34081 aatttttctt agaggatgag attttctatt ccaattatta aagtcaccaa ttagtgacaa
    34141 actatctgca tttggagccc attccctata gaagaatcca tcatttgtct gatgaaatcc
    34201 atagtaaaga tgagcatttg caaaagaact aaaatcttta tagtttttca atgtttttga
    34261 tttcatattc tcataatgtt tcattcgctt ttttatatca gattcaaatg gttgtaggta
    34321 tacatcaata tctaaaattt taaatctttt ttgacttttc attgtatttc tcctccataa
    34381 taaatattac atctaaaaaa taatatttat ctcatactat cttctctata taattgatat
    34441 atattatata atcctaataa aaagcttaag ccaccaatga ttaatttcat aatttacctc
    34501 cctagtattt atacttatat atcgtattca tcaaaattta aaagtttgtt taaaactctt
    34561 ttaataatat cataatcaaa gcctttatag gaaagatgtt gagaaatttt ttgatatatt
    34621 ttctttttat cttctttctt tactctttca tacctttttt ttgctagata catagcattt
    34681 tcaaattcag tgtccttgtc taattcagat actgcttcat tgattgtact tctattgata
    34741 cctttattat ataaattctg cttaattctg tttttgccac atttatttag gttaacattt
    34801 gtgttaacaa ttttttgagc taatacactg tcatttacga gattataact tttaagaaaa
    34861 tctatgaccc tctcaattat attttcatca aattctgatg atagtttttc ttttaatttt
    34921 ttttcagatt ggtcagcatg agataaaata tttaatgctt tatttttagc ttttatatac
    34981 atttcatcat ctaaaatagg ttttaattct tcttcattta tttcattccc cttttttaaa
    35041 ttaaatgtat aaacaagttc tttaaatata gcaataaaaa atttatcatc tacataaata
    35101 ttaactctat catcatttct tttttgttgt tctatttttg taattatacc cataagtcct
    35161 ccttttttat atgtaattat attaacaaaa gcaactaata aaatttaaca aaaaacttat
    35221 atttgtatgt tatattttat caaaattaag acaaaatagt aaaaagtt
//
LOCUS       DAMJVI010000032        34738 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.034, whole genome shotgun sequence.
ACCESSION   DAMJVI010000032 DAMJVI010000000
VERSION     DAMJVI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34738
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.034"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC545_001646"
     CDS             complement(441..2828)
                     /gene="cadA"
                     /locus_tag="PC545_001646"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDF2456417.1"
                     /translation="MEANNSIKKEFILGGLNCAHCAEEINNKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDTTIEKIIDIIDSTDPGLDIQISSKENAASKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTVNISNNFEEDDVINKIKEIINSTEPGLD
                     IQVGSTDKVKGRTTEKSGAVNDTNKKELIPLIIGALVYIFGIYQTATGYESQFSNIVF
                     IVAYVIVGGDVLLRAIRNISKGRVFDENFLMALATVGALTIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVNVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFVPPLVISGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGNFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIRSLKEIGVKEVVMLTGDNEKVAKNIAQELELDTVYSNLLPNEKVDRLEDLYEGRT
                     EKEKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKIIVMILGAGGVATMWEAIFADVGVALIAVVNAMRAMR"
     gene            complement(2851..3216)
                     /locus_tag="PC545_001647"
     CDS             complement(2851..3216)
                     /locus_tag="PC545_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456418.1"
                     /translation="MREEINDCNCNIVHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4263
                     /locus_tag="PC545_001648"
     CDS             3415..4263
                     /locus_tag="PC545_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456419.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQDELRNDPQISN
                     GYTKIPNNQNINNLYPNNVNHYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNN
                     NTGMPNNMSNNMNSNMGMPNNMPNNMNSNMGMPNNMPNNMNSNMGMPNNVPNNMNSNM
                     GMPNNMSNNMNSNMGMPNNMSGNMNNNMYGNTGMPNNMNSNMGYNMNNTMGIPNFSCN
                     QNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSNMSVQGIPQATNIEE
                     FDEEEM"
     gene            complement(4700..5788)
                     /locus_tag="PC545_001649"
     CDS             complement(4700..5788)
                     /locus_tag="PC545_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456420.1"
                     /translation="MNFNFAKATNSRLMGSLGLIINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLNNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYDKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYNKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNVVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSNMNEGIFF
                     TRFNFNNDHVKENVYIINNDMKAIYYLMGNKFFIGTYNENDCNYINEISQSNYSDYIK
                     FEEKFFFEQNALYDFAESGSLDFDDFLE"
     gene            5913..6641
                     /locus_tag="PC545_001650"
     CDS             5913..6641
                     /locus_tag="PC545_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2456421.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            complement(6843..7334)
                     /locus_tag="PC545_001651"
     CDS             complement(6843..7334)
                     /locus_tag="PC545_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456422.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIVLGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVYSSFIGYYNNRLRKML
                     FKK"
     gene            complement(7345..7788)
                     /locus_tag="PC545_001652"
     CDS             complement(7345..7788)
                     /locus_tag="PC545_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456423.1"
                     /translation="MYSNTYNNENFMRARSFIENSEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            complement(7778..8617)
                     /locus_tag="PC545_001653"
     CDS             complement(7778..8617)
                     /locus_tag="PC545_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456424.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDKRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFVYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYINILESRGGK
                     NVQ"
     gene            complement(8836..10221)
                     /locus_tag="PC545_001654"
     CDS             complement(8836..10221)
                     /locus_tag="PC545_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2456425.1"
                     /translation="MDNLNKKFLMEYIDEIQGELRDISDKIWKNPELQYKEYYASNLQ
                     KECLKKHGFSIEGLEGMDTAFVASYGEGRPVIAVLGEYDALDGLSQKVSTTKEPIVDG
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDNCRVHYVTTNGGKMPNIVPDFAEVWYYIRGVKMEHVRDVFGRIVDIA
                     KGAALMTGTIMEYDIISGVYDYIPNTILTDILSQNMKLVGVQNYNEDDYYFADKLAET
                     VSIDKRSSVSSVLSGNKDITKMNLHDEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSISCSINDVMLDELVINKAKKELKD
                     TVGSFKYIPII"
     gene            complement(10394..11137)
                     /locus_tag="PC545_001655"
     CDS             complement(10394..11137)
                     /locus_tag="PC545_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HDF2456426.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDCNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     IDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            complement(11157..12419)
                     /locus_tag="PC545_001656"
     CDS             complement(11157..12419)
                     /locus_tag="PC545_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2456427.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGTADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEQNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGVNAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNAHKILEETLGKKLEIKGSTAGNDARVMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            complement(12603..13601)
                     /locus_tag="PC545_001657"
     CDS             complement(12603..13601)
                     /locus_tag="PC545_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDF2456428.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSVFRDQGSAAVGLVIIFIIMSVAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWVGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYIGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKSAKSK"
     gene            complement(13585..15096)
                     /locus_tag="PC545_001658"
     CDS             complement(13585..15096)
                     /locus_tag="PC545_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDF2456429.1"
                     /translation="MSNIILKLSNIAKEFPGVRALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFEGKEIENISPDISKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDINPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLINDLKESGVSIIYISHRMEELFEICDRITIMRDGK
                     TISTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDISFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALKIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGDLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            complement(15150..16112)
                     /locus_tag="PC545_001659"
     CDS             complement(15150..16112)
                     /locus_tag="PC545_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDF2456430.1"
                     /translation="MRVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGGSDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC545_001660"
     CDS             complement(16160..17050)
                     /gene="rbsK"
                     /locus_tag="PC545_001660"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDF2456431.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCIGEDGLGEELIRNFRTNKVDYSLVSRNNHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRIRVL
                     LNPAPAVKLNEDIIEKVTYLTPNEHEYKIVFDTNEGIEEVLKKYPNKLVITEGKNGAR
                     FYDGEEIKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEDVEKYLNNK"
     gene            complement(17092..18105)
                     /locus_tag="PC545_001661"
     CDS             complement(17092..18105)
                     /locus_tag="PC545_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2456432.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANEEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKHLLDIGNKKIIYLSGPLQNEIAVDRLEGYKKALIECNLSYNPAY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDISVVGFD
                     DIQTSSLISPSLTTVKQPSYDIGYKASEILINCLRGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC545_001662"
     CDS             complement(18333..19310)
                     /gene="bioB"
                     /locus_tag="PC545_001662"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDF2456433.1"
                     /translation="MEKYIIKLKNKVLREKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREYFMGRKADLCTIMNAKSGKCSEDCKFCAQSSYYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNNILNIYEGLRENTNLKLCASLGIIDYEQAKMLK
                     SAGVTTYHHNVETCRDNFHNICTTHTYKDRIKTIKDAKKAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMENYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDKVGFNGGVNSAIVGDYLTTVGSGIENDKKMIIEQGFEL"
     gene            complement(19516..19779)
                     /locus_tag="PC545_001663"
     CDS             complement(19516..19779)
                     /locus_tag="PC545_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456434.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            complement(19849..20922)
                     /locus_tag="PC545_001664"
     CDS             complement(19849..20922)
                     /locus_tag="PC545_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2456435.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDEAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKDCEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCVGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKVGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            complement(21181..21939)
                     /locus_tag="PC545_001665"
     CDS             complement(21181..21939)
                     /locus_tag="PC545_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456436.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLIFGISSPISAKFMPD
                     LIAHFAPTLKVTAAPTALDSWTQFFSNISGLGMSLTLIIFCNILSNEYSKGTLVIMLT
                     KGLSRSSIVLSKFSVTVIIMTIGFWLSFLFAYGYTMYFWPTANLNHIIFSAFNLWLIG
                     IMYISVLILGCVLFRPAFASVLLVLVATLLLSLISIPKQIAPYTPNFLMSKNIDLISG
                     KVAMPEFIIPIIITVLISIVCLLAAVILFNKKPV"
     gene            complement(21936..22853)
                     /locus_tag="PC545_001666"
     CDS             complement(21936..22853)
                     /locus_tag="PC545_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456437.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLKPDSGTIDVCGEKVTYGKTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKKEIQIRSEELLSLVGLRNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSDGIKSLLKDSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            complement(22853..23053)
                     /locus_tag="PC545_001667"
     CDS             complement(22853..23053)
                     /locus_tag="PC545_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HDF2456438.1"
                     /translation="MENLMEYLPLLVPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISLIGPILYFTIGRGEE"
     gene            complement(23058..24152)
                     /locus_tag="PC545_001668"
     CDS             complement(23058..24152)
                     /locus_tag="PC545_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456439.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     PDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTVKPFDEVMDEIREI
                     IKKYYSCFPLIFRMGLLIVNHYDIVDEKKRELLISEALEIFIRIQETCNDIDICRQAK
                     SMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQMDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISDIFELDTLSPAIMLSSYLTQAQINLIH
                     GDNEGAIKSLRKYVDLATRDIYPIIIHGDDFFNKLDRWISEIGTGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            complement(24662..24802)
                     /locus_tag="PC545_001669"
     CDS             complement(24662..24802)
                     /locus_tag="PC545_001669"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HDF2456440.1"
                     /translation="MNGEGCILSNKITKECYKVGYMIREKSIDIYPDSGWGFMTWDEN
                     GE"
     gene            complement(25112..26419)
                     /locus_tag="PC545_001670"
     CDS             complement(25112..26419)
                     /locus_tag="PC545_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDF2456441.1"
                     /translation="MDISRSVDNSLNRTIEFLKELIKIDSQQGEPISQCPFGIGPKKS
                     LDKTLDYCASLGFSVKNIDNYIGYAEIGEGEELIGIPMHLDIVPPGEGWSVDPFSGAV
                     IDNIIYGRGVIDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMKCIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIIVSGGTKENVVPSHC
                     TAKIINGIISELTTKGVAVHASNPEKGENAISKMVIKIVEDNMDFQHREDIELVSKYL
                     CSDYYGDALGINQYDEVFKNTTLNLGILKVNEEKIVCELDIRYGKNIVLNNIIDRFKK
                     VFCNGWKIEVIVHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            complement(26484..26963)
                     /locus_tag="PC545_001671"
     CDS             complement(26484..26963)
                     /locus_tag="PC545_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HDF2456442.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGANPDDIC
                     KEAIYGFGKMRGQKYNVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQGVKLDFAQKCAHDDEVCELVFTKK"
     gene            complement(27010..27873)
                     /locus_tag="PC545_001672"
     CDS             complement(27010..27873)
                     /locus_tag="PC545_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2456443.1"
                     /translation="MQEIKNKEVKNGQGPTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKRKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIMFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLGVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            complement(27873..28628)
                     /locus_tag="PC545_001673"
     CDS             complement(27873..28628)
                     /locus_tag="PC545_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2456444.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLDVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            complement(28655..29143)
                     /locus_tag="PC545_001674"
     CDS             complement(28655..29143)
                     /locus_tag="PC545_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2456445.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGNEDVEQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            complement(29161..29562)
                     /locus_tag="PC545_001675"
     CDS             complement(29161..29562)
                     /locus_tag="PC545_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDF2456446.1"
                     /translation="MNKYKVIILCHGTWGKVLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKEVEEVVDKEDVKIIISDLYGGSTSSVAIAVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            complement(29565..30062)
                     /locus_tag="PC545_001676"
     CDS             complement(29565..30062)
                     /locus_tag="PC545_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2456447.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEVELNQNINRY
                     KGEFI"
     gene            complement(30086..30490)
                     /locus_tag="PC545_001677"
     CDS             complement(30086..30490)
                     /locus_tag="PC545_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2456448.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            complement(30720..33287)
                     /locus_tag="PC545_001678"
     CDS             complement(30720..33287)
                     /locus_tag="PC545_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2456449.1"
                     /translation="MTQKKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFDNINSINEYS
                     ENDSIKDEYILESIKKENNIFNLVGCDGSLRSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLTIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGVLFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESVRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKDFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDGDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYILQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANKIDKGKGILILTDMGSILTFAKVIRDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEVTYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRNKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            33453..34196
                     /locus_tag="PC545_001679"
     CDS             33453..34196
                     /locus_tag="PC545_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2456450.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIQNIPRIINNIIILRGCEANILDIKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNYPKKLINHFHQKGKLLDVDYTNI"
BASE COUNT        11261 a         6360 c         3560 g        13557 t
ORIGIN      
        1 cttaaaaata attcgtcaat actttttaaa attatttttt aaaaatattt actttcttat
       61 attacataat ttattgaatg gcagtcatac tgttggttta gagtatttta acacacacat
      121 tttgttgtaa aaattacaac tattaatata atatatatac aatcataagt attataattt
      181 ttatgaatct aatttaaact taatataaac ttattaataa accttctatg tctaaatttt
      241 aaaattacaa tataaaagct ctacactaaa taaatacatt aaataaacat aatggataat
      301 gaatataaat ttatatatgc aaagcgctcc aataaaaagg taccaataaa gttaaaagaa
      361 acttattgat acctttcatt attacatact tcaatatata attataagtt ttttaaatag
      421 tatggattta catttatccc ttatctcata gctctcatag catttactac tgctataagt
      481 gccacaccta catcagcaaa tatagcttcc cacatagtag ctactcctcc agcacctagt
      541 atcataacta taatttttac tcctagagca aatattatgt tttgccacac tatcttatta
      601 gtcttgtttg ctatttcaat tgctttagat atcttgctag gctcatctgt cataagcact
      661 acatctgcag cttcaatagc agcatctgaa cctaagcctc ccatagctat accaacatca
      721 gcacgagcta atactggagc atcatttatt ccatcaccta caaacgctat tttctctttt
      781 tcagttcttc cttcatataa atcttctaat ctatctacct tttcattagg aagtaaattt
      841 gagtaaactg tatccaattc taattcttga gcaatatttt ttgctacttt ctcattatct
      901 ccagttaaca ttacaacttc ttttacccct atttctttta agcttcttat ggcattttta
      961 ctatcttctt ttacttcatc agagataact atataacctc tatatacttt atcaacagca
     1021 atatagacta ctgtgcctac ttctttagct gatgaataag atatattttc ttttttcata
     1081 agtttctcat tacctgctaa tataaaattg ccattatgct ttactctaat tccataagct
     1141 gctatttcct cataactgtc tattgtatct aaatcaattt ttttattata gtaacttaat
     1201 atggactttg ctattggatg atttgaattt acctccgcaa tagctgcata ttctaataat
     1261 tcttcttctg atataccttc aggatttaac ttagttacat tgaacacacc tttagtaagt
     1321 gttcctgttt tatcaaatac tactgtatct acacttctta aagcttctaa atagttactt
     1381 cctttaataa ggataccatt tttagatgca aacccaattc cactaaaaaa actaagtggt
     1441 attgataaaa ctaatgcaca agggcaagaa acgactaaga atatcaatcc tctatataac
     1501 caatcactaa atacctcacc tgagattaca agtggtggca caaatgctat taataaagca
     1561 gctattacta ctattggagt atagtatctt gaaaacttag atataaagtt ttctgtcttt
     1621 gattttttaa tgctagaatt ttcaacaagg tcaagaatct tagaaactgt tgattctcca
     1681 aaactctttg taacttcaat actcaataaa gcatttttat ttataacacc tgataagatt
     1741 tcatctcctt tttcaacttc tctcagcact gactctcctg tgagtgcaga agtatctaac
     1801 atagaaactc catctactac tacaccatct aatggtactt tttcaccagg ttttactact
     1861 attatatcac ctacatttac ctcttctggt gacaccacct taacttcaga attaactttt
     1921 agatttgcat agtctggtct tatttgcata agtgaagtta tagactttct ggatttacca
     1981 actgcaacac cttgtaaata ctctcctact ttatagaaaa gcataacccc tactgcttct
     2041 gataactccc ctattgttaa tgctcctact gttgctagag ccatcaaaaa gttctcatca
     2101 aatactcttc cttttgatat atttcttatt gctcttaaaa gcacatcacc acctactatt
     2161 acataggcaa ctataaatac tatattgcta aactgacttt cataacctgt agctgtttga
     2221 tatataccaa atatatatac aagtgctcca attataagtg gaattaattc tttcttattt
     2281 gtatcattta cagctcctga tttttcagta gttctccctt ttactttgtc agtagaccct
     2341 acttgtatat caagccctgg ctctgttgaa tttattattt cttttatttt atttataaca
     2401 tcatcctctt caaagttatt acttatattt actgtaagtt ttttatttac aaagtttaaa
     2461 ttagaagatt caacttcttt taatttagaa actttgttat taatctcttc tgcacaatga
     2521 gcacagttta atcctcctaa tataagctct tttttaattg atgttttact agctgcattt
     2581 tctttagaac ttatttgtat atcaagacct ggatctgttg aatctattat atctattatc
     2641 ttttctatag tagtatcctc attaaaactt tcctttatat ttactgtaag ttttttattt
     2701 ataaaattta aattggaaga tttaacttct tgtagtttag aaactttatt attaatctcc
     2761 tctgcacagt gagcacaatt taatcctcct agtataaatt cttttttaat tgaattattt
     2821 gcttccatat atattcactc ctctcattta tcatttataa gtctctctaa tatgatttaa
     2881 tccacaatca aatatctgac ttatatgact atcatctaat gagtaataca ctgtttttcc
     2941 ttctcttcta aactttacta atctagcctg ctttaatact ctaagttgat gagatattgc
     3001 agaatgagtc atatttaata aactcgctat atcacataca cacatttcat ttgcaaataa
     3061 tgcatataat atctttactc tagtagtatc cccaaatact ttaaataatt ctgctaaatc
     3121 atataacatt tcttcatctg gcatagttga tttagcctct gttactattt cttcatgtac
     3181 tatattacaa ttacaatcat ttatctcttc tctcattttt aatcctccat tattttaata
     3241 tatgaataac tgttcatatg ttcattatat gttatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatataaat agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcgaactat
     3481 gcagattata ttactaatga agaaattccc caagatgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaaat acctaataac cagaacataa ataacctata tcctaataat
     3601 gtaaatcatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tccggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaataat
     3721 aatacgggta tgcctaataa tatgtctaac aatatgaata gtaatatggg tatgcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtatgccta acaatatgcc taataatatg
     3841 aatagtaaca tgggcatgcc taacaatgtg cctaataaca tgaatagtaa tatgggtatg
     3901 cctaacaata tgtctaataa tatgaatagt aatatgggca tgcctaacaa tatgtctggc
     3961 aatatgaata acaatatgta tggtaataca ggtatgccta ataacatgaa cagtaatatg
     4021 gggtataata tgaacaatac tatgggtatt cctaacttct catgtaatca aaatatgcct
     4081 cctaatgtac ttatgatgat accaggagtt atatgtcata atacaatgca aggtatgcct
     4141 gtagttatgc ctagtactat gcctcctaat atatatccta ctccttatgg ttctagtaat
     4201 atgtctgtac aaggtatacc acaagctact aatattgagg aatttgatga ggaagaaatg
     4261 taatatatat ttttttaagt aagttgtata aaacattaat aattgaccat aataataaat
     4321 gtattcctca taatattccc ccaatattat tataggtata gaaagttgac tgaatatgcg
     4381 cttattcggt caacagatat taacataatc taataagata aacatagaaa aagtcagata
     4441 aaatttaatt ttatctgact ttttcattta aaaatgctct cttgtgttgg tgattcagaa
     4501 tagtatggta ctcgtatttg agactaatat tggcaaacaa cataaagaaa aatataaatt
     4561 ttttataata aactgtataa aattttaata attgaccaca ataataaatg tattcctcat
     4621 aatattcccc caatattata gaaataaatt taagcgagat taaattcagt taatctcgct
     4681 taaataatgt ctaaatttat tattccaaaa aatcatcaaa atctaatgaa cctgattctg
     4741 caaaatcata taatgcattt tgctcaaaga aaaatttctc ttcaaattta atatagtcgc
     4801 tataattaga ttgacttatt tcattgatat aattgcaatc attttcgtta taagtaccaa
     4861 taaaaaactt atttcccatt aagtaatata tagctttcat atcattattt attatatata
     4921 cattttcttt tacatggtca ttattaaaat taaatctagt aaagaatata ccttcattca
     4981 tattactttt taacagaaat ggattttctc tataaaatac tcctgcaaat tcttcaggtg
     5041 aatttacact gtaaacactt acaaattcac ttttagaaat ctcatcaaat acctcaaaat
     5101 catacatagg ttctttatct gtcactaata gagaattaat tctaaaacca gtttcataac
     5161 acttacttat gcaaaaagca attttacata tataatatcc atcattatct tcatataaag
     5221 cttcacttat atatctgcct tgacctttat cacttaccac gtttttaagt aatgttccat
     5281 ttatattagt aatgtgcata tttgcaatct cgtcacttcc agaaaagtac tttaaacttt
     5341 ctctatccca tgctataaat ctcatggtca tatagtttat aaattcaact tcctcgtcaa
     5401 ctcttttaca tattttattg tataattttt ctatagttaa atctgtttta tagtttttta
     5461 taatatcaat atactcacat ttatctcctg gcaatagctt atcataataa agattcttat
     5521 taccaaaata agatactaaa aataaagatt catccttcgt taattcaact ctatctgaac
     5581 caaatcctcc catcaatctt tcttcttcca tataagcttc ttcttgtgtt gggttattta
     5641 aactcacata atccgctaat cctaatccct ctgcatctaa taaaaaatat tgacaaaagt
     5701 gattgttttc atcatctatc caatttatta taagacctaa actgcccata agtctagaat
     5761 ttgtagcctt tgcaaaatta aaattcatat agtcacatcc tttaaactct aaaatacttg
     5821 ttttttaata tataaattat tatacatata ttttaacaac ttagatacaa tttataatat
     5881 aatataagga aatgattaat tgaggtggtc ttatgaaaaa tatagcagct ttttttgata
     5941 tagatggtac tctttataga gactctctta tggttgaaca ttttaaaaaa ttaattaaat
     6001 acgatataat tgaccaaaaa aattggtata aacatgctag agacactttt atggattggg
     6061 ataaaagaca aggtaattat gatgattatc tattagaaat atgtgacctt tatgttgatt
     6121 cactaaaagg attagataaa acctgtatca actttacaag tgaccaagta attaaattaa
     6181 aagcagatag agtttataaa tacacacgtt caaggattga gtggcatcta aatcaaggac
     6241 acatagttat atttatatct ggtagccctg gatttcttgt tgaaaaaatg gcaaaaaaat
     6301 ataatgtaac cgattttcta ggaagtgatt atgtctttga aaacaatata tttacaggca
     6361 cagttattcc aatgtgggat tctataagta agaataatgc tataaacgat tttgttgtaa
     6421 agtatgattt agatttatca aactcgtatg cctatggaga tactaatggt gatataaata
     6481 tgctaaaaag agttggaaat ccaatagcaa taaatccaac aaaggaactt ttatctcaaa
     6541 tatcacttga tgaggaaatt tctaaaaatg ttgagataat agtagaaaga aaagatatta
     6601 tttattctct atctacagat gtaaatatag tggatattta atttatacta tctattttaa
     6661 aaacttatat tatatctaat atttatatct aacatataaa agctttgact aaaaatcaaa
     6721 gcttttatat gttgcactaa gaatatatta aatttagctt tatgctttaa tgtatattca
     6781 tatatatcat ttacacttat ctaaactaca atttcattaa aattaaatat aaaaatcaac
     6841 tattattttt taaatagcat ctttcttaat ctattattgt aataacctat aaaagaactg
     6901 taaacatagt catatactat aaaggctatc tcaaatgaac ctataataaa tatgtttatt
     6961 ggaatataaa ctattgttct taataaaaaa tacaatataa gtatcattat atttgcaaat
     7021 actaatttca taaaatactc aatatatata gacctatctt tttctataag atattttaca
     7081 ataccatata ttccaaaggt aagtgaatat acaatccatt gagacttact tgccatcact
     7141 ataaatccta aaactataga acctatataa aaagcaattc cactcttaaa gccccattcc
     7201 attattacaa ttgatactaa aagtgatgct aaccccataa taaacaatgt atttacagga
     7261 ataatgtttg taagagtcaa caatatcaca tttaatgcta ataaaactcc tccataagca
     7321 acttttatac tcatatttat catcctaaca acaagttatt aaatctccac caaaacattc
     7381 acaacatgaa tctaaacacc aaagtttagc acaattttca caacaatcgt cacctagaca
     7441 acaacagcaa tcatcacagc aacagcaaca acatccatct aaatcattac gtcttcttct
     7501 attataattg tcagccctat tgctataatc atttctatat tgtgtataac ttctaagtgc
     7561 atcactatat tcactatttt caggctccat aaattttgct ctttttaaga agtcttcacc
     7621 ttcttcataa tatcctatgt tcattgcaga aaaaccagtt aaataatacc actctgcaca
     7681 tttgtcagtc aaagttttta aaaaatcata tgctttttta aattcactat tttctatgaa
     7741 tgatcttgct ctcataaaat tttcattatt ataagtatta ctgtacattc tttccacctc
     7801 tactttctaa tatattaata tatcttaaat aaacaccaga ataaattata ttttctatta
     7861 ttccaacatt tttatttaga tttagattat caattcccct agctaaaaat cctaatgatg
     7921 ttactattat tttttttact ttttctttta actcatcatt tttatcaata tactctataa
     7981 atggattata tcttcctttc ttaaaatctt catctaaatc ttcataggca tctagtagat
     8041 atatatactt acctatatta aaacctatca ttcttaagtt tctctcattt tcatcttttt
     8101 tatagacaaa tacttcaccc attatatttc caaatatact agatacttca tctatattta
     8161 tatttttatc tttttctaac ttatttaatt catttagttg ttgttttata tactctgctt
     8221 tttcaggata cttttcatat gctaatttta atttgttttt atatatataa tatgccaatt
     8281 tatcttttat gcgtttatca tccattaaat tatcttccaa tttataatac gttaaaagaa
     8341 tattcatact tgctgcatat tctgtaatat catttgtaat tacctttttc tttttaaatg
     8401 gatttactat acacacttcc tctgtactta tagactctgg gttatatact gctgttaata
     8461 aaacaattaa aaatgttata tcatagttca aagaaaacct agagatttct ccatgatttc
     8521 tttttaagta tttacatagt ccacaatagt aagctttgta ataatcatat tctctaaatg
     8581 ttaaatccat tttgtttatt ttaacatatc caaacataaa tactcctttt tgtactattt
     8641 tttatttaga ttatatatag tataacactt tttatcaaga ttatataatt tacagtattc
     8701 taagaaactt aaaaaattcc ttctaatcat tgtatcttaa atttaaaata attagaaggt
     8761 tgggttaaaa ataatattaa attatatact tatattaact catacaagtt agttatgctt
     8821 atatcaattt aagttttaaa taattggtat atatttaaaa ctacctacag tatccttcaa
     8881 ttcttttttt gctttgttta taaccaattc atcaagcatt acatcattta ttgaacatga
     8941 tatagattta gatgccaata acattgcctt aaatcccata tcagaacctg cactggcaca
     9001 agattgccat gtatgggcag aaataccaat aggccaaact gaacaagaac actgtgctgt
     9061 aggaattatg tagctaacat ctccaatatc taatgataat gatatacagt tattatgagt
     9121 aaatgtatca tccgtaactt catcatgcag attcatctta gtaatatcct tatttccact
     9181 taatacacta gaaacactac ttcttttatc tatagacact gtctcagcta atttatctgc
     9241 aaaatagtag tcatcttcat tatagttttg cacacctact aatttcatat tttgtgatag
     9301 aatatctgtt aatattgtat ttggaatata gtcatatact ccacttataa tgtcatattc
     9361 cataatagta ccagtcatta atgctgctcc ttttgctata tcaacaatcc taccaaatac
     9421 atctcttaca tgctccattt taactcctct tatgtaatac cacacttctg caaaatcagg
     9481 aactatattt ggcatttttc caccattagt agttacatag tgtactctac aattatcaaa
     9541 tatatgttct ctcaaataat tacaacctat attcataagt tctactgcat caagtgcact
     9601 tcttccgtta taaggagctt gtgctgcatg ggcactaatc ccattatatc taaatttcac
     9661 agagtaatta gcaagtgttg gtattcttat tggagtattt atatcaaatg gatgccaact
     9721 aaaagcacaa tcaattccat caaaaaaacc tttttttatc atcaatactt ttccagataa
     9781 atcttcttct gctggacatc catagtattt tatagtccct ttcagctttt ttagtttgat
     9841 taattccttt actgcaatta cagctcctat tgaacctgta ccaagtaagt tatgtccaca
     9901 tccatgacct gcaccaccat ctacaatagg ttctttagta gtggatactt tttgagacaa
     9961 accatctaaa gcatcatatt ctcccaaaac tgctataact ggtctacctt ctccataact
    10021 agccacaaat gctgtatcca taccttctaa tccctctata ctaaaaccat gtttttttaa
    10081 acattctttt tgtaaatttg aagcatagta ttctttatac tgaagttctg gatttttcca
    10141 tatcttatct gatatatctc ttaattctcc ctgtatctca tctatatatt ccatcaaaaa
    10201 ctttttattt aaattatcca tattaattat ataaacctcc ctgtttctaa taacttatcg
    10261 attatcagcc aaaatgacta attttaactg atttaaaaat attttctacc atacaaccat
    10321 tttatacaat aaaacctctt gacttgtaag tttcatttta tcaagtaaga ggttttcaag
    10381 ttttataaaa tttttagcat gttaaccaat caagcatatt aacccataat ttattatagt
    10441 atttccaatc agtaaattct tttggacccc agtgaggtgc acagtcagaa ctaaagatag
    10501 cagattttcc ttttccaaac tcgccaacag ctacaaatgg gtctcctcca atagttgcta
    10561 aaacaggaca attatctcta gctactgttt tgttatatcc taaaaacttt ggccattctg
    10621 ttggtatacc ttttaatact ggatgttcag agtcaataac ttctggaatt attcctgctg
    10681 gtttttcaac tctatcatct ttatctatca ttgttattgg taatacatct ttgatagcag
    10741 tttctccaaa tctagctttt gcatctattc ctgtaaatga catatatcca cctaccatga
    10801 ttaacgcccc accattattt acatactctt tgattagttc aagtctatct ggattactat
    10861 tgcaatctat aaaagtacta ttagataata agaacgtatt agaacctata tcagataata
    10921 ttatacagtc atactcattt aactcttcta atttatacgg aaaactatct gctgctacat
    10981 gtgcaggcat atgaactgtc tcatatcctc cagattctat tgcttctttc aaccacttta
    11041 cagcttcttc ataatgagtt gtaacaaacg tatcaaaccc ttttatatga gttatatttt
    11101 ttacccaaga ttcccctact attaatactt ttttcattta tatattcctc ctacttttat
    11161 aagtttaata ttagacttgc atacataagt atactatcta aatactcctt aatttcaaca
    11221 tattcatcaa ttgaatgaca ttgttctatt gaacctggcc ctaatataag tgttggtatt
    11281 tcagctaaat ttttcattac tctcgcatca ttgccagctg ttgaaccttt tatctctaat
    11341 ttttttccta gagtttcttc tagtatttta tgtgcattca ctacaaaatc agaatccttg
    11401 tccatctcaa aacctaaccc tgattgatat atgtttatat ttggtttatt atctttaagc
    11461 cataaatctc cattagccct tgtcattatg acatttgtta catcatttac aacttgttca
    11521 taactcatta tatttggcaa gaagtgtaca cataaattaa atacacactt atctggcaca
    11581 gtagaacctg ctgttccacc atttattaca cctaaattta ttgtaggtga aggtaatagt
    11641 ggatgtttat atatcataag ccagttatgt tccaactctt ttatatcttg aagcaacaac
    11701 atagccttct caattgcatt tacaccctcc cacttactac cacagtgaag agacacacct
    11761 ttaacctcaa cttcaaagaa tacaaatccc atatgtgcaa ctattaagtt ttgttctgat
    11821 ggttcacaaa ttatgcaaca atctccatca ataccattca taactgcatt tatggtacca
    11881 tttccgccac cttcttcatc tactactgac attattttta cattaccagg tacatttaga
    11941 ccactatctt ttataagctt tacagctaat attgatgcta taagtccaga tttcatatca
    12001 gcagttccaa gaccataaag ttttccatta tcttctgtag cacgatatgg attatatttc
    12061 cacttactta tatcacccgg tggcatagtg tctacatgac cattaaatac aattgtctta
    12121 ccaggtaaat catcactata cttacaaatt aaattatatc tatcttgata attgtgacct
    12181 agatttcctt ctttatatat attttttgct tttttaatta attcttcgct catttcttgt
    12241 ctatccacac taaaacctat gtaatttgca agtttttcaa tatattcctg tccttctttt
    12301 tcaaatccac ctagtattcc atgaccaaca tcttcagtct ttatacttac aagttctttt
    12361 aaataatcta tgtattcttg ttttttacta tttaatgtat ctaagagaat ttttttcata
    12421 tttcctccta agatttattt ttacatttaa aatttcaagt ctaatctcat ctatttaagc
    12481 catagtaaac tttttcactt agcatattct cattcaatgt ataaagatgt atgcaaatta
    12541 aagtataaaa atatatacaa aaacttaata atttttatag tttataagct aaaatttaat
    12601 tactatttag atttagctga tttttttaat tttatattat ttatccatat agcaaataac
    12661 attaccaaac cttttataat catttgatta tatgaagata cacttaataa gtttaatcca
    12721 tttcctaata ttcctattat tccgacacct atacatgtac ctactataga accagaacca
    12781 cccatcatat tagtaccacc aagcacaacg gctgctatag catccaattc tgctccatct
    12841 ccaacagttg gtgaacctga tgccaaacgt gatgatagta ttatcccagc taatgctgtt
    12901 agtagtccag ctagtgcgta tacttttact ataactttat ttacttttac tccacataat
    12961 ctagcggcct cttcatttcc acctactgca tgtacatgac gtccaaaatt agttcttttt
    13021 aatagaaaat gtcctgctat tacaactata atcataatta taactggtat tggtattggt
    13081 ccaatatatc ctctaccgag catcttaaat gcatctgtat ttactgatat aggtcttcct
    13141 cctgtaaata caaagcttgc cccacttatt atcgttaaca tggctaaagt tacaatgaat
    13201 ggaggtagtt tttgtgttga aataattgca ccttttatga ctcctaatat tgtccctatt
    13261 gctaatgcaa gtatcatacc tacccatata ggtgcattat aattcactac tgttgtagcc
    13321 caaaatgtac ctactaaagc aattactgct cctacactta aatctattcc tcctgtaatt
    13381 attacaaatg tcatacctac tgcaagtatt gcatttattg atatttgtct tccaacatta
    13441 attaaattat ctaaagttaa aaagttagat gaagctacac tcattattat aaaaattatt
    13501 acaagtccta cagctgcact cccttggtct ctaaatacac ttgttaattt tgtcaataat
    13561 ggagaagatt tttcttcttt atgcttagtt attgaactca ttaattttac ctccgaaagc
    13621 caattttaat acattatttt caaccatttc ttcaccttct aaaaatccac ttatatgacc
    13681 ttctctcata accattattc tgtcactcat tgatattaat tctggtattt cagaagatat
    13741 cattattata cttacaccac tctccactaa atctcccatt agattgtata tctctgcctt
    13801 agcatttaca tctatacctc tggttggttc atccataatc aatatatctg gttgtaataa
    13861 aagccatctt gctattatag ctttttgttg gtttccacca cttaaacttt gtataacttg
    13921 attcatatgt ggtgttttta tctttaaagc ttctatgtat ttttctaaaa tctctttttc
    13981 cttttttcta tctataattg atttaaactt agatatcttt tttaacccag ccatagttgt
    14041 attttctttg acactcattt gtaaaaacaa tccagtacct tttctatctt ctgttattaa
    14101 tcctagtcca ttttctattg catctgatgg agttttaaac tttacttctt cattgtttaa
    14161 atatattttt ccagaatcat atgttctaag tccaaatata gcttctacta tttcacttct
    14221 cttacttcca actagtccac ctattcccaa tatttcacct tttcttatat taaagctaat
    14281 atcattaaaa acattgttct tagttagatt ttccactcgc ataatttctt cttttatttc
    14341 tacttttctt tttgggaaaa attggtctag ctttctatct atcattaaat taactaattc
    14401 ttcctcagac gatacatctt tggtatttaa agtcgaaatc gttttcccat ctcgcattat
    14461 agttattctg tcacaaatct caaagagttc ttccatccta tgggatatat atattatact
    14521 aactccactc tcttttaaat catttataag tctaaaaagt atttcagtct cttttttact
    14581 tattgaagaa gtaggttcat ccattattaa tactttagtt tctgatgata aaactcttgc
    14641 tatttctacc atttgttttt gagctatact aagctctttg actaaactat ttggatttat
    14701 atctattccc atattatcga aaatcttttt agcctcttca ttcatttttt tgtcattggt
    14761 taagactttg ccatttattt cttttcccat aaaaatattt tgtgctactg ttaattcgtc
    14821 aaaaactgtt agttcttgat atatggcatt tatacctatc tttttagaga tatctgggga
    14881 tatattctct atttctttgc cttcaaaaat aaattttcca gaagttttcg aatgtgcacc
    14941 agttaaaatc tttatcattg ttgatttacc agcaccattt tcacccagta gtgcatgaac
    15001 ttcgccatga aataactcaa aattaacatt atctaatgct ctgacaccag gaaattcttt
    15061 agcaatatta cttaacttca gaattatatt actcataatg acactccttt ttaaatagga
    15121 tttctttcaa ataatctcat tttattttac taattcttaa agtcttttat attttcctta
    15181 tcataaagta aagctggtac tggtatattc ttttctacct tttccccttt tatagctttt
    15241 actactgctt ctacagctaa acgtcccatc tctttaggtt gttgttgaat catagcaaga
    15301 atagttccat ctgttattcc acttgatgct tcttcagtta agtcaaatcc tacaatctta
    15361 acatccttat tttttgcaga ttcaagtgca gctttagctc ctagtacact attttcagct
    15421 gttgcatata ttatatctac atctggatta gattgaatta agttttctac tgtattaaga
    15481 gattcttctc tgtcatatcc tggttgtgaa tttaatatct taacatttgc tgaacctttt
    15541 aatgcatctt taaatccatc tattctctgc atttgtattt gagattttaa atctgtcact
    15601 attgctattt ctgactttcc acctaacttt tcatctatgt attttttagt gtattcccct
    15661 aattttttac cagcatcata actaactgtt cctacataac ttgtaatatt ttcagtattt
    15721 aatacagcat ctactgtaac tactggcatt ttttcaaatg ctccttctac tgctggtact
    15781 atagccgaat tatcagttcc aagtactatc attccatcta cattttgttg tataaaatct
    15841 tctattgctg atgtttgctt agctacatct tggtctgggt catttactat aactttcaca
    15901 cctaattctt tagctttttc ttttattccc tcatcaatat ctataaaaaa ttgatgttga
    15961 agagttattg tagatactcc aatagttaaa tctttcttgt ctgaaccacc tttattatct
    16021 gatttacttg gtccttccat tgaacaccct gttaacattc ctattgctag tattccagca
    16081 attcctaaag ataaaatttt tctcactctc ataattaacc tccattttta atagttttaa
    16141 atttgaacaa attagatttt tatttattat ttaaatattt ttcaacatct tctttataag
    16201 gcataccaga ttgtgcacct agtttagtta cagacagacc tgatactact actgcatatt
    16261 ctactgctgt atataaattt ttcccttctg ttatagccac tgccaatgct ccattaaatg
    16321 tatctcctgc tcctgtggta tcttgaacat caacacttat acaagataca tgcttgattt
    16381 cctcaccatc ataaaatcta gctccatttt ttccttctgt tattacaagt ttatttggat
    16441 atttttttaa tacttcttct atcccttcat ttgtgtcaaa aactatctta tattcatgtt
    16501 catttggtgt taagtaagtt actttttcta ttatatcttc atttagtttt actgctggtg
    16561 ctggatttaa taaaacccta atcctatttt caaaacagaa attcactaca taatttattg
    16621 tttttaatgg aatctctaat tgtagcaata ctatatctgc attctttatc tcttcttcat
    16681 tctttttaat taattctata tctactagct cattagtccc tggtacaaca acaatactat
    16741 tatcattttc acataatgtt ataacagcaa caccagaact tttgtgatta tttctggata
    16801 ctaagctata atcaacttta ttcgttctaa aatttcttat taactcctct cctaagccat
    16861 cttcccctat gcatgatatc atttttacat ttgctcctag tttagaagct gagatagctt
    16921 gattagcccc ttttcctcca ggtgatgtaa aaaaactatt acctaatact gtttctcctt
    16981 tttctggtct tttatctaca gaacatacca tgtccatgtt tacacttcct atgacaacta
    17041 tgtttcccat tacattactc ctttacatat ctatttatgt ctctcgtgct gttattcact
    17101 ttcttttgtt gaatctctta tgaccaattc tggtttgaaa atcaattcat caaacgattc
    17161 ttttttatct cctcttaaac aatttatcaa tatttcactg gctttatatc ctatatcata
    17221 agacggctgt tttactgtgg taagagaagg gcttattaga cttgatgttt gaatatcatc
    17281 aaatcccacg acacttatat catttggtat acttaatcct ctttctttaa gacctcttat
    17341 gacacctatt gcaatcaaat catttgcaca aaatattgca tcaaaatcta tcttttctaa
    17401 attttttata aattcttgtc cccattcaat cttatatttt ccttcaaata cataagcagg
    17461 attgtagctt aaattgcact cgattagagc ctttttatat ccttctaatc tatctactgc
    17521 tatttcattt tgaagtggac cacttaggta tattattttc ttatttccaa tatctaataa
    17581 atgttttgta gctatatagg ccccttcaaa gttatttatt ttaacaattc cttttaaggt
    17641 ttctttgttc atacttatct tcttatcaat caagacagtt ggtattttta tatctttata
    17701 atttgcttct ttccagtttg tatttgaaga tgctgcaaat ataattccat ctatcatcct
    17761 ctctgatagt gttcttattc cttcatattc ttttttagca tcttcatctg tattacataa
    17821 aattatattg tagccttctt cattagcttt gtcttctaca cctcttgata catctgtaaa
    17881 gaatggattt cgtatatctg gtataagaag ccctatagta ttagtttttt tagttactaa
    17941 actttgtgct aatttatttg gtacataatt tttttccttc attattttca aaattttttc
    18001 tcttgttgcc tgacttatat tttcatcttt attattaatg acttttgaca cagttgtttt
    18061 agaaacacca gccaattcgc caatttcttt tatagttata ctcaagaagt tcacccccaa
    18121 aatttaggaa accgttttac ttaaccggtt acctagatac tatcacatct tttttccatt
    18181 tgcaaccatt ttatataaat tttgatattt ttcccttttt tcaaaatatt gcaacttata
    18241 attcaatttt aatatttaaa ttaatctgaa aaaaaaatta atatactttt ataattatct
    18301 aaaagtatat taattaattt aagcagtatt tattaaagtt caaatccttg ctctataatc
    18361 atttttttat cattttcaat cccacttcca acagttgtca aataatcacc aacaattgca
    18421 gaatttactc ccccattaaa tccaactttg tcatatccct ttaatgatag ccttccacca
    18481 gcatacctta tgtacacatt aggaattata aacctaaata ctgctgttgt ttttagtatt
    18541 tccattggtt ctaatacatc ataattttcc attggtgtat tcttaatagg atttaaaatg
    18601 tttattggaa atgattttac atttaattct cgtatttcaa aagccatttt tagtctctgt
    18661 tcttcactct cattcattcc aattataccg cctacacata catctagccc agctttttta
    18721 gcatctttta tagtttttat cctatcttta tatgtgtgtg ttgtacatat attatgaaaa
    18781 ttatctctac acgtctcaac attgtggtga tatgtagtca cacctgctga cttcaacatt
    18841 ttagcttgct cataatctat aatacctaaa gatgcacata attttaaatt agtattttcc
    18901 ctcaatcctt catagatatt taaaatatta ttaaactcct ttcctgacat acctttacca
    18961 cttgtaacca atgagaatct atgcactcct ttactctcca tctctttagc tctatttaag
    19021 atttcattgt aatctaataa tgaatattct tctacaccag ttttataata agaagactga
    19081 gcacaaaatt tacagtcttc actacatttg ccagatttag cattcattat cgtacataaa
    19141 tctgcttttc ttcccataaa atattctctt atttcatttg cagattttaa tagaatatca
    19201 aaatcgtttt tattatttgt atcaaggctt attaaattaa gagcttcttc ataacttatt
    19261 tctttttctc tcaatacttt attttttaat tttattatgt atttttccat atatcctcct
    19321 atttgtcaac ttaatatttt tatgtagttt acttattata tatagtattg catatataac
    19381 atttattggt caactttatt ttagacacaa gtttactata cacaaaaaaa tactgcttca
    19441 aaacaatatt agtatataga ttatacatat tattattaaa gcagtatttt ttattagaat
    19501 ataattctta ctggtttata aaactatcct tgtcttaact ccatccaagc tttccattgt
    19561 aataacttct aagccttcat attctctaaa tagccatttt aaatccttca tggcttgatt
    19621 aacctgactt tttgttagat tatttataaa atattctttt ctagtttcct catactcttt
    19681 gctagtcaaa aagtttctta gaaaattaaa cgcaaataca cttggaatat tgtttacatt
    19741 agccttcttg ctgttttcct tctcatagta aattttcata aatgcatact cctttgatac
    19801 ttttagattt ctcttacaat aattatatgc atttaagtgc tactattctt ataattttat
    19861 taattcatat tctttacttc ccatacccat ttcacatgca tagtctatta ttctagttcc
    19921 atttacatga ggatgttcat gtttgaaaac atcatgaccc atttccttac aaatcaaatc
    19981 atagcatgct tgttcaatag ctacagggtc tgttgatgct aatattccta tatcatgggc
    20041 aattggtcta cctgaccaag gtacacaatc acaaagtgga gttacattca taacaaaagt
    20101 tatataccct accttgtctt ttttatttga tactgctcca taagcgtatt ctgccatctt
    20161 ttcgacaaac acatctgagt cactttccca ttgtatagtt actgctctag ttggacagac
    20221 agttggacat tctccacatc cataacatac atcagaatct ataactgctt ttttatccac
    20281 tatacttata gcttttgtag gacaagaatt aacacacttt ccacaaccta cacacttctt
    20341 ttcctttact actggagtaa catcagaatg ttgcatttgt tttcctgctg cacttgcaca
    20401 tcccatagct aagtttttta atgctcctcc aaaacctgct gcttcatgac ctttaaaatg
    20461 actcattact atcattgcag aagaattata aatttctcca cctattttta cactttcaaa
    20521 atgttttttg tctattttta cattctcata atttctacta tataatccat ctgcaattat
    20581 tactggtgca tttacaactg cataagcaaa tccattttct atagcagttg taagatggtc
    20641 tacagaattt gttctactcc ctgtatacag tgtatttgta tctgttaaaa atggctttcc
    20701 ttcgcagtct ttaaccttat caaccacttg tctaactgcc acaggactca tatatgtagt
    20761 atttcccttt tctccaaagt gaagtttaat tgcgacttgg tcattttttt ctattaaatc
    20821 tttgaatcct gcttcatcaa ataatcttct tacattgttt ggtacgtttt tattttgcga
    20881 atttgagtat aaatcacaaa aatatacttt tgacttttcc atatctacac ccctttaaaa
    20941 tatttactta tactaatttt atcactttta aagtaatttt tagtatattt taacataatt
    21001 aaatattctt atatttattt gatacaaata atattatttg tattattttc tacacttaat
    21061 tctatatatt aaactacacc taaaaatcaa aaagacttga aacttaagtc caagtctttt
    21121 tgatttttag gtgtagtttt taacaacata agattacttt accttgtgat aaaggtatta
    21181 ttaaactggt tttttattaa ataaaataac agctgctagt aaacatacta tagaaataag
    21241 aactgttata ataattggaa taataaattc tggcattgcc actttccctg aaatcaagtc
    21301 tatatttttt gacatcaaga aattaggagt ataaggtgca atctgtttag gaatagaaat
    21361 taaactcaat aagagagttg ctaccaatac aagtaaaaca ctggcaaatg ctggtctaaa
    21421 tagtacacac ccaagaatta atacactaat atacataatt cctataagcc ataaattaaa
    21481 tgctgaaaat ataatatgat taagatttgc agttggccaa aaatacattg tatagccata
    21541 agcaaataaa aaactcaacc aaaaaccaat tgtcataata atcaccgtaa cagagaattt
    21601 agacaatact atagatgaac gtgataaacc tttagtcagc ataataacaa gtgtcccttt
    21661 agaatattca tttgataaaa tattacaaaa aataattaga gttaaactca taccaagacc
    21721 agagatattg ctaaaaaatt gtgtccaaga atctaaagca gttggtgcag cagtaacttt
    21781 taaagtcggt gcaaaatgag caatcaagtc tggcataaat tttgcagaaa taggactact
    21841 tataccaaat attagaaata aaataattaa actaaataat ttataatttc ttaaattctc
    21901 tttaaattct ttttttgtaa atgctatgta ttctctcact ttactacctc caaaaataaa
    21961 ttttcaagtg ataattctat tagttccatc tttactggac aaagattcat ttctgcgagc
    22021 gtagatataa ctgttttttg tattgctttt atatctttac catgcaaaac aatttccata
    22081 ttagtttctt ctgaatcttt tagtaaggat ttaataccat cactagactt aaacttttga
    22141 atttcatcat tacttgcaaa ctccagtaaa agtctatcct ttccgtgcat actttttatt
    22201 tctgaaagtg taccactaag ggcaatgctt cctttgttta atatagcaac atggtcacag
    22261 attctttcaa catctgatag tatatgtgtt gaaaatatta cagttgttga gtctttgatt
    22321 ttaagcataa tatctaatat ttcttttcga ccaacagggt caagtgcact tgttggttca
    22381 tcacaaataa gtaatttggg gcgtgaaagc aaagcttgag ctataccaag tcgttgcttc
    22441 attccacgag aaaacccacc tattcgtctc ttttcatttc taagacccac aagtgataac
    22501 aactcttcac tccttatctg tatttctttt tttgaaagtc ccgtaatttc accacaaaga
    22561 gaaagatatt caagtggtct catataattg taaaactctg gaacatcagg cagataacct
    22621 acatgacgat tagaacttgt tttcccatag gtcactttct caccacaaac atctatagtt
    22681 ccactatctg gtttaagtaa cccaagtacc attttcattg ttgtagtctt acctgcacca
    22741 ttcttaccta caaacccaaa gactgaccct tctggtacag taaaatttaa attatctatt
    22801 atctttcgat taccaaaacc ttttgataat ccatttattg agacaatatt catcattctt
    22861 cacctcttcc tattgtaaaa tacaaaattg gtcctattaa actaataaac agtactacaa
    22921 taatccatat tgccttattt ccaattttat aatttggatg tctcaacaca tgaacaagtg
    22981 cagtaattat aagtattaaa tctaaaataa taacaggaac caaaagtggt aagtattcca
    23041 tcaaattttc cataatctta ttcctccaat aaagataatt tttctataag aaatttgtat
    23101 tctttgtttt cacttaatac agaaaacatt ggattatttt taatagcagc aacaatacct
    23161 gcttttacga ttgtatcatc acgtgtaata cctgttccaa tttcagaaat ccatctgtca
    23221 agtttattaa aaaagtcatc cccatgtatt ataattgggt atatatcacg tgttgctaaa
    23281 tctacatatt ttcgtaatga tttaatagct ccttcattat caccatgtat taaattaatt
    23341 tgtgcctgtg ttaaatatga agaaagcata atagcaggag ataatgtatc aagctcaaat
    23401 atatctgaaa ttgctagaat acgtctttct atttccttca tttgtaattt atctgcattt
    23461 tgcaaaatac ccactagatt ttggactaat gatataaggt tctgatatgc accaagttga
    23521 aatgtttctc ttgcttcatc catttgacca ttcatcattt gcccttgtgc aagaagtata
    23581 gattcattta tcatgggaaa attgctgttt tgcaataaat caatcacttg tatgggttga
    23641 tttaacaata tataacaagt tgcctccata ctttttgctt gacggcaaat atctatgtca
    23701 ttacaagttt cctgtatacg aataaatatt tctaatgctt cactaattaa tagttcacgc
    23761 tttttttcat ctacaatatc atagtgatta actattaaaa gacccatccg aaaaatcaaa
    23821 ggaaaacatg aataatattt ttttatgatt tctcttattt catccatgac ttcatcaaat
    23881 ggtttaaccg taaagtcttt gcacagttta ttatacagtt tattaatatc ttccttcatc
    23941 atttgaggtt cataacatat taactcatca acggtaatgt tgaaatatgt cgcgagttgc
    24001 ggtaaaaagg ttatgtcagg atagctttgt ccagtttccc atttagatac agaagcttta
    24061 gaaacgccta tataatttgc aagttcatct tgtgtaattc ctttttcttt acgctttaaa
    24121 atcagcgttt ttgcgatgtt taattcattc atgtcaatcc ctccttatgt ataattatat
    24181 ccttaaacat gtaaagctaa caagcgattt gaagttgact tttgacgaaa aaatcaacca
    24241 cagagaaaca aataaacaag tatataaaat atatatttaa atttatcttt aatctccttt
    24301 gtaaaatatg taaactttgt aaataaaatc catattatat aaaactaata gttaatctat
    24361 ctaaatacag tttttattta tcacgttttt aaatattttg tgtaaaacct cttttaatat
    24421 aaacacctta ttttcatttc tcatttcata ttctttttaa aatactgaat ataactcttc
    24481 gattttccat ttaatccttt tataaagtta ttattcgtat ttattcctct aattataaca
    24541 ggcaactatc atgtattggt atttgtctat tatataaaaa tctataaaag aaaagcagtc
    24601 aatcatttag attaactgct tgtacatata tcttattaaa ctgtataaac tataattttt
    24661 attattctcc attttcatcc caagtcataa atccccatcc actatctgga taaatatcga
    24721 tagacttttc tctaatcata taaccaactt tataacactc ttttgttatt ttattagata
    24781 gtatacatcc ttctccattt atctaattcc atttaattaa cttttctttt caaccttaac
    24841 ttttataaaa tcatcttttt taataatttc atacttattt tctcttcaac atcatcaaat
    24901 gagggtgtct caaaattaac tttttacttc ataaggcact cttttttgta tgaagtaaaa
    24961 tatattttca tcatttcaac aataagtaag aggcttatct ctattttgag acagccccat
    25021 ttgatggtgt tgaacattta aataaggttg aactcgtttt atctctcttt ttaagatatc
    25081 tgtaaatttg cacataaagt ctcttcttct tttacaattc taatagtttt attaaagcca
    25141 aagtatatat ctccatattc tttcgtatat gatttattgg tactctttca tctactccat
    25201 gaccaccagt tttatacgct aagaacttag gtccaaacgc caccatatct ttaaaccaac
    25261 ttgcatatgt gccaccaccc atagcaattg tatatccatt ttcatctgtt acttcttcat
    25321 aggcttctaa taatttttta agtactaaat tactctcatc cacataatgt aagtctttat
    25381 gaacaataac ctctattttc caaccattac aaaaaacctt tttgaatcta tctattatat
    25441 tgtttaaaac tatattttta ccatacctta tatctaactc acatacgatt ttttcctcat
    25501 ttacttttaa tattcctaaa tttaaggtag tgtttttgaa tacctcatca tattgattaa
    25561 tccccaatgc atctccataa taatcactac aaaggtattt tgaaactaat tctatatctt
    25621 ctctatgctg aaaatccata ttatcctcaa ctatctttat taccatttta gatatagcat
    25681 tttctccctt ttcaggatta cttgcatgta cagctactcc tttagtagtt aactctgaaa
    25741 taattccatt aataattttt gcagtacaat gagatggaac aacattttct ttagttcctc
    25801 cacttactat tatgtaaggt ttacttttgt caattttaat ttctttttct atccttatat
    25861 gaacccttcc tttttctcca ttaaccactg gatacatagc atctggagta aatcccatac
    25921 ttggaatttc ttcaccttta tctaagtaat atttgataca tttcatacct gtctcctcat
    25981 ttgtaccaaa aattaatctt attcttttat taattgtagg atacatatcc tctatgtttt
    26041 tcaatacatg tataagcata gatacagcac ccttattatc tatgacacct cttccataaa
    26101 taatattgtc aattactgct cccgaaaatg ggtctacact ccacccctct cctggtggaa
    26161 ctatatctaa atgcattgga atccctatta attcctctcc ctctccaatt tctgcatatc
    26221 caatataatt atcaatattt ttaacactaa aacctaaact agcacagtaa tctaaagttt
    26281 tatctaaaga ttttttaggt cctataccaa aaggacattg tgaaataggt tcaccttgtt
    26341 gtgaatcaat ttttatcaat tcttttagaa attctatggt tctatttaaa ctattatcta
    26401 cacttctgct tatatccata aatcaaccta ctttctatct ttttaaagcc tgtccttaaa
    26461 gaacaggctt ttagcattaa aaattatttt tttgtaaata ctaattcaca aacttcatca
    26521 tcatgagcac atttttgagc aaaatctaat ttaacacctt gtgcacagtc aactcttcca
    26581 taatccccat aacaagctaa tctacaaatt tcttttcttt cttctttagt taatccatat
    26641 tctttccaag cagcatctaa tggacagtga tggaatttta aaacgccatt ctcatctgta
    26701 ttttctacta attccatttc aaacactttt tgtcctttac tgttatataa catttctgcc
    26761 atttttcctg gagtatctgc tacgttatat ttttgacctc tcattttacc aaatccatat
    26821 attgcttctt tacatatatc atctggatta gctcctaatt tttcagcttc tttcataaga
    26881 agatagaacc atactgctct ttctcctgtt gctcctctaa ttccttctaa atattccttt
    26941 tctgtataca ttttttctga catttctttc tcctcctaaa attaaattct atattttaat
    27001 cactgtgaat tagaatattc caatgaatga ccctaccaat gctaatacaa ccaaaattac
    27061 taataactta gttattgtcc acttcttgta tttcattccc caatatacga agaatacaac
    27121 accaagtggt aataaacctg gagcaatcat atttaatgta tcttgaacaa caattgcttt
    27181 accaccaata tcaaatgata gcggagttga aagcttaact gtagttgcag ataatgctcc
    27241 catcataaac attcctagaa ttgaagcacc atcaattatc ttattgacaa taccacctga
    27301 tagaattttc ttaattgaat ctctacctaa agaatatcct aaatgaaata agtaataacc
    27361 ttcgcaaaat agtaatatac taaacattat tgggaaaaat gcacctataa cacttccttc
    27421 tgctccaaat gaagcaccta aagctaagaa tattgtttga aatgtaccaa aatctaaagt
    27481 atctccaatt ccagccaaag gtcccataag accattctta acacctgaaa taacttctcc
    27541 tgggattttt ccttcagtag ctttctcttc ttccatagct aaaactgtac cgtgaattaa
    27601 tcctccccat atagcttgag tattaaaaaa ttgtaagtgt ctctttaacg catctactaa
    27661 atcttctttt ctcttatata acttttctaa agcaggagtg aaacttgcac aaactgctaa
    27721 tgcttgcatt ctttcaaaag agttggataa ttctgctgtc caccaccatc ttagatatgt
    27781 ttttgttaca tctttctttg taagtactct tgtcttatta acttcagttg gaccttgtcc
    27841 atttttaact tctttatttt ttatttcttg cattatactt cacctaccct tttgtcttct
    27901 ttcataaagg taattaaaag agctatacaa acaccaaata ttgcagctgc agtaattgat
    27961 atttcaaaat attgaaccat aaaaaatcct ataataaaca ttggtaagaa tttattttta
    28021 cctatagtaa atattgttgt tgcaaaacct aatgcaggta atataccgcc cattactgtt
    28081 aatccattca ttaaccattg tggtataaca tctaataatg attgaactaa atctgctcca
    28141 aaaaagttac atacaaaaac tattggaaac cttaaaacaa acccaaatat taaagaataa
    28201 agtgttgcac atctccagat gccttttgta ttaccttctt cagcatactt atctgcctta
    28261 tgaaccaata cagcattacc agttcttctt aagttattaa ctaatactcc tataacacct
    28321 aaaggtaccg caatagatac tgcaacttct gcattaacac cagtttgaag tgctactggt
    28381 attgcaataa gagcagctag acatttatct gatggaatat ttccccctgc tgcgaccatt
    28441 cctagataaa ccatttctat acttgcacca acaatcatac cagttgttac atcaccccaa
    28501 agtaatccaa ggctaaaacc tataaataaa ggagacttta gtgttgagaa aagggtatac
    28561 cctgcaagac ctgctgcaaa ccaataccat aatcctgtta caattgccaa catcaaaaca
    28621 gattccattt ataattcctc ctaatttata ttttttattt aaatttcttt aacgcatttt
    28681 catatgtcat agatgattcc tctggtataa cttgtaggac tatttcaacc ccattttcat
    28741 taagttcatt aagttgttct acatcctcat ttcctaatga aacagctgta aacaccattt
    28801 tctttcctgc ttcatttgga atacctccta attgaatctt ttcatattta agtccagctt
    28861 tataagcctt atttgccata tcaacatttt taaatagaat aaatatattt ttatctccta
    28921 aagtgttgtt ttggaatgct tgtcccgcat cttctgctgt taaaataact acttctactc
    28981 ctgatggtgc tgccatttta tatatgtcag ccataaattc atcttgacct aagaagtcat
    29041 caactattat tattttttga gcttgagcga tttttaccca ttttgttata acttgcccat
    29101 gaattaatct gtggtctact cttactaatg ttatttttgc cattatattc acctcataat
    29161 ttatattttt ttaaattctt taattaaatc cacacaaagg ttattatttt ttttgacgat
    29221 tctttctggt aatgtatgta atttatcttc tttgcttaat tcttcatcta cgattaaaat
    29281 tgtctgtaag ctaagtccat ttattgcatc tattcctctt cttatagcaa cagctattgc
    29341 aacacttgat gtagatcctc catataaatc tgatattata attttcacat cttctttatc
    29401 tactacttct tcaacctctt tcataaatac agaaacatct ttttcagaac ttagtgataa
    29461 aacttcatat tgatttgaca atccaaagtt tttatttgtt tcttctacta agacttttcc
    29521 ccaagttcca tggcacagta tgattacctt atatttattc attgctaaat aaactctcct
    29581 ttatagcgat taatattttg gtttaattct acttcttctt catcaggtac catttgaatt
    29641 gatacattaa tattcttttt aataaattca tctattaaaa gaaactcttt tttattaatg
    29701 tatacatttt gagatatttt cttttttcct attcctgtag gtattcttcc aacaataagt
    29761 tttttgatat taatcccttc ttctatcatt ctatttgcag tatctaaatt tttaattaat
    29821 acaaaaacgt tatcatcagc ttgattatta taaaaatatc tccttgattc acttacagtt
    29881 aatattgata aatcaatatt ctttggtgct gccattttta tcaaaccttt agcaataaca
    29941 tcattggttg tttcatcatc tactactact atctttttta cacctagatg cttaacccat
    30001 ttatatataa cttgaccatg tattaatcta tcatcacatc tgcaatattt tactaaagac
    30061 atttttaatc aaactccttt ttctattaca ttgagctaag aactttatta atattgttta
    30121 taccagtttg tcctgcttca atacatgctt ctgctaactc ttctaaactt tttccctctc
    30181 tattaattaa agcttcaagt agcattggaa gatttactcc agtaagtgca tgaaacttgc
    30241 cacctttttt aaggtttaaa cttgcaacat tacttggcga acctcctaat aggtcagcta
    30301 atactaaaac tccgtcacct tcatctaatt catttaactt ttctacaaaa tttttattta
    30361 agtcttcaat attatcattc ctatttagtt taattgctgc tgttctttct gctggtcctg
    30421 caataagttc acagcttttt ataatttctt cactaaaact cccatgagtc ataactatta
    30481 ttcctatcac caatttcacc tcacttttct tgtcactttt tatataagca attgccgtgc
    30541 caacttatgt gtatcaataa caaatggcta ttttacagga tttaatcaaa actttttact
    30601 caattttttt taaaaactta ttatttcgat atgtatcaaa agattttttc aacctgtatc
    30661 aaatctgtat caaaaaaagt aaaaaaaaga tagccttttg gctattttta aattttgtat
    30721 caacttttga atttatctgt atcatattgt gattcaaata tatctaatat atttgcatat
    30781 tccatatctg aaataattat atcgaatgtc tcttctacta gcttaaaact ttcttttata
    30841 actaaatata attctttatt tctactaatt ctttcttcat atgatttata aactaaagac
    30901 tcttttatta ttactctttc aatcatacat gaacaatgaa atataaactt tactaaaagt
    30961 tcatcttgaa ttactaaaga atagtgttca ctaattctat taattacttc ttttaatata
    31021 aaataagcct tttccccatt taaaaaagtc aaagttttac taatattatc aattagtaat
    31081 ctatcaaaat atctaccacc attatctttt tcagcatatg taacttccat atctccataa
    31141 tgtttcttta tagattctat aatattaaaa tataatttct ctaatgtcat ttctggagtt
    31201 agagcctttc tagttgcttc tattacaata ggtgtactta ccatatctat tgcttttact
    31261 tcttttcctg tagcatcacg aataacttta gcaaatgtta aaatagaccc catatcagtt
    31321 aaaattaaaa ttccctttcc tttatcaatt ttattggcta tttctacagc ttgtactaaa
    31381 atatcctgaa ctctatcttc taatcccata ttaattgcca ctgcatggtt acaatccagt
    31441 aaagtattgg ctacattcac catgctagat gctgtagatt caccatgcat tatgactaat
    31501 attgctatac tctcctcatt taatcccaca tacgataaat aaagaaatgt tgctaaaaaa
    31561 attgcttcat cttctggaaa ctctactcca tacattaatg acatgttaga taatatttct
    31621 tttgctatgt tatattcata tgctctttcc ttcataattt catctttatt aggatgcttt
    31681 actatatttc caatctttat tctttcaatt aaattctgaa tatgtaaagc tatagctttt
    31741 attaatttat ctaatccttc tcttttgctc catttatcat gcttttgtaa aatatatttt
    31801 acagtagttg caattgaatc attaacaatg ttgttataag ctgtttgatt actactatta
    31861 tatataaaag tattcattaa attataggaa tacttctgta tgtctccatc tataacactt
    31921 ctaatttgtg actctttaat accttcttgt tgaagtttat tccaagtttc ttttatcatt
    31981 ccataaaaat ctaaatcata ttttttattt tctattaata aattttcact ttcaaaatcc
    32041 ttttcttctg gtaaaaaaat aatatcatct tttccaataa aactgaaatt ttgtacaaac
    32101 tcctcttttc tattatttcc atataatgat tctctaatat tatttggtag aagagagagc
    32161 cttacatata cacattccct tttataggtc atataatcta aaaaagctct tgcacaaatt
    32221 aattgtatat cgctttttaa ttggcctatg tttcccttac acttatataa aataaatgcc
    32281 ttaataactt cttttgaaac ctttaactta acaccaatcc taacactctc ttctttaaaa
    32341 aaataacata taagcttcat tctctcttga aagcctcttt cctctaaaga cggaatcttt
    32401 atagttacag gtattcttct taaaaacgtt tccaacattg ctgtttctgg gtcttctgta
    32461 gtggctgcaa ttatcataac tctacactcg tgtaccttat tagcctctcc taaccttcta
    32521 tatatccctt tatccataag cagaaatagc atttcttgtc cctctgcaga taatctatga
    32581 acttcatcta aaaacaatac tccattatta gcttcatgta caataccata cttatcttta
    32641 tcagctccag taaatgctcc ttttacatat ccaaaaagct gcgctaataa taattgctga
    32701 ttatcagaat aatcagcaca gttaaatatg ataaatggtg cattatcact atcttctttt
    32761 atagttaaac tatacttata catatattct gcaaatatag atttaccaac tcctgtaggt
    32821 cctattatta atgtactcaa ccccttaggt ggatatatta ctgctgcttt agcctgttct
    32881 atttgggacc ttaaacttcc atcacatcct actaaattaa aaatattgtt ctcttttttt
    32941 atactctcta aaatatactc atctttgata gaatcatttt cactatattc gtttatagag
    33001 ttaatattat caaatatccc tttatttagt ttctttccta aaatactctc aatactttct
    33061 ttatataagt actgaacagg cttacccttt atctttataa taatactttc ttttattaat
    33121 tgatttaatt ccttactagc attacttcta tttataccta cttcagtagc tatatattct
    33181 gcatcaaatc caaatctaat gccattatta aagttcaagt gttcttctgt taaccttttc
    33241 aagcaaatta aaactttttc tttccttgat agcttctttt gtgtcatagt taacctcccc
    33301 tataagcagt aatactgtta aacatttatt attaagctta attctacaat attttaatat
    33361 ttttgtctaa ttatgtaact attgattttc tttcctattc gttttataat ataaattacg
    33421 ttaccctcat cttttataga ggaggaattt atatgaattt tttatcagat ttacacaccc
    33481 actctatagt cagtggtcat ggctatagca ctttactaga aaatattaat tattgtaaag
    33541 aaaatggaat taagatacta ggtacatctg aacatggtcc taagatgcct ggttctcccc
    33601 acagatggta ctttcacaac attcaaaata tcccaagaat cataaataac attataattt
    33661 taagaggctg tgaagcaaat atactagata ttaaaggaaa catagaccta gagccttttg
    33721 taattcctcg cttagattat cttattttgt catttcacga agctgtgttt tctccaaata
    33781 cattagagaa taatactaaa gctttaataa atgcaataaa taagcatgat aatattgaaa
    33841 tattaggaca ccttggaaac ccaaactacc caatcgacta tgaattaatc attaaattag
    33901 ctatagaaaa gaatatctta atagaaataa ataattgttc tattaaaggc gtgtcaagaa
    33961 atggtagctc ggataactgt aagtatattg ctacactttg caaaaaatat ggtgctaaaa
    34021 tcatactgac ttcagatgct cacatttgtt ttgacatagg taactatgag tattctgaaa
    34081 atatcttaaa ggaaattaat tttccagatg aattaataat gaattatcct aaaaaactta
    34141 ttaatcattt tcaccaaaaa ggtaaattac tagatgttga ttatacaaat atctaagtaa
    34201 taatacatgc ttactttaac tcccataaaa attttaatac tatttatatt atatgaaata
    34261 aagaaattat aataaagaag ctatttaata aagcttaatt acttttatga acttgatgga
    34321 ttgatatggt ctatgatagc aatagaaatt ataacatttt taataaactt agttttatat
    34381 accttatagt ataaatctaa aaaagcatca tctaaaaagg agctgtcaca tgagcaaatt
    34441 aaatttgttc tatgatagct ctttttttac ttattttaat taggtatatt tttaattatt
    34501 ttatataaag cttatgttct aagtaaaatt atgcataaca aacaaaggta taaaaatacc
    34561 attttttcag ataatattta attataaata ataaatttag aatacttctt ttaaatgatg
    34621 agagtctaga aatttttatg tgtattcttt aaataaaaat ttccagactc tagccccttt
    34681 ttgaatattt taaaatttaa cttaaactaa gcaaattcta taatagctta tctctaaa
//
LOCUS       DAMJVI010000033        33902 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.035, whole genome shotgun sequence.
ACCESSION   DAMJVI010000033 DAMJVI010000000
VERSION     DAMJVI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33902
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.035"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            42..506
                     /gene="bcp"
                     /locus_tag="PC545_001680"
     CDS             42..506
                     /gene="bcp"
                     /locus_tag="PC545_001680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDF2456451.1"
                     /translation="MLSIGTKAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDIQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEKQE"
     gene            516..1259
                     /gene="yaaA"
                     /locus_tag="PC545_001681"
     CDS             516..1259
                     /gene="yaaA"
                     /locus_tag="PC545_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDF2456452.1"
                     /translation="MIIMLSPAKNMKNIEVFDRDLSLPCFIDNTKEIVENIKTFGIED
                     FKNKMKINEKLAVLNKNRFESIKFDRLGNPAILTYDGIQYKNIEAENFTRKDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQTKLQVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGILKVESTQAKKARGMMTKYIVKNRIRD
                     IEELKKFDLEGYKYKENLSNNSEYIFVKE"
     gene            1387..4044
                     /locus_tag="PC545_001682"
     CDS             1387..4044
                     /locus_tag="PC545_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDF2456453.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPPRTTLQMLWSQITDAMVMILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKECVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     DTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMAKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEALEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITEVDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGMVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTIAYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSAFLIAVILFVPVFRNAFNLTVLNNL
                     EWLITIVLAIMSVIQVEVGKIIKRKKLKK"
     regulatory      4098..4200
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4310..5575
                     /locus_tag="PC545_001683"
     CDS             4310..5575
                     /locus_tag="PC545_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HDF2456454.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVSSVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENALKIARFLEKHENVDWINYPGLEDNKYYENAKKYLSRG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            5605..6495
                     /gene="metA"
                     /locus_tag="PC545_001684"
     CDS             5605..6495
                     /gene="metA"
                     /locus_tag="PC545_001684"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDF2456455.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKELKYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTGFNVSVEFIKISTRESKRSCNEYVKEFYKTFDEAKGE
                     YFDGFIITGAPVEQMEFEEVDYWNELEEIMDYSKRKTKSTLYICWAAQASLYKYYNVK
                     KLPLSQKCFGVFKHKVDKDSKIVDGFENEFFAPHSRHTTVNIEALKENKELSIVSHSK
                     EAGPYIITNSRDVFVMGHSEYDKYTLDKEYKRDINKGDKISIPQNYYINDDPSEEPTV
                     KWKKHSELLFRNWIKNYLIQ"
     gene            6986..7429
                     /locus_tag="PC545_001685"
     CDS             6986..7429
                     /locus_tag="PC545_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2456456.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLSDFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQEHISSSIFDSYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            7460..9277
                     /gene="ftsH"
                     /locus_tag="PC545_001686"
     CDS             7460..9277
                     /gene="ftsH"
                     /locus_tag="PC545_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDF2456457.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLSILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALTEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKACH
                     KNAIEILENNIDKLHELANYLLEKETITGDEFMEILNLN"
     gene            9455..12157
                     /locus_tag="PC545_001687"
     CDS             9455..12157
                     /locus_tag="PC545_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HDF2456458.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYAAGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKRFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     SSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETAAKREENTKMIYQIIKGFLRLSNKDDIVNKGIELLSLSLSRDVICYLNVDKHNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCIDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVENNKDKGSTFKFSLPKENE"
     gene            12191..12889
                     /locus_tag="PC545_001688"
     CDS             12191..12889
                     /locus_tag="PC545_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456459.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEAQQKDDVKSTFEVDKLLIDFDKRKVIVDDVEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            13246..14202
                     /locus_tag="PC545_001689"
     CDS             13246..14202
                     /locus_tag="PC545_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HDF2456460.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYNFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            14379..14534
                     /locus_tag="PC545_001690"
     CDS             14379..14534
                     /locus_tag="PC545_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456461.1"
                     /translation="MKGILKELTAKLYSFFYFMFWGFYFSAGYFFYKNICTSFLVRPY
                     FSDFEHK"
     gene            14664..15677
                     /gene="aroF"
                     /locus_tag="PC545_001691"
     CDS             14664..15677
                     /gene="aroF"
                     /locus_tag="PC545_001691"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDF2456462.1"
                     /translation="MIVVLKMGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKVVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEDQIVSIAKDVKKSGAGFLRGGAFKPRTSPYAFQGLKYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            15699..16763
                     /locus_tag="PC545_001692"
     CDS             15699..16763
                     /locus_tag="PC545_001692"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDF2456463.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILKNIGEEIKKIYFGNKIFIVTD
                     ENIAKLYLDKVKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGISFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKYYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVDAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            16763..18076
                     /gene="aroA"
                     /locus_tag="PC545_001693"
     CDS             16763..18076
                     /gene="aroA"
                     /locus_tag="PC545_001693"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDF2456464.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSMAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFDRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTIKDFGVEI
                     INNNYKEFIIKGNQTYKARDYKVEGDYSQGAFYLSADAIGEDISILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNGTLIDASQCPDIIPVLSVVASLSIGRTTIIN
                     AGRLRIKECDRLHAINVELNKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFKMLGGRIDEWNMGK"
     gene            18054..19133
                     /gene="aroC"
                     /locus_tag="PC545_001694"
     CDS             18054..19133
                     /gene="aroC"
                     /locus_tag="PC545_001694"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDF2456465.1"
                     /translation="MSGIWGNNLKVSIFGESHGNAIGINIDGLPSGIELDLDNIDKEM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEIGAHIKKIK
                     NIEDMSFDYVNISKQQLSNLQTLELPLLDLSKEEAMKNTIIDAKNQGDSVGGIIECAV
                     VGINVGLGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKDKKDDILYIKGRHDP
                     CIVQRAIPVIEAVTAIGIFDLMKGR"
     gene            19136..20332
                     /gene="pheA"
                     /locus_tag="PC545_001695"
     CDS             19136..20332
                     /gene="pheA"
                     /locus_tag="PC545_001695"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDF2456466.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETVQFFNSIMEISRGLQKRFIDNDGEQKIEFSLDS
                     SNKNFDLSNVNKYKSLKDELNKKNILVGFPGKSGSFTEEALNKFFNKKISKKQFKEFE
                     DVFIALKNKEINYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLEDIKEIYSHPQGIGQCSEFLKQNSTWKLIPFNNTATSAELVRKLQDKTKAAIASKK
                     AASIYGLEIISPCINDIANNYTRFVVISNQIHIEEESNKMSVVFSVEHEAGKLYKVLG
                     YFAKNNINMTKIESRPMKNASWRYFFYIDFECSIYNAKVYDLLELIEHNTAYFKFMGV
                     YKNKTE"
     gene            20379..21188
                     /gene="aroE"
                     /locus_tag="PC545_001696"
     CDS             20379..21188
                     /gene="aroE"
                     /locus_tag="PC545_001696"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2456467.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDIS
                     KLDGAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLRENMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAALTYFIDSGIEKLYVSTRKKDDKKLLNS
                     KAILIDYEELKHIKGDIILNATPVGMYPNVGISPVSKSIIQNFDILIDLIYNPGETEF
                     LRIGNSMGKKTCDGLYMLVGQAIKSQEIWQDTKIDNSILDVIYNELKLEFL"
     gene            21200..21733
                     /locus_tag="PC545_001697"
     CDS             21200..21733
                     /locus_tag="PC545_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDF2456468.1"
                     /translation="MINKTKEKLILIGMPGSGKTTIGKLLAKEYNCSFCDMDDYIIQI
                     SQKSIAELFSEGEDIFRNYETQACRELSISDKTVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNNAVY
                     NITNAVSENFKFDFKEK"
     gene            21754..22593
                     /locus_tag="PC545_001698"
     CDS             21754..22593
                     /locus_tag="PC545_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDF2456469.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNKNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKKGIDFASEQVFNGANYIITPTGRNNIKNLELVENLI
                     LEIGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEGRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLVKEAIFNNDKNKLIEYFKKSSIRREA
                     LEK"
     gene            22861..24342
                     /locus_tag="PC545_001699"
     CDS             22861..24342
                     /locus_tag="PC545_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2456470.1"
                     /translation="MKKNRLIAKNISIAFIVLFFFSVFTFFYVGNINRVLEYETNDII
                     TVTIAGWIILSFLFLGIIIYILYSKANSQKTIEKVAYTDFVTGYSNWRKFELDVTNLL
                     KKTSQNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELTDINETFARVSAD
                     NFNILLTYNKKEDIINIIKKIMANNELVNLSFGIYEIKDKDLSVSVYSDRASLAKSSI
                     KNNSDVNFAFFNDKLREKLLFEDKIEKEMEYALESGQFVMYLQPKYNIKLDKFCGSEA
                     LVRWQYTEKEVIYPGDFIPIFEKNGFIRKIDMYILEQACKEIRSLFDKGISPLPISVN
                     FSRVDFFKKDFIENIVNICDRYKIPYSLIEIEITESSMFGDTDTLFNVSRNLQDIGFI
                     VAMDDFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRV
                     VAEGIETRSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            24375..27812
                     /locus_tag="PC545_001700"
     CDS             24375..27812
                     /locus_tag="PC545_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2456471.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSVANMVSYFNTIDNSKIVPLLANISKESDFQRISIV
                     SPDGISHTSDGKIINVSKYQYFKKAIKGESNISEVVKSPIDGKDTFVIATPVFNKNNK
                     IIGVLYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYEDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIMLIIYIIYKNNKSQKDILKANKEISSITNSIPGG
                     VQKCTLGETCNINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEIFKQL
                     YKNKTVELEYRIITKNGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENVLGGIIITEFNDDFTIKYANSGYLSMIGYTREQLENELKYKACNLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIVDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITKNV
                     PYSLVESGVIHKDFSDDFINMYENVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNESW
                     QKPFGIKPDNNYSQMVKTIANSIVHKDNKETFLNTFLCENLLDAYKNNVTEIKLEYQR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEEASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKNLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENIKKKAEKICDIFRNSYTENKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKLADK
                     CEKSKPVLKEILDSIDNYIYIVNPNTYELLFVNNKVSRLDLEIKLGETCYKSLLKSDR
                     PCNNCPLNGLEENKHSKFKIETNTFGKNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            27830..28576
                     /locus_tag="PC545_001701"
     CDS             27830..28576
                     /locus_tag="PC545_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDF2456472.1"
                     /translation="MLNSITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIVLIIYQ
                     IFKKKIKEKKVFYRVLKIIISIFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TTPSTTLKGRLDTALKYLKINDNCYVVVSGGKGSGEKITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKLKIEEHSQRKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIIFDR"
     gene            complement(28525..28833)
                     /locus_tag="PC545_001702"
     CDS             complement(28525..28833)
                     /locus_tag="PC545_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2456473.1"
                     /translation="MNFLKENSTFYFATVEDNKPRVRPFGFIMDYEGKLYFGIEKYKT
                     SYKQLLVNPNIEISTTSKVGKWIRIKGIEKTRYEFISSLFIYYLSKMIVFHNAKNSLV
                     "
     gene            29636..33769
                     /locus_tag="PC545_001703"
     CDS             29636..33769
                     /locus_tag="PC545_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HDF2456474.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANIPVVLQDIATGVRLVVL
                     TDASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFATAIVGPVPIAEMPPISPPIGST
                     NLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIENILDSCVSVSNINLITDADNGTF
                     GFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSNVIGA
                     WWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNYLFSTWILNLFKVTGYPP
                     PQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEVGTVINSQNNNQLTVEFFSEGES
                     AIGNDYAIDDVSLREIAVPTFTPIKTSNTETANVGDIITYTVTLDNTCDSPLTNVFFR
                     DLIPNGLEFIPGTVTVDGVSVPGVDPNTGFSLPDIGGGTGVEVTFDVVVESIPNPNPT
                     NNIANIDYSYTPVEGGIPNDFSVDSNPVPVEVISADIEVTKLSEPTIVNPGEELIYTI
                     KVVNNGPFPSENVVLTDDVPASIINPEYSLDGGVTFQPWTGSLNIGTLEVGETRVIII
                     RGIVNPSTVGIITNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNSAVPG
                     TLLRYTIQVENLGPANAENVILNDDIPASIINPEYSLDGGASFQPWNGSLNIGTLNSG
                     ISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISIIKVAD
                     EDVAVPGEEFVYTIEIFNEGPSNATNVVLTDDIPDVILNPEYSLDGGATFQPWNGSLN
                     IGTVAPGQLIRIIIKGLVSSTATGDITNTAEVSADVPEPVTDSSTVTTPIVPSADIEV
                     IKTSNMDTAVPEETFSYTITVTNLGPSAAQSIVLTDDIPDVILNPEYSLDGGVTFQPW
                     NGNLSIGTLDAGEIRSIIIRGTVSQTAVGTIINTATISSPTPDPNPDNNTSTDETDIS
                     PLADISVIKGNEPVAIPGGRFIYGIEIANAGPSFAENVTLTDNIPASILNPEYSIDNG
                     VTFQPWNGSLNIGTLDAGEIRNIIIRGTVSQTAIGTIINTATVSSTTPDPNLNNNTST
                     SEAEVSSSADISVVKRSNQTVVVPGDVLDYTIEVRNAGPSTAQNVTLTDNIPASILSP
                     EYSIDNGVTFQPWNGSLSIGTLDAGEIRNIIIRGIVSQTAIGTIINTATVSSTTPDPN
                     PDNNTSTSEIDISSSADISVIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTLIDNV
                     SDKLKKAVFSLDRGVSFQPWTGSLNIGTLPAGTLRVIILRGIIKSTCLDRLTNIAEVT
                     STTPDSNLNNNISRVQVEIKQFCCNDCGCKDCCCNCCCNDCGCKNCCCNCCKNDFWC"
BASE COUNT        13618 a         3771 c         5846 g        10667 t
ORIGIN      
        1 atagtataaa attattaata aaaacaaaag gagagatatt tatgttgagt ataggaacaa
       61 aagcaccaga atttacacta gaagataaag atggaaataa ggtaagtatg tcagatttta
      121 aaggcaaaaa agtagtagta tatttttatc ctaaagataa tacaccagga tgtacacgtc
      181 aagcttgtgc ttttagaaat gcatatgatg gatttaaaaa ggaagatata caagttattg
      241 ggataagtaa agatagtata aaatcacatc aaaaatttgc agaaaagcat gaacttcctt
      301 ttattttact ttctgaccct gacttagttg ctataaaagc ttttgatgta tggaaagaga
      361 aaaaaatgta tggaaaaact gcattgggag ttgtacgtgc gacatatatt atagatgaaa
      421 atggaattat tgaaaaagtc tttgaaaaag caaaaccaga cacaaatgca caagagattc
      481 ttgaatattt ggaaaaacag gagtaataag aatttatgat tataatgtta tcaccagcta
      541 aaaatatgaa gaatatagaa gtatttgata gagatttgag tttgccttgc tttatagata
      601 acacaaaaga aatagtggaa aatataaaga catttggtat agaagatttt aaaaataaaa
      661 tgaaaattaa tgaaaaatta gctgtattaa ataaaaatag atttgaaagc attaaatttg
      721 atagattagg aaaccctgca atcttaactt atgatggaat acaatataaa aatatagaag
      781 cagaaaattt tacaaggaaa gatgaagagt ttgcaaatag ctgtattcga atcataagtg
      841 gattgtatgg tgttgtaaaa ccatatgata gtatatatga atatagatta gaaatgcaga
      901 caaaattaca agtaggtgag tttaagaatt tatacgaata ttggggaaat cgtatatata
      961 aagaactaat caaagaaaaa acagctatcg ttaatctttc atcaaatgaa tacagcaaat
     1021 ccatagaaaa atttattaaa gatagtgata catatattac atgtactttt aaagtaaata
     1081 aaaatggcat attgaaagtg gaatctacac aagcaaagaa agctagaggt atgatgacaa
     1141 aatacattgt aaaaaatagg ataagagata ttgaagaact gaaaaagttt gatttagaag
     1201 gttataagta taaagaaaac ttatctaaca atagtgaata catatttgta aaagagtaga
     1261 agttttgtta aatattaaat ggcattgtta aatttaggta aaatatactt agtgtgtatt
     1321 gaattgaact tgattttaaa atataattat tcttaaatta aaaaaagaaa ggagtggtct
     1381 tccaaaatgg aaaatcacaa acagatgcct tggcattcaa gagcaacgca tgaaatttta
     1441 gaaagtctag attcatcaga ggaggggctc accgataccg aggcggaaaa acggcttaaa
     1501 gagaatggct taaatgaact tagaaaaaaa ccacctagaa caactttaca aatgctttgg
     1561 agtcaaatta cagatgctat ggttatgata ctaataggag cagcaatatt atctttgata
     1621 tttggcgaat ttacagaagc ttttgtaata ctaataattg taacagtaaa tgcagttatt
     1681 ggaattgttc aagagaaaaa agcagaatca tcattagaag cattaaaaaa tatgaattca
     1741 cctacagctc gtgttatgcg tgatggtgaa gaaaatgtta ttccagcaag taatcttgta
     1801 gtaggagata ttgttttttt agaagatgga gctatggttc cagcagacct tcgtttaata
     1861 gaaacatcaa gtttaaaaat tcaagaggct tctttaacag gagaatctgt tccatcagaa
     1921 aaagatgcag atacaatctt acctaaagaa tgtgtattag gagaccgtag caatatggcg
     1981 tatacatctt caatagtcac atatggtaga ggtgtaggtg ttgtagtagc aactggtatg
     2041 gatacagaag tgggtaacat agcacatctt ttagattcac aagatgattt tgatactcct
     2101 ataaaacgta aacttaatac tgttggaaag acattaagtg tcataggtat tattgtatgc
     2161 gttgttatat ttgcaatagg tgcattttat cagagacctc tcttgccaat gtttatgatt
     2221 gcaatttccc ttgccatatc aattattcca gagggattac ctgcaacagc tactattgta
     2281 atggctttag gcgtacagcg tatggctaaa agaaatgctt taattagaaa attacctgct
     2341 gttgaaacat taggaggagc tacagtaata tgttgtgata aaactggtac attaacctta
     2401 aataaaatga ctgttacaca tattgctgtc aatggagatt ttgaatctgg aaaagtgaca
     2461 tcaatagata ctgcatctga aaaacatcca ttagtgtata aagagattgt ttatgctggt
     2521 gcactttgta atgatgcatg tattaatcca gataaaacag atgaaatatt aggtgaccca
     2581 acagagggcg cattaatttt tatggccaaa aaatttggaa tagatcaaga agctcttgaa
     2641 gaagaatatc cacgtgtatt tgaacagcct tttgattcag atagaaaatg tatgacaact
     2701 gttcataata tagatgaaaa gattattgct tatactaaag gagcagtaga cgaaattctt
     2761 gacctatgta ctaaaatatt aacttcaaaa ggtgaaagaa acattaccga agtggataaa
     2821 aagaatatac acgaattatg tcttaacatg tctaaagatg cattgcgtgt acttggtttt
     2881 gctaagcgtg agatttcatc tataccaaaa gaagatagtg aaaatataga atatgattta
     2941 acatttatag gtatggttgg tatgattgac ccaccaagaa ctgaggtaat tgatgcagta
     3001 agtacttgta aagaagcagg tataagaacc ataatgataa caggtgacca taaagttact
     3061 gccacaacaa tcgctcatga gttaggaata tggagtgaag aaaatactgt gatatctggt
     3121 gatgaacttg ataatttgag tgatgatgaa ctagatgaag ctgttaaaaa tactacagta
     3181 tttgctcgtg tatccccatt tgataaactt agaattatac aatctctaaa aagaattgga
     3241 gaagtaccag caatgactgg ggatggtgta aatgactctc ctgccttaaa gtctgctgat
     3301 attggtatag ccatgggtat aagtggtaca gatgttgcaa aagattcttc tgacatgatt
     3361 ttaatggacg atagttttac aacaattgct tatgcaataa aagaaggtag acgtgtatat
     3421 agaaacatac aaaaagttat acaatttttg cttgttggta atatagcaga gatacttacg
     3481 ttgtttgtag caatattatt aaattgggat actccgcttc ttgctgttca tattctatgg
     3541 gtaaaccttg caactgctac gcttccagcc cttgctcttg gagttgaccc accaagcaga
     3601 aatataatga agcaccagcc agtgaaatca accactttgt ttgaaaaaaa tcttatacaa
     3661 agagttgtta cccaaggtat atttgtggca ataatgacta ttttagcata taaaattggt
     3721 cttaatatag aaaatcattc tgtaggtcaa acaatggcat tttgtgtatt agcattttca
     3781 cagatgttac gagcatttag tcagcgttca aatacagatt caatgtttaa tagaaataat
     3841 ggtaaaaatc catttctttt aatctcattt ttaacttctg catttttaat agcagtaata
     3901 ctatttgtac ctgtttttag aaatgctttt aacttaacag ttttaaataa cttagagtgg
     3961 cttataacga ttgttttagc cataatgtct gtaattcaag tagaggttgg gaaaattatt
     4021 aaaagaaaaa aattaaaaaa atagttgaca aaattaggaa aaatatataa gatataaaac
     4081 atagaaaaat taaataaatc ttatccagag aggtggaggg actggcccta tgaaacccgg
     4141 caaccagtat atttatatac attggtgcta aatcctgcgg cgaaagctga gagatgagta
     4201 taattaagct tatacagagc ctgagttata tactcaggct ttatttattt taactctcat
     4261 ctggtaaata gtttaatttt ttttactaaa atctaagggg gatacaagaa tgtataataa
     4321 agaaacaata tgtgtgcaag gaaattataa accaggtaat ggagaaccaa gagtactacc
     4381 tttatatcaa agtacaactt ttaaatatag cagtatagac caacttgctg aattatttga
     4441 tttaaaagtt gatggacata tatattcaag aataagcaat cctactattc aagcttttga
     4501 agaaaaaata agtttactag agggtggagt atcttctgta gctgtatcat cagggcaatc
     4561 tgcaaatatg ttggcagttt taaatatatg taaatcagga gatagtatac tttgttcttc
     4621 aaaagtatat ggaggaacat tcaatttact aggacctagt cttaaaaaat ttggtataga
     4681 tttaatatcg tttgacttag attcaagtga agatgagata gtagaacttg caaaggaaaa
     4741 tactaaggtt gtgtttgcag aaacacttgc aaatccaact cttgaagtca tagattttga
     4801 aaaaatagca aatgtagcta agagaattaa tgttccattt attgttgata attcattagc
     4861 atctccagtg ctttgtaacc ctttaaagta tggagcaaat atagttactc attctaccac
     4921 aaaatattta gatgggcatg cttcaagtgt tggaggaatt atagtggatg gtggaaactt
     4981 taactgggat aatggaaaat ttccagaatt agttgagcca gacccaacat atcatggtat
     5041 aagctatact caaaaatttg gaaatgccgc atatgcaact aaagcaagag ttcagttgct
     5101 tagagactat ggaaattgtt taagcccatt caatgcatat cttactaatt taaatgttga
     5161 aacactacat cttagaatgg agagacatag tgaaaatgca cttaaaatag ctagattttt
     5221 agaaaaacat gaaaatgtag attggattaa ttacccagga cttgaagata acaagtatta
     5281 tgagaatgcc aaaaagtatt tatcaagagg atgtagtggt gttttatcat ttggagtaag
     5341 aggtgggtta gaaaatgcca aaaaatttgt ggaaaaatta cagatagcat ctttggttac
     5401 acatgtttca gatgtaagaa cttgtgttat acatccagct tcaactactc atagacaatt
     5461 aacagaagaa caattaattg catctggagt attgccttca ctaataagat tatctgttgg
     5521 aatagaaaat gtagaggatt taatagctga tttaaatcaa gctttaaatt tctaatattc
     5581 taagagaaag agaagtgaaa agatatggct ttaatactac caaagggatt acctgttatt
     5641 aataaattat tagatgaagg aatagatgta atttataaag aagattttaa aaaggaatta
     5701 aaatatgaag aaaacataga cactaagata gctatactaa atctaatgcc tataaagata
     5761 gatacagaat tagatttact aagaaggata gataaaacag gattcaacgt gtctgtggaa
     5821 tttattaaaa tttcaacaag agaaagtaaa agaagttgta atgaatatgt taaagaattt
     5881 tataaaacat ttgatgaagc caaaggagag tattttgatg gctttataat aacaggagct
     5941 ccagttgaac aaatggagtt tgaagaagtt gattattgga atgaactaga agaaattatg
     6001 gattattcta agagaaaaac taaatctaca ttatatatat gttgggcagc tcaagcaagt
     6061 ctttacaaat actacaatgt aaaaaaactt ccactaagtc aaaaatgttt tggagtattt
     6121 aagcataagg tagataaaga tagtaaaata gtagatggat ttgaaaatga attttttgct
     6181 ccacattcaa gacatactac tgtaaatatt gaagctttga aagagaataa agagttgagc
     6241 attgtgagtc attctaaaga agctggtccc tatataatta caaattcaag agatgtgttc
     6301 gtaatgggtc atagcgagta tgacaaatac actcttgata aagaatataa gagggatata
     6361 aataaaggag ataaaatatc tatacctcaa aattactata ttaatgatga cccttcagaa
     6421 gagccaactg taaaatggaa aaaacattca gaattgttgt ttagaaattg gataaaaaac
     6481 tatttgatac aataaatttt aagagtatat aaatgttaac taaaaaaata tagaaattta
     6541 taggtataaa gttaatctta atgatacttt ataccttatt tttttaaaat ttgaccttat
     6601 aatagtagtt ttatatttta agaaaatcac tttaggtgta tgtagataga ggtggcttta
     6661 attttatata tggtgcgata atgggagact aaacctttat agtatcaata aatttgtatt
     6721 ttaactttta aaagagatta aaaagggaga aatcagtgta aaatatatat atagattttt
     6781 tattaaattt tattaaaaat atcctgtata aaattaaata tcaataagat aacaaaattt
     6841 caattgaagt tacaatattg taattgacaa taaatactta atactataat atttaataca
     6901 ttaaacatta tagtgttaag tatttattta cagtatgcac ttcagcctaa atttgaaata
     6961 aagtattttc gtagggagcg tttgtatgga tgagatgagt tttaagaaag agctactaga
     7021 gttgactaga gatataaata tgaaatttac aactttatta agtgattttt atcagccact
     7081 tggtattaca gcagtgcaag ctttgatttt atctgagtta tgtgaacatg gagaaaaaaa
     7141 aataagcgat ttagggaaga acttaaacat gacaaatagt aatgtatcag taatctgtca
     7201 aaggcttgaa aaaaatggct ttttaaatcg cataagagat atagaagacc aacgaattgt
     7261 aaaagtaaaa gttacaaata aatcattgga tatacaagaa catatttcaa gtagtatttt
     7321 tgatagttat tttgaaaata tgaccagtga aaaattgcaa gatatggaag atataataga
     7381 aggtttagag aaattaaata aacttttaac ttacattgat tgcaaataac ataaaaataa
     7441 tttaaaggag aataaaatta tggatgaaca taaaccccaa aaaaggtcag cactctatta
     7501 ttatacagtt ataataatag tagtaatgtt tttaaatgct ttaatatttc caagtttaat
     7561 ggataaaaaa atggttgaga ttgactatgg aaagtttctc acaatgttgg atacaggaaa
     7621 ggttaaatct gttgagatag aaaataatag aatagcaata aatccatcta gtagcaagga
     7681 taagaatata tacataactg gtagaatgga tgacccagaa ttggtagata gactaaaaaa
     7741 tgcaaaagta gaatttacta aggttattcc taaggaaaat tcacctcttt taagtatttt
     7801 acttacatgg ataataccta taggtattct tatgctattt ggtaacttaa tgatgaagtc
     7861 tatgcaaaag cgaatgggtg gtaatgctat gcaatttgga aaaagcaacg ccaaaatgta
     7921 tgtatctgct caaagtggaa aaagatttaa tgatgtagct ggtcaagatg aggcaaaaga
     7981 agctttaact gaaatagtag atttcttaca caatccagaa aagtataaaa agataggagc
     8041 acaaatgcct aaaggagcac ttttagtagg acctccaggt acaggaaaaa cactccttgc
     8101 aaaagctgta gcaggtgaag caaatgtacc atttttctca atatctggtt ctgagtttgt
     8161 tgagatgttt gtaggaatgg gagcttctag gattcgtgat ttatttaaac aagcaaaaga
     8221 aaaagctcca tgtatagtgt ttatagatga gattgatact attggtaaaa agcgtgataa
     8281 tggaagtgga ttaggtggaa atgatgaacg tgaacaaact ttaaatcagt tgttaagtga
     8341 gatggatggt tttgatggtg gaattggagt agtaatactt gctgctacaa atagacctga
     8401 cagtcttgac aaagcattac ttcgtccagg aagatttgat agacgtatac cagttgaact
     8461 tccagacctt ggaggacgtg aagctatatt aaaggtacat gctcaaaatg taaatgtaag
     8521 ctcagatata gattataatg caattgcaag gtctacatca ggagcatcag gtgcagaact
     8581 tgcaaatatt gtaaacgaag cagcactttt agcagttaag tctggcagag atatagtaga
     8641 acaacaggat tttgatgaat ctgttgaaac tgtaattgct gggtatcaac gtaaaggagc
     8701 tgtaatttca gataaggaaa agaaaattat agcatatcat gaaataggtc atgcacttgt
     8761 tgctgctatg caaaaacatt ctgcacctgt ccataagatt actataatac ctagaacttc
     8821 aggagcattg ggatatacta tgcaagttgc tgaagatgag agtgtactta tgtctaaaga
     8881 agaagcaatt gataaaataa ctacttttac aggtggtcgt gctgctgaag aggttatctt
     8941 taacacttgt acatcaggag catcaaatga tatagaacaa gctactaaaa ttgcaagagc
     9001 tatggttact cgccttggta tgagtgaaga gtttgatatg gtcgctcttg aaactgtaag
     9061 caatcaatat ttaggaggag atacttctct tgcttgttct tccgacactg ctgctaaaat
     9121 agatgaagag gtatttaaaa tagtaaaggc ttgtcacaaa aatgcaattg aaatcttaga
     9181 aaataatatt gataaattgc atgaacttgc taattattta ttagaaaaag aaactattac
     9241 aggtgatgag tttatggaaa tacttaactt aaactaaaat gattatacac aagataaaaa
     9301 tgcctattgt taggcatttt tattttacaa ctaaattttt taaattcata tttaaatctt
     9361 tacataatct taatcattaa aaatacatct taactacaat tttatatcgg aattgatatt
     9421 ataatagtaa tgaatggtgt aaggaggttt tttaatggat agacctaatc ctgatattct
     9481 attagaaaaa attaaaaatg aagaagaaaa actaagtaga ggtcaattaa aaatattctt
     9541 tggttatgca gctggagtag gaaaaaccta ctctatgttg gaatctgcac aaaatttaaa
     9601 aaaagtgggt gtagatgtag tagttggata tattgaacca cacactagac cagaaacatt
     9661 ggctttactt gatggcttgg aaattcttcc tgtaaaggag atagaatata aatctataaa
     9721 actaaaagaa tttgatttag atgctgcttt agcaagaaaa ccagaagtta tattagtaga
     9781 tgaatttgcc cattctaatg ttgctgggtt aaggcataca aaaagatggc aagatataga
     9841 agaattgctg ttagcaggca taaatgtcta cactactgta aatgtacaac atttagaaag
     9901 tctaaatgac attgttgaga taattacaaa cgtatcagtt aaagagacta taccagataa
     9961 gtttttggat acaaatacac agttagaact tatagatgtt gaacctgatg tacttcttga
    10021 aaggttcaat gaaggtaaga tatataagaa agaacaagct actagagcaa aaaataattt
    10081 ttttataaaa gataacctgg ttgcacttag agaaatcgca cttagaaaaa ctgcagagag
    10141 agttaataaa gaagttcaaa tgaccagact atcaaagggt gatgtaactg taatacctac
    10201 atcagataca cttcttgcat gtataagtcc atctccatct tcagcaaaag ttattagaac
    10261 tgcatccaga atatcagaca gtacatttgc aaaatggata gctttatatg ttgaaactcc
    10321 aaacacagca agacttccat ttgatgaaca aaaacaacta caaaataacc taaaattagc
    10381 taagaaatta ggtggagaaa taattgtttt acatggtgaa aatataatag agcaaatact
    10441 tagaattgca aagctgagaa atgtaacaaa aattgtaata ggtagaaatc attcaagtaa
    10501 taaaagattt agtaaaagat ttaaaaaaga tatagttgac aaattaattg atgaagttga
    10561 ttatatagat atacatataa ttccatataa atttgcctca gatgttaaat acaaacctaa
    10621 aaaggataaa tcatcaataa aatctaaatt taaaattagc aaagttgatt ttataaaact
    10681 tatatttatt actttgatga taagtatttt agcatatgca gtacaaagca tgggatttat
    10741 aagagaaaat atattgttaa tatatatgtt gggtgtagtt ttagtttcta tgtggactaa
    10801 agggtatagt acaggtataa taagttctgt atttaatata gttcttttaa attatttttt
    10861 tacagcacca ttatatacat tatctatagc agattcaaac tatatagtaa cactggttgt
    10921 attctcaata gtaggtataa taactagcac tcttacgtct aagatacagc atgaagcaga
    10981 aactgctgct aaaagagaag aaaatacaaa aatgatatat caaataatta agggattttt
    11041 aagattatca aataaagatg atatagtaaa caaaggtata gaacttttgt ctcttagctt
    11101 aagtagagat gtaatttgtt atttaaatgt agataagcat aataaatcaa agctttacaa
    11161 aaaaaatact aataataagg ataaaaatga tttggatagt gaagatgaaa aagctgttgc
    11221 aagttgggtt ttatccaatt caacagtggc aggaaatgac acggatacac ttcctggttc
    11281 aaagggatac tatataccaa ttgtaggaat gaatttaact ttaggagtag tgggaatatc
    11341 atgtattgat tcaaaattgg atacagaaga tataagctta attgaaacaa taattgctca
    11401 aatggcaata gcgcttgata gagaaatctt gtcagaagct aaagaaaata ccaatcttga
    11461 gattgaaagg gaaagattaa gaagtaatct tttaagagca gtttcgcatg accttagaac
    11521 tccactagca ggaatatctg gtgcagtaag tacaattata aaaaacaagg gcactatagg
    11581 tcaagatata atagatgaat tacttagtgg tgtatatgaa gatactcagt ggttaataag
    11641 acttgttgaa aatttactta gtatgaccaa gatagatgaa gggaaattgg aagttaaaaa
    11701 acattctgaa ctggttgagg aaattatatc agaagcgtta caaaaaataa aaaaaagaat
    11761 tgaaaatgca cttatagata ttaatatacc agaacagata ttatttgttc ctatggatgc
    11821 aaaattaata gaacaagttt taattaattt aatagataat tctttgaaat attcaaagga
    11881 agattgtaag atagagataa atgtatatga gaaagatgat tatgtttggt ttgaagtatc
    11941 tgataatggt cctggtatat caaaggaact taaaaagcat atctttgaca gattttttac
    12001 tggagaagaa ggagctaaag attccagaaa gggtgttgga cttggattat caatttgtaa
    12061 atctataatt caagctcata aaggtgaaat tatggtagaa aacaataaag ataagggttc
    12121 aacttttaag tttagtttgc ctaaggaaaa tgaatagttt taaattttaa aattagtttt
    12181 ggaggaagaa atgagtaaaa aacttatatt gttagtagag gatgataaga caataagaaa
    12241 atttatatct actgcacttt taacacagga ttatgatgtc aaggaggcta taactggaaa
    12301 agaaggtata tctatagctg tctcatattc tccagatgtt gttcttttag atttaggctt
    12361 agaagatatg gatggaatag aggtaataaa agctatcaga caattttcaa atataccaat
    12421 aattgtagtt tcagctagag agcaagatag agacaaggta gaagtatttg atgcaggggc
    12481 agatgattac ttaacaaaac cattttccat agtagagtta ttagcaagag taagagtagc
    12541 atttagacat tcacaagtag aagctcaaca aaaagatgat gttaaatcta cgtttgaagt
    12601 agataaatta ttgattgatt ttgataaaag aaaagttatt gttgatgatg ttgaagtcca
    12661 tttgacacct attgaatata atattctttc attacttgca aagcatcatg gaaaagtgct
    12721 tactcataat tttataataa aagaaatatg gggtagtgtt atagggaatg agactaagtc
    12781 tttaagggtt tttatggcaa cgttaagacg aaaaatagaa aaacaacctg caaacccaag
    12841 atatataatt acagaagttg gtgtaggata cagattaaat gatgaatagt tgtaatatat
    12901 taattattag attatgtgct tattaatata tattaaaatt atatagttta gttaaaataa
    12961 gaatgaagtg tatgtgcttg ttgtcagttt agcacatgca ttttattttt tagctttttt
    13021 agtatcaata attttttagt atcaataata tgaaatatgt tttaaaataa gtatatagtg
    13081 taatctgtaa taaaaacttt ggttaaataa taaatgtatt taacacttac cttataataa
    13141 atttaatata aatattaaat aataattatt aaaatagtgt aaaataatta cagtaaggat
    13201 tataaaaaat ttttagaatg taaattgaaa tttaggagta gatgtatgta tatactgaat
    13261 ttaaatacaa gagagactat tgaagatttt agagataaat tttatgtagc agaaaattct
    13321 tatcttatat taagtgctcc aaaaaattta aaattgctta aggaaacttt ggatatagat
    13381 gaaataactt ttaatgattg tcttaaattt gatgaaataa ccaaacttga tttatttgat
    13441 aattacgatt ttttaagcct aaatacattt gaactaagag atggagaagc tgttatagaa
    13501 gaggtaaata tgtatttatc agataatttt atattggttg tagtgaatga agaacacttt
    13561 ttatttgaat ttgtaaaaaa tattattttg aagaactctc aacttgaaaa aaatccagtt
    13621 attaatctat ttaagataaa ctacttgatt cttagagaag ttataaaaaa tggttttgag
    13681 tctttggaaa aagttgaaga gttaattctt caaatagaag atgaaatgat ggacaacatt
    13741 aataagaatc atgtatctag aataagtgat gtaagaggat taacaagaat tatagttaag
    13801 aatactagac ctcttttgta tattggggat agaatagtta aggaaaatat aagatattta
    13861 aaatattcaa atgtaaaaaa atataattta gaaaattttc aaggtataga ttttggaatt
    13921 gacaaattat acaatttcgc attgtcaaca cgagagttag cagataaatt attagatatt
    13981 tattcttcca gagtaggtga gaaaacaaat aacttaatta caaaattaac cttgttaaca
    14041 gccatatcag caccacttac aattataact ggtatatatg gaatgaactt tagatatatg
    14101 ccagaattaa actggattta tggatatcca gcaactttat tttttatgct atgtataatt
    14161 tttgttggaa ttataatatt taaaataaaa aaattattgt aaattacaga tgatgtttaa
    14221 ttgattttat aaaaatacat caattgagaa gctgatttaa gtaaataaaa aaatatttta
    14281 aattgaaaat atttatagaa aatattgaca attatatttt ctttgttata attattaaac
    14341 aagaagaata ataaattata aaaatggagg ataatactat gaaaggaata ttaaaagaac
    14401 taacagcaaa actttatagc tttttttact ttatgttctg gggcttttat tttagcgctg
    14461 gctatttctt ttacaaaaat atatgtactt cctttctggt gaggccttat tttagtgatt
    14521 ttgagcataa gtaaatttta taaaaatgat tattttatat atttatataa gatttagtgc
    14581 ttgatagatt attttagaga actcattagt gagttctctt ttttatttta aatataaaat
    14641 tataatattg ggaggtagta aaaatgatag tagtattaaa aatgggagca gataaaaatg
    14701 aagttaaaaa attaattgag gctattggga gagaaggagt agaagttaat cccatagatg
    14761 gaacagaatt aactgtttta ggtttagttg gagatactag taaaatagat gccaaaagaa
    14821 tagaagcaaa taaggtagtt gaaaaagtta tgcatgtggt agagcctttt aaaaaggcca
    14881 atagaaaatt tcatccagaa ccttcaatca taaatgttaa tggaatggaa attggttcaa
    14941 agaaaattgc tatgatagct gggccttgtt ctgttgaaac agaagaccaa atagtatcta
    15001 tagctaaaga tgtgaaaaaa tcaggtgcag gatttttgag aggtggagca ttcaaaccaa
    15061 gaacatcacc atacgctttc caaggattga aatatgatgg attggattta ttaaaaaaag
    15121 caaaagaaaa aacagggctt cctatagtta cagaaataat gtcaacacaa gatatagata
    15181 tatttgaaga aaatgtagat gtaattcaag ttggagctag aaacatgcaa aactttgatt
    15241 tattaaaaga attaggtaaa acaaataaaa caatactttt aaaaagagga ttatctgcta
    15301 caattgaaga gtggctaatg tcagcagagt atataatggc tggaggaaat gaaaatgtag
    15361 ttctttgtga aagaggtata agaacttttg aaacatatac tagaaatact ttggatttaa
    15421 gtgcaatttt agctgtgaag aaacttagtc atttaccagt tatagttgac ccaagtcatg
    15481 cagcaggtaa atcatggatg gttgactctt tatctaaggc agcaattgca gttggagcag
    15541 atggtctaat aatcgaagtt cataatgacc cagcacatgc actttgtgat ggaaaacaat
    15601 cgattaagcc aaatgaatat gatgaattga taagtgaatt aaagactatc gcatcagcag
    15661 ttggtagaga aatataatct ataaaaggat ggttaaacgt ggaagaaaaa ctattagtaa
    15721 atttaaaaga aaatagctac aatatattta ttaaaaaaaa tatactaaag aatataggag
    15781 aagaaataaa aaaaatatat tttggaaata agatttttat tgtaactgat gaaaatatag
    15841 caaaattata cttggataaa gtaaaaaata atttaagtga atctggattt gaaattaaat
    15901 ctttaatatt aaaatctggt gaagaaacaa aatcatttaa tactcttcct aaaatatacg
    15961 atagtttact tgactttaag ttaactagaa aagatttgat tattacttta ggaggagggg
    16021 taataggtga tttaggtgga tttgcagcag caactttttt aagaggtata agttttattc
    16081 aaattccaac atctttatta gcacaagttg atagttctgt aggtggaaaa gttggagttg
    16141 accttgagag aggcaaaaac ttagtgggaa gcttttacca accaaaggct gtgtttatcg
    16201 acccaaatgt attattatca cttccagaaa aattctatag agatggaatg gcagaagtta
    16261 taaaatatgg atgtataaaa gacaaaactt ttttcaataa acttttaagt ttaaaaaata
    16321 gagaagaagt aatgcaacat atagaataca ttatacataa atgttgcttt attaaaaaaa
    16381 ctgtagttga agctgatgaa aaagactttg gagatagaat gcttctaaac tttggccata
    16441 cattaggtca tgctattgag aaatactaca attttacggg ttatacccat ggagaagctg
    16501 ttgccatagg aatgtataag ataactttag aaagtgaaaa gataggaatt acaaaaaaag
    16561 ggacatcaga acttataaaa tcaattttaa tgaattataa tttgccttat gagattgaat
    16621 taagagataa taaatctata gtagatgcta tatctcttga taaaaaaaat ctaggtgata
    16681 cattaaaaat aatactttta aaagatattg gagagagtat tatatataat actacttcta
    16741 aattttttca ggaggtaaga taatgggaag tctaaaaata tatccaagta aattatctgg
    16801 agatgtaaaa ataccaccat caaaaagtat ggcacatagg gcagttattt gttcatctct
    16861 aagcaatgga aaaagtagaa tttctaatat agatttttct gatgatataa tagctactat
    16921 aagagcaatg acatcattag gagctattat agaaaagaaa gaagatattt tagaaatttc
    16981 gggaattttt tctaaagaag gtattttaaa tagagagaat cagttgaatc aacctaaatt
    17041 gacaatagat tgtaatgaat caggctctac attgagattt ttagtaccta tatctttagc
    17101 atttgatggt gtaaaaaggt ttataggtag aggaaatctt ggaaaaagac cacttgatac
    17161 ttattatgag atatttgaca gacagaatat aaaatatagt tacaaagaaa atcagttaga
    17221 tttgattata tcaggtaagc ttaaaccaga tgaatttaga gtaaaaggaa atataagttc
    17281 tcagtttatc acaggtttac tgtttatatt gcctacgcta gagagtgatt caaagataat
    17341 aattactact gagctagaat ctaaaggata tttagattta actctttcaa cgataaaaga
    17401 ttttggggta gaaattataa acaataacta taaagaattt attataaaag gaaatcaaac
    17461 gtataaagcg agagattata aagtagaagg agattattct caaggagcat tttatttatc
    17521 agcagatgct ataggagaag atattagcat attagacctt aaagaagatt ctcttcaagg
    17581 ggatagtgaa gtagttgaaa ttttatctag gatgggaatg gaaatattaa gagaaggcaa
    17641 caaaataaaa ggaataacaa atggtttaaa tggtactttg atagatgcat ctcaatgccc
    17701 agatataata cctgtattat ctgtagtagc atccttaagt atagggagga ctacaataat
    17761 taatgctggt aggcttagaa ttaaagaatg tgatagactt catgcaataa atgttgagtt
    17821 aaataaactt ggagcaaata tagaggagaa agaggatagt ttgataatcg aaggagtaag
    17881 caagttaaat ggtggagtag aggtgtggag tcacaaagac catagaattg ctatgacact
    17941 tgcaattgct tcttgtagat gtgataaacc aataatatta aaagattttg agtgtgtatc
    18001 aaaatcttat ccacattttt ttaaagattt taagatgtta ggaggtcgaa ttgatgagtg
    18061 gaatatgggg aaataattta aaagtatcaa tttttggaga atctcatgga aatgctatag
    18121 gtataaatat agatggtctt ccttcgggga ttgaacttga ccttgataat attgataaag
    18181 aaatgaagag aagagcacct ggaaaaaatt ctatatctac ttcaagaaat gaaagtgata
    18241 taccagaaat tctaagtgga tattttaatg gaagaactac aggaacgcca ttatgtgcaa
    18301 tcataagaaa tagtgatact cgttcaaaag attatggaga gcttaaaaac ttaatgagac
    18361 caggacatgc agatttcaca ggtaatgtga gatattctgg atttaatgac tatagaggtg
    18421 gaggtcattt ttcaggtagg ataacagctc cattagtatt ttgtggggct atatgtaaac
    18481 aaattttatc acaaaaagga atagagatag gagcacacat aaaaaaaatt aaaaatatag
    18541 aagatatgag ttttgattat gtaaatattt caaaacaaca gttatcaaat ttgcaaactt
    18601 tggaactacc tttacttgat ttatcaaagg aagaagccat gaaaaatacg attatagatg
    18661 caaaaaatca aggagattca gttggtggta taattgagtg tgcagtagta ggtattaatg
    18721 tagggcttgg aaatccattt tttgactctg ttgaatctac tttatcacat ttattgtttt
    18781 ctgttcctgc tgtaaaaggt gtagagtttg gtttaggttt tgaacttgca gatatgtatg
    18841 gctctcaaag taatgatgaa atgtattatg aaggaaatca agttaagagc aaaacaaata
    18901 acaatggagg aattatagga gggattacta ctggaatgcc aataattttc aaagtggcaa
    18961 ttaagccaac tccatcaatt agcagacaac aaaacacagt taatataaaa gataaaaagg
    19021 acgatatttt atatattaaa ggcagacatg acccttgtat agttcaaagg gctatacctg
    19081 taatagaagc agtaacagcc attggaattt ttgatttgat gaaaggacga taataatgga
    19141 ggatttaacg agatatagaa atgagattga caatatagat aaagagctta ttcaattatt
    19201 tgaaaagaga atgaatacag ttcttgaaat tgccagacat aaaatgaaaa ataacactac
    19261 tatacttcaa aaagatagag aagaaaaagt actaagtaga gccgtagata atttagataa
    19321 taaagcatat tcacaagaaa cggtacagtt ttttaattct ataatggaaa ttagtagagg
    19381 attacaaaaa agatttatag ataatgatgg tgagcagaaa atcgagttta gtttagattc
    19441 atcaaataag aattttgatt tgtcaaatgt aaataaatat aagtctttaa aagatgaatt
    19501 aaataaaaaa aatatcctag taggctttcc tggaaaatca ggttcattta cagaagaagc
    19561 actaaataaa ttcttcaaca aaaaaatatc aaaaaaacaa tttaaagaat ttgaagatgt
    19621 tttcatagca cttaaaaata aagaaataaa ctatggaata atccccatag aaaattcatc
    19681 tacaggagct atctctgaaa cttatgacct tttgagaaag tacggttttt atatagttgg
    19741 agaagaatgt ataaaaatag accaaaattt aatagggata aaagacacaa agcttgaaga
    19801 tataaaagaa atatattctc atccacaagg tataggacaa tgtagtgaat ttttaaaaca
    19861 aaatagtaca tggaaattga taccatttaa taacacagca acaagtgcag aacttgtaag
    19921 aaaacttcaa gataaaacaa aagcagcaat tgcaagcaaa aaagctgcaa gtatttatgg
    19981 attagaaata ataagtcctt gtataaatga tatagcaaac aactatacaa gatttgtagt
    20041 aatcagtaat caaatacaca ttgaagaaga atcaaacaaa atgagtgtag tattttctgt
    20101 tgaacatgaa gcaggaaaac tttataaagt acttggatac tttgctaaaa ataatataaa
    20161 tatgacaaaa atagaatcaa gacctatgaa aaatgcctca tggagatatt tcttttatat
    20221 agattttgag tgttctatat acaatgctaa agtgtacgat ttattagaat tgatagaaca
    20281 taatacagca tattttaaat ttatgggagt atataaaaat aaaactgaat agataagata
    20341 aatttttatt taatttataa ttattaggag gtgtaagtat gaattttttc ggtctagtag
    20401 gtgaaaaact ctctcacagt gtttcacctc aaatccacaa gagagtattc gagatattga
    20461 atattgaaag tgcttataaa aactttgaaa tttcaaaaga agatatatct aagctagacg
    20521 gtgctataaa attattagga atacaagggg taaatgttac tgtaccatat aaggagcgta
    20581 ttatgaaata tcttgatttt atatcaccag aggcaaaacg tataggagca gttaatacaa
    20641 ttttactcag agaaaatatg ttatatggtt acaatacaga ttactttggt cttgatagta
    20701 tgtttaaaat ggctaatatt gatgttcaag gaaaagtagc agttatatta ggaactggag
    20761 gtgcttctaa agctgctctt acatatttta ttgattctgg tatagaaaag ctttatgttt
    20821 caactagaaa aaaggatgat aaaaaattat taaatagtaa agctatatta atagattatg
    20881 aagaacttaa acatataaaa ggagatataa tattaaatgc tacaccagta ggtatgtacc
    20941 ctaatgtagg aatttcacca gtgtcaaaat ctattataca aaattttgat atattaatag
    21001 atttaattta caatcctgga gaaacagagt ttttaagaat tggtaatagt atgggtaaaa
    21061 aaacttgtga tggattatat atgctggtgg ggcaagcaat aaaatctcag gaaatttggc
    21121 aagatactaa aatagataat agtattttag atgttatata taatgaacta aaattagaat
    21181 ttttgtaagg gagattgtta tgataaataa aacaaaagaa aaattgatat taataggtat
    21241 gccaggtagt ggaaaaacta caattggtaa acttttggcc aaagaatata attgtagttt
    21301 ctgtgatatg gatgattata taattcaaat atctcaaaaa agtatagcag agttgttttc
    21361 agaaggagaa gatatcttta ggaactatga gactcaagca tgtagagaac tttctataag
    21421 tgataaaaca gtgatatcta caggtggagg tgtaataaaa aaggatgtta atatggaaat
    21481 actaaaagaa acaggtataa taatttttat tgatagacct atacaaaaga ttttggaaga
    21541 tataaatatt aattcaagac cattattaaa aaacggaaaa gatagattgt ataatcttta
    21601 taatgagaga ataaatcttt ataaaaaatt ttcagatata gagattttaa atgataaatc
    21661 attaaataat gctgtatata atattacaaa tgcagtcagt gaaaatttta aatttgactt
    21721 taaagaaaaa taatctaggg ggaagtaaaa ataatgaata ttgttattgt aggactagga
    21781 gttataggag gctcttttgc taaagcatta aaaaaagctg gatatgaaaa tgtatttgga
    21841 gtagatgtag atttagaaac tttaaaaaaa gcagaaaaag caaaaatcat aaaaaaagga
    21901 tgtactacag gtaaagagtt atttaaaaaa gctgatttaa ttatactatc tatatatcct
    21961 agattagttg tagattttct aaataagaat aaaaactttt ttaaaaaagg gactataatt
    22021 actgatacta caggaataaa agaaacactt atcaatgatg tgttgcaaat tattccagat
    22081 gatatagatt ttatatttgg acatccaatg gcaggtaggg aaaaaaaggg tattgatttt
    22141 gcaagtgaac aagtttttaa tggtgcaaat tatataatta ctccaactgg cagaaacaac
    22201 ataaaaaatt tagagttagt tgaaaattta attttagaga ttggatttaa aagagtaaaa
    22261 aagttaactt cacaaaaaca tgatgagatt atagctttta cttctcagtt acctcatgtt
    22321 atggcagttg ctcttataaa tagtgatgaa gaaggtagag atacgggtaa atttattgga
    22381 gatagttata gagatttaac tagaattgca aatatgaatg aagacttatg gagtgaactt
    22441 tttttaggaa atagagataa tttactaaaa gttatagaaa actttgaatc agaagtaaat
    22501 ttagtaaaag aagctatatt taacaatgat aaaaataaat taatagagta ttttaaaaaa
    22561 tcatcaataa gaagagaagc tttagaaaaa taaatattaa tatttaagct atatatagat
    22621 gggtataaat ataagcgata gtgtatttaa ttgtattaat aaatatggat tcaatataat
    22681 taaatatgct attgatatat ttgtaccctt aactattgtt atcttttaaa tgataatgat
    22741 atttatttaa atactcttta ttaacaattt ataaaactta attgtaaaaa atacttatta
    22801 atagtaaaat aaagtgaatc aattatatta taattgctaa agagagtgag gtgtagctat
    22861 ttgaaaaaaa atagattaat agcaaagaat atatcaatag catttatagt actattcttt
    22921 tttagtgtat ttacattttt ttatgtaggt aatattaata gagtgttaga atatgaaaca
    22981 aatgacataa taacagttac aattgctgga tggattatac tttcattttt atttttaggt
    23041 attataattt acatattata ttcaaaagca aatagtcaaa aaactataga aaaagtagca
    23101 tatacggatt ttgtaacagg ttattccaac tggagaaagt ttgaattaga tgttacaaat
    23161 ttattaaaga aaacatcaca aaataataaa tatgcaatgg tcatattcga tattgataaa
    23221 tttaaagcaa ttaatgatat atatggccat aaaaagggaa atttaatatt aaaagatatt
    23281 gcagatactt taaatgaatt gactgatatc aatgaaactt ttgctcgtgt aagtgctgat
    23341 aatttcaata tattattaac atataacaaa aaagaggata taataaatat tatcaaaaag
    23401 attatggcaa ataatgaact tgtaaactta tcttttggaa tttatgaaat caaagataag
    23461 gatttatctg taagtgtata tagtgataga gcttcacttg caaaatcatc aataaaaaat
    23521 aatagtgatg taaattttgc attttttaat gacaaattaa gagagaaatt attatttgaa
    23581 gataagatag aaaaagaaat ggaatatgct cttgaaagtg ggcaattcgt aatgtattta
    23641 caaccaaaat acaatattaa attagataaa ttttgtggaa gtgaagccct agtcagatgg
    23701 caatatacag aaaaagaggt catatatcca ggtgatttta ttcctatatt tgaaaaaaat
    23761 ggatttatta gaaagattga tatgtacata ttagaacaag cttgtaaaga aatccggtct
    23821 ttatttgata aaggaatttc acctttacct atatctgtaa acttctcaag agttgatttt
    23881 tttaaaaaag attttattga aaacatagta aatatatgtg atagatataa aataccatat
    23941 agtttaatag aaattgaaat tactgaatct agtatgtttg gagatactga tactctattt
    24001 aatgttagta gaaacctaca ggatattggc tttatagttg caatggatga ttttggctct
    24061 ggatattctt ctgtaaatat gcttaaaaat ataccattaa atgtaataaa attagataga
    24121 ggattttttg ttgatgataa agatgttgat aagagtcaaa ttgttataaa aagcatagtt
    24181 tctctcatta agcaattagg aataagggtt gttgctgaag gaattgaaac aagaagtcaa
    24241 atagaaatgt taaaaaaagc aaattgtgat attgttcaag gatattattt ttcaaaacca
    24301 ttaccaatta aagaatttga aaaattagtc tataaaatat agtcagcgag tcttaaatgg
    24361 gaggtggtga ttaagtgata agagcaaaat cgttaaaaaa tgtactgagt attgttttaa
    24421 tatttttatt tacaataatt tctttttctg attttgcatt taaaactaat gtccaggaag
    24481 aagaaaatag taaaattaat ttaagagaaa tatccctaca aagttccaat gctataaaaa
    24541 taaaattaga tagttctcta tcctttatta aaagtgtggc aaatatggtt tcttatttta
    24601 atactataga taactcaaaa atagttccat tattagctaa tatatcaaaa gaatctgact
    24661 ttcaaagaat ctctattgta tctcctgatg gaataagtca taccagtgat ggcaaaatta
    24721 tcaatgtgtc aaagtatcag tattttaaga aagctattaa aggagaaagt aatatatctg
    24781 aagttgtgaa atctccaatt gatggaaaag atacttttgt tatagctact cctgtattta
    24841 ataagaataa caaaataatt ggtgttttat acggttctta ctacaatgat aaattagctg
    24901 aatttataga tataacaagt tttaatagtg aagggtacat agatatattt gaatctagtg
    24961 gtcaatttgt tttaaaatct tcacatacaa acaaactgat taagtcaagt agtaacatat
    25021 atgatatatt taaaaaagct tcttttgatg atggttatag ctataaaaaa atatatgaag
    25081 atacaaaaaa taacaagagt ggctttacct catatgaata caaaggagaa aatcgttatg
    25141 ctacttatac acccattggt gtaaatgact ggtatgtaat ttctgtgata ccacaatata
    25201 ctgtaagaag aaacattgat aaaataaatc aattagcatt ttctcttatt ttcaaggtat
    25261 tcttattgtt tattatgctt ataatatata ttatttataa aaacaataaa tctcagaaag
    25321 atatactaaa agctaacaaa gaaatctcat ctattacaaa tagcattcct ggaggagtac
    25381 aaaagtgtac cctgggtgag acatgcaata ttaatttttt aagtgatgga tttatagagt
    25441 tgtttggtta tacaagagaa gaaataaaaa cattgtttaa taatgatttt tatagtttaa
    25501 tacataaaga tgatgttgaa agaataaaaa aagaaatttt taaacaactt tacaaaaata
    25561 aaactgttga attagaatat aggattataa caaaaaatgg taatacagta tgggttttag
    25621 ataaagggga gcttataaca gaacctgatg gaaaagagta tttttgttgt gtaactgtag
    25681 atattactag aacaaaacaa ttagaacaag agatggaaca aaaaaatcta gaacttgaaa
    25741 tgatatcaga aaatgtttta ggtggtataa ttataacaga atttaatgat gattttacta
    25801 taaaatacgc taactctggt tatctttcaa tgattggata tacacgtgaa caattagaaa
    25861 atgaacttaa atacaaagct tgtaatcttg ttttttatga agatagaaag aaggctcttg
    25921 aaagtataca acatcaatta aacacaaaag gaatagctgt atatcaacat agaataagaa
    25981 gaagagatgg taatattatt tgggtttcta taaaaggaag gcttattgtt gatgatgatg
    26041 gtattgaaaa aggtatttgg attctaaatg atataacaga tttaaaaaat gcagaacaaa
    26101 aacttaaaat aaatgaagaa agatttagaa ttgcattaaa gcaaacatca aacactatat
    26161 ttgagtatga tataaaaaca aagtcacttt taaatactag taaagagctt gataaatata
    26221 atatttataa aattactaaa aatgttccat attctctagt agaatcagga gttattcata
    26281 aagattttag tgatgatttt ataaacatgt atgaaaatgt aataaatgga gcaaaaaaag
    26341 caagttgtgt agttaagatg aaacttccag atgaaaatta tatatggaat aaagttacct
    26401 tgactactat ttttgatgaa gatggtaatc ctgtaagagc aattggtata caggaagata
    26461 taagcaaaca aaaggaagca gaaataagat acaatcaaga aggtaagtat cattcagcta
    26521 tattgtcgga agcaatgata acctatgaaa taaatgtatc aaaggatact tttatcaaag
    26581 gaaatgaaag ttggcaaaaa ccatttggaa ttaagcctga caataactat tctcaaatgg
    26641 taaaaactat agcaaatagt attgtacata aagacaataa agaaacattt ttaaatacat
    26701 ttttatgtga aaacttattg gatgcatata aaaataatgt gactgaaata aaattagaat
    26761 atcaaagaat gaatcaagaa aataaaatga tttgggtcat ttgtacaatg cacttaataa
    26821 aagattctat tacgaacgat ataaaagcac ttgcttatgt taaagatatt gacaagcaaa
    26881 aaaatgaaga aattagatta aaattaaaag ctgaaagaga tactcttaca ggattatata
    26941 ataaaggtac tacagaaaaa ttgattaatg atttcttgaa ctctgaggaa gcaagcttag
    27001 gaatgcatgc atttttaatt gttgatatag ataattttaa atctgtgaat gataatttag
    27061 gacatgtatt tggagataaa gtattatttg aagtttctaa aaacctaaaa cctatattta
    27121 gaaaagatga tatagtaggt agaattggtg gagatgaatt tatagtattc ttaaaaaata
    27181 ttcagtcttt tgagaatatc aaaaagaaag cagaaaaaat atgcgatata tttagaaatt
    27241 cttatacaga aaataaaaag acatataaaa tatctggaac agttggaatt tcatttgtac
    27301 cagaacatgg aactgtattt gaagaacttt ataaaaaagc agacatagca ctttattatg
    27361 caaagagcaa aggaaaagat aagtatgcac tttataatga ttgtatctac caaaatgaac
    27421 ttgaaaataa tataagtgat aataatataa agttagcaga taaatgtgag aaatccaaac
    27481 cagtacttaa agaaattctt gattctattg ataattatat ctatatagtg aatccaaata
    27541 cttatgaact tctttttgtc aataataagg tgtctcgttt ggatttagag ataaaattag
    27601 gagaaacctg ttataaatca ctattaaaat ctgatagacc atgtaataat tgccctctta
    27661 acggattaga agaaaataag cattcaaaat tcaaaataga gacaaataca tttggtaaaa
    27721 atatcagtat agcaagttgg attaattgca tagatggaga atacaactgt cttatagagt
    27781 gtatagattc ttctttatac aaaaataaat aaaaaaggac tatgaagata tgttaaatag
    27841 tataactttg atacttggta taatatttac aatttactat ttttatctca aaatagtttt
    27901 tggaggaatt tcatttagtg agattttctt agttgtagga attgtactaa ttatctatca
    27961 aatatttaag aaaaaaatta aagaaaagaa ggttttttat agggtgttaa aaataataat
    28021 cagcattttt ttaatagtat ttgtagttac tgagagtcta attatttttt atcccaaaaa
    28081 tagtttagaa tcaaaaagtg attatctctt aattttagga gcttctgtaa aaaagactac
    28141 accaagtaca accctgaagg gtaggttaga cactgcattg aaatacctaa aaattaatga
    28201 taattgttat gtggtagtat ctggtggtaa aggaagtgga gaaaaaataa ctgaggcaaa
    28261 agctatgaaa gattatctta taaaaaatgg tatagataaa aataggataa tagaagagga
    28321 taaatccacg aatacttatg agaattttaa atattcaaaa ttgaagatag aagaacatag
    28381 tcaaagaaaa ctaagcgatt taaaagtaaa aatagtaaca acagattttc atgttctcag
    28441 aagtaaaata ttagcatata gaaatggata taaaaatacg tctttttatg ctagtaaatc
    28501 aaaactttca tttgtaccaa cttactatac aagagaattt tttgcattat ggaaaacaat
    28561 catttttgat agataataaa taaaaagact agatataaac tcatatctag tcttttctat
    28621 tcccttaatt ctaatccatt ttccaacttt acttgttgta gaaatttcaa tattgggatt
    28681 tacaagtaat tgcttataag aagttttata tttttcgatt ccaaaatata attttccttc
    28741 ataatccatt ataaatccaa atggtcttac tcttggttta ttatcttcaa ctgtagcaaa
    28801 atagaatgtt gagttttctt ttaaaaaatt cattattttt tcatgattaa atcctctttg
    28861 tatcttatac taatataatg aatataaata aaatattcat tatacttaat ataaatcgat
    28921 ataaaaacaa ttacaatatt atattgatta ttcgaaatta tatataaaat tcagaaaaaa
    28981 ttttagttta cataacaaaa ataaagtcaa ctaaaattat tgattttaga ttaaagtacc
    29041 ggattaagca tttaagtaga ttttaagctt gatttgattg gaaaggtaca aactatcacc
    29101 gaaaaaaatt aaacccattt aaaaagcaat ataaaagctt gttatttttg agaaaaatag
    29161 actctctaaa ctcaattaga ttaaaaataa aatacagaaa attatatctc taaatatctt
    29221 aaataattaa gtttaaattt tacgatattt tgtttgatta tttataatat aaaagcatac
    29281 tcttaatctt caaaatttaa aaagagtaaa aaataacaaa aaaatcactg acatttcact
    29341 gacaaattgt aattttgtta ttataaatac attgaaaata cttgattatt gaaaaatggc
    29401 ataaaataat acattttatg ccatttacta ttatttgata gtttttaact tatgacataa
    29461 gatatttttt aacacataca ataaaatata aaaaaattga tattgcaaat aaaaaaaata
    29521 catattagat gttatagaaa aaaagcgcac ttataggctt aaaatgtcac ataaaagagt
    29581 tttaaagaat aatatattat gaacacaaat tttgtaatca caaaggagtg ataatatggc
    29641 aacagtatca ggacaaatag tttttgatag aaatagaagt gctaccattg attctggaga
    29701 ctcaggaata gccaatatac cagttgtatt acaagatata gcaactggag ttagacttgt
    29761 ggttctcaca gatgcatcag gaaattatgt ttttactaat gttccaaatg gtaactatag
    29821 aattgtagaa gcttttggaa ctacaggagg agtaccaaca ccagggaatt ttgcaacagc
    29881 gatagtaggt ccagttccaa ttgcagaaat gccacctatt agtcctccta ttggttcaac
    29941 aaacttagat ggtacaacac ctaacacact attaataaca gtagcaggtt cagatataac
    30001 aaatcaaaat atattaaatg ggcctgtgat ttatacacct atagagaata tattggattc
    30061 ttgcgtatct gtatctaata taaaccttat tacagatgca gataacggaa cattcggttt
    30121 ttttccacca ggtacaccaa gtaatacagg acctgaaata gaaccttacc caaatgtgac
    30181 gccagatttt acgtatgttg taccagaccc aactaagttt acacctttag atggagaata
    30241 tacagtacaa aatattatga caaatgcaat gagtaatgtt ataggagctt ggtggcgtat
    30301 tgctgaccat actacaggaa atgaaacagg aagaatgatg gtggtaaatg gttttaatcc
    30361 tggagctgta ttttttaaat caacagtaca agttacacct aacactaatt atttatttag
    30421 cacttggatt ttaaaccttt ttaaggtaac aggatatcca ccaccacagc taggtgtaag
    30481 aatatttgac caaaataata atatactata tcaagcaaca ttaggagctc aaattccagt
    30541 taatacaaac gcaccagaat ggaaagaagt aggtactgtg attaattctc aaaataataa
    30601 tcaacttaca gtagaatttt ttagtgaagg agaaagcgcc attggaaatg attatgctat
    30661 agatgatgtt tctttaagag aaatagctgt tccaactttt actccaataa aaacaagtaa
    30721 cacagaaact gctaatgtag gtgatataat cacatataca gttacattag ataatacttg
    30781 tgatagccct ttaactaatg tattttttag ggatttaatt ccaaatggtt tagagtttat
    30841 acctggaact gtaacggttg atggagtatc tgtaccagga gtagacccta atacaggttt
    30901 ctcacttcca gatattggag gaggaacagg ggttgaagta acttttgatg ttgtggttga
    30961 aagtattcct aacccaaatc ctactaataa tatagctaat atagattact cctatacacc
    31021 tgtagaaggc ggtataccaa atgatttttc agtagattct aatccagttc ctgttgaagt
    31081 aatatcagca gatattgaag ttacaaaatt atctgaacct actatagtga atccaggaga
    31141 ggaattaata tacactataa aagtagtaaa taatggacca ttcccatctg aaaatgtggt
    31201 tttaacagat gatgtaccag cttctattat aaatccagag tattcattag atggaggagt
    31261 aacttttcaa ccatggactg gaagtttaaa tatagggaca ttagaagtag gagagacaag
    31321 agttattata attaggggta ttgtaaatcc atcaacagta ggaattataa caaatacagc
    31381 tgtagtatct tcaactacag cagacccaaa ccttaataat aatacatcta caatagagac
    31441 agaagtgaat ttattagcag atattttagt aatgaaaaca gcagaaccta attcagctgt
    31501 tccaggtact ttattgagat atactattca agtagaaaac ttaggaccag caaatgctga
    31561 aaatgtaata ttaaatgatg atattccagc ttctattata aatccagaat attcattgga
    31621 tggaggagca agttttcaac catggaatgg aagtcttaat ataggaactt taaactctgg
    31681 aatttcatta acagttttaa ttcaaggaac agttagttta aattcttcag aatatatagt
    31741 aaatacagca acagtttctt caactacacc tgacccagat ttaagcaaca atatatctac
    31801 cattattaca ccagttaacc cacaagcagg tatttctatt ataaaagtag ctgacgagga
    31861 tgtagctgtt ccaggagaag aatttgtata tacaattgaa atttttaatg aaggaccatc
    31921 taatgctacg aatgtagttt taacagatga tataccagat gttattttaa atccagaata
    31981 ttcattggat ggaggagcaa ctttccaacc atggaatgga agtcttaata taggtacagt
    32041 tgcaccaggg caattaataa gaattatcat aaagggatta gtaagttcaa ctgctacagg
    32101 agatattact aatacagcag aagtaagtgc agatgtacca gaaccagtta cggattcatc
    32161 aacagtgaca actcctatag ttccatcagc agatatagaa gtaataaaaa ctagcaatat
    32221 ggatacagca gttccagaag aaactttcag ttatactata acagttacaa atttaggacc
    32281 atctgctgca caaagtatcg ttttgacaga tgatatacca gatgttattt taaatccaga
    32341 gtattcattg gatggaggag taactttcca accatggaat ggaaatctta gtataggcac
    32401 tttggatgct ggagaaataa gaagtattat aataagagga acagtgagcc agacagcagt
    32461 aggtacaatt ataaatacag caacaataag ttcacctaca cctgacccaa atccagataa
    32521 taatacttca acagatgaaa cagatataag tccattagca gatatttctg tgataaaagg
    32581 caacgaacca gttgctatac caggaggaag atttatttat ggaattgaaa ttgcaaatgc
    32641 aggaccatct tttgcagaga acgtaacttt aacagataat ataccagctt ctattttaaa
    32701 tccagagtac tcaatagata atggagtgac tttccaacca tggaatggaa gccttaatat
    32761 aggtacttta gatgctggag agataagaaa tatcataata aggggaacag taagtcaaac
    32821 agcaataggt acaattataa atacagcaac agtaagttca actacacctg acccaaatct
    32881 aaataacaat acttcaacat cagaagcaga ggtaagttca tcagcagata tttctgtagt
    32941 aaaacgaagc aatcagactg ttgttgtacc aggagatgta ctagactata caattgaggt
    33001 gagaaatgca ggaccgtcta ctgctcagaa cgtaacttta acagataata taccagcttc
    33061 tattttaagt ccagaatatt caatagataa tggagtgact tttcaaccat ggaatggaag
    33121 tcttagtata ggcactttag atgctggaga gataagaaat atcataataa ggggaatagt
    33181 aagtcaaaca gcaataggta caattataaa tacagcaaca gtaagttcaa ctacacctga
    33241 cccaaatcca gataataata cttcaacatc agaaatagat ataagttcat cagcagatat
    33301 ttctgtaata aaattagcaa ataaaacaga agcatgtgta ggagagcaaa tagattttgt
    33361 aatagtagta tcaaatgcag gaccagaaaa tgctcaaaat gttactttaa tagacaatgt
    33421 ttcagataag cttaagaaag cagtattttc acttgataga ggtgtaagtt tccaaccatg
    33481 gactggaagt cttaatatag gtacactacc agctggaact ttaagagtga ttatattaag
    33541 aggaattata aaatcaacat gtcttgatag attaacaaat attgcagaag ttacttcaac
    33601 aacaccagat tcaaacctta acaataatat atcaagagtg caagtagaaa taaaacaatt
    33661 ttgttgtaat gattgtggtt gtaaagattg ttgctgtaat tgttgttgca atgattgtgg
    33721 ttgtaaaaat tgttgctgta attgttgtaa aaatgacttt tggtgttgat tttataaaat
    33781 aattttgtat accttaaatt aaactgaaat aaaaaataaa ttcacatatg agtaaaatat
    33841 tttataaata ttaataaatt ttctatcttt gatatggacc acattcgtaa tactagttat
    33901 ta
//
LOCUS       DAMJVI010000034        33473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.036, whole genome shotgun sequence.
ACCESSION   DAMJVI010000034 DAMJVI010000000
VERSION     DAMJVI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.036"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..1084
                     /locus_tag="PC545_001704"
     CDS             <1..1084
                     /locus_tag="PC545_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2456475.1"
                     /translation="VLSIVSTLLVYLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINM
                     GLSSLITIIIAGFIYWYSSKKFNIKVMNFDKYIAILMIPLLLIILFMQSFEYSSNINI
                     DTSLGIVKLLLNTSITEEIQAFLFSIIGTICVFFSLFTFKKLIQALEDDKERAIMNQQ
                     IHAQKNYIEEAKSRLSQTISFRHDFNNHLAIVNGLLKKDQILKAQDYLNKLEKVTSDL
                     SFSCNTQNIVIDALFNNKLSIAKQLGIEVDCEVIIPQSTNMNDFDLCIVFANAIDNAI
                     KACRVVDKQNRYLKLSTKLEGGFFMIEIKNSYNPINNYTSGSGIGLLNIKEIAKKYQG
                     AISIDKTSDYFQINVLLVISLHCASI"
     gene            1123..1608
                     /locus_tag="PC545_001705"
     CDS             1123..1608
                     /locus_tag="PC545_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456476.1"
                     /translation="MYLNRESLIYRNEEVTKMKVGNINGQAPITNGLDSCQSEVQGLE
                     QKKKSLQDEIEKVRSDKSIDGETAKLKIEALKKEMEEIENKIQELKGNKAKKTNKEEN
                     NVSEVNNNLLERKKEPSNNENVKQLYDVFERSNVDEQWDNTYRVLQDDKNLKVKFNRP
                     I"
     gene            complement(1871..2107)
                     /locus_tag="PC545_001706"
     CDS             complement(1871..2107)
                     /locus_tag="PC545_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456477.1"
                     /translation="MLLTHIYFNGQFKQTIELYKQAFNATVKTLIEDSCYLLIVHNEI
                     VIHNKFLILNDFGNKNGVSISEGYQPTDKIQTAS"
     gene            complement(2262..2486)
                     /locus_tag="PC545_001707"
     CDS             complement(2262..2486)
                     /locus_tag="PC545_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2456478.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(2583..3836)
                     /locus_tag="PC545_001708"
     CDS             complement(2583..3836)
                     /locus_tag="PC545_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HDF2456479.1"
                     /translation="MITLSFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYKLKPSSNIDLSIPRYNRNLKRFGSKFFRNK
                     NRIKNNNFSETITPSVFLEQVLLIFICINTGEIISRCFYITFNILLPSVVTCMFSAVI
                     FRNLNDKINILELNFKLIDFLKELSLGIFLTLSLMNIDLFELSTLLPPILLIVTFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGDSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
     gene            4096..5085
                     /locus_tag="PC545_001709"
     CDS             4096..5085
                     /locus_tag="PC545_001709"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDF2456480.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDVFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDHKKAYNLVKDKKILLELCPTSNVQTKTVDSYEAHPFYTFYKDNLHVSINT
                     DNRTVSDINLSSELNVIFDTFKLGLEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            5157..5897
                     /locus_tag="PC545_001710"
     CDS             5157..5897
                     /locus_tag="PC545_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HDF2456481.1"
                     /translation="MKDYEDVVYIKLVKKELEEWKKNILKKPSIFDKASKGIQNKFNG
                     VLPDKYHEIITSSIKVMTKTVLFGYKYATKPPLRNMSLEEREKLVLEKAKTYKTTAMI
                     EGAGTGAGGILLGLSDFPLLLGIKVKFLYDVASIYGYDVKDYRERIYILNILQLAFSS
                     QQHINKTFKLMNNWDEYVNSLPEDINDFDWRTFQQEYRDYLDLAKFLQLMPGIGAFVG
                     FYVNGKLIDKLCETAVSAYRLRIRELKL"
     gene            6113..7264
                     /locus_tag="PC545_001711"
     CDS             6113..7264
                     /locus_tag="PC545_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2456482.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRTAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVQKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            7289..8044
                     /locus_tag="PC545_001712"
     CDS             7289..8044
                     /locus_tag="PC545_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HDF2456483.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGIYFLNKNIRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAKGYEPVNISSM
                     CTVFAESEIVSLLAQNISTGEVAAGILKSIANKSTSMLARGEVIDEVAFTGGLAKSKE
                     LVKMIEEILGKKIFIAEDTQIIGALGAAVIGFR"
     gene            8236..10623
                     /locus_tag="PC545_001713"
     CDS             8236..10623
                     /locus_tag="PC545_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HDF2456484.1"
                     /translation="MKKFTSKKVTRFVALFLISILVMSTIPVSADNSNTLNQIKKAEY
                     SETYKQYLNDAKNGCTEKYNGIIPNPYQLEGTQIQSKGRTAPTSYDPRKLGLMTSIKN
                     QEDLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMNGALNYE
                     AMGYLTSWSGPKLEKDIPYNGRVSKAQGAKKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSGYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKDNFNANI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGKKPNDDEKMYQHD
                     YASIVPLISKKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTAQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSNFYPCNFTIKAVTEKSSGESIPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGSSIVDALAATPFTSAINSPILLTQKEALNSKTKAEIQRLG
                     AKKVYLIGGENSISKEIEQQLKSLNISIERISGSDRYKTSLLLAQKLNGIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNAFVVKNGIKNQNDLIDGLAVG
                     PLGAKTGSPVILVGDKLADSQKEVLKNKTLEKVTQVGGGANKNAFKELVKLKSAK"
     gene            complement(10854..11087)
                     /locus_tag="PC545_001714"
     CDS             complement(10854..11087)
                     /locus_tag="PC545_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2456485.1"
                     /translation="MATTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            11376..11966
                     /locus_tag="PC545_001715"
     CDS             11376..11966
                     /locus_tag="PC545_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456486.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMEQYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRVDESITCEQLTDIFMKFYRMV
                     DSTSILNLMINGEFEILVRKLPDSIIEEYFRHDDFEIGEIISCIPNAKNKDIESFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            12080..12925
                     /locus_tag="PC545_001716"
     CDS             12080..12925
                     /locus_tag="PC545_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456487.1"
                     /translation="MIKVDDLSFSYTDRDFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYGGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDELLKSVGLENESNKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVRYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            12922..13605
                     /locus_tag="PC545_001717"
     CDS             12922..13605
                     /locus_tag="PC545_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456488.1"
                     /translation="MRLKNLILGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMFIAITLNLDNIIISTIGTFFSSIIFSLLGLIIASKASSLNQFIVLSIPIEIICFI
                     PPILNVLLDTKSYANLYPFNICISLISGDKNFIMINILILISIIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            13602..14318
                     /locus_tag="PC545_001718"
     CDS             13602..14318
                     /locus_tag="PC545_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456489.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYFLMFDLLLAFITPFMFFFASTMVILGEIDDSISRYLIVTPLGK
                     TGYLISRFGIPGILAFIITMILLIFFSLTKISLLLNLSISLLVLLQGVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKAQYILFFLPTFWISKAFKEDNYVYMFISI
                     VISLIWILLLFKKFSKKIVK"
     gene            14415..14678
                     /locus_tag="PC545_001719"
     CDS             14415..14678
                     /locus_tag="PC545_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456490.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            14650..15105
                     /gene="def"
                     /locus_tag="PC545_001720"
     CDS             14650..15105
                     /gene="def"
                     /locus_tag="PC545_001720"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2456491.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIEQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            15157..15618
                     /locus_tag="PC545_001721"
     CDS             15157..15618
                     /locus_tag="PC545_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDF2456492.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHLLVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTSILKWKNRNFANDISNEHSLNLENL
                     YDDWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWSGNIMF"
     gene            15668..16570
                     /locus_tag="PC545_001722"
     CDS             15668..16570
                     /locus_tag="PC545_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2456493.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSMRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINSLFRHAKECNVHYVLP
                     GTLYLRGITRGVFFDFVKKEFPELFDKLSILYSAGSLNREYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            16940..17752
                     /locus_tag="PC545_001723"
     CDS             16940..17752
                     /locus_tag="PC545_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2456494.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPANYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLLEVENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            18800..19561
                     /locus_tag="PC545_001724"
     CDS             18800..19561
                     /locus_tag="PC545_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HDF2456495.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQHINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWNGKRVFIIATMALFSGDGSGVLSRILKKY
                     GAITVGGLHVKMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKHGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNMYTDKLKINTEECIVCGKCVNLCPMKNLVIKNCMIVAN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            19626..20408
                     /locus_tag="PC545_001725"
     CDS             19626..20408
                     /locus_tag="PC545_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2456496.1"
                     /translation="MEYYIVDSFATKLFKGNPAGVCVLDRRIPLELMQKIAEENNLPE
                     TAFVVKGKGNYELRWFTPKAEIDLCGHATLAAAYVISNFIDVNVKRIDFFTQSGKLEV
                     TRNGNLYEMIFPEIMPIEIELSPQQANLIGCVPSAVYSSRDLILLLNSEQEVINYKPN
                     YAQLRKLTDWLGIIITAQGSNTDFVSRYFCPELDSEDPVTGSSHCNLIPYWSEKLGKH
                     KMVAAQLSNRGGIIQCEVLKDNTVKISGEAVLFMQGTIKIDI"
     gene            20675..21094
                     /locus_tag="PC545_001726"
     CDS             20675..21094
                     /locus_tag="PC545_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HDF2456497.1"
                     /translation="MDKISGKLTVLFEEPFWVGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDNIGYKRMQRKVKKEQEKETIGTKAQNALKLQY
                     EERKQDRKNLAKNRKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(21197..21463)
                     /locus_tag="PC545_001727"
     CDS             complement(21197..21463)
                     /locus_tag="PC545_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDF2456498.1"
                     /translation="MKNFILIYFIVINSIAFFSMYIDKKRAIRNEWRIKEATLMSIAV
                     IGGSIGSIIGMYSFRHKTKHIKFTFGIPFILFLQFLLVYFYILK"
     gene            21690..22391
                     /locus_tag="PC545_001728"
     CDS             21690..22391
                     /locus_tag="PC545_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456499.1"
                     /translation="MKFIVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDMDFRNDDVYVRFSKKGDKFVPFGETDVELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            22473..23081
                     /locus_tag="PC545_001729"
     CDS             22473..23081
                     /locus_tag="PC545_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2456500.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSSE
                     QLKNIYLKNEEDSVDAVVIVGLHKSSKKLILIRQFRVPINGYIYELPAGLVDEGESID
                     ISVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDEFLEPDE
                     EIEALLISQEDAKEILQSNHKIDTKAFLILQMFVSLGAKLFE"
     gene            23405..23869
                     /locus_tag="PC545_001730"
     CDS             23405..23869
                     /locus_tag="PC545_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 domain-containing lipoprotein"
                     /protein_id="HDF2456501.1"
                     /translation="MKKLVIILISIISMVSLTACKKSNESKYNNLNNKESGQTTIEMD
                     LDKNYDTSDPFVNARLFCVSNDIDVLDTEISFKMDGDSGIVEIKDNKTDETLWSNTWH
                     GSVDNDTFTISLSNIQKEKEYAICFTGIKINHAVVKVTFESNLVKEKEKPSK"
     gene            24506..25522
                     /gene="gap"
                     /locus_tag="PC545_001731"
     CDS             24506..25522
                     /gene="gap"
                     /locus_tag="PC545_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2456502.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEKEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            25917..27086
                     /locus_tag="PC545_001732"
     CDS             25917..27086
                     /locus_tag="PC545_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456503.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGQEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLVG"
     gene            27466..31719
                     /locus_tag="PC545_001733"
     CDS             27466..31719
                     /locus_tag="PC545_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HDF2456504.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYEKLGDINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGANQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEDNIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINEVYDILPKNIKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEVNHAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANVQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMFERESKNIDVKNIKVNRIQYAKNNQITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAIYDAGFKDIPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKSSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEAFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            31868..32362
                     /locus_tag="PC545_001734"
     CDS             31868..32362
                     /locus_tag="PC545_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2456505.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDGFIVSIYIGDEKFNNLKNECIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKDIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            32478..32855
                     /locus_tag="PC545_001735"
     CDS             32478..32855
                     /locus_tag="PC545_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDF2456506.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTKAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            33061..>33473
                     /locus_tag="PC545_001736"
     CDS             33061..>33473
                     /locus_tag="PC545_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HDF2456507.1"
                     /translation="MKNLNLLINKLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMIDNENSYIRTRGLLLISANAQWDIDNKIEINIDSILSHIVDKKPSVSRMFIKSIPN
                     ITKYKENLINRIKMELSNADISIYNNNMKPLVEKD"
BASE COUNT        13491 a         3394 c         5608 g        10980 t
ORIGIN      
        1 tgttttatct atagtatcaa ctttgttagt atatttaatt gaacaattag tatatggatt
       61 tacagctcca ttaaattact tgatatttac tagtgatata aatacagata tttataaatt
      121 tgatataaat atgggactat cttctttaat tactattata atagctggat ttatatattg
      181 gtatagttct aaaaagttta atattaaggt catgaacttt gataaatata tagcaatttt
      241 gatgattcca ttgttgctta taatattatt tatgcaatca tttgaatata gttcaaatat
      301 aaatatagat acaagtcttg ggattgtaaa gctgctatta aatacttcta taactgaaga
      361 aattcaagct tttctttttt ctataattgg aactatatgt gtattttttt ctctatttac
      421 ctttaagaaa ctaattcaag ctctcgaaga tgacaaggag agggcaatta tgaatcaaca
      481 gattcatgct caaaaaaact atattgaaga agcgaaatca cgattatcac aaactatatc
      541 ctttagacat gattttaata atcatttagc tattgtcaat ggattgttga aaaaagacca
      601 aattttaaaa gcacaagatt atttaaataa gcttgaaaaa gtaacaagtg acctatcatt
      661 ttcttgtaat acacagaata ttgttattga tgctcttttc aacaataaat taagtatagc
      721 aaaacaatta ggcatagagg tagattgtga agtaattata ccccaaagca ctaacatgaa
      781 cgattttgac ctatgtatag tgtttgctaa tgctattgac aatgcaatta aagcttgtag
      841 agttgtagat aaacaaaata ggtatttaaa gctttccact aagttagagg gtggattttt
      901 tatgatagaa ataaaaaata gctataatcc aataaataat tatacaagtg gctcaggtat
      961 aggtctttta aatataaaag aaattgctaa aaaatatcaa ggggctataa gtatagataa
     1021 gacatcagat tattttcaaa taaatgtatt gctggttatt tcattacatt gcgctagtat
     1081 ttgataacaa tttcattgaa aatagtattt gtaagctata ttatgtactt gaatagagaa
     1141 agtttgatat atagaaatga ggaggttact aaaatgaaag ttggaaatat aaatggtcaa
     1201 gcaccaatta caaatggttt agatagttgt caaagtgagg tacaaggctt agaacaaaag
     1261 aaaaaatcat tacaggatga aatagagaaa gtaagaagtg ataaaagtat tgatggtgaa
     1321 acagccaaat taaaaataga agctttaaaa aaagaaatgg aagagattga aaataaaatt
     1381 caagagttaa aaggaaataa agcaaaaaaa acaaataaag aagaaaataa tgtttcagaa
     1441 gtaaataata atctattaga gagaaaaaaa gaaccttcta ataatgaaaa tgtaaaacaa
     1501 ttatatgatg tttttgaaag aagcaatgta gacgaacaat gggataatac ttatcgtgtt
     1561 ctccaagatg ataaaaactt aaaagtaaaa tttaatagac ctatatagtt tatttataaa
     1621 aatagttaat attttaatta tcaccacttt ttagaagaat acacacttta ttttaaagac
     1681 ccacatattt atttacagtt agcacattta tatgtggttt tataactatt atttacaagt
     1741 ctatattgat aacttttaca tagtatataa tgtttaaata cttattcaaa tttatctata
     1801 cataaacaaa aaaagagatt ttaaaatctc tttttagatt gttgacttat ggctttttga
     1861 tttaataaaa tcaagaagcc gtttgaattt tatctgttgg ctgataccct tctgatattg
     1921 aaacaccatt tttgtttcca aaatcgttta aaattaagaa cttattatga atgactattt
     1981 cattatgaac aataagcaga taacatgaat cttcaatcaa tgttttaaca gtagcattaa
     2041 atgcttgctt atacagttct atcgtttgtt tgaactgtcc attaaagtaa atatgagtca
     2101 ataacataac tatatctaca caaattaata taaatttttt atactgtaca ttatctaact
     2161 gttgattatc tgtattacaa catgattgaa tttaaaaagc catcaacaca tagaatgttg
     2221 atggcctgaa aatagattta aaatctcttt tttattatat attaattatt ttttaaattt
     2281 atattttttg catcagattt tctaatagct aagtcaaacc ctctaaatct aatcactata
     2341 gggtcattaa gaggagctat ctttttcact tcaacttcag tgccatctat tagtcctaag
     2401 gctagaagtc tcttcataag tttttcattt ccagctatat tgtctataat tcccttttgt
     2461 ccaagtttta agttgtaaac agtcattatt aacacctcct aatgaaaatc attatcaaac
     2521 tttatattta taatatataa caaaaagagc aaattgtcaa tatataacaa aaaataaaca
     2581 tactaagtta gtatgtttat aaaaaatagt atacatggga tactaatagc atctagcaaa
     2641 aaaccactta ctaatggtac aactaaaaag gcttttggag aatcaccata tttttgtgtc
     2701 aaagaactca tatttgccag agcgttcgga gtagctccta ttccatgtcc aatcaaacca
     2761 cttattatga tggcagaatc aaaatctttt ccaagtactc taaagcaaat aaatattgaa
     2821 aataggatta taaagattac ttgaaaagtt actattaaga gaataggagg taataatgta
     2881 gataattcaa ataaatctat attcataagt gataaagtta aaaaaattcc caatgaaagt
     2941 tcttttaaaa aatctataag tttaaaattt aattctagta tatttatctt atcatttaaa
     3001 tttctaaaaa taacagccga aaacatacaa gttacaacag agggtaatag tatattaaac
     3061 gttatataga agcatctact tataatttct cctgtattta tacaaataaa tatgagaaga
     3121 acctgttcca aaaatacaga tggtgttatt gtttcggaga agttattatt ttttatccta
     3181 ttcttatttc taaaaaattt acttccaaat ctctttagat ttctattata tctaggaatc
     3241 gacaagtcta tatttgatga tggttttaat ttatatttat ttattagaaa tttagctaca
     3301 ggacatccca atataccacc cataataagt cctattgttg cagcagataa tccaacccca
     3361 actgctcctt ctataccgag attttctatt gtaagtccaa atgcagcaga ataaccatga
     3421 ccaccttcca tagatacatt tccacacata agccctaaaa gaggttcaag ttttataatc
     3481 tttgatattg taatagctaa tatattttga caaaatgcca atactccaca caaaatccaa
     3541 tatctaaata atacactacc acctttcttt ataagattta tgcttatttc aagtcctata
     3601 gttgtaaaaa agaaggttat gaaatttggc attaaatttc cacttataga tatatctgct
     3661 atattaaaat atttaaaaaa taaatttaga aaacaaaata atagacctcc tattactggt
     3721 gcaggtatac agaaattatt taaaaagtta actttattct tcaataaatt accaattaaa
     3781 aagaagaata tagatagtgt caaggtttgc actatatcaa aacttaaggt aatcaaaata
     3841 agtcctcctt tgtaattcaa agttttaatt atataactta aattcttatg tattgaaatt
     3901 ttattatcaa atgtatatta ataaatgtat attaaaaaga atcagtagat ataattagat
     3961 agtcataaat taaattaaaa tattttaatg agatatgact tttttagaat taaaaaccgt
     4021 agtatattga tataatagaa ataagactta aaaatctgtt gatagtatta tttataagta
     4081 cagtaggagg ataaaatgtt tgaaaattta cctaagatag atttacattg ccacttagat
     4141 ggaagtgtta gagtagaaac catgcttgat atagctatta aagagaaaat agatttacct
     4201 tcaaataata tggatgaaat aaaaaaatta gcaaaggttt cttttaattg tacttcacta
     4261 gatgaatatt tagaaaaatt tgatttaccc ttaaaagtaa tgcaatctaa ggaaaattta
     4321 aaaagaatta cttttgaact tttggaagat gcaagtagag aaaatgtaaa atatattgaa
     4381 attagatttg caccattact tcacacccaa aaaggtatga gtgttaaaaa tataattgag
     4441 ggtataattg aaggaattag agaagctgaa agtatttatg atataaaagg caatttaata
     4501 ttaggttgta tgagaacaat gacctcaaaa gaggcattat tagtaattga agaagggaaa
     4561 tcatttgtaa ataaaggtgt tgtagcagta gacttgtgtg gtccagagaa agagggattt
     4621 tgtaaggaat ataaagatgt ttttaaatta gcaagagaat atggatataa agttacaata
     4681 catgcaggag aagctgcaag tggagaaaat gtattggatg ctataaatat tttaaaggcg
     4741 gataggattg gtcatggggt aaaaattaaa gaccataaaa aggcgtataa tttagttaaa
     4801 gataaaaaaa tattgcttga attatgtcca actagtaatg tacaaactaa gacagtagat
     4861 tcttatgaag cacatccatt ttatactttt tataaagata atttacatgt aagtataaat
     4921 actgataata gaactgtatc agatataaat ttaagttcag aactaaatgt tatatttgat
     4981 acttttaaac taggattaga agactataaa ataatttata gaaatgcagt agaagctagt
     5041 tttgcagata aagaaactaa agagtatttg aatagtttaa tttaatagat aaaaatattt
     5101 aaactttaaa ttaagctatt aaattgataa aaatacattg tattaggaga aaaaatgtga
     5161 aagattatga agatgttgtt tatattaaac tagtcaaaaa agaacttgaa gaatggaaga
     5221 aaaatatatt aaagaaacct tctatatttg acaaagcatc aaagggtata cagaataaat
     5281 ttaatggagt attgccagat aaatatcatg aaataattac ttcttctata aaagtaatga
     5341 caaaaaccgt actgtttgga tataaatatg ctacaaagcc accacttaga aatatgtcat
     5401 tagaagaaag agaaaaatta gttttagaaa aagctaaaac ttacaagact acagcaatga
     5461 ttgaaggggc aggaacaggg gcaggaggaa tcctcttagg tttatctgat tttcctttgc
     5521 ttttaggtat taaagtgaaa tttttatatg atgtagcttc aatatatgga tatgatgtta
     5581 aagattatag agaaaggatt tatatattaa atatattaca gttggctttt tctagtcaac
     5641 agcatataaa caaaactttt aaattgatga ataactggga tgaatatgta aatagtctac
     5701 cagaagacat caatgatttt gattggagaa catttcaaca agagtatagg gattatttag
     5761 atttagctaa gtttttacaa ctaatgcctg gaataggagc atttgtagga ttttatgtga
     5821 atggaaaatt aattgataaa ttatgtgaaa ctgctgtgtc tgcttataga ttgaggattc
     5881 gagagcttaa gttataagaa aaattataac ataaaaaatt agttatacaa tatatctata
     5941 tgtatctaat tttgcattga taaaaacaat tatggataaa aggtaaagca tgatagaatc
     6001 taatgtagag gtttgtattt taatggaaaa atttagtttt tattgcttaa gatacaaatt
     6061 gaattacgaa gagattgaat tatgaagaga agtaattggg aggaatattt tcatgacaaa
     6121 tttgccaaaa caatttgata gcttcaatga agctagacaa aaaggattta taaatgcaaa
     6181 ggaacttaag gaaagtggga aaaagatggt tggagttttc tgtacattta ctccagttga
     6241 aattccaatg gcagctggag caactgtagt aggtgtatgc ggcgttagtg aagagccaat
     6301 acctgatgca gaaagggtgc ttccaagaaa tctttgtcct ttaattaaat caagctatgg
     6361 acatgcaata acagatacct gtccttattt ctatttttct gatttgttga taggagaaac
     6421 cacttgtgat ggtaagaaaa aaatgtatga agaacttgca aaagttaagc caacatatgt
     6481 aatgcatttg ccaaatactt caaaaggaga atttgcctat aaactttgga aagatgaaat
     6541 gataagatta aaagaagagg ttgagaaatc tttaggtgta actataactg aagaagatat
     6601 aagaactgct ataaaagata aaaatgaaga aagagaactt ttaaaagagt tttatgcact
     6661 tggtaaatta caaccatctg cacttacagg ccttgagtta cataatgttt tatatcaagc
     6721 aggttttaaa tttgaccgtg cagaacttaa acattcttta agaaaagtta tagatgatat
     6781 gaaagaaaga tatgaaaaag gtgaatgtcc tgttcaaaaa gataagccaa gaatattgat
     6841 aacaggttct cctattggtg gaataagcga aaaaatagtt aaaactttag aagatgcagg
     6901 ggcatcagtc gttgcatatg aactttgtgg agctattaga tcaaacgatt tattggttga
     6961 tgaagaaata gaagatgtat atgatgcttt aacacaaaaa tatataaata taggttgttc
     7021 ttgtatgatg aataatgaca atcgtattga attattagat agaataatag atgaatataa
     7081 tgtagatgct gtaatagatg tagttttaca agcatgtcat acatttaata tagaaagtta
     7141 tagaataaga gaatttgtta caaaagaaaa aaataagcca tttatgtctc ttgaaactga
     7201 ttattcaaaa tcagatactg aacaattaag aactagattt gaagcgtttg tagagatgtt
     7261 gtaggaataa ataaaagtag gaggaactat gtttagtata ggagttgact cagggtcagt
     7321 ggcaacaaag ggtgtgctat ttgatggtga aaaaattatt aagaaaataa ttataccaac
     7381 tggatggagc cctaaaagta catctaaaca ggtctatgag ttgctaagta gtgaaattga
     7441 caaaaaagat ataaaaaagg tggtaggtac aggttatgga aggggagtaa tggattttgc
     7501 tgataagaaa gtaacagaaa taacttgtca tactagaggt atttactttt taaacaagaa
     7561 cataaggact attttagatg taggtggtca agatagtaaa gttataaatt tagatagaga
     7621 tggaaatgta tttaacttta taatgaatga taagtgtgct gcaggaactg gtcgattttt
     7681 agagattact tctaaccttt taggaagcga tattgaaagt atagatacat tagctaaagg
     7741 atatgaacca gttaatatat ctagtatgtg tacagttttt gctgagtctg aaattgtaag
     7801 tcttctagct caaaacattt ctactggaga ggtagcagca gggatactta aatctattgc
     7861 aaataaatcc acctcaatgt tagcaagagg tgaggtaata gatgaagttg catttactgg
     7921 agggcttgca aaaagtaaag aactagtaaa aatgatagaa gaaatactgg gtaaaaaaat
     7981 ttttattgca gaagatactc aaataatagg agctttagga gctgctgtaa taggatttag
     8041 ataaaaacat ataaaaagaa aatgagaagc tgaatttatt ataaataaat tcagtttttt
     8101 attttatttt atttatttat aaaggaaaac tttggtataa ttgatttaaa tgattgtata
     8161 tttgtaaata cttgatattt agtcatatat aacattataa ttttaaaaat tagctcaaaa
     8221 taagggggag aaagcgtgaa aaaatttact tcaaaaaaag taacaaggtt tgttgcgctg
     8281 tttttaatat ctattttagt aatgtcaaca attcctgtat cagctgacaa tagcaataca
     8341 ttgaatcaga taaaaaaagc agaatacagt gagacgtata aacaatatct aaatgatgct
     8401 aaaaatggat gtacagaaaa atataatggg attataccaa atccatatca gttggaaggt
     8461 actcaaatcc aaagtaaagg aagaacagct ccaacatctt atgaccctag aaaattaggt
     8521 cttatgacat caataaaaaa tcaagaggat ttaggaatct gttgggattt tgctgcaatg
     8581 gcaaccttag aaagtttctt aaagttaaat aattatggag actatgattt atcagaagaa
     8641 cacttaagat ggtgggcttc agatggtgaa tatggatggt ctgtaaatga tatgaatggt
     8701 gctttaaatt atgaagctat gggatattta acatcttggt caggtcctaa attagaaaaa
     8761 gatatcccat acaatggtag agtatctaaa gcacaaggag caaaaaaacc aactaatatg
     8821 aatacagcac ctactgtttt taatgtgaca gatgctgtgt gtgtaagcaa tgacataaat
     8881 tctacaaaaa atgctatatt acagtatgga gctgtaacat ctggttatta tgaagatatt
     8941 aaatatcaaa gtgatgacca aaattcatat tattgtccaa caaaaagttt taatacaaat
     9001 catgctattt caattgtagg ttgggatgat aactattcta aagataattt taatgcaaat
     9061 attagaccat caaaaaatgg tgcttggtta ataaaaaata gctggggaga ttataattct
     9121 gagggtggat atttctggat atcttatgaa gacaagacac ttatgtctga tatagataat
     9181 ttttcaatca aaggtggtaa aaaaccaaat gatgatgaga agatgtatca acatgactat
     9241 gcaagtattg tacctttaat ttcaaaaaag ataacagctg caaatgtatt tgatttcaat
     9301 agaggcgatg aaactctaaa atctgtaatg ttcttaactg aaagtatagg agctaagtat
     9361 gaagtatatt atgctccagt agtaaatggt atacctcaag aaaacaatat gaaaaaatta
     9421 aaagaaggta ctgcccaata ttcaggatac ataacagttc caattgattc ttttgatata
     9481 ccagaaggaa agggtgcaat tgtagtcagt atagagggga aaaatggaga gtcaactata
     9541 ggttctgagt caaatgtacc aggatatgat acatttaaag caaaagctaa tttaggtgaa
     9601 agttatatca tagataatac tggaaaattc tttgatataa atagagatag taacttctat
     9661 ccgtgtaatt ttactataaa agctgttaca gaaaagtctt ctggtgagag tatcccaaat
     9721 gagtctttaa ttggtagtga tagatatgaa actgctatca aagttagtca aaatggtttc
     9781 aattcttcag aaaatgtagt tttagtaaat ggcagttcaa tagtagatgc attggcagca
     9841 actcctttta catctgctat aaattcacca atattattga cacaaaaaga agctttaaat
     9901 tcaaaaacta aagctgagat acagagatta ggtgctaaga aagtatacct aataggtgga
     9961 gaaaattcta taagtaaaga aattgaacaa caattaaaaa gcttaaatat atcgatagag
    10021 agaatatcag gaagtgatag atacaagaca agtttattat tagctcaaaa gttaaatggt
    10081 ataaaaaatg tatctcaaat agcagttgta aatggagtga aaggattagc tgatgcaatt
    10141 agtgtaggcg ctgcagcagc agaaaataac ataccaatca tacttgcaaa tgaaaagagc
    10201 gaattgcaag gagctgatga atttctaaat tcattaaata tagagaaatc ttatattatt
    10261 ggaggaacag catccctatc aaacaatcta gaaagtaagt taaaaaatcc aactagatta
    10321 tctggtagta gtagagacga aactaattca aagataatag ataattttta taaaaaagat
    10381 actttaaaaa atgcatttgt agttaaaaat ggaatcaaga atcaaaatga tttaatagat
    10441 ggattggcag ttggtccttt aggagcaaaa actgggtcac cagtaatttt agttggtgat
    10501 aaattagctg atagccaaaa agaagttctt aaaaataaga ctttagaaaa agtaactcaa
    10561 gttggtggag gagcaaataa aaatgcattt aaagagttag taaaattaaa aagtgctaaa
    10621 tagagtactt aagtattaac atatattagt gtgtattagt aacttatctt aataaaagct
    10681 gaaaataatt acttcaaatt attgataaat aagataatca atgattgtaa agttttgttt
    10741 tcagctttaa atttatgtag tataagttta aaaacataaa aaagggttac tttatataga
    10801 aatatagaaa atcttacatt ttatatataa ataacccata tttaattaac tttttatagt
    10861 gttctaatag atattattga acttaatggt aaatataatg tgtaagtttc atttaaagtt
    10921 ggattagatt catctatata tcttgagcaa agtgtaattg ataagctatc tgcttctata
    10981 atttcaccat atagaggaca actgctaaag taagaatgta cttcaacata tttatttaag
    11041 ttttcttgaa cataatcgta aaaacggttt tttgtagtag tagccataaa atcaactcct
    11101 ttatttttta ttaaagttga aacttttaaa aagataaata tgattacttc tattagaaac
    11161 taagttaaaa aataagcaaa tagatatgaa aattagaata atatattatt tatatcttaa
    11221 ttataacact agataataaa aaatgctata taaaattcag aattttcgaa agtttttaag
    11281 cttattttta aatatactaa taatactatt gacattagat agaaaaagta ttatattgac
    11341 tatataaatt attttagtca aactggaggg aaaagatgcc taaatcatat tctgataaag
    11401 aaagagagta tataataaaa agacttaaag aagaagcaag attgtgtatg gagcaatatg
    11461 gtattagaaa aactactgtt gatgagcttg taaaacgggt aaaaatacca aaaggaactt
    11521 tttatctatt ttttcaatca aaagaattac tcttttttga ggtattaaga gatatacatg
    11581 actctataca aaaagaaatc ttatatgaga taaatagagt agatgaaagt attacatgtg
    11641 aacaattgac agatatattt atgaaattct ataggatggt tgatagtact tcaattttaa
    11701 atctcatgat aaatggagaa tttgagatat tggtgagaaa gttaccagat tctataattg
    11761 aagaatactt tagacatgat gactttgaaa ttggagagat tatatcttgc ataccaaatg
    11821 caaaaaataa agacatagaa agctttagtg gagcatttag agcagttttt cttaccatgc
    11881 tatataagcg cgaggttgga agtaattgtt ttgaagatgc tttaagatta atgataaacg
    11941 gattagttat acaacttatg gaatagaatt taaattttgt ttgctaataa cttttattaa
    12001 aaattattaa caaacaaaaa aattatattt tattgaccat atagtcaaaa tagtcaaaaa
    12061 tttgaaaggg tgatattgaa tgattaaagt tgatgattta tcatttagtt atacagatag
    12121 agatttttta caaaatatta actttgaagt aggaaaaggg gaaatactag gatttttagg
    12181 accatcaggt gcaggaaaat ctacattaca aaagattcta attggaatga taactaatta
    12241 tggaggaagt gtcattgtaa atggagtaga gagtaaaaga cactcaaata aattttatga
    12301 aaatatagga gttgattttg aatttcctag tttatatgaa aagttgactg caatagaaaa
    12361 tttaaaatat tttgggtctc tatattctaa aaaactacta tctattgatg agttattaaa
    12421 atcagtagga ctagaaaatg aatcaaataa aagggtatct gagtattcaa aaggtatgaa
    12481 atctcgatta aattttataa aagctttatt acataatcca gatattttat ttttagatga
    12541 accaacaagt ggtcttgacc catcaaatag caaggttatg aaagatataa ttttatcaga
    12601 aaaatcgaaa ggtaagacta ttattttaac aactcataac atgttagatg caacagagct
    12661 ttgtgacaga gtagcgttca ttgtaaatgg taaaatatct gctttagata caccacataa
    12721 tcttattatg tcaaaaggag ctattaaagt tagatatact tactttgata atggagaaaa
    12781 aacttctgaa tgttttctga acaatactgc taatgataaa aatttgaata tgcttattga
    12841 gaaaaataaa cttttatcaa tacatagtag tgaaccaaca ttaaatgata tttttattga
    12901 gataacaggg aggaatttac aatgaggcta aaaaatctta ttttaggaga tataaagttt
    12961 caatttaagt atggttttta ttttttatat ctttttttaa gtattatata tatttgtatt
    13021 atcaatgtat ttccaacttt tatgagagaa aaaatagcta taattatgat ttattctgac
    13081 ccagcagcaa tgggattgtt ttttatggga gctattgtac ttcttgagaa aagtcaaagg
    13141 gttttaaatt ccttagctgt atctccagta aaagtatctg agtatatttt atcaaaggta
    13201 atttcattag gagtaatatc tagtattgta gctatgttta ttgctattac attaaattta
    13261 gataatatta taatatcaac tataggtacc tttttttctt ctattatatt ttctttgctt
    13321 ggattgataa ttgcaagtaa agcatctagt ttgaatcagt ttattgtttt aagtattcct
    13381 attgagatta tatgctttat tccaccaata ctgaatgtac ttcttgacac aaaatcatat
    13441 gcaaatttgt acccttttaa tatatgtatt tctcttatta gtggagataa aaattttata
    13501 atgattaata ttttaatttt aatatcaatt ataataataa tttattttat aacttattat
    13561 tttatttgta gcagttggaa aaaggtaggt ggagtaaaac tatgataaag attataaatg
    13621 cgtttaaaca agagtttgaa caaattaaga gagatgctat gctttttata gtatgtgttt
    13681 ctccaatatt atgtggagta tttataaaat ttggaatacc attaatacag aatatatcac
    13741 ttaacaagtt ttattatcaa ttaaatttag agccgtattt cttgatgttt gatttacttt
    13801 tagcttttat tacacctttt atgttctttt ttgcttctac aatggtcata ttgggagaaa
    13861 ttgatgacag catttcaaga tatttaatag taactccgct tggtaaaact ggttatctta
    13921 tttcacgatt tggaatacca ggtatcttag catttattat tactatgatt ctacttatct
    13981 ttttttctct aactaaaatt tctctcttgt taaacttatc aatttcttta ttagttttat
    14041 tacaaggagt tattatatca atattagtga tttctttatc atcaaataaa ctagaaggaa
    14101 tggtaattac gaaattctca ggtgttttta tgatgggtat tttagcacca ttttttattt
    14161 taaataaagc acaatatatt ctgttcttct taccaacttt ttggatatcg aaggcattta
    14221 aggaagataa ttatgtctat atgtttattt caattgttat atcattaata tggattttat
    14281 tattatttaa aaagtttagt aaaaaaatag ttaaataaga aaatttaata tataaatgta
    14341 aggttgactt gacagtaatg ttgaaaaatg ttacatttaa gatgtaaagt taaccttaca
    14401 tgaatggagc aaatatgaaa aataaaataa agatattaag agaaaaatta ggattgacac
    14461 aagaacaact agggagatta gttggaacat ctagacaggc tataaatgct atagaaactg
    14521 gtaaaaatga accttcaata tggttggcat atgatatttc aagaattttt aatgaaccaa
    14581 ttgaaagtgt atttttattt gaagaaagtg aaagaaaatc aagagcacag ataagtaggg
    14641 gagaatatta tggctataag aaagataaga acatttgatg atgaaatttt aagaaaaaag
    14701 agtaagtatg tagaaaacgt agataataag ataagagaaa tattaaatga catggcagag
    14761 accatgtaca atactccaaa tggaggagga ttagctgcat gccaagtagg agtattaaag
    14821 cgattagttg ttatagattt aggagaagga cttattaagc ttgttaatcc tgaaattatt
    14881 gaacaagaag gggaacaaat tgttgttgaa ggatgtttaa gttttcctga agtatggggt
    14941 aaattaaaaa gaccaaaaaa agttacagtg caagctctaa acgaatacgg tgagaaaata
    15001 gaaattaaag gttcaggttt tatggcaaaa tgtctttgtc atgagataga ccatttaaat
    15061 ggtatagttt ttacagataa aattattgaa catgtaaaat tgtaagatag attggttatt
    15121 gaatttataa aatattttat agtaaggagt taaattatga gattatggca taaggattta
    15181 attgatgtat tacctaaaaa tcaattagta agtcaatgga gagagctact tgctataaaa
    15241 ggttctatag ataaaaaagg tactccaaac catctattag tgaataaagt attgaattat
    15301 agtatagatg aatttaaatt ttatacaaaa attgtacatg atgaaatgtt aaaaagaaat
    15361 tataaaccta atgaacttaa atatacaagt atattaaaat ggaaaaatag aaattttgca
    15421 aatgatatat ctaatgaaca tagtttaaat ttagaaaatt tatatgatga ttggcataat
    15481 aaaatgtatt taaagcaatg cttatataat ttagaagaaa aagcaacatg tggaggaata
    15541 ccaataaatg aatggaatat attactttgt aaatatagta aagattatga attgtggtct
    15601 ggaaatatca tgttttaatt actatagatt aacattatag tattattgta tataaagagg
    15661 tgattatttg tataataaaa taaaatatat tccaataaat tgtgaaatag cttgtaataa
    15721 attaaaaagg agtatgccat ataaatggga tttaaatatt tatcgtggat gtgagcatgg
    15781 atgtaaatat tgttatgcta tttactctca taagtacatc aactccaata attattttga
    15841 agagatttat gtaaaaacaa atatagtaga aaaacttgag agacaactta aaagcagtaa
    15901 atggaagaaa gaagtaatca atataggagg agttacagat agttatcaac caatagaagc
    15961 tgactataaa attatgccag aaatattaaa cctgctaata aaatacaaga caccagctat
    16021 tatttctaca aagtcagatt taattttaag agattacgat ttaattgata agctatctag
    16081 gattacatat atcaatgtag catctacaat taccacagta aatgaagata cacaaaggct
    16141 tattgagcca aatggtgtgg attcaatgag acgatttaaa atgttaaaag aatttagaaa
    16201 aactaacgca tcagttggtc tacatattat gccaattata ccatatatta cagatgattt
    16261 tgataatata aattcgctat ttagacatgc aaaagaatgt aatgtacatt atgttttacc
    16321 tgggacttta tacttaaggg gtataactag aggtgttttt tttgactttg ttaaaaaaga
    16381 gtttcctgaa ttatttgata aattatcaat actatatagt gcaggttcac ttaataggga
    16441 gtataaaaat caattgtata aaatggttaa tgaacttaga tataagtatt ctctatcaag
    16501 cagttactct aaaataatga aggaaaagtt aaaaaactca ggagatgtac agatatcttt
    16561 ttttgattag ttttatataa aaaaattttt tttactatga tgattaaaat ttattaaaat
    16621 taaaatttat tgacaaatat aaaatttaaa tatataatta attcataaaa taaaaatatg
    16681 aaaggatgat gttgttcggt gatatttgat atcataaaat cgttgatata agtattgctg
    16741 gaaaagagtg aataaaatca aatttaataa gcaaacaaag taaagctata gttttattgt
    16801 tgtgcttaaa aatatttggt tctattttac tcttatttaa actcatcttt ttgaagttaa
    16861 accacagagt aaactctgtg gtttttttat tttaaaaata aattttaagg agtggtatgt
    16921 tttgttaaac aaattatgta tgtatttaga aagaccagag ctttataaac aaagtgaaat
    16981 taatttttgg gatgatgagt atatttcaaa gcaattatta aaagcacact tagacactaa
    17041 ttttgaagga gcaagtagaa attttaactt tatcgaggat tctgtaaatt ggatagttac
    17101 agttgcaaat ccagctaatt atcctaaatt acttgattta ggttgtggtc caggtttata
    17161 tgcagagaaa tttgcacaaa aaggttataa ggtaactgga attgatttct caaaacgttc
    17221 tattaactat gcacaaaata gaaataagga gactaattta aacataaatt acttgttcca
    17281 aagttattta aatatgaact ataatgagga atttgatttg gctactttaa tttattgtga
    17341 ttatggagct ttgtcaactg aaaatagaag gttattaatg gaaaaaattt atgatagttt
    17401 aaaacctgga ggaaaattaa tattagatgt atttacaata aataaataca ataattttga
    17461 ggaaattaaa tattgggaaa taaatgaaga tggaggattt tggagtaatg aaaaatatat
    17521 gtgtctacaa gataattgta aatacaatga ttataataca ttagaacaaa ctcttgtaat
    17581 aaccgaaaaa gatgaaaatg tattttatgt ttggaatcac tatttttcaa aagaaagttt
    17641 cttattagaa gttgaaaata ttggatttaa gtctgttgag ttttttagta atgttaaggg
    17701 tgaagcatat tctgacgata gtatgactat gggattagtt ttacaaaaat aataattttt
    17761 ttaaaatgaa taaataaaaa cttgaaacct ttaacaataa aataacataa taaaagctta
    17821 catattgaaa aatggaaact tttatgttat ttttagtaca ttacaaattt gcttatataa
    17881 aaatcaatac ttgaattatt atttttatgt gaaatttatc tatagtatat ttttataaac
    17941 aagtaatgtt attaaaatta agtatgtagt taaaagtgta tagcaaaaag gctgttatat
    18001 taatgttaga ggttcaggga aaatatataa agcaaagtat ttacagataa tgtagattgc
    18061 acagctataa gttagataat agaattatgt aatcaagaat ttacaacaaa gtataagata
    18121 aggataatgc taaattgcca tacaggtgct agatgtgtaa atggatttaa ttaatactat
    18181 tgataaagtg caacaagcac ataaaccaat tgatgagatt attaaaacta taagagatat
    18241 agttgacata attaaaatat tggaacttgt ttataatttt aaatcaaatc aataatgatt
    18301 aagattaaaa taatattaag gcatcaatgt tataagagta caatataagg agtactatat
    18361 agtactcctt acttatacat attaggcaat aggaaatttt ttcatagtta aattttcaag
    18421 tattttttag gaaactttat aattgagaat taagggtata gtatgtgatg aacttactga
    18481 atttataatt aggttatata atggaataat catcaatagt tttactttag ggaaaatata
    18541 tgcttaagga agtatagctt actaatatta ttaaaatgta tatttaaaat gataaaaaaa
    18601 gaattagaca agtatttagt aaattgacta tgcaaaattc tatcaagcag gaaccctatg
    18661 actttagtca tgggaggttc agtaggtaac ttgttgaatt aagtttatat tatttaaata
    18721 aactacttaa tttagataat ataaaaagtc agaattttaa gaatagagct atataatctt
    18781 agctattagg agaattacta tgataggaat atattttagt ggaacaggaa attcaagata
    18841 ctgtgtagaa aaatttttac aagaatatga tataacagca aattcttatt ctattgaaga
    18901 taatgaactt ttacagcaca tcaataatca tgagaatatt atttttagtt atccggtgca
    18961 atatagtaat gtgccaaaaa tattgaaaga ttttataata aatcattcaa ctttgtggaa
    19021 tggtaaacgt gtttttatta ttgcaactat ggctctattt agtggagatg gctctggtgt
    19081 gttatctaga atattaaaaa aatacggtgc tataactgtt ggtggattac atgtgaagat
    19141 gccagatagt ataggagatg aaaaggtatt aaaacattct ttagagtata atagaaagct
    19201 tgtaataaaa gcagaaaaga aagtaactaa agctgctaaa aaactgaaac atgggtcacc
    19261 accaaaagaa gggttaggtt ttttgagtca tattgtaggg ttatttggac aacgattgta
    19321 ttttatcaac aaaacaaata tgtatacaga taaactaaaa attaatactg aggaatgtat
    19381 tgtatgtggt aaatgtgtta atttatgtcc aatgaagaat ctagtaatta aaaattgtat
    19441 gattgttgca aatgggcaat gtacaatgtg ttatcgttgt gtaaataatt gtgctaaaaa
    19501 agcaattaca ttgttaggta aaaaagttgt tgaacagagt aatattaaaa aatatatgta
    19561 agaaatttaa agttttattg aatgtaaatt gatgtatatt ttatgttaga aagagaggtt
    19621 aaataatgga atactacata gttgattcat ttgcaactaa actatttaaa ggaaatcctg
    19681 ctggagtttg tgttcttgac agaagaatac ctttagagtt aatgcagaaa atagcagaag
    19741 aaaacaattt accagaaaca gcatttgtag ttaaaggtaa aggaaattat gaattacgat
    19801 ggtttactcc taaagcagaa attgatttat gtggtcatgc aactttagca gcagcttatg
    19861 taatatcaaa ttttattgat gttaatgtaa aaaggataga tttctttact caaagtggaa
    19921 agcttgaagt tacacgaaat ggaaatctat atgaaatgat atttccagaa ataatgccta
    19981 ttgaaataga gttatcacca caacaagcta atttgatagg ctgtgtacca agtgctgttt
    20041 attcttcaag agatttaatc ttattgctaa acagtgaaca agaagttata aattataaac
    20101 ctaattacgc acaattgcgt aaattgacag actggttagg tataataatc acagcacaag
    20161 gaagtaatac agattttgta tcaagatatt tttgtccaga attagattca gaagaccctg
    20221 ttactggttc atcacattgt aatcttattc catactggtc tgaaaagttg ggaaaacaca
    20281 aaatggtagc tgctcaactt tctaatcgtg gtggtataat tcagtgtgaa gtgttgaagg
    20341 ataatactgt gaaaatttct ggtgaagcag tcttgtttat gcagggaaca ataaagattg
    20401 atatttaaat aagtataata actaatttat tatgaaaaat aattaatttt cttagtgtta
    20461 acaataataa agtagagtta aaaaacttga tttcacttaa atcaatatga tagatgttga
    20521 tactaagatt gacaagcatg attaaaaaag ataatattta atctataaag ataaaaacac
    20581 agtctggttg gtagtccaga cgcagcaata gctgtcagta acctgcctcc ttggttgtcc
    20641 gttctttaga aagtaattta aggaggaact agcaatggac aaaattagtg gaaaattaac
    20701 agtcttattt gaagaaccat tttgggtagg tatatttgaa agacaagatg gtaaaaaata
    20761 tgaagcatgt agggtagttt ttggagcaga acctaaagaa gtagaagtgt atgaatttat
    20821 acttgaaaga tttttctcat tagattttgg tagcattaaa ttagaaaaaa atgtgaccaa
    20881 agacaacatt ggatacaaaa gaatgcagag aaaagtaaaa aaggaacaag aaaaagaaac
    20941 tatagggact aaggctcaaa atgctcttaa gcttcaatat gaagaaagaa aacaggatag
    21001 aaaaaaccta gctaaaaata gaaaagaaga agaaaaagaa agactattta atttgaaaca
    21061 agaaaagaga aaagctaaac ataagggtca ttaattttaa ttaaatatct ttttaataat
    21121 tgctagaaaa aggtgtataa aatttttaat tttatacacc ttaatttatt gaaaagaata
    21181 aggttttata tttcaattac tttaaaatat aaaaatatac taataaaaat tgtaaaaata
    21241 gtataaatgg tataccaaat gtaaatttaa tgtgtttagt tttatgtcta aaagaataca
    21301 ttccaattat agaacctata ctacctccaa ttactgctat agacataaga gtagcttctt
    21361 ttattctcca ttcgtttcta attgctcttt tcttatctat gtacattgaa aagaaagcaa
    21421 tagaattaat cactataaaa tatattagaa taaaattttt cacagtcttg cctccataaa
    21481 aatatattat acattataaa acaagatatt tgttttggaa agttaaattt gtaattagta
    21541 catataaaat atttgataag aacaaatata atattaattg gaaaatttaa aaaaattaaa
    21601 caattagcaa atagggggta tatttataag acacaaagtg ataaaatcta tttagaaaat
    21661 aatgtaaaat aacaggagga taaagcaaaa tgaaatttat agtagaaaaa gaagtttttg
    21721 ataaattaga aaatgtatgc tttggtgtag tagttgcaaa aggaatagat aatactaaag
    21781 aaatagaaag aataagcaac cttttagata taagtataga tagagtagaa gattacttca
    21841 aagataaaaa agtaaaggaa tcagaagaaa ttataccata tagagaggca tttagaagtc
    21901 taggcatgaa cccaaataaa tttatgagtt cgatagaagc aatgacaaca agggttgcta
    21961 agaataagaa acttcctcat ataaatccta ttgtagattt aggaaactct atttctttaa
    22021 aatatttact tccaatgggt gctcatgata tggattttag aaatgatgat gtatatgtta
    22081 gattttctaa aaaaggggat aaatttgtac catttggaga aactgatgtt gaattaatgg
    22141 aagaaggaga attaatttat tcagttggag acatggtaaa aactagaaga tggatatgga
    22201 gacaaggaga agaaggaaaa attactaata gctcaaaaaa tatatttttt cctatagatg
    22261 gttttactga tgctaattta gataaggtaa tgagtgcaag agaagaatta gcaaaattgt
    22321 taaaggaaat atttaattgt gaaattaaag taggttttgt agataaagat aatcctgaaa
    22381 tggaaatata ataagttgtt gaatacttgt taagatatta tatttactta atgatgttaa
    22441 aaaggtatta aaaaagaaag gtcgtagtaa atttgaaaaa aactataata aagaatgtaa
    22501 atccaatgat tgaaaataag tttataggac tttttgaaat agagtataaa aacaaattag
    22561 atgaagataa ggtttggatg gtagcatcaa gaaaaagtag tgagcaatta aaaaatattt
    22621 atttgaagaa tgaagaagat agtgtagatg cagttgtaat agttgggctt cataaaagta
    22681 gtaaaaaatt aattttaata agacaattta gagtacctat taatggatat atatacgagt
    22741 taccagcagg tcttgttgat gaaggagagt ctatagatat ttccgttgaa agagaactta
    22801 gagaagaaac aggacttact cttttagaaa ttaaaaaaaa taaaagtatt gataaagttt
    22861 atttatctcc tggaatgagt gatgaatctg ttgcctttgt ttattgtata tgtgatggtg
    22921 atataacaga tgaattttta gaaccagatg aagagattga agcgctttta atttcgcaag
    22981 aagatgcaaa agagatttta caaagtaatc ataagataga cacaaaagca tttttgattt
    23041 tacaaatgtt tgtgagttta ggagctaaat tatttgaata gatatataga aaagaggtta
    23101 tatagaagag taaaataata aagtttattt tacttgtgta aattataatc tcttttttta
    23161 tacaataaag tataattttg tgtattttta aataagtgat ttgactctta aattttcata
    23221 atatgttata aaccaattta agatactaca ataattaaaa ttccaaaatt atttaacata
    23281 tttgcaatta aaatgagaaa atgtaaaggc tgaatgattg aaaaatttga tgtaaaatag
    23341 tagcttagtt taatactata gtttgtaggg ttaaaaatta gaaattgtaa gggagataaa
    23401 ttctatgaaa aaattagtta ttattttaat tagtatcatt agtatggtta gcttgacagc
    23461 ttgtaaaaaa agcaatgaaa gtaaatataa taatttaaac aacaaagaat ccggacaaac
    23521 aaccattgaa atggacttag ataaaaatta tgatacttca gacccatttg ttaatgcacg
    23581 tttgttttgt gtgtctaatg atatagatgt tttagacaca gagatttctt ttaaaatgga
    23641 tggagatagt ggtattgtgg aaataaagga caataaaaca gatgaaactt tatggagtaa
    23701 tacatggcat ggaagcgttg ataatgatac attcacaatt tctctttcta atatacaaaa
    23761 agagaaagag tatgctatat gctttactgg aattaaaata aatcatgcag ttgtaaaggt
    23821 tacttttgaa agcaatttag ttaaggagaa agaaaaaccc tcaaaataaa ttccagcttt
    23881 tctataactt gatattcagt acataaaagc agttgatatt tatgattgac tgctttttct
    23941 tatcaaaaag gataggttgt gcatttttac ttgaaatagt ttatgcacat caaaagttgg
    24001 tgagcatgat attggaaatg gtaagcatga aatcttgata atacaaggaa ataaatttaa
    24061 gttttcaatt aagtatattt ccataattta gttaacaata agctaattat tatttatatt
    24121 ttgatatata aataataatt gtagcaaatt ttaaaaaatc atatattttt tgaactaaat
    24181 aaaaatttat ttttattatt ttaaattaga ggagattctt gaagatatat tatttttaaa
    24241 aataagtata gtttaaaaat tcaaaaaaaa gatggcaaaa tatgtcccaa gggacattga
    24301 gtaaaataaa tgatttcagc ttatttacac taaaaagtac atactaaatg aggggtaaaa
    24361 taaagaaaaa tgatatacta attgtctttt gataataaaa aaacatggaa tttgattatt
    24421 taatcaagaa aaagtattga acaaatgatt tatttagtgt acaataatca tgtaaaaaca
    24481 tagtaatgga gggagacatt tcataatgaa aattaaggta ggaatgaacg gatttggaag
    24541 aataggaaga gctgtattaa gaatagctca agaagaatta ggagataata tagaaattgt
    24601 agccataaat gcaagagcaa caacagaatc tttggcacat ttatttacat atgattcatg
    24661 ctatggaact tttagaggtg aagtagaggc aaaggatgaa gacactctag tagtaaactc
    24721 taaagagata aaaatattaa gatataatga cccagaagaa ttaccttgga aagaattggg
    24781 ggttgatata gtaatagaat caacaggatt atttacacaa agagaaaagg cagaaaaaca
    24841 tattaaagca ggagcaaaaa aagtaattat aacagctcca ggaaaaaatg aagatataac
    24901 tatagttatc ggagttaatg agaaagaata tgataatgaa aagcacaata taatttcaaa
    24961 tgcatcttgt actacaaatt gtctagctcc ttttgctaaa gtattggacg aaaaatttgg
    25021 tatagtaaaa gggttaatga ctacagtaca ttcatatact aacgatcaaa gaatattaga
    25081 taaaagccat aaagatttga gaagagcaag agcagcggct gaatctataa tacctaccac
    25141 tacaggagca gcaaaagctg tgtcaagagt actaccacaa ttagaaggaa aattaaatgg
    25201 tttttcactt agagtaccaa caccaacagt ttcacttgtt gatttagtgt gcgaacttaa
    25261 agaaaacgtt acatctgagc aagttaactc tgtgttaaaa gatgctgctg aaggagaatt
    25321 aaaaggtgtt ttaggatatt gtgacaagcc acttgtttct atagattata aaggagattc
    25381 aagatcatct attatagacg ctctatccac aatggttata gaagacaata tggttaaggt
    25441 agtttcttgg tatgataatg aatggggata ttcttcaaga actgttgact tagtaaaata
    25501 tgtagctgaa aaattaaaat aatatatatt tcttaaagca aattttaaaa aaatttttta
    25561 aaaaaattat ataaaactaa aaaaaaagat tatgaaaaat attgaaggaa aaattttttc
    25621 ataatctttt ttctttcatg caaaaagata caagttacaa cttatatttg catattaggt
    25681 cgttagagaa gtatatatat atttagtaag gaatcatact ttttataaaa tgaaagataa
    25741 gatgtcgaaa acttgcataa atctgaaaaa tttttattat taaaatactg atttccattt
    25801 taaaaatgat aaaaaaatga caaataaagt tgcaaatagt aatacttata agtataatta
    25861 aaaaagagaa aaggttttgt taaactttat aacaaaaaaa gaagggatga ttcaaaatgg
    25921 agaagttaac agacaggaga caggttaagg cagtaagcat ggaaaccttc attttcctaa
    25981 tattactagt agttggtttt gggtatgttg gtagtattat gggagcagga atgatgttca
    26041 aagtaataat gagtacagca catgctttac tattggagac agttttctta attatggcta
    26101 tggcagttct tgcaggcgca ttaagcgcac tactttcaga gtttggagta atagcgttaa
    26161 ttaataaaat tttcgctgta tttatgaagc ctttatatgg actacctggt gctagtatag
    26221 caggagctat aacaacttat ttatcagata atccagctat tatacctttt gctaaagata
    26281 aaacattcac acagtatttt aaacaatacc aagttccagc attatgtaac ttaggaacag
    26341 cctttggtat gggacttatc ttaacaacat ttatgatttc tcaaggtaca gaatatgtac
    26401 ttccagcatt aattggtaat ttaggtgcaa ttataggtag tgtcataagt gttagaataa
    26461 tgttgacatt tactaagaag ttttataaat acaatccgga agaagataaa gcaacaggaa
    26521 cattagagaa aaaggatgaa tttagagaaa tcagagaagg aaatgtattt caaagagctt
    26581 tagatgcaat tcttgaaggt ggtaaaatgg gggtagatat gggaatggct ataattcctg
    26641 gagtcctagt agtatgtaca ttggtgatgt tattaacttt tggaccatct acagatcctg
    26701 ttacaggtca agaagtttat acaggagctg catatgaagg aataaaatta cttccagtta
    26761 ttggagataa acttggattt atattagaac cattatttgg atttacatca ccagaggcaa
    26821 tagcattccc tataacttca ttgggagctg ttggcgcagc aatgtctcta gttccagagt
    26881 ttataaagtc aggagctata actcctaatg atatagcagt atttacagct atgggtatgt
    26941 gttggagtgg atatttaagt acacatattg gtatgatgga tgcattaaat gctagacagt
    27001 tggcaggtaa agctattctt tctcatacaa taggtggttt atgtgcagga gcagctgctc
    27061 actttatatt tacactcgta ggataaatgt aatcagctaa gataaaaaat aatatacaaa
    27121 aaatggttgt tgatatgtaa taagaattta catatcaaca accatttttt tctgactaaa
    27181 gataatttat aatacataaa cattaagtgg atattagagt taagttatat ttatgtaaaa
    27241 agtaacaaaa atctgtagaa atttatgaca aaatttagta cactaactat atgttcttat
    27301 ttaaaattta aggttagaat taggaattac ctagtagagt aagctttgat taataaaaga
    27361 atcataaaat aaaaatatta gtcataaaga ttataaataa cttacttaaa ataaagccat
    27421 agaacttaca aaaattaaaa aagcatataa atatggaggg gtacaatgga tgaattattt
    27481 catataggaa ttgatgtagg ttctacaacg gtgaaggttg tggtacttaa taatagtaat
    27541 aatatagttc ataaagaata tagaagacat tattctgatg taaaaaaatc agttaaagaa
    27601 gtactaaatg gtatatatga aaaattggga gatataaata ctaccattat aataactggt
    27661 tcaggtggaa ttggaatatc aaaaaaatta ggtgtaaaat ttgttcagga agttatatct
    27721 agtacaaaat caatagagta ttttcatcct gaaactgatg ttgtcataga acttgggggt
    27781 gaagatgcaa agataacata tctaagtgga ggaatagacc aaagaatgaa tggaatatgt
    27841 gcaggtggca ctggagcatt catagaccag atggcatcac ttttaaagac agatgccagt
    27901 ggattaaatg aacttgctaa agggtataat gttatatatc ccatagcttc aagatgtggt
    27961 gtttttgcta aaacagatat tcaacctctt ataaatgatg gagccaatca gactgatata
    28021 gcaatgagta ttttcaatgc agttgtcgta caaactgtga gtgtcctttc ttgtggaaga
    28081 aaaatagaag gaaatgttgc gtttttgggt ggtccattgt actttttatc tgaacttaga
    28141 gaagcattta aaagagtatt agatttaaaa gatgaaaaca taatcttccc agaaaatgca
    28201 caactttata ttgctattgg agcaggatta ttatcagtag atgaagataa tatacaacta
    28261 aaatctctaa tagaaaaatt ggatagtata aagaatattg aagatggaga agtaaattta
    28321 ttagaacctt tatttaaaga taaaaatgag tatgaagaat tttcaagaag acatgaaaaa
    28381 gaaaaaataa attatgtgga cataaattca ataaaaagca attgttattt aggaattgat
    28441 gcaggctcaa ctacaactaa agcagcactt attgatgagg atggaagact tgtatattct
    28501 tattataata gcaatgaagg aaatccatta aaaacaacca ttaaagttat aaatgaagta
    28561 tatgatatat taccaaaaaa tattaaaata ttgagttcaa ctgtaactgg atatggagaa
    28621 ggtcttatta aaaaagctct taaaatcgat aatggagaga tagagactat agcacattac
    28681 aaagctgcta aatttttcaa caaagatgta gattttatac ttgatattgg tggtcaagat
    28741 atgaagtgcc taaaaataaa agatggagta attgataata taatattaaa tgaggcttgt
    28801 tcatcaggtt gtggctcatt tttagagaca tttgcaagtt ctttatctat gacaatagaa
    28861 gaatttgcgc atgaaggtat ttattctcaa aatcctgtag atttaggttc aagatgtact
    28921 gtatttatga attctagagt aaaacaagct caaaaagaag gtgcaagtgt tggagatata
    28981 tcagcagggc tatcttattc agtaataaaa aatgctttat ttaaagtaat aaagattaga
    29041 gatttaaatg agattggtaa taatattgtt gtacaaggtg gaacctttta taatgatttg
    29101 gtgcttagaa gctttgaaaa actaattgga aaaaatgtaa ttagacctaa tatttcagga
    29161 attatgggag cttttggttc agcacttata gctaaagaaa aatatgaaag tggatacaaa
    29221 acatccttgt tatctaagga agagttgaat aatatcaagc tagaagcaag tgtaactaga
    29281 tgtaaaggtt gttcaaatca ttgtcttctt acaataaata aattttctga taatgaaatt
    29341 tttatttcag gtaatagatg tgagaaaggt gaagctattt atggtgataa aaaaatagga
    29401 ttagaaaaga aaaaaccaat aaatttattt aagtacaaat acaatagaat ttttagatac
    29461 aaaccattgg aaaaagaaga agcaaaaaat ggggaaatag ggataccaag agtattaaat
    29521 atgtatgaag attatccatt ttggtttact tttttcaatg ctatgggctt tagagttgta
    29581 ctttcagata ggtcatcaaa acagttatat gaaactggta taactagtat tgcatctgaa
    29641 acagtttgtt atccaggaaa attagttcat ggacatatag aaaatttaat tgcaaaaggt
    29701 ataaaaaata tattttatcc aagtgtaaca aatgaaaata aagaggattc aaatgcagat
    29761 aattattata attgtcctgt tgtaatatca tattctgaag taataaaaaa caatgtagag
    29821 aatataagaa ataagaacat aaattatata aatccattta taagtctaaa tgacaaggaa
    29881 aaattgaaga aaagactcta tgatgaatta tcaagacatt tttctgatat aaaaataact
    29941 aaagaagaag ttaatcatgc agttgataaa gctactgatg aacagaattt atttaaaatg
    30001 gagatacaag cagcaggtga aagagcactt aaagaaataa aagaaagaaa tatgaagggg
    30061 attgtgttat gtggaagacc atatcatata gacccagaaa taaatcatgg aatgccagag
    30121 cttataaatt cattagatat ggcagtgctt actgaagact ctatatgtca tttagccaat
    30181 gtacaaagac cacttagagt tgtagaccaa tgggtatatc attcaagatt gtataaagca
    30241 gctagttttg taagagataa aaactatttg gaacttgttc aacttaattc atttggctgt
    30301 ggattagatg cagtaactac agaccaggtt caagaaatac ttaatgaaaa atcgaagata
    30361 tatactatta taaagattga cgaaggcaac aatttaggag cagctaagat aaggatgagg
    30421 tccttaaaag cagctatgtt tgagagagaa agcaaaaata tagatgtaaa aaatataaag
    30481 gtaaacagga tacagtatgc taaaaataat cagataacga acaaacatac tatattagca
    30541 ccacaaatgt ctccaataca tttccaattc cttgaaaaag ctgttaactt aagtggttat
    30601 aatatagaaa ttctaaaaga tactgatagt agtgtaattg aagaagggtt aagatatgta
    30661 aataatgatg catgttatcc agctataata gttgtaggtc agctaataac agcattaaaa
    30721 tctggcaaat atgatttaaa taacacttct gttactataa ctcagactgg tggaggttgt
    30781 agagctacaa attatattgg attcttaaga aaggctatat acgatgctgg atttaaagat
    30841 attcctgtca ttgcacttag tgtaaatgga atagaagata gtggtataat ggataatata
    30901 tcactaaaat taatcaatag actctttatg tctgttgttt atggtgattt gctgatgaaa
    30961 gtactatata gagtaagacc atatgaaaaa gttactggaa gtgcaaatgc tctatatgaa
    31021 aaatgggttg atatatgcaa atcttctctt gttaaagcaa aaatttctgt atttactaga
    31081 aatataaaag atatagtaaa agaatttgat aatttagaga tactagatat caaaaaacca
    31141 aaagtaggtt tggttggaga aatattggtg aaattccatc caattgcaaa taataattta
    31201 gtagatatat tagagcgaga aggtgctgaa gcagtcgtgc cagaccttac aaatttcttc
    31261 ttaagttgtg cctttaatac tatatacaaa cacactcatt tagaaggaag tagaaagagt
    31321 agaatgatag gagaagcatt tatatacata actggaatat atcaaagagt atataaaaaa
    31381 gcacttgata agagtgagag attttatgct ccagccaata taaaaaatgt tgctaaaagt
    31441 acagaaccag tagtgtcatt aggtaatcaa actggagaag gttggctttt aacaggtgag
    31501 atggttgaat tattaaatga aggtgtagaa aacattattt gtatgcaacc ttttggatgt
    31561 ttacctaatc acataattgg taagggttct ataaaagaat taaagaggtt gtataaaaat
    31621 gcaaatataa tacctattga ttatgaccct tctgcaagtg aggtaaatca acttaacagg
    31681 ataaaactta tgctttctaa ggcttttaaa aatatataag atttatattt tgatatgtta
    31741 aaagttaatg aatttaaaaa tatactctat attaagtatc tataaaaaag agttatccta
    31801 ttttttaggg taactcttta ttagttaaaa aattatatgt atactgatat taaagggggg
    31861 aagtaatatg agtttagtat atgatgttta taaatcaaaa ttaggaaatt tgtacatatt
    31921 aagtgaagat ggttttatag ttagtatata cataggagat gaaaaattta ataatttaaa
    31981 aaatgaatgt attaaaaaag gaattgatat aaagagaagt aaggaagatt taaaggaagc
    32041 aataagccag atagatgatt actttaatgg aaaaagaaaa gtctttaatt taaatattcg
    32101 tattgaaggg actgaatttc aaaaatcagt ttatagggaa atgttaaaaa taccttatgg
    32161 agaaacattg tgttattcag atatagcaag aaatataaac aatccaaaat cagtaagagc
    32221 aataggacaa gcaagtaaaa gtaataaaat tccaataata ataccttgtc atagagtagt
    32281 tggcaaaaaa gatattggtg gatatatggg gaatcattct gatttgaaag agatactatt
    32341 aaatttagaa agacagagtt aacttagaaa gaaatgtttt tattgttaat atttgatata
    32401 ataatagtaa aaagaagtat tttttacaaa aacaatgaag aatttatttt aatttataaa
    32461 aatgaggggg aattttaatg tgtgataaag ttatagcatc aaaatatcat gagaagggat
    32521 ttaattgtgc agaatcagtt ataaaagcat ataatgagga atttaataca gatataccag
    32581 tttgtttagg aagtggactg ggttcagggt gtggggttgc tagtttatgt ggtgcagtaa
    32641 atgcatcaaa tataataatt ggatatgtaa aaggcagaaa ccacgaagat gaaagtacta
    32701 aagctaaagt atatgctaaa gatttaacta caacagtaag aaaagagtat ggttcagagt
    32761 tatgtataga cctaaaaaaa gatttagttg cttgtagaga aataatggat ttcgcatatg
    32821 atagtctaaa ggaaacttta aaaaaagaat tataagaaaa taagctatca agtataaatt
    32881 atttgatagc ttatttttat agtcgatact aaataaatca aatatatttt agaattatta
    32941 tttgtaaata aacagcaaat aattttagga tttttattta atatagtttt tatttatatt
    33001 tatttttgaa tatgttatac tttgtttaaa taaatatttg taatgaggga gatgagaaga
    33061 ttgaagaatc tcaatttact tattaataaa ttatattcaa aaaatcataa tgaagcatat
    33121 aaaacttttt tattcttaga aaatgaaagt ttaaaatcaa atattacata ttgttttttt
    33181 gactcattcc tagaaatgat tgataacgaa aactcatata ttcgaacaag gggattgtta
    33241 ttaatctctg caaatgctca gtgggatatt gataataaaa ttgaaataaa tatagatagt
    33301 atactaagtc atatcgtaga taaaaaacct tctgtatcaa gaatgtttat taaatccata
    33361 cctaatatta caaagtataa agaaaatctt attaatcgca taaaaatgga attaagtaat
    33421 gcagatataa gtatttataa taataatatg aaaccattag tagaaaagga tat
//
LOCUS       DAMJVI010000035        33346 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.037, whole genome shotgun sequence.
ACCESSION   DAMJVI010000035 DAMJVI010000000
VERSION     DAMJVI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.037"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            177..833
                     /locus_tag="PC545_001737"
     CDS             177..833
                     /locus_tag="PC545_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456508.1"
                     /translation="MKKGDFIWSGVLICIIAVLAIPSSREVFMEATGAHPYIGGFLKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGVVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC545_001738"
     CDS             complement(1341..2612)
                     /gene="sleC"
                     /locus_tag="PC545_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HDF2456509.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTAYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRYFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVKIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            2857..3573
                     /locus_tag="PC545_001739"
     CDS             2857..3573
                     /locus_tag="PC545_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HDF2456510.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVIILWKINLDKLE
                     KYMNHSKKVFFGNLRKKKKK"
     gene            3652..4962
                     /locus_tag="PC545_001740"
     CDS             3652..4962
                     /locus_tag="PC545_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDF2456511.1"
                     /translation="MTELPNEFLEDIEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLELFDLNQIPWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINASRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSYAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRGENLYLLPE
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKESSGVIKNHYPKGLRKVF"
     gene            5062..5598
                     /gene="lepB"
                     /locus_tag="PC545_001741"
     CDS             5062..5598
                     /gene="lepB"
                     /locus_tag="PC545_001741"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2456512.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDIDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHTI
                     LGKVLVRLYPFSKIGTID"
     gene            5764..6300
                     /gene="lepB"
                     /locus_tag="PC545_001742"
     CDS             5764..6300
                     /gene="lepB"
                     /locus_tag="PC545_001742"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDF2456513.1"
                     /translation="MNETIKEEIVEWIKIIITALFFAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            6310..7065
                     /locus_tag="PC545_001743"
     CDS             6310..7065
                     /locus_tag="PC545_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDF2456514.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSELCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELKGFDLRYFNLHLGYV
                     MTNRVRKNRDKYLGNSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDAYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLNNIVGEG"
     gene            7139..8806
                     /locus_tag="PC545_001744"
     CDS             7139..8806
                     /locus_tag="PC545_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HDF2456515.1"
                     /translation="MKKINLKIEGKDKEYSLEENSPGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEADVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDGNKRVVLIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLEE
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            9347..10276
                     /gene="glsA"
                     /locus_tag="PC545_001745"
     CDS             9347..10276
                     /gene="glsA"
                     /locus_tag="PC545_001745"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDF2456516.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            10374..10937
                     /locus_tag="PC545_001746"
     CDS             10374..10937
                     /locus_tag="PC545_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456517.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            11425..12261
                     /locus_tag="PC545_001747"
     CDS             11425..12261
                     /locus_tag="PC545_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDF2456518.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     DEKDIIKFQEIRKENNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIERLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKKELKMIKEILS
                     SK"
     gene            12545..13540
                     /locus_tag="PC545_001748"
     CDS             12545..13540
                     /locus_tag="PC545_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2456519.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADEVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(13797..14201)
                     /locus_tag="PC545_001749"
     CDS             complement(13797..14201)
                     /locus_tag="PC545_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2456520.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYDISYNIFEK"
     gene            15042..15956
                     /gene="queG"
                     /locus_tag="PC545_001750"
     CDS             15042..15956
                     /gene="queG"
                     /locus_tag="PC545_001750"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDF2456521.1"
                     /translation="MDNKELKAFCNLIGLNCVGVAGVDKYDNLEKILKNRQQKGYLTG
                     MEEPIIESRINPRHIMKDANSIIVCAFPYHINRQEKNKNSNLSKYCHGKDYHIVVKDF
                     LQQICDYISKTVTDFKYKLFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSEK
                     EKVVLKNGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGRNIIKRNMEIILDKYDD"
     gene            16062..18299
                     /locus_tag="PC545_001751"
     CDS             16062..18299
                     /locus_tag="PC545_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2456522.1"
                     /translation="MKYNNTSEINPLDYILGQERAVEAMELGLKINNPAYNIYIAGEP
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQTYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEDNEEGIARFIAYQCDKNNLRHFTKSAVEEVIKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTKQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVSIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVRDGKFKIYTVERVEEAIEILTDVKFEEIKKLVKEKLISFSKIQ
                     TVSKE"
     gene            18497..19714
                     /gene="nth"
                     /locus_tag="PC545_001752"
     CDS             18497..19714
                     /gene="nth"
                     /locus_tag="PC545_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDF2456523.1"
                     /translation="MKNVNLKEINEKDVILEEEINKKGMDKKNIIEKEEINKKGIDKK
                     DIIEKEEINKKGIDKKDIIEKKEINKKGIDKKDIIEKKEINKKEMDKKDIIEKEEINK
                     KGMNKKDIIEKEEINEKEIDKMIGKKDEIIDEKDIENFMDIEEDKKSIKVSKKSTKKG
                     ETEKKTKKSSSNKATQKTSKETKSKFKIKNESANEKDVNKILDELEKLYPDAKCELNY
                     GTAFELLIATILSAQCTDVRVNKVTSELFKKYNTARDFANLSIEEISKEIKSCGLYKS
                     KSQKIKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVF
                     RVSNRIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIK
                     EDCNYYKELNETK"
     gene            19908..20378
                     /gene="trmL"
                     /locus_tag="PC545_001753"
     CDS             19908..20378
                     /gene="trmL"
                     /locus_tag="PC545_001753"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDF2456524.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPEPLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            20414..21262
                     /locus_tag="PC545_001754"
     CDS             20414..21262
                     /locus_tag="PC545_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2456525.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEREYKDRIDI
                     SGDEFYKKLREENVYPKTSQATYAQFKEIFEKYTNEDREILYIAGSATATGTYQSAVM
                     AKNDVDGKIYTYDSNLLCMGTGLLVIKAGELASEGKSVAEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMQDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            21601..22605
                     /locus_tag="PC545_001755"
     CDS             21601..22605
                     /locus_tag="PC545_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2456526.1"
                     /translation="MRFKRILILMLTVAMIAGMLVGCANNPSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFESGFREGVKFSNSSIKDISVEYADVFKDSK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIDAVRAKNKKVIATNKDQHELAPENVISSI
                     VKDFENPTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVNKNNK
                     "
     gene            22731..23843
                     /locus_tag="PC545_001756"
     CDS             22731..23843
                     /locus_tag="PC545_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2456527.1"
                     /translation="MKIKRILMACFVVVFTIFILAICNFDFGFDKVNKNNSNIDSAKS
                     KQSESKSLPTSAEKDKSNSKSKNKEDDTEDDSYVRKTIGVLLDTENLNNESYNNNVLS
                     ALKRADEDFDVRIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAATEYP
                     KQQFAIVDYTYKVQPANVISVSFQDNVTGYLAGLIAGKMTDTGKVGFIGGVEGSSRDK
                     YESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDSKRA
                     IQVARENGGKVIAINKDQYDMAPDNVISSIIKDVEQPVYELIKNVAKDGFKGSKVMEF
                     KIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            24333..25094
                     /locus_tag="PC545_001757"
     CDS             24333..25094
                     /locus_tag="PC545_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456528.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTIEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            25097..25915
                     /locus_tag="PC545_001758"
     CDS             25097..25915
                     /locus_tag="PC545_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HDF2456529.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            26095..26697
                     /locus_tag="PC545_001759"
     CDS             26095..26697
                     /locus_tag="PC545_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HDF2456530.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRAEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPSGIKNMIQDYLGEIIESEVKQSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIAQGLITMFI"
     misc_binding    27156..27393
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            27457..29376
                     /gene="thrS"
                     /locus_tag="PC545_001760"
     CDS             27457..29376
                     /gene="thrS"
                     /locus_tag="PC545_001760"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDF2456531.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSDEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            30144..31235
                     /locus_tag="PC545_001761"
     CDS             30144..31235
                     /locus_tag="PC545_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2456532.1"
                     /translation="MIEKVEEKSKIIKTSKEYKRTIIEEKDKEWTKKLNDILSRSSFS
                     NDELDKIKALIPKEILTNENVGEVVTEDGYAKPEYDEVFKSLFTLNNEYDLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRVETDPTINYIGEKRPKLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLISNPSNLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNDFDLSEDEIKEVYENN"
     gene            31905..33245
                     /locus_tag="PC545_001762"
     CDS             31905..33245
                     /locus_tag="PC545_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2456533.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKRISNKKINFCV
                     VGIIVFADIIYFFDVPPNIRVIISMIITIAFYKISYDVGFIKSVLISLIYWMLLIATD
                     ALSISLTVWLNSINDMSSMGTGDIYRIESIILGKILLIVSLLLYRALKIKLNIEINKR
                     TLFYIVIPIVANIASFFVIFEYIFNPGSGSLIHNFQFVMISVVLLISSITLVLIAKKL
                     QQYNTLVVENNMIKNTLKYENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDKNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDVMQNFYLIRVENTKMNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKAVNHVI"
BASE COUNT        13306 a         3141 c         6092 g        10807 t
ORIGIN      
        1 gataatggtg gatagagtag taagaaaata aatagataaa gggatagaat aaatttaaat
       61 atggaaaatg aatatctaat aataagtgat taataaataa ttgtagatag attgtaaatt
      121 aaataaatct ttaatagaaa ggagaactat ttatatatta aatagtattt ataaatatga
      181 aaaaaggcga ttttatatgg agtggagttc ttatttgtat tattgctgtc ttagcaatac
      241 caagtagtag agaagtattt atggaagcaa caggtgcaca tccatatatt ggaggttttt
      301 taaagtttgc tgtattagct acaatgggag atttacttgg tgctagaatt ttgtataagg
      361 attggaaaat accagtagga gtattttata gagcaatagt ttggggaata ataggtgtag
      421 ttaccacata tggaatgtca atctactcat caggtgtagg tcaagcacaa gtggatggta
      481 tgcttccatt tgagggaata gcttttgcaa atgctctatt taagagtgca actatgaata
      541 tactaatgtc accaactgta tttttattcc ataaggttat ggactgtttt atagataaaa
      601 aatatgaggt tggaaagggg attaaggtaa aacttaaaga tgtggtaaat attgtggact
      661 ggaatgctta ccttggtttt agtgttttaa aaactatacc atttttctgg ataccatgtc
      721 atacaattgt attcttattc cctgctgagt atagagtaat tgcttctgca tttgcatcta
      781 tagctttagg tctaatattg gctttagcta ataagtctaa agttgcagtt taggttcact
      841 gatgaagata gtagtattat ttaaattagg aataactttt taaattaata ttatttgtat
      901 ttataataaa taataattta tataagaagt aatattctaa gtgatggata gtgttttaaa
      961 taataagaag taatactcta agtgatagat agtgttttaa ataataagga gtaatactct
     1021 aagtgagaag tagcatttta aataataagt gagattttaa ataaaaagta ggtttttcgt
     1081 tagaatagca ttttattaaa cttaaaaata aatataatat tttaaagaaa tctaagttgc
     1141 taagcactgt ttttaagaag cttaaaatcc cccatttaaa gttttaagat tctaaaaaaa
     1201 caagttataa ctaaaaaatg gctgttaaga ctacaatact tataagtaat acttatggta
     1261 tatattagtt cataacagcc atttttattt taaattaata attttattca ataatttgag
     1321 tttatgttct aaatcaagct ttaaattaaa ggatttaaag aagctattct agttgtagct
     1381 acataaactc tggatatttc ataccatgtt ttaaaatcta ctactccgct ttgaggtaaa
     1441 ccaaagattc cttggaatat cttaacagcg ttttctgtat ctgttccata gataccatct
     1501 tctataactt ttggtacagc tggataagaa ttagatatag catttaactg attttgtatg
     1561 gttctaacat actgtccaga agaaccaact tgtagtgtag taccagggaa tgacactggt
     1621 acaccactta ctataggcgc tctttcaaat actatctcat ctccatagaa atatcttagt
     1681 atactctcat agtcatatcc ttggtcacct aagtctttac tgccccattg agtcatttgg
     1741 tcaggacatt gggatttttg tccatcacaa tattgtgcta atagtggctg tcttgatgta
     1801 ggaggtcttt ttataaaagt attaaaaata gcatctacaa ctacgttaat tggttcaaat
     1861 aagtttctat tatttataaa cttatggtcg tatgctgttg ttgaagttat tgtaaagtta
     1921 tatcctttat ttctatacca ttcagtaaag actctattta aagtaaatga tattatagct
     1981 attacatttg cataaattgt ctgttcaggc caagttgaat atatttcaga tgatgctatg
     2041 tttttaatgt actctttaaa tggaatccaa tagtttggag ctgatgaatc ttctggcaaa
     2101 ccatcatgaa ctactataaa ttcaggcact acagggttat ctaatacaac gaaaccagta
     2161 ggaggtggta atggttttaa gttactttct ggaatcttag gaggataagt accatataga
     2221 gtatgttctc ctatgtcaaa aatcaactca ctttgtcttg agaaagaacg cttacttctt
     2281 gttctaggag acatgggaac tccttgtctt gcttcaattg tagctaacaa ctgtgttcct
     2341 tctatgacaa ctgtctcata tccatctgct atggcttcca ctatatactg gctataaggt
     2401 ctaacatcag atggttgtag agagtaatct acatctggtg ctggtaatac taaaccagtc
     2461 acttgaccag attcattact tcttaggttt tggtataaag ttgatgagct ttggctacca
     2521 ttagacattg aatatatgtt tactactgca ttttgaatag gtctattgtt agtagcatca
     2581 attatgctta ctgttaagaa accatcttgc atcaaatcac cctttcttta aatgaattta
     2641 gttcccttat atttcaagat atgaatatag cttaaaattt gttaaataat atagatataa
     2701 atttaaattt ttttgagtat ttgagtatat ttgtatttaa ataatttaaa ataatttaat
     2761 gtatatttta gtatgtattt aaaacacgaa taagttaaaa ataagtagat gatataaatc
     2821 ataaataaat caaaaatttt gaaaggaaaa taatctatga agctagattt atttttatta
     2881 gcaatagttc ctatattgat aggaatgttt tggataaggt ctaaagatag atattgtaga
     2941 gaacccctaa tccatctaat taaatttttt ttaataggtg cttttctaag tgttataatt
     3001 atacttttag aaaatttact gatgaagttt aatgtatttg aagggtattc agaactaata
     3061 tatgtatcat ttgtagttgc aggacttgta gaagaaggtg taaaagctct gatactgatt
     3121 cctgccctaa ttaaagaaaa acactttact gaaaagttag atggtattat ttactctgta
     3181 tttttagctt taggatttgc aacaattgaa aatatggttt atattttttc tgaaagtaga
     3241 aatctagcac tgcaagttgg tattaatagg gcagttatct ctataccagc tcatgtaatg
     3301 tttgcaatta ctatgggcta ctatatttct aagtataaat ttgagggaaa taaaaataaa
     3361 agacgtgagt atttgtttat ggcagtgtta attccaattt tattacatgg agtttttgat
     3421 ttcatattga tgatagaata tagatgggct attatattac ttatagttta tgtaataatt
     3481 ttgtggaaga taaatcttga taaacttgag aaatatatga atcactctaa aaaagtgttt
     3541 ttcggtaatc ttaggaaaaa gaagaagaaa taaaatttaa aggaaagaca taaataatta
     3601 aagtataata aatgacacac aagtaaagca gattgaaggg ggattataga tttgacagaa
     3661 ttaccaaatg aatttttaga agatattgaa gaaatactaa aagatgaata tgatgatttt
     3721 ataaaaagtt atgaggaaag taagactact gggcttagaa taaatacttt aaaaattgac
     3781 aagaaaaaat tatttgattt ggagttgttt gatttgaatc aaataccatg ggcagaagaa
     3841 ggtttttatt atgatgataa aattgacaga cctggtagaa atccactgca tgaggctgga
     3901 gcatactatt tgcaagagcc ttctgcaatg agtgtaatac caaaggcaga tattaaagag
     3961 ggtgaaaaag ttctcgatat gtgtgctgct cctggtggaa aatctacata tatactatct
     4021 aagctaaacg atacaggttt attggtttct aatgaaatta atgctagtag aattaaggct
     4081 ctaggggaaa atttagagcg atttggagcg agaaattgta ttataacaaa caccgattct
     4141 aatagcttaa gaaagacgtt taatgggtat tttgataagg ttattataga tgcaccttgt
     4201 tctgggcaag gtatgtttag gaaggatgag gttgccatat cagattggag ttatgcaaag
     4261 gtagtagaat gtcaatccat ccaaaaagag attataagag atggatataa aatgttaaag
     4321 aaggatggca ttttaattta ctcaacttgt actttttcaa gggaggaaaa tgagcatgtt
     4381 ataaatgagt ttatcagtga atatccacat gcagaactta ttgaaatgga aagattatgg
     4441 cctcataaga taaagggaga aggtcatttt gttgccaaaa taaaaaaatt agaagaagaa
     4501 gaatgtaatg tcaaagaatt aaaaataaaa aatttagata aagaaataaa ggattatagg
     4561 gagtttgaaa agaaattttt aaaggttgat ttttatagta ataaaaaaaa taaattttat
     4621 ttaagaggag aaaatttgta cctacttcct gaaatatatc cagatacgaa aaaactaaaa
     4681 gtattgagat atggtttgca tttaggggtt ttaaagaaga atagatttga gccttctcat
     4741 gcactatctc attacttaaa agttgaagat gtaaaaaatg ttgaaaactt cagtgtacag
     4801 agtgaatcaa ttcttaaata tttaaaaggg gatgttgtaa attctaatga tagtagagga
     4861 tgggttttgg tttcggttga aggaatacct ttaggttggg gaaaagaatc ttctggtgta
     4921 attaagaatc attatcctaa aggtcttaga aaagtatttt aaactattag aaaaaagaag
     4981 tataatattt atattgtagg tttatggtag attattttgc tgtagattga cttaatattt
     5041 aacacagagg agacgatttt agtgggtgaa gcagttaaaa aagaagttgt agaatggata
     5101 aaagtgattg tcatagctct tgttttggca tttgcaataa ctcgttttat agtgccaaca
     5161 atagtcaaag gagaatcaat gtatcctaca ttagttgaac gtgattattt gatagttaac
     5221 agaattgcgt acaaggtagg agagccaaaa tacaaagata taatagtatt caaaaccgac
     5281 ttaacagagg aaaatggaaa gaaaaaagat ttagtaaaaa gagttatagg tgttcctggt
     5341 gaccatgtaa aaatacaaga ctccaaggta tatgtaaatg ataagttgtt agatgagact
     5401 tcctatatac ataataatcg tactgatgga gatattgata tcgtagttcc agaaggaaaa
     5461 ttatttgcaa tgggagataa tagagaaaaa agtttagata gtagatacga tgaggttgga
     5521 ttggtcgacg agcataccat tttaggaaag gttctagtca gattgtatcc attttctaag
     5581 ataggaacta ttgactaaac atataaaatt tggagcaatc gctgattagc tgaaatcacc
     5641 agaccataca caatagttgt ctatataaaa aagacaacac cttatttagt agacttatct
     5701 aaaagatagt gtttaagaga cattatcaat aaagataata taactataaa ggagaatttg
     5761 tgaatgaatg aaactattaa agaagagatt gtagagtgga taaaaataat tattactgca
     5821 cttttttttg catttattat aactcgtttt ataaaaccaa cattagtaaa tggagaatca
     5881 atgtacccaa cacttaaatc acatgattat ttggtagcaa acaggatgac atataagtta
     5941 tcagaaccaa aatgtggaga tataatgata tttaagactg atttattaca agagaatgga
     6001 aggaaaaaag agcttgtaaa aagggttata ggtgttcctg gtgaccatct aaaaattaag
     6061 gattctaagg tttatataaa tggtaagtta ttaaatgaag tttcatatat acatgataat
     6121 tatactgaag gcgatattga tatggtgatt cctaagggaa aagtatttgc gatgggagac
     6181 aatagagaag ttagtttaga cagtagatat aaagaagtgg gattagtaga tgaagaaaat
     6241 attaaaggaa aagttatttt aagagtattt ccttttacag atataggtat ttttgagtag
     6301 gtgatttgta tggcagttgg tatagcgtcc ttattgttta atattgagga ggctttaaac
     6361 atttgtgaga gtataaagca aataactcat ttagagattg gaattgacaa tatatctgaa
     6421 tgtagtgagt tatgtaaata taaagagaga atttctaagt taggattatc tatagggata
     6481 cacttgccta tggaattaaa tacttgcgaa aacatagagt atatacgaaa ttcatggata
     6541 aattttattg agaagataga atttgaacta aaaggatttg atttaaggta ttttaacctt
     6601 catttgggat atgttatgac aaatagagtt agaaagaata gagacaaata tttaggaaat
     6661 agtgtggatt ttttagataa attaaataca aattcatatg tatgtataga gaatacttat
     6721 tcaaaaggtg gggatttttc aaatattgga aatatatctt atgattttga atatatattt
     6781 aagaggatta aaaattctaa gatttgtttt tgttacgata ctggacactg tttaattgat
     6841 gaggatgctt atgtaaagaa cttaaaggat aaaataagac tgatacactt aagtgataat
     6901 gatggtatca atgatacaca tgttggtata ggaagaggaa ttttatctga agaggggatt
     6961 aaagaggtct taacattaga tgcagaatat ttagtattag aaataaatta cgaagacatt
     7021 gaagatacta taagtaaatt gaataatatt gtaggagagg gttaaatacc tctctttatc
     7081 ttgttgatat gcctcattaa ttaaaattta tattcatcaa aaggaaggag atgttgaagt
     7141 gaaaaaaata aacttaaaaa tagaaggaaa agataaggaa tattcattag aagaaaattc
     7201 ccctggaatc agattgggag atatagcaaa agaattttgt gatgagcata agggttatat
     7261 aacacttgca gttgtagaca ataaactaaa agagctaaat tgtagagtta agaaggattg
     7321 tgagataaat tttttagata ctactaatga agatggagag agagtttact ttagagtcat
     7381 gtcatttata tttgtaatgg catgtagaga aatcttttgg gatagcagag ttacaataga
     7441 acattcacta tcagatgggc tatattgtga agttcatata gatagaaagc ttaaagaagc
     7501 agatgttgaa aatataaaaa acaagatgaa agagatagta aacaacgatt atgttataga
     7561 aaaaattgaa gttacaagaa atgaagctat ttctatcttt gaaaataatg aaatgtatga
     7621 aaaggcagag cttttgaaat acaaagagta tgatagtgct aaagtatata agtgtagaaa
     7681 ctatatagac catttttatg gttatatgtt gccatcaact ggttatataa aatcatttga
     7741 tattaagctt tataatggag gcgttataat tttaggacct agtgaagaag ataagactct
     7801 tcctatgaaa tttgtacctc aacctaagct atcaagtgta tattatgaag cagaagaatg
     7861 gtcaagactt atgggaattg acaaggtagt atctcttaat aaaataatag aaaacaatga
     7921 atatggagat ataataagga catttgaagc attgcatgaa aagaagctat ctcaaatagc
     7981 ggatatgata aaggatggca ataagagagt agttttaata gcagctccat cttcatctgg
     8041 aaaaacaagt tttgctcaca gattatcaat acatcttaga gtaaataatt taaatcctat
     8101 ttcaatttct ttagatgatt attttataga tagaaaacat actcctttag atgaatatgg
     8161 aaattatgat tttgaatcaa tttatgcaat agatttagag aagtttaact tagacttaaa
     8221 gaaactttta gctggtgaag aaattgatga tataagattc aattttaaag aaggtaagag
     8281 agaatacact ggtaaaaaga taaagataga ttcaaatcag ccaattatat tagaaggtat
     8341 acatgggcta aatcctattt taacatcatc tataccagat gaagataaat ttaagatata
     8401 cataagtgcc cttactcaaa taaatttgga tgaccataat cgaatcccta ctgctgattt
     8461 gagaatgata agaagaattg ttagagacta taattttaga ggatatagtg ctgataatac
     8521 tattttacaa tgggcatcag ttagaagagg agaaaagaag aatatattcc cgtaccaaga
     8581 ggaggcagat gctatattta actctgcttg tgtatatgaa ttggcagttt taaaacgatt
     8641 tgcgaaacct ttgttggaag agataaagga agataatcct gcacatattg aagctactag
     8701 attactaaag tttttgcagt attttgttga attggacgat acttctgaca taccaggtat
     8761 ttcaattctt agggaattta ttggaggaag taaaatagtt gattaatgtg tgtattatag
     8821 attgttttat gaagattaaa tggcatagca agtagtgtta ttggtttatg ttaagtatat
     8881 taagtgtaaa gtggagaatt aaattgatac agattttaaa gtttgagttg atatataaaa
     8941 aggcttgttt tatacttttg tatgaagcaa gctttttatt ttaaatttta cttttttgtt
     9001 taatattttg ttagtttatg tgattatttt ttaaaagagt aaaagagtaa aagagtaaaa
     9061 gagtaaaaga gtaaaagagt aaaagagtaa aaggtgttgg gttagaaatt tttggtgtta
     9121 taaaaattta aattactgga ttattagatt gctggattgc taaattacta ggttgttaaa
     9181 ttattgaatt actaaattat taagctcttt aaactattta aattgtttga aaaatgaatt
     9241 gattcttgta gaaaataatt ttaaagtata atttttttta acattgtttt attatagtat
     9301 aaacttgtgc taaaatagaa tatataacat aatataagag gtgcatatga atacgttaga
     9361 tgagacatta ttaaaagaaa ttataagttc taataagaag tatactaatt atggacaagt
     9421 cgctagttac atcccagaat taaaaaacgc tagaagaaat gatttaggaa tttgtatcat
     9481 agatagcgaa aataatcttt atagtgctgg aaactgtagc acaaagttta ctatacaaag
     9541 tatttcaaaa ccaattgtgc ttgctatggc acttatggat aatgattggg aagacgtttt
     9601 ttcaaacgtt ggaatggagc caagtgggga cccatttaat tcaataatga aacttgaaat
     9661 taatgacaca aaaaaaccct gtaatccaat gataaatgca ggggctatag taaccacttc
     9721 ccttataaat gggagttgtt tagaagaaaa agaagaaaga atgcttagtt tctttagaaa
     9781 gttagcaaaa aatgataata taggaataaa ctatgatgtc tataagtctg aaaaaatgac
     9841 tggagataga aacagagcta tggcttactt attgaaaagt gatggattta tcagagggaa
     9901 tgtagaagat gttctagacc tttattttaa acaatgctca attgagatag attctgtaga
     9961 tttagcgaga attggaataa atcttgcaaa ttatggagta gatatagaaa atggtgagca
    10021 tttaatgagt gaaatggttt ctaggattgt caagacattt atgatgactt gtggaatgta
    10081 tgatgcttct ggggaatttg ctattaaagt tggcattcct gcaaaatctg gagttggggg
    10141 tggtataatg gcttctgtgc cgggacgcat ggggattggt gtttatggac ctgcgcttga
    10201 caaaaaaggt aacagcgtcg caggagttaa agttttagag gagttgtcaa ataaactcaa
    10261 attaaatatt ttttaaaata ttaagaaagg atttttttga caacaacata taatttaata
    10321 tataattaaa ttgtaaagaa atatgaatta aataaatttt ttaggagggg tcaatgaaag
    10381 aggtattggt attacgagat ttggagtgca taaaggctat tgcgcatcca cgtagaatag
    10441 atattctaaa agcatttgac aaatcgccat tgtcagcaaa acaattatct caattattgg
    10501 aagaacctca tgcaaaaata aactaccata taaaaatgtt atacaaagtt ggtattttag
    10561 aattagttga ggaaaaaata aagtcgggga ttgtggaaaa gtactattat ccaagtgcaa
    10621 aaaatgtggt tatagggaat aggatactta atttttcttt agacaatggg gaagaaaaag
    10681 aagaattgta catatcaaaa tttgaaaata tgagtgaagt tttttataag gcagcagagg
    10741 aagatgtttt agaaaatgaa aatatagttg attatcatga tatttctctt actcatgatg
    10801 aattagttga acttagtgac actatgaagt caaagataga tgaaatttta aataaaagac
    10861 aacacaatgt tgaaggttct aagtatgata tagctatggt tatagttcct actcttgagg
    10921 aggaatgtcc tagttagaga attaattatg gattaattaa agatataata ttagagattg
    10981 aacatcaaaa tttacttaat aatcaaaaaa tagtttttgg tatgggtaaa acaatgattt
    11041 ttaatctcta tttttttgtt tttttatacc tattgtattt gtaaataata tgtatagtgt
    11101 aagatgtata cactatctaa gaagtgctta gataatatac aaagtatgta agattataat
    11161 atttgaaatt gattaaaatg ggtataatta aagtgtaaat attaaaatta aaaattaata
    11221 taaattaaat caatgctaga aatatgttaa aagaggactg agattagttg ggatagaaca
    11281 tcccttagag cttacgaatt tttagtgaaa aagtactatc aaataggaat ctccatcttc
    11341 atatgttggc taaatgtagt taaggtggag tagttcaaaa ttaaaattga gtacaagtag
    11401 tagtacaaat aaaaggagat taatatgatt aaattaggaa cacatctttc aatagcaaaa
    11461 ggatttgcta atgcggctga gacagctgta tatattggtg caaatacatt tcaatttttt
    11521 agtagaaatc caagaggagg aaatgctaaa gaatttgatg aaaaagatat aattaaattt
    11581 caagaaatta gaaaagaaaa taattttgca ccactgcttg cacatgcacc atatactatg
    11641 aatctgggtg gtacaaagga tgatgtatac gaatttgcaa caagggtgat agatgaggat
    11701 ataagaagga tggactcatt aggaattgaa tacatgtgtt ttcatcctgg aagtcatgta
    11761 ggtggtggtg tagattttgg catagataga atagtaaaag gattgaacaa tgccataaag
    11821 ggtgatgaaa atataactat acttcttgaa actatgtcgg gaaagggaac tgaaataggt
    11881 tttaaatttg agcaattaaa aagtataata gacaaaattg agcataaaga aaaaataggt
    11941 gtgtgtcttg atacatgtca tattttttca gctggttatg atatagtgaa tgatttagat
    12001 ggggttttgg aagaatttga taaggttata ggcatagaaa gattgaaaac tgtccatcta
    12061 aatgatagta tgatgccatt tggagataaa aaagacagac atgctcctat aggggaagga
    12121 aagataggat taaaagcatt aattgatttt atggaacatc ctagtctaaa acatttacca
    12181 tttttcttag aaactccttt tgatgatgaa ggtcacaaga aagagcttaa aatgataaaa
    12241 gaaatactat ctagtaaata aacctttatt aaaaatatgt gaaatacttc accctcaaca
    12301 taattagggt ggagatacaa tcatgaaatg tgataattta aattaatgga taggaataac
    12361 aaaaattgta tacaaattaa ctgggtataa agtatgatta tattatgaat agactgatat
    12421 aaaatataat tatactatga atagtatgta aatatattgc aaagattctc ataatttgtt
    12481 gtataattaa agatgacata tttatattat ataaaaaatt attaaaagtt aggtggggaa
    12541 agatatgaga aatttaggca atactaacat gaaaataaag agagttggct ttgggggcat
    12601 tccaatacaa aggattactc aagatgatac taacttagtt ataaatgagt tagaaaaaca
    12661 aggaataaac tttattgata gtgcaagagg gtatacaata agtgaggaag caatcggaat
    12721 tgccattgaa ggaaaaagag ataaattttt cttagctact aaaagtatgt ccagagatta
    12781 tgattcaatg aagagagatg ttgaaataag tttaaataat tttaaaactg attttataga
    12841 tttatatcaa ttccataatg ttaaagaaga ggagtatgat aatcttttta aagataaaat
    12901 ggcttatagt gcccttttag aggcaaagga gcaaggtaag ataaaacata ttggtataac
    12961 tagtcacaat ttaaatacta tagaaaaagc tatagaagat ggaaaatttg acactataca
    13021 attcccttac aatatagttg aaggacaagc agatgaagtg tttaaaaaag ctcatgaaaa
    13081 aggaattggg ataatagtaa tgaaaccttt agctggaggt gctttagaca atgcaacact
    13141 ggctattaaa tatatactat ctaaagatta catagatgta gttataccag gtatggagag
    13201 tgttgaacag gttagacaaa atgtcgctgt acttgaaaat cttgtgctag atgaaaaaga
    13261 taataaggaa attgaagaaa taagaaattc tttaggtaaa aaattctgta gaagatgcga
    13321 atattgtatg ccttgtgctg taggaataaa tataccattg agttttttat gtgaaggtta
    13381 ttatactaga tatggattaa aagagtgggc taaagaaaaa tatgaggtta tggatgttaa
    13441 acctactgag tgtattgact gtgggctttg cgagagtaga tgtccatatg aacttcctat
    13501 aagagaaatg cttaagactg ttgttgaaaa gctaggatag attttattta ttgaaatcgg
    13561 tgtattaaat ttactccgat ttctttttat tagatgtggt cttatataca aatattaaat
    13621 ttacatagtt atagctatta tataaattta atatttgttt aatacttatt aaatatttac
    13681 ttaatgcttt ttaaaaacat ttgtttgagt atagacataa cacaattatt attgttgtat
    13741 tagtatagtt tcaaatactg gagtatcaat ataatgtcga ataacacata aaatctttat
    13801 ttttcaaaaa tattatagga tatatcgtat ttagatactg gataaagttc aatttcaacc
    13861 ctagggttat ctttatctat aaaatcaaat aaaagtactg gcttaagttg tttatcattt
    13921 tttattattc cacttttttc aataccatca caaatacttt ttggataatt atgtaaatcc
    13981 cccattctct tatttgggaa gaataatttc aagactgtta ttagattttc ttcaacagtt
    14041 tccccttgat actgttggtt tataaaacct gcaatcatgt tttcgtatgc aagatactta
    14101 gaatattttc cctttgaagg catccatgct tgaccattta tgttatgaag cttaaaatta
    14161 gatttactaa taggtctacc tggaattgtt attttaatca aatttttcac catccttata
    14221 cagtatttat tataatctgt atatgattta aaagcaaatt gttatttaaa gtgtttttac
    14281 agagcttgtt tgtaaaattt tccactttag atgtagtaat ataagataag tattttgctt
    14341 acaaatttta aaatttgata tatgatgggc atattcatat tagactatta taagaccttc
    14401 ataatattaa taagtataag tttagtaaag agagtaaaaa gaaatccata taagtcaagt
    14461 aaagacttaa taaatggcaa tgtaaatggg atattaatta taagaaaaaa tcaatctttt
    14521 tgcttaacta tagaaagaat gtcaaaatat aactaatgat atttgaagtt ttagagttta
    14581 tattaaaagg taagaaggtt aagtgggtaa aaaaggttga atagattaaa aaggctaagt
    14641 atctaaaaga gttaagtagg taaaaaagtt aaataagtaa atgaaaaagg ctaagtatct
    14701 aaaagagtta gtaggtaaaa aagttaaata agtaaataaa aaagatgaag tatctaaaag
    14761 agttagtagg taaaaaacta aataggtaaa aagatgaagt atctaaaaga gttagtaggt
    14821 aaaaaattaa ataggtaaaa agacgaagta cctaaaagag ttagtaggta aaaaaattaa
    14881 atagataaaa agacgaagta tctaaaagag ttaagcaggt aagaaaattg aatagctaaa
    14941 aagattaagt atttaaaaga gttagtagct agaaaaggtt aaaaagacta aaaaaattaa
    15001 ataaatttaa aaagtcaatt agattaaaaa ggaataattt tatggataat aaggaattaa
    15061 aagcattttg taatttaata ggattaaact gtgtaggagt agcaggggta gataaatatg
    15121 ataatttaga aaagatactt aagaacagac aacaaaaagg ttatttaact ggtatggaag
    15181 aaccaataat tgagagcaga attaacccaa gacatataat gaaggatgct aactctataa
    15241 tagtctgtgc atttccatat catataaata gacaagaaaa gaataaaaat tcaaatttat
    15301 caaaatattg tcatggaaag gattatcaca ttgtagtaaa ggacttttta caacaaattt
    15361 gtgattatat ttctaaaaca gtaacagatt ttaaatacaa gttatttgct gataatggac
    15421 cattagttga caggtattta gcttatttat caggaatagg atattttgga ataaataata
    15481 atatcataac tgatgaatat ggttcttacg tatttatagg gtatatagta aataattata
    15541 aatttgaaga agataaacca ctagaaaaaa gttgttttaa atgtggtaag tgtgtaaaat
    15601 attgtccggg aaatgctata ttgggaaatt atgatatgaa ccccaaaaaa tgtttatcat
    15661 atataactca aaaaaaagga gatttgtcag agaaagaaaa agttgtttta aagaatggca
    15721 aaaaggtatt tggatgcgat atttgtcaag aagtatgtcc acataatgtt gaaataccaa
    15781 caactcatat tttggaattt aaagaaaata taatagatta tttggattat gatgagatac
    15841 aaaatatttc aaataaagaa tttaaaagga gatatggaga tagagctttt agctggagag
    15901 gtagaaatat aataaagaga aatatggaaa taattttaga taagtatgat gactaataat
    15961 aagagtctgt ttcatatact ttaatgtgta tttactttaa cacttgttgt gtaaaagtca
    16021 tattatatca ttaaagttaa atatggggag gtgggggaat attgaaatat aataatacat
    16081 ctgaaataaa tccactagat tatatattgg gtcaagaaag agctgtagaa gctatggaat
    16141 tagggttgaa aataaataat ccagcgtaca acatatatat agctggtgaa cctggaactg
    16201 gtaaaagtac ttatgcctta aaggttttaa atgagtatgc atccaaaaaa aatacgcata
    16261 aagattggtg ttatatatat aattttgaaa accctagaga gcctattata gttgaattag
    16321 aaaaggggtt tggaagagaa cttaagaaag atatggagaa attaattgaa agtcttttag
    16381 aagattttaa aaatgcgttt gaaagtgaaa attttgaaat agaaaaaaat aaacttctgg
    16441 atgaatatga aatagaaaaa gatatgcttt tgaagcaaat taaaaaatat ggagaagaaa
    16501 aaggatttaa attaaaacag agtaaaatgg gaatagtttt tatccctttg aaagaagagg
    16561 aagatgaaag tgatgagtct gatgaagagt tttacaaagc taaaagagaa ctagaaaata
    16621 tggcgataca ggtcgtatat aaaattagaa acttagaaga aatagctgaa aaagcagtgt
    16681 tagagcttga agaagagata gctaaacttg tagtagaacc tcatatcaag agattgtgtg
    16741 aaaaatatga aaattatgat aagattcaaa cttatctgga aaatataaag aaagatataa
    16801 tagaatatat gtatttattc tatttagatg aagaggagtt aaaagataag tatgataaag
    16861 agcattttat taaatataaa attaacctat ttgtagacaa tggaagctca aagaataaag
    16921 aatcagcacc tgttgtagta gagataaacc caagtccagc aaatctattt ggaaaggcag
    16981 agtatgatta tgcaaatgga aatataaaga ctgatttcac aaaactatta tcaggagcat
    17041 ttcatagggc taatggtggt tatttagtac tttatgcaga ccaattacta aaatatacaa
    17101 tgtcttggga aattcttaaa aagactttgc agactaaaaa agtaatattg gaaacacaaa
    17161 cagcaattaa accagaaaat atgccattgg atgttaaatt agtactgatt ggaagccact
    17221 atatatatga tattttatat agatatgatg aagattttga aaaatatttt aaagtatttg
    17281 tagactttga tagtgaaatg gataaaaatg aagataatga agaaggaata gcaagattta
    17341 tagcatacca atgtgataag aataatttaa ggcattttac taaatcagca gttgaagagg
    17401 ttattaaatt tagcactaga ttatctggtg atttagaaaa attatcaact aagtttaata
    17461 aaataatgga agttgtaata gaaggaagtg cttatgcaga gtttagaaat tcggagtata
    17521 caaagcagtg tgatgttaaa aaagcaataa gtgaaaagag aaaaagaatc aatagagtgg
    17581 aaacacatat ggatgaatca attgaaaatg gatttactct aatagaaaca gagggaagaa
    17641 aagtaggagt gataaatggt ttgtcagtct taagtacagg agaatactct tttggtagag
    17701 cttcaaggat tacagcgacc actagcccag gaagtaaagg catagtaaat atagaaagag
    17761 aagtaaatat gagtggctct atacataaca aaggagtact tatattaggt ggctatcttt
    17821 ctgagaattt tgcacaggat ttactattgt cactaaatgc atatgtttgt tttgaacaaa
    17881 attatggtgg aatagatgga gatagtgcat cttctgcaga attatatgta ctattatcat
    17941 ctcttagtgg agtatcaatt aaacaaaata ttgctgttac aggttctata aatcaaaagg
    18001 gtgatataca agtagttggt ggtataagtg aaaagataga aggattttat tctatatgta
    18061 aaactaaagg gttaaatggt gagcaaggcg tcataattcc tagaaaaaat caaagaaatc
    18121 tcgttttatc agatgaagtt aatgatgcag tgcgagatgg aaaatttaag atatatactg
    18181 ttgaaagagt agaagaggct atagagatat tgacagatgt taaatttgaa gaaataaaga
    18241 aattggtaaa agaaaaatta atatctttca gcaagataca aactgtgtca aaagaataag
    18301 tttattatag aatttaatgg gtatttatgt ataataaaaa gcaagaactt taataggttt
    18361 ttgctttttt tgtaaatata tataaattaa attcttattt tgtatgtttt atatattgat
    18421 gtttattttt ttgttgatat gattaaacta tttataaata gcgttttata aattggtatt
    18481 ttgaaggggg ataattatga aaaatgtaaa tctaaaagag ataaatgaaa aagatgtaat
    18541 tttggaagaa gagataaata aaaaaggaat ggataagaaa aatataattg agaaggaaga
    18601 gataaataaa aaaggaatag ataagaaaga tataattgag aaggaagaga taaataaaaa
    18661 aggaatagat aagaaagata taattgagaa gaaagagata aataaaaaag gaatagataa
    18721 gaaagatata attgagaaga aagagataaa taaaaaagaa atggataaga aagatataat
    18781 tgagaaggaa gagataaata aaaaaggaat gaataagaaa gatataattg agaaggaaga
    18841 gataaatgaa aaagaaatag ataaaatgat tggtaaaaaa gatgagataa ttgatgagaa
    18901 agatatagag aattttatgg atattgaaga agataaaaaa agtatcaaag taagtaaaaa
    18961 aagtactaaa aaaggtgaaa cggagaaaaa aacaaagaag agtagttcta ataaggctac
    19021 acaaaaaaca agtaaagaaa ctaaaagtaa gtttaaaatt aaaaatgagt cagcaaatga
    19081 aaaagacgta aataagattt tagacgagtt agaaaaactg tacccagatg ctaaatgtga
    19141 gttaaattat gggacagcct ttgaactttt aatagcaact attttatcag cacaatgtac
    19201 tgatgtaaga gtgaataaag ttacttcaga gttgtttaaa aagtacaaca cagctagaga
    19261 ctttgctaat ttgagcattg aagaaattag taaagagata aagagttgtg gtctttacaa
    19321 aagcaagtct caaaaaataa aggatacatc tgaacagcta tgtgaattat atgatggtga
    19381 agtacctgat agcttagaaa agcttataaa attacctggt gttggaagaa aaacggcagg
    19441 ggttgtactt agtaacgctt ttaatcatcc agcaatagct gtagatactc atgtatttag
    19501 agttagcaat agaataggaa tagtggacga gccaaatcct caaaaaacag aatttgcact
    19561 tatggaagct ataccaaaag aaagatggtc acactcacat catgtattaa tttttcatgg
    19621 aagaagaatg tgtaaagcta gaaatccaga atgtgcaagt tgcccaataa aagaagattg
    19681 taattattat aaagaactta atgaaacgaa ataactttta gaaagtaagt tgtacaagag
    19741 ggattttctg atataataat acttgtgaaa atgttagata aaaaactatt atatacatga
    19801 aataaaaatt attattaaca ttattttata taatagtata atattattgt tattctgttt
    19861 ataaaataaa atttgataaa taaaagcgat acttagggag gattaaaatg tcattaaata
    19921 tagtattagt agaaccagaa atacctcaaa atacaggaaa tataataaga acctgtgcag
    19981 caacaggttc aactttacat ttagtaaaac cactgggttt ttcattggat gataaacacc
    20041 taaaaagatg tgggcttgac tattgggata tagctgatat acaatattac gatagctttg
    20101 aagaattaca agaaaagtat ccaaatagta aatacttttt ttctacaaca aaggctaaac
    20161 agtcacattc agatgtgaaa tatgaagaaa attgttttat agtatttggg aaagaaacaa
    20221 agggattacc agaacctttg ttgaaagcaa atatggatac ttgtattaga gtgcctatgt
    20281 tggatgttga aaaagctagg tcattaaatt tatcaaattc agttgctatt gtagtgtacg
    20341 aggctttaag acaaatagga tacccaaata tgaggtagtt tcaacaagcc taaatgagaa
    20401 aggagcatac ttaatgaaaa atctaaagat aatctgtgac agtttatcgg acgtgccaaa
    20461 agatttgtta gataaatatg atatagatat agtttcatta acagttatac tggaggaaag
    20521 agaatacaag gatagaattg atatatctgg agatgaattt tataaaaagc ttagagaaga
    20581 aaatgtatat ccaaaaactt ctcaagctac ttatgcccaa tttaaggaaa tttttgagaa
    20641 atatacaaat gaggatagag agatacttta tatagcaggg tctgccacag ctacaggtac
    20701 gtatcaaagt gctgtaatgg caaagaatga tgtagatggt aaaatatata catatgactc
    20761 taacttgctt tgtatgggaa ctggtttact tgtaataaaa gcaggagaac ttgcaagtga
    20821 aggaaagtct gttgctgaaa tattgcctgt tttggatgaa ttaaaagaga aggcttttgt
    20881 tatattttca gtagatacat tggaatattt acaaaaggga ggtagaatat catctactaa
    20941 ggcagcaata ggaagtatat taaatataaa accgattctt gaaataagag atggattagt
    21001 ttcgcaaata tctcaagtaa gaggaaagaa gaatgtaatt tctaagatga tggaattgat
    21061 aaaatcaaat tgtggtgatg atttatcaga ccaaattgta tatataggat atagtgatga
    21121 ttttaaggaa aaagaacaat tatctaaggt tatgcaagat gaattaagac ctaaagaaat
    21181 tagatttttc caaattggtg tgtgtatagg tgcacatgct ggtcctggtg ttactggtat
    21241 catatgctta aaaaataaat aatatatttt tggatgacat tatttatttt tttattaaat
    21301 taggatagaa tattaattat tgagatgtta aattggattt attttggatg aaattaagtg
    21361 ttgatatatt agatttagtt tgtagggaag tgtgtaaata gataagaatc tattgcacac
    21421 ttttttatag taagatttat taaaaaaatt tcaaaatgta cttgtaaatt gtatataaaa
    21481 aaattataaa taggatgtaa aagtaaatag gatgtaaaag taaataggat gtaaaagtaa
    21541 ataggatgta aaagtaaata ggatataaaa atatcgtaaa tgtatgagga gtgaaattta
    21601 atgcgtttca aaagaatatt gatattgatg ttaactgtag caatgatagc tggaatgttg
    21661 gttgggtgtg ctaataaccc aagcaataat cctagctcta gcaaagataa taaaagtgat
    21721 ataagcgata aaagtaattc aaaaaaatct gtatcaatga tactagatat tgaaggaaca
    21781 aataatgaag ctatgaataa ttcagcttta ttagcattaa acaatgctca aaagaaactt
    21841 aatatagata caaacaaagt ggaatcagat gattcctcaa ctttttctaa tagtatagat
    21901 attttatgta atgacaatta cgatttaatt atagctgtag gagctagatt tgcaaaacca
    21961 ttggagatgg tagccaagaa atatccaaag caacaatttg caattataga ctatgagtat
    22021 gacaagcaac cttctaatat aacttcaatt tcttatgaag ataataaatc tggatatcta
    22081 gctggattaa ttgctggaaa aatgactgaa agcgataagg ttggttttat aggaggaaca
    22141 aagagttcta gtagagataa atttgaatca ggttttagag agggtgttaa attttctaat
    22201 agtagtataa aggatatttc agttgagtat gcagatgttt ttaaagatag caaatcagta
    22261 gaatctatag ctaaaaaaat gatggataat ggagtggata ttattttttc tactactgaa
    22321 gatgattcaa aagctgtaat agatgcagta agggcaaaaa ataaaaaagt tatagcaacg
    22381 aataaagacc aacatgagtt agctccagaa aatgttataa gttctattgt aaaagatttt
    22441 gagaatccta cttataatct gatacaaagt tttgtaaaag gaaattataa aggcggaaaa
    22501 gttatagaaa acacagttaa gtctggaagt actgggcttg cccaaaatag ttctcaaaat
    22561 attcctccag atgtgttaga atatgttaat aagaacaata aataatctac tataatatcc
    22621 aagaatactt atgactttag ttatgaggtg aattggatgt tataatagac atatatagaa
    22681 agacatatgc agaggtaggt cagacctatc taaatcaggg agtgaatttt gtgaaaataa
    22741 aaaggatatt gatggcttgc tttgtagtag tttttacaat ttttatatta gctatatgta
    22801 attttgattt tgggtttgat aaagtaaata agaataattc taatatagat agtgctaaat
    22861 cgaaacagag tgagtctaaa agtttgccaa cttcagcaga aaaggataaa tcaaatagta
    22921 aaagtaagaa taaagaagat gacacagaag atgattcata tgtaagaaaa actattggag
    22981 tattgttaga taccgaaaac ttaaataacg aatcatataa taacaatgta ctatcagcct
    23041 taaaaagggc tgacgaagat tttgatgtta ggataaaact tgaaaaacca aagacttcta
    23101 aaagttttaa agagagtata gaaaaactgc ataagtatga accagatttg ataatagcag
    23161 ttggagccag atttgcagag ccaattaaag aagctgctac tgaatatccc aaacagcaat
    23221 ttgcaatagt tgactatact tataaagttc agccagctaa tgtaatttct gtatcatttc
    23281 aagataatgt tactggatat ttagcaggac ttattgcagg taaaatgact gatactggga
    23341 aggttggttt tataggagga gttgagggtt ctagtagaga taagtatgaa tctggattta
    23401 gaaaggggct taaaacttca aataaagatg ctgagttact agttaagtat tcaaatatat
    23461 ttgaaaaccc tgaatcagct agaactattg caaaggaaat gagagattct gatagagatg
    23521 ttgtattttc agctacagag gatgatagta aaagagctat tcaggtagca agagaaaatg
    23581 gaggtaaagt tatagctatt aataaagacc aatacgatat ggctccagat aatgtaataa
    23641 gttcaattat aaaagatgta gaacaacctg tatatgaact gataaagaac gttgcaaaag
    23701 atggttttaa gggaagtaaa gtaatggaat ttaaaataaa agatggagaa cttggcttga
    23761 cttcaaagag cagtagaaat ataccaccag atgttttgga atacataaaa aaagagtata
    23821 acaaagtgaa agatacatat taattattga tgtagaagat tataaaaata tactgcttaa
    23881 gtttagattg gtgataaaag gatatattta ttaaatttgt gctaactgta caaaatatat
    23941 tgattaagtt tagattagtg ataaaaagat atatttatta aatttgtgtt aacagtatgg
    24001 aatatactgc ttaagtttag attggtgata aaaggatata tttattaaat ttagtctaac
    24061 tgtacaaaat atattgattg agtttagatt agcaataaaa agatatattt attaaatttg
    24121 tgctaactgt acggaatata ctgcttaagt ttagattagt gataaaaaga tatatttatt
    24181 aaatttgaac caactgtaca aagctatact aattaagctt agattaacta tataaaatta
    24241 taacaataag tgtgtgggca gaaaaattct gctacacact ttatttatat tcacataaaa
    24301 aatttatcaa aaaaaagaaa aaggagtaaa aaatggaatt tacaagagaa ccatttatac
    24361 tagatgacga tttgtgtcta gctttggtgg acaaaagaat tagttatcta atggaaaatg
    24421 aattaaaatt aagaaatata gagataataa aaacaataga atgtaaagaa ttatatgaaa
    24481 gcataaaata tcacccggat atatgtattt gtaatttagg aaaaggagat ataattgttg
    24541 cgccgaatgt atataatcaa tacaaagagt tattaagtag atataaattt aatataatta
    24601 agggagaagc taatttatta aatagatacc cttatgatat accttacaat attgcgatag
    24661 ttggtgacta tgctatacac aattttaagt atactgacaa agcaatatta gattatattg
    24721 aaaataataa acttaaaaag ataaatatag agcagggcta ttctaaatgc tcaatctgta
    24781 tagtagatag aacgtctata ataacatcag ataaaggtat ttggaaaagt atgaagaata
    24841 cagaaataga gtgtttattg atagaaaaag gtcacataaa tttatttgag atgaattatg
    24901 gatttatagg tggatgtaca gggttgattt ctaaggataa actagctttt tgtggagatg
    24961 ttaaaaaaca tcctgactat gaacgtataa aaagttttgt agaaagtaaa aacaaggaga
    25021 tagttacact tagctgtgaa aatttactag atataggttc tatagttcct ctcatgacaa
    25081 gaaaggagcg ataactttgg ttgagaagga taatatttct tccatgaaat ttttgaggaa
    25141 taataatgta aacgatagat taatatatac gaatgacatt gtagaagtag tgatagattc
    25201 tgcaaagcaa gaagtgttaa ataaaaaaat acttaatggt actaaagctg aacaaagagc
    25261 tttaaatatt gatgagatat caacggaaat aactagtatg ataataagta taatgaaaga
    25321 gaaacttttg aaagaataca ttctaaaatg ctatgggcgt acatatcctg aagataaaaa
    25381 taatatatac atatattcac tgaacatatt tgctaaaaaa gagattttta tgagagaaac
    25441 agtatttact agggtgagaa attatataga agaaaatgat tttataaatg tagaaggttt
    25501 tattagattt agaatgagag aatttatgaa atatatttct gctataggtg atattgcagt
    25561 tgaggagtat ttaataaaga aagaccaaga tgaatttata agagttttaa aatactttat
    25621 agatactcaa gaagaaaaga ttgatttgct taaagttcat ataatggaag ataatacatt
    25681 tgttttatac gataaaaatg gaaataagat agacagtata gatgatgaag aaattataaa
    25741 tatggtaata agagaaaatt taaattatga ggacttttta ataagtacct tattaactct
    25801 atgtcccaaa aaaatagaaa tattggattt gttaaataat aattgctcaa aggaaattat
    25861 agatactgta gaagcaattt ttgaaaataa agttagtata attatggaaa actagaatct
    25921 aagcatataa aagctatcca tataaaatta aaaaaattct ttttaaatcg acacaaaaga
    25981 cttataattg tatataaagc tatagtataa ctagtgttat taagtataaa gaatatctac
    26041 ttgattaata caatatagct ggcaaatata caattgataa ggagtaattt tataatggat
    26101 aatttaatta ggatactgat attagctgtt attggtggat ttataggtta tgttacgaat
    26161 gtagttgcta taagactgat atttagacca atagagccta ttaaaatacc tattttaaat
    26221 atagaaatag taggtcttat accaaagaga agagcagaaa tagcagcaaa tgtaggagaa
    26281 ataattcaag aagaatttct ttctatggac gaaatacttg caaatatcat tactgacgaa
    26341 gataaggaag aagttgtaag atacataaaa gcaagggtaa aaattataat acatgagaaa
    26401 gtttcattta tcccgagtgg tataaaaaac atgatacaag attatctagg agagataatt
    26461 gagtctgaag ttaagcaaag tatagatgaa ttgagtaaaa atataataaa taaagcaaat
    26521 gaacgtatag atatacaaaa aatggtcgaa gataagatta atgaactaga tttatatgaa
    26581 ttggaagaga taataataag aatagctaag aaagaattaa aacatataga gtttttgggg
    26641 cttgtcttag ggtttttaat aggtatagct caagggttaa ttactatgtt tatttaaata
    26701 tatgagaagt agcatttatt taagtactaa ttatatttac ctatttgagt gaattactta
    26761 ttaatagcat ttagatatta attgtattta tttatttgat tgtgttactt attaatagta
    26821 tttaagtatt tactgtacta cttattaact atattaagta ttgattgtat ttaagtatac
    26881 aaaacctaaa aagagtacaa aaattagaag ttagttttaa taaaaattag ttttagtatt
    26941 tatctagtca tatctatcat gcatgtggat acgttttaaa aattttaaat ttatgaaaat
    27001 attataatat cactataaat caagagatta atttaatata aaaatataag aatattggtt
    27061 tacagctgat tgagatgaaa aataatgggt aattaatata ttacattaat gttgattgat
    27121 gtataactat ctgttataat gaaaatatat taaaaatcat agtgttgaag aggaagagta
    27181 gagtagaaac cattctcaga gaatttgggt ctatggctga gagcccatgt aataggtcta
    27241 tttgaaaact accttggagc tgagcttatt agcagtattt gttttgtact atacgtgaaa
    27301 gtgctcgggt gacgacctta tagtcatcaa gagggttgta tatacttgta tacgccaact
    27361 tgggtggaac cgcgaattaa tatcgtccca aacatatttg gggcgatttt ttattacaaa
    27421 aaatctaaaa attatttcaa taaggaggaa aaaacaatga ttaaagttgc tttaaaagat
    27481 ggctcaataa aagaatttga aaatgctatt tctgttatgg atgtagctaa atccattagt
    27541 gaaggattag ctagaaatgt ggtagcagcg tctgtgaacg gagaggttgt aggtctggac
    27601 cacattatag atactgattg tgacttgaat ttatttaagt ttgaagataa agaaggtaag
    27661 gaagttttta gacatacatc agcacatata ttagctcaag ctataaaaag actttaccca
    27721 gaagcaaagc ttgctatagg accaagtata gaaaatggat tctactatga tatagattta
    27781 gaccatagat tagttccaga ggacctagag aaaatagaag ctgaaatgaa aaaaatagct
    27841 aaagaagatt taaaaataga aagatttgaa cttcctagaa atgaagcttt agagcttatg
    27901 aaagaacaag gagaagacta taaggtagaa cttatatctg atttacctga aagtgaaata
    27961 atatctttct acaagcaagg tgattttact gatttatgta gaggaccaca tttaccatct
    28021 actaaaaagg taaaagctgt taaattacaa agtgtagcag gtgcatattg gcgtggtgac
    28081 gaaaataata agatgcttca aagaatatat ggaacttctt ttgagaaaaa caaagactta
    28141 gaagagtact tacatttact agaagaagct aaaaagagag accacagaaa attaggtaaa
    28201 gaattaggat tatttatgat acctgaagaa ggtccaggat tccctatgtt tttaccaaaa
    28261 ggtatggaac ttaaaaatga attattaaaa ttctggagag aaatacacag aaaagctgga
    28321 tatattgaga tagaatctcc aatcatatta aatagaaaat tatgggagac ttcaggacac
    28381 tggtatcatt acaaagaaaa tatgtataca gttaaaatag atgatgagga ttatgctata
    28441 aaacctatga actgtcctgg tggattaatt tattataact ctcaactaca ttcatataga
    28501 gatttcccaa tgagagttgc agagcttggt agagttcata gacatgagtt atctggtgca
    28561 ttacaaggat taatgagagt tagagcattt actcaagacg attcacatat attcatgtta
    28621 ccagagcaaa ttaaggacga aataaaagga gtagctaact taatagatgg tatatacaaa
    28681 acttttggat ttgaatataa tttagaatta tctacaagac cagagaactc tatgggaagc
    28741 gacgaagagt gggaagcagc agaaaatgga ttaagagaag ctttagaaga gttaggtcta
    28801 ccatatacta ttaatgaggg tgatggagca ttctatggtc ctaagataga cttccatcta
    28861 aaagattgtt taggaagaac ttggcaatgt ggtactatcc aattagatat gcagcttcca
    28921 agacaatttg ataatactta tattggtcaa gatggtgaaa agcatagacc agttatgatt
    28981 catagggttg cttttggtag tatagaaaga tttataggta tattaataga gcattatgct
    29041 ggtaaattcc cagtttggtt atctccaact caagttaaga tacttcctat atctgataaa
    29101 tttatggatt atgcaaatga agttaagaaa gagttatttg ataagggtat aagagttgaa
    29161 ttagatgata gagctgaaaa aattggattt aagataagag aagctcagct tgaaaaagtg
    29221 ccatacatgc taatagtagg tgaaaaagaa gtagctgata ataatgtttc tgttagatca
    29281 agagataaag gtgaaatagg tagtattaaa ttagatgaat ttatagctag tatttcaaaa
    29341 gaaattgaat caagagaaag tattattcaa gactaatata tattatatta gtagctaaga
    29401 tattgttata taagataagt ttaatcaata tgttgatatg ttgatgcata gtgattaaat
    29461 ttatgaaaag atgagtataa ttagaggtac agttaatcat atttttatga ttaatgtacc
    29521 tctagttttt tgtatggtag atttattaat aaatatatca tagaagtgtt ttgaacatgg
    29581 aaatatttat gtgcattctt taaataaacg tttatttttt gagtgataat taagtgcttg
    29641 ttggtaaggt tatggctgta aaaatcataa ctttattttc atatttgttc cattttcata
    29701 aaaattatta tccctttaag aaaatttctt aatcctcttg aaatatttcc tttttagtct
    29761 aaagttaaat ttctttcttc aaaatgaata tttaaatgaa ctaaaattta agtacatttt
    29821 ctaatcttat gagtctattt ttcatagata attgccctat aatagaagaa tctatgttgg
    29881 tgctatgtgg tgacaataac agcattgtca gtgattaatt aatgctttat aaatgattaa
    29941 ttagtgtttt atgttatttt tggacttacc ctctatacat agattaattt atttaacttt
    30001 agatagagag agaatgtcaa gtacatacac agacattaga gtttaagaaa gtgtttatgg
    30061 agcaagaact tttaaaagag gtagtgactt agtttaattt caagaaatga taaatacaaa
    30121 agaggaaaaa gagaggtgtt gaaatgatag aaaaagtaga agaaaaatct aagattatta
    30181 aaacatcaaa agaatataag cgaactataa ttgaagaaaa agataaagaa tggaccaaaa
    30241 aattaaatga tatacttagt agaagtagtt tttctaatga tgaattagat aaaatcaagg
    30301 cgttaattcc aaaagagatt ttgactaatg aaaatgttgg agaagttgtg actgaagatg
    30361 gatatgctaa gcccgagtat gatgaggtat ttaaatcatt gttcacttta aataatgaat
    30421 atgatttact tgtaaatttt ataaatgata ttctaaaaga tgctccatat gctaacagga
    30481 atatcaaacc gttcactcaa ataaagagaa taattagagt tgaaactgat ccaacaatta
    30541 attacattgg agagaaaaga cctaaacttg atattttggc tgaagatgaa gaaaataatc
    30601 atataaatat tgagatgcaa agagcttttg agaacgatta tttggaaaga gctgaatatt
    30661 atcttagtcg tgtacatggt agaaagttag aagaaggtaa agagtataaa gaaattggaa
    30721 aaactattgg aatacatata ctaaatcatg taaaatataa tcacatagaa gattatgtaa
    30781 attgcctgag attaacaatg gacgggcatc cagatattta ttctagtaaa actgcattat
    30841 attttataga gcttccaaaa attcataaat ctgactatat agtaaataga attatgctat
    30901 ggggaaaact tattagtaat ccttctaact tagatgtaag agttatagct aagaatgatt
    30961 ctattattaa aaaagcactt gatagattaa aagaattagg tagcaatgaa gaatatttat
    31021 taaatctgaa aagaggtgct tatatcatga ataaaagtag aaattttaaa gaagagattg
    31081 aaagagagac agcaattaga gttgcaaaag aaaaagatta taaaataatt ataatgttga
    31141 aaaaaaatgg ttttaaatta gatgacatat taaataaatt taatgatttt gacttaagtg
    31201 aagatgagat aaaagaagta tatgaaaata attgatgata aataaaaagt gaacttatat
    31261 aagtttgctg aatcggagtc tggaaatatt tatgtgtatt ttttaatgaa tgaaaattta
    31321 ttttaaaaac aataatttag aagtacagtt aattatattt ttatgatact gtacttctag
    31381 ttttttctat tgtagattta ttatttacta ataaatctac aatagaaatg cttttgttgt
    31441 aagaatcatt ttatctaatg attgtttttt ataatagtta aatttccatt tgtagtgttt
    31501 aaatataaat ctataacagc atttctataa atgttataaa tcactataat gattcaactc
    31561 aaagttactg cttaaagaag cagagatttt atcaaggtat atcatggaaa aaaatatttt
    31621 attgtaatat ttataagatt ttattatgga aataatgtaa aaactttata aataatatat
    31681 tttataccta gttatagata aatttatata gttattatta aaaaaagcaa taaaaagttt
    31741 aattcaattt agcaaattta gtgatataat taaaaaagaa aagaaatatg gacttatttg
    31801 gaataatttt gacaatctat ttactacacg tgtagagtcg tatataatta aattatacat
    31861 aaaatataaa aaaataattt agtaatacgt taggagaaag tattatgata aagtcaagct
    31921 tattttggaa tattttcatt gcgatatcct ttatgataga atggtttctt tgtaagaaaa
    31981 tgctagatta tacaagtgag aaaaggattt ctaacaaaaa aataaacttt tgtgtggtag
    32041 gtattattgt ttttgctgac ataatatatt tttttgatgt acctccaaat ataagagtaa
    32101 ttatatctat gataattact attgcatttt ataaaatctc atatgatgtg gggtttataa
    32161 agtctgtatt aatatcctta atttattgga tgttactaat cgccacagat gctttgagta
    32221 tatctttaac agtttggctt aattctataa atgacatgag tagtatgggg acgggagata
    32281 tatacagaat tgagtctata atattgggaa agatactttt gattgtgtct ttgcttttat
    32341 atagagcctt aaagataaaa cttaatatag aaataaataa aaggacatta ttttatatag
    32401 ttattcctat agtcgctaat atagcatctt tttttgtaat ttttgaatac atatttaatc
    32461 cagggtctgg tagcttaata cataatttcc aattcgttat gatttctgtt gtattgctta
    32521 tatcaagtat cactttagtt ttaattgcta aaaaattaca gcaatataat actttagttg
    32581 tagaaaataa tatgattaaa aatactttga agtatgaaaa tgagtattat gaagatatag
    32641 aaaagaaata tttaaaaact agattactaa gtcatgatat gaaaaatcac atcttgtgta
    32701 taaatgctat gattaaaaaa ggtattgatg ttacttctta tttaaataat ttgcaggtag
    32761 aaattcagag taatgacttg ttatttaata ctggtaattt tatattagat gctatattaa
    32821 gtgaaaagaa agaggtttgt gataagaatg atatagaact aaaaatagga ataaatttta
    32881 gttgttgtga agctgttgaa ttagtggata tatgtcatat attttcaaat attttagaca
    32941 atgcaattga ggcatgtaat aaaattgata gtggaaatag agttatttgt gttaaaggtg
    33001 atgttatgca aaatttttat ttaataagag ttgaaaatac taagatgaat aagattatta
    33061 ctaaaaatgg agatattttg acagacaaaa aaggttctct tcatggactt ggaataaaaa
    33121 gtgtaaaaaa ctctgtaaat aagtatggtg gtaatgttgt aattgaatat gatgataaaa
    33181 aatttgtagt taaaattgct attccactta gaaatgtgaa gaaagcagtt aatcatgtga
    33241 tttaaatgta tataaatttg atgtgtagaa atttaatata taagaattaa tgtataaaag
    33301 tgatgcatag aagtttaata tataagaatt aatgtataaa agtgat
//
LOCUS       DAMJVI010000036        30618 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.038, whole genome shotgun sequence.
ACCESSION   DAMJVI010000036 DAMJVI010000000
VERSION     DAMJVI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30618
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.038"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..884)
                     /locus_tag="PC545_001763"
     CDS             complement(<1..884)
                     /locus_tag="PC545_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2456534.1"
                     /translation="MKLKHIAIIGSLFPILFSLVLFFGVLISADSDDENSNFSSGITG
                     MNLSAEVLKHQTMVEKYARENGISEYVNVLLAIIQVESGGTAEDVMQSSESLGLPPSS
                     LDTESSIKQGCKYFASLLSSCKNQDIDDLNVAIQSYNYGGGYVGYVAGNGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVFGGSSPTTSFDCSGLTQWCYGKAGISLPRTAQAQYDATQHLPLSQA
                     KAGDLVFFHSTYNAGSYVT"
     gene            complement(881..3085)
                     /locus_tag="PC545_001764"
     CDS             complement(881..3085)
                     /locus_tag="PC545_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456535.1"
                     /translation="MKERIQSAFTKRKILHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFFVDNSWSWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGKSIQTLAGVTENGFSSTGFYVGFLLLIIL
                     VVGMYVAYTGLIKRETSKALHAVINFVVVFVLSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVQQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPEDEDGKTREEVVKTEIEDNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMEHRIARAVSAGGISGGVAGAVAGSAVAGKRAERKNTASKENRGNTTS
                     SMGQRAGSKVGAVLDTKNKVKDKANAVKENIKDMPTQTAYAVYSAKEKAKSSVSDFKR
                     GMVQEQQSRQTGRLEKQEQHKKSIAVKRMELQKAQEARQAQRKADGSATTGATRPHER
                     PATASTVSKTGAEKMQEVKRPATTPTTKASEPVKTSGVKERPLSSSTPDGKATQSTYR
                     QNVEKVTVSKETRQNYTKDRRTKVQQTQTVQKNQQTTEKIRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="PC545_001765"
     CDS             complement(3085..5535)
                     /locus_tag="PC545_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456536.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYEMIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKKLVTGKLREVAVQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNIKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEIHDFGYLMEHLYGRDGIAYEDYEYQLPKKKLKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNVIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYAVLFGGQALLLDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSENRGLLHVIEELRKEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSAYDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKDDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVQRNEVE"
     gene            complement(5513..5911)
                     /locus_tag="PC545_001766"
     CDS             complement(5513..5911)
                     /locus_tag="PC545_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456537.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLHKQILNETITAVRSVNYVPDKIY"
     gene            complement(6029..6532)
                     /locus_tag="PC545_001767"
     CDS             complement(6029..6532)
                     /locus_tag="PC545_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2456538.1"
                     /translation="MIDDMAVYIANLGRYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAVHDTDNFPIDIGEYVSIEELNEMYEMIEELPDYIVECLDEFISHYGTLEEVVEH
                     KDDIYFYPDCETMTDVAYYYIDELNALGDIPANLQNYIDYEAYGRDLDMGGRFIETSR
                     GICEIPY"
     gene            complement(6550..7053)
                     /locus_tag="PC545_001768"
     CDS             complement(6550..7053)
                     /locus_tag="PC545_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2456539.1"
                     /translation="MQETRVLVETGRTNGEETTSYWFDLPIDVAEFEEKLGVGAESQD
                     YRIIEKVLPYADEVHEHTSVYQLNELDFMYRQLSADMQEEYVELLTVFENLEALYICR
                     NVITVYHDCKSMIDVARQKLMNDPTFKHLSEDCQAYYFDFEAYASHLLENKRYLVTEN
                     SIFELPE"
     gene            complement(6972..7268)
                     /locus_tag="PC545_001769"
     CDS             complement(6972..7268)
                     /locus_tag="PC545_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456540.1"
                     /translation="MNDIFKDMQAKVGCEYISDLPSYKRKVWHEMKRLNPADYEEKQL
                     KDFSKYVFGMSYQTLKDVMKQQKGREEQCRKQGYWWKREEQMAKKQHHTGLTYR"
     gene            complement(7367..8140)
                     /locus_tag="PC545_001770"
     CDS             complement(7367..8140)
                     /locus_tag="PC545_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456541.1"
                     /translation="MKGIKVLMISDEMRVDILHAVFINKNFIKDEENCNYEKYLLELV
                     NKSIYFREKSNFAEYVPPKSENHGECDCNSPNYQMDFKLLESTTRLHASKELTGQIQK
                     FCDGVIGKCPPRRPNTQMTVTRLFASLRDYDCESLHSCLTEKYEYGTIEFDIQTYVKL
                     LTFKKNLFFFFPYKFSFNTCYNFKYALDSIRIALEKDFRESNLFREKYYAEYDTFLAY
                     IYEDNLIISKFEQDGKLQMIDCIYLFKSQTYSKLYEYTW"
     gene            complement(8200..8421)
                     /locus_tag="PC545_001771"
     CDS             complement(8200..8421)
                     /locus_tag="PC545_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456542.1"
                     /translation="MNFGQNLYQWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLV
                     VALIAVGLVFNAGGVKDVLLELFNKIIGA"
     gene            complement(8422..8556)
                     /locus_tag="PC545_001772"
     CDS             complement(8422..8556)
                     /locus_tag="PC545_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2456543.1"
                     /translation="MWELVKDFLLVSMGMGIGVVLMCILSISKEADYEMKQLEESEDN
                     "
     gene            complement(8569..9765)
                     /locus_tag="PC545_001773"
     CDS             complement(8569..9765)
                     /locus_tag="PC545_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2456544.1"
                     /translation="MVLNEEQWIKELREKRIAYGISQGRLAVASGITREYLNKIESGK
                     MKPSKELLETLHKELARFNPEAPLTMLFDYVKIRFPTLDIQHIIKDILKLNINYMLHE
                     DYGHYSYTEHYSLGDIFIYTSADEEKGVLLELKGRGCRQFESYLLAQQRSWYDFLMDA
                     LIDGGIMKRIDLAINDHTGILDIPELAEKCRKREYIGKSRSYKYYQSGELIKHREDDR
                     EYMGSTLYLGSLKSDVYFCIYEKDYEQYVKLGTPLEEADIINRFEIRLRNERAYYAVR
                     DLLTYYDAEQTAFSIINQYVRFIDEEPDKRKNDWKLNDRWAWFIGDNRQSLKLTTKPE
                     PYTLDRTLRWVQRQVAPTLKMLKKIDKGNGTDYMETIEKQAILSEKHEMIIKQQTTPA
                     KDLVES"
     gene            complement(9950..11344)
                     /locus_tag="PC545_001774"
     CDS             complement(9950..11344)
                     /locus_tag="PC545_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456545.1"
                     /translation="MRMILNKGHRIRASDKNLVYRFSMGTLLFVFVAVILLLNTKQLM
                     RTDWEHFSLLDNGFTLSLYNFITILIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSVFFTDLQSRSREKIVWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVRAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEQMKRHPNYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVGLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNLRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGFLAQARQ
                     DGTATCEAKGDGSD"
     gene            complement(11438..11875)
                     /locus_tag="PC545_001775"
     CDS             complement(11438..11875)
                     /locus_tag="PC545_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004056026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456546.1"
                     /translation="MCEECYSDENRITPLLNPLDCLENHTQYICGTCGRCICIEHDPN
                     RGLQRWNFPFKSLEIAKLYLRTADYTTKSSCGIYEIENSKGRVSYKIFAGNEDLHLFL
                     KKNKDKKCKQMTPVFNVGEYKEYPHTEVRKLTSDEIKQYMSER"
     gene            complement(11977..12360)
                     /locus_tag="PC545_001776"
     CDS             complement(11977..12360)
                     /locus_tag="PC545_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2456547.1"
                     /translation="MRLSNGFVIDKEKTFGELKFTAVRDVFLQNEDGTPSTQLKKRIY
                     DLKCSLHGGIIPVSVPPEVPLREFPYNAVVELVNPVADTVSRKTYTGADVDWYVKAED
                     IVLKNKGNQNTGTSQNHTPQGQPKK"
     gene            complement(12376..12702)
                     /locus_tag="PC545_001777"
     CDS             complement(12376..12702)
                     /locus_tag="PC545_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2456548.1"
                     /translation="MEMKYVVPDMAQSFGTLEFAGESEPIFERDKNNRKVIARRSYNL
                     YSDVQKGENVVVEIPVQAGEKHFKYEQKVKLVNPKLYGRGYAIGDVGHTDYVLVADDI
                     VAVEEK"
     gene            complement(12828..15872)
                     /locus_tag="PC545_001778"
     CDS             complement(12828..15872)
                     /locus_tag="PC545_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2456549.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPSTAVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYGEAHKELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSSNTSITDGNG
                     NYSIAGATYGVFSDKDCTKQLATLTTDKNGNTDVVEVKAGTVYIRELSAPAGYKVDTT
                     VYSLKVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDNPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTIAETDSDGTTHYITKLADAYKVSGDSFYMQDGKAILPL
                     GTLSVEETKAPNGYLLDGAYMQAGDKSEQIKGLYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETSDTKPQGSATLKDTAFDVISLNDNSVLVEGKLYKKNEVVKTIR
                     TDIEGVASTSADLLPYGKFRIVESEAPDGYLTDGAKPIDFAITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSAEWTSHK
                     HNTNAGKTSEDGVWFGTSEADDSKGALPYDTYIIEEMRCDSNKGFELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEISIHTTATSKDSEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAELIIDGKRVENDYTFIADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPVKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKEIEAGKDVTIIDTVT
                     LEGLEVGTQYKLVGWQMLKEENAELLINGKRVESDYTFTADSETMKAEVAFTFDATSL
                     DGKQLVTFEELYDLSNPDEPKKVTEHKDIEDEGQTITFNEKPEVPEKLETPQTPEKPN
                     RPSDSPKTGDSTNVMAFLVMLLTSAGGLAGTYLYKRRTMKKS"
     gene            complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="PC545_001779"
     CDS             complement(16115..17479)
                     /gene="rlmD"
                     /locus_tag="PC545_001779"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2456550.1"
                     /translation="MLSKNEMYIVDIVDIGQGGVGVGKYNGITVFVEGGLIHDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGIPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGRENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYANLNDTDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNIHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMNPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHIETCVLLQ
                     RRTM"
     gene            complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="PC545_001780"
     CDS             complement(17654..19414)
                     /gene="pyk"
                     /locus_tag="PC545_001780"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDF2456551.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIVCVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRIIKNGNACVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSAADITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="PC545_001781"
     CDS             complement(19466..20425)
                     /gene="pfkA"
                     /locus_tag="PC545_001781"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDF2456552.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFAGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(20634..24203)
                     /locus_tag="PC545_001782"
     CDS             complement(20634..24203)
                     /locus_tag="PC545_001782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDF2456553.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     LDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSNEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKVAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFIE
                     LEDLYGAIEIIVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFADRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="PC545_001783"
     CDS             complement(24360..25307)
                     /gene="whiA"
                     /locus_tag="PC545_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDF2456554.1"
                     /translation="MSFSTETKNELARVISGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILEDKEGFFTINKISENLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKS"
     gene            complement(25324..25749)
                     /locus_tag="PC545_001784"
     CDS             complement(25324..25749)
                     /locus_tag="PC545_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDF2456555.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(25818..26924)
                     /locus_tag="PC545_001785"
     CDS             complement(25818..26924)
                     /locus_tag="PC545_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDF2456556.1"
                     /translation="MAKLLIIVGILGWIALIVSLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDENLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="PC545_001786"
     CDS             complement(26936..27793)
                     /gene="rapZ"
                     /locus_tag="PC545_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDF2456557.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(27824..28537)
                     /locus_tag="PC545_001787"
     CDS             complement(27824..28537)
                     /locus_tag="PC545_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2456558.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYTDINILLGMECNILDDKGNIDMDDKFIRDFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVINAISNNDIFIITHPGDKGDV
                     YIEEVAKTAKKTNTRLEINSSHSFLNVNQLNKIKGLENKFIIGSDAHCPERVGDFELA
                     MKTISDSNLDISLVENIII"
     gene            complement(28541..29455)
                     /gene="murB"
                     /locus_tag="PC545_001788"
     CDS             complement(28541..29455)
                     /gene="murB"
                     /locus_tag="PC545_001788"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDF2456559.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKEESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRLMDM
                     EGNIFELSNEQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(29728..30498)
                     /locus_tag="PC545_001789"
     CDS             complement(29728..30498)
                     /locus_tag="PC545_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2456560.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMATASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSIVVGGIYYLCYVKNS"
BASE COUNT         8498 a         6651 c         4556 g        10913 t
ORIGIN      
        1 gttacatacg aaccagcgtt ataggtggaa tggaaaaata ccaaatcgcc agcctttgcc
       61 tgcgagagtg gaagatgttg ggtagcgtca tactgggctt gtgccgttct cggtaaggag
      121 ataccagctt ttccatagca ccattgcgtc agtcccgaac agtcaaagga agtggttgga
      181 gaagaaccac caaacacata tttccaccct tgatatttca acgcttcatt cattaccttt
      241 tgtgccagct ctcccgatac ctgcggaaca gttaaatact gattgactaa ttccacatag
      301 aacatatttc catagccata ccgccagcct ccgttcttcg caacggctat cgggttggta
      361 taggttactt tctttccacc cgatttctca cgggcgaagc tctctgcaag attaaaagtg
      421 tgtttctttc cgtttcctgc cacatagccc acatagccac cgccatagtt ataggactgt
      481 atcgctacat tcaaatcgtc gatatcttga tttttgcagg aagaaagcag ggacgcaaaa
      541 tacttacacc cctgcttgat tgagctttcc gtatctaagg aactaggcgg taaaccaaga
      601 ctttctgaac tctgcataac atcttctgcc gtaccgccac tttctacttg aatgatagcc
      661 agtagtacat tgacgtactc ggagataccg ttttctctgg cgtatttttc taccatagtc
      721 tgatgtttca aaacttctgc ggataagttc atacccgtaa tgccagaaga aaagttgctg
      781 ttttcgtcgt cgctgtccgc actaatcagc accccaaaga aaagcaccag agaaaagaga
      841 ataggaaaca gacttccaat gatagcgata tgtttcagtt tcattttttc ttctgtcctt
      901 tcttcgttac aagattacgg attttctctg tggtctgctg gttcttctgg acagtctggg
      961 tctgctgaac ctttgtccta cggtctttgg tgtaattctg gcgtgtttcc tttgacacgg
     1021 taactttttc tacattctgc ctatatgtcg attgggtcgc tttcccgtca ggagtactag
     1081 aagatagcgg acgttctttg acaccgcttg tcttaactgg ttcgcttgct ttcgtagtcg
     1141 gagttgtggc agggcgtttg acttcctgca ttttttccgc acccgtcttt gaaacggttg
     1201 aagctgtggc tggtcgctca tggggacggg tagctcccgt tgtcgctgat ccgtcagcct
     1261 ttcgctgtgc ctgccttgct tcttgtgcct tttgaagctc catacgcttg acagcgatac
     1321 ttttcttatg ctgttcctgc ttttccaagc gtcccgtctg tctggactgc tgttcctgca
     1381 ccataccacg cttgaaatca gacacgctgg actttgcctt ttcctttgcg gaatacaccg
     1441 cataagcggt ctgtgtgggc atatccttga tattctcttt gacagcgttt gccttgtctt
     1501 tcactttatt tttcgtatct aagacagcac ccacctttga gcctgcacgc tgtcccatac
     1561 tggaagtggt attgccccga ttttctttag aagccgtatt ttttctttcg gctctcttgc
     1621 cagcaacggc acttccagct accgcaccag ccacaccgcc cgaaataccg ccagcactta
     1681 cagcccttgc aatacggtgt tccatacgcc tagcccgatg tcgcatgaac agatacggtc
     1741 tgcggaatat cctgcgtccc atgctttgac tgtcgccagc gttgagagaa aacatactca
     1801 ttaagtcgcc cagcttcata tagataccag cgaaacatac tatctgcaag aacgccacca
     1861 tgaaaaacgg atagtccgtg gatatgttat aaaacatact ggaaatgcta aacgcaaccg
     1921 ttacaatgag cgttattcct gcccgtgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggaaagtag aaagctgata ggtaaaaaca
     2041 tagcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtaat accaagatta aagaacaaga ggaagaacac catacctaaa cggtttacca
     2161 cctgcggtat cgtcagattg ttgttgtcgt tgtcctcgat ttctgttttc acgacttctt
     2221 ctctggtctt tccgtcctcg tcctctggac ttgccgatac gagagcttcg acacggtctg
     2281 tcccgatttc ctctgcgttg ctgtttccaa actgtaagag tagccacggc tgttgtactt
     2341 gaatagaaaa taggctgtcc cgtatcaagt ctacgctgtc cttgccctcg ctgtcagagt
     2401 tggggagcat gatttttgtt cccaaatcaa gcgaagcggt actgatgtct gatgaaaatt
     2461 catttatctt cttgatgtag tcgggagcgt aggcaataaa cgaagcggac agcacgaaca
     2521 ccaccacaaa gttgataaca gcgtgaagtg ccttgctggt ttcccgtttt atcagtcccg
     2581 tataggcaac atacattccc accactaaga taataagaag caggaaacca acatagaagc
     2641 ccgtagaaga aaagccgttc tcggtaacgc ctgcaagggt ctgtatgctt tttccgatac
     2701 tgtctgccat atcgttaata aaatcaagtt tataggcttc ctgcaccaca tagccagttg
     2761 cattgcttaa ataaaggctt atcgtccaga caaagttggt aatgcagtaa agcccgtact
     2821 gtaccgattt tccgataccg tccagccagt tccacggaag ccacgaccag ctattatcga
     2881 cgaaaaaatc aagctggtag ttggaaagcg gatattttga atatagattt tctgcgttta
     2941 tggtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggaaagtgc caccacgaac agagccattt tgagaaagtg aagtatcttc ctttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtacgg gcggtctagt
     3121 atcaaaggcg tgtagtaatt cttcaaagac tgggtgtatc tgcaccacac cgacacgccc
     3181 gtataagtcc tgcaataaac attgtccgtt ctccaaatca cgcaagcgtt tctggttgtt
     3241 ttcgtcgtcc ttgtcgatac cgaaaaattc taaggtctgc tttatctcgt tgatgtcagt
     3301 agaacgaaat gcaaacttca aaccgatatt gtttttcaga ctttcctttg acacgtcata
     3361 ggcagactgg gtaacgaaat aaacgcctgc ctgcatagct cgtccagcac gaaccagctt
     3421 atttgatagg gtttcgcctt gtgccacatt taagaacgcc cacgcttcgt ccaaatctac
     3481 aatcttaaaa atgcttctgt ccgaatggat aaaatcaagg gcaaaggtac taatcacaat
     3541 cagcatagac accgacaata attcaatggt cgtgtattcc tcaaaagtgg tatctttatc
     3601 tggcagtaca aggtctgcta cttgaatgat attgagctgg ttatcaaggc ttatagcatt
     3661 ttctaccgtc ccgtctgaaa acagcaggtg tgcaaagtcg tagtccgtga agctgtcgat
     3721 atggtctgcg atattgcgtg agatagcggt atcttctttt ctcaattcct caataacgtg
     3781 taacagtcct ctattttcac tctgggtaac agaacgcacc gcttttcgta acacggggaa
     3841 tttttcgccg tccctagagg aaatacccgt aaggaatgtt aagatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaatgggtca agaagtcctg cattgtcctt
     3961 gtcgctggta aggtttacga tattgatttc atgggctatc tctgggagcg tttctttcca
     4021 gttgccacgc tcacttttag ggtctaacag aagtgcttga ccgccaaaca gcaccgcata
     4081 atatacaagt agattgttgc agaatgactt tccaccgcca agacttccca caaaagcaga
     4141 agcaagggca ttagtaactg tgccttttac tccctgcgaa gctaaagacg gttgcaggta
     4201 cacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgggtcgca ccaaaaccaa gcccagccaa gaagtctgac tttacatact gcacatagtc
     4321 attgatatat cgcttgctgg caggtaaaaa ttcagaatga agtcccaaca tatcgccagc
     4381 aggacgcacc agttttacat tgaggtcgtc gtaaaagtcc ttgacttcat cacaacggcg
     4441 tttcagctcg tcaagatcgg gtgcagacac ccgtatgacg tagcttaact tatacatact
     4501 ttctttgctc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtccaccac
     4561 atttgagctg gtttcacttc ctgcttgata ggcgtgatta tccaaatctt tcaattcctt
     4621 tttcttgttg cggactgtcg ttaatgcctt acggttctcc acgatttcta cattcataga
     4681 agtatcaacg gggaatgtga attgctgttg ctggaaatag aagatttcag aggacggaaa
     4741 atccaactcc ccgacaatta cattgacggt aaaataggac acatagcttt ccttgtcctc
     4801 atgttccaat cgtaaatacc gctggctttc ctcaatcaca caccttgtcg gacggataag
     4861 gtcgtagtat tttatcagcg tttctttctt caatttcttc tttggtagct ggtactcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc atgagatacc caaaatcatg
     4981 gatttccaag cgacgcacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aaatcattca tcagcgtgtg
     5101 gttcacttca tgaagaaatt ccttgaacgt cagccatgcc gattttttga tgttcttcag
     5161 attgagctgt tcttccgtaa ccatgagctt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatttttt ggactgccac
     5281 ctcacgaagt ttgcctgtta ccagtttctt tgactgctcc tgcatacttc taatggagct
     5341 ttctgtggca atctgcaacg catgaatttt tccctcacgg gactgtgcga ttagctgtcg
     5401 aaaactgtca tgcacgataa atttctgctc tgcggataga aaagaatagt tgtacggtat
     5461 catctcatag taggcgaaca cctcattgtc cttgttccag acaaggttat tgtcaatata
     5521 ttttatcggg aacatagttc acactcctta ctgccgtgat tgtttcattc aatatctgct
     5581 tatgcagttt tacggctttt cctgcataag tgattttagg tcgcaaggca taggttatct
     5641 gtgatttcaa aaagctgtac ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgagagcaac gggaataccg aagtatttga gaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ccgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggtaag ttaaagtcat tgattgcata taagactttt tccacgttcc
     5881 agatacccgt gtagctttta atctttttca agttctttca gcccctttcg taagttcttc
     5941 aaagagaaaa ggacagccat tttcagactg tcccttaaag agaaatacgc tgtcagtagc
     6001 aacaatgctt gttgctgatc ttccagcgtt aatatggtat ctcacagata cctcggcttg
     6061 tttcaatgaa tcgaccaccc atatccaaat ctcgcccgta ggcttcatag tcaatatagt
     6121 tctgtaagtt agctggaatg tccccaagtg cgttcaattc gtctatgtag tagtaggcaa
     6181 catctgtcat ggtttcacaa tcgggataaa aataaatatc gtccttatgt tccacgactt
     6241 cttccagcgt cccatagtgg ctgataaatt cgtccagaca ctctacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcttcaat ggaaacatat tcccctatgt
     6361 cgataggaaa gttgtcggta tcatggacag cgtattcttc gtactgttca ttcaagccga
     6421 ttttttcttt tacatcttcc tcgtcaatag ggaaagtgaa ccacgcaccg actaaatatc
     6481 cctcattgta tctgccaaga ttagcaatat aaaccgccat atcatcaatc ataagcacca
     6541 cctcatttcc tattctggca gttcaaagat actgttttcc gttacaagat agcgtttgtt
     6601 ttcaagtaag tgagaagcgt aggcttcaaa atcgaaatag tatgcttgac agtcctcgga
     6661 taaatgtttg aatgtcgggt cgttcatcag cttttgcctt gcaacatcta tcatgctttt
     6721 acagtcgtga taaacggtaa tcacgttcct gcaaatataa agtgcttcta agttctcaaa
     6781 cacggtaaga agttctacat attcttcctg catatcggct gatagctggc ggtacataaa
     6841 atctaattcg ttaagctggt acacgctggt gtgttcgtgg acttcatcag cataaggcaa
     6901 caccttttcg ataatgcggt aatcttgact ttctgcaccg acacctaact tttcttcaaa
     6961 ctctgcaaca tctatcggta agtcaaacca gtatgatgtt gtttcttcgc catttgttct
     7021 tcccgtttcc accagtaccc ttgtttcctg cattgttcct cacgtccttt ctgttgtttc
     7081 atcacatctt ttaaggtctg gtatgacata ccaaacacat atttagaaaa atctttcaac
     7141 tgtttttctt catagtcggc agggttcagc cgtttcattt catgccacac cttacgcttg
     7201 taagagggca ggtcggaaat gtattcacag ccgacttttg cctgcatatc tttaaaaata
     7261 tcgttcattt catcactcca atctgaaatt taggtaagaa aaaagcagtc aatcctaaga
     7321 ctaactgctt tgtgtgtaag tatgaatttg tttaagtgta actgatttac catgtgtact
     7381 cgtaaagttt actgtatgtt tgacttttaa ataaatatat acaatcaatc atttgtaact
     7441 tgccgtcttg ttcaaattta cttattatca gattatcttc atagatatat gctaagaaag
     7501 tatcatactc tgcatagtat ttttctctaa aaagatttga ctctctaaaa tctttttcta
     7561 aagcaattct tatactgtct aaagcatatt taaagttata acaagtatta aaactaaact
     7621 tgtatggaaa aaagaaaaat aagtttttct tgaaagtaag caatttcaca taggtttgta
     7681 tatcaaattc aatcgttcca tactcatatt tctctgtaag acaagaatgt aaactttcac
     7741 aatcataatc ccgcaacgaa gcaaataacc tagttacagt catttgtgta tttggtcttc
     7801 tcggtggaca cttaccaatt acaccatcac agaatttctg tatttgacca gtaagttctt
     7861 ttgaagcatg aagtcttgtt gtgctttcaa gtaatttaaa gtccatttga taattaggtg
     7921 agttacaatc acattctcca tgattttcag atttaggtgg tacatattct gcaaaatttg
     7981 atttttctct aaaatatatt gatttattaa ctaattccaa caagtatttt tcataattac
     8041 aattttcttc atctttaata aaatttttgt ttatgaatac tgcatgtaaa atatccactc
     8101 tcatttcatc tgaaatcata agcactttta ttcctttcaa aaaataattt atcgtaccca
     8161 cattataaca ttctcatatt acagcaacaa taaaattcgt tatgctccaa taatcttatt
     8221 gaacagctct aacagtacat ctttcacacc gccagcgttg aatactaagc cgacagcaat
     8281 aagggcaact accaagaagc caataagttt tgaaaactca cgcttgaacc ctaagtagat
     8341 accgataacc acaatcgcca taagcactaa gctctgtgca tttgataaaa accattgata
     8401 caaattttgt ccgaaattca tttaattgtc ctcgctttct tctaattgtt tcatttcata
     8461 gtcagcttct ttgctgatac tcaaaataca catcaagact acgccgatac ccattcccat
     8521 agataccagc aggaagtctt ttactaattc ccacattttt tatcactcct aactttccac
     8581 caaatctttt gcaggggtcg tctgctgttt gattatcatt tcatgctttt cactcaagat
     8641 tgcctgcttt tcaatcgttt ccatgtagtc tgtaccgttt cccttatcaa tctttttcag
     8701 cattttcaag gtcggtgcta cctgtctttg tacccaacgc aatgtgcggt ctaaggtgta
     8761 aggttctggt tttgtcgtca gtttcagact ttgccgatta tcgccaataa accaagccca
     8821 gcggtcattg agtttccagt catttttccg cttgtctggt tcttcatcaa taaaccgcac
     8881 atactggttg atgatagaaa aagcggtctg ctctgcgtca tagtaggtca gcaaatctcg
     8941 tactgcatag taagcacgtt catttctaag ccgtatctca aaacggttga taatgtcggc
     9001 ttcttccaga ggtgtaccta atttgacata ctgctcatag tccttttcat agatacagaa
     9061 atacacatct gatttcagcg aaccaagata aagggtactc cccatatatt ctctgtcgtc
     9121 ctctctgtgc ttgataagct cgcccgactg gtaatactta tagcttctgg actttccgat
     9181 atattcccgt ttcctgcatt tctccgcaag ctctggaata tccaaaatgc ccgtatggtc
     9241 gttgatagca aggtcgatac gcttcataat gcctccgtct atgagtgcgt ccataagaaa
     9301 gtcataccag cttctttgct gtgccagcag gtaactttca aactgcctgc aaccccgacc
     9361 ctttaactct aaaaggacac ctttttcttc gtcagccgac gtatagataa agatgtcccc
     9421 taatgaataa tgctccgtat aactgtaatg tccgtaatct tcatggagca tataattgat
     9481 attcagtttt aatatgtctt tgatgatgtg ctgtatatcc agcgtgggaa aacgaatttt
     9541 cacataatca aacagcatgg taagcggtgc ttctggattg aaccttgcca gttctttatg
     9601 caaagtttcc agaagttctt ttgacggctt catttttccg ctttctatct tgtttagata
     9661 ttcccttgtg ataccagaag ctaccgccag cctgccttgt gagataccgt aagcaatccg
     9721 cttctcccgt aattctttta tccattgttc ttcattcaga accatacccc caatctgtca
     9781 agtgtgaagt tttttaaggc gacttcacag ccgatttttg ctaggaaacc atgccagaag
     9841 ccttatgttt cctatgtttt tttgtctatt gtctatttgc aaacgtaccc ctctgttaga
     9901 taccgagggg ttttacctgc tggcgtggct tacgccacac cagcaacggc tagtccgagc
     9961 cgtcgccttt cgcttcgcac gtcgccgtcc cgtcctgcct tgcttgtgca agaaaaccga
    10021 ttgtctgcaa aaaatcatgt cctttcggga ctaaaggcgt gtaaaattca cttatgacgc
    10081 ttgttcccac atcacaatag ccacgccctt tgatacgctt ttggaaaaac tgctttttca
    10141 catctgaacc aaacagcata ccgtaaccta actcgctgat acgccccaat cccacacgta
    10201 aattgaagtt atctctgata ccgtccgaga aatattttgc gtctggacgc tggcaggcaa
    10261 cgataaggaa ataacctgct tgtcgtccta acatgacgat tttctttaac tggctaagta
    10321 agcccacact ttcttttgtc cccagcattt caaaaaacgc cacatattca tcaaagataa
    10381 gaaagcaggg tggcagtccc agataggcgt aattttcgcc cgtcttatag ttcgggtgtc
    10441 gtttcatttg ctcgcttcgc tgtaccatgc cctcataaaa agcattgaca cagtctatca
    10501 tttcttcttt ggtgtgatac acgtttccca tgactgttcc caagtctgct aagtccgcat
    10561 ttttcgggtc taagatgtaa aggacagcgt tggtatgcag tagggcttca atgagcgtca
    10621 gaagaaaata tgttttaccg ccaccagtcc caccagcaat cagagcgtga gggagtgcgt
    10681 catattccca gacaagattt ttcatcagtc taagacagcc gttctctgcc cgtacttcat
    10741 caatggtaat gcggttcgct atcatatcat aaagcagggt atattcgata tagccgtcat
    10801 gcagggtctt gtcggtcagc tcacaataca agccactttc caatttatcc tctaaccgta
    10861 aaagctggtc ttgatatttc cccagcgtaa tttcacagcg gatatgaagc agtccctttt
    10921 ccatttgata ataaatcttt ggaaaccaga cgattttttc ccttgatctg ctttgcaggt
    10981 cagtaaaaaa aacgctgtct tgtacggtat cagcttcata ccacttgttt tccagtatca
    11041 ttcgtgccag cttttgacgg tgcagtagct ttttgaaact gtcgtaacag aagcggtaat
    11101 acagaaaagc gaccaatgca caaacaccag tcgctatcag tatggttatg aagttgtaaa
    11161 gggaaagcgt aaagccattg tctaataagc tgaaatgttc ccagtcggta cgcatgagct
    11221 gttttgtatt taatagcagg ataaccgcta cgaacacaaa caggagtgtc cccattgaaa
    11281 agcgatagac aaggttctta tcgctggctc tgatacggtg tcctttgttt aaaatcattc
    11341 tcataaatca atcactcctt tctgggtatg aaaaaagcag tcaatcgtat agactaactg
    11401 ctttgtgtgc atggatatga agttgtcaaa ctggaattta acgttcactc atatattgtt
    11461 ttatttcatc agaagtcaat tttcgtactt ctgtatgtgg atattcttta tactctccaa
    11521 cattaaacac aggtgtcatt tgtttgcatt tcttgtcttt attctttttc aagaatagat
    11581 gtaaatcctc attcccggcg aatattttat aagatactct gcctttactg ttctctatct
    11641 cataaatacc acatgaactt ttcgttgtat aatcggctgt tcgtaaataa agttttgcaa
    11701 tttccaatga cttaaaagga aaattccatc tttgtaatcc tctgtttggg tcatgttcaa
    11761 tacaaatgca acgtccacaa gttccacaaa tatattgagt gtggttttct aagcagtcca
    11821 agggatttag taatggtgtt attctatttt catcagagta acattcttca cacatttttc
    11881 ttttcgctcc ttatcaaatt ctaatttgtc gctttcttca tacccatatt ataacagttc
    11941 cgtatatcct ctacaacgaa aatctattca gcaagtctat tttttcggtt gtccctgcgg
    12001 tgtatggttc tgtgaagtgc ctgtgttctg attgccttta ttcttcagta caatatcttc
    12061 tgcctttaca taccagtcca catctgcacc cgtataggtt tttcgtgata ccgtatcggc
    12121 tacgggattg acaagctcca cgactgcatt gtaaggaaat tcccttaacg gtacttctgg
    12181 cggtacggac acggggataa ttccaccatg cagactgcac ttcaaatcat agatacgctt
    12241 tttaagctgg gtactcggtg tcccgtcctc attttgcaag aacacatcac gcaccgctgt
    12301 aaattttaat tctccaaatg ttttctcttt gtcaatgaca aatccatttg ataatctcat
    12361 aaattcttca ctcctttact tttcttcaac tgctacaata tcatcagcta ctaatacata
    12421 atcggtatgt cccacatccc cgattgcgta ccctctgccg tataacttcg gattgacaag
    12481 ttttactttc tgttcatact tgaaatgctt ttcgccagcc tgcacgggaa tttccaccac
    12541 aacattttcg cctttctgta cgtcagaata aaggttatag cttcgtctgg ctatgacctt
    12601 gcggttgttt ttatcccttt caaagatagg ctcgctttcg cctgcaaatt caagagttcc
    12661 aaaagactgt gccatatctg gcacgacata tttcatttcc atattgtttt acctcgtttc
    12721 tttctatatt tgataagttt atgattgtaa ttttttattc tggcttgtct gccgtttgac
    12781 agtttcggcg gagaacgtga tttttctttc atcataccgt taccgcctta tgattttttc
    12841 attgtacggc gtttgtaaag atatgttcca gccagtccac cagcagatgt aagcaacatc
    12901 acaagaaatg ccatgacatt tgtactgtcg cccgttttag gactgtcgct aggtctgttt
    12961 ggcttttctg gtgtctgcgg tgtttcgagt ttctctggga cttctggttt ttcattaaaa
    13021 gtaatcgtct gtccctcgtc ctcaatatcc ttatgctcgg taactttctt cggctcgtct
    13081 ggattgctta aatcatacaa ttcttcaaaa gttacaagct gtttgccgtc aagggaagta
    13141 gcgtcaaagg taaaggcaac ttccgctttc atagtttcgc tgtcagcggt aaaggtgtaa
    13201 tcactttcca cacgtttccc attgataaga agttctgcat tttcttcttt caacatctgc
    13261 cagcctacaa gtttgtactg tgtaccgact tccaagccct ctaaggttac ggtatcaatg
    13321 attgtaacgt cttttccagc ttcaatctct ttgttgccgt ctttgtcggt agcagtcgta
    13381 tgtatcttga tgatacgttc tgtaatcaat acagtttgtc cgtcgtcctc aatgtctttg
    13441 tgttccgcaa ctttcacggg ttcgtctgga ttgcttaaat cgtataattc ctcgaatgtt
    13501 accagatttt taccgccaag ctcggacgca ttgaatgtat aagaaatttc cactttcatt
    13561 tcttcatcat cagcgataaa agtatagtca ttttccacac gttttccgtc aatgataagc
    13621 tctgcatttt cttcctttaa catctgccag cctttgagct gatattttgt accttttgta
    13681 agcccgtcta atttgacagt atcgacgatt gttacctctt ttcctgcaag gatagtcttt
    13741 tcgctgtctt tgctggtcgc tgtggtatgg atagagattt ctttttcgta ttcatcagtc
    13801 aaagtcccta aatcaatcac aaggttattt cttgatacca cgatttcaaa aggtgggata
    13861 agttcaaagc ctttgttact atcgcaacgc atttcttcaa tgatgtaagt atcataaggc
    13921 aatgcacctt tgctgtcgtc tgcttcagaa gttccaaacc acacaccgtc ctcgctggtc
    13981 ttgcctgcgt tagtattgtg cttatgagaa gtccattcag cagaagtgga gaattgcccg
    14041 ttatcatcag taactacaac atgactttct cccgtcgtct tgcttgtgat cctaaaggga
    14101 acatcagcaa gacgcttgtg tgtgcctgca ccgattttca ctccctcaat atccccacgc
    14161 ttaatctgat tgtagataga gtgggcttcg tcggttaagt ccacgatttt tccattctct
    14221 gtgattgcaa agtcaatcgg cttcgcaccg tctgtcaagt aaccgtcggg agcttcactc
    14281 tcaacgatac ggaatttccc ataaggtaaa aggtcagcag aagtagaagc cacaccctca
    14341 atatctgtac gaattgtctt taccacttca tttttcttat atagcttgcc ctcaaccaaa
    14401 acagaattgt catttaagga aatgacgtca aaagcagtat ctttcaaagt ggcacttcct
    14461 tgtggtttgg tatcgctcgt ttctaaatct cttttctgaa ttttcacacc gccacggata
    14521 accttgtctg atacggaaaa ctggttactt cccgtcaata cagcaaggtc gccgtcctct
    14581 gtaatttgtg taaggtataa gccttttatc tgttcagact tatcgccagc ctgcatatat
    14641 gcaccgtcca gcaagtagcc gtttggagct tttgtttcct caacagatag tgttcctaaa
    14701 ggaagaatcg ctttgccgtc ctgcatatag aagctgtcgc cagatacctt gtaagcgtcc
    14761 gctaattttg tgatgtagtg agttgtcccg tcactgtctg tttcagcgat tgtctttgta
    14821 acccatgtac gagtagcttc ggcagggaga ttgtctttgt tatagaagcc agcataatac
    14881 ttccatgtaa actctgcacc ctctaaagaa gcgtttccct gcggattgtc tttctgcgtt
    14941 tccatatcaa tcttgaaaag ctcaatcaag gtgtccgtta cttttggtgt atctgatacg
    15001 ttcaaggtcg ctgtctttcc agcttcaact tttaaggaat acacggttgt atcaacttta
    15061 tatcctgctg gtgcggataa ttccctaata tagactgtac ctgcttttac ctctacaaca
    15121 tctgtatttc cgtttttatc agtcgtaagg gtggcaagct gtttcgtgca gtccttatca
    15181 gaaaagacac cataggtcgc accagcgatt gagtaattcc cgttaccgtc tgtaatgctg
    15241 gtattactgg aagtcttttg cagttttgca tttccgacat ttaacttcgc ccagaattgt
    15301 cccaattcct gcccctcgcc agagtagata taaccgccac attcatagcg tcctttattt
    15361 tctttcacaa aggctttagc accagcgaaa acttcgtcct gagttgcttt tggtatttca
    15421 tcataagaag ctcgcacgtt gtcgcattgc cagccaagat gtacgctcaa tctctgccag
    15481 acaacacatt gttccaacag atagacttgc ttgtagttca attccttgtg tgcttcgcca
    15541 tactgtttga catactctaa ggataacgcc acatctgaaa tctggtcggc actcatacgg
    15601 gtgcttgcgt cagctctggt cttgtagcca ttcttaaaat ctgtattgat gtctatacag
    15661 taagcgtcct cgccctcaac agtcaaatgt ccctcgttaa aggtagaacc aatcgaaccg
    15721 tcattcatta ctttttctac gataccgaca cgttcctctg attccgtcca gtattgcttg
    15781 ctttctgcat ggacggcggt actcggtaaa gcagtaacga cagtcgcaaa ggcaagtact
    15841 cccgtacaca atcgtttcaa tatctttttc ataattctaa atgctccttt cattttgggt
    15901 aaaaaaatag acgctcaata ttgaacgtct tacattgaaa agggatactt tttacaagta
    15961 cccctcaaat ctaatattta attgtttgtg ttcccacact ctatatcaac aactactgaa
    16021 acattgatat agttttttat ctctttattt ctgattttat cgccctcgct cgtaaagact
    16081 atatgcttaa aaaagtgcct aaaattaagg atttctacat agttcgtctt tgtagcaaca
    16141 cacaagtctc tatatgtgta gaatgaggga acatatctac aggttgtgcc tcaacacatt
    16201 taaatccgtg aacatttaga taatctaaat cccttgctaa agtagaagga ttacaagaaa
    16261 catagacaat tctttccgga ttcattgaaa caatagtctc taataccttt tcgtcacatc
    16321 cctttctagg tgggtctaca acgactacat tggcagtctt accctctcta tacatcttag
    16381 gtacaatttc ctctgccttt ccaacataaa attcagcatt atgtatattg tttaatttag
    16441 catttatctt agcatctttt atggcatcat ctataatttc tattccataa acctttttag
    16501 ccttttgtgc caaaaataaa gatattgtac ctatcccaca gtaaatatca aaaacagtat
    16561 cagtatcatt taaatttgca tattcaagag ccttatcata caatacttta gtttggacag
    16621 ggtttacttg gaaaaatgat aatggtgaaa tctcaaacac taagtctcct atataatcgt
    16681 ttatttttcc atctccatat ataactatat tttctcttcc tagaatagca ttcgtatctt
    16741 tttgatttac atttacaacc aaagtcttaa atccaggtat attttcctta agcactgaag
    16801 ctaattcttt aagatatgga agtttgtttc catttgctac taatacaacc attacttctt
    16861 tagttgtaaa acctattttt gttactaaat gcctaatcaa ccccgtgtga gtcttttcat
    16921 catatatgct taccttatag gcccttatat atgtttttat tattttaaca attttatcat
    16981 ttatctcatg ttgtattacg catttttctg tcgatattac atcatgagtc ttctttttat
    17041 agaagccaat aattggaata ccattattct tttgtattgg aaattgtgct ttatttctat
    17101 atctatatgg attatccatt cctatagtgt catgaacttc tacatcctct aacttaccta
    17161 ttctcgaaag tacttgttta acttcatttg ttttcatttc aagttgttta ttataatcta
    17221 agtcttgtat ctgacaccct ccacaatccc taagattgtc actacacact cttttcaccc
    17281 tgaatggaga cttttctatt atctcaacaa tatctcccac agcataattt ttctttactt
    17341 tattaatcct aactttaact ttatcatgta ttaatccgcc ctctacaaaa actgtaatac
    17401 cattgtactt tcctactcct actccgcctt gaccaatgtc tactatatct acaatataca
    17461 tctcgttttt cgaaagcata aatcaccctc caatcaatct attatattta aatctttaca
    17521 aacttttttc tttaaattta tattaaaata ttgctattaa ttaaactata ttttttacta
    17581 agctacaata ttttatttta ccaaaaaaaa aggagcttat aaagcccctt agtaaataaa
    17641 tttattattt ttcttataaa actctagttg aacctctata taccacgcct cttgatgcat
    17701 ccacagttac aacttctcca tcttttacta gatttgttat atcagctgca gacacaataa
    17761 ctggtatatc taaatttata cctacaatag ctgtatgtga tgtcattcca ccattctcag
    17821 ttactacagc tcctgctttt tctatataat tgttcatttc agcatcagta gcagtagaaa
    17881 caagtatgtc gccttcatta aagtctacac aagcatttcc attctttatt atacgaactt
    17941 taccttctac tgtttgagta cctacaccta taccttgcac gatttcttcg cttattacat
    18001 gaacctttat taaattagta gttccactta ctcctactgg tactccagca gttataacta
    18061 ctaattcccc attatctata taatcagctt gtctagctgc ctcaattgcc ttttctataa
    18121 cttcatctgt atttcctgca acagaagact taactggata tactccccat gtaagagcta
    18181 atttattcat agtcttttca ttatttgtag tagctattat tggagatttt ggtctgaatt
    18241 tagataccat tctagcagta tatcctgaag aagtagatgt tattatagct gatgcattta
    18301 aatctactgc tgttgtacaa gttgcatggc ttattgcatc tgttacagta acattgttag
    18361 taccattttc ctttagtaat ctatcataat ctaaagtctc ttctgttctt tttgctattg
    18421 tagccatcat ttttactgcc tctacaggat attttccagc agcagtttct ccagataaca
    18481 ttatcgcatc tgttccatca tatatagcat tggcaacgtc tgtaacttct gctcttgttg
    18541 gtcttggatt tcttatcata gaatcaagca tttgagtggc agtaacaact ggctttgcta
    18601 attcattaca tttttttatc atcatttttt gaactattgg catctcttca gttggtatct
    18661 caacacctaa gtcacctcta gccaccatta taccatcaga aacttttaat atttcatcta
    18721 ggttctctac accttcttgg ttttctattt tagatattat ttgtatatca gttgcattat
    18781 tgttctctaa tacttctctt attgctagta catcagatgc ctttcttaca aaagaagcag
    18841 ctatataatc tattccttga cttattccaa actctatatc acttatatct ttaggagtta
    18901 ttgctggtaa atttatctta acaccaggta catttactcc tttatgattt ttaactattc
    18961 ctgaattttc aacaacacaa actatatctt ctccatttat ttcttttact cttaagccaa
    19021 ctaaaccatc atctattagt atagaatctc ctgatttaac gtcttcagca agacctttat
    19081 agcttactgt acacatttct ttagtcccca taatatcttt catagttata gtgaactttt
    19141 gcccttcttc taaaaatacc tctgggtctt caaagttacc tgttctaatt tctggacctt
    19201 tagtatctaa taatatagca actggcttat ttaatttttc tctaactttc tttgccatat
    19261 caattctttc tttatgttct tcatgcgaac cgtgagaaaa gttaaatcta cacacattta
    19321 gaccattttg cataagttga gttaaaactt cctcactttg tgatgctgga cctagtgtac
    19381 aaacaatttt tgtcttcttt acattattta acatatttgc tcctcctgag atattattcc
    19441 acagatttat ttttaatact aaaacttata tagataatat ttttgccatc tcataagctt
    19501 ctttatcaaa tactttcttt tgagctaaag cttcacttat ttctaaatca attattttgt
    19561 tttcttttat tcctacaact ctagctgatt ttccatctaa taataattct actgctctta
    19621 ctccaagtct acttgctagt attctatcag atacagttgg gcttcctcct ctttggacat
    19681 gccccaatac agtgactctt aaatctgctc ctgactcttt tttaagttcc ttttctaaat
    19741 cactagcact tccgactcct tctgctaaaa cgattatact atgtctctta cctctttttt
    19801 gagtagtctt taatcttaga gcaacttcat ctactgtaat ttctacttct ggtactatta
    19861 tagtttccgc tcctcctgca agacctgcat ataaagctaa atctccacag tgtcttccca
    19921 taacttctac tatgttaact ctttcatgag atgatgatgt atctcttatc ttacctatag
    19981 catctataat agtattcatt gcagtatcaa atcctatagt ataatctgta tatgctaaat
    20041 cgttatctat agttcctggt atgcctatag ctgggaatcc taagtcacta agtttttggg
    20101 ctcctgcaaa tgaaccatct ccaccaatta caactaagca atcaatttta tactttttaa
    20161 gaatttttac tgcttttaat ctaccttctt ctgttttaaa ttcttcacat ctggaagatt
    20221 ttaatatagt tcctcccctg tgtataatat ctccaacaga agataagttc atttcagtta
    20281 aatcttcttc tagtaatccc ttatatcctc tattgattcc atatactttg catccataat
    20341 atattgctga tcttacaacc gctctaatcg cagcattcat tcctggagca tctcctccac
    20401 ttgttagtaa tcctatagtt ttcatttttt acctccttgg attttatgtc aattttttat
    20461 tataatcatt gttattgtat tctaatggac tttttttgtc cctatatcac tttttaatcc
    20521 catcaagact aaaaaaatta aatgtcctta aatgtccatt ttgtgatatt atatcacaaa
    20581 acggacaata tttttcatat tattataata tatttagatt tttatataaa aaatcaagct
    20641 tttactttta tgttatcttt aggaagtaac tcttctaagt tatttataaa ttcatcacat
    20701 atacaagtca atgccccggt cattttatat gctttacgtt taccatttat attttcatcc
    20761 atagggtata agtatatact gtcattacct ggatattgtt tggctatttc tacaatatta
    20821 ttagttgtat cagtatcaga catactatct attttcaaat acaatttttt accactttct
    20881 ggcttaatat tttctacatt tctagttctt cctgaattgt agtttctatc tgcaaagtca
    20941 gtatcatcat ttatatcttt tatctctcta gctataagtt ttgcattttc gccttctttt
    21001 atacttaatg ttccttttac atatattata ttatcttcat tcaaaatgac attatacttt
    21061 tgtaataatt gaggaaaaac tattatttct atagcaccat acaaatcttc taattcaata
    21121 aatgccataa tttcatttcg ttttgttgtt ttaatagttt tagttacaat cattccaccc
    21181 attataacat ctgtttcatt catttgtagg tatgactctt cttcctcttt taatgaattt
    21241 aattttccat tatccataga tgttttttct tgtagttctt tttcaaattg tgataaagga
    21301 tgtccactta catacattcc caagacttct ttttcaaggt taagtctctc tctttcttcg
    21361 tactcttgcc ttttaggcaa actgtatatt tgttgaaaat cactatcttc ctcactatct
    21421 ccaaaagcat caaataaaga aatttgacct tgaacatttt tctttctatc catagataca
    21481 ctttctaata aactttcata accagccatc agactagctc tattttcact tatattatca
    21541 aaagctccac attttattag actttctatt actctcttat ttgtatcttt ttggtctaat
    21601 cttttgacta aatctactaa atctacaaat aatccattag attctctttc agcgattata
    21661 ttgtttatta tatttacgcc aacatttttt acggctgcta gaccaaatct tatattgtcg
    21721 ccttcaactg aaaactttga aaaacttttg tttatatctg gaggtaatac atctatttct
    21781 agtgctttac attctcttat atactcaact accttatcag tatttcccat tacactggtt
    21841 atcaatgctg ccataaattc aactggataa tatgttttta gataagctgt ctcatatgct
    21901 aaaaccccat atgcagctgc atgtgattta ttaaatgcat atctagcgaa gtctatcata
    21961 tcatcaaata ttttattagc tatttcctct tctacaccat ttctgataca ccctgcaatt
    22021 tctatattac catcttcatc aaatttacca tgaataaaat attgtctttc ttcttccatc
    22081 acgtccatct ttttcttact catggctctt ctaactaggt cacttcgtcc gtaactataa
    22141 ccacctaaat ctctaacgac ctgcataacc tgctcttgat ataccaaaca accatatgta
    22201 acttccatta taggctttaa tttttcatgt agatatgtta catcttttgg gttgctttta
    22261 ttttttatat atgcaggtat agaatccatt ggacctggtc taaataggga tattcccgct
    22321 acaatatctt caaaattgtc tggttttagc tgtttcataa aacttctcat gccagcactc
    22381 tccaattgaa acacccctag tgtatttcct gatgaaagca tttcatatac tttcgggtcg
    22441 tcataatcca tctttgaaaa gtctattttt tctgtgtaat tttttataac tctatttttt
    22501 tctattaaat caagagtatc tcttataact gtaagagttc ttagcccaag aaaatccatc
    22561 tttaaaagcc caagttcttc aagtgttgtc atagtaaact gtgttgtgat tgcatcttga
    22621 tgtttgtaca aaggtacata ttcatctacg ggattttttg atataacaac tcctgctgca
    22681 tgagtagaag catgtcttag cattccctct atctgttttg atatatttat tatttctttt
    22741 gtttccgggt cttggtcata caattcttta agattaggat ttgtatctag ggccttatct
    22801 attgtcattc ctaaagcaaa tggtatttcc ttggctacct tgtcaacttt attgtagcct
    22861 atatccaata cccttccaac atctcttatt gcagcttttg cacccattgt tccaaaagtt
    22921 attatctggg ctacatggtc atcaccatat tttctcttta cataatctat aacttcttct
    22981 cttctctcat aacaaaaatc tatatctata tctggcatgg atatacgttc tggatttaaa
    23041 aaacgctcaa ataatagaga gtactttatt gggtctatat cagttatttt aagtgtatat
    23101 gctactatcg aaccagccgc actacctcta cctggtccta ctataatatt attttcttta
    23161 gaaaaattta taaaatccca aacaattaaa aaatactcta cataacccat tttttcaatt
    23221 acatctaatt cataatttaa tctctctagt atctcattac taggattatc atatctctct
    23281 tttaatccat taaaacacaa ctcccttaaa taagtttctg gtgtatatcc ttctggaaca
    23341 tcatacttag gaagatgtat agtattaaaa tcaaactcca cattacacat atcagctatt
    23401 ttcacagtat tgtcaatagc ctctaatgca tatggaaata attcttccat ttcttctctt
    23461 gattttaaat aaaactcatc actaccaaat ctcattctat ttgggtcatt taatgttttt
    23521 cccatttgaa tacacataag tacatcatgt attttcgaat cttctttatt tacatagtga
    23581 acatcattag tagcaactag tggtatacca gtctcttttg ataattttac taaaccagag
    23641 tttacttctt tttgctcagg taaattgtga tcttgaatct ccaaaaagaa attttcttct
    23701 ccaaatatgt ctctgtagtc aagagcaaat tgttttgctt tttcataatt tctattcatt
    23761 aaagcacgtg ctacatctcc tgcaaggcat gcagaaagtg ctataatccc tttgttatgt
    23821 tttttaagtt cttccatgtc aactctaggc ttatagtaaa aaccttctac gtatgaagtt
    23881 gaaactattt ttattaaatt tttatatcct tccatattct tagctaacag aactaaatgt
    23941 ccttgatatt tgtcatagtt tgggtcttta tctctcaatc cacgtgcagc tgtatagact
    24001 tcacacccta taattggctt tattccttct tttatagctg ttttgtagaa atctataaca
    24061 ccaaacatac aaccatggtc agttatagct attgagctca tgtttagttc cttagccctt
    24121 tttataagtt ttttcaccct cgaaaaccca tcaagtaaac tatactcagt atgtacatga
    24181 agatgagcaa aatctttgtt cattaaatca cctcctgctt tataatttta attttaccac
    24241 aaatatatgt cctaaaacaa aaacagagac ttttatctca aaataggaat taacttcaac
    24301 atataccatt atatgttata aattaatgct tattctgaga ttgtcccctc aatttattgc
    24361 tatgattttt ttttcaaatc tgttgcaatt tcttctattt ttctaagtct atgattgact
    24421 ccagattttc ctattggtgg ttctaacatc tctccaagtt ctttgagagt catatcctca
    24481 tactcgattc taatcttagc tatctcttgt aaattttctg gcaaactgct aattcctata
    24541 gtctcttgta tgagcttaat attttctact tgtcttactg ctgcatttac tgttttagaa
    24601 aggttagctg tctcacaatt tactatcctg tttacattat ttctcatttc ttttactatt
    24661 ttagtatttt gaagacttaa aagagcgtgg tgtcctccta taatactcaa caaatctgat
    24721 atttgttcac tctctttgat ataaacaacg tgattatttt ttctagccac aattttacta
    24781 ttaaaaccta aagagtttat aagttctttt agagaatctg caaactcctc attattagtt
    24841 acaaattcca tatgatagtt cttttctggg tcgcttatag aacctccacc aagaaaagct
    24901 cctcttatga aagctctctt acatccttca tgcttaatta agttctctga aattttgttt
    24961 atagtaaaaa atccttcttt atcttcaagt atacctaact tttttaataa catttctgaa
    25021 cccatttcgc ttgttaccat taatacataa ctgttatttc tctttagcat ttgattctta
    25081 tttactgaga ttgtagtatt gatattaaaa ttctttttta ataatttaaa tactttacgg
    25141 gctattgaat ttaactctgt tgttatcttt agatttagct tcttatatcc aacaatttgt
    25201 atacttcccc ctgatttaac tagagctgac aattcagcta tattacaaca ttcattccca
    25261 gatataactc ttgctagttc atttttggtt tcagttgaaa aagacatatc tatcacctat
    25321 taactaattt ttctttaacc ttttttttat ttcttgtaag gcaaccttta ttatctcctt
    25381 ttcatcatca tacctagcta agtctactac tctatctagg tggataaatt tagcatcaat
    25441 aaagccttcc tctttttgtg gaatagtatc catattcttt gcttgcatca aataccaaaa
    25501 aactgtttta tgaactgctc tattttcatc ccagttttct ttgaatgtat agtgtatttc
    25561 acctaaatat ttcaatatat ctgcttttac tccagtttct tcacttactt cacgtaatgc
    25621 tgcctcttgg ttattttccc cttcttctac ttttcccttg ggtaacaccc aatctccatt
    25681 gaacttcctt agtaaaagaa tcgtattgcc aaacaggact acaccaccag cactaacctc
    25741 ttctctcatc gatgttcact ccttaaaact aacataatga taaggatttc tacacaattt
    25801 tttaaaatcc ttttttacta acttttgttg taattatgat ttagggctaa ctcaataact
    25861 atgttagata tatactttgc atcatgtctt atataattat tttttatttc aattaaattc
    25921 ttctctacag tttttatacc catctctttt aatttatctt tttgcttttt atctaacaag
    25981 acttgctctg ctccatcttg tttatataag tcaagcattt cttctggcaa tatctcatta
    26041 tttgcgatta cataatctat taaattctca tcagtgtgtt ttactatagc attaacatgc
    26101 tctaatacat tgtacccttc tgtctcacct ggttgagtca ttatatttgc tatatatact
    26161 tttggagcta cagacgattt aatagcgtct acaatcccct ctacaagtaa attgggtatt
    26221 atacttgtat ataaacttcc aggacccata attataatgt cagcatcata aatagatgca
    26281 ataacatcct ttaatggttt tacatctttt ggatttaaaa aaattttatc aatagagctt
    26341 ttttgaattt ttgactcttc aggtatacta gattctccat ttattatatt gccattttct
    26401 aatttggcaa ctaaatttac atcttccaaa gttactggta aaactcttcc tgttatagca
    26461 aaaatttcac ttaatttata tacagctttt tcaaagtttc catacaaccc attcatagca
    26521 gccaaaaaca aatttccaaa actctgacct tttaaaagcc cttctgtaaa cctatattgc
    26581 ataacttcat tcattgttgg ctctatattt gcaagtgcta ataaacagtt tcttatgtct
    26641 cctggtggga gcattcctaa gtcttctctc aatacacctg aacctccacc atcatcagca
    26701 acagttacaa ttgcagttat attttgcgta gtgtgtttaa gacctcttaa aaataccgat
    26761 tgaccagtac ctccacctat cacaaccaca tttggttctc tattttcaac aatagacctt
    26821 tgaaatagtt ttactttttc attttttgct tttttatata taaataacga tacaattaat
    26881 gcaatccaac ctaaaattcc tacaataata agtaatttag ccatgtttgc ctccattatc
    26941 gcaacataga gtctctatgt ttctttacaa ctctatatcc cttattagat aaatcctccg
    27001 ctataagatt tgttatagta actgacctat gtcttccacc tgtgcatcca acaccaatta
    27061 ctagatgttg ttttccttcc tctatatatt gaggtactaa aaaatgaatc atatctaaaa
    27121 gtttcacata aaattcttca ctgattttag aattcataac ataatctcta acttctttat
    27181 catcaccagt tttagctctt aactcttcaa cataataggg atttggaaga aatcttacat
    27241 caaatactaa atctgcatca gcaagtatac catgtttaaa tccaaaagat actacagaaa
    27301 tagttaggtt tggattatct tcacctgaag aatatatttt ctttatttct tccttaaggt
    27361 ctttaggttt catgttagtt gtatcaatta tacatgtaga taactccttt aaaggttcca
    27421 ttatctttct ttccattttt ataccttctg gaatctgcat atcttttgcc aatggatgat
    27481 ttcttcttgt cattttatat ctctttaaca atacatcatc attacaatcc aaatatacca
    27541 tttcatattc ataattttct ttttctaaat aatttaagct ttcatgaagc gcttcaaaaa
    27601 actttctccc tcttatatct atccctagag caactttatc aatacttgaa tttggttgat
    27661 aacataattc tgcaaattta gttatcaaag caggaggtag attatctaca caataaaatc
    27721 ccatatcctc taaagctctc attgtctcac tttttccaga tcctgaaaga cctgtaacta
    27781 taacaaattt cattgctaac tcctcccttt tatcttatgt ttattatatt ataatgtttt
    27841 ctactaacga tatatccaga ttagagtcag atatagtctt catagctagt tcaaaatcac
    27901 caactctttc tggacaatgt gcatctgagc ctattataaa tttattttct agccctttta
    27961 ttttatttaa ttgatttaca ttcaaaaaag aatggctact atttatctca agtctagtat
    28021 ttgtcttttt tgctgtcttt gctacttctt ctatatacac atcgccttta tctcctggat
    28081 gagtaattat aaaaatatca ttattactta tcgcatttat tacagctttt gtattatatt
    28141 cttttgcttt ttcagtttta aaaatatagt tattacaatg atttaataag cttcttaatg
    28201 atgttggagt agaaccaaaa tgatatccag ccataatata atcaaaatct ctaataaatt
    28261 tatcatccat atctatattt cctttatcat ctaatatgtt acactccata cctaaaagta
    28321 tatttatatc agtgtactta tcatttaaat aatctacttc ttctctcatt ttgagaatat
    28381 cttttttctt tacaccatat gcaatatgtt tataactatg gtcagatatt cctattgttt
    28441 ttattccttt agaaatggct acctttacat tatcctcaat agtccctttg ccatgactat
    28501 ataatgtatg tgtatgataa tcagccagta ttttcatttg ttaatcctct ccaagtattt
    28561 tgacttcttc ttccaacata accccaaact tggcatatac agcacttttt ataacatata
    28621 ttaaatccaa aatatcttta gcacttgctt ctccttgatt gactacaaat ccacaatgct
    28681 tctcagatac ttgtgctcct cttaaagtaa gacctctcaa tccagtttct tctattaatt
    28741 tacctgcaaa atatcctgtt ggtctcttaa atgtacttcc tgcactagca aaatttaatg
    28801 gctgtttagt tattcttctc gtcgctaaat catccattaa actttttatt tcatcataat
    28861 ttccttcttg taattccaat tctgctgata aagctatata tccgtttttc gatattatac
    28921 tttttctata cccaaattcc atctgttcat ttgataattc aaatatattg ccttccatat
    28981 ccatcagtct tactgacttc actatgtcct tcatttcacc accatatgct ccagcattca
    29041 tggctaatgc tcctcccaat gtgccaggta ttcctgatgc aaactcaaac ccttttaatt
    29101 cttgcttttg gagagctttc cctaaaactg ataataaagc tcctgactga gcagtcatcc
    29161 tagtatcatt tatctcataa gaattgaaat tatcagctag ttctattacc actcctctta
    29221 ttccaccatc tttaattaaa atgtttgaac catttccaat tattaaatac ggaatagatt
    29281 cttcttttac caacttaagt acatttttta attgttcttc agttctaggt ctaactaata
    29341 tatctgctgg tcctcctacc ctaaatgaaa tatgtttttt cataggttca tcgactttta
    29401 tatcctcttt acttaatata tttaataaat ttatatatat atcttgatta ttcattgtat
    29461 ttcctcccaa attatactga actttagctg tcattattat ttaaattaca ttagtatata
    29521 gtattaacta tattcaataa tatatgattt tgcttatcat atggatttta tattttaagc
    29581 ttcttaatta caaactatat tcaataaata tataataaag taaaaaaaaa aaaataacaa
    29641 catataaaag aatataaata atttaaaaaa agcatcaata tcactgatat caatgcttta
    29701 aaaggcaatt taattcgtat tatttatcta actatttttg acatagcaaa gataataaat
    29761 ccctccaact actatggaac ctccaacgat atttccaagc gttacaaata ccaaattagt
    29821 tataaattga accatagtta tatttgctcc taatgccatt cccattggta taaaaaacat
    29881 gtttgcaaca ctatgttgga atccacataa tacaaatagc attattactg tccaacatga
    29941 agccaatttt gacattatat cttgagatgc tgtagccatc catacagcta aaacgactag
    30001 aatattacaa agtattgctt ttaaaaacat tgatgctata gttgaattaa ccttagcttc
    30061 tgccacagct atagcttttg tagctgcatc tcctgtcaat gtagaagaat aaaacacaag
    30121 tgcaactagc aaaattgaac ctactaaatt tgcaaaataa accagtcccc agtttcttaa
    30181 catttttcct aatgttattt ttttgtccat aagtgcaata gccattaagg tgtttccagt
    30241 gaataattct gctcctgcca ctactacaag cataagacct actggaaaga ccatagcact
    30301 taaaaagcta gctacccctg ggtctatctt gccaaaagtt tgactaacta tcatactccc
    30361 caatgcccca aaacctataa atgctcctcc aaataaacct aatagaattg cgttttgtgt
    30421 cgaaatattt gcttttttaa tgcctgcact tatagttgct tgtgcagttt ccccaggtgt
    30481 caagaatcct ttactcataa atccttcccc cttaaaaatt atactttccc ttgtcttttt
    30541 atggcaataa ttatataata acaatatgat atataataaa ttttattaac ataaaaaata
    30601 tactatatac ttatatat
//
LOCUS       DAMJVI010000037        30362 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.039, whole genome shotgun sequence.
ACCESSION   DAMJVI010000037 DAMJVI010000000
VERSION     DAMJVI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30362
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.039"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            108..1172
                     /locus_tag="PC545_001790"
     CDS             108..1172
                     /locus_tag="PC545_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HDF2456561.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEINLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            1330..2016
                     /locus_tag="PC545_001791"
     CDS             1330..2016
                     /locus_tag="PC545_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456562.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSALKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINIFKQKIKAFLNHLTIKTKQDYYKDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKSILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLKYIKTVYGMG
                     YMWLGERNEF"
     gene            2006..3037
                     /locus_tag="PC545_001792"
     CDS             2006..3037
                     /locus_tag="PC545_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2456563.1"
                     /translation="MNFKKFSFSSIYLIIAVGIIFTAILFSISIYLITGDLKLIALLL
                     IYTMVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQK
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTSSNGKIRVATECWVMYTKIDII
                     DNGKGISESRQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSDIGKGS
                     TFSIFLPNK"
     gene            3119..3784
                     /locus_tag="PC545_001793"
     CDS             3119..3784
                     /locus_tag="PC545_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456564.1"
                     /translation="MSILQVINLKKYYGQSSNITKALDGVTLSIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMSDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDIPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            3797..6214
                     /locus_tag="PC545_001794"
     CDS             3797..6214
                     /locus_tag="PC545_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456565.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKAKNILYKNKNFSGVGTYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGKLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIVGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESRPDTVDVLV
                     KLSEENTEKDSASIKTQLNKIGLSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMIADLFIILILSLVMFLTVRISLFKPARIISKISAIEAMQYTE
                     TKFRKKAKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYEHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPYIKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     YYGLNLKVGDTVTFNIFSGNDIIKKKMKIIGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEVEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLRNMLYREGLNISIKAICTSST
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(6395..7186)
                     /locus_tag="PC545_001795"
     CDS             complement(6395..7186)
                     /locus_tag="PC545_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2456566.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYIDKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFHKNTQSNENISITQGNAMDLSV
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFAAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSAFNVTRLHYVASDGL
                     ALYMREAVDSMDDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(7219..7806)
                     /locus_tag="PC545_001796"
     CDS             complement(7219..7806)
                     /locus_tag="PC545_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456567.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNQYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGIAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            complement(7983..9374)
                     /locus_tag="PC545_001797"
     CDS             complement(7983..9374)
                     /locus_tag="PC545_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HDF2456568.1"
                     /translation="MERLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFN
                     PKGRFDSYPISITVGILVACFVMFSERFDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSVMLFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIFSPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSVFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCNTGVEKGLIGLMGGVIAAKGGTWGLAVGFILYFILIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            complement(9564..10862)
                     /locus_tag="PC545_001798"
     CDS             complement(9564..10862)
                     /locus_tag="PC545_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase/BAAT N-terminal
                     domain-containing protein"
                     /protein_id="HDF2456569.1"
                     /translation="MKVNVYNVESFVDERLKIMVSDLIPNTKLKISVKMEFPWCKGEE
                     FSSYGVFCSNEKGEVDLDLVEPIEGTYKADNCMGLIYSLEKSKTEGKNFAENISIDKP
                     IIMNMIFETSIERKEVELKRIFKSEDITTKYISDEFIGKLFYKQNSNNKTILMLGGSD
                     GNLDALDLLAAPLASRGFNVLTVAYFALEGLPDKLEEVPLEYFEKVFEWISENEITST
                     KEIFVHGTSKGGELAFLLASRYEQIKKVVVSQPHIYCFQALNGLMSGNDTSSWSYKGE
                     PFPYIKVDNDIFFEEQKKNISKDIPFGFNSTYKKSLKRAENRKEARIKIENSKADILM
                     IAGKEDNIWNSYEACLEALEIFEENNYPYNVELLTYNNMGYPLPIPYIMPLKETLCMS
                     MSRGIFSSGGTVEGNSKGQFESWNRTIEFYKKPLSSDLNN"
     gene            complement(11219..12094)
                     /locus_tag="PC545_001799"
     CDS             complement(11219..12094)
                     /locus_tag="PC545_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDF2456570.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLN
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTIKKMSNYLYNDCFKIHYAVVDALIENRITA
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(12287..12973)
                     /locus_tag="PC545_001800"
     CDS             complement(12287..12973)
                     /locus_tag="PC545_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDF2456571.1"
                     /translation="MFGVIANSLAIAGGCIVGLIIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(13022..13555)
                     /locus_tag="PC545_001801"
     CDS             complement(13022..13555)
                     /locus_tag="PC545_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2456572.1"
                     /translation="MSDLNNKKEQDENESSMNETKIYDILDKLNVEYDVVEHEAVYTA
                     EQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFASP
                     ERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVIKEDYLNFHPNINTKTVNISRKG
                     FEEFLEYLKYPIQSIDV"
     gene            complement(13599..14231)
                     /locus_tag="PC545_001802"
     CDS             complement(13599..14231)
                     /locus_tag="PC545_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDF2456573.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNENIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(14321..15400)
                     /locus_tag="PC545_001803"
     CDS             complement(14321..15400)
                     /locus_tag="PC545_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HDF2456574.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLENDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLRFIKNI"
     gene            complement(15533..16879)
                     /gene="pepV"
                     /locus_tag="PC545_001804"
     CDS             complement(15533..16879)
                     /gene="pepV"
                     /locus_tag="PC545_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDF2456575.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGQGEKMIGILGHVDVVPEGDLESWNYPPF
                     EAVLEDGKLYGRGTQDDKGPTISAIYAVKALMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDNEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTLLVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKATQIYALSIYELLKI"
     gene            complement(16948..18228)
                     /locus_tag="PC545_001805"
     CDS             complement(16948..18228)
                     /locus_tag="PC545_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HDF2456576.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEEIKDKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(18355..18615)
                     /gene="hfq"
                     /locus_tag="PC545_001806"
     CDS             complement(18355..18615)
                     /gene="hfq"
                     /locus_tag="PC545_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDF2456577.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNNR"
     gene            complement(18729..19664)
                     /gene="miaA"
                     /locus_tag="PC545_001807"
     CDS             complement(18729..19664)
                     /gene="miaA"
                     /locus_tag="PC545_001807"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDF2456578.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEEEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIHEINEKEKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQKQLAEKGIDYMHNKLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSSDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMIKNG
                     LVEEAKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLDQYENIDELKNEIILYIKDSIK"
     gene            complement(19702..21669)
                     /gene="mutL"
                     /locus_tag="PC545_001808"
     CDS             complement(19702..21669)
                     /gene="mutL"
                     /locus_tag="PC545_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDF2456579.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGIGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIKDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNNDLLESTPKNSNITKGKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASVEDDVKNINYLSEDSANDNIQEEFQVDGIKNEGNYYLGDSIK
                     DSEEEYLCSSKRKFSLYGYFVIGVVFNTYIILSKDDSMYLLDQHAAHERILYERYMEK
                     FYRQDINMQILLDPVVIEVSNIDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIF
                     GVPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLE
                     KCENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(21680..24523)
                     /gene="mutS"
                     /locus_tag="PC545_001809"
     CDS             complement(21680..24523)
                     /gene="mutS"
                     /locus_tag="PC545_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDF2456580.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSEMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAESADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKALNDEVALMKQNDDKEKINQEDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(24684..25418)
                     /locus_tag="PC545_001810"
     CDS             complement(24684..25418)
                     /locus_tag="PC545_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456581.1"
                     /translation="MLTQMNFYHNKKVKVSIENVSKTYKDIEVLKDISIDVYEGEFVS
                     ILGPSGCGKSTIFNIITKLTDYDSGKVNINGKYSYMYQKDLLLPYKTIIDNVSLPLIL
                     KKEKKSKARSMVKPYFEVFGLSGYEDKYPSELSGGMRQRANFLRTFINSNDIMLLDEP
                     FGALDSLTKTSMQKWLLDVKKKVNSTILLITHDIDEAIMLSNRIYVISKKPSIVKKEF
                     VIDSRKLNEDNLENIIKLKKEIISLL"
     gene            complement(25387..26400)
                     /locus_tag="PC545_001811"
     CDS             complement(25387..26400)
                     /locus_tag="PC545_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2456582.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKDTSSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVATVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKNNPELAKKFLKATTKGYEYAIKNPEESAKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPQ"
     gene            complement(26422..27192)
                     /locus_tag="PC545_001812"
     CDS             complement(26422..27192)
                     /locus_tag="PC545_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456583.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSMVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSIHAVVTIGEAILGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MDSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQAVKKVVIRHRLID"
     gene            complement(27167..27457)
                     /locus_tag="PC545_001813"
     CDS             complement(27167..27457)
                     /locus_tag="PC545_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HDF2456584.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(27588..27687)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     regulatory      28307..28396
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            28449..28625
                     /locus_tag="PC545_001814"
     CDS             28449..28625
                     /locus_tag="PC545_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456585.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(28740..29096)
                     /locus_tag="PC545_001815"
     CDS             complement(28740..29096)
                     /locus_tag="PC545_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456586.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLERDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            29352..30265
                     /locus_tag="PC545_001816"
                     /pseudo
     CDS             29352..30265
                     /locus_tag="PC545_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
BASE COUNT        10315 a         4799 c         3485 g        11763 t
ORIGIN      
        1 caatttaatt acgcttaaat tgaaaatata actttattct tatataatta aattatacaa
       61 caataaagtt atatttttta aatatatttt tactaggggg aatagaattg aaaagggttg
      121 atgagagaaa tactatgttt gcaagagcaa attacaaaaa agatagtact gcttataatg
      181 actattataa gaaaaatcct gacaaaaaag aaattgatga tagtataaga aatagaccaa
      241 atttatgtag tgaaggaaca atgacttaca atgagttaaa ctctccaatg gcaagtagtg
      301 cattcgattt tttatctgat atcaaatctt tatgcgaagg aaaagtttct gatactaaag
      361 tagatgtaga tgcaaagtca atgacaaaaa aaattaaggg tcttgcaaag cagtatggag
      421 cttctgtagt tggtataact aaattaaaag actatcatat ttatacaaat aggggtagac
      481 atgaagaaaa ttacggtgaa gaaattaatc taactcataa gtatgcaatt gtatttggat
      541 gtgagatgga taaagaaatg ataaatagag ctccaatgat atgtgaagtt atagaaacat
      601 caaagtgcta tgtggatgca tctatcgtcg gcatgatttt aagttactat ataagaaatt
      661 taggatatga tgctagaaat catatggatg ctaattattt agtaatgcct gtttttatag
      721 ctcgtgatgc tggtttagga gatataggaa gaaatgctat acttacaaat aaagattatg
      781 gctctagact tagattaggt gttgttacta cagatattcc tcttttagaa gatgaatatg
      841 ttgattttgg actagaagat ttttgtaagg tctgtaaaaa atgctctttt aactgccctt
      901 ctcattcgct ttctaatgat attaaaattg gtgatgatgg aaaatataat tgggtaattg
      961 aacatgagac atgctatatt aaatggagat atttaggtac agattgtggt atgtgtatat
     1021 ctgcttgccc atttagccaa aatctcgaaa ctataaagaa tactacttca tttaagggta
     1081 ataatgagct tattcaaaaa gctttagatg agtatacttc aaaatttggt aaaagaatat
     1141 ttatacctgg aaatccatct tggttgaaat aattttattt aataaagaat ctgtactaga
     1201 atttatcata ggacaggttc tttattaaat atataaattt ttaaatactt tttctcgttt
     1261 ataacaattc agcaatgttt ttagtctata attgacatta taaaatatta aactaaaagg
     1321 agtaactgta tggaaaaaat attagtagta gaagatgatt ctatattaaa taaaactcta
     1381 tcatataacc taattgaaga tggatatata attacttcaa aatttacagc taaatctgca
     1441 ctaaagtcta tttttgaatg tgaatttgat ttaataattt tggatattaa tttaccagat
     1501 aaaagtgggt ttgaattatg taatgaaata aaggaaaatt acaatatacc aataattttt
     1561 ttaactgcta atgatatgga atgtgatatg attaaaggct atgaacttgg agctttggat
     1621 tatataacaa aaccatttaa tatcaatata tttaaacaaa aaattaaagc ttttttaaat
     1681 catcttacaa taaaaacaaa acaagattac tataaggatg gatatcttga aattaacttt
     1741 tctgagttat ctgccaatat aaatggaaat caaattatat ttacaccatt agaatatcgt
     1801 actttaaaac tgcttactga aaatcctaaa agtattttaa caaggaaagt acttttagaa
     1861 aaactctggg atatagatgc taactttgta gatgaacata ctttaacatc tgttataagt
     1921 aggattagaa gcaaaattga aaaagacaat cttaagtata tcaaaacggt atatggtatg
     1981 ggatatatgt ggttaggtga aagaaatgaa ttttaaaaaa ttttctttta gttctattta
     2041 tttaataatt gctgttggta taatttttac agctatttta tttagtatct caatttatct
     2101 aataactggt gatttaaaac taattgctct attattaata tatacaatgg tactttgtgg
     2161 atttatagca ttaataatat ttttacttca taaaaaaata gttatatttt gttctaaaat
     2221 atgtcacact ttagatagta tgatgaatag tgaagataat attgaaattg aattagaaga
     2281 agaaactatt ttatcacgta tcaatcacag attaattaga atgtatgaag ttattcaaga
     2341 aaatcgcaat agtattgcta tagagaaagc tgatttacaa gaactagttt ctgatatatc
     2401 tcatcaaatt aaaactccta ttgcaaattt aaaaatgatt aatactactc tactaaatca
     2461 aacatttaaa acagaaattc aaaaggattt tttaatggat atggaaaacc aattagataa
     2521 attagatttt ttaatgcaat ctatggtaaa aacctccaga ctagagacag gtataatcac
     2581 actatctaaa aagaaaaatt caatttatga aacaattgct acagcactta gtggaatttt
     2641 gtttgatgct gaaaaaaaga atattgaagt tacagttgat tgtgacccaa atttatatat
     2701 aattcatgac aagaaatgga cttctgaagc tatttttaat atattagata atgctgtaaa
     2761 atacacaagt tctaatggta agatacgtgt agctacagaa tgttgggtta tgtatacaaa
     2821 aatagatatt attgataatg gaaaaggaat atcagaaagt cgccaagcag aaatctttaa
     2881 aagattttat cgtgaagaag atgtccatga catagaaggt attggaatag gtctctatct
     2941 ttcaagaaaa attatatctc ttcaaggtgg ctatattaaa gttacatcag atattggaaa
     3001 agggtcgact ttttctattt tcttacctaa taaataaatt tttaaaaatt tcatttgtaa
     3061 tgattctgca acattttggt tttatactgt atttacaaga tgttaaggag gtattattat
     3121 gagtatttta caagtaatta atttaaagaa atattatgga caaagttcaa acattacaaa
     3181 agctcttgat ggtgtaactc tatctattga aaaaggcgaa tttatttcta ttgtaggtac
     3241 atctggtagt ggcaaatcca cattattaaa tatgatggga ggattagata atcctacatc
     3301 tggaaaggtt attataaagg gtaaggaatt atctactatg tctgatgaac agcttacaat
     3361 ctttagacgc agaaatatag gttttatatt tcaaaattat aatcttgttc ctttacttaa
     3421 tgtatatgaa aatattatct taccaatagg attggatgga gagaccatag ataaggattt
     3481 tataaatgaa attataaata tgctttctct tgaagaaaaa ttatatgata taccaagtaa
     3541 tctttctggt ggtcaacagc aacgtgttgc tatagctaga gcacttgtaa caaaaccagc
     3601 tattatacta gctgatgaac caacaggtaa ccttgatagt agaactagtt cagatgtact
     3661 gagtttactt aaagtgacaa gtaaaaaatt caaccaaaca attgtaatga ttacacacaa
     3721 taatgaaatt gcacaactaa ctgatagaat tattcgtatt gaagatggaa aaattgttaa
     3781 ctaggaggtg tagtatatgt ttagaaataa taatcgcaca tttataagaa aaatagcttt
     3841 aaatgattta aaaattaata aacttaaaac atatctttca ggtatcatta taatgatttc
     3901 tacatgttta ttattaacag tgaccttagt ttcatataat gcctctgtag atttagttaa
     3961 tgcatctcca tatcatgcta tatataaatc tgtagatgaa aaagcaaaaa atatactata
     4021 taaaaataaa aactttagtg gcgtaggaac ttacaaatta attggaagta ataaaaagac
     4081 agattgtatt atgtcgattg tttatgcaga tgatacagca attaaactta tgaattttca
     4141 accattgaaa ggcaaactac ctacaaataa aaatgaaatt gctatttcag aaaaatattt
     4201 acaggaattt ggtttagata aagatattgg agatagcatt aaattaaatt attatgatga
     4261 tattacgaat aaagaagttc aatgtgattt tattattgtt ggatttctag aaaattatta
     4321 tcaagataat gccaagcaat actatacagt cgtttcaaat gattatttta agaatatagc
     4381 ttctacacct ttacaaaata aaagtaatac ttttgacgag tcaagacctg atactgtaga
     4441 tgtattagtt aaactatcag aagaaaatac agagaaagat tcagcttcaa ttaagacaca
     4501 gttaaataaa attggtttat ctttaggtat taagaaatat aatatatact taaatgataa
     4561 ctatattgaa tcaaatttaa ttgatggtga gcagattatt acagttttag tagttggaat
     4621 agttgttctt ttttctagcg tttttgttat atatagtatt ttttatattt cagttgttaa
     4681 ttcagttcag atgtatgcta agttgaaatc gttaggtatg acttcttttc agttaaaaaa
     4741 aattatttct ctacaaggaa atattttgtc aattattttt attccattag gggttatagc
     4801 ttcttgtata attgcttaca taattcaacc attagcatgg caaatgatag ctgatttatt
     4861 tataatttta attttatcac tggtaatgtt tttaactgtt agaatttctt tatttaaacc
     4921 agctagaatt atttctaaaa tatctgctat tgaagccatg caatatacag aaactaaatt
     4981 tagaaaaaaa gcaaaaaatt ttagttatat aaatataaaa aatctagcat taaagaatat
     5041 cgagtcaaat agaaagaaaa atttgattgc attaatatcg ctaagcatta gtggagtttt
     5101 atttatttca atcgctaatc ttgctaattc aatagatttc aaaaaacaac tatctcaaca
     5161 atttgtctat aacgaagatt atataattag cattaagagt gataacttat atgagcatat
     5221 tacagaaatc caacaaaata atccactaac agattctctt aagaatgaaa ttaaatctat
     5281 tcatggagta aaaaaattaa ttgaatccaa atcaattaaa agtaatatta tagaacctta
     5341 catcaaagat agagacgaaa aacaatttat tactctaatt aaaggtatta cgcctgaact
     5401 ttcaaaatca cttgaagaat atattgtcag tggaaaaata gactatacac atttagattc
     5461 agatagttta attataaata aatatagaac aaaatattat ggattaaatt taaaagttgg
     5521 agatacagtt acatttaata tctttagtgg aaatgatatt attaaaaaga aaatgaaaat
     5581 tataggaatc attgacaatt ccaatacagg aggtatgttt tttgcttcag acaaaacttt
     5641 agaatctcta acaccatata atacaaattt agatttttct attttaatag ataaaaagca
     5701 ttctaaagaa gttgaagaaa agttgttaag tataattcag aaaaattcaa atttaatgct
     5761 ttattcatat gaagatgatt atagaatgat tactagagca tttcaatata taattatagc
     5821 ttcatacatt tttgttggtt taatttcttg ctttggtata ctaaatatga ccaataccct
     5881 aataaacagc gttttaatta gaaaaaaaga gtttgcatta cttcaagccg ttggaatgac
     5941 aagaaagcaa ctgcgaaata tgctttatag agaaggtcta aacataagta tcaaagctat
     6001 atgtacttca agtacactag gttattttgg ttcaaatcta ctttgcactt ttataaaaga
     6061 tgtaattaga ttagacttta ttaattttaa attttctata tttactattt taattttttc
     6121 attcgtatta attggtatac aagttttagt gactgaatta cttgtaagaa atatagaaaa
     6181 aaaatcactt acagaacgct tacgttctca gtaagaatgt atataaaaaa tagccatttt
     6241 ttatacaagt tttaatatga ttttttaaaa gctataggga tagaaaattt tgactttaca
     6301 taattttcta tccctatact tgtttttatt ttatacagat acacttactt atatctccga
     6361 gcaacatcac cagagacata aacaagcata ttccttattt tctaaatatg tcaattgcat
     6421 gacttgtaac ccctactaaa tcttcacgtt cacaagtagc aaggtgatat tttaaatata
     6481 atgcaaatgc atcatcatcc atagagtcta ctgcttcacg catatataaa gccaaaccat
     6541 ctgatgcgac ataatgcagt ctagttacat taaatgcaga catcaaatca tcaatgtttt
     6601 ctttacgtac aagctcaaac aaatcctttg gttcagactt agcagcaaat gtctgtggat
     6661 caatcaaacc tttttcaata tattcagata cattaatatt tcctctatgg aacccctcgt
     6721 caatgagaca gccatcagat ataacatatg cagcaaaaac cacaccacca ggttttgtta
     6781 cacgaattgc ctcatgtaaa gcttgttgtt tatcttcttt gttataaaga tgatatagtg
     6841 gtcctaacag taacgtaata tcatatttat tatctgggaa aacagataaa tccatagcat
     6901 taccctgagt aatgcttata ttttcatttg attgagtatt tttatggaaa acctctatat
     6961 tatgttcgac taattccact gcgtcaacat catatccctg acgtgccagt gtgtgagaat
     7021 aacgaccagt tgcagcgcca atttcaagca cacaatcacc tgattttatg tacttatcaa
     7081 tgtaatgcat agtagtgaga aattccacca ttccatgttt taatgcaagt cgactatctt
     7141 catcataagt attataaaag tcaattaagt attgttttgt ttccataatt tcacctcttc
     7201 tatattagtt aataaacatc aaatagacaa attccaatct ggtttttctt gtcttataaa
     7261 agctgttaat atatttttca acattttttc agcactatat ctatctaatg ttaatttttt
     7321 tgttgctgtt aagacagcta tgccatgagt ataaagaaat aaatctaaaa atattacttt
     7381 tgcacattcc aactctattc caatgctttc cgaaaaaagt cttgcctttt tttcattgtc
     7441 aatttcttta taaaaatcat ttgcctcttc catcatcaaa tctaaatcat ttataaataa
     7501 caacttaaat aaatgagttt cttcttgagc aaattcaatg tatgaaagcg gaagcagtaa
     7561 ataaggactt acacttttag aattactata attaacaaca tactgattat agtattcata
     7621 tgcaaaagca agaaattcat tttttaattc attcatgttt tcgtaacaag taaaaattgg
     7681 tcgagtggaa cactgtaatt tacttgcaat actcctcgca tttacagctt caaatcccac
     7741 ttctcttgta atatccaaga ctgtattcaa aatcatttct tttgttatct ttactttagg
     7801 tggcatatat gttcacctct ccattaaatt tttataaaac acatatgtta tataactata
     7861 gtaacatatc aagaaattat gtcaagaaaa agctttgcat ttttttgcaa ttcctaaaac
     7921 ttataaataa aacatgagtt gcaattaatc tattaataga taacagcaac tcagattttc
     7981 ctttaaatac tcttcttttt gtctaatatt tcatatacac aatcttctat tgaattaata
     8041 tctttttctt ttcttttttt agaatcaatt aaaatgaaat acagtataaa acctacagca
     8101 agtccccatg ttccgccttt agcagcaatt acacctccca taagtccaat caatcccttc
     8161 tcaacacctg tattacatag ctccattgat agctttgtac ataaatatcc ttgaactaaa
     8221 agtgttactg acaatgctaa tggtataaga ggtgtagcaa tctgcgcaat tggataaaaa
     8281 cagataaaaa ttgctgaaac ccatactaat gtacccatgc cactaaaaac agactgcatg
     8341 gcttcctttc caaccttgta cctttgagca acacttcctg taagtgtcgc agctaatgga
     8401 ccgcacattg gtatatatgg agaaaagatt gccataaaaa tatttcttat cccacttata
     8461 acattagacc tatttgaatt gaaatctata tattcatctt ttctattttc actttcagaa
     8521 agtactaagt tttcacctgt tataaaatca ccaaaggcaa ttatgtatat cataaaagcc
     8581 attggtatcc cttgtataaa taacattaca cttggaaaac ctattccaaa aggagatact
     8641 gtatttatca gatttttaaa atctactatg taaactaaat tgtcaaaact aaatctagga
     8701 atttctattt ctttactaaa tatacctact attaaactca ctaaaatagc tgatactaca
     8761 ccatattctc ctaaatgaaa taaaaattta ttcttaactt ttagagtatc aaatctttca
     8821 gaaaacatta caaaacaagc aaccaaaatt cctactgtta tagatatagg ataagaatca
     8881 aatctccctt taggattaaa ctctgatatt atagtcgata taccgacacc cattagtatt
     8941 ccacctttaa tagaatttgg tatcttacta actaactttc cagctacacc agttataccc
     9001 attaaaataa atattattcc taaatctatt tgtaaagctg ttaatgcttg tattctttca
     9061 atgcccatgc tgaactttaa caaatatgca gttgtaagag ttatagaagc tgtaatccag
     9121 ccaggtacaa ctgggtctcc cataaaaaca tgcaaacagt ataatattga gttaatgata
     9181 gccatactcc atgccacttc atagggtaat cccaaaattt gtgtactaag gccaattgtg
     9241 cctaaacttg tcatacttga tattatacct tgaatagctt ctggtttctc aaatctgtaa
     9301 tgtataaatg gaattcttat cttaaacatt ccaaatttta tatgaggttg ttcccttcta
     9361 gcgagtcttt ccataacttc actccacctt tcaataactc ttataagttt acaactttat
     9421 aattgtttat aaattatcaa tgtcattaaa attagtatat aacatgcaaa ttatataaac
     9481 aataaattaa atctataaaa ttgatagatt ttgtgcatat atagaaagac cgcttcattt
     9541 ttagttactt aaatcactta atattagtta tttaaatcac ttgataaagg ttttttataa
     9601 aattcaatag ttctattcca tgattcaaac tgtccttttg aatttccttc tacagttcca
     9661 cctgaagaaa aaatccctct actcatactc atacataaag tctcctttag aggcataata
     9721 tatggaattg gtaacggata acccatatta ttgtatgtta ataattctac gttatatgga
     9781 taattatttt cttcaaagat ttctaatgcc tctaaacaag cttcataact attccatatg
     9841 ttatcttctt ttccagcaat cataagtata tctgctttgg aattttctat ttttattcta
     9901 gcctcttttc tattctctgc tcttttaaga cttttcttat aagtactatt aaatccaaat
     9961 ggaatatctt ttgatatatt ttttttctgt tcttcaaaga atatatcatt gtctactttg
    10021 atatatggaa atggctctcc tttatatgac catgatgaag tatcatttcc actcatcaat
    10081 ccattaagag cttggaaaca ataaatgtga ggttgtgata ctacaacctt tttaatttgt
    10141 tcatatcttg aagcaagtaa aaaggccaat tctccaccct ttgatgtccc atgtacaaat
    10201 atttctttag tactggtaat ttcattttca ctaatccact caaatacttt ttcaaaatat
    10261 tctaatggaa cctcttctaa tttatctggt aacccttcta aagcaaagta tgcaactgtt
    10321 agtacattga atcctctaga tgcaagtgga gctgctaaaa ggtctagagc gtctaaattt
    10381 ccatcagaac cacctagcat taaaattgtt ttattattag aattttgttt ataaaataat
    10441 tttcctataa actcatcaga tatgtatttt gtggtaatat cttctgattt aaatattctc
    10501 tttaattcaa cttcttttct ttcaatagaa gtttcaaata tcatattcat aattattggt
    10561 ttatcaatag atatattttc tgcaaaattt ttaccttcag ttttagattt ttccagcgaa
    10621 taaattaacc ccatacaatt atcagcctta taagtacctt ctataggttc aactaaatct
    10681 aaatctactt ctcctttttc atttgaacaa aacactccat aagatgaaaa ttcttctccc
    10741 ttacaccatg gaaattccat tttcacactt attttaagtt tagtatttgg aattaagtct
    10801 gaaaccataa tcttaagtct ttcatccaca aatgattcta cattgtatac atttactttc
    10861 ataatctatt tctccttata ataattattt atgttttata taaaacagct ttatctaata
    10921 caagataaat aaatttttaa attaagacac catattgact atttcatttt atcaacatat
    10981 ttcatctttg tcaacatatt gtctttttta gtaatttaaa attatggtct atatgaaatc
    11041 cagttgattt ttggctaatt aaagatagga atgctattag gtttagaata tatatattca
    11101 aaataaaaaa ccactcattt aaagaatggt ttttaatata aactatttgt tattaataag
    11161 gatatttttt tatttaaatt tatataatcg aacatctaat atttaatgtt taataaaatt
    11221 atgcatttgc aaaagtatat atggcattaa ctcttgctac atataaattc tcatcactac
    11281 atccaatata tctagcatat ttttcaatct tttcttttgc attttcaaca tcattttcat
    11341 cgctctccat taactcattt atataaatat ccattaatga cgctttatat tctcttatat
    11401 catagcctaa aaattcagtc aatctctcaa tttccttact aaaatctcca tcttctttta
    11461 ccaaaaattc gtatacatct tccctatatg gatttaaagt tattatttgt tttactactt
    11521 tgcattcgtc agccttccca ataaaaccat ctttataatt attaaataag gcattagatt
    11581 tttttataga ttcttcatca atataagaat gtaccgcagt tatacgattt tctataaggg
    11641 catctacaac agcataatga attttaaaac aatcattgta taaataattg ctcattttct
    11701 ttattgtcga ttctatttct aagaagctcg cgtggccatt ttttttattt ttatttggta
    11761 taataaaagt tttattcaaa ttattaaaaa gtctcttaaa atctaaatat ttttcacaat
    11821 aaacactttt aaaggtatcg atatctatag ttataattcc aaaacttact atagtctcaa
    11881 ttcctttctt aaaagactct tctatatatt tttctcctaa acttaaacct ctatcagata
    11941 tttgctttag attttgaaaa ctatttaaac aatctctttc aaagttttta gatatttcta
    12001 aagcatataa tttaaagatt ttccttatct tattataatt attttttgta tcatcaaatc
    12061 tgataatagc tccatttaca tcaaaagtaa tcattgagtt ttaacctccc cattagtttc
    12121 atcaataaaa taaatttaat ataaacttta taatcataat attaaactta tttatactat
    12181 aattattaat ttgatgataa aagctatttt ccttctaaat aaaaaatttg tctaaagttt
    12241 ctaaaaaatt tagacaaatc ttaggttatt actctttaaa ttatttttaa attattccaa
    12301 atacgccaaa taatattggt ataaatatag ctggtaacaa attggctact tttatattcg
    12361 ttaatttaag tacatttaga ccaagtccta taattaataa acttccagct gctgtcattt
    12421 ctgtgactac aggatcactt aaaaatccat ttaaaaatga tgctcccaca catataatac
    12481 cttcatacaa aaatacagat attgcagaaa acattacacc tatacctaaa gaagcagtaa
    12541 atataattga agctattcca tctataacag attttacaaa taaagtatca tgacttcctg
    12601 taagaccact ttctaatgaa ccaactactg ccatggctcc tatacagaat aataaactag
    12661 atgatataaa accctctgct atagaatctt ttttatttcc tttagaaaat ttactttgta
    12721 aaaaagcacc caacttattt acccatttgt caatatctat taattctcct atcagagcac
    12781 ctacagctac ggatattatt gtcactaatg tgttttttcc ttctagtgct cctgatatac
    12841 caatgtacaa tacacataaa gcaattccat tcattatagt atcgcttact ctttgaggta
    12901 atcctccttt tattataaga cctactatac atccacctgc aattgctaga ctatttgcta
    12961 ttactccaaa cattttttct cccctcgatt agctaattga aatttatcct attattaaaa
    13021 cctaaacatc tatactttgt attggatatt ttaaatattc caaaaattct tcaaaacctt
    13081 ttctagatat atttacagtt ttagtattta tgttaggatg aaaatttaag taatcttctt
    13141 taattacatc tttatctatg taaagctcaa cttttctttc tgtatcattt attaatgaaa
    13201 aaggatttac acttccagga agaagtttaa gtacattgta tagtctttca ggtgaagcaa
    13261 acgataatct agaacttcct atcttatctt taagtgagtt taaatcaact tgtttttctc
    13321 ctttaactac aataagataa tacttatttc ctttggcatt tcttaaaaat aaatttttac
    13381 attgacaccc ctttgttatt ccatctaaaa tgactaattg ctctgctgta tatacagctt
    13441 catgctctac tacatcatat tcaacattta atttatccaa aatatcatat attttagttt
    13501 cattcatgct agattcattt tcatcttgtt cttttttatt atttaaatca ctcatacttt
    13561 tctccttaaa cacttcatca taatatcatt gtgaggtatt attctactat agctattgct
    13621 cttactggag ctccatcagc atcattatac ttaaatggtg ctgctataaa tagaaatttt
    13681 tctggaacat tttctaaatt tgtaagattt tctatgatta tttttccttt ttcaaataaa
    13741 atatgatgca cttcaaatac tgatgtgtca tatctatcca ctgatagcat atctattcct
    13801 atgcctttta tattagtatt agctatatag tttgcagctt cttttgttaa tgtaggataa
    13861 cctacatagt actgcttttt atcccaaaat tttgaccaac ctgatttaaa tattataaaa
    13921 tcactatttt ttatttcatc ttcatatttt tttagatatt ctaattctat attctcattt
    13981 cccttttcaa gttccaatac ataagctttt ccaacaaaat tttctatatc caacttatct
    14041 agggtctcac ctttagtata catatgtctt ggtgaatcca tatgagttcc attgtgagaa
    14101 aatacactaa ttaatgtttc ttgataacca ttttcttcaa taatagctac tttcttgata
    14161 tctggtctat taggctctgc atatacaggc atgtcattat gtgtcacatg agttaaatca
    14221 aaaaccttca tctttatata ccccctatgt ttttattttc tattatatac ctaaattcat
    14281 tttatttcta tcagatttta ttccattata taccatacta ctatatattt tttataaacc
    14341 tcaagggatt taccgaaaaa tcttggaaag aattatcagt aaatataaca tcttcaccca
    14401 aaaaacgttt ataccttcct tcttctttct ttaaagtaat tctctgtttt tgtgaatgat
    14461 tattaaaaac cattatctta agaggaggtt tttttctatt agtctgcact ctttttaaca
    14521 tgtattttat gtgcatatca gcttcaggaa atgaatatcc acaaaatatt aaaaggtcag
    14581 tatctcttaa agttttttct gtttcattcc aaacattact taaaaatatg tttgacatat
    14641 tcttaaaata tgttggtggc actataatag gttctgttaa ctcaccacac tctaaacatt
    14701 ttgtctctga actattttca ataagcttca tgacaccacc ttcatgtggt gtaagagtta
    14761 cactattaca aatggggcaa taaagccaat tcaatgaccc atgtatctta tataatttga
    14821 ttaatggttc ttttggtttt ctccaattta actcctcttt aaaatttgta aactccacac
    14881 cataatcaag cataattggc aaatcattct tatctcttaa tccagtaact gtattgtcta
    14941 tatgtatatc ataatttgca ctaataaaag tagtatcaag aagcaaatca ttttccaaaa
    15001 gattttcaac taaaagttta tgatatttat tatctgttct aggtgtatta tgaattgctt
    15061 cagccattag cagtattaaa tattgtctta aaaaacgtat actatcactt ctgttattta
    15121 gattttctag tccaaaattt ctaaatgctt cttttctttg ttctgctaag tctaaaagac
    15181 ctaaaacttc ttcaaaggta ggaaaatttg cattatctat attatctttt actatatcaa
    15241 tattgaacat ctgtttaaag aaaatataca attctctatt cattttggta ttataatttg
    15301 tgggatttag ttttttaaaa tattcgctaa aaagttcatt ttgaataggt agattctctg
    15361 ctgctgaagc tcctgctcct aaaaatattg ctattttcat atagttcctc ctaatataaa
    15421 tattactcct atttttatat ccattaaata tccatatcat ttattttatg atacaacaat
    15481 aaagctgtct tttactagaa aaaatcctat tacaagacag ccttatatta tattaaatct
    15541 ttaataattc gtaaatagaa agagcatata tttgagtagc ttttataata tcctctacta
    15601 ttaagtattc attagcttgg tgttctgttt ctggttttcc tggaaaaata gctccaaatg
    15661 caacacaatt atctaaagct cttgcgtatg ttgctcctcc agatgaaagt ggtgttccat
    15721 caagaccagt ttcttcttca tatacttttc taagtgtctt aacaagaaga gtatcttctg
    15781 gaacatagat tgaatccaag aaatcatatt cttcataatt taaattgtat ttatctgtca
    15841 ttttctttaa ctcttttaca aaatcatcct ttttgtatgt tactggaatc cttacatcta
    15901 ttcctgcaaa ttctttttcg ttatcaatag ttactctacc tatatttact gttagtttac
    15961 ctgatacatc atccttgcaa ttaggaatta tattgtttcc attagcatct tcaccaataa
    16021 cttctgctaa aaacttaatt atattagaat ctatacctat attattaaga gctatacaaa
    16081 gtctagccac tgcatttata ccagtatcac ttgctgctga atggacactt tttccaatta
    16141 tacagatttt attaccttca acagtatact caaaatttaa tttatcaagt tcttttttta
    16201 atttatcaga gtattttcca atatagattg ctttacctgg aactgcattt aaagcctttc
    16261 caactgacaa ctctatatca cttttgctat cacaagtcaa atgaacttgt aaaagacctt
    16321 tctctgcatt tgttattggg aatctagaat ctggagtaaa tccatagttt ggtatttctt
    16381 cattattttc tttatattta tttatacatc tccaaagatt ttcttcatca gccccaaaaa
    16441 tgaatcttat cctcttatta aagtctacat ttaaatccat aagagctttc actgcataaa
    16501 ttgctgatat tgtaggtcct ttatcatctt gtgttcctct tccatacaat ttaccatctt
    16561 ctaaaacagc ttcaaatgga gggtaattcc agctttctaa atcaccttct ggaaccacat
    16621 caacgtgacc aagtatacct atcatttttt caccttgtcc tatttctgca tatccataat
    16681 atccatcttt gtatactgtc ttaaatccta aagatttaca taagtctaat atacctctta
    16741 aagctttatc tacattttca ccaaatgggc aattttcagt tgcttcagat ataacactag
    16801 gtatttttac actttcttgt attgaagaaa tcatttcatc ttgtagagaa tttacttttt
    16861 ctttaatttg ctctttcata ttatcaccct tcaattttca tcataatata ctaataaata
    16921 ttttgacctt ttaaaaaaaa aatatgctta ttttttcagt ttttttataa attcaatcgc
    16981 ttttaaagtc cccatttttg aatggtcaaa tgtaagtcca ccttgaaaat aaacattata
    17041 aggaggtctt attggagcat ctgcacttaa ttctatagaa gaaccttgta taaatgctcc
    17101 tgctgccatt atgacttcac tttcgtatcc aggcatgtcc caaggaacag gttttacata
    17161 agaatcgact ggtgcagctt cttgtatacc ttcacaaaaa cttattactt catcagcatt
    17221 attaagtcta atacattgaa taatatcact tcttaagtca tcatattttg gtaatacatc
    17281 atatcctaat ttttcaaaag ctcttgagca aaagatagct cccattacag cctgagatac
    17341 tatataaggc gccaagaaaa aaccctgaag tacatttcta gttgttccaa aagtaagacc
    17401 acattctttt cctattccag gagaagtcat tctataagat ataagctcaa tcaaatcttt
    17461 tctaccagct atatatccac ctgtcaatgc aagcccacca ccaggatttt ttattaaaga
    17521 gccagccata acatctgctc caacatcagt aggctctttg gtatctaaaa actcaccata
    17581 acaattatca accattacta tagcttctgg tttaacagac tttattactt ctatagcttc
    17641 ttttatatca ctaattgaaa gtgattttct ccaagaatat ccttttgacc tttgtatcat
    17701 aacgagttta gttctatcat ttattttgtt tttaattcct tctaaatcta agtttccatc
    17761 ctctaaaaaa tctacatcat catatgtaac accatactct ttcaatgaac ctttttcttc
    17821 tcttatgcct ataactccct ctaaagtatc atatggtcta ccagtaacag ataaaatttc
    17881 atctcctggt ctaactatac cttgtataca taaagtaagt gcatgtgtac catttacaat
    17941 tattggtctt acaagagcat cttctgtatt aaatacttta gaataaattt cctctatttt
    18001 ttcacgacca atatcattat atccatatcc tgtagtccag ttaaagtgca tatcacttag
    18061 tttagattct tgcatagctt ttaaaacttt atattgatta tattctctta tttcttttat
    18121 ttcttcaaac ttatctttta tttcttccat tatttcttgc gatagtttaa aagtatcatc
    18181 atctattcca tagtagtctt ttaataattc ttttgtctca ttaagcattt tatcacctgt
    18241 ctctttatta ttttatttaa acatgtaaag gagactattt tactaaaaca aataaattat
    18301 agtttataag ttagtataag tctcctctca atcatcttaa attaaattaa ttatctatct
    18361 gttgttatta ttattgttgt tttggttttg atttgttaga ttaatatact ttccaggttg
    18421 tatagttgat acagcatgtt tataaatcat gttttgttgt ctattatccc cttctattaa
    18481 tattatataa ctatcaaacc cctttacaag ccctttaact tgcactccat taactaaata
    18541 tatagtaaca ggtatccttt ctttccttgc attatttaaa aacaaatctt gtaaatttaa
    18601 aactgtattt ttcattagat ttgtaccctc ccaagattaa attgaacttc taaaatattc
    18661 ctaccttata ttctatataa gttatgacaa attttcaata ttaagttgta tacttaaaaa
    18721 tttatatatt attttataga atctttaata tataagatta tttcattttt taattcgtct
    18781 atattttcat attggtctaa atcaaaccat tttgctgttt tatatctttt aaaccatgtt
    18841 atctgacgtt ttgcatacct tctagaatct cttttaatta tttcaatagc ttcttcgtat
    18901 gtatattctc catccagata ctttaatatc tctttataac ctataccttg cattgatatc
    18961 atatcttttt taaatcccat acttaaaagt ttttttgcct cttcaaccaa tccattttta
    19021 atcataatat caactctcat atttattctt tgatacaaat gttctctatc tctatttaga
    19081 actattatta ttggttgata ttcttcatta aattttaaat cacttgaaaa atcattcatt
    19141 ttcttaccac ttagacaaac ttcaagagct cttatgaccc ttttagtatt gtttttatgg
    19201 attctattag cagattcttc atcaagttct tttaacttat tatgcatgta atctattccc
    19261 ttttcagcaa gctgtttttg aagttcttct cttaattcat tattggcatc agattgtgca
    19321 aaatccatat tatatattaa agagtttaaa taaagtcctg tgcctccagt tactaaaaca
    19381 catttttcct tttcatttat ttcatgtata tatttttttg ctctttgttg aaactctgaa
    19441 actgaaaatg gatagtcagg cttaacttca tctataaggt aatgcttcac tccttgcatc
    19501 tcttcctctg taacttttgc acttccaata tccatatatt catatatttg catagaatca
    19561 gctgaaatta tctctgcatt catatctttt gctaatttta ttgacaaatc tgtttttcca
    19621 acagcagttg gacctgtaag tattataagt ggtatttttt tcataacatt cctttccata
    19681 tattttaaat atccaaatat tttacataat tcttttaaac attttttcta tttctgtctt
    19741 agatatttcg accattatag gtcttccatg aggacatgta aatggattct cacacttttc
    19801 tagctgttcc aataaacttt ttatttcaat atcgtatatt ttatcatttg cttttatagc
    19861 agacctacat gccatagatg caaatctctc tccttttaaa tcataattac ttgtaatttc
    19921 ttctatatta tcaattattt gaagtataaa tttctcagtt tcaggtactc caaatatagt
    19981 aggaacacat cttaccatta tatgattatt gccaaaaatt tctagctcaa aaccaaattt
    20041 cataaataac tccaagttat tttcaatttg aagcatatct atatttgata cttcaataac
    20101 cactgggtct aaaagtattt gcatatttat atcttgtcta tagaacttct ccatatatct
    20161 ttcatatagt attctttcat gtgcagcatg ttggtccaac aaatacatag aatcgtcttt
    20221 gctcaaaatt atataagtat taaatacaac tccaattaca aaatatccgt ataaagaaaa
    20281 cttgcgtttt gaactacaca agtattcttc ttcactgtcc ttaatactat ctcctaagta
    20341 ataatttcct tcatttttaa ttccatcaac ttgaaactct tcttgtatat tatcatttgc
    20401 actatcttca cttagataat taatgttttt tacatcatct tctacacttg catttaggac
    20461 ttcacttact gattgaaatt catttattgg tttttcactt gataaagtaa caacttcaat
    20521 gacttcatcc ttacctttag taatattact atttttagga gtagattcta ataaatcatt
    20581 atttcttaat ttataatctg tacttttttc tctactgtta atagcgattc taggttgtgt
    20641 ttttttatca ctataagtag catattttcc aataagattt gagtgtatta atttaacctt
    20701 taaaaaatct ctaagttcta tatacacttc ttgttctttt tcaaatttaa tttcaagttt
    20761 attggggtgt atatttacat ctatacatga tgggtctact tctatattta agaagcacac
    20821 tgcatgttta cctattggaa ttatagactt ataactttct gttattgcat ctatgattat
    20881 tttacttttt acaaatcttt tatttatata gatatgttgt aagtttttat ttgaacggta
    20941 tatattgtta ttacctatat atccattcat tttaaagtga ttgcacttaa actctacatc
    21001 tattatattt tccgttattt cttttccata tatacttcta atagtgttta caagttttcc
    21061 atcccctgga gtatttagca tctgtttatt gttatttgta tatttaaatt gaatgtttgg
    21121 attacctatt gcaagtttat ttatcaaatc acttatgttt atagtttctg catgagtaga
    21181 ttttaaaaat ttctgtcttg ctggtgtatt aaagaaaata tcttttataa tgatggtagt
    21241 tccattagta gaacctattg gttctttcga tattattttt ccaccttcaa catatatttt
    21301 agttcctatt atttcatctt tagttttagt cgtcatttct aattttgata cagctgaaat
    21361 agatgccaat gcttctcctc taaaaccaag agagtataaa tcatacaaat catcaatttt
    21421 tttaatttta ctagtagcat gtctcaaaaa tgatttttca acttcacttg aaggtattcc
    21481 tattccatta tctgtaatct taattagact ttttcctcca tcaattatat ctatggaaat
    21541 tttatttgca cctgcatcaa tggagttttc aataagttct tttactacag aagaaggtct
    21601 ttctacaact tcacctgctg ctatcttatt tatagtcaaa tcatctagga tgttaattat
    21661 atttttcatt tttacacctc taagatttta tttcttttgc ttttctttga agattataaa
    21721 gagagtttat agcatctaat ggagtcatat ttaaaatatc cagatttaaa atttcttctg
    21781 atagtatatc tctatttaca ctatcaaaag agatttgagt taaggctact tcttttacag
    21841 aatcttcttg attaattttc tctttatcat cattttgctt cattaaagca acttcatcat
    21901 ttaatgcttt aaatttagaa tttaatttat cataatcctt ttttatatgt ttgtagtctt
    21961 ttactataag ttcatgttct atttttagag attcatattt attcttaaaa ttactttgat
    22021 ttaccttaac taattcttcg tccaaattac tattaattat attattgttt tctttatctc
    22081 catttattgc aacactgttg taaacatgat ttttttccaa gtctttaagt atatattttg
    22141 ctctttctat gacttcgtct ggtaatttag ctaatttagc aacatatatt ccataactct
    22201 tgtctgctcc ctgaggaatt atttttctta aaaatattat tccttcccca tcttctttga
    22261 ctgctattga ataattttta acctctttaa actcttcttc taaatcagta agttcatgat
    22321 aatgtgttgc aaataatgtc ttacatctaa tatttttttg aatatattcc actattgacc
    22381 atgctaaact tataccatca taggtactag ttccacgacc tatttcatct aatataacta
    22441 aacttctctc tgttgcattt tttaatataa gggatacttc gttcatctct accataaaag
    22501 tagattgacc ttgagataaa tcgtcactag ctccaactct tgtaaatatt ctatctaata
    22561 ttggaatatc agcagactct gctggtacaa atgagcctat atgtgccata agagctatta
    22621 tagcagtttg cctcatatat gttgatttac ctgacatatt aggaccagtt attatgttaa
    22681 taatgttttc tccacgattt aaataagtat catttggaac gaaattttcc tctcctacta
    22741 tattttcgac aacaggatgt ctaccatttc ttatatcaag tttattattt tcatttattg
    22801 ctggttttac gtagttattt atatgtgcaa ctgttgcaag ggacacaaaa acatctatat
    22861 tagctatagt cttagccact ttttgtattc tatctatgtt tttatatatt gtatctctta
    22921 tctctacaaa tatctcgtat tctaaagatt taatcttttc ctctgcatgt agtatctttt
    22981 cttctatttc ctttaattct ggagttatat atctttctgc attactaagt gtttgctttc
    23041 ttatatatgt ctcatctagc tttgcctgtt taaagttggc ttttgttatt tctatataat
    23101 acccaaaaac tttattaaat cctattttta atgatttaac tcctgttttt tctctttccc
    23161 tgttttctat ttcctttaca agaaaggctc cattcttaga aatctctctt agttccttaa
    23221 gttcatcact aaaatctgat ttaattatgt ttccatcttt tatggttatc gtaggttctt
    23281 ctagaatagc ttcatctatt aaattgtata tatcatctaa tttatccatt tctgaaacat
    23341 actcttttag gatttttgca tttgataaat ttatagtatc ttttaaatta ggtaactttt
    23401 ctatcgaatt ttttaagtga attaactctt ttggtgttac tctctcaaat gctatctttc
    23461 cacatattct ttctatatcg tatatatttt taagtatatc atttaggtct tctcttaata
    23521 taaagtcatc ttttatttct tctattacat ctaatctatt ttcaatttta gatttattta
    23581 tcaaaggttc ttctacatat tttcttaaaa gtctacctcc cattgctgta gaagttttgt
    23641 caagtacatg taataatgaa ccttttttct tattccctct tatagtttga gtaagttcta
    23701 aattagttct agtaaacata tctagaacca tatattctga tgaattgtat atatttatat
    23761 tattaatgtt agatgttatt tgtttttgag tattgtatat ataattcaga agaattgata
    23821 agcttgactt tattagacct ttatcatcaa actttagttt ttgtagataa acatctggaa
    23881 aatattcttt taatatattt atatccaaat agttatcact aaaactttca tttatgtata
    23941 tattacttac agtagctata tctctaagct tttctataaa gtctaaatca tttataatta
    24001 tttcagttgg atggattttt gcaatttctt caattacctt atcttcattt aaacatgttg
    24061 cattagtttc tcctgtactt atatcaacat aagttagacc tatattagta ccatccttat
    24121 acaaagataa taaataatta ttctttttat tttcaagtaa atttccatct aaaactgtac
    24181 caggagtgat gacccttata acctctcttc taacaattcc ttttgctgta gatgggtctt
    24241 ccatttgttc acctattgct actttatatc cattttcaac taattttgat atatatgaat
    24301 tggctgagtg gaaaggaaca ccacacatag gagcacgttc ttctaaacca caagcttttc
    24361 ctgtcaatgc tatttcaaga gcttttgaag ctactaatgc atcttcaaaa aacatttcat
    24421 aaaaatctcc aagtctaaaa aacagtatac agtctttata tctatttttt acttcaagat
    24481 actgtttcat cataggagtt aatttattca tatctatttg cattatcaaa cctccttctt
    24541 ttctaattaa atattatatc atagtagatg attattatta acacaatata taaaattttg
    24601 acatataact aaaaacaatc caaaatgtat actcacattt tggattgcta agtttaatgc
    24661 ttattaaata aaaatatttt tttttacaat aaagatatta tttctttttt aagtttaatg
    24721 atattttcaa ggttatcttc atttaacttt ctactatcta taacaaattc tttctttact
    24781 attgatggtt tttttgaaat aacataaatt ctgtttgata gcattatagc ttcgtcaata
    24841 tcatgagtta taagaagtat tgttgaattt acttttttct taacatctaa aagccatttt
    24901 tgcatggatg ttttagttaa tgaatcaagt gccccaaaag gctcatctaa aagcattatg
    24961 tcattagaat ttataaaagt tctcaagaag ttagctctct gtctcattcc tccagaaagt
    25021 tctgaaggat atttatcttc atatccgctt aatccaaaca cctcaaaata aggttttacc
    25081 attgacctag ctttactctt tttttctttt tttaaaatta gtggtagtga cacattatct
    25141 attatagttt tatatggcaa gagtaaatct ttttgataca tataactata cttaccattt
    25201 atattcactt tgccactatc ataatctgta agtttagtta taatattaaa aatagtactt
    25261 tttccacatc cacttggccc taatatagaa acaaactcac cttcataaac atcaatactt
    25321 atatctttta aaacttcaat atctttataa gtttttgata cattttctat agatactttt
    25381 acttttttat tgtggtaaaa attcatttgt gtaagcatca cttgccttca tatccttatc
    25441 tattaattta tattctttta agaatgatgt atagttgttc catacactat ctttcatttc
    25501 tccccatcgt ggtgcatcat ctttgtactt gcttgctaaa tattcttgac tctttaaagc
    25561 tagtttttca tctacttcag gtgcatgttt tactaaaatt tttgctgatt cttctggatt
    25621 ttttattgca tattcataac cttttgttgt agcttttaag aattttttag caagctctgg
    25681 attattttta agcacagttt cattagaaat tataagtggt gtataataat ccaatctctc
    25741 atctaaatct tttactggta taaagtttaa gtcatatcca attaaatcag ctttaactgc
    25801 atcccaacct tcaaatatcc atgcaaaatc tacagttttc atagctgcaa agaaatcatc
    25861 ttgaccagta ttaactatct ttaacttatt aaaatcagct ccatttttct tcataactgc
    25921 tttaaaaaca gcttcttctg atggagagcc ccaaccacca taagtttttc cttcaaaatc
    25981 tttagctgtt gttatattct tttcttttgg tgatgcaaac ccagaagtat tatgttgtat
    26041 aacagttgct acagctttta ttggaagtgg atcttcacta gtctttgcat aagtaacttg
    26101 ttcttgatag cttataccaa agtcagcttt tcctgttgct actaaagttt ctgctccact
    26161 ttcaggtggt tgtactattt caacatccaa accttcttct ttatagtaac ccttatcaag
    26221 tgctacaaat aatccagtat gatttgtatt tggtgtccaa tcaagcacca tagttacttt
    26281 tttagaagaa gtatctttat cattgttttt gtctttattg ttatttgaag aacatccagt
    26341 tattgttgcc aatgtaaaaa cacttactaa acctaatatt cctaactttt taaaattcat
    26401 aattgcctcc ctcaaatacc tttaatctat taatctatgt cttataacaa ctttttttac
    26461 agcctgtaca attcctacaa acattaaact aaatataact actaatatag tagatgcaaa
    26521 tactttatca agtgcatatg cactttttga cctaagcata taaacaccaa gtcctttcgt
    26581 tccacctagc cattctgcaa cagttgctgc cattacagca tacgttgcag atattttaag
    26641 tccagaaaaa aatttatcca ttgccatagg aaatttaagg tgaatgaatg tattaacttt
    26701 atttgagtcc atagtcttaa ataaatttaa ataatcttta tcaatattct caatgccatc
    26761 tacaaaactt attaatatag gaaaaaaaca agtaagcatt accatcaata cttttggcat
    26821 tgttccaaat ccaaaccata tcataaagag tggtgctata attattgtag gtatcatctg
    26881 tgtaaccaac ataataggat aaagacattt ttttattatt gatacaaaat ccatcaatat
    26941 tccaattacc aatgatatta taattgcgac tataaaacca agtattgctt ctcctatagt
    27001 aaccacagca tgtatggata aattttgata atctttaaaa aacacattaa ttatatctac
    27061 tggtgtgggt agtatatact gaggaacttc taaaactcct accatagatt gccaaagaat
    27121 aagtataaac aaaaatgttg ctattggata tatactatct ttgactttat ctatgatttc
    27181 taagtttttc atctatagtc agacctccag ctttttcatc tactctagct actgttatga
    27241 ctctttcaca tccaagatta aaagggattt tatgtacctt ttttaataat tcaaaaactt
    27301 catcaaaatc accttcaaca gtagtgctca ttgctcctat ttcacattta agaccacttt
    27361 tttcaatttc agcaatacaa gcatctacta ctttatacat ctcttcttca cctttgtaag
    27421 gtcttagtgg cattactgct atgtctgcga ttaccattta aatcactcct ttaaaaataa
    27481 ttaataccta ctaggtatat tttgtttttt attaaaaaat tttattacta aaaagatatt
    27541 atatttaaat aaaagaaaag tcttatccaa aataggataa gacttatttt tatcttaact
    27601 ggtctcccta cactggcatt atccaaacag gttcaaaggg tcgagataaa atctcctctc
    27661 agcacattag ctccccgaaa tatattatat cttttctact tgattatact acacttatca
    27721 tattttaaca aattttttat ttatattaga tattttatcc gttcaaaaat atctaatata
    27781 aattgtacat tattacacca aataaattat cttaaaatgg taatttatca acatcttcca
    27841 aaactagatt aactgaattt tctagttttt attttgctat ttcaagcata tttggatgta
    27901 tatcaattcc atacaaatat tgtgctaaat ttctgttttc actcatctaa tttgcaatcg
    27961 ttacaaatta cagattagaa atagctttta gactctactt atctttatct acaaaaaatc
    28021 cttcaataaa ttcattaata atattcagta atctaaaaat aaacctctat ctagattttt
    28081 aagatacaag ccttttagga agtatctttt gtgataatat aaaaattact catcattatc
    28141 atcaaaatcc tcagcaacca acattttaga taagaatcaa actctataat ataattattt
    28201 tatccaattt atttttgaga aataaaatat ttttttaatt tttaaatttt ttaatatgca
    28261 ccttgaaaaa agaaacgttt tcctatatta tggtaatagt agaataaaac taaataggca
    28321 aaactagaga aatttagtga cgcaaagcta tagggactaa gacttatgaa atgagttatg
    28381 tcagccagtt gccaaagaga tattttcttt ttgtttttat gaatgttttt tagggggata
    28441 tatgatatat gtttatggat aaaatggata gatgtacaca tatactaact gcatatatat
    28501 gcagttcacg cgattactgc aattttatag atacacaatt aaatgatttt atactagaat
    28561 atggagagaa tgtggtagaa tcttgtttgc atcaagtgat ggtattggta agcagatata
    28621 attaaagaaa ttttattttc tagaaaaccc tttttagttt tatctacgtt ttataattca
    28681 ttaaagaact aattaaaata agcttcatta tatatttttc ataaagtaaa cagttgagtt
    28741 tatctcatat actgtttacc ccaattacat aattctaata atatagattc taatgattta
    28801 ccacgtttag tagacaaata ttcaactttt ggaggaatag tcggataaga aatcctctta
    28861 ataagtccat ctctttcaag atactttaat tcttgtgaca gtatcttatg acttatatga
    28921 acaacttctt tttttaattg actgtaacgt attggctctt gttttaaaat atgccataaa
    28981 ataattgccc tccatttact tcctatcaca gtcatggtaa aaccaattga acaattaaaa
    29041 tctttttgtc tttcttcaat atttggaaat tcttcaaaat tattatctat atccatctca
    29101 atcctctctt tcaaaatagt aagtatctaa ctaaaaagtg cattcttgtt tagcgtacta
    29161 attcgtaata taatcttgat tgtagtatag agccttacat aatactactt ttttagatgt
    29221 atatgtttaa actttaatat agacatatgt tattttatta attttattta aacagataat
    29281 aataaataat aatttatctc aagttttcta tgtagataaa ctaatctaat gtttagaaag
    29341 gattatataa tatgaataaa aaatttcgtc tttatcaagt tgactcatta caaaagaaaa
    29401 atttacaggt aatccagctg gtgtaattac aaatgctgat ggcttatctg aatcacaaat
    29461 gcaaaaaata gcaagagaat taaataattc agaaactgct tttattttca ataacttggc
    29521 taaaaatcat caagaaaatt atgatgtaca tatcagattt tttacactaa ctaatgaggt
    29581 tcctatatgt ggtcatgcta ctattgctgc ccattatgtt agagcagtag aaaaccaatt
    29641 caataacaaa acagtatttc aaaaaactag tgctggaata ttaccagttg atattataaa
    29701 aaaagataat gattatgaaa taattatgac acaaggaaaa attgaagtat ctccaccgtt
    29761 acaacaaacc tatcaaaaaa gattacttaa tgcatttggt attttaaagg aagatattcg
    29821 tacagattgt cctattgcca tatcatctac aggacattca aaagttatga ttggaataaa
    29881 ggaattatca acattaaata gtttatctcc aaatttatat gaattatcga aaataagtca
    29941 agaaataaaa tgtaatggat attatatttt tacattacat aataaagaaa ctctacaagt
    30001 tcatggaaga atgtttgctc ctgcaattgg tatttcagaa gaccctgtta caggaaatgc
    30061 taatggaccc ttaggtgcgt accttgtaca ttataaagta atacctacac ccaacacaga
    30121 gtttatattt gaagcagttc aaggagaaac aataaaacgc aaaggcaaga tgttagtaaa
    30181 agtaattatt ttagataata aaccagttga agtaaaaata attggaaaag ctgtaattgc
    30241 attctcaaca aacatagaaa tctaattata gattccttct aagtaaattt ttgaactttt
    30301 taatactaat ttaataacaa actaatttat atacaatcaa attaattttt ttattatttg
    30361 tt
//
LOCUS       DAMJVI010000038        30052 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.040, whole genome shotgun sequence.
ACCESSION   DAMJVI010000038 DAMJVI010000000
VERSION     DAMJVI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30052
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.040"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            231..1310
                     /locus_tag="PC545_001817"
     CDS             231..1310
                     /locus_tag="PC545_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2456587.1"
                     /translation="MPELLKNMYNRESLYEVAVAIQSVYNSFKVDEFIKSTMDETWNN
                     LELKARCRKISMSLGMYLPEDYKEALSILEKSVTGFYFAFFFPDFVEVYGQDDINWDL
                     SISALERNTEYWSSEFAVRAFIIKDEERMMAQMRKWSKHKSEHVRRLASEGCRPQLPW
                     GQAISKFKKDPTPVLPILEQLKTDTSTYVQKSVANNLNDISKTHPDLVISIAKDWYGK
                     NKSTNWIVKHGCRTLLKKGNRDVLALFGYDDTTSINLQDFTLETTSISIGEDLTFSFN
                     ILAKKATKTRLEYGIDYIKSNGKRNRKIFKISEVSLKENEKKSYMKKHSFADVSVRKH
                     YPGIHSIAIIINGIEKDKLDFELGI"
     gene            1634..2002
                     /locus_tag="PC545_001818"
     CDS             1634..2002
                     /locus_tag="PC545_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2456588.1"
                     /translation="MDVYSFWNDTLAQDAVKIRKYFHENAYINWHDTNEHFTLEEFII
                     ANCEYPDKWNGKVEKVLEFGNLVITVTHVYAINKPLSFHVVSFISLKDDKIISIDEYW
                     GEDNDVPQWRLDKHIGKPIK"
     gene            2119..2922
                     /locus_tag="PC545_001819"
     CDS             2119..2922
                     /locus_tag="PC545_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2456589.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKKVVLIGQSAGGF
                     VAQSFIVKYPNMVEGLMMIGTCPYGTDYYSKSDIFWLKQTEWMLKLFPDKFLRNIISK
                     MCGATSEGRRSMMKMLEGYEKKELCHLMYLGFAGFIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQLNI"
     gene            3307..4197
                     /locus_tag="PC545_001820"
     CDS             3307..4197
                     /locus_tag="PC545_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2456590.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKQAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDTKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVFKYQKTYGLNYVCTVQNKDCCEKLLREECARYGILTD
                     MKDIIKAYKRSESYTQIKFF"
     gene            4660..5103
                     /locus_tag="PC545_001821"
     CDS             4660..5103
                     /locus_tag="PC545_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2456591.1"
                     /translation="MIEIKTIEINKKSQIDLLILADPCEEMIDKYLNKGTMYALYDNK
                     ELTCIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMVVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
     gene            5275..5445
                     /locus_tag="PC545_001822"
     CDS             5275..5445
                     /locus_tag="PC545_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456592.1"
                     /translation="MDSNNNTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMS
                     WIYVLYYWVKYA"
     gene            5818..6939
                     /locus_tag="PC545_001823"
     CDS             5818..6939
                     /locus_tag="PC545_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2456593.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRGDFS
                     NEKLDKIKDLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKQFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEERNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RALDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGIEKGIEKGIEKGIAKGKR
                     EEKFNIIIKLKNKGYNLSEICDIIDDLNKSEVEKVYNQN"
     gene            7144..7632
                     /locus_tag="PC545_001824"
     CDS             7144..7632
                     /locus_tag="PC545_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HDF2456594.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLSNVKLIKEFV
                     AK"
     gene            7718..8125
                     /locus_tag="PC545_001825"
     CDS             7718..8125
                     /locus_tag="PC545_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456595.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKVLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            8126..8344
                     /locus_tag="PC545_001826"
     CDS             8126..8344
                     /locus_tag="PC545_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456596.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENNI"
     gene            9418..9713
                     /locus_tag="PC545_001827"
                     /pseudo
     CDS             9418..9713
                     /locus_tag="PC545_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10121..10810
                     /locus_tag="PC545_001828"
     CDS             10121..10810
                     /locus_tag="PC545_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456597.1"
                     /translation="MDILLVEDDKNIREMVYDFLISENYSVKTAIDGNTAIEIFKNYS
                     FDIILLDLMLPKSSGFEVLKYIRSVSVVPVIITTAKDSDTDKMLGLNLGADDYITKPF
                     SIVELLARIKANIRRATVYSSSRNSQDMVIRYKDLIINISKYVVSKNGIDLNLTHTEF
                     EIVKLLATNQGRAFSKEQLYNAIWKEPYYGNENVLNTHINRLRNKLSDGIEKNNYIKT
                     LWGIGYKMEEE"
     gene            10810..11727
                     /locus_tag="PC545_001829"
     CDS             10810..11727
                     /locus_tag="PC545_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2456598.1"
                     /translation="MELFLVVISIVLFALYLKERNKSKKMESDYKYINSRLRDITNDD
                     EINYILVPSDISIVKETAVDINKLVEKFYSRQINYNRSEKAILQIFTNISHDLRTPIT
                     VLKGYIEMLYLQSQKEELSSSMRAIVEKMQTNSNELVNSINNLFNMAKIQSGDMILNL
                     QKSNLTQLCHEIILEFYDLLDKENFNVEVNIEDRPIYANVDTEALQRILKNLIDNAIK
                     YGSDGRFLGISLYEKEKHVFIDIEDKGSGISETEKEHIFTRAYTADRKKGNGLGLAIS
                     QGLANSMGGSIYVNSRPNVKTVFTVKLKS"
     gene            11800..12726
                     /locus_tag="PC545_001830"
     CDS             11800..12726
                     /locus_tag="PC545_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456599.1"
                     /translation="MNYIIETHQLKKIYKDKAVVNAVNIHVKKGEIYGFVGPNGAGKS
                     TVMKMLLNLVNPNSGEIVMFGKKVAETDFEILKKIGTIIENPYLYENLTAKQNLDLHC
                     EYMGYYNKEHISEVLECVGLSKQSNKKVSKYSLGMKQRLAIARAILTKPELLILDEPI
                     NALDPEGIREMRELFRKLNTDYGITIFISSHILSEVEQIADTIGIIQDGKLIKEISMS
                     DIHKYQTDYVEVDVDNVELAGYLLEKEFGIKDFKITSESCIEIYDLRKDVKEISKIFI
                     QNQIGINSIGRKQSSLEDYFFQTTGTGGKETL"
     gene            12723..13403
                     /locus_tag="PC545_001831"
     CDS             12723..13403
                     /locus_tag="PC545_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456600.1"
                     /translation="MIHLMKLELKKVGLKRYFLFSMLGIFLCMFFVFTGLNDKSTSIY
                     DYDVTFRTISLFFCFYYIILFAVLNVAYIINEYTNKTILILFSYPLDRKKLILSKLLL
                     ITTLMISSMIVGYICCFTFVVVLDKYFNLVIGDFHISILSYWIPGAIKSIIVFCSLGI
                     WTFVVGMIRKSIPVTLVSSIIFINLRQFILAGTNTTQDSLISVFIIVGITVIGIYYIL
                     NHKVTEID"
     gene            13501..13836
                     /locus_tag="PC545_001832"
                     /pseudo
     CDS             13501..13836
                     /locus_tag="PC545_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427885.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            14940..15590
                     /locus_tag="PC545_001833"
     CDS             14940..15590
                     /locus_tag="PC545_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456601.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEEEEV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            15917..17983
                     /locus_tag="PC545_001834"
     CDS             15917..17983
                     /locus_tag="PC545_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2456602.1"
                     /translation="MKEVVESLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENGYTSQVYILNNKTGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKNGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLPEEEQQKKINAYNKRHYEHFKEIPYAVNGEGYISRKRKRLYVYDSNINELKAI
                     TAPMFNVVGMKISDGKILYIGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIIKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSGHQHHCIGNTVTSDVKMGA
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVGCL
                     GNGLPEVYTVENGTLCKKTHLNDHILEEYKISVPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLAIHGGPRLTYGPYFMHEIQVFTSAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            18000..19955
                     /locus_tag="PC545_001835"
     CDS             18000..19955
                     /locus_tag="PC545_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HDF2456603.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKSGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGVDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENITKAITPKFCEVAGVDVSSDKNRILYTGQIYEDCATPFS
                     GLWEYHIDSGETKELVPQGKYQISLAKYIGKDKVMLQASTLDRSITQNHDIFILDLST
                     NEINMIASPDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFNEFDTKTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFVAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDTCIEKYPGIDADRLGVA
                     GGSYGGYMTNWIIGHTDRFKAAVSQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIARANAIVDWFERYL"
     gene            19971..21161
                     /locus_tag="PC545_001836"
     CDS             19971..21161
                     /locus_tag="PC545_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2456604.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTAAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAENTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVIVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            22310..22684
                     /locus_tag="PC545_001837"
     CDS             22310..22684
                     /locus_tag="PC545_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456605.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAN
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            22686..23543
                     /locus_tag="PC545_001838"
     CDS             22686..23543
                     /locus_tag="PC545_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456606.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVVKRDSGKIKIFGKDFDGNEKELKNRIGVVFDDSYFYEELSMAEMKSIIAP
                     SYDNWCEEDYQQNMKRFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTGYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     DEHRKVKGDIQFLNDETKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            23546..24184
                     /locus_tag="PC545_001839"
     CDS             23546..24184
                     /locus_tag="PC545_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2456607.1"
                     /translation="MLIHLVKKDILIVKKYVLLILFIAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFAMVFIYCTLVY
                     WIENLLIPQIGTFDLTYILIVLLISSIIYGVYMPIQYKLGYETTKFFFVMIIMASSFV
                     LPLTIAHSNFEIPFIYQYPSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            25190..25924
                     /locus_tag="PC545_001840"
     CDS             25190..25924
                     /locus_tag="PC545_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2456608.1"
                     /translation="MNLAKGLDVLKISSNVFGEDKVMYIPVIYTEDDATLIDTGLPGQ
                     GDLIIDALNKSNTSFDKLKNIIITHHDIDHIGNINYLREKSKNNIKVYAYKSEISYIT
                     GEETPFKLYMLEQMVDKIDDKMLSMLNVMGLGFKSSYTKVDVSLDNHEKLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQVENGELKPVDVMYSNKQELKDAIKNISNYD
                     IETIVFSHGGLYQRNIIGTLKNLIIE"
     gene            26720..27976
                     /locus_tag="PC545_001841"
     CDS             26720..27976
                     /locus_tag="PC545_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2456609.1"
                     /translation="MIEKFKTLIWIKMIIRIKSVYKTYGGLFFLGLIGIPVGAIIGLI
                     DTIFGTVLLKVTDIRETYPMYLIPFLAVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLVPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWVGKRLPIKNASSIFLV
                     TGMAAGFAGLFETPIAAILFAMEVLVAGSLEYQSLFPAFTASFTASAVSKALGLEKFS
                     FALSSKVVFDLSIFWKLIVLGIIFGMVGGAFAWCLKLSKRKIGNRLKNPMIRIAIIGV
                     CLSVLFLLFYKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFILTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYIFNMDKSIYSLQKISTKQ"
     gene            28039..29232
                     /locus_tag="PC545_001842"
     CDS             28039..29232
                     /locus_tag="PC545_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2456610.1"
                     /translation="MLTSLALIFLLGMASGGIFKRIKLPNLLGMLLTGIILGPYVLNL
                     IDNSILDISSDLRKIALIIILTRAGLSLDINDLKKVGRPAVLMCFIPATFEIIGMIVL
                     APKLLGVSILEAAVMGAVVGAVSPAIIVPKMLKLMEEGYGTEKSIPQMLLAGTSIDDI
                     FVIVMFTVFTGLAQGNSISAISFLQIPVSIILGVIVGAVIGIYLAVFFKKVHMRDSAK
                     GVLLLSISFLMISLETALEGIVPFSGLLAVMSIGIFLQIKYRVVARRLSIKYSKLWVG
                     AEILLFVLVGATVDISYAFKAGIGAVILIFGVLLFRMVGVFFCLIKTNLTIKERLFCM
                     IGYIPKATVQAAIGGVPLAMGMASGQLILTLAVLAILITAPLGAFGIDVTYKKLLTSV
                     KGENK"
     gene            29433..29813
                     /locus_tag="PC545_001843"
     CDS             29433..29813
                     /locus_tag="PC545_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2456611.1"
                     /translation="MCLKKLSKLELVIMKFIWNLDIKTNSYEIIDYMKEEHNLPEKVA
                     LKTLSKLSKKRFLYVQETGKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEELTEEKIKSLENWVVNWEEAYV"
BASE COUNT        11433 a         3168 c         5175 g        10276 t
ORIGIN      
        1 cagtaaagta taaaattcta ttactataat aaaagataga actagaagtc tttatgaaag
       61 tgggaaaagt atttatataa ataaattcat atagtttata gtttaatagt aggaaatatc
      121 gtatgagtaa gaatattgta ttttacataa agatttagca atatgacaca ctgttgtcgt
      181 gttctgtagg atatgattaa taaatcagat atgaaaggag agtgagtaat atgccagaat
      241 tactaaaaaa tatgtataat cgtgagtcac tttatgaagt tgcagtagct atccaatcag
      301 tttacaactc atttaaggta gacgagttca taaaatctac aatggatgag acatggaata
      361 acttagaatt aaaagccaga tgtcgaaaga taagcatgag tttaggaatg tatttaccag
      421 aagactataa ggaagctctt tccattcttg aaaaatcagt tacaggtttt tattttgctt
      481 ttttctttcc agattttgtt gaagtttatg ggcaggacga cataaattgg gatttatcta
      541 taagtgcatt agaaagaaat actgagtatt ggtcttcaga atttgctgta agagcattta
      601 tcatcaagga tgaagaacgt atgatggcac agatgcgtaa atggtctaag cataagagcg
      661 aacatgtacg aagacttgca agtgaaggtt gtcgtcccca gttgccttgg ggacaagcaa
      721 tatccaaatt taaaaaagac cctacacctg tgctacctat attggagcaa cttaaaacag
      781 atacatcaac gtatgtacaa aagagtgttg ctaataatct aaatgatatc tcaaaaacac
      841 atccagattt agtcataagc attgcaaagg attggtacgg taaaaataaa tctactaact
      901 ggattgtaaa gcatggttgt agaactctac taaaaaaagg aaatcgtgat gtattagccc
      961 tttttggata tgatgatact acttctataa atcttcagga ctttacttta gaaacaacgt
     1021 ctatttctat tggagaggat ttaacttttt catttaatat tttagcaaaa aaagcgacaa
     1081 aaacaaggct cgagtacgga attgattata ttaaatctaa tggaaaaagg aataggaaga
     1141 tatttaaaat atcggaggtt tcattaaaag aaaatgagaa aaagtcatat atgaaaaaac
     1201 attcatttgc agatgtaagt gtaaggaaac attatccagg tatacactcg attgctatta
     1261 ttattaatgg tatagaaaaa gataaacttg attttgaatt aggtatataa ttttactaac
     1321 agaataatct atatcagata ctttgctaaa cttatataat atattttact aaatttatat
     1381 taggtagttt actacttgtt tactgaattt aaatacaaag aaatacaggt gctaatccct
     1441 gcccattaaa ggaatatgag cataacacaa aagtagaact tatcaattct gttacgggta
     1501 tagtagtaaa tccatagaat cacaattaga agatgaagtg taaaaaataa gttagtgttg
     1561 attttagttg ctagtgatat tgaatagtta tacaatagct atataaatta gaatttgtag
     1621 aggagaaatg taaatggatg tatatagttt ttggaatgac actttagcac aagatgctgt
     1681 taagataaga aaatattttc atgaaaatgc ttatattaat tggcatgata caaatgagca
     1741 ttttacttta gaagaattta ttattgcaaa ttgtgagtat cctgataaat ggaatggtaa
     1801 agtagaaaag gtgctagaat ttggaaattt agtaattaca gtgacacatg tttatgcaat
     1861 aaataaacca ctatcatttc atgtcgtttc atttatcagc cttaaagatg ataagattat
     1921 ttctattgat gaatattggg gagaggataa tgatgtgcca cagtggagat tagataaaca
     1981 tattggaaag ccaataaaat agtttttgtt ctgttttata actgaaattt tgataacatt
     2041 tgatattttg atttgtcatt ggtcaacata gtcgttagtg taataattta aaaaagtaga
     2101 atttggagag tgaacactat ggaagaaaaa tacatcttat ctgaccgagg gaaagtatat
     2161 tattggataa aagaaagtaa tagtgtgttt gcaaagaact taattttttt gccaggacta
     2221 acagcaaatc atcatctttt tgatttccaa atacagtact tttcaaaaaa tcataatgta
     2281 ttggtatggg acgctcctgc tcatggaaaa tcaagacctt acttagattt ctcatattct
     2341 aacttggtag aagaattgaa agtaatttta gataaacaaa atatgaaaaa ggttgtactt
     2401 atagggcaat cggcaggggg atttgtggca cagtccttta ttgtaaaata tccaaatatg
     2461 gtagaaggac taatgatgat tggaacttgt ccatatggaa ctgactatta tagtaagtca
     2521 gatattttct ggttaaaaca gactgaatgg atgttaaagt tattcccaga taaatttttg
     2581 cgaaacataa tatctaagat gtgtggagct acgtctgaag gcagaaggag tatgatgaaa
     2641 atgttagaag gctacgaaaa aaaagaactg tgtcatctca tgtatctggg atttgctggg
     2701 ttcataccag aaattcaaga cttaaatata cattgtccag tttgtttggt ggttggagat
     2761 aaagacaaga cgggtaaagt gagaaaatat aatgagcagt ggcacaacaa gactgggtat
     2821 ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg tcgataaacc taatgaacta
     2881 aatcagatta tggatatatt tattaagcaa ttaaatatat aaaaattaca tactcattag
     2941 gtaaattatg tagtatatgt gtttatagat ggttatagaa ataaatatgt aataaactac
     3001 ttaacactta aaaagtggat ttgtccagag cataatacaa agcatgatat ataaattttt
     3061 aagcgaaata catcaaaaga agtattagaa ttagtggaga taaaacagct attagagatt
     3121 ataaataaat agttttaaag gtactacgaa tatagaggaa ggtaaatcat acgttattca
     3181 aggattataa atgattagtt taaaagtact atcggacagg tctctgcata tttaaacctt
     3241 agtaaaggta gttaaagtag tagtttaaaa actaaaaaat tatataaagg aattaggtat
     3301 aagaaaatga ttgagagtat accagcaaaa caaattttac aaaaagtgaa atttgacaat
     3361 acaagatggt ttggaattga ctataatatg aatctctatc gtggttgttc tcatggatgt
     3421 atatattgtg atagccgtag cacaatatac aacatagaga attttgacaa agttagatat
     3481 aaagaaaatg tgatagagat tttaagtaaa gaattgcgtt caaaaagaaa aaaaggggta
     3541 gtaggcattg gagctatgtc tgacacatat aatccttttg aaaagcaact ttgtattaca
     3601 aagcaggcac tagatttgat ttcggaaaat cattttggag tgtctattga tactaaaagt
     3661 agtttggtgg ttagagatat accgattttg cagaaaatta aaaaaaataa ttctgctatt
     3721 gtaaagttga ctataaccac tgcaaatgat gaattatcaa agaaaataga accttatgta
     3781 aatccaagtt ctgtacgatt tgaggcagtg aaagaactta atgattctgg aattttttgt
     3841 ggtatattat tgactcctat gcttccattt ttgactgata ctaaagatga aatacgagca
     3901 attgtagaaa aagcacacaa ggctaatgca aaatttattt attgtatgta tggtgtaaca
     3961 atgcgtagtg gtcagcgaga gtttttttat gagcatttaa gagatatatc accaaaattg
     4021 gtatttaaat atcagaaaac atatggttta aactatgtat gtactgtaca aaataaagat
     4081 tgttgtgaga agttgttaag agaagaatgt gcacgttatg gtattttgac ggatatgaaa
     4141 gatattataa aagcgtacaa gaggtcggag tcatatactc aaataaaatt tttttaagat
     4201 aggctttgga tgagatataa tagtggtgat tgatgtgttg atattatagt tgattgttag
     4261 agaggatatt atagatgaat attagtatta atattttagg taattgctag agctgatatt
     4321 atagatgaat attaatattg atattctagg taattgctag agttagtatt atagatgaat
     4381 attagtattg atattttagg taattgctag agctgatatt atagatgaat attaatattg
     4441 atattctagg taattgctag aactaatatt atagatgaat attagtattg atattctagg
     4501 taattgctag agctgatatt ataatttatt attaatatta atattctagg gaattatcac
     4561 tatattttaa aaagtaaaaa tatatatcaa aatgaactaa ataagtactg taagaagttt
     4621 gttaaattaa ggtttgaata aagtgagggt ggagaactta tgatagaaat aaaaactata
     4681 gaaataaata aaaaaagtca aattgacctt ttaatattag ctgacccatg tgaagaaatg
     4741 attgataaat atctcaataa aggaactatg tacgcattgt atgataataa agaacttact
     4801 tgtatagcag ttgtaaatga aatttccaaa gaaatatgtg aattaaaaaa cattgctaca
     4861 tatgagcatt ttcaaaatat gggttatgcc tcaaagatga tttatcatct tttagatgtt
     4921 tatagtaaaa aatattcatc aatggttgtt ggtacatctg aaagtggagt tcctttttat
     4981 gagaagtttg gatttgttta ttcacataaa attaaaaact tttttgtaga taattatcca
     5041 gaaccaatct ttgaaggaga attacaatgt gttgacatgt tgtatctatc atataacttt
     5101 tagataggat aatattttta ggttaatata attaattaat tcatataatt atcggaatta
     5161 taatttataa attatgtata ataagagtat aatttattta gaagctgtta tattgtatta
     5221 gattcaaata agatatataa taacttgttt attttgaatt ttggaggatt aattatggat
     5281 tcaaataata atactataaa atcaactgtt aaaaagggta tttcttttgg ttcttgttta
     5341 gcaatgatta tttcttatac tgcatggaaa tctattccat gggctatttt tcatggctta
     5401 atgagttgga tatatgtact ttattattgg gttaagtatg catagattaa attaataaat
     5461 aagtatgtaa tattgtaaag tttaagaaac aaaatgtaag ggagtgtctc gaagtgaact
     5521 tttagtttat aaggcactct tttttgtatt aaataaaata tattttcatt acttaaacaa
     5581 tgaaaaagat gagtatctct attttaatac atacccttat attcattcat ttatcaacaa
     5641 tagttttaaa aagaaagcta tctaaaatct attgtaatct aagtgcttct ctttaaagtc
     5701 ttaactttag taatgatata taagtttata aggttcttat ttcaataatg caaattttac
     5761 ttagaagttc agactttggt atgaagaaat gggtaataat aaaaaagagg tgttgatatg
     5821 aaagaaaaat tagaagaaaa atctaagatt attaaaacat caaaagagta taaacgaact
     5881 ataattgaag aaaaggacag agaatggacc caaaaattaa atgacatact tagtagaggt
     5941 gatttttcta atgaaaaatt agataaaatc aaggatttaa ttccaaaaga aatattgact
     6001 agtgaaaatg ttggagaagt tgtaactgaa gatgggtatg ctaagcctga gtatgatgag
     6061 gtatttaaat cattgtttac tttaaataat gattacgatt tacttgcaaa ttttataaat
     6121 gatatcctca aagatgctcc atatgctaat aggaatatca aacagttcac tcaaataaaa
     6181 agaataatta aggttgaaac cgacccaaca attaattaca ttggtgagaa aagacctaga
     6241 cttgatattt tggctgaaga tgaagaacgt aaccatataa atattgagat gcaaagagct
     6301 cttgaagagg attatttgga aagagctgaa tattatctta gtcgtgtaca tggtagaaag
     6361 ttagaagaag gtaaagaata taaagaaatt ggaaaaactg ttggaataca tatactaaat
     6421 catgtaaaat ataatcacat agaagattat gtaaattgcc tgagattaac aatggacggg
     6481 catccagata ttttttctag taaaactgca ttatatttta tagaacttcc aaaaattcgt
     6541 aaatccagtt gtatagcaaa cagagttcta atatggggta aacttattga taatccttct
     6601 catatagata ttagaatact atctaaaacg gattatgtta ttaaaagagc acttgataga
     6661 ttaaaagaat taggtagtaa tgaggactat ttattaaatt taaagagagg tgcgtatatt
     6721 atgaataaaa gtagaaattt taaagaagaa atttttcaag aaggtattga aaaaggtatc
     6781 gaaaaaggta tcgaaaaagg tattgcaaaa gggaaaagag aagagaaatt taatatcatc
     6841 ataaaattaa aaaataaagg atacaatttg agtgaaatat gcgatattat agatgatttg
     6901 aataaatcag aggttgaaaa agtctataat caaaactaat actatgattg agatggcact
     6961 agataaatac aaagcttcct cttttaaatt taaattaata taaaactttg ttatttaaat
     7021 atggtagata taaagagata tataaataat aggtacaaag aggtgtataa ataaaggaga
     7081 taattcaaaa taaaagtttg aaccatctcc ttcttataga aaaacaaagg ggttgattta
     7141 tatatgaaat tttatactat acagacttta gaattttgga ataacaataa aaacaataaa
     7201 tatctatcaa atgattattc ttttatatta gaagactttg atattccata caaatggatg
     7261 tatagtcaaa tgacaaatag aatatatgaa tttgatgata gtatgatatg ggtttggacg
     7321 aagagacctg atttaagatg tagtggttat ttagaaaaag gaaaaaaagg tgttttactt
     7381 gaaatagaaa taagtgaaga caatgtgtta ttatcagaat ttgatttatg gcatttacct
     7441 ttaatggata ttactgtaga gctttatgaa gatgaacaaa ttgaaaagaa ggtaagttgg
     7501 gaaagaatat ttgacttaga actgtgttca aaattaaatg aagttaatat agaagatttg
     7561 acattgcaag gagtgacatc taaagtgcct ctatctaatg taaaattaat aaaagaattt
     7621 gttgcaaaat aatattttta ttgaaattta ttcaaaaaag ttatatattt ataacaagag
     7681 gtgttataaa tacataactt tttaaggggg attttatatg tctaaacaac aaactggtat
     7741 taaatcaaca cttatttcag aaatattggg aatttcttat tgtattggag ctacattagt
     7801 aaataagtat ttttttaaag gaagtagtat tgaatatctt cctataatta tttgggtatt
     7861 agctatggga gctcagtggg tctataataa aaagtatgat gatttaattg atgaggcatc
     7921 aaaatgtata ctatcaagag ttaatgatat tgcaataaaa gtattgtttt tttctgtagc
     7981 tatagtatct atatttcttg taactttgga cataaatgtg tcaaatttaa atataggaat
     8041 gtctttatta gtcattttgt ttattcaatc cttgttgaag ttaattctat ttatatattt
     8101 tgacagaaag gggatataca attaggtgtc agtattacta aagacaaaaa ttaaatccta
     8161 tagagaaaag aaccttatga cacaaggtga acttgcaaag tcagtaggag taagacgtga
     8221 aactattgta catttagaaa atggtcgcta caatccttct ttaaaactgg caatggatat
     8281 tgctaaggta tttgatacaa ctgtagaaga gttgtttgaa tttattgaag aaaataatat
     8341 ttaaatttca tgtattactt atgcattaga gaatgaatta attataggta aaaaaccaag
     8401 cttatagatt agagaataaa tgacttatag atgaaaaacc aagctaatgg attaaagagt
     8461 gaatgattta tagattaaga agtaagctta tagattagag aataaatgac ttatagatga
     8521 aaaaccaagc taatggatta aagaatgaat gatttataga tgaaaaagaa agcttataga
     8581 ttagagaata aatgacttat agatgaaaaa ccaagctcat agattaaaga atgaatgatt
     8641 tatagatgaa aaagaaagct tatagattag agaatgaatt aattataggt aaaaaacaag
     8701 ctaatagatt aaaaaatgaa tgatttatag attaagaagt aagcttatag attagagaat
     8761 aaatgactta tagattaaga agtaagctta tagattagag aataaatgac ttataaataa
     8821 aaaccaagct tatagattag agaataaatg acttataaat aaaaaccaag cttatagatt
     8881 agagaatgaa ttaattatag gtaaaaacca agcttataga ttaaaaaatg aatgatttat
     8941 agatgaagaa ctaaacttat agattaaaaa ataaatgact tatttataga tgaaaaacca
     9001 aacttataga ttaaaaaata aatgatttat agatgaagaa ctaaacttat agattaaaaa
     9061 ataaatgact tatagatgaa aaatcaagct catagattaa agaataaata acttattaga
     9121 tgaaaaacca agcttataga taagaagtcc atgcttataa gccagaaaat gaatagttta
     9181 atagattaaa aaatacatat ttgtgaatta tagaatgaat aactccataa actagaaaat
     9241 ttataataat atctatatga aaattagaat tattttatca caaaaaaatt aagttaaata
     9301 aatatgaaat tacttccaaa tatacttcca aaaacatgta atcgcatata taatataatt
     9361 ataaaaataa taaatatttt tatagtagta gttaattatt aataaggagg ggtcaaaatg
     9421 cctagcatga ttatggaagt tgtaggagta ctatttatag ctattggatt aatcttgaca
     9481 ttcattgaaa taaaaggaaa tttcccttct ttttatggtt ttggtcaaaa ttaaaatgct
     9541 gtaggtatta tagcagcagg aattgcttta attttcttta gaaatggcat agatacatag
     9601 attctatagc tacttatggt gtatttttag caatagaact tgttgtaata gttgcatatt
     9661 tcttaataag aaaaaaattt actaaagaag ataaacattt aagaactaat taactaaatt
     9721 atctactata aaattggaaa attaaaaaca aataaaagtt tgtttttaat tatttaaaat
     9781 ccaaatttga ttatttttaa agaaactatg ttctgtataa aactagaatg tggtttcttt
     9841 tttatgtata aaatattttt tagcaaaagt taaaaggtgt tttataatat aaaatagtta
     9901 attgtagatg ttaataaaag aaaattatgt atattttaga taaagataag agagtatact
     9961 atattttaat tcttttttca ataaagctta ttgaaaagaa ctttaactta gaagagtatc
    10021 accaaattgc agatacaata tcataagcag ttatttattg acaacccttt ttaataaata
    10081 tatcatttga agtatactga cgaaaaggag ttgaataata atggacatct tattggttga
    10141 agatgataag aatattcgag aaatggtata cgattttctg attagtgaga attactctgt
    10201 taaaactgcc attgatggaa atacagctat tgaaatattt aaaaattatt catttgatat
    10261 tattttactg gacttgatgt taccaaagtc tagtggtttt gaagtattaa aatatatacg
    10321 aagtgtgagt gttgttcctg ttattattac tactgcgaaa gatagtgaca cagataaaat
    10381 gctagggctt aatctaggtg cagatgatta tattacaaag cctttttcaa tcgtagaatt
    10441 gcttgcaaga attaaagcta atattagacg tgcaacagtg tatagcagtt caagaaactc
    10501 tcaagatatg gttattcggt ataaggattt gattataaac atatcaaaat atgtagtttc
    10561 aaagaatgga attgacttaa acttaacaca tacagaattt gaaattgtta aattactagc
    10621 gactaatcaa ggacgtgctt tttcaaagga acaattatac aacgctattt ggaaagaacc
    10681 ttattatgga aatgaaaatg tgctaaatac tcatattaac aggttaagaa ataaattgtc
    10741 tgatggtata gaaaaaaata attatattaa aactctttgg ggaattggct acaaaatgga
    10801 ggaagaataa tggagttatt tttagttgta atttcgattg tactatttgc tctctactta
    10861 aaagaacgaa ataaatcaaa gaaaatggaa agtgattata agtatatcaa ttcaagactt
    10921 cgtgacataa caaatgatga tgaaattaat tatattttag taccatctga tatttcgatt
    10981 gtaaaagaaa cagcagtaga tataaataaa cttgttgaaa agttttacag caggcaaatt
    11041 aattataata gaagtgaaaa agcgatattg caaatattta caaacatatc acacgattta
    11101 cgaactccca tcacagtatt aaagggttat attgaaatgc tatatcttca aagtcaaaaa
    11161 gaagaattat cttcttcaat gagagcaatt gtggaaaaaa tgcaaacaaa ttcaaatgag
    11221 cttgttaatt caataaataa cctgtttaat atggcaaaaa tccaatctgg agatatgata
    11281 ttaaatcttc aaaaatccaa tctaacacaa ttatgtcatg agattatttt agagttttat
    11341 gatttgctag ataaagaaaa ctttaatgtt gaagttaata ttgaagatag acctatatat
    11401 gcaaatgttg acacagaagc attacaacgt atactcaaaa atttaattga taatgctata
    11461 aaatatggtt cagatggtag atttttaggg atttcactat atgaaaagga aaaacatgtt
    11521 tttatagata ttgaggataa aggttcaggt atttctgaaa ctgaaaaaga gcatattttc
    11581 acaagagctt atactgctga cagaaaaaaa ggcaatggtt taggtttggc tatttctcaa
    11641 gggcttgcaa actctatggg aggttcaatt tatgtaaata gtagacctaa tgtaaaaaca
    11701 gtttttacgg taaaattgaa aagttagaaa aaagttagat ttaaaagaga gaaaagacaa
    11761 ttttattttg tatactgttt ttagaaagga gggagtatga tgaactatat catagagacc
    11821 catcaattaa agaaaatata taaagataaa gctgttgtta atgctgttaa tattcatgtg
    11881 aaaaagggag aaatttatgg atttgttgga ccaaacgggg caggtaaaag tacagtaatg
    11941 aaaatgttgc taaatcttgt taatccaaat tctggagaaa tagttatgtt cggtaagaaa
    12001 gtggcagaaa ctgattttga aatattaaaa aagattggta ctattattga aaatccgtat
    12061 ttatatgaga atttgactgc taaacagaat cttgatttac actgtgaata tatgggttat
    12121 tataacaaag aacatatatc agaggtattg gaatgtgtcg gattgtcaaa gcaatcaaac
    12181 aagaaagttt caaaatattc tttagggatg aaacaacgtc ttgcaatagc tagagcaatt
    12241 cttactaaac cagaattatt gattcttgat gaacctataa atgcactcga cccagagggg
    12301 ataagagaaa tgcgagaatt attccgtaag ttaaatacag attatggaat aactatattt
    12361 atttctagtc atattctttc agaggtagaa caaattgcgg acactattgg aattattcaa
    12421 gatggtaaac ttattaaaga aatttctatg tcagatatac acaaatatca aacagattat
    12481 gttgaagtag atgtagataa tgtggagctt gctggatact tgttagaaaa agaatttgga
    12541 ataaaagact ttaaaattac ttcggaaagt tgtattgaaa tatatgattt acgaaaagat
    12601 gtaaaagaaa tatccaaaat atttattcaa aatcaaattg gtataaatag cattggacgt
    12661 aaacaaagtt ctttagaaga ttacttcttc caaactacag gtacaggagg taaagaaaca
    12721 ctatgataca tttaatgaaa ctagagctaa aaaaagttgg gctaaaaaga tattttttat
    12781 tttcaatgct ggggattttc ttatgtatgt tttttgtctt tactggattg aatgataaat
    12841 ctacttcaat atatgattat gatgttacat ttaggacaat cagtttattt ttctgttttt
    12901 actatattat attatttgct gtgttgaatg tggcatatat aataaatgaa tatacaaata
    12961 aaacaatctt gattttattc tcttatccac ttgaccgtaa aaaacttatt ttatcaaagt
    13021 tacttttgat tacgactctt atgatatcat caatgatagt tggatatatc tgttgtttta
    13081 cattcgttgt tgtgttggat aagtatttta atttagttat tggagatttt catatttcaa
    13141 ttttatctta ttggatacca ggagcaatta aaagtattat tgtattttgc tcattaggta
    13201 tatggacatt cgttgttgga atgataagaa aatcaatacc tgttaccctt gtcagttcaa
    13261 ttatttttat caatttaaga caatttattt tagctggtac aaatacaact caagacagtt
    13321 tgatttctgt ttttattata gttggtataa ctgtgattgg tatatattat atactaaatc
    13381 ataaagttac tgaaattgac tagatactgt ggataggagg gtagttatta attgtatctt
    13441 cttgggataa taatgatttg ctattatatc agctattatt aatattatta gagttttaat
    13501 aatattgcac aaccatttga agttgagcca aagcttgatg aattaattga atggtactat
    13561 agtcagagtg aagtatctat taaagttata gctgaatttc attatagatt tgaattgata
    13621 catccatttc aagatggtaa tggtcgcata ggtagatttg ttatgctaaa acagatgtta
    13681 gaaaataatc ttcctataaa aattgtatct tgggatagtg aggaattgta tagaaactcc
    13741 ttaaattctt gtagtttagg taattatgtc cctttaattg aatatttatc aagtttagaa
    13801 gattttagag aagtatataa aatgttatgg aaataattgt ttgcagcaaa aaaagttttt
    13861 atataaatat atttttatct atgctattta acgctaattt cttagttatt cttagcttgg
    13921 actataccca ctattttaac tatataaaat agtggatata gtttaactat ctctttataa
    13981 ctatttttat gtatcttagt tgtttcttaa tatggggaca gttcataaaa ctaaaagtga
    14041 tttttaattt tatcatttat aatttaccat tacatcagaa aaatgcttat cattagaatt
    14101 ttcataaaag gtacctattt ttttcaatta actgaatcaa ttctattagt cttaatcttt
    14161 catatttcaa attgataata taagaaaata aaaaataaaa aattttaaat tttttattta
    14221 ttgcgaaaaa aataaaaagg tgctatactt tttgtaacat attattcaaa ttattaagag
    14281 atagtaaaca tttatagttt aatttgtagt acactagcat gatttgcaga acaatgaatt
    14341 aatctatagt ataatagttt aatttaaagt acaacgattt aatctatagt acactagcat
    14401 gatttgaagt acaacgattt aatctatagt acactagcat gatttgaagt acaacgattt
    14461 aatctatagt ataatagttt aatttgaagt acaacgattt aatctatagt acaataactt
    14521 gatttgaagt ataacgattt aatctatagt acaataactt gatttgcaat acaatgaatt
    14581 aatctatggt ataatagttt aatttgaagt acaatgaatt aatctatagt ataatagttt
    14641 aatttgaagt acaacgattt aatctatagt ataatagttt aatttgaagt acaatggttt
    14701 aatctatagt ataatagttt aatttgaagt acaacgattt aatctatagt acaataactt
    14761 gatttgcaat acaatgaatt aatctatagt ataagagcat gatttacagt acaataattt
    14821 aaattgtagt acaaaagtac tatcaaataa gaatttctac tttaaaaata ttactaaaag
    14881 taattaaagt ccagtaattc aatataatat ggcaaaagaa tagaaataag gtgagagtag
    14941 tgaaagtaaa gtcaaattta gatgaaattt tatatcataa gattattgaa agtcttattc
    15001 gtggtgaata ctctgtaggt caaaaaatat tactaaatga cctttgtgaa aaatttgaag
    15061 taagcagaac accagttgta caagcagtaa aaatgctaaa taaagatgga gtgcttacta
    15121 ttatgacaaa tggtaaagtg tacgtaccag agtatgaata tgatatggta aaacaagttt
    15181 gtgaaacgag aacactgatt gaaacatatg ctttagaaaa aatgatgcaa gaagaggaag
    15241 aggtttttca acaaaagcta gatgtaataa aaagatactc tgataaatgc gaggagtact
    15301 atcgtcaaga aaaatctgta gaacttgcac tagcagattt ggaattacat aaagccattg
    15361 tagaaggggc aaataatgag attttaaatg atgtatatgt tggtatacaa ggaagattta
    15421 tagtagtgaa ctatttaatt cgaccattaa aaaatcgtaa ttatgaagga acagtacaag
    15481 accattttga aatattaaaa ttcattgaag acagagatac aaaaaaagca gtagataaac
    15541 taagaaatca tattcaagga actattaaaa gattctgtga agatgaataa taagttttga
    15601 agttactata tatacttatc tattaagttg ctaaatgtag tctaagtata atctaattat
    15661 agtttaaaca taatctaaat ataatctaat aaatataatc taaatatagt gtaaataagt
    15721 ctaagtatga attaaatata gattaagtat agattaagta tagattaagt atgaattaag
    15781 catgaattaa gcatggatta aatatacttt aaattacaaa tattacaagg aagccagaag
    15841 gcttcctaaa aaaataacct ttataaaatt ttttatttta aatttaaaat tttttatttt
    15901 aaatttaaaa taaaaaatga aagaggtggt agagagtttg aaaaaaatta ctaaagaaag
    15961 gatagaaaat actatgaaaa agctatttac gagtgaaaca tttttggaat atcaatttat
    16021 aagtgattgc caaatatcac cagatggagg atatacagca tttattgtaa aaaaagctga
    16081 tatcaaagag aatggttaca caagccaagt ttatatatta aacaataaaa ctggcgaatt
    16141 aaaacaaata acgtctataa actctgttgg agcatatgcc tgggaagatg aaaatactat
    16201 cttgttccct gctttgcgaa atgaaaaagt aaaagaagct gtcaaaaacg gtaagcaatg
    16261 catgagttac tatgctttat ctttaaatgg tggagaagca gaagaacttt ttagactccc
    16321 tatcaaaggt ggtaaattga atcctatagg aaaaggtttg tatgcagtta ttgattctta
    16381 tgacaatgac cgacctattg tagagggatt gccagaagaa gaacaacaga aaaaaataaa
    16441 tgcttataac aaaagacatt atgaacattt caaagaaatt ccttatgctg tcaatgggga
    16501 gggatatatt agcagaaaaa gaaaacgcct atatgtatac gacagtaata taaatgagtt
    16561 gaaggcaatt acagcaccta tgtttaatgt tgtaggtatg aaaataagtg atggaaaaat
    16621 cttatatata gggcaagagt ttaaagatgt aaaaggtttg aaaaatgggg tctatgtatt
    16681 tgatacaaac acaaacacaa atgtctgtat attagaaaaa gataagtata ttataaaagg
    16741 ttttgaatta tatcaaaatc aagtgatact aaacctaact gatgctttat cttatggaaa
    16801 tggagaaaat ggagattttt atactattga tattgatacg aaagaaatga aacttctttc
    16861 agggcatcaa catcattgta ttggaaatac agtaacatct gatgttaaaa tgggtgctgg
    16921 tcagacaaca aaggtagatg gagaatatat ttattacact tctacagtag atatggactg
    16981 cattattgaa agaatacata ttccaacagg aaaacaggaa aaagtaacac agacaggttc
    17041 agttgatttt atagatgtaa aagatggcaa tattgtatgt gtgggttgtt taggaaatgg
    17101 attaccagaa gtttatacag tagagaatgg aacattgtgt aagaaaacac atttaaatga
    17161 ccatatttta gaggaatata aaatttctgt gccagaatac atagagtcaa aaggaagttc
    17221 taaatgggaa atacaaggat acgtatataa accagtagat tatgaggtgg ggaaaaaata
    17281 tccagctgta ttggcgattc atggaggacc aagattgaca tatggaccat attttatgca
    17341 tgagatacag gtatttacaa gtgcaggtta ctttgtattc ttctgtaacc caagggggtc
    17401 tgaaggtcgt ggcaatgctt ttgcagatat taggaaacag tttggagata ttgattatat
    17461 agattttatg gaatttacag atactgtact tgaaaaatat acagacattg ataaaacaaa
    17521 attggctgtt gaaggtggaa gttatggtgg atttatgacg aattggataa ttggtcatac
    17581 aaaccgtttt gctgttgctt gtgctcagcg ttctattgca aactggagtg gcatggaggg
    17641 tacaacagac attggttatt atttctgcaa aggacaaaca ggtgcttctc atatggaaaa
    17701 ccacgagctt caatggaaac agtcacctct tgcttatgct gataaatgtg ttacaccgac
    17761 tttattttta catggtgaaa aagactatag atgctatatg caagaagcat tccagatgtt
    17821 ttcagcattg aaaattcatg ggtgtcctac aaaactttgt ctatttgaag gagaaaacca
    17881 tgaactgtct cgttctggta gaccaaaaca aaagctacaa agattagttg aaatgctaga
    17941 ttggttttca gtatatataa aaaaagataa cataggacag tagaaaggag aatataaaaa
    18001 tggaaaaaat aaagattgaa aattcagata tttttgaata tatattccct catgatattt
    18061 cttgttcacc tgatggaaag catattgctt atattatatc aaatatcaac gaggaaaaag
    18121 actgctatga acatgactta tatgtgatgg acatcaaaac agaaaaacaa atacatatga
    18181 ctcaaacaaa agatgtaaca tcatttagtt ggatttctaa tacagaactc ctttttacat
    18241 ctaaaagaaa taagccaaaa tcaggtacaa cagattttta cactatttct atagaaggag
    18301 gagaagcaaa aaaagcattt tctattccaa aagcttgtag cgtacctgta tcacttggaa
    18361 ataaattgtg gttactgaca acaaaaaatc caacggattc taaaaaatca gaaccagata
    18421 gggcagtaga gggtgtagat tattggacat ttacagataa accttttata agagatggag
    18481 aaaatttttc tcaaagaaga cgtgtaacat tagaattata tcaagaaggt gaaaatataa
    18541 caaaagccat tacaccaaaa ttttgtgaag tagcaggtgt agacgtttct tctgataaaa
    18601 acagaatttt atatactggt cagatttatg aagattgtgc aactcctttt tctggacttt
    18661 gggagtatca tatagacagt ggtgaaacca aagaacttgt accacaagga aagtaccaaa
    18721 taagtttggc aaaatatatt ggcaaagata aagtaatgtt gcaagcttca acattagacc
    18781 gttctatcac acagaatcat gatattttta tacttgattt gagtactaat gaaataaata
    18841 tgattgctag tccagatggt atgtatgcta cattgctgga tgtagatgca gtatatggtg
    18901 gtggacgtag taataaagtt ataggagaca aatttattgg tgcaagaatt tgcagaacta
    18961 tgacagaatt taatgagttt gatacaaaga caggtaatat acgtatcatc acaaaagtag
    19021 atgcttttac atcttttgat atttacgaca acaccatgta cacagtaatg ttgaaagatt
    19081 atgagttagc agaaatatat agcattgata tgactacagg tacaatgaaa aaaatgactg
    19141 ctttttctaa accatattta gatactcata aggtttcatt acctgaaaaa ttgacgtttg
    19201 tagcaaaaaa taaagaagaa gttgatggtt ttgttatacc acctatagat gctaaagaag
    19261 gcgaaaaata tcctgctgta ttgttcattc atggtgggcc taaatgggca tatggctata
    19321 tgtttacaca cttgaagcag tgtgtaacat caaagggaat gtatgtgatt tattgtaacc
    19381 ctcatggtgg tgacggttat ggtgaaaaat tcttagaaat ggtagaaaga tgggggtatg
    19441 ttgactatga gcatcttatg gaatttgtag atacttgtat tgaaaaatat cctgggattg
    19501 atgcagaccg cttaggggtt gcagggggaa gttacggtgg ttatatgaca aactggataa
    19561 ttggtcatac agaccgtttc aaagcagcag tttctcaaag aggtattagt aacttgatta
    19621 cagcttcttt aattattgac tttggggata gaattatgaa gcagacttgt ggagataaga
    19681 cgccttggaa tcatgaggag gttctttgga atcattcacc tattaaatat gtgaaaaatg
    19741 ttaagacacc aacattgttc ttacatagtg atagggatta tagatgtttt atgggggata
    19801 ctttccaaat gtttacagca ttaaaacagc ttggtgtaga tacagaaatg tatttgtttc
    19861 atggagatac acatggattg tctagaaatg gaagaccatc taatagaatt gcaagagcaa
    19921 atgctattgt agattggttt gaaagatatt tataataagg gggttattct atgagtgata
    19981 tgttattatg gtttagtgtc gtattagttt tatttgctat tggggattta attgcaagca
    20041 aaacaaaagc aaaagtatca gctgtatttg ttacactact tttatttttg attctatttg
    20101 tcacaaaagc aataccagct gatattattg aaaaagcagg tatgaccgca gcagctagtt
    20161 ggagtgttcc aatgattatg tttagtatgg gaacaatgtt gaatgttaaa caatttatag
    20221 acgaatggag aacagtattg acagcatggc ttggaattgt agcagttata gtctgtgtct
    20281 cattatgtat tcctttgttt ggaaaatcaa cagtattgac aagcatacca gttataaatg
    20341 gagctttacc tgctactact attatgacac aagcagcact agaaaaagga ctgacacttg
    20401 cagcagctac agcaacagtt gtatttgcaa ttcagaagtt tattggtaca ccaatagctt
    20461 caagagcagc gttacaagag gcaaaccgtc ttttagtaga atatcatgaa gcaaaatcaa
    20521 aaggcattga tttagcaaat gttgatactg ataagaaaga agcagagaat acaggcacaa
    20581 aagtaaaaca agctttctgt gaaaaatacg ataaatatta ttctacaaat gtttgtatat
    20641 tttttatagc attattctct tatctagggt atgaactttc agagattatt catgtaaatt
    20701 attctattgt atgtcttgtt gttggtgtta ttgtaacacg tattggtatt gtgccaaaag
    20761 acattttgga aaaaggaaaa atcaagggat ttatcaacat ggttgtgttt gcagctgtta
    20821 ttccttcttt agcaaaagtt tctttgacag atttaattag tctttttgta ccaattgtgg
    20881 gtatgtttgc agcttctatc attggtatat tccttatgat gaaggtgtta ccaggttgga
    20941 aaattatcgg ttctaaacct cttgcttttg gagtagggtt ttgtcagatg ttgggattcc
    21001 caactacata tttaatttca aatgaagtgt gtaatgctgt tggagaaacg gaagaagaaa
    21061 gagcatattt gatgtctaag attatgccta agctggtagt tggtggtatg gcttgtatga
    21121 tttctattgt ggtagctggt cttatggtac caatgttgta gataggtttc atagttaaaa
    21181 aagtggtatt aatttatagg agaagctaaa aagtagtatt aatttataaa ataattttat
    21241 attagttaat ttgttataag aatattttct ttttataatt ataattttga ttttataaga
    21301 tagcagattt tttatttgct atgctaatag ttataatttg tttttatttg ctaaaatgca
    21361 actttaattt gatttatgta taatataggg tatttaataa tatttttgaa gaatttttat
    21421 aaaaaattaa aaagaggaga aaaaagggag tgccacaaag tgaacttttt agttctaagg
    21481 cactcttttt tgtacgaaat cgaatatatt ttcaacgttt taaaagtgag aaagagactt
    21541 atctctagtt tgagatagcc aattttaatt attaaaatta ttttttgttt tatttatcaa
    21601 agagtattcg tgttttagaa gttagtattt tttattttat atatacaagg ttttccgttt
    21661 ctaatgtttt cttttttaaa ataattaggg tattcaatta tagtaaaagg tggatggtat
    21721 tttggagtga tattatttaa agcacagtat tgaatgttga aatcctcaca ttttagagta
    21781 ttatcttttg taatcaaaaa aatcttctat acttatagca aggttacttc tgcaaatatt
    21841 ccatacataa taatatccca atttaaattt ttttaatata gagtttacct ctagtctaca
    21901 ctcttgaata actctttcct ctatccaaat tcttataaat tcatcccatt tattactatt
    21961 tatttattat tatctaaatt ttttagtatg tttgtaatta gaaatggatt tattccttta
    22021 tgttttttat aaatagttta taacttaatt tatcattaaa caaatcattc atacaggatt
    22081 actttttctt ttatatattc aattcttact tttaataaat atttaataca tcctatctta
    22141 tgtagtttta aaaagtgttt atggctatta acaatctcta atatttcatt catatttatt
    22201 ttgtcatagt aatattttac atcttttata ttgacaaaaa agtgctctaa gtgtatagtg
    22261 tgtatatgat aacgtcacac tgaaagtgat aaaaaggggg tgataaaaaa tgaaagtgct
    22321 catttctaac acatcagata agccattata ccaacaaatt aaagagcaaa ttaaagaagc
    22381 aatatttact gaagaactga aagaagggga tgcaatgcca tccataagaa attttgctaa
    22441 tgatttaaaa gttagtattt tgactattag acgagtgtat gaagaacttg aaaatgaagg
    22501 ctttcttaca cgccaagttg gaataggaac atttatttca actggaaatt tagaaatact
    22561 aagagactca aagagacgta tagttgaaca aaaaatggca gatatgatta aagatgcaaa
    22621 agcactagga attacacaga tagagttaca agaaatgatg gatattctat atgaggagga
    22681 ttaatatgga aaatatttta gaagtttata acttaaataa gacttatacc gatttttcat
    22741 tatctaatat aagctttgca ttaccagaag attgtataac cggatttata ggtgttaatg
    22801 gagcaggaaa aacaacaaca atccgttcga tattggatgt cgtaaagcgt gatagtggaa
    22861 aaataaaaat ctttggtaaa gatttcgatg gaaatgagaa ggaattaaaa aatcgaattg
    22921 gagttgtctt tgatgattca tatttctatg aggagttgtc aatggcagaa atgaaaagta
    22981 ttattgcacc ttcatatgat aattggtgtg aagaagatta tcaacaaaat atgaagcgtt
    23041 ttaatcttaa cccaaaacaa aagattgata cactttcaaa aggaatgaaa atgaaatttg
    23101 ctttagcatt agcattatcg cataaagctg agtttttaat tatggatgag cctacaagtg
    23161 gtttagaccc actaatccgt agtcagatac ttgacatact aacaggatat atgaagaaat
    23221 gtggtcgtgg tgttttcttt tccactcata taacatctga tttagacaaa atagcagata
    23281 tgattgttat gattgacaat ggacgtatta tattagagcg tgaaaaagat actctattgg
    23341 acgaacatag aaaagtaaaa ggggatattc aatttctaaa tgatgaaacc aaaaaattat
    23401 ttttaaattt agaaattaca ggttatggtt ttatagggat tacctcaaaa attaatgaag
    23461 tgcaaaaaaa tatgccagat attttaatag agcgtcctac aattgaagat attatgcttg
    23521 catatattga aaggagagag taattatgtt gattcattta gttaaaaaag atattctgat
    23581 tgtaaaaaaa tatgtattgt tgatattatt tattgcaata ggaatcccat tatttatatt
    23641 atggcgttta cctccatttg cacaaatatt gggatttgta ctatcaatta tattttcaga
    23701 atttatgctg tgtcagtatc tattattaaa ggagagtcaa tattcaaaag catctgtatt
    23761 actttgttca actccttatc ctagacgtgg cttagttcaa tctaagtatg ttttatttgc
    23821 aatggtattt atctattgta cattagttta ctggattgaa aatttactta ttccacaaat
    23881 aggaactttt gacttgacat atattttgat tgttttatta ataagttcta ttatttatgg
    23941 agtttatatg cctattcaat acaaattagg atatgaaaca acaaaattct ttttcgtgat
    24001 gataatcatg gcttcatcat ttgtattacc attaactata gctcacagca attttgaaat
    24061 tccatttata tatcagtatc catccatgat agtgaatatt gtattgttcc ttttaagttt
    24121 aattatactg ttagcttcaa tgtttatttc tattaaaatt tttagtaata aggagcttgt
    24181 ataaggtaag ggggactttt atatgccaca tttaccagat tgggctgttt tactttgtat
    24241 cttacttcca atagtatata aagcttgtca gatatttaaa agtaaaaaat agagtatata
    24301 tttatattgc catttttatt gagtgaagtt agatggtatg aaaaggttac ttgaaaataa
    24361 agttacttgg tgtgggagag taacatcata atattataga ttatatgttt cttgtgaatc
    24421 aatttgtatt ttttaatagg gaatatccag taattttgtt ttccattgat tatttttcaa
    24481 ttatgagtat gattgtgtgt attgtatacc atcttttaag gtgtttttaa aaaattatat
    24541 cgtcaaaata taaatctaag gaatcctata aataaagctt tttaatttta gaaaactaat
    24601 aaaaagtagg acgttaataa aaagaagctg tctcaaaata gagataagct tctttttcat
    24661 tgctgaaatg acgaaaatat aattgattta atataaaaaa gagtgcttta tgaactattt
    24721 attttgaaca gtagatacac ttgcactcaa tttagttact cctaagcttt atgaactaaa
    24781 agtttatttt gaagcttatt tttattaaaa gagattttat ttttctaaat aatcatctat
    24841 atacagtatt gcgtttcttt tatttattca taatttattt tcttattaaa gtttttctat
    24901 attcatattt tctatattac tttactttta attttatacg aattatcaaa aataaaatag
    24961 ttattagaaa aaaataaaag taattgcaaa gcttgcaatt acttacttct atattaatta
    25021 ctataccata aaataacaaa aaaatcaagt cgagtaatcc ttcaacttaa atgatatact
    25081 aggtattaat aatttttaat taataaaata taactagagg aaaatctaca ttttctcaat
    25141 ttctttaatt aaattgacct ctaattaagt aattataagg agaaaaatta tgaatttagc
    25201 aaagggatta gatgttttaa aaatttcatc aaatgtattc ggtgaagata aagttatgta
    25261 tattcctgta atctacacag aagatgatgc aacacttatt gacacaggtc ttccgggtca
    25321 aggtgattta ataattgatg ctcttaataa aagtaacacg agctttgata agttaaaaaa
    25381 tataataatc actcatcatg atatagacca tataggaaac ataaattatc taagagaaaa
    25441 atctaagaat aatatcaaag tatatgcata caaaagtgaa atcagctata taacaggaga
    25501 agaaactcct tttaaacttt atatgttgga acaaatggta gataaaattg atgataagat
    25561 gcttagtatg ctaaatgtta tgggacttgg atttaaatca tcatatacta aagtagatgt
    25621 gtcattagat aaccatgaaa aattgaatct aggtgaggaa atagaagtta tacatacagg
    25681 aggtcacact agaggtcaca tatgtcttta tttaaaagag tctaaagtac ttattgctgg
    25741 agatttgtta caggtagaaa atggagaact taagcctgtt gatgtgatgt acagcaacaa
    25801 acaagagtta aaagatgcta ttaaaaatat aagtaattat gatatagaaa cgatagtttt
    25861 tagtcatggt ggattatatc aaagaaacat aattggaaca ttgaaaaacc taattattga
    25921 gtagtactat atgatttaca ataatttctt aaattactta aaattatatc taaagatgta
    25981 gaatcaaaat taaatataag taataagaat tttgctttaa aattatactt aagtgttaag
    26041 ggtgatatgt agtattacaa gtattttaga atataatttt ttatagtaat tttaaagttc
    26101 tacaatactt cagttgaaaa atatttaaca gcatagtata ataggaaaga aaatatttta
    26161 aataaacttt agtatacaaa gtgaagaaaa atatttaaat catgtatcat aatgcaaagt
    26221 aaagaaaatc atttaaatta tatatcataa aaataatatg aagtattgaa cactcataat
    26281 attagagact ttaatatttt aattgatagg ttatattatt tataacatta aaaatacaat
    26341 ttacataaaa tagacatatg aaaaataaaa aattatataa taattatggg aatgaggttc
    26401 tcccttggat gataatatcc taaactgcca atgagctgat gacttctatg atatcataga
    26461 tgccaccagc tttttttgtt gtttaaatcg ataaaatgga ggtgaagtac ttactaaatt
    26521 tttatttttt aaatttgaag aaatatttaa ttaatagaaa aaatagtaga ttatatgtta
    26581 attattttat taagaatggg ttaagagtgt acctatatcc tatcaattta aaaaacaatt
    26641 atgatagtca ttacattgac tacagttgat gtaataaaac tttcaaaaaa tcttatgatg
    26701 caaatagaaa ggaattaaaa tgatagaaaa atttaaaact ttaatatgga ttaaaatgat
    26761 aataagaatt aaaagcgttt ataaaacata tggtggacta ttttttttag gactcatagg
    26821 tataccagtt ggtgcaatta taggtcttat agataccata tttggaacgg ttcttctaaa
    26881 agttacagat attcgagaaa catatccaat gtatctgata ccatttttag cagtagtagg
    26941 agttgttatt gcttattgct attttaaatt tggaggaaaa agtagtaaag gaatgaatct
    27001 aatatttgag gtagggcatg gagaagaaga aattattcct ttaagattgg ttccatttat
    27061 aatatcaggt acatggttaa cccatttatt tggtggtagt gcaggacgag aaggagttgc
    27121 tgttcagatt ggtgcaactt tttcacattg ggtaggtaaa aggttgccta taaaaaatgc
    27181 ttctagtatt tttctagtaa ctggtatggc agctggtttt gcaggattat ttgagacacc
    27241 aatagcagca atactatttg ctatggaggt tttggttgct ggttcactgg agtatcaatc
    27301 gctttttcct gcgtttactg cttcttttac agcaagtgca gtatcaaaag ctttagggtt
    27361 agagaaattt tcttttgcac tttcttctaa agttgtattt gacttgtcaa tattttggaa
    27421 gcttattgtt ttaggaatta tttttggaat ggttggtgga gcatttgcat ggtgtctgaa
    27481 attgtcaaaa agaaaaatag ggaatagatt aaaaaatcct atgataagga ttgctattat
    27541 tggagtatgt cttagtgtat tattcctttt attttataaa ggaagatatt caggtttagg
    27601 gacaaattta attcaaaata gtttctatgg tggagaaatt tattcatttg actggctgtt
    27661 aaagtttata cttacaatac tgactttgtc agcaggtttt caaggaggag aggttacacc
    27721 tttgttttcc attggagcta gtcttggtgt attgttagca ggatttttta atttgccaat
    27781 tgaattagtt gcagcgcttg gctatgctag tgtctttggt agtgctacaa atactttttt
    27841 tgcacctgta tttatagggg cagaagtttt tgggtacagc tatttacctt atttctttgt
    27901 agtatgcgct atttcatata tatttaatat ggataaatct atttattcac tacaaaaaat
    27961 ttcaacaaaa caataaataa aacaacataa tgtatgatta gaagagaaag aatattagag
    28021 aaaggatagg gtaggaatat gttgacaagt ttagctttga tttttttatt aggaatggcc
    28081 tcgggaggta tttttaagag gataaaatta cctaacttat tgggaatgtt acttacggga
    28141 attatattag gtccatatgt tttaaatttg attgataatt caatactaga tatttcttct
    28201 gaccttagaa agattgcatt gattattata ttaacaaggg ctggattatc tttagatata
    28261 aacgatttga agaaagtggg aagacccgct gtacttatgt gctttattcc agctactttt
    28321 gaaattatcg gaatgatagt tttagcacca aagttactag gcgtttctat attggaagca
    28381 gcagtcatgg gtgcagtagt aggagcagtt tctcccgcaa ttattgtacc taaaatgctt
    28441 aaattaatgg aagaaggtta tggtacagaa aaaagtattc cacagatgct tttagcagga
    28501 acttctatag acgatatttt cgtaattgtg atgtttacag tattcacagg attggcacaa
    28561 ggaaatagta tatcagctat tagcttttta caaattccag tatcaattat attaggtgta
    28621 atagttggag cagtgattgg aatctattta gcagtatttt ttaagaaggt tcatatgaga
    28681 gattctgcta aaggggtgtt gcttttaagt atctcatttt taatgataag tttagagact
    28741 gctttagagg gtattgtgcc attttctgga ttattagcag ttatgagtat tgggattttc
    28801 cttcagataa aatatcgtgt agttgcaaga agattgtcta taaagtattc gaaactatgg
    28861 gttggtgctg aaattctctt gtttgttttg gtaggggcaa cagtagatat aagttatgca
    28921 tttaaagcag gaattggagc tgtaatttta atatttggag ttcttttatt ccgtatggta
    28981 ggagtattct tctgccttat taagacaaat ttgacaataa aggaaagatt gttctgtatg
    29041 attggctata ttccaaaggc tacagtacag gcggcaatag gaggtgtacc acttgcaatg
    29101 gggatggcat ctggacaact tattttgacg ttagcagttc tagctatact tataactgca
    29161 cctcttggag cttttggaat tgatgtaact tataaaaaat tattaacttc tgtaaaaggc
    29221 gagaataagt aaaaataaag attattttaa ctggtaagtt atattttgga aaaggattat
    29281 actattgaat acaatataaa aaaatcattt gggagattaa gaaggtagaa gttaagaaca
    29341 gattttaagg tatgttatga ttattagaat tgattttgtg aaattattat tgtaatattt
    29401 aattatatag ataaagaagc ggagtgataa ctatgtgttt aaaaaaatta tcaaaattag
    29461 agttagttat tatgaaattt atttggaatt tagatataaa aaccaattca tatgagatta
    29521 ttgattatat gaaagaagaa cataatttac cagaaaaagt agctttaaaa actttatcaa
    29581 aactatcaaa gaaaagattc ttatatgttc aagaaacagg caagtgtatg tattatacag
    29641 ttgcaatcaa agaaaaggca tatttagagt ttatatcaag aaatgtgcaa aatcttttga
    29701 agaataattt tataaggaat ttattagtat catttcacga agaggagcta acagaggaaa
    29761 agataaagtc gttagaaaac tgggtagtaa attgggaaga agcttatgta taaatgttat
    29821 tatttttatt acaatttaga taattttggt tttgtaagct ctgcaactat ttttagatgg
    29881 ttgcagagtt tatttttatt ttttattccc ttaagcaata cttaaaagta aacgcattta
    29941 tatttactaa cttcaattac ttctaatact taaattgctt tttaaaaata ttttgatatg
    30001 tcatctctat agtaatctag aacttgtttc tattctttat ataaagtttt ta
//
LOCUS       DAMJVI010000039        29319 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.041, whole genome shotgun sequence.
ACCESSION   DAMJVI010000039 DAMJVI010000000
VERSION     DAMJVI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 29319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.041"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            274..2916
                     /gene="adhE"
                     /locus_tag="PC545_001844"
                     /gene_synonym="adhC"
     CDS             274..2916
                     /gene="adhE"
                     /locus_tag="PC545_001844"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDF2456612.1"
                     /translation="MEKKEKVVEKSNVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNARIVGQPAHVIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTAASGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGSTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLERLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
     gene            complement(3276..3617)
                     /locus_tag="PC545_001845"
     CDS             complement(3276..3617)
                     /locus_tag="PC545_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456613.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(3644..4315)
                     /locus_tag="PC545_001846"
     CDS             complement(3644..4315)
                     /locus_tag="PC545_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456614.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVDGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIDDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKADRIISIIDGE
                     LKLTL"
     gene            complement(4327..6903)
                     /locus_tag="PC545_001847"
     CDS             complement(4327..6903)
                     /locus_tag="PC545_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2456615.1"
                     /translation="MKDNLKIVLRYIKSYKARSLAIVVSIILATSLVVGIGTLSRSAQ
                     QADVDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYASTDVNEKLPINIL
                     YANKNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETF
                     KVVGILEDRFIEKKKGQCEIFSNLNESELNDFTTYIEFNEGSDINTNIDNIIKNAMLD
                     EKSIGRNSMLIESTMKNGTLDNSSKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLR
                     AIGSTNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFV
                     IPDKIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTKVNYLISKLT
                     LNMSVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYM
                     NADFILSNVYLNEIGNNTSFKDIKGFNDNQIDKIKNIDGIDKVKTASILNTRIEVNKI
                     NRLDYYEAINSTPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINL
                     KKMKKENLAIVYVPEISKTQKYTKTFTPGEGTPAVDIKVGDTIKVKYPKGGMDTGLYE
                     SLKDNYEYLEYEFKVGAIISYPFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYA
                     DLNENANKKKINNLLGKIGSEVPGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFII
                     SVLNIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGII
                     IQFKMYYMQGFVSYGLGFSIDYKIYILVILANIIVGILATYIPSRKINKISIVEAINI
                     TE"
     gene            7111..8046
                     /locus_tag="PC545_001848"
     CDS             7111..8046
                     /locus_tag="PC545_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2456616.1"
                     /translation="MANNFVFPIVILTLFIFSNLIMNKYKFYKELDILKRGILTFFIS
                     CFALILFMEGTKYIQLRLGYSFLLEGVLNIILFLVLIILSFYTGFKVIRLINNNNEQE
                     YKLQKLDSKINHKDNIIIKLRSQKHDYLKHLQVVYSLLNTGLDEDAKEYIMNISNSFK
                     SKNSRYGKVSYLDAIASLKYEECLEKDISFDVYIQDFMDNLEIEPNDMSSVLLNIIDN
                     AIESLSTIKKEKKYIRVHAYEDEFQYIIAIKNNGPKIHNVESIFWNGFSTKEGQDRGY
                     GLHLVKNILDKYDYSILVNSDDFLTEFVILIPKYY"
     gene            8065..8802
                     /locus_tag="PC545_001849"
     CDS             8065..8802
                     /locus_tag="PC545_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456617.1"
                     /translation="MIKVLICDDDKIVRDDIANIAKESGYVDYVKKVKDGIEAIEFIK
                     NEKIDLLIIDIDMPYKNGIDSAEEILSIDKDIHIVFVTGFADYSLESFKVHPIDFVVK
                     PFKKEKILDSINIAIDHINSHKIANNNFIEDTLFVYKIRKQIHMINFDDIVMFEKNSR
                     AINLHTKDEDIIKFYENFDDLKKRLPPNFFMTHRQYIVNLRFIHKVIPINKSSLEIRF
                     INFQENAILNKSLEKDFLYRFYRVKRF"
     gene            complement(8988..14045)
                     /locus_tag="PC545_001850"
     CDS             complement(8988..14045)
                     /locus_tag="PC545_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDF2456618.1"
                     /translation="MLERTRVEKNPKIIENFKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISNYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQKVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMLSKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEIYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETNFDLSNTPIDKDFDARKEQFESLKHTLEILNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNRETVLSLLRKLELTDVELDKITNLLSLKTSSIRQTVIKILLEQDDSNLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTARQELYNKLVLLIEDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDDSNSSSKLNKIISNISLSTREDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLDLDYKLILDILFYSISTCGYNETEKSWF
                     SSIYSNVLDSKKQNKIYTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEIYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTEEQVNREMISGYNASNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPRSFIDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACTIALENLA
                     RSAGFGDVNRMMWALETEKLNEVAHLFKPKDIDGTSIWIDVDENGKASTKVQKGNKSL
                     KSIPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGETYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQIQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PILFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKINNVNFEGSHA
                     LIAGNLGEYSVHMGSGIVHKKGMGALNILPIHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            complement(14070..14489)
                     /locus_tag="PC545_001851"
     CDS             complement(14070..14489)
                     /locus_tag="PC545_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456619.1"
                     /translation="MQIYFRVKAVGKRKPMLELTPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIINFLTNEEIESQADIGKVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSSINLKSGDIFTFIRLTLLAGRMF"
     gene            complement(14723..14974)
                     /locus_tag="PC545_001852"
     CDS             complement(14723..14974)
                     /locus_tag="PC545_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456620.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            15063..15794
                     /locus_tag="PC545_001853"
     CDS             15063..15794
                     /locus_tag="PC545_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDF2456621.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFDKLNDVYKSLPVGNCSGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTRKNPCPFKDTNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMILDSNIYSSGNIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEYKIRKRTIDRIKRIVLV"
     gene            15853..16764
                     /locus_tag="PC545_001854"
     CDS             15853..16764
                     /locus_tag="PC545_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2456622.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYVFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVCIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKFIEAGKFELNNEMFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLYQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            16815..17627
                     /locus_tag="PC545_001855"
     CDS             16815..17627
                     /locus_tag="PC545_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDF2456623.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            17736..18428
                     /locus_tag="PC545_001856"
     CDS             17736..18428
                     /locus_tag="PC545_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2456624.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYEDMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            18515..19456
                     /locus_tag="PC545_001857"
     CDS             18515..19456
                     /locus_tag="PC545_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456625.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLNQDNKKNEEISNNKNL
                     NNKQDRNIQNKESNEEGSNDIKEKNNSDSNVSVNNKSEKQEDNTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNSETNNIN"
     gene            19493..20650
                     /locus_tag="PC545_001858"
     CDS             19493..20650
                     /locus_tag="PC545_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2456626.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVVIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDSILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASKNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKTKTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            20802..21362
                     /locus_tag="PC545_001859"
     CDS             20802..21362
                     /locus_tag="PC545_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2456627.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVSNWIDIEKIIKQGI"
     gene            21390..21881
                     /locus_tag="PC545_001860"
     CDS             21390..21881
                     /locus_tag="PC545_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456628.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMDDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            22110..22661
                     /locus_tag="PC545_001861"
     CDS             22110..22661
                     /locus_tag="PC545_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456629.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDIDHFIEDNGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYIVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYI"
     gene            22747..23679
                     /locus_tag="PC545_001862"
     CDS             22747..23679
                     /locus_tag="PC545_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HDF2456630.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKDVISHNYKYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENLLDEFTTLTEATHWSGTTYGEES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEPLKTLLCVGGVHFEK
                     SFSDIIKNKEYNISIGHVLPNQWIVSGMYDDESGFEKLEKCINSIEGGIDCIVFHDKL
                     KGTYKEQCRKLGEKLNVPVFKHKILKNPKDLPIW"
     gene            23734..24597
                     /locus_tag="PC545_001863"
     CDS             23734..24597
                     /locus_tag="PC545_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2456631.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDRVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFLKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKIICEIFSNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            24844..26706
                     /locus_tag="PC545_001864"
     CDS             24844..26706
                     /locus_tag="PC545_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2456632.1"
                     /translation="MNYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVNGAQFQVVIGNSVVDVYDEILKVCPLDTNDSTVKTEEKINLGTRFIE
                     FIINIFQPLIFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLVAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPNIAYNAQVFPAILCVS
                     FLATMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAFLPFMVATGMHKALIPYSINTIGKLGYEALYMTSSLAHNISESGAC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAMLGVVFSSAISGTFLG
                     LFAVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNTV
                     ESSIGDVVEEELEEEKEVNILVTPVEGKVIDLSKVNDELFASKTLGDGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNNNGADYAYKVLNQSYGNVKKGSILFDVKGGI"
     gene            26709..28175
                     /locus_tag="PC545_001865"
     CDS             26709..28175
                     /locus_tag="PC545_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDF2456633.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGGKGWSVSDVAKAHFDADV
                     KDYKSNNEITTKDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDKEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEEVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLATQRKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEHDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDITPGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRINYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIESNSIDFK"
     gene            28199..29035
                     /locus_tag="PC545_001866"
     CDS             28199..29035
                     /locus_tag="PC545_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2456634.1"
                     /translation="MNYIIKKVLNSSVVLVHDTKDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDIPVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSGDDLLFNQMKRSNPKEMLIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
BASE COUNT        10851 a         3511 c         4683 g        10274 t
ORIGIN      
        1 ttttatttag tcgaagtaaa ttattcaaat gaaatatatc tcatttagat aaaaaatttt
       61 agtaaaagta aaaaaaataa tatatttgtt aaaaaataaa ttgacaaaat attcagaaaa
      121 aaacttcttg acctaagaga gtaaaataaa taaaatctaa ggtatagaga tgaaatctat
      181 aaaattattt taagtataag ttaaaaatat agatgatgca tttaagatta ataaaaatta
      241 gagatgcata attattttag ggaggttttt agtatggaga aaaaagaaaa ggtagtagaa
      301 aaatcaaatg ttgaagtatg tagtcctgaa attgtcaaca gtgttgaaac attaagaatg
      361 agattagaag aaattagatt ggcacaaaaa gaatttgcaa catttacgca agaacaggta
      421 gataagattt tcttagctgc ttcaactgca gctaatcaac aaagaatacc tctagcaaaa
      481 atggcagtgg aagagactgg tatgggtatt gttgaagata aggttataaa aaaccatttt
      541 gcctcagaat atatctataa tgcttataaa gatactaaaa cttgtggtgt catagaaaaa
      601 gatgaagcat ttggttttac aagaattgct gagccagtag gtgttttatc agcagttata
      661 ccaacaacta atcctacatc aactgcaata ttcaaatccc taatagcact aaaaactaga
      721 aatggtataa ttttctctcc tcatccaaga gctaaaaact gtactataga agcagctaga
      781 gttgtacatg atgcagcagt taaggcagga gcaccaaaag gacttatagg ttgggtagat
      841 gttccttcga ttgaattaac taatgttgtc atggcagagg cagaccttat attagctact
      901 ggtggacctg gaatggttaa atctgcatac tcttcaggta aaccagctgt cggagttggg
      961 cctggaaatg tacctgcaat aatagatgag agtgcagata taaaaatggc tgtaagttca
     1021 atcttagtat caaaatcatt cgataatggt atgatatgtg cttccgagca agctgtaata
     1081 gtaccagaaa aaatatatga agaagttaaa aaagaattta aatatcgtgg agcacatttc
     1141 ctaaataaag aagaaactga aaaagtagga aaagtagtaa taattgatgg ttcattaaat
     1201 gccagaatag ttggacaacc agcacatgta atagccaaaa tggcggatgt agaagttcca
     1261 aaaacagcta gaataataat tggtgaagta gaatctgttg aattaaatga accttttgca
     1321 catgaaaaac tttctcctgt acttgcaatg tacaagtcta aaagttttga agatgcagtt
     1381 gctaaagcag aaaaattagt tgcagatgga ggctatggtc atacatcttc attatatgca
     1441 gactctataa atcatccaga gagagtagaa aagtttgtaa atgctatgaa gacttgtaga
     1501 gtattagtta atactccatc atcacaaggt ggtataggtg atttatataa ctttaaattg
     1561 gcaccgtctc ttactcttgg ttgtggttct tggggtggaa actcagtatc tgaaaacgtt
     1621 ggagtaaaac atttgcttaa tatcaagaca gtagctgaaa ggagagagaa tatgctttgg
     1681 ttcagagcac cagaaaaggt ttatttcaaa aaaggatgtt taggtgtagc agcaagagaa
     1741 tttaaggatg ttatggataa aaagaaagca tttatagtaa cagattcttt cctatataat
     1801 aatggatata ctaagaaatt aactgattta ttagatgaga tgggaattaa gcatactaca
     1861 ttctttgatg tagctccaga cccaacatta gcttgtgcaa gagaaggagc taaagctatg
     1921 gctgatttcc aaccagattt aataatagct gtaggtggag gaagtgcaat ggatgctggt
     1981 aagataatgt gggtaatgta tgagcatcca gaagtagatt tccaagactt agctatgaga
     2041 tttatggata taagaaaaag agtatatgta tttcctaaga tgggtgaaaa agcgtatttt
     2101 gctgctatcc caacttcagc aggaactggt tcagaagtaa ctccatttgc agttataact
     2161 gaccaagatt caggtgtaaa gtacccatta gcagattatg aattaatgcc aaacatggct
     2221 ataattgatg ctgatatgat gatggagatg ccaccaagat taacagcagc atcaggtgtt
     2281 gatgctttaa cacatgcatt agaagcatat gtatctatgt taagaactga accagctgac
     2341 ggtctagcac tacaagcagg taaaataata tttgaatatt tacctcgtgc atataaaaat
     2401 ggaaagaatg ataaagaagc aagagaaaaa atggcaatgg catctactat ggcaggaatg
     2461 tcttttgcaa atgccttctt aggaatatgt cactctttag cacataaatt aggtgcattc
     2521 caccatgttc aacacggtgt tgcaaatgca ttattaataa atgaagtaat caagttcaat
     2581 tgtgcagaag ctccaaataa aatgggtgca ttctcacaat acagatatcc tgattgcata
     2641 caaagatatg ctgaatttgc ttcttttgca ggtattaaag gaagtacaga ccaagaaaaa
     2701 gtagataatc ttataaaagc aatagatgaa ctaaaggcaa aagtaggtct tcctaagact
     2761 ataaaagagg ctggagtaga agaatctaaa ttcttagaaa gattggatgc tatggttgaa
     2821 caagcttttg atgaccaatg tacaggtgct aatcctagat acccattgat gagtgaatta
     2881 aaagagatat atctaaaagt ttattatggt aaataatatc tattgtgcaa cataggttct
     2941 attgggtttg tatataaatt attagatttg aatataaata gagttttatt agagacacgt
     3001 agaagtaact ataaataata gattataact taaattctaa atggaaatga taaaaagtca
     3061 gtaatttaat ggtaatttcg aatatgtgta tttaatcaat taactttaaa attagtcgga
     3121 aaattttttt attacatata attttaaagc ataatatata aaaaacttta tgaaaaaatt
     3181 ataaaaatat tcagaaaatt ctattgacta atttatttat catgatataa taagtttaga
     3241 aaagaaaaaa attggggcct attaaggccc ccttactatt ttttgataga tttatttaaa
     3301 attactttct cattttgatt ttttatattt attatagagt cagtataatc cacttcaaag
     3361 ttggcaaaag cagagaataa atctatatct cttaacaaat gaattgtcat acattgattg
     3421 ttcttattta gccttagaat caatgctctt acaaatggaa attgtattct agaattaaat
     3481 aagttagaac catcagtatc tatatctaag ctacgataat taatatctat aaatatatct
     3541 acatcatcat tttcttctct tattattctg tagtcaaaat ccaagtttaa atttaaatta
     3601 cctaaaatca ataacattta aatcctcttt tctataaata gttttagagt gttaatttaa
     3661 gctctccatc tattatagat ataattctat ctgcttttct agcaatattt tcattatgtg
     3721 ttattattat aagggtttgg ttatattttt ttatagaact ttttagtaaa tccatgactt
     3781 cttctgtagt tttactgtcc aaatttcctg ttggttcatc tgctaggatt atacttggtt
     3841 ttgcagataa agcacgtgca atagcaactc tttgctgttg accaccagat aattggttgg
     3901 gaagatgatt tttcctttca ttaagaccca ataaatttat aaggtcatca atatattctt
     3961 tatctatttt tttattgtct aactttagtg gtaattctat attttccttt acatttaata
     4021 caggaattaa gttatactgt tgaaagacaa atcctacctt ttgtcttcta tatcttgcta
     4081 attctttatc tgaaagacaa catatatctt taccatctac ctttatagta ccattacttg
     4141 gtctttcaag accacctact aaatgtagaa atgtactttt cccacttcca cttggtccaa
     4201 ttatagcaac aaattcgcct ttatttattt ttaaatttgc attctttagt gcatgtacag
     4261 atgtttcctt tttcccataa atcttaccta aattaatagt ttccaatata ggcataatta
     4321 tccctcctat tctgtaatgt ttatagcttc aactatacta attttattta tctttcttga
     4381 tggaatatat gtagctagaa taccaacaat tatatttgct aaaataacta ggatatatat
     4441 tttgtaatct attgaaaatc ccaatccata tgatacaaag ccctgcatat aatacatttt
     4501 aaattgtatt ataattcctg acacgatagt aattatactt gagagtacac catataatat
     4561 tccttcataa agtatcatat ttttaaagcc tctctcagag ataccaatag ctcttaacat
     4621 tccaaattca ctagttcttg aagttaagtt ataacttatg ttgtttatta tgttaagtac
     4681 actgattatg aacattattg ctactatacc ataactataa atcatagttc tagatgtcat
     4741 cttatcagaa ttttcttttt ctaccatcat atctactgtt gtagttccag gaacttcact
     4801 tccaatttta cctaaaaggt tatttatttt tttcttgttt gcattttcat taaggtctgc
     4861 ataaactaag ttatacttat ctacaccagt taattttttt aggtattcgc tactcgttat
     4921 tacatctaca ccatcatctc cagagtacat accattatct gcaaaaggat aacttataat
     4981 agctccaact ttaaattcat attctaagta ttcataatta tcttttagac tttcataaag
     5041 ccctgtatcc atcccaccct ttggatattt gacttttata gtatcaccta cttttatatc
     5101 tacagctggt gttccttcac caggagtaaa agtctttgtg tatttctgtg ttttagaaat
     5161 ttctggtaca tatacaatag ctaaattttc ttttttcatt tttttcaagt ttatatttcc
     5221 acttactaag tatttttcta atgactttat catctcatca ttataacctt ttagcttttg
     5281 ctttagtgta tatttgccag tagtcttatt ttttatgaac agaggattta gcttataata
     5341 aggagttgaa tttatagctt cgtagtaatc taatctattt attttattta cctctattct
     5401 tgtgtttaag atactagcag ttttgacttt gtcaattcca tctatatttt ttattttatc
     5461 tatctgatta tcattaaacc cttttatatc tttaaaagaa gtattgttac caatttcatt
     5521 gagataaaca tttgatagaa taaaatctgc attcatatat gtttgttctt gagtagattt
     5581 gttttgcaca tctaaaaaag aatatttata attgtctttt atgacgatta tacctcctat
     5641 actcatagat aatacaatta taatgaaccc ctttttattt ctaaagatgt ttttcgcaga
     5701 tatagatttt gttactgaca tatttaaagt taattttgat attaaataat taactttggt
     5761 tacattttta tcagctccaa aagtttttct gatagcatca ataggagaaa ttcttcttat
     5821 ttttaagtat gtaaagaaac ttataattag tattattagt attgtacatg caattgataa
     5881 gagaattatt ttatctggaa taacaaaagg tgtaacattt atatttcctt caaattgaat
     5941 atttcctaca gacctattaa atatttgtgc acctcccata cctatacata tacctatagg
     6001 cattgctatt agagcaagta tgaaaagttc ataaagcata agtttgaaaa tcctaaagtt
     6061 ggtggaacca atagctctta agacgccata ttcctgtact ctttggtata ttgatataac
     6121 atagatacta tatattacaa taccagcaaa tagactcata gcaattgcaa cgtatttact
     6181 tgaattatct aaagtaccgt ttttcatagt agattctata agcattgagt ttcttcctat
     6241 acttttctca tctaacatag cattttttat tatattgtct atgtttgtat ttatatcact
     6301 gccttcatta aattcaatat aagttgtaaa atcattcagt tctgattcat tgagattaga
     6361 aaaaatttca cattgtccct tttttttctc tataaatcta tcttcaagga ttccaacaac
     6421 tttaaaagtt tctggatttt ctttttgata taatttaaaa gtaaggtttt gatttatctc
     6481 tggttttaag cccatactat ttaaaatcca tttctcgact accacctcat tgctagcttt
     6541 tggaaatcga ccttttataa gtttagaatg actagtcaaa taatttttat ttgcatatag
     6601 tatgtttata ggtaattttt cattaacatc tgtagacgca tagtaagatg ttatacccag
     6661 atttttaata tctttgctag attttatgga ctttaattga tttttcttta tatttttaaa
     6721 gtatacatgg tcagttccaa gctctctttt caatctatct acatctgctt gctgagcact
     6781 tcttgagagg gtaccaatac caacaacgag tgatgtagca agaattatac tcacaacaat
     6841 tgctaaagac ctagctttat aactttttat ataccttagt actattttca aattatcttt
     6901 catatttacc acttcctttc ttaactaaat caattatata tttatctaaa atatatagca
     6961 aagtaagtat tttcaagttt taagtagtta attgtacaaa ttcatctgta tttttacaag
     7021 tttaattaat ctatatgtaa attttaactt tttacataat ttttttaaaa tgatataatc
     7081 cctatataaa ttgtagaggg gagggaaatc atggctaaca attttgtatt tcctatagta
     7141 atactaactt tgtttatatt tagcaatctt ataatgaata agtataaatt ttacaaagaa
     7201 ttagacatat taaaaagagg catattaaca ttttttatat cgtgttttgc attgatttta
     7261 tttatggagg gcactaaata tatacaatta aggttaggat atagtttttt acttgaagga
     7321 gtattaaata ttatactttt tttggtttta attattttaa gtttttatac aggctttaaa
     7381 gttataagat tgataaataa taataatgaa caggaatata aactacaaaa actagatagt
     7441 aaaataaatc ataaggataa tataattatc aaattaagaa gtcaaaaaca tgactatttg
     7501 aaacatttgc aagtggtgta ttctctatta aatactgggc tagatgaaga tgccaaagaa
     7561 tatataatga atatatcaaa tagttttaaa tcaaaaaatt caaggtatgg aaaagtatct
     7621 tatttagatg ctatagcttc attaaaatac gaggaatgtc ttgaaaagga tatatctttt
     7681 gatgtctaca ttcaagattt tatggataat ttagagattg aacctaatga tatgagttca
     7741 gtactattaa acataataga taatgcaata gagtctttgt ctactataaa aaaagaaaag
     7801 aagtatataa gagtacatgc atatgaagat gaatttcagt atattatagc tataaaaaat
     7861 aatggtccta aaatacataa tgttgaaagt atattttgga atggatttag tactaaagaa
     7921 ggacaagata ggggatatgg gctacattta gttaaaaata tattagataa gtatgattat
     7981 agtatacttg taaatagtga tgatttttta actgaatttg taatcttaat cccaaaatat
     8041 tattagttag gaggagaaaa gtctatgata aaagttttga tttgtgatga tgataagatt
     8101 gtaagagatg atatagcaaa tatagctaaa gagtctggat atgtagatta tgttaaaaaa
     8161 gttaaggatg gtatagaggc aattgaattt ataaaaaatg agaaaataga tttattgatt
     8221 attgacattg atatgccata taaaaatggg attgattctg ctgaagaaat tctttctata
     8281 gataaggata tacatatagt atttgtgacg ggtttcgctg attatagtct tgaaagcttt
     8341 aaagtgcatc ctatagattt tgtagttaaa ccatttaaaa aagaaaaaat acttgattca
     8401 attaacatag caatagacca tattaactcc cataagattg ctaataataa ttttatagaa
     8461 gacacccttt ttgtatataa aataaggaag cagatacata tgattaattt tgatgatata
     8521 gttatgtttg agaaaaattc aagagcaatt aatctacaca ctaaagatga agatataata
     8581 aagttttatg agaactttga tgatttaaaa aaacgcctac ctcctaattt ctttatgacc
     8641 catagacaat acatagtaaa tctaagattt atacataagg ttatacctat aaataaatct
     8701 tcattagaga taaggtttat aaattttcaa gaaaatgcca tattaaacaa atctctagaa
     8761 aaagattttt tatatagatt ttatagggtt aaaagattct agtgaagata gaagaaaaat
     8821 actttttgtg tgatataata agtaccataa agataatgta taagagaagt ttatataaaa
     8881 attatttgta attatgattt aatctatata aattacagta tagtatatat tggactttct
     8941 aatttttttt aagaaagtcc aatatatttt agggatagtt tttatactta taaattactt
     9001 aagatactag ggtctttaat cttactatct tcagcaagta atagtatctt agacataatc
     9061 tcagcagttt tagggtcttc atcagcgaat ggaaggaata tcttacctct actttgagaa
     9121 tgtataggaa gtatattcaa agcacccatc ccttttttat gtacaatacc agaacccata
     9181 tgcacagaat actcacctaa attacctgct ataagagcat gagagccttc aaaattgaca
     9241 ttgttaattt ttaataataa taaaagttct ttagcaattg ctatacgcat ctctacagta
     9301 gaatggctag tttcagggtc aacaccacca gcatgagcga cacttacaac aaggtctata
     9361 tcacgcataa cttctgagaa tagaattgga ggtatagaag ctaattctaa acttttataa
     9421 gtttttctat caaaaaattc tactgtttca atagtaggag gctctatatc tgctggtgaa
     9481 aaccaatctg caagtgcaaa catctttgcc actatatttt ctttataaaa tactttctgt
     9541 aatccctctt cataatcgac agtccaaagc tgatttctaa gaagtgctac agttttcttt
     9601 ggctgaattt ggtgaccagc atatctgcga gatatagttt tttcagataa ttcatcactg
     9661 tttattctat aatactcacg gaatacttgc ttaaatggtt gaacaatttg cttactaaat
     9721 aaatcatgtt ggaactcact ccacttacca gattcaaaca aatgaactgg atgtgcaagt
     9781 tttaatttat tttttgccgt caacttataa gtttcacctt ctatattgca aataaagaac
     9841 ttagacttat cattataaac atatccaata ttatcatcac atacccatac tagattttta
     9901 atcaaagggc agataactgg atttttatag atattggcaa tttctttaac tgtaaatact
     9961 atttcatttt tcatagcttg ttctaaactc tgttttgcac gagaatactg actctttaaa
    10021 tctttttgga cagatttaag ttcaagtaca tatggatgtt tagcaatagc ttttggtata
    10081 ctctttaaag acttgttgcc tttttgaact ttagtagatg cttttccgtt ttcatcaaca
    10141 tctatccaaa ttgaagtacc atcaatatct tttggtttaa atagatgagc aacttcattt
    10201 agtttttcag tctcaagagc ccacatcata cgatttacat ctccaaatcc agcacttcta
    10261 gctagatttt ctagcgctat agtacaagct tttgattcac tttcacgacg ttgtgcacca
    10321 aattgcttgc tttctttagc aaacttttga ataaattcat aacggaaagt tgcttctttt
    10381 aggtctcctt ttttcatagg aagtaaagag aaacttctca atttatcttg atttctattt
    10441 gtttcaattt ctttcatcaa catgtcttta tcaagcttac caagtgtagc atcagcatag
    10501 agttgtgcac gtctatgaga gttaccacca ctagttatat atttagctga cttgtaaagt
    10561 atattgaatc tttcttcccc aatagtttta tatgaatcta aaaaccaatc tacatcaaat
    10621 gcaccatcaa tgaaactacg tggtgaaata ggggagaata gagcaacctc tgattctttt
    10681 tgtgcagaga agccttcatt tatatgtgca tggaagtacc aaattgtact tttcagaccc
    10741 ttccagttta aatatttttc aattatgttt acccattgag gggcatacat agcagattct
    10801 atcaatctta actcagtaat gtcggtattt ttaagtaaac tttttaattt ttttgagtca
    10861 tcttctttag taggatagca atttttaagt agagaactta aaacatcttt ttttgaataa
    10921 ttgctaccat atgaatatcc tctctggaac gtttctttac caagagcgac caaaatattt
    10981 acaaaataat ttataccttc aaagtattgt atattattag ctagatgaga cacctctgtt
    11041 ggcatatcac ctctacgtga ttcaatttct ataattcggt ttgtaacagt ttttaaatta
    11101 tctttaagcc aagggtactt ttctaatgtg tcgccagccc atctaggatt tgtcaatgtt
    11161 cttattatat ttgatgcgtt gtatccactt atcatttctc tattaacttg ctcttcagtc
    11221 attaatccta attcatgtgc acgagcaaac acatttatgc tagtatagaa aggaaactta
    11281 tattctagca actgataatt tgaaaacgta gtgtagaaaa acttagtaaa agaaatatca
    11341 tcataaataa acgaatgtgc tttatcaagc caatagttta aaataggggc atttaaagca
    11401 gggcagtatt cattttgtct gtaatagttt ttagtaaatt caactctttg ttttttacat
    11461 tgttctatag tcaataagtt tctaagttta tagtatattt ctaaacaata gttaaatatt
    11521 tctttatggt caaactcatt tagtaaaact cttaaaagtt ttcttgtaag tccattaaat
    11581 ttaagttttt caagttttgt atatatttta ttttgttttt tagagtcaag gacatttgaa
    11641 tatatagatg aaaaccaact tttttctgtt tcattatatc cacatgtact tatactataa
    11701 aataaaatat caagtattaa tttgtaatct aagtcaagct ttttactagt ttctctccaa
    11761 agctctgcta atggataatt atcaagagta tcattagaaa ctttatattt aataggagtc
    11821 aagttataag tagaataacc taaaagtact ttgtctacat tacctgacca tctttcaact
    11881 tcatattcat aatctgcatt ttcagtaata agctcatcaa atttttcata aacttttatt
    11941 attttattta tatccgtatc gagaattttt tttgcttctt tttttgttgc tacagaaaca
    12001 gaatcctcac gagtagataa acttatattt gagataattt tattaagctt tgatgagcta
    12061 ttagaatcat ctttaaaatc aaattgttct ggaatttgaa ctttgttttc agggttatat
    12121 attccaaaac cattttcttt gcagtaatca ttttttgctt tacctaacaa attatcaatt
    12181 agtatttgaa cttgagatgt attcttttta gatttatcaa tatcttctat aagtaaaaca
    12241 agcttattat atagttcttg tctagcagta ttgtctttta ataagtttac aatttcaaga
    12301 cctccaaggc atttttgaga atttgaagat tttatcagtg aagaagcaat attttccaaa
    12361 tttgaatcat cttgttcaag taaaattttt attactgttt gtctaataga gcttgtcttt
    12421 aaagacaata gattggttat cttatctaat tctacatctg ttagctcaag ctttctaagt
    12481 agactcaata ccgtttctct attggaaaca ctcttatcac ttaaacattc caataaaaat
    12541 tcacgttgtc tatctgtctt tggttcattt acaaaattga ctataagtga agctcttaaa
    12601 tctgaactca taagatgaca attttccata agcacatcta taaattgagc attcatatcg
    12661 tatgctgtta aagtcatcat acatttaagt atttcatgag aagaaatttc aatccaagtc
    12721 catggaaata cacttcctga aaatgatttt gtctttgaat ttaaaatttc taaagtatgt
    12781 tttaaacttt caaattgttc ttttctagca tcaaagtctt tgtcaatagg agtgtttgac
    12841 aagtcaaaat tagtttctct gttttgccaa ctatagtagt agccaacaga accttctaca
    12901 tagaaatttg taattaccca tgctagtatc tctaaatcat cacccttaat gtttttagat
    12961 gctatagaat gtttaaaata tgtactttct gtatgcgata aaaagtaaag agctacaagt
    13021 tttttatatt tttcctgaca agaaagtaag ttttcaataa gagagtaaga gtcatatatg
    13081 ttgtaagttg cagtagccca taaggcaaaa taaatttcaa gattatcatt gctattaatt
    13141 gattctatac ggaacttttc atcagtcaat gatttatatg aaagttctaa acatttacgt
    13201 attattccag gtttttggtc tgaaaaacca agtcctgtcc atgtatccaa ggctcttata
    13261 acagaactaa atcgctctaa attgttgtcc aaaatagttt ttaacatgta tattgatgca
    13321 gaaattgtac cctcatctaa actctctaca atttgctgtc taagtccttc ttgttgtttg
    13381 gctgccaaaa gaagtttaga aagcatttca tagcatttat cactatgact cttcatgata
    13441 ccaattatca tttgtcttgt aagtagagca gtttgatttt ctccaaatat tacttcttct
    13501 aaagcactta ttactttttg atttcctttg tctatttcta gtgctatttt atcactcatt
    13561 atagagtcat agtacaagac cttatttgga agagtaagat aatctataac tgaaaaatca
    13621 taataaatta aatcaacata gtttgaaata aaccttataa tacctctaaa ataatccaca
    13681 agtttatttg agcgataccc tttacgataa tatcctcctg aatatgtata atccatcttt
    13741 cttttaatta attggcaaag ttcttctact tgttcagaag taaatattaa ttctaatgca
    13801 ttttttaaag gtccagaaag gagctcgtcc atatttttaa tttcgtagta gtggcaattg
    13861 tagtatttct ttatcatatt taagaatttg tcttcaggtt ctctataata gtatgaacta
    13921 ttttctatgc agtcccaaag agactttgct aagtcgtagt actgttttga agaagattta
    13981 gtaaaattat ttttaaaatt ttcaattatt ttaggatttt tttctactct agtacgttct
    14041 aacatttatg tacctccaaa ttatttattt taaaacattc ttccagcaag caaggtaagt
    14101 cttataaaag taaatatatc tccacttttc agatttatag aggaatcgag tttttcaata
    14161 acatcttcat ttataagcac tttgtagatt ccatcttcaa atgcctgtaa cgctacctct
    14221 attgcctttt ctacagattg tttattctca ttgtaaatag agccaaatcc aacttttcca
    14281 atatcagctt gagattcaat ttcttcatta gttaagaagt ttattattga tttttctggg
    14341 attttctcat tatattcaag aacattttta gatacaattt cagagatagc ttcttttagg
    14401 caattgatgt tgtcagatac atcaaaaggg gtaagttcta gcataggttt gcgtttgcca
    14461 acagccttaa ctctaaaata aatttgcata aaatcacctc gcaaaatatt aattatgtat
    14521 ttagaaattt aaaaatttat ctatttgtta tattatatca ttttgtgaat tattaatgtg
    14581 aattatggaa ttattttaca aaaaatagaa aaaaagctat cttaaagtct aaaaaagcaa
    14641 taaaagataa cttttaacta taattatata gaattataac tgtacattta ttaaatttta
    14701 attttagaat tatttttgat aactatcttt ttttatttat aaattttgct cctaaatatt
    14761 ttttgatatc ttcttcagga gaaaagtcat caataagtat ttctttccca gtgtcttcaa
    14821 atactacaag aacttcacag tcaccttgta agaaaaattt tctaactgta tcttctttaa
    14881 cttctctaac ttttattaca ttcttttttt ctttttcaaa gtcttttgtg taataacttc
    14941 ccatcatatt aattattttt gtatatctat tcataaaata cctccatatg cgattaaatt
    15001 taaaaaatat gttataataa ttgtttgtat taacttaata attataacaa ggagtaataa
    15061 taatgactag tataaaaaat aaagatattt taaattgtat agattattcc attaaaaata
    15121 agctctttga caaactaaat gatgtatata agtccttgcc agtaggaaat tgttctggat
    15181 gtggaaattg ttgtatggag tcagtaggta taaatttaat tgagtttttg aatatatttt
    15241 gttatcttga agatagagtt gatttaaaaa aaagctgtat aagtaaaata ttagattatt
    15301 attttgagga gtatacaaga aagaacccat gtccatttaa agatactaat aatagatgtt
    15361 taatatatga agttagacca ttaaattgta ggatgtttgg acattggaaa aaagaggatt
    15421 ataataaaaa tttagataat gttattgaga aaaataataa ctatagagag ttaatgaaaa
    15481 agcaatatgg atttgaaata aatgatgaag tagtaaatta caggatagat tattgtgata
    15541 gatttattcc aaataaagat tatttaagta aatctaagag attaagcttt tttgatgagc
    15601 ttatgatatt agactcaaat atttattcaa gtggaaatat agatatagac tttagggata
    15661 gaggtatagt tgaatatttt atagagtcat tactttatag aaatttagca tataatgtga
    15721 aaataaggat atctaaagaa tacaaaataa gaaaaagaac tatagataga ataaaaagga
    15781 ttgttcttgt atagttttat attagtaagt aataaattgt atcataaata ttgtcaaaat
    15841 gaggtgaaga aggtgaatct tacaaaagta aaaggaaata ccttttttat aaaaggtggc
    15901 acgaatacag gaatatatgt atttgaagat aacacagcac ttatgataga cccagggtta
    15961 tgtggttcaa gacctaggag agtaaataaa gtattagatg aaaataatat acaggttaga
    16021 tatattataa atactcatgg tcatgatgac cactatggag cttgtaatca atttaaagaa
    16081 tattataaag atgtgtgtat aatgtcatct gaatacgata aattatatat agaaaatcca
    16141 gaattgtttt caaagtatat tgttggggga aagagcaatg tatttatgga taatatctta
    16201 aaaaataaaa tgttggatga tataaaaata gataaattta tagaagctgg aaaatttgaa
    16261 ttaaacaatg aaatgtttga tataattgaa tttcagggtc atacacctgg tagtatagct
    16321 gttttgacga aggataatgt gatttttgtt ggcgatttat tgataggaga tgaaatttta
    16381 tcaaaatacg atttcttgtt tctatatgat atacaagaac aaataaattc attagaaaaa
    16441 ttaaaaaata tagattttga gtacttagtt ttagggcatg gcaagcaagt tatatcgaaa
    16501 gaagattctt ataaattaat agaaaaacat ttgaatgctt taaaaaaata tttatatcaa
    16561 ataagagagt tgcttgtaga accgaaatct ttagaaattt tattaaaaaa cattataaat
    16621 aacaataatt taagcaataa ttataaagag tatcattttt ttaaatcttc gttagtatct
    16681 gtcataagtt acttagttga tttagaagaa atagggtata tattggaaaa tggagaattg
    16741 ctttactata caaaaacaaa ataaattatg ataaaataaa gaaggtcata aaaaacaaat
    16801 aaagggtgag caatatggaa tacgaaaaat ggaatgagat actagcacct tatgaacatg
    16861 cagtagagga attaaaaata aaatttaaaa atataagaaa agaatattta gcaaaaggtg
    16921 aatattctcc catagagttt gtcacaggta gaacaaaaaa aatatcttcc ataatatcta
    16981 agttaaaaag gataaatgca aaagatatag aaactgaaat tgatgatatt gctggtataa
    17041 gaataatgtg ccagtttgtt gaggatatat atgctatagt tgatttgata aaagtaagaa
    17101 atgatatgac tataatagga gaaaaagatt atattacaaa ttacaaagat agtggttata
    17161 gaagctatca tgttataata aaatacccta taaactccat tgcgggttca aaggaaatta
    17221 tatgtgaaat acaaataaga actcttgcaa tgaatttctg ggctacaata gaacattcat
    17281 taaaatataa atatgaccat tacatacctg agactcttgc tgaaaggctt agaagagcat
    17341 ctgatgctgc ctttctttta gaccaagaaa tgagtgaaat aagagaggac ataatgaaag
    17401 ctcaggctat gtaccaaatg aagtctatag ctattagaga tactctaaat agaatacaag
    17461 agttatatga cttgggtgat acacataaag ctatgcagta ccaaagaaaa cttgatagaa
    17521 ctgaaacaga taaaaatata actgaaattt tggaattaaa acaagaaata gatatattat
    17581 tagagcaata taaggatact aaggtagatg ataaagtagt tcagtaaaaa ctacttgtag
    17641 aaaatttgct aaatattaat ggggctgcct cttaatggaa aataatttct aaaaagagac
    17701 agcctattta gtgtaattaa gaaaggaatg atattatgag tttgtatgct ataggtgatt
    17761 tacatttttc aacatcagtc aataagccaa tgaatatctt tggaagtaat tgggatggtc
    17821 atgaaaagaa aattatagat aattggaaag aagttgttaa ggaagaagat atggtcttag
    17881 tactaggaga tacatcttgg ggaattaatt tatccgaagc taaaaaagat ttagatataa
    17941 taagcaaact tccaggtcag aaaatactta taaagggaaa tcatgattac tggtggacaa
    18001 ccgttacaag tttaaataag ctatatgaag acatgcgttt tattcagaca aatttttatg
    18061 aatataaaga ttatgcaatt tgtggaggta ggggctggat atgtcctaat gatgttaaat
    18121 ttgatgagac tgatgaaaaa gtttataaaa gagaagaaca tagacttaga ctatcattag
    18181 aatctgctcg taaaagtggt cactcaaaaa taattgtgat tactcattat cctcctacga
    18241 atgataaatt ggaagaatct ttatttacaa agttatttga agaatataat gttgaaaagg
    18301 tgatttatgg tcacttgcat ggtaaagaat cttttaaaat gggattgaag gggattcgaa
    18361 atggagtaga atatacatta gcatcttgtg actatacaga atttaattta ataaaagttc
    18421 acgattaaaa aacataaatt ggtaaatact ataagataca tgattaaaaa taaatatttg
    18481 agggatttcg gttttgagaa aggagacacg acttatgagg tggatattag tttttatatt
    18541 tttatatctt attcctttag tagtgatatt cagaaattac aagaatttta agaggtcttg
    18601 tatctacagt agtatttatg tggtattagc tacaacaata atgataagta atatttatat
    18661 gagtggactt aataaaataa aagagtctat gtattatcac aattatattt ctgatgagac
    18721 atatgatgaa aagtatgtat caaattttaa ggaaaaagaa agtactgatt taaaccagga
    18781 taataaaaag aatgaagaaa tatcaaataa taaaaattta aacaacaaac aagatagaaa
    18841 tatacaaaat aaagaaagta atgaagaagg aagtaatgat ataaaagaaa agaataattc
    18901 tgatagtaat gtttcagtga ataataaatc agaaaaacaa gaagataata caaaagaaaa
    18961 taataaatat acaagtaaag aaaaaaatag tgaagatata aaaaaaatag atatggaaag
    19021 tataggaatt tttaagaaag aaatttataa tatagaaaga gcagctttag ttccaatgag
    19081 agaatgcata ccttacacta aggatatagc aaaaaatata actaaattat ctagtataaa
    19141 aaaagatgtt acaaatgcca gaaacaagtg taaagaagtt gtaaaagaat atgaaaatat
    19201 gcagattcca gaactttcta aggagaagta tacagaaaca ttgagtaatg ctagagatga
    19261 cgtaaagaaa gcatatgagc ttagagaaaa atctatggaa agtgctttga aattaataaa
    19321 tactaaaagc cctaaatata tagggaagat aacagaatat cttgatttat cagataagca
    19381 gatagaaagt tttaaaaata gattgaacga tttaaaagaa gaaataaata attcagaaac
    19441 aaataatatt aattaataag atttaaggat ataagttaaa agggagatta tgatgaataa
    19501 acagagtata ggaaaactaa atatgttttt tatggggatt actcaaagaa accttgataa
    19561 tagggatttt tttattgagt tagaggtgat ttttaaatct ggaagaaaag agtttaaagg
    19621 aattgcaata gaggaagata ataaatataa atttaatttt aaaggaaaat cagatttata
    19681 tacgtttaat gagttgctta agaatatttc taaagaggca gaaaattatg atggattagt
    19741 atttaaatat gttgagcgag gaactattgt tgtaattgaa ggagacaata agaaggtaaa
    19801 tgtaaaatat ttagataata aggaagaagt acctaaaata gatgaattta cggcatctca
    19861 aataaaaaac agagattatt atgtaaaagt aggacaagca aatgcacttt taaaagaaat
    19921 tggagtactt acaaaagatg gtaagataaa aaatgataaa ataagaaaat acaatcagat
    19981 agaccatttt gttgaattga tagatagtat tttaaaagaa ataaaggata aagactgtat
    20041 aactatcttg gattgtgctt gtggaaaatc ctacttaagc tttgtcttaa atttttatat
    20101 aaaagaagta ttgaagaaaa actgttattt tataggaata gattattcag atgtagttat
    20161 agaagcatca aaaaatatgg ctaaaaactt gggttataaa aatatgtcat ttataaaaga
    20221 ggatttgact aattatacac ctaataggga tgttgacctt gtaataagtc tacatgcttg
    20281 tgatacggct acagatatgg caataggtct aggaattaga gcaaaatctg aagctatagt
    20341 agtagtacca tgttgtcata aggagctatt agggcaatat agatatgaag ctatggaacc
    20401 aatattaaaa catggtgtat ttaaagctag atttgcagat ttaataactg atggtttaag
    20461 aacattgtta ttggagggca atggatatga tacttctgta gtagaataca tatctccact
    20521 tgatacacca aaaaatttaa tgataagagc gataaaaact aaaacgaata atgacaaagc
    20581 attaaaagaa tataaagaac tgaaatcaca atttggagtg gaaccaacct tagaaaaact
    20641 tatatattag ttaaaaatgt aaaatttacg aaaagcttgc ttaaatatgc aagctttttg
    20701 gtattatata ggtatagtca tatccagagt tttgatttat aattaaagct gtgctataat
    20761 atttgattaa tcaaatcaat aaatgttaca tggaggtaaa aatgaacaaa ttgaatatat
    20821 gtattgatat agatggaaca ataacaagcc cttatcattt tcttccatac ctaaatgata
    20881 tctttaataa aaatataact gaagcagagt gtataacaca taattgggaa gaattgtatg
    20941 gttcgggaat aagagatgtg tatgcggagt ttaataataa gtatatacac tcatatgatg
    21001 aagcaaagat tgtagagggt gctacagagg taattagcac attatctaaa ggacataatt
    21061 tatcttttgt aacggctaga catgagtgtt taactgatgt aactaaaaat tggctatcga
    21121 gacaaggatt ttcagatata gatgtttact tattaggaag tgattataaa gttgaaaagg
    21181 caagagaact gagttgtgac atatttatag aagataatcc tttaaactca gtgcaactag
    21241 ctgatgatgg tgttaaagta atacttctgg atactaacta taataaagat gttaaacatc
    21301 aaaatatagt tagagttagt aattggatag atattgaaaa gattataaaa caaggtattt
    21361 aaagatttgt agattgaaag ggggagcata tgatagattt aaaaattgaa aatagaaaag
    21421 gaaaaatcca tgtaaagagc agtgaaataa aagacatttt agaattacgt ccagacttcg
    21481 aatatgtaca agatatatca aatactataa atcaagaaaa tatattggta tttgattgcc
    21541 agttgacagg aaattctttt gatatggatg atttagatat agaagaaata ttggaagagt
    21601 taggtgaaga aatagatgag tcttatttca gtgttttatt tgaagatgta agagcgtatt
    21661 taaaagatgc tactgatgag atagaagcag acttacagga taattactta tttgataatt
    21721 tgagatgtta ttttgatata tacaatataa atcaagaatt tacagatttt aagtttgtat
    21781 ttttagtttc ttttaaagat ataaaaattt cttcattagc aaacctagct aagattgttt
    21841 ctaagagaca gcttataggt gcatctaagt tttatagtta aaatgaaaga ttattataat
    21901 aaaagctagt aaccttgtaa aaatataggt tactagtttt ttatttattc attttcacaa
    21961 aatttcagaa aactatttac attctaaaaa aagtaatgta tattaaacat aatgtacatt
    22021 ataatggata aaaagtttct attaaattac taagtttatt taataatcaa aataatttat
    22081 cttatattag tatgtattag ggggtattta tgcaaagttt aaatgaaata attgctgaaa
    22141 atttaaaaaa gataaggaaa gaaaaacatc ttagtttaga caagattgca caacttagtg
    22201 gtgtaagtaa gagtatgcta tctcaaattg aaaagtgtga ggtaaatcct actatatcta
    22261 ctttgaagaa aataactaat gggctaaaaa tctcatttac atcattgatg gagaggcagg
    22321 aaagtgacat agaattgatt caaaaaagtg atatagacca ttttattgaa gataatggaa
    22381 agtatataag ttatccaata tttccatttg actccaaaag aagatttgag atatttatga
    22441 ttgaaataga agaaggtgga aatttagatt caaatgctga acttccagga acacaagagt
    22501 ttataactgt ttttagtggc gaagtaacca ttaaaataaa tggagaagat tacattgtat
    22561 ctagtggaaa ttctataaga tttaaagctg atgtatcaca tatatataga aactcaggca
    22621 aaggtatagc taaaatgagt atggtagtat attatatata aataatttaa tggaattaga
    22681 gtgatattta gatgatttta ggtatgaaga tatattatat ttaagttata ggagtgtgat
    22741 ttatggatga ataataaaaa agcagttttt ttcttttgca gtgatttaaa gaaagaccca
    22801 gtagccagta atgtgataaa atgttgtgaa gaaataatgg atttaaatca aactgatatt
    22861 gttattgatg ataattttgt attagagttt aaggataaaa acaataactt atttcatttt
    22921 gtaaaaacaa aagatgtaat aagtcataat tataaatatt atcttccaat tttaaataga
    22981 cattttagag attatgattt tgcaggagtt gtaaattggc atgagggaga aaatgctcca
    23041 gaacatatcc taacagctca tacaattgga gatgttccaa caggagaatt tggaaattca
    23101 aatccaagat actttaaaaa tctaatcaat gctattgagg atataaaaaa tgaaaatcta
    23161 cttgatgaat ttactacatt aacagaagca actcactggt ctggaacaac atatggtgaa
    23221 gaatctaaat taattacaga atattctgta cctatgttgg atattgagat aggaagtagt
    23281 agtgatagtt ttaataatag tatagcaata caggtattgg caaaatcatt aattagagta
    23341 tttgattgtg atgaaccttt gaagactttg ttatgtgttg gaggggtaca ttttgagaaa
    23401 tctttctcag atattataaa aaataaagaa tataacatat caattggaca tgtattgcca
    23461 aatcaatgga ttgtaagtgg aatgtatgat gatgaatctg gatttgagaa actagaaaag
    23521 tgtataaata gtattgaagg aggaattgat tgtatagttt ttcatgataa attaaaaggt
    23581 acctataaag agcaatgtcg taaactaggt gaaaaactta atgtacctgt atttaaacat
    23641 aaaatattaa aaaatccaaa agacttacct atatggtagt tttatacagt taatataaca
    23701 ataagtcgga ttaattttta agcgaggtca aaaatgatat tatcttattt tgcattatta
    23761 attagagtag tgctattagc ttttgaaaga gtagttgtaa gattattagg tgatgaagaa
    23821 ggagatattt ataaaaatat agcttcatca tttctatttt tctttatagg gggagtatgt
    23881 ttacttccat ttagcataat ggatagagtt agtgattggt cgtttttaat accatgttat
    23941 ataagtagtt tagtctattc aattggttca gtagcgtatg taacgtcttt agcaactggg
    24001 gaagtttcac ttgttactcc aataaatagt ttaaattcat tgtttttact gcttttatca
    24061 gttatatttt taggtgaagg tttaagttta gcaaaagttg caggaatcat aattatgatt
    24121 ggtggagtgt ttatattaaa aaaagtaagc tcttttttaa agaatgtctc tgtaattttt
    24181 aacaatttac catgtagatt aatgttttta tacatatttt tgcagtcagt aggaagagta
    24241 ttggataagg ctttttttgt acaagtatct cctatattat attcaactat actgtatttt
    24301 ttcgtaggtt taaatttatt tatcttttta gcatttaaaa agaaacaaaa aataatttgt
    24361 gagattttta gtaataaaaa ggggctttca attttaagtg gtgctattaa tggatattct
    24421 tatttagcat tattaattgc tttaaataat ctagaactta gtatagcaga gccattttct
    24481 caagtatcta tgataataac tttaatactt gctcatttta tattcaaaga aaatataaaa
    24541 gaaaaaatac caggttcaat tttgatacta attggtgggt ggctactgtt actttaattg
    24601 atataaaaaa tgaattataa aggtgctatt agttagtatc agaaaattta aaaaagtatt
    24661 gacaattctt ttaggcaaat gttaatataa taatacaaaa tagtataaat agaagacatg
    24721 ttactggtaa tgcaggcagg atcgaatata ttgctttgat ttttcaaggt gtttatatag
    24781 gttctgcctt ttttgcatct tgaatggcca ttcataaaaa attatggaga aggagattac
    24841 aagatgaatt ataaaaaaac tgccattgac attattgaac aagttggtgg ttcaaaaaat
    24901 attttagctt tagaacactg ttcaactaga ctacgcttta agttgattga tagaagtaaa
    24961 gtcaatcagg atgctttaaa aaaagtacaa ggtgttatga gtatagttaa tggggcacaa
    25021 tttcaagttg tcattggaaa tagtgttgtt gatgtgtatg atgaaatttt aaaagtttgc
    25081 cctttagata caaatgatag tactgtaaaa actgaagaaa aaataaactt aggaacaaga
    25141 tttatagaat ttattattaa tatttttcaa ccactgattt ttgctattgc aggagctggt
    25201 attttaaaat cactgttgat gttattagca atgtttaata ttttaaaatc agattctact
    25261 atatatacat tattagttgc tatttcagat gcaacattct atttcctacc tcttatggta
    25321 gctgtaacta ctgccaatgt attgaaatgt aatcgtttag tagctattgc cgctgtagga
    25381 tacttattgc ttccagcaac aacaactgct ttaagtgaag gtgtcgaact atttggattt
    25441 acaattccaa acattgccta taatgcacag gttttcccgg ctatcttatg tgtgtctttc
    25501 ttggcaacaa tggagaaaat ttttaacaag tattcaccaa aaccaattcg aactttcttt
    25561 gttcctatga tgtctttggc aattactgtt ccaattactc taactatttt aggtccttta
    25621 ggatataacg ttggtacaat ttttacaacc attattttat tcttatataa caaaatggga
    25681 ttcttagttg taggattact tgctgctttt cttccattta tggttgcaac aggaatgcac
    25741 aaagcactaa taccttattc aattaataca attggaaagt tgggttatga agctttatat
    25801 atgacatctt cattagccca caatatttct gaaagtggtg cttgttttgc agtagccttg
    25861 cgaacaaaag atgaaacatt aaaacaaaca gcattatctg caggaatttc tgccttgatg
    25921 ggaattacag agccagcttt atatggtatt actttacaac ataaaagagc aatgttaggt
    25981 gttgtattct caagtgcaat ttctggtaca tttttaggat tatttgcagt aaaaggattt
    26041 gtaattgttg gaccagggct tgccagtatg agtatgttta ttgacccaaa caatggaaac
    26101 aatttgattt ttgcaagtat tggttttgtt attgcaatat taggctcgtt tattattaca
    26161 ctgattattt ggaaggaaga tacaaataca gtagagtcat caattggaga tgttgttgaa
    26221 gaagagctag aagaagaaaa agaagtaaat atcttagtaa ctccagtaga aggaaaagta
    26281 atagatttaa gtaaggttaa tgatgaacta tttgcaagta agactttggg agatggagtt
    26341 gcaattatac cgaccaatgg aaatttatac gcaccttgtg attcagaagt tgtgatgtta
    26401 tttgaaacaa aacatgcaat tggtttaaga acaaaaaatg gggctgagat tttgattcat
    26461 attggaataa atactgtaag tatgaatgga gatggattta aaacatttgt taagacaggc
    26521 gataatgtaa aagaaggaga tttattaatt cagtttgatt tagacaaaat ctcacatgct
    26581 aatttagatt caactgttat gattgtaaac aataatggag cagattatgc ttataaagtg
    26641 ttaaaccagt cttatggcaa tgtaaaaaaa ggaagtatct tgtttgatgt aaagggagga
    26701 atataaatat gagaaagttt ccagaaggat ttctttgggg aggagctaca gctgctaatc
    26761 aatttgaagg tggttggaat ttaggtggta aagggtggtc tgtatcagat gtagcaaagg
    26821 cacactttga tgcagatgtg aaagattata aatctaataa tgaaatcaca acaaaagata
    26881 tagaagaagg attagcacat cctgaagatg aagtaaatta tccaaaacgt catggtagtg
    26941 atttttatca tcactataaa gaagatattg ctctaatggc agaaatggga tttaagacat
    27001 atcgaatgag tattgcttgg tctcgtattt ttccaaatgg agatgataaa gagccaaatg
    27061 aagaaggttt acagttttat gatgatgtat ttgatgagtt gataagttat ggaattgaac
    27121 cacttgttac tatgtcacat tatgaaccac cattaaatat cgttttaaac tatgatggat
    27181 ggtattcacg acaagttatc aatatgtttg ttaggtatgt agaaactatc tgtgaaagat
    27241 ataaaaacaa agtgaaatat tggcttacat ttaatgaagt tgattctatg attcgtcatc
    27301 cgtacactac tggtggttta gttcgagacc gttttaagga taaaaacttt gaggaagtta
    27361 tttttcaagc tatgcatcat caatttgtag ctagtgccct tgcaacaaaa atttgtcatg
    27421 aaattattcc aaattcaaaa gtagggtgta tgttaacaaa attaacgtat tatccataca
    27481 catgtagacc agaagatgtt ttggcaactc aacgaaaaat gagaagtatt tatgcatata
    27541 gtgatacgca ggtttttggt gagtatccag tatatttact gtcgtatttt aaaaataata
    27601 acattcaaat agtaaaagaa gaacatgatg atgaaattat gaaaaagtat ccagtagatt
    27661 ttatttcatt ctcttactat atgtcttctt gtgaggcagc tgatacaact ggattagata
    27721 ttacacctgg gaatacgtta ttagctgtaa aaaatcctta tttagaaatg agtgaatggg
    27781 gttggcaaat tgattcaata ggtctacgta tatctttgat tgaattatat gatagataca
    27841 gaaagccttt atttatagtt gaaaatggat tgggagccaa agatatttta actgaagata
    27901 aaaaagttca tgaccaatat agaattaatt atttaaaaga acactttaaa tgtatgttag
    27961 atgcgattat tgaagatggt gttgaacttt ggggatatac ttcatggggt tgtattgatt
    28021 tggtcagtga atcaacgaaa caaatgtcaa aacgttatgg atatatttat gtggatgctg
    28081 atgattatgg aaaggggaca tataatcgat ataagaaaga tagtttttat tggtataaaa
    28141 aggtaattga aagtaatagt atagatttta aatgaccaat agaaggtgag caaataaaat
    28201 gaactacata ataaagaaag ttttgaactc gagcgttgtt ttagttcatg acacaaagga
    28261 taatgaattt attttgctag ggaaaggaat tggatatggt aaaaaaacag gagaatatat
    28321 ttgtggttcc aatgataatc aaatgtttgt ccctgttgaa aatacaaaat caaaacaatt
    28381 tttagaatta atggatgata ttcctgtcga cattttaaag ataacacagg aaataatagt
    28441 agaggcagaa aagttattga actcatcttt taataaaaac ttatacttaa tattagcaga
    28501 ccattttaac tttgcaattg aaaggatgcg taaaggaata aaaattacaa atcgagtatt
    28561 ttgggaaata aaaaattatt atccaaatga gtttaaggtt ggaatggttg caatttatat
    28621 ggtagaggag agattgggca ttcaattacc agaggaagaa gcagctaaca ttgctttcca
    28681 ttttgcaaat gctatggctt cagatggaaa ttcatatgat acgattaagt atgccaaagt
    28741 aattggagaa ataattaata tttttgtttt ttctttgaat agaaccttag ataaaaagag
    28801 tatgcattat atgagattta taacacatat aaaatttttt gtggaaagat ttttttcaga
    28861 tagtatgtta agcagtggtg atgatttatt atttaatcaa atgaaaaggt caaatccaaa
    28921 agaaatgtta attgcattaa aagttagaga ttttttagaa aagaaatatg gaaaaaagtt
    28981 aacaaatgag gaaatagctt tcttggttgt ccatatcgca cgagttaagc aatagagtga
    29041 attactaata ttatcttata cgtctacatg gtagaaaatt gaaagaaggt aaagtatata
    29101 aagaaactga gacatattat tttgttgttc attatgttta ttttatgagt gtatcctgtt
    29161 aaaaatttaa gttaatattt ataattaaaa tatacaaata gttaaaataa gtttatgtgg
    29221 ttatgaattt attttaacta tttttttgtt taatggaaaa tatgttttat aactagaatt
    29281 ttatttttaa ttaattataa tacaaaaaac tttataaaa
//
LOCUS       DAMJVI010000040        28024 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.042, whole genome shotgun sequence.
ACCESSION   DAMJVI010000040 DAMJVI010000000
VERSION     DAMJVI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.042"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            43..894
                     /locus_tag="PC545_001867"
     CDS             43..894
                     /locus_tag="PC545_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HDF2456635.1"
                     /translation="MKNIISAKELISKLEKNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNDTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEEKVNIPSCTGKEIRPNINNGLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(1027..2640)
                     /gene="ggt"
                     /locus_tag="PC545_001868"
     CDS             complement(1027..2640)
                     /gene="ggt"
                     /locus_tag="PC545_001868"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDF2456636.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLMECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSKKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELELEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPKHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVEPEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(2865..4802)
                     /locus_tag="PC545_001869"
     CDS             complement(2865..4802)
                     /locus_tag="PC545_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456637.1"
                     /translation="MTLFRIAMQNVKNSFFNYLMYFISIVFSVFVFFSFKSIEYNEAL
                     SSLGEKTRMSINTSSIVIVVFVFLFIYYSNSFFFNRRRREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLI
                     IFIVIGIRNTIVIRNKRIIELFNKYPEKYKFKKFIKLKCVLGVLLIAISYLMSISYFI
                     AENIMLSVFILVTIIPGTFLFFSSTMYMIIDAVKKRKSFYYKGQNLIAFSELGFKLKS
                     NSSVLAVIAILIATSVTMLGFTISLYYDIDRNISENYKYSYTINLGNSYVNNEIDKLL
                     YKYKKDNKIIFDKIIELIDKDINIEMYYKNRNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELTSKESVYYVSDSYKKIFFKDFEIEKINLHIKDANEYDINLFKVQKSILEPEIN
                     SQATFDLIVVKDDVFNQLKLHGNSRLIRLIDIKNEKKELDLTLKLKNIVSENMKFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDKERYIMLI
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIAQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMIKE"
     gene            complement(4789..5556)
                     /locus_tag="PC545_001870"
     CDS             complement(4789..5556)
                     /locus_tag="PC545_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456638.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRIKEISKELNIESILDKYPGEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            5844..6518
                     /locus_tag="PC545_001871"
     CDS             5844..6518
                     /locus_tag="PC545_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456639.1"
                     /translation="MFKIMVVEDDVSLKNIIAKCLTKWGHDVHQIENLENIIEEFKNY
                     NPELVLLDINLPFYDGFHWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSVDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIS"
     gene            6542..7546
                     /locus_tag="PC545_001872"
     CDS             6542..7546
                     /locus_tag="PC545_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2456640.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTVSVIIFSPVDVFLTDTILYI
                     LVVNVVFLFLYLFISYIKKNKFLDKIKNDTFKINMNDIELARCKNEEKIYLNIIKNHQ
                     YQCNNIINNNIEKFEENKEILNMWVHDVKIPIAIIKLILEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILDLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRTKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIDVDSEYNQYTKFTITFKCDL
                     "
     gene            7658..8887
                     /locus_tag="PC545_001873"
     CDS             7658..8887
                     /locus_tag="PC545_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2456641.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRNSSFGSSGFKSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTELQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENKDAIASINAQITALNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVANSSFKIPASCVLKDNKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     GDTTPNKKVEEVTMDVN"
     gene            8895..9569
                     /locus_tag="PC545_001874"
     CDS             8895..9569
                     /locus_tag="PC545_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456642.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKALKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            9572..10804
                     /locus_tag="PC545_001875"
     CDS             9572..10804
                     /locus_tag="PC545_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456643.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLVPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVIAKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIVATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(11090..12355)
                     /locus_tag="PC545_001876"
     CDS             complement(11090..12355)
                     /locus_tag="PC545_001876"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDF2456644.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMIKAKELDVPLSFHEEDPSLVGNPGVNAGKVASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSKEEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSINPAKLYNLNIGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKVNYTICNGEIVYNA"
     gene            complement(12408..13556)
                     /locus_tag="PC545_001877"
     CDS             complement(12408..13556)
                     /locus_tag="PC545_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HDF2456645.1"
                     /translation="MKSVSVRSCKTYNYEEVKLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKIHSEKAHVLKYMDIIKPIVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLLPNKVATIRGAIKRGYIQEDFSDIEILG
                     EDIKDLIIKNYKIPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPASTIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(13656..14201)
                     /locus_tag="PC545_001878"
     CDS             complement(13656..14201)
                     /locus_tag="PC545_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2456646.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRHFGK"
     gene            complement(14424..15368)
                     /locus_tag="PC545_001879"
     CDS             complement(14424..15368)
                     /locus_tag="PC545_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2456647.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVVVQTFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
     gene            15754..16635
                     /locus_tag="PC545_001880"
     CDS             15754..16635
                     /locus_tag="PC545_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2456648.1"
                     /translation="MVKKRKKKTVTLTILVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAVKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPNRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(16696..17904)
                     /locus_tag="PC545_001881"
     CDS             complement(16696..17904)
                     /locus_tag="PC545_001881"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDF2456649.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKEGSVNPRDIKMNLAREIVTLYHGEESAKEAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVSNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(17977..18229)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(18381..19370)
                     /locus_tag="PC545_001882"
     CDS             complement(18381..19370)
                     /locus_tag="PC545_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HDF2456650.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIIAVSTGFGYAIYLDF
                     IVPVFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWASQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKFLIFKNFRNLNQFLCCSKKAFYICV
                     SYLAIFEVLKLLNVKVDSVYLKTIFISITYISVYFIIRDSCMSLQNFIIAKFRKLLYA
                     RILFLIIILLLFFIFDVTVISLIIINAFLNKKINIRLSQSNIVNKQINLLIEK"
     gene            19522..19962
                     /locus_tag="PC545_001883"
     CDS             19522..19962
                     /locus_tag="PC545_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2456651.1"
                     /translation="MFKIKHFNDLSLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKS
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSSHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            20289..20516
                     /locus_tag="PC545_001884"
     CDS             20289..20516
                     /locus_tag="PC545_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2456652.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            20542..22662
                     /gene="feoB"
                     /locus_tag="PC545_001885"
     CDS             20542..22662
                     /gene="feoB"
                     /locus_tag="PC545_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2456653.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKYKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEARKKGITIDKKLLEDSLG
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(22795..23613)
                     /locus_tag="PC545_001886"
     CDS             complement(22795..23613)
                     /locus_tag="PC545_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2456654.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKFEEYLVLSSDLSNMSNSDFMMELIDFTNYCK
                     SKNLYQGYELGVIVSNENIKNGDYLSISSFYLKIDKKIKDKKLYVKPEGMYACIKHIG
                     KYEDSYKSYEKLKKYIYENEYKIVGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            23820..24104
                     /locus_tag="PC545_001887"
     CDS             23820..24104
                     /locus_tag="PC545_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456655.1"
                     /translation="MLVRVKIVIGATIRYLIKHKVNYCNKKSLDKLELKYGNIDREKA
                     VKLEIFYQYLIELEYIEMSLLIRRLDTAITSLALVSIITIASYYLIRKNV"
     repeat_region   24262..25085
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24329..24357
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(25744..27837)
                     /locus_tag="PC545_001888"
     CDS             complement(25744..27837)
                     /locus_tag="PC545_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2456656.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIIFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVLELLKNDKKGVLIKWYEKCNKLNRVFENYDSLITWQD
                     GTVVHMHQSIDIANSTSLNKPIKINEFHEISNNKEEKGVFNFSRDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLVYWKKIVHPEDWNRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGEPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLDIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGILVDNVEENEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKCASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTESHKIIGVEVLLRWTNDKCKAISPLEFIPILEENDMIN
                     IVGAWVLRMALRTFRKWIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDENFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDIVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQIKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
BASE COUNT         9877 a         4244 c         3431 g        10472 t
ORIGIN      
        1 taaattaaaa taaagataat ataaacacag gaggtacatt ctttgaaaaa cattatttca
       61 gcaaaagaac ttatatcaaa actagaaaaa aatgataatc tagttgtaat agattgcaga
      121 tttgatctta tcaatagaac ttatggtatt gattcttata aaaaaggaca catcaaaggc
      181 agctttatct tagatataga caaggattta tcatctccta caaaagaaca tggtggtaaa
      241 aatcctttac aagacccttt aatactaaaa gaaaaattag aaaaaatggg tgttgataac
      301 gacactacga ttgtaactta tgatgatgga gatttaaatg gagcttgcag attatttttc
      361 caattaaaac atttggggct taaaaatgtc tatgtactgg atgggggaat aacatctttc
      421 gtaaaggaag gtggagaatt ggaagagaaa gtgaatatac caagttgtac aggtaaggaa
      481 attagaccaa atataaacaa tggtttagtt gttccaatgg aatatgttaa gtctaaatta
      541 tacaaaaaag atactgtaat aatagattgc cgtgctaatg agagatacca gggtttagtt
      601 gaacctgctt actcaaaagc tggtcatata ccaagtgcaa aaaattattt ttgtaaggat
      661 ttaataaaaa gtgactttga aaatggctca cttaaagata tagaattttt aaaaaaattc
      721 tttaaagatt taaataatta tgatgaagta attttatctt gtggttcagg gatatctgct
      781 tgtgttaaca gtcttgctct tagagaactg gatatacctc acaagattta tattggtagc
      841 tttagtgact ggattagtta tgatgacaat gaaatcaaaa ctggtcaaga ataaggctca
      901 taatctatat aaaatcagca tatgatagct taaattttgt aaaggcgtca gtcattgtag
      961 actttcgcct ttactaatta tttcatatac tctttaaatt tattatatat attttagttg
     1021 ataatattaa taaactgcaa tatgaccatc acatcttgat tccgtcccac aaatataact
     1081 ttcctgttct gttttaaata ttatttgacc tcgtcccatg tcgacagtat catgaattac
     1141 ttttatttca tgaccctttt caattaatga atctattatg tgctttggca tctctggttc
     1201 aacttctatt tcctttcctt ttatccactg ccatcttgga gcatctagcg cctcttgtgg
     1261 atttaatcca aaatcaatca tatttgttaa cacttgaata tgtccttgtg gttgcataaa
     1321 agctcccatt acaccaaatg gacctattgc tttatcttct ttacctaaaa atccaggtat
     1381 tatagtatga taaggttttt taaatggttt tactacattg tggtgtttag ggtctaaatt
     1441 aaaattattt cctctactat gtaaagcaat cccagttctt ggtatcacaa ttcctgaacc
     1501 aaaatttatg taattgctct gtatatatga aaccatattt ccatccttat ctgcagtaca
     1561 aaggtatact gtaccaccac aatatggctc tcctgcagtt ggatacaaag cctctttatt
     1621 gtcaatcaat aaacttcttt ttttagcata ctcttggctt agtaattcct gaatctttac
     1681 cttcatatgc tctatatcag ttacatatgt tttagaatct gcaaaagcta attttaaact
     1741 ctctataatt ttatgtatgt cttctacatt ttctatattt ccttctaatt ccaattcttt
     1801 taatatgtta agtgccatta aaactgttat tccatgacca tttggtggga tttcaaatat
     1861 tttatatccc ttatattctg tacttatagg ttctacccat gttggataaa aacactctaa
     1921 gtcactcttt cttatagccc cattaaattt tttagaatat tcatctattt tatcagcaag
     1981 tcttcctcta taaaagcttt cagcctgagt atttgctatc tcttctaatg tacttgcctg
     2041 ttcttcacat ataaatatat ctccagcctc tggagcttta ccatctttag aaaatgtatc
     2101 aaaccatggt ttgaactctt ctccaataag ttctttttca tagagttcat atgatttttt
     2161 ccatacctta gctacattag gagatactac gtatccctct cttgcatagt ttatcgctgg
     2221 agataagcat tccattaagc taagtttacc atatttttta tttaattctg accaagctgc
     2281 tggtattcca ggtacagtta ctgctccaaa cccatatttt ggcatttctt tcatattatt
     2341 ataatttttt aattccatat tttctggagc aaagccacta gaatttaaac catacaatcg
     2401 tttttcttcc tctatccaaa caagggcata tgcatctcct ccaatcccat tgcttgtagg
     2461 ttctactact gctaatgtgg cggctgttgc aactgctgca tcaacagcat ttccaccctt
     2521 ttttagtatt tcaagtccag cttgagatgc taatggtgtt gatgttgcaa ctgctccatt
     2581 tttagcatac acgacatttc ttattgatga gtatttatgt tggtaggcat taaacttcat
     2641 aaatttcagc tcctcgtcat tttttatttg atattctgct tagatttatt atataacatc
     2701 aattaatgta actgatacaa aatgatataa aatatagact ttttcctaac tttaatttta
     2761 tctttaaata aatatcttaa actcaattct agtattttct atctgcactt aaataagtta
     2821 ttttattcaa aacatatatc taattctttt aacttaaaaa taatttattc ttttatcatg
     2881 tttagagcat attttaaagt taaaaaataa tatataaaat atcctataaa ataaatgact
     2941 agagttatga taattggtac aagtaatgac cttggtataa acttttgtgc tatagacata
     3001 gcaaataaat tatgaactgt acctacaact aatggcatta aaaatataac ttttaactgt
     3061 tttgatatta ttttctcaat atctttatta tttgctccaa gttttattaa cataatatat
     3121 ctttccttat catcatatat atttgataat tgtttaaata agattatact acctgtacat
     3181 aaaagaaata ctacagacaa aaatatgcct ataaacagca ttagaccact taattttatc
     3241 aagttatcat aactttcaat acttgatgta aaattaaaag gataagtaaa tttcatattt
     3301 tcacttacta tattttttaa ttttaaagtc aaatccagtt cttttttttc attttttata
     3361 tcaattaatc ttattaatct agaatttcca tgtaatttta actgattaaa tacgtcatct
     3421 tttactacaa ttaaatcaaa cgtagcttgt gaatttattt ctggctccaa gatgcttttt
     3481 tgtactttaa acagatttat atcatactca tttgcatctt taatatgcaa gtttattttt
     3541 tcaatctcaa agtctttaaa aaatattttt ttgtatgaat cagaaacata gtacacagat
     3601 tcctttgaag ttaattcttc ataattgtta ttctgatatt gtcttaattt tttaaaatca
     3661 ctttctctaa taatatctac agacgttcta tcttctatat aactatttcg atttttataa
     3721 tacatttcaa tatttatatc tttgtctata agttctatta ttttatcaaa gattatctta
     3781 ttatcttttt tatatttata taataatttg tcaatttcgt tatttacata agaatttcct
     3841 aaatttatgg tataactgta tttataattt tcacttatgt tcctatctat atcgtaataa
     3901 agtgatatgg taaaccctag catagttaca cttgtagcta ttaatattgc tattacagcc
     3961 aatacactac tattgctctt taatttaaat ccaagttcag aaaatgcaat taaattctgt
     4021 cccttataat aaaaactttt tctcttctta acagcatcaa taatcatata catagttgat
     4081 gaaaaaaata aaaaagttcc aggtattatt gtaactaata taaaaactga aagcataata
     4141 ttttcagcaa taaaataact aattgacatt aaataagaaa tagctattaa taaaacacca
     4201 agtacacatt ttaactttat aaatttttta aacttatact tttctggata tttattaaat
     4261 aactctataa ttctcttatt tctgataact atagtatttc ttattccaat aactatgaat
     4321 attattaaaa ataccgctag tgtctgaact aaggccttta tacttaagtt catatttact
     4381 actatcattt cattcattaa ttttacaagc atcatagtca taagctttga aaataacagt
     4441 cctaaaaata tacctattag tattgcaact atacccatca aaaaagtttc atataaaaat
     4501 atttttccta tctgattttt cctcattcca agtaaactat aagttcctac ttctcgccta
     4561 cgtctattga aaaaaaatga gtttgaataa tatataaata aaaatacaaa taccactatt
     4621 acaattgaac ttgtattaat actcatccta gttttctctc caagtgaact caacgcttca
     4681 ttatactcta tagatttaaa actaaaaaat acaaatacac taaatacaat tgaaataaag
     4741 tacattaaat aattaaaaaa actattcttt acattttgca ttgcaattct aaataaagtc
     4801 atttttatta actcctccaa ttaaggaaac tgtatttact attttattat agaactctct
     4861 gttactttct ccctttacaa tttctgtgaa tattattcca tcctttataa agagtattct
     4921 attgcaaaaa ctcgcactta tagcatcatg agttaccatc aatattgttt tattgctgtc
     4981 tttgtttaat tttaatagac aattcaacaa atccctagca gatttagaat ctaatgctcc
     5041 tgtaggctca tctgcaagta ctaatgatgg ttttgttgct atagctctac atacagctcc
     5101 tctttgcttt tgtccaccag aaacttcgcc agggtattta tctaatatag attctatatt
     5161 taattctttt gaaatctctt ttattctatt ttctattact gatactcttt cattttttaa
     5221 agaaagaggc aacattatat tttcttttat tgtaagtgta tccaaaagat tagaatcttg
     5281 aaatacaaag cctagattat cacgtctaaa atctgccaat tcattttgct tcattttaat
     5341 agtattcttt ccatttatat aaatatctcc tgatgaaggt aaatctattg tagaaataat
     5401 atttaaaagt gttgatttac cagcaccgga agaccccata ataccaacaa attctccttt
     5461 ataaaccttg aaagttattc ctcttagagc ttcatgtttt gaaccttttc ttccatactc
     5521 cttttttata ttttcaacag ataatatttc ttccatagtt aataatcacc tttcatcaca
     5581 attagttatt tatcaattct cacaaatata ttatatatat taaaatggct tttaaacatt
     5641 taattatatt aatttaaggt tacattttcg taagtattaa ttatatttct tcaaatttat
     5701 tgcaattatg tttaaaatat aatttatcaa gtaaaatata gttatattaa tataattatt
     5761 ttaggtaata aggtagaaat attttaattt atcaagtaga atatagttat gttaatataa
     5821 ctattttgaa ttaggaggta agaatgttta aaataatggt agttgaagat gacgtctcat
     5881 tgaaaaatat catagcaaag tgtcttacta aatggggaca tgatgttcat caaatagaaa
     5941 atcttgaaaa tataatagaa gagttcaaaa actataaccc agaattagtt ttattggaca
     6001 taaaccttcc attttatgat ggttttcatt ggtgtaatga aataagaaaa atttcaaaag
     6061 ttcctataat ttttatttct tcaagaaatt ctaatatgga tgtaataatg ggtgtaaatt
     6121 taggtgcaga tgattatata caaaaacctt tttctgtaga tgtcttagta gctaaagtta
     6181 atgccctttt aagaagaact tataattttg ttgataataa ctcaaatcag attattcaca
     6241 atggtgttac tttagattta tcaactgcaa caataaacta tgaagataat actattgagc
     6301 taacaaaaaa tgaaatcaaa attctccatg agcttatgaa atataagggg cagatagtaa
     6361 gtaggaataa actcatgaaa aaattatggg ataatgattg gtttgtagat gataatacat
     6421 taactgtcaa tgtaaataga atacgctcaa aattaaatga aattggttta gaagatttta
     6481 tagaaacaaa aagaggattg ggttatataa tcagttaaaa caattctaag taggtgaatt
     6541 tatgagcttt aaagattttc tcaaggataa actaggtttt ttagtataca attttacttt
     6601 cttaattttt acagtatcag ttattatatt ttctcctgta gatgtttttc tgactgatac
     6661 tattttatat atattagttg taaatgttgt gtttttattt ttatatctat ttattagcta
     6721 catcaagaag aacaaatttt tagataagat aaaaaatgat acattcaaaa ttaatatgaa
     6781 tgatatagaa ttagctagat gtaaaaatga agaaaaaata tacttgaaca ttattaaaaa
     6841 tcatcaatat caatgtaata acataataaa taataatatc gagaaatttg aagaaaacaa
     6901 agagatttta aatatgtggg tacatgatgt aaagatacct atagctataa taaaactcat
     6961 tcttgaacaa aatgaaaatt taattgatga aaaaatttca gatgacatag atagtgaaat
     7021 atttagaatt gaaaactccg ttgaaaaaat tttatattta tcaagactcg aagattttca
     7081 taaggatttt ttaattcaag aggtaaatat tgaaaaaatc attagaaata ttataaaaaa
     7141 atactcaaaa tattttattt caaaaaaaat tattttagac ttaaataatt tgaattttaa
     7201 ggttttatct gatgaaaaat ggctatactt catattcgag caattattga gtaattccct
     7261 aaaatactcc tcattaggag ataatatatc tatctattgc aaaagaacca aaaattattt
     7321 acaattaaga gtggaagata ctggttgtgg tataaaaaag gaagatttaa atcgaatatt
     7381 caataaggga tttacaggta ataatggaag aaacaattct aaatctacag gtcttgggct
     7441 ttatttagta aaagaacttt gtgataagct tggtcataaa attgatgtag attcagagta
     7501 caaccaatat acaaaattta ctattacatt caaatgtgat ttatagtatc ttattattga
     7561 tagtttattt tcattaactt atattcaact tcttatagta attctatatg tacatcccag
     7621 tatataatta agtagttttt ataaaaagga ggaagacatg tcttttctaa aaaaatttaa
     7681 aaataaaaga tttagaaata acagttttaa aagtaacaac tttagaaata gcagttttgg
     7741 aagtagtggc ttcaagtcta aaaataaagg ttttaaaaac aatcccttta aaaataagag
     7801 attcaaaata tatgcaggaa tagcagtaat catactttta attctaggag ttcttgctta
     7861 tgttgattcg aaaaacactg aattacaagc taatgaaaat tttatcgaaa catatactat
     7921 tcctgaaaat gaaaaaatat ttataaatgg gatggttgtt cctaagcaaa ctaaggactt
     7981 caatatatct ggagattatg agttaagtga tgttaatgtt acaaatggtc aaaaagtaaa
     8041 tcaaggagac cttcttttta ctgctaaaaa tccaactata atagcagaaa tagatagttt
     8101 aaaatcacaa cttagtcaat acaaaaaaca aaaaatatca ctttctgata ttgctgaaaa
     8161 taaagatgct attgcttcta taaatgcaca gataactgct ttaaactctc agatagcttc
     8221 tctggataaa aaagcttatg acagagttac tgctcctttt gatggcacag tatacttgaa
     8281 tgaccaaact ggaaatccag accaaccagt ttcatttatg actattcaag gtctagagtt
     8341 ttatatgaaa ggacaagcaa gtgaacaaga tttacctaaa atgaagattg accaaatggt
     8401 caatatacta gttttttcaa ctgaccaaaa gcttactggt agaataagtt ttatatctga
     8461 taaaccatct actccaaata ctgaaatggg agctcagcag aatacactat cttattatga
     8521 tataaacata gcatttgata atcaagaagg attagtaaat ggattccatc ttcaagcatc
     8581 acttgaagta gctaattcaa gctttaaaat accagcatct tgtgtactta aagacaacaa
     8641 acactcatat gtatttaagg atttagatgg tatattgaaa aagcaaattg tagatgtagc
     8701 aagtcaaaat gatgattttg ctgtagttag aggcggtctt gaacaaggcg atataataat
     8761 aaaacaccct actaaagaaa tgaaagaagg agacccagtt caaggcgatg gcgtaactgc
     8821 tggttctaat aatggagata ctactccaaa caaaaaagta gaagaagtta ctatggatgt
     8881 gaattaattt acatatgtta attaaattag aaaatattca aaaatattac aaagtaggga
     8941 aagatgaatt acatgtttta aaatccttaa atttagaaat tgaatctgga gaatttgtca
     9001 tgataatggg taagtctggt agtggtaaaa ctactttact taatatctta ggttttctag
     9061 atgtatttga tgaaggaaga tatatatttg atggaacaga tgtaactaat ttgagtgaaa
     9121 atgaacgttc tgtttttaga aatattaata ttggatttgt tttccaacaa tttaatttaa
     9181 tagaaactct aaatgtatat caaaatgtag agttgcctct aatttacaat aaagcactaa
     9241 aaaaatctaa tagagaagaa attgttaaag ataaattaag ttctgttggg cttttggaca
     9301 agcttaagca gaaaccactt caattatctg gtggtcaaca acagcgtgta gccattgcac
     9361 gttgcctagc aaatgaccct cagataatct ttgctgacga accgactggg gcattagata
     9421 gtgaaactag cagagaaatt atggagcttt taacaaggct caacaaacaa ggtaagacta
     9481 taataatggt tacacatgat caagatttaa ctaagtatgc aactaaggtt atacgactta
     9541 aagatggtgt gtttacttcg gaggtgtaat catgagttta ttaaaaaatt ctttggccaa
     9601 tttaaaaggt cacaagcttc gtgtatttgt agctttactc tggattataa taggaataac
     9661 ttcagtaata cttgttagtt ctataggtaa tggatttcaa aaagaaatta agaaatctgt
     9721 aaacaatgta aaccctaata aaactactat ttcgtttgaa tcagccgata acacaggtct
     9781 tactgatgat atgagtattt ttttaaaacc ctttaatgca aaagatttag aagaattatc
     9841 atttgttgaa ggagtagaaa gaatagctcc ttcaagagat gggtttaacc ttgattcagt
     9901 ttactcttca caagcatctt ttgataaaaa aactacgtat gttgatgttg gaccagtaaa
     9961 aaaagattct aaaataaatt taatttgtgg tagagatttt tctttagatg atgaaaaaag
    10021 aaaagtaatt ttacttacat tacaaagtac aagtgaaatt tttgaaaatc cagaagatgc
    10081 cttaggtcat ggtatcaaca taaatggaac tatatttgaa ataatagggg tattagatga
    10141 ttctcagcaa aaccaagctg gaggattctt tggaggttac caagatatgc aatttactac
    10201 ttccctagta cctaaaaagg cttttgacac tttaatgagt caaaattctt attcaaatga
    10261 aatttaccag ttagacttag tttcttctaa aggctataat gtaaatgaag ttgcaaacaa
    10321 tgttatagct aaattatatg agatgcaccc tggtatcaat ggttcttata ctacaccaga
    10381 cccaactgaa cagacagctt atcttgaaag cattaattcc aatgtaaaca aatatgtatc
    10441 tataataact gtagttgcaa tgtttgttgg tggtattggt gtaatgaata taatgtatgt
    10501 atctgttatg gaaagacaaa gagaaattgg tataagacgt gctattggag caaaacctag
    10561 gtctatatta tttcaatttt tagttgaagc tgtttttata acagtatgtg gtggaatttt
    10621 aggaactata gttggatttg ctgctactaa ctatgtttct aaatatatag gttttgaagc
    10681 tatacctagc ttaaatagct tattttatgc aattgtagct actatattga ctggagttgt
    10741 atttggtctg attccagcct ttaaagcttc taaactagac cctataaagg caatttataa
    10801 gtagaaattg tataaagatt ttcgattttt agattagttt atataaaaca tacttaaata
    10861 tataacgcac tattatatat gatagattag gaacttaatc tttccaaata taatagtgcg
    10921 ttctttcata aaaaggattc tcttccttac tatatctaaa ttaccaagat gttatgttct
    10981 aattgttcaa actaatactt ccaattttga ttaatcaatc tcattctcct aatatacaat
    11041 cttttacttt caatcattgt acactccgcc ttaaaaaaat aatcttaaat taggcgttat
    11101 atacaatctc cccattacat attgtataat taacctttcc atataaagat ttacctttaa
    11161 atggtgaatt atcagcttta gaaacaaagc tttcaactgt ccattcttct tctggattga
    11221 atataacaat gtctgctaca gccccttctt ctataaatcc aatatttaaa ttgtacaatt
    11281 ttgcaggatt tatagacatt ttttctatta actgcatcat actaagatga tttttatgca
    11341 ccaaatttgt tattccaaga gctaaagaag tttcaagccc aataattcca cttggagctt
    11401 ttataaactc tctatctttt tcttctttac tatgaggtgc atggtctgtt gctattattt
    11461 caatagtacc atcatttaat ccctctataa tcttatctct gtcccattta cttcttagag
    11521 gtggattcat ttttgcattt gttccaaact ccaatacatc ttcctcagtc aatgtaaagt
    11581 gatgtggtga agcttctgca actacatttg cacctaagta ttttgcaaat ctaaccatat
    11641 caacagatac acctgaactt atatgttgta tatcaacttt agcaccagtc tttagtgcca
    11701 acatacaatc tctggctacc ataacatctt ctgctacact tgatgcacct tttaatccaa
    11761 gttctgaagc tacctttcct gcatttacac ctggatttcc aactaaagaa gggtcttctt
    11821 catgaaaact aagtggtaca tccaactctt ttgccttaat cattgcctcc ataattagat
    11881 tgctatccat taaaggtaat ccatcatcag taaaccctgg tacaccagct tttttaagag
    11941 cttccatatc aactaactct ttaccttcaa aacctttagt tatagctgct gcttgtaaaa
    12001 catttataca tgcatttttt gacttttctt taacataatt aaaagtatct tcattatcta
    12061 caataggatt agtatttgcc atacatataa cagttgtaaa tccacctcta gcagcagatt
    12121 tagctccact ttctatatct tctttatatg taaacccagg gtctctaaag tgtacatgta
    12181 catccactaa acctggagca actacattac cactagcatc tattattttt tcatattcat
    12241 cactttcatc aaacttgcct attttataaa ttttattttc ttttataatt aagtcaatat
    12301 tctcatctct ttgactttta gggtctatta ctctaccatt tttaattaaa atcatattat
    12361 ttctccttta tatttttaat ttatatagac tcatataata ttatacatta ttttacatac
    12421 ttaaatacaa aattacgttt gacatcaata gcctttttag gacaaagttc atggcaacag
    12481 taacatctta tacatttact taagtctata atagcaccct tagtccctat ctctatagtg
    12541 gatgcaggac aagcttcctt gcattttcca cattttatgc aatcttgaaa tcttaccaca
    12601 ggctttggtg ttataagctt tgaaataggt tcatttaaaa attttggtag ctttacggaa
    12661 aacaatctta aatctttact tacatttgga atcttgtaat tttttataat taaatctttt
    12721 atatcttcac ctaaaatttc aatatctgaa aaatcttctt gtatatatcc tctcttaata
    12781 gcacctctaa ttgtagcaac tttatttgga agtaaactta taatcttaca tgcaacaaca
    12841 tctatagcat atggattttg tgatgctaat aaaactccta tttctcgagt agtaccagct
    12901 gaaggtccat caccttccat acctactata ccatccataa ttgtcaatgt tggttttata
    12961 taatcacaaa tatctaaaag tacattttcg caaaacaact cttctgtagg aaatctataa
    13021 tgtatctctg cttttttcaa tccagctatg catccatata agttttttgt accaccagta
    13081 aaagttgcca ttatatgagt ctttaattta caaagattta ttatataatc tgaatctact
    13141 attggtttaa taatatccat atattttaga acatgtgctt tttcactatg tatcttaaca
    13201 tctgaaacat cataatttaa cttaatgccc agttcatttg ctacttcttc aattccagat
    13261 gccttataaa tgctcttaag agaactttga ttatatggac caccagggct atctgctatt
    13321 gtaatatcac aatttaactc taaaagtttt tcacatacaa cttttacaac cataggatgt
    13381 gtagttgtag cttcttcagg cttttttttc attaataaat ttggttttat aagtactttg
    13441 cttccttttt taacaaactt atctatacca ccaatatcct ctaaattttt ttcaatagat
    13501 aactttactt cttcataatt gtaagtttta caactcctaa cagataccga cttcatatta
    13561 tctccatcct tctatcacaa cagtttattt atataattta aaaaaggaga aaccaaagtt
    13621 tctcctttta atctatgttt taattgtttt aattattatt ttccaaaatg tctgtttaat
    13681 aatcctaaga atgctttacc atgtctagct tcgtctttac acatttcatg tactgtatca
    13741 tgtatagcat ctaatcctaa ttctttagct ctcttagcta attttaattt tccatcagtt
    13801 gcaccatact cagcatcaac tctaactctt aagttttctt ttgtatctgc aactacaact
    13861 tctccaagaa gttctgcaaa tttagcagca tgttcagcct cttcaaaagc tattctctta
    13921 tacgcttcag ctacttctgg ataaccttct ctatcagctt gtctactcat tgctaaatac
    13981 attcctactt ctgtacactc accagtaaag ttagctctta atccttcgat tatttctgca
    14041 tctacacctt gagctactcc tattctatgt tcatcagccc aaaccatttc gcctttcatt
    14101 tcttcaaatt tatcagctcc aactttacat actggacatt gtgctggtgc agcatctcct
    14161 tcatgtatat atccacatac tgtacaaaca aattttttca taattaattt cctcccaatt
    14221 ttatataatt aaatttttat ttttaagttt aataacttgc tacaagaata tatatacccc
    14281 agactttata gcttaaacac taaaaataaa atttatttcc taattaattt tatcactttg
    14341 aaacaccagt atcaaccaag ttttgatgtc aaatttctac ataatacata atttatttga
    14401 ttaattagga aatagttgtc atattaaata ctttctaatg aagactcctc tgaatcttta
    14461 agtttcactt ttttgctctt taaaaaagat aatttagttt ctgcagttat gattgcaaca
    14521 aatatcaaga tactaccaat aaccaatttt gctgtaataa gctcctttaa aattattata
    14581 gaaaatattg taccaaatac agcctctgtg gataatatta tagctgttct agttccatca
    14641 actctttttt gacatatagt ctgaaataaa aatccaattg tagtactaaa tacagctaga
    14701 tacatagttc ccatgtaacc agataattca gcttccatct ttaattgacc tgcaaatgtt
    14761 tgaactacaa cagacattaa aaacgctact gtaaattgaa tagctgtcaa tgccattggg
    14821 ttgttatctt ttgcaaactc acttgtaaag aatatatgaa atgcaaatcc gaatgaacat
    14881 ataagtgtta gaaaatctcc aaaattaata gagaaatcag cttctaaaga aagtatccca
    14941 attcctataa gagctaccaa actacttatt attcctattt tatcaagttt tcttctataa
    15001 agtataaatc ctatgaatgg aactattaca acattagctg ctgttataaa cgcattttta
    15061 gatggtgtag tgtacataag tccaatagtt tgtactgtga atgctagaaa taaaaatatt
    15121 cctaaaatcc ctcctgcttt taataatttt ttacccatat tggcacgaat ctgtttaaaa
    15181 aacagtaaat tcataattat agctgccaag aaaaatctta aagttatgat ttgtagaggt
    15241 gtaagcccac cgtctaatgc aaattgggtt gctacaaagc cacttcccca tataattgct
    15301 ataaatatta gtcctatttc tccaaagtat tttttcaata aagctccctc ctttttattt
    15361 ttttgcaaaa ttataaaccc cctaaacagc aagagagttt ttctataagc attactataa
    15421 attaatatag taaatttttt cagtatttta aatctaaatt tacatacatt ttgagctcaa
    15481 atttatatcc actatttaac agtaatatat taatcatttt ttgtaaacac ctttttaata
    15541 aatttttata ttttttatat ttttttacct atactaaatt tttttataga tatttttaat
    15601 atatatgata ctttaatatt atgcaaataa tgtcatataa gttacacttt agttatctta
    15661 gttacaactt tgtcggttta ctttttctac tttcttcgat ataattataa tatatttttg
    15721 atacaattcg acaaaagggg gaatgctgta attatggtaa aaaaaagaaa aaagaaaact
    15781 gttacactta ctatacttgt tgtaatagtt attggtattt tttctatggc atgggcggac
    15841 atgtctagaa aaaaagaaat gaaagaaact caagaaagac ttaaagctga aagattagaa
    15901 aaagaaagaa ttgaaaaaga aaaaaatgaa cctattattg gtgcaaaaaa agaagctgta
    15961 aaagaatatg gatatgacgc aaaaatagtt tgggacaaac ttaacaaata cgactattca
    16021 aacaatggag aaaagatagt ctttcttaca tttgatgatg gaccatctac tactaatact
    16081 ccacaagttc tagatatctt gaaaagacat ggtgttagag gaactttctt tattaagggt
    16141 gattctcttg aaagaaaagg agctaatgaa attttaaaaa gaacatttga cgaaggaaat
    16201 gcaatagccc atcactctta tactcatgac tataaaaaac tatatcctaa cagaagccta
    16261 aatctcgata cattcgtaaa tgaacttaac aaaactgatg aagctatgaa aaaagtctta
    16321 ggaaagaatt tttcctcaaa tgtagttaga tgcccaggcg ggtacatgtc atggaaaaat
    16381 atggaacctc taggaaacta cttaaaagag aaaaacatgg catcaattga ttggaacgct
    16441 ttaaatgctg atgcagaagg taaaaagaaa aatgcacaag aattatttga acatgcaaag
    16501 aaatcttctg aaggcaaaga aatggttgtt cttttaatgc atgatactta tggaaaacaa
    16561 gaaacagtaa atgcattgga ccaaattata acatatttta aagacaatgg ttatcaattt
    16621 aaaattttaa tataataaaa aagcaagttt attgaatgta cattttatac tcaatagact
    16681 tgcttttact ttactctatt ttactagctc gatttttaca aattttttct ttcctatttg
    16741 aacaactaat tcactctctt ttacaatagt gcttaaatcc tctacttttt caccatttac
    16801 ctttactcca ccttgagaag ctaatcttct aacttcactc ttactcttaa caatctcatt
    16861 agaaactagt acttcgatta agtcaaatcc atcttcctta acttgaattg tttgtatatc
    16921 ttcaggtatt tgaccttttt ggaatacaga tttaaatctt tcttcagctt ctttagcaga
    16981 ttcttctcca tggtataatg ttactatttc tcttgctaag ttcatcttta tatctcttgg
    17041 atttactgaa ccctctttca actgagattc tattttatta acttcatcag gatgaacatc
    17101 tgtcactaaa ttgtagtatt taattataag ttcatcagga atttccatag atttttggta
    17161 cattattccc gcttcttcat ctattccaat atagttacct aaactcttac tcattttttc
    17221 tttaccatct aatccttcta ttaaaggcat cattattact atttgagact ccataccaaa
    17281 ttctctttgt aatgatcttc ccattagtaa attgaatctt tggtcagttc caccaagttc
    17341 tatatcagct tctaatgcta tagagtcata tgcttgcatt aatgggtaga agaactcatg
    17401 tactgatatt ggcatttgtc cttcatatct ctttttaaaa tcttctcttt ctaacattct
    17461 cgcaacagta atagttgctg ctaattttat tacatcttca aaatttaact tagctaacca
    17521 ctcgctatta aatcttacaa tagttttttc tttatctaaa actttgaaaa tttgttcttc
    17581 ataagttttt gcatttgcaa gaacttgctc tgttgttaaa gcttttctag ctttagattt
    17641 tccagttggg tctccaattt ttcctgtaaa atctccaatt attattacta tttgatgacc
    17701 taaatcttgt aattgtttca tctttctaag aactactgta tgccctaaat gtatatctgg
    17761 agcacttggg tctagaccca attttactat cataggcttt ccttcttttt ctgatttaat
    17821 aagcttttcc cttaactctt tctcatctat taaatcatct acacctttca taattattcg
    17881 catttgctcg tctatcgatt tcatttttca ttcctcctaa aatatttata taatctataa
    17941 tttttatttt tataataaaa aaagctttca tcctcttaaa aggacgaaag cattgcttac
    18001 gtgttaccac cttagttcat caatatctca cgatattaat ctcttagagt acaattacat
    18061 atactcgtgt actataacgt gtactaatcc gatatctctt aatggttgtt atctttcaga
    18121 gatagtgctc tgagatgtat tcaaactaaa tcaatttatt ccatttcacc aaactggaac
    18181 tctctgttaa ataaaatcta gccctacttc ttctcttcat agcatttata tattcagatt
    18241 atttcatcaa ttacaagaaa tttttcttaa cttactcata atatattatt atttttatat
    18301 tttgtcaacc ttttttatta ttatatacat gtttttttac attatactac ttttttatat
    18361 tttttaccaa tacccaaaat ttatttttca atcaaaagat ttatttgttt attgactata
    18421 ttactctgac tcaatcttat atttattttc ttatttaaaa atgcgtttat aattattaaa
    18481 ctaataactg ttacatcaaa tataaaaaac agcaataaaa ttattatcaa aaataaaatt
    18541 ctagcatata ataattttct aaacttagct attataaagt tttgtagtga catacatgag
    18601 tctctaatga taaaatagac acttatatag gttattgata taaagattgt ttttaaataa
    18661 acagaatcaa ctttaacatt caacagtttt agaacttcaa aaatggctag ataagaaaca
    18721 catatgtaaa aagctttttt tgagcaacat aaaaattggt ttaaatttct aaagttctta
    18781 aatattaaaa atttttttaa agaattagaa tctaaaatat gcaatttatt ccctaaaatt
    18841 aaacttaata aatatatgca aaacaccata ccaaaaggaa acaatgctat cattaaataa
    18901 taaataaaat ttgtataccc attaaaataa atccatttgc tatatccttg aaattctttc
    18961 ataaaactat aacctattaa tgttgaagca tatatatcaa tgaaaaccat tacaataacc
    19021 ccaaatactg aaccatatac taaatcatcc ataaccattg taggcttatt tattagatag
    19081 ccacacatta taccaagtaa tacactctga cttgcccaaa tagcagttcc tagatttcca
    19141 agtaccagag aaattattaa aagcgaagta attccagata aaatggtata ctttaaatca
    19201 catttcaagc agattataca aaaaaatact ggcactataa aatctaagta aatagcatat
    19261 ccaaatccag tactcacagc tataattgtt actactatga ataaagatga caataaagct
    19321 gcttcagtta attttttagt agtattcaaa acactgcctc cataaatcat aaattttcta
    19381 actaaagttt tgtttttaat atagtatatc actttttaaa aatattttta ataaatataa
    19441 attttaataa aggtatttat gttaaaaatg gtataatgta tcttaatatt cagtttttta
    19501 agatgctagg aggtaattac tatgtttaaa attaaacact ttaatgattt gagtttagat
    19561 gaattttatg aaatagcaaa atcaagatat gaggtatttg cttgtgaaca gaagatattt
    19621 tcattaaacg attatgatga tatagataaa agctcttatc acatattttt aaaagaaaat
    19681 ggattgattt gtgcttacgc tagaataatt cctaaagaat acagctctta taatgatgtt
    19741 tccattggta gagttttagt tctttctagt catagaagaa aaggattagc aaaacaaatg
    19801 atggattgtg ctatagattt tataaaagtt aacttacacg aaaataatat aaccttatca
    19861 gcacaaactt atataaagaa cctttactta tcttgtggtt ttaaagaaat ctcagaagta
    19921 tacgacgaag caggtattga acatattaaa atgagattat agtgttgaca agctaatatt
    19981 gttaatatat aatttaagta taaattaaat attgaattga tatttaggta tttgaaaata
    20041 ccttacatag agtcagaagg cttttgcttt ctgacttttt tattatcaaa attcaataat
    20101 tatatggatt caaagtgtta atttttattc tatattctac tttccccaaa agtaagaatt
    20161 atacccaact ttgtatgata ggcatcttgc ctatcttttt tttgtaaaag taacaaggta
    20221 ataatataat gcatagttta aaataagaag attagcccag gctaaatttc aagcatggag
    20281 gtgctattat gaaaaacctt aacgatattc aagtgaaaaa aactgtcaaa gttgaagata
    20341 tattatctag tggtaattta agagaaagaa tgttagcgct tggtcttact agaggagctg
    20401 ttatagatgt agttagaaaa ggtccaaaaa ataatttaac tgtctataaa atcagaggta
    20461 gcaagatagc tctacgacaa gaagaaagta gcttaatatt agtttctgac atttaaaaat
    20521 ttctagcagg aggatattaa tatggggttg acacataact caactaaaat gagttcttta
    20581 aaagacatgt ttgatataga caataaggaa gaccagtttg taattgcatt ggctggcaat
    20641 ccaaatacag gtaaaagtac tgtatttaat catcttacag gacttagaca gcatactgga
    20701 aactggccag gaaaaactgt agctacagct cgtggtaatt ttaaatataa aaataccgaa
    20761 tatgcattaa tagatttacc aggaacatat tctttatttg ccttatcaca agaagaaatt
    20821 gtagctcgtg attttatatg ttttggtaat ccagatgcag taatagtagt atgtgatgct
    20881 acatgtcttg aaagaaattt aaatttagta tttcaagtga tggaacttac agataaagtt
    20941 attttgtgta ttaatttaat agatgaagct agaaaaaaag gcattactat tgataaaaaa
    21001 cttttagagg attctcttgg tattccagtt attttaactg ctgcaagaaa tggctctggt
    21061 atggatgaac tattggatac tttaaatgat gtatcttttg acaaatataa attaaataat
    21121 aaacctgtta gatacaatga aaatatagag aatgtggtaa aatcaattca accagaattg
    21181 gataatatta taccaggtat aaattctaga tggttgggac ttagacttat tgatggcgat
    21241 gaaagtatat ttgaatctat gagtaattat atagacaaag attctataga tgctattaat
    21301 gaagtaaaga aaaagattcc agacaatata aataaacaga aaataagaga tgagtttact
    21361 aaaatcaact atgattatgc taaaaaactg agtgatgagt gttgttctaa tgttgctaaa
    21421 aaatcaactg atagagaaga aaaagtagat aaaatattaa catctaaaat atttgggctt
    21481 ccaataatgc tcttattatt aggtactata ttgtggatta ctatagaagg agctaactat
    21541 ccatccactt tattatctaa tttattactc ggatttgagc caagtatttc gggtatatta
    21601 aatagtatta attgtccatc atggttaaat gatatgttgg ttttaggatt atacagaaca
    21661 ttagcatggg taatctctgt aatgctacct cctatggcaa tattttttcc attatttacc
    21721 ctacttgagg attttggata tttgccaaga gttgcattta acttagacca cctatttaaa
    21781 aaagcttgtg cacatggaaa acagtgtttg actatgtgta tgggatttgg atgtaatgca
    21841 gctggagtta ttggttgtag gataattgac tctccaaggg aaagacttat agctatactt
    21901 acaaacaact ttgttccttg taatggtcgt tttcctactt tgatagctat atctacaata
    21961 ttttttagct cagttataac caactctttc gtatcaagcg tagcaactgc actttgcata
    22021 actttactga taattctagg tgttataata acattgttgg tttcttatac tctttctaaa
    22081 acgctattaa aaggagttcc gtctacattt acattagaac ttcctcctta tagagttcct
    22141 caaataggaa gaactttata tacctctata atagatagaa caatatttgt tttaggaagg
    22201 gctgtaatgg tagccatacc tgctggtgtc ataacttgga tatttgcaaa tatatatata
    22261 ggagatttaa gtatattatc acatgttgca aactttttag accctttagc taaattaatt
    22321 ggtcttgatg gttttatatt acttgctttt attcttggat tcccagcaaa tgagatagtt
    22381 gtacctatac ttttaatggc ttacctagca actggttcta tgatagaatt agatagcttt
    22441 agtgctttag gtcaagttct tagagaacat ggatggactt atttgacagc tttaaatgtt
    22501 atgctgttca gtttactgca ttggccttgt gcaacaacac ttttaacaat aaaaaaagaa
    22561 actggtagtt taaaatggac tgccttagga ttcttaatgc ctactatact tgcttttgtt
    22621 gtttgttttt taacaactac agtttacaat ttatttatat aattagtatt aactgacagt
    22681 agacatatat tagagaaacc atttaaaaat ttatatactt tattataaag catagagata
    22741 tattcaatga taagtaaaat tacttaacat ttgaatatat ctctatttta attcttatct
    22801 ggatacttgg attgaaattt cagtcatata attatcttca ttactttcac aaaaaaagtc
    22861 aagtatactc tcctcatacg aatttccaac aattttatat tcattttcat atatatattt
    22921 tttcaatttt tcgtaagatt tatatgaatc ctcatatttt ccaatatgtt ttatgcaagc
    22981 atacattcct tctggcttta catatagctt tttatccttt atttttttat ctatcttcaa
    23041 ataaaaactt gatatactta aatagtcccc attttttata ttttcattac ttactattac
    23101 tcccaactca tatccttgat acaaattttt cgatttacaa taattggtaa aatctattaa
    23161 ttccatcata aaatcactat tactcatatt actcaaatcg cttgatagaa ctaaatattc
    23221 ttcaaacttt ttggatatat aaatttcttc actattcatt ttagaaattc cttcaactgt
    23281 tatatttatt ctattttgca tcttagaact tatctttctt agttttttta ttttttctct
    23341 tatctttttt tcttcttctt taagcatttt cacacaaagc tggggagttc tattttgtat
    23401 atactctttt atttcctcta aactcattcc tatttctctt aatgattgta atatatcaag
    23461 tgtatcatat tgataaactg agtaatatct ataattattt ttatctttat attcaggaga
    23521 aaaaatacct attttgtcat agaaaattaa tgtctgctta gatataccac acaatttagc
    23581 aaattcacct gttgtaaaat actgtttttc caagggttat acactccttt tctttttgtt
    23641 gtctatatag ttactatatt ctttattatt tactaaatca agtagttatt attttagaaa
    23701 atcaatatgg ttttgatttt aaagcttaaa tatcagttag atgtcagtta taaatatttt
    23761 acccattccc atcataaaaa agatatcccc tcttaacttt tggatggaat attatattta
    23821 tacttgttag agtgaaaatt gtaataggtg ctacaataag atatttaatt aaacataaag
    23881 ttaattattg taataaaaaa tctttagata aattagagtt aaagtatgga aatatagata
    23941 gagaaaaagc tgttaaacta gaaatatttt atcaatattt aatagaactt gaatatatag
    24001 aaatgagttt attaataaga agattagata ctgcaataac atcattagca ttagtatcta
    24061 ttataacaat agcatcatat tatttaatca gaaaaaatgt gtaattatgt aagctattta
    24121 taataaaaca ctgataaaat taataataaa aagataatag gatttctata ctaccttttt
    24181 gtttttataa tatagtgtaa ttttattata tttttgtatg ctaatataac tagataatca
    24241 taagataaca cattaaaaga aaattatatt ctgtatagtt agtataaaac gtaagtaata
    24301 taaatttatg taaagcaaca aaagatggat ttacattcca tatagttaat ataaaacgat
    24361 ttcaaatgat tagaataaaa atatcagaat tgctatttat attccatata gttaatataa
    24421 aactataaag tgttactttt atttatttta ttttttacat aatttacatt ccatatagtt
    24481 aatataaaac gcattatatg gctgtgtcat tttgcattct attacatatt tacattccat
    24541 atagttaata taaaacctgc ttaatgcaga attaagagag gaagtgttat aagatttaca
    24601 ttccatatag ttaatataaa actggttgtt cctgctccgg tgttggtgga actggtacat
    24661 ttacattcca tatagttaat ataaaacgaa tatgtatgga taatttttta cttaacatat
    24721 tagcatttac attccatata gttaatataa aacctgctgt aatttctgat gaagcttctc
    24781 cactaccacc atttacattc catatagtta atataaaacc cttctttaac tcttatcgta
    24841 tttaaacctc ttataacatt tacattccat atagttaata taaaactttt tgtgttaaga
    24901 agaaaaaaga aattttgata atatttacat tccatatagt taatataaaa ccttaaagaa
    24961 aaaatggagg aagattaagt gaaaaaatta tttacattcc atatagttaa tataaaactg
    25021 gatttaaata atcaaaacaa ctccggttta aaaaacattt acattccata tagttaatat
    25081 aaaaccccaa aataaactta gtattttcaa cacttacacg tatacaactc tcttaaattt
    25141 gcagtgaacg agcaatagtg caattgataa cgcttatcat acactctcaa cgccttatat
    25201 ttcaactgtt acgccaaatt ttttcacaaa tatcgctcac tgcaaaattc ctataatttt
    25261 attatatcat aaatatatta cttttgaata ttgataccaa tttataccat aataaaagca
    25321 aaaaaacgta atctatctac attagattta aattctataa cttaaagttt tttacataat
    25381 atttatgctt gtttaatcaa tctaatatat cattcttgta tattgctttt aatagtcttt
    25441 tttcttaaat aaatatctat ctgaacaagc atagtttaat taaaaacttt tgtattccct
    25501 taaaactgta tctacctctt attattttat ttatgatata ttatttttaa tctaaaaata
    25561 gatatctatt tttattatgg ttatcccttt ttaaaatact ttaatcatat actattccca
    25621 atttaacatc cattgaaatg ctattttctt atattgcaat ttataattga tatatcttca
    25681 atataataat ttaatgtatt tttgatttta aagcttttca gtgatattat tgataaatta
    25741 gaattaatta tctagcttta aaaggtcaaa gatttctgtt gctgtctgcg gttttccaaa
    25801 taaatatcct tgtatgtaat ctggctttat ttgtttaact aaatcatact cttcttgtgt
    25861 ttctacacct tctaaacata cttttattcc tacatcatga caaattgcaa caataaaatg
    25921 tataaatgta gcatcaaact tactctttaa tatatcttta acaaatactc tatcaatttt
    25981 cactatatct attggagaaa attttaaaac ttctagagac gagtatccag taccaaaatc
    26041 atccatagca atataaattc ctaaatcttg taaagcctta aatttaaact gtaaaatact
    26101 catattttgt actgtatgac tttctgtcaa ttctaaaaca agattttgat aaggaaaatt
    26161 ttcatcatct ataattttta ctatatcttc aataaatgtg tcttctaata tttgaacagc
    26221 tgaaacgttt acactcactt taaaaaaagg atagtaatca atccatttcc taaaagtacg
    26281 taatgccatt cgaagcaccc aagcccctac aatatttatc atatcatttt cttctaatat
    26341 tggaataaat tccaaaggag aaattgcttt gcatttgtca ttagtccacc ttaaaagaac
    26401 ttctacacct attattttat gactttctgt atctacttgt ggctggaatc gaagactaaa
    26461 ccccctaaaa tcatcattaa ttgatgcctt taaatcccgc atcatttcta atgaatacat
    26521 tttatttttt aatatttctt gggagaaaaa aacaagcctg tttttaccat gctctttcgc
    26581 atattgtaaa gagtaactag cacacttata aagttctttt actgtatctc catgttttgg
    26641 atatattaca caaccagcag aaattgttat atttaatcca tattttctcc atagttgaag
    26701 gtgtataatc atattctgta tttgattata tagagttaga atttcatttt cttcaacatt
    26761 atcaacaaga atgcccattt cgtccccatc aagtttataa agttcagcat tatctggtaa
    26821 aatagattgt atagactgtg ctagtgtttt tattatatta tctccaaaat ccctatcata
    26881 catctcatta acatttttaa aatcgtcaat atctaataat actatgcata atttttcaat
    26941 cataggattt gaaattttat cttcaaatac actcataaat tcatgataat tcagtaactg
    27001 tgtcaatgaa tcaatcttat tttgtttacc taattgcgtc attattcctg caaaaatact
    27061 tggttctcca aattcatcac gcattagttg acctctacat cttaaccata tatattctcc
    27121 actacgattt ttagccctaa attccactgt atgataatcc atctggtttt tacctatttc
    27181 agtgtttgac ttataaaaac gattccagtc ttctggatgt acaatttttt tccagtaaac
    27241 aagcggattt tcaacaattt ctcctggtaa gtcaaataat tcaacttgtg atggtgaata
    27301 tcgaaatacg cctgttttca tattgcaaat gtatatatat tcatctgtac ctctaatcaa
    27361 agcatcataa agcaatgtag aattataatc aaaattgtct cttgaaaaat taaaaactcc
    27421 tttttcttct ttattatttg atatttcatg aaattcatta atcttaattg gtttatttaa
    27481 gcttgttgag tttgcaatat ctattgattg atgcatatga acaactgtgc catcttgcca
    27541 tgtaattaaa ctatcataat tttcaaaaac acgatttaac ttattacatt tttcatacca
    27601 ctttattaat actccttttt tatcattctt taataattct aaaactttac aaaaactaca
    27661 agtagaattt ttttcaggat ataatacttg ccaacaaact tttccttctg gatctaaaat
    27721 attatattct tctttcattt ttttgttcat aaaaataatt tcattagtat gaatatttgt
    27781 aacatatata ttaatctgta gttgatttaa tataacttca aaattatgtt tgttcatata
    27841 cttatctcct ccatagttac tgctctttat aacaacattg ttaaaattat tctttttaga
    27901 agatttactt ataacttata catatacact aaatacctta atttaattag aaagtttatt
    27961 acttcatatt ttaaattata attgataaaa aaaaatacag caatatagta ttaccaattt
    28021 aaaa
//
LOCUS       DAMJVI010000041        27760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.043, whole genome shotgun sequence.
ACCESSION   DAMJVI010000041 DAMJVI010000000
VERSION     DAMJVI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.043"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..180)
                     /locus_tag="PC545_001889"
     CDS             complement(<1..180)
                     /locus_tag="PC545_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2456657.1"
                     /translation="MNREKLVAFIRYLSLFILVVSYLKNVENTLYPVITMIILFLIII
                     NNQVRFFSLSNENKFI"
     gene            364..708
                     /locus_tag="PC545_001890"
     CDS             364..708
                     /locus_tag="PC545_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDF2456658.1"
                     /translation="MNNKEKGDFGEKVAVNYLLSKGAKILEKNYRLKIGEIDIIAKME
                     DEIIFVEVKSRSNIKFGYPCESVSFKKRKKIIGVANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIMNAF"
     gene            739..2340
                     /locus_tag="PC545_001891"
     CDS             739..2340
                     /locus_tag="PC545_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDF2456659.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDVSNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQNIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLDDKSRKENEELEKDNSYIDCKYTKINNEESKYDEDF
                     KDVKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISVEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLVAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFK"
     gene            2359..3459
                     /gene="dprA"
                     /locus_tag="PC545_001892"
     CDS             2359..3459
                     /gene="dprA"
                     /locus_tag="PC545_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDF2456660.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICKYDKEYPENLKNIYNAPKLLF
                     YKGDIGLVNNNFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            3492..5579
                     /gene="topA"
                     /locus_tag="PC545_001893"
     CDS             3492..5579
                     /gene="topA"
                     /locus_tag="PC545_001893"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDF2456661.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELENEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDTKKVQDAHEAIRPTSVDRTPESIKASLSKDQYKLYNLIWRRFVASQMEDSVF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            5744..6529
                     /gene="codY"
                     /locus_tag="PC545_001894"
     CDS             5744..6529
                     /gene="codY"
                     /locus_tag="PC545_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDF2456662.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDVQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            6636..7901
                     /locus_tag="PC545_001895"
     CDS             6636..7901
                     /locus_tag="PC545_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDF2456663.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKSFYKINATNSSLEDIKKVISELGSINNVNGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKLDVEKGL
                     KRAVEVLNEDSYMDIECNNNAIEDIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDVIRASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDSHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSIYYIPQENKTEKNIKKYLEYLNSDF"
     gene            7932..8702
                     /locus_tag="PC545_001896"
     CDS             7932..8702
                     /locus_tag="PC545_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2456664.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADVGEHGEIKAILLKYFEFMIFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRAKGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            8811..9257
                     /locus_tag="PC545_001897"
     CDS             8811..9257
                     /locus_tag="PC545_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDF2456665.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            9275..10468
                     /gene="nifS"
                     /locus_tag="PC545_001898"
     CDS             9275..10468
                     /gene="nifS"
                     /locus_tag="PC545_001898"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HDF2456666.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLDNRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            10522..10962
                     /gene="nifU"
                     /locus_tag="PC545_001899"
     CDS             10522..10962
                     /gene="nifU"
                     /locus_tag="PC545_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HDF2456667.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAQKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            11251..12330
                     /gene="mnmA"
                     /locus_tag="PC545_001900"
     CDS             11251..12330
                     /gene="mnmA"
                     /locus_tag="PC545_001900"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDF2456668.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDVVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNED
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGEDTIKIVFDEAQRA
                     ITKGQSVVMYDGDIVVGGGIIEKSL"
     misc_binding    12473..12711
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            12779..15418
                     /gene="alaS"
                     /locus_tag="PC545_001901"
     CDS             12779..15418
                     /gene="alaS"
                     /locus_tag="PC545_001901"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDF2456669.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVRLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAEEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGRISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            15443..15700
                     /locus_tag="PC545_001902"
     CDS             15443..15700
                     /locus_tag="PC545_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDF2456670.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            15710..17881
                     /locus_tag="PC545_001903"
     CDS             15710..17881
                     /locus_tag="PC545_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456671.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSCASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGQKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYDTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILGTFGLE
                     NKEMVGRSYTLVNKEDNISYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKDNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLLVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAVLLAVSAVVVVFAFAALDILSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            18087..18512
                     /gene="ruvX"
                     /locus_tag="PC545_001904"
     CDS             18087..18512
                     /gene="ruvX"
                     /locus_tag="PC545_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDF2456672.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKAETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            18590..18877
                     /locus_tag="PC545_001905"
     CDS             18590..18877
                     /locus_tag="PC545_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDF2456673.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            19159..19617
                     /locus_tag="PC545_001906"
     CDS             19159..19617
                     /locus_tag="PC545_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2456674.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(19734..20471)
                     /locus_tag="PC545_001907"
     CDS             complement(19734..20471)
                     /locus_tag="PC545_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2456675.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTIGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKL"
     gene            20688..22364
                     /locus_tag="PC545_001908"
     CDS             20688..22364
                     /locus_tag="PC545_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDF2456676.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGVGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDICKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            22572..22772
                     /locus_tag="PC545_001909"
     CDS             22572..22772
                     /locus_tag="PC545_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HDF2456677.1"
                     /translation="MDDVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            22826..23989
                     /locus_tag="PC545_001910"
     CDS             22826..23989
                     /locus_tag="PC545_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2456678.1"
                     /translation="MKKLASTALAILIALTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGKIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLT
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELINTKDINKASYLQMLQRIIDNMI"
     gene            complement(24200..24622)
                     /locus_tag="PC545_001911"
     CDS             complement(24200..24622)
                     /locus_tag="PC545_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HDF2456679.1"
                     /translation="MENKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFNDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(24643..25479)
                     /locus_tag="PC545_001912"
     CDS             complement(24643..25479)
                     /locus_tag="PC545_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2456680.1"
                     /translation="MRQEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPKFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLQ
                     SNLENTNIEIQAVCDYLENNYAEHIILDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPIDAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            25777..26373
                     /locus_tag="PC545_001913"
     CDS             25777..26373
                     /locus_tag="PC545_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HDF2456681.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYLY
                     KFESMSYIILLIALFTGMYRIILTPIYAVLLKIVYLFV"
     gene            26403..27122
                     /locus_tag="PC545_001914"
     CDS             26403..27122
                     /locus_tag="PC545_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDF2456682.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKEKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            27124..27663
                     /gene="scpB"
                     /locus_tag="PC545_001915"
     CDS             27124..27663
                     /gene="scpB"
                     /locus_tag="PC545_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDF2456683.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIINKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVATNE"
BASE COUNT        11247 a         2905 c         4998 g         8610 t
ORIGIN      
        1 tataaattta ttctcatttg ataaagaaaa aaatctaact tgattattta ttataattag
       61 aaataatatt atcatagtta tgaccggata caatgtattt tcaacatttt tcaaatagct
      121 tacaactaat ataaataaag ataaatatcg tataaaagca actaattttt ctctgttcat
      181 taaaggcact cctccaaaaa aataaacttt tactatgaaa actagaataa tatcataaaa
      241 gaataaagtc aataaaagta tttattaata ttgatggaaa taaaatcaat ccaaaaactt
      301 gaatagtaaa attgtatact gacaataatt taaaagtgaa aatttaatgt attggagtga
      361 ataatgaata ataaagaaaa aggtgatttt ggagaaaaag tagcagttaa ttatttgcta
      421 tcaaaaggag caaagatact agaaaaaaat tacagattaa agatagggga aatagatata
      481 atagcaaaaa tggaagatga aattatattt gtggaggtga aatcaaggtc taacataaag
      541 tttggctacc cttgtgaatc tgtaagcttc aaaaaaagaa aaaagataat tggagttgca
      601 aattattata ttataaaaaa taacttaaat aacactcccg taagatttga tgttatagag
      661 gtatatttat tagaaaaaag aataaatcac ataatgaatg ctttttaaaa aagataatgt
      721 ttttggaaag gattaaatat gttaagtatt attaattcaa gcaacttagt aggaatcgac
      781 agctttttag ttaaggttga agtagatgta agcaatggaa tacctagttt taatattgtt
      841 ggtcttccag gcaaagaaat taaagaagca agagaaagag taaagtcagc aattcttaat
      901 agtggatata aatttccaag cacgagaata gttgtaaatt tatcaccagc agatataaaa
      961 aaagaaggag cttttttaga tttatctata agtataggcc ttttaagaga gttaataaaa
     1021 aaagatgaaa attacataag agaaagtatg tttataggag aattatcatt agatgggaaa
     1081 ataagaaaag taagaggtat attgcccata attatgggtg caaaaactca gaacataaaa
     1141 cgtattttta taccaattga gaacataaaa gaaagtctat ttgtagatga aattgatata
     1201 attcctatta aaagtttaaa agaatgtgtc gattttttaa acgaagagat aaaagtcgat
     1261 aaagtaagga taatgtcttt tttggatgat aagtcaagaa aagagaacga agaattagaa
     1321 aaagataata gttatataga ttgcaaatat actaagatta ataatgaaga aagtaaatat
     1381 gatgaagact ttaaagatgt aaaaggaaat tattttgtaa agaggagtgc agaaattgct
     1441 gctgctggaa atcataatat gtttatgatt ggacctccag gctcaggaaa aactatgatt
     1501 gcaaaaagag taaggacaat tttaccagat ataagtgtag aagaaatgat agaagtaagt
     1561 aaggtctata gtatattggg tatgataaat gaaactaaag gaataattga taaaagacct
     1621 tttagagcac ctcatcatac aactacaaag caatctttaa ttggaggagg aatggatgca
     1681 agacctggag aaattgcttt agcacataga ggtatcttat ttttagatga gatagccgaa
     1741 tttgatagaa aaatacttga gactttaaga caacctatag aagatggata tgtaaatata
     1801 tccagagtaa agtacagtgc aaaatatcct tgtagagtac tgttagttgc agctatgaat
     1861 ccatgtccat gtggttatta tatgtcagag acagagtgta gatgtagaag taacgaaatt
     1921 gatagataca taaataaaat atcaggtcca ttgttagata ggtttgatat ttttgtagaa
     1981 gttaattcta taaaatattc agattttaat tctttaaaac aagaagaaag ctctcaaaaa
     2041 ataaagagaa gagtagaaaa tgctagaaaa atacagataa atagatttaa gaaggataat
     2101 ataaaaaata atagtgaaat taaagcttac aatttattta aatattgtaa actggaaaaa
     2161 gaagcttcaa aaacggctga gatgatattt aataaataca acctaagcag tagaagttat
     2221 acaaaacttt taaaaatggc aagaacaata gcagatttag aagaaagaga cttgataaat
     2281 tcacagtgta ttatagaagc tttttcattt agaaaggctt attatagtta ttttaaatag
     2341 ttttttgagg tggattttat ggaaaaaaga gatacttatt tatggttaaa atcaattggt
     2401 ggaataacaa caaaaacaat tgaaataata gaaaatgaaa tagtaaatat tgaagatata
     2461 tttgattttt ctgaaaaaga aatatataat ttaaaaaata taagcttgaa tattagaaaa
     2521 aatatagtaa aatatagagg tcacgcttat ttagaaaata taaaagaact actttacaaa
     2581 aaagcgatta aatatatttg taaatatgat aaagaatatc ctgaaaattt aaagaatata
     2641 tacaacgctc caaagttatt attctacaaa ggtgacatag gcttagttaa taataatttt
     2701 aatatagcta tagtaggttc aagaaaacct acagcctatg gaattaattg tgcaaaaaca
     2761 ataagttgtc aactatccca atatggagtt aatatagtaa gtggattagc tataggaata
     2821 gatgcatact cgcacatagg ttgtatgagt ggaaaatcta aaactatagc agtacttggt
     2881 tcaggagtag acaatcctct tccaaaacaa aatttacatt tatcaaataa aatattagaa
     2941 aatgggggat tattattatc agagtataat attaactcga cagtagcccc gtaccatttt
     3001 tctaatagaa atagaattat cagtggttta agtgatggag tagttgttgt agaagcagca
     3061 ataaaaagtg gtgcactaat cacagtggat tttgctcttg aacatggaaa aaatgttttt
     3121 gcaatacctg gtaatataaa ttctcaaatg agtagaggtt gtcataaaat tataaaagaa
     3181 ggtgcaaaat taattgaaaa tatagatgat attttaaatg aatataacat atttaatatt
     3241 attgataaaa aaataaatca aaaatatgat aatataagtc taaatgccaa gtctaagcaa
     3301 ataattgaag ctataaaaag agaaggaaac ttgcatatag atagtatttg tgattatact
     3361 ggtatagaaa taaagtatgt aaattcaata atcaatgaat tagtactaaa tgagttagta
     3421 gtagaaatga ataataaaac gtacagttta aatgtataaa ttaagattag gtagtggggg
     3481 tgtatggttt tatggccaaa acattagtca tcgtagaatc gcctgcaaaa gctaaaacta
     3541 tagaaaaatt tttagggaaa agtcattaca cagttaaagc atcagttggt catgtcagag
     3601 atttacctaa aagtaaatta ggtgtagata tagaaaataa ttttgaacct caatatataa
     3661 atataagagg aaaaggcgat gtaataaaag aattaaaaaa agaagctaaa aaatctaaac
     3721 aagtatatct agcaactgac cctgatagag aaggtgaagc tatatcatgg catttagctc
     3781 atatactaaa tttagatgaa tctgaggatt gtagaataga gtttaatgag ataactaagg
     3841 atgcaattaa aaaagcaatt aaacatccta gaaatattga tataaatttg gttgatgcac
     3901 aacaggcaag aagagtgcta gacagacttt taggttatca aataagtcct atattatggc
     3961 aaaaggttag aaaaggtctt agtgcaggta gagtgcaatc tgttacaaca aagttaattt
     4021 gtgacagaga aaaagaaata aaggcattta ttccaaaaga atactggaca atagatgttg
     4081 aggctaagac agtaaaagac aaagatataa ctttgaaatt ttatggtaaa aatgatgaaa
     4141 agattgaatt agaaaatgaa gaagctgtaa atgaaatact aaaagaaatg gaaggtaaag
     4201 atttaaaggt tgagaagata gaatctaaat caagacgtaa atcagctcca aaaccattta
     4261 caacaagtat gcttcaacaa gaaggagcaa ataagttatt ttttactact aaaaaaacaa
     4321 tgatgatagc acaagaatta tatgaaggta tcgacataga aggtgaaggt actgttggtc
     4381 ttatatcata tataagaact gactctaaaa gaatatctga tgaagcaaaa gaaaaagcta
     4441 agggctatat cgtaaacaat ttaggagaaa actactataa aaacactggt gataaaaaag
     4501 aaaaaaagga tactaaaaag gttcaagatg cccatgaggc aataagacca acctcagttg
     4561 atagaactcc tgagagtata aaagcctcat tgagcaaaga ccagtacaag ttatacaatt
     4621 taatttggag aagatttgta gctagtcaaa tggaagattc agtgtttgat atactaaatg
     4681 tagaatgtaa aataggcgat attgtattta aggcaactgg ttcaaaaatg aaatttgatg
     4741 gttataccaa ggtatataat ttttcagaaa gagaagacaa aattttacca agtatagaaa
     4801 aaggagatat attaaatata gaaaatatat tgccagacca acattttaca caaccaccag
     4861 caagatatac agaagctagt ttagtaaaaa cactagaaga actaggtata ggtagaccaa
     4921 gtacgtatgc tccaacaata gctactatac tgaatagaga gtatgttgaa aagcaaggaa
     4981 caagtctatg tccaacagaa cttggaataa tagttactga tattttggaa aataatttcc
     5041 ataaatttat agatgtagat tttactgctg atatggaaag taaacttgat gttatagaag
     5101 aaggcaatac agattggaaa aagattgtat cagaatcata tgcaccactt aaagaggcta
     5161 ttgaagaagc tcttgaaaat atagaaaaag taaatatgga tgaagaaact gatgagattt
     5221 gcgagaactg tgggtctaat atggttatca aatatggtag atttggaaag tttatggctt
     5281 gtaaaaacta tcccgattgt aaaacaacta gaccaattat gaataaaatt ggtgttaaat
     5341 gtccaaaatg tgaagaagga gatattatac ttagaaaatc taaaagaggt aaagcttttt
     5401 atgggtgttc taattatcca aactgtgact ttgtagcttg gaataagcca acaggagagt
     5461 tttgtgagaa gtgtggttca tatatggttg aaaaagtgac taaatctgaa acaaaaacag
     5521 tgtgttctaa taaagaatgt aataaagaga caaaagagca taatgacgga aaagagtgaa
     5581 aattgaaaaa aatcacataa ttctcttgaa aaaggttcca atctatgata atctttcaag
     5641 tgtaaacaac taatagaaaa ataaattttc attaaatgtg aaaataatat tagaaaatat
     5701 aaaaggttat taaaaggcct aaatttcatg aggagatgat taaatggcaa gtgaagtgtt
     5761 acaaaaaaca aggaaaataa ataaaacatt acaaacaagt ggtggaagca gtgtctcttt
     5821 tgatttactg gccggagcat tgggcgacgt tttaagttct aatgtttatg tagtaagtgc
     5881 aaaaggtaaa gtactaggtc ttcatttaaa tgatgttcaa gacagttcag ttatagaaga
     5941 tgagtatact aagcaaaaga aattttcaga tgaatatact caaaatgtgt taaaaattga
     6001 tgaaacatta gaaaatttaa atggtgagaa gatattagaa atctttcctg aagaacatgg
     6061 aagattacaa aaatatacta cagtagttcc aatattagga agcggtcaaa gattaggaac
     6121 attggtactt tcaagatatt caaattcatt caatgatgat gatttagtaa tagctgaata
     6181 cagtgcaact gttgttggtc ttgaaatatt aagagcaata ggtgaagaat tggaagaaga
     6241 aatgagaaag aaagctgtag ttcaaatggc aataggcact ctgtcctact ccgagcttga
     6301 agcagttgaa catatttttg ctgaattgga tggaaaagaa ggtctacttg tagcaagtaa
     6361 gatagctgat agagttggta taactaggtc tgtaatagta aatgcactta gaaaatttga
     6421 gagtgcaggt gtgatagaat caagatcatt aggtatgaaa ggtactcata taagaatact
     6481 taatgacaaa cttacagatg aattaaaaaa attaaaaaac aatcaataat ctatatttta
     6541 taggtttaga ttagaaaagg tatctatttc aaatagatac cttttttaga cgaattatta
     6601 aggtaatgat aagtatatta aaaaggagtg agaaaatgcc tttagcagat aaaataagac
     6661 caaaaacaat gaatgatgta ataggtcaaa gtcatataat aggtaatgga aaaatattgt
     6721 ctaagatatt acaatctaat tttttaccaa atatgatatt ttttggtcct cctggtgttg
     6781 gtaaaactac agtagcggaa ataatagcag aaagaagcaa taaaagcttc tataaaataa
     6841 atgctactaa ttcatcttta gaagatataa aaaaggtgat atcagagctt ggaagtataa
     6901 ataatgtaaa tggagtccta ttatatatag atgaaataca aagttttaat aaaaaacagc
     6961 aacaatcaat attagaattt atggaaaatg ggagtataac tttgattgct agcactacag
     7021 aaaatccata tcattatgta tataaagctc ttttaagtag gtctactgta tttgaattta
     7081 aacctttaga aaaacttgat gtagaaaaag gactgaaaag agctgttgaa gtattaaatg
     7141 aagatagcta tatggatatt gagtgtaata ataatgctat tgaagatata gcaattcttt
     7201 ctgatggaga tatgagaaga gccttaaata ttttagaagt ggtagtctat tcaactaaac
     7261 caaataagga taatataact tatatagatt cagatgtaat aagggcatct acatttaata
     7321 aaattataaa ctatgacaaa aatggtgata gtcattatga tattttaagt gcatttcaaa
     7381 aatctataag aggaagtgac ccacaggcat ctatacacta tctagcaaga cttataaaag
     7441 gtggggattt gataagtata tgtaggagac ttttagtaat agctagtgaa gatataggtt
     7501 tagcttatcc aaatgctatt gtaattgtaa aggcatgtgt agactcagct atgcaacttg
     7561 gatttccaga agcgaaaata ccattagcag aagctactat tcttttggct acatctccaa
     7621 agtctaattc tgcatgtata gcaataatga aagctatgga tgaactggat aaagagtttg
     7681 ttaaggatat accaaatagt ataaaggatt ctcactattc tggttctaaa gaaataggta
     7741 ggggttgtgg atacaaatat cctcataatt ttaaaaatca ttatgtaaag caacagtatt
     7801 taccagatag cataaaagat agtatttatt acataccaca agaaaataaa actgagaaaa
     7861 atataaaaaa atatttagaa tatctaaata gtgattttta gactgaactt tatgtatcgg
     7921 gggtagatat tatgattata aaacttaact caacttatca cagtagagtt atgaaatact
     7981 taaaaaaaga acctgagtac aatttgttta taatagggga tattgaaaga tatggatatg
     8041 gaaataattt tttaaatata tgggcagatg tgggtgaaca tggtgaaata aaggcaatat
     8101 tacttaaata ttttgaattt atgatatttt attctgatgg agagtatgat gttgaaggtt
     8161 tttataatct acttcgtaat actaattacg aagaaatatc tgggaaaata tgtgctgtag
     8221 atgctttagc aaagagatta ggacttaata atttaaaggt agttgatttt tgcaaacttc
     8281 aaactaaaaa gtttttgata gataataatt gtaatgcaaa ggttaaaaga attaggcttg
     8341 gaaatctaaa aaagacagtg aaactttatg acttaataga tgaatttcac agtacaactt
     8401 tagagaattt aaaaaatgga ctaaggacag gtagaggata ttgtatagag attaacaaac
     8461 aggtggtgtc aatggcaaag agtacttcag aaaataggac tcatgcaatg ataattggag
     8521 taggaacaca tcctaaatat agagctaaag gacttgctac aaaatgccta attaaactat
     8581 gttcagagtt actgagagaa aacaaaattc cttgtttgtt ttatgataat gaagaggctg
     8641 gaaagatata taaaaaacta ggatttgaaa acattggaaa atggggaatt tattcaaaat
     8701 aaaaaattaa aattaatctt gacaaaatta gtagggtata ataaaattat cttgaaagta
     8761 attcctacta aaacagtatg aattagattt ctatatagag gtgataatat atgaagttgt
     8821 ctactaaagg tagatatgga ctaaaagcaa tgtttgaatt agcattaaat caagacaatg
     8881 gaccagtatc attaaaattt attgctaaga agcaaaaaat atcagatcag tacttagaac
     8941 aaatattttc atctttaaaa aaatcgggtc tagtaaaaag tgtgagaggt gctcaaggag
     9001 gttatttgct ttcaaagaat gctgaagaca taacagtagg agatatactt gtagtattag
     9061 aaggaccagt tgcattatct gactgtgtac tagatgaaga tgtatgtgaa aactcaaaca
     9121 tgtgtgtaac aaaaatagtt tgggaaaaga tgaaaaaggg tatagaagat gttatagatt
     9181 caattactct taaagatatg ataaatgact ataacaaaaa taaattagaa aatgatataa
     9241 caaatattaa aaaatagatt tggagatgaa tgtaatggaa aaaagaagat tgtatatgga
     9301 ttattctgca acaacaccta ttaaaaaaga agtattggat gcaatgatgc catacctaac
     9361 agattatttt ggtaatgcat ctagttttca cacattcgga agagaagcaa aagatgcttt
     9421 ggataaagca agagaacaag tagcagccct tataaatgct gaacctagtg aaatatattt
     9481 tacagcaggt ggttcagaaa gtgataactg gacactagag ggtgtagctt atgctaataa
     9541 aaacaaaggt aatcatataa taacttcaaa aatagagcac catgcaatac ttcatacatg
     9601 tgaatactta gcaaaacatc atggatttga aataacttat ttagatgttg atagcgaagg
     9661 aaaagtagac ttaaagcaac tagaagattc aataaaagat actactatac ttataagcat
     9721 aatgtttgca aataatgaaa taggaactat acagcctatt aaagaaatat ccgaaatagc
     9781 taaaaaacat aagatattat tccacactga tgcagtacaa gctacaggta atattccagt
     9841 agatgttaaa gaattaggaa tagatttgat gagtatgtct tcacataaaa tatatggacc
     9901 aaaaggtgta ggagctttat acataagaaa aggtgtaaga cttcataact ttgtccatgg
     9961 tggagcacaa gaaaaaagca aaagagctgg tacagaaaat atacctgcaa tagttggata
    10021 tggaaaagca gcagaattag ctaaggaaaa tatgcaaaat catgtagaaa ctttaactcg
    10081 tcttagaaat aagttaatag atggtgtgtt agaaagaata ccttacacaa gagtaaatgg
    10141 tagcttagat aatagattgc caggaaatgc aaactttgca ttccaattta tagaaggtga
    10201 aggaatactt ttattattag atatgttagg aattgctggt tcaagtggtt ctgcatgtac
    10261 ttctggttca ttagaccctt cacatgtact tcttgcaata ggtttaccac atgaaatagc
    10321 acatggttca ttaagactta cagttggaga ttttacaaca gatgatgata tagattatat
    10381 actagagaac ttaccaaaag taatagaaag acttagaagc atgtcaccac tttatgatga
    10441 tgcgaagaaa caaggtttag taaaatagac ataaagataa agcataaaaa atagacataa
    10501 attgagtagg aggaaaagat aatgcaatat agtgataaag ttatggaaca ttttatgaat
    10561 ccaagaaata tgggagaaat tgataatgca agtggtgtag gtgaagttgg aaaccctaca
    10621 tgtggagata taatgaagat atttttagac atagatggag atgtaataaa ggatgttaag
    10681 tttaaaacat ttggctgtgg ttcagctata gcaagttctt ctatggcaac tgaaatgata
    10741 aaaggaaaaa ctataaaaga tgcattagaa cttacaaata aagctgttgc agaagctttg
    10801 gatggtttac caccagtaaa gatgcattgt tcagttttag ctgaacaagc tgtaaaagca
    10861 gcccttatag attatgctca aaagaacaat atccatatac cagaattgga tggatatgtt
    10921 atagatgatg ctcatgacca tgatgttgaa gaagaagaat aaaaataagt gattgtgtta
    10981 tgagaagttt acaatgatta aaaagaagta ctaatgatat aattaggaca taaaaaatag
    11041 ctgtttttaa aatggctatt ttttattgcc taaattaagt attttaactt gatttagagg
    11101 ttaattagtt aacaaaataa tccataagat tcaaataata aagatgatta tttttttaga
    11161 aaatagaaca agctatattt taaataagta caatacttgc tctatatggt ataataataa
    11221 gtaatgcaaa agatgaggtg aaatttgttt atgaataaaa aagtaatgat aggtatgagt
    11281 ggaggagttg atagctctgt agctgcatat cttttaaaac aacaaggata tgatgtcatt
    11341 ggtgttacta tgaagttgtg gcaagatgat gatgttgttg aaattgaagg tggttgttgc
    11401 tctttatctg ctgttgaaga tgctagaagg gtagcaaata aaataggtat accattttat
    11461 gttttaaatt ttagagaagt gtttaaagaa aaagtaatag attattttat agatgaatat
    11521 ttggaaggaa aaactcctaa tccctgtata gcttgtaata aacatataaa atttgatgat
    11581 ttttataaga aggctagaca aataggttgt gattatgtag ctacaggtca ctatgcaaag
    11641 atagaaaagg atgaaagtac aggaagatat ttgctaaaaa aatctgttac agataaaaaa
    11701 gaccaaactt atgcacttta taatcttaca caagagcaac tagagcatac tttattgcca
    11761 ataggtgact atgaaaaaga tagagtaaga gaaattgcta aggaaatggg tatggcagta
    11821 cacaataagc ctgatagtca agaaatttgt tttgttaaag ataatgacta tgcaaattat
    11881 gtaaaaaaac attctaaaaa gcgtattgaa gaaggttttt ttgtagatac taaaggcaat
    11941 atacttggaa aacataaggg tatattatat tatactatag gccaaagaaa agggcttgga
    12001 attacttttg gaaaaccaat gtttgtaata gatataaatc caataaataa taccatagtt
    12061 cttggagaca atgaagattt atttaaaaag gaacttattg ctaaagatgt taactttata
    12121 tcaatagata cgttagaaga acccttaaga gttcaagcca aaataagata ttcagcaaaa
    12181 ccatcaccag ctactattca tagagtagga gaagatacta ttaaaatagt ttttgatgaa
    12241 gctcaaagag ctattactaa aggtcaatct gtagtaatgt atgatggaga tattgtagta
    12301 ggtggaggta taatcgagaa aagtttataa aaacatctaa aaaattaaaa aaataagcaa
    12361 taaagtatat taagagtaaa taaagtttat aaaataaatt tattgtatat gaaaatatag
    12421 tattgattaa ttttgtaaaa tttagtaata taattagtat attaattaaa aaacaaatac
    12481 agtgataagg aatagtaaaa tatttgatgt cttacagaga gagggaaatg ctggaagccc
    12541 ttagaatatg atatttgaag cagcctttta gtagcaatct tgaacaattt atcttttata
    12601 aagaagattt aagtagagat tgacggtagt tacgttatag ctaaggtttt ccgaagatat
    12661 catactttgt atgataatta gggtggtacc gcgattgtcc tcgcccctat aaatttattt
    12721 atagggacga ggttttttat ttaaacgaaa aaataaaatt tattaggagg aacatgatat
    12781 ggaaaagatg ggattaaatg aaataagaag taagttttta gaattttttg agtcaaaagg
    12841 tcattatgta gcaaatagtt attctttagt accaaacaat gataagagtt tgttacttat
    12901 aaactcaggt atggcacctt tgaaaaacta cttttcaggt gtagaagtac ctccaagtgt
    12961 gagaatgtgt acatctcaaa aatgtataag aacaggagat atagaaaatg taggtataac
    13021 tgcaagacat gctacattct ttgaaatgat gggtaatttc tcatttggag attactttaa
    13081 aagagagtca atcaaatggg gatgggaatt tgttacagaa tggctaaata taccagaaga
    13141 taagatatgg gtgactgtat atgaagaaga tgatgattct tatgatatat gggcaaaaga
    13201 aatgaatttc ccagaagaaa gaatggttag acttggaaaa gatgataatt tctgggaaat
    13261 aggtacaggt ccttgtggtc cttgttcaga aatatatttt gatagaggag aagaatatgg
    13321 atgtgacaat ccagactgta aaccaggctg tgattgtgat aggtatttag aattttggaa
    13381 ccatgtattt actcaatttg atagagatga agaaggaaat tatagtctat tagaaaacaa
    13441 gaatatagat acaggtatgg gtcttgaaag aatgggatgt attatgcaag gcgtagacac
    13501 tatctttgaa gttgatacaa ttaaatctat actagaagca gttgagaagt taacaggagt
    13561 aaaatatgga gagaatccaa aaaatgatat atcaataaga ataataactg accacataag
    13621 agctgtaaca ttcttagtta gtgatggagt gcttccatca aatgaaggaa gaggatatgt
    13681 tcttagaaga ctccttagac gtgctgctcg tcatggtaag ttattaggag taaaagaatt
    13741 atttttacag aaactaatag atgaggttat aaaagtaaat gataaagcat atccagtttt
    13801 agttgaaaaa gaaagttata ttaaaaaagt agttggaata gaagaagaaa aattcaatga
    13861 aactatagac caaggaacag aaatattgaa ctcttatata gaagtattaa aaaatgaagg
    13921 aaagactgtc ctaagtggtc aagaagcttt taaactatat gatacttatg gcttccctat
    13981 agacttaact aaagaaatat tagaagaaga acatctatct gtagatgaag aagcttttaa
    14041 tgaagaaatg gaaaagcaaa aagaaagagc tagaaatgct agaggaaata tggatggaga
    14101 aagttggaag gaagaccctc tatcaaaatt agaatctaca gttgacagta catttaatgg
    14161 gtatagtgaa atttatggtg aaggaactat tgaagctata gttaaagatg atgagctagt
    14221 tcaaagtgca gaagaaggcg ataaggtatc tatagtacta gataacacta ctttctatcc
    14281 tgaaggtgga ggacaagttg gagattgtgg attgataact aatgaaaact tagttttaga
    14341 agtattaaat actaaaaaag gtgctaataa tagtataaaa catataggta taataaaatc
    14401 aggaagaata agtaatgggg acaaagtaaa gacactcgta gatagagaaa ctagaatgtc
    14461 agctgcaaga aatcacagtg ctacacattt acttcataag gcattaagag aagtattagg
    14521 agaacatgtt aatcaagctg gttctttagt aactccagaa agacttagat ttgatattac
    14581 tcactttgaa gcaatatcaa atgaagaatt aaaagtaata gaagaaaaag taaataatgt
    14641 tattttatca tctttagaca taaaatgtga tattatgaat ataaaggaag ctaaagaaaa
    14701 aggagctaca gcactatttg gtgaaaaata tggtgatgag gtaagagtag tttctatggg
    14761 agattattct acagaacttt gtggaggaac tcacttgaca aatacttctc aagtaggaat
    14821 gttcaaaata ttatctgaag gtggagtagc agcaggtgta agaagaatag aagcaataac
    14881 aggaaaagct gtttatgaat acctaaaaga aagagatgga attatctctg aagtttgtgt
    14941 aaacttaaaa tctaaagaag ataatttaat tcaaagaata agttctttat tagaagaaaa
    15001 taaaaactta tctaaagaat tgcatgatat gaaagctaag atgagtttac aatcagttga
    15061 ttctattttt gattctaaag ttgaagtaaa tggagttaat ttgataacta ataaatttga
    15121 aggtatggat atggatacac taagagaaac agcagataat ctaagagata agcttggttc
    15181 tggtgtagta gtacttgcaa atgtagtaga tgataaagtt aactttgtag ttactgccac
    15241 taaagatgta ttagataagg gaatacattc aggtaatatt gttagagaag ttgcaaaaat
    15301 agctggagga aaaggtggag gaagacctaa tatggcacaa gcaggagcta gtgatgtatc
    15361 taaggttgac caagctttaa gctatgcaag tgaggttatt aagacacaag ttaaataaca
    15421 ttcactcaag gaggttttta gtttggaaaa agatttagac tacactatga aatttgaagg
    15481 aataccagaa gataggatga gtgtaggaga tactattgat tttgtatata aagcattagt
    15541 tgaaaaagga tataacccta taaatcaaat tatagggtat ctcctttctg gtgattctag
    15601 ctatataact agtcataaaa atgcaagagc tataattaaa aaatttgaaa gagatgagat
    15661 acttgaagaa gtaatcacgc actatctgaa tagaaagtag gtcaaataaa tgagaaaaaa
    15721 gattatgtcg ttgtttgcaa tagtcattat gatttttgta tcaactgcta gctatggttt
    15781 tgctgaagaa aaaggcaagg tcatatttat tgatatgaat aggaccaatc ttggaagtat
    15841 gatgaatatt ccaatcttga aaaatgagat agaaaagaga ggctatgtag ctctcatgaa
    15901 tataagaggg gatcaaggta ctgatgatag aaggtcttgt gcaagtatgg gtgcaggaac
    15961 aagagctagt gtaactactg aagactatat caattttcaa aaagtagacc aagatactgc
    16021 taaagctttt gaagtcgcaa caggtcaaaa agctaaagaa ataaatgatt taggcattaa
    16081 tagatctata aatgaaaatc ttgagtatgg agaatatgga gcaaatttag ggtcattagg
    16141 aaaaacttta tcagaaaatg gtttaaagac atctgtaatt ggaaatgctg atataattga
    16201 aaataatcaa ttagttaaaa acagaaactt atgtttagta gctatggacc aatatggaag
    16261 gattgattca ggtaatattg ataatattaa taagaaagat tctaaaatgc catttggaat
    16321 atctacagat tatgataaat tactagatga aactaaaaaa tcctataaaa atagtgatgc
    16381 tatatttgta gaacttggag atacatatag attagatatg tatagattaa atctaaatga
    16441 taatacttat gatacaatga agaaaaatat tgtaaaaaat ataaataaat atcttgaaca
    16501 agtattttct atggttggag aaaatgacac tgtttatata gcaagtgcat ttccaagtga
    16561 cctagattat aagaataaga gaagactttc tccaataata aaatttagtg gagaagggaa
    16621 aggtctactg gaatctgcca caacaagaaa ggctggaatt gttgccaatt tagatgttgg
    16681 agtagatata ctaggtacat ttggtcttga aaataaagag atggtaggta gaagttatac
    16741 tttagtaaat aaagaggaca atattagcta tctaaaatca ctttatgaaa ggatagtgtc
    16801 tatatcttca ataaggtcaa ctattataaa tggctttgta ggcgtaatat ctgcttcttg
    16861 ggtaattgcc atgtttgcga ttttctttag aaattatatg ccacataaag agcgtgtatt
    16921 caaagtactt aaggagttta taaaattagg cataataatg ccactatcat ttatgttagc
    16981 tcctttgatg aattttaaaa ctccaatttc aatatctttg ggaatatttg caactacgct
    17041 aatattatac ttgttaggta gaaaattatt taaggacaat gatataaagc acatgggatt
    17101 ttttgcaaca cttactattt tagtcatagt tatagattct gtatttggaa catacttaat
    17161 gcaaaataac ataatgagtt atgatgctgt cataggagct aggtactatg gtgtaggaaa
    17221 tgaatatgaa ggtgtaacta tagcaagtgc agtatttggc ttagcagtat tattacatta
    17281 taagaaggta tctaaattac ttgttgtaat tttttcatta ataatactta taactagtgc
    17341 atatccttca atgggtgcaa atgttggtgg agctatatca gaatgtgtag catatttgtt
    17401 gtttataatg cttatatatg acattaaaat agattttaag aaagctgtat tattagctgt
    17461 ttcagctgta gttgtcgttt ttgcatttgc agctttagat atactttcag gtagtgaatc
    17521 tcatttaggt atgtttgtac agagaatttt cctaaatgga ccaggagaga tattacaaac
    17581 atttggaaga aaaattcaaa tgaatatgca actagcacag acaagtgtat gggtaaatat
    17641 tttacttgta ggaattggag taattgcagt gcttatattt aaaccatcta gacactttag
    17701 aaagataatg aataattttc caatcttgtt taaagggttt attgcatcta tggttggttg
    17761 tataattact cttttagtta atgattctgg aatagttgct gcatctacag catctatata
    17821 tatacttata ccactaataa taataagtat aaatatgata atatttgaaa aaaataacta
    17881 aaaaaatttt tactgttcat ataggattta aataaaaaat gcaatatgat tattaatgta
    17941 aactggatgc ttacataaaa aatcatattg caatttttat gattgataat gtaaaattct
    18001 atgaagcagt aagcctgtga ccttagttat gagaggttca gaatatgtat ttaatctata
    18061 gaatataaat agataaagga ggaactatgt tagatggaag aataatgggt cttgatgttg
    18121 gtgataaaac tattggagtt gctgtgagtg atttgatggg attaactgca caaggtgtaa
    18181 aaacaataaa aagagttggc aagaaaaaag atattgaaga gctaaaagca ataataaaag
    18241 aaaaacaagt aaataaaatt gtatcaggat taccaaaaaa tatgaatgga actttaggtc
    18301 cacaaggaga aaaagtaatc aagttttgtg aattggtaaa agcagaaaca ggtattgatg
    18361 tagagttttg ggatgagagg ctttctacag tagcggcaga gaggtcttta ttagaagcag
    18421 atgttagtag acaaaaaaga aaaaaggtta tagatatgtt ggcagcagta ataattttgc
    18481 aaggatattt agattttaaa ataaattcat aaaagaacta taaaattgaa tacttatgat
    18541 atataatata aataataaaa ataaaaataa atttgaggtg aatgacatta tggaagaaaa
    18601 tataataaat ttaatagacg aaaatggcgt tgaaagccaa tttgaaataa tattaacatt
    18661 agaagcagaa ggaaaagagt atgcaatatt aatgccttta gatgatgaag aagctgaaga
    18721 agctttaatc tttagaattg atgaggatga agaaggtgaa atcttaatac ctttggaaag
    18781 tgatgaagag tatgaaactg ttgtagctgt gtatactgct ataatggaag aagaaggttt
    18841 aaattacgac gaagatgaat caaatggact taactagata agtaattaag tttgtatata
    18901 aaaatcagat atttattata tttgctttaa ttaaacctta ttaattgtaa aagcatttat
    18961 agtatatatc tgtttttttg ttttttatta aaaataccaa aaaatttatg aattttggtg
    19021 aataatatat cttttatggg tatatattta tattatgaga ataaattaaa tttatttaaa
    19081 tgtcttgaca aaaaatgaat tatcaattac aatattattt aataaagatt atcaattgaa
    19141 aatagtaagg gtgatgttat ggcaaataca atggatttat taaaagacaa gttaaaagaa
    19201 actggtttta agattacgcc acaaagaaga gcaatagttg aaatattgct taaacatgac
    19261 cattcacatc taagtagtga ggaaatctat gatttagtta gggttgactg tcctgaaata
    19321 ggcttagcta ctgtatatag aacaatgcag ctattggatg aaataggatt aatatcaaaa
    19381 ctaaacttag acgatggatg tataagatat gagattagtc tacataaaga agactgtcat
    19441 aatcatcatc atcttatttg taaaaattgt ggaaagataa tggaagctaa agaagacctt
    19501 ttagacaata tagaaaaaga gatacaatca ttgtacaaat ttaaaatact tgatcatgat
    19561 gttaaatttt atggactatg tgacgagtgt aatggagtaa gcgattcaga agaataaact
    19621 tctattgctt aatgtcgatg gaagaatagt atagaacttt tatattctat gctattcttc
    19681 cattttttat tataaaccat ctataaaatg caaatataca gttttcaaag tgattatagt
    19741 tttaatattg aaaatataat taatcctata agtacaaaaa tgaatgattt ttctatgaaa
    19801 ttactaaatg gtcctccaac agttaaaaac cctaaagaaa ttttcttttc gcttagggga
    19861 taaaaaattg gtattccttg ctttgtaaat aaatctccta aaaacatatg gcttgcataa
    19921 ccaatcgtac cataaaaaga taaattatta atattagtga ttaattctat ctgttttagt
    19981 aaaaaatata ctattatagt tgcaaatatg ctatgagaaa aacttctgtg ctttaaccag
    20041 gggaaaattg acaacattag acaaaaaatt gtaaaatata tttcaacttc tatgatgtat
    20101 aagcatatag ctaatagtat aaatattaat gatagaaaaa cttttcttag aaatgtatgg
    20161 gttagttttg cttcaataat tataattgca acaaatgtaa ttattgcgct taaataaaaa
    20221 ttatcattta tatttattga aataaagaaa ataataatat taacagcata tataaaaatt
    20281 ctatatatgt atttggatac attgtttttt agaatgaaat ttgagaccat agaattagat
    20341 ttatccaaat cgggtagtac agcaaaagta gctgaaacta aaatatcaac taaagatata
    20401 ggaattttaa atattaaaga tgtctggata gctgttaaga ttcctattgc acaatgagtt
    20461 ttaccacgca taaatcctcc taaattatcc gatattaaat catataatat ttagaggata
    20521 tcaaaataaa ataggtttgt caaaataata cgttaaatgt attttaaata tagcttaaaa
    20581 tatgatacga attatggtac aataagaata actggtagcg agtgcgtgat ttgatattat
    20641 ttatgattgt agaacgtgtt aaaaaattaa atagacagaa ggagatgatg caattgttca
    20701 aaaagaacac caataagata aaggtaatgg ccttgggtgg acttaacgaa gttggaaaaa
    20761 atatgactgt tgttgagtat aaagatgaaa taattgttat agatgctgga cttagttttc
    20821 cagaggatga aatgttagga gtagatatag ttataccaga tataacttat ttagtaaaaa
    20881 atagggataa aataaagggt atatttatta cacatggaca tgaagaccat ataggagctc
    20941 ttccatatat attaaagaaa ataaatgtac ctgtatatgg ggcaagactt agtataggtc
    21001 ttatacaagt aaaacttaaa gaacataaga tgaataatgt taagttaaat gttataggtc
    21061 ctagacaagt tataaagctt gataatatgg aagtagagtt tttgaaaaat aaccatagta
    21121 taccagatgc ttactctatt gcaatacaca cagaccaagg tataatatac cacacaggag
    21181 attttaagat tgacttgact cctattgatg gagatgttat ggatatgcat agaatctgtg
    21241 agttgagcaa aaaaggagtt cttttaatgc tggctgatag tactaatgca gaaaaaccag
    21301 gatttactat gtctgaaaag actgttgggg taggtcttga cgaattattt gcaaaaggaa
    21361 atggcagaag aattatagtt gctacctttg catccaacat acacagatta caacaaataa
    21421 taaatactgc tgagaagttt aatagaaaag ttgctatatc aggtcgctca atggtcaatg
    21481 tagttggagt tgcaaaagaa ttaggatatc tagatatttc tgatgacatg cttatagatt
    21541 taaatgatat atgtaagtat gaagatagtg aattagtgat aataacaact ggttcacaag
    21601 gtgaacctat gtcagctctt gctagaatgg ctttttcaga gcacaaaaaa gtagaaataa
    21661 agagtggcga tttagttata atatctgctc atccaatacc aggaaatgaa aaattaatat
    21721 caagagtaat caatttctta tttgaaaaag gagcagaagt agtatacagt gatatagctg
    21781 atatacacgt ttctggacat gcctgtcaag aagaattaaa acttatacat gcattagtta
    21841 gaccaaagtt ttttatgcca gctcatggtg aatatagaat gttaaaaaga catgctgaaa
    21901 tagctgaaca gcttggtatg gataaagaaa atatttttgt catgcaaaca ggagatgttt
    21961 tagaattaga taaaaactct gctaaggtag caaatcgtat acaaacagga aacatattag
    22021 ttgatggtct aggagtaggc gatgtaggta atatagtttt aagagataga aagcatttat
    22081 cagaagatgg tcttatgata gttgtagtta ctatttctaa ggatgagggt aaagttttag
    22141 caggtccaga tataatttcc agaggattcg tatatgtaag agaatcagaa gatttaatgg
    22201 atggagcaaa ggatataatt aagaatgttc taaatgaatg tgaagagaaa aatattaaag
    22261 aatgggctta tttaaagaat aatattaaag aaaacctaaa agaatattta tatcaaaaaa
    22321 ctaaacgaaa tccaatgata cttcctatca taatggaagt ataatataaa aaaactataa
    22381 atttgaaaat tagacctctt gtaattttta aagtaatatc aaaaatccaa ttgatattat
    22441 cttaagaatt acaagaggtc tttttaatac ttgatttaaa aaaatatata tattacataa
    22501 aataccaatt ataacaatct tttattagta aaaactactt atatataaat tttactgata
    22561 ggaggattct tatggatgat gtttcaagac aaaatgctat aaaagcttta aaacaaacaa
    22621 aaatggaaat agcaggagag tatggaatga attatgaaga tgcttttgaa ataatagaaa
    22681 atgcgtctaa taaaggtgtt ttagaaggtt actttaaaaa gcttgaaaag aaaaaaaatt
    22741 taggtcaagg tatatctagg catttagaat aaaaaaagtt aaatgtgaga aaataagttt
    22801 aaataaactt taagaggtga catcgatgaa aaaattagct agtactgctt tggctatatt
    22861 aattgcgctt actcccttaa gcttttcttt tgcaaacaat aaggaaaatg ctgatgccaa
    22921 ccaactaaat atatcttcaa aatcagcaat tttgatggat gttggtagtg gacaaatttt
    22981 atatgaaaag gatgctcata aaaagcttcc tcctgctagt gtaactaagg taatgactat
    23041 gctacttata gtagaagctt tagattcagg caagattaaa cttgatgatg aagttcaagt
    23101 aagtgaaact gcatctagta tgggtggtag tcaaatattc ctagagccag gagaaactca
    23161 aaaggttgat acattgttaa aaggtatagc tgttgcttct gcaaatgatg cttgtgttgc
    23221 tatggctgaa cacttagctg gaagtgttga agggtttgtt gatagaatga atgctaaagc
    23281 aaaagagtta aatatgaatg atactcattt tgcaaataca aatggacttc cagttgcaaa
    23341 ccactataca tctgctcatg atatagcttt aatgtctaga gaactattaa aacacgaaat
    23401 gataagtaaa tacttaacta catggatgga taaggttgta gtagggaaga aacaagttac
    23461 agtaggtctt gcaaatacta acaaacttat aaagcattat caaggagcaa ctggtgttaa
    23521 aactggcttt actcaagaag cgaaatactg tttatcagca tctgcaaaga gaggtaatac
    23581 tcatctagtt gctgcaactt taggagcaga gacttctcca gaaagattta atgatgcctc
    23641 tagcctttta acctatggat ttgctaatta tgaaagtgtt aagttatgta gtaaaggtga
    23701 taatatagca acacttacat tggataaggc tgatgaaaac aaagttaaat tggttgctaa
    23761 agaagattta aatgctctta ttaaaaaagg aagttctaaa gaatttgaga agaaaattga
    23821 aatagtaaaa aatcctaaga tgccaattaa aaaaggaact gttttaggaa agataaagat
    23881 atgtaaagat aaaaaagtaa taggcgaagt ggaattaata aatactaaag atataaataa
    23941 ggcaagctat ctacaaatgc ttcaaagaat aattgataat atgatataaa taattatcag
    24001 atgaaatata attataatac acaaagatat gattataata cacataaaaa atacccttat
    24061 gtaattattt gccatagggg tatttttatt tactatatag aagtatttta taatcttatg
    24121 atttttatag aaatatagaa aagtttatgt atcttaaaat atcatttgat ttaatatcat
    24181 atatagggtt gtttaacaat tatctaagta aaggcatact accagtaagt ttattcactt
    24241 tctttttatt cccataaatt gctatcccaa aatactgtaa ctcatcttct ggaacactag
    24301 caatcttttg tgtaaattct tcataaagat tgcaactttg tgctacatct gaaaaatcta
    24361 caacagttaa atcgttaaat tccaaagtat ataatttttt acgtaaatct tttattattt
    24421 ctttgtttcc ttttaaaata ggtacaggaa tgtcaataat tcctaagtgg ctattacctg
    24481 ttttgtcaat tacatctggc ccaacagttt ctggggcatg ttttcctagt gtaattccca
    24541 ttatagctgc tgtattggat ataattccca ttggtaaatt ttcgtctatt accatgacac
    24601 attttgaatt tttattttcc atttttattt ctccttttta ccttatttaa agttgtttga
    24661 atcatcattt ataaatatat tttgatactg ctttggtgta agaccaataa aattcttaaa
    24721 aaagtttgta aagtgacttt ggtctacaaa tcctgtttgt atcgcagcat ctataggttc
    24781 tacgcctttt tctaaaagct ttttagcttt gtttacccgt atattttcta ggtatctata
    24841 tggtgtaata ccttttaatt tcgtaaaatt acgaagtaga gagtacttat tcatacctgc
    24901 tatagtgctt aattcgtcaa gtattatatg ttcagcataa ttattttcta gataatcaca
    24961 tactgcttga atttctatat ttgtattttc caagttagat tgcaaatttg gctctgtatg
    25021 ttcttcaatt aattgttcaa ttaggaagaa aaaaagctct tcctttttaa aatccaattc
    25081 ttcttccatg attatatagt gtaattcttg tagtagaggt actaactcac tgcgaaatat
    25141 aacaggttga ttaaattttg gtaaataatt tttacctgta atttcaaaga cggttttttt
    25201 cattatttct ggcttaatat taatacatct gtaatcaagt actttatcat ctatttgttc
    25261 acaagtatga ctatcaaaag ggttaaaaag tagcaaatct ccagtacttg tggtatactc
    25321 tttattttta caagataaat atctttgacc tttttctata aaaccaatta cataatgttc
    25381 atgaaaatgg tttgggaatt tttgcattat gcctttaaat ctatatgctt caatttttaa
    25441 ttccctatca aagcatactg tgcgttcttc ttgtctcact taggtaacct cctttcatgt
    25501 tatacaaagt gtaacacata tttttttata cttcttgtat aatattgact aagagacaat
    25561 aatgtcaatt catgtcaata taatattaca cttacagtag gtgtaattat tatattttca
    25621 caatgaatat gatataataa taaaagttat aaaataaaaa gcaaatttta attttatgcc
    25681 tagaagttaa aattattata atgtaaaata attttgatta taagctattt aattgttata
    25741 ataatattaa aaatttaatt taggagatgt acattaatgt taagtatgaa tataggagaa
    25801 atagtagcaa gtttagttgg tatagctatg gcaatatcaa tacatgagtt tggccatgct
    25861 tattcagctc acttattggg agatgataca gctaaagctt atggtagaat gacattaaat
    25921 cctgtaagac atgttgaccc aataggtctt ttggctatgt ttatccttaa gatagggtgg
    25981 gcaaagccag taccagtaaa tccaaataac tttaaaaact ataagatagg aaacctgata
    26041 gtttcacttg caggcgtttt ttgtaatgtt ttgactgcta tactgtgtgt aattatcaat
    26101 aagtatgtgc atatgtatgc tataaataca atagctgaat atgtgttttt atatagtatt
    26161 ggatttgcag cttttaactt acttccgata cctccacttg atggatgggg agttatatct
    26221 acatttgtac catataaatg gaatgaatat ttgtataaat ttgaaagtat gagttatata
    26281 attttattaa ttgctttatt tactggtatg tatagaataa tattaacacc aatatatgca
    26341 gtgttgctaa aaatagtata tttatttgtt taatataata aacagattaa agagggattg
    26401 atatgaagta taatatacaa ttacaagtct atgagggacc attagattta ctttatgatt
    26461 taataacaaa gcataaaata gatataaagg atatatccat aatagatata actaagcagt
    26521 atctaaatta tttgaaaatg ctagataaaa tggatttaga gataactagt gaatttataa
    26581 ctatggcatc aaaactttta gagataaagt ctaaatatct gctttataag caaaaagatg
    26641 aagaagaaga cccaagaata gaacttatgg aaaagttaga agaatataga aagtttaagg
    26701 tagcatcaca agatataaaa gaaaatataa cttatgttaa tgagagattt tatagaaata
    26761 aagaagagat aattattgat gacaatgtgg atttagaaga tatatcaatt gaagcaataa
    26821 aaaatatact tccatacata tttaaagtta aaacatcaca gatagaaaat gctaatgatg
    26881 agaaactaga taaaatagta aggaagaaaa taatatctgt agaagaaaaa atattatata
    26941 taagagatat tataaaagag aagattgaag ttacttttac aaatattata aaatcatatg
    27001 aaaatgatga aataatagct acttttttat ctatattaga gcttataaaa gaaaaagaaa
    27061 tagtcgtagt acaagatata ttttttgatg atattctaat aagaaaaagt tcggagtgtt
    27121 aaaatgaaaa gagaagatat aaagtatatt atagaatctg ttatgtttgc atatggtgag
    27181 ccaataagta taaaagaatt aaactatata ataaataaag aactttcatc aaaagaaata
    27241 gagattatgt taaatttact tattgaggag tacagagaac aaaatagagg tattcaaata
    27301 atcaagttag agaataagta ccaaatgtgt acaaataaag actatgcaga atatataaag
    27361 aagattattg aaccaaagaa aaagaaaagt ttgagccaag ctacattaga aactcttact
    27421 ataattgcct acaaacaacc aataacaaag gtagaaatag aggatataag aggagtaaaa
    27481 tgtgataagg tattacaaac tttgtttgaa aatgaactta taagagaagc tggtagattg
    27541 aataagatag ggaaaccaat aatttataaa acaacagatg aatttttaaa gttgttgaat
    27601 atagaaagtc ttgaagaatt gccacctata gaaaattatc aagaagtggc aacaaatgaa
    27661 taaaaatata aaatcacaat aatttagtat gtaattttaa attttaaaag atttaaaatg
    27721 ctctaagttg ttgtgttttt ttgtataaat ataaaattat
//
LOCUS       DAMJVI010000042        27345 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.044, whole genome shotgun sequence.
ACCESSION   DAMJVI010000042 DAMJVI010000000
VERSION     DAMJVI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27345
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.044"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            257..790
                     /gene="pyrR"
                     /locus_tag="PC545_001916"
     CDS             257..790
                     /gene="pyrR"
                     /locus_tag="PC545_001916"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2456684.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            1074..2285
                     /locus_tag="PC545_001917"
     CDS             1074..2285
                     /locus_tag="PC545_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2456685.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            2584..4236
                     /locus_tag="PC545_001918"
     CDS             2584..4236
                     /locus_tag="PC545_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2456686.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(4451..6226)
                     /locus_tag="PC545_001919"
     CDS             complement(4451..6226)
                     /locus_tag="PC545_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2456687.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            6522..7556
                     /gene="ltaE"
                     /locus_tag="PC545_001920"
     CDS             6522..7556
                     /gene="ltaE"
                     /locus_tag="PC545_001920"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2456688.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            7843..8673
                     /locus_tag="PC545_001921"
     CDS             7843..8673
                     /locus_tag="PC545_001921"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2456689.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            8677..9558
                     /locus_tag="PC545_001922"
     CDS             8677..9558
                     /locus_tag="PC545_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2456690.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            9588..10205
                     /gene="gmk"
                     /locus_tag="PC545_001923"
     CDS             9588..10205
                     /gene="gmk"
                     /locus_tag="PC545_001923"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2456691.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            10208..10474
                     /locus_tag="PC545_001924"
     CDS             10208..10474
                     /locus_tag="PC545_001924"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2456692.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            10479..11678
                     /gene="coaBC"
                     /locus_tag="PC545_001925"
     CDS             10479..11678
                     /gene="coaBC"
                     /locus_tag="PC545_001925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2456693.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            11917..14442
                     /gene="priA"
                     /locus_tag="PC545_001926"
     CDS             11917..14442
                     /gene="priA"
                     /locus_tag="PC545_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2456694.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            14460..14900
                     /gene="def"
                     /locus_tag="PC545_001927"
     CDS             14460..14900
                     /gene="def"
                     /locus_tag="PC545_001927"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2456695.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            14915..15844
                     /locus_tag="PC545_001928"
     CDS             14915..15844
                     /locus_tag="PC545_001928"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2456696.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            15854..16606
                     /locus_tag="PC545_001929"
     CDS             15854..16606
                     /locus_tag="PC545_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2456697.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            16675..17376
                     /locus_tag="PC545_001930"
     CDS             16675..17376
                     /locus_tag="PC545_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2456698.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            17438..18763
                     /gene="rsmB"
                     /locus_tag="PC545_001931"
     CDS             17438..18763
                     /gene="rsmB"
                     /locus_tag="PC545_001931"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2456699.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            18771..19802
                     /gene="rlmN"
                     /locus_tag="PC545_001932"
     CDS             18771..19802
                     /gene="rlmN"
                     /locus_tag="PC545_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2456700.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            19822..20574
                     /locus_tag="PC545_001933"
     CDS             19822..20574
                     /locus_tag="PC545_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2456701.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            20591..22606
                     /gene="pknB"
                     /locus_tag="PC545_001934"
     CDS             20591..22606
                     /gene="pknB"
                     /locus_tag="PC545_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2456702.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGAGANIGKTE"
     gene            22714..23451
                     /locus_tag="PC545_001935"
     CDS             22714..23451
                     /locus_tag="PC545_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2456703.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            23549..24433
                     /gene="rsgA"
                     /locus_tag="PC545_001936"
     CDS             23549..24433
                     /gene="rsgA"
                     /locus_tag="PC545_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2456704.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            24438..25109
                     /locus_tag="PC545_001937"
     CDS             24438..25109
                     /locus_tag="PC545_001937"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2456705.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            25118..25759
                     /locus_tag="PC545_001938"
     CDS             25118..25759
                     /locus_tag="PC545_001938"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2456706.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            25890..26519
                     /locus_tag="PC545_001939"
     CDS             25890..26519
                     /locus_tag="PC545_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2456707.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            26574..27230
                     /locus_tag="PC545_001940"
     CDS             26574..27230
                     /locus_tag="PC545_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2456708.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
BASE COUNT        10889 a         2766 c         5095 g         8595 t
ORIGIN      
        1 agcttgaaaa atttgaaata taaatatata atttgatttg atagattaaa aaattatctt
       61 taaacgatac agagatatcg taagacaatt gctaaattgc actgtatatt ttttatataa
      121 gagcttcttg tcttgtgatg agaagttttt ttgttgttta aattgaaatt ttaaataaaa
      181 ataaatttat aaaaaattaa aagaaaggag tagatttgtc aaatagatat attatatctt
      241 tgacaataat tatgatatgg tagaaaaagc tcaattaatg gatgaaaagg caatagcaag
      301 agcaattact agaataagtc atgaaataat tgaaagaaat aaaggtgtag aaaatctagt
      361 tttagttgga ataaagacta gaggtgtgcc aattgcaaat agaatttcta agaaaataga
      421 acaaatagaa ggaacaaaag tagatacagg agatattgat ataactttgt atagagatga
      481 tttggaaaaa attcatgttg aacctgtagt taaaggaact tatttagatt ttaatgtaaa
      541 tgataagaca gtagttttag ttgatgatgt tttatacact ggaagaactg tgagagcttc
      601 actagatgct attatagata taggaagacc taaatcaata caattagcag tgctagtgga
      661 cagagggcat agagagttgc ctataagagc agattatgta ggaaaaaatg tacctacttc
      721 tagacatgaa attatatctg tcagtttatt agaaattgat ggagaagatt cagttacaat
      781 caaagaataa ttatttttaa gtattatata aaagcttagc attgaatagt cagggaggag
      841 gataaagctt acatagattg cacaaaataa atattataat tttatggatg tgagtcttaa
      901 tgtagttttt attctccttt atttgagttt ttgatgtcgt aaaatgtaaa ataatgataa
      961 attatataat aagtatatag aggttaaaaa atagaatcta gggaatgata attaattaaa
     1021 agatattaat taaacagagg atttatttat atataataat tttggaggtt tttatgaaaa
     1081 aaacgataat gtctttacaa catctattag ccatgtttgg agcgacagta ttagttccca
     1141 ttttgacagg ttttaatccg tcagttgcaa ttttctgtgc tggtgttggt acattgatat
     1201 ttcatttttg tacagaagga aaagtacctg cctttttagg ttcaagtttt gcgtttatac
     1261 cagttatact agcagctaaa gaggcatatg gtggagattt agcatatgct caaggtggta
     1321 taatagtagc aggattaatt tatattataa tgtcgattat agtaaaagta gttggtgtaa
     1381 ataaaattaa attatatttt cctgcacaag ttacaggagc tatgatagta gttataggat
     1441 taaatttatt gccaacagct tttagtatgg cttcagccaa ttttgtgata gcatttataa
     1501 ctttagcaat agcaatttta accaataagt ttggtagagg atttataaaa caattaggaa
     1561 tattaatagc agttttttct gggtatataa tctgtttgat attaagatta gtagatataa
     1621 ctactattac agaagcaagt ttatttgcaa tacctaactt tacagttcct aagttcagtt
     1681 taggagcaat agtgattata tctccagttg tattagctgt atttatggaa catataggag
     1741 atatgactac taatggtgct gtagtaggta aaaactttat agaaaatccc ggattaaata
     1801 gaacattact tggggatgga tttgcgacta tagttgcagg gtgtttagga ggaccagcca
     1861 atacaactta tggagaaaat acagcagtac ttgctataac taaaaactat gacccttcaa
     1921 tacttagacg tacagcaata tttgctatat tgcttgcatg tgtaggcaag tttggtggat
     1981 ttcttcaaag tataccaggt tcagttatgg gtggaattag tataatgcta ttttcgatga
     2041 taacttatgt aggattaaaa actataaggg acagttcttg tgtagaaagt aaaataaata
     2101 tattaatcat agcagttata ttattaatag gtcttggaac aacatattta tctaataaag
     2161 gtatttctgt tggaatccct ataacaagta cagtaaagat aactggtctt agtttagctg
     2221 caattgttgg aattgtatta aacagaattt taaataatca ggactttaaa gttgaagaag
     2281 aataaattta aagaataagt tgtatattga ataactaaaa ttagttaatt ttctatagac
     2341 atttgtaatt aatgttgata taatgattaa aaataaatga aagaaagggc aaggtaacta
     2401 ggacaatgag aatataatct attttttata atttaaagta ttgtaatttt tgaaaagcct
     2461 attttggtgg ctttatttgc aatgcttaaa ttaaggagag tagattatag tctatacgtt
     2521 tatttgttat gcctatttat agaatctctc cttatattta agtgtgttaa ggagggattt
     2581 tttatgttgt tagtaaaagt agagaattta aagaaatatt atgcagataa attaattttg
     2641 gatatagata aacttgaaat attagaaaat gacaaaatag gtctagttgg ttcaaatggt
     2701 caaggaaaaa caacattatt aaaagcaata ttgggagaaa tagaaattga tgaaggatat
     2761 acttacctta cagaaagcta ttcttatata agtcagagtg aaaataatat tgaaacatgt
     2821 agtcatagta aggagaagag tcttttaaat gctccagata aatttgaaga acacttatca
     2881 ggaggggaaa aggttaagtt aaaaatagca gatgcgctga gtaataagaa aaatatcata
     2941 atagctgatg agccaacttc aaacttagat aaaaaaagca ttggggtttt ggaagatatg
     3001 tttaaaaggc atgagggagc attattatta atatctcatg atagacgttt tctggatgag
     3061 ttatgtacaa ctatattaga actagaagat ggaaaactaa aagcttacaa aggcaattac
     3121 actgattatt taatgcaaaa agatgaagaa gtaaaaagag ctgattttga gtatcaagaa
     3181 tatgttaaag agaaaaaaag gcttgaaaaa gctcttttat ataaaaaagc tttaagtgat
     3241 ggcataagaa aaacaccaaa aagaatgggt aattcagaag ctaggttgca caaaatgggt
     3301 ggtcagacta ataagaaaaa gttggactca aatgtgaagg ctataaaaag cagaattgat
     3361 aaacttgagg taaagaataa acctaaagtt tctaaagaaa tgaacattaa gatgcaagat
     3421 ggtatggaga taattagcaa aaatctagta gaggtaaaag atatgactct aaagttagaa
     3481 aataagctcc tgttagataa tgtttccttt aagataaaaa gaggtaaaaa gatagcattg
     3541 ttaggtgata atgggtgtgg aaaaagtact ctgataaaag aaatattggc tgataaaaat
     3601 gacaatataa aaataaataa caaggtaaaa gtaggttatt ttgaccaaaa tcaaagttta
     3661 ttagatgagg aaaagagtgt tctatacaat actaaagtta atagttcatt tgatgaatct
     3721 tttataagga taaacttaag cctatttggg tttaaaggtg atgatgtcta caaaaaagta
     3781 aaagtactta gtggtggaga aaaggtaaaa atagcactat gtaaaataat attggaagat
     3841 aataattttt tagtatttga tgaaccaaca aattacttag atataaaatc tatggaagca
     3901 ctggaaaagg cactgataaa tactgataaa actatgctta tagtgtctca tgatagagta
     3961 tttgtatcac atatttgtaa ctatattata gagataaaag atgctaaaat aagggagttt
     4021 gattgtaatt atgacgaata tacaattagc agaaataaaa aaactcctag tagagaaaac
     4081 caaatcaaaa aagaaaatct tctagtatta gaaaatagac ttacaactgt tatatctatg
     4141 ttatctatag aaaaagataa tctaaaaaaa gaattgtatg aatcagaata taatgaatta
     4201 ctaaaacaaa tcaataaatt aaaaaatagc ttttagtgtt aactttttaa ttacatctat
     4261 ataagaagac tgaaaatatt tgttgggaat ataagtatag ttattaaaac agaaaagtat
     4321 tttgttagaa aattaaaagt aagctaatta gaaagtggcc ttattattgt actttataag
     4381 aaggccacta tttttattta gaatgataat gtgtagttgt ctaacattta ttttgtattt
     4441 agtgcagaat ttattcagat ttaaccttaa gcttggctac tgtttcttct tcaggagtaa
     4501 cataaatagt actgttagtt tcataaataa ccatacctgg tttagaacca ctaggttttt
     4561 tcacattttt tctcttagta tagtctacag gcacctgaga agaaagtttt gacttactaa
     4621 agaatgctgc tagcatagct ccttcaaata cagtattatc agggacttcc ttaccagcac
     4681 actttattat tacatgagaa ccaggaatat tttttgtatg catccataag tcatcattat
     4741 cagcaagtct tagtgtaaga tagtcatttt gcttattgtt tttaccaacc aatattttaa
     4801 atccatcaga agacacaaat tcatgaggtt ttgtagatgg gatagtatcc tttttactgt
     4861 ttttcttttg agtcttgatg tatccaactt tagcaagttc ttcttttata tcttgtaatt
     4921 cagctaaatt ttcacagttt tcaatactta aaattatgtt ttctaagtaa tctatttctt
     4981 ccttatttaa ggaaatttga tgagatatct cgactttagc atgtttcatt ttgttatact
     5041 ttttaaagta tttttgagca ttttcagatg gtgtcaagtt tttatttaat tctattgtta
     5101 catcattaca attttcatca tagaagttag caacttcaat actttccata cctttttcta
     5161 ccatataaat atatgaagta atcaattctc cttttatctt ataaatgtct gcattttcgg
     5221 attctgataa ttcttcttct tgttttttaa gtttattata aagtctatca agcttaacag
     5281 atatactctt ctttaaatca gatgaacgtt gattaattct atctttaata tccttagttc
     5341 tatagaaatc ttccagaata cgagacatac tatccttatt tatatatgat aaatcgctaa
     5401 ataatgtaag gtttatacaa ctgaaatcaa ctaccttatc caccttttta tctataataa
     5461 tgcaaggaga gtatttgtta gaatttatat cattgaataa attacaaaat actttgtgta
     5521 aagcatcaaa ttgttcatca gatatatcct ttatgatagc attttgattt acacctgctc
     5581 tgtagcaaat ttctttagct ataatagggc ttatacctag aaatttagaa tatatagact
     5641 taaagatagg tccttccaat tcctctaggt ttttaataaa caaatcctta gatatatcat
     5701 caagagggtt gagtttgttt tgttctggtg gcaatgaata atcatgacca ggtaaaactt
     5761 gacgaactct acttatgcta aaagggactc tttttataga atctataatt ttattttctt
     5821 caccatgagt tattattatg ttgctatgtc ttcccataat ttcaatcata atatttttaa
     5881 ctgtcttttc ttttaattca tctaaagatt caacacttat ttttattatt ctttcaaaac
     5941 ctatttgaga aatttctact atatttccac cttgtatgta ttttctaagt aacatacaaa
     6001 acataggcgc ttttagaggg ttttcttttt gataattact tgtaagataa actctaggat
     6061 ttgaggcact tgcacttaaa accaatctaa aattttcttt attatttctt atattaaaaa
     6121 ttacctcatc atcttcaggt tgatgtatct tatctatttt tccacctgta agttttgatg
     6181 ataattcatc gactattgaa tgtataacta atccatctaa tgccatttat agcacccctt
     6241 tctttgacca aaatgagtca gtaaattaat tatacacata atatacgctt tgtgactagg
     6301 gggaatttaa acaatagaga ttaatattta aattgtataa ttatttatat ttattagaga
     6361 ggaaacttta tttttaaaat taataattta aagtagatta aatataaagt aaatatagta
     6421 taattattgt gtagagtata agctatgaaa gtatatgaac tttacagaaa aaatcaattt
     6481 taaggttcta tagtatataa atatgaaaga aggttgaaaa aatgagattt atcgatttaa
     6541 gaagtgatac tgttactatg cctactgacg aaatgagaga agctatggca aaggctcctg
     6601 ttggagattc aatctttaga gatgacccta ctgtaaataa acttgaagaa ttagcagcag
     6661 ctaaagttgg aaaagaagat gctattttcc ttccaagtgg aacttttggg aatcaattag
     6721 ctctatttac tcattgccta agagggcaag aaataattat tggtaaaggt tatcacattg
     6781 tgactcatga ggttggggca cctgctgtaa tagctggagt tcagcttaga actatagatg
     6841 aagacgaaag tggagctcta aatcctgaac ttgttgaaaa ggcaattaga agagatgaca
     6901 tacatgaacc acagacaggg cttatatgtg tagaaaatgc atattcaggt ggaacagttg
     6961 tgagtcttga taatttaaga gaaattaaaa aaatagctga gaaacataac ttaccagtac
     7021 atttagatgg agcaagatta tttaacgcag cattaacttt gggtgtagaa gctaaagaaa
     7081 tagcaaagtg ttgtgatagt gttatgttct gtgtgtcaaa aggtttagct gcaccaatgg
     7141 gttctatact tgctggaaat aaagaattta tagcaaaagc tagaagaaag caaaaagtta
     7201 tgggtggggg aatgagacaa gtaggtatag ttgcagcagc tggtataatt gccattgaaa
     7261 aaatgacttt aagattgaat gaagaccatg aaaatgcaaa atatttagct tgtgaattaa
     7321 ataaaattga tggcattgaa gtaaacaatg ttaatccaga tataagtatg gtatttttta
     7381 agatgagtga agatattata aaagaggatg ttttaataaa agaatttttt gaaagaaata
     7441 taaaaattaa taaaatggaa aatggtgaat atagatttgt aactcatgta gatattacta
     7501 aggaagattt agactatgtt ttaaaaacac ttaaagaatt aataggtgta tgttaataga
     7561 tatctactag aaggattata gctagttacc agatgtttaa ttaaaaaatt catagacagt
     7621 ttaaataaaa catacataaa attcatatag aaatattaat atataaacat agaaagaact
     7681 agcttaaaaa cataatataa ttaccctaat tgtaatggca actctccctc acatcctcaa
     7741 attatagttg tcattacaaa acttttccca taaattataa gtttatggga atttttttat
     7801 gttataatag tttaagcgtt aatttacgaa ggagcgatac ttatgaaatt ttggaaacta
     7861 catggaatag gaaatgattt tatagcaata gatggaagat tcgaccagat aaatccatct
     7921 gattatactg ctttagcaaa aaaagtatgc catagaagat tttctgtagg agcggatgga
     7981 ttgttagttg tgaaagattc aaaagttgca gatgtagaaa tggtatatta taattctgat
     8041 ggttcaagag cagctatgtg tggaaatggc cttagatgtt tttgtaaatt tgtttatgat
     8101 aattgtatag ttaaaaaaga aagttttaca gtagatacat tagatggtat aaaagatata
     8161 aaagtaaatc ttactaatag agaaataaac tctataagag tcaatatggg aaaaggaagt
     8221 tttatagcta aggacgttcc tgtacttaca aataaagaac gttttgtgca agaaagtata
     8281 aaggttttag atagggagct taaagttact tctatgttga tgggggttcc tcatacagta
     8341 gttatagtag atgaattaga tgtagatgat gtttgtaaat atggaaaaga gattgaaaat
     8401 aataaaatat ttcctgaaaa aacgaatgta aattttgtaa aggtagaaga taagtataat
     8461 atacatgttt atacttggga aagaggttgt ggatatactt taggttgtgg tactggtatg
     8521 actgcatcag caatagtgtg caatttactt aatatggtag aaagtagtgt aaatgtaacg
     8581 tctcaaggtg gaacagttaa aatagatgta ggagatttct cttatatgac aggtccagca
     8641 gtaaaaatat gtgaaggtat tttggaggtt taattaatgg ctataagtat gactggtttt
     8701 ggaagaggag aatacaagga tgataattat tactttttag tagagtgtaa aacgataaat
     8761 cataagtatt cagatataaa catacgtctt ccaagaaaaa tttcattttt agaggataaa
     8821 gttagaaatt tagtaaaaaa ttatgtaaaa agaggaagag tagatttata cataaagttt
     8881 gacttattag gcaaagaaga tgtaaactta aattttgatg agggattagc atctcagtat
     8941 atagacatac taaaagaaat aaaaaataaa tttgatatta tagatgatat tagcgttatg
     9001 aatgttgcaa agtttcctga tattgtgaaa atagaagaaa aggaagaaga tgaagattta
     9061 ttgtggtcaa tgttgaatca agcagttgaa gatgcattaa tcaaattaag agaaatgaga
     9121 agtgaagaag gtaaaaaatt agctgaagat attgccatga gatgtgattt attaaaaaat
     9181 catatagaag agattgaaaa atattcaagt agtgttgtag aagattatag agaaaaattg
     9241 aatttgagaa taagtgaatt actggatgac ccaagtataa tagatgagaa tagattagca
     9301 caagaagttg caatatatgc agataaaagc agtataacag aagaaatagt tagatttaaa
     9361 agtcatatag ggcaacttaa aaatactata tttaaagacg actctatagg tagaaaaata
     9421 gatttcttaa tacaagagat gaatagagaa actaacacta taggttcgaa gtcatctgat
     9481 ataaatataa ctaatttagt agtagaagta aaaagcgaat tggaaaaaat aagagaacaa
     9541 atacaaaata tagagtaaga atacaatatt agaaggggaa tgcttaaatg tttgtgaaaa
     9601 aaggattatt gctagttgta tcaggaccat caggtgcagg aaaaggtact atttgcaaag
     9661 aactacttaa agaaaatgat acaataaaac tttctgtatc agctactaca agaaaaccca
     9721 gaactggaga agttgatgga gtaaattatt tcttcataag taaggaaaag tttgaagaga
     9781 tgatagaaaa aggtgagttt ttggagtatg ctcaaattta tgataatttt tatggaactc
     9841 caaaagcggc aataatggaa tgtttagaaa aaggacaaga tgtattactg gagattgaaa
     9901 tgcaaggagc aaaacaaata aaagaagttt gcccagaggg agtatttata tttgtactac
     9961 caccatcact tgaagaatta aaaaatagaa ttgttggaag aggtacagaa acagaagctg
    10021 aaatagagaa gagatttagc tgtgcatatg aagaaattaa aatgattaag gattatgatt
    10081 attttatatt taatgaagat gtaaaaacat ctgctaaaga aatagaaggt ataatatcat
    10141 ctgaaaagaa taaagttagc agatataaaa atattataat agagaaattt aaggaggaat
    10201 tataattatg ttaaaaccat ctatcaatga ggtactagaa aaaatagaca acagatacta
    10261 tttagtgggg actgtttcaa agagagcaag aaaattaatt gatggagaag aaccatatgt
    10321 ttcaaataaa actaaggaaa agcctgtatg tgtagcaact aaagaagttg caagtggaaa
    10381 aataacatat agattattga cagaagagga aatagagata gaagaagcta gacatcatgc
    10441 tgagcaacac caacaaattt cagaagagga gtaaggtcat gctaaaagat aaaactgttg
    10501 taattggcat aagtggcggt atagctgcat ataaggcttg tgatgttgta agtaagttaa
    10561 aaaaactaaa tgcaaatatc catgttataa tgacaaaatc agcaacagaa tttgttaaac
    10621 cacttacatt tcaatcgctg agtcaaaatt atgtagtaga agatatgttt gaagaaccaa
    10681 aaacttggga agttgaacat atatcattag caaaaaaagc agatttattc ttaatagtgc
    10741 cagcaagtgc aaatgttata ggcaagattg caaatggtat agctgacgat atgttgacaa
    10801 ctactgttat ggcaacaaaa gcacaggttc ttatagcacc agctatgaat actaatatgt
    10861 atgaaaatcc aattgttcaa aaaaatatac aaactttaaa gagctttgga tataaattta
    10921 ttgaaccgga aagtggaagg cttgcatgtg gagatattgg aacaggtaaa ctagctaatc
    10981 cagatataat agtagaggaa gttttgacta atcttactaa agagcaagat ttaaaaggtc
    11041 aaagtatgat agttacagct ggtccaactg tagagagtat agacccagta agatatttaa
    11101 ctaataggtc ttctggtaaa atgggatatt ctatagcaag agaagctata gctagaggtg
    11161 ctgatgtaac attaatatca ggtcctacaa atctttctat tccacaaaat cttaaaaaat
    11221 ttgttcaaat agagtcagca caagatttgt atgaagcagt agtatcaaat ttaagtgaaa
    11281 ataaaatagt aattcaaagt gcagcagttg cagattataa accaaaaact tattctgaca
    11341 aaaaaattaa aaagaagaat gatgatttat caatagaact aagtagaaat tatgatattg
    11401 cttatgaaat aggaaaaata aaagaagata agatactaat tggatttgca gctgaaacta
    11461 atgaccttat agaacatgcc aaaggaaaaa taaaaaagaa aaatttagat tttattgtgg
    11521 caaatgattt aactaaagaa ggtgctggtt ttagagctga tactaatatt gtaaaaataa
    11581 tagatgtaga gggaaatatt actgagtatc ctaagatgaa aaaagaagaa gtagccaatg
    11641 ttattttaga taaagttaaa gcgttgcttg agaaataaga ccttttttaa atatgatatt
    11701 tgtgtatttt atcacagcta aagtggctga aagctgcaat ttaattttag cgagtaaaaa
    11761 ctcgcaaatg tcagttaaat tgtagttttc agcatttatt ttgtcataaa gtataaaata
    11821 taattaatag ttaattatta aaataaagtt aatattaata acttgaaatt gcttataaat
    11881 tctgagattt taatatcttg taaagtatat taaaatatga acaatatatt aaatttaagt
    11941 agaggtttta taatgaaaaa atatgcaaag gtaatagtta gaagtaatac tatatataca
    12001 gataatttat ttacatatca aattcctgta tttttatctg atgtaataaa aattgggcac
    12061 agaatattag taccatttgg aaaaggtaat aaaccaacag aagcctttgt atttcaattt
    12121 acagatagct tagatgaaaa aataaaaata aaagaaatta ttgatatatt agatgaaaat
    12181 ccgatattta gaaaagaaga tttagagttg gtttattgga tgaaaaatag atatttgtgt
    12241 acttatatag aatgtataaa tttgatatat ccaaagggat acaaattaaa taattataaa
    12301 gtagtcctat taggtgaaag tttaagtgga ttaaatgata ttgaattgaa ggaaaaaata
    12361 tcaactttaa gtgataaaaa tagagaaata gtagagaaag tgattgatag taaaggtaaa
    12421 attaaggttg ataagttaaa atatatacca aacttaaata gttctttgta cacaatgaat
    12481 aaaaatggaa ttataaaatt atgttgggaa tataaaaatc acaaaaatga aaagaaagta
    12541 tgttatatat ctttgagttt agaaagtgat aagattgacg attatataga gcaaaataaa
    12601 ataaatgtgg gaagtaaaca aaaagagata ttaagttttc ttaaaaataa tgaaaatgta
    12661 gagataaatg atttattgga tttattaaat gcttcaaaac aaagtataaa ttctctaagt
    12721 aagaaaaagc taataaccct agaatttaag gattattata gagagccaaa aagtatttac
    12781 aaatccgtac taaaaagtat aaaattaaac aatgagcaac aagaagcagt tgatgagata
    12841 aagtcgaata tgtttgtaga tgataaaaaa ccatacttaa ttcatggagt aactggaagt
    12901 ggaaaaactg aagtttacat ggaaataata gaatttgctc taaatcaagg cttagacagt
    12961 atattcttag tacctgagat agcattaact cctcaaacaa tagataggct aaagagtagg
    13021 tttggagatt tagttggagt gttccacagt aaactatctg aaggagaaaa acatgacgtt
    13081 tacaaagctg tcaaagcagg taaagtaaga gtgttaattg gagctaggtc agctttattt
    13141 gcacctttta attccttagg cttaataata attgatgaat gtcatgagtc atcatataag
    13201 tctgagaaga atccaaaatt taatgctata gaagttgcta gatttatggc attaaaaaat
    13261 aatataactc ttattttagg ttcagcaacg ccctcaattg aagagtatta tagggcaaaa
    13321 tctggagaat acaaacttat aaatataaaa agtagagcaa atgataagcc tcttccaaac
    13381 attgaagttg tagacatgaa agatgagtta gataaaggaa atagaagtat atttagtttg
    13441 aaattacaaa aagaaatacg ttatgcgatt gaagaaaata atcaagtaat actattttta
    13501 aacagaagag gttatgcaaa ttttgtatct tgtagaaagt gtggatatgt gtttcaatgt
    13561 gaaaattgtg atatatcttt aacttatcat aagaaaagca atactggaag atgccactat
    13621 tgtggatatg agaaagaaat tccaaaagag tgtcctgaat gtaaaagtac ttatgttaag
    13681 ccatttggag ttggaactca aaaaattgaa gaggaattaa aatatatttt tccagatata
    13741 aaaactttga gaatggataa agatacaact tcaaaaaaag gtgctttaga tgagatacta
    13801 aataaattta aagataaaga agcagatgta cttattggaa cacagatgtt aagtaaaggg
    13861 ctggattttg agaatgtaac tttagtaggt attttgtctg cagacatgat attaaatttt
    13921 ccagacttta aaagttttga aactacattt caattgataa ctcaagtctc tggaagagca
    13981 gggagagctg ataaagaggg aaaagtagta cttcagactt atgacactga acactatgct
    14041 attaagcatg ctattgaata tgattatgag ggtttttatg aagatgagat aaaaataaga
    14101 aaagcatttg gatattctcc ttttaataat atgttaagtg tagttgttag tggtgaagat
    14161 gaaagattag tgataaaaaa tataaaaaat atgcatgcat cattgattta tttattagaa
    14221 aaaaggggta taaatgattt aggatttata ttaggtccaa atccatgttc aatatcaaaa
    14281 ataaatcaaa attatagatg gcaaatactc tttaaagatg aaaatattga aattaatctc
    14341 ttaaagggta taataaaata tatatgtata acaaaacgag atttgatatt tgataagaat
    14401 atcaatgtat ctatagatat taatccaaac agtgtattat aaatttagga ggaattataa
    14461 tggctttaag acaaatagtt caaataggag aaccagtatt aagaaaaaaa tcaaaaaagg
    14521 ttgaaaaaat tgatgaaaag ataatacaat tattagatga tatggcagaa actatgtatg
    14581 atgcagatgg tgttggactg gctgcacctc aagttggaat attaaaaaga gtagtagtaa
    14641 tagatattgg agaagagctt atagaactta taaatcctga aataatagaa acatctgggg
    14701 agcaaataga tgaggaagga tgtttaagtg ttgttggaga agcgggaaat gttagaagac
    14761 caaattatgt aaaggtcaga gctttaaata gaaatggtga aacaatagag ttagagggag
    14821 aagaactttt agctagagca ttttgtcatg agatagacca cttagatgga attttatttg
    14881 ttgacaaaat agaaaagtag taggagttga tttgatgaaa atagtattta tgggaactcc
    14941 tgatatagca gttccatgtt tacaaaaaat aatagatgag aagtatgaaa tattgggtgt
    15001 tgtaactcaa ccagataaac caaaaggaag aggtaaaaaa ctaggtatga gtccagtaaa
    15061 agaactggca attgaaaata atatacctgt atatcaacca gtaaaggcta gagataaaga
    15121 atttatagat aaaattaaat ctttaaatcc agatgtaata gtagttgtag cttttggaca
    15181 gatacttcca aaagaaatat tagagattcc taaacttgga tgtataaatg ttcatgtttc
    15241 tttacttcca aaatatagag gtgcggcacc tataaattgg gtaataataa atggtgaaga
    15301 aaagactggt gttacaacta tgtatatgga tgaaggatta gatactggag atatgatact
    15361 aaagacagaa gtaaatcttg atgaaaatat aacagcagga gaacttcatg ataaaatgat
    15421 gaatatagga gcagaaactt taaaagaaac attaagatta attgaagaag gaaatgctcc
    15481 aagagaagta caaaatcatg aagaattttc ctatgcgcca ataatgaata aatcattagg
    15541 taacatagat ttttcaaaaa gtgccagaga aatacacaat ttagttagag gagtaaatcc
    15601 atggccaagt gcttatacta cctataatga tgtcataatg aaagtttgga aaactaaagt
    15661 gttagatgaa aaaagcacaa aagatgtagg tacaataatt gatgtaagca aagatgggat
    15721 aaaagtaagt actatagata atgtactttt aattgaagaa attcagatgc cgaataaaaa
    15781 gagaatgttg gttggtgaat atataaaagg gaatactata gaaactggtt tagtattggg
    15841 ttaggtgaaa agtatgacag atataaagaa gtatttaaca agtgttggga ttttgatttt
    15901 aatattagga attttgatag gagttgttaa tttttttata ataagtttaa ctcaaatatt
    15961 ctccttgatg ataaatatta taataatatc actaatactt ataatactat gttcaacagt
    16021 agttacttat agagtaatta aaggaaagta tgttaactca aatataatga aaataaattt
    16081 taatattgtc agtggattat ttcctattat atctttcatt gcatcatcat ttggtatgtc
    16141 aaaaagtgat attagaagaa tatatataaa gttaaataat gaatatatat acagtaataa
    16201 atataatttt aatccagagg atataataat tttaatacct cattgtattc aagagaatag
    16261 ttgtaaatta aaagttacta atgatataga taattgtaaa gaatgtggaa gatgcaacat
    16321 aggagaatta attaagctaa aggaaaaaac taatgtaaaa atatttgtag ctacaggagg
    16381 aactctagct agaaaaatta taatggacac taaacctaaa gctgttgtag ctgttgcatg
    16441 tgaaagagat ttgacatctg gaatacaaga tattaagaaa attcctgttt taggtgtatt
    16501 taacaaaaga cctaatggac cttgtgtaaa tactaatgta gacatgatgg atattgaaaa
    16561 agctataggc tttttaacag gaaaaaatat attagtttgt aattaaactg tttttaagtt
    16621 aagtgaatat taagaggatg tataataaaa atatatttta ggagagtgtt atttatgtac
    16681 cctgtatatg gcggtttttg gggatttgac ccaacaatgg ttgtattaat tccagctata
    16741 ttacttacta tatatgcaca atttaaagtg agttcaacaa caaataagta tttaagagta
    16801 aatacacgtc gaggatatac tggagagcaa acagcaagaa gagtacttga ttcaaatggt
    16861 ttgtatgatg taaagataga aatggttaga gggcatctaa gtgaccatta tgacccaaga
    16921 agaaaggctg taaggttgtc agaagatgta tattatggaa catctataac ttctgttgca
    16981 gtagctgcac atgaatgtgg tcatgccata caacatgcta aaggttatgc acctcttcaa
    17041 ataagaagta gtttagtacc agtagtgaat tttgcttcaa gtatatcttg gtttttaata
    17101 tttttaggat ttattatggc tggtccattt ttaaaaattg gtatactatt gttttcggct
    17161 tcagtgttat tccaaataat aacattgcct gtagaattta atgcttcaag tagagcaatc
    17221 gttcaacttg ggaatttagg tattatagat gaaagtgagt caagacaaag tagaagagta
    17281 ctttcagcag cagctttaac atatgttgca gcagcattgg tttcaatact tcaattactt
    17341 agattgttac taatagctca aagaagaaat gactaaaagt aaagtgggtt gtttagaaac
    17401 aacccttttt tatgtttaat aaaagggaga aatttatatg gatgcaagag aagttggatt
    17461 taaagtatta tgtgatatag aaaaaaatga caattattca aatattgcaa taaataagca
    17521 ttttaaaaat ttagaaataa gtgatatgga tagaggactt gcaacagagt taatttatgg
    17581 agtaatagaa aataaatatt atttagatta tataatcaat aaactttcaa aaataaaggt
    17641 aaaaaaaatg tcaacttatg ttaaaatctt tttaagaatg ggaacatatc agattttatt
    17701 tttagatagt atatcagatt atgcagcagt taatgaaact gtaaaattat ctaagaaata
    17761 tgataaaaaa tcttctggtt tcataaatgc tattcttaga aatgaaataa gagctaaaga
    17821 gactataatg gacataactg aggaagatag tgtaaaatac ttatctataa agtattccta
    17881 taactcttgg attataaaaa actggataga taaatttgga caagaatttg ctgaagattt
    17941 gttggaagca aataatgaaa agcctagtat ctatataaga acgaatacac ttaaaataag
    18001 tagagaagaa cttattgaaa aattaagtga aatgggtatt gtatgtttaa aagttcctat
    18061 ggtagaagaa gctattaaag tcgaaaaaat gaaaaatata gaaaaaaatg aattatttaa
    18121 ggctggttta tttacaattc aagatataag ttctatgata gtagggaaag taattaatcc
    18181 aaaagaaggc tcatttgttt tggatgtatg tagtgctcct ggagggaaat ctactcactt
    18241 agctacttta atgaacaata ctggtcaagt agtagctaga gatatttttg agcataaact
    18301 aaagcttata aactctactg taaatagatt ggggcttaaa aatgtgtttg tagaaagatt
    18361 tgatgcttct gagattgatg aaaatagtat aagtaaattt gattatgttt tagcagatgt
    18421 tccttgctct ggttttggta taataagacg taaaccagaa attaagtata agaaagaaga
    18481 agagttaaaa gacatcactt ctatacaaaa gaaaatttta gaaaatgcat ctaaatatgt
    18541 caaagttggt ggaactttag tatatagtac atgtacaatt caagatgtgg agaatataaa
    18601 tatagttact tcttttgttg aagaaaatca caactttgaa tttgttccaa ttgatacagt
    18661 aaatgtagac ttagacaatc aagataaagg atatttaaag atatatccta atattcatgg
    18721 tatagatggt tttttcatag caaaattaaa gagaataagg tagtgagaga atggaaaaga
    18781 aaaagatagt attaaaaaat tttactgaag atgaacttaa agaatttatg aaaactatag
    18841 atgaaaagcc ctttagagga agtcaaatat tctcttggat atataagggt gcaaaaactt
    18901 ttgatgatat gaacaatata ccaaaaagtt tgagaaataa attagaagag gtttcatgta
    18961 tagggcatat agatatagaa ttaaagttag aatctaaggt agataataca aaaaaatatt
    19021 tgtttttatt agatgatgga aatataatag aaactgtgat gatggattat gatagtagag
    19081 ttactgtctg tgtttctaac caagtaggtt gtaggatggg atgtaatttt tgtgcatcta
    19141 caatggatgg attaattaga aacttagagc catgggaaat tttagaccaa gttattaaaa
    19201 ttcaagaaga tactggtaag agggtatcaa atcttgtatt gatggggagt ggtgagccac
    19261 ttgataactt tgaaaataca aaacaatttt taaaaataat taacgaaaaa aatggtctta
    19321 atatagggta taggcatata actctttcaa cttgtggaat agtacctaaa atgtatgagt
    19381 tggcagactt agaaatagct ataaatttag ctttatcact tcattcacca tatgatgaag
    19441 aacgaagaaa aattatgcct gtagcaaatg cttattctat tgaagaaata ttgaatgcat
    19501 gtagatatta tataaaaaag acaaatagaa gggttacatt tgaatattct cttataaaag
    19561 gggtaaatga ctcagaaaaa gaagcaaagg cattagctaa gctcctaaaa ggtatgctat
    19621 gtcatgtaaa tttaattcca ataaataaag ttgaagaaag agaatatgaa aagccagata
    19681 aagcatttat ttataaattt agggatagtt tggagaagaa caatatacct gccacagtta
    19741 ggatgtctat gggttctgat attagtggag cttgtgggca attgagaaga aaatacaagt
    19801 aatttgtaag gaggaagtcg tatggtttat agttgtgcct cccatatagg aaaaataaga
    19861 aaaaacaatg aagactattg tgagggagaa gttatagata cagaacatgg tccaatagga
    19921 atatttgcca tagctgatgg aatgggtgga cataaaaaag gtgaagtcgc cagtaaatta
    19981 gcagtagaaa atataattga ttttctaaag gaaaacttat tacaacatga taatgtaaaa
    20041 atagactata tagatgatat attaaaacag gcttataata atgtaaactc aattgtgcac
    20101 aaaaaatcta tggaagatat agaatttgaa ggaatgggaa ctacattagt tacggctata
    20161 gtgtataata atgtcttata tgtggcaaat gttggagata gcagatgtta tttgttaaca
    20221 gatgaaaaat ttgataaaat aactatagac cattccgtgg ttgaagaact tatgatggcc
    20281 catgtcatta cagaagaaga ggcaagaaga catccacaga ggaatagaat aactagagca
    20341 attggaacag atgatatggt tgttgtagat atttttaaga aagaaatcaa aaaaagtgat
    20401 ataatacttc ttgcgacaga tggattaact gggtttattg atgatgaaga tataagagat
    20461 ttaatattag attatgaata tgagagaact agtaatataa gtgaagaatt gataagtatg
    20521 gccaatgatg tttctggtaa agataatgtt tcggttatag tgataaaagt ataattagga
    20581 cggtgatatt gtgggagata caattttagg aaatcgatat gaaatcatca ggaagattgg
    20641 tgatggagga atggcatttg tatatgaggc taaagacaga ttattaaaca ggactgttgc
    20701 acttaaagta cttagacctg aatttgtaga tgatgatgaa tttctaacaa agtttaaaag
    20761 agaggcagaa gcagtagcaa gtctttcaca tccaaatata gtaaatgttt atgatgttgg
    20821 tgaagatgga aaagttcact acattgtaat ggagtttgta gatgggaaga atctaaaaga
    20881 gattatacaa gatgaaggta tattagatga atatactgca ttagatataa caaaacaaat
    20941 agctatggca cttagtgcag cgcacaaaaa ggggattata catagagata taaaacctca
    21001 taatattctc atatcaaatg aaggaagagt agtaaaagtg gctgattttg gtatagccaa
    21061 agctgtttca aattcaacaa tgactaatat tggtagtata ataggttcag tgcattattt
    21121 ttcaccagag caagctaaag gtaaatttgt aactaataat gctgacctgt attctctagg
    21181 tatagtttta tatgaaatgt tgataggaaa agttccattt aggggagata gtccaatttc
    21241 tatagcactt caacacatta atgatgatat agactttaca tcagaagaaa aagttaggat
    21301 tcctcaaagt gtaagaacta caataaaaaa gcttacagaa aaatctagtg cagatagata
    21361 tcaaactgct gaagaattga tagaagatat agagtatata gaaaaaaata ttgatttaga
    21421 ttttataaaa gaatatgatg attttgcaac taagaaaatt gatgaaaaag aaattaataa
    21481 agttgtaaat ccaacacttg caaaaccagc tcctgaaaaa gtggttaagc ctgttgaagt
    21541 agctgatttg gatgatgatg aagattacta tgatgatttt tatgaagaag atgatgatga
    21601 agatgaagaa gaggaagaga taatgagagc taaaaagaat cagagaccta agagtactcc
    21661 aagtaagaga actaagaaaa agaagaaaaa acaggaaagc ccaaaatcta gaagaagatt
    21721 aaaagtaatt gcggctgttt tgatactgat attgtgtgct caagtattct tagcatataa
    21781 gtttttattt gctggagggt ttggcaataa atctttgact gttcctaatc ttgtaaatat
    21841 gacattagaa gaagctcaaa gtgctgttga aaaagaagga ttatacctga gtgtaaaaag
    21901 tgaagagtat aatagtgaag ttgatgaaaa ttgtataata tcacagacac ctgaaggtgg
    21961 aagtacaaat gtcaaaaagg gtgatactat aaatgttgtc gtaagtaaag gttcaagtca
    22021 ggcatctgtt cctaatgtag tgggacttac tttgagtaat gcaaaacaac ttattgaaga
    22081 aaataactta aaagtgggaa ctgttaagta tgaatacagc tcaatatata aagaaggtac
    22141 agttttaagt cagagtccag gtgctggctc atcaagagct caggaaggtg atgaggtaaa
    22201 tctatatgta agcaaaggtt cagaaaaatc aaatacacag actccaacag taccagataa
    22261 aaaaccaaca acaccagaga ataatacacc aacagaacct ggttcaaact caggaaacag
    22321 tggtggaaat tcaggaggct caaattcagg aaatagtggt ggaaactcag gcaataatgg
    22381 aagtaactca ggaggttcaa actcaggaaa tagtggtgga aactcaggag gctcaaactc
    22441 aggagataat ggaggaagtt caggaggctc aaactcagga gacaatggag gaagttcagg
    22501 aggttcaaac tcaggagaca atggagatag ttcaggaggc tcaaactcag gagataatgg
    22561 aggaacttca tcaggagctg gcgcaaacat tggtaaaaca gaataaatat taataaaata
    22621 agagctgtcc ttttatagaa aatattttct ttaaagggca gtttgttttg tataaaatac
    22681 tgaattggtt ataatataag gagaaaagta tttatgaaga agaaaacagt tttaattaca
    22741 ggaggagcta gagggattgg taaagctatg tctaaagctt ttgcaaaaga aggttacaat
    22801 gtattagtaa attttaataa gtcagaaaat gaagctaaag agttgtacac tatattaaat
    22861 gaaaaaaatt tttctgtaaa actttttaag gctaatattt caaatagaga agatgtagag
    22921 gatatggtag attattgtat aaaagaattt ggaggattgg atgtacttgt aaacaatgct
    22981 ggtgtaagtc aagataagtt gtttacagat ataactgatg aagattggga taatatgatg
    23041 aatattaatt taaaaggaag cttttattgt tcacaagttg ctttaaaata catgatatca
    23101 gaaaaaaaag gaaatataat taatatatct tcaatctggg gtatatcagg tgcgtcttgt
    23161 gaagtgcatt attctataac aaaagcaggt ataataggta tgacaaaggc attagctaaa
    23221 gaagtaggac catcaaatat tagagtaaat agcatagctc caggtgtcat taatactgac
    23281 atgttatctg gatataatga ggaagatata gacgctcttg tggaagaaac ccctctaatg
    23341 aggctaggaa ctccagaaga tatagcaaat tgtgctattt tcttggcatc agacaaatca
    23401 aattttatca ctggtcaggt aattagtcca aacggaggat ttgttattta aaaaaaatgt
    23461 ggtattatat aaaaatagga aagaataaca cgatatacta ctcataaaca gtatgtgttt
    23521 atataaattt tataaaggga ggcatttgat gctagaagga aaaattataa aggggattag
    23581 tggattttat tatgtagata cttataatgg tatttatgaa tgtaaggcta gagggatatt
    23641 tagaaagcag aaaataacac ctttggttgg tgatagagtc aaaataagca ttgttgatga
    23701 agatgaaaaa aagggtatct tagaagaaat cgacagtaga gatacagaac ttataagacc
    23761 tcctattgca aatgtagata aagcattaat agtattcgca ataaaaaatc ctaaaccaaa
    23821 cttatctctt ttagatagat ttatagtttt ggcagaaaaa gaaaatttag aaactgtaat
    23881 aatccttaca aaagcagatt tagatgataa tgatacatta gaaactgtta aaaatatata
    23941 tgaacttagt ggttataaag tcattcctgt aagcaatatt actaagttaa atatagataa
    24001 agtaaaagaa gaattaaaag aaaatgtagt agtgtttgca ggaccatctg gagttggaaa
    24061 atcaagctta ttaaatgaaa tagatgaaaa ttttaaactt caaacaggag ttgtaagtga
    24121 taaaataaaa aggggaaaac atactactcg tcacgctgaa cttttaaaat tagaatttgg
    24181 tggaatggtt gcagatacac caggttttag ttcccttgca cttgaagata tagaggaagt
    24241 tgaacttaaa gattatttta tagaatttga taaatttaat gattgtaagt ttggttcaaa
    24301 gtgtattcat gaaaatgaac ctaattgtgc tataaaagaa gctgttacaa atggagaaat
    24361 atctaaagaa agatatgata gttatataca gctgctacat gaaataagac aaaataatag
    24421 taggaggtat tagaaacatg attaaattag caccatcaat attatcagca gattttgcaa
    24481 aattattaga agatgtaaga aaagtagaaa gtgcaggatg tgaatatctt catatcgatg
    24541 taatggatgg acactttgta ccaaacatta cattaggacc acttgtagtc aaaagtttaa
    24601 agaaagaaaa tataaatatg gtatttgatg ctcatcttat gatagaaaat ccagaccagt
    24661 atattgaaga atttgtaaaa gcaggatgtg atataataac tgttcaccag gaggcttgtg
    24721 ttcatctaca tagaactata caaaatataa aatcacatgg aataaaagct ggtgtagtac
    24781 ttaatccagc tacaccagta gatacgatta aacatgtact atctgattta gatatggtat
    24841 tattaatgtc tgttaaccct ggatttggag gacaatcatt tattccttgt gttttagata
    24901 agattaaaga gttaaaagct attatagata gtcaaggatt aaacattgat atagaagttg
    24961 atggtggtat aagccctaaa aacgtagccg aagttgtaca agcaggagct aatgtaatag
    25021 tagcaggttc tgcaatattt ggaagtgatg acattcaaga aacagttaat ttatttagaa
    25081 agaatgcttc tcttgaagaa ttagtttaaa tttaataatg aaaatctgta ttgttttaaa
    25141 tggcgaaatt gaagattacg aagttacaag agatattata attaaggaat gttatgactg
    25201 cataatatgt gcagatggag gggcaaatca cacttataaa atggaaataa tgccagatta
    25261 tatattagga gatttagatt cagtagaaga agaaaagata aacttctata aaaataaagg
    25321 tgttaaattt gaaaaatttc catctaaaaa agatgagact gatacagagc tttgtctttt
    25381 tttagctaag acacttaaag caaatcatat tgattttttt ggtgctttag ggggaaggat
    25441 tgaccacact cttgcaaata taaagctatt atattattta aaagaagatg gtatatattc
    25501 taggatactt tctgataaag aagagatgta tatagttgaa aatgaggaaa tttcattgta
    25561 tggaaaccca ggggacacta tttctgtaat agctataaat ggtgatgcta agggtgtaac
    25621 acttactggc ttagaatacc ctctcgataa ttattatatg aaatactctg tacctattgg
    25681 aatatcaaat gttatgttga gtaattcttg taagattaaa gtagagcaag gctgtgtact
    25741 tgtagttaga aatttataaa atttgatttt gacaattggt tttatattgt agagcagata
    25801 caatcaagta aaatatattt atgattgtat ctgcttttta gtatataatt ataaatatat
    25861 aataaacttt gacataaggg ggattttttg tggtagatag tattattttt gatttagatg
    25921 gaacattgtg ggattcaact gaagaagtat gcaaagtctg gcaggatgtt ttaagtgagc
    25981 ataaggaaat agaattaagt gtcacaaaag atttatttag aagtcttatg gggctttctt
    26041 ttgaggaaat agcaaatcgt ttatttccaa atttaagtga agaagaaaga atgaatttat
    26101 tgaatgaatg ttctttgaaa gaatgtgact atttatctga aaatggagct aaattatttg
    26161 atgatataga agatactctt aaattactat ctaaaaaata taaattattt atagtaagta
    26221 attgtcaagc tggatatata gaatcttttt taaaagcaca taaactagaa cagtatttta
    26281 tagattttga atgtcctggt aatactggtt tgcataaagg agaaaataat aaacttataa
    26341 ttgaaagaaa taaattaata aatccaatct atgttggtga tacgcaaggt gatgcaaata
    26401 gtgctaagtt tgtaggtata ccttttatat atgccaaata tgggtttggg aatgtagatg
    26461 tatatgatta ttttatagat tcatttaaag atttgttaga acatgatata ttaaaataag
    26521 atgagtttaa aaattatatt aaaataataa aaaagttatg gggggattta aaaatggaat
    26581 tacaagatac tatttttaaa agacaaagtg taagaaagtt taaaaatcaa gatgtatcag
    26641 atgaagatat tttaaaaatg ataaaagcag caggtgcagc accatcaggc aagaatattc
    26701 aaaactggca ttttgttgtt ataaaacgcc gtgatttaat ggaaaagata gcagatgtga
    26761 taactaaaaa gcaacaagaa atacttgtag aaatggataa agtatcagtg gataaggcta
    26821 atagatttag aaaatttgtt aaaaatttta cactatttta cttaaaagct ccagttttag
    26881 tattagtttt tacaaaggta tataatccat caggatatta tgaattagag cttatagatg
    26941 cacctaagga gactatagat aaattattta taagaaatcc aggtatgcag agtctaggtg
    27001 ctgcaattga aaactttaca ctatcagcaa ttgaacttgg atatggttct tgttggttga
    27061 caagtcaaaa ctatgcagca gatgagatag aagctgtttt agaagcagaa actggttttg
    27121 aaaagggaga gtatttttta ggtgcaatgt tagctcttgg agtgccagag gacaatttga
    27181 agagtccatc taaaaaacca gtagaagaaa tatgtacatt tataaaataa aagtcaagga
    27241 cattttaaaa tcgaagtaaa ttaaaaacaa aataaattaa aaataaatat ttaaaaaatt
    27301 aaaaagtagg ttttattgat attaatttta tatttcaaat catta
//
LOCUS       DAMJVI010000043        27253 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.045, whole genome shotgun sequence.
ACCESSION   DAMJVI010000043 DAMJVI010000000
VERSION     DAMJVI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27253
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.045"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..896)
                     /locus_tag="PC545_001941"
     CDS             complement(<1..896)
                     /locus_tag="PC545_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDF2456709.1"
                     /translation="MKLKHIAIIGSLFPILFSIVLFFGVLMSADSDDENSNFSSGITG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVNVLLAIIQVESGGTAKDVMQSSESLGLPPNS
                     LDTESSIKQGCKYFASLLSSCKNQGINDLNVAIQSYNYGGGYVGYVAGKGKKHTFNLA
                     ESFAREKSGGKKVTYTNPIAVAKNGGWRYGYGNMFYVELVNQYLTVPQVSGELAQKVM
                     NEALKYQGWKYVYGGSNPNTSFDCSGLTQWCYGKAGISLPRTAQMQYDATQHIPLSQA
                     KAGELVFFHSTYNAGSYVTHVG"
     gene            complement(893..3085)
                     /locus_tag="PC545_001942"
     CDS             complement(893..3085)
                     /locus_tag="PC545_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2456710.1"
                     /translation="MKERIQSAFTKKKIFHFLKMALFVVALSLILLSLLGTVAHATGL
                     VDDTINAENLYSKYPLSNYQLDFYVDNSWAWLPWNWLDGIGKSVQYGLYCITNFVWTI
                     SLYLSNATGYVVQEAYKLDFINDMADSIGQSIQTLAGVTSSGFSTSGFYVGFLLLIIL
                     VVGLYVAYTGLIKRETSKALHAVINFVVVFILSASFIAYAPDYIKKINEFSSDISTAS
                     LDLGTKIMLPNSDSEGKDSVDLIRDSLFSIQVEQPWLLLQFGNSNAEEIGTDRVEALV
                     SASPENEDGKTREEVVKTEIESNDNNNLTIPQVVNRLGMVFFLLFFNLGITIFVFLLT
                     GMMLFSQILFIIFAMFLPISFLLSMIPSYESMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMVAFLQIVCFAGIYMKLGDLMSMFSLNANDSQSMGRRIFRRP
                     YLYMAHRARRMERRLAGAVTAGGVAGAVAGSTVSNARTTRNNSAKDNRGNTSSSMGQR
                     AGSKVGAVLDTKNKVKDKANAMKETVKDIPTQTAYAVHSAKEKAKTSVSDFKRGMVQE
                     QQSRQSGRLEKQEQHKKNIADKRMELQKAQEARQARKTDGSATTGATRPHERPVTASA
                     IPKTNVEKTQEVKRPATTSTSKAGEPVKTNSVKERPLSSGTSDGKIPQSAQTVHSKNV
                     EKVVSHETHQNYKAERTTKAQTVEQAKRTTEHTEKNRNLVTKKGQKKK"
     gene            complement(3085..5535)
                     /locus_tag="PC545_001943"
     CDS             complement(3085..5535)
                     /locus_tag="PC545_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456711.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSIRSMQEQSKRLVTGKLKEVAYQKIDEQTEALVSMI
                     GDNQVDYRFFLGFKLMVTEEQLNLKNLKKSAWLTFKEFLHEVNHTLMNDFVSMPNDEI
                     NRYMKMEKLLENKISRRFKVRRLEINDFGYLMEHLYGRDGIAYEDYEHQLPKKKYKKE
                     TLIKYYDLIRPTRCVIEESQRYLRLEHEDKESYVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVENRKALTTVRNKKKELKDLDNHAYQAGSETSSNVVDALDSVD
                     ELETDLDQTKESMYKLSYVIRVSADDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYMGYSVDTGRNVYLQPS
                     LASQGVKGTVTNALASAFVGSLGGGKSFCNNLLVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKDNAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRSVTQSETRGLLHVIDELRCEDTAISRNIADHIDSFTDYDFAHLLFSDGTV
                     ENAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVSMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGEILSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDINEIKQTLEFFGIDKEDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPIQRNEVE"
     gene            complement(5522..5911)
                     /locus_tag="PC545_001944"
     CDS             complement(5522..5911)
                     /locus_tag="PC545_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456712.1"
                     /translation="MKKIRSYTGIWNVEKVLYAINDFNLPFPVTFTQITWFVITEFII
                     ILFGDMPPLSMIEGAFLKYFGIPAFLTWFMSQKTFDGKKPYSFLKSQITYALRPKITY
                     AGKAVKLQKEIFNETITAVRSVSYVPD"
     gene            complement(6029..6532)
                     /locus_tag="PC545_001945"
     CDS             complement(6029..6532)
                     /locus_tag="PC545_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2456713.1"
                     /translation="MIDDMAVYIANLGKYNEGYLVGAWFTFPIDEEDVKEKIGLNEQY
                     EEYAIHDTDNFPIEIGEYVSIDELNEMYEMIEELPDYIVDCLDEFIGHYGTLEEVVEH
                     KDDIYYYPDCETMTDVAYYYIDELQALGDIPPSLQNYIDYEAYGRDLDMGGCFIATSR
                     GMCEIPY"
     gene            complement(6535..7038)
                     /locus_tag="PC545_001946"
     CDS             complement(6535..7038)
                     /locus_tag="PC545_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDF2456714.1"
                     /translation="MQETRVLVETKRTTGEETTSYWFDLPIEVAEFEEKLGVSTESQD
                     YRIIEKLLPYADEVHEHTSVYQLNELDYMYRQLSSDMQEEYTMLHTVYENLEALYICR
                     NVITLYPDCKSMIDVARQKLMNDPTFKHLSEDCQEYYFDFETYASHLQEHGKFLVTEH
                     GIFELPE"
     gene            complement(6957..7253)
                     /locus_tag="PC545_001947"
     CDS             complement(6957..7253)
                     /locus_tag="PC545_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2456715.1"
                     /translation="MNDIFRDMQAKVGCENISDLPSYKRKVWHEMKQLNLADYEEKQL
                     EDFSKYVFGMSCQTLKDVMKQQKGREEQCRKQGYWWKRKEQLAKKQHHTGSTCR"
     gene            complement(7350..7952)
                     /locus_tag="PC545_001948"
     CDS             complement(7350..7952)
                     /locus_tag="PC545_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2456716.1"
                     /translation="MDKVVIYIHGKGGNAEESSHYKPLFSDCDVVGLNYTAQYPWEAK
                     EEFPSLFDLICKHYKSVQIVANSIGAYFAMNALSDKQIEKAYFISPIVNMEKLIADMM
                     YWANVTEDELRDKKEIKTTFGETLSWEYLCYVKENPTTWKIPTNILFGEKDNLTSYAT
                     ISEFTKQTNATLTVMRNGEHWFHTEEQMKFLDEWINKSAR"
     gene            complement(8037..8198)
                     /locus_tag="PC545_001949"
     CDS             complement(8037..8198)
                     /locus_tag="PC545_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456717.1"
                     /translation="MAIVVIGIYLGFKREFSKLIGFLVVALVAVGLVFNAGGVKDVLL
                     ELFNKIIGA"
     gene            complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC545_001950"
     CDS             complement(8365..10275)
                     /gene="ltrA"
                     /locus_tag="PC545_001950"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2456718.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNHILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(11017..11151)
                     /locus_tag="PC545_001951"
     CDS             complement(11017..11151)
                     /locus_tag="PC545_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017143882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDF2456719.1"
                     /translation="MWELLKDLLLVSFGMGIGVVLMCILTVGKEADRCMEEIKEREDN
                     "
     gene            complement(11159..12358)
                     /locus_tag="PC545_001952"
     CDS             complement(11159..12358)
                     /locus_tag="PC545_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDF2456720.1"
                     /translation="MNETDFLTALKEKRCAYGVSQTRLAIMANISREHLSRIEAGKVT
                     LTEDMKHTLLEAVEKFNPNNPMFLLFDYVRIRFPTMDIKHIIKDILKLNIGYMLHEDY
                     GHYKYTEHYHIGDVFVYVSQDEEKGVLLELKGKGCRQFESYLLAQERSWYDFFTDALV
                     EGGVMKRLDLAINDRTGLLDIPELIRKCENEECISKFRSFKSYGSGELVKHNETDKYG
                     MGHTLYIGSFSSEVYFCCYEKNYEQYAKLGIPIEEVPIKNRFEIRLKNERAYYAVVEL
                     LTHYDAELTAFSIINQYIRFADKEPDKRKSDWKTNVRWAWFIGEGRPPVKLTTQPEPY
                     TLERTLNWLQRQVAPTLKMLKQIDKDNQTEYLETIEKQAKLTERHYQIIKQQTATVEE
                     MVANVKE"
     gene            complement(12560..13954)
                     /locus_tag="PC545_001953"
     CDS             complement(12560..13954)
                     /locus_tag="PC545_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456721.1"
                     /translation="MKMILNKGHRIRASDKNLVYHFSIGTLLFVFIAVILLLNMKQLM
                     RTDWEHFSLLDNGLTLSTYNFITLLIATGVCALVAFLYYRFCYDSFKKLLHRQKLARM
                     ILENKWYEADTVQDSGFFTDLQSRSREKIIWFPKIYYQMEKGLLHIRCEITLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTMLYDMIANRITIDEVKAENGCLRLMKNLV
                     WEYDALPHALIAGGTGGGKTYFLLTLIEALLHTNAVLYILDPKNADLADLGTVMGNVY
                     HTKEEMIDCVNAFYEGMVQRSEEMKRHPDYKTGENYAYLGLPPCFLIFDEYVAFFEML
                     GTKESVSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFSSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLAYARQ
                     DGTATCEAKGDGTD"
     gene            complement(14071..14556)
                     /locus_tag="PC545_001954"
     CDS             complement(14071..14556)
                     /locus_tag="PC545_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456722.1"
                     /translation="MAKEKGSFKEFLSAIAPEHQAFVEKLNTKLIEQGCDLVIKEAKS
                     GYTASYQLEKKTVMNWVFRKTGVFARIYGDNAGKYEDIIASLPADMQKKMTTSRDCKR
                     LIDPNACSDTCVKGFVYALNGDTHKKCRNDGMLFVLTNETAEHIAGLVCAEVNVRKSA
                     L"
     gene            complement(14654..15037)
                     /locus_tag="PC545_001955"
     CDS             complement(14654..15037)
                     /locus_tag="PC545_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2456723.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRAENDDGSTSEEITRRT
                     YDLKCSVQERMIQVSIPADIPVKDYPYNAEVELVNPVADTVANASYRGVNVDWYIKAD
                     DIILKNKGGQVGNPQNHAPQGQPKK"
     gene            complement(15052..15378)
                     /locus_tag="PC545_001956"
     CDS             complement(15052..15378)
                     /locus_tag="PC545_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDF2456724.1"
                     /translation="MELKYVVPNMAQTFGNLEFAGENKIEQQRINGRFTPVSRSYNLY
                     SDVQRADDIIVTIPAKAGEKHFKPEQRVKLINPKITADGKAVKGRGFTNYILTADDML
                     PAEESK"
     gene            complement(15614..18658)
                     /locus_tag="PC545_001957"
     CDS             complement(15614..18658)
                     /locus_tag="PC545_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2456725.1"
                     /translation="MKKILKRLCTGVLAFATVVTALPTTLVHAESKQYWTESEERVGI
                     VEKVMNDGSIGSTFNEGHLTVEGEDAYCIDINTDFKNGYKTRADASSRMSADQISDVA
                     LSIEYVKQYTDNHNGISKNHAYLLRQLVVWQRLSVHLGWQCDNVRASYDEIPKATQDE
                     VFAGAKAFVKENKGRYECGGYIYSGEGQELGQFWAKLNVGNAKLQKTSNNTSITNGNG
                     NYSIAGATYGVFSDKDCTKQIATLTTDDSGNTEVAEVKATTVYIKELSAPLGYKVDTT
                     VYSLKVEAGKTATLKVSDTPKVTDTLIELFKIDMETQKANPQGNASLEGAEFTWKYYA
                     GFYNKDNLPAEATRTWVTKTTAETDSNGTTHYITKLADAYKVSGDSFYMQDGKAFLPL
                     GTLTVEETKSPTGYLLDGAYMQANGSEEQIKALYLTQITEDGDLAVLTGSNQFSVSDK
                     VIRGGVKIQKRDLETKDTKPQGSATLKDTAFDIISLNDNAVLVEGKIYKKNEVVKTIH
                     TDIEGIASTSADLLPYGNFRIVEKQAPNGYLTDGAKPIDFTITENGKIVDLTDEAHSI
                     YNQIKRGDIEGVKIGAGTHKRLADVPFRITSKTTGESHVVVTDDNGQFSTSSKWASHK
                     HNTNAGKTSEDGVWFGTSEPDDSKGALPYDTYIIEELRSDSNKGMELIPPFEIVVSRN
                     NLVIDLGTLTDEYEKEITIHTTATSKDGEKTILAGKEVTIVDTVKLDGLTKGTKYQLK
                     GWQMLKEENAEIIIDGKRVESDYTFVADDEEMKVEISYTFNASELGGKNLVTFEELYD
                     LSNPDEPMKVAEHKDIEDDGQTVLITERIIKIHTTATDKDGNKELEAGKDVTIIDTVT
                     LEGLEVGTKYKLVGWQMLKEENAELLINGKRVESDYTFIADSETMKVEVTFTFDATSL
                     DGKQLVTFEELYDLSNPNEPKKVTEHKDIEDEGQTITFKEKPEVPEKPETPTTPEKPS
                     RPTDSPKTGDNTNVMAFLVMLLTSAGGLAGTYLYKRRKMKKS"
     gene            19186..20193
                     /gene="asrA"
                     /locus_tag="PC545_001958"
     CDS             19186..20193
                     /gene="asrA"
                     /locus_tag="PC545_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HDF2456726.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVLIPFKGTFSDTDVIRYK
                     EVNAFEEMEFAKKANFSPKDVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIRDKVKFALVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEVFNGEATEFNVDYVTDNLISVDVPENIDSNEMIGNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVISKEE
                     V"
     gene            20194..20985
                     /gene="asrB"
                     /locus_tag="PC545_001959"
     CDS             20194..20985
                     /gene="asrB"
                     /locus_tag="PC545_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HDF2456727.1"
                     /translation="MNPYIPVSAKILDIVKHTDKEWTFRVNCNTKGVLPGKFYEISIP
                     KYGESPISVSGYGEDYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIVGFKSPKDILFADDLKRWSKKLDI
                     LVTVDGAEEGYSGNVGLVTKYIPELDMKDINNTSIVVVGPPMMMKFTVEEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDDTYICLDGPVFDYSYAKNLID"
     gene            20998..21969
                     /gene="asrC"
                     /locus_tag="PC545_001960"
     CDS             20998..21969
                     /gene="asrC"
                     /locus_tag="PC545_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HDF2456728.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLAVVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTEFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDKDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDEDSIIKIILNTYRYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGIKYL"
     gene            22026..22802
                     /locus_tag="PC545_001961"
     CDS             22026..22802
                     /locus_tag="PC545_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDF2456729.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALVLGIIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     ILCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSVANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            23073..25091
                     /locus_tag="PC545_001962"
     CDS             23073..25091
                     /locus_tag="PC545_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2456730.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYVFSYDLK
                     RAVYVPFGLMYIFMVSIPVGKSEFPMRLSALAVGAVIIMIAQFVMNRNRMKNVGDKEL
                     ISICDELLEKISLLKNTINDDSSIIKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERISLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEINYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKV
                     KSRDEKKAERKSKIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIEMYKGIVKNIIDEVNKSIENVADVYYIKNLLLIPSLIEDRLMLINAI
                     YKDKHQEDFLENQKLLISNMYNLYINVNKNKIKDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKSLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEAA"
     gene            25306..26163
                     /locus_tag="PC545_001963"
     CDS             25306..26163
                     /locus_tag="PC545_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2456731.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKNNNRKFN
                     IKNKIFKGIGILFVLILLGFFIWINKTYEPQKLAKEALVSNSKVEVTVNENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVAASNFARDNKLIEGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNIS"
     gene            26260..27129
                     /locus_tag="PC545_001964"
     CDS             26260..27129
                     /locus_tag="PC545_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2456732.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELKELRKLGVKMIYLGLESGSNEVLEDIKKGFSSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGAISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
BASE COUNT         8254 a         5026 c         5017 g         8956 t
ORIGIN      
        1 attcccacat gggtaacata cgaaccagcg ttataggttg aatggaaaaa gaccaactcc
       61 ccagcttttg cctgcgataa agggatatgc tgggttgcgt catattgcat ttgtgccgtt
      121 ctcggtaaag agataccagc ttttccataa caccattgcg tcagtcccga acagtcaaag
      181 gaagtgttgg gattgctacc gccatacaca tatttccagc cttgatattt caacgcttca
      241 ttcattacct tttgtgccag ttctcccgat acctgcggaa cagttaaata ctgattgact
      301 aattccacat aaaacatatt tccatagcca taccgccagc ccccattttt cgcaacggct
      361 atcgggttgg tgtaggttac ttttttaccg ccagatttct cacgggcaaa gctctctgca
      421 aggttaaaag tatgtttctt tccttttcct gccacatatc ccacatagcc accgccatag
      481 ttataggact gtatcgccac attcaaatca tttattcctt gatttttgca ggaagaaagc
      541 agggacgcaa aatatttaca cccctgcttg attgagcttt ctgtatctaa cgagttgggc
      601 ggtaaaccaa gactttctga actctgcatg acatcttttg ccgtaccgcc actttctact
      661 tgaatgatag ccagtaacac attcacatac tcagaaatac cgtattcttt agcgtacttt
      721 tccaccatag gctgatgttt caagacttct gcggataaat tcatacccgt aatgcccgaa
      781 gaaaagttgc tgttctcgtc gtcgctgtcc gcactcatca gtaccccaaa gaaaaggact
      841 atggaaaaga ggataggaaa cagactgcca atgatagcga tatgtttcaa tttcattttt
      901 tcttctgtcc tttcttcgtt acaaggttac ggttcttttc agtatgttct gttgtacgct
      961 ttgcttgttc aactgtttgt gcctttgtgg tacgttctgc tttgtaattc tggtgtgttt
     1021 catgcgatac cactttttct acatttttac tatgtactgt ctgtgccgat tgtggaattt
     1081 ttccgtcaga tgtacccgaa gataagggac gttctttcac actatttgtt ttgactggct
     1141 cgcctgcttt ggaagttgaa gttgtagcag gacgtttgac ttcctgtgtt ttctcaacat
     1201 ttgtttttgg aatggctgaa gctgtgactg gtcgctcatg gggacgggta gctcccgttg
     1261 tcgctgatcc gtccgtcttt cgtgcctgcc ttgcttcttg tgctttttga agttccatac
     1321 gcttgtcagc gatattcttc ttatgctgtt cctgcttttc caagcgtccc gactgtctgg
     1381 attgttgttc ctgcaccatg ccacgcttga agtccgatac acttgttttt gctttttcct
     1441 ttgcggaatg gacggcataa gcggtctgtg tcggtatatc cttgaccgtt tctttcatag
     1501 cgtttgcctt gtctttgacc ttattcttcg tatctaagac agcacccacc tttgaaccag
     1561 cacgttgtcc catgctggaa gatgtattcc ctcggttgtc ttttgctgaa ttattccttg
     1621 ttgtccttgc attagaaacc gtactgccag ccaccgcacc agcaacaccg ccagcggtaa
     1681 cagcaccagc aagccgtctt tccatacgcc tagccctatg tgccatataa agatacggtc
     1741 tgcggaaaat tcttcgtccc atgctttgac tgtcgttagc gttcaaagag aacatactca
     1801 ttaaatcgcc taacttcatg tagataccag cgaaacacac tatctgcaag aacgccacca
     1861 taaagaacgg gtagtctgtg gaaatgttat aaaacatact ggaaatgcta aatgccaccg
     1921 ttacaatgag cgtaattcct gcccttgtca ttatggtatt aaataccctc acgattgcct
     1981 gctttgccat gctttcatag ctcggtatca tggatagtaa aaagctgata ggtaaaaaca
     2041 tggcgaagat aataaaaagt atctggctga acaacatcat acccgtaagc aagaatacaa
     2101 atatcgtgat acctaagttg aagaacaaga ggaagaacac catacctaaa cggtttacga
     2161 cttgcggtat cgtcagattg ttgttgtcgt tactttcgat ttctgttttc acgacttcct
     2221 ctctggtttt gccgtcctcg ttctctgggc ttgccgatac aagagcttct acacggtctg
     2281 tcccgatttc ctctgcgttg ctgttcccaa actgtaagag tagccacggc tgttccactt
     2341 gaatagagaa taggctgtcc cttatcaagt ccacgctgtc cttgccctcg ctgtcagagt
     2401 ttgggagcat gatttttgtt cccaagtcca aagaagcggt actgatgtcg cttgaaaatt
     2461 catttatctt tttgatgtag tcgggagcat aggcaataaa tgaagccgac aaaataaaca
     2521 ccacaacaaa gttgataacg gcgtgaagtg ccttgctggt ttctctttta atcagtcccg
     2581 tataggcaac atatagtccc accactaaga taatgagaag caggaaaccg acatagaagc
     2641 cagaggttga aaaaccgctt gaggtaacgc ctgcaagggt ctgtatgctc tgcccgatac
     2701 tgtctgccat atcattgatg aagtccagct tgtaggcttc ctgcactaca tagcccgtgg
     2761 cattacttaa atataaactg atagtccaga caaaattagt gatacagtac aaaccatatt
     2821 gtactgattt tccaatcccg tccagccagt tccacggcaa ccaagcccag ctattatcca
     2881 catagaagtc aagctgatag ttggaaagcg gatatttgga atatagattt tctgcgttta
     2941 tcgtatcgtc cacaagcccc gtcgcatgag ccaccgtccc cagaagtgaa agcaggataa
     3001 gggagagtgc caccacgaac agagccattt tgagaaagtg gaaaatcttc ttttttgtga
     3061 acgcactctg tatcctttct ttcatcactc cacctcattt ctctgtatag gcggtctggt
     3121 atcaaaggcg tgtagcagtt cttcaaagac ggggtgtatc tgtaccacgc cgacacgccc
     3181 gtataagtcc tgcaacaagc attgtccgtt ctccaaatca cgaagtcgct tctgattgtt
     3241 ttcgtcctct ttgtcgatac cgaaaaattc taaagtctgt tttatctcgt tgatgtcagt
     3301 agaacggaaa gcgaatttta agccgatatt gtttttcaga ctttcctttg acacgtcccc
     3361 agaagattgc gtaacgaaat acacacctgc ctgcatagct cgtccagcac gcaccagctt
     3421 gttagataaa atttcgcctt gtgccacatt taagaacgcc cacgcttcat caaggtctac
     3481 gattttgaag atagagcggt cgctgtggat aaaatctaag gcaaaggtac taatcacaat
     3541 cagcatggaa accgatagta attcaatggt cgtgtattcc tcaaaagtcg tgtccttatc
     3601 tggcagtaca aggtcggcta cttgaatgat attgagctga ttatctaaac tgatagcatt
     3661 ttccaccgtt ccgtctgaaa acagaagatg tgcaaaatca tagtctgtaa agctgtcgat
     3721 atggtctgcg atattgcgtg aaattgcggt atcttcacag cgtaactcgt ctatcacatg
     3781 aagcaagccc cttgtttcac tctgggtaac agaacgcacc gccttacgaa gtacggggaa
     3841 tttttcgccg tccctagagg aaataccagt taaaaaggtt agtatgtcga ttgccagact
     3901 ttcagcgtct ttcacattct tcataatcac aaacgggtca agaagccctg cgttgtcctt
     3961 gtcgcttgtt aggtttacga tattgatttc atgggcgatt tctggaagtg tttctttcca
     4021 gtttccacgc tctgattttg ggtctaagat aaccgcttga ccgccaaaca aaacagaata
     4081 gtacacaagc aggttattac agaatgactt cccaccgcca agcgaaccaa caaaagcaga
     4141 agcaagggca tttgtaactg taccttttac gccttgactg gcaagggacg gttgcagata
     4201 tacatttctt cccgtatcaa cggaatagcc catatagata cccgtgtttt cccctaactg
     4261 ctgtgtcgca ccaaaaccaa gcccagctaa gaagtctgat tttacatact gcacataatc
     4321 attgatatat cgcttgctgg ctggtagaaa ttcagaatga agccccagca tatccccagc
     4381 aggacgtact aacttcacat tcaaatcgtc ataaaaatct ttgacttcat cacaacggcg
     4441 tttcagttcg tccaaatcat cagcagacac acggataaca taagagagct tatacatact
     4501 ttcttttgtc tggtctaaat cggtttccag ctcgtccacg ctgtctaaag cgtctactac
     4561 atttgagctg gtttcacttc cagcttgata agcgtgatta tcaaggtctt tcagttcctt
     4621 tttcttgttg cggacggtcg ttaatgcttt tcggttttcc acgatttcta cattcatgct
     4681 cgtatcaacg ggaaaggtaa attgctgttg ctggaaatag aagatttcac tagacggaaa
     4741 atcaagctcg cctacaatcg cattgacggt aaaataggac acataacttt ccttgtcctc
     4801 atgttccaat cgtaaatatc gctggctttc ctcaatcaca caccttgtcg ggcggataag
     4861 gtcgtaatac ttgataagtg tttctttctt atactttttc tttggtagct ggtgctcata
     4921 atcttcatag gcgataccgt ccctgccgta aagatgttcc ataagatacc caaagtcatt
     4981 gatttccaga cggcgaacct taaagcgacg ggagatttta ttttccagta acttttccat
     5041 tttcatgtaa cggttgattt catcattcgg catggaaaca aagtcgttca tcagcgtgtg
     5101 gttcacttca tggagaaact ctttgaacgt cagccacgcc gattttttaa ggttcttcag
     5161 attgagctgt tcttccgtaa ccatgagttt aaagccaaga aaaaagcggt agtccacttg
     5221 attgtcccca atcattgata ctaacgcttc ggtctgttcg tctatcttct ggtaagcgac
     5281 ttcctttaat ttccccgtaa ccaatctttt tgactgctcc tgcatacttc ttatagagct
     5341 ttccgtggca atctgcaaag catgaatttt accctcacgg gactgtgcga ttagctgtcg
     5401 gaaactgtca tgcacgataa atttctgttc tgcggataag aaagaatagt tatacggtat
     5461 cagctcatag taggcgaaca cctcattgtc cttgttccag acaaggttgt tgtcaatata
     5521 tttaatcggg aacataactc acactcctta ctgccgtgat tgtttcgttg aatatttctt
     5581 tttgcagttt tacggctttt cctgcataag tgattttcgg tcgcaacgca taggttatct
     5641 gtgatttcaa aaagctgtat ggcttctttc cgtcaaaggt tttctgcgac ataaaccaag
     5701 tgaggaaagc aggaataccg aagtatttta aaaatgctcc ctcaatcatg gaaagtgggg
     5761 gcatatcccc aaacagaatg atgataaatt ctgtaatcac aaaccatgta atctgtgtaa
     5821 aggtaacggg aaagggcaag ttaaagtcat tgattgcata caagactttt tctacattcc
     5881 agatacccgt gtagcttcta atctttttca agttctttca actcctctca taagtttcta
     5941 aatggaaaag gacagccctg tttcagactg tcccttaaag agaaatacgc cgtcagtagc
     6001 aacaatgctt gtcgctgatc ttccagcgtt aatatggtat ctcgcacatt cctcggcttg
     6061 ttgcaatgaa gcaaccgccc atatccaaat ctcgcccgta tgcttcatag tcaatatagt
     6121 tctgcaagct agggggaata tcgccaagtg cctgcaattc atctatgtag taataggcaa
     6181 catctgtcat ggtttcacag tcgggataat agtaaatatc gtccttgtgt tccacgactt
     6241 cctccagcgt cccataatga ccaataaatt cgtccaaaca atccacgata tagtcgggaa
     6301 gttcctctat catttcatac atttcattga gttcatcaat ggaaacatac tccccaatct
     6361 caatcgggaa gttatcggta tcatggattg cgtattcttc atactgttca ttcaagccga
     6421 ttttttcttt cacatcttcc tcgtcaatag ggaaagtaaa ccaagcaccg actaaatagc
     6481 cctcattata cttgccaaga ttagcgatat aaactgccat atcatcaatc atacttactc
     6541 tggcaattca aagataccgt gttccgttac taaaaatttc ccgtgttcct gcaagtgtga
     6601 agcataggtt tcaaagtcaa aatagtattc ttgacagtcc tcggataaat gtttgaatgt
     6661 agggtcattc atcagctttt gccttgcaac atcaatcatg cttttacagt cgggataaag
     6721 cgtaattaca ttcctgcaaa tataaagtgc ttctaaattt tcatacaccg tatgaagcat
     6781 tgtatattct tcctgcatat cgcttgaaag ctggcgatac atataatcta attcattgag
     6841 ctggtacacg cttgtatgct catggacttc atcagcataa ggcaacaatt tttcaataat
     6901 gcggtaatct tgactttctg tactgacacc taacttttct tcaaactcgg caacctctat
     6961 cggcaggtcg aaccagtatg atgttgtttc ttcgccagtt gttcttttcg tttccaccag
     7021 tacccttgtt tcctgcattg ttcctcacgt cctttctgtt gtttcatcac atcttttaaa
     7081 gtctggcatg acataccaaa cacatatttt gaaaaatctt ctaactgttt ttcttcatag
     7141 tcggcaaggt tcagttgctt catttcatgc cacaccttac gcttgtaaga gggcaggtct
     7201 gaaatgtttt cacaaccaac ttttgcctgc atatctctaa aaatatcgtt cattacatca
     7261 ctccaatctg aaatttaggt aagaaaaaag cagtcaatcc taagactaac tgctttgtgt
     7321 gtatggatat gaagttgtca aactgggatt tatctggcag acttgtttat ccactcatca
     7381 agaaatttca tttgttcttc cgtgtgaaac caatgctctc cgtttctcat aacagtaagt
     7441 gttgcattag tttgttttgt aaattcagat atagttgcat aagaagtcaa gttatctttc
     7501 tcaccaaata aaatatttgt tggaattttc catgtagttg gattttcttt tacataacag
     7561 agatattccc atgagagtgt ttctccaaaa gttgttttaa tttctttttt atctcgaagt
     7621 tcatcttctg taacatttgc ccaatacatc atatctgcaa tcagtttttc catattcaca
     7681 ataggtgaaa taaaatatgc tttttctatt tgcttatctg ataaggcgtt catagcgaaa
     7741 taggcaccta tgctatttgc aactatttga acagatttgt aatgtttgca aatcaaatca
     7801 aaaagtgatg gaaattcttc ttttgcttcc catggatatt gtgcagtata gttgagtcca
     7861 accacatcac aatcactaaa aagaggtttg taatgtgaag actcctctgc atttccacct
     7921 ttaccatgta tataaataac tactttatcc ataatagttg tcctcctcaa cttcaaattt
     7981 gtcgctttct tcatatccac attatatcat tctcatatca gcaccacaat aaaattttac
     8041 gcaccgataa tcttattgaa cagctctaac agtacgtctt tcacaccgcc agcattgaat
     8101 accaatccaa cggcaacaag ggcaactacc aagaagccga taagtttaga aaactcacgt
     8161 ttgaacccta agtagatacc aataaccaca atcgccataa gcactaagct ctgggcgttg
     8221 cttaaaaacc atgtgtatat ggataggtaa gttcctgata ttataaatat ctcaaacttt
     8281 tccccccatt cacacggagc gtgcgacttt caacgcactc cgcgttccat cgtgtttata
     8341 ttgaaaagta tttatgtagt agacctatat caagtttaat tcttgattgc cagctaatac
     8401 tctatattta tttagtctta tgagctggac tttatttagc ttcaatagag ctatatattt
     8461 ttgtattgtt tcttctgttt tagcgtgtat tagacggtgt accgcctctt ttaccaaaat
     8521 atgattttta taatcgtcat ttccaccagc tccagttggt acaatatgat gacaatgtat
     8581 ttcattagct tgttgaaatt cttccagagt tatagcacat tttccatgtt gagcagaaaa
     8641 tagcgaaatt cgattgtctg cgtattctat tgtgcgattt tccaaagata atttcattaa
     8701 ctggtacatc agtaatttat caacacttag attggtgtgg ataaattttc ttccctctag
     8761 agtgtagcaa ttcgctaaat acacctttgc cattggtttt ctgtgttgga tatatccaac
     8821 agggaaaata ggttcgccct cgccagcaac ataccggagc attttagatt taccatatct
     8881 tgttgcctcc tttgtcgtaa gttgtctgcc tgtttttctc aatctgcccc ttttgtttgt
     8941 acctaatctg ttagtgaaga tagccattac agctctattc agtttcatac aatccagatt
     9001 tacattcgtt gcaatctgat aataattttg catacctaat accattgaat tataaagtct
     9061 tgtttcttct aattccgttt tcccttttga gggagaagca attttctttg cctgtgatac
     9121 cagcttgtct ttttggtttt tgaattgttt atcacttata tgtgatttaa caactaactt
     9181 ctttcttctc ggtatcattt taatcttgaa accaagaaag tcggagtatc tttttctaac
     9241 atttacaatt cttgtttttt cttgtgatac ttccaatttt aatcgttgtt caatccattg
     9301 agtgattgct attttggctt tttcagcact ttccttgtag cggcagaaaa ctctaaaatc
     9361 atctgcataa cgaaccatat acatttcctt taactttgtt ttcttcattt ctttgtaagc
     9421 gttgctttta catgggctac cgcttttatt gatatgaacc acatagtttt ttgtaacagg
     9481 attttcctgc cattggcttt ctacccaatg gtctaactca ttaagaacaa tgtttgctag
     9541 tagtggggat attattcccc cttgcggagt tcctttctct ggatatgtta ttgtaccgtt
     9601 agtcattttg attggagctt ttaatatcct ttttaaaatg aatatcaagt gcttgtcccg
     9661 tattcccata gcccatatct gttttatcag cttggaatgg ttcacgttgt caaagaaacc
     9721 cttaatatca aattcaatca cataatgtag ctggctgatt tgtaggcggt tataggttgc
     9781 ttttattgca ttttcaactg acctattagg tctgaaacca tagctgtttt cactaaattt
     9841 agcttcgcag attggttcta atacttgttt tatacattgt tgtataagcc tgtcccacat
     9901 acaaggaata cctagaggtc tggttttacc atttggtttt ggtatttctt ttcgcctcac
     9961 tggcttaggt cgatacccat gtggactgcc tgcaacaatg tatcttactt ttttgacaac
    10021 tgtttctgct ggcaatttgc caatatcttt aattattgta ccgtctgttc ctgctgtttt
    10081 actgcctgca tttgctttga tatttcgata tgccagtaat atattttctc tcgataggat
    10141 aatatccata agatgagtga aaatatctcc attcttactt ttggcatata aatcatcaaa
    10201 ggtgttttgc atttcgtaat attcagcgtg tcttaaatct tctacacaca atgtttcatt
    10261 tttattttta ggcataaggc ttcactcctt tgcttgaaat tgagtgagct tttttatctt
    10321 actcgtaatc cttattgaaa caacataata cttttctaat atatacacga cttgagggca
    10381 ttcctccatt ctcattacaa gaatttctta ggttcgccct ctgcttttca gcattagttc
    10441 aactgcttat tgttcttcgt cagtttattc tcttgatgag aacccaatac ctttcctcgt
    10501 tccgataatc ttatctatac atatatatcc ttaggtcact agctataagc ctgctggctt
    10561 gataccgcct gtaacgttat atggcatttc ataacctata ttcttacttt gccacaccag
    10621 ctacgatttc tcgtaccttt gcatttctac aaagttcaaa tttagacccg tacattcgct
    10681 gtttcgtcag ttccttatag aacattctaa ccatagatat tttatagacc cccgacctgt
    10741 tggatacgct acccgcctct ggatagtttt cgatagaaga tatatgttca tcaactatta
    10801 cggtatcttt cagccgtctt taccgagctt caaacattct gagtttcctc atacaatgct
    10861 tgtcggaggc tcagggcagg cgtttcagag cgttacttca tcattccacc tgtcagatta
    10921 tcagttctcc taataaccaa actttttcgt tcttttatta gtgccttagc ttttcactaa
    10981 ggaacgagtc gcacataaat tttgtccgaa attcatttaa ttgtcctctc tttctttgat
    11041 ttcttccata catctgtcag cttctttgcc gacagttaaa atgcacatca agactacacc
    11101 gatacccatt ccaaatgata ccagcaataa gtctttcaat aattcccaca ttttttcatc
    11161 actcctttac gttggcaacc atttcttcaa ccgtggctgt ctgttgcttg attatctggt
    11221 agtgtcgttc ggtcagtttt gcctgctttt caatcgtttc cagatactcc gtctgattgt
    11281 ccttgtctat ctgcttcaac atcttcaagg tcggtgcaac ctgcctttgt agccagttga
    11341 gtgtacgctc cagcgtgtaa ggttctggct gtgttgtcag ctttacgggc ggtcgcccct
    11401 cgccaatgaa ccaagcccag cgtacattgg ttttccagtc gctttttcgc ttgtctggtt
    11461 ctttatcagc gaagcggata tattgattga taatagaaaa tgctgtcagt tctgcgtcat
    11521 aatgtgtcag cagttccaca acagcataat aggcacgttc atttttcaaa cgtatctcaa
    11581 atctattttt tatggggact tcctcaatcg gtatgccaag tttggcgtac tgttcgtaat
    11641 tcttttcata acagcagaag tacacttcac ttgaaaatga accaatatac agcgtgtgtc
    11701 ccataccgta tttatccgtt tcattatgct ttaccagctc tccagaacca tagcttttga
    11761 aagaacggaa tttagaaata cattcctcat tctcgcattt tcgtatcagc tctggaatat
    11821 caagtaagcc cgttcggtca ttgattgcca aatcaagacg cttcattaca ccgccctcaa
    11881 ccaatgcgtc cgtgaagaag tcataccaac ttcgctcttg tgccagcaga tagctttcaa
    11941 actggcgaca gcctttgcct ttcagttcca gcaggacacc tttttcttcg tcctgcgata
    12001 catacacaaa cacatctccg atatggtaat gctcggtgta tttgtaatgt ccgtaatctt
    12061 cgtgaagcat atagccgatg ttgagcttca atatatcctt gatgatatgc tttatatcca
    12121 ttgtgggaaa acggatacgc acataatcaa acagcaggaa catgggattg ttgggattga
    12181 atttttccac cgcttctaaa agcgtatgtt tcatgtcctc ggtcagcgtt acctttcctg
    12241 cttcgatacg gctcaaatgt tcacggctga tatttgccat gattgcaagc cttgtctgcg
    12301 atacgccgta tgcacagcgt ttttctttca aggcggttaa aaaatcagtt tcattcagtt
    12361 gtcatacccc caatcgtgat aattttgtga tattgtgata atttcaaggc tttatcacaa
    12421 aaggcgaaat gtacgaaaaa cccttatttc atgcggtctt gcaaaggttt ttgtggaagt
    12481 ttccgccgta attgtgcccc tctgttagat aatgaggggc ttgtatctgc tggcgtggct
    12541 aacgccacac cagcaacggc tagtccgtgc cgtcgccttt cgcttcgcac gtcgccgtcc
    12601 cgtcctgcct tgcgtatgca agtgagccta tggtctgcaa aaaatcatgt ccttttggaa
    12661 ctaagggtgt gtaaaattca cttatgacgc ttgttcccac atcacaatag ccacgtccct
    12721 tgatacgctt ctggaaaaac tgttttttca catcagaact gaacagcata ccgtaaccta
    12781 attcgctgat acgccccaag ccaacacgga aattaaaatt atctctgata ccgtccgaga
    12841 aatattttgc gtctggacgc tggcaggcaa caataaggaa atatcctgct tgtcgtccta
    12901 acatgacgat tttctttagc tggctaagta ggctcacact ttcttttgtc cccagcattt
    12961 caaaaaacgc cacatattca tcaaagataa gaaagcaggg cggtaaacca agataggcat
    13021 agttttcgcc cgttttatag tctgggtgtc gcttcatttc ttcgcttctt tgtaccatgc
    13081 cctcataaaa ggcattgaca caatcaatca tttcttcttt ggtgtgatac acatttccca
    13141 ttaccgtacc taaatctgca aggtcagcgt tcttcgggtc taatatgtaa agaacagcgt
    13201 tcgtatgtaa cagagcttca ataagcgtca gcagaaaata cgttttaccg ccaccagtcc
    13261 caccagcaat cagagcgtga gggagtgcgt catattccca tacaagattt ttcatcagcc
    13321 taagacagcc gttttctgct ttcacttcat caatggtaat gcggttcgct atcatatcat
    13381 aaagcatggt gtattcgata taaccgtcat gtaacgtctt gtcggtcagc tcacaataca
    13441 agccactttc caatttatcc tctaaccgta agagctggtc ttgatatttt cctaatgtaa
    13501 tttcacagcg gatatgaagc agtccttttt ccatttgata ataaatcttt ggaaaccaga
    13561 tgattttttc ccttgatctg ctttgcaggt cagtaaaaaa accgctgtct tgtacggtgt
    13621 ctgcttcata ccacttgttt tccagtatca ttcgtgccag cttttgacgg tgcaggagct
    13681 tcttgaaact gtcataacag aagcggtaat acagaaaagc gaccaatgca caaacaccag
    13741 tcgctatcag tagggttatg aagttgtaag tggaaagcgt caaaccgttg tctaataagc
    13801 tgaaatgctc ccaatcggta cgcatgagct gtttcatatt cagtagcagg ataaccgcaa
    13861 tgaatacaaa cagaagcgtc cctatggaaa agtggtagac aaggttcttg tcgctggctc
    13921 tgatacggtg tcctttgttt aaaatcattt tcataaatca atcactcctt tctgggtacg
    13981 aaaaaagcag tcaatcctaa gactaactgc tttgtgtgta tggatatgaa gttgtcaaac
    14041 tgggatttat tgttcttaac ttttctgtcg ttataaagct gacttacgca cattaacctc
    14101 tgcacagacc agcccagcta tatgctccgc tgtttcattt gtcagtacaa agagcattcc
    14161 gtcattacgg cattttttat gtgtgtcgcc attcaaggca taaacaaaac cttttacaca
    14221 ggtatcacta caggcattag ggtcaatcag tctcttgcag tcacgagaag tagtcatttt
    14281 cttttgcata tcagcaggaa gcgaagcgat tatatcctca tactttccag cgttgtctcc
    14341 gtaaatccgt gcaaaaacac ctgtcttgcg aaacacccaa ttcattactg ttttcttctc
    14401 tagttggtat gaagcggtgt aaccgctttt tgcttccttt attacaaggt cacagccctg
    14461 ctcaataagt ttggtattaa gtttctcgac aaacgcttgg tgttctggcg cgattgccga
    14521 tagaaattct ttgaatgaac ctttttcttt tgccattatc tttttcctcc acaaatttca
    14581 atttatccgt ttcttcatac acatattata acagttgctt atatccgcta caatgaaaat
    14641 ctagtttgcc agcttatttc tttggttgcc cctgcggtgc atggttctgt ggattgccta
    14701 cttgaccgcc tttattcttc aagataatat cgtcagcctt gatataccag tccacattga
    14761 cacctcgata gctggcgttt gctaccgtat cggctacggg attgacaagc tctacctctg
    14821 cattatacgg atagtccttg acgggaatat cagcaggaat tgatacttgt atcatacgtt
    14881 cctgcacact acatttcaaa tcataggttc ttcgtgtgat ttcttcactt gttgaaccgt
    14941 cgtcattttc tgctcgcacc tcatgtctga gggcagagaa cttcaataca ccgaaagttt
    15001 tttccttgtc taatacaata ccgtttgcta atctcataat catttacccc cttacttgct
    15061 ttcctcggct ggcaacatat catcagccgt taagatatag tttgtgaacc ctcgtccttt
    15121 gaccgcttta ccgtctgcgg taatctttgg attgataagt tttactcgct gttctggttt
    15181 gaaatgcttt tcgcctgctt tcgctggaat ggttacgata atatcatcag ccctttgtac
    15241 gtcagagtac aagttgtagc ttcttgatac gggcgtaaag cgtccgttaa tgcgttgctg
    15301 ttcaatcttg ttttcgccag caaattccaa gtttccaaaa gtctgtgcca tgttcggcac
    15361 tacatatttt aattccatag tgttttacct cgtttctttc ttctttttta agtttgttga
    15421 ttgtgctttc ttattctggg aattagggaa ataccagtaa tcccccagat agtaaagggt
    15481 aaatcaatgc ttcgcaccct tgactatccg tgtgcttgca ggttgttggc agacaagcca
    15541 gaataagtaa acgtctgccg tttgacagct tcggcagaga gcgtgatttt tcttttctca
    15601 taccgttacc gccttatgat ttcttcattt tacggcgttt gtaaagatat gttccagcca
    15661 gtccaccagc agatgtaagc aacattacca gaaatgccat gacatttgtg ttgtcgcccg
    15721 ttttaggact gtctgttggt ctgctcggtt tttctggtgt tgttggcgtt tcgggtttct
    15781 ctgggacttc tggtttttcc ttaaaagtaa tcgtctgtcc ctcgtcctca atgtccttat
    15841 gctcagtaac ttttttcggc tcgtttggat tgcttaaatc atacaattct tcaaaagtta
    15901 caagctgttt gccgtcaaga gaagtagcgt caaaggtaaa ggtaacttcc actttcatag
    15961 tttcgctgtc agcgataaac gtataatcac tttccacacg ttttccattt ataagaagct
    16021 ctgcattttc ttctttcaac atctgccagc ccacaagttt atactttgta ccaacttcca
    16081 agccctctaa ggttacagta tcaatgattg ttacgtcttt tcctgcttca agctctttgt
    16141 taccgtcctt atcggtagca gttgtatgta ttttgatgat acgttctgta ataagtaccg
    16201 tctgcccgtc gtcctcaata tctttatgct ccgcaacttt catgggttcg tctggattgc
    16261 ttaagtcgta taattcctcg aatgttacca gatttttacc gccaagctcg gacgcattga
    16321 atgtataaga aatttccact ttcatttctt catcatcagc aacaaaggta taatcgcttt
    16381 ctacacgttt cccgtcaatg ataatctcgg cgttttcttc ctttaacatc tgccagcctt
    16441 ttaactgata cttagtgcct tttgtaagtc cgtctaattt gacagtatca acgattgtta
    16501 cctcttttcc tgcaaggatt gtcttttcgc cgtctttact ggttgctgtg gtatggatag
    16561 tgatttcctt ttcgtattca tcagtcaaag tccctaaatc aatcacaaga ttatttcttg
    16621 ataccacgat ttcaaatggt gggataagtt ccatgccttt gttgctgtca gagcgtaatt
    16681 cttcaataat gtaagtatcg taaggcaacg cacctttgct gtcgtctggt tcagaagttc
    16741 caaaccatac accgtcctcg cttgttttgc cagcgttggt attgtgcttg tgcgaagccc
    16801 attttgaaga agtggagaat tgcccattat cgtcagttac cacaacatga ctttcgcccg
    16861 ttgtcttgct tgtgatccta aagggaacat cagcaagacg cttgtgtgtg cctgcaccga
    16921 ttttcacgcc ctcaatatcc ccacgcttaa tctgattgta gatagagtgg gcttcgtctg
    16981 ttaagtccac gatttttccg ttttctgtga ttgtaaaatc aatcggcttc gcaccgtctg
    17041 tcaagtaacc gtttggagct tgtttctcaa cgatacggaa attcccgtaa ggcaaaaggt
    17101 cagcagaagt agaagcgata ccctcaatat ccgtatggat tgtttttaca acttcgtttt
    17161 tcttgtatat cttgccctca accaataccg cattatcatt taaggaaatg atgtcaaagg
    17221 cagtatcttt caaagtggca cttccttgtg gtttggtatc tttggtttct aaatctcgtt
    17281 tctgaatttt gacaccgcca cgaatgactt tatctgatac ggaaaactgg ttacttccag
    17341 ttaatacggc aaggtcgccg tcctcggtaa tctgcgtaag gtataaggct tttatctgtt
    17401 cttcactacc attcgcctgc atatatgcac cgtctagtaa gtagcccgtt ggcgatttcg
    17461 tttcttcgac cgttagtgta ccaagaggta aaaacgcttt tccgtcctgc atatagaagc
    17521 tatcgccaga taccttgtat gcgtctgcta attttgtgat gtaatgggtt gtcccgttac
    17581 tgtctgtttc agcggttgtc tttgtaaccc atgtacgagt agcttcggca ggaagattgt
    17641 ctttattgta gaagcctgca taatatttcc atgtaaactc cgccccctct aaagaagcgt
    17701 tcccttgtgg gttggctttc tgtgtttcca tgtcaatttt aaaaagctca atcaaagtat
    17761 cggtaacttt tggcgtatca gatactttca aagtcgctgt ctttccagct tcgactttta
    17821 aggaatacac cgtagtatca actttatacc ccaatggtgc agataattct ttgatatata
    17881 cggtagtagc tttgacttct gcaacctctg tatttccgct gtcgtcagtc gtaagggtag
    17941 caatctgttt ggtgcaatcc ttatcggaaa agacaccgta agtcgcacca gcgatagagt
    18001 aatttccgtt accgttagta atgctggtat tattggaagt cttttgaagt ttggcatttc
    18061 cgacatttaa cttcgcccag aattgcccta attcctgccc ctcgccagag tagatataac
    18121 cgccgcattc atagcgtcct ttgttttctt tcacaaaggc ttttgcacca gcaaacactt
    18181 catcttgtgt tgcctttggt atttcatcat aagaagctcg cacgttatca cattgccagc
    18241 ctaagtgaac actcaatctt tgccacacaa caagctgtct taaaaggtag gcgtgatttt
    18301 tgcttatccc attgtggttg tcggtgtatt gcttcacata ttcgatagat aaggcaacat
    18361 cacttatctg gtcggcactc atacgggagc ttgcgtcagc tctggtcttg tagccgttct
    18421 taaaatctgt gttaatatcg atacaataag cgtcctcgcc ctcaacggtc aaatgcccct
    18481 cgttaaaggt agaaccaatc gaaccgtcat tcattacttt ttcaacgata ccgacacgtt
    18541 cttcactttc cgtccagtat tgcttgcttt ctgcatggac aagtgtagtc ggtaaagcag
    18601 taacgacagt cgcaaaggca agtacgcccg tacacaatcg tttcaatatc tttttcataa
    18661 ttctaaatgc tcctttcatt ttgggtaata aaatagccgt ccataatgaa cggctggaaa
    18721 tagaaaagga acgtcaattt aggcgttcca tagtctatga aatattcaat ttttttcttg
    18781 cttcaatact gatgtgccgt accatatctt tgcatatcta ggaaaacttg cgtatcaagg
    18841 ctcattcatt ggtagtaaat tagtagtaaa ttgatgtgtt tgtttccttg aaaatgctga
    18901 tagatactat gttttagcgg ataatcaact ttttagtatg ttttttgcat aaaaattgag
    18961 tagatagtag ttttattgta cttaatgatt gtatttaagt gattagattt gaaattgaat
    19021 aatgtagata ataaaaaaat attaacagaa cttaataaac tccattaata tttttttatt
    19081 atttttgaaa ctgtgataat tatcacggaa gcatagaaaa taaagtgtta agatttaaac
    19141 aagatagtaa gtaagtaaaa ctaaaatata agaaaggtga taaaaatgaa actaaaatta
    19201 gattcatcta agttcaatga aggtctgaat tatctaaaaa aagactacaa gatatttgca
    19261 ccagttttaa taccatttaa aggtactttt tcagatactg atgttataag atacaaggag
    19321 gtaaacgctt ttgaagaaat ggaatttgct aagaaagcaa acttttcacc aaaagatgtt
    19381 gtacttccca taaatcaagt attgttttat tttactgaaa aagagtttaa ggaaagcgat
    19441 ttagacaaca agaaaatttt aattttctta agagcttgtg acataaatgg tataaaaaga
    19501 cttgatgaaa tatatctaaa taatggtgaa gaaaaagatt atttttataa aaatatcaga
    19561 gataaagtaa agtttgcact tgtagggtgt aaggaaagtt ttagaaactg tttttgtgtg
    19621 agcatggatt ctaacaaaac tgataattat tcaattggat taaatataga aggagataca
    19681 ctgtacttag atataaagga tagtgaattt gaagtattta atggagaagc aactgaattt
    19741 aatgttgatt atgtaactga taatttaata tctgtagatg tgccagagaa tattgattca
    19801 aatgaaatga taggaaattc aatatgggat gaatatgata caagatgtat aggatgtggt
    19861 agatgtaatt ttgtatgtcc aacatgttca tgttttacaa tgcaggatat tttctataag
    19921 gaaaacgaaa atgtaggtga gagaagaagg gtttgggctt cttgtcaagt agatggatat
    19981 acagatatag caggtggaaa ttcttttaga aaaaaacaag gcgaaagaat gagatttaaa
    20041 actatgcata aaatacatga ttttaaaaaa agattcggat atcatatgtg tgttggctgt
    20101 ggaagatgtg atgatgcctg tccgcaatac atatcatttt caaagtgtat agaaaaaata
    20161 aatgatttag taattagtaa ggaggaagta taaatgaacc catatatacc agtaagtgca
    20221 aaaatactag atatagttaa acatactgat aaagaatgga cttttagagt aaactgcaat
    20281 acaaaaggtg tattgcctgg aaagttttat gagatatcta tacctaaata cggtgaaagt
    20341 ccaatatcag tatcaggata tggagaggat tatattgact ttacaataag aaatgtagga
    20401 aaagtaacta gcgagttgtt taattataaa gaaggagatt ctttttttat aagaggtcct
    20461 tatggaaatg gatttgatgt atccctttat gaaggaagag aaattgtagt agtggcagga
    20521 ggaagtgcat tagcccctgt aagaggaata gttgaatatt tttataataa taaagaaaaa
    20581 tgcgaaaagt ttaaattaat agtaggattt aaatcaccaa aagatatttt atttgcagat
    20641 gatttaaaga gatggagtaa aaaacttgat attttagtta ctgtagatgg agcagaggaa
    20701 ggatatagtg gtaatgtagg attagtaaca aaatacatac cagaattgga tatgaaagat
    20761 ataaacaata cttctatagt tgtagttgga cctccaatga tgatgaaatt tacagtagaa
    20821 gaatttttaa aaagagattt agatgaaaag aacatttggg tttcttatga aagaaaaatg
    20881 tgttgtggaa taggtaaatg tggacattgt aaaatggatg atacttacat atgtttagat
    20941 ggaccagtat ttgattattc atatgctaaa aatcttattg attagggggt gaagtttgtg
    21001 ataagagatt taaataccaa aaaagtaatg aaaaatgctt atagaattac aaaaacaaaa
    21061 tatgagactt ctttaagagt tagagtacct ggaggtctta ttgaccctga gagtttagct
    21121 gtagtttcta aaattgcaag tgagtacggc aatggacagg tgcacataac tacaagacag
    21181 gggtttgaaa tattaggaat aaatatggaa gatatggaag aaataaataa aattatacaa
    21241 cctgttattg aaaaaatgaa tataaatcaa agtgagaaaa acgcgggtta tccagcagca
    21301 ggtacaagaa atatagcagc ttgtatagga aataaagtct gtccaaaggc tcaatataat
    21361 acaactgaat ttgctaaaag aatagaaaaa gctatatttc caaatgattt acattttaaa
    21421 gtggctttaa ctggttgtcc aaatgattgt ataaaagcta gaatgaatga ctttggaatt
    21481 attggtatgg ctttaccaat atatgaaaag gatagatgtg taaattgtgg agcatgtgta
    21541 aaaaaatgtt ctaagatatc tgttggagca ttaaaaacag aaaataataa ggtggtaaga
    21601 gataaagata agtgtatagg ctgtggagag tgtgtactaa attgcccaac aaatgcatgg
    21661 actagagatg agaaaaaata ctatagatta gctataatgg gtagaacagg aaagaaaaac
    21721 cctagactag cagaagattt tcttttatgg gtagatgagg acagcataat aaagataata
    21781 cttaatactt atagatatgt agaaaagtat atagataaag atgcaccagg aggaaaagaa
    21841 catataggat atataattga tagaacagga tttatggaat ttaaaaaatg ggcacttgaa
    21901 ggtgtagagc ttccagaaat aactaaaatg tacgaaaaca tatactggag tggaattaaa
    21961 tatctataaa ttgagtaaaa catatatttg aataaattaa aatttaatag aaggaagagg
    22021 gagttatgca taaagaaact ttggataaat taactaatgc agctataaat aagataaatt
    22081 tattaaacac aagtaaagta aaatatttgg tatcttctgc gtttgcagga ctttatgtag
    22141 gtataggtat acttttgata tttactattg gaggactttt gactgatgca ggttcaccta
    22201 tgactaagat agtaatgggg ttatcatttg caatagcact aagtttggtt ataatgacgg
    22261 gaacagagtt atttactgga aacaatatgg ttatgtctgc tggtatgtta aataaaggtg
    22321 taagtataaa agatacttct aaaatatggg catacagttg ggtaggtaat ttaatcggag
    22381 ctttggtttt aggcattata tttgtaggaa ctggtctagt agacaaagga cctgtagctg
    22441 aattttttgc aaatacagca gctagtaaag catccatgcc atttacagct cttttcttta
    22501 gagggatttt atgtaatatt ctagtatgtg tatcagtgtt gtgttcgttt agaactaata
    22561 gtgatactgc aaaaataatt atgatattct tatgtttatt tgcttttata acatcaggtt
    22621 ttgagcatag tgtagcaaat atgacaattt atagtgtttc attattttca ccaacaataa
    22681 gtacagttac tattggagga gctatttata atttagtagc tgtaacactt ggaaatattg
    22741 ttggaggagc tttatttatg ggtcttggaa cttatatatt aggtaaagaa aagcttaatt
    22801 aaatttattg tataaattat aactctcttg ataaatacat ttttagtatt gttgagagag
    22861 ttatttttat atttttgaaa aaatatttga aacaaaaaaa atatgggagc atacgaaaat
    22921 atatcggtta tatatataaa aaatacgttt atatatgaat tatatagttc taaatataaa
    22981 aaaagttaaa gtcctaccgt aaaaaagcgc attttgaata agataacaaa tatgataaaa
    23041 ttggtcttgt tgaaattttt aggggggata tgatgacaaa gaaactagta atatcaaaaa
    23101 caaagttgtt tatttttata atagcattta tttctatatt tatatattta tttggaagta
    23161 aaaatacact tataggtgta ggtatagtaa cagctatgct aacactcttg gaaagagatt
    23221 taactatttc tccaattaaa aatttactga agtatttagc aataaatata atcttaggaa
    23281 tactatcatt ttttgcagta caaaatatgt atttaggagt tttattaaat tttatagcat
    23341 tatttataat agggtatgta tttagttatg acttaaaaag agcagtatat gtaccctttg
    23401 gtctaatgta tatatttatg gtaagtatac ctgtaggaaa aagtgaattt cctatgagat
    23461 tgtcggcttt agctgttgga gcagttataa taatgattgc tcaatttgtt atgaatagaa
    23521 ataggatgaa aaatgtcgga gataaagaac tgatatctat atgtgatgaa ttattagaaa
    23581 aaattagcct tttaaaaaat actattaatg atgatagttc tataattaaa tctagtatga
    23641 gaaaaataga tagctgtaat tatagaataa attctatatc taaaaatttg aaaatggtta
    23701 tttttgataa tagaaaagat gacttttata tcagtattag aggtatagac ataatgaata
    23761 ttttattttc attagaaaga ataagtttaa tcctggaaag atacaaaaag gatagtaaag
    23821 aatttgaaga tgaagatata aaaaatattt tagaagaaag tagcataaat agtaaatcag
    23881 atgttttagt tgtagcaacg aaagaaataa actatattaa gatgtgttta gaaaataaag
    23941 ataccataac tgataaagag atattaggat ttagagatta tgttattgct caggatacta
    24001 aaaatataaa tttaaaagaa atttatagtg ttttagaaaa tttatatgag tttttagtag
    24061 attataaaaa agtaaaatca agagacgaaa agaaagctga aagaaaatca aagatacctc
    24121 atgaatttaa acgactatca atatataaga aaaactttaa tttaaattct ataagatttt
    24181 cttatgcagt aaagattgct ttagctacag ctgtagcggg ttttattatg gattatttcc
    24241 atttaagaga tggaagatgg ataatgttaa ctgtcttttc attaacacaa ccttacgcag
    24301 aaaattgtat acaaaggtct agaaaaagaa tagaaggtac atttatagga gctgtaatat
    24361 ttattgtatt attttcaata ataaaagata gtacattaag gtcgttaata gttcttttag
    24421 cagggtatat aaactcttat gtagttgatt atagaaaatt aatggtatgt gtaactgttt
    24481 cagctcttgg ttcagcagta gttatgggag acccaaatgt tttaacaata agcaggattt
    24541 catatgtagc attaggagca attattgcat taatagtaaa taagtttata ttaccatatg
    24601 atgctaaaac aggctatcaa catgttatag aaatgtataa aggaatagtt aaaaatataa
    24661 ttgatgaagt caataagtct atagaaaatg tagctgatgt ttactatata aaaaatttat
    24721 tattgatacc atcacttatt gaagatagac ttatgttgat aaatgctata tataaagata
    24781 aacatcaaga ggatttttta gaaaatcaga aattactaat aagtaatatg tataatctat
    24841 atataaatgt taataaaaat aaaataaaag atgaagatgt tgaaaaaata ttgagagata
    24901 ccaattatat ttcaaattac aatgtagata agtatgatga aggaagaagt gtaattttag
    24961 agagtattgt aaatactaaa agtctaggtg acaagataat atgtctaaac ttactgcaaa
    25021 ctctaaatgg tgtcaaagaa atgtatagaa taagtaatat aactaaagtt tcaataaaag
    25081 aagcagctta gatattaaat tatagaatag ccatatattt aaaataaagt atatggctat
    25141 ttgttttata aaatactaaa agaaaataat atataaaaaa caaaaaacat atatttatga
    25201 attttatatg aaaatagtta aaatattgat ttttttcatt ggttgaaaaa acaatttact
    25261 taggataaac tataatagta ttcaaatatt atgggggtta gatttatgag taaagatgta
    25321 aaggtaaagc gtaataagaa atatgataca tttagtataa ctatggaact tgagagacct
    25381 ttactttatg atgaggttga gaacaaagat gataaaaata ataatagaaa atttaacata
    25441 aagaataaga tatttaaggg aataggtata ctatttgtat tgattttgct tggctttttc
    25501 atatggataa ataaaacata tgaacctcaa aagctggcaa aagaagctct agtcagcaat
    25561 agtaaagttg aagtaactgt aaatgaaaac atttcattta caccaaaagg aagaaaagtt
    25621 tctaaaggtt taattttgta tccaggagct aaagtagaaa tagaatctta tgctccactt
    25681 gcaagaaaaa tagcagaaag tggatatgag gttgtaatcg taaagatgcc acttaatcta
    25741 gctatacttg gaactaataa ggcacagaaa gttatggatt cttacaacaa catagaacat
    25801 tgggtaatag gaggtcattc attaggagga gttgcagcat caaattttgc acgtgataat
    25861 aaattaattg aaggcgttgt atttttagct tcttatccaa tgggtgatga gttgaaagaa
    25921 ttaggaaaaa aagttatatc tatatggggg agcaaagatg gagtagtcaa ttttaaaagt
    25981 cttgtagaat caaaacaaaa acttcctgat gatacaactt atgttgagat tgaaggagga
    26041 aatcatgcac aatttggaga ttatggaaaa caaaagggag ataatgatgc tattataagt
    26101 caagaaaaac aacttaatat tactgccaat agtatcatta aatttttaaa gaatatatct
    26161 taaactaaaa tttaaaaaat ataattttat aagttattat ttaaagtaaa tattaataat
    26221 ataatagaat gaatatttta ttacaagggg gattttaata tggaatacga tatgccactt
    26281 tacagaccac caagtgaggc ttatagtctt ataatacaag tcacactagg atgttctcat
    26341 aacaaatgta cgttttgtag tatgtacaaa tctaaaaaat ttaaaataaa gccactagaa
    26401 gtaataaaaa atgaaattga tatatttaga agatattata agcatgtaga aagaatattc
    26461 ttagcagatg gagatgcatt gataatacca atggagaaat taagagatat tatcttatat
    26521 ataaaagaag tttttccaga gtgtgaaaga attactatgt acggctctcc taaatctata
    26581 gagaaaaaat ctgatgatga acttaaagaa ttaagaaaac ttggtgttaa aatgatttat
    26641 ctagggcttg aaagtggaag caatgaagta ttagaagata ttaagaaagg ttttagtagt
    26701 gaacaattaa taaaagttgg tagaaaagtt aaaaaaagtg ggattaaatt atcagccact
    26761 gtaatagcag gtcttggagg aactaaaaaa acacatcagc atgcagttga tacaggaaaa
    26821 atgttaggag caatttcacc agattatgtg ggtgtactta gtttaatggt agaacctaat
    26881 actgaacttt acgatttact taaggcagga gagtttacaa tactagagga taaggctgtg
    26941 ttacaagaaa ttaaagagat gataaagaat atagatgcta atgaaaaaat tgtatttaga
    27001 agtaatcatg catcaaatta tgctaattta aaaggtgtat tacctgatga taaacaacgt
    27061 ctaattgatg aaattgatta ctatctaagt aaccaaaagc taaaaagaga agaatataga
    27121 agactatagt ttttgatata tcaatctata ttgataggta aatactaaga ttatttagat
    27181 acaaaaaatc atgctacatt aatagttgct tactattgat gtagcatgat ttttttatta
    27241 aaaaacaaaa tgg
//
LOCUS       DAMJVI010000044        27123 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.046, whole genome shotgun sequence.
ACCESSION   DAMJVI010000044 DAMJVI010000000
VERSION     DAMJVI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 27123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27123
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.046"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(113..973)
                     /locus_tag="PC545_001965"
     CDS             complement(113..973)
                     /locus_tag="PC545_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HDF2456733.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCD
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(1290..2618)
                     /locus_tag="PC545_001966"
     CDS             complement(1290..2618)
                     /locus_tag="PC545_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HDF2456734.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKDPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLKSRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TQERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGKIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPKLN"
     gene            complement(2766..3095)
                     /locus_tag="PC545_001967"
     CDS             complement(2766..3095)
                     /locus_tag="PC545_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDF2456735.1"
                     /translation="MVPEMEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(3119..4408)
                     /locus_tag="PC545_001968"
     CDS             complement(3119..4408)
                     /locus_tag="PC545_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2456736.1"
                     /translation="MEALQNNLRRFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVVAQLVVCGQVVDGVLDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVSGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSFNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATTLDDDSFDF"
     gene            complement(4424..4750)
                     /locus_tag="PC545_001969"
     CDS             complement(4424..4750)
                     /locus_tag="PC545_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2456737.1"
                     /translation="MNILLVCANGASTGVLVEKMKSFCSEHEKLKTKTINIEATSFEN
                     LKSYIEANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(4885..5514)
                     /locus_tag="PC545_001970"
     CDS             complement(4885..5514)
                     /locus_tag="PC545_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456738.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAVIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEVMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEKYKQEEVIDTAFKFNHWQY"
     gene            complement(5730..8387)
                     /locus_tag="PC545_001971"
     CDS             complement(5730..8387)
                     /locus_tag="PC545_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2456739.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQSELLGIKKGTKVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAMLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERMELVKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLRYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHGIEYFVFTSLG
                     EEEVINYNDINIINAVNTNLNKSVEMNIADASLFNNVKFKSMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGKCSAISFDM
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVRVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKHYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMSRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVAVINEQRINIRGIDY
                     ISKKDIDKDENINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKTLDYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(8746..9690)
                     /locus_tag="PC545_001972"
     CDS             complement(8746..9690)
                     /locus_tag="PC545_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009527644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456740.1"
                     /translation="MNREKILEIKNLKQYFHLDKSTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIIRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGICKNKTDRMNKVYEMLKLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRNRIEYNSN
                     GYDCSNEKSLSWIEVSDGHFVYSSKSEINKYQQNLKVV"
     gene            complement(9683..10708)
                     /locus_tag="PC545_001973"
     CDS             complement(9683..10708)
                     /locus_tag="PC545_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456741.1"
                     /translation="MEHLLEVNNLSVSFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSDLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLNPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPEVDVPVRVNC
                     GRVISNE"
     gene            complement(10783..12351)
                     /locus_tag="PC545_001974"
     CDS             complement(10783..12351)
                     /locus_tag="PC545_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2456742.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADTPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGVLPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQITPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNPTIDKI
                     LQKARETEDKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGVFWNIADWTIE"
     gene            complement(12464..13375)
                     /locus_tag="PC545_001975"
     CDS             complement(12464..13375)
                     /locus_tag="PC545_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456743.1"
                     /translation="MKPQYSDFEIVGENYILVAEDEHIETKKTLSKKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASELVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIKNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(13359..14345)
                     /locus_tag="PC545_001976"
     CDS             complement(13359..14345)
                     /locus_tag="PC545_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456744.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYSQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRIICKLGTITNCIPSFWVALVLILIFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLILSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEATI"
     gene            14705..15106
                     /locus_tag="PC545_001977"
     CDS             14705..15106
                     /locus_tag="PC545_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2456745.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDENELSDLVKNLRNILAKVKER"
     gene            15109..15507
                     /locus_tag="PC545_001978"
     CDS             15109..15507
                     /locus_tag="PC545_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HDF2456746.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKDDEE
                     KKKFFHKFSIVVWTIWLVPYFIGMFIGMAG"
     gene            complement(15859..16083)
                     /locus_tag="PC545_001979"
     CDS             complement(15859..16083)
                     /locus_tag="PC545_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HDF2456747.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(16118..17485)
                     /locus_tag="PC545_001980"
     CDS             complement(16118..17485)
                     /locus_tag="PC545_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2456748.1"
                     /translation="MKEYIINNVEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGKAGH
                     GCGHNLLGVAGVGAVVSIKKLIEENKFNGTIKYFGCPAEEEGGGKTAMCINGCFDDVD
                     CAFTWHPFDINAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNVGANY
                     LREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVAQGAA
                     MMTETEMEYKVTDGIYDYIPNQCLTDLVYDNMVFVGCPKNTPEEEEFAKKLCDTLTRE
                     ERLGVATSLQNDKSIVESYIHKEIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMAAWPV
                     GIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSLDGQK
                     FKPLV"
     gene            complement(17569..19104)
                     /locus_tag="PC545_001981"
     CDS             complement(17569..19104)
                     /locus_tag="PC545_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456749.1"
                     /translation="MLNNLSNININENNLIKNQYSISLLKECLNCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYAIDAYINKLGDIEKRVVELTAKN
                     VKKVYLGGIEILEEELVRLKDFYKLMMSRKLDTNSIAYNDTLMELSSFFKSYIVKFEA
                     QDVPVSIDYPLALDDMDVKGIYYVKNYIESIDIENRFCNLFKKKDREKLFYDYGVTYK
                     IDCDAILVNLFELIINNCIFSTILGNEVTNLEISKYEYEFLEKQFKNMYNNITEDKVI
                     DSEESNIIELIEVDDSFNTEVDSNLDYNDFEEINREIRINKKITLLLMEAIEVLIKRL
                     SIEDEDVIRYIKKYEKIFIESVLSSIKSNTFKSMVTVSKNKKEHLEDYIVDDESKLDD
                     ESFREIFNEILDSTSIIEKIRIIKENINAKKDFIDILESECLFGEEYLMLFASLSELE
                     LAILGSVVFYEDIRMREINILEFMLNEKTETTLWKIEYIKFMKRQSEDKIYSIEEHMN
                     KIN"
     gene            complement(19127..19561)
                     /locus_tag="PC545_001982"
     CDS             complement(19127..19561)
                     /locus_tag="PC545_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456750.1"
                     /translation="MISPIILSSINQNLKEIERNELLETNIESGDYGLALSESDVKDI
                     INSRDNTLKGYGRIELDIKVTKQLIENIYTSQYTNVDNYLEAINDMQEIFYYLKNETD
                     DKICDDEIIEILGEFYEKFSGNMDNVRGEADEFAKKFKFGEV"
     gene            complement(19821..20732)
                     /locus_tag="PC545_001983"
     CDS             complement(19821..20732)
                     /locus_tag="PC545_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HDF2456751.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYKITEEDELYIVPTEDAVFTMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLEFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSTDEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLVFSKFYTEQLDWIVSFAKDMCKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            21028..21381
                     /locus_tag="PC545_001984"
     CDS             21028..21381
                     /locus_tag="PC545_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDF2456752.1"
                     /translation="MDRKRELKQLYKEMKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SLKFQLGAGSCMIKELNNEWKKYGEKAFTFEVLELIKHDDNKTEKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            complement(21600..22541)
                     /locus_tag="PC545_001985"
     CDS             complement(21600..22541)
                     /locus_tag="PC545_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HDF2456753.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIQGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEKMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLVNG
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNVIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVV
                     LVSNKSDKSILSGASKVTAIGISDKSIIEQCLDAVKK"
     gene            complement(22642..23877)
                     /locus_tag="PC545_001986"
     CDS             complement(22642..23877)
                     /locus_tag="PC545_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDF2456754.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSEVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINRLKYQFKVNI
                     DKAMNDFKPELIICHHLYLLTAFVREMVKDIKVMSICHGTCLRQLNTIDLEKEYIIAN
                     IRKLDLIFALHENQKYDIIKTFGVSQSKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSKDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELYDFENNLYNAIDSKIQSLLNNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLI"
     gene            complement(23921..24775)
                     /locus_tag="PC545_001987"
     CDS             complement(23921..24775)
                     /locus_tag="PC545_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456755.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWVLDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINTGIIEVKKEYELRLNGFKVEKLDL
                     PSSSIVVVKFHENKVEYFLLGDCALFIKNGDSKVIKDRSICKFDNTVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKEGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKVPRFKVKDDSSC
                     LYLDIYND"
     gene            complement(24903..26078)
                     /locus_tag="PC545_001988"
     CDS             complement(24903..26078)
                     /locus_tag="PC545_001988"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HDF2456756.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGVPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDSAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            complement(26177..27061)
                     /locus_tag="PC545_001989"
     CDS             complement(26177..27061)
                     /locus_tag="PC545_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDF2456757.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YEYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEHPLKLDISSV
                     VNGEVRQDSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DIVECKVEGIGVLKNIVD"
BASE COUNT         8653 a         4852 c         2790 g        10828 t
ORIGIN      
        1 tttattaaaa atacaaatta tattttatta atttttacta cttgtaaatt attatcttat
       61 acaatgaatt tgtattatat aataagtatt tttaggattc tgttacataa tattatttaa
      121 atattcttct atatttctct tcttcttctt cagtaaacac attaaataca acttttaaat
      181 ttttttcagg attttctttt agccatagtc taactctact tacagcaaga ttagctgcct
      241 ctttttttgg ataaccaaat acacctgtag aaattccaca aaaaacaata ttttttattt
      301 catcaatttc ttttattgta tttaaacatg atttataaca atgaagcagt tgcttttctt
      361 gctctttact tggctgacca ccagatacaa taggtccaac agtatgtact acaaattttg
      421 caggtaaaca ataacctcta gttatcttag catctccagt atattctaaa tgcccttgtt
      481 tttttattat tttgtcacaa tcttctctaa gtctaggacc tgcgcatgaa tgtatctcat
      541 tatccacgca tagatgtaat ggttgtaaac accctaataa cttgttattt gcagcattta
      601 ctattgcatc tgctcttaaa ttcgttatat tacctctcca tatggcaatc ccttcctcta
      661 cttcttctat atcatttaca tctactaact ctctttcttc acattcaatc aacaataaat
      721 tatgtaacat attaagtatc tctggagcaa tttcttttgg tggatataca ttcaaaagtc
      781 ctctaagtat atctctaggc ttaatttcat catttgataa attgtatatt ttagaaggaa
      841 cttcttttga aaaatatgca attaaagtgt ttatattttt tcttctttgt tcgtcagtta
      901 gaggcttgat agttttatca aaatcctcaa ataaatttac atcatcagca taatctctcc
      961 acttcatagc catctctcca ttcttgtatt ttagacttaa aaataattat gtatttatgt
     1021 acatatttat ttatttctat aaacacctac ataaatcctg ttaaaagtgt aaatttttta
     1081 ttatacaact tctctggtta tttctattga ttgatagtaa attattgtac ctaaatttaa
     1141 tccaactaac aagtaactaa acctactttc ctaactgcaa aatacactat gtttgccact
     1201 tgtatctcat ttgtagataa ataaagtccc tacaaataca ttaactaggc tatgtactaa
     1261 actgtaggaa ctttaaaaat atattattct taattaagtt ttggccaata atttttattt
     1321 gcttctatta aatcatctag taattctttt gcaactgttg cactaggaac tgtcttagat
     1381 agcataagtg cttgccacaa tttattataa gacttctcaa tccaagcatc aactactaat
     1441 ttttcaactg ctaattgttg ttctattaaa cctttttgga atgttggtat tttccctatt
     1501 gataatggtt catacccgtc ttttccaact atacatggaa tttcaaccat agcatcatct
     1561 tgtatatttt ctatagctcc attattttga actatcaata acatacgctc ctgtgtatta
     1621 aaagctagag ctgtagctaa atccactata aactctgcat gtattcctat cttaagccca
     1681 ctgcccttag aatgtccttg cttttctatc aatgcacatg aaccaaaaac ttctttttct
     1741 cttccatcaa taacttcatt ggcacgagta taatttacat cagaatgatt tacaacataa
     1801 tctggaaata gatagtactt taagtaacta cttggtattg tagttaaatc tgtttttact
     1861 atttctttag taaataaatt agtttcaaac caactatctt tcttgtcatt tttcattcct
     1921 tcttcaccat ttataaaacc atattctttt atatgttcta ctaattttgg taaaagttca
     1981 tttccttttt tatctttaat tgaagtatac caaccaaagt gatttaatcc aaagtatctt
     2041 atatccatat gtttacgaga tttgagtcct gcaatccttg ctatattatg ttccatacct
     2101 attggcatat cacaaatatt tattacccta gaattaggtc tcaaccttct aacagcttcc
     2161 gctattattg atgctggatt agaataattt aacatccaac aatttggaga atacttttcc
     2221 atataatcta tgttttcaag aacaccttct atagacctca ttccataagc tattccacct
     2281 ggtccacaag tttcttgacc aactacccca tgttttagag gtatcttttc atctagttct
     2341 ctcatttcaa gttttccaac tcttatatga gctaaacaaa aatctacatc tgtataagcc
     2401 tctttagggc atgttgttgc taaatactct atattagggt ccttttcctt tattagtatt
     2461 tcacaagctt tagctacctt attttgtctt tcttcatcat tatcatataa tttgattgct
     2521 cttaatggta acctatctaa gttatctagc aacattaata tgatttctgg ggtatatgta
     2581 cttcctcctc cagctattac tactgaaaat ttttccataa taatatctcc ttttctattt
     2641 aaaaatttac tgtatttata aataaattta taaatactag tactttaaag taaaagggag
     2701 ttcatttatt aaatctattg atttaataaa ctatagccaa acttcaagca ctatttacaa
     2761 gaaatttaat tcttattaat tcttgggtcc atcatctcaa atatatctac aagttcagat
     2821 actaaagaat gagcaaataa tgcatttgaa taatggtctt cagagtgcac aactaaaaaa
     2881 tcagtcccac tatcttcact attcatagca tttgatagta attctaaatg atgctttgat
     2941 gctgtttgta ttttctctga ggctgaattt aaaaggtgtt ttgctttatt gtagtcacct
     3001 ttttttattt ccttaagaga catcattaca tcacttttag catctcctgc ttctgatatt
     3061 atactaaata ctttttcttc catttcaggt accatatcct tctccttcta atgacttgtt
     3121 aaaaatcaaa gctatcatca tctaatgttg tagctaattc tgcattttta tttaattctt
     3181 ccttctccat tatcttaaag aatggataat atatcaaggt cataagtatt atacttgcaa
     3241 atactaatac gattgccttc caatctaatg tcatcaaaaa tgctgcaacc cctggaggta
     3301 aaaagcctgg tggttctgca ataggtaatc ctactaatcc tacttttaat gccataaaat
     3361 taagtgctcc aaccaataca ggtccaaata caaatggtac aaacatatat gggttcataa
     3421 ctattggtgt tccaaatatt accggctcat ttattccaaa tagtgatggt aaaatcacaa
     3481 cttttcccat actattaaaa ctcttacact tacttcttga cataaggtac actaatccca
     3541 aagttattcc tgaaccagtt acttgcaaga aaccatagta aaatcctatt gtaaacacat
     3601 attttgggtc tcctcctgct gctatcagtg ttgcattttc tgccatatat gtagctgcta
     3661 ttggcatcca tatacttgaa gttattgctg ctccatgaag cccaaagaac cataataatt
     3721 gagttataaa tagtattata gttactgcaa aaacattatc tacactgctt atagctggtg
     3781 ctaagaaatt cattataata gctggtagtg gttgacctga gacattttta gttattgctg
     3841 ctattgctgt aaataacatt atacatacta tcattggagt taaatttcta aaacttttat
     3901 ttactacact aggtacttct tttggaaatc taattacgag atttttatca ttcataaact
     3961 tagtaatatc aactactaaa agtgctacta atatacttac aaataaacct tgtgcatcta
     4021 aataacttgt atctaataca ccatctacta cttgtccaca tacaacaagt tgtgcaacaa
     4081 ctgctgtcat gcctatttct acatgattaa gattatattt tttagctagt gcctgagcta
     4141 taaaaaatgc tgaatatata gataataaac tcatagtaaa tttattaggg ataagtaatg
     4201 tatccatatt ttcagataat aatgtttttg cacttccttc aggtaacata ttgtttaatg
     4261 ccataaataa catacttaaa gaaccaacaa ttattattgg ggtaatgctt atcatacctt
     4321 cttttatagc ttgaagatgc ctttgttttt caatcttttg tgctattgga agaagaaatc
     4381 ttcttaaatt attttgaagt gcttccattt ttattccccc caattatcta tttttatata
     4441 attcgattgc cgacttcata acagaaggag catccatacg accataatgc ttagtatcta
     4501 ttaaaccaac tgctattttg taattcttac aagcttcaac tacatcatct tccttaaacc
     4561 taatttgtgg tgctacaagt actacatctg tatcattagc ttcaatgtat gattttaaat
     4621 tttcaaatga tgttgcctct atatttatag ttttagtttt taacttttca tgctcactac
     4681 aaaaactttt catcttttcc acaagtacac ctgttgaagc tccatttgca catactaata
     4741 aaatattcat aattaccctc ctacaatatc taacctcttt tattttctaa ttttttatca
     4801 gccaattctt actctaattc ctatcagttt ttactttaat tcttattatt ttattttaat
     4861 tcttattaat atttactcta attcttagta ctgccaatga ttaaacttga aagccgtatc
     4921 tataacttct tcttgcttat atttttctaa tatttttaag tatctttctt tataatcatt
     4981 atctacttct ttttctggta ttactatact agcttcattt aaaaagtgga atgaatccct
     5041 tcccatttct aatcctctat atgtatgctt gtcaagtgcc acgtccataa cttctggaac
     5101 tttacccatt gcaaatcctt ttataattat attttttaat aaatcacttg atctatcttt
     5161 ctcacatgca caaagatatc ttattgcatg tatgaaaaat aaaggtctgt ctccatctgc
     5221 atatgaatat tcctttctta cttgatttaa attatttatg attattacag ctgatggatc
     5281 ccccattcct atatcttcaa ctgatattgt aagtatcctt ctccatagct tatcttctaa
     5341 ttctggtgat gatatatata gttcataagc aaattcacaa gcttcttcca ccatatttct
     5401 tcttatagct ttttgaaatg acgatattat ttcgtctgca ctaaatccgt ttcttgtagt
     5461 cattcttgcc catgggtcat ttgccttttt atctcttaat attttatctt gcatattatt
     5521 tactcctttt tatttaaatt ttaattattt atattactca tttaataata taagcaagta
     5581 ttatgccagt ttttttagac actttaaaac actgttattt caatcatttt tatcatcaaa
     5641 acttagatac tttgtctaag tatatttata tgtgtctaat attaatatta ttttagacta
     5701 aaaaaaaatt accaaataag tttttatact cattggtaat ctttttcgat tatttctgca
     5761 tattctaata ttaataatat ttgatagcac tctttttcat taatttctat attatatttt
     5821 ttatctaatt gagatactat atcttttatt ttgcttagac tcttgtgata atcaagtgtt
     5881 ttttctttat caagcttttc aatttctcta gtatttccaa tcaacccatc taccatgaaa
     5941 gctaaatgca tcataagtcc aattacttta tcgtatttct tttctatatt aaatactaaa
     6001 aacaattcat cagacacata ttttatatct ttaaatattc tattataatt agagcttttt
     6061 aaggatgtct ttaaaccttc aactacatca tcataaccta tacttatctt tataatattt
     6121 tttaatttat tgatattttc atccttatct atatcttttt tgctaatata gtcaattcct
     6181 ctaatattaa ttctttgttc attgattact gcaacaatat tactactttc tttaagtttc
     6241 gtaatattta aacttaaatc tttttctgtt ttatatggga cagatataat atctattcca
     6301 tctaagtacc tactcatctt ttcttctaat atttttcttg ttttattatc tacaccttct
     6361 gaaacatgtg atgctataat aattgtttta gacagatttt ctttattttg aatttcatta
     6421 gttccaaaat agtgctttct cgtgttagtg atagagttgt atacgtcatt taaaggtaat
     6481 cccatattgg ccttctgaac tgcttctaac acagttaatg tcgtcaccat aggaattact
     6541 cttactccac actttttttc tttctttata atttcatcaa aggagtttaa tgaacccata
     6601 tctgtaaata gtaatacatc tttattgtct atattattaa ttaaattttt aaaatctaca
     6661 ataacttcat catagctttt atctaacttc atatcgaaag aaatagcact gcactttcca
     6721 ataatatcac caactgcttt taccatacta cttgctgtag tttctccatg catagcaact
     6781 ataatattaa tgttattcac tattttattt tgttcatctt gtttagcttg tactatgaat
     6841 aatgtaatat atgctacagt ttctatagat aaaaataaat caaattcatt ttcaattatt
     6901 ccaactattt ttaatgctgt tttaaattca cttctgttat ttcttctaat ttgattcaaa
     6961 tctatgttgt ttgtagagct acttttatcc aatacgcttt ttatataaat tgccattgac
     7021 ttaaatattg attcacttat gtctatttct tcatcttctt taataatttg tcttattgat
     7081 tcacacatgc ttttaaactt aacattatta aatagagaag catctgctat attcatttct
     7141 acagatttat taagattagt atttactgca ttaataatat ttatatcatt gtagttaata
     7201 acttcttctt ctcctaatga cgtaaataca aaatattcta ttccatgtgt tataacttca
     7261 tctgctttaa tagacttctc tctataatca tcactcactt catttataga tttatcaaaa
     7321 tattttttct ctatataaac tggttcttta ttatctcttt tatatcttag atatccattt
     7381 gcaacagcta attttatatc attttgaagt tgacctacat tatttttaca atcataaagt
     7441 aatagtgatt taattatttc tttatgtact tttattgaaa tagaaatact ttttgattct
     7501 tccttgaaaa aatttttgac taattccatt ctttcaatca ttgtcctatc tctcaatgat
     7561 ggtatttcta ttttcattgg tattcttcta ttgaatgtat ctaatagtgt tgatgtcgga
     7621 ttttctgtag tagcacatat tattaatacc ttagactctc tttctttcgt tgcatctcct
     7681 agtcttttaa atgttccctt atctataaaa gtaaacaaca tttcttgacc ttctgctggt
     7741 agtctatgaa tttcatctaa aaatagtatc cctccatttg caatttccaa aagacctttt
     7801 ctggaagatt ctgcacctgt ataagctcct tgttctaccc caaaaatatg tcccataagt
     7861 aattgtgcat tattagcata atcagcacaa ttaaatgtta caaaaggagc tttatttttt
     7921 ctaattccac tatctataga ataatcgtac atatatctag caatcatact ctttcctact
     7981 cctgtttctc catagataat agtatgaagt ccatttgtag gatagagcat tgcgaccttg
     8041 catttttcta ttataggtct taaactatgt tctttaccta ttagtgtttc aaaactatct
     8101 ttttttattt tgttttctac ttttgttcct ttttttatgc caagcaactc actttgtgta
     8161 tcaagcaatt cattcttgtt aaggttaaga tttgcaggga tataatacac aggtcttcct
     8221 tttatcttcg taaccatacc ctcattgaat aattggttta aatatgtact tatattgttt
     8281 cttgcaattc ctgtcttttc tgataattgg ttagctgtaa tccctttttc attttcccta
     8341 tatgatgatt ctaaaacttt cattacttta tcttttttat atatcattac aagctcccct
     8401 acattatagt attttattaa ctaaaacccc tatacacact ttatttttta ataaactctt
     8461 attctttaac taaggttata atttatcaaa ctttccttac tttaaattat agaaaattat
     8521 tgtagaaaaa ataagattat tgctcatttt ttctacatta ctatttttaa ttttatagta
     8581 ctttcttaac aacatacttt acttgttttt aattttatta tatttgaatt tattatattt
     8641 gaaaagtaga aataaaaaac cctattttgg agtagtttta aaatttctcc aaaaataggg
     8701 ttttagtttg tatttcaaat aaatacctgt tcaagtattt ttatactaaa caacttttaa
     8761 attttgttga tatttattta tttctgattt agaagaatat acaaaatgtc catctgaaac
     8821 ctcaatccaa cttaatgact tttcatttga acaatcatat ccattagaat tatactctat
     8881 tctatttctt gacttagcat atctagggtc aggaacagga attgcagaaa gtagtgattt
     8941 tgtatatgga tgaataggat tattatacaa ttcatttgaa tctgccaatt ctactaattt
     9001 tccattatac ataactccta tacggtcact aatatgtctc accatagata aatcatgagc
     9061 tataaataaa caagttaaat tcttttcttg ttgcaatttt ttaaataaat ttactatttg
     9121 tgcttgtata gacacatcca aagaagctat aggctcatca gctattataa attctgggtc
     9181 tactgaaaga gctcgtgcta tgccaatacg ctgtctttgt ccacctgaaa aatcagatgg
     9241 atacttattt gcataacttc tatcaagtcc tactagcttt agcatctcat atactttatt
     9301 cattctatct gttttatttt tacagatacc ttgaattttt agtggctctg aaattatatc
     9361 tgcaattgtc atacgtggat ttaatgatga agtagaatct tgaaaaataa tctgcatact
     9421 tttgtttaca tcttttttta taaatttgta cgttttttta tctgatatgc aatttccttt
     9481 gtaaattact tctccaccag tagattcatg tagtcttatt atagttttac cagttgtaga
     9541 ttttcctgaa cccgactctc ctaccaatcc aaatatctca cccttatata tatcaaaact
     9601 tatatcatca actgctttta cagtagtact tttgtccaaa tgaaaatatt gctttaagtt
     9661 ttttatttcc agtatttttt ctctattcat tagaaatcac ccttccacaa tttactctaa
     9721 ctggtacatc tacttcaggt gcatctgggt gcaataacca agttgctgca ctatgtgtat
     9781 cattaatttt aaacattggt ggctcttttt cataatctat cttaagtgca tttttattcc
     9841 ttattgcaaa agcatctcct tttggtgggt tcaataaatt tggaggcatt ccaggtatat
     9901 tatataaata gtcatcctgc gaatctaaag taggtaatga acccaacaac ccccaagtat
     9961 atggatgtct agggtcataa aatatatcct ctacagttcc tatttctaca atctttcctg
    10021 catacataac tgctattcta tcagctatag ttgcaactac tcctaaatca tgagttataa
    10081 atattattga caaacctatt ttatgttgta aatcctttat taaatcaatt atttgagctt
    10141 gtatagttac atctaaagca gttgtaggtt catcagcaat caatacatct ggattacaag
    10201 caagtgctat agctattact atacgttgtc tcattccacc tgaaaaatga tgtggaaatt
    10261 gtttaaatct tttctcagga ttatcaattc caaccaattc aataagctct atggctctct
    10321 tttttgcctc acttttactt ataccttgat gaatgattac agcttctgca atttgctttc
    10381 ctattgatat agttggattg agagatgtca tagggtcttg aaatatcatt gatatattct
    10441 ttcctcttat atcttccatc tctttttcag acttcttaac caaatcactt gatttaagta
    10501 aaacctctcc atttttaata tatccattgt atgggagtat cctcatcaaa ctcttacaca
    10561 gtacagattt cccacaacca gattctccaa ctatagccaa tgtctcacct ttttttagat
    10621 taaaagatac atttcttaca gcctgaactt ctccttcttc tactttaaaa cttacagata
    10681 gattattaac ctccaataaa tgttccaaaa accatcaacc cctatgctat tcaaacctta
    10741 gggatatatt tttatcccta aggtttctaa ttaatttcag tattattcaa ttgtccagtc
    10801 agctatgttc cagaatacac ctacaccatg atgacctaaa actgtatcag gtgttaatcc
    10861 ttttatgttt ggttttccaa catatattgc atctatataa gcaataaatg tataaggcat
    10921 atccttagtc atttctactt ggaattgttt atataacttt aatttttcat ctttatcttc
    10981 tgtttctctt gctttttgaa gtattttatc tatagtagga ttagagtaag cactgtaatt
    11041 tgcaccttta tctgttccaa atactttata tgtatggtca tctgggtcaa atggacttcc
    11101 ccaacctatt aaatgagcat cttgattagc ccaatctgtt tcagttacaa caacagcttt
    11161 agcatcaaca ccaatttctt ttaactgttg tgcacatatt ttagccatat caactctaac
    11221 ttggtcactc tcacctgctg ttatctcgaa agctaactta gtaccttctt tttcatagat
    11281 tccatcagaa cctaatttcc aacctagctt ttcaatttct tgtttagctt tttctggatt
    11341 atattcaaac ttttctatat caggattatt atatggtccc atttgtaaag gtgaatatgc
    11401 tggtacacca tgtcctaata atacactgtc aactattgct tttctatcta ttgcatagct
    11461 taaagcattt ggtaatcctt tagctttttt atctttaaag aatttagaat taaaattgta
    11521 taatatacct ctatagtcgg ctgttttcat tatatttact ttaaaattct tttcgtcttt
    11581 ttcaaagtta gacatatctt taggagttat ttgagctagg tctaattctc ctgattttaa
    11641 ttgcattgcc ttagccttat catctggaac aatcttaaag actactttat ctaatcctgg
    11701 ttcttttaca aaataatcac tattcttaac aagtgttata ctttgacctt tgtcccattt
    11761 ttcaagttta aatggtccag tacctattgg tttttgatta aattcatcag tagctatatc
    11821 cttaccctcc aaagcatgtt ttggtaatac tcctactgtt aaataatcaa gcattgctgt
    11881 atttggagct tttaaagtaa tttttatagt attatcatta accacatcaa tttttgttat
    11941 atcttcataa tttgatgcta tctcagaagc attatctgga ttcattatag tttctattgt
    12001 aaacttaaca tcatttgctg taaatttctc tccatcatgc cacttaacat catctcttaa
    12061 attaaaagta tatgtatttg ttgcctcatc aaatttccag tcctttgcta gacaaggaac
    12121 caccttatta ttttcatcat gagctgttaa tccattaaat attaacgaat ttatttctcc
    12181 atgttcatat agtgcaggat ttatacttgt atagtcatta cttccataaa caagcacctt
    12241 tccatcttta ggcgtatctg ccttcttatc tttgtctcct ccactagaac aacctgccat
    12301 tagactaagt atcattacta aacttaaaac ttttaacttc tttaatttca tgatgctccc
    12361 ccttttgctt tttataaaat atttttcaaa gctttgaccc catttattta aatagatatt
    12421 tcaaatacct cagtatttgc ttatttaata ctcaaatgct gatttataaa ttactagatt
    12481 ttttattatt attctttctt atataattac ctatatttgt tatacacacc aatgtggtaa
    12541 ccaaaaatat acccggaatc agtataatcc accatctatt tgaaagtaat gcttcctctg
    12601 acaaagataa catacttccc caagaaacaa tttctattgg taaaccaatt cctaaaaagc
    12661 taagtgtcga ttcagtacca atagctgcgc ctatgtttgt tacaaccatg aacattattg
    12721 atgccacaaa atttggaagt aaatgttgct ttaatacata gaagaatcct cctcccatgc
    12781 tcttagctgc tagtatgtat tcgctatttt ttatttgtct aacttctgtt ctcactatct
    12841 tagatatatt catccaactt gttatcccta ttactatgga catagatact ggatttgaat
    12901 ttccaagtat agcttgtaca aatattataa ttaatattga aggtatacta agtatgattt
    12961 ctgtaaacct catcattgca tcatctacga gttcagatgc cgaaccactt atactaccat
    13021 aaacaattcc tataacagtt gatattacag tggaaaatag tcctataaat aaagatattt
    13081 ttcctccata ccatatcata gaatatatat ctctacccat tgagtctgtt ccaaataaaa
    13141 aagttttatt tggagctaaa ttagaacttg ctaaatccat gtatgttggt tcatgtgtca
    13201 ttataaaaga tgagaataca cttccaatga ctatgattga taaaataata attgatatat
    13261 atggtaattc tttaattttt ttagataagg ttttctttgt ttctatatgt tcatcttctg
    13321 ctactaaaat ataattttct cctacaattt caaaatcact atattgtggc ttcattagta
    13381 tcacctctat catatttcat tctagggtct attttattgt ttataatttg agctaacata
    13441 ttcccaaata ctactaaagc ccctgttatt aagcataaaa ctaataacat attataatca
    13501 tgatactttg cactttcaaa acaaagtgtt cctaacccag gataagaaaa aactttttca
    13561 actacataag ttcctcctag tatatgaggt atcgaaatgg ccattatact aatatatgat
    13621 ggcattatat ttctcaggca atgcttaaaa acaatagttc tattatttaa accttttgct
    13681 ttacataaaa gcacataatc ttctcttatt tcctctagta atttatttct tatcatatat
    13741 gtgtaatacc atagatggct taatataagt actgtaagag gtaagattag atgtgatatc
    13801 ctacttaata tactggactc ttctcccatt gaataagcac cactacttgg aagtatactt
    13861 aagtttatac taaatataag tatgagtact aaggcaaccc aaaatgatgg aatacagttg
    13921 gttattgttc ctaatttgca tatgattcta tcaatgagct tatcttcatg taatgtacaa
    13981 aatattccta acaataaggc tagcacaaag gtaagtatat atcctgaacc acctaatatt
    14041 atagtgttta tatagacatc atttataaca gaagtaacat tttgtttata tttaaatgaa
    14101 attccaaatt ccccttttga tgcatttgaa atccatttta tatattgact gtatattggt
    14161 tcatccaaac caagtttttt catagcattt tctttttctt gtgtactcat tctttctact
    14221 ccatccccat agtaagagat taatgggtct cctggagcaa gtcttgccat gtaaaaaaca
    14281 ataaaagata ataaaaacat tactaaaata aactgcaaca actttttcag tatatccttt
    14341 aacaaacccc aacccccctt tgttgcaata aaaaaaacca cataaaagca aacgtaaagt
    14401 aacgttaaaa ccttcgtggt tccattatca ttaaacaaaa ttaaattttc tatatttatc
    14461 tatgagttaa ttttagcatc atttattaac tgtgtaaaca aactttttaa aaattgttaa
    14521 aatttcatca aattatttta tttataccta aaataaaatt tttatattct attcttcatt
    14581 attttctata aaattataaa attttaggtt gactatataa gaaaagtata ttatattagt
    14641 ttgtatgcaa accatttaag aggtgatttt attgaaaagt aattatgact gttttagaat
    14701 agccatgctc ttaaaagaac tttattctaa aactatgtac acagtagaag aaaattttaa
    14761 agaaaatgga ttaacacacc aacagattat agttataaaa ctgatagctc ataatcaaga
    14821 acttacaata tcacaactat gtgatgaaat gtctttagct aaaggtacgg tttcaggcat
    14881 aatcagcaga ttagaacaga ttggatatat agaaaaattc aagaaatcta atgataaaag
    14941 aaatacatac gttaagttta caacaacagg atttgaattt gccacaaatt ttaaaattaa
    15001 aatgcaagaa agttttgatg atatttttaa aaattgtgat gaaaacgaat tgagtgactt
    15061 agtaaaaaat cttagaaata tattggcaaa agtaaaggag agataattat gaattataaa
    15121 ttaatacttg caatagtatt tataacatca gctttaattt tctacactat aggtgtattt
    15181 ggagaaagaa aggcaaaaat tttaaaaaag aaacatgtaa ttatattttg gcttggtttt
    15241 atatttgata ctttaggaac atttacaatg agtaacatag ccaacagcca tacttttgaa
    15301 gtaaaatctg ctttatcaca aaaccttcat agtattacag gtcttcttgc aattgtgctt
    15361 atgctttttc atgcaagttg ggctactttt gtcctataca aagatgatga ggaaaagaaa
    15421 aaattctttc ataagtttag tatagttgta tggactatat ggttagttcc atattttata
    15481 ggtatgttta ttggtatggc tggatagtaa aataaaaagt gtgtaagaaa tactgtaggt
    15541 aaatttgtat aagacaaatt tatctacatc cttctttcac acttttacat atttatttat
    15601 aatactccat tatttctttc atgtttctta atacctatat agaatattta attatttcta
    15661 tagtatttta ttattttctc acactttttc atatatatat tgttttacta tttcttcata
    15721 tgtatatatg aaatattcca ttatttctat aaatgtttta ttatctttct catacttttt
    15781 catatatatt gcttcactat tttttttact tctttattaa atacttatgt ataacataca
    15841 tttaataaac gctaactatt aaacacctaa tacttgagtt actcctggaa cttcactcac
    15901 caaaacattt tcaataatag ctttgattgt catagtcgct cctggacacc cactacatgc
    15961 cccttgtagt ctaactaaaa ccacaccatt ttcatttaca tctataagct ctacatctcc
    16021 accatctctt tgtaatactg gttttatctt ttcctcaagc actttttcaa ctttttctct
    16081 catagctatt ccccccagaa atattttatt agcaaaatta taccaatggt ttaaattttt
    16141 gtccatctaa tgatttatca aattcaattt ttgcttcatc aataatttta gtatccataa
    16201 atacatcata agcactacaa gccagtactt tagctgaatt tatcatagca gaaaatccta
    16261 tatttgaacc tgctgatgaa catgactgcc atgtatgact tgcaattcca acaggccaag
    16321 ctgccattgc aaattgagcc actggtatta cataacttac atctccaaca tcagtagaac
    16381 cagctagtcc tttatctttg tcaccgtcaa ctatttcctt gtgtatatag ctttctacaa
    16441 tagatttatc attttgtaat gatgttgcta cacctaatct ttcttctcta gtaagagtat
    16501 cacacaactt ttttgcaaat tcttcttctt caggagtatt tttagggcaa ccaacaaaaa
    16561 ccatattgtc ataaactaaa tcagtcaaac attggtttgg tatatagtca tatattccat
    16621 ctgtaacctt atactccatt tcagtttctg tcatcattgc tgctccttga gctactttta
    16681 ttaatctatc aagaacgtgt tctgcatcct ttgctttttt acctctaata aagtaccaac
    16741 tttcagcaaa tccaggaact acattaggtc ttccaccacc attagttata acatagtgca
    16801 ttcttataga gtcaataaca tgttctctca aataattagc tccaacattc ataatttcaa
    16861 cagcatcaag tgcgcttcta ccattatgag gagcttgagc tgcatgggct gttatccctt
    16921 taaatttaaa ttttacagat aagtttgcca gacttccacc tctccaaggt gcatttatat
    16981 caaaaggatg ccatgtaaat gcacaatcta catcatcaaa acagccattt atacacatag
    17041 ctgtctttcc tccaccttct tcttcagcag gacaaccaaa atattttatt gttccattaa
    17101 atttattttc ttctattaat ttttttatag aaacaactgc tcctacacct gctacaccca
    17161 acaggttgtg cccacatcca tgtccagctt ttccttctac caatggcttt ctttctgcac
    17221 ttacacattg tgatagctct ggtaaagcat catattctcc aagtatgcca atcacaggtt
    17281 taccctctcc aaaacttgct ataaatccag taggtaaatt ttctacttct tcaattttaa
    17341 atccttttga ctttaggtat tctttttgaa tgctacttgc atacttttct tcaaaggcta
    17401 attctggatt ttcccatatt ttcttagata aatcaattaa ttcttctctt atttcttcaa
    17461 cattatttat gatatactct ttcataattt tcctccaaat atatgtattt ttgatatgct
    17521 ttacagatta tattatatac taacattcta tttattcaca attttttgtt aattaatttt
    17581 attcatatgc tcttctatgg agtatatctt atcttcactt tgcctcttca taaatttaat
    17641 gtactctatc ttccataaag tagtttcagt tttctcattt aacataaatt ctaatatatt
    17701 aatttctctc attctaatat cttcataaaa tactacacta cccaatattg ccaattctaa
    17761 ttcacttaat gatgcaaaca acattaaata ctcttctcca aataaacatt cagattcaag
    17821 aatatcgata aagtcttttt tagcattgat attttccttt attattctta ttttttcaat
    17881 tatagatgta ctatctaata tctcattgaa tatctctcta aacgattcat catccaattt
    17941 actttcatca tcaacaatat aatcttctaa gtgttccttt ttatttttac tcacagttac
    18001 catactttta aaagtgttac tttttattga gcttagcaca gattcaataa atatcttttc
    18061 atatttttta atatatctta ttacatcttc atcttctatg cttaatcttt ttattaatac
    18121 ttcaattgct tccataagta gtaaagttat ttttttattt attctaattt ctctgttaat
    18181 ttcttcaaaa tcattataat ccaaattact atctacttca gtgttaaaac tatcatctac
    18241 ttcaatcaat tcaattatat tactttcttc agagtctata actttatctt cagtaatatt
    18301 attgtacata tttttaaatt gtttttctaa aaattcatac tcatattttg aaatttctaa
    18361 attagtcact tcatttccta aaatagtaga aaaaatacaa ttatttataa taagttcaaa
    18421 taaatttaca agtatagcat cacaatctat tttataagtc accccatagt cataaaataa
    18481 tttttctcta tcttttttct taaataggtt acaaaatcta ttttctatat ctatactctc
    18541 aatataattc ttcacataat aaataccctt aacatccata tcatctagtg ccaatggata
    18601 atctatactt actggaacgt cctgagcttc aaattttaca atataacttt taaagaaact
    18661 tgataactcc atcaaggtat cattatatgc tattgaatta gtatccaatt ttctactcat
    18721 cattagttta taaaaatctt ttagcctaac taactcttcc tctaatattt caatgcctcc
    18781 aaggtacact tttttaacat tcttagcagt caactccaca actctttttt ctatatcccc
    18841 aagcttattt atatatgcat caatagcata ccatattgaa atcaacagtt tttcggcaac
    18901 ttctgttttt actgatgtgc tttgaccatt tgtatatttt ttaatcaaat ccataagtat
    18961 tagagatatt tcttgttgta tattatatac ttctctttca tctatcactt tacaatttag
    19021 acattcctta agtagagata tggaatattg gttctttatt aaattatttt cgtttatatt
    19081 aatattactt aaattattta acatacaaaa tctctcctat catatattaa acttctccaa
    19141 atttaaattt ctttgcaaat tcgtctgctt ctcctcttac attgtccata ttgcctgaaa
    19201 atttttcata aaattcaccc agtatttcta taatctcatc atcacatatc ttgtcatcag
    19261 tttcattttt taaataataa aaaatttctt gcatatcatt aatagcctct aaataattgt
    19321 ccacatttgt atattgtgat gtatatatat tttctattaa ctgttttgta acttttatat
    19381 ctaattctat tcttccatac ccctttaaag tattatctct cgaatttatt atatctttta
    19441 catcagattc agaaagagct agaccataat ctccagattc tatattagtt tcaagcagtt
    19501 catttctttc tatttccttt aaattctgat ttatagaact caaaataata ggtgatatca
    19561 tttttaaatt ctcctttttt attttttcca ttgacttttt ttgtttttta tgctatactt
    19621 aaattaattt atattatttt accaaccact tataatagta gtgttaaaag tggtttttgt
    19681 caactcaaaa ttactatctc aattaaactt ttcaattttc tcaacaaaaa aataccatca
    19741 tttagatata taactttcta taacaacttt taattcagta gttatttact aaaatctaaa
    19801 caatgatata tttattaatt ttatttatct tttttggctc tacttctact tttctttaca
    19861 tttgagcaat ttctacaaga aggtgaacag tattgagatt tagaatttgt aggaatataa
    19921 attttaccac agtacttgca tttatttaaa gaagaaggtt cacttgtaat tgcaaaggca
    19981 tagatagtct caatagctgt tttcaaagaa tcaaactccc atgcaataat tggtttatct
    20041 aattggctta tagaaaatcc tatcttttga ggattaaacc ctccagacat aatagtaaca
    20101 ctttcagtta atagattaga tgtattttta tatacacaaa tttgattaaa atgcttacac
    20161 atatctttag caaaacttac aatccaatct agttgttcag tgtagaactt tgaaaatact
    20221 aaatctaaaa tcaatggtct ctttccaaaa aactttggag attcttctct ctttgataca
    20281 tctatacccc ttctatactt tttaaaatac acatcttcat cacttgcaaa tggtataaac
    20341 atgtttacat attcatctgt actcaatata ttctcttttg taatataatt cttttcaatc
    20401 atcagtactc tttcttctcc aactatatct ctattatata cacttgaata tattaaaccc
    20461 aataatccat acttttttac aaattcaagt acttctttct ttagtttcat ttcttttgtc
    20521 tcttttgata tatcttgtaa tgcctcttgt cctatgcgaa ttatatctac tattatatca
    20581 tctgctacat caaatgggtt atacatggta aacacagcat cttctgttgg aactatatat
    20641 aattcatctt cttcagtaat tttatattca taatcactat acctaatcca atcacttgca
    20701 aaattcccta ttaattctat ttttttattc ataataatca ttcacacctt tcaatgtaat
    20761 atataatttc atattactat tacactttta gtttattcca tattttctac actgtcaact
    20821 attcttttta tatttttagc atattaaaat ttataatagc ctttataatg gatttctaga
    20881 gctaaatttc ttgtttattg atgactaatt cataattaaa tttataaaaa ctatttacat
    20941 ataaaattaa tctctttata attgatatgt aatatatttt tacacttaca tattacatat
    21001 caattataaa gagaggtatc taaaatcatg gatagaaaac gtgaattaaa acaattatac
    21061 aaagaaatga aatttgacac aggtgtattc ataataaaaa atgatattac taaaaagatt
    21121 taccttggaa aaagtaatga cataaaaagc aaatttaact ctctcaaatt tcagcttggt
    21181 gctggctcat gtatgataaa agaattaaat aatgaatgga aaaaatatgg tgaaaaagca
    21241 tttacatttg aagttttaga acttataaaa catgatgata acaaaacaga gaaagattat
    21301 ttagaagaac tagatatttt agagatggtt tggttagaaa aattaaagga agataatact
    21361 tatgaaattt attccatata aaaaatcata taatataaga aaaggagata gtatatcaaa
    21421 atagacattt agttcataag atactttttt atataaaata aagtatcttt tcataatttt
    21481 aacaatgaaa aagaggctta tctccatttg atagcctctt cccatatatt ttataccatt
    21541 ataaaatgaa caatctacta attttcaatg tgtatattca ttttactaat aacactccat
    21601 tacttcttta cagcatccaa acattgctct ataatactct tatcacttat acctattgct
    21661 gttactttac tagctccact taatatactc ttatcacttt tatttgaaac taaaacaaca
    21721 ggagcatttt ttgcaagtgg tgatgcaact aaagcataaa ctaaatcgtc tccacttgtt
    21781 atatagaact cttttgctcc attatagaac ttttcaataa catttttatt agtttgatat
    21841 ctatcaacac caccaattct agttgctcta tatctgctca ctatgtaatc acttaatgta
    21901 gactctcctc caatagcata tactggaaca ggagaaactc caaaccatgg gtctaagtct
    21961 gtgtcttctt ctgctgaagg tatatctttt gtcaatatta taggagctcc atctctataa
    22021 gcaacagatg caacacttac tgcatcagct tcatccttaa atccatttac taaaaacatc
    22081 ttatcactgt ttttaatttt ttctatttca cctgcaattt tataacttgt atctactcta
    22141 tctacaccat caattcttat aacttgcatt cttctcataa gtaacatttt ttctacatct
    22201 ttacttatag aattttctcc acctattaca tatatcttag taactctatt cagtctttga
    22261 agcgttgctt ctggtatatc atccttatta gttaataaaa tagctgcatt atttactcct
    22321 gcaagaccac ttgcagcaat cccatctacc attgtagaat ctgcattgat aagaactgct
    22381 gttgtaaaat cttgcttatc tgaaataagc ccagctgttt catatttatc tactccttga
    22441 atattttcaa tcttatctag tgcgtttatt gatgttggaa aactaacact taacagtaat
    22501 gctgaaatac ctaacgccaa taccttttta ctaatcctca taaattacct ccctaaaaca
    22561 aatatattta gtattagtat aatataaaat catttaaaaa tagtgtaaaa atagttacac
    22621 ttttttataa tttaaataat tttagattaa acacaattca tccattaaaa taacttcatt
    22681 tattctatat gctaagccat cccacgtttt ttttgacata tctacagatg ttttcttatt
    22741 tgagttattc aataaagatt gtatcttaga atctattgca ttatacaaat tattttcaaa
    22801 atcatataac tcttcatctt ttggaatacc ttctttctcc ataaaaggta gagaaacata
    22861 ttctatcttt ccagaattat taatctcact accaatccac tctttaacac caagaatatc
    22921 agtagtgatt acatctgtgc cacaagataa agcttctaaa actacaactg gtaatccctc
    22981 ataaaatgat ggtaacacaa atatttgtga cctgttgaat agctcagcta aatctctctg
    23041 ttctaacttt cctaggaaat tcattttaaa tggagactta cttgccagtt taactatctc
    23101 attatatgat tcgatatcgc ttccagtacc tgcaaaattg acttctatta aatctttact
    23161 atattttaat ttagatatag cttttataaa tggaatcagt ccctttgatt tacatatctt
    23221 acctgcaaat actattttta ttttatcatc ctttatttta taatttttat tataaaatat
    23281 atcatcatta taaccactac caattacaac tactttactt tgacttactc caaaagtctt
    23341 tataatatca tatttctgat tttcatgaag agcaaatatt aaatctaatt tccttatatt
    23401 tgctattatg tattctttct ctaaatcaat ggtatttaac tgtcttaaac aagttccatg
    23461 acaaatagac ataaccttta tatcttttac catttcccta acaaatgcag ttaaaaggta
    23521 caaatgatga catattataa gttctggttt aaaatcattc atagccttat ctatatttac
    23581 tttaaattga tatttcaatc tatttatcat gtctatattt aaatctttat atcttgtact
    23641 ttcatatggc atagaatcac tcatacctac aacaggaaaa tttaactccc cacaattata
    23701 ctttactgga taaaaactta cttcacttgg aaaacaattt acatcatcat ttacatctat
    23761 acctgcaatt acagcttgct tatgaccgat tttttcgaat ccttttatca taccagacaa
    23821 atatatacca ctacctgtac tatttggttt ttgtgttatt atatgaagaa ttttcacttt
    23881 tcacttgcct cctttctaaa attaaatagt tttatacagt ctaatcatta tatatatcta
    23941 aatataaaca agatgaatca tcttttactt taaatcttgg cactttaacc gctttatagt
    24001 cattatcttc aaaatccctt actttattat gtatatatcc tataccaaat ttttctgcta
    24061 tacgtatcaa ttcttcttct tttattagat tatatctatc acatgcactt gtaaaaccat
    24121 cactcgttag catcaattga aaatcttttt ttatttcaat gtatccatat atagaatttt
    24181 ccacagcttc ctcatcaaaa tccaatatcc aatagccctc ttttgtattt ttcttcaatc
    24241 tattgcttac tattgtatcc ataacactag ttctaatctc atcaaatgcc atctcttcca
    24301 agttagaaag tttactcatt tcatcaaata cagtattatc aaatttacaa atactcctat
    24361 cttttataac tttggaatct ccatttttaa taaacaaagc acaatctcct aataaaaaat
    24421 actctacttt attttcatga aattttacaa caactataga actagatggt aaatctaatt
    24481 tttcaacctt aaacccattt aatcttagtt catactcttt ttttacttct attattccag
    24541 tattaataat actctttaat gattcttctt tatttatatt tttatataaa tacctattcc
    24601 accactgaac ataccacctt gcgtcacttt cagttgacac taaatttttt ccattaagtc
    24661 ctgtagcacc atctaaaacc catgccccaa atggatttat accgactaca tcttcattaa
    24721 atttagctgt tccttgatta cataataggc tatagtttct tatatttaaa gccatatcaa
    24781 cctccaaatt gaatatctaa aaataaagtt ctatttaaaa ttatacagca aataaaaaaa
    24841 tctacaacaa aacttgttgt agatttttac ttaatctata ttatctttat actttcagta
    24901 ttttaatgat ggaataatct attcttagta aatgccatga ctatgttata cttattacaa
    24961 gttaatataa cattatcatc acgaatagaa ccacctggtt gtgctataaa acttacccca
    25021 cttctttttg cacgttctat attatctcca aatgggaaaa atgcatctga accaagtgct
    25081 actcctgtta aagttttaag ccatgctctt tttccttctc cagttagagg ctctggtttt
    25141 tcttcaaaaa agttttgcca tatgccatca gcaagcacat ccatataatc atcagaaaga
    25201 tatacatcaa tagtgttatc tctatctgga cgacctatat cctttttaaa ttttaagttt
    25261 aatactttag gatgttgacg aagataccaa gtatcagctt tgttacctgc aagtctagta
    25321 caatgaatac gtgattgttg acctgcacct acccctatta cttgtccatc tttagcataa
    25381 caaacagaat ttgattgtgt atattttaat gtaataagag ctataattaa atcacgtttt
    25441 gcactatctg taaaaatttt attatcagtt ggaatatctt ttaataggtc ttcatttatt
    25501 aatatttcat ttctaccttg ttcaaaagta attccataaa catcctttgt ttcaataggt
    25561 tcaggagtat aattttcatc tatttttaca atattatagt tgcctttacg ctttcctttt
    25621 aatatttcaa gggcttcatc tgtatatcct ggagctatta taccatcaga aacctctctt
    25681 gctataatag ttgcagtctc cttatcacat acatctgaaa gtgctataaa atcgccataa
    25741 gaagacattc tatctgctcc tcttgccatt gcataagcac aagctaatgg agaaagctct
    25801 aaatcatcaa caaaatatat ttgctttaaa gtatctgata aaggtacccc tactgctgct
    25861 cctgctggac ttacatgttt aaatgaagtt gctgctggaa gacctgtagc ttcttttaat
    25921 tccttcacaa gttgccaact attaaatgca tctaaaaaat ttatgtatcc aggttttcca
    25981 tttaatacct ctataggaag ttctccattc ttcatataaa tctttgatgg tttttgattt
    26041 ggattacagc catactttaa ttctagttct cttgccatat actcatccct ctctataata
    26101 aattaatcat aagtttgatt aatcctatat taattaaatt attcgtacta ataaaattat
    26161 atggcatatt tatccattaa tcaactatat tttttaaaac tcctattcct tcgactttac
    26221 attcaactat atcgccatgc tttaaatata cttgaggatt catccccata gcaacccctg
    26281 ctggagttcc agttgaaatt atatctcctg gctttagagt cattcctctt gataaatcat
    26341 ttattatagt aggtatatca aatataaagt actctgtatt agaatcttgt cttacttctc
    26401 catttactac actacttata tctaatttta aaggatgttc aaattcttct ttatgtacta
    26461 tacaaggtcc catagatgta tgtgtgtcta agctttttcc tctaaaccat tgaacatgtt
    26521 tatcttgaag tgctctttca cttatatcat ttactattgt atatccaaat atatattcat
    26581 aagcatcttc ttttgatata tttatacctt ctttacctat gattactgca agttctgatt
    26641 catagtccaa cttctctacc aaatctccat gtaagcttat cttatcatcc actccaatta
    26701 ttttgtctgc tctcttagaa aaataaattg gataatctgg cataactacg ttttttataa
    26761 ctccatctgg gatttcattg acatggtctt tatagtttag acctaaacat ataatatctt
    26821 ttttaggtct tacaattggg gaacataatt taacttcttc taacttataa cactttgcgt
    26881 tttcaaatga attgtttttt attttttcta aatctcctgt agatacgttt tcaataaaat
    26941 cattcatatc agtatataat ttacttaatc ctaaactatt aatttcatat atactatttg
    27001 tttctgaatt aaatacacct attttttctt cattgctact acaaaaagtc acaaatttca
    27061 tttttattcc tccattcaat aagttgatta atattattta atttttatta gtttatataa
    27121 att
//
LOCUS       DAMJVI010000045        26965 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.047, whole genome shotgun sequence.
ACCESSION   DAMJVI010000045 DAMJVI010000000
VERSION     DAMJVI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26965
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.047"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            175..570
                     /locus_tag="PC545_001990"
     CDS             175..570
                     /locus_tag="PC545_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456758.1"
                     /translation="MNILKMKKTGIFLGGVLFGTVGVKILSSDCVKKTCTRATAGALK
                     VKDSVLETTTKIQENVEDILAEAKEINEQEDLSTSKVNVVQDTLKCEQEVLKTETIKT
                     VEDTNSNSIKCEEIKDNVIEEKNSTCTTE"
     gene            613..2712
                     /locus_tag="PC545_001991"
     CDS             613..2712
                     /locus_tag="PC545_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDF2456759.1"
                     /translation="MNFYIKHEIRGRIRFDLQLGKLNNKQSDTLLYYLTSINGITKAK
                     VYDRTGDAVIYYNGEREYIIREITKFSFNNSKIENLVPEHTSRELNNYYRDKLANKLL
                     VKSISKIFFPKSLRFFLIGLHSIKYFKKGLSSLLNKKIEVSVLDATAIGISIFRKDIN
                     TAGSVMFLLGIGELLEEWTRKKSIDDLAQSMSLNIEKVWLRKGDTEILIPISEIKEGD
                     LVSVTMGNMIPLDGVIVSGEAMVNQASLTGESLAVNKKEGSYIYAGTVIEQGNIVMCV
                     KEKAGTTRFQKIVTMIEESEKLKSSVESKFEHLADTLVPYSFLGSILTYAITRNPIKS
                     LSILMVDFSCALKLSIPISVLSAMRECNNNNITVKGGKFLEGVACADTIVFDKTGTLT
                     KAQPTVSDIITFQNYNKEDMLRLAACLEEHFPHSIANAVVYEAEKQGLSHKEMHTEVE
                     YIIAHGISTKVDDKKVVIGSKHFVFEDEKCTIPDGEEDKYNNLSDEYSHLFMAISGKL
                     SAVICINDPLRKEAKYVISNLRECGIKKIVMMTGDSEKTAKSIASKVGVDEYYSEVLP
                     EDKANFVKSEKLKGRKVIMIGDGINDSPAISESDVGIAMSEGAEIAREISDITISADN
                     LNNLIVLKQISNKLMKRVDLSSKFIIGFNLGLILLGVGGFVRPSTSAFLHNASTVGIS
                     LNSMTNLLENTNTYNYH"
     gene            complement(2937..8147)
                     /locus_tag="PC545_001992"
     CDS             complement(2937..8147)
                     /locus_tag="PC545_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDF2456760.1"
                     /translation="MANKLYSEIVNLLEEGRDELRKYDLKEKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDEETGQIYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRHTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKTLIGNVTEEKIRNILEKAKEDLDSQEKDYDGCKELVYL
                     LNVALSDGVSIKLCNPLDKQPEKLLDEMKNLPETFEKTDLGLMLTASCKIEIQKAVDV
                     TYKDLEKLLVPYIESVKKGMEDSISILSSINSLKDKKIQLDKMYTDLCSRTNNMKEIM
                     EFVQELSYGDGVQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYREEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLTK
                     IISGYQLNILSSIYMYGDCELKIDCNMEKEVFSGKNIVLLTEKLHIPEGTYSINVSGK
                     DAEKLVGESSIYDGMNGRNGNNAGTSGDIILSFKEIEKRGELKLYVDGGRGGNGQDGK
                     KGDTGITGEAGANAKIGSKKESKEFANVKGTKYYVVKAEKGKQGGTGGTGGNGGNAGL
                     GSKSGQVYLLGKAPLLKSAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GTSPPMDNSDRNYYYFDRYVFNHALNQKQAADDGEKLNALYEEHKDWIISKREDSGSA
                     GAEGTAGHSGDALKEKELLKQEYSLSIDTFFYEFFERFGFSGIKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYSFEKKNKAVLSLDDMNTKKEFYKEA
                     KEIYEYLINKLMQSDRIESFVGKNQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKTLRELEQGKKDELKEGYFNTYKESILTQIESGNQQIEVLKNELENK
                     MLSVNAKMNLLIQEIIKMKEDEQEDIEELKKKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYKWTNRMVNFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEERRTKFDQIKEIIVKSIDDDNYADKADDIQRAKEGLSESE
                     YKEIYTKIQKKEIGMLEANYNNKQQKDKLAALEKSKQAIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLNVKSSFESKIEVVKEAIKDKENNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELSTKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSDSGNQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVINQAKEQNRQIREFLDQDAYQWQ
                     KTDNHILKVALTGSLDGKSRVGFENTYSLYDCNEEEVKKLLTGDEISFEVPLNKNFDA
                     IKYVTMYANIPNLRRNILEDSLKVKVHIHLYDSGYFSLYDDKKDVYYQYVFPQNYINI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKNAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DSLTNEQKKKLESYFDKPSKYAWSDSIDILKSLGYIDFKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVDLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIIG"
     gene            complement(8162..10657)
                     /locus_tag="PC545_001993"
     CDS             complement(8162..10657)
                     /locus_tag="PC545_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDF2456761.1"
                     /translation="MPENNNQLLEEITLLLNEGKVAINKAENQNLILILGGTGCGKST
                     AINYLAGCVMMEVEDEDTGISYIDCLDPVAEIGSGAVSQTLYPNIVNMRKPIEGMDAK
                     LCDTAGFSDNRGVAHDISAAVSLGEVFKNSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKGSITFFISKSFLGRTEKQIYNVIKRKYEQLCENLDSNIEEKAWLFE
                     ELLKDNGKNIHLCKPLASDEREGLLKQVLELDEVKDKTSAFQYPISADARLVLDTLIK
                     EIQNNIVSSLEDYVKAYTEDFINIIGSITMIQEIAKYDEVVKVFKNWLESTTSLTIER
                     FINVLAKQSGLFLQDSCMKLQNKIKGNLDIIDALSKYTEHGTGEILLPSVHMEFIIEK
                     ASKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNREKLMEVANKKDYVYSVSDFNSI
                     LTNVTVTQNEELEDSITRLSAYGEEGNEIIREFIGKYILNPFIVKTININDKKGIEIR
                     ALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKDTIYMDDNLGMNFASEKNIILASNNL
                     DVGSNVKINVSGKSGELVDNSMYGKNGEDGRDAGNIYLYIKEEIRNYRLTLIANGGNG
                     SKGIDGENGETGASGSNGADAGYTSYFHGQMFKGYFEKGTSGKKGEKGGAGGNAGRGG
                     NAGKKGNIYFISDSEEQKNRIINHCDITVKDGEAGPNGRPGLGGYGGSGGYHGYNTLS
                     FSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPAREYAEYGDRGDN
                     GGFNAPNTKLSTAVSKVEENLIKEKYQGYCSEI"
     gene            complement(11340..11627)
                     /locus_tag="PC545_001994"
     CDS             complement(11340..11627)
                     /locus_tag="PC545_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456762.1"
                     /translation="MRLNVTKQNTIEVENSRKEDVYFVIVDNVFFERQGDVFNSITNN
                     NLNNYDVKILPTDNVFLELKITSTKDLEPVFIVVEDRQYNHGSIMFKPVLV"
     gene            complement(11647..11922)
                     /locus_tag="PC545_001995"
     CDS             complement(11647..11922)
                     /locus_tag="PC545_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456763.1"
                     /translation="MSLFVGGWSKFRAVEEEDLKVFNEAVGMLEGVGYEPLIVSTQVV
                     AGTNFKFICNATSVTNPPQNYLAEIVVFKPLPCDEHNATITSINIIK"
     gene            complement(12559..12738)
                     /locus_tag="PC545_001996"
     CDS             complement(12559..12738)
                     /locus_tag="PC545_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456764.1"
                     /translation="MGKDRFEKVYSQGKINATEIWVDKETGVNYVFHYYGHAAGFTPL
                     LDREGKPVISPIVSK"
     gene            complement(13023..14498)
                     /locus_tag="PC545_001997"
     CDS             complement(13023..14498)
                     /locus_tag="PC545_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2456765.1"
                     /translation="MKRKPQYLIIFEHIIQQIYTGKITIGDRLDSIKSMSEEYMVSKN
                     TIKTVIKMLSEKGFIETKAGSRPVLIQSTSKQSIESDLLYEKILQISEIYKIFSLIFP
                     SIAMYNIKRFTSKDFEELNEIIDCMEKNSSNYLVFKEQRLKYINKLISKTNNFLIHHF
                     CEIIQHDVIISQLAYNFDNLDNLDNCCIDFFRNKYIEQVRKTYLYGLDGNLIEFKKLL
                     TYMYKACRESILKMVENRAPQSIDTSSFNSVTLEKCYLYDLIVSDVICQIFEGNIKIG
                     DCLPSITSIINRYNVSLPTVRSAYNELNELGIAKTINGKGTYITLFSEYDDDYINTEE
                     GAKRLSLLLDAMEFVTITLEDVVLLLGNKMDLIAVDKIETHLRYLQNNCEECKVYPDF
                     VLLCKIVDYTQIYVLQETFLHLKQYMIFGIYLERFFHEEYNEILKTRFDVCFEILRYL
                     KKGDVERFAESFSNIFKESLQYLKKVISSLNVDMRVKLDIL"
     gene            15567..16745
                     /locus_tag="PC545_001998"
     CDS             15567..16745
                     /locus_tag="PC545_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456766.1"
                     /translation="MKYHIFLIITTILIIELCNIYICKIASFRNKNITIHKNYKYIFL
                     IKPLTFLIIIFTKLPLPLYYILFYIVSHKLLLSRKSSTNNHFIPNMAVISFCILHLIT
                     VSTISLVKNTSPDIIFANMTYKLISILIASILDFILWYSMNKIKEHIYIITQNQIEFS
                     LFLKFIWFTLGYILFDSIQITFAPPSSLTSYFILGSNILLILQLIIFINHIYSIEKNI
                     YLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNNLLFSLI
                     YIDLDKLKTINDTYGHLAGDNYIINFSSIVIENLGIDDIFARIGGDEFIVIMPKSNEN
                     TTKEFIEKISKIMDNTISFSYGIVEVSSENKMTVEEIINIADIRMYTNKQNNHKRNNN
                     "
     gene            17095..17976
                     /locus_tag="PC545_001999"
     CDS             17095..17976
                     /locus_tag="PC545_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456767.1"
                     /translation="MPAYLFDSNSIANIYRVFPISFSFIVTGLYFSYKNNKNNKINFE
                     AVLEDKKNLIFLLSLMFGMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     RYSFRITQIKGYDIKNQIEYVESVKTEYEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     TSNHYKFTLCFTDLNSLKPVNDKFGHQFGDKYISTTAQIIKKLINVENSLLFRYGGDE
                     FLFLLQNTSCVEAEKIMININYELSKISNTDEYPYALSISYGLVEWDGISDIETLISK
                     ADSLMYENKLKNKSTLN"
     gene            18167..18334
                     /locus_tag="PC545_002000"
                     /pseudo
     CDS             18167..18334
                     /locus_tag="PC545_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(18594..21161)
                     /locus_tag="PC545_002001"
     CDS             complement(18594..21161)
                     /locus_tag="PC545_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2456768.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGELFHNGEMPQEVSDAIKMAY
                     AGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDKVIDSVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYALTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSNP
                     LLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIVLQSEKE
                     LWGISEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAHSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEVLIEQLDKNKSLSDMNGIIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     AQQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRAIKSGQMITVDGLAGTVTFDE"
     gene            complement(21164..21769)
                     /locus_tag="PC545_002002"
     CDS             complement(21164..21769)
                     /locus_tag="PC545_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456769.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDGEPSPQQCLRKIMLNHLRFP
                     EQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRPLDSSEKMLDETMNIFLHGIMNDGGE"
     gene            complement(22143..23603)
                     /locus_tag="PC545_002003"
     CDS             complement(22143..23603)
                     /locus_tag="PC545_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HDF2456770.1"
                     /translation="MFNELKTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEVIKNTKESIKVWAEMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     IKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVGKIKPLVEKLTIGNPMDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLITGNVRKNNLFYPTLLDNVNVDMKIAWEEPFGPVL
                     PIIRVKDINQAIEIANQSEYGLQSSVFTSDIDKAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVREL"
     gene            23957..24535
                     /locus_tag="PC545_002004"
     CDS             23957..24535
                     /locus_tag="PC545_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456771.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGSFYTFFDSKEKLFFEVLNGIQNRLISSVDEILSNMPPKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYSNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            24586..24819
                     /locus_tag="PC545_002005"
     CDS             24586..24819
                     /locus_tag="PC545_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456772.1"
                     /translation="MIELIQRLNDIIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKFLIIIAIISIIINILIILDR"
     gene            24828..26078
                     /locus_tag="PC545_002006"
     CDS             24828..26078
                     /locus_tag="PC545_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2456773.1"
                     /translation="MVKSSNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIVFSPIGGAIADIFNRRNLMVIFDFSSSLTVLILAVFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQNSNIIKTIFTDIKDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKHLKITNLHISFIICAVMFIPMAVSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYVITVTQLKTPNELLGKVMSILFAGTAIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLLIAFVSKITLRNENL"
     gene            26239..26808
                     /locus_tag="PC545_002007"
     CDS             26239..26808
                     /locus_tag="PC545_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDF2456774.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
BASE COUNT         9042 a         4609 c         3365 g         9949 t
ORIGIN      
        1 attatttcaa ctatataaac tacatatgta aaattcatgt aaatttacta atataaatca
       61 aataaaatta ctaaactctc taaactaatt ttgtaaaaca tactctattt ttaacatttg
      121 ttaccttata cataagtaca aacaaaatct aactaaatga aaggataatg taaaatgaat
      181 attttaaaaa tgaaaaaaac agggatattt ttaggaggag tactttttgg tactgtaggt
      241 gttaagatac tttcaagtga ttgtgttaaa aaaacttgca ctcgtgcaac agcaggagca
      301 ttgaaagtta aagattctgt tcttgaaaca actacgaaaa ttcaagaaaa tgtagaagat
      361 attttagctg aagcaaaaga aatcaacgag caagaagact tatctacatc taaggtaaac
      421 gttgtacaag atactcttaa atgcgaacaa gaagttctta aaactgaaac tataaaaact
      481 gtagaagata ctaattctaa tagcattaaa tgtgaagaaa tcaaagataa cgtaattgaa
      541 gaaaaaaata gtacttgtac aacagaatag atttaatgat tattatgatt aataatatca
      601 ggaggaagta aattgaattt ttatataaaa cacgaaataa gaggtcgtat acgctttgac
      661 ttacaacttg gcaaattaaa caacaagcag tccgatactc tcttatatta tttaacttct
      721 ataaatggta ttaccaaagc aaaagtttac gatagaactg gtgatgcagt catttattac
      781 aatggtgaaa gagaatacat aataagagaa atcactaaat tcagttttaa caatagcaaa
      841 atagaaaacc tagttcctga acataccagc agggaattaa acaattatta tagagacaaa
      901 ttagcaaata aactgctagt caaaagtatc tccaaaatat tttttccaaa gtccttaaga
      961 ttttttttaa ttggacttca ttctataaaa tattttaaaa aaggtctctc ttccctgcta
     1021 aataaaaaaa tagaagtttc tgttttagat gccactgcta taggtatttc tatttttaga
     1081 aaagatatca atacggctgg ttcagttatg tttcttcttg gtatcggtga actattagaa
     1141 gaatggacta gaaaaaaatc tatagacgac ttggctcaaa gtatgtcact aaacattgaa
     1201 aaagtatggt taagaaaagg tgacactgaa atcctaattc caatttcaga gataaaggaa
     1261 ggagatttag taagtgtaac tatgggaaat atgattccat tggatggagt tatagtatca
     1321 ggagaagcaa tggtcaatca ggcatcactt acaggtgaat cccttgcagt taacaaaaaa
     1381 gaaggatctt acatatatgc tggaactgta atagagcaag gcaatattgt tatgtgcgtc
     1441 aaggaaaaag ctggtactac tagattccag aaaatagtaa ctatgattga ggaatctgaa
     1501 aaactaaaat cttctgtaga gagtaaattt gaacatctag cagatacatt agttccatac
     1561 agctttttag gaagtatatt aacctatgca attactagaa accctataaa atcactctct
     1621 atattaatgg tagatttctc ttgtgcatta aaactctcta tccctatttc agtactatca
     1681 gccatgagag aatgtaacaa taacaacatt actgtaaaag gtggaaaatt tcttgaaggt
     1741 gttgcatgtg cagatactat tgtatttgat aagacaggca cattaacaaa agctcaacca
     1801 actgtttcag atataattac attccaaaat tataataagg aggatatgtt aagacttgca
     1861 gcttgcttag aagaacactt cccacactcc atagcaaatg cagtagttta tgaagctgaa
     1921 aaacaagggc ttagtcacaa agaaatgcat acagaagtag aatatattat tgctcatggc
     1981 atttcaacaa aagttgatga taaaaaggtt gtaataggta gcaaacattt tgtatttgaa
     2041 gatgagaagt gtactatccc agacggtgag gaggataagt acaataatct atcagatgag
     2101 tattcacacc tatttatggc aatatcaggt aaattatcag cagttatttg cataaatgac
     2161 cctttacgaa aagaagcaaa atatgtaatt tcaaatttaa gagaatgcgg aataaaaaag
     2221 attgtgatga tgactggaga tagcgaaaaa acagctaaat ctatagcaag taaagttggt
     2281 gttgatgaat attattcaga agtcttacca gaagacaaag ccaattttgt taaaagtgaa
     2341 aaactcaaag gtcgcaaagt aattatgatt ggtgatggaa tcaatgattc tcctgctatt
     2401 tcagaatcag atgtcggaat tgcaatgagt gaaggtgctg aaatagcaag agagatttca
     2461 gatattacca tatcagcaga taatttgaat aatttaatcg ttttaaagca gattagtaat
     2521 aagcttatga aaagagttga tttaagttct aagtttatta tcggctttaa tttaggtttg
     2581 attttacttg gtgtaggtgg atttgttaga ccaagtacat ctgccttttt acacaacgca
     2641 tctactgtcg gaataagttt aaacagtatg acaaatttat tggaaaacac caatacttat
     2701 aactaccatt aatctccatt acataaaaca tatgatataa aaaatttata attcatcttt
     2761 ttaaaaaaat atgtcttaaa attagctttt ttctaatcaa aattatataa agcatacttt
     2821 acaaatttta aactcaactt atttctattt tttacaaaaa aggagatgat tcaaattttc
     2881 attttgaaac atcccctttt gtaaatttct ttaaaattta tatattaatt atattttcaa
     2941 ccaataattg tattttcaac caatctttct tcatagcatt ttaaatcatt ttgctttaga
     3001 taattaaatt cagactctac ataaatacca cagcctacca attcaattgt caaattaggt
     3061 atattttcct ttagtatact agtctgctca taaataattc tttccaataa atcaacttgt
     3121 aatatagaaa aatgcttcat aatctcccca tcactatcta tatctttctt gatttcatac
     3181 caactctttg acaaaattcc cttaaaatca atatacccta gactcttcaa tatatctata
     3241 ctatcgctcc atgcatactt actaggttta tcaaagtatg actctagttt cttcttctgc
     3301 tcatttgtaa gtgaatcata agaaagcttc ttattaaaat gtgccttcaa agaaatctta
     3361 gccatagaat atggacttct accagagttt aatcctgcat ttttgaactt ttcaattcct
     3421 ttatcttcta ctgaagacca tattgagttt attgaatctg aatcattgta tttaacatca
     3481 agaccatgtg taatatttat atagttttgt ggaaatacat attgataata gacatctttt
     3541 ttatcatcat atagagagaa gtatccacta tcatataaat gaatatgcac ctttactttt
     3601 aaactatcct ctaaaatatt tcgtctaagg ttcgggatat ttgcatacat ggttacatat
     3661 tttatggcat caaagttttt atttaatgga acttcaaaac taatttcatc tcctgtcaaa
     3721 agctttttca cttcttcttc attacaatca tatagagaat atgtattttc aaatcccaca
     3781 cgtgatttcc catctaaact gcctgttaat gctaccttca aaatatggtt atctgttttt
     3841 tgccattggt aagcatcttg gtcaagaaac tctctaatct gtctattttg ctctttcgcc
     3901 tgatttataa cctcgttttc atcttctgta atccctacat ggcatctatt taagatattt
     3961 actatattgc ttccaaatgg gaatgtccat agttgaaaag ctgctgcctc aacctgacac
     4021 aagtatctaa cctgatttaa ttcaatttga tggataagtt ttttctgata ttcattcatc
     4081 tgattaccac tatctgacat ttgataaatc aattctccca ttgcaagatt atcttttttc
     4141 ttatcaatct tatcagtaat gataatttga gtttccatca tatcctgtac ttttttaaat
     4201 attatagaaa tctcctctga attttccatt tttaagcttc caaactttgt gcttaattca
     4261 gttaaaactt ccttcatctt taatttactt atctcatttt caaaaatatc ttgattttta
     4321 actgtactat cgaatttttc tacaaatggt ataacagatt ctttcaaagt tttatcttta
     4381 aattccacca tttgagtatt caattcatct aatttcttaa tattattttc tttatcttta
     4441 attgcctctt taactacctc tattttactc tcaaaactag attttacatt aagacaatta
     4501 ttaacaccac taatcgcagt atttataaga gaacaagcag aacttccaat actcaatgca
     4561 tttttaattt tagatgtctt ttgctgtctt tcaatagctt gtttactttt ctctagtgca
     4621 gctaacttat ctttttgctg tttattattg taatttgcct ccaacatacc tatttctttt
     4681 ttctggattt ttgtgtaaat ttccttatac tcgctttcac ttaacccctc ttttgctctt
     4741 tgaatatcgt cagctttgtc tgcataatta tcatcatcta tagacttaac tataatctcc
     4801 ttaatttggt caaatttagt tcttctttct tcatcaacac ctgccaaatt ataagtcatt
     4861 ttatctaaag cattttttct atcttcactt agctgttctc caagtacaaa tctcatacca
     4921 tcagaatcca atttaaaatc tgaaaaatta accatcctgt tagtccattt gtattcctct
     4981 tctttagctg aaagattttc cttcaaagat tttgcactgt caaatgcccc tagacaagct
     5041 tgaccaacac cactaaatgc agtcagtaaa ctcaatgcgc tttgtattcc agaaagaatt
     5101 aactgtgttt tcatttgttc ctcaagtttt gcctgtttct tttttagttc ctctatgtct
     5161 tcttgttcat cttctttcat ctttataatt tcttgaatca acaggttcat ttttgcatta
     5221 acagaaagca ttttattctc aagttcattt tttaaaacct ctatttgttg atttccagat
     5281 tcaatttgag ttaggatact ttccttatat gtattgaaat atccttcttt taactcatct
     5341 ttcttccctt gttccaattc tcttaaggtc ttatccagat aatctgtcaa agaacgaata
     5401 tctgtaacac gatttcctcc aatttcactt tcttcaatca tctgcacata aaatgctgtc
     5461 ttctgctcaa gtgcaaataa aatcatactc cattcgctaa aattctgatt ttttcctaca
     5521 aaagattcta ttctatcaga ctgcatcaat ttatttataa ggtattcata gatttccttt
     5581 gcttctttat aaaattcttt ctttgtattc atatcatcca gagaaagtac tgctttattt
     5641 ttcttctcaa aactatagta cttctgaagt atttctctat ttaattgcat ctctattttc
     5701 tcaactgcat gaaaaatttc ataaatatgt gaaagttcta aaatatcagc ttctaaaatt
     5761 cctactgcac acaacagtct aagatttcta taacctttaa ttccagaaaa accaaatctc
     5821 tcgaaaaatt catagaagaa tgtatctatt gacaaagaat actcctgctt taacaattcc
     5881 ttttctttta gtgcatctcc agagtgaccg gctgttccct ctgctccggc tgaacctgaa
     5941 tcttcacgtt tgctaattat ccagtcctta tgttcttcat aaagagcatt taatttctct
     6001 ccatcatctg ctgcttgctt ctgatttaaa gcatgattaa acacatatct atcaaaataa
     6061 tagtagtttc tatcactatt atccattgga ggagaggtac ctatatatct tattgtttct
     6121 tcatatatac ctctaattcc accaagacct cctgcaccac cagctcctcc agagcctcct
     6181 tttcctgcta aaccatgatt tccatctttt ttacttatag cactttttaa taaaggagct
     6241 tttccaagga gataaacctg tccagacttg cttccaagac ctgcatttcc accattacct
     6301 cctgtgcctc cagttccacc ttgttttcct ttttctgctt ttaccacata gtattttgta
     6361 cctttaacat tagcaaattc ttttgactct tttttagaac ctattttagc atttgcccca
     6421 gcttctccag ttatccctgt atctcctttt tttccatctt gtccatttcc accacgtcca
     6481 ccatctacgt aaagtttaag ttctcctctt ttttctattt ctttaaaaga taatatgata
     6541 tcacctgaag tacctgcatt attgccattt cttccattca ttccgtcgta aatagaactt
     6601 tctccaacca atttttctgc atccttacca gatacgttga ttgaataagt cccttctggt
     6661 atatgtaatt tttctgtaag tagtacaata ttcttacctg agaagacttc tttctccata
     6721 ttacagtcaa ttttcaactc acaatctcca tacatatata tagatgacaa aatattaagt
     6781 tgataaccag atattatttt agtcaaatcc gataaattca ctttaggtat aggaatatat
     6841 atagaaagta cattttcttt ctcatatgat tttatatatg tacaactttg aatcttctct
     6901 atcctatact ctgcatcttc aagctttttg gcttcttctt ccctatattc ttcttctgct
     6961 atccaatatt tgatatcttt taatgtatct tctaaagtat tttttagcaa tgattttaac
     7021 aactcactta gaccaacaat atgagtctca ctacagtaac tatttaactc taaaagaaga
     7081 cgtgtttctt ctaatatatt cactttctgt acgccatcac catatgaaag ttcttgtaca
     7141 aactccataa tctctttcat attattagtc ctactacata aatctgtata catcttatct
     7201 aattgaatct ttttatcttt tagactattt atagaagata gaatggaaat agaatcttcc
     7261 ataccttttt taacactttc tatataagga actaaaagtt tttctaggtc tttataggtt
     7321 acatcaacag ctttttgtat ttctatttta caagaagcag taagcatcaa ccccaaatct
     7381 gttttttcaa atgtttcagg tagatttttc atttcatcca acaacttttc tggctgttta
     7441 tctagtggat tacataattt aatagataca ccatctgata atgctacatt taataagtac
     7501 actaattcct tacaaccgtc gtaatctttc tcttgactat ctaggtcttc ttttgctttt
     7561 tccaatatat ttcttatttt ttcttctgtt acattaccaa ttagtgtttt aggtatttta
     7621 ttgataagaa agaagaaatt ttctttatac tttgcataat caccaagcat atctttcaaa
     7681 tttttgcctg tttctatcaa taatgtatgt ctagagtcca gtagggattg atagtctaaa
     7741 ataactacca tcccttttac ctttgaagat gtggaaatag tttcccttac aagcatagca
     7801 ccagcaatca tatactctgc tccacggtta tcaaaaaaac ctgggcaatc acataatgaa
     7861 aaatctctat ttgcaatatc tacaacttct ggacataaag tcttagaata tacaccattt
     7921 ccaatttcga cagctgaatc ttttgcagtt atatatattt gtccagtctc ttcatccgtt
     7981 ttttcttcca tcacacagcc ttttaaataa tttatacaag tactctttcc aacgcctgaa
     8041 gcacctaaga ataacaaaat tgacttttct tttaagtcat attttctaag ctcgtctctt
     8101 ccttcttcca aaaggtttac gatttcacta taaagtttat ttgccataca tccatccctt
     8161 tctatatttc ggaacaatag ccttgatatt tttctttaat aagattctct tctacctttg
     8221 aaacagctgt tgataactta gtgtttggag cattaaatcc tccattgtct cctctatctc
     8281 catattcagc gtattctctt gctggtcctg ttttaccttc attccagctt ccaccactaa
     8341 attctatatg atatttaatt atactatcta acttataatc ccaacttgta aaatatcctg
     8401 tatagtatac agtcttatct ataccacttg gagaaaatga aagtgtatta tatccgtgat
     8461 agccaccact tcctccatat ccacctaaac ctggtctacc atttggacct gcttctccat
     8521 ctttcactgt tatatcacaa tgattaatta ttctattttt ttgttcctct gaatctgaaa
     8581 tgaagtaaat attacctttc ttaccagcat ttccacctct tccagcattt ccaccagctc
     8641 cacctttttc accttttttg ccacttgtac ctttttcaaa atacccttta aacatctgtc
     8701 cgtggaaata acttgtatat cctgcatcag ctccattact tccagaagct cctgtttcac
     8761 cattttcccc atctattcct ttagaaccat ttcctccatt tgcaatcagt gttagacgat
     8821 aatttctaat ttcttcttta atatataagt aaatattacc tgcatctcta ccatcttcac
     8881 catttttccc atacatagaa ttatctacaa gttctccgct ctttccactt acattaattt
     8941 ttacattact acctacatca agattgttag aagcaagaat aatatttttc tctgatgcaa
     9001 agttcattcc aaggttatca tccatataaa tagtatcttt agcaaagaac ataaatgtag
     9061 aaacattgtc atctactttt ctatttttga aatattccaa tacactgcta attactaagt
     9121 ttggaattaa tgctcttatt tcaatacctt tcttatcatt aatattaatt gtttttacta
     9181 taaatggatt taaaatgtat ttaccaataa attctctaat tatctcatta ccttcttctc
     9241 catatgcact taatcttgtt atactgtctt ctaactcttc attttgtgta actgtaacat
     9301 ttgtcaaaat tgaattaaaa tcacttacac tatatacata atccttttta tttgcaactt
     9361 ccattaattt ttctctattt ggtaatgtaa atacctgttt ttgcacttca taagaaacta
     9421 aaactttttc taaattcttt cttattataa tgaagttttg ttccattatc ttcttacttg
     9481 ccttttctat aataaattcc atatgtacag aaggtaataa aatttcccca gttccatgtt
     9541 ctgtatattt acttaaagca tcaattatat ctaagtttcc ttttatctta ttttgtaatt
     9601 tcatgcaact atcttgtaag aataatccac tttgtttggc taatacgttg ataaatctct
     9661 ctatagtaag acttgttgta ctttccaacc agtttttaaa tacttttaca acctcatcat
     9721 attttgctat ttcttgaatc attgttatac ttccaataat attgataaaa tcttctgtat
     9781 acgccttaac ataatcttct aggctagata cgatattgtt ttgaatttct tttatcaacg
     9841 tatctaaaac taatcttgca tctgcactaa ttggatattg gaatgctgat gtcttatctt
     9901 taacttcatc taattctagt acttgcttta ataagccttc cctctcatct gatgcaagtg
     9961 gcttacaaag atgaatattt tttccattat cttttagcaa ttcttcaaat aaccaagctt
    10021 tttcctctat attactgtct aaattttcgc ataattgttc atattttctt tttataacat
    10081 tatatatttg tttttctgtt cttcctaaga aactttttga aatgaaaaaa gttatactac
    10141 ccttaaaatt ttctttatga agacacatat ttaactgttg gaacaggtct agtacttttg
    10201 taagtctaga atctaatata tctgcttctg gtatcaatac aattactgca catatagatg
    10261 cagaattttt aaatacttca cctaatgaaa ctgctgcact aatatcatgg gctactcccc
    10321 tattgtcgct aaaacctgca gtatcacata attttgcatc cattccttct attggttttc
    10381 tcatatttac aatgttcggg tataaagttt gagaaacagc tcctgaacca atctctgcca
    10441 cagggtctaa gcaatcaata tatgatatac cagtatcttc atcttctact tccatcatta
    10501 cacatcctgc aagatagtta attgcagtac ttttgccaca tcctgtccca ccaagaatta
    10561 gaattaaatt ttgattttca gccttattta ttgctacttt tccttcattt aataagagtg
    10621 ttatttcttc taaaagttga ttgttatttt ctggcattta ttttccccct tggttatata
    10681 ttttatttaa ataaagttta atcccttaag tagattgcaa agtttttata ctatatttaa
    10741 atttgttttt tattttttat ttttaataaa tttgcatttt cactaaattt gtattttcac
    10801 tcaataatta ttcttctttg tttattaaaa taactgtgtt gtataatata atctttatca
    10861 acacagttat tttatttaat gcgcttattt tattgtattt tatgtaatac agttgttttt
    10921 tatattatat gtccattatt ttcctgtggt tattacatat agatttttaa ttatatttgt
    10981 accattaatt taaacattcc tatactactg cttaagtttt acacctatac cagcttgaat
    11041 attttatttt tatttgctta aatagttcaa ctatactggt ttaaataggt tatctttatt
    11101 tgcttaaata gttcaactat actggtttaa ataggttatc tttatttgct taaatagttc
    11161 aactatactg gtttaaatag gttatcttta tttgcttaaa taagttcaac tatactggtt
    11221 taaataggtt atctttattt gcttaaatta gttcaactat actggtttaa atagttttcc
    11281 tttagtttaa ataattcacc tatacctact tgaatagttc acttttattt gcttaaattc
    11341 tataccaata ctggcttaaa cattatacta ccatgattgt attgtctatc ttcaactaca
    11401 ataaaaacag gttctaaatc tttagtagat gttattttta attctaaaaa aacattatca
    11461 gttggtaata tttttacatc ataattattt aaattattat tcgtgatact attaaataca
    11521 tcaccttgac gttcaaaaaa aacattatca actattacaa aataaacatc ttcttttcta
    11581 ctattttcaa cctctatagt attttgttta gttacattta gtctcatctt ccacctccta
    11641 ttaatattac ttaattatat taatacttgt aattgttgca ttatgttcat cacaaggaag
    11701 aggtttaaaa acaactatct ctgctaaata attctgtgga ggatttgtaa ctgaagtagc
    11761 attacaaata aatttaaaat ttgttcctgc tacaacttga gtagaaacaa taagtggttc
    11821 ataaccaacc ccttctaaca ttcctacagc ttcattaaat acttttaaat cttcttcttc
    11881 gacagctctg aattttgacc atccaccaac aaaaagactc atataaaaac ctcctttata
    11941 tatatattta tttatatata tagtagtacc aattttctca atattcaata ttttctgtgt
    12001 aactggtcaa tgaaaatgca taactggata aaaatactta taaaataact taactttaca
    12061 tataaatagc ttaatttaca tgtaataaga ttattctaca atatgaaata catcttattt
    12121 tgaatatatt ctaaatacaa taatggatat tttcaattat tatacttaga aaatatccat
    12181 tacttaattt gttttattta aaatcacata gagactttat tatttttttt attgcatgaa
    12241 tagctatttc aggtatcata aggtgatata ccccctcctt gaaaccacaa aaaacataac
    12301 aaactttacg aatcttgtct taaaagacca caggtacatt ccttatttgt ggattggagt
    12361 agccataaca agcaaatttt acaattttta tgatgacaaa aatcaataaa atcgcttagt
    12421 atataacacg aaatactact tgttgctatt aagaatagtt agataggaag tatgcattat
    12481 accaataaag gaatcaataa agaaagttaa aacatcccta caaattaaaa atctgttaca
    12541 tttttctata tttaaacgtt atttacttac aattggagaa ataacaggtt ttccttctcg
    12601 gtcaagtaaa ggcgtaaatc cggctgcatg gccataatag tggaatacat agttcacacc
    12661 tgtttcttta tccacccaaa tctccgttgc atttattttt ccttgtgaat atactttttc
    12721 aaatctatct tttcccattt tataatcctc catatttttg attggtctat gccttcttca
    12781 cttaagtaga aaaaatacat aacttatttt tttaattcta atatgagcac gtaaaaatgt
    12841 caaacagctt atttaatttc ataacttcat gttactgttc gtaaccttgt ctgtttactt
    12901 acactgcatt acagcaccat tttatccttt acattattgt attgcacaca gtaaaaatgc
    12961 ttactttatt ggatttttca actaaaaaaa ggattttttc tccacagaac atttactatt
    13021 ttttataata tatctaattt tactctcata tcaacattta atgaagatat aacctttttc
    13081 aaatattgta aagactcctt aaaaatatta gaaaaagatt ctgcaaatct ctcaacatct
    13141 ccttttttta aatacctaag tatctcaaaa catacatcaa aacgtgtttt caaaatttca
    13201 ttatattctt catgaaaaaa tctctctaaa tagattccaa atatcatata ttgttttaaa
    13261 tgtaaaaatg tttcctgtaa tacataaatt tgtgtataat ctactatctt acaaagtaaa
    13321 acaaaatctg gataaacctt acactcttca caattatttt gtaagtacct taaatgagtt
    13381 tctattttat ctacagctat caaatccatt ttatttccta gtaaaagtac tacatcctca
    13441 agagtaatcg taacaaactc catagcatcc aaaagcaaac tcaacctttt agctccttct
    13501 tctgtattta tataatcatc atcatattca gaaaaaagtg ttatatatgt tcccttgcca
    13561 ttaattgttt tagcaattcc caattcattt agctcattat aggcacttct aactgttgga
    13621 agagatacat tatatctatt aattatcgaa gtaattgatg gtagacaatc tcctatttta
    13681 atattgcctt caaaaatttg acaaattaca tctgaaacaa ttaagtcata taaataacat
    13741 ttttccaacg ttacagaatt aaaactactt gtatctatac tttgtggagc acgattttct
    13801 accattttaa gtatggattc tctacaagct ttatacatat atgttaatag ttttttaaat
    13861 tcaattagat tcccgtccaa tccatataag taagtttttc ttacctgctc tatgtattta
    13921 ttacgaaaaa agtctataca acaattatcc aagttatcca agttatcgaa attataggcc
    13981 aattgtgaaa taatgacatc atgctgaata atctcacaaa aatggtgaat caggaaatta
    14041 tttgttttac ttattagttt atttatatat tttagccttt gttctttaaa gactaaataa
    14101 ttagaactat ttttttccat acaatcgata atctcgttta attcttcaaa gtctttagat
    14161 gtaaatcttt taatattgta catagcaata gaaggaaata ttaaactaaa aatcttatat
    14221 atttctgata tttgtaaaat cttctcatat aataagtcag attctattga ttgtttactc
    14281 gtactctgga ttaaaactgg tctagaacca gcttttgtct ctataaagcc cttttctgag
    14341 agcattttta ttacagtttt tatagtattt tttgatacca tatattcttc tgacatgctt
    14401 tttatagaat caagcctatc tccaatagta attttaccag tataaatttg ttgaattata
    14461 tgctcaaaaa taattaaata ttgtggtttt ctcttcatat ttttcctctt cgataaatta
    14521 gatttattgt tttttgtaag gtatatttac ttttatttaa tagggcaaaa tagttgctta
    14581 ttttttcctc ataatctata attaatatag accatttata tatatggtct aagttttata
    14641 atactgtaat gcagtaaaat agcatattgt agctatacta cagcaaatta acataccatt
    14701 gtagtatatt aattggaata attttaagta cttcaaaatt ccagcaaaac atatatgtgt
    14761 ttttgatatt atgtatattc gttaatagaa tagctatact tgttttacat aataaattat
    14821 atgatacttg ctatatgtaa ttactatatt taatacacat aactattata cttactatat
    14881 gtaattacta tatttgatac acataattat tatacttact atatataatt actatacttg
    14941 gacacataac tattatactt gctatatgta attactatac tttatacaca tagttattat
    15001 acatgctata tataattact acacttggac acataactac tatatttact atatgtaatt
    15061 atactatacg tggacatata gttatcatac atactatatg taattactat acttggacac
    15121 ataactatta tatttgctat gtgtaattat actatacttg gacatatagt tatcatacat
    15181 actatataat tactatactt ggacacatta tcatacatac tatatgtaat tactatactt
    15241 atacacatag ttatcatata tactatatat aattactata cttggacaca tagtcatcat
    15301 acatgctata tgtaattact atacttatac acataactat tatactttcc atacataatt
    15361 actatatttg gacacatagt tatcatacat actgtatatt aagatataat actttttaaa
    15421 ttttatataa cacagacaca tgcaataaaa aagaaggtat ccataataag ctctttgcat
    15481 gtacctaaat aaaaactgta aaatagaata tataaaatct aatctatttt aagtaaatac
    15541 aatttcactt taaaagagga tgtactatga aataccatat atttttaatc ataactacta
    15601 tattaataat cgaactatgt aacatttaca tatgcaaaat agcatctttt agaaacaaaa
    15661 atataactat acataagaat tacaaatata tttttttaat aaaaccatta accttcttga
    15721 taataatatt cacaaaatta ccattaccac tatattatat attgttttac atagtaagtc
    15781 ataaattgct cttaagcaga aaaagttcta ctaacaacca ttttattccc aatatggcag
    15841 taataagttt ttgtatttta caccttatta ctgtaagtac aatatcactt gttaaaaata
    15901 caagtccaga tataatattt gcaaatatga cctataagtt aattagtatt ttaatagctt
    15961 ctattttaga ttttatctta tggtactcta tgaataaaat aaaagagcat atttatataa
    16021 taactcaaaa tcaaatagag ttcagtctat ttttaaaatt tatctggttc actttaggat
    16081 atatattatt tgatagtata caaattacat ttgctccacc aagttctcta acttcctact
    16141 ttatattggg aagtaacata ttattaatct tacaactaat tatattcatc aatcatatat
    16201 attccattga aaaaaatatt tatttggaaa aagaacataa tagactaaaa atgataaaac
    16261 aagaacaatt actaaaagta aatttattaa agaaaaaagc caacattgat gaattaacat
    16321 cagcatataa tcgaaaattt gcttttgaaa aaatacagca ttacattgat aacaatttat
    16381 tattttcatt gatatatatc gatttggata aattaaagac gataaatgat acatatggac
    16441 atttagctgg ggataactat attattaact tctcaagtat agttattgaa aacttaggaa
    16501 tagatgacat atttgctaga ataggaggag atgagtttat agttattatg cctaaatcta
    16561 atgaaaacac cacaaaagaa tttatagaga aaataagtaa aattatggat aatacgatat
    16621 cttttagcta tggtattgta gaagtttcta gcgaaaacaa aatgactgta gaagaaatta
    16681 ttaatattgc tgatattaga atgtacacta ataaacaaaa taatcataaa aggaacaata
    16741 actaaaatga atatttacat gagcttactt gtagtctcac tatgtttttt atcttattat
    16801 ctctatcatt ataatttttt taaatatctg gacataatga ttaataactc taaaaataaa
    16861 tacacagtat atattatata ttctatattt aattatttaa tttttattat ttgtacttat
    16921 ttaaagtttc atctaattat aaattggaca atttttatgt tgattttatt catacaaatt
    16981 aaagtaactt ataaaatctc cacaataaaa aacatttttt attcactaaa ctgctccatc
    17041 attggtttag caataaatat attttttagg tctttaatgt caatagtgtt aaatatgcca
    17101 gcatatttat ttgatagtaa ttctattgca aatatttaca gagtatttcc tatatcattt
    17161 agctttatag ttacaggact atatttcagt tataaaaata ataaaaataa taagattaac
    17221 tttgaggcag ttttagagga caaaaaaaac ttaattttct tattgagcct tatgtttgga
    17281 atgtacttgt atctatgttt gagtttgtca atatactata ctgttggaaa tgtattgatt
    17341 ttaaaattat atggaatcaa gtctgccata atagttttgg taggtaacta tattgcagaa
    17401 agatatagct ttagaattac tcaaataaaa ggttatgata ttaaaaatca aatagaatat
    17461 gtagaaagtg taaagacaga gtatgagatt aataaattga atgatattgt atatacagat
    17521 ttactgacag gtttctacaa tagacctttt gtgaatcaaa aactatcaga atggacatca
    17581 aatcactaca aatttacctt atgttttact gatttaaata gcttaaaacc tgtaaatgat
    17641 aaatttggac accaatttgg tgataaatac atatccacta ctgctcaaat aataaaaaaa
    17701 cttataaatg tagaaaattc cttactattt agatatggtg gagatgaatt tttattcctt
    17761 ttacagaata caagttgtgt agaagcagaa aagataatga ttaatataaa ttatgaatta
    17821 tcaaaaataa gtaataccga cgagtatcct tatgctcttt ccataagcta tggattagtt
    17881 gagtgggatg gaatatccga tattgaaact ttaatatcaa aggcagattc tttgatgtat
    17941 gaaaataagc ttaaaaataa atctacttta aactagctag ttcagatatt ctacaaaata
    18001 tatattataa gatagtacta agaatgtcaa aatatataga ttttgcaaat attgaataca
    18061 gtttactaat gaatgcactg gatgtaagtg ttagtaaaca cctattagat gaacacttta
    18121 ctgttgtttg ggctaatgac cgttattatg aaatgttcgg ctatacctag ctaaaaagtt
    18181 aaaaatgacc actgtagccg agggaataga gactagtttt caaaaagatt tcttgcaaga
    18241 aatagactgt gatatgttac aaggatacgt gttctctaga cctttaccaa taaaagactt
    18301 tgaaaaactt atgtttcaaa actcctccaa ttaacaatat aaaattgctt attataagtg
    18361 atattattgt atcaacaaat aaaggaattg tctcaaaatt gattttttct aatcaaattt
    18421 ttattttgag acattctcat ttttttgcat aagaaaatac ccaccaagct acggtttttc
    18481 ttaccgctgt tcggtgggta ttagtagtat tatgaaatct gaaaacttta agtattacta
    18541 cttccgctag cgcaatcaca aaggtcgaaa gcttcaaagc gaaaataggc tttttactca
    18601 tcaaacgtca ccgtacctgc caacccgtcc accgttatca tttgtccgct cttaatagct
    18661 cgtgtggcaa tgtgtgtagc cataacggct gggatgccat attcacgtgc gacaatgctt
    18721 gaatggctaa gaggaccacc aatgtccgtt acaatcgcac ttgcagatga aaataatggt
    18781 gtccatgctg gagttgtggt aactgcaacc aaaacacttc caggttgaaa gctttcaaaa
    18841 tctgctggac tattcagtac acaagctgga gccgtcacaa caccagtact ggtaccaatg
    18901 cccgtcaaca caattttccc atctttctgc ttctgttgtg cttgtgatat agtttttttg
    18961 tttttctctg gcaacttgga tggtgacaca taacccatgt attttttaag ttcagctttt
    19021 cgaacaggta ttatcccgtt catatcagat agagacttgt ttttatccaa ctgctctata
    19081 agtacctcca attccgattt tgtgagccaa taaatatcat ctaaatgtga aatagctcct
    19141 cctcgtatga atcgttctga gatttcctga aacatacaac gaatcagagg atgtcccatt
    19201 cccatcaaat aaatagcatt ttcacgcata ggagaggttt cctgtgccca atggagcaac
    19261 ttaaaaaata gttttttacg caatccacct atgtgttgta aaatctcttc ttctgcatgt
    19321 tttctacgct tttcaaacgt ggtctggcgt aaaaaaggac tttccccttt tccttccaca
    19381 aatgttttta cggattcaaa agttggtgta agtgtttcct gcggtgtgga atgcgcaaaa
    19441 tcaaactcat aagcggtacg accaaactct ttaaagtact tattgattcg atttttccat
    19501 tctatccata cttcctgtga aactccaaca ggcacaactg aagacatgaa atcttctgct
    19561 atctttattg caggactgtt ttgcagataa aggttgatgc tgttattttg ctttacccat
    19621 tctgaaatac cccacaactc cttttccgat tgcagaacaa tcgtatcaaa cccaagcagg
    19681 aaaacggaag tatccgttat accagcacgt cgaactgctc cttgaaataa ttttgtaaat
    19741 aatgtttcac tgatagaagc agcaggcaat gtaagctgaa tccttgtgaa ataaatacat
    19801 gcggcataaa atattgtctg aataccctcc ataatttgat gggcgttcat agtatgcagg
    19861 ggcttttctt cccattgttt aatagcatcc tcatatcctt ttcgtgccgt ttcccagcgt
    19921 gcaacgctgt tcataagtgt cctacgtaaa gagcgtgggg aaagggattt gatagcaatc
    19981 aaaagtggat ttgatttagc agaaagataa acatatccat taataaccgt atatgccccg
    20041 ttttttggta ataatttttt cgcactttta ccaaacatat ctatccataa aagctctgat
    20101 gcacggttta cgatttcaag accaagcgtg gcgaacaaag gtgtaacagg atttggcaaa
    20161 tgttctgcca aacttccctt tgtgtataca acatcctgtt ctggcaatac ccattctggt
    20221 ggcagaacag taattggacg agcctgaaca atgtacaatt tatctttttc cagtgcccac
    20281 tctacatcca taggcatttg ataatacttc tcaattttct ttccaagctg tatcagttgc
    20341 attacctgat tacgagtaag agcatatttt tttctcaacc tttcaggaac catggtttct
    20401 tctgttcctt ctgaagtacg aaccgtcata atctctttat ttgccacttc atacgatata
    20461 attcgttcgg agtccttatc cactacgatt gtatcgggtg taactaagct agaaaccacc
    20521 gcttcaccaa gcccccatgc agcgttaata atcatttcgc tacgtctacc attgattggg
    20581 tttaacgtaa acataatacc agatgaatcg gaaaatgcaa gcttttgtac tacaacagca
    20641 agtgcgacaa tctcgtgctt tatattattc ttcacacgat aagcaatagc acgagctgtc
    20701 cataaggaag cccagcatct ctttacagag tcaataactt tatcttcacc ttgtatgtta
    20761 agataagttt cctgctgacc tgcaaaagaa gcgtcaggta aatcttcagc agtcgcagac
    20821 gaacggacag ccaccgatat gttccctaat ccagcataag ccattttaat tgcgtccgac
    20881 acttcctgtg gcatttctcc attatggaaa agctccccta ttttcttaga tacatcttca
    20941 agttggctgg tattattgga atcaatacca tctagcagtt tattaattcg agactgaata
    21001 tggttcgtct caacaaaaat ctggtatgaa gcagtcgtaa catgaaaacc gtctggcaca
    21061 gggatgcctg ctgtcaatag ttttgataaa gacatccctt ttccaccaac aatttcaatg
    21121 gttgcttgtt tatgttccag tggcaagaca taataaatca taatcattca cctccatcat
    21181 tcataattcc gtgcagaaat atattcattg tttcatccaa cattttttcc gaactatcca
    21241 atggtcgtga cgtgtagctg attgataaca tggagttaat taacaaacgt gtcacaagag
    21301 cggtatccgt tttgcgaaag atgcctgtgg tcacaccatt atcaattacc gattttataa
    21361 tatcttgata tgcataacga gcatctttaa gtctctttcg atactcttct tctaaataat
    21421 cagattctgt atttaaccac atcagtacag ttctatactg ctctggaaat ctaagatgat
    21481 taagcatgat ttttctaagg cactgttgtg gtgaaggttc accatcaata atcctatgaa
    21541 cttgttggat agttttttct atttctttct caactgcata tacaacaatc tcgtctttcg
    21601 ttttgaaaaa atcgtataga cttgactttc ccatgttggc taaaactgca atttctctca
    21661 ttgatgtttt ttggaatcca ttttcagcca taagactcaa agcaacagca actatttttt
    21721 ctcggcgttc tgcttgttgt tcgggtgtaa gcacaattcc ttttggcata gggcgatacc
    21781 tccttttagc gaccactggt cgtttctttt ttatttatca tacagcgacc aacggtcact
    21841 tgtcaaggtt aatttttttc attgtatatg gtactcgaga taaaaatgtt cattttagaa
    21901 attgctctat ttaagtagaa ataggcttca tcattagaaa acaagactat aaatttgaga
    21961 tatttatcat gttcaaattt catcaatatc aaagtttaag atattaataa aaaaagaatt
    22021 actaagaaat aacatataaa gattcaaaaa aggagttgaa cttaaaattc aattacaaaa
    22081 aatcaaattt aagacaactc ctttattctt tatctctact attctttatc tctatttatt
    22141 tattacaact ccctaacatt taccactact gccttaggtc tagtcatagc ctcgattgaa
    22201 tacttaacac cttgagttcc cataccagat gcctttacac ctaaaaatgg gaagtggtct
    22261 ggacctcttt cagtcttatt atttatttga acagtaccaa cttcaagttt atcagctata
    22321 taaaatgcct tatcaatatc acttgtaaat actgatgatt gaagcccata ctctgattga
    22381 ttagcaattt caattgcttg atttatatct ttaactctta taattggaag tactggacca
    22441 aatggttctt cccaagcaat tttcatatca acatttacat tatctaacaa tgttggatag
    22501 aataaattat tttttctaac attgcctgtt attaactttg ctcctttatg taatgcatca
    22561 tcaaccaacc cttgaacaaa atcagttgct ttattatcta taagaggagt gataactact
    22621 tcatccattg gatttccaat tgtcaacttc tctaccaatg gctttatttt tccgactaat
    22681 ttatccgcca cactctcttg tactaaaatt ctctttactg ccgtacatct ttgtcctgaa
    22741 tatgaatacg ctccagatac tatattctta gctgcaaaat ctaagtctgc atcttctaat
    22801 actatagccg catcttttcc accaagctcc aataataatg gtaccattcc agatatttta
    22861 gatatatgct gaccaacttc agtactacca gtaaagttaa taaaattaat tcctttatgc
    22921 gttacaacat aatctcctat ctcactaccc ctacctgtaa tagtattaag tactccatct
    22981 ggtatcccag cttttctcat aatttctacc acatgaagtg cgctaatagc cccttgtgta
    23041 gcaggtttta atacaactgc atttccacca ataagtgctg gagcaatctt tgacattgat
    23101 aaatttacag gataattaaa aggagatata gctaaaacag tacctaatgg aacccttgaa
    23161 acataagaca tcttatttct tgttccacca gggaaattat ctccactaat ggcttcaccc
    23221 tccatatttt ttcccacatc agctgtgtat cttaaaaaat ctgctgttct ctcaacttct
    23281 gcccttgcag attttatgtc tttggctatt tcaagtacca gtatattagc tatctcatct
    23341 atattttcta acaataaatc agcagcctta tgaaatatat tggctctctt atatacaggc
    23401 atttcagccc atacttttat actctctttt gtgtttttta taacctcatc tacctcatgt
    23461 tttgtcatag attgtacttt cccaacaaga gaattatcta ttggtgagta aatttctatt
    23521 ggctttctcg aattagattc tacccatttt ccatttatta aatttttata tacattttct
    23581 tttgttttaa gttcattaaa catattttta ttcctctctc tgatataact ttgtgtattt
    23641 aaattatagc catttattcg ttatgtaaac atttcttttt ttcataaacg tttacctata
    23701 aaaaaatttt aagttaacgt ttttctacat tcttctattt taataacata ctcgctccaa
    23761 atctatatag gtcacacttt aatatcaata tactaatata ccaatatacc aatataccaa
    23821 tataccaata taccaatata aatattataa acataaatca cttgacaaat ttataagtag
    23881 acaatattat atatctatga cttaaaatat ttttaaagtc atagatatca tatatagata
    23941 gaaaggattg ataaaaatgg ctaaaagttt tacagaaata gaaaaagaca atataagaaa
    24001 aagactaatt tcagaatgtg aaatcaactg gtctaaatat ggatacaaaa aaactaatat
    24061 tgatgaactt tgttcaaaat caggaattgc aaagggttca ttctacacat tctttgattc
    24121 aaaagaaaag ttattttttg aagtattaaa tggaatccaa aacagactta tatcatcagt
    24181 agatgaaatt ttaagtaaca tgcctccaaa agaaggcttt gcaaaagcct taaagatgtt
    24241 gtatcgatta tatgataaaa acccattctt atattcacca aataatactg attacatcac
    24301 tttattgaat aagataccta aagagatgct ttcacaactt gaatacagca atgtacaaag
    24361 tttttatagc ttaattgaaa gacataatct caagttaaaa atagagaaag aaaaagcctt
    24421 tggtgtttgc aatgccctac tgtcaagcct attgctaaaa gaagatattg gatatgacta
    24481 cattgaagta tttgatttta tggtagacaa tcttgtagaa cacatacttg agtaataatt
    24541 taaaataaat tttctaaaca tttctttata actaggagga gtaatatgat tgaattaata
    24601 caaagactaa atgatattat ttacaaaata aatgataaac taaatatcca cacaaaactt
    24661 gtagacccta gcagtgagct catgccactt tactctatgt tatctataat cgtttctata
    24721 tttctaataa tattaggtat taaagtaaga aaaaaattcc ttataataat tgctattata
    24781 tcaattatta taaatatttt aataatctta gataggtagg tacatatatg gtaaaatcct
    24841 cgaacacttt taataaaaac ttcaacttga tgatattagg tcaaataatc tcattgtttg
    24901 gtgcatcaat acttaaattt gctttatctt tatacatact tgatataact ggtaaagcag
    24961 aaatatttgc tactatacta gcagtatctt caatcccaat tatagtattc tcccctattg
    25021 gtggagctat tgcagatata ttcaacagaa gaaacttaat ggttattttt gatttttcaa
    25081 gtagtcttac agtactaatc ttagcagtat tcttattcaa caataatggt tcaatattac
    25141 ttgtaggaat tattatgacc atattatcag tcataagcac aatgtatcaa ccaactgttc
    25201 agtcaagtac acccctttta gttgataatg agcatttaat gaatgcaaat ggtattgtag
    25261 ctggagttgc atctcttaca aatattgcag gacctgtatt aggaggtgtc ttgtatggtg
    25321 tgattggaat taatgcaatt atagttataa gttgtgtaag tttctttcta tctgcaatta
    25381 tggagatatt tatacagatt ccttttatca aacaagagca aaacagcaac attataaaaa
    25441 ctatatttac tgatataaag gatggtattg ttcatatatg taaagaaaat agatttatac
    25501 taaaaatact ttttttagct atagcaataa acttattttt aagctctatg ttaatagttg
    25561 gtatccccta tatagtaaaa atcactatgg gagctagtaa cttcttatat ggtctagcag
    25621 aaggagcaat ttcttttagt tcactggtag ctgctgttag tattggtatt ttctcaaaac
    25681 atctaaaaat cactaatttg catatttcct ttattatatg tgcagttatg ttcataccaa
    25741 tggcggtttc agtatatcca tttatattaa acacaggatt tccagttcca tttttaatct
    25801 ttatagtatc atctatgcta atatttttta caacgactct tatctcaata tatgttataa
    25861 cagtcacaca attaaaaact ccaaatgaac tcttaggaaa agtaatgtca attctttttg
    25921 caggaacagc aattgcaatg ccccttggtc aggttatgta tggtaaactt tttgatttat
    25981 tatcagatag agtgtacatc attatactag gagttggatt tattacatta cttatagcat
    26041 ttgtatcaaa gataacattg aggaacgaaa atctttagat acaaagaatt tagatataaa
    26101 gatttaattt tcactaattt ctgaatttaa atgcaaaatt gtgtatcagt atgttgacgt
    26161 tttagaaatt gattgatata atcaatggaa aataaacttt ataaaaattc aaaatattca
    26221 gctagaggag tgataaaaat gagttacatg ccaactttag aacaagcatg ggaaatatta
    26281 agaaaataca ataaggaaga atttcatata agacatgctc aaattgtatc tggagttatg
    26341 agatactatg caaaggagta cgaccctgaa agagtagaat tttgggaaat agttggactt
    26401 ttacatgatt tagattttga aatgtatcct gatgaacatt gtgttaaaca aagagaatta
    26461 atgacagagc ttgatttaga taaatcaata atcgattcta caataagtca tggatatgga
    26521 ttaactggtt cagatgttaa accagagcaa tttatggaga aagtattatt tgctgtggat
    26581 gaacttactg gtttaattgg tgctgctgca ataatgcgtc cttcaaagag cgtttctgat
    26641 ttagaattaa aatctgttaa gaagaaattt aaagataaat catttgctgc tggctgttct
    26701 cgagacgtta taagaagtgg tgcagaaatg cttggatggg aacttgataa gcttatccaa
    26761 acaactatag aagctatgag aagcttgatt ccagagatgg caatatagct cctccataca
    26821 tataccatag aagttacatc tttaaagagc atttctcttt ttgatgcaac ttctttttat
    26881 attttgtaga ataaaaagtt taatatttta aactcactat ataatctata ataaatacta
    26941 taaataatat aatgtataat agtca
//
LOCUS       DAMJVI010000046        25760 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.048, whole genome shotgun sequence.
ACCESSION   DAMJVI010000046 DAMJVI010000000
VERSION     DAMJVI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25760
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.048"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(269..985)
                     /gene="dapD"
                     /locus_tag="PC545_002008"
     CDS             complement(269..985)
                     /gene="dapD"
                     /locus_tag="PC545_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDF2456775.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     SNGSYIFVGDYEDIMEVLDKHGNDITDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(1209..1958)
                     /locus_tag="PC545_002009"
     CDS             complement(1209..1958)
                     /locus_tag="PC545_002009"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2456776.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDNELELVCVAARGLDSYEGDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDDEMNKIREAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            complement(2025..2912)
                     /locus_tag="PC545_002010"
     CDS             complement(2025..2912)
                     /locus_tag="PC545_002010"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2456777.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            complement(3029..4030)
                     /locus_tag="PC545_002011"
     CDS             complement(3029..4030)
                     /locus_tag="PC545_002011"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDF2456778.1"
                     /translation="MKKVNVAIIGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            complement(4455..5336)
                     /locus_tag="PC545_002012"
     CDS             complement(4455..5336)
                     /locus_tag="PC545_002012"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDF2456779.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENGTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPKDFAIYSGNDDTILPLLSLGGSGIISVLANICPKETHDL
                     VTKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKQELTNFGFKF"
     gene            5659..6852
                     /locus_tag="PC545_002013"
     CDS             5659..6852
                     /locus_tag="PC545_002013"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDF2456780.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            7058..8176
                     /locus_tag="PC545_002014"
     CDS             7058..8176
                     /locus_tag="PC545_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HDF2456781.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEVYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNIDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVSSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAIGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSKLLKYKEAID"
     gene            8307..8552
                     /locus_tag="PC545_002015"
     CDS             8307..8552
                     /locus_tag="PC545_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HDF2456782.1"
                     /translation="MKEKVQREKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            8539..9285
                     /locus_tag="PC545_002016"
     CDS             8539..9285
                     /locus_tag="PC545_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2456783.1"
                     /translation="MSQYSDFAFVYDELMNEVDYNGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKNYDIAGIDISDEMLSVAREKSEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKKDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENLIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLEK
                     SGFKDIKYYGDFTFDKPSEESQRIFFVCKK"
     gene            9570..10445
                     /gene="hslO"
                     /locus_tag="PC545_002017"
     CDS             9570..10445
                     /gene="hslO"
                     /locus_tag="PC545_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDF2456784.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYSEEELLEILKEM"
     gene            10836..11636
                     /locus_tag="PC545_002018"
     CDS             10836..11636
                     /locus_tag="PC545_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2456785.1"
                     /translation="MESKTKYIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRELGVKPQNVIILADIDKKEGTHYELMEKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREGKIKDDLYFVKPKYVKYIPRNERAIYKAMDFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            12163..14403
                     /locus_tag="PC545_002019"
     CDS             12163..14403
                     /locus_tag="PC545_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2456786.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNNIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFAESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYRSLI
                     NGYNIADLTSMQIDELAEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHSGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPSAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNVCEGKKFKKEVLEYRFQGKNIIEVLEMSVLEAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIS
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIELGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            14817..16352
                     /locus_tag="PC545_002020"
     CDS             14817..16352
                     /locus_tag="PC545_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2456787.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTIMTITGSILIALLALIFDFILGLVEKFFQRKRKKKNRKKV
                     FAILGSLILIATIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLNVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTESMFSKLQSEYKTKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNITDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVVVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            16368..17129
                     /locus_tag="PC545_002021"
     CDS             16368..17129
                     /locus_tag="PC545_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456788.1"
                     /translation="MTPIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKSAVNKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNTPANQFVDQLLNIRKSIIDDDNLKE"
     gene            17467..18336
                     /locus_tag="PC545_002022"
     CDS             17467..18336
                     /locus_tag="PC545_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2456789.1"
                     /translation="MDNNLITIEKTLDYIEKHLLDEMLDLNVISKEIGYSKYHLHRMF
                     TSVVGFSVHNYIQRRRLTEAARMLIFTEKPIIEIALISGYDTQRSFSRGFKKMFKRSP
                     NFFRRHQDFLPLQLKFDIHNRKKLRGDMVLDVKKVNCADINIVGYSGKVRKGFHIIGK
                     CWRDLHKNKEMIENKVDLGFLIGVSDYSNFKEIENTPTFTYIAGAQVATFGKLPNGME
                     KFVLPKSNYIVFTFIGKNEDSMQNVVEYIYQEWFPNSSCQFNENNLYDFVKYGEKTDE
                     NGLSEIQFWVPII"
     gene            18665..18916
                     /locus_tag="PC545_002023"
     CDS             18665..18916
                     /locus_tag="PC545_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDF2456790.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLETNDVVNWCKTKIE
                     SPNAIINRKGKNWYISVYDCIITVNAHSYTIITAHKEKK"
     gene            19028..19807
                     /locus_tag="PC545_002024"
     CDS             19028..19807
                     /locus_tag="PC545_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2456791.1"
                     /translation="MNDTVKEKLAESLTSDTTDILPYLPYLLQDFWELGSSPEQMLQL
                     FRKHIPFNEDTKVLDLACGKGAVSVKLAKELNIHIKGIDLIPEFIDYARRKAIEYKVD
                     ALCDFEMNDANSSVKIEHDYDCVIFGAVGDILGNYKETISKLLPTIKSKGFILIDDAY
                     VVDKSTNEQLNFERVYPTYNEWCILFKELGLKLIECIPCLELENDVEEEMNWIITRAN
                     ELIKKYPSQKDMFEQYVISQKNEYTDLQQDLVGATWILQKE"
     gene            complement(19942..20592)
                     /locus_tag="PC545_002025"
     CDS             complement(19942..20592)
                     /locus_tag="PC545_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456792.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSELKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIASPLYVDGIPSHLLHFLEKCEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWVQGIGIGAGEMFGSLESVPLGKGPKKDLGIALKE
                     LSKNILSDNIHIDDSNKSTIFISPNFPRFLYKLMGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(20589..21092)
                     /locus_tag="PC545_002026"
     CDS             complement(20589..21092)
                     /locus_tag="PC545_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2456793.1"
                     /translation="MRLLIHDLTDKELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPSECVVKDYYSNMGKLINDSDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKQINLKAIFYGDCITEAEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(21141..21656)
                     /locus_tag="PC545_002027"
     CDS             complement(21141..21656)
                     /locus_tag="PC545_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2456794.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGNKLKKLYSITNTGKEVFLEWLKKPINFSKTKQDHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKNDAEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            21936..22460
                     /locus_tag="PC545_002028"
     CDS             21936..22460
                     /locus_tag="PC545_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2456795.1"
                     /translation="MHEQIDCCERKKRTIDIKGENMRELLKWVSIINRYNTNYIDRKL
                     ADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGVK
                     DKRTWHLYPTEKSLGCYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDMN
                     EEAKLEAKENGRKI"
     gene            22444..23847
                     /locus_tag="PC545_002029"
     CDS             22444..23847
                     /locus_tag="PC545_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2456796.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIVVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQLFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(24411..24722)
                     /locus_tag="PC545_002030"
     CDS             complement(24411..24722)
                     /locus_tag="PC545_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456797.1"
                     /translation="MKKIIKSFTFWFFLIALFEIYMHQIGQDSKSIVLIGLNPILSII
                     SRVDSFFVFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(24778..25371)
                     /locus_tag="PC545_002031"
     CDS             complement(24778..25371)
                     /locus_tag="PC545_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2456798.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEINDNGLLPGSRQHIAGANYTVEIMKQAPVTIFIL
                     NILGKSPLEKLSPEERFYEMANMQSIGAAIQNMSLTAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLSDVTEWR"
BASE COUNT         9578 a         3156 c         4244 g         8782 t
ORIGIN      
        1 cactttatta aattataaaa agtttaatag tttattacat tgatatattg aaaaattgaa
       61 ggaaataaaa atacttgtca gcaagaaata aaaatgatat aagaatgaat aagagaattg
      121 taaaaaaata aaatgattat aaataaaaga taaaaatatt gtaaaaaaga gggtgcctct
      181 ggtgaattaa attcacacag agacaccttt ttaattaatt ttttaaatta gtatattatt
      241 gtttaattaa tagcatagta ctatttaatt aatctaaatt tctaaggtct tccataagct
      301 tagttttgtc agccgttttt tcatctttca tttttataac tttagcagga gaaccagcaa
      361 caacggcacc agcttctaca tcagtagtta caacagcacc agctgcaaca acagcacctt
      421 taccgattct aacaccttca agaataactg cattagctcc tattaaaaca tcatcttcaa
      481 ctataacagg agtagcagaa ggtggctcta aaacaccagc aacaacagca ccagcaccta
      541 agtgaacatt tttaccaaga gttcctctag ctccaataac agcattcata tctaccatag
      601 aaccttcacc aataacagct cctatattta taacagctcc catcattaca acagcatttt
      661 tttctatagt aaccatgtct cttattattg cacctggctc aattcttgca tgttcactta
      721 aagtattttt tagaggaata gcagaatttc ttctatcata ttctacatga gtatctgtta
      781 tatcatttcc atgtttatct aatacttcca ttatatcctc atagtcacct acaaatatat
      841 aagaaccatt acttccaaat accttaaatt tatcagttga ttggaagtgt aaagagttag
      901 tatttacata taccttaaca ggtgttttct ttttaacttc ttttatatac cttgctattt
      961 cataagcatc gtttaaattt accatttgtt aattctcctt gtatttcatt atatttcttt
     1021 tattatatac ttatttgtca attttagtat atacttactt cttattattt atattttatt
     1081 ttagttagct tttattgtat agacatagta ttaaataaat ttattatata tgtttgattt
     1141 gtattgcata ttattatata tatattgtat atgtttaatt tatattgtgt attattatat
     1201 ttatactatt atcccaacat gctgtccata ttataaaatc ctggtttttg cttaactaag
     1261 tatttggcag cagctataga accacttgca aagataccac gagattgagc ttgatggctt
     1321 attgtaagaa cctcattgtc tccagcaaat agagcatcat gttcgccaac aatatttcca
     1381 cctcttatag catgaatacc aacttcattt ggttgtcttt ttgcactacg accatatcta
     1441 ccataattat tttcaacgtt tggcaaaacc tcttttatag cgttagcaat cataacagct
     1501 gtgccacttg gggcatctac ttttctgttg tgatgttttt ctattatttc tatatcaaat
     1561 ccttctagta atttagctgc atctttaacc aattttacta atacatttac accaagagac
     1621 atattatatg attggaatac aggaataact ttagccgctt cacgtatttt gttcatttcg
     1681 tcatcattat atccagtagt agcaattact aaaggtgttt tagtctttaa aacgtaagat
     1741 aagatattat ctaaattaga gtgatgagaa aaatctataa ctacatcagc ttcctcaact
     1801 atagtactca tatcttcata aattttaagg tcgccttcat agctatccag acctctagct
     1861 gcaacacaaa ccaattctaa ttcattatct tctcttatag tatcggctaa aatcttaccc
     1921 atttttccat cataaccagt tgctataact tttaacataa tttcctccta tgattaaggg
     1981 ctaacttttg accaaactta ctttttacca tgcattaatc tttactatgc attaactttt
     2041 agtccaaagt taactaattc tttctttaat acttctaaat ttgctgggtc catttctgct
     2101 agaggtaatc ttaaatctcc aacattgaat cccaataaat tcattgcagt cttaactggt
     2161 ataggattta cttctataaa taaagctgcg attagagcat ccataccaag ttgtaatttt
     2221 ctagaacctt caatatcgcc ttcaaagaat ttagctacca aatcgtgagt ttcttgaggg
     2281 caaacattag caagtactga tataactcct tgacctccta aagataataa aggaagtata
     2341 gtatcatcat taccagagta gatagcaaag tcatctggaa ctaaactagc tatttctgca
     2401 acctgtccta ggtctccact agcttctttt acagcaacta tgttatcaat tttagcaagt
     2461 tcaactataa ggctaggact tatatttaca caagttcttc cagggacatt gtaaagtata
     2521 attggaagtt taacactgtt agctattgtt tcaaaatgac gcttaagtcc agctttatta
     2581 gctttattgt aataaggtgt tattattaat agagcatcta ctcctaattt ttcagcttct
     2641 tgacttaagt taactgagtg catagtatca ttagaaccac ttccagctat aacaggaact
     2701 cttttattaa ctttttcaac aacatactta atagtagcaa gttgttcttc atcagtcata
     2761 gtattagctt caccagtagt accacaagat actatagcat cagtaccatt agctatttgg
     2821 tattcaacta attcacctaa tttgtcaaaa tcaactttgt tatcctttgt aaatggtgta
     2881 actagagcaa cagcagaacc tttaaatatc atataaaatc aatcccctct tttttatttt
     2941 tgtgttttat attacagtta cttctaatgc ttgtttttga gttactatta atattttaca
     3001 aaattatata tttatacaag tttgtatatt atactaaatc gtatgataaa gccatttcag
     3061 ctatttgaat tgcgttagtt gcagcacctt ttcttatatt atcagcaaca acccaaagat
     3121 ttacaccatt atcaacacta aaatctcttc ttattcttcc aacaaatact tcatctctat
     3181 catcaaattc tacagcagtt ggatattcgt ttttagcagg attatcaact actacaacac
     3241 ctggagcttc ttcaagtgtt tttactaaat catctaactc aaatggattg ttaaactcaa
     3301 catttataga ttcactatga ccatttttta ctggaactct tacagtagtt gcagttattt
     3361 ttaaatcgta gttatcaagt attttcatag tttcatttac cattttcatt tcttctttag
     3421 tgtatccatt ttcagtgaat gaatctatat gaggaagaca gttgtaagag atttgatgag
     3481 ggtagaatcc agtaggatta ccgttcattc ctttctctaa atcttcaaca cctttaacac
     3541 cagaaccaga tacagcttgg taagtagagt aaacaactct cttaattttg tatttatcat
     3601 caagagcttt aaggggaacc atagcttgta tagtagagca gttaggatta gctattatac
     3661 ccttatgatt ttttatggct tctggattaa cctcaggaac tactaaaggc acatctggat
     3721 tcattctcca ttgactagag ttatctacta ctaagattcc atttgaagca gctattggag
     3781 catacttttc actaatactt ccaccagcag agaataatgc tatttgtatg cctctatcag
     3841 taaatgaagt ttcctttaat tcttctacta tatattcttt accacaaaac attactttag
     3901 aaccagctga tttagaagaa gagaataaaa atagattttc tattggaaaa tttctttctt
     3961 ctaatacttt taaaaatgtt ctacctacca ttccagtagc accaattatt gctacattta
     4021 cttttttcat ataaattcac cccaatttta aattattaaa acattatttt attttagtta
     4081 tacaagtaag atatgttaat atttttatat atccaaaaac tttaatatta tattaacaac
     4141 tttaattata tacaaaaaac gtacaaaggt atactttgta cgtctaattt atcactcaaa
     4201 gtaaaccctg tagctcatca cctacaaaaa taagtgacag tattacacat gttatgtgca
     4261 atcccagaaa atattattgc aagaaatatt ttcttcggct gtaaatcctt tttccaatca
     4321 ttaacacctg ccagctacta ttggatactc ttagttacag ctacctctac cctaggttaa
     4381 taggatatta aaatttgatt attctaataa taatacatat ttaagaaaaa atcaacttat
     4441 ttatataaat attgctaaaa tttaaatcca aaatttgtca attcttgttt taacacatta
     4501 agattagttt cttccatttc agctagagga agtcttaaat caccaacatt aaaacctaaa
     4561 atgttcatgg cagttttaac aggaactgga tttacttcta taaataaagc agcaattaaa
     4621 gcatccatat caagttgtaa tttttttgag ccttcaatat caccttcaaa gaatttagtt
     4681 actaaatcat gtgtttcttt aggacatatg ttagcaagta cagagataat accacttcca
     4741 cctaatgata atagtggtag tatagtatca tcattaccgg agtatatagc aaagtcttta
     4801 ggtactaatt tagctatttc tgcaacttga gctaagtctc cacttgcttc cttcacagca
     4861 actatattat ctattttagc aagttcagcc acaacagatg gctttatatt cactttagtt
     4921 cttcctggta cgttgtataa aataattggc aacttaacgc tatttgctat tgtttcaaag
     4981 tgaagtttaa gacctttctc attagtctta ttatagtaag gagttattat taaaagacca
     5041 tccaccccta atttttcagc ttcttgactc atatgaacag acaacatagt attgtttgaa
     5101 ccagtacctg ctataacagg tattctctta ttaacttttt caactgtata ttttattaca
     5161 gcaaaaattt cagattcact catagtagtc gcctcacctg ttgttccaca aacaactagt
     5221 gcatctgtgc cattctcaat gtgatattcg attaattcac ctaatttttc aaagttgaca
     5281 ttattatcct ctgtaaatgg ggtaactaaa gcaacagcag aacctttaaa taacatataa
     5341 tcaaatctcc ttttttatct aaatttaatt ttttaagtac aataataatt atagtacaaa
     5401 ttaattaatt ttaataggta tagttaatat cttaactgtt taaataagat atttatatta
     5461 tattaatatt agaaaaattt taaataaatg ttttattctc aaaatatgca tattaattta
     5521 ataaaatata ccaaaatgga aataaaaaat aaattttatg agaatttaat aactaataat
     5581 taatatattt aggttataat aaatatgtgc gtaaaatcta acaataagca ataataccgt
     5641 aaatttagga ggaataatat ggatacttta aaggaacttt ttaaaatagg aagtggacct
     5701 tcaagttctc atactatggg accacaaaga gcagcagaga gatttaagaa tgaaaaccca
     5761 gatgcagaaa gttttagagc aatattatat ggtagtttgg cagctacagg gaaaggtcac
     5821 ttaactgact atataattga aaaaactata gcacctaaaa aagtcgaaat tgtttgggaa
     5881 gaagatatta taaaagattt tcacccaaat gggatgaagt ttgaagcttt agataaagat
     5941 aaaaatgtaa cagctgaatg gactgtatat tctgtaggtg gaggaactat agcagaagaa
     6001 ggtcaaagaa acagtaaaag caatagtata tatcacttag atactatgga tgagatagta
     6061 aaatggtgca aagaaaataa taagactttg gtagattttg tgttggaatg tgaaccaaaa
     6121 gacataaaag attatataaa aactataaaa gatgctatga gaaaatctat tgatgatgga
     6181 ctaagtacag atgaaataat accaggcaag ttattattaa aaagaagagc aagtacattt
     6241 tataatgcat ataaaaaaga taagagcttc tcaacacttg tatatgcata tgcactagca
     6301 gcttcagagc aaaacgcttc tggtaatata atagttacag caccaacttg tggttctgct
     6361 ggagtaatac caggaatatt ctttgcaatg caggatttct ataattatga tgacgaaaaa
     6421 ataattgaag cactacttgt agctggaata ataggtaata taataaaaac taacgcttca
     6481 atatctggag cagaggtagg gtgtcaaggt gaagttggag ccgcttgttc tatggcagca
     6541 gcagcagtag catacttaaa aggtggaact atagaccata ttgaatatgc ggcagaaata
     6601 gcacttgaac atcatttagg aatgacttgt gaccctgtat atggatatgt tcaaattcca
     6661 tgtatagaaa gaaatgcaat ggcagctcaa agagcttatg atgcagctaa ttatgcatta
     6721 ttaactgatg gctcacattc tgtctcctta gaccaagttg ttgaaacaat gaaagaaact
     6781 ggtatagata tgatggataa gtacaaagaa acagctaagg gtggacttgc taaacatttc
     6841 ttctcttgct aaaaataaaa ttttccaata ataattcctc tgttttgatt tatgaaacag
     6901 gggaattatt attaatggtg aattatttaa taatgcaaaa tatttttata ttcaatttag
     6961 ctttttattt ttaattagta taacatgcta tataatattt taattataaa gaaatataga
     7021 aatatttttt aataaattag aaattggagg agattacatg agagaggcat catatttaca
     7081 agaacaactt tataaacata ggaagtcatt aaatcctatt gcagagattg ggagaaaaga
     7141 gtataagact tcaaaatata taagagatta tcttgataaa ttaggaattg agtatgaagt
     7201 ttatttagaa acaggtatag taggtgtaat agaaggtaaa agacctaaaa aagatattgc
     7261 ttttagagct gacatagatg gtcttcatag tgaagaaggg gtaaaacatt tatgtggtca
     7321 tgatggacat gctactatct tacttggatt aattgagtat ttaaatgata ataaagaaga
     7381 cttaaatgat aatatagtat ttatattcca accggcagaa gaaggtcctg ggggagcaaa
     7441 agctttaata gatgaaggag tgcttagaaa atacaatatt gatgaaatat atgggcttca
     7501 tatatatcca gagttaccag aaggttatgt aggtacaaga gagggctatt ttatggctca
     7561 aataggagat attgacatag aaatagtgtc taaaagtgga catggggcta tgccacaaaa
     7621 tggtattgat ggtatagtga tagcttcaaa ctttgtaagc tcacttcaaa ctatagtgtc
     7681 tagaaatata agcccaatag acaatgcagt gttgactatt ggcagaattg aagggggagc
     7741 tagaagaaat ataatagctg aaaatataaa gatggaaggt actatgagat gtttcagccc
     7801 agaagtgtat gagaaaatga agcttagagt tagagaactt gctagagggt ttgaattggc
     7861 ttataactgt aaggttaata taaatattat agatgattat atcgctgtta aaaatgacaa
     7921 ggatttgtat caagagtttg tagaggctat tggaagtgat actgttgtag agttagagcc
     7981 acttatgata tcagaagatt tttcttatta tcaaaaagaa gttccaggat tattttttat
     8041 gttgggttca agaaacgaag aaaaaggatt tgtaaatggt cttcataata taaactttaa
     8101 ttttgatgaa aagatatgtg taaatgcatt gaatgtttat tcaaaattat taaaatacaa
     8161 agaagcaata gattaggaag ataagttata aagattaaaa agcataaata cctaaattta
     8221 aaaaaagtgt attaataaat accccttaca tatttgtata aatttatttt aattgacaaa
     8281 aatacaaata agaaggggtg atttttttga aagaaaaagt acaaagagaa aagaagataa
     8341 ctaatggcaa gaatactaaa atattgacaa atgatgatat aatgaaatat gagatagcct
     8401 cagaactagg tcttatggat aaagttggtg aacgtggatg ggcaggactt acagctaagg
     8461 aagcaggaag aattggaggt atgcttacat cacgcaaaaa gaggcttaaa aagattgtgg
     8521 aggagcagaa taaaaaggat gagtcaatat agtgattttg cgtttgtata tgatgaactt
     8581 atgaatgaag ttgattataa tggttgggtt aaatatatag aagatataat agatagtgaa
     8641 ggtgtaaaag taaaaaatat attggaattg gcatgtggca cgggtaatct tacaattcca
     8701 ttgactaaaa aaaattacga tatagctggt atagatatat ctgatgagat gcttagtgta
     8761 gcaagagaaa agtcagaaaa agaaggagta gaattagttt tacttcaaca agatatatca
     8821 gagcttgatt ttgaaatatc tgatttggac tgtgtacttt gtgcttgtga tggattcaat
     8881 tatataactt atgatgatga tttggaaaat gtattttcaa agacatatga attattaaag
     8941 aaagatggaa tattcatatt tgatataagt tctttttata aattagccaa catattggga
     9001 aataacatgt atggagaaaa tagagaagat atagcgtata tgtggcaaaa ttactttgat
     9061 gaagaagaga atctaattga aatggaactt acattcttta ttaaagatga atgtggaaaa
     9121 tttgaaagat ttgaagagat acaccaacaa agagcatata ctgaagatga gatagttgat
     9181 atgcttgaaa aatctggatt taaggatata aaatattatg gggattttac atttgacaaa
     9241 cctagtgaag aaagtcaaag aatcttcttt gtatgtaaaa aataagatat ttttgactat
     9301 ataagataat tattaaaaat aaccatgata taaaaatata gaaaatgcag ttttatagat
     9361 taaaagtaaa gtgatgatac tttatgactt ttatttttta ataattataa agttctatag
     9421 ataaaaatta aaatataata actattgctt agtaaaatta aaataaaatg ggttttgccc
     9481 aaggttaaga tagataagta tttatagatt aaaattaagg tagtaatata ataaaaataa
     9541 gacaattaac gggacatgga ggaatattaa tgagagatta tgtactaaga gcgacatcag
     9601 gaaatggtca agttagagca tttgtcgcaa ctactagaaa tacagtagaa gaagctagaa
     9661 gattacatga aactacaaaa gtagcaacag cagcacttgg aagaacatta acagccacat
     9721 ctataatggg attaatgatg aaaaatgata gtgataaatt gactgtaata ataaaaggtg
     9781 gaggtccaat tggaactatt atcgccacat cagactctaa gggaatggta aaaggatatg
     9841 taggaaatcc acaagtagag gttgaagact atccaaatgg aaagttaaat gtagcagcag
     9901 cagtaggtac agaaggtgtt gtaaaagtta taaaagactt aggtcttaga gaaccatata
     9961 atgggactta tccattggta agtggagaaa ttgcagaaga ctttacttat tattttgctg
    10021 tatcagagca gactccatca gtagtagctt taggtgttct tacaaaagaa gatgaagtag
    10081 agtttgctgg aggatttata gttcaattaa tgccagatgc agaagaagag actattgcta
    10141 agttagagga aaatgtagct aaattacctt ctataactaa catgttaaaa gaaggaaaat
    10201 caccagagga tatattaaat attgttttgg atggtttgga acctaaaata cttgatacct
    10261 gtgaagttgg gtttatgtgt gaatgttcaa aagaaagagt aaaaactgct ttggttgcaa
    10321 ttggtaaaaa atcattggct caaataatag aagaagataa aaaagcagaa gttggttgcc
    10381 agttctgtaa taagaaatat atgtatagtg aagaagagtt gcttgaaatt ttaaaggaaa
    10441 tgtagactat aaaaatagtt gtattgatat aagtaatgtt atagtgatat agtaaagtcg
    10501 tattgatata gtaattaaag tataatttat taataataga catatttata ttaaaaaata
    10561 agtttaataa atttttaagc ttaataaatt ttaagaatta agaagctttc tggctgtaat
    10621 gtttgcaatg ttgcagttag aaggcttttt tattttatta tagttcgtgt caaagattgt
    10681 atgaaacaat aaaatattgt aaaataattt gtatatttag acctgattgg cacaaaggtt
    10741 tataaggtat ttgagttgat tatgcgaaat tctatgaagt agtaaccctg tgactttaat
    10801 catgggaggt tcagaagaag gagaaaattt taaatatgga aagtaaaacg aagtacatag
    10861 gaataagtat attaatatta ttgatgatat ttttaattgc aaaaaatttt aacggaaaac
    10921 ttgaaatacc tattcctgag agtaatagta gaaaatttaa atcagcagca gtgttttatc
    10981 ctcaacatca agatgatgag gttttatggg gaggaagtgc cattgtagat gctataaaac
    11041 agtgtggtgt tgataatgta tatgtggtat tagtatcaga tggttctgga gttaacgttt
    11101 tcaaagctaa cacaaaattt agaaatttaa ctaggaagca aaaggaagaa cttagaaata
    11161 acgagtttaa atcagctttg agagagcttg gagtgaaacc tcaaaatgtg attatacttg
    11221 cagatataga taaaaaggaa ggcacacatt atgaattaat ggaaaagact atattggatt
    11281 ttgaacataa gtttaaaggc aatgtgaccc atatagccca tcactataag tatgatgacc
    11341 atataatgca tagaaagaat ggggaagttt taaagaaact taaacgtgaa ggtaagataa
    11401 aagatgactt gtattttgtt aagccaaaat atgtaaagta tataccaaga aatgaaagag
    11461 ctatctataa agcaatggat tttgatgact acaataaggt taagagtgct tgttatgaat
    11521 ataaggttgt aaattcaaag cgtaaaatgt atggtatagg atatatttcg gcacatagtt
    11581 attttgaaca tcttctaaat gaccctgaac ttacgtctgt attaagtgaa gattaatttc
    11641 ttataagtag taagttatgt ttgataagtt gattggaatt ttctatagtt ttgaaggtat
    11701 tggaaattta gttttattta aagatttaaa gatagaggtt tttagtgttt aaaattttaa
    11761 agttaagttg aataattgta tagaattatt tgtgaaagaa gaaacagtgt aaaacatagt
    11821 atcttattta tggttttaaa atcataggca agatactttt tttatgtaac taataccatt
    11881 ttgagattat taacttaaga attttatata atttaacact catatataat gatgatattt
    11941 aggtagaata gatgcaatta cgcttaaata aaataactgt attttttata aaaatcaaac
    12001 cctacactat actaccagat aaaatctaca tgctataatt tgacctgtgt ttataaaaaa
    12061 aggagtaatc taataaaata gtatttcact tatgtataac taacaactat tattacgatt
    12121 ttctctaaat aatttaaaga aaaataatgc aggaggtaaa atatgaacaa tgaatttata
    12181 attttaaaag gttgcaaaga gaacaatttg aacaatattt ccttaaaaat accaaaaaga
    12241 aaaataacca tattcacagg ggtgtcgggt tctggaaagt catctattgt atttgagaca
    12301 attgcaaaag aatcacaacg ccaactaaat gaaaggttta gtacatttgt aagaagtttt
    12361 ttacctaagt atggagaggt taaagctgat tgtatagaaa atttgtcaac accaattatc
    12421 atagaccaaa gtagacttgg tggaaattca aggtcaaccc taggtactat tacagatgta
    12481 aattcctttt taagagcttt atattcaagg tttggtagtg agtatatagg taatgctaat
    12541 atgttttctt ttaataatat tgatggtatg tgcccacact gtcatgggct tggaaaaaag
    12601 ctagttccta atatgaacca aattcttgat atgaataaat cattaaatga aggtgctata
    12661 ttgctttcag ggtttggagt gggttcttgg cattggaata tattcgctga atcaggattt
    12721 tttgatatta ataagaggat atgtgattat tcagaagtag aattggaaaa gtttttgcat
    12781 ggtaaagcag aaaagattaa gatagaaaat gctggtcaaa ctaacatgac atatgaggga
    12841 ttaatggtta aatttaatcg cctatactta agtagagagg gagaaatatc tgaagctact
    12901 aagaaaaaat tgagtaaact tcttatagaa gatagatgtc ctatatgtga tggaagaaga
    12961 cttaatgaaa gagtatatag gtctttaatt aatggttata atatagcaga tttaactagt
    13021 atgcaaattg atgaattggc tgaagttatt aaaagtatag atgagccaga agcacaacct
    13081 ttaattaaag gaataataga aaagttaaac agtatcatag atataggtct tggatattta
    13141 acactggata gagagacatc ttctctatca ggaggggaat ctcagcgtat aaagatggtt
    13201 aagtatttaa atagtaatct tgttgattta atgtatattt ttgatgaacc aagtgtagga
    13261 cttcatccaa aagatgtgta taaattaaat aatcttttaa aaaaattgcg tgataaaggg
    13321 aatacaatta tagttgttga acatgaccca gatgttatta agattgcaga ccatattgta
    13381 gatgtaggac caaaggcagg taagtatggt ggagaagttg tgtatgaagg cagttatgaa
    13441 aatctactta caagtggtac tttgacaggg aatgctttaa gtaaatttct tcctattaaa
    13501 gaaagtgtaa gagaacatag tggatattta gaagttaaaa attgtaataa aaacaattta
    13561 aaaaatgtat cgattaagat acctaaaggt gtattgactc tagttactgg agtagcagga
    13621 gcaggtaaaa gtacgttgat taaggatgaa tttttaaagc aaaatcctag tgctgtactt
    13681 atagaccaaa gccctgtttc agcaaattca aggtcgagtt tggcaacata tagtggaatt
    13741 atgaacaaca taagaagcat attttcaaaa acaaatggcg taaatgcttc tttattcagt
    13801 tcaaattcag aaggagcttg tgataactgt aagggttctg gaatagtaga aatgaatctt
    13861 gcttttatgg agagtataaa gagtacctgc aatgtttgtg aaggtaaaaa atttaaaaaa
    13921 gaagttttag aatataggtt tcaagggaag aatataattg aagttcttga aatgtctgtt
    13981 ttagaggcaa ttgagttttt taatttaaaa cagattaaaa cgaaattaca atgtattgaa
    14041 aaaatgggta ttggatacct tactttagga caaactcttg atactttgtc tggaggagag
    14101 tgtcaaagat taaagcttgc tagtgaatta aataaggaaa gttcagtgta tatattagat
    14161 gaaccaacaa caggtcttca tatggctgat attgagaatt ttataagtat aattgaagag
    14221 attgtagata atggaaatac ggtcataata attgaacaca atatagatgt aattaaaaga
    14281 gctgattgga ttattgagct gggacctgaa ggaggaacaa aaggcgggag agtaatattt
    14341 gaaggtattc caaaacaatt atgtaacaat aaaatatcat taacagcaaa atatatagta
    14401 taataaataa aatataataa aattataatg atattttaat aaatataatg ggtatataaa
    14461 atgataatta aacttaacag taataaataa tgtgagaaat attcaaattt aattaaataa
    14521 agtgtcagaa atttgtttaa attttagtat tttaacggtt ctgacctttt ttcttatttt
    14581 tatggagaat atcgaggatg gagtctctaa tagttacttt tttgtgtcta cttgcaagtt
    14641 tgattaaaaa atggctatac acacttgtga cttcagtcat gggaggttca gattataatc
    14701 ttaatatata atttgtgaaa taatttgatg acgatttgct gatgtataaa caataaagat
    14761 taactacatt aaaatttaaa tcttgttaag taaaacttat aaaaggagag agatggatga
    14821 taaatgatat tctaacctta ttatctgaaa gaagtgattt ttttataaag ctatccatag
    14881 aacatttgat gatttctatt atttctataa taattgcgat tgttttgggt ggtataattg
    14941 gtattattgt aagtgaattt cataattctt ctaaacctat attgggaact atcaattttc
    15001 tatacaccat tccatctatt tctatgctag gatttctgat accattttcc ggaattggaa
    15061 atactactgc tgttattgca cttaccattt atgcattatt gccaatggtt agaaatacat
    15121 atactggaat tactaatgtg gataaaaata ttcttgaagc agctagggga atgggaagta
    15181 cagattttca gatattgtat aaaataaaac ttccgttagc cctacctgtt attatgtctg
    15241 gtattagaaa tatggtgatt atgacaattg cattagctgg tatcgcttca tttataggtg
    15301 ctggaggtct aggtgtagcc atatatagag gaatcacgac aaataacaca ataatgacaa
    15361 ttactggaag tatacttatt gctctattag ctttaatatt tgattttatt ttagggctgg
    15421 tagaaaaatt ttttcaacga aaaagaaaaa agaaaaatag gaaaaaagtt tttgccattc
    15481 taggaagcct tattcttata gcaactatct ttacgtctta tttatttaat ccttcaaaga
    15541 ctcaaactat tcatattgct actaaaccta tgactgaaca atatattctt ggtgagatgt
    15601 tggatatttt aattgaaaaa aacacaggtc taaacgtgga aattacacaa ggtgtaggag
    15661 ggggaacatc taatatccag ccagctatgg agaatggaga atttgacatt tatccagagt
    15721 atacaggtac aggctggaat atggtactta agaaaaatga attatatact gaaagtatgt
    15781 ttagtaaact acagtcggaa tacaaaacta aacttaaaat gcaatggact ggtatgtatg
    15841 gtttttctaa tagctatgca ttagcagtta gaaaagaagt tgcagataaa tacaatttaa
    15901 aaaatataac ggacttaaaa gatgtatctt ctaaattgac atttggagct gaatatgact
    15961 tttatgagag agaggatgga tatactccat tgtgtaatac atatggactg aactttaaag
    16021 atacagttga tttagatatt ggattaaaat accaagctat caatgaaggt aagattgacg
    16081 taatgcctat atttacaact gatggtcagt atagtgtttc agatgttgta gttctaaaag
    16141 atgataaaaa tttctaccca tcttatcaat gtggaaatgt tgttcgtttg gaagtattag
    16201 aggaaaatcc agaactatca aaggtattta agatggttgg agatattttg actgataaag
    16261 aaatggctaa aatgaattat gacgtagaaa acaacaattt agaacctaga gaagttgctc
    16321 gtgagtttct gaataagaaa ggtcttttat aaattggagg aaatataatg acacctatta
    16381 tacaatttaa aaatattaaa aaacaatata atgataaaac tataatagat aatcttaatt
    16441 tagatataga aaaaggagag tttttgactg taattggttc gtctggttct ggtaagacaa
    16501 cactcttaaa aatgattaat ggtctgattt tgccagatgg aggaaatatt ttgataaacc
    16561 aaacagatat caaaaacgag gacttaatta aattaagaag aagaatagga tattgtgtac
    16621 agggaagtgt tctttttcct catatgactg ttgaagaaaa tatttcatat gtaccaaatc
    16681 tcctaagtaa aaagaataaa ttagaagtaa aaagtgctgt aaacaagtgg atggaaatag
    16741 tgggattgcc aaatgatatg aaagtgcgtt acccatcaga attgtcaggt ggtcagcaac
    16801 aacgtgtagg gattgcaaga gctttagctt cttctccaga aattttatta atggatgaac
    16861 cttttggtgc agttgatgag attacacgaa agcaattaca aaaagagata aaagaaattc
    16921 ataaaaaaac aggagttaca attatcttta ttacgcatga tatttatgaa gctttgattt
    16981 tgggaacaaa gactttggta ttaaatcatg gagttgttca acagtatgat actcctgaga
    17041 atattttaaa tactcctgct aaccaatttg tagaccagtt attgaatata agaaaatcta
    17101 taatagatga tgataattta aaagaataaa tttaaattta aaatcaaatt gttgtgataa
    17161 aaactattga tttagagcta acttcaagtt gtatacttga agtggaaaag ttagattaag
    17221 gtatagaagg gcatttagca gttggctgta gacgaggtaa ctgctgaagg tgctttcaat
    17281 aaatggctag aacctgtatc tgttgaagat tataataaat tgaaataaga agctcaatat
    17341 acagagtaga taggcacttc aaatagaagt gtcttttgtg tgccataaaa taaatcaaaa
    17401 tattagcaaa gaatgtgaaa aacaaaataa aataatatgc tatctttaaa ataggaggga
    17461 tgctatatgg ataacaattt gattaccatt gaaaaaactt tagattatat agaaaaacat
    17521 cttttagatg agatgttaga tttaaatgta atttctaaag agataggcta ttcaaaatac
    17581 catttacata gaatgtttac atcagttgtc ggatttagtg tacataatta tatacaaaga
    17641 cgtagactta ctgaagcagc gagaatgcta atttttacag aaaaaccaat tattgagatt
    17701 gctttaatct cggggtatga tacacaacgc tctttttcaa gagggtttaa aaaaatgttt
    17761 aaacgtagtc ctaatttttt tagaagacat caagattttt taccactaca attaaaattt
    17821 gatattcata atagaaaaaa attacgtgga gatatggttt tagatgttaa aaaagttaat
    17881 tgtgcagata taaatattgt tggctattct ggtaaagtaa gaaaaggatt tcatattatt
    17941 ggaaaatgct ggagggattt gcataaaaat aaagagatga tagaaaataa ggtagattta
    18001 ggttttttaa ttggagttag tgattatagc aattttaaag agattgagaa tactcctaca
    18061 tttacataca ttgcaggtgc tcaagtagct acttttggca aattacctaa tggaatggaa
    18121 aaattcgtat taccgaaaag taattatatt gtttttactt ttattgggaa aaatgaggat
    18181 agtatgcaaa atgttgtaga atatatctat caagaatggt tccctaattc aagttgccag
    18241 tttaatgaaa ataatttata tgactttgta aaatatggag aaaaaactga tgagaatggt
    18301 ttgagtgaaa ttcagttttg ggttccaatc atttaaaaga aggtattatg tataaagtag
    18361 atggatttat ataggcaaag ttttacagca gtatgtattt ataaataatt tatagtttgt
    18421 aggaaagaaa taattttact ataagattat gataaaatac acaatactat gacaaaatag
    18481 tgttaaataa tagatttaat tttgcatagg aaaagttcta taatggtgtg tattcataaa
    18541 taatttataa tctgtaggaa agaaatagtt ttactataag attatgataa aatacacaat
    18601 actatgataa aatagtacta aataatagat ttaattttgc aaaggataag gtgttttaat
    18661 aaaaatgaat gtagaaaacg aattacttaa aaacctagat agattgcata caacagagtt
    18721 aggcgttgta agaattaaga aaaatctttc tttggagaca aatgatgtag tgaactggtg
    18781 taaaactaaa atagaatctc caaatgccat tattaataga aagggaaaaa actggtatat
    18841 aagtgtatat gattgtatta taacagtaaa cgctcacagt tatacaatta ttacagctca
    18901 taaagaaaag aaatgaattg caaaatacaa aggtcaaaag cattttccac tgtttgtagt
    18961 acctatgaag aagtatataa aataatatta cttttgaata ataatttaaa ttttggaggt
    19021 tgaaaaaatg aatgatactg taaaagaaaa attggcagag tcactaactt ctgatactac
    19081 tgatatttta ccttatttgc cttatttatt acaagatttt tgggaattag gaagttcacc
    19141 agaacaaatg ttacaattat ttagaaagca tatacctttt aatgaggata caaaagtttt
    19201 ggatttagct tgtggaaaag gtgctgtatc tgtgaagctt gcaaaagagt tgaatataca
    19261 tatcaaaggt attgatttga tacctgaatt tattgattat gctagaagaa aggctattga
    19321 atataaggtt gatgctttgt gtgattttga aatgaatgat gctaatagtt ctgtaaaaat
    19381 tgagcatgat tatgattgtg tgatttttgg agctgtaggg gatattttag gtaattataa
    19441 agaaactata agtaagctat tacctacaat aaaaagtaag gggttcattt taattgatga
    19501 tgcatatgta gtagataaat ctacaaatga acagcttaat tttgagaggg tgtatcccac
    19561 ttacaatgag tggtgtatat tgttcaaaga attaggtctg aagctaattg aatgtatacc
    19621 atgtttagaa cttgaaaatg atgttgaaga ggaaatgaat tggattataa ctcgtgcaaa
    19681 tgagctaatt aaaaaatatc cttctcaaaa agatatgttt gaacaatatg ttattagcca
    19741 aaaaaatgaa tatactgatt tacagcagga ccttgttgga gcaacttgga ttttacaaaa
    19801 agaataagat aaatattaga ctagataatc aatgttatag tgaattatcc catgtctagt
    19861 gaatataaat aacataaaaa accgtcaaga tgaatagttt attcgtgctt gacggttttt
    19921 agtatttgtt gaagtaaaat tttatttctt tagtatatca cttttagtca atccatttga
    19981 tttagcttga gtattccatt gatgattccc cataagttta tataggaatc tagggaagtt
    20041 aggtgaaata aaaatagtac ttttgttgga atcatctata tgtatattat cactaagtat
    20101 attttttgaa agttctttta atgcaattcc taaatctttt ttaggtcctt tacccaaagg
    20161 tacggattct agtgaaccaa acatctcccc agctccaatt ccaatacctt gaacccatgt
    20221 aaagttgatt tttttacacc aatgctctat catttgaatt gccaaatgat tatgttcacc
    20281 ttcattgaat ccacagttaa ctattgcata cactggaata ggttttttat tttgtgtttt
    20341 taaaaatttt tcacatttct ctaaaaaatg aagtaaatga gatggaatac catctacata
    20401 aagaggagat gcgattatta atctatcaca attaagtatg gcattaagag cgtcaggata
    20461 cttagatgtg ttatctactt taaatgtaaa aactttatta tcattttgaa taagttgttt
    20521 taattcagat aaaaaatact gtgaattatt tttatttact tttggactac catttatcat
    20581 tactatattc atatggattc acctcttata tcttgtatat tttcatagaa acaaacagag
    20641 tgttctttta tattgaaatt tatagcattt ccatttacta atttcgtagc agtttctttt
    20701 tcagcttctg taatacaatc tccatagaat atagctttta aatttatctg tttatcatat
    20761 ctgcttttat gacgcatcat attatctttt attacaaagt atggaagaat ataattaatg
    20821 cttttatcaa gtacgttttt gacaaaaggg ctaaaccctc cgtaacagca tctgcttatt
    20881 ataataattc catcagagtc atttataagt ttacccatgt tgctgtaata atcttttaca
    20941 acacattcag aaggagtttt aatccagcaa ccaaaacaac caatacattt gtggatttct
    21001 tttgtgtttg gaagtatttt tatattgtca tcaagtttgg gtaataactt ttctaattct
    21061 ttgtcagtta aatcatgtat taaaagcctc ataaagtcca tctcctttta ttatgtatgt
    21121 ttacttctaa aaattatatc ttatagattt ttcagtaaat ctttacacca attaataaca
    21181 aaattataat agtcaactcc ataaatcatt gttgcataca tatatgtata ttggtcaaca
    21241 caatgatttt cttctgcatc atttttttgt ttacttaatt tatttagaag taattctact
    21301 tcagttatta acatttttaa gtttgtttca attatttctt tgggtagata cttgtaaaag
    21361 aatatattaa caaggtggtc ttgctttgtt ttagaaaaat ttataggttt ctttaaccat
    21421 tcaagaaata cttctttacc agtattagtt atggaatata gtttttttaa tttgttacca
    21481 tctataactt cgtgatagtg tatatatcct tttgtttcta atcttttaag tgcaggatag
    21541 atacttccaa agctggcgtc aaaaaagtac gaggtacttt ctgacattat ttgttttaag
    21601 tcgtatccac ttaattcttt ttccattaaa aatcctaaaa taatgtattc taacatatta
    21661 cctcctggta tcaattagat atatatcgat tcgatatatt tattattaca gataataaat
    21721 cttacgtcaa gatgcttttt aagataagct aaaaagaatt aggagttatt gataaatatg
    21781 caaaaaaatt aatataaaag gtaattaaat atcaataaag atttaaaaat agttaaatca
    21841 aagtaagagt ctaatatata aaatttatag taactttatg gtttaaatat aaacttgtgt
    21901 gctattgaca aagtataaaa ttagcagtag aataattgca tgagcaaata gattgttgcg
    21961 aacgcaaaaa aaggacaata gatattaaag gagagaatat gagagagcta ttaaaatggg
    22021 tttccattat aaatagatac aatacaaatt atattgacag gaaattggcg gatattggaa
    22081 tcaataatag ccaatatttt tatgtattac acatatgtaa taatccaggt attacacagg
    22141 acactatatt tgagaaaatt cttgtgaatc ctagtaatat aacaagagct ttggcaaatt
    22201 tagaaaaaga aggctttata acaagagaac caggtgtaaa ggataaaaga acatggcatt
    22261 tatatcctac agaaaaatct ttaggatgtt atgatgaaat actaaaaata acaaatgggt
    22321 atgttgagga gttgttgagt tcatttgaga aagaagagca agagttgttt atgtcaatgt
    22381 taaagaaagt tgcatttttg gctattgata tgaatgaaga agcaaaattg gaggcgaagg
    22441 aaaatggaag aaaaatataa ggagccattg gaatatgaaa aggtttcaaa gttattaact
    22501 aagtacgcaa ttcctagtat tatagcaatg ttagtaagtg ctttatataa cattgttgac
    22561 caaatattta ttgggcaagg tgttggtgta ttgggcaatg ctgctacaaa tgtcactttt
    22621 ccactaacaa caatatgtac tgcaattgca ttattgttag gaattggagg agcgtctaag
    22681 tgttcattgg aattaggtgc taaaaattca gaaaaagcta caaaagcagc aggaaattct
    22741 atatgcttga tggctgtttt tggaataagt ttatttatag tagtatcaat atttttaaca
    22801 ccactattaa aattctttgg ctctacatca gaaatactac cttatgcaca cacatataca
    22861 aaaattacat caattggatt accatttctt ataatgtcca ctggaatgag taagttgata
    22921 cttgcagatg ggagaccgaa agcttctatg ttttgtatgt taataggagc agttattaat
    22981 acaattttga atccattatt catttttgtt tttaatatgg gaattgctgg gtctgcttta
    23041 gcaactgtta ttgggcaact tatatctttt ggaataagtg ttcattatat aaagaatttt
    23101 caacatatta aattacataa gaaaagtttt aaattagata gtataattta taaacatata
    23161 tttgcgctag gttcaagctc atgttttaat caattagcaa tgactgtaac acagatagtt
    23221 atgaataata cattggcata ttatggggct aggtcagttt atggaagtga gattccactt
    23281 gcttgtgtcg gtatcattat taaagtaaat atgattttta tatctataat tattggtata
    23341 tcacaaggta tgcaaccaat tatagggtat aattatgggg ctgaaaagta taatcgtgta
    23401 aaagaagcat ataagtttgc agtgggaagt gcaactatta tatctgttat tgcattctta
    23461 tgtttccaat tattcccaag acaaattacg tctatatttg gtaacggaaa tgagacttat
    23521 tttatatttg cagaacatta ttttagaact ttcttgttct taacatttat aaatggtgtt
    23581 caaatagtat catcaacttt ctttacatca attgggaaat cagtgttggg actttttact
    23641 tctttgactc gtcaaatttt atttttaatt ccattgatag taattttacc aatgtttatg
    23701 ggaattgatg gagtaatgtt tgcaggacca atagcagatg cagcagcggc aatggtatgt
    23761 atatactttg cagttcatga aatgaaatca ttgactataa agcaaaataa tttgatgagt
    23821 aaccataata agattgcaga ggtataagac aatatttaag aagtatagaa aattaattta
    23881 aagaagtata ataaactaat ttaggaagta tagaaaatta atctaaagaa gtataataaa
    23941 ctaatttaga aagtatagaa aattaatcta aagaagtgta ataaactaat ttaggaagta
    24001 tagaaaatta atctaaagaa gtgtaataaa ctaatttagg aagtatagaa aattaattta
    24061 aagaagtata ataaactaat ttaggaagta tagaaaatta atttaaagaa gtataataaa
    24121 ctaattcagg aagtatagaa agctaatcta aagaagtata gtaaactaat ttaggaagta
    24181 tagaaaatta atctaaagaa gtataataaa ctaatttaag aaatatagaa aattaattta
    24241 aagaaataca atacattaat ttttaagtaa cattaaatta atgtaagtaa aataatgttc
    24301 atatgaagat ttaaaggtgc attttctgaa gctaagtata tgttgtagag tgttaggtct
    24361 ctctatcctg tgaagtttaa tagctcaaga aaatgcacta tttatgatga ttattttgtt
    24421 ttatttccaa ttttggaaat aactattcta attaaatcta aaattatacc ataaataaga
    24481 aaagaaagta tagatgctat gtaccaataa atcgaaattg accctgtaat tgttctacat
    24541 gggatttgca tacctgaatc cataaaaaca aaaaaactat caactctgga aataatagac
    24601 aaaataggat ttaaacctat tagaacaata ctttttgagt cttgaccaat ttgatgcata
    24661 taaatctcaa ataatgcaat taaaaaaaac caaaatgtaa aagatttaat aatttttttc
    24721 atcaaaatac ctcacataat ataaattctg tttgcactat ttgcttacta caactattta
    24781 tctccattca gtcacatcac ttaattgtaa tctaggtctt cttgatggtt cttcatcagg
    24841 ataacccaaa gatattgctg ccactaattg actatctgta tttaaccatt cacataattc
    24901 acgatatgca aagtatacat cacaaatcca aagactacct agacctaatt ccacagctgt
    24961 aagcgacata ttctgaattg cagctccaat tgattgcatg tttgccattt catagaatcg
    25021 ttcttcaggg gatagctttt ctaaaggtga tttacctaat atatttaaaa taaaaattgt
    25081 tactggagct tgtttcatga tttcaactgt gtaatttgca ccagcgatat gttgacgact
    25141 tcctggaaga agcccattat cattaatttc attttgaata cctttgctca tggctttaag
    25201 catagattct ttttcctttt cagtgatgac aacaaaacgc catggttgcc tgtttttaga
    25261 tgaaggtgca tttattccag cttcaattat tttctctata gtttcatgtg aaatgctttg
    25321 atttttatat tttctgatac ttcgtctttt tgatatagaa tcacttatca ttttaagtaa
    25381 cctccgttta ttttttggaa aaatctattt aatttttatt ttctatttta attattttag
    25441 cattaatcag aaaaaaagtc attgatttta gtatacaatt attaatgatt atattaagaa
    25501 ttgtatcaaa aagtatgtga ttatactaag ggattatatt aaacattata tttaaaattg
    25561 tatgaaaggt tgtatttaat attatattga gagatatata tattaggatt tatatatatt
    25621 cagatttatt attatattga gccttatatt aaattaagga ctataatttg aaaataagct
    25681 ataaaaactt aatattgttt tgttcagaga taaaaaaaga atactttagg tgatttttat
    25741 taaaaattaa gagtgataaa
//
LOCUS       DAMJVI010000047        24788 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.049, whole genome shotgun sequence.
ACCESSION   DAMJVI010000047 DAMJVI010000000
VERSION     DAMJVI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24788
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.049"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..378
                     /locus_tag="PC545_002032"
     CDS             <1..378
                     /locus_tag="PC545_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2456799.1"
                     /translation="AIFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECESIVKEV
                     KKVATKSLGGHGSKAYKNKSLRGKVYNDIYHQIKREFGVDSYKAIKRCQLNKVLEIVN
                     NYKLPIVFEEEIRLLNSQLSIVS"
     gene            481..969
                     /locus_tag="PC545_002033"
     CDS             481..969
                     /locus_tag="PC545_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456800.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAVGYDEKVSPTYAFNSTVENEIIKKERDITRLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            1716..1889
                     /locus_tag="PC545_002034"
     CDS             1716..1889
                     /locus_tag="PC545_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456801.1"
                     /translation="MATKSILKNVDVRKKAFGRNLVSALENAKNKQEKEVVLSKKCSE
                     VPKDKIKDIFGRF"
     gene            1889..2500
                     /locus_tag="PC545_002035"
     CDS             1889..2500
                     /locus_tag="PC545_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDF2456802.1"
                     /translation="MSGYLIVNLSNMLGELEEEEVKKILSSFSCPLNKDVEEFLKNKA
                     IEFSKQGLASTHLVLTSYKGKPVIVGYFTLANKYFTIKRKTLSNSLARKIVKFGQYNE
                     ELRRYIIGAPLIGQIGKNYSNNYNKLIKGDELLKIACDKIKAVQLDMGGKIVYLECED
                     KPKLIEFYKDNGFVDFGKRSLDKDETDSLDGDYLVQMLKYLKK"
     gene            2577..2765
                     /locus_tag="PC545_002036"
     CDS             2577..2765
                     /locus_tag="PC545_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456803.1"
                     /translation="MEIKKNTQDVVSERKNSLDSEFKIPASGVCYMAEFIKESREIIK
                     ELDKHFESCLDVLSKARL"
     gene            complement(2745..3062)
                     /locus_tag="PC545_002037"
     CDS             complement(2745..3062)
                     /locus_tag="PC545_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456804.1"
                     /translation="MSKNIDIRNLKQFQKKIERMEQNLKPDFDKIRTIILSKKFMNQH
                     TNFDSFDELLAFGNYIVNSEEDFLAIPDNEFDLFIVKNTDFPDWQTMLDSAYSKYLES
                     CLR"
     gene            3186..3413
                     /locus_tag="PC545_002038"
     CDS             3186..3413
                     /locus_tag="PC545_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456805.1"
                     /translation="MGFEISGNLNFDSVIDDLKKEVENNPTIFTSQNVGNKFKEKCKI
                     CEKISEFEILEDGKVKCLECGTEFELNLKVE"
     gene            3483..4175
                     /locus_tag="PC545_002039"
     CDS             3483..4175
                     /locus_tag="PC545_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456806.1"
                     /translation="MQDVKEKVKQDYLKGMKQKEISSKYDISLNTLKSWIKRYNWASE
                     KKKGAPINKRGAPFSNKNSVGHGAPKENKNAEKFGFFSKYLPEETRELIQEISIKDKF
                     DILWEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQAS
                     FLNAQSRAMSELRSLIKQYDEMIHKDWNLATEEQKTRVEKLKCEVDNLSKDDIGDDEL
                     KISVDYGDRNDS"
     gene            4153..5406
                     /locus_tag="PC545_002040"
     CDS             4153..5406
                     /locus_tag="PC545_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HDF2456807.1"
                     /translation="MVIEMIVRVNFNPDFKEANFTKKRYRAMKGSAGSGKSVNVAQDY
                     ILKLGDKKYQGANLLVVRKSEATHKYSTYAELTGAINRIYGKQADKYWKTTLNPLEIK
                     SKVTGNSIIFRGVNDAKQREKLKSINFSKGKLTWVWCEEATELMESDIDILDDRLRGI
                     LTNPNLYYQMTFTFNPVSATHWIKRKYFDYKNDDIFTHHSTYLQNRFIDEAYYRRMQM
                     RKEQDPEGYKVYGLGEWGETGGAILKNYVIHEFTTEFEYFDNMRLSQDFGFNHANVVL
                     RIGFKDGELYICNEIYVHEMDTSEIIKIANSIGLEKTLFMYCDSAEPDRIKMWKNAGY
                     KAKGVKKGPGSVKAQIDYLKQLRIHVHPSCTNTIKEIQQWKWKQDERTGLYLDEPVEF
                     MDDAMAALRYSIDNKLKNNGISFLK"
     gene            5412..6851
                     /locus_tag="PC545_002041"
     CDS             5412..6851
                     /locus_tag="PC545_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2456808.1"
                     /translation="MLNIYISETDLIKVQLKKESTFNLVKVIEHYILKHRPEKYKQGE
                     EYYYGNTDVNNKRRYYLLDGAKVDDFTKVNNKAINNYHKLLVDQKVGYSVGNPIVFNA
                     DDDNLTKLLNDLLGEEFDDTITELYLNASNKGVEWLHPYINRKGEFKYVIIPAEEAIP
                     IWDSKRQRELVAFIRFYYIEDIDGNKIKRVEYYTENDVTYFVERGNSFVQEFLYDEYG
                     KMTDIQEGHFRINNKEQGWGKVPFISFKNNEKCVSDLTFYKSLIDIYDNNISTLADNL
                     DEIQEVIYVLKEYPGTSLQEFIDNIRYYKSIKVDGGGGVDKLEINIPVEAKKELLDRL
                     EKNIIIFGQGVNPESQNTGDKSGVALKFLYSLLDLKCSKTEKKFKKAIRELLWFVCEY
                     LKISGSKSYDYKTVQITFNHSMIINEAEKIDMAAKSTGIVSDETIVSNHPWVEDVNDE
                     LERLKKQEDTQKEYDDLIPNNQDGVIDET"
     gene            6841..8238
                     /locus_tag="PC545_002042"
     CDS             6841..8238
                     /locus_tag="PC545_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDF2456809.1"
                     /translation="MKHKDYWRKRFEQLEEAQNNKSVKYYLELEKQYKLAMNSIEKDI
                     LAWYNRFAKNEGISLLEAKKLLNTRELEEFKWSVEEYIRHGKENAINQKWMKELENAS
                     ARVHITRLEALKLQIQQQVEVLYGNELDGIDKLMRHIYTSGYYHAAFNVQQGVNVGWS
                     LMNLDTNRINKVISKPWTSDGLNFSERIWGKHRPALINELHTKLTQSIIRGENPKNLV
                     NDFSKRFNVSKSQAKNLIMTESAFFASASRKDCFNDLDVEKYEIIATLDLKTSNICRE
                     LDGKVFDMKDYQVGVTAPPFHCRCRTTTAPWFEDEEGYRAAKGEDGKTYYVPSSMKYN
                     EWYEKYVKHNSILEIKNSAIIDSIKEDIKNGKYNLNIHDGKQGKHLKEHNNYIEGRSY
                     LTITKEEAQELVNKHAGNGIIKFNRSGEWDKKELIEVDKNIGVNVNNITGEKTLTNKF
                     KIHYSKTGTHIVPAL"
     gene            8253..8453
                     /locus_tag="PC545_002043"
     CDS             8253..8453
                     /locus_tag="PC545_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456810.1"
                     /translation="MKLWEYVGKNVQITCVDKQIIRGKCDGYTQALDNEPEIASISIA
                     RDGYGIEVYENEIESIENINKE"
     gene            8519..9082
                     /locus_tag="PC545_002044"
     CDS             8519..9082
                     /locus_tag="PC545_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HDF2456811.1"
                     /translation="MDWLKELLEGIKIEDNKIDVASLQKSIEKKIKETTITQEDYTNL
                     ETQLNTANETIKKFEGGMTKEDVENLKTTYETDKKTLEETYKKEIEEKDFNYWLNDAF
                     KSIKCRDEIALKAHLDIEALRNSKDRQKAFEEQINPLKQDKDYLFNATLEGEEPKIDT
                     ITPGQEPKINDFGFNFTGVRPHENNNK"
     gene            9094..9993
                     /locus_tag="PC545_002045"
     CDS             9094..9993
                     /locus_tag="PC545_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
                     /protein_id="HDF2456812.1"
                     /translation="MAALNYAKEYSNVLAQAYPYTLNFGDLYATPNNGRYRWTGSKTI
                     EIPTISTTGRVDSNRDTIAVAQRNYDNAWEPKVLTNQRKWSTLVHPADINQTNYVASI
                     GNITKVYNEEQKFPEMDAYCISKIYADWTALGNTADTTVLTTTNVLEVFDKLMEKMTE
                     ARVPENGRILYVTPVVNTLIKNAKEIQRTVNIKDAGTSLNRQTTDIDTVKIIKVPSNL
                     MKTAYDFTTGWKVGAGAKQIFMSLVHPSAIITPVSYQFSKLDEPTAVTEGKFFYFEES
                     FEDVFILNKKADAIQFVVEGAGA"
     gene            9993..10226
                     /locus_tag="PC545_002046"
     CDS             9993..10226
                     /locus_tag="PC545_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456813.1"
                     /translation="MAQVRKLNRILTIEECKIDDFLEMGYDLIDETGKVVRYGKSLNV
                     KDLIAENNILRSKVESLEEENKQLKEKNKLTKK"
     gene            10236..10637
                     /locus_tag="PC545_002047"
     CDS             10236..10637
                     /locus_tag="PC545_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456814.1"
                     /translation="MGNNIIDDIEKRLESFGYILKDGDKWLIDFVREKIENIIKLDCN
                     IKTMPIELKEIEADMIVGEFLFTKKNMGQLDIESINFEAVEKSISEGDTKVDFAIGSG
                     SQTPEQRFDSLIAYLTAYGKNKILTFRCLRW"
     gene            10631..10978
                     /locus_tag="PC545_002048"
     CDS             10631..10978
                     /locus_tag="PC545_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456815.1"
                     /translation="MVSKTRKAIEMLYRYKCTIVEYQPIKDPVTKRTNNKEVIVLENQ
                     PCKLSYKNIVSATEGKLAKLEQTIKLFISPDIEIKAGSKLIINDKEYVRSGESAIYPN
                     HQEIILELFKDKA"
     gene            10978..11403
                     /locus_tag="PC545_002049"
     CDS             10978..11403
                     /locus_tag="PC545_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDF2456816.1"
                     /translation="MARWGSVDFREFKRVCKKMEKLTKIDLDKFCKDAARELAARLLG
                     KVIRRTPVDTGFLRQGWNGVAYARSLPVYKQGNNYIIEVVNPTEYASYVNFGHRTKDG
                     KGWVKGQHFLTISEMELQSQVDKIIEKKLLILLKGVFDA"
     gene            11396..11833
                     /locus_tag="PC545_002050"
     CDS             11396..11833
                     /locus_tag="PC545_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456817.1"
                     /translation="MLNNIIDGISVKLDKSFGEKYTIYSEDVEQGINEPCFFIVPLNP
                     SKTPYPSGRELKKNSFDVHYFPRSEAKNFEINEIAEMLLEELEYIEINGDLVRGTNMN
                     FEIIDNVLHFFVDYNYFTIKSNNADKMNTVELFGGLKRGDNFE"
     gene            11826..12002
                     /locus_tag="PC545_002051"
     CDS             11826..12002
                     /locus_tag="PC545_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456818.1"
                     /translation="MSKTLSKGTDYKFTKEQIVNSKKYVNRKDLLNAILKENELYSFS
                     EVEDRINKFMKGVS"
     gene            12003..13313
                     /locus_tag="PC545_002052"
     CDS             12003..13313
                     /locus_tag="PC545_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HDF2456819.1"
                     /translation="MALGGGTFVTQNKVLPGSYINFVSATRATSSLSDRGIVAMPLEL
                     DWGIDEEVFQVTSDDFEKYSTKYFGYDYTHEKLKGLRDLFKNIRLGYFYKLNKGVKAS
                     CSIATARCSGIRGNDLKVIVTTNIDDNAKFDVVTLLDNKKVDTQIAKVITELQDNDYV
                     TWKKEATLEATAGLTFTNGTNGEAVTGTEYQAFLDKIESYSFNALGCLATTAEIKSLF
                     VEFTKRMRDKVGAKFQTVLYKKNDADYEGVVSVENKIKDTGLLESSLIYWTTGAIAGC
                     DINKSNTNKRYDGEFDVDVNYTQIHLEEALKTGKFIFHKVGDEVHVLEDINTFVSFTD
                     EKNDDFSSNQSVRVLDQIANDIATLFNTKYLGEVPNDKSGRISFWNDVVKHHEQLQNM
                     RAIEDFKADDVSVEPGSDKKTVVVSDAVKVISAMSKLYMTVSVS"
     gene            13335..13805
                     /locus_tag="PC545_002053"
     CDS             13335..13805
                     /locus_tag="PC545_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HDF2456820.1"
                     /translation="MAQQIKARDTISASKAECFVTIKGKRYNFMQAINLEAKMEKNKS
                     EIPILGSTTKGNKSTGSKYSGNATFYYNTSIFRELLYEYKETGEDIYFDIQITNEDPT
                     SSVGRQTIILEDCNMDSGIIAKFDADGEYLDEDMDFTFENWKLVEKFNIANGME"
     gene            13858..14115
                     /locus_tag="PC545_002054"
     CDS             13858..14115
                     /locus_tag="PC545_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456821.1"
                     /translation="MIKIKDKYEIKDSISFDYSNKRPLEERVSEMYKKAGKYLIDISD
                     KLATDTIDGSSLKPIIIKFEINEAGVATIEKQTKYLVMEVE"
     gene            14120..14560
                     /locus_tag="PC545_002055"
     CDS             14120..14560
                     /locus_tag="PC545_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2456822.1"
                     /translation="MGDLNAFLSQNAIKVENRKYVASERFIGEDGKAIEWELKAIDSD
                     RDRQLRKDSTIRVPVLNKKGKATGQYTSETDFNTYTLKLCVETVVFPDLHDAELQNSY
                     GVMGAEELLTTMLTPGEYTDLSSEVGEVNGFDRTFEDKVEEAKN"
     gene            14881..15042
                     /locus_tag="PC545_002056"
     CDS             14881..15042
                     /locus_tag="PC545_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456823.1"
                     /translation="MDNFLLSILASLIASLIGYIVCRCIKNVKSHSTRGKSKSGWELG
                     FKIKFRKFK"
     gene            15191..15424
                     /locus_tag="PC545_002057"
     CDS             15191..15424
                     /locus_tag="PC545_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456824.1"
                     /translation="MNKDIELTEKDLYCIARHIQVYVFKKDDEVIKKEDNPCCRCKHK
                     FKYYENSVCIYHCSVFQKLSKITGLKMGFGVKL"
     gene            15484..16011
                     /locus_tag="PC545_002058"
     CDS             15484..16011
                     /locus_tag="PC545_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDF2456825.1"
                     /translation="MREGKRRRGCLFWFIIIIIFSGVVGAIAGNSTNNGSTEKQKEDL
                     TKYIGEEGNIGDLKLTVNSISKASEIPVASGYLAYTPDSGKYGIINLTIKNQTKESQS
                     FMLNSFTLIGPDNSKYVPSLLIDVGNKYITMDTVNPNLDVTGNIAFEIPKDLLVSDCK
                     LKYSGSEQEFILKEK"
     gene            16153..16713
                     /locus_tag="PC545_002059"
     CDS             16153..16713
                     /locus_tag="PC545_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2456826.1"
                     /translation="MFCSNCGAEITGIGKFCSSCGVAVETEIIEDNNIKSNDLIVDAN
                     GIEINMTEIYRKYRKEKVNAIKNVMEISGLNIKEAKKIVDFSFEELKINFIDDTMSNS
                     EKEKIIQNQNRKNNIEKAQQESVACCPKCGSTSLTAQKKGFGIGKAMVGASLTGGIGL
                     VAGNLGAKKVRVTCLNCGKQFWAGKK"
     gene            16791..19079
                     /locus_tag="PC545_002060"
     CDS             16791..19079
                     /locus_tag="PC545_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDF2456827.1"
                     /translation="MATIQTSIRIFDGMTPAFRHMTTAMNIVLSSFEQLQRTSSNAVN
                     ANSIIRAREELARAEAGFDRLERQIRESDNQQRKLNEDINKGASSTDRLVGSAKKLAA
                     TYLGIRTLGGLGNLSDQMTSTNARLGMINDGQQSDAGLNKMIFQSAERSRASYLDTAQ
                     IVSRIGMNAGKAFSSTKEIVGFAEQLNKKFVIAGASTEEMNSALLQLTQGLSSGVLRG
                     EELNAVFESAPNIIQSIADYLDVDIGKIRGMASEGMLTADIVKNSLLAAAEQTNAEFE
                     KMPYTFSQIWTSIKNNAIMIFGVIQKKIEQSMSSKGFRTFIDNFINSLYVLGNVAYNI
                     FNEIISILGSPFFQAFVNAIIVGVSLIVQALGWIITQALNIANVFAQNWSIIAPIVLG
                     VAAAMLVYNNALLLSIANKVKDIALSAKSLVMSFAHIVAESYRAAALVATTIAQDGLN
                     AAMAACPITWILYGIIAIVVAFFVAIAIFNHFAGTSVSAIGVVAGAISVAASFIGNLF
                     IATGNLIIDIVALIYNTLAGFAEFFANFLDDPIGSVIRAVSGMANAVLGIIRSIASAF
                     DTVFGSNLADAVSGWQDKLQGWTDKVAGEAKIKVERMDPNKLHFDRFNYGKAWDAGYK
                     WGDKLETNIKDKFDISKMAEDAKKKLGLDDLWDKKYGLGDGFGSAGLNSPLNDAAKGA
                     KDTAGNTAKMAKTMDKSQEDLKYLRDIAEQETINRFTGVNIKIDMNNTNNISKDNDLD
                     GIVNVLTEKLNDAMAVSAEGIV"
     gene            19096..19797
                     /locus_tag="PC545_002061"
     CDS             19096..19797
                     /locus_tag="PC545_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2456828.1"
                     /translation="MAYDFYLDGVQLPIAPPKLEVKVTNKNKTVDLINVGEVNILKKE
                     GLSEISFEAEFTHNKLPFYRGTFRDVQFFLSKLELLKTDCKPFQFIVSRELGNKVLFN
                     TNIKVSLEEYAISEDADNGSDTKVAIKLKQYRDYSTKKLVPATPEKTNYGRTPPPVMK
                     PKEFRPDSSNKPNGKTYTVKAGDSLWAICQKQLGNGSLYKKVYELNKTMMDKANKGKK
                     LSKYTIYKGQVLRLV"
     gene            19790..21760
                     /locus_tag="PC545_002062"
     CDS             19790..21760
                     /locus_tag="PC545_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2456829.1"
                     /translation="MSDDLVLANDRDIRLVIAHWEDFYEPVVLDGITWEIERRGTPSK
                     LEFTIVMDDILEFCEGNSVRLYYKGVGIFYGYIFQKKRDKENHIKIVAYDQLRYFKNK
                     DTYVYSNKTASELVKMLAKDFKLKYNVIEDTKYKISRVEENKTLFDMVLTALDDTLRE
                     KKEMYVLYDDFGRITLKNVASMKLDTVMNNDVIEDFDYNSSIDSDTYTKIKLVRDNEE
                     TGKRDVYIAQDSTHMRSWGILQLFDTVDKNMSEAEIKQKCDILLKLYNKKTKSLSLKN
                     VLGDIRVRAGCLVPVFLSLGDIDLQNYMLVEKVKHTFENNSHFMDLTLVDGDEFASYS
                     SSSYSSGNTNNKNEKQNGPAQSTTSKEDTDMANKINKLLKGKLSNTGNIFVKYSNAYK
                     VNPALMAAISMHESARGTSNIANTKNNFFGMKKNGDYMSFSSVDEGIKRGISNLSRNY
                     IHIGRKTLESIRNKYSSSSDKEWVKCVGAFYKQITGSTYNSNSAGTGVGSNEEAEKNL
                     KDLTYQVQNNNSNTSTNNNNKVSKVIQEAKNQLGKPYKWGGNGPKSFDCSGLMVWAFK
                     RGAGINLKRVSADQSKDSRGKLLCNINDVKAGDLVFFAYNKGKGNVHHVGLYIGNDQY
                     IHAPQTGDVVKISSLSGRQKKKHDFARARRFF"
     gene            21774..22034
                     /locus_tag="PC545_002063"
     CDS             21774..22034
                     /locus_tag="PC545_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HDF2456830.1"
                     /translation="MSQELLQIIKKAAMDAVETSNPMQIAFGTIESVNPLIVKIEQKA
                     SFEEFFLIQTETFKRYTDKKIGDKLVLIRMQGGQQYLILDRM"
     gene            22039..22458
                     /locus_tag="PC545_002064"
     CDS             22039..22458
                     /locus_tag="PC545_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2456831.1"
                     /translation="MLPTDNIDYDIEDVSIINFDVRQEPSKTFKLNIEKNRVDGICDD
                     VEALKQTIFLILNTERYEHLIYSRNYGVELNDLIGEPISYVIPELERRITEALIQDDR
                     IENIDNFEFQNIKGKVQCRFSVHTKYGNIKAEKVVSV"
     gene            22459..23508
                     /locus_tag="PC545_002065"
     CDS             22459..23508
                     /locus_tag="PC545_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2456832.1"
                     /translation="MFELMTFENIIKRMLDSVPDTFDKREGSIIYNALAPVAIELTET
                     YIAMDELLDQTFVDTASYYYLEKRCKERGITPLEATNTIAKGVFNIDIPIDSRFNLGE
                     YNYVAIERISEGIYKMKCETAGPIFELGQLIPIEYIDKLETAELTEILINGEDEESED
                     SLRQRYYDSLNSQSFGGNMQNYKDEVNKIQDVGGVKVYPVWDGGGTVKLVIINSNFKV
                     PSEDLVNLVQEEIDPIGHQGQGLGLAPIGHKVTVTGVVSTTINISAEITYKNGYTWEN
                     IKSIAEEAIDDYLNELNMSWEDEENLIVRISQIETRLLSIDGVLDIANTMINEVKSNL
                     TINSNSIVVRGEVVG"
     gene            23501..24118
                     /locus_tag="PC545_002066"
     CDS             23501..24118
                     /locus_tag="PC545_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2456833.1"
                     /translation="MDKEINLINYLPQILQDKEEYIKVFNVGNKEIKILHDKLKDLSN
                     DQFLEDLTISGIKRWEKIMSITPKSNESLEDRRFRIFSKYISKLPYSERFLRNWLDSI
                     VGEGNYELTINNATYNIHLESDARNQDWFEEVHSFVSNIKPCNMTLDYTRVLISKDNY
                     MNFGITTLMGQEITIYPWSPPDIETYGEIDVLTGNGVGYQEITIF"
     gene            24130..>24788
                     /locus_tag="PC545_002067"
     CDS             24130..>24788
                     /locus_tag="PC545_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2456834.1"
                     /translation="MAIDKSYYTIITDVGKAKIANASVTGNKVGFVKIQLGDGGGSEY
                     TPTESQTALKNVVWEGNIGNTTTDETAPNCIILESLIPSSVGGFMIREIGYLDDENNL
                     IAISKYKECYKPSIEQGAVVDMKVKTVLIVSNVNNIELKIDPTIIFATLKDIQDLETK
                     IGTVNTKIDTTKTELTNNLETAKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQTIT
                     I"
BASE COUNT        10090 a         2694 c         4754 g         7250 t
ORIGIN      
        1 gctatattta tgctagataa gaaacaagaa gtcttagaaa ctaaaataga gaatgttaat
       61 gagaaattag agaactttat ggatgatgca ccactattca atattgaatg tgaaagtatt
      121 gtaaaagaag ttaagaaggt agcaacaaaa tcactaggag gtcatggaag taaggcttat
      181 aaaaataaat ctttaagagg taaagtatac aatgatatat accatcagat taaacgagag
      241 tttggagtag atagttataa ggctataaag cgttgtcaat taaataaagt attagagatt
      301 gtaaacaatt ataagttacc tatagtgttt gaagaagaaa taagactttt aaatagtcaa
      361 ttatcaatag taagttaaat ttattcaaaa aaaaggaaaa ggagtgcttt cacactccac
      421 ttgtcaaaaa tataaaactt ttatccaaga ttattataac ataaacagga gtgtggaagt
      481 atggataata atatcaataa aaaagaacta tttaaaaaag tagaaggtag attacatcat
      541 tataaatttt taagtgcaga aattaagaat cttgaattag atatagaaag tagagaaaat
      601 gagatatttg ggtgtaaggc tgttggatat gatgaaaaag taagtccaac atatgctttt
      661 aattcaactg ttgagaatga gattataaaa aaagaaagag atattactag attgaaaaaa
      721 ctgaaaaaag ataaagaaat agaaaagaag aaaatagaaa atgcacttac atgcttagat
      781 ataagagaag aacatttttt taaactgttt tataatagta gaatgaaaaa tagtatggtt
      841 tatatatcct tagagatgaa ctcagatagg aaaacatgta gatgtgtaag ggaaagatta
      901 gtgtataaag ttatggatat gctttatcca agaattaagg aaaatgaact cccattattt
      961 aaaaattaga aaattcccca gtttttcccc agaaattccc actttattcc ctactttctc
     1021 ccctttttga ttaaaaaagc atgagataat agtattgtgg aaataaagat ttccctctca
     1081 aaacttaata tttgactagg gtataaggga ttgccctagt cactacgaac agactaggca
     1141 gggcgtgagg acgctgttag ttcaattcta actatgttca aatattaatc aacgtataca
     1201 ctaaaagtag agaaattgag ggcaaaattt tatattttgt atcttaattc agaagtctaa
     1261 aaatcgggtg gggcttggta acctcactca ccatgcaggt actggtgtct agtctaagtt
     1321 cgattcttag aacctgcgac ataatatatg tatctcccta ctaaaaatgc taagtttact
     1381 ccaaacttag cattttaatt tttaaaaaga aaaaagaaat ttttattgtc ataatactat
     1441 ttgtttaggt atattataat gtgcatactt aaaattaaat acttagcaag catttgaatt
     1501 aatatacata agatatatga cataattttt tatagtgtaa gttatttaaa ttaattataa
     1561 ataacagtaa ttttattata taaaatgtac atattgtgaa taataataat aaaatcatgt
     1621 acaaaatgcc aactgataat tcctcgaaat atattgcata tttaacgtaa cgtcagtata
     1681 attaaattat aatagtgaag tggaaggtgg tacttatggc tacaaaaagt attttaaaaa
     1741 atgtagatgt aagaaaaaag gcatttggaa gaaatctagt atcagctcta gaaaatgcta
     1801 aaaataaaca agaaaaagaa gttgtattaa gtaaaaaatg ttcagaagta ccaaaagata
     1861 aaataaagga tatattcggg agattttaat gagtggctat ttaattgtaa acttaagtaa
     1921 tatgctagga gagctggagg aagaagaagt taaaaaaatt ctctccagtt tttcttgtcc
     1981 ccttaataaa gatgtagaag aatttttaaa aaacaaagct attgaatttt ctaaacaggg
     2041 tttggctagt acacatttag tgctaacttc ttataaaggc aagcctgtta tagttggata
     2101 tttcactcta gctaataagt attttacaat aaaaagaaaa acattatcaa actctttagc
     2161 taggaaaata gtgaagtttg gacaatacaa tgaagaacta agaagatata ttattggagc
     2221 acctttgata gggcaaatag gaaagaatta ttcaaataat tataataaat taatcaaggg
     2281 tgatgaactt ctaaaaattg catgtgacaa gataaaagca gtacagttag atatgggtgg
     2341 gaaaatagtg taccttgaat gtgaagataa acctaaatta attgaatttt ataaggataa
     2401 tggatttgta gactttggaa aaagaagcct tgataaagac gaaacagatt cgttagatgg
     2461 ggactattta gttcaaatgt tgaaatatct aaaaaaataa aagtacataa aatctaaaat
     2521 gactatcttg atagatggtc tttttttata caataaatta aaaggagaat gaaattatgg
     2581 agattaagaa aaacacacaa gatgtagtat cagaaagaaa aaattcccta gattctgaat
     2641 tcaagatacc tgcaagtggt gtgtgttata tggctgagtt tataaaggaa tctagggaaa
     2701 tcataaaaga attagataaa cattttgaaa gttgtctaga tgttttatct aaggcaagac
     2761 tctaaatatt ttgaatatgc tgaatcaagc atggtttgcc aatctgggaa atcagtattt
     2821 ttaactataa ataaatcaaa ttcattatca ggaatagcta aaaagtcttc ttctgaattg
     2881 actatgtaat taccaaatgc aagtagttca tcaaaagaat caaaattagt gtgttggttc
     2941 ataaatttct tgctaagaat tattgtgcga atcttatcaa agtcaggttt taaattttgt
     3001 tccattcttt caattttctt ttgaaattgc tttaaatttc taatgtcaat atttttactc
     3061 ataagaacac ctcctttcaa tagaatatta gcataaaatt gtggtgaatt ctgctattgt
     3121 cgaacgattg ttgaaggata ttgtataata acatagaatt tactatacta taaggaggtg
     3181 attatgtggg atttgagata agtggtaatt tgaattttga cagtgttatt gatgatttaa
     3241 aaaaagaagt tgaaaataac cctactatat ttacatcaca aaatgttgga aataagttca
     3301 aagaaaaatg taaaatatgc gaaaaaatat ctgaatttga aatactagaa gatggtaaag
     3361 ttaaatgttt agaatgtggg actgaatttg aattgaatct taaagtagag taaacaaaaa
     3421 aagaatctca attatgaggt tcttttttta ttcccaaaac aaacaaataa agaggtggtg
     3481 atgtgcaaga tgtcaaagaa aaggtaaaac aagattactt aaaaggaatg aaacaaaagg
     3541 aaatatcatc aaagtatgac attagcttaa acactttaaa gtcatggata aaaagataca
     3601 actgggctag tgaaaaaaag aagggtgcac ctataaataa aagaggtgca cccttttcta
     3661 ataaaaattc agttggtcat ggtgctccaa aagagaataa gaacgctgaa aagtttggtt
     3721 tcttctcaaa atatctaccc gaagaaacta gggaattgat acaagaaata tccataaaag
     3781 ataaatttga tattctttgg gagcagataa caatccaata cgcagcaata ataagagcac
     3841 aaaagataat gtatgttaaa gacaaggaag aaatgattaa agaattaaag aaacatgaaa
     3901 gtacagaaaa tggagagaag atagagtatg aatttcaatt tgcatgggat aggcaagcat
     3961 cttttcttaa tgcacagagt agagctatga gtgaacttag aagtttaatt aaacagtatg
     4021 atgaaatgat tcataaggat tggaatttgg ctacagagga gcagaaaaca agagttgaga
     4081 agttgaaatg tgaagttgat aacctaagta aagatgatat tggagatgat gagttgaaaa
     4141 taagtgtaga ttatggtgat agaaatgata gttagagtaa attttaatcc agatttcaag
     4201 gaagctaatt ttactaaaaa aagatacaga gcaatgaaag gttcagcagg gagtggaaaa
     4261 tctgttaatg tagcacaaga ctatatacta aagttaggag ataagaagta tcaaggagct
     4321 aatctattag tagttagaaa gtcagaagct acacataagt attcaacgta tgcagagctt
     4381 acaggagcta taaatcgtat ttatggtaaa caagctgata agtattggaa aactacttta
     4441 aatcctttag aaattaagag taaagttact ggtaactcta taattttcag aggagttaat
     4501 gatgcaaaac aaagagaaaa attaaaatca attaacttct cgaaaggaaa attaacatgg
     4561 gtttggtgtg aagaagctac agaacttatg gaaagtgaca tagacatact agatgaccgt
     4621 ttaagaggta ttttaactaa tcctaaccta tactatcaaa tgacatttac atttaatcca
     4681 gtctcagcta ctcattggat aaaaagaaag tattttgact ataaaaatga tgatatattt
     4741 actcatcata gtacttatct acaaaataga ttcatagatg aagcttacta cagaagaatg
     4801 caaatgagaa aagagcaaga cccagaaggg tacaaagtct atggtcttgg agaatgggga
     4861 gaaactggtg gagcaatact taaaaattat gttatacatg aatttactac agaatttgag
     4921 tattttgaca atatgaggtt atcacaagac tttggattta accatgcaaa tgtagtactt
     4981 agaattggct ttaaggatgg agagttatat atatgtaacg aaatatatgt acatgaaatg
     5041 gatacctcag aaatcataaa gattgcaaat agtataggtt tagaaaagac tctatttatg
     5101 tactgtgata gtgctgaacc agatagaatt aagatgtgga agaatgcagg atataaagct
     5161 aaaggagtta aaaaagggcc aggaagtgtt aaagctcaaa tagattattt gaaacaatta
     5221 agaatacatg tacatcctag ttgcactaat accataaaag aaatacaaca atggaaatgg
     5281 aaacaagacg aaagaactgg attatatctt gatgaaccag ttgagtttat ggatgatgca
     5341 atggctgctc ttagatattc tatagataat aagcttaaaa ataatggaat aagcttctta
     5401 aagtaaagga ggtgttaaat atttatataa gtgaaacaga tttaataaaa gttcagttaa
     5461 aaaaagagag cacctttaac ctagtaaaag tcatagaaca ctacatctta aaacataggc
     5521 cagaaaaata taaacaagga gaagaatact attatggtaa tactgatgta aacaataaga
     5581 gaagatatta tctcttagat ggagctaagg ttgatgattt tactaaagtt aataataaag
     5641 caattaacaa ctaccataag cttttagttg accaaaaggt aggctatagt gtcggaaatc
     5701 ccatagtatt taatgcagat gatgataatc tcactaagct tttaaatgac ttactaggag
     5761 aagagtttga cgatacaata acagaactat atctcaatgc tagtaataaa ggggttgaat
     5821 ggttacatcc atatattaat agaaaaggtg agtttaaata tgtaataatt ccagctgaag
     5881 aagcaattcc tatttgggat agtaaaagac agagggaatt agttgcattt attaggtttt
     5941 attatattga agatatagat ggaaataaaa taaaaagagt tgagtactac acagaaaatg
     6001 atgtaactta ctttgttgaa agaggtaata gttttgttca agaattttta tatgatgaat
     6061 atggaaaaat gactgatata caagaaggtc attttagaat aaataacaaa gaacagggat
     6121 ggggtaaagt tccatttata tcttttaaaa ataatgaaaa gtgtgtctca gatttaactt
     6181 tctataaatc attaatagat atatatgaca ataatatttc tacactagca gataacttag
     6241 atgaaataca agaggttatt tatgtattaa aagaatatcc aggaacaagt ctacaagagt
     6301 ttatagataa tataagatac tataaatcaa ttaaagtaga tggtggaggt ggagttgata
     6361 aactagagat aaatatacca gttgaagcta aaaaggagct tcttgataga ttagaaaaga
     6421 atataattat ctttggtcaa ggagttaatc cagaatctca aaacacaggt gacaaatcgg
     6481 gtgtagcact taaattttta tattcattac tggaccttaa atgttctaag actgaaaaga
     6541 agtttaaaaa agcaattaga gagcttttat ggtttgtgtg tgagtattta aagataagtg
     6601 gtagtaagag ctatgattat aaaacagttc aaattacttt taatcactct atgataataa
     6661 atgaagctga aaagatagat atggcagcta aatcaactgg aattgtatca gatgaaacta
     6721 ttgtttctaa ccatccttgg gtcgaggatg taaatgacga acttgagaga cttaaaaaac
     6781 aggaagatac tcaaaaagag tatgatgatt taattcctaa taatcaagat ggtgttatag
     6841 atgaaacata aagattattg gaggaagaga tttgaacaat tagaagaagc tcaaaataac
     6901 aaaagtgtaa aatattatct tgaattagaa aagcaatata aactagctat gaatagtata
     6961 gaaaaagata tattagcatg gtacaacaga tttgccaaaa atgaaggaat atctttatta
     7021 gaagctaaga aactactaaa tacaagagaa ctagaagagt ttaaatggag tgtcgaagaa
     7081 tatattagac atggtaaaga aaatgctata aatcaaaagt ggatgaaaga gttagaaaat
     7141 gctagtgcaa gagttcatat aacaagactt gaagctttaa agttacaaat acagcaacaa
     7201 gtggaagtgc tttatggaaa tgaacttgat ggtattgata aattaatgag acatatttat
     7261 acaagtggat attatcatgc agcttttaat gttcaacaag gagtaaacgt tggttggagt
     7321 ttaatgaatc ttgatactaa tagaataaat aaagttatct ctaaaccatg gactagtgat
     7381 ggattaaatt ttagtgaaag gatttggggc aaacatagac ctgctttaat taatgagtta
     7441 catactaagc taactcaatc aattattaga ggtgaaaatc caaagaacct agtaaatgac
     7501 ttttctaaga gatttaatgt atctaaatca caagctaaga atttgataat gactgaatca
     7561 gctttctttg catcagcttc aagaaaagat tgtttcaatg atttagatgt agagaagtat
     7621 gagattattg ccacactaga cttaaaaaca tcaaatatat gtagagagtt agatggaaaa
     7681 gtatttgata tgaaggatta tcaagttgga gttacagctc caccatttca ttgtcgttgt
     7741 aggacaacaa cagctccttg gtttgaggat gaagaaggtt acagagcagc aaaaggagaa
     7801 gatggaaaaa catattatgt accatctagt atgaagtata atgagtggta tgagaagtat
     7861 gttaaacata atagtatctt agaaataaaa aatagtgcta taatagatag cataaaagaa
     7921 gatattaaaa atggtaaata taatttaaat attcatgatg ggaaacaagg aaaacattta
     7981 aaagagcata ataattatat agaaggaaga agctatttaa ctataacaaa agaagaagct
     8041 caagaacttg tgaataagca tgctggcaat ggaatcataa aatttaatcg aagtggagaa
     8101 tgggataaaa aagaacttat agaagttgat aagaatatag gggtcaatgt taataatatt
     8161 acgggtgaaa aaactcttac aaataagttt aagatacatt attccaaaac tggaacacat
     8221 atagtaccag ctttataagg agggaaaata aaatgaaatt atgggaatat gtagggaaaa
     8281 atgttcaaat aacttgtgta gataagcaaa taataagagg aaagtgtgat ggatatacac
     8341 aagctttaga taatgaacca gaaatagcaa gtatatcaat agctagggat ggttatggaa
     8401 ttgaagttta tgaaaatgaa atagaatcta tcgaaaatat aaataaggaa tgactaagca
     8461 tgtacttaaa aataagtaga tgcttttatt ttgtaaaaaa tgaaaggaga aatttaaaat
     8521 ggattggtta aaagaattgc tagaaggaat aaaaatagag gataacaaga ttgatgtagc
     8581 ttctttacaa aagtctatag aaaagaaaat aaaagagact acaattactc aagaagatta
     8641 tacaaatctt gaaacacagc ttaatacagc taatgaaact attaaaaagt ttgaaggagg
     8701 tatgacaaaa gaagatgtag agaatctaaa aacaacttat gaaactgata agaaaacttt
     8761 ggaagaaacc tacaaaaaag aaattgaaga aaaggacttt aattactggt taaatgatgc
     8821 ttttaagtct attaaatgta gggatgaaat agcgttaaaa gctcatttag atatagaagc
     8881 actaagaaat agtaaagata gacaaaaagc ttttgaagag caaataaacc ctttgaaaca
     8941 ggataaagat tatttgttta atgcaacact agaaggtgaa gagcctaaaa tagatactat
     9001 aacaccaggg caagagccta agataaatga ttttggtttt aattttactg gggtaagacc
     9061 tcatgaaaat aataataaat aggaggaaat aaaatggcag cactaaatta tgcaaaagaa
     9121 tattcaaatg ttttagcaca agcatatcct tatactttaa acttcgggga tttgtatgca
     9181 acaccaaata atggaagata tagatggact ggttctaaaa caatagaaat accaactata
     9241 tctacaactg gaagagtaga ttcaaacaga gatacaatag cagtagctca aagaaactat
     9301 gataatgctt gggaacctaa ggtattaact aatcaaagga aatggtcaac attggttcat
     9361 ccagcagata taaaccaaac taattatgtg gcttcaatag gcaatataac aaaagtatat
     9421 aatgaggaac aaaagtttcc agagatggat gcttactgta tatctaaaat atatgctgat
     9481 tggaccgcat taggtaatac agcagataca actgttctta caacaacaaa cgtattagaa
     9541 gtatttgata agttaatgga aaaaatgaca gaagctagag tacctgaaaa tggaagaata
     9601 ttgtatgtta ctccagtagt aaatacactt atcaaaaatg caaaagagat acaaagaaca
     9661 gtaaatataa aggatgcagg aacttctctt aatcgtcaaa caactgatat tgacacagtt
     9721 aaaataatta aagtaccatc taatctaatg aaaactgcat atgattttac aactggatgg
     9781 aaagtaggag caggagctaa acaaatcttt atgtccttag ttcacccaag tgcaataatt
     9841 acacctgttt cttatcagtt ctctaagtta gacgaaccaa cagcagttac agagggaaaa
     9901 ttcttctact ttgaagaaag ttttgaggat gtatttatat taaataaaaa agctgatgca
     9961 atacaatttg ttgttgaagg agctggagca taatggcaca agtaaggaaa ttaaatagaa
    10021 tattaaccat agaagagtgt aaaatagatg atttcttaga gatgggatat gatttgatag
    10081 atgaaactgg taaggtagta aggtatggca agtcattaaa tgtaaaagat ttaatagctg
    10141 aaaataatat tttaaggtca aaagttgagt ctttagaaga agaaaataag cagcttaaag
    10201 agaaaaataa acttactaaa aagtaggtga aaattatggg aaataatata attgatgata
    10261 tagaaaaaag acttgaaagt tttggatata tattaaaaga tggggataag tggttaatag
    10321 attttgtaag agaaaaaata gaaaatatta ttaaactaga ttgtaatata aaaactatgc
    10381 caattgaatt gaaagaaatt gaagctgata tgatagttgg agagttctta tttaccaaga
    10441 aaaatatggg gcaattagat atagaaagca ttaactttga agctgtagaa aagtctatat
    10501 cagaaggtga tacaaaggta gatttcgcta taggaagtgg ctctcaaaca ccagaacaac
    10561 gctttgatag cttaatagct tatcttactg cttatggtaa gaataagata ttaaccttta
    10621 ggtgcttaag atggtaagta aaactagaaa agcaatagaa atgttatata gatataaatg
    10681 tactatagtt gagtatcagc caatcaaaga ccctgtaaca aaacgaacta acaataaaga
    10741 agtgattgta ttagaaaatc aaccatgcaa gctttcatat aaaaatatag tttctgctac
    10801 agaaggaaaa ttagctaagc tagagcaaac tattaaactc tttatatctc cagatataga
    10861 aattaaagca ggttcaaaac ttattataaa tgataaagag tatgtaagaa gtggagaatc
    10921 agctatatat ccaaatcatc aagaaataat acttgagtta tttaaggata aagcataatg
    10981 gctagatggg gcagtgttga ttttagagag tttaaaagag tttgtaaaaa gatggagaag
    11041 cttacaaaga ttgatttaga taagttttgc aaagatgcag caagagaatt agcagcaaga
    11101 ctcttaggaa aagtaattag aaggacacca gttgatacag gattcttaag acaaggatgg
    11161 aatggagtgg cttatgctag gtcgcttcct gtgtataaac aaggaaataa ttatattata
    11221 gaggttgtta atccgactga atatgcaagt tatgtaaatt tcgggcatag aactaaagac
    11281 ggaaaaggtt gggttaaagg acaacatttc ttaacaattt cagagatgga actacaaagc
    11341 caagttgata agattataga gaaaaagtta ttaatattac ttaaaggagt atttgatgct
    11401 taataatatt atagatggaa tatcagtaaa gttagataaa tcatttggag aaaaatatac
    11461 aatttatagt gaggatgtag agcaaggtat taatgaacct tgttttttta ttgttccttt
    11521 aaatccaagc aagacaccat atccaagcgg gagagaatta aagaaaaatt cttttgatgt
    11581 acattatttc cctcgttcag aagctaagaa ttttgaaata aatgagatag ctgagatgtt
    11641 actggaagaa ttagagtata tagaaatcaa tggagattta gttagaggca caaatatgaa
    11701 ttttgaaatt atagacaatg ttcttcactt ctttgttgat tataactact tcactataaa
    11761 gagtaataat gcagataaaa tgaatacagt agagttattc ggtggtttga agagaggtga
    11821 taattttgag taagacatta agcaaaggaa ccgattacaa gtttactaag gagcagatag
    11881 ttaattctaa gaagtatgta aatagaaaag acttattaaa tgcaatttta aaagaaaatg
    11941 agttatattc cttctcagaa gtagaggata gaataaataa attcatgaaa ggagtgagtt
    12001 agatggcttt aggtggagga acatttgtaa cacagaataa agtattacca ggtagctata
    12061 taaactttgt aagtgccaca agggcaacca gttcacttag cgatagaggt attgttgcaa
    12121 tgcctttaga acttgattgg ggaattgatg aagaagtatt tcaagtgact agtgatgatt
    12181 ttgagaagta ttcaactaag tattttggat atgattatac acatgagaag ttgaaaggtt
    12241 tgagagattt atttaaaaat ataaggctag gatatttcta taaattaaat aaaggtgtta
    12301 aagctagttg tagtattgct acagctagat gcagtggtat aagaggtaat gacttaaaag
    12361 ttatagtaac aacaaatata gatgataatg ctaaatttga tgttgtaaca cttttagata
    12421 ataagaaagt tgatactcaa atagctaaag ttattacaga actgcaagat aatgattatg
    12481 tcacttggaa gaaggaagca acattagagg ctacagcagg attaactttt actaatggaa
    12541 ctaatggtga agctgtgaca ggaacagagt atcaagcttt tcttgataag atagaaagtt
    12601 actcatttaa tgcactagga tgtttggcta caacagcaga gattaaaagt ttgtttgtag
    12661 agtttactaa gagaatgaga gacaaagttg gggctaagtt tcaaactgta ttatataaaa
    12721 agaatgatgc agattatgaa ggtgtagtat ctgttgaaaa taagattaaa gatactggat
    12781 tattagaatc tagcttaatt tactggacta ctggagctat agcaggatgc gatataaata
    12841 aatctaatac taacaagcga tatgatggtg aatttgatgt agatgttaat tacactcaaa
    12901 tacatttaga agaagcttta aaaactggta agtttatatt tcataaggtt ggagatgaag
    12961 ttcatgtatt agaggatata aatacttttg ttagttttac agatgaaaag aatgacgact
    13021 tttcaagtaa tcaaagtgtt agagtacttg accagattgc taatgatata gcgactttat
    13081 ttaatacaaa gtacttgggt gaagtaccaa atgataaatc tggtcgtatc tcgttttgga
    13141 atgatgtagt taagcatcat gaacaactgc aaaatatgag agcaatagaa gatttcaaag
    13201 ctgatgatgt ttctgtagaa cctggaagcg acaagaagac tgttgtagta agtgatgctg
    13261 taaaagttat tagtgctatg agtaagcttt atatgactgt ttcagttagt taacaataag
    13321 aaaggagaat aataatggca caacaaataa aagcaagaga tacaataagt gcatctaagg
    13381 cagagtgttt tgtaactata aaaggtaaaa gatataattt tatgcaagct attaacttag
    13441 aagctaaaat ggaaaagaat aagagtgaga tacctatatt aggtagtact acaaaaggaa
    13501 ataaatcaac aggaagtaaa tattcaggaa atgcaacatt ttattataat acctctatat
    13561 ttagagaatt gttgtatgag tataaagaaa ctggtgagga tatttacttc gatatacaaa
    13621 ttaccaatga agacccaaca agttcagtgg gtcgtcaaac tataatactg gaagattgca
    13681 atatggactc aggcataatt gctaaatttg atgctgatgg ggagtattta gatgaagata
    13741 tggattttac ttttgagaat tggaaattag ttgagaaatt taatatagca aatggtatgg
    13801 agtaaaatac acatttatga tttatgtatg tgtatttttt atttataaga ataggagatg
    13861 attaaaatta aggataaata tgagataaaa gattcaattt cttttgatta tagcaataaa
    13921 agacctttgg aagaacgtgt cagcgagatg tataaaaagg caggaaaata tcttatagat
    13981 atttcagata agttagcaac agatacaatt gatggttcgt cattaaagcc aataatcata
    14041 aaatttgaaa taaatgaagc tggtgttgca acaatagaaa aacaaacaaa atatttggtg
    14101 atggaggtag aataagaata tgggagattt aaacgctttt ttaagtcaaa atgcaataaa
    14161 agtagagaat agaaagtatg tggcaagtga aaggtttata ggagaagatg gaaaagcaat
    14221 cgaatgggaa cttaaagcaa tagattcaga tagagataga caattaagaa aagattcaac
    14281 tataagagta cctgtactaa ataaaaaagg gaaagcaaca gggcaataca ctagtgaaac
    14341 agattttaat acttatactt tgaaactgtg tgtagaaact gtagtatttc cagatttaca
    14401 tgatgcagaa cttcaaaata gctatggtgt aatgggagca gaggaactat taacaacaat
    14461 gttaactcct ggtgaataca cagacctttc aagtgaggta ggagaagtga atggttttga
    14521 taggactttt gaagataaag tagaagaagc aaaaaactaa ttgaaggagg cgattatgat
    14581 gctagtgtag ctcattattg ccttcataaa ttcaaatgga aaccacatga atatacagat
    14641 ttaccagact tcgagagggc atttgttgct gcttctatag atattaaagt agaagaagaa
    14701 ataaaagaag aaaaaaagat tgctaaagaa gctagaagaa gtagaagaag ataaaatata
    14761 ggtaaaatat gtaagaatta tatgttataa taattgtagc aagaagatgt aatctacaat
    14821 ttatagagtg gagttcatac tgggataaaa cctacttcct aatgaaagga ggtgggaagt
    14881 atggacaatt ttttacttag tatattagct agcttgatag ctagtttaat tggatatatc
    14941 gtttgtagat gtatcaaaaa cgtaaaaagc cactctactc gtggcaagag taaaagtggc
    15001 tgggaacttg gttttaaaat aaagttccgc aaatttaaat aatttatatt ttttaaaatt
    15061 atgaacttca ctctaccgct aaatagattg tagttcttct tgcctttatt ataccacaaa
    15121 ttagaaaaaa tatgcaaagt acttgtttta atagtaagta ctttttttat gtgaaaaaga
    15181 aggtgattga atgaataaag atatagaact tacagaaaaa gatttatatt gtattgctag
    15241 acatattcaa gtgtatgtgt ttaaaaaaga tgatgaggtc ataaaaaagg aagataatcc
    15301 atgttgcaga tgcaagcata aatttaagta ttatgagaat agtgtatgta tctatcattg
    15361 ttcagtattt cagaagttat ctaaaattac aggtcttaaa atgggttttg gagtcaaact
    15421 ataatgtaaa aattttgaat atatagtata atattcttat aaaatataat ttagggggat
    15481 attatgagag aaggaaagag aagaagagga tgtctatttt ggtttatcat tattattata
    15541 ttttctgggg ttgttggagc aatagcaggg aacagtacta ataatggaag cactgaaaaa
    15601 caaaaagaag atttaactaa atatattggt gaagaaggta atataggaga tttaaaatta
    15661 actgttaata gtatttcaaa agctagtgaa ataccagtag cttctggtta tttggcgtac
    15721 actccagata gtggcaaata tggtattata aatttaacaa taaaaaatca aactaaggaa
    15781 agccaatctt ttatgctaaa ttcattcaca ttaataggac cagataattc aaaatatgtt
    15841 ccatctttat tgattgatgt aggaaataaa tatataacta tggatactgt aaatcctaat
    15901 ttagatgtaa caggtaatat tgcatttgaa attcctaagg atttactagt ttctgattgt
    15961 aaactaaaat atagtggttc agagcaagaa tttatattaa aagaaaaata atatatagat
    16021 ttaaaatcaa aaatactaaa aacacttact aatgtaggtg tttttttaat gaaaggatgt
    16081 gatgataatg taaaaatttt actaatatag tataatattc ttataaaatt attatactgg
    16141 gggggaaatg ttatgttttg ttcaaattgt ggtgcagaaa tcacaggcat aggcaagttt
    16201 tgttcaagtt gtggggttgc tgtagaaact gaaattattg aagataataa tattaaatca
    16261 aatgacttaa tcgttgatgc taatggaata gaaataaata tgactgaaat ttatagaaaa
    16321 tatagaaaag aaaaagtaaa cgcaataaaa aatgtaatgg aaataagtgg tttgaatata
    16381 aaggaagcaa aaaaaatagt ggatttttct tttgaagaat taaaaattaa ttttattgat
    16441 gatactatga gtaattcaga aaaggaaaaa ataatacaaa atcaaaatag aaaaaataat
    16501 attgaaaaag ctcaacaaga atcagttgct tgttgcccta agtgtggttc aacatccttg
    16561 acagctcaaa aaaaaggttt tggtataggg aaagcaatgg taggagctag tctaactggt
    16621 ggtataggtt tagtagctgg aaatttagga gcaaagaaag ttagagttac atgcttgaat
    16681 tgtgggaagc agttttgggc aggcaaaaaa tagatgtatt aattataaaa acacttacta
    16741 atttagtaag tgtttttatt atctaaatta acagaaagga gagtgaaaaa atggctacaa
    16801 tacagacatc aattcgcata tttgatggaa tgacacctgc gtttcggcat atgacaactg
    16861 ctatgaacat agtactgagt tcatttgagc aattacaaag aacttctagt aatgcagtaa
    16921 atgctaatag cataataaga gctagagaag aactagcaag agcagaagct ggatttgata
    16981 gattagaacg acaaataaga gaatcagata atcaacagcg aaaacttaat gaggatataa
    17041 ataagggtgc aagttctaca gatagattag ttggaagtgc aaagaagcta gcagcaactt
    17101 atttaggtat aagaacatta ggaggtctag gaaatttaag cgaccagatg acaagtacta
    17161 atgcgagact tggcatgata aatgatgggc aacaatcaga tgctggactt aataaaatga
    17221 tatttcaatc agctgaaagg tcaagagcat cttacttaga tactgcacaa atagtgagtc
    17281 gtataggcat gaacgcagga aaggcgttta gcagtacaaa agaaattgta ggttttgcag
    17341 agcaattaaa caaaaaattt gtaatagcag gcgcaagtac tgaggaaatg aactcggcat
    17401 tgttacagct aacccaaggg ttgagttctg gcgtattaag aggtgaggaa ctgaatgctg
    17461 tgtttgagtc agcacctaac atcatccaat cgattgcaga ttatttggac gtggacatag
    17521 gaaaaataag aggaatggca tcagagggaa tgttaacagc agatattgta aaaaactcat
    17581 tacttgcagc agcagagcag accaatgcag agtttgaaaa aatgccttac acattttctc
    17641 aaatttggac ttcaattaaa aataatgcaa tcatgatatt tggtgttata cagaaaaaaa
    17701 ttgaacagtc tatgtctagt aagggatttc gaacctttat agataatttt ataaactctt
    17761 tgtatgtact tggaaatgtt gcttataaca tttttaatga aattataagt atattaggga
    17821 gcccgttttt tcaagcattt gtaaatgcga ttattgtagg tgttagttta atagtgcagg
    17881 cactaggttg gataataaca caggcattaa atattgctaa tgtgtttgct cagaactgga
    17941 gtattattgc accaatagta cttggagttg cggctgctat gttagtgtat aacaatgcgt
    18001 tattacttag tattgcgaat aaagttaaag atattgcact atctgccaaa tctttagtca
    18061 tgagctttgc acatatagta gcagagtctt atagagcagc agctttagta gcaactacaa
    18121 tagcacaaga cggattaaat gcagcaatgg cagcttgtcc tattacttgg attttatatg
    18181 gaattatagc tatagttgtt gcattttttg tagctatagc aatatttaat cacttcgcag
    18241 gcactagtgt atctgctata ggtgtagttg caggtgcaat atcagttgca gcctctttca
    18301 taggaaactt atttattgca acaggaaatt taattataga tattgtagct ttaatataca
    18361 atactttagc aggctttgca gagttctttg ctaatttttt agacgaccca attggctctg
    18421 ttataagagc agtatccgga atggctaatg ctgtattagg cattataaga agtatcgcaa
    18481 gtgcatttga taccgtgttt ggttcaaact tggcagatgc agtaagtgga tggcaagata
    18541 aattgcaagg ttggactgat aaagtagcag gagaagctaa aataaaagtc gaaagaatgg
    18601 accctaataa actgcatttt gacagattta attatggaaa agcatgggac gcaggatata
    18661 aatggggaga taagttagaa actaatataa aagataaatt tgatattagc aaaatggcag
    18721 aagatgcaaa gaaaaaatta ggattggatg atttatggga taaaaaatat ggattaggag
    18781 atggatttgg ttcagcagga ttaaactctc ctctcaatga tgcagcaaaa ggagcaaaag
    18841 acactgcggg aaatacagca aagatggcta aaacaatgga taaaagccaa gaagacttaa
    18901 aatacttaag agacatagca gaacaggaaa caatcaatag atttacagga gtaaacataa
    18961 aaattgatat gaacaacaca aacaacataa gtaaagataa tgacttagat ggaatagtta
    19021 atgttctaac tgaaaagtta aatgatgcaa tggctgtttc agccgaagga atagtttagg
    19081 aaggagagtg agaaaatggc ttatgatttt tacctagatg gagtacaact accaatcgca
    19141 ccaccaaagc ttgaagtcaa agtgacaaat aaaaacaaga cagttgattt aataaatgtt
    19201 ggagaagtaa atatattaaa aaaagaagga ttatctgaaa taagttttga agcagaattt
    19261 acacataata aactaccatt ttatcgtgga acttttaggg atgttcaatt ctttttaagt
    19321 aaactggaac tactaaaaac tgattgtaag ccatttcaat ttattgtatc gagggaatta
    19381 ggtaataaag tactatttaa cactaatata aaagtatctc ttgaagagta tgctatttca
    19441 gaagatgcag ataatggctc agatacaaaa gttgcaataa aattaaaaca atatagagat
    19501 tactcaacta aaaagttagt tcctgcaact cctgaaaaga caaactatgg taggactccc
    19561 cctccagtca tgaaaccaaa agaatttaga ccagattcat ccaataagcc aaatggtaaa
    19621 acatatacag taaaagcagg ggattctctt tgggcaattt gtcaaaagca attaggaaat
    19681 ggttcgttat acaagaaagt atatgagtta aataaaacaa tgatggataa agctaacaag
    19741 ggaaaaaaat taagtaaata caccatctat aaagggcagg tgttgaggct tgtctgatga
    19801 tttagttctg gcgaatgata gagatataag gctagttatt gcacattggg aagatttcta
    19861 cgaacctgta gttttggatg gtatcacatg ggaaatagag agacgaggaa caccatctaa
    19921 acttgaattt acaatagtca tggatgatat attagagttt tgtgaaggta actctgtaag
    19981 actatattac aaaggtgtag gtatattcta tggatatata tttcagaaaa agagagataa
    20041 agaaaatcat atcaagatag ttgcttatga ccagctaaga tattttaaga ataaagatac
    20101 ttatgtatat agtaataaaa cagcaagtga acttgtaaag atgttggcta aggattttaa
    20161 attaaaatac aatgtcatag aagatacaaa atataaaata tcgagagttg aagaaaataa
    20221 aacactcttt gatatggtct taactgcact agatgatact ctaagagaga aaaaggaaat
    20281 gtatgtttta tatgatgatt ttggaagaat aacattaaag aatgttgctt ctatgaaact
    20341 tgatacggtt atgaacaatg atgtaattga ggactttgac tacaattctt ctattgatag
    20401 tgatacttac acaaagatta aacttgtgag agataatgaa gaaacaggaa aaagagatgt
    20461 gtatattgct caagactcaa cgcatatgag gagttgggga atactacaac tatttgatac
    20521 agttgataaa aatatgagtg aagcagagat aaaacaaaaa tgtgatatac tcctaaaact
    20581 atataataag aaaactaagt cattaagttt aaaaaatgtg ttaggagata ttagagtaag
    20641 agcaggttgt ttagtacctg tttttttgtc gttaggagat attgatttac aaaattatat
    20701 gttagttgag aaagtaaaac atacatttga aaataacagt cattttatgg acctaacttt
    20761 ggttgatgga gacgaatttg cttcttattc ttcatcaagt tatagtagtg gaaatactaa
    20821 taataaaaat gagaaacaaa atggtcctgc acaaagtact acaagtaaag aagatactga
    20881 tatggctaat aagattaata aactacttaa aggtaaatta tcaaatacag gaaatatatt
    20941 tgttaaatat tcaaatgctt ataaagttaa tccagcactc atggctgcta tatctatgca
    21001 cgaatcagct agagggactt caaatattgc aaatactaaa aataatttct ttggaatgaa
    21061 aaaaaatgga gattacatga gtttttctag tgtagacgaa ggaataaaaa gaggtataag
    21121 taatttatca agaaactata tccatatagg acgaaaaact ttagaaagca tcagaaataa
    21181 atattcttct agttcagaca aagaatgggt aaaatgtgta ggtgcatttt ataagcaaat
    21241 aacaggaagt acttataatt ctaatagtgc aggcacagga gttggaagta atgaagaagc
    21301 agaaaagaat ttaaaagatt taacttatca agttcaaaac aataattcta atacatcaac
    21361 aaacaataat aataaagtaa gtaaagttat tcaagaagca aaaaatcaac ttggcaagcc
    21421 ttacaaatgg ggtggtaatg gtccaaagag ttttgactgt agtggtctta tggtgtgggc
    21481 atttaaaaga ggtgcaggaa taaatctcaa aagagtttca gcagaccaat caaaagatag
    21541 tagaggaaaa ctattatgta acataaatga tgtaaaagct ggtgatttag tattctttgc
    21601 atacaacaaa ggaaaaggaa atgtacatca tgttggacta tatataggaa atgaccaata
    21661 tattcatgct ccacaaactg gtgacgtagt aaaaataagt agtttaagtg gtagacaaaa
    21721 gaaaaagcat gattttgcaa gagctagaag attcttttaa gtgaggtgat aaagtgtcac
    21781 aagaattatt gcaaataatt aagaaggctg caatggatgc agtagaaaca agcaatccaa
    21841 tgcaaattgc atttggaact atagaaagtg ttaatccttt gatagttaag atagaacaaa
    21901 aagcatcttt tgaagaattt tttctaatac aaacagagac ttttaaaaga tatacagata
    21961 aaaaaatagg ggataaatta gtcttaatta ggatgcaagg aggacagcaa tatttgattt
    22021 tagataggat gtgatgaaat gttaccaaca gataacattg actatgatat agaagatgta
    22081 tcgataatta attttgatgt tagacaagaa ccaagtaaga cgtttaaatt gaatatagag
    22141 aaaaatagag tagatggtat ttgtgatgat gtagaagcat taaaacaaac cattttttta
    22201 attttaaata ctgaaaggta tgagcacctt atttattcta gaaattatgg tgttgaatta
    22261 aatgatttaa ttggagaacc tatttcatat gtaatacccg aacttgaaag aaggataaca
    22321 gaagcactaa ttcaagatga taggattgaa aatatagata attttgagtt tcaaaatata
    22381 aagggtaaag tacaatgtag attttcagtt catacaaaat atggaaatat aaaagcagag
    22441 aaggtggtga gtgtataatt gtttgagtta atgacatttg aaaatataat taaaagaatg
    22501 ttagatagtg taccagatac ttttgataaa agggaaggtt ctataatata taatgctctt
    22561 gctcctgttg ctatagaact tacagaaaca tacattgcta tggatgaatt actagaccaa
    22621 acattcgtag atactgctag ttattattat ttagagaaga gatgtaaaga gagaggtatt
    22681 acaccacttg aagccactaa tacaattgca aaaggagttt ttaacataga tattcctatt
    22741 gattctaggt ttaatctagg agaatataac tatgtagcaa ttgagagaat atctgaaggt
    22801 atatataaga tgaaatgtga gactgcggga cctatttttg agttgggaca actaatacct
    22861 atcgaatata tagacaaatt agaaacagca gaactgacag aaatactgat aaatggagaa
    22921 gatgaagaga gtgaggatag tttaagacaa agatattatg atagcctaaa ttcacagagc
    22981 tttggtggaa atatgcaaaa ttataaagat gaagttaaca aaatacaaga tgttggagga
    23041 gttaaggttt atcctgtgtg ggacggtgga ggaactgtta agttagtaat aattaactct
    23101 aatttcaaag taccatcaga ggatttagtt aatttagtgc aagaagaaat tgacccaatt
    23161 ggacatcaag gacaaggctt aggattagca ccaatagggc ataaagttac tgttacaggt
    23221 gttgtaagta caactataaa tatatcagca gagataacat acaaaaatgg ctacacttgg
    23281 gagaatataa aatcaattgc agaagaagca atagacgact atttaaatga acttaacatg
    23341 agttgggaag atgaagaaaa cttaatagtc cgtatatctc aaattgaaac tagattactt
    23401 agtattgatg gagtattaga cattgcaaat acaatgataa atgaggttaa atctaatcta
    23461 acaataaata gtaacagtat agtagtgaga ggtgaggtag ttggataaag agattaatct
    23521 aataaattac ttaccacaaa ttctacaaga taaagaagaa tatataaaag tatttaatgt
    23581 aggaaataaa gaaataaaaa tattacatga taaattaaag gacctatcaa atgaccagtt
    23641 tttagaggac ctaactataa gtggtataaa aagatgggaa aagataatgt ctataactcc
    23701 taaaagtaat gagagtttag aagatagaag gtttaggatt tttagtaaat atataagtaa
    23761 attaccttac tcagagagat ttttaaggaa ttggctagat agtatagttg gagaaggtaa
    23821 ttatgagtta actattaata atgctactta taatatacat cttgaaagtg atgctagaaa
    23881 tcaagattgg tttgaggaag ttcattcttt tgtaagtaat attaagccat gtaatatgac
    23941 tttagattac actagagtgc ttataagcaa agacaattat atgaattttg gtataacaac
    24001 cctaatgggt caagaaataa ctatataccc ttggagtcca ccagatatag aaacttatgg
    24061 agaaattgat gtattaactg gcaatggagt tggataccaa gagataacaa tattttagga
    24121 ggtgatatat tggctataga taaaagttat tacactataa ttacagatgt aggaaaagca
    24181 aagatagcaa atgcaagtgt cacaggtaat aaagtgggat ttgtaaaaat tcaacttggt
    24241 gatggaggag ggagtgaata tactccaact gagagtcaga cagctctcaa aaacgtggta
    24301 tgggaaggca atattggaaa tacaactaca gatgaaactg caccaaattg tataatatta
    24361 gagagtttaa taccatcaag tgtaggcgga tttatgataa gagaaatagg atatttagat
    24421 gatgaaaata atttaattgc catttctaaa tacaaagagt gttataaacc ttctatagaa
    24481 caaggtgcag tggtagacat gaaggttaaa actgtgctta ttgtatctaa tgtaaataat
    24541 atagaactta aaattgaccc aacaataatc tttgcaacac tcaaagatat acaagactta
    24601 gaaactaaaa taggtactgt taatactaaa atagatacaa ctaaaacaga attaacaaat
    24661 aatttagaaa ctgctaaaac agagttaaat actagaattg acacagaaaa tgagaaacaa
    24721 aatattaaaa ttgaccaact tattgcaggt ggctctaatg tggcatctac tcaaacaata
    24781 acaattga
//
LOCUS       DAMJVI010000048        24664 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.050, whole genome shotgun sequence.
ACCESSION   DAMJVI010000048 DAMJVI010000000
VERSION     DAMJVI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24664
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.050"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..378
                     /locus_tag="PC545_002068"
     CDS             <1..378
                     /locus_tag="PC545_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HDF2456835.1"
                     /translation="AIFMLDKKQEVLETKIENVNEKLENFMDDAPLFNIECECIVKEV
                     KRVATKSLGGHGSKAYKNKSLRGKVYSDIYHQIKREFGVDSYKAIKRCQLDKVLEIVN
                     SYKLPIVFEEEIRLLNSQLSIVS"
     gene            521..1009
                     /locus_tag="PC545_002069"
     CDS             521..1009
                     /locus_tag="PC545_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456836.1"
                     /translation="MDNNINKKELFKKVEGRLHHYKFLSAEIKNLELDIESRENEIFG
                     CKAIGYGEKVSPTYAFNSTVENEIIKKERDIARLKKLKKDKEIEKKKIENALTCLDIR
                     EEHFFKLFYNSRMKNSMVYISLEMNSDRKTCRCVRERLVYKVMDMLYPRIKENELPLF
                     KN"
     gene            1591..2268
                     /locus_tag="PC545_002070"
     CDS             1591..2268
                     /locus_tag="PC545_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDF2456837.1"
                     /translation="MLKILQEKNVKVMWSKNGEEVWFNANDVGEELGIVNIRDTLRNI
                     DREYKKKFNESTVGDSYTRNFKDKLPNFGTTFVTEEAVYNMSFRSNKPEAKLFTKWVT
                     KTLKQIRIHGYYIATEKDQEWLDIRTEGKKVRKDFTDEIQEFVYYATSQGSNKPQMYY
                     KHFTELVRKKLGIPKGVKRDELNQSELFDIQALERIISMKLPKLIDKDMNYKEVYKKI
                     KELIEMI"
     gene            2331..3032
                     /locus_tag="PC545_002071"
     CDS             2331..3032
                     /locus_tag="PC545_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456838.1"
                     /translation="MQDVKEKVKQDYIKGMKQKEISAKYDISLNTLKSWIKRYNWASE
                     KKKGAPKNKRGAPIGNKNATGPPGNKNAEKFGFFSKYLPEETRELIQEISIKDKFDIL
                     WEQITIQYAAIIRAQKIMYVKDKEEMIKELKKHESTENGEKIEYEFQFAWDRQASFLN
                     AQSRAMSELRSLIKQYDEMIHKDWNLATEEQKNRVEKLKCEVDNLKKSDTGDDSKIWV
                     EAIQNIAMKRGVNNG"
     gene            3025..4434
                     /locus_tag="PC545_002072"
     CDS             3025..4434
                     /locus_tag="PC545_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDF2456839.1"
                     /translation="MDKALLTLLDCYWDNPVWFAEDMLNFKADKWQSDVLMALAQTPK
                     VSIRSGQGVGKTGLESIATVWYLSTRPFPKVVATAPTRQQLYDVLWAEIAKWLSNSKV
                     EKLLEWTKTKVYMKGFEERWWATARTAVKPENMQGFHEDYMLFVVDEASGVADPIMEA
                     ILGTLSGAENKLLLCGNPTRTSGTFYDSHNRDRDLYKTFKVSSLDSPRTSKDNIEMLK
                     RKYHEGSDPWRVRVLGEFPKGESDSLISLEAVETSTIREVNISNDYILNIGADIARYG
                     DDETIIAPRIGGKVFDLLTYSKKDTMETVGNILRAVDKFKSMYHQINRVKIKTDDDGL
                     GAGVTDRLKEVIRQERLKYEIVPIQNGSSAIEKDKYYNKASEMWDNMREELDANLSGF
                     IQNKEAIIQLPNDDKLIKQLSNRKYTVDSKGKIQIESKKEMKKRIGESPDRADAVIYS
                     FAENNNTDLSLLKGGSVWG"
     gene            4425..5927
                     /locus_tag="PC545_002073"
     CDS             4425..5927
                     /locus_tag="PC545_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDF2456840.1"
                     /translation="MGIISYVKKLFKRPTGEIMRMSSGNIGVYKLDDSRVDYELAREL
                     YQNKNANYKLGSSFVRPIVNSTTGFMGVPHFQIEDEEAQYILDEFVLDNTSKMLKTHT
                     DSLKQGDCYIWITREERENPLYPDKKVRLIYNFISPEEVKEIILDPTTKEPIAYILES
                     QNEWTDLEENKRRAKVKQIITAESRIIEVEGDKIEGLEEGETPNVWGFIPIIHFKNEA
                     DETLKYGQSDIEPIEPLLKAYHDVMLHALKGSKMHSTPKLKLKLTDVASFLAHNFGVE
                     DPVKFAKEGGKINLDGHEILFLNKDEEAEFVEVKSAIGDAKELLKLLFYCIVDVSETP
                     EFIFGVHTPSALASVKEQMPIMVNKIRRKREQFTNSWQLLARMVLIMSSNSSGMKYSS
                     YDVTIGWDEVNPRDDKELAETLEKVCSALDKALEGGFISEESTVNFLAQYIDTMSNYI
                     SDDPEIVGEREKIIKTKMLKYRLDDSQGLDDESNEIDKEIDKIKDNNDNG"
     gene            5920..6927
                     /locus_tag="PC545_002074"
     CDS             5920..6927
                     /locus_tag="PC545_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456841.1"
                     /translation="MDKSTLELITVAGEYKKWALEARKKFIDLRLKQDDEIRTMYINI
                     TRNITKEIRKGNLSDFNKVRLKQILKQLTQEIKILNEQLVFNFDEYLNKNVETATSYS
                     KNILINAVETAQITKVTKAMIQKAFYDINIRTVEAYYTRVKDGLFLSDRIWSKCKKYR
                     EDMKVILQTAVTEGQDCVKTAKMLDKYVLKGKKTLVDEYPNMIKRIGNRVPQNISYEA
                     LRLARTEMTSAYGDGVLASAMINPATIGIQFMLSMAHPHTDICDEICGEDNFGLGKGV
                     YPINEAPAYPFHPHCLCIMLTVVQPLDILVGRLKNWIKNPMNDTPLEMWYQEVYGNLN
                     F"
     gene            6947..8281
                     /locus_tag="PC545_002075"
     CDS             6947..8281
                     /locus_tag="PC545_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456842.1"
                     /translation="MNVITGEMDSMNALISSIKPSDIPLAKDIDIEALKSIDDDPLEV
                     VVEIPATKSKRGWNYTAKSLKDIVDYTNENTLNGFLGHQKAENISTEFAPPVTHWIGA
                     EMKGDKAYFRGLIDADATNLKRWIRTKRIKEVSIFGYPKLKKSAKGEMNVIGYEPLSI
                     DWTPLHRPGMPTSIVGMEMSPNGEQLDGTFEALRIDLREALKAKFSINDNNSYLYIQN
                     IRYDNNTVIYELEQNGLCKLYSIPFTIVENKINLGEEIEVIKKISYEAKGEMKGEENK
                     LEGKELIKNVKGLLQTGEISYSEVIQGIGLTKEIVTGEMEDVKSSLKAEKELREVKKV
                     LGIVGEMDTVEVAKKASKALENEKKEAWNCIVNKVIKDKVSGEIAQTLVKKMLNVEEG
                     SSEEVITGEIENILNDEFVKNTMSNMYKDNPTTTGLLNSSNNGSLTTKKNRI"
     gene            8297..8680
                     /locus_tag="PC545_002076"
     CDS             8297..8680
                     /locus_tag="PC545_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDF2456843.1"
                     /translation="MAFKGQPTPSTITQITRAKISDGKSVRVILSEGESTKTQQFYLI
                     NGFFGVAMQDGEKGDEVTLQIEQAEYETDNIVTSEAFEAGKLIYWDNTAKKFTTTSAS
                     NRLVGRVTDGKDSNNVIWFILLPQQ"
     gene            8699..9736
                     /locus_tag="PC545_002077"
     CDS             8699..9736
                     /locus_tag="PC545_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDF2456844.1"
                     /translation="MAFKVISQENLLEQKRKETLQEDIPFIVNGEMEYVTKKISNGEM
                     ETLELNKPLGEMMTFSSTSNLKELLRKVVLDVELGREQVQLLYKPIYDSIADSNLPQV
                     MDAKWALQGNCVFLEHIEGEEIKFGTINAENGPVARIQTYATGFEYTKEMKDFNQTFS
                     VEILNKSIGESYNALLNHIHLSPIINFNYKASNKTAFKGETNDPIWLGIWRTLTQAQK
                     DTVIAKRQGNILMASSADQIEIEMALNGGHLLNGSMYPSIKNISTVIYYDGWEVTVGK
                     KTYSYKGVTPGKGYLIRPKRGFKELIKRDLTTEVGNADLSKLVENQIVGHCYRGAFAA
                     VEENVQEISFR"
     gene            9776..10114
                     /locus_tag="PC545_002078"
     CDS             9776..10114
                     /locus_tag="PC545_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456845.1"
                     /translation="MTPTSNLIEKLRLLLNDKDKKSFTDEELNLFLEEADCIYCAASQ
                     GWVLKSLQYENTVGEMYEYKVGQETYKSSSIKDLVSVAYQNADKFKDMCTNKKEKGSF
                     MLGISTEFEI"
     gene            10111..10488
                     /locus_tag="PC545_002079"
     CDS             10111..10488
                     /locus_tag="PC545_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456846.1"
                     /translation="MINIDRRRKDIIRTININPTNITITSIKKTEIDGAFEETETEIK
                     CVVRIFNEKTAEKQISSEKQGTFSSIRTYGMLVSDDVVLDVNSRDSLEFECIYGRMKI
                     VNVYPQIVKGELCGYQCSLERID"
     gene            10490..10900
                     /locus_tag="PC545_002080"
     CDS             10490..10900
                     /locus_tag="PC545_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456847.1"
                     /translation="MSAFTNAINDINRKKAGMFVLCMSASAMLEGEAKANARWTDRTS
                     HARQSLNAKTLSGGNNFIIRLSHGAEYGGILEEGSKPHVITPKSAQALYWRGASHPVK
                     SVQHPGTKAMPIIKPTIDKNIGKIGNMIFRYWSD"
     gene            10901..11722
                     /locus_tag="PC545_002081"
     CDS             10901..11722
                     /locus_tag="PC545_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456848.1"
                     /translation="MRAGIRKALIDNIKELKGCYEPNVPNKDTKKPYMVVVQGQDNDN
                     GETIGFERSIEVWIYEGRTTFKKLDKLTKQVIEILDMNTIVDESENEAFTCIYKGTSE
                     NDIVVEEWDAIARGIRFSVIALEDKEDTTNDRWVEALSRHTKDLLEIESYKDNWKKNF
                     IAPCALWRTTHIENKRINYHLIEITKTMKCHVVSKNKDEIVKLLETLETSLIIDKRVR
                     LREDKNMYLTLVSVVEDRESDMFTTGQLTAVFKMIGKIKREGPTMDKIYGNGNLR"
     gene            11735..11938
                     /locus_tag="PC545_002082"
     CDS             11735..11938
                     /locus_tag="PC545_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456849.1"
                     /translation="MAETNNKKINVSKQEEKYLKSDFIENSEALGYKKEVVAGALFNC
                     KKEELTKSEFEKAIKEFLEREVK"
     gene            11940..13361
                     /locus_tag="PC545_002083"
     CDS             11940..13361
                     /locus_tag="PC545_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDF2456850.1"
                     /translation="MATGTWNEKEKKEIPGFYNRFKTQAEKSTNTGLKGRLAMPIRAN
                     WGEVGKVVTIKNDLRQLKTLFGDDMNYSAFKLGKLALLGNVKELLLYRLVDGNQKKGT
                     LTLKDTTENSAKDVIKLETKYPTSRNFNVTIKSNLVDADKKDFIFFEGTKQLFSSSVK
                     GTIDEIVLEINSNLDNEYVIATKVADSDTALANLVNVALEGGNDGCTSITNESYLKAL
                     EEFERYSFDSFVLDGVAEEALQETTKAWVAKNKELGKDILLFLGGKAEDNIKQINDKS
                     KSFNDENIVNVGSSAYYEGIKYTPSEVAVYIAALSVSKGITGSICNAKTIFEEVEPRL
                     SQSEVKECLKSGTLILDFDDGDVIIVDDVNTFKKYVDDKNEAIGYISNIMFINTINKD
                     TSLKRKEFVGKIFNDSTGQTTVICALKKYFEELMGQGIISEFNVDIDTELQATAKADE
                     FYWKWDAVKVDVMKKIFGTGYLG"
     gene            13377..13799
                     /locus_tag="PC545_002084"
     CDS             13377..13799
                     /locus_tag="PC545_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456851.1"
                     /translation="MGKYDENIIDAANVADGSNAKIIVDGKEEGYGTEFTAEVENDKK
                     TFRVIGCKWELNKASTQKGTFSLTVLKTTSEWIERGFDKFELISEIENPGLVGYERIR
                     YKNCMVDKIQLASIKADENIEIQIDGTFEGYELVDKIA"
     gene            13878..14351
                     /locus_tag="PC545_002085"
     CDS             13878..14351
                     /locus_tag="PC545_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456852.1"
                     /translation="MANLDKEFLNEGIEEERELTKEEIAKQQEDNIIMKLTEDAILPE
                     KTIFVKRLDIPLTLRALTEKEISALQKKYTKVTKVRGRRESKLMEDEFNIALIEKATI
                     VPNFCDARLLNSMNVSSGVEFIRRKFLAGEIALISDEVLELSGFYEELSDDDIKN"
     gene            14532..18860
                     /locus_tag="PC545_002086"
     CDS             14532..18860
                     /locus_tag="PC545_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDF2456853.1"
                     /translation="MREIAKKEMYHIDVVIDVTGDEQTKNKLSAMERYTKQTEKRMRA
                     LNRIKANPVIQAQDKTSSVVNRISNNLKRVGRTISTTINAKDRASSVVNRVKNKVNSL
                     LTSRQREVLLKARDKASQVVDKVKAKVQNLTAATIISLNMKADPALRVISQTRSKLGE
                     LKNNTIINIKAKGEEALNTISRTKSKLQEFSNRTYQAIVKLKDEASPTLSGLDGKISS
                     FISSTISKFTQLAVTATALIGGVGVGSAIKGFADFEQAMKNAQAVSSANSKEMAEMTA
                     MAREMGRTTSFTAKDAGNAMYFMGMAGWKSKEMIAGLPGILNLAATGQTDLALTADIV
                     TDGLTALGLTAKDTGMFVDVMAATVTNSNTDIERMGETFKYMGSVGGALGVSMKDLSL
                     ATGLMASASVKGSMAGTALRGGLVRLIKPPAEAQKAMNKYGIEIKKTKDGNLDLASTI
                     VDLREKLGGLEGVQKSAAISSIFGRTAMAGWAAVVNASEKDFKKLTTAINESEGEAKR
                     IADMKLDTLSGQFEILKSAIDDVRISVGQRLGPMTRSFVEQLTKDMPKIGDAIVSFVS
                     DFINDFDKIKSIMQNVISVISGIVSAFIAFKALKFISFLIPILSDIGFAIAAFAGGAA
                     TLGEALLFALGGPVGAVIAGVVLLATAFTLAYQKSDAFRKIVKNVGKSIKNFLQEAII
                     AISPFVNVFGNKLKELGKAVIPLLKVFGDFASTLMSKIGPIILFLSSNVLAGFILTFV
                     AAVEAVKSAVVAITGVLQGLTSIIKGVFDIVGGIISGDGKQIINGLKSVFEGGIKIVS
                     SVWKGLVDIVTSPIQAVVDVLDEKFGKKVEGIKKKWNELKDFLKNPTKAAPKVQPVNL
                     SGEKASNELQASSNGAKAYISSLGQKIGEGIGKIKDKFGELKISATEVFNNIVSFVGG
                     KATELKDKLLEGIKPAIDTFKQSLSNLKEVFGDSLDSIKEAFGGLKTVFDENIKTPFE
                     NLKQKVLETKESLKPVFDNLKSSFAELGKALEPIKEAFSGIKDFFSNLFKPIKDDGAT
                     KTTKTNMDELKQSTQRVGTSFKELGNAFNQLKEAAKPFIDYLKQIKDSLTSTLGDIGG
                     GLLKGVATSIVLVITSVINAIASIINAVAGAIKGVIDIIKGIFEIIGGIISGDGEKIK
                     QGFSDVFKGIGEVVKSLWEGIKGVLGAPLKAVVNFIDNGFSEKVGQVKQWWSDLKTNV
                     GQKISGFVSFVSNGFQQKVQQVGMWWQGLKVNLSGKISGFVSLVENGFKSKVDSIKSA
                     WDSLRKKLSQKITGFVSIVKTGISNVLDRFADGGVASKPSICGEAGPEMVIPLSNSKR
                     SRALSLYEQAGQMLGTKASNNVIPISQKLGTSFNSASSIQNSNSSIINNVRQFPTKQE
                     EFNNTENRIYQEAQPQNIISSGSNAINVGGISINIQGSNNKEEMIQEILSQVESGLRE
                     ALEDIG"
     gene            18940..19734
                     /locus_tag="PC545_002087"
     CDS             18940..19734
                     /locus_tag="PC545_002087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456854.1"
                     /translation="MWGRFKNMSIILKVLVLVCAIAIFPITLLAFSIEFVVKSFKRNE
                     RFKVVFGVFLVFITFSFVCIWYLLEDNITTSNSSNKKQEEHLKEEPGKNLATREAEEE
                     TNKKKEQEKQKNEGTEAEKKERDKTEKKEKVDKKYQEKNKEKAETKKQTLTSEELKKK
                     VDSIIPAEYKGNYYTNDVLDTDGSYVLSLQVQNASFDNESSCKAFTKDLINKLKEFRI
                     DSAEVYFVGSSGQTTYQINIDDFLKVQDNIKSVDNMEFFSFKDFKN"
     gene            19804..20475
                     /locus_tag="PC545_002088"
     CDS             19804..20475
                     /locus_tag="PC545_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDF2456855.1"
                     /translation="MVIDIYLKNEKEKIDFHFPVNPFDSLSIKKEKRFETVDIVNLGE
                     FDIKKEGEKIREISFKTFLPNLYDASYCRYSELKNPIEVVAMLEKWVDQAEPLRLIVT
                     GFGYNGLVTISNFSNTQTAGREEDRDIEITFRTYRELKIETLKKDTKSNTKTDLKDNR
                     PNTQTKSKIYTVKASDTLYKIAKNLLGKGSRWPEIYNIPENKKVIGKNPNIIKKGQKL
                     VIPSK"
     gene            20472..21434
                     /locus_tag="PC545_002089"
     CDS             20472..21434
                     /locus_tag="PC545_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ"
                     /protein_id="HDF2456856.1"
                     /translation="MKIILNGKYDIANFNEGITLSEAIDGVAYKMDVSLVEPKQLKDI
                     NIKKGDKIILIDIAYESKKEETIFDGVVWETRRSEKSKKLTLSCRERTVYMEESEEQY
                     QFKENTATQRIEYYCKQWNIPYYNLANTGKKLAKVIHKTNILDMIKKDLKETATKGGD
                     LFRVRMDNKLKLFKLGTNANVYKLDSILEDANFTSSFNDAVTSVKVLGKSKDENTKAP
                     IIGTYKKDADKFGTLQKIKQDEKIKNAKEAKKAAEAMFNSGEETISVDCAVDINRIRA
                     GDKVSLKSKEYYVIDVTHTLDSRPKMKLNIGSLEYIRRKFYNND"
     gene            21427..21792
                     /locus_tag="PC545_002090"
     CDS             21427..21792
                     /locus_tag="PC545_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456857.1"
                     /translation="MTDARFNGIARILKENMNKSVANGTFGMGCELAEITANGLKVNG
                     YKDEIQDYLVLENLTLKEDYFTFSDEALSGEYRHKHKIETPKELKPLRIGDNVLVAVM
                     GAEFVVIGRVVNAKPISSK"
     gene            21767..22225
                     /locus_tag="PC545_002091"
     CDS             21767..22225
                     /locus_tag="PC545_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HDF2456858.1"
                     /translation="MPNLFPQSETFETVELKNNDENELDLKGSFLFDFEKGEFVKNAD
                     GTLKKCDKVQAYKQWCQKAILTPRYKKAAYTNIYGSEIKDLIASNLSQSAKELEITRL
                     IKETILVHPYTKEVSNFIFVWLENSRLVNYEFDVLTIDDENITIDGNIKR"
     gene            22234..23370
                     /locus_tag="PC545_002092"
     CDS             22234..23370
                     /locus_tag="PC545_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDF2456859.1"
                     /translation="MERELPIPLFLTEEEDSVHERMLSNFQDVSTLEGDFIYDATRPT
                     AEQIAELKQLGLQNNLRIAFPQTSYGEYLEWLGECKGVFKNQPTKSVGMVTFNGAQGT
                     IITKGTIVTTIATDEKQSIEFELLETKTIGANETVDIKAECRIAGTIGNVSNNTITVL
                     LGSISGVKSVSNKEDFRGGTDIEDEEHFRERVLVAEQEDKLSGASSDYIRWAKEVDGV
                     GYAYVVPEWNGAGTVKVLILDKNRKAATQELIDKVQEYIYPLNISEGENRDGKAPIGA
                     LVTVVTPDTLLINVKASFIFSNGFSEETVLNNLKTKIDKYLDKIDLGGTVSYNAIQAI
                     VGSMMLTDEGIEDFSNLTINDVKENIKLQDQVVGIGEIVNEVVG"
     gene            23367..23996
                     /locus_tag="PC545_002093"
     CDS             23367..23996
                     /locus_tag="PC545_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HDF2456860.1"
                     /translation="MIASKKGKEMLLTLSPIYEQSIIMQSLYEAIGSEFDNLELLNKE
                     IELQLFPQSATWGLEFWENRVGLSTNIDEDIEARRRKVIAKLQSKYIMTPKRMAMILQ
                     SYTGANIKINENISPYTFGVELTSTQGFPKDLEDLYKRVNVIKPSHLAVSYKLVSLLK
                     SKTYFAQTAIMSEEITVYPYTSKEVKASVKAKFALAHNMSSETLTVYPR"
     gene            24009..>24664
                     /locus_tag="PC545_002094"
     CDS             24009..>24664
                     /locus_tag="PC545_002094"
                     /inference="COORDINATES: protein motif:HMM:NF023985.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2456861.1"
                     /translation="MADEQFYTILTNIGKAKIANAGMLGKSVILEKIQAGDGGGNYYN
                     PTEDQTALKNKVWEGNINAFDNDENNPNWIIATACIPGSIGGFTVREMGLIDNEGDMI
                     AVCKSPETYKPKVDNGAMKDLYLKFIIEVSNVEKVTLVVDPTAIFLTKKDEEKILTNI
                     NKLDTKIDTTKTELTSNIETTKTELNTRIDTENEKQNIKIDQLIAGGSNVASTQIITI
                     "
BASE COUNT        10152 a         2716 c         4844 g         6952 t
ORIGIN      
        1 gctatattta tgctagataa gaaacaagaa gtcttagaaa ctaaaataga gaatgttaat
       61 gagaagttag agaactttat ggatgatgca ccattattca atatcgagtg tgagtgtatt
      121 gttaaagagg ttaagagagt agcaacaaaa tcacttggtg gacatggaag taaggcttat
      181 aaaaataaat ctttaagagg taaagtttat agtgatatat accatcagat taaacgagaa
      241 tttggagtag atagttataa ggctataaag cgttgtcaat tagataaagt attagagatt
      301 gtaaatagtt ataagttacc tatagtgttt gaagaagaaa taagactttt gaatagtcaa
      361 ttatcaattg tgagttaaga caggaataga tttttctcct ttaaagggga aaacctgcaa
      421 tagttaatta aaagagaaaa ggagtgcttt cacactccac ttgtcaaaaa tataaagctt
      481 ttatccaaga ttattataac ataaacagga gtgtgaaagt atggataata atattaataa
      541 aaaagaacta tttaaaaaag tagaaggtag actacatcat tataaatttt taagtgcaga
      601 aattaaaaat cttgaattag atatagaaag tagagaaaat gaaatatttg gttgtaaggc
      661 tatcgggtat ggtgaaaaag taagtccaac atatgctttt aattcaactg ttgagaatga
      721 gattataaaa aaagaaagag atattgctag attaaaaaaa ctgaaaaaag ataaagaaat
      781 agaaaagaag aaaatagaaa atgcacttac atgcttagat ataagagaag aacatttttt
      841 taaactgttt tataatagca gaatgaaaaa tagtatggtt tatatatcct tagaaatgaa
      901 ctcagatagg aaaacatgta gatgtgtaag ggaaagatta gtgtataaag ttatggatat
      961 gctttatcca agaattaagg aaaatgaact cccattattt aaaaattaga aaattcccca
     1021 gtttttcccc agaaattccc actttattcc ctactttctc cccgttttga ttaaaaaagc
     1081 atgagataat agtattgtgg aaataaagat ttccctctca aaacttaata tttgactagg
     1141 gttaagggat tgccctagtc actatgaaca gactaggcag ggcatgagga tgctgttagt
     1201 tcaattctaa ctatgttcaa tctttagttt ttctatttca attaatctac ggatacacta
     1261 aaaaatagta tatgaattga gattaaaatc tcatacaatt ttgtatctta attcaaagac
     1321 taaaaaccga gtggggcttg gtaacctcac tcaccatgca agtactggtt taatctaggt
     1381 tcgattcctg gaacttgctc cctttaataa tatgtatccc ccattaaaaa ggcttagatt
     1441 aacttctagg tcttttttaa tacaaaaatt tattaaaaat gcacggcatg cactattcag
     1501 atatgcatgt catgcatgtc taaagtcgaa tatttaatat tttcgatggc tcaaattgag
     1561 gggtcgaaaa taaaaacagg aggtagtagt atgttgaaaa ttttacaaga gaaaaatgta
     1621 aaagtaatgt ggtccaaaaa tggagaagaa gtttggttta atgcaaatga cgtaggagag
     1681 gaactaggca tagtaaatat tcgtgataca ttaagaaata tagatagaga atataaaaag
     1741 aaatttaatg agtctactgt cggagattcc tacactagaa actttaaaga taaattgcct
     1801 aacttcggta ctacttttgt tacagaagaa gctgtgtaca atatgtcatt tagaagtaat
     1861 aaaccagaag caaagttatt tacaaaatgg gttacaaaaa cacttaaaca aattagaata
     1921 catggttatt atattgctac agaaaaagac caggaatggc tggatataag gacagaaggc
     1981 aaaaaagtaa gaaaagattt tacagatgaa atacaagagt ttgtatatta tgctactagt
     2041 caaggtagca ataaacctca gatgtattat aaacatttta ctgaacttgt aagaaaaaaa
     2101 ttaggtattc caaaaggtgt gaaaagagat gagttaaatc aaagcgaact atttgatata
     2161 caagcacttg aaagaattat atctatgaaa ttacctaagt taatagataa agatatgaat
     2221 tataaagagg tatataaaaa gattaaggaa ttaatagaaa tgatttaagg gactgtcttg
     2281 atggagggtc ttttttaatt cccaaaacga caaacaaacg aggtggtgat gtgcaagatg
     2341 tcaaagaaaa ggtaaaacaa gattacataa aaggtatgaa acaaaaggaa atatcagcaa
     2401 agtatgacat tagtttaaac actttaaagt catggataaa aagatacaat tgggctagtg
     2461 agaaaaagaa gggtgcacct aaaaataaaa gaggtgcacc cataggtaat aaaaatgcca
     2521 ctggtcctcc tggaaataaa aatgctgaaa agtttggttt cttctcaaaa tatctacctg
     2581 aagaaactag ggaattaata caagaaatat ctataaaaga taaatttgat attctttggg
     2641 aacagataac aattcaatat gcagcaataa taagagcaca aaagataatg tatgttaaag
     2701 acaaggaaga aatgattaag gagttaaaga aacatgaaag cacagaaaat ggtgagaaga
     2761 tagagtatga atttcaattt gcatgggata ggcaagcatc ttttcttaat gcacagagta
     2821 gggctatgag tgagttaagg agtttaatta aacagtatga tgaaatgatt cataaggatt
     2881 ggaatttagc tacagaggag cagaaaaata gagttgaaaa gttaaaatgt gaagttgata
     2941 acctaaagaa aagtgatact ggagatgatt caaaaatttg ggttgaagct atacaaaata
     3001 ttgcaatgaa acgtggtgtt aacaatggat aaagctttat tgacactatt agattgttat
     3061 tgggataatc ctgtttggtt tgcagaggat atgttaaatt ttaaagctga caagtggcaa
     3121 tctgatgttc tgatggcttt agctcaaacc ccaaaagtat ctattagaag tggtcaagga
     3181 gtaggtaaaa ctggattaga aagcattgca actgtatggt atttaagcac tagacctttt
     3241 ccgaaagtag ttgctacagc tccaacacga caacaattat atgacgtact atgggctgaa
     3301 atagctaaat ggctaagtaa tagcaaggtt gagaagctac ttgagtggac taaaacaaaa
     3361 gtgtatatga aaggctttga agaaagatgg tgggctacag ctagaacagc agtaaagccc
     3421 gagaatatgc aaggttttca tgaagattat atgttatttg ttgttgatga agcttcggga
     3481 gttgctgacc ccattatgga agctatattg ggaacattat caggtgcaga aaataagctt
     3541 cttttatgcg gaaacccaac tagaacgagt ggaacgtttt acgatagcca taatagagac
     3601 agagatttat ataaaacatt taaagtatct tctttagata gccctagaac atcaaaagat
     3661 aatattgaaa tgctaaaaag aaagtaccat gaaggttctg acccttggcg tgttagagtt
     3721 cttggagaat ttccaaaagg tgaaagtgat tctttaatat ctttagaagc tgttgaaaca
     3781 agcacaataa gagaagtgaa tatatctaat gactatatat taaatatagg tgcagatata
     3841 gcaagatatg gtgacgatga aactataata gctccacgaa taggtggaaa agtgtttgat
     3901 ttattaactt attctaaaaa agacacaatg gaaactgtag gaaatatatt aagagcagtt
     3961 gataaattta aaagcatgta tcatcaaatc aacagagtaa aaataaaaac ggatgatgat
     4021 ggtttgggtg caggtgttac agacagatta aaagaggtta taagacaaga aagactcaaa
     4081 tatgaaattg tacctattca aaatggttct agtgctatag aaaaagataa atactataat
     4141 aaagcttctg aaatgtggga taacatgagg gaggaattag atgcaaattt aagtggcttc
     4201 atacaaaata aagaagctat aatacagctt cctaatgatg ataaacttat taagcaacta
     4261 tcaaatagaa aatatacagt agattcaaaa gggaaaatac aaatagaaag taaaaaggaa
     4321 atgaaaaaaa gaattggaga atcacccgat agagctgatg cagtaatata ttcgtttgca
     4381 gaaaataaca atactgattt atctttactg aaagggggta gtgtatgggg ataatatctt
     4441 atgtaaaaaa gctatttaaa agacctactg gagaaattat gcgtatgtct agtggaaata
     4501 taggcgtata taaattagat gattctagag ttgattatga gttagcaaga gaattgtatc
     4561 aaaacaaaaa tgctaattat aaattaggtt ctagttttgt tagaccaatt gtcaattcaa
     4621 caactggttt tatgggtgta cctcattttc aaatagaaga tgaagaagct caatatatat
     4681 tagatgaatt tgttttagat aacacatcta aaatgttaaa aacacacaca gatagtttaa
     4741 agcaaggtga ttgttatatt tggataacta gagaagaaag agaaaatcct ttatatcctg
     4801 ataaaaaagt tagattaata tataacttca tatcacccga agaagtgaaa gaaataatat
     4861 tagaccctac gacaaaagag cctatagctt atatattaga aagccaaaat gaatggactg
     4921 acttagaaga aaataagaga agggctaagg taaaacaaat aataactgct gaaagtagaa
     4981 ttattgaggt tgaaggggat aagatagaag gtttagaaga aggggaaact cctaatgtat
     5041 ggggctttat accaataata cattttaaaa atgaagctga tgaaacattg aaatatggtc
     5101 aaagtgatat agaaccaata gaacctcttt taaaagctta tcatgatgtt atgttacatg
     5161 cgttaaaagg tagcaaaatg cactctactc caaaactaaa gctgaaatta actgatgttg
     5221 caagtttctt agcacacaac tttggtgttg aagacccagt taaatttgca aaagagggtg
     5281 gaaagataaa tcttgatgga catgaaatac tattcttaaa taaagatgaa gaagctgagt
     5341 ttgtagaagt aaaatcagct ataggtgatg ctaaggagct tttaaagctt cttttttatt
     5401 gcatagtaga tgtatctgaa acacccgagt ttatatttgg agtacataca cctagtgctt
     5461 tagcttctgt aaaagaacaa atgcctatta tggtaaataa gataagaaga aaaagagaac
     5521 aatttacaaa tagctggcaa ttacttgcaa gaatggtttt aataatgagt tctaattcta
     5581 gtggtatgaa atattcatct tatgatgtga ctataggttg ggatgaagta aatccgcgtg
     5641 atgataaaga attagctgaa acactagaaa aggtatgtag tgcattagat aaagctttag
     5701 aaggtggatt tattagcgaa gaatcaacag taaacttttt agctcagtat atagatacaa
     5761 tgagcaatta tataagtgat gaccctgaaa tagttggaga aagagaaaag ataataaaaa
     5821 ctaaaatgtt aaaatacaga ttagatgatt ctcaaggttt agatgatgag tcaaatgaaa
     5881 ttgacaagga aatagataaa ataaaggata ataatgacaa tggataaaag tactttggaa
     5941 ttaataactg ttgcagggga gtacaagaaa tgggcattag aagctagaaa aaaatttata
     6001 gatttaagac tcaagcaaga tgatgaaata agaacaatgt atattaacat aacaagaaat
     6061 attacaaaag aaataagaaa aggaaatctt tcagacttta acaaagttag gttaaaacag
     6121 atactaaaac aattaacaca agaaataaaa atattaaatg aacaactagt atttaatttt
     6181 gatgaatact taaataaaaa tgttgaaaca gctactagtt actctaaaaa tattttaatt
     6241 aatgcagttg agacagctca aataactaaa gtaactaaag ctatgataca aaaagctttc
     6301 tatgatatta atataagaac tgtagaagct tattatacaa gggttaagga tggtttattt
     6361 ttatctgata gaatttggtc taagtgtaag aagtacagag aagatatgaa agttatatta
     6421 caaacagcag taacagaagg tcaagactgt gttaaaacag ctaagatgct agacaaatac
     6481 gttttaaaag gtaagaaaac tttagttgac gaatatccaa atatgataaa aagaatagga
     6541 aatagagtac ctcaaaatat aagttatgaa gctttaagat tggcaagaac tgaaatgaca
     6601 tcagcttatg gtgatggggt cttagcttct gcaatgatta accctgcaac cataggtatt
     6661 cagtttatgt tgtccatggc acatcctcat acagatatat gcgacgaaat atgtggagag
     6721 gataattttg gtttgggtaa aggtgtttat cctataaatg aagctcctgc atatccattc
     6781 caccctcatt gtttgtgtat tatgcttact gtagttcaac cattagatat attagttgga
     6841 aggttgaaaa attggattaa aaatcctatg aatgatacac ctcttgaaat gtggtatcaa
     6901 gaggtgtatg gaaatttgaa tttttaaatt gaaaggtggt gattaaatga atgtaataac
     6961 tggagaaatg gactcaatga atgcgttaat atctagtata aaaccttctg atattccttt
     7021 agctaaagat atagacatag aagctttaaa atctatagat gatgaccctc ttgaggtagt
     7081 tgttgagata ccagctacaa aatctaaaag gggatggaac tatactgcta aaagcttgaa
     7141 agatattgta gattacacta atgaaaatac tcttaatggt tttttaggac atcaaaaagc
     7201 tgaaaatata tcaactgaat ttgcaccacc tgtaacgcat tggataggtg cagaaatgaa
     7261 aggggataaa gcttatttca gaggactgat tgatgctgat gcaacaaact tgaaaagatg
     7321 gattagaact aaaaggataa aagaagttag tatatttggt tatccaaaac ttaaaaagag
     7381 tgctaaaggc gaaatgaatg ttataggata tgagccacta tctattgatt ggactcctct
     7441 acatagacca ggtatgccaa caagtattgt aggtatggaa atgagtccta atggcgaaca
     7501 gttagatgga acttttgaag ctttaagaat agatttaaga gaagctttaa aagctaagtt
     7561 ttctattaat gataataatt catatctcta tatacaaaac ataagatatg ataacaatac
     7621 tgtcatatat gagttggagc aaaatggatt atgcaagctt tatagtatac catttactat
     7681 agttgaaaat aaaataaatc taggtgaaga aattgaagta ataaagaaaa taagctatga
     7741 agctaaagga gaaatgaaag gagaggaaaa caaattggaa ggaaaagagt taataaaaaa
     7801 tgtcaaagga ttactgcaaa ctggtgaaat atcatattca gaggtcatac aaggaatagg
     7861 cttaactaag gaaattgtga caggagagat ggaagatgta aaaagttcat taaaagcaga
     7921 aaaagaatta agagaagtga aaaaagtact tggaatagta ggagagatgg acacagttga
     7981 agtggcaaaa aaggcttcaa aagctttaga aaatgagaaa aaggaagctt ggaactgtat
     8041 agttaataaa gtaattaaag ataaagtgtc aggtgaaata gctcaaacat tagttaagaa
     8101 aatgttaaat gttgaggaag gctcaagtga agaagtaata acaggagaaa tagaaaatat
     8161 attaaatgat gagtttgtaa aaaatacaat gtctaatatg tataaagata atccaacaac
     8221 aacaggatta ttaaactcta gcaataatgg aagtttaaca actaagaaaa atagaatata
     8281 aaggagtgat gtttatatgg catttaaagg tcaaccaacg ccaagcacaa taacacagat
     8341 aacaagagca aaaataagtg atgggaaatc tgtaagagtt attctttcag aaggtgaaag
     8401 cactaaaaca caacaatttt atcttataaa tggattcttt ggagtcgcta tgcaagacgg
     8461 agaaaaaggc gatgaagtta ctttgcaaat agagcaagct gaatacgaaa cggataatat
     8521 tgttacatca gaagcttttg aggcagggaa attgatttat tgggataata cagctaagaa
     8581 atttactact acatctgcaa gtaataggct agttggtaga gtaacagatg ggaaagacag
     8641 taataatgta atttggttta tattattacc tcaacaatag aaaaggagtg ataaatatat
     8701 ggcatttaaa gtaattagtc aggaaaattt gctggaacaa aaaagaaaag aaactttaca
     8761 agaagatata ccatttatag taaatggtga aatggaatat gtaacaaaga aaatatcaaa
     8821 tggagaaatg gaaaccttgg agttaaataa gccacttggt gaaatgatga cttttagctc
     8881 gacttcaaat ttaaaagagt tattaagaaa agttgtatta gatgttgaac taggcagaga
     8941 gcaagtacaa ctattatata aaccaatcta tgacagtata gcagattcta atttaccaca
     9001 agttatggat gctaagtggg ctttacaagg taactgtgta ttcctagagc atatagaagg
     9061 tgaagaaatt aaattcggta caataaatgc agaaaatggt ccagttgcaa ggatacaaac
     9121 ttatgcaact ggttttgagt atacaaaaga aatgaaggat tttaaccaaa catttagtgt
     9181 tgaaatatta aataaatcaa ttggtgagag ttacaatgcc ttgttaaacc acatacatct
     9241 aagcccaata ataaatttta attataaagc ttctaataag acagctttta aaggtgaaac
     9301 taatgaccca atatggctag gaatttggag aacattaaca caagcacaaa aagatacagt
     9361 tatagcaaaa agacaaggta atatattaat ggcttctagt gctgaccaaa ttgaaataga
     9421 aatggcgtta aatggaggac atttattaaa cggaagcatg tatccatcta taaaaaatat
     9481 atcaacagta atttattatg atgggtggga ggttactgtt ggtaaaaaaa catattctta
     9541 caaaggtgtt acaccaggca aaggatattt gataagacct aagcgaggat ttaaagagtt
     9601 aataaagaga gatttaacaa cagaggttgg aaatgctgat ttaagtaagt tagtagaaaa
     9661 tcaaattgta ggtcattgtt atagaggtgc ttttgcagca gtagaagaaa atgtacaaga
     9721 aataagtttt agataaaaca ctcataagag tgttattttt atgaggtgat aatatatgac
     9781 accaactagt aatttaatag aaaaattaag actattatta aatgataaag ataaaaaatc
     9841 atttacagat gaagaattaa acttgttttt agaagaagca gactgtattt actgtgcagc
     9901 ttctcaagga tgggtattaa aatctttaca atatgaaaat acagtagggg aaatgtatga
     9961 gtataaagtg ggtcaagaaa catataaaag ctctagtata aaggacctag tatctgtagc
    10021 ttatcaaaat gcagataaat ttaaggatat gtgtactaac aaaaaagaaa agggaagttt
    10081 tatgttagga attagcacag aatttgaaat atgataaata ttgatagaag aagaaaagat
    10141 ataataagaa ctattaatat aaaccccact aatattacta taactagtat taaaaaaacc
    10201 gaaatagatg gagcttttga agaaactgaa acagaaataa aatgtgttgt tagaatattt
    10261 aacgaaaaga cagcagagaa gcaaatatca agtgaaaagc aaggtacatt tagttctatt
    10321 agaacatatg gaatgttagt aagtgatgat gttgtcttag atgttaacag tagagattct
    10381 ttggagtttg agtgcatata tgggagaatg aaaatagtta atgtatatcc tcaaattgta
    10441 aaaggagaac tttgtgggta tcaatgttca cttgaaagga ttgattaaaa tgagtgcttt
    10501 cacaaatgca ataaatgata taaatagaaa aaaagcaggt atgtttgtac tttgtatgag
    10561 tgcaagtgca atgctagaag gtgaagctaa agcaaatgca cgttggacag atagaacatc
    10621 acatgcaaga caaagtttaa atgctaaaac acttagtgga ggaaataatt ttatcattag
    10681 attatctcat ggtgcagaat atggagggat acttgaagaa ggctcaaaac cacatgttat
    10741 tactccaaaa tcagctcaag ccctatactg gagaggtgct tcacatcctg taaaatcagt
    10801 tcaacatcct ggtacaaaag caatgcctat tataaaacca actattgata aaaatatagg
    10861 caaaataggt aatatgattt ttagatattg gagtgattaa atgagggcag gaataagaaa
    10921 agctttaata gataatataa aagaattgaa aggttgttat gaacctaatg taccaaacaa
    10981 agatactaaa aagccttata tggtagttgt acaagggcaa gacaatgaca atggagaaac
    11041 gataggtttt gaaagaagta tagaagtatg gatttatgaa ggtagaacta catttaagaa
    11101 gttagataaa ttaactaaac aagttattga aatcttagac atgaatacta tagttgatga
    11161 atctgaaaat gaagctttta cttgcattta taaaggtaca agtgaaaatg atattgttgt
    11221 tgaggaatgg gatgctatag caagaggtat aaggtttagt gtaatagctt tagaagataa
    11281 agaagataca actaatgata ggtgggtaga agctctatct aggcacacaa aggatttatt
    11341 agaaatagag agttataaag ataattggaa gaaaaacttt atagctccat gtgcattatg
    11401 gcgaactaca catattgaaa ataaaagaat taactatcat ttaattgaga ttactaaaac
    11461 tatgaaatgt catgttgtaa gtaaaaataa agatgaaata gttaagcttc ttgaaacatt
    11521 agaaacaagc ttaataatag ataaaagagt aagacttaga gaagataaga atatgtattt
    11581 aactcttgtt agcgtagttg aggataggga atcagatatg tttacaactg gacaattaac
    11641 agctgtgttt aaaatgatag gaaagataaa aagagaaggt cctactatgg acaaaattta
    11701 tggtaatgga aatttaagat aggaggtgta agggttggct gaaacaaata ataaaaagat
    11761 taatgtgagt aagcaggaag aaaaatattt aaaaagtgat tttatagaaa atagcgaagc
    11821 acttggctac aagaaagaag tagttgcagg tgctttattt aattgtaaga aagaagaact
    11881 tacaaagtca gagtttgaga aagcaataaa agagttttta gaaagagagg tgaagtaaaa
    11941 tggcaactgg tacatggaat gaaaaagaaa aaaaggagat acctggtttt tacaatcgct
    12001 ttaaaacaca agcagaaaag tctacaaaca caggattaaa gggtagatta gcaatgccta
    12061 ttagggctaa ttggggagaa gttggcaagg ttgtaacaat aaaaaatgat ttgagacaac
    12121 ttaaaactct atttggtgat gatatgaatt attcagcttt caagctagga aaactagctt
    12181 tactaggtaa tgtaaaggaa ttacttttat ataggctcgt agatgggaac caaaaaaaag
    12241 gtacattaac actaaaagat actacagaga atagtgcaaa agatgtaatt aagctagaaa
    12301 ctaagtatcc aactagcaga aattttaatg taacaataaa atctaatcta gtagatgcag
    12361 ataaaaaaga ctttatattc tttgaaggaa ctaaacaatt atttagttca agtgtcaaag
    12421 ggactataga tgaaatagta ctagaaataa actcaaattt agataatgag tatgtaattg
    12481 caactaaagt agctgatagt gatacagcac tagcaaactt ggtaaatgta gcacttgaag
    12541 gtggtaacga tggttgcaca tctattacta atgagtctta tctaaaagca ctagaagaat
    12601 ttgaaagata tagttttgac tcttttgtac ttgatggtgt ggctgaggaa gcattgcagg
    12661 aaactacaaa agcttgggta gctaagaata aggagctagg aaaagatata ttattatttc
    12721 ttggtggaaa agcagaggat aatataaaac aaattaatga taaatcaaaa agttttaatg
    12781 atgaaaatat agttaatgtt gggagttcag cttattatga gggaataaaa tatacaccta
    12841 gtgaagtagc tgtttatatt gctgctcttt ctgtaagtaa aggtataaca ggaagtatat
    12901 gtaatgctaa gactatattt gaagaagtag aaccaagatt aagccaaagt gaagtaaaag
    12961 agtgtttgaa atcaggtaca ctaatacttg attttgatga tggagacgta attatagttg
    13021 atgatgtaaa cacatttaag aagtatgtag atgataagaa tgaagctata ggatatatct
    13081 ctaatattat gtttattaat actataaata aagatacttc attgaagcga aaggaatttg
    13141 taggtaagat atttaatgat tcaacagggc agacaactgt tatatgtgca ttaaagaaat
    13201 attttgaaga attaatgggt caaggcatta tatcagaatt taatgttgat atagatacag
    13261 agcttcaagc aactgctaaa gcagatgagt tttattggaa atgggatgct gtcaaggttg
    13321 atgtgatgaa gaaaatcttt ggaactggat atttaggata aaggaggtta taaagtatgg
    13381 gaaaatatga tgaaaatatt atagatgctg caaatgttgc agatggctcg aatgctaaaa
    13441 taatagttga tgggaaagaa gaaggatatg gaacagagtt tacagctgag gttgaaaatg
    13501 ataagaaaac ctttagagtg attggttgca aatgggagct taacaaggca tctactcaaa
    13561 aaggtacttt ttctttaact gtacttaaaa caacatctga atggatcgaa agaggatttg
    13621 ataaatttga attaatatct gaaatagaaa atcctggatt agttggatat gaaagaatta
    13681 gatataaaaa ttgtatggta gataaaatac aactagcaag tataaaagct gatgaaaata
    13741 tagaaataca aatagatggt acttttgagg gttatgagct agtggataaa atagcataat
    13801 aaataaattt aagctacatg taattaattt tatatgtagc ttttcaaata aaactataaa
    13861 aatggaggaa gttaaaaatg gcaaacttag ataaagaatt tttaaatgaa ggaatagaag
    13921 aagaaagaga acttactaaa gaggaaatag caaagcaaca agaagataat ataattatga
    13981 aattgacaga ggatgctata ttacctgaaa aaactatttt tgtaaaaaga ttagatatac
    14041 cacttacact tagggcttta acagaaaaag agataagtgc gttgcaaaaa aaatatacaa
    14101 aagttactaa ggtaagaggt agaagggaaa gcaaactaat ggaagatgaa tttaatatag
    14161 ctctaataga aaaagctaca atagttccta atttttgcga tgcaagactt cttaattcta
    14221 tgaatgtatc tagtggtgtt gaatttataa gaagaaagtt cttagcagga gaaatcgcat
    14281 taattagtga tgaggtacta gaattgtctg gattttatga agaattaagt gatgatgata
    14341 taaaaaacta ataaagagag gtgggaagat tactatttta tacaacgcat atgttaaaca
    14401 tagtattctt ccagaggact ttttaaaaag agagaaaaca cctcaacagc ttcttagagt
    14461 ttttactcaa catgaaatag aacaagaaaa taaagctatg aaaaataaat aaaatttaaa
    14521 ctaaaagtga ggtgagagaa atagctaaaa aagaaatgta tcacattgat gttgtcattg
    14581 atgttacagg agatgaacaa actaaaaata aattaagtgc tatggaaaga tacacgaaac
    14641 agacagaaaa gagaatgaga gcactaaata ggataaaagc taatccagtt atacaagctc
    14701 aagataaaac atctagtgtt gtaaatagaa ttagcaacaa cttaaaaaga gtgggtagaa
    14761 ctatatctac aaccataaac gcaaaagata gagcatctag cgttgtaaat agagttaaaa
    14821 acaaagtaaa tagcttactt acaagtcgac aaagagaagt tttattaaag gctagagaca
    14881 aagctagtca agttgtagat aaagtaaaag ctaaggtaca aaatttgact gcggctacaa
    14941 taattagctt gaatatgaaa gctgacccag cactaagagt tatttctcaa actagaagta
    15001 agttaggaga actcaaaaac aacacaataa taaatattaa agcaaaaggt gaagaagcat
    15061 taaatactat ttctcgtact aagagtaaat tacaagagtt ttctaatagg acttatcaag
    15121 caattgtaaa attaaaagat gaagctagtc caactttgag tgggttagat ggtaagataa
    15181 gttcttttat aagtagtact attagtaagt ttacacaatt agcagtaaca gccacagcat
    15241 taattggtgg tgttggagta ggaagtgcta taaaaggatt tgcagacttt gaacaagcaa
    15301 tgaaaaacgc acaagctgta tcaagtgcaa attcaaagga aatggcagaa atgactgcaa
    15361 tggcaaggga aatgggtcgt acaactagct ttacagctaa agatgcaggg aatgctatgt
    15421 attttatggg gatggctgga tggaaaagca aagaaatgat tgctggtcta cctggtatat
    15481 taaatttggc agcaacaggt cagacagatt tagcactaac agcagatatt gtgactgatg
    15541 gtttaactgc tttagggcta actgcaaagg atacaggaat gtttgttgat gttatggcag
    15601 caacagttac aaactctaac acagacatag aaagaatggg tgaaactttt aagtatatgg
    15661 gaagtgttgg aggagcatta ggtgtttcta tgaaagattt gagtttagca actggcttaa
    15721 tggctagtgc aagcgttaaa ggtagcatgg caggtactgc acttagaggt ggtttagtta
    15781 gattaataaa acccccagct gaggcacaga aagctatgaa taaatatgga atagaaataa
    15841 agaaaacaaa agatggaaat ttagatttag ctagtacaat cgttgacctt agagaaaaat
    15901 taggcggact tgaaggagta caaaaaagtg ctgcaataag tagcatattt gggcgaacag
    15961 ccatggccgg ttgggcggct gtagtaaacg caagtgaaaa agattttaaa aaattaacaa
    16021 cagctataaa tgaaagtgaa ggagaagcta agagaattgc tgatatgaaa ctagataccc
    16081 tgtcaggaca atttgaaatt ttaaaaagtg ctattgatga tgtaagaata tcagtaggtc
    16141 aaagacttgg acctatgaca cgaagttttg ttgaacagtt aacaaaagat atgcctaaaa
    16201 taggggatgc catcgttagt tttgtaagtg attttataaa tgattttgat aaaattaaaa
    16261 gtattatgca aaatgtcatt tcagtaatat ctggaatagt atctgcattt attgctttca
    16321 aagctttaaa gtttatttct tttctaattc ctatattaag cgatatagga tttgcaatag
    16381 ctgcttttgc aggtggtgcg gcaacgctag gagaagcttt attgtttgca ctaggtggac
    16441 ctgttggtgc tgttatagca ggagtagtat tactagcaac agcatttaca ttagcttatc
    16501 aaaaatcaga tgcttttaga aaaattgtca aaaatgtagg aaaatcaatt aaaaattttt
    16561 tacaagaagc aataatagca atttcacctt ttgtaaatgt gtttggtaac aaattaaaag
    16621 aattagggaa agctgtaatt ccattgttaa aagtatttgg ggattttgca tcaacgctaa
    16681 tgagtaaaat cggacctata attttgtttt tatcaagtaa tgttttagct ggctttatat
    16741 taacttttgt agctgctgta gaagctgtta aatctgctgt agtcgcgata acaggtgtat
    16801 tgcaaggttt aacatcaatt ataaaaggag tttttgatat tgttggagga ataataagtg
    16861 gtgatgggaa acaaataata aatggcttaa aatctgtatt tgaaggagga ataaaaattg
    16921 tttcttctgt ttggaaagga ttagtagata ttgtaacttc tcctattcaa gctgttgtag
    16981 atgttctaga cgaaaaattc ggaaaaaaag tagagggaat aaagaaaaaa tggaatgaat
    17041 taaaagactt cttaaaaaat cctactaaag cagctccaaa agttcaacct gtcaatttat
    17101 ctggagagaa agcatcaaac gagttacaag cttcatcaaa cggagcaaaa gcatatataa
    17161 gttcgttagg tcaaaaaata ggtgaaggta ttggaaaaat taaagataaa tttggagaac
    17221 tcaaaatatc tgcaacagaa gtatttaata atatagtatc tttcgtagga ggcaaagcaa
    17281 ccgaattaaa agataaactt ttagaaggca taaaacctgc tatagataca tttaaacaat
    17341 cattaagtaa tttaaaagaa gtttttggag attctttaga tagcataaaa gaagcttttg
    17401 gaggtttaaa aactgtattt gatgaaaata ttaaaacgcc tttcgaaaat ttaaaacaaa
    17461 aagttttaga aacaaaagaa agtttaaaac cagtttttga taacttaaaa tctagctttg
    17521 cagaactagg aaaagctctt gaaccaataa aagaagcatt tagtggaata aaagatttct
    17581 tttcaaattt gtttaagcca attaaagatg atggagcaac taagacaact aaaaccaata
    17641 tggatgagtt aaaacaatca acacaacgtg ttggaacatc tttcaaagag ttaggaaatg
    17701 ctttcaacca attaaaagaa gcagcaaaac cttttataga ctatttaaaa cagataaaag
    17761 attctttaac atctactctg ggagatatag ggggaggatt actcaaaggt gtagcaactt
    17821 ctatagtttt agttataact tctgttatta atgcaattgc atctattata aatgctgtag
    17881 caggtgctat aaaaggtgta attgatataa taaaaggaat attcgaaatc ataggtggga
    17941 taattagtgg tgatggtgaa aaaataaaac aaggattttc tgatgttttc aaaggaattg
    18001 gggaagtagt caaatcattg tgggaaggta taaaaggagt tttaggagca ccacttaaag
    18061 cagttgtaaa ttttattgat aatggatttt cagaaaaagt agggcaagta aaacaatggt
    18121 ggtctgattt aaaaactaat gtaggccaaa aaataagcgg atttgttagt tttgtaagca
    18181 atggttttca acaaaaagtt caacaagttg gaatgtggtg gcaaggactt aaagtaaatt
    18241 tatctggcaa aataagtgga tttgtaagtc ttgtagaaaa cggatttaaa agtaaagtgg
    18301 attcaattaa atcagcatgg gatagtttaa gaaaaaaatt atcacaaaaa ataactggtt
    18361 ttgtaagtat agtaaaaact ggaataagta atgtattgga ccgttttgct gatggtggag
    18421 ttgcaagtaa accaagtatt tgtggagaag caggtcctga aatggttatt cctctttcta
    18481 atagtaagag aagtagagca ttaagtttat atgaacaagc aggacagatg cttggaacta
    18541 aagcaagcaa taatgttatt ccaatatctc aaaaattagg aactagtttt aattctgcaa
    18601 gtagtatcca aaatagtaat tctagtatta ttaataatgt tagacaattt cctaccaaac
    18661 aagaagaatt taataataca gaaaatagaa tttatcaaga agctcaacca caaaacataa
    18721 tttctagtgg aagtaatgcg attaatgttg gtggaatatc tataaatatt caaggtagca
    18781 acaacaaaga agaaatgata caagaaatat tgtctcaagt ggaaagtgga ttaagagaag
    18841 cattagaaga cattggataa tgtcgaattg ttgttaaaaa aatcctcctt atagatgtta
    18901 taatttaatt ataaattaca tgagggggat ttgcatatta tgtggggaag atttaaaaat
    18961 atgagtataa tcttaaaggt tttagtttta gtttgtgcta tagcaatttt tccaataact
    19021 ttattagcat tttctataga atttgtagtt aaatctttta aaagaaacga aaggtttaaa
    19081 gttgtttttg gcgtattttt agtttttatt acattttcat ttgtatgtat ttggtatttg
    19141 ttagaagata atataacaac tagcaatagt agtaataaaa aacaagaaga acatctaaaa
    19201 gaagagccag gtaaaaatct agcaacaaga gaagcagaag aagaaacaaa taagaagaaa
    19261 gaacaagaga aacagaaaaa tgaaggtaca gaagctgaaa aaaaagagcg agataaaact
    19321 gagaaaaaag aaaaagtaga taaaaaatat caagagaaga ataaagagaa agcagaaacg
    19381 aaaaagcaaa cattaactag tgaagaatta aagaaaaaag ttgattctat aattccagca
    19441 gagtataaag gaaattatta cacaaatgat gtattagata cagatggtag ctatgtactt
    19501 agtttacaag ttcaaaatgc aagttttgac aatgaaagca gttgtaaggc atttactaaa
    19561 gacttaatta acaagttgaa agaatttaga atagactcag cagaagtata ttttgtaggt
    19621 tcaagtggtc aaacaacata tcaaattaat atagatgatt tcttgaaagt tcaagataat
    19681 attaagagtg tagataatat ggagtttttc tctttcaaag attttaaaaa ttagatatat
    19741 aagaaaaaga cgtttattta ttaggaatag atgtcttttt tattgtaaaa aggaagtgat
    19801 aatttggtaa tagacatata cctaaaaaat gaaaaagaaa aaatagattt tcattttcca
    19861 gtaaatccat tcgatagttt atctattaaa aaagaaaaaa ggtttgaaac tgtagatata
    19921 gtaaacttag gcgaatttga cattaaaaaa gaaggagaga agataagaga aatatcattt
    19981 aaaacatttc tgcctaactt atatgacgct tcttattgca gatacagtga gttaaaaaat
    20041 ccaatcgaag tagtggcaat gcttgaaaag tgggtagacc aagctgaacc attacgatta
    20101 atagtaactg gttttggcta caatggatta gttacaatat ctaattttag taatactcaa
    20161 acagcaggaa gagaagaaga cagagatatt gagataacat ttagaactta cagagaactg
    20221 aagatagaga cattaaaaaa agatacaaaa agtaatacta aaacagattt aaaagataat
    20281 agacctaata cccaaactaa atctaaaata tatactgtta aagcaagtga tacattatat
    20341 aagatagcta aaaatctttt aggtaagggt tcaaggtggc cagagattta taatataccc
    20401 gaaaacaaaa aagtcattgg taaaaatcct aatataatta aaaaaggtca aaagttggtg
    20461 atacctagta aatgaaaata atattaaacg ggaaatatga tattgcaaat ttcaatgaag
    20521 gaataactct ttcagaagct atagacggag ttgcatataa gatggatgta tcattagtag
    20581 aacctaaaca acttaaagat ataaatatta aaaaaggtga taaaataatt ctaattgata
    20641 tagcatatga gagtaaaaaa gaagagacaa tatttgatgg agttgtatgg gaaactagaa
    20701 ggagtgaaaa gagtaagaaa ctaacattgt cttgcagaga aagaacagtt tacatggaag
    20761 aatcagagga acaatatcag tttaaagaaa atacagcaac acagagaatt gaatattact
    20821 gtaagcaatg gaatataccc tattacaact tagctaatac agggaagaaa cttgctaaag
    20881 taatacataa gacaaatata ctagatatga taaaaaagga cttaaaagaa acagcaacaa
    20941 aaggtggaga cttatttaga gtaaggatgg ataataaatt aaaattattc aagcttggta
    21001 ctaatgcaaa tgtatataaa ttagatagta tattagaaga tgctaacttt acaagtagtt
    21061 ttaatgatgc agtaacaagt gtaaaagttt taggtaagag taaagacgaa aatacaaaag
    21121 cgcctataat tgggacatat aaaaaagatg ctgataagtt tggaacacta caaaagatta
    21181 aacaagatga aaagataaaa aatgctaaag aagctaagaa agcagcagaa gcaatgttca
    21241 atagtggaga ggaaacaata agtgtagatt gtgcagtaga tataaataga ataagagcag
    21301 gtgacaaagt aagtttaaaa agtaaagaat attatgttat agatgtcact catacactag
    21361 attctagacc gaaaatgaag ctgaatatag ggtctttaga atatataagg aggaaatttt
    21421 ataataatga ctgatgctag atttaatgga attgctagaa tattgaaaga aaatatgaat
    21481 aaaagtgtag caaatggcac ttttggaatg ggttgtgaac ttgcagaaat aacagcaaat
    21541 ggattaaaag taaatggcta taaagatgaa atacaggact atctagtatt agagaattta
    21601 acattaaaag aagattattt tactttttca gatgaagctt taagtggaga atatagacat
    21661 aagcataaaa tagaaactcc aaaggaattg aagccactac gtataggcga taatgtgcta
    21721 gtagctgtta tgggagctga atttgtagta attgggaggg ttgtaaatgc caaacctatt
    21781 tcctcaaagt gaaacttttg aaactgtaga attaaaaaat aatgatgaaa atgaattgga
    21841 cctaaagggt tcttttttat ttgattttga aaaaggtgaa tttgttaaaa acgcagatgg
    21901 aacactaaaa aaatgtgata aggtgcaggc gtacaaacaa tggtgtcaaa aggctatatt
    21961 aacacctaga tacaaaaaag cagcttatac aaacatttat ggaagtgaaa taaaagactt
    22021 aatagctagt aacttatctc aaagtgcaaa agagcttgaa ataactagat taataaaaga
    22081 aactattttg gttcatccat atacaaaaga agttagtaat tttatatttg tttggcttga
    22141 aaatagtaga cttgttaatt atgaatttga tgtgttaaca atagatgatg aaaatataac
    22201 catagatgga aacataaaaa ggtaggtgat tatatggaaa gagagctacc tataccatta
    22261 tttttaacag aagaggagga ctctgtacat gaaaggatgt taagtaactt tcaagatgtg
    22321 agcacactag aaggggactt tatttatgat gcaacaagac ctacagcaga gcagatagct
    22381 gaattaaaac aactaggatt acaaaataat ttaaggattg catttcctca aactagctat
    22441 ggagaatatc tagaatggtt gggagaatgc aaaggagtat ttaaaaatca accaactaaa
    22501 tcggttggta tggttacatt taatggtgct caaggaacta tcattacaaa aggaactatt
    22561 gttacaacta tagctacaga tgaaaaacag agcatagaat ttgagcttct tgaaactaaa
    22621 actataggag caaatgaaac agtagatatt aaagcagaat gtaggattgc aggaactata
    22681 ggaaatgtat ctaataacac tataactgtt ttactaggtt ctattagtgg tgttaaatca
    22741 gtttctaata aagaagattt cagaggtgga acagatatag aagatgaaga acattttaga
    22801 gaaagagttc ttgtagcaga gcaagaggac aaattaagtg gagctagttc agactatata
    22861 agatgggcta aagaagtaga tggagtggga tatgcttatg tagttcccga atggaatgga
    22921 gcagggacag taaaagtatt aatactagat aaaaacagaa aagcagcaac acaagaatta
    22981 atagataagg tccaagaata tatatatcca ttgaatatat cagaaggaga aaatagagat
    23041 gggaaagctc ctatcggtgc attagttaca gttgtgacac ctgacacatt acttattaat
    23101 gtaaaagcta gttttatatt tagtaatggc tttagtgaag aaactgtatt aaacaatcta
    23161 aaaactaaga tagataaata tttagataag attgatttag gggggacagt ctcatacaat
    23221 gctatacagg cgatagtagg ctctatgatg ctgacagatg aaggtataga agacttttct
    23281 aatcttacta taaatgatgt aaaagaaaat ataaaattgc aagaccaagt ggtcggaata
    23341 ggggaaatag ttaacgaggt ggttggatga tagcttctaa aaaaggtaaa gaaatgcttc
    23401 ttacattatc tcctatctat gaacaatcta tcataatgca aagcttatat gaagctatag
    23461 gaagcgaatt tgataatcta gaattattaa ataaagaaat agagttacaa ttattccctc
    23521 agagtgcgac atggggactt gaattttggg aaaatagagt gggtttatcc actaatatag
    23581 atgaagatat agaagctaga agaagaaagg tcattgctaa gcttcaaagt aaatatatta
    23641 tgacacctaa aagaatggct atgatactcc aatcttatac aggtgcaaac ataaaaataa
    23701 atgaaaatat atctccatat acttttggtg ttgaattaac cagtacccaa ggttttccta
    23761 aagatttaga agatttatat aagagagtaa atgttataaa accttctcat ttagctgtaa
    23821 gttataagtt agtttcttta ttgaaaagta aaacctattt tgcacaaacg gcaattatga
    23881 gcgaagaaat aactgtatat ccgtacacaa gcaaagaagt aaaagcaagt gttaaagcca
    23941 agtttgcact agctcataac atgagctcag aaacattaac agtatatcca agataggagg
    24001 tggcataaat ggctgatgaa caattttaca caatactaac aaacataggt aaagctaaga
    24061 ttgctaatgc aggaatgtta ggtaagtcag taattttaga gaagattcaa gcaggtgatg
    24121 gtggaggaaa ctactataat ccaacagaag accaaacagc attaaaaaat aaagtttggg
    24181 aagggaatat aaatgctttt gataatgatg aaaataatcc taactggatt attgcaacag
    24241 catgtatccc tggttcgata ggtggattta cagttagaga aatgggtctt atagataatg
    24301 aaggagatat gattgcagtt tgtaaaagcc ctgaaaccta caagccaaaa gttgataatg
    24361 gagctatgaa agatttgtat ttgaaattta tcatagaagt atctaatgta gagaaagtga
    24421 cattagttgt tgaccctaca gctatttttc taactaaaaa agatgaagaa aaaatattaa
    24481 caaatattaa taaactagac actaaaatag atacaaccaa aacagaatta acaagcaaca
    24541 tagaaactac taaaacagag ctaaacacta gaattgacac agaaaatgag aaacaaaata
    24601 ttaaaattga ccaattaatc gcaggtggtt caaatgtggc atctactcaa ataataacaa
    24661 ttga
//
LOCUS       DAMJVI010000049        24538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.051, whole genome shotgun sequence.
ACCESSION   DAMJVI010000049 DAMJVI010000000
VERSION     DAMJVI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.051"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(450..626)
                     /locus_tag="PC545_002095"
     CDS             complement(450..626)
                     /locus_tag="PC545_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456862.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     TCLHQVLLLVSRYN"
     gene            1201..2223
                     /locus_tag="PC545_002096"
     CDS             1201..2223
                     /locus_tag="PC545_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456863.1"
                     /translation="MVNWEIKKLAKSKSMFISLGILVLILMISIFITPVLETESYYID
                     KEKGRIEDTRSGIDIGNEKFQNKINVLKQFSNQTDTGEFSKKIELMSKKKLDNLSVNE
                     YKDISFWKVFNYRVTNSLINVGMLIIISIIISNIYVDEVVTSVKDIILSSKEKKKALN
                     SKIFVALLVPLVIYSVYLMGIFIITCMQQGVPVNGDLESFRIVDNVVALKTNLSITKY
                     VILNIGIELLMFEGWAMIAIFGSFISKSSISSISFFIFIISITKIMSVIHILPKKLLS
                     VVSNVNYYDLIFGFNKIIGNYLGDIMIFKVNIDIVNIAIGLLMAILIGAMSLCFLVIK
                     SDYISR"
     gene            2237..3124
                     /locus_tag="PC545_002097"
     CDS             2237..3124
                     /locus_tag="PC545_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2456864.1"
                     /translation="MNKLEIENLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQILYNGEDIFKMDADYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLRGKYLNSKIGELLDLVGLYDVRNKSIGKFSGGMKRRAGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKMSRDKIIILSTHIISDIESVAKETIMIKDGKLLMKGTHKD
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDINSIVLRIVSEDIPSEDNIQPVQ
                     AKFEDVYMFYFDLEDAREV"
     gene            3130..3903
                     /locus_tag="PC545_002098"
     CDS             3130..3903
                     /locus_tag="PC545_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2456865.1"
                     /translation="MMYLVKAEMIKILKSKSVWIIWLFLIFFGFFLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKKGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNESIHNLWYFGNSNLNLTVIQMYIIVILTTIFAS
                     FIFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     MVKYNVPSILIVFSIVISIILMIVLTKITYENFTSSKRF"
     gene            4015..4740
                     /locus_tag="PC545_002099"
     CDS             4015..4740
                     /locus_tag="PC545_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456866.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLNNIYTAKTKKEGLELFK
                     SINPDLIVLDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDAQNESKNLNNNEEKIISFGPYILNESRAELIKDG
                     KSIGLFAKELKILSLFAHNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLAVKED"
     gene            4744..6147
                     /locus_tag="PC545_002100"
     CDS             4744..6147
                     /locus_tag="PC545_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2456867.1"
                     /translation="MKWKITRNFIFTIVFVAISVVIINIISILYVISTNSFFKVVDSG
                     NNPEEFARSFEKDLYEKDGEFKLSKIGAEKLEKSNSWIQVLNDLGEEVYGVNVPKDTP
                     KKYTPFQMVNNYKYIETKYVNFVLEKYLNKKHLNIIVGIPSRDISRIILTYSQNNIKK
                     TLNKVIIITLVIDSVVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FEEDEIQEYTEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKINLVSLVRDIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVEIVKKD
                     NIEIIIKDNGIGISKSDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYV
                     SSEIGIGTKIIIEIKQN"
     gene            6158..6982
                     /locus_tag="PC545_002101"
     CDS             6158..6982
                     /locus_tag="PC545_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HDF2456868.1"
                     /translation="MDFFKKGISGFTIKILALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMAVEGFYHTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGAIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIILGIFTVALLFKVTNRVI
                     ALFMILVPVPFLVEGGPIWIILGIIFYLCRGKKFSLSICYVLMCIFIFTTMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(7307..7927)
                     /locus_tag="PC545_002102"
     CDS             complement(7307..7927)
                     /locus_tag="PC545_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2456869.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKETI
                     PGCISCASCFETGKCIRNDKVNKLIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            8193..8525
                     /locus_tag="PC545_002103"
     CDS             8193..8525
                     /locus_tag="PC545_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2456870.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            complement(8645..9286)
                     /gene="pcp"
                     /locus_tag="PC545_002104"
     CDS             complement(8645..9286)
                     /gene="pcp"
                     /locus_tag="PC545_002104"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDF2456871.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQEHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTII
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIR
                     GGFIHIPYTTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(9307..10239)
                     /locus_tag="PC545_002105"
     CDS             complement(9307..10239)
                     /locus_tag="PC545_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDF2456872.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVVFAKYEEPDENFGKEQADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFDNSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            complement(10261..10926)
                     /locus_tag="PC545_002106"
     CDS             complement(10261..10926)
                     /locus_tag="PC545_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDF2456873.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGVATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKASTIMAVIALVVATIQFTLYDKKLNKS
                     HKK"
     gene            complement(11496..11969)
                     /locus_tag="PC545_002107"
     CDS             complement(11496..11969)
                     /locus_tag="PC545_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2456874.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNVTIVDINNNNKIELSK
                     FDKIIIGSSIYVGSVSKKIQVLCNDNVELLNKKQVGIFLCCGFSEQADKYLKSNFPSS
                     LLESANAIGIFGSEARLEKMKFLDKLIMKAVSKGNYDSFRISQDNIDNFLINLNS"
     gene            complement(11972..12376)
                     /locus_tag="PC545_002108"
     CDS             complement(11972..12376)
                     /locus_tag="PC545_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDF2456875.1"
                     /translation="MKIRNKATNKKKKYVKISIALFFVFICIIIVIVSNLKPENLVVK
                     DIDINNVKNGIYTGSADNNLVKATVSVEVNNGKIQNINILKHDHLLGKPAEKITTSII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(12351..12980)
                     /locus_tag="PC545_002109"
     CDS             complement(12351..12980)
                     /locus_tag="PC545_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2456876.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMTNKLYNTVESNAIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKSIMYSRMNIIFASENTNNTPSNNGIKMLDKFLSIGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLIENFLDILVGGLYNENSQQGY"
     regulatory      13345..13388
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13489..14457
                     /locus_tag="PC545_002110"
     CDS             13489..14457
                     /locus_tag="PC545_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HDF2456877.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLYNHEKVSIIALAPLTNIAKALIKDKKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNIIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDIIDSVEGVI"
     gene            14459..15022
                     /locus_tag="PC545_002111"
     CDS             14459..15022
                     /locus_tag="PC545_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2456878.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDVYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQVFTDYGD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            15030..15719
                     /locus_tag="PC545_002112"
     CDS             15030..15719
                     /locus_tag="PC545_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2456879.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLKVLKNVTGKYGVLEVINSGSCFELPKDTLEKIKCIIKEKNIKKLFLESHWSYK
                     NRLKEMREYFEIPVVFKIGVETFDNDFRNNILNKNANFKTPQDVKEYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSSIKRDEELVKWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            15846..16574
                     /locus_tag="PC545_002113"
     CDS             15846..16574
                     /locus_tag="PC545_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2456880.1"
                     /translation="MMFDRVKWNKLDYNELLEYMMSIQDIKYRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISQGNWKEFLEVAEDTYYEEVRLQGMVIGNINSNFEETLYYVKRFI
                     PKIDNWSVCDGFCSDLKSMKKYKENMYDILKKYVYSKNPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKCEEETFLYIKNNNLDDFTYNKMLQKI
                     IESNRVDLEKKNIIRSMKRKTRRC"
     gene            16588..17262
                     /locus_tag="PC545_002114"
     CDS             16588..17262
                     /locus_tag="PC545_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2456881.1"
                     /translation="MNNNEDRIDKFLELQSKGFKFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYIFKDELNYNKSLDNRNLEEEKAKEYEKSKKNTISNRKKVSTKKKISNEKAL
                     SKSVEQIEFNDTGLKNAKPKQNIKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNIKP
                     KKVSNKKDVHIDDEHIDELKTTRIRIDKEIWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            17288..18124
                     /locus_tag="PC545_002115"
     CDS             17288..18124
                     /locus_tag="PC545_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456882.1"
                     /translation="MNKIVKKSISLMIILTIFIFMLTACEKDEQPDSVDIQTEDKNEI
                     KIDEGNAKVLNIGKSEIINIDEGDKVDTSTKVENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKGVEVYAYEYEAEGKIVYVNLKENTINIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEGITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            18140..18811
                     /locus_tag="PC545_002116"
     CDS             18140..18811
                     /locus_tag="PC545_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2456883.1"
                     /translation="MKVLIVEDNKILLESVVEELSKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            18801..20033
                     /locus_tag="PC545_002117"
     CDS             18801..20033
                     /locus_tag="PC545_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2456884.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDVGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKVNLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIALELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSIDINVT
                     SDVDIGTTFELSIPKKLK"
     gene            20183..20500
                     /gene="trxA"
                     /locus_tag="PC545_002118"
     CDS             20183..20500
                     /gene="trxA"
                     /locus_tag="PC545_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2456885.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            20517..21395
                     /locus_tag="PC545_002119"
     CDS             20517..21395
                     /locus_tag="PC545_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2456886.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SIDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKENHIEVDG
                     VFVIKDSTAPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYLDKLKRA"
     gene            21665..22033
                     /locus_tag="PC545_002120"
     CDS             21665..22033
                     /locus_tag="PC545_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2456887.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIKKCCKRREK"
     gene            22036..22407
                     /locus_tag="PC545_002121"
     CDS             22036..22407
                     /locus_tag="PC545_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HDF2456888.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTAVDAVVVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(22552..23007)
                     /locus_tag="PC545_002122"
     CDS             complement(22552..23007)
                     /locus_tag="PC545_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456889.1"
                     /translation="MNINTVQGDSIEVLLRQLGATRISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(23046..24311)
                     /locus_tag="PC545_002123"
     CDS             complement(23046..24311)
                     /locus_tag="PC545_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HDF2456890.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNERAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         9473 a         2718 c         3783 g         8564 t
ORIGIN      
        1 aatgcttgtt tttcttgtat tatttatata aatattgaaa tttgtctagt tatacgtttt
       61 tattgaccag ttatgcagtt gatattgaat attctaaata ttggtgttat tttataggtg
      121 gtttaaacat attaggggga ttttatatat gatttgtatg atttatagag aatgtataaa
      181 atagtaaagg tttagagcca tgtttagtgg attgcagaat atgcatatag aatgattctt
      241 gtaaattaag ttagacatag gaaatatata atttgtattg gatgattaat tttatggtgt
      301 atgcttttaa cttctataat gggaaagtaa agatgtactc ccaatacatc tttactttag
      361 cagaagttat agttacttat aaagtacaaa aaattatata tttattagag tagataaatc
      421 taaaaaggtt tttctagaaa aatttagttt taattgtatc tacttactaa taataagact
      481 tgatgtaagc aggtttcaac taccgtttct ccatattctg atataaaatc atctaactgt
      541 gtatctaaaa aattacaata atcatatgaa ctactgatgt aagcagttag tatatgtgta
      601 catttgtcta tttctccaat aaacatatta tccatccccc taaaaaattt agtaaaaata
      661 aaaagtcgca ttctattagt caattaacat aatttataaa attttttagt actagtttct
      721 ataacttaac tccttaaggt taaatattcc cagttttacc aatttagttt tattctacaa
      781 gtatataata ctagaagatg cttcattatt caacatgctt gtttgagaat agaaaatatt
      841 ctaaaattat aattgttttt aaaaataaga ttcaagataa ataaagttat atgcaactat
      901 ttaataatct ttccataata aggacagtag gttctattct taaaaaactc catctttaaa
      961 tttaaagaat aactacaatt tgaacaacaa taaaatttcc aatcaataaa aatatcaaaa
     1021 ataataaaaa taattgaaaa tgtataattt cccagaaaaa taataaatgt aactccaata
     1081 ataaacacac ctataaatta taaaataatt taagctttat ttaaggttag tattatattt
     1141 atataagatt taagttttaa tatatatgtg taattaaaat gatttaggag gagaaatata
     1201 ttggtaaatt gggaaataaa aaaattggct aaatctaaga gtatgtttat atctctaggc
     1261 atattagtgt taatactaat gattagtatc tttattacgc ctgttttaga aacagaaagt
     1321 tattatatag ataaagaaaa aggacgtata gaagatacga gaagtggtat cgacataggt
     1381 aatgagaaat ttcaaaataa gattaatgtt ttaaagcagt tttcaaatca aacagataca
     1441 ggtgaatttt ctaaaaaaat tgaattaatg tcaaagaaaa aacttgataa tctaagtgtg
     1501 aatgaatata aggatattag tttttggaaa gtatttaact atagagttac aaattcactt
     1561 ataaatgtag gaatgttaat cattatatct ataattatat ctaatattta cgtagatgaa
     1621 gtcgttactt cggtaaaaga tatcatacta tcatcaaaag agaagaaaaa agcattaaat
     1681 tctaaaatat ttgttgcttt attagttcca ttagtaattt attctgttta tttaatggga
     1741 atatttatta taacttgtat gcaacaagga gtacctgtta atggagactt agaatctttt
     1801 agaatagtag acaatgtagt tgcattgaaa actaatttaa gtataacaaa gtatgtaata
     1861 ttaaatatag gcatagagct tttgatgttt gaaggatggg ccatgatagc catatttggg
     1921 tcttttataa gtaagtcttc aatatcttca ataagttttt ttatatttat tatatctata
     1981 actaagataa tgtcggtaat ccatatatta cctaaaaaat tactttcagt tgtatcaaat
     2041 gtaaattatt atgatttgat atttggtttt aataaaataa taggaaatta tctaggtgat
     2101 attatgatat ttaaagtgaa tatagacatt gtcaatatag caataggact tttgatggca
     2161 atattgatag gtgctatgtc attatgtttt ttagtaataa aatcagatta tataagtaga
     2221 taaaagggga gttaagatga ataaattaga aatagaaaat ctttcaaaga tatatggtaa
     2281 aaaagtagca aacgacaaga ttacagtaac tctagaaaat ggagtttatg gtcttctagg
     2341 accaaatgga gcaggaaaat ctacattaat gaaacaaata acaacgttga taaaacctga
     2401 tgaaggtcag atactttata atggtgagga tatatttaaa atggatgctg attatagaaa
     2461 tattttagga tatttgccac aagaatttgg agtttataaa aattttacag caaaagaatt
     2521 tttacagtat attggagctt taaaaggttt aaggggaaaa tatttaaata gtaagatagg
     2581 agaattatta gatttagtag gtctttatga tgtgagaaat aagtctatag gaaaattttc
     2641 aggaggtatg aagaggagag ctgggatagc acaagtttta ctaaacgacc ctaagataat
     2701 agtccttgat gagccaacag caggtcttga cccacaagaa agagctagat ttaggaattt
     2761 aatagcaaaa atgtctagag ataagataat aatactttct actcatataa tttccgatat
     2821 agaatcagtt gcaaaggaaa caataatgat taaagatggc aaactcctaa tgaaaggaac
     2881 acacaaagac atattaagtc acatggaaaa taaagtatat aatgttcaag ttaaaaatga
     2941 atgtgaagtt gatgatatac aatctaaata taaggttgta agtattcaaa gtgatattaa
     3001 ttcaatagta cttagaattg tttcagagga tattccaagc gaggataata tacaaccagt
     3061 tcaagcaaaa ttcgaagatg tatacatgtt ttattttgat ttagaagatg caagggaggt
     3121 gtagtagaaa tgatgtattt agttaaagct gaaatgataa aaatattaaa atcaaagagt
     3181 gtttggataa tttggctgtt tttaatattt tttggatttt ttctgattag aaagtttgat
     3241 gttttagata cttatgcaga tatattttac aagatagaag gctcaatacc tctaataggt
     3301 ttgataatgt ttataataac ttctggaaat tacattaaag aatatgattc taatatgaca
     3361 ggtttaataa atacaactaa aaagggaaaa aaatcagtag tattatcaaa actagtagct
     3421 aatggattaa ttttatctat tattaatatt tcttttatat tgttagtagg aattagaggt
     3481 tttagttttt ttgattttaa aaatttaaat gaatcgattc ataacttatg gtactttgga
     3541 aatagtaatt taaatcttac agtaatacaa atgtatataa ttgttatatt gactactatt
     3601 tttgcttctt ttatatttgc acaaataggc ttaacacttt catctatatt taaatcggct
     3661 gtaattccat ttatactagg tggattgata atggcaattc catactttag tgtaggtttt
     3721 ataccagata aagctataaa atttatgtca gtgactccaa attggattat gatgagccaa
     3781 caaatggtta agtataatgt accaagtatt ttgatagttt tttctatagt aatctcaatt
     3841 atattaatga tagtgttaac taaaataaca tatgaaaatt ttacatcaag caaaaggttt
     3901 taactaaaaa taatagtaat tttatttata ttttagttta attaaaaata agataaatga
     3961 attttagata aaatatatgc taatatatat gtaaattatt acaggaggtt gtttatggaa
     4021 aatcgtgttt taataataga tgatgaagta gaaatattga agcttttaga aactgtccta
     4081 aaaaaagaag gccttaataa tatatatact gcaaaaacta aaaaagaagg attagaatta
     4141 tttaaaagta ttaatcctga tttgattgta ttggatatta tgttaccaga tggcgaaggt
     4201 tatgatattt gtaaagaaat aagaaaaacc tccaattctc caataatatt tttatcagct
     4261 aaaaccgaag aattagataa gttacttgga ctagcgattg gaggagatga ttatgttaca
     4321 aaacctttta gccctaagga agtggctttt agagttaaag cgcatttgag aagattaagt
     4381 tattttagtg atgctcaaaa cgaaagtaaa aatctaaata ataatgaaga aaaaattata
     4441 tcatttggcc catatatatt aaatgaaagt agggcagaac ttataaaaga tggaaaatct
     4501 ataggactgt ttgcaaagga actaaaaatc ctatctttat ttgcacataa tcaaaatcaa
     4561 attataagta aagaaaaact atgggataaa gtgtggggag aagattatgt tggatttgac
     4621 aatactataa tggttcatat aagaaaaatt agggaaaaat tagaggataa tccttcaaaa
     4681 cctgaatata ttctgacaat aaaaggtctt ggatataaat tagcagtaaa ggaagattag
     4741 aatatgaagt ggaagattac tagaaatttt atatttacaa ttgtgtttgt agctatatca
     4801 gtagtaatta taaatatcat atctatattg tatgtgattt caactaatag cttttttaaa
     4861 gttgtagatt ctggaaataa tcctgaagaa tttgcacgtt cttttgaaaa agatttatat
     4921 gaaaaagatg gagaatttaa actatctaag attggagctg aaaaattaga aaaatcaaat
     4981 tcctggatac aggttttaaa tgatttagga gaggaagttt atggagtaaa tgtgccaaag
     5041 gatactccaa aaaaatatac accctttcaa atggtgaata attacaagta tatagaaaca
     5101 aagtatgtta attttgtact agaaaaatat ttaaacaaaa aacatttaaa cataatagta
     5161 ggtataccta gtagagatat atcaagaata attttaacct attctcaaaa taacattaaa
     5221 aaaactttaa ataaagttat cattataaca ctagtaattg atagcgtggt agctttaggt
     5281 gtaggatatt tatttagtag aaaacttaca aaaccaattt caagcgtact atggagtatt
     5341 gagactatgg ctaatggaaa ttatagttta tatcttaagg acagaggtat atatgaagaa
     5401 gtttttaaaa atataaatat gttagctgat actttaagag taaatgaagt tgagagaaaa
     5461 gaaaatgaag agttgagaga ggaatggctg gcaaatataa cacatgatat aaagacacct
     5521 ctagcatcta tacaagggta tgcagagata attaatgaca aagattatga gtttgaagaa
     5581 gatgaaatac aagagtatac agaaattata tataataagt ctaagtatat aaaagattta
     5641 gttgatgatt taaatttatc tactcgatta aaaaataata ctatagttct ggacaagaaa
     5701 aaaataaatt tagtcagttt agtcagagat ataataattg atattttaaa tgataataga
     5761 tataaaaata ggaatattga atttgaaagt aatgaagatt taattgaagt atatgtagat
     5821 tcaatcttat tcagaagggc tataaccaat cttatattta attcaatagt tcataatagc
     5881 gaaggaactt tgataagtgt agaaattgta aaaaaagata atatagaaat tataataaaa
     5941 gataatggaa ttggaataag caaaagtgat ttgaaacata tttttaaaaa atattataga
     6001 ggtactaata caggtgagat gcataaaggt tcaggtcttg gtatggctat ttctaaagaa
     6061 ataatagaga ttcataaagg taaaatttat gtaagtagtg aaattggaat aggaacaaaa
     6121 attattatag agataaaaca aaattaggag atatgatatg gattttttta aaaaaggtat
     6181 aagtgggttt actattaaaa ttttagcatt aatatttatg acatttgacc atatagcagc
     6241 atttatgcca caaacaatgc aaataccaat ttggtttcat tgggtaggca gaatatcagc
     6301 accactgttt atattcatgg ctgttgaagg attttatcac actagtaaca gaaaaaaata
     6361 tataagtaga ttgtatattt ggtctgttat tatggctata ggaaatcaaa taattaataa
     6421 tgtattttca catccagaag gagctataat tataaataac attttttcaa cattattttt
     6481 aatagcgatt tatttacaag caatagagtt cataaagaaa tttagaaaag agaaagaaat
     6541 taaatatttt attataggat tattgatgat aatcattcct ataattttag gaatatttac
     6601 agtagcttta ctttttaaag tcactaacag agttattgct ttatttatga ttttggtacc
     6661 agtaccattt ttagtagaag gtggaccaat ttggattata cttgggatta ttttttattt
     6721 gtgtagaggt aaaaagttca gtttgagtat ttgttatgtt ttaatgtgta tatttatatt
     6781 tacaacaatg tcaaatggag attatagttt aaaaaattct attttacaaa attaccaatg
     6841 gatgatgatt gcttctctac ctttaatgtt actttacaat ggagaaaaag ggaagagtat
     6901 gaagtattta ttttatttat actatccaat ccatgtatat atattgtata tcttaggaat
     6961 atacttaata aatggttttt gattgtaagt gatattttta atacaaaatg aacagtatga
     7021 ggattgtttt aggtctatta aaattgttat ttagatgaga tttgtagaat atttaaattt
     7081 atgagcagaa attcagttat tgtaatttca ataacattag aatataatat tataatttat
     7141 ttagaaatat atgatgttat acatggtttg gttattaatg taataataaa acagaggttt
     7201 gatataaaat tcaatacctc tgttttagtg ctttttatcc tacatttatg cttattttat
     7261 ttataaatta gtgttttgtt ttaattaata ttagtacatt ttttatttat tttataaaat
     7321 tagtcataat tgtttcttca tattgtggtt ctttaattcc agctttttta cctgctgata
     7381 tacacttaag aagccatgcc atgttttctc ctagagttcg catagtctgt aatccttctt
     7441 catccttcat aacatcctct ggagtaaagc catgtacctg gttccaatac tgtgatgata
     7501 caataggcat attgctgatt gaaaaatatt tatttagttg gtcaaatgtt gcacttgcac
     7561 caccacgtct acatgacaca actgaagcac ctaattttgt agccatgcga cttccagcta
     7621 tgaaaaatag acggtcaaga aaagaagtta attgaccagt tgcactagaa aagtaaacag
     7681 gagaaccaat aataatacca tcaatattat ccagatcttc aatcaattta ttaactttat
     7741 catttcgaat acattttcca gtttcaaaac aggatgcgca actaatacat cctgggattg
     7801 tttctttacc taaatacagt atttctgttt taatatcgtg tttactcaat actttagtaa
     7861 tctcatttag tgctgtataa gtacatccat attggtttgg gctaccgttt atcaataaaa
     7921 ctttcattat aaatttcctc caatgaattt tatttaatag attttctaag agatattatt
     7981 ttttataaaa taggattttc atgaatatta tcaatttata tagaaatgtc taatcttaga
     8041 atcctattta aaataacttt ttttttagta aatacacctc ctattataaa attgactttc
     8101 aattattctt acactataat tttattataa attattctaa aaacaagtac gcacattttt
     8161 gttcaatact aaccaaaagg agagtgtaat atatgagtat agactgtata tcaaatacag
     8221 atttaagtga aacaggattt agttatacac tttcactgat tagtggtaaa tataagatga
     8281 taattcttta ctgtcttgta gaatttgaag ttgtaagata taatgcatta aaacgttata
     8341 taaatactat ctcctataaa actttgagtt tatcattaaa agaattagaa gcagataact
     8401 tgattattcg tactgaatat cctcaaatac ctcctaaggt agaatatagt ctatcagaaa
     8461 ggggaaaatc tctgattcca attttagatg caatgtgtga gtggggagag aggaatagac
     8521 cttaattttg tatctgtaga taccataaaa agactgtgag taaaattgtt tgaataatct
     8581 actcacagtc tatatttaaa ttaatagtta gtatttttaa tttataaaaa tactaaaaaa
     8641 tttattatga aatttcacca ccaatttctt ttacatcttc tttatatata atacaagctt
     8701 ctatagctaa ttctaagcct tttactattt cttctaaaga catgaatggt gtattttttt
     8761 tatctattac ttgtgaagtt gtatatggga tgtgtataaa tccacccctt atattaggat
     8821 atttcttgtc tatcatataa agaattccat acatcacatg attgcaaaca aatgttcctg
     8881 cagtgtttga aacactagct ggaattttat tttcattcat atgttttacc atagctttta
     8941 caggcaattt tgaaaaatat gcaggttcac catcttcttt tataatagta tctataacct
     9001 ggttaccctc attatctttt attctaaaat catctatatt tatagcaact ctctcaggtg
     9061 taatgtcaaa tctacctcca gcttgtccaa ctgatattac tatatctgga ttgtgctctt
     9121 gaatagcttt atctatagct tcaacagatt ttgtcataac tgttggtata gttatcttta
     9181 tgatttctgc accgtttata ttattattga ccctttcaac tgcttcttga gctgggttta
     9241 ttggttctcc accaaatggg tcaaatcctg ttaacaaaat tttcattatt ttacctccta
     9301 attatattaa aatcctaaaa agtacattac taatatgtgg gctataatta ataccaaagc
     9361 aactggagct tgatatttta taacaatata atcattttta cattctagca aggctgctgg
     9421 aactatatta aaattagcag ccattggagt aagtagagtt ccacaatatc ctgctgttaa
     9481 tgctaatgct ccaattatag caggatttcc accttgagac aatacaaaag gaataccaat
     9541 tcctgccgtt atgactgaaa atgcggcaaa tgcatttccc attatcatgg taaatataac
     9601 catacctaat acataagcta taacaccaaa taatctgcta tcagcaggga ctacaccaga
     9661 tatcatggtt gaaataactt caccgactcc ggcttgagta aataaagctc ccaatgcaac
     9721 taaaagttgt ggtaacatac tagcaggtcc catttgttga agtaatcttg agccatcttg
     9781 aactgatgta gcaggttttg ccttagttat tataagggct gatattaatg caattattga
     9841 acccacacct attgcaaatt gagaagaatt atcaaaatta gaaaggctca tagctactac
     9901 tactgcagca aatgctaata ttaatgatgg tataaatatt ttatttttaa gtttgtctgc
     9961 ttgttctttt ccaaaatttt catctggttc ttcatacttt gcaaatacaa cttgtttaga
    10021 aagtgttaat aaactagata atattaatat tattcctatt atattggctg gaatcaatct
    10081 accaaatatg aatggcagtg atataagtat ccaaaataat gatgtaccat atttcttatt
    10141 gttatttatg gtagtaaaag acttatatgc tataacaatc attaaaaatc ccattagtgc
    10201 gtaaaagatt tctaatgata tgtttataaa attttgcatt tttattcctc ctaaataaaa
    10261 ctatttttta tgacttttgt ttaatttttt atcatataag gtaaactgga ttgtagccac
    10321 tacaagtgct attactgcca taatagtact agcctttacg atattaagtc cagttacatt
    10381 gtatccaaat tgagtcagag tagatgctat aagtaatact ccactactac ctgcaaataa
    10441 gttttgacca taaaaatttc catagttttc tgatgcagca gataatgcct ttatggcttc
    10501 ttcatccgat tcttttagtt catcactatt acttaaacca gcagcttgtg ccataggatt
    10561 aacaagtggt cttacaaatt gaggatgtcc actcattctt attgatagag caccagctat
    10621 ttgtctagct atcatgtata tagtaagtac ttttcctgtt gtaagtcttt ttaatttttc
    10681 tattaaagaa acagctcttt gttttaaacc atatctttct aatacaccga ttactggcaa
    10741 tgttaatatg aataatgata cacctctgtt tgagacaaaa gaatctccta aaattgtaaa
    10801 aatttgatta aaatctaatc ctgcaacaat tcctgttgcc actcctgcta ataatacagt
    10861 aaataatgtg tcaattttaa gtataaatcc aattacaact atcaatatac cgattagttt
    10921 tatcataaat tgttctcctt ccatttctat atgttttaat ttaaatttat ttaatggata
    10981 aagttaaact ctttttatag tatttgcgtt aataggacta ttattataaa atttaataaa
    11041 aaatagggaa gatgttttaa agtatcaaag taaataatgg ttgtgtatag gaatttgaat
    11101 aacaaagatt atattttaag ctaatatgaa ttgattttgt aagtctatag tttaagtata
    11161 tgtcaatatt tgaaataggt tttaaattag atattatatt ataaaaagag aatgtgccaa
    11221 acatagagac aagtgtattt ttcacttttg aaatgataaa aatatatttg atttcataca
    11281 aaaagagagt gtctaatgaa ttttttagtt cattagacac tctctttttg ttatgattaa
    11341 taaaacatat ttgttatgat taataaagca tatattaagt gctagtttca ttagatttca
    11401 ctaagaaaaa taaaattttg ataaagttta aagactattt tgagcaatat tacttataaa
    11461 taaaagaatt tatcatagaa ataattagtt ttattttagc tattaagatt tattaaaaaa
    11521 ttatctatat tatcctgtga tattctaaaa ctgtcataat taccttttga aacagccttc
    11581 ataataagtt tatctaaaaa tttcattttt tctaatcgag cttcactacc aaaaattcca
    11641 atagcatttg cactttctaa taaggatgat ggaaaatttg attttaaata tttatctgct
    11701 tgttcagaaa agccacagca aaggaaaatg ccaacttgtt ttttgtttaa taactctaca
    11761 ttatcattac atagcacttg tatttttttt gaaactgaac caacatatat agaacttcca
    11821 ataataattt tatcaaattt tgataactca attttattat tattattaat atcaacaata
    11881 gtgacattat ctgatagttt attttttagt atatttgcac agtcttttgt acaaccatat
    11941 ttacttgaat atataataat agtattcata attattctcc ctttcttaaa gcattttcta
    12001 cagcttttcg tatagtatta ctgctataag ttgcagaagt tatagcatcc acatctaaag
    12061 actgttgttt aataatactt gttgtaattt tttcagcagg tttacctaaa agatggtcgt
    12121 gttttaagat attaatattt tggattttac cattattaac ttcaacagac acagtagctt
    12181 ttaccaagtt attatcagca cttccagtat aaatgccatt tttaacatta ttaatatcta
    12241 tatcttttac taccaagttt tctggcttta agttgcttac aattacaata ataatgcata
    12301 taaatacaaa gaatagagca atagatattt ttacatattt tttcttttta ttagtagcct
    12361 tgttgcgaat tttcattgta aagtcctccc actaaaatat ctaagaaatt ttctataagt
    12421 tgagaaaaat tatgtaaaga atatagttta ttttcaacaa cacataaaac gaatcctaac
    12481 aatgcactta taagcatcca tgagctttta tctatatttt ttttgaatat tccttgttca
    12541 attccaatgg aaaggaattt atcaagcatt tttataccat tattgctagg cgtattgttt
    12601 gtattttcag atgcaaatat aatattcatt ctactataca tgatagattt tgccatctct
    12661 ggttcactag aaagagtttt tataaagcta gtcataattt ctatgatttg tttatctatt
    12721 ggtagtgatg agcaattatt cataatgtca atggcattac tttctactgt gttataaagt
    12781 ttattggtca tatcgctgat aatctctgct ttgtctttgt agtaaagata aattgtagtt
    12841 ggagaatatt ctatttttgt agcaattttt cttattgata aattttcata tccatgttgg
    12901 tttataattt cgatgctagc atccataatc tttttcttca tttcttcttt ttcattttta
    12961 cgtctttctt tgatactcat ataatcacct ctttgcttaa cggtgttaag ttaatttaca
    13021 ctgttattgt ataatttaat tttaatactg tcaataaatt attaaataat ataagttaat
    13081 atatttttat ggataataaa ttaatgttat taattgtaat atttaagata ttattgtata
    13141 ataataatcg gtttaaatgc gccacatctt attaaagttg gttcataatt ctaaataaac
    13201 tttaataaga tacattaaaa tataataaat tcttttatgc tataaaattt attttatagc
    13261 tattaaatca gatgtttaaa aattttttaa cagtctatag tttttaagag tctatagtaa
    13321 gtagcctaaa aatatgattt tacatctgta cttgttcgag aactccaagt ataaaaaact
    13381 aaggaattat atagtggcag tatataatta atacccttag acacaccttt ttgaaagtgg
    13441 aaaaaagatg tgtagttctc ctcgaccaca aatcaaggag gataaaaaat ggaaaagaga
    13501 aaagtaataa ttgattgtga cccaggaatt gatgattctt tggcaattct tctggcttta
    13561 aactcaccag agctagaagt aattggaatt accacatgtt gtggaaatgt tccagcaaat
    13621 ataggtgcag aaaatgcact aaaaacactt caaatgtgtt cttcactaaa tattccagta
    13681 tatataggag aagaagcacc actaaaaaga aaacttgtaa cagctcaaga tacacatgga
    13741 gaagatggta ttggagaaaa cttttatcaa aaggttgtag gagctaaagc aaaaaatgga
    13801 gcagtggatt ttataataaa tactttatat aatcatgaaa aagtatcaat aatagcactt
    13861 gcaccactta caaatatagc taaagcactt attaaagata agaaagcatt tgaaaatctc
    13921 gatgagtttg tatctatggg aggagcattt aggattcatg gaaattgctc tccagtagca
    13981 gagtttaatt attgggtaga cccacatgga gcagattatg tttacaagaa tttatctaaa
    14041 aaaatccaca tggtaggttt agatgtaact agaaaaattg tacttactcc taatattatt
    14101 gagtttataa atagacttga taagaagatg gcaaagtata taactgaaat aactagattt
    14161 tatattgatt tccattggga acaggaggga ataattggct gtgtgataaa tgaccctcta
    14221 gcagtagcgt actttataga tagaagtata tgtaaaggat ttgaatcata tgtagaagtt
    14281 gtagaagatg gaatagctat gggtcagtct atagtggatt ctttcaattt ctataaaaaa
    14341 aatcctaatg caattgttct aaatgaagtt gatgagaaga aatttatgta catgttttta
    14401 aagaggcttt ttaaaggtta tgaagacatt atagactctg tggaaggagt gatatagtgt
    14461 gaaaagaaaa atatgtgtaa aaactattac acttatagct tttggtattt ctataaatat
    14521 aataggcgct tttatagcta tgggattaag attacctgta tacttagatt caataggaac
    14581 tataatgata gcttcacttt taggacctaa atacgctgtt gttacaggtg tgtttggaag
    14641 tttaataagt ggaattacgt ttgatgtgta ttctctctat tttgcaccag ttcaaatatc
    14701 aaccggatta cttgctggaa tagcatttaa aaagaatttt ttaaggggtt taaaaactcc
    14761 actaggagta cttttatttg ccattccaac atctataata agttcaataa tttcagcatt
    14821 tttatttgga ggtatgacct cttcaggttc atcatatata gtgcaaatct tgaaagtgtt
    14881 aggactgggg gatgttttta gtgtgtttgt aacccaagta tttactgatt atggagataa
    14941 gctgttagca gttgttttag tgaatttagg attaaatgca gttccaaaaa ctttaaaggt
    15001 atcattgact ggaggaaaat agatagaaaa tggatagata tagtgagata acaaataaaa
    15061 atcaaagaga aattgttctg cttaaaggat ttccttgtat atggggaaag tgttcttttt
    15121 gtgattatat agatgataat tctaatttag aagaagagat gaataaatta aatctaaagg
    15181 ttttaaaaaa cgtaactggg aaatatggag ttcttgaagt cataaattct ggaagctgtt
    15241 ttgaattacc taaggatact ttagaaaaaa taaagtgtat tataaaagag aaaaatataa
    15301 aaaaattatt tttagaaagt cattggtctt acaaaaatag gcttaaggaa atgagagagt
    15361 attttgaaat tcctgtggta tttaaaattg gagttgaaac ctttgacaat gattttagaa
    15421 ataatatttt aaataaaaat gcaaatttta agactccaca agatgtaaaa gaatattttg
    15481 attcaccttg tattatggtt ggaataaaag gacaaacaaa agagatgata gataaagata
    15541 tagagattat cctaaatact tttgaaaaag cgacagtgaa tgtttttata aataattctt
    15601 ctagtataaa aagagatgaa gagttagtga aatggtttgt tagtaagtat aaattcttgg
    15661 atgaaaatcc aaatatagaa gttttataca ataatacaga ttttggtgta ggagattagc
    15721 attaatttag tttaaattta aaattgaaaa gtttcttatg tttttgaaaa agcttctaat
    15781 agttagaagc tttttttgta gagttttatg ttacaataag aaaaaaggaa ttaaggagca
    15841 attaaatgat gtttgatagg gtaaaatgga ataaattgga ttataatgaa cttttagaat
    15901 atatgatgtc tatacaagat ataaaatata gagattttaa taaaaagctt atacctggaa
    15961 cagaaaatat aataggtatt agagttccta atttaagaaa gttatcaaaa gaaataagcc
    16021 aagggaattg gaaggaattt ttagaagtag cagaggatac atactatgaa gaagttaggc
    16081 tacaaggtat ggtaattggc aatataaata gtaattttga agaaacttta tactatgtaa
    16141 aaagatttat acctaaaatc gataattggt cagtttgcga tggattttgt tctgatttaa
    16201 agtctatgaa aaaatataaa gaaaatatgt atgatatact taaaaaatat gtttattcaa
    16261 agaatccatg ggaaattaga tttgcattag ttatgttttt aatatattat gttgatgata
    16321 aacatattaa agaaatattt gaatactgta ataatataca aagtgaagaa tattatgtta
    16381 aaatgggtat ggcatggttg ctatcaattt gttttattaa atgtgaagag gaaacttttc
    16441 tctatataaa aaataataat ttagatgatt tcacttataa taaaatgcta caaaaaataa
    16501 ttgaatcaaa tagagtagat ttagaaaaga agaatataat aagaagtatg aaaagaaaga
    16561 caagaagatg ttgacggagg aatattaatg aataacaatg aagacagaat tgacaaattt
    16621 ttagaacttc aaagcaaagg atttaaattt gcagagatag ctcaaaatct aaagattagt
    16681 caaagtactt taagaacttt tttaaataaa aaaggatata agtcagaaaa aggtaaatac
    16741 atatttaaag acgaattaaa ttataataaa agtttggata atagaaactt ggaagaagag
    16801 aaagctaagg agtatgaaaa aagtaaaaag aatactataa gtaatagaaa aaaagtaagc
    16861 accaaaaaaa agatttctaa tgaaaaggct ttaagtaaaa gtgttgaaca aattgagttt
    16921 aatgatacag gtttaaaaaa tgcaaagcca aaacaaaata ttaagcctaa gaaagataga
    16981 aaaataaata taactcaaga agatttagat aagctttgtg aagtatatga ttggtattta
    17041 gaagtaaaag attacaatat aaagcctaaa aaagtatcta ataaaaaaga tgtacatata
    17101 gatgatgaac acatagatga attaaaaaca acaagaatac gtatagataa agaaatatgg
    17161 aatgattttg aaagattatg tagtaattcg aattttgaaa aacatcaaat acttacacag
    17221 gcattgaaag aatttatgaa aaagtataaa aatctactat aaaatactac tatatgaggt
    17281 gcttaatatg aataaaatag taaaaaaatc aatttctcta atgattatat taacaatatt
    17341 tatttttatg ttgacagcat gtgaaaaaga tgaacagcct gattcagttg atattcaaac
    17401 agaagataaa aatgagataa aaatagatga gggaaatgca aaagtattga atataggaaa
    17461 atcagaaatt ataaatatag atgaaggtga taaagtagat acatcaacaa aggtagaaaa
    17521 taattcaaca tttaatatat ccaatgttga attgatatat aatgaatatg atgagaataa
    17581 aaaaattata tcttcggata gtaaggcttt gttggacatg actttaatgc ctgggaaagt
    17641 tgcttacata gaatgtgggc ataaaacttt tgtaaagggt gtagaagttt atgcatatga
    17701 atatgaggca gagggtaaga ttgtgtatgt taatttaaaa gaaaatacta ttaatataag
    17761 aaataataat attaagctag aaaatagtag tcagtatgag gtgctatcta catcagaact
    17821 taaaaaagta aatgaatcaa atgagggaat tacgtatcaa gtaaaagtaa aaaattcatc
    17881 atcaaaagat ttaggaaata ttattcttaa gacagcagaa gttaatgaaa atggagaata
    17941 tttaacagta aatcgtgtgc catcatataa gattttaaag ccttcagaag agacagatat
    18001 agatatattg tgttcaacaa aggcaaaaag tgttgaaata gtgggatata cgtatgatga
    18061 tataaaagaa aaagccaatg tggatattga cttaaaatct cataaagtta agatagacaa
    18121 ataaaggtag gggatactta tgaaggtttt aatagttgaa gataataaga tacttttaga
    18181 aagtgtagta gaagaactta gcaagcattt tgaaactgaa aagtgtgaag atggagaaga
    18241 agctttatac ttagtaaatc aaaatatata tgatttagtc attttagact taatgcttcc
    18301 taatataaat ggatttgata tcctaaagaa aatgagagtt aataatatag atacacctgt
    18361 acttatattg acagcaaaag aaacattaga tgacaaagta gaagcattta ctataggagc
    18421 taatgattat ttgacaaaac ctttttatat ggaagagtta gtagctaggg tatacgctat
    18481 attaaggact aatggaaaga taaaagaaag aaacggatta gaatttaaat ctttatattt
    18541 agatacacta gaaaaaagag tgtatattga aaaagaagaa ataaaacttc aaaacaaaca
    18601 gtttaacttg cttgagtatt ttgtattaaa taaaggttca attttattaa aagagcagat
    18661 ttatgataga atatggggaa ttgattcaga tgcgactatt gaaatagtgg aagtatatgt
    18721 aagtaatcta agaaaaaaac taagtaaata tggatatgat aaatatatta aaactaaacg
    18781 taaggtagga tatatattcg atgataaata agaatgtatt taccagtact aaaaatcatt
    18841 taataaaaat gtatattatt gtagtaggta gttttttaat aatcttttct atatttatat
    18901 actcatattt taggggactt acatatagtg gaattgatag tgagataaat gatgaacttg
    18961 aatatatagt ctctcaattt aaaagaactt catttttgaa cccaataaga ctgaaagatc
    19021 ctaaagatat ggtgtatgtg tatgaagatg gaaggataag ttactatact caaaatgaat
    19081 attttgatga gttacttcca gatagaagac tagataaaaa aaattcgttt tttaaataca
    19141 cagaaaatgg atatactttt agagaattaa atgtagatgt aggtagatac caaatacaga
    19201 taattagaaa tatagattct gagatgaatt ctttaagaca gcttacatct gttcttataa
    19261 ttggaatatt aatttctgta ataattactt actttgtggc tgtatatctc actagaaaag
    19321 ctttaattcc aatagaaaca gcatggaaaa atcaagctaa gtttatacaa gatgcttctc
    19381 atgaattaag aacaccaata acaatagttt catcaaaatt agaaagtatg ttaaaatccc
    19441 cagaaagtac agtaaatgat gaagttgaaa caatagctac tgcaatgaag gaaactagaa
    19501 gacttaaaaa aatgataact gatttattgt ctcttactaa agaggactct attgttaaag
    19561 ttaatttaga agaaatagat ttagaaaagt tattggaaga aatatcagaa gactatattg
    19621 acattgctga gtttcaggaa aaaagattcg tgtttaattc taaacttaag aataaagtta
    19681 taataacaga taaaaataaa ttaagacagc ttatattaat tttcattgat aatgccttta
    19741 agtatacaaa attgggtgat gagatagcat tagaattgaa agaagatata gaggatgagg
    19801 taactttact tataagtgac actggtatag gcataaaaaa agaagagata cctcttatat
    19861 ttgatagatt ttttagaagt gaaaatgtaa gaaataaaga tttagaaggt tctggaatag
    19921 gactttcaat agctcgcatg attagtttaa acttatctat tgatataaat gtaacttctg
    19981 atgtagatat tggaactaca tttgaattgt caatacccaa aaagttaaaa taataaaaat
    20041 ataaaaaatt gtttttagaa atgaagaatg ggtatactaa ttagtataaa atataaaaag
    20101 accttataaa agtaaattta tatttataaa tttcatagta taattatata gggataataa
    20161 agtttaatag gaggatttaa aaatggcaaa agttataaat acatctgaat ttagaagtag
    20221 tgttgaaggt agtaagggag tagttgtagt tgatttcttt gcaacttggt gtggaccttg
    20281 taacatgttg ggacctgtat ttgcagaatt aggtgaagag atgaaggata aagctagatt
    20341 tgtaaaagtt gatatagatg agagcttaga aatagctcaa caattcaatg tatctacagt
    20401 accaactatg attatattta aagatggaaa accagtagaa actttaattg gattcatgcc
    20461 aaagaacaaa attgaaatgc aagtaaaatc atatttataa aaaatgggtg agtattatga
    20521 gatatgatat agctataatc ggcagtggtc cagctggatt gtctgctgct ataaatgcaa
    20581 aaataagaaa taaaacaatt atcatgtttg gtaatgataa tttaagtaat aagcttgtta
    20641 aagctccttc aatagataat tatttaggtt tttatgatat aagtggtgat gaattaaaag
    20701 acaagtttaa atcacatata gatagtatgg atatttcaat tgaaaataaa agaataaata
    20761 atatttatgc tatgggagaa tactttacaa taatgtctgg aaatgatatg tatgaagcta
    20821 ctactgtaat ccttgcaact ggggtagagt atactagacc aatcaagggt gaagaagaat
    20881 ttctaggaag aggtgtgggc tactgtgcta catgtgatgc accactttat agaaataaaa
    20941 aggtagcagt tataggatat aatgaagaat ctaaagaaga agcaaatttt ttaagtgaac
    21001 tcacttcaaa gacttatttt atacctatgt acaaaaaaga taatcttatg agaagttcag
    21061 ataacctaga tgattctatt gaagttatac atgacagacc agtacagata gatggagata
    21121 aacttgtaaa caaagtatct tttaaagaaa accacattga agttgatgga gtattcgtta
    21181 taaaagatag tacagctcca agtgcattgg ttcctggaat agagattgat ggcattcata
    21241 ttaaagttga caataatatg aagactagta tagatggatg ctttgctgct ggagattgtg
    21301 ttggaaaacc atattcttat ataaaagcag ctggtcaagg gcaaatagca gctttaaatg
    21361 ctgtttacta tttagataaa ttaaagaggg cttaatattt atattgatta actttttaaa
    21421 gatatttata aaaaactgtc ttgaaataat tttattattt caagacagtt ttttattttt
    21481 aagttattaa taaaagtatt ttaaaatcta tatgtaatta cttttattaa taaaactcaa
    21541 agttatatta gtaaaaattg gaattattta taatatactc caaaatttaa aaataatggt
    21601 tgaaaaatct aattaagagt actataattt aattaagtaa ttacttaatc aaaacgaggt
    21661 acttatggaa aacttggtta aaatatttaa agcattgagt gatgaaacta gacttaacat
    21721 acttatatta gtatccaaga gaaatatatg tcaaaagggg atttctaaat atctagggat
    21781 ttcagattct gctgtttcac aacatataaa aatattaaaa gatgttggga taataacagg
    21841 atataaagaa ggatactatg ttttatatca tattaataaa gaatcattta atatatgtgt
    21901 agattttata aattctatgt taagtaattc tagtgaaagc tttatagatg tatttgatgt
    21961 aaatacaata catctaggat gcagtaaaga atgtaaatct ataaaaaaat gttgtaagag
    22021 aagggagaaa taataatgaa agtatgtata ccagtagagg agaataaagg attggatagt
    22081 aaaccatatg gacattttgg ttctgctcca atttttgttg tgtgtgattt agaaagtgga
    22141 gaagttaaat ctttagataa tggagattta gatcatgaac atggaaaatg tcaaccatta
    22201 aaagcattgt cagggacagc tgtagatgca gtagttgttg gaggaatagg acaaggagct
    22261 ataattaaat taaatggaat gggaataaaa gtttacagag cagaaggtga tactataagt
    22321 gctaatttag acttacttaa aaatggaaaa ttagtagaat ttccatcaga ccatacttgc
    22381 tcacatgacg gatgtggaca tcattaaaaa aaaacagctg atgaaatata tacttcatca
    22441 gctatttttt cttttaataa atacgatttt attcttttaa aaccaaaatt tagattttta
    22501 tattttttgt agtatcctta ataatatttc aagtttacaa gtttactact tttagatttt
    22561 ttttgtatgt ttttctatat agtcaatatc atttaatatg tcattgagtt ctttattaag
    22621 tgtttttaaa tcatcaccat ctacattgta gtttaaaaat agttcctcta cattatcaat
    22681 gatagaattg acttttctag taacttcaac aaatgtctta aagttactac tatttttagc
    22741 atttagaaat ttttttaaat cctttgatat aaatgatact atttcatatt catcattaaa
    22801 tagacctcta ccaataggat atggttcaat tctctgtaaa aaatattggt ctttagcatt
    22861 aatattataa gtataagata tttcccaacc gtctcctaaa tcaaacttga taaaatataa
    22921 tgtactactt acttttgata ttcttgtggc accaagctgt cttaaaagaa cttcaatcga
    22981 atcaccttga acagtattaa tattcatata tacacatccc ctttagtatt attatactat
    23041 attttttaaa attttataat taaataaaca gtagatataa taatagatat aatcataagt
    23101 ggaaaaccta ttttgaaata ctccttaaaa gagataggat gaccgtgttt attacctata
    23161 cctgctagta caacattagc agatgctcct attagtgttc catttcctcc aagacaagct
    23221 ccaagagatg ttgcccacca tagtggcatt acatcaatac cttcagcttg catagtcaaa
    23281 ataagaggaa ttagtgttgc aacaaatgga atattatcta agaaagaaga gactatagct
    23341 gacaaccata atatcaacaa cattgtaaat acaagatgtc cttctgtaag tccaatcaat
    23401 gcttgagcca atttgtttat tatcccaact tctactaatc caccaactac tacaaataat
    23461 cccataaaga ataaaattgt agaccattca atacctgcca taatttcatc tacatcttgt
    23521 tttcctatta aaagcattat agatgctcct gtcagtgcta caacagaaga atctatgtgg
    23581 attgtactgt gaaacataaa accaaaaagt atgaatgcta ttaatataag gctttttata
    23641 agtagaggtt tatctttaac agattttttt tcatctagtt ttaatattgc atttttagct
    23701 ctttcattca caacaagttc ttttccataa ataaatctaa agcatattat agttatagca
    23761 agtattacta taacagcagg acctaaattt attacaaaat ccataaaact aagattggca
    23821 gcactaccaa tcataatatt tggagggtct cctataagag tagctgttcc tcctatattt
    23881 gaagctaaaa tctgtgttat taaaaatgga actggattaa gtcctaatat ttgtgtaata
    23941 actatagtca ttggtccaat aagtaagacg gttgtaacat tatctaatac agctgataaa
    24001 atagctgtta taatagcaaa gcaaatcatt atcttccatg ggtcaccctt agctttcttt
    24061 gcagtccaga tagctatata ttcaaataac cccgagtttt tgacaacagc tacaaatagc
    24121 atcataccaa ctaatacacc aatagtattg aaatctatat ggcttaatcc ttcatcaagt
    24181 gtaagtatat tgaatattat cattaatagt gcaccaccaa cagctgctac ggatctgttt
    24241 accttttctg taataatagc tgccataact attaaaaata tacatatagc tattatttgt
    24301 tgaaattcca taatataagt acctccattt gtattatttt gtgcctaatg ctagtactat
    24361 atatagtatt acttttttat tgttttttca atggatgtat gaaagtatat tatttaacaa
    24421 agtttttgaa aaattgacag aatttaaaga aaatttttgt tatttaaata aattttaaaa
    24481 ttattccatt gataataata ttaaacgaca aacgaaaaaa tatacccttt aaactaga
//
LOCUS       DAMJVI010000050        24524 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.052, whole genome shotgun sequence.
ACCESSION   DAMJVI010000050 DAMJVI010000000
VERSION     DAMJVI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24524
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.052"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            243..620
                     /locus_tag="PC545_002124"
     CDS             243..620
                     /locus_tag="PC545_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2456891.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDVTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIRVKHKDYLSFETKDFLSNIHDDSLRSLISALH
                     DDENIGKDKLDFLESYFDNLKDE"
     gene            613..1761
                     /locus_tag="PC545_002125"
     CDS             613..1761
                     /locus_tag="PC545_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HDF2456892.1"
                     /translation="MNNYMLYVFENLIKTTIVCSLGICLLLFLKRYLFKKFSKRFNYY
                     IWLIIVFRMLLFLFNYTIVYEVKEPKENALGNNITQIDISTDNNLMLYVAYLWLFVTI
                     VIAVYTFIKYTRFKNLVVDVSYDIEDNDINCIYKNLLKELNIKKKIELRGSDELISPA
                     GMGLFKSYIFLPDYPYSKDELTWILKHELMHFKNKDILIKFLVLSVRIIYWFNPLVYV
                     MSNKVNLDCELCCDESVLVECSLKDKKEYALALIKSIKFSKNYNSKILTTEFDKTNLE
                     KRLESIVKKKGKSRILIAVLVFMTSITSFVEVDAQVRIDEGNNGMNNNINEFLLNKTT
                     DKIINIYYKDAPNEIRKFYEDKCELEGKIPRDYDIIEMDMKDYNQLIK"
     gene            1778..2380
                     /locus_tag="PC545_002126"
     CDS             1778..2380
                     /locus_tag="PC545_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HDF2456893.1"
                     /translation="MLTKLRKIVAIMCCLLLTFTSVAFADTSKEKGGSKETVPEKNLD
                     SFTLSGYIYTYENAPQEIRDEHKQNCEEINIDPKPDDEIFVPESANLMNEDNSKGVYA
                     SYTVSYNIGAKTITIMSNEYLTISTTKVIRKGNSGKEVKAAQIMLTLLGYNVGIDSSF
                     GSKTYNAVVSFQKKYGLSADGIIGPATWDKLGRLTDPTLS"
     gene            complement(2473..3495)
                     /locus_tag="PC545_002127"
     CDS             complement(2473..3495)
                     /locus_tag="PC545_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2456894.1"
                     /translation="MDYNDLLKSARENFNGTCKVCKICNGLACAGDVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTILNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNRGNGIVFIKPWNNSKIIDKI
                     RLSEEAGAFAVGVDLDACGLINNQFQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMAVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKSVKGKITILVDGGVRTGVDV
                     VKMLGLGADAVLMGRPFVTASFGGGLDGVEFFIEKVRNELCETMILTGCQNVKDIDGR
                     VIWNLL"
     gene            complement(3824..4006)
                     /locus_tag="PC545_002128"
     CDS             complement(3824..4006)
                     /locus_tag="PC545_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456895.1"
                     /translation="MYKDKSDECIHLMTAYIDSISGYYSFIDTQLEDFMMKYGENIVD
                     SNLHSIMMLLCKWGLS"
     gene            4319..4738
                     /locus_tag="PC545_002129"
     CDS             4319..4738
                     /locus_tag="PC545_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HDF2456896.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYIFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIVQIENCIV"
     gene            4869..6710
                     /locus_tag="PC545_002130"
     CDS             4869..6710
                     /locus_tag="PC545_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456897.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLTNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGFSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     LSLKNEDVNKANLYMDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIET
                     LYIKIKEINDFNCKKIYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSLC
                     NIKIGECECSFENYECSMEYFNEVISNKKYRNVNIIQKYRASNNITKILIKTRNYPVA
                     INNLAKSEVYLEKIKNHSLKEVEKFDLFISLAMYYAKSNEKSFEQSICYLDRAKNILN
                     MIKNIEEYIIYDLEIIYHQAKIYYIFGYYDNALNTNKKLLEKSKNAKNYNYVKLAYKS
                     IYLCFEKIQNYEMSMKYFKMYYELKMMYIKARNRNYIDSLNYRHEYIEKEINNMRKVK
                     LDLDKKKYVDHLTSAYNRTFLNDFLEKQEVSEYCTAFMIDVDYFKEYNDTYGHYNGDI
                     ALKNITIIIKKYLKKDMLMIRYGGEEFLILSVCKDYKKSKIFGRKLCRIVKKFLNNNL
                     TVSIGIDTCKNSSIDIKEIIENADKALYKAKESGRNRCLHYHDFKNF"
     gene            6737..8623
                     /locus_tag="PC545_002131"
     CDS             6737..8623
                     /locus_tag="PC545_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456898.1"
                     /translation="MKRSLIEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNT
                     KLEDNSRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDSDIISKIYIYLT
                     LSAISIRKYDKVNNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIALVE
                     EVSFFKTLKNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLKLAQENNLYSIEAFC
                     KCIISLCYLESGNNVEAIKYINNVLDVAHSNNSISVTERVSIKIDLIWAYLKEDMNKE
                     AEVILLKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYK
                     SFGDVFIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYG
                     LIANGYELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLV
                     DDNKKLENDILKDGLTKLYNRRYLEQQLDLFNETRDKNMVAILVDIDCFKSYNDNYGH
                     LAGDKVIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESIGVEEIDNIELYIKRV
                     FKLLEFEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEILIEDADKNLYKSKKAGRNQ
                     YIII"
     gene            8631..10631
                     /locus_tag="PC545_002132"
     CDS             8631..10631
                     /locus_tag="PC545_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2456899.1"
                     /translation="MILLRKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEIVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEDVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEMDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRDFKTKKLLPNSEVDKFNSETTASVEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            10893..12152
                     /locus_tag="PC545_002133"
     CDS             10893..12152
                     /locus_tag="PC545_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2456900.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKAFGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINN"
     gene            12213..14000
                     /gene="iorA"
                     /locus_tag="PC545_002134"
     CDS             12213..14000
                     /gene="iorA"
                     /locus_tag="PC545_002134"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HDF2456901.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKEYVKNAKKMVTVPANAQIRRGVVEERMEILKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTNDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDDNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            14002..14577
                     /locus_tag="PC545_002135"
     CDS             14002..14577
                     /locus_tag="PC545_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HDF2456902.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELSRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            14630..15700
                     /gene="buk"
                     /locus_tag="PC545_002136"
     CDS             14630..15700
                     /gene="buk"
                     /locus_tag="PC545_002136"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HDF2456903.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDKGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGQYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGKVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            15918..16619
                     /locus_tag="PC545_002137"
     CDS             15918..16619
                     /locus_tag="PC545_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456904.1"
                     /translation="MGKISLKSMIRLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVVIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVFMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSN
                     EHKCKSSIVIKNEQN"
     gene            16679..17185
                     /locus_tag="PC545_002138"
     CDS             16679..17185
                     /locus_tag="PC545_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDF2456905.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEEGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEDSLEGAIREAKEEIGIDITKDEMKVFRSMIH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLYE
                     IDNNKLNI"
     gene            17360..18382
                     /locus_tag="PC545_002139"
     CDS             17360..18382
                     /locus_tag="PC545_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2456906.1"
                     /translation="MEDNKGLARDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSSLNIPDSLIIIVLYALFAILLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKNFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMGFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAVETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(18529..18837)
                     /locus_tag="PC545_002140"
     CDS             complement(18529..18837)
                     /locus_tag="PC545_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HDF2456907.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINERNASCETFEMK
                     "
     gene            complement(18897..19325)
                     /locus_tag="PC545_002141"
     CDS             complement(18897..19325)
                     /locus_tag="PC545_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456908.1"
                     /translation="MNNKLHELEKNLPEISCSFCTHLSFKGPSLDYRYDIRCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFKCSICGSTENVDVYHVNKNLGRETSKDVVVKCDKCNCK"
     gene            complement(19548..21005)
                     /locus_tag="PC545_002142"
     CDS             complement(19548..21005)
                     /locus_tag="PC545_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2456909.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGLIFLYYLCATLFPIDKIIGKVYPIFGAILLLSAAGVGIGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELVGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIIKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNI
                     NISYVLAAIFTVLYAILTVRSGRKLKSKADTKLAD"
     gene            21423..22337
                     /gene="nadA"
                     /locus_tag="PC545_002143"
     CDS             21423..22337
                     /gene="nadA"
                     /locus_tag="PC545_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDF2456910.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTVLMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYSTLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            22350..23651
                     /locus_tag="PC545_002144"
     CDS             22350..23651
                     /locus_tag="PC545_002144"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDF2456911.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMKAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFIDVPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            23632..24468
                     /gene="nadC"
                     /locus_tag="PC545_002145"
     CDS             23632..24468
                     /gene="nadC"
                     /locus_tag="PC545_002145"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDF2456912.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDENSKSTVDLICKQD
                     GIIAGLGVFKRVFEILGDVDVKLYKNDGDRVKNREKIAVLTGSTRNLLVGERVALNYL
                     QRMSGIATITSRYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFNLSDGI
                     LLKDNHIGAAGSVKKAVELARKNTSFVRKIEVEVETLDMVKEAIEANADIIMLDNMSL
                     EMAKQAVDIINGRAIVEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDISMKNLR
                     NI"
BASE COUNT         9689 a         2607 c         4185 g         8043 t
ORIGIN      
        1 gaaaatatag gaatttttat taaatagttt taatgtgtaa ataacattct acactattgt
       61 aattaaatat gtaaaaagta aaatatttaa atagatatat tttaaaatct aacttttaat
      121 attgtaaaaa aattagaaat ttaacaaaaa gtacaaaaat tcacattttt ttcataaagt
      181 gtttgactaa aaaattcaca tgttgtagaa ttaaggtagc aggagttttg agggaggtgt
      241 aaataatgat aagaaaaata ccacaagcag aactaaaagt aatgaaattc atttggaaag
      301 tagatgttac agtgacatca aaagatgtta ttgaagctat ggaacaaaaa tatggctgga
      361 aacaaaccac cacactgaca cttttatcta ggctggtaaa aagaggattc ttagatgctc
      421 aaaaaataga cagatataca cattacacaa taagagtaaa acacaaagat tatttaagtt
      481 ttgagacaaa ggatttccta agtaatatac atgatgactc tttaagaagc ttaatttcag
      541 cattacatga tgatgaaaat ataggtaaag ataagctaga ttttctagaa agttattttg
      601 ataatttaaa ggatgaataa ttatatgcta tatgtttttg aaaatcttat aaagacaact
      661 attgtatgta gtcttggtat ttgtctatta ttatttttga aaagatattt atttaagaag
      721 tttagtaaga gattcaatta ttatatttgg ttgataatag tctttagaat gttacttttt
      781 ctatttaatt atacaatagt gtatgaagta aaagaaccca aagaaaatgc attaggaaat
      841 aatattacac aaattgatat ttctacagat aataatctta tgctgtatgt agcttattta
      901 tggttatttg taactatagt cattgcagtt tatacattta taaagtatac aagatttaaa
      961 aatctagttg ttgatgtatc ttatgatatt gaagataatg atataaactg tatctacaaa
     1021 aacttgttaa aagaattaaa tataaaaaag aaaattgaat taagaggttc tgatgaattg
     1081 ataagtccag caggtatggg attatttaaa tcttacatat tcttaccaga ctatccttat
     1141 agtaaagatg aactaacttg gattttgaaa catgaattaa tgcattttaa aaataaagat
     1201 attttaataa agtttttagt tttgtctgtt agaataatat attggtttaa tccacttgta
     1261 tatgtaatga gcaataaagt aaatctagat tgtgaattat gttgtgatga aagtgttttg
     1321 gtagaatgtt ctttaaaaga taaaaaagaa tatgcactag cacttataaa gtcaattaag
     1381 tttagtaaaa attataatag taagattttg acaaccgagt ttgataagac aaatttggaa
     1441 aagagacttg agagtattgt taaaaagaaa ggcaaaagta gaatactaat tgctgtttta
     1501 gtatttatga cttctataac atcttttgtt gaagttgatg ctcaagttag aattgatgag
     1561 ggtaataatg gaatgaataa caatattaat gaatttttat tgaataagac gactgataaa
     1621 attataaata tttattataa agatgcacca aatgaaatta ggaaatttta tgaagataaa
     1681 tgcgaattag aaggtaaaat acctagagac tatgacataa tagaaatgga tatgaaagat
     1741 tataatcaat taataaaata attataagga ggattttatg ttaacaaaat taagaaaaat
     1801 agtagctatt atgtgttgct tattgcttac ttttacaagt gtggcatttg ctgacacaag
     1861 taaagagaaa ggaggaagta aagagactgt tccagagaaa aacctagatt cttttactct
     1921 ttctgggtat atatatacat atgaaaatgc gccacaagaa ataagagatg aacataaaca
     1981 aaattgtgaa gagataaata tagaccctaa gccagatgat gagatatttg ttccagaatc
     2041 agctaatcta atgaatgagg acaatagtaa aggtgtttat gcaagttata ctgtttctta
     2101 taatataggt gctaaaacta taacaattat gagtaatgag tatttgacta taagtacaac
     2161 aaaagttata cgaaagggta atagcggaaa agaagtaaaa gctgcacaaa tcatgttaac
     2221 tttacttggt tataatgtag gtatagatag ttcttttgga agtaagacat ataatgcagt
     2281 tgtaagtttc caaaaaaaat acggtttgtc tgcagatgga ataattggtc cagctacttg
     2341 ggataaattg ggtagactaa ctgaccctac tctttcatag gattaaaatc taaaaatata
     2401 ttaaatagtg tctcaatatg atttttcaat ttaaatgaga cactatttaa atataaatgt
     2461 tataaaacta ttttacaata aattccatat aactctacca tctatatcct taacattttg
     2521 acaaccagtc aaaatcatag tctcacataa ctcattccta accttctcta taaaaaactc
     2581 cacaccatca agaccaccgc caaaagaagc agtaacaaaa ggtcttccca tcaaaacagc
     2641 atcagcaccc aatccaagca tcttaactac atcaacacca gtcctaactc caccatcaac
     2701 aagtattgtt atctttccct ttacagactt agctatatct ggaagtacct cacaagtacc
     2761 aggagtataa tcaagtactc ttccaccatg atttgaaaca ataatggcac tagcaccaga
     2821 ctctacagcc atcaaagcat catctacagt cataattcct tttataataa aaggtaatct
     2881 agtggattca accaattctc taatctcatc aattgtttta ggagaaaaag gattttcttg
     2941 aaattgatta ttaataagtc cacaagcatc aaggtctaca ccaactgcaa aagcacctgc
     3001 ttcttcagac aatctaattt tatctattat ctttgaatta ttccaaggct ttataaatac
     3061 aattccattt cccctattat ccttcaatac atctaaatta tccagtaaaa aagtatctac
     3121 attagtatcg ccaaccatag cataaattcc agaattagaa caacctctaa caacaggctc
     3181 tatgtattct ttttcggaaa ctttaccacc catgttcaat atagtacctg atacaggtgc
     3241 agcgaatata ggagaactca tttttctacc aaataattct atggatgtat ctggctttga
     3301 aacattatgt atgactctca tatttatctt tattttttct agactttttc tattttcaat
     3361 gaaagacgaa ccacttcctt taccacccat accaggaaca tctccagcac aagctaatcc
     3421 attacaaatt ttacatactt tacatgttcc attaaagttc tctctagctg attttagtaa
     3481 gtcattgtaa tccatacaaa atctctcctt tttataatat taaaattgag tattactata
     3541 ttgaaaatat cttaattgta aatataggga tgttattatg atgagctaaa ctagataata
     3601 catattagat attttttatt gttgtagata gttaaattat atcatattag atagtcaaat
     3661 tttgaaaaaa actaacttat ttaagtatac attttgaaaa aattaaaaaa gctatatata
     3721 gatttttaaa gaaatctata tatagctttt aagtttatgt caatcttttt tgttaatgaa
     3781 taaataatat agaatacaat atacaaaaaa gtttcttgaa atgctaagat aatccccatt
     3841 tacaaagcaa catcattata ctgtgtaaat ttgaatcaac aatgttttca ccatatttca
     3901 tcataaaatc ctctaattgt gtgtcaataa atgaataata tccacttata ctatcgatgt
     3961 atgcagtcat taagtgtata cattcatcac ttttatcttt atacatatat atctccccct
     4021 atatacaata tataaattta gataccaaat cgtatcatat taaatagtta agtgtgtaac
     4081 atctatgcag ttatatattt atatcattat attaataact aaagtgagtt ttgacaatag
     4141 attttgggaa actggatgtt caaagtgcaa acttggattt taaaatcatt aattaagata
     4201 gcgcatagga atatatgtat acataactta attataaaga ttataaacaa ttacttttat
     4261 taactttatt taaatggtag aataacttgg agattaataa ttataaggag gatataatat
     4321 gagtttatat aagtttatat atgatgataa ggaatattta ttaaaagagg agaactgtag
     4381 tgctttaata aatgatgaag aaaatccagt tcaaggagta agtatttcaa aaataattga
     4441 tatacttaat gaagcagaag aagttgattt tgatgtagaa tattatcaag aagcatgtcc
     4501 attatgttta gaaggagtaa aagaaaagaa aaaattcttt ccttttttag aatatcactt
     4561 ttatattttc agtaaagatg gagaatatgt aataagcaat atatctgttg attataaagg
     4621 actatctttt aataaactat caagggcaaa taaagttgat aatagttata ttgtaagtgt
     4681 gataatttgc gaaaattgtc aggattatat agttcagata gaaaattgta tagtttagtg
     4741 taaagtatgt cttaatttaa gagaaatttc taaattgaga catcaacttt tttcaaaaat
     4801 agtatggaaa attactaaat ttatgagata atgtaagtcg gaggtgagtt acaatatata
     4861 gtaaatattt ggaagagata gatgatacaa ttttagattc aatgtttaaa ctaaccttga
     4921 atgaaagatt attgactaat aacgatttta ttgaaaataa aattgatgta gtaaagaatt
     4981 taattgattg ctataaaact gaacaaaata gtttaatgtt attaaaatca aattttttgc
     5041 tagctttatt atatggtatt caagggttta gtgaaaaaat gaaagagtat attttgattt
     5101 cctgtaaata tattgataaa tgcttaccca aagattataa atttttagca cgattttatt
     5161 cacaaatagc tattttaagt ttaaaaaatg aagatgtaaa taaggcaaat ttatatatgg
     5221 ataaatttaa tcttatttgt gatgaaaata attttctaat tgaagaaatt atttttaaat
     5281 cacaacttat ttacattaag gcaagtaagt gtatagaatc ttctgtaata attaaagaga
     5341 tagaaacgtt gtatataaag ataaaagaaa ttaacgattt taattgtaaa aaaatatatt
     5401 tttttattat tggtaaaata tattttttgt ttttggatga tgctctaatt gctaaggtaa
     5461 attttttaaa agcaagaaaa tatgcagatt tactaaagga tatgcaagta tcttcactat
     5521 gtaatataaa gataggtgag tgtgaatgtt cgtttgaaaa ttatgaatgt tccatggaat
     5581 atttcaatga agtaataagc aataaaaagt atagaaatgt aaatataatt caaaaatata
     5641 gagcatcaaa caacataact aaaatattaa taaagactag aaattatcct gtcgcaataa
     5701 ataatttagc aaaatctgaa gtatatctag aaaaaattaa aaatcatagc ttaaaagaag
     5761 tagagaagtt tgatttattt ataagtttag ccatgtatta tgcaaaaagt aatgaaaaat
     5821 cttttgaaca atcaatatgc tatttagata gagctaaaaa catattaaat atgataaaaa
     5881 atatagaaga atatataata tatgatttag aaataattta tcaccaggca aagatatatt
     5941 atatctttgg atattatgat aatgccttaa acacaaataa aaaactcctt gaaaaatcta
     6001 aaaatgctaa aaattataat tatgtgaagt tagcatacaa gagtatatat ttgtgttttg
     6061 aaaagattca aaattatgaa atgagtatga aatactttaa aatgtattat gagttaaaaa
     6121 tgatgtatat aaaagctcga aatagaaatt atatagattc cttaaattat agacatgagt
     6181 atatagaaaa agaaataaat aacatgagaa aagtaaaact tgatttagat aaaaaaaagt
     6241 atgttgacca tttgacaagt gcatataata gaactttttt gaacgatttt ttagaaaaac
     6301 aagaagttag tgaatattgc acagcattta tgatagatgt agattacttt aaagaataca
     6361 atgatactta tggacactat aatggagata ttgctcttaa aaatataact ataataataa
     6421 aaaaatatct aaaaaaagac atgcttatga taaggtatgg tggagaagaa tttctgatat
     6481 tatctgtatg taaagattat aaaaaaagta aaatatttgg aagaaaatta tgtaggattg
     6541 taaagaaatt tttaaacaat aatttgacag ttagtattgg cattgataca tgtaaaaata
     6601 gtagtataga tataaaagaa ataattgaaa atgctgataa agctttatat aaagcaaagg
     6661 aaagtggtag aaatagatgt ttgcattatc atgattttaa gaatttttaa ctgttgaaat
     6721 aggattggtg atttacttga aaaggagttt aatagaagat ttaaagagaa atataaacaa
     6781 gatagaatac gatatgttga ctaaattcta tctaaatttg acagatttaa actacatctt
     6841 ggattatcac aaagaccttt taaatacaaa attagaagat aatagtagag tctatacttt
     6901 atatttaatt agtcttataa actatataaa aaaagataga gaagcatcat acaactattt
     6961 gatagagtca ttaaagtata ttaaaaaatt gtcattaaaa gatgattcag atataatatc
     7021 taaaatttat atttatctta ctttatcagc tatttctata agaaaatatg ataaggtaaa
     7081 taatttttat ttctcagcaa aaaaaattat aagacaaaat catttaaatg agttgttggt
     7141 attgttaaat atgagtttat gtgtagaaat aagtgcttta tatcagacca aaacggatgt
     7201 aatagcttta gtagaagaag tgtctttttt taaaacttta aaaaataaag ctcttgtaag
     7261 tagggcaaat tatatatttg ggagagttta tctatattta tttaataact ttataaaaag
     7321 tatggaatac ttaattgaat gtctaaaatt ggctcaagaa aataatttat actctataga
     7381 ggctttttgt aaatgtataa taagtttgtg ttatttagag agtggaaata atgtagaagc
     7441 tataaaatac ataaataatg tattagatgt tgcacacagt aataactcaa tatcagtaac
     7501 tgaaagagtg tctataaaaa tagaccttat atgggcatat ttaaaagaag atatgaataa
     7561 agaagcagaa gttattttat taaagactat aaaattaatg aaagttgttg aaggtgtata
     7621 taaggattat ttatattcat ttatattttt atattcagct gaaattgagt taaagaaaga
     7681 taattgtagt tttgaaaagg taaacaccta tttaatttta tcaaaaaata tatataaaag
     7741 ttttggagat gtttttatat ataattctaa actagattac atatataagt tgtatggaga
     7801 tttatatata aaatttggta atatagatga aggtataagt tgttatataa aaggtttaaa
     7861 tttagttaaa aagagttcat tgaatcttag aaaaatatct atattttatg ggctaatagc
     7921 aaatggatat gagctaaaaa aagattttaa actggcaata gaatattata aaagtatgga
     7981 ttggtattct gataggtggg aaaaaaataa ttcctataaa acatctcaag caattcacaa
     8041 acaatatgaa ctcagacaaa aacaagagag tttaagatta ctagtggatg ataataagaa
     8101 attagaaaat gatattttaa aagatggact tactaaacta tataatagaa gatatctgga
     8161 acagcagttg gatttattta atgaaaccag agacaaaaat atggtcgcaa tactggtcga
     8221 tatagattgt tttaaaagtt ataatgataa ttatggtcat ctagcaggag ataaagttat
     8281 agttaaggta actgatatta taagaagtgt atttatagat attaccgaac acataatcag
     8341 atatggggga gaagaaattt taatattagt agattctata gaatcaatcg gtgttgaaga
     8401 gattgataat atagaattat atattaaaag agttttcaaa cttcttgaat ttgagggtat
     8461 tgaacatata tattctaaaa taaaaaacta tataactata agtgctggag taagtataaa
     8521 aagatgtcgt agtagaaaag atgttgaaat attaattgaa gatgcagata agaacctata
     8581 taaatcaaaa aaagcaggta gaaatcaata cattataatt taaaattgag gtgattttgt
     8641 tgcgaaaaaa aataggaatt atagcctctg atattgaatt aaaagaaaga atagaagaat
     8701 tatatagaga agatgtagaa aatggaacta taataatcga catacttaac ctagacttaa
     8761 tggaaaatca aggtcgtata cttgttgaaa aaggtgccca agctataatt ggtagaggtg
     8821 gaggttatag tttagtaata gatacagtaa atgtacctgt tataccaatg aatatgaagt
     8881 caactgacct tttaagagca atagaaatag caaagaaata ttctaaaaaa gtagtattga
     8941 ttcttggaga caatgaagtt tcttttgact atgttggctg gagaaatgtc ataagtactg
     9001 aaataactga agaatggttt gaatctaagt atgagataag aagtaaagtt gtaaaatata
     9061 tagaccaaaa agatgaaatt gtaatcgtag gaggaggttt ggcatgttct tttgcaaggc
     9121 aatatggaat tgatagtgta tttgcaactg ctagtgatga atctataaga gaagctgtag
     9181 aatattgtaa aaaattatta gatacattag gtgaagaaaa gtttaataat gaagtgttac
     9241 gaaatatact tgatggtata aaagatggag taattgctat agatagtaat ggcagtataa
     9301 ttttgtacaa tgaaagtgca aaaaacatgc ttaaagtaga gagaaaatgt gcacttaata
     9361 aatacatctt agatgtattt cctaaaatgg agtggatgtt agattgttta catgaaaaag
     9421 aggacgtaga agatagaaag ataagaaata taaataatct aattgttaat actaggacaa
     9481 cattgataaa agttgataat agtacttatg gagttttagg aataatacaa gatataacca
     9541 agctacaaaa cttagaaaga aaaataagat ttgacttgaa tcaaaaaggt ctctatgcta
     9601 gatatacttt tgatgatttt ttatttaaag ataaattaac taaagaattt attgaagaag
     9661 ctaaaaaaat tggaaaaagt gattatacaa ctttacttta tggggaaagt gggtctggta
     9721 aagaaattat agctcatagt atacacaata taagtaaaag aaaagatagg ccttttgttg
     9781 ctataaattg tgctactata gcagaaaatt tacttgaaag tgaacttttt ggctatgaag
     9841 aaggtgcatt tactggtgct agaaagggtg gaaaaagagg gctatttgaa cttgctcatg
     9901 gagggactct ttttttagat gaaataaata gtctatcctt taatattcag acaaaactat
     9961 taagagtaat agaggaacgt caaataatga gaattggttc tgattatata atacctctgg
    10021 atataaggat tatagcagca acgaacgaat ctttaaccga aaaaatagtt atgggtacat
    10081 ttagagcaga cttattttat agattgagta gtttagagat aaatatacct cctttaagag
    10141 acagaagaga agatataata cctttgttta acaattttgt aaatgaagta ttaaaggatg
    10201 atggtctcaa tggaataaac agtatagatg aaaattttgt tttgactaaa gatgaaatgg
    10261 ataagctata taattatagc tggcctggaa atgtaagaga gttaaagaca atagctcaaa
    10321 aatatgttgt gacaggtaaa attaaattga gacaagatag agattttaaa actaaaaaat
    10381 tacttcctaa ctctgaagta gacaaattta attctgaaac gacagcaagt gtagaagtcc
    10441 aagatgaatc tataaatata agtaagataa atgatggtaa gataagtata gatataaaag
    10501 aagtaaataa gtatgttgaa gaaaaaataa taagtatgct ttttgctcaa ggactttcta
    10561 aaaatgaagt tgcacaagtt cttggaataa gcaggacatc tttatggaag aaatacaata
    10621 aaaatattta aatataatta catataaatt attttgaagt aatgttaatt ttttaaaaca
    10681 agtgttaaga aaaattaaca ttactttttt gcaaattttt atttttttag acaggcatat
    10741 atatttataa gaaaaatagt taaaacactt aatttaataa tgaaaaaaat aataattcta
    10801 aacaaaatca attttacata aagtgggatt tattggcata gtaattgctt aatatataat
    10861 gtaagagtaa atacaatttt aggggtgatg gtatgagtca aagtatggtt ggtaagcatg
    10921 caatgtggcc aaaagaaaat gatgttatat ttagcatatc tggcagagct caagcagcag
    10981 agaaggcttt tggtatggat aatgtaataa atgcaacaat aggcgctcta atggatgatt
    11041 caggaaaatt gataacaatg aaaactgtat atgaagaata taaagcatta gataactgtg
    11101 agataggtgc atatgcagct cttgaaggtc aaccagatta cttagaagca gttaaaaaag
    11161 tatttttcag agattactta ccagaaggac atataagagt attagcttca ccaggaggaa
    11221 gtggtgcaat aaaattagct gtatggaact atactaatga aggagatgag gtattaacat
    11281 ctgattggtt ctggagtcca tatgtaagta tagctgaaga ggctaataga aaagtagtta
    11341 attaccaact ttttgatgaa aatagaaggt ttaattttga gtcgtttaaa gaaaaatttg
    11401 taaatatagc agaaaaacaa ggtagagtct ttacaattat aaacacacca gcgcataatc
    11461 caactggata tagtgttgca gatgatgagt gggataaaat attagactta tcaaaagaag
    11521 tcgcaaaaga taaagacaaa aagataatat ttttcgtaga ttcagcatat attgactttg
    11581 caggtgatga tgatgtatgt agaaagtttt ttaagaaatt ttctaatttg cctgaaaacg
    11641 ttctcgtatt ggttggattc agtatgtcaa aaggatttac agcttatggt atgagaatgg
    11701 gggctataat ttgtatatct tcaagtgaag atgtagctga agagttccat tactcatgtg
    11761 ttcattcatg tagagcaaat tggtcaaatt gtaatagaag tgccatggca gtattatcta
    11821 acattgtaaa tgaccctaaa aaattcaaag aatatgagga tgaaaaagaa atatacaaaa
    11881 acatgcttac tagaagagca gatgtatttg taaaagaagc tgaaagagta ggattagaaa
    11941 ttcttccata tatagcaggt ttctttgtaa gtataccatg tgataatcca aaagaagtat
    12001 gtgaagaatt gactaaacat aatttatttg cagttccatt aaaaatgggt ttaagatttg
    12061 cagtttgtgc agtttctgaa gataaatgta agaaagcacc aagtattata aaagaagcac
    12121 ttgaaagttt agaagtaaaa ataaataatt aatttcattg aacaaattgg gtggactatg
    12181 aatttaataa ttgtagatgg agggaatttt atatgaaaca attgatgaca ggtaatgaag
    12241 caattgcacg tggagcctat gaggctggtg ttaaatatgc atctgcatat ccagggactc
    12301 caagtacaga aatattagaa aatatagcaa catataaaga tgcaatagtt gcagagtggg
    12361 ctccaaacga aaaggtagca cttgaggctg ctataggggg ttcaattgca ggagcaagaa
    12421 caatggcttc tatgaaacat gttggtttga atgttgcagc tgatccaata tttacttatg
    12481 catatacagg ggtaaatggt ggtatggtac ttattacagc agatgagccg ggaatgcatt
    12541 catctcaaaa tgagcaagac aataggatgt atgcaaaatt tgcaaagata cctttatttg
    12601 agccatcaac tagtcaagaa gctaaagata tgataaaaga agcttttgaa gttagtgaaa
    12661 aatacgatac accagtttta tatagagtta caacaagact ttgtcattca aaaggattag
    12721 tggagtgcta tgatagagaa gaagttgaaa ttaaagagta tgttaaaaat gctaagaaaa
    12781 tggtaactgt tccagcaaat gctcaaataa gaaggggcgt tgttgaagaa agaatggaaa
    12841 ttcttaaaaa attctcaaat gaaactgatt taaactacta tgaaataaat gatacaaaaa
    12901 taggtgttat agcctctggt atgtgctgta attttgccaa agaagttttt ggaaaaaatg
    12961 catcatatat gaaactagga tttacaaatc cattacctta tgaaaaaata aaagaatttg
    13021 cagaaaaagt tgataaaata tatgttatag aagaaaatga cccatttata gaagaacaaa
    13081 taaaagcata tggaatagat tgtattggaa aagatgtaat accaccatat ggagaaatga
    13141 caccagatgt tttaagaaaa gctatatttg gaaaaacaaa tgatactata gaatacaaat
    13201 ctgaattagt aacaccaaga ccaccaagtt tctgtgcagg atgtccacat agaggattct
    13261 tctatgaact tggaaagaga aaaaatttaa tagttggtgg agatataggt tgttatactc
    13321 ttggatttgc tcctccatat aatggaatag attatgttgt ttgtatgggt tctgcttttg
    13381 gaactgcaca tggagctcaa aaagttctta atatgaagga tgataatgaa aaaagattgg
    13441 taggtgtact tggagactca acattcttcc atacaggaat aaatggactt ttagatgttg
    13501 tttacaatag aggtaattct atttcggtta tattagataa tagaataact ggaatgacag
    13561 gtcaccaaga aaacccaggt tcaggatata ctttacaagg tgctaaaact aaagaagttg
    13621 atatagaagg attagttaaa gcttgtggaa tagaacatgt aagagttata aaccctaata
    13681 atttaaaaga agtaaatgaa gcactagatt gggctttagc tatagaagat gaaccatcag
    13741 taataataac aagatggcca tgtgtactta aaaaattctc taaagaagat atagaagaat
    13801 ttaataatcc atttaagact aagtgtaaag ttgaccatga taaatgtata ggatgtaaat
    13861 tatgtttgaa aactggatgt ccagctctat catttgataa agagaataag ttatcaaata
    13921 tagatagaaa tcagtgtgta ggatgtggag tatgtgcaca agtttgtcca aaacaagcaa
    13981 tagttaagga ggaaaaataa tatgactaag agtatacttt tggtaggtgt aggaggtcaa
    14041 ggaactatac ttgctagtaa attattaact atgggattga tggaagctgg atatgatgta
    14101 aagatgagtg aaatacatgg aatgagtcaa agaggaggtt cagtttcctc acaagttaga
    14161 tatggagatt gtgttcattc tccagttata gaaattggtg gagcagatat attagtatct
    14221 tttgaaaaaa tggaagcttt aagatggttc aattatttaa aaccagaagg aaaagcttta
    14281 gtaaataatc ataggataga ttctatgact gttttaatcg gaggagctga atatcaagaa
    14341 agtgatatag atgcagagtt aagtagatta aatgcaaaag ttataaatgc agctgataaa
    14401 gctgaagaac taggaaattc aaaaataatg aatgttattt tattaggatg tctagttaaa
    14461 tctatgcaat tagagtctat tgattgggaa aaaattataa gtgaaaatgt taaaccaaaa
    14521 tttgttgaat taaacataaa agctttccat gaaggtatgg aaatggttag caaatagttt
    14581 ataatagata taaatagact atagaaatta aggtgaaagg aagaaaatta tgacttacag
    14641 aatattagcc ataaatccag gttctacttc tacaaaaata gcagtatatg atggagaaga
    14701 acaaattctt gtgaagacga tagaccatcc agctgaagag attgcaaaat ataatactat
    14761 acaagaccag tttgaaatgc gtaaggaagc agttttgaat attcttaaag aaaatagtat
    14821 agacttaaaa tctcttagtg caatagtagg aagaggtgga gttttaccac cagtaaaatc
    14881 aggagcatat ttagtaaatg aagaaatgat tgatgtacta agacatagac cagtacttga
    14941 acacgcttcc aatttaggtg ctgttgtggc acatgcaata tcagaacctc ttggaatcaa
    15001 ctcatatatt tatgattctg ttgcagtaga tgagcttata gatgtagcga gaatatctgg
    15061 actttgtgga atggatagat caagtgcagg gcatgcatta aatactagag caatggcttt
    15121 aaaatatgct aaggataaag gaaaagatta taagagctta aacttaatag tagctcacat
    15181 tggtggagga gtaagtattt atcttcatga aaaaggaaga atggttgata tgctatctga
    15241 tgatgaagga ccattttctc cagaaaggtc aggaagagta cctgctacaa aattagtggc
    15301 tgcctgttat tcaggtcaat attcagaaag agaaatgact aaaaagataa gaggtaaagg
    15361 tggtatagtt tcatacctaa atactgtaga tgctagagaa gttgaaaaaa tgatagcaga
    15421 aggaaatgaa gaagcaaaaa ttatttatga agcaatggct tatcagttag caaaaggtat
    15481 tggagagtta gcaactgtag tagatggaaa ggtagatgct ataattataa caggtggaat
    15541 tgcatattct gaaatgttta cttcaatggt taaaaagaaa gttgagttta tagcaccagt
    15601 agaaattatg gcaggagaaa atgagttgga atcacttgct tttggaactt taagagtact
    15661 aaatggagaa gaagaagcta gaatttatag tgaaaattaa taagaaaaac taaaaatgtt
    15721 tatatactaa aaaagtggta tatccgtttt ttatacacga aagcttagag tataggtgaa
    15781 aatgtataaa atttggattg ccacttttta taattaaaat tttatcttaa cctaataaat
    15841 aattctgtaa aatttcgtat ttatatatat attttattgg tatttattgt tattattatt
    15901 atgaggagtg atttttgatg ggaaaaataa gtttaaaaag tatgataaga ttattttcag
    15961 gtttttttat atttgcaatt tcttctgtgc ttatgattaa tgcacatgta ggtcttatgc
    16021 cttgggatgt acttcaccaa ggtttatcta taaagctagg cataactata ggtcaggctt
    16081 ctataatggt aggagttgta attgttatat tggatgcagt ttttggtgaa aatataggat
    16141 ggggtacttt actcaacatg acatttatag gaatttttat tgatttagta atattttctg
    16201 gagtgatacc tcatgcaagc aacacttata taggtgtgtt tatggttgtt ataggaatta
    16261 ttttggcagc tatagcaagt tttctatact taggtgtttg tttaggaagt ggaccgagag
    16321 atgggcttat gattgctctt cagaaaaaaa ctaataaatc tgtacgtctt gttagaacaa
    16381 ttcttgaaat actagcatta gttgttggat ggttattagg aggttctgta ggtattggta
    16441 cgcttgtaag tgctttaggt ttaggatatg tattacaaat agtgtttaga atatttaaat
    16501 ttgacacaaa attgctgaaa catagattta ttatagatga tataagagaa tggaaggaaa
    16561 agaaaagcaa tgaacataaa tgtaaatcaa gtattgtaat aaaaaatgag cagaattaat
    16621 tatgctaaaa tatatttgta gtttatctaa tttatagtaa agtcaaggag attttgaaat
    16681 ggaattatgg gatttatata atgctgatgg aattaaaaca ggaaatgtta taaaaagagg
    16741 taattctatt gaagaaggtt attaccattt ggcagttgaa gtgtggattt taaatagtaa
    16801 ctctcaaatt ttaattcaga aaaggtctaa aagtaaaaaa acattgccaa atatgtgggg
    16861 aatgacaact ggatgtattg tttctgggga agacagttta gaaggagcaa ttcgcgaagc
    16921 aaaggaagaa atagggatag atataactaa agatgagatg aaagttttta gaagtatgat
    16981 tcatgaagat actctttggg atgtatactt agttaaaaaa gaatatgata tatctaaagc
    17041 tattttacag gaggaagaag taagtgatat aaagtgggta agcacagatg aaataagaca
    17101 attactaaaa gagggtttgt tttttgaata tccagagata tacgaattgc tatatgagat
    17161 agataataat aaattaaata tatgatgtag agcagatttc agtataagta agattattta
    17221 aaatgtatct tctctaaatt ttaatattgt tttatgaaaa ttaataagtt atttttagta
    17281 tttttctact tgttttttga gattaaaata aaaagggcag tcattataaa gtcataaaaa
    17341 tttggggagg atttaaacta tggaagataa caaaggattg gcacgtgaca gttcaaaata
    17401 taaaattgtt tctatatctg tcgtagtaat tattctaatt acattttggt atatgttaaa
    17461 tatggtgctt ttaacattta ttatgacttt tattttctat aacttgcttg tagccacaag
    17521 aaaacgaatt aagaagtttt cttctttaaa tattccagat tcattgataa ttatagtgtt
    17581 atatgcacta tttgctatat tgttagtatt gattagctat gcagttgtac caataattat
    17641 agttcaactt acagagttga gtagggtttt tagtgatttt gatgtaaatc aatttgctca
    17701 atctttagga ccgaaattgt atcctattgt ctcaaagctg gattttaata aatatatttc
    17761 acaagcaggg ctacttatag catcaacagc aacaaaagtt ggaagttttg gagtaaatat
    17821 attattggca tttttactga gtttactcct tttacttgaa aaaaatgaaa ttaaaaattt
    17881 tggagataaa ctttctgata gtaagatttc ttttatttat aattcgcttg tattttttgg
    17941 aaagagcttt gttaaaaatt ttggtgaagt aatgaaagta caagttatga ttgcctttat
    18001 aaattctgtt gtttctatga ttttcttagg tttcatggga ttccctcaaa tatgggcttt
    18061 aggatttatg atttttgtgt tgggtcttat tccagtagca ggagttatag tgtctttgat
    18121 tccattaact gtaatagcat ttaatacagg aggaataaca aaggtttttg gagttttact
    18181 tatgatttgt atagtgcatg cagtagaaac atacatatta aaccctaagt tgatgtcgaa
    18241 tagaactaaa cttccagttt gttttgtttt tattatttta cttgtaggag agcattattt
    18301 aggtgtatgg ggattactaa ttggagttcc aatatttatg ttccttatgg atattctggg
    18361 tgttaaattt acttctaggt aatatattaa aataataatt tatttttata tataaattat
    18421 gaattgtttt aaaaaagaac ctataaatag acatcattaa taaagacatc tatttgatag
    18481 gttcaatttt gctttgtata aatactataa aactaaataa aaagaaattt atttcatttc
    18541 aaaagtttca caacttgcat ttctttcatt tatatgaact ttttcagctt tgcaaagttc
    18601 atttttattg tatttacatt ttacagcttc acaatgaatg ttatctgtat caacttggtt
    18661 agtttcatta gaacgactag ttataccaga tgtatatttg tcttcaaaag aagcacaata
    18721 agtattttct gtagtagttg cttgcattcc atctactttt atactaccag cataacaata
    18781 tccactagta ttataagcac aattagtagc agcacaattt aaattattat ttttcataaa
    18841 taacctccta agttttattt aaacttatta tgtgaagtta tctaaatttt atacacttat
    18901 ttacaattac atttatcaca tttaacaact acatctttgg aagtttccct tcctaaattt
    18961 ttattaacat gatacacatc aacattttca gttgatccac atatagaaca tttaaaattg
    19021 ttttctagta gggttctttc tttaaataac ttccaataca tagaattgat gtaatcatta
    19081 taatttacct tgaggcatgt ctcaagaaag tttatgtata gattgtctaa atctcctgta
    19141 tttaattctg tatattctgg ttcaaaatat tcacaacatc ctctaaaatc gggctttgta
    19201 tctgaaataa cacatctaat atcatatcta tagtctaggc taggcccctt aaatgaaaga
    19261 tgtgtgcaaa aagaacaaga tatttctggt agattttttt caagttcatg taatttatta
    19321 ttcataagta ttcctccaaa aaatttattt taagtatata aatgaataga ttattttaaa
    19381 acttttattt atttatatta gagatttagg ataaatattc caataaggag taaatactta
    19441 agtatttatt actgtattaa ataaaaaaga ggcaaatcta acttgagatt gcctactttt
    19501 ttatcatttt ttaataaaat ttattctaag tattatttta tttagattta gtcagccaac
    19561 ttagtatctg ctttgctttt taactttcta ccagagcgta cagttaaaat agcatataat
    19621 acagtaaata tggcagctaa aacatatgat atatttatgt ttaagttaaa tccaatttga
    19681 gcattaaata tataactgaa tataacaaag gaataaaaca taccaggaat taagcttata
    19741 atataattct tttcttttat tataaggtaa actgtaatca ttgcaaatgc aaatatggca
    19801 atagtttgat tactccaaga gaagtatctc cacaatatat taaatccaga agcactaagc
    19861 tttgcaaata taaggatggc tatgacagga ataaatatac agattgacaa tataacacga
    19921 tgtttctttt ccttttggtc ataatgaaag taatctgcaa gcattaatct taaagatctt
    19981 aaagctgtat ctccagaagt tattggtaaa acaattacac ctattatagc tatgatacca
    20041 ccaatagaac caagtaaatc tcttgcaacc aaacctatta catcaggaga acctactaat
    20101 tctttaggta ttcctttatt gtaaataccc atagcagctg cagcccaaat cattgcaatt
    20161 aaaccttcta aaatcatcat attataaaaa gtagtttttc cttctttttc atttgaaact
    20221 gatcttgcta taagagtagc ttgagttgag tggaatccag aaacaatacc acaagcaact
    20281 gtaacaaaga atgtaggaat aagaggaata ccatcaggat gaataccttt ccaatttgca
    20341 agacttaaat tagctaaatc ataaccttgt gtaaagattc caattccaac accagcagca
    20401 gataaaagta gaatagctcc aaatattgga tatacctttc ctatgatttt atcaattgga
    20461 aatagtgttg cacacaagta atatagaaag ataagtccat aaactatcca gacaactgga
    20521 ttatttatag tcgatttcat atttaaaatt tgagtaacaa ctaaatcccc aggtgtataa
    20581 ataaaaactg caccaactaa aagcattaaa agacaaagga atatattata gacttgaaat
    20641 actttattac ctaaaaatct actgataaga gaaggcattt gtgctccttt ttctcttata
    20701 ctaatcattc cactcatata gtcatgcaaa gctcctccaa atacacagcc gataggtatt
    20761 agtatgaatg ctattggacc aaacaatata ccttggatag gtcctagaat tggtcctgta
    20821 ccagctatat taagcaactc aattagacta tttttccatt ttttcattgg gacatagtct
    20881 actccatcac tttgagcaag tgcaggtgtt tttctgtcat ctgggccaaa tactttttca
    20941 caataagcac catataaagc acctcctatt agtaatatag ccaagccgat taagaatgtt
    21001 gtcataatat caccccattt ataaaatttt ttggtctttg tatacatatt atctaaatgt
    21061 gtagatttat attgaattta attctggaca acctacaaat tctataaagt attacaacta
    21121 atataatttc agaaataaat acatatacat aaccatgtac ttagtataca ggtatttggt
    21181 aatattttca atgttatagc aataaagaat ataaacaaaa ttctgataat attttacaaa
    21241 aaaattaaaa aatataaaaa ttagtttaaa tagatggaga aaaaaggaaa atcaattgag
    21301 aatataaatg tctatatctt aacaatatta agaggtttaa aattcttgaa gaaagaatag
    21361 tcaagtgata taatgggttt acaggtgtat atacactagt acatatagaa agcggtgcaa
    21421 ttatggataa agatttaaca tatcaaatca aagagctaaa gaaagaaaaa aatgcaataa
    21481 tacttgccca tttttatcaa ccaccagaaa tacaagaatt agcagatgca gttggtgact
    21541 catattattt aagtgagatt gctagagatt gtaaagaaga agtggttgta ttttgtggag
    21601 taaggtttat gggagaaagt gctaagatat tatcacctga aaaaactgta ttgatgccag
    21661 taagtaacgc tgggtgtgct atggcagata tggtagatga agaaggcgtg ataaaattaa
    21721 agcaacagta tccaaatgct ttggttgtat gttatataaa ctctacagct aaagttaagg
    21781 cacattgtga tgtttctgtt acatcttcta gtgcaattaa aatactagaa aacatagata
    21841 ataaagaaat tatattcctt ccagataaaa atttaggagg atatattgca gaacaatttc
    21901 cagataaaaa ctttatattt tgggacggat attgtaaata ccataacaat ataagagctg
    21961 aagaaataat tgagttaaaa gataagtata aaaatgcaga agtattagta catccagaat
    22021 gtaaaaaaga aataagagat ttaggagatt atgtaggtag tacatcagga ataataaaat
    22081 atgctactaa tagtaaaaac aaagacttta taatagcaac tgaagaaggt atattacatg
    22141 aattaaagaa aaataatcct aataaaaact tttatattcc tggtggaaaa atactatgta
    22201 cagatatgaa aaagacaaca ttagaaaatt tatatagtac acttaaaaat atggaaaatg
    22261 aagttattgt agaagatgaa atcatggaaa aggctttaaa ttcactgctt aacatgcaca
    22321 aattagcaga aggatagagg gatagactta tgaatttaga acaagatgtt ttaatagttg
    22381 gaagtggagt ttcaggatta tactgtgcat taaatttaga taaaagttta aatgtgctag
    22441 tagtatcgaa atcaacaatt gaaaacaaca atacatatct tgcacaaggt ggaatttcaa
    22501 ctgctagaaa tattgatgat atagaatcct ttgtagaaga tacaatgaag gctgggcagt
    22561 ataaaaatag agttgaagca gttcaagtac ttgcagatga atctatagaa aatgtaggtc
    22621 agatagtaga atatggtatg cctttagaca aagaaaatgg agagatagat tacacaagag
    22681 aaggagcaca tagtgtaaat cgaatagttc atagtaagga taatactgga gaagttgttt
    22741 ttaaaacttt attaaaagaa gctaaaacta gagaaaatat aactcttata gaagatgcat
    22801 atcttttaga tatattaaaa gatggcaata aatgtatagg agctagaatt tttaaatcta
    22861 aaaaagagat tcatgttttt tcaaaaatag tagttttagc tacaggtgga attggtgggc
    22921 tttttaaaaa ttctactaat caaagacatc ttacaggtga tggaattgca atagctttaa
    22981 gaaataatat aaaaatagaa aatttagaat atattcaaat acatcctaca gccttttatg
    23041 aagaaaataa tgaaggtagg agaatgctta tatctgaatc attaagagga gagggagcta
    23101 aacttttaaa taaaaataaa gaaagatttg tggatgaact tcttcctaga gatgtagttt
    23161 caaaagctat ttttgagcaa atggaaaaag ataaattacc ttatgtgtat ttagatgcaa
    23221 cacatttaga tagtgaatat ttaattaata gattttcttt tatttataat gaatgtttag
    23281 ctagaggaac agacataacg aaagaatgta taaaggtatc tcctgcacaa cattatttta
    23341 tgggcgggat acatgtagat ttggattcaa aaacatctat ggaaaattta tatgcagttg
    23401 gtgaaattag ttgtacaggt gttcatggag ccaatagatt ggcaagtaat tcattacttg
    23461 aagggcttgt attttcaaaa agagcagcta aaaatataaa ctcagttata gataatgtaa
    23521 aagttaagtt tatagatgta ccagatatgg atatagatat agaacaggta aaaaaagaaa
    23581 ataaaatatt agtcataaaa gaaattgaaa gaacaagtga ggattttggc gatgaattat
    23641 ttgattattg atagaatgat acaggatgct ttgatagaag atgttccaag tgaagatata
    23701 acaactaatt ctattgtgga tgaaaattct aaatctactg tagatttaat ttgcaaacaa
    23761 gatggtataa tagctggctt aggtgtattt aagagagttt ttgagatact tggtgatgta
    23821 gatgtaaaat tatataaaaa tgatggtgat agagtaaaga atcgtgaaaa gatagctgta
    23881 ttaacaggta gtactagaaa cttacttgta ggagagcgtg tagcattgaa ttatttacag
    23941 agaatgagtg gtatagctac tattacaagt aggtatgttg aaaagcttga aggtacaaat
    24001 acaaaattat tagatagtag aaaaacgata cctaatctta gaatattaga taagtattct
    24061 gtcaaagtag gtggaggatg caatcataga tttaatttat ctgatggaat attgcttaaa
    24121 gacaaccata ttggggctgc tggtagtgtt aagaaggcag ttgaattagc tagaaaaaat
    24181 acttcttttg ttagaaagat agaagtggaa gtggaaactt tagacatggt aaaagaagca
    24241 atagaagcca atgcagatat aataatgttg gataatatga gtttggaaat ggcaaaacaa
    24301 gcagtagata ttataaatgg aagagccatt gtagaatttt ccggaaatgt aaatttagat
    24361 actatagaag atataggaaa aattggtgtg gatgttgttt ctgtaggagc gcttacacat
    24421 tctgttaaag ctctcgatat aagtatgaag aatttgagaa atatataaat tttatattga
    24481 aataaactta attaaaatag actccttaaa tttgttttat aaat
//
LOCUS       DAMJVI010000051        24473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.053, whole genome shotgun sequence.
ACCESSION   DAMJVI010000051 DAMJVI010000000
VERSION     DAMJVI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.053"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            113..1399
                     /locus_tag="PC545_002146"
     CDS             113..1399
                     /locus_tag="PC545_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2456913.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            1511..1984
                     /locus_tag="PC545_002147"
     CDS             1511..1984
                     /locus_tag="PC545_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1643..1645,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HDF2456914.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            2016..3326
                     /gene="grdB"
                     /locus_tag="PC545_002148"
     CDS             2016..3326
                     /gene="grdB"
                     /locus_tag="PC545_002148"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3063..3065,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2456915.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            3582..5114
                     /locus_tag="PC545_002149"
     CDS             3582..5114
                     /locus_tag="PC545_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HDF2456916.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIAGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            5125..6270
                     /locus_tag="PC545_002150"
     CDS             5125..6270
                     /locus_tag="PC545_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HDF2456917.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFDDIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
     gene            6580..7659
                     /locus_tag="PC545_002151"
     CDS             6580..7659
                     /locus_tag="PC545_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDF2456918.1"
                     /translation="MSRVKNVVELLETKGVDALYLTKKTNVNYISGFPDEEAYAVICK
                     DGNFLVTDSRYMELAEKVCKDFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYVKDKDEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATRLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGGASEKQLEIYNLVKEAQNVGVENMKAGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEEGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            7772..8371
                     /locus_tag="PC545_002152"
     CDS             7772..8371
                     /locus_tag="PC545_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456919.1"
                     /translation="MYKKHKFIKNIKKAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSANFIKRPLRKYTNTDVSSNLFIDNNLSPKGQE
                     SNQNNIMNVKNTIGFTSEADNYEKKIPINNNEGFEEGETSELQIIIATVICIVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSKPD"
     gene            complement(8558..9430)
                     /locus_tag="PC545_002153"
     CDS             complement(8558..9430)
                     /locus_tag="PC545_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2456920.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISNYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTENLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
     gene            9847..11250
                     /locus_tag="PC545_002154"
     CDS             9847..11250
                     /locus_tag="PC545_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456921.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISSDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASIKDKKEILKVVDPEGKLDVNTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVVNFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            11277..12821
                     /locus_tag="PC545_002155"
     CDS             11277..12821
                     /locus_tag="PC545_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2456922.1"
                     /translation="MNNEGAFEELSGRLRNKELCDLIMNADEAAKIIEDGMVIGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEEGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGVVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKSGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            12852..14243
                     /locus_tag="PC545_002156"
     CDS             12852..14243
                     /locus_tag="PC545_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HDF2456923.1"
                     /translation="MEKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKVAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKVQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            14436..15932
                     /gene="abfD"
                     /locus_tag="PC545_002157"
     CDS             14436..15932
                     /gene="abfD"
                     /locus_tag="PC545_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HDF2456924.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSVDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKKIAGIKKEEALNL"
     gene            16067..16342
                     /locus_tag="PC545_002158"
     CDS             16067..16342
                     /locus_tag="PC545_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456925.1"
                     /translation="MTCGVRFCGGCNPRYERGKALTIIKEHFNGRVDFSIAQENVEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            16366..17673
                     /locus_tag="PC545_002159"
     CDS             16366..17673
                     /locus_tag="PC545_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HDF2456926.1"
                     /translation="MSWQELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPAFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSEFDYIVEKSQPLFELQPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFANNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAVEFEKRFKEKY"
     gene            17693..18811
                     /locus_tag="PC545_002160"
     CDS             17693..18811
                     /locus_tag="PC545_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2456927.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVSPKKVKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     SQIDEFTDSTIANQQRLLANNYVELSREEIREIFANLY"
     gene            19098..20018
                     /locus_tag="PC545_002161"
     CDS             19098..20018
                     /locus_tag="PC545_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDF2456928.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLLIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPPRYEDVAYEVKEEEK
                     TDESNEVDTIINKGMNYLTQIKEANKNIKSESMGDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            20133..21290
                     /locus_tag="PC545_002162"
     CDS             20133..21290
                     /locus_tag="PC545_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2456929.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTNDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(21371..22267)
                     /locus_tag="PC545_002163"
     CDS             complement(21371..22267)
                     /locus_tag="PC545_002163"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2456930.1"
                     /translation="MLKKVKYPIGQVKVSDKFKTIKLLSRKYSISSMCKLISVSRSGY
                     YKWLSYSKKSSDRGIKDRIIKDYIIEIHKKYRGTYGRKRICTYLNKILDTPINHKRVY
                     RLMKELGIKSIIRKKVYRRKFKSYEIYDNILNREFRANQPLEKICMDITYIPIGKKFL
                     YMNVAKDLFNGEIVAYEISTKMDTKLVNNTVNQLINMNLAKDCILHTDQGSQYTSRSY
                     SKRLKDNGIIQSMSRRGNCWDNAPIESFFSHFKSELIYLIDTTDPKEMISLINDYIYF
                     YNNERIQLKNGMSPIEYRTHSE"
     gene            complement(22225..22542)
                     /locus_tag="PC545_002164"
     CDS             complement(22225..22542)
                     /locus_tag="PC545_002164"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2456931.1"
                     /translation="MSKKHKKYSKELKLKAVNLYIKEGYSSYKIAEMLNIRSKTQVQN
                     WVKDYKNKGKTAFDDETRGRFKNISLENDNRKFKSVEEELKYLRMENEFLKKLSTLLD
                     KSK"
     gene            complement(22679..24178)
                     /locus_tag="PC545_002165"
     CDS             complement(22679..24178)
                     /locus_tag="PC545_002165"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDF2456932.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIEDEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
BASE COUNT         9319 a         2949 c         4528 g         7677 t
ORIGIN      
        1 gcataaaaac acaataatat atctttaaaa aaataagata aattgtaaaa tgatataatt
       61 ttgagaaaaa ctcaaatgca tatacacaaa ttaaataaca ggaggtgcaa gtatgcgtct
      121 tgaattaggg aaaatcttta taaaagatat tcaatttggt gatgtaacag aagtaaaaga
      181 tggagtatta ttcatcaata agcaagagat gctacaagaa attggtggag atgagcatat
      241 taaatctata gatatagaat tagctcgtcc aggtgaaagc gtaagaataa cacctgtaaa
      301 agatgttatt gagccaagag taaaagttga aggtaatggg ggaatcttcc ctggtatcat
      361 gtctaaagtt gacactgtag gagaaggaaa aactcatgct ttaaaaggtg tagctgttgt
      421 aactactgga aaaatagtag gtttccaaga aggtataata gacatgactg gagaaggagc
      481 taagtacact ccattttcta aattaaataa tgtagtagta atagctgaac ctattgatgg
      541 tttaaaacaa tacgctcatg aaaaagctgt tagaatgata ggattcaaag cagcaatgta
      601 cttaggagaa gtagcaagaa atcttactcc agatgaagtt tcagtatatg aaactaaacc
      661 tcttcttgaa tcaataaaag aatatccaga gcttccaaaa gtaggatatg tttacatgct
      721 tcagactcaa ggtttattac atgatactta tgtatatgga gtagatgcaa aacaaatagt
      781 accaacttta ttatatccaa cagaattaat ggacggagca attgtaagtg gtaactgtgt
      841 ttctgcatgt gacaaaaacc caagttatgt tcatataaac aatggggttg ttgaagactt
      901 atatgcaaga catggtaaag atataaactt tgtaggtgta attataacta atgaaaatgt
      961 ttatctagca gataaagaaa gatcttcaaa ctggacttct aaattatgta aatatttagg
     1021 attagatgca gttatagttt cacaagaagg ttttggaaat cctgatactg accttataat
     1081 gaactgtaag aagatagaaa tgcaaggtgt taaaacagtt atagttacag atgaatatgc
     1141 tggacgtgat ggaggttctc aatcacttgc agatgcagat gtaagagctg atgctgttgt
     1201 aacaggtgga aatgccaacc aagttgtagt tcttcctaag ttagataaag ttattggaca
     1261 tttagaagta gtagatgtaa tagctggtgg ttctgatgga agtttaagag ctgatggaac
     1321 tattgaagtt gaaatccaag ctataacagg tgcaactaat gaaacaggat ttggtcattt
     1381 aactgctaaa ggatattaag aagtatttta ttaaacgatt gagtaatgtt aataaaacat
     1441 attatagata attataataa gtgttataga tatataaaaa tatattagaa atataataag
     1501 gaggattatt atgagtttac ttagtaataa aaaggttctt ataataggtg accgtgatgg
     1561 tataccagga cctgcgatag aagaatgtgt aaaaacagta gaaggagcag aggttgtttt
     1621 ctcatctaca gaatgctttg tctgaacagc tgctggggct atggacttag aaaatcaaaa
     1681 cagagttaaa gatgctgctg ataaattcgg agctgaaaat gttgtgattt tactaggtgc
     1741 tgctgaagcc gaagctgcag gtcttgcagc cgaaacagta actgctggag atccaacttt
     1801 cgctggacca cttgctggag ttgccttagg attaagtgtt taccacgttg ttgaggaacc
     1861 aataaaatca ttatttgatg aaagtgtata tgaagaccaa ataagtatga tggaaatggt
     1921 tttagaagtt gaagaaatag aagaagaaat gtctggtata agagaagaat tttgtaaatt
     1981 ttaatgccta aaagcaaaag gggtgaaact aagagatggg aaaactaaaa gcagtacatt
     2041 atattaatca gttctttgct ggtataggtg gagaagaaaa agctgatact aagccacatg
     2101 tggcagaaac tttacctcca ataagtttac aacttgataa attattaggt gaagatattg
     2161 aaatagttgg aacagtagtt tgtggagata gctatttcaa tgaaaatata gatagtgcaa
     2221 gtgaagaagt tttatcaatg gtaaaaggtt ttgaaccaca acttttcata gctggtcctg
     2281 catttaatgc tggtagatat ggggttgctg ctggaacaat aactaaagtt gtaaaagatg
     2341 ctttgaatat accagcttta acaggtatgt atgttgaaaa tccaggagca gatatgttca
     2401 aaaaggatgt ttatgtagta gaaacttctg attctgcagc aggtatgaga aaggcacttc
     2461 ctaagatagc taaacttgca gtaaaacttg ctaatggaga agaaattgga actcctaaag
     2521 atgaaggata catagctaga ggaataagag ttaactactt ccatgaagac agaggttcta
     2581 agagagctgt tgacatgtta gttaagaaaa taaaaggtga accatttgaa actgaatacc
     2641 caatgccaaa ctttgataga gttgacccaa gtaaagctgt aaaagattta tcaaaatgta
     2701 aaatagcttt agttacttct ggtggtatag ttccaaaagg aaatccagat agaatagagt
     2761 cttcttcagc ttctaaatat ggaacttatt ctatagcagg agttatggat ttaacagaag
     2821 agacttatga aactgcacat ggtggatatg acccagtgta tgcaaattta gatgcagata
     2881 gagtattacc tgttgatgtt cttagagatt tagaaaaaga aggtgtaatt ggaaaattac
     2941 atgagacttt ctatacaact gtaggtaatg gtacatctgt tgcaaactct aaaaaatatg
     3001 cttctgaaat tggagcagca ttagtggctg atggtgtaga tgcagtaata ttaacttcta
     3061 catgaggtac ttgtactcgt tgcggtgcaa cgatggtaaa agaaatagaa aaaactggac
     3121 ttccagtagt tcatatgtgt acagtagtac ctatttcatt aacagtaggg gctaatagaa
     3181 tagttccaac tatagcaata cctcatccac ttggaaatcc agcgcttgac cctacagaag
     3241 aaaaagccct aagaagaggc cttgtagaaa aagcattaaa tgctttaact acagaagtag
     3301 atggtcaaac agtatttgaa aaataaaaat aacacacaca tatattatat agataaaaat
     3361 ataaacacac actaaaaata aaaaataact atacacaaaa aaataatata gatttcatga
     3421 atggataaat tggagttatt cattcatgaa atcttaaaaa atgccaaaat acataaacaa
     3481 taatagaatc aaatatacaa taaaatacaa tgattagagc ttttaataag tttaataaga
     3541 ttaaaaaata agtgttcata aaatttatgg aggtgcacaa aatgacttat ccagttttaa
     3601 aaggggcagg ctatgtactt attcatactc cagatatgat agtgcaaaat ggaagtactt
     3661 gtacagttga aagagcaaca aatccagatt cagaattttt aaaagaagta agtaatcata
     3721 taagaagtta tgaagatgta gttaactaca tgccaaatca agtttatata ggaaatagaa
     3781 gaccagaaga attaagagat ttaccaatgc catggtgtga acaaaagatt gagggaacta
     3841 gaaatggtaa gtttggagaa ataatgcctc aagatgagtt catagcttta atgcaaataa
     3901 gtgatgcatt tgatttagta aaattatcac aagaatttat tgatgaagta aagccaaaaa
     3961 tcgaaaataa ctaccctgaa attgcaccat ttgtaggtaa attaaagggt gatgatatag
     4021 aagaaggtaa agaattagta gcaacacaca tagctgaagg attataccat gatggaaaat
     4081 ttgttggtta tgtaaaaaga gcacatgatg tagatgttaa cttaaatgcc catacaatgt
     4141 ttgaaaatct agtagtaaaa gcatctggag ttttatctgc tattcaaatg ctaagacata
     4201 gcaaaataga ccctgctgaa atagattatg ttattgagtg ttcagaagaa gcttgtggag
     4261 atataaacca aagaggtgga ggaaactttg ctaaatcaat agctgagata gcaggacttc
     4321 aaaatgcaac tggttctgac acaagaggat tttgtgcagc accaactcat gcattaattc
     4381 aagcagcagc tttagtaaaa gctggcatac ataaaaatgt aatggtagta gcaggtggag
     4441 caagtgctaa acttggaatg aatgctaaag accatgttaa aaaaggtctt cctgtacttg
     4501 aagatgttgt tggaggattt gcagtacttg tatctgaaaa tgatggtgta aatccagtta
     4561 taagaactga tttaacaggt aagcatactg ttggaactgg ttcttcacca caagcagtta
     4621 tgacagcact tataacttca ggtcttgata gagctaattt aaaaataaca gatgttgatg
     4681 tgtattcagt tgaaatgcaa aacccagata taactaagcc agcaggagca ggagatgttc
     4741 cagaagcaaa ctacaaaatg ataggggcac ttgctgttaa acgtggagac ttagaaaaga
     4801 aagaattgaa agattttgtt tctaacaaag gattaccagg atgggcacca actcaaggac
     4861 atataccatc aggagctcct tatataggat ttttaataga tgatttaact acaggaaata
     4921 gaaatagagc tatgatagtt ggaaaaggta gtttattctt aggaagaatg actaacttat
     4981 ttgatggagt atcttttata gctgaaagaa atacaggtgt tactgaagag acttcaggaa
     5041 tatctaagga tgaaattaag aagataatag ctgaatctat gaaaaaacta gcattagata
     5101 tgttagaaga ataggggtga ggatatgtca aaaaaagtaa tcgcagatgt attcttagaa
     5161 gtagctaatg caatagaaag cggcgaattt ggcaaaaaag taaaaattgg tgttactaca
     5221 cttggaagtg aacatggtgt agaaaacatg gttaatggtg cacaattagc aaaatctaat
     5281 ttatttgata ttgtgcttat tggaccaaaa gtggaaactg accttgaagt agttgaagtt
     5341 aatgatgaaa aagaaatgca tgcaaaaatg gaagaattat tagattcagg atatattgat
     5401 gcttgtgtta ctatgcatta taattttcca ataggtgtat ctacagtagg tagagtaata
     5461 actccagcaa aaggaaaaga aatgatactt gcaacaacaa caggaacaag tgctactaat
     5521 agaatagagg caatggtaag aaatgccatt tatggtattg ctacagctaa atcaatgggt
     5581 aacaaatgcc caaaagttgg aatacttaat gttgatggag caagacaagt tgagaagtgc
     5641 ttaaaagaat taaaagataa tggatatgac atggagtttg cggattcaat tagagcagat
     5701 ggtggatgtg taatgagagg taatgattta cttgtaggtg cacctgatgt tatggttact
     5761 gatacattat ctggaaatat atttatgaaa gtattttctt catatactac aggtggagat
     5821 tatgaagctc aaggctttgg atacggacct ggagttggag aggactatga tagaaaggta
     5881 cttatagtat caagagcatc aggttctccg gtagtagcaa atgctttaaa atacgcttat
     5941 gatgttgtaa agggagatat aagtaatgtt gcaagaaatg aatttgcaaa ggttaaaaaa
     6001 gcaaaatttg atgacattat atcctctctt actaaaaaag aagttaaagc tgaaaaagta
     6061 gaagttaaaa tgccagataa agaaattgtt actcgtcaaa tagctggtgt tgatataatg
     6121 gatttagaag atgcagtatc tgagttatgg aaaaacggaa tctatgctga aagtggtatg
     6181 ggttgtacag gtccaatagt tttagtaaat gaagctaaag gagacttagc agttgaaact
     6241 ttagttaaag ctggatatac tgcaaaataa gtacattttt tatatgtaaa taagaacata
     6301 tagttataat tttattgatt ataattatat gttcttttta ttttatatct tgtcaatatt
     6361 tatatattaa cacaatccat ttatttgatt ctctagctaa tttattatat ttattcaatt
     6421 aaaattaagc tttctaacta aataaaaact aatgaagtat taaaatgaaa ttgataaaat
     6481 atgaaaaaat tttaggaata aagtatctgt tagataaaaa taagattaat tagtgatata
     6541 atataattaa gataaaattt attatttgga ggtaaaatca tgagcagagt aaaaaatgta
     6601 gtagaattgt tggaaactaa aggtgttgat gcactatatc taacaaagaa aactaatgta
     6661 aactatataa gtggttttcc tgatgaagaa gcatatgcag tgatttgcaa agatggaaac
     6721 tttttagtta ctgatagtag atatatggag cttgcagaaa aggtttgcaa agattttgaa
     6781 attataaatt ggcataattt tgatagaagt gtggcaaagg cagttaaaag tgtatgtgat
     6841 aaagtgggta taaagaaact tggatttgaa agaactaata tagtgtttga taaatatgaa
     6901 gaacttaaaa atctgattga gaaggataat ggagaattaa tacctacaga aaatattgta
     6961 gagactttga gatatgtaaa agacaaagat gaaatcaaaa atacaagaaa agcttgtgaa
     7021 atagcggata aggcattaga agaattgatt ccacatataa aagctggagt tagcgaaatt
     7081 gaactggcta caaggttaga gtactttatg aaaatgaacg gagcacaaaa tatagggttt
     7141 gaaactatac tgatatcagg agcaaagact tcattgcttc atggtaagcc tagtgataag
     7201 attatagaaa aaggtgattt tgtacttata gactatggtg ctatgtataa tggctatata
     7261 tctgatacaa ctagaacttt tatagttgga ggagcttctg aaaagcaatt agaaatatat
     7321 aatttagtta aggaagctca aaatgtaggg gttgagaaca tgaaagctgg tgtacatgcg
     7381 acaattccag atgctgagat aagaaaagtt gtcaaaaaat atgaagacta ttactatcaa
     7441 ggtataggac atggagtcgg tagagatgtt catgaagaac ctttcattgg taattatggt
     7501 gataaaatca tagaagaggg atgtataata acaatggaac caggtattta tttccctgga
     7561 tggggtggag ttagaataga agatactgtt ttgattacta aaaatggtcc agaaagactt
     7621 actaaattcc ctaaagattt gatgatttta gacaaataat ttaatttata tatatttttt
     7681 tacataaaat ataacataat ctcactatta agctctattt acatattata gtaaagaggg
     7741 cttaatttta ttgtgagagg tgatagtata tatgtataaa aaacataaat ttataaaaaa
     7801 tataaaaaaa gcctggtata aaatcaaatt aattgataga tgccttataa ttgtaatggc
     7861 aatattaatg tttcaatcta tatataactt atttgttaat gaagtaaatt cacaagatac
     7921 tacaactatt gatgtagtag taagaaccac ttcagcagct atatttgggt attttttaag
     7981 tgctaatttt ataaagagac ctttaaggaa gtatacaaat actgatgtat cttcaaatct
     8041 ttttatagat aataatttat ccccaaaagg tcaagagtct aatcaaaata atataatgaa
     8101 tgtcaaaaat accattggat ttacttcaga agctgataat tatgaaaaga aaatacctat
     8161 taataataat gaaggttttg aagaaggaga aactagtgaa ttgcaaatta ttatagctac
     8221 tgtaatttgc atagtagcat taacagtact ttttgttgta agaaatttta caactacaac
     8281 atcagcttct ttaggtacta tttcacagat gagagatttt gtctcaggat gcgtgggttt
     8341 tttgctaggt tgtccaagta aacctgatta aaaaagtatt atcaataaaa ggtattttaa
     8401 aaagtatcag tataataata tttagattta aatgtataca attataattc tgtaacattc
     8461 tatattgtat ttttatactg atactctaaa ctatactatt tattaaaata ttttaacatt
     8521 gtatttaaac aaaatattat atctgtataa tataaattta gtttatatga atagatgcca
     8581 tatactcaat aaaaagtttc atactatgag atatgtattg atttcttctc caaagaaccc
     8641 caatatttgc agtaattgca cgttctaatg gtattgaaca gagattttct gtattttgaa
     8701 atatttcttt atacaatatt gttgaagcca agtcattttc tagtaaataa cgtatggtag
     8761 aaagttgatt tgagtgtaaa attatatttg gcttagcttt ttcaagtctt tttgtaataa
     8821 tcttatagtg ataagaacct tcagataaga ttacaaaagg tacatttttt aacatataat
     8881 tcgatataat aggatgtttt gccaattcat ttttgggatt tgtaactaag tataattctg
     8941 ttttatagat tgtctttgaa tctatatcat cataaatatc actgtcaatt ataccaatac
     9001 aaaaatcaag tgtagattct tcaatcattt tagctccaac aaatgttgga acttcaaaaa
     9061 ttttaagctc tatatctgga taaattacat taaacctagg aagtattttt gaaagcaaaa
     9121 acgtacctaa aactgtagga atacctattt tgagagaagc ttttctcttt tttcctaaat
     9181 caagtgcgtc acattgaaaa tcctcaaatt gttttataaa atgttttgac ttgtctaaaa
     9241 aaatttttcc ttcaggcgtt aactctattt tattacctat tctgtaaaaa agttcaaagc
     9301 caagttcttt ttctaattct ttaattgcta atgttatgga tggttgagaa atatgaatac
     9361 tttccgcagc cttagtcatg tttttatatt tacagacagt ttgaaaatag tataaatagt
     9421 ttatattcat ttggtattag ttcctttcat ttacatatta gtgacaagta tataattaac
     9481 aatagcataa gttttgtata ttgttgtaaa aaaatagaat agttttaaac tatcatagca
     9541 taagtttttg atattttcaa tctattaaga atagtgctat tattaaatta acaaaagaga
     9601 atagctggta tggtttagag taaatcaatc tatattacag ttatatcaac gaatatcaaa
     9661 aaattatcta ttaaaaaata aaaattattt ttacaattaa attgttaatt aaatcataaa
     9721 gaaaaaagtg atattaacaa gataaaattt ttttaatcag aaatgttaat ttaataacaa
     9781 aatagagtat tggggagaaa ggagaaatat attttttaat aatatttgtt aatttaataa
     9841 cgaaaaatga aagggagtga tttagtgaag tttgaattga tggatttttt aatgaatcct
     9901 tttgtcctta tgtttgctgc agtaattaca ggaattttat ttggaaaaat taaatttggg
     9961 aagtttaatt ttggggtatc aggagcatta tttacaggat tatttattgg atggcttgca
    10021 tatagcttgg ggaatctaat aatagcaaaa ggggaaacag cagcaggata taaggcagca
    10081 actgtaatga tggggaatgg aattatttct tctgacttct ttgatttctt tttaataata
    10141 tttgttgcag cagttggtct attggcagca aaagatatga aagctgtatt aaagaagtat
    10201 ggagcaagat ttgtaatttt aggagttctt ataactttta taggtggctc tatgacttat
    10261 gcaatgactt tactatcaag tgataaagga agtagtgctt atgaagtttc tggcgtttac
    10321 acaggagcat taacaagttc gcctggatta gctgctgctt tagaaacagc tggtaaacat
    10381 gcagaagatg tatcaaaaga atttgaaaaa gcttcaataa aagataaaaa agaaatttta
    10441 aaagtagtcg acccagaagg caaattggat gtgaatacaa ctacatcact gactcaagag
    10501 caaatagata agtatattgc atatgctgaa gctggtgttg gtataggtca tgcagtcgca
    10561 tatccttttg gagttttaat agtaatctta ggtgtaaatt tccttccaaa attatttagg
    10621 atggacctaa aagaagaaag aagaaaatat gagaaggaaa tgaaggaagc aagagatagt
    10681 gtttctggta aaacgatacc agaagttcca tttaatataa taacattttt cttgacatgt
    10741 ttagctggat acttagttgg aggaatacat gtatttatgg gaccactagg gtacttcaca
    10801 ctaggtgcaa caggaggttc actaattgta tctctagttc ttggatatat aggtaagata
    10861 ggggtagtaa atttcagaat ggaagaaaag gttttaaata ttttaaaaca aatagggcta
    10921 gtgtttttct tagcaatagt tggattaaga tatggtggaa aagtagttga ttcaattatg
    10981 acatctggta tgcacctagc cttagtagca atagcagtag gtgttacagc tatgatgata
    11041 ggattcttag ttggcaaata cgtatttaaa ttaaattgga ttttattatc tggagctgtc
    11101 tgtggtggta tgacatcaac accaggtctt ggagctgctg ttgatgctct tgatagtgat
    11161 gacccagcag caggttatgg tgctacgtat ccatttgcat tattaactaa agttatattg
    11221 gttatagttt tacacaaact tccaatgtaa ataagatttt atatgggggg atatttatga
    11281 ataatgaagg agcatttgaa gaattaagtg gaagattaag aaataaagaa ttgtgtgatt
    11341 taataatgaa tgcagatgaa gctgctaaaa ttatagagga tggcatggtt attggagtta
    11401 gtggatttac accttcaggc tatccaaaag cagtaccatt agcagtttca gaaagagcta
    11461 aaagtggaga aaagataaaa ttaaccgtat attcaggagc atctttaggt ccagaagttg
    11521 atggagcatg gtcagaagca ggtataattg aaagaagact tccttatcaa actaattcaa
    11581 tacttagaaa taatattaat aaaggtgttg ttgattatat agatatgcac ctaagtcatt
    11641 ctactcaatt tttaaattat ggaactatac ctaaagtaga tgtagctata gtagaagcac
    11701 ttgcaataac tgaagaaggg aatattatac caactagtgg gataggtaat tctccatctt
    11761 ttataaaaag tgcagataaa gtaatagtag agataaatct tgcaaaacca atggaaatgg
    11821 aaggtatggc agatatatat attacggaaa atccaccaaa tagaaaacca atagaaataa
    11881 atcatcctaa ggatagaatt ggaacaacat atataccttg tggtttggat aaaattgcag
    11941 gagtagtaat taccaatatg caagataaga cccgtccatt gggggttgta gatgaagctt
    12001 cgaaaaaaat atcaaataat attattgctt ttttaagaga agaagtaaag tcaggcagat
    12061 tatcaaagaa tttacttcct cttcaatctg gtgtaggaag tgtagcaaat gcagtattat
    12121 atggattatg tgaatctgaa tttgaaaatt taacttgtta tacagaggtt gtacaggact
    12181 ctatgttgga tttaataaga atgggtaaag ttacaatggc atctactaca tctgtaagtc
    12241 catctccaga aggattaata aaatttgaaa aagatattga tttttttaag gataagatta
    12301 tattaagacc tcaagaaatt agtaacaatc cagaaatagc cagaagaatt ggtgtaattg
    12361 caatgaacac tgcaattgaa gttgatattt atggaaatgt aaattctact cacataatgg
    12421 ggtcaaagat gatgaatgga attggtggtt ctggtgattt tgctagaaat ggagcaataa
    12481 ctatatttag tactgagtca attgcaaaaa atggtgatat atcatcaata gtacctatgg
    12541 tatctcacgt agaccacact gaacatgatg taatggtaat tgtaacagaa caaggatatg
    12601 ctgatttaag aggtcttgca cctagagaaa gagctataaa aataatagaa aattgtgccc
    12661 atccagatta taaagaacaa cttagggatt atttaaatag agcttgtaaa agtggagcaa
    12721 aacaaacacc tcacatattg gacgaagctt tatcatggca tagtaaattt atgagtacag
    12781 gtacaatgaa aaaggctgaa acttttaaaa gtgcattata aaatatttaa taaattggag
    12841 gtagatttta aatggaaaaa gcagtggaaa actttgaaga tttaagtaag gaatacatta
    12901 atggttatat tgagagagca agaaaagctc aaagagagtt tgaatgttat actcaagaac
    12961 aagtagataa aatagtaaaa atagttggaa aagtagtgta ttataatgct gaatatttag
    13021 ctaaacttgc tgtagaagag actggaatgg gagtatatga agataaggta gctaaaaata
    13081 aaagcaaagc aaaagttata tataacaatt taaaagataa aaaatcagtt ggtataattg
    13141 atatagatag agaaactgga ataacaaagg tagctaaacc agttggagta gtagcggcaa
    13201 taactccttg tacaaatcca atcgttacac ctatgagcaa tgcaatgttt gcacttaagg
    13261 gaagaaatgc aattattatc acacctcatc ataaagcaat tggatgtagt acaaaaactg
    13321 tagagatgat taatgaagaa ttagaaaaaa taggagctcc agaaaattta atacaaatac
    13381 ttgaccaaca atcaagagaa aatacaagaa atttaatttc atcagcagat gtagtaattg
    13441 ccactggagg aatgggaatg gtaaaagctg cgtactcaag tggaaaacca gcacttggtg
    13501 taggggcagg aaatgttcaa tgtatcatag atagagatgt agacattaaa gaagcagtac
    13561 ctaaaatcat agcaggaaga atttttgata atggtatcat ttgttcaggt gaacagtcag
    13621 taatagtagc agaagaaatg tttgataaaa taatggatga atttaaaaat aataaaggtt
    13681 ttatagttag agataaagta caaaaagaag ctttcagaaa tgctatgttt gtaaataaat
    13741 ctatgaataa agatgcagta ggtcaatcag ttcatacaat tgcaaaaatt gcaggtgttg
    13801 agataccaga agatacaaaa ataatagtaa tagaagctga tggaccagga gaagaggata
    13861 ttattgcaaa agaaaaaatg tgtccagtta tatctgcata caagtataaa agttttgaag
    13921 aaggtgtagc tattgcaaaa gcaaatttaa atgtagaagg aaaaggacat agtgtatcta
    13981 tacattcaaa tacagttaaa aatatagaat atgcaggaga aaatatagaa gtatcaagat
    14041 ttgttattaa tcaatgttgt gctactagtg ctggaggaag tttctttaat ggtcttgcac
    14101 ctactaatac tctaggttgt ggttcttggg gaaataacag catctctgaa aatcttgatt
    14161 ataagcatct aattaatatt tctagaattg cttattatat gccagaaaat gaagtcccta
    14221 cagatgaaga actttgggga taaaatttag acaaaaaata ttcattctaa attaaagtag
    14281 gaatatattt taatttagaa tgaatttaag acataaatta aatttaaatt agtttattgg
    14341 actaaattta aaatattaag agacatatag aagaaggaag tattataatt taaaataaat
    14401 aaaaaaatct ataaaagaga aggagtggtt ttattatggc attaatgaca ggagctcaat
    14461 atattgaaag tttgagaaaa ttaaatacta aggtttatat gtttggtgaa gaggttaaga
    14521 attgggttga ccatcctatg attagaccat ctataaattg tgtagcagca acttatgact
    14581 tagctcatga cccagaatat gcagatttaa tgacagttac ttcaaatatc acaggtgaaa
    14641 aaataaatag atttggtcat cttcatcaaa gtgttgatga tttgataaaa aaagtaaaaa
    14701 tgcaaagact atgtggtcaa aaaacagctt cttgtttcca aagatgtgtt ggtatggatg
    14761 cgtttaatgc agtgtattca acaacatttg agtgtgataa agcccatgga actaattatc
    14821 atgataattt tgtaaaatac ttaacttata tacaagaaaa cgatttagta gttgatggag
    14881 ctatgactga ccctaaggga gatagaagtc tatcacctag tgctcaacct gacccagata
    14941 tgttccttca tatagtagaa agaagagaag atgggataat tgtaagagga gcaaaggcac
    15001 atcaaactgg ttcaataaat tcacatgagc accttataat gcctactata agtatgactg
    15061 aagcagataa agactacgct gtatcatttg cagttccttc agatgctgaa ggagtgttta
    15121 tgatttatgg aagacaatct tgtgacacta gaaaattaga agaaggtgca gatgttgatt
    15181 taggaaataa ggagtttgga ggtcaagaag ctttagtagt atttgataat gtatttgttc
    15241 caaatgatag aattttctta tgtggagaat gggatttctc tggtatgtta gtagagagat
    15301 ttgcaggata ccatagacaa agttatggtg gatgtaaagt aggagtaggt gatgtaataa
    15361 taggagcagc agctttagca gctgattata atggagcaaa caaagcttca catataaaag
    15421 ataaactaat agaaatgact caccttaatg aaagcttata ttgctgtggt atagcatgtt
    15481 catcagaagg tcacaaaaca gaagctggaa actatcaaat agatttactt cttgcaaatg
    15541 tgtgtaaaca aaatgtaact agatttcctt atgaaatagt aagacttgca gaagatatag
    15601 ctggagggct tatggttact atgccttcag aaaaagactt taaatcagac cttaaagttg
    15661 gaacaagtgg aatgactatt ggcgaagtat gtaacaagta ttttaaagct tcttcagtgg
    15721 cttcaacaga agaaagaatg agaatattaa gattcttaga aaatatatgc cttggttcat
    15781 ctgctgttgg ttatagaact gagtcaatgc atggggcagg ttcaccacaa gctcaaagaa
    15841 taatgatttc aagacaagga aacataaatc aaaagaaaga attagctaag aaaattgcgg
    15901 gaataaaaaa agaggaagct ttaaatttat aaaaagttga tatataaagg gaaattatat
    15961 attaatgtgt attaattaat gaaaaaatat gtagtataag agatagttat aaaattatgg
    16021 tggtttttag ctaagtaaag aaatttttaa taaggagaat ttacacatga cctgtggagt
    16081 tagattttgt gggggctgta accctagata cgaaagaggg aaggctctta caataatcaa
    16141 agagcacttc aatggtaggg tagattttag tatagcacaa gagaacgtcg agtatgattt
    16201 gctacttata attggaggat gcgcaactct atgcgcatcc tatcaacaat ataaagtgaa
    16261 aaatgaatgt gttaagatgt gggattttag ccatatagaa agagttataa atgtgattga
    16321 atcagcaatt tgtgaaactt aaaaaagcta aatgggggga tttcattgag ttggcaagaa
    16381 ttgtatcaaa gtaaattatg tagtgctaca gaagctgtaa aacagattaa aaacggtgat
    16441 actgtagtat ttgcacattg tgtaggtgaa cctcctgcac tagtagaggc tatgattgaa
    16501 aatgctgaac aatataaaga tgttgagatt aaacatatgg taagcttggg tagtggtgga
    16561 tatactgcta agggaatgga agctcatttt agagtaaatc caatgtttgt aagtggaaat
    16621 gtaagaaagg caattgaaaa tggagatggt gattttacac ctgcattctt ccatgaagta
    16681 ccaaagttgc ttagagaaaa aagattaaaa tgtgatgttg ttttagcaca ggtaactcca
    16741 ccagatgaac atgggtattg tagcttagga acatcagttg attatactta tgaagccatt
    16801 aaaactgcta gaacagtaat tgttcaagtg aatgaccagt ttcctagaac ctatggtgag
    16861 gtagtccatg tatcagagtt tgactatata gtggaaaaat cacaaccttt atttgaacta
    16921 caacctgcaa agattggaga agtagaagaa gcaattggta aaaattgtgc ttcattaatt
    16981 gaagatggtt ctactttaca gctagggatt ggtgggatac cagatgcagt tatgttattt
    17041 ttaactgata aaaaggattt agggattcat tcagaaatga tttcagatgg gactttggcc
    17101 ctttatgaaa aaggtgttat aaatggtaaa tataaaaatt ttgataaaga aaaaatgaca
    17161 gttaccttct taatgggtac taaaaaatta tatgactttg caaataataa ccctgcagta
    17221 gaggtaaaac cagtagacta tgtgaatcat ccagcaatta taatgaaaca acataagatg
    17281 gtttctataa attctgctat acaagttgat ttaatgggac aggtagttgc agaggctatg
    17341 ggacttagac aatttagtgg tgttggaggt caagttgact ttatacgtgg agtgtctatg
    17401 ggtgaagatg gaaaggcaat tatagctatg ccttcaataa ctacgaaaaa agatggtaca
    17461 gtaatttcaa aaatcgtgtc tattgtggat gaaggtgcac ctataacgac atcaagaaat
    17521 gatgttgact atattgtaac agaatacggt atagcagaat taaaaggaaa atctcttaga
    17581 gaaagagcga gaaatttaat aaatatagct catccaagtg taagagaaag tctagcagta
    17641 gaatttgaaa agagatttaa ggagaaatat taataataga ggggtgttaa taatggaagc
    17701 acttagagta gttccaaaga tattttactt tgatacattt aaagaattta atgaagaatt
    17761 taaaatagga aaaaatgatt tagtaattac taatgagttt atatatgagc catatatgaa
    17821 acctcttgga atagatacaa atttaatttt tcaagaaaag tttggaacag gtgaaccatc
    17881 tgatgaaatg atagactcca tgacaaagga aatgaagaaa tacaattttg atagaattat
    17941 tgcatttgga ggaggaacaa tagttgatat atgtaagata ttagcacttg atgttccaga
    18001 aaagtctatt gatttatttg aaggagaagt atcacctaaa aaagtaaaag agttagtagt
    18061 agttcctaca acatgtggta caggtagtga agtaacaaat gtagctattg cagagttaaa
    18121 atctaaacat actaaaaagg ggcttgcagt agaagaaaca tatgcagact atgcagtgtt
    18181 aattccagag acagtaaaag ggttacctta caaattcttt gttacaagtt cggtagatgc
    18241 tcttatacat gctattgagt cttatctttc tccaaaagca agtccattta cagagatgta
    18301 ttctctacaa gcaataaaaa tgattatgga tgggtataag aagatagttg ataaaggaga
    18361 agaagagaga tttaatcatc ttagagactt tgtacttgca tcaaattatg caggaattgc
    18421 ttttggaaat gctggttgtg cagcagttca tgcactatct tattcaatag gtggagcttt
    18481 ccatgttgca catggtgaag caaattatca gttctttact gaagtattta agatgtattc
    18541 tagaaaaaaa cctaatggaa aaattataaa atgtacaaaa attttagcag atgcccttga
    18601 atgtgaccct aactgtgatg tttatggaga attagaaaag tttttaaata aattaatagc
    18661 taaaaaagct ttgagagaat atggaatggt ggaaagtcag atagatgaat ttacagatag
    18721 tacaatagca aatcaacaac gactacttgc taataattat gtagagctta gtagagagga
    18781 aataagagag atatttgcaa atctttatta atacaatttt ttatttatat gtatagtcaa
    18841 cataaacaat gctaattata tttatgtaca aataaaaata agggaactta aattttaatt
    18901 aaagttaagt tctcttattt ttatatggag ttaaatataa gtatattttt aatttaaaat
    18961 atgcttatat ttaagtctat tactttattt gaaacaattt gtagaatttc aaattccttg
    19021 acagtaataa gtaagtaatt gtataatcaa actgcaatat attaataata ttagtgaaaa
    19081 tgaaaggatt ataaaatatg tataaatcag aaaatttttc aaaaaaatct gtattaataa
    19141 attcattttt aagtaaattt ttaaaaataa ttggatgggg tttggtaagc ttatttacat
    19201 tatcagcctt agtactttca actatttcca ttataattgg atgggctgta atacttccta
    19261 ttcaagtatg tgcagtattt ttacttatag gaggagtttg tatttttaaa ggtaagagat
    19321 taggagacca aatttctcgt tataaaaaat attgtacaat tataaacaat agaaatatta
    19381 ttccagtaga acttattgca gaaaacacat ctaaatcatt aaattttata ataaaagatg
    19441 tacaaaagat gatagacaaa aactacttta taaatactta tattgataaa agaaataatc
    19501 agattgtgtt aacaaatgaa gattttgttc caccaagata tgaagatgta gcatatgaag
    19561 tcaaagaaga agaaaagact gatgaatcca atgaagttga tacaattata aataaaggaa
    19621 tgaattatct aacacaaata aaagaagcta ataaaaatat taaatcagaa tcaatgggcg
    19681 ataaaattgt acaagtagaa gatgttacaa gtaaaatatt tgatgttgta aaacatgacc
    19741 catcaaaact tactcaaata caaaaattta tggattatta tcttccaacc actttaaaac
    19801 tattaaattc atatcataca ttggaagaac aaggaattga tagagaaaat ataacaacaa
    19861 ctatggaaag tattgaaaat acaatgaata cgattgtagt agcttttgaa aatcaattgg
    19921 actatctgtt tgaagatgaa gccattgata tttctacaga tattactgtt ttagagaata
    19981 tgttagtcca agaaggatta acgagtggta aattttaact tcaatagaaa aaatattctg
    20041 ttatttttaa acaattaatt agtcataaag tataaaatgt gtattatata agttaatcag
    20101 tactttatga aggaagagga gagaaagata caatgaataa tttagatgat ataccagtta
    20161 tgccaacttt gacacttgac ccatttggag aagaatcaaa cactaatgat atagataatt
    20221 ctgacttatt aatgaaaaag gatgaaaaag acccagaaga agaaaaactg tctgaatctg
    20281 aacgcaagat ggtaaaggaa tttgcggaaa aaatagatat tacaaataca aatatgattt
    20341 tgcaatatgg agctggtgct caaaaaaagg tagcaagttt ttctgaaaca gctttaaaga
    20401 gtgtaaagac aagagattta ggagaagttg gagatatgct tacaaatcta gttactgatt
    20461 taaaagcatt ttcagcagat gaaaaagaac aaagtggttt tttaggaatt tttaaaaagg
    20521 caaataataa aatttcaaat ttaaaaacta agtatgatag tgcagaggta aatgtggata
    20581 aagtaagcaa agagcttcaa aagcatcagg ttaaaatatt gaaagatata gcaatgcttg
    20641 ataagatgta tgaattgaac ttagcttact caaaagagct tactatgtat attatagcag
    20701 gaaaacaaaa attaaaggat atgaaagaat atgagatgcc aaagttgaga gaaaaagctc
    20761 gtttatctgg ttcaacagaa gatgctcaat ctgtaaatga tatggtttca ctttgtgaca
    20821 gatttgaaaa gaagattcat gacttagaat taactagaat ggtatcgctt caaatggctc
    20881 ctcagataag attggttcaa aatagtaata atctgatggc agaaaagatt caatcaacta
    20941 tagtaaatac cattccatta tggaaaaatc aaatagtgct tgctcttgga atttctcact
    21001 taaatcaagc tatgaaagca cagcgtgaag ttagtaatat gacaaatgag cttttattaa
    21061 agaatgcaga aactttgaag atgggaacaa tagaaacagc aaaagaatca gaaagaggaa
    21121 ttgtagatat agacacaatt aagaaaacta atcaatctct aatatctact attgatgagg
    21181 tagttaagat acaacatgat ggtagaataa agagacaaga agcagaagtt gaattatcaa
    21241 aaatagaaaa tgaacttaaa agcaagcttc ttgaatttac agttaaataa aaattatact
    21301 aagataaaaa gagactgtct caaaattgga tatgtccctg tcaagtagac agacttaaaa
    21361 aaggctatct ttactcggaa tgagttcgat attcaatcgg actcattccg ttttttaatt
    21421 gtattctttc attattataa aaataaatat aatcatttat tagactaatc atttcctttg
    21481 gatctgtagt atcaattaaa tatattaatt cagatttaaa atgactgaaa aaactttcta
    21541 taggagcatt atcccagcag ttgccccttc gggacattga ttgtataata ccattatcct
    21601 taagcctttt agagtaagat ctacttgtat attgtgaacc ttgatctgta tgtaaaatac
    21661 aatctttagc taaattcata tttattagtt gatttactgt attattaact aatttagtat
    21721 ccatttttgt acttatctca tatgccacta tttcaccatt aaataaatct ttggctacat
    21781 tcatatataa gaatttttta ccgataggta tgtatgttat atccatacaa attttttcta
    21841 atggttgatt agctctaaat tcacgattta atatattatc atatatctca tatgatttaa
    21901 acttacgtct atatactttc tttctaataa tagatttaat acctagttct ttcatcaacc
    21961 tatatactct cttatgatta ataggggtat caagtatttt atttaaataa gtacaaattc
    22021 tttttctacc ataagttcct ctatattttt tatgtatttc aattatataa tccttaataa
    22081 ttctatcttt gataccccta tcggaagatt ttttagaata acttagccat ttatagtaac
    22141 cgcttcttga cacacttatc aacttacaca tactagaaat actatacttc ctagacagaa
    22201 gtttaattgt tttaaattta tcgcttactt tgacttgtcc aatagggtac ttaacttttt
    22261 taagaattca ttctccatgc gtaagtactt caattcttct tcaacagatt taaattttct
    22321 attatcattt tctagactaa tatttttaaa tctgccccta gtttcatcat caaatgcagt
    22381 tttgccttta tttttataat cttttaccca gttttgaact tgtgttttgc ttcttatatt
    22441 cagcatctca gcaattttgt atgatgagta tccttctttt atatataagt ttacagcttt
    22501 tagcttaagt tctttgctat attttttgtg ttttttagac atagaaaaac ccccttagag
    22561 tatacgcttt ttagacctat tataatagcc ttttatttaa atgtctacta taaggggatt
    22621 atactaaaat agagataagt ctctttttca ttgtttaaat gattaaagta tatttatatt
    22681 ataaatttct attatctttt aacataacat catgagctcc accttcaact atactagtag
    22741 aagatacaag tgtaagttta gcattttttt gaagttcagg aatgctaagt gctccacagt
    22801 tacacatagt agaacgaact ttacttaaag aaagagttac attgtctttc aaagaaccag
    22861 cataaggaac ataagagtca acaccttctt caaaagaaag tttcttatct ccaccaaggt
    22921 catatctttg ccagtttcta gcacgagcag aaccttctcc ccaatactct ttcatataac
    22981 taccatttat attaactttg ttagttggac tctcatcaaa acgagagaag tatctaccaa
    23041 gcattataaa atctgcaccc atagctaaag ctaaagttat atgatggtca taaacgatac
    23101 caccatcaga acaaattgga atataaatac ctgttttttc aaagtattcg tccctagctt
    23161 gagcaacttc aattatagaa gtagcttgtc cacgtcctat acctttttgt tcacgagtta
    23221 tacatataga accaccacca ataccaactt taacgaagtc agcaccagct tcagctaggt
    23281 aaaggaatcc ctcacggtca actacatttc cagctcctac cttaactgta tcaccatatt
    23341 tttcacgaat gaaatcaagt gttatttttt gccactcaga aaaaccttct gaagaatcta
    23401 tacataaaac atcagcacca gcttcaacta aagcaggaac acgttcagca aagtctctgg
    23461 tattaatacc agctccaact acatatctct tagaagaatc taaaagttca agtggatttt
    23521 ctttatgaga agagtaatct ttacggaata ccatatacat aagattacca ttgtcatcaa
    23581 gtacaggaag agaattaagt ttgtggtccc atataatgtt gtttgcttcc tttaaagtaa
    23641 catctttgtt agcataaact atagatgaca taggagtcat aaattcacta actttagtat
    23701 ctaaatccat acgacttata cgataatcac gacttgcaac aatacctaat aatttaccag
    23761 ttgaagttcc atcttcagta acagctactg tagaatgtcc agttttttct tttaagtcaa
    23821 gtatgtcttt taatgtgtta tcaggtttaa tatttgaatc acttacaaca aaaccagctt
    23881 tgtgggattt aacacgtgct accatagcag cttcatcttc aatagtttga gaaccgtata
    23941 taaatgaaat accaccttct ttagcaagtg caatagccat tttgtcatca gaaacagatt
    24001 gcataatagc agaagtcaaa ggtatattca tataaatatc tgcttcctcg ccctttttaa
    24061 atttagtaac aggtgttttt aaacttacat tagcaggtat acaatcagta gaagagaaac
    24121 ctggtacaag aaggtattca ctgaaagtat gtgatggtgt atcaaaataa aacgccattt
    24181 tttaatcact ccttatattt ttatactttt tataattgat agtattaatt ataacttatt
    24241 ctttaatttt attaaagtat tttgtcaaat attgagattt ttgaaaaatt taaactattt
    24301 taattgaatt tgtttaatat atattgcctt atagggtaaa gttgtatatt aaataatgtt
    24361 tagtgtttaa tagtagctaa tttgaaatta atctttagtt attcatataa ttacatttgt
    24421 atagtttata ttgataatat tgctagagag tgcataaacc tcaaaaatac aga
//
LOCUS       DAMJVI010000052        24317 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.054, whole genome shotgun sequence.
ACCESSION   DAMJVI010000052 DAMJVI010000000
VERSION     DAMJVI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24317
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.054"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            109..300
                     /locus_tag="PC545_002166"
     CDS             109..300
                     /locus_tag="PC545_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDF2456933.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMVSRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="PC545_002167"
     CDS             455..1864
                     /locus_tag="PC545_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2456934.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKKGQVYKKLGYKDILKNLYKM
                     DTTEILGTDNLHFPEGIIKNSQKKASRVFKSDYTCYLVNGSSCGIQSAIMSVCSPKSK
                     IITNRDCHQSVINGCILGDIDIEYVPCEISEETNILKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKKMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPAFTQSSMIHIQGDRVDIDKILNMLRITESSSPSYLLLSSLELAVDIYENKGK
                     ELMEELLNNIITFKNKVDSNINIYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTAEDFSSLETALNNVVKEDFKFEKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKVCGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVDLNFIQVIDLEDI"
     gene            1995..2684
                     /locus_tag="PC545_002168"
     CDS             1995..2684
                     /locus_tag="PC545_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDF2456935.1"
                     /translation="MKGKLIIIESGSDASGKATQTEILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIIIADRY
                     TTSNMVHQASKMDICDREEYLNWLADYEFNLFKIPQPDCVIFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLEKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKPMER"
     gene            2687..3622
                     /locus_tag="PC545_002169"
     CDS             2687..3622
                     /locus_tag="PC545_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDF2456936.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHKDYKIYIINNAEK
                     MTVEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKRYLI
                     NTGIEEERAQLLAIFSRGSIENALNLSQSSEFSMMREDIQQYIQIMLDKNIVEILNIP
                     NNMEKYRGKIIALLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMNGKISYSQLS
                     KIIDIIEDAKSKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3619..4512
                     /locus_tag="PC545_002170"
     CDS             3619..4512
                     /locus_tag="PC545_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HDF2456937.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVYLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4590..5336
                     /locus_tag="PC545_002171"
     CDS             4590..5336
                     /locus_tag="PC545_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDF2456938.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSIKLNGLEERVEIINED
                     IKYIDKVLDINGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     PRGKFFMIHRPTRLVDIITLGRKYKLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNKDIGEL"
     gene            5337..6170
                     /gene="rsmI"
                     /locus_tag="PC545_002172"
     CDS             5337..6170
                     /gene="rsmI"
                     /locus_tag="PC545_002172"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDF2456939.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGDYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHRFIFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMLKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDGLTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6193..6897
                     /locus_tag="PC545_002173"
     CDS             6193..6897
                     /locus_tag="PC545_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDF2456940.1"
                     /translation="MEIGKIIIESLKREDTHKIFIELDNKHELEKIIPKISCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNDKDFFPIHLRKHVSNYLKTYIEEGINKLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGIKLELSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSGEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7085..7513)
                     /locus_tag="PC545_002174"
     CDS             complement(7085..7513)
                     /locus_tag="PC545_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDF2456941.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7550..8980)
                     /locus_tag="PC545_002175"
     CDS             complement(7550..8980)
                     /locus_tag="PC545_002175"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDF2456942.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLEYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGRCKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9112..9696
                     /locus_tag="PC545_002176"
     CDS             9112..9696
                     /locus_tag="PC545_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein A"
                     /protein_id="HDF2456943.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTAVAIITCKFFQRRYR"
     gene            9712..10233
                     /locus_tag="PC545_002177"
     CDS             9712..10233
                     /locus_tag="PC545_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HDF2456944.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10299..10545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10629..12566
                     /gene="metG"
                     /locus_tag="PC545_002178"
     CDS             10629..12566
                     /gene="metG"
                     /locus_tag="PC545_002178"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDF2456945.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTNFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDETKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKIISCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12596..13366
                     /locus_tag="PC545_002179"
     CDS             12596..13366
                     /locus_tag="PC545_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDF2456946.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIETSI
                     TAIELAKKYDFIYSAVGVHPHDVSKLDDTAIETLRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKNPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13703..14134
                     /gene="phnG"
                     /locus_tag="PC545_002180"
     CDS             13703..14134
                     /gene="phnG"
                     /locus_tag="PC545_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDF2456947.1"
                     /translation="MYTVDSTKILINMNLEKLEKIYSMIKDTSSISIIKEPNLATVMV
                     RANESVKNTTFNLGEILVTECSVKVDESLGYGIVSENNNKKAIYLAVIDAVLHSNNSK
                     FDELKNYINKSVYEENLRYEQEIIDEFSLINKTKVQFNAMD"
     gene            14149..14724
                     /gene="phnH"
                     /locus_tag="PC545_002181"
     CDS             14149..14724
                     /gene="phnH"
                     /locus_tag="PC545_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDF2456948.1"
                     /translation="MVNYTQEIYRKMLDSASFPGKLNNIDNINIENNTKLLNGAIGIA
                     ITLLDQEVTFYIENYTQKDIKNIRALTMSNQIDYRKSDYIFLDINSEVDILQIKIGSF
                     EYPDESATIVHQVNDLSTYSQTKYIKLDLSGPGIKSKNSIYINGIREEFIERLSSINK
                     DYPIGIDLILVDKKGEIAFIPRSSKLSWEVL"
     gene            14727..15812
                     /locus_tag="PC545_002182"
     CDS             14727..15812
                     /locus_tag="PC545_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HDF2456949.1"
                     /translation="MAYVAVKGGEEAILQAKNILEFYRVKGDSLPIDVKQIEEQMSYA
                     VDKVMSEGSLYNKELSALAIKQAQGDILEASFYVRALRSTMPRITYSEPINTEEMRIK
                     RRISATFKDIPGGQYLGATRDYENRILNVELLEEDEKKNRHFRDSFINELETVDEIPE
                     FTKISNIFRKENLLQEEVCENKLEEVIDDITRNSLRFPVSRSAKLQALTRGESGVVSG
                     FAYAIVRGFGDEHPYISELRTGNVFIKVKHPIYEDEEIIIGDMLVTECEIVSKASQNK
                     TENSKTNSEDKNKMSLGYGLCMGNNENKAISMAILDKSLEPNSEKLAQDEEFVLLHID
                     GIDSLGFISHFKLPHYVDFKADVERIK"
     gene            15832..16701
                     /locus_tag="PC545_002183"
     CDS             15832..16701
                     /locus_tag="PC545_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDF2456950.1"
                     /translation="MIYNYGFLDENTKKEIRRKILKAISIPGYQVPFGSRELPIAKGW
                     GTGGLQLTLSLIGKNDVVKVIDQGSDDSTNACNIRSFIASVSNVETTKDTLEATLIQT
                     RHRIPEEKLKSNQILIFQVPIPETLRIVEPSEVETRRMHSEEDYSRMWVYLYEDIVRF
                     NDISIAVEYPCKVNDRYLMNPSPIPRFDIKKLNMSDNLFLFGAGREKRIYAIPPYTKV
                     EPLEFEDYKFEEEKFEGKYCSLCNSTNTFLDEVYDSDTNEKYYSCSDTSYCEKVRLKN
                     NSIDVTIGGTWNE"
     gene            16694..17560
                     /locus_tag="PC545_002184"
     CDS             16694..17560
                     /locus_tag="PC545_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2456951.1"
                     /translation="MSKINDSKRLENIDKQVLIGRNINLIYGDGCEKCFESTGYESNN
                     ICKYCGSIVACNNVNLELYEGEVLGIVGESGSGKSTLLKILFFQEHANSGEVYISHYN
                     KDTNILELSDQKKRYIKNHFMGIVYQNPHLGLNLDFSSGGNIAEKLLMANLYNVEKIR
                     DRGKELLIKTNIPVERIDHKPKYFSGGMQQRVQIAKALSNNPPILLLDEVTTGLDVSV
                     QAEVLDLIREIQRELKISMIVVSHDFDVIKMLADRTIVMKNGSVIESGLTDQIMEDPQ
                     HPYTQQLINSLL"
     gene            17601..18314
                     /locus_tag="PC545_002185"
     CDS             17601..18314
                     /locus_tag="PC545_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2456952.1"
                     /translation="MKETILEVNNFSKDFELHALNKTITACSNISFEVSKGEFLGIIG
                     KSGAGKSTILKSIYKTYIPTTGEIIFNSEIYGNVDLAKIGDREMINLRKKEIGYVSQF
                     LKTLPRITAIELVVHSLIESGFEKDCSYDMAKDILNQFEIKENLWDAYPNNFSGGEKL
                     RLNLAQAMVKKPRLLLLDEPTASLDNQSKIYVKEKMLELKSQGTTMIGIFHDIEFMET
                     VIDKTFTMTKGTISESGVA"
     gene            18315..19484
                     /locus_tag="PC545_002186"
     CDS             18315..19484
                     /locus_tag="PC545_002186"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDF2456953.1"
                     /translation="MIKLIHNANIVLEDMILENGHLIIEGDTIKKVSNDKIDYAFDFN
                     EIDEIIDAKNLYVIPGIIDIHSDAIEKEIEPRPSTLLPFNMAFYELDKKLPANGITTV
                     YHSISLGDGVGVRSIDNSLKMIKNIDSYKNIDSKSINHKVHLRYEVLYYEGLEKVLEL
                     LDENKIDYLSIMDHSPGQGQYTNPTFYKEYATKVWGVTENYVDTWLDDLVNLHDNLDW
                     NKIANIIGIAKTKNINVASHDDDTLEKMDFIKNIGINVSEFPITLEVAKHSKKLGVLT
                     CLGAPNIVRGKSHNNNLKAMDAILEDCCDIVCSDYLPSAMIKSMCIVAERINNLNKAV
                     SLFTSNPAKAVGIYDERGSIKENKKADLVLVDIDSEYPKVVNTIVNGKTVYKREV"
     gene            19488..20324
                     /locus_tag="PC545_002187"
     CDS             19488..20324
                     /locus_tag="PC545_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDF2456954.1"
                     /translation="MLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEALVLGEHYGITIIPGIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFH
                     RGNGKVYVSLKYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            20325..21320
                     /locus_tag="PC545_002188"
     CDS             20325..21320
                     /locus_tag="PC545_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2456955.1"
                     /translation="MSFLQVKNVGKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDKDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKEVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDDGINKIAIRPEDIKVESNRDSKDKDIKVGEILDIE
                     FRGFNYRITVEYRSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            21338..23026
                     /locus_tag="PC545_002189"
     CDS             21338..23026
                     /locus_tag="PC545_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HDF2456956.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLVSLLKNAFM
                     DTSGNFIGISNFSKYVENPSLIASFKNSIFVSTVSSLISLCLAFIYSYAITRTNIKFK
                     DFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKFRDRVFLIVCSFITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAIFGTIFVFFSAYMIEKVDKFPKLRQTAYLLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNVVHFYSVCFITSNSSLKMLDK
                     EYELVAKSINIPFYKVFFNITVPMSITSILEIVVYYFVNSMVTVSALIFLYTPQTQTA
                     SISILKLDEIGYIGTSAAMAVLVLLTNIIVRLLYEFVTKKLKNNTQKWQQKDVA"
     gene            23038..24105
                     /locus_tag="PC545_002190"
     CDS             23038..24105
                     /locus_tag="PC545_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2456957.1"
                     /translation="MKFKKLVSLGVLSTLLLSVITGCSTDSSGSDSKSDSKTKGDLTV
                     YTAIEEDSIEPYLATFKEKYPDIKLNIVRASTGDITARLLAEKDNPQADVVWGVAATS
                     LLVADDQGMLEPYAPKGSEEIESAFKDDKEVPSWVGIDAWMTGITVNTKELSDKNIPV
                     PSSYEDLIKPEYKGLISMPNPSSSGTGYLTVSALIQIMGEEKAWQYMDKLHENIGVYT
                     QSGSAPATSAASGEYPIGISFGYRGIKLKEEGYPVEVVFPKEGSGWDIEANALVKKDN
                     IKEASKVFLDWAISKDVMNEYSKNYAVTTISTGNPIPEGFPKKPLEQMIDNDLKSAAK
                     NREDILNKWISKYDGKTEKES"
BASE COUNT         9709 a         2711 c         4339 g         7558 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tcttgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggtaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatgg taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatgtttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat gtaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaaagg gacaggttta
      541 taaaaagtta ggatataaag atatactaaa aaatttgtat aaaatggata caactgagat
      601 attaggaaca gataatttac actttccgga aggaattata aaaaattcac aaaaaaaagc
      661 atcaagagta tttaaaagtg attatacttg ctatttagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgtagtcc taaaagtaaa ataataacaa atagagactg
      781 tcatcagtct gttataaatg gatgtatttt aggagatata gatatagagt atgtgccttg
      841 tgagataagt gaagagacaa atatcttaaa agggatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcagt actgcttact tatcctacat attatggaat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaagatgg tagtgatagt
     1021 agatgaagca catggtgcgc atttagggct tagtgagagg cttcctaaaa cagctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccagcat ttacacaatc
     1141 gtcaatgata catatacagg gagaccgagt tgatatagat aaaatattga acatgttaag
     1201 aattacagaa tcttcaagcc cttcatattt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aagaattaat ggaagagctt ttaaataata ttataacttt
     1321 caaaaataaa gttgattcaa atattaatat atacgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaacatgg gtttaacagg ttatgaacta gaaaatattt tgagaaataa
     1441 atataacata caagtggagt tatctaatta ttatggagtt cttttaatat gtacaatagg
     1501 aaatactgct gaggatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 ctttaaattt gaaaagttag aaaataaaaa ataccctgtt gttattccaa agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagtatagg
     1681 taaagtttgt ggtgaatgta tagttccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta tctaatgttt tgtcattcta aaggcatgat
     1801 tgtgagtgga ttgaaagatg tagatttaaa ttttatacaa gtaatagatt tagaagacat
     1861 ttaaaaatta tgatataaaa gtagatagaa gtagatagtt taaaattgtg aagagaaata
     1921 aatagactgc ttatatagag atatctaata aaagtactaa aaaagtaaaa agtgtttgta
     1981 tggaggatat gaaaatgaaa ggtaagttaa taattataga gagtggttca gatgcaagtg
     2041 gaaaggctac tcagacagaa atattatatg aaagattaaa aaaagaaggc tatatgatta
     2101 aaaaggtaga atatccaaat tatgaatctc catcatctgc cttggtgaaa atgtacctaa
     2161 aaggagactt tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta
     2221 cagctgatag gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa
     2281 taattatcgc agatagatat acaacctcta atatggtaca tcaggcctct aaaatggata
     2341 tttgtgatag agaagagtat ttaaattggt tagctgatta tgaatttaat ttatttaaga
     2401 taccacaacc tgattgtgtt atatttttag atgtgcctat tgaatttagt aaaaagctta
     2461 tggagaatag aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca
     2521 tagagtattt agagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga
     2581 aaaaaataaa ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa
     2641 tttataaaat agttcttgat tcaataaaac ctatggagag ataaatatgt attttgaaaa
     2701 tattataggg caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact
     2761 taataatgcg tatatgtttg aaggaatgga tggaatagga aaaaagaaat ttgcagatga
     2821 attatcaaaa ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat
     2881 aaaaccagat ggaaatagta taaaaatagc tcaaatcaga aatttacaat cagatatagt
     2941 aataagacct cataaagact ataaaatata tataatcaat aatgctgaaa aaatgactgt
     3001 ggaagctcaa aatgcacttt taaagacgct agaggaacct ccaaactatg ctataataat
     3061 cttagttaca aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat
     3121 aaagttctca cctataccaa ttgaagactt aaaacgttat ttgataaata caggtataga
     3181 agaagaaaga gctcaattat tggctatttt ttcgagagga agtattgaga atgctctaaa
     3241 tctatctcag tcttctgagt tttcaatgat gagagaagat atacaacagt atattcagat
     3301 aatgttagat aaaaacatag tggagatttt aaatatacca aataatatgg aaaaatatag
     3361 aggtaagatt atagctttat tagatatgat gataaactat tttcgagata tcatcctatt
     3421 aaaagaaaat gtaaacaaga acatgttaat aaatgtagat aagctagttt tcattcaaaa
     3481 tatgaatgga aaaattagtt attctcaatt gtccaagatt attgatataa tagaagatgc
     3541 aaagagtaaa ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa
     3601 tatatacgag gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa
     3661 atatattatt ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa
     3721 actgcaagag gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc
     3781 gaattagttt ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta
     3841 tatctagaaa ataaggaaaa ggcaaaagaa acttttgagt tgtgccaaca aaaaataaaa
     3901 gaacatgagt taacaatgtt tttaatagat tgtgagtata catttgatag gaataagttg
     3961 attttttact ttacagcaga aggaagaata gattttagag aattggtaaa agacttagct
     4021 gctatattta aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct
     4081 ataggaggtc tagggccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt
     4141 caaccagttt caataaaaat ggcaaaagac caaagtctat ctctaaatcc aacaaaaata
     4201 tctggaatat gtgggagatt attctgttgc ttaaaatatg agcatgatgt atatagtgaa
     4261 gcaatagaaa aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg aaaaggaaaa
     4321 gttgtagaag tgaatccttt attagaacaa attaaagtgg aatttcaaga caagacagta
     4381 aagatataca caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt
     4441 ggtggtaaat gtaaggataa tgatggatta gatgaagcca cactaagaga gctaaagaaa
     4501 ttagaagatt aaatttaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa
     4561 agttattaat atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg
     4621 atttacaatt aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag
     4681 atgcagtttt attagccaat tttactaaaa taaaaaaaga tgccaaagta gtagatttgg
     4741 gtactggaac tggcattata cctattttga tagctggaaa aagtgaagct aaaaaaatta
     4801 ttggcgtgga aatacaagaa gatgtatatg aaatggcaac tcgttctata aaattaaatg
     4861 gattagaaga aagagttgag attataaatg aagatataaa atacatagac aaagtcttag
     4921 acataaatgg ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa
     4981 aaaatccaaa tgataaaaaa gcaatatcaa gacatgaagt aaggtgcaat ttggaagatg
     5041 taattagagc tgcatccaga ttggttatgc ctagaggaaa attcttcatg atacatagac
     5101 caacaagatt ggttgatata ataactttag ggagaaaata taaattagaa ccaaaggtta
     5161 ttcaatttgt gcatccaaga cctcaaaaag caccgaactt agttttggtt caatttgtaa
     5221 aagatggaag gccagagctt aaaatattgg acccattgta tgtttatgga gaagatggga
     5281 attatacaaa agaattaaaa gcaatatata ataataaaga tataggagag ttgtaaatga
     5341 gcgggaaatt gtacatatgt ccaacaccaa taggaaacct agaagatata acatatagaa
     5401 ctttaagaat attaaaagaa gtagacttga ttgcagcaga agatacaaga catagtataa
     5461 aacttttaaa tcattttgaa atctcaaaac cactaactag ttatcatgaa cataataagg
     5521 attcaaaggg agattatttg ataaataaac taattgatgg tgaaaatata gctctgataa
     5581 gtgatgcagg tatgcctggt atatctgacc caggagaaga gataataaaa caagctatac
     5641 aaaataacat agagatagaa gtattgccag gtgcaacagc ctttgtaacg gctttagttg
     5701 gctctggtat ggacacacat agatttattt ttgagggctt tttagataga gataaaaaag
     5761 ttaggaaagc acaattggaa gaattaaagg aagaaagtag aactataatt ttctatgaat
     5821 caccacatag attgaaggat acactaaaag atatgcttaa aattcttggt aataggaata
     5881 tttctataaa tagagaactt acaaaaaaat atcaagaaat aataagagaa aatatagaaa
     5941 ctaccataaa aatatttgag gaaaaagaag taagaggaga gtttgtatta atagtagagg
     6001 gttttcatgg tgagaagtca aagaaagaaa attatgatgg gcttacagat agggaatatg
     6061 ttttaaaact aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag
     6121 atagaaagtt aaagaaagat gtggtttata aacaagtttt agatttataa aacctgaatc
     6181 tgaggtgatt ttatggaaat agggaaaatt ataatagaga gtttaaagag ggaagataca
     6241 cataaaatat ttatagaatt agataataag catgaattag aaaaaataat tcctaaaata
     6301 agctgtatga agtctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacattct
     6361 ataaatgcat taaaagaatt agaaatagta ttaaatgata aggatttttt tccaatacat
     6421 ctaagaaaac atgtgtcaaa ctatttaaaa acatatattg aagaaggaat aaataagtta
     6481 catgtattaa agctgggaac atttttacat gatataggta agcctgacag tatgacctta
     6541 gatgaaaccg gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac
     6601 aatatgggaa taaagttaga gttatcaaca gagacatata gacttataag taaatatgta
     6661 agatatcata tgatactttt gtctctatat aagaaaaatg atttgagtag gaaagaatta
     6721 attaatgtat ttaacttagt agatgaggac actataggtg taataatgct tgggtatgca
     6781 gatatagtgt ctacagtaaa attattgggc aagagtggag aagtcagtgt gttaaagaca
     6841 tatatggaat atattttaac aaattacttg tataagagta attatacaca tgtataaatt
     6901 tagttaatat aattgagttt tagatgatta attgaagttt gaaatatacg gatttcaaaa
     6961 acataaaaga agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa
     7021 ttgaaatttt attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt
     7081 tatattacaa tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag
     7141 aaccaatttt ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta
     7201 cttttaaaag taagcaatca tcacctgtta tatggtgaca ttctacaatc ctagggtcct
     7261 ttgcagctga ctcaataaat tctgtatatc cattgcttgg aagagaaata tgaataaatg
     7321 cctttataac tctgcctaat gaatctgggt tgacaatagc tttatatcct tctataacac
     7381 cagactcttc taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt
     7441 ttcctaagtc cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat
     7501 ctgtaacatc cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt
     7561 ttttagaatc tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat
     7621 aaatgtgtgg ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta
     7681 catgattttt tatttcaaga acagcttttt tattatattt gcatctgcct tttatataca
     7741 atggtttaag aagctcttta actttgtaat ttgtaaatac cttcgttttc catgtataag
     7801 taggatggaa gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca
     7861 aatctgcctc tgccttattg tcttcgttat atattcttat aactttcttg taaccaggat
     7921 tatttatttt ttctggattc tcagaaatct ttatcttagg ttcaagaact cctttttcac
     7981 aagatgcagc taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct
     8041 ttgtacctac tccccatgaa tttatagtag ctccttctgc tttaagtgat gttatggtat
     8101 attcgtctag gtcattagat gcagttatgc tgatatctga aaagccagca ttatctaatt
     8161 gttttttagc ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag
     8221 gtttgtatcc tttttcagaa atatttttga atacttttat tgcattagga actccactgt
     8281 ttaatacatc ataagtatct acaagtagta ggcatttatc tggataaaca tcagcatatg
     8341 cttggaatgc ttcaagttca ttatcaaatt tttgtaccca actgtgagct tgtgtaccaa
     8401 ttataggtat atcaaacatt ttccctgcta gtacgtttgc agttccagca caaccaccaa
     8461 ctacggcagc cctagcccca tatatccctg catctggacc ttgtgcacga cgtaatccaa
     8521 attcaaatac agggtctcct tgagctgcaa agcaaactct agatgcttta gtagcaataa
     8581 gagactgaaa attaactatt gttaacaatg ctgtttcaat aagttgagct tgatataaag
     8641 gtgccttaac agtaattaat ggctcattag ggaacatgat agttccttcc tcaacagcat
     8701 atatatctcc tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat
     8761 ttaagttttt taagtactct aaatcttcat ctgaaaaatg aagattatct atgtattcaa
     8821 caacctgatc aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa
     8881 acatatcaaa tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag
     8941 taagctgata taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt
     9001 attaaatatt aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac
     9061 tttcaagtat aaagtattac ctaactatca taaaattatt taaagaggag gatgattata
     9121 ataggaaaaa tatggtttta tatggtatcg ataggaataa taggaagctt gatttctaaa
     9181 aatatgggtg aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca
     9241 ataagtctag ctggtatcat ggcactctgg atggggataa tgaatatcgc aaaagaatca
     9301 ggtttaattg aaaagatagg tcaaaaacta aatcctataa tgagaagatt atttccttct
     9361 attccaaaag gtcataaagc aatgtcttat atagttatga atattgcttt aaatatgttg
     9421 ggtgctggaa atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg
     9481 aataacaaga aagatacagc cactaatgat atgattatgt ttatggtaat caatatatca
     9541 tctatacaaa taattccatt tactataatc aaactaagga tggatatggg ggctcaaaat
     9601 ccaagtgaaa taatttttac gactttattt gcaaccataa tatctacagc agtagcaata
     9661 ataacatgta aattttttca aagaagatat agataaaggg gatgtagata catggagata
     9721 ttttcaaact tacttatacc aataataatt ctgtacatag tagtttatgg taaatataaa
     9781 aaaatagatg tgtatgatag ttttgtaaaa ggagcaattg atgggttaaa agcagcatgg
     9841 gatatcctac catatataat aggtatattt ttggcaatag gaatattcaa aactggtaaa
     9901 ggtcttgata tgttagaatg gctatttaca ccaatagcaa atatgatgag tataccaaaa
     9961 gaattaatag gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtac
    10021 agtgaacttg caaatagagt tggaatagga agtttagtag aaaaaatggg tgctacgatt
    10081 gtaggttctt ctgagactat tttttacaca atggcaatat actatggaag tctgaagata
    10141 aaaaatacta gacatacatt atcatgtgct atgattagcc atgttgcagg tgtcatagca
    10201 gctgttttta tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta
    10261 taatatagtg attatataga aacaaatagt aatacaaatt acaagctatg aaaagaagag
    10321 taaataaagg ttttttctat cagagagcta ggttaggtga aagctagtgt caaaagcttt
    10381 attgaagatg gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac
    10441 acgttatagt ctactaaggt atctataaaa tgtttctaca tttcatgtga aacataacta
    10501 ttatagatga attagggtgg taccgtgaat aatctcgccc ctatgttaac tcataggagg
    10561 cgatttttta ttatttaaat gaaagaatca tatgattacg aacgataaaa aattaggagg
    10621 taaataacat gagcaaaccg agtttttatg taacaacacc aatatactat ccaagtggag
    10681 gattacacat aggacatact tattcaacag tagctgcaga tacaatagct agattcaagc
    10741 gtttctgtgg atatgatgta aagttcttaa caggaacaga tgagcatggt gaaaaaattc
    10801 aaaagaaagc catagaacaa ggaatgtcag agatagagta tcttgatgga atgataaaag
    10861 atataaaggc cttatggaat actatggata tatcttatga tgattttata agaactacag
    10921 aaaaaagaca tacagacata atacaaaaga tatttactaa actatatgag caaggtgata
    10981 tatataaagg tgaatatgaa ggtagatact gtactccttg tgagagtttc tggacagaat
    11041 ctcaattact agaaggaaat aaatgccctg actgtggaag agaaacatat ttagtaaaag
    11101 aagaatctta tttctttaga ttatctaaat atgaagacag attaaaagag ttatttaaag
    11161 atgatagttt ctgttttcct gctgctagaa aaaatgaaat ggtggcaaac tttttagaca
    11221 aaggattaga agatttaagc gtaactagaa cgacttttga ctggggtata aaagttcctt
    11281 ttgatgaaaa acatgtaatt tatgtgtggg tagatgcttt atgtaactat ataacagcat
    11341 taggttatat gactgataat gatgaagaat ttaaaaaata ttggcctgcc aatgttcaaa
    11401 tagtaggtaa agaaatagtg agattccata caataatatg gccagcgctt ttaatggctt
    11461 taggtctaga agttccaaaa caagtatttg gacatggatg gatactattt gctgatgata
    11521 aaatgagtaa atcaaaaggt aatgtggttt atccagaacc tataatagaa agatatggaa
    11581 tagacacact taagtacttc ttattaagag agtttgcatt tgggcaagat ggaagttata
    11641 ctcatagaaa ttttgtaacg agaataaatt atgatttagc aaatgattta ggaaacttaa
    11701 taagtagaac tgtagctatg gttgaaaaat acaataatgg tataatacca acagcaaaag
    11761 tttcaactaa ttttgatgct gacttaaaag aacaagctgt ttcaactaga gaaaactttg
    11821 aagctgaaat ggataaaatg caattccatg aagcattaga gagtgtatgg aaattagtta
    11881 gaagaactaa caaatatata gatgaaacta tgccttgggc actagctaaa gatgaaacta
    11941 agaaagatga attagacaca gttttatata atttatgtga atctataaga ataatcgcaa
    12001 cactaataaa tcctataatg aatgagactg ctaataaaat atatgaacat ataggaataa
    12061 aaggtcaaga tgatataact acatgggaaa gtacaaaaac atttggtctt attggagaaa
    12121 atgtaaaagt atttaaaggt gaacctttat tcccaagatt agatgtagaa aaagagatag
    12181 aagaattaac taaaatgttc tctgggaaac ctccagtaga agaaaaacct ttagagcata
    12241 aagaagaaat tactattgat gatttagaca aaatagagct tagagtaggg aaaataataa
    12301 gctgtgaaaa acatccaaaa gcgaataaat tattagtatc acaagttaag attggaccag
    12361 aaacaagaca aatagtatca ggaatagctg aatactataa gccagaggat ttagtaggaa
    12421 aagaggttac agtggtatgt aacttaaaac cagttaaatt aagaggtgta gaatctcaag
    12481 gtatgatatt agcagcaggt gatgatggag acccatatgt tttaccattt acacaagggg
    12541 ctaaagatgg atgtgaggtt cgttagtagg taattaaata gtaaggagat taaagatgtt
    12601 atttgactca catgcacatt taaatgatga aagttttgat gaagatagag atgaacttat
    12661 tggttcatta aaagataaag gtgtagattt ggttgtaaat ccaggagcag atatagagac
    12721 gtcgataaca gcaattgaac ttgcgaaaaa atacgacttt atatattcag cagttggggt
    12781 acatccacat gatgtatcaa aactagatga tacagcaata gaaactctaa gaaaacttgc
    12841 aactgagaat gaaaaagttg tagctattgg agagattgga ttagattatt attatgatta
    12901 ttctccaaga gaagaacaaa aagagtggtt taagaagcaa attgaattag ctaatgagct
    12961 aaaacttcca ataataatac atgatagaga tgcacatgga gatacttttg agataataaa
    13021 gaatactaaa aatcctgaaa taggatgtgt acttcattgt tatagtggga atgtagagtt
    13081 agctagagaa tatgttaaga tgggttgtta tatatcaatt ccaggtacgg ttacttttaa
    13141 gaataataaa aaaactagag aagtagtaag agaaatacct ctagaaagat tattcataga
    13201 gactgattca ccatatatgt caccagagcc acatagagga aagagaaaca atccttctca
    13261 agtatctttt gttgctgata agatagccca ggaaaaaggg atatcttatg aagaagtatg
    13321 tagagttaca aaagaaaatg caaagaaatt ttttaatata aaatagacaa attaaatctt
    13381 ataatgatat atataataag tcattataag tataggatga gagtgcctca aaatgagttt
    13441 tttagtttat gaggcactct ttttgtatga aatcaaatat attttcatca tttcaacaat
    13501 gaaaaagaag tttatcatat tttgagatag tttgttttta tttatttaat tcatatttta
    13561 aaatatattt tcttaacatt gttaaaaaag aatttacaag atattcatta gaattaactc
    13621 aactctaata ttcacttaac ttaagttgtt tattatatta ttaaagagaa gtaaacgaaa
    13681 agtagattaa ggaggttgaa ttttgtatac agttgatagt actaagatat tgataaatat
    13741 gaacttagag aaactagaaa agatatactc aatgataaaa gatacatcaa gtatttctat
    13801 aattaaagaa cctaacttgg ctacagtaat ggtaagagct aatgaaagtg taaaaaacac
    13861 aacatttaat ttaggagaga tattagttac tgaatgtagt gtaaaggtag atgaaagctt
    13921 gggttacgga atagtttcag aaaacaataa taagaaagcc atttatttag cagttataga
    13981 tgcagtttta catagcaata acagtaaatt tgatgagtta aagaattaca taaataaaag
    14041 tgtatatgaa gagaacttaa ggtatgaaca agagattatt gatgaattta gcttgattaa
    14101 taaaacgaaa gttcaattca atgctatgga ttaaatagga ggagaatcat ggtaaattat
    14161 acacaagaaa tatatagaaa gatgctagat agtgcttcat ttcctgggaa attaaataat
    14221 atagataata taaatataga aaataataca aaactattga atggagcaat tggtatagca
    14281 atcactttat tagaccaaga agttaccttc tatatagaaa attatactca aaaagatata
    14341 aaaaatataa gagcacttac aatgtcaaat caaatagatt atagaaaatc agattatata
    14401 tttttagata ttaattcaga agtggatata ttacagatta aaataggttc ttttgaatat
    14461 cctgatgaaa gtgcaactat agtacatcaa gtaaatgatt tatcaacgta ctctcaaact
    14521 aagtatataa agcttgattt gagtggtcct ggtataaaat ctaaaaatag tatatatata
    14581 aatggaatta gagaagaatt tatagaaaga ttatctagca taaataaaga ttatccaata
    14641 ggaatagatt tgattttagt agataaaaaa ggagaaattg catttatacc aaggtcatca
    14701 aaattaagtt gggaggtatt atagaaatgg cttatgtagc agttaaaggt ggagaagaag
    14761 ccatattaca ggctaaaaat atacttgagt tttatagggt aaaaggagat agcttgccta
    14821 tagatgtaaa gcagattgag gaacaaatgt cttatgctgt agacaaagta atgtctgaag
    14881 gaagtttata caacaaggaa ctatcagcat tggctataaa acaagctcaa ggtgatatat
    14941 tagaagcctc tttctatgta agagctttaa ggtcaacaat gcctagaatt acatattcag
    15001 aacccataaa tacagaagaa atgagaataa aaagaagaat atcagctact tttaaagata
    15061 taccaggagg tcagtatctt ggggcaacta gagactatga aaatagaata ttgaatgtag
    15121 aattattaga agaagatgaa aaaaagaata gacattttag agatagtttt ataaatgaat
    15181 tagaaacagt agatgaaatc cctgaattta caaaaatatc taatatattt agaaaagaaa
    15241 atttattaca agaagaagtt tgtgaaaaca aattggaaga agttattgat gatattacaa
    15301 gaaatagttt aagatttcca gtttctaggt cagcaaagtt acaggcatta actagaggtg
    15361 aaagtggagt agtgtcaggt tttgcttatg ctattgtaag aggttttggt gatgaacatc
    15421 catatataag tgaacttaga actggtaatg tatttataaa agtaaaacat cccatctatg
    15481 aagatgaaga aataataata ggggatatgc tggttactga atgtgaaata gtttcaaaag
    15541 catcacaaaa taagactgaa aattctaaaa ctaattcgga agataaaaat aagatgtctt
    15601 taggatatgg tctttgtatg gggaataatg agaataaggc tatatctatg gccatactag
    15661 acaaatcttt ggagccaaat agtgaaaaac tagcacaaga tgaagaattt gtattgcttc
    15721 atatagatgg aatagattca ttaggattta tatctcactt taagcttcct cactatgtag
    15781 attttaaagc ggatgtagaa agaataaaat agtttgagga ggtaagttta ggtgatatat
    15841 aactatggtt ttttagacga aaacacaaaa aaagaaataa gaagaaagat attaaaagca
    15901 atatcaatac ctggttatca agttcccttt ggttcaagag agttgcctat agctaaggga
    15961 tggggtactg gaggtttaca attaacacta tcattaattg gtaaaaatga tgtagtaaaa
    16021 gtaattgacc aaggaagtga tgattctaca aatgcttgta atataaggag ctttattgca
    16081 agtgtaagta atgtagaaac cacaaaagat acattagaag ctacattgat tcaaacgaga
    16141 catagaatac cagaagaaaa acttaaatct aatcaaatac taatatttca agtaccaata
    16201 ccagaaaccc ttagaatagt agaacctagt gaagtagaaa caagaagaat gcattcagaa
    16261 gaagattata gtaggatgtg ggtatatctt tatgaagata tagttagatt taatgatata
    16321 tctatagcag ttgaatatcc atgtaaagta aatgatagat atttaatgaa tccatcacct
    16381 atacctagat ttgatataaa aaaattaaat atgtcagaca atttattctt gtttggtgca
    16441 ggtagagaaa aaagaattta tgctattcct ccatatacaa aggtagaacc tttagagttt
    16501 gaggattata agtttgaaga ggaaaagttt gaaggaaaat actgtagttt atgtaacagt
    16561 acaaatacat ttttagatga agtatatgac agtgacacta atgaaaagta ctatagttgc
    16621 tcagatacaa gttattgtga aaaagttaga ttaaaaaata acagtataga tgtgacaata
    16681 ggaggtactt ggaatgagta aaataaatga ctctaaaaga ttagaaaata tagacaagca
    16741 agtattaata ggaagaaata tcaatcttat ttatggagat gggtgtgaaa agtgttttga
    16801 aagcacagga tatgaaagca ataatatatg taagtattgt ggttctatag ttgcatgtaa
    16861 taatgtaaat ttagaacttt atgaaggaga agtccttgga attgtaggtg aatctggttc
    16921 aggaaaaagc actttattaa aaatactgtt tttccaagaa catgcaaata gtggagaagt
    16981 atatatatcc cattacaata aagacacaaa tatactagag ctttcagacc aaaaaaagag
    17041 atacataaag aatcatttta tgggtattgt gtatcaaaat ccccaccttg gtttaaattt
    17101 agactttagt agtggtggta atatagcaga aaaattattg atggcaaatt tatataatgt
    17161 tgaaaaaata agagatagag gcaaagaact tttaataaaa actaatatac ctgtagaaag
    17221 aatagaccat aaaccaaaat actttagtgg gggaatgcaa caacgagttc aaatagcaaa
    17281 agctctttca aataaccctc caatattgct attagatgag gttacaacag gtttagatgt
    17341 ttctgtgcaa gcggaagtat tggatttaat aagagaaatc caaagagaac ttaagatttc
    17401 tatgatagtt gtatcccatg actttgatgt tataaagatg cttgctgata ggacaattgt
    17461 aatgaaaaat ggaagtgtaa ttgagtctgg tcttacagac caaataatgg aggacccaca
    17521 acacccatat actcagcaac ttataaattc attgctataa atatcgtttg taaataagca
    17581 aaatgggggt ttttaataaa atgaaagaaa ctatacttga agtaaataat tttagtaaag
    17641 actttgagtt acatgcattg aacaagacta taacggcatg tagtaatata agttttgaag
    17701 tttcaaaagg tgagttttta ggaataatag gtaaatctgg agcaggtaag tctaccatat
    17761 taaaaagtat ctataaaaca tatataccaa caactggaga aataattttt aattcagaaa
    17821 tatatggtaa tgtagacctg gctaaaattg gagaccgaga aatgataaat cttagaaaga
    17881 aagaaatagg atatgtatca cagtttttaa agacacttcc acgaataact gcaattgaac
    17941 tggtagtaca ttctttaatt gagagtgggt ttgaaaaaga ttgttcgtat gatatggcaa
    18001 aagatatact taaccaattt gaaataaagg aaaatttatg ggatgcgtat ccaaataatt
    18061 tttctggtgg agaaaaatta agattaaatt tagctcaagc tatggttaaa aagcctagac
    18121 ttttgttact tgatgaacca actgcatcac ttgataatca atctaaaata tatgtaaaag
    18181 aaaaaatgtt ggagcttaaa agtcaaggaa ctacaatgat aggaattttt catgatatag
    18241 agtttatgga aactgttata gataagacct ttactatgac aaaagggact ataagtgaaa
    18301 gtggggtagc ctagatgata aaactaattc ataatgcaaa tattgtatta gaagatatga
    18361 ttttagaaaa tggtcattta atcatagagg gagatacaat aaaaaaagta tcaaatgata
    18421 agattgatta cgcatttgac tttaatgaga tagatgaaat aatagatgca aaaaacttat
    18481 atgtaatacc tggtattata gatattcaca gtgatgccat tgaaaaggaa atagagccga
    18541 gaccaagtac attattacca tttaatatgg cattttatga actagataaa aaactacctg
    18601 caaatggaat aactacagtt tatcattcta tatctttagg ggatggagta ggagttagaa
    18661 gtattgataa ctctttaaag atgattaaaa atattgattc atataaaaat atagatagta
    18721 agagcataaa tcataaagtt catttaagat atgaagtgtt gtattatgaa ggtttagaaa
    18781 aggttttaga actattagat gaaaataaaa tagactactt atcaataatg gaccattctc
    18841 caggacaagg tcagtataca aatccaacat tttataaaga gtatgctact aaagtgtggg
    18901 gagttacaga aaattatgta gatacatggt tagatgattt agtaaatctg catgataatt
    18961 tagattggaa taaaatagct aatattatag gtatagccaa aactaaaaat ataaatgttg
    19021 catcacatga tgatgataca ttagaaaaaa tggatttcat aaaaaatata ggcataaatg
    19081 tatctgaatt tcctataact cttgaagttg caaaacattc aaagaaattg ggtgttctaa
    19141 cttgtttagg tgcaccgaat attgtaagag gtaaatctca taataacaat ttaaaagcaa
    19201 tggatgctat attggaagat tgttgtgaca ttgtgtgttc ggattatctt ccatcagcta
    19261 tgataaagtc tatgtgtata gttgcagaaa gaataaataa tttaaataaa gcagtcagtt
    19321 tatttacctc aaatcctgct aaagcagttg gtatatatga tgaaagaggt agtataaaag
    19381 aaaataaaaa agcagattta gttttagtag atattgatag tgaatatcca aaggttgtaa
    19441 atactattgt aaatggaaaa acagtttata aaagggaggt ataaagattg ctaacagagt
    19501 tacattgtca tacaagtata tcagataata attttactac tgaagaaata ataaaaaaag
    19561 cagcagaaaa gaatataaat atcttagcaa taacaaatca tgacacatta tctggattgg
    19621 atgaagctct tgtgcttgga gagcattatg gaataacaat aataccaggt atagaaattt
    19681 cagcctatga ttataaaaat agaaaaagag ttcatattct tggatataac atagatttaa
    19741 acagtcaaga aataaaaaat ttgtgcaatc caatggtatg tgacagacat aaagcatctg
    19801 ttaagatggt aaataaaatt atagatttag gatacaaaat atctctggaa gatgtaaaga
    19861 aatattcatc aaagactgga atatttaaac aacatataat gaaagctctt atagataaag
    19921 gatatacaga taatatatat tcaaatctat ataaagaatt gtttcataga ggaaacggca
    19981 aggtatatgt atctttaaaa tatgtggatt acagagatgc aataaaagct ataaaaaatt
    20041 ctggtggaat ttgtgtgtta gctcacccgg gtcaaatgga taattttagc gctattgaag
    20101 agatggtaaa agtaggatta gatggaatag aagtatatca tccatctcac aacaaagaac
    20161 tagaaaaaga gtcgctttct tatgctaaga agtataatct agtggtcaca ggaggttcag
    20221 attaccacgg attttattct caacataatt atgaacttgg aagcaagagc ttaaataaag
    20281 aagatttatt taagtttagg ttagctttag gaggggtatt ataaatgtct tttttacaag
    20341 taaaaaatgt aggtaaaagt tatggacaag ttaaggtgtt aaaagacata tctattgata
    20401 ttgaaaaagg agagtttatt tgtttactag gtccaagtgg ttgtggaaag agtacactac
    20461 ttagaataat tgcagggctt gaggataaac atggtggaaa aataataata aatgataagg
    20521 atatgactaa ttcaccacct gaaagtagaa attttggtat agtttttcaa tcatatgcat
    20581 tatttccaaa tatgaatgtt tacaaaaaca tagcctttgg actagaaaat aaaaacataa
    20641 gtaaatctaa tattgataaa aaagttaaag aagttctaga agttgtagaa ttaagtggat
    20701 atgagaaaaa atatccatca caattatcag gtggacaaca acaaagggtt gcattagctc
    20761 gtgcgattgc tttagagcca gactttttac ttcttgatga acctttatct gcattagatg
    20821 caaaagtaag acttaaactt agggagcaga ttagaagttt acatagaaaa ctaggcatta
    20881 caactataat ggtaacgcat gaccaagaag aagctctttg tttagctgat aagatggttg
    20941 ttatgaatag aggagaaata atacaagttg gaacgccaaa ggaggtatat aaaaatccag
    21001 aaacaccttt tgtagctgat tttataggaa caataaattt tatagatgat ggtattaata
    21061 agatagctat tagaccagaa gatataaaag tagagtctaa tagagattct aaagataagg
    21121 atataaaagt gggagaaatt ttagatatcg aatttagagg atttaactat agaattacag
    21181 ttgagtatcg ttctaagcaa atgaaattag atgtagtttc caaagttgca gaacaaatga
    21241 aactgtgcat aggttctaaa ataaacttta agatacctaa agaaggaatt gtacaatata
    21301 aatcagaggg atgtgcttaa tagctagggg tgaataaatg ataacatcaa aaaataaaag
    21361 aagaataagt gatagcacaa tacaaaaata tttcttgatt ttttcggtaa ttgtactaat
    21421 agtatttata ttatttccat tagtatcatt attaaaaaat gcatttatgg atacaagtgg
    21481 aaattttata ggaatttcaa actttagtaa atatgttgaa aatccaagtc ttatagcatc
    21541 atttaaaaat tcaatttttg tatctacagt ctcatcttta atatcactat gtttagcatt
    21601 tatatattca tacgcaataa caagaacaaa cataaagttt aaagattttt ttaaaactat
    21661 gggaatgttt ccactttttg caccaactat gttatatggt atttcactta tatacctttt
    21721 tggaaataag ggaatcttta caaccatggg tctggaagta ccactatatg gtccattagg
    21781 aataattatt tctcagattc tatttacatt tccacaagct tttttaatat tatctgtagc
    21841 actttcgatg gctgattata gactttatga agcagcagat agcttgggtg ctagtgaatt
    21901 taagaaattt tgcaaaataa caattcctgg tataaagtac tcattactta gttcattctt
    21961 tgtatccttt atacttgcat ttacagattt tggagcagct aaggtagtcg gaggaaacta
    22021 caatgtactg gctactgaca tatataaaca agtagttgga caatttaaca taccaatggg
    22081 agctactgtt tctatggtaa tgttaattcc tgtactaata gcatttacat tagataagat
    22141 ggcaagtaag aaacaaggta tgaccataac tgcaaagtct atgccatata agattaagga
    22201 aaataagttt agagatagag tatttttaat agtatgtagt tttataacat tgctaatttt
    22261 tattttaata ttcgtttcag ttataggttc agttataaag ttatggccat ataatttaag
    22321 ctttacaatg gaacactata catttaaagg tattttggga agtggaataa aaacatactt
    22381 aagcagtttg aaaatatcaa ctttaacagc aatatttgga actatatttg tattttttag
    22441 tgcttatatg atagaaaagg tggataagtt tcctaaatta agacaaacag cttacctttt
    22501 atcaatgata ccaatggcct tacctggctt agttttaggt atatcatata taactttttt
    22561 taactcagca agtaatccat taaatttttt atatggaagt acagcaatat tagttatagt
    22621 aaatgtagtt catttttatt cagtttgttt tataacctca aattcatctt taaaaatgtt
    22681 ggataaagag tatgaattag ttgcaaaatc cataaatatt ccattctata aagtattttt
    22741 taatataaca gttccaatgt ctataacatc aatacttgaa atagtagtat attattttgt
    22801 aaattcaatg gttactgttt cagcattaat atttttatat acacctcaaa ctcagactgc
    22861 atcaatatca atactaaagt tagatgagat aggatatata ggaacatcag ctgctatggc
    22921 agtattagtg cttttaacaa atataatagt aagacttttg tacgaatttg taacaaagaa
    22981 attaaaaaat aacacacaaa aatggcagca aaaagatgtt gcatagggga ggtaaaaatg
    23041 aaatttaaga agctagtttc tttaggagta ttatcaacac ttttattaag tgtaataaca
    23101 ggatgtagta ctgattcatc aggttcagat tcaaagtctg atagtaagac aaaaggggat
    23161 ttaacagttt acacagcaat tgaagaagat agtatagagc catatttagc tacatttaaa
    23221 gagaaatatc cagatattaa attaaatatt gttagggcat caacaggaga tataacagca
    23281 agattattag cagaaaagga taatcctcaa gcagatgttg tatggggagt tgcagccaca
    23341 agcttattgg ttgcagatga ccaggggatg ttagaaccat atgctccaaa aggttctgaa
    23401 gaaatagagt ctgcatttaa agatgataaa gaagttcctt cttgggttgg gatagatgct
    23461 tggatgacag gtataacagt aaacactaaa gaattaagtg ataagaacat cccagttcct
    23521 agttcttacg aagatttaat aaagccagaa tataaaggtc ttatatctat gcctaatcca
    23581 tcttcttctg gtacagggta cttaacagta tcagccctta tacaaattat gggtgaagaa
    23641 aaagcatggc agtatatgga taaattgcat gagaatattg gtgtatacac tcaatctggt
    23701 tcagctccag ctacatcagc agcttcagga gaatatccta ttggtatatc ttttggatat
    23761 agaggtataa agttaaaaga ggaaggctat ccagtagaag tagtatttcc aaaagaaggt
    23821 tctggatggg atatagaagc aaatgctctt gtgaaaaaag ataatataaa agaagcatca
    23881 aaagtatttt tagattgggc tataagtaaa gatgtaatga acgaatactc taagaactat
    23941 gcagttacta ctatttctac aggaaatcca ataccagaag gattccctaa aaagccatta
    24001 gagcaaatga tagataatga tttaaaatca gcagcaaaaa atagagaaga tatactaaat
    24061 aaatggatat caaaatatga tggtaagaca gaaaaagaga gttaagtaaa actatacaaa
    24121 tggagtaaaa agaaagaatt tttttaagta gattttatta taataatata agttgagaat
    24181 aatataggtc aagaaaagtg aatattctat aaaaatgtat agagtaagta taccattcag
    24241 tttgaatggt atatttttta tttttagaga ttaatttaga aatagatata ctacagttta
    24301 aaatagtata ttttaat
//
LOCUS       DAMJVI010000053        24284 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.055, whole genome shotgun sequence.
ACCESSION   DAMJVI010000053 DAMJVI010000000
VERSION     DAMJVI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.055"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            76..897
                     /locus_tag="PC545_002191"
     CDS             76..897
                     /locus_tag="PC545_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDF2456958.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKDLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGMPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            918..2114
                     /locus_tag="PC545_002192"
     CDS             918..2114
                     /locus_tag="PC545_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDF2456959.1"
                     /translation="MFIEFFYLLRSRGLNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDAVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWILKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFKRKYSKIVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            2281..5679
                     /locus_tag="PC545_002193"
     CDS             2281..5679
                     /locus_tag="PC545_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2456960.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNILLTYPEIEFIEKPFILQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSDGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELRMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLQNNITEEVEF
                     IVGEGERILNINIWPDFVDDFSVHLVNPSNNQTQAISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWKIVFEPIDIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKGARRLSNQSY
                     PNNSSGFGFLNLSDIDLYTLSNINQDLETEDIGYRSINKSFKDEENSYKFIDGYNMQI
                     HNDLENEIYISKNASRQSGILSGIDIVHTPEFEEELAGLGMSQRFFKISDSLGVLSIN
                     NTDYNSIQRVLQLPSIIRTVSTTKMTLLGEINRGTFGGVVATEEMGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHPDFIYPDGTSKIVYLWDQTKEGTPPDGFYIGTEYTREDIN
                     RAIAENDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGIAEDSELIIIKLGKIDDFYNS
                     AMLFAASQYAYKKAFELGRPLVINMSLGTSSLAGLTNRSNSEKAFFTRGLCITAGAGN
                     EGNTQTHTSGIIPYVGGSVEVELELNEDEEELSLELWLNRPDKADVIIVSPTGEESKS
                     VGISNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRELLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINGSLWQSSSRG
                     PTIEDRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            5689..7362
                     /locus_tag="PC545_002194"
     CDS             5689..7362
                     /locus_tag="PC545_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2456961.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPLDFDET
                     ILNNIIQVAWWEESEPMSSLIEITNNVNNGETITTAAETDYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILVGNGRINSQYRGITTESDLIVVKLKSYTDTYYAGRINYSV
                     SDFLAAITYVTNIARTENKPLIINLTIGVKSSAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYSPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWILRLTSELI
                     INGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVASGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQDNV
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            7635..7829
                     /locus_tag="PC545_002195"
     CDS             7635..7829
                     /locus_tag="PC545_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HDF2456962.1"
                     /translation="MESKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVEEAKDWVDNGSKL"
     misc_binding    7998..8231
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8308..9705
                     /gene="asnS"
                     /locus_tag="PC545_002196"
     CDS             8308..9705
                     /gene="asnS"
                     /locus_tag="PC545_002196"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDF2456963.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFIRITYTKAVELLIESGHEFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(9878..11053)
                     /locus_tag="PC545_002197"
     CDS             complement(9878..11053)
                     /locus_tag="PC545_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456964.1"
                     /translation="MNLEKIKKFFGFPLDKKHKDNVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFVTPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKFGESVIVFGGCFLF
                     LNLFLPGIQTIPGNEFSNIVNTFFITSLAIFVSTMLYRNRVFTYYDKLLISQQYEEIS
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCNEADAKLKSECLRKII
                     ESCNFERDDVPLKCVTVSIGVYTKKIGKTITLNEFISNCDKALYTAKEQGRNCVAFY"
     gene            11472..12836
                     /locus_tag="PC545_002198"
     CDS             11472..12836
                     /locus_tag="PC545_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2456965.1"
                     /translation="MGEKKINNMNYSHNNYSKKTGEPTRKIKNSHITLKSKEEKICSS
                     CNKENNTKDNYCKFCGNELYEIASLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTI
                     FILFIIALIFKAIITIQFNDISYLVNPAHIILALNLGQISVSMSTMMGSGFINANIGL
                     LILLIIPILVLLISNLIFMRKRCRDSKTVLANSLGVGIFYGLVLAILSIFTNVKTSSH
                     SMLEYGYALEYSYEFFSVLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIRTFIL
                     GYVLVLVILLVLTISDSSYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIIN
                     STVSMFTLSSSSLFGDTKLMFYAMGALSMLILLLNGYKLRFKYNTDSIRPIIVFSIYY
                     AFLMGILALFSTFILDSNINFFNTTNYGTTLIMQFKVLQAIVISFVYSFVISLIGYKL
                     NSAD"
     gene            12858..13349
                     /locus_tag="PC545_002199"
     CDS             12858..13349
                     /locus_tag="PC545_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2456966.1"
                     /translation="MEGNTNNKVDDKFSKVKKKFGSGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSINDNSFDKFCDELLELDKLIYEKNMEINEMEMAESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIICGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            13621..14292
                     /locus_tag="PC545_002200"
     CDS             13621..14292
                     /locus_tag="PC545_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDF2456967.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCVSTTLSGYTPYTKTLEG
                     PDFVLMKRLVKDLEIPVIAEGKVNTPQDLKKVFELGVHSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            14377..15243
                     /locus_tag="PC545_002201"
     CDS             14377..15243
                     /locus_tag="PC545_002201"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDF2456968.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            15276..16778
                     /locus_tag="PC545_002202"
     CDS             15276..16778
                     /locus_tag="PC545_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2456969.1"
                     /translation="MVGFTWIDFAILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIAVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YRMPEVTSWAYSLITITTSVVVGTIVSAIEAKVKAKVSLPKAETTVYYEK"
     gene            16908..17360
                     /locus_tag="PC545_002203"
     CDS             16908..17360
                     /locus_tag="PC545_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDF2456970.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEKGDFL
                     PLDGKNNGYMELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            17520..18323
                     /locus_tag="PC545_002204"
     CDS             17520..18323
                     /locus_tag="PC545_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456971.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESIIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NTGCTTLSKHYNIENKYVIWIQGESDAKYGSDVLYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACSECDILCVGSKIAMQFHDRGLTIDDVHYTQE
                     GLNMLGEDLCKNIYKYHITKEKSVLSDTIDLSEARKYICELEKISKRFV"
     gene            18381..19268
                     /locus_tag="PC545_002205"
     CDS             18381..19268
                     /locus_tag="PC545_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2456972.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGFIIDKSSLDTEAKEKGGEGIL
                     AKIKDIVKKYIEENEISGICISTAGMVDPVEGKILFALEELIPNYKDMQLKKEVEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNVNGSSFQELASTSSLIKKVAKIKNLNENDLNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            19287..20135
                     /locus_tag="PC545_002206"
     CDS             19287..20135
                     /locus_tag="PC545_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2456973.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            20198..20887
                     /locus_tag="PC545_002207"
     CDS             20198..20887
                     /locus_tag="PC545_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2456974.1"
                     /translation="MSKLKDYNIDKVIIFNNLSTKTKNQLKEKVKLKRLQKKEILFYE
                     KDSLDSVYILLEGKVSIFKISENGERKVIFILNSGEVINDITLDASKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQNDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            complement(21217..22407)
                     /locus_tag="PC545_002208"
     CDS             complement(21217..22407)
                     /locus_tag="PC545_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2456975.1"
                     /translation="MSNVKRKDSKNRNLRNGESQRKDGRYVYKYTDIYGKPQFIYAWK
                     LVPTDKTPAGKRDDISLREKEAQIKKDLDDGIDTAGGKMTVCQLYDKKNSQRKNIKRA
                     TEKGRQYLMNALKNDPLGMRAIDTVKQSDAKEWAIRMSERGYAYKTIDNYKRSLKASF
                     YMAIQDDCIRKNPFEFKLSDVLEDDTEPKVILTPEQEERLLAFMETDKIYSKYYDEVV
                     LLLETGLRISEFCGLTTHIDMQNRILNIDHQLLRDSEIGYYIDTPKTKNGKRKIPLTE
                     RAYQALQRILKNRVKAQPLVVGGYSNFLFLNREGLPKVAGNYVGMVRGLIKKYNKYHK
                     DKLPNITPHSFRHTYCTNMANKGMNPNTLQYLMGHANITMTLGYYAHGTFQSAKAELE
                     RLAC"
     gene            complement(22485..22688)
                     /locus_tag="PC545_002209"
     CDS             complement(22485..22688)
                     /locus_tag="PC545_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2456976.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENIDAGWVI
                     VNGNRIQIKRKQFEKIIDTLDEI"
     gene            complement(23070..23318)
                     /locus_tag="PC545_002210"
     CDS             complement(23070..23318)
                     /locus_tag="PC545_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2456977.1"
                     /translation="MKHTFKKPSYYLLSQAMDGDEKAIEKVLAFYDPYISKCCLRPLY
                     DEYGNVYIVVDMELKGLIREALIKMILGFDIALEIEEE"
     gene            complement(23322..23741)
                     /locus_tag="PC545_002211"
     CDS             complement(23322..23741)
                     /locus_tag="PC545_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005361151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2456978.1"
                     /translation="MKPSDFQKTVQCRFESCLKKVVRHVVKDYQQGLKRRKEKEISFC
                     ELPEIMVENLAVWDDYETDYTIFNVCGNDIRVYDDELAEALKQLSERNRETLLMYYFL
                     EMNNEEIAKKQNISRSGVFQNRHNSLALMRKLLKEKR"
BASE COUNT         9113 a         2913 c         4367 g         7891 t
ORIGIN      
        1 ttattatttt tatatcaaaa atagtaataa agtctatata catttagaaa atactgttta
       61 aaaggagtag gatttatgat aaaaaaagag atatctaact ttagaggaag tagtgattat
      121 gtagtatcac cagaattgat ggcatcagtt aatgttgcta ttgctcttga aaagccactt
      181 ttgattaagg gcgaacctgg aacaggtaaa actatgctag cacaagcaat ttcaaatgaa
      241 ttaaaaaaag atttagtaat ttggaatata aaatcaacta caaaagcaca agaagggctt
      301 tatgtatatg atactgttca aagactttat gatagccaat ttggaggaga aggtgtagat
      361 gatattagca agtacataaa atatggtaaa ctaggagagg cctttagctc aaatcaacaa
      421 gtaattcttt taatagatga aatagataag gcggatttag aatttccaaa tgacttgctt
      481 tgggaattgg ataaaatgga gttttacata aatgagacaa aagaaactgt aagagctaaa
      541 caaagaccaa tagttattat aacttcaaat gcggaaaaag aacttccaga tgctttttta
      601 agaagatgca tatttcatta tatagaattt cctgatagag acatgatgga agaaatagtt
      661 aaagtacatt ttgataaagt agaagaacac ctattggaac aagtaatgac tactttttat
      721 tggattagaa gtttaaagga catacaaaaa aagcctagta catcagagtt aatagactgg
      781 atacaagcat taactttgag tggaatgcct attgaaaaaa tagaaaaaga agttcctttt
      841 gcaggaatac tacttaagaa taatgaggat attgaatcta tgcaaagaca cttatagtta
      901 ataggagctg gataattatg tttattgaat ttttttattt attgaggtca agaggattaa
      961 atgtatcttt aaatgaatgg atgactttaa ttgaagcatt ggataaagga ctgtgctatt
     1021 caaacttttc aaatttttac tatctttgca ggatgatttt aataaaaagt gaaagtgatt
     1081 ttgataagtt tgatgcagtg tttttggagt attttaaagg tatagaacac caagaagaaa
     1141 ttccagaaga gattatgaat tggcttaaaa aaccagatat agatttagaa gagtttgaaa
     1201 gattagaaca aaatccaaat ataaatcttg ataaattaag agctaaattg gaggaacgaa
     1261 taaaagaaca agattctcct cataatggtg gtaattattg gataggcaca ggaggcactt
     1321 cagagttagg acattcagga aaagggcaaa ctggtatacg tataggtgga aattctactt
     1381 atggtagagc tgttcttgaa gttgcaggag aaagaaaata tagagatttt agagatgatg
     1441 aagttttaaa tatgcgtcaa tttcaagttg cactaaagag acttcgtcaa ttttctacta
     1501 gaatagatgc tccaaagaca gagcttgatt tggataagac tattgaagag acttgtaata
     1561 atgcaggata tttaaagtta ttttttgaga agccaagaaa aaatacagtg aagcttttat
     1621 tgcttatgga ttcaggtggt tcaatgagag ggtatagcac tttatgtaat acattatttc
     1681 agtctgtaag taaatcaaat cattttaaag atgtaaagat atattatttc cataactgta
     1741 tttatgatag attatttaca acacctgaat gttggttaag taaatctatt aatactgaat
     1801 ggatattaaa aaatatagat aaaaactata aagttataat tgttggagat gcttctatgt
     1861 cgccttctga gttgcttcat attggtggta actatcgtgg tccatataat tacacacctg
     1921 gtatagagtg gcttaaaagg tttaagagaa agtacagtaa aattgtatgg atgaatccag
     1981 aacttagaga tggatgggat tcaattaatt attggtatca aactcaaaga atgatacaaa
     2041 gtgaatttaa tatgtttcca ttgactgtaa aaggtcttga gaaagcattg aaaaatttaa
     2101 tggtttcaag ataaagtaaa tataaatgat ggtatgattt attcaaaatt gtaccatcat
     2161 tatttatttt tttaatatac ctattataaa ttgtataact gaaacttaaa aaattatata
     2221 gtctaataca ttttagactc acacaaaaca tatacattaa taataatttt agaagtcgag
     2281 gtgattataa taaattatga attaattgta aaatacaatg gagatatatt gaggttagaa
     2341 gaagaattag gtgtatcagt agagatactt aattcatcat atgcaataat aacatcaagt
     2401 aatgaagaag atgtaaatat acttttaact tatccagaaa ttgaatttat agaaaagcct
     2461 tttatattac agactcagga tgtgcaaagt ttttcaagta caggaataac aggatttaaa
     2521 aatagaactg gtttaactgg aaaaggaact ataattggta taattgattc gggaatagat
     2581 tatactctac ctgtgtttag agatagtgat ggaaggtcta agatattata ttattgggac
     2641 caatctattc aaggaaatcc accagaaggt tttagagaag gaacattata tactaatgaa
     2701 gatataaata atgcaataga tggaagtatg tatataccta tttcaacaac tagcttacat
     2761 gggacacatg ttgcaggtat ttgtgcaact attgcaagtg atgccagaat aatagtggtt
     2821 agagttggaa atatacaaac cgatattttt tcgagaagta cagagtttat gagggcaatt
     2881 aaatttattt tagatagggc attagaatta agaatgcctg ttacattaaa tataagttat
     2941 ggaagtaatg aaggttcaca tagaggaaca tctttatttg aacaatatat agatgatatg
     3001 tgtttatttt ggaaaaacaa tatagttgta gcagctggaa ataatgcaga taaaggaggt
     3061 cataaaagga ttaggcttca aaataatatt acagaagaag ttgagtttat agttggggaa
     3121 ggagagcgta tattaaacat aaatatatgg ccagattttg ttgatgattt tagtgtacat
     3181 ttggtaaacc catcaaataa tcaaacacaa gcaatctctc taacttcagg tgagataagg
     3241 aatactttgg gtgaaactag aataactggt tatttttacc ctatagcacc gtattctctc
     3301 acaagaagag ttactctaca attaagttca aatacacaga taactcctgg gttatggaaa
     3361 attgtatttg aaccaataga tatagtaact ggtaatgtaa atatatattt gccaacttct
     3421 gaaggtctaa atagaaatac tagatttctg attcctactc aagagcttac tgtaacagtt
     3481 cctggaacag caagtagagt tataacagta ggtagtttta attcaagaac agatatagta
     3541 tctatatttt ctggtgaagg agatacacaa ttaggtgttt ttaaacctga tttattagct
     3601 ccaggtgaag atatagtatc ttttttacca ggaggtacaa gtggagcatt aactggaact
     3661 agcatggcta cacctcatgt tacaggggtt tgttcattgt ttatggaatg gggaattgta
     3721 aatggaaatg atttattttt gtattcacag aaattaaggg cattacttct aaagggagca
     3781 aggagattaa gtaatcaatc ttatccaaat aattcatcag gatttggttt tttaaattta
     3841 tcagatatag atttgtacac tctatctaat ataaatcaag atttagaaac agaggatatt
     3901 ggatatagaa gtattaataa gtcatttaaa gatgaggaaa atagttataa gtttatagat
     3961 ggttataata tgcaaataca taatgactta gagaatgaaa tttatataag taaaaatgca
     4021 agtagacaat caggaatact atcaggaata gatatagtcc atactcctga atttgaagaa
     4081 gaactagctg ggttaggtat gagtcaaagg ttttttaaga taagtgattc attaggtgta
     4141 ctatctataa ataatacaga ctataatagc atacaacgag ttttgcaact tccttcaatc
     4201 ataagaactg tatcgactac aaagatgaca ttacttggag aaattaatcg aggtactttt
     4261 ggaggtgtag ttgcaacaga agaaatgggc gtaaatttct ttaaaaataa tcctaatatt
     4321 aatataacag gaagagggac tctaatatca attgcagata caggaataga ctatctacac
     4381 ccagatttta tttatccaga tggcacttca aaaatagtat atttatggga ccaaacaaaa
     4441 gaaggaactc ctcctgatgg tttttatatt gggacagaat atacacgaga agatataaat
     4501 cgagctatcg cagaaaatga ccctagtcta tctcaggatg aagttggtca aggaactatg
     4561 ttgagtggga tatgtgcagg gcttggaaat gtaaatagtg aatatgcagg tatagcagaa
     4621 gattctgagc taataataat aaaactcggc aagatagatg atttttataa tagtgcaatg
     4681 ctgtttgctg catctcagta tgcatataaa aaagcttttg aacttggaag accattagtt
     4741 ataaatatgt ctttaggtac cagtagccta gcaggtttaa ccaataggag taatagtgaa
     4801 aaagcgttct ttactagagg gttatgtata acggcaggag caggaaatga gggcaataca
     4861 cagacacata catctggaat aataccatat gttggaggtt ctgttgaagt tgaattagag
     4921 ttaaatgaag atgaggaaga attatcatta gaactttggt taaatagacc agataaagct
     4981 gatgtaataa tagtatctcc cactggtgaa gaaagtaaaa gtgttggaat ttcaaattat
     5041 aataaagtaa caggtttatt tgatttagag gggactgaat attctataac atatatatac
     5101 ccaacaactt tttctggaca gcaatttact aatgttactt taaagaatgc aaaaagaggt
     5161 gtatggaaga ttcgattagt aggagtttat attataacag gaagatataa cttatacctt
     5221 ccaaatagag aacttttaaa aagtggaact agatttagag aggtagaccc tttttacact
     5281 ataaattatc ctgcgattca agatgattta ataaccatag gagcctacaa tactattaat
     5341 ggtagtttat ggcaaagttc atcaagaggg ccaactattg aagacaggtt aaaaccagat
     5401 atagtagcac caggtgttaa tataatagct gcttatcctg ggaacacgta tgcaactata
     5461 actggtacag cagcagcaag tgcacatgca gctggtgctg ctgcaatgta ttttcaatat
     5521 acttttgttg acggtagata tccaaaccaa gcatatgtgc aaaaaataaa aacttttatg
     5581 caagctggtg ctagaaaaga ttcaaacaca gtatatccaa atacaaattc tggatatggg
     5641 ctcttggatg ttagaggaat gtttgatgtt ttaagatagg agggatttat ggaaaaatct
     5701 tattgtataa tttatcaagg tgatatagaa tctgccctac aagaaaatgg aataaatagg
     5761 tatatggttt taaatagtca gcttgcagta atatatgtac ctttagattt tgatgaaact
     5821 attttaaata atataataca agttgcttgg tgggaagaat ctgaaccaat gagcagtcta
     5881 attgaaataa ctaataatgt aaataatgga gaaaccatta caacagcagc tgaaactgat
     5941 tatatttatg aaaatccata taatgatata acagggagag gaattttatt agcagttata
     6001 gattctggga tagattattt gcatcctgat tttataaatg atgatggaac tagtaaagtt
     6061 ttatatttat gggaccaaga agcaaataca aatccacctc cagaaggttt tatttttgga
     6121 agtgaattta caagaagtga gttaaatata gccataaata gaaatgatgg gagtttgagt
     6181 caagataata taggtactgg aactttagtt tctggtatat tagttggaaa tggaaggata
     6241 aattctcagt acagaggtat tactacagaa tcagacttaa tagtagttaa attaaaatca
     6301 tatacagata cttattatgc tggaaggata aattacagtg tttcagattt tttagcagct
     6361 ataacgtatg ttactaacat agcaagaaca gaaaataaac cactgataat aaatttgact
     6421 attggagtta agtcaagtgc tgtagcaact acttcaatac tagatacttt taatatattg
     6481 agtagtgctg gtgtggtagt agtgagtggt gcaggaaatc aagggaatac agatattcat
     6541 tattctggca gatttagcag tgtgggagaa gttcaagatg taataataca agatggagat
     6601 gactatgctc ttgatataac actaaatacg aatggtccag ataaagtggg agcacagata
     6661 atatctcctt caggtgaagt tagtcatgat ataaggtatt ctccagactt ttatatatat
     6721 agaggaaaat ttaacttaga aaatactact tatgcaatga gatttattta tccatatatt
     6781 acctctggaa aagaaaattt ggagataaga ttaagagata taaaacctgg agtatggatt
     6841 ttaagattga cttcagaact tataataaat ggagaatatg acatatactt gccaaataaa
     6901 aatttgatag ctccagacac aaggttttta gaccctgatt cagtagctac tataactatg
     6961 tatgctgcta gtgatgatgt aattacagtt ggtacattta acaataagac tgatagtatg
     7021 tggattggtt cttcgaaagg accaattaga ggaagaggaa ttaagccaga tatagtagct
     7081 tcaggtgtag atataatatc cacatataaa aatggaacat ataatacagg aacaggaaca
     7141 ggagtgagca gttctatagt cacaggtgtt ttagccttat tgatggaata cttagaaaaa
     7201 caggataatg taccgagatt atcattgttt acacaagttt taaaaacata tttaatattg
     7261 ggtgctacaa aacttgagat atatacatat ccaaatgtat cgcagggata tgggatttta
     7321 aatttaaaaa atacgattca acagatagca aatactctat agataagaac ctatgtaaaa
     7381 ttataattga ttattagttt aattgtatca aatactttag atagagtaga aaaatagcat
     7441 aaaaaataaa tatgaaaatt aaaaaacagt tttatatttt gaatacacaa agtaaatatt
     7501 ttgtatataa ataggtataa attaagtatt gaaaagatat gatgttaatt taaaaataat
     7561 tataatttag acttatgaat tttatttgta aaattgctaa aactattaca aaataaataa
     7621 tagaggtgat agttttggaa agtaaaaagg caagagcatt aaaaggtaaa gataataaaa
     7681 gacttaaaag ttcactttta gttggaaatg agtcgacagc agcatgggct gatgttgaaa
     7741 aattaaaacc tcatagtaaa gtttcaatac cttcaattaa gggtgtcgaa gaagcaaaag
     7801 actgggttga caatggaagt aaattataaa attttggaaa ttgaagtaat aaagaagtaa
     7861 tgaagataag tatttattat cattatgtaa ggtagaatta ttatatttta aataacaatc
     7921 agtatcaatt atataaattt attgactaaa aatttataaa ttggtataat tttgttaatt
     7981 agcagaaata ataaaagata aatactgtga agagaagagt agatataaaa tgtagttcta
     8041 gcgagttgag gatggtgaaa gctcaatacg aggtttatat tgaagaacat cttggagttt
     8101 ctaatcgaaa gcttatttag taagtagact tagacggaag gcaccgttat aggctttaag
     8161 cattgcttaa ctaagaggtc attatgacaa ctagggtggt accgcgagag taatagtctt
     8221 tcgtcccttt atatagggat gaagggcttt ttttattgaa attatattta aaagaaatta
     8281 ataacaatta tactaggagg atttaaaatg caaaaggaat ttataacagt aaaagaattg
     8341 tacagagaaa aagaacagta catagggaag actgtaaaag tagctggatg gataagaaca
     8401 tctagagtat ctaaaaattt tggatttata gagctaaatg atggaagctt ctttaaaaat
     8461 atgcagatag tatttgatga gaaagtagaa aattttaaag aggtaggaaa attagcaata
     8521 agctcatcta tacttgtaga gggagagtta gtatctacag aaggggctaa acaaccagtc
     8581 gaaatacatg caaaaagcat agttgtagaa ggggaatcag atagttcata tccactacaa
     8641 aagaagagac atacgcttga atatttaaga acgatagcac atttaagacc aagaagtaat
     8701 actttttcag ctgtatttag agtaagatca atagctgcct atgcaataca caaattcttc
     8761 caagaaagaa actttgtata tgcacattct ccaatcataa caggtagtga ctgtgaaggt
     8821 gcaggagaga tgtttagaat aacaactatg gatttaaatg atattccaaa aactgaagat
     8881 ggcaaaatag actatactca agatttcttt ggaaaagagg ctaatttaac agttagtggt
     8941 caattaaatg ccgagataat ggcactttct tttagaaacg tttatacttt tggacctaca
     9001 tttagagctg aaaactctta tacaggaaga catgcttcag aattttggat gatagagcca
     9061 gaaatagttt ttgcagacct tgaagataac atggaacttg cagaagatat gataaaatat
     9121 gttataagct atgtattaga aaatgctcct gaagaaatgg aattttttaa tagttttata
     9181 gataaaggat tgttagaaag gttaaataag atagtaaact ctgagtttat tagaataacg
     9241 tatactaaag cagtggaatt attaattgaa tctgggcatg aatttgagta tcctgttgag
     9301 tggggatgtg accttcaaac tgagcatgaa agatatataa cggagcaaat atataattgt
     9361 ccagtgtttg ttacagatta tccaaaagat ataaaagctt tctatatgag aatgaatgaa
     9421 gatggtaaaa ctgttagagc aatggattta ctagttcctg gagttggaga aatcgtagga
     9481 ggttctcaaa gagaagaaag agaagatgta ttgttagata gaattaatga aatgggatta
     9541 agagaagaag attattggtg gtatttagaa cttagaaagt ttggtactgc tactcactct
     9601 ggatttggtc ttggatttga aagaataatt atgtatatga caggtatgtc taacataaga
     9661 gacgttatac ctttcccaag aactcctaaa aatgctgaat tttaataatt atatataata
     9721 aaacttatat aactttaagt ataaacacta aaatgacttg catatttatc tattttagag
     9781 atatgcaagt tatttttatt gtactataaa tatataattt gattactttt attttgcgat
     9841 ataatactta taggtttatg taatagtatg taacctttta ataaaatgca acacaattac
     9901 gtccctgttc ttttgcagta tatagagcct tatcacaatt acttataaac tcatttaatg
     9961 ttattgtctt tccaattttc tttgtataaa ctccaatact aacagttaca cattttaaag
    10021 gaacatcatc acgctcaaaa ttgcaggact ctataatttt tcgtaaacat tctgatttaa
    10081 gtttagcatc tgcctcatta cagttaaaaa tacatataaa aaattcctca ccaccatatc
    10141 gtatagcatg ttcattttca gaacttgtaa aatctaaaat tactttggca atattttgta
    10201 gacaatagtc tcccttttga tgtccataat tatcattaaa ttgtttaaaa taatctatat
    10261 ctatcatcat tccagttata ggttcttgat ttgcatggct gattaatttt ttttgtagaa
    10321 tatcttcaag gtatctacgg ttgttcattt tagtaaggtc atctgtcata actttgtcgt
    10381 taagtaaatt atttatatga gatatttctt catattgttg actaattaaa agtttatcat
    10441 agtaagtaaa tacacgattt ctatatagca ttgtagaaac aaaaatggca agggatgtta
    10501 taaaaaatgt gttgacaata ttactaaact cattgcctgg tatagtttga atcccaggta
    10561 agaataaatt aagaaacaaa aagcatcctc caaaaactat tactgattct ccaaatttta
    10621 atactgagaa agctgcagct gctaacataa tataactaaa tacacttagg tcatttccac
    10681 ctaactggtc aagtagagta attacacaac cccataggca gaaaaaagtc acataaatta
    10741 tccataaatt tatataaaac gtacagcaat cctttttttt attccaaagg taagaaaata
    10801 caaacataaa gacagaagat acacaaaata gtgttacata catataaaaa taccattggc
    10861 gtcttggtgt gacaaaaggg cctccttctc tagctgagat tgatatcatc ataattattt
    10921 gagatattac tattattatg caaatgattt tacccgtatg ataaagataa ttgcagagtt
    10981 ctttctcaac attgtctttg tgttttttgt ctagaggaaa tccaaaaaat ttttttattt
    11041 tttctaagtt catataagcc tccattaaaa ataaagcata aaaataagta ttcctttcta
    11101 tcattagata tttggaatac ttcgtttatg atttaattat atcaaaatat attgtgtgta
    11161 acaatctaaa attgtgatag ataaaattta ttagataatg tcgagtaact ttattgatta
    11221 atactttatt ataattaaga tttacagata gtttactact gaaaataaag aatagaataa
    11281 atttaaatta agcataataa aatataaata ataaagttcg cattaacaaa ctatttaaat
    11341 atcaagacct gttaaaaaac aacagcaata aacaaataaa atagataaat atatccaatt
    11401 aaggtataat taagttttat atgatatagt tatatttgat aatttacaat agtaaaactt
    11461 ggagggaaaa aatgggtgaa aagaaaataa ataatatgaa ttattcacat aataattatt
    11521 ccaaaaagac aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaagta
    11581 aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta
    11641 aattttgtgg aaatgaactt tatgaaatag catcattaag accacttgaa acaaaactag
    11701 acttgaaatc taaaataaaa gagctttcat atcatgcaaa taaaagaggt gtatttttaa
    11761 caacatttac aaccatattt atattgttta ttattgcatt gatatttaaa gctattataa
    11821 ctattcaatt taatgatatt agttatttag taaatccagc acatataatc ctagctctca
    11881 acttgggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca
    11941 atataggttt gttgatatta cttataatcc ctattttggt attattaata tcaaacctaa
    12001 tttttatgag aaaaagatgt agagattcaa aaactgtatt agcaaattct ctaggagttg
    12061 gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt
    12121 cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgttc
    12181 ttttaaatgg attcgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt
    12241 atgaaaaaga aaatatgtat ttatctcttt tttcaaattc tataagaaca ttcatattag
    12301 gttatgtttt agtattagta atactattgg tactaactat atctgatagt agttacttaa
    12361 atgaactaga tatgagtagt tattcaaatg gattaaattt atttactata ctcccacaaa
    12421 tagctagtta tatgtgggct tttgcaaatg gaatttcagt aaccataata aactcaacag
    12481 tatcaatgtt tactttaagt tcttcatctt tatttggaga tactaaatta atgttttatg
    12541 caatgggagc attgtctatg ttaatattat tattaaatgg atacaagcta agatttaagt
    12601 ataatacaga tagtataaga cctataatag tatttagtat atattatgca tttttgatgg
    12661 gaatattggc cttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa
    12721 ctaattatgg aacaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat
    12781 ttgtatatag ttttgtgata tctttaatag gatataagct aaattcagct gattaaatat
    12841 gattggaggg aatttctgtg gaaggaaata ctaataataa agtagatgat aaattttcta
    12901 aggttaagaa aaagtttggt agtggaaaat ttaaagttga attatataaa gaaaaatcaa
    12961 aattaaatag agagattgat gatattcaag aaaagaaagc aaagatattc ttggaaatgg
    13021 gaatactgac atatcaaaaa attagagaag attctattaa tgataatagt tttgataaat
    13081 tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg
    13141 aaatggaaat ggcagaaagt aatgtaactt gtgagtgtgg ttatgttgga aatgtaaatg
    13201 ataaatattg tgctgaatgt ggtaaaaaat ttgaattgga aaatgaatgt gatgatttta
    13261 taatttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgctgtg
    13321 gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg
    13381 tttttttatt gaaattattt aaatggactt ataacagtag ttatataaaa tgaaaataaa
    13441 caccaaaaaa atgaaataaa atttctttta aatgtaaaaa acaagctaat atataaaata
    13501 aatcagtaaa aatatagatg cgagaaaaaa tatgaaaaaa tattgcaaag aaaaagtttt
    13561 catgttatta ttaatacata aatcaagaaa ataaatttca gaaacgaggt aatttaaaaa
    13621 atgctagata aggtaaaggg aagattaata gtatcatgcc aagctttaga aaatgaacct
    13681 cttcatagtc cttttataat ggggagaatg gctaaggctg ctatggaagg tggagctgtt
    13741 ggaattagag ctcaaggagt tgaagatatt attgaaataa aaaaagttac tggacttcct
    13801 gttataggta ttataaagag aaactatgaa gattcagata tatatataac gccaactaaa
    13861 aaagaagttg atgaactttt aacaacaggg tgtgaaatga tagcattaga tgctaccaat
    13921 agagttagac caaataatga agacttaaaa gaacttataa agtatataaa agaaaacggt
    13981 gttctagtaa tggcagatat ttcaaattat gatgaggcta tcaaagcaca agagtacggt
    14041 gtagattgcg tttcaactac actatctgga tatactccat atactaaaac attagaaggt
    14101 ccagattttg tattaatgaa aagattagtt aaagatttag aaatacctgt aatagctgaa
    14161 ggaaaggtaa atactcctca agatttaaaa aaggtctttg agttgggtgt tcattcatca
    14221 gtagtaggtt cagcaataac aagaccacaa ttaataacag aaaaatttgt taaagcaatt
    14281 gaaattaatt aaaaattaat ttataaatta aaaaattatt atatttaact atatttgaaa
    14341 attaaaattg gaggtaaaga tatgagaaca acagatatga agggaattta ttcagcatta
    14401 ttagtttcat ttgataagga aggaaatata aatgaaaaag ggttaagaca aataattaga
    14461 cacaatatag atgtttgtaa agtagatggt ctttatgtag gaggaagtac aggggaaaac
    14521 ttcatgcttt caactgatga aaagaaaaga atatttgaaa tagctaaaga tgaagtgaaa
    14581 gaagaaataa aattaatagc tcaagttggt tcagttaatt taaaagaagc tgtagaactt
    14641 gctaaattta caactgattt aggatatgat gcaataagtg ctgttacacc tttttactat
    14701 aaatttgatt ttgaagaaat aaaacactat tataatacaa taataaattc tgtagataat
    14761 agattaataa tatactctat accattttta actggtgttg atatgagttt agaccagttt
    14821 ggagaattat ttgaaaatga aaaaataata ggtgttaagt ttacagcagc agatttctac
    14881 ttattagaga gaatgagaaa aacattccca aataaattaa tatttgcagg atttgatgaa
    14941 atgatgttac cagctacagt tttaggtgta gatggggcaa ttggttctac attcaatgta
    15001 aacggtgtca gagctagaca aatattcgaa ttaactaaga atgaaaaaat atcagaagca
    15061 cttgaagtac aacatgtgac taatgattta ataacagata tattaggaaa tggcttatat
    15121 caaacaatta aattactact tgaagaacaa ggtgttgagg ctggatactg tagacaacct
    15181 atgaaagaag caactgatga aatgaaatca agagcaaaag aaatatacag aaagtatttt
    15241 taataaaata cttttagtat gggaggatta gtcaaatggt aggatttact tggatagatt
    15301 ttgcaatact agtggtttac ttattggcag ttttatttgc aggattgtta ttttcaaaaa
    15361 aggaaatgaa gggaaaagag ttcttcaaag gtgatggaac tattccgtgg tgggttacat
    15421 cagtatcaat atttgccaca ttattaagtc caatatcatt tttatcatta gcaggaaatt
    15481 cttatggtgg tacttggatt ttgtggtttg cacaattagg tatgtttata gcaatacctt
    15541 tgacaataaa atttttctta cctctatata gtaggttaga aatagataca gcatatgaat
    15601 acttagaaat gagatacgaa agtaaaggtc ttagagtact tggagctttg atgtttataa
    15661 tatatcaaat aggacgtatg tctattataa tgtatcttcc atcaatagtt ctggctggtc
    15721 ttacaggaat acctgtaaat gtactgataa tagttatggg tgtaatagca ataatttact
    15781 catatacagg tggattaaag gctgtcttgt ggaccgattt tatacaagga atggtattaa
    15841 tagtaggtgt tacagggaca ttgttctatt taatagcaag tataaatggt ggttttggaa
    15901 cagtaatgga tactcttaca actggacaca agttcttagc aagtaatgaa gttatgtttg
    15961 ataaaaatat actatctaca agtgcattta taatatttgt tggagctggt cttaatactt
    16021 tttcatcata tgtatcgagt caagatatag ttcaaaggtt tactacaaca actgatataa
    16081 agcaacttaa taaaatgaca tatggaaatg gtgttttatc aatgggactt gcaacagtat
    16141 tttatctaat tggaacttgt ttatttatat actatactca aaacccagat ttagctcaaa
    16201 cagttcaaca agaccaagta tttgcatcat atatagctta tgaattacca gtgggtataa
    16261 caggaatact acttgcagcg atatatgcag cttctcaatc aactttatcg acagggctta
    16321 attcagttgc tacaagttgg actttagata tacaaactat cataacaaaa gatataagta
    16381 tggaaaagca aactaagata gcacaatata tatcattagg tgtaggtata ctttctatag
    16441 ctgttgcaat agtattagca aatggagaaa taaagtcagc atatgaatgg ttcaatagtt
    16501 ttatggggct agtactaggt gttttagcag gaatatttgt acttggtgca ttttgtaaaa
    16561 aagcaactaa aatgggagca tatgttggat tcatagtatc agcaatttta gttgtttatt
    16621 taaaataccg tatgccagaa gtaacttctt gggcatattc tctaataaca atcacgactt
    16681 ctgtagtggt aggtacaata gttagtgcaa tagaagctaa agttaaagca aaagtatctt
    16741 taccaaaagc tgagactact gtttattatg aaaaataata tttaaatgat aaattgtctt
    16801 atagagtgat tagataaata atttagttac tctataagat tgtataatat actttgtata
    16861 caaaagtata aaaatagact ttataattat atagggagat agtgcttatg atatttggaa
    16921 atataaatca tagtaagaca tattcaacat tgcatgaaga tttacttaga tgttttgaat
    16981 atgctaaaga taattcatta atagattatg aaaaaggtac ttatacaata gatggtgatg
    17041 atatttttgt gaatattgta gagtataaaa cttgtgaaaa agaagataga ttctgggaag
    17101 ctcataagaa gtatatagat gttcatttga tgcttgatgg atgtgaacga atagatataa
    17161 attttataga aaatttagaa caaaaaatat atgaagaaaa gggcgatttc ttgcctcttg
    17221 atggtaaaaa taatggatac atggaactta gaaaaggtga ttttttagtt tgttatccgg
    17281 aagatgctca tatgacagga ataaaagttt tagaaaaaga aaatatcaaa aaagctattt
    17341 ttaaggtttt ggttagataa tgataattta tataaccact agggctgaaa atagtgattt
    17401 aactttcagc gattagtgag acttcgtaaa ttttagttag attactattt tcagccttag
    17461 ttttttatga gaaaattaaa aataattaaa agcttaaaat tttgtaaagg agttaactta
    17521 tggaaaatat agatgtattc ttattaatag gacaaagcaa tgctagagga ttgggaaatc
    17581 caaaagagag catcattcca aatgaaaatt gttttgaata tttgagtact gatgaaatta
    17641 taaatatgag atgtgagcta gagacttcag aaggtgatgg tactatagca cctgcatttt
    17701 caaatgaatg gaataagctt actggaaata aagtttgttt tattcacaat gcaaaagatg
    17761 gttcaagaat aaaaaactgg aatcatgata ataactggtt tctaaatgac actatagaaa
    17821 aatttaatac aggctgtact accttaagta aacattataa tatagaaaat aaatacgtta
    17881 tttggattca aggtgaaagt gatgcaaaat atggtagtga tgtattgtac tataaagaaa
    17941 gtctaaaaaa aattgcgtat aggcttaaag aagaatgtat gattgataaa atgtttgtaa
    18001 gtttaactgg ttattggctt ggagaagatg aatattttat tagaacaaga cgaattgcag
    18061 cagcacagga gtcggcttgt agtgaatgtg atatattatg tgtaggaagc aaaattgcta
    18121 tgcagtttca tgacagagga ttaactattg atgatgttca ttacactcaa gaaggtctta
    18181 atatgttagg agaggactta tgtaaaaata tttataagta tcatataacc aaagaaaaaa
    18241 gtgtattaag tgatactata gacttatcag aagcaaggaa atatatctgt gaattggaaa
    18301 agataagtaa aagatttgtg taatataaaa ttttaaatag agttgttaat ttataaaaat
    18361 aatttgtatg gagatatgtt atgaaaaatt acgtttgtat agatgttgga ggtacttcaa
    18421 taaaatatgg ttttattaga gaagatggat ttataattga taaatcaagt cttgatactg
    18481 aagcaaagga aaaaggtgga gaaggaatat tagccaaaat aaaagatata gtaaaaaaat
    18541 atatagagga aaatgaaata agtggtatat gtatatcaac tgcaggtatg gttgacccag
    18601 ttgaaggtaa aatactattt gcattagaag aattaattcc aaactataag gacatgcaat
    18661 taaaaaaaga agtagaaaaa gagtttaata taaaatgtga agttgaaaat gatgttaatt
    18721 gtgctggact tggtgaaatg tggcttggag caggtagagg tgcaacatct tctatatgtt
    18781 taacaatagg tactggaatt ggtggatgta ttataataaa taataaactg ataaatgggt
    18841 ttagtaatag tgctggtgaa gtaggatata tgaatgtaaa tggaagttct tttcaagaat
    18901 tggcatctac tagcagtcta attaaaaaag tagcaaaaat aaaaaatttg aatgaaaatg
    18961 acttaaatgg aaaaataatt tttgatttag caaagaataa tgatgaagat tgtttaaaag
    19021 aactcgataa tatgataaaa tcattagcag ttggaatcgc aaatatatgt tatgtaataa
    19081 atccagaagt ggtaatttta ggtggtggga ttatggcaca agaaaagttc ctaaaaccaa
    19141 aaatagacaa agcattaaag gaggttctta tagagagagt ttacaaaaac actaatatag
    19201 aatttgcaaa gagacaaaat gatgcaggaa tgcttggagc attatataat tttttaaaca
    19261 aaatataaag aatagaaggg tttttaatga gtattttaga acaattggaa tcaccaactt
    19321 ttaaagtcac aaaatcagat aaattattaa tagcttatat aaaagaaaat atagatgatg
    19381 tattttataa accaatatca caaatagcta aagagagcaa tattggtgaa gcaactataa
    19441 ctaggtttgt aaaaaaaatg aattttaatg gattacagga ttttaaagtt actttagcac
    19501 aagaaatatc aactatcaat aaaaaaaata ttataaataa aaatatacaa aatgatgagc
    19561 cagctttgga tacagcaaaa aaacttttaa gttcaaatgt aaccacttta gagaacacag
    19621 tagagataat caatagtaag gatgtacatg actgcgcaag gcttataata aatgcaaaga
    19681 aagtttactt cataggtatt ggatattctg gtataatagc acaggattca aactataaat
    19741 ttatgagaat aggtcttaat tgtgtaagtt ttgacagtag tcatacaatg ataatgatgt
    19801 cttccataat ggaagaagga gatttaataa ttgcaatttc acattctggt gaaacagagg
    19861 agattataaa aactgtaaag cttgcaagag ccaataatgc taaaattata tcaattactg
    19921 aaaataaaaa ttctgaatta aaggatattt cagatgtaca cttatcttat gtatcgggag
    19981 aaacagtact ggaaaccggt tcaatatctt ctaaattagc acaatttttc attattgatt
    20041 tagtatatac acaggtagta aaagaacttt ctaatgaagc tattgaaagg aaaattaaaa
    20101 ctacaaatgc tataaaattg tttaaaaatg aataaaataa gaaatttgta atgttttata
    20161 gagctctctt aaaaatttag gaatggagag atattacatg tctaaattaa aagattataa
    20221 tattgataaa gtaataattt ttaataattt aagtactaaa acgaagaatc aacttaaaga
    20281 aaaagttaaa ctaaaaagat tacaaaaaaa agaaatttta ttttatgaaa aagatagctt
    20341 agacagtgtt tatatacttt tagaaggaaa ggtatctatt tttaagataa gtgaaaatgg
    20401 agagagaaaa gtaattttca tattaaatag tggggaagtc ataaatgata ttacacttga
    20461 cgcttctaaa aactctactg ttggctgtga agcatttgaa aattcaattg tagcttatta
    20521 tagtattgaa gaatttttag aaattatgca aaatgatttt caacttacaa aggatattat
    20581 ttcttatatg gaaagaagaa taagacgttt atatagacag ttgaaaaatt ctatatctat
    20641 aaaagtagat aaaaaacttg cagcaaaatt atataggtta agtagagaat ttggagtttg
    20701 ctgtggagaa tggactttac taaatgtaaa tattacagtt acatatttag ctgatatgct
    20761 tggctgtaaa agggagactg tttccagaat gataaaggta ttgcaaaaag aagaacttat
    20821 taaaattgac aataaaggtt tttatgttaa agaaactgaa ttatcaaggt attttaaaaa
    20881 taattaatta tgattaagta agagtattat tagaatttta attaaataaa aatattatta
    20941 agagaatcta tttatagatt cttttttatt gtctaattta ttaattttta ttttacccta
    21001 ttgaattgta taaaaaaaca aataagattc ttttttattc aaaaaactta ctaaattttt
    21061 gattatcttt tcataagttg gtaaatgccg atatatcccg tatttgaggg ctttatcggt
    21121 attttctttt tggaagatac cttgcataag ctggcattta ccagcatgaa aatgcaacca
    21181 aaagtagtaa atgagtagta aatatacgtt ccgatattaa caagccagtc tttccagttc
    21241 tgcttttgca gattggaatg taccgtgtgc gtagtagcca agcgtcatgg ttatgttagc
    21301 gtgtcccatg agatattgca gggtgttcgg gttcattccc ttgtttgcca tattcgtaca
    21361 ataggtatgt cggaatgaat gtggtgtaat gtttggtaac ttgtctttgt ggtatttgtt
    21421 atacttctta atcagtcctc gtaccatacc cacatagttt ccagcgactt taggcaatcc
    21481 ctcacggttt aagaatagga aattgctgta accaccaaca accagcggtt gagcttttac
    21541 tctgttcttt agtattcttt ggagtgcttg ataagccctt tccgtcaatg gaatttttcg
    21601 ttttccgttc tttgtttttg gagtgtcaat atagtagccg atttcgctat ctcttaatag
    21661 ctggtggtct atattgagta ttctgttctg catatcaata tgcgttgtca gtccgcaaaa
    21721 ttcagaaata cgaagtcccg tttccagaag aagcacaacc tcatcataat acttgctgta
    21781 aatcttatcc gtttccataa aggcaagcag gcgttcttcc tgctctggcg taagaataac
    21841 tttcggctcg gtatcatctt ccagaacatc acttagctta aattcaaacg ggttctttct
    21901 gatacagtcg tcttgtatcg ccatataaaa tgacgctttc aaggaacgct tatagttgtc
    21961 aatcgttttg taggcatatc ctctttcact cattctgata gcccactctt tagcgtccga
    22021 ctgtttgacg gtatcaatcg ctctcatacc caatgggtca tttttcagag cgttcataag
    22081 atactgtcgt cctttttccg tagctcgctt gatgttcttt ctttggctgt tcttcttgtc
    22141 gtaaagctgg cagactgtca ttttaccgcc tgccgtgtca ataccgtcat caaggtcttt
    22201 ttttatctgt gcttcttttt ccctcaacga tatatcatca cgttttccag caggcgtttt
    22261 gtctgtcggt acaagtttcc aagcataaat aaactgtggc tttccgtata tatcggtata
    22321 tttataaacg tatcttccgt cttttcgctg gctctctcca ttacgcaaat tgcgattttt
    22381 actgtctttt cgtttcacat tagacatagt gtaaagctcc tttccgtcat ggaatgagcc
    22441 gtgatacgct atacttatta taccatatcc acggctctat gacattagat ttcgtccaat
    22501 gtatctatga ttttttcaaa ttgttttcgc tttatctgaa tacggttgcc gttcacgata
    22561 acccagccag cgtcaatatt ttcttcggct aatttacgca acttcttttc tccaatgcgg
    22621 aagtatttgg acgcttcttc aatcgtcagt gtatattttt cccagatagg cacatcagta
    22681 ttattcataa tttatgcacc ccctttactg tgtgtcagtc tataagttat ataaacaggc
    22741 agacggcttt ggaaagctcc attagtattt caactattcc cattcttgtg ggcagaaccg
    22801 caccatgcgg aagtatcatt atgctgtaag ggtaggtcat ggcaaaacga cttttccaac
    22861 ggtcgctctc ggatcactgc gtgggtcgct cgctttcttt tggaaaggtc atggcgtaca
    22921 gttcccgtgg ctctctatcc ttacaaacgt atctgtctgc tttattcagt tttcaaagag
    22981 ctgtggcgaa agcggtatgc tttcatgtaa aagcaggagc agagcaggaa agaggggatt
    23041 gaacaaatca tgctctgcgt tactgctggt tattcttctt caatttctaa ggcaatatca
    23101 aaccctaaaa tcattttgat aagtgcttct cggataagtc cttttaactc catatctaca
    23161 acgatataaa cattaccgta ttcatcatag agtggacgca aacagcactt cgatatgtaa
    23221 gggtcataaa atgcaagtac cttttcaatc gctttttcgt ccccgtccat agcttgtgaa
    23281 agcaagtagt aagacggctt cttaaatgtg tgtttcattt gctatcgttt ctcctttaat
    23341 agttttctca taagtgccaa tgaattgtgg cggttctgaa aaactccgct tctggaaatg
    23401 ttttgctttt tggcgatttc ttcattgttc atttccaaaa agtaatacat caacaaagtt
    23461 tctcggttac gttcggataa ctgtttcaag gcttctgcca attcatcatc atagacacga
    23521 atatcattac cgcatacatt gaaaatcgta tagtctgttt cgtagtcgtc ccatacagcc
    23581 aagttttcaa ccatgatttc tggaagttca caaaaggaaa tttctttttc ttttcttcgc
    23641 ttcaatccct gctgatagtc cttgacaaca tgacggacaa ctttcttcaa acagctttca
    23701 aaacggcatt gaacagtttt ctggaagtca gacggtttca tcaatctcac cccctttccg
    23761 ttatgacgtg aaaagtgttt atccctcttt ccgcaccata tagggaatgg caagtgtgat
    23821 ttgttaagtt gaaaccagaa aaaatcgaaa aaacttttta gtaaaacaaa aacagcccgt
    23881 gtgaatgtga atacacaaac ggactgcttt ataaatgcta tctgtatgaa tttttaactc
    23941 ttgtagaggg tagaatgatt gtaatactga actaaaatat tttttcagtt gggaacagca
    24001 aagtattatt ccatatacaa tcatggcgta gcccccttta ccagagctat accttgtgcc
    24061 accaatgaaa aaggacggct ctggctatat ccaaaaccgc cccgttatgt tttcaagacg
    24121 cacaaaagta tatcatctcg gaacggttat ggtgatttaa aatgagaaaa acaaaagaaa
    24181 cacatacttt tgatttcaga ccgctagggc tggcaatcag agaagcccgt gaaaaagctg
    24241 ggctttctcg taatgattta ggcgataagg tattctatgg ggaa
//
LOCUS       DAMJVI010000054        24059 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.056, whole genome shotgun sequence.
ACCESSION   DAMJVI010000054 DAMJVI010000000
VERSION     DAMJVI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24059
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.056"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            214..1764
                     /locus_tag="PC545_002212"
     CDS             214..1764
                     /locus_tag="PC545_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2456979.1"
                     /translation="MRYKKRNIDKSSELSFQSLAKYFITFYIVIILIILSMFCINTIW
                     VMKKDNQDLLSTTSNRIEDRVGETIKLLNSLSKDNYIIDNSVNGIDKSKELSMYSDEF
                     GYMMICFVDKNIIVWDEEGGNISLAGRDYMQKLFATKKYQVTDSFAAGADGKTLNYTI
                     ASPIIKNGNVDGCVFAAIYFDEIKDILKQGKGQQQQNFILFGSENQIMNLTSDETAYG
                     EKYMKLSQKTKYLGTNAKKVEKDMFNNKSGDYWAFENGKLYFMSYKPIKNTNWSLFCK
                     VNFLDIFALNLIPMGAILSVITLVFIILICMEKSFMKKRMEVIDMLLGSVQELEKRIY
                     QTERPSEVDFKEIIQLTSKGLTDGLTGVVTRTVFFDQIYNRVEMEENDKLSVISFVDL
                     DDLKCINDLYGHEIGDMVLKNVGYVLREYEIKYNGLIGRYGGDEFVMLLSGFTSREEV
                     EDLLDEIAIRLHTEVLTDDGIIQTHCSVGAVVCSVKDNKDIDNLLKQADEALYYVKRN
                     GKGNYHLV"
     gene            1790..4492
                     /locus_tag="PC545_002213"
     CDS             1790..4492
                     /locus_tag="PC545_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2456980.1"
                     /translation="MKNVSKKFFVCTIIVLITFLCFSKFAIYVFDKQEKKETKVVENM
                     LNVYKNNIILNLTEKFNYSKDLTSVLVSNNYSIDKLKPIADEILKNNSCIYISVVEKY
                     NVAAFFSNSVKKDIINTPIQELNYSYSLANSIKGLVVEGPSKDQFSNGDVFLITNPIY
                     KNGEYWGEITVALDAKKVVDSFNLNKLKESGFEYELWRVHLDNGSKSTIEISTKDFNF
                     SKSHEIKFKLPTVWTLSIVYKNGWNNKNIDFIVFVILLIISILIGVMTFIYIEKKSIS
                     KELNQQSLKNLETGILNREGFLIKLQEHLNRKNNEIVLLYICITNYSKFAPVMSFEKR
                     QDYLTHIVKNLDNYIKSSHFIARISEEGFVLALEENCDKNELLNIEKGISLELLRKIK
                     VENKQVFLTIGYGSSRCVDDRKDAESLLLSAIEDYERRFPAMSPIHDFTKKCEDMLDE
                     KKQIVFGEYPDLEINKLSKVIYKYYKKVQRIMYKDYNIDIGNRRGYMRDVEVLIDYNS
                     ERMLYLFLIDICKFGKFNELFSIEIGDVILKEVSNKLKTIFSNYLYRINGDVFLGISI
                     DKESYLDTLHKVNEVFNSSLSVEDVLFDVKCNIGICQYPYHGENPYDLLEKVQIALEY
                     AKKDVKSNVKVYDKELTEEIYHENNIIKDLKKGLEEQTLEVWYQPIFNVSKNCYIKAE
                     ALIRLRNKEGGYYSAYEVITYAEKHGLIKDVGLYVLKKACDVTTKIKDKNIDFEQMQL
                     NLSAQQIVHKQCANDILSCIENCGLDKKYISVEVTEKVLMETFEIVNVTLDKLKKEGI
                     SIALDEFGSGFSSINYLANLPIENLKIDKELVEQITYSKKQFELLKTIVQITKINGMD
                     IVAEGVETKETLDLVVTSGVNLIQGHYFSEPLSEDKFIELMIEE"
     gene            complement(4998..5663)
                     /locus_tag="PC545_002214"
     CDS             complement(4998..5663)
                     /locus_tag="PC545_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456981.1"
                     /translation="MKGNIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKINNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NDNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDDDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAG
                     NLN"
     gene            6007..7437
                     /locus_tag="PC545_002215"
     CDS             6007..7437
                     /locus_tag="PC545_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2456982.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDLKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKSLKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEKICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
     gene            7805..8383
                     /locus_tag="PC545_002216"
     CDS             7805..8383
                     /locus_tag="PC545_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDF2456983.1"
                     /translation="MFKRYAEKMTSVLICNNMIDNNESKVYSYGFEILIAFIVNITTM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENINI
                     DLFKNIIMIIASVSWVGICVLCPIEHRSNPISDREKLVYKKTAIFISTVVLLITILSL
                     SLSIFVDYFTYSAFAMFWIFVMLVLGKLKAKV"
     gene            8413..8559
                     /locus_tag="PC545_002217"
     CDS             8413..8559
                     /locus_tag="PC545_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HDF2456984.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKTN"
     gene            8864..10243
                     /gene="scfB"
                     /locus_tag="PC545_002218"
     CDS             8864..10243
                     /gene="scfB"
                     /locus_tag="PC545_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HDF2456985.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVDYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSVTPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            10272..10769
                     /locus_tag="PC545_002219"
     CDS             10272..10769
                     /locus_tag="PC545_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDF2456986.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKKYIDESYEWYLEAA
                     QNQKY"
     gene            11067..12731
                     /locus_tag="PC545_002220"
     CDS             11067..12731
                     /locus_tag="PC545_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDF2456987.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERMKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIDIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGVLKTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKVYSEYNTKKILVMEKVNGTKLSDVEKIRRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLIEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNVEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            12744..15194
                     /gene="recJ"
                     /locus_tag="PC545_002221"
     CDS             12744..15194
                     /gene="recJ"
                     /locus_tag="PC545_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDF2456988.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKLAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYNVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQSEIPSAFAVINPKQEDCNYPFDSLCGCGVAFKMIQALTPKEEFKTSMYN
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILEEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLGIENGEATGSARS
                     IKGFNIFEALIKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NVNVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     SIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDDSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRETFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            15207..15719
                     /locus_tag="PC545_002222"
     CDS             15207..15719
                     /locus_tag="PC545_002222"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDF2456989.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKDVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            15793..18000
                     /locus_tag="PC545_002223"
     CDS             15793..18000
                     /locus_tag="PC545_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDF2456990.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            18025..18474
                     /locus_tag="PC545_002224"
     CDS             18025..18474
                     /locus_tag="PC545_002224"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDF2456991.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAARPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            18489..19103
                     /locus_tag="PC545_002225"
     CDS             18489..19103
                     /locus_tag="PC545_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2456992.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHVDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGDFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            19194..20750
                     /locus_tag="PC545_002226"
     CDS             19194..20750
                     /locus_tag="PC545_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HDF2456993.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFKDSNANNNIESKHLIN
                     RLIYENGVLFSSTEYYENGNLKYESIQNIENMNLEFLDGFDSSKTIEEFLNSLDSESK
                     RNFKKLCKENIKKSMFLVLKQVFNSYVPWGILTGIRPVKIVHNLMDKKLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKVIKDTKKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIKEFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLEEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            20740..22002
                     /locus_tag="PC545_002227"
     CDS             20740..22002
                     /locus_tag="PC545_002227"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDF2456994.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            22076..23863
                     /gene="aspS"
                     /locus_tag="PC545_002228"
     CDS             22076..23863
                     /gene="aspS"
                     /locus_tag="PC545_002228"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDF2456995.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKEAFEKAEKLGAEFVIAVKGKVCERQSKNPNMLTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEAIFKEVLDVELALPL
                     PKMTYAEAMSKYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFNEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKSSLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGTDNIREVIAFPKNQNA
                     VCPMTNAPTLAEDEQLEELSIKVDIKDNE"
BASE COUNT         9663 a         2382 c         4322 g         7692 t
ORIGIN      
        1 ttacgtatca aaatttgtaa aatgaataga tataattact aaatatgtga tagtattggc
       61 tataatagga tataaaaaat aatattgtaa aaaaaagttt atatttagta atgaaaattt
      121 aacgttttac ttgaaattat gtgttttgta atgtattatt aaggctctaa taggaacatt
      181 ttcatcaatt tttctatagt gggggtagga attatgaggt acaaaaaaag aaatatagat
      241 aagtcaagtg aactttcatt tcaatctcta gcaaaatatt ttataacttt ctatatagta
      301 attatattaa ttattctttc tatgttttgt ataaatacaa tatgggttat gaaaaaagat
      361 aatcaagatt tattatctac aacatcaaat cgtattgaag atagggtagg agaaacaatt
      421 aagctattga actctttgag taaagataat tacataatag ataatagtgt gaatggcata
      481 gataaaagta aagaactttc aatgtatagt gatgaatttg ggtatatgat gatatgtttt
      541 gttgataaga atataattgt gtgggatgaa gaaggtggta atattagtct agctggtcgt
      601 gattatatgc agaaactttt tgcaacaaaa aaatatcaag ttacagatag ttttgcagca
      661 ggggctgatg gaaaaacttt aaactacact attgcatcac ctataataaa gaatggaaat
      721 gtagatggtt gtgtatttgc agcaatttac tttgatgaga taaaggatat actcaaacaa
      781 ggaaaaggtc aacagcaaca gaatttcata ttatttggaa gtgaaaatca aataatgaat
      841 ttaacaagtg atgaaactgc atatggagag aagtatatga agttatcgca gaaaacaaaa
      901 tatcttggaa ccaatgcaaa aaaagtagaa aaggatatgt ttaataataa atcaggtgat
      961 tattgggctt ttgaaaatgg aaaattatac tttatgtcct ataaaccaat taagaacaca
     1021 aattggagtt tattttgtaa agttaatttc ttagatattt ttgcattaaa tttaattcct
     1081 atgggagcta ttctatctgt tataacttta gtatttatta ttttgatttg tatggagaaa
     1141 agcttcatga aaaaacgaat ggaagttatt gatatgcttt taggttctgt tcaagaatta
     1201 gaaaaacgta tatatcaaac agaaagacct agtgaagtag actttaaaga aattattcag
     1261 ctcacaagta aaggtctgac tgatggatta actggtgttg taacacgtac tgtatttttt
     1321 gaccaaattt ataatcgtgt tgaaatggag gaaaatgata aattatctgt aatttcattt
     1381 gttgatttag atgatttgaa atgtattaac gacttatatg gacatgaaat tggggatatg
     1441 gttttgaaaa atgttggata tgtacttcgt gaatatgaga taaaatataa tggtttaatt
     1501 gggcgctatg gtggagatga atttgttatg ttgttatctg gttttacatc gagagaagag
     1561 gtggaggatt tattggatga gattgctatt cgcttacata ctgaagtact aacagatgat
     1621 ggtatcattc aaactcattg tagtgttgga gctgtagtat gtagtgtaaa agacaataaa
     1681 gatatagata atttattaaa acaggctgat gaagctttat actatgttaa acgtaatgga
     1741 aaaggtaatt atcatttagt ttaatgtatg tggaaggagt ttatatcata tgaaaaatgt
     1801 atctaaaaaa ttttttgtat gtactattat tgtattaata acatttttat gttttagtaa
     1861 atttgctatt tatgtttttg acaaacaaga aaagaaagaa acaaaagtag tagaaaacat
     1921 gttaaatgtc tacaagaata acattatttt aaatctgact gaaaaattta attatagcaa
     1981 agacttgaca tcagttttag tttcaaacaa ttattctata gataaattaa aacctattgc
     2041 agatgaaatt cttaaaaaca attcttgcat atatatttca gttgtagaaa aatataatgt
     2101 agctgcattt ttttctaatt ctgtcaaaaa agatattatc aatactccaa tacaggaact
     2161 gaattattct tattcattag caaattctat caaaggatta gtcgtagaag gtccttctaa
     2221 agaccagttt tcaaatggag atgtgttttt aattactaat cctatttata aaaatggaga
     2281 gtactgggga gaaattacag tagctttaga tgccaaaaaa gttgtagatt cttttaactt
     2341 aaataaatta aaagaatctg gatttgagta tgaactttgg cgtgtacacc tagataatgg
     2401 ttcaaaatct acaatagaaa tttctactaa agattttaat ttttccaaat cacatgaaat
     2461 taagtttaaa cttccgactg tttggactct tagcattgtg tacaaaaatg gatggaataa
     2521 taagaatata gattttattg tatttgttat attgttaatt atttctatat taataggtgt
     2581 gatgacattt atttacattg aaaagaagtc gattagtaag gaattaaatc aacagagtct
     2641 taaaaattta gaaactggta ttttgaatag agaaggtttt ttaattaagt tgcaagagca
     2701 tctgaataga aaaaataatg aaatagtgtt gctgtatata tgtattacta attatagtaa
     2761 atttgctcca gttatgagct ttgaaaaaag acaagattat cttacacata tagtaaaaaa
     2821 tctagataat tacataaaaa gttctcattt tattgctcgt ataagtgagg aaggttttgt
     2881 tctagcttta gaagaaaatt gtgataaaaa tgaattgctt aacattgaaa aagggatttc
     2941 cttagagctt ttacgaaaaa ttaaagtaga aaataaacaa gtatttttaa cgattggtta
     3001 tggctcatct aggtgtgttg atgatagaaa agatgcagaa tctctgttac tttcagctat
     3061 tgaagattat gagagaagat ttccagcaat gtctcctatt catgatttta ctaaaaagtg
     3121 tgaagatatg cttgatgaaa agaaacagat tgtatttggt gaatatccag atttagaaat
     3181 aaacaagttg tctaaagtaa tatacaaata ttataaaaaa gtacagcgta ttatgtataa
     3241 agattataat attgatattg gaaataggcg agggtatatg cgtgatgtcg aagtattaat
     3301 tgattataat tctgagagaa tgttatactt atttttaata gacatatgca agtttggaaa
     3361 atttaatgaa ctgttttcta ttgaaatagg agatgttatt ttaaaagaag ttagtaacaa
     3421 attaaaaaca attttttcta attatctata tcgtattaat ggagatgtat ttttaggcat
     3481 ttcaatagat aaagaatctt acttagatac tttacataag gttaatgaag tatttaatag
     3541 ttctttaagt gtagaagatg tactttttga tgtaaaatgt aatattggta tttgtcaata
     3601 tccatatcat ggtgaaaatc catatgacct tctggaaaag gtacaaattg cattggagta
     3661 tgcaaaaaag gatgtcaaaa gtaatgttaa agtttatgat aaggaattga cagaagagat
     3721 ttaccatgaa aataatatta ttaaggattt aaaaaaaggt ctggaagaac aaacactaga
     3781 agtttggtat cagccaattt ttaatgttag taaaaactgt tatataaaag ctgaggcact
     3841 aattagattg agaaataaag agggcggtta ttattctgcc tatgaagtta ttacttatgc
     3901 agaaaagcat ggacttatta aggatgtagg tctatatgta ctaaaaaagg cttgtgatgt
     3961 aactacaaaa ataaaagata aaaatataga ctttgaacaa atgcaattaa atttatctgc
     4021 tcagcagatt gtacataagc agtgtgcaaa tgacatatta tcatgtattg agaattgtgg
     4081 tctagataaa aagtatattt ctgttgaagt tacagaaaaa gtacttatgg aaacttttga
     4141 aattgtaaat gttactcttg ataaactaaa aaaagaaggc attagcatag ctttagatga
     4201 gtttggttct ggatttagta gtataaatta tctggcaaat ttacctattg aaaatttaaa
     4261 aatagataag gaattagtag aacaaattac ctattctaaa aaacaatttg aattattaaa
     4321 aactatagtt caaattacta agattaatgg catggatatt gtagcagaag gtgttgaaac
     4381 aaaagaaact ctggatttag ttgtaacttc aggtgtgaat ttgattcaag gtcattattt
     4441 ttcagaacct ttaagtgaag ataaatttat tgaacttatg attgaagaat aaaggttaat
     4501 aaaataaaga tttaaggtat tgaaaaataa ttaaaagtat ttaataatag aattagggaa
     4561 gaataagttt ataagtaaaa acaattgatt agtagttaat aaaataaatg tgatatccaa
     4621 ataaatccta aatctaattt gagttttaat atagatagtc taaagtttga ttattaaaga
     4681 tttaattgta agttgttaca gttgatgata ttgattagag attaaagttt agagtagaaa
     4741 gttaccttaa atttaaaaat gcaagataac ccaatctaaa aattataatc actcgatagg
     4801 tttaaggatt tatttgttta ttataaatag gagggtgtct caaaatgaac tttttagttt
     4861 atgaggcact ctcttttata tagaattaaa tatattttat tatttcaaca atgaaaaaga
     4921 agcttatctc tattttgaga cagcctcttt gtatactatt attaatttaa tcaataacat
     4981 aatttaaaat tataatacta gttaagattt cctgctttat tagtaaataa ttctttaata
     5041 gcattgtttc tggaaactaa atcgttatca gagttgtttg tttttttcat aatcactgaa
     5101 taacgttttt tatttataaa aaatattaaa atcttatcat catcaaaatt gtattcttta
     5161 tatctgtcga tagactcata acctagattt agaatgtttt tgtcttttat actatctaca
     5221 tctaaaatat atgatgggtc agcctttagt aatatattaa ttaaacattt ttcttcacca
     5281 gcacagaata actccatctt acttgatata gatttagatt gctctgaagt tatttttgca
     5341 ggaataaggt tgtcattatc atctcttaga agataaccat gtgtgtcttg ttgatatact
     5401 aaattaacct ttttcttagg aacactaatc attttatcta aatgtacata atgatgattt
     5461 tcaacaaaac cttcatttaa tatttcaaca aagttattaa ttttacctag gcaaaaagat
     5521 tctatagatt ctttacagaa tttatttgta aatggtatta tgtttacgtt ttcctcgtct
     5581 gtattccatg ctatagcata atccaacgaa tatacttctt cctctatatc tgcaaaatta
     5641 tattgtacta tgtttccttt caaaattact cgacctccat cttataatat atttaatata
     5701 ctttataaaa cttatattat atttaaataa tatcataaat tgaataaata tgatatagta
     5761 aataagaaaa aatttgaaaa aaatcactat aaatgagtaa taaatataaa tagtgtatac
     5821 taatgggatg gaaaatgtcc actattaact ttattacccc acatatataa gataaaatta
     5881 ttaatataaa atataatcta tcgacaatga atagactttt aaataaactt aatataaaat
     5941 taattaaata agtgcttaaa aacgatttac tttaatgata gaatcatatt aaaggaggat
     6001 aattttatgg atataaagaa taaaattaaa tctatatttt taccagaaaa aaatgactat
     6061 ttagatgatg aagaatttga gtatgatata gaaaataatg aagaagattt aaaggaagat
     6121 aacaaagtac atttaaatga tttaagcaga gtaaaatatg attacagaga gttaaaagac
     6181 atagaaaacc aaacagaaga gatgtgtctg ctagcagtaa agcaggatgg tactataatt
     6241 gagtttgtaa aggacaagac ttataaagta tgtatggaag cagtaaaaca gacatataaa
     6301 tctcttaagt atataacaaa tcaaaatgag gatatttgta tagaagcagt aaaacagaac
     6361 tacagagctt tgtactacat aaataataag acagaaaatg tactaattga ggctataaaa
     6421 aatgcatcaa cttatgatgt catggaagta tttaaatttg tggaagagca aacagaagat
     6481 gtatgtctag catttataga aagggcatct aaaaatgatg ttgcagagat attaaaagga
     6541 ataaaagagc aaactccagc catatgttta gaagctgtaa aaaaagatgg aaaatcttta
     6601 gcttatgtaa aggaacaaag taatagtata tgtttagaag ctgtaaaaga aaactacagt
     6661 gcactatctt gtgtaaaaga gcaaactgaa gaaatatgta tagaagcagt aaaacagaat
     6721 gactttgcat tgtattatgt aaatgaacag acagaaaaaa tatgtatgga agcagtaaaa
     6781 agaagttata tggctctaca gtatgtaaat aagcaaacag aagagatttg tttggaagca
     6841 gtaagaattg atggaagagc attacagtac gtaaaagagc aaacagaaga aatttgcttg
     6901 gagtcagtta gacaaaatgg aaaagtactg caatatgtaa aaaaacagac tgaaaatata
     6961 tgtatagaag cagtaagagg ttcatttgaa gaacttgaaa taaaagaaat attaagttat
     7021 gtaaagatac caacagaaag aatatttgta gaagcagtaa agcaaaatgg aaaaattctt
     7081 aaatatgtag aaaatcagac agaattaata tgtttagagg cagtaagaga aaactataat
     7141 gctttagctt atgtaaagga acaaacagaa aagatttgtt tagaagcagt taatcaaagt
     7201 tatgaagcgc ttaaatatgt gaaagaacaa acagaagaag tttgtctaaa agcagtaaaa
     7261 caagattata gaatgttgaa atatgtaaat aatcaaacag aaaaaatatg tttagaagca
     7321 gtaaagcaaa attatagagc tttagaattt gttgataatc aaacagaaaa agtttgtctt
     7381 gaagcagtaa aacagaatag aaaagcacta cagtatgtaa aacaaaaaca aagttaaaag
     7441 gctaaaatat atagaaaaca tatgcaaata aataaaattt gatattttgt aataaataaa
     7501 atgggtatct caaaaggaac tttttagttt gtgaggtact ctttttatat gaaatgaaat
     7561 atatttttag ccttttaaca atggggaaaa ggtttatttc tattttgaca taggcttttt
     7621 atgcatataa agcttgataa aattgtttta ttataattgt tttgttatat aaataataag
     7681 aagaaaatta tataggtctt tttttgacca gttttacatt tttaacaact agttttgtaa
     7741 agtatattga attttctcaa aaaagaatgt actctatata tgtaaaattt ttttggaggt
     7801 aaaaatgttt aaaagatatg ctgaaaaaat gacatctgtt ttaatttgta ataatatgat
     7861 agataataat gaatctaaag tctattctta tggatttgaa atattaatag ctttcatagt
     7921 taatataacc accatgctgt ttataggttt tttatttggt aaatttacat atgtattatt
     7981 ttttttaatg tgttattgtc caataagaca attttcagga gggtatcatg cagacaatta
     8041 ttttagatgc ttattaacct ttatttttat aattttatct acaatcttaa ttatagaaaa
     8101 tataaatata gacttattta aaaatattat tatgattatt gcaagtgtta gttgggttgg
     8161 aatttgtgtt ctgtgcccaa tagaacatag gagtaaccct ataagtgata gggaaaaatt
     8221 ggtttataag aaaactgcta tatttatatc tacagtagtg ttattgataa caattttaag
     8281 tctttctttg agtatatttg tagattattt cacatatagc gcatttgcca tgttttggat
     8341 ttttgtgatg ttagtattag gtaagttaaa agctaaagta taagtaaaat atgtatgagg
     8401 gagagttaag ttatgaaaaa gtttattgta agatttatga agtttgctag ctcattggca
     8461 cttagcactg ctattttgtc agctaatagt acctgtcctt ggattattca tcagcctaaa
     8521 gtacccaaag aaatcagcaa tctaaagaag acaaattaaa tacaagcact tatttataca
     8581 aaattatcag gaaaaaagaa attattatat tttaataaga ttaaaacaat ataaaatcag
     8641 atacaaaatt tattttattt attttatagg agcctaatac ctatttggta atatattagt
     8701 gcgccaattt agggggatat agtgtattta atgaaatgac ttacggagag taagtcattt
     8761 ttgtattgta tgtattttat gtaaacttat gatataatga agggtaaagt ttaattaaaa
     8821 taataaaaaa ctttagtata gatagtaatt taggaggtag ggtatgagta tgatacataa
     8881 attttctatg aatggatata atatcgtttt agatgtgaat ggtggagctg ttcatgtgtt
     8941 agatgatgtt gcatacgatt tgttagattt ttataaagaa aaaagtaaag aagaaatatt
     9001 agagatatta aaatcaaaat atcaagaaga aaaaataaat gaagcatatg aagaaatatt
     9061 aaatttggaa aaagagggtc ttttatatac agaagataca tatcaatatc atccaagctt
     9121 tgtacataga gagcctgtag taaaagcttt atgtttaaat gtagctcatg attgtaactt
     9181 aaaatgtaaa tactgttttg cagctcaagg tgattttgga ggagaaaaag aacttatgag
     9241 ttttgaagtt ggtaaggcag caattgacta tttgattgct aattcaggaa gtagaaagaa
     9301 cctagagata gatttctttg gtggagagcc tttgatgaac tttgaggttg taaaacaact
     9361 ggttgactat ggaagaagtg tagaaaaaga ttataataaa aatataagat ttacaataac
     9421 tacaaatggt gtactgctaa atgatgagat tatagattat ataaatgaaa atatgcataa
     9481 tgtagtttta agtttagatg gaagaaaaga agttaatgat aatatgagac caactttaaa
     9541 tgataaagga agttatgata ttacattacc acgatttaag aaacttgtgg aaaaaagagc
     9601 aaaagataag tattattata taagaggtac ttttacaaga gataatttag atttttcaaa
     9661 agatgttatg cattttgcag atttaggatt taaattaacc tctgtagagc cagttgttgg
     9721 agatgagagt aatccatatg cacttagaga agaagattta cctaagatat ttgaagagta
     9781 cgaaaaattt gctgtagagt atgcagatag acaattacaa ggagatggat ttaaattttt
     9841 ccattttatg atagacctaa atcaaggtcc atgtgtaata aagagaataa ctggatgtgg
     9901 agctggaaat gagtatcttt ctgtaactcc aaatggagat atatatccat gtcatcaatt
     9961 tgttggaaat gaagaattta aaatggcaaa tatatttgat gaagaaatag tacttccaga
    10021 aaatttaaaa aatatgttta gagaagctca tgtatatact aaagaagagt gtaaacaatg
    10081 ttggaataag ttttactgtt caggtggatg tcatgcaaat gcgataaact ttaataatga
    10141 catatcaaaa ccatatgaat taggatgtga aatgcaaaga aaacgtacag agtgctctat
    10201 aatgatacaa gctaagttaa tgttagaagg agcaaccaac taaatttata ttttaaagga
    10261 gttgtttata tatgattaga gattatttag aagataaacc tttaattgac gaaagtgtat
    10321 ttgtggcaaa atcagcagat gtaattggaa atgttaaaat aggcaaagat tcaagtatat
    10381 ggtacaatgc agtagttagg ggtgatgaag gaccaataac tattggagaa aacactaata
    10441 tacaagattg ttctatagtg catggagata cagaaactat aattggtaac aatgtaacag
    10501 ttgggcatag gtctatagtt catggttgta aaattagtga taatgtgtta attggtatgg
    10561 gttcaataat attggataat gctgaaatag gagaatatac tctgatagga gcaggtacac
    10621 taattacatc taataagaag tttccaccag gtgttttgat aatgggttct cctggtaagg
    10681 ttgttagaga gcttacagaa gaagataaaa aatatattga tgaatcttat gaatggtatt
    10741 tagaggcagc acaaaatcaa aaatattagt ttaaagagtg gttgtttcaa aacatattta
    10801 aatctgtttt gaggcaacca ctaatttatt ttattagatt ttgttgatat tttagttttc
    10861 aaaaagtatt tgattaaacc agatataaaa tgtcaaaaat aaaaattaac tgagttaaat
    10921 aattttaata aaattggtag tatatgtgat aaaattaaat attaaagagg atataggata
    10981 ttagaatatc aattggtgaa ataatgtcat atacgataaa ttaaggtgta agtttttggg
    11041 tatacctatc ttaggagtga aaagatttga gaataagtta tagaaattta aagcgatata
    11101 gagaaattgg atatgtttta ataaagtatg gttttagttt tattgtagaa agactaaata
    11161 tagaaggaat tgcctataaa ataccactat ttgacccacc agaagaaata aaaaatatga
    11221 caactggtga gagaatgaaa agagttttag aagaactagg acctacatat ataaaaatag
    11281 gacagatatt gagtacaaga aaagacttgt tagaccagga tataattgat gaaatatcaa
    11341 aactcagaga tgatgtagag aaatttgatt caaatatagc tattgatata tttaaagaag
    11401 aagtgggttt aagtatagaa gaaatattct tagaatttaa agaagaacca atagcagcag
    11461 cttcaattgg tcaagtttat gaaggtgtac ttaaaacggg agaagaagta atcgtaaaaa
    11521 ttcaaagacc aaatatagag aaaataataa aatctgattt agagattctt agaactattg
    11581 caaatacttt aaaagattta aaaaaggatt ttaatttaga tttagtacag atgattgaag
    11641 aatttcaaac tcaattaatg agggaacttg attatacatt tgaagctata aatgcaacta
    11701 aattctctag gatatttaaa aatagtgatg aagtatatat acctaaagta tattcagaat
    11761 ataatactaa gaaaatttta gtaatggaga aagttaatgg tacaaagtta agtgatgtag
    11821 aaaaaataag gagactaggt tataacacaa aaactattgt tgaaatagga gttaggtcat
    11881 tttttacaca ggtattatct catggatttt ttcatgcaga cccacatcca ggtaatatat
    11941 tcgtagttgc taaaaataag atagcatata tagattttgg aatgattgga attattgata
    12001 ataaaacttt aaaccaatta aatgaaattg cattagcagg tgtagaaaag aatgttgaca
    12061 agataatata tctattgata gaaatggatg cattaaatgg agaggctgat attaagggac
    12121 ttagacagga tttactttat ctaatacatt attactatga tatttctata gaaaaaataa
    12181 atgtaacaga tatattaaat gaactattta ggttttttag acagtataaa atagtcatgc
    12241 ctgcacaatt tgtaactctt gcaaagacag ttataacatt agaaggaaca agtagaactt
    12301 taaacacaga tttttctttt ggttctatgg gaaaagagtt tatgaaacat cattataaga
    12361 gtaaatttaa ccctaaaaat gttgttttaa gttcaagaca aaatgtagaa gaaatattac
    12421 tggacataaa aactattcca aagcaattaa aagcaatttt aaaaaatata gaaagaaata
    12481 atataaaaat gcaaattgaa gatgttaaaa tgacgaggtt agaaaattgt ataatagagc
    12541 taacatctca aatatctttg agtttagtgt tggcatctat tatagtaggt tcttctctta
    12601 taattgcatc tccaaatata gaaaataata tatggataaa atttactgca atagcgggtt
    12661 tctttatatc ctttataata ggcctttgct tagttataag aagtattagg tcaaaataca
    12721 aaaaagatta gggggaatta aatttgaaaa aatggatatt gaagtataaa ggtaagatag
    12781 aggaaagtga atttagcaaa aaacttaata tttccccgga aatctgccaa attttaaaaa
    12841 atagagggat atgtactgaa aaagattcag aaatatttat gaatccatct ttagattatt
    12901 taagagaccc ttttttaatg aaagatatgc aaaaagctgt tgatagaata aagttagcag
    12961 ttgagaagaa cgaaaaaatt tggatttacg gagattatga tgttgatggt gtatcatcta
    13021 catctatctt gtgcttatat tttaagagta taggttacaa tgttgattac tatattccta
    13081 atagattaga agaagggtat ggtataaatg aagatgctat taagcttata agctctaggg
    13141 gatgtgactt gattataagt gttgactgtg gaattacatc agtgtctgag gtcaatacag
    13201 cgaatgattt gggcatagat gttataatta cagaccatca tgaatgtcaa agtgaaattc
    13261 catcagcctt tgcagtaata aatccaaaac aagaagattg taattatcca tttgattcac
    13321 tatgtggatg tggtgtagca tttaaaatga ttcaagcctt gactccaaaa gaagaattta
    13381 aaactagtat gtataattat ttggaaatag taacactagc aactatttgt gatattgttc
    13441 cacttataga tgaaaataga ataattgtaa aaaatggttt aaaatcaatg aaagaaggca
    13501 aaaatattgg attaagggag cttataaaag tttgtggtgt agaaagtgat aagataggtt
    13561 cttctcatat aggttttgca ataggtccta ggataaatgc ttcaggtcga ttgggatatt
    13621 cttacttagg agtagagcta tttactactc aatcacaaga agaggcagta gaaatagcta
    13681 gtatattgga agaaaaaaat aatgaaagac agatgataga agctaagatg tatcatgaag
    13741 ctgaagaaat gctaaagtct aatagcagat acaatgatga caaggtgttg gttttagcta
    13801 aagagggttg gcaacatgga ataataggaa ttgtagcatc aaagttaact gaaaaatact
    13861 acaaaccaac aattcttcta ggtatagaaa atggggaggc tactggttca gcgaggtcaa
    13921 taaaaggatt taatatattt gaagctctca taaagtgcaa agacttgatg actaagtttg
    13981 gtggtcatga acaagcagca gggttatcct tggattcaga caatgtagaa attttagcaa
    14041 atgaaataaa taaatttgca gattataatt taactgaaga tgatatgata gagaatgtaa
    14101 atgttgagtt tgaattacaa gaaaatgtaa taaatttaaa tcttgtagaa gaattacata
    14161 agttagaacc ctttggatta aataatccta atcctagatt tatagttaga aattatattt
    14221 taaaagattt gaaagttata ggtaagaatc aacaacatct gaaattaagc atagaaaaag
    14281 agaaaagcta tgaatgtata ggatttaata tgagtcattt aaaatcaatg tataaagtag
    14341 gagacaaggt agatgtacta ttccaattag atgaaaataa ctatatggga aataggaaag
    14401 tacaattttt acttaaagat atacgattgg ctcgacctaa aagtgcaagt aatgacaaac
    14461 tttcattgaa attaatgtca aaaataatac caaaagatac tcaaagcctg tataatatat
    14521 ctgtgtctga ttttgaatta tttgatggaa atacagatat aaatatattt gattattttg
    14581 aaaaagatac actaataata tctaattcta taaatggatt ttatagagca atgtcagata
    14641 tatcacttat agatttggat tttaacataa attacaatat tattgaagat gatagcaaaa
    14701 atactgataa attagagctt atattttctc ctaatattga taaaatagac ttaaaaagat
    14761 ataataatat tattctttat gattatttat acaataaagg cgaatattcg tatatatatg
    14821 agaataaaag agaagaaagt gaaattataa aatattataa taaaacagac ctattatatt
    14881 taaagaatgt agttagtaat atagttccat caagagatga atttataaca atttataaac
    14941 aagcactcat aaaaaaagaa attgatttgg atatggtaaa tataagggaa acttttaatg
    15001 taattccatt aaagtttttt acgatattaa atgtatttag agaattaaat ttacttgact
    15061 ttaatttaaa ttatgaaaaa aattctgtac tcataagaat attgccaaaa ccacagaaaa
    15121 aattagactt aaatgagagt ttaatactca ataatctaaa aaatttagaa aaacaataca
    15181 atagcagtta ttaggaggcg tttcccatgg atttaaaaaa ctttataagg aatattgatg
    15241 atttcccaaa gccaggcata gattttaaag atgtgacaac tctatttaaa gatggagatg
    15301 catttaaata tgcagtggat tctatagttg aagaattgaa agataaagat gtagatttag
    15361 taataggacc agaagcaaga ggatttttga tgggtacacc tgtagcatat gctttaggag
    15421 taggatttgt tccaattaga aaacctggaa agttaccagg tgaagttgag agttatgagt
    15481 atggattaga gtatggtaca gatactttag agatacataa ggatgcaatt aaaaagggtc
    15541 aaaaagtagc aatagtagat gatttattgg ctactggtgg aactatggaa gccgctgcca
    15601 agcttgtaga aaaattaggt ggagaagtag tttctatgca atttttgatt gagctaaaat
    15661 tcttaaatgg gagagaaaag ttgtctaact atgatgttaa ttctcttata aaatactagt
    15721 acttttataa tagttggtat aacaccaact attttcatat atattaaaat tttatacaaa
    15781 ataggtggtt atatgcatga taaagaatta caagaaataa ttgaaaaaat aaaagtttat
    15841 gcacctaatg caaattttga aatggttgaa aaagcctata atcttgcaaa aaaagcgcat
    15901 gaaggacaat atagaaaatc tggggaacca tactttattc atccattagc agttgcaaat
    15961 attttaatag agatggaatt agatattgaa actataacag caggtttact tcatgatgtt
    16021 gtagaagata cagaatatac ctatgaagac ataaaaaaag aatttggcaa agaagtagcg
    16081 gatttggttg atggagtaac taaacttggt caaataaaat atcgttcgaa agaagaaact
    16141 caatctgaaa atttaagaaa aatgtttttg gcaatggcga aggatataag ggttatactt
    16201 ataaaacttg ctgatagatt gcacaacatg agaacattaa aatacatgcc tccagaaaaa
    16261 gcaaagtata aagcaaaaga gacacttgaa atttatggtg gtatagctca tagattagga
    16321 atttccaaga taaagtggga actagaagac agagcattaa gatttatgga cccagaaggg
    16381 tattatgatt tagttagtag agtatcaatg aagagaagtc aaagagagga ttatattcaa
    16441 ggaattgttg aacttcttaa agataagttt aaagatttaa gtataccatg tgatgtatat
    16501 ggtagaccaa agcattttta cagtatatat agaaagatgc aaaaaaaaca taagactttt
    16561 gaagagattt ttgatttaac agcagtaagg atattggttg ataatgtaaa ggattgctat
    16621 gcagtacttg gtatagttca tacattatgg agacctattc caggaagatt taaagattat
    16681 atagcaatgc caaaacccaa tatgtatcaa tcattacaca ccactgttgt aggtcctgat
    16741 ggagaacctt tagagataca aataagaact catgagatgc ataatatagc tgaaaatggt
    16801 atagcagccc attggaaata taaagaaggt atctctagca aagagtcaaa ggttgaagaa
    16861 aaacttcaat ggttgagaca gatgatggag tgggaaaaag atttaaaaga cccacaagag
    16921 tttatggatg cacttaaaga agatgtattt aatagtcaag tttatgtatt tacgccaaaa
    16981 ggagacgtaa ttgaattacc agcagaatca acacctatag attttgccta tagagttcat
    17041 acaaatgtag gtaataaatg tgtaggagct aaaattgatg gaagaatagt tcctattgat
    17101 tataagcttc aaaatggtaa tattgtcgag gtacttacat catctaattc taatggacca
    17161 agtagggatt ggcttaatat agtgaagacc ccaaatgcaa aaagtagaat tagacaattt
    17221 ttcaagaagg aaagaagaga agaaaatgtt gaccgtggaa atacaatatt agaaagagaa
    17281 tttaagaagc atgatatacc acaaaaagac cctcttgttg aaaaatatat ggcattagtt
    17341 gctaagaaat ttaatcagcc aagtgttgag gacttaattg ccactgtagg ttatggtggt
    17401 ataatgtcat ctcaagtggt atctaaggta aaagacctat atttaaaaga ggttaaaaaa
    17461 gctaaaaagg aacaacagat acaagaagaa gatttaaata aacacaatat aagtgaaaca
    17521 gaatacagta aaaagagaaa gaaaaacgca tcacaaggaa ttatagtaaa agggcttgac
    17581 aatatcttag taagatttgc taagtgttgt aatcctcttc caggagatga aattgtagga
    17641 tatattacta agggtagggg agtagccata cacagagctg attgcccaaa tgcaaacaat
    17701 aatggtgatt tcttccaaaa tagattggtg gaggtttcat ggagttcagc aggaaaaggt
    17761 aagtttgaag cggaaattca aataaaagct attgacagaa gaggaattgt taatgaaata
    17821 actcatattg ttgcaatgga taaacttagt ttaaacggaa taaatgcaag aaaaggtaag
    17881 gatagtattg taagtataaa cttacttgta gaggttaatg acacagagga attaaaaatt
    17941 ctaatgaaaa aattaaaagc aattcctggt gtagaaagta tttatagaat gacaaattag
    18001 agttaaaata atagggagga aactatgaga gcagtagtgc aaagagtatc ttcatctaaa
    18061 gttacagtag atgaaaacac tataggtcaa ataaataagg ggttactagt attgttagga
    18121 gtgactcatg atgataaatc aagtgatgta gattatatga ttgataaaat attgaatctg
    18181 agaatttttg aagatgaaaa tgacaagatg aatttatctc taatggatat aggtggagag
    18241 cttttagtag tatctcaatt tactttatat ggagattgca gaaaaggaag aagacctgga
    18301 tttagtaatg cagctagacc agaacttgca aataaccttt atgaagaatt tgtaaaaaaa
    18361 gcaaaagata aaggtgtgac agttggtaca ggtcaatttg cagctcatat gatggtagag
    18421 ttaactaatg atggtccagt aacaatacta ttagactcaa gtaaatcttt ttaaggggga
    18481 aaactttaat gattataaaa aaatttgtag atagttattt tggagtaaat acttatgtat
    18541 taggtgatga aaagactaaa aaatgtgcag ttatagaccc aggtggaagt ttagtagaga
    18601 cattaagttt tgtaaaagaa aatgaattaa atattgaata tattatcctt actcatgggc
    18661 atggagacca tataggatat gttaaggata ttaaagaaaa aactggtgct aaagtagttg
    18721 cacatgttga tgaaaaagaa ttattaaatg acaaaaataa aaatctaagt tatacaatga
    18781 gatgtggagc gcaagagttt gatgcagata tctatgtaaa tgataaagac aagcttgaac
    18841 ttggtgaatt aaaaatgact ttcttgcata ctccaggaca tactcaagga tgtatgtgta
    18901 taagagttga agatgaaatg ttcacaggag atactttgtt tgctggaagt attggtagaa
    18961 ctgatttata tagtggagac tttaaccaaa tggaaaaatc acttaagaaa ttatgtaaat
    19021 atgaagatgc tgttagagta tatcctggac atggaccaac cagtactcta ggggttgaaa
    19081 agaagacaaa tccttacatg taattttata ttagtattta aaaaagtata aaatatttaa
    19141 taactatatt agtgaggggg ttcttctact gttaaataga aggagttaag ttgatgttgt
    19201 atgttttttt aaaaggacat gattataagt atgaagtagc agaacttata aaattgttta
    19261 ctagtgaatt taagtttaaa gactctaatg ctaacaataa tatagagtca aagcatttaa
    19321 taaatagatt gatttatgag aatggtgtat tattctcaag tactgaatac tatgaaaatg
    19381 gaaacttaaa gtacgagtca atacagaata tagaaaatat gaatcttgaa tttttagatg
    19441 gatttgatag cagcaaaacg attgaagaat ttcttaatag cctagatagt gaaagtaaga
    19501 ggaactttaa aaagctgtgt aaagaaaata tcaaaaaaag catgttttta gtgcttaaac
    19561 aagtatttaa ttcttatgta ccatggggaa ttttgactgg tattagacca gtcaaaattg
    19621 tccacaattt aatggataaa aagctagatg ataatagcat aagaaccatt ctaaaagata
    19681 attattttat aatagatgag aaaatagatt tagctcttga aatagctaaa agagagcgat
    19741 tatttatgta tcctatagat aaaaataaaa tttctcttta tgtaagtatt ccattttgtc
    19801 caactagatg tgtatattgt tcatttcctt caaattcatt aaaacaattt ggacatttaa
    19861 aaagagaata tgtttataaa ttgattgaag aaattaaggg gtttgccaaa gttattaagg
    19921 atactaagaa agaagttgaa actctatata ttggtggtgg aactccaact acacttgatg
    19981 aggaagaatt ggatttgctt ataaattcat tgttcaatga attagattta agtaaaatta
    20041 aagaatttac tgtagaagca ggtagacctg acactattac agaacaaaaa ctaagagtgt
    20101 taaaaaaaca caatgttagt agaattagta taaatcctca aactatgaat gatgatacct
    20161 tggtaaagat tggaagagag catagagtat ctgatttagt agattgcttt aatatggctc
    20221 gtagaatggg atttgataat ataaatatgg atataatttt aggtcttata gatgaagact
    20281 taaatatggt aaagaacaca ttggaggaga taaaaaaact ttctcctgaa agccttacag
    20341 ttcatacatt agccataaag agagcatcta acttaaatat aaatatggat aggtataaag
    20401 aacatctaac acaatatgaa gaaatggtaa aaatgataga tttatcaatg aaatatgcaa
    20461 aagatatggg attaaatcca tattatatgt acagacaaaa acatatgttg ggaaatttag
    20521 aaaatatagg ttatgctaaa gaaggatatg agtgcattta taatatacag attatggaag
    20581 aaaaacaaag taactatgca ttaggtgcag gtgctatatc gaaatttgtc tatgtagatg
    20641 aagatagaat tgaaagagta gaaaatgtaa aaaatgttga acaatacatt gaaagagtag
    20701 atgaaatgat aaaaaggaag aaagaagagg tatataaaaa tgttgactaa agctcctaga
    20761 ggaactaaag acataactcc aaaagaggct tacaaatggc gttatgtaga aaataaattt
    20821 agagaaatat gtgctttata tggatatgaa gaaatggtaa cacctatatt tgaacataca
    20881 gaacttttca agagaagtgt tggagatact acagatatag ttcaaaaaga aatgtattct
    20941 tttaaagata aaggtgatag agaaattacc ctaaagccag aaggtacagc aggtgtagta
    21001 agagcattta tagaaaataa attatatgca gatactcaac caacaaaatt attttatgtg
    21061 acaccttgtt ttagatatga aagaccacaa gctggtagac aaagacaatt tcaccaattt
    21121 ggaatagaag cacttggaag tgatactcct tcaatggatg ctgaaataat cgcactagct
    21181 gttcaatttt ttaatgaagt tggattaaat gatttggtag tgagtataaa ttcagttggc
    21241 tgtccagttt gtagaaaaga gtacaatgct ttattaaaag agtatcttga ctcaaaagct
    21301 gatatattgt gtgatacttg caatgagaga agagaaaaaa atccaatgag ggttatagac
    21361 tgtaaaaatc ctacatgtaa agaaaatatc aaagatatac catttattgc agaccatttg
    21421 tgtgatgatt gtaaatctca ctttgataaa cttcaagaat acttaaagga aatgaatata
    21481 aactttgtaa ttgataaaac aatagttaga ggcttagatt actatagaaa aactgctttt
    21541 gaaattatat ctaatgatat aggagctcaa agtactgtat gtggtggagg aagatatgat
    21601 ggtcttgttg aacaattagg aggaccaaaa ggtataagtg gaattgggtt tggactagga
    21661 attgaaagac ttttacttac acttgaaggt aatggaattg aaatagaaaa ccctcaatct
    21721 acggatatat ttatagtaac tataggtgaa gaagcaaata caagaagctt taaattgtta
    21781 aaagacttga gacaaaatca tataagtgca gataaagacc atatagagag aagtgttaaa
    21841 gcacaattta aatattcaga taaaataaat tcaaaattta caatagtaat tggagatgat
    21901 gagcttaaaa atgacactgc gacattaaaa aatatgaaaa catcagagca aactactgta
    21961 aaattaagta cattagttga agaattaaag caaaagctgt aaaataagaa agtatgatgt
    22021 tttgtgtatt agataaatat atagcattaa aattaaataa aatggaggta gtatcttgga
    22081 aactttaaag ggtttaaaga gaactcacta ctgtggagag ttgagagaaa aaaacataaa
    22141 tgaagaagtc gtacttatgg gatgggtaca gaaaaaaaga aatttaggtg gtcttgtatt
    22201 tgttgattta agagatacaa gtggattatg tcaaatagtt tttgatacag atgtagataa
    22261 agaagctttt gaaaaagctg aaaaattagg ggctgaattt gtaatagctg taaaaggaaa
    22321 agtatgcgaa agacaatcta aaaaccctaa tatgctaact ggtgatatag aaatatttgc
    22381 aactgaactt agacttttaa acaagtcaga aactccgcct atttatataa aagatgatga
    22441 tgatgtttct gaagcactaa gattaaaata tagatactta gatttaagaa aaccatctat
    22501 gcaaagaaat ttaaaattaa gacataaagt aatgaatatt acaagaaatt acttatcaaa
    22561 caatagattt tgcgaaatag aaacaccatt tttaatagct ccaacaccag aaggagcaag
    22621 agattatcta gtaccaagta gagttaatcc aggaaaattt tacgcattac cacaatcacc
    22681 acaattatat aaacaattat tgatggtaag tggtatggat agatatttcc aaatagttaa
    22741 atgctttaga gatgaagact taagagcaga tagacaacca gagtttacac aaatagactg
    22801 cgaaatgtct tttgttgagc aagaagatgt tatgagtatg attgaaggtt tgcttgaagc
    22861 gatatttaaa gaagttttag atgtagaatt ggcacttcca cttcctaaga tgacttatgc
    22921 agaagctatg tctaaatatg gttcagataa accagatact agatttggat atgagttaac
    22981 agatatatct gatgttgttt gtaactgtgg atttaaagtg tttgctgatg ctacacaacc
    23041 aggaaaaagt gttagaggta taaacgtaaa aggcaaagct gacgacttta ctagaaaaca
    23101 aatatcatca ctagaagaac atgctaagac atatagagca aaaggtcttg cttggatgaa
    23161 agtaggacaa gaaggagtaa cttctccaat agcaaaattc tttaatgaag aagaaatgaa
    23221 cgctatactt acaagaatga atgctgaagt aggagattta cttttatttg tagctgataa
    23281 aaactcaata gtatttgatg ctttaggaca agttagactt gaagtagcaa ataggcttaa
    23341 tctattggat aaaaatgtat ataacttact atgggtaact gagttcccag tttttgaaga
    23401 agatgaagaa acaggcacat tctcagcaat gcatcaccca tttacatcac ctatggatga
    23461 agatttagac aagttagaag aaggtgataa gtcatcttta agagcgaaag cttatgatat
    23521 tgtattgaat ggttatgaaa taggtggagg aagtgttaga atatcaaatt ctgatgttca
    23581 atcaagaatg tttaaagcac ttggttttac agaggaaaga gctaatgaaa agtttggtta
    23641 tttattagaa gcatttaaat atggtactcc tcctcatgct ggtctagctt ttggtcttga
    23701 tagacttgtt atgttacttg caggaactga taatataaga gaagttattg cattccctaa
    23761 aaatcaaaat gctgtttgcc ctatgacaaa tgctcctaca ttggcggaag atgagcaatt
    23821 agaagaatta agtataaaag ttgatataaa agataatgaa taatatgtag ttgcaagaaa
    23881 ctaaaatgta aaaagtctgg atataaaata tatttatatt cagacttttt ttattagaat
    23941 tattttatat tttttctcta tatattttca taaattatac ttatattctt aaccaatata
    24001 attaaaatac tatgtttaaa aattttacat aaacttgatt attttagaaa ctacttctg
//
LOCUS       DAMJVI010000055        23655 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.057, whole genome shotgun sequence.
ACCESSION   DAMJVI010000055 DAMJVI010000000
VERSION     DAMJVI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.057"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            270..1121
                     /locus_tag="PC545_002229"
     CDS             270..1121
                     /locus_tag="PC545_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HDF2456996.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVVGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKKTKEI
                     KSTEGEC"
     gene            1125..1595
                     /locus_tag="PC545_002230"
     CDS             1125..1595
                     /locus_tag="PC545_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2456997.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            1608..2918
                     /locus_tag="PC545_002231"
     CDS             1608..2918
                     /locus_tag="PC545_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2456998.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYKNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKKGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHASDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVEITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIIPAK
                     AMALTVVDLLFDNAKDAKDILDTFTPVMTKEEYLTFLEKHDKTL"
     gene            3130..3876
                     /locus_tag="PC545_002232"
     CDS             3130..3876
                     /locus_tag="PC545_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2456999.1"
                     /translation="MFNAGNRIINTWLYPIQDGFVLIDTGYENGFKHFKKRISKFNIK
                     IKDIRYIFLTHAHDDHVGFLNQILDESPNTKIVMHDKALEILYKGQNSFIGGCTSRTA
                     FLFCQIMKLFGKGEHSFSPLKQEFEKRCILVSDKNRKEIEKELNGKIIDTPGHTADSI
                     SLLIDEGVLFCGDSAMNGFPSTHKITIFAESKLEFIQSWKTIINTKPKMIYPGHGKPF
                     EYSKLEQNLIYVNKINLYPLHNSKDIHRFL"
     gene            4238..5779
                     /locus_tag="PC545_002233"
     CDS             4238..5779
                     /locus_tag="PC545_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2457000.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SLVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAELDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMVFNIPLGPGIL
                     PSYSM"
     gene            5814..6920
                     /locus_tag="PC545_002234"
     CDS             5814..6920
                     /locus_tag="PC545_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2457001.1"
                     /translation="MLDKNRLINNFMDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPILDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPDNLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDKVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHSMSYPSFELSRDSHVFKLSEEAIRQVGVVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(7251..8537)
                     /locus_tag="PC545_002235"
     CDS             complement(7251..8537)
                     /locus_tag="PC545_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2457002.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDSDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFNLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRHSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAINENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(8660..11758)
                     /locus_tag="PC545_002236"
     CDS             complement(8660..11758)
                     /locus_tag="PC545_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2457003.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKAMGLNDISHLK
                     CYKALQGKDSPCEFCTNSILKEDEYYIWENTNTRTNRHFILKDKLIKWEGKHARLEIA
                     LDITEKEDISKSIAEKLEIKELLVGCIKHLITESNFSDAVNLVLTNIGKFHKADRAYV
                     FEVSEDRTKVTNTYEWCNEGVDEQKQALTNLDINELKNWFLLFEQKGFVIIDDIENIK
                     EEQPIEYEILKPQKIESLMTSSLKQDDKVVGFIGVDNPHKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTGAYNRNKYIKYIDNFQKGKVKSIGVVFVDINGLKDIND
                     NFGHANGDSAIVSACVIIKKYFHSYNVYRIGGDEFVVLCEDISKQLFEELVSWLSEEF
                     KDEMIYSVAVGSIWSDDNIDIHKLIKVADERMYQDKKRYYNKNKLRNNEMKVISDLAD
                     STNNILFKHKELVDKVYFDDLMIKGYSKAYEKLAYNTYDIILSICIDTNLITPIYQSE
                     SNIFSFPIKENLEKTFSYIKKYLIHPDDIILFNDNHLLKSLDSLNNSKQDDLELNFEY
                     RRLGIDKKYYWALIYMLKISSKNKLGEKQTHILVAIKNIDYLMQLHKNQKDTLTGLYN
                     YESFCEQISLMLKRYPDKRYAIIMMDIDKFKVINDIYGVRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDRDIHKVIYKIKNSLNRKKFEYMLVPCFGVYRILDNH
                     IPITNACEMAGYAHKKVKKQSINNYIFYDDTIRNDALEEKQMEREMEYALENGQFHVY
                     LQPKYNLNANKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIRLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYSIPKSFLELELTESLFIKN
                     IKLFTKIIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNEEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSSDFEKLLY
                     QK"
     gene            12483..12635
                     /locus_tag="PC545_002237"
     CDS             12483..12635
                     /locus_tag="PC545_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457004.1"
                     /translation="MKIFSFLLFVVGICFCIAGFMGHSSAFSGATLSFFATTMIAVRQ
                     VKTKKK"
     gene            complement(12928..13632)
                     /locus_tag="PC545_002238"
     CDS             complement(12928..13632)
                     /locus_tag="PC545_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457005.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQDNINNDAPKSFEVKNLKIDYENHIVSINGEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(13623..15230)
                     /locus_tag="PC545_002239"
     CDS             complement(13623..15230)
                     /locus_tag="PC545_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HDF2457006.1"
                     /translation="MKKERLQHEDLKEYDYDEDGYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGVACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESN
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPMYDEEGKDKWK"
     gene            complement(15484..16662)
                     /locus_tag="PC545_002240"
     CDS             complement(15484..16662)
                     /locus_tag="PC545_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457007.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(16888..17382)
                     /locus_tag="PC545_002241"
     CDS             complement(16888..17382)
                     /locus_tag="PC545_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2457008.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTKIKENEKYKKSINKI
                     KQQK"
     gene            complement(17383..18144)
                     /locus_tag="PC545_002242"
     CDS             complement(17383..18144)
                     /locus_tag="PC545_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2457009.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFVGVSIAVGMFLVLGLYFKLGGI"
     gene            18866..21700
                     /locus_tag="PC545_002243"
     CDS             18866..21700
                     /locus_tag="PC545_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457010.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKTGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKISGKPILFLHASIIENLIGRTLNSDEFSINNCNELLKSSKN
                     DSEDIFSTLLGHDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIKVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSELDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     SMYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     TIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEINYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNTKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     FTIIELIDENLKSKFKLGYILHCSFMIERCFKNTTFPYNNITELISKNHELYSLIREA
                     FKPTEAYFNILIPDEEIAYIIDMINEYLNK"
     gene            21743..22987
                     /locus_tag="PC545_002244"
     CDS             21743..22987
                     /locus_tag="PC545_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2457011.1"
                     /translation="MEDEILKGKIKQLTILALIFIFITPVFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAVDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAAIAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENMSTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMVPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSATLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKITVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVVKYYNDSGKFVGSVDVKSESNIGCIPLKDK
                     IKIKVAKINKKLEIKNSVCFKA"
BASE COUNT         8317 a         3674 c         3106 g         8558 t
ORIGIN      
        1 agctttttag ctattattcc tacaatattt aaacaatttt ttaataataa agctatatta
       61 caatatattc aaaatatgta atattttaga tatttttcgt tgacaatgtt aaatttttgt
      121 tttatcctta attaaggaca tgttattgac atttctgaaa cttatttgaa atagtttttt
      181 cattttattt aggaaaacgt attataccta agaaaacgta ttttctcatt tcagataagt
      241 aaaatattta ttcttttggg gaggaaatca tggaaattaa taaaaaacat tttattcaag
      301 tatttttaac ttgcttaatc ttggtagtaa tatgtgaatt tatagggcaa agaactatag
      361 ctattggaaa agcctctata gtactgttcc ctatgcttta tgcagttata attggactta
      421 taataacacc agatttatta ggtaaaaaaa ttaaagcact taaaaaagtg gttggagaaa
      481 aagaaataaa tattgctggt gaagttgttg gaattgcact agtaatgctt ggtataaaat
      541 atggtactac agttggacca aacattgaca aaataattca agctggacca gcatttatcg
      601 ctcaagaatt tggtcatata cttgcaccaa tcgttgcatt acctcttgca ttaatgtttg
      661 gaatgaaaag agaagctgtt ggagctactg caagtataag tcgtgaacct tctttaggag
      721 ttatatcaga aaaatatggg ataaactcac cagaaggtag tggtgtactt ggtacatatt
      781 taatgggaac agttcttggt actatatact ttagtgtact aggttcaata tctatttata
      841 gtggtcttca cccatatgct ttgggaatgg cctgtggtgt tggtagtgga agtatgatga
      901 cagcagctgc tggttcatta tctaccatgg taccacctga aatggcagat acaatacttg
      961 cctatgctgc aacaagcaat atgatgtcta gtataacagg aattatattc ttagtttttg
     1021 tatccttacc atttactaat tttatgtata agattttaga acctaaattt tcgagaaata
     1081 aaaaaactaa agaaattaaa tctacagaag gagagtgcta ataaatgttt gctaatttaa
     1141 atttaaagaa aagttcatat caattattgg ttatgtctgt tattggagtt ataattctta
     1201 taggacaaag aattagcgtt ggtacatcta tagttacagc tcttcctggt atggtgatgc
     1261 taatattagc tgctatggca gctatgatta taaaagattt atttcctaaa tcaatctttc
     1321 cagcttttgg atttgcaact ataatcggcc ttttacttag tatgccatat agtccaacat
     1381 ctgaagtatt cttaactaac actaataata ttaactttat ggctataact actccactac
     1441 tagcttttgc aggaatttct gttggaaaca aaatagaagc tttaaaagaa atgtcatgga
     1501 aaatagttat aatttcactt attgtattta caactatatt ctttgcatgt gcgagtatag
     1561 ctcatatagt tttaagtatt caaggtaaaa tataggagga atttattatg aataaagaac
     1621 aattaaaatc tctagtaata aaaactatag atgaaaatag agaaaaaata ttaaaaatag
     1681 gtcaaggaat atacaaaaat ccagaatatg gatataaaga atttaaaact acagaagctg
     1741 tttcaaactt ttttgagcaa gaattaaatt tatctgtaga aaaaaatata gcagttacag
     1801 gatgcaaagc agatgcaaat tctagcaaga aaggtcctca tatatctata cttggagaat
     1861 tggatggtat atcatgtaaa gaacataaag atgctaatga aataggtgct tctcatactt
     1921 gtggacataa tattcaaata gctggaatgt taggtgctgc tgttggttta gtaaaaagtg
     1981 gcgttttaga acatttagat ggtaaagtat cttttatggc tactcctgct gaagagttta
     2041 ttgagttgga atacagagag caattaaaaa aagaaggaaa aataacttat tttggtggaa
     2101 aacaagaatt aataaaaaga ggttattttg atgatataga catatctatg atgtttcatg
     2161 cttctgattt aggagaagat aaggctctag ttggtcctga gagtaatgga tttgttggaa
     2221 aaaaggttaa atttataggt aaagaatctc atgctggttc tgctccacat gatggaataa
     2281 atgctttaaa tgcagcaatg cttgctataa acaacgtgaa tgcacttaga gaaactttta
     2341 aagagtctga aagagttaga ttccacccta tcatcacaaa aggtggagat atagtaaatg
     2401 ttgttcctgc tgatgttcat atggaatcat atgttagagc tagaactata aatggtatga
     2461 ttgatgctaa tgaaagaata aataaggctt tgatggctgg tggaatggca gttggagctg
     2521 atgttgagat aactgaaatt cctggatatc ttccaatatt acgatacaaa agtcttgata
     2581 atttatttaa aaacaatctt gaagatttag gtctaaaagg aaaagttatt gatggtggag
     2641 attttacagg ctcttttgat tttggagatg tgtctcatat aatgcctact cttcacccta
     2701 tgattggtgg tgtaaaagga gctcttcata caagagattt tgaaatagtt gatgaagatt
     2761 tagcttacat aattcctgct aaagcaatgg cacttacagt tgtagacctt ctttttgata
     2821 atgctaagga tgctaaagat atattagata cctttactcc agttatgact aaagaagaat
     2881 atctaacatt cttagaaaaa cacgataaaa ctttataagt ctatttttat aatagggtgg
     2941 tatctactgt aaaagttata ccactctttg tttttgtaac agtatacttc ttttctttta
     3001 gtttttagat taaactaaga tatattatcc tccttaaaac ttttagagct acgttgaaat
     3061 atcagtagaa ctatgctata ttctttctaa ttaatataaa tttctattct aattcaaggt
     3121 ggtgaaaaaa tgtttaatgc aggaaatcga attataaata catggttgta ccctattcaa
     3181 gatggctttg tcttgattga tactggatat gaaaatggtt ttaaacattt caaaaaacga
     3241 atttctaaat tcaacataaa aatcaaagat attcgataca tatttctaac tcatgctcat
     3301 gatgaccatg ttggttttct aaatcagatt ctagacgaat ctcctaatac aaagattgtt
     3361 atgcatgata aagctttaga aattctatat aaaggtcaaa actcttttat tggtggttgt
     3421 accagccgta ctgcctttct cttttgccaa attatgaaac tatttggaaa aggtgagcat
     3481 agtttttctc cacttaaaca agaatttgaa aaaaggtgta ttcttgtgtc tgacaagaat
     3541 cgtaaagaaa ttgaaaaaga actaaatgga aaaattatag atacaccagg acatactgct
     3601 gattcaattt cactactaat tgatgaaggt gttttatttt gtggagattc tgcaatgaat
     3661 ggatttccaa gtactcacaa aattaccata tttgcagaaa gcaaactaga atttattcaa
     3721 tcttggaaaa caatcatcaa tacaaaacct aaaatgattt atcctggaca tggaaaacca
     3781 tttgaataca gtaaattaga acaaaatcta atctatgtaa ataaaataaa tttatatcct
     3841 ctacataatt caaaagatat tcacaggttt ttgtagaaaa tacaaagtaa atttatttga
     3901 ttaattaaaa aatctaacac tttactacgc tacttatttt aataacttaa tcttccacta
     3961 gcttttcata aaattttgtt ttaaaattaa ttttaaagcg catatgttat ttaaatacat
     4021 tagataaaat tcgtcattaa tctacttaaa gcatatttaa ctaacaccac tcaaccagaa
     4081 aattcagaaa actgcattga cacaggaaaa agtttatttt atactttttt taggacatat
     4141 tgtggacacg tcttaaatcg acaaacagaa tttaaattgt aagattaacc acaatactta
     4201 ttttttaatt atatttttaa acattaggga ggatattatg aatgataacg tagttaagaa
     4261 gaaaaacttt tttgacagga cattaaacag aatagaaact gttggtaata aactaccaga
     4321 cccagttaca atatttttag ggttatgtgt acttttactt atactatcta gtctagttgg
     4381 ttctatgggt atatcagtag ttcatcctgg tactggagaa actataaccg ctgtaaattt
     4441 attgactgta gaacagcttc aaattctttt aggaaacata gtcagcaatt tccaagggtt
     4501 tgctcctttg ggtctagttc tagtaactat gataggtgct ggtgtatgtg acaaaacagg
     4561 acttatgaca gcaacaatca aagcatcagt aagcaagatt cctgaaacaa gagtaactct
     4621 tgttgtaatg actataggta tgcttgccaa tatagcttca gatgcaggta caatattatt
     4681 tccaccatta gctgcacttg tatatcttgg tgttggtaga catccactta taggtttatt
     4741 ttctggatat gcagcagtct gtctaggatt tgccgcaaac attatgataa gtgtcaatga
     4801 tatacttgct gcaagtttta cagttccagc agctcaaatg attgattctg gatttcaagc
     4861 caatgcaaca atgaacttat tttttatgat agcatcaact tttgttctaa tacttttggc
     4921 aacatgggta acagaaaaaa ttgtagctcc tagatttggt aaatatgaag gaaatgcaga
     4981 actagatgtt gatggaagtc ttagtgatgt agaaaagaaa gggcttaaaa aagctggtat
     5041 aagtatatta atatatgtgg ctattatagt agctcttagt gtaatcggta aaaaaccatt
     5101 cttagctgac ccagaaacag gagatttact atcaaacaat gctcctctta tgaaaggtat
     5161 ggtacctata atagcactag catttttcat acctggtgtt gtatacggaa aaactatagg
     5221 taaaataaag aaggataagg atgtagtctc tataatggct agtgcaatga gtgatatggg
     5281 tggatatata atactagcat ttgctgcttc acaatttttg caattattca caaatagtaa
     5341 tttaggtctg atactagctg taaagggcgg agaattttta aaatcagcag gaataaatgg
     5401 acctttattg ttgataggat ttattatatt atcatgtttt ataaatttat ttataggtag
     5461 tgcatctgca aagtgggcta ttcttgcacc tatatttgta ccaatgttta tgatggttgg
     5521 atacaatcca gcacttactc agatggctta tcgtataggt gattgtataa caaatccact
     5581 tagcccatta tttccgtact tccctattat actagcattc acaagaaaat acgataaaga
     5641 tgctggtatg ggtactgtaa ttgctaatat gataccatac tctatatcat tcttaatagt
     5701 gtggactata ctattaatat tgtttatggt atttaatatt ccactaggac ctggaatact
     5761 tccttcatat agcatgtaat ttatttaaat ctttctaaaa ggagaaataa tttatgttag
     5821 ataaaaatag acttataaat aattttatgg atatggttag aattgatagc ccatctaatc
     5881 aagaattaga aatgtctaag tggttggtaa actacttaaa agaaagaaat atagacgcca
     5941 ttatagatga tgcaggtgaa aaatatggtg gtaatacagg taatgtcatt gcctatataa
     6001 aaggtgaaga aggaagtaga ccactttgtt tatgtgcaca catggaccaa gttcaacctt
     6061 gtttaggtgt aaaaccaata ctagatggta atgtagttag aagtgatgga actactacac
     6121 ttggtgcaga tgataaagct ggaatagctg ctatacttga agctttagaa catgttatta
     6181 cagaaaaaat accacataga gatatttatc tttgttttac aatctgtgag gaagccggta
     6241 tgcatggtgt caaaaacttt aatcctgaca acttgccttg taaagacatg gtgattttag
     6301 attctggtgg agctattggt tcgatagcat acaaagctcc tgctcaacaa tctataaaaa
     6361 tctcttttca tggaaagaaa gcacatgctg gtatagaacc tgaaaaaggg ttaaatgcta
     6421 tattagtagc ctctcatgca ataagcaaca tgcacatagg cagaatagat tcattgacta
     6481 cttcaaatat aggtaaaata gaaggtggtg gagctacaaa catagtaact gataaggtta
     6541 cattgactgc tgaaattagg tctcatatac ctgaaacttt agagtatgag cttaatcata
     6601 tggaaaaatg ttgtaaggat gctgcatcta aatttaatac tacttatact tttgaacata
     6661 gtatgtctta ccctagtttt gaacttagta gagatagtca tgttttcaag ttaagtgaag
     6721 aagcaataag acaagttggt gttgttccaa atccaatggt aattggtggt ggaagtgacg
     6781 caaatatatt agctaatctt ggatataatt gtgctatatt aagtctaggt atgtatgatg
     6841 tacacacagt taatgaatat gtaaatatag acgaattata tgatactgcc aaaatagttt
     6901 atcatatgat taaattataa atatcataat ataactcttt aaattataaa aataaaaacc
     6961 tatgtatctt caactttata agttatatat taaagttgtt ttacataggt tttagtattt
     7021 tatataataa tttgttttaa catttttcat aataagcttc aatattttgc ataataattt
     7081 gttctagcat ttttaataac aagctttagt attttatatt aataatttat tctagtattt
     7141 tacataatca tttgttctaa tttttttcat aataagcttt aatactttat atagtaattt
     7201 gttgtagaat tttatacaat ataatgattt atacagtttt ataaaatatc ttaaaacagt
     7261 atttcagtaa tattttttgg tcttcctcca ccaatactag tctcttttgc acatattcga
     7321 cctaaacctg catttactat tttatttaat atacgtcttg cacttctttc acttacattt
     7381 agatactctg ctaactcttt tgaatcataa atcttacttt ttctattctc atttatagca
     7441 ataatttttg caactgactc acaactcatt ccagtctttt tagatatttc gattaccttt
     7501 tcatcagata gcatcaaaga ataatttaat tcattttcgg aagctaaagg tcctcttatg
     7561 ttttcatctt catctaccaa atatatacca atttcttttg aatctatgga gtgtcttaat
     7621 gctttatgtg cattattttc agcctgatac gcagttactc cagtaccaat tcctatacta
     7681 agagaaaagc ccatgtcttt tatctctctc tttagattga atagcttatc ataattatac
     7741 tcattatcag ctaccccttt atgaacaaat acaacataat catttcttcc aagtaaaaat
     7801 aaggaacctt gaatacttct tacatagttt actacaagct tatccatatc tgattttttt
     7861 atcatgtcag aataatagtt atctatcttc tctttataat ctattaaact caatatttca
     7921 acagcaattt gagaaaatct tgccttactt agagcatatt cactctttac cttgtcataa
     7981 ctttctttga ttaattgtat agtagcttgt aaacgaaata caggatagcc tctttctttt
     8041 aattcattat atactgcacc aaaaccagtt aacattatat ccgtcttatt ttcttcaaat
     8101 aattttatat gccattctac aagttcagac tctcctactt ccaaagaaaa tggattacaa
     8161 aatacactcg ttgctttaat atcaaattct ttaagtgcat cttccacaac atatctatca
     8221 actacatcta tactgaattt atctaatttt atatttgcac tttttattgc ccaaaatgcc
     8281 ttcattatac tcgtcccact tctcggtaca aatgaatgtg gcttacttat ctcatactta
     8341 gattttataa attcataaac tgcacatcct gtaaatataa tagcatcaca ttcgtcttca
     8401 catttagaaa taacttctat tgtatctact actttttctc ttacatatat tttagtcttt
     8461 aaatcactgt ctatctgttt taaatcatcc tttatcttaa acccagagtc tgaaggacct
     8521 ataataccca ctatcataaa cttcgctcct aatccataaa tattatactt tttaaaaatt
     8581 atatcctcac tattttctac ttctatttaa ttattttgaa ctactccatc ctacctacat
     8641 ttagaatgga ataattagct tatttttgat aaagtaactt ctcaaaatca gaacttggaa
     8701 taggcttaca ataataataa ccttgaatta ccacacaatc taaactcact aaaaattcag
     8761 cttgttcttt tgtctctaca ccttctgcca ctacatccat atctaaatct tttgccatgg
     8821 ctatagtgtg ctttaaaact attttttctc tgtctgtgat tccttcctca ttaaagaaac
     8881 cacaatctat ctttaatgta tcaatattaa tattttttaa catatttaat gaagaatatg
     8941 ctgaaccaaa gtcatccata tttaaaacaa taccatccat tctaaggtcg gctattatct
     9001 tagtaaataa ttttatattt ttaataaata aactttctgt aagctctaat tctaaaaaag
     9061 attttggtat actatattta tttattaaac ttaaaacaat ttctttaaaa tgtggattat
     9121 gcaaatgtaa tcttgataca tttacagaga taggaactgg ctcttcacct aaatctatcc
     9181 attttctaat caaagaacat acttgttccc atacatacat atctaatctg ataataaacc
     9241 cattcttctc aaatagtgaa ataaatttaa ttggaggtat tatacctttt tctggatgaa
     9301 cccatctaac caatgcttct gcaccaacaa ttttatttgc attaagatta tacttaggtt
     9361 gtaaatatac atggaattgc ccattttcaa gtgcgtattc catttccctt tccatctgtt
     9421 tttcttcaag tgcatcattt cttatagtat catcataaaa tatatagttg ttgatacttt
     9481 gtttctttac ttttttgtga gcatatcctg ccatttcaca cgcatttgta attggaatat
     9541 gattatccaa aattctataa accccaaaac atggtactaa catatattca aatttttttc
     9601 tatttaagct atttttaatc ttataaatta ctttatgaat atctctatca gagtaactct
     9661 cataaaaaat gcaaaataca tctgcataca tacgacaaca tataccatta gaaccaatgt
     9721 ttttttgaat caactctgca acatatatta aagctttatc tcctcctcta actccataaa
     9781 tgtcattaat taccttgaac ttatctatat ccatcattat aattgcataa cgcttgtcag
     9841 gatatctttt aagcattaaa cttatttgtt cacaaaaact ttcataattg tatagtcctg
     9901 ttaatgtatc tttttggttt ttatgtagtt gcataagata atctatattt tttatagcaa
     9961 ctaaaatatg tgtttgtttc tcacctaact tatttttact actaatcttt aacatataaa
    10021 ttaatgccca atagtacttt ttatctattc ctaaacgtct atactcaaaa tttagttcta
    10081 aatcgtcttg cttgctatta ttaagactat caagagattt tagaaggtgg ttatcattaa
    10141 ataatattat atcatcagga tgaattaaat atttttttat gtatgaaaaa gttttttcaa
    10201 gattttcctt tataggaaaa ctaaaaatat tgctctctga ttgataaatt ggagtaatta
    10261 aattcgtatc aatgcaaata gataaaatta tatcatatgt attataagct aatttttcat
    10321 aagctttaga ataccctttt atcattaaat catcaaaata aaccttatca actaactctt
    10381 tatgtttaaa taaaatatta ttcgtcgaat cagctaagtc actaattact ttcatttcat
    10441 tatttctaag tttgttctta ttataatacc tcttcttatc ttggtacatt ctctcatcag
    10501 caactttaat tagcttatgt atatcaatat tatcgtcact ccaaatactg cccactgcta
    10561 cactatatat catctcatcc ttaaattctt cacttaacca actcactaat tcttcaaata
    10621 gttgttttga tatatcttca cataatacaa caaactcatc tcctccaata cggtaaacat
    10681 tataagaatg aaagtatttt tttattatta cacatgctga cacaatagca gaatcgccat
    10741 tagcatgtcc aaagttatca ttaatatctt ttaacccatt tatatctaca aatacaactc
    10801 ctatggattt aacttttcct ttttgaaaat tatctatata cttaatatat ttatttctat
    10861 tatatgcacc agtcaagaag tcatagtagc tcatataatt taatttattt tctacttttc
    10921 tttttttcat ctcagttaat aaaaaataag atagggatgt caaaagagag gtatctccaa
    10981 cagctttatg tggattatca actccaataa accctactac tttatcatct tgttttagtg
    11041 aagatgtcat taaactttca attttttgag gttttaaaat ttcatattct attggctgtt
    11101 cttcttttat attttctata tcatcaatta ttacaaaacc tttttgttcg aataataaaa
    11161 accaattctt taattcatta atatcaagat ttgttagtgc ctgtttttgt tcatcaacac
    11221 cttcattgca ccattcatat gtattagtta cttttgttct atcttctgat acttcaaaaa
    11281 catatgctct atcagcttta tgaaatttac caatattggt taatacaaga tttacagcat
    11341 cagaaaaatt agattctgtt ataagatgtt taatacatcc aactaataac tcctttatct
    11401 ctaatttttc tgctattgac ttacttatat cttccttttc tgtaatatca agagctattt
    11461 ctaatcgtgc atgtttgccc tcccatttaa ttaacttgtc ctttaatata aaatgtctat
    11521 ttgttcgagt attcgtattt tcccatatgt aatattcatc ttcttttaaa atagagtttg
    11581 tacaaaattc gcagggagaa tctttcccct gtaatgcttt ataacatttt aaatgggata
    11641 tatcattaag tcccattgcc tttttcccaa tttcattaat atataaaagt tcatatgtat
    11701 ctaaagaaga aacatataca atttcatcca actcatctat taaatttatt acatccattt
    11761 atatatatct ccttttattc attttccaga ttgaattttt aaagctttta aaattttgct
    11821 atatatttgg caatgatatt atacttcttt atgttttaaa atagtacctt aatgaggttc
    11881 tattttttag taaatagaat tattattgtg tcaatcgtac aatagagttt tgtaatactt
    11941 aatttaatat atttataatt aactatctta ttatgtattt acaattaatt ctcatataaa
    12001 tgacaaatct ccacctcaat taagtattcc aatcatatat gaggataata cttcattcat
    12061 ttaaaaatat aaaactatta tatattaaaa tttcttcaat aataaattat ttagattgtt
    12121 actttgatat attatataat atcacaaagg ctgatataaa aagacaaaaa gggcaatttt
    12181 tttatatcaa aaaaaatgtt aaattgatat tatccatata taaattgata ttatccatat
    12241 aggtgctata tttaacaatt ctacttactt cccttacact tattagcata tagttattaa
    12301 gagtttgttg caaaacaaca cttagcacac tttaaaaagt gtggtctaat aagtaatcaa
    12361 cgaagaagac ataataaagc ataaaatagg atatactttt ccacgtcatc aaaattgagg
    12421 attttattat taatatatga taaaatatgc ttaataaatt aaattttaat gggagaatag
    12481 ttatgaaaat atttagtttc ttgttgtttg tagtgggaat atgtttttgt attgctggat
    12541 ttatggggca ttcctcagct ttttctggtg caacattgag tttttttgca actacaatga
    12601 tagctgtaag acaagtcaaa actaagaaaa aatgaaatag gcaaatccta actttataag
    12661 ttaatattac ataagtaatt agtctaaaat tagatacttt ttctattaca aaaatacatc
    12721 atccactttg tattctctta aaaaaaataa aatagctatg taaattcaag attagataga
    12781 ctaactctct atccatttca ttgtaaaata tacatagcta tttgtattcg atttaaaata
    12841 cttagatttt cttacataac aaataatttc tatctgatta ttactcatgt tcatttatta
    12901 accactcagc aactaatcat ttagtatcta ttcatccgcc atacggtaac ctaccccaac
    12961 ctcagtcaaa atatattctg gctgagcagg attcttttca attttccttc ttatacttgc
    13021 cataaaaact ctaagcgact gattttcact ttcatagtaa tttccccaaa ctttatctat
    13081 tataaactta tgggttaaga ctcttcctga atacttagac atcaactcaa taatcttata
    13141 ttcgattgga gttaaatgta tttcttcacc attaattgac acaatatgat tttcataatc
    13201 tatctttagg tttttaactt caaaagactt tggagcatca ttatttatat tatcctgttg
    13261 agctttattt ctaagagcca cacgtactct agccaaaagc tctgttacac taaaaggttt
    13321 agttaaataa tcgtctgctc ctccatctaa agcttccacc ttatctctct catgctctct
    13381 cgctgaaaca acaattattt tattagtctt tgcacatgcc ctaactcgtc ctattacctc
    13441 aatcccatca atatctggta gtcctaaatc caaaattatc aaatctgggt caagagaatg
    13501 tataagtgat atagctgttc ctccattatc tgtctctaca cacctataat tttgagtttc
    13561 aagagatact tttataaatt tacaaattgg cttatcatct tctacaatta gaactaatgg
    13621 ctttatttcc acttgtcttt cccctcctca tcatacatag gtatgctaaa tctaaatgta
    13681 gctcctccag aaggattatt aaaggcgaca atttctccac catgagcttc tactatagat
    13741 ttacatatct ccaatccaag accaatacct cttctttcaa gatttctaga cttattctta
    13801 gtataaaacc tattaaaaat attcccgata tcttcatttg gtattccttt tccattatct
    13861 gcaacttcaa atagtacttg atttgactct ttttttacag ttagttctat cttagaattt
    13921 ttaggtgtat gccttatagc attatcaatt agatttacta acacttgttc tattaataat
    13981 acatccacat gaacgattat tatctcatct ggaatatttg tttttatgtc tctactattg
    14041 gcaaactttt tgactcttaa tatagattca gctattattt catcaacaac ctctggtcgt
    14101 ttttcaatat ccaattttcc ttcatctatt ctggtcatac taagtatatt ctcaacagaa
    14161 tgagttagcc aactcgtatc ttcatatata tttttcaata attctactct agtttcttta
    14221 tctatcacat catcattttc aagtattgtt gaagatgaac ccaatatact tgtaagtgga
    14281 gttcttaaat catgagaaat tgacctaagc aagtttcctc ttaacctctc tctctctgcc
    14341 tcaaggtttg cttttttatt tttctcaaat aaattttctc tatcaattgc caacgcaacc
    14401 tgtgttgaaa ctgcttctaa tagtatcttt tcattttctg taataatatc tatcttaaaa
    14461 cacgcaacac ctataacacc aagaacattt gattcttgac cttttatagg gaaataatat
    14521 gcactgctgt ttttacaata ttttgtccca attccaactg ggttacctgt cctaaaagct
    14581 tcttttatag cttccttttc taatactgaa tggaatatat tctcacttcc atcattgtta
    14641 caaatataaa tacttggctt acctagatta ttattctcat ctgcaatagt cactattaca
    14701 gttctattta atatatttac taaattagac ccacaaaatt caataatttg atttttattt
    14761 tttgctttaa gcaacgtttt gttattctca tagagcaatc ttatcatttt ttctctctta
    14821 tgagaaagtc tcacttctct ttttatccta gaagttaaag tacttgttat aattgctgcc
    14881 attagcataa tacaaaaggt cataggataa tcttttctat aagctaaaaa agtatataat
    14941 ggatgcgtaa agaaaaaatt aaatgatagc actcctatta ctgaagaaag tattccaaaa
    15001 aaatatccat aggttatact tgtagcaaat agtacaccta atatgtatac tagtataata
    15061 ttagattcat taaaacctac cttttcaaaa actactgata ttccagtagc aattgtcata
    15121 accaatatag tttttatgaa gtccttaata attatctttt tactgagata gccatcttca
    15181 tcataatcat actcttttaa gtcttcatgt tgtagtcgtt cctttttcat ttactacaat
    15241 tcctccttat tgttactgtt taaattggct ctattttata taatacaatt ttatcataat
    15301 aaattcaatc aatacattta attaatgtac ttaaattact aaaaggtgct gtcttaaaat
    15361 gaaaataaat ctctatttca aaacagcacc tatatattta aattactaat ttagttttta
    15421 catacttaaa tcaatagttt tattcaattt cataaattat aatacttcaa ttataaaaca
    15481 tttctatcca acaagcataa atataaagtg ggcacatata cctgcacata aaccacctat
    15541 agtatgcgaa agtatcgctt taccagccaa tggtcttgca tctaaagcat ccatcatacc
    15601 tatgtgtgta cttaaatatc cactccaaca catacccatt gctgtgaata ctgctatatc
    15661 attaggagtt atagcgcctg attttataaa ctctggtact aaagatattg ctgctccaac
    15721 agctcctaat gcagttacag ggaatgctat tgcctctggt gatgtgaatc caaataatgg
    15781 ttctattatg aagctaatct tatcaccaat agcaggtaat aattttatac cttcatatgc
    15841 agctcctgtg taaacagctt gacctgtagc agggtctgta gatggtccaa aagttaataa
    15901 cataactaat gtacaaacta ctaaaactcc tggtattata gccataccca tttcaacacc
    15961 taacttacca ccttcaagta tagcatccaa tgttctttgg aatacattac catcccttat
    16021 ttctctaaac tcttcaagtt ttgcaccttt atcatttatt tgcttttcag tatcattttt
    16081 agggtcataa ttataatact ttttagtaaa agtcaacata attctaacac taataatact
    16141 acctattatt gccccaacat tacctattat agctggtagt acatattctt taccttgagc
    16201 tatcataaaa gttgttacaa taagtcccat accaaaggct gttcctaaat tacataaagc
    16261 aggtacttga tacttcttaa aatattgtgt aaatgttttg tctttagcaa atggtataat
    16321 agctggatta tcagataaat atgttgcaac tactcctgct atactagcac caggtaagcc
    16381 ccatataggt ctcataagac ccttaaatat cttattaact aaagatatta ctccaaactc
    16441 tgaaagcaat gcactaagtg cacctgcaag aactgccata gccattatta aaaatactgt
    16501 gtctaataaa agcgcatgtg ctgtactcat tattacttta aacatcattc ctgcacccat
    16561 gatgcttcct acatatccaa atcctaccgc caatagtaca ataaatacga aggtttccat
    16621 acttactgcc ttgactcttc tcttatccga cagcttttcc atcttgaaat cttccccctt
    16681 ttaattttaa attaaggttc ctaacattgg tacaaatttt ttggtacaaa ctgagttcta
    16741 aaacttacac tcgtaatgca agttgtcagt tttttgaaat aaaaaaaaat tacgtttata
    16801 gaataagaat atcctcttta atttggatac tcttattcta ttttatgtta ttatattatc
    16861 atccttttgt ttaaaagtca attttttcta tttttgttgt ttaattttat ttatactctt
    16921 tttatatttt tcattttctt ttatctttgt aagtactcca tataagttga cttttctaaa
    16981 tacagtagtt ataaatacca atcctaatgc aattgaactt gcacacgata atgtactcac
    17041 cccatattct acagctttta tataattatt ggtcaaaaca gaatacatag tattatatat
    17101 tccataacct ggcactaata ttattaaaca gcaaacactt aatgtagttg ctggagtcat
    17161 ataaattctt gcacataact cacaatatat cgcaaaacaa actgtagcta taaaaaaaca
    17221 taagttttct gttactccca tatttaaaag aaatttatac gaaaaccaag ctatacttcc
    17281 accgattcct gctagtatta agttttttcc tttcatatta aatagcactc caaagcttaa
    17341 ggcagtaaaa aaagctgcta ttacttctat aaacaaatgc acttatatcc cccctaactt
    17401 gaagtacaat ccaagtacca agaacattcc tactgctata gatacaccta caaaaaatac
    17461 ttctgctaat tttgcaagac cagaaatcaa ctgtccatct aacaaatctc ttaatgcatt
    17521 agtcaaagca agcccgggaa ctaagagcat tattgctcct gctattaaat ggtctaacgt
    17581 ctttatgaat ccgagcttta aaattaaaat tgaacaagaa gctataataa ctgctccaat
    17641 tgcatttaca aaaaagtcat taagggaaga tttacttaaa tacataataa caaacttagt
    17701 cagaatccca acagtaaatg cagctataaa ttcttccaca tttcctccaa ataatatcga
    17761 aaaagttgct gctgcaatac ctgcaaaaaa tattgttatt aaatccgagt aatagttatc
    17821 ttcacttatt tcatctaaaa ttttctcaca taattcaata tctaaattat ttatacttat
    17881 tgctcttgat aatgagttaa cttcatgaac caagtttagg tctactcttc ttgagcttat
    17941 tcttttaatt gctgaatgta atttcccatc tacaaataca gatgccatta cagctgttgg
    18001 agttgcaaat acatctacgt ggtctattcc aaaagattga catattctag ctatcgtttc
    18061 ttcaactcta taggtttctc ctccactttg caacatagct ttacctgcat ttgaggaaaa
    18121 gtttaaaatt ctatctatat ccacaaaaca tatctcccat tcttttatag tattattcat
    18181 attttaatac aagatattat ttatttaaac tattttaaat aattcatatc caacatttac
    18241 attttatgaa aaaacttgtt ttttatgctt gtcaaaatca aattttaagc ttgtttacac
    18301 cataaaatgg ctgaattaat atttgacaaa cactttcaac catttatata accttctaaa
    18361 ttgtataact ttttactaca aataaaatta tatctctttt aattctttct cttgagtatt
    18421 tagcttatag taacttcata tttatatatg tacaatataa aacattattt ttatgataca
    18481 aaatataatt tgtaaatgta tatatgtatt aactgtgtat atataaatta ttcttaacct
    18541 tatcagtttt ttcatatatc ttcatctata ccgaccatat attaattaca ttgaccatat
    18601 attaataaat gatattacat agacttaagt atattaaaaa acttaaattt agttatttaa
    18661 caacataata ctctaattta atctataaaa atccatacct aatataatgc aactcttatt
    18721 ttaacaccat ctattacaca aaacaacata ttaaaaagta aaatacaaaa tatctactat
    18781 aaaccttatt ttaatactga atatatatta taatatattt aatgtagctt aaatatagtt
    18841 taaacataaa gaaaggaaac ggtatatgct aagaaaagat aaagttctat tatctctaca
    18901 taaattatgt gaaaatatga ctttaaaaga ccttcaatca gataaaacag gattcactac
    18961 aatagaaatt gctaaccatt catctcttga tagaagtaat accagtaaag aattaaataa
    19021 cctatacaat gaaaaaaaag ttctgaaaat atctggtaag ccaattttat ttctacatgc
    19081 tagtattata gaaaatttaa ttggccgtac tttgaacagt gatgaatttt ctataaataa
    19141 ttgtaatgag ctacttaaat caagtaaaaa tgactcagaa gatatttttt caacattact
    19201 tggacatgat tctagtctta aactacctat agaaaaagct aaatctgcta tactctatcc
    19261 tccaaaaggt cttaatacat tactaatagg acctactggt gttggaaaaa gtacatttgc
    19321 tgaaacaatg tacaaatatg ctgtagaaag taaaatcttt tcttctgaaa gcaaattcgt
    19381 agtatttaac tgtgctgaat atgcagaaaa tgctaactta ttattaagta atttatttgg
    19441 ttatgtcaaa ggttctttta ctggtgctaa taaggataaa ttcggcatag ttgcacaggc
    19501 tgatggaggc atattattct tagatgaaat acacagatta ccaccagaag gtcaagagat
    19561 gctttttctt ttgatggaca aaggcatata cagacgatta ggagaatctg acattgtgca
    19621 taaagctaat gtctttattg tgtgtgccac aacagaagat atacattctt ctctgcttgg
    19681 aactttttta agaagaatcc ctgttacaat caacctacct gctttacagg ataggtcttt
    19741 aaaagaaaga ttatctctta ttaaatactt ctttattata gaatcaaaat acaccaatgc
    19801 aactataaaa gttcacaaag atgtaattaa atcattactc ctttatgatt gttttggtaa
    19861 tataggtcag cttaaatctg acatacaact tatgagtgca agagccttct taaattacaa
    19921 aactggactt aaggataata tagaaataga tttatccctt gttcctgatt acatctatat
    19981 tggtctttta aaattgaatg aaaatcgtag taatctaaat aaattatcag aacttgatta
    20041 tatgcttttt tatgaattta atggtaataa cttagaagtt gaatatacaa aagaactatc
    20101 taaatacaat atagatacat caaacaatgg atttaaatac tctacagaga ttccttatct
    20161 ggaaaatgat ataaggagta tgtttataaa ttacatggaa aagtactcta caaaattact
    20221 ttcgatatct tcagtaactc attctgatta tacaagtaat gagattttta aagtaatcaa
    20281 tccaaagatt tactatgctg ttgaacattc tttaaaagag gctgaaaaaa tatcgagaaa
    20341 caaatacaca aaacaaactt atattgctct ttcaatgcat ataagtgcac ttatagaaaa
    20401 tataaatcaa gacacatata atcctaagta caatttatat aatgataaaa ttttcttaga
    20461 taaagattta aatatagcta aaatagtttt aagtataatt gaaaatgact tacaaacatc
    20521 tttacctaaa gatgaagtaa aatttatatc catgtttcta aatagcatgt atcttgaacc
    20581 aaacaatcaa tctgaaagta taggagtcat aatacttgct catggaaatt caacagcaag
    20641 cagtatgtgt gatgtagcaa attcattgct tggtacaaac cattgtaatg caattgacat
    20701 gcctcttgat gtaaaagtaa gtaccatact tgataaaact atagagttgg taaaagaatg
    20761 tgacgaaggt aagggtgtac ttcttttaac agatatgggc tctcttatag cttttggaga
    20821 acttatttca gaaaaaacaa aaatcaaaac tcgctcaatt gaaatggtat ctactccaat
    20881 agttttagaa gcagtaagaa aaagtacact accagaaatg actttggatg aattagctaa
    20941 ttccttaagc tctttaaatc catatattgg acgagcagca atcgattcaa cagaaataaa
    21001 ctatcctgaa aagtcaagag agtatgtaat aataactact tgtataacag gatatggttc
    21061 agctatgaaa gtagcagact ttataaattc ttcacttaaa aatattagta aatacaatat
    21121 taatttagtt cctcataata tagaaagttt taaaatgtat aagaataaat ttagtaatac
    21181 taaaattctg gcagttgttg gaagtgtaga ttttaatgta gctaacaccc cttttataca
    21241 catagaggaa ctactaaatg gatatggcct agatatgcta aacaaaataa tagaaggtac
    21301 attcaatgcc aatagtatta caaatataaa agaaacaaat ctatcataca acagtttaat
    21361 aataaacttt atgacccaaa acttagagct tttggactct gttaaattat tcaactatgt
    21421 caattcttcc ttatttacaa taatagaatt aatagatgaa aacttaaaga gcaaatttaa
    21481 gctaggatat atactacatt gctcttttat gatagaaaga tgttttaaaa atacaacttt
    21541 tccatataat aacattactg aattaattag taaaaatcat gaactatata gtttaatacg
    21601 tgaagccttt aagccaacag aagcttattt taatattttg ataccagacg aagaaattgc
    21661 ttatataata gacatgatta atgagtatct aaataaataa aatttatagc acttattttg
    21721 tgcatattaa ttaatatatg aaatggagga cgagattttg aaaggaaaaa taaaacagtt
    21781 aacaatactt gcattaattt ttatatttat aactcctgta tttgcatttg cagatactcc
    21841 accagtacca aattcttcaa gagcagcact acttatagac caggaaacta aaagaatatt
    21901 atttgaaaaa aacattgatg aaaaaatgcc attagcgagt ttaagtaaaa tgatgacctt
    21961 tttattggca atagaagcag ttgataaaaa tcaagttaaa gagactgata tggtgaaaat
    22021 agacaagtct acagcttcag taggaggttc tacttgtaaa cttaaagatg gtgatgaaat
    22081 atctctaggt gagcttatgc agggtctaat gctagtatct ggaaatgatg ctgctatagc
    22141 catagctaaa catattggta aaactgaaaa gaattttgta aatatgatga acaagaaagc
    22201 agaagaaata ggaatgattg atacgtatta ttttaatcca aatgggcttc ctatatatac
    22261 tgacccagaa cataaagaac ctcctattga aaatatgtca actgctcacg atatagttac
    22321 acttggaaag tatatgtacg accattatga aaatcaagtt acaagaataa ccactatgca
    22381 agtatataat gatactaaaa aggattttac acattataac acaaatcctc tgcttgtttc
    22441 agtacctggt gttgatggaa taaagacagg atatacagat aatgctggat attgccttgc
    22501 attttctatg atggtaccta aagatgctaa aaacgaaaga aatcatagat tgataggtgt
    22561 tgtacttggt gatggtaaca agaaaaatag aatttcttct tccgctactt tattgaaata
    22621 tggtaaagat aatttccatt ctaaaaagat agcacataag ggtgatatta tagagactcc
    22681 ttgtgttgat ggtattgatg attttaagat aacagttaag gttgataaag atttgtatgg
    22741 tgttgtttca gataacgaaa atatcaatcc taaggtagtg tttaaaaata tgaattatcc
    22801 tatccataag ggagatattg ttggtgttgt aaagtattat aatgactctg gtaaatttgt
    22861 tggaagtgtt gatgtaaaaa gtgaaagtaa tataggatgt attcctttga aggataagat
    22921 taagattaag gttgctaaga taaataaaaa acttgagatt aaaaattctg tttgttttaa
    22981 agcatagtaa agcatattat taaaagggta tgttaaattt ataaagactc cactttgtgg
    23041 agtctttttg ttttttacaa aaaattaaaa tagtggcaac aattgaggat gtgagggaga
    23101 gttgccacta ttttgtatag ggtattatat tatgttttta agctagtttt actatcttct
    23161 atgtttatat aataactctt gtgtgtgaat ttaattgata attaatatga aaattatgtg
    23221 aagttttaag ttagttgact tagactattg taaatttttt cagaaaaatt ctgttcataa
    23281 gtctattaga ccaccgaatt atttacctat aatattccga tcaaaaaatc tattcttttt
    23341 taaaagtaag gtcatgatta ttgtacatat ataaaaattc cgaaaattaa tctacacact
    23401 agtaacatta agtataataa atttatttta gtaaaactat taaaataacc tcataagaaa
    23461 ctatagatta aatcaatcaa tctaatctac agtttcttga ttattcctta atatttataa
    23521 tttacattat ttgtttattt taaaactatc tgttacaaat ctagtcaatg ttgaaaataa
    23581 attcgctctg aatatagtag atgatggttc taatgcagtt atctcacctt gtacttttgc
    23641 tatatcttca tatcc
//
LOCUS       DAMJVI010000056        23269 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.058, whole genome shotgun sequence.
ACCESSION   DAMJVI010000056 DAMJVI010000000
VERSION     DAMJVI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23269
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.058"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            239..1471
                     /locus_tag="PC545_002245"
     CDS             239..1471
                     /locus_tag="PC545_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2457012.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYILMKCFGIKDK"
     gene            1485..2291
                     /locus_tag="PC545_002246"
     CDS             1485..2291
                     /locus_tag="PC545_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HDF2457013.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATGYNLESLGGVKTLELIREHNKYIEESMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFDRKGKIMGEYCKNHLWSLEAVYFKGGEKVEVYDADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSSYNI"
     gene            complement(2403..3272)
                     /locus_tag="PC545_002247"
     CDS             complement(2403..3272)
                     /locus_tag="PC545_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457014.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENSGEVKILSPAAAN
                     VLPSLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3644..5071
                     /locus_tag="PC545_002248"
     CDS             3644..5071
                     /locus_tag="PC545_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HDF2457015.1"
                     /translation="MKLTKSISSMLCLSLVLSLSPVAYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSSSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEGLKDISE
                     IAVVNGYTGLADAVSIGSVAATNGMVILPVSDASGISSFKDFIESKNINKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKNQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNATSITQVGSGGNENAFKELKDLQANS
                     NNKRVMYVTNTDKVNIRNDATIEASVIGYLNNGDEVEVLDVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5148..5417)
                     /locus_tag="PC545_002249"
     CDS             complement(5148..5417)
                     /locus_tag="PC545_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457016.1"
                     /translation="MKFEYYYLIQDIAGILLAFIGLRMSIIGFRILSMRGLSINTLLI
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMLIISIIINPRIKVSK"
     gene            complement(5420..6862)
                     /gene="nhaC"
                     /locus_tag="PC545_002250"
     CDS             complement(5420..6862)
                     /gene="nhaC"
                     /locus_tag="PC545_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDF2457017.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICIVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLITKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLEFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDDEIEELEIFDTAQRRVD"
     gene            complement(6887..8053)
                     /locus_tag="PC545_002251"
     CDS             complement(6887..8053)
                     /locus_tag="PC545_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2457018.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRLEQEIYGYTTRPDSYNESIVNWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKANDKIMIQEPVYSPFNSVVKNNNRELIISPLQKLENGNYIMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPMAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKYYKLLDDAFTRIDIKRNNCFSLV
                     ATEASYNNGESWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDEELESILVQKGKVALNQGNSFGIGGSGYQRINLACPRSMLEEALIRIKNAIN"
     gene            complement(8278..10398)
                     /locus_tag="PC545_002252"
     CDS             complement(8278..10398)
                     /locus_tag="PC545_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457019.1"
                     /translation="MKQMELAIVSLKKDAGEIYENQIRQFLGDNLKINLYSFEEGNLK
                     FFKEKLILLSAYLKYDEIVKLSHYDAQIIVPKLTFEKNSIDMISKLEKDKIIYVYNLS
                     KDMAIETISLIHRLGIDNINILPCYPEIEFTPTDAVILTPGEKILPKFKNCEVIDLKY
                     RIIDLSCIVEIATKTKLKHLIKDDLIKKYVEKIIPTSYSTGELLLDANKFERQFDLLL
                     SIIDDGIICTNNDGIIQFYNHMARKILSINANEMIDSFVGDCIKDINFQSILTNKTPF
                     FEKLIKINHIDINLEIKHIQLNVFDGFILKMTKFSQLEKKQAKLRAQLVNSGNISKYT
                     FDDILGSSIQTINTKKIANKMAQSNSSILIIGESGTGKELFAQSIHSASRRKDGPFVA
                     VNCSTFQENLLQSELFGYDEGAFTGAKKGGKIGLFELANNGTIFLDEIGEMDLNSQSK
                     LLRVIQEKQVRRIGSNNVIDIDVRIIAATNRNLKELVSKNMFRRDLYFRLNVLPLKIH
                     PLRERPADIFEIFGSLKYDIPCNFILSEEVKEIFKMYRWEGNVRELRNLGEYFCYLEK
                     DIIEICDLPEYILDTIDSNYSRTVCNKVSNNIKKYQFNIGKDKNIMKYDYNFKRSLDE
                     YIFILDNLKKAYDLKERIGRKSLCKIALEENRFLTEQQIRNMLLELQDFGLVDILVGR
                     GGSIITSKGVEFLKNINRSNKLNS"
     gene            10631..11032
                     /locus_tag="PC545_002253"
     CDS             10631..11032
                     /locus_tag="PC545_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457020.1"
                     /translation="MKKCLYCNRDIKSPNNKLAIKYDDNMNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILAFIVIITVLYFKQIGK"
     gene            11075..11368
                     /locus_tag="PC545_002254"
     CDS             11075..11368
                     /locus_tag="PC545_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDF2457021.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            11383..11823
                     /locus_tag="PC545_002255"
     CDS             11383..11823
                     /locus_tag="PC545_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDF2457022.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGMTTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11915..12388)
                     /locus_tag="PC545_002256"
     CDS             complement(11915..12388)
                     /locus_tag="PC545_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2457023.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGVTGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(12388..13176)
                     /locus_tag="PC545_002257"
     CDS             complement(12388..13176)
                     /locus_tag="PC545_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDF2457024.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            13504..14748
                     /locus_tag="PC545_002258"
     CDS             13504..14748
                     /locus_tag="PC545_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2457025.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            15071..16168
                     /locus_tag="PC545_002259"
     CDS             15071..16168
                     /locus_tag="PC545_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDF2457026.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEIIIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDTLLHEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHVNHSTKKNILIMGGVLGLLPKSEQFYKELNSL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKDSDLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDATLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGACRECM"
     gene            16159..16857
                     /locus_tag="PC545_002260"
     CDS             16159..16857
                     /locus_tag="PC545_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2457027.1"
                     /translation="MYVVGLIIVVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIVGLHSLEH
                     RNAWLYSYSYVKKDFIESTNIMKNLGVDVNYYRPPWGHTNIFSNSFVKKYNLKMTLWD
                     VMAEDWEKDSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKIAIPK
                     LKASGLKFVTPERM"
     gene            16860..17924
                     /locus_tag="PC545_002261"
     CDS             16860..17924
                     /locus_tag="PC545_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2457028.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFAMMFIYVACEGINIYLGMKALNQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGIMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVIGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVIISQI
                     EEYVKGAQYIKQNPKLVVQILVITIVQITSMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     VVHVKSSEKVKAVKKVAYVR"
     gene            18107..18811
                     /locus_tag="PC545_002262"
     CDS             18107..18811
                     /locus_tag="PC545_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457029.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LVDSTIDLIILDVVMPVKTGFVACNEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEREPNILKTKELCVNLDTQDVTLNGEPVF
                     LTTIEYSILILMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            18804..20126
                     /locus_tag="PC545_002263"
     CDS             18804..20126
                     /locus_tag="PC545_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457030.1"
                     /translation="MINKIKPSKLGVILLIYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNQLMDNFQTYVNNNGIRADDGDDIAKWNSDNWFVSMKIYKGNKVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIVVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSH
                     TLENQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            20313..21686
                     /locus_tag="PC545_002264"
     CDS             20313..21686
                     /locus_tag="PC545_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457031.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISMAFYTLKRDINKGMFREE
                     LKGFESEEVEAFNGKSKIATILVALGFLADIIAMYVFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFVPVITGLVVTT
                     IVAMFII"
     gene            complement(22058..22996)
                     /gene="pdaA"
                     /locus_tag="PC545_002265"
     CDS             complement(22058..22996)
                     /gene="pdaA"
                     /locus_tag="PC545_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HDF2457032.1"
                     /translation="MFRKVLHYLTLLSISIFFIVGCSNSQNNQNENQNKETQLQEDKE
                     KIDSGKDTSNVIVSDGTDKPSKATTNNDNNKLDVSSLDNTTLDWFYIPNNKHKTPEVN
                     TDIEFKFSDYDALYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTA
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKD
                     MVKFFRPPMGKYSEKSLAMTKNLGYKTVFWSFAYRDWDTDKQPSHEEATQKIMDNLHD
                     GSILLLHAVSKTSTEILNDFISNARKLGYEFELLEY"
BASE COUNT         8573 a         2893 c         3680 g         8123 t
ORIGIN      
        1 agaatatata acaaatttga aataagtcta atatatgact taatagatta aattaaaata
       61 ataaatttgg actatttatc tatttataac ttaaattata tcaaattgat aacaaactag
      121 atattatact tttttgtaga atagaattac aattatataa agaaaaattc tattaatagt
      181 gaaaaaaaag tagaaaaaag tagaaattat tcttttataa agaaagggag ggataatatt
      241 gcaagagaga gaagaattag ttttaaatcc agttccaaaa gaggaaagag ttggatggtt
      301 agctccattg tttaatatgc ttggttctaa catagctata tctgaactaa tggttggagg
      361 aactttaatt ttaggaatga ctctttctaa tatgattatt acatcaatta tagggaactt
      421 aatattagtt gcaattatta tgatacaagg ttatataggt tacaaagaag ggttaaatac
      481 gtatgtatta gctaaggggg catttggaga gataggagga aagtatctta tatctctatt
      541 acttggtata actagttttg gatggtttgg tgtacaagca ggggttgcag gactatcagt
      601 acaaaagata tttccaagtg taaatctaac attagttaca gttatacttg gattatttat
      661 ggttgttttt gcactgtatg gctttaaagc tatggctaaa tttaattact tagttatacc
      721 accacttatg atattaatgg tgtggggagt ttttaaagct ttttctactt atggagttga
      781 agctatttat aattatactc ctcaaacaac tatgagtatg gttgaaggtc ttaatattgt
      841 agtaggtctt gttatagttg gtgcaattat atcacctgac cagttaagat atacgagacg
      901 agtaaaagat atttggatta tcagtttttt aggtctaggg attatttcat tattccaaca
      961 agttgcagct ggtgtaatgt ctatgggagc acctacttgg gatataacag aagtattggc
     1021 taatttaggt tttggatggg tagcgtttgt tattttaata cttgcatctt ggtctacaaa
     1081 cgtatcaaat gcttattctg gtggattagc attaaaaact atttttccaa atgtaaaaag
     1141 aaatgttttg actttagttg caggtctaat aggtacaata atagcagcta ctggtataat
     1201 atttaaattt cagtcatttt tatctttctt gggaatcgca gttcctgcaa tagctggaat
     1261 tatgtggtgt gagtattatt ttatacaagg aagaacttat aagcatagag aaggaataaa
     1321 ttggattgca gtaataagct ggttaatagg ttttgcagcg tcgtattata gttcaaaaat
     1381 aaatttctta atacctccga taaatggaat tgtagtgagt atggttattt attatatatt
     1441 aatgaaatgt tttggaatca aagataaata aagggggcaa ttacatgggt cgtaaggtga
     1501 aaataggtat aattcaacaa cacagtgtac ttggagatgt taaaaaaaat atagaaaagg
     1561 cagtagagat gatagatgac ttaggaaaac aaggggcaga tataatttgt ttaccagaac
     1621 tatttgctac aggatataat ttagagtctt taggtggagt taaaacatta gaattaataa
     1681 gagaacataa taagtacata gaggaatcaa tgtcagaagc tgctaaaaga aataatgtat
     1741 acctgatatc tccatatgga accttagaaa aagggtctac acatgtttat aattcagcag
     1801 ttatatttga tagaaaaggt aagattatgg gagaatactg taaaaatcat ctgtggtcat
     1861 tagaagcagt atactttaaa ggaggggaaa aagtagaggt atatgatgct gattttggta
     1921 gatttggtgt aatgatatgt tatgatgcag gttttccaga agtatccaga gaattgacat
     1981 taaaaggttc tgaaataata tttataccat ctgcatggag aatacaagat gaagatatgt
     2041 gggatttaaa tgtatctcaa agagctttag agaacactgt atatacagta ggtgtaaatt
     2101 tagtaagtaa tgactctaat ttaatactat ttggaaagag taaaatatgc aatcctagag
     2161 gtactgtaat aactcaatta gatacataca gggaaaaata tgtattatca gagattgatt
     2221 tagatgaaat aaaaaagtat agggaagata tcccctattt gaaagacaga aaaagtagct
     2281 ataacatata attctatact ataatacaag ttcaatataa aatgataaaa agaggatgta
     2341 tcaatattaa gattaaattt attatacttt gatatatcct ctttaatatt atcaactttg
     2401 atttaaatat ttttaaaata gttgataaga aagtttttga acaacaacac agctttagat
     2461 tcatagtttt cgctaaacca tgaaagattt aaatatcttt taaattcaat atctttgata
     2521 tgtaacaact taatctcagg gtcttgagat aatccccatg attttccaca tataaatcca
     2581 actccttgaa gtgattttat aagagcataa attgtatatg gtgaatcaga ctcaaatgct
     2641 attttgggtt tgaaattagc tgattcgcac aggatatcaa ttacctctct ataattctca
     2701 cccttcgtta taactataaa gttttcatct gaaacttcgc ttagatatac ttcatccttt
     2761 aatgataaag gatggtttat tgaaactcct aatagtattt cttcgcatgt taaagttatg
     2821 ctattttctg agtttagctt tgtaaatgaa gaagatatgt acaaatcaaa atcacttttt
     2881 ttataagatg atggcaaagt atgtgacaca ttaaaagtaa tattgggata tagttttctg
     2941 aaattactca aaagtgatgg taacacatta gccgctgcag gagataatat ctttacttct
     3001 cctgaatttt ctaaagaagt gtcttgtaac tccttttttg aactttctac taaatctaaa
     3061 atgctatcta ctctttttag aaacaatctt ccatactcat ttaaaacaat atatcttcct
     3121 tttctgtcaa ataactgaac tccaagttct ttttctaagg aagatatagt tttgcttaaa
     3181 gctggctgtg ctatatttaa cttgtgtgat gcttttgtta tgtgctccat tctagcaaca
     3241 gtttgaaaat acttaagatg caataaatcc atagtataaa atacacctct tttaatatga
     3301 cttgagttat atcaatttta taatcaactc cacattatac attataatat aaagtttata
     3361 taataaacta caatatatta aaaagttatt taaattgtta tttatatttt gtacataatc
     3421 taattaaaaa aagtaacttt aaatgattaa ctaaggaaat gttgttacaa actttagtta
     3481 tataaaaaat tcatttgatg agcaaagtac taatatcaag taagaataaa agcttattag
     3541 ataagaaaaa taatatttta taataatgat gttacttaaa ttattatgtt atactatttt
     3601 aaataggaaa aatttagata ctataatttt agggggagtc aagatgaaat taactaaatc
     3661 aatatcatca atgctgtgct tatctctagt tttaagctta tcaccagtag catatgcaaa
     3721 tgaaataagt agaacaacag ttgagaatac tttagtaggt caggatagat atcaaactgc
     3781 tgttgaagta agtaaaaaag gatggagtag tgcaaatgag gctgtagttg taaatagtga
     3841 atctattgta gatgcattag ctgtaacacc atttgctaaa ttaaaaaatg ctcctatatt
     3901 attgacagaa aaaaataatc taaattcaca aactgagaaa gaattaaaaa gattaggagt
     3961 taaaaaagtt tatataattg gtctatctag ttctgtatca aatgatgtgc aaaaacagtt
     4021 agaatctaat aatttaacta ctgatagagt tggtggtagt gatagatacc aaacagcttt
     4081 gagtatagct aaaaaaatag aaggattaaa agatatatca gaaatagctg tagtaaatgg
     4141 gtacacaggt cttgcagatg cagttagtat aggttctgta gctgctacta atggaatggt
     4201 tatacttcca gtatctgatg catctggtat atctagcttt aaagatttta tagaatctaa
     4261 gaatataaat aaatcataca taataggctc tactaatgct gtatcagata aaataaaaca
     4321 aagtttacct aattcagaaa gaataggtgg agcagataga aatgaaacta atggtaaagt
     4381 aatagaaaag ttttatacat ctaataaatt aaacaatgta tttgtggcta aggatggaat
     4441 gaaaaatcaa aatcaactta tagacgctct agcagtagga gtactagcat caaaagaaaa
     4501 ttctcctgta ctaatcgtag gagaacaatt aagtgcaaca caaaagagtg tattttcgaa
     4561 taaaaatgct acctctataa ctcaagttgg tagtggagga aatgaaaatg catttaaaga
     4621 attaaaagat ttacaggcaa atagtaacaa taagagagta atgtatgtaa ctaatacaga
     4681 taaggtaaat ataagaaatg atgctacaat agaagcttct gttataggat atctaaataa
     4741 tggtgatgaa gtagaagttt tagatgtgtt aaaaactgga tgggtaaaaa taaaatacaa
     4801 tgaaggtatt ggatatgtta gtgggtctta tttgacaaat aataagcctg ataatagtaa
     4861 tgaaaatata aaaataaagt atgtaaaaga aaaagatggt ctaaatgtta ggaaaggacc
     4921 aagtacagaa gatgaaaaaa taggtcacct ttcatatgga agcaaagtag aaacaataga
     4981 aatgtttgct actggatggg taaaaataaa gtacaatggt ggatatggtt atgtaagtaa
     5041 tgattactta tctgatacac cagttattta attataagta acaataaaaa aactgcttta
     5101 atttgtaaag cagttttttt ataaaaattt aagtctaata actatttcta ttttgatacc
     5161 ttaatcctgg gatttattat tatggatatt atcaacatac aaacactcca aatccagtgt
     5221 ctaattccaa acttagatat taataaattc agtcctgcta tggtaaataa gcagtattta
     5281 attacaatta aaagtgtatt tatagaaagc cccctcatag aaagtatcct aaatccaata
     5341 atagacattc taagtcctat aaatgccaat aatatacctg caatatcctg aattaagtaa
     5401 tagtattcaa atttcatatt caatcaactc ttctctgagc agtatcaaat atttctaact
     5461 cttcaatttc atcatctgtg tatgtaagca tagtaaaacc agttacacca taaataaatg
     5521 aaattatagg tgtaatccaa cttaagaaac aatatggaat aaattctagt ggacttacac
     5581 caagagtctg agcacagaaa acagcatttg aattccatgg tattaatgga ccaccaagtg
     5641 tagctgtatc ttctataatt ctagatagat tttgtggttt taatttaaat tctttgtata
     5701 ttggagacat tagagttccg acaaatacag aagtgaacat cattgatgaa ctaaatgcat
     5761 ttgcaaagta gcttactata aaagtagacc caaccaattt agttcttgat tttcctattt
     5821 tagttataag aggttgaagc agtacttcta agaatccagt tttttggaga ataccagcaa
     5881 taacgaaaac tacaaatact agtaaaacgg tttcagccat actcattatt ccaccacgat
     5941 ttaatagttt gtcggcatag acaaatccag tatctatagt aaatcctgat agcatgtaat
     6001 ttaaaacatc acctattttt tctccttctt gaaatactgc tataataagt cccatgatag
     6061 ctgaacttag tatagatata atgggtggct tttgtagcaa taaaagtaat aataaaaata
     6121 ttattggaat cattgctact aaaccaatat gaaagtttga atttaataca tccttgattt
     6181 gatttatttg aacatagtct atagtattat ttgaatactt aaatccaatt actgtataca
     6241 gaactataca tattacgtat gcaggtcctg tggtataaaa catatgcttc atatgtgtaa
     6301 ttacatctgt cttacaaact gctgcagcta agtttgtaga atctgataga ggtgacatct
     6361 tatctccaaa gaaagcacca gaaatgactg caccagctgt taaaccaaga ggaataccca
     6421 tactagtacc aatactcatc attgctatac cagaagttcc aagagtcccc caagatgtac
     6481 cagtagcaac agaagttaat gaacatacga ttaatgttgt aagcagaaaa tatttagggg
     6541 ttattattgt tagtccacta tatactactg ttggtattgt accagcagct atccaagctg
     6601 ctattagtac accaacagaa agtataatta agtttgattg aaaggcatct ttgccaactt
     6661 caaatccaaa agcttctatt tcatcattag tataaccaag tttcatggct gcaggaacta
     6721 ctataagcca tgaaagtaaa aacattgtag ttatagaagc attaaataca actataccaa
     6781 ctgatgttag agctattatt gcaagcatta ctaacaagga atatctaaat gaaggtattt
     6841 ttctttctgt attcattttc atagtgcaaa ttctccttaa atatttttag ttaattgcat
     6901 tttttattct tataagagct tcttcaagca tacttcttgg acaagctaag tttattcttt
     6961 gatacccact tcctccaatg ccaaagctgt ttccttgatt tagagcaact ttaccttttt
     7021 gtacaagtat actttctagt tcctcatcac ttaatccaag tgcactaaag tctacccata
     7081 ataaataagt accttctggt tttctaactt taagttttgg catattttca tttatatatt
     7141 ttatagcaaa atctatatta gattctaaat attctaaaaa tgattctagc caagattcac
     7201 cattattata tgaagcttca gttgcaacta agctaaagca attatttctc tttatatcaa
     7261 ttctggtaaa ggcatcatct aacaatttat agtatttttc atcaggtagt attacatatg
     7321 atgattgaag accagctata ttaaaagttt tagttggagc catacaagta attgtatttt
     7381 tttcaaattc tttagaaata gatgccatag gaatatgctt atgttttttt aatataatgt
     7441 cactatgtat ttcatcagat atgattttta cattatgctt aagacaaata tctcctagtt
     7501 tttttaattc atcttttgtc catactctac caactggatt atgaggatta caaagtatga
     7561 ataattttac atctttaatc ttgttttcaa tgtcttcata atccattatg tagtttccat
     7621 tttctaattt ttgtaatggg cttataatta gttctctatt gttatttttt acaacactat
     7681 taaaagggct gtaaactggt tcttgtatca ttattttatc atttgctttt gtgagttcat
     7741 ttattaaaag acttatagct gggataacac ctggactata aattaaccac tcacttttta
     7801 ttttccagtt atgtctccta tctagccagt ttactataga ttcattgtaa gaatctggtc
     7861 ttgttgtgta gccataaatc tcttgttcta gtctattttt taaagaatca attatacaag
     7921 gtgcagcctt aaaatccatg tcagccaccc acataggaag taaatcatta gttccatact
     7981 ttttttccat ttctgaccat ttagatgaaa aattattgct tctatcaaca atctcattaa
     8041 aattataatt cattgtaaat acctccagta attttaattt tatgttaata atattaagca
     8101 aacaacgtgc caatattgat aataaaaaat atttattcac aagttaatat ttaatattaa
     8161 gtaatttcag agcatagata agataaaaat attaattata cttaaaactt tgtggaaaag
     8221 gttatttcta ataaattttt aaatatatga aaatgtaatt aaataggtta aataggttta
     8281 actgtttaac ttgttagacc tatttatatt ttttagaaac tctactccct ttgatgttat
     8341 aattgaacct ccacgaccaa caagtatgtc tacaagccca aagtcttgta gttctaaaag
     8401 catatttcta atctgttgtt ctgtcaaaaa tctattttcc tctaaagcta ttttacataa
     8461 gctttttcta cctattcttt cttttaaatc ataagccttt tttaaattat ccaatataaa
     8521 tatgtattca tctagtgacc ttttaaaatt atagtcgtat ttcatgatgt ttttgtcttt
     8581 gccaatgttg aattgatact tttttatatt attacttact ttattacaaa cggttctact
     8641 ataatttgaa tctatagtat ctaatatata ttcaggtaaa tcacatattt ctataatatc
     8701 tttttccaag taacagaaat attctcctaa gtttcttaat tctcttacat ttccttccca
     8761 tctatacatt ttaaagattt cttttacttc ctcacttaga ataaaattac atggaatatc
     8821 atattttaat gaaccaaaaa tttcaaatat atcagcaggt ctttccctaa gtggatgtat
     8881 tttaagggga agaacattca acctaaaata taaatctctt ctaaacatat ttttagatac
     8941 aagttctttt aaatttctat tagtagctgc aattattctt acatcaatgt caatgacatt
     9001 attagaacca atacgtcgta cttgtttttc ttgtatgact ctaagtaatt tagattgaga
     9061 atttaaatcc atttctccaa tttcgtctaa aaatatagtt ccattgttgg ctaattcaaa
     9121 taaaccaatt ttcccacctt ttttagcacc agtaaaagcc ccttcatcat agccaaataa
     9181 ttcactttgc agtagatttt cttgaaaagt actacaattt actgcgacaa aaggaccatc
     9241 ttttctccta gaagcactat gaatggattg agcaaataat tctttaccag taccactttc
     9301 accaattata aggatagatg aatttgattg agccatttta ttagcaattt tttttgtatt
     9361 aatagtttgt atactagaac ctaatatatc atcaaatgtg tatttactga tattaccaga
     9421 gtttactagt tgtgctctta atttagcttg ctttttttct aattgagaaa actttgtcat
     9481 ctttagtata aatccatcaa atacatttaa ttgtatatgt ttaatctcta aatttatatc
     9541 aatatgattt attttaatca atttctcaaa aaatggagtt ttattggtca atatgctttg
     9601 aaaattgatg tcttttatgc aatcaccaac aaatgagtca atcatttcat ttgcatttat
     9661 agacaaaatt tttctagcca tatgattata gaattgaatt attccatcgt tatttgtgca
     9721 tataattcca tcatcaatta tagatagtaa caaatcaaat tgtctttcaa acttatttgc
     9781 atctaacaat aactcacctg tactataact tgtaggaatt attttttcta cgtatttttt
     9841 tattaggtca tcttttatta gatgttttaa tttggttttt gtagcaattt caacaataca
     9901 acttaaatct attattctgt attttaaatc tataacttca caatttttaa acttaggcaa
     9961 gattttttcg ccaggagtta ggattacagc atctgtggga gtaaactcaa tctctggata
    10021 gcagggcagg atatttatat tgtctattcc taatctatgt attaaagaaa ttgtttctat
    10081 tgccatatct ttacttaagt tatatacata aataatttta tctttttcta atttggatat
    10141 catatcaata ctgttctttt caaatgtcaa cttaggaaca atgatttgag catcatagtg
    10201 agataatttt actatttcat catattttaa ataagcagat aggagtataa gtttttcttt
    10261 aaaaaacttt aaatttcctt cttcaaatga gtataaattt atctttagat tatcacctaa
    10321 gaattgtctt atttgatttt cataaatttc tccagcgtct ttttttaaac ttacaatagc
    10381 taattccatt tgtttcattt taacctctca tacaaatttg acttatttta gattacataa
    10441 taattaaatt agattaaata atattattta attattatta taaatttatt atagactaca
    10501 cattgaagta aaagaagtag ctaaaattat tccttttatt tttgtttttt gtatctattt
    10561 ggtataatag tattagagcg tataataatt taggcaaaat tttttaagga agactagagg
    10621 agaatataaa atgaaaaaat gtctttactg taatagggat ataaagtcac caaataataa
    10681 attagctata aaatatgatg acaatatgaa tatatatcca tgtcgtgcag aatgtaagga
    10741 aaaaattgaa gaatatatag caaaaaaacc tacatataag tttataaata tattagaggt
    10801 tcttttaggt gtgggcggaa tattctgctt tttgagtaaa tggactattg cagcccaagt
    10861 tgtacttgga attgatgcac tggttatatt tttattccca ttggcaggat ttaagatgaa
    10921 tggaaagtta agtatggaac aaactaagaa tcaaagtagg tctattgcaa tatcaatttt
    10981 ggcatttata gtaataataa ctgtattata ttttaaacaa attggtaagt agagcataat
    11041 tgtataagac atcattagag tatgaggtga gtttatggaa gaaagaaaat tagcaccaaa
    11101 caatgagagg gaagctctca taaaaagatt aaaaagaata gaaggtcaag ttaagggtat
    11161 acaaaagatg gtggaacaag aacgttattg tgttgatata ttggttcaaa tatctgctat
    11221 aaggtctgct ataaatagag taggcacaat tatcttagag aatcatataa aaggatgtgt
    11281 agcagaaagc attaaacatg atgatgctga taaaactgaa gagatgataa aagagcttat
    11341 ggatactata aataaattta caaaataagg aggcggttag ttatgaatca acaaggattt
    11401 attatggaaa ttgttgatga tagaactgca aagttaaaaa tgcaaagaca ttcagcttgt
    11461 gctgcttgtg gaaaatgtgc tactacaaat tcagcagagt caaaagaaat attagtagaa
    11521 gtagacaaca caattggcgc aaaagttggt gaccatgtag aagtgagtat ggataatatg
    11581 aatgtattaa aagcagctgt aatggcttat ataatacctc ttatagcact tcttgtagga
    11641 acaataggga cttattatat attgggggca attggaatga caactggagt tgaagccata
    11701 agtggattag ttggaatttg tcttacaata atctgttatt tatacttaaa gaagaatgac
    11761 aaaaaattta gagacagtag agaatttata ccagtaataa caagaatatt aattgacctt
    11821 taagataatt ataattaaaa aatagcaatg tgcttcttaa ataaatactc aatacgtatt
    11881 tgtagagttt aagtacattg ctatttttat atttttattt tgattttctg taaggaataa
    11941 agttttttct gattttcaaa agattcaatg ttcttacaaa agaagttaca actaatacac
    12001 caagtgctat tgcagcactt agaaatagcg catctgtacc ttttgacata gccaaagaat
    12061 aatttttctg aatcaaagac aacattgtat tatatagccc aattcctggt attagaggaa
    12121 gaatacctgg aattacgaac acaatagctg gttgctttag ttttcttgct aaagtctcac
    12181 tgcacgctga gaccaaagca gcagcgataa aacctgcaaa aactgcattt gtagtaaaat
    12241 taaataaata aacatataca atccaaccaa ttccgccagt tactccagct ggaagaagta
    12301 gatatagtgg agcattaaag aaaacagcaa atccagcagt tgcaacacct gaaaataaaa
    12361 aatgcaaaat catagacata tcagtcatta gaaaggacct cctattctat accatgtatc
    12421 aaggacaaaa ccaactccag aagcaattgc aataataatc atagcagcat caaaagcacg
    12481 agaaattcct gatattaaat tccctgatat taaatctcta atagctttta taaaagcaac
    12541 tcctgggagt agtggcatta tagcgccgac tataagcatt ttagggtcat ctaaaatgcc
    12601 tatttcaact agtagaactc ctgaaagtgc aattaaaaaa gaagatacta aacaggaaaa
    12661 tgcagatatt gcacttatct tcatcgtttt atcataagta attgcagcaa agattgctat
    12721 tataaacgta agtataaaat ttaatacata attccctcca agcaaacaag caaaacaagc
    12781 agatgcaatt cctgtacaag agtaaaataa ccataaagga taaggtttta catcttggat
    12841 ttcataaaga cgtgcaatgg cattatcaat atcatactct ttgtcactaa caaattctcg
    12901 agaaaaactg tttaataaag aaatcttatt taagtttata cctcttgatt gaatcgtttt
    12961 cataaacgaa aagccatcaa atttctcatc tgaaataatt attactgtag gagttgtaaa
    13021 aatatttaca tgataaaacc ctcttgacct gcatattcta aggacactgt cttcaaccct
    13081 agatgtttca gctccatttt ttatcatcag ttcccctaca aatagagcta gttttagaat
    13141 atttttctta tattcaagtt cctcattttg attcataaat acacctccaa attaaaaaac
    13201 gtaatattga aattatatca aatttttaag taatattcct taatatctat acaaatatat
    13261 tatctttttt ttgtttcaaa atagtcacat ttaaattata tccaattaaa agaattagac
    13321 aatattattt attatatttt aacttaaaaa taaagaaagt aactatgtta aatatggaaa
    13381 aatggcaaaa cacttgacta ttaattggta aaggactata ataataaatt atcatattga
    13441 taaaaacgaa aattatgaaa tgtctgtaaa aataaaattt aaataataac taggaggagt
    13501 aatatgtttg agaacttgaa aaaagcggac cctgagattt atgaaagtat gaaaagagag
    13561 ttaaaaagac aacaaagaaa tattgaatta atagcatctg aaaacatagt atcagttcca
    13621 gtaatggaaa ctatgggaag tcatcttaca aataagtatg cagaaggtta ctcaggcaaa
    13681 agatattatg gtggatgtga atacatagat gaagttgaaa ctcttgcaat agatagaatc
    13741 aaaaaaatat ttggagcaga acatgctaat gttcaacctc attctggagc aaatgcaaat
    13801 attggtgtat attttgctat gttaaaacca ggtgatgttg ttatgggaat gaatttatct
    13861 caaggtggac atttaactca tggtgctcca gttaatattt ctggtcaata ttataaattt
    13921 tatgaatatg gtatagataa agacagtgga cttatagatt ttgatgaagt tagaaagata
    13981 gcacatgaag taaaacctaa aatgatagtt gcaggagcaa gtgcatatcc tagagaaata
    14041 gattttaaaa agtttagaga gatagctgat gaggttggag cattgcttat ggtagatatg
    14101 gctcatatag caggtcttgt ggctgctggt cttcaccaaa atccatgtga agtggctgat
    14161 tttgttacaa caacaactca caaaacttta agaggacctc gtgggggtgt tattctttgt
    14221 aaagaaaaat atgctaaaga catagacaaa gcaatattcc ctggtattca aggtggacct
    14281 cttgaacata taattgcttc taaggcagtt tgctttaaag aagctttaag tgatgagttt
    14341 aaagaatatc aggtacaagt tgctaaaaat gcaaaagcct tagctgaaga acttattaaa
    14401 agagatttta aattgatttc tggaggtaca gataatcatt taatactatt agatttgact
    14461 aataaaaata taactggtaa agcagcagaa aagagattgg atgatgctta tataacagct
    14521 aataaaaata caattccatt tgacccaaat ggtgctcttg ttacaagtgg aataagactt
    14581 ggtactccag ctgtaactac tagaggtatg aaagaagaag atatggcaat aatcgctgag
    14641 gctatagacc tatgtttaac ttatgatgaa gaatctaagg ctagaacatt ggttgttggt
    14701 ttaactgaaa aatatccttt atatgaagaa tataatatta tggattaatt ataaagaaca
    14761 actaaataaa atacatttaa ataattacaa tgattgtgta tgtatttgaa tatatttaga
    14821 tataaaaaag gtgtctcaaa atgaactttt tagtttatga ggcacttttt tgtatggaat
    14881 cgaacatatt tgtattattt aaataataaa aaatatggtt atctctattt ttagatggtc
    14941 tctttgtatt ttttaattaa aactttaaga tttcttaaga attatactag gtttttctaa
    15001 agatttaaag tctatactta taagtatagt aatcatatga aaagaaaagc tttaacgagg
    15061 agggaatctt atgaaggtat taattcttac aggaaaattt ggtatgggtc attactcagc
    15121 atcaaattct ttaagtgaag atataaaagc aaaatttgat aattcagaaa taataattaa
    15181 agatatattt gagtacatta tgcctaacta ttcagataaa atgtacaaga ctttttctat
    15241 tcttgtaaat cggggaagta gtctttataa tttgttttat aaatgtgctg aaaatggaaa
    15301 gaaagatata aaatttactt tttcagatta ttttttaaat aaattagata cattacttca
    15361 tgaagtacaa ccaacagtag ttatttcaac ttttccattt tgttctcaat tagtatcaag
    15421 atataaggaa aaatataact caaatcttcc tttaataaca tgtataactg atataagtag
    15481 tcactcagaa tggattagta aaaatacaga ctgttattta gtagctagta aatctactaa
    15541 ggaagaactt gtttttaaag gaatagatga atctaagatt aaagttaatg gtattccagt
    15601 aaaaaaagaa tttaaaagaa tagaacatgt aaatcattca actaaaaaaa atattcttat
    15661 aatgggtgga gtattaggat tactgcctaa atcagaacaa ttttacaaag aattaaatag
    15721 tttagaagga gtaaaaacta ctgtaataac tggtaataat aaaaaaatgt actataaact
    15781 atatggtaga tatgaaaata tagaggtagt aggttacacc aatgaagtat ataaatatat
    15841 gaaagattct gaccttatta tttctaagcc aggtggaata acactatttg aaacaattta
    15901 ttcagaactt ccaatattgg catttaatcc atttttacaa caagagatag ataatgctag
    15961 ttttatatta aataatgaaa ttgggagaat acttggtaaa aataaaaaat actatgtaga
    16021 tgagataaaa gatttaattt atgatgatgc tactcttaaa gaaatgagca gtaacatgaa
    16081 agaattaaaa aaacaatttg ataataatac cttagagaat attttatttt cacttgatga
    16141 acaaggagcg tgtagagaat gtatgtagta ggtttaatta tcgttgttgc attgattttt
    16201 ttagtccatt caattatacc tacatactat aataaactat taaataaaga ggtattaaaa
    16261 aatatggctg gagaaaatga aattgcactt acatttgatg atggtccaga taagagatat
    16321 acagaaaaat tactagatgt gttaaaagaa aatgatatac aagctatgtt ttttgtagta
    16381 gcaaagaatg ctgaaaaaga gcctgaaatt ataaaaagaa tgttgagaga aaatcatata
    16441 gttggtttac attcattgga acacagaaat gcatggcttt atagttatag ttatgttaaa
    16501 aaagatttta ttgaaagtac aaatataatg aaaaatttag gtgtagatgt aaactactat
    16561 cgtccaccat ggggtcatac taatatattt tcaaattcat ttgttaaaaa atataatcta
    16621 aaaatgactt tgtgggatgt aatggcagaa gattgggaga aagatagtac agtagacatt
    16681 attataaaca agttaatgag tagaacaaaa gaaaattcta taatttgttt acatgatgca
    16741 ggtgaaaatt caggtggtgc agtaggtgca cctgagcgta ctattgaagc tttaaagata
    16801 gctattccaa aacttaaagc cagtggttta aagttcgtaa caccagaaag gatgtagtta
    16861 tgataagtaa aactgaaaag aaaaataaat gcctaggtag tgtagctttt ttagttttac
    16921 taatgggcat aacaggatat tttgttttta gagggcagtc agttgaatca ttgataaaat
    16981 cattaaaagg tgcaagccct atgtttatac taattggatt tgcaatgatg tttatatatg
    17041 ttgcctgtga aggtataaat atatacttag gtatgaaagc tttaaaccaa aagactactc
    17101 ttttaaaatg tatgggatat gcttttattg gattttattt tagttctatt acgccttcag
    17161 catcaggtgg tcaaccagca caagtttatt atatgaaaaa agacgatata aatatatcat
    17221 attcttcact tatactatta gtcattgttg ttattcacca agtagttata ctggcatata
    17281 gtggaataat gttcattatg gaacgtgagt ttatattaaa taatgtaagt ggtatgaaca
    17341 ttttactaat atatggtgta ataacaaatg ttgctcttgt cattggagta attgcaatta
    17401 tattttcaaa aaaacttgta aacaatttta taatatcaat aacaaattta ttaggtaagt
    17461 taagaataat aaaagatgtt gaaagttcaa gaaaagttat aatatctcaa attgaagagt
    17521 atgttaaagg tgctcaatat ataaagcaga atccaaaact tgtagtccaa attttagtca
    17581 taactattgt acaaataaca tctatgtttt tagttccttt ctttgtatac aaagcttttc
    17641 atctatcaac atatactgta tttgagatat tagcgataca atccttgctt aatattgctg
    17701 tttcttcatt accattgcca ggggctgttg gagcttctga aaatagtttt atgacattat
    17761 ttaagatatt tttccctggt cacttattag ttccggcaat gttacttagt aggggaataa
    17821 gtttttatgc atttgtagct ataagtggat tgatatgtat agtagtacat gtaaaatctt
    17881 ctgaaaaagt aaaagcagtg aaaaaagttg cttatgtaag ataatattat atagattatt
    17941 tttattaata tgtcaatttg tgtgtatatt ttaattaagc tttaaacatg tttacaggag
    18001 tagaaactat taaaacgtgt gacattgcta agcttttgct aataaagtat aatattttat
    18061 atagtttgaa atatttaaga aatatagcaa aaaaaggagc ttggtgatga ataaagagat
    18121 tccaaaaaaa attttagtag ctgatgataa tcctgagatt agggaaatcg tagaaatact
    18181 tttaactggt gaaggttatg aagttattat ggcaacgaat ggagaagaag ctgtaaactt
    18241 agtagatagt acgattgatt tgattatttt ggatgttgta atgcctgtaa aaacaggatt
    18301 tgttgcatgt aatgaaatta gagaaaaaac gacagcacca atattatttt taactgcaaa
    18361 aactcaagac tctgataaag ttattggatt ttcagcagga ggagatgact atttatcaaa
    18421 gcctttttca tattctgaac taatttcaag ggttaagtcg ctacttagac gttattatat
    18481 ataccaggga aaggaaaagg aaagagagcc aaatatatta aaaacaaaag agttatgtgt
    18541 caatttagat actcaagatg ttacactaaa tggagaacct gtgtttttga caactataga
    18601 atatagtata ttaattctta tgttgaaaaa tagaaaaaaa gttttttcgt ctgaaaattt
    18661 atatgaaact atttgggatg agcagtattt ttatacagca aataatacaa taatggttca
    18721 tattagaaat ttaagaaaaa agttagaaaa agaccaaaaa aatccacaat atataaaaac
    18781 cgcatgggga aaggggtatt atattgatta acaaaattaa gccatctaag ttgggagtta
    18841 tactattgat atatgtagta atctcagctg cagtggcttt cattttaaat ataccatttt
    18901 actctgctgt tgaatctata gtaaaagatg tatcagaagg gaaagtatat tttaaaagag
    18961 aagtaaatca acttatggat aattttcaaa cttatgtgaa taacaatggt ataagagcag
    19021 atgatggaga tgatatagca aaatggaata gtgataattg gtttgtttct atgaagatat
    19081 ataaaggaaa taaggttttt tatgatacat tatactatcc taatgaagta cctgataaga
    19141 gtgaggaaaa tgagttttat agcagtccca tggctagaaa ctatgagata caattttctg
    19201 atattaaagc taatatagtt gtagtagctt tatttagatt tagatttcaa aattttatag
    19261 aagcaatttc tattacattt atggcactta catttattat aacattttta ctgttgttta
    19321 gtagaaagat aaagtaccta aggaaaatag aaatgggtat aaagatagta gagagtggca
    19381 gtttaaagta tagaatccca gtaaaaggta atgatgaatt atcatcactt gcatctagta
    19441 ttaatgagat gagtcataca ttggagaatc aacttataaa agaaagggag atgaagaata
    19501 aaaataaaga tatagtaact tcgatatcac atgatgttag aactccatta acgtcagtaa
    19561 tttgttattt agatttgatt aaagaccata aatacaatag tcctgagcaa ttggaacaat
    19621 acataaaaaa tgtaaggtct aaagcatatc aaataaaaga tttaacagat gaccttttta
    19681 cgcattttgt taattctgga gaggaagttt caaaagggta tgaaaaaata aatgctaatg
    19741 aatttatttt tcaattactt ttagatagtt catatacatt ggaggagaaa ggatttcata
    19801 taacaataga aaatgaactt tataaccaat atcatattta tgtagatgtt acacaattta
    19861 gaaggatatt tgataattta tgttctaata taataaaata tgctaggaaa gataagccta
    19921 tagtattttt aattaaagta gaggattcta aattactaat tatacaaaaa aatagagtaa
    19981 gtaatttttc taagaatgta gaaagttttg gaattggtat aaagaattgt gagaaaatag
    20041 ttgcatatca taatggaagc ataaatgtaa attcagatga taaagaattt gctattgaaa
    20101 tatctattcc agcaaaagaa tcataaaaat ttaaattata tattgtgtaa ttgctattat
    20161 atattgtgta attgcttatg taaaattcta tgaagcagga agcatgtgac tttagttatg
    20221 ggatgttcaa tattgtgtac ctctaggcaa gtaacaagtt tacttacaag accatataat
    20281 attattatct taaaaaaagg ggacataata atatggtttt gacaggagca cattatattt
    20341 acattctatt tatggtaatt atattaatta ctatgattat gaaaaaggat acaatagtac
    20401 catgtatatt aggtgttttt tttatgggat tgttctttga aaaaaacatc tttggtgcta
    20461 taacagcagt gtttaactct tttattatat ctttaaatga gttgggtcca attatcttga
    20521 ttatagctat tatggttgca ttatctaaag ccctagaagc caataatgct atacagtata
    20581 tggtgagacc attttcgaga gttataaaaa attctaatac agcatttttt gtaactggat
    20641 ttgttatgtt agttctttct tggtttttct ggccaactcc agctgttgcc ttagttggag
    20701 ctgtattttt acctgttgct atgagagctg ggctaccagc aattggtgtt gcagtagcat
    20761 taaatttatt tggtcatggt cttgccttat ctacagactt tgttattcaa ggagctccaa
    20821 gtataacagc tggtgcagca ggtgttgcag tatcatatgt tataaatgat ggtatgattt
    20881 tgttttgggt gatgggaatc gtaaccatat ctatggcatt ttatacatta aaaagagata
    20941 ttaacaaagg aatgtttaga gaagaactaa aaggttttga aagtgaagaa gtagaagcgt
    21001 tcaatggaaa atctaaaata gcaactattt tagtagcttt gggatttctt gctgatataa
    21061 ttgctatgta tgtatttgac ctaaaaggag gagatgcatc agctcttttg ggtggaacag
    21121 cagtattttt aataataatt ataaatgtta taaattttgg caaagatagt cttgaaaatg
    21181 tatgtgagaa tataattgat ggttttgtgt ttgggataaa aatatttgga gcaattatac
    21241 caatagcagc atttttctat atgggtgaag ttgcaccatt gacaggtgta tttggaaaag
    21301 tattagcacc aggttcacaa ggtttattat cagatatagg catagctctt tctcaaacaa
    21361 taccacttaa taaatttgcc gtatcaggta tagaaactgt agtgggagca ataactgggc
    21421 ttgatggttc tggattttct gggatttcat tagttggttc acttgcctct gtatttggaa
    21481 ctgctataaa tgcaagtgtt ggtgcattag ctgcattagg acaaatcagt ggtatatggg
    21541 taggtggagg ctgtctagtt ccttggggtc ttatttcagc agcggcaatt tgtggggtta
    21601 gtccaataga attggcaaag cgaaactttg tcccagttat aaccggattg gttgtaacaa
    21661 ccatagtagc aatgtttatt atttaataaa tcgaataaat ctttaattgt ataaattaaa
    21721 aatagatgtg tagctataat ttattataat tgttttacta attctaagcc atacatctat
    21781 ttttattgat atttttatta gtattcttta ataaagtaat atttttcatt atattataca
    21841 taacatctaa atataacatc taaaatactg ttatctatat ttaatatctt gccaaatttt
    21901 ttgatgtttt attgccctgt cccaccaaca tatataagct atcacaagtt tttacctata
    21961 ctatcaccaa acaaaaattc taattttaat ttattatttt ataacctata ttatattaac
    22021 tatattagtt tgtagacttt agatgtaatt ataaatatta atactccaag agttcaaatt
    22081 catatcctag ttttcttgca tttgatataa aatcatttaa aatttctgta ctagttttgg
    22141 aaactgcatg tagtaacaag atagaaccat catgtagatt atccattatt ttttgcgttg
    22201 cttcttcatg tgatggttgt ttatcagtat cccagtctct gtatgcaaaa ctccaaaata
    22261 cagtcttgta tcctaaattt ttagtcattg ccaagctttt ttctgagtat tttcccatag
    22321 gaggtctaaa aaatttaacc atatctttcc cagttacatc tttatataac ttttcaacat
    22381 catataattc gtcattaaag ttttttagat tgcttgtttt tgttggcata gatggatggg
    22441 ttttactatg atttccaact atatgtccct cagaaaccat tctttttact aaatctttat
    22501 tttcttttat atatggagct gtaacaaaaa aaactgcttt aacctggttt tgttttagag
    22561 tgtcgagtat ttttgtagtg tatccatttt cataaccttc atcaaatgta agatatagtg
    22621 ttttttgacc atcttttgta ggtccattgt atagggcatc atagtcagaa aatttaaatt
    22681 caatgtccgt atttacttct ggtgttttat gcttattgtt tggtatataa aaccaatcaa
    22741 gagtagtgtt atctaaagaa gatacatcta atttattatt atcattatta gtagtggctt
    22801 ttgatggttt atctgttccg tctgaaacta ttacattgga agtatcttta ccactatcta
    22861 ttttttcctt atcttcttgt aactgtgttt ccttattttg attttcgttt tgattgtttt
    22921 gtgaatttga acatccaaca ataaaaaaaa tagaaataga aagtagtgtt aaataatgca
    22981 atacttttct aaacattttg aagctccttt ctaaattaat aataattgca tataataata
    23041 attatatgat taattacaat tgcatatagt attattataa ttggaaatta ctattaataa
    23101 tagttatatc tttaaaaata aagtcacaat agtatgacaa agggtgaata atcaataaat
    23161 ttaaaaaaaa atgaaaaaaa tagttgacca tattatgaga agatgttatt ataataaatg
    23221 tgctcaacaa gaagtaacaa aataacaagc acaaagaagt taattgaat
//
LOCUS       DAMJVI010000057        22508 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.059, whole genome shotgun sequence.
ACCESSION   DAMJVI010000057 DAMJVI010000000
VERSION     DAMJVI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22508
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.059"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            18..1298
                     /locus_tag="PC545_002266"
     CDS             18..1298
                     /locus_tag="PC545_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDF2457033.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPNEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKTLNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIESDICSA
                     TAIRTSLKENTDLFHLKNVIPEKTYNILNEKIDNGFLPMFDDFFFDILKTIILRDMDT
                     LKEYFEVNEGIENRIYKDIFTSEFLHELQLAIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREILKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKATNIYNSIYYKNNKDLVKGPMDFYTHPIYVK"
     gene            complement(1561..2952)
                     /locus_tag="PC545_002267"
     CDS             complement(1561..2952)
                     /locus_tag="PC545_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDF2457034.1"
                     /translation="MYKLIDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEIINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLIPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(2963..4183)
                     /locus_tag="PC545_002268"
     CDS             complement(2963..4183)
                     /locus_tag="PC545_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDF2457035.1"
                     /translation="MSKIVVNQDKITDLKRILEICPFGAIEEKSGIVEISAGCKMCKL
                     CVKSGPKGAFEFIESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCSNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFIDKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSAPEKIENTTGKITLCKVEKKELKSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMIYKLADKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDNNQKYMASAAK"
     gene            complement(4197..4985)
                     /locus_tag="PC545_002269"
     CDS             complement(4197..4985)
                     /locus_tag="PC545_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2457036.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKEDKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEVKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLNTKDREIPVYSLDDFEDKNENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSFALVNKLEELKLV"
     gene            complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC545_002270"
     CDS             complement(5002..6276)
                     /gene="larA"
                     /locus_tag="PC545_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HDF2457037.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRNVNPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHISTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSVLPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSKVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMISACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTIPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEMANSIVGKESKVVVIPDGVSVIVK"
     gene            complement(6394..7107)
                     /locus_tag="PC545_002271"
     CDS             complement(6394..7107)
                     /locus_tag="PC545_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2457038.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLEDILEL
                     RIILEIEIAKLASKRITSSEVIELKHIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEN"
     gene            7232..8491
                     /locus_tag="PC545_002272"
     CDS             7232..8491
                     /locus_tag="PC545_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HDF2457039.1"
                     /translation="MKKNKIIAFFAPSIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNGQISKYEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNENLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSSPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGVLAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTIIQIVVIIYLISAVFIAKK
                     HMKTLSTNGKLMRYKNTFFTKKFSRKK"
     gene            8759..9325
                     /locus_tag="PC545_002273"
     CDS             8759..9325
                     /locus_tag="PC545_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2457040.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKYLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(9526..10731)
                     /locus_tag="PC545_002274"
     CDS             complement(9526..10731)
                     /locus_tag="PC545_002274"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HDF2457041.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNTSKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNNVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDNNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRSSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILECLDKYVDSYDIIDDEELAIFVSAKSEHEIDRVLEIVKRNNYSE
                     SAKKLLKID"
     regulatory      complement(10834..10940)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(11032..11400)
                     /locus_tag="PC545_002275"
     CDS             complement(11032..11400)
                     /locus_tag="PC545_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HDF2457042.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDTKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     gene            complement(11452..12729)
                     /gene="obgE"
                     /locus_tag="PC545_002276"
     CDS             complement(11452..12729)
                     /gene="obgE"
                     /locus_tag="PC545_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDF2457043.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELEGRGYTVFKMSAATRQGIDEVIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            complement(12840..13130)
                     /gene="rpmA"
                     /locus_tag="PC545_002277"
     CDS             complement(12840..13130)
                     /gene="rpmA"
                     /locus_tag="PC545_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDF2457044.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            complement(13144..13461)
                     /locus_tag="PC545_002278"
     CDS             complement(13144..13461)
                     /locus_tag="PC545_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDF2457045.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECIVKDTHVKDAQLLLNHFQLAVKEIKREYPKNIKI
                     LKK"
     gene            complement(13476..13787)
                     /gene="rplU"
                     /locus_tag="PC545_002279"
     CDS             complement(13476..13787)
                     /gene="rplU"
                     /locus_tag="PC545_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDF2457046.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            complement(13888..15309)
                     /locus_tag="PC545_002280"
     CDS             complement(13888..15309)
                     /locus_tag="PC545_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HDF2457047.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNIYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSTIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCTHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYKTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     KEILHILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDSIEKEIMRISEHTVYKNIMVEFNDFIFEQIKEYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            complement(15327..16028)
                     /locus_tag="PC545_002281"
     CDS             complement(15327..16028)
                     /locus_tag="PC545_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HDF2457048.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPDGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKTNLNTNILIPKILEMFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            complement(16030..17874)
                     /locus_tag="PC545_002282"
     CDS             complement(16030..17874)
                     /locus_tag="PC545_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HDF2457049.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            18193..19386
                     /locus_tag="PC545_002283"
     CDS             18193..19386
                     /locus_tag="PC545_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HDF2457050.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDAEVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDSMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            complement(19598..20680)
                     /locus_tag="PC545_002284"
     CDS             complement(19598..20680)
                     /locus_tag="PC545_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HDF2457051.1"
                     /translation="MKKIRKLATKLLIAMMVLTSCMSVTVYGMTAKDVTAKTPKSYVA
                     GTNSIYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKIRVAYNHIKNNTTYVNWNA
                     AEGANTAYTLVTKKGACSGMCRSLKALCDAMGIECYYVHSTTNDHQWNLIRFEDGRLY
                     HVDIDANKSAGADIIYKSLTHPLPFDKKAYPAVGEVVKTQNPTPTVKKPTPAKPSAPV
                     GPGNHTMIADQQGAILDEVYGNNLRPKIEMYEGVPCSLINSSINTIKFTSKNSNLDPS
                     KHTVTVIGFMNTPYGMVALAPDGVPDEYPFEYDKPFSLDFTGKNLSAAELSKKCKENN
                     VIIKVEIGETASNSGRGMVAYARYVN"
     gene            complement(21244..22035)
                     /locus_tag="PC545_002285"
     CDS             complement(21244..22035)
                     /locus_tag="PC545_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457052.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLDRHKSIVMGFKKVLGYISIIAFLIS
                     GFMWYYNNSLQHNMYNLGKELDGEIKQLAERHDMTKIGEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYEREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFAFGLLCIIAYFILRFKISLIKNNN"
     gene            complement(22039..22368)
                     /locus_tag="PC545_002286"
     CDS             complement(22039..22368)
                     /locus_tag="PC545_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457053.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
BASE COUNT         7245 a         3989 c         2513 g         8761 t
ORIGIN      
        1 gataaaagga gtattttatg aacatacttg gattaatagt tgaatataac ccatttcata
       61 atggacacat atatcattta tctaaatcta tagaaaaaac aaatgcaaca catactgtag
      121 caataatgag tggaaatttt ctacaaagag gcgaaccagc tttatttgat aaatacacta
      181 gagcaaaaat tgctgtcgaa aatggtgtag acttagttat agaattacca actttatttg
      241 cttgtcaaag tgctgaaatt ttttctcatg gtgctataac aattttaaat tcactaaact
      301 gcataaattc tatatgtttt ggtagtgaag aaggaaatac agaaattctt tataatatag
      361 ctgaaatttt agcaaatgaa ccaaatgaat ttaagttact tttaaaaaaa tatttagatg
      421 atggtctgtt atttccaaca gctagagcta atgcactttt tgattatata catcaagaca
      481 aaaaaaataa ttttatacaa aattattcaa aggaacaatt atcaaaaaca ttaaattcat
      541 caaacaacat actgggtata gaatacataa aaaacctcat ccatttaaac agtagtataa
      601 aaccatttac tatctccagg gtccaatcag agtataattc cgaagaaatt gagagtgata
      661 tttgttctgc tactgctata agaacttcac ttaaagaaaa tacagacctt tttcatttaa
      721 aaaatgttat tccagaaaaa acttataaca tcctaaatga aaaaatagat aatggttttt
      781 tacctatgtt tgatgacttc ttctttgata ttttaaaaac aattatactt agagatatgg
      841 acactttaaa agagtatttt gaagtaaatg aaggcataga aaacagaatc tataaagata
      901 tctttacatc tgaatttcta catgaactac aattagcaat taaatccaag cgatacactt
      961 taaccaaaat caaacgaact ctaaacaata tattgttagg tattaaaaaa agcgacatgc
     1021 aacttataaa aaacatagat actatacctt atataagaat acttgctttt aatgataaag
     1081 gtagagaaat tctaaaagag ataaaaaata aatctgaaat aaaaataata aacaagtttt
     1141 caaatatatc attttcatta gatgatacga tttttaaaac actcataagt tatgatataa
     1201 aagctacaaa tatatataat tcaatatatt ataaaaacaa taaagattta gtaaaaggcc
     1261 ctatggattt ttatactcat ccaatatatg taaagtagtt ttttaaaata actatattta
     1321 taaaaatcat tttattaact ctttattata taagatacaa tagtcttact ttatttatgt
     1381 acacatgctt ttaggatgac aacatcaact taaatattat gttactttct aaatttaagt
     1441 aaatattgcc atcctaaaaa ttttacaatt tagcttcaga tttaaaagta aagtttaaaa
     1501 tattatattt aaatctaatt taatattttt tgttttgtaa atattattaa attaatattt
     1561 ctaatatatt ttccctggat ttaaaatatt tttagggtca aaagctaact ttatattttt
     1621 aattaacatc atataagcat ctgaatttga ttcatgtaag tactcttttt tagcatatcc
     1681 tatgccatgt tcaccagaaa cttgaccact aagttctcta gattttttat acatatagtc
     1741 aaaagtttct tttaatctta ttttccatgt attatcatcc ataccatctt ttaaaatata
     1801 tatgtgtaga tttccatctc ctgcatgacc aaaacttttt attcttattt ttaatttgtc
     1861 ttgaagctcg tgagtgtatc ttataaactc tgctatctta tctcttggaa caacgacatc
     1921 acattcatcc atttgtgtcg tagatgcctt tatagcttct aaaaatgcac ctctggctga
     1981 ccatatagaa tcgtttcttt cttgtgtatc tgatataaat acatctaatg caccattttc
     2041 taaacataaa ttagctactt tttcatattc tttttctata tcttcagtgc tatttccatc
     2101 aaatgtaagc aaaagataag catctgatgt attatctgga aactttttac ctaaaaattc
     2161 ttctgctgcc aatatcacat ctctttccat aaattctata gccgttggta ttgattttga
     2221 ttttattatt ttaggaacag tttctattgc cattgataaa tctggaaatg gtataagcaa
     2281 acttatactt ttctttggta aaggaagcaa ttttaatata gctttagtaa ctattcctaa
     2341 agtaccttct gagcccacta ataaatcctt tatactatat ccagaactat ttttaaccac
     2401 ctttccacct acatttataa tttctccatt tggtagtaca acttcaagac ctcttacata
     2461 atcccttgta acgccatatt ttactgctct cataccacct gcattagtgt ttatattacc
     2521 tgcaattgta gcactttttt cacctgggtc aggtgggtaa aataaatctc tatcttgaac
     2581 atattgacct attgtcatca ataaaacacc tggttctact gtcaatgtta aattttcata
     2641 atcaacttca agtattttat tcattttaca tagattaatc attattccac catttatggc
     2701 aacagcagca cctacaagac cagtaccttg acctcttggt gtgactggta tattattttt
     2761 atatgcgtat ttcattattt tagatacttg ttcagtttca agtacattta ctaaaacttc
     2821 agggtacttc tcaatccctc caagttcatc atgtgaaaaa tcttcattta tatcacttcc
     2881 aactaataca ttttcttctc cacaagtatc tattaaaaaa tctatatctt ttttatcgat
     2941 taatttatac atttctatcc ccctatttag ctgctgatgc catatatttt tgattattat
     3001 ctttgttatt ttctattttt tcaatcaatt taggaataat atcataaata tcacctatta
     3061 aagctaaatg tgctacatca agtataggcg ctttatcatc tttatttatt gctactatat
     3121 agtcagcacc ctgcatccct gcaacaaatt gaacagctcc agatacacca caagttatta
     3181 tcaatttagg ttttacagtt ctaccactaa gacctatttg ttttttagca tctatccaac
     3241 ctgcttctat tacaggtctt gttcctgcca cttgagcgtt taacttatca gctaatttat
     3301 atatcatctc catatcttct tgttttttta ttgctctact tgccacaact atcacttcag
     3361 cttcttccag accaacttct ttattttttt cttttacact cagtacctta attttagatt
     3421 ttagttcttt tttttctact ttacataaag ttatctttcc tgtagtattc tctatttttt
     3481 ctggtgctga aaatatttta tatcttacag tggcaaattg tggtctatta ttaggtgtgt
     3541 ttatatgagc cattatattt ccaccaaatg ctggtcttat ttggtctaaa tcagtatttg
     3601 attgaatatc aagtattgta caatctgctg taagacctgt tctaaatcta gcggcaagtc
     3661 ttggagctaa tgacctacct aatgtagttc caccaactaa tactatcgtt ggttttattt
     3721 tatctataaa gttttctatt gcttttgaat atggttctat cctaaaatct ttaaattcat
     3781 cttcatcata tacaaatact tcatccaccc cataagaaat caaattactg cacttatctt
     3841 ttatgtcttt tcctacaaat acacaataca caggttgttt tatttttgat gccatctcac
     3901 gagctttacc tataagctcg tacgttacag gatgtatatt tccattatga tgttcaacat
     3961 atactgcaat tcccctccat tcatctttat taatttgtac tttactactc tctataaatt
     4021 caaatgcgcc ttttggtcca cttttgacac acaacttgca catcttacag ccagcactta
     4081 tttctactat acctgacttt tcttctatag ccccaaaagg acatatttct aaaattctct
     4141 ttaagtcagt tattttatct tgatttacaa ctatctttga catttattac tcccccttac
     4201 accaacttta attcctctaa tttatttaca agtgcaaaac ttagttcatc tgcatttcct
     4261 cttacaatat ttttatctgt atttggcttt ggaggaaata ttcttacaac ttgagtagga
     4321 gaaccattaa gtccatagtt attttcattt ttatcttcaa agtcatctaa gctatataca
     4381 ggtatttctc tatctttagt attaagtttt aatttaaatg aaggtagtct tggttgaaaa
     4441 atgtcttttt ctacagttat taaacatgga tattcaatct cacatacttg taaatcattt
     4501 ggcatatcta tttcaacaac tatactttca tctttaactt caattaattt tgtaacgtta
     4561 gtaacatgag gtatatctaa aaattcagct acttcaggtc ccacctgtgc tgtatcacca
     4621 tcagtagttt gttttccaca aattattagg tctacctttc ccatcttctt aataccttgt
     4681 gatatggtat aggaagtagc caatacatct gctcctccaa atctcctatc acttattaat
     4741 gctccttcat cagctcccat cataaaactt tcttcaatta ccttctttgc ctgaggagga
     4801 cccatgctaa gtacttttag attcgctttc ttatcttcct ttattctaag agctgtttct
     4861 aaagcatata ggtcataggg attcattttt gaatctactc catctctttt tagtactcct
     4921 gtagtttcat caacttctac tgaagtcgtt ccaggaactt gtttaataca tgtcaaaatt
     4981 tccataaagt acactccttt tttatttaac tataactgat actccatcag gtataactac
     5041 tactttgctc tccttaccaa ctatactatt cgccatttct aaagcttctt taaagttaaa
     5101 tgcatgtttc atatgcattt tttttatcat ttcaggatta cacttatctg ttaccattat
     5161 cacagtatat ttagataata ttctcgctag tatctgaaat tcccattggt ctggaatcgt
     5221 atctactcta tcgacacttc taattttatc taaaagttgt tttggagaat ctgcatttgc
     5281 tacattttca taaaaagatt gacctccatg accatcatta catgctgaaa tcattattat
     5341 tactccaccg tctttacaag tagcttcagc tgctgtcatt cctttaacag attgataaat
     5401 attttggtct agtggatatc caccatttgt agttacaaca atatcagatt ttatagaatt
     5461 tacttttgat aactctgtta caaatctaca tcctttagta tgtgcttttt cgacatcacc
     5521 agcaaaggaa gcaattattt ttttatcttt atctattaca acattcaata tgaatgctaa
     5581 ttttgcaact ttagctgcat ataccatatc ctcatgaata ggattattat tcaattttcc
     5641 agttcttgaa ttatcactat ctataaagtc actacagtga ttgtacatta tagtctttgc
     5701 agatgcgatt cctggaagta cgctttttct gccacctgaa aaacctgcaa agaaatgagg
     5761 ttctataaaa ccttctgcta tcaaaagttc tgcctcatat gctagtttgt ttatatataa
     5821 atctccacct gaaggcaata tccctacttt acacatagat ttatcatcag tagatatatg
     5881 cataactata tcttcattat ctactatttc ttctcccatt ttataaatta gctcttccct
     5941 tgtacttggc ctatgaaatc cagttgccac tataatcttc acatcaattt ctggattaac
     6001 attcctaatc cttcttaata taattggcat agttattcta cttggtacag gtcttgtatg
     6061 gtcacttgta attatgacta cattcttttt ccccttaacc agttcttcca gagaattaga
     6121 gcctatagga ttattcatag attcttcaac tatctcttct tgagtcttat gttctatata
     6181 attatccgat tttgactcca aaacaccgag aaaacgttca tcaggtattt ctaaaatcat
     6241 tccttgtttt gaatacggta cttttaactt taccattttt aatctcccat tataataaac
     6301 tataaaattg gtatgtggtc ataccagttt tacatttata taatagtacc attatctacc
     6361 atttgcaaga ataatttatt ttataacaaa cttttaattt tcattttttc tataattttt
     6421 acttataaaa tccatatgtt cctttgcttt ctcacatgca agttctacat cattttctac
     6481 aactgcattg tatatagctt catgctgttt aagcaatata ttttcattaa aaggttctgc
     6541 gattattttt tctcttgcat cttctataaa tccatcaaat aattttgatg ctgtcataaa
     6601 aagactttga attaaataat ttttacttaa tgttgctagt ttactgtgaa atttttggtc
     6661 taataatacc agtactcgat tattatcttt tttattagtt tctaccctca tttcatctat
     6721 tatatgtttc aattctataa cttcagaaga agttattctc tttgaagcaa gttttgcaat
     6781 ctcaatttct aggattattc taagctctaa tatatcttca agtgtaccat tattaagttt
     6841 aaaaatcata gataaaggtt ctattaaagt tttttctata tttgtacata taaaatttcc
     6901 ttcaccctgt ctgctttcta tcactcccat agtctctaat accctaatag cttcccttat
     6961 ggaagttcta ctcacattca tttgttcaga cagttctctt tctgatagta atttatcccc
     7021 cttttttaac tctccattta gtattttata ttgtatttgc tcaattactt gttcataaac
     7081 ttttttacta gctatactat taaacatttt ttcctccaat atgataatcg ttgttgtaat
     7141 atatatctat tatatatctt aagccattac attcatatat ttttattact tttaatataa
     7201 tatcttataa ctcaaattta caggtgatat tatgaaaaaa aataaaatta ttgctttttt
     7261 tgctccatct atcatagttg gagttctagt tttagggatt atcctatacc caaatgaagc
     7321 tataaaatct gcaagagaag gcttttctat atggtctaat gttttagttc catcactact
     7381 accatttata attggagcaa acttaattgt tgatttaaaa atagtggata ttatagggtt
     7441 tatcatcaat cccataacta gatttgtatt taatgtttca ggaaagagcg ctttagcttt
     7501 tgctatatct acagtatctg gttatccagt aggagtaagt ttagcttctg aatttaggag
     7561 taatggacaa atttctaagt atgaagcaca aagacttgtg tctttttgtt ctacatcagg
     7621 acctttattc ataataggtt cggttggtac aggtatgttc caaaattcaa acttaggtta
     7681 tttaatgatt ctctgccatt atctaggaac tatattggtt ggtcttttct ttagaaatta
     7741 tggtaatgaa aaccttccaa agacaaaatt ggatttaaaa acaaatataa aaaatgtaat
     7801 caacattaga aattcaagtg ggaatggttt ttttgtttta tttggaaatg ctgtatttaa
     7861 tggagtaaac acattactta cagttggtgg atttgtaatt gttttttcag tagtatttaa
     7921 aatactatct ctttttaatg taatctcatt aatagcatca gttatatatt tgcctctttc
     7981 atttttaggt gttagtaaag aactctgtca agcatttgta agtggtctat ttgaaattac
     8041 tataggatgc aataaagtat ctagtataac aagttctcca gaaattttaa gagcatcttt
     8101 agctagtttt ttgataggat ttagtggatt atccattcta gctcaatgtt gtacatttct
     8161 atcaaaaacg gacatagatt taaaattata catactaagt aaatttgcgc atggagtttt
     8221 agctgctatc tttatattta tactttatcc aattgcaaac tctgctgtgt tggtttctag
     8281 ctttgctgat acatacaacg ttatatataa taatttaatc tggttttatt accttagcta
     8341 ttatgataca attattcaaa ttgtagtaat aatttatcta atctcagccg tatttattgc
     8401 aaaaaaacat atgaaaactt tatctacaaa tgggaaacta atgcgatata aaaatacttt
     8461 tttcactaag aaattttcac gaaaaaaata aaactaataa aacttaacta aatacttcca
     8521 tttttttatt atatttgtca taattttaat aaccttttta attagtttaa cagcatagtt
     8581 atttcatcac aatttaataa ttttattaga aatatttttc aaaaaattct ccttaatcac
     8641 atatatcaca cttatataag tttactggga aatttttcca ttatataata tttcatttat
     8701 attatataat aaataccgac ctaaacttac caaatcaaac taagaggagt ggttgaaaat
     8761 ggcaacaaga cctatagaaa tagacgaata taaaaagata atggaactat tacatactgg
     8821 ctttacttat agtgaaaatg gcgttgaaaa aagatttaga aaaaatccta aagttgcatt
     8881 ggcactaatg ttagaagcta atttaggtct aagaatatca gatattttaa gattaaagat
     8941 aggtaatttt aaaggtaata tgttggaaat aaatgaaaaa aagacgggca aattacagta
     9001 tagaccagtt aataaaaata taattgagtc cataaatgaa catgcaagaa agtataatct
     9061 taaatcaaat gactacttag taaatataaa gacaaaggct attcaaaaac agttgaggat
     9121 aatttgtaaa tacttaaatc tgtataatat aagtacacat agctttagaa agctctatgc
     9181 tactactcaa tttgaaaaga gtaacaataa tttagagttg gttaaagaac ttttaaatca
     9241 cagctctgta gccactacac agcgatatat aagggtaact cagcaagcta ttaatgaagc
     9301 tagtgaaaat ttctttattg gttaattata acgtatacat ttttctatta tcttattaat
     9361 tcaccagata ttaaaaaaga gtgtctcaaa atgaactttt tagttcatga gacactcttt
     9421 ttttataaaa tcaaatatat ttttattatt tcaacactgg aaaaaaggct tatctctcta
     9481 ttttgagaca ccctattaaa ttttcataat tcttgcataa acatattaat ctatcttcaa
     9541 taatttttta gctgattcac tgtaattatt tctttttaca atttccaaaa ctctatcaat
     9601 ctcatgttct gactttgcac ttacaaaaat cgccaattct tcatcatcta taatatcata
     9661 actatctaca tatttatcta aacattctaa aattccatct ttattattat acccaccaaa
     9721 tctaactact gcactacact catcttctat aagtccactt aaatccaatt tcccttttga
     9781 aaaagacttt atatttgaac ttctattatc tattatatct ataatatctg cataaacagc
     9841 acttcctgtt gcacctcttc cagctccctt acctacaaaa gaaagttcac ctactgaatc
     9901 acctactaaa ataactacgt tgaactcatt atctatcttt gaaagcatat tattattgtc
     9961 cactaatgct ggtctaacaa atccacttac ttctcctgat tcatacttac ttctagcaac
    10021 caattttatc ttacaattta atttttttgc atattctata tctttttcat ctattgaagt
    10081 tattccttct aataataaat ctttccaata tactattttt ccataagata aagtagacaa
    10141 tatagataac tttctagcac tatcatatcc taaaacatct gcatctggat taacttctgc
    10201 aaatcccaac tcttgtgcct gttttaaagc gacatcatac ggtaaatttt catcatacat
    10261 ctttgataat ataaaattac aagtcccatt taaaatagcg tttatgctct ttatattgtt
    10321 gccttccagt gattctatca atggttttaa aactggaata ccacctgcca cagatgcttc
    10381 aaacttgatg ctaacattat ttaagttagc taattctata aggtcactac ctttttcagc
    10441 cagtaaatct ttattagctg tcactacatg tattttttta tttaacgctc ttgttacata
    10501 atcaaaagct ggatttaatc ctccaattac ttcaactagt atatcaatat ccttgtcaaa
    10561 tatatcttct atatcagtag ttatcttatc aaagtacttc ttatacttgt gtttttcttt
    10621 atttttaact aaaattccaa caacttctat atctcttttt gaagtattat tttctattaa
    10681 atcaattaat ccacttccta ctgttccata ccctaatatt gctatcttca ctgtaacata
    10741 cccccatcat tttgattata ttaaattatt tatatatata aatataccaa taaaaaaagc
    10801 ttgagtataa actcaagcca tatgacgggt ttatactcat ctttcagcat aaatgctgca
    10861 ggatttagca ccaatgtata attaaatata ctggttgccg ggcttcacag ggccagtccc
    10921 tctgccactc ttgataagat tgctaattta aatttatgtt taatagtata atacaatttc
    10981 ttccaattgt cactattttt ttaacttatt tccctaaaag gatataaact attatctaac
    11041 agctctttta gttggtttac ccttttttgt aacattatta tttttagtct ttgctttcgc
    11101 ttgttcatgt cctggaaaaa gtattttagg ctcttcaata ttcctcttgt aaatagtaaa
    11161 tttaaaacca atagcctgca caaattcagc tcttaaagct tcacatactg cattagcagt
    11221 ttctttagta tcaagacctg cattttctaa tatagtaact tttactaatt ctctagttct
    11281 taacatacca tctaattgct ctaagaaact ctctgtaaca ccttctttac caatttgtgt
    11341 agtaggcttt aaagtatttg ccaaagatct taaatacgcc ctttgttttc cttttaacat
    11401 attctcacct ctataattct atacttaaat tatctataat ctatatttaa attaattgta
    11461 aaactcaaat tctaattcat aaattttaac cacatctcca tcttctattc ccatttctct
    11521 aagtctatca aaaacacctt gactctccat agctttttgg aaatattgaa gggattccat
    11581 atcatcaaaa tttacagaat acattattct tctaagtgct ttacctgtaa ctacatacac
    11641 accatcttca atatctatac ttaactcttc ttcagtacca atatcaagtt ctggtctata
    11701 catttcttct tctgaaacta gctccacatc ttctacttct tttagcattt tagaaacata
    11761 ggctattact tcatctatgc cttgacgagt tgctgctgac attttaaata ctgtatatcc
    11821 tctgccttct aattcactct taaacttctc aaacttactc tcatcttcta atatatcaaa
    11881 tttatttgca actacaactt gtggtctctt tgaaagcttt tcattgtaca atttaagctc
    11941 atcatttatc ttgtcaaaat cttcaatagg gtctcttccc tctaatccag atatatctac
    12001 aatatgtatc aatacctttg ttctctcaac atgtcttaag aactcatgac ctaatcctat
    12061 gccttcagaa gcaccctcta taattccagg tatatctgca agtacaaaac tatctccaaa
    12121 cttagtttga acaacaccta gatttggtgt aagtgttgta aaatgatagt ttgcaatttt
    12181 gggtttagct ttagttacaa ctgatagaaa tgttgattta cctacatttg gaaatcctaa
    12241 aagaccaaca tctgctatca ttttaagttc aagagttatc catctttctt ctccatctgt
    12301 accagatttt gcaaaagctg gagcttgtct tactgcattt gcaaagtgtt ggttaccttt
    12361 accacctata ccaccttttg caactatagc tgtatctcct tcttttttta aatctgctaa
    12421 tacaagacct gtagcctcat ctcttattac tgtacctgct ggtactttta atactaaatt
    12481 ttctccattt ttaccagctc tttttccttt gcttccgtcg ccaccatttt gagcttgata
    12541 ttttttctgg tacttaaagt ccattagagt tctaagcccc aaatcaactt caaatatgat
    12601 gcttgctcca cgaccaccat ctccaccgtc tggaccacca gctggaacgt atttttctct
    12661 tctaaatgct accgaaccat ttccaccatt tccagcctta acaaatattc ttgccttatc
    12721 tataaacaaa ttcttcacct acttaattaa aaacttttaa ttagcattac caaaaatacc
    12781 attataaatt catcagtatt ataagaaaaa ggagtgcctc aacactcctt ttatgtatat
    12841 tactcagcta ttgctactgg atatatacta actttttttc tcttcttgtc ttttctttcg
    12901 aatttaacag taccatcaac taatgcaaat aaagtatcat cactaccttt tccaacatta
    12961 gttcctggat gtattttagt tcctctttgt ctaactatta tacttccagc agttactaat
    13021 tgaccatcga atctcttaac acctaatctt ttagatatag aatctctacc atttttagaa
    13081 gaacctaccc cttttttaga tgctaataat tgtaaattca tgtttaacat atggagtcac
    13141 cccctacttt tttaaaattt ttatattttt tgggtattcc cgtttaattt ctttaaccgc
    13201 taattgaaag tgatttagta ataattgtgc gtcttttaca tgtgtatctt tgactataca
    13261 ctctatatga ccttcttctc ctactacttt ttcaaattca atcttctgta atttgtctaa
    13321 agaatttata actgcaatag cattagatgt aactgcagca catactatat catagcctat
    13381 ttcagcaaaa tcagcatgtc cctttaaaca aaaaccttgc aacaagaaat catcattata
    13441 ataatattta acttttatca taacttaacc aataactaag cgtttatttt ttctacaact
    13501 atcttagtgt atgattgacg atgtccttgt tttcttctgt aatctttttt tgctttatac
    13561 ttgaaaacta taactttctt agctttacct tgttcaacaa ctttagcttg aacagatgct
    13621 ccttctacta ctggagaacc taattttaat tctccatctt tagagcaagc taatacttcg
    13681 tttaaagtaa caacatcacc tgcgttagct tctaattttt caacgaataa tacatcacct
    13741 tcagaaactt tatattgctt tccaccagtc tttactatag cgtacattaa atacacctcc
    13801 tcggcctcaa actcgccagt acaaggtact tcatttgaag atttaaacct ttactgtgcg
    13861 gcattacaat tattaattat aaaatattta cattttattg tcaactattt tatcgaaaat
    13921 aacattggtt ttattgtgct ttaatgtact atttgcgttt aaagaaatct ttatattata
    13981 tttttcacca attttatcaa ttatatccat ataatattct tttatttgtt caaaaataaa
    14041 atcattaaat tctaccatta tgtttttgta aacagtatgt tcacttattc tcataatttc
    14101 tttttcaata ctatctagta tataatgtac agattttatt gtttgttcac catcacaaca
    14161 tggacaagac attaaataat acttatcaat ggattctttt tctctacgcc tagcaacttc
    14221 tactaaacca agttttgtca tacctaatac ttcagctttt cttttatctt tattaaattc
    14281 ttcttctagg atgtgtaaaa tctccttttg atgcttcttt ttatgcatat caataaaatc
    14341 gattattatt atcccagcta tgtctctaat tctaagctgt cttacaattt cttttgcagc
    14401 ttcaagatta gttttataaa cggtttcatc aagtttacca gttccaataa acttacccgt
    14461 atttacatct ataaccgtaa gagcttctgt tttttcaatt attaaataac ctccactctt
    14521 taaccatact tttttattta ataatttttc tattttagat tctattctgt ataaatcaaa
    14581 tacatctcta ttttcctcaa gacacagttt atcaacataa gtcttatcta tacctcttaa
    14641 aatagactta agttcagagt acttatcata actattggta attatagatt caatatcatc
    14701 attcacatta tcttttacat atttagttgc aaaatctaaa gacttatata ataatttcgg
    14761 acctatacca agtttatatt cttttaaaat gttttcatac tttaatttca actcttctat
    14821 atctttcttt atttcatcat gagtgcaacc ttgagcctca gtccttatta ttaatcctag
    14881 attttcctta tttactgcct tagtaatttt ttttaatcta aatcgttctt tttcatcagt
    14941 aattttattt gatattgtag ttctatcatt ggaaggtata tagacaatat accttccaga
    15001 aatactaatc tcagtgttta attttgcgcc tttagttcct atttcttctt tatttatctg
    15061 aaccaaaata tcctgaccag attttatagt acttcccttt tttaattgta aataagcaag
    15121 tttcttaaat ccaatatcta caaaacaagc ttctattcct ggtataactt tttttacgat
    15181 tccacgatat atattggata cagtcttctt atttaaattg tcttcaacaa aaagttctgt
    15241 taatctttca tcttcaagta ctgctgtttt ctgggaccct attagggact caataactat
    15301 ctttttcaca acttcacctc tcgagcttac ataggagtca ctaattcccc atcttctaat
    15361 acatataaat ctcttctcaa tatatcaaca tctaatgggt caatatcaag ttcaaacatc
    15421 tctaatatct ttggtataag tatattagta tttaaattag tttttgagcc tgtagctata
    15481 gtagcagtaa gagtaatatg ttcatcttct aaatttaaaa catcaaaggt tcttatcata
    15541 ggtcttatat ctacttcaac catcttacct tttttattct tttttgttat gattatttct
    15601 ttattattca taaagtcaat tacttttctt tttacaaact ctttcgtcat aggtcttttc
    15661 aaatctatat taaacaaata ttctccatag tctatcacag aagaaagaga aggtgtttgt
    15721 ctatcaattt cttttgcttt gataaaatct atcccatctg gaagttgaat tgatacttta
    15781 tttaaaaatt cctctactga taaatcatct tcttctattt caatatctac atactcacct
    15841 tgactttctg tccctaaagc caaagcattg ccataactca ttttagggtg aggattaaat
    15901 ccttgagaat gtgataagtt tatttcagct cttctaaacg ctctttgtaa caatctctga
    15961 aggtcaagat gtgatatata tatcatgtcg ccttcctttt taaattttac tcttattatc
    16021 ttactcatat tagcaaagcc cccttcctat atcatgtata tttattccac aaccattgca
    16081 gttatgtcta caatcagaag tgattttttc ttttttagca ttttcgtctt ctctaattaa
    16141 gaatttttta ctaataccta catctatatg gtcccaagga aatacctctt catagtttct
    16201 atttctatgt gcataaaaat ctatagaaag attattcttt tccatagctt ctttccatat
    16261 atctaaatta aaatgttctg cccatccatc aaatttagca cctagtctaa atgcatcata
    16321 tataactttt cctattcttc tatcgcctct agctactact gcttccatca aactagtctt
    16381 tgaatcatga taattgtatt ttatattgtt atttcttaat ttatttacta aatgtctctg
    16441 cttattaata acttcttctg tagtatcttg accatgccat tggaaaggtg taaatggctt
    16501 tggtacaaat gtagaagtac tcactgtaac actaaaactt ctctttagct ttcctccatt
    16561 tacttttcta taaatatcaa taactttata agctaattta gctataccat ctaaatcttc
    16621 ataagtctca gttggtaaac caatcataaa atacaactta acactattcc accccatctc
    16681 aaaagctctt tctgttgcat tttccaaatc ttcttctgta acacctttat ttataacatc
    16741 tctaagtctt tgagttcctg cttctggagc aaaagtaagt ccagattttc ttacttgttg
    16801 tattttatca gcaatttcca ttgaaaaatt atctaatcta agggatggaa gcgaaatccc
    16861 aatattatta gatgcatatt catctaccaa ataatcagtc aactctgata acttactata
    16921 atcactcgta ctaagtgatg ataatgatat ttcatcatat ccagtattct taactaattt
    16981 atctaatatt tctttaagtc ttttaacact cttttctctt attggtctgt atatcatacc
    17041 tgcttgacag aatctacatc ctctagtaca tcctctaaat aattctagta ctattctatc
    17101 atgaacagta tctatataag gtactattag tttttctgga taatctacag tctcaacatc
    17161 ttttattatc cttttagtag gatttttagt aatcccttct ctattaggag ttacactttg
    17221 aactgtacca tcttcattgt acttgacatc ataaaaactt ggtatgtata ctccttctat
    17281 gctagatatt ttatataaaa aatcttctct agtcgtttta tttttcttcc attctttata
    17341 ctcatttact acttctaaat taacctcttc accttcacct aatattacta catctacaaa
    17401 atctgctaat ggttcggaat tataagcaca aggaccacca accattataa atgggtcttc
    17461 caaagttctt tcttctttta atattggtat attggctaaa tctaatatat ttaagatatt
    17521 agtgtatgaa agttcatatt gaagtgtaaa agtgacaaaa tcaaagtttg ttataggctc
    17581 tcttgactct aatgcaaata atggtatact tttttctctc attatatttt ccatatcaac
    17641 tgcaggagaa tacactctct cacaaaatac atcttcatcc ttatttatta catcatataa
    17701 tatatgactc cctaagtgac tcataccaac ttcatataaa tctggaaagc aatgtgcata
    17761 tcttatcaat tcactattac tagtatcttt atgaattgaa tttatttcat ttccaagata
    17821 tctagcaggc ttttctactt tttttaagat ttttttcaag tctactttat tcatttatta
    17881 aaattcctcc aaacaagctc tttattttta ttcttattta taagtaatat tatacacgat
    17941 aaaatatttt ctatcaaaaa tataactaaa atattgaatt taaaatacat attattcaca
    18001 aaatagaata gctaaaaata tttaagaata ttttctatta aatttttatt gaaatacaat
    18061 ttttttacaa agtatgatat attataaaca taaaactatt gttttacatg acttatcatg
    18121 acatcttttg tttcattcac ctcaaaatgg ggtatgttac ttatataatg ataaatttta
    18181 ggagggttaa aaatgagtaa agtttttgaa gtaaaaaaag atatctactt tactggagta
    18241 gtagatgaag gtttaaaagt attcgacata ataatggaaa ctgagtttgg gacaacttac
    18301 aactcttatt taatcaagga cgaaaaaacg gttttatttg acactgtaaa ggcaaatttt
    18361 aaagatgagt ttttaagtaa tttatcagag gttactgata tagctaaaat tgactatgtt
    18421 gtaattcatc acacagaacc agaccatgca ggtagtttaa aatacttatt agatataaat
    18481 cctaatatag aagtttattg tactaaagct gctaaactat acttagatgg tcaaataaat
    18541 agacctttta actgtcatgt aataaaagat ggtgaaattt taaatatcgg taaaagaaat
    18601 cttagattta taactgctcc attcttacat tgggttgata caatgtttac ttacattgag
    18661 gaggacaaga ctcttttaac ttgtgatgca tttggttgtc actttgcaag tgttgatgca
    18721 gaggtagtga atagtgaaga ttatctaaaa tcagctaaac actactatga ttgtatagtt
    18781 aagccatttg ctaaacatgt tttaagtgct gtagataagg tggtaggact aaacatagaa
    18841 tttgatacta tattgacttc tcatggtcca atgcttacta aagactcaat ggctgctgtt
    18901 aaaagatatg ttgagtggtc tacagaagct gttaatacaa ctaatcaaaa tcaagtttct
    18961 atattctact tatcagctta tagtaatact ttagagatgg ctaaaaagat aaaagaagga
    19021 cttgataaag aaggtgcaaa ggctgaatta tatgatttag aagatatgac attaacagaa
    19081 atgcacgata cattagtagt gtcaaaagta atattactag gttctccaac tataaacaaa
    19141 actatggtta aacctatgtg ggatttattc tctgttatag acccaatggc taaccaaggc
    19201 aagatagcag gagtatttgg ttcatttggt tggagtggtg aaggtataac tatggctgag
    19261 actttactta aatctatgtc attcaaaatg cctgtagaat ctcttaagaa aaaattcttc
    19321 cctagtgaag agacattaaa agagtgtatg gcatttggtg cagaatttgc aaaattagtt
    19381 aaatagccat taatcaaaca taatattatt taaaataaat gattttatat ttaaaaataa
    19441 attaaaatgg agttgtctca aaattaattt ttctaatcta attttaagtt caactccttt
    19501 tttattttct aaaatgactg atataatgat aaatgcaccc tttgtgcgag ggtgcattat
    19561 ttataatatg ttctatttgt acactagtat atttgtatta attcacatat ctagcgtatg
    19621 ctaccatccc tcttccagaa ttagatgccg tttcaccaat ttccacttta ataattacat
    19681 tattttcttt acactttttt gataattctg ctgcagaaag gttcttacca gtaaaatcta
    19741 aagaaaatgg cttatcatat tcaaaaggat actcatcagg tacaccatct ggagctaatg
    19801 caaccatacc atatggtgta ttcataaacc ctattacagt aactgtatgc tttgatgggt
    19861 ctaaatttga atttttgctt gtaaacttaa ttgtatttat tgatgagttg attaaactac
    19921 acggaactcc ctcatacatt tcaatttttg gacgtagatt atttccataa acttcatcaa
    19981 gtattgcccc ttgttgatct gctatcatag tgtgattccc aggtccaact ggcgcagaag
    20041 gcttagctgg cgtaggtttc ttaacagttg gcgttggatt ctgtgtcttt actacttcgc
    20101 caacagctgg atatgctttc ttgtcaaatg gtaatgggtg agtaagactc ttgtatatga
    20161 tgtctgctcc tgctgatttg ttggcatcaa tatctacatg atacaaacgt ccatcctcaa
    20221 aacgaataag attccactga tggtcatttg ttgtactatg tacataataa cattcaattc
    20281 ccatagcatc acataaagct ttcaagctcc tacacattcc agaacaagca ccttttttag
    20341 taaccaatgt ataagcagta ttggctccct ctgctgcgtt ccagttaaca tatgttgtgt
    20401 tgtttttaat gtgattataa gcaaccctaa tcttagcatc tgtggtcatg ttctttgtaa
    20461 ttttcttgtt cataaagtct gctgttgctt gtgctaccga attaagttga gcttgtgata
    20521 actttggacc atatatactg ttggttccag ctacataact tttcggtgtc tttgcagtaa
    20581 catctttagc tgtcatacca taaactgtta cactcataca tgaggttaaa accatcattg
    20641 caatcaatag tttagtagct aatttcctga tttttttcat attagcattc tcctttataa
    20701 tatattttac tttcaattcc ctaccattaa ttaagttcat tttagattta aaatatggat
    20761 tatcaacatg tatgcatagc aattcacgtg gtcatagaat atttagattt cttggaaaat
    20821 ctgttctata tttcctatat agttcgtata caatccaact cacaaacctg catttttgat
    20881 atcatcacaa taaaatgata ataattaata tattcaaaaa tgttggtctg taacaggtat
    20941 gtcaattttc caattattgg taatggcgag agtgaggaga attgtcaata atacatttaa
    21001 aattaaaatt gtatacactt catacattct cccctaacaa cattatacag attataattt
    21061 gtatattcaa tacatttttt ggaaagtata attatatcaa aatatgtgat tatgtataga
    21121 tacaatccac tatggagaaa tataaaagaa aagagtataa caaacaataa aaaataagat
    21181 gattcaaatt ttattttgaa acatcttatt tactctacaa attaagatta tttggtgaat
    21241 tatttaatta ttatttttta ttaaagaaat tttaaatctt aatataaaat acgctattat
    21301 acataataat ccaaatgcaa atgctactgg aattaacatc tccgattcaa aataaaagaa
    21361 atattctaag aaaatatctg gatttactat attcccattc ttgtctaaaa aatcttttcc
    21421 attatatcct aaatgctgta taaaattact tatgttatta taatctgctt ccctctcata
    21481 taccatatta gcattcaatc cagaagaact taaattagtg tttccatctt tcatatctat
    21541 tacatatagt ctaagagctt tgactttact atacttatct ttatcaagta ttttttcaag
    21601 ttctaactta tattcaccaa tctttgtcat atcatgtctt tctgccaatt gctttatttc
    21661 tccatcaagt tcttttccta aattatacat attatgttgt aaactattat tgtaatacca
    21721 cataaatcca gaaattaaaa atgcaattat acttatgtaa cctaatactt ttttaaaccc
    21781 cattacaatt gatttatgcc tatccaatga taaagatagt tcttttatta tattacataa
    21841 ttcatactgc atttcatcac catcatcaaa tctttcaatt gcttttttac atgcttcttc
    21901 ttcatctaat ccttctaatt taaattcatt tgcagattct ataagatggc atctcatttc
    21961 ctgttttaat tctatagtac ttttattgtc acatttttta tatagtttat ctaaatattc
    22021 atctattagt ttcataaatt aatccacctc tcccaaaaat tgattcataa cctttttaat
    22081 aaatctccat tcttgtttct ttatttctaa aaaatcttta ccttcattag ttaaattata
    22141 atattttctt cttccacctg aactttgttc attgctataa taagatttaa ttaaattttt
    22201 agattccatt cttttaagtg caagatacat tgttccttct tttaattcaa actcacttct
    22261 ttctcttact tgctttgcaa tttcatatcc ataacaatct ttcttttcta atatagaaac
    22321 aattaatata tcgatataac cttttaacac ttctttattt aactgcatta tgtaccaccg
    22381 ccttttcacc tactactcta taatgtatag tacttatatt tactataata ttacatagta
    22441 ctctataatg caaggtttat attctaaaat agactcaaaa ataataaata gctgtatatt
    22501 aatattta
//
LOCUS       DAMJVI010000058        22389 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.060, whole genome shotgun sequence.
ACCESSION   DAMJVI010000058 DAMJVI010000000
VERSION     DAMJVI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.060"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(355..495)
                     /locus_tag="PC545_002287"
     CDS             complement(355..495)
                     /locus_tag="PC545_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457054.1"
                     /translation="MENLMMSIITGVIASYIYDKLKSHSERPTKSGWELNIKFQKSKS
                     SK"
     gene            complement(753..1913)
                     /locus_tag="PC545_002288"
     CDS             complement(753..1913)
                     /locus_tag="PC545_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp27"
                     /protein_id="HDF2457055.1"
                     /translation="MKLNKTLLAIGMSVSLLIASCPTSNALSSINSIKGTNKYETAGL
                     IADKQNYDIAILINSENGLSDGLSASGLSGAKNAPILLTKKDTIPSETLKRLEKVKEV
                     YLIGGKNSIGASVQKILSDKKISINRISGTDRIDTSHKVAKEIGSVSAVKKVILTNAF
                     KGEPDAMSVASIAVRDKSPIVLTDGNTTSFATDGLETYAIGGKVSISDKLINETKATR
                     IGGVDRYDTNKQIINKFYNGSKEFYIASGTDLVYSLVGSTIAQKTPIVLVGNGSNKSI
                     LKNASKITAIGNIDDVVIKQCLNITNNVGDSNTGIVKDTNSNNTNKPSNEPSSNNPGL
                     TDKSTVYVTPNGKSYHLTKNCSTLKRSKTINKVTLKQAKSQGKIDPCNVCVK"
     gene            complement(2084..2287)
                     /locus_tag="PC545_002289"
     CDS             complement(2084..2287)
                     /locus_tag="PC545_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HDF2457056.1"
                     /translation="MGFNFRKSINLGGGLKLNLGKKSVGISAGVKGARVSVNSKGRKS
                     ATLSIPGTGISYTKTSTKKRKPK"
     gene            complement(2374..3408)
                     /locus_tag="PC545_002290"
     CDS             complement(2374..3408)
                     /locus_tag="PC545_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HDF2457057.1"
                     /translation="MFKKKLIAVAMSITMISVGSFSYAHSGRTDSSGGHRDNKNKSGL
                     GSYHYHCGGYPAHLHNNGGCPYTGGGSSSGTATSVNNEEKQKRSVGEKGYNQGYEDGY
                     KGNYSSSNYSGDYSDTYESKYSEGYEKGKAKLEEEEKEAKETGYNLGLTGAKSNNTYE
                     KEVLKNAYDTGYSTGYNEYKTKKIEEYKAKGIEDSNKDKEKMTFEENIDSKFIDAYNN
                     AYDEIQEQLKNDYTTQGFESAIKGERFDTSTIGNVKYANWFKEGYDNGKVKLPKAKES
                     VYNQGYNEEDFSIPDEMKSIETKLKGVYDEGLEKREEEKSRNATYGVGAGTAAVVAGV
                     VYKKKKRKKI"
     gene            complement(3930..5291)
                     /gene="rlmD"
                     /locus_tag="PC545_002291"
     CDS             complement(3930..5291)
                     /gene="rlmD"
                     /locus_tag="PC545_002291"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDF2457058.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEETCKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIIVNIVTS
                     SQEDFDMYEFKDMLLGIDLKAELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKGYGYEIKMVKCMDMFPNTPHCETVVKL
                     IRE"
     gene            complement(5318..6040)
                     /locus_tag="PC545_002292"
     CDS             complement(5318..6040)
                     /locus_tag="PC545_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2457059.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYISATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEVKLIEER"
     gene            complement(6040..6369)
                     /locus_tag="PC545_002293"
     CDS             complement(6040..6369)
                     /locus_tag="PC545_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HDF2457060.1"
                     /translation="MPQIKIRGINENDICKISEKMINDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEVAWFDRGQEVQDIVARIITDSIRNNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            6699..7625
                     /gene="fba"
                     /locus_tag="PC545_002294"
     CDS             6699..7625
                     /gene="fba"
                     /locus_tag="PC545_002294"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HDF2457061.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(7760..9391)
                     /locus_tag="PC545_002295"
     CDS             complement(7760..9391)
                     /locus_tag="PC545_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457062.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     RNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNENNIVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLTGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNVIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(9874..10911)
                     /locus_tag="PC545_002296"
     CDS             complement(9874..10911)
                     /locus_tag="PC545_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDF2457063.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(10930..11715)
                     /locus_tag="PC545_002297"
     CDS             complement(10930..11715)
                     /locus_tag="PC545_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDF2457064.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEHDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKVGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(11748..12881)
                     /locus_tag="PC545_002298"
     CDS             complement(11748..12881)
                     /locus_tag="PC545_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HDF2457065.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAGGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(12929..14056)
                     /gene="hadC"
                     /locus_tag="PC545_002299"
     CDS             complement(12929..14056)
                     /gene="hadC"
                     /locus_tag="PC545_002299"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HDF2457066.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTELVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(14056..15282)
                     /gene="hadB"
                     /locus_tag="PC545_002300"
     CDS             complement(14056..15282)
                     /gene="hadB"
                     /locus_tag="PC545_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HDF2457067.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEIAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLVEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(15297..16097)
                     /gene="hadI"
                     /locus_tag="PC545_002301"
     CDS             complement(15297..16097)
                     /gene="hadI"
                     /locus_tag="PC545_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HDF2457068.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(16136..17335)
                     /gene="hadA"
                     /locus_tag="PC545_002302"
     CDS             complement(16136..17335)
                     /gene="hadA"
                     /locus_tag="PC545_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HDF2457069.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKAQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            18012..19010
                     /locus_tag="PC545_002303"
     CDS             18012..19010
                     /locus_tag="PC545_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HDF2457070.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENVVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLINTGDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            19372..19899
                     /locus_tag="PC545_002304"
     CDS             19372..19899
                     /locus_tag="PC545_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HDF2457071.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASVVAGIVGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            19926..20522
                     /locus_tag="PC545_002305"
     CDS             19926..20522
                     /locus_tag="PC545_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HDF2457072.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAKFIKEKSSIKIRINTNG
                     LSDLIHNKKTAILLKNVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPEEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(20587..22170)
                     /gene="asnB"
                     /locus_tag="PC545_002306"
     CDS             complement(20587..22170)
                     /gene="asnB"
                     /locus_tag="PC545_002306"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2457073.1"
                     /translation="MINLEEKYLTRKDTYMIKILEYNGHIRNWKVLCEELGIDSSLCR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYETEN
                     GKLLYGSSIRKIMEQPGFVKELNEDMLQIYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIGRYWKPEFHPDHSKSIEEWTDEIHTTLQKIMPEVKSENETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEERFDESKLAQQTANILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADAFFGGYNIYHKAECYGENLKTFYI
                     GNTNIMNEGEKRKILKKYNPDVLPIEVVKGIYEETEELDTLTNMSNVDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRIPSEYKVKGKENKIAFRTAASKTLPEEIA
                     FRNKLGFIVPIRIWMADDQYNKDIRDKFNSEIADKFFNIDEINEIFNDYISGNSDNWR
                     KIWMIYTFLVWYEIYFVKC"
BASE COUNT         6933 a         4067 c         2674 g         8715 t
ORIGIN      
        1 ttagtcatct tcttaccagt tttttatcca cttttctaat gaagttattt tttcatatgt
       61 taattcctct tgaaataatg atactaaaag actttttata aaattataac ttaaaagttt
      121 atgtattttt ttgatatgaa tttatagtac ttatcttctt taattgagat tgtgtatatt
      181 taactaaaca ttacaacaat aattctatga aatcaaatca attatttaca tattttttta
      241 gaacaataat aagtatatat tcacaaaatt ttgtgttaat atttttaatt tgtgatataa
      301 taaaagcaag gaaacatatt ttacttaact ttgagtgatg tttccttcaa caaattattt
      361 tgaagattta cttttctgga actttatatt aagttcccag ccactcttag ttggtcgctc
      421 agagtggctc tttaatttat cgtaaatgta actggctata acaccagtta taatactcat
      481 cattagattt tccatagttt tcacctcctc tcataagaaa gtgaagaaac ttccatggaa
      541 acaccactca tagctttttc aaatatttat tccttgccat attaattata tcataaatat
      601 tggaaacata aaattttaat acttatcgtc tttattatta ttttttataa ttaataattt
      661 tttttaataa tacataatat aaatttatat ttcttcaata aaaaagacaa tcttaattaa
      721 ttgtcttttt taatatatat ttggctaata cattatttta cacaaacatt acaagggtct
      781 attttacctt gtgacttagc ttgttttaat gttactttat ttattgtttt acttcttttt
      841 agtgtagaac aattttttgt tagatgatat gattttccat taggagttac atatactgta
      901 cttttatcag ttaatccagg attattagag ctaggctcat tacttggttt gttcgtatta
      961 tttgaattag tatcttttac aatgcctgta tttgaatccc caacattatt agttatattt
     1021 aaacattgtt ttataacaac atcatcaata tttccaatag cagttatttt acttgcattt
     1081 tttaatatac tcttattact tccatttcca actaacacta taggtgtttt ttgtgcaatt
     1141 gtagaaccaa ctaaagaata aactaaatca gtcccacttg ctatgtaaaa ttcttttgag
     1201 ccattataaa atttatttat tatttgttta tttgtatcgt atctatctac tccacctatt
     1261 cttgttgctt tagtttcatt tattaactta tcactaatac tgacttttcc accaattgca
     1321 taagtttcta atccatctgt agcaaaactt gtagtgtttc catcagttaa tactattgga
     1381 gatttatctc taactgctat agaagcaaca ctcatagcat ctggttcacc tttaaaagca
     1441 ttagttaata ttactttttt aacagcactt acacttccta tttcttttgc aactttatga
     1501 cttgtatcta ttctatcagt tccacttatt ctatttatgc ttattttttt atcacttaat
     1561 attttctgaa ctgatgctcc tatcgaattt tttcctccga ttagatatac ttcttttacc
     1621 ttttctaatc tttttaatgt ttctgaaggg attgtatctt tcttagttaa caatattggg
     1681 gcatttttag ctcctgacaa tccacttgct gacaatccat cagataatcc attttcagaa
     1741 ttaataagta ttgcaatatc ataattttgt ttatctgcta ttaaacctgc tgtttcatac
     1801 ttatttgtac cttttattga attaatagaa ctaagagcgt ttgatgttgg acaactagca
     1861 atcaataatg aaacagacat ccctattgcc aatagtgttt tgtttaattt cataatgaaa
     1921 tcctcctata aattttataa tattacttaa atattataat tctacataaa agaatatttt
     1981 cctttatgaa attagtttat atttcaaaat tttgtcactt tttaaatcta tatactaaaa
     2041 aatagactat ttgtaacagt ctatttctta gtatatcaaa tatttattta ggtttacgtt
     2101 tctttgtact agtttttgta tatgatattc cagttccagg tatactcaat gtagctgatt
     2161 ttcttccttt tgaattaact gacactctag cacctttaac accagcactt atacctacac
     2221 tcttcttacc taaatttaat ttgagtcctc ctcctagatt tatgcttttc ctaaagttaa
     2281 atcccataga tataaccttc ccttctatat tttaaatatt tatacttaaa attatatttg
     2341 ttatacactt gccatcattg gtatatctag catctatatc tttttacgtt ttttcttctt
     2401 atatactact ccagcaacta cagctgctgt tccagctcca accccataag tagcatttct
     2461 acttttctct tcttctcttt tttctaaacc ttcatcatat acacctttta acttagtttc
     2521 aatagacttc atttcatctg gtatactaaa atcttcttca ttgtaacctt gattatatac
     2581 actttctttt gctttaggta acttaacctt accattatca tatccttctt taaaccaatt
     2641 agcatattta acattaccta tagttgatgt gtcaaatctt tctcctttga tagcactctc
     2701 aaatccttga gtagtgtaat cattttttaa ttgttcttgt atttcgtcat aagcattatt
     2761 ataagcatct ataaatttag aatctatgtt ttcttcaaat gtcatttttt ctttatcttt
     2821 attgctatcc tctatccctt tagctttata ttcttctatc tttttagtct tatattcatt
     2881 gtatccagtt gaatatccgg tatcataagc attttttaaa acttcttttt catatgtatt
     2941 attagattta gcacctgtaa gccctagatt atatccagtc tctttagcct ccttttcttc
     3001 ctcttctaat ttagccttac ctttttcata tccttctgag tactttgatt catatgtatc
     3061 actataatct ccactataat ttgatgaact ataatttcct ttatacccat cttcataccc
     3121 ttgattatat cctttttctc caacagacct cttttgcttt tcttcattat taaccgaagt
     3181 tgctgttcca gaactgcttc ctcctccagt ataaggacaa ccaccattat tatgtaaatg
     3241 agctggataa ccaccacaat gatagtgata acttcctaat ccagatttat ttttattatc
     3301 acgatgacct cctgatgaat cagttcttcc tgaatgagca tacgaaaagc tccctacact
     3361 aatcatagtt atactcattg caacagctat taactttttc ttaaacataa tctatttccc
     3421 cctaaaatat tctatgattt tattatatca gtcaatatat tatgtttaaa ccgttaaaat
     3481 tcgacaatgt aaatatttga tgaattgtat ttgtaaataa atcttttagc atatcttgaa
     3541 tatctgtagt tgttttgaca tcatattctt caataagttt tttaatattt cgtttttgtt
     3601 aaacataaaa agcgtgcact tttctttgta tagtgatatt tgatatttta attatcacta
     3661 ctttagaaga atacacactt tattttaaaa accctgatac tatgttatta gaaaatggta
     3721 ctaatataaa agatatccag catatactcg gacatgctaa aacttcaaca actatggata
     3781 catatttaca tgtaactaac aacatgagaa accagacggt aaatattttt gaaatcattg
     3841 tagaataaat ttgccaccca aaaataatta tggtggcaaa taggtggcaa atctactttt
     3901 aagacacact ataaaagtct gaaaccctgt tattctctaa taagtttaac tacagtctca
     3961 caatgtggcg tatttgggaa catatccata cacttaacca tcttaatctc ataaccatac
     4021 cccttaaaat caaccaaatc aacaaccaaa gacttaggat tacaagaaat ataaacaatc
     4081 tccttagcac caaatccaca aatatcccta atagcatcct tatgaatgcc tggtctagga
     4141 gggtcaacaa taatcaaatc aggcttgtcc tttaaatcct taacaacctt agccacatcc
     4201 ccagcaataa actcacaatt agtaagtcca ttcaacttag cattctcatt agcagcaaca
     4261 acagcttcct caactatctc aataccatac actttcttag cagcaccagc cataacctgt
     4321 ccaatagacc cagtaccact atacaagtca aatacaactt tatcattgta atctccaata
     4381 aaatccctag caatactata aagaacctca gctcccttag tattggtttg gaaaaacgaa
     4441 aatggtgaaa tcttaaactt aagacctaaa atttcttctt gtatataatc tcttccatac
     4501 aatactctta actcatcaca ttggactgca tctgctaaac catcattaat agtatgaagt
     4561 acacctacta actcagcttt taaatcaatc cctaaaagca tatctttaaa ctcatacata
     4621 tcaaaatctt cttgagaaga agttactata ttaactataa tctcattagt gtgaatccct
     4681 tttctaacta caagatgtct caaatatcct ttgtgattca ttgttctata ataaggaagc
     4741 tttttttcat tgaaaaactc tacactagaa actaatatct ttgagaagtc ttcatcaacc
     4801 aacatacact cctcaacagt ttgtatatct atatgctttc cttttttatg aagacctaat
     4861 gtcaaaggtc cattttttac ttcatctcca aaagtatatt ccatcttatt tctataatat
     4921 ttgttttctg gacttccttc tatccctaaa aactgaaatc caaataaatc ttgctttaaa
     4981 aataaatcca taacttgttt ttcttttatt tctaattgtt tttcatatgg cacagacaat
     5041 agtgtacatc ctccacactc tctaaagtgc ttgcaagttt cttcagtttc aagtggtgag
     5101 ttctctaata attctatcat cttaacttca gctttcttac ttctaacttt tttaactcca
     5161 gctctcacct tttgaccact tattccaccc ttcatatgga taactctatc tccaacttgg
     5221 cttaaagatg taccaccaaa ttccatttta tctacttcaa attctataac atcttttttc
     5281 ttcacagaca ttcctccaat ttactatagt atctttatta tctttcttca atcaatttta
     5341 cttcttcatc tgttaactct ctatactcac ctaactctaa actttcatcc aattttaaat
     5401 tgcccataga caatcttttt aagtatacta cttttttgcc tacgctttca aacattcttt
     5461 ttacttggtg aaacttacct tcatgtattg taagctctat ttctgattca tcatcacttt
     5521 ttaatatatt taactgtgat ggcatagttt tatatccgtc atcaagcact acaccttcta
     5581 aaaaagcttc tacatcttct tcagtaacta ctccatcaat ttttgcataa taagtcttag
     5641 gcacatgctt cttaggagat aaaactctat gagaaagctt tccatcatta gttaaaacta
     5701 ataatccctc tgtgtcttta tctaaccttc caacaggaaa tggttcaaaa gctaagtatg
     5761 ataaatcaat taaatctaat actgttgggt catatttatc tgtagttgct gatatatatc
     5821 catcaggttt attcatcatc aaatatatat attctctata tataacttct tctccattaa
     5881 ataatatctc atcattttca gtgtctactt gagttcctgg atttgaaact tcacttccat
     5941 ttactactac agacccttgt ttacaatact tctttatttc acttctgctt ccataaccta
     6001 aatttgaaag aattttatct atacgttgct ttttagccat taaaaatgct ctccattctc
     6061 ataatatttt tctttttcaa atactgtaaa tgctaaatcc atactttcta catctaaatt
     6121 atttcttata ctatcagtta ttattcttgc tactatatct tgtacttctt gtcctctatc
     6181 aaaccaagct acttcaacaa aaggatatac atctgctatt ttaccatctc ttattgcaac
     6241 tgactttata cattctattt caaagtagtc tcttggacat tttacagctt caactaaatc
     6301 atttatcatt ttttcactta ttttacatat atcattttca ttaattcctc ttatttttat
     6361 ctgtggcata gtttatctcc tttattttaa atttttagtg aattatcatc cacttaattc
     6421 ccttcgataa cgtttgaagt ggtaacttct tggtcaagac aactattgtt atcagtatct
     6481 ccaaactaat aaggtagttc ctacccttat agttagcatt atatagctaa ttttcctaaa
     6541 ttgtttatat tctttattat tttatttaat cctattgtct tctatttaac attatacttg
     6601 taatttatag ttattacaat ttactatata ctttttttga tttaacgtta taatataagt
     6661 gatgggtaaa ttttaaaagt taataggagg agtttaaaat ggcattaatt actacaaaag
     6721 aaatgtttaa aaaagcttac gaaggtggat ttgctatagg tgcatttaac ataagtgact
     6781 tagaacaatt acaaggtgtt ttaaaagctg ctaaagctaa aaattcttat gttatgatac
     6841 aagcttctat gtcagctgtt aaatatgctg gacctcatac attagttgaa atggttaagg
     6901 ctgcttctga tgaaataggt gtagatgtag ctcttcactt agaccatggt ccaaatatgg
     6961 atgctattaa aacttgtatt gatgctggtt tctcttcagt tatgatagat ggttctcatt
     7021 ttgactttga agaaaatgtt agaataacta aagaagctgt tgaatatgca cactctaaag
     7081 gtgttgttgt tgaagcagaa cttggtgttc tagctggaac agaagatgat gttacttctg
     7141 atgttcacaa gtatactcaa ccagctgagg cagttgagtt cgttgaaaga acaggtgttg
     7201 attcattagc aatagctatt ggaacttctc atggtgcttt caaattcaaa ggagaagcta
     7261 aattaagatt cgatatacta gaagaaatcc aaagcaaatt accaggtttc ccaatagttc
     7321 ttcatggtgc atctgctgtt gaccaaaacg ctgttgctac atgtaatgaa tttggtggaa
     7381 atatagctgg tgctaaaggt gtacctgtag atatgttaag aaaagcatct tctatggctg
     7441 tttgtaaaat aaacatggat actgacttaa gactagctat gactgctgct atcagaaaat
     7501 tcttagctga aaatccaaaa gaatttgacc caagaaaata cattggtgct ggtagagatg
     7561 ctatacaagc tgtagttgaa agcaagatag atgatgtttt aggttctgct aactctataa
     7621 actaatataa cttttaaata tttataaaag aaaagagatg acttaaagta attaaattta
     7681 tttttaagcc atctctttta agttgtataa attatgtatc ttataatatt ttaattatat
     7741 tgtcttttta aactgacagc taaacgtgaa tatgtccact ctttatcttt ctatggatgg
     7801 tagaaggata tatacctata acttcagcag cctttgttat tgaaccatac ttatttatag
     7861 ccttacttat cattatttgg tccactattt tatatgcatc attaagattc ataattccat
     7921 ttaccactat tggtgataaa tcatctgttt cattatcaat catatctaaa tttatcagca
     7981 tgttaaattc atcttctcca attacatttt tagctgataa tactacaaat ctttcaatga
     8041 tgttttttaa ttctcttatg tttcctggcc aagaatattc ttctaataac tcaataactt
     8101 taggcgaaac ttctacttct ctattgtact tttcattgta aagttttaag aaataattta
     8161 ctaatggtac tatatcctct tttctttctc taagaggagg tatttttact ggaataacac
     8221 ttaatctata gtacaaatct tgtctaaatt taaggttatt tctaaggtct tcatgagaaa
     8281 tattagtagc acttatatat ctaacatcta tttttatagg cttactacca cctattctag
     8341 taatagtatt ttcttgtata accctaagta attttttttg catctgcatt ggtaactcac
     8401 ctatctcatc caagaaaata gttccaccat tcgcctcttc aaaaagtccc tttttacctt
     8461 ttttcttagc tccagtaaaa gaaccttctt catatccaaa aaactctgac tctaataatt
     8521 catttggtat agctccacag tttatagcta taaagggttt gtctctcctt gaactatact
     8581 tatatattgt tcttgctatt atttcttttc ctacacctga ttctccattt ataaatatag
     8641 aagaatctga ggctgcagct ctcttaacaa ctttttttat attgtccata atcttacttt
     8701 gacttatata ctctgtgtct tctgatatat cactatattt tatctgagat tcaagcttaa
     8761 taacccttga aatatctctc cctgttagta ctcctcctgt aagctttccc ttagaatccc
     8821 atcttggaac tgccgtatat gcaataagct tcccatctgg gtaaacgata tttctataaa
     8881 tctttttctt cactttaagc atgtttaata ttattggttc ttcaactaat ccttgtctca
     8941 caaggtctgt tatatttcta ccaagtacct cagttctttt aaaaggtaat atctcatcac
     9001 tgacagtgtt catcttttca attaccccat ctttgttaaa cacaacaaca acttcatcta
     9061 tatgctctat tatatcttca attactttat cagatattag agacttctca aataaataaa
     9121 ctatattatt ttcattatta ttatctttca tattgtatat atgtgcagtc atccttaact
     9181 ctctgaaagc tattttcttt ttactatctg tcagtatttc tgttttgtca aaatttgaat
     9241 ctaattcatt tatatttcta ggctttttta tatctagaaa actaaaaata ctatctatta
     9301 gatgattaca gtatattatt tcgcctgatt ctttacataa aatggctgga ttttctacat
     9361 gttctataag ccctattaaa ttattatcca ttaatattcg cacctttcat taagtaattt
     9421 attgaactac atacaaaatt tattgtttgc ttacaataaa taccatcagc acatataatc
     9481 aactccttat aatattcgtc ttttcaagta gtttatttca ttaaaacgta actaagatat
     9541 aattttaatg attatagttt gttaaaaatt tcattttgct tttaattgta ttttatcaca
     9601 tatttagtaa ttatttgtct atttaataca atttaattaa atttaagatt tatttaaaaa
     9661 aacaaagata atccttacag aatataactc acaatttgta agcaatatac atataaggat
     9721 tatccagatt atcttgtatt ataaaatttt attcaaacag actttttaat aactgacaaa
     9781 tacacactag caataactta tcaaagggtt aataattatg ttactaacta ataagactta
     9841 ttcaatcagt aatatttata tagtttgtat ctattaagct tttatagctt ctaataaatc
     9901 atcaacgcta tctaattttt ctataagcat tggaactatt tcatgtaagt ctccaactac
     9961 tccatagtca gcgatttcta aaattggagc tgctgggttt ttgtttatag caattatgaa
    10021 atctgatgat tgcatacctg ctaaatgttg tattgctcct gatataccac aagctatata
    10081 taggtttggt ttaacagtag ttcctgtttg acctacttgg tgagaatggt ctatccatcc
    10141 agcatcaaca gccgcacgag aagaaccaac tactccacct aatttgtcag ctaatttctt
    10201 aagcatttca aatccttctg gtccacctaa tcctaaacca cctgatacta taacatttgc
    10261 atctgtaaga gatactaaat ctttcttagt tttaactgtt tctaaaacag tagttcttat
    10321 atcatcttga gttattttgt agtctaatgc tataacttca ccagttcttg tttcgtcttt
    10381 ttcagcttta tccataacac caggtctaac tgtagacatt tgaggtctat ggtttggaca
    10441 aatgattgta gccattatgt ttccaccaaa tgctggacga gtttgtttta ttttcttatc
    10501 ttctgggtct atttctaact tagtacagtc agctgttaat ccagttccaa ctcttgaagc
    10561 tattctaggt gctaagtctc tacctatatg agttgctccg aaaagcatta tttctggttt
    10621 tatttcatct attgcatctt ttattacttt tgtatatgca tcagttgtat atttttctaa
    10681 aagagcatca tctgcaacat aaactttgtc agctccaaaa gtgattaatt ctttagctaa
    10741 tccatcaaca tcttttccta ataatattgc acaaagttct gcatctactt ctttagctaa
    10801 ttttcttcct tctcctaata attctataac tactggagct atttttcctt ctctttgttc
    10861 tgcaaatatc catacgtttt tataagaact taaatctttt atatcattca tagtttgtac
    10921 tcctccaact tatataatgt gcttttgttt tagaccgatt attattttac tagctatttc
    10981 atcagcttta ccttctaaga tttctccttt tcctcttggt tcaggagtaa atgatctaaa
    11041 tactttagtt ggtgatgctt ttaaaccaac ttcttcttta tttacatcta aatcatcaat
    11101 tgtccaaact tttacatcat gtttatatgc ttttactatt ttatcaacag acatgtatct
    11161 aggtgtattt aactctttta ctgctgttaa aagtacaggt gtacttactt ttattaattc
    11221 gtatccatct tctaattgtc tttgaactat tatatctttt ccttctattt tgaaatcttg
    11281 tacatatgta acttgaggaa tgtctaattt ctctgcaatt tgtggtccaa cttgtgcagt
    11341 atctccatct attgcttgtc ttcctgcaaa tattatgtca tagtcaccta ctttagatat
    11401 tcccgctgca atagtatttg atgtagccca agtatcagct cctccaaatg ctctatcact
    11461 taataatatt gcatcatcag cacccatagc taaacactct cttagcataa agtctgcttg
    11521 aggaggtccc attgttataa ctgttatagt tacatcatca tgctcatctt taatttttaa
    11581 agcttcctct aatgcatttg catcatctgg gtttaatata cttggaactc catctcttat
    11641 aagagtacct gtttctttat ttattcttac ttcgttagta tctggaactt gttttacgca
    11701 aactaatatt ttcaaagtct atacctccaa tttcattaga ttatttatta ttttaatacg
    11761 tttgatgaaa ttaccatttt ttgaacttct gatgttcctt cgtatattga tacgattcta
    11821 gcatctctgt acattctttc gatttcataa tcttttataa atccatatcc accgtgaagt
    11881 tgtaatgctt tataagcaac ttcatttgca atttctgatg catagtattt agccatagca
    11941 gattcttgag caactctcat tcctgcatct ctcttttgtg ctgcatcata aactaatcct
    12001 ctagctgcac aaacttttgt ttccatgtca gctatagtaa attgagtatt ttggaattta
    12061 gctataggct taccaaattg aactctttgt tttgtatatt taacagcttc atctaaagca
    12121 ccttgagcta ttcctagagc taaggcagca acacctattc taccaacttc tagagtaccc
    12181 attgctatct taaatccttg accttctttt ccaagtaagt tttcttttgg aactttaacg
    12241 ttttcaaata ttaagtcaga agtttcagtt cctcttatac ccatcttatc ttcatgagct
    12301 cctgttgaga aaccatccca tttactttct actataaacg ctgatgttcc tctaagtcct
    12361 ttacttctat ctgtaactgc tattactata gctacatcac agaatggtcc attagttatg
    12421 aaagtttttc taccatttaa tatatagtgg tctccaacta attctgctgt tgtttgttgt
    12481 ccacctgcat cagaaccagc tcctggctca gttaaaccaa atgctcctat taattcacct
    12541 gttgctatac cttttaagta tttttgtttt tgctcttcag taccattgta aagtattggt
    12601 aatgaagcaa gagaagaacc agctgtaaca aaagtagctg ttgaagcaca tcttctagct
    12661 atttcttcca ttactatagc gtaagaaaca tatcctgctc ccccaccacc atattcttct
    12721 gggatattac tgcttataaa ttttgcatca gctaattttt tgattaattc cttaggcatt
    12781 acacctgttt tatccatttc tgctggtaaa gtatctaatt ctttactaac aaaatctctt
    12841 actgcttttc ttaaaagttc ttgttctttg ttatataaca tagcttcctc caattttcat
    12901 attttatagt aaatttattt taatgaactt acgctaaact catcatctca gcaaaagttt
    12961 gaatacgagt tctggcttgt tcattattct gagtttgttg gtcgatttca acatataaag
    13021 taggtattcc actatcttct atatcttttc taactaaagg ataatcgtat tcttctgggt
    13081 cacagaattt catcatacag aagataacac catctatatc tttcttttta acttcatcta
    13141 ctataagtga cccacgtttt ttcttagggt cataagctaa tgaacatcct tctatgtttg
    13201 accattgtct tgctaatctc tctaacgcat catcacctgc tggtacatct gttctgaatt
    13261 gtcttgtttc ttgtgctaag tcgtcagcta caactgatat attgttgtct tctaaaatgt
    13321 ctaatatatc tttagaatca gctaatatac ctgttaataa aactttcttt ccagaacaga
    13381 cttcttctgg catagcattt aattttgcta ttaaatcttt cactagctca gtatgttctt
    13441 cttttctcat aaagaaccca ctcttaatta ctaagcttct tattgatggt tttatagtat
    13501 tagaatgttt ataagctact tcaacaaagt ctctcatagt ttttctatgt tcattgtaaa
    13561 cttctatact ttcatgaatt tttgcttctt ctatttcata tccacaaatt tcttcaagtt
    13621 ctctttttac acctttgtac tcactgatta agtattttac accagcttca agttttctat
    13681 tttgtgggtg tactaatgat atatatttta tatgaggtac tgctgatttc cagttttgtc
    13741 ctaaacaaat tagtgtatca cacatacctg gtataataac tccagataat tcatcataag
    13801 caccttttaa tccatattct aaacatgatt gcattattga acatgcaaat gcagggaaat
    13861 attgtttagc taaatctaat tctgtttgtc ctccccatat accaactgga agcattccag
    13921 ctgcatgtat aatttcttct gggcaataaa ctgggaaaca acctatagct tttttaccag
    13981 tttcactttt atattttttt acagccgcat ttggattttc aactacttct ttcattttag
    14041 ataaaatagc ttccattata tctcacctct attaagtttt tttctttctt ccattacttc
    14101 aactaaacct tgaattcttg tttcaaattg agcattagtg aaagctctag ggtctgcttg
    14161 gtcaccatca aatccagcat atggtaatcc agtttcttca gctgctcttc tttgcatttc
    14221 atattgtatt aaactcataa gtttacagct tctgttcata tgatagaatg ctccatcaca
    14281 tttaccctct actaaagaat caactctata ttttgtcata cggtctaggt ttacattgtt
    14341 aaacatagta ctatatgcta ctgccattcc atctaaatca ttaacttcat attgtaatgc
    14401 ccaagcatgt gggtaaacac tacctgtcat gttaactcca aatttagcta atgttttcat
    14461 cttgtatcct atatatggcc aacaaggtat accttccatc attattctgt atttttcttc
    14521 ccctctgaaa gaagatttac cagttttcat gttgtcctct aattcttcta taagtaactt
    14581 aaatgcttct gtagtttctt ttttacctct agcacaaact attacagcca tgtaagtaaa
    14641 taagtcaaat ccattcattg gagaaggaga agaatctgct ggtaaactca tagaatactt
    14701 ccatagtctt ccgttttcag ctgatatttt cattacttct tcaaacttct tagggtcaaa
    14761 tttctttcct gatataattt ctagttgttt tatagcttct tcaaattgag ctttaatata
    14821 atctattctt gattgagtaa cttcgtcttc attattgaaa gttgtatcaa tcattattaa
    14881 aggtatatct aattctcttg aaatattttc ataccatttt ataacttggt tacatatatt
    14941 gttacagcaa agtaggaaat ctggagccgg catatccaaa gcttcacatc caccattttc
    15001 taaaagacca aaatttgttc ttgcatatgc acataggtca atagaatatc ctttagattc
    15061 agctatttca cataattcta aagaaccttt tttagctgct actccagctg cttggttttc
    15121 tggatataat acgtttaagt caaatacttc tgctaactct tgaggaaata ctgatgttga
    15181 ccaacctaca ggtcttcctt cttctttagc tttaaatgca tttgcatatt gttcagctaa
    15241 taaatcatta attactactc tagcttcttt tttttcagac attttcatcc ctctttttat
    15301 atatttttca cttctttttg tgattctttt gcttcatcaa aagcatataa cgctgctcct
    15361 aaagcaccag ttaattgagg tatatcaggt acaataattt ctgtgttgat ttctcttgcc
    15421 atagctctta caataccact atttctagca actccaccaa ccataactac atttctttgt
    15481 actcctattc tttttactag gctagaaact ctctttgcta ctgaagtatg aatacctgcc
    15541 actatatctt caatttttgc attttcagat aaatgtgata taacctcaga ctctgcaaat
    15601 actgtacatg tactgcttat tgatacttca ttttgagaat tcatagatat acttccgagt
    15661 tcagatacat caacctctat tatttttgcc attacatcta aaaatcttcc tgtacctgca
    15721 gcacatttgt cattcataag aaagtttaat agtcttccat tattatctaa ttttaatacc
    15781 tttgcatctt gaccacctat gtcaataatg gttcttgtct ctggaattat gaaatttacc
    15841 cctctagcat gacagcttaa ttcacttatt tgcttatcag catctgaata gttcattctt
    15901 ccatatcctg taactacaac ttttttaata tcttctcttg caagacctgt cttgccatat
    15961 aatttttcta aaactcttga tggtccagta gtcccagtac cagaggatat tgtttcagaa
    16021 gctacaatat cttccccatt ctttaagatt actccctttg atgcagttga acctatatct
    16081 aatcccattg tgtacatctt tagcattctc cttttaaaaa atctagaatt actaattaat
    16141 atcttacaac tttactatct ttaaagttat ttattttctc ttcatcgtag cctaaagatt
    16201 ttaatacttc tacagtatgt tgacctactt ttggtgctgg tgtatattct ttaattcctt
    16261 catttctaaa cataactgga gtattaacta agacacctgt atttccgcta tcgtatgttt
    16321 tcttaaataa gaagtcattt gcccaagctt gttcgtcatc taataaatct tcacagcttt
    16381 gaattttttc aaatggtaag tctgcttctt ctaataaagc tgaccactcg tctaatgttt
    16441 tttctaacat agcttctcca actatcttaa ctaaatcttc aacatgatta accattgaat
    16501 ctatgttatt atatctatcg tcttctaata tatattctct atttataacc ttacagaatt
    16561 tgcctaacca cttgttgtat tgtattaaag ctaattgaat ccatcttcca tctttacatt
    16621 tatatgtagt cattaatggg ctgtttggat tttctcttga taaaggcatt tcatttccgt
    16681 attgtgctgt tgttatcatt gttcccattc catatatagc tgtatggaaa agacttactg
    16741 ttactctctc acctttacca gtttgagctt ttttatgtaa tgctgctaaa cttcctgctg
    16801 ctagtgctag acctgcatag tggtcaccaa atcctgctgc tgtatttgct ggagatgttc
    16861 ctttttccat aacagattgg ctaactcctc ctcttgcgaa gtatgcagta tagtcaaatc
    16921 ctggtttatc ttttaaaggt cctttttcac catatcctaa tatttgagag aatattaatc
    16981 ctggatactt atcttttatt tggtcataag ctatacccat tttttctaat gcttgaactc
    17041 taacattagt tacaaatatg tctgcttctg ataataattt atgtaatatt tctactcctt
    17101 cttttgattt tacattaata cttacaccct ttttatttcc attttctaat tcaaacatag
    17161 ggttttcatc atctgatgct ggagatttaa atgttccacc cataactctt attccatcac
    17221 cttctatagg ttcaatctta ataacctctg caccccagtc acctaacatt tttgcacaac
    17281 atggtgctgc gatgaaactt gaaagttcta ctactttaac tccttctaaa agcataacgt
    17341 tttcctcctt aatagtatca attataaaat ttcgattaaa tcactcataa aatacttttt
    17401 tctgatatta actgtctgat gtcaaagttc ctaatcttat ctaaaataac ttaaaaatta
    17461 ttaaaattat tttttattgt tttattgtct tacacctata ttgttaagca tttatcgtgc
    17521 caactttata tttaatcaaa atatcgcttg atttgaatgt tttttaacaa tatgcaattg
    17581 aataatcttt ttcatatcgt ggcatcttgc cactatccat tgcattttgc tattctttct
    17641 atgcttataa aagttttttt tatatatttt tttatgaaaa agcttgacat ttcaaaaaaa
    17701 gttttcattg ctaaactcaa aaacacatgt tatcattgta attaatcagc aagttagtta
    17761 actaatttca aacttagata attttttaat aatatttaat ctaaaaaata acaatttctt
    17821 tgataaattt tagagtaatt attacattaa ataaaaaatt actaaatatc aaaataacac
    17881 agggaaaaca atttcaaaac taaattttta accttaaaaa ttttgtataa aaaatataac
    17941 cttatatgct acatattatt tctaaatgaa aaattaccat aataatttaa taattacagg
    18001 agggtattga tatgaaaata ctagtatttg gagcacgcga ttatgaagaa ccagtaataa
    18061 aaaaatggtc tgaagaacat aaggatgttc aagtggatat ttatcctgaa aacatgactg
    18121 aagaaaatgt agttaaagct aaagggtatg atggtatatc tatacaacaa actaactata
    18181 tagataatcc ttatatttat gaaactttaa aagatgctgg ggttaaagtt atagcttcaa
    18241 gaactgcagg ggttgacatg atacattttg atttagttaa tgaaaatgga cttatcgtta
    18301 caaacgttcc ttcttattca cctaatgcaa tagctgaatt agctgttact caagctatga
    18361 accttttaag aaagactcct ctagtaaaga aaaaagtctg tgaaggtgat taccgttgga
    18421 tagctgaact tcttggaaca gaagttagat ctattacagt tggtgttata ggtacaggaa
    18481 aaataggtgc tacttctgca aaattattca aaggcctagg agctaatgta attgcatttg
    18541 accaatatcc aaatagtgat ttaaatgata tattaactta caaagattct ttagaagacc
    18601 ttctaaaaga agctgacctt ataacattac atactccttt acttgaagga acaaaacata
    18661 tgataaataa agatactcta gctataatga aggatggagc ttacatagta aatactggcc
    18721 gtggtggttt aattaataca ggggatttaa tagaagcact agagtcagga aaaattagag
    18781 ctgctgccct tgatacattt gaaactgaag gattgttctt aaacaaaaaa atgaatcctg
    18841 gagaattaac tgacccagaa ataaataaac ttctttctat ggaacaagtt atattcactc
    18901 atcaccttgg tttcttcact agtacagcga ttgaaaatat agtttattct agtttaagta
    18961 gtgctgtaga agttataaaa acaggaactg ctactaatag agtaaattag tattttataa
    19021 atatattttt tcattaatta gctattatct aacaaaatag cctttaattt cccaaatata
    19081 agagagtcag ctcagtatat attgagtaga ctctcttata ttatttttta tattaaactt
    19141 gtactcttct tattcatacc aaattaaatt tatattaact tactctaact actcctactc
    19201 tatacatttt acctattttt acttttctaa ttatatacat ttgaatgggt acaatctgta
    19261 ttttacaaaa aaaacaccta acaaactatg aaaattgtat gattaatatt aatatattta
    19321 taagaaaaca taacaattat gttagctctg gatttttagg gagggagtat attggaaaaa
    19381 atgaataaag ttacttctaa aaaaactacc tatattgtta cttcagcatt atttgcatcc
    19441 ataatatgcc tgacaatagc atatatatta catattccag ttggtggaaa caatggatat
    19501 gttcatatag gagatgcttt tatttatcta gctgcaacta ttttgccaac aaactatgct
    19561 atagctgctt ctgcaattgg tgctggtcta gctgatttat ctacaggtgc tgctatctgg
    19621 gtaataccaa ctataatcat aaaaccaata cttgtattat ttttcacttc taaaagtgac
    19681 aagataataa ataagagaaa tatagttgct tcggttgtag caggtatagt aggattagtt
    19741 ttatacatgt ttgctgaagg tataattatt ggaagtttta caagtgcatt tgtaatgagc
    19801 ttacttggct tattacagcc aataggaagt tttattgttt ttataatctt aggtatggca
    19861 cttgataaac ttgattttaa aaaacgttat tttaattaag aatttaaatt tatggaggga
    19921 aaattatgaa tatcttatat acaattgaaa atagtatcta tgtaaatata actaatacat
    19981 gtccatgtag ttgtgttttt tgtataagaa acgaaaaaga tgaagttgca aatagtggta
    20041 gtttatggtt ggagcatgag ccaagtgtag atgaagtaaa agaagccttc aataaataca
    20101 atttagacga ctatgatgaa atcgtatttt gtggatatgg cgaaccatta atgagaataa
    20161 atgaattgat agaggtagca aagtttataa aagaaaaaag tagcataaag ataagaataa
    20221 atacaaatgg tctaagtgat ttaatacata acaaaaaaac agctattctg ttaaaaaatg
    20281 ttatagatgc agtatcaata agtcttaatg ccccaaataa agaagcttat aatagagtta
    20341 ctcaaccaaa gtttggagaa aaatcatttg attatatgtt agattttgca agagattgca
    20401 aaaaatatat aaaagaagta gctttttcag tagtagatga aatatctcca gaagaaatag
    20461 aagaatctaa acaactggca aaaaaacttg atataccatt aagggttaga cataagaatt
    20521 agcaagtaaa attataaaac attaagaatc acctgaatgg tctttcaggt gattcttcct
    20581 cttgtattaa cattttacaa aatagatttc ataccacact aggaatgtgt aaatcatcca
    20641 aatctttctc caattatcag agtttccact aatgtagtca ttaaaaatct cattaatctc
    20701 atctatgtta aagaatttat ctgctatctc actattgaat ttatctcgga tatccttatt
    20761 atattggtca tcagccatcc atatacgaat tggcacaata aatcctaatt tatttctaaa
    20821 agcaatctct tctggtaatg tttttgatgc agctgtacga aaggcaattt tattctcttt
    20881 tcctttcact ttatattctg atggtattct ggatgcaata tcaaatattc ttgtatctgt
    20941 aagaggcatt ctgacttcca atcctactgc agtactcatt ttatctatat taaaataaat
    21001 atctccttcc aaccatattt gaatatctac atttgacatg ttagtaagtg tatctagttc
    21061 ttctgtttct tcataaattc cttttaccac ttcaatagga agcacatcag gattgtattt
    21121 cttaagaatt tttcttttct caccctcatt cataatgttt gtatttccaa tataaaatgt
    21181 cttcaaattc tctccataac actctgcctt atggtatata ttatatccac caaaaaacgc
    21241 atctgctcct tctccagagt aacagatatt tgtgttttca gctgctgcct tgcatccaat
    21301 agcaaaaacg atagcagatg catctccagt tggttgctcc atatggtaca ttacataagg
    21361 tactgacgca aaatactctt caggtgtaat tatatatctc gaatttttac acccaagaat
    21421 attagctgtc tgttgtgcta attttgattc atcaaaacgc tcttcctcat aaccacatga
    21481 acctgttttt tctatatctg acattgccaa tatataagat gaatcaacac ctcctgatag
    21541 gaataactct gcagtttcat tctctgattt tacctctggc ataattttct gaagtgttgt
    21601 atgaatttca tccgtccatt cctcaataga cttgctgtgg tcaggatgaa attcaggttt
    21661 ccaataacga ccaatacata ctgttttatt ctgatagaca aggtaatgtc ctggcataag
    21721 cttctttact ccacggaaga atgtattttc acctgctaca tatgtgaaag acatataaat
    21781 ctgtaacatg tcttcattta actctttcac aaatcctggt tgctccataa tcttacgaat
    21841 agatgaacca tagagaagct tcccattttc agtctcataa taatagaaag gttttacccc
    21901 aaactggtct cttaggcaga agagtttctt ttccacagta tcccataatg caaaagcaaa
    21961 cattccatgc atgtgatttg ccatatcata tccccatgtc ttatatgctt ccactaaaat
    22021 tgctcgttca cgctcatctc tacataatga agaatcgatt ccaagttctt cacaaagaac
    22081 tttccagttt cgaatatgcc cattgtattc tagaattttt atcatatatg tgtcttttcg
    22141 tgttaaatac ttttcttcta aatttatcat atgtgtatcc tttcgtatta aaacatttaa
    22201 cttctaaatt tattaaatgt ttatatatat ttattcactt ataatcactg aaaaatcgac
    22261 ttttttcaaa gtaaattctt tttgaattag tactaatctg ctctttatta atggcataaa
    22321 tatgattatc aagtattatc agtgttataa cattgaattt tttattcttc ataaatattg
    22381 cccccttta
//
LOCUS       DAMJVI010000059        22309 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.061, whole genome shotgun sequence.
ACCESSION   DAMJVI010000059 DAMJVI010000000
VERSION     DAMJVI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.061"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(138..1328)
                     /locus_tag="PC545_002307"
     CDS             complement(138..1328)
                     /locus_tag="PC545_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2457074.1"
                     /translation="MNFNQKRIAASIMATAIIMPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKEGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTNENAENSSNDLVKGTKVVTAKSLNVRTGPGTSHSKIATL
                     SYGTEVGSISESGGWTKISYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGADSVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSVGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLVFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(1588..2019)
                     /gene="dut"
                     /locus_tag="PC545_002308"
     CDS             complement(1588..2019)
                     /gene="dut"
                     /locus_tag="PC545_002308"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDF2457075.1"
                     /translation="MYNLKVKKLNDDAIIPNFAHKGDAGMDLYSIEEVVIPPGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIVKPIYDINIEEVKELSDSERGKGGFGSTGF"
     gene            2132..4360
                     /locus_tag="PC545_002309"
     CDS             2132..4360
                     /locus_tag="PC545_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDF2457076.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFVPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAEAGEYKGKYVKGKESKFDTLEDANKIIASITSETGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKSLSKDEQLVYDA
                     IKDRFVANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKISQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEAYTFDSKTKKFTKTKEKKQKKDVTNT
                     EKEVAISKDSNNKKEVKNGDENQSLGKCPICQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDATLKYDKNPDTGYFNWKIEF
                     DN"
     gene            complement(4471..4956)
                     /locus_tag="PC545_002310"
     CDS             complement(4471..4956)
                     /locus_tag="PC545_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2457077.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFKESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFKLFDAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
     gene            5208..6431
                     /locus_tag="PC545_002311"
     CDS             5208..6431
                     /locus_tag="PC545_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDF2457078.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEESEPEKPIAVSVQKAIGGEIE
                     NTNSFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPELENSVKQSK
                     ASLDLAKASYSSATGGSLEAQVNQAKTALDNAKIQYEESQRNYDNNKILYEQEVISLD
                     QFKKIEFSLEQTKQQLDSAQRAYDTATSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTSPVDGTITAKNFDSKEMITQSQPAFIISNPNILEVDLNVAESDIGKFKKDGNVD
                     VIIEDQRILGKIDYVPSVVDPQTSLYPVKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEENGKKYVYIATKDNIAKKHLVQTGIVTEDKIEIKSGVSDKDTVIIGGIS
                     LISDGTKIFPVEKEK"
     gene            6435..9569
                     /locus_tag="PC545_002312"
     CDS             6435..9569
                     /locus_tag="PC545_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDF2457079.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQVSQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     SNLLISAVIAFIVIFAFLKSVRASLVVAVAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSLDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTEGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDSPIFDK
                     VSEAYKKLLSVCLRHQRLVVISSLLIFVASLFGMGFIGMDFMPAADKGELSISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNSKASIAIELESQKNRKRTTDE
                     IEKEIIDRVATVPDCKINVSQNSSVMGGAGGADFTLEMKGPDLDVLKILGKEAEKEFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGVNTAEIAGMLRTAINGDSVTTATIDD
                     YKVDVNLKFKADTIDSLEDIKQLKVNSATGKEVPLGAFSDIKMADGLQSISRTDGDFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGETEMMNESMASLGFSMVIAI
                     ALVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTVSTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGSI
                     KRKIIKKPKLKAKV"
     gene            9603..10901
                     /locus_tag="PC545_002313"
     CDS             9603..10901
                     /locus_tag="PC545_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDF2457080.1"
                     /translation="MSKIVAISCGVGIITANLIPVYADNNTSSINKVDNSILSDSNKR
                     TSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFPYDQI
                     GLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMKLKKD
                     LGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEYRFES
                     FRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVAPTKP
                     EQGNLTDDEYKVLMDKYEKDYDRYKKEVEAYNLERQTYAAGLTTYANYLQQKFNTENG
                     LVTLEDSKKALKKGLIDSYAQLLALEDTIQITKKQLDLSEKQLKNTKLRYDLGLITLT
                     DYKKQVVSNEDAKNSYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(11190..11435)
                     /locus_tag="PC545_002314"
     CDS             complement(11190..11435)
                     /locus_tag="PC545_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2457081.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKK"
     gene            complement(11649..14276)
                     /locus_tag="PC545_002315"
     CDS             complement(11649..14276)
                     /locus_tag="PC545_002315"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HDF2457082.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRAEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDKALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGIEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            complement(14337..15179)
                     /locus_tag="PC545_002316"
     CDS             complement(14337..15179)
                     /locus_tag="PC545_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDF2457083.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGVLQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            complement(15186..15824)
                     /locus_tag="PC545_002317"
     CDS             complement(15186..15824)
                     /locus_tag="PC545_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457084.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            complement(16260..17552)
                     /locus_tag="PC545_002318"
     CDS             complement(16260..17552)
                     /locus_tag="PC545_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2457085.1"
                     /translation="MLDLNRKLAKLEEEGKKIKVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNEKDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAERKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLDHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEFMDGYGAYTCYGTIEKYDVAKAMNAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            complement(17589..17945)
                     /locus_tag="PC545_002319"
     CDS             complement(17589..17945)
                     /locus_tag="PC545_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDF2457086.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     IATLKSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKCEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            complement(17980..18339)
                     /locus_tag="PC545_002320"
     CDS             complement(17980..18339)
                     /locus_tag="PC545_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457087.1"
                     /translation="MKLLVILFVAYILNTIFIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSDGFIKFGEILKGRTVMAKFKEIEEIEGLDIYSAEQKFKDEES
                     IVQAIKFIKEKINVSFN"
     gene            complement(18408..20381)
                     /locus_tag="PC545_002321"
     CDS             complement(18408..20381)
                     /locus_tag="PC545_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2457088.1"
                     /translation="MSVTEKIPSIDRKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAVIKYIKGQGYKLEIKDEQILNKIIDMNRKNVFSLNSKEDRVEF
                     ILNYLIELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFSNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKEEKKVKDYDVKFDLLKSYDEYFIAREIKHIISGMFECTLNDEE
                     IIYLTIPLVSGNAPASECALNSSRISKNIDELMENIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILASLEKMMDHLMDLGCIDDKFRKNIVNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIEYANRSIKIILMTMFPCENKIDSDLLVKIYE
                     EVLKIGQDVKLTNKISKCRTFLEFKKVLLKNLI"
     gene            complement(20603..21616)
                     /locus_tag="PC545_002322"
     CDS             complement(20603..21616)
                     /locus_tag="PC545_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDF2457089.1"
                     /translation="MEENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEIVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVNPNCISVVDGEDMPQKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     ARVISPLAGNIGGLLIISVICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPIAVIIAYGVAVF
                     ALR"
     gene            complement(21629..22174)
                     /locus_tag="PC545_002323"
     CDS             complement(21629..22174)
                     /locus_tag="PC545_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDF2457090.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNRATNKA"
BASE COUNT         7646 a         3726 c         2883 g         8054 t
ORIGIN      
        1 agagaaaata aataaaaagc aggtatcaat caaatagtaa catgtattat actatacatt
       61 actatcaata ttgtacctgc tttcatatta taagcaataa agaaaaatct atttattagg
      121 ttttataata aaaacaatta taaaactctt cttgcattta catatctact tgaatagtag
      181 ctactattta tattagatat tgttactttt tttgatgaac ctgaagatgc atgtatcata
      241 tctccatttc ctatgtatat tccaacatga cttacagaac cattatttga accttgtgta
      301 tcaaagaata ctaaatctcc acttctaagg tctcctctat ttacatatgt tccatactta
      361 ctttggtctc tagatactct tggtatacta actcctacag attttttcat aacgtactgc
      421 gtgaatccag aacagtcaaa actatttgga ccttctgctc cccatacata tggttttcca
      481 agtagtgtct ttgcaaaact tattacacta tcagctcctt gacttggaga attagtcgaa
      541 taagatggaa ttgaagtatc aacagaacct ttttcagcaa ggtattgact tgacacatat
      601 ccagtttgat tgccataact tatttttgtc catcctccac tttcagaaat acttccaacc
      661 tcagtaccat aacttaatgt agctatttta gaatgacttg tacctggtcc tgttctaacg
      721 ttcaatgact ttgctgttac aacctttgta ccctttacaa ggtcatttga actgttttca
      781 gcattttcat ttgtagagtc atttacacta gttcctaaat atttacttga aacataacct
      841 actctaccat tagaacttat ttttgaccat ccatttgact cagatatgta tccgacttca
      901 gtcccatatc ctagtgttga tattttagaa ctacttgtac ttggtcctgt tctaaaattt
      961 aatccttttg ctgttacaac ttttgtcgac ttaactgaag tatcactact ttcattgcta
     1021 cttccaaggc tatttattgc tacataattt ccatgcacat aacctgtgtt tccattatat
     1081 ttaactttta cccaagaacc ttcttttcct acgtactcaa ctttatcacc tttgtaaagt
     1141 ttccccattg attcatggtt tgtacctggt ccttttctaa aatttaccgc attccctgtt
     1201 attgttctgc tttctatact cacactctct acttcagact cattagcata tgctaaattc
     1261 cccatagtag gcattattat cgcagttgcc attatactgg ctgctattct cttttgatta
     1321 aaattcataa ctttccactc atctcctctt ttctactatc tttatttcac ttcaaaatat
     1381 aaaagaaata attgacacta tcaaaatatc aattatttct tttatattaa tattattaca
     1441 aatttgtaac tatttgtcta tagtaatatt tgcttaccat aaattaccat gtataaattt
     1501 taatctttaa tagcctgtaa tttagtaata tagccatctt ttaccttttt taaaaatggc
     1561 tatatttttt aacttttttg taacttttta aaatccagtt gagccaaacc caccttttcc
     1621 tctttcggag tcacttaatt cttttacttc ttctatgtta atatcataga taggttttac
     1681 tatcatttga gcaattttca tatgcttttc aaccttaaaa tcatttttac catgatttat
     1741 taatataatt ttaagctctc ctctgtatcc ttcgtcaata gttcctggtg tatttaatac
     1801 agtaacagaa tgctttaagg caagcccact tctaggtctt acctgcgctt ctgtcatagt
     1861 aggaagctct atacatatcc cagttttgat aagtttagtc tctccaggag gtattacaac
     1921 ctcttctata gaatacaaat ccataccagc atcacctttg tgagcaaaat taggtattat
     1981 agcatcatca tttaattttt ttacttttaa attgtacatc ttatatctcc tttcaacttt
     2041 ctataaatat tataacttaa atttcgacct cttgaagttt tttatttttt tgtggtaata
     2101 taattattag tgttttaaag gaggattttt tatgaaactt gtacttgctg agaaaccatc
     2161 tgtggcaaaa actatagcat catttttagg tgcaaagacc agacaagatg gttactttga
     2221 aggaaatgat tatataatta cttatgcagt tggacactta gtatcattat atgatatgaa
     2281 ggattatgat aaagataaat actctggctc ttggaaaatg aacaatttcc catttgttcc
     2341 tgaggataag tttaaattta agattgatag ttctaaaact aaacaattca acactgtaaa
     2401 aaagcttttg aatagaaaag atgtagaata tgtaataaat gccactgata atgaccgaga
     2461 aggagaactt atagcatttt taatattttt actggcaaaa aataaaaaac cagtaaaaag
     2521 aatcttggta aatgaatgga caccagaaga tattacaaga ggtataaaaa acctaaaaga
     2581 tgaagatgaa atgagaaatc ttcaagctgc tggatatact aggcttatta ctgactggtt
     2641 gattggaata aatttcactt ctgttgctac attgaaatac ggaaatggga aactattaaa
     2701 cataggtagg gtaattcttc caacagttaa attagtctat gatagagata tggaaatact
     2761 aaactttgtt cctaaaactt attatgaaat agaagggcat tttaaagctg aagctggtga
     2821 atataaaggt aaatatgtta agggtaaaga aagtaaattt gatacacttg aagatgcaaa
     2881 taaaattata gcttcaatta catctgaaac tggtaaaata ctagataaaa aagttactat
     2941 gtcaaaggaa tatgcaccaa aactttttag tttgacatca cttcaaggat atataacttc
     3001 taagtattcc aattttacat ctgataaggt tttaaatgtc tgtcaatctc tctatgaggg
     3061 taatggtaag ggtggataca taacttaccc tagaactgat tccattttct tagaagaaag
     3121 tttggcttca aaagcttctc aaactttaga taaattaaag gttggtcttg aatatgaaaa
     3181 taaaattaaa ttttctaaaa ctaaaagagt ttttgattct tctaaagtag actcccatag
     3241 tgctataatt ccaacttata taattccaaa aagtttatct aaggatgaac aattagttta
     3301 tgatgccata aaagatagat ttgtagcaaa ctttatgcct cctgctgaat atgagaatac
     3361 agaaattaag actgaagtag ataattgtac tttcttaaca aaaggtaagg tgcttaaatc
     3421 aaaaggatac cttgaggtat ataataagga agaaaaaaac gatttacttc cacttgtaaa
     3481 taaagatgat gttgtagatg tgttagaaat aaaaccatta actaagcaaa ctactccacc
     3541 taaaccgtat actgaggaca cacttttaaa ggctatgaaa aattgtggta aaaatgtgcc
     3601 tgaagaagat acaacagtgc tatctggata ttcgattgga acatcagcca cacgtgctga
     3661 tgtgttaaag aagataagtc aagtgggcta tgtaaagaaa aaaggtaaat cttattctat
     3721 aacagacctt gggaaaaatt tagttgaaat ttttcctgtt aaagacttat ttgatgtaga
     3781 ttatactgga aaacttgaaa aaagtttaag cgatattcaa aaaggaaagt atacaagaaa
     3841 agaatatctt actaatatca ttagttttat atatcaaaat gtcaatatta taaaacaaga
     3901 tgcttcaaaa aatataaaca cagaagcata tactttcgat tcaaaaacta aaaaatttac
     3961 taaaacaaaa gaaaaaaaac agaaaaaaga tgtaactaat actgaaaaag aagttgctat
     4021 atcaaaggat tctaataaca aaaaagaagt taaaaatggt gatgaaaatc aatctcttgg
     4081 caaatgcccc atttgtcaag gtgatgtact agaatttgac aaaggctttg cttgtaaaaa
     4141 tcataaagaa tgtaaatttg ttatttggaa aaatgataag tatttaagtc tttataaaaa
     4201 gaaagttaac aaaacaatgg tcaaaaatat tttaaaaaaa ggtgaaacta aagtaaaatc
     4261 tttaactgct aaaaacggta ataaatttga tgctacatta aaatatgata aaaatccaga
     4321 tactggctat ttcaattgga aaatagagtt tgacaactaa atattcataa gaaaagaaat
     4381 agcagtagta taataatcta tattatatta ctgctatttc ttttcttatg aagtttcaac
     4441 ttttttaata gtgactttta caatcacact ctaacctaat ttctttacta tttctgataa
     4501 ctttaatgta tgttcatcta ataaagttag ttcctcttca gaaagattag ataatttttt
     4561 tactagagat tctttgagaa tagcttttac tgcatcaaat aatttaaatg ctttatctgt
     4621 taattcaacc ctaagaatcc ttctatcttt tgggtcagga aatttattga ctaaaccatg
     4681 ttccactaat ctgtcaataa tcggagtcat atttgatttt aaaattccta aattagaagc
     4741 tatttgagaa attgatgatg aatttgatgt cataagataa agaattacct tagcatgtga
     4801 tggagccatt ggacattctt caaaataagc tttcattata tcagagttat tttgaaactt
     4861 ttttaacaaa tcgctttctt taaatatatt attttcaagc acaaataaaa aatctaaaaa
     4921 agagtccgct attttaagta tattgttgtt atccatattt gtaacatccc ttcaatagat
     4981 aatataataa aacattgttg tttaatatag tataaattat aaactattaa taaatttaaa
     5041 acaagaaaaa gttattgact cataataatt ataaatttat aatcaaatta gagaattata
     5101 aattgccaat aaagggaagt tttttaatta gtctaaaact aaacttaaat tataagacac
     5161 tttaaattct acaacaagct agtgtgcaat atataatagg aggagatatg aaaagatatt
     5221 taatagtttt cttaatgtgt agttcactgt ttttagttgg atgtggaaaa acagaagaat
     5281 ctgaacctga gaaaccaata gcagtttctg ttcaaaaagc aataggtgga gaaattgaaa
     5341 acacaaattc tttctcagga actacaaagg ttaaagatga aacagctgtt actgcacaga
     5401 ctgttggcac agttcaagaa gtgtatgtaa aacttgggca aaatgtaaga aaaggagatg
     5461 aattattgag tataagcagt ccagagctag aaaatagtgt aaagcaaagt aaagcctcac
     5521 tagatttagc aaaagcaagt tattcaagtg ccactggtgg ttcattagag gcacaagtaa
     5581 atcaggcaaa aacagctctt gacaatgcaa agattcaata tgaggagtct caaagaaatt
     5641 atgacaataa taagattctt tatgaacagg aagtcattag tttagaccaa tttaaaaaaa
     5701 tagaattttc tttagagcaa acaaaacaac agttagattc tgctcaaaga gcatacgata
     5761 cagcaacttc aaaaagtata cctcaagcta aagcacttgc taaaaaacag cttgaccaag
     5821 ctcaagtatc atataattta gcaatgagta atttagacaa attaacatta acatctccag
     5881 tagatggtac aataacagct aaaaactttg attcaaagga aatgataaca caatcacaac
     5941 cagcttttat tatttctaat cctaatatac tagaagttga tttaaatgta gcagaatcag
     6001 atataggtaa atttaagaaa gatggaaatg tagatgttat aattgaagac caaaggattt
     6061 taggaaaaat agattatgta ccttcagtag ttgaccctca gacatcttta tatcctgtta
     6121 aagtacttgt aaataataca aacaacaaat ttaaggcagg tatgtctgct caagtaaact
     6181 taagtgttga aaaagaaaat ggtgctgtta cagtacctaa aaaagcaata tttgaagaaa
     6241 atggtaaaaa atatgtatac atagcaacaa aagacaatat agcaaagaaa cacttggttc
     6301 aaactggaat tgtaacagag gataaaatag aaattaaaag tggtgtaagt gataaagata
     6361 cagtgattat cggagggata agtcttattt ctgacggaac taagatattt cctgtagaaa
     6421 aggagaagta gactatgaat ttaacacaga cttccgttaa acgtcctctc acaatcataa
     6481 tggtcttttt agtattaata gtttttggtg gtattggata taaaaaaatg tcaataaatc
     6541 taatgcctga tatagaaata ccagttgtaa tggtcatgac tacttggaca ggtgctggtc
     6601 ctcaagacgt tgatgaacaa gttagtcaaa aagttgatga aagtttatct gccgtttcaa
     6661 atgtaaaatc tactatatca agttcacaag aaagtgtttc tatggtagtt gctcagtttg
     6721 aatttggaac taatttggat gaaataatga atgatgttag gtctaaagtt gatgcattac
     6781 agaccagcct cccagatgat gcagctaaac caacagtttt aaagctggat atgaatgcac
     6841 aggctatagg tcaacttgtc atatctggag gaaatgaaaa tagttcacaa gccttaagaa
     6901 aatacgctga agatgtaatt cagcctaaaa tagaatccat agatggtgtt acttcagcag
     6961 accttaaagg tggagaaaaa gcacaggtta atgtaatagc tgacccagct gttctttcaa
     7021 attatggagt tagtttatcc actataaaag gtgtcttatc ctctagtaat aaaacattcc
     7081 cttatggttc aattactcaa ggtgaggata aaatagtttt aagagcgatt gataaattag
     7141 aatccctaga tgatataaaa cagattcaaa ttcctgtaaa aggaggaaat acagttagat
     7201 tagataatct ttgtagtgta gattatggat atgcagacaa agatagtatt tatagataca
     7261 atggaaaaga cagtttggta atagatattt ctaaacaaca agatgccaac actgtaaaag
     7321 ttatgcagag tgcatacaaa tacgtagatg aattgaataa ggaaaatcct caatttaaaa
     7381 ttaaaattgc taatgataca agttcataca taagtgaatc aataaacagt gtaatgagca
     7441 acttgcttat aagtgctgtt atcgcattta ttgtaatatt tgctttccta aaaagtgtaa
     7501 gagcttcact agttgttgca gttgcaatac caatttctat aataggtgct atagcaattc
     7561 tctactttac tggtgaaagt ttaaatctta ttactgcaag ttcattggta atttcagttg
     7621 gtatggttgt tgataatgct acagttgtaa ttgaaaatat atttaaatat aggaaaagtg
     7681 gtagattgag tcttgatgat tgtgctattg agggaacaag aactgtaaca aatgcaatac
     7741 ttgcatcaac attgaccaca atagcaatat tcttacctat catgtttaca gaaggtatag
     7801 ctaaaataac ttttggggca ttaggtaaaa cactaatagc ttcactagca ttctccttta
     7861 taggtgcaat tacactcgtt cctagtgtat ttgcaaaatt aagcagagga aaaaatagtc
     7921 aaaagatgca agaaaaagat agtcctatat ttgataaggt aagtgaagca tataaaaaat
     7981 tattaagtgt atgtttgaga catcaacgct tagttgtaat atcaagttta cttatttttg
     8041 tagcttcctt atttggaatg ggatttatag gtatggactt tatgcctgca gcagacaagg
     8101 gtgaattatc tatttctata gaacttccaa aaggtcttag cctagaatcc aatgattact
     8161 atgtatctat gactgagaaa aaagtctcag atattccaga aattaaaact ttaataacaa
     8221 ctttgacatc agattcaaat aatagtaaag caagtatagc aattgaatta gaatctcaaa
     8281 aaaatagaaa aagaactact gatgaaatag aaaaagagat aattgataga gtggctactg
     8341 taccagattg taaaataaat gtaagtcaaa acagtagtgt tatgggtgga gctggaggag
     8401 cagacttcac attagaaatg aaaggtcctg atttagatgt attaaaaata ctgggaaaag
     8461 aagctgaaaa agaatttagc tctataaaag gatttagaaa tgttgaaact tctcttacag
     8521 atacaacaca agaagctcaa tttactatag ataaacataa ggctcaagaa tatggtgtaa
     8581 ataccgctga aattgcaggc atgttacgta cagcaataaa tggtgactcc gtaaccactg
     8641 ctactattga tgattacaaa gtggatgtaa acctaaaatt taaagcagat actattgata
     8701 gcttagaaga tataaagcaa ctaaaagtaa actctgcaac tggaaaagaa gttcctcttg
     8761 gtgccttttc agatataaaa atggcagatg gtctacaatc tatatctagg actgatggag
     8821 atttttctgt aaatataact gctacacttg acaatttaga tgctggtact gctacaaaac
     8881 tagcactaga aaaaacgaat aaaatggtaa ttcctaaaga ttactctata ggtgttggtg
     8941 gtgaaacaga aatgatgaat gaatctatgg caagtcttgg attttcaatg gtcatagcta
     9001 tagcattagt ttatatggta atggtagctc agtttgaatc atttagtaaa ccatttataa
     9061 tcatgttctg tgttccattt gcattcgttg gagttgtgct ttcgcttcta gttagtggtc
     9121 aaagtttaag tactgtcggt atgctaggta taatcttact tatcggtatt gttgttaata
     9181 atggtatagt tcttattgac tacatagagc aacttagaaa aacaaatcgt aacaatgatt
     9241 taattggttt ggtttcaatt ggttgtgcag caagattaag accagtctta atgactacag
     9301 caacaacaat tttaggtatg ttgccaacag cactatctct tggtgaaggt ggagaaacta
     9361 tgcaaccact agccatagtt ataatgggtg gtctaactgt atctacactt ataactttgg
     9421 tgcttatacc tactatatac ttaatatttg ataaatggga aaataagttt tcagacaaaa
     9481 tgcctaaaat atttggtaag ataacagata aaataggaag tataaaacgt aaaataataa
     9541 agaaaccaaa attgaaagct aaagtttaaa tagaatggag ggtgattgta ttgaataaaa
     9601 aaatgagcaa aatagttgca attagctgtg gtgttggtat aataactgcc aatttaatcc
     9661 cagtttacgc agataataat acttcttcta taaacaaagt tgataattct attttaagtg
     9721 actcaaataa aagaacatct gaaaaggaaa ttttgactgc tagagaagct gttagtgctg
     9781 cacttaaaaa tagtgaaaaa ttaaaaatga aatcagaaga aataaaaatg ttaaaagaaa
     9841 agttagaagt acaagatgag tttgatagtt ttactggaag tgacaactcg tttccatacg
     9901 accaaattgg acttcttaaa aatcaaagtg aacaggctaa aggctttatg gaggaccaaa
     9961 tagcaaatga tattactaat aaatttaacg atttagtttc aagagaaaat gagctagata
    10021 aaataaaaaa caatcttgaa ataaaaacta aagaaattaa agatatgaaa ttaaaaaaag
    10081 atttagggtt agtaacatct ttagaaactg aatcagctga acttgaactt caaacactac
    10141 aaaatactca aaaagctaaa cttcaagaac taaaaaacaa tcaagactat tttaagttat
    10201 taactaatat agatttaaat aattatcaat tggataaaga atatagattt gaatctttta
    10261 gagtaggtgg ctcagttgat tcatatatgg aaggtaaggt taatgaatat ttaaaatacg
    10321 accaattgat tttagaacgt acagaagaaa gctttaatga taaagatgag aacaaagcag
    10381 atttaccaga tagacctaac cttacaagac cagttgctcc tacaaaacca gagcaaggta
    10441 acctaactga tgatgaatac aaggttctta tggataagta tgaaaaagat tatgatagat
    10501 ataaaaaaga agttgaagca tacaatcttg aaagacagac ttatgctgct ggccttacta
    10561 cttatgcaaa ttatcttcaa caaaaattta atactgaaaa tggtctagta acattagaag
    10621 acagtaaaaa agctcttaaa aaagggttaa ttgatagcta tgctcaatta cttgctttag
    10681 aagatacaat tcagattact aaaaaacaac ttgatttatc agaaaaacaa ttaaaaaata
    10741 ctaaattaag atatgattta ggattaatta ctctaactga ttataaaaaa caagtggtaa
    10801 gtaatgaaga tgctaaaaat agctatgaca ctcttattgt gaattataac tcactaaaaa
    10861 atggtataga aaaaccttgg atacttaata ctggaaaata attctggtaa atagaaagat
    10921 tactttaaca tacgtttgat taaaaaatag ttatgcatac tcgtggcttt agtcatggaa
    10981 gattcagaag gcaaataata gaaatagatt agtaaaaata tatttactta taaaactgta
    11041 tacttttata gaggctatct caaaatagag ataagcctct tttttattgt ttaaattata
    11101 aaaatatatt tgattccata caaaaagagt gccttctgaa ctaaaaagtt cattttgaga
    11161 caccctcaaa agcttaactc taaatttaat tattttttat caaatgtttc atctatttta
    11221 catctcacca tgttaacaaa ttcagaaata ttatatcctc taagtttgca tccacattta
    11281 ggacaataat ccagtttcct agataccaaa gccctacaac taggttctgg acatcttatt
    11341 tttagactct catctgctac tttaaatcca catcgtgggc atatactacc tggacgtgat
    11401 gatacttcct ttccacattt aggacacttt gtcaacttta tcccctccag taaactcaat
    11461 ttaattgata gttgaaattt ctctctacaa taatatatgc aatataatta aatacgtttc
    11521 acttacaact atataagtta aatttttata ataaaaatcc cctatattca atttacaaat
    11581 acttaatttg atacattgaa caatagggga ttttattttt atttagttac atattttgca
    11641 gtctattatt atctaggatt ttttatagat gcttgagctg ctgcaagtct agcaattggc
    11701 actctaaatg gtgaacaaga tacatagtta agtcctacac tataacagaa ctctactgaa
    11761 gatggttctc ctccatgctc tccacatatt cctagtttta attctggttt aacttctcta
    11821 cctaatttag ctcccatctt aactaactta ccaacaccat tttggtctag tacttggaat
    11881 gggtcttttt ctaatatttc ttcatctaca tattgaccta agaatttacc agcatcatct
    11941 cttgaatatc caaatgccat ttgagttaag tcattagtac caaaactgaa gaagtctgct
    12001 tcttgtgcta tttcatcagc agttaaacaa gctctaggta tttctatcat agtacctaca
    12061 gtataaggaa cttctatacc ttcttctttt attatagcgt caacagtttc tactatcatt
    12121 tttcttatta tttttaactc atttacttct ccaactaatg gaaccattat ctctggagaa
    12181 actttaacac ctgctttgat tgcttctata gcaccttgta ttatagcttt agattgcatt
    12241 acacatattt caggataagt tattgcaagt ctacaacctc tgtgtccaag cattgggtta
    12301 aactcatcta aatcaactat tctcttctta atatcactta cttttattcc catagatttt
    12361 gaaagttctt ctatagtctc atcatcatga ggcaaaaatt catgtagtgg tgggtctagt
    12421 aatcttatat taactggttt tccatccata actttaaata actctacaaa atcctctctt
    12481 tgcattggta atattttttc aagagctttt tctctttgtt ctactgtatt agataatatc
    12541 atttctctaa ctgctggcaa tctagcttca tcaaagaaca tatgctcagt tctacataga
    12601 cctatacctt ctgcaccaaa ctctatagct gcacgagcat ctctaggatt atctgcatta
    12661 gttctaacca tcatacattt gtgtttgtct acccagttca ttaatttttc aaaatttcca
    12721 gttaaagata cttctgtttt cttaacatct cctaggtaca catttcctgt agaaccatct
    12781 atagaaatgt attctccttc ttttataact acatcatcaa gtgctcttat ttctttatta
    12841 aactcatcaa cttttatttc tccacatcca gcaacacaac atttacccat acctctagca
    12901 acaactgctg catgtgacgt cattccacct ctagctgtaa ggattccttc tgctttaacc
    12961 attccttcta tatcttctgg agatgtctct agtctaacta aaactacttt ttctcctttt
    13021 tcatttgctt ttactacatc atcagcattg aaataaactt taccacttgc tgcacctggt
    13081 gatgctggta aaccttttgc tataaccttg gcttctttta atgccttatc ttcaaatttt
    13141 ggatgtaata attggtctaa ttgattaggc tcaattctca tgatagcctc tttttcatca
    13201 attattccag cttcaactaa atcaaccgct acatttattg ctgctgctgc tgttctctta
    13261 ccatttctag tttgtagtat atataatctt tcattttcta tagtaaactc tatatcttgc
    13321 atatctttat aatgaccttc aagtatatga gtaattttta caaattcatc gaatacagct
    13381 ggcattacat ctgcaagagt tgatatattt tgaggagttc ttatacctgc aacaacgtct
    13441 tcaccttgtg cattcattaa aaattcacca aataatttat tttctcctgt agctggattt
    13501 ctagtaaatg caacacctgt accactagtt tctcccatat taccaaatac cattgattga
    13561 atatttactg ctgttcctaa gttatgagct atatcattca atttacggta tacgattgca
    13621 cgaggattat tccatgatct aaatacagcc tctattgcaa gcattaattg ctcttttggg
    13681 tcttgtggga aatcattttt tatttctttt ttataaattg ctttaaattc ttttactatt
    13741 tcttttaaat catcagttgt aagttctgta tcaactgtgt atccctttgc ttcttttact
    13801 ctgtctaata cattttcaaa tttatatttt ggaacttcca tagcaacatc agaaaacatt
    13861 tgtatgaatc ttctgtaact atcatatgca aatctttcat tttgtgtagc ctctgctaag
    13921 ccaacaacac tattgtcatt aagacctagg ttaagaattg tatccatcat tcctggcata
    13981 gagaatactg caccagaacg aactgatact aataatggat tcttattgca tcctaatgtt
    14041 ttatttaagt ctttttctaa agtagctaaa tgctcttcta tttcttttat tatctctgct
    14101 cttatacttt cattgttaac ataataatca ttacatgctt ctgttgttat agtgaatcca
    14161 ggagggacag gtaagcctat cttagtcatt tctgctaaat tggcaccttt acctcctagt
    14221 aaagatttca tgtctttgct tccttcacca aaactataaa catacttagt ttccattgtt
    14281 aaatccccca ttaatctttt tattttaatt tatatcattt ttattaatag acattattaa
    14341 atttctattt ctttgtatat ctttagacca ttttctttca ttatgtctaa aattatacct
    14401 gctgtttctt ctattgcctt atttgaaacg tttattactg ggcatccaat tcttttcatt
    14461 atctcttctg aataatctaa ctcctgaagt attctttcta aattagcgta gtttgcttta
    14521 ctggacaatc ctaaagcttt taatctttgg gttcttatct cattcagctt ttcaggtgaa
    14581 ttagtaagac ctattatttt tttagtatct atctcaaaca cttctttagg aattggtatt
    14641 tctggaacta atggaacatt agctactttt atatttttat tagctaaata catactcaaa
    14701 ggagttttag atgttcttga tattcccact aaaattatgt ctgcttgaag aacacctcta
    14761 gggtcttttc catcatcata tttcactgca aattctatag cttcaactct cttaaagtaa
    14821 gattcatcta attttctaat tataccagct tctcttttag gttttcttcc tgttctcttg
    14881 cttatttctc ttagtatatc tgtcattaaa tctatacaac taagaccttc tctttcgcaa
    14941 tatctttcaa caatactgca taattcttca tcaactaatg tatatacaat taaagcgttt
    15001 tcagcttttg cattctccaa agtttcaagt agtactcctt tattaatcat gtatggaaat
    15061 ctttttattt cataagtttc attcacataa aactgagaca tacatgcctt tgcaacttgt
    15121 tgtgcagtct cccctactga atctgatact gcatatataa ttaggttttt caccagcata
    15181 cctccttaat tgtcaactat ttctaagaat agcttagtta tgttggtttt tgatattctc
    15241 ccaacaactc tcatatgatt tttgtcttct cccttatatt caacaatcgg aatcgaatct
    15301 acttcatgtt ctattatttt tcttgaagct aataaaacac ttgctccttt agttaaagtg
    15361 actacatttg gagttcttgt cattatcata cctattggca ttttatttat atcagaacct
    15421 cctatagttg cttttagcaa gtcttttctg gaaacaactc cacataactc ttcattctca
    15481 tctataataa ctatactccc tacatcagat aaaaacatag tgactattac atcatatata
    15541 ctttcatcct tcttagctag tacaggcatg ctcattatat cctctactgt tttttctttt
    15601 acacttttac caattaaatt tatttcacta actcctgaat aaaaatatcc aacttttggt
    15661 cttgcgtcta atattcctgt cattgttaat atagctaaat cagatctaag tgtagcacga
    15721 gtcacattca agctggaggc aatactttca ctcgttattg gttcattttc cttaactata
    15781 tctataattt ttagttgtct ttcattaagc tgaataataa tcaccttctt tcattgtgct
    15841 atacttttta tcttttcgca tcttatatag tatattatat aactatatat accattctgt
    15901 aaacagattt tttattgaat acgatatatt ttttactaag ttctctcgct taaagatatt
    15961 tattatatat taatggggga tatttcttta attaatagaa ctatattagt attacagtta
    16021 atattataat acataattgg tcattttaat tcattttata ttaaaatttt taataatatt
    16081 atttcaatgt gaatataaaa ttgcagaact aattcaaaat aatttttacg actacaaata
    16141 acctataata tacttttaaa gaaagtatcc taacaagaat atataaaaat ttaaaagggt
    16201 tgatttaaaa ataaccatca acccttttta ctaatatcta tttatattac catcaaatct
    16261 tacccaaata ttttttcttg caattgtctt aaatgatata aagtagtatc tttttctatc
    16321 tctaccatat cgtaagttat aacttcacct tttttaatat cttttacaac tttagtcttt
    16381 ttacttatca aaccaatagg tacagcattc atagcctttg ctacatcgta tttttctata
    16441 gtaccatagc atgtatatgc tccatagcca tccataaact cacctttttt caaatctttc
    16501 ttagctatag tcataacttc tgctactagt ccagctttag gtactattgt agccttatgg
    16561 tctaagactg ccatagctgc tgataaagga gtctctaaac tacataaatg atatggtcta
    16621 tataatatgt aattaggtcc atcacccata cttaaatatt ttagttcatc attaactgct
    16681 tttaatttat gggctactac tacgaaaact ccaggtgcta taccatttac aaaatcaact
    16741 acaccataat tatctaatac tccaccttcg ctttttaatg catatttctt aggtacttca
    16801 ttaactgtag cttcaatacc atgaccacct cttacatcag gcacaaatcc agttgcatta
    16861 cacattaaag ccatttctac cattgtttta gttccttctt taaaagaagc tatcatatga
    16921 ggtgaagctc cttttctttc tgcttcttct ctaactgtat caggattaca atctaaatct
    16981 aatttattat ttttaccttt acctatcact ctaacttcaa accccattgc atctgcaaaa
    17041 tcaaataatt ccattactgc acctggctca tctcctgcag aacctgtaaa tattactcca
    17101 gcatcatctg ctaatttttt aagtattggt ccaacaacac aatcagtttc agcatttaac
    17161 ataactatat gttttttatt taaaatagag tttaatgcta tctctgcacc aattgcaata
    17221 cctccagttg catctataac tacatctatt gacttagttt tacaagccac aagaaaatca
    17281 ttacatgtca atacttttcc ttcttgtaat tttatttctg catccttttc atttgttaca
    17341 ttatctataa tttcttcatc tattcctgca tcagtaaaag ctttatgagc caactccaaa
    17401 tttatatcta caacaataga agcttcaata cctttcatat ttgccatttg gcttaccatt
    17461 ccatttccca tatgcccagc tcctataaga gctactttta tttttttacc ttcctcttcc
    17521 aattttgcaa gttttctgtt taaatctaac ataccaattc ctcctgttat aataatttta
    17581 ttttcaaatt aagaaatagt tattaaatct ccaatagtta tatctggatt tcctatttcc
    17641 acatgtattt ctcctggtaa atttacttca cattttccat caaatttgat tgtacaatgc
    17701 ccaatttctc taagagtctt ctgagcaata tcaccaactg aaactataca ataatctcta
    17761 ttccctatag taagtgtatc tcctatttca acatcacttt ttaacgttgc tattgaatga
    17821 attacagata tatcctttaa gtcgtcatca ttaatggttt catcatatat aataagacaa
    17881 ttgctttcat ccattaactc tttaactaaa ctgcctatcc cacttatctt aacttcatat
    17941 ttcatgacat tcctcctgat aatttatatt tatattgagt taattaaaag aaacatttat
    18001 tttttctttt ataaatttta tagcttgtac aatactctct tcatctttaa atttttgctc
    18061 tgcactatat atatctaatc cttctatttc ttcaatctct ttaaatttag ccattactgt
    18121 acgtcctttt aaaatttctc caaactttat aaacccatct gaatctgaag atataatggc
    18181 tatacttcct ttagaaaaat agcttttctt tttgcctgta gacactatat cacccttttt
    18241 tttagctcta tttaaagttt taaaatacaa tttattctgc ttcattataa atatagtatt
    18301 taaaatataa gcaacaaata aaatcactaa taatttcata tttatacacc ttccaaaaat
    18361 cacattattt ttaaatctgt ataacaacta taaatgctgt acaatagtta tataaggttt
    18421 ttaagtaaca cctttttaaa ttctaaaaaa gttctacatt tacttatctt attcgttaat
    18481 tttacatctt ggcctatctt taaaacttct tcataaattt ttactaataa gtctgagtct
    18541 atcttattct cacatggaaa cattgtcata agaattattt ttatacttct atttgcatat
    18601 tctattggtt cttctaaaat tccaactgcc attaaaaact tatctgattt ttcatttaca
    18661 gaatgaggaa ataataaacc tttatcaaat actgtgggag atttttcttc tctattgact
    18721 atattttttc taaacttatc atcaatgcat cctaaatcca taagatggtc catcattttt
    18781 tctaatgaag caagtatagt cttttcattt aatatcataa atttatcttc atctaacaaa
    18841 ttatttatta gtaaatttgt agaattagta gtgtcaatat tcccttctct taggcacaat
    18901 actgttttta attgttctct tagtttgttt tcatcaaata ttgtatttat ttttaatact
    18961 attgtattta tatttgatgt gtctaaatca atagttgtaa aaactatatc atattcatct
    19021 aagttaatat ttctcaaatt aaaacttgaa agagtatcaa ttttaggatt atttcctatc
    19081 agtttttcta ctcttatttt caatagtttt gctgttccta gacctgtact acatataatt
    19141 gctatttttt ttattgaata gaatctactg ctatttcttt ctaaatatcc actgaaatgg
    19201 attgctatat atccaatctc atcttctgaa acttctaagt tatacttttt ctctattact
    19261 tttcctgcta tttgtgctaa tttaaatgga agtatataat tttgcttaat ctcttctaac
    19321 aaaacatttt ttaactttat attaaataat agcctgttta aagtaaaact aagatgatat
    19381 cctagaccta ctcttaattc atcctcattt ataaaaatac ccatatcgac atatatttga
    19441 ttaaatatat tttccatcaa ctcatcaatg tttttactaa ttctactact atttaaagca
    19501 cattctgatg ctggtgcatt tccactaact aacggtatag ttaaataaat aatttcttca
    19561 tcatttaaag tacattcaaa cattcctgat attatatgtt ttatttccct agcaataaag
    19621 tattcatcat atgatttaag taaatcaaac tttacatcat aatcttttac tttcttttct
    19681 tcttttactc tatagactaa aactactatg tagttaataa cttctcttaa catttcatct
    19741 gtaacataaa aattattttt tttaaataaa gtcaatagtt tttcttctaa atttatgatt
    19801 tcttcttttt tgattccttt aaaatacttt gaattactga aatctttcct tgagttttta
    19861 tacaactgat ttaatataaa cagtctaata tcaagttcat ttccatttaa tttcatacct
    19921 gtattctgtt ttttaatcaa gtttaaatta tatgaagcca aaactttttc tacatactgg
    19981 aaatcattta ctaaagtagt tcttcctaca cacatctcat cagctaaact atcaagtgta
    20041 ataaatccat ctagttctat caagtaattt agtataaact ctactctatc ttcttttgaa
    20101 tttaatgaaa atacattttt tctattcata tcaataatct tattgagtat ctgctcatct
    20161 tttatttcta gtttatagcc ttgacccttt atatatttga ttactgctat atcattacct
    20221 aaatctttat tcaattgttt tatatacgtc cttacacttc tagttgtgac attcaattca
    20281 tttgctattg agttaatact cacaaactct tttttactac acataatcaa tatcttaaat
    20341 atcttgcggt caatagaagg aattttctca gttacactca ttttgtatct cctccttgat
    20401 ttcattctag tacaaatttg aaaccaaatt aatacataaa atttcacttt aataaaggaa
    20461 aatttagtat taatctaact cgataattac agctaattaa tagtataaaa gctagtcttt
    20521 cattaacaaa aaatcttgac tagcttaaat ttatatttac aattttatta attaattcca
    20581 agccttatat tgacattaaa tattatctca atgcaaatac tgcaactcca tatgcaataa
    20641 taacagctat tggaccagtt attaaacgag agtaaaataa agctggaaca ccatactcta
    20701 cagtttctgg ttctgcttct cccaaactta atcctactgg tacaaaatca catccagctt
    20761 gcccatttat tgcaaataaa gctggaagtg ctaaatatgc tggtattgca ccaagaccaa
    20821 tttgtgtacc tacaagagtc cctactactt gagctataac tgcaccaggt cctagtattg
    20881 gtgataagaa tggaagtgtg caaattactg atataatcaa taatccacca atattacctg
    20941 caagtggaga tataactctt gcaatccagt ctcccagtcc agatgctaaa ataattccca
    21001 taagcatact tacaaatgcc ataaatggta ttacgttatt cattaccatt tggatagtat
    21061 ctcgacctgc atcataaaat tttgatacta cttgccctgc cccttgacca atagaaagta
    21121 ttatgttctt tttagcatat tcaccttgtg cttttgactt aacctcatca taattatcag
    21181 gctttctaat agaactctta ttttcgcttt tattttcttg agatttttgt ggcatatctt
    21241 ccccatctac aacacttata cagttaggat taacatctga tacataatat tcttctgtta
    21301 taaattttgc taaaggacct gtttttccaa ctggatttac atttattgta ggtatttttt
    21361 tctttggata aactccacat ctagcagttc caccacaatc aataataact actgctactt
    21421 cttcatcaga aactccagtt ttaaatccat ctacaatttc acaacccagt agttctttta
    21481 tttttaatgt tacttcatgg ataccaccag cagtcattga cagtatctta tttctattcc
    21541 cttctttttt tatatacaaa ggacctcccc agcctgaagt tcccttttct atttttaata
    21601 ttttattctc ctccatttta tctacctcct aagccttatt tgttgctcta ttttttcttg
    21661 ttagaaaagc atacattttt tctgttacta tacctcttat aaaaattaca attagaccaa
    21721 ctataaaata ccacaatgcc aatgtagtag tttctttacc taaagtagtc aaacctgcag
    21781 atattcccaa ccatacaaaa agttctcctg catttgcatg tggaaaaaga cctgtaattg
    21841 gatgtacaaa tgaaactgct gcatcataaa atcctgcttt gtgctcttct tttaaaaatc
    21901 taccaaatgt atagcacata ggatttccca agaaaaatac tgatactaat ggaagtagaa
    21961 tatatcttgt aaaagagttc tttcctagct tagaagccca attattaact ctttcctcac
    22021 caattatctt aatcaaagag ttaatagcta ccattaatac aattagcgtt ggaagagtac
    22081 ctgaaacaag acctataaat tgttctcccc ctttctgaaa caaaccgata aaccactctg
    22141 cacctttact tagacctgta ataacgtttt ccataaaatt ataacctccc tataatatct
    22201 ttaaattttt aattttactg cttaccataa attttacaaa ttacatcttt tttctgtact
    22261 atatgctaat attgtaaatg tttttttatt aatatcattt taatataat
//
LOCUS       DAMJVI010000060        22228 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.062, whole genome shotgun sequence.
ACCESSION   DAMJVI010000060 DAMJVI010000000
VERSION     DAMJVI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.062"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            180..1241
                     /locus_tag="PC545_002324"
     CDS             180..1241
                     /locus_tag="PC545_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2457091.1"
                     /translation="MRDFLKNYKSSIILLACTIVGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKGTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKNLMVTQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     IGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIFMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAAMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1254..1451
                     /locus_tag="PC545_002325"
     CDS             1254..1451
                     /locus_tag="PC545_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HDF2457092.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1615..2319)
                     /locus_tag="PC545_002326"
     CDS             complement(1615..2319)
                     /locus_tag="PC545_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2457093.1"
                     /translation="MQQGSDYLKKFLEKHIDLLKKIPLFEGIKSDELEEMLECLGVVD
                     KTYTKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEVLFIKFKSVTGICSSACVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSHQFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFNKNKFEIFYFKK"
     gene            2484..3209
                     /locus_tag="PC545_002327"
     CDS             2484..3209
                     /locus_tag="PC545_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2457094.1"
                     /translation="MMRKIIKIDEEKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKAQKKTLACGCPGSQSKSINRE
                     VSNSMSISNDIVEIKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEVDYAEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3269..4861
                     /gene="hcp"
                     /locus_tag="PC545_002328"
                     /gene_synonym="priS"
     CDS             3269..4861
                     /gene="hcp"
                     /locus_tag="PC545_002328"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDF2457095.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGGTANLQDELTGVLIGL
                     SRATIGNKNRPTSETDKIMIEGLFTTITNVSFDDEAIKRQIEKTEVEKSKLVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYREDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGAIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDICGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5233..6138
                     /locus_tag="PC545_002329"
     CDS             5233..6138
                     /locus_tag="PC545_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2457096.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKYIFNKSYAEILRPKYENKNLKEVLLSIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6262..7215
                     /gene="msrA"
                     /locus_tag="PC545_002330"
     CDS             6262..7215
                     /gene="msrA"
                     /locus_tag="PC545_002330"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDF2457097.1"
                     /translation="MIKLATFAGGCFWCMVKPFDKYDGVIKVVSGYTGGHVENPTYQE
                     VCSGTTGHLEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESREHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTHIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPRENMEALGYGEYLKLFE"
     gene            7368..8498
                     /locus_tag="PC545_002331"
     CDS             7368..8498
                     /locus_tag="PC545_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457098.1"
                     /translation="MNLKDFNGKRLKSARIFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINIVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPNRKVMSDININDIESLAYKTREFWDLNSTPIVNMVSLLES
                     KGIIISGMNVDRKGATIFTQKQRISKESKYLISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPSKQFSEDEYACAFLLPKESFLKDLTHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LEKINYKKYNYLMNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLEEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDGKK"
     gene            complement(8615..9583)
                     /locus_tag="PC545_002332"
     CDS             complement(8615..9583)
                     /locus_tag="PC545_002332"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDF2457099.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFVVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRILSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPSTYSNLDLP
                     SIVEKNKKTGISIINGKDCTEFGIGTALVEIVKAILHNEKKVLPVSTLLEGQYNERNV
                     FAGVPCVIGKDGIEEIIEINMTEYEQNEFNKSCSVLREYIALSKTL"
     gene            9918..10106
                     /locus_tag="PC545_002333"
     CDS             9918..10106
                     /locus_tag="PC545_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457100.1"
                     /translation="MNYHTKEELVEALRVVSSSIINCGKGQKKFSEETSHHTRFKNII
                     EVMYISKSLIMDEISKRD"
     gene            10366..11565
                     /locus_tag="PC545_002334"
     CDS             10366..11565
                     /locus_tag="PC545_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HDF2457101.1"
                     /translation="MEKLYYTNQYIRDFTAEIIEIKEVENKYHVLLDKTAFFPGGGGQ
                     FGDLGKIESDDVINVYEDGKKIYHVLDKKPIKIHKVKCSIDWDRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGNEYSTVDIEGNLEEDKIREIELFANEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVIKIGDLDTNACCGVHPKSTGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDEVLKRDIYLTKICRYLSCSEEDAINGIVNLNSKVEEALIKKRRLE
                     EVVSTYQVKEMIEKANKVGNISVIKKIYDDEDLKYVNKIANKIAEYENSIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKVLAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11617..12120
                     /locus_tag="PC545_002335"
     CDS             11617..12120
                     /locus_tag="PC545_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457102.1"
                     /translation="MEQQKDYIELYENEELIEIREARMDDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENINAKATYESLNMYECDYNMY
                     EYEVIHS"
     gene            12227..12544
                     /locus_tag="PC545_002336"
     CDS             12227..12544
                     /locus_tag="PC545_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457103.1"
                     /translation="MGVLKGMFYVFLFLDLVSIFCFFFNKGKIASNKIVFNAIGVLTF
                     VLCFMLFSYYPNNNLIGKFIASLFFIFGVAGVMLKEKNFLYARLLLTVVIVFSTLRLF
                     VIQ"
     gene            12702..13661
                     /locus_tag="PC545_002337"
     CDS             12702..13661
                     /locus_tag="PC545_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2457104.1"
                     /translation="MIESKKSEKLLLSIIYIIFLVYILMIIRIILFRDVPIYAIFKGT
                     FRNVNLIPFYTIYQFIVDSNLDFMKATVNIIENIGIFIPMGIFLPIVCKNLNKKTIII
                     VIILVSLAFELTQYIFALGSSDIDDVILNSLGGIIGINIYINMNKLFPNDINRFKAII
                     GTSLVLGVIGLGVISKNYHNLLTFKFRPDRKISKILIEENREIIKDINKDTVDIVGTF
                     ESFKKGIITIKAGSNNKVKRPENLIDSDGNIRIYLNENTKLVSYIITKESKMDIVKYE
                     EFDVKNLDLLRRYDTINVWIDKENQPKDKHGIMASKLLIGLYE"
     gene            13776..14561
                     /locus_tag="PC545_002338"
     CDS             13776..14561
                     /locus_tag="PC545_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457105.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDKKFN
                     IKGRVCLKNAIYFGVMSVLIIRFIHPFIKYFVSIIPYRILISMAIITTLWTILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            14853..16172
                     /locus_tag="PC545_002339"
     CDS             14853..16172
                     /locus_tag="PC545_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2457106.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(16247..16675)
                     /locus_tag="PC545_002340"
     CDS             complement(16247..16675)
                     /locus_tag="PC545_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457107.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RKKDDKKKGNKKKNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAILII
                     TKLTDSTNKGNYSRNTVSDTHKNDIRNIVFNESNNFLICG"
     gene            complement(16766..18142)
                     /gene="hydG"
                     /locus_tag="PC545_002341"
     CDS             complement(16766..18142)
                     /gene="hydG"
                     /locus_tag="PC545_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HDF2457108.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIREEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLIAEEIGKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(18143..19228)
                     /gene="hydE"
                     /locus_tag="PC545_002342"
     CDS             complement(18143..19228)
                     /gene="hydE"
                     /locus_tag="PC545_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HDF2457109.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDAYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(19264..20472)
                     /gene="hydF"
                     /locus_tag="PC545_002343"
     CDS             complement(19264..20472)
                     /gene="hydF"
                     /locus_tag="PC545_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HDF2457110.1"
                     /translation="MSLNSTPQSVRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGELGELRIGKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLTKNKVKCPVVSVSSTDKIG
                     IENLKNEIIKVLPKDSTEFKLVSDLIEPNDLIVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDSGAISIVTKEDSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALENLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(20487..20738)
                     /locus_tag="PC545_002344"
     CDS             complement(20487..20738)
                     /locus_tag="PC545_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HDF2457111.1"
                     /translation="MKKVAVISAILEEPKECQFKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDTINSLTGKLGNIPHVSVKTSISKKEV"
     gene            complement(21119..22159)
                     /locus_tag="PC545_002345"
     CDS             complement(21119..22159)
                     /locus_tag="PC545_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDF2457112.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLEIKKDFENLGLDTI
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAIVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVITRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFEATESGFIKSRYIDNKSAVAMVLEICRYFKENNLTPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VDVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         8308 a         2681 c         3550 g         7689 t
ORIGIN      
        1 ctattaataa aacgttaaaa atacaaaaaa actatagaaa ttgacatata ttccagtatt
       61 tgctaaagta gctgttgggg aaaattacaa aaattattaa tgcgcattag ataatttact
      121 ttaatttttt tagcatttgg ggtaatttat aatataaagc attagaaagg aaaatataaa
      181 tgagggactt tttaaaaaat tataagtcat caattatact tttagcatgt actatagtag
      241 gggcgatagt aggattagta cttggtccaa aagcttcaat actaaaacca tttggagatt
      301 tattcataaa tgctctatta atgatagtag taccattagt ttttcttagt gtttcttcag
      361 gtgttgctag tataaaaaat atggaacgat ttggaaaaat aatgaagggt acagctatag
      421 tatttatggg aacaacactt gtaattgcat ttttaggtat tacatctgca ttattattta
      481 atccgtctaa aggattagat gtatcaagtg taaaaaattt aatggtgact caagatgcag
      541 aaactttaga atcaaaagga acgttattag accaagtagt atcaagtatt acagttaaaa
      601 attttggaga tttattttcc agtagtaatt tactacaatt agttatattt gcagttttaa
      661 ttggaacagc aatagctatg atgggagata ggggaaatac aataagaaag cttttatcag
      721 aaggaacgga agcaatatta aaagtagtaa gtatattcat gctttatgct cctatagggc
      781 taggctgtta tacagctaca ataattgggg aacttggaag taaaataatt caaggatatg
      841 taagggtgtt tgtaatttat tcactaacag cagccatgta tttcttatta ttttacagtc
      901 tgtatgcttt tttatctgca ggaaaacaag ggtttagaca attctggaga aatgctataa
      961 aaccaatagc aacggcacta gctacttctt caagttcagc atgtatacca tcaaatttag
     1021 aagctagtaa aaatatagga gttcctgatg atatatcaga aacagttata cctcttggaa
     1081 taaatataca taaagatggg tcagttttag cagtaataat aaaagtggca tttttatcag
     1141 gtattttagg tagtggaatg gcaaatccag caacgatagg tcaattattt ataactgtat
     1201 ttgttgttgg agtatttatg gctcctgtag caggtggctg acttattggt gagatgctcg
     1261 tggttagcat cttcggtttt ccagtggagg caattccact gttaacagtt ataactacta
     1321 taactgatat tcctaatact atattaaata caactggaaa tacagtatca tcaatgttag
     1381 tttcaagatt agtagaagga aaagattggc ttatagataa aacagcaatt actacaaaga
     1441 aaataagcta attgtacact taattaataa agttaatagg tatcttaatt ttaataaatt
     1501 agatacctat taacttttta agtaaaaata taaatttaag attcaaatat gatatttata
     1561 atttgaatca actaatatag tatatattga aataaatatg tttaaatatc tattttattt
     1621 tttaaaataa aagatttcaa atttattttt gttaaaagat aacaagcctt catctcgcat
     1681 cttgccaagc tctcttgaca tagaacttct gtcaacacat aaaaaatcag ccatttcatt
     1741 tcttgaaaag ggaattgtaa actggtggct acctgtttgt tctatctgtt ttgaaaaata
     1801 cataagcaat ttctcgcgag ttgttttacg agaaagtaat tctattttat tgtgaagtaa
     1861 aatgtttttt tcagctatta attgtagcat attttcaaca agcctattat gaaatacaca
     1921 tgcagatgaa caaataccag ttacactttt aaactttatg aataaaactt cgcaagaaga
     1981 tgttgtggtt actgtaacat tacttttatg tagtctagta catgcgaata cctctccaaa
     2041 taaatctcca ggcaagagtt cagctacaat attcctattc ccttcgatat cttcctttat
     2101 tatatgagca cttcctttaa gcataattcc aatagaagta acttcacttc caacagagaa
     2161 tattatacta tcttttgtgt aagtcttatc tacaacacct aaacattcca acatttcttc
     2221 aagttcatca cttttaattc cttcaaatag aggaattttt ttaagtaggt ctatatgctt
     2281 ttctaaaaat tttttcaaat aatcacttcc ttgttgcata tgcaaccgat aatttgtttt
     2341 cattgtacta tactaagttt ataaaagcaa ggggatataa aaatcctact acaagaaatt
     2401 tgaaataaag aaatataggt attaaaaata tatatataag aaatcaatac tttaaattaa
     2461 aaaaatttta tgaggtgata taaatgatga gaaaaataat taaaattgat gaggaaaagt
     2521 gtaatggttg tggactatgt gttgaagctt gtcatgaaga agctatagga atggtaaatg
     2581 gaaaagcaaa acttttaaga gatgactatt gtgatgggct gggagattgt ctgccaactt
     2641 gtccaacaaa tgcgatttca tttgagtata gagaagcagc agaatatgat gaagctgcag
     2701 taaaagcaaa tatggaagca aagaaagcac aaaagaaaac tttagcatgt ggatgtccag
     2761 gttctcagtc taagtcaata aatagagagg tttcaaatag tatgtctata agcaatgata
     2821 tagtagagat aaaaggttct caacttaatc aatggccagt acaaataaaa ttagtaccaa
     2881 ctaatgcatc atatttaaag aatgcaagtc ttttaatagc agcagactgt acagcgtatg
     2941 catatggaaa ttttcataat aaatttatga aaaataaggt tacattaatc ggatgcccta
     3001 aacttgatga agttgactat gctgaaaaac ttacagagat tttaaaagaa aatgatatta
     3061 aaaatatagt agtaacacgt atggaagttc cttgctgtgg tggtatagta aatgctgtta
     3121 agacagctct acaaaatagt ggaaaaatga taccatggca aatagttact atatctgtag
     3181 atggaaaaat tgtagatgga gaaatttaat taagtgaatt atagaaaaat ataataaatt
     3241 ttaataaata aaaagggagg aataaaaaat ggaaaaaatg ttttgttttc aatgtgaaca
     3301 aacagcagga ggtaaaggtt gtactataaa tggtgtctgt ggtaaaaagg ggggtacagc
     3361 taacttacaa gacgaactta caggagtgtt gattggtctt tcaagagcaa ctattggaaa
     3421 taagaataga ccaacaagtg aaacagataa aattatgata gaggggctat tcactactat
     3481 aacgaatgta agttttgatg atgaagctat aaaaagacaa attgaaaaaa ctgaggtaga
     3541 aaaaagtaag cttgtaccta gatgttcaga ttgttcaaca acttgtaata gaaatgataa
     3601 ctatgatatg aaggatttat ggaatgataa tgaggacata cgttcattaa aatcattaat
     3661 cttatttgga ttaagaggta tggctgccta tgcatatcat gcaatggtac ttggttatac
     3721 agacaaagac gtaaatgaat ttttctatga aggattaata gcagttggag gagacttgtc
     3781 tattgacgaa cttttaggtc tagttatgaa aactggagaa ataaacttaa aatgtatgga
     3841 attgcttgat agagcaaata ctgaaactta tggaacacca gaaccgactc aggtatctat
     3901 gaaaatcgag aaagggccat ttattgtagt tacaggacat gacctttatg atttacacaa
     3961 attattagaa caaacgaaag ataagggtat aaatatatat actcatggtg agttattacc
     4021 agcacatgca tatccaaaac taaaagaata taaacatcta aaaggtaatt ttggtacagc
     4081 atggcaaaac cagcaaaaag aatttgataa tattcctgct ccaatattat ttacaacaaa
     4141 ctgtttaatg cctgttaaag atagttatag agatagagta tttactacag aagtagttgc
     4201 ttatccaggt atggttcata ttgatgaaga taaagatttt tctagtgtaa tagaaaaagc
     4261 tcttgaactt ggaggatata gagaagatgt tcatatgact ggtattaatg gaggagatac
     4321 attaactact ggttttgcaa gaggtacagt ttcatctgta caagataaag tagtagatgc
     4381 tgtaaagagt ggagctataa gacacttttt attggttgga ggttgtgatg gtgcaaaacc
     4441 tggaagaaat tattacactg aatttgtaaa acaagctcct aaagacactg ttatattgac
     4501 acttgcatgt ggaaaatata gatttaatga tttagacctt ggggatatat gtggcttacc
     4561 tcgtttaatg gatatgggac aatgtaatga tgcttatagt gcaattaaag tggcagtttc
     4621 attggcaaat gcttttgaat gtggagtaaa tgatttgcct cttagcatgg tactgtcatg
     4681 gtatgagcag aaagctgttt gtatacttct tacactactt catttaggta tcaaaaatat
     4741 ctatataggg ccttctttgc cagcattctt atctaagaat gtattagata tattggttga
     4801 taaatttgca ttgacaccta tatctactcc tgaagaagat atgaagaaga ttttaggata
     4861 aataaacata tctatactta aagatattta tagttaaaat atttttaagt atgccacaat
     4921 taagataagg ggttaaactt aaaatttgat taaaaaatca attttgagat gaccctttat
     4981 ttattttttg taataattga aaaataaacc tgctttcagt atattctttt taagaatctt
     5041 tgtattaaat ttataatact tagatatata atatcttata tattttattt taagtattaa
     5101 aggtttgaat gtaacttaaa tattaaaaga ggaacattgt aatatgtaac ttgcataaaa
     5161 tatatatact atagtgaaat tattttgatt aaaatttaaa atataaggtg ttttatgtgg
     5221 gggtatatgt atatgaaaaa ttctaatttt aatatattgg catttgatgg tggtggatta
     5281 aaaggggcat tgagtatatc catacttgag aggatagtaa aagagtatcc aaatctatta
     5341 aacaatataa atatgtttgg aggtacatct acagggagtc taattgcact agggcttgcg
     5401 tatggtgtaa gccctaaaga gataaaagaa ctttactcaa ttgaaaattc aaagtatatt
     5461 tttaataaaa gttatgcaga aatactcaga ccaaagtatg aaaacaaaaa cttaaaagaa
     5521 gttttattaa gtatatttcc agaggaacta gaattaaaag atttaaataa attagtaatg
     5581 ataccaagtt tttatatagg aaatgaggaa aatgcatgga aaccagtgtt ttataacaat
     5641 atgccaaatt cttttacaaa gactagcaaa gttgtagatg tagcaatggc aagtagtgct
     5701 gctccggtat tttttccaac atataatcgt catgttgatg gaggaataat agcaactgac
     5761 ccgagtttag cgtgtataat tcatgccatt gacagtggat ttaaacttaa gaatacaaga
     5821 cttttttcaa tagggactgg atatgtatat aacagtataa aggcagatac tactgaatgg
     5881 ggagctattg actggataat taataaagaa cccgatttac caattatatc tattacctta
     5941 gaaggaaatt ctcaaatgag tcagttattt tctcaaaaac ttcttggaga taattactat
     6001 agattaaatc ctaagatgga aaaagatgta gctatggatg attgtgatgc attagaatat
     6061 ctaatgtctt taggagaaaa ttgtgatata aaagatgctt ttggatggat aaagaaaaag
     6121 agcttttata gaaattagat tcaatttgca attgtatttt tacacatgat ttcattgagt
     6181 aggtaaaaat aataaaatgc agattattaa gttacttctt agggaataaa taaagtataa
     6241 tttatttaag gagtgatgaa aatgataaaa cttgctactt ttgcaggtgg atgtttttgg
     6301 tgtatggtaa aaccttttga taagtatgat ggagttatta aagtagtttc tggatataca
     6361 ggtggtcatg ttgaaaaccc tacatatcaa gaggtatgct caggaactac tggtcattta
     6421 gaagcaattc aaataacata tgatgatgat ataataagtt atgatgaact tctaaatatc
     6481 ttctggaaac agattaatcc tacagatgat ggtggacaat ttggtgatag gggaactcaa
     6541 tataagactg ctatatttta tcatgatgaa gaacaaaaaa atatagcttt agagtcaaga
     6601 gaacatctgg aaaatagtaa aatatttgat agtaaaatag tgactgatat aattccagca
     6661 actaaattct acgaagcaga agaaaatcat caggattatt ataagaaaag accattaaac
     6721 tacaatatgt attataaagg gtctggaaga tataattttg taaaaagcca ttgggatgga
     6781 aacaatataa atagggaaga gttaaaaaag actttaactc atatacaatt tgaagtcact
     6841 caaaatgata tgacagaagt accttttgaa aatgaatatt atgacaataa caaagaggga
     6901 atttatgttg atattgtaag tggagaagtg ttattttctt caaaagataa atttgattct
     6961 ggatgtgggt ggccaagttt ttcaaagcca gttatagata aatctatcat tgagagaagt
     7021 gatttctcac aaggtatgta cagagttgaa gtaagaagtg ctaaaggaaa ttctcattta
     7081 ggtcatgtat ttgatgatgg tccaaaagaa cttggaggtt taagatattg tataaattct
     7141 gcttctctta aatttatacc aagagaaaat atggaagctt taggatatgg agaatattta
     7201 aaactttttg agtaattagg aatatttatt ataaacttat ctaaaaggtg aaaatataaa
     7261 aaaatatttt caccttttgt ttttttgtca ataatatgat aaattattaa ttgtaatctt
     7321 atttatacca atagattaag aatttaattt tgaaagagag aaaacaaatg aatttaaaag
     7381 actttaatgg aaagagatta aaatcagcta gaatttttag agcaaagacg atagaacagt
     7441 tgtctaaaga aactaaaata aataaaaaag atttaaaagc ttttgaggaa aataagtatg
     7501 taccaaatat agaaaataca ttaaaattat ctaatatatt aaattttcca aaagagtatt
     7561 tttataaaaa tgaaaatata aatatcgttg tagaggatag ccactttaac cctcaatcta
     7621 aactacctag agtagaagag atttcttata gagaaaaaat aattataata cataaaatat
     7681 attcatttat ggagaattat attaagtttc caaaagaaaa cttgccaaat agaaaagtaa
     7741 tgtcagatat taatataaat gatatagaat ctcttgcata taagactaga gagttttggg
     7801 atttaaatag tactccaata gtaaatatgg taagtttgtt agagtctaaa gggattataa
     7861 tatcaggtat gaatgtagat agaaaagggg caactatctt tactcaaaaa cagagaatta
     7921 gtaaagaatc taaatatcta atatctctgg gtaatgacaa aaagtcagca tcaattagaa
     7981 attatacttt ggcatgtgag cttggatata taatatcaag tgaattaaga ataccaagta
     8041 agcaattttc agaagatgag tatgcctgtg catttttatt gccaaaagag agttttttaa
     8101 aagatttgac tcatccagaa gatttagatt attatgtaga actaaagaaa aaatggattg
     8161 tacctatatc tgctatgatt tttagagcat ataatctaga aaagataaat tacaaaaaat
     8221 acaattatct tatgaatgaa atggataaaa aaggctggtt gatagaagaa ccactagaca
     8281 aaatgaaagg ttcaagccca acgtatttaa aaagagcagt tgaattattg atagaaaata
     8341 aaataatgag tgtaaatagt atagtatcaa gtttagagga atttggaata aatctttatc
     8401 cagaagactt agaattattg atgggattga aaaaaggatt gttatctcaa gaggtaaata
     8461 aaaaaagtaa agttataaaa tttgatggta aaaaataggt ttatatttta gtattaaaat
     8521 taaaagcagg tacaaatgat ttaaacatta acttagttaa aagtcttttc aataagtcat
     8581 ttacctgctt ttagtgtaag aaagaatatt gaatctaaag tgtttttgaa agtgcaatat
     8641 attcccttaa aacagaacaa gatttattaa attcgttttg ctcatattca gtcatattga
     8701 tttcaataat ttcttcaata ccatcttttc ctataacaca tggaacacct gcaaatacat
     8761 ttctttcgtt atattgacct tctagtaatg tagaaactgg aagaactttt ttctcattgt
     8821 gtagtatagc ttttacaatt tctactaaag cagtacctat accaaattct gtacaatctt
     8881 taccatttat tatacttatt cctgtttttt tatttttttc aactattgat ggtaagtcta
     8941 aattgctata agtacttggg ttgtctttta tcatgtctaa gattggttta ccaccaattg
     9001 aaacatgaga ccaaggaacc atttgagaat ctccatgttc acccatagaa taagcttgaa
     9061 ttgattgttg agcgattcca gtttcttcag ataaaatcct tctaagtctt gttgaatcta
     9121 aagctgttcc agttccaatg actttgtttt taggaaagcc agatttttcc caaatataat
     9181 ttgtaactac atcaacagga ttagatatga ctacaaatat accatcaaag ccagaatcaa
     9241 ctatgggttt tactatagat tttatcattc caatagttgg tcttagtaaa tctaatctag
     9301 ttgtgttttg tctacgtaaa ggcccttctg aagagtctgc aacacttatc actactatgt
     9361 cagaatcttt acagtcagaa aaagttcctt tttctatatg tactttatgt ggcagatatg
     9421 aaactgcatc agctaaatct aaagcttgag cttttgattt agattcatct atatcaatca
     9481 taaagagttc atcacaaact ccttgagtta ttaaactaaa tccacaatga gaaccaacat
     9541 gccctgaacc tacaatagat atttttcttg gctttattgc cattttaaat cactccccct
     9601 aaaataacga tacattgaaa attatagatg ttattaaaag ctgtttttgg atattttaat
     9661 attctattat ttcagaaaat ttatttaata tgattttata ctaaaatttt taaaaaagca
     9721 atgttaatat actctctatt agtgaatatt aaagtattat atgtatatta ataattatta
     9781 aaatatgtaa tgtcactgat agttagaata tttaagtgct aaaagatgtg tataaataat
     9841 aatgatgtca gtttataatg atattaaatt gagatatact gtttctctaa attaaaaaat
     9901 aaaggagtaa atgtgttatg aattatcata caaaagaaga attggtggaa gcactgagag
     9961 tagtatcttc aagtattatt aattgtggaa aaggacagaa gaaattttct gaggagacat
    10021 ctcatcatac acgttttaag aatattatag aagtaatgta tatttcaaaa tcattaataa
    10081 tggatgaaat tagtaaaaga gattgaagct tattttgcaa tttaacatgt tttaatatac
    10141 attttattat ataagaaaat aagtaaacat acatgttata aaatattgga ataatataca
    10201 attataaatt tgttaacact aatatctata tgttaaaata tagtagaaga tgttagtaaa
    10261 taataggatg agttctattt taatacaatt aaattaagct tagtttgaaa tttgtatcta
    10321 aaaagtaaag taatctagaa ttaagaaaat ggagatagaa ttataatgga aaagttatat
    10381 tatacaaatc agtatataag agattttaca gcagaaataa ttgaaataaa agaagtagaa
    10441 aataagtatc atgttttatt agataagaca gctttttttc ctggtggagg aggtcagttt
    10501 ggagacctgg gaaaaataga gagtgatgat gtaataaatg tgtatgaaga tggaaaaaaa
    10561 atataccatg tattagataa aaaacctata aagatacata aggtaaagtg tagtattgat
    10621 tgggatagaa gagaagatgg aatgcatcaa cattttggac aacatgtatt gtctggatgt
    10681 ttttttaaaa cttttaatgc aaatacgaca ggttttcatt tgggaaatga gtatagtaca
    10741 gtagatatag aaggtaattt agaagaggat aaaattagag agatagagtt gtttgccaat
    10801 gaaattattc gtcaaaatat acaagtagag acattacttc caacgaaaaa agaactaaaa
    10861 aagatttggt taagacgtga tttgccaaat actgatgaag agataagagt aataaaaata
    10921 ggtgatttag atacgaatgc atgttgtgga gtgcatccaa aatcaactgg tgaattgaga
    10981 atgataaaaa taaagaaatg ggaaaaacat aagtcaagta caagaataga gtttctagca
    11041 ggtaaaaggg cagtggatga agtattgaag agggatatat atttgactaa aatatgtaga
    11101 tatttaagct gtagtgagga agatgctata aatggtattg taaatctaaa tagtaaagtg
    11161 gaagaggctt taattaaaaa aagaaggctt gaagaagtag tatctacata tcaggtcaaa
    11221 gaaatgattg aaaaagcaaa taaggttggc aatatttcgg tcattaaaaa aatttatgat
    11281 gatgaagatt tgaaatatgt aaataagata gcaaataaaa ttgcagaata tgaaaatagt
    11341 attgcattat tagctgttaa atttaatgat aaaataaatt tagtttttgc atgttctaaa
    11401 aatttaaaag atattaatat gggagtttta ttaaaggatg caataagtct tatagatggc
    11461 aagggtggtg gaagtaaagt attagctcaa ggaggaggta agaataatgg aaatttagag
    11521 gctacacttg attatgcatt tatgaagata gaaaaaactc tataaatata ttatataaaa
    11581 aaccttagta gataaaatag atatggagga ataagtatgg agcaacaaaa ggactacata
    11641 gagttatatg aaaatgaaga actaatagag ataagagaag cacgtatgga tgatttggat
    11701 acaatagcaa aatttaatta taatttggct aaagaaactg aaggaaaaga acttgatatg
    11761 gatgttttaa ctaaaggtgt aaaagcattg ttattagatg agagaaaagg aaaatatcat
    11821 gtatatacag tatttgataa agttgttgca cagattatgt atacatatga gtggagtgat
    11881 tggagaaatg gaaatttcct ttggattcag agtgtatatg tagataagga atatagaaga
    11941 aaaggtatct ttaattattt atttaattat ataaaaaata tatgtgataa agatgaaaat
    12001 atagttggaa tgagattata tgtagaaaaa gaaaacataa atgcaaaagc aacatatgag
    12061 tctttaaata tgtatgagtg tgattataat atgtatgaat acgaagtaat acattcgtaa
    12121 tataaatttg taatactgtt atactgatgt tataaagtat tgattatatt gacgattata
    12181 tgaaataaac tggatattat aattagaatt tttagggggt ataaaaatgg gcgttttaaa
    12241 aggaatgttt tatgtatttt tatttttaga cttagtgtca atattctgtt tctttttcaa
    12301 taaaggaaaa atagcatcta ataaaattgt gtttaatgca ataggagtac ttacatttgt
    12361 actttgtttt atgcttttta gttattatcc taataataat cttattggaa aatttattgc
    12421 ttctctattt tttatatttg gagttgcagg agttatgttg aaagaaaaaa attttttata
    12481 tgctagactt cttttaactg tagttatagt tttttctaca ttaagattgt tcgttattca
    12541 ataaaatgta tttgtatgaa aatttattaa agtctacaca tttaagtgta ggcttttttg
    12601 attttttgtt tgaaaattaa ggaaatttta aggaaatttt aaaatcgtct taaatattta
    12661 ttgatatact taaaatatta atagcgaaag gagacttatc agtgatagaa agtaaaaagt
    12721 cagaaaaatt gttactgtca attatataca taatattttt ggtatatatt ttaatgatta
    12781 taagaataat tttatttaga gatgtaccaa tttacgctat tttcaaggga acgtttagga
    12841 atgtaaattt aattccattt tatacaatat atcaatttat agtagatagt aatcttgact
    12901 ttatgaaagc tactgtaaat attatagaaa atataggtat atttattcct atgggaattt
    12961 ttttacctat agtatgtaaa aatctaaata aaaaaactat aattatagta attatacttg
    13021 ttagcctagc atttgaatta acacaatata tttttgctct tggaagtagt gatatagatg
    13081 atgttatttt aaattcatta ggtggcataa ttggaattaa tatttacata aacatgaata
    13141 aattatttcc caatgatatt aacagattta aggctataat aggtactagt ttagttttag
    13201 gagttatagg attaggagtt atttctaaaa attatcataa cttactaact tttaaattta
    13261 gacctgatag aaaaatatca aaaatattaa ttgaagaaaa tagggaaata attaaagata
    13321 taaataaaga tacagtagat atagttggga catttgagag ctttaaaaaa ggaataataa
    13381 ctattaaagc aggttctaat aacaaagtaa agagaccaga gaatttaatt gattctgatg
    13441 gaaatataag aatttattta aatgaaaata caaaattagt atcttatata attaccaaag
    13501 aatcaaaaat ggatatagtt aaatatgagg aatttgatgt gaagaattta gatttattaa
    13561 ggagatatga tactattaat gtttggattg ataaagaaaa tcagccaaag gataaacatg
    13621 gtattatggc tagtaaatta ttaattggat tgtatgaata aaatattaaa caagaataaa
    13681 agaataacaa atataaaaaa cgaaaaaact atttctataa ttatttttac gttgtgcata
    13741 aaataagaac attagtgaag gagtagagat aaattatgga gataataaaa tatatcttgt
    13801 acttttttat atattcattt ttaggatgga cagtagaaag tataggatgt tctatagcaa
    13861 gcaaaagaat aatcaacaga ggttttttaa atggtccaat atgccctgtt tatggttttg
    13921 gagcggtaat tgtgatatca ctattgggaa gatttaataa tgtagtaata gtatttctat
    13981 taggaatgat tttaactacg atactagaat attttacagg atttatttta gaaaccttat
    14041 ttcatgctaa atggtgggat tattctgaca aaaaattcaa tataaagggt agagtatgtt
    14101 taaaaaatgc aatatacttt ggtgtaatgt ctgttttaat aataagattt atacatccat
    14161 ttataaaata ttttgtaagt ataattccat atagaatatt aatttcaatg gctataatta
    14221 caacactttg gactatactt gatttgattg ttactattat aacacttaaa aaattagata
    14281 taaagttaaa cttattagac gatataatta cagaccttaa tgatataaat gtgaaattgg
    14341 ataaatttga tagaggagaa atacaagctt tatttaaaac aataaataac gacgaattag
    14401 aagttagaga aaaaataaac aaaataaata gtaagttgga ccgtatagag tctaatatta
    14461 ttttacaaaa aagagttata aaagcttttc cacatataaa acataaaaaa caacaagagc
    14521 aactagaaca ttttaaaaaa attattaatg agaaaaggta attatacaat ttaatgaaga
    14581 cgaatagaat aatggtattt ataagatgat tttcagagga ctctataaaa atagaataga
    14641 gtcctttatt ttttgaaaaa taatgtttat ttgataaaaa taaaatatat tgtttctgaa
    14701 taacaaatat tttgaaaatt ataaaaacat atattgacaa tttgtgtcaa aaatactaac
    14761 attaagtata taaagaattt tatatgagaa tatctttata aatcgcaata attttaaaac
    14821 ataaatttat ttaaagaggg gatgatttgc caatgagcga gataactagt caaatgataa
    14881 gtacagaaga aaaaaaatat gttgctaaaa ctcaaaagat accttattat ccagtagcat
    14941 ttaaatcagg tgatggtgca atgctatatg attatgaagg taatgaatat gtagattttt
    15001 tagcaagtgc aggctctgca aatgtaggtc atggtaataa ggaaatctca caagcagtta
    15061 aagaacaaat ggatgatata acacaatata cacttgcata tttccatagt gatcctcctg
    15121 taaaattagc tgagaagctt gtggaaatag cccctggaga taatgataaa aaagtactat
    15181 acagtgcaac tggttctgct tgtatagatg ctgctataaa attagcaaga gggtatactg
    15241 gaagaacaaa gataatatct atgtgtgaat cttatcatgg aagtacttat ggagctatat
    15301 ctatatctgc tttaagtaca aatatgagaa gaaaaatggg tccattgtta ccagaagttt
    15361 atcacttcca ttatcctgat aaaaatagaa cagctaaaga atgtttagat gagatagaat
    15421 atgcttttgc acactatcta ccagcagaag aggtagctgc aatatttata gagccaattg
    15481 caggagatgc aggaataata gtgcctccag tagagtgggt acaaggttta agtaaaatat
    15541 gtaaagaaaa tggaatatta cttgtaagtg atgaaataca acaaggtatg ggaagaacag
    15601 gtaagtggtt tggtatagaa aattttggtg ttgaagcaga ccttatagtt cttggaaaat
    15661 ctgttggagg tggattacct ctaggagcag ttgttggaag aacagaaata atgcaaagtc
    15721 ttgatgcacc agcacattta tttactttag ctggaaacac aacagtatgt gtggctgctt
    15781 taaaatctat agagataatt gaaaaagaaa acttacttca aaagagtata gaaatgggag
    15841 attatattaa agcaggattt gaaaaactaa aagaaaaata cgatataatt ggagaaataa
    15901 gaggcatagg attatctata ggagttgata tagttaaagg aaaaggttct aatgaaaaac
    15961 atccagatgc tacagctaag atatgttata gatgtataca aactggatta ataatgatat
    16021 tcttaggaca atctacttta agagtgcaac cacctctagt aataactaag gagcaagtag
    16081 ataaagcaat gaatatcata gattctgcaa tagatgatta tttaaatggt agaattggtg
    16141 atgaagtata tgaagtaact caaggttggt aaaataaata ttaaatgaaa ataggagtta
    16201 tctcaatttt agaaaattat ttctgaaaag agataactct tttaagttac ccacaaataa
    16261 gaaaattgtt tgattcatta aagactatat ttcggatgtc gtttttgtga gtatcagaga
    16321 cagtattacg agaataatta cctttattcg tactatctgt taatttagtt ataattaaga
    16381 tagctaaaag aataagaatt actgtgtata aagcaataac atatggtttt cttttatatt
    16441 tgtatttgtt attgaaaaaa gaatggccat aattattttt atttacttta gatgtatttg
    16501 tattcttttt cttgttacct ttctttttat catccttttt tctattttta gggtaaggaa
    16561 tgactttgtg tgtattatca agtttgatat ttttattttt ttgaatattg ttttccaggc
    16621 tagctttttt ttctccaaat tgaatagttt tattggtatt gttgttatct ttcaatgtta
    16681 atctccctcc cttaaacttt ccctttagat aatattacta ctaacctatg gaatatacaa
    16741 taaataatat ataagtaata aaattttata ggtataaatc tctttctcca cgctctaagg
    16801 cagtcatatt gttagatact atgtttctta tatcttctct ttctatctta cctatttctt
    16861 ctgcgataag ttttttaccc ataattttaa gttcttcatc tccataatcg attaaaaatt
    16921 catttaatgt agtaagagca tttggtgtac atacattatg gatttgtcga cttttagcta
    16981 agctcatgaa tctatcgcca gttctacctt tacgataaca agctgtacaa taactaggta
    17041 tatatccatc agtaatcagt tctttaagca cctcaacagg gcttctatgg tctccaactt
    17101 cgaattgttc tgtattgtca ccatcttcat atgctttata tcctccaaca cctgttagag
    17161 aacctgcact aatttgtgat acaccatatt ttagtaactc atttctcatt tcagcagttt
    17221 ctctagtgga cattatgatt ccagtaaaag gaacagctaa tctagttata gctactattt
    17281 ttttgaacat atcgtcagaa actaagtgag gaaaatcttc taagctcata ccttcagctt
    17341 ttttcaatct agggaaagat atagtatgga aacctacacc aaatttttcc tctaagtgtt
    17401 cgttgtgcat cataagtcct agaacttcaa atttagggtc agataaacca aataaaactc
    17461 ctgcaccaac atcatcaaca ccagcttcca tagctctatc aaaagctgtt aaatggtaat
    17521 aatagtcatt ttttatagat tgaccatgca tttttataaa tgttggtttg tgataagttt
    17581 cttggaataa tatataagtt cctattcctt tttcttttag taatttatac tcatctacac
    17641 ttgtagctgc tatgttaacg tttacccttc ttatatttcc gttttcatta tatgttgaat
    17701 aaattgcatc taagcaatct aagatgtaat ttatatcaca atttttaggg tcttcaccag
    17761 cttctagagc aagtctttta tgacccattt tttctaatat tttaacctct tcccttatct
    17821 cttccatggt aagttttttt cttttaaatt tgttgcattt actaaaaccg caataaacac
    17881 attcatttac acagtagttt gatacataaa gaggggcaaa taaaaccact ctattaccat
    17941 atatttcatt ttttatttgc ccagctatac taaataatct atctagttgt tttttatctt
    18001 ctacttcaag aagtgtagct atatctttat aactaagttt ttcccttcta tcagccttat
    18061 caagtacctt ttcaatatca tcagaagttg aattttttgc atcttctaat aaagaattaa
    18121 taagttcatg gtttatgaac atttacaaac gcctccattc aatattatca ccgcgagaat
    18181 agtcaacttc aagtcctaat gacttgatgt tttcttctaa actttggtga tattcacaag
    18241 cttcttgacc agtaaaggct ttgttatcgt aaagagaata ttgttttcta aattcttgag
    18301 gagaaaggtt tggcataatt acattgcaac cagctaaaag acctgcattt ctaccactag
    18361 ggtttataga tgccaaggct gttgtagcgg gtaataaaac ttttggaagt agaagtcttg
    18421 taattgaaag cataagtaat gtcttctcta aatctccatg tttataatcc ttaaaaattg
    18481 tatcatggtg tggaatgaaa ggtcctattc caaccatatg aggatttaac tcttttaggt
    18541 ataataaatc acgaacaagg tcttcgttgt tctgaaatgg tgattcaacc ataaatccag
    18601 cacctacctg ataaccaatt tctttaaggt tttttaggca ttcttttcta gttctaagct
    18661 caatgtttgg cggatgtaat tttttataat gactgtctgt agcagtttca tgtcttaaaa
    18721 gatatctatc tgcaccacat aaaaagtatt ttttatacga atcataactc ttttctccta
    18781 gtgatagagt tatggcacaa tcctcatatt tattttttat acttgaaaca atttcacata
    18841 ttttatcatc agtaaaatat gcatcttcac caccttgcaa aacgaatgtt ctgtatccta
    18901 ttttataacc tatatcacaa cattcaagta tttcatcaag tgaaagcctg tatcttatga
    18961 tatttttatt agaagattgt attccacaat agtagcaatt atttttacaa tagtttgtaa
    19021 gctctataag tcctctcaag tagactctat ttccataatg tttatttctc atgtcgctag
    19081 cttgtttata aagatagttt cttaaatttt gttcgaagtt atattcctca ttgtcgatat
    19141 tttctattaa atatagtagt tcatcaaaac ttaatgaatt aatgctgtaa agtttatcta
    19201 taaagtattt aatcttattt ttagtcatat tcaaatatat aattattaag tatagttaaa
    19261 tttttaataa ttgaaaagtt caagtgatct ttccaaaata ccttttacat atgctattaa
    19321 gataccatag tttacaatag gtacattgaa agattttgcc ttttctattc tagataacat
    19381 tccagctcta ttcatcatac aagctccaca gtgaactaca agggcatatt tatttatatc
    19441 ttcagtgaag gatactccag aactaaattc aaaagtaatg tttttgccag ttttctttct
    19501 aatcatatta ggaattttta ctgttccaat atcatcagtt tgtctatgat gggtacatcc
    19561 ttcggccatc aagattttat caccatcttt taaattttct aaagcataag cgccattaat
    19621 caattcttta aggtctccct tttgtctagc aaacaatata gaaaaagatg taagaggaat
    19681 atcttttggt gtatctttat ctacttgtgg aaatacttga gagtcagtaa taacaagttt
    19741 tggttttttg cctaaattag atagagtttc ttttaaacta tcttcttttg taactataga
    19801 aattgcacca ctatccaaaa tatctcttat aacttgctgt tgaggaagta taagtctacc
    19861 ttttggagct gctttatcta ttggaacaac taaaacaatt aaatcatttg gttcaattaa
    19921 atctgatacc aatttgaact cagtgctatc cttaggcaaa acttttatta tttcattttt
    19981 taaattttca attccaattt tatctgttga tgagacagaa acaactggac atttaacttt
    20041 atttttagtt aagtttaaaa tttctgattg attcttaata gtatcaattt tgttgagaat
    20101 tagaatatga gggatattct tatcattaaa cttttcaatt agtgacaaat cttcttgtga
    20161 tataccaatc tggcaatcca ctactagtaa agctatgtct gtcttttcta aaacatctag
    20221 tgatttacct attctaagtt caccaagttc accaacatca tcaagaccag cagtatctat
    20281 aagaacacaa ggtcctatag gaaggatttc cattggtcta aatactgggt ctgtagtagt
    20341 tccagcaata tctgagacta ttgcagcact ttgatttgtt atagcattta ttatgctaga
    20401 tttacctgca tttcttttac caaagagacc tatatgtact cttaccgatt gtggagtaga
    20461 atttagactc atttgtactc accaccttaa acttcttttt ttgatatact agtttttaca
    20521 gatacatgag gtatgttgcc caatttacca gtcaaactat taatagtatc catttcgcca
    20581 acaactgcta tacatacgat tgagacacct tcttcttcgc aaggaatgcc cattcttcct
    20641 ttaataatac ctttaaaacc tgatacaacc tcattgaatt taaattgaca ttcttttggc
    20701 tcttccaaaa tagcacttat aactgcaacc tttttcataa atatcctcct aactcagaaa
    20761 atatgtctta aacaaatcca aaatcaaaaa acttttcttt gtaaagaaaa gtttctacaa
    20821 aagaaaagtt attaattaca caagtataat gtgctatatt aaactatttc cttacagatt
    20881 agataactct tttctggtag ggttagcttg taaaaattta atttaacaac aagtaactga
    20941 agaaaatttt tatacaattt atataaatta taataaaaat taactaaaga aaattgaagt
    21001 tacttatata atagtagtaa attgaaatta agtctataga aaaaccaatt tttgtataaa
    21061 aataaaaatt aaagttaatt atttgttaag aattgacttt ggtaaaatat ataattaatt
    21121 attctttaag catataattt ataagtaatt ttagtgtatt ttcaatagct tctatatgtg
    21181 ttctttcata atggtgacta ttgtttgttc caggaccaac acatgctatt tgaacatctt
    21241 ctccccaacg aatagcttct gaagcatctg aaccatagaa agtaaataca tcgacagcgt
    21301 agtctaaatt attgtcttct gcaacatcaa caagcttgtt tcttaaatta aaatcatata
    21361 atgataaatt atcttttgca actatagtta caccatgttc actagatgtt tgacttggtc
    21421 cagtaggtgc tatatctatt gctacaaatt cttttgtttt ttcaggtaaa catttagaaa
    21481 caccatgtcc aatttcttca taattactta tataaaagtt agtagtatgc ttaggtgtta
    21541 aattgttttc tttaaaatat ctacatattt caagaaccat tgctacagct gatttattat
    21601 ctatatatct agattttata aaaccagatt ctgtagcctc aaatctatgg tctaggcata
    21661 caaaatcccc aacacttatt cctaaatcta atacatcttg ctttgtaaat actttttcat
    21721 caagtctaat aatcattgtt tcttcatttc tttcagtggc agcttttgtt tgaccatata
    21781 tatgagtaga agcatactta aatacaacac ttcctctgat ttttttacct tttctagtta
    21841 taacagtaca gttttctccc tctatagctg cccaacatcc accaccaact ttatggtatt
    21901 taagagtacc atcagatgat atttctttta ctatagctcc aagtgtatcc atatgtgcag
    21961 atattgttat ttgatttgaa tcatcttcac caggaagagt agctataagt gcaccttttt
    22021 ttgtaagaat agtgtcaaga cctaaatttt caaaatcttt tttgatttct aaaactgcat
    22081 tttcagtata tccagctggg cttggaatgt ccaaatattt tttcattaaa tttatagtat
    22141 tagttaaatt taaattcata ttgtttcctc ctaaataaat atatttatac aaattataac
    22201 ataaatacaa atgttctgaa aactaaaa
//
LOCUS       DAMJVI010000061        22146 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.063, whole genome shotgun sequence.
ACCESSION   DAMJVI010000061 DAMJVI010000000
VERSION     DAMJVI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22146
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.063"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            444..1514
                     /locus_tag="PC545_002346"
     CDS             444..1514
                     /locus_tag="PC545_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457113.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGRLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGSPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVVTKEVLGNETIYCLSVLNREIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDVKVCKSMYSKIKGDIYEKAI"
     gene            1498..2367
                     /locus_tag="PC545_002347"
     CDS             1498..2367
                     /locus_tag="PC545_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDF2457114.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            2411..3271
                     /locus_tag="PC545_002348"
     CDS             2411..3271
                     /locus_tag="PC545_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDF2457115.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIVQFLVMVP
                     AAYAFSKIKFKGEKMLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQISDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            3302..4606
                     /locus_tag="PC545_002349"
     CDS             3302..4606
                     /locus_tag="PC545_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2457116.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNSNDANASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQERFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            4669..5931
                     /locus_tag="PC545_002350"
     CDS             4669..5931
                     /locus_tag="PC545_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDF2457117.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKNLPNFKSILENCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKAKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNRHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNDEVDKALKMNDKWLG
                     DIIQATKDAGTYKDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKDFDAYV
                     NSSGISAQIIVNNLDRLTELRELLEEFKEELFIENIFTKEEASNLGLEGDFEMVIEGL
                     EGISFGNDFEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKGGVVIEEGD
                     LRAHAATILKMFNLKLDGVEKEAFDFIM"
     gene            6118..8088
                     /gene="uvrB"
                     /locus_tag="PC545_002351"
     CDS             6118..8088
                     /gene="uvrB"
                     /locus_tag="PC545_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDF2457118.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIVEIENELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNIIPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            8107..10932
                     /gene="uvrA"
                     /locus_tag="PC545_002352"
     CDS             8107..10932
                     /gene="uvrA"
                     /locus_tag="PC545_002352"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2457119.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLITTLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFASTNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPKEVSKVDGSYTGQFLKKYFS"
     gene            11191..13008
                     /gene="uvrC"
                     /locus_tag="PC545_002353"
     CDS             11191..13008
                     /gene="uvrC"
                     /locus_tag="PC545_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDF2457120.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSSILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEEN"
     gene            13008..13937
                     /locus_tag="PC545_002354"
     CDS             13008..13937
                     /locus_tag="PC545_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDF2457121.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            14151..15947
                     /locus_tag="PC545_002355"
     CDS             14151..15947
                     /locus_tag="PC545_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HDF2457122.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVLSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVILE
                     RQRAIKELLSKDDFSIHIQTLSNIIGKEQKNNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTATILSFIFCMLGFLPVLPVFLLFIIQLGFSGFGYPRLMQTLAPLFSFSRSIQ
                     VYEKMFEVLEKETFESAYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQILYIIA
                     CGVLMWNYHCAEALERWKGIYGNQIRDWFESIGEFEALISLTVVSHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSKPWVISMVTTHDFELCDLSGSGDVEVVNYHFSEY
                     YVDDKIHFDYKIKDGRCKTTNAKQLMRMAGIL"
     gene            complement(16087..17868)
                     /locus_tag="PC545_002356"
     CDS             complement(16087..17868)
                     /locus_tag="PC545_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HDF2457123.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCVYGECPQECACEEAK"
     gene            complement(17891..19777)
                     /locus_tag="PC545_002357"
     CDS             complement(17891..19777)
                     /locus_tag="PC545_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDF2457124.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            complement(19794..20291)
                     /locus_tag="PC545_002358"
     CDS             complement(19794..20291)
                     /locus_tag="PC545_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HDF2457125.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            complement(20609..22090)
                     /gene="cls"
                     /locus_tag="PC545_002359"
     CDS             complement(20609..22090)
                     /gene="cls"
                     /locus_tag="PC545_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2457126.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKVGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
BASE COUNT         8524 a         2965 c         3697 g         6960 t
ORIGIN      
        1 ggtaaattta taaaaattaa aaaattttta ttttttatgt ttaaaccttg taaatttggg
       61 acataatata attgtaaaac ttaatatagc aaaaataaat tactcaatct cccccactta
      121 aagacctcta tcccccatag aggtcttttt tactttcaaa tcaaattata taaaaaataa
      181 atagttttat atgactcttc ataaattaaa attacattgt gatagcaaac aatgaatatg
      241 aaaaatatag aaaattatag ctaacataag ctgtatatca tcttaacctc agtatattta
      301 acaaaaaatg tttataattt aacctttatt ttaataagtt taccatatat tcactttgtc
      361 ttaatatatc ctttacaaat taagagtatt attaatctat aagtaaatcg tagaattaaa
      421 ttaaatattt tgggaggata aaaatggcag atataagact agaaaatgta acaaaatctt
      481 ataataaaaa aaatacagtt atagaaaatt taaatcttac tatagaagat ggttctttta
      541 cagtattggt aggcccaagt ggatgtggta aatctactac gttaagaatg attgcaggtc
      601 ttgaagatat aacttcaggt aggttgaaga ttgaagaaaa tgatgttaca aaaacggatg
      661 ctagtaaaag ggatatagct atggtatttc aaaattatgc attatatcca catatgactg
      721 ttagagaaaa catagaattt ggtcttaaaa acaaaaaagt agaaaaagaa aaaagagaaa
      781 aacttataac agaagttatt gatgtagtag gattgagaga atatttaaat gtgaaaccag
      841 gcaacttatc tggaggtcaa agacaaagag tggcacttgc tagagctatg gttaaaaatc
      901 caaaggtatt tttaatggat gaaccattgt caaatttaga tgctaaatta agaaatcaaa
      961 tgagaacaga attaatacaa ttacatcaaa aactaaagtc tacatttgtt tatgtaactc
     1021 atgaccaggt tgaggcaatg tcaatggcag ataagatagt tataatgaac aaaggaaaga
     1081 taatgcaaat aggttctcca aagaagatat atcaaaatcc taaaaattta tttgtagctc
     1141 aatttatagg gagtccttgt atgaacatac tagatataaa aaatgatatt aaatttggat
     1201 tcagaccaga aaaagcaaca ctagagagta taaacataga tggaaaaaat gatgatgtat
     1261 ttagtctatt tggaaaagtt gtaactaaag aagtgttggg aaatgaaact atatattgtt
     1321 tatctgttct taatagagag ataagagtca aaacagaaaa tgatatattt gatattggaa
     1381 gaactttaag aataagtgta ttagaaaaag atttatatta ttttgataaa aatggagaaa
     1441 gaatagaaga tgtaaaagta tgtaaaagta tgtattctaa aattaaagga gatatttatg
     1501 aaaaagcaat ttaataaagc atatttatat ataacacctg ctattatagg aactttattg
     1561 tttatagtct atccaataat taagacgata tctctaagct tcaaatctgg tagtcttctt
     1621 agttcaacct ttgaaaatgt aggcttaaac aattatacag aacttttaaa gaatccagag
     1681 tttatacaaa ctataacaaa cactgcaatt tatacttttt ttatggtgac tatatctata
     1741 tcactagcca tagtacttgc tgtttggcta aataaaaatt ctataataca caatttaacg
     1801 caaagtataa tctttacacc acatgtaatt tcactagtat ctgtagctgt attgtggctt
     1861 tggattatgg agccacaata tggcttttta aactggatac tatcgacatt acatttacca
     1921 acatcaaagt ggctttcaag tgaaagttca gctttaatgt cactaattct aataggaata
     1981 tggaagactc ttggatataa tatcttaata gtgttatcag gtcttaaatc cataccaaac
     2041 tatatatatg aagcatctaa attagataat gcaaacaaaa taacgaattt ctttaaagtg
     2101 acattgccac tattatcacc aacaatattt tttcttatga taacaacaac aactgcatct
     2161 tttcaagtat ttgatgagat aagtataatg acacaaggtg ggccagtggg ttcaactaac
     2221 atgttatctt attatatata tgagtctgga tttatatact atgacatagg gcaagcgagt
     2281 gcagcgagta tgtttttgtt agctatagtt atgattgcta catatgctaa ctttagactc
     2341 ttatcaaaga aagttcatta tcaataaaga aaaagtagat attataaggt ttatccaaaa
     2401 ggaggagaca atggatatta tacaacctat caaaaaagac aaagttgata taaaaaaaat
     2461 agcatcaaaa tcaatagaaa tattaattct attactttta tctgcaatat ttatattccc
     2521 ttttatatgg atgctatcta catcctttaa aaatccacaa gaaatgatgc aattaccgcc
     2581 cactattata ccaaatgata tagtgttaga aaattatagt acagcatggc attcaggtcc
     2641 attttttaga tatttgctaa acagtgtatt tgtaacagga agtgttttaa ttgtacaatt
     2701 tttagttatg gttccagcag catatgcatt ttctaagatt aaatttaaag gtgaaaaaat
     2761 gctatttggg cttttgcttg taggacttat gattccacca caagtaactt ttctaccagt
     2821 ctatatgatg atgagtaagt ttggatttat aaatacttat gttccactta taattccatt
     2881 tatgacatct tcatttggga tatttctgct tagacagaat tttatgcaga tttcagatga
     2941 aattattgaa gcagctaaac tagataatgc ttctgactta aaaataatgt ttagaataat
     3001 ggttcctatg gcaaaaccag cagtaataac atttatatta tttaatttta tataccattg
     3061 gaataattat ttctggccct tagtaatgac aaatacagat gcaataagaa ctttaccaat
     3121 aggagttgcg ctccttaaat cttcagaagg aattactgca tggaatgtaa taatggcagg
     3181 aaatataatt ctaattttac caataattct tgtttatata tttgcgaata aaaaagtaaa
     3241 agaagctttt atgtattcag gaataaaata aaaaattaaa gactaaattt ggaggatgaa
     3301 aatgaagctt aaaagaaaat tagcaacagg attagcaata acattattat taggttcttt
     3361 agttgggtgt tcaaattcta atgatgcaaa tgcatctaaa ggagaaaaga ctgatagcaa
     3421 tattacgaag atagatttct gggctccttt aggtggaact aatggagaaa ctgctcaaaa
     3481 aatggttgac caatttaata gtgaacataa ggatgtacaa gtaaatatgt taaaacagaa
     3541 agattactat gaaaatgcaa caaaactaca ggcagcctta acatctaaag accaaccaga
     3601 tgtaacatta ttagaaataa ctcaaacagg tacattcgca tcagcaggtg ctttagtaga
     3661 tatgagtaaa tattttgata aaacatatca agaaagattc ttttctggtc ttttaacaaa
     3721 ttcatattat gaggataagt ttgttggtat gccatttaat agaagtacac caatattata
     3781 cataaataaa gatatggcaa ctaaagcagg tcttgaccct tcaggtccaa aatcatggga
     3841 agagcttaag gattatgcta gtaaaatgac aaataaatca gaagatactt atggttttga
     3901 aacaccaata gatatttggt tctatgaagc tatggtaatg caaagtggag gagaaataac
     3961 taatggtaaa aaagtagcat ttaataatga ggcaggacaa gcaccagtta agttctggca
     4021 agatatgatg aaagctggaa taatgaaaat gcctcctgga gaagattata atgcctggga
     4081 cgtagcaaaa caagattttg taaatggtaa agtaggtatg atatttacat caacagcaga
     4141 tttagcagga cttatgcaac aaacagaagg taagtttgaa atatcaacag cattcttacc
     4201 aaaaaatcaa aaatacgcaa ctcctacagg tggagctaat ctagttatgc ttgaaggtgg
     4261 aacagataaa gaaaaagaag caagtgcaga atttatagaa tggatgacac aaacagataa
     4321 aatcgttcag tttagttcat caacaggata cttaccaaca actgaagatg caacaaactc
     4381 agaaaagtta caaacattat ataaagaaaa acctcaatat aaggttgcaa ctgaccaatt
     4441 acaatatgct gttgcacttc caatgttaaa tggatacaag gaagcaactg ataagctaat
     4501 ggatgagatt aaaaaggggc ttactgattt aaatgcaaat ccaaaagatg tagtaagcaa
     4561 aggaacttct gcaatgcaag cagtaataga taaaacaaat aaataattat atagacttgt
     4621 aagttgatta aaagtattca ataatatata aatgaagagg taatgtaaat gacaaaggtt
     4681 atagtattat cagtagattc tttatttgaa aaagacttgg aatttgtaaa aaatctacca
     4741 aactttaaat ctatactaga aaactgctcc atagtaaaaa atataaattg tgtttatccc
     4801 actctgacat atccgtgtca tactagtatg gttacaggag tatatcctaa aaaacatggt
     4861 atatatcata atgaaaaatt tgacccaaat aaagaaaata aagactggta ctggtactca
     4921 aaggacataa aagcaaaaac tattatagat gtagcaaaag aaaataatct aactacctct
     4981 agtgtattat ggcctgtaat gggagcaaat ccaaatatag attataatat tgcagaaata
     5041 tgggctccta gtagagaaga tgacccaaga gaaacttttg aacgaagttc cagtaaggtt
     5101 ataatggacg gtatttacaa tagacattgt cattatatcg attggaaatt agaacccaac
     5161 atggatttat ttggagtaaa ctgttcagta gatataataa aggaatataa accagattta
     5221 atgctaatac atttagctac attggaccat acacgacaca attatggctt atttaatgac
     5281 gaagtagata aagcattaaa aatgaatgat aaatggttag gagatattat tcaggctaca
     5341 aaggatgcag gtacatacaa agatacaaat tttataatac ttggggacca tggtcatctt
     5401 agagttgata aagttataaa tccaaatgtc ttgcttaaat caaatggatt tataaaagtt
     5461 gaaaatggag aagttaagga ttttgatgcc tatgtaaatt cttctggaat atcagctcaa
     5521 ataatagtaa ataacttaga taggttaaca gaattaagag aactgcttga agaatttaaa
     5581 gaagaattat tcatagagaa tatttttacc aaagaggaag catctaacct tggtcttgaa
     5641 ggtgattttg aaatggtaat tgaaggtctt gaaggaattt catttggaaa tgatttcgaa
     5701 ggaagtatta taaaagattc agatataaaa gattacaagt ttgctgttgc gacacatggt
     5761 catttaccta ctaaaggtaa tagacctcca tttatagctt ttggaccaaa tattaaaggt
     5821 ggtgtggtta tagaagaagg agacctgaga gcacatgcag caactatatt aaaaatgttt
     5881 aatttaaaat tagatggagt tgaaaaagaa gcatttgatt ttattatgta agttgaataa
     5941 agtatttatt ttccatgaat tatagacttc tattgcaaag atagaagtct ttttttatgt
     6001 tttagattta agtaaaattt aaatgggtaa aatatttaaa ataacttaaa caattttata
     6061 aaggtatgat ataataatac atgtcaggaa ctaatgttcg aaaaaaggag tgatactatg
     6121 gattttaaaa taaaatcaga cttcaaacct acaggtgacc aaccagaagc tattaaatca
     6181 atagtggatt ctataaatag aaatgagaaa ttttcgacat tattaggtgt aacgggttca
     6241 ggaaaaacat tcacaatggc aaatatcatt caacaagtta agaaacctac cctaataatg
     6301 gctcataata aaactctagc agcacaactt tacagtgagt ttaaagaatt tttcccagac
     6361 aatgcagttg aatactttgt aagttactat gattattatc aaccagaagc atatgtagct
     6421 cacagtgaca cttatataga aaaagatgca agcataaatg atgaaataga taaacttcga
     6481 cattctgcta cagcatctat tttagagaga agagatacta taataatatc atctgtatct
     6541 tgtatatatg gacttggtga cccaaaagac tataaagaac ttatgttatc tataagacca
     6601 ggtatgcaaa gagacagaga tgatgtaata aaaaggttaa tagagataca atacgaaaga
     6661 aatgacataa actttacacg aggtaccttt agagttagag gagatatact agaaatattc
     6721 ccagctagta atgatgaaaa agcaataaga atagaatttt ttggtgatga ggtagataga
     6781 ataacagaaa ttgattatgt aacagggaag attgttggta ctagaaatca tgttgttata
     6841 ttcccagcat ctcactatgt aacaacgcca gaaagaattg aaaaggcaat tgtagaaata
     6901 gaaaacgagc ttcaagaaca aattaaattt ttcaaggaaa atgataggct attagaagca
     6961 caaagaattg aacaaagaac taagtatgat atagaaatgt taaaggaaat aggattttgt
     7021 caggggatag agaactattc tagacatatt acaggaaggt cagaaggaga aagaccatat
     7081 actttaatgg atttcttccc agatgattat ttgataattg tagatgaggc acatgtcact
     7141 ataccacaag taagaggtat gtatgcagga gacaggtcta gaaaaacatc acttatagag
     7201 aatggtttta gattaccttc tgcacttgac aatagaccat taaattttca agaatttgaa
     7261 ggaaatataa atcaaatgtt gtttgtaagt gctacaccag ggccttatga aattcaacac
     7321 tcagagacaa tagcagaaca gataataaga ccaactggcc ttttagaccc aattgtagaa
     7381 gtaagaccta taaataatca aatagatgat ttggtaggag aaataactaa aactatagaa
     7441 aaaaatgaaa gagtgcttat aaccacttta actaaaaaaa tgagtgagga cttgactaac
     7501 tatcttaaag aaatagggat aaaagttaaa tatttacact cagatatagt tacattagaa
     7561 agaacagaaa taataagaga tttaagatta gggaagtttg atgtccttgt aggaataaac
     7621 ttacttagag aaggattgga tataccagaa gtttcactta tagcaatttt agatgcagac
     7681 aaagaaggtt tcttacgttc cgaaacagct ttaatacaga ctattggacg tgcagctaga
     7741 aatgaaaatg gaagagtaat tatgtatgca gacaggataa ctgactcaat gcaaaatgct
     7801 attgatgaaa caaagagaag aagagatata caaaatttat ataatgaaga gcacaatatc
     7861 attcctaaaa ctatacagaa aaatataaga gatagtatag aagctactaa ggttgcagag
     7921 gaagaagttg tatatggaat atctgataca gatgataaag acgaaataag agctaatata
     7981 gataaattaa aatcagagat gatggaagct gctcaaaact tacaatttga aagagcagca
     8041 gaacttagag ataaagtgaa acaattagaa gaaaaattgg aaaaatagaa aggtaaaaac
     8101 aacattatgg aagataaaat tataataaga ggagcaaaag aacataattt aaaaaatata
     8161 gatttagaat taccaagaga taaatttatt gtttttacag gtttgtcagg gtctgggaag
     8221 tcttctttgg catttgatac aatatatgca gaaggacaga gaagatatgt agaaagtcta
     8281 tctgcttatg caagacaatt tttaggtcag atggaaaagc caaatgtaga atatatagag
     8341 ggtttatcac cagctatctc aattgaccag aagactacct caaaaaaccc acgttctacg
     8401 gttggaacag taacagaaat atatgattat ttgagactat tatttgcaag agtaggagat
     8461 gtgtattgcc ctacatgtgg tgaaaaaata agtcagatga ctattcaaga aatagttgat
     8521 aaaatgctag attttccaga tagaacgaaa ttacaaattc tttcaccaat tgttagagga
     8581 caaaaaggta cacataaaaa agtctttgat aatatcaaaa aagaaggatt tgtaagagtt
     8641 agagttaatg gtgaaaacta tgaagttagt gatgatataa tcttaaataa aaatcaaaaa
     8701 cataatatag aagttgtagt ggacagaata gtaattaaag atggaataga atcaagacta
     8761 gcagattcaa ttgaaactgc agttaagcta tctgatggtc ttgtaatagc ccaaataata
     8821 gatggagaag aaattctttt ttcaactaaa tttgcatgcc cagaacatgg aattggcata
     8881 gaagaattat ctccaagaat gttttcattt aatgctccct ttggtgcttg tgatgtttgt
     8941 aatggtcttg gggaaagtaa agaagttgac ccagaattag tagtaccaaa caaagattta
     9001 tcaataaaac aaggtgcagt ggcagcatgg ggttcaacag gtgttaatga tgacacttat
     9061 tacagtaaaa tgataaaaag tttagcagag cattttaatg tatctttgac aacaccgttt
     9121 aaagatttac cagatgattt tgtggaggaa ttactttatg gtaaagataa tataattgta
     9181 gagtttactt atgagtcaaa atttggagga actagaaatt ataaatcata ctttgaaggt
     9241 gtaatagtaa acttagagag aagatataga gaaactaatt cagattacat gagagataag
     9301 attgaagaat acatggcaga gagaccatgt ccaaagtgta aagggatgag acttaaaaag
     9361 gaagttttat ctgttttagt tgatggaaaa aatataatgg aagttacaaa cttatctgta
     9421 aatgaattga ttgactttat ggaaaatata aatcttacag aaaaacagag atttatagct
     9481 catgagatta taaaagagat aaaaggaaga gctatgttct taagagatgt aggtcttgat
     9541 tatttaaatt tatcaagaaa agctggaact ttatcaggag gagaagcgca acgtataaga
     9601 ttagctactc aaataggttc tgcattggtt ggagttttat atgtacttga tgagccaagt
     9661 atagggcttc accaaagaga taatgatagg cttataacta ctcttagaca tctaacagat
     9721 ttaggaaata ccttaatcgt tgtagagcat gatgaagaca caatgagaga agctgattat
     9781 gttatagaca ttggtccagg tgcaggtata catggtggtg aaatagttgc acagggtact
     9841 ttagatgaaa taattgaaaa tgagaactca ataacgggtc aatatttaag tggtaaaaaa
     9901 caaatattgc ttcctgagac tactagagaa ggaaatggaa gttttataga aatagttaaa
     9961 gcatcagaaa ataatctgaa aaatattgat gtaaagttcc cattaggtaa gtttacatgc
    10021 ataacaggtg tttcaggttc tggaaaaagt acattaataa acgatatact ttacaaaggt
    10081 gttgcaagta aagtgaatag acttaagcaa agagctggta agcataagga gataaaagga
    10141 attgaaagta tagataaagt tataaatata gaccaaagtc ctattggaag aacacctcgt
    10201 tcaaatcctg ctacttatac aggtgtattt gattttataa gagatatttt tgcatctact
    10261 aatgaagcta aagcaagagg ttataaaaaa ggtagattta gttttaatgt aaaaggtggt
    10321 agatgtgaag cttgtaaggg tgatggaata ataaaaatag aaatgcattt cttaccagat
    10381 gtatatgttc cttgtgaaat ttgtaaaggt gaaagataca atagagaaac attgcaagta
    10441 aaatacaaag ataaaactat atctgatata ttagatatga atgtggaaga agctgttgag
    10501 tttttcgaaa atataccaaa tataaaaaga aaattagaaa ctttaatgga tgtaggactt
    10561 tcatatatta aattaggaca gccttcaact caattatcag gaggagaagc acaacgtata
    10621 aaattagctg ctgaacttag taaaagacca acaggaaaga ctttgtatat attagatgaa
    10681 cctactactg gacttcatat ggcagatgta gacaagctaa tccaagtatt acaaagacta
    10741 gcagatacag gaaatacaat tatagtcata gaacataatt tagatgttat aaagacttgt
    10801 gattatgtaa tagacttagg accagaaggc ggagataagg gtggaactat agttgcaact
    10861 ggaacaccaa aagaagtatc taaagttgat ggttcttata caggtcaatt cttaaagaaa
    10921 tatttttctt aagttatata aattgtgaaa aattttagag cagatatacg ataaactgtt
    10981 ttgttataag taaattagtc aaatataagt tgtaaatagt aatacaaagt aaagtccata
    11041 ttactcttca tggttaaaat tgttttcaaa tcaattatag aagggaatat gggctttaac
    11101 ttgtttaaag cactggaaat tgtataactt tttaaaattc atattttagg gtataatttt
    11161 agtataaaat ttaatgtttt ggagggcgag atgtttgata tacaagagca tttaaaaaaa
    11221 ttaccttctg agcccggagt atatttaatg aaagacaaat acgaccatat aatatatgtg
    11281 ggaaaagcca tatctctaaa aaatagggta aggcagtatt ttcaatcttc aaaaaaccat
    11341 acatctaaag taaaatctat ggttaagaat atatataagt ttgagtatat tataacggat
    11401 tcagagttag aggcattaat acttgaatgt aatttaataa aaagatacag accaaagtat
    11461 aatgtagtgc ttagagatga taaaacttat ccatatataa aagtaactac gaatgaagat
    11521 tatccaagaa tcttaaaagt tagaagagtt cttaaagata aggcaaagta ttttgggccc
    11581 tataccaata taactgctgt gaatgatacc ttggagctta taagtagtac gtatccaata
    11641 agaagctgta aaatagatat agataaagca ataaagaata agacaagacc ttgtttaaat
    11701 ctacacataa ataaatgttt aggaccttgt acaggaaatg tttctaaaga agagtatgga
    11761 aaaatgatag aagagattat aatgtgttta tctggtaaag aagaaaaatt gatggaactt
    11821 ttaaaggaaa aaatgaatga gagttctatg aactttagat ttgaagaggc tgctgtatat
    11881 agggataaga taaagagcct tgaagagatg atacagaaac aaaaaataga tgctactgta
    11941 agtgacttaa accaagatgt ggttgcaatg gcaagagctc ataatgaagc ttgtgtccag
    12001 gtatttttta taagaaatgg taagattgta ggtagggaac attttatatt agaaggggta
    12061 atggatagtc caagagcttc tatattaagt tcatttgtaa aacaatttta taatgaacaa
    12121 gagtatatac ctaaagaatt aattatagaa gatgagatag aagatagttc tatattagaa
    12181 gaatggctgt catctaaaaa ggggcaaaaa gtaacaataa gagttcctca aaagggagag
    12241 aagaaaagct tagttgaaat ggttaggaaa aatgctgtag aatatcttga aaaattttct
    12301 gatatgaata aaagaaaata tgaaaaaagt attggagcat tggaagaatt aaagcaaata
    12361 ttaaatctag aaaagttgcc aataagaata gaggcttatg atatatcaaa tatacaaggt
    12421 gtagattcga ttggttctat ggtagtgtat acgaatgcta aaaaagataa gaaggaatat
    12481 aggagatata aaataaagac agttattgga cctaatgatt atgattctat ggctgaaatt
    12541 gtagatagaa gattaaaaca tggaaattta cctgacttga tattgttgga tggtggaaaa
    12601 ggacaagtta gtgcagtaaa aaaagtacta gaactcaatg atgtagatat acctctttgg
    12661 ggaatgtaca aagatgataa gcatagaact aaaggattga tatgcaaaga aaaagaaata
    12721 gaactagata aaacaactaa tttatataga tttattgcta gtatacaaga agaagttcat
    12781 aattatgcaa taacatatca tagaagtcta agaaataaag ctttgacaaa atctatctta
    12841 gatgatatac aaggaatagg tgaaaaaaga aagaaatcat tattaaatca ctttaaagat
    12901 gtagatgcta taaaaaaagc tactatggaa gaacttttag aagtagatgg aatgaataaa
    12961 agcattgcag ataatgttta taattttttt agaaaggaag aaaattaatg gaaactagca
    13021 ataaagtatc aataagagag ataataaaag agttgaagtt aaaagtagta tacatgcctg
    13081 atgatgtaga ttattatgtc tactctcaag atataaatag accaggatta cagtttgcag
    13141 gatattttga ttattttgca tatgaaagaa tacaaatagt tggaaagaca gaatataatt
    13201 tttttggatt aatggatagt catattcggg aagaagtact agatagattt ttctcatatg
    13261 aaataccagc gttaataatt tctagagact tggaaatcaa gccagatgta attgaaaagg
    13321 ctaaaaaata taaaagaata cttttgagca caaatagaaa taccactaga cttataaata
    13381 gactgtcaaa ctatttggat aataagttag caccacacac aacaattcac ggggtgctag
    13441 tagatattta tggtataggt gtactgataa aaggtgaaag tagtatagga aagtcggaaa
    13501 cagcattaga attgattcaa aggggtaata gattagttgc agatgatgct gtagaaataa
    13561 gaaaattaga tgaaagtatg cttgtgggtc agtcacctga actaataaga cattttcttg
    13621 agataagagg aataggaata atagatgtta gaagtttata tggagtaggg gcaatcaaaa
    13681 attctaaaat gatagattta gtagttcatt tagaagcttg ggatgaaact aagtattatg
    13741 atagactggg actggataaa gaatatgaag aaatacttgg tgttaatgta gagaaattag
    13801 ttgtacctgt taaacctgga agaaatactg ctatgatatt agaagtagca gcaatgaatt
    13861 ttagacaaag aggaatggga tacgatgcag ctcaagaatt tactaaaaaa ttatcaaaat
    13921 taatcgatac taagtagtac tgaataatag tgaactacac atcatttaat taaatattga
    13981 ttaggattat gtcctacacc ttataaatta aaatcaagtt gtatatatat aatgtttatt
    14041 aataagatat attatataag gataatttaa agtacagtgt tagattataa taatactaga
    14101 ttaattttat atttgagttt cattatataa caagaaagtt ggaatttttt atgggaatag
    14161 aatattttag taaactttta gaaagtagta ctgaaaaaga attgctctta agtaaaaagt
    14221 caaataacat aagtacagca agattaatat cttttttgat agtgatagca gggtttgcta
    14281 ttggatttta caataaaaat atagtaggta tttttgttgg agttttatca ataataatat
    14341 ttatagcact cttagttatt catagtaaag taaaagaaga agaaacatat tttaaaagta
    14401 agagcgaagt attaaataag tatattaaga gatttggaga tggttggaag gaatttaaaa
    14461 tagatggtaa agaatactta aaagatgaaa attcgcaagc aaaagattta gacttatttg
    14521 gaagagcatc attgtatcaa tatatctgtg tagctaatac atcttatggg aaaaattttt
    14581 tagccaaata tttgtggaat gaaaatccag atgagaatgt aatcttagaa agacagagag
    14641 ctataaaaga acttttatca aaagatgatt tttctattca tattcaaaca ctcagtaata
    14701 taattggtaa agagcaaaaa aacaattcag atagtagtat agaatcgttt atagaatatg
    14761 gagaaaataa gaatgtgtat ataccaaaat ggatgcatat ttttacttgg ggtttaccaa
    14821 ctgcaacaat cttatctttt atattttgta tgttaggatt tttacctgtt ttacctgttt
    14881 ttttactttt tataatccag ttagggttta gtggttttgg atatccaaga cttatgcaaa
    14941 ctcttgctcc attattttca tttagtagaa gtatacaagt atatgaaaaa atgtttgagg
    15001 ttttagaaaa agagacgttt gagagtgcat atttgaaaga attacaagat aaactttcta
    15061 agggaagtgg agtttcaaga ggtgttaaac aattaaattc aataggaaat gctgtaaacc
    15121 taaggtataa tcagatacta tatataattg cctgtggtgt tttgatgtgg aactatcatt
    15181 gtgcagaagc tttggagcga tggaaaggta tatatggaaa tcaaattaga gattggtttg
    15241 aatctattgg agaatttgaa gctctaataa gtttaacagt tgtttctcat gtaaaggaaa
    15301 atacatgttt tccagtaata aaatatgaag atactccaag attgaaagtt gaagaggtat
    15361 accacccatt aatagcagaa aaaagtgtca tagcaaattc gatacaatta aattctcaga
    15421 cttgtattat cacaggttca aatatgtctg gaaagacaac atttttaaga agtatcggtg
    15481 taaacttagt gctggcatat gcaggtgctc cagtatgcgc taagagtttt gatgcgacct
    15541 gtatggctat atttacttct atgagaatac aagatgatgt aagtcaagga atatcaactt
    15601 tctatgctga gattcttaga ataaaatcaa tgattcaata tagtttaaaa gaactgccaa
    15661 tgttggttct agtagatgaa atttttaagg gaactaactc tgctgataga attattggag
    15721 caagtgaggc agtaaagaaa ttatcaaaac cctgggttat aagtatggtg acaacacatg
    15781 actttgagct atgtgacctt tcaggtagtg gagatgttga agttgtaaac tatcatttct
    15841 cagagtacta tgtagacgat aaaattcatt ttgactataa gataaaagat ggaagatgta
    15901 aaactacaaa tgcaaaacaa cttatgagaa tggcaggtat tctttaatta atagataggt
    15961 taattaaaga ataagtctat ttagaaatat aatttaattt gtgaaattaa taaggattgt
    16021 cttaaattac gtaaaagtat tttaagacaa tcctttttta agaataaatt ttatattttg
    16081 ttatgactac ttagcttctt cacaagcaca ttcttgtgga cactctccat agacacaact
    16141 cttatcaaag taatgagtat gaaggtattt atgggctaca tcactatttg gttctccaaa
    16201 gaagtcttta taaatagctt gtaaatcagg attctcatga gatttacgaa tagccttatt
    16261 tctatctatt tcatataacg catttgcacg tctatcaact atttcagtat taccatggtg
    16321 ataaggttga ccagcacctc caacacatcc accaggacat gccataactt ctatgatatg
    16381 gtatttacat tctccacttc tgaccttatc cataatcttt ctagcgtttt taagtgtatt
    16441 tacaatacac acattaactg tagttccatc aacatctata cttgcttccc taattccttt
    16501 aagacctcta acattagtga agtttacatt atctaaagtc ttattagtta tcttctcata
    16561 tgaagtacgt agggcagctt ctaaaactcc accacttgct ccaaatatag atgcagcacc
    16621 tgtagatttt cctagaggat tatcaaaatc ttggtcttga agattaggta aatcgattgc
    16681 agcttctttt atcatcttag ctaactctct agtagttata gaaaggtcaa catctagtat
    16741 tccagattca cttaattctt ctcttgaagc ttcatatttt ttagctacac aaggcattac
    16801 agatacaaca taaagttctt caggattgat tcctaatatt ttaggtgcat agtagttttt
    16861 agctatagaa ccaaacatac cttgaggaga cttacagcta gatgctaagt ttaacttgtc
    16921 tggataattg tgctctaaga agtttaccca tgcaggacaa caacttgtta atataggaag
    16981 attttcacct ttttggattc tttctataaa ttcagtagct tcttccatta tagtaaaatc
    17041 tgcaccaaag tttgtatcaa atacatgttc aaatccaagt gcttttaaag cagctaccat
    17101 tttccctgta gagattgacc ctggttctaa tccaaactct tcaccaatag caactcttac
    17161 agcaggagca acttgcacta ctatagtctt ttctttttta tttaaaacat cccaaagttt
    17221 aggtacatta tctacttctg ttaatgctcc agttggacag acagatatac attgaccaca
    17281 gaatgtacac tctgtttcaa ccatatcaga gttaaagaat gtactaacta aagtattaaa
    17341 tccacgatta actccagata atgctcctac tgtttgtatg tcattacata cagtctcaca
    17401 tcttctacat aatatacact tagaatggtc tttaactaaa gatttagtag aagtatcttt
    17461 acctgcaaaa gatttagaac cttgatatct tatttttctt actcctaaat cagcagctat
    17521 agtctgtaat tcacaatctc catttttttc acagacaaaa caatcttgtg gatggtctga
    17581 taacaataat tcaacaatag ttctacgagc atttaatgct ttagcactat tagtctgaac
    17641 tttcatacct tcctttataa ctgttccaca agctggaact aatcctctct ctgtctcaac
    17701 catacaaaca cgacaagaag cacaagtgtc aagcttatct atttcattca tatgtaaatg
    17761 acaaaggttg tgtattttta tatttattaa tttcgcagca tctaagatag aagttccact
    17821 aggtgctgaa acgtgtttgc cattaatagt taaattaact aaactcattg ttattcctcc
    17881 tccaaataag ttatttcttg attatagcgt taaatgtaca attatctata caagcaccac
    17941 attttagaca cttagaagta tctattgtat gttgttcctt aacagaacct gttatacaac
    18001 cagcaggaca cactctagaa cattttgtac aacccttaca gtcatctgtt ataaagtaag
    18061 aaagtaagtt ctgacattta ccagcaggac atttcttatc tacaacatga gctatatact
    18121 catctttgaa atattgaaga gtacttaaaa cagggtttgg agcagcttta ccaagaccac
    18181 ataatgaagc agtttgtata gtttcagcaa ggttttctag gtcatctaaa tcttgtagag
    18241 taccattacc ttctgtaatt ttagttaata tttctaataa tcttgtagta cctatacgac
    18301 aaggtgtaca tttaccacaa gattcttcaa cagtaaactc aaggaagaac ttggctatat
    18361 ctaccataca gtcagattca tcaagaacaa gcataccacc agagcccatc attgaaccga
    18421 tagaacttaa tgaaccaaag tctataggag tgtctaaatg ttcagttggt atacatccgc
    18481 cagatggtcc acctgtttga acagccttaa atttcttacc ttcagggatt cccccaccaa
    18541 tttcaaatac aatatctctt aaagtagtac ccattggaac ttcaacaagt cctacatttt
    18601 ctacttttcc accaagagca aagactttag taccactaga atcttcagta cctaagttgg
    18661 caaaccaatc tccaccctta agtataattg caggtatgtt agcaaaagtc tcaacgttat
    18721 ttaagcaagt aggagatttc catagaccac tcttagaaga actataagtt ttcattctag
    18781 gttcaccacg tctaccttca attgaatgca ttagcgctgt accttcacca caaacgaaag
    18841 cacctgcacc atattttaat tctaatttaa agttaaatcc agtgcctaag atattttcgc
    18901 caagtaaacc gtatttttca gcttgagcta tggctacttt taatctttct atagattttg
    18961 gatattctgc tcttatgtat atgtatcctg tatcagaacc tatagcgtat ccacatattg
    19021 ccatagcttc tagtacacta tgagggtcac cttcaagtac agatctatcc ataaatgcac
    19081 ctgggtcacc ttcatcagca ttacatacaa caaacttttt atctgttgga ccagctggat
    19141 tttttgaagc agcttcccat ttagagccag tagggaaacc tgcaccacct ctaccacgaa
    19201 ggccagaagt ttttacctct gcaataactt cttgaggagt aagagaagtt aaacatttgc
    19261 ctagagctaa ataaccatca ttggcaatat attcttctat actatctggg tctataagac
    19321 cacaattttt taaagctata cgtaactgtt ttttgtagaa agacatttct tcttgttctt
    19381 caactttttt acttaaagat tcttcctcat ataataattc ctcgacaact gtatttctaa
    19441 ttaagtggct ttgaactatc ttttcagcat cagatggctc aacttttacg tagaaaacgt
    19501 tatcaggata aacttttaca ataggacctt gagcacagaa accaaaacaa cctgtcaaac
    19561 gaacactaac tttatcttgg attcctgatt tctttatttc attttctaat tcactaacta
    19621 tttctagact gtttgaagat gtacatccag tatcacaaca aacaagtaat tctcttctta
    19681 atacattttc cttagaataa ttttttctta agctaagatt aggtttcagc tcatcagcta
    19741 aaacttttaa ttcatcaaat gaatttactt tacgcattat ttattcaccc ctcttaacaa
    19801 ttcaattcta attctctata agtatctaat atttcgctta cttggtcagg tttcacttta
    19861 ccataaactt taccatttac agtaacaact ggagctaaac cacatgcccc caaacatctt
    19921 aaaccttcta aagaaaattt aaaatcacta gtagtttctc caacttttat gccaagctgt
    19981 ttttcaaaag cacttaagat tttgtcagca ccttttacaa aacatacagt tcctagacag
    20041 acactgattt tatacttacc tacaggttct gtagtaaaat aagaatagaa tgtaacaacc
    20101 ccatatacct ttgcaggagc tactccaagt tttctagcaa catgaagttg aacttctttt
    20161 ggaagatatc caaatatacc ttgggcttca tgtagaactt gtattaacgc accttccttt
    20221 gttgcaagag agtcaataaa tatatctagt tcgtcaaata attgcttgtt gtgtgaaata
    20281 aaatcgcaca taacaaatcc cccttttctt tttaaagtta ttatttttca taaaataatt
    20341 aaccatgagt tgaccccaac attatgtggg tgaacaaaac caaaacaata aagaaagaat
    20401 aatatacaac aatttcttta tttataaaaa caatgcaaaa aagttaccta ttaaaaataa
    20461 aaactatact agtctattat aatataaaat aattcagaat agttaaaaaa aataaaaaaa
    20521 atgagctaaa ttcaataaaa aaaaaataat acatataaaa aactttaaaa aataaaccaa
    20581 aatgtaaaat ttttataaaa taaaagattt ataaaactgc tgataatagt cttgaaattg
    20641 attccttgat ttttatataa gtagacctag aattatatat atctaatgtt atttccttag
    20701 aatttaaaat atcattttca aatataattc tttgttccaa tgcttttttt gaagagtaca
    20761 taaatgcatt aacttcgaaa ttaagctcaa aacttctgat atccatatta gctgttccta
    20821 tagagcatat agagtcatct ataactattg tcttagcatg taaaaatgca ttttgaccat
    20881 aagtatagat tttagctcca aactttaaaa gttctccagc atatgaatat gatgcccagt
    20941 atacaaaggg atggtcaggt ttagaaggaa tcataactct cacatcaaca ccagataaac
    21001 aagctatttt taaagtatct ataaaagttg agtcaagtat taaataagga ctctgtatgt
    21061 atatatactt tctagctttc tgaatcattt taaggtaacc atatttaatc tcatcaagtt
    21121 ctgatatatc tggaccactt gatactatct gaatcccaat attttcagaa ggatgattag
    21181 aatttgtatc ttcttctata aagtactttt ctaaatctaa gtcttcttta gttgtatatc
    21241 tccaatcaag gatgaatctc atatttaggt caattacgca atcaccagta agcttagtat
    21301 gtgtatctct ccatagacca aattttggat ttctacctaa atattcatca ccaacattat
    21361 atccacctac aaatcctacc ttaccatcaa tgacaacaat ttttcggtga tttctatagt
    21421 ttaagttaaa gtttacaatt ttcaagaatg atgggaagaa gcttccaact ttaacaccac
    21481 tttctttaag tctggaaagt gtactatttt ttagtgttct accaccaact gcatcaaata
    21541 aaagtcttac ttcaacacct tctttagctt tgtctataag aatgtctatt atttcagttc
    21601 cgattttgtc atctttgaaa atgtaaaatt gtatgtttat atatttttta gcttttttaa
    21661 gttcttcaag aagactgtta aaaaattgac tagattcagc ataaatccac acatcgttat
    21721 tattggtata atgagcatta tttgaattag ccaatgcata aatcatatcc ttatgttgat
    21781 gtatatctga atcaatttca gatgtagatt ggagtttaac ctgagtatct agtatattac
    21841 ttttgattat cttatcatcc ttttctttca atctaaacat attgtttttt gaaatatttc
    21901 tgccaaaagc tatataaagt atgaatccaa gagcaggaat caatactaat ataagaagcc
    21961 atgttaaagt tgtatttatg tttctttttt ctctaaagat aaggtttagt ataaccatga
    22021 aattaattat atatattgta gtaactgcta tttcatatat tgacatttcg aaaaatgata
    22081 tatcaaacat aaaattccct ccttcaaata tttattaaaa taagtaatta tatataataa
    22141 cacaaa
//
LOCUS       DAMJVI010000062        21942 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.064, whole genome shotgun sequence.
ACCESSION   DAMJVI010000062 DAMJVI010000000
VERSION     DAMJVI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.064"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            370..681
                     /gene="rpsJ"
                     /locus_tag="PC545_002360"
     CDS             370..681
                     /gene="rpsJ"
                     /locus_tag="PC545_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDF2457127.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            773..1402
                     /gene="rplC"
                     /locus_tag="PC545_002361"
     CDS             773..1402
                     /gene="rplC"
                     /locus_tag="PC545_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDF2457128.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1432..2055
                     /gene="rplD"
                     /locus_tag="PC545_002362"
     CDS             1432..2055
                     /gene="rplD"
                     /locus_tag="PC545_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDF2457129.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNVIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2055..2345
                     /gene="rplW"
                     /locus_tag="PC545_002363"
     CDS             2055..2345
                     /gene="rplW"
                     /locus_tag="PC545_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDF2457130.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTSSFKKAVVKLTADSKEIEFFQGM"
     gene            2375..3205
                     /gene="rplB"
                     /locus_tag="PC545_002364"
     CDS             2375..3205
                     /gene="rplB"
                     /locus_tag="PC545_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDF2457131.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3240..3521
                     /gene="rpsS"
                     /locus_tag="PC545_002365"
     CDS             3240..3521
                     /gene="rpsS"
                     /locus_tag="PC545_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDF2457132.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3553..3888
                     /gene="rplV"
                     /locus_tag="PC545_002366"
     CDS             3553..3888
                     /gene="rplV"
                     /locus_tag="PC545_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDF2457133.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3911..4726
                     /gene="rpsC"
                     /locus_tag="PC545_002367"
     CDS             3911..4726
                     /gene="rpsC"
                     /locus_tag="PC545_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDF2457134.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4762..5193
                     /gene="rplP"
                     /locus_tag="PC545_002368"
     CDS             4762..5193
                     /gene="rplP"
                     /locus_tag="PC545_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDF2457135.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5195..5398
                     /gene="rpmC"
                     /locus_tag="PC545_002369"
     CDS             5195..5398
                     /gene="rpmC"
                     /locus_tag="PC545_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDF2457136.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC545_002370"
     CDS             5422..5676
                     /gene="rpsQ"
                     /locus_tag="PC545_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDF2457137.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5702..6070
                     /gene="rplN"
                     /locus_tag="PC545_002371"
     CDS             5702..6070
                     /gene="rplN"
                     /locus_tag="PC545_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDF2457138.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6094..6399
                     /gene="rplX"
                     /locus_tag="PC545_002372"
     CDS             6094..6399
                     /gene="rplX"
                     /locus_tag="PC545_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDF2457139.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6429..6971
                     /gene="rplE"
                     /locus_tag="PC545_002373"
     CDS             6429..6971
                     /gene="rplE"
                     /locus_tag="PC545_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDF2457140.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEMISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6989..7174
                     /locus_tag="PC545_002374"
     CDS             6989..7174
                     /locus_tag="PC545_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDF2457141.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7207..7605
                     /gene="rpsH"
                     /locus_tag="PC545_002375"
     CDS             7207..7605
                     /gene="rpsH"
                     /locus_tag="PC545_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDF2457142.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7636..8178
                     /gene="rplF"
                     /locus_tag="PC545_002376"
     CDS             7636..8178
                     /gene="rplF"
                     /locus_tag="PC545_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDF2457143.1"
                     /translation="MSRIGVKPIIIPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8214..8582
                     /gene="rplR"
                     /locus_tag="PC545_002377"
     CDS             8214..8582
                     /gene="rplR"
                     /locus_tag="PC545_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDF2457144.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8603..9112
                     /gene="rpsE"
                     /locus_tag="PC545_002378"
     CDS             8603..9112
                     /gene="rpsE"
                     /locus_tag="PC545_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDF2457145.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9127..9312
                     /gene="rpmD"
                     /locus_tag="PC545_002379"
     CDS             9127..9312
                     /gene="rpmD"
                     /locus_tag="PC545_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDF2457146.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9345..9788
                     /gene="rplO"
                     /locus_tag="PC545_002380"
     CDS             9345..9788
                     /gene="rplO"
                     /locus_tag="PC545_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDF2457147.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9832..11100
                     /gene="secY"
                     /locus_tag="PC545_002381"
     CDS             9832..11100
                     /gene="secY"
                     /locus_tag="PC545_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDF2457148.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGEVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11117..11767
                     /locus_tag="PC545_002382"
     CDS             11117..11767
                     /locus_tag="PC545_002382"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDF2457149.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVDYYSKQGIIADIKGDQAIDKVFEDIVAALGSGK"
     gene            11768..12514
                     /gene="map"
                     /locus_tag="PC545_002383"
     CDS             11768..12514
                     /gene="map"
                     /locus_tag="PC545_002383"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDF2457150.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12536..12805
                     /locus_tag="PC545_002384"
     CDS             12536..12805
                     /locus_tag="PC545_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HDF2457151.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12843..13061
                     /gene="infA"
                     /locus_tag="PC545_002385"
     CDS             12843..13061
                     /gene="infA"
                     /locus_tag="PC545_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDF2457152.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC545_002386"
     CDS             13097..13210
                     /gene="rpmJ"
                     /locus_tag="PC545_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDF2457153.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13363..13734
                     /gene="rpsM"
                     /locus_tag="PC545_002387"
     CDS             13363..13734
                     /gene="rpsM"
                     /locus_tag="PC545_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDF2457154.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13770..14168
                     /gene="rpsK"
                     /locus_tag="PC545_002388"
     CDS             13770..14168
                     /gene="rpsK"
                     /locus_tag="PC545_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDF2457155.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14200..14823
                     /gene="rpsD"
                     /locus_tag="PC545_002389"
     CDS             14200..14823
                     /gene="rpsD"
                     /locus_tag="PC545_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDF2457156.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14901..15848
                     /locus_tag="PC545_002390"
     CDS             14901..15848
                     /locus_tag="PC545_002390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDF2457157.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICPPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15869..16210
                     /gene="rplQ"
                     /locus_tag="PC545_002391"
     CDS             15869..16210
                     /gene="rplQ"
                     /locus_tag="PC545_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDF2457158.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16330..17163
                     /locus_tag="PC545_002392"
     CDS             16330..17163
                     /locus_tag="PC545_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2457159.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDDMDTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISSDILTVDEMVMELCQ
                     L"
     gene            17151..18017
                     /locus_tag="PC545_002393"
     CDS             17151..18017
                     /locus_tag="PC545_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HDF2457160.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDVSFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEGFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKDLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFMGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18011..18814
                     /locus_tag="PC545_002394"
     CDS             18011..18814
                     /locus_tag="PC545_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDF2457161.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMVSIFVVNKFWP
                     YIVVLLCLLAMIKLANIPVKYIVKGVKPLKWIILFTFLINIFFLPGDEIWSFGFLAIT
                     KQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18846..19577
                     /gene="truA"
                     /locus_tag="PC545_002395"
     CDS             18846..19577
                     /gene="truA"
                     /locus_tag="PC545_002395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2457162.1"
                     /translation="MRNIKIKIQYNGKNYCGWQKQPDSLGIQGTIERAIYDITKEETS
                     LIGSGRTDSGVHAIGQIANFKINSGISIESIPMALNAKLPKDISVIEACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKSLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDISKKDDLITFEISGNGFLYNMVRIIVGTLVDMGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19697..20128
                     /gene="rplM"
                     /locus_tag="PC545_002396"
     CDS             19697..20128
                     /gene="rplM"
                     /locus_tag="PC545_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDF2457163.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20157..20549
                     /gene="rpsI"
                     /locus_tag="PC545_002397"
     CDS             20157..20549
                     /gene="rpsI"
                     /locus_tag="PC545_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDF2457164.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21008..21760
                     /gene="cwlD"
                     /locus_tag="PC545_002398"
     CDS             21008..21760
                     /gene="cwlD"
                     /locus_tag="PC545_002398"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HDF2457165.1"
                     /translation="MEYYGVFISIHIQGDVVRKYIKHIIFSFAMICLVVVSIFEIKNI
                     SEDVIKYMPVTNKTIILDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGL
                     VILTREDDSSLYKEENNKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYG
                     AQTFYPKDKQDSKALSKCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGF
                     LSNEKECKLLTDETYQEKIAWAIYIGIQKYLS"
BASE COUNT         8811 a         2563 c         4498 g         6070 t
ORIGIN      
        1 ctttactaaa ctaatggatt taatataaaa aaaagagggg gtaactcctc tcttttttgt
       61 tttttgttga caagtagcgc taaagcatat atactaaact agtgccttga gacagattaa
      121 caatcaagtt taaggttatg atgttgtaat agaaaataaa attgaaaaaa acagaaaaaa
      181 aacttgaaaa aatgtaacag atgatataga atataatagt gtgcttgaga gattaagaaa
      241 ataataatta aatcctagtg aaatacgaac tgatatcgat ttaaaagtta caacacaccc
      301 ggagcagtgt atcggtattg gttgtacgca acttgaaaat taaacacgtg ataaaggagg
      361 gaaaacaaaa tggctaagaa tgaaaaaata agaataagat taaaatcata tgatcacaaa
      421 ttattagatt tttcagcagg taaaatagtt gaaactgcta aaaaggctgg atcacaagtt
      481 tcaggacctg tgcctctacc aacagaaaaa caagttgtaa ctatattaag agctgtacat
      541 aagtacaaag actctagaga acaatttgaa ataagaactc ataaaagact aatcgacata
      601 gctaatccaa cacctaagac agttgactca ttaatgagat tagacttacc agcaggtgta
      661 gatatagaaa taaaattata agaaccccta agatattatc ctatagatga ttatcttaga
      721 atgattactc taaaagatgt aatccgctgt aagaattata ggaggtgcta atatgaaagg
      781 aatattaggc aaaaaagttg gtatgactca aatattcact gataaaggtg tagttatacc
      841 tgtaacagct gttgaagcag ggcctatggt cgttactcaa ataaaaacag ttgataaaga
      901 tggatacaat gctatacaaa taggttttga agatgctaaa gaaaaagctt taaacaaacc
      961 taaaaaagga catttagctg ctgctaatgt tttaaagaaa catttaaaag aatttagagt
     1021 agattctgta gaaggataca cagtaggaca agaaataaaa gctgatgtat ttgaagcagg
     1081 tgcaaaaata gatgttactg gaataagtaa aggtaaggga ttccaaggtc caataaaaag
     1141 acatggacaa agtagaggtc ctgaaactca cggttctaga taccacagaa gaccaggttc
     1201 aatgggagct tgttcttacc caggtagagt atttaaaaac aaaaaattag ctggacatat
     1261 gggaagcgta aaggtaacag ttcaaaactt agaggttgtt aaagtagatg ctgacaagaa
     1321 ccttatatta gttaaagggg ctataccagg agctaaaggt tcagtagtaa ctataaaaga
     1381 agctataaag gtttctaaat aatgactaac ctagagaaag gaggaataac aatgccaaaa
     1441 ttaaatgtac taaatgttag tggacaaaac gttggagaaa tagaattatc agattctata
     1501 tttggcgtag aagttaatgg acatgtttta tacgaagttg taaaaaatca attagcaaat
     1561 aagagacaag gaactcaatc tgctaaaact agagcagaag ttagaggtgg cggaagaaaa
     1621 ccttggaaac aaaaaggaac aggtagagca agacaaggtt ctacaagatc tgtacaatgg
     1681 gtaggtggag gagttgcttt tgcacctaaa ccaagaagct acaaatatac attacctaag
     1741 aaggtaagaa gattagctat gaagagtgct ttatcttcaa aagttcaaaa tagtgaagtt
     1801 atagtattag atgcattaaa catggatgct cctaagacta aagaatttgc tcaaatatta
     1861 aacaatataa atgctgctaa aaaagcttta gtagttatag ctgacaaaaa cgataacgta
     1921 ataaaatcag ctagaaatat agaaggtgtt caaactgcat tagttaatac tatgaatgta
     1981 tatgatatat taaaatacga ttcatttata ataactacag atgctgttaa aaaagtggag
     2041 gaggtgtacg cataatgact aatccacatg atgtaataat aagaccagtt gtaactgagc
     2101 acagtatggc tgaaatgggt gaaaaaaaat acacttttgt tgtggctaaa gatgcgaaca
     2161 aaactgaaat aaaaaaagca gtagaaaaag tattcggagt tagtgttgat aaagtaaata
     2221 ctttaaacta cgatggaaaa gttaaaagaa tgggtagaac tcaaggaaga acatcaagct
     2281 ttaagaaagc agtagttaaa ttaactgctg atagtaaaga aatagaattc ttccaaggaa
     2341 tgtagtatta taacaaacga aggagggaaa acagatggct ataaaaaagt ttagaccaac
     2401 ttctcctgcc ttaagacaaa tgacagtttt agtttctgat gaaataacat gtaatcaacc
     2461 agaaaaatct cttttagtta acttaaagaa aaatgctggt agaaacgtac atggtagaat
     2521 aactgttcgt catagaggtg gaggacaaaa aagaaaatat agaataatag attttaaaag
     2581 agataaagat ggtatacctg ctaaggttgc tactatagaa tatgatccaa acagaactgc
     2641 aaatatcgca ttattaaact atgctgatgg agaaaaaaga tatatattag ctccagttgg
     2701 aattaacgtt ggagatacta tattatcagg attaggtgct gatataaaac caggaaactg
     2761 tttagcatta aaagatatgc cagttggtac tatcattcat aatatagaat taaagccagg
     2821 aaaaggagct caattagtta gatctgctgg agtttctgca caattaatgg ctaaagaagg
     2881 aaaaaatgct ttattaagat taccatcagg tgagatgaga ttagtaagca taaactgtaa
     2941 agctactata ggtcaagttg gaaatataga gcatggtaac gtagttatcg gtaaagcagg
     3001 tagaaaaaga catatgggaa taagacctac tgtaagaggt tctgtaatga accctaatga
     3061 ccatccacac ggtggagggg aaggtagatc tccaataggt agaccatctc ctgttactcc
     3121 atggggtaaa ccagctcttg gatacaaaac tagaaagaaa aataaagctt caaataaact
     3181 aatagtatca agaagaacta agtagtaaat ttatttttgc tcggaaagga ggaatataaa
     3241 tgtcaagatc aactaaaaaa gggccttttg tccatgcaag acttttaaag aagatagaag
     3301 ctatgaatgc tagtggaaat aaagaagtta taaaaacttg gtcaagatct tcaactgtat
     3361 ttccacaaat ggtagaaaac actatagctg ttcatgatgg aagaaaacat gttccagtat
     3421 atataacaga agatatggtt ggacataaat taggtgagtt cgttcctact agaactttca
     3481 aaggacataa ggatgacgaa aaatctaata agagaaaata attaaattat gactaagaaa
     3541 ggaggaataa caatggaagc aaaagctact gctaaatacg tacgtgtatc accaagaaaa
     3601 gcaggccaaa tatgtgacct tgttagagga aagaatgttg atgaagcatt agcaatatta
     3661 aagtttactc caagaggagc agcttcaata atagctaagg ttgtaaaatc agctaaagct
     3721 aacgcagaaa acaatcacga aatggatact gaaaaattat atatagcatc aatagttgct
     3781 aatcaaggac caacaatgaa gagattcatg cctagagcta tgggtcgtgc aactacaata
     3841 agaaaaagaa cttctcatat agaggttgtt gttaaagaaa aaaaataatt aatagatagg
     3901 agggaaagta atgggtcaaa aggttaatcc acacggctta agggtcggtg ttataaaaga
     3961 ttgggactca agatggttcg ctactgataa aaaagagttc ggaaacttat tattagaaga
     4021 ccataatata cgtaaattct taaagaaaag attatactca gctggagttg ctaagataga
     4081 aatagaaaga tcagctaaca aaataaaaat ggacttacac gttgctaagc ctggtgtagt
     4141 tataggaaga gctggtgcag gaatcgaagc attaaaagct gaattagaaa aaatgacaaa
     4201 gaaaactata atagttaata tagtagaagt aagaagtaca gataaaaatg ctcaattagt
     4261 agctgagaat atagctttag ctatagagag aagggttgct ttcagaagag caatgaaaca
     4321 agctatacaa agagcaatga aatctggtgc taaagggata aaagtttctg cttcaggaag
     4381 attaggtgga gctgaaatgg ctagaactga aggttatagt gaaggaaatg tgccactaca
     4441 aactttaaga gcagatatag attatggttt cgctgaagct gatacaactt atggaaaaat
     4501 cggtataaaa gtttggatat gtaatggtga agttttacca actagagacg gtgtaaatcc
     4561 aagagaagaa agcagaaaga gcgatagaag agacaacaag agagataata gaagaaacga
     4621 tagaagagga aatgacagaa gaggaaatga taacagagga aactacagag gacaaagacc
     4681 tcaaggtgga tcaagacctc aaagaactga aaacaaagga aattaataat agttaggttc
     4741 aaggaaggag gaaaaatact catgttaatg ccaaaaagag taaagcgtcg tagagttcat
     4801 agaggaagca tggctgggca agctcataag ggtaataaag ttacttatgg tgagtttgga
     4861 ttagtagcat tagaagcttc ttggataact tctaatcaaa tagaagctgc cagaatcgcg
     4921 atgactagat atataaaaag agggggaaag gtttggataa aaatattccc tcataaacca
     4981 gtaacaagaa aaccagcaga gactcgtatg ggtgcaggga aaggttctcc agaatattgg
     5041 gtagctgtag ttaagccagg aagagttatg tttgaattag caggtgtttc tgaagataaa
     5101 gcaagagaag ctatgagact tgctgcacat aaattaccaa tcaaatgtaa atttgttaaa
     5161 aaagaagatt tagaagtaaa gggtggtgaa taggatgaaa gctaaagaat taagagattt
     5221 aacaagcgaa gagctaatga acaaattaaa cgacttcaaa agtgaattat ttagcttaag
     5281 attccaatta gctactggtc aattagaaaa tacagctaga ataaagtttg ttaagaagga
     5341 tatagcaaaa gttaaaactg ttcttgctga aagaaagtta tacgaaacta gagcttaatt
     5401 tggaaaggag gctttttaaa catggaaaga ggaagaagaa aagttagaat aggccgtgtt
     5461 gttagtgata agatggataa aacaatagta gtagctgttg aagaattcgt acgtcatcca
     5521 ctatataata aacgtgttaa gagaactaag aaattcaagg ctcatgatga aaagaatata
     5581 tgtaacatcg gagatagagt taaaataatg gaaactagac cattatctaa agataagaga
     5641 ttcagactag ttgaagttgt tgagaaagtt aagtagtttt tgaagaagga gggattacga
     5701 tatgatacaa caagaatcac gtctaagagt tgctgataac tcaggagcta aagaactttt
     5761 atgtatacgt gttctaggcg gaagtaaaag aagatatggt aacataggcg acgttatagt
     5821 tgctactgtt aaaagtgcaa cacctggtgg agttgtaaaa aaaggtaagg ttgttaaagc
     5881 tgttatagta agaagcaaac aaggcgtaag acgtaatgac ggaagctata tatcttttga
     5941 tgaaaatgct gctgttataa taaaggacga taaaactcca gtaggaactc gtatattcgg
     6001 gcctgttgct agagagttaa gagacaatga attcatgaaa atagtttctc ttgctccaga
     6061 agtactataa ttaaggaggt gcaataggac atgatgcgtg ttaaaaaagg tgacactgtt
     6121 gtagttatag caggtaaaga taaaggtaaa aaaggttcag ttttaaaggt atatcctaaa
     6181 actagcaaag tgttagttga aggtgtaaat gtaataacta aacatcaaaa accaagtgct
     6241 atgaaccaac aaggtggaat aataaataaa gaagccccaa tacacatatc taatgtaatg
     6301 ccatttgatc ctgaaacagg aaaaggcgtt agagtaagat atgaagtaaa agatgggaac
     6361 aaagtaagag tatcagcaaa gagcggaaaa gaattataat aaatattgct tgaaaggagg
     6421 gaccttaaat ggcttctaga ttacaagaaa aatacatgaa agaagttgct cctgctttaa
     6481 tggagaaatt tggatacaaa aacgtaatgg agatacctaa gttaaataaa atagttatta
     6541 acatgggtat aggtgacgct agagaaaatc caaaaggatt agaaaaaggc gttgaagaat
     6601 tagaaatgat atcaggacaa aagcctgtta taactaaagc tagaaaatct gttgctaact
     6661 tcaaattaag agaaggaatg ccaataggaa caaaagttac attaagagct gacaaaatgt
     6721 tctactttat ggacaaatta gtttcagttt ctttaccaag agttagagac tttagaggag
     6781 ttaaccctaa tgcttttgat ggtagaggaa attatgcttt aggagttaaa gaacaattaa
     6841 tattccctga aatagaatat gataaaatag ataaagtaag aggaatggat ataatatttg
     6901 ttactacagc aaaaactgac gaagaagctc gtgaattatt aaaattatta ggaatgccat
     6961 tttctaagta agcaaaggag ggattcccgt ggctagaaaa gcgatggttg ttaagcaaca
     7021 aagaaagcaa aagtacgcta ctagagaata cactagatgt actatatgcg gaagaccgca
     7081 ttcagttttg aaaaaatttg gtatatgccg tatatgcttt agagaattag cttataaagg
     7141 tcaaatacct ggtgttagaa aagcaagctg gtagaagttt gattagaatg gaaggagggc
     7201 aacaaaatga caatgacaga tccaattgca gatatgctta ctcgtataag aaatgctaat
     7261 gttgttaagc atgaaactgt agatgttcca gcttctaata tgaagaaaga attagctaga
     7321 atcttattag aagaaggttt cataagaggt tatgatgtta tagaagatgg aaaacaaggt
     7381 atcataagaa tacaactaaa gtacggacaa gaaggcgaaa gagttataac aggtcttaag
     7441 aaaatatcta agcctggaat gagagtttac gctgctaacc atgagatacc aaaagtatta
     7501 aacggattag gaatatcagt tatatctact tcaaaaggaa tattaactga caagcaagct
     7561 agaaaagaaa atgttggtgg agaagtaatc tgctacgttt ggtaattaat atgaacaatg
     7621 taaggaggtg cgaccatgtc aagaataggt gttaagccaa taataatacc agcaggtgtt
     7681 gaagttacta tagctgaagg aaatttagtt acagtaaaag gtccaaaagg aactttaact
     7741 aaacaattaa gtgctgagtt aaacataaaa aaagaagaaa atacaataat ggttgaaaga
     7801 ccaactgata ataaaaaaca tagatcttta cacggactaa ctagaacttt attagataat
     7861 atggttgtag gtgtaaatac tggtttcgaa aagaaattag aattaaaagg tgttggatat
     7921 agagctcaaa aacaaggaaa gaaattagtt atgaacttag gtttctctca tccagttgaa
     7981 atggaagatc cagaaggaat aactgttgaa gctccaaacc aaacagagtt aatagtaaaa
     8041 ggtatagaca agcaattagt agggaattat gctgctaaga taagagcttg gagaaagcca
     8101 gagccataca aaggtaaagg tataaaatac gttgatgaag taatcagacg taaagaagga
     8161 aaaactggta aaaaataata ccaaaactta aacgctagaa aggagtgagc taggtgttaa
     8221 aaaaggctga taaaaatgct aatagacttc aaagacataa gagagttcgt agaaaaataa
     8281 gcggtacttc tcaaagacca agattatgtg tatttagaag tgctaacaat atatatgctc
     8341 aaataataga tgacacaaaa agagtaactc tagttgctgc atcttcttta gaagcagaag
     8401 ttaagagtgc tgtcaatcat acaggaaaca aggaagcagc taagaaagtt ggagaattag
     8461 ttgctaaaaa agctgttgaa aaaggaatca ctgaagttgt atttgacaga ggtggatact
     8521 tatatcatgg aagaattcaa gaattagctg aaggtgctag agaagctggt cttaagttct
     8581 aatacgaagg agggaaaaat acatgctacg tcgtaagccg atagatgcag gacaacttga
     8641 cttacaagaa aaagttgttg aagtaagacg tgttactaag gttgtaaaag gtggtagaaa
     8701 tttcagattt gcagcattag tagttgttgg agatgaaaac ggacacgttg gtataggtgc
     8761 tgggaaagca atggaagtac cagacgctat aaaaaaagca gttgaagatg ctaagaaaaa
     8821 tctaatagta gtgccaatag ttgggactac aattcctcac gaagtacgcg gacactttgg
     8881 tgctggaaat atattaataa tgcctgctgt agaaggtaca ggagtaatag ctgggggacc
     8941 tgctagagcc gtacttgagc tagctggact aaaagacgtt agagctaaat ctttagggtc
     9001 taacaatcca agaaatatgg taaatgctac aatagaagga cttaattctc taaaaacagt
     9061 tgaagatata gctaaactta gaggtaaaaa agttgaagaa cttctagggt aaggaggtag
     9121 tgtaaaatgg ctaaattaca aataaagtta gttagaagtg ttataggaac tactcctaac
     9181 caaaaaaaga atgttgaagc gttaggatta agaaaaagag aacaagttgt tgtaaaagaa
     9241 gacaatgctc aaacaagagg tatgataaat aaagtaagtc acttattaga agtaactgaa
     9301 atagctgaat aatttttata aagtagtgca aggaggtgca atccatgaag ttacatgagt
     9361 taaaacctgc tgaaggtgca gtaagagcta agagaagatt aggtagaggt actgcaactg
     9421 gacaaggtaa aactgcagga cgtggacaaa aaggtcaatg gtctcgttct ggtggtggag
     9481 taagagtagg atttgaaggt ggacaaatgc cactagctag aagacttcct aagagaggtt
     9541 tcaataacat atttaagaaa gtttacacag aagttaatgt tgaagtttta aacagatttg
     9601 aaaatggaac agaaataact gcagaattat taaaatctac taaaactata agcaaaatag
     9661 gtaaagacgg aattaaaatc ttaggtgaag gtaatttaga gaaagcttta actgttaaag
     9721 ctgctaaatt tacagcttca gctcaagaaa aaatagaaaa agctggtgga aaagcagagt
     9781 tagtataata acctgctaat ctcctagcca gtacaggcgg ggtgaattag cgtgctgtca
     9841 aaattaaaac aagcttggaa gattaaagca gtaagaaaaa aagtaatgta tacacttatg
     9901 atgatcgtga tattcagaat aggtactact ataccagttc caggcataga cactagtatt
     9961 atacaaaaga tggtaggcgg caatagtcta ctttctctgt ataatatgtt tactggtgga
    10021 gcatttagta acttttcact atttgcttta ggtataagtc catatatcac tgcatcaatc
    10081 ataatacaac ttctaacagt aggttttgaa agtttagcag aacttcaaaa atctggtgaa
    10141 gaaggaaaga aaaaaatcaa taagtacact aaatatacag cactagctct agcagttgta
    10201 caagctttgg gaattacatt aggaattgta agaagtgctt taatatcaaa tagtgtgttt
    10261 tttatcacaa ctgttgtaat aacattagtt tcagcaagta tgttagtaat gtggattggg
    10321 gacaaaatta ctgaaaaagg aatcggtaat ggtagttcag taataatatt tgcaggtata
    10381 atatcaagaa tacctacaga tgttattaaa atatcgcaac aagttaaatc tggcgaagtt
    10441 gcaccttggg ttatagtgat tctagctgta gttatattac ttacagtaac aggagttaca
    10501 tttatacaag aagcaactag aaagatacct gtacaatatg ctaaaagagt tgttggaaga
    10561 aaaatgtatg gaggacaaag ttctcatata ccaatgaaag ttaaccagtc aggagttatg
    10621 cctataattt ttgctagttc acttttagct ttccctcaaa caatagctat gtttatgggt
    10681 ccaaatgctc aggcttttgt tcaaaaatac ttaagtatgg caacagaaca aggattttgg
    10741 acatatagat caattgagat tctacttata attttcttct catattttta tacaactgta
    10801 tcatttaata cagaagacat atctaaaaat atgaaaaata atggtggatt tataccaggt
    10861 ataagaccag gtgagcctac tatggattat ttaaatagaa ttttaactag attaacttta
    10921 gctggagcta ctttcttagc aataattgct atggtgccag ctcttacaac acattatatg
    10981 aaagtaaata tgagtctagc tgggacttca ttgctaatcg ttgttggagt tgctttggag
    11041 cttaagagac agttagagtc aaacttagtt atgagaagct atcaaggttt cttaaaataa
    11101 aatggagatg gataatatga gaataatatt acttggacct ccaggtgctg gtaaaggtac
    11161 tcaggcggca ggaatagtag aaaaatacaa tatacctcat atatcaacag gagatatatt
    11221 cagaaagaat ataaaagagg gaacagaact tggaaaaaaa gctaaagaat acatggacca
    11281 aggtttatta gtaccagatg agttaactgt aggtttagtt actgatagaa tatctcaaga
    11341 agattgtaaa aatggattta tgttagatgg atttccaaga aatgtagcac aaggagaaca
    11401 tttagatatc ttcttaaaaa atgctggtat atcactagat aaagttgtca atattgaagt
    11461 tgataagagt atattagtgt ctagagcagt tggtagaaga atatgtaagt cttgtggagc
    11521 tacttaccat gttgagttta atcctcctaa agtagaaggt gtatgtgatg tatgccaagg
    11581 agaattatat caaagagctg atgataatga agaaactgta tctaagagaa tacaagttta
    11641 tctagatgaa actaagcctt tagtagatta ttatagcaaa caaggtataa tagcagatat
    11701 aaaaggtgat caagcaatag ataaagtatt tgaagatatt gtcgcagcct taggaagtgg
    11761 aaaataaatg attatcttga aatcaaaaaa agaaatagaa cttttaagag aagcaggtaa
    11821 aatagttgct gacactcatg aagttttaag aaaagctatt tctccgggaa tatctacttt
    11881 agaattagat aaaatagctg aagagaatat aagaaaatac aatgcagaac cttcttttaa
    11941 aggatatgga ggttttccag gaagtatatg tgcttctatc aatagagagg tagtacatgg
    12001 tattccagga gaaactatat tacaagaagg tgacattgta agtttggata taggagccta
    12061 ttataaagga tatcatggtg attctgcgaa aactcacgga gtaggcatga tatctgaaga
    12121 agataggaaa ttgatagaag taactagaga aagcttctat gaaggaataa aatttgctaa
    12181 attaggatat agactttcag atatctcaca cgcagtacaa acacacgttg agaaacatgg
    12241 tttctcggta gttagagatt tagtgggtca tggagttgga gcaaatcttc atgaagaccc
    12301 acaagtacct aattatggac tcccaggaaa aggaccaaga cttagagaag gaatggttat
    12361 tgctatagag ccaatggtta atgcaggtcg ttatcatgta aagactctat cagatgggtg
    12421 gacgactgtt acaatagatg gaaaaaaatc agctcattat gagcatacga tagcaattac
    12481 tgaacatgaa cctttgatat tgacaaagtt gtaatttaaa aagtaggtga aaaaagtgct
    12541 atcagataat ttaagtatag gtcaagttgt taaagtttca ttaggacgag ataaaggaaa
    12601 tttattcttt gtagtcaaaa taattaataa tgaatatgta ttaatagctg atggaaaaaa
    12661 gagaaaactt gacaaaccta agttaaagaa agtgaagcac ttaaaaaaat ataattttat
    12721 taatgatgaa gttagaaaaa gagtagtatc tggacaagag attacagatt cttttttaag
    12781 agctgaactt actaagttaa attagataat ttaacttatt gaaatggagg gctggttagt
    12841 taatggccaa aaaagatgtt atagaattag aaggtacagt ttcagaagct ttacctaatg
    12901 ctatgttcaa agttaaacta gaaaatggac atgagatatt atgccacatt tctggaaagc
    12961 taagaatgaa cttcataaga attcttgaag gtgacaaggt gaatgttgaa ctttctccat
    13021 atgaccttac aagaggaaga attacttggc gtaagaagta gactatatta atatttagtc
    13081 taaggaggga ttaataatga aggttagacc atcagtaaaa ccaatatgtg aaaaatgtaa
    13141 agtaataaaa agaaaaggaa aagtaatggt tatctgtgaa aatccaaagc ataagcaaaa
    13201 gcaaggataa gaaattatta ctagcatttt gttaaatttt atagtataat actatattgt
    13261 gatgttaaca aaagaacgtt aaaatcgttg aacaatctgg cgtaggattg gcatatgatg
    13321 tgaagtttta aacactaaaa actatgtgta ggaggtgcga tcatggcaag aatagctggg
    13381 gtagatttac ctagagaaaa aagagcggag ataggcttaa cttatatata tggtataggt
    13441 aaagcaactg ctaacgaaat attagctaag gctgagataa atccagatac tagaataaaa
    13501 gatttatcag aagatcaagt taatgagtta agaaaagtta tagatgacga tttcttagtt
    13561 gaaggtgact taagaagaga aattgcttta aacataaaga gattaagaga tataaaatgt
    13621 tatagaggaa taagacacgc taaaggtctt ccattaagag gacaaagaac taagactaat
    13681 gctagaacta gaaaaggtcc tagaaaaaca gtatctcgta aaaagaaaaa ataaataaat
    13741 aataactaga tgaaggaggg aaaaggaaaa tggctaaacc aaaaaagaaa gttacacgta
    13801 ttagaagaag agaacgtaaa aatatagaac gtggtcatgc tcatatacaa tcaactttca
    13861 ataatacaat aataacttta actgacgttc acggaaatgc tatatcttgg gcaagttctg
    13921 gacaattagg attcaaagga tcaagaaaat caactccatt tgcatctcaa atggctgctg
    13981 aaacagctgc aaaagctgcg atggaacacg gactaaaaag tgttgaggta ttcgtaaagg
    14041 ggccaggttc aggaagagaa gctgcaataa gagctttaca agcaactgga ctagaagtaa
    14101 ctatgataaa agacgttact ccaatcccac ataacggatg cagaccacca aagagaagaa
    14161 gagtgtaatt aataagagta caataggagg tgtagataaa tggcaagata tacaggtgca
    14221 tcatgtagac aatgccgtag agagggaatg aaattattcc ttaaaggtga tagatgttat
    14281 acagacaaat gtgctatagt aaaaagaaac tatgctccag gtcaacatgg ccaaggaaga
    14341 aagaaagttt ctaactatgg attacaatta agagaaaaac aaaaagttaa aagaatatat
    14401 ggagttttag agactcaatt tagaaattta tatgaacgtg ctgaaaatat gcctggtaaa
    14461 gcaggtgaga acttattaag tttattagag agaagattag ataatgtagt ttacagaatg
    14521 ggactagcat cttctagaaa agaagctaga caattagtga ctcatggtca tttcacttta
    14581 aatggaaata aagtagacat accttcatta atagttaagg ttggagatgt tatagaagtt
    14641 aaagagaaat caagatcttc tgcaaaattc aaaaatttag tagaagttaa ctcaagaata
    14701 gctcctaagt ggttagaagc taatgtagaa ggaatgacag caaaagtagt tggtgttcca
    14761 acaagagaag atatagatct tgagatagca gagcacttaa tcatagaact ttactctaaa
    14821 taataagcac acttttaaaa attattttaa aattttgttt accctcagtg gattactgaa
    14881 ttttaaggag ggttttgtcc atgatagaaa tagaaaagcc aaaagtagat atagttgagc
    14941 ttagcgaaga ctatagatat ggtaagtttg ttatagagcc tctagaaaga ggatatggaa
    15001 taactatagg taacgcatta agaagaatat tattatcatc attaccaggt gtagcagtga
    15061 atgctataaa aatagatgga gttcttcatg aattttcaac aatacctggt gttaaagaag
    15121 atgttactga gataatatta actttaaaag agctttcagc aactatagat ggtgaaggaa
    15181 gtagaacact taaaatagaa gctcaaggac catgctctat cacaggtgca gatataatct
    15241 gtcctccaga tgttgaaata ttaagtaaag atttagcaat agctacatta gatgataatg
    15301 ctaaacttaa tatggagata tttgtagata aaggtagagg ttatgtttct gctgaagaaa
    15361 ataaaacaga gaatgttcca ataggtgttt tacctgtaga ttcaatatat actcctgttg
    15421 agaaggttag ttatcatgtg gaaaacacaa gagttggtca aaaaacagat tatgataaat
    15481 tagtactaga agtttggact aatggtagta taaatcctca agagggaata tcattagctg
    15541 caaaggtatt agttgagcat ttaaacttat ttatagactt aactgaacat gtaagtagtg
    15601 ttgaaatcat ggtagaaaaa gaagaagatc aaaaagaaaa agttcttgaa atgactatag
    15661 aagaattaga tttatcagtt agatcatata actgtttaaa gagagcggga attaatacag
    15721 ttgaagaatt agctaataaa tctgaggatg atatgatgaa ggttagaaac ctaggtaaga
    15781 agtcattaga agaggtaata caaaaactag aagaacttgg attgggatta aaaccaagtg
    15841 aagaatagcg agcaaaggag ggataagaat ggctaagtac cgtaaattag gacgtgaaac
    15901 agctcacaga aaccttatgt taagaaactt agtaacttgc ttactaagaa gtggaagaat
    15961 agaaactaca gtgactagag cgaaagaaac tcgtagaatg gctgaaaaga tgataactct
    16021 tgctaagaga ggagatcttc atgctagaag acaagtttta gcttatgtta tggatgaaac
    16081 agtagttaat aacttattca cagatttagc tccaaaatat gctgagagaa atggtggata
    16141 cactagaata ataaaaatag ggccaagaaa aggcgatgct gctgaaatgg cttttataga
    16201 attagtatag taaggaaagg attaggcttg atggcttaat ctttttttta tttttttgac
    16261 taaggtatga tataataatg tgtaaaatga tttttataag ttattgctta ctaaaaggag
    16321 tatgagttaa tggataatat agtaaaggta aataatattt cgtttgagta tattacagat
    16381 gaagcaaaac ttaaagcaat agataattta agcctagatg ttaaaaaagg agaatttgtt
    16441 gcgataatag gacataatgg ttctggtaag tctactttat ctaaaaactt aaatgctatt
    16501 cttatgccta ctgaaggaaa tattcttatt gatgatatgg atactaaaga agaagaaaga
    16561 ttatgggata taagacaaac tgcaggcatg gtatttcaaa atccagataa tcaaatagtt
    16621 gctactatag tagaagaaga cgttgcattt ggacctgaga acttaggtat agaaccaaaa
    16681 gaaataagac gaattgtaga ggagtcttta aaaagtgtag gtatgtatga tttaagagat
    16741 agacagcctc atttgttatc aggaggtcaa aagcaaagag ttgcaatagc aggaattatt
    16801 gctatgaggc ctaaatgtat aatatttgat gaagcaacag caatgttaga tccatctgga
    16861 agaaaagaag ttatgaaaac aataaaaaga cttaataagg aagaaaatat aactgttata
    16921 catataacgc actttatgga agaagcagta gaagctgata gagttgtagt tatggaaaaa
    16981 ggaaagaaaa tcttagaggg aactcctaga gaagtattca gtaaaataaa gatgttaaag
    17041 gaaatcggtt tagatgtacc ttgtatgaca gagttatcta gtttattaat agaagaaggt
    17101 ataaatatta gtagtgatat attaactgtg gatgagatgg tgatggaatt atgtcaatta
    17161 tagtaaagaa tttaacacat atatataatg aagggatgcc ttttgcaagt aaggcacttg
    17221 atgatgtttc ttttgagatt aaagatagag attttgttgg tcttataggt catacaggct
    17281 caggaaaatc tacattaata caacatttaa atggattact aaagccttcc tcaggagaaa
    17341 tatttataaa tgattttaat ataacagata aaaacttgaa tttaactgag attagaaaaa
    17401 gagtaggggt tgtatttcaa tatccagaat accaattgtt tgaagaaacg attgataaag
    17461 atatagcttt tggaccctct aatttaggct tagaggagtc tgagatacat aacagggtaa
    17521 aagcatcaat ggaagctgta ggacttgatt atgagggatt taaagacaaa tctccttttg
    17581 aattatctgg gggacaaaag cgtagagtag caattgctgg agttatagcc atgaacccag
    17641 aagttctcat attggatgaa ccaactgctg gtcttgaccc tggtggaaga gatgaaattt
    17701 ttaacttaat aaaagactta catgaaaaaa agaatatgac tataatacta tcatcacata
    17761 gtatggatga tatggcaaag ttagcaaaaa ctttaattgt tatgaatcat ggtagcgtag
    17821 aatttatggg aactcctaga gaagtattta aatcaaatgc tagtaaatta aaagatatag
    17881 gattagatat tccacaagtt ttagagcttg cattgaaatt gagagaaaaa ggctttgaca
    17941 tcagtgagga tatattgact ttagaagaag caaaacagga aatattaaaa gttgtgagag
    18001 gacgaggatt atgttaaaag atataactat agggcaatac tacccaacaa gttctgctat
    18061 tcataaatta gatccaagga taaagcttgt agcgacaatt gtatttatgg tatctatatt
    18121 tgtagtaaat aaattttggc cttatatagt agttttatta tgtttattag ctatgataaa
    18181 attggctaat ataccagtca aatatattgt taagggtgtt aaacctctta aatggattat
    18241 tttgtttaca tttcttataa atattttttt cttaccaggt gatgaaatat ggtcatttgg
    18301 atttttagct attacaaaac agggtctaag acaagcaatt tttatggcaa taagattaat
    18361 atttttagta gttggtacat cattgcttac tttaacaact tcaccaatag aattaactga
    18421 tggaatagag agattgttga atccatttaa gaaaattggt ttgccagttc atgaacttgc
    18481 aatgatgatg acaattgctt tacgttttat tcctacattg ttagatgaga cagacaaaat
    18541 aatgaaggct cagatgtcta gaggtgcaga ttttgaaagt aagaatctta taaatagagc
    18601 aaagaattta gtaccacttt tggtaccttt atttgtaagt gcatttagaa gagctgatga
    18661 attggctatg gccatggagg ccagatgtta cagaggtgga cataatagga ctaagatgag
    18721 agagtctgtt atatcaagaa gggattatat ggcatgtgta tttcaggttg tttatctggg
    18781 agcaattata gctactagat ttatagcaat ttaattaata agttatagat ataatgaggt
    18841 aaagtatgag aaatataaaa attaaaatcc aatacaatgg taagaattat tgtggatggc
    18901 aaaagcaacc tgattcttta gggatacaag gtacaattga aagggctata tatgatataa
    18961 caaaagaaga aaccagtctt atagggtctg gtagaactga ttcaggagtt catgctattg
    19021 gtcagattgc aaattttaaa attaattcag gtataagtat agaaagtata ccaatggcac
    19081 taaatgccaa gttgccaaaa gacatatcag ttatagaagc atgtgaagta aatgatgatt
    19141 ttcactccag atacagtgca aaaggtaaaa catataaata tttagtatat aatagcaagt
    19201 ttagaaatcc aatacttagt gagatatcat atcaagtaaa atatgaactt gattttgata
    19261 aaatgtgttc agaagcaaaa agtttattag gaacacatga ttttaaaggt tttatgagtt
    19321 ctggttcatc tgtaaaagat acagttagaa cgatatatga tattgatata agtaaaaaag
    19381 atgacttgat tacttttgag attagtggta atggtttttt atataacatg gtgaggatta
    19441 ttgttggaac acttgttgat atgggtcgtg gcagaattaa cgaaccattt ttagatataa
    19501 tacaatcaaa aactaggtca agatgtggac atacagctcc agcacaagga ttatttctca
    19561 aaaaagttca ttattaactt gacacaccag gctacatgta ttaaaatata tgagaatgtg
    19621 ttaataagtc cacttgcccc ggacttgaca taaacggtaa aaaaatttta tgaaaagtag
    19681 ttaaggaggg acagttatga aaagttatat agctaagcca gctgatgtac aaagaaaatg
    19741 gtacttagtt gatgctgaag gaaaaacatt aggtcgttta gcaacagaaa ttgcgacagt
    19801 attgagaggt aaacataaac caacatttac tcctcacgtt gatggtggag attttgttgt
    19861 tgtagtaaat gcagaaaaaa tagttttaag tggtaagaaa ttagatcaaa aatattacag
    19921 ataccatact ggttatgtag gtggattaaa agaaatatct tatagagata tgatggataa
    19981 gaaaccagaa gaagttatat cacatgctgt aagcggtatg ttacctaaaa ataaattaag
    20041 aagcagaatg atgactagat taagagtatt tgcaggtgct gagcacactc atgcagctca
    20101 aaatccagaa gttttaaact ttaaataaga ttaataggtg aaaggaggag tttataatgg
    20161 ctaacgttca atattatgga actggaagaa gaaaaagttc tgttgctaga gtaagactag
    20221 ttgcaggtga aggaaatata ttagtaaatg gaagagcatt agaaaattat tttaattatg
    20281 agacattaat aagagatgtt aaacaaccat tagttttaac tggtaatgaa aataaatatg
    20341 atgttatagt aaaagttgaa ggtggaggat tcactggaca agctggagct ataagacatg
    20401 gaatatctag agctttatta aaagctgatt tagatttaag acctgcttta aagaaagaag
    20461 gtttcttaac tagagatgca agaatgaagg aaagaaagaa atacggatta aaagcagcaa
    20521 gaagagctcc acaattctca aaaagataat tacttatttt tttggaaaag caaaatcttt
    20581 tatttactgt tattcagaaa gtattggtat tttaaaaatg ttgggttaag ttaattattg
    20641 atatataata aaataataag atatcttatt aggatgtttt tcattaagaa gaacatcctt
    20701 tttattttga ctatttaata aaaaacatat gctaataaca tttaaatttt atttttataa
    20761 tattgtaatt taaaagttta tataaggtta tatattttaa gatattattt aacaatataa
    20821 attctgaata tacgttatca aagtttataa tataaactaa gtataattta ttgtatagta
    20881 ttgaatgtta gataaacata gtgaattaaa taggggcaga aatataatat cattacataa
    20941 taatatatta ataccaataa ataaagatat atcaatatta aaaaaattta aatgtttgta
    21001 ctaatatatg gaatattatg gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataatttta gatgctggtc atggaggcat tgatccaggt gcattaaata aggataagag
    21241 tacatctgaa aaggatatta acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggc cttgtaatat taactcgaga agatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaaa aatagaaaag aaataatatc
    21421 taattctaat gccaatatgt ttgtctctat acatcttaat gcatttgaac aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga caaacaagac agtaaagcat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtagtaga taagacaaac aacagagaag tcaaaccaag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtactgatag aatgtggttt
    21661 tttatcaaat gaaaaagagt gtaaactctt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatc tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaagttgt tgacttagaa taatatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAMJVI010000063        21623 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.065, whole genome shotgun sequence.
ACCESSION   DAMJVI010000063 DAMJVI010000000
VERSION     DAMJVI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21623
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.065"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(169..456)
                     /locus_tag="PC545_002399"
     CDS             complement(169..456)
                     /locus_tag="PC545_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2457166.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIQKALKQLGIECEVENTDINSARG
                     ADCDCIFTSQELLNELKGTCNVDVYPVKKYMDLAEVTEVVQTFLSDKNDKK"
     gene            complement(469..2388)
                     /locus_tag="PC545_002400"
     CDS             complement(469..2388)
                     /locus_tag="PC545_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2457167.1"
                     /translation="MKERAKKILDFIIKNNNVTSQELQEKFNVSKRTIYYDILAINEQ
                     LGKSGNIKNVKHKFIFEGNLCDARKIISTEEDKFLDSDYRKTFILNKILLGEKISIEK
                     LTNEMLLSKNTVVQTITDVKKYLQTMGLRLEYKGKYKIIGDEYVIRELFLIIVQENVL
                     EINSISEEVSSFDTKGHIKLTDYSLLNLTKFVEFLNKRIRDGKTLYSYKYLNEAKKIS
                     YFSNCKELLCEEANENEQAYICTYISSLPSLNSEVKEDVVEEYVDKLIDKFEVNTAIK
                     LESKHEFKKNILRHLHSSYNRIRFKFPIRNPMLDETKYKHESLYKIIKSIIENEEEFP
                     VFEGIREEEIGFIAAYFGGYLRGSRDNGLRRNKVLLVCPNGLMVSKSLEIQLYKYIPT
                     IEIVGIVSIKQLKEVNVYYDYIITTIDIQNVNNVIVVNPLLTSSDVQLLMNKLISVKE
                     NEKYFNLELIIQAIRKNGVINNEEALKADLLNIIHKIDEGEMYQPMLKELINAERVNI
                     IKNVRDWKEAIKIASKPLLEDNSIEELYIENMIKSVEKYGPYIVLADRFALPHASSKE
                     GVNKLAMSLLIVEDEVDLLGKPVNIFMVLAAVDNTTHIRALASLSEMMYEEENVKLII
                     NGDKSSIIELINKQN"
     gene            complement(2732..3256)
                     /locus_tag="PC545_002401"
     CDS             complement(2732..3256)
                     /locus_tag="PC545_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457168.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLREIL
                     EEVKRDKAELRPKK"
     gene            complement(3260..3757)
                     /gene="coaD"
                     /locus_tag="PC545_002402"
     CDS             complement(3260..3757)
                     /gene="coaD"
                     /locus_tag="PC545_002402"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDF2457169.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            complement(3759..4313)
                     /gene="rsmD"
                     /locus_tag="PC545_002403"
     CDS             complement(3759..4313)
                     /gene="rsmD"
                     /locus_tag="PC545_002403"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDF2457170.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKSCTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            complement(4372..6429)
                     /gene="recG"
                     /locus_tag="PC545_002404"
     CDS             complement(4372..6429)
                     /gene="recG"
                     /locus_tag="PC545_002404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDF2457171.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDGEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKELIIQEYMPQRIIEKYRLCSIDFAVRNIHSPSSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIISALPFKLTKAQNRALDEIIQDMNLEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQIKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGANPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETIAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMRLFKDKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            complement(6510..8153)
                     /locus_tag="PC545_002405"
     CDS             complement(6510..8153)
                     /locus_tag="PC545_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDF2457172.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKELVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVENDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGEFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGDNIEIKNVDLDTNI
                     STSMDFAKSTTSTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKHINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALNSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            complement(8172..8522)
                     /locus_tag="PC545_002406"
     CDS             complement(8172..8522)
                     /locus_tag="PC545_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDF2457173.1"
                     /translation="MSAKVTNQYGNIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            8883..9071
                     /locus_tag="PC545_002407"
     CDS             8883..9071
                     /locus_tag="PC545_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDF2457174.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            complement(9231..9383)
                     /locus_tag="PC545_002408"
     CDS             complement(9231..9383)
                     /locus_tag="PC545_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HDF2457175.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(9481..9957)
                     /locus_tag="PC545_002409"
     CDS             complement(9481..9957)
                     /locus_tag="PC545_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDF2457176.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDIIYKVFSKSGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            10176..10937
                     /locus_tag="PC545_002410"
     CDS             10176..10937
                     /locus_tag="PC545_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2457177.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKEHHNKLLAKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIREKYGKDIIDTSNKKLKNMSK
                     QDYENWQNLNIEIISKLKKAFKTGDASSELAQEVAKLHHKWLSYTWNTYSKEAHSSLA
                     QMYVYDERFTSYYDREQPGLAKFLRDAIVIYTRDK"
     gene            complement(11197..13125)
                     /locus_tag="PC545_002411"
     CDS             complement(11197..13125)
                     /locus_tag="PC545_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2457178.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSING
                     TLEKNGAIIKIKKGEGYYIDILNLALYQQYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQRVVLSEINKFNINFLDFNLK
                     NFIIHLAITVSRILDGYCLDNVLDVVLTDFQSNTTVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRVDTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEITLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQSNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSHIYSNLLDNFFDKNLF
                     IVNPKIKDKDELIKLMCNKLTQSEIVFPSFAESVFYRESLSSTNIDDFLAIPHPMELS
                     SIRTKICISILDEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDNFLSIIKSIV"
     gene            13359..13811
                     /locus_tag="PC545_002412"
     CDS             13359..13811
                     /locus_tag="PC545_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2457179.1"
                     /translation="MVLQEKYIFIDKDLKSKEEVLNFIADSAYILGITTNKEALLKDL
                     LKRENEFPTGIKDGFAIPHTKSVNVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HKNEGNIHLKTLSKLATCLMEDDFTNEVKNASDASHLVKYITNKMKEE"
     gene            13814..14122
                     /locus_tag="PC545_002413"
     CDS             13814..14122
                     /locus_tag="PC545_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2457180.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHEIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGIALKKPEAVLTKCIEAIS
                     "
     gene            14236..15342
                     /locus_tag="PC545_002414"
     CDS             14236..15342
                     /locus_tag="PC545_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2457181.1"
                     /translation="MSKKNKSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPAMGAGFIGGL
                     IASNTNAGFLGAVIAGFFAGYVCKFLKKKIKISGSASGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGAMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSKFSNALAETGKGNIVMGIIGITEGAI
                     PFTLTNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISIGSGWAYLVGAIF
                     GALVIAILSTTLVDFNEDETTENTEFDEIELDIM"
     gene            15417..18095
                     /gene="mngB"
                     /locus_tag="PC545_002415"
     CDS             15417..18095
                     /gene="mngB"
                     /locus_tag="PC545_002415"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDF2457182.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRRRLVIGPWYTQTDQLVISGESIIR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDIIGKLKVKASTNNVYFPNG
                     FDQAPIRKNLPDILKKANEIDNENEYEISDIENYIDSVRNERNEFTELKGEFLNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLVIKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDLHMRLISTQIKNNNEEITLTVFNGF
                     PEVRNEVVEFDTYIPGQDFVIKNKNGKILNYIIKEKEDITNYILTQTIRLNPSKKIYI
                     PKTVYRAKIALSVKDIPPMGYTQLVFELDTKNKCEIETSNCKEIENKYYKITANKNGS
                     LEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNIQEE
                     MILKYSLTIPTSLEERAIGKVSVKMPVTMKITLEPKEQIIKFNVNIKNNQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTVLEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQRGIALITGCVREYQIVGDNLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFDFGLYIYNNKFDEANVANTAKRFLTPIQIYEYADFLNGRLIFAFNDEKQ
                     IYENEFSLMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDAKGSLNINKNVNDAYS
                     AMLDEIYDNTSKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(18412..20745)
                     /locus_tag="PC545_002416"
     CDS             complement(18412..20745)
                     /locus_tag="PC545_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2457183.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYLVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(20720..21436)
                     /locus_tag="PC545_002417"
     CDS             complement(20720..21436)
                     /locus_tag="PC545_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457184.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
BASE COUNT         7415 a         3404 c         2560 g         8244 t
ORIGIN      
        1 gtcacgagtg tgcgtagcta ttttttaatc aagcattata ttttttagtg ataggtcaac
       61 tgtttaagtt ttctaccact aaatccctcc tctctttctt actatttttg aacttagtaa
      121 gtttttattt tattctgatt aacttaaatt tactttttaa aacttatact atttcttatc
      181 atttttatca cttaaaaaag tttgaacaac ttctgtgact tctgctaaat ccatgtactt
      241 tttaactgga tatacatcta cattacaagt tccttttaat tcatttaaca attcttgact
      301 tgtgaatata cagtcacaat cagcacctct agctgaattt atatctgtat tttctacttc
      361 acattctata cctaactgct ttagagcctt ttggattgtc atttttaata ccattgatga
      421 acctacacca aaaccacata ctgctaatat cttcattttt cattccccct aattttgctt
      481 atttataagt tctattattg aacttttatc accattaatt attagtttta cattttcttc
      541 ctcgtacatc atctctgata gtgatgctaa tgctctaatg tgagtagtgt tatctacagc
      601 agctaatacc ataaatatat ttactggttt tcctaataaa tcaacttcat cttctactat
      661 tagtaaagac atagcaagct tgtttactcc ttctttagaa cttgcatgtg gtagagcaaa
      721 tctatcagct aaaacaatat atggtccata tttctcaact gactttatca tattttctat
      781 atataactct tctatagagt tatcttctaa caatggcttt gaggctattt ttattgcttc
      841 tttccaatct ctaacatttt ttattatatt gaccctttct gcattaatta attcctttaa
      901 cattggttga tacatctctc cttcatctat cttgtgtatt atgtttaata aatcagcttt
      961 tagtgcttct tcattattga taactccatt ttttcttata gcttgtatta ttaattcaag
     1021 attgaaatac ttctcgtttt cttttacact tattagtttg ttcataagta attgaacatc
     1081 tgatgatgtt aaaagaggat ttacaacaat tacgttgttt acattttgta tatctatagt
     1141 tgtaattatg taatcgtagt atacatttac ttcttttagt tgcttaatgg atactatacc
     1201 tactatttct atggttggta tgtatttata tagttgtatt tccaaactct ttgataccat
     1261 aagaccatta ggacaaacta gtaagacttt atttcttctt agaccattat ccctagaccc
     1321 tcttaaatat cctccaaaat acgctgctat aaatcctatt tcttcttctc ttatcccctc
     1381 aaatacagga aactcttctt cattctctat aattgatttt ataattttat aaagtgactc
     1441 atgtttatac ttagtctcat ctaacattgg atttctaata ggaaacttaa atcgtattct
     1501 gttgtaagaa gagtggaggt gccttaaaat atttttctta aactcatgct tactttctaa
     1561 ttttattgct gtatttactt caaacttatc tataagttta tctacatatt cttcaacaac
     1621 atcttcctta acttcactat ttaagctggg aagtgaactt atgtaagtgc atatataggc
     1681 ttgttcattt tcattagctt cttcacataa aagttcttta caattactaa agtaactaat
     1741 ttttttagct tcatttagat atttatatga gtataatgtt ttcccatctc ttatcctttt
     1801 atttagaaat tccacaaact tagttagatt taataaagaa taatcagtaa gttttatatg
     1861 tccttttgta tcgaaactac ttacttcctc acttatagaa ttaatttcca gtacattttc
     1921 ttgaacaatt atcagaaaca gctctctaat aacgtattca tcacctataa ttttgtactt
     1981 tcccttatac tctaatctta accccattgt ttgaaggtat ttttttacat cagttattgt
     2041 ttgtactact gtgttttttg aaagtaacat ttcatttgtt agtttttcta ttgatatttt
     2101 ttcgccaagt aatatcttgt ttaatataaa agtttttctg taatcactat ctaaaaattt
     2161 atcttcttca gttgaaatta ttttccttgc atcacataaa ttaccttcaa atataaattt
     2221 gtgttttaca ttttttatat taccactttt tcctagttgt tcatttatag ctagaatatc
     2281 gtaataaata gttctctttg aaacattaaa cttttcttgt agttcttgtg atgttacatt
     2341 gttattttta ataataaaat ctaatatttt tttagccctt tccttcatac tacctcctta
     2401 attttattat ctatattttc tacatgtagt gccataccaa ataattgcac atgatttagt
     2461 attattttat aattttgcac agtctaactc taatctaaaa gaagacagtg gtaaaattat
     2521 taaaaaaatt tgaaaataac ctatattatt tatacaaata aataagttga tttcaacaaa
     2581 taaaagatta aactacatta taatatcaaa ataagatata agtaatctaa aaagcacaat
     2641 tatataacaa aaaattgcta tattaaaaga gagctatcac acataaattg gaattttttg
     2701 tgtgatagct ccatttttat ttatttaaaa tttatttttt aggtctaagt tcagctttat
     2761 ctctttttac ttcttctaat atttctctta aattatactc aacactagaa agcacatcct
     2821 ctgcatattc aattgaccca attcttatct cattggctat tctctcagct ctatcaacta
     2881 tatcttttgc tctttggtct gcttgaacaa caggttcact ggattctaca tatgccttta
     2941 ctacatcttc agaatttttc tttaattcct ctatattatc ttgatattct tgttgtattc
     3001 tctcagcttc ttttttcgct tgttcaacta tatttgtagc atcttgctta gcttgactta
     3061 ttattttatt tctttcttta ttaatccata ctgcctgtgt tacttcttct ggtattaaag
     3121 ttcttatatc attaattatt gctaaaacct catctttatc cactgtgcat ttatgtgaga
     3181 aagggattgt agaagcactt ctaaatatct cctctaactg ctcaaatagt tcaatcattt
     3241 ctaaatctat cttcatatat caattacccc cactagtttt tttctttaat tcttccaaaa
     3301 caatctttgg aaccaaattt tttatatcag catcaaagag caaaacctct tttattaaag
     3361 atgaacttat aaatgaatat tttgtacaac ttggtaaaat tatagtctct atatctggat
     3421 taagctctct attcatatgc gccatctgaa gttcataatc aacatcagca ccacttctca
     3481 cacctctaac taatgctcca atcccatttt cctgacagta atttattaat aaaccatcaa
     3541 aagtcacaac ttttatatta tttaaatggc ttgtactttt ttctataagt tttactcttt
     3601 catcaaagct aaatagaccc tttttattag gattatttaa tactcctatt tgtaattcgt
     3661 caaataactt cgaagccctg caaataatat ctaaatgtcc attagtaatt gggtcaaaac
     3721 ttcctgcaaa tatagctttt ctaggtttat tttccatatt atgcctccat cttataaaaa
     3781 gttaatgtag tatttccata tttcttatct ctagttttct ccaatttaca tatcttatct
     3841 ggaaataaat cattcgtatc atgttctaca actattattc catcttcgtt caatagatta
     3901 gaattatcaa ttttttcaat agcttctata aacatatttt tataatacgg agggtccata
     3961 aaaattatgt caaatttact attttgtaat ttaagtttat ctattgcagt cttaaaatca
     4021 aggtttaaaa taatactttc actttcaact cttgcctttt ttatattgga cttaacaata
     4081 tctatgcttt ccttactgat atctacaaaa gtacatgact tggcacccct agataagcac
     4141 tctattccta aagaaccagt cccagcaaat aaatccagaa cttcactttc cattatatat
     4201 gaatttatca tattaaatag agactctttt actctatctg tagttggcct tacatcttca
     4261 ttttttggag tatttagttt taatcctctc gcttttcctg aaattactct caatatgaca
     4321 atctcccttc agcacattat tacattagta atattttaac acaatttaaa attaatttaa
     4381 tgaaatttct ttcaatgtat ctttaaattt atctataatt tcttttttga gtgccatgtt
     4441 ttcttttaat tgtagattat tatcttcacc caatatatat cgagcttctt gctgtgctaa
     4501 cttcagtatt tttatatgct taaataagtt tgctacctta agttctggaa gaccatgctg
     4561 ccttgtccca aaaaattccc caggtcctct tatttctaaa tccttctctg aaatcttaaa
     4621 accatcattg gtctcttcca ttatagccat tctttgtcta catacatctg ttttagaatc
     4681 ataaattagt acacaatacg atttatgact acctcttcca actcgccctc taagctgatg
     4741 taattgtgca agtccaaatc tttctgcatt ttctattatc attaatgttg catttggcac
     4801 attgacacca acttctatta ctgttgttga aactaaaata tctatttctt tatctttaaa
     4861 aagtctcatt acttcatctt tttcacttga cttcatttta ccatgaagca acccaagcct
     4921 taagtcgtga aaatattccg ctcttaactc ctcaacaagt tcaactgctg atttggcttc
     4981 tatggcttca ctttcttcta ccaagggaca tacaatgtac acttgtcttc ctgattcaac
     5041 ctctcttctt acaaggttat tatatgctct atctcgctta cttttttcaa tagcaattgt
     5101 ttctattggc tgtcttcctg gaggaagttc atctataata gaaatatcta aatctccata
     5161 gagtataagt gctaaagttc ttggtattgg tgttgctgtc attactaata tatcaggatt
     5221 tgctcctttc aatgaaagtt tgctcctttg cataacacca aatctatgtt gttcatctgt
     5281 tataactaaa ccaatattat taaattcaac cttatcttca attagtgcat gtgtacctat
     5341 taatatatca atctcattat tctttatttg ttccaatact gtatcctttt gtttttttgt
     5401 aagactacct attaatagac ctacatttat tccaaaatct tttagagact cagtaagtga
     5461 aatataatgt tgccctgcta atatctctgt aggtgccatc aaagcgccct gataaccatt
     5521 taaaacacaa tttgcaagtg ctaagagagc tactactgtt ttaccactcc ctacatcacc
     5581 ttgtaccaat ctattcatta ttttctctaa attcatatct tgtataattt catctaatgc
     5641 cctgttttga gctttagtca gtttaaatgg taaagcactt attatttttt ttaagtcttt
     5701 acttgtttca aactttattc catcttcttt atttctgcca cttttaaata caaacaaccc
     5761 tagttgtagt ataagtaatt cttcaaatac aattctatat aaagctattt ttaatgattc
     5821 cttactactt ggtgaatgta tgtttctaac tgcaaaatct atactacata gcctatattt
     5881 ttctataatt ctttgtggca tatattcttg tataattaat tctttatctt caagtactgt
     5941 tctaattata ctcataattt ctttatttgt aacaccgtat gtaagctggt acacaggcat
     6001 aaatctacat gtattcttag gtgaattagt aagatattct aattcacatg atgtaagttc
     6061 taagttatta aattttttct taaccttccc aaaaactaat attgaatctc ctggacgaaa
     6121 agtatttttt atataactct tattaaaaaa cactaattta gccgagcctg tctcatcttt
     6181 tacatcaatc tttgttaaag tcataccttc ttttgggcta aatgtattta tacttgatat
     6241 tactgctttt atcgtaactt tctcaccatc ttctaattgt gctattttct ttagattatt
     6301 tctatcttca aattgtcttg gaaaataata caataggtct tttagtgtaa atatacccag
     6361 tttatttaat ttatctgctt ttttaggtcc tattccctta acatattgaa catctttata
     6421 taaatccaac aaatatatca cctactacac aaaaatcctg tacaaccaat tttataaaag
     6481 ctacttggtc atacaggttc ttataatcat tattcaactg aaattaagta ataataaaga
     6541 ggttgtcctc cataatataa ctctacatct acatcttcaa acttttcttc taaagaagta
     6601 cgtagttcat ttgcttgaga ttctgtaaca tcttcaccaa agaataatgt tattatagca
     6661 gtatcttcat caaccaactt ttcaataagg tttgatgtta cttcatccac ataatctcct
     6721 gctgataata aattcccttc tgcaattcca attatattac cttctttaac atcaatttcg
     6781 ttcatcactg tatccctaac agcaaaagtt acttgacctg atttaactga gtttaaagca
     6841 ttcatcatag cttctttatt ttcatcttcg cttaattctc cattaaaagt tactaatgca
     6901 gcaaatcctt gaggcgtatt ttttgtaggt ataactatta tatttttatc actcaattct
     6961 tttgcctgat tagcagccat tattatattg ctgttatttg ggaaaataaa tatattttta
     7021 gcatttatat gctttatagc attcataaaa tcttcagtac ttggattcat agtttgacca
     7081 ccttctatta tatggtcaac tccgaaatct ttaaatatat ttgccaaacc ttcacccata
     7141 gaagtagcta taaacccaaa ctctttttct tcttctaaag gttcactatc attttctttc
     7201 ttttcttcta catccagtaa tgtattttca tgttgtaact tcatattttc tatttttata
     7261 gttaataatt gaccgtactt tagagcttct tgaagtacat ttcctgggtc attagtgtgt
     7321 acatgtacct ttatgactcc atcatctcca actactgcta ggctatcacc ataagccatc
     7381 attatgtctc ttattttagt atcttctacc ttagaacttt ctaaaataaa ttctgtacaa
     7441 tagcaatatt ttatatcatc tgtgctagta gtactctttg caaaatccat acttgttgaa
     7501 atattagtat ctaggtcaac attttttatt tctatgttat cgccttttat agaagctaac
     7561 attccttcat atataagtac aagacccttt ccaccagagt caaccacacc agcttctttt
     7621 agattcttta gaaggtctgg agttctttct aatgaagaat ttgattcctt cacaagcata
     7681 cttaaaaact ttactacatc tttctctttt ttcgcagttt ttattgcaaa ctctccactt
     7741 tctctaacta cagtaagtat agttccttca attggtttta ttacagcttt atatgctgtg
     7801 tctgaaccat ttttaaaagc ttttgctaaa tcttctgttg ataatttact ttttccttct
     7861 atagatttag ctatacctct gattatttgt gaaagtataa ctcctgagtt tcctctagct
     7921 cccattaaag aacctttaga taatgctttt ccgatatctg atatattatc attttcaact
     7981 tttgctaatt cttttaaggc ataagatatt gttaatgaca tattagttcc tgtatctcca
     8041 tctggtacag ggaatacatt taacttgtca actaactctt tattgttttg gagattgttt
     8101 gcacctgaaa taaacatctc ccttaacctt ttaccatcta tatattgaat catttgtttc
     8161 ctcctatgaa attacttaac tcttattcct tgtacattaa tatcaattct agtaactctt
     8221 acgccagtca ttttttcaac tacatatttt actctatcta ttatattatt ggcaacagta
     8281 gatatatttg taccatattg tattataaca taaagttcga ttgctatggc atcttcttct
     8341 acttcctcaa ctcttacacc tttagttgcg ttctcgcctt ttaatagctc tactattcct
     8401 ttagacttat atgctaaacc tactaagcca taactttcca ttgcagctct atatacgact
     8461 tgagctatta cttgattatc tatttctata tttccatatt gattcgttac ctttgcactc
     8521 atggttgacc cccttaagct tatttttatt aaaaacattt cttaatttat tttttactat
     8581 ttttacttat ttattatata aaattataat catatcatac tataaatatg gtataattta
     8641 aacctagaaa agtttttgga taaaatttct tctatagtat atatattcaa agaaaaataa
     8701 taattatttt ccaaatatat ttactttata taaaattata tatataattt tatatgataa
     8761 taagaaattc ttcaatatgt gtaaggcaaa tatatatttc tattgcaaat ttttgttatt
     8821 tatgataata taaatatgtt tttagtcttt aaatagttat cattttaagg aggtgtgcgt
     8881 agatggcaaa agtatgtagc gtatgtggta aaggtaaggt ttctggaaac caagtatcac
     8941 actcaaataa acataataaa agaacatggt cagctaactt aagaagcgta agagcgatta
     9001 tagatggagc tcctaagaga gtaaaagttt gtactagatg tttacgttct ggtaaaattg
     9061 aaagagctta gtaaccacgt ttaaaagcct acttccgtaa gtaggctttt taatttaatc
     9121 atttttactc tttctcataa attttaggat tatacttgaa atccattttg gtaattttat
     9181 tgttactact ttcatcaata ctcaccccac actttatagt taaaactatt ttaacaaaaa
     9241 aataataatg ctccacatcc aataagtatt aatgcggtaa gtgctgacca aatccagcca
     9301 ggaagcaaca ttgacagaat tacagccatt cctgttgcta gacaaagtat tcctagcaat
     9361 accttagact tgttatttct catggaacac ctccacatag aacctgtatc tataaatatt
     9421 ttcatattca agtttatgcg acagttttcc attttagacc aataaacaca ttatgtaatt
     9481 ttaatttttc atactagaat tattactgaa ctccgatata gattctacaa tatccaaaat
     9541 tccttttttt ctacttaacc tttcaggtcc actatataaa acaactggta taactttagg
     9601 aagtttaaaa tttttatctt ctatttcttc aaggcttttt ttcataataa aacttttcca
     9661 aatttgttct ctatacgatt tcaatcttgg gacaatttca gtatccattt ctgtttgaaa
     9721 ttctaacact atataatgtg atactttgcc acttttagaa aataccttat aaattatatc
     9781 aacttctttt cctttatatt cacatataaa agatgtttct aactctatat ttttaagagt
     9841 taattctttt gctattgaga cacttgtaaa attttgaatc aaacttaaaa aactttcttt
     9901 atctgaatac attcttctgt actcttctcg tttttcattg cttcttttta aattcataca
     9961 gttcctccta gactatagac cctctatttt ttagtaaaga tagtctataa attttatttt
    10021 ttaattttca ttagtgagta ttgacagttg tgtgtaaaat atccagtaaa gatatttttt
    10081 tatattttta ttaaaagata taaattttta tattttaatg tattgacatt tacgttgcgt
    10141 aaaagtgtat atttaattta taggaggtac tacttatgga atatacagtt caaaaattaa
    10201 gtaaactagc tggtataagt actagaacac ttagatatta tgatgaaata ggtcttctta
    10261 aaccacttaa aataaattct tcaggttatc gtatatatgg tcaaaatgaa gttaataaac
    10321 ttcaacaaat acttttttat agagaattag gaattaactt agaaaatata aaaaacatta
    10381 taaattcacc aaacttcgat agtttaagtg cgttaaaaga acaccataat aaattactag
    10441 ccaaaagaaa gcaaattgat ttactaatag aaaatgtaac taaaactata gctctgaagg
    10501 agggaaaata cactatgaca gatatagaaa aatttgaagg ttttaaagaa aagatgattg
    10561 atgaaaatga aaaaaattat ggaactgaaa ttagagagaa atatggtaaa gatattatag
    10621 atacatcaaa taaaaaactt aaaaatatgt ctaagcaaga ttatgaaaat tggcaaaacc
    10681 taaatatcga aataattagt aaacttaaaa aagcttttaa aactggtgat gcctctagtg
    10741 aactggctca ggaagttgca aaattacatc ataaatggtt gagttacact tggaacacat
    10801 actctaaaga agctcattcc tctctagcac agatgtatgt atatgatgaa cgttttacat
    10861 catattatga tagagaacaa cctggattag ctaagttttt aagggatgct atagtaatat
    10921 atacaagaga taaataatat aaaataataa aggtggtgtc tcaaaatgaa ctttttagtt
    10981 catgagacac tcttttttgt atgaaatcag atatattttt atcattttat caatgaaaaa
    11041 caggtttctc tctctatttt gagacagaat ctttattgta taataattat aatcaataca
    11101 aaataataac aaattataca aaacttactt atactattta tgctaaaaac aaaaaatatt
    11161 ttattaaagt ttttaaaact tatgtaatat aaaaatctat actatagact tgattatgga
    11221 aagaaaatta tcaaaatcat tacaattaga tatactatct ctaatatcat tgtcatgaat
    11281 tatctttaaa aggatatcat aaatggagtt tatttttata taatcatctt tatttatagc
    11341 caacatcatg attaatttaa cagtagaatc ttcactccaa tatattggtt catccaatat
    11401 ggaaatacaa atcttagttc ttatagagct taattccata ggatgaggta tagctaaaaa
    11461 atcatctata ttggtagaag ataaactttc tctgtaaaat acagattcag caaatgatgg
    11521 aaatacaatt tcactttgag ttaatttgtt gcacatcaac tttataagct catctttatc
    11581 tttgatttta ggattaacaa taaataaatt tttatcaaag aaattgtcca aaagattact
    11641 ataaatatgg gaattattag tgattgactt agatatattc tctatatctt tttttaatag
    11701 ttttaaatcc actaatacaa taggaatact atcatgattt aaaggaattg tcgatattat
    11761 tatatctaca tctgacaatt cactagctaa aaattgatta aatgatagac attgaagtat
    11821 atttatttta tcatgaaaaa ctttgtttaa ttgaacctct aataacctac ttgaaccata
    11881 ccctgaacca catactaata ctacgttttt acacttaata ttactttgaa aaaatctctc
    11941 tattccagca cctatatgca gggatacata tccaatttca tcttctgtta aagagtaaat
    12001 actattttta aaaacttttt ctatagcatt taaagttatt tcaaatgcta aaggataatt
    12061 actctttatt gtatttataa ggggattcac cttatttaaa ttataagatt tagaattgag
    12121 aattgattta aagtgcagta ctaaatcatc aaataataca ctatcatttc taaggtcaaa
    12181 agtatattga ttgttgatta cttctaaaat ttcttcaaca tactctataa ttttagtatc
    12241 taccctattg gaaacgttat ccaaaagaga acttttagtt ataaaatgat tatatagata
    12301 aactctgtct gcctttgaaa taattatagt atatttactc tcaatataat taaatatatt
    12361 ttcaacagtt gtattcgatt gaaaatctgt taaaacaaca tctaaaacat tatctaagca
    12421 gtaaccatca agtattctac ttacagtaat agctaaatgg ataataaagt tttttagatt
    12481 aaaatctagg aaattgatat taaatttatt aatttcactt aacactaccc tttgaagctc
    12541 tattaaatct acattattaa ataattctat ttctctatct gtaattccaa ttatatagtt
    12601 ttcaaaattc ctatttatca tattattaga aatacatctt cgaatatcta tttccttacc
    12661 ttcaacttta actccataat atggcttaga aactaatatt aaattatact ttgacaaaat
    12721 aggctttatt ctttttatgt catttagtat tgtaaattta ctcacataca agctattagc
    12781 taaatcttct agttttatat atgtattgtt atagagaata tattttaaaa tatattgatt
    12841 tctttctata ggagaatctg gtatttcact tgaatccata atatcatcag atattaatgc
    12901 tagatactgt tggtataatg ctaaattaag aatatctata taatatcctt ctcctttttt
    12961 tattttaata atagcaccat ttttctctaa agttccattt atagaagtaa tatcatttct
    13021 tatagttctc tcactcactt ttaaatgatt acttaaagtt ttacaagtaa ttttatcatc
    13081 actttcattt aataatttaa acaacgtgag caatctcttg tgcataaata tcccccctta
    13141 tagaatttct tctaaattat actatttaca ctcatataat acaattactc taataaaatt
    13201 gtattataat aatttatttc cacttctata tggaaattag ttattatcat aatataaatt
    13261 tccatgtcga agtggaaatt agttatattt aatttaatta ggataatgat tacaatataa
    13321 atataagtta agacattatg aatggagtgg aaaaatatat ggtactacaa gaaaaatata
    13381 tatttataga taaagactta aaaagcaaag aagaagtatt aaattttatt gcagatagtg
    13441 cttatatact aggtattaca actaataaag aggctctttt aaaagacctt ctaaaaagag
    13501 aaaatgaatt tccaacagga ataaaagatg gatttgcgat acctcataca aaaagtgtaa
    13561 atgtaaagga agcttccata ctctatatta aaacaactaa cgagttagag tggaaaactt
    13621 tagatgatag taaggttaga tatgtattta gtttgttagc cccacataaa aatgaaggaa
    13681 atatacattt gaaaactcta tctaaattag ccacatgttt aatggaggat gattttacaa
    13741 atgaagttaa aaatgctagt gatgcaagtc atttagtaaa atatataact aataaaatga
    13801 aggaggaata attatgaaaa tagtagcaat aacatcatgt atagcaggat tagcacacac
    13861 acctatggca gcaaaagctt tagaacaagc tgcatcaaaa atgggtcatg aaatcaaagt
    13921 agaacaacaa ggtgctatgg gacgagtaaa tactataaca aaagaagaag cacaagatgc
    13981 ggactttata cttatagcat ctgaccaaac tatatctgga atggaaagat ttgatggtaa
    14041 aaaggtgata aaagtcaaaa taggtatagc gttaaaaaaa ccagaagctg tcttaacaaa
    14101 gtgtatagaa gctatttctt aataaaaaaa tctcactcta atatagaatg agaaaaatca
    14161 aaaattaaaa aattgtctaa tgtaattata acatattatc aaaaaatata acaaacttat
    14221 ataggaggat taaaaatgag taaaaaaaat aaatctcttt taagtgaact taagggacac
    14281 ttaatgacag gtatctcata tatgttgcca cttgtaatag gctcatcttt agttgtagct
    14341 atcccaaagc ttacagcttt agcaatgggt ataacaagtt tagatgctta taaaaatgta
    14401 tctggttttt atcatattct ttacttaatg gaacaagttg gttggactgg cataggtcta
    14461 ttaaatacgg tattagcagg atttatagca ttttcaatag gtgagaaacc agctatgggt
    14521 gcaggtttta ttggtgggct catagcaagt aatactaatg caggattctt aggagcagta
    14581 atagcaggtt tctttgctgg gtatgtttgt aagtttttaa agaaaaaaat taaaatatct
    14641 gggtcagcat caggaatgat gccacttata ataatgccac ttataacagt tggattaact
    14701 ggttttttaa tgagtgttgt attagcagga cctttaggaa acataaatac ttcattaaat
    14761 gcttgggtag ctgaaatgtg tcaaaatgga acaaattcgg tggtattggc tttaatttta
    14821 ggcgcaatga taggattcga tttaggtgga ccagtaaata aagcagcttg gatggcagga
    14881 aatgcattat tgttagaggg aatctatcta ccagcgatat tggtaaactg cgcaatagtt
    14941 atacctccac taggatatgg tttagctaca ttcataagaa aatcaaagtt ttccaatgca
    15001 ttagctgaaa caggaaaagg caatatagtt atgggtataa ttggaataac agaaggtgca
    15061 attcctttca ctttaacaaa tccattaaaa ttagttcctg ttaatatgat aggatgcgct
    15121 ttgggtgtag gattatcagc tttgttaggt gtacatgcta taatgcctcc agttggtgga
    15181 ttatatggat ttatatcaat aggttctgga tgggcttact tagtaggtgc aatatttgga
    15241 gctttagtaa tagcaatatt atcaacaact ttagtagact tcaatgaaga tgaaactacg
    15301 gaaaatacag aatttgatga aatagagtta gatataatgt aaattaaaaa cttaatttta
    15361 gataattgag aattgagtgt actctcaatt ctcaattaaa ttttgggagg atttttatga
    15421 aaaagacaaa agtacatgta gttccacaca gtcattggga tagagaatgg tattttacaa
    15481 catcaaggtc aaaaatatat ttaatgaaag attttcaaga tatattagac atattggaag
    15541 aaaaagaaga ctttaaatac tttactatgg atgcacaagc ttctctctta gatgattatt
    15601 taaaatggag acctcaggat gaatatagaa taagagattt agttcaaaga agaagattag
    15661 ttattggacc ttggtatact caaacagacc aattggttat atcaggagaa tctataatta
    15721 gaaaccttta ctatggaata gatagatgca atgaatttgg tgaacctatg agggttggat
    15781 atgtaccaga ttcttttggg caatcagctc aaatgccaca aatatacaga ggttttggaa
    15841 tagatagttc attattttgg cgtggagttt ctgatgatat ggtagataca acagagttta
    15901 tatggaaagg ttctgatggt tcaaaagtat tagcagttca aataccattt ggttattact
    15961 atggaggtaa tataccagaa gatgattctg atttaaaaga atatttaaaa gatataatcg
    16021 gaaaacttaa ggttaaagct tctactaata acgtatattt tccaaatgga tttgaccaag
    16081 caccaataag aaaaaacctt cctgatatat taaaaaaagc taatgaaata gataatgaaa
    16141 atgaatacga aataagtgat atagaaaact atatagattc agtaagaaat gaaagaaatg
    16201 aatttacaga gcttaaaggt gaatttttaa atgctaaaca tatgagaata cacaaatcca
    16261 tattctcctc aaggtcagac cttaaaataa tgaataacaa attagaaaat tatattacaa
    16321 atgtacttga accattatta acattaagtt attcacttgg aaatgaatac cctcacctag
    16381 ttataaaaga tatatggaaa cttatgtttg aaaatgcagc tcatgacagc ataggttctt
    16441 gtaatagtga cacaacaaat gaggatgtat acacaagata caaacaagcc agagatttga
    16501 gtataaattt attagatttg catatgagac ttataagtac tcaaatcaaa aataataatg
    16561 aagaaataac attaacagta ttcaatggat tcccagaagt cagaaatgaa gttgtagaat
    16621 ttgatactta tattccaggt caagattttg tgataaagaa taaaaatgga aaaattctta
    16681 attatataat aaaagaaaaa gaagatatta caaattatat attaactcaa acaataagat
    16741 taaacccaag taaaaaaata tatataccca aaactgtata tagagcaaaa attgcacttt
    16801 cagtaaaaga tataccacca atgggatata ctcaactagt ttttgaattg gatactaaaa
    16861 ataaatgtga aatagaaaca agtaattgca aggaaataga aaacaaatac tacaaaataa
    16921 cagccaataa aaatggttca ttagaaatat tagataaaga atctaataag acttataaaa
    16981 accaagctgt aattgaagac aatggagatg atggagattc atacaactat tctcctccaa
    17041 gaaaagatat ttatgtttca tctttagatt ctgtatcttc agtagaagtg ctaaaatcaa
    17101 acattcaaga agaaatgata cttaaatatt cattgaccat accaacttca ctggaagaaa
    17161 gggctatagg taaagtttct gtgaaaatgc cagtaacaat gaaaatcacc ttagaaccta
    17221 aagaacaaat aattaagttt aatgttaata taaaaaataa ccaagcacta tctcatagag
    17281 taagagtttt atttaataca gaaatagcat ctaagttttc tatagcagat caacaatttg
    17341 gaataataca aagaccaaca gtattagaaa aagaccttgc attgtggaaa agagacaatt
    17401 actcatggca agaaaaacct ataactatcg aaccaatgca aagttttgtt actttagagg
    17461 atggacaaag aggaatagca ttaataactg gttgtgtaag agaatatcaa atagttggtg
    17521 ataatttaga tacaatagca ttaacactat tcaggtcatt tggatatatg ggaagagaaa
    17581 atttacttta tagaccagga agagcttctg gagaaaaaat aatagcaaca ccagatgcac
    17641 aattacttaa agagttaaac tttgactttg gtctttacat atataacaat aaatttgatg
    17701 aagcaaatgt agctaataca gctaagagat tcttaacacc tatacaaata tatgaatatg
    17761 ctgatttttt aaatggtaga cttatatttg cattcaatga cgaaaaacaa atatatgaaa
    17821 atgaatttag tttgatgaat gtaaataatt caaatttcac agtaagtgca ataaagaaag
    17881 aagaaaaagg agatggaatt gtagttagaa tcttcaatgg tatgaaaaat gaggatgcta
    17941 agggaagtct taacatcaat aaaaatgtca atgatgctta tagtgcaatg ctcgacgaaa
    18001 tctatgataa tactagcaag cttaaagtaa atactaaaac tgtagaggta aattctttat
    18061 cacattgtaa agtacaaaca atagtaatta aataatataa aatagcttat acaagatttc
    18121 aaaataaatt gctagtacta tgttaatagt aaagccagat attatgctat ttataagctc
    18181 tgtaacacta aataacattt tgtaaaataa aaaggtgaac cttgtatatt aggattcacc
    18241 ttttttaaat aaatcaacat tctgttttaa aataacattt ataagctgtt ttgaaataat
    18301 gcttacaaat tattttaaaa caatacctat aaatttttat ttaaagaata cacataaata
    18361 tttccagatt cttttattct atagacttta taattattaa agaagtctaa tctaattttt
    18421 gttaaaagta attgtaactt tgaataaatc accatccaca cttatattca tcttaccacc
    18481 ctgtaattct ataaaacttt ttgctatcgc taaaccaaga ccagaacctt ctgtgttacg
    18541 tgatttatct cctcttacaa atctctccat tatatcatct acactaaaat caatctcaga
    18601 tgcagacata tttttaagca taatatttac tttattatct gtttctaaaa tatcaatata
    18661 cactcttgaa tttggcattg catatttgct tatatttatt ataagatttt caaatactct
    18721 aaatgtacgc tgactatcta atggtaatat tattttttca ttagaaaagt tattctttac
    18781 tattagagat gaatctgcaa gtttgtcatc taattctaaa agtgtctgct tcattagaga
    18841 tacaatatcc acatttacta tattcaaata aacatctcca ctatttgcct tgcttacttc
    18901 aaacaaatct tctattagat gttgcaatct ttgtgacttt ctgtctaatg tatcaatata
    18961 caacttacga ttttcatctg ttatactttc atcttttaat aaatctatat aagtgattat
    19021 agatgttaga ggtgttttta agtcatgtga tacatttgat ataagctctg ttttcatctt
    19081 ttgactctta acctcttcgt caacagcttt tttaaatcca gtttgaatat ttcctaattc
    19141 atcttttata ggattaaaaa atcctaaatc tttttccata tttacatcta agtttccatc
    19201 tgaaattttc tttgtaacat ctaaaagttg attatattca tatattgtct ttttagagta
    19261 atctgacagt atcttaaata atactactaa atatattaat gcgagtatta ttccaaagaa
    19321 ccacatacta cacattataa ctataatcaa taaatttatt cccaaaattg taactatagt
    19381 tttatttggc ttttctttca gatttattgt tctaatttga ttaataactg cttttcctgc
    19441 tttttttaac catacaaaaa acattcctgt cacagtatgt tttgtaaaat actctcttat
    19501 tccaacattt aaaatgtgtt ttaaaagtat tataaataca aatattattg agaatattac
    19561 aaaccaataa gttaaattca ttaaacttac caatatacta gccattttag cacttatgtc
    19621 attgtttata aggtactcta cataattccc actcaaggta ttacttatta taacagtagg
    19681 atataaatct acaagcccct gtataatagc aagagcaata aagagtatct ctaacggtat
    19741 tcttgataat gtatcaaatc ctagcaactc tttagatttt ctatatggga atgctaaagc
    19801 tacaataaca actatagcta atataatttt taaaatcatt ccagaaatct tataataaga
    19861 attattttct acattgctta tatatgaaat tataccatca tcaaaataag tatttccata
    19921 accatactct acactatcac ctttacttat tactttaggt acagcatata ctaatgttat
    19981 attttttatt ggagataact tatatgaaat tgaatttcct cctaaacttg tatccattaa
    20041 atcttctctt gctttagata acaacatttg attaataatt ttttgattta ctccataaga
    20101 atccacaata tttacttttc catctttatc aaaattcatt acaacgtaga atctatagtt
    20161 ttcttttata tctgtaatct gcttttgtct ttcctcatca ttcatttctg tattttttga
    20221 ctgattttca ttagaatttt cttttgaagc atcatttttt gttgtatcaa gtactcctaa
    20281 ttctttatta tcattaaaaa attcaaaatt gctttcttta tcaactaaat agtactttaa
    20341 attctttcct gacgctttaa tgaaatgctc ccaattttca aagttttcat taaaatctgc
    20401 attaatacta tcatctgtat aataagtttc tgaaccatca taagtatctt tttctttact
    20461 tacatttttt atatagttca tagtttcttc attgataaat agctttgatg gttgtatcat
    20521 cttaccttct tgttcttcct tcatattatg atataaaaca taacttcctt tatatatact
    20581 atctacaaag ctttcctgtt caaatggatt ctctgctcca cctttagcac ttgcttgtat
    20641 agtagaatat tgactaatca tacctaatga tgctgataac ataagtataa aaactataat
    20701 agtaactata tatttatctt taaatttttt caattttgta tccaacaccc cacaccacct
    20761 ttaaatattt aggatttttt gggtcaattt ctatcttttc ccttatattc ctcacatgta
    20821 ccataactgt atctgtattt acagcattct cattccaaac tctttcatat atttcatcag
    20881 cagaaaaaac tcttcctgca tttctcataa gtaaacttag tattttaaat tctataggag
    20941 ttgtttttat atactttcca tcaatactaa cttcttttgt attttcattt aattctaatc
    21001 cgcctattgc aaacacacta tcgtcacttt ctaccttttg ctctttattt ccaaccatgt
    21061 ttagatactt agtgtacctt ctaagttgtg aattaactct agccaatagt tctaatggct
    21121 taaaaggttt tgttacataa tcgtctgctc ctatattaag ccccattatt ttgtcaactt
    21181 cttctgattt tgcagatagc attattactg gaaaattata gttttctcta agcttcattg
    21241 taagtgttat accatccatt ttaggcatca ttatatcaac tatagctaaa tgtatttctt
    21301 ggttttctat aaccttaaga ccttctaatc cattgtatcc cttaaaaaca ttatatcctt
    21361 gatttaatag atatatttct atagcatctg ctatctcctt ttcatcttct accaccaaaa
    21421 tattatatga attcattcta atctccttta tattaattta taaattttct ttacatatat
    21481 gattatacaa tatgatagaa gtttttccat ttatatatat atttatatct tttgttctgg
    21541 ttatttgtat ttaatgtcca tcttagaaac ctccttctag tttaaattta atattttttc
    21601 attttgtttt atatacttaa tat
//
LOCUS       DAMJVI010000064        21035 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.066, whole genome shotgun sequence.
ACCESSION   DAMJVI010000064 DAMJVI010000000
VERSION     DAMJVI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.066"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            173..1183
                     /locus_tag="PC545_002418"
     CDS             173..1183
                     /locus_tag="PC545_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2457185.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGAALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGMALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALYDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     VF"
     gene            1294..1782
                     /locus_tag="PC545_002419"
     CDS             1294..1782
                     /locus_tag="PC545_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2457186.1"
                     /translation="MKITVLYQTRSGNTERVAKLIEEGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTIVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FA"
     gene            1958..3886
                     /locus_tag="PC545_002420"
     CDS             1958..3886
                     /locus_tag="PC545_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2457187.1"
                     /translation="MVWHIEYEIFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIDKIPIFITFLLNQIYLLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLIICIILSITNPFTHILFGLSKETGYIRGCGYVATFASFFFYAIYSAFL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLIQIHDVNHVYRIGGDIFSIVVNSQLGIEPDDIIRKIEERFKKPWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKKKHVIKNCL
                     KEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFIPIAEQTGLINS
                     IGLVVFEKVCRFIASDEFEDLKLDNIAVNLSVVQCMQKNLADDLLYLMNKYNVSPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIAYSGVTNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PVPEDVFKNLLKENTFTS"
     gene            4063..4473
                     /locus_tag="PC545_002421"
     CDS             4063..4473
                     /locus_tag="PC545_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDF2457188.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            4495..4797
                     /locus_tag="PC545_002422"
     CDS             4495..4797
                     /locus_tag="PC545_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HDF2457189.1"
                     /translation="MNKKIVAVCESERSLNSLKTAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(5014..5706)
                     /locus_tag="PC545_002423"
     CDS             complement(5014..5706)
                     /locus_tag="PC545_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2457190.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(5732..6919)
                     /locus_tag="PC545_002424"
     CDS             complement(5732..6919)
                     /locus_tag="PC545_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457191.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            7255..9132
                     /locus_tag="PC545_002425"
     CDS             7255..9132
                     /locus_tag="PC545_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2457192.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            9442..10848
                     /locus_tag="PC545_002426"
     CDS             9442..10848
                     /locus_tag="PC545_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2457193.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            10876..11724
                     /locus_tag="PC545_002427"
     CDS             10876..11724
                     /locus_tag="PC545_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2457194.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            12226..12918
                     /locus_tag="PC545_002428"
     CDS             12226..12918
                     /locus_tag="PC545_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457195.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            12915..13850
                     /locus_tag="PC545_002429"
     CDS             12915..13850
                     /locus_tag="PC545_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457196.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            13998..14921
                     /locus_tag="PC545_002430"
     CDS             13998..14921
                     /locus_tag="PC545_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457197.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            14905..15675
                     /locus_tag="PC545_002431"
     CDS             14905..15675
                     /locus_tag="PC545_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457198.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            15685..16467
                     /locus_tag="PC545_002432"
     CDS             15685..16467
                     /locus_tag="PC545_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457199.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            16844..17380
                     /locus_tag="PC545_002433"
     CDS             16844..17380
                     /locus_tag="PC545_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2457200.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            17412..17801
                     /locus_tag="PC545_002434"
     CDS             17412..17801
                     /locus_tag="PC545_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2457201.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            17838..18224
                     /locus_tag="PC545_002435"
     CDS             17838..18224
                     /locus_tag="PC545_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2457202.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            18351..19793
                     /locus_tag="PC545_002436"
     CDS             18351..19793
                     /locus_tag="PC545_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2457203.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            20090..20905
                     /locus_tag="PC545_002437"
     CDS             20090..20905
                     /locus_tag="PC545_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2457204.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         7761 a         2463 c         3872 g         6939 t
ORIGIN      
        1 ccacatattt tgggtattgt atactggaat tagagtaatt aatttatttt ttaacataca
       61 aatgatttta tgaaaaaaaa caagactcaa aaatttttat attctttgat agaataaaat
      121 tgagagtaaa tttattgaat tttataaaat ttataagggg tgagtctaag atatgcatga
      181 agataaaaat gatatatttg aatcaataga tactgaggat tttgataata aaacagaaga
      241 agaatgttta gagctagtaa gaaaaaatgg attgaattta atatacataa aaaatcaaac
      301 agaagagata tgtctagagg ctgttagaca aaattgtaat gcaataaaat gtgttcagaa
      361 tcagacagaa aaaatatgta tagaagcagt taaacaagat tggagaatgt tagaatttgt
      421 agaagagcca acagaagaaa tttgcatgtt ggctattaac caagatggaa cagcattaaa
      481 atatatagaa aatcagacag aggagttatg cttaagagcc gttgaaaaaa atggtgcagc
      541 tttggagtat gtaaaagaac aaacagaaga aatatgtata gaagcagtta gaaacagcga
      601 atttggattg gcaagagtga aaaatcaaac agaaaaaata tgtatggaag cagttaagca
      661 ttgtagctat aatctaaaat gggtagaaaa tcaaacagaa gaaatatgta tggaagcagt
      721 gagacaaaat gggcttgatt tgaaatttgt aaaaaatcaa acagaaacaa tctgtttaag
      781 agcagtaaga caaaatggaa tggcattaga atttgtaaag gaacagactg ttggtatatg
      841 cttaaaggca gtaagacaat atggaatggc attaaaattt gtaaaagaac aaacagaaga
      901 aatttgtaca gaagctataa aacaagataa aagagcactt agttttgtaa aaggagataa
      961 ggaaaaatac aaggctctat atgataataa tgaaccattt gcaaagagat atgttagaaa
     1021 tgtaatagaa aaagaaaaca gagcattgat taaaaaagga gaagaaaatg caaaaatcaa
     1081 aattgcagga aaactttatg atagaggcat gtcttttgaa gatatatcag atatagttga
     1141 gattgaaata agcaagttaa aagtgagttt aggtgttttt taagggaaat agttaataaa
     1201 aaaataatcg actagctctt tgtaggacag caaaattagt gatataattt gatgtataat
     1261 aaattatttt tattagataa gggggaatta aagttgaaga ttacagtatt ataccaaaca
     1321 agaagtggaa acacagaaag ggtcgctaag ctgatagaag agggcgctaa aaaggtggat
     1381 ggagtagatg taaagcttat gagacttgat aatattgatt tagattattt aaatgaaagt
     1441 aaagcagtaa tatttggaac acctacttat ttagcaaata caacttggga agtaaaaaaa
     1501 tggtttgatg aagattcaaa aaaagttaat ttagctggaa aattaggagc tgtttttgct
     1561 acatgtgatt atatatgtgg aggtccagat gttgcgatac ttactatagt aggacatttg
     1621 atggttaaag ggatgcttgt gtattctgga ggtggttctt taggaaagcc atttatacat
     1681 ttaggacatg tacattcaat tgaaggtcca gagttacaag atgagaaggc tataatattt
     1741 ggtgaaagaa ttgctactaa ggcaaaagaa ctatttgctt aatttctata tgcataataa
     1801 tgattagagt aaatttaggg ttttaatgga gatttgaagt aaaagttata aaataaaaaa
     1861 ttttccataa aattactcaa gatgaattat aatataatta taaataaatt atattattaa
     1921 aatatttaaa atcaaacgtc aatagcgagg gaagtttatg gtatggcata tagaatatga
     1981 gattttttct gcaattatag ttattttttt aatggtatac ttttttagaa gtaaatttat
     2041 acctacttta caaaacaaaa tatattgtgc acttttaata ttctcatttt tatttataat
     2101 ttcaaatatt ttaggttcat tttgtctaaa taatatagac aaaattccaa tttttattac
     2161 ttttttattg aatcagatat acttattgtt attgccaata ccagcagcac ttgtatcatt
     2221 ttatgttatg gctattattt atcaagatat aaggtatatg aaaaaaagat tattattttt
     2281 atctatacca cttataattt gtataatctt atctattaca aatcctttta cgcatattct
     2341 atttggattg tcaaaagaaa caggttatat acgaggttgt ggatatgttg caacttttgc
     2401 atcttttttc ttttatgcaa tatattcagc atttttatct tttaaatata gaaaagcaat
     2461 gcatgaatca aaaatatggg ctattagagg ttttcttata gtttcaattg tggcaattat
     2521 cttacaggca atttttttac agtatcttct tacaggaact gcatgtgcat gtagtttact
     2581 tttgacatat ctttctatgc agaatcgagg tcttattatt gatgacctta ctggtgtact
     2641 caatagacaa tcttttattc aagagctaga tttgaatatc aatactgaac aatcaggatt
     2701 tattattaca attgcgcttg atgattttaa gtttatgaat gaaacttttg gaacaaaaaa
     2761 tggagatatt gcactaaaag aagttggaaa atatttgatt cagatacatg atgtaaacca
     2821 tgtatataga atagggggag atatattttc tattgttgtc aatagtcaat taggtataga
     2881 accagatgat attatcagaa agatagaaga aagatttaaa aagccttggc tagtagaaga
     2941 gataagtttt aatttagcaa ctagtattgc tgttgtttat tacccagaaa atgcagaaac
     3001 gacagaagat gtaattgttg ctatagattt ctctatacat gaagcaaaac gttctgagtc
     3061 aaggcgagtt gtttatgcag atgcgtctat aagtgaaaag ataaagaaga aacatgttat
     3121 taaaaattgt ttaaaagaag cattaaaaaa tgatgggttt gaagtgtact accaacctat
     3181 attttctaat agcaggggca ggtttacttc agcagaggca ttacttaggc tagaacacaa
     3241 agagttaggt tttatacctc ctgatgaatt tattccaatt gcagaacaaa caggattgat
     3301 taactcaatt ggattagtag tctttgagaa ggtatgtaga tttatagcta gtgatgagtt
     3361 tgaagattta aaattggata atatagctgt gaatctttct gtagtacaat gtatgcaaaa
     3421 aaacttagct gatgatttac tttatcttat gaataaatat aatgtatcac caactaaatt
     3481 taaacttgaa attactgaga ctgtggctat gggctctttt aatataatta aagaaacaat
     3541 ggaaagatta attgatttag gtgttaagtt tgcacttgat gattttggaa ttgcttattc
     3601 tggagttact aatatgcttt ctttaccatt ttcagtaatt aagttagata agagtttgat
     3661 ttggtctatg aatgaagata gtaggcataa acttacagtt gagacgatta tagctcttat
     3721 aaataaattg aatatgaagg ctgtagcaga aggtgttgaa accattaagt gtgctgaaga
     3781 tttaatagct atggagtgtg aatatttgca agggtattat ttttctaaac cagttccaga
     3841 agatgttttt aaaaatttgt tgaaagaaaa tacttttaca agttaacacc ctttattttt
     3901 ttaattacaa ctttgttcat tgacactgcc agcgatatat tataaaatta acacaataat
     3961 aaatattaaa tttattatac atattattaa tattaattaa tggagtaaag atttattata
     4021 tattttattt aattatccta aagtgagaaa ggaagatgtg acatgaataa aaagaaaata
     4081 ttagtgccaa ttgatgggac agaaagaagt atgcattctt tggaatttat aaaaggaata
     4141 tttaaaaaag atgaagttga agtagaaata atgaatgtaa aagaactagt atttattgat
     4201 ggcatatccc tggctgaaga gataaaaaat tctgaaaatt taggaagaag aatcttggat
     4261 aaagcagcag agataatggg agagtatgat gttaaagtac attttacttt tggatatcca
     4321 ggagatgaga taattagaaa agcaaaagaa gataatgtag attttatagt tatgacaaaa
     4381 tctactaaaa aaggtcttac aagaatgata ggttcagtaa cagcaagtgt agttaaacaa
     4441 gcaaaatgta tagtgatgat tgtaccggag tagaattaga aaggtatttg aaatatgaat
     4501 aaaaagatag tagcagtttg tgaaagtgaa agaagtttaa attctcttaa gacagctgca
     4561 aatgatttag gatttgattt agtttatgaa attcaagaaa atgataatat aaaaaatgaa
     4621 ctttctattt atgatataga agatgcatcc atagtgttat ttgttacaag tgattctata
     4681 gaaagcatag agaagattga tagatttata gatagagaat tttatgaagt tgatccaaag
     4741 tatataatag aggatgcaaa atctatagta agtgaaattt taatagactt aaattaatcg
     4801 tgggtgtcgc actaagaata gagcgacgcc tttttatata ttttacacaa gaaataaaga
     4861 ggttgtatca aaatatagat aagagtcttt ttcattgttg aaatgatgaa aatatatttg
     4921 atttcataca aaaaagagta cctcataact aaaaagttca ctttgagaca ctccattttt
     4981 atatgttagt tgcatttaag gtaaaataga agtttaatca tgttgaaata actgaattac
     5041 atcttctaaa tgattgtaca ttgatgtata acctaagtaa gagtctttac tatcaaatgc
     5101 aatcattatc ttgttatggt ggctaatcat atccttgaga gaatttctat atttaaaacc
     5161 aaagtattgg ttagaagaaa ctgttttagg tattaactca taatatttta taaaaattgg
     5221 attatgagaa gctaaaatta tctctttgtg aaattgcaag tcagcatcat aacattcttc
     5281 tatcaaaccg tgagaaacct tatccaattc ttctattgac tttaggtaag tatattttat
     5341 tttttgaagt tctttagatg tagcatttaa aattgctagt ttagtagctt cactttcgat
     5401 aagttgtctt gctcttaaag attctacata aaattcttgt atgttatgat ttttatagat
     5461 ttcaccatca tcaaataggt caactttttt aaatccttca actataggac ttgaataagg
     5521 cacagctcta agtattcctt ccttacagag tatatttatg gcatttctta caggtattct
     5581 acttatgtta aataggtcag caatctttct ctcacttggt aatttactat taattttaaa
     5641 agttccattt agaatgttta atttaatata atcaataatt ttttcagtgg catttaagct
     5701 tttcataaaa actcctaaac atataatttt tttatgattg tattatatca tagttaagag
     5761 ttaaatctac ttttttataa tatgtataag acaggataga ggccaaaata gctataatag
     5821 ccaagataaa gtatccatat tgtattgaag tatattccga tattttacca actatcattg
     5881 gaaatacaga cattcctata gcataaattg cttgaaaaaa acccattgca gtagatttct
     5941 gatgttttgg tacatttttc atagactcgc tagtaaggta agaaaggagt atacctgtag
     6001 atatacctgg tagtacttgt agtacaaata ttatattaat attttttatt gttggaacta
     6061 aaaagcaata ggctgaaact aaaataaaaa ctaaaggaat ccaaaatttt ggcccatact
     6121 tagtacaaaa tttagtagaa gcaaagccag aagaagcaac agcacacacc atgtaaaata
     6181 tagaagatag acctatgagg gtagaactag aacctaagtt tttaagaatt tgagtagtaa
     6241 atgacattgt tgtagacatt tgaataccct gttgaattaa tgccataaag gaaaaaaata
     6301 caagtctttt atccttgcag atagataaat attttttaat tgatattttt tcaggtattt
     6361 tttttgaatt atgatttttt atatttaagg ctagaatcgt tccaataact gaagacaata
     6421 cactcatgat acataaggct gtcattccta atttttcata aaataaagta ctcataacaa
     6481 atccaatcaa cattcctaga ttgtttacta atattattgt acttgtagct ttttgttgat
     6541 gttctgtaga aaaaaagttg gtatagtgaa ccatgtaaga aatccaagtt gatgatgata
     6601 ttccagataa aatgttagct ataagaaaac cattgcctcc aggagaaaag actcttataa
     6661 gagatgctaa accagcaaag aaaattccag acagtataaa tattttatgt ttatttatgg
     6721 aatcagcaaa tactccaaca ggaagcctta ataaacattg tgagattcca tatgcaccta
     6781 ttattatacc aatcatattt gtagatacat tacatgcact taaatatgga ttttggaaag
     6841 gtatatatac atattgtgaa aaccagaaaa atgtaacaat agaaagtaaa agtaattttt
     6901 gattagatat ttttttcata ttatcctccg tatggtattt ttataatgca aataatatac
     6961 catttggact ttttaatcaa gtactttctc tattaaaaaa aatttaaata tataattatt
     7021 atttacaaaa aaattatact ttatagttga attaagaaaa cgcttatgat actataaagt
     7081 taacaaattt gaatatataa cagtagacgt gttactggta atgcaggcaa gatcgaatca
     7141 atttttatag attatctata gagtactatg ggtataatct atttatttgg ttcgattttt
     7201 ttgtttaatt ggccataaaa caaaaagcct atagttgaaa atggaggatt ttttatggat
     7261 tataaaaata tagctcaaga aattctacta aatgtaggag gaaaagagaa tgtaaatgaa
     7321 gtgactcatt gtatgacaag acttagattt aaagtcaaaa gcgcttctaa agtgaataag
     7381 gataaattat ctaaaactga aggagtaata actgttgttg agagtatggg tcaaatacaa
     7441 gttgtaattg gaaataaagt aaaaaaagta tatgatgagg ttataaaaat tgtacctcaa
     7501 tcaaataacg taggtaaaaa gaatgaaagt caagaaaagc aaggtataat taattctata
     7561 ttatcagcag tagctggtat atttacacct acaatacctg ctatagcagg ggttggtatg
     7621 attaaaggta ttttatcagt tcttgctatg tattatatga ataaaaatgg agtagatata
     7681 aaagaaacgc aaagctatat tatcttaaat gccatggctg attctatatt ctattttatg
     7741 cctattatac taggatatac tgcggctaaa gtatttaatg caaataaaat aatatctatg
     7801 gtattaggag ctacactttg ttatccaact tttactgctt tgatggcagg tgaagaatct
     7861 gttagatttt taggattggc agtaacaaaa gctacgtata catcatctgt tattccaata
     7921 attattgcta tatgggctct ttcatacgtt gaaaaagtat tagaaaagta tatacctgag
     7981 gtaattaaaa taataatggt tccaacttta tcattggtag taatgttacc tgcaacatta
     8041 tttttatttg gaccaatcgg aatttatata ggaaatgtca taaacttttc ttataagtat
     8101 atttatgaat taagcccagc attgtgtgga gcatttgtag gtgggttatg gtgtgtgttg
     8161 gttatatttg gtgctcatag agcattatta ccaataggta taagtgatgt tgctcaaaca
     8221 ggaagacaga atttattggc atttgcagga gctgcaaact tttcacaagc aggggctgct
     8281 ctaggagtat tctttaaaac taaaaatcaa ggactaaaaa caatttctat gtctgcaact
     8341 ataacagctt tatttggtat aactgaacct gcaatatacg gtgccaatct tagattaaaa
     8401 aagcctatgg tatgtgccgt aatatgtggt gcatttggag gtgcgataat gggaatgggt
     8461 ggagcttatg gtaatgcatt tgcaaaccaa ggtgttttaa ctatacctgt atatgctgaa
     8521 gcaggtgcat taggtttctt aagttatcta ggtggttgtg ctatagcatt ttttggttca
     8581 gctatttcaa cttatctagt tggatttgaa gatttggaag agtcttctaa taaggaaaat
     8641 tcatctataa aagtagaaac gaaggatggt gttattgata taacttcacc tgtagagggc
     8701 gaatgtatag aattgtcaga agtaaaggat gatgtatttg cttcaaaagc tatgggagaa
     8761 ggtatagctg tgttacctac aaaaggagta ataacagccc ctactgattg tgaagtagct
     8821 tctttatttc caacattaca tgcaatagga ttaaaattag ataatggagc agaaatgtta
     8881 atacatgttg gaatcaatac agtagagtta aatggtaaat actttaccaa acatgttaat
     8941 caaggagatt tagtgaaaaa aggtgataaa cttatatcgt ttgatataga taaaataaaa
     9001 aaggttggtt atgatgtaac tacgccagtt atagtaaaca atacatttga ttttgggcaa
     9061 gtagtttctt gtaaatctag ttatgtttca accaatgata atataatttc attagtatta
     9121 gctggtaatt aagttaattt gactagagat aagtaaattg atttaaaagg aaataagtaa
     9181 atcaacaatt aaatacatct aaaagtaagt ttattgcaat gaattgttaa agaaaataat
     9241 taatatggag taaatctatt gcaatgaagt aacaaagaaa ataattaata tagagtaagt
     9301 ttattgtaat gaattgttaa agaaaataat taatataggg taaatctatt tcaatgaaat
     9361 aacaaagaaa agagttaata tagagtgaac ttattgcaat gaatcaacaa agaaaataat
     9421 taataaaaag gagaaatatt tatgaataca ggatttccaa aagacttttt atggggagca
     9481 tcatcttctg catttcaagt ggaaggagct tgggataaag acaataaagg aaaaaccgta
     9541 gccgactata attcttttaa aaagtctcat ttacaggcag atactaaagt cgcatcagac
     9601 ttttaccata attatgaaga agatattgaa ttaatgaaag aattagggat gaaaacatat
     9661 cgtttttcaa tttcatgggc aaggataata ccagatggtg aaggagagat aaaccagaaa
     9721 ggtttagact tttataataa gataatagac aaactaatag agtgtgatat tgagccattc
     9781 gtgactttat accattttga ccttccattt aaattagtag agaaatataa tggttgggaa
     9841 agtagagaaa ctgtctatgc ttttgaaaga tttgctaaaa tatgctttaa gcattttgga
     9901 gatagggtaa agtattggca acctcataat gaacagaatt taattgttag agtggaggag
     9961 agaattaata tatatgatga aacagattca tggaagatag ataaaatgcg tgctcagatg
    10021 gattataatc tttgcttagc acatgcatta gcagtcaatg catgtcatga gatgataaaa
    10081 gaaagtaaaa ttggagcagc agtatcatca tctgtaacat atcctcttac ttctaagcca
    10141 gaagatatat atgcagctag aatgaatgat aattttaaag tgtactatat gcttgatatg
    10201 catcattatg gagaatatcc agggtattat atgaagtatc ttgaaaaaag aaatatagtt
    10261 cctcacatgg aagatgggga taaagagata cttaaaaaag ctaaaatgga ttttatagct
    10321 gtaaattatt ataggacaaa ttgtgcagaa gctttacctg aagattcaca acatccattt
    10381 ggattaagag aaggaactgt tgattttagt atgtatggac tatttaaaat gtcaatgaat
    10441 cctaacttag aggcttctga gtatggagca gcaatagacc catcaggtct tagagttgcc
    10501 ttaaatgaat attggcaaag atatcactta cctgttataa taacagaaaa tggtctagga
    10561 gctaaagata tattagaaga tggaaaaatt catgatgact atcgtataga ctatttaaga
    10621 agtcatataa atgcatgtaa actagctatt gaagatggtg tagagatgat tggatattgt
    10681 ccatggtcat ttacagacct tttaagttct tctcaaggtt ttaataagcg ttatggactt
    10741 gtttatataa atagaactga ccatgaaatt ttagatttaa aaagaatcaa gaaagatagt
    10801 ttttactggt ataaagaagt tatagagaac aatggaatag taaaataaac ataaatatat
    10861 attgagggtt ttagtatgta cattacaaag gtattaaata attcgttgct acttgcaaag
    10921 gatgataatg atgaagaaat aatattgatg gggaaaggca ttggccataa ttataaggcc
    10981 gggtctgagt taaatgaaaa agatatagaa aagatttttg ttcttcatga tgaaaatata
    11041 aaaaagagta ttatacaatt ggcaacagaa atagatgcag aatattttgg tatagctcaa
    11101 atgataattg catatggaat agaaaaatat aacttaaaac ttatgaacca tatatatttg
    11161 gccttaactg accacattgc ctttgcagtg agacgtttta aagaaggtat aatgattgaa
    11221 aatcactatt tgttcgaaat caaggaattt aatccaaaag agtatgatat aggaaaatat
    11281 gctattactg tttttaagga ggttcttggt ttagacctac cagaagaaga gattggtaat
    11341 atagcaggcc attttataaa tgcacaacaa gataatcctt atagtgatag aaataagaga
    11401 agtgctaaaa ttgtaaatgc tattttgcaa atagttcatt atcacttttc tattgtatat
    11461 aataaagagt cattttatta taggagattt gtaatgcatc taaaggcctt ttcacaaaga
    11521 tttctggcta atgaaccatc aagagaaaaa atagatttta tatatgagca agttcaaaaa
    11581 aattgtaagg aagagtatga atgtgtgaaa aaggtaggag cttatatgta taaggaatac
    11641 tccagagaat taccaagaca agaggagtta tatttgatga ttcatattca taagatttta
    11701 ggagaattat gcgaaaatag ttagaatatc ttaatatgct agtaatatta taaaaaatta
    11761 aaaattaact gcactttaaa tatgaataat tgcattactt aagggggttg tcttaaaatt
    11821 gattttttta attaaatttg aagctcaact ccttttttaa acctttatat cattacaact
    11881 ttaatgttta tacgcctgta cttcagatgt atatctactt tttacatttc tcaacatgca
    11941 aatctaccat tctgtatata gtaaatgtat tatcatgttt gttttattca agcacactta
    12001 taatacgctt gattttttca caatgatgaa atatactaat gggtgttata ggtataagaa
    12061 tcgcattaca cttgaagaag taagtgataa atgagatata aatcctcata tgataaacta
    12121 ctatttcatt gaggaacata ttctaagtga tgtcaagata ctacaatatg actaaattcc
    12181 tagtgataaa aaagtctacg aatgagtgta gaaaggggaa ctaagatgaa taaagtttta
    12241 attatagatg atgataaaga tctttgtatt ttgttaaaaa agagtatttc aattgaacag
    12301 attgaagcaa actattgcca ttctgggaga gatggactgg ttgaactttc aaaacaagat
    12361 tatcagttag tagttctaga tgtgatgatg ccaggtatgg atggttttga aactatggaa
    12421 caaatacgaa aatatagcag tattcctatc ttaatgctga catcaaaaag tgataattac
    12481 agcaaagtgc atggattgcg agctggtgca gatgactatc taaccaaacc atttgaaatt
    12541 acagaattta ctgctcgtgt actgtcttta atccgacgtt acacccgttt taatccaaaa
    12601 aatgaggagc cacagcaact ttctttcaaa ggtttgttga ttgaccttga ccagcataca
    12661 gttactacca atggcgaacc tatgttgctt ccacccaagg aatttggtgt acttctttac
    12721 tgtgcaaggc atcagggtaa gattttgaca aaacagcaga tttatgaagc cgtgtggggt
    12781 gagccatatg tatatgatga cagcaatatc atggctatta tcagtcgcct aagaaaaaaa
    12841 attgaactag acccatcatc tcccatctat ttgcaaacaa taaaaggaat tgggtatcaa
    12901 ttcaacacgg aggtatgaaa atggatgaac aaacgataat tcttttctta ctcttgctgt
    12961 cgattggtct actttttctt tccatatttg ctttttccaa gcttgggcat attcacaaac
    13021 gcttaaaaga tatagaggag atactcgctg atgtacaaat tggagacgaa aatcgtaaaa
    13081 tactaattaa gccatgtgat gttatggctc cattggttta tcagcttaat gagattgtct
    13141 acgattatga aaataagtta ctttctctga aaaagtcgga taaagctagc aaacaactta
    13201 tgacaagttt atctcatgat gttcgtacac cattaacaac tctgattggt tatctggatg
    13261 ctgtacacag tggtatagtt atcgaacaag agcgggagga ataccttgaa atcgctaggc
    13321 gtagagccta cgacctcaag gattacattg atgttctttt tgactggttt cggttgaact
    13381 ctgatgaatt tacgctgtca atagaatctg tggaaattgc agagttgtca cgcaatattt
    13441 tgaaagactg gattcctata ttccatgaaa agaaactgaa ttttgaaatt gatattccag
    13501 aaaatcgact aatggtaaac ctcgacccag atggatattc ccgtgtggtc aataatctgg
    13561 ttcaaaatgt tcttgctcac agtaaggctc ggcaaatcaa gattaccatg agtgaggatt
    13621 ccagaatggt attactccgt gttgaggatg atggtgttgg gattgccaga gaaaatttac
    13681 cacatatttt tgagcgactg tataagtgtg ataaaggacg ttcagaaaaa ggaagtggtc
    13741 ttggtctatc tattgtcagt caaatggtgg agcgaatggg tggtcaggtt tccgtagaaa
    13801 gtgagattgg aaagtatacc gtatttacag tgagcttacc attaatataa aattgttttt
    13861 cccccatgtg acatgtatac acagggggat tttttgtaag ttataaagtt gcaaggttaa
    13921 tacaaggttg ttgcaaggtt aatgtaagat tgaggtggta tgataacttt aataaacaaa
    13981 ggaggctttt attatttatg aataatttaa ttattgaaac aaaaaatctc actaagcaat
    14041 atggtgctca aaagagcgtt tctaatttga atattcacgt tcagaaaggt cgcatttacg
    14101 gattgctagg aagaaacggt gcgggtaaga cgacaactat gaaaatgtta ttgggcctaa
    14161 caaaaccaac tactggagaa attaaaatat ttggtcagga cattcacaat aacgagaaga
    14221 agcttcttcc tcgaattgga tgtttgattg aatcaccagg gttttatcct aatctgacag
    14281 gaacagaaaa tctcaaaatc tttgcaaggc ttagaggaat accaaaaaaa aatgcagtag
    14341 aatcagcact ggatgtagtc ggtttgccaa acaatgacaa aaagttattt tctcaatact
    14401 ctcttggtat gaaacagagg ttggcgattg ctcttgccat tatgcatgac ccacaactgt
    14461 tgattttgga cgagcctatc aatggacttg atcctattgg tattgcagaa gtaagaacgt
    14521 ttattcgtga tctctgcaca gaacgaggta aaactatttt gatttccagt cacattcttt
    14581 ctgaaattgc cttgttggca gatgacattg gcattattga ccatggtatt ttgttggagg
    14641 aagaaagtct tgcccaatta gaagaaaaaa acagtaaata catacatttt gctgtatctg
    14701 atgcagcaca ggcagcaagg cttctggaaa cccaacttaa cctgaaaaat ttcaaagtag
    14761 cagataatta caatattatg ctatatgata cagatgtttc tgttgcagaa ataaatcgtg
    14821 ctttcattct aaacaacatt gaggtatcag aatctcattt atgcgaagat actcttgaag
    14881 attacttcaa gaaagtaaca gggggtgaag gaattgcttg atataatttc atgtgaattt
    14941 tcaaaactca agcggttaaa atttatactg atttctattt taggtgcttg cttatttcca
    15001 attcccgcaa caattttgat tgcaaaggac aacttgccct ttgagcaatt gttcaaatta
    15061 gttgtcaatt ttggatattt cttgctatta ccaattgtat taagtattgt tgctagccaa
    15121 ctatttttta tagaaagaga taatgatgta ttaaaaaatc ttgcaacagt gccagtgcca
    15181 aaaggaaaac tggctctggc aaaattggct gtgttgctgt ttatatcatt gttctactct
    15241 gttgcagggt taggagctac tatcattgga ggtttcattg ttggaatagt agagggggtt
    15301 gctgtaaaat taggaatgag cattgctttt ggaattatgt tttttgttac tgtacttcct
    15361 gttgtggtac tcattgtata tttcaatcgc agctatattt tttctattat actttctttt
    15421 gtatatgcta ttttcaattt tagtatatcc ttaaatatta tcaattttga accaaataac
    15481 ccactattaa gcgtattacc tgctcctgtt ataatgcgtt ggtggatggc attttgggga
    15541 gatcccactg gcgaatacac ggtacttaga cagccatatt tgctctcaac tcctgcttgt
    15601 gtaggtattt tatgtttgat aaccattata gcagtcctgc tgatttgtat gatatacaaa
    15661 aagcaggaga attaggaggt atctatgctt gatataatta ctacagaatt tcaaaaaata
    15721 aaaagatata atattctatg gattggtgtt gttgctgttc tgttttcagc actattggca
    15781 gtttttcagg ttgtaagttc acacggcagt gaccctctaa cgtatgagag ctttgcaaac
    15841 ggagttatct ggaataattt cagtcttagt tttccatttg ggattacact tattggagga
    15901 tatcttatta accgtgaata tacagaccaa accttaaaaa acatcctaac tgttcctatt
    15961 tcattacgca aattgctagt gggaaaatta attgcggttg gaggaattac tgtattgtta
    16021 agtcttttca gcttcttatg cacatttatt ttggggttgt tattttgcaa tatcaatata
    16081 tctgttatac tcgttgtaaa atccttagtt cagattttgt ctgttaatct gtattgttat
    16141 gttgctgttt tgccgattat tgcttatttt ggtcgtaaac aaaatgcatt tctgacgggt
    16201 gtagggattg catttgtata cggtttttgt ggtgtgtttg ttgctggaag aaatctgaca
    16261 gacttttatc caatcacagc aggtcttggt ctagttggat acaacaatgg tgctggtact
    16321 gtttatcagc cacttatcgg tgctatgact ttgattgtaa ttctaatctt gacaactatt
    16381 ctacttgtat ttacacctaa ttatgataaa gttatgacca tatctaagaa aaaagggata
    16441 aagcataaaa agtattctca taaataacat aaatattgtt tctattattt aagttgctag
    16501 acagtgaaaa gaaaaaatct gtcgtacagt ggctatatgc tcatgtattc aagaagctac
    16561 agttttgatt tttcataact gtagcttctt tttgtattta ttaaatcacg actatcataa
    16621 tacttgcaat aggtatagta acaagagaga gtactgtcat atgtgtttta tatattacat
    16681 taagtatatt ctaaaatata tgttttaaat aatatatttg aaaataggaa tatttaaata
    16741 aaaaaaataa tattatatat agttgacaaa aattggcaaa tgatatacta tataaatata
    16801 gaaatagtaa tcattactat tattaaaaag ggaggaatta attatgaact taaaaggaac
    16861 taaaacagaa aagaatttaa tggcaggatt tgcaggagag tcagaagcaa gaaataaata
    16921 tacatactac gcttcaaaag caagaaaaga aggttataat caaatagctg ctatatttga
    16981 agaaactgct aacaatgaaa aggaacatgc taaattatgg tttaaacttt tacatgatgg
    17041 tatgccttca acagaggaaa atttaaagga tgcagcagca ggtgaaaact atgaatggac
    17101 tgatatgtat gcaaaatttg ctaaagaagc tagagaagaa ggatttgaca agatagctta
    17161 tttatttgaa gctgttggaa aaatagagaa agagcatgaa gaaagatact taaagctttt
    17221 agaaaactta aatgaaggaa aaatatttaa aagagatgaa gaagttgttt ggcaatgtca
    17281 aaactgtggt catgtgtatg taggtactga agcaccagaa aaatgcccag tatgtgacca
    17341 tccaaaagca tattttaata taaaagctga aaattattaa aaattatcat tattaaaaag
    17401 gaatgttaac tatgaaattt tctaaacaac gagaactgat tttaaatgaa atattaaata
    17461 atccggttca tcctactgcg gattacctat atgaaaactt aaaaaaagat aatccgaatt
    17521 taagcttagg aactgtgtat agaaatttag ctcagctaac ggaacatggc tttataagaa
    17581 aggttagtat tccagggtat ccagatagat ttgatggtcg aatagataat cactatcata
    17641 tcatatgtga agtatgtgga gaggtatacg atttagaatc cgaagttctt aataatttac
    17701 aagaattaat atctgaggag acagacataa aaataacatc ttataacata agttttaaag
    17761 ggatttgtaa taattgtaaa aggtgtagcc aagtaggtta gttttcaaat tctaaaaata
    17821 attaatgagg tgagtttatg tgtagtgaac aaaaattttt tatatgtgag acttgtggaa
    17881 accttgtagg aatgatacaa agtggtggtg tacctatttt ctgttgtgga aaacctatga
    17941 aggaacttgt tcctaataca acagatgcag cagttgaaaa acatgtacct gtaattgaag
    18001 tggatggaaa taatgttact gttaaagtaa gtagcacaac tcatccaatg actaaagaac
    18061 atcatatagc atgggtgtat cttatgactg aacaaggtgg tcaacgcaaa tgtctagcag
    18121 tagatggaga acctgtagtt aaatttgctt tgaatgatga tgataaagtg atttctgcct
    18181 atgcttactg taatttacat ggattatgga aggctgaact gtaatattta aataaattga
    18241 gtcaaaaagg aggctataaa tgtattttca tacaaatagc ctcctataaa tttatataaa
    18301 ttcaagtttt aattttgttt aatgtcaata taatataagg agaaaataat atgtcaattt
    18361 ataaatgtag tgtttgtgga tatatttatg atgaatctaa gaatgataaa acatgggatg
    18421 aattaagtga agattgggaa tgccctgtat gtacaaaagg tcgtagttat tttggaaaga
    18481 taagtactgt ttactatgaa gaagatgaaa agatagcaga agacatagtt gaagatgaat
    18541 ctaaattaaa tactgaaaaa gagggagatt taaattactt aagtacatat ttaagaagag
    18601 atgatgaagt tgagaaacat atggatataa ttcacgaaat ggctgttaca ggaaaatcta
    18661 taatagaacc tatgagaact aaactaccag taatttcttg ggatgatatt ttaattatgg
    18721 gtgctcaatt aaatccatta cctttaaatg agcatgatga agtaaataca acaacaataa
    18781 taggaaaaaa agcaaagaaa cctatgatta tagaaaatcc agtttatatt tcccacatgt
    18841 catttggagc tttgtctaaa gaactaaaaa tagctttagc aaagggtgca gcccaaaata
    18901 agacagctat gtgtagtggt gaaggaggta tattaccaga agagaaggaa gcttcataca
    18961 agtatatttt tgagtatgta ccaaataaat atagtgtaac tgaagaaaat cttaaaaatt
    19021 ctgatgctat agagataaaa attggtcaag gaacaaaacc tggtatggga ggtcatcttc
    19081 ctggcgaaaa agtaactgaa gaaatagcaa aagtaagaaa taagcctgta ggtcaagatg
    19141 taataagtcc atcttgtttt gaggaaatac aatccaaaga ggatttaaag aaacttgtag
    19201 atgaacttag agaagtttca gaagggcgtc caataggtgt taaaatttca gcaggtcata
    19261 ttgaaaaaga tatggaattt atagcttatg ctaaacctga ttttgtaact attgatggtc
    19321 gtggtggagc tactggagca agtccaaagt tgcttaaaga tgcaacttca attccaacaa
    19381 tatttgctct ttatcgtgct agaaaataca tagatacaca tggattagat atagatttgg
    19441 ttataacagg tgggcttcgt atatctactg attttgctaa ggcaattgct atgggggctg
    19501 atgcagttgc tattgcaagt tctgctctta tggcagcggc gtgtcaacaa tatcgtatat
    19561 gtggttctgg taagtgtcct gtaggagtag ctactcagga tgaggaattg agaaaaagat
    19621 tacatataga aaatagtgct aatagagtgg caaacttttt aaatgtaagt ttagaagaac
    19681 ttaagacttt tgctagaatt tctggtcata aagatataca tgatttaagt gttgatgatt
    19741 tatatactgt taactctgaa atatcaaatt atacaaacat acagcatgta tagattgaag
    19801 tttgaaacta ttagatttta gacagctata aaagtaaatg ataaatatat tttataaaag
    19861 agagtgtctt aaaatgaact ttttagttca taagtcactc ttttttgtat gaagccaaat
    19921 atatttatca ttttagcaat gaaaaagaat cttatctctt tattttgaga tagactcctt
    19981 ctatttatga aaattaaaga tattaaattg taaaatattc tgtgatgtcg tactattaaa
    20041 ctaaagattc tgtactttaa agtaaataaa aatttaagga gtgattatta tggaaagatt
    20101 gaagatacaa gttttagtcg acaataatac ttatattgat agatactttg ttggagaacc
    20161 agcagtaagc tattatattg agattgatgg aaataggatt ttgtttgact cagggtattc
    20221 agatgtattc atatcaaacg ctgaaaaatt aaatatagat ttaggaaatt taactcatgt
    20281 tgttttttct catgggcata atgaccatac aagaggtatc cagtttttag aaaataggta
    20341 tgatttatct acagttgaac ttatttcaca tcctaactgt tttataccta gaaaacatgg
    20401 agagaaaagc ataggagctc cattttctgc tgaggaaatt aaaaatattt ttatgtataa
    20461 tcctaaggat aagccattta atctaagcaa gaattgtgtg tttttaggtg aaataccttc
    20521 tataaatgat tttgaaaaaa gagctaaaat tggtaaatgt aaaataggag atttgtggga
    20581 agatgattat gttttagatg attctgctat agtttgtaaa actgataaag gtatttttat
    20641 tgttacagga tgttctcata gtggaatttg taatatagta gagtatgcta aaaaggtatg
    20701 tggagatgat agagttattg gtatactagg tggttttcat ctttttgaat tgaataatag
    20761 gttagatagt acaatacaat atttggataa agaagaaata agaatgttgt acccttgtca
    20821 ctgtgtttct tttaaggtaa aagcgaagat gaatgaaata ttgaatataa atgaagttgg
    20881 agtagggctg accataagta tatagtattt gaaagtatat aagatttaag ttgaaggtta
    20941 aaaagatatt ttctatagaa aatatctttt ttgttacgtt tattttaaga gaaatcaata
    21001 tacttttata tgaaatattg taaaaaaatt tattt
//
LOCUS       DAMJVI010000065        20747 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.067, whole genome shotgun sequence.
ACCESSION   DAMJVI010000065 DAMJVI010000000
VERSION     DAMJVI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20747
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.067"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(31..873)
                     /locus_tag="PC545_002438"
     CDS             complement(31..873)
                     /locus_tag="PC545_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2457205.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKSK
                     IERTFVIQDESFLDKISKLASQVDEKFFEVSTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENITIKNDLLHEIKRIHKKEYEIGKWAVDYINKEFDVKFPVDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     KEKINDTNNEIIDIIKVKYEKDYDCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1134..3176)
                     /locus_tag="PC545_002439"
     CDS             complement(1134..3176)
                     /locus_tag="PC545_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDF2457206.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWSNIPLAIYQIVLGIILSILP
                     FKFSFSFNPEIFVICIIAPLLFSEGQNVSRKELLELRKPILLLAFGLVLITVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNIMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFMITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFVYFVAEEIGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNENMALELTFISTLIVLILFVIRFIWVYLWH
                     NSFIKPKKNPLNNFFVGFLGFKDEEVIKESISKCKYSLIVATCGVHGTFTLATALSIP
                     FYLADKDVFPMRDTVLFISSEVILISLVLATILLPRLLKNNVQESEPLLSDKEAYKLI
                     LKEAILKLSNEKAQEVQPVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADQQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIVKLKISMWIINRKVKKEVKEKIT
                     KIVSENKEIIKEFHHAYALSTKTAISFINKNTDEDNRHEALIAIRFYNRYLNWITRKI
                     QNDDKFEARVEYYRVMAIQSQRDSVQQLVETKQISREVAKELLENILYDEMMMIES"
     gene            complement(3328..4299)
                     /locus_tag="PC545_002440"
     CDS             complement(3328..4299)
                     /locus_tag="PC545_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDF2457207.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFDKKIKSVKAVDGISFDLKKG
                     ETLAIVGESGCGKSTAGRTILRLIEKTEGKVEFNGVDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHNLVPKNEIKDNILNVVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKGELYLHSQHPYTKALMSAVPIQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEIPVLKEISRNHFVACHLV"
     gene            complement(4300..5277)
                     /locus_tag="PC545_002441"
     CDS             complement(4300..5277)
                     /locus_tag="PC545_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457208.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNEVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFRGKDILKMNEDELMGIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMIHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVLVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPALGKNKDKRLYSIPGQV
                     PNPIGMPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5498..7057
                     /locus_tag="PC545_002442"
     CDS             5498..7057
                     /locus_tag="PC545_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2457209.1"
                     /translation="MKFKKLASLILVSSLMLTFTACASNDKDKSSGSKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNAD
                     YFGGEPKADSIALKIIPNEASQETALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQDTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAEIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIKDAKPSPAI
                     LFEDWSKLYVE"
     gene            7163..8122
                     /locus_tag="PC545_002443"
     CDS             7163..8122
                     /locus_tag="PC545_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457210.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     SQDIENIRVNMGLDKPVYIRYLNWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKVSSVFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVITAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8125..8937
                     /locus_tag="PC545_002444"
     CDS             8125..8937
                     /locus_tag="PC545_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457211.1"
                     /translation="MFTSILKKLKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLIDMLMSIPSFLVLIVLNTYLTPKVSTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQRHIFDKLYLAVFPGLLIFLTVMSLTIISKKFSSNT"
     gene            complement(9153..10055)
                     /locus_tag="PC545_002445"
     CDS             complement(9153..10055)
                     /locus_tag="PC545_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457212.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKVRIGIPPL
                     ILGIAFSDVVAQLVANNPDIEFDIVEKGAYDLNRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNHPITWKVILCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10410..11588
                     /locus_tag="PC545_002446"
     CDS             10410..11588
                     /locus_tag="PC545_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDF2457213.1"
                     /translation="MKDVVIVSAVRTPIGSFGGVFKNTSAVQLGTITVKEAISRVGLN
                     LSEIDEVIIGNVLQTGLGQNVARQIAINAGIPNSVPSYTVNKLCGSGLKSVQLAAQSI
                     TSGENDVVIAGGTENMSQAPYIVPTARFGSKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQSKAENAIKNNRFKEEIVPVDVLIRRGKIETIDKDE
                     YPKLGMTFEGLAKLKPAFKKDGTVTAGNASGINDGAAMLILMSQQKADELGIRPLAKI
                     KSYASAGVEPEVMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVETGLATLCIGGGQGISMVVSR
                     "
     gene            11597..12253
                     /locus_tag="PC545_002447"
     CDS             11597..12253
                     /locus_tag="PC545_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HDF2457214.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKITIVEADEIVEIGDIDPNEVNTPGIFVNYIIEGGNL
                     "
     gene            12253..12918
                     /locus_tag="PC545_002448"
     CDS             12253..12918
                     /locus_tag="PC545_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HDF2457215.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDNVNV
                     ILQSENGFLGLGSAEDGNSSDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISATEAPLILSDSLNGKI
                     SVV"
     gene            13023..13799
                     /locus_tag="PC545_002449"
     CDS             13023..13799
                     /locus_tag="PC545_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HDF2457216.1"
                     /translation="MVKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNNT
                     ITELQKKGYDCMSVKCDVTKEEEINHAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13861..15195
                     /locus_tag="PC545_002450"
     CDS             13861..15195
                     /locus_tag="PC545_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2457217.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVILNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSIVGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLDRELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15691..16587
                     /locus_tag="PC545_002451"
     CDS             15691..16587
                     /locus_tag="PC545_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HDF2457218.1"
                     /translation="MMRRNIPTKLKTILALGVLTLATASSAVTASASSLENNVNISNK
                     SLVYSNTKYKFSAKPNCSVKPELKPDKDNSCKDKNHNDKNCNNTNKPEDNNNSDSTNK
                     PGNNKPESNKPEDNNNSGSTNKPDNNKPESNKPEDNNNSGSTDKPEDNNNSDSTNKPD
                     DNNNSGSTSESFSAYQKEVVDLVNIERAKAGLNPLTLDSSISNVATKKSQDMIDNNYF
                     SHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVVNAWMNSEGHRKNIMNPNFS
                     KIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(16979..20518)
                     /gene="nifJ"
                     /locus_tag="PC545_002452"
     CDS             complement(16979..20518)
                     /gene="nifJ"
                     /locus_tag="PC545_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDF2457219.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLIAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     GIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGITMSHLRFGDTPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHSCIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRMQL
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKGDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVIEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7160 a         3248 c         2920 g         7419 t
ORIGIN      
        1 tctatacatt ctgtattaaa ctactcttta ttataaattc agtacagata taactctttt
       61 gatatgtaat gttaaataaa gtagttcatc ttgacttaca tagtaatcat aattcttttc
      121 tacatgagtt tttattttat atgcacaatc ataatccttt tcatacttta ctttaattat
      181 atctataatt tcattatttg tatcattgat tttttctttt tttactaatc gttttgcaaa
      241 gaattttaaa tgagtcagaa gcctatcata atttatatca tcttctttaa attcaactct
      301 ataataatat cttataatat ttagtatgtc ttttacaatt ttagtaatta atagtgattc
      361 ctttgaactt cccttatagt ttgaatttac tatatgcatt gctataaaac cagcttcatc
      421 tactggaaat ttcacatcaa attctttatt tatataatct acagcccatt tcccaatctc
      481 atattctttt ttatgtattc tttttatctc atgtagtaaa tcattcttta tagttatgtt
      541 ttcatgatat cttttaattg caaatgcaat atggtcagta agtgctacat aaatatattc
      601 ataaagctta gtattcaagt tctcctctgc ataagcaatt atttcagtac taacctcaaa
      661 aaatttctca tcaacctggc ttgctagttt actaatttta tctaaaaagc tttcatcttg
      721 tattacaaaa gttctttcaa ttttactttt atctatttct tgacctttct ttttttgaaa
      781 cccaattcca catcctgtta atattaattc tttttttata ttttcatcta aaactaatac
      841 aacattatta ttataaattt gttgaataat cataatcaat atctctccca aaatacaatt
      901 tcccttattc ataaaaaaat acctaaatta agatacaatt cacctgtata ccttaaccta
      961 ggtattgcct gcttaccagt aacaatccag cttgttaatg cttatatatt gtattcaatt
     1021 tatggtttta ttctattata aataaatata tatgtcaagt aaattattga aaactatttc
     1081 ctatttttta gtattaattt taacatcaaa aatcaaattt tattccatta tatttatgat
     1141 tctatcatca tcatttcatc atagagaata ttttccaata actctttagc cacttctctt
     1201 gatatttgct tagtttcaac taattgttga acactgtctc tttgactttg tatcgccatt
     1261 actctgtagt attctactct tgcctcaaac ttatcatcat tttgaatctt tctagtaatc
     1321 caattcaaat aacgattata gaatctgatt gctattaagg cctcatgtct gttatcttca
     1381 tcagtatttt tatttataaa tgatatagca gttttagtag aaagtgcata tgcatggtga
     1441 aactctttga ttatttcttt attctcagat actatttttg taattttttc ttttacttct
     1501 ttctttactt ttctatttat aatccacatg cttattttta attttacaat tttaaatata
     1561 gatttttgaa cagattttct tgattttgta atataaaatc tatacaattt aaaggctcta
     1621 tcagaaattt taccatcttc taatagtttc aaaacagcta ataatccctg ttggtctgct
     1681 tctgcaatca tcatacttat aagtttatta tctggagtat ttagtattcc tttttctgag
     1741 tctaccaact gttcatttaa atcatgtata actggttgta cttcttgtgc tttttcatta
     1801 ctaagtttta aaatggcttc tttcaaaatt agtttatatg cctctttatc agataataaa
     1861 ggttctgact cttgtacgtt attttttaat aatcttggca gtaaaattgt tgccaatact
     1921 aaacttataa gaataacttc tgatgatata aataaaacag tatctctcat tggaaataca
     1981 tctttatcag ccaaataaaa aggtatagat aaggcagttg ctagtgtaaa tgtaccatga
     2041 acaccacaag tagcaactat tagtgagtat ttacatttag aaatactctc ttttataact
     2101 tcttcatcct taaaacctaa aaaacctaca aaaaaattat ttaaaggatt ctttttaggt
     2161 tttataaaag aattatgcca taaatatacc catataaatc ttataacaaa taaaataagt
     2221 acaattaaag tagaaataaa agtcaattcc aatgccatat tttcattttt actactaagt
     2281 cctgtatata tacttggtaa caaaaaacca agcagtgtaa agacaaatcc attcaataca
     2341 tactccaaaa cataccaagt attgtctgat attatcctta atttagtagt tgtattttgt
     2401 aaatgctctt tttcaattcc atgagctatc cctgccatta caacagctag gattccagat
     2461 actcctattt cttctgctac aaaatataca aaaagtggag tcattatttg tataactatt
     2521 accattggaa tttcttccaa attccacttg tgtaaactaa gtcttatttt tataattatg
     2581 tatcctaaaa tacttcctac tattattcct ccaaaagctg taatcataaa ttgaatccct
     2641 gcctcttcta ttgaaaaaac tcctgtaact gtagcaagta ctgcaacctt aaatgctact
     2701 actccagcag catcatttaa taaagattca ccttcaagta tactcattat atttttagga
     2761 aaatttaatc cttgtgtaat tgattttact gctactaaat cagttggaga tattactgct
     2821 gccaaagcaa atgaaacaga taatggcatc ccaggaataa gaaaatgtat aaagattcca
     2881 cctgcaaaca cggttattaa aactaaacca aatgcaagaa gcaaaattgg ttttctaagc
     2941 tctaatagct cttttctcga tacattttgt ccttcactga atagtaatgg agctatgata
     3001 catattacaa atatttctgg attaaatgaa aatgagaatt taaatggcaa aatagacaat
     3061 ataatcccta gtacaatttg atatattgca agaggaatat tactccactt tttgctaaat
     3121 acagttgaaa tcgttgtaca aaccaataat cccaacaatg ttactaaaat tgacataact
     3181 acctctcttt tcaataataa tattatagat taaacttata tatcaactaa cttaatatta
     3241 acagcatgaa atcccatctt aatcaaaaaa attttcagtt tattctatta ttcttattat
     3301 atacatcaaa tttaattttt gaaatatcta tactagatga catgcaacaa aatgatttct
     3361 tgatatttct tttaaaactg gaatctcttt tttgcatatt tccttacaat aagggcatct
     3421 tgtatgaaac ttacatccac ttggtggatt tgttggagat ggtatgtctc cttttaatat
     3481 aattctattt ttcttaattg tagggtcttg tattggtact gcgctcataa gtgcttttgt
     3541 atatggatgt tgagaatgta agtaaagttc tcctttagtt gcaatttcta ttatagaacc
     3601 tagatacata acacaaactc tatgtgcaat atgatttact acactcaaat catgagatat
     3661 aaataaatat gaaagtttat atttatcctg taaatccata agtaaattta taatttgaga
     3721 ttgaatagaa acatctagtg cagaaacagg ttcatcacaa actataaatt ctggatttaa
     3781 tgctaatgct cttgctatac ctattctctg tctttgacct ccagaaaact cacctggttt
     3841 tctatttaaa tggtacttag aaagaccaca actttctaca acatttaata tattatcttt
     3901 tatttcattc tttggcacta aattatgttc tataactgcc tcggatatta tttgttccac
     3961 atttaatctt ggatttaatg aactatatgg gtcttgaaat atcatttgca ttttgggtct
     4021 taactttcta agttcttcct tctctaactt atatatatct acaccattaa attcaacttt
     4081 accttcagtt ttttcaatta atctaagaat ggttcttcct gcagttgatt ttccacatcc
     4141 cgattctcca actatagcca aagtttctcc tttttttaaa tcaaaagaaa ttccatctac
     4201 tgcctttaca gatttaattt tcttatcaaa taatccagat ttaacttcaa agtacttttt
     4261 aagaccttct acttttaata aactctcttc ttttttcaat tatatctcct cctttttata
     4321 taaccaacat gcaatactat gaccactatt caattctatt aaaggaggta tctgagttct
     4381 acatttatca catacttttt cacatctatc actaaaataa catgaatctg gcattccaat
     4441 tggatttggt acttgtcctg gaatcgaata caatctttta tctttgtttt ttccaagagc
     4501 aggctttgat tttagaagtc caatagtata aggatgtttt ggatttttaa ataattcaac
     4561 aacctctgcc acttctataa ttttgccagc atacatcact aatactttgt cacacatttc
     4621 tgcaactact cccaaatcat gtgtaatcat cattatagat gtgtttaatt tttcttttat
     4681 atttttcatt atatctaaaa tctgagcttg aatagtaaca tctaaagctg ttgttggttc
     4741 atcagctata agtaattttg gattacatga taaagccata gctatcatta ttcgttgtct
     4801 cataccacct gataattcat gtggatatga attatatatt tcttcagctc ttggtatttc
     4861 taccagtttt atcatatcga ttaccttttt cttagcttct tcctttgata aatcttgatg
     4921 tatcataata ccttcaatga tttgacttcc aacagtaaac gctgggttta aagatgtcat
     4981 tggttcttga aaaatcattg atatctcatt ccctcttatt cccataagtt catcttcatt
     5041 catctttaaa atgtctttgc ctcgaaagat tatttctcca ccttctattt ttgatgttgg
     5101 aagtaacttc atcaaagaca tggctgttac agacttacca catcctgatt ctccaactat
     5161 tccaatagtt tcaccttcat ttatattaaa acttacttca tttacactct ttacaatccc
     5221 tttatctgta tgaaaataaa cttttaaatc tttaacctca attagtgctt tactcattat
     5281 tttctcctct cagcaaaatt acttaagata acatacacct tttgattgta ttttataaaa
     5341 atttaaatta caacattttc ttacatttta ttattttttt ctattaatta taataatata
     5401 atagttaatt tctatatata tattgattta ataatgatta ctattataca ataaaaaata
     5461 tgtgttaaat attattttat ggaggtttta taagaatatg aaatttaaaa aattggcatc
     5521 attaatctta gtttcatcat taatgcttac atttacagcc tgtgcttcca atgataaaga
     5581 caagtctagt ggaagcaaat ctggtggaac tgttgtaata cctatggcaa gtgacccaga
     5641 tataatcaat ggagcttttg caaacgttaa agaagactct cttgcatcaa atatagttta
     5701 tgctcctctt tatacttatg aaaaaggaaa tttagtaaat tatttagcag aaaaagtaga
     5761 ttttaaagat agtaaggaat taactataaa attaaaatcc aatttaaaat ggcatgatgg
     5821 aaagccaata actgctgaag atgtcttatt tacatttaat acagttttag acgaaaaaca
     5881 aaattcacca tcaagacaat acttattggt gggtgagaaa ccagttaaag tagaaaaaat
     5941 tgatgattta acagttaaga ttactttacc aactgcaagt gaatctttct tatatggtat
     6001 atctaaaatt tctccaatac caaaacatgt gtttgaagga gaatcaaata tagctaagtc
     6061 agaaaaaaat aataatccag ttggttctgg tgcattcaag tttaaggaat ggaaaaaagg
     6121 tgaaagcata gtatttgaaa agaatgcaga ttattttggt ggagaaccta aggcagattc
     6181 tatagcgcta aaaataattc ctaatgaagc atctcaagaa acagctttaa ataatggcga
     6241 aatttcactt atgaagacat ctgctgaagg atatgaaaag gcaaaatcaa actcaaactt
     6301 acaaacttat acttatagtg aagaaagatt aaactacatt gtatttaacc aaaatatatc
     6361 aaacatggct aacaaagaag taagacaagc cttatcatat gccttaaata gaaatgaaat
     6421 gatagaatca gcatatggaa aagaaggttc tgtacctgct aaatctatat tagttccaga
     6481 agcagacttc tatacagaag aaggtgttga aggatatgac caagatacaa ataaggctaa
     6541 agacttattg gataaaagtg gtgttaaaat tgataaatta aaaataggtt ataacacagg
     6601 tagatttggg cataaaaact atgccttagt agctcaacaa gagttgaaaa aaattggtat
     6661 agaagctgaa atagttcctt atgaaagtaa ggcattcttc aatatactat ttagtaatag
     6721 cactgaatgt gatatgtatg taaatggtta tgcttgggga ttagaaccaa acccatatag
     6781 aggtatgttt gaaactggtc aatattgcaa ccaaactaaa tattctaatg ctgaaataga
     6841 tgctttatgg gaaaaaggat ttactgagtt aaacaaggaa aaacgtgaag aaatatataa
     6901 gcaaatacaa caggatatat ctaaggatgc gcctatttac actatagatt atgaacaaaa
     6961 tttaatggca gctcaaaaaa atctaaaagg aataaaagat gctaaaccat ctcctgcaat
     7021 attatttgaa gattggtcta aactgtatgt agaataattg atgtaaaatt ttatattatt
     7081 aattagaaaa ggagatgtct aaaaataata ttgttttatt attttaaaac gagacatctc
     7141 ttttaattag gagaatttac ttatgttaaa attaatattg aatagagtaa aagttttaat
     7201 acctatgtta atcttaatat ctatattatc atttggactt ttagaattag ctcctggtga
     7261 cccagcagat gcatatataa atcctttaat gtcttcacag gatattgaaa atataagggt
     7321 taatatgggg cttgataaac cagtatatat aagatattta aactggttga aaaacacttt
     7381 aagtggaaac ttaggtattt cttatataaa ccatatgccc gttacagaac aaattatgga
     7441 aaaaatggga aatacattta ttcttatggg tacttctttg atattttcaa tattggtagc
     7501 tatacctctt ggaatatttt tagctgtaaa taaaaattca ataactagta aagtatcatc
     7561 tgtttttaat tatataggtg tttctatacc ttctttttgg ataggtatga tactcatttc
     7621 aattttttca gttaaactta acattttccc atcaggaggt atgcatacta ttggaaatga
     7681 tagtattgga gacttagtaa aacatttagt tttgcctgta ataactttag gtctatataa
     7741 tactgctatt tttacaaatt atgtagaagc tagtgtaaat gaacaattaa aaaaacaata
     7801 tgttattaca gcaagagcaa aaggattatc agaaaaagtc atactattta aacatgtctt
     7861 gaaaaattca ctgacatcat tggttacaat cctaggcatg agtatacaaa aattagtcac
     7921 tggtgcattt gttacagaag tagttttctc ttggccaggt atgggaagat taatgattga
     7981 ttcaattttt tcaagagatt atactgtaat tatggcaata actatgttat cagcactatt
     8041 tttaatttta ggtaatttag ttgctgatat actatactta ttaattgacc ctaaaataaa
     8101 atctagcaaa ggaggattct agttgtgttt acaagtatct taaaaaaatt aaaaaaagat
     8161 aaatcctttg cttttgcatg tattactctt agcataataa tcatattaat tatagcttct
     8221 tttatattaa atatagactc taatgcaatt gatactactt ctaaacttca agaacctagc
     8281 ttaaagcata tctttggtac agacgaactt ggtagagatt acttctctag agctctttat
     8341 ggtggaagga tttcacttag tgttggtatt ttatcaatgc ttatttctgt agtatttggt
     8401 actgctgtag gtgttattag tggatatatt ggaggtaagc tagaccaatt cttgatgaga
     8461 ttaatagata tgcttatgag tataccaagt ttcttagttt tgattgtatt aaatacttat
     8521 ctaacaccta aagtttccac tattatacta attattggat tcttaagttg gatggaagtt
     8581 tcaagaattg tacgtggcga aacattaaaa ataaaggaaa atgaatactg tctagctgct
     8641 aaagctactg gtatcaagac tagaaatata ctattaagac atatagtttt aaacttaaaa
     8701 gagactattg tagttgctgg ttcgttaaat attgctaatg ctatcttaac tgaatctgct
     8761 cttagttttt taggtcttgg aatccaattg cctcttgcat catggggtaa tatgcttcaa
     8821 gatgctcaaa gacatatttt tgacaagtta tatttagctg tatttccagg attacttata
     8881 tttttaacag tcatgagttt gactataata agcaaaaaat tttctagtaa tacttaacaa
     8941 attttatata gaaaataaaa acagtgatta tctaaagata tacactgttt ttatttttcg
     9001 tttaaaaaaa tacatatttt gtatattata actttaaata ctactctacc atgttatgta
     9061 aaacctatta ttgctctcaa acatatagat gaaaaactaa tagtttatta taataaaaat
     9121 tgatttattt atttgttatt ttaataacat ttttaagagt tctttttact aaagaaatct
     9181 ataataaatt taaatacatg ttggtctaca tggctataac gttctttttt aggtctacat
     9241 aatataactt tccatgtaat aggatgatta aaaggctttt ctataatttt gctattttca
     9301 aaaaaatcat gaactggtga tggtagaatt gttataaaat cagaatttgt ggttgatagc
     9361 aacaaaaaat cccatgaacc agacattata tatcttttta acttaacatt ttcatcagta
     9421 aatttttgca ttagcttatg atgtatcata aaagtaggat taaatattgc aagtaactgc
     9481 tcattcaaat cgttccaatc tatcttatcc ttcttagcaa gtggatgatt tatatttaaa
     9541 aatgctgtaa gttcatcttc ttgaagcaca tgttcagtta ctacactttt atcaatttta
     9601 tgtggatgta acaatactgc ataatctaat tcctgtaaga taagcattct atttaagtca
     9661 tatgcaccct tctctactat atcaaattct atatctggat tatttgcaac taactgtgca
     9721 actacatctg aaaatgctat tccaagtata agtggaggta ttcctattct aacttttcct
     9781 ttaaattgaa ctgaatcttc tcttaaatct tccatcatat ttttgtattc atttatgata
     9841 tgttctgcat ttacataaaa tctttctcct gctggtgtta acccatttaa cctgccttta
     9901 tacctttcaa ataaatatac atcttcactt tcttcaaaat tttttataat ttgacttaat
     9961 gctggttgag atatgtgaag tgtcttagat gctattgata aattaaaacc agatttcact
    10021 atttcaacaa aatatttcaa ctgttttata tccattttaa acccccttcc ccaaaagtat
    10081 aatttagatt atattttttc ttctaatgat attttatagg aaaatcaaca taatttcatt
    10141 aatattttaa caaaatattt ttctaaatct actataaatt tttatcctat aaatttttct
    10201 tatacactat ataaaaaatt agacttagac aaaacgcttt tttcgccgta atctataatt
    10261 agaaacagga aaacctaaag cattaggaat aaaagtaatt gaaatactta aaaacaagct
    10321 tgtattaaaa aagtacttta ttatagtgtt tcctaatttc tttattcaga aaatttttaa
    10381 ttatcattca agaaaggtaa ctattagata tgaaagatgt agtaatcgta tcagcagtca
    10441 gaacacctat aggaagtttt ggaggtgtat ttaaaaatac ttcagctgtt caacttggaa
    10501 ctatcactgt taaagaagcg atatcacgtg taggacttaa tttatctgaa atagatgaag
    10561 taataattgg aaatgttctt caaacagggc ttggacagaa tgttgctaga caaattgcta
    10621 taaatgcagg tataccaaat tctgttccaa gctacacagt aaataaatta tgtggttcag
    10681 ggcttaaaag tgtccagcta gctgcccaat caatcacatc tggtgaaaat gatgtggtaa
    10741 ttgcaggtgg tactgaaaat atgagccaag ctccttatat cgttccaact gctcgctttg
    10801 gtagtaaaat gggtgacata accatggtag atagtatgct aacagatggc cttattgatg
    10861 ctttcaatca gtatcatatg ggaataactg cagaaaatat tgcaactaaa tttgaattta
    10921 ctagagaaat gcaagataaa ttggcattag aaagtcaaag taaggctgag aatgctataa
    10981 aaaacaaccg ctttaaagaa gaaatcgttc cagtagatgt tttaatacgt cgaggaaaaa
    11041 tagaaactat tgataaagat gaatatccta aattaggtat gacatttgaa ggtttagcta
    11101 aacttaaacc tgcattcaaa aaagatggta ctgtaacagc tggaaatgcc tcaggtatta
    11161 atgatggtgc ggctatgtta atactaatga gccaacaaaa agcagacgaa ctaggtatta
    11221 gaccacttgc taaaataaaa tcctatgctt cagctggtgt tgaacctgaa gtcatgggta
    11281 ctggtccaat ccctgcaact agaaaagcac tgaaaaaagc tggacttagc atcaatgata
    11341 tagacttaat agaagcaaat gaagcgtttg ctgcccaagc attagctgtt aaaaatgaac
    11401 ttcaaataga ttcatcaaag ttaaatgtta atggtggtgc aatagcatta ggacatccaa
    11461 taggagcaag tggagctaga atacttgtta ccttaattta tgaaatgcaa aaacgtaaag
    11521 ttgaaactgg acttgcaaca ctttgtattg gtggtggtca aggaatatca atggtggttt
    11581 cacgatagga ggaaatatga ataaaatagt tagcattgat gaagctctat ctcatgtaaa
    11641 agatggagat acaataatgg ttggtggatt catggccaat ggttcaccag aaaatttaat
    11701 tgattattta tgtagtaaaa atataaaaga tttgacacta atttgcaatg atactggctt
    11761 tattgacaaa ggagttggca aaatggttgt aaataagcaa tttaaaaaga taattgcttc
    11821 tcatgtagga ttaaataaag aaactggaag acaaatgaat aacaatgaaa cagatgttga
    11881 gttagttcct cagggaactc ttgctgaaca aatacgtgca gctggttatg gtctaggagg
    11941 tatactcaca ccgactggta ttggtactct tgttgaggag ggaaagcaaa aaattgttgt
    12001 cgatagcaaa gaataccttc ttgaaaaacc aatttatgca gatgttgctc ttctatttgc
    12061 atcaaaggtc gataaagcag gtaatctagt ttataaaggc tctatgaaca attttaataa
    12121 tttaatggca tctgctgcca aaattacaat agttgaagca gatgaaatcg ttgaaattgg
    12181 tgatattgac ccaaatgaag ttaatacacc aggtattttt gtgaattaca tcattgaagg
    12241 aggtaattta taatggataa attagaaatg caagaatata ttgcaaatag agtatctaaa
    12301 gaattaaaag acggagccgt tgtaaacctt ggaataggtc taccaaccaa agtagcaaat
    12361 tatattccgg ataatgtaaa cgttatctta caatctgaaa atggcttttt aggtttaggt
    12421 tcagccgaag atggaaattc aagtgatgaa actattgtaa atgcaggtgg tcaaccagtt
    12481 actatacttc ctggaggatg tttctttgat agtgctactt cttttggaat aatcagagga
    12541 ggtcatgttg atataactgt tcttggcgca cttcaggttg acaaatatgg taatatagca
    12601 aactatatga ttccaggtaa gatggttcca ggtatgggtg gagctatgga cctagtaact
    12661 ggagctaaaa aagttattgt agcaatggaa catacttcaa aaggttctgc aaaaatatta
    12721 aataattgta ctcttccact tactgcaaca aatgctgtag atttgatagt tactgaaatg
    12781 ggagttatgg aagtaacatc agatggaatt ttacttaaag aaataaatcc agctttcaca
    12841 ttagatgatg ttatttctgc tactgaggct cctttaatat tgtcagattc attaaatgga
    12901 aaaatatctg ttgtttaaaa taattaaaac tagaaaataa aaatctctta gacgtattac
    12961 atataaattt gtattgctta aaatattaat ataaaaataa aataaaattg gaggaaatga
    13021 aaatggttaa agataaagtt gtattcgtaa caggagctgc aagtggaata ggtaaacaaa
    13081 tagctgaatc gttcttaaaa aatggtgcta aagtaatgtt ctctgatata aatcaagaag
    13141 ctttaaataa taccataaca gagttacaaa aaaaaggtta tgattgcatg agtgtaaagt
    13201 gtgatgtaac taaggaagaa gaaattaacc acgctataga caaaactgtt gaaaagtatg
    13261 gcagattaga tatattaata aacaatgctg gacttcaaca tgtttctatg atagaagatt
    13321 tcccaactga taaatttgaa tttatgataa aaataatgct gacagcacct tttatagcta
    13381 ctaaaaaagc atttcctata atgaaaaaac aaggctttgg tcgtataata aatatggcct
    13441 caatcaatgg tgtaatagga tttgctggaa aggctgctta taactcagca aaacatggac
    13501 ttattggact aacaaaagtt gctgctcttg aagctgccga ttctggtata acagttaatg
    13561 ctttatgccc tggatatgta gatactccac ttgtcagagg tcagttcaat gacctttcaa
    13621 aaactcgtaa tataccattg gaaaatgtct tagaggaagt tttataccca cttgtaccac
    13681 aaaaacgcct attagatgtt caagaaattg ccgactatgc tatgttctta gcaagtgata
    13741 atgctaaggg tattacagga caagcttgct tactagatgg aggatatact gcacaatagt
    13801 tattatattt agggcttgaa gaaagtattc ttcaggtcct tctactaagg agggatattt
    13861 atgagtatta tcgcaccaat tggtataatt attggtatca tagctattat atatttttca
    13921 gttaaagaaa ttcatataac tatagctgct ccaatagcaa ctttaatagt tgttatttta
    13981 aataaaatgg atgttgtaac atcaatgcta ggtgctgaca aaaacaatta tatgggtgca
    14041 cttggaaact atataatgag ttattttgca atattcttat taggctcaat acttgctaaa
    14101 tttatggaag aaagtggagc tactgtatca atagctgatt ttattttaaa taaatttggc
    14161 tctaatcatc cttatagagt tttagttgct atatttatag taagttttat attaacgtat
    14221 ggtggtataa gcttatttgt agttatgttt gcggtcattc cacttgcaag aactctattt
    14281 aaaaaacttg atatttcttg gaaccttatt cagataccac tttggcttgg tattgcaaca
    14341 attactatga ctataattcc tggaacacca gccattcaaa atgttatacc tattcaatat
    14401 ctaaatacgt ctcttactgc agctactata ccaagtattg taggtagtat aggttgtgct
    14461 acgtttggac ttgtctatat gaaatatgct ctaaacaaaa gtattagaaa aggtgaaact
    14521 tatgcaactt atacttcaga attagaagaa gaatctttag atagagaatt accaggattt
    14581 tttgcaagta tttttcctct attagcactc gtaattatag ctataactgg aagtactttt
    14641 ggttcagaat ttatgaaaaa aaatgtaatt tacatagctt taatagttgc aattctttta
    14701 tctgcttttt tattccataa atttctatca tctaagatac agacattgag cataggtgct
    14761 agtggttcta taggtcctat atttgctaca tcttctgcag ttgcatttgg agcagtaata
    14821 atggctgcac ctggttttga tgtatttagc aaacttataa tgtcaatacc aggtagtcca
    14881 cttataagtc ttactgtttt gacttcaaca atgtcagcaa taacagggtc ctcttctggt
    14941 gcattaggaa tagtaatgcc aaactttgca gattattatt taagtactgg tctaaatcca
    15001 gaattaattc accgtgttgc tacaatagct tcaaatatac taactatagt tccacaaagt
    15061 ggtgtttttc ttacattctt aagcttatct aagttaacac ataaaactgg atttaaagaa
    15121 actttcattg tagttgcagt tggttcacta atagcagaaa ttattgtaat tacattagga
    15181 ttactaatgt attaatataa aacaaattaa aattcataat atgaaaggtg gtgtctcaaa
    15241 gtgaactttt tacttaatga gacactcttt ttatatgaaa taacatatgc ctatcattta
    15301 aacaataaaa aaggccttat ctctattttg agacagaccc tttttatttc ttaaagattt
    15361 aaataattat aatttgttgc taatacgaaa gcatctactg aataaattta accaaataaa
    15421 aaaaaattta tcataactat aaatttacgt tatggaatta ttataaaaat attattttct
    15481 ctaactaagt aattccatac tatacaggct tttaatacaa attgtcaaac acactgaaat
    15541 atacctattt attccatata tatctttttt acatatcttt acagtaaaaa aagtcttaat
    15601 cttatgttag tatagattca cgggtaaccc cctacactaa aggttacaaa tcagtaacat
    15661 ttttagaatt tttagattaa gaggtgttta atgatgagaa gaaatatacc aacaaaatta
    15721 aaaactatat tagctttagg agtgttaact ttagcaacag cttcaagtgc tgttactgca
    15781 agtgcttcat cacttgaaaa taatgtaaat ataagtaaca aaagtttagt atatagtaat
    15841 actaaataca agttttctgc aaaaccaaat tgttctgtaa aaccagaact taagcctgat
    15901 aaagataact cttgtaagga taagaatcat aatgacaaga attgcaacaa cactaataaa
    15961 cctgaggaca acaataattc agactctact aataagcctg gtaacaataa acctgagtct
    16021 aataaacctg aagacaacaa taattcaggc tctactaata aacctgataa taataaacct
    16081 gagtctaata aacctgaaga caacaataat tcaggctcta ctgataaacc tgaagacaac
    16141 aataattcag actctactaa caaacctgat gataataaca actcaggctc tacaagtgaa
    16201 agtttttcag cttaccaaaa agaagttgtt gacttagtaa atatagaaag agctaaagct
    16261 ggattaaatc ctcttactct tgattcttct atatcaaatg ttgctacaaa aaaatctcaa
    16321 gatatgattg ataacaacta tttttcacac aattctccaa cttatggttc tccatttgac
    16381 atgttgaaaa aatttggaat aagctataag acagcaggtg aaaatatagc tatgggtcaa
    16441 aaaactccta aagaagttgt aaatgcatgg atgaattctg aaggtcacag aaaaaatata
    16501 atgaatccaa atttcagtaa aataggtgtt ggagtagcac aaaaaagtgg tggttcaatt
    16561 tattggacac aaatctttgt aggataaaat aatctttttc catacatatc aaaagattat
    16621 ctctaagtga taaaaaacac aaatgagata ctcttttgta tctattttaa aattatcaaa
    16681 tccaaatata catactataa atattatttg ttctcttaat tttagtgtgt agagcaatat
    16741 ataaaaagta ttggcaagat gaaaatggaa aacttaaaaa gatatttaat aggtaaaaac
    16801 aggatcttaa tatcttcata aaaaaaggct gtctcaaaat agagataagg cctttttcat
    16861 tgttgaaatg attaaaatat atttgatttc atacaaaaag agtgcctcat gaactaaaaa
    16921 gctcactttg agacactctc ttttatcaat tctatataag cattataata ctttaatatt
    16981 attcagctaa ttttttgtat ccttcatatc tttctttagc atcagcttca gctttagcaa
    17041 ataatccatc agcttcctct gggaattgtt tagctatagc agaatatctt acttgtgcaa
    17101 gtatgaaatc tttgaagctt gcagttggtt cttttgaatc taaagtgaat gggtttttac
    17161 cttcagcttt tacttctgga ttgaatctgt ataaatgcca gtatccgcaa gcaacagctt
    17221 gtgcttcgtt agctacagtt cttcccatac cttcttttaa tccatggttt atacatggag
    17281 cataagctat tattaaagat ggcccatcat gtttttcagc ttctataacg gctttgataa
    17341 attggttttt atcagcaccc atagcaactt gagctacata tacgtttcca tatgtcatag
    17401 ccatcattcc taaatctttc tttctagatt ttttacctga agcagcaaac ttagccatag
    17461 cagcagctgg agtagcttta gaagcttgac ctcctgtatt tgaatatact tctgtatcaa
    17521 atacaagtac gtttacatct tcaccagatg caagaacatg gtctaatcca ccgtaaccga
    17581 tatcatatgc ccaaccgtct ccacctagta tccattgaga tctcttaact agataatctt
    17641 ttctatcttt tatagctttt actaactctt tagctttttc atctgtacaa tcacaagctg
    17701 gtttttctaa taattcaaca accttagcac ttgctacttt agaagcttct ccatcatttc
    17761 tagaatctaa ccactctgta aataccgctt tagcatcttc acagatatcc atagaaacta
    17821 actctgtcat agcatctact attttatttc ttatttgctt aacagctgtg tacataccaa
    17881 atccatattc agcattatcc tcgaataaag agtttgccca agatggacct ttaccttcat
    17941 ggttacaagt ataaggaatt gatggtgcag aagctcccca tatagaagaa catccagtag
    18001 cattagctat catcattcta tctccatata attgtgtaac tactttaatg tatgcagttt
    18061 ctccacatcc agcacatgct cctgaaaact ccattaatgg ttgtctaaat tgacttcctt
    18121 taacagtgtt tgcaggcata acatcacctt ttggagctac ctcatcagga tttactgcaa
    18181 atgcccaatt atctaactct gtatcaagtt gagtatctat tggcttcata actaaagctt
    18241 tttctttagc tggacatatg tcagcacagt ttccacatcc tgtacagtca agtggactaa
    18301 gttgcattct atattttaat ccttctaatc ctttacctaa tgctttttta gcttcaaatc
    18361 cttctggagc attagctaat tcttcttcag taactaatac tggtcttata catgaatgag
    18421 gacatataaa agaacattgg ttacattgaa tacagttatc tattatccat tcaggaacat
    18481 caacagctat accacgtttt tcataagctg cagtaccaca agggaatgtt ccatcttcta
    18541 taccattgaa tgtacttact ggtaagttgt ttccttcttg tgcagtcatt ggtcttagta
    18601 tattttttat aaattctggt tctttaactt ctcctttatc tgcatcaaca gcattttccc
    18661 aagaagctgg aacttcaact ttaactaaag catttattcc tgcatcaaca gcagcatagt
    18721 tcatgttaac tattttttca ccttttttac cataagcttt aactatagaa tcttttaagt
    18781 atttaacagc atcttcttct ggtattattt cagctaattt aaagaatgca gattgcatta
    18841 tcatgtttat tctgtttcca agacctattt cttgtcctag tttaacagcg ttaactgtat
    18901 agaattttat atcattttta gctatatatt gtttcatatg agctggtaaa tgttcttcaa
    18961 gtcctgcttg gtcccatata gtgttaagta caaatgtacc acctttttta agacctttta
    19021 ataaatcata ttgataaaca tatgattgtt tatgacaggc aatataatca gcttcatcta
    19081 ttaaataagt agctcttatt ggagtatcac caaatcttaa gtgagacata gttataccac
    19141 cagatttttt agagtcataa tcaaagtaag cttgagcata tttttcagta tggtcaccga
    19201 ttattttgat agcttgttta ttagctccaa cagtaccgtc agaacctaat ccccagaact
    19261 tacatcttat agttccttta gaagctattt ttattggctc agatggagtt aaagaagtat
    19321 tagttacatc atcaactata cctactgtaa atccattttt aggttgttct gaaactaagt
    19381 tatcaaaaac agtttttata tctgaaggag tagtatcttt tgaacctaat ccgtatcttc
    19441 cacctattat cataggagca ttttcttttc catagaatac atcacgtaca tctaagtata
    19501 atggttcacc agttgaacct ggctctttag ttctatcaag tacacatatt ctttcaacag
    19561 tagatggcat agcatctaag aaatgtttca ttgagaatgg tctatataaa tgaactttaa
    19621 ctaaaccata tttttctccc ttagcattta agtaatctat agtttcttct attgtttctg
    19681 ttactgaacc catagctatt aatacgtatt tagcatcttc tgctccatag taatcaaata
    19741 aactatgttt tctacctgtt aaattactca ttttttccat gtatttttca actataccta
    19801 ctatattttg atagtattta ttagaagctt ctctagtttg gaaatatata tctgggtttt
    19861 gagcagtacc acgagcaact ggatgatttg gagataaagc attatctcta aatgcttgaa
    19921 ctgcttcaaa gtttaataaa cttgcataat cttcattttc tagtaattca actttttgga
    19981 tttcatgtga agttctgaat ccatcaaaga aatgtataaa tggtaatcta ccttctattg
    20041 cagctaaatg tgcaacaggt gctatatcag ctacttcttg aactgaacca gaagctaata
    20101 atacacatcc agtttgtcta gcagccataa cgtcttggtg atccccaaat attgataaag
    20161 cttgagatgc taaagcacga gcacttacat ggaatactcc tggtaacaac tctcctgcaa
    20221 ctttatacat gttaggtatc attaataata aaccttgaga tgctgtaaaa gtagaagtta
    20281 aagctcctcc ttgtaaagaa ccgtggaatg ttcctgatgc acctgcttct gactgcattt
    20341 caacaacatt aactgtttgt ccaaatatat tttttcttcc ttgtgatgcc cactcatcaa
    20401 ctacttcagc catagttgaa gatggtgtga ttggatatat agctgctaca tctgtaaacg
    20461 cataggcaac atgagctgca gctgtatttc catcaagtgt tttcataaac ttagccataa
    20521 tatgtatcct ccttgatagc tacaattgta cttgtttata acaagtctat ttttttatat
    20581 ttacatataa tttacacaac aaaactataa acacacatat agtcgcgcaa atactctatc
    20641 ttctaatata atactacaga ttagataaaa ttcctttgaa aattgtaaat taattacata
    20701 tttaagtaga ttttttttaa aattaaatat gttaagtttt tttcata
//
LOCUS       DAMJVI010000066        20710 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.068, whole genome shotgun sequence.
ACCESSION   DAMJVI010000066 DAMJVI010000000
VERSION     DAMJVI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.068"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            65..1942
                     /locus_tag="PC545_002453"
     CDS             65..1942
                     /locus_tag="PC545_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2457220.1"
                     /translation="MVLGSKSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTG
                     AYEGTINYNQDPEYLNVWELQGITINSKNNPKTLNRQDLEKLGLNLKDYNVTQECIIE
                     DITSRKDVNKYLRKTSSPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGI
                     ISTPLATTYNAPILLVEKNNVPNSVKSELKRLNPKDIIIIGDENAISKTTANQIKSTV
                     NASQTRLNGSNRYETSLLIAKEIDKNHDVEKIYITNANGGEVDALTIAAKAGQDKQPI
                     ILTDKDSITDNTYKWLKSEDLQNAYFIGGPQMISTNVINKVNGITKDSVTNNRVYGAD
                     RHETNANVIKKFYTDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYH
                     KDNLEAKSANKVYKIGGGLTSKVMSSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGS
                     APGNSSGGMIEKDYNLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLKPD
                     LFTSIHYNGSTNKQGHGVEVFYKLKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRV
                     LPSDSTKDYLYVLRSNDMPAVLVECAFLDNENDMSLINSSAKVKEMGTQIGKGIEDSL
                     K"
     gene            2184..4097
                     /locus_tag="PC545_002454"
     CDS             2184..4097
                     /locus_tag="PC545_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457221.1"
                     /translation="MEYLFEKSSLKEYGNEALDKYRELLDKTGNNSRCILLLVPNNNT
                     KIRYERALRLNYSEELKVTTYISFVKKELVKYWPLIIEKCDGISTKVVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIANSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNALSIYLYNQYLLNDDFYLKNLFTEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDIDYINEKIISKIK
                     PKEENIKKKEIRIEDLYLLPANIELNQSSQLYNEMLDLISEKVISLIEEDVSPRDIAI
                     ISPINNSILEHQIRDSLIEKNIDVFSTKKDKKAVDYPYGNALVVATCIFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNIDEHSQYRDILQYIEEKKKSDIKIHE
                     FLTQFYIDKMLNLKEGKKNVGLCKKIIAESESFSESLSLLGMKKEKIFVEALKSTIND
                     YYSVVDIEELKSKDKIVITTPYSYISSNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAFKSEYAINGYIQESILYSI
                     LLKISHKGDKNYD"
     gene            4090..6309
                     /locus_tag="PC545_002455"
     CDS             4090..6309
                     /locus_tag="PC545_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDF2457222.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKSGKILILTYMNSAVNNFKGRIRKILEENKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKSIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGHKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENKNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVKYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINVKWYESWKIEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLTFEELGPNSLNKRKVINNIAYIIDFMLHCDD
                     VEKLIVVLNKVFIDTDNEEEIKYFLEELKNYSTEEIIYNFEFSKLDNDLIEFKEESDI
                     YLSFKRGIDIIKQILEYPIIRLDLLVLFIGKKLSLEKEDKAVLDYISFYIKYLVSENI
                     NMDLEDVYNLLFDVKNRVFNHIIDVVYEINGYEPEPGSITLCNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLSRLNQHIIEKRSN"
     gene            6418..7248
                     /locus_tag="PC545_002456"
     CDS             6418..7248
                     /locus_tag="PC545_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDF2457223.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDDKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMTLNGDIIQAKYDLVVIGNNNIEIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYDEEKHEANRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            7238..10705
                     /gene="addB"
                     /locus_tag="PC545_002457"
     CDS             7238..10705
                     /gene="addB"
                     /locus_tag="PC545_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HDF2457224.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRVLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQSGFVSSITDMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDINYIGYSKSDMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSNQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     DKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINLLENYVLANGIKGKKWFDEKWDYRITQSLSGQ
                     ESEFELELKEKINEIKNRVLEPIVILQEKLRGKNRVKEICRYIYEFLLDINMPETIES
                     LIINFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSKTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDKDILKKITVKSPTFNELINVIKNYDSDGYNTEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHKIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKAKKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLTWRDIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGNNG
                     KYPPIKMVLENGQEVSLIGQIDRVDEFEEGENKYIRIIDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDEDININPAAILYCRINNPIAKFNEDKDDEEIQEAILKELRMKG
                     LVVKDSHIVKEMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVRH
                     AIKDLCEEMLSGEIRIAPYKHKDGTSCDFCDYSAICQFDSTIKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            10705..14532
                     /gene="addA"
                     /locus_tag="PC545_002458"
     CDS             10705..14532
                     /gene="addA"
                     /locus_tag="PC545_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDF2457225.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRINLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSVEYKKIADISQACNKSWDEAYKK
                     MASMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISSVVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNKFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFNVDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRINNKENEIEEEEK
                     LDNIQLEARMVGNIIKDLMKVNEDGKIQKVYDKGIDGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYDGFTDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGIESLDTISQYEILKGKNFLDWIMPCVLRHRDLSN
                     LLEEVGLDAVFNVEHNSKWYGKLWNKSDILVEKKSDEEKESIEEILEKIDVDNPDSDY
                     YSEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEEREKISGTERGTIVHLVMEVLDLKNVSSVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFASNIGKRMLNAEIINREKSIYAQVNMKDIYIYEKLINNDDKKLYDNESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            14578..15789
                     /locus_tag="PC545_002459"
     CDS             14578..15789
                     /locus_tag="PC545_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDF2457226.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITYATQGCTKLNINGENIVVATLPYPSEKRLNEVFSSE
                     DEFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDREIWSYFEINTDEIISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            15776..19303
                     /locus_tag="PC545_002460"
     CDS             15776..19303
                     /locus_tag="PC545_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2457227.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNIQLQNLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQKSYEESKTVYEEQLKLEKNELRKNELDLKNSEIKEKI
                     ALVENDSNAKKVDPYISSVQNLEKKIDEDSFAVSNLEKKLAILNQELEVTKNRYEKIN
                     KIKNEEIPKLSEEKIRLQQAIKLEEELVLLDRELKELKESGINLNKTKVELEKVKQVS
                     ESRKDAVTKSIKEVEGKIDKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKLNKLEEI
                     LKETENINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KANNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEELK
                     YLNLASELRRELKENMPCPVCGSKHHENHDITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKVSGYLSIEKMKTKELEDVKGKLGGIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYLGLKTITKVSNLLSKVKEIRENEKELELLSTGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYVEKKLSRDNKYKEVISITKGDLAKNLLHNVEENICKILEQEESSKK
                     KLEEQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     TDAVKKLIEEILEHEEEQKLLSFKIKSSKEKLNGRSIKKEYFEQLKDEIYNLKVEIGN
                     ISKDIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            19434..20687
                     /locus_tag="PC545_002461"
     CDS             19434..20687
                     /locus_tag="PC545_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDF2457228.1"
                     /translation="MLESPNNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
BASE COUNT         8989 a         1957 c         3677 g         6087 t
ORIGIN      
        1 tgctactgaa tctaatgcta tggaaaataa cgctgattta aatataaact tagagaaaaa
       61 aagtatcgtt ttaggtagca aatcaaaagt tagtgtcaaa tttaaagaaa aaccagatgc
      121 agatagcatt acattaaagt ataaatgcta tgacatgcca ttggatacaa ctctaaatta
      181 caatcaatca actggggcat atgaaggaac tatcaattat aaccaagacc cagaatatct
      241 aaatgtttgg gaactacaag ggataacaat aaacagcaaa aataatccta aaactttaaa
      301 cagacaagac ctagaaaagc tgggattaaa tttaaaagac tataatgtaa cacaggaatg
      361 tataattgaa gacataactt ctagaaaaga tgtaaataaa tatttgagaa aaacttcttc
      421 acctattaca gaacttacag gaagtgatag atatgaaaca gcagttaaaa taagtaaaga
      481 gggttggaaa aatggttcag ataaagtagt tataataaat ggggatgtaa gtatagatgg
      541 cattatatca actccactgg caaccacata taatgctcca atacttttgg ttgaaaaaaa
      601 caatgtgcct aatagtgtaa aatcagaatt aaagcgccta aaccctaaag atataattat
      661 aattggagat gagaatgcta tttctaaaac tactgctaat caaattaaat caactgtaaa
      721 tgctagtcaa acacgtttaa atggttctaa tagatatgag acatctttat tgatagcaaa
      781 ggaaatagat aaaaatcatg atgtggaaaa aatatacata acaaatgcta atggcggaga
      841 agtggatgca cttactatag cagcaaaagc aggtcaagac aagcaaccaa ttatattaac
      901 tgataaagat agtattacag acaatacata taaatggtta aagagtgagg atttacaaaa
      961 tgcttatttt ataggtggtc ctcaaatgat atcaacaaat gttataaata aggtaaatgg
     1021 aataactaag gatagtgtta ctaataatag agtatacgga gcagatagac acgaaacaaa
     1081 tgcaaacgta ataaaaaaat tctatacaga tgatgagtta gaggctgttt tagtagctaa
     1141 atcagatgta cttgttgatg ctttagcagc aggtccattg gctgcgaact taaaatctcc
     1201 aatacttata acaccaaaga cgtatgtatc tgcataccat aaagataatt tagaagctaa
     1261 atcagctaat aaagtataca aaataggagg aggattgact tctaaggtaa tgagctctat
     1321 agcatcatca ttatctaaac acaatacgac tccaacagaa ccaggaaata gtgggggcaa
     1381 gacagttatg attgacccag ggcatggtgg ttcagcacct ggaaattcat ctggaggaat
     1441 gattgaaaaa gattacaatt taaatacttc acttgcaaca actgaatatt tacgttcaaa
     1501 gggattcaat gtaataatga caagagacac agataagact ttatctcttg gaaatagaac
     1561 tgctctatct aattcattga aaccagattt atttacaagt atacattata atggctcaac
     1621 taataaacaa ggtcatggtg tagaagtatt ttataagctt aaagataaaa atggagggac
     1681 tactaaaact gtagctacca atatattaaa tagaatttta gagaaattta aacttacaaa
     1741 tagaggtata aaaacaagag tacttcctag tgattctaca aaagattatt tatacgtttt
     1801 aagaagtaat gatatgccag ctgtacttgt agaatgtgca tttttggata atgaaaatga
     1861 tatgagttta ataaactcat ctgcaaaagt aaaagaaatg ggtacacaaa taggtaaagg
     1921 aatagaagat tcattaaaat aaatggaaat aaaaatattt aaaaacttaa tgtaaattta
     1981 tagttgtaaa tttatactaa agtcaaaaaa tgaaatataa gtgtatcaat agtaagactg
     2041 tatgtacatt tgcacatgtg aatgcaagta tatatacagt ctttttttgt agttaaatgc
     2101 aataatttga ttgtgttttg taaaatacac aatatgatat aattagataa tatacactta
     2161 atttaattga ggaaggtgat tatatggaat atttatttga aaaaagtagt ttaaaagaat
     2221 atggaaatga agctttagat aaatatagag aattattaga taaaactggt aataattcaa
     2281 ggtgtattct tttgttagta ccaaacaata acacaaagat acgatatgaa agggctctaa
     2341 gattaaatta tagtgaagaa ctgaaagtaa caacatatat aagttttgtg aagaaagaat
     2401 tagttaaata ctggccacta ataatagaaa aatgtgatgg aataagtaca aaggttgtat
     2461 ctccaacatt tatatcaaat agtttaagtg aatatttaat agtgcaaaaa gttaaggaaa
     2521 aaaggataca agaaggatat tttgaagata ttacaggaac caataaatcc atagcaaata
     2581 gtataatgac taacataaat aagtctgcat ttaatttaat tgattttgat tttattggag
     2641 aaaaaatata ttcatcaaag aaaaataaag atagtatata tagattttca tacactcaaa
     2701 tggatgaaat tatatcatat tatataaatt ccttactatc aaattcaatt ttagataatg
     2761 cattaagcat atatttatac aatcaatacc tacttaatga tgatttttat ttaaaaaact
     2821 tatttacaga agtgagatat ataattgtgg atagccttga aagctcaagt aatgcagaag
     2881 ttgattttat aacagaagca ttggataata cattacaaag ttatatatat tttgattata
     2941 gtagggatta ttctgtattt aataatgtgg atatagacta tataaatgag aaaataatat
     3001 caaaaattaa gcctaaagaa gaaaatatta aaaagaagga gattagaata gaagatttat
     3061 acttattacc tgcaaatata gaattaaatc aatcaagtca attatataat gaaatgttag
     3121 atttaataag tgaaaaagtt atatctctta tagaagaaga tgtatctcca agagatatag
     3181 caattatatc tcctataaac aatagtatat tggagcatca aattagagat tctttgatag
     3241 aaaaaaatat agatgttttt agtactaaga aggataaaaa agctgtggat tatccatatg
     3301 ggaatgcatt ggtagtggct acatgtatat tctatggata tttagatttt ataaaagatg
     3361 atgaatactt aagcttttta gaaactcttt tagaaataaa taggataaaa gcatttaaga
     3421 tttttaatga aacaagacat ttaagtattg ataatataga tgaacatagt cagtatcgtg
     3481 atattttaca gtatattgaa gaaaaaaaga agagtgatat aaaaatacat gaatttttaa
     3541 ctcaatttta catagataaa atgttaaatt taaaagaagg taaaaagaat gttgggttat
     3601 gtaaaaagat aatagctgaa agtgagtctt ttagtgaaag tttaagtcta cttgggatga
     3661 aaaaagaaaa gatatttgta gaagctttaa aaagtacaat aaacgattat tactctgttg
     3721 tcgatataga agagttgaaa tccaaagaca aaatagtaat aactactcct tattcatata
     3781 tatcatcaaa tatagataga agtatacaaa tatgggtaga tataggaagt aatgcttgga
     3841 atatgaaaat agaaaaagat ataagtaatt taattgtact tagaaaatct tttgaagaaa
     3901 aaaagattta tacaaatgaa atggaagaat attataaaaa atattatcta tacaatacag
     3961 tatataattt gttattaaat gcaaagaaaa tctatgcatt taaaagtgaa tatgcgatta
     4021 atggttatat ccaagagagt atattatata gtatactttt aaaaatctca cataaaggag
     4081 ataaaaatta tgattaatat aaattataga gaagaccaga taccaataat aaattacgat
     4141 aatggtacta tggcagtacc agcagtacca ggtgctggaa aaacatttat aataacaaat
     4201 ttagtagcta aactattact tgaacagaaa cataagagtg gtaaaatatt aattcttaca
     4261 tatatgaata gtgctgtaaa taattttaaa ggtagaataa gaaagattct tgaagagaat
     4321 aaaatagatg atactaattc gtatgaagta atgactatac acagtttggc tgtaaaaata
     4381 ataaaggaaa agccagaagt tgtaatgtta agtgaggatt ttaacatagc tgacgatttg
     4441 caaaaaagta ttatattaaa tgaatgtata agtagattta gatttgaagg tggagaaaga
     4501 gcttttagat ggtttttaaa ggagcaaaaa gatgaacgct ggaaagaaat aactcttgaa
     4561 gcctgggaaa gaggtttttt tgaatttgca ggaaatgcaa taagtgaact taagtataag
     4621 ggaatttctc cagataaact tgaagaaata ttgtcaagag gacacaaagg aatgcttaaa
     4681 attattttac caatatataa attgtatgat agaaaattaa agcaaaatgg attgctagat
     4741 tatgatgata tattaatttt agcagaaaaa actttaagct tagacgaagg gcttagaaag
     4801 aagtttcaaa ctagatacaa atatatattt gaagatgagt gtcaggactc aaatgaaatt
     4861 caaggtaata taataaaaat tatatctagc gaaaataaaa acctagttag agttggagat
     4921 ataaatcaga gtataacagg aacattttca tcatcagacc ctaaattttt taaagaattt
     4981 ataaatagtg cagatttctg ttataggatg gatatgtcaa atcgaagttc aaaagatata
     5041 cttgatttag caaacactct tgtcaagtat gttacaaagg aatttagaca agaagaatgt
     5101 agacatgctc ttgaagatat gcaaataaaa actgtgccaa atggaatggg atataaagaa
     5161 aaccctaatc cagaacatta taatataaat gtaaagtggt atgagagttg gaaaattgaa
     5221 atagagcaaa ctgctagata cgttaagggt ataaaaaata aatatccaga taaaagtata
     5281 ggtatattgg ttccatttaa tgaacaagtg acacaagttg ctaaagaact tagagagaat
     5341 aatctaacct ttgaagaatt aggaccaaat tctttgaata aaaggaaggt tataaacaac
     5401 atagcatata ttattgattt tatgttacat tgtgatgatg tagaaaagtt aatagttgta
     5461 cttaataaag tttttataga tactgataat gaagaagaaa taaagtattt tttagaagaa
     5521 ttaaaaaact attctaccga agaaattatt tataattttg aatttagtaa gttagataat
     5581 gatttaatag aatttaaaga agaaagtgat atatacttaa gttttaaaag aggaatagat
     5641 attataaaac agatattaga atatccaata ataaggttag acctcttagt tttgtttatt
     5701 gggaaaaaac ttagtttaga aaaagaagat aaagctgtac tggattatat atctttttat
     5761 ataaagtatc tagtatcaga aaatataaat atggatttag aagatgtata taatttattg
     5821 tttgatgtaa agaacagggt gtttaatcat ataatagatg tggtatatga gataaatgga
     5881 tatgaaccag agcctggtag tataacacta tgtaactatc ataaatcaaa ggggatggaa
     5941 tgggattgtg tgtttttact aggtcttgtt gaatataatt tccctgacaa tattaatcaa
     6001 aagtttcaga gtgataaatg gtatctaaaa gaaaaatata aaaaccctat ggcaattatt
     6061 aaatcagaag ttgaagctat attaaaagga agtatttcaa cagattatgc tcataaaacg
     6121 aagattgatt ctataaatga aaagattaga cttttatatg tagggattac tagagcaaag
     6181 gaaatgttag tactttcagg aagtgcttat agagatgaaa gtgatatagg gaataagaga
     6241 aaagagcaaa aaccatgtat atatttaagc agacttaatc aacatattat tgaaaaaagg
     6301 tcaaattaat atataataag ttattaaaag attgtttgaa atatatctaa aagaggataa
     6361 aacaatatgt acagtaagta actactaaaa aataaagtta agagagaagt gatatgagtg
     6421 aacgataaat taaaatattt ttcttatagt caaaattcaa ttaacacata taaatcatgt
     6481 ccattgaaat ttaaatataa atatattgat agaataaatt ggaaaaatga tgatgttggt
     6541 agtagagagt attatgaaac tttaaaaaca ggaagagatt ttcatttgat ttgtgaaaga
     6601 tattttagca atatacctct tggaatctat tttaatgagg atgataaaaa tagtaaaaag
     6661 tttttaaagt ggatggaaaa tataaaaaaa gttgttccta ttgaaaaagg aaaaacgtat
     6721 ttgccagaat atgaagtaag gatgacatta aatggggata taatacaggc aaagtatgat
     6781 ttagttgtaa ttggaaataa caatatagaa atatgggatt ggaaaaccga gtctaaaaag
     6841 attgactata aacacgtaga aaatagaata caaactattg tttatatgtt tttagcaaaa
     6901 gaagtaatac ctaaagtttt aaaaatggac ataaatgtta aagatataaa tatgagatat
     6961 tatcaacctg aatttgatga cttaccaatt accattttat atgatgaaga aaaacatgaa
     7021 gcaaatagaa ataaaattca aaattatata agtatgataa aaaacactaa ttatgaagaa
     7081 catacatacg aagacaatct atacaatgat atagaaaagg tatataataa agaagaacgt
     7141 atgtgctata gaaataaaaa gcattgcaag tattgtgaat ttaataagtt atgtaatggc
     7201 aaagaaatag attatagtat tttggaggcg gaaatttatg ggacttaggt ttgtattagg
     7261 tagaagtgga agtggaaaat ccacctatat actagatgag attaaaaaag aagctcaaaa
     7321 aaatgaaacg acatcaatta tacttttagt tccagaacag tacacatttg aggctgaaaa
     7381 tagagtaagt aaactatttt taggaaaaga aaaagataag tatctaagag ttagggtact
     7441 tagctttaag acactaagta atatagtttt ttcacaagta ggaggattga cagatgtaaa
     7501 tataaattct agtggaaaag caatgatggt atatagagct attgaagatg taagtgaaga
     7561 attaaatgta ttttcaaaat caaaatcgca gtctggtttt gtaagctcaa ttacagatat
     7621 gatatcagaa atgaaacaat acaatataag ccctgaaatg ttagaaaata tatctggaga
     7681 attggataat gaaactttaa gtttaaaatt aaaagatata agtaaaatat ataattcttt
     7741 tgaagggaaa ttgcatgaaa attatgtaga tgctcaagat atgttaactt cattggcaag
     7801 taaaattgaa ttaagcagtt atttagatgg agcttgtgtt tatatagatg aatttacagg
     7861 ttttacacca aatcagtaca atgtcataaa gagtatatta aataagtcta aaagtgttaa
     7921 tatctctttg actgtagatg atatcaacta tattgggtac agtaaatctg atatgttttc
     7981 cagaacgaaa tttacttatt caaaattgac tcaattgtgc aacgaagaag gcataaaaat
     8041 attgccacaa gtaaatttaa acacaggtgt tataaaaaga tttgaaaaag ttaaggaatt
     8101 acagcactta gaaagatttt ataatgctta tccatataaa atatattcaa atcaaactga
     8161 aaatataaag ataaaagagt ttaacaattt atattcagag gtggaagaga tagccagaga
     8221 aattgtacat ttagttagag ataagaatgt cagatataga gatataacca ttgctacaag
     8281 agatttaaat agatatgatt ttttagtaca ctctatattt aatgaatata atataccaaa
     8341 ctttatagat aagaagagag aggcaaaaag taatccaata gttattttaa ttatttcagc
     8401 tttagaaatg aagaatagac gatatgggta tgaaactatg tttaggtatt taaaaagtgg
     8461 acttatagga attgataatg atgatataaa tttactagaa aattatgtgt tggctaatgg
     8521 gattaaaggt aaaaaatggt ttgatgaaaa atgggattat agaataaccc aaagtctttc
     8581 aggtcaagaa agtgaatttg aactggaatt aaaagagaaa ataaatgaaa taaaaaatag
     8641 agtattagag cctatagtaa ttttacaaga aaaattaaga ggaaaaaata gagtaaaaga
     8701 aatctgtaga tatatatatg aatttttatt agatataaat atgccagaaa ctatagaaag
     8761 tttaataata aattttaagg ataaagggga acttgatgtt gcaaatcaat actcacaagt
     8821 ttgggacata gtagtggaca tattagacca aatggtggaa cttatgggtg atgaaataat
     8881 ttctttagaa aaatttataa aattaataac cttaggtttt gatgaatatg aattaggact
     8941 ggttcctcct agtatagacc aggtacttgt aagtagtgtg gatagaatga aaaatccaga
     9001 cacgaaatat ttatacctag ttggaactac tgatggtgtg tttccgctga ttacaaagga
     9061 tagtggaata ttgagtgata atgataggga aagtcttgga aacaaaggaa tcgaagttga
     9121 tattgatagt aagactagga gttttgaaga acagtttctt gtatataaag ctcttacttc
     9181 gacatctaag aatttaacca taacatatcc aatatcagac catgaaggta aaacgcttag
     9241 accatcaata attatatcaa gacttaagaa gatatttcca aatatagaaa ataaaagtta
     9301 tttagtggaa gaaaataaaa gcactgataa agatatactt aaaaaaataa cggttaaatc
     9361 ccctacattt aatgaactta taaacgtaat aaaaaattat gacagtgatg gttacaatac
     9421 agaagagatt aatagtattt ggttagatat atataggtac tatttaaaag atgaaatcta
     9481 cagttctata acaaaaaaag ttataaaagg tcttagttat actaatcaag ttcataagat
     9541 tgaagagaaa aaaataaggt cattgtataa aagtaattct cttagcatat ctaggcttga
     9601 aaaatatgct gaatgtccat ttgcttattt tatacaatat ggtctaaaag caaaaaagcg
     9661 taaagagtat tcatttacac caccagattt aggaactttt attcataata ttttagatag
     9721 gttttcaaaa gaacttttac aagataattt aacttggaga gatattgatg aaaaatatat
     9781 agaattaaag ataggtataa tagttgatga aataatttta aagataccag gatatatatt
     9841 aaatagttct gagaggtata aataccttgc atatagatta aagaatatgt tgacaactgc
     9901 tataactata attagtcagc aaataaaaca aggttcattt gaaccaatag attatgaagt
     9961 taaatttggc aacaatggaa aatatccacc tataaaaatg gtgttagaaa atggacaaga
    10021 agtaagttta attggacaaa tagatagagt tgatgaattt gaagaaggag aaaataaata
    10081 tataagaata atagattata agtcaggcaa taagtccata agtctaacag aaatatatta
    10141 tggtctacaa ttacaattac ttgtgtattt agatgcaatc cttgaaagtg caaaggatga
    10201 agatataaat ataaatccag ctgcaatact atattgtaga ataaataatc caatagcaaa
    10261 atttaatgaa gataaagatg atgaagaaat acaagaagca atattaaaag aacttagaat
    10321 gaaggggctt gtagtaaaag actctcacat agttaaagag atggataaat cattaataga
    10381 tggagaaagg aaaaactctt tagtaatacc agtaggtctt actaaggatg gaaatgtagg
    10441 taaaagtact tcagcaataa gctatgagga tttcaaactg cttagaaaat atgtaaggca
    10501 tgctataaag gatttatgtg aagaaatgtt aagtggagaa atacgtatag ctccatataa
    10561 acataaagat ggaacatcat gtgacttttg tgattactct gctatatgtc agtttgattc
    10621 aactataaaa gataacaagt ataaaaattt aaataataag agtaatgaag agataataaa
    10681 aatgatgaaa ggagatgtta attagtgagt tctcctaaat ggacaaaaga gcaattagaa
    10741 gttatagaaa gtagagaatg caatttattg gttgctgctg ctgctggttc aggaaaaaca
    10801 gccgttctag ttgagcgtat aattcaaatg ataacaagta gagaaaatcc aattgatata
    10861 gataagttat tagttgttac atttactaat gctgctgcgt cagaaatgag agagaggata
    10921 ggggatgcta taggaaaagc cttagatgaa aacccagaaa ataaacattt acaaaaccaa
    10981 cttgttttat taaataaatc tagtattaca acaatacact cattctgttt ggatgttata
    11041 aaatcaaact ttcatagaat aaacttagac cctaatttca gaataggaga ccaaactgaa
    11101 tgtgctattt tgaaacaaga agcaatagag gaagtatttg aagatttata tgaagaaaga
    11161 gatgaggggt ttttaaattt agttgaaagt tatgcagaaa gaggaggaga taaagaagta
    11221 caagatatta tcttgggaat atattcattt gcaatggctt ctcctgaacc taaaaaatgg
    11281 ttgattgact cagcagaacg atttaatatt gatgaaaatt ttgatttttc acagtctatt
    11341 tgggctagag ctatattaga tactgtaaaa atagagatta atggtctttg tctaaatatg
    11401 gaaagggcat taaaagaagt ggaatcaata gaagaattag aaacttttgc agaaaagtta
    11461 tccgttgaat ataaaaaaat tgctgacatt tcacaagcat gtaataaatc ttgggatgag
    11521 gcatataaaa agatggcaag tatgtccttt gaaaattatg taaaaggagt aaaaagaatt
    11581 tcaaaagatg ctccttcgta tataaaagaa tcaaaagaaa aggcaaaaac tataagagat
    11641 aagactaaga agtctttaga aagtatagta agtgctacat tcaataaaga taatgattct
    11701 attagagaag aaataaaata tctttataac atagttaaac caatatctag tgttgtactg
    11761 agatttgaag aggaatatag caataagaaa agagaaaaag gaataataga ttttaacgat
    11821 atagagcatt ttgcattaaa tatacttaca gatgtagatg aaaaaggtaa tattgtacct
    11881 tctgatatag cagttggata tagaaacaag ttttatgaaa tatttataga tgaatatcag
    11941 gatagtaatc tagtgcagga agtactgctt aaagctgttg caaatacaga aactccaaat
    12001 agatttatgg ttggtgatgt aaaacagagt atatatagat ttagacaagc aaagccagaa
    12061 ttatttttgc aaaaatataa taactataat gataaaaagg gaagctccca tagaaaaata
    12121 atgctttaca aaaactttag aagtagagaa gaagttgtag atgctgtaaa ttatatattt
    12181 gaaaatataa tgaatgagaa tataggagaa attgaatata cagaaaaaga aagactaaat
    12241 ttaggtgcaa attttaatgt ggatacagac gagaaatcta ttattggtgg agcaacggag
    12301 atacacctaa tacaaaaaga taataaactt gatgatgata ttataaatga taaagacgat
    12361 agaattaata acaaagaaaa tgaaatagag gaagaagaaa aattagataa tatacaactt
    12421 gaggctagaa tggttggaaa cattatcaaa gatttaatga aagtcaatga agatggaaaa
    12481 attcaaaagg tctatgataa gggaatagat ggatatagac ctgtggaatt tagagatata
    12541 gttattcttt taagagctac atctgcatgg gctccagtgt ttgcagatga attgatgaat
    12601 atggatatac caacgtatgc tgatgttgga gtggggtatt ttgatacgat tgaaataaaa
    12661 acaatattgt cattacttca aataatagat aatccaatgc aagatatacc tcttatatct
    12721 gtattaaaat cacctatatt tggatttaca ccagaggatt tgattgatat tagagttcag
    12781 agtaaagata aaatttttta tgaggtttta aaaagtacag cagagtatga tggatttaca
    12841 gattctcaaa atgaaaatga gtctgagttt ataccaagtg aagaatgcat aaacaaaagt
    12901 aaagattttt taattaagct aaaggaattt aaagaaaagt caatgtacat gagtacagat
    12961 gaatttatat ggtacttgta cacaaggact ggatactatg cctatgttgg agcattgcca
    13021 ggtggaagtc aaagacaagc taatttaaaa gtattgtttg agagagcaaa acaatttgaa
    13081 gaaaccagtc ttaaaggaat attcaacttt gtaaatttta tagagaaatt aaaaaaatct
    13141 agttctgata tgggaagtgc aaaaacactt ggtgagaatg caaatgttgt tagaataatg
    13201 agtattcata aaagtaaagg tctagaattt ccagtagtca tatgctctgc aatgggtaaa
    13261 aatttcaata ctcaagattt taaaaagagt attttatatc atcataattt agggtatgga
    13321 cctcaatttg tagactatga gagaaggatt tcttttccaa gtatagccaa agaagcatta
    13381 aaaagcaaaa taaatataga aaatttatca gaagagatga gagttttata tgttgcattt
    13441 acaagagcta aagaaaaatt gattataact ggttctacta gaaatataca agatagtata
    13501 aagagatggt caaatggaat tgaaagttta gatacaatat cacaatacga gatattaaaa
    13561 ggtaaaaatt ttttagattg gataatgcct tgtgtattaa gacataggga tttatctaat
    13621 ctactagaag aagtaggatt agacgcagtt tttaatgttg aacataattc aaaatggtat
    13681 ggaaagttgt ggaataagag tgatatttta gtggaaaaga agagtgatga agaaaaagaa
    13741 agtattgaag aaattcttga aaaaatagat gtagataatc ctgatagtga ttattacagt
    13801 gaaatagaag aaaaattaaa ctatatatat ccatatgaat ttagtacaag aaaacctgct
    13861 actatatctg ttacagaaat aaaaaagata caaaataatt atgaagaaga gttaataaat
    13921 actatatttg aacaaaaagt aatattaaaa aagcctctat ttattcaaaa tgaagaagaa
    13981 agagaaaaaa taagtggaac agaaagaggt acaatagtcc atttagttat ggaggtttta
    14041 gacttaaaaa atgtaagtag tgtaaatgat ataaaatcac aaataagagg ttttgtttca
    14101 aaaggtataa taacagaaaa acaagctagt atagtaaatc catataaaat atataaattt
    14161 tttgcctcta acataggaaa aagaatgttg aatgctgaaa ttataaatag agaaaaatca
    14221 atttatgcac aagtaaatat gaaagacata tatatttatg aaaagcttat aaacaatgac
    14281 gataaaaaac tttatgataa tgaaagtgtc atgttaagag gtatagttga tgcttatttt
    14341 gaagaagaca atcaaatagt tttggtggat tataaaacag attttgtaaa tgaggaaaat
    14401 ataaatcaaa taatagagaa atataaaaaa cagttggatt tatatgcaga tattattgaa
    14461 actttaactg gtaagtcagt aaaagagaaa tgtatatatt tatttggagt tgatgaagct
    14521 gtttgttatt agtttttata tataaattaa acctaaaaga tagaggaagg ggaaaaaatg
    14581 agatttattc atacatcaga ttggcatcta gggaagtccc tagaaggcca ttctagaatt
    14641 gaggaacaag caaagttttg tgaggaattt ataaaaatag tagaatcaaa tgaaatagat
    14701 atggttataa ttgctggaga tgtatatgat acttctaatc caccagcaca agcagaaaaa
    14761 ctattttatc agactgtatc aagacttgcc aataatggtc agaggtgtgt attaataatt
    14821 tcaggaaatc atgataatcc agagagactc tctgcaataa cacctctagc acatgaacag
    14881 gggatactta tatatggtta tccattaagt gctacgatag aagctaagta taaagggttt
    14941 gaaataacat atgcaactca gggatgtaca aaactgaata taaatggaga aaatattgtg
    15001 gttgctacat taccttatcc aagtgaaaaa agattaaatg aagttttttc aagtgaagat
    15061 gagtttgaga aacaaaaaaa ttattctgaa aaggtaggag atatatttag aagtttagaa
    15121 gaaaatttta gaagtgatac tataaatata gccgtatcac atatatttgt aataggtgga
    15181 gagagtactg aatcagaaag acctatacaa ttaggtggaa gttttttagt tgaaagaaaa
    15241 gatttgccag aaaaggctca atatactgca ctaggtcatt tacataaaca acaaaaagct
    15301 tccgaacgtt taaatgcata ctattctggt tctccacttc aatatagcaa ggatgaaaga
    15361 gcgtatacca aaggggcata catagtggat ataaaagctg gtgaaaaacc aataattgaa
    15421 gatgtatatt ttaataatta caaacctata gaagtattta aatgtaatgg aatagaagaa
    15481 gcactagata tatgtgagca aaatcaagat agagagattt ggtcatattt tgaaataaat
    15541 actgatgaga taatatcaca gaatgaaatt aaaaaaatga aggaactatt aaaagacata
    15601 atagaaataa aaccaataat aacatcttgt tatgaacaag aaagtgtaga tataaaagaa
    15661 aagtctatgg cagagttgtt tagagagttc tattctttta gtaaaggtgt agagccaaaa
    15721 ggagaactta tggatttatt tctagatata ataagtgaag aaggtgaatc agcagatgag
    15781 accaattaga ttagaactta cagggcttaa tagctatatt gataaacaag ttatagactt
    15841 tgaaaaatta attgaaagag gtttgtttgg gatatttgga actactggaa gtggaaaatc
    15901 tacaatctta gatgcaataa ctattgctat gtatggaaat atatccagaa atacaaaaga
    15961 atatataaac agtgtgtgcg ataaggcaat tatatcatac gagtttgaga taggaagcaa
    16021 aaacactaga agaagataca ttgtagatag aaccattgct agaagcaaaa caggaacaaa
    16081 gacctctaat gctagattaa tagaagtatt gaatgacaat acccaaaatg ttttagctga
    16141 taaagttgtg gaagtaaatg aaaaagtagc gcaggtcgta ggattaacgg caaatgattt
    16201 tactaggtca gtagttttac cacaagacaa atttaatgag tttctaagat tatcacgtgc
    16261 agatagaaga gacatgttag agagaatttt taatcttgag aaatatggaa gaagtcttgg
    16321 cgaaaaagtg aaaaagagaa aaaatataca gttacaaaat ttaaaagatt tgaaatcaaa
    16381 attaagtcaa tatgatggaa ttacagagga agtatataac aatataaacc aagaattaat
    16441 agagttaaaa aatttggaaa aagataagaa taatgcttta gacctagctc aaaaatctta
    16501 tgaagaaagt aaaacagtat atgaagaaca attaaagtta gaaaaaaatg aacttagaaa
    16561 aaatgagctt gatttaaaaa atagtgagat aaaagaaaag attgctcttg tagaaaatga
    16621 tagtaacgct aaaaaggtag acccatacat aagttcagtt caaaatctag aaaaaaagat
    16681 agatgaagat agcttcgcag taagtaattt agaaaagaaa ttagcaatat taaatcagga
    16741 gcttgaagtt acaaaaaata gatatgaaaa aataaacaaa attaagaatg aagaaatccc
    16801 taaattgagt gaagaaaaaa taagattgca acaagcaata aaattagaag aagagttagt
    16861 tttattagat agagagttaa aagagctaaa agaaagtgga attaatttaa ataagactaa
    16921 agttgaatta gaaaaagtaa agcaggtgtc agagtccaga aaagatgcag ttactaaatc
    16981 tataaaagaa gttgaaggaa agattgataa agtaaatata agtgcagaat taaaacaaaa
    17041 aatatttcta gcatatgaat acgagaaaga ttacaacaac gtattggaag aaaaaaatca
    17101 aaagctaaat aaactagaag aaatactcaa agagacagaa aatataaatt taaaagttag
    17161 atatattgat aaagataaga atgatgtaaa tcgtaattta gaaaatttat cacttcactt
    17221 ggatgttctt cttaaaaaat gtcctggtaa gagtgctgat ttacttttaa aatctgaata
    17281 tgttactgaa ttaagaaata aagcaaataa tactaaagaa aatgaaatta agaaaagcag
    17341 tatacaagat gaattaaaga taatacttga aagtaagttt aatactgaaa gggaagtaaa
    17401 tttactaaat gaaaaacttg aaaataatag aaagaacaga gatgacttag aaaaagaatt
    17461 agaagaacta aagtatttaa atttagcctc tgaacttaga cgtgaattaa aagaaaatat
    17521 gccttgtcca gtttgtgggt caaaacatca tgaaaatcat gatataacta agtatgatga
    17581 aaatatatct tttgtcaaag aaaaactgga gaaattagaa aaagaaaaaa taagtataag
    17641 aaacaatata gaggaattaa atgctaaagt aagtgggtat ttatctatag aaaaaatgaa
    17701 gactaaagaa cttgaagatg taaaaggaaa actagggggg attccatcta gtcaattatt
    17761 aaaaaaactt gatgaagaac aaagaaaact tgcattgtta aaatctaata ttcaggagtg
    17821 ggagaaagaa aaagagagta ctgaaaataa agtaacatta gctaaagaag aaaaaaataa
    17881 aattgaaaaa gaagaattaa aaataagaga gagtctgaat aattataaga aattaacaag
    17941 agatttaaac atagaaatag aaggtttaga aaataaatgt aaaaaactta aacaggaata
    18001 cctaggatta aagacgatta ctaaagtctc taatctattg tcaaaagtaa aagaaataag
    18061 ggagaatgaa aaagaattag aattactaag tactgggcat tcaaatttat taaaaaatag
    18121 agactcacta gatattaaaa taagagaaaa tgagagtaaa ctacatgaaa tagagataga
    18181 attaataaag gctagagaat tatatgtaga aaagaaattg agtagagata ataagtataa
    18241 agaagttatt agcataacga aaggtgattt agctaaaaat cttttacata atgttgaaga
    18301 aaatatatgt aagatattag aacaagaaga atcaagtaag aaaaaattgg aagagcaaag
    18361 attagaatat gaaaaaaaca ttgctgaaaa gcacaatata gatggtagac ttaaaactgc
    18421 taaggaacaa tataaagacc aaaaatcaac actaaataaa ctattggcag aaaataaatt
    18481 tgaaagtata tatgcagtaa aaagagcttt attagatact gatgcagtaa aaaaattgat
    18541 tgaagaaata ctagagcatg aagaagaaca aaagttatta tcatttaaaa taaaatcatc
    18601 aaaagaaaaa ttaaatggaa gaagcataaa gaaggagtac tttgaacagt taaaagatga
    18661 aatttataat ttgaaagtag aaataggaaa tatatccaaa gatattggag ctaatcaaaa
    18721 tcagttaata acattaaaag actctttaga taagataaat gattttaata aacaacttaa
    18781 ggtagttgaa cacaatgtgg acttattgga agaattagat aaatgtgtac aaggaaataa
    18841 atttgtagaa tatgtttcta caaaccaatt aaaatatata gcgttggaag catcaaaacg
    18901 attggatgga ataacaaaag gaagatatgc acttgaaatt gactcaacac taaactttgt
    18961 aatgagagat aactttaatg gaggagaaag aagaagcatt gatactctat ctggtggaga
    19021 gacattcttg acatctctat cattagcact tgctctttca tctcaaatac aattaaaggg
    19081 aagtgcacca ttagaatttt tctttttaga tgaaggtttt ggttcattag atagtgaact
    19141 tttagaaatt gttatagaat ccttggaaag attgcatagt aataatctaa gtgtgggtat
    19201 aataagtcat gtcgaggaac ttaaaaatag agttcctgtt aagctattag tttcatctag
    19261 tgaggctgga ataggttcaa aagtaaaaat tgaatatagt tagaaaatat atgttaattg
    19321 aatatattat ctaatatttc caaataatat gtaattgtga tataataaaa actaatatta
    19381 aagttattta taattaatat taatataaaa acatgtaagt aggagtgtgg taagtgttgg
    19441 aatcgcccaa taatttgatt cagattattt ttttaatagt attacttata gggtctgcat
    19501 ttttctcagc atctgaaacg gccttaatgt cgttgagtaa aatcagaatc agatatatgc
    19561 aagatgaagg agttaaagga gctaagttag taagttcatt aatagaaaat ccaaataagc
    19621 ttttaagttc tatattggtt ggaaataacg tcgtaaatat agcagcaaca tccatatcaa
    19681 catctttgtt tataggatta atgggagaaa aaggagtggc acttgctaca gctgttatga
    19741 cagtattagt attaatattt ggtgagatta ctccaaaaac aatagcagca aataattcag
    19801 aaaaggtttc tctattagta tctaaaccta taaaagcaat tatatttata ttaagaccaa
    19861 tagtatggat atttaatata attacaaata ttatatttaa attatttggt ataacaaata
    19921 aaggagctaa atcattcata actgaagaag agctaaaaac tatggtaaat gtaagtcatg
    19981 aagaaggcgt ccttgaaatg gaagagagag aaatcataaa taatgttttt gaatttggag
    20041 atatgcaagc taaaaatgca atggtacaaa gaatagatat ggtagctata gatatggaag
    20101 atagttatga tgagataata caggtattta aaactgaaaa attaagtaga atgcctgtgt
    20161 atgaagaaac tatagatgat attgtaggaa tacttaatat aaaagatatt atatttttat
    20221 ctgatgaaga aatagagtca tttgatatta aaaattatat gagagaacct tttttcacgt
    20281 atgagttcaa aaagataaca caacttcttg aagagatgaa acttgaaaaa agtcagatgg
    20341 ctatagttgt agatgagtat ggtggaacat ctggactttt aactatagaa gatttagtag
    20401 aagtcattgt tggagatata gaggatgaat atgatgaaga agaggatgaa atacaagtta
    20461 taaaagaaga tgagtatatt gtagatggaa gtactaaaat tggtgatgtt aatgaactta
    20521 ttggtgtaaa cttggaatct gaggagtttg attccatagg aggttttata attggacacc
    20581 taagcagatt gccagaagaa aatgaagtaa ttgaagttga taatataaga ttttgtatag
    20641 aaagtataga aaaaaataga attaaaaaaa taagaatata tacataatgt aataatgttc
    20701 ttaaatttat
//
LOCUS       DAMJVI010000067        20478 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.069, whole genome shotgun sequence.
ACCESSION   DAMJVI010000067 DAMJVI010000000
VERSION     DAMJVI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20478
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.069"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(104..1093)
                     /locus_tag="PC545_002462"
     CDS             complement(104..1093)
                     /locus_tag="PC545_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDF2457229.1"
                     /translation="MLKEEKTYQEKDAKVFNGVAALIMILLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLILGLVFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAVSRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            1533..2288
                     /locus_tag="PC545_002463"
     CDS             1533..2288
                     /locus_tag="PC545_002463"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDF2457230.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELELVCGVARSADEKGHHS
                     DTKLYKTMTDVTEKTDVIIDFSHHDTLEPILSYALKNNTPVVLATTGYDENDLNKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIREVLPESENTHGRYGRDCKRKPNEIGIHSIRGGNIISDHDVVFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLIGKQSGLYKMSDILG"
     gene            complement(2642..4318)
                     /gene="bclA2"
                     /locus_tag="PC545_002464"
     CDS             complement(2642..4318)
                     /gene="bclA2"
                     /locus_tag="PC545_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HDF2457231.1"
                     /translation="MSDISGPSLYQDVGPTGPTGATGPTGPTGPRGVTGATGANGITG
                     PTGNTGATGANGITGPTGNMGATGPNGTTGSTGPTGNTGATGANGITGPTGNTGATGA
                     NGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLTGAT
                     GATGANGITGPTGNTGATGANGVTGATGPTGNTGATGPTGSIGATGATGTTGATGPIG
                     ATGATGADGEVGPTGAVGATGPDGLVGPTGPTGPTGATGANGLVGPTGPTGATGANGL
                     VGPTGATGATGVAGAIGPTGAVGATGPTGADGAVGPTGATGATGANGATGPTGAVGAT
                     GANGVAGPIGPTGPTGENGVAGATGATGATGANGATGPTGAVGATGANGVAGAIGPTG
                     PTGANGATGATGATGATGANGATGPTGATGATGVLAANNAQFTVSSSSLVNNTLVTFN
                     SSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVTQNNFIAK
                     AVNTLTSSDVSVSLSFLVDARAAAVTLSFTFGSGTTGTSAAGYVSVYRIQ"
     gene            complement(4696..5235)
                     /gene="hpt"
                     /locus_tag="PC545_002465"
     CDS             complement(4696..5235)
                     /gene="hpt"
                     /locus_tag="PC545_002465"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2457232.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLRELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(5563..6870)
                     /locus_tag="PC545_002466"
     CDS             complement(5563..6870)
                     /locus_tag="PC545_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDF2457233.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDAAEKAESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(7295..9070)
                     /locus_tag="PC545_002467"
     CDS             complement(7295..9070)
                     /locus_tag="PC545_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457234.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKELFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDSITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLRERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVKSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(9067..9204)
                     /locus_tag="PC545_002468"
     CDS             complement(9067..9204)
                     /locus_tag="PC545_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457235.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(9282..9851)
                     /locus_tag="PC545_002469"
     CDS             complement(9282..9851)
                     /locus_tag="PC545_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2457236.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(10035..10670)
                     /locus_tag="PC545_002470"
     CDS             complement(10035..10670)
                     /locus_tag="PC545_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDF2457237.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(11338..12219)
                     /locus_tag="PC545_002471"
     CDS             complement(11338..12219)
                     /locus_tag="PC545_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2457238.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWINVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(12339..13346)
                     /locus_tag="PC545_002472"
     CDS             complement(12339..13346)
                     /locus_tag="PC545_002472"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HDF2457239.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(13366..13848)
                     /locus_tag="PC545_002473"
                     /pseudo
     CDS             complement(13366..13848)
                     /locus_tag="PC545_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
     gene            complement(13866..14333)
                     /locus_tag="PC545_002474"
     CDS             complement(13866..14333)
                     /locus_tag="PC545_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HDF2457240.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(14348..15106)
                     /gene="prdD"
                     /locus_tag="PC545_002475"
     CDS             complement(14348..15106)
                     /gene="prdD"
                     /locus_tag="PC545_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HDF2457241.1"
                     /translation="MEEKIMRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKDQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKVRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(15186..15911)
                     /gene="prdB"
                     /locus_tag="PC545_002476"
     CDS             complement(15186..15911)
                     /gene="prdB"
                     /locus_tag="PC545_002476"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15459..15461),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HDF2457242.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(15949..16233)
                     /locus_tag="PC545_002477"
     CDS             complement(15949..16233)
                     /locus_tag="PC545_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457243.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(16314..18194)
                     /gene="prdA"
                     /locus_tag="PC545_002478"
     CDS             complement(16314..18194)
                     /gene="prdA"
                     /locus_tag="PC545_002478"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HDF2457244.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(18623..20383)
                     /gene="prdR"
                     /locus_tag="PC545_002479"
     CDS             complement(18623..20383)
                     /gene="prdR"
                     /locus_tag="PC545_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator
                     PrdR"
                     /protein_id="HDF2457245.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSDQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISEKVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKDVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKIDVSKI"
BASE COUNT         6054 a         3938 c         2424 g         8062 t
ORIGIN      
        1 ttataattca ataaagcaaa ccattaatct ttatataatt taataaaatg aactactaaa
       61 ttttctatat atcaataaaa taaactatta aactttttat aatttaataa atagaactgc
      121 tgtttaccac tggttgtgca tctttattgc cacaaagcac aactaacaga ttacttacca
      181 tagctgcttt acgttcttcg tccaaagtca caacattatt ttcacttaaa ttagtcaatg
      241 ccatctctac catacttaca gccccctcaa caatcttttt acgtgctgct attatagcct
      301 ctgcttgttg acgttgtaac attgctgctg caatctctgg tgcataagaa agatgagtaa
      361 ttctcacttc acacacttca attcctgcaa tgtcaaccct tgattgtaat tcatctttta
      421 atctatctgc tatttcctga ctactaccac gaagagattt ttcatctcca tcttcagata
      481 catcatatgg ataaagtctt gaaacattgc gaatagtaga atcacactga atagataaga
      541 atgtattata attatccaca ttaaatactg cctttgttgc atctataacc ttccaaatta
      601 caaccactcc tattataatt ggatttccca ataaatcatt gactttctgc ttttcattat
      661 taagagtcat tgtttttaaa gatacttttt tgccccttga attagttgat atatctacct
      721 tttcatcaga tgaagatttc gatacattaa ttcttgatac tgctgggttt attgctgaac
      781 aaaatggatt cacccaatag tacccttctt tttttatagt tccataatag ttaccaaaca
      841 aaactagaac tatagcttct ttaggattaa tcatttttag cccaaaaaat aggataaaat
      901 ctactactat aaatacaagt cccaaaatta acataagtga acctattcca gtaaatccac
      961 tatcaagttt tactatccca aaaaccatga tacctattga aactactaaa aataataaaa
     1021 tcattatcaa agctgccact ccattgaata cttttgcgtc tttctcttga taagtttttt
     1081 cttcttttaa catatctatc actcccatct cagtatatta agttaagata tttctaattt
     1141 aataataaca cttttaacaa ttaaattgtt gaatttaaaa ttaatactat atgtttatat
     1201 attttttata catctttatg catacatgta tttagttatt ttaatgtatt aatattaatt
     1261 tccaaataaa tcatatgata tatgaatatg gtatttcaaa attaactttt tagttcatgg
     1321 ggcactcttt ttggtatgaa gtcaaatata tttttatcat ttaaacaatg aaaaagaaat
     1381 ttatctatat tttgaaaaat ccacttaaat tttttaatgt aagataaatt gtaagcttat
     1441 tatcactaat attattttat ataaattata ttagtgataa aataaactta tagaaatata
     1501 acaaattttt attaatttca taggaggaat ttatgttgaa agttataatt agtggctata
     1561 atggaacaat gggaagagtt ttagcagaat gtgtaaaaaa agataatgaa ttagagttgg
     1621 tttgtggtgt agctaggtct gctgatgaaa aaggtcatca tagtgataca aagctttaca
     1681 aaacaatgac tgatgtaact gaaaaaactg atgtaattat agacttttca catcatgata
     1741 ctttagagcc aatattgtca tatgctctaa aaaataatac acctgtagta ttagctacta
     1801 ctggatatga cgaaaatgac ttaaataaaa tagaagaagc atcaaaaaac attcctatat
     1861 tattatcatc aaatacatct cttggagtaa atatattaat aaaattagtt aaggatgctt
     1921 caaagatgtt agaaggtttt gatattgaaa tcatagaaaa acatcacaat agaaaagaag
     1981 acgcccctag tggaactgct actatgatag ctaatgcaat tagagaagtc ttgcctgaaa
     2041 gtgaaaatac acatggtaga tatggtagag attgtaaaag aaaaccaaat gaaattggaa
     2101 tacactctat aagaggtggc aatataatca gcgaccatga tgttgtattt gcaggtgata
     2161 atgaagttct tacattaagc catcattctc aatctgatga agtatttgca aacggctcta
     2221 tagttgcagc taaattttta attggtaagc aatctgggct ttataaaatg tcagatatat
     2281 taggataagt tttgtacata ataaatctca atctatttag gtattcatat tcatcaattt
     2341 gaatatctaa ttgattgaga tttctattct aaaagtttat aaattttatg cttacctttt
     2401 aaatataatc atttttctta aatattatct tatttattgg tatatgaaat catattgttt
     2461 attattttat agtcttttca tcatataaac ctattaaata ttatcctatt tattggtata
     2521 tgaaatcata ttgtttatta ttttatagtc ttttcatcat ataaacctat taaatattat
     2581 tctatttatt ggtatatgaa atcatattgt ttattatttt ataatctttt catcatataa
     2641 actattgtat tctataaact gatacatatc cagctgcaga agtacctgtc gtgcctgaac
     2701 caaatgtaaa acttaaagta acagctgctg ctctagcatc aactaaaaag cttaaactta
     2761 cacttacatc tgatgaagta agtgtattta ctgcttttgc aataaagtta ttttgagtta
     2821 ctccattaaa gttagtagta attgacataa atcctgcaag tgaaagtgtg gcacgtatac
     2881 cgaaagatac attgtatatc cctccaactg caagatttat agtactactt gttggaaaag
     2941 ttatattagt tccatttata aatgatgaat taaatgtcac taatgtatta ttcactaaac
     3001 ttgaagagga cactgtaaat tgtgcattgt ttgctgctaa cactcctgtt gctcctgtcg
     3061 ctccggttgg acctgttgct ccatttgctc cggttgctcc tgtcgcccct gttgctcctg
     3121 tcgctccatt tgctccggtt gggcctgttg gtcctatcgc tcctgctact ccatttgctc
     3181 ccgttgctcc tactgctcct gttgggcctg ttgctccatt tgcccctgtt gctcctgtcg
     3241 ctcctgttgc tcctgctact ccattttctc ccgttggacc tgttggacct attggacctg
     3301 ctactccatt cgctcccgtt gctcctactg ctcccgttgg gcctgttgct ccatttgccc
     3361 ctgttgctcc tgtcgctcct gttggaccta ctgctccatc tgctcccgtt gggcctgttg
     3421 ctcctactgc tcccgttgga cctattgccc cagctactcc tgttgctcct gtcgctcctg
     3481 ttggacctac caaaccattt gctccggttg ctccggttgg gcctgttggg cctaccaaac
     3541 catttgctcc ggttgctccg gttgggcctg ttgggcctgt tggacctacc aaaccatctg
     3601 gacctgttgc tcctactgct cctgttggac ctacctctcc atctgctcct gttgctcctg
     3661 ttgctcctat tgggcctgtt gccccagttg ttcctgttgc tccagtcgct cctatacttc
     3721 ctgttggacc tgttgctcct gtatttcctg ttgggcctgt agcacctgtt actccatttg
     3781 ctccggttgc tcctgtattt cctgttggtc ctgttattcc atttgctccg gttgctcctg
     3841 ttgctccagt tagtccggtt ggtcctgttg ctcctgttgc tcctgtattc cctgttggac
     3901 ctgttattcc atttgctcca gtcgctcctg tatttcctgt tggtcctgta gaacctgtta
     3961 ttccatttgc tccggttgct cctttgtttc ctgttggtcc tgttattcca tttgctccgg
     4021 ttgctcctgt attccctgtc ggacctgtta ttccattcgc tccagtcgct cctgtatttc
     4081 ctgttggtcc tgtagaacct gttgttccat ttggtccagt cgctcccata tttcctgttg
     4141 gacccgttat tccattcgct ccagttgctc ctgtatttcc tgttggtcct gttattccat
     4201 ttgctccggt cgctccagtt acacctctgg gccccgtcgg tcctgttgga ccagtagcac
     4261 ctgttggccc tgttggacct acatcttgat ataaacttgg acctgaaata tcactcatat
     4321 aattaatcct ccttttttaa agttagagta atactctata aatattgagt attactctat
     4381 ataattatat gaatcgacaa tttcttatgt gataatgtat ctatttgctt atatcaacat
     4441 atatgctttg attcttaaat ttcattactt ttttgtaaaa tcttttacaa ttattagtac
     4501 tcttttacaa ttattaatac aattaaaaaa ctcatttccc atcaattatt taaaattttt
     4561 ttattatgct ataaaaaata gataattaaa ttgcataaaa ttaatttaat actactttaa
     4621 ttacttttta aataaaaaaa cctcaacacg attaataaaa atcatattaa ggcatccaca
     4681 aaattatata attgcttatc tatcttcatc agtaacattg aatatataag gaacatttct
     4741 ataataatca ccaaaatcaa acccatatcc aaccacaaac ttatcttcta tttcaaatcc
     4801 acaataatct ggcactaaat cgacttttct tctgcttggt ttatccaaaa gaacacagca
     4861 ttttaaacta gcaggatttt tagcttttag atgttttaac acaaaatcca tagtaagtgc
     4921 agaatcagta atatcgtcta caaccaaaac atcatatcct gctatatcag tagtcacatc
     4981 tgaaactact ttaacctttc cagtactttc ttcattgttt ccataactag atgtagtcat
     5041 aaaattaact ctaagtggta agtttatatt tctaactaag tctgcacaaa atataaagct
     5101 accttttaat agagaaacta ccataagatt tttatcttta taatcttttt ctataattgc
     5161 tcctaattcc cttaatttat ctttaatttt ttcttctgaa tacaatactt cccatttttt
     5221 tgtttctata tccataaaaa gacctccaat tgtaataatc cttcttaatt ttataagaga
     5281 ttgctcactt tttcaatact cttattcaat attagtcaat attattgagt ttttatgttc
     5341 attcaacaat tattaacaat gtatcttata ttttaagtag ttcatatatc cacactaaat
     5401 tacatactat atattttaga caaaaaaaga aaaaacttgc attaacaagt tttttctatt
     5461 atacataaat ttatacataa tcatatttta ttagagggta ctaattaaat ttatttatgc
     5521 tatttttgtt atacctcata atacaaaaca tattataaag tactagtcaa ctttattcat
     5581 atcattcatt acatttaaga ttatctcatc tgttatagac atacctttgt cacttactct
     5641 acctaagttt tgtatacttt gctcaacact tgaaccaact ataccattta gtggtggtac
     5701 aaaacaatct tgcttagcta ttatagcact ttgtatagct gcagatgctg cagaagataa
     5761 ttttaatgca caaccagctt tcgcaccgtc acatatcatt ccacttaagt cagctatcat
     5821 attttctata gctccttcta tttgcttttc agtaccattc ataagccaag agataccagc
     5881 agttgctcca gtagaagcag caacaccaca tccacataca gcagataatc ttcctgtata
     5941 gttcttaata taaccagtta ctagatgaga tatagctaaa gcttttgcta gtctttcatc
     6001 agattgtggg aattttttat tataagcaac gattggaaga attgctgtta atccatgatt
     6061 tccacttcca tttgaactca taacaggcat ttttacccct gccattcttg catctgaagc
     6121 tccagcagtt aacatcattg cataatttat aacatcatta cctaaaagac cttcttctat
     6181 agacttttta attccatatc ctacaccaat accagttttt tgttttaatc catattcagc
     6241 catctcttca ttcattttta ctccatctag taaaaattct atatctttaa aatcaattcc
     6301 ttctacattt tttattaatt cttgtatagt tactgtatcc attaaacttt cagctttttc
     6361 tgccgcatca tttgaagctg atacttttgg ttcattgtct aataatactt ctccatcttt
     6421 ttctaagaac gtaaaattat catgtttagt tcttattctc actttagcaa tatgattttt
     6481 tccttttaac acaacttcta taaatacttt ctctcttgta tcagcaggtg ttattgatag
     6541 tggagtatta tccatataat cctcagctat tttaacttcc tcttttgtta gagtttctaa
     6601 cacacttaat ccattttcag aatgtccacc tactatacct agagctgctg caatttttaa
     6661 tcctaatctt tcagtacctg gtatacctac gcacatacca tttttataaa tactaggact
     6721 aactaacata cgattttcaa ctatttcttc tcctaataat tcctttgcct ttgcacatgc
     6781 tagagctaag gctactggtt cagtacaccc tactgctggt ttaacttctg cctttaacat
     6841 ttccactaat tcttttctta tatctctcat tatatataaa cctcccaatt aagtttagat
     6901 ttagtactca aataattaat taaataattt cttatctata tttatagcaa aatacatgcc
     6961 atatttttta tatcttttta tatttttagt cttatattct ataaccattg gtatgactgc
     7021 ctctatttat gtattttttt tatttgtata gagtatttat attctttaaa atggagaaac
     7081 atatgacaat ttcgcaaaaa gacaaaaaaa agtctcaaaa tgagattcta tatcattttg
     7141 agacggctca aatatattat tttttaactt ataaaaacca ttttttaatt cataaaaaaa
     7201 gattctgcaa aacacttata gtataagttt aaagctttct ttaaagttat gaatttagaa
     7261 aaatttaaat tttatatagc tttttattat attactatga aattatattg tatttttcaa
     7321 tttttctata taatgtagct cttgatattc caagtgcttt agctgttaat tctttatctt
     7381 ttttataatc tttgtactta tttaaagctt ttattatctc aatcttctct agttcatcaa
     7441 gtggaattat cctatcgctt ttaacttcta actcttctac agaggtattc ttaatttttt
     7501 gaggaattac attcattgta attattgaag aagaagacat attcacacta tattcaataa
     7561 tattctgcaa ctctcttata ttccctggcc acttatattc catcatagca tcaacaaccc
     7621 tgctatctat gtcaataaca tttttatcta atttttttga atactcttta accatatgct
     7681 tgattataag tgggatatct tcttttctct ctcttaaact tggtaatttt ataggtataa
     7741 catttaatct atagtataaa tcttctctaa aagtgccatt ttgaaccatt ttttctaaat
     7801 ctttatttgt agcagcaata attcttacat ctattgaaac atttgattta cttccaattt
     7861 tattaagttc cttttcttgt aatactctca ataactttga ctgtaaatgc aaactcatat
     7921 ctcctatttc atctaaaaat atacttccct tatgagctac ttcaaacatt ccaagctttc
     7981 cacctttttt agctccagta aaagctccct cttcatatcc aaataattca ctttctaata
     8041 aattgtctgg tatagcagca cagtttacag ctataaaagg attatctact ctattgctat
     8101 ggttgtgtat ggctcttgca aatagctcct tacccgttcc actttcccca gtaataagta
     8161 ctgttgatgt tgatttagaa gctattaggg cttccttttt agaatttctt atagctacac
     8221 tttctccaat tatgttttcc aattttattc tataatcttt attcatctta ttgtagtttt
     8281 ttatagcatc tcttttatct ataaaatcaa taacatatcc ctttaactca ttttttaata
     8341 ctattttatt gatgttgtat attcccttcg aatcatatcc gtatctttta taaaaaaaac
     8401 tacaggattt gtctttctta aagttattta tagaaggttt ctttataaaa tctaattcga
     8461 caaatatgtt tttgccagtt atactatcct ttatattgaa aagctcttta aattttgaat
     8521 tatatttatc aatttttcca tccacatcaa tagacacaac agctttgtcc atattgttta
     8581 acagtgtctc taattttttc ttttctaatt caagctcata agttttaatt tgagctttta
     8641 atttattaga tattaagtct gccattttgc ctaaaaagtt tataagacca cttttattat
     8701 tttctataat ctgtgcttgt tcctttgtaa aggctataag gcctataaca ccatgagaga
     8761 ctccatcaca tataatggga caacacacct ctgcaaactc tacacaagcc ccacttttat
     8821 agcattccct acatatatca ctactctttg ggtcatcaat tagtattgta atctgctctg
     8881 taagtgattt tttaaaagcc gtatatttat ctactttctc acctattttg tctatatata
     8941 ctcctgtacc tgcaatccta aataactcct tatttactat agttacatct atattgagta
     9001 ctgatactat agcttcagct atattttgaa tatcagattt tatatattct aaacctatca
     9061 taatcatcat tccttatttt tctttaacat aagtatttgt cttatttctt taagaatcaa
     9121 gtatatctta tttaaagtaa attggattga catcaggatt acaaagagca taacaattat
     9181 tattaactca taatttattt ccatctaaac atctccttat aacaaaagac taggcttaac
     9241 ctagtctttt atattatatc catatttata ttttaactca tttatcttct ttttcccaca
     9301 taaatatatt ttaatttatt tgactcttta atcataccat caaagtttaa tcttgaaaat
     9361 atattaaata cttcatgtgg atagtgaatc tttgcaacag aaccacaaaa tttatgttta
     9421 agatagttta acttaacttt taaattcttt aaatttaaat cctctaatat aacctctcca
     9481 ttgcccttta acactctttg tatttcagat gaagcttttt cacaatctgt aataacatta
     9541 aattggtcaa ctattattat cttgttgaac tgattatcat caaatggtag aaactctaac
     9601 ttacctttaa ctacattgca attttcatta tacactagag aataacagac atcttcataa
     9661 ttactattct ccagtatagt tacagaagtt ccaagacctc tcaatctctt gcctaggttt
     9721 cctatatgac caacaagcaa tattttatca cctacattat actcaattgt atccaaaaaa
     9781 cattctaatt catcagatgt atatttagct ttaaccggct gtttatttat ctgacataat
     9841 atgcttttca caatgcttca cctcttcatt tcctcaatta taaattatgt acagattaca
     9901 ttataataaa tggcgagcaa ctattcaaga gatatttcat tagtattttt ttaaacaatc
     9961 tttatacatt ttaatatgat attataccaa atttattagg attttattca taaaaatcag
    10021 tctatttttt atgtttattc attgttattt tcatccaatt tttttatttt cgatatggat
    10081 acttcatatg ccattttttt gaccacatta ttttcatcta tttttttctc atattctcta
    10141 ctttgaaccc taccccaaag ctgtattcta tcaccaacct ttagattctt tgcaaattta
    10201 gcatttctac cccaaactat tgatggtata taatcagatt tattataagg tctgtttata
    10261 gcaactaata aatcggttat ttcccttcct aaaggtgtct tcctatacac aggttcctta
    10321 catacatatc catctaaaaa tatactatta gggtctttat tttcctcgtc tatgcttttt
    10381 atttctctga caaacactgt aagcactaat ctatttttat tatttaatgt tttgttatat
    10441 gatcttaatt ggcctacgac attgactaaa ttttgtttat caaggtcaat atcttggaga
    10501 agtctttctg atacagtcat tggaagtata tcaaaagaat cgcttagtct gtttattttt
    10561 attttcatat tataaaattt ttctccaaat atctcatggc taaattctag cttattgtct
    10621 aactcccctc ttaagtttac tacgttattc tcatctttaa cagctatcat attttatccc
    10681 tccacaattg ttaattctta tatttctggt atcagcttat ataataaata tatttataag
    10741 ggatgtattt tattacaaat ctttgattgc tgtttaaaag tttttattta gttataaatt
    10801 tcatttaata tttcactttt ataattataa aattattatc tttttgtacc acatatacta
    10861 attaaactac ctatttcagt tttgtattaa ttataaaaat cctattatat agttaataag
    10921 tcatatataa cttaaattta ttcactctat ataagcaact tcaacgaatt aaatttattc
    10981 aaataattca gatgaatatt gagtcattat aaacttattt ttcttaatta taagttatat
    11041 tagacttatt ataggttata tttttattaa tctgtattaa tatatgaaca tttattgaac
    11101 ttattgatta taaaatttaa atatttagct ttaaattatg ctttattctt taaaaataca
    11161 acgtttttta cattaaatct actaaatcac tatatatatt acataattgg catgttaatt
    11221 gctttatatt aatttagtaa ataaaaaact gccctaaaat attaattcaa tattttaaag
    11281 cagtttataa tctttattat tagctgtaat atttaataaa cactaagtag tctttaacta
    11341 agcagtcttt ttgtttttat ttgcagccat tatcattgta gcagaagtat aagctataac
    11401 tactattact aatgctttta atatatccat tggtagagtt tttactatat atgcagctac
    11461 gaaaactcct attaaaccac atatagctat tataactgat gcttttttag tatatgcatc
    11521 ttcttttatg aatttcatac ttgcaactgg cattaataat gcacaagaac ccataactat
    11581 tgggaatgca acttttgcag acattcctaa gaaataaacc attgccatac ctggagcata
    11641 taaacctata ccagctgtca ttaaagctcc tagtataaag ttacctatta cacctattac
    11701 taatttaaca ccagtaagac ctaattcatt tcctccacct ggtaatacgt taatccaagg
    11761 atgacctaat aacattaata ttgctgttaa tgctaaagtt acacccataa caagttgtat
    11821 ttttctctcg tccatcttag ctattattcc tgcacctata tatgaaccaa ctgctgctgc
    11881 cacaaccata gatactaaag taacaccatc aactggtatg attgatgtaa atatgaatgc
    11941 ttctgtaaca actggaatag tatgtgctac gtttaatgta ccaggcattt ttttatctgg
    12001 tatatttaac tttaaagcct ttgacattgc tattgttgga gcaaatgacc ctattcctaa
    12061 tgtatcaaag aagtctgtta taaatcctgt tacagcaact tttggagtac tttgttcttc
    12121 tagtttcccc tctttgtttg ctttagctac gtctttacca aacacaactg caaagattgc
    12181 tgcgaatact acatataatg cctttaagat tgctaccata atccatcctc ctattttttt
    12241 attttttcat atattctgaa tcttatagcc taaaagctat agaatagcaa ccgacaagtg
    12301 gctactattc tattttaatt tcctaattta tttattactt atttaagaat aaatccatgt
    12361 ttaagtgggt cttcttcatc tattacaaaa tgattaaatc cagttatata agcagaacca
    12421 gttattttag gtactacagc attgaaatct gcaactttag tttcttcaac tatttcacct
    12481 ttgaataaag ttcctaatat actttcatat acgaattttt ctcctacttt taattcacct
    12541 ttagcatgta aagtagctaa tttagcactt gttccagttc cacatggaga tctgtcaact
    12601 tgaccttgac caaatataac tacatttttg taagtagctt ctggatgagt tggctcatca
    12661 tatatttcaa ctaagtctac agtttttata tgagctaaag ttggatgttg tatttctatt
    12721 ttctcattta ttatatctct aagtttcata gctagttcag ttaatttacc agcattttga
    12781 ggttcgattt ttaaacctaa ttgacttgca tgtattatag caaagaagct tccaccaaat
    12841 gatatgtcaa atttaacagt tccaacacct ggtaagtcaa cttcaacacc ttctttatat
    12901 aagaaagctg gtacgtttaa gaatgatact tctttagctt ttccatctac aactgtaacg
    12961 tctcctctta ttattcctgc tggagcttcc ataactacat gagttacagg ctctactgct
    13021 ggaactacac ctgtttctat agctgctgtc atagctccta ttgtaccatg gccacacatg
    13081 ttaaggtatc cgccaccatc catgaagatt attccaaagt cagcatctgg gcaacaaggt
    13141 tgagtcatta cagaaccaaa catatcatta tgtcctcttg gctctaacat tatagcagtt
    13201 cttaaataat ctagattttc ctctaaatat tcttttttct caggcataga atttcctttt
    13261 atgttaggta ttccacctac aactattcta gttgcttcac ctgctgtatg agagtctata
    13321 gcttgtatac ttctgctaaa tttcatatca aattccccct taggtttatt ttgcgtcttt
    13381 acgcattttt aattttagga aagccaatgc ttttttaaca tcaacttcat ccatgtgatt
    13441 gtctccaact acttctgttt ctatgtagcc tgttgtcttt ttataatcaa ttatggtagc
    13501 tttatccaac tatggattgg taattactaa tccatctgct cccataatag taagacctac
    13561 tgtaggtata tctgatttta caaactctgc tatagcattt gtaaaatcaa tgtgctggtt
    13621 tcctgccata tctaaagtta taatcactcc atcaggtcgc aatgattcaa tccatcttcc
    13681 aagtctagtt gctaaaaatg ttttccattc attgtgctct gatgtcccaa ctacaaccac
    13741 tcctaacaag tctatgtcag aatcattatc tatcaaatct agtaacgggt ctctaaatat
    13801 atgaagtgat gtgtttttca ttgatgtaga aattcccaca aaactcattc ccttacttaa
    13861 attttttatc catgttttct cattttaagt tttaaaaatg ccaaagcttt tttagcatct
    13921 atttcatttg tgttgttttc tccaactact tctgtctcaa taccttcttt tgatttgttc
    13981 atatcaacta tggtatccat atattgattg cttacaacaa acttagcttg agtcccatta
    14041 aatgtaactc caactacagg tatatctctt tttcctattt cctcaaatgt gttagcgtag
    14101 tcaacatgtg agtttcccca accatctgat gaaactatag ctccatctac tctcatggct
    14161 tccagccaag cggccgttct ttgtcccaca aaggttttat tttcatttcc atctggagtt
    14221 cctactacta ttactccaag taaatctaca tccttatcac tttcaactat gtctaaaagt
    14281 gggtctctaa agtgatgaag agatgtttct tttgttgaag gtcctattcc cataatatcc
    14341 acctctctta agtcatagct ctaagggcac catctctgta ttcattagga gatataatca
    14401 taggtacatt tcccatatct ataattgatg ttcctccttc aaatccacta ggttcctttg
    14461 agaataactg attgtcatac atagcacctt gacctgctat ttgttttact atgacaacct
    14521 tttttgcatt tggtcgaact ttgtcaaaat attcacgaac ttgagtagca tttcttccgt
    14581 cttccatttt caaagacgct cttatttctt gtataaaatc atcacatgct ctgaatgctg
    14641 caagtggaag tgttctttca aaaggtaaac ctccctttaa agttacatct acatggatta
    14701 tataatcttc aactgatgga gttccatatc tcccaaatac catttggtct ttcaaaattc
    14761 cttctgaaga accaaattca tgcatttgat ttccatcttc atctgcacca gttaacatta
    14821 catatacacc cgttaaagta tgtgtaatac cctctcccag tctgcctaaa acttttgttg
    14881 atattggtat aatgtccata atagtattaa tttctctgtc ataatctcca ggttttataa
    14941 tgtctacttt tatgtctgtt attaactctt ctctttcttt tatttcatct atacttgata
    15001 aatctattgt aagtacatct ttctttatag aagtttttga accgaaatta acttcattca
    15061 tatgaaacgg ttttataaca agtcttctca ttattttttc ttccataata tccacctttg
    15121 ttcttaaaac ccctctccta accgtgtagg agaagggttc tatattttca aatataaatt
    15181 acattttaaa cgtgagcttt atattcgtat ggtaatggaa ctatcttacc agcagatggt
    15241 attgcaacta attgctctaa tgtatctctt aatatatgtg tttgcatttc tatattatgt
    15301 ggttcaccag cattagcacc cattggaact aatggagcaa ctgctctagg agttccgttt
    15361 tgtctaacta ctggaggaag agctgctatt attattgtag gtattccagc ttcctctatt
    15421 gctctctgca cgatcacggc agttctatgg caagtacctc agccagctgt cataactaca
    15481 ccgtctactc cctcttcctt aagtttagca gcgatagcag gaccagtttc ttcagtgaag
    15541 acatgttggt ttcctccacc acccatgaat gcatagtgca taggagctac tcctttgata
    15601 aatccttctg cagctaattc atgtaatcta tctataggga acatacagtt tatatctttg
    15661 tttacatctc cattgtcata tccaccatgt gataccatca tttcatcaac tgtagctgta
    15721 ttaggtatag ctctaaatgt agtatctcct gctagattaa atctcttgtc agattttaaa
    15781 tgaacaccag ctgctgtagc taaagctata gtcatatctt ttaattcttt agttacagga
    15841 gtccaaactg gaggaggtgt tattggaacg aatatttcag attgaagtcc ttgtactgtt
    15901 gtaaggctca tatatttcct ctcccttcta ttttcaaacg attatttatt atttttcttc
    15961 ttgttttgat tgcatttctg cttgtttttc tttttctctc ctaatatttt ctacatactc
    16021 ttgattaggc tctggactta atacctcagg atagcttagc atgaatccaa cttcttgacc
    16081 tatcttaagt tcgtagtcag ttataagcat tctattaggt attttaaatt gtccaagtct
    16141 accttttagg tctatagtca ctccaccgcc tttgatgtca actataatac cttcataaaa
    16201 agtctctgat gagatatatt ttaacctatc cattataatt ctacctctct atactgtttt
    16261 aaacttggat ctaaatttat gttattgtct aaatcttagt tagtatctaa attttagttt
    16321 tcgtcttttt cgtatatttc tcttctcttc ttactcattg gtagagattg ctcattctct
    16381 tctaatacta ttttttctcc tgtaactttt tctatagctt ctatgttatt taatttaaca
    16441 tttggattcc attttctttc aggagcttta acttctacac cttctatagc atttttaagc
    16501 atagccatta ttctaacagc atcttctgga gctaaagtgt tgttagataa tatttcattc
    16561 tctatacctt gcttagactt attgttgtct accatgtgag tcatgtattt attaccaaca
    16621 actagagcac cttgaacagc tgagaaactt acaccaacta ctggtatacc tctcatacct
    16681 atttgctcta tgtgagatgc gaaatctata tggttgtttc cgaaaccttc agtagttact
    16741 acagctccat caacttccat agcttcaact aacataccta atctcttaga tacatagaat
    16801 ttctcagcat ttacttgtgg agaaccaaca aatacaacac ctactaaatc tatttcttcg
    16861 tcttccatag ctcttattac taatggctct ctccagtaat gtcttgacat ttcttttgat
    16921 gcaggtccta tacaagttaa tgcatggata ccaccatcta atacttcaag tggagataat
    16981 acaactggca tgtttcctaa gtcaacgttt ggttgagctc ctaatgtacc aactggctca
    17041 actggtaata ttaggttatc atgcattgca ccttgtccca ttatttcttt tataacaaca
    17101 actttctttt taccttctct tctgtattgc tctattactt cttcatcaac tactaaagag
    17161 ttgtcaactt tctttaatgc ttctcttatt tcttgagtta catagtcaaa tgcacggtga
    17221 gcagctaaag gtcctggtct ttccatgtta gttcctgctt ttattgttac ttgacctttg
    17281 atgaatattt cacctttgtc agcagcacct ggtctacccc acattatagt agtatttaac
    17341 tcaccttctg aagaaccaaa ttcacctatt tgaactccat cttcatctgt accagttaat
    17401 accattacag ctccatctat aactctagtt atacctgaac ctaattcgcc ttcttcttta
    17461 gtagcgattg gttgtatatc taatacagct tcactgtaag tttcatattt atcaggagtt
    17521 attatttcta acttcatatc tacaactaac tcttgagttt cattagcttc tttacatatt
    17581 tcttctgcgt ttctgatgta taaagtagtt ccttctattt tagtttcatc agcaaattca
    17641 actttatcta ttttaaagtg ttttttagtt aagcttctta aaagtttctt ttcttcaact
    17701 tttggagcta cttctgctgc tgcacttgcc actggagcag caacgtttgc tactggagca
    17761 gcaactcctg cttgaccagc tactgataaa ggtatctcta agcttatgtt cttaccttca
    17821 cctatgtgta ttttaactgt ttcacctgca actcctgtgc ttactggaac tactgctgct
    17881 tctgatactg gagctgcttc ttctactgtt tcttctacta cttcttcttt agcctctcct
    17941 gctttgtatc cttctacgtt atctgtagtc attggagaaa gtgcatctaa agtttctttt
    18001 aacttagctc ctagtacttg acctatagtt aatccatttt ctggtattgt taataatcca
    18061 gaatcctcta agtctgcaaa tattgctgga tcttctaagt tttctggcgc tataattgtt
    18121 cccgcttcaa atctacaaca acaaactgct gggtcatttg catgggcttg agctgtttct
    18181 aaagttattg acatagtata aaatacctcc ttacattatt ttcttattta ttattttaaa
    18241 tctcaattcc tcaacaggta accccttaac tgagaaatta agtaaattaa aacctacata
    18301 atttgttatg attaatatta tttttttctc acttaaagtc actttatatt ataagcaatt
    18361 cctatgccaa cttattaaca aaaagatttt ttctataata gattcttcat ctatcgctat
    18421 ttttagccaa atattatata aatatttatt taaaatatgt tattttttgt atttgtaaac
    18481 attttctcaa acaaaaaaag tgtcaattaa tcaacacttt ttcatttttt ttaacacatc
    18541 tatatacaca acacttatac aagtgttggc tttactaggt tttaaattaa agtttatcat
    18601 tttgtcaaaa tttagtcatg tgtcatattt tagacacatc tattttgtat tggtctatct
    18661 tataatacaa agttgttctg ggtatattta atatcttagc tgctttagct ttatttccat
    18721 tagacatttt aagtgccttg gttatattct ttatttcaat tttttgagtt gccttcgtta
    18781 aatctaaagg atactcttct tcttcagtac tattatttgt agaatccatc atataacttg
    18841 gtacatcatc cacatctatt ttagaatttt gtgacaatac aacaatattt tcaatcgtat
    18901 tttttaattc tctaatatta cctttccatc tataattctg aaatatatct attacatctt
    18961 tgctaattgt aagaacaggc ttattatttt gtttacatat ctcctctaaa aagctatgaa
    19021 ccaataatcc tatgtcttct tttctctctc taagtggagg aatctttatt tcaacaacat
    19081 ttaatctata gtataaatct tctctaaatt tttctgcctt taccatttta ctcaaatctt
    19141 tattggtagc tgatattatt ctaggattta tttttattgt ggtatctcca ccaactcttc
    19201 ttatttcttt ttcctgtaat actctaagca actttgcttg catactaaga ggtaaatcgg
    19261 caatttcatc taaaaataca gttccatctt ttgcaagttc aaatatacct attcgaccct
    19321 ttttacttgc tcctgtaaat gcacctgatt catacccaaa aaactcactt tcaaataact
    19381 catttggtat ggcactacag ttaacaggta taaattgccc ttttctctca ctataatcat
    19441 gaatagctct tgcaaatact tctttacctg taccactttc gccccaaata aatatactac
    19501 tatttgttct agccacttgt tttgcaatcg ctttcgattt ttccaattta taacttttac
    19561 ctaaaatctt atcaaaacta cctttagata aatttttgac ttcgttcttt agatatttta
    19621 atgtatcatt tactttttca agttcaagag ctaatatctt agcttctgta acatcaactt
    19681 ctgtacatac aacaccgata aattcttctt tataaaatat aggatttgca tgaactaatg
    19741 catacatatt ttctttttta tctgtaaaat acaagtcact cattccaact ttactattta
    19801 gtactttttc tgaaatagta ttttccaaaa attcattcat cggtcgacca actatatctt
    19861 cagactttat atcatacctt tcctccataa acttattcca caagataaca acaccttcgt
    19921 tatttatagc acaaattcct tctttaattt gacctataat atacttcaaa gttaatccat
    19981 aatcttctaa atgcatatat agatagtccc taatgtgctc ttgttttaga actccaataa
    20041 ttttattatc tctaagaaca ggaagaatcc ctatattttt taatatcatt atatctctac
    20101 attcatctaa ggttaaacct tcgttgacat atattacatc cttcttcatt ataagtttta
    20161 ctggttgacc ttcatacttt ttatgcatct cataaagatt atgtatatcc gtcatggata
    20221 taatgccttt taattggtca gatgaatcta ttaccattag agtttttgtg ttgcttttta
    20281 tcatttcttt tattgcatct tcaattcttg tgtcttcgtc tatagttgta aattttgtat
    20341 ccattacctc ttctactttt tttacttctg gtatcgaaaa cataaagtcc cccctttata
    20401 gtagtacctc atcaaaactt atttatatat atttattcta gcatattata ataattattt
    20461 attaaataat tattatta
//
LOCUS       DAMJVI010000068        20425 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.070, whole genome shotgun sequence.
ACCESSION   DAMJVI010000068 DAMJVI010000000
VERSION     DAMJVI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20425
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.070"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            113..667
                     /gene="tcdR"
                     /locus_tag="PC545_002480"
     CDS             113..667
                     /gene="tcdR"
                     /locus_tag="PC545_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HDF2457246.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLHYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKMKFNFVEFDE
                     NILNMKNNYQSKSIFEEDICFFGYILKELSGIQRKVIFYKYLKGYSDREISVKLKISR
                     QAVNKAKNRAFKKIKKDYENYFNL"
     gene            985..8085
                     /gene="tcdB"
                     /locus_tag="PC545_002481"
                     /gene_synonym="toxB"
     CDS             985..8085
                     /gene="tcdB"
                     /locus_tag="PC545_002481"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HDF2457247.1"
                     /translation="MSLVNRKQLEKMANVRFRTQEDEYVAILDALEEYHNMSENTVVE
                     KYLKLKDINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPVEKNLHFVW
                     IGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTVVESAINDTLESFREN
                     LNDPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKTYLSNEYSKEI
                     DELNTYIEESLNKITQNSGNDVRNFEEFKNGESFNLYEQELVERWNLAAASDILRISA
                     LKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEYIPEYTSEH
                     FDMLDEEVQSSFESVLASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIINQGLISVKD
                     SYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANADNGRFMMEL
                     GKYLRVGFFPDVKTTINLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRNFEISKTNI
                     SQSTEQEMASLWSFDDARAKAQFEEYKRNYFEGSLGEDDNLDFSQNIVVDKEYLLEKI
                     SSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSVLFQKNIEDSEIAYYYNPG
                     DGEIQEIDKYKIPSIISDRPKIKLTFIGHGKDEFNTDIFAGFDVDSLSTEIEAAIDLA
                     KEDISPKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQDSIIVSAN
                     QYEVRINSEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVKSKNLPELS
                     TLLQEIRNNSNSSDIELEEKVMLTECEINVISNIDTQIVEERIEEAKNLTSDSINYIK
                     DEFKLIESISDALCDLKQQNELEDSHFISFEDISETDEGFSIRFINKETGESIFVETE
                     KTIFSEYANHITEEISKIKGTIFDTVNGKLVKKVNLDTTHEVNTLNAAFFIQSLIEYN
                     SSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLLPTLSEGLP
                     IIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTTATTAIITSSLGVASG
                     FSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHVSLVETEGVFTLLDDKIMMP
                     QDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTDDIDHFFSAPSITYREPHLSIYD
                     VLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENDGTKLLDRIRDNYEGEF
                     YWRYFAFIADALITTLKPRYEDTNIRINLDSNTRSFIVPIITTEYIREKLSYSFYGSG
                     GTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIESDKIKKGDLIEGILSTLSIE
                     ENKIILNSHEINFSGEVNGSNGFVSLTFSILEGINAIIEVDLLSKSYKLLISGELKIL
                     MLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVSELPDVVLI
                     SKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSLTLQDEKTIKL
                     NSVHLDESGVAEILKFMNRKGNTNTSDSLMSFLESMNIKSIFVNFLQSNIKFILDANF
                     IISGTTSIGQFEFICDENDNIQPYFIKFNTLETNYTLYVGNRQNMIVEPNYDLDDSGD
                     ISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNTYPEVIVLDANYIN
                     EKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTLANKLSFNF
                     SDKQDVPVSEIILSFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSGLIYINDSL
                     YYFKPPVNNLITGFVTVGDDKYYFNPINGGAASIGETIIDDKNYYFNQSGVLQTGVFS
                     TEDGFKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFDDNYRGAVEWKELDGEMHYF
                     SPETGKAFKGLNQIGDYKYYFNSDGVMQKGFVSINDNKHYFDDSGVMKVGYTEIDGKH
                     FYFAENGEMQIGVFNTEDGFKYFAHHNEDLGNEEGEEISYSGILNFNNKIYYFDDSFT
                     AVVGWKDLEDGSKYYFDEDTAEAYIGLSLINDGQYYFNDDGIMQVGFVTINDKVFYFS
                     DSGIIESGVQNIDDNYFYIDDNGIVQIGVFDTSDGYKYFAPANTVNDNIYGQAVEYSG
                     LVRVGEDVYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDDIKYYFDEK
                     GIMRTGLISFENNNYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNTPDGFKYFA
                     HQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFDPDTAQLVI
                     SE"
     gene            8207..8707
                     /gene="tcdE"
                     /locus_tag="PC545_002482"
     CDS             8207..8707
                     /gene="tcdE"
                     /locus_tag="PC545_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HDF2457248.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAILLDAMTDEM
                     NAKDEK"
     gene            8786..8947
                     /locus_tag="PC545_002483"
     CDS             8786..8947
                     /locus_tag="PC545_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457249.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYIDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            9036..9218
                     /locus_tag="PC545_002484"
     CDS             9036..9218
                     /locus_tag="PC545_002484"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457250.1"
                     /translation="MRDEGQNDIENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            9435..17567
                     /gene="tcdA"
                     /locus_tag="PC545_002485"
                     /gene_synonym="toxA"
     CDS             9435..17567
                     /gene="tcdA"
                     /locus_tag="PC545_002485"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HDF2457251.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKTSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFINYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     VLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNGVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKNSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDASVSPDTKFILNNLKLNIESSIGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDVLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKKYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNSIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIETENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENDGTRLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNDSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQVKV
                     NGLYLNESVYSSYLDFVKNSDGHHNTSNFMNLFLDNISFWKLFGFENINFVIDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLGYIMSNFKSFNSENELDRDHLGFKIIDNKTYYYDEDSKLVKGLININNSLFY
                     FDPIEFNLVTGWQTINGKKYYFDINTGAALTSYKIINGKHFYFNNDGVMQLGVFKGPD
                     GFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKYYFDNDSKAVTGWRIINNEKYYFNP
                     NNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVNGSRYYFDTDTAIAFNGYKTIDGKH
                     FYFDSDCVVKIGVFSTSNGFEYFAPANTYNNNIEGQAIVYQSKFLTLNGKKYYFDNNS
                     KAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKKYYFNTNTAEAATGWQTIDGKKYYF
                     NTNTAIASTGYTIINGKHFYFNTDGIMQIGVFKGPNGFEYFAPANTDANNIEGQAILY
                     QNEFLTLNGKKYYFGSDSKAVTGWRIINNKKYYFNPNNAIAAIHLCTINNDKYYFSYD
                     GILQNGYITIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQN
                     KFLTLNGKKYYFDNDSKAVTGWQTIDGKKYYFNLNTAEAATGWQTIDGKKYYFNLNTA
                     EAATGWQTIDGKKYYFNTNTFIASTGYTSINGKHFYFNTDGIMQIGVFKGPNGFEYFA
                     PANTHNNNIEGQAILYQNKFLTLNGKKYYFGSDSKAVTGLRTIDGKKYYFNTNTAVAV
                     TGWQTINGKKYYFNTNTSIASTGYTIISGKHFYFNTDGIMQIGVFKGPDGFEYFAPAN
                     TDANNIEGQAIRYQNRFLYLHDNIYYFGNNSKAATGWVTIDGNRYYFEPNTAMGANGY
                     KTIDNKNFYFRNGLPQIGVFKGSNGFEYFAPANTDANNIEGQAIRYQNRFLHLLGKIY
                     YFGNNSKAVTGWQTINGKVYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
     gene            complement(17902..18600)
                     /gene="tcdC"
                     /locus_tag="PC545_002486"
     CDS             complement(17902..18600)
                     /gene="tcdC"
                     /locus_tag="PC545_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin anti-sigma factor TcdC"
                     /protein_id="HDF2457252.1"
                     /translation="MFSKKNDGNEFSNEGKGSSKKIIKFFKSTKGIAPLAFILGVFFG
                     NISSPACSEDHEEVISNQTSVIDSQKTEIETLNSKLSDAEPWFKMKDDEKKAIEAENQ
                     RKAEEAKKAEEAKKAEEQRKKEEEEKKGYDTGITYDQLARTPDDYKYKKVKFEGKVIQ
                     VIEDGDEVQIRLAVSGNYDKVVLCSYKKSITPSRVLEDDYITIRGISAGTITYESTMG
                     GNITIPGIAVEKIN"
     gene            19026..19271
                     /locus_tag="PC545_002487"
     CDS             19026..19271
                     /locus_tag="PC545_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HDF2457253.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLI
                     RGQHIDRCQLYVYRCAVYFASTENPEPEKLKWWYWKD"
     gene            complement(19489..19758)
                     /locus_tag="PC545_002488"
     CDS             complement(19489..19758)
                     /locus_tag="PC545_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2457254.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNKGKDQGFDPMVNIEQCRKWLEETVSN"
BASE COUNT         8093 a         2294 c         3088 g         6950 t
ORIGIN      
        1 atataaaatt taatttattt gccgattata taattataat gactgattta attccgatgt
       61 tgtcaaaatt ttcaaataaa tcatcattat aaatataaga gaggatgatt ttatgcaaaa
      121 gtctttttat gaattaattg ttttagcaag aaataactca gtagatgatt tgcaagaaat
      181 tttatttatg tttaagccat tagtaaaaaa gcttagtaga gttttacatt atgaagaggg
      241 agaaacagat ttaataatat tttttattga attaataaaa aatattaaat taagtagctt
      301 ttcagaaaaa agcgatgcta ttatagtcaa atatattcat aaatcattac tgaataagac
      361 ttttgagttg tctagaagat attctaaaat gaagtttaat tttgtagaat ttgatgaaaa
      421 tatcttaaat atgaaaaata attatcaaag taagtctatt tttgaggaag atatttgttt
      481 tttcggatat attttgaaag aattatctgg tattcaaaga aaagttattt tttataaata
      541 tttaaaagga tattctgata gagaaatatc agtgaaatta aaaatatcta gacaagctgt
      601 taataaggct aaaaatagag catttaaaaa aataaaaaaa gactatgaaa attattttaa
      661 cttgtaatta atgagcttaa agaaatattt acaatagaaa tcaaatttta gaattaactt
      721 tattgtaaaa tcaataactt aatctaagaa tatcttaatt tttatatttt atatagaaca
      781 aagtttacat atttatttca gacaacgtct ttattcaatc gaagagcaaa ttaatcaact
      841 gagtgtcttc aatttaaaat gttaggaagt gaatgtatat gaaaacctaa gtagatatta
      901 gtatatttta taaatagaaa ggaggatata taaaagagtt ttatcattta gatgtaaaaa
      961 tattatagta aaggagaaaa ttttatgagt ttagttaata gaaaacagtt agaaaaaatg
     1021 gcaaatgtaa gatttcgtac tcaagaagat gaatatgttg caatattgga tgctttagaa
     1081 gaatatcata atatgtcaga gaatactgta gtcgaaaaat atttaaaatt aaaagatata
     1141 aatagtttaa cagatattta tatagataca tataaaaaat ctggtagaaa taaagcctta
     1201 aaaaaattta aggaatatct agttacagaa gtattagagc taaagaataa taatttaact
     1261 ccagttgaga aaaatttaca ttttgtttgg attggaggtc aaataaatga cactgctatt
     1321 aattatataa atcaatggaa agatgtaaat agtgattata atgttaatgt tttttatgat
     1381 agtaatgcat ttttgataaa cacattgaaa aaaactgtag tagaatcagc aataaatgat
     1441 acacttgaat catttagaga aaacttaaat gaccctagat ttgactataa taaattcttc
     1501 agaaaacgta tggaaataat ttatgataaa cagaaaaatt tcataaacta ctataaagct
     1561 caaagagaag aaaatcctga acttataatt gatgatattg taaagacata tctttcaaat
     1621 gagtattcaa aggagataga tgaacttaat acctatattg aagaatcctt aaataaaatt
     1681 acacagaata gtggaaatga tgttagaaac tttgaagaat ttaaaaatgg agagtcattc
     1741 aacttatatg aacaagagtt ggtagaaagg tggaatttag ctgctgcttc tgacatatta
     1801 agaatatctg cattaaaaga aattggtggt atgtatttag atgttgatat gttaccagga
     1861 atacaaccag acttatttga gtctatagag aaacctagtt cagtaacagt ggatttttgg
     1921 gaaatgacaa agttagaagc tataatgaaa tacaaagaat atataccaga atatacctca
     1981 gaacattttg acatgttaga cgaagaagtt caaagtagtt ttgaatctgt tctagcttct
     2041 aagtcagata aatcagaaat attctcatca cttggtgata tggaggcatc accactagaa
     2101 gttaaaattg catttaatag taagggtatt ataaatcaag ggctaatttc tgtgaaagac
     2161 tcatattgta gcaatttaat agtaaaacaa atcgagaata gatataaaat attgaataat
     2221 agtttaaatc cagctattag cgaggataat gattttaata ctacaacgaa tacctttatt
     2281 gatagtataa tggctgaagc taatgcagat aatggtagat ttatgatgga actaggaaag
     2341 tatttaagag ttggtttctt cccagatgtt aaaactacta ttaacttaag tggccctgaa
     2401 gcatatgcgg cagcttatca agatttatta atgtttaaag aaggcagtat gaatatccat
     2461 ttgatagaag ctgatttaag aaactttgaa atctctaaaa ctaatatttc tcaatcaact
     2521 gaacaagaaa tggctagctt atggtcattt gacgatgcaa gagctaaagc tcaatttgaa
     2581 gaatataaaa ggaattattt tgaaggttct cttggtgaag atgataatct tgatttttct
     2641 caaaatatag tagttgacaa ggagtatctt ttagaaaaaa tatcttcatt agcaagaagt
     2701 tcagagagag gatatataca ctatattgtt cagttacaag gagataaaat tagttatgaa
     2761 gcagcatgta acttatttgc aaagactcct tatgatagtg tactatttca gaaaaatata
     2821 gaagattcag aaattgcata ttattataat cctggagatg gtgaaataca agaaatagac
     2881 aagtataaaa ttccaagtat aatttctgat agacctaaga ttaaattaac atttattggt
     2941 catggtaaag atgaatttaa tactgatata tttgcaggtt ttgatgtaga ttcattatcc
     3001 acagaaatag aagcagcaat agatttagct aaagaggata tttctcctaa gtcaatagaa
     3061 ataaatttat taggatgtaa tatgtttagc tactctatca acgtagagga gacttatcct
     3121 ggaaaattat tacttaaagt taaagataaa atatcagaat taatgccatc tataagtcaa
     3181 gactctatta tagtaagtgc aaatcaatat gaagttagaa taaatagtga aggaagaaga
     3241 gaattattgg atcattctgg tgaatggata aataaagaag aaagtattat aaaggatatt
     3301 tcatcaaaag aatatatatc atttaatcct aaagaaaata aaattacagt aaaatctaaa
     3361 aatttacctg agctatctac attattacaa gaaattagaa ataattctaa ttcaagtgat
     3421 attgaactag aagaaaaagt aatgttaaca gaatgtgaga taaatgttat ttcaaatata
     3481 gatacgcaaa ttgttgagga aaggattgaa gaagctaaga atttaacttc tgactctatt
     3541 aattatataa aagatgaatt taaactaata gaatctattt ctgatgcact atgtgactta
     3601 aaacaacaga atgaattaga agattctcat tttatatctt ttgaggacat atcagagact
     3661 gatgagggat ttagtataag atttattaat aaagaaactg gagaatctat atttgtagaa
     3721 actgaaaaaa caatattctc tgaatatgct aatcatataa ctgaagagat ttctaagata
     3781 aaaggtacta tatttgatac tgtaaatggt aagttagtaa aaaaagtaaa tttagatact
     3841 acacacgaag taaatacttt aaatgctgca ttttttatac aatcattaat agaatataat
     3901 agttctaaag aatctcttag taatttaagt gtagcaatga aagtccaagt ttacgctcaa
     3961 ttatttagta ctggtttaaa tactattaca gatgcagcca aagttgttga attagtatca
     4021 actgcattag atgaaactat agacttactt cctacattat ctgaaggatt acctataatt
     4081 gcaactatta tagatggtgt aagtttaggt gcagcaatca aagagctaag tgaaacgagt
     4141 gacccattat taagacaaga aatagaagct aagataggta taatggcagt aaatttaaca
     4201 acagctacaa ctgcaatcat tacttcatct ttgggggtag ctagtggatt tagtatactt
     4261 ttagttcctt tagcaggaat ttcagcaggt ataccaagct tagtaaacaa tgaacttgta
     4321 cttcgagata aggcaacaaa ggttgtagat tattttaaac atgtttcatt agttgaaact
     4381 gaaggagtat ttactttatt agatgataaa ataatgatgc cacaagatga tttagtgata
     4441 tcagaaatag attttaataa taattcaata gttttaggta aatgtgaaat ctggagaatg
     4501 gaaggtggtt caggtcatac tgtaactgat gatatagatc acttcttttc agcaccatca
     4561 ataacatata gagagccaca cttatctata tatgacgtat tggaagtaca aaaagaagaa
     4621 cttgatttgt caaaagattt aatggtatta cctaatgctc caaatagagt atttgcttgg
     4681 gaaacaggat ggacaccagg tttaagaagc ttagaaaatg atggcacaaa actgttagac
     4741 cgtataagag ataactatga aggtgagttt tattggagat attttgcttt tatagctgat
     4801 gctttaataa caacattaaa accaagatat gaagatacta atataagaat aaatttagat
     4861 agtaatacta gaagttttat agttccaata ataactacag aatatataag agaaaaatta
     4921 tcatattctt tctatggttc aggaggaact tatgcattgt ctctttctca atataatatg
     4981 ggtataaata tagaattaag tgaaagtgat gtttggatta tagatgttga taatgttgtg
     5041 agagatgtaa ctatagaatc tgataaaatt aaaaaaggtg atttaataga aggtatttta
     5101 tctacactaa gtattgaaga gaataaaatt atcttaaata gccatgagat taatttttct
     5161 ggtgaggtaa atggaagtaa tggatttgtt tctttaacat tttcaatttt agaaggaata
     5221 aatgcaatta tagaagttga tttattatct aaatcatata aattacttat ttctggcgaa
     5281 ttaaaaatat tgatgttaaa ttcaaatcat attcaacaga aaatagatta tataggattc
     5341 aatagcgaat tacagaaaaa tataccatat agctttgtag atagtgaagg aaaagagaat
     5401 ggttttatta atggttcaac aaaagaaggt ttatttgtat ctgaattacc tgatgtagtt
     5461 cttataagta aggtttatat ggatgatagt aagccttcat ttggatatta tagtaataat
     5521 ttgaaagatg tcaaagttat aactaaagat aatgttaata tattaacagg ttattatctt
     5581 aaggatgata taaaaatctc tctttctttg actctacaag atgaaaaaac tataaagtta
     5641 aatagtgtgc atttagatga aagtggagta gctgagattt tgaagttcat gaatagaaaa
     5701 ggtaatacaa atacttcaga ttctttaatg agctttttag aaagtatgaa tataaaaagt
     5761 attttcgtta atttcttaca atctaatatt aagtttatat tagatgctaa ttttataata
     5821 agtggtacta cttctattgg ccaatttgag tttatttgtg atgaaaatga taatatacaa
     5881 ccatatttca ttaagtttaa tacactagaa actaattata ctttatatgt aggaaataga
     5941 caaaatatga tagtggaacc aaattatgat ttagatgatt ctggagatat atcttcaact
     6001 gttatcaatt tctctcaaaa gtatctttat ggaatagaca gttgtgttaa taaagttgta
     6061 atttcaccaa atatttatac agatgaaata aatataacgc ctgtatatga aacaaataat
     6121 acttatccag aagttattgt attagatgca aattatataa atgaaaaaat aaatgttaat
     6181 atcaatgatc tatctatacg atatgtatgg agtaatgatg gtaatgattt tattcttatg
     6241 tcaactagtg aagaaaataa ggtgtcacaa gttaaaataa gattcgttaa tgtttttaaa
     6301 gataagactt tggcaaataa gctatctttt aactttagtg ataaacaaga tgtacctgta
     6361 agtgaaataa tcttatcatt tacaccttca tattatgagg atggattgat tggctatgat
     6421 ttgggtctag tttctttata taatgagaaa ttttatatta ataactttgg aatgatggta
     6481 tctggattaa tatatattaa tgattcatta tattatttta aaccaccagt aaataatttg
     6541 ataactggat ttgtgactgt aggcgatgat aaatactact ttaatccaat taatggtgga
     6601 gctgcttcaa ttggagagac aataattgat gacaaaaatt attatttcaa ccaaagtgga
     6661 gtgttacaaa caggtgtatt tagtacagaa gatggattta aatattttgc cccagctaat
     6721 acacttgatg aaaacctaga aggagaagca attgatttta ctggaaaatt aattattgac
     6781 gaaaatattt attattttga tgataattat agaggagctg tagaatggaa agaattagat
     6841 ggtgaaatgc actattttag cccagaaaca ggtaaagctt ttaaaggtct aaatcaaata
     6901 ggtgattata aatactattt caattctgat ggagttatgc aaaaaggatt tgttagtata
     6961 aatgataata aacactattt tgatgattct ggtgttatga aagtaggtta cactgaaata
     7021 gatggcaagc atttctactt tgctgaaaac ggagaaatgc aaataggagt atttaataca
     7081 gaagatggat ttaaatattt tgctcatcat aatgaagatt taggaaatga agaaggtgaa
     7141 gaaatctcat attctggtat attaaatttc aataataaaa tttactattt tgatgattca
     7201 tttacagctg tagttggatg gaaagattta gaggatggtt caaagtatta ttttgatgaa
     7261 gatacagcag aagcatatat aggtttgtca ttaataaatg atggtcaata ttattttaat
     7321 gatgatggaa ttatgcaagt tggatttgtc actataaatg ataaagtctt ctacttctct
     7381 gactctggaa ttatagaatc tggagtacaa aacatagatg acaattattt ctatatagat
     7441 gataatggta tagttcaaat tggtgtattt gatacttcag atggatataa atattttgca
     7501 cctgctaata ctgtaaatga taatatttac ggacaagcag ttgaatatag tggtttagtt
     7561 agagttgggg aagatgtata ttattttgga gaaacatata caattgagac tggatggata
     7621 tatgatatgg aaaatgaaag tgataaatat tatttcaatc cagaaactaa aaaagcatgc
     7681 aaaggtatta atttaattga tgatataaaa tattattttg atgagaaggg cataatgaga
     7741 acgggtctta tatcatttga aaataataat tattacttta atgagaatgg tgaaatgcaa
     7801 tttggttata taaatataga agataagatg ttctattttg gtgaagatgg tgtcatgcag
     7861 attggagtat ttaatacacc agatggattt aaatactttg cacatcaaaa tactttggat
     7921 gagaattttg agggagaatc aataaactat actggttggt tagatttaga tgaaaagaga
     7981 tattatttta cagatgaata tattgcagca actggttcag ttattattga tggtgaggag
     8041 tattattttg atcctgatac agctcaatta gtgattagtg aatagataaa aatatgttaa
     8101 atatatcctc ttatacttaa atatataaaa ataaacaaaa tgatacacta cataaagtgt
     8161 tctatctaat atgaagattt accaataaaa aggtggacta tgatgaatgc acagtagttc
     8221 acctttttat atttctaatg gtaacaaaat atttttttat ataaacctag gaggcgttat
     8281 gaatatgaca atatcttttt tatcagagca tatatttata aagttagtaa ttttaactat
     8341 atcatttgat acattattag gatgtttaag tgcaataaaa agtcgtaaat ttaattctag
     8401 ttttggaata gatggaggaa tcagaaaagt agcaatgata gcatgtatat tttttttatc
     8461 agtagttgac attcttacaa agtttaactt tttatttatg ttaccacaag attgtatcaa
     8521 ttttttaaga ctaaaacatc ttggaatatc tgaatttttc tctattttat ttattttata
     8581 tgaaagtgta agtatattaa aaaatatgtg cttatgtgga ttaccagtac ctaagagatt
     8641 aaaggaaaaa atagcaattt tactagatgc aatgacagat gaaatgaatg ctaaggatga
     8701 aaagtaagta atggtagata taataaagat attaacaaat aaaaagtgtt atccaaataa
     8761 gaatagctga aagttatcat aattcatgaa actaataatg aaaacgaggg agcagatgcc
     8821 aagagacaca caagtattaa atacatataa tttcgaagca agtgttcatt actatataga
     8881 tgacaaggta gtatatcaaa cattggttca caaagatggt gcatggtcag ttggtaaaat
     8941 ctattaagct acattagtta cagatatcac aaactataat agttaaacat agaaatatgt
     9001 gtaaattgtg atggaaatta ttcaaaaaca caaaaatacg tgatgaagga caaaatgata
     9061 tagaaaataa gtatcaaacc ttaataaatg atttaattga tagtttaaaa gttataggaa
     9121 aaatatataa agaaataaaa acattaaaaa aatataagat atgtttacaa attactatca
     9181 gacaatctcc ttatctaata gaagagtcaa ttaactaatt gagtatcttt aaattgaaat
     9241 gttaggaagt gatttaaata tgaaaactta aattataaaa aatcaatatt aatttatttt
     9301 taaaaaatag aaaggagtgt ataagattta ttttcaaagt ttaaaaacaa gaaaatcaat
     9361 ttaaatttca gaaggaataa atgtggttat agaagtggat ttattatcaa aaataataat
     9421 actaggaggt ttttatgtct ttaatatcta aagaagagtt aataaaactc gcatatagca
     9481 ttagaccaag agaaaatgag tataaaacta tactaactaa tttagacgaa tataataagt
     9541 taactacaaa caataatgaa aataaatatt tacaattaaa aaaactaaat gaatcaattg
     9601 atgtttttat gaataaatat aaaacttcaa gcagaaatag agcactctct aatctaaaaa
     9661 aagatatatt aaaagaagta attcttatta aaaattccaa tacaagccct gtagaaaaaa
     9721 atttacattt tgtatggata ggtggagaag tcagtgatat tgctcttgaa tacataaaac
     9781 aatgggctga tattaatgca gaatataata ttaaactgtg gtatgatagt gaagcattct
     9841 tagtaaatac actaaaaaag gctatagttg aatcttctac cactgaagca ttacagctac
     9901 tagaggaaga gattcaaaat cctcaatttg ataatatgaa attttacaaa aaaaggatgg
     9961 aatttatata tgatagacaa aaaaggttta taaattatta taaatctcaa atcaataaac
    10021 ctacagtacc tacaatagat gatattataa agtctcatct agtatctgaa tataatagag
    10081 atgaaactgt attagaatca tatagaacaa attctttgag aaaaataaat agtaatcatg
    10141 gtatagatat cagggctaat agtttgttta cagaacaaga gttattaaat atttatagtc
    10201 aggagttgtt aaatcgtgga aatttagctg cagcatctga catagtaaga ttattagccc
    10261 taaaaaattt tggcggagta tatttagatg ttgatatgct tccaggtatt cactctgatt
    10321 tatttaaaac aatatctaga cctagctcta ttggactaga ccgttgggaa atgataaaat
    10381 tagaggctat tatgaagtat aaaaaatata taaataatta tacatcagaa aactttgata
    10441 aacttgatca acaattaaaa gataatttta aactcattat agaaagtaaa agtgaaaaat
    10501 ctgagatatt ttctaaatta gaaaatttaa atgtatctga tcttgaaatt aaaatagctt
    10561 tcgctttagg cagtgttata aatcaagcct tgatatcaaa acaaggttca tatcttacta
    10621 acctagtaat agaacaagta aaaaatagat atcaattttt aaaccaacac cttaacccag
    10681 ccatagagtc tgataataac ttcacagata ctactaaaat ttttcatgat tcattattta
    10741 attcagctac cgcagaaaac tctatgtttt taacaaaaat agcaccatac ttacaagtag
    10801 gttttatgcc agaagctcgc tccacaataa gtttaagtgg tccaggagct tatgcgtcag
    10861 cttactatga tttcataaat ttacaagaaa atactataga aaaaacttta aaagcatcag
    10921 atttaataga atttaaattc ccagaaaata atctatctca attgacagaa caagaaataa
    10981 atagtctatg gagctttgat caagcaagtg caaaatatca atttgagaaa tatgtaagag
    11041 attatactgg tggatctctt tctgaagaca atggggtaga ctttaataaa aatactgccc
    11101 tcgacaaaaa ctatttatta aataataaaa ttccatcaaa caatgtagaa gaagctggaa
    11161 gtaaaaatta tgttcattat atcatacagt tacaaggaga tgatataagt tatgaagcaa
    11221 catgcaattt attttctaaa aatcctaaaa atagtattat tatacaacga aatatgaatg
    11281 aaagtgcaaa aagctacttt ttaagtgatg atggagaatc tattttagaa ttaaataaat
    11341 ataggatacc tgaaagatta aaaaataagg aaaaagtaaa agtaaccttt attggacatg
    11401 gtaaagatga attcaacaca agcgaatttg ctagattaag tgtagattca ctttccaatg
    11461 agataagttc atttttagat accataaaat tagatatatc acctaaaaat gtagaagtaa
    11521 acttacttgg atgtaatatg tttagttatg attttaatgt tgaagaaact tatcctggga
    11581 agttgctatt aagtattatg gacaaaatta cttccacttt acctgatgta aataaaaatt
    11641 ctattactat aggagcaaat caatatgaag taagaattaa tagtgaggga agaaaagaac
    11701 ttctggctca ctcaggtaaa tggataaata aagaagaagc tattatgagc gatttatcta
    11761 gtaaagaata catttttttt gattctatag ataataagct aaaagcaaag tccaagaata
    11821 ttccaggatt agcatcaata tcagaagata taaaaacatt attacttgat gcaagtgtta
    11881 gtcctgatac aaaatttatt ttaaataatc ttaagcttaa tattgaatct tctattggtg
    11941 attacattta ttatgaaaaa ttagagcctg ttaaaaatat aattcacaat tctatagatg
    12001 atttaataga tgagttcaat ctacttgaaa atgtatctga tgaattatat gaattaaaaa
    12061 aattaaataa tctagatgag aagtatttaa tatcttttga agatatctca aaaaataatt
    12121 caacttactc tgtaagattt attaacaaaa gtaatggtga gtcagtttat gtagaaacag
    12181 aaaaagaaat tttttcaaaa tatagcgaac atattacaaa agaaataagt actataaaga
    12241 atagtataat tacagatgtt aatggtaatt tattggataa tatacagtta gatcatactt
    12301 ctcaagttaa tacattaaac gcagcattct ttattcaatc attaatagat tatagtagca
    12361 ataaagatgt actgaatgat ttaagtacct cagttaaggt tcaactttat gctcaactat
    12421 ttagtacagg tttaaatact atatatgact ctatccaatt agtaaattta atatcaaatg
    12481 cagtaaatga tactataaat gtactaccta caataacaga ggggatacct attgtatcta
    12541 ctatattaga cggaataaac ttaggtgcag caattaagga attactagac gaacatgacc
    12601 cattactaaa aaaagaatta gaagctaagg tgggtgtttt agcaataaat atgtcattat
    12661 ctatagctgc aactgtagct tcaattgttg gaataggtgc tgaagttact attttcttat
    12721 tacctatagc tggtatatct gcaggaatac cttcattagt taataatgaa ttaatattgc
    12781 atgataaggc aacttcagtg gtaaactatt ttaatcattt gtctgaatct aaaaaatatg
    12841 gccctcttaa aacagaagat gataaaattt tagttcctat tgatgattta gtaatatcag
    12901 aaatagattt taataataat tcgataaaac taggaacatg taatatatta gcaatggagg
    12961 ggggatcagg acacacagtg actggtaata tagatcactt tttctcatct ccatctataa
    13021 gttctcatat tccttcatta tcaatttatt ctgcaatagg tatagaaaca gaaaatctag
    13081 atttttcaaa aaaaataatg atgttaccta atgctccttc aagagtgttt tggtgggaaa
    13141 ctggagcagt tccaggttta agatcattgg aaaatgacgg aactagatta cttgattcaa
    13201 taagagattt atacccaggt aaattttact ggagattcta tgcttttttc gattatgcaa
    13261 taactacatt aaaaccagtt tatgaagaca ctaatattaa aattaaacta gataaagata
    13321 ctagaaactt cataatgcca actataacta ctaacgaaat tagaaacaaa ttatcttatt
    13381 catttgatgg agcaggagga acttactctt tattattatc ttcatatcca atatcaacga
    13441 atataaattt atctaaagat gatttatgga tatttaatat tgataatgaa gtaagagaaa
    13501 tatctataga aaatggtact attaaaaaag gaaagttaat aaaagatgtt ttaagtaaaa
    13561 ttgatataaa taaaaataaa cttattatag gcaatcaaac aatagatttt tcaggcgata
    13621 tagataataa agatagatat atattcttga cttgtgagtt agatgataaa attagtttaa
    13681 taatagaaat aaatcttgtt gcaaaatctt atagtttgtt attgtctggg gataaaaatt
    13741 atttgatatc caatttatct aatactattg agaaaatcaa tactttaggc ctagatagta
    13801 aaaatatagc gtacaattac actgatgaat ctaataataa atattttgga gctatatcta
    13861 aaacaagtca aaaaagcata atacattata aaaaagacag taaaaatata ttagaatttt
    13921 ataatgacag tacattagaa tttaacagta aagattttat tgctgaagat ataaatgtat
    13981 ttatgaaaga tgatattaat actataacag gaaaatacta tgttgataat aatactgata
    14041 aaagtataga tttctctatt tctttagtta gtaaaaatca agtaaaagta aatggattat
    14101 atttaaatga atccgtatac tcatcttacc ttgattttgt gaaaaattca gatggacacc
    14161 ataatacttc taattttatg aatttatttt tggacaatat aagtttctgg aaattgtttg
    14221 ggtttgaaaa tataaatttt gtaatcgata aatactttac ccttgttggt aaaactaatc
    14281 ttggatatgt agaatttatt tgtgacaata ataaaaatat agatatatat tttggtgaat
    14341 ggaaaacatc gtcatctaaa agcactatat ttagcggaaa tggtagaaat gttgtagtag
    14401 agcctatata taatcctgat acgggtgaag atatatctac ttcactagat ttttcctatg
    14461 aacctctcta tggaatagat agatatatca ataaagtatt gatagcacct gatttatata
    14521 caagtttaat aaatattaat accaattatt attcaaatga gtactaccct gagattatag
    14581 ttcttaaccc aaatacattc cacaaaaaag taaatataaa tttagatagt tcttcttttg
    14641 agtataaatg gtctacagaa ggaagtgact ttattttagt tagatactta gaagaaagta
    14701 ataaaaaaat attacaaaaa ataagaatca aaggtatctt atctaatact caatcattta
    14761 ataaaatgag tatagatttt aaagatatta aaaaactatc attaggatat ataatgagta
    14821 attttaaatc atttaattct gaaaatgaat tagatagaga tcatttagga tttaaaataa
    14881 tagataataa aacttattac tatgatgaag atagtaaatt agttaaagga ttaatcaata
    14941 taaataattc attattctat tttgatccta tagaatttaa cttagtaact ggatggcaaa
    15001 ctatcaatgg taaaaaatat tattttgata taaatactgg agcagcttta actagttata
    15061 aaattattaa tggtaaacac ttttatttta ataatgatgg tgtgatgcag ttgggagtat
    15121 ttaaaggacc tgatggattt gaatattttg cacctgccaa tactcaaaat aataacatag
    15181 aaggtcaggc tatagtttat caaagtaaat tcttaacttt gaatggcaaa aaatattatt
    15241 ttgataatga ctcaaaagca gtcactggat ggagaattat taacaatgag aaatattact
    15301 ttaatcctaa taatgctatt gctgcagtcg gattgcaagt aattgacaat aataagtatt
    15361 atttcaatcc tgacactgct atcatctcaa aaggttggca gactgttaat ggtagtagat
    15421 actactttga tactgatacc gctattgcct ttaatggtta taaaactatt gatggtaaac
    15481 acttttattt tgatagtgat tgtgtagtga aaataggtgt gtttagtacc tctaatggat
    15541 ttgaatattt tgcacctgct aatacttata ataataacat agaaggtcag gctatagttt
    15601 atcaaagtaa attcttaact ttgaatggta aaaaatatta ctttgataat aactcaaaag
    15661 cagttaccgg atggcaaact attgatagta aaaaatatta ctttaatact aacactgctg
    15721 aagcagctac tggatggcaa actattgatg gtaaaaaata ttactttaat actaacactg
    15781 ctgaagcagc tactggatgg caaactattg atggtaaaaa atattacttt aatactaaca
    15841 ctgctatagc ttcaactggt tatacaatta ttaatggtaa acatttttat tttaatactg
    15901 atggtattat gcagatagga gtgtttaaag gacctaatgg atttgaatat tttgcacctg
    15961 ctaatacgga tgctaacaac atagaaggtc aagctatact ttaccaaaat gaattcttaa
    16021 ctttgaatgg taaaaaatat tactttggta gtgactcaaa agcagttact ggatggagaa
    16081 ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgca attcatctat
    16141 gcactataaa taatgacaag tattacttta gttatgatgg aattcttcaa aatggatata
    16201 ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa atggtaacag
    16261 gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact cacaataata
    16321 acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat ggcaaaaaat
    16381 attattttga taatgactca aaagcagtta ctggatggca aaccattgat ggtaaaaaat
    16441 attactttaa tcttaacact gctgaagcag ctactggatg gcaaactatt gatggtaaaa
    16501 aatattactt taatcttaac actgctgaag cagctactgg atggcaaact attgatggta
    16561 aaaaatatta ctttaatact aacactttca tagcctcaac tggttataca agtattaatg
    16621 gtaaacattt ttattttaat actgatggta ttatgcagat aggagtgttt aaaggaccta
    16681 atggatttga atactttgca cctgctaata ctcataataa taacatagaa ggtcaagcta
    16741 tactttacca aaataaattc ttaactttga atggtaaaaa atattacttt ggtagtgact
    16801 caaaagcagt taccggattg cgaactattg atggtaaaaa atattacttt aatactaaca
    16861 ctgctgttgc agttactgga tggcaaacta ttaatggtaa aaaatactac tttaatacta
    16921 acacttctat agcttcaact ggttatacaa ttattagtgg taaacatttt tattttaata
    16981 ctgatggtat tatgcagata ggagtgttta aaggacctga tggatttgaa tactttgcac
    17041 ctgctaatac agatgctaac aatatagaag gtcaagctat acgttatcaa aatagattcc
    17101 tatatttaca tgacaatata tattattttg gtaataattc aaaagcggct actggttggg
    17161 taactattga tggtaataga tattacttcg agcctaatac agctatgggt gcgaatggtt
    17221 ataaaactat tgataataaa aatttttact ttagaaatgg tttacctcag ataggggtgt
    17281 ttaaagggtc taatggattt gaatactttg cacctgctaa tacggatgct aacaatatag
    17341 aaggtcaagc tatacgttat caaaatagat tcctacattt acttggaaaa atatattact
    17401 ttggtaataa ttcaaaagca gttactggat ggcaaactat taatggtaaa gtatattact
    17461 ttatgcctga tactgctatg gctgcagctg gtggactttt tgagattgat ggtgttatat
    17521 atttctttgg tgttgatgga gtaaaagccc ctgggatata tggctaaaat atatgtttga
    17581 taaaaaatta ttcctgtgct actaagaaat tatttttata taataaatat tgagatttaa
    17641 ttaaaagtca tgtgttattg taatacatga cttttagtta aaatttttct atcatttaat
    17701 aatctattat tcttgactat tttataataa aattcatata tggaaatatt aatactaaat
    17761 aattaatagt tgataaaaaa tagataatat gctaaaagca aaaactaatt tagagccttg
    17821 taactgttta tttgcaatta taaaaacatc tttaaacata ttgactataa tataaaatat
    17881 taactataat acaaaacaat attaattaat tttctctaca gctatccctg gtatagttat
    17941 atttccaccc atagttgatt cataagttat agttccagca cttatacctc ttatagttat
    18001 gtaatcatcc tctaatactc ttgaaggagt tattgatttt ttataactac atagtacaac
    18061 cttatcataa tttccagaca cagctaatct tatttgcacc tcatcaccat cttcaataac
    18121 ttgaataacc ttaccttcaa attttacctt tttgtactta taatcatcag gtgttctagc
    18181 taattggtca taagtaatac cagtatcata tcctttcttc tcctcttctt cttttttgcg
    18241 ttgttcttca gcctttttag cttcttcagc ttttttagct tcttcagctt tacgttgatt
    18301 ttcagcttca atagctttct tttcgtcgtc tttcattttg aaccatggtt cagcatcaga
    18361 caatttgcta tttaaagttt ctatttctgt tttttgagaa tctataactg atgtttggtt
    18421 agaaatgacc tcctcatggt cttcagaaca agctggtgag gatatattgc caaaaaacac
    18481 gcctaaaata aatgccagtg gagcaatacc ctttgtgctc ttaaagaatt taattatttt
    18541 cttagagctt ccttttcctt cattactaaa ttcgttacca tcattttttt tagaaaacat
    18601 aatacaatct cccttttatt agatatattt tattttaaaa gttaaaaact taaaataact
    18661 agttgactaa aaattatata ttcatatttt cctaaaatat atttccccta agcataattt
    18721 tacaaaataa agtaagaaga ttcaatatat tttgaaaaaa taagataaaa atgtcagatt
    18781 ataatataat tttcttgaag gtgaatttta tatattaaat taaacataaa gtattttatt
    18841 tgtataaaac tatattatga tgtaattaag tgactcagtg atttagaggt ggtaaaatat
    18901 atatatatga aagagcataa ataaattgat ataataaaaa attaaatatt aaatattaac
    18961 tataataaaa aaatatctgg taataggtat aatataaaaa gaatatacca gaaagaaggt
    19021 gaagtatggg agaactacaa aaaattccag gggtagggaa agcaacagaa aaatctttaa
    19081 tcatgttagg atatacaact attaaatcac ttaaagatgc aaacccagca caaatgtatg
    19141 aaaaggaatg tttaatacgt ggacagcaca tagacagatg tcagttatat gtatacagat
    19201 gtgcagtata ttttgcatca actgaaaatc cagaaccaga aaaattaaag tggtggtatt
    19261 ggaaagatta aaaaatcaaa aaagttagac ataaaaaaga gttgaatatc cgttttaaat
    19321 aataaaaact aatactcaac tcttttttag tcgtaaattt tatgtaaatc tatttgtatg
    19381 gctaaatata cccatcaata aatgtataaa tataccaatc aacaaatgac taaacatact
    19441 acctaacaaa ggattaaata aattacttaa caaagcattc taaaacaatt aattagaaac
    19501 tgtttcttca agccattttc tacattgctc aatatttacc atagggtcaa aaccttggtc
    19561 ttttccctta tttaaaagca tatcacaagg gaaagtatca cattcaccac aatgattaag
    19621 ttccttactt tcacagcaag ttttaacctt acattctcca ccccaaaatg gtttttccat
    19681 agttgggcaa ccagtgcaat ttacttgttc tttttttgta caactgtcac aacagacacc
    19741 acatcttgat tcaaacatgt tcaaacatcc tttccattat ttaaaaatat tataaaatga
    19801 ttttagatga atacaaaagt gcaaatagtt gattttaggt catctaaatg ttaaacataa
    19861 aattattagt actggtaaat ttataaaaac tattccaaaa atctttttga atatttcaaa
    19921 acacaataaa ttacttcaaa tccctttgaa tatattttac agcattatag attgctttaa
    19981 atctctttga atattctaca agactataga ttagtttaaa tccctttgaa tatattttac
    20041 aacattatag attgttttaa atctctttga atattctaca agactataga ttagtttaaa
    20101 tccctttgaa tatttcaaaa caccatagat tactttaaat ccctttgaat attctataac
    20161 actataaatt acttttaact tttatataac cataaagttt ttatctggca ctttaaatgg
    20221 agttttatat caaatttgcc ttccatcgaa taaccaaact taatttaatt acacattcca
    20281 agagtcaaat attcattcaa tatatttgat aattctatgt tatagctata attaaatata
    20341 gattattaaa gttaaatatt tatttaatat attgaatgat tttttaccta tatttaatta
    20401 agtttcatta tataatataa aaatt
//
LOCUS       DAMJVI010000069        20167 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.071, whole genome shotgun sequence.
ACCESSION   DAMJVI010000069 DAMJVI010000000
VERSION     DAMJVI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20167
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.071"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..601
                     /locus_tag="PC545_002489"
     CDS             <1..601
                     /locus_tag="PC545_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2457255.1"
                     /translation="VFENTNNHIGLDLGIKEFCISSCGDFIENPKYLKKSLNKLAKLQ
                     RELSRKTIGSLNRNKARLKVAGLQEHIANQRNDFLQKLSTKLIKENDIICIEDLQVKN
                     MIRNRKLSRLISDVSWSEFIRQLEYKANWYGRKIVKVGKFFASSQICNKCGYKNEEVK
                     DLNIREWICPSCNETHDRDINASINILKEGLRLITIQNK"
     gene            719..1024
                     /locus_tag="PC545_002490"
                     /pseudo
     CDS             719..1024
                     /locus_tag="PC545_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
     gene            1037..1942
                     /gene="truB"
                     /locus_tag="PC545_002491"
     CDS             1037..1942
                     /gene="truB"
                     /locus_tag="PC545_002491"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDF2457256.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEDIERA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKDIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNPNAIKYYSNGGSIDDKRFLKNNFDKDDEFVRVYST
                     DNFLGLGKLSKHNNTISVKSDKMFI"
     gene            2073..3002
                     /locus_tag="PC545_002492"
     CDS             2073..3002
                     /locus_tag="PC545_002492"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDF2457257.1"
                     /translation="MVIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMANVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            3106..3363
                     /gene="rpsO"
                     /locus_tag="PC545_002493"
     CDS             3106..3363
                     /gene="rpsO"
                     /locus_tag="PC545_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDF2457258.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            3603..4448
                     /locus_tag="PC545_002494"
     CDS             3603..4448
                     /locus_tag="PC545_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDF2457259.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFITEGYHIICMTGAANASGTFQSAMIA
                     KNDVNENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEEIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIVTIGCGDNLHDCEILKDEVNKRIKARRVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            4573..6684
                     /gene="pnp"
                     /locus_tag="PC545_002495"
     CDS             4573..6684
                     /gene="pnp"
                     /locus_tag="PC545_002495"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDF2457260.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCVGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            6950..7693
                     /locus_tag="PC545_002496"
     CDS             6950..7693
                     /locus_tag="PC545_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2457261.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDKSGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            7851..9098
                     /locus_tag="PC545_002497"
     CDS             7851..9098
                     /locus_tag="PC545_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDF2457262.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGI
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQINAFTSKECTCYYVKLIDEHIDTGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLIKKEIENIRENYLTEKEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVNINSIKKVVDK
                     VFNIENIGTCIVGRDVEKILHLD"
     gene            9133..9378
                     /locus_tag="PC545_002498"
     CDS             9133..9378
                     /locus_tag="PC545_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HDF2457263.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGDILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            9388..10611
                     /gene="dapG"
                     /locus_tag="PC545_002499"
     CDS             9388..10611
                     /gene="dapG"
                     /locus_tag="PC545_002499"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2457264.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKVKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKLITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            10724..11458
                     /locus_tag="PC545_002500"
     CDS             10724..11458
                     /locus_tag="PC545_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDF2457265.1"
                     /translation="MKDYREDNEKFDKDNCSEESSCEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            11581..13992
                     /locus_tag="PC545_002501"
     CDS             11581..13992
                     /locus_tag="PC545_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDF2457266.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIAGGWLISIF
                     ALILSVMFIFNISIKDLVSNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGLNNDDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGT
                     QSMPELDVLKELQKATNENPVVDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSN
                     YKKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEI
                     QPSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLE
                     SEEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYK
                     ANPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFAD
                     AQVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIV
                     ATQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAA
                     KPVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEA
                     IEFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQ
                     NLEGE"
     gene            13995..15053
                     /gene="mnmH"
                     /locus_tag="PC545_002502"
     CDS             13995..15053
                     /gene="mnmH"
                     /locus_tag="PC545_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDF2457267.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTVPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNILILKECINKF
                     RKRLGNKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYSKIINFNDTQKA
                     LEDVIDLYESILEGESEC"
     gene            15047..16381
                     /gene="rimO"
                     /locus_tag="PC545_002503"
     CDS             15047..16381
                     /gene="rimO"
                     /locus_tag="PC545_002503"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDF2457268.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILKELSEMHQIVSLNDIEFVFNEDLPRYISTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEEVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEVGEFVSVQINDAMEYDLMGDVLYELA"
     gene            16368..16910
                     /gene="pgsA"
                     /locus_tag="PC545_002504"
     CDS             16368..16910
                     /gene="pgsA"
                     /locus_tag="PC545_002504"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDF2457269.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNVPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVFLLLGAQFENVLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
     gene            17198..18244
                     /gene="recA"
                     /locus_tag="PC545_002505"
     CDS             17198..18244
                     /gene="recA"
                     /locus_tag="PC545_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDF2457270.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTTEIDEKVRAFYNLNEEH
                     EESGISVSKEIVEE"
     gene            18420..19961
                     /gene="rny"
                     /locus_tag="PC545_002506"
     CDS             18420..19961
                     /gene="rny"
                     /locus_tag="PC545_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDF2457271.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
BASE COUNT         8254 a         2044 c         3900 g         5969 t
ORIGIN      
        1 agtatttgaa aatactaata atcatatagg tttagattta ggaattaaag aattttgtat
       61 ctcaagttgt ggagacttta tagaaaatcc taagtatctt aagaagtcat taaacaagct
      121 tgctaaatta caaagagaat tatcgagaaa aacaatcggt agtttaaata gaaataaagc
      181 aagattaaaa gttgcaggac ttcaagaaca tatagctaat caaagaaatg attttctaca
      241 aaaactatct actaaattaa taaaagagaa tgatattatt tgcatagaag atttacaagt
      301 aaagaatatg attagaaatc gtaaactatc tcgcttaatt tcagatgtat catggtcaga
      361 atttattcgt caattagaat acaaggctaa ttggtatggt agaaaaattg taaaggtagg
      421 taaattcttt gcaagttcac aaatatgcaa taaatgtggg tataaaaacg aggaagttaa
      481 ggatttaaat ataagagaat ggatttgtcc tagttgtaat gaaactcacg atagagatat
      541 aaatgcaagt ataaatatat taaaagaagg attaagacta ataacgattc aaaataaata
      601 acttatgtaa gaaccgtagg aactacgggg atagcctgtg gagaattagt aagacatatt
      661 ttaatatgca aaattctgtg aagcaggaac cctgtgactt tagtcatggg aggttcagtc
      721 aaaggtatct agtatagttt taactcaaga catgatgaat agaaataata ttagctttaa
      781 tgatgttgat ggaataacaa catatacaag agacataaaa ggtatagaag ttggaatttt
      841 atttaaagag aaaaaacaaa atgaaattaa agtgagtttt aggtcaaaaa attatgttga
      901 tgtaagtgaa atagccaagt tatttggtgg tggtggtcat gtaagggctg ctggatgtac
      961 tattagagat agtattgata atgcaaagaa aatggtacta gaggcagtgt taaaatcaat
     1021 ttaaggtgac agtaaaatga ataagattat aagtatattg aaacccacag gtatgacatc
     1081 tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagttg gtcatacagg
     1141 tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag ctacaaaagt
     1201 atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttactt taggtataag
     1261 tacagatact tatgatgctt caggtgaaat tttaaaaaaa gttgatgatt ttaaatttag
     1321 taatgaagac atagaaagag cttttgacac acaaagagga gagataaatc aacttcctcc
     1381 aatttattca gctttaaagg taaatggaaa gagaatgtgt gatttagtaa gaagtggtag
     1441 acaatctgaa attactttaa aaacaagaag agtaaatata aaagacataa aaatactaag
     1501 tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt atgtgagaag
     1561 catttgccat gatataggag aatatctagg ttgtggagct catatgtcgt ttttgaatag
     1621 aacatcttct ggtaaatttg acttagataa ttcaataaca ttagaagaat tagaattatt
     1681 ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtcc ttgattcgtt
     1741 taattatgtt gtattaaacc ctaatgctat aaaatattat agtaatggtg gctcaataga
     1801 tgataaaagg tttttaaaaa ataatttcga taaagatgat gaatttgtaa gagtatacag
     1861 tacagataat ttcttaggat taggaaaatt atcaaaacac aataatacaa taagtgttaa
     1921 aagtgataag atgttcatat aaccaatgta tttaaaaaaa caacaaaaga gatagtacta
     1981 taataacttc tatagtctat ctcttttact tatgctataa tagtttgtga ataagttaag
     2041 agtaaattat attttagcgg ggaacattta ttatggtgat tataaaatct ataaaagaaa
     2101 tagcaaacat aaaagaaagt gtcattacca taggaaattt tgatggtgta cacaaaggac
     2161 atcaagttct tataggaaaa acagttgaac atgcaaaaaa agaaaatata cagagtatcg
     2221 tatttacatt tgcaaatcat cctgtaaatt actttagacc aaactcaaca aaaaatatta
     2281 taagcaatga tgataaaata aaattattag aagatttggg aatagatata gttgtaatca
     2341 taccatttga tgagtatatg actaaaatac ctgcaaagga tttcgtagat gagatactag
     2401 taaaaaaatt aaaagctaaa aagatagtaa ttggacatga ctttacattt gcaagggcaa
     2461 aagaaggcaa tgttaatcta ttaaaaagct tagaacatga atttggtttt gaggtagaag
     2521 taataaaacc catcaaaatc aatgatgtaa gagtgagtag tacttatatt agaagtttag
     2581 tatcccaggg tgatatggct aatgtcaagg aatatttggg tagaaattac aagttagaag
     2641 gttgtgttat acattctaaa caccttggaa gaactatagg attcccaacg gcaaatattg
     2701 accttaagaa taatatgctc gttccaaaga gaggtattta tgcttctatt gtacatattg
     2761 gagatgagac ttattttggt gctacaaatg taggctataa cccaacagtt aatggaaaaa
     2821 ctttgtctat tgaaacaaat attttagagt ttgatagaga tatttatggg gaaaatataa
     2881 ttgttgaatt tttggaaaga ataagagatg aaagaaagtt taattcaata gatgacttaa
     2941 aaaatcaatt gtataatgat acaaattatg tgtatgaaaa ctatgtttgc aaaaacaagt
     3001 aatgtatgat aaaatgatta aggaaaataa ccattactca gcatttgaat actccaatca
     3061 atgcttagta ataggcgttt ctaaaaaaat acggaggtaa tctaaatgat aaacaaatca
     3121 gaaataataa aagaatatgg aagagctgaa ggggatactg gttctccaga agtacaaata
     3181 gctttattaa cagctagaat aaacgagtta aatggacatt taaaaactca taagaaagac
     3241 caccattcaa gaagaggact attaaaaatg gttggtagaa gaagaaactt attagcttac
     3301 ttaaaggaaa aagatcttga aggatataga gcattaatag ctaaattagg attaagaaag
     3361 taatttcatt ctttataaaa aagcaggtat agtgtatacc tgctttttta tagttaattc
     3421 attataaagt aaaatagtta ataataaaag tagtttatat gttataaaaa attataaaat
     3481 gaataatatt gggtaagaat attaaaatag tatacaaata cataatcaat ttaaaaggaa
     3541 agacaggata aatatagaaa ttttataaca ggataattag ttggaataag gaggatatta
     3601 ttttgaaaat aaagttagta tgcgatagtt tgtgcgatat tccagatgaa atatcagaaa
     3661 aagattatat agagatagtt ccacttacag ttatttttaa tgatagggaa tatatagaag
     3721 gagtagatat aaagaaagaa gagttttata aaaaagtaaa agaaatcaag caaataccta
     3781 aaacttcaca agctacatat atggagttta aagaagtgtt tgataagttc ataacagaag
     3841 gttatcatat aatctgtatg acaggggctg caaacgcatc aggaactttt caaagtgcta
     3901 tgatagctaa aaatgatgta aatgaaaatg aaaaaataca tatatttgat actagaaatt
     3961 tatctctagg tagtggacaa tatgttataa aggcatgtga acttcttgaa gaaggactgg
     4021 gatttgaaga aattatagat gaattagaaa atactcgcag tagtgtaaga ttgctttttg
     4081 caccatatac acttgatttt ttaaagcaga gtggaagagt tccagttgca actgctttga
     4141 taggaaatat gttaaatata aagcctatat ttttctttga taatggggaa gctaagttgg
     4201 taaataaggt tagaggaata aagaatatag caagtaaatt ggtcgatata attttagaaa
     4261 tgaatgaagg gtctttagaa ggaaaaatcg tcacaatagg ttgtggagat aatttacacg
     4321 attgtgaaat attaaaagat gaagtaaata aaagaataaa agcaagaaga gtattattta
     4381 ctcgaggagg agtttctatt tgctctcata cagggccaga tatattggct ataagttgtt
     4441 ctaattagta gtaatataag gtttttgcat atatatgtga agataatttt atattgttaa
     4501 tttatagtat ttttgataat atagtaaata gagtgatata cgtgtttttt aataagagga
     4561 ggacaagcat taatgtttga acataaaata tttaaaatgg attttgcagg aagagaactt
     4621 tctgtagaaa taggaaagat ttgtgaaatg gctagtggta gttgtatagt tcgatacagt
     4681 gatagtatgg taatggtaaa tacaactaaa tcagccaaac ctagagacgg aatagacttt
     4741 tttcctttaa gtgtggatta tgaagaaaaa ttatactctg taggtaagat acctggaggt
     4801 tttttaaaga gagaaggcaa gccttcagag aaagctatac ttacatcaag attgatagat
     4861 agaccaataa gacctttatt cccaaaagga tttagaaatg atgttcaggt agttgcaaca
     4921 gttctatcag tagaccaaga ttgtacacca gatatagtag caatgatagg ttcttcaata
     4981 gccctttcta tatcagacat accatttaat ggaccaacag gttcagtatg tgttggcctt
     5041 gtagatggtg catttgttgt aaatccaaac gcagagcaaa gagaaaaaag ttcaatgcat
     5101 ctagttgttt caggtacaaa ggaagcaata atgatggttg aagccggagc tgatgaagtt
     5161 ccagacgagg taatgcttga tgctatttta tttgctcacc aagaaataaa aaaaatagtt
     5221 gagtttatag aaggtatagt tgcagaagtt ggaaaagaaa aaatgcctgt tgaattatac
     5281 catgcaggag aagaaataac tcaattggtt cgcgaatttg caactgataa gatgaaaaaa
     5341 gcagttcaga cttttgaaaa gttagagaga atggaaaata tggatagagt taaagaagaa
     5401 actttagctc actttgaaga aactttagaa gattttgaag attttgttgg agatatagaa
     5461 gaagtattac aagatataat aaaagaagaa gtaagaaaac ttatagtaca tgaaaatgta
     5521 agaccagata atagaaaact tgaagaaata agacctatat ggtgtgaaac tggcatgata
     5581 cctagagctc atggttcagc catatttact agaggacaaa ctcaagtact taatgttgct
     5641 actttaggtg ctttaggaga tgttcaaaaa ctagatgggt tagatgaaga agaaaacaag
     5701 agatatatgc atcattataa tttccctgca tatagtgttg gagaagctag accatcaaga
     5761 ggaccaggaa gaagagaaat aggacatggt gctttagcag aaagagcttt attaccagta
     5821 ataccatctc aagaagaatt tccttatgct attagattag tttctgaagt attaagttca
     5881 aatggttcta catctcaagc aagtgtatgt ggctcaacat tatctttact tgatgcaggt
     5941 gtacctataa aagatatggt tgcaggtata gcaatgggac ttataaagca tgatggtaag
     6001 gttgcagtgc tttcagatat acaaggtatg gaagaccatt taggcgatat ggactttaaa
     6061 gtagctggaa cagagtatgg tataacagca atacaaatgg atataaaaat tgatggtata
     6121 gataaagaaa ttctacaacg tgctttaaaa caagctaaag agggtagaat ccatatacta
     6181 ggagagatga gaaaaactat atctcagcca aaaccagaac tttcaccata tgcacctaag
     6241 attgttaaaa tgcaaataaa tcctgacaag ataaaagatg taataggacc tggtggaaag
     6301 ataataacta agataataga cgaaactgga gttaagatag atattgaaca aactggggaa
     6361 gtatttatat caggtattga gattgatatg attaagaaag ctcaagaact tataaataat
     6421 atagtagttg aaccagaagt aggaaaaact tataaaggta aggtaagtag aataatgaac
     6481 tttggagcat ttgttgagat acttccagga aaagaaggat tacttcatat atctcatata
     6541 gctcatgaaa gagttgccaa agttgaagat gtattgaata taggggatga agttgaagtt
     6601 aaagttacag agattgatga aaaaggaaga gttaatttat caagaaaagt tcttttacca
     6661 aaaccagaac ataaaaataa ataacttatt atataattaa aagcaaagtc ttaggatttt
     6721 gcttttttaa atacaaaaaa gtagataatt ttaaaatgat agttatggat ggcttatagt
     6781 tctttttctg tcaggcttta ctcttataaa gaagctattt aaaagctgat taatagagag
     6841 ctttattgat atatatagaa tcacttaagt tatatttagt gcataaacag agcttatttt
     6901 taatagtatt aaataagaaa caatttgtac ataattaggg ggagttttaa tgataatgat
     6961 ggtacttaat aagagtaagc tcaaaaaaat cataatacta attttagtag tgttattagt
     7021 attatcagga ggggtaatgg tattaaagtt atttaacaac aagccagtat ttaatttatt
     7081 taatggtgta gatttaccat atgaaagagg tacaaaggat aaaagtggat atgttgcatt
     7141 aacctgtaat atagaccttg gatgggaaac agagtacgta gaatccattt tagaaacact
     7201 taaaaaagaa aatgtaaaga ttacatttaa tgtaactgga aaatgggcag aaaagaataa
     7261 ggatgaatta ttaaagataa aaaaacaagg acatgaaata ggtaatcatg gatacaaaca
     7321 tttagattat tctacattat catatgaaga caattatgag caaatagaga cctctaaaaa
     7381 gataattgaa gaaattatag gtgaaaaaac taaatttttt caagcaccag caggttcttt
     7441 tggtccagaa acagtaaagg ctgctaaagc attagggtat acttctatca aatgggatgc
     7501 cgatactata gattggaaat acaaagacca accagaggta atcattgata gaatgaagaa
     7561 aaaagacatt aaagatagta gtataatttt aatgcatcca accaacgcaa cgacaaaatg
     7621 tatagatgat attatagcta ttgtaagaga aaaaggacta aaaccaggaa aacttagtga
     7681 tgtatttaag tagacaaagt ataaatattt ggttaagatt gtataagttg tagttgaatt
     7741 aattaaaaga ctgacttata atgtaataca tataaaaaat aaatataatt aaaacatagt
     7801 gttttcaata atccaatatg aaacaaagaa taaattgtta ggagaatgta atgtataaga
     7861 caaaaatact ggaaaatggg cttacaataa ttggtgaaga aataccatat ctaaagtcaa
     7921 taacattagg tatttggata aatgcaggct ctaggataga agaagcacag gtgagtggaa
     7981 tttctcattt tatagagcat atgatgttta aaggaaccaa aaacagaaca tcaaaagaga
     8041 ttgctagttc aatagataat ttgggtggtc aaataaatgc atttacaagt aaagaatgta
     8101 catgttatta tgtaaaacta atagatgagc atatagatac aggaattgat gtattgagtg
     8161 atatgatact aaattctaaa tttgataaaa atgatataga taaggaaaga cttataatat
     8221 tggaagaact taagatgtat gaagactctc cagatgactt atcttatgat ttattagtgg
     8281 aaaatatata tgctaatgat ggattaggaa tgaatataat tgggacaaaa gaatctctgt
     8341 acaatataac tagagaatcc atgttagaat acttaaataa gtattatatt ccaaataacg
     8401 ctgttatttc tatagctgga aactttaact ttgatgatat ggtggagaaa atcaaatcaa
     8461 aatttggaca ctgggaaaag aaaaatttaa gtatagacat aagtgaggct aagtttaatc
     8521 cttgctttat atcaaaaaat aaagatactg aacaggtgaa cttagctatg tgcctaaaag
     8581 gtattccttt tgaaaatgat gaagaagtct attcaatggc agtggtaaat aatattttcg
     8641 gtggaagtat aagctcaagg ctttttcaga aaataaggga agaaaaaggt ttagtatatt
     8701 caatatactc atcacagaca ttatatagga aatgtggtga attaggtata tttgcaagca
     8761 tgagtacaga aaatttacaa gatgtatata atttaataaa gaaagaaatt gaaaatataa
     8821 gagaaaacta tcttacagaa aaagaaatat ctgaaagcaa ggaacagtta aaaggaaatt
     8881 atatattgga tttagaaagt actagcagta gaatgatgtc tactggtaaa tctatgttac
     8941 ttagcaaaaa agtaaaaact acagatgaaa tacttgaatg tataaataat gttaatatta
     9001 attcaataaa gaaggttgtt gataaggtat tcaatatcga aaatattggt acatgtatag
     9061 ttggaagaga tgtagaaaag attctacact tggattaata ttgaaaaaag taaatctctg
     9121 gaggtataaa atatgaattt atctgaaata gcaggtaagg aaatagttaa cttggtcaca
     9181 ggagaaagat tgggggtcat tggagaatgt gaccttatac tagatgaaac tacaggcgat
     9241 atattagcgc ttctaattcc aaaagaaaaa ggattttttg ccagaagaaa agataagtca
     9301 tttttagaag taccatggag aaatgtaaaa aaaataggta atgacatgat aattatagaa
     9361 tatgagggat atatttagga ggaaaaaatg agtattttag tgcaaaagtt tggtgggaca
     9421 tcagtagaat catatgaaaa aatgaatgaa gtatgtaaga tagtaaaagc atacaaaaaa
     9481 aatgatgaag aattacaatt agtattagta gtttcagcaa tgggaagaaa aggagctcca
     9541 tatgctacag atacacttat aaatttgtgt agtgcagtaa atgatgagcc aagtaaaaga
     9601 gaacttgatt taataatgtc ttgtggagaa ataatatcag gaacaattct tgctaattta
     9661 ttgaatgcac aggggataga ttctgttttc tttactggtc agcaagcagg tataattaca
     9721 agtgatgaat attctaatgc aaagataaag tatataaatc ctaaaaaggt aaaaagagct
     9781 ttagatgatg gtaaggttgt agttatagct gggttccagg gtgtaacaga tgatggagaa
     9841 ataactactc ttggaagagg cggaagcgat acatcagcag ttgctattgg taaagcttta
     9901 gaatgtgaaa cagttgaaat ttacactgat gtggatggta taatgactgc agaccctaga
     9961 gtagaaccaa acgctaaagt attgagtttt atagactatg aggaagtatt ccaaatggct
    10021 gacaaaggtg caaaagtaat acatccaaga gcagtacagt tggctaaaag tggaaatata
    10081 actttagcca taaagaatac aatgaatcca acttttgaag gtacaaaaat aggttcttta
    10141 tgtagacact tagaagacaa tatagaatat gaacaagaaa gagattttaa ggttgctgtt
    10201 gcaaataaag atagcgtagc acaagttaag attaagtcag cagaagaagt gtttacagaa
    10261 gtattaaatg agatagaaaa gaaacttata actatagata tgataaattt ctttatatca
    10321 gaaaaagcat ttgttgtaga agatgctgat ataaaatcat taaaggaaat actagataaa
    10381 tttgaattag attatgaagt aaatagagac tgtgctaagg taacattgat atgttcaaga
    10441 attgctgatg aaatgtctgg tatcatgtca aaggtagtta gaggattatc aaaagcaggg
    10501 gtatctcttt tacaaacatc agactcaaat atgactatat cttgtttagt tagtgaagaa
    10561 gatatgcata cagcagtaca tgctatacac caacaatttt atttaaaata aactgtataa
    10621 tacaattatt ttttaatcaa atttaagaat ttcatatata tcttaatata tttgatttat
    10681 ttggtaaaac taaattaaat ataattttga taggagtttt tttatgaaag attatagaga
    10741 agataatgaa aaatttgata aagataattg tagtgaagaa tctagttgcg aagagagaga
    10801 atgtataaag caatttggta ccaatgaaat gccacctcaa cctcctaaag atattcaatg
    10861 tataactata attggagaaa ttgagggaca ttttatagga aatcctcaaa aaaaagctac
    10921 aaagtacgag catataattc caatgctata ctcaattgag gaaagtaatg atgttaaagg
    10981 agtgttagtt gttttaaaca ctgttggagg agatattgaa gcaggtttag ctattgcaga
    11041 acttttaaac agtacatcta aaaaagtagt tactcttgtt ctaggaggaa gtcatagtat
    11101 aggtgttcct cttgctacag caggagatta ctcatttata gcaccaacag caaccatgat
    11161 tatacatcca gtgcgaacta ctggtcttgt gattggtata aatgagactt ttgaatattt
    11221 taaaaagatg caagatagaa taattcaatt tataataaga acatcaaata tcaaaaaaga
    11281 tgtacttgaa aaacttatgc atgaaaaaga tgaacttgta agtgatgttg gtagtgtatt
    11341 aataggaaaa gaagctgtgg attatggtct tatagatgaa gttggaggac taaaagaagc
    11401 acttaaaaaa ttaagagagc ttataaaaga atctgaggaa gaaaaaaata atgattaaaa
    11461 tttgtttaaa taataaaatt tatgatataa tgatgtaata aagtaggagt gttagtgtta
    11521 aggcattagc actttttgct gtgaaggggt aaaacttgct aaaaataagg agtgatatta
    11581 atggccacga aaaagaagaa aaaaaagaag gtaaatacct taagttttaa tgcagaatat
    11641 cataatttaa taacaatatt tataggtgta tttttattat atagtttaaa ttctaattct
    11701 atgggatgga tacctgtgtt aatgcaaaat ctttttaagg gactatttgg agggctttca
    11761 atagcaatac catttatagt aattataaca ggacttttag gattttttga tggaaatgaa
    11821 tatatatata ggctaagaaa gactaaacta tactacataa ttattttatt tatatttgta
    11881 ttttatggat tattaaatgc aggaactctt cctgtagata gcccactaaa aggtaacatg
    11941 tttgatgatg ttatgaaatt gggtgtaagt ggacaaggta gtggtttgat agcaacaact
    12001 attgcgtatt atatgagtaa aatatttggt atagcaggtg gatggttaat aagtatcttc
    12061 gcacttatac tttctgtaat gtttatattt aatatttcta taaaagattt ggtgtcaaat
    12121 gcaaaatcaa aatctaaagc atctaaagac agcaatttaa cgtttaaaga taaaattgca
    12181 aatatgaaaa aatctgcaat agatatgatg actgatgaag tagatgatac tactataaat
    12241 aaaaaaccag gtttctttaa agggttaatg tcaaaaggtc ttaataatga tgatgaagaa
    12301 gatgaatatt ttgaagaaga aaatagtgat ggtgtagatg ataaaactat aaaaatagtt
    12361 ggatttaata aggcagaaga tgagtattta gaaatattag aaggaactca aagtatgcca
    12421 gagttagatg ttttaaaaga attacaaaaa gctaccaatg aaaatccagt tgtagatact
    12481 aaaccagaaa aaaaagttga tatagctaaa cctaatttaa atatagaaaa aactcaacct
    12541 atgagtattg tagcagagcc agttaatgag gattattcaa actataaaaa gcctagcatt
    12601 gagcttttaa ataaagtaaa taagaagtct gatgaaaatg gtaaaaagaa agtgttgaaa
    12661 aatgcttctc ttttagaaaa gacactatca gattttggag tggaagcaaa aattaatcaa
    12721 gttacagttg gacctactat aacaagatat gaaatacaac caagtccagg agtcaaggta
    12781 agtaaaattg taaatttaac tgatgatata gctttaagtt tagcagctaa aagtataaga
    12841 attgaagccc caattcctgg taaaagtgca attggaatag aagttccaaa tgaagaagct
    12901 caaatggttg gtgtgagaga agttctagaa agtgaagaat ttaataattt taactctcct
    12961 cttgcaatgg gacttggaaa agacgtagca ggaaaaatta taattggaga tataggtaag
    13021 atgccacact tactaatagc tggttctaca ggttcaggaa agagtgtgtg tgtaaataca
    13081 ttaataagta gtattttata taaggcaaat cctgatgaag taaaattact attaatagac
    13141 ccaaaagtag ttgaacttgc aaattataat ggaataccac atctattgat accagtagtt
    13201 actgacccta aaaaagctgc aaatgcttta aattgggcag ttacagagat gaatagaagg
    13261 tacaaattat ttgcagatgc tcaagtaaaa gatgtaacaa gttataatga gaaagcggaa
    13321 gaaaaacttc ctaaaatagt aataataata gatgagttag cagatttaat gatggcaagt
    13381 gcaaatgatg tagaagatta tatttgtaga ttagctcaaa tggcaagagc tgctggaatg
    13441 cacttgattg tagcaactca aagaccatct gttgacgtta taacaggggt aataaaggct
    13501 aatataccat ctagaatagc atttgcagta tcatctcaaa ctgattcaag aacaatcctt
    13561 gatatgggtg gtgcagagaa gcttctaggt aaaggtgata tgttattcta tccacttggt
    13621 gcagcaaaac cagttagact tcaaggggca ttcatatcag agagtgagtc ggaaaaagta
    13681 atcgattttg taaaaagtca ggttaaagat ggaataaaat atgaagaaga tataattgaa
    13741 actatatcta aggttaatac ttcaaagggt agtgatgagg atgagttttt gagtgaagcg
    13801 atagaatttg tcgtggaaag tggtcaagca tctgcctcta tgctacagag aagatttaaa
    13861 ataggattca atagagcagc tagacttata gattctatgg aagaaagagg aataattgga
    13921 gcaagtgagg gaagtaaacc gagaaaggta ttaataagca aacaggattt gcagaattta
    13981 gaaggtgaat agaattgtca gttataaaaa tagaaaaagc acttaagcta gataaagtaa
    14041 tatttgtaga tgtgagaact gaaggagaat atgaagaaga ccatatattg aatgcaatca
    14101 atatgcctct ttttaaaaat aatgaacata atgaagttgg aacaatttat aagatgcagg
    14161 gaaaacatga agcaatacaa aaaggatttg actatgtatc gtataaatta aaggatatat
    14221 atttacaagc agcagaactt gcattaaatt atgataatat tgtcatatat tgtgcaagag
    14281 gcggaatgag aagtggcagt atagtaaatt tattatcatc attaggtgta aatgtatatc
    14341 aacttgaagg tggatataag gcttatagaa attttgtttt agagtattta aggcatgtaa
    14401 tggatacaaa aaactttgta tctgttcatg gattgactgg tacaggaaag actgatttgt
    14461 tacatttatt agatgaaaaa ggaatagacg tgctggattt agaaggcata gctaaaaaca
    14521 gtggttctac ctttggtttt ataacttttg ataaaaagcc accatctcaa aagaactttg
    14581 aaacaaaact atttgaaagt atattttttt cgaaagagaa ttatatattt gtcgagagtg
    14641 aaagcaaacg agttggaagt gttacagttc caaatgaaat tttcgaggca atgacacgag
    14701 aaggacatca tatattatta gaatgtgata ttgaaaatag agttgataga ctttgtagag
    14761 attatattta tgataaagat gaaggtaata tattgatttt aaaagaatgt ataaacaagt
    14821 ttagaaaaag attgggtaat aaagtagtag atgattatat tgatttatta gaatcaggtg
    14881 aatataaaga acttgtaaaa agatatcttt tagaatacta cgacccactt tatatgcatt
    14941 cagtagagaa atataagtat agtaagatta taaattttaa tgatacacaa aaagctttag
    15001 aagatgttat agacttatat gagagtatat tggaaggaga aagtgaatgt taaaaatagc
    15061 attggaatca ttaggatgct caaaaaattt agtagatgca gaaataatga tgggaatttt
    15121 aaataacaaa ggctataaat taattggaga ttttgaagaa gctgatgtaa ttatagtaaa
    15181 tacatgtggg tttatagagt cagctaaaca agaatctata gatacgataa taaactttgc
    15241 agagttaaaa aaaactggca atttgaagtt gttgatagta acagggtgtc tagcccaaag
    15301 atattctgaa gaattaaaaa ctgaaattcc tgaaatagat gctatagtag gaacaggaag
    15361 ctatcaaaat atagataaaa tacttaaaga attaagtgaa atgcatcaaa ttgtaagcct
    15421 taatgatata gagtttgtat ttaatgaaga tttaccaaga tatatatcta cacctagcta
    15481 tatggcctat ttaaaaattg gagaaggatg ttccaataat tgtacttatt gtataatacc
    15541 aaaactaaga ggtaaatata gaagtagaaa atttgaagat ataattaaag aagctaagaa
    15601 attagctgaa tctggagtaa aagaacttgt ggttatagca caggatacga caaagtatgg
    15661 ttttgattta tatggaaaag aaagattatc agaactttta gaagaacttg caaaaataga
    15721 tggatttaag tggataaggg ttatgtattc atatccagaa tctataacag aagaattgat
    15781 acaagttata aagaaatatg ataatatatg tagttacttt gatatgccaa tacagcatgc
    15841 aagtaacaat attttaaaat taatgaatag aaaaactact aaagaagata tattaaataa
    15901 aatcaatctg ataagaagta atataccaga tgctatactt agaactacaa taatagtagg
    15961 gtttcctggt gaaacagaag atgacttcaa acaacttgtg gactttgttg aagaggttaa
    16021 atttgataga cttggtgcat ttgcatattc aagagaagaa gatacaccag cagatagatt
    16081 gccaaaccat atagatgaag aagtaaaaat acaaagaaga gatactttaa tgatgattca
    16141 acaaaaaata tctgaagaac ttaatgacaa aaagatagga aaaacttatg aggtgttaat
    16201 agaagaacag atagaagata atgtatatac aggaagaaca caaggtgatg cagaagaaat
    16261 tgacagtata gtgtatgtaa aaagtgtaga caatcttgaa gtaggagaat ttgtaagtgt
    16321 tcaaataaat gatgcaatgg aatacgattt aatgggagat gttttatatg aacttgccta
    16381 acaagttaac tttgtttaga atatttttaa taccagtatt tgttttaatt atgttgctca
    16441 atgtaccaaa taaattttta atagcttgta ttatttttat aatagcttca attactgatg
    16501 ctttagatgg aaaaatagct agaaaataca atctagtcac tgactttgga aaatttatgg
    16561 acccattagc agataagtta ttggttattt cagcacttac atgtatgata gaagaccatc
    16621 ttgtaagtag ttggatggta attataatag tggctagaga gcttacagtt agcatattaa
    16681 gagctatagc tgctgctgat ggaaaagtaa ttgctgctgg aaacagtgga aaacttaaaa
    16741 cgataactca aatggtatct atagtgtttt tattgttagg tgctcagttt gaaaatgtat
    16801 tgatattaaa tattggtgaa attctcattc ttatagctac actacttaca ttatattctg
    16861 gatgggaata tctatataaa aataaagagc tttttatgtc ttctaagtaa aaaattatgc
    16921 tttaataaat aaaaactaat gattaataaa taaaaaataa caacttaaaa ttttaaaaat
    16981 gtaaaaaagg gtatactatt atagtatacc tcttttcatt ttaagaatat tttcttttgt
    17041 gtatataatt attaataatg gtttataaat aaaatgtaat gaataaaaat ataatcatta
    17101 gtagcgtttt taaattgact aggtttgatt tatgttatat aattaatata taacaagaga
    17161 acaaatgttt gtagataaat gaagggatgg tatagagatg agtgtagatc aagaaaaatt
    17221 aaaagcgttg aatgaagctt taggtaaaat tgaaaaagat tttggtaaag gttcagtaat
    17281 gaaattggga gaagcaacat ctatgtctat agatgttata tcaacaggag cgattggttt
    17341 agacatagct attggtatag gaggtctacc tagagggaga atagttgaag tatatggtcc
    17401 agaatcttct ggtaaaacta ctgttgcgct tagttgcgta gcatcagctc aaaaagatgg
    17461 aggaatagct gcatttatag atgcagaaca tgcacttgac ccagtatatg caaaagcttt
    17521 gggtgtggat gttgataacc taataatatc tcaaccagat acaggtgaac aggctttaga
    17581 gatagcagag gcattgataa gaagtggagc gatagatata atagtaatag actcagtagc
    17641 agcattagtt ccaaaggctg aaatagatgg agatatgggt gattctcatg taggtttaca
    17701 agctagactt atgtcacaag cacttagaaa gttaactggt tcaattaaaa aatcaaattg
    17761 tgttgctata tttataaacc agttaagaga gaaagtagga ataatgtttg gaaacccgga
    17821 aactactact ggaggacgtg cactaaaatt ctattcatca gttagattgg atgttagaaa
    17881 aatagataca ataaaacaag gtgataaagt tataggtagt agaactagag ttaaagttgt
    17941 taaaaacaaa gtagcaccac catttaagca agctgaattt gatataatgt atggagaagg
    18001 aatttcaaaa attggagacc ttttagatat agctgctgat gtagatatag ttaaaaaatc
    18061 aggttcatgg tatagttaca atgatactaa acttggacaa ggaagagaaa atgttaaaaa
    18121 attcttggaa gataatttag atttaactac tgaaatagat gagaaagtta gagcatttta
    18181 caatttaaat gaagaacatg aagaatcagg tatttcagta tcaaaagaaa ttgtagaaga
    18241 ataagtaaaa atacaaaaaa ataactagta attaaagaca tgccacgctc cctattagct
    18301 tgacacaatg ttgaaaaaat tataaaatta agtgaggata aaatatctaa atttaatttt
    18361 aattattagg aatatttttt aatcatagtg atgacatttt gggattatta acgaaggagg
    18421 tggatgtcat agatagtgta gtaatgattg taataggtgc agctgttggt attatagctg
    18481 gatattttgt tagaaaaaat atatctgaag caaaaattgg acaagctgaa aacttggcca
    18541 aagaaataat agataaagca caccacgact cagaaacagt acaaaaagaa aaattactag
    18601 aagctaaaga agagatccat aagtggagaa cagaagctga aagagaaaac agagaaagaa
    18661 gaacagaagt acaaaagtat gaaaaaagag tagtacaaaa agaagaagtc ttagatagaa
    18721 aattacaaaa tttagaatct aaagaagtaa acctaagtga aaaattaaag gtagtggaaa
    18781 agaaagaaga agaagttgaa gtaataaaaa ctcaacagtt agaaaaatta gaaagtatat
    18841 caggaattac ttctgataag gccaaagaaa tcatcttaac caatgcggag agagatgtaa
    18901 gacgtgaaat gtctataatg ataaaagaga tagaaagtca agctaaagaa gaagctgata
    18961 agaaatcaag agaaattata ggatatgcaa ttcaaaaatg tgcagcagat cacgtagccg
    19021 aaactactgt aacagtagta agcttgccta atgatgaaat gaaaggtaga ataataggta
    19081 gggaaggtag aaatataagg actttagaaa ccttaacagg tatagacctt ataatagatg
    19141 acactccaga agccgtaatt ttatcaggat ttgatccaat aagaagagaa attgcaagaa
    19201 ttgctctaga aaaattaata gcagatggaa gaatacatcc agctagaatt gaagaaatgg
    19261 ttgaaaaggc gagaaaagaa gttgacaata ttataaaaga atatggtgaa caagctgcat
    19321 ttgaaactag tgtacatggg attcatccgg aattaataag attattaggt agacttaact
    19381 atagaactag ttatggacaa aatgtactta aacattctat agaagttgct catatagctg
    19441 gtataatggc tgctgaaata ggagcagata ttagattggc taagagggca ggtttattac
    19501 atgatatagg taaggctgta gaccatgaaa tggaaggtac acatgttgaa ataggtatgg
    19561 atttacttag acgttataaa gagtctaaag aagttataca tgccatgagt actcatcatg
    19621 gagattacga gcctcaaaca gtagaggctg tattagttac agcagcagat gcaatatcag
    19681 ctgctagacc tggtgcgaga agagaaactt tagaagcata tataaagaga cttgaaaaat
    19741 tagaagaaat agctaattca tatgaaggtg tagaaaaatc atttgctata caagctggta
    19801 gagaaataag aataatggta aaaccagaag ctataaatga tgaagaaata catctattgg
    19861 ctagagatat gactaagaaa atagaggatg aacttgaata tcctggacaa ataaaagtaa
    19921 gcataataag agaaacaaga gctatagagt atgcgaagta aaagaaataa tatctaaata
    19981 ataagaaaat atttttagaa atttaaattt gccacaagat atgataatat atcttgtggc
    20041 ttttaaattg tttaagttta attttatata atgcaaaaat aatctaagtg ctaaaagtgc
    20101 tacttacaaa agattaataa aataaagctt tatatatgta ttatttatat aattatccaa
    20161 gtttgtt
//
LOCUS       DAMJVI010000070        20135 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.072, whole genome shotgun sequence.
ACCESSION   DAMJVI010000070 DAMJVI010000000
VERSION     DAMJVI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.072"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(314..775)
                     /locus_tag="PC545_002507"
     CDS             complement(314..775)
                     /locus_tag="PC545_002507"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDF2457272.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVSP
                     ENIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDSELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLD"
     gene            complement(880..2073)
                     /locus_tag="PC545_002508"
     CDS             complement(880..2073)
                     /locus_tag="PC545_002508"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDF2457273.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDESRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINIENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRIPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            complement(2096..2761)
                     /locus_tag="PC545_002509"
     CDS             complement(2096..2761)
                     /locus_tag="PC545_002509"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDF2457274.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTILLTKNVGDFVNLESDVIGKYVNNFMANNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            complement(2795..3907)
                     /gene="ribD"
                     /locus_tag="PC545_002510"
     CDS             complement(2795..3907)
                     /gene="ribD"
                     /locus_tag="PC545_002510"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDF2457275.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDSNI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLKDNNIDVTVGILEDEARDLNEAFFYHIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESIKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNKLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLTINNVTLIDGDILIESDVLN"
     regulatory      complement(4039..4160)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4349..6831
                     /gene="recQ"
                     /locus_tag="PC545_002511"
                     /pseudo
     CDS             4349..6831
                     /gene="recQ"
                     /locus_tag="PC545_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729557.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
     regulatory      6920..7031
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7173..8474
                     /gene="thiC"
                     /locus_tag="PC545_002512"
     CDS             7173..8474
                     /gene="thiC"
                     /locus_tag="PC545_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDF2457276.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKIKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            8528..8722
                     /gene="thiS"
                     /locus_tag="PC545_002513"
     CDS             8528..8722
                     /gene="thiS"
                     /locus_tag="PC545_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDF2457277.1"
                     /translation="MKVNGKEIEFEKDLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            8715..9518
                     /gene="thiF"
                     /locus_tag="PC545_002514"
     CDS             8715..9518
                     /gene="thiF"
                     /locus_tag="PC545_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HDF2457278.1"
                     /translation="MVKIKIFVNELLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMTLCENDKLTLIQKGVKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DIFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            9581..10351
                     /locus_tag="PC545_002515"
     CDS             9581..10351
                     /locus_tag="PC545_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDF2457279.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPVQMA
                     RAFKLAVEGGREAYIAKTGNVSEFANASSPLTGFLGNL"
     gene            10368..11471
                     /gene="thiH"
                     /locus_tag="PC545_002516"
     CDS             10368..11471
                     /gene="thiH"
                     /locus_tag="PC545_002516"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDF2457280.1"
                     /translation="MSFYDVIEKYRDFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEDMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPHIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            11472..12161
                     /locus_tag="PC545_002517"
     CDS             11472..12161
                     /locus_tag="PC545_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDF2457281.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELRK
                     LYMKIKTKINCIDFQEQISDESLKTNINQKECRNKFKVNFIINSNIEFFEKVDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVEEVDYLEKLIRNQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAIMSTIMRSKDIK
                     KTISNYNEKLN"
     gene            12349..13743
                     /gene="yfcC"
                     /locus_tag="PC545_002518"
     CDS             12349..13743
                     /gene="yfcC"
                     /locus_tag="PC545_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDF2457282.1"
                     /translation="MTDSKKKRFIIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFATIGVLSGLVGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            14260..15627
                     /locus_tag="PC545_002519"
     CDS             14260..15627
                     /locus_tag="PC545_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDF2457283.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSIKNNLTMIDNQLLSIYKNFH
                     SGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFVVDFKKPCETILNYSFNKQDIS
                     DEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTFDDKFCKDLGNHFRSRLNTEFP
                     WNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENREQLLCQFEEDSKLIIEDIYDC
                     IFNSISEIDSVEILYNYNLEKINFTSSEIINCICREYECYGEYLEEKEKLMNTFKEKI
                     IQIFE"
     gene            15652..16260
                     /locus_tag="PC545_002520"
     CDS             15652..16260
                     /locus_tag="PC545_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDF2457284.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGRNSRMGRDKAFLE
                     LHDKLFIEIAIEAFKNFDELIIISNNEELYSKYDIKVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNNQIVERIANKMNDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYEKLTKV"
     gene            16308..16679
                     /locus_tag="PC545_002521"
     CDS             16308..16679
                     /locus_tag="PC545_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDF2457285.1"
                     /translation="MNLLKCDSWAVILNNSDKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            16858..17196
                     /locus_tag="PC545_002522"
     CDS             16858..17196
                     /locus_tag="PC545_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDF2457286.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            17662..18165
                     /locus_tag="PC545_002523"
     CDS             17662..18165
                     /locus_tag="PC545_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HDF2457287.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNKIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEVLRGEATE
                     CATKKSE"
     gene            18212..19171
                     /gene="moaA"
                     /locus_tag="PC545_002524"
     CDS             18212..19171
                     /gene="moaA"
                     /locus_tag="PC545_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDF2457288.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKIRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRIFELEKCGLNKVNISLDSLKEYKYKSVTRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            19175..19645
                     /gene="moaC"
                     /locus_tag="PC545_002525"
     CDS             19175..19645
                     /gene="moaC"
                     /locus_tag="PC545_002525"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDF2457289.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDVDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            19681..20112
                     /locus_tag="PC545_002526"
     CDS             19681..20112
                     /locus_tag="PC545_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDF2457290.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENVTTEGIEVFSLPVGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVKVLKEGIINVNDSIDVI"
BASE COUNT         7843 a         2194 c         3435 g         6663 t
ORIGIN      
        1 aaaaagtaat aaaatagata attaatatac tagtaatata tgctataaat aaaaacaata
       61 gctaaaatag taatatatat gtttttatga tattttatgt ttagggttat atttgtaatt
      121 attttaagct tctaaaagta gatttgatgg ataacgtata attttagaag gatatgcaat
      181 ttgtttgata agaacgtaca tatgattaaa ttaaaaatat taagtaccaa aaagaagaaa
      241 gatagtttct ccctttggta cttaatattt tgtatccttt acactaattt ttaatattta
      301 ttaatgatat tatttaatct agagacttaa ataaatttgc catttctagc gcattcatag
      361 cacaatcata acctttattt ccagcttttg tacctgctct ttcaatagct tgttcaatat
      421 tttctgtagt tacgattcca aatataacag gtaattcact gtctaaagat actttggcta
      481 tccctttaga gacttcacta catacataat cataatgaga agtagcccct cttataacac
      541 agcctaaaca gattatagca tcatacttac cacttttagc cattttttga gcaacaagag
      601 gaatctcaaa agctccaggt acccaaacta tttctatatt ttctggagaa acgtcatgtc
      661 ttaaaagaca gtcttcagct cctgataaaa gttttgatgt tataaattca ttaaaacgag
      721 aatttattat acctattttt aaatcttttc caataagttt tccttcatat atcattttta
      781 cttcctccaa ttttatttat gttatagatt tatatttata aatatcttta atttaattat
      841 aaaaaactac ttagtagaaa aatatcatca tataatcatt tatataatat ctaaaagatg
      901 tcccatttta tcttttttag tttttaaata tagcttatta aagtcattaa tttcttcatt
      961 aatagggatt ctattttcga tttttattcc atatttctct aattgattta ttttatctgg
     1021 gttattactt aaaagattta tactttttat gtttaaattt tttaatattt gagcagccat
     1081 atagaaatct ctcatatctt cttcaaatcc aagcattaaa tttgcttcaa cagtatcgta
     1141 accttcttct tgaagtttat aagccttaat tttatttaaa agaccaatac ctctaccttc
     1201 ttgcttcata taaattaaaa ctccacttcc attttccaca attttcttca tagaagattc
     1261 tagctgtaat ccacaatcac attttagaga gtggaacaca tctcctgtta aacattcaga
     1321 atgaagtcta actagagtat tttctatatt tatatttcca taagtaaggg caatgtgttc
     1381 ttcatttgaa taagtatctc tatatcctat gatttcaaaa tttccatatt ttgtcggaag
     1441 gaaagcacta gatacttttt ctatagtagt ttcatttatt ttgcggtatt ctataaggtc
     1501 ttttatagtg atgattttta aattatgctt ttctttgaaa atcattaaat catcaactct
     1561 tgccattgta ccatcatctt tcattatttc acagataaga ccaatctctt taaaacctgc
     1621 aagtctagca aggtctactg aggcttctgt atgtccatcc ctgacaagaa ctccaccagt
     1681 ttttgctatt aaaggaaaga tgtgaccagg cgttttgaaa tctttacttg tagaattttc
     1741 atcacagaat aattttacag tttcagctct ttcatatgca gatataccag ttagagtatc
     1801 tgctccatct atagtaacag taaaggctgt ttgaaatgta tctgtatttt ctctaaccat
     1861 aagaggtata ttaagacttt taaatttttt ttcagtggca ggtagacaaa ttagacctct
     1921 accatatgta gccataaaat ttattgattc aggagtagcc atttcagctg ccatcaataa
     1981 atctccttca ttctctctac tttcatcatc aactacgatt accattttcc cattttttat
     2041 atcttcaata gcttcttcaa tagtattaaa catgtaattc actcctttat tatttttaaa
     2101 aaccattttt aaataaaaaa tctttatcta tatttgattt atggttacta ctattagaat
     2161 ttaattcctt gtagttatta gccataaaat tattaacata ctttccaatt acatcgcttt
     2221 ctaaattaac gaaatctcca acattctttg tcaagagaat agtgttaatt ttagtatgag
     2281 gtataataga aaccttaaat atttcatcat ctaaatatga tatagttaaa cttacaccat
     2341 caatagcaac agaacctttg agtatcattg aggataaaag ttttttatca ggtcgtatac
     2401 ttactaaaac tgcattttca tctttttcaa atttgcagat agttccttta ccatcaacat
     2461 gacctgttac tatatgacca ccaaatcttg aagaaagact catagctctt tcaagattta
     2521 ccttgtcatt tgcatttaat aaacctaaat ttgtggaacg aattgtctcc atcataacat
     2581 cggctgtaaa ttcatttttt ccgaggtttg aaacagttag acaaactcca tttacggcta
     2641 tactatctcc aagatttgtg ccatctaata ttttatttga tagtatggtt acttttccac
     2701 tcttaccatt agcagtaatt tttctaagta ttccaacttc ttctactatg ccagtaaaca
     2761 tcctaacctc ctatatgaat gtattaacaa aaatttaatt taaaacatct gattctataa
     2821 gaatatcgcc atctatcaaa gttacattat tgattgttaa ctgtgttgaa tctgacaaat
     2881 gatttattcc ttctccagaa acgaatgtag gtgcatcttt accacctata atcttgggtg
     2941 atagaaataa tttgacttta tcaactaact tatttctaaa gagactgtca ttgagagtag
     3001 caccaccttc aactagaaca gaatcaatgt ttaactctcc taatttatac attacaaact
     3061 ccaaatctac taagttgttt tttgaaggag aaattattgt ttcaacatta ttttctttta
     3121 acatagataa ttttatactt tctttgcaac atgtgaaaac tatagtttta ctggttttat
     3181 ctttaaccaa attactatta agtggcaact ttaggtgtgt atctattact attcttgtag
     3241 gatgagacac tttttcttga ttgagcctac aagttagtaa aggattatcg ttcaaaactg
     3301 tatttatgcc aaccattata ctttggtatt tatttctgta tttatgagtt agataccttg
     3361 atgattcatt agatatccat tttgattcta gtgattttgt ggcaatttta ccatctagac
     3421 ttactgctga ttttacaata caaagaggtc ttttgttttt tatatgataa aaaaatgcct
     3481 catttaaatc tcttgcttca tcttctaata ttcccacagt aacatcaata ttgttatctt
     3541 ttaatttttt tactccatta ccacaaacaa gtggatttgg gtctaatgta gatattacaa
     3601 ctctcttaat cttattttgt ataattttat ctacacaagg aggcgttttc ccataatgag
     3661 aacatggttc taagtttaca taaagagtag agtcttttag actttgttta gcactgttta
     3721 tagcatttac ttcagcatga ttagagccaa atttctcatg atatcctttg cctattatat
     3781 tactatcttt aacaattaca catccaacta atggatttgg atttacaaag ccttctccat
     3841 ttttagccaa ttcaatagct tttttcatat aataaatatc tttttctttt tgattcacaa
     3901 aattcacaat atgtacacct cctgtttaat tggataattt ttaaacaaaa aaccctgaga
     3961 attaactcag ggtttagaat aatctacaat aaatgtttaa taaatgtaga acaaaattgc
     4021 tctacaaaaa cactcattaa atatcttctt ccatccagac tttactgtcg gctttggaat
     4081 ttaaccaaat caaccaagtt ttacattggc tcgcgggcta taccgccgat tgggaatttc
     4141 accctacccc gaagattaag tattaaattt atatttagaa tgtatcataa attattttac
     4201 tttgcaaact atttttttaa aaaatatatg ttaatatata atatagaatg tctataaact
     4261 ttgaaatacc acgattgtga atataaaaat aaaaatttga acataatcag gaaagaagtc
     4321 taattaattt aaattaggag agtaatatat gaataaaaaa cttttagaaa tactatcaaa
     4381 atactatgga tatacaagtt ttagaaaagg tcaggaatct ataataaatt ctatcttaag
     4441 cgaaaaagat gtgctagcta ttatgccaac aggaggggga aaatcaatat gttatcaaat
     4501 tccagctttg attttagatg gaatgacaat agtaatttct ccacttatat cacttatgaa
     4561 agaccaagta gatgccttaa agactatggg aattagtgct gcttttataa atagttcatt
     4621 aagtagcaaa gaatttaatg aaatattaaa taatattaga aaaaataatt ataaaatact
     4681 ctatattgct ccagagagat tagatgcaca agagtttatt gagctaataa ataataacaa
     4741 tgtaagtcaa gttgcgatag atgaagcaca ttgtgtatct caatgggggc atgattttag
     4801 gttgagttat agaaggattt ctgattttat aaaaaatttg cctaaaagac ctatagttac
     4861 agcatttact gcaacagctt cagaagaggt aagaactgat ataataaatt tactgtgttt
     4921 agaaaatcca gattattata taactggatt tgatagagag aatttatcaa taaatatagt
     4981 aaaatcatct agtaaaaata agtatatttt agattatatt caaaatcata aaaatgagtc
     5041 tggaattata tatgttgcaa ctagaaagga agttgaaaat atatataatg gtttatctaa
     5101 gagaaatata tctgtatcaa aatatcatgc aggtttatct aaaaatgaaa gaagtaaaaa
     5161 tcaagaagat tttataaatg ataatgtgga tataatggta gcaacaaatg catttggaat
     5221 gggtattgat aaacctaata taagatgggt tatacattac aatatgcctc aaagtataga
     5281 aaactactat caagaaatag gtagagctgg tagggatgga gaaaaaagtg agtgtgtact
     5341 tttattttct ccaggagatg tacatataca aaaatattta attgatattg gagttgaaaa
     5401 tcctgaaaga aaattatttc agcataagaa gcttcaatac atgattgact tagtttacag
     5461 taattcatgc tatagaaaga caatattgag ttattttgga gaaaattata tagacaattg
     5521 caataattgt agcaattgtt tagtagaggg tgaaatagta gataaaacta tagatgcaca
     5581 aaaagtcatt tcatgtgtag ctagaatgaa aagaagttat ggtgttacta ctattgtaga
     5641 tgtgcttaga ggttctaaga ataaaaagat tttacagctt ggattaaata ctttgtctac
     5701 atataacatt atgagagatt attctagtga agacttaaag aattttgtaa atacattggt
     5761 ttctcatggt tttttagatt tagttgagac tctaggcaat ggaaatagag gtagttttcc
     5821 tactataaga ttaaatgaga tgtctatgaa agttttaaaa ggtgaagtga aagtagagtt
     5881 taaagaaatt gttatgacaa aatcattgga agtagaggat gagttatata atgctttaag
     5941 agaacttaga cattcaattg caagtgaaga aaggatagct ccatatatgg tatttggaga
     6001 tggtacactt agagccatgt ctagtagtta tccaattaat aaagaagaaa tgctagatat
     6061 ctctggagta ggagaaatta aataccaaaa atatggtaga gattttgaag cagtaattaa
     6121 agaatatgtc gaaaaaataa tataaataaa aaaatagatg aaaaaaatgt cgattcaaag
     6181 aacgttaata gtgatttctt agaggttaat acagataaag ttttatatga aaaactgtta
     6241 gtcattagag aagaatatgc aacaaaggaa aaattacctg catatatgat tttagccaat
     6301 aaggctttga aagaaataag tggaagatat ccacttgatg aagaacaatt aaaagatatt
     6361 tctggcatag gtggagtaaa atttgaaaaa tatggagcta gtattttggc agaggttaat
     6421 aaatatgtaa aagaaaataa tattaatgtt atttgggaga aaaagggcaa aaggaagtta
     6481 atattagatg gagaaagtag aaaaaataat gaaatagcat tagatttgct aaatcaagga
     6541 aaagatatac aaagtatcag tcaagaaata gaagtttcac tgtcaaccat tattggatat
     6601 gtgcatgact atgtaaagga tggacatagt atatcatttg aattaggact agaaaatttt
     6661 tataatgaag ataaaaagaa gatgatttta gaagtttgcg aaaaagttgg ctatgataat
     6721 ttaaataaca taaaaagaaa acttccagat tcaataaagt atgaaaatat aagagctgtg
     6781 atacttgact cttatcttga aaacttaaaa aataatatta tagcagagta atttttaact
     6841 taataaaaaa taaaatttgt attattgtgt tgaactaata attttgctgt gttatactga
     6901 atttgtaaaa ataatgaaaa attagagcta ggggcgctag aattggctag ctgagagata
     6961 aagccgtaac ttacaaaccc taataacctg atcaagataa tgcttgcgaa gggaagcttt
     7021 ttaatataat tttattatat aaataggcaa attattcaat gttaggagaa atttgtctaa
     7081 gtgtatatac gattataaga gtgctttcca aacgaggaaa agcacttttt ttattttcaa
     7141 aaactataac tttaatcttg gaggattgta atatgaacta tacaacacaa atggatgctg
     7201 ctagaaaagg cataataact aaagaaatgg aaatcgtttc tcaaaaagaa caggtagatg
     7261 taaatgaatt aagagaatta atagcaaatg gacaagttgt tataccagct aataaaaatc
     7321 ataaatcttt aagtgcagaa ggtgtaggta aaaatttaag aacaaaaata aatgtaaatc
     7381 taggaatttc aagagattgt aaggatatag aaaaagaact tgaaaaagtt agagtagcaa
     7441 tagacatgaa agctgaagct ataatggatt taagtaatta tggcaaaact agagaattta
     7501 gagaaaaggt tgttgaaatg tcacctgcaa tgataggttc agtacctatg tatgatgcag
     7561 taggatattt ggaaaaagaa ttaaaagata taacagaaga agagttttta aatgtaataa
     7621 gacaacatgc tattgatgga gtagacttta ttactataca tgcaggtctt acaagaagtg
     7681 tttgccaaaa aataaaaaat catgagagat tgactcatat agtatctcgt ggaggttctt
     7741 tattgtttgc atggatggaa ttaaataata aggaaaatcc tatttatact aattttgata
     7801 aaatattgga tatatgtgaa gaatatgatg ttacattgag tttaggagat gcttgtagac
     7861 caggttgtat aaaagattct actgatggtg ttcaaattca agaacttgta gtattaggtg
     7921 aacttactaa aagagcatgg gaaagaaatg tacaagtaat gatagaaggt cctggacaca
     7981 tggctattga tgaaatagaa gcaaatgtag tattagaaaa gagattatgc catggagcac
     8041 ctttttatgt tcttggacca ttggttacag atatagcacc aggttatgac catataacaa
     8101 gtgcaatagg aggagcttta gcatgtgcta aaggagtaga ttttttatgt tatgtaactc
     8161 cagcagaaca tttaagattg cctaatttag atgatatgaa agagggtata atagctgcaa
     8221 agatagctgc tcatgcagga gatatagcta aaaatgttaa aggtgctcgt gaatgggata
     8281 ataagatgag taaagctaga gctgatttag actggtgtga gatgtttaga cttgcaatag
     8341 accctgaaaa agctaagaga tacagagatg aatcaactcc tactcatgag gatagttgta
     8401 ctatgtgtgg aaaaatgtgc tcaatgagaa cagttaagaa aatattaaac aatgaggaac
     8461 ttaatctaat ataatttttg gctagtatga ctggaataaa aataagctga aaagaggagg
     8521 tgatttagtg aaggttaatg ggaaggaaat tgaatttgaa aaagatttga ctgttataga
     8581 tttattaaat aaatataatt taaaaagtga tagagttgta gttgaagtga atttagagat
     8641 aattgaagaa tctaactaca atacatatgt attaaaagac gaggacattg tagagcttat
     8701 tagctttatt ggaggtggtt aaaataaaaa tatttgtaaa tgaattatta acagatgttg
     8761 aggaaaatac aactgcttat aaagttagag atagctataa caataaatgt gatgtattgg
     8821 tgttaaatgg atatccaatt aaaacagata tgaccctttg tgaaaatgat aaattaacac
     8881 ttatacaaaa aggagttaag ccatcacttg atgaattgga aagcttgttg attgctagac
     8941 atactccaaa tatacataat aaattaaaaa aagggaaagt tgctatattg ggattggggg
     9001 gacttggttc caatatagct atctctttag ctagaatagg agtgggagaa cttttactta
     9061 tagattatga tgttgtagaa ccatctaatt taaatagaca gcagtatttt atagatgata
     9121 taggaaaact aaaaactgat gccatgatag aaaatataaa aaaaataaat ccatttataa
     9181 aattaaataa acgagatatt tttttaaaca agaacaatat ggatactatt aaggattcag
     9241 atttaataat tgaggccttt gatgatgctt catgtaaagc acaggtatgt aattatgtat
     9301 taataaattt aaaagataaa tatttaatag catcatctgg tatggcaggt tactatgatt
     9361 caaatataat aactactaag aagataaaag acaggtttta tatatgtggg gattttgtga
     9421 atgaagctaa atttggtgaa gggcttatgg ctccaagggt tgccatctgc gcaaatcata
     9481 tggcaaattt ggcaacgcaa atacttatag atatgtaaat attaaaatta gatataaaaa
     9541 agattgaata ttaaaaaaat ttaacgtgga gggaaataat atggataaat tggtacttgg
     9601 tggacatgaa tttaatagca gattattggt tggaacaggt aaatatggtt caaataatat
     9661 attgcctgag gtaataaaag aaagtggaag tgaaataata acaatggcac ttagaagagt
     9721 ggatttagac aataaacaag aaaacatatt aacgtatatt ccaaaagaga tgacaatact
     9781 tccaaataca tcaggagcta ctaatgcaga agaagctgta agaatagcta gaatatctag
     9841 aaaaatggga tgtggagatt ttataaaaat agaagtaata tcagatacta gatatttact
     9901 tcctgataat gaagaaacta taaaagctac aaagatactt gctgatgagg ggtttatagt
     9961 tcttccatat atgacaccag acctttatgc tggaagaaga cttatagaag ctaatgcagc
    10021 ggcagttatg ccacttggag ctccaattgg ttcaaataga ggtcttcaaa tgaaagaaat
    10081 gataagaata atgatagatg agttggatat accaataata gtagatgcag gtattggaaa
    10141 accatctcaa gctatggagg ctatggaaat gggagctgat gcagtacttg tcaacactgc
    10201 tatagcttca gcaggagacc ctgtacaaat ggctagagca tttaaattag ctgtagaagg
    10261 tggaagagaa gcatatattg caaaaacagg aaatgtgtca gaatttgcaa atgcatcttc
    10321 tcctttaaca ggttttttag gcaatttata gaaagtggtg attggtgatg agtttttatg
    10381 atgtaataga aaaatatagg gattttgatt ttgatggata tttaaataat gttactgata
    10441 atgatgtttt aagaagttta tcaaaagaca agcttgaaga ttttgatata ttaaatttgc
    10501 tttctaaaac tgcagtaaaa catttagaag atatggcaca aaaagcacat aaattaagtg
    10561 ttcaatactt tggtaaaaca gtatgtttat atacaccaat gtatatagca aattattgtg
    10621 ttaatcaatg tgtttattgt agctataata taaagagtgg aataaaaaga aaaaaattaa
    10681 ctatggatga aattagagaa gagggagaag caatttcaaa agaaggtttc aaacatctac
    10741 ttgttttgac tggagaaagt agttttcact caagtgtaga atatataggt gaggctatag
    10801 aaatattaag agagaaattt ccctctatag gtatagaagt ttatccaatg gaagtagaag
    10861 aatataagta cattgtagat aaaggtgttg aaggattaac agtatatcaa gaaacttatg
    10921 acgaggaaat atataagaga gtacatataa aaggcccaaa atcaaattat aaatttagac
    10981 tagatgctcc tgaaagaggg gcaaaagcag gtatgaggac tttatcaata ggtgcacttt
    11041 tagggctaaa tgattttaga aaagaaactt tttttacaat attgcatggg aaatacttaa
    11101 aaacgaaata tcctcatatt gagctttcat attcaacacc aagaatgaga ccttttaaag
    11161 ggtgttttga ggaattggta gatataagtg atactgattt agttcaagct atggtatgta
    11221 tgagattatt tgaccctcat gcagctataa atatatccac tagagaaaat ttagaaatga
    11281 gaagtcatat aataccttta ggagtaacta aattgagtgc aggtgtatct actgatgtag
    11341 gaggtcattc tcaagatgaa catgatactg ctcaatttaa gataaatgat gaaagtactg
    11401 ttaaagatgt ggagaaaatg ctaaactcaa ttggttatca gcatgtgttt aaagattggg
    11461 aaagatttta aatgtatctg ataacaaaca gaaaattatg tagtgaggaa cgatatttag
    11521 aagttattaa agagtctata ttgagtggtg tagaaaatat aataataaga gaaaaggatt
    11581 tagaatatca agagctaaga aaattatata tgaaaataaa aactaaaatc aattgtatag
    11641 attttcaaga gcaaatatca gatgaaagtt tgaaaactaa tataaatcaa aaagagtgta
    11701 gaaataaatt caaggttaat tttataataa atagtaacat tgaatttttt gaaaaagtgg
    11761 attgtcaagg tatccatctt ccttttaaat tattcttgaa tttaattgaa aataagtata
    11821 attttaatga aaataaaata ctaggattat ctttacataa agtagaggaa gtagattatt
    11881 tagaaaaatt aataagaaat caaaatataa aaatagatta cataacatta tcacatatat
    11941 atgaaactaa atgtaaagaa ggattaaatc caaaaggaat tgagctttta aaagaagcta
    12001 aaaagattac tgatattaaa ataattgcac ttgggggaat tttaccatct aatgtcaagg
    12061 aaacattaaa atactgtgat gattttgcta taatgtcaac tataatgagg tctaaagata
    12121 ttaaaaaaac catatcaaat tataatgaaa agttgaatta gtaaatatag tatattgaat
    12181 ttttaactta aataaaaata aaaaagttat cttttaaatt ttaaaaggta gcttttttat
    12241 tttatatctt tgctaataaa aaattgtaaa aagggaaaac tttagtcaga gagattaaac
    12301 aatatcctag aaagatttaa aatttagtaa agtatggagg acattataat gactgacagt
    12361 aaaaagaaac gatttattat accacacact tttacaataa tatttttact aatagtcttg
    12421 atggcaatat tgacttggat agtaccaagt ggtgaattta gtagagaaga tgtagaaggt
    12481 agaatggtag ttgtaccagg gacttatcaa agtgtagaaa gtaatcctca aggatttgca
    12541 gatgttttta aagcaccaat agaaggcttt atcgattcag ctgaagttgt aggttttgtt
    12601 ttaatagttg gaggtgcttt tggtattgta aataggactg gagctataga agcaggaata
    12661 gcttcggtta tctctaagtt taagggaaaa gaacttctta taattccaat atcaatgatt
    12721 ttatttggac ttggaggtac aacatttggg atggcagaag agacattacc attttatatg
    12781 atatttgttc cactcatgac atccttggga tatgattctc taacagcaat agcaattgta
    12841 tttataggag cagcagcagg agccgccgct tcaacaataa atccattttc agttggtatt
    12901 gcacaggcac tagctcaact tgctcctggt tcaggagtaa cttacagagt agttattttt
    12961 atagcatata tactaataag tatagcattt gttatgtact atgctaagaa ggtaaaggaa
    13021 aatcctgaaa gttcaatagt atatgaaata gataaaaata ataaatcaat acttaatcaa
    13081 acttcaacag aaataaaaga atttactttt aaagaagtat ctgttcttgc tatatttgga
    13141 atagggatgg caataatgat ttggggtgtt ttaagtcaag gatggtacat tccagaaata
    13201 tcaatgtgtt ttgctacaat aggtgtgctt tctggattgg ttggtagatt atctcaaatt
    13261 gaaatctcag aaagttttat agatggagca aaagatttag catctgcagc tcttgctata
    13321 gctttggctc gtggaatcgt aataattgct caaaatggat ttataataga tactatatta
    13381 aattccgcag cagcctttct aggtgggctt ccaaaggtta tatttgtata tttatcattt
    13441 ttcttggaag ctgcattggc atttttaatt ccatcatctt caggtcttgc ttcacttaca
    13501 gtacctgtac ttgctcctct tggtgatttg gttaatatat cggctcaagt gatagtaacc
    13561 gcttatcaat ttggtgtggg tcttacaaac tttataactc caacttcagg agtactgatg
    13621 ggagcacttg cagtagccca tattccattt tcaaaatggg taagatttat aatgcctatc
    13681 ttgttgattt tgacagctgt atctctagtc tttttaacaa taggtattta tatagggttt
    13741 taattaagtg tagttttatt taagatattt agtaatggta taaaatgata atatgttttc
    13801 aataaattgc cactaattta attagtggta atttgttttt tgtatttaaa aataaatata
    13861 tttacttgta ttttgaatta atatctactt agacattgtt ttaattgtta tattttcatt
    13921 taattaagca ttgatttatc attttttata tttcatataa caatgcaaaa aataattttt
    13981 atatagtaaa tgacaaaagt ttacaataaa tgtatatttt cttactcatt ccttaaaatt
    14041 actcatttag tttaataact ctcatttcat atgtaaagtt ggtaaaaaaa ttaaaattaa
    14101 aagtaaaaaa ctaaataaat taatgttaat ttgaaatata tatgaaggtt tttggagaat
    14161 acttacttat gataggaaaa aatgttaaaa taatagtgta atcaaatata aaggactgaa
    14221 ataattaaca gttattaaat attaggagtg taaaaattta tgggattaaa agaatttgat
    14281 tttattgaag agtctattga tatgttgagg acaatgtcac caacattaga aactatatca
    14341 gatgaaatag aagagtattt tgaaaatatc ttggatgaaa agaatcaaga atatataaat
    14401 gtaacctcta gaataaaatc agaatcaagt ttaagagaaa agataattag aaatagatat
    14461 ttaaaaaaat atggagaagc aagtaatctt attcataatg tttctgactt aataggccta
    14521 agaattgagt gtagatttat tgaggatgaa aacaaaatat atagactttt gagaagatat
    14581 tttaataaaa cagatgacaa aataaactat tataataaag agaataagaa tattaaatta
    14641 aaactatctg agagacaacc caataagcaa aaaaatggat ttgaaatata taaaatagat
    14701 ggagtttttg cgtatttaga tagagaagta aaatttgaac ttcaaattaa atctcttgta
    14761 aatgtgtttt ggagtgaaat agaacataaa ataatttata aaaacaatac atatttactt
    14821 gctgataaat ttataaagga tatgatggac tctataaaga ataatcttac tatgatagat
    14881 aatcagcttt taagtattta taaaaacttt cattcaggga agtcttttaa tatgaaagta
    14941 agtaaaaaag aaattgaaaa attatttgcg aaactagtct atgacgcttt ttctgaaaag
    15001 atgaataaaa gtataggttt tgttgtagat tttaaaaaac catgtgagac tatattgaac
    15061 tattctttta ataaacaaga tataagtgat gaagttttag gtaattttat gcttgatgag
    15121 tttgcaagat taaatgaaat tgtaaacaag gatatagatt ttaatgagca aatagaattt
    15181 gaaagagaac caacatttga tgataagttt tgtaaagacc taggaaatca ttttagaagt
    15241 agattaaaca cagaatttcc atggaaccta ttttttagaa tattatttga aatagagcca
    15301 tataacaata ccaaagactt tgaaaacttt gtagagttta taaaagaaaa tgtgctacta
    15361 gaagaaaata gggagcaatt attatgccaa tttgaagaag atagcaaact tataattgaa
    15421 gatatatatg attgtatatt taatagtata agtgagattg atagtgttga aattttatat
    15481 aactataatc ttgaaaaaat aaactttaca tcaagtgaaa tcataaattg tatatgtaga
    15541 gaatatgagt gttatggtga atacttagaa gaaaaagaaa agcttatgaa tacatttaaa
    15601 gaaaagatta ttcaaatctt tgagtaatag gtcaatgctg aagtggcgct aatgcgccat
    15661 ttttcaaata tatatgaatt aagtaaaatt attttggagg aggcgatttg tatagagaaa
    15721 acaggtgtaa ttttggcagg gggaagaaat tctagaatgg gtagagataa agccttttta
    15781 gagttacatg ataagctttt tattgaaatt gcaatagaag catttaaaaa ctttgatgag
    15841 ttgataataa tatctaataa tgaagaatta tattctaaat acgatattaa agtttataat
    15901 gatatagtaa aagatgttgg tccaattgga ggaatatata ctgctcttga atatgcaaag
    15961 tatgatatag ttacaattgc atgtgatatg ccatatctaa ataatcaaat agtcgaaaga
    16021 atagctaata aaatgaatga taaaagtgtg atttcagtga caaatggaaa gttacaacca
    16081 ttgtgttctg gatataaaaa gagtataata aataaagtat ctttatgtat aagggaaaat
    16141 gatttaaagc tcagaagctt tatagataaa atagataaaa gttatatata ctttgatgaa
    16201 gaagatttat ttttaaatgt aaacactgtt gatgagtatg aaaaattaac taaagtttaa
    16261 ttagctatgg ttgtaaaata gagtaggatt tatatggagg agagtttatg aatttactta
    16321 aatgcgattc ttgggcagta atattaaata atagcgataa agaaagtaaa gcatataaaa
    16381 tattagatga acttaaaagg aatatgtaca aagttgttgc tatagatgaa gagaaaaaac
    16441 ctattgaagg aatagatgtg tatgaatgtt taaaagatgt tccacacaat attgatgttg
    16501 tagctattat tgataaacaa tctaagatgg atgtgatttt agaagaagta gagcttttgg
    16561 atatacaaaa tatgtggttt gaaaaaggaa gctttagtga aatgatgatt aaaaagacta
    16621 aagatttaaa attaaatata gaatataatt taagtttata tgatgagttg actagataaa
    16681 attatatatc ttaataataa aaatataatt agttaataaa acatattatt ttatagtata
    16741 gattggttaa taaaagtaca gattggttaa tgaaacatta aattacaata tttgttaaaa
    16801 aaattgtgag ttacatgata taatgtatta aataggatac taaggagttg atatattatg
    16861 ttaatagtaa ctacagaaaa agtagaaggt aaaaagatat caaaggtttt aggattagtg
    16921 agaggaagta caataagagc aaaacatgtt ggaaaagata taggagcaag ttttaagaat
    16981 cttgttggag gagaacttac tggatataat gaaatgctca ctgaagcaag acaaattgcc
    17041 ataggtagaa tggttgaaga tgcagaagct aaaggtgcaa acgcagtaat agcatttaga
    17101 ctgtcttcag cttcagttat gcaaggggca gcagaaatgc ttgcttatgg aacagcagtt
    17161 gttttagaag atgataatag tattcttgaa aaataaaata taaatagtta aaaagaggca
    17221 tcttttaaaa attttatgga tgcctttttt tatttgaaaa aactaatttg agaagaaatt
    17281 ttaactatta tgacaataaa attagggtga tattatataa ataagtaaaa ttatattata
    17341 taatgtatgg tatagacttt aaaaatttta tttgtagaga aaaatatgga caatgttata
    17401 ataattatag aaattaaaag tacttacatt cattatgtat gtgacatgta taattagttt
    17461 tatatttaat acagttttga agtaatatct tcattttgag aacgttggaa taataaaaaa
    17521 tgtattatta taggtgatat aaataaaaaa taaatataat atgttatatt gctgatagaa
    17581 aagagaataa aagaaaatag gttttattaa aagagatttt tatttatagg acttaatcaa
    17641 tgtatagagg ggtgagaaca tatgtttaat gttgcaatta taacacttag tgataaagga
    17701 tatgaaggaa aaagagaaga tataacagga aaaaagttaa ctgaatttgt tgaaaataca
    17761 ggagcttata aagttactga atatgtactt ataaaagatg ataaggaaat gctaaaggaa
    17821 aatattataa gactttgtaa tagtaataaa atagatttaa ttcttacaaa tggaggtact
    17881 ggtttttcaa aaagagatat aaccccagaa gcaactaagg aagtgttgga aaaagaaata
    17941 ccaggtctga gcgaatatat gagaatgaaa tccacagaaa ttactaaaaa ggccatacta
    18001 agtaggggtg taagtggaat aagaaataat tctataataa taaatttacc tggaagtcct
    18061 aaaggtgcag ttgaaaatct aagttttata attgatgttt tagaccatgg catagaagtt
    18121 ttaagaggag aagctacaga atgtgccact aaaaaaagtg aatagtatat aatctagata
    18181 gataaaaaat agacaaagag gtagataaag tatgattgat aagtatggaa gaaaaataga
    18241 ctaccttcgt atttcactta cagataagtg taatttaagg tgtgtttatt gtatgccacc
    18301 agatgtaaaa tttgataaaa attatataaa tgaaaatcta agctttgaag actacaagtt
    18361 tattataaag gctatggctg aacaaggtat aacaaaaatt agatttactg gaggagaacc
    18421 tcttttatat cctaaattaa atgagcttat aaaatttaca aaagaagaat gtggaataaa
    18481 taacatagga atgaccacta atgctattgg gctttctgat agaatctttg agcttgaaaa
    18541 atgtggctta aataaagtta atataagttt agattctttg aaggaatata agtataaatc
    18601 cgtaacaaga ggaggaaact taaaagatgt cttaaaatcg attgaatctt gtttaaactt
    18661 aggtataaaa gtaaagttaa attgcgttgc tataagtggg tttaatgatg atgagcttaa
    18721 agattttatg tttttaacag ttaaatcacc aattgatgta agatttatag agcttatgcc
    18781 attaggtgag gctaatagag tttataagaa gggttatttt aatgtaaaag aaatgattga
    18841 aaaaatatat ggtttatata aagtcaaaaa tgatgaaaaa agtacagcag aatattatat
    18901 gtttaaagga gctaaaggaa gaataggaat tataactcca ttaagctgtt cattttgtaa
    18961 cacttgtaat cgtattagac ttacttctag tggtgcaata aaattatgtc tacattcaaa
    19021 agaagaaata gatataaaac catttttaca taaaccattg ttatttagag aatctatgaa
    19081 agatataata aaacaaaaac cagaaaaaca tcatttaata gaaaataaat gtagtgatac
    19141 aaataggcgt atgtatgaaa ttggaggata agtaatgtta acacatataa atgaaaaagg
    19201 gtatgccaag atggtggaca taagtgaaaa agatgatact aaaagaacgg ccgtagctac
    19261 agctacaatt actcttagta aggaagcatt agaaaaagta aaaaatggtc agataaaaaa
    19321 aggagacgta ttgtctgtag ctcaagttgc aggtataatg ggagctaaaa atacatcttc
    19381 aaacatacca atgtgccatc agattaaatt agttggatgt gatttagaat ttgatgtaga
    19441 tgattctatg tctgctatta agatatacgc aacctgtaaa tctcttggga aaactggagt
    19501 agaaatggaa gctttaaatg catgttcttt agcagcactt acaatttatg atatgtgtaa
    19561 agcagttgat aaaaaaatgg ttataagtaa tatccatctt gtagagaaga ctggtggaaa
    19621 atcaggagat tttaagttcg agtaaattaa taatatattg ggttaaaatt tggaggaaat
    19681 atggcgaagg tagtgtcaat aaatataagt gagaaaaaag gtgtaattaa agttcctgta
    19741 caatgtgcag agctaaaaat aaatcatggg atagttggag atgcacatgc aggaaactgg
    19801 catcgtcaga taagcttact tggaaaagaa agcatagata aaatgaaaat gaaaggcttt
    19861 gaccaattaa aatatggaga ttttgctgaa aatgtaacta cagaagggat agaagttttt
    19921 tccctgcctg ttggtacaaa gttaagaata gggcaatgtg aagttgaagt aactcaaata
    19981 gggaaaaaat gtcacaatgg atgcgaaatt aagaaattga caggtgactg tgtgatgcca
    20041 cgagaaggaa tttttgtaaa ggttctaaaa gaaggaatta taaatgtgaa tgattcaata
    20101 gatgtaatct aatgaagtca ctattaaaat ataat
//
LOCUS       DAMJVI010000071        19933 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.073, whole genome shotgun sequence.
ACCESSION   DAMJVI010000071 DAMJVI010000000
VERSION     DAMJVI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.073"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            256..1761
                     /locus_tag="PC545_002527"
     CDS             256..1761
                     /locus_tag="PC545_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2457291.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELIVSNNV
                     DDVGESEVIDINPNNYLKKTLINYSESTLNLYAAVESIRGQFIDPFKIPLIIYDDSWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSSNQFVCEYGITIYNIPILYKSNSIGV
                     IRGGYVLLSDLNLDTEHNNLYDIPEGAARSIKRLLKQISKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMASIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIKNYLNLQKLRYGDALKVKYLIPEKLYNLSVP
                     FNFIQPIVENAFTHGFRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDGNNIDKIKAG
                     IRSNNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPIENYKEYV"
     gene            1875..2573
                     /locus_tag="PC545_002528"
     CDS             1875..2573
                     /locus_tag="PC545_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2457292.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     IGLEPDIVFIDIQMPIYNGLEVIEKTSHLSKKINFVVITAFNYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIE
                     KGMTPKVYKLNTKN"
     gene            2858..3946
                     /locus_tag="PC545_002529"
     CDS             2858..3946
                     /locus_tag="PC545_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457293.1"
                     /translation="MSDKVNMKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     IGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIVSGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNISPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCRKKSTQSQE"
     gene            4185..5375
                     /locus_tag="PC545_002530"
     CDS             4185..5375
                     /locus_tag="PC545_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457294.1"
                     /translation="MHELILISEKYKEDILDFFTDIHMHPELSFKEFRTTKAIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPIYEEVDIKYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEN
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYAIACKSVCDLYSEDAIVDPIPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCIYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KNKI"
     gene            5466..6968
                     /locus_tag="PC545_002531"
     CDS             5466..6968
                     /locus_tag="PC545_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HDF2457295.1"
                     /translation="MRALNVVERAGNKLPDPVTIFLLLCIIVVILSAVISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDPSYQGNPAMNLYFAFISAIVLTFAGAF
                     VTEKFIAPRFTKYNDSTNDKDIQELSELENKGIKYALLSLLVVVVVIVALCIGDNAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGKVSKKIKSDKDLVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKLLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEIIILLLFMGIGIPLGPGGGISYVL"
     gene            7040..8296
                     /locus_tag="PC545_002532"
     CDS             7040..8296
                     /locus_tag="PC545_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDF2457296.1"
                     /translation="MLKTTKISDPELYKIVADELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSAVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECRPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIVLCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLST
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            8468..11242
                     /locus_tag="PC545_002533"
     CDS             8468..11242
                     /locus_tag="PC545_002533"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2457297.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAHALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITPKVEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAMTSEGELNLIGNSRSYMKEDNLVFSGFVGIVDPLREGVK
                     DSIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFTQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRTMKN
                     SRTMNFNSKN"
     gene            complement(11343..11492)
                     /locus_tag="PC545_002534"
     CDS             complement(11343..11492)
                     /locus_tag="PC545_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDF2457298.1"
                     /translation="MFFISYIYTKSPIEFDTPLQKEAYKILQKLDIDFECVDTDEAIT
                     MEDCV"
     gene            11867..13045
                     /locus_tag="PC545_002535"
     CDS             11867..13045
                     /locus_tag="PC545_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2457299.1"
                     /translation="MFSKILVKTFIRDSENVQNTDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPIPVKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEEMVNSIIELLLSYKYIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPICLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYNCVIVVDKDFI
                     "
     regulatory      13172..13257
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            13375..16293
                     /locus_tag="PC545_002536"
     CDS             13375..16293
                     /locus_tag="PC545_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HDF2457300.1"
                     /translation="MKKGNRKALLISLIMILSMVVSTIYPTVSYASELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEKDKATDNKKPEQTPEEEKPTDNKKPEQTPEEDKSTDN
                     KKPEQTPEGEKPIDNKKPEQIPEEDKSTDNKKSEQALEDEKPLDNKNTEKTPEEDNLL
                     EDENLLKVLEEELNEENEDYGFVVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSI
                     TFKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSVMQGKIGLEAYVKKVDE
                     DTNAKIHIETTGKIESGSGDIDVEIKPGDKTDVPDAKGTLKKLVEGKKSTTVFMPVKN
                     KDINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNIEQSKNSI
                     SVNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSIFDRGDD
                     YLLTKGHSGATNITQVGQVINYQISINDDKSPISNVVITDNIPEGMRLTTSGEAGHDF
                     RVVEIPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFF
                     IAYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLNSSDN
                     QIVKYKINMSTYGVYDAGQVNLLDEVNSALEISNIKYSDNLELKKEAGDGKNTIRLVN
                     KYEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNVAQINGSSPPGVETTKQGYAFEAK
                     KVDALDKNVLSGAKFNLEDAFGNIVVKDLVSDEDGIIQSSIKKPGTYYLVEIMAPSGY
                     EKLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVNPPAPPNTDEPIVNPPVPPSTDK
                     PRKPSSSSDTEDTIVINPPIPPSEDIINPPIPEVLNPPVPPSEEMIETPVKQIIPIPE
                     VVKPSVSEEKNNKVKDDTLVNPPVPPKTGDSTTIIGEILLVIGAIVGLIVLRRNKNTN
                     "
     regulatory      16479..16564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            16664..17326
                     /gene="zmp1"
                     /locus_tag="PC545_002537"
     CDS             16664..17326
                     /gene="zmp1"
                     /locus_tag="PC545_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HDF2457301.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHADSTTIQQNKDTLSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNALKQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYAEEFFAESFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     gene            complement(17448..18410)
                     /locus_tag="PC545_002538"
     CDS             complement(17448..18410)
                     /locus_tag="PC545_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2457302.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALSLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILNKNKLHVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMVIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(18580..19770)
                     /locus_tag="PC545_002539"
     CDS             complement(18580..19770)
                     /locus_tag="PC545_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2457303.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTRENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALKNAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTSIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQMDDFDDVMSSLNVA
                     NRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLIDMGYSCVKPNGTFYLF
                     PKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYDKVKSSLPAFEKLAKL
                     YNLK"
BASE COUNT         7839 a         2329 c         3391 g         6374 t
ORIGIN      
        1 tgatacaata atatagaagt aataaaaaat agttaatgta aaagtgatac aataatatag
       61 aagtaataaa aaaatagtta atataaaagc gatacaataa taatagaagt aataaaaaaa
      121 aataattaat ataaaaatgt gtacaaacta atgaatattg atatagtaag ctatcatatg
      181 aaagagtatg tacacatttt ttttactaat ttttcacaat atgaacataa ataatttaca
      241 ggaaaggaat cttttatgaa aattcaaaat actgagtggg gatatattga atggaaacat
      301 acttatgatg aaaataatcc aaaacaagct atgaatatat acattgcagt aactatgcct
      361 ggtaagaaac attttaatca tgttcattat ggtcaagagc agatgatata tattttagag
      421 ggagagggtt tgtatattat aaatggagtt tggaagccat tttatcaagg tatgattttt
      481 tatatagaat ctggtagcac acatgaaact ataaatacag gcgatagaga aataaaagaa
      541 cttattgttt caaataatgt agatgatgta ggagagagtg aagttataga tattaatcct
      601 aataattatt taaagaagac cttgataaat tatagtgaaa gtacattaaa tttgtatgca
      661 gcagtagaat caattcgtgg tcaatttata gacccgttta aaattccatt aattatatat
      721 gatgattcat ggaatatagt tttaaaaaat ccttattttc cacttttttg ttttgaaaaa
      781 tgtaatccta tgaaatttcc tcaaaattgt gattgtatga atcaaaaaag tagcaatcaa
      841 tttgtatgtg aatatgggat aactatatat aatataccaa tactgtacaa aagcaatagc
      901 ataggtgtta taagaggagg gtatgtacta ttatctgatt tgaatttaga tacagagcat
      961 aataatttat atgatattcc agagggagct gctagaagta taaaacgcct cttgaaacaa
     1021 atatctaaaa atataataaa tttttgctct tttaatgata taagaaagga tttacaagaa
     1081 aaagagaaga ctatagcaag aacatatcat tatggagagc aacttgaaat gaatttaaaa
     1141 gtagctcaag atatggttac taatttaaga ataaatcatc actttttatt taatacatta
     1201 aattcaatgg caagcatagc actagatgat ggtagttatg atttgtatag tgcaataata
     1261 gatttatcaa gaatgtttag atatacaatg aggtctgatt taagatttgt agaattagaa
     1321 tctgaaatat tatacattaa aaattatttg aatttacaga aattacgtta tggagatgca
     1381 cttaaagtaa aatatcttat tccagagaag ttatataacc tttctgtgcc ttttaacttt
     1441 attcaaccta ttgtagagaa tgcatttaca catggattta gagatattga cacagaaaag
     1501 cgaattgaaa taattgcaag attagattct cagtatgcca ttatagagat tcataataat
     1561 ggaactatct tagatggaaa taatatagac aaaataaagg caggtattcg tagtaataat
     1621 ggtcatggtt tgtctttaat atatacaaaa ttcacatctg cttatggaaa taattttgat
     1681 atggatataa agtcgagtga taatgaagga acttacataa tgattaaaat tcctattgaa
     1741 aattataagg agtatgtata gaacttgtga aaaattaata agttttataa atgttaagaa
     1801 atatattaga tgtttttatg ttgttttata tatcttactt ttttatatta ttaagctaaa
     1861 agggaggtat tctcatgaga agagcactaa taatagatga tgaagaagct gttctaaaaa
     1921 taataacaca ttttatagaa atgaaagata tgccaataaa aattgttggt aaagcaacat
     1981 ctggtgatga agcagtagat aaaatcattg gtttagagcc agacatagtt tttatagata
     2041 ttcaaatgcc aatatacaac gggttagaag taatagaaaa aacatctcat ttatctaaaa
     2101 aaataaactt tgtagtaata actgcattta actattttga atatgctcaa aaagctttga
     2161 gattaaatgt aaaagatata ctccttaaac cattggatat gaaagaattt actaagtctg
     2221 tagaaaaaat tattggatat caatatacca ataatgattt attaaatgag atactggaat
     2281 atataaatct aaactatagc aatgacttaa agttaaatga ttgtgctaga ttatttttaa
     2341 ccaatccatc taatatatct aggattttta aaagtaattt aaatacaact tttatatcat
     2401 atctaaatca tataagaata gaaaaatcta ttgagttact tgaaaatact acaaaaagta
     2461 taaatgagat tgcagaaata gttggatata atagcctaaa taacttttat aaaaacttta
     2521 agatagaaaa aggaatgact ccaaaagttt ataaactgaa cactaaaaat tgaaattgat
     2581 aaataaaaag tcaatataga taattgtttg agtgtgtctg aataagtata ctttaattat
     2641 gataaaaatt aaagcgcttt aaacatattt aataatatta aaccttataa aagctgtacc
     2701 tatttatata aaaaaatata tataaataaa aagaaaaaga tatacaaaat aaaaaaagtg
     2761 aattaaatac tgtttcaaaa agacagaatt ttcatttaat ttactgagag tttatatttc
     2821 ttaaaggaag gtaactttat aatggaggta atttaatatg tcagacaaag tgaatatgaa
     2881 aaatattatc tgtattgcag gcgcttatct agcaacagca attggttctg ggtttgctac
     2941 tggtcaagaa attctacaat tttttgcctc acaagggtat atgggtttaa ttggagcatt
     3001 aatatcaatg gtattgtttt catttcttgg tgctgaagtt ataactagag gtagagaatt
     3061 aaaacttaaa gaaccaacaa gaatttacac tttttattgt gggaagattt taggaaaatt
     3121 ttacgaatgg tttagtccaa tatttttatt tgggattctt atagtaatga tttcaggtgc
     3181 aggctcaact ttaactgaat actatggtat aaatccttac ttaggaagag tattgatggc
     3241 tgttgctact ttaattacag tgtctatggg tttagaaaaa ttagtaagca tacttggacg
     3301 tattggacca gtgatagcag tatttacaat tgctgttgga ttaataagct tgtttaagca
     3361 ttttgacggt ttatctatgg ttggagaagc tatgaaaaat attgaagtta caaaagcatc
     3421 agaaagtcca atcgtttctg gtgtaatata taatacatta aatattattg taatgattgt
     3481 ttttttaaca ggaatgggag cttctattga aagtaaaaag gatgcttttt ggggaggttt
     3541 attaggtgga atagtgttta tgactgctgg tatagttatg tatttggcta tgatttcaga
     3601 tataggaaat ttgtatatga aagaaatacc atcactttat ttagcagata atatatctcc
     3661 gattgtaggt gtatgttttt cagttgcatt aatattaggt atctatacaa cagctgttcc
     3721 acttttatgg tctgtaacca atagaattgt tgaagatgaa catcctaaat ttaaattggt
     3781 aacaacagta atagctattt tggcttgtat aggaggtttt ttaccgtttg gtaaattagt
     3841 aaacatttta tatccatata caggttatat ggggctttta attttagtct gtatggtgta
     3901 taaagcaatt acatgtagaa aaaaatcaac acaaagtcag gaatagagcc acttattaat
     3961 agtttttatt taaaaaaatt ttttatataa tccgaattta aaaagtcaaa atagttaaat
     4021 aaattgcaaa acatgataat atatttaatt aaaataatat agtaaaatat aaatataaac
     4081 taaaattaaa tttataaaaa atatctttat aaagattatt aacttatata atttgtagtt
     4141 aaattataaa aaataaaaaa ataaaatttt ggagaggtgt tagtatgcat gaacttattt
     4201 taatctcaga aaagtataaa gaagatattt tagatttttt tacagatatt catatgcacc
     4261 ccgaattaag ttttaaagaa tttaggacta caaaagctat aaaagattta ttagtgagtt
     4321 tagacataga aatattagat ttaggaatgg aaacaggcgt tgttggatta ttaaaaggga
     4381 agtatgatgg tcccacagta gcactaagag gagatattga tgcgttgcca atatatgaag
     4441 aggttgatat taagtataaa tccagatatg atggtataat gcatgcatgt gggcatgata
     4501 tacacacgtc ttgccttgta ggatgtgcat atgtattgtc tcatattaga gatagtcttc
     4561 atggaaatgt aaagtttata tttcaaccag ccgaagaagt caataaaggt gcgaagatgt
     4621 tagtggaacg aggagtaatg gaaaatccaa aagttgatgc tatatttgga cttcataatc
     4681 atccagatat accttgtgga aaaataggtg ttaagttagg tgggttaatg gcagcagtag
     4741 acactattaa gatagaagta aatggatttg gaggtcacgg aggtatacca aacagaacaa
     4801 tagaccctat agtagcatca agtgcaatta taatgggtat tcaaaccata gtaagcagaa
     4861 acataagtcc attagaatca gctgttatat caataggtac tataaatgga gggactgcaa
     4921 ataatgtaat ttcagaaaag gtagatatga caggcacatg tagaagcttt agtaatgaag
     4981 tacgaaagaa aatatcagaa aatttagaaa atatagtttg tgagatagct cgtggatatc
     5041 aagcaactgc aaagctagat tatttatttg atttaccagc tgttataaat agtaaagaga
     5101 tgtacgcaat agcttgtaag tctgtgtgtg atttgtattc agaagatgct atagttgatc
     5161 caataccttc tacaggaggg gaagattttt ctatattcat ggaaaaagcc ccaggatttt
     5221 tttactggtt aggggtaggc aacaaagaac aagattgtat atatcagtgg cataatccta
     5281 aatttaaagc tgataaaaat tctatattgg taggtacaaa tgtactatgt cagtctgtta
     5341 taaattatat ggataaatta aaaaataaaa tttaagatag agcattctgg agataaagtt
     5401 tatgaggggg tattagtttg aggaaaatta attcacagga aaaaaacaaa gatagtggat
     5461 tattgatgag ggcactaaat gttgtagaga gagcaggaaa taaacttcca gacccagtaa
     5521 ccatattttt attactatgt ataattgttg taatattgtc agcagttata tctaatttag
     5581 gagtagaaga aatacatcct tctacaaaag aagttgtaaa ggtagtaaat ctactagaga
     5641 aagagcagat tcaatcatat ttaggtagca tagttacaaa ctttcagtca tttgctcctt
     5701 taggattagt actggttaca atgcttggag caggtgttgc tgagaaatct ggatttatgg
     5761 aagtgttaat gaaaaagggt ataagtaaag ttccacaaaa acttgtaact gtagcaatcg
     5821 tatttgcagg tatgttgtca catacagcag cagatgtagg atttataata ttaccaccac
     5881 ttgcagcact tgtgttttta ggaattggaa gacatcctct tgtaggtatg tttgcagctt
     5941 ttgcaggtgt tgcaggggga tttgcagcaa atgttatgct tagtactaca gatgtattgt
     6001 tagctggatt tacaatacca gcagctcaaa tgatggaccc atcttaccaa ggcaatccag
     6061 caatgaatct ttattttgct tttatatcag cgatagtttt aacttttgca ggagcatttg
     6121 taactgaaaa atttatagct cctcgtttta caaaatataa tgattcaaca aatgataagg
     6181 atatacagga attgagtgaa cttgaaaata agggtataaa atacgcttta ttatcattgt
     6241 tagttgtagt agtagtgata gttgccttat gtataggtga caatgcattt atgaaagatc
     6301 cagagactgg ttctatacta tctagtaatg cacctttaat gaaaggtata gttcctataa
     6361 tcactattat atttttaact ccaggattag tgtatggaaa agtaagtaag aagataaaaa
     6421 gtgacaaaga tttagtatct atgatgggta gtagtatgag tgatatgggt ggatatatag
     6481 tcctagcatt tatagcatcc cagtttatta atttatttaa tcttagtaac ttaggaacaa
     6541 ttttatcaat aacaggagca aaattactag cagagtcggg aatacctagc tatggattga
     6601 taataggatt tatattgcta tctggattta tcaatttatt tgtaggaagt gcttctgcaa
     6661 aatgggcgat tttagcacct atatttgttc ccatgtttat gctactagac tttaatccag
     6721 cattaactca aatagcttat cgtattggag atgcttcaac aaatccaata agtccacttt
     6781 ttccgtattt tccagttata ttggcctttg ccagaagata tgataaagat ataggtatag
     6841 gtactgtcat atcgaatatg attccatact cagtggtatt tacattaata gaaataataa
     6901 tattgttact attcatgggt attggcatac cattaggacc tggtggagga atatcttacg
     6961 ttttatagac cgttataata aatttatcaa tgaagattaa actaataaaa tatattcttt
     7021 aattaagagg aggattatta tgttaaaaac aacaaaaatt tcagacccag agttatacaa
     7081 gattgtagct gatgagttgg ttagacaaga acacaatatt gagatgatag cttctgaaag
     7141 tacagcacca acagaagtac ttgaattaag tggatgtgta tttacaaata aaacagaaga
     7201 aggctatcca ggggctcgtt ttcaagcagg ttctgaagaa gcagataaac ttgaaaccct
     7261 agcaataaaa cgtgcaaaag aagtatttgg agcagaacat gtaaacgtac aaccgtattc
     7321 aggttctaca gctaactatt gtgtatattc atcaatatta aaaccaaatg acactgtttt
     7381 aagtatgcgt cttgaccaag gtggacactt gacacatggt agcgcagtta attttttaca
     7441 tgacatttat aaatatgaat tttatggggt agacccaaat actggacgta tagattatga
     7501 tgctcttgaa gcaaaggcaa aagaatgtag accaaaactt ataattgcag gggcaagttc
     7561 atatccaaga ttaatagatt atgaaagaat atcaaaggtt gcaaaagaag taggagctta
     7621 ctttatggta gatatggcac atgttgcagg attagtcgca gcgaaagtaa tacctagtcc
     7681 tgtaccttat gcagattttg tttcttcatc tacgacaaag actttctgtg gaccaagaag
     7741 tggtattgta ctttgtaaag cagaacatgc aaagaaactc gataaaggtg tttttccagg
     7801 aacattaggg tctatacatc ttaatacagt tgcagcaaaa gccttttcac tattatattt
     7861 aagtacagat aaatttaaaa agataatgga gcaagttgta gtaaatgctc agactctagc
     7921 ttctgaactg atttcacatg gttttagtat tgtaagtgga ggtactgata atcatatagt
     7981 aatggttgac ttaagaagta agaatcttac aggaaaacaa tttgaaaaag ctcttgaata
     8041 tgtaggaata acagttaata aaaatgttat acctgacgac ccacaatcac catttgtaac
     8101 tagtggtgtt cgtataggtc ttacatcaat atctcaaaga ggtctgaaag aaaaagaagt
     8161 tatacagata gcagggataa tgaataaggt agcagaaaat attgataata aagaagtatt
     8221 agatgaatgt aaagcagagg cacaagaact tatatctaag tttccattat atccagaagg
     8281 gtattttgaa gattaaatat tttgatttgt atagctcaca atttataaag cctgtaacat
     8341 aaaatttaat aatatataac cactataagg tgttgattaa tcaacacctt attttttttg
     8401 aaaagtaata tacaagctat cataagcata taattcctta tagataatta acaaggggga
     8461 taaagttatg aggtattata ataaacctac caaagaagta ttaaaatact taaaaacaaa
     8521 tccagaaatt ggacttgatg ataatgaggt agaagaaagg aagcttagat atggattaaa
     8581 tgaatttact ataaaagaag gtagaacatt ttgggacgag ttgggagaaa gtttgacaga
     8641 acctatgata ttaattctca taggagcagc agtaataagt tcttttgtgg gagaattaca
     8701 tgacgcatta ggtattttag gggctatttt tattggaatt tctataggca taataactga
     8761 aggaaaatct aaaaaagcag ctcatgccct gtcaaaatta acagaaaata tagaagtaaa
     8821 ggtgcttaga aatgggaaga taattaaaat atcaaaaaat gatttagtac caggagatat
     8881 tgtatatata gaaactgggg atatgatacc agcagatgga agacttatac agtctataaa
     8941 tttgaaactt agagaagata tgctaacagg agaatctgat gatgtagcta agaatgctga
     9001 tgctgtatta gatatggaag tagtttattc taaaacagaa ataatcgaac aggatgcaat
     9061 acctgcaaag caagtcaata tggtatttgg agggactctt gtagcatatg gtaggggcat
     9121 aatggttgtc actcatactg gtgataagac agagatgggt aagatagcac agaatttatc
     9181 aaatgaagac cagcaaactc cacttcaaat taaattaggg aagttgggtg ctaaaatagc
     9241 gggaatatca ggcgtaatag ctactttatt atgtatgttt atgattatac agatgcagag
     9301 aaaaggtatg ttaattttag atacaagcag tgtattatct ttcttacaat cattagaacc
     9361 tgctaaaaat gcatatatgg tttgtattgc attaatagtt gcaactgttc cagaaggttt
     9421 gccaactatg ataaatatta ccttagctat aacaatgcaa aagatggcaa agataaatgc
     9481 attagtaaca aagaaagaag cctgtgaaac tataggttct gtttctgtaa tatgttccga
     9541 caaaacaggg actttaacac aaaacaaaat gatggtagag gtagcatatg ttgatggtaa
     9601 atatataagt ggaggagagt atcaaagtaa tagctatttt gaacaaaact gtatagtaaa
     9661 ttcaactgca gatatagaaa aagaagataa ttcatttaaa tatataggaa gcgcaacaga
     9721 gtgtgcatta cttttatatc ataatgataa gaactataat gaaatgagaa aacaaacgta
     9781 tttgatatca caaataccat ttagctcaga agaaaagaag atgtcaacat taattcgtca
     9841 agaggattca gatatattac tttcaaaagg tgctccagaa gtattattaa aaaaatgttc
     9901 ttatgtacaa caaggaaaaa atattgttcc aataactcca aaagtagaaa aaagtatttt
     9961 agatgagatt aagaagttac aaattaaatc tatgagaaca ttaggatttg cttataaaaa
    10021 aatgagtaat tctaaaacag aagttgcaat gacttcagaa ggtgaattga atttaatagg
    10081 aaattcaaga agttatatga aggaagataa tttagttttt agtggatttg taggaatagt
    10141 ggacccattg agagaagggg tcaaagattc cattgataaa gcatttaatg caggagtaga
    10201 tgtaaaaatg cttacaggag ataatataaa tacagcaaca gctattggaa atgaattagg
    10261 attattaaat gatgggaaaa aggcagttga agcaacatat atagatgtat tgacagataa
    10321 agaattgaga gaagaaatca aaggaatatc tattgttgca agaagtaaac cagatacaaa
    10381 aatgagaata gtttcagcac ttcaaaaaag tggcgaagtt gtagctgtaa caggtgatgg
    10441 tataaatgat gccccagcac ttagtcaagc agatgttgga attgcaatgg gtatttcagg
    10501 aacagaagta tctaaaaatg cagcagatat aatattgact gatgatagct ttagtactat
    10561 agtagaggga ataaaatggg gaagaggtat atatgagaac tttcaaagat ttatacagtt
    10621 tcagcttact gtaaatatag tagcatttat aattgctata atatctcaac ttactggtaa
    10681 ggatatgcca tttactacaa tacaattatt atgggtaaat ataataatgg atggtcctcc
    10741 agccctagca ttaggattag aacctgttag agattatgta ttgaaaagaa aaccaataaa
    10801 tagacattct ggaataatag ctaggtctat gtttgtaaat attattataa atgctattct
    10861 tataattaca attgtattta ctcaatcagc ttttaatata ttgggagcta catcagaaga
    10921 acaaggtact gttatatttt ctttatttgc attcagtgcc ctttttaatg cattaaattg
    10981 tagagagttt ggtttaaata gtacaatacc aaatttcttt aaaaataaac tggcattgca
    11041 aataatagta gttacaggga taattcaaat aatatttaca caagtgttcc agagtttctt
    11101 taattctgtt tctttggact ttgatatgtg gataaagata atactatttg catccactat
    11161 cctattgtca aatgaatttg taaagctaat tcttagaaca atgaaaaata gtagaactat
    11221 gaactttaat agtaaaaatt aattcctatg aaaccgcaaa gtactatata ttgtaacttt
    11281 gcggtttttg tttgtttaat gttgttttat attaccattt tcatatttaa ttttttatta
    11341 atctaaacac aatcttccat tgtaattgct tcatctgtgt caacacattc aaagtcaata
    11401 tctaattttt gtaatatttt gtatgcctct ttttgcaaag gagtatcaaa ttcaataggt
    11461 gattttgtgt aaatataact gataaaaaac atgatttatt tcattccttt ccatttaaaa
    11521 acttatagtt atagtgtatc ataaggttat aaaatataga taaatatagt ataaatttaa
    11581 aatatgtaca gacaaaacaa aaaaatcata ttctaagtaa tacttgggaa aaatgatatt
    11641 atcataataa ataatctttg taaaaactta tatagaatct attaaacaga cactaagcca
    11701 taaaaacttt actaaatata aaattcgtat ctaaatagat atatagaata atattttact
    11761 gaagtagagt gatttttaag aattttatat ataaattatg ttatcataaa taaagatata
    11821 gattataaaa tttgttaata aaataaaggg cagggaggta agtttcatgt tttccaaaat
    11881 cctagtcaaa acatttatca gagatagcga aaacgttcaa aatacagatg ttagaaataa
    11941 gtatggttat gtagctggtg tagtaggtat attatccaat ttacttttat ttgtgataaa
    12001 ggtattcata ggaatgctca catctagtat agctataatg gctgatgcat ttaataatct
    12061 ttctgatatg gcatcttcag caataactat gataggtttt aaattagcaa gtaagccagc
    12121 tgataaagaa cacccttttg gtcatggaag aatagagtat ttatcagcat taatagtagc
    12181 atttatggtg atgttggttg gacttcaatt tgtaaagtca tctatagaaa ggatagtaaa
    12241 tccaattcct gtaaaatttg aagtaatacc acttatactt ttaattgcat ctataatgat
    12301 taaaatctgg ttgagtcgtt ttaataaatt tatgggaaat aaaattgatt cttcagcctt
    12361 aaaagcagtt tcacttgatg ctttaggaga tgtatttact tcaagctgtg tagttatatc
    12421 atttattgta gctagattta ctaattttcc tatagatgga tatgttggta tcgtagtatc
    12481 attagtgata ctttatgctg gtttctctct tgtaaaagat actataaatc ctcttttagg
    12541 ggaagcacca gatgaagaaa tggtaaattc tattatagag ctattgttat cttataaata
    12601 tataataggt acacatgatt taattattca taactatgga gttggtagat gtattgcatc
    12661 tatacatgca gaaattccat ccaacataga tataatggaa atacatgaaa ttatagatac
    12721 tgcagagaga gaaatatctg aaaaattaga tatatatcta gtaattcaca tggacccaat
    12781 atgtcttgaa gataaagaag ttatgtcagc caaaaaagaa cttgaagaaa tacttaagaa
    12841 aaatagtctt gtaaaatcta tgcatgattt tagaatagtt ggaaaaggaa gcaagaagaa
    12901 tttaatattt gatatagtag ttaatccttc cgaattttcc aaaaacatgt cagaagatga
    12961 cttaaaagaa gatataacaa aattagttaa ggagataaat cctgaatata attgtgtaat
    13021 tgttgtagat aaggatttta tttaattatt aattaatctc atcataaggt aaaaaatgta
    13081 aaaaggatag acaatagtat agaggcataa tataatagaa gtatataatt gtatttttaa
    13141 tataaataaa gcaattttaa ttgtttttat gaaatattat agagatgttg aagtatattc
    13201 tattattggg caccttatgg atatactgag tcagtggtgc aaccggctat gaatataaat
    13261 ttatttattt tcatagcttt ttttgtataa ttttttagta aaaattactt tatatacgat
    13321 attaatggaa gattagatta taaattatag gtaacctaaa ggggtgagat ttacatgaag
    13381 aaaggaaata gaaaggcatt attaattagt ttaattatga ttttaagtat ggtggtgtct
    13441 acaatatatc caactgtatc ttatgcttca gaattaggag agaatagtca gattcaaagt
    13501 ggttcaacta attcatcaac tggagaggag aaggaaagcg acaataaaaa gccagaacag
    13561 actccagaga aagataaggc aacagataat aaaaaaccag aacagacccc agaggaagaa
    13621 aaaccaacag ataataaaaa accagaacag accccagaag aagataagtc aacagataat
    13681 aaaaagccag agcaaactcc agaaggagaa aagccaatag ataataaaaa gccagagcag
    13741 ataccagaag aagataagtc aacagataat aaaaagtcag aacaagcttt agaagatgaa
    13801 aaaccactag acaataaaaa tacagaaaaa actccagagg aagataatct attagaagat
    13861 gaaaatttat tgaaagtttt agaagaagaa cttaatgaag aaaatgaaga ctatggattt
    13921 gttgtaaaaa ttaataacaa tacaatagaa acggaatcaa tgaaaaaaat atcatttaat
    13981 ttaacttata ctccaacatc aaaaggaata caagcaggag attcaataac ttttaaggtg
    14041 ccagatgtat tcaataaagt taatttagat tatacaagtg aatgctttga taaaacagaa
    14101 agtaatggag agtatacact tacatttaga gaattaccta atggccaaag tgtaatgcaa
    14161 ggtaaaatag gtttggaagc atatgttaaa aaggttgatg aagatactaa tgcaaaaata
    14221 catattgaaa ctactggaaa aatagaaagt ggaagtggag atatagatgt agaaataaaa
    14281 cctggagata aaacggatgt tccagatgct aaaggaacac ttaaaaagct tgttgaagga
    14341 aaaaaatcaa ctactgtatt tatgccagtt aaaaataaag atataaatta cagtatacaa
    14401 gtaaatgaaa agcaggaaga gttaaaagat attattttgt atgatgagct tcctgaagga
    14461 ttgacattga taaatggttc tgtaagtgta gtaacaagtg atggaaaaga agttagtgat
    14521 tttaatattg aacagagtaa aaattctatt tcagttaatt ttggaaatat agataaatcg
    14581 tatactgtta agtataaggc tagaatcagt gacaaaaacg caaaacatgg taataaatat
    14641 aagaatgtag ctaggattga atctgatggt aagaagatac aagaagatga tgctactgta
    14701 agtatatttg atagaggaga tgattatctt cttactaaag gacattcagg agcaacaaat
    14761 attactcaag ttggtcaagt aataaactat cagatttcaa taaatgatga taaatcacca
    14821 ataagtaatg ttgttataac agataatata ccagaaggca tgagattaac tacttcagga
    14881 gaagctgggc atgattttag agttgtagaa attccaatga atgggagctg gactccttgg
    14941 agtaaagaaa aaattgccaa taatatttca tataaagtag aagaaaaaag aaatgaaagt
    15001 gggcaagtag ataaagttat aactggattt acaattaatc tcagtaaaga agaagtggaa
    15061 tcaaagtttt ttatagctta tacattaaag gtaattagta ttgaagatag ctatataaat
    15121 agagctgttc ttgatgcaaa taatagtgaa attgataaaa atgatgagat aaactttaag
    15181 aaaaattctg gattaataag cgcaaaaaaa gaagttgata aaaaagtttt aaatagtagt
    15241 gataaccaga ttgttaaata caaaatcaat atgtcaactt atggagttta tgatgcagga
    15301 caagtaaatc ttttagatga agtaaactca gctctagaaa tatctaatat aaaatattct
    15361 gataatcttg aacttaaaaa agaagctgga gatggaaaaa atacaataag attagtaaat
    15421 aaatacgaat ttaaacagat aaaagaaggg gaacctgtgc aaagttgggt tacatttgat
    15481 gcagacttca caaatgtaaa agttggggag actatacaaa atgtggctca gattaatggt
    15541 tcaagtcctc caggagtaga gactactaag caaggttatg cttttgaagc taaaaaagtg
    15601 gatgctttag ataaaaatgt actttcaggt gctaagttca atttagaaga tgcatttgga
    15661 aatatagtag ttaaggattt agttagtgat gaagatggta taattcaatc tagtataaaa
    15721 aaaccaggaa catattattt agtggagata atggctccaa gtggatatga aaaactgaag
    15781 gataaaatta aggttgaaat tggtaataaa gatattggta gaatagtgga tattggaaat
    15841 atagaaaaca taagacaaga aaatccacca gtaaatccac cagcaccacc aaatacagat
    15901 gaaccaatag taaatccacc agtaccacca agcacagata aaccaaggaa accatcaagt
    15961 tcatctgata cagaggatac tatagttata aatccaccaa taccaccatc agaagatata
    16021 ataaatccgc caataccaga ggtattgaat ccgccagtac caccatcaga agagatgata
    16081 gaaactcctg ttaaacagat tattccaata ccagaagtag taaagccatc agtgtcagaa
    16141 gaaaaaaata ataaagttaa agatgatact ttggtaaatc caccagtacc accaaaaaca
    16201 ggggatagta ctacaattat tggagaaata cttttggtta taggagctat tgtaggactt
    16261 atcgtattaa gaagaaataa aaatacaaat tagaccctaa cattataaaa aatataagtt
    16321 atattaaaaa atatcttcta aaacaatatt taataatgaa aatattgtta gaagatattt
    16381 ttttttgaag atttatgtaa aaaaataata actttttaga aaaaagttcc aaagggatat
    16441 agacaattaa aaagatataa gctataatat agatataaaa ttaaatgagg caaaactagg
    16501 gaaacctagt cacgcaaagc tataggggct aaagtttagt aaaccatgcc agccagttgc
    16561 cgaaaagagt aaaaatttct ttttgtatat acaaagatac tcttgggtat cttttttatt
    16621 ttgaaattta ttactcccgg cctaactcaa aggagggaat aatatgagac caagtaaaaa
    16681 attattaata gctataatat caatattctt aataagttca gttccagtaa gtgcacatgc
    16741 agatagtact actatacaac aaaataaaga cacacttagt caaatagtag tttttccaac
    16801 tggaaattac gataaaaacg aagctaatgc tatggtaaat agattagcta atatagatgg
    16861 aaagtattta aatgcattaa agcaaaataa cttaaaaata aaattattaa gtggaaagtt
    16921 aacagatgaa aaagaatatg cttatttaaa aggtgtagtt cctaagggat gggaaggtac
    16981 tggaaaaact tgggatgatg ttccaggttt aggtggaagt acagtagctc ttagaatagg
    17041 atttagtaac aaaggtaaag ggcatgatgc aataaattta gaattacatg aaacagcaca
    17101 tgcaatagac catatagtat taaatgatat atctaaatca gcacaattca aacaaatatt
    17161 tgctaaagaa ggtcgttctt taggtaatgt aaactactta ggtgtatatg cagaagaatt
    17221 ttttgcagaa tcatttgcat attactattt aaatcaagat acaaatagta agttaaaatc
    17281 agcttgtcca caaacttata gctttttgca aaatttagct aaatagagtt ttaataaatt
    17341 taaaataaat ataaataaag agatgctatt ataatagcat ctcttttata ttgcgtttaa
    17401 atctaataaa ttttgtctta tgtataacta tgatagatta tcaattacta ctccttatac
    17461 aaggcttcaa cgtgtttatt atgcatttga gtaattatcg taggtataat agaaattatt
    17521 gaaccaatta ctatcaataa aattcctata accatgttta tagggataat ttctttaagt
    17581 attaataatg ctagtattgg agatattact ggtttaaaga agaatactaa agatgtaaga
    17641 ctagtagatg tagcttccat tgccataaag taaagcgcat aaccaacacc tgtaacaaat
    17701 acgaatacat aaatcataat gggcatattg tgtaaggtat atccactaaa aatagggata
    17761 tttgcaaata catgaagctt atttttattt aatatatctg cgataaaatt aattttagtg
    17821 agtaaaacca atatcaacat ttccaaacta ccaaatataa agctaaagca ggttacaact
    17881 attccaccaa attttaaggt ttttctttta ccaaatacag tatacattga aaaaataata
    17941 gcagaagata atgttaagaa tattccacta agagttagtt ttgtatgcaa tggatttatt
    18001 ataactataa ttccaagaac ttctaatata agtgaaatta cattgtgcct atatattttt
    18061 tccttcaaaa ttaagtaagc taaaagcatt acaaagactg gattacaact gaacaaaaca
    18121 gcaactacag atgcctttgt gtttagaaca gcaagttgat acaatgacat acttactact
    18181 acacacataa aacctaaaaa tgcaaagtat tttaagtctg attttgtaat agaaagacta
    18241 agagctctta atctttttac agcaaaaggt agtaagacta aaccgccaac aaaaaatctt
    18301 gataaagtta tttgtaaggg attaaagtca ttagtgacag tttttagact aatttccatt
    18361 gaactaaaaa ataaggtagt cagtattata taaacataac cttttttcaa gtcaaacacc
    18421 tccttgtaat ttatattagt actatagata agaatattca agcatattgg aataatatgg
    18481 tatgtataaa atatagtaca taaaattata aactacctta atatagaatg taagtattct
    18541 atactaaggt agttaattat ttgaattact ttgatttagt tattttaaat tatataattt
    18601 ggctaatttt tcaaatgctg gtaaagaact tttaacttta tcataagata cacaatatga
    18661 tactctaaaa tgacctggac aaccaaaaga agaacctggt actaataata aattaaattg
    18721 ttttgcatca ttgcagaatt ttttatcatc ttcaattgga gcttttggga acaaatagaa
    18781 tgtaccatta ggtttaacac aagaatatcc catatcgatt aaactattat atagaaggtc
    18841 aaaattcttt ttataaatat ttacatcaac ttctgcatct aaacttcttg caataaccct
    18901 ttgaaaaaga gaaggagcat ttacaaatcc aagtattcta tttgcaacat ttaatgaaga
    18961 cataacatca tcaaaatcat ccatttgccc attagcaaca atatatccta tacgttcacc
    19021 aggtagagat aatgatttac tataagagta tcctataaat gaatttctat agtattttag
    19081 tacacaagga actttggcac catcataaat tatttctcta tatggctcat cagaaattag
    19141 gtatatgctt gtattaaatt ctttttgttt tgaatccaat aattctccaa gattttttaa
    19201 taattcttca gaatatatga caccagtagg attatttgga gtgtttatta ttaaagctct
    19261 tgtttttgga gtaatagcat tttttagagc atctaaatct ggctcaaaag tctctatatt
    19321 tgtaggaact tctattaatt ttccatcata gttttctgta taatttttat actcaccaaa
    19381 atacggtgca aaagcaataa cttcatcacc aggatttaaa agagttttta aaataatatt
    19441 tagaccacca gcagcaccac aagtcataat taaattttct cttgtaagat ttaatccatc
    19501 ttttttattt atgtgttcag caatagcatc tcttacatcc tcatatcctg agttgttcat
    19561 atatccatga actaaatttg gagattcttc atttaaaata tcatttataa ctgctttaat
    19621 agtctctgga ggttcaacac ttggatttcc tatactaaaa tcaaaaacat tttcctcacc
    19681 ataaatatca gaaagtttct ttccttcttc aaacatagct cttataacag aactatttgc
    19741 aactaaagtt tgcattttat tagatatcat atgtaacact cctttttata gatttatatt
    19801 gcttatgtat atattaccat atttttactg aatttagaca attttcgaaa aattacttgt
    19861 ttgaaaatat aaacttttat ataaaatata tttatgtaca gtacatatta aaaattccaa
    19921 tttctaattt tat
//
LOCUS       DAMJVI010000072        19752 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.074, whole genome shotgun sequence.
ACCESSION   DAMJVI010000072 DAMJVI010000000
VERSION     DAMJVI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19752
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.074"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            23..1090
                     /locus_tag="PC545_002540"
     CDS             23..1090
                     /locus_tag="PC545_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HDF2457304.1"
                     /translation="MKNKLVYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFEKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSIANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVMGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPNIIPTSITSSNSRNNYQPKIL
                     DGSEKDRVLNKLEKISKDLNK"
     gene            1260..3161
                     /gene="cooS"
                     /locus_tag="PC545_002541"
     CDS             1260..3161
                     /gene="cooS"
                     /locus_tag="PC545_002541"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HDF2457305.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVVKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFKLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAECIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3154..3627
                     /locus_tag="PC545_002542"
     CDS             3154..3627
                     /locus_tag="PC545_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2457306.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSRHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3602..4828
                     /locus_tag="PC545_002543"
     CDS             3602..4828
                     /locus_tag="PC545_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2457307.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENVYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVKWIAGKTVKGIDINKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLRDGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIVLDNSKNVT
                     GVAFSNGEVLDADMVIVATGVKPNADFLDGTGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMVGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGINDYKKFVFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDICY
                     ADFFNIKENGEFCYSV"
     gene            complement(4967..6154)
                     /locus_tag="PC545_002544"
     CDS             complement(4967..6154)
                     /locus_tag="PC545_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2457308.1"
                     /translation="MNITKNFLKDYLEKFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTREYMYN
                     CSK"
     gene            6427..7251
                     /locus_tag="PC545_002545"
     CDS             6427..7251
                     /locus_tag="PC545_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDF2457309.1"
                     /translation="MEVYALVGSSGTGKSFKALEFAYENDIEYIIDDGILIYKNKVLA
                     GISAKQANTTIEAVKRAIFYNLEHRQEVREAIKKENIRKILIIGTSKKMVNQIVERLS
                     IGRVCKFINIQDISTKYEIEIAKQARKEGNHVIPVPAVEIKSMASGLSINSLKRLFRK
                     GNNRNMVVLEKTIIRPTFSYIGKFYISADVIKQIIEYEVYNFGSIDRINKINIENCNN
                     FMNIFVSININDLETIKKIEDIQQTIKKSIEKMTLVNVQKIDINIHKIKKINFLQC"
     gene            7380..8645
                     /gene="gluD"
                     /locus_tag="PC545_002546"
     CDS             7380..8645
                     /gene="gluD"
                     /locus_tag="PC545_002546"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HDF2457310.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            8858..9448
                     /locus_tag="PC545_002547"
     CDS             8858..9448
                     /locus_tag="PC545_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2457311.1"
                     /translation="MHFKSKDLNLKYLVLFLKIVYWFNPFICIMEKYMDLDCELYCDE
                     RVLKNCTVQQKQEYALTIVNSMKRSTNFSNKFVAGLNKQSDIRKRVINMFSEKHKNGI
                     LRALIISLFSSITFFKFDSTSIIEENDFLERSRYTFEGRQEDMFVTIDFIYADVPIKL
                     RKEHEENCRVNGIVPKDSDSIEIIPRFYKDCMDLKK"
     gene            9778..10911
                     /locus_tag="PC545_002548"
     CDS             9778..10911
                     /locus_tag="PC545_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HDF2457312.1"
                     /translation="MSNFILNGIFRTVIVSSLSILLILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKHININNINLEGFNNVSTISNNIILYTWIITVVVY
                     LIYTIFKYIKLKNLINDLSYDVDDREIVNLYKNILEEFNITKDIKLKYSYEVETPAFF
                     NSCVLLPPHEYKLKELDWIFRHELMHFKSRDLYLKYVILFLKTVYWFNPFIYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTILNAMRKGSNTSNKFIAGLHKQSDIKKRVMN
                     MFNEKYKNGILMALTLCLLSSITFLKVNSTSIVNLNVLPKGFEDLRTVSSIRFSVIGF
                     TYADAPKNMREEYKINCESLGLIPKDSDKIEVGIEYYDSLLND"
     gene            11827..12849
                     /locus_tag="PC545_002549"
     CDS             11827..12849
                     /locus_tag="PC545_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDF2457313.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEDNGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQVNPSIDQNVDSTNNNQQNNANTNQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVSVTSINSSYYTARFV
                     TAKRIL"
     gene            13146..14066
                     /gene="pyrB"
                     /locus_tag="PC545_002550"
     CDS             13146..14066
                     /gene="pyrB"
                     /locus_tag="PC545_002550"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDF2457314.1"
                     /translation="MLKSRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMRIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLESHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDS
                     DDRAVYFKQAKYGMYVRMALIIKLLGINED"
     gene            14105..14797
                     /locus_tag="PC545_002551"
     CDS             14105..14797
                     /locus_tag="PC545_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDF2457315.1"
                     /translation="MYKILENIYIGEDMYRMKVKGNFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEDEYKQVCNKVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNGGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            14790..15692
                     /locus_tag="PC545_002552"
     CDS             14790..15692
                     /locus_tag="PC545_002552"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2457316.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDYELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVGMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            15777..16361
                     /locus_tag="PC545_002553"
     CDS             15777..16361
                     /locus_tag="PC545_002553"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDF2457317.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            16576..17502
                     /locus_tag="PC545_002554"
     CDS             16576..17502
                     /locus_tag="PC545_002554"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDF2457318.1"
                     /translation="MKNQLNENHKKVVFFGVGAVGATFAEQFFNSKYDFKILCDNERK
                     KRYLEEGFIINGKRYDFDYVTKDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYTLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKDISLTEDDVEHSLHRILEHSKEGRTSMLQDVEAHRLTEVDMF
                     SKNICKLGKKYNIPTPINQTFFYMIKVIESRF"
     gene            complement(17554..18444)
                     /locus_tag="PC545_002555"
     CDS             complement(17554..18444)
                     /locus_tag="PC545_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457319.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSKKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQNQKWIVREDGSGTRDYLDMFLSVKEIIPKSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            18632..19663
                     /locus_tag="PC545_002556"
     CDS             18632..19663
                     /locus_tag="PC545_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDF2457320.1"
                     /translation="MLKKKSSLTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKIVRVIFLVVVVLVFGHMKNQSNREILEEEKDEIKKKKVTIPWYVIGFFITCALF
                     SMNIIPQEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFVGIVQVVS
                     AVVLIKIFI"
BASE COUNT         7756 a         2088 c         3498 g         6410 t
ORIGIN      
        1 attaatacaa aggagattta ctatgaaaaa taaattagtt tacatatgta ttatgagtct
       61 attgctattt gtagtaggat gttctaataa tccaaataag gaaagtacca ttaaaaaaga
      121 ctctaagaat aaccaaagcc aagttaaaga agaaaaaaga gaacctgtga aaaaagatat
      181 aactataagt ttcgctggtg atgtaactat ggggaactat aaaggttcat catattatgg
      241 cagttttgac catgaattta aaagacaagg agaagattac aattattttc ttaaaaatgt
      301 aaaacctgta tttgaaaaag atgacttaac agtggttaat ttagaaggtc cacttactac
      361 agcgtcaaat gcaaaagaaa aaaaatttgc atttaaagga aagcctggat atgtaaatat
      421 tttaaaatct ggaagtgtag aggcagtaag tattgcaaat aatcattcag aagattattt
      481 tgaagaagga atgaaagata cacagtttat attggacgag aataaaataa attattttgg
      541 attagggaaa gatgctgttg tagatgtcaa aggaattaaa gtgggtctat tagggtataa
      601 tggattatca actgaatata atgaaaaaaa tttaaaacaa atggaagatg atattaagtc
      661 cttaaaaaag aaatcggatg ttgttgtagt gtactttcat tgggggatag aattagatta
      721 ttatccagat aaaaaacaaa aagattttgc tcattatgct atagacaagg gagcagattt
      781 agttatgggg tctcacccac atgtgattca aggaatagaa aagtataaaa ataagtatat
      841 agcttatagt ctagggaatt tttgctttgg aggaaataag aatccttctg atacagatag
      901 ttatatatat caacaaacct ttactttttc agataataaa ttaacttcaa ttaaagagcc
      961 aaatataata ccaacttcaa taacatctag taactccaga aataattatc aaccaaaaat
     1021 attggatgga agtgagaagg atagagtttt aaataaactt gaaaaaataa gcaaagattt
     1081 aaataaataa aatgtaaaaa aaaataaata tatatattta ttttaataat ttatattttg
     1141 tttagggtat ggtaaatcgt ggtattaaat aagtagattt taatagttaa attatagtca
     1201 attataataa agctatttaa tctacttttt taatataaaa atataagggg gaatttgtaa
     1261 tggcttgtaa cacttgtaaa atgtgcgaaa gtgcagacaa gaaattagaa agttttgtag
     1321 cttcaaagga tgttgaaaca gcatttcata gaacagaaga tcagaaagtt aagtgtggtt
     1381 ttggacttca gggggtttgt tgtagactct gttcaaatgg accatgtaga gtaactccta
     1441 aatcaccaag aggtatatgt ggagcagatg cagacactat agtagcaaga aactttttaa
     1501 gagcagtagc atcaggagca gcttgttatt tacatgtggt agaaaataca gctaagaatt
     1561 taaaaaatgt tggaataact aaaggtgttg ttaaaggaga aaaaacatta aatcaattag
     1621 cagagatgtt tggaatagaa agtgatgaaa agtatgataa atgtataaag gttgcagaca
     1681 aagttatcaa cgatttgtat aaatcaagag atgataaaat ggaattagta gaaaaaatag
     1741 cttatgcacc tagagtgaaa aagtggaaag agttaggtat aatgcctggt ggtgcaaagt
     1801 cagaagtttt tgatgcaata gtaaaatcat caacaaattt aaacagtgac ccagtagaca
     1861 tgctagttaa ttgtttgaac ttaggtatat caacaggatt atatggtctt acactaacta
     1921 atcttttaaa tgatgttatg ttgggagaac cagttataag aatggctcct gtaggattta
     1981 atgtaataga cccagactac ataaatataa tgataacagg tcatcaacat tcaacatttg
     2041 caaatttcca agatagatta aaagatgaag atgttataaa attagctcaa tctgttggcg
     2101 caaaaggatt taagttagta ggatgtacat gtgttggtca agatttacaa ttaagaggag
     2161 aacattatca agaagtattt gctggacatg caggtaacaa ttttacaagt gaggctgttt
     2221 tatcaactgg agcaatagat atagttcttt cagaatttaa ctgtactata ccagggcttg
     2281 aaccaatagc agataaatat aaagtaaaaa tggtttgttt agatgatgtt gctaaaaaag
     2341 cgaatgcaga atgcataggt ttagacagaa gtaaattaga tgaacttagt aacactttaa
     2401 tagaaaaagc attggagtct tataaggaga gaagaggaag tattgaaata gatataccaa
     2461 aagaccatgg atttgaacaa tctttgactg gtgtaagtga aaagaacttg aaagcgtttt
     2521 taggagattc atggaaacct cttattaatt taattgcaga aggaaaaatt aaaggtgttg
     2581 cagctgttgt aggatgttca aatatgacag caggaggaca tgatgtaaat actgttgaac
     2641 ttactaaaga attgattaaa aaagatataa tagtactatc tgcaggatgt tctactggag
     2701 gattagaaaa tgttggtctt atgtcacctg gagcagaaga attggcagga gaaaacttaa
     2761 aagaagtatg taagacttta ggaataccac cagtattaaa ctttggacca tgtctagcta
     2821 taggaagact agaaatagta gcaacagaat tagcagctga acttggaatt gacctaccac
     2881 aattaccatt agtgctttca gctccacaat ggttggaaga acaagcatta gctgatggag
     2941 cttttggtct tgcccttggc ttacctcttc atttagcttt acctccattt ataacaggag
     3001 gaaaattggt aacagaagta ttaacagaaa aattaaaaga cttaacagga ggacatgtta
     3061 tagtgaatcc agaccctaaa tcttcagcta atcaattaga agaaataata atagatagaa
     3121 gaaaaaattt agggttaaag gatgtggaat gcaatgcata gaatttttat aaataaagat
     3181 ttatgtacag gatgtaagag ctgtgtacta gcatgtatgc taaaacacaa taaagattat
     3241 gacatgtaca ctcttgattt agaaaatata gataatgata gtagagggca tatagaatta
     3301 gatagtagac ataataatcc agttccaatt ctttgtagac actgtgatga acctgaatgt
     3361 gtattagcat gtatgagtgg tgcaatgcat aaagatagtg aaagtggaat cgtttcatat
     3421 gatgaagaga aatgtggttc atgttatatg tgtgtaatgt cttgtccata tggattatta
     3481 aagccagatg atagaagcaa gcagaacata cttaaatgtg atttatgtaa agatgaggag
     3541 tatcctagat gtgtagctaa ttgcccaagt ggagcaattg aattacagaa ggaggaaaat
     3601 gatgaattat gtagtgttag gggctagtgc agctggtata aatgcagtaa aaacattaag
     3661 agaattagat aaagattcaa atatagtagt aatttctaaa gatgaaaacg tatattcaag
     3721 atgtatgttg catcacgtta tatctgaaca cagaacttta aaacaaataa attttgttga
     3781 tgaagatttt atggagcaaa ataatgtaaa atggattgca ggtaagactg ttaaaggtat
     3841 agacataaat aaaaaggtag ttcagacaga agacattact gtaaattatg ataaactttt
     3901 aatagctaca ggtgcatctt ctgctattcc tcctataaaa aatttaagag atggaaattt
     3961 tgtatattct gtgagaaaca tagatgatat ttataaaata aaagaaaaag cacaaaattc
     4021 taaaaatgta gttataatag gtgctggttt agttggtata gatgcattag tgggattatt
     4081 taaatatgag cagttaaata tttctgtagc ttttatggaa aagtacattt tagatagaca
     4141 attggatgaa tatacagcat cagtgtatga aaataagttc aaagaaaaag gtgtaaaatt
     4201 ttacccaagt gcatcaatac aagaaattgt attggataac agtaaaaatg ttacaggtgt
     4261 tgcattttca aatggtgaag ttttagatgc agacatggta attgtagcta caggggttaa
     4321 accaaatgct gactttttag atggaacagg aatagaatat gatagaggta taataataga
     4381 tgatatgtgc caaacaacac aaaaagacat atatgcagca ggagatgttg taggtaaaaa
     4441 tgctatttgg cctctggcag taaagcaagg aattgtagct gcctataata tggttggaaa
     4501 agataagaag atagaagatg aatttgcttt taaaaatagt atgaatttca tggatatacc
     4561 aactatttct ataggaatga atacacctgt agatgatagc tataaagtat taaccagaca
     4621 tggaattaat gattataaaa agtttgtttt taaagataat gtaatctatg gagcagttat
     4681 acaaggagat atttcatatg taggtgtatt aacttattta ataaaaaata aagttgaaat
     4741 atatgattta gaaaatagga tatttgatat atgttatgca gactttttta atataaaaga
     4801 aaatggagaa ttttgttata gtgtatagta tatacgtgga gtaaggttta taaatacagt
     4861 aaatcaataa atagagttat tctaaaataa gaaaaatgac aaatcttatt tagaaatagc
     4921 tctatttttt gtaattaaat ttaaattata ttcaatttgt aaaatattat tttgaacaat
     4981 tatacatata ctctctagtt agaggtaaat cattttttat acctttagtg aaaagtattt
     5041 gatgtatatc tataatacca gtgttgaaag cagcagcaca agaacaaagg tataaatccc
     5101 acattcttat aaacttatca tcaaacattt ctttaatctt tggaagattc tcagagaaat
     5161 ttttttgcca gtatagtaag gtttttacat aatgtaatct taaactttct acgtctatag
     5221 tatgatattt gtaatcacta caaatatgta tcatttctct taggctagga ataactcctc
     5281 ctgggaaaat atattttttt atccaagggt ctccataaga ttcctgcaat ccacttatat
     5341 agtgaagtaa aaacaatcca ccatctttta aaacagcatt tacgttttta atgaaaagtt
     5401 catagttttc tctaccaaca tgttcaagca tcccaacgct tacaactcta tcaaacataa
     5461 ggtctgattt ttttaattct ctatagtcca taagtcttac ctcaagatag tcttgaaggt
     5521 tctcttcttt aattcttgcg ttaaattcct taaattgctc ttcgctaagt gtaattccaa
     5581 cgccgtgaat tttatatttt tttgcagctt ctattagtaa aaatccccaa ccacaaccaa
     5641 tatcgagaag gctcatacct tcctctaaat ttagtttagt taatatgtgg tgaactttat
     5701 tgacttgagc atcataaaga gtatcattat catttctaaa gtatgcacaa gaataactaa
     5761 gtgtcttatc taaccataag ctataaaaat cgttgccaat gtcatagtgg gcatgtattt
     5821 ctttcttttg atgtctcatt gaatttgatg agtgcaatag atgttttaca gctgaattat
     5881 caatattgaa tttgtctatt tgttttaaaa gtagatttaa agttaagaat aaatctccct
     5941 caacttcaat gtctctattt atgtaggctt ctccaagtgc taaagatgta ctttttaata
     6001 gttcagactt gctaatatca ttatttacta taactttgaa ctgaggggag ccctctccaa
     6061 ttagaaattc ttcctcatct ttaagcctaa ctagaaatgg tgtttcacca aatttctcta
     6121 aatagtcttt taaaaaattt tttgtaatat tcatattacc agctccttct aatgtaaatc
     6181 cagtaagttt ccctgcttaa aatatattat agtccttttt ttaaaaaagt aaaatttttt
     6241 caataaattt tttatttaaa ggaaatttat tttttgtaaa gaaatttcat gttgtagtat
     6301 attttttgct tataaatttt ggtactggat ataatattaa actgttaatg atataataaa
     6361 tttaatctat aaagtaaata aaattttgta ataaatagtt aaagctattt tataaggaga
     6421 tatatgatgg aagtatatgc tttggttggg tcaagtggca cagggaagag ctttaaggct
     6481 ttagagttcg catatgaaaa tgatatagaa tatataattg atgatgggat tttaatatac
     6541 aaaaacaagg ttttagctgg gatatcggca aaacaagcaa atactactat agaagcagtt
     6601 aaaagggcta ttttttataa tctggagcat agacaagaag ttagagaagc aattaaaaaa
     6661 gaaaatataa gaaaaattct tattattggg acatcaaaaa aaatggttaa tcaaattgta
     6721 gagagattgt caattggaag ggtttgcaag tttataaata tacaagatat aagtactaaa
     6781 tatgaaatag aaatagcaaa acaagctaga aaagaaggaa atcatgtaat tcctgttcca
     6841 gcagttgaga taaaaagtat ggcaagtggt cttagtataa attctttgaa gagacttttt
     6901 agaaaaggaa ataataggaa tatggtagtt ttagaaaaaa caattataag accaactttt
     6961 agttatatag gaaagtttta tattagtgct gatgtaatta aacagattat tgaatatgaa
     7021 gtatacaact ttggaagcat tgatagaata aataaaatca atatagagaa ttgcaataat
     7081 tttatgaata tttttgtaag tataaatatc aatgatttag aaactataaa aaaaattgaa
     7141 gacatacaac agacaataaa aaagtctatt gagaaaatga ctttagttaa tgtacaaaaa
     7201 atagatatta acattcataa aattaaaaaa ataaacttcc tccaatgtta aaaaataaac
     7261 tgagaaaatg atatactaat ttgcaataaa gtgtgctata attaaactgt aaatttagga
     7321 aatggttttt tctaaatgga gtatacataa ttataacgta tttataaggg ggctttcaaa
     7381 tgtcaggaaa agatgtaaat gtcttcgaga tggcgcaatc tcaagtaaaa aatgcatgtg
     7441 ataaattagg tatggaacca gcagtttatg aattattaaa agaacctatg agagttatag
     7501 aggtttcaat tccagtaaaa atggatgatg gttctataaa aacttttaaa ggatttagat
     7561 cacaacataa tgatgcagta gggccaacaa aaggtggaat aagattccat caaaatgttt
     7621 caagagacga agtaaaagct ttatctatat ggatgacttt caagtgttct gtaacaggta
     7681 taccatatgg tggaggtaaa ggtggaataa tagtagatcc atcaacttta tctcaaggtg
     7741 aattagaaag attaagtaga ggatacatag atggaatata taaattaata ggtgagaaag
     7801 ttgacgttcc tgcaccagac gtaaatacta atggtcaaat aatgtcttgg atggttgatg
     7861 agtacaataa attaactgga caaagttcta taggtgttat aactggtaag ccagttgaat
     7921 ttggtggttc tttaggaaga acagctgcta ctggatttgg tgttgctgtt actgcaagag
     7981 aagctgctgc taaattagga atagatatga aaaaagctaa aatagctgtt caaggtatag
     8041 gaaacgttgg ttcttataca gttcttaact gtgaaaaact tggtggtact gttgtagcta
     8101 tggctgaatg gtgtaaatca gaaggttctt atgctatata caatgaaaat ggtttagatg
     8161 gtcaagctat gttagattat atgaaagaac atggtaactt attaaacttc ccaggagcta
     8221 agagaatatc tttagaagag ttctgggctt cagatgttga tatagtaata ccagctgcat
     8281 tagaaaactc tataactaaa gaagttgctg aatctataaa agctaaatta gtttgtgagg
     8341 ctgctaatgg accaactact ccagaggctg atgaagtatt tgctgaaaga ggaatagttc
     8401 ttactccaga tatattaact aatgctggtg gagttacagt ttcttacttc gagtgggtac
     8461 aaaacttata tggatactac tggtcagaag aagaagtaga gcaaaaagaa gaaatagcta
     8521 tggttaaagc atttgaatct atttggaaaa tcaaagaaga gtacaatgtt acaatgagag
     8581 aagctgctta catgcattca attaagaaag ttgctgaagc tatgaaatta agaggatggt
     8641 actaagattt actcttaata aataatagga tataattatt taagatataa aggggctgta
     8701 catagtacag cccttaaatt atgtggctta caatgaagta agctattttt gttttaactt
     8761 ataaataact tgataaatta tgtaatttag aatgtatttt ttaccaccac atgattacaa
     8821 attagaggag ttagattgaa tttttagaca tgaacttatg cattttaaaa gcaaagacct
     8881 taatttaaag tatctagtat tatttttaaa aatagtatat tggtttaatc catttatatg
     8941 tataatggaa aaatatatgg atttggattg tgaactttat tgtgatgaga gagttttaaa
     9001 aaattgtact gtacaacaaa agcaagaata tgctctaact attgtaaatt caatgaaaag
     9061 aagtacaaat ttttcaaata aatttgtagc tggattaaat aaacaatctg atattagaaa
     9121 aagagtaata aacatgttca gtgaaaaaca taaaaatggt atcttaaggg ctttgattat
     9181 aagcttgttt tcttctatta cattttttaa attcgattca actagtatta ttgaagagaa
     9241 tgatttctta gagaggtcaa gatatacgtt tgaaggaagg caagaagaca tgtttgtaac
     9301 aatagatttt atatatgcag atgtacctat aaaacttaga aaagagcatg aagaaaactg
     9361 tagagtcaat ggaatagtac caaaagattc agattcaata gaaattattc ctagatttta
     9421 taaagattgt atggatttaa aaaaataagt tagacttaat attgatgttg atattatgaa
     9481 ttttttatat ttaagcaatt gaaaatttta ttatagattc aattttatat gtgtttaagt
     9541 atgaaattta tagatagttg aaataattaa atatgattga tgcataggaa tcattactaa
     9601 gctataatta attatagggg agtttaaata aattatgagt tacacaatca taaaatgata
     9661 aaaatagtta tctgttacaa aaaatgtatc tttttaatta ataaagataa tatgaaaact
     9721 taaaaggcaa ataaacttaa atataaaata ctcttaaata agataggaga aaaatgtatg
     9781 tctaacttta ttctaaatgg gatttttaga acagttatag taagtagtct aagcattctt
     9841 ctgatattga ttttcaagaa aaatgtattt aaaagattta gcaaaaaatt taattattat
     9901 atatggatga tagttgttat taaattattt ttaccattta catattatac ttttacaata
     9961 aatatcttga gaagtaaaaa acacattaat attaataaca taaatttaga gggatttaat
    10021 aatgtttcaa ctataagtaa taatattata ttgtacactt ggattataac tgtagtagtg
    10081 tatttaattt acactatatt taaatatata aaacttaaaa atttaataaa tgacttatct
    10141 tatgatgttg atgatagaga gattgtaaat ctttataaaa atatattaga agaatttaat
    10201 ataacaaaag atataaaact caaatactct tatgaagttg aaacaccagc attttttaat
    10261 tcatgtgtac ttttgccacc acatgagtat aaattgaaag agttagactg gatttttaga
    10321 catgagctta tgcattttaa gagtagggac ctttatttaa aatatgtaat cctattttta
    10381 aaaacagtat attggtttaa tccatttata tatataatgg ataaacatat agatttagat
    10441 tgtgaacttt attgtgatga aagagtttta aaaaatcgaa attcagagga aaaaaaggat
    10501 tatgctttaa caattctaaa tgcaatgaga aaaggttcaa atacttcaaa taaatttata
    10561 gctggacttc ataagcaatc tgatattaaa aagagagtta tgaatatgtt taatgaaaag
    10621 tacaagaatg gtatattaat ggcattaact ttatgtttat tatcttctat tacttttttg
    10681 aaggttaatt caactagtat tgttaatctt aatgtattac ctaaaggatt tgaagactta
    10741 cgaactgtta gcagtatacg tttttcagtt ataggattta catatgcaga tgctcctaaa
    10801 aatatgaggg aagaatataa aataaattgt gaatcattag gtttgatacc taaagactct
    10861 gataaaatag aagttggtat agaatattat gacagtctat tgaatgatta aataaatatt
    10921 attttaacag atattaatta taaagttttg aatagtaatt tgttgagaaa taaatatatc
    10981 cttataaaaa tagatactta tataccaaga ataaaaaata gtcaagatag tacacaagga
    11041 tatacttata aagaacttaa ataagtgaaa taaattatta attataaggt ttataatttt
    11101 aaaaaattag ataaattcag gtgtaaaata ctataaaaca caattcggaa aagtgtatgt
    11161 tgaacttcat ttaaaatgga atttcaaagt tgctatagta agtagtttaa gcattcttct
    11221 tatattaatc tttgagaata atgtatttaa aaaagttagt aaaaaattta actattgtat
    11281 attaatgata gttgctattg atatattatc taaaaaaatt aagtatgaaa agattactgc
    11341 taggacaatc ttttcatact taatttttaa tatttatatt atccaaaatt ttcatctttt
    11401 taacttagaa tttgagaatc actattagga aataggcttt gatttatgta gggttaaaaa
    11461 tttcacagaa tatctaactt tatactaaat ccatcaaaaa atatacgcca tacaaaagcg
    11521 cataaaaaaa cacataaaac aatatttatg cgcactttaa cctcgaatgg tacaaaattc
    11581 tttactttta cagacccata ttatataatt aagtaaataa tgtatattta ggtgacatat
    11641 tggataagct aatttattta ttttattaat ataataatac tataaaagtt atataaagtg
    11701 gtttacaaaa aagttacaga tagagctttt attaatgtat tgtgtattag tattttatcc
    11761 ttaggtacac ttcacctaca aaatagaatc ataagataat tatgggaaaa ttttaggagg
    11821 tttatcgtga agaaaaagat attaatacca gtatttgcat cagttatggc attatcagta
    11881 agttcaattg taaatgcaga tgaagtaaat gattcatctc aaaataagga tgataaaaca
    11941 aatactgaat taaatttagg tgaatataaa gaagtaaaat ataaagttgc aaaaataaaa
    12001 gatggagtag ctataaagat aagagaagaa ggtcaagttc aaaacatagc ttattctgga
    12061 gatgaattta cggttttagg aactcaagga gaatgggtta aggttaaagt tgaagatggc
    12121 gaaggttggt tagctactag atatgtagat atatctgaag gtgtaggata tactaacgct
    12181 gataaagtaa acttaagaaa agataagagt gaaagttcag aagtaattga agagctggaa
    12241 aaaggaagtt ctttattggt tttggaagat aatggagatt ggttaaaagt taaagatggg
    12301 gaaacagaag gatatgtaaa atcatcttat atatctgata aagctcctgt aatagaagag
    12361 ccacaagtaa acccaagtat tgaccaaaat gtagattcta caaataataa tcaacaaaac
    12421 aatgcaaata ctaatcaaca aaataataat tcaaatgtgc caacagctaa ctctaatgca
    12481 gttcaagctg tacttaactt ggcatattct aagcaaggtt gtccatatgt ttggggagca
    12541 gaaggaccaa acacttttga ctgttctgga tttacacaat atgtgtatag aaatgcagta
    12601 ggaaagaata taccaagaac atctaaagca caatctaaat atggacaaac tgtaagtaaa
    12661 gcaaatttac aaccaggaga tttagtattc ttcacaacta atggttctgg ttctgttagt
    12721 catgttggaa tatatgtagg tgggggtaat atgatacact caccatcaac aggaaaaaca
    12781 gttagtgtaa ctagtataaa ttcatcatac tatacagcaa gatttgttac agcaaaaaga
    12841 atattataat cggtttgtaa tatgacaaat tgataaatac ataaatatat ataaacgctc
    12901 tatattatat ttaatttaat catggaaaca aaataatgat tatattttaa atgtactata
    12961 gagcgttttt gtatcacttt aaatctaatc aagattcaag ttaatatgac tgttaaatag
    13021 gagccttttc atatgatgat tatttgtgca atcccttgac aggatagggt aaaggtggta
    13081 agctactaac agtacaaaaa tacttttaat tttagttcag agaggctaaa aaaggaggac
    13141 gaaagatgtt aaaatcaaga aatttaatcc aaccagaaga cttttcaata gaagaaatag
    13201 atgaaatatt agaattagct caaaagataa ttgacaatcc gtcaaaatat tctcgtatat
    13261 gtgaaggaaa actattagct acattatttt atgaaccatc aacaagaaca agattaagct
    13321 ttgaatctgc tatgaacaga ttaggaggac gtgtagtagg cttttcagag ccaaattcat
    13381 cctctgcatc aaaaggtgaa actctaggag atacaatgag gatagtatct ggttatgttg
    13441 atataatagc tatgagacat cctcaatcag gtgcagcttc agaagcagct agatatacgg
    13501 aagtgccatt tataaatgca ggagatggca aaaaccagca tccaactcaa acacttacag
    13561 acttacttac tataaaatca ttaaaaggaa ctttagaaag ccatacaata ggattatgtg
    13621 gtgatttaaa atatggaaga actgtgcatt ctttggttaa ggctatggca agatataaaa
    13681 atacaaagtt tgtatttata gcacctgaag agttaaaaat gccagattat ataaaagaag
    13741 ctataaaggg acatgcatat tatgaaacta ataatttgga tgatgttata ggaagcttgg
    13801 atgttttata tatgactaga gttcaacaag aaagatttga agataaatca gagtatgaaa
    13861 gacttaaaaa ctattatatt ttaaataaag ctaaattaga aaaggcgtct aaagatatgt
    13921 tagtaatgca tccattacca agagtaaatg agatagacat agatgtagat agtgatgata
    13981 gagctgtata ttttaaacaa gctaaatatg gaatgtatgt tagaatggct ttaataataa
    14041 aacttttagg tataaatgaa gactaatagt tatcatataa tagtaaaaca aaggagattt
    14101 tgggatgtat aaaattctgg aaaatatata tataggcgaa gatatgtata gaatgaaagt
    14161 aaaaggtaat tttgaaggta aaatgggaca attctatatg ttaagagcat gggatactta
    14221 tccagtactt tcaagaccta taagtataca tgatatagat gaagaaggga taacttttct
    14281 ttataaagta gtaggagaag gaactcaaat tttgagtaac ctaaaggtaa atgatactat
    14341 aaaattggaa ggcccttatg gtaatggata tgctaaggtt gatggaaagg tagctctagt
    14401 aggaggagga atcggtgtag ctccactata tttggttgct aaaaatataa aaaattgtga
    14461 tgcatatctt ggctttagag aagatgttat attagaagat gaatataaac aagtatgtaa
    14521 taaagtatat acaacagtag gaaatacttt tgtaacagat ataattgatg ttgagaaata
    14581 tgattatata ttaacatgtg gtccaactcc aatgatggaa aaattagtta agatggtaga
    14641 aggtactaaa acgagaatta tggtatctct tgaaaaccat atggcttgtg gagtaggtgc
    14701 ctgtttagta tgtacgtgta aaactaatgg aggaaataaa aagacttgta aagatggtcc
    14761 agtgttctgg ggagaggacg tgatttttaa tgggtaattt aagtgtgaaa tttggaagtg
    14821 tagaatttaa aaatccagta ataatggcgt ctggaacttt tggatttgga aaagaatata
    14881 atgagattta tgatatacaa aaacttggtg gtataagtag caagggtctt actttaaata
    14941 aaaaacctgg taacaatggt atgagagttc atgagacacc atccggtatg atgaatagtg
    15001 ttgggcttga aaaccctgga gttcaaggat ttatagacta tgaattacct ttttttagta
    15061 aactagactt agtcagaata gctaatgttg gaggtgggac attagaagac tacttattag
    15121 gggttcaaat gttaaatgac aagcctatag acattattga attaaatata tcttgcccaa
    15181 atgtcaaagc tggtggaatg gcttttggta taaaaaatga agtggcaaga gaagttgttc
    15241 gtgaggtaag aaacataacg aaactgcctt tagtaataaa attatcaccc aatgcagaag
    15301 atattgtagg aatggcaaaa gtttgtgaag aagaaggtgc tgatggagtt tcattagtta
    15361 atacttttaa agcaatggca attgatataa aaaatagaag accagtattt gaaaatgtat
    15421 atgctgggtt atctggacca gctataaaac ctatagcttt gagaatggta catgaagtgt
    15481 gtaaaaatgt aaatatacct gtaatgggaa tgggcggaat aactaaagcc acagatgcta
    15541 tagagtttat aatggcagga gctacttgta tccaagttgg aactgcaaat tttatgaatc
    15601 ctagaatagg tattgaaata atagatggaa ttaatgaatt tatggataga gaaggaataa
    15661 aaagtttaga cgaaataaga ggaattatat aaggtagaat aaatagtatt ttaaagataa
    15721 aaaatataga ttaaataaac aataatttta taacattaac atggaggaaa aataatatga
    15781 gtaaagtaga tgtagtggat atattaaaga aaagtgatgc attgttggag ggacattttt
    15841 tattatcttc aggaaaacac agtaatagat acgtacaatg tgcaaaagta ttaagatttc
    15901 cagaatatgc tgctgaggta ttaagtacag ttgttgaaca aataaaagac ttagatatag
    15961 acttagtagt aggaccagct atgggtggag taatagtttc ttatgagtta ggaagacaat
    16021 taggaaaaga agctgtattt actgagagaa aagacaatac aatggagtta agaagaggat
    16081 ttgaagttaa aaaaggagca aagataataa ttgctgaaga cgttgtaact actggtaaat
    16141 caactatgga gacaaaaaga gtattagaag ccttaggtgg agaagttgta ggtgttgcat
    16201 gtatagcaga tagaactaat catgatatag gtatgcctat atatagtgct ataaaacttg
    16261 atattcaagt ttatgaatct gatgagtgtc ctttatgtaa ggaaggaaaa ttaccagttg
    16321 ttaagcctgg aagtagggag ttcaaggaat tagggatgta ataaaaactt aattatttat
    16381 agtgttactt aaaaaatgta aattttttag agtaaaaatt tgtaatataa catataactt
    16441 aaaaataaaa agcagttagc cttataaatg aaggttagct gttttttttg ttaaaaatat
    16501 atttacaaaa taagagatag tagcagtaca atataagtat aaggtaaaag tattatatat
    16561 tagggagggg taagtatgaa aaatcaatta aatgaaaatc ataaaaaggt tgtatttttt
    16621 ggagtaggtg ctgtaggagc tacttttgca gaacaatttt ttaactctaa atatgatttt
    16681 aaaattcttt gtgataatga aagaaagaaa agatatttag aagaaggatt tataataaat
    16741 ggaaaaaggt atgattttga ttatgtaact aaagatgagt ataaacaaga ggctgatttt
    16801 ataattatag gtctaaaata taataattta aaagaaaata taaaagaatt agatggatta
    16861 gttggaaaaa atacagttat aatgtctctg ctaaatggag ttgatagcga agagataata
    16921 ggagaaagat ttggaattga aaaaatggta tattcatatg ttaccaatat agatgcaaag
    16981 aaaatcaata ataatattat acacactact aatgggataa ttgtatttgg taacaaagat
    17041 aatagtgaag atagaaaaac taatataata accgaagtct ttgatgatgt aaatatagaa
    17101 tatactttat caaaagatat tcaacgagat atgtggtgga agtacatggt taatattggt
    17161 gtaaatcaaa cttcagctat acttggtgca ccttatggag tttttcagag ttctgagcat
    17221 ttaagagaat tagcaaaatc tgcaatgagg gaagttgttg ctatagcaca agcaaaagac
    17281 atatctctta cagaagatga tgtggaacat tcattacata gaatactaga acattcaaaa
    17341 gaaggaagaa catcaatgct tcaagatgtg gaagctcata gacttacaga agtagatatg
    17401 ttttctaaga atatctgcaa acttggaaaa aaatataaca tacctactcc tataaatcag
    17461 actttctttt atatgataaa agtaattgaa agtagatttt aaagggtctt tgtaagaaat
    17521 attgataatt attgatttta aattttatta atgttatata ctatttaagt tactaaatat
    17581 ttttaactct tcaatgaaaa tagttgctgc ttttgataaa gttatatctt ttggaaatat
    17641 atatgagaaa tgtctgttgt agctgcttcc aagctctatg acagacagct cattgtttag
    17701 aacaggtaag cttgtcacta gattagagac tattgtaatt cctaaattat ttctaacaga
    17761 ttctttgaca gcataattac ttccaaaaac catcatactt tttggaataa tttctttaac
    17821 acttaaaaac atatctaaat aatctctggt tccagaacca tcctccctga ctatccattt
    17881 ttggttttga agtttatcaa aactaaaatc ttttaataaa tgactcttgt atggaagtgc
    17941 taaaaccatc ttatcttcaa aaaaatattc ttgaataaat gaaggggaag agcaagtgcc
    18001 ttcaataaga ccaatgtcta aaatataatc cttaacatga gaagatacga ttgatgtatt
    18061 ttttataaaa acttctacat ctatgtcagg atacttttta gaaaataatg ctagaaaatt
    18121 tggtaaaata tactctccaa tagttaaact tgcaccaatc tttaagtgac cagttatagc
    18181 atctgaaact tggcttacat catgataagt aatatttaaa agatttaata tttctttagc
    18241 tcttttgtac aagatttgtc cactttctgt tataaaaatg gttttttgtt taatagacct
    18301 gtttataagc acaactttaa aataattttc tagatttttt atgtgtttac ttacacttgg
    18361 ttgtgataga tttaaatatt ccccagcttt agtaaagttt ttgtattcaa caacagctac
    18421 aaatgttttt aattcttcaa acaatttttt gtctccttta cctaatgaaa ttttacattt
    18481 tttatatact ttctacagat aattatacta tatataataa ccattaaaaa cattaatcat
    18541 tacgatatta tatatttatt ttacttatgg ttattacaat tatatactat tgctatgaag
    18601 aaaactatat taattataag gtaggtatat tatgttaaag aaaaaaagca gtttgacatt
    18661 aaaaactata agggaaatat taccaggact gtttgtatca gtattagtag gttatattag
    18721 tatatttatt tctattttaa ttcctaaggt aggggcagca tcaatttcta tatttttagg
    18781 tatgtttgta ggaaacctat ttttaaatca aaaggtattt caaaaaggtt ataaattttc
    18841 agagacagat ttgttatcat attcaatagt actattagga gcaacactca gtgtatctac
    18901 cttaatagac ttaaaagttt caggaatttt atttataata ttgcaaatga caataacaat
    18961 aatagctgca ctttatatag gtaaaaaact aggatttgaa gaaaacttta gatttctaat
    19021 ggcaagtgga aatgcagtat gtggttcttc tgcaatagga gcaacagttc cagtggttaa
    19081 tgcgacagat aaagaaaagg gaattgcagt aactattgta aatgttactg gtatatttct
    19141 tatgttttta cttccaataa tatctcaatt tttatatagc catgaacttg taaaaacttc
    19201 agcaatgata ggtggaactt tgcaatcaat tggtcaggtt gtagcaagtg gagcaattgt
    19261 tggagaaaat gtaaaagatt tagctactat ttttaagata gtaagagtaa tatttctagt
    19321 tgttgttgta ttagtatttg gacatatgaa aaatcaatct aatagggaaa ttcttgaaga
    19381 agaaaaggat gagataaaaa agaaaaaagt gactatacca tggtatgtaa ttggattctt
    19441 tataacttgt gcactttttt caatgaacat tataccacaa gaagtatctg tgttgtgcaa
    19501 agaaataagt aacaaactag aaataatagc tttagcagca attggattaa aggttaatgt
    19561 aaaagattta gtgaaacaag ggaaagaagt ttctttatat ggtttatttg taggtatagt
    19621 acaagttgta tcagctgtag ttttaataaa aatatttata tagcataaaa atagtatttg
    19681 taatattaat ttgattaatt aaaataatat gatactaata atttataata taaataaaaa
    19741 atgaatttcg at
//
LOCUS       DAMJVI010000073        19191 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.075, whole genome shotgun sequence.
ACCESSION   DAMJVI010000073 DAMJVI010000000
VERSION     DAMJVI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.075"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            148..648
                     /locus_tag="PC545_002557"
     CDS             148..648
                     /locus_tag="PC545_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457321.1"
                     /translation="MIEGIKFIKAEEKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNIINKNLPQLFIIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSNLL
                     KQIIDLSKEYGYEKIELDVFKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(898..1677)
                     /locus_tag="PC545_002558"
     CDS             complement(898..1677)
                     /locus_tag="PC545_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457322.1"
                     /translation="MNKNILNNSFPEVPESFHNSLSKTLNSLPEREENYKMINNKTHK
                     LPFRKGLVATLAITLILSTTALAVGHIYSLVGKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNKKAFDKENNVLGKSKALSFEYKNGNDEISLDTANNVLGKS
                     SDNLKVVDTYKDINISYEAFAQKFVPEGYKMTEQDKIDEKSGKYTFSVGPNNESDKVE
                     VNKFKFLTWKQDGVYYSFTVQDSNLSMNDLVKMAHEVIDAK"
     gene            complement(1674..2153)
                     /locus_tag="PC545_002559"
     CDS             complement(1674..2153)
                     /locus_tag="PC545_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2457323.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKSLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            3139..4146
                     /locus_tag="PC545_002560"
     CDS             3139..4146
                     /locus_tag="PC545_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDF2457324.1"
                     /translation="MGKYQDSKKVVRNYFEALENATGDEVENVLKQHMKSDYNWKGVY
                     PFREQEGTENVADVFWKPLKKSLSNMQRRQDIFIAGTNEIDNSEWVMSMGHFMGLFDN
                     DWLGIKHTRKMISLRYAEFNCVQDGKIAKTGLFVDIIGFMIQAGVNPLPPQTGSYFVY
                     PGPIDHNGLLFEDADEVEGVKTLALVNKMVDDLSELNESGAMGCPPEILEKSWAKDMI
                     WYGPGGIGASYTIPRYQEQHQLPFRNNLKGKTFNGHVCRFAEGNFACFFGWPNLTNVP
                     CGGFLGLPGGEVKADMQVVDVYYRKGDKLQENWVLIDIPYWLKQQGLDILERTKSIFN
                     A"
     gene            4184..5611
                     /locus_tag="PC545_002561"
     CDS             4184..5611
                     /locus_tag="PC545_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HDF2457325.1"
                     /translation="MASTNKNNATSKEEEKEVSTSGLESCTVKENKAKMNQTNFSNIN
                     ENQTNENKTNTYKRNIDKGNIDKGNIDKTNTNKSDINKVNIDTVDTDKLNTNTLTKKN
                     KKVIFKGEDNMSSTKIYYGDSLEVTPVGVMDYNDFSKQTKREQEIPGFDSKYRDFVDY
                     IMKITHNIWEEKGIGVIYDTYHNNVTMHCGSSNLVGIKDVISNTLQTLHAFPDRRLIG
                     QNVIWSNFGADGFLSSHRVLSTATNLGDSNFGPATGRKINFRTVIDCATTNNRIHEEW
                     LVRDNLWIVTQLGLNPQEVAKSMAKASESKVLSLQSTYGICESMDGQFMPTKYQAKDD
                     SAGEMMLEMTSRIYNYKYINEVKKYYHDNAVVHFICDKDLNGYDEIQGMIVSLLASIP
                     NGSYEVERVTCNQREKNEGYDVSVRWRLRGINEGIGFFGQPSGKHIEVMGINHYHILQ
                     GKVKEEWITFDGMDVLKQMYMGVEE"
     gene            5753..7018
                     /locus_tag="PC545_002562"
     CDS             5753..7018
                     /locus_tag="PC545_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457326.1"
                     /translation="MKTTVEIENDIILGKDYSNLKRGIAFFSIALIYFFYCYNFMVGT
                     FIKPTMIYALADGGFGFSLKQTEEIFAVMSFGTIPGTFIFGVISTKIGKKRTLISVAL
                     LIGLTTFIPMLSPTNIMLWKIARLCTGVVLGGVFGTAMPLVADMFPSKYRGKLAAILT
                     ALFSLAMIFGGKVYGVLGDANWQILMYTAIIPPIVGAILAIFFVPDDLEYTKELVKKG
                     KETGEKISYISMYKGKYLWIGLGTILLSGANFTAYSAFSNNATTYLVTRIGMSAAVAG
                     SIYSLQGIGQLVGYLFWGSIADKFGRKIPFIGMALAAVFVFIYTKLGGDNTTTFYMIS
                     VLLGVAVGYSGAWGAYYTELFPSKYRSLSSGMSFNGGRIISTFAIPAIAGTASGTLGM
                     MTIFYISIAVFVAGAIVWLFLPETLNNKK"
     gene            7060..8658
                     /locus_tag="PC545_002563"
     CDS             7060..8658
                     /locus_tag="PC545_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HDF2457327.1"
                     /translation="MISSIDVCIIIGYLLLMLVIGYYSGKDNKNQEDYFLAGRSMPWI
                     PIALSVAATMISANGFIGGPGWAYVDGMYPVMVNITVPLAIFFALSITTPVIYKLKIT
                     SIYEYMGLRLGNYSRGLTIIQFFINSLIQASSMVYIPVLIIQMITGWSLYALVPIVVL
                     VSIIYTLLGGIKAVIWTDSIQMIVVISAVFIVIIVALKGMNLSFFDTLQIAQQSGKLN
                     TFDFSRDISVTNTFWATLIGGTVMWIRYFCFDQSQVQRVLTSKSLKCAKNSFVVSAFV
                     MNLVYYFMLLVGVILFVFYGGRTFETSNEIMITFILNELPVGAIGIIIAGVFAAAMSS
                     IDSILNSMTTVFTKDIYEFYFKKDNKESSLKVTMIITVVIGVVMTGFIIMGFGGSIKS
                     ILDLVGNYISYFAGPATGAFVLAMFTYKANDKGVSIGFIVGLVLGFFISNKYNVSWLW
                     NPAIGATITLIFGYIFSVILKKNHNADDIKKYTAFGMRNHLISQGMDKVDGVSIVPFS
                     LGKQELIVLAFFIIQYIILFLIQF"
     gene            8693..9715
                     /locus_tag="PC545_002564"
     CDS             8693..9715
                     /locus_tag="PC545_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2457328.1"
                     /translation="MNQKKVHYNKSATSKDVARLAGVSQSSVSRAFGSANGKGVKPEV
                     RERIFRVANEIGYVPNLVARGMISGKTNVIGLIVGDSLGPFYNRIINLFVEKIQEIGK
                     QCLVFKVPRQQSIDSIISKVIQFQVEAVIITASAMNKVMAETCEENNIPVILFNRFIP
                     GIKISTVYVDPIEGGGMAAEYLLKKGHKNIGYIQFTRETSEEMEKKIGFYSKLRQSGI
                     HNLKEESANYDYDSGYEAGKRMLALSNPPTAIFCTSDLIAIGVMDAARFEYKLRVPED
                     LSVIGYDDIQMASWKSYNLTTIRQPLDLLIEKTINILKSLLNEENPESVVEMLKPELI
                     ERGSTL"
     gene            complement(10198..11601)
                     /locus_tag="PC545_002565"
     CDS             complement(10198..11601)
                     /locus_tag="PC545_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2457329.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEICYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     gene            12468..12977
                     /locus_tag="PC545_002566"
     CDS             12468..12977
                     /locus_tag="PC545_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDF2457330.1"
                     /translation="MSKELAINEQIKDKEIRVLSPTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            13005..13199
                     /gene="rpmI"
                     /locus_tag="PC545_002567"
     CDS             13005..13199
                     /gene="rpmI"
                     /locus_tag="PC545_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDF2457331.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAFKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            13255..13611
                     /gene="rplT"
                     /locus_tag="PC545_002568"
     CDS             13255..13611
                     /gene="rplT"
                     /locus_tag="PC545_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDF2457332.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGVEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            13921..14466
                     /locus_tag="PC545_002569"
     CDS             13921..14466
                     /locus_tag="PC545_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457333.1"
                     /translation="MNEKLMDCFVNPIKCKLLIEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSGFSVCPVYASIDEIVEVSKKIASILEPLRNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            14453..14626
                     /locus_tag="PC545_002570"
     CDS             14453..14626
                     /locus_tag="PC545_002570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457334.1"
                     /translation="MKGNKSDLHGVEPIYTENRFMIYICIQSKVSLLGLYFIVPFHPM
                     TWSGTMKFFNFSY"
     gene            14901..16826
                     /locus_tag="PC545_002571"
     CDS             14901..16826
                     /locus_tag="PC545_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2457335.1"
                     /translation="MKIKFVIMPLVIFLMVLFVGCQSTKDTEVKAKDTEIKILGTGDL
                     HSILTDSMVSYINEEREKNKNLLMVDAGDFYGTQSREMWEWSSGKKLINIKRNGRAEY
                     DDIIKSSKDEVPIVKDMAKLKYDAVVLGDNEFISNDKQSLDKLVSDFKNNNMPLVSAN
                     IYEQSGENYVQPYVMKNIKTDEGNVKVGILGLTIKEVGETLGLEDFEKDKKARELGEQ
                     ADYKGKLYANDLVEDAKKWIKVMEKESPDIIVAVVHSGEEPKTDRNPGNRIKELATTV
                     DGIDAIVAGHTHEKIDEHKYKNKSGDEVIVTQPGWHGDRVSEINFKLNKENGKWDIKE
                     KSSKLKIIDKEVSIVATADLHSHFSDELTVDLVNERSNPIEPVVVDAGDFLDPQTDEM
                     TQWYKEWKSIRDNNSDKTISRCPMVMDMNQGMYDAVVLGNHEFVSEKDEFWSDERLLD
                     AVIEDFEQVAISVLSANIYRQSGKNYVKPYIIKDVETSEGNVKVGILGLTIKEVGKGL
                     KNVNLQEQFQYKDKLYANDLVEDAKKWVKVMKKKKPDIIVAVVHSGEEPKNPKHPGNR
                     IKELATTVDGIDAIVASHTHEKIDEHEYKNKSGEKVIVTQPGEHGKYYSKITFKVNKE
                     KGSWTINDKFSKTIEVE"
     gene            17227..19131
                     /locus_tag="PC545_002572"
     CDS             17227..19131
                     /locus_tag="PC545_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2457336.1"
                     /translation="MKIKFIIVASIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LISYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLVRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNNIPLVSANVYEQSGEN
                     YVKPYVMKNINTDKGTVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVNVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKV
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNTKEMTKWAKEW
                     QYNENKGIYKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEEDLSNSSLSKDSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
BASE COUNT         7250 a         2137 c         3414 g         6390 t
ORIGIN      
        1 tacaagagat agatatattg gttttaattt tatagaaata tacaaagata ttttaagcta
       61 atatatagta atttatatag cattataaaa tagaatcata taaaataagt taactacaat
      121 caaaaattaa aaaggggaaa tgttaagttg atagaaggca taaaatttat aaaggcagaa
      181 gaaaagtata tttattcgta ttggcaaact tttgataaaa tagcaaaaga gaggaaatat
      241 ctagctatgg atgaagcctt tccttttgaa gaaacagttg aatttataaa aaatattata
      301 aataaaaact taccacaact gtttataatt gatttagaat cagataactg tattggttgg
      361 tgtgacgttt tacctaaaac tgaaaaagtt ggttatttag gcatgggtat tcttaaagag
      421 tatagggaaa aaggtatcgg aagtaatctt ttaaaacaaa ttattgattt atcaaaagaa
      481 tatgggtatg aaaaaattga gcttgatgta tttaaaagta atagtagagc tattcatgtt
      541 tataaatctt tagggtttgt agaggtaaat acaatatcta gtggttttac atggaatgat
      601 agacctgtaa aagaagaagt aattcaaatg gagcttacac ttatataaaa taaagttaaa
      661 gtctttaaaa ttccatatac acttgtataa aataaagtta aagcccttaa aattccatat
      721 aaaagaaata tttttaaggg ctttttaata atgcttcttt tgaaggtagc taaatatcta
      781 aaattttata tattaaattt aaagaaaata taaagatgga agatacaacc atgtttacta
      841 ataaaaaaat taaagtcata aataataaac tcataaacaa cttattatta cttatgttta
      901 ttttgcatct attacttcat gagccatttt aactaagtca ttcatagata aatttgagtc
      961 ttgaactgta aaagaatagt aaactccatc ttgtttccat gtcaaaaact taaatttatt
     1021 aacttcaact ttgtctgatt cattatttgg ccctacacta aatgtgtatt tgcctgattt
     1081 ttcatcaatt ttatcctgtt ctgtcatttt ataaccttct ggtacaaact tttgtgcgaa
     1141 tgcttcataa gaaatattaa tatctttgta agtatctaca acttttagat tgtcagaact
     1201 cttcccaagt acattatttg ctgtgtcaag agatatttca tcattgccat ttttatattc
     1261 aaaactaagt gcttttgatt ttcctaaaac attattttct ttgtcaaatg cttttttatt
     1321 tacagtatat gctcctttaa atttatatcc attactaaat tcagaaacta tattaggggt
     1381 aaagttaaag tcttttttta cttgttctgt agttgggata gatgtatagg tagccttagt
     1441 acttgatttt ccaaccaaac tatatatatg tcctactgca agggctgttg tgctcaatat
     1501 caaagtaatg gctaaagttg caactaagcc ttttctaaat ggtaatttgt gtgttttatt
     1561 attaatcatt ttataatttt cctctctttc tggtaaacta tttagtgttt tgcttaaact
     1621 attatgaaaa ctttctggaa cttctggaaa cgaattattt aatatatttt tattcatatg
     1681 attacctccg tgttttctaa tttagttttt aataatcttc ttcctcttga taatctactt
     1741 tttacagttc cttgaggtat atctaatatt tccttaactt catctactga ataaccttca
     1801 atatagtaaa ggactattgg aattttaatt tttttaggta gactttgaat agcagtatac
     1861 aaatcgctat agtcatcttt ttcatcaatt tttttgtttt tgaagacatc ttcaaatgat
     1921 agagatttta atcgtttgcg tcttaatgaa ttacattcat ttatgactat tctaatcagc
     1981 catgttttaa agtattgttc ttccttaaga gaatccaact tttcatatgc ttttaaaata
     2041 gcattgttga cggcatcttc acagtcttgg tcgtttccta gaatagactt agatactcga
     2101 tacaaagtct gttctgattc aagtatattg tttgtaaatg tagttttatc caactaagtt
     2161 ccttcccttc tatgcaaatc gtagcgctac ttttttgtct ttcacttatt agataaaatt
     2221 ttactataga tggttcactt tttttatgat tatttgataa atttaagttt gaaagttttg
     2281 cctattctat acaaatattc tataatatat tttttctagt attttcccct catatataaa
     2341 gccatgtaga tatattaaac tttagaagcc atttatatat ttagttttat agtaagtctt
     2401 tatattgagt aattagaaaa tagtaagtat ttaatgttga gtagttagaa taacaatgct
     2461 tttggattat ttacttcatg tacagattta aaataagttc tttaaatagc aactttttga
     2521 aaaaacgcaa aaatatttta tgttgtctga ttatatttat agatgtgaaa caatattgta
     2581 tagagtaaaa gtatatagaa gatgattaca tagagtttat acttgaattt tacaaatata
     2641 attatcaaaa tactgtcaaa gagaaaaata gttttgatga acgtttttat caaattgcta
     2701 cagagacagg gctaagtgct aatactgtat ataaagtatt caataaaata ataaatagtg
     2761 gaaaaactga atttacatca aaggaattag ctactatatg tggtgtaagt attagaacta
     2821 tggacaggat tatacttaga ttgtgtgatt ctggttattg tgaggtagtg agtgaacagc
     2881 ttatgagcaa gtatggtaga cccagtagaa tattacgttt taaacctttt ctgttgtttt
     2941 aaatagatat aagtcaaaaa ttaaattttt atgataacat tttctttaca attttttaga
     3001 tatatgttaa tatttagaaa aatgcatatg tattcataaa ataaacttaa aacagctaaa
     3061 aaatcattaa aaaggaaatt gtatgcatga aatgagaaag ttaatttata acttaaataa
     3121 atctgggagg agataattat gggaaaatat caggattcaa aaaaagttgt aagaaattat
     3181 tttgaagcat tagaaaatgc tactggggat gaggttgaaa atgtcttaaa gcaacatatg
     3241 aaatcagatt ataactggaa aggtgtgtat ccatttcgtg aacaagaagg cactgaaaat
     3301 gtagcagatg tattttggaa gcctttaaaa aaatcattga gtaatatgca aagacgacaa
     3361 gatattttca ttgcaggaac aaatgagatt gacaatagtg aatgggtaat gagtatgggg
     3421 cattttatgg gactttttga taacgattgg cttggcatta aacatactcg taaaatgatt
     3481 agtttaagat atgcagaatt taactgtgtt caagatggta aaattgctaa aactggatta
     3541 tttgtagata taattgggtt tatgattcaa gcaggagtta atccattgcc accacaaaca
     3601 ggttcatatt ttgtatatcc agggccaatt gaccataatg gattactttt tgaagatgca
     3661 gatgaagttg aaggagtaaa aactttagca ttagtaaata aaatggtaga tgatttatct
     3721 gaattaaatg aaagtggtgc tatgggatgc ccaccagaaa tacttgaaaa atcttgggct
     3781 aaagatatga tttggtatgg tccaggtgga attggtgctt cttatactat acctagatat
     3841 caagagcaac accagctacc atttagaaat aatttaaaag gaaagacttt caatgggcat
     3901 gtatgtagat ttgcagaagg taattttgct tgtttctttg gatggcctaa tctgactaat
     3961 gtaccatgtg gaggtttttt aggattacca ggtggagagg ttaaagcaga tatgcaagtt
     4021 gttgatgttt attatcgtaa gggcgataaa cttcaagaaa actgggtgtt aattgatatt
     4081 ccttattggt tgaaacaaca agggcttgat attcttgaga gaactaaaag tatttttaat
     4141 gcttagttat gcatacgtat tctaaaataa agggggatat aaagtggcat ctaccaacaa
     4201 aaataatgca acaagtaaag aagaagaaaa agaagtttct acaagtgggc ttgaatcatg
     4261 tacggttaaa gaaaacaaag ctaaaatgaa tcaaactaat tttagtaata taaatgaaaa
     4321 tcaaacaaat gagaataaaa cgaacacata taaaagaaat atagataaag gaaatataga
     4381 taaagggaat atagataaaa caaacacaaa taaatccgac ataaataaag ttaacataga
     4441 tacagtggat acagataaat taaatacaaa tactcttact aaaaaaaata aaaaagttat
     4501 atttaagggg gaagataata tgtcatctac aaaaatttac tatggagatt cattagaagt
     4561 aacgcctgtt ggagtaatgg attacaatga tttttcaaaa cagacaaaaa gggaacagga
     4621 aatacctgga tttgattcta agtatagaga ttttgtagat tatattatga agattacaca
     4681 taatatctgg gaagaaaaag gtataggtgt tatttatgat acatatcaca ataatgttac
     4741 aatgcattgt ggctcttcaa atttagttgg tattaaagat gttatttcca atactcttca
     4801 aacattacat gcgtttcctg atagaagatt aattggtcaa aatgtaatat ggtctaactt
     4861 tggtgctgat ggttttttat cttctcaccg tgttttgtct acagcaacta acttaggtga
     4921 tagtaatttt ggaccagcaa caggtagaaa aataaacttt agaactgtaa ttgattgtgc
     4981 cacaactaac aatagaatcc acgaagaatg gttggttaga gataatttat ggattgttac
     5041 tcaattagga ttaaaccctc aggaagtagc aaagagtatg gcaaaggcaa gtgaatctaa
     5101 agtgcttagt ttacaatcaa cttatggtat ctgtgaatct atggatggtc aatttatgcc
     5161 aactaagtat caagcaaaag atgattctgc tggcgaaatg atgcttgaaa tgacaagccg
     5221 tatttataat tataaatata taaatgaagt taaaaaatac tatcatgata atgcagtagt
     5281 acattttata tgtgataaag atttaaatgg ttatgatgag attcaaggaa tgattgtaag
     5341 tttacttgca tcaattccaa atggaagtta tgaagtggaa agagtaactt gtaatcaaag
     5401 agaaaaaaat gaaggttatg atgtatctgt aagatggaga ttacgtggaa taaatgaagg
     5461 tataggattt tttggacagc cttctggaaa gcatatagaa gtaatgggaa tcaatcatta
     5521 tcatatacta caaggtaaag taaaggagga gtggataact tttgatggta tggatgtatt
     5581 aaaacaaatg tatatgggag tagaagaata aagctgttta gagtataaat agttttgaaa
     5641 tatataggga agtattgctt tataaaattt aagtggggaa tgtatatgaa aaatagatgt
     5701 ttaaactttt agcaaaatta tgactaaatt taaaaggggg aattataaaa atatgaaaac
     5761 aacggttgaa atagaaaatg atatcatatt aggaaaagac tattcaaacc taaaaagagg
     5821 aattgcattt ttttcaattg ctttaatata ctttttttat tgctacaatt ttatggtggg
     5881 tacttttatt aaaccaacaa tgatttatgc tttagcagat gggggatttg gtttttcatt
     5941 aaaacaaaca gaagaaatat ttgcagttat gtcttttggt acaattccag gtacatttat
     6001 ttttggggtg atttctacaa aaattgggaa gaagaggact ttaattagcg ttgctttact
     6061 gattggattg acaacattta taccaatgtt atctcctaca aatattatgt tatggaaaat
     6121 agcacgtttg tgtacaggag tagtgttggg aggcgtattt ggaacagcta tgcctttagt
     6181 tgcagatatg tttccaagta aatatcgtgg taagttagct gctatattaa ctgctttatt
     6241 ttcattagca atgatttttg gtgggaaagt atatggcgtt ttaggtgatg caaattggca
     6301 aatattgatg tatactgcga ttataccacc tatagttgga gctatcttag caattttctt
     6361 tgtgccagat gatttggagt atacaaagga attagttaaa aagggaaaag aaactggtga
     6421 aaagattagc tatataagta tgtataaggg aaaatactta tggattggat tgggaacgat
     6481 tcttttatca ggtgcaaatt ttacagcata ttcagcattt tcaaataatg caactacata
     6541 cctagtgaca agaataggaa tgtcagcagc agtggctggg tctatatata gtttacaagg
     6601 aattggtcaa ttagtaggat atttattctg gggttcaatt gcagataaat ttggaagaaa
     6661 aatacctttt ataggtatgg cattagcagc agtatttgtt tttatatata caaaattagg
     6721 tggagataat acaacaacat tctatatgat atcagtatta ctaggtgttg ctgttggata
     6781 ttctggagca tggggagcat actacacaga gttgttccca agtaaatatc gttcgttgtc
     6841 atctggtatg tcatttaatg gaggaaggat tatatctacg tttgcaatac ctgctatagc
     6901 tggtacagct tctggtactt taggtatgat gactatcttt tacatatcaa tcgcagtctt
     6961 tgtagcaggt gctattgtat ggttattctt accggagacg ttaaataata aaaaataaaa
     7021 tactaggaat ttattttgtt acttagaaag gggtggctta tgatttcatc aattgatgta
     7081 tgtattataa taggatattt gctattaatg ttagttattg gttattattc aggaaaagat
     7141 aataaaaatc aagaagatta tttcttggca ggtaggtcaa tgccatggat tccaattgct
     7201 ttatcagttg cagctactat gattagtgca aatggcttta taggtggtcc tggatgggca
     7261 tatgtagatg gaatgtatcc agtaatggtt aatattacag tacctttagc aatatttttt
     7321 gcactgagca ttaccacccc agttatctat aaattaaaaa taacctctat atatgaatat
     7381 atgggacttc gtttaggaaa ttattcaaga ggattaacta ttattcaatt ttttattaat
     7441 tcattgattc aagctagttc tatggtatat attccagtgc ttattattca aatgattact
     7501 ggctggtcac tttatgcttt agtcccaata gttgtacttg tatcaattat atatactcta
     7561 ttaggtggga ttaaggcagt tatatggact gatagtattc aaatgatagt agtaataagt
     7621 gctgtattta tagtaattat tgtagcctta aaaggaatga atctaagttt ctttgataca
     7681 ttacaaattg cacaacagag tggaaaatta aatacatttg atttcagtag ggatatttca
     7741 gtgacaaata ctttttgggc tactttaatt ggtggaactg taatgtggat aagatacttt
     7801 tgttttgacc aatctcaagt acaaagagtt ttaacatcta aatctttaaa atgtgcaaaa
     7861 aattcttttg tagttagtgc atttgttatg aatttagttt actactttat gctattggta
     7921 ggagtaattt tatttgtttt ttatggggga aggacatttg aaacttcaaa tgaaataatg
     7981 attactttta ttttaaatga acttccagta ggagccattg gaattattat tgcaggtgtg
     8041 tttgcagcag caatgtctag tatcgactct atattaaaca gtatgacaac tgtttttacg
     8101 aaggatattt atgagtttta tttcaaaaaa gacaacaaag aatcatcatt aaaagttaca
     8161 atgattataa ctgtagttat aggagttgtt atgacaggtt ttattattat gggatttgga
     8221 ggaagcatca aatcaattct tgaccttgtt ggtaactata tctcatactt tgctggacca
     8281 gctacaggtg catttgtact agcaatgttt acatataaag ctaatgataa aggagtatct
     8341 attggtttta ttgtagggtt agttttagga ttttttattt ctaataaata taatgtgagt
     8401 tggttgtgga atccagcaat aggtgctaca ataacattga tatttggata tatttttagt
     8461 gtcattttaa agaaaaatca taatgcagat gatattaaaa aatacactgc atttggtatg
     8521 agaaatcacc ttatttctca aggaatggat aaagtagatg gagtatctat agtgccattt
     8581 tctttaggta aacaagaact aattgtatta gcttttttta ttatccaata tattatatta
     8641 tttttgatac aattttaaga agaaatacta gatatttaag gagaaatact aaatgaatca
     8701 aaagaaagtt cattataata aatcggctac ctcaaaagat gttgcaagat tggcaggagt
     8761 atctcaatca tctgtttcaa gagcatttgg tagtgcaaat ggaaagggcg taaaacctga
     8821 agtaagagaa agaatttttc gtgtagcaaa cgaaatagga tatgttccta atttggtagc
     8881 acggggaatg attagtggaa aaactaatgt tataggattg atagttggtg atagtctagg
     8941 tccattttac aatagaatta tcaatttatt tgtagaaaaa attcaagaga taggtaaaca
     9001 gtgtctcgtt tttaaagttc ctagacaaca aagtatagat agtataattt caaaagtaat
     9061 tcagtttcaa gtagaagctg tgattattac agcttcggcc atgaataagg tgatggctga
     9121 gacttgtgaa gaaaacaata ttcctgtgat tctttttaat cgttttatac ctggtataaa
     9181 aataagtaca gtctatgtag acccaattga aggtggggga atggctgctg aatatctgct
     9241 taagaaaggt cataagaata taggatatat tcaatttacg agagaaacaa gtgaagaaat
     9301 ggaaaagaaa attggatttt attctaagtt aagacaaagt gggattcata atcttaaaga
     9361 agaatctgca aactatgatt atgattctgg atatgaagca ggtaagagaa tgttagcatt
     9421 atcaaatcct ccaacagcaa ttttttgcac aagtgacttg atagctattg gtgtaatgga
     9481 tgctgcaagg tttgagtaca agttgagggt gccagaggat ttatcagtga taggatatga
     9541 tgatattcaa atggcttcat ggaagagtta taatttaacg acgattagac agccactaga
     9601 tttgttgatt gaaaaaacaa ttaacatact taagtcctta ttaaatgaag aaaatccaga
     9661 gtcagtcgtt gaaatgttaa aacctgaatt gattgaaaga ggttctacac tttaagtggt
     9721 agatatttaa ttaagtgata gatatttaat taagtggtag atatttaatt aagtggtaga
     9781 tatttaatta agtggtagat atttaattaa gtggtagata tttaattaag tagtagatac
     9841 ctaattaagt tgtagatatc tgataagaaa tttttaagtt ttcaatcgtt ctaaattgtg
     9901 agtataaaaa tctactaaat ttagttatgt aaaatataaa ttataggtgt agaaactagg
     9961 ctactagctt ttataccact tcaaacatta ctggaggtag ttaagtttac agcagtttag
    10021 ttttatgtag atataatatt ctgtaaatag acaaaattaa aatatgtaag tgtctcaaaa
    10081 tgaaattttt agtttatgag gcactttttt gtataaaatc aaatgtattt ttataacttt
    10141 aacaataaaa agaggcttat ctctattttg agacagcctc aaatttttaa aactatatta
    10201 gtttacagct tcttttccta tcaaatcttt taactcttca tgagtcatag tttctttttc
    10261 aaataagaat ttagaaacag aatgtaaatc ttcaagatta tcctttaaca tctctaaagt
    10321 ctctttataa caaatttcaa ctatacgatt tgcttccttt tgaaccttat cacataagaa
    10381 agtcttatca tttccatcaa tagtcataaa tcctaagtta ctcataccat attcacaaac
    10441 catttggtta gctatttcag tgactttctt taagtcgtcc tttgcaccag tagatttatg
    10501 attaaatatt aattcttctg aagcctttcc agcaagtaat atttttattt ttccaatcaa
    10561 ctcatcctta gtataaatat atctatcttc atcaggtaat tgaagaacat aacctaatgc
    10621 ttgtcctcta ggaactatag atattttttg tataggacaa attcctacta tatcacttac
    10681 aagagcatgt ccagcttcat gatatgctac tatttttttc tctttttcaa ccaatgcaga
    10741 gtttttagat tctagccctg caataactct ttcaagtgcc ttgtcaaaat gttctgatgt
    10801 aatacactca ctgtcatctc taactgcaaa tatagctgct tcatttgcta tatttgaaag
    10861 atgagcccca ttgaaaccat gagttttttt agctaaaagt tctagattta cagatttgtc
    10921 tataggtttg tcatctgtat gtacctttag aatttcaaat ctagtgtgat agtttggtgc
    10981 acctatatga atgtgtctgt caaatcttcc tggtctaagt aaagcttcat ctaataagtc
    11041 aagtctattg gtagcaccta ttattaatac attggaatct ttattaaatc catccatttc
    11101 aactaaaagt tggtttagtg tttggtcttt ttcgttgtta ctttcaagat gtctctttgc
    11161 tcctacagca tctatttcgt ctataaatat tatacttgga gcttcttttc tagctttttc
    11221 aaatagagtt ctaactcttt ttgctcccac acctacatat ttttcaacga actctgaacc
    11281 agtaacatta aaaaatgatg agtttgtttc accagctaca gcagatgcaa gtagggtttt
    11341 acctgttcct ggaggaccat aaaataatac tcctttaggt atttttgcac ccattttttg
    11401 atatttttgt ggtgacttca taaaatcgac tatttcaaat agttcttctt ttacttcgtc
    11461 taatccagca acgtctctaa atgtagtctt tggttttgta gaaagtgaat catctgtttg
    11521 attatctctt tccatttttg tctgtgcaaa gacaggagct ggattgttga ttttcttaaa
    11581 aaagttgttt agcattttca tattcacacc tcaggtaatt aattattgtt aatcaatttt
    11641 atattttcca tatttactat tttttattat ttatttttca tggaaaaaat atacatgtta
    11701 tctaaataaa aataaatttg taagatgtat gaaaaaatga atttatgtat atagagattt
    11761 ctacttatgt attgaataaa gtattacaaa ttataaatta tgtattgatt atatattgag
    11821 taaagtatta taaagcatgt attgattaag tattgaataa aatattacaa atttagttga
    11881 aatgaaaatg ataaaaaatc ttgtactata ttttacaaat catataaata gagaaattgt
    11941 tgtagcacaa agtagtaggt agtatattag aatataatat aggatttgtt gtgtagtata
    12001 gtagcatatt agaatataat ataggatttg ttatgtagta tagtagtata ttagaataca
    12061 atatagaatt tgttgggtag tatagtgtta tattagaata caatatagga tttgttatgt
    12121 agaatatata gagtcatgta gaatataaat atataaaagg attttgggta taaatttaaa
    12181 aattaatagc aaaatgagta gtatagacat ataattagta gtaggaagta attaaatttt
    12241 tcctaaacaa aaaaaatagt tgcaaataat ttgattaagt gatatagtaa ataaggtaag
    12301 aataaaacaa agaagaagtc cgcttctcac cttacgccgg aggttagtaa ggtaaatata
    12361 agttaaagct taattttaat ttaagtttgt ttaaatttgg taacttatat tgcggatgat
    12421 tacgtcatcc gctttttatt ttataaaaat tttggaggtg tcctacaatt agcaaagaat
    12481 tagcaatcaa tgaacaaata aaagataagg aaataagagt cctttcacca acaggtgaac
    12541 aacttggagt aatgcctact aaagaagctc aagcaatggc taatagcaaa aacttagatt
    12601 tggttcaaat ctcgccgaat gcaaatccac cagtatgtaa gataatggat tatggaaagt
    12661 ttagatatga gcaagcgaga aaagagaaag aagctaagaa aaaacaaaaa actatagttg
    12721 taaaagaagt aagacttaga ccaggtattg aacaaaatga tttaaatacg aaagctaata
    12781 atgctataaa gttccttaaa aaaggggata aagtcaaagt tgaacttaga tttagaggaa
    12841 gagaattagg acataaagac ataggtaaag aagttatgct taagttttta gatataataa
    12901 aagagtttgg agaaccaaca aaagcccctg catttgaggg taataatatg gttgtaatta
    12961 tagatccaaa aaaatagatg attaactgag aggaggaatt tgtcatgcct aaaatgaaaa
    13021 ctcatagagg tgcagcaaag agattaaaga aaacaggaac tggaaagtta aagagagcta
    13081 aagcttttaa aaaacatata ttaactaaaa aatctgcaaa aactaaaatg aacttaagaa
    13141 aatcaacttt agtaagtgat ggagatgcta agagaatagc acaattatta ccatactaag
    13201 atacagattg taaataataa ctatagaaga gggatataaa aggaggtcat aacgatggca
    13261 agagttaaaa aagctatgaa cgctcgtaaa aagcataaga aaatattaaa acttgcaaaa
    13321 ggattcagag gatctagaag caaattatat agaccagcaa atacattcgt aatgaaagca
    13381 ttaaaaaatg cttatatagg aagaaagtta aagaagagag acttcagaaa actttggata
    13441 caaagaataa atgcagctgc tagaatgaat ggaatatctt attcaagatt aatgaatgga
    13501 ttaaaattat ctggtgttga agttaacaga aaaatgttat ctgaaatggc tatacaagac
    13561 ccagaaggat ttgcaaaatt agcagaagtt gctaaagcaa aattagctta gtattgaatc
    13621 atgagatgta aagtctttga gttatgtaaa taattcaagg acttttttat tttttgaaaa
    13681 atacttatta gtgtcatgta caaccaattt tataatgata aataaaataa gcaatacaat
    13741 agaattttat gagatttatt ataagaattg ttacaattat ttatgatagc tataaaaagt
    13801 catagtaaaa atgatattga tgtatgttga tatatgtata aagctttcaa tcaatcttga
    13861 attaatatta tcaacatgat aatattatct tattaataat ataaataagg gggagataat
    13921 atgaatgaaa aactaatgga ttgttttgta aacccaatta aatgcaagtt attgattgaa
    13981 attttttcaa ataatcgaac tacagctaaa caattaggag aaaagtttca agatatatca
    14041 aaggcaacac tttatcgtta ccttaataaa atgttgaaag atgatattat aaaagtggtt
    14101 gaagaaaata aaataagggg aactgttgaa aaagtatatg ctatgaagtt agatatagct
    14161 gatgatgtga acaaaattat agatgagaac tctggagaag aatatgctca actgtttact
    14221 caatatatga tgggattttt aagagaattt tatgaatacg ctgaaagaaa ggaaaaggat
    14281 atcctaaaag atggttcagg attttctgtc tgtccagtat atgctagtat tgatgagata
    14341 gttgaagtga gtaaaaaaat tgctagtata ctagaacctc taagaaataa ctcacatgaa
    14401 gaaggtagaa gaatgcattc tataggagtg ataattactc cacctaaaga ggatgaaggg
    14461 aaataaatct gatttacatg gggtagagcc aatttataca gagaatcgtt ttatgattta
    14521 catatgtatc cagagcaaag tatctctgct tggattgtac ttcattgtac catttcatcc
    14581 aatgacttgg agtggtacaa tgaagttttt taatttttca tattaatgct aaagaaatga
    14641 actttagacc acatatgcaa ttaggttgca ttatacaatt agaccacata tacaaaataa
    14701 aaatcagtat ttttatagca gataaattga ctgttattct tcttaaaaat catctattag
    14761 ttattgttag catattgaaa gaaattatag taatctattg aataaatgaa tattgcccat
    14821 aatcatataa actgctatag tttaaaataa agcaggacgt taaattttat agtaatctaa
    14881 aaatttaaga gggggatttt atgaaaataa aatttgttat tatgccattg gtgatatttt
    14941 taatggtatt atttgtgggg tgtcaaagta caaaagatac agaagttaaa gcaaaagata
    15001 cagaaattaa aatattaggg acaggtgatt tacattcaat tttgacagac tctatggttt
    15061 catatataaa tgaggaaagg gaaaagaata aaaatctgct tatggtagat gcgggagatt
    15121 tctatggtac acaaagtaga gaaatgtggg aatggtcttc tggaaaaaag ttaattaata
    15181 ttaaaagaaa tggtagagca gagtatgatg acataattaa gagtagtaaa gatgaagtac
    15241 ctatagttaa agatatggca aagttgaaat atgatgctgt agttttggga gataatgaat
    15301 ttatttcaaa tgataagcaa agtcttgata aactggtttc tgattttaaa aataataata
    15361 tgccattggt atcagcaaat atatatgaac aatctggaga aaattatgta caaccatatg
    15421 taatgaaaaa tattaagaca gatgagggca atgtaaaagt tggaatacta ggtcttacta
    15481 taaaagaagt aggggaaacc cttggtcttg aggattttga aaaggataaa aaagctagag
    15541 agcttggaga acaagctgat tacaaaggaa aattatatgc aaatgattta gtagaagatg
    15601 caaagaaatg gataaaagtt atggaaaaag aaagtccaga cataatagtt gcagttgtac
    15661 actcagggga agaacctaaa acagatagaa atccaggaaa tagaataaaa gagttagcaa
    15721 ctacagtgga tggaatagat gcaattgtag ctggtcatac tcatgaaaaa atagatgaac
    15781 acaaatataa aaataaatct ggagatgaag taattgttac acaaccaggt tggcatggag
    15841 atagagtatc agagattaat ttcaaattaa ataaagagaa tggaaagtgg gacataaagg
    15901 aaaagtctag taaattaaaa ataattgata aagaagtaag tatagttgct actgcagatt
    15961 tacattcaca tttctctgat gaattaacag ttgaccttgt taatgaacgt agtaacccaa
    16021 ttgaacctgt tgtagtagat gcaggcgact tcttagaccc tcaaacagat gaaatgactc
    16081 aatggtataa agagtggaaa agtataagag ataataatag tgataaaacg attagtagat
    16141 gcccaatggt catggatatg aatcagggaa tgtatgatgc agtagtactt ggaaatcatg
    16201 aatttgtatc tgaaaaagat gaattttggt cagatgaacg ccttttagat gctgttatag
    16261 aggattttga acaggtggca atttctgtgt tatcggcaaa tatatatagg caatctggta
    16321 aaaattatgt taaaccatat attataaaag atgtggagac gagtgaaggt aatgtaaaag
    16381 taggaatatt gggacttact ataaaagaag taggtaaagg tcttaaaaat gtaaacttac
    16441 aagagcaatt tcaatataag gataaattgt atgcaaatga tttagtggaa gatgcaaaga
    16501 aatgggtaaa agttatgaaa aagaaaaaac cagatataat agttgcagtt gtacattcag
    16561 gagaagaacc aaaaaatcca aaacatccag gaaatagaat aaaagaacta gcaactacag
    16621 tagatggtat tgatgcaatt gttgctagtc atactcatga aaaaatagat gagcatgaat
    16681 ataaaaataa gtctggagaa aaagttatag tgacacaacc aggtgagcat ggtaaatatt
    16741 attctaaaat tacctttaaa gtaaataaag aaaagggttc gtggactatt aatgataaat
    16801 tcagtaaaac aattgaggtt gaataacttt ataaaattta tagataaagt aaattatctg
    16861 atattgacat gatatataag ataaagtttc taatattggt ataatgcata atgtgaaata
    16921 ttttaataat aagagaataa acaagacaaa taaaaatatt ttaacatgta agatatagta
    16981 tgaactatta tactaatgct tactttataa tttgaacatg ttagaataag tcttatttga
    17041 aaattttagt gtattagagt aagtattatt ttactagtaa aaaatattga gaactatatg
    17101 tagatttgct taaaaaggaa tttacatata gttcttttcg tataataaat agtacaatct
    17161 tatatggtaa agataaagtg tgatattaaa ttttattatc atccaaaatc taaggaggag
    17221 tctcttatga aaataaaatt tattatagta gcatcaataa tatttttaat agtattactt
    17281 gtggggtgtc aaagcactaa agatacagaa atcaatgtac tcgccacaag tgatttacat
    17341 tcaattattc cagataattt aatttcctat gtagaagagg agagaaaata tgatgagaat
    17401 ctacttatgg tagatgcagg agattttttt gacatgcaaa gtggtgagat gaataagtgg
    17461 tttactggtc aaaaattagt aagatttaaa gatgggatac cagagtacaa aaaaatagct
    17521 gaaccacgtg aaggagaagt tccattggct aaaaaaatgg caaagctgaa gtatgatgca
    17581 gttacatttg ggaatcatga atttgtatct aatgacaagc agagccttga taaattggtt
    17641 tctgatttta aaaataacaa tataccatta gtatcagcaa acgtatatga acaatctgga
    17701 gaaaactacg taaagccata tgtaatgaaa aatataaata ctgacaaggg aactgtaaag
    17761 gttggtatac ttggtctgac tataaaagaa gtaggggaac gttcaaggtt taaaagtttt
    17821 gaacaagata aaaaggctag agaacttggg gaacaacctc aatacaaagg gaaattatat
    17881 gcaaatgatt tagtagaaga tgcaaataag tgggtaaatg ttatggaaaa aaaagaaaag
    17941 ccagatataa tagttgccat tgtacattca ggtgaagagc caaaaaatcc taaaaatcca
    18001 ggaaatagaa taaaagaact agcaacaact gtagagggaa tagatgctat tgtagcaggt
    18061 catacacatg aaaaaataga acagcataca tataaaaata attctggtga agaagtaata
    18121 gtaacacagc ctggagctca tggtaatagt atatcaaaga ttaattttaa gttggataag
    18181 aagaatgata agtggtttat aaaagacaaa tatagtgaat taaaggtttt tggaaaggaa
    18241 gtaaatataa ttacaacttc tggtctgaat ccaaaatttt cagatgaaat ggcagttaat
    18301 ttgtttaatg aaagagatag agatgttcaa cctatcttta tagatactgg agatttttta
    18361 aatactaata caaaagaaat gactaagtgg gctaaagagt ggcaatacaa tgaaaataaa
    18421 ggcatctata aaaaaataaa tgaatggcca attgtatatc ttggttatga tgctgtagta
    18481 cttggtagcc atgaatttac agaggataaa gagacttttg aggaaaaaaa atctacattg
    18541 gattatatta tagaaaactt tgaagaaatg aagataccag tattatcagc taatatatat
    18601 gatgaatctg gagaaaatta tgtaaaacct tatataattc ataatgtaga aactagggaa
    18661 ggaaatataa aggttggagt attaggtctt actactactg ataaggatga agaagactta
    18721 agtaactcaa gcttaagcaa agactcaaag acttcgaaaa ttcctgaaga aacaggaaag
    18781 ttatatacta ataatctagt aaaagatgca agcgaatggg taaaagttat gcaaaaggaa
    18841 agtcctgatg tgattgttgc agttgtacat tcagataata atagtactct aaaaaatcca
    18901 agtagtgaag tcaaaaaact tgcaatgtca gtagatggaa tagatgctat tgttgtaggt
    18961 catacgaaaa ataaggtaga agaacatatt tacaaaaata agtctgggga tgaggttata
    19021 gtaacacaat ctggagaaga ttgttattca aaaataagtc ttgaactaag aaaagaaaat
    19081 ggcaagtgga atatcataaa caaatatagt aaagcaataa aaatgaagtg aatttattga
    19141 tatataaacc attattaaca ctgattttta taatataata aatactgatt c
//
LOCUS       DAMJVI010000074        19027 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.076, whole genome shotgun sequence.
ACCESSION   DAMJVI010000074 DAMJVI010000000
VERSION     DAMJVI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19027
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.076"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            100..1701
                     /locus_tag="PC545_002573"
     CDS             100..1701
                     /locus_tag="PC545_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HDF2457337.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADKQTYETVILVNTEKSLADGLSASGLSGATKAPILFTQQNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELDSKNIEVKRIGGEDRLKTSYLIAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKSVPFDVKNIQSYCIGSEEIMSNPLVKNTNSVR
                     IEGTDRFETNKNVIDYFFNSADGFYVSDGYQLVDAIAAAPLTKNSPMVLVNDGSDKTV
                     LEGAKNITSVGEINEKVIQQCINASNSNGQPPTITVGSTEVYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTGKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKEPLLVSSRLEGMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKNVQTTQDAKDLAKAIFEVWKKSPEYNANMLSNEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            1920..3518
                     /locus_tag="PC545_002574"
     CDS             1920..3518
                     /locus_tag="PC545_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2457338.1"
                     /translation="MKVNKRVLSIGLTISLIMAGAPNINALSSIEKIQGKDRYETSAL
                     IADKQIYDTIILVNTDNSIVDGLSASGLSGVAKAPIMLVQRDKIPTDVEKRLKDVKNA
                     YVIGTEDTIGKSVENQLKNKGIEVKRIGGEDRIKTSYLIAKEISAIKPVNDGDKVFLV
                     NGYTGEADAMSVSSVAARDGVPVILTDGKSIPFKVDGVQCYSLGSEEIMSNELVSKTN
                     SVRIAGKDRFETNKKVIQRFYKGTKKFYVSQGYKLVDAVAGSPLAKDKPIVLVNDGSD
                     KSVLRGADEVTSLGGMDKKVVDQCISSASDKNTMPTITANDVEISVGDKFDNSMLNIV
                     ATDYYGNDLKANIKGNVDINKAGTYVLNISVVDNLGQKSEISVNVKVVVNASTKDSNS
                     YEFKAMVSNEMYDLVNSYRKEKGKKSLRELDSLAGMANAWSKYMEDKKVFAHEIDGKN
                     AAEVFFGFGARSGENIAYLPMNVKSVYTSKDAKEMAESIFDLWKKSSKYNENMLKEEF
                     YSFGFGMHVSSKGEVNATMEFLNS"
     gene            3808..6006
                     /gene="slpA"
                     /locus_tag="PC545_002575"
     CDS             3808..6006
                     /gene="slpA"
                     /locus_tag="PC545_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HDF2457339.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPIFADTTVKEEGYTVVQDKYEKLL
                     KELKAKIKDGTITSVGVEFDGKPITTLAPKADGSDKDAIAEQLETLTKNQLKGLGDGK
                     YVDFKITYGAKAEVPAASLSADDIQKYADQINASEKILVEVAAGSEAGIAKFDSVNNK
                     VIAGDAPLKVKDAVKATVTTNGSNKKVLTISAAAGLSGFSYGTLKDTGASSSDVDAIT
                     LDTTNATITEGDTKVLDFDNSFKFNESTKKVGSLVTPNTTNTPADPGTKTTVRVIKAV
                     EKTIDVSSNSTTKAKDLAKQYVFTDVSDTDPESLSYMLKNINDGKVAVKNSDGDYEVT
                     IFPEGKRLNTLSASSAKTILGDKDTPAKIVLKASTTKKLADYIDDLITYNNSYSNVQT
                     VAGSDRIETAIELSRKYYNSTDKNALYGDPVNNVVLVGSQAIVDGLVASPLAAEKDAP
                     LLLSSKDKLDSSTRAEIKRVMDLNSSTGIKNNKEVFIAGGVNSISKDVENELKDMGLK
                     VTRLSGDDRYETSLAIADEIDINDKAYVVGGTGLADAMSIAPVASQIKDGEATPIVVV
                     DGKSDKLSKEAEDFLDDAQVDIIGGENSVSAKMEDYIDDATGKSPERISGADRQATNA
                     EVIKEYFDKDGVSNYFLAKDGSTKEDQLVDALAAAAVAGNYGSKHNEDGDITTDASPA
                     PIILATDNLSAEQHVAVSKTATTNGAKNLVQVGQGIADSVVSKLKDLLDM"
     gene            6252..8594
                     /gene="secA"
                     /locus_tag="PC545_002576"
     CDS             6252..8594
                     /gene="secA"
                     /locus_tag="PC545_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDF2457340.1"
                     /translation="MSVLDVILDKTDELEIKKLNNIVDKIDALEDKIQILDDEALKNM
                     TNVFKDKLSKGETLDDILPEAFAVVREVSKRILGMRQYRVQLIGGIVLHQGKIAEMRT
                     GEGKTLVGVAPVYLNALTGKGVHVITVNDYLAQRDKELMQPVYEFLGLTVGVIINGQQ
                     PVERKLQYQCDITYGTNNEYGFDYLRDNMVTQKSEKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGGGDEGTKLYELANAFIKNIRDEDFEMDRKDKTIALTASGLSKAEKFFGIEN
                     ITHIDNIEIFHHVNQSLRAHKLMNIDVEYVVRDNEILIVDEFTGRIMDGRRFTDGLHQ
                     AIEAKEGVKINDESKTMATVTFQNFFRLYSKLSGMTGTAKTEESEFEATYKINVVQIP
                     TNKPVIRADLHDKVFKTERAKYNAVVEEIIRINKTKQPVLVGTVSIEKSEKLSKLLKK
                     KGVKHEVLNAKQHDKEAEIISKAGKLGSVTIATNMAGRGTDISLGAGDKEEEEKVKEL
                     GGLYVIGTERHETRRIDNQLRGRSGRQGDPGTSRFFVSLEDEVIKLYGGKSIEKILKR
                     MNANEDDALESKALNRAIERAQKGIEGKNFEQRKNVLKYDDTINEQRKVIYDERNKVL
                     DGSDIEEEIQKMVMDIITEAAEKYLKKFRDYHGYFKYLYNTFMPADTLLIPDLDKKNV
                     EEIVNQTYQISKRVYDLKKMMIGIEELAQLEKRVLLQVVDTYWVDHIDAMDQLRQYIG
                     LKSYAQKDPFKEYAIEGYEMFEALNRNIRITTVQYLYKFN"
     gene            9008..10879
                     /locus_tag="PC545_002577"
     CDS             9008..10879
                     /locus_tag="PC545_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2457341.1"
                     /translation="MNRKNLYVIMAAAMVSTSVAPSFAAEATQVKKQTITKKEATELV
                     SKVRELMAQRYTGGSQVGQPIYEIKVGETSSQLKIITNIDELEKLVNALGENKELLVT
                     IADKGHTTNSANEVVAEAIEKYENSADLSAEANSITEKAKTEATGIYGVATVEASYDS
                     TKDKLVITLKDKTDVVTSKTLQIGIGDEKVDLTANPVDIAGTNLDPSAEGFRVSKINK
                     LGVEGAKNIADIQLAEITIKNSDLNTVSPQDLYDGYRLTIRGNTIANSVGKSISDISS
                     KDSETGKYKFTVKYTDTSGKAIELTVESTNDKELKDVKDALNGNTKVNLIAGEDRYAT
                     AVAIAKETEYTDNIVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKEI
                     IKKSPSAKIYIVGGESAVSNTARKQLESVTKNVERLAGDDRHTTSVAVAKAMGSFKEA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLTADAIKLMDGKEIGIVGGSNNVPSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKNDITNSQKNTLDKKLNLGAEVTQIGNGVELTVVQKIGKILGW"
     gene            10988..11695
                     /locus_tag="PC545_002578"
     CDS             10988..11695
                     /locus_tag="PC545_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2457342.1"
                     /translation="MIEATLFFSPHQDDETLSMGSAIIEHVERSDTHVILCTDGSKSI
                     IRKVLDDGGSCSYHIKDVHKYSLSESDFSKNRDEEFKNSCKAMGVKENNIHIENNRAH
                     DGELSKEKARDIMLKYLEKYPDAKVKTVTPYKALGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYNDFKKVNPNVRVWKVLSSQEEKLLNAMDAYKKWNPENGNYAIGYHSVK
                     SHFDELKSNKIQYVHAP"
     gene            11728..13860
                     /locus_tag="PC545_002579"
     CDS             11728..13860
                     /locus_tag="PC545_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2457343.1"
                     /translation="MKISKRIMSLLTATFFIFALSINIADASSKESLVGSGRWETAIK
                     ISQNGWKDATNAILVNDNSIADALSATPFAKLKDAPILLTQSDKLDSRTKAELKRLGV
                     KNVYLIGGMNTLSTNIEKQLKSENMTFERISGNSRYETSLKLAERLDKEKSISDIVVV
                     NGEKGLADAVSVGSAAAQENMPIILSDPNNGIKVANSFISSKNIKKSYIIGGTYSVSS
                     SIEQSLPNVKRLSGNNRNETNAKVLEEFYKNTDLKNAYITKDGMKKQGDLIDSLAVGV
                     LAAKNSSPVVLVGNSLDSSQYDVLNTKSFDNIVQVGGNGNEDAVKDILDLQEETIYRV
                     KTADELDVALRKADANDIITFKPDDNKKITSSFSIETKKAITVELEGLYTQTITVNMP
                     NGELTNKGTLDGTVKIMDIKNGTFINEGDINDIDIYDPNGCKIENKSGGDIWVLTISS
                     SAKDVNIENKGDISKVTNNSTSVIIRNSGNIDTLSGINAPAISGNKPRVDDTDKDEKR
                     ASGIYPTSEPLSPARAGYISVKIFTKPEDTNYKIYYRVVSSRPSALYIGDRIDTSDWT
                     AVPSSVLPFTIKAENGYYIEAVEINSDTKKVSRWGKSDYIDDGFYLKSETAKGLSADI
                     DFDGDYVKLTATGADSGCTVYYRIVSKKPVSMNVGDKVTISDWDEITTNRVRIEANDV
                     KDKYIELVELDDSTNKVTRWGASDKVVE"
     gene            complement(13913..14308)
                     /locus_tag="PC545_002580"
     CDS             complement(13913..14308)
                     /locus_tag="PC545_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDF2457344.1"
                     /translation="MILKKHRETILYLFFGAFTTLVNIVSYLFFTRVILFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELIFLFKEFLFFVSFRLLSGIIEMIIMYIMIDLFF
                     VNDITVKVFTNIIVIVLNYLFSKMIIFRK"
     gene            14535..16946
                     /locus_tag="PC545_002581"
     CDS             14535..16946
                     /locus_tag="PC545_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HDF2457345.1"
                     /translation="MRKYKSKNLSKLLALLTVCFLIVSTIPVSAENHKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRNKGRSSLPSAYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATKPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHAVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKAELQRLGTKKVYLIGGENSLSKNVQTQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKSQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKNVLKSKKIETPIRVGGNGNENAFNELN
                     TLLGK"
     gene            17243..18820
                     /locus_tag="PC545_002582"
     CDS             17243..18820
                     /locus_tag="PC545_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HDF2457346.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLIGNGRYETAVK
                     ISQKAYSSSPNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTEKEIKRLGA
                     KDIYLIGGTAVLNKDIENKLKSNGLNVERINGKDRYETSLILANKLKDIKDIKEVAVV
                     NGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVIGGTNSVSR
                     AVEKSLPNAERVSGKDRNETNAKVIEKFYTDTNLSNLYVTKDGSKNENQLIDSLAVGV
                     LAAKNESPVVLVGNKLNTKQRDILSTKKLNTITQVGGNGNEEAFDEIKSLQEKTVFEA
                     KTVEELTDMINIASPNDIINFKPKENTVNEAFRMVTNKPITVNIKGDCSKTLTVDMPN
                     GEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDIDTLMILASA
                     NDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAIDSISQKVN
                     KIQDILDKLGFLKKFLS"
BASE COUNT         7743 a         2126 c         3585 g         5573 t
ORIGIN      
        1 ttcatatcat atatactcta taatatatgg aaaataaaga gaaaataaat ggaaaataat
       61 cacaatataa atataaaata taaaaatcat cagggggata tgaaaatgaa caaaaaaata
      121 ttatcattag gtctagcagt atcattaatc ttagtaaact tcaaaagtgt aaatgcatca
      181 tcagtagtag aaaaaatata cggtaaagat agatatgaaa cagcagcaaa gatagctgat
      241 aaacagactt atgaaacagt aattttagta aatacagaaa aatcacttgc agatggatta
      301 agtgcaagtg gattatcggg agctacaaaa gctccaatac tatttacaca acaaaataag
      361 ataccagcag acacaaatag atgtctaaaa aatatcaaaa aagcatatat aataggtaca
      421 gaagatacta taagtaaatc agtagaaaaa gaactagatt ctaaaaatat agaagtcaaa
      481 agaattggtg gagaagatag actaaaaaca agctatttaa tagctaaaga aatagcaact
      541 ataaaaaaag tggataaagt actattaact aatgcatata gtggagaagc agatgcaatg
      601 agtgtatcat cagtagctac tagagatgga gctccaatca tacttacaga tggaaagagt
      661 gtgccttttg atgtaaaaaa tattcaatca tattgtatag gttcagagga gataatgagt
      721 aatcctctag ttaaaaatac aaactcagta agaatagaag gaactgaccg atttgaaact
      781 aacaaaaacg taatagacta cttttttaat agtgcagatg gattctatgt atcagatgga
      841 taccaattag tagatgcaat agcagctgca ccacttacta aaaactctcc aatggtacta
      901 gtaaatgatg gaagcgataa aactgtatta gaaggagcta agaatataac ctctgtaggt
      961 gaaataaatg agaaggtaat acaacagtgt ataaatgctt caaattcaaa tgggcaaccc
     1021 ccaacaatta cagttggaag tacagaggta tataagggtg aaaagtttga cactggcaag
     1081 ttaaatatag tagctaaaga taacacaggg aaggtattac caatagaagt tgatgggttt
     1141 atagatacaa atagagtagg tacatacata ttgacattaa aagctacaga cgaatgggga
     1201 aaaagcacag gaaaaagagt agaaataaaa gtgttggatg acaaatctca tgattacaac
     1261 agcccagaat ttaaaaaaat ggtatctact gaaatgtata acttaatcaa ttcctataga
     1321 aaagaaaaag gcaaagagcc tttgctagtg tctagtaggc tagaaggtat ggcaaatgca
     1381 tggtctaagt atatgatgga taaaaaagta tttgcacatt atatagatgg aaaaaatgct
     1441 ccacaagtat tttctgaatt tggaatgaga agtgaggaaa atatagcata tatttatata
     1501 gattctaaga acgttcaaac gacacaagat gcaaaagact tggcaaaggc tatatttgag
     1561 gtgtggaaga agtctccaga gtacaatgca aacatgctta gtaatgagtt ttatagtact
     1621 ggatttgggc tttatatatt atctgatggt caagtgcatg ctactcagga gtttttaaat
     1681 ggaaatgaag gtagcttgta aaaaattcca taaatcttgt ttttttgtta atttactgat
     1741 attataaaat gaaaatcaca tctaaagtac tttttgtaat ctgttttgtt attgctttac
     1801 aggctatggt attttaggtg ttttttgttt tagtagttta tagattttga gggaatcact
     1861 actgaggatg taatttatga ttagctgaat tatataaaaa aattagtagg aggagtttga
     1921 tgaaggtaaa taagagagta ttatcaatag gattgacaat atcattaata atggcaggag
     1981 caccaaatat caatgcactt tcgagcatag aaaagataca ggggaaagat agatatgaga
     2041 cttctgcatt gatagcagat aaacaaatat atgatacaat catattagta aatactgata
     2101 attctatagt agatggatta agtgcaagtg gattatctgg agttgcaaaa gctcctataa
     2161 tgttggtgca aagagataag ataccaacag atgttgaaaa aagactaaag gatgttaaga
     2221 atgcttatgt tataggtaca gaggatacaa taggtaaatc tgtagaaaat caacttaaga
     2281 ataaagggat tgaagttaaa agaattggtg gggaagatag aataaagact agctatctta
     2341 tagctaagga gatttcagct attaaaccag taaatgatgg tgataaggtt tttcttgtaa
     2401 atggatacac aggtgaggct gatgcaatga gtgtatcttc tgtagctgct agagatggag
     2461 ttcctgtgat acttactgat ggaaagagta taccatttaa agttgatggc gttcagtgct
     2521 attctcttgg gtcagaggag ataatgagta atgaacttgt gagtaagact aattcagtta
     2581 ggattgctgg taaggatagg tttgaaacta ataagaaggt tatacaacgc ttttataaag
     2641 gaacaaagaa gttttatgtt tctcaagggt ataagttagt tgatgctgtg gctggttctc
     2701 cgttggctaa ggacaaacct atagtacttg ttaatgatgg aagtgataag agtgtgctta
     2761 gaggtgctga tgaagttact tctttaggtg gaatggataa gaaggttgtt gaccaatgta
     2821 ttagttctgc atctgataaa aatacaatgc ctacaattac tgctaatgat gtagagattt
     2881 ctgtagggga taagtttgat aatagtatgt tgaatatagt tgctactgat tattatggaa
     2941 atgatttaaa ggcaaatatt aaaggaaatg ttgatattaa caaggctgga acatatgttt
     3001 taaatataag tgtagtggat aatttaggtc agaagagtga gattagtgta aatgttaagg
     3061 tagttgtaaa tgcgagtaca aaagactcta atagttatga gtttaaggct atggtatcta
     3121 atgagatgta tgatttagtt aattcatata gaaaagaaaa aggcaagaag agtttaagag
     3181 agttagactc tttagctgga atggcaaatg catggtctaa atatatggaa gataagaagg
     3241 tatttgccca tgagattgat ggtaaaaatg cagctgaagt attttttggc tttggagcaa
     3301 gaagtggtga aaatattgca tatctaccaa tgaatgttaa atctgtttat acttctaaag
     3361 atgcaaaaga aatggctgag tctatttttg atttatggaa gaagtcttct aagtataatg
     3421 aaaatatgtt aaaagaagaa ttttatagct ttggatttgg tatgcatgtt tctagtaaag
     3481 gagaagtgaa tgcgacaatg gaatttttaa atagttagat tttatattaa tttacattgg
     3541 aaaagatata gtaaatttgt aaaaataaat atataaaatt aaaataactt ataaaattat
     3601 aaaaaataat ttaaaaataa taaaaatgat gaaaaaacaa ttaatttgtt ggaaaaaact
     3661 tcaattaatt ttgcataaat tattgaattt atattttata tagtctatca taagagtgtg
     3721 agaaatattt ggattatatg aacatggaaa tttaatgtac ataattaaag agatgtaatt
     3781 aatataatgt tgggaggaat ttaagaaatg aataagaaaa atttagcaat ggcaatggca
     3841 gctgttactg ttgtaggttc tgctgctccg atatttgctg atactactgt taaagaagaa
     3901 ggatatacgg tagtacaaga caaatatgaa aaattactta aagaattaaa ggcaaagata
     3961 aaagatggaa ctattacatc agttggtgtt gaatttgatg gaaaaccaat tactacatta
     4021 gcaccaaagg cagatggtag tgataaagat gcaatagcag aacagttaga aactcttact
     4081 aaaaaccaac ttaaaggttt aggtgatgga aaatatgtag attttaaaat tacttatggt
     4141 gctaaagcag aagttccagc tgcttcatta agtgcagatg atatacaaaa atatgcagat
     4201 caaataaatg cttctgagaa aatattagtt gaagtagcag caggatctga agcaggtata
     4261 gcaaaatttg attctgttaa caataaagtt atagcaggag atgctcctct taaagttaaa
     4321 gatgcagtga aagcgactgt tactactaat ggtagtaata aaaaagttct tacaatatca
     4381 gctgcagctg gattatcagg attttcatat ggaacactta aagacacagg tgcaagttca
     4441 tctgatgttg atgcaataac tcttgacact acaaatgcaa caataacaga aggtgatact
     4501 aaagtattag attttgataa ttcatttaag tttaacgaaa gtactaaaaa agttggtagt
     4561 ttagttacac caaatacaac aaacactcca gcagatcctg gtactaaaac tactgttaga
     4621 gtgataaaag ctgttgaaaa aacaatagat gtaagttcaa attcaactac aaaagctaaa
     4681 gatttagcta aacaatatgt attcacagat gttagtgata ctgatccaga aagtttaagt
     4741 tatatgctta aaaacataaa tgatggaaaa gttgctgtta aaaattctga tggagattat
     4801 gaagtaacta tattcccaga aggaaaaaga ttaaatactt tatctgcttc ttcagctaaa
     4861 actattttag gagataaaga tactccagca aaaatagttt taaaggcttc aacaacaaag
     4921 aaattagcag actatataga tgatttaata acttataaca atagttattc aaatgtacaa
     4981 actgtagcag gtagtgatag aatagagact gctatagagc taagccgtaa gtactataac
     5041 tctactgaca aaaatgcttt atatggagat cctgtaaata acgttgtatt agtaggttct
     5101 caagctatag ttgatggatt agtagcatct cctttagcag cagaaaaaga tgctccttta
     5161 ttgttaagtt ctaaagataa attagattca tcaacaagag ctgagataaa aagagttatg
     5221 gacttaaatt cttcaacagg aataaaaaat aataaagaag ttttcatagc aggtggagta
     5281 aattctatat ctaaagacgt tgaaaatgaa ctaaaagata tgggacttaa agtaactaga
     5341 ttatcaggtg atgatagata tgaaacttca ttagcgatag ctgatgaaat agatataaat
     5401 gataaagctt atgtggtagg tggaacagga ttagcagatg ctatgagtat tgctccagtt
     5461 gcatctcaaa taaaagatgg tgaagctact ccaatagtag ttgtagatgg aaaatctgac
     5521 aagttatcta aagaagctga agatttctta gatgatgcac aagtagatat aatcggtgga
     5581 gaaaatagtg tttctgctaa aatggaagat tatatagatg atgctactgg aaaatctcca
     5641 gaaagaatta gtggtgctga tagacaagca actaatgctg aagttataaa agaatacttt
     5701 gataaagatg gagtttcaaa ttacttctta gcaaaagatg gttcaactaa agaggatcag
     5761 ttagttgatg cattagcagc agcagcagta gctggaaact acggttctaa gcataatgaa
     5821 gatggagata taactacaga tgcttctcct gctccaatta ttttagcaac tgataattta
     5881 tcagctgaac aacatgtagc tgtaagtaaa actgctacta ctaatggtgc taagaactta
     5941 gtacaagtag gtcaaggaat agctgattca gttgttagca aattaaaaga tttattagat
     6001 atgtaatata aattttatat attgaataaa aaaggtttct cttttgagaa acctttttta
     6061 ttttaaacat aaatttttat aaataaaaag aggctaatta gcctcttttt aatgtgagaa
     6121 acacttaaaa aataagatgt aattatttat ttaagtatca ttattatact ataccttaaa
     6181 ataaatgtct actattctat aattttaagg tataatagta agtggaaaat aaataaggag
     6241 gtaaattaaa tatgtcagtt ttagatgtaa tattagacaa aacagatgaa ttagaaatta
     6301 aaaaattaaa taatatagta gataaaatag atgctttaga agataaaata caaatactag
     6361 atgatgaagc attaaaaaat atgacgaatg tatttaagga taagttaagt aaaggagaaa
     6421 ccttagatga catattgcca gaagcatttg ctgttgtaag agaagtatca aaaagaatat
     6481 taggtatgcg tcaatataga gttcaattaa ttggtgggat tgtattacat caagggaaaa
     6541 tagcagagat gagaactgga gaaggaaaga ctctagtagg agtagctcca gtttacttga
     6601 atgcattgac tggtaagggg gtacatgtca tcacggttaa tgattacttg gctcaacgtg
     6661 ataaagaact tatgcaacca gtatatgaat ttttaggttt aacagtagga gtaattatta
     6721 atgggcaaca gccagtagaa cgaaaattac aatatcaatg tgatattact tatggtacaa
     6781 acaatgagta tggattcgat tatttaagag ataatatggt aacgcaaaaa tcagagaaag
     6841 ttcaaagaga actaaacttt gctatagttg atgaggttga ttcaatactt atagatgaag
     6901 ctagaacacc acttataatt gctggtggag gagatgaagg tactaagctg tatgaacttg
     6961 caaatgcttt tataaagaat ataagagatg aagattttga aatggataga aaagataaaa
     7021 ccatagcact tactgcaagt ggtcttagta aagctgagaa attttttgga atagaaaata
     7081 taactcatat agataatata gaaatttttc accatgtaaa tcaatctcta agagcacata
     7141 aattaatgaa catagatgtt gaatatgttg ttagagataa tgagatacta attgtagatg
     7201 aatttacagg aagaataatg gatggaagaa ggtttactga tgggcttcat caagcaattg
     7261 aagctaaaga gggagtaaaa ataaatgatg aatcaaagac catggcaaca gtaacatttc
     7321 aaaatttctt tagattatac tctaaattat caggaatgac aggtacagca aagacagagg
     7381 aaagtgagtt tgaagctaca tataagatta atgttgttca gataccaact aataaaccag
     7441 ttataagagc tgatttacat gataaagtat tcaaaacaga acgtgcaaag tacaatgctg
     7501 ttgtagaaga aataataagg ataaataaaa ctaagcaacc agtgcttgta ggaactgttt
     7561 caattgaaaa atctgagaaa ttatctaagc tacttaaaaa gaaaggtgta aagcacgagg
     7621 tcttaaatgc taaacaacat gataaggaag ctgaaataat atctaaagct ggtaaattag
     7681 gttctgttac aattgctact aatatggcag gtagaggaac tgatatatca cttggagctg
     7741 gagataaaga agaagaagaa aaggtgaaag agttaggtgg cttgtatgtt ataggtactg
     7801 aaagacatga gacaagaaga attgataatc agcttagagg gcgttcggga agacaaggtg
     7861 accctggaac ttctagattc tttgtaagtt tagaggatga agtaataaaa ttgtatggtg
     7921 gaaaaagtat agaaaaaata ttaaagagaa tgaatgctaa tgaagatgac gcgttagaaa
     7981 gtaaggcttt gaatagagca atagaaagag cacagaaagg cattgaaggt aaaaattttg
     8041 aacaaagaaa gaatgttctt aagtacgatg atactattaa tgagcaaaga aaagtaatat
     8101 atgatgaaag aaataaggta ttggatggtt ctgatattga agaagagata caaaaaatgg
     8161 ttatggatat tattacggag gctgctgaaa agtacttaaa aaaatttaga gactatcatg
     8221 gatattttaa gtatttatat aatactttta tgccagctga tactttatta attccagact
     8281 tagataaaaa aaatgttgaa gaaatagtaa atcaaacata tcagatttca aagcgtgttt
     8341 atgatcttaa gaagatgatg attggaattg aagaattggc tcaattagag aaacgagtat
     8401 tacttcaagt tgtggatact tattgggttg accatataga tgcaatggat caactaagac
     8461 aatatatagg tcttaaatct tatgcacaaa aagatccatt taaagagtat gctatagaag
     8521 gttatgaaat gtttgaagct ttaaatagga atataagaat aacaacagtg caatatctat
     8581 ataaatttaa ctaaaaaagt gacccattgg gtcacttttt atatattaag attaaagtga
     8641 aatttatttt acagtacaca ctcatataga taaatattta taataatgaa aaagaggctg
     8701 aacgtcagcc tcttttcctg tgtgagaaat acggaaaaaa gagatgcttg aaatttcttt
     8761 cttccattta atattatact ataaagagaa taaaaagtcc atgagttatc tttatgatta
     8821 aaaaaaaaaa ttacaatttt gttacttttt agtatacaat aataaattta aattgacttt
     8881 gttaattttt attaataaaa tatccaaata ttacaatatc tacagtttat tgaaataatg
     8941 tgtaaattat attataattg gtaaataagg aaaaatggtg aaaaccaaat tttgtagggg
     9001 gaaatatatg aatagaaaaa atctttatgt aattatggca gctgcaatgg taagtacatc
     9061 agtagctcca agttttgctg cagaagctac acaagtaaag aaacaaacaa taactaaaaa
     9121 ggaagcaaca gaattagttt ctaaagttag agaactaatg gctcagagat atacaggagg
     9181 ttctcaagtt ggacaaccaa tatatgaaat aaaagttggt gaaacttcat cacaattaaa
     9241 aataataact aatatagacg aattagagaa attagtaaat gctttaggag aaaataaaga
     9301 gcttcttgtg actatagcag ataaaggaca tacaacaaat agtgcaaatg aagtggttgc
     9361 tgaagcaatt gaaaaatatg aaaattcagc agacctttca gctgaagcta attcaataac
     9421 agaaaaagct aaaactgaag ctactgggat ttatggagtg gcaactgtag aagcttcata
     9481 tgacagtaca aaagataagt tagtaataac tttaaaagat aagactgatg tagtaacttc
     9541 taaaacgcta caaataggta ttggtgatga aaaggttgat ttaacagcaa atccagttga
     9601 tatagcagga accaatttag acccttctgc agaaggattt agagtaagta aaataaataa
     9661 attaggtgta gaaggagcta aaaatattgc tgatattcaa ttagctgaaa taactataaa
     9721 aaatagtgat ttaaatacag tttcacctca agatttatat gatggatata gattgactat
     9781 tcgaggtaat acaatagcaa atagtgtagg aaaatcaatt agtgatattt catcaaaaga
     9841 ttcagagaca ggaaagtata aatttactgt taaatatact gatacatctg gaaaagcgat
     9901 agaacttact gtagaaagta ctaatgataa agagttaaaa gatgttaaag atgccttaaa
     9961 tggaaataca aaagtcaatt taatagctgg ggaagacagg tatgcaactg ctgtagctat
    10021 agcaaaagaa acagaatata ctgacaatat agttatagtt aattcaaata aattagttga
    10081 tggattagca gcaacaccac tagctcaatc taaaaaagct cctatattgt tggcgtctga
    10141 taatgaaata ccaaaagtaa ctttagatta tataaaggaa ataattaaaa aaagtccatc
    10201 agctaaaata tatatagtag gtggagaatc agcagtatca aatacagcta gaaagcaatt
    10261 agaatcagta actaagaatg ttgaaagatt ggctggagat gatagacata caacttcagt
    10321 agcagtagca aaagctatgg gttcttttaa agaagcattt gtagtaggtg caaaagggga
    10381 agctgatgct atgagtatag ctgctaaagc tgctgaactt aaggctccta taatagtaaa
    10441 tggttggaat gatcttacag cagatgctat caagttgatg gatggaaaag agattggtat
    10501 agttggtggt tctaataatg tacctagcca aattgaaaat caacttgctg atattgataa
    10561 agatagaaag gttcaaagag ttgaaggtga aacaagacac gatactaatg ctaaagttat
    10621 agaaacatat tatggcaaat tagataaact atatatagca aaagatggat atggaaataa
    10681 tggtatgtta gtagatgcat tggcagcagg tcctctagca gcaggaaaag gaccaattct
    10741 tttagctaaa aatgatataa caaactcaca aaagaatact ttagataaaa aactaaatct
    10801 tggtgcagaa gtaactcaaa taggtaatgg ggttgaattg actgtagttc aaaaaatagg
    10861 caaaatacta ggatggtaat aatcaataat atagaaaagg attccttatt atttatagga
    10921 atctttttct atcgacataa tattcattaa agtataacat gctcttgaca gataggagga
    10981 tatgatagtg atagaagcaa cattgttttt ttccccacac caagatgatg aaacattaag
    11041 tatgggtagt gcaataattg aacatgtaga aagaagcgat acacatgtta tattatgtac
    11101 tgatggcagt aaatcaataa taagaaaagt attagatgat ggagggtcat gttcttatca
    11161 tattaaagat gtacacaagt actctttatc agaaagtgat ttttcaaaaa atagagatga
    11221 agaatttaaa aatagttgta aagcaatggg agttaaagaa aataatatac atatagaaaa
    11281 taatagagct catgatggag aattgagtaa ggaaaaagca agagatataa tgctaaaata
    11341 tttagaaaaa tatcctgatg ctaaagtgaa gaccgttaca ccatataaag ctcttggaat
    11401 acatgaagac catagagcat taggtgaagc tgcattagaa ttgtatagag aaggaaaaat
    11461 aaaagattta agattttatg tagagccata cgattataat gattttaaaa aagttaatcc
    11521 aaatgttaga gtatggaagg ttttgagtag tcaagaagag aaattattaa atgctatgga
    11581 tgcatacaag aaatggaatc ctgaaaatgg taactacgca attggatatc attctgtaaa
    11641 atctcacttt gatgaattaa aatccaataa aatccaatat gtacatgctc cataaacatt
    11701 aatataaaaa ctaggaggat atagttaatg aaaatatcta agagaataat gtcgttgttg
    11761 acagcaactt tttttatctt tgcattatct atcaatatag ctgatgcttc atcaaaagag
    11821 agtctagtgg gctctggtag atgggaaact gcaataaaaa ttagtcagaa tggatggaaa
    11881 gatgctacta atgctatact agtaaatgat aattctatag ctgatgcatt atctgcaact
    11941 ccatttgcta agttaaaaga tgcacctatt ttattaactc aaagtgataa attggatagt
    12001 agaactaaag cagaattaaa aagactaggt gtaaaaaatg tatatttaat tggaggtatg
    12061 aatactctaa gcaccaatat tgaaaaacaa ttaaaatcag aaaatatgac ttttgagagg
    12121 atttcaggta atagtagata tgaaacatct ttaaaattag ctgaaagatt agataaagag
    12181 aagagtattt ctgatatagt tgtagttaat ggagagaaag ggctagcaga tgcagtaagt
    12241 gtgggttctg ctgcagcaca agaaaatatg ccaattatac tttcagatcc taacaatgga
    12301 ataaaagttg caaatagctt tattagtagt aaaaatataa agaaatctta tataattggt
    12361 ggtacatatt ctgtatctag ttctattgaa cagagtttac ctaatgtaaa aagattatct
    12421 ggaaacaata gaaatgaaac taatgcaaag gttctagaag aattttataa aaatacagac
    12481 ttaaaaaatg cttatataac taaagatggt atgaaaaaac agggggattt gatagattct
    12541 ctagctgtag gtgtattggc agctaaaaat agctctccag ttgtattagt aggaaattca
    12601 cttgactcta gccaatatga tgtgttaaat actaaaagtt ttgataatat tgtgcaagtt
    12661 ggtggaaatg gaaatgaaga tgctgtaaag gatattcttg atttacaaga agaaactata
    12721 tatagagtta agacagcaga cgaattggat gttgctctaa gaaaagcaga tgctaatgat
    12781 attataacat ttaaacctga tgataataaa aaaattacta gttcatttag tatagaaaca
    12841 aagaaagcta taactgtaga gcttgaagga ttgtatacac aaacaataac tgtaaatatg
    12901 ccaaatggag aattaactaa taagggtaca ttagatggaa ctgtgaaaat aatggatata
    12961 aaaaatggca catttatcaa tgaaggtgat ataaatgata ttgacattta tgatccaaat
    13021 ggatgtaaaa tagagaataa aagtggtgga gatatatggg ttttaactat atctagtagt
    13081 gctaaagatg taaatataga aaataaaggt gatataagta aggttacaaa taattctact
    13141 agcgttataa taagaaattc aggaaatatt gatactttaa gtggaattaa tgcaccagca
    13201 ataagtggaa ataaacctag agttgatgat accgataagg atgaaaaaag agcaagtgga
    13261 atatatccta cttcagaacc tttatcacct gcaagagctg gttatataag tgtaaaaata
    13321 ttcactaagc ctgaagatac aaattataaa atttattata gagtagtaag cagtagacca
    13381 tctgcattat atatcggaga tagaatagat acaagtgact ggactgctgt accatcttct
    13441 gttttaccat ttactattaa agcagaaaat ggatattata tagaagctgt tgaaataaat
    13501 tctgatacta agaaagtatc tagatggggg aaatctgact atatagatga tggattttat
    13561 ttaaaatcag aaactgcaaa aggattaagt gcagatatag attttgatgg agattatgtt
    13621 aaattaacag caacaggtgc tgattctgga tgtacagttt attatagaat agtatcaaaa
    13681 aagccagtat caatgaatgt tggagataaa gtaactatat ctgattggga tgaaattact
    13741 actaatagag tgaggataga agcaaacgat gtaaaggata aatatataga actagttgaa
    13801 ttggatgatt caactaataa agtaactaga tggggagcaa gtgataaggt agtagaatag
    13861 gtatctaatg tgaaacataa aaacagactg attaataatg ggcagtctgt ttttatttcc
    13921 taaaaattat cattttacta aataaataat ttaaaactat aacaattata ttagtaaata
    13981 cttttacagt aatatcattt acaaaaaata aatcaatcat tatatacata ataatcattt
    14041 ctataatacc agaaagtagc ctaaaactga caaaaaacaa aaactcctta aacaaaaata
    14101 ttagttcaat tcttttactc tcaaacacaa aaaacttatt agtaacataa gcaaataaaa
    14161 cagcaagtat ccatgccaaa gcatttgcaa ccataaaatt aaataaaata actctagtaa
    14221 aaaataaata cgaaactata tttacaagag tagtaaatgc tccaaaaaat aaatataata
    14281 tcgtctctct atgctttttt aaaatcaaat ataatcctcc taagctaatt taatataaat
    14341 atacttaaag atactaacaa acattagtat aacacatcta ttaataaatc aatatatctt
    14401 aaaatattat aaaaataaaa ctataatatt ctataattaa tatgtactca taatccatat
    14461 tttatcaaaa taaaatatga ccttaattta gaaatctgat ataataaaaa ttaaatacat
    14521 ataagggggt aaacatgaga aagtacaaat caaaaaactt gtcaaagcta ctagcattgt
    14581 tgacagtttg ctttttaata gtgtcaacaa tacctgtctc agcagaaaac cataaaactc
    14641 tagatggagt agaaactgca gagtattctg aaagctatct tcaataccta gaagatgtca
    14701 aaaatggaga cacagcaaaa tataatggag taataccttt tccacatgaa atggaaggta
    14761 caacacttcg aaataagggt agaagtagtc ttccatcagc atataaatca agtgtagcat
    14821 acaacccaat gaatcttggt cttactacac cagcaaaaaa tcaaggaagt cttaatacat
    14881 gctggtcttt ttcaggtatg tcaacgttag aagcatatct taaattaaaa ggatatggaa
    14941 cgtatgactt atcagaagaa catttaagat ggtgggcaac tggtggaaaa tatggatgga
    15001 acttagatga tatgtcaggg agttcaaatg taacagctat aggatatctg acagcatggg
    15061 caggtcctaa gttagaaaag gacataccat ataatcttaa atctgaggca caaggtgcaa
    15121 ctaaaccttc aaatatggat actgcaccta ctcaatttaa tgtaacagat gttgttcgtc
    15181 ttaataaaga taaggaaact gtaaagaatg ctataatgca atatggttca gtgacatcag
    15241 gttatgcaca ttattcaaca tattttaata aagatgaaac agcatataac tgtacaaata
    15301 agagagctcc attaaatcac gctgtagcga tagtaggatg ggatgataat tattcaaaag
    15361 ataattttgc atctgatgta aaaccagaat caaatggagc atggttagta aaaagtagtt
    15421 ggggagagtt taattctatg aaaggattct tctggatttc ttatgaagat aaaactctac
    15481 taacagatac agataactat gcaatgaaat cagtatcaaa accagatagt gataaaaaaa
    15541 tgtaccaact tgaatatgct ggtcttagca agataatgtc aaataaagta acagcagcaa
    15601 atgtatttga ttttagcaga gactctgaaa aacttgattc tgttatgttt gaaacagact
    15661 ctgtaggagc aaaatatgaa gtatattatg caccagtggt aaatggagtt cctcaaaaca
    15721 attcaatgac aaaacttgca agtggaacag tatcatattc tggatacata aatgtaccta
    15781 ctaattctta cagcttacca aaaggtaaag gagcaatagt agtagttata gacaacacag
    15841 caaatcctaa tagagaaaaa tcaactttag catatgaaac taatatagat gcatattatt
    15901 tatatgaggc taaagcaaac ttaggtgaaa gctacatact tcaaaacaat aagtttgagg
    15961 atataaatac atatagtgaa ttttctcctt gtaactttgt tataaaagct ataacaaaaa
    16021 catcttctgg acaagctact tcaggagaat ctttaactgg agcagataga tatgaaacag
    16081 cagttaaagt tagtcaaaaa ggatggactt cttcacaaaa tgcagtatta gtaaatggag
    16141 atgcaatagt ggatgcttta acagctacac catttacagc agcaattgac tctccaattc
    16201 ttttgacagg aaaagataac ttagattcaa aaactaaggc agagttacaa agattaggaa
    16261 ctaaaaaagt ttatctaata ggtggagaaa attctttaag caagaatgta caaactcaac
    16321 ttagtaatat gggtatatca gtagaaagaa tttcaggtag tgatagatac aagaccagta
    16381 tatctctagc tcaaaagtta aatagtataa aatctgtttc acaagttgca gtggcaaatg
    16441 gtgtaaatgg acttgcagat gcaataagtg ttggtgcagc agctgctgat aacaatatgc
    16501 caataatact tactaatgaa aaaagtgagt tgcaaggtgc tgatgagttt ttaaattcat
    16561 ctaaaataac taagtcttat ataattggtg gaacagctac tttatcatca aatttagaaa
    16621 gtaagctttc aaatccaaca agacttgcag gcagtaatag aaatgaaact aatgctaaga
    16681 taatagataa attctaccct agctcagatt tgaaatatgc ttttgttgtt aaagatggtt
    16741 caaagagtca aggagacttg atagatggac tagcagtagg agcattgggt gctaaaactg
    16801 attcaccagt agttctagtt ggaaataagt tagatgaaag tcaaaaaaat gtacttaagt
    16861 ctaagaaaat agaaactcct attagagttg gtggaaatgg aaatgaaaat gcttttaatg
    16921 aactaaatac tcttttagga aaatagtgaa tggaaagata gtaaggatat acattttatt
    16981 attaaaaaat gttcaataga agtttataaa tataaaataa caaaaagggg tgtctcaaaa
    17041 tgaacttttt agttcatgag gcactctttt ttgttgtttt atatttttat catttaaata
    17101 atgaaaaata ggattgtcta tatttttaga tagttttctt attattgatg aaaaatgtaa
    17161 taattttaaa ctgtaaaata gtgtaaagtt attttataat ataaatatag taagatttta
    17221 aacaaaataa gggggaataa taatgaaagc accaaaaact attttaacaa tactgacaat
    17281 agcgcttact ttaagtagca tttctataat accatcatat gcacttacag aggaaaaatt
    17341 aataggtaat ggtagatatg aaactgctgt aaagataagt caaaaggcat acagttctag
    17401 tcctaatgta gtgctagtta atgacaattc attagctgat gcactttcag ctacaccatt
    17461 tgcaaaggct aaaggagcac caatactttt gacagaaagt gataagctag atgataggac
    17521 tgaaaaagag ataaaacgtc taggagctaa agatatttat ttgataggag gtactgctgt
    17581 acttaataaa gatatagaga acaagttaaa aagtaatgga cttaatgtag aaagaattaa
    17641 tggtaaggat agatatgaaa cttcactgat tttggctaat aagcttaagg atataaaaga
    17701 tattaaggaa gttgctgtag taaatggtga aaaaggattg agtgatgctg ttagcgttgg
    17761 ggcaccagca gctcaaaata agatgcctat aattttgtct agtcctaagg atggagtaga
    17821 agcatttgat aaatttataa gggatgagaa agttataaaa gcatatgtaa ttggaggtac
    17881 aaattctgtt tcaagagctg tagaaaagag tcttcctaat gcagaaagag tgagtgggaa
    17941 agatagaaat gagactaatg ctaaagttat agaaaagttt tacactgata caaatttaag
    18001 taatttatat gtaactaagg atggtagtaa aaatgagaat caactaatag actcgttagc
    18061 tgttggcgta ttggctgcaa aaaatgagtc tcctgttgtt ttggttggta ataaattaaa
    18121 cacaaaacaa agagatatac ttagtactaa aaaattaaat actataacac aggtaggtgg
    18181 aaatggaaat gaagaagctt ttgatgaaat taaaagtcta caagaaaaaa ctgtgtttga
    18241 agcaaaaact gttgaggaat taactgatat gataaatata gcaagtccta atgatattat
    18301 aaactttaaa ccaaaagaga atactgtaaa tgaagctttt agaatggtaa caaataaacc
    18361 aataactgtg aatataaagg gtgattgttc aaagactctt acagtagata tgcctaatgg
    18421 tgaagtgaat aactatgcta ctttggtaaa tgttatagtt agaaatattg gagaaggtgg
    18481 atttaataat catgatacaa tcactatatt gtcagttcgt gacaaaaatg gtagagttat
    18541 agaaaatact agaaattcag atatagatac gttgatgata ttagcttctg ctaatgatac
    18601 aaaattgatt aatgatggat atataggaaa acttatagat aattcatcta atagtgatat
    18661 aactaataat ggtactatag ataaaaaagt taatcaggtt gaagatttag aggctaaagt
    18721 agattcaatt gaaaaagcta tagattctat aagtcaaaaa gtcaataaaa tacaagatat
    18781 actagataag cttggatttc taaagaaatt tcttagctaa gaatatgtat atttaagtga
    18841 agtgtgtgtt gtaattgttc taaagttatg tagttaaaca ggtttatata caaatagatt
    18901 tatagataaa tatgtagttt agtatatatt tgaataagct ttggggaggt aacatgggga
    18961 ttggaattga atgtaacaaa aaaataaata aaagtattga caaaactaga ataaagaaaa
    19021 ataatct
//
LOCUS       DAMJVI010000075        18849 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.077, whole genome shotgun sequence.
ACCESSION   DAMJVI010000075 DAMJVI010000000
VERSION     DAMJVI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.077"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(318..710)
                     /locus_tag="PC545_002583"
     CDS             complement(318..710)
                     /locus_tag="PC545_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2457347.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEINTTLYM"
     gene            1277..1639
                     /locus_tag="PC545_002584"
     CDS             1277..1639
                     /locus_tag="PC545_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457348.1"
                     /translation="MKIRKSGFISIECIISIAILYVAVYLVSTSLYNCYSFISRNISD
                     REMLSTAKKYIEDEKYRIQNSKYELIEDKIEKNYINGYEINSRIEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1623..2330
                     /locus_tag="PC545_002585"
     CDS             1623..2330
                     /locus_tag="PC545_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457349.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFELGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNNEDEFREYFLGNNMIECIKKIKDTSYSS
                     LEELSLKVENNTIYDRGDFFEFDIESNVREKEFLKAIKVRVKIKNPWIGIDIEDYMDK
                     SNNNKIDDKTFNNYKASENNEIINQFKNESENIRNQEVVEFTEEDEEELKQNIIENIK
                     KNFDEKNLVQVYKYRGV"
     gene            2350..2769
                     /locus_tag="PC545_002586"
     CDS             2350..2769
                     /locus_tag="PC545_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457350.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNLENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIMLPKNAKIARSIEIIKFKSDG
                     SPQKASTIEVYNNRLRKTITITPVSGRVLLKEGKYET"
     gene            2759..3283
                     /locus_tag="PC545_002587"
     CDS             2759..3283
                     /locus_tag="PC545_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457351.1"
                     /translation="MKHKYGYLLLESVVSLSSMVIIILVLYSIFLSTINLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIMNVSNYEETFKKTTSIKCRYLDENNNEEGISNKEI
                     ILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSITNNGQYVNIKLKLSKCSQKYE
                     TDFKIKVWNFSESI"
     gene            3595..4152
                     /gene="efp"
                     /locus_tag="PC545_002588"
     CDS             3595..4152
                     /gene="efp"
                     /locus_tag="PC545_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDF2457352.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYEQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4244..4699
                     /locus_tag="PC545_002589"
     CDS             4244..4699
                     /locus_tag="PC545_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDF2457353.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFADAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4862..5572
                     /locus_tag="PC545_002590"
     CDS             4862..5572
                     /locus_tag="PC545_002590"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDF2457354.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGI
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINDDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5626..6696
                     /locus_tag="PC545_002591"
     CDS             5626..6696
                     /locus_tag="PC545_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDF2457355.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPADTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYNPFTLEESVN
                     IVKKVLVLFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKFGVLLKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6724..10278
                     /gene="smc"
                     /locus_tag="PC545_002592"
     CDS             6724..10278
                     /gene="smc"
                     /locus_tag="PC545_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDF2457356.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDVYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKTLEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELESNKLSGSEELSTLQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNIRDENINSEITELNKNIEIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHNKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KISNIKNEISHLELKRESLDKDVKNIKNEIDSHESKIKDLEKSIIIKSTSIKNVESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKSDDVRKDMEELDDLYNKNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEVYNSIVRDIKRISGENCELEEKNKQLEESLNYEE
                     HEIIKLQDSILTEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRSLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAQEITDII"
     gene            10305..11585
                     /gene="ftsY"
                     /locus_tag="PC545_002593"
     CDS             10305..11585
                     /gene="ftsY"
                     /locus_tag="PC545_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDF2457357.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDAEEEIEVEDSVDNLENLEETIFSG
                     LEEEVIDKVEDVEEKNEESVNNEGTEEIENFEKIEVEMDSNGKEVENISNNDSQEEII
                     EELENYDEIESKEIEDKEDKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELL
                     EDLEEILITADVGVNTTMDIIERLRDKIKQKGITEPIKVREELKSIVEDILTNENSTL
                     DIEPVPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWAN
                     RTNVDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKI
                     VDREFPEAKREVLLVVDATTGQNAVVQAKTFKEVADITGIVLTKLDGTAKGGVVLAVK
                     SEVDVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11688..12056
                     /locus_tag="PC545_002594"
     CDS             11688..12056
                     /locus_tag="PC545_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDF2457358.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12053..13405
                     /gene="ffh"
                     /locus_tag="PC545_002595"
     CDS             12053..13405
                     /gene="ffh"
                     /locus_tag="PC545_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDF2457359.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEVDVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIAKGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13443..13715
                     /gene="rpsP"
                     /locus_tag="PC545_002596"
     CDS             13443..13715
                     /gene="rpsP"
                     /locus_tag="PC545_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDF2457360.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13780..14007
                     /locus_tag="PC545_002597"
     CDS             13780..14007
                     /locus_tag="PC545_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDF2457361.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14115..14630
                     /gene="rimM"
                     /locus_tag="PC545_002598"
     CDS             14115..14630
                     /gene="rimM"
                     /locus_tag="PC545_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HDF2457362.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLAVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGMEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14639..15334
                     /gene="trmD"
                     /locus_tag="PC545_002599"
     CDS             14639..15334
                     /gene="trmD"
                     /locus_tag="PC545_002599"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDF2457363.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSMAKQ
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15519..15869
                     /gene="rplS"
                     /locus_tag="PC545_002600"
     CDS             15519..15869
                     /gene="rplS"
                     /locus_tag="PC545_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDF2457364.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16190..17086
                     /gene="ylqF"
                     /locus_tag="PC545_002601"
     CDS             16190..17086
                     /gene="ylqF"
                     /locus_tag="PC545_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDF2457365.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADRDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALRLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEVPENVKR"
     gene            17475..18767
                     /gene="brnQ"
                     /locus_tag="PC545_002602"
     CDS             17475..18767
                     /gene="brnQ"
                     /locus_tag="PC545_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2457366.1"
                     /translation="MGKTKDVIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFALSYVLTIRPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKAI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLNSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         7972 a         1762 c         3467 g         5648 t
ORIGIN      
        1 tagcagtatt aaatacacat taatatctat aaaatgacaa aataaaaaat actatttaaa
       61 tagatattaa aaaattgata ccaagtattt gtttgctata tgaataacaa tttatttagg
      121 agctttactt ggacactgta taataataaa gtaattgcta aaaacgtttg atgatttgga
      181 tgtattttta gaaattagaa aaatatatac ttactgacta atgataatct tatatgtatt
      241 ttagtttgag tgatttaaga aaagtgtttt gaatggggat tattttatag agtaaataaa
      301 tatataatag tagaaaatta catataaaga gtagtattta tttcattacc ttctttatct
      361 gttccgatag ggtcttgtag agatacttga gttttatttt ttttgttaga tctaatattc
      421 attaaaatct cattttcaat acatctagaa gcatatgttg ctaatcgagt tcctttatct
      481 atgttataag tttctatagc tttaattaat cctattgtac ctatggatat taaatcatcc
      541 tgatcttccg ttgaattatt atatttcttt gctatataag ctactaatct catgtttctt
      601 tcaattaatg tatcttttgc acttttatcg ttttctattt taaatttagt taaatactca
      661 atctcttctt caggggttaa gggtttttca aaagatttaa gagctgccaa taggtcaccc
      721 ccttttcaac ggtcacttta aattatatga aggagactta tccaagttga ctgttatcta
      781 ataaaataat atgcatattt ttagaattta aaacatatat ttgcaaactt ttgaatggaa
      841 tatgacataa agagtgggaa tttaaattta agtttttgtt gtatatatat gaaacaaggc
      901 gaagaaatta ttgatttata aagtataaat tggaattgag gtgtcaataa atataaaagg
      961 aaatagtatt tatatatgtt ttggtgttga agtaaaagat taatgcaaag cattagagtc
     1021 tataaaaata tgaatatagg aggtgtaatt ttacaatact taaaaaagta aatgtaatta
     1081 attgtttgta taaagttgat tacaagcaaa agtatcagtc atagttaata ctgttaagga
     1141 tgaaggtggt gtgttaattt atatatgtat gggaagaaat tttgattaca atgaaaaaac
     1201 ttacaaaaga tatttacgaa aaaacttata gttaaatgtt tatatataaa atattattta
     1261 ggggttgttt ttattattga aaataagaaa gagtggtttt atatcaattg aatgtataat
     1321 aagtattgct atattatatg tggctgttta tttagtttct acatcattgt ataattgtta
     1381 tagttttatc agtagaaata tatctgacag agaaatgtta agtacagcaa aaaaatatat
     1441 agaagatgag aagtatagaa tacaaaatag taagtatgag ttaattgaag ataagataga
     1501 aaaaaattac ataaatggat atgaaattaa cagtagaata gagcaaattt tagattatta
     1561 tcaatgctat gaaataaata tagagataaa aaatgaattt aaaaaactga ggtttaatag
     1621 ctatgttact agaaaataaa ggctctatat taataataac attgataata ttttctatta
     1681 taagtactgt gtgcattatg tgtataggtt taatttatac acataataat atgtttgagt
     1741 taggatacaa agacattcaa ctaaaagaaa tttctttaag tggaatagaa atatcagtaa
     1801 gtaatgtact ttcttgtgtt gaggaagcta ttgatatatc taataatgaa gacgaattta
     1861 gagaatattt cttaggaaat aatatgattg aatgtataaa aaaaataaaa gatacatctt
     1921 atagtagttt agaagaactt tctttaaaag tagaaaataa tactatttat gacagaggag
     1981 atttttttga atttgatata gaaagtaatg ttagagaaaa agaattttta aaagcaataa
     2041 aagtaagagt gaaaatcaaa aatccatgga taggaattga tattgaagat tatatggata
     2101 aatctaataa caataaaatt gatgacaaaa cattcaataa ttataaagca tctgaaaata
     2161 atgaaattat aaatcaattt aaaaatgaaa gtgaaaatat aaggaatcaa gaggttgttg
     2221 aatttacaga agaagatgaa gaagaactta aacaaaacat aattgaaaat ataaagaaga
     2281 attttgatga aaaaaaccta gtacaggtat ataagtatag gggtgtataa ctttgaaaaa
     2341 agaaggaatg tgactgtaat agaattaata gtatctatca gtataatact cttgatagtt
     2401 acattatctt ttccaaaaga taatcttgaa aatcataaaa taaatttatt tgcacgtcaa
     2461 ctttgttcag atataaggta tgttaggcga attaatatgt ttagcaacta caatacatat
     2521 atatattata taaatcaagg taaacacgaa ggttatgtat tgagaaaaga tggtaaaaac
     2581 attaaatcaa taatgctacc taaaaatgca aaaatagcta ggagtattga aataataaag
     2641 tttaaatctg atgggtcgcc tcaaaaggcg tctacaatag aggtatataa taatagatta
     2701 agaaaaacta taacaataac acctgtaagt ggaagagtac ttctgaaaga gggaaaatat
     2761 gaaacataaa tatggttact tgcttttgga aagtgtcgta tcattgtctt caatggtgat
     2821 aattatacta gttttatatt ctatattttt gtctacaata aacttaaagt taaaagtaga
     2881 agataagata gaactacaac aacaaagtct tgaaatcata aaatctatgg aaggaattat
     2941 tagcaattct atgggaataa tgaatgtatc taattatgaa gaaacattta aaaagacaac
     3001 atctataaaa tgtagatatt tagatgaaaa taataatgaa gaaggcataa gtaataaaga
     3061 aattatattg aatgaaagac gaaataagtt atttgtaaat tctttaaatg gcgaaagtag
     3121 tcaagcaggt ggttatgaaa ttggagatta tgtagatgaa atgtatgtat caattactaa
     3181 taatggtcaa tacgttaata ttaaattgaa attatcaaaa tgtagtcaaa aatatgagac
     3241 tgatttcaaa ataaaagtat ggaattttag tgaaagtata taaaaataca taaatttaag
     3301 agaaagaaat gacatcaata agtcatataa aatataatta ttgagaacta tataactaaa
     3361 taatatcaat atgtaaagtg tatagattaa gaatatagag gataataata aaaatataga
     3421 ataagtaatt tgccaaatac ttgtttatat tgtataactt atgatattat gatattagta
     3481 aaaatttgta atcctgactt gacagaattc aagtaagaga tataatttta aaggcatagt
     3541 aatgtaaaat ttactaaaaa ataagaaatt tattttaata tggaggaaca aaaaatggta
     3601 tcagcaggtg attttagaaa aggtgttaca tttgaaaaag atggacaacc atgtttagta
     3661 gttgattttc aacacgttaa gccaggtaaa ggagctgctt tcgttagaac taaatacaaa
     3721 aacttaaaaa caggagctat aagagaagaa agtttcaatc caagtgaaaa attccctaaa
     3781 gctgttatag atacaagaca aatgcaatat ctatacaatg atggtgagtt atattatttt
     3841 atggatcaag aaaattttga acaaatacca ttaaactatg agcaagttga agatgcaatt
     3901 aagttcttaa aagaaaatga agttgctaca ataagatttt accaaggaca accattccaa
     3961 gtggaagcgc caaactttgc agagctagaa gttacagata cagagccagg cataaaaggt
     4021 gatacagcaa gtaatgtaac taaagcagct acagttgaaa ctggagcagt tgttcaagtt
     4081 ccattattta taaatactgg agataaagta aagatagata ctagaacagg tgaatattta
     4141 tcaagagtat aaaaatttat gtatataaaa gcttatatgt cttaataata taagatatat
     4201 gagcttttag tttatatata aaaaagaaag cgaggggtgt tacatgaaac atttatatga
     4261 agaagttgca tacttaaaag gcctagcaga aggactagaa ataagtgctg aaagtaaaga
     4321 agggaaaatg atacataaaa tagttgatgc attagaagta tttgcagatg ctattgtaac
     4381 attggatgaa gaacaagaag aacttcaaga ttttgttgaa tctatagatg aagatttagc
     4441 agatttagaa gaagatattg ctgacatgga agaagatctt tatgaagaag atgacgatga
     4501 ggatgatgag gacttcagct atatagaaat ggaatgccca aattgtggag agttagtaga
     4561 aatagacgaa gatttgttat atgatgatga agtagatgtt gtttgtcctg attgtaaggc
     4621 tgtgatatta tcatcagaag atgattgtga tgatgattgt acatgtggtg gttgttcaag
     4681 ttgtgatgat agagagtaaa aattcataag aattaaaact gcctatgatg cctatgagat
     4741 attttgcttc ataggtagtt ttatttttat gaggaagggt attatgtatt ttggttttgc
     4801 aatacttgga caaaaatagt acactagaat ttatagaata ggattttgga gggatgtaaa
     4861 tatgaaaata agtaaaaaat tactagataa tatacaaaga tttgaaaatg ttataaatta
     4921 taaatttaaa aataaagaat atatactaga agctcttact catagttcat attctaatga
     4981 aaataaaaaa tataatttta acgaaagatt ggaattttta ggggattcgg ttttaggcat
     5041 agtgataagt gattatttat ttaatgaaga agctaatctg cccgaagggg aactaactaa
     5101 acttagagca aatatagtct gtgaagattc attaagtgaa gtagctaatg atataaattt
     5161 aggtattcac atgttattag gtagaggaga agaagcaaca ggaggaagac ataggacttc
     5221 aatattagca gacgcatttg aagctgtaat agctgctata tatcttgatg gaggttttga
     5281 aagtgcaaga cagtttatac ttcatcatat ggaaaatata atttatgatt caagaaaagg
     5341 aaatatattt agagactaca aaacgcatct tcaagaggtg ttacaaggga atggagaaaa
     5401 taatatctgg tacaggctta tagaagaaaa aggaccagac cataataaaa gatttgtaat
     5461 ggaagttgga attaatgacg atgtacttgg tataggagaa ggcaagagta aaaaagaagc
     5521 tgaacagtta gctgctaaaa tagctttaaa gaaaaaatta tgggaaaaat aaatataata
     5581 tgtaaaaata taaaaagaaa tatttatatt agaaggatgt tgtagatgaa aaagagaatt
     5641 atacctatat ttgttcctca caaaggatgt cctcatgatt gcatattttg taatcaaaaa
     5701 aaaataacag gtgtgagtac tgatgttact agtgaagatg caagaaatat aattgaggaa
     5761 tgtttagaga ctatagataa agatgcagat gtagaaatag cattttttgg tggtagcttt
     5821 actgctatag atattgatat acaaaaaagt ttattatcag ttgctaaaga atatgtagaa
     5881 aaaggtcttg taaaagatat tagaatgtct acaagaccag attgtattga tgaagatata
     5941 ttaagtatgt taaaagaata taagacaagt ataattgaac taggagtaca atccttagat
     6001 gaaaaagtat tattagatag tataagaggt catcaatctg aaattgtata caagagttca
     6061 aagatgataa aaaatagtgg aatcaaactt ggattacaga tgatgattgg tcttccagct
     6121 gacacagaag aaaagtgtat atttacagca aaaaaatttg tagagctaaa accagattgt
     6181 gttagagtct atccaacttt ggtagtaaaa gatacaggtc ttgaaaagtt aatggaacaa
     6241 aataaatata atccatttac ccttgaagag agtgtaaata tagtaaaaaa agttcttgta
     6301 ctgttttatg ttaataatat caatgtaata agagttgggc ttcaagctac tgatgatata
     6361 caaattggaa aagctgtttt agcaggacca tatcatcctg catttagaga gttggtagaa
     6421 gcagatatga taaaagatta tttagaattt gttattttgc aaaataaaaa tataaagcaa
     6481 atgttggtca aatctaataa aaagaatatt tctaaaatca taggaaataa aaaaacaaat
     6541 gttaaatata tggaagaaaa atttggtgtg cttttaaaaa cacaagaatc agatttagat
     6601 ataaatcaat tagaaattgt gttagatggg aaaagtttaa ttagtgctaa tatgagagac
     6661 attcatagaa aattgtatga catttatgac ctttagtttt aataatgaag ggagagaaaa
     6721 gatttgtatt taaaaagact agagttaaag ggatttaaat cctttccagt aaaaacagat
     6781 attattttta aagaaggaat aacagccata gtaggaccaa atgggagtgg aaaaagtaat
     6841 atatctgatg ctgtaagatg ggtattagga gaacagagta ttaagagtct tagaggtgat
     6901 aaactcgaag atgtaatatt tgcaggtaca gatacaaaaa aacctatgaa ttattgtgaa
     6961 gtggctctta ctattgataa ttctgaaaat caattggaat tagattttac agaagtaact
     7021 ataagaagaa gggcttatag aaatggtgaa agtgaatttt ttctaaataa taaaagttgt
     7081 agattaaaag atataaaaga agtcttttta gatactggta ttggaaaaga tggttattca
     7141 atcatagaac aaggaaaagt agatgaaata ttaagtaata atcctttaag cagaagaaaa
     7201 gtttttgatg aagcttgtgg catatcaaaa tatagatata aaaaacagga agcagaaagg
     7261 aatttaagta atacaaagga aaatttagaa agaatagatg atgtatatat agaaattgaa
     7321 aatcaattaa agcctctttt taatcaacag acaaaggcaa aaaagtactt ggaaataagt
     7381 gaaaaattaa agacacttga agtaaatagc tttataagag aaattgaagg aatagaaaaa
     7441 gaattaagtg aggttaatga acatcgtaag gttattgaaa aggagctcaa tgaaaaagaa
     7501 gaacaaaaaa atgttgtaga gaaaaagcaa gaagatatta ataaagaggt agaggtatta
     7561 caagatgtaa ttgaaaaatc tgtagattat ataaattcaa ttaaaggagt tatatcaaaa
     7621 aaagagtccc aaataaattt aattaaggaa agaataagaa attttacgaa tgaaattagt
     7681 agaaaaaatt tagaaataaa tgatataaaa gagaagctaa atgaaaataa gcaatacata
     7741 aaagaattag aaagcaataa attatcaggt agtgaagaac tatctacctt gcaggaaaat
     7801 ataaaagtat tggaagcaag taaagataaa caaaaaatta agttagaatc cctaaataat
     7861 gagatagaat tattgaaaga aagtataatt gatatattaa ataaaaaaca agagtttagt
     7921 aataagctgt ccactttaaa cgcaaataaa gaaaatatga atatcagaga tgaaaatata
     7981 aattcagaga taacagagtt aaacaaaaat attgaaataa aatcatctga attagatact
     8041 ataaataaag aatttaatat gcaaaatgaa aatttaaaga atgtaaataa tagacataaa
     8101 gaattatcaa ctaatttgca ggattcaatt agtgaacaca ataaactaga agatgaaata
     8161 cagaaaagta aatataattt aaatggatat aattcaaagt taaatgtcta tatagatatg
     8221 gaaaatcatt atgaaggatt taatagagga gtaaaagaag tactaaaaaa taaaaattta
     8281 aaaggagttc atggagcatt aggacagatt ataaatgtac ctgaaaaata tgagaaatct
     8341 atagaagcag ctcttggtgc ttatatgcaa aatataataa ctgataatga gtttagtgct
     8401 aagtctgcaa taaactatct aaaacagaat aatttaggta gagtaacatt tcttccatta
     8461 aatataatta aatcaaataa aataagtcta ggaaatttaa aagcaaatac taagttcata
     8521 ggaatagcaa gtgatttaat tacatttgat gaaaagtata gaaatataat tgaaaatata
     8581 cttggaagaa caatacttat aaataatata gatgagggca taaaatttgc aaaagaaact
     8641 ggtcataggt ttaaaatagt aactttagat ggtgaaatct taaatccagg aggttctttg
     8701 actgggggaa gtctaaaaac taatgggaat atactatcca gaaaaagata cattaatgaa
     8761 tatacagaaa agataagtaa tatcaagaat gaaatttcac atctagaatt aaaaagagaa
     8821 tctttagaca aagatgtgaa aaatatcaaa aatgaaattg attcacatga aagtaaaata
     8881 aaagatttag aaaaaagtat aataataaaa agtacaagta taaagaatgt tgagtctgaa
     8941 attgaatcgt taaaaggtag tattactaaa ttagaaaatg agaagaatga tttaaattct
     9001 aacctaaatt atacacttga aaaaagtgat gatgttagaa aagatatgga agagttagat
     9061 gatttataca ataaaaataa agaaaaaatt gatgcattga atgaggaaat aaaaagatac
     9121 aatgatttat atgataaaga aaaatctgaa tttgatgaat tgaacttaag tttagtgaag
     9181 aaaacagaag tttacaatag tatagttaga gatataaaaa gaatatctgg tgaaaattgt
     9241 gagctagaag aaaaaaataa acaattagaa gagtcattaa attatgaaga acatgaaata
     9301 attaaattac aagattctat actcactgaa gaaaaagaaa aagaaaattt aacaaaacaa
     9361 ctaggggata gtaatagaaa tcttgaaact agaaaaatag caaaagatga tttaaaaaat
     9421 agttttgatg aaattaataa agaattaaaa actatagata gacagcatat agaacttaaa
     9481 gaaagtttat ttaaagttgg tggaagatta gaaagattaa aaactagtca agacacatat
     9541 ataaataagt tgtttgagca atatgatatg accttagtac aagcattgga aattaaagat
     9601 gaagatttgg atatagatag aaaattctta gaaagtctaa agagagaaat aagaagtctt
     9661 ggaaatataa atatagactc tataaaggaa tatgaggaaa taaaagaaag atacgatttt
     9721 tatagcgaac aaaagcaaga tttagaagag tctatggaag agatagaaaa acttatacat
     9781 actttagaag aaaatatgaa atctgaattt gaaattaagt ttgaagaaat aagtaaaaat
     9841 tttaaatatg tatataagag attatttggt ggaggttgtg gagaattaac tattttagat
     9901 aaagaaaatt tattagaaag tgatatacta ataacagctc aacccccagg taaaaaaatg
     9961 aaaaatttaa acctattatc tggtggagag aaagctttaa cggcaataag tatattattt
    10021 gctatattga ttacaaagcc aacaccattt tgtatattag atgagattga agcgccactt
    10081 gatgacgcaa atatatttag atttggagaa tttttgaagg atttatctaa agagactcaa
    10141 tttatatcag taactcatag gagaggaact atggaagctg ctgattatat ctacggagtt
    10201 acaatgcaag aaaaagccat ttcaaaagtt attagtttaa aattaaaaga agctcaggaa
    10261 ataacagata ttatataact tagattgaca taggaggaat aacagtgtta aaaaaattat
    10321 ttggttttgg aaaagacaag gaaaaggaaa tagaaaaaaa agatgctgaa gaagaaatag
    10381 aagttgaaga cagtgttgat aatttagaaa acttggaaga aactattttt tcaggacttg
    10441 aagaagaagt gatagataaa gtggaagatg ttgaagaaaa aaatgaggaa agtgtcaata
    10501 atgaaggcac tgaagaaatc gaaaactttg aaaaaataga agttgagatg gatagtaatg
    10561 gaaaagaagt agaaaatatt tcaaataatg attctcaaga agagataatc gaagaattag
    10621 aaaattatga tgaaattgag tctaaagaga tagaagataa agaagataag aaagttaact
    10681 tgtttgaaag actaaaacaa ggattgacaa aagcaaagca aggaataact gatagaatag
    10741 atgaagtttt aaaatcatat acaaagatag atgaagaatt acttgaagat ttagaggaaa
    10801 ttttaataac agcagatgtt ggtgtaaata caactatgga tataattgaa agactaagag
    10861 ataaaataaa gcaaaaaggt attactgaac caataaaggt aagagaagaa cttaaatcta
    10921 tagttgaaga tattcttaca aatgaaaact ctacactaga tatagaacca gtaccatgta
    10981 taatattaat ggtgggtgta aatggggtcg gtaagactac tacaataggt aaactagcaa
    11041 atagatacaa aaaagatggt aaaaaagttt tattagcagc agcagataca tttagagcag
    11101 cagcaacaga gcaattagaa atttgggcaa atagaaccaa tgtagacata ataaaacatc
    11161 aagagggagc agaccctgga gctgtagtat ttgatgctat aaaagctgca aaagctagaa
    11221 aaacagatgt attaatatgt gatacagcag gaagattaca caataaggct aatcttatga
    11281 atgaacttgg aaaagtattt aaaatagtag atagagaatt tccagaagca aaacgagaag
    11341 tacttcttgt ggtggatgct acaacaggtc aaaatgcagt tgttcaagca aagacattta
    11401 aagaagttgc agatataaca ggtatagtac ttacaaaact tgatggaact gcaaaaggtg
    11461 gagtagtcct tgcggtaaaa tcagaagtag atgtacctgt aaaacttatt ggagttggag
    11521 aaagtgtaga agacctacaa gatttcaatg caaaatcatt ttcagatgca ttatttggta
    11581 actaatgttc ttttaaaaat aatggttgac aagagtgttg ttatgatata aaatacaata
    11641 gtgtaaagta aataaccttt acagtatact gctaggaagt gtaagatatg aatatagaaa
    11701 aaatggtcga aattggattg ttatttgaac agtataaaga attattaact gacaagcaga
    11761 aagaaatagt tgctctatac tatgaagaag attattcttt aggtgaaatt agtgaaaatc
    11821 tgaatgtatc aagacaaggt gtttatgata ccttaaagcg ttcagaaaaa atactaagag
    11881 attatgaaga aaagttacac ttagtatcaa agattcaaga gcaggaaaaa aatattaaga
    11941 ttataaaaga taaaattatt gacattaaag aagatttatt gcacaataga gattgtgcta
    12001 atttaatccc taagctagaa aatatagaag atgtatgtag ggagatgata aaatgatatt
    12061 tgaaggattg tctgataaac tacaaggggc atttagtaag ttaaaatcta aaggaaaact
    12121 tacagaagta gatgtaaaaa atgctatgag agaagttaaa cttgctcttc ttgaggcaga
    12181 tgttaacttt aaagtagtta aagatttcat aaaaaaagtt caagagagat gtgttgggca
    12241 agaagttatg caaagcttaa ctccagctca gcatgtaatc aaaattgtaa atgaagagct
    12301 tacaagttta atgggagatg tacaaagtaa agtaatgatt tcctcaaagc ctcctacaat
    12361 tttaatgatg gttggattgc aaggtgcagg taagactact acagcaggaa aacttggagg
    12421 atattttaaa aagcaaggta aaaaacctct attagtagct tgtgatattt atagaccagc
    12481 agctataaag caacttcaag ttgttggaga aaaattggat atacctgtat tcaatatggg
    12541 agataaagaa aatccagtaa atattgcgaa agcaggttta agccatgcga ttaaaaatgc
    12601 taatgattta gttattatag atacagctgg tagacttcat atagatgaag tacttatgga
    12661 tgagttaaag tctattaaat cagaagttaa gccacatgag atacttttgg ttgtagattc
    12721 tatgacaggt caagatgctg taaatgttgc agaaagcttc aatgaggcac ttggtgttga
    12781 tggagtagta ttaactaaat tggatggaga tactagagga ggtgctgcac tttcaataag
    12841 agcagttaca caaaagccta taaaatttat ggggatggga gaaaagctag atgatataga
    12901 acctttccac ccagatagaa tggcatcaag aatattaggt atgggagatg tcttaagttt
    12961 aatagaaaaa gcacaagaaa atatagattt agagaaagct aaagaattag agcaaaaaat
    13021 taaaaaacaa gagcttgatt ttgaagattt cttacaacaa atggaacaaa tacaaaatat
    13081 gggacctctt gacaagatac ttggtatgat acctggtatg ggaaatgtaa aagaccaact
    13141 tggagatgta gatttgaatg gcaaagaaat gaagagaaca aaagccataa ttcagtcaat
    13201 gactacatat gagagaagaa acccaggagt attaaatgct tcaagaaaga aaagaatagc
    13261 taaaggcagt ggaactagtg tacaagatgt aaatagactt ataaaacaac ttaatgaaat
    13321 gaaaaaaatg atgaagatgt ttgctggttc acaaaagagt atgaagaaaa ggggcggctt
    13381 aggcggttta ccttttttta aataaaatat aaaaaagtaa aaattattcg gaggtgactg
    13441 taatggcagt taaaataaga ttaaaaagaa tgggagctaa caaaaaacct ttctacagaa
    13501 tagtagtagc tgattcaaga gctccaagag atggaaaatt tatagaagaa ataggatact
    13561 ataatccaat ttctgaacct aaacaagtaa gaataaatga tgaaaaagct ataaaatggt
    13621 tagctactgg tgctcagcca acagaagtag ttaaaaaatt acttgtaaaa aacggagtaa
    13681 tagaaaaatt cgaagcttct aaacaagcaa aataattttt tcttggcaat tagtttaagt
    13741 aagggctcat tgctaaggtt actttaggag atgaaatata tgaaagagct agttgtagat
    13801 atagctaagg ctctagttga taatcctgac tcagttgttg ttgaggaatt tgaagataat
    13861 gatggtatcg tcttaaagct tacagttgct caagaggata tgggtaaggt tattgggaag
    13921 cagggaagaa tagctaaagc tataagaact gtcgtaagat ctgttgcaaa tagagaaaat
    13981 ataaaagttt ctcttgagat agtataaaat aggaggatta ggtataatgc ctagtccttt
    14041 ttatataaat gcgtagttta agattttatg atgatatgac cacataagat taaatttttt
    14101 aagaaggtga taacatgaaa gaaaaattaa ctcattttaa ggtaggtcaa atagtaaata
    14161 cacaaggttt aaaaggtgaa gtaagagttt atccgcttac tgatgatata gagaggtttg
    14221 atgaattaaa agatttctat ctgggaaaag atttaaataa taaattggct gttgaaaaag
    14281 taagatacaa aggttctatg gttataatga agataaaagg catagacagc atagagaaag
    14341 cagaaaaact aaaaaataaa tttatgtatg tatctagaga agatagcaga gagcttgaag
    14401 aaggcgaatt ttttatagca gatatgattg gaatggaagt tttaactgta gatggaaaat
    14461 atgtaggaat actagaagat gttcttcaat actcagcaaa tgatgtctat gtagtaaaag
    14521 gtgaggaaga taaagaattt atgataccag ctataaaaaa atttgtacct acaatagata
    14581 ttgatgaaag aaaaatgatt atagacccta ttaagggaat gatagattag gtggtaatat
    14641 gagatttcat ataatgacac tttttccaga aatatttaat tcgtacatgg atgaaagtat
    14701 aatgaagaga gcagttgaaa aaggtataat tgaggttcat atatataata taagggattt
    14761 ttctaataat aaacataaaa aagtagatga ttatccattt ggaggaggag cggggatggt
    14821 aatgactcct caacctatat atgacacata taagcatata ataacaactc ataatataaa
    14881 taacccaagt gtaatttatc ttactccaaa aggtaaggta tacaatcaat ctatggcaaa
    14941 gcagatgtct ttaaaagaag atataatact actatgtggt cattatgaag gaatagatga
    15001 gagaatcatt gatttaatag ttactgatga aatttcaatt ggagattatg tattaactgg
    15061 gggagagctc ccagcactta taatgataga ttctatatcg agattaatac caggtgtatt
    15121 aaaccaagaa gaatcatttg aagaagaatc atttaaagac aacttattgg aatatcctca
    15181 ttacactaga cctagagatt ttgaaggatt aaaagtacca gaagtgcttt tatcagggaa
    15241 tcataagaag atagatgagt ggagaagaga agaatctata aggataacca aagaaagacg
    15301 atttgattta tataaaaagt ctaatgaaaa ataaaaatat aatgaaaaat taatatatat
    15361 gtatacaata aagtacctat aaatggaatt aaaaacataa aagtgcttgt aataactatt
    15421 gaagtatagt ataatataaa atgttgaaaa tacgggcgtt cctctgttta cactggtcgg
    15481 gtagttaaga acgtctatga tatctaggag gaagaaagat gaatgaaatg ctaagagcaa
    15541 ttgagcaaga acaattaaaa aatgaagttc ctaattttgg acctggggac acagttaaag
    15601 tacacgttag aattatagaa ggaaaaagag aaagaataca agtattcgaa ggtgtagtat
    15661 taaagagaca aggtggagga gcaagagaaa ctttcacagt tagaaaaatg tctttcaatg
    15721 ttggtgtaga aagaactttc ccagttcact ctccaaaaat agaaaaaata gaagttacta
    15781 gaaaaggtaa agtaagaaga gctaaactaa actacttaag aggtagagta ggtaaagctg
    15841 ctaagataaa agaagctaga aataaataat taaaaacttt acttataaat ggggggctga
    15901 tttatcagtc ctctttctat tttaatgaaa attacaacaa ataattatac tagaaataaa
    15961 tagaaatatt tgttgaaaat atagattatt tatctatgaa tttatagctt ttaaaatgaa
    16021 aaataaaatt ggtaaatatt gcattatatt ttgtaacgat attaaaaata agagaacagt
    16081 attatgtaaa gtatgacgtt aaaaagatga caatattata gtttgtgaag atatattttt
    16141 taagtggaat taggatataa taatataata ttaataagga gtgatacatg tgtcaaatga
    16201 atacgaagag tatcttatgg atgacaatct acatataaac tggtatccag gtcatatgaa
    16261 aaaaactaag gagttagtaa aaaataactt aaaattgatt gatgtagtaa ttgaactttt
    16321 ggatgctaga atcccgttta gtagtaaaaa tccagacata gacaggcttg taggagataa
    16381 gccaagagtt gtcgtactta ataagagtga tatggcagat agggataaat tgaatcaatg
    16441 gattgaatac tataagaaaa ttaatatcaa agctatacca gtagatacaa taaaaggtgt
    16501 tggtataaat aagataatcg aagaatgtaa aaatgtaact agagaaaaaa tgagttcttt
    16561 aaaagacaaa gggagaaaag aaagagctat acgaataatg atagttggcg ttccaaatgt
    16621 tggtaaatcg tctcttataa acaaattaac aggtagaaaa agcactcaaa ctggagataa
    16681 gccaggagta acgaaaggga aacaatgggt tagattaaag ggaaatcttg aacttttaga
    16741 tacgcctgga atactttggc ctaagtttga agaccaagaa gttgcattaa atttagcatt
    16801 tagtagagct ataaaggatg agattttaga tactgaaact ttggctttaa gacttataga
    16861 aaaacttatg aaaatagaac ctgagaagtt gaaagccaga tataagttag attgtctagg
    16921 agaaactcca attgaaacta tggatatgat tggtcataaa agaggattta taacaggtaa
    16981 aaaagaacta gattatacac gcatagccac aactgtatta aatgaattta gagatggaaa
    17041 aataggaaat ataacattgg aagttccaga aaacgttaaa agatagattt taaaacaaaa
    17101 tcaaaaattt aatgttaaaa atttaaaaaa gccttttagc aaaggctttt ttattttttt
    17161 gaaatataga caaatatctc catataaaag tataaattag gttagaaagc ctttaaaaat
    17221 attaatttaa gacaaatata cctaatattt tgaaaattaa aatgagtgtg attattaaaa
    17281 attttagcat attgacttgc ttgtttcgta taatatataa taagcacaaa taaatacaat
    17341 atattaaaaa tttaatttat ttaattttat taatttaata ataagttctt attgtttaat
    17401 tcaacttact tatgcaagcg taaacaatcg agacatttta ttaatttaat aaggttaaaa
    17461 agggggattt tataatgggt aagaccaagg atgttatagt atttggattt gcgttatttg
    17521 caatgttctt tggagcaggt aacttaatat ttccacctta cttgggaata attacaggtc
    17581 cagaatggtt aattgccttc ttaggattta cgtttgctga tgctggattg gcacttctag
    17641 cagtaatggc tacagctaaa ttcgatggaa acgtagttga aatgtttaaa agatgtggta
    17701 tgaagttagg gattcttata ggttgtgcag atattttatg tataggacca ttcttagcta
    17761 taccaagaac aggtgcaaca acttatgaaa tgggtataat gcctttattt ggaacttcaa
    17821 ttccagtgtt attattctgt atactttttt ttgcgctttc atatgtacta acaataagac
    17881 cttctaaggt cgtagatata gttggacagt ttttaactcc agctctttta atagctttag
    17941 catttataat tataaaaggt attatatcac ctcttggaga tatagtagat aaacctatga
    18001 tacctaatgt atttgcagaa ggtataggtc aaggatatca gacaatggat gcttttgcag
    18061 caatagcact tgcatcagtt ctaatagtat ctttaaatga taaaggatat tctactatta
    18121 gtgacaagtt aaaaatgatt ggtaaggcgg gtgttcttgc ttgtggagga ttagctctag
    18181 tttatggagg attatgtttc cttggagcaa ctgtatcaac aatgtatgga acagatgcag
    18241 tacaatcaca agtaatagta aatataactg aaggattgct tggaaatgtt gggaaagcga
    18301 ttttagcagt agtagtatca ttagcatgtt taactacatc tataggtctt acatctgcca
    18361 caggtcaata tttctctaga cttacaaaag gtaagttgag ttatgaaaaa atagtattag
    18421 ctgtatctgt attcagtgca gtagtagcat cttttggtgt tggaactata ataaagatag
    18481 catctcctat attaagtata gtttatccac catcaattgt tttaataatt ttagctttct
    18541 tcaatgaaaa aattaagaat gataatgtgt acaaaggagc agtatatatg tcattgcttg
    18601 taagtatact aacagttata tctagttatg gagtagctgt tccagtagta aactctttac
    18661 ctcttaattc attaggattt aactgggtgg tgcctgttat aatagctgga ataattggca
    18721 attttatacc atcaaagagt caatctaata cacttggaac aaactaagtt ttatttaatt
    18781 aaaatataat caattgtaaa taaatgagtt aactaaaagt aaaaggagtt atttgtacaa
    18841 ataactcct
//
LOCUS       DAMJVI010000076        18591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.078, whole genome shotgun sequence.
ACCESSION   DAMJVI010000076 DAMJVI010000000
VERSION     DAMJVI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.078"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            182..1057
                     /locus_tag="PC545_002603"
     CDS             182..1057
                     /locus_tag="PC545_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDF2457367.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGNEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDEVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            1075..2565
                     /gene="cls"
                     /locus_tag="PC545_002604"
     CDS             1075..2565
                     /gene="cls"
                     /locus_tag="PC545_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDF2457368.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGVGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKITEIVELEKESIKQN
                     KLLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKRLIEDIREAKDHIH
                     LEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNRKFFREMREVGIEI
                     AAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGKNDKFGYWRDTHIR
                     IKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNVGESMIQVVASGPDSDWEDIHY
                     AYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFPKIADHKIVNIASY
                     SYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSFMLNFEVNAFIYDE
                     EVIRVMTDDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPIL"
     gene            2758..3042
                     /locus_tag="PC545_002605"
     CDS             2758..3042
                     /locus_tag="PC545_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDF2457369.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            3075..4703
                     /gene="groL"
                     /locus_tag="PC545_002606"
     CDS             3075..4703
                     /gene="groL"
                     /locus_tag="PC545_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDF2457370.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEDRVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVVKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDVGMPGMGGGMPGMM"
     gene            4965..5705
                     /gene="larB"
                     /locus_tag="PC545_002607"
     CDS             4965..5705
                     /gene="larB"
                     /locus_tag="PC545_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HDF2457371.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYNHAEYEEASK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLASMINKL"
     gene            5789..6523
                     /gene="larC"
                     /locus_tag="PC545_002608"
     CDS             5789..6523
                     /gene="larC"
                     /locus_tag="PC545_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HDF2457372.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLRMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDKSTLNENVKSKAKKIFMV
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATIEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDELEFSPKQI
                     GYGIGHKKFEVPNILRTVLGEKKKKN"
     gene            6553..6972
                     /locus_tag="PC545_002609"
     CDS             6553..6972
                     /locus_tag="PC545_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HDF2457373.1"
                     /translation="MSSEIYSYLYEKILDEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGKLVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      7032..7133
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7354..8889
                     /gene="guaA"
                     /locus_tag="PC545_002610"
     CDS             7354..8889
                     /gene="guaA"
                     /locus_tag="PC545_002610"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDF2457374.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIEKVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            complement(8966..10012)
                     /locus_tag="PC545_002611"
     CDS             complement(8966..10012)
                     /locus_tag="PC545_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDF2457375.1"
                     /translation="MPAYKDEQRKSWYASFYFTDFDGDRKKKIKRGFKTKKEALEFER
                     EFLNKSKMSTDMSFESLIEEYMHDMSSRLKLSTLETKKYLINFRILPFFKSLKINEIT
                     ATHIRRWQNELLKSDYSQTYIKTINNQLVAVLNYAVKYYNLPSNPAHLAGSIGKKNAD
                     EMNFWTLEEFKKFIEFEKKSEPRLAFEILFWTGLRLGELLALTPKDIFENKISVEKSY
                     IRLNGEDIVSSPKTPKSKRVVPIPHFLYNNIKDYLSKLYDLKDNERIFKFAKSYLSKE
                     LDRCCKLSNVKRIRVHDLRHSHASLLVNMDVNILTIAERLGHEKVETTWNTYSHLYPN
                     KQLEVAQKLDNLNI"
     gene            complement(10089..10304)
                     /locus_tag="PC545_002612"
     CDS             complement(10089..10304)
                     /locus_tag="PC545_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457376.1"
                     /translation="MLKKLRKKKRMTQLELAEKMRRNRSYISKLENQEYKDIGISTIL
                     DLSIALEEDFLELCKYYKLQEIKRRGK"
     gene            complement(10738..11139)
                     /locus_tag="PC545_002613"
     CDS             complement(10738..11139)
                     /locus_tag="PC545_002613"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457377.1"
                     /translation="MQTLAEIIKEYRSEHNLSLREFSNLCGVSHTYIDKIEKNRDPRN
                     GKAVEPTLDMLEKMSFGLNLTLKELLTKLGKIQPNTQQDDELLKKVNSKEDNEVNELI
                     NRLASLDSDDKNAIKKMIDNAYYKAVNTKKE"
     gene            11304..11516
                     /locus_tag="PC545_002614"
     CDS             11304..11516
                     /locus_tag="PC545_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457378.1"
                     /translation="MKNNLQYIRKESRISQKKFADKIGISRPYLSRIENGKVNPSLEI
                     AHKISLETGKTINEIFFDFTVNHSKL"
     gene            11756..11893
                     /locus_tag="PC545_002615"
     CDS             11756..11893
                     /locus_tag="PC545_002615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457379.1"
                     /translation="MQREQTTLRIPEDLHKALIDLSSDIGMPITSIIIIACWLYISKI
                     N"
     gene            complement(11879..12190)
                     /locus_tag="PC545_002616"
     CDS             complement(11879..12190)
                     /locus_tag="PC545_002616"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457380.1"
                     /translation="MTTITVRIYTPLNENLEKISYQTGILKSSLILYAINDIIRNSKV
                     NELQSISYKSDDTVRSTLRIPGVLKELLEKTAKENNLSINSLINNVVHSFCISDWLIY
                     L"
     gene            12311..12481
                     /locus_tag="PC545_002617"
     CDS             12311..12481
                     /locus_tag="PC545_002617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457381.1"
                     /translation="MEKVRMTVRLVPKLNQYVQNIAKECGKSKNSIIVDACWEFIEKI
                     KKENKFNVESEE"
     gene            12484..13122
                     /locus_tag="PC545_002618"
     CDS             12484..13122
                     /locus_tag="PC545_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457382.1"
                     /translation="MNNLQIFEKLEFGQIRMAEIDNKPYFVGKDVAKSLGYKNTNDAI
                     LRHCKGVVKHEGFKINGIKIALITEGDVYRLIVGSNLPNAEKFESWVFDEVLPSIRET
                     GQYQAQQHVITELTGTIGDLKGTIKEYKKLCKITCSKKQQYSKYIKNRLDIDKANKEY
                     NQVKARLFLILGVEKWEDIDFDTSNNLIQIIDDCIRVIKSERPYKQLSYFEN"
     gene            13181..13351
                     /locus_tag="PC545_002619"
     CDS             13181..13351
                     /locus_tag="PC545_002619"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457383.1"
                     /translation="MKNNISDSRVKYFCKCPYCGFDNEVEVKKGLKPKICCICTKEVE
                     YEKLEQQSTPAK"
     gene            complement(13354..13587)
                     /locus_tag="PC545_002620"
     CDS             complement(13354..13587)
                     /locus_tag="PC545_002620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457384.1"
                     /translation="MNIGKITKIEATCFDCGTKVTITESKFREVCDNGLPCPMCKEHI
                     SNSNFIAEQALQYNHAVNKLEKELDSCKNIHIY"
     gene            13656..13853
                     /locus_tag="PC545_002621"
     CDS             13656..13853
                     /locus_tag="PC545_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457385.1"
                     /translation="MAINDNINKILRDRNLKAWKLAKEIGVDSGNLYAILRGENKNPT
                     IDTLIKLADYLDVTLDELVGR"
     gene            13892..14101
                     /locus_tag="PC545_002622"
     CDS             13892..14101
                     /locus_tag="PC545_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457386.1"
                     /translation="MAKAVAKEQLFYRAKDIAKILDVCEATAYKIIGELNKELEKEGF
                     KTFSGRVSVAYFKERYCYKPRKGVI"
     gene            14259..14471
                     /locus_tag="PC545_002623"
     CDS             14259..14471
                     /locus_tag="PC545_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457387.1"
                     /translation="MVFNLEKFKVGNAIRISCERFGFEIDCIVVVATEKELNLAYFDE
                     GRGCMEYQALITEDIQDGDYEIKILS"
     gene            14570..14812
                     /locus_tag="PC545_002624"
     CDS             14570..14812
                     /locus_tag="PC545_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457388.1"
                     /translation="MEAARLIAMGQIKQAEKEISKLQGTKNNSSLMWWEAVKFASQNI
                     LQGLEHDIELEASTDFREFMITQEELERDRPIDVQI"
     gene            14896..15405
                     /locus_tag="PC545_002625"
     CDS             14896..15405
                     /locus_tag="PC545_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HDF2457389.1"
                     /translation="MSTLYELTTDLLEIEEGLTETTGNEAEKLEEIKEIIKQEIQNKN
                     TRIVSVILNIDSDINSIDSEIKRLQELKRVKKNTLDRLKSNIKDCMELLGTKKVETVL
                     GNISIRKSAGSLVIEDEEKIPAIYKTVEQVVKVDKNTIKDFIKKGHEVEGCRIEYGTT
                     LTIPKAKKE"
     gene            15415..16029
                     /locus_tag="PC545_002626"
     CDS             15415..16029
                     /locus_tag="PC545_002626"
                     /inference="COORDINATES: protein motif:HMM:NF016302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDF2457390.1"
                     /translation="MEINNIYIKLMDVRVKFSKLNLKKSGENKFANFKYFELADFLPQ
                     ATGLLEEAKLCPIVTFTNEYATLTLINGENPSEQIVFTSPMRDLQLKGSNELQALGGI
                     ETYQTRYLYIQLLNITENDSFDAVSGKEESKSNSNNNVKQSNRILTDKQLTRLYAIAN
                     SVGVNKALLKEQVFKQLGKDIKDLNKSEYDAVCAAYEKQAFKGA"
     gene            16806..16904
                     /locus_tag="PC545_002627"
                     /pseudo
     CDS             16806..16904
                     /locus_tag="PC545_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            16974..17225
                     /locus_tag="PC545_002628"
     CDS             16974..17225
                     /locus_tag="PC545_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457391.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIGFNQVYAILENDIRKIGEYESIER
                     AMQVLNDIQKFIENGVRTDYIDSCRVRHNQEKVFEMPFE"
     gene            17243..17599
                     /locus_tag="PC545_002629"
     CDS             17243..17599
                     /locus_tag="PC545_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457392.1"
                     /translation="MAKVWVDAGTFLERTKDIEDMFEFNLRKVRDRNKKINVIDLRVN
                     NEGQGRKGKKVECFNIVTGETKVFNTAVEASKYVYFTDVYIAHLARTGKVSKNGWKAR
                     YIQEVTDGIGKCGTSN"
     gene            17704..18153
                     /locus_tag="PC545_002630"
     CDS             17704..18153
                     /locus_tag="PC545_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457393.1"
                     /translation="MSLIKYRGYDFENEKWIYSATIMWSNILECLVMLTEGCKWQKVS
                     NVGVCSGEWARNNQEICEGDILKGYDNFHDTSQYGVVKRDIGSIKLYLEWHYLKKLEG
                     EWIELINKTEIYHSRDYKIVGNEYENLEEVRTEFLERKESLENEYLS"
     gene            18256..18432
                     /locus_tag="PC545_002631"
     CDS             18256..18432
                     /locus_tag="PC545_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457394.1"
                     /translation="MDDRLEMINASVNYIQMICESSNIAIIAERGRVRILDLETKEKY
                     DLLKNKLEEMLEEI"
BASE COUNT         7455 a         1893 c         3463 g         5780 t
ORIGIN      
        1 tagaaattca ttttttattt aaggtaaaaa tttaaagtaa aatattattt tacataataa
       61 taaccttata aattttgaaa ttacttatct ttgtgttaaa ataagtaaaa tgcacatatt
      121 agagctaagt ttttaagtaa ataaacaaaa tgaaaatata aatattaagg agatttggta
      181 tatgaatgtt tatgaaaaag gtaatggagt tatactagaa ggagtaacag attttgaccc
      241 agtacatata tttgagtgtg gtcaatgttt tagatggcac aaacaagaag atggctcata
      301 cactggagtt gcaaagggga gaatattaaa tgtcaaaaaa gagaatgata aaatatattt
      361 aaataatact aatttaaagg agtttaacag catttggtat aattactttg atttaggaac
      421 tgattataca gaaataaaga ataagctaaa aaatatggat gaatatttaa ataaggctac
      481 tgaatttgga tggggaataa gaatattaag acaagatgga tgggaaatgc ttatatcatt
      541 tataatttcc tctaataata ggattcctat gatacaaaga gctatagaaa atttatcaag
      601 aaaatttggt aagtatatag gtgaatatga aggtaatgaa tactatgcat tcccaacacc
      661 agaagaacta aataaggcct cacaagagga aataagagca tgtcaaacag gttttagaga
      721 taagtacata aaaagtacta cacaggcagt tatagagaat aatgatgagg tatctgaata
      781 tacaaattta agtactgagg attgtagaaa agagctttta aaatttaatg gagtaggacc
      841 aaaagtatgt gattgtattg cattatttgg tatgcaaaaa tatgattctt ttccagttga
      901 tgtttgggtt aagagagtta tgcaagaatt ttatatagat gaagacatga gtttacctaa
      961 aatgagaact tatggaatcg ataaatttaa agaaatgtct ggatttgctc aacagtactt
     1021 attttactat gcaagagaac tcggtatagg aaaataaatt aagggggaga aattatggga
     1081 gtaataggaa caatatttct gttctattta attatatcat atctagctgg agccataatt
     1141 tctgtgatta tattgcttga aaatagagac cctgcaaaaa caatgtcttg gctattgatg
     1201 tttattatat ttccaggtgt aggtttaatg atatatgcta tttcaggtag aaatattaga
     1261 aaaagaaaat tatttaagac tcaaaaacta gctaataata taaaagaaaa gaaactattt
     1321 gatacattag aaaagataac agaaatagtt gaattagaaa aagaatctat aaaacaaaat
     1381 aagcttttaa gagatgaaga ggatggtagt tatagaaaaa gggtaataaa tatgttgtta
     1441 aagactggta tgtttccctt tactaaaaat aataaagtgg atgtatttgt agatggaaat
     1501 gaaaagttta aaagacttat agaagatata agagaagcca aagaccatat tcatttagag
     1561 tactttatca tcaaagatag tgaaataggt agggtgttaa aagaagaact tataaaaaag
     1621 gctaaagaag gcattaagat aagaatttta tatgatgatg taggatgctg gagattttgg
     1681 tttaatagaa aatttttccg agaaatgagg gaagtaggta tagaaattgc agcattttta
     1741 cctacaaaat ttcctataat aggagggaaa ttaaactata gaaatcacag aaaaattgtt
     1801 gttattgatg gaataattgg atatacaggt ggaataaata taggagatga atatttaggc
     1861 aaaaatgata agtttggata ttggagagat actcatatta ggattaaggg tatttctgta
     1921 tacatgcttc aaatgacatt tctgattgat tggtattata cgacaaaaga agtattagtg
     1981 actaaaaatt atttcccaag tgtagggaat gttggtgaaa gtatgataca agttgttgcg
     2041 agtggtccag acagtgactg ggaagatatt cattatgctt atttttctgc aatatgtcag
     2101 gctagaaaaa atgtttatat agaaactcca tattttattc cagatgaaag tctactaaaa
     2161 gctataaaga gtgctgctct tagtggagtt gatgtgagaa taatatttcc taaaattgca
     2221 gaccacaaga tagtaaatat agcatcttat tcatattttg aagaaatatt aagagcaggt
     2281 ggtaaagttt atttatataa taaaggattt atacattcta aagtcgtaat aatagatgat
     2341 aaaattgctt ctgcaggtac agctaatatg gatttaagaa gttttatgct taactttgag
     2401 gtaaatgcat ttatctatga tgaagaagtt attagggtaa tgacagatga tttctttgag
     2461 gatttaagtc actgtgaaga acttaatttg gaggtattta agaatagaaa tatcatacaa
     2521 aaaatcaaag aatctgtagc aagattattt tcaccaatat tataaaaaat tattaaaaaa
     2581 ggtattgaaa attatgtgtg attggtatat tataataatt gttagcactc tattagatag
     2641 agtgataata aataaaaagt atatatataa atctacatat ataagtagta ttaatttaaa
     2701 aataactaaa aacataggcg aactagtcta tacttaatat tttaggaggc gtgaataatg
     2761 aaaataagac cattagctga cagagtagta attaaaaaag tagaagcaga agaaaaaact
     2821 gcaagtggaa tagttttacc aggagcagct aaagagcaac ctcaaatagc tgaagttgta
     2881 gaagttggac caggtggaat agttgaaggg aaagaaataa aaatggaatt gacagtggga
     2941 gataaggtta tattccaaaa atattctgga acagaagtta agatagaagg acaagaatac
     3001 acaatactaa gacagagtga tgtattagct gttattgaat aaatatagaa taaatttatt
     3061 aggaggggtt taaaatggct aaagaaatta aattttcaga ggaaacaaga agagctttag
     3121 aagctggtgt aaataaatta gcagatacag taaaagtaac attaggacct aaaggaagaa
     3181 atgttatatt agataaaaaa tttggttctc cacttataac taatgatgga gtaactatag
     3241 caaaagagat agaactagaa gatagatttg aaaatatggg tgctcaatta gttaaagaag
     3301 tagcaactaa aactaatgat gtagctggag atggtactac aactgctaca gttttagcac
     3361 aagctataat aagagaaggt ttaaagaatg taacagcagg ggctaaccca atacttttaa
     3421 gaaaaggaat acaaaaagca gtaacagtag cagtagaaga attaaaaaat caatcaagaa
     3481 tagtagaaac acaagaggct atatctcaag ttgcttctat atctgctggt gatgaagaag
     3541 ttggaaaatt aatagcagaa gctatggaaa tagtaggtaa agatggagtt ataactgttg
     3601 aagagtctca aactatgaat actgaattag atgctgttga aggtatgcag tttgatagag
     3661 gatttgtttc tgcatatatg gttacagatg tagataaaat ggaagcagtt ttaaatgacc
     3721 catatatatt aattactgat aaaaaaatat ctaacataca agaattatta ccagttcttg
     3781 aacaaatagt tcaacaaggt aaaaagttat tgataatagc tgaagatgta gaaggtgaag
     3841 cattatctac attagtagta aataaattaa gaggaacatt tgatgtagta gcagttaaag
     3901 ctccaggctt tggagataga agaaaagaaa tgcttcaaga tatagcaata cttacaggtg
     3961 ctcaagtaat atcagaagaa ttaggttatg atttaaaaga agctgattta tctatgctag
     4021 gtagagcttc atctgttaag gtaactaaag aaagtactac aatagtagat ggttctggag
     4081 ataaaaaagc tatagaagat agagtaactc aaattaaaca tcaagtagag caaactactt
     4141 cagattttga tagagagaaa ttaatggaaa gattagctaa acttgctgga ggagtagctg
     4201 ttgtaaaagt tggagctgct acagaagtag agttaaaaga aagaaagtta agaatagaag
     4261 atgctcttaa tgcaactaga gcagctgtag aagaaggaat agtagctggt ggaggaactg
     4321 cttttgttag tgttatacca gcaataggaa cactaattga gagtttagaa ggagaagtta
     4381 aactaggtgc tcaaatagtt aaaaaagcgt tagaagagcc attaagacaa atagctataa
     4441 atgctggtct tgaaggtgct gtaattgtgc aaaatgttgt taattctgaa gcagaaactg
     4501 gatttgacgc attgaatgaa aaatatgtaa atatgataga agctggtata gttgacccaa
     4561 ctaaagttag tagaagtgct ttacaaaatg ctgcatcaat agcaagtact ttcttaacta
     4621 ctgaagctgc tgttgctgac cttcctgaaa aagaagatgt aggaatgcca ggaatgggtg
     4681 gcggaatgcc tggtatgatg taatttaaaa aatttaaaat ataaatttta atataaagaa
     4741 ctatttcgac ttagatttta aactctaagt tgaaatagtt cttttgtata tatctaattt
     4801 ttgtatatct ataaattcaa tacacatatt tagataatca gtatctagtt tgtaataata
     4861 ttttagagta gtattgttat tttttaggtt atgattttag atatatatta attaaggtat
     4921 aattgttgat agagaataaa tcaaatttaa aaggatgcga taatatggac ttaagaaaat
     4981 tgcttgaaga agttaaaaat gaaaatattg atatagattt tgcgttagaa aagttaaaag
     5041 atttacctta tgaagacctt ggatatgcaa atatagacca tcatagagaa ttaagaaatg
     5101 ggtatccgga agttatttat tgtgaaggta agagtgatga gcatatttta gggattattg
     5161 ataaaatgaa tcaaaaaagt tctaacatat tggggacaag gtgcagaaaa gaaacttttc
     5221 tgaaaattaa agaaatctat aatcatgctg agtacgaaga ggcgtctaaa attcttaaga
     5281 ttcaaaatca tgatatagag aatataggaa aaggtaaaat tgtgattgct acagggggaa
     5341 cttctgatat accagtagca gacgaagctt atcatacagc taagttttta ggtaatgatg
     5401 ttgatagaat atatgatgtt ggagtggcag gtatacatag gcttttaaat aaaagacata
     5461 aaatagatag tgctagagta atagtagccg ttgcaggtat ggaaggtgct cttgcaagtg
     5521 taattggtgg actggttgat gttccagtaa tagctgttcc aacttctgtt ggatatggag
     5581 caaattttgg aggattagca gcattactag ctatgttaaa tagctgtgca tctggaatat
     5641 ctgttgtaaa tattgataat ggatttggag caggatattt agcatcaatg ataaataaac
     5701 tgtaaataca ttgatataaa ttaaacaaaa ctatataata aaatttaatt tctattatat
     5761 ttttactatt aaaattgagg agacatatat gagtgaaaaa atactttatt ttgatataat
     5821 aagcggtata tcaggggata tgacccttgc aagtttatta aatttaggtg tgcctaagga
     5881 aatttttttg gaggaaattc ataaactaag aatgagcgat gaatttaata taaacatcag
     5941 ctcgaaaact gaaaatggaa tagtaggtac aaaagtagaa gtaattacaa aagaaaaaca
     6001 tacacataga aatttagtgg atatatttga ggttatagat aaaagcactc taaacgaaaa
     6061 tgtaaaatct aaagcaaaaa aaatatttat ggtaattgga gaagcagaag caaaagttca
     6121 tggaactaca atagacaaga tacattttca tgaggtaggg gctatagatt caattgtaga
     6181 tatagtagga gcttctatat tagttgactt actttgtgta gataaagttt gtgctagtac
     6241 agtgtctgtt ggttcaggat ttgtaaaatg tgaacatgga attataccta tacctgcacc
     6301 tgcaactata gaaattttaa aaggtgtgcc aattaaatta aataatgtaa atggagaatg
     6361 tacaactcca acaggagctg caatcataaa agtcttgtgt gatgagtttg tagatgagct
     6421 tgaattttct ccaaagcaaa ttggatatgg tataggtcat aagaagtttg aagtaccaaa
     6481 tatactcaga actgtattag gtgaaaaaaa aaagaagaat taatttatga aattagtgca
     6541 aacattgatg atatgtcttc agaaatctat tcttatttgt atgaaaaaat attagatgag
     6601 aaagcattag atatttatac agagagcatt tatatgaaga agaatagacc agcaacaaaa
     6661 gtatcaattt tatgtaatga atcagattta gaaaaattta ttaaaatatt acttttagaa
     6721 actagtactt ttggagtgag atataatgca tataatagag aaattctaga gagaaaattt
     6781 gtaaaattag atacaaaata tggagtggtt acagtaaaac tagggtatta taaaggaaaa
     6841 ctagtcaagg ctactccaga atataatgaa tgtaaaacta tagcaaaaaa tgaagatatt
     6901 cctataaaaa aagtgtttgc tgatataaat tttagaataa aagaagaatt tagtgaaaac
     6961 ttattgacat aagtaataaa ttttgataaa ttaatattca gccttattaa ataaaagtaa
     7021 acagaaaaaa atatataaaa tataaactca ctcgtatatg ctcagaaata tggtctgaga
     7081 gtctctacca agataccgta aatatcttga ctatgagtga aattattata ccaaaagacc
     7141 tagttaagta aatgtgtagg ctgagttaaa ctccctatgt gttggctata agataaaggt
     7201 attttggaag cctgtatagt aatttcactt ttgctagaaa ttatggaagt tactatacag
     7261 gttttttgtg taaaaagatt taatataatt tagactgtat tgtgttaaaa taaatatata
     7321 aaaactatat tatacaagga gagatgtaaa tttatgaaac atgaattagt acttgtaata
     7381 gattttggag gacaatataa tcagttgata gctagaagag ttagagaaaa caacgtatat
     7441 tgtgaaatac ttccatgtac tgcaagcata gaaagaataa aagaaaaaaa tcctaaaggg
     7501 ataatattta caggcggtcc aaatagtgcg tatttagaag attctccaac aatatcaaaa
     7561 gaaatatttg aactaggagt accaatactt ggaatatgct atggtattca aataatgtct
     7621 catgtattgg gtggagttgt aagaaaaggt aataaacaag aaaaagaata tggaaaaaca
     7681 gctataactt atggaaaatc atcattattt gaaggaataa ctacaaatag tgtatggatg
     7741 agtcatacag atttaataga aaaagttcca gaaggattta ctatagttgc aaatactaat
     7801 gattgtccag tagcagctat ggaaaatgta gaacgaaacc tatatggagt tcaattccat
     7861 ccagaggtag aacactgttt ggaaggggac aagatactta caaacttctt atataatatt
     7921 tgtaaagtaa aaggtgactg gactacagat tcattcatag aagataaaat aaaagaatta
     7981 aaagaaaaaa taggcgataa aaaagcttta tgtgctttaa gtggaggagt tgattcttct
     8041 gttgctgcag ttcttattca taaagcaatt ggggacaact taacttgtat atttgttgac
     8101 catggtttac ttagaaaaaa tgaaggaaat gatgtagaaa gaatatttag agagaaattt
     8161 gatataaatt taataagagt taatgcagaa gatagattct tatctaaatt aaaaggagtt
     8221 tctgaaccag aagctaagag aaaaataata ggtgaagaat ttataagagt atttgaagaa
     8281 gaatcaaata aattaggaaa aatggatttc ttagtacaag gaacaatcta tccagatgtt
     8341 atagaaagtg gacatggaaa tgctgctact ataaagtctc atcataatgt tggtggaatc
     8401 ccagaagatg tagacttcca agaaattgta gaaccattaa gagagttatt taaggatgaa
     8461 gttagaaaaa taggattaga gcttggtata gaagaaggat taatatttag acatcctttc
     8521 ccaggaccag gtctaggaat aagagttata ggtgatgtaa ctaaagaaaa atgtgatata
     8581 ttaagagaag ctgatgctgt ttatatggac gagcttagaa aagcaggttt atacagagaa
     8641 atatggcaag cttttgcaac attaccagat gttaagactg ttggagttat gggtgatgaa
     8701 agaacatatg cttatttagt tggtttaaga gcagttactt cttctgatgg aatgacttct
     8761 gactggtata agatgcctta tgatgtatta gaaagaattt ctaatagaat aataaatgaa
     8821 gttgatggag taaatagagt agtttatgat ataacttcta aacctcctgg aactattgaa
     8881 tgggaatagt aaataaaaaa gtcgtaagcc ttgtattttc aaggtttgcg acttttaaaa
     8941 aaatctttgt gatactaaaa tgatactata tatttaaatt atctagtttt tgggcaactt
     9001 caagttgctt atttgggtat aaatgtgaat atgtattcca agttgtttct actttttcat
     9061 gccccaatct ttctgctata gttaatatat ttacatccat atttaccaat aatgacgcat
     9121 gagaatgtct caaatcatgc actctaattc tttttacatt agataactta cagcatctat
     9181 ctagctcttt agaaagataa cttttagcaa atttaaatat tctttcatta tcttttaagt
     9241 catatagctt agatagataa tcttttatat tattatataa gaaatgtggg atagggacaa
     9301 cacgtttact tttaggagtt ttaggagaag aaacaatatc ctctccattt agtcttatgt
     9361 agcttttttc gacgcttatt ttattttcga aaatatcttt tggagtaaga gctaacaact
     9421 cccctagtct aagacctgtc caaaataaaa tttcaaatgc tagtctaggt tctgatttct
     9481 tttcaaattc aataaatttt ttaaactctt ctaatgtcca aaagttcatt tcatctgcgt
     9541 tttttttacc gatagaacct gccaaatgag caggattaga aggtaagtta taatatttaa
     9601 ctgcataatt taatacagca acaagctggt tgtttatcgt tttaatatat gtttggctat
     9661 aatcactttt taataattcg ttttgccatc ttcttatatg tgttgcagta atttcattta
     9721 tttttagact tttaaaaaaa ggtaaaattc taaagtttat taaatacttt ttagtttcta
     9781 atgtagataa ttttaatcta gaagacatat cgtgcatgta ttcttctatg agactttcaa
     9841 aactcatatc agtactcatt ttagatttat tcaaaaattc tctttcaaac tctagagctt
     9901 cttttttagt tttaaaacct ctcttaattt tctttttcct atccccatcg aagtctgtaa
     9961 aataaaagct agcataccaa ctttttcttt gttcgtcttt gtaagcaggc attttagaca
    10021 cccccaatta tgtcatttta atattacaat aattataaca tgttagatat gctatctaaa
    10081 taattgtttt attttcctct tctttttatt tcttgaagct tgtaatattt acacagttct
    10141 aagaagtctt cttctagtgc tatagataag tctaatatcg tagatatacc tatatctttg
    10201 tactcttgat tttctagttt tgatatataa cttctgttac gtctcatttt ttctgctaat
    10261 tctagttgtg tcattctttt cttttttctt aattttttca acatttatta aaatccacct
    10321 tttaattatt tttatgacat ttttataatt aatagtttgt gtaatttctt gtaaaaaatg
    10381 ttcctcatta gaacattttt tggtggaaaa atgtgttaaa atgtaagtaa gaaaagcact
    10441 ttcaaagcac tataaaaata gtgctgaaat agataaacaa ataaaagtaa taaggaggaa
    10501 agatagattc tagagggaaa tcattatcat ttttaagaac gtatgttcgg ttggtgggat
    10561 agaaaaatac tttacagggg atggtttatt tgaatgagaa tgaaaatatg agtttatatg
    10621 tcagtaagtt aaaagaagta ttgaaaaata attcagaaga gtataaaaaa ataagagcta
    10681 aaataaatga aatttataat ctaaatgaaa agaaagaata aaagggcttt agctctttta
    10741 ttctttctta gtattaactg ctttataata agcattatct atcatttttt ttattgcatt
    10801 tttatcatct gaatctaaag atgctaatct atttattagt tcgttaactt cattatcttc
    10861 ttttgagtta acttttttta ataattcatc atcttgttga gtgttaggtt gtattttacc
    10921 taatttagtt aatagctcct ttaaagttaa atttaatcca aaactcattt tttcaagcat
    10981 atctaaagta ggttcaacag ctttaccatt ccttgggtct ctgttttttt ctatcttatc
    11041 tatatatgta tggctcacac cacaaagatt agaaaattct ctaagagata agttatgttc
    11101 acttctatat tctttaataa tttctgctaa tgtttgcata atttcacacc tcatctaagt
    11161 aatagttgac ttaattatat cagaaaaatg taaactattg ttaacaaaaa taaaaattta
    11221 gtaaaaaata attgacaaga gtaaaaaaaa gacgtatact atgattaaca aaaaggttat
    11281 aaataaagtt aggagggatt aatataaaaa ataatttaca atatattaga aaagaatcta
    11341 gaatatcaca aaaaaaattc gctgataaga taggtatatc aagaccgtat ttatctagaa
    11401 tagaaaacgg taaggttaat ccgagtttag aaatagcgca taaaatctcg ctcgaaacag
    11461 ggaaaacaat aaatgaaatt ttttttgatt ttactgtaaa tcatagtaaa ctataaggtt
    11521 ttaattgtac cttgaaaact aaatataaaa tattttaaag gagcaagtat atgaaaaata
    11581 aagaaaaaga aataatcatt agaggaattt taataggaat tttttatttt ttaggaattg
    11641 tatttagtaa tacttttttt aaataataaa tagaaggggt aggtgaaata ccaactcccc
    11701 aaatcaaaat aacactttgg aagttaaata taaaatattc aaaagaggtg attagatgca
    11761 aagagagcaa acaacgcttc gtataccaga agacttgcac aaagcgttga tagatttaag
    11821 cagtgatata ggaatgccta ttacatctat tataataatt gcatgttggt tgtatatatc
    11881 aaagataaat taaccaatcc gatatgcaaa atgaatgcac aacattattt attagagaat
    11941 tgattgataa attattttct ttagctgttt tctctagcaa ctcttttagg acaccaggaa
    12001 tcctaagagt agaacgaaca gtatcatcac ttttatacga gattgattga agttcattaa
    12061 cttttgaatt tctaataata tcattaatag cgtatagaat aagcgaagat ttgagaatgc
    12121 ctgtttgata agaaattttt tctaaatttt catttaatgg tgtatatatt cttacagtaa
    12181 tagtagtcat tttgacacct ccctaatgac attatacaaa aaatatttaa aaaagtcttg
    12241 acactaaaat agtgtcataa tataatgatt atagacagta aaacagtgtc ataaggaggg
    12301 aggaataaga atggaaaaag taagaatgac agtaaggctc gtcccaaaat taaatcaata
    12361 tgtacagaat atagccaaag aatgtggaaa aagtaaaaat tctattattg tggatgcatg
    12421 ttgggagttt attgagaaaa taaaaaaaga aaataaattc aatgtagaaa gcgaggaata
    12481 aatatgaata atctacagat atttgaaaaa ttggaatttg gacaaataag aatggcagag
    12541 attgataata agccttactt tgtaggaaaa gatgttgcaa agtcattagg ttataaaaat
    12601 actaatgatg caattttaag acattgcaaa ggggtagtga aacacgaggg gtttaaaatt
    12661 aatggtatta agattgcttt gataactgag ggtgatgttt atagattaat agtaggttca
    12721 aatctaccaa atgcagaaaa gtttgagagt tgggtgtttg atgaagtgct tccaagcata
    12781 agagaaacag gtcaatacca agcacaacag catgtaataa cagaacttac aggaactata
    12841 ggagacttaa aaggaacaat aaaagaatat aaaaagttgt gtaagataac atgctctaag
    12901 aaacaacagt attctaaata cataaagaat agactggata tagacaaagc taataaagaa
    12961 tacaaccaag tgaaagcaag gctattccta attcttggag ttgagaagtg ggaagacata
    13021 gacttcgata catctaataa tttaatacaa attatagatg attgtataag agttataaag
    13081 tcagagagac catacaagca gttgagttat tttgaaaact aaatacagaa tattttgaaa
    13141 tatattgttt taattaatta ttaactagga ggttaaatac atgaaaaata atataagcga
    13201 ttcaagagta aaatattttt gcaagtgtcc atactgtggg tttgataatg aggtagaagt
    13261 taaaaaagga ttgaagccta aaatatgttg catatgtaca aaagaagttg agtatgaaaa
    13321 actggagcaa caaagtactc cagcaaaata aaattaataa atatgaatat ttttacaaga
    13381 atctaattct ttttctaact tattaacagc atggttatat tgcagtgcct gttctgctat
    13441 aaagttagaa tttgatatgt gttctttaca catagggcat ggtagcccat tatcacaaac
    13501 ttccctaaat ttagattctg ttatggtaac ttttgtacca caatcgaaac aagtagcttc
    13561 aattttagtt attttaccga tattcataaa tcaccaccta atcatatatt tataggattt
    13621 atcctacaaa tatagtatat caaaggaggg aaataatggc aattaatgac aacataaata
    13681 aaattttaag agatagaaat ttaaaagcgt ggaaattagc aaaagaaata ggcgtagatt
    13741 caggaaattt atatgcaatt ttaagaggag aaaataaaaa tccaactata gatacattga
    13801 taaaactagc tgactattta gatgttacat tagacgaatt agttggaaga tagaaattaa
    13861 atacagaata ttttgaaagt aggtgtattg tatggcaaaa gcagtagcta aagaacaatt
    13921 gttttatagg gcaaaagata tagctaagat tttagatgta tgtgaagcaa ccgcttataa
    13981 aataattgga gaattaaata aagaattaga aaaagaaggt tttaagactt tttctggcag
    14041 ggtatcagtt gcttatttta aagaaagata ttgttataag ccaagaaagg gggtgattta
    14101 gttgaatgta aaaggtacta atagcttata tacagttttg caatgataag caaataaaag
    14161 caagttttga aggtcttaga aaatacaaca gagatataga gttagttaac ttgtatttac
    14221 caagtaaatt aaaaataaat aaggaggtct aattgtatat ggtatttaat ttagagaagt
    14281 tcaaagtggg aaatgcgata agaataagtt gtgaaagatt tggttttgaa attgattgta
    14341 ttgtagtagt agcaacagaa aaagaattaa atttagcata ctttgatgaa ggtagaggct
    14401 gtatggagta tcaagcctta ataacagaag atattcaaga tggtgattat gagattaaaa
    14461 ttttatctta ggaggaaata aaatggcagc tttaataatg gtaggtttat ttgcaatatg
    14521 tttagtagga ttagtacaaa atagagatta agtgaagggg tgtatttaga tggaagcagc
    14581 tagattaata gcaatgggtc aaattaaaca ggctgaaaaa gaaataagta aattgcaagg
    14641 tacaaaaaac aatagtagtt taatgtggtg ggaagccgta aaatttgcta gtcaaaatat
    14701 attacaaggg ttggaacatg atatagagct agaagcatca actgatttca gggagtttat
    14761 gataacacaa gaagaacttg aaagagatag acctatagat gtgcagatat aagaaaagag
    14821 cctagggtga ggctcaattc aaataaatat taaaaaattt aattaagcta attatagcat
    14881 aaacggaggg aaattatgag tactttatat gaattaacta cagatttatt agaaatagaa
    14941 gaaggtttaa cagaaacaac aggaaatgaa gctgaaaaac tagaggaaat aaaagaaata
    15001 ataaaacaag agatacaaaa taaaaacact aggatagttt cagtaatatt aaacattgac
    15061 agtgatataa actctataga ttcagagatt aaaagattgc aagagttaaa aagggtcaaa
    15121 aagaatactc ttgatagatt aaaaagcaat ataaaagact gtatggaatt acttggtact
    15181 aaaaaagtag aaacagtttt aggaaatata agtataagaa agtcagcagg tagcttagtc
    15241 atagaagatg aagaaaagat acctgctata tataaaacag tagagcaagt tgtaaaagta
    15301 gataagaata ccattaaaga ttttattaaa aaaggtcatg aggttgaagg ttgcaggatt
    15361 gaatatggaa ctacactaac aattccaaaa gctaaaaaag agtaggtgag gaccatggaa
    15421 attaataata tttacattaa attgatggat gtaagagtta aatttagtaa gttgaatcta
    15481 aagaaaagtg gagaaaataa gttcgctaac ttcaagtatt ttgagttagc agactttcta
    15541 ccacaagcaa ctggattact tgaagaagct aagctatgcc ctatagtgac ctttacaaat
    15601 gaatatgcaa ctctaacatt aattaatgga gaaaacccat cagaacagat tgtatttact
    15661 tctcccatga gagatttaca acttaaaggt tctaatgaat tacaggcact aggaggtata
    15721 gaaacctatc aaactagata tttgtatatt cagttactaa atataactga aaatgatagt
    15781 tttgacgcag taagtggaaa agaggaatct aaaagcaatt ctaataacaa tgttaaacaa
    15841 agtaacagaa tattgactga taaacaacta actagattat atgcaatagc taatagtgtt
    15901 ggagttaata aagcattatt aaaagaacag gtttttaaac agctaggaaa agatataaag
    15961 gatttaaata agagtgaata tgatgctgtt tgtgcagcat atgagaaaca agcctttaag
    16021 ggtgcataac aggaggtgat agagtggcag ttttcagaca gatatataca agcttttgga
    16081 ctgacccaaa ggtacaagag gagtggacac cagaagataa atttttcttt atacttcttc
    16141 ttagtaatcc tcaaacaaca caaataggag tttaccaagt aactaaaaaa caactagcat
    16201 tttggatggg gtactcagaa gaaagtataa gagcattaat ggatagattc gtaaatcacc
    16261 ataagtgtat taaatataat cccgatacta gagaaattgc cataaaaaac tggggcaaat
    16321 ataacttaac taaaggtggt aaaccaatta tagatttact taataaagaa ctcaaagaag
    16381 ttaaagatat tgagttaata aaatatgtaa ttccatcaat agaaaaatca gatataagaa
    16441 aaatatttga ggattattac gaaatggcta aaaatgggga ttcttacgat acgtcgacga
    16501 tacgtgaacg agtcgtacca cgatacgggg acaataacaa taagaataac aataagaata
    16561 acaataagaa taacaacaac aataaggatg attcggtggt tgtggataaa ataaaacaat
    16621 actttgattt agaatctaaa gacattgaaa aaattgttga tatattcata catacaggaa
    16681 gaggaattga ctatttagag gaaaaactaa ggttggtaaa aaatacagat ggagtaaaaa
    16741 gtattacagg ctatctcata aaagcattag aagaagatta taagcctata ccaagtagaa
    16801 aaaataagac taagtttcat aattttaacg agacatttac taagtatacg tctgatgaac
    16861 tagacgagat tattaaaaag agtcaaaaag agaagttcag ttgaattaaa tagtctaggg
    16921 agtagttata caatattact tcctaggagt taaaaaatat tggagggata gaaatgataa
    16981 taattagaag tcaagataga ttagatttaa tgagagttaa tagagttgaa ataggtttta
    17041 atcaagtgta tgcaatactt gaaaatgata ttagaaaaat aggtgaatat gaaagtattg
    17101 aaagagctat gcaagtgtta aatgatatac agaagtttat tgaaaatgga gtaagaacag
    17161 attatataga ttcttgtaga gttagacata atcaagagaa agtatttgaa atgccatttg
    17221 aataaaaagg ggggaactaa atatggctaa ggtttgggtg gatgcaggaa catttttaga
    17281 aagaactaag gatatagaag atatgtttga atttaatcta agaaaagtaa gagatagaaa
    17341 taaaaaaata aatgtaattg atttgcgtgt aaataatgag gggcaaggaa gaaaaggtaa
    17401 aaaagtagag tgttttaata ttgtgactgg agaaactaaa gtatttaata ctgctgttga
    17461 agcaagtaaa tacgtatatt ttacagatgt ttatattgct catttagcaa gaacaggtaa
    17521 ggtttctaaa aatggatgga aagctagata tattcaagag gtgacagatg gtattggcaa
    17581 atgtggaaca agtaattaag ttagctgaaa agatattaaa taagaaaaag tgttctgtta
    17641 ataaagctat tgatatagtt ataaaaatat taagtaaata tgagtgcgag gggatgttaa
    17701 gaaatgagtt taattaagta tagaggttat gattttgaga acgaaaagtg gatttattca
    17761 gcaacaataa tgtggagcaa tatacttgaa tgtttagtta tgttgacaga gggttgcaaa
    17821 tggcagaaag tctctaatgt tggggtatgc tctggagaat gggctagaaa caatcaagaa
    17881 atttgtgagg gggatatatt gaaaggatat gataatttcc atgacacaag ccaatatgga
    17941 gttgtaaaaa gggatattgg tagtattaag ttatatttgg aatggcatta tttaaagaaa
    18001 cttgaaggag aatggataga gcttataaat aaaacagaaa tatatcatag cagagattac
    18061 aaaatagttg gtaatgaata tgagaactta gaggaagtca gaacagagtt cttagaacgt
    18121 aaggagagtc ttgagaatga atatcttagc tagtgcgata ttagtaatag gaagttttat
    18181 agctggtaga gtttatgagt atagattgaa tctaaaagag tgtgaaaatt gcgacaataa
    18241 aagaggtgta taagaatgga tgatagatta gaaatgataa atgcttctgt aaattatata
    18301 cagatgatat gtgaaagttc aaatatagct attatagcag agcgaggaag agttagaata
    18361 ttagatttag aaactaaaga aaaatatgat ttattaaaaa ataaactcga ggaaatgtta
    18421 gaagaaatat aagtgaaaat atctaattaa gacagtttag agagttacaa aatatctttt
    18481 agtataaatt attgttgaag tgttttgtga ctctcaaaaa tgaaaataag gaggcgttgt
    18541 attgcttaca tttttagatt tattcgcagg gataggtggc tttaggctag g
//
LOCUS       DAMJVI010000077        18534 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.079, whole genome shotgun sequence.
ACCESSION   DAMJVI010000077 DAMJVI010000000
VERSION     DAMJVI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.079"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(212..892)
                     /locus_tag="PC545_002632"
     CDS             complement(212..892)
                     /locus_tag="PC545_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457395.1"
                     /translation="MQTKILIIDGDKDNCQKLKGFLEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILSDGDGWTLCKKIRNVTTCPIVYMTYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(942..2957)
                     /locus_tag="PC545_002633"
     CDS             complement(942..2957)
                     /locus_tag="PC545_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2457396.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTYKSIRASNG
                     ILTIDNDTFTKGKLVFLIDDWEFYNHKIFKPNDFTEQDIKPEYVFIGQYPDFSMRKGT
                     QSPYGNATYRMRIKNEGKDKLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NSVVSFIAKKNTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRLLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKIYLYFGCMCVFFAIHVSYPFVHLLGLPLVSFTYAVEDLSY
                     FMVVLCIICINGSISKIEENKVYRFLILPLGITMSIISIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAVTSNRFEP
                     IFTGWQTEYCGFVIVILFSVMMINRNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KRFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNNFIKEIYENNLPDTQACGVNFETYVLEKKIYAKINKEMLT
                     RAFENLIYNALTFTPFDGKIILSMDKEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNEPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(3029..3706)
                     /locus_tag="PC545_002634"
     CDS             complement(3029..3706)
                     /locus_tag="PC545_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457397.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
     regulatory      complement(4286..4369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(4602..5444)
                     /locus_tag="PC545_002635"
     CDS             complement(4602..5444)
                     /locus_tag="PC545_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2457398.1"
                     /translation="MFKKKIGVLLASTMLVGALAVGCSSNSGGSGDSGDSSKVSVSGS
                     TSIGPLMETIGEKFQEKNQGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEEKSA
                     GLKETKIAIDGIALITNTANSVKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVSREE
                     GSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKVNSL
                     KINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQALVSEKGFI
                     KVNQ"
     gene            complement(5696..7480)
                     /gene="pepF"
                     /locus_tag="PC545_002636"
     CDS             complement(5696..7480)
                     /gene="pepF"
                     /locus_tag="PC545_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDF2457399.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSSKEEIEKDISKVKNLIQEVKEYK
                     GKLSESKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDEKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTIPENVYDMLAYADMEFPEIEDEEGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLVHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVHRQTMFAEFEKIVHQRVESGEPLTADEFTNIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(7546..8211)
                     /locus_tag="PC545_002637"
     CDS             complement(7546..8211)
                     /locus_tag="PC545_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDF2457400.1"
                     /translation="MIDIGSKEIAIRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGIAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKNQLIVRNMEIECRNEEYWKVNFFVSYAKRIHLHDVMKEINMIEGVINVD
                     YLD"
     gene            complement(8362..8523)
                     /locus_tag="PC545_002638"
     CDS             complement(8362..8523)
                     /locus_tag="PC545_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HDF2457401.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(8601..9110)
                     /locus_tag="PC545_002639"
     CDS             complement(8601..9110)
                     /locus_tag="PC545_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDF2457402.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKDL
                     SLIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            9316..9534
                     /locus_tag="PC545_002640"
     CDS             9316..9534
                     /locus_tag="PC545_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2457403.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            9552..11498
                     /gene="feoB"
                     /locus_tag="PC545_002641"
     CDS             9552..11498
                     /gene="feoB"
                     /locus_tag="PC545_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2457404.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLSEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIVLNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFTSFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIMEALIGGIIIVLALFILFNKFKSTKNGNVCSGCS
                     ICSGCPSANSCSSKEDSNKKNNAH"
     gene            complement(11677..12732)
                     /locus_tag="PC545_002642"
     CDS             complement(11677..12732)
                     /locus_tag="PC545_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2457405.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKRTNLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGIISSEKYNEEINKILDAINRFEAVYKLSKQWIERNKEKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGVDPSV
                     PKDTRFHMKLGSKKLNK"
     gene            complement(12780..13580)
                     /locus_tag="PC545_002643"
     CDS             complement(12780..13580)
                     /locus_tag="PC545_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2457406.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLAKEGNIFGPLLFLLLYNIPAFAFRIFGLKYGY
                     QVGVNSLERIQREGLMEKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVTGIALHAIGLL"
     gene            complement(13573..14364)
                     /locus_tag="PC545_002644"
     CDS             complement(13573..14364)
                     /locus_tag="PC545_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2457407.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMLDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGIAGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQKAVFYAKQADFKKVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKVILDGLGVAGTLLPAVGFALLMDMLLSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLTEGEIDFE"
     gene            complement(14404..14826)
                     /locus_tag="PC545_002645"
     CDS             complement(14404..14826)
                     /locus_tag="PC545_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDF2457408.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTTDKSADDA
                     EKEIRSILSNNKDSEEFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIVKSGLEEAKNGIVYVNELLENQNKIKNVM"
     gene            complement(14842..15321)
                     /locus_tag="PC545_002646"
     CDS             complement(14842..15321)
                     /locus_tag="PC545_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2457409.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVVAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDDDDINNFKQIGDLGIDIEFRMLPRDKKKQLSDLIK"
     gene            complement(15453..18209)
                     /locus_tag="PC545_002647"
     CDS             complement(15453..18209)
                     /locus_tag="PC545_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457410.1"
                     /translation="MRDEILSFVNECVLFNSFDELKKESGIITEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKNKINDVVGPTKKSIYENLDELKNECKLLKQETV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YVPNIEERGTIEKRKIVEHFIYEESKVLGLPIEITQITLNAILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIIFSPKQFSIEM
                     SNGRNKRNIIYQSFIKCLDYFDRLEKNEITKEKFSKKSSNLMIRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLITNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNATI
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKNKINSPMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGIAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVMAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSRVVENEKIKNIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLSNDKKISLYVHLSCM
                     IERLVRQTEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQIPISEIGYIYNIIY
                     GLPQ"
BASE COUNT         6079 a         3164 c         2035 g         7256 t
ORIGIN      
        1 acaatttatc catcttttca aatataatct ataaaataat agttccatta cgcatttttt
       61 tatataataa aactgactgt aatttaactg gcaaagtcta gaagaatagg atttatcaat
      121 taaattacag tcagctattt tataataatt tagtttagat atcgttagta tttcacattt
      181 ataacaatta ttaatgatat taatatattt attattcctt attaaaataa tatcctgctc
      241 ctcttattgt tataattttc tgaggatttt tataatcttt ctctattttc tttcttaatc
      301 cattcactgc aactaccaca ctatagttat cctctaagta ttcattcatc catacatgtt
      361 catatatctc tttagtagtc aaagtctttt caggattctc tataaaaaag tttaacaact
      421 tattttctgt tggagtcaat tttattattt tatcttctaa ctttattact ccactagttc
      481 tattatattc ctctaatttt gtattatttt cattattatt tacatatgag ttcattcttc
      541 ttaaaatagc ttttactttt gcgtataata tttctaaatt tagaggcttt attaagtaat
      601 cgtctccacc agaatttaaa gcatttaaaa tgctttggtc ttcatttata taagtcatat
      661 aaacaattgg acaagtagtt acatttctaa tttttttaca aagagtccat ccatcaccat
      721 cacttagaat tatttctaaa aatattaaat catatttatt gctaaatatt tttccaatag
      781 cttcttcaca attatatgct aaatctatac ttattccttt ttcctccaga aatcctttta
      841 gcttctggca attatccttg tctccatcta ttatcaatat cttagtttgc attataatca
      901 ctcccaaata tcattattct tttatattaa ataagctatt attataaaaa acatagactt
      961 aattttattg taaaaacagt tccttttcct agttctgaat ctacaaatat ttctccacca
     1021 tgctctctta taatggattt gacaatatat agccctaacc cttgcccttt tggctcatta
     1081 ttgtctcttt ctgtaaagaa tctgtcaaat acctttggta tattttctgg aggtatacct
     1141 tttccattat cagaaatttt aataattgca aatccatctt ctttatccat tgataaaatt
     1201 atctttccat caaatggagt aaaggtcaaa gcattataaa ttaaattctc aaatgctctt
     1261 gtaagcatct ctttatttat ctttgcatat atttttttct ccaatacata agtttcaaag
     1321 tttacaccac atgcttgtgt atctggcaaa ttattttcat atatctcttt aataaaatta
     1381 ttcagacata ttttttcact aataccaatg cttttatctt gtgatgtaaa ttcttgcaaa
     1441 ctacgtactc tattttgtac ttcatttgac ttttgattta tagcataaag ataggttctt
     1501 gtctcttcat ctacatgaat attaccaacc tttatcaaat ctataaatgc ttgtatagcc
     1561 gaaacaggtg cctttaaatc atgtgctaca tctgccaaga atcttttacg ttcatttaac
     1621 aatgttgtaa gttcattagt tctcacttca acttcatgtt caagattcct tgtaagtttt
     1681 tcattctctt ttattattat tctattcctg tttatcatca taacagaaaa taaaataaca
     1741 attacaaacc cacaatattc tgtttgccac cctgtaaata taggctcaaa tctattactc
     1801 gtaactgcat caaataatat gctaattcca aaaacagtgt ttactgaaac taaaatataa
     1861 gaacttatat gctcagagca tgtcttttta tatattgaaa ccagcgaaga cattaatata
     1921 tatataaata caatatattt atatatgtct attaacttcc catatatgtt gacaaagtac
     1981 atatcatatg gaagaagaat aattggtatc actatagata tgatactcat tgtaattcct
     2041 aatggtaaaa ttaaaaatct atacacttta ttttcttcta ttttggatat acttccattt
     2101 atacaaataa tacataaaac taccataaaa tatgacaaat cttcaaccgc atatgtaaag
     2161 cttaccaaag gcagtcctaa caaatgtaca aagggatagg atacatggat ggcaaagaaa
     2221 acacacatac atccaaaata taagtaaatt ttacttttgt ttgaaagaaa ccatacagac
     2281 aatgaaaata tagctattgc aaaagttgtg aagcaaatta tactataaaa aagtaatcga
     2341 taaaacatca tattacttat gtcctttgtt tctcctaatg caggcggata atacaaacca
     2401 ctataataat gtgaaaaatt cgatacattg attataattt ctgtattttt ctttgcaata
     2461 aaacttacta ctgaattttt aattttgggt ctatattttt ctgttcctac atctcctaat
     2521 ttagaaacca tttctccatt tatccatact tcacttgcgg aaaaaatctc aggaagctct
     2581 aaacttagta gcttatcctt gccttcattt tttattctca tacgataagt agcattccca
     2641 tatggtgact gagtcccttt tctcatagaa aaatctggat attgacctat aaaaacatat
     2701 tctggtttta tatcttgctc tgtaaaatca ttcggtttaa atattttatg attataaaac
     2761 tcccagtcat ctatcaaaaa gacaagttta cctttagtga aagtatcatt atctatagtt
     2821 aaaattccat tacttgctct aatagactta tatgtatatt tattatcata tttatacaga
     2881 agatttaatc ctgttattgc cagcagagag actatcaata caatgagagt tccttttagt
     2941 atattatact ttttcattgt atcaaccaca tctctttcat agtagtatta aataaaacaa
     3001 gaactattta ttaattacag tatctcactt atggtacaaa cttatatcct tttccccata
     3061 cggtttgaat gtattgatgc tccctgctta aagaattaat tttttttctc atctgtgcaa
     3121 tatgaacctg cactgtatgt tgatctccaa tatcaggcat tttccaaact tggttatata
     3181 tctctatctt tgaaaatact tgttctttgt tctcagctag aaacaataat atttcaaatt
     3241 ccatagtagt taaatctata ctttctccat caaaagttac ttttcttgaa tttacattta
     3301 ttactaacgc tgaaaacttt aacacattag gaactgatgt aatatttttg tactgcctca
     3361 tacgagcata tattctaagt tcaagttctt ttaaactata aggttttgtt atataatcat
     3421 cccccccagc taaaaatccc cgaacttttt cttcttcatg ctcataatta gataaaaata
     3481 ttataggtat gttaaccttt tcttttatat cattgcacat tttatatcca tctatatctg
     3541 gcaatgaaat atccaaaatt atacagtcta aaactatact ctctactata tccatagcat
     3601 ctttagcagt ctctgatgta tatacattaa aacctatatt agaaaaataa atttgattta
     3661 tgtttaaaat atcaacatca tcatctacaa ccagtaaatt atacattaca ccactccatt
     3721 caaaggtatt ttaattataa ttccaagttt tatagttatt acaaattata atgttttatt
     3781 ttcatatata actggtatta ttattttata atatctttac aaactagact ataatactta
     3841 agtatttttg aataattctt gaatattgta ttaaacatta agattcatta ttaaatacta
     3901 attaaagtct ccgattgttg gagaatggag cttaaagtca ttttcacgta taaatttatt
     3961 atttttctag aaacttgtct attggctagt ttgagtattg atttatatga tttatatggt
     4021 tataatataa aataaattgt attattttgg taaatatatt gttacaaaag gtttatattc
     4081 tgcatctcaa tgtttataaa aactttattt tcttattata atactattat tcttttaaaa
     4141 aaatttccct tttccaacaa atggctactt taaaagccaa aattttacct atcaataaaa
     4201 atcttaacga ctaatactct tattttcaaa ttttgcaaca tttttgttaa ttttaaaaca
     4261 aaaaaagact atgcaatata aaaattatat tcatagccgg ttgcaccact aactccatat
     4321 atctctagat gcccaataat aagatatact tctcagtttc taaaatattt ttattaaaaa
     4381 tcttttatat attttcataa atttcttttt tattctaaca ttgtaatatt attttgtcaa
     4441 tatttccata ttcttagaga taagatacta aaatacctat tttatctaaa gatatttata
     4501 tttctacatt caataataga caataccctg caagttaaag catcattttt atgagcttat
     4561 ctcacagggt attatatgtt tactttatat tataactctt gttattgatt aactttaata
     4621 aatccttttt ctgaaacaag tgcttgtcct tcatcactta atatataatc tattaaagct
     4681 ttaccttttt ctgacaaacc atcttcttta ttaactaata taaatggtct tgatattgga
     4741 tatttattag ctattacatt tgcatcagtt gcttcaacat catttatttt taaactattt
     4801 actttctcat caacatatcc aaatgatata tatcctatag cattttcatt tccttcaact
     4861 gtagttttta tatttcctga accatcactt atttgtgcat ctttagttag ttcttctgaa
     4921 tcaaacccta caatttcttg gaatccatct ctagttcctg aaccttcttc tcttgatact
     4981 attactattg gtgcatcatt tcctcctact tctttccagt tagtaatctt tcctgtatat
     5041 atacctttaa cttggtccat tgtcagattt tttactgagt ttgcagtatt agttatcaat
     5101 gctataccat ctattgctat tttagtttcc tttaatccag cacttttttc ttcatctttt
     5161 aaatctcttg aactcattcc tatttcagat gttccttcta tagcattttt tatacctcca
     5221 gaagaaccaa tctgttgtac ttctacacta acaccttgat ttttttcttg aaacttttct
     5281 ccgatagttt ccataagagg acctattgaa gttgaacctg aaacagaaac tttgcttgaa
     5341 tctcctgaat cacctgaacc tcctgagtta gaactacatc ctactgctaa agctccaact
     5401 aacattgtac tagctaaaag aacgcctatt ttctttttaa acattttact tacccccgct
     5461 tatattttaa ttttttgttg tcatttttct atattttaat aatacagatt ctttgttaaa
     5521 tgaatattag agcagggtta agtaaatgta aatattgtta aatacatgta aaaacttaat
     5581 tttatatgat ttattcttaa ttagattaac tttttaaagt tactttaggc agatataaac
     5641 ataatacaac tcatattatc agaagtcttc attgaagcta tcaacctatc acatcctata
     5701 attctttttc tagtttatta actaattcag caaatacatt taaggcttcc tctatagatt
     5761 cttttttcgt catatctaca ccagcagatt ttaattgttc tagtgggtat tctgaccctc
     5821 cactttttaa gaagtttata tatctatcta ctgcagtact tccttctgaa agtatctgtt
     5881 gacttaaagc acttgcagca gaaaaccctg tggcatactt atatacatag aagtttgaat
     5941 aaaaatgagg tattctcgcc cattctattc ctatttgttt atctactatt gcagattttc
     6001 cgtaatactt ttcatttaat ttgtagtata tattagtaaa ttcatcagca gtaagtggtt
     6061 ctccactctc taccctttga tgtacaattt tttcaaattc agcaaacata gtttgtctat
     6121 gaactgtagt tctaaattgt tctaaataat aatttaataa gtatatagtt tcatccttag
     6181 acttagaatt ttctaataaa taatttataa gaagtaattc atttaatgta gatgcaactt
     6241 ctgcaacgaa tatcttatag tccgaatata aaaatggttg acttgttctt gaataatgac
     6301 tgtgaacaga gtgccctaat tcatgaacta aagtgaatag agaattaagg tcatttttat
     6361 agctcattaa tatataagga tgtgaatcat aacttcccca agaataagct cctccttttt
     6421 taccttcatt atcatataca tcaatccacc cttcatcaaa agcacttttt atgaccttca
     6481 agtattcttc tcctaaaggt ttaagtgcct ctaatatgat atcctgtgct ttatcatatg
     6541 gtatatccat gtcaaattta tctgtcaaag gaacatacaa atcatacatg tgtatttcat
     6601 tcaatcctaa aaatttcttt ttaagctcaa catatttatt tagattagga atacttttat
     6661 ctatagctga tataagatta ttgtaaacat ctaaacttac atcatctgaa aataaagacg
     6721 cttctattgc tgaattatac tttctagttt ttgcgtaaaa tatctctgat tttattccac
     6781 cataaagcgt tgaggcaaat gtatttttat atttctcata tgttgaaaat tcaccttcaa
     6841 aagcgtcttt tctaactcta ctattcttac tttttataaa taaagagtag tttgaatgag
     6901 aaagcttaac cttattcccc tcctcatctt ctatctcagg aaattccata tctgcatatg
     6961 ctaacatatc gtatacattt tctggaattg tcgataattc tgatgtagct gcaagtattt
     7021 cttcctctcg ttcactcaaa gtatgtggtt tatctctcaa tatatccttt atatattttg
     7081 tataaaagga tagcttctca tcttttaagt attcacttaa cttattctct tctatcgcca
     7141 gtatttctgg aactatataa gaagtagcca cacctaaatc agtagataac atttctgtct
     7201 tagtggctct tccttgatta tcattaattc tagtatcttc atgttgtttc atatgagtat
     7261 atacatataa attttgaaca atcctagatg cattttcaga gatatttaat actttatata
     7321 agttctcctt actctcagaa agcttccctt tatattcttt tacttcttgg ataagatttt
     7381 ttactttact tatgtctttt tcaatttcct ctttactgga gtacatctta tctatcttcc
     7441 atttgtactt ttcttctatt gtacttctat cagttcccat atcatacacc tctattttaa
     7501 tttgttttat attttattca ctttacaaaa tatattgtat atattttagt ccaaataatc
     7561 tacatttatt actccttcta tcatatttat ttctttcata acatcgtgta agtgtattct
     7621 cttagcataa gatacaaaaa aattaacctt ccagtattct tcattacgac attctatttc
     7681 catatttctt acgataagtt gatttttttc aaatttatca tataaaaaag gtatagagtc
     7741 caaattgctt aattttaata tcaatacatt cttttcttgg ctaaataaaa acgtattgat
     7801 tatcctaagc acaaataatg ctatcattac aaataatcca cctagaattg ccaattcata
     7861 ataaccatat ccaactgcta taccaataca tgcagttgcc cacatcccag cagctgtagt
     7921 aagccctttt accccacctt tattttttaa aattgctcct gcacctaaaa aaccaattcc
     7981 agataatact tgagcaggca ttcttgctgg gtcagcattt gtatacaaat tataatcaaa
     8041 gaataattga atcgaagtta ttgaaacaat acaagaacct attgcaatta gtatatgtgt
     8101 cctaaatcct gcaaactgtc ttattttttc tctctctaag cctattattc ctcctattat
     8161 tagtgctaat agtaacctta ttgctatctc cttacttcct atatcaatca taatataaaa
     8221 ccctcctgac ttaattacaa cttatgtcct acagataatt tacacatcag aaatactatt
     8281 ttatatctta atattattat taatatatgt aaaatatagc catctgttaa tttttgatac
     8341 aaaaaaaccc acaactgtgg gctatttgct taggtacatg tatttattta ttaatatatc
     8401 tattttctta cttgattcta ctaactcttc ttgaaatatt tctggatatt ctatgtatgt
     8461 atttatctct tccctaactg cttcaatctc attttttaac gcttctaatt cattgttcgc
     8521 cataataatc accactatta caaatttgta tatttttata tacattttat caaacaatat
     8581 tatattttgt caatattatg tcaataaaac ttactataaa aatgctttaa tttaggtatt
     8641 gtatacctgg atactaagcc tacaaacata ccagttacta atgatgccat taacataaat
     8701 ggtaagtacc ctatagcaag tatattttct atgattaaac ttgcaactat taattgacct
     8761 atattatgcc ctattgcacc tataatgctt atccctatta gtgataaatc ttttatttta
     8821 ctcgctaaat acataaataa aagactcaga taagctcctc ctatactaaa tagtagatat
     8881 gataaagggc ctgcaaatat tgatgacaaa attaccctga tagtaacaat tataacagta
     8941 tctttgacac ctaacaacat taatgaaatt aaagttataa tattgctcaa acccaatttt
     9001 gcccctggaa atataactat aagaggattt ggtatatatg tctctaatac atataaagct
     9061 aaactatacc ctatcataag acctaaaaat gtcatttttc tagttttcat taagttaact
     9121 cctccaaatt ttatctatca tcttacatta taatacaaaa tatataaaaa cacctaattt
     9181 agactaaaat atttttaatc tatttagaat aaatattagt tatttcaatg tgcaattacc
     9241 tctataaatc ttgacttttt gtatcatact tgttacaatt ttaatgataa tgaatttcat
     9301 taggaggtgt taataatgac tgtttacgat ttaaaaatag gagaaaaagg atttattaat
     9361 agtatagatg gagatattaa attatctaaa agattgcttg cacttggatg tataaatagt
     9421 actgaaatag aagttaaaaa ggttgcccca cttggagacc caattataat tagatttaga
     9481 ggctttgacc ttgcgattag aaaatctgat gctaaaaata tttctttaca ataatagagg
     9541 aggagctacc cgtgattaat gtagcattat taggaaaccc aaatgtcggt aaaactactg
     9601 tttttaatct acttacaggt tcaaatcaat atgtaggtaa ctggcctggt gttactatag
     9661 aaaaaaaaga aggatttctt ggaaaagaga taaaggttgt cgatttacct ggtatatatg
     9721 caatggatac tttctcaaat gaagaaaagg tttctaaatc atatttagaa aatgaagatg
     9781 ttgatgtaat agtaaatgta gttgatgctt ctaatttatc aagaaacctt tacttaacaa
     9841 ctcaacttat gcaatttaac aagcctatag ttatattgct taatatgtta gacattgcag
     9901 aatctaaagg agttaatata gatgccaaaa aattaagtga agaacttgga gtaattgttg
     9961 ttccaattat agcaaaaaag aaaaatggtg ttgataggat agaagaagag attaagggag
    10021 ctacgcaaaa agcttttata tatcagaaaa actttggaag tgaaacagaa acatataaaa
    10081 aattagaatc cattttatcg agatgtacaa aaactacata tacacaaaag aaatctatca
    10141 gtgataagat agacaatata gtcttgaatc ctatattagc atatccgata tttattggtg
    10201 tattgttcct cttatttaaa tttacctttg actgggtcgg aggaccacta caagagggat
    10261 ttgctagtct tatagaggct tatatttctg cacctgttag tgatatgtta tctaactcta
    10321 gcccatggtt taaatcactt atagttgatg gaataattgg aggtgttggt ggtactttac
    10381 cattcttccc tttaatattt acattattct ttggtatatc gttgtttgaa gatagtggat
    10441 acatgtcaag aacagcattt ttaatggata aagttatgag aaaggttggt ctatctggta
    10501 aagcttttat acctatagtt atgggtatgg gatgttcttc tccagctata atggcaacta
    10561 gaacattgga aagcgaaaaa gatagaagag ttacagctct tattgcacca cttatgactt
    10621 gtggagcaaa gctacctata tatgcactat ttgtagctat attctttcca cataatgctg
    10681 ctttagtaac tacatcactg tatttggtgg gtatagttat ggcaattgta gttgcattat
    10741 tcttaaataa aacagccttt aaaacagagg ctgaaccatt tatattagaa ttaccagaat
    10801 atagaatccc tactatagat gcacttatta aaaatacttg gaataaatca aaaggtttct
    10861 tgataagagt tgttactgtc atgtttgcaa tgtcagtcgt aatttgggga ctttcttcat
    10921 tcaatgtttt tggatataca gaagatataa atgtaagctt cttggctact ctaggtcata
    10981 ttatatctcc tatattcaaa cctcttggtt ttgatgattg gagaacttca gttgctattt
    11041 taagtggtct tggagcaaaa gaaatcgtag taaactcttt aaatatacta tatggtaatc
    11101 taactgttgt cttaccaaca atatttaatg gagttactgc atatacattc ttgatattta
    11161 ctgctttata tactccatgt atagctgcac ttgcaactat gaaaaaagaa tatggcaata
    11221 aaatgatgtt tacatcattt gcatatcaat ttatactagc atggataatg gcttttatag
    11281 ttaaaagtat aggtggagta ctatttatgg gtaattcaat aatggaagct ttgataggtg
    11341 gaataattat agtccttgca ctctttattc tgtttaataa attcaagtct acaaaaaacg
    11401 gaaatgtctg tagtggatgt tctatttgtt ctggatgtcc aagtgctaat tcttgctcaa
    11461 gtaaggaaga cagcaataaa aaaaataatg cacactaata tataaatatt ttagtttaaa
    11521 ataagataaa agtgggtgtc tcaaaattaa ctttttagtt catgaggcac tcttttttat
    11581 ataaaatcaa atatattttc atcatttcaa cagtaaaaaa gagcttatct agtaaaaaag
    11641 aggcttatct ctattttgag acaacctctt tttttgttac ttattcagtt tcttgctacc
    11701 taacttcatg tggaacctag tatcttttgg aacacttggg tctacacctc ttaatgtagg
    11761 tatttcccca catattagtt gtataggtac tataaaatta aatgtttgat agtaaggatt
    11821 atctgtgata tcaatcttta aatttttatc attttctgta acatctctac ctatagcaaa
    11881 tacattttcc gtccaaccag aaagtacatc aatcatctta gttactctag gttcttttcc
    11941 tgtatctagt ataaatatag tagaatcact atttatagca ttatatatac catgtataaa
    12001 ctcttcaaac tcatacccag ttacaggtat tctcattgtt tccaataatt ttaatgctgc
    12061 ttctaatgta tctccatata tatcagagtg acctattatt cttatttctt tagaattaac
    12121 taacttttct ttgtttcttt caatccattg tttagagagc ttatacactg cttcaaatct
    12181 attaattgcg tctaatatct tatttatttc ctcattgtac ttttcactag atattatacc
    12241 tttttctcta gctatttgaa gacctaataa cattaagttt aactttgtac aataatatcc
    12301 ttttgtttta gctccagatt tttcttcacc acaatttact gttaatatat aatcagaaat
    12361 ctcatctatt aaagcattct tacaacctgc catagatgct attttacagc ccttatcttc
    12421 agctaacttc atagcgttgt atgtagaata actactccca ccttgagata cacctactac
    12481 tagggtattt tcattatcaa acttgaatgt atcttcagtt atcataaatg gatacatttt
    12541 cacaacatct atatctaata aattttgtaa atatggttgc acctgcactc ctgagtgata
    12601 gctagtacct gaacctgtta ttataatctt ttttagattt gttctcttaa cttcattaaa
    12661 taagctatct gcattagata taatttctct cattctaact ggtgtttcta acatataatc
    12721 ttgtattgtc attttataca ccttccttat ctatatattc aataaattat aattaatttc
    12781 tataacaaac ctatagcatg taaagcgatt cctgttacta tagtgcctat agttaatttt
    12841 gttacactta cctttctctt taacatgtag tatattacaa atgcaaaggt taaaggtaac
    12901 atattaggta ttattttatc tagtatatct tgtaaaatta cttctgcacc atttaagttg
    12961 aatttcagag gagtagttat actcaacata gttgcaacca ttcctcctac aacgaataaa
    13021 ccaacagttg tagccataga cattattttc tccataagtc cctctctttg tattctttca
    13081 agagaattta cccctacttg ataaccatac ttaagtccaa atattctaaa ggcaaatgct
    13141 ggtatattgt ataatagtaa gaataatagt ggtccaaaga tattaccttc ttttgcaagt
    13201 gatactccta ctcctgctgc tataatccta aatgttcccc agaataatga atcaccaatt
    13261 cctgcaagag gtcccataag agctgtttta acagcactaa ttgaactttc atcaaattct
    13321 ggattattag cattctcttc ttccatagcg gctgttatac ctaatatagc aggaactacc
    13381 actgtcgttg tattaaaaat ctccaaatgc ctttctattg cttttgcttg attttctttc
    13441 ttattgtata gcttctttat tgcaggtaac attgaataac agtaacctaa gttttgcatt
    13501 ctttcgtaat taaatgcacc ttgaagagca aatgaacgcc aaaacatact tcttaattct
    13561 ttttttgtta atttattcaa agtcaatctc tccttccgtt aaagtattga ttgctaattg
    13621 aggattttga ttattttctt tgttattgat ataaaagttt agtacaaatg ctacacatac
    13681 tccaaataaa gctatagcag ttatatctaa tccaccataa gatgctaaca aaaaccctat
    13741 aaagaaaaat acagacatct tttttgaaag taacatatcc ataagcaatg caaaacctac
    13801 tgctggcaac aatgttcctg caacacccaa tccgtctaat ataactttag gtattgcatt
    13861 tattaatgct gtaactttat cagcaccaat cataattgct aaaaaggttg gaactgatgt
    13921 acttaaaaag aataatatta ctggcaacca catcattatg gtaactttct taaaatcagc
    13981 ctgtttagca tagaaaactg ccttttgaga aaagtacgaa tttattattc taactaatac
    14041 tccaagagat tgtgctaaga ctgctactgg tactgctact gctaaagcaa cttctgcacc
    14101 attacctgat aatatagcaa aagctgtacc taatacacct cctgttacta catctggagg
    14161 agtagctgca cctattcctg caattcccat ccaaattaat tcaagctgtg caccaatcat
    14221 aattcctgat tttatgtctc ctaaaactaa tcctactaac atacctgtaa ctattggcct
    14281 atcaagcata gattgaccaa atatacgccc atctagtata ccaactcctg ctataatagc
    14341 taacatagta gcttgtacta acatattgaa cctccatttt aacttagttt aatttacatt
    14401 aaattacatt acatttttga ttttgttttg attttctaat aactcattta cataaacaat
    14461 tccatttttt gcttcctcta atcctgattt aactattagt tttgtatcct cctcaactaa
    14521 taaaagtata gataaaagca ttggtaaatt tactcctgtc aataattcaa atttgaaccc
    14581 ttgtaaaagt aagtttgtac atatgtttgc cacacttcca ccaaacacat ctgttaagac
    14641 aacaaattct tcactatctt tattatttga taatatactt ctaatttctt tctcagcatc
    14701 atctgctgat ttatccgttg tcatacaaaa atactcaata ttagttaatg aaccaattat
    14761 taatttactt gtgtcaacaa gctctctgga caattctcca tgtgttgcaa ttagaaattt
    14821 tttcattctc tttctcctaa attattttat taaatctgat agttgtttct ttttgtctct
    14881 aggtaacatt ctaaattcta tatcaatccc taaatcacct atttgtttaa agttatttat
    14941 atcatcatca tcaacagcaa ctaaatctgt aagtttcttc ttcccttcct catatcttaa
    15001 tcctcctaca tttatttctt ttaatactcc tcctgaattt ttagctacaa ctaaagcatc
    15061 tttggtattt tttactaaca ctaatacatt gcttttcttt gaagaagcaa atttttctac
    15121 tatttctcca gattcctcta ccccccttat atataaagtt acattgctag gtttcgctaa
    15181 gttcagtgac atagctttca tttggtcatt ttttgcttca tcatttgcaa ctagtattac
    15241 atttatacct agtgatgttg cccatccata tgcaacctga ccatgtatca atctgtcatc
    15301 aattctcatc aaagatacca ttttccccaa atcctccatc atacattaaa atttttattg
    15361 tttatactct catttatcta taaaagcaaa aagtatgcca aatactggca tactttttta
    15421 aatttcattc acttattttg taatcttgta ttttactgtg gtagaccata gataatatta
    15481 taaatatatc ctatctctga aattggtatt tgcacactat aactcttttc tagcacacta
    15541 aatgcatttt ttattagttt tacttcacta tgatggtttt tgattaacaa atccatatca
    15601 gtatactctt caatttcagt ttgtctaact aatctttcaa tcatacaact taagtgaaca
    15661 tataaagaaa tctttttatc atttgaaagt tttttctttt gaatattttc tatagcatta
    15721 attccttctt ctattctctc tattatcttt tttgaatcta aaatagttag actatctatt
    15781 actctaatta atgaaaaatt ctttacaata gaatcattta tgttctttat tttttcattt
    15841 tctactactc tgctaaatat tctaaaaaca tcatcttctc cttttcctga tattatatcc
    15901 tctaataata taaaatctac cccattcaca tttggattgg cagttcctac tatagccatc
    15961 acatcataca tttcaaaaac tggttcttta tctcccatag aaacaagact atcataatca
    16021 taaggtatta ttttgacatc aactattcca attaaacttt cggaaagtag atgttggatt
    16081 tttatagcaa tacctatccc tgtaaaacaa gatgatatta tagcatattc tttatttttt
    16141 tgaggataat acaattgtat attaggttta gtttcttcat ccataatttc tgcaatctct
    16201 tcaatgtttt taccttgcat taataaattt cctacttcta gtgcagaaat agtggatatt
    16261 tggtctataa ataacattgg gctatttatc ttattcttta aatcttcata tataagatta
    16321 agagagccca tatcaaataa tacaattaaa ccattcttta tcctatctcc ctctagatat
    16381 ctagacattt gttttgatac atcagcaata gtagcatcta taggcatatc aatagctgta
    16441 aatatatttt tacccaacat ccgattacat acattcgcca tactactggc agtagcatat
    16501 ccatgtgcta taatagtcgc attaacttga ttaaatttca ttcttataat tgttgattta
    16561 ataaatataa tcattatgat tgtatctaca ttattaagat acatatccaa tctattcgtt
    16621 attaatgttg atattttact tattatagag tattctttgt aactctcttt ttttaaatat
    16681 attagtaatt tattttctaa agctttatac tcatcagaca attgataaga ttcgtccttc
    16741 aaataaagat aagacgaaaa tgccaataca ttattcccat catattttat attgttatta
    16801 acttctaaat ttctaaatat atcctgcatt atcgatatta taagctccat aagtacgtta
    16861 ttattttcat cattatttga aaagactaaa ttgtccatta gtctaatcat tagattagaa
    16921 ctctttttac taaatttttc ctttgttatt tcattttttt ctagtctatc aaagtaatct
    16981 aaacatttta tgaaactttg atagattata tttcttttat ttcttccatt tgacatttca
    17041 atactaaatt gtttaggaga aaagataatg cttttgcttt taccactttg gaattgaaat
    17101 atatctacat tttctatata aagatttcta ggaaggtctt gaagagtaac aaaaatattt
    17161 ccatttcttc tttgattttt tgcataagct cgtccacatg catattttat tacattttta
    17221 cattcaccta tattcccctt gaattttatc tttaaaatcg catttaatgt aatttgagtt
    17281 atctctatag gcaaaccaag taccttactt tcttcataaa taaaatgttc tacaatcttt
    17341 cttttttcaa tagtccctct ttcttctata ttgggaacat aaatatttat gggtattctt
    17401 cttaaaaagg tttgtaaaaa ttcagataga ctttctgttg tagcaaatat taatcttacc
    17461 ctagcttttc taattactcc attctctcca atcctagaaa acgtacctgt atccataaat
    17521 gtaaataact tttcctgacc ttcactattt aatctatgga cttcatctaa aaataaaaca
    17581 ccaccatctg attgttctat tagacctgct ctagattcat ttgctccagt atatgctcct
    17641 tttacatgcc caaataatag actagacaat aactccaaat tattataata ctgcgcacag
    17701 tttaacacat tgtatggaga atttttttct aatacattac tgcttacact atatttgtat
    17761 attatcttgg ctaaaaaagt ttttcctact ccactttcac ctgtcagcat tattgtaagt
    17821 ccgccatttg gataaagtac agcggttttt atttgttcga ttacttcttt taagctatta
    17881 tcatatccta tgatactatt aaacacagtc tcttgcttta ataatttaca ctcattttta
    17941 agttcatcta aattttcata aatacttttt tttgtaggtc ctactacatc atttatttta
    18001 tttttaggta aaaaaataac aggtctactg tttatcttta taagattatc agattttact
    18061 aaatcattta ataatctact tgattgagtt ctacttaaat taaacttttt tactatatct
    18121 tctgttatta tgccactttc ttttttaagt tcatcaaagc tgttaaataa tacacattca
    18181 tttacaaaac ttaaaatttc atccctcatg ttcaacctcc aatacaaata tcaataatac
    18241 tataatatta gtatatcatt tatatattta tgaaaaagat aattatattc ctatcttgat
    18301 tctcttttgt aaggttgtaa aatcaaactt tttataagta taaaatctag attttttaac
    18361 ataatctcaa ggttcttatc tttccatata tctgacatag aatttatata agaatatatt
    18421 tattgtattt attataataa aaagtaaaac attataggta ttttaaaact tattagcgaa
    18481 gaaaatattt gtaactttta ttaccttatg aatattattt caaatttaaa aagg
//
LOCUS       DAMJVI010000078        18470 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.080, whole genome shotgun sequence.
ACCESSION   DAMJVI010000078 DAMJVI010000000
VERSION     DAMJVI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18470
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.080"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            158..1318
                     /locus_tag="PC545_002648"
     CDS             158..1318
                     /locus_tag="PC545_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HDF2457411.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGTYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGADSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPKFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISKLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLA"
     gene            1661..1822
                     /locus_tag="PC545_002649"
     CDS             1661..1822
                     /locus_tag="PC545_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457412.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2015..2644
                     /locus_tag="PC545_002650"
     CDS             2015..2644
                     /locus_tag="PC545_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDF2457413.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLDCDVICLQEVLYPQ
                     FLALKAVLNMGGVFATNVKKVNMIYGICTFTTSKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVILCGDFNEKNVFLSMFNDMA
                     ISLNKSYLPTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2884..4143
                     /locus_tag="PC545_002651"
     CDS             2884..4143
                     /locus_tag="PC545_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HDF2457414.1"
                     /translation="MGRRTTRVSRLGKEKKRSVLIVLAALVLIMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKAEALKAITDKYTPEDLNLAYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLSYTDSVVGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLSGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4402..5397
                     /locus_tag="PC545_002652"
     CDS             4402..5397
                     /locus_tag="PC545_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HDF2457415.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNS
                     EEKMLQSIDKGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVIQVKKEYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5643..6710
                     /locus_tag="PC545_002653"
     CDS             5643..6710
                     /locus_tag="PC545_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDF2457416.1"
                     /translation="MSNIKIIASNKCWIEDIARKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGVLKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYDKDGLEVESEKAIDYIKKHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTEISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICNDVNLLFQEAPEAYKNIEQIIES
                     LIEYELIQVVATMKPLITYKG"
     gene            7244..9154
                     /locus_tag="PC545_002654"
     CDS             7244..9154
                     /locus_tag="PC545_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDF2457417.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLTPSGNIGVVR
                     RKLAENKEARKIIENSNHIPLEGLFNAGSTIDKIEKGMIIEPVELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKSIEDEINYCIKSNKVDSNASKELKKIRRNIEIT
                     EGKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVNGTILDTSSKGNT
                     VFIEPISVSNLSTELTMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMSLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNIEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPMDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDVLFTSFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9217..9840)
                     /gene="ytaF"
                     /locus_tag="PC545_002655"
     CDS             complement(9217..9840)
                     /gene="ytaF"
                     /locus_tag="PC545_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HDF2457418.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFLLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLLGCLLGNMFVKNVNLNLSWLSGLILLVIGFMKIC"
     gene            10028..10672
                     /locus_tag="PC545_002656"
     CDS             10028..10672
                     /locus_tag="PC545_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457419.1"
                     /translation="MEQHSKVGNFGDLLKVLLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFRNSNNQIFNADSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10886..11944
                     /locus_tag="PC545_002657"
     CDS             10886..11944
                     /locus_tag="PC545_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HDF2457420.1"
                     /translation="MNCKWISKIDERKKCHREADSSGYCIFHKENKSDEEIQLMMDTL
                     HKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FRDKVLFNGCVFLENCDFNRTIFSKHYINDRIFEKVKFKGPDLVVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIAIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFKMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12048..13232
                     /locus_tag="PC545_002658"
     CDS             12048..13232
                     /locus_tag="PC545_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDF2457421.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHVCKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDHYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGTKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTNSGDLLGSVDIVSNNSIDNVSLKTKLKMKF
                     AN"
     gene            13439..14152
                     /gene="rpsB"
                     /locus_tag="PC545_002659"
     CDS             13439..14152
                     /gene="rpsB"
                     /locus_tag="PC545_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDF2457422.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14231..15142
                     /locus_tag="PC545_002660"
     CDS             14231..15142
                     /locus_tag="PC545_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDF2457423.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15227..15931
                     /locus_tag="PC545_002661"
     CDS             15227..15931
                     /locus_tag="PC545_002661"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDF2457424.1"
                     /translation="MDKPMYKRVLLKLSGEALAGERGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15957..16514
                     /gene="frr"
                     /locus_tag="PC545_002662"
     CDS             15957..16514
                     /gene="frr"
                     /locus_tag="PC545_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDF2457425.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEEFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16517..16720
                     /locus_tag="PC545_002663"
     CDS             16517..16720
                     /locus_tag="PC545_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457426.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQNINYTVKSIEGKKDKEKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16749..17483
                     /locus_tag="PC545_002664"
     CDS             16749..17483
                     /locus_tag="PC545_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDF2457427.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17496..18275
                     /locus_tag="PC545_002665"
     CDS             17496..18275
                     /locus_tag="PC545_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDF2457428.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVHPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINGFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7591 a         1849 c         3220 g         5810 t
ORIGIN      
        1 aaatctttat taatatatgt caatagttag gtatatatgt atttaattag catgatatag
       61 ataaaagtat ttattttaat tgtctattat ctgaaaaagt ctttacttag aaatcataaa
      121 aatcaaatta attaggaaaa gaaagggtgt gtataatgtg attgagactg gatttgtata
      181 ttgtagtttc ttggttgtat tcgtaggtgt aatttttttt atggttgata aattgaaatg
      241 gaaggtattt gatgttatta atccaatgct tttaatatac ttaggttcag ctatacttgg
      301 atcatttggt ttatttgcac agaatgaaga tgttggaacg tatcaaaaca taataacagt
      361 gaacttcctt ccattaatgt tagtgttttt gttaattcaa tgtgatttaa gaaaaatatt
      421 aaagatgggg cctaaaatgt tattatcatt tttctgtgct acatttacat ttatgcttgg
      481 atttgttata gcattcataa tttttaaaaa ttcaatgggt gcagattcat ggaaaattct
      541 tggggcagca tctggttcat ggataggtgg ctcatcaaat atgattgcag cagctaatgc
      601 tctaggagtt gcagaagaag gacttagtta tgcaatactt atgggttcta taggatatac
      661 agtatggatg tctgtttgtc taatgtctgt gaaatttgga cctaaattta acaaatggac
      721 aaagtgcgat acaagtttta tagatgaggt tacatcaaaa cttgaaaaag aaaataaaga
      781 taatacagca cctacgtttg tagaacttat gattttactt tctgttgggt ttggagttgc
      841 gtcttttgca caaattgtat ccaagatact tccaacttca ggtatactta atgaaactat
      901 gtgggtagta cttgttgcat ctacagtatc aatattattg ggtatgacaa aaatatcaaa
      961 gcttaaaggg agtacactgg taggaaatgt gtttatgtat ttactccttg cagctacagg
     1021 tagtaaagca aatttctttg gattgtcaga agcacctgtt tacataatgt ttggattggt
     1081 agtagtcctt gtacatgcta tttcatttat agggcttgct aaactattta aacttgattt
     1141 atttacttgt gaagtaggaa gtatagctaa tataggagga gtaactacag ctccaatgat
     1201 tgctggggtt cacaatcctg tattaatacc agtaggtgta cttatggggc ttctaggaaa
     1261 tattataggg acttattgtg ctcttatggt aactcaaata ttacatatgt tagcttaatg
     1321 aaaatatggt ataaaattaa taatttaaat aagaaaagtc atttcaattt aagcagaagc
     1381 ttttactgaa atggcttttt ttgtttgaca agataatcta tttcaagaag atgttttgac
     1441 atatttagtg tataatattg taaaatttac taaaaacgag atatatttaa tttttatatt
     1501 gaatcgaacg tctgtatgat gtataataaa tatacagaac gaatgtttgc ggaacgggtg
     1561 ttttttgaat ttattttaat aatttatgaa tatatatcag aatgcataaa tatatttaaa
     1621 tataaagatt aaaaagaatt taaaggagtg ttgataactt atgataattc catcaagaag
     1681 aatacaagat ataacattaa caaatttaaa aaatggtgaa gtaactttaa ttgaattgga
     1741 tgaaatatac aagaagatgg gatttttatt tgttgttagt gaaggcaaat tgaaaaagat
     1801 aaaaaaggag aatcatcatt agaaatgaat gaaataataa atattataat agtttataaa
     1861 acctaactta tgtagaatat aaatacttaa gttaggtttt tttgtgataa agtttataag
     1921 tactcaattt tataagataa ataatggttt taagtgaaaa gtaaataagt ttaaatttta
     1981 gtaagtatta tattgcataa gaggtgatat tattatgaaa atagtaacct acaatattca
     2041 taaaggtatg gattcaaata atagattaac tctaactaaa atggggttat atttaaaaca
     2101 attggattgt gatgtaattt gtttgcaaga agtattatat cctcaatttt tagctttaaa
     2161 agctgtttta aatatgggtg gtgtctttgc tacaaatgta aaaaaagtaa atatgatata
     2221 tggtatttgt acatttacaa catctaaaat gttaaacagt aatcattttt tcttaactag
     2281 caaaaaagaa caacggggag ctctttgtat aactatagat gcatatggaa gaattataaa
     2341 tgtaataaat actcacttgg gacttgatag gcaggagaga gcaaaacaat tagacgaaat
     2401 aatagactat aggaacagat tagtggggat agttatttta tgtggtgatt ttaatgagaa
     2461 aaatgtcttt ttaagtatgt ttaatgatat ggctatttca ctaaacaagt cttacttacc
     2521 aacatttgaa aaatcaaact ctagaataga ttacattttt gttaataaaa atactgaact
     2581 aaaaggatac acagtagaaa aaatttacct gtcagaccat tatcctgtaa taggttacat
     2641 ttaaatttca atataaatga aattagttgt tataaaataa acaatttttt tgttaagaaa
     2701 tagtaaaaga aatataataa aaaatatcaa aaataaaact aattataaga cataaattta
     2761 caataaagtt acatttgatt aaattccttg tttaacaaaa gctattcaaa tataatctaa
     2821 ggtagttttg aaatgggaac tctattatgc aaatttaagc tagtgttaaa atgaggtgaa
     2881 gttatgggca gaagaacaac aagagtttca agattaggta aagaaaaaaa acgaagtgtt
     2941 ttaattgtat tggcagcttt agttctaata atgggtggat ttacttattt ttttaacagt
     3001 aaatttctgt acaatgggaa aatcgctaaa aatgtatata tagaaggagt aaatgtatct
     3061 gatatgacta aagcagaggc attaaaagct ataacagata agtatactcc agaagactta
     3121 aatcttgctt atgatgggaa aaaatataca atatcaccaa aagatattga tttaaaatat
     3181 gatacagaag atgttgtaaa agatgcttat gaaagtacaa aaaaagggtc ctattttcaa
     3241 aacttaaaaa aatatattga tataagagtg aataaggcaa atatgaagat aaaagctgaa
     3301 tatgatgagg caaaacttag ttccaaagtg tcttctatag cagatagtat aaatgttaag
     3361 atgaaaaatg cttctattag tgttggaagt ggtggactaa gttatacaga ctctgttgta
     3421 ggtagagaat ttgatttggc agcaaataaa gagtctatat ataatatgat aaaaaataaa
     3481 gagcataaaa ctttagaact taaagtaaat cttcaaaaac cagatataac aactgaacaa
     3541 gtaaaaactg taaattctgt tataggtcaa tattcaacta cttattcaca agctgtagag
     3601 ggtaggtcat ataatgtagg attatcagca agaaaaacta gtgatgtatt attaatgcca
     3661 ggtgaagaat tttcatacaa taaacttaca ggacctagta ataaagcaaa tggttacaaa
     3721 gatgcacctg ttatagttta tggtaagcta gaacaatcag caggaggagg tgtttgtcaa
     3781 acatcttcaa cagtttataa tgctgctctt ctttcgggta tggagataac tcaagttaca
     3841 aatcattcat ctgcatctac ttatgtgcct aaagggagag atgctactgt gagcgatgga
     3901 ggattaaact taaagtttaa aaatccatat aagcatcctg tatatattaa aaattatgca
     3961 ggtggaggaa gtgtatcatc agtaatctat ggtaattctg gtgacaaacc aaatatatct
     4021 atagaggtaa aacaaactgg tcagaataaa tattctacat atcgaatatt taaagattca
     4081 agtggaaagg tcataaaaaa agaacatata tcaaatagtt cttataaaga attaaaaaaa
     4141 tagataattt tttagagaaa tatttgtaat ataaaaggga gtgtctcaaa atgaactttt
     4201 tagtttatga ggcactcttt ttctgtgaaa tcaaatatat tttcatcatt tcaacaatac
     4261 aaaagaagcg tatctctatt ttgagacagc cttttttgta aaaattaaca ctattagttc
     4321 ttgttttaat attacaatca ttaatatata gaaaatattc ctatatattg gtataattgt
     4381 aaatagattt agttatataa aatgtatgag gaggtgacat ggttgaattt tataggcaat
     4441 agatatgagg tagtaaattc tgatgacgtt ttagaaatta acaaaatcta taaagctaga
     4501 gatgtttttt acaggaaaaa tgtgttaata aaagttataa agcacaataa ttatatctgt
     4561 gaagattttg tttcaaattt aattgatgaa agtactgctt tagatgagat aaactctcct
     4621 tatatactaa aaataataga tgttgggatt cactgtacag aagaaacaac attatattat
     4681 gttgtaagtg aagactttaa tggtatcgga ctagatgaat taattctagg taattatctt
     4741 catttagaag caattgtaaa cataatgata caagttttaa aagctttaga aatgatacat
     4801 aggaatcatt catatcatgg tagtttaaaa tcgagtagta taatagtaga tacagaatac
     4861 aatataaaaa tttgtgattt tggaattaca aaagcaaata atggggttaa tacaagaagc
     4921 tatggaaaca ggagatattt atgcccacat caactatgta taaattatac tgataaagaa
     4981 agtgattttt ttgcaacagg tcttattcta tttgaatcta tatttaaaaa attgcctttt
     5041 ggagaatcaa attcagaaga aaaaatgcta caatctattg ataagggatt agattggaat
     5101 agtataaaag caattaatgg aaatactgaa cttataaatg ttataaaaag gcttcttgga
     5161 agaaataata agtatactag agccaatgat attattattg atttaagtaa agtaatgtat
     5221 gaaaaagcat atatagaagt tgaagatgag aaagaagaag agcagaaaca agacaaagtt
     5281 atacaagtta aaaaagaata taaaagtaaa atgagaaaaa aattacataa aaaacttgtt
     5341 gttgccggtc tagctatagt catgatttca atgattatta ttagttccat aatataaaaa
     5401 ttgtatatta agtattttaa aatcaatgtc tataaaggat atacatagaa attttagtgt
     5461 agtcttaaat tatgaaagga agtggttatt atgttaacaa aaaataatga ggtattaaac
     5521 gtctaggagt attttaacaa atagccacct gcttttacag tttagaataa aaaagctgtg
     5581 gctaaaggtc acagcttttt gctacttttt aaaggcagga gaatattaaa aaggagcaaa
     5641 ttatgagcaa tataaaaata atagcaagta ataaatgttg gattgaagat atagcaagga
     5701 aacaacttga agatatttca aagttaaatg gaattttaag agtagtagga ttacctgact
     5761 tacatgcagg taaaatacct gtaggtttag cagttgagac taaaaatatt atttatccac
     5821 atataattgg aaatgatata ggatgcggaa tgactttatt taaaactggg gttctaaaaa
     5881 agaaatttaa aaaagacaaa tggattaagt cacttagtaa gattaaagat ttgtccgata
     5941 tagaaataaa aaacacatat aaagaagaat gtccaatttt aaatttagga actataggga
     6001 gtggaaatca ttttgtagaa attcaatgta taagtgaaat ttataataaa gaacaatttg
     6061 aacaattaaa attttcatct gatgatatta tgatgttagt tcactgtggg tcaagaaatt
     6121 atggagaaga tattttaaaa aaattttatg ataaagatgg acttgaagtt gaaagtgaga
     6181 aagctataga ttatattaaa aaacatgata atgctttaat gtgggcagag cgtaatcgtg
     6241 aagctatcag taaaaaaatt atgcagtcaa taggtacatc tggagatgtt gaaacaatat
     6301 tctctataaa tcataatttt atagagaaaa gggaagataa atttatacat agaaaaggtg
     6361 ctgtttcaag tgaaagagga gctgtgatta taccaggttc aagaggcagt ctatcatata
     6421 tagttatgcc tactgaaaat acagagatat ctttatattc tctatcacat ggagcaggta
     6481 gaaaatggtc tcgttcagtt tgtaaatctc gccttaaaag taagtataat aaagatacta
     6541 taaaacaaac taaatttaaa agtcagatta tatgtaatga tgtaaattta ttatttcaag
     6601 aagctccaga ggcatataag aatatagagc agattattga aagtttgata gaatatgaac
     6661 ttattcaagt agtggcaact atgaagccat tgattactta taaaggataa tttttaaagt
     6721 taaaaaagat taaaattata ttataaatag ctaattttat ggaacattta aaagtgtttt
     6781 attattagct atttttttat tttgtagaga cgataattta tagagctaaa ttctttgata
     6841 tataatgatt acaaagttag actgtattgg aaaatgcaca tacatgaatt gatgacaata
     6901 taagtgattc ccaaatataa atgaaagtat aagcttattt tcactataaa aattttcact
     6961 gtacaaaaac attaatgaca ttatttttat tgacataaat atataattta tggaataata
     7021 ttagaagcac tgatataagt gtttaaataa gaatagaaaa ggagaagtgt tttatgaaaa
     7081 aaaatactaa cattaatgag tccggagtta aacttcttaa taaataagga gtttgatata
     7141 taataactcc ttatttatta agttgttcgc aaaataacta ttatcaaagt aaatgatagg
     7201 gtgatttaaa agacgataac ttataaataa aggagaaaaa ataatgaata gtaatacatt
     7261 tgaaagatta caactaaatg aagttaaaga attaataaaa atacattgtg taagttcact
     7321 aggaaaaaat ttaatagata aattaactcc tagtgggaat attggagtag taagaaggaa
     7381 attagcagaa aataaagaag ctagaaagat aattgaaaat agtaatcaca taccacttga
     7441 aggattgttt aatgcaggtt ctacaataga taaaattgaa aaaggaatga taatagaacc
     7501 tgttgaactt gtaaatattg aagatttttt aagaggttgt aggaagatga aagcttttat
     7561 gttagaaaaa gaattttatt cacctacatt aagttcttat gccttaaata taacagaatg
     7621 taaaagtata gaagatgaga taaattactg tataaaatct aataaagttg attctaatgc
     7681 tagtaaagaa ttaaaaaaga taagacgaaa tatagaaatt acagaaggaa agataaaaga
     7741 tagattaaat aaatttataa catcaactgt aaataaaaag tatatacaag agtttataat
     7801 aagtaaaaga aatgatagat atgtaatacc aattaaatct tcttataaaa atgaagttaa
     7861 tggtactata ttagatacat catcaaaagg aaatacggta tttattgaac ctattagtgt
     7921 ttcaaattta agtacagagt taactatgtt aaaagctgat gaaactattg aagaatacaa
     7981 aatattatct tatttaacag aacttatatt tgagaagata agtcagataa aattaaatat
     8041 agagatactt tcagaatacg atatggtatt tgcaaaagct aagtatagtc aaaaaataaa
     8101 aggaattact ccaaaaataa ataataatgg ctatataaaa ataattaaag gtaaacatcc
     8161 acttctaaca ggagatgcag tacctttaga ttttgaaata ggcaaaaatt atagaagtct
     8221 tataataaca ggtcctaatg caggaggaaa aactgtaacc cttaagactg ttggtttact
     8281 aactttaatg gttcaatgtg gatttgatat ttctgcaaaa gaaggtagtg aatttagtgt
     8341 atttgaaaaa gtttttgtag atatagggga taatcaaagt attgaaaatg ccttaagtac
     8401 tttttcatca catataaaaa atattgcaga gattatgagt ttatcaaata attctactct
     8461 tgttctgttt gatgaaatag gaagtggaac tgaacctaat gaaggagctg gtttagcaat
     8521 atcattatta gaagagtttt acaaaatggg atgtataact atagcatcaa ctcattatgg
     8581 agagataaaa aagtttgcta cattgcatcc agaatttgaa aatgctggta tgatgtttga
     8641 taaagaaacg ttagaaccat tgtataagtt aactattgga aaatctgaag atagtaatgc
     8701 tctttttatt tctaaaaaaa tgggaataaa aaatagagtt ttagatagag ctaaagaata
     8761 tgtaattgat agaaactaca atttagattt agttaaaaga agtaagctac aatctaatat
     8821 tgaagataaa aatagttctg aagtcatttt aaactataca gattataatg taggggataa
     8881 ggtaaaatta ctagatgaag atgattttgg aattgtatat aagcctatgg acaagtttaa
     8941 taatgtagaa gtattgttta atgataattt tatttcagta aatataaaaa gattacaact
     9001 tagcataaaa gcatcagaat tgtatccaga agggtatgat ttagatgtat tatttactag
     9061 ttttaaagaa agaaaattag aaaaagatat tcaaagaggt tcaaaaaaag ctcttaaaaa
     9121 gattcagaaa gaaattagag agaatagaaa ataaatataa aaagcacaaa agaaagcaat
     9181 ctaaagagaa aagtttatct ttagattgct ttcttattaa catatcttca taaatccaat
     9241 gactaacaag attaaaccac ttaaccaaga taagtttaaa tttacgtttt taacaaacat
     9301 atttcctagt aaacaaccaa gtaatactgc aattatattt gatattaaag aaaatagaat
     9361 aacttgtata taattcacac ctacaaggcc actaccaaaa ccgatagcta atccatctaa
     9421 acttaaagca agtgcaagtg taaatgcttc agcagattta agcactttag atttacttgt
     9481 atctaagaca tcaggttcaa tacttacatt aaaatttaat ttaaggtcta acatattaaa
     9541 attataattc tttgagtgta tagatttttt cttaaaataa gatttaaaaa aactttcaat
     9601 tagtctagca cttcctaata aaaataaaat aaagaaacat attaacattg ttatattttg
     9661 aggtaaaaag tctctaacta ttgtacctgc aaaaagcgat attgctaaaa tagctgaaca
     9721 aactacgctg ataattatat ttgaatataa gggaacttta actttattta cgccatatcc
     9781 aaagcttgtc acaaaggcat ctatacaaag agaagtaact aataaaattg attcaatcac
     9841 agtagattaa catcctttct cttaaatttt gtacttatat tagttatagt attgtaaaaa
     9901 tggataaatt gttacagata atccaataaa ttgttgataa aattccaaca tcatacaaca
     9961 cctaaataac aaaagaatca tatcatataa taatataaaa ccaatcataa taaagcaggt
    10021 gaaaataatg gaacaacata gtaaggttgg gaatttcggt gatttgttga aagttttatt
    10081 agatgaaaaa gaattatcaa tgggagaact aagtaaaaaa agtggaattg ataaatctac
    10141 tatatctcgt atagctaata ataaacaaaa gccaaatata aatcatttag aaaagatggc
    10201 aatacattta aatattaacc tagaagaact gttgaaagct tcaggatatg aatttagaaa
    10261 cagcaataat caaatattta atgcagatag tgattttagt aactttgatg atattcttgg
    10321 atttgcaaat ttaatcaatg ataagaactt taatggaaat attgaaaaag agttaagtaa
    10381 gtgtaagtta tatgtccaaa cagatgaagg taaaaaatta ctttttgata attttaataa
    10441 aaaaatagat tctattgaaa aacaaggtca atttacagat agattaagac aaatgtatgc
    10501 tgatttttgt acagatggac ttacaataaa gaaatattta ttgattggaa gtatgctact
    10561 ttactttgtt atatcaacag atgtgattcc agacttcgtg ttcccaatag gatttatgga
    10621 tgatttagta gcattaaata tagttacaaa attacttaaa aatgataagt aagttttaga
    10681 atcatattta atatataaat aaatcaaagt atataaaatt tattattttt tatatgaaac
    10741 aaattaaaag cacaatgaag tagattaaaa atttaaaact aagaatctaa tatatatttt
    10801 gtaactattt aagtatataa aataaaataa tatccaaact aatacaaaat agtaataatt
    10861 gttttgtact ggaggatata tatttatgaa ttgtaaatgg atatctaaaa tagatgagag
    10921 aaaaaaatgt catagagaag cagattcttc tggatattgt atctttcata aagaaaataa
    10981 gtctgatgaa gagatacaat tgatgatgga tactcttcat aaagaagaga taagtgaatt
    11041 taatggattt gtttttgaaa atgaatttaa tgcggaagaa atattaactt acaattataa
    11101 aatactggat ttttctgaat ccatatttaa acaaaaagca aattttaaaa aatatatttt
    11161 taaaaagaat attattttta actatacaga atttagagat aaagttttat ttaatggttg
    11221 tgtattctta gaaaactgtg attttaatag aaccatattt agtaaacact atataaatga
    11281 ccgtatattt gaaaaagtga aatttaaagg acctgatttg gtagttaata aagtagaaaa
    11341 ttttccacga atggatggaa taatttttag catgtgtaca aaatttgtgt taaagaatgt
    11401 tgaatatgga aaaagtgagt atgagcatgg caagataaat tatagaatag ctagaaatca
    11461 agcaacaaaa attggagaat atgagatgat aggattttac tattataagg aaagaattta
    11521 tagtagtaaa ataatgaaac gttctaatta tcctaccttc agtgattatt tagttgaaaa
    11581 attttttgac caaatagcac gttacacaac aggttatgga gaaaagccat ggaatatatt
    11641 attagttatt atagcaataa taagtgtttt tgctttatta tacttatttg taggcataga
    11701 aagttcaaat agcacacttg tagctttaga tataaataat ataggtgatt actcactaag
    11761 tgagattttc aaaatgtata tggatttatg gtattttagc atggctacat ttagtacagt
    11821 aggatatgga gatatggttg ctacaagttt gattgggaaa gcattggcag gtatagaagt
    11881 attttttgga gttacaatag gagcaatttg ggcatctgtg attattaaaa gaatgataag
    11941 gtaatatttg tgtaaatatg gataggtatt aattactaga tataaattgg actaaatagg
    12001 gaaaatttaa atataaatat acttaataat ccaagaaggt gaaaaatttg aaaaatgcac
    12061 taaaaaaaat tatatcatta gccatgattc tagtttttat aataccaaat tattcatatg
    12121 cagatgataa atctgtagac aagtattcaa aaacatctat acttatagac caagaaacag
    12181 gtagagtttt atatagtaaa gaacctgata tgaaggtacc attagctagt ttaagtaaga
    12241 tgatgacttt tttacttgca atagaagcta ttgaaaataa agaagtaaaa gaaactgata
    12301 aggtaaaaat agacaaatct attgcatcag taaaagggtc aagttataaa cttaaagatg
    12361 gtgaagaagt gccacttata gagcttatga gaggtcttat gattgtatca ggaaatgatg
    12421 cggcaattgc tatagcaaaa catgtttgta aaacgaaaga agcatttgta gatagaatga
    12481 ataaaaaagc aaaagaaata ggaatgagta atacacattt tctaaatcca aatggtcttc
    12541 caatatacga tttaagcaac cccaaaaaac cagcaaaaga aaatatctct actgcaaaag
    12601 atatagctat tcttggaaaa tacatgtttg accattatga aaaacaagta acagctataa
    12661 ctgatatgga gacatataca aattcagata gaagttttga aaaaagcaat acaaatgcat
    12721 tattaagaat tataccagaa gtagatggta taaaaactgg atatactggc aatgctggat
    12781 attgcttatc attttctatg gttgtaaata aaaatgataa aaatgagaaa aaacgtagag
    12841 taataggggt tgtattaggt actaaccata aaaataaaag aacctctgct tcattagctc
    12901 tactgaaata tggaaaagaa aatttcaata tgaaaaaagt aattgataaa gatagcttta
    12961 ttggtaagaa atatataaaa ggaacgaaag agttagaggt tacattaaaa gcaaaagatg
    13021 aactttatac aatattaaaa gatgatgaaa gtataaagtc agaagttaag attaaaaata
    13081 tagagtaccc tgttaaaaaa ggtgatacta tgggtattat aaaatattat actaattcag
    13141 gtgacttact tggaagtgta gatatagtaa gtaataatag tatagataac gtatcattaa
    13201 aaacaaaatt aaaaatgaaa tttgcaaatt aaattcaaaa aatggtataa aatacacaaa
    13261 aaaatataaa aatagaaaat atggaaaaaa tattgttatt tcttcacctc tgtgatatac
    13321 tactagaggt gaaaaaataa aataaatcac acacagcatt gggatcctta aaaggtgcct
    13381 attacatggt tttttaggga gaagatcaag ctggaggtat aaaaccagga ggtaagatat
    13441 gtcagtaata tcaatgaaac aattattaga agcaggtgtt cactttggtc accaaacaag
    13501 aagatggaac cctaaaatgg ctaagtatat attcacagag agaaatggaa tatatataat
    13561 cgacttacaa aaaacagtta aaaaagttga agaagcttac aagttcacta aagaagtagc
    13621 tgaaacagga aagccaatct tattcgtagg aactaaaaaa caagctcaag atgcgataaa
    13681 agatgaagct gaaagatgtg gaatgtactt tgttaatgaa agatggttag gtggaatgtt
    13741 aacaaaccac aaaactataa aaacaagaat aaataaatta agagaattag aaaaaatgga
    13801 agaagaaggc gtatttaatg ttcttcctaa aaaagaagtt ataaaattaa gagctgaaaa
    13861 agaaaaacta gaaaaatact taggtggaat aaaagacatg cctgaattac caggtgcaat
    13921 gttcgtagtt gacccaagaa aagaaaacat agctattcaa gaagctcata gattaggtat
    13981 accagtagtt ggtatagttg atactaactg tgacccagaa caattagact ttgctatacc
    14041 aggaaatgat gacgctataa gagccgtaaa attaataact ggtgctatgg caacagctgt
    14101 aattgaaggt agacaaggtg ctgaggaaga agtagctgaa gaccaagaat aataatttga
    14161 caattaaatg gtaagggtcg cccttaccat ttataatatg gaatagaata agtaggagga
    14221 aacttaaaaa atggctaata taactgctca aatggtaaaa gagttaagag aaagtacagg
    14281 cgcaggaatg atggactgca agaaagcttt acaagaagct gaaggaaaca tggaaaaagc
    14341 agtagattta ttaagagaaa aaggattatc aaaagctgct aaaaaagctg gtagagttgc
    14401 tgctgaaggt ctagttgcaa tagaaatgaa tgatgataat acagttgctt ctatggtaga
    14461 agttaactca gagacagatt tcgttgctaa aaacgaagac ttcaaagtgt tcgttaaaga
    14521 tgctgcttgt atggcattag ctactgataa agaagatata gcttctttat taggtgaaac
    14581 tcataaagaa ggaataactt tacaagaagt tttaaataat agagttgcaa aaataggtga
    14641 gaaattagac tttagaagat ttgctaaagt tgtaactaat ggacaagttg ctggatacat
    14701 tcacggtggt ggaaaaatag gtgttcttgt tgaaatggaa acagaagcta gagatgctaa
    14761 agttttagaa ttaggtaaag atgtagctat gcaagttgct gctatgaatc ctaagtatgt
    14821 ttcaagagat gaagtagatg ctgaatacat agcacatgaa acagaagtat taactcaaca
    14881 agctttaaat gaaggaaaac cagctaatat agttgaaaaa atggttaaag gaagattaga
    14941 aaaagaatta aaagaggttt gtttattaga gcaaactttc gttaaaaatc cagatataac
    15001 tgttaaacaa ttagttgctg atgttgctaa ggcagtaggt tctgatataa aagttgttaa
    15061 agttgtgaga ttcgaagttg gtgaaggtat acaaaagaga gaagaaaact ttgcagaaga
    15121 agttgctaag caacttaagt aataaccaaa gagagaacac gcaggtgttc tcttttttaa
    15181 aaaatagaaa aaattggata tgaaggattg aaataggagg tttctgatgg ataaaccaat
    15241 gtacaaaagg gtattgttaa aattaagtgg agaagcatta gcaggagaaa gaggttttgg
    15301 tataaataat gatgtagtta atgatattgc tattgctata aaaaaaatac aagaaatagg
    15361 tgttgaagta gctgtagtag taggtggagg aaacttctgg agaggcagaa ccagtgaagg
    15421 aatggacaga actactgctg actatatagg aatgttagct acagttatga atgcaatggc
    15481 gctacaggat gcactagaaa atatagatgt tgctacaaga gttcaaactg ctatagacat
    15541 gagacaaatt gcagaaccat atataagaag aagagcagtt agacacttag aaaaggaaag
    15601 agttgttata tttggtgcag gaacaggaaa cccttacttt acaacagata caactgctgc
    15661 tttacgtgct gctgagatgg aagcagaagt tatattgtta gctaaaaatg ttgatgcagt
    15721 atatgacaag gatccaaaag tacatgcaga tgctaagaaa tttacagaat taagttacat
    15781 ggaagttata cagaaagaat taaaagtaat ggattctaca gctacatctt tatgtatgga
    15841 taataaaatt ccaataaagg tatttgagct tacaacagag aatataataa gagcagtaaa
    15901 aggcgaaaat ataggaacta ctgtaaaata atgaagaatg aaggaggagt aaaattatga
    15961 aactggaaat acataagcaa ttagaagaga aaatgaatgg gacaattgat gctttaaagt
    16021 ttgaatttgg aactataaga gcaggaagag ctaatgcaca aatgctagac aaaataagag
    16081 ttgattatta tggaactcca actccaataa atcaaatagg tgctatatct gtaccagaac
    16141 caagaatatt aatgatatct ccttgggata aatctgctat gcatgaaata gaaaaagcga
    16201 tagctaactc agacttagga ttaaatccat caaatgatgg tgaggttata agactttctg
    16261 tacctgcttt aacagaagaa agaagaaaag aattagctaa aaaagctagt aaagctgcag
    16321 aagaatttaa agttagaata agaaatgaaa gaagagacgc caatgagaaa attaaaaaga
    16381 tggaaaaagg cggagaatta actgaagatg aattaaaaaa agcacaagat gaagttcaaa
    16441 aaatgactga taaatttata aaagaaatag atacattatt atctaaaaaa gaaaaggaca
    16501 taatggaggt ataatattga aaaaatatta taacctttta ggtcttcatg tagatgaggt
    16561 aaaacaatat tttgatgagc aaaatataaa ttataccgta aaatctattg aaggtaaaaa
    16621 ggataaagaa aaacttgtag ttcctagagt tataaaaata tctgagatag gtgatagtgt
    16681 agaactgata atcacatact tttctgactc cttagtttaa ggagtcagaa caatattgga
    16741 ggcaaaatat gaataacaac attatatatg atatagactt aaataacata cctactcata
    16801 ttgctattat aatggatgga aatggaagat gggcaaaagc tagatttctt ccaagaactg
    16861 caggacataa ggctggagta gaaactataa gagatatagt aaaagaatgc tcaaaattag
    16921 gtgttaagca cttaacatta tatgcatttt caacagaaaa ctggaaaaga ccgaaacttg
    16981 aagttgacac acttatgaac ttattatcta catacttaag aaatgaaata gctgaacttc
    17041 accaaaacaa tgtaaaagta actgctatag gagatataag tgctttacca aaaacttgta
    17101 taagagaatt aaactcagct aaggaactga caaaaaacaa tacaggagtt aacttaaatt
    17161 tagcattaaa ctacggaagt agagcagata taaaaaatgc tcttatagat atagtaaaaa
    17221 attgtgaaag tggtaaaata gatataaaca atattgatga agatattata aaaaactatt
    17281 taagcacaaa gtctatacca gatccagact tggtaataag aacaagtggt gaacaaagat
    17341 tgagtaactt tttactttgg gaagttgctt attctgagtt ttactttact gatatacatt
    17401 ggcctgattt taaaaaagaa gaactacaga aggctatata cgtatatcaa agaagggata
    17461 gaagatttgg aggacttaag taaggagatt attttatgct tacaagaatt attgcttcac
    17521 tagctctggt tccattattt ctatttgttg tatatggagg tattcctcta tatatagctg
    17581 agacagcaat agtttatatt gcattgcatg agttttataa ggcatttaaa ataaaagatg
    17641 tacatccaat attcattata ggatatttat tttctattta tttagctgta aaaaatattt
    17701 ttaatttacc attagaatat acttatgcag taatttttat tttattttta gcaagtataa
    17761 tttatatgtt gatgggcaaa aacaatgtta tagatgtttc gattacgttt ttaggagttt
    17821 tttatatagg agtattttta gattttataa ttataactat aaatggattt gaaaaaggta
    17881 gtatatatgt atggcttata tttgtgatat cttttatgac agatatattt gcatatttta
    17941 gtggttattt acttggaaaa cataagctaa ttcctaaagt tagtcctaaa aagactatag
    18001 aaggagctat tggaggaata ataggaagta cactttgttg tattttattt ggatatttat
    18061 ttggcattga tttgcttcaa ttagcgataa taggaagtat aggaagtgta atagctcaat
    18121 taggagattt atttgcatct tctataaaga gatatgtagg gataaaggat tatggaaaga
    18181 taatacctgg tcatgggggt atattagata gatttgatag tgttatatta gttgcacctt
    18241 ttgtatatag tgcgattaaa ttttttatta gataaagatt ggttggattt aaaagagcaa
    18301 tctataattg ctagtaatga aaatagctat gtaactttta aatgcagaga tattctgcag
    18361 ttattagtta catggctatt ttcattgttc aaaaatttag aagaccttgg ggaattgtat
    18421 aagaataggt attactttat aatagtatac tatatttgta gtgagtatat
//
LOCUS       DAMJVI010000079        17696 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.081, whole genome shotgun sequence.
ACCESSION   DAMJVI010000079 DAMJVI010000000
VERSION     DAMJVI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17696
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.081"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(11..775)
                     /locus_tag="PC545_002666"
     CDS             complement(11..775)
                     /locus_tag="PC545_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDF2457429.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSKPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            1049..1723
                     /gene="fsa"
                     /locus_tag="PC545_002667"
     CDS             1049..1723
                     /gene="fsa"
                     /locus_tag="PC545_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2457430.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSVTVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(1845..3239)
                     /locus_tag="PC545_002668"
     CDS             complement(1845..3239)
                     /locus_tag="PC545_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDF2457431.1"
                     /translation="MGIILKPKQFYMTVIATIILYFSLNYSFNIKFSICLSFLTILIW
                     AVDSVEKTFVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFTFVFKKELHSIKFISNENLEGNKLHFSYSNLKKEFNLLNKEQKHVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     KENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWKFIGLEI"
     gene            complement(3317..4663)
                     /locus_tag="PC545_002669"
     CDS             complement(3317..4663)
                     /locus_tag="PC545_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457432.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLKLMNTPESVFAQSKLYLTIIFSGILFSAGYNSVSAILRGLGDS
                     VTPLYFLIIATILNIVLDLTFIVVLRMGVEGVALATIMAQAVSFIISIIYLNKKHEVL
                     KFKIKGIVYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVTVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSKFFGTDGIWMGIPFGWTLGLIVTVIYYKKGHWKTKAIVNHRINE"
     regulatory      4913..5015
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5109..6485
                     /locus_tag="PC545_002670"
     CDS             5109..6485
                     /locus_tag="PC545_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2457433.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEIIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCVGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFI
                     NPEYAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPTNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSVVMIVLAIL
                     FIIKLWLL"
     gene            complement(6599..7744)
                     /locus_tag="PC545_002671"
     CDS             complement(6599..7744)
                     /locus_tag="PC545_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2457434.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAELSVT
                     SLALIGEVNIQKQIKNIKAVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKALAATNPKKAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MEKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRSGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELIDIIK"
     gene            7997..8704
                     /locus_tag="PC545_002672"
     CDS             7997..8704
                     /locus_tag="PC545_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457435.1"
                     /translation="MLKVINVSKSYKNKKVVSSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFEEGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            8751..10346
                     /locus_tag="PC545_002673"
     CDS             8751..10346
                     /locus_tag="PC545_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457436.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKADGREKQKIMGMMLSVVIIAG
                     LLIFYVTSLSLSLVDILKQINQLEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDL
                     LTSLPIKESTVLASKILMLVGANYLFSSICMLIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFLFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKVYPPIYYFVDALKTGSIVSFLAFIAISIIPFFMFVTLFAKKFKDINAK
                     MSEGYKSKNYEFKGQKTSRPSKALLKKEFKRYTSSFIYFLNSSAGLLFLIVGTILILL
                     FGADKVGELLKLNIDFNLIKIQLLGVFFFIIVMNCTTYCSISLEGKNLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMIVTMLVSLAYVIILGGIYVLLGISFLNIYIILASIL
                     TLILDFMIWRIISTKGVRIFKNL"
     gene            10449..11531
                     /locus_tag="PC545_002674"
     CDS             10449..11531
                     /locus_tag="PC545_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDF2457437.1"
                     /translation="MRKLSKREKDFIQYGFLLFLICITTYLVLTTLDIKLIPKIIRLV
                     NKTYIFLGILTIIVYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLMEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKIFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYVHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSNIIPKELLGYSVLLYSGFVYYFILIVSGLFTFG
                     THYTLNSWKKENKVNSNLDKVIEKLN"
     gene            11680..11841
                     /locus_tag="PC545_002675"
     CDS             11680..11841
                     /locus_tag="PC545_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HDF2457438.1"
                     /translation="MKQDKQNEKQSEISKIKQSNDNKLEIAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
     gene            12182..13450
                     /locus_tag="PC545_002676"
     CDS             12182..13450
                     /locus_tag="PC545_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDF2457439.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            13645..14433
                     /locus_tag="PC545_002677"
     CDS             13645..14433
                     /locus_tag="PC545_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2457440.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSKLNLDYIKEYEDYVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMEQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASREYRKAMSKVLLKRAIMEVI"
     gene            14433..14882
                     /locus_tag="PC545_002678"
     CDS             14433..14882
                     /locus_tag="PC545_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2457441.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKVGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRALDKEITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVLAMKDELENPTDDEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            14886..17150
                     /locus_tag="PC545_002679"
     CDS             14886..17150
                     /locus_tag="PC545_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2457442.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAIDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNKTMVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDVVIEETYYTQAQAHAMMETYRAYNYLDHMGRLVVV
                     SSTQIPFHVKRHLSRALEIPASKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKMDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKHLDNGKIRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLDTSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGATKLGVEKDEVK
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDTE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGRMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMEMNK"
     gene            17290..17601
                     /locus_tag="PC545_002680"
     CDS             17290..17601
                     /locus_tag="PC545_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457443.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKVRVFFKEK
                     QNQIEIDFAEEEISKLVENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGSRV
                     H"
BASE COUNT         6591 a         2077 c         2815 g         6213 t
ORIGIN      
        1 ataattaaac ttataaaaca tttacattaa attctgataa aatatctact atatcaggag
       61 taggcgtttt ttctgtaatt atagaactaa tatcttctaa atgagcaaac ttgaaatttc
      121 catcaaattt aaacttttta tcttccataa ctataaatac ttttttactt gaatttatta
      181 tagccttctt cgtaagacca tcttcaattt caaaagttgt aagtgaagag ttgaatacat
      241 ctactccaca acttcccata aaagtcttat caaacttata tttcgatata aactctatag
      301 ttggagtacc gacaaaaccg tctaaactga gatttaataa acctccagtg gaaattatat
      361 ttaaattatt tggttttgaa gtaactacat ttagtatatc cagcatattt gtaacaagtg
      421 ttactttttt attactttca gctaacaact tcgcaagcaa gatgttaatt gtagagatat
      481 ctaaaaatat agtttctcta tcttcaatta aattaaatgc tttttctgct attattttct
      541 ttgttggaat attaatttct tttctgtctt ttgtatcctg attctctagt gtttctctag
      601 ctagtacagc tcctccataa attcttctta ttgaagaaat cttttctata ttttttaaat
      661 cttttcttat acaatcttct gtaacattaa atttagaact gagctctttg acagtaactc
      721 taccttcttt ttttaggata tttaatatag cttggattct ttcttctgca aacattttct
      781 acctcttttc gcaatatagt attatagtaa atattataag ataaaataca atatatcaac
      841 tattttaaca tgaaatacaa aattaattta atataaaaag tatgtttttt ctaactatat
      901 atttagaatt tagtaaataa aaaaattata aaactaagaa ggaaaagtaa aacaataata
      961 aaaaataaaa aacaataagc aaaatgttga aaataatacc aaatcaatat ataataagaa
     1021 taaagaaaga ttaaaggggg atattttaat gcaactaatc ttagatacag gtaatgtgga
     1081 ggagattaag gagttatgta cttgtttacc aatagatggt gtaactacaa atccaagtat
     1141 tgtttcaaaa gaaaaaaaga attttaagca acttataaat gaaattggtg agattatagg
     1201 tgaggatatg cctatacatg ctcaagtgtt aagtacaaag tatgaagaaa tattagaaga
     1261 agcattatat ataagtagtt tgagaaaaaa tatatatgta aaaatacctg taacacaaga
     1321 tggtttaaga gctataaaag accttcatag aaaaggtgta aaaataactg ctactgcaat
     1381 attcactgct catcaaggtt ttttagctgc aaaggctgga gcaaattata ttgcacctta
     1441 tgtaaatagg cttgataata tatctggtga tggagtagct atggtttcag aacttataaa
     1501 aataatagat acttataaaa tggatacaaa agtacttgca gccagtttta aaaatgcaca
     1561 acaagttata gaattaatgc aacatggggt gcatagtgtt acagtaccat atgatatttg
     1621 taaatctatg atgaatcatc cattaactga ttggagtgta gataagttta tagaggattg
     1681 ggagaatact tttggaaaag gaagtaaaac aaataatatt tagtattatg gtaaaagagt
     1741 agttttgtcc aattttacaa gaacttatat aaaaagatga aaatatgaat tttcttttta
     1801 ataatttcgt tattatctta agttaattca tattttcatc ttttttagat ttctagtcct
     1861 ataaatttcc accaagggaa tgttatacat attattataa agtaggttaa aggaatcaaa
     1921 gttaaaccgt atttgaaaat aaccttgcta ttatacagat tttcaccatg accaaccatt
     1981 attgtagcat gatgaaatgg taatatgtac tgtatgctta ctataatgta gacaaataga
     2041 cataaagtaa cactattttc ctttagtata tgtaaacttc caagtaatgg tattactaca
     2101 gatgatgtag tgacagcact tccaagacat atattcaaga ttattgtgag agttatcaaa
     2161 aacaatattg aaataatttc tgaagagttt ggtataaact ttatcaaata atttcccatt
     2221 atatctacaa ctccagagta ctttaacact cctccaattg aaaatgctgc tataaaaaaa
     2281 ataaggacct tccaatttat ttcttttaaa gtactaaatc caactatttt tcttagtaat
     2341 aaaagaagta cacaaattga cattataatt aatgtgttta tgttatgtat gaactgagtt
     2401 aaaaacataa aaaacattat tcctaggcat aaaaatacgt gtttttgttc tttgtttagc
     2461 aaattaaatt ctttttttaa attagaatat gaaaaatgaa gtttattacc ctcaagattt
     2521 tcattagaaa taaattttat tgaatgcaat tcttttttaa agacaaaggt aaataaaaaa
     2581 taagttataa aacaagtaat tatagttgga acagacatat aaaaagccca ttgaccccaa
     2641 tttatattac aatttccaag ttctagtaca acataattta gaagagtgtc tccatttaca
     2701 aaaaacattg atgtaaaggt agatgctgta aatatactaa ataaaagtat agattttgaa
     2761 tcttcagtta tctgttgttc ttttaaaaac tccttgtaaa aagcagatac aagaataacc
     2821 cttggaaatg gttgtgggat aaaaaatatt aaaagtatac ctaaaatata cgataaaaga
     2881 atcatttttt taggggtatt tacagtattt aaaattaact tctcagaaat aatttttgcg
     2941 acacctgttt ttgtaactgc attagtaatt atgtatgcaa gaattattat gtaaaaattt
     3001 tctgtaaata aaaactgtaa aataaccttt attggtgcaa tgttaaatat aaacgctaga
     3061 attacaaaaa acaatgcaac aaaagttttt tcaactgagt ctacagccca tatcaagata
     3121 gttaaaaaag ataagcatat agaaaatttt atgttgaaac tgtaattaag agagaagtag
     3181 agaattatgg tagctataac agtcatataa aattgttttg gcttaagaat aatacccatt
     3241 tttatattca cctccataat ttattatatc ataatggagg tgaatacaat gaatgtgaat
     3301 acattttata aaaagattat tcattgattc tatgatttac aatagcttta gttttccaat
     3361 gtcctttctt atagtagata actgtaacaa taagacctaa agtccaacca aaaggaatac
     3421 ccatccaaat tccgtctgtt ccaaaaaatt tagaaagcat ataagaagct ggaagtcttc
     3481 caagccataa agatactatt gagcttataa gtgcaaacat ggaatcacca gcacctttca
     3541 ttgcacttga aagtacaaaa gaagttccaa taaataagaa gaatggtcct attataaata
     3601 agtaagaaga gccaatttta atgacatctt tatcagtatt gaatagagca attagatttt
     3661 ccctaaatag caaaattaaa gcaataactg taactgctaa aactaaggtc atttttatag
     3721 attctcgaat gcctttttta actctttcat tttctccagc acctatgttt tgagcaacaa
     3781 aagtagaaac agcagaacca agattcataa taggtaagct tataaaattt tctatcctaa
     3841 gaccagcacc aaaagctgcc atagcagatg taccataact attaactaaa aattgaagag
     3901 ccatatttcc aattgaaaac aacatttgtt gaactccact tggaagtcct aatctaagtc
     3961 catctttaaa tattttgtta tcatatacaa ttcccttgat tttaaattta agcacttcat
     4021 gttttttatt taaataaatt atacttatta taaatgatac tgcctgagcc attatagtgg
     4081 caagtgcaac gccctcaaca cccattctta gcactacaat aaaagttaag tctaaaacta
     4141 tatttagaat tgtggctatt atcagaaaat aaagtggagt tactgaatct ccaagtcctc
     4201 taagtattgc acttactgaa ttatatccag cagaaaataa tattccacta aatattatag
     4261 taagatacaa ttttgattga gcaaaaacgg attctggtgt attcataagc ttaagcattg
     4321 ggccaccaaa tactattcca agaatactta ttaatataga accaataaat aaaagtatat
     4381 atgtagtatc aatagtagct tttacctttt ttaaatctcc tgagccataa aattgtgaca
     4441 ctaaaataga aaatccaagg ctaacaccca taagcgctgc caccaataaa aacattatag
     4501 ggttagctcc agagactgcc gccattgctt cttttccaat aaatctacca actattatac
     4561 tgtcagctgt attgtatagt tgttgaaata aactaccaat aagcataggc atggcaaaaa
     4621 agaatatgga ctttccctca tgacctgtag ttaaatcttt caccttattc ctccaaataa
     4681 aaaaattatt aatttattat agtataatct taacataaac tttatatcca aactataaat
     4741 aaattgtatc aaaatatcat atattttttt aaaatgtaaa gaatataatt ataatttgaa
     4801 tttaataaat caatatttaa tataatcgaa tattaataca aaatcaatac aatatatacg
     4861 tatatattga caaaattaat tatataaatt aaaataatat tcgtaaagaa aaattcatgt
     4921 tttgtactgt ataacatata attttgacaa tatgggtcat aagtttctac cggaataccg
     4981 taaatattct gactatgtat actattattt agttaattaa atatttaatt aactgaatta
     5041 tgaaggatac ggctcagctt atcaactact gtgtcgtttt tttgtaaaat ttaataggag
     5101 gtgttgttat gcaaacaaca aacagtagta ctatgcagaa actttttcca attttgacta
     5161 ataaaaatgt cgacatgaag aaggaaataa ttgcaggagt aactactttt ttaactatgg
     5221 cttatataat agctgtaaat ccaaatattt tatctgagac aggcatgcct gcaggtgcac
     5281 ttgttacagg tacttgttta gcagcagcat ttggatgtat tttaatgggt gtagttgcta
     5341 accttccttt tgctttagca tcaggtatgg gtcttaatgc attttttgca tatacagtag
     5401 ttttacaaat gggtgttcct tgggaagtag ctcttacagc agtatttgta gaaggtatta
     5461 tttttatagt attatcagta agtggagttc gtgaggctgt tgtaaatgcg ataccaaaaa
     5521 atatgaaatt agctgttaca ggtggtatag gaatatttat tgcacttata ggtctagtta
     5581 acagtggaat tgttattgga gaccaagcca ctcttatcaa aatgggtaga tttacaccag
     5641 cagtaataat aacttgtgta ggattaataa ttattgctgt attagataaa aaaagagtta
     5701 aaggttctat actttttgga atagtagtga gttcattact tgcttgggca tttgctttta
     5761 taaatccaga gtatgcacaa aaattaggta tatatttacc tggtggatta tttaaattcg
     5821 aaagtttagc accaatagca ggtaaaatag atttaggata tgtactacat ccaacaaata
     5881 taggtggttt tttagtagta gtttgtacat ttttatttgt tgattttttt gatacagtag
     5941 gtacattagt tggtgtttgt tctaaagcaa acatgttaga tgaaaaaggg aatgttccta
     6001 atgtaggtag agccttactt acagatgcgg ttgcaactac agttggtgca ggacttggag
     6061 tatctacagt tacaacttat gttgagagtt caacaggagt tatcgcaggt ggaagaactg
     6121 gatggacagc aataacagtt ggaattttat tcttagctgc aatgtttttc tctcctgtat
     6181 ttatagcaat tccatcatgt gctacagcac cagctttaat atatgttgga tatttaatgc
     6241 ttggtacagt taaggatata gagtttgata atataactga aggtgttcca gcttttgtta
     6301 ctattgcttg tatggcatta acatatagta taggtgatgg tttaacttta ggtatattaa
     6361 cttatgtttt cgtaaatata ttttataata tatttggagc taaaaaggca gaagacaaaa
     6421 aacatgtttc tgtagtaatg attgttctgg caatattatt tataataaaa ctttggctac
     6481 tataataggt attatttaaa attttaattt aatataaaat aaagggctgt aatttattat
     6541 caattgtgat gataaactac agctcttttg tatttgttgt atttttgtat ttattgtatt
     6601 atttaataat gtctattagt tctccatttt gtaaagtttt aggtgtaatt actatattga
     6661 actctttttg caaatctttt ataatatcta attctttttt tgttattatt gagattgtgt
     6721 taccacttcg atttcctcta gcagttcgac cacatctatg caggtattca tttttacttt
     6781 ttggaaaatc caagttaaaa acatgactta catccacaat atctagtcca cgagcagata
     6841 aatcagttgt aattaaaatc tttgctttac caagtttaaa tttatttatt gcattctttc
     6901 tatcttcttt ttccatattt ccaaaaattc ctattgcttt gtaattatga taatttagtt
     6961 ttgatactag gacttctata tttttttcat tatttacaaa tacaatagct ttttttggat
     7021 ttgtagctgc taatgccttt ctaagaaaag taaatttatc ccttatctca cctaataaat
     7081 atgagtgatt gatgttagga tttatttgtg atttttcctt tgtttttatt atttcaaact
     7141 ccttcataag tttatcacaa atttttatag tgctatcagt aagacttgca gagcatccta
     7201 taatttgtct atctcttaac gttgttctta ttatgtcttc tatacaagtt atattttttc
     7261 cattcaatag attgtccact tcatctaaga caatagtttt tatattatgt gattttagtt
     7321 tcttttgttt tatcaagtct aaaactcttc cacaagagcc aattactatg tgtggtttta
     7381 ctgcttttat attttttatt tgtttttgta tattgacctc cccaattaaa gctaaagaag
     7441 taacggagag ttctgcattt ttagccaata attcaacctg gtttgtaatc tgcattacta
     7501 attcatgcgt aggtgcaagt ataagagctt gtgtttctct ttttgatgta tcaatttttt
     7561 caaatatagg taaaaggtac gctagtgttt ttcctgttcc agtctgtgaa ttaatcaata
     7621 aatccttatt ttgaattata ttccctataa caagtgactg aacttctgtt ggagaagtta
     7681 tgtcttgttt tttgagtcca tctattagag tggaacttat ttttaattgt tcaaatgtat
     7741 tcatatttta ctccttatat tttaataatt atagtcaact attttttatt atacattgtt
     7801 tattcaaatc gaactatata ttcttttata attatgtgta atttgaaagt aaatatcaat
     7861 tatatttata cctttatatt tctgatacct ttgcatttta tagatttcta aaataaacaa
     7921 ataaaagttg taattgtttg atatttgtta tatattgtat ataacaaata tcaaaactat
     7981 aatatggagg gaaattatgt taaaagtaat caatgtatct aaatcatata aaaataaaaa
     8041 agtagtaagt agtgtctcat ttgatgtaaa tgatggagag ctttttgggt ttataggaca
     8101 taatggagca ggtaaaacta ctactattaa agcaattgtt ggaatacacg attttgaaga
     8161 gggagaagta ctaataaatt ctaaatctat aaaaaaacag cctgttgaat gtaaaaagga
     8221 aatggcatat atacctgata atccagattt atacgagtca ttaactggta tacaatacct
     8281 taattttata gctgacatat ttgaagttgc caaagataaa agagaagagt taataaaata
     8341 ttattcaaat aagtttgaac tcaataaagc attaggagat ttaatatctt catattcgca
     8401 tggtatgaaa caaaaattag ctataatttc ggctttgata cacagtccaa aaatcttaat
     8461 tcttgatgaa ccttttgtag gtcttgaccc aaaagcttcc tttatattga aggaaattat
     8521 gagggagttt tgtgacaaag gtggttgtat atttttctca acacacgttt tagaagttgc
     8581 tgaaaaaatt tgtgataaga ttgccataat aaaagatgga aatataattg cttatggaac
     8641 tacagaagaa gttaagggaa gtaattcact tgaaagtatc tttatggaat taatagagaa
     8701 ataaaatgtt ttttatacaa tatagtgtct aaagtgagga atgataaaat atgagcaatc
     8761 taagtatatt aataaaaact aatataataa atgaatttaa attaaatgcg ttaaaaaaag
     8821 ctgatggtag agaaaaacaa aaaataatgg gaatgatgct ttcagttgtt ataatagcgg
     8881 gtctattaat tttttatgta acaagcttaa gtctctcttt agttgatatc ttaaagcaaa
     8941 taaatcaact ggaaatgcta ctgatatttg gttttggatt atcaacatta acaactattg
     9001 ttacatcatt atataaaacg tcatcatatc tatttcaagc aaaagattta gaccttttga
     9061 ctagtttacc tataaaagaa tctactgtac tagcaagtaa gatattaatg ttggttggag
     9121 caaactattt attttcttca atatgtatgc ttatacctgc agtggtttac tttttaaatt
     9181 ctgatgtaag tataacttat ttcccttatt tgatattgct atttttagct ttaccattat
     9241 taccgatagt tgcttcatca ataatttttc tatttatagg gcagatatca tcaagaataa
     9301 aatacaaaaa tttagtatta atcataggta gcattatatt gatgttgctt tataccctac
     9361 ttatgtcaag acttaattct atgtcagttg aaatgctaaa gaatagtgtg tctatttcag
     9421 agacgattag aaaagtatat cctcctatat attattttgt tgatgctctt aaaacaggaa
     9481 gtatagtttc atttttagca tttatagcca tctcaataat accatttttt atgtttgtaa
     9541 ctctgtttgc aaaaaaattt aaagatataa atgctaagat gagtgaagga tataagtcaa
     9601 aaaattatga atttaagggt caaaaaactt caagaccttc aaaagcttta ttaaaaaaag
     9661 aatttaagag atatacttct tcgtttatat actttttaaa ttcatctgca ggtttgttat
     9721 ttctaatagt aggtacaatt ttaatattat tatttggagc agataaagtg ggtgagttac
     9781 tgaaattgaa tatagatttt aatcttataa aaatacaatt attgggagta ttcttcttta
     9841 taatagtaat gaattgtaca acatactgtt caatttctct tgaaggaaaa aacttatgga
     9901 tactgaaatc ttctccaata gatgaaagag aaatattctg gtcaaaaata ttgatgaatt
     9961 ttttattgaa tgcaccacta tctattttat gtctaatatt agtatctata agattagact
    10021 ttgaaattca gtttattatt gtatcaatct gtttgattgt aagtttttca atgtttgtag
    10081 cattgatggg attattatca aatttattat acccaaacct tgattggaaa aatgaggtgg
    10141 ctgtagtaaa aagaagtgca agtatgatag ttacaatgtt agtatcatta gcttatgtaa
    10201 ttatacttgg aggtatatat gtattacttg gaatttcgtt tttaaatata tatattattt
    10261 tggcaagtat acttactttg atattagatt ttatgatatg gagaattatt tctactaaag
    10321 gagtaagaat atttaaaaat ttataggtgt atttatgtca aataaagact tatctttttt
    10381 agtcttgcaa ataataggtt aagtagtata ctatgtatag tgatatgaaa aaatttagga
    10441 gaaaataaat gagaaaatta tccaaaagag aaaaagattt tattcaatat ggatttttat
    10501 tatttttgat atgtattact acttatttag tattaactac tttagatata aaactaatac
    10561 ctaaaattat aaggctagta aataagactt atatatttct tggcatattg acaattatag
    10621 tatatatgat aattgaatct attgtaactc atttgataat aaatgcagtt caaaaaacaa
    10681 aagtaaaatt tattggtatt aaaatggcta caatgggatt ttactataac ttagttactc
    10741 catttgcatc tgggagtcaa ccaatgcaaa tatatgcact tacaaaatat gatgtaagcc
    10801 ttagtaaatc agtggctata gttactaata aaactgtagt atttcaaagt acagttactg
    10861 tattttgtgg aattttaatt ttgctaaata agtcattact gatggagcaa gtacattctg
    10921 tgaaagtctt gataactttt ggaatgatta tgaatgtaag tatgttgtta ggtggactac
    10981 taatagtatt tagtccaaaa caagtaaaaa tgatagcaga agttttgatt aattttttat
    11041 ctaaatttaa aacatttaaa tttttagatt caaaacgtga tagaataaat cattatattg
    11101 atgattataa ttattctata aaaatattta ttaaaaatgt aagaatctta gttttaagta
    11161 tattacttac tgtgttacaa ttaatcgcat atttcagtat agtttattgt gtgtataaag
    11221 cctcaaattt aagaggagtt tcatatgtac atataatgac attacaggtc tttttatata
    11281 tggctatatc accaattcca acaccaggaa atgttggagc taatgaggtt acatttttta
    11341 ctatgttctc caatataatt ccaaaagaat tattggggta ctctgtactc ttatatagtg
    11401 ggtttgtata ctattttata ttaatagttt caggtttatt tacatttgga acgcattata
    11461 cattaaattc ttggaaaaag gaaaataaag taaattctaa tttagataag gttattgaaa
    11521 aattaaatta ataaaataat tagggaaatt ctatatataa atatggtgtt gaataagcta
    11581 tattatattg agatatttcc cttttttgat taaaattata gaaatttgaa ataataagta
    11641 ataaacagaa aaagtgatat tgttttgaaa ggagaaacta tgaaacaaga taaacaaaat
    11701 gaaaaacaaa gtgagatttc taagataaaa caaagcaatg ataataaact tgaaatagct
    11761 caagaattac atgtaagtaa aaatataggg aatatgcttg gatatagaat agataagaaa
    11821 aaaatgaata gaccaagtta atgaaaatgt tgaaaatggt acattttaaa aaatatatat
    11881 aatgaaatgt tttattgtta aatttaaact tattttaatg ggggaaaaag tcgaaaaata
    11941 tctaaaaaga tatggatata aaaaatatta atatatatat actataaact tgacaaaatc
    12001 aaccttttaa taactagcaa aaaatagcaa aattatataa gcgttattta attgtcaaaa
    12061 atgaaagcta aattgcatgt cctaaaaaaa atgattgata acatttattt gtgtgctata
    12121 attatatagt aaaacaataa gtatattgca taatgtatgc aaatcgggag gagagacgga
    12181 catggaagca tctatacaca caattgccag caataattta ttgataattt ttgctattgt
    12241 aagtataact ggaataattt gtagtaagct tagtgaaata ataaaggttc cagatgtagt
    12301 gttatattta cttgtaggta ttttaatagg accatcattt ttaaaattta tagatattcg
    12361 tggatttcaa atagaaaatc aactaatact tacatttggt tctgcattta ttttatattt
    12421 aggtggaaaa gaaataagtt taaaagtatt gagaaatgtg aagatatctg tattcttatt
    12481 agctacttta ggagttgtga tatctgcttt tataatgcaa caagtaatag gatttacttt
    12541 tgggataagt gcaatgactg ctttactagc aggaactata atagcatcaa cagacccagc
    12601 cactcttgta cctatattta atcaggtaaa gataaaggat agggtaaaac aaactgtaat
    12661 aagtgaatca gcttttaatg atgcaacagg agctatacta acatcagcag tgttggctgt
    12721 aattttgtca aataaatttt cattaggtga aaatatttat gaactgggga caatggttat
    12781 tgtcggagtt ttagttggtt taattacagg aatactgctt ttgaaattag ttaatgataa
    12841 accttatgga atatttaaag attttgcacc aataatatct gttatttcag ttgtagttgc
    12901 atatgaatta tctacaaaat taggtggaag tggatatatg tcttgcttta ttgtaggtat
    12961 aataactggt aacaagaaaa actttaaaat ttggttaagt caaaaatctt atgatgcaga
    13021 tttttatgtt gctgaaactt taggtactat ttgtcgtatg gcaatattta taatattggg
    13081 tagccaagtt aatttggctc agttaagtaa atattttatg ccatctgtaa ttacagtatt
    13141 agtattaatg tttatagcaa gaccactttg cgttttagtt tgtacacttg tagacaggga
    13201 agcaaaatgg agtaaaagtg aaatattatt tatgatgtgg gtaagggaaa caggcgtaat
    13261 accagcagca ctttgtggta ttatttcagc aatgaaagtt cctggatacg aagtgatttc
    13321 atctgtagta tttatgacta tactaattac attagtaata caaggaagta ctacaaaatt
    13381 agtagctaaa aaacttggat tattagaaga agaagtgata aatataagtg aaaaagtatc
    13441 aactttttaa agttttgctt taaaatagta cctgttaatg gctattttaa tattgtctaa
    13501 tgatgattat tgaaatagtc atttttttat gtataagtaa tttacagaat ttacatatta
    13561 gacatattta ttatcaaaat ttgttgaagc tatgatataa ttagttgtat ttaaaataag
    13621 aatagcaaat aagggaggaa atttatgttt acggttatgg atattgtaca gccagataca
    13681 gtagaagaag cgtactcaat tttaaataaa agaaaaacta accaagtaat tggtggaagt
    13741 gcttttttaa gaatggggaa aaaaagaata ggaacaggaa tagaattatc taaattaaat
    13801 ttagattata taaaagagta tgaagattac gttgagattg gttctatgac tacttttaga
    13861 acattagaaa caagttccat aataaaaaat aattttggaa gaataataga agattcagtt
    13921 aaaaatataa taggagttca gtttagaaat gtagtaacag ttggtgcaac agtattttct
    13981 aaatatggtt tttctgactt gatagtagca ttactttcat tagatacaga agtagaatta
    14041 tataatattg ggagaattag tcttgaagag tttctaaata gagattatga gaaagattta
    14101 cttattaaga tatacataaa gaagacaaat aagaatgctt cttataaatc acttagaaat
    14161 gcaaagagtg attatccaat tctaaatgta tctgtatcaa aacatatgga acaatttaag
    14221 ttatgtgtag gagcaagacc gcagaaagct acgattgcaa aacaagctag tgaatttcta
    14281 tctaacaatg aaatcaatga aattaatata gataaagcag tagaaatagc atcagaagaa
    14341 ctaacttttg gttctaacat gagagcatct agggagtata gaaaagctat gagtaaagta
    14401 cttttaaaaa gagctataat ggaggttatt taatgttagt aaagattaat ataaatggga
    14461 agaaaaaaag tgttgatata gaaccagaag aatatctggt tgatacttta agaaaagttg
    14521 gtaatttaag tgtgaaaaga ggttgtgaca caggctgttg tggtttatgt acagttttaa
    14581 ttgacaagaa acctacatta tcctgtgcaa cactgacatt gagagcttta gataaagaaa
    14641 taactacaat tgaaggttta gaaaatgaag ttaaagaatt tagtgaagtt ttggtaaaag
    14701 aaggagctga gcaatgtggt ttctgttctc ctggctttat acttactgta cttgcaatga
    14761 aggatgagct tgaaaatcct acagatgatg aaataaagca ttatcttaca ggaaatctat
    14821 gtagatgtac agggtatatg ggacagttaa gagctatcaa aacttatctg gaggtaaaat
    14881 agaacatgaa tatagttgga aaaggaatca agaaaataga tggaatgagt atcacaacag
    14941 gaaagcctgt atatacagat gatttatcag caaaagatgc tttggtggta aagatactta
    15001 gaagtcctca cgcttatgca aaaataaaag atatagatac atcaaaggca gaaatggttg
    15061 atggtgttga atgtgtttta acatataaag atgtaccaaa atctcgattt acacttgctg
    15121 gtcaaacata tccagagcca tctccatatg atagattaat tcttgaagat gttgtaagat
    15181 atgtaggtga cgaagtagct atagtggcag ctatagatga aaaaactgca attaaagcaa
    15241 tgaatcttat caaagtaaag tatgaagtac ttacacctgt tttggatttt gaagaggcaa
    15301 taggaaataa aactatggta catgatgaag atagacattg taactttgat ataggtatgc
    15361 aaagagaaaa aaatatagta tcccatcatg attatacaaa aggtgatgta gaaaaagtat
    15421 taagtaattg tgatgtagtg attgaagaaa cttattatac tcaagctcaa gcacatgcaa
    15481 tgatggagac gtatagggca tacaattatt tagaccatat gggaagattg gttgtagtaa
    15541 gttcaactca aataccattt catgtaaaaa gacatttatc tcgtgcacta gaaataccag
    15601 ctagcaaaat tagggttata aaacctagaa ttggtggcgg atttggtggt aaacaaactg
    15661 catgtgttga gattttttct gcaattgtta ctttaaaaac agggaaacca gctaaaataa
    15721 tttatgatag aaaagaaact tttaattgtt caacaagtag acatgcaatg agattaaaag
    15781 ttagactagg tgctacaaat gatggcataa taaaggcaat agatataaaa gcactttctg
    15841 atacaggagc atatggagag catgcttcta caactattgg tttggttgga gaaaaaactt
    15901 taccaatgta taataaagtt gaagcaacaa gatttattgc agatgtggta tatactaata
    15961 agatgccagc aggagcactt agagggtatg gagcatcaca aggatgtttt gctgttgaat
    16021 caactataaa tatgcttgct aataaattaa aaatggaccc aacagattta aggttaaaaa
    16081 atgtggtaac agaaggtgaa actacatttg catatggtaa aactttaaat agtgtcgatt
    16141 taataaaatg tataaataaa ggcaaagaac ttattggatg gaatgaaaaa tatccttgta
    16201 aacatcttga taatggaaaa attagaagtg tgggtatggc aattactatg caaggttctg
    16261 gtatacaagg tcttgataag tcttcagctg aaataaaatt gaatgactgt ggagattata
    16321 ctcttatgat aggttctact gatatgggta caggaagtga tacgatttta gctcaaatgg
    16381 ctgctgaggt tcttgataca agcattgaaa atataacagt tatatcagct gatactgact
    16441 tagttccata tgacccaggt tcatatgctt caagtactac ttatgtaaca ggtatggcag
    16501 tagttaaggc agctcaagac ttaagagaaa aaataatttc tgctggagca actaaactag
    16561 gtgttgagaa ggatgaagta aaatttgatg gtataaaatt aactagtaaa aaaggtagca
    16621 ctttattatt cgatatagca gttgattcta caattggtcc agatactaat caattaatag
    16681 gtcttgcttc tcatggtagc ccagtatctc ctcctccttt tatagcaggt tttgcagaaa
    16741 ctgaaattga cacagaaact ggaaaagttg atttagtaaa ttatgtagct gtagtagatt
    16801 gtggaacggt tataaataaa aatctagcta agatacaagt tgaaggtgga attgttcaag
    16861 ggattggaat ggcattattt gaagaagtta gatacacaga aaaaggaaga atggacacaa
    16921 atacatttat gcaatacaag attccagcta gatgtgatat tggaaatgtt aaagtagatt
    16981 ttgttgaaac ttatgaacca actggaccat ttggtgctaa atctgttgga gaagttgtta
    17041 taaatacacc tttaccagca atacaagaag ctgtgtttaa tggaattggt gtaagaataa
    17101 atacactccc tattacacct gaaaaagttt ttatggaaat gaataaataa ttatttatga
    17161 aaatttagac tagttttaca tgttaaacaa ccagttatgt catacctgtt gaaagatatt
    17221 taataagtag ttattataat attatgaatt ttacttataa aatacaaaat taaataaggg
    17281 gatgatgttt tggctggtaa tctaaataat atgagagcag taaataattt tagaggagat
    17341 aagaacattt tagaatgttt agtcagcttt gagggtcgtt caataagtca gagaaaagta
    17401 agggtatttt ttaaagaaaa acaaaatcaa atagaaattg attttgcaga agaggaaatt
    17461 tctaaattgg ttgaaaatgt tgttttaaat acatcatatc aagaaatgtt atatgatgaa
    17521 atagagaaac aactggaaat tgattgtata ggtacttgga tgatattatc taaattaaaa
    17581 gatggtagta gagttcactg attttaaaat aaattattag agcctaattt taaggctctt
    17641 ttttgctgtt tttaattaac aatagaaaac ttatctttag tttgtatata atttta
//
LOCUS       DAMJVI010000080        17346 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.082, whole genome shotgun sequence.
ACCESSION   DAMJVI010000080 DAMJVI010000000
VERSION     DAMJVI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.082"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            82..828
                     /locus_tag="PC545_002681"
     CDS             82..828
                     /locus_tag="PC545_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDF2457444.1"
                     /translation="MKIENLSIDDIKRGYIFNDDLNCYSCIKCTKTYEVGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQLLNSDSKYNTLTENQKQLFQLFISDLPDKEIAKQVG
                     VSTSTIRHQKFIFREKAKQAKFYLALYESVFEDKSNKSSTIIPIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKKYSEKEVNQILES
                     IYDDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            889..1563
                     /locus_tag="PC545_002682"
     CDS             889..1563
                     /locus_tag="PC545_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2457445.1"
                     /translation="MNDELKSILYNKGVDIVRFVDISEFPINQTHGFSKAILFCIGLS
                     KKFITDIYNNLPTDSDEFLEKEEKVEELADWISKYIQNKGYRAYSQSEKNNLGHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RYPLIDSKCMDCNVCVENCPAKAIHGNEWTLPGKRESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            1931..3277
                     /locus_tag="PC545_002683"
     CDS             1931..3277
                     /locus_tag="PC545_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2457446.1"
                     /translation="MEIETKTPDWINVLELNSKIDITGKYDVSNMIQDIVSDKSLEGS
                     IIYFPKGNYLFEKGIKISQQITLQGDSYYGGDNQVSNLDKKQSIIGTTNFITRGVSNM
                     SIIALTGTSQCIKNINFYYDSHDIEKIPPKNVSAITEYGETQGLSHFEHLFISGFSGI
                     GIEIPYYSTGNDITVSSCGLGMRLGEKSMLSSSKIYECKNGMEITTGVSLNNVRIEEI
                     KEIGINNKGFGFNLIMNLMVDQCGYYGFMFEKMCYSQITARITRCGQYYKSVDYNAYE
                     NMEDRKEEAYSIFYGDILENCNIVLMNSNIDTLENDSLDEHKIYVIKANETNNVMLTC
                     YAEADNFIQCAKGNLLLENGVNTYKFYNGEICSVGGVQISDIDEKDLIKIIENTLYID
                     DKGKYLMVPRLKKNTVIYSVLDSTEEINKLHGGIWEQIDVKVLAGESMYYYKKIKD"
     gene            3304..4704
                     /locus_tag="PC545_002684"
     CDS             3304..4704
                     /locus_tag="PC545_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2457447.1"
                     /translation="MEESIIIKDISGWINVLDYGICNDETKDVSFKLQEIMDAVPEDS
                     IIYFPKGKYLFESGVKIKKRLTLCGDSYILANVPKNTAGVTQFTFKGKKNPKSENIAI
                     ITAKSVRHCIKNICFYSDSCDRDMTSNHEPPTDGKPRFHHNVIINYRNISAVICEDKQ
                     GEPGHYENLSFSGFSGIALDMPNNSTANDITIFTSGLGISTGENSTIANSKVWGCVDG
                     MIISTGTFINGMRVEEIKKVGIKNIGRGLNFITNITIDQCGYCGVWFDSISNVQISGN
                     ITRCGQYYYDVSYDNYLMLDNRVKEAYSLFYGNLLESSNIVLSNTNEDNWEDFKEDKH
                     KVYLIEALKTKNVLLTCNIDATDWIVKSEKGNLTYNNMRQTVIFYDGKLSNINGIGIS
                     DEDYGDKVKIKKGNIYINSNDKDIKMHKPEVGDIMSTIIKDKSILSAHYGGTWEKIGE
                     KIEFGITVHYYKLIEK"
     gene            4914..6350
                     /locus_tag="PC545_002685"
     CDS             4914..6350
                     /locus_tag="PC545_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 55 protein"
                     /protein_id="HDF2457448.1"
                     /translation="MEGRTGIKDGRWINVVERGIKNDGSYNVGPALQDIIDEIETDFC
                     VLYFPKGKYLFTSGVKINKEIVLQGDSNSSTGTTQFITRGVENMSIITLTGKKQCIKN
                     INFYSDSCEIQVNKEPPAKGNPKYHYEMIINKNEKNVEMNNVSAILYDNSKNMIKGLG
                     HYENIYISGFSGTGIRIPYYSIVNDITVSSCGLGIDTGIDTIISNSRISKCQNGMRIR
                     TGTSINNVRIEKIQKVGLISSQVKGYEGYGSYKINNITIDQCGYCGFSFDSMKDSYFS
                     GIIRRCGQYYYNTDYDTYLSIKDRVEEAYSLFYGNYFTECNIDLVSDNKDNWDDGLEY
                     KHKVYVFKTLQIPKLTLRCNIDAYDYIVKSVHGGGNLMLKNNFNNYIFIDSSSPEVIN
                     GINFNYQKNGNLLRLNNGKLSYKNGKEETAIFRPNYGLIVASTDSECEQIEATYGEKW
                     EKLGSEVIGKETVYYYKHHEPEKTNQIQ"
     gene            6694..7638
                     /locus_tag="PC545_002686"
     CDS             6694..7638
                     /locus_tag="PC545_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HDF2457449.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLEEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMLYDLNKLRDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELSRKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGIILEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRDDAKISIRDMKTIKVDNNKQISIFD"
     gene            7807..8739
                     /locus_tag="PC545_002687"
     CDS             7807..8739
                     /locus_tag="PC545_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDF2457450.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGFIGFILY
                     AIYIVKIILLRKGVKEQLENPLISSTFPTITMATMIFATYIKPLSLELSIMIWSIGIV
                     GHLVLIVLFSKKFLVKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLMNGYLTKSGNFISWISY
                     ASKLEEIIATVIILYVLVLFIIYLSKSETTSKAK"
     gene            8844..9311
                     /locus_tag="PC545_002688"
     CDS             8844..9311
                     /locus_tag="PC545_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HDF2457451.1"
                     /translation="MKTKEEYLKEVRNISEEYFRKGEFFCSEAVLQTINDALGQPLSP
                     EITKLASGFPIGLGKAQCLCGAISGGEMALGIVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRAITKKWDGDNFMSPERKEHCIKITGQVTEWVADKLIEDEKLNIK"
     gene            complement(9555..10409)
                     /locus_tag="PC545_002689"
     CDS             complement(9555..10409)
                     /locus_tag="PC545_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDF2457452.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMISGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVYRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKEKKNKKEWLAIFIGIILIMSG
                     VFMLSIIK"
     gene            10575..12539
                     /locus_tag="PC545_002690"
     CDS             10575..12539
                     /locus_tag="PC545_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HDF2457453.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIADDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLIRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIDILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKGEYIEESLYTGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVASDQHRDTNELKNTSTNPCETSTNNISNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDDECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSSCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAINHITDNLIKKGFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            12539..13000
                     /locus_tag="PC545_002691"
     CDS             12539..13000
                     /locus_tag="PC545_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2457454.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHKIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            13003..13323
                     /locus_tag="PC545_002692"
     CDS             13003..13323
                     /locus_tag="PC545_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDF2457455.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            13339..14439
                     /locus_tag="PC545_002693"
     CDS             13339..14439
                     /locus_tag="PC545_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDF2457456.1"
                     /translation="MLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGSFYNIP
                     DIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPGLVGGM
                     IAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLITVVLIN
                     YVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTSLALLT
                     SGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSEGAIPF
                     AVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSIAVGTV
                     IVAGLVIVLRGNPVIEDDEFEDDDFDEAEWES"
     gene            14559..17159
                     /locus_tag="PC545_002694"
     CDS             14559..17159
                     /locus_tag="PC545_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDF2457457.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLHKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSLKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKIKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARIH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLINTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISEGLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDATIIEETYVKPRENVLEETPAGNIFIEEPGYYILKVR
                     INIQLPALGYKVVSFELSDKEMVSLDKSNDTFISNDNYKIIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDKVYTEKALGYEKMVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVSIVLSKENPLMDVKLCVDNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIEDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQMLKELEFSFAIYMDSKS
                     FDELEIANLTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIVNKTVSRELEIISLPENY
                     LISACYPSYYSKDKFIIRVENPSSNRMILDEQIFKNRNGKIVNAIEDVEEEQVYEIAP
                     FDVISILLDL"
BASE COUNT         6894 a         1785 c         2999 g         5668 t
ORIGIN      
        1 aaataaaaat gttgattaca ttaataaact ttaatataat atatttatga aaaatgtaaa
       61 ctgtgaaatg gaggtagtaa catgaaaatt gaaaatcttt ctattgatga tattaaacga
      121 ggctatatat ttaatgatga tttaaattgt tatagttgca ttaaatgtac aaaaacatat
      181 gaggtaggag aaatatataa aataggtaat agattctttg aagcttcaaa agctattgaa
      241 cttcatatgg aattagaaca tagtgattat ttagagcaat tgcttaatag tgattcaaag
      301 tacaacacat tgactgaaaa tcaaaaacaa ttatttcaat tatttattag tgatttacca
      361 gataaggaga tagcaaaaca ggttggagta tccacatcta ctatacgcca tcaaaaattt
      421 attttcagag aaaaagcaaa acaggctaag ttctatttag cactatatga atctgtattt
      481 gaagataaat ctaataaaag tagtactata ataccaatcc atgaacatgc tcgaatggta
      541 gatgaaagat atgtagtaac tgaagaagag agaaaacaca ttttggatac atcatttgaa
      601 agtatgaatc ctttaaaatt aaaagtattt tcttcaaaag aaaagaaaaa ggtcgtaatt
      661 ttatctaaga ttttggaaca atttgaacgt gggaagaaat attcagaaaa ggaagttaat
      721 cagattttag aatctatata tgatgatttt gcaacaatta gaagatatct aattgagtat
      781 ggttttatga gtagaaataa agaatgtaca aagtattggt taacataatt aaaataataa
      841 attttactaa tgtgtaatgt tatttttatc tagggggagg agtcaaatat gaatgatgaa
      901 ttaaaatcta ttttatacaa taaaggtgta gacattgttc gctttgttga tatatctgaa
      961 tttccaataa atcaaacgca cggtttttct aaagcaattt tattttgtat agggttatca
     1021 aaaaaattta ttacagatat atacaataac ttacctacag atagtgatga gtttttggaa
     1081 aaagaggaga aagtagaaga attagctgac tggatttcaa aatatattca aaataaagga
     1141 tatagagcgt attctcaatc ggagaaaaac aatctaggac atggatattt tgaaaaagcc
     1201 tatattaatc cagaaatgca atcagggatt agtcctttac cacataagac aatagcaaat
     1261 attagtggca ttggatttat gggaaaaaat aatttgtttg taacagagga atatggatgt
     1321 gcttttagca tgtgtacagt acttacagat gctccaattt ctgttgaaag atatcctttg
     1381 attgattcaa aatgtatgga ttgcaatgtt tgtgtagaga actgtcctgc aaaagctata
     1441 catggaaatg aatggacttt acctggaaaa agagaatcta ttattgatgt ttcaaagtgt
     1501 ttttgcgtat tgaggtgtat gatgagttgc ccatggtcat taagatatgc aaatcaaaaa
     1561 taattttact agagaaggta ttaaattgaa tattataaaa tttttaaaat gtaagattat
     1621 ataagatttt tcaaaataaa aatttgtata gaacactcag aaatgagtgt ttttttattg
     1681 gaattaaatt atattgatac aaagcttaac tattttaatc attatataaa ctttgtatta
     1741 aaaaattaag aataacttgc taatagtaaa aatataatga gattataatt gatttataat
     1801 ataactgtaa tgatgttgta aaatgaaaat tgtcatctca aaattcccaa aaagagtaaa
     1861 agtcacttcg gttatagtca aaaaattttt tcagtgaaaa taatttagat ttaaaaggga
     1921 ggaattaatc atggaaattg aaactaaaac tccagattgg attaatgtac tagaattaaa
     1981 cagtaaaata gatattactg ggaaatatga tgtaagtaat atgatacaag atattgtctc
     2041 tgataagtca ctagaaggtt ccattatata ttttccaaaa ggtaattatt tatttgagaa
     2101 aggtattaaa attagtcaac aaataacact tcaaggagat tcttattatg gaggagataa
     2161 tcaagtatca aatttagata aaaaacaatc aatcatagga acaaccaatt ttataactcg
     2221 aggagtttct aatatgagta ttattgcatt gacaggaaca agtcaatgta tcaaaaatat
     2281 taatttttat tatgatagtc atgatataga gaaaataccg cctaaaaatg tatctgctat
     2341 tacagaatat ggagaaacac aaggactatc tcattttgaa cacctgttta tttctggatt
     2401 ttcagggata ggaattgaaa ttccatacta ttcaactggt aatgatatta ctgttagttc
     2461 atgtggtctt ggtatgaggc ttggagagaa gtcaatgctt tctagtagca agatttacga
     2521 atgtaaaaat ggaatggaga ttactactgg tgttagctta aataatgtga gaatagaaga
     2581 aataaaagaa ataggtatta ataataaagg atttggtttt aacctgatta tgaatcttat
     2641 ggtagaccaa tgtggatatt atggatttat gtttgaaaaa atgtgctata gccagataac
     2701 tgccagaatt actagatgcg gtcagtatta taaaagtgtt gattataatg cttatgagaa
     2761 tatggaagat aggaaagaag aagcttattc cattttttat ggagatattt tagaaaattg
     2821 caatattgta ttgatgaata gtaatattga taccttggaa aatgattcac ttgatgaaca
     2881 taagatatat gtaatcaagg caaatgaaac aaacaatgta atgctaacat gttatgctga
     2941 agcagataac tttatacaat gtgcaaaagg taacttgctg ttagaaaatg gcgtaaatac
     3001 ttataaattt tataatggtg agatttgttc tgtaggaggt gttcaaattt ctgatataga
     3061 tgaaaaagat ttaatcaaaa taatcgagaa cactctgtat atagatgata aaggtaagta
     3121 tttaatggtt cctagattaa aaaagaatac tgtaatatat tcagtactag attctacaga
     3181 agaaataaat aaattacatg gtggaatatg ggaacagatt gatgtaaaag tattagctgg
     3241 ggaatccatg tactattata aaaagataaa agattaaatt atcaaactag gaggtagtga
     3301 ttaatggaag agtctataat aattaaagat ataagtggct ggattaatgt acttgattat
     3361 ggaatttgta atgatgaaac aaaagatgta agttttaagt tgcaagagat tatggatgct
     3421 gtaccagaag attctattat atattttcca aaaggaaaat acttatttga aagtggtgtc
     3481 aagattaaga aacgattaac cttatgtgga gattcttata ttttagcgaa tgttcctaaa
     3541 aatactgctg gagtaacaca gtttactttt aaaggaaaga aaaatcctaa aagtgaaaat
     3601 atagcaatca taacagcaaa aagtgtaaga cactgtatta agaacatttg cttttattca
     3661 gatagttgtg atagagatat gacatctaat catgaacctc caacagatgg aaaacctaga
     3721 tttcatcata atgtaattat aaattataga aatatatctg ctgttatatg tgaagataaa
     3781 cagggagaac caggtcatta tgaaaatcta tcttttagtg gtttttctgg aattgcactt
     3841 gatatgccta ataattctac agcaaatgat attacaattt ttacatccgg acttggaatt
     3901 agtacagggg aaaattcaac aattgctaac agtaaggttt ggggctgtgt tgatggaatg
     3961 ataatttcta ctggaacttt tataaatggt atgagggtag aagaaataaa aaaagttgga
     4021 attaaaaata ttggtagagg tttaaatttt attacaaata tcacaattga ccaatgtgga
     4081 tattgtggag tttggtttga ttctatatcc aatgttcaga tatcaggaaa tatcacaagg
     4141 tgtgggcagt actattatga tgtaagctat gataattatt taatgttgga taatagagtt
     4201 aaagaagcat attctttatt ctatggtaat ttattggaat caagtaatat agttttatca
     4261 aataccaatg aagacaattg ggaagatttc aaagaggata agcataaggt gtatcttata
     4321 gaagcactta aaaccaagaa tgtactatta acatgtaata tagatgctac tgactggatt
     4381 gttaagtcag aaaaaggaaa tttaacgtac aataatatgc gacaaactgt tatattttac
     4441 gatggaaaac taagtaatat caatggaatt ggtatatctg atgaagatta tggggataaa
     4501 gtcaagataa aaaaaggaaa tatatatata aatagtaatg ataaggatat taagatgcat
     4561 aaaccagagg taggtgacat tatgtcaaca attattaaag ataaaagtat tctttctgct
     4621 cattatgggg gaacatggga gaaaattggt gaaaaaatag agtttggaat aactgtacac
     4681 tattataaat tgatagagaa ataagtaaat acgtagtatt tgaatagatg tattttattg
     4741 gagggctagg actatagttt taatgtaaat ggtttggtaa tagcatggat tgaataatat
     4801 aaaaataata taaaaaatta atttgattat gtaaatttct atgaagtagt aaaactgtga
     4861 ctttagtcat aggaggttca aactaagtaa aagggaaagg aatatataat attatggaag
     4921 gaagaactgg gattaaagat ggaagatgga ttaatgtagt agaaagaggt attaaaaatg
     4981 atggttcgta caacgtaggt cctgctttgc aggatattat tgatgaaata gaaacagatt
     5041 tttgtgtctt atattttcca aaaggtaaat acctatttac aagtggtgtt aaaattaata
     5101 aagaaattgt attgcaaggt gattctaatt catctacagg tactacacaa tttataactc
     5161 gtggagtaga aaatatgagt attattacat tgacagggaa gaaacaatgt attaaaaata
     5221 taaatttcta ttctgatagt tgtgagatac aagtaaataa agaaccaccg gcaaaaggta
     5281 atcctaaata tcattatgag atgattatca ataagaatga aaagaatgtt gagatgaata
     5341 atgtatcagc aatcctgtat gataacagta agaatatgat taaaggtcta ggtcattatg
     5401 aaaatattta tataagtggt ttttcgggta caggaattag gataccatat tactcaattg
     5461 taaatgatat tacagtttca tcttgtgggc tgggtattga tacaggaata gacacaatta
     5521 tttctaatag tagaatatct aagtgtcaaa atggaatgag gattaggact ggaactagca
     5581 taaataatgt aagaattgaa aaaatacaga aagttggtct tataagtagt caagttaagg
     5641 ggtatgaggg gtatggaagt tataaaatta ataatattac gattgaccaa tgtgggtatt
     5701 gtggcttttc atttgattca atgaaagata gttatttttc tggtattata agaaggtgtg
     5761 gtcagtatta ttataataca gattatgaca catatttaag tattaaagat agagtagaag
     5821 aagcatattc tttattttat ggaaattatt ttacagaatg taatattgat ttagttagtg
     5881 ataataagga taactgggat gatggcttag aatataaaca taaagtttat gtatttaaaa
     5941 cccttcaaat accaaaatta acattgagat gcaatataga tgcctatgat tatatcgtta
     6001 aatcggtaca tggaggaggt aatttaatgt taaaaaataa ctttaacaat tatatattta
     6061 ttgattcatc tagtccagaa gttattaatg gaattaattt taattatcaa aagaatggaa
     6121 acctgttaag gttaaataat ggtaaattaa gttataaaaa tggaaaggaa gaaacagcta
     6181 tattcagacc taattatggt cttattgtag catcaacaga ttcagaatgt gaacaaatag
     6241 aggctaccta tggagaaaaa tgggagaagc ttggttctga agtaattgga aaagaaacgg
     6301 tatattatta taaacatcat gaaccagaaa aaacaaacca aatacaataa aatttgtatt
     6361 atataatgat ataaagtttt aatatatagt gtttaccttt aatgtaatat tgatatattt
     6421 gtatagtatt aaatttgtta aaaagtaaaa ttgtagggtg cttgaaaata gcaccttttt
     6481 tagtttcatt ataaaattag ttttgaaagt ttaggaataa tgtatattta tttttaaaga
     6541 attataactt tattaaggat attttaaatg ctagaaatga atcagtaaat catactggaa
     6601 ttatcttaaa tgttttgatt aaattaagta tttagagcta taattatatt aaaaatatat
     6661 tagaacataa ttatattact atggaggtta aatatgatag taagtgtgag tagaagaact
     6721 gatatacctg ctttctatag taagtggttt tttaacagac ttgaagaagg ttttgtttat
     6781 gtaattaatc caatgaatcc aaaacaggtt agtaagatag agttaaatcc acatactgtt
     6841 gattgttttg tattttggac aaaggatgta actccaatgt tatatgattt aaataaatta
     6901 agagactata aatattattt tcactatact ataacatcat atggaaaaga agtagagaca
     6961 tgtattttag ataaaagaaa ggtaatagat tcatttaagg aattgtctag aaaaataggc
     7021 aaagaaagag taattttaag atacgacccg attttcttaa gtgaaaagta cacagtagat
     7081 tatcatatca aagcatttgc gagtttatgt aatcagcttg atggatttac agaaaaatgt
     7141 ataataagtt ttatcgattt gtataagaaa acaaaattta atactaaagc attacatata
     7201 aagccaatag aaattacaga aatagaaaca ttatcaaaag agatttctaa gattgctcat
     7261 aagcatggga taatacttga aatgtgttct gaagaatata atttaagtaa atttgggatt
     7321 ggaaaaagta agtgtattga tgatagatta atatcaaaaa ttataggatg tgaagtagag
     7381 gtaaaaaaag atagtacaca aagagatatt tgtggatgtg ttaaaagtgt cgatatagga
     7441 caatacaata cttgcagaca ttattgtctg tattgttatg ctaactttaa ttatgcaaaa
     7501 gtagaagaaa actgtaggct ttataaggaa gataataaat tactcataga tactttaaga
     7561 gatgatgcaa aaataagcat tagagatatg aagactatta aagtagataa taataaacaa
     7621 attagtatat ttgattaaaa taaatatact aattacctta atatattaag aatatagata
     7681 cttttgataa taaaattaaa cttaatagat attttctata taagaaagat ggtcataata
     7741 tgtcaatttg accatctttt tgatataatt aaaacacaaa taatttagta acaaaggagg
     7801 cagaacttga atttattaaa aaaatatcct atacctattg caagtttgat tttatcaatt
     7861 tttactttag gtaacttatt acaatcttat agtgaatcat tacgtaatgt aattggattt
     7921 ataggattta ttttgtatgc aatttatata gtcaaaataa tattacttag aaagggtgtt
     7981 aaagagcaat tagagaaccc tttaatatca agtacatttc caactataac aatggcaaca
     8041 atgatatttg ctacatatat aaaaccatta tctttagaat taagtattat gatttggagc
     8101 ataggaattg tgggtcatct tgtgttaata gtcttattta gtaagaagtt cttagttaaa
     8161 ttctcaataa aaacggtatt tccatcttgg tatatagtat atgttgggat agttgcagct
     8221 agtgttacgg caccagcatt taatcaactt ttgattggca aaattgcttt ttggatagga
     8281 tttgtatctt atatagttct tataccagta attttaaaga gagtatggat aatcaaggaa
     8341 atgcctgaac catctttacc aagtatagct atattagctg caccagggtc actattattg
     8401 tctggatata ttaattcttt ttcagataaa aataatgtag tattatactt gttattcata
     8461 ttatcaattg tattttatat tatagttttg ggatatttac caaaattatt aagattgcct
     8521 ttttatccga gttttgctgc atttacattc cctatggcaa taagtgcaat gggtatgaaa
     8581 ctaatgaatg gttatttaac taaatcaggt aattttataa gttggatttc atatgcttct
     8641 aagttagaag agataattgc tacagtgatt attttatatg ttttagtttt gtttattata
     8701 tatcttagta aatctgaaac tacatctaaa gctaaataag aattattaaa tatgtgtatt
     8761 taataggaat attgatataa atatatgaag ataagataaa ccatagaaac ttaatttagc
     8821 ttaattttat tggaggaaaa caaatgaaaa caaaagaaga atatttaaaa gaagttagaa
     8881 acatatcaga agaatacttt agaaaaggag agtttttttg ctctgaagca gttctccaga
     8941 caataaatga tgctttaggc caacctttaa gtccagagat aactaagtta gcatcaggat
     9001 ttccgatagg acttggaaag gcacaatgtc tttgtggagc aatatctggg ggagagatgg
     9061 ctttaggtat agtatatgga agagttcatg gtgaagctat gaatccaaag atgtttgagc
     9121 atgctaaagg tttgcatgat tatattaaaa aagagtatgg tgcaacatgt tgtagagcta
     9181 ttacaaagaa atgggatggt gataacttta tgtctcctga aagaaaagag cactgtatta
     9241 aaataacagg tcaagttaca gagtgggttg cagataagct tatagaagat gaaaaactta
     9301 atatcaaata gtgtaatatt agttggtgaa tttataaagt taaatttact tatagtataa
     9361 gatacttgat attctcgtat tgatagaaaa atttatagca tatgtattta atgaaatata
     9421 cttatattta gagtgtggtt taatttcata aatacatatg ctattatttt aagtatataa
     9481 gcaaatttat ttaaataaat agaaacagtt aatttatatg tacaaaacta tatatgatta
     9541 gtgtaaataa atacctattt tataatactc aacataaaga caccagacat gattagtatt
     9601 ataccaataa aaatagcaag ccattctttt ttgttcttct tttctttaaa aaatataatc
     9661 cctcctaaag tggctactat cacacaactc tgagagatag aaaaacttat tgtaactccc
     9721 aatgtattag tcgttatgaa catacctaaa tttgctattg accacaatat accagtaatt
     9781 aaattgaata aaacattttc cctatataca atctctttag aaaaatactt gacaataata
     9841 gatgaagtta tcaacatacc aacagattga ggtaatataa ttctgtatcc ttcaattaaa
     9901 aatatttgat tagttacaac atataatgat aaaaatagag atgataataa tacaactcca
     9961 acacatttta acatattttt atctgtagat tttttagttt gataatttgt gagaagtata
    10021 ccaagtatta tacataatat tgataaagaa ccaatcagta acattttaga actggtccat
    10081 tcgttaaaca agattacagc taataatgtc gtaaaagtta attgtgaacc atttgaaatt
    10141 ggcattactt tagatacact agcaatttga attgctttaa actgcattaa ttgtcccaaa
    10201 ctccagaata tgccagaaat catacttata caaaatgaaa aaaatgtaat ttcaggatgt
    10261 ataaccagag ttattatagt ggaaaatata aacgcactta tggatgtacc taaaagctgt
    10321 tcatgaggag aacttttctt tagatttgca actatgggca tcaatcccca tcccattaca
    10381 ggaagtaaag caataattat tgtcagcatt aaactatttc ctttcatatt aaatttagac
    10441 agcttttaat ttatcatcta aaattaaagt gttcaataca taaaaattac cgtatttaat
    10501 caggaaatat tagtagtttt ttagaaaaac attttccatt aaaataaaga caaagggaag
    10561 gagtaaaatt gaaaatgtta actgatatga ttcaaaatcg tgaaattaaa ataataaagt
    10621 ttttattaaa agaaggtcat acaactgtaa aagcaatagc agatgattta gatgtttcag
    10681 aaaaaacagt ttcaaaatct cttaaagaag tagaagcagt gcttaaaaag ataggtcttt
    10741 ctttaataag aaaacctaag ataggtgttt atattgatgg agatttaaaa aaaatacttc
    10801 cttacctaga taataaagga agtcaaattc ctacaaccag agaggagaga gttatctata
    10861 tatattcagt acttttaaag agtaatgatt atattactat acaacaactt tctgatgaac
    10921 tatatataag tcgtggaact attgaaaggg atttagcaga agttgaaaaa ctgctaaaaa
    10981 acgaagggat ttccatatat aagaaaccta gtaagggaat aaagttaaat ataagtgaaa
    11041 gagataaaag acaacttact gctaaattta tacataaatt ttggagtaga aactggtata
    11101 ttaagcaaga gcaagaaagc ttttatcaag catttgaaaa gatacaagca gatgtaaatg
    11161 gtattttttc ggaagatgat ttatcaataa ttattgatat tcttaggaat ttcagcagag
    11221 aaagaaaatt tgagttctct gattatgaat ttcagtcatt agttattcat ttggcaatag
    11281 ctgtagagag aattagaaaa ggcgaatata tagaagaaag tttgtatact ggtaatatag
    11341 aaaataatca aatgtcaaat accaattatt tagcaaaaca attagaagaa aaattttcaa
    11401 tattattacc aaaatctgaa attggatata taaatttaca tttagtagcg tctgaccaac
    11461 atagagatac taatgaacta aaaaatactt caactaatcc ttgtgagaca agtacaaata
    11521 atatatcaaa tcttcgggaa attataaaaa atacattgta tgagacagct tatgacaagg
    11581 atttaattga tggattaact tcacatgtac attcatcaat aaatagactg aaatatggac
    11641 ttagtattaa aaatccatat gtaaatacaa tcaaacaaaa cttttcttta tcatttgagc
    11701 aagctttaga tttaaagaaa gtaattgaat ttatatatga tattaggata aatgatgatg
    11761 aatgtgctta tatagcatta cattttgaag cttttagaga aagaatgaaa gaacttccac
    11821 aaaatattag agtaattcta gtatgcagta caggattagg ttcatctagg ttattgtctg
    11881 caagaataaa aaaatacttt ccaatgataa agattgaaaa agtactttcc gtacaagaat
    11941 tagtatccag taaaattgat gcagatttag taataagtac tatttattta gagttagaaa
    12001 caattccaac catagtagtt agtcctattt taaatgaaag tgatataaga accttggaaa
    12061 gaaatataca aaatgtttca agttgtactt ctaagaagta taaatctttc agaaatttaa
    12121 tctttgaaga aaatatattg ttgaatattg aagcttcaaa ttatgaagat gcaattaatc
    12181 atataacaga taacctaata aaaaaaggtt ttgcaaaaga aggtatagct aaaagtgcaa
    12241 ttgagagaga aaatttatct tacacttctt tccaatcaat agctacacct catgctaatc
    12301 ctagttatat taaaaaatct aatatatctg ttgcaacgtt aaaaaatcct attacatggg
    12361 gaaatgaaaa ggtggaagtt attttcttta tttcattaga aaatgacaaa aacctagagc
    12421 ttgatgaaat atatgaagtt ttttatgact ttattgacaa taaaaaaaat ataaatgaca
    12481 tattaagtgc aacttccaca aaggaagtgt acgatttgct agtggaggag agtatatagt
    12541 gaaaatatca gatgtattga aaaaagagca aattgttttt aatcttgata caaatacaaa
    12601 ggaagaaaca ataaaaaaat taagtagtat atttgttgaa aatgggattg tggacaatga
    12661 agaagaatat gttaagtctg ttttggaaag agaagaacat tcaactacag gtattggtaa
    12721 tggtattgct attcctcatg gaaaaagtaa tgctgtcaaa aaatcttcta taatatttgc
    12781 aaagactaag cataagattg aatgggatgc tttagatgaa aaaccagtag atagtatttt
    12841 cttattggct ataagcaata ttgataagga tagcaatcat cttgtactat tatctaaatt
    12901 agcaactaaa cttatggatg atgacaatgt agatgcacta aaattagcca attcagaaca
    12961 agaaataatc aatatattta gtgaagaagg agagatgtag aaatgaaaag aaaaattata
    13021 gcagttacag catgtgcaac aggagttgca catacatata tggcagctca agcgttaaag
    13081 aaagcttcaa aaaagatggg tcaccttata aaagttgaaa cacaaggagc tacaggaatc
    13141 gataatgagt taactacaaa ggatgcacaa attgcagaag tagtaatatt tgcagtagat
    13201 actaaagtca gaaatgctga acgttttgag ggaaaagaaa tactagaagt accagttaat
    13261 gcacctataa aggatgcaga gaaagtaata aatgatgcac taaagttaat tgacactaaa
    13321 taaatggagg gaaatataat gttgaaaaag atagtctctg agttaaaaaa gcatgtctta
    13381 actggaatct catatatgat tccattagta attgcaggtg ctatgataat ggcgatatct
    13441 cgtgtaggtg gttctttcta taatatacca gatatatggg atgctaaata tgcagaaagt
    13501 gctagtagta ttgttagatt attacatgat ttagatgggt ttggaggtac tgcattagga
    13561 ttaatgtttc ctgtaattgc tgcatttata ggattttcta tagctgataa attagctatt
    13621 gtaccaggtc ttgttggagg tatgattgct aaagatatag gtgcaggatt tttaggagct
    13681 ttggcagttg gtcttatagc aggatataca tgtttgttta ttaaaaatca tgttaaatta
    13741 ccaaaatcag cagcttcaat agtaccaata tttatagtac cagtatttgg gacattaatc
    13801 actgttgtat taatcaatta tgtgataggt ataccttttg ccacattaaa tactggatta
    13861 gaaaattggt taaatggatt atctggtaca aatcagatat taatggcagc tattataggg
    13921 gctatgatgg cagttgactt aggtggacca gttaataaag cagctttaac aacatcactt
    13981 gcattattaa caagtggaat ttatgcgcct aacactgctg caatggtagg aatagttata
    14041 ccaccactag gattgggact tgctactata ctagcaaaac aaaaatataa taaacagtta
    14101 cgtgaagctg gaaaatcatc tcttataatg ggtttaattg gagtaagtga aggagcaatt
    14161 ccttttgcag tagaatctcc attaaaagta ataccttcat gtgtaattgg aacagcaatt
    14221 gcttcagcta tggcagttgg tttaggttca gtaaatgcaa ctccaattag tggattttat
    14281 ggatggttta cagtagagaa ttggccaatg tatgtgttat caatagcagt tggaacagta
    14341 attgtagctg gattagttat agtattgcgt ggaaatccag tgattgagga tgatgaattt
    14401 gaagatgatg attttgatga agcagagtgg gaatcttaaa atttaataac tatttaagac
    14461 gtgttataag ctatattcaa tatgaatctt atactaaata aagtgttaaa cattcagttt
    14521 agacttaaat ataaaagata aagaacagag gagaataaat gataaaagca catattgtta
    14581 atcatacaca ctgggataga gaatggtatt ttacttctgg agatgcatta gtacttagtg
    14641 aacaactatt tacagatgta attgatgaat tagaacgtaa tccagatgta agttttgtac
    14701 ttgatggaca actttctatt ttggatgatt atgtacaatt acacaaagaa aaaatagaaa
    14761 tcattaaaaa gttaataaaa gaagatagac tacatattgg accatggttt actcaaactg
    14821 atgcattctt tgctagagga gagtctattt taagaaactt aatgattgga atttttgaaa
    14881 gtaaaaagta tggtaagtat atgaaaattg ggtatttgcc agatacattt ggatttaatg
    14941 cacagatgcc tatactatta aagcatgtag gtcttgataa tatattcttt tggagaggta
    15001 tacatcttgg taaacaggtt caatcaccat attttaagtg gaaaagctta aatggagaaa
    15061 gttatatata tgcagtaaat atgccacatg gatatggaac tggtatgctt ttagagcctt
    15121 cattaaagta tgttaatgga cgcttagacc cagctattga ttttattaaa tcatatactg
    15181 atgtagatga ggtgttaata ccttctggga atgaccagtt aaatataata ggtgatttca
    15241 aaaataaaat taaagcgatt aatgatatcg gaaagtattc atatataacg agttcatatc
    15301 aagagttttt aaaatatata aagtctatag aatcactaga aagctataga ggtgaattta
    15361 gagaacctgt attagcaaga atacataaat caattggttc tagtagaatg gatataaaat
    15421 tagcatgtga taaactagaa aacaagctga ttaaaagaat agagccatta ttagtaattg
    15481 ctaaaaagag taaaattgaa ataagcaatc agttacttat aaatacatgg aaaaaacttt
    15541 tggaaggaca agcacacgat agtttagctg gatgtgtaac tgatactgta acagatgata
    15601 ttcttcaccg tataagagaa gcaaatgaaa tatgtgacag tattgaaaat actattgtaa
    15661 aaaagatttc agaagggctt aaattatcta aaaatgatat tttagtattc aatacagaag
    15721 ctaagcgttt taatggatat aaagagatac aagttgtaag tgatagtaaa aatatttatt
    15781 ttaaagataa tttagatgct actataattg aagaaactta tgtaaaaccg agagaaaatg
    15841 tattagaaga aacacctgct ggtaatatat ttattgaaga accaggatat tatatattaa
    15901 aagtaagaat aaatatacag cttcctgcac ttggttataa agttgttagt tttgaattaa
    15961 gtgataaaga aatggtgtca cttgataaat ctaatgacac ttttatatct aatgataatt
    16021 ataagataat atatgaagat ggaagcttaa atttacaact taaagatgga acttacatat
    16081 caaacttttt aacattaaaa gatagtggta atgcaggtga tacctatgac ttttctccac
    16141 taaaaaatga tgaggatata aaactttcgt ttgataaagt gtatacagaa aaagctttag
    16201 gatatgaaaa aatggtggtt aaaggtacta ctttactacc acttacatta gaagatagaa
    16261 aaaacaaata tcttaatggg aaattaaatg taaaagtaag tattgtctta tctaaagaaa
    16321 acccattgat ggatgtaaaa ctttgtgttg ataacacgat atataatcat agactacgtg
    16381 ttcatataaa aactgatata aaagataata aaaacatagc atcattgcca tttggatata
    16441 taacaagaga aaatggagtt ttagacaact gggaagatat ttacagtgaa atgcctatag
    16501 atttagaacc tttggaaagt aatattacgc ttacaaacca aaatagaagt tgtactgtat
    16561 ttactagagg aataaaagag tatcaacata ttgaagatga aattgcattg accttacttg
    16621 ctactacaga tgaactagga aagcctgatt tattatatag accaggacgt gcttctggag
    16681 atacaactaa aaaaggtcat ataagaatta aaacagataa agctcaaatg cttaaggaat
    16741 tagaattttc atttgcaata tatatggatt ctaaatcatt tgatgaattg gaaattgcga
    16801 atttaaccca caattatcta agagaatgtg taaattatca attacaggat tacaatttct
    16861 ttttatatcg tatagataat aagattcaaa aatcaattgt caataaaact gtaagtagag
    16921 aattagaaat aatatcttta ccagaaaact atcttatatc agcatgttat ccttcttatt
    16981 acagtaaaga taaatttata ataagagtgg aaaatccgag tagtaataga atgatattgg
    17041 atgagcaaat atttaaaaat agaaatggga aaattgtaaa tgcaatagaa gatgtagaag
    17101 aagaacaggt atatgaaata gcaccttttg acgttatttc aatactattg gatttataag
    17161 tgtaaaagta agtttattgt ttaaagtctg acagatatta atctgccaga ctttttaatt
    17221 tgtataaatt tgggtaaata ttaatgaatg gttttagtat ttaagtatga atttagtatt
    17281 taatatagaa tttattgaag tagaataatt agtttaacag gataagcagt ttaaataaag
    17341 aattat
//
LOCUS       DAMJVI010000081        17318 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.083, whole genome shotgun sequence.
ACCESSION   DAMJVI010000081 DAMJVI010000000
VERSION     DAMJVI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.083"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            105..467
                     /locus_tag="PC545_002695"
     CDS             105..467
                     /locus_tag="PC545_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDF2457458.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPSKEELKS
                     IYKTSGYDIKKFFNTSGMKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLLDGKN
                     VLLGFKEDVWKSTLLKED"
     gene            903..1604
                     /locus_tag="PC545_002696"
     CDS             903..1604
                     /locus_tag="PC545_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HDF2457459.1"
                     /translation="MSIEDLYDFECVPLNNEIDIDVLFEKMIEDGKEKEYTPIIVIDE
                     KVGLLRKNIDMITKEYGSLENYREYCLTKYNEIDVNQFFDFRKSEISELLKELIDLED
                     DFYDYKHFRPIMNIDIFHEYNKVYIAKIPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKNLTKDGKKFDDLCIELVFYCEDIIIQGYETLKAL
                     KDVLKRSTIVLFWWD"
     gene            1874..2692
                     /gene="larE"
                     /locus_tag="PC545_002697"
     CDS             1874..2692
                     /gene="larE"
                     /locus_tag="PC545_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HDF2457460.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVIAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGVDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSSLKKEE
                     IRSLSKTLGLNTFNKPSFACLASRIPYGVEITDENLRIVEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            2747..3895
                     /locus_tag="PC545_002698"
     CDS             2747..3895
                     /locus_tag="PC545_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDF2457461.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            4031..5419
                     /locus_tag="PC545_002699"
     CDS             4031..5419
                     /locus_tag="PC545_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2457462.1"
                     /translation="MKVLKLITVVALMMLFVTGCNIKTESPEQLAKIPDYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGK
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGSTGNNKNTYSSKSSATIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTGTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            5448..6146
                     /locus_tag="PC545_002700"
     CDS             5448..6146
                     /locus_tag="PC545_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457463.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     EKDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALLRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            6149..7582
                     /locus_tag="PC545_002701"
     CDS             6149..7582
                     /locus_tag="PC545_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457464.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVASQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            7604..8341
                     /gene="truA"
                     /locus_tag="PC545_002702"
     CDS             7604..8341
                     /gene="truA"
                     /locus_tag="PC545_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDF2457465.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKTVEEVDERFHS
                     RYNVKSKTYMYKIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNVIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESKDRCQSSDTAPAKGLCLWKVTY"
     misc_binding    8452..8659
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8912..10111
                     /locus_tag="PC545_002703"
     CDS             8912..10111
                     /locus_tag="PC545_002703"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDF2457466.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEVYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYEAHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     gene            10358..11404
                     /locus_tag="PC545_002704"
     CDS             10358..11404
                     /locus_tag="PC545_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4872 domain-containing protein"
                     /protein_id="HDF2457467.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGEYLPNDFFFLLSGF
                     GSFCYMKTNKADLKRMVALGDGRTKQMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DDGYPVILGALDMYYLPYYKKLYHKEHIPFHYILMVGYDDEGIYLYDCGRTELLHLSY
                     DKLRESMNCNSPGLSKENTICTIRMNTSKNKYNIAREAIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELPQWNTELDKDDYDKILTNMVMFFGTVPTIPNAIKGIKEPDTVQFKGGFDK
                     MSRMLNSIGEEYKNNSFIQVADIFDKTAIIIEKISNIIIDYLTQTCDDTEQLPLLFSE
                     VLEHMKTGYLILDV"
     gene            11512..12675
                     /locus_tag="PC545_002705"
     CDS             11512..12675
                     /locus_tag="PC545_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2457468.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDES
                     IKTLQQILFFKEIDIPLREVKEIMSSQYFDKVEALKNQKKLLILKRKRLDELIELINQ
                     TLRGEGNIDFKEFDMSEYFNVLEEFKREHRNKVIKIYGSVEKYNEYIERVKSNEEKIA
                     KMAIKQYGTIEKFAKAIKTNFSSDILNLGEKFDRYKNDCLKEKHPKLKELYRKLVEDL
                     SRNPSSTDLQEIAKAITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSREVQSIVEEIDNEMKRSNE
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGDGASKFIGEAFKIYFDNNNC"
     gene            complement(12764..13144)
                     /locus_tag="PC545_002706"
     CDS             complement(12764..13144)
                     /locus_tag="PC545_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HDF2457469.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKDNISVLEKSIE
                     NEDWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EVESIKTYLGNIIESVKFDVNYIF"
     gene            complement(13147..13806)
                     /locus_tag="PC545_002707"
     CDS             complement(13147..13806)
                     /locus_tag="PC545_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDF2457470.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERISIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            complement(13912..14886)
                     /locus_tag="PC545_002708"
     CDS             complement(13912..14886)
                     /locus_tag="PC545_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2457471.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGAELISIGGVLVDNEFNTI
                     DNYYSLVKPKYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDITGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAVDLMNVCKCFFFNSPKNIDILEELYNKLPALKTPLYYIPYFED
                     ENFELKFDRTPEDIIIYLKQILTILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKLNEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            complement(14926..15273)
                     /locus_tag="PC545_002709"
     CDS             complement(14926..15273)
                     /locus_tag="PC545_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457472.1"
                     /translation="MLNLKEIREEIIKKYIGDSLISELTEELKNILITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILIDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRVIISIDSYDA"
     gene            complement(15491..16447)
                     /locus_tag="PC545_002710"
     CDS             complement(15491..16447)
                     /locus_tag="PC545_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDF2457473.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRVKEGNPVC
                     ESGIFEILDVTDKNRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EIAKELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            16890..17258
                     /locus_tag="PC545_002711"
     CDS             16890..17258
                     /locus_tag="PC545_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HDF2457474.1"
                     /translation="MTRPSIYHSQGYTCAEALIKSYNEEHNTDIPISIGSGMGVGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELLNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
BASE COUNT         6837 a         1807 c         2803 g         5871 t
ORIGIN      
        1 gataattttt tatgttgaaa gcttataatg attatgatat aaattggtta tagtatacaa
       61 tttatgggta taacaataaa aaattagaaa ggaattttta taaaatgatt aaattatatg
      121 gatatacaaa atgctctact gttaaaaaag ctaaaaattg gcttaaggaa aataatttag
      181 aatttgaaga tatagatatg gttcaaaacc ctcctagtaa agaggagtta aaatcaattt
      241 ataagactag tggatatgat ataaagaaat tttttaatac aagtggtatg aaatatagag
      301 aattgggtct taaagatatt gtaaaaactg aatctgatga taaactttta gaaatattgg
      361 taagtgatgg tatgcttatt aaaagaccat tacttttgga tggtaaaaat gtattattgg
      421 gttttaaaga agatgtatgg aaaagtactt tgctaaaaga agattaggaa gagataggtg
      481 tgttgattaa aattattaat taatacacct ataaatttat gtattaaatc taatctttat
      541 aaatattaat tagcaaattc aaatcaaaaa aattcatatc aacaaacata ttacttctta
      601 ttacaaatta tatcatatgt tttatattaa aatattataa tattacaacg aattgttaca
      661 aaattactta ttattaacca tgaacatttg ttttgtgcta tcatatttat ataagggaat
      721 tgtcataaaa aatatcttgt tcatacagtt ttatatagaa aaaagaaatg aatcttttct
      781 ttatataagt atattttgta aattgcctct atatttatag tttaaataat agtggtatta
      841 aaaatctgac aacaaaaata aaactttcaa aaaattaaat ctaaaaagaa taggagataa
      901 atatgagtat tgaagattta tatgattttg aatgtgttcc tttgaacaat gagattgata
      961 ttgatgtttt atttgaaaag atgatagaag atggaaaaga aaaagaatat acaccaatta
     1021 tagtcattga tgaaaaagta gggctattaa gaaaaaatat agatatgatt actaaagagt
     1081 atggttctct tgaaaactat agagagtatt gtttaacaaa atataatgaa attgatgtaa
     1141 atcaattttt tgattttagg aagagtgaga tttcagaatt attaaaggag ctaattgatt
     1201 tagaagatga tttttatgat tataaacatt ttagacccat tatgaatata gatatatttc
     1261 atgaatataa caaagtttac atagctaaaa ttccaacaat taaaccatat gaagtatttg
     1321 catacatacc tattggcgga tttaatgatt gtcctagaga tgaagaacat atagctattg
     1381 caaaatattg gtatgaaaag catggaatct ttccaatcgc aattggatgt gatacagttc
     1441 aatattctgt aaaaaattta actaaagatg gtaaaaaatt tgatgactta tgtattgaat
     1501 tagtatttta ttgtgaggat attattatac aagggtatga aaccttaaag gctcttaagg
     1561 atgtattaaa acgctctaca atagtgcttt tttggtggga ttgatattct atcatttaga
     1621 tagtattaaa ttgttagtca ccaagaatcc acttgcttta gttataggga gtgtgtcaaa
     1681 ctaggttttt atacacagtg tagcatagta atatgtttaa attacaaagg ttaaaaaaat
     1741 actttaatat ttttaatatt ataattaata gatttaaggt aattataatt tataaaataa
     1801 atactttata tgttataatc attaagaatg taaaaaaata atatatagtt ttatagttca
     1861 tatttttcca gatatggagg taaattttgt gaaggaaaaa gaaaaattag ataaattaaa
     1921 aaaaatgtta cttgaattag gcagtgttgt agttgcttat tctggtggag tagatagtaa
     1981 ttttttgctt aaggtcgcaa aagatacatt aggtgaaaat gttatagctg tgactattca
     2041 tgcaatgatg cattcaagta gggaaattga agaggctaaa caatatactc aaaattttgg
     2101 tgttaaacat atcatattaa atatagaaaa ctttgattta aaagaattta aagaaaatgg
     2161 tgttgataga tgttatcatt gtaaaaaata tatattctct aaaataaaag aggtagcaaa
     2221 agaacataat ataaagtaca tagtagatgg aacaaatata gatgatttag gagactatag
     2281 acctgggctt aaggcattga gtgaattagg agtaattagt ccattaaaag atagtagttt
     2341 aaaaaaagaa gaaattaggt cgctttcaaa aacattagga ttaaatactt ttaataaacc
     2401 atcatttgca tgtcttgcga gtaggatacc ttatggagtt gaaataacag atgaaaattt
     2461 aagaatagta gaaaagagtg aagaatatct atccaattta ggtttttctc aatttagagt
     2521 gagaatgcat ggagatatag ctagaataga agttggacag gaagagctag gtaaattttt
     2581 tgaaaataac aattttaata aagttgatac taaattaaaa atatttggct ttaaatatgt
     2641 tacactagat atgtcaggat ataaaatggg aagcatgaat ttaaatgtgt agattatttt
     2701 aaaaataaat aagttttata gttaatataa ggaaacagga ggttttatga aaaattatac
     2761 gttaatatcg ccatgttttt ttggaatgga aaaaatgctg gctagagaga ttactaattt
     2821 aggatatgaa ataataaaaa cagaagatgg aagaataaca tataaaactg atgagtttgg
     2881 aatagcaaag tcaaacatgt ggttgagatg tgctgaaaga gtacatctta aaattgctga
     2941 atttgaggca aaaagctttg atgaattatt tgaaaataca aaaagaataa actggtctag
     3001 atatatacct tatggagctc aatttcctat atcaaaagct tcctctataa aatcaaaatt
     3061 atacagtaca ccagatgtac aagccatagt aaaaaaggct atagtagaaa gcttaaagaa
     3121 aagttatttg gaggatggtt tgcttaaaga agataaggag aaatacccta tttttgtatt
     3181 tatacacaaa gataaagtaa ccatttctat agatacaaca ggagatgctt tgcataagag
     3241 aggatataga gaaaaggcaa ataaagctcc aataagagag actttagctg ctgggcttat
     3301 ttatttaact ccctggaaag caggtagagt tttagtagac cctatgtgtg gttctggtac
     3361 tatattgatt gaagctgcta tgattggtat aaatatggca cctggcttaa atagagagtt
     3421 tatatcagaa aagtggagaa ctcttgataa gaaaatatgg tgggatgtaa gaaaagatgc
     3481 ttttaataag atagataatg aatctaaatt taagatttat ggatatgata tagatgaaga
     3541 atctatagat atagctagag aaaatgctga aatagcaggt gtagatgaat atatagagtt
     3601 taatgtagga gatgcaactc aatttaaaag tgaagatgag tttggattta taatcactaa
     3661 tcctccttac ggagaaagat tagaagataa agacagtgtt aaacagttgt ataaggagct
     3721 tggttatgca tttagaaaat taaaaaattg gtcgtattat ttgataactt cttatgaaga
     3781 ctttgaatat gagtttgggc aaaaagcaga taaaaaaaga aaattgtata atggtatgtt
     3841 aaaaactaac tttttccaat atccgggtcc taagcctcct agaaataata aataagtttt
     3901 acatgattta tttgtaaaat ataaaatttt attaaaataa gtaaaacatt atgatgccct
     3961 gaaacgtcta attattgtga ggagattttt gtgaacaaat ttaaagattt ataggattag
     4021 gaggagttaa atgaaagtgt taaagcttat tactgtagta gccctgatga tgttatttgt
     4081 tactggatgt aatataaaaa ctgaatctcc agaacaatta gcgaagatac ctgactatga
     4141 tgatacaaaa aaagtattgt atgatggtat tgaccagtta ctaaaaccag attcatctat
     4201 gatattgcct tctaatacaa aagaagtagg aaaaataaat aaggtagatt taaacagcga
     4261 tggaattgat gaattagtag tatttgaaca aaaagaagat ttaagtaata atgtgagtca
     4321 ggtagggttt gtaaccttga gttacaatgg agaaaaatac gtacttggag accatttttt
     4381 agaaaatggc gaatcaatag aatatgctaa tttttatgat ttggactcag atggatataa
     4441 ggaagttatc ttattggtta aatccaaaga caaaacgaat atgcatatat ataaagtagt
     4501 agataatgaa ataacaaaaa tatatgactt ggatgcatca tggcttcaaa ataaagaaga
     4561 ttttaatgac atgaaagtaa aaattggata tatagatgga gatgacaagt tagacatatt
     4621 gatgcttcat ttgaataata agactaatga aatgtttgca agtgttgcaa attttgatgg
     4681 gaaaatgaaa atcaaagatt ctgttaagtt tgaaaatgta aaaaacttaa gtgaattata
     4741 tataacatta ggtaatgttg cttcatctgt tggaaattct tctgcagcta taaaaggtat
     4801 tgtacttgat attcctatat tgaaagataa taactatatt acgcagatac tttatatgaa
     4861 agatgggaaa attaacaaag cttttagtga ttatgataaa actattacta aatcttatta
     4921 tatacctgta gatgatattg acaaagataa gattatagaa atacctatag ttgcaggcag
     4981 tacaggtaat aataaaaata cttatagttc taaatcatct gcaactataa gttggtatag
     5041 atggaatgga aaagaaggtg ctgactcaag tttaatattt acaagtcaga tatattataa
     5101 ttataaatat aattttaaat tatttattcc aaataattta tttgataaaa tacttgttga
     5161 acaggaattt gttggagaaa aagcagtatt taaattttac tattttgact attcagaccg
     5221 taagaattta tttaatatag tagtagagag taaaaataaa cttgaagata gaaaaaatac
     5281 aggtactcaa aattctattg tacttcaaga gagcgatgaa tatactttct tactagttgt
     5341 aaacaatgca aatgaaatgg ataagctaga tatgactata gacgcattaa aagaatattt
     5401 ctcattaata tatggttaat attatactca attggaggtt ttattttatg aaggaaaaga
     5461 tacttatact agaagatgaa attggtatta gaagttttgt tagtataaac ttaaaaaggg
     5521 aaggctatga aatagttgag gcaggtactg gaagagaagc tatagaaaaa atgacaacag
     5581 aaaaggatat tacaatagct cttttagatg taatgcttcc agatataagt ggtatagaag
     5641 tatgtaaatt tataagggag aattttgacc aagtaggtat aataatgctt actgctaaag
     5701 ctcaagaaga tgataaaata gaaggattta tctcaggagc agatgattac atcataaaac
     5761 catttagtat aaaagagctt ttagtcagag tttctgctct tcttagaaga gtagcaaagg
     5821 atgattcaag tgttaagtcg agtgagatag tttctccacc atttatactt gatatagaca
     5881 aaagaaaatt atttaaaaat ggaaaggaaa ttgagcttac accaactgaa ttttcaatag
     5941 tgaaatatct aatatcaaat gctaagcagt cgttaagtag agaccagata ttagacgaag
     6001 tatggggaac taactattta tatgatttta aaatagtaga tgtaaatata agaaggataa
     6061 gaaataaaat agaagatgac ccatcaaaac caaaatatat acaaactatt tggggttatg
     6121 gatactgttt tagaaaggaa gaataatagt gttacatttt gaaggtctga gaaaaaaagt
     6181 aataaaaaac tattttataa taattattat aatggttact ctgtttgaag gattgttcat
     6241 gttttacata caaaactact attatgattc tgtcaaacag ctacttgaat ctgaaataaa
     6301 atatgctgat gaatataatg ctattacaat ggaaactaca agttttgaga aaaaagtaaa
     6361 aaatatattt gataaacaac ccttaactaa aaattcagaa tttggaattt caataataga
     6421 taaagataaa aatattatat tagaccaata tggttttaaa agtaaagaaa aagcaaacta
     6481 tgaagatgtt aacaatgcat taaaagatat aaaaactaaa aatttaacac cttatactta
     6541 tagaatacca gatacagggg agcatgttat gagtatctcc ttaccactta aggtaaataa
     6601 tataatagaa ggtgttgtta gatatacagt atcattagat gctattgata atgcaatatt
     6661 aaagcaagct acatggctta tattagcagg aatatttata cttattatag ctatactaat
     6721 aagtcttaaa tttgctgaga ctcttataaa accattacga gagctaaaga aatttgcaaa
     6781 tgaattggct gtaggaaatt ataatataaa attagaaaag atgaagattg tagatgatga
     6841 aattggtgat ttagcacaga catttgaaca catggcacat gagattgaca aaagtgaaaa
     6901 attaaaagag gaatttatat catctgtatc tcatgaacta aggactcctc ttacatctat
     6961 aaaggggtgg agtgaaacct taggttatga aagtataaca agagaagaac tagacttagg
     7021 tttaggcatt atacaagatg aaacagaaag acttataaaa ctagttgaag aattattaga
     7081 cttttcaagg ctttcttcag acaggataaa acttcatgtt gatatagttg atgttgaagg
     7141 tttaattgta ggtgttgtaa atcaacttaa agttaaagca gctgaaaaag atatatcact
     7201 tttatttgaa tttgaaaatg aatttataga gaacattcaa ggagataaaa atagattaag
     7261 acaggttctc ataaatttaa tacaaaattc atttaaattt acgagtcaag gtggttacat
     7321 aaaagttgta gcttctcaag atgaagagat aacaaccata tcagtagagg ataatggttc
     7381 aggtatagaa aagcaaaatt taaataaagt gttagataaa tttttccaag aagattataa
     7441 taaggctggt agtggattag gactagcaat tagtaatgaa attgtaaaac ttcatggtgg
     7501 aagaatgaaa atagaaagtg aaaagaatgt tggaacaaaa ataactttta atataaaaaa
     7561 taaatttgct aaacaagcat aaattcttag aaagaggatt aagatgagaa acataaaaat
     7621 gatagtagca tacgatggtt caagatataa aggatatcaa aaattaggtg acaataatat
     7681 gactattcaa gaaaaacttg aaaatgtttt aagtaaaatg actaatgaaa cagtggagat
     7741 aataggttct ggaagaactg atatgggagc tcatgcaaga ggacaagtgg taaattttag
     7801 aactaattgt atggattctt tggataaaat tcaaaaatat ttatatgaat atttaccaga
     7861 agatatagtt gtaaaaactg ttgaagaagt tgatgaaaga tttcattcaa gatacaatgt
     7921 aaaatcaaag acttatatgt ataaaattga caataataag tatcataatc cttttataag
     7981 aaaatatgct actcatgtaa gtaaaaaatt agatttagat agaatgagaa aagcaagtga
     8041 gtatttagta ggagaacatg attttactag ttttgcttct tctaaatcta agaaaaaatc
     8101 aaatgtaaga gaaatttatt ctataaatat aaaagaagat gataatgtta tagaaatata
     8161 tgtagaagga aatggatttt tgtataacat ggtaaggata atagtaggtg ctttgataga
     8221 tgttggtcta aaaagaagag ctccacaaga tattaagtat atgcttgaat caaaagatag
     8281 atgtcaatca tcagatactg caccagctaa aggactatgt ctttggaaag taacatatta
     8341 atcaattata taaaactctc tttaaaataa aaataaaata aattatatag ttgacataaa
     8401 tggaaaaata aactattata tattttataa gagagatata aaactttata tttaaatcct
     8461 gtgagaaaga gagtaacttc tatgtgtttt atagagagct gaggatggtg gaagcttggc
     8521 aaataagtat tagtgaaaag aactttggag gatactaatt gaatttagta tgttagtagg
     8581 taatctcgcc ttaagagaaa gagtggataa gtttatctta tcaattagag tggtaacgcg
     8641 gatatatttc gtctcttggt ttttccaaga ggcgtttttt aatatattta attattagtg
     8701 tttatctggt atgttgaaca tttactaatt gattagctaa gtttatcaat aagagtggta
     8761 ccgcggatat ttcgcctctt agtattttaa aatactaagg ggttttttta gtattaaaat
     8821 gattttatgt gagtctaaat tctcagagtc attcaatatt aagaatttct ataaaataaa
     8881 aattataatt taagattggg aggaattaaa aatgaaggaa aaagtagtat tagcatattc
     8941 aggaggactt gacacatcaa tcataatacc ttggttaaag gaaaattatg aagatataga
     9001 tgttatagct gtttgtggaa atgtaggtca ggaagataaa atggaagatg tatatgaaaa
     9061 agctttacaa agtggagcat caaaggcata tgtggatgat ataagtgaag aatttgtaac
     9121 tgaaactata tttaaagctg taaaagcaga ggctaaatat gaaggaaaat acttattagg
     9181 aacttcttta gcaagaccta taatagctaa aaaattagtt gaagttgctc ataaagaagg
     9241 tgcaaaatat atatgccatg gatgtacagg aaaaggtaat gaccaagtta gatttgaagc
     9301 tacaattgca gctcttgacc caactataaa agttattgca ccttggagaa tttgggatat
     9361 aaaatcaaga gaagatgcta tagactatgc tgaaaaacat aatataaagg taacagcaac
     9421 taaagctaaa atatattcag tagatgccaa cctttggcat gtttcaacag aaggtggaga
     9481 tatagagcac ctagaaaatg aacataaaaa agatgtttat aaacaatgtg tagacccaga
     9541 agatgcatgt gatgtagctg aatatgtaga agtttacttt gaaaaaggtg tacctaaaaa
     9601 ggtaaatggc gaagaattat ctccagttgc actgattcat aagttaaatg agttgggttg
     9661 taaacatgga attggtgtta tagatatagt tgagaataga cttgttggta tgaaatcaag
     9721 aggtatatat gagactccag gaggaacaat cctttatgaa gcacataata ttttagaaag
     9781 tgctacttta gacaaagata cattacattt caaacaaatg gtatcttata aatacggaga
     9841 acttatttac aatggactat ggtattgtaa attaagagaa tctatagatg catttatgga
     9901 acaaactcaa gataatgtta caggaacagt aaaagttaaa ctttacaaag gaaatataaa
     9961 acctgctgga atatttactg aaaatgctct ttatgatgaa ggaatatctt cttttggaaa
    10021 tagtgaactt tatgaccaca aagatgcaga aggatttatt aatttattca ctttaccatt
    10081 aaaaatcaga gctatgaaag ctggaaaata ataaaataaa ttatttaaga gtgtataaaa
    10141 acgattttta ggtttttata tgctcttttt ttatttgaat ttattaaatt atacagtaaa
    10201 tagtataaaa aaatatatta tagttccaaa tggcttaata atgtgaaaat ataagaccta
    10261 aaatataaat tttataaata caatagttat tgactctctc attgtaagag ggtttatact
    10321 gaatctaatg aaaatttata gtgaggagat agattatatg agaaaaataa ttgaaattaa
    10381 acatcattca catgattatg agtgtatgtg gaatggtata gaagatttat atataaataa
    10441 aaccggtgaa taccttccaa acgatttttt ctttttactg tcaggttttg gttcattttg
    10501 ttatatgaaa acaaataaag ctgatttgaa aagaatggta gctttgggag atggaaggac
    10561 aaaacagatg tataaatttt tagcaccaat tgttggattt gaatacaaac atcatgagtt
    10621 taaaaagtat gagcaagcat taaagaaagc aaaatctgaa attgatgatg gatatccagt
    10681 tattttagga gctttagata tgtattatct accatattat aagaagctat atcataaaga
    10741 acatattcct tttcattata tattaatggt aggatatgat gatgaaggaa tatatctgta
    10801 tgattgtggt agaacagaat tattacatct ttcttatgat aaattacgtg aaagcatgaa
    10861 ttgcaattcc cctggattaa gcaaagaaaa tactatttgc acaataagaa tgaatacttc
    10921 aaagaataaa tataacattg ctagagaagc tattgctata aagcgtgata tgtttttaaa
    10981 tccacctaaa ggatttttgg gatataaagg ttttgaaaag tttattaaag agttgccaca
    11041 gtggaacact gaattggata aggatgatta tgataagatt ttgaccaata tggttatgtt
    11101 ttttggaaca gtaccaacaa ttccaaatgc aatcaaagga attaaagaac cagatactgt
    11161 tcaatttaaa ggtggatttg ataaaatgag tagaatgctc aacagtattg gggaggaata
    11221 caaaaacaac tcttttatac aagtagctga tatttttgat aaaactgcta ttataattga
    11281 gaaaatatcc aatattataa tagattactt aactcaaaca tgtgatgata cagaacaact
    11341 tccgttattg ttctctgagg tcttagagca tatgaaaaca ggatatttaa tcttggatgt
    11401 ataaaaaata tattgttgac cctaacatta cgtaatagtg taatatgaaa atatagctta
    11461 aatatattag taggtattga tggctgtaaa attaaaattt gaggtgtgga tatgagaaca
    11521 gtgaaacaag tttctgattt aactggaata agtgtgaggg cattacatta ttatgatgaa
    11581 ataggtcttt taaaaccaaa taaaattaca gatgcaggat atagacttta tgatgatgaa
    11641 tctattaaaa ctcttcaaca aattttattt tttaaggaga ttgatattcc tttaagagag
    11701 gttaaggaaa taatgtcaag ccaatatttt gataaggttg aagctctaaa aaatcaaaaa
    11761 aaattactta ttttaaaacg aaagagactg gatgaattga tagagcttat aaatcaaact
    11821 ctaagaggag aaggaaatat tgattttaaa gagtttgata tgagcgagta ttttaatgtt
    11881 ttagaagaat ttaaaagaga gcataggaat aaggtaatta aaatttatgg tagtgtagaa
    11941 aaatacaatg aatatataga aagagttaag tctaatgaag agaaaattgc taaaatggct
    12001 ataaaacaat atggaactat tgagaagttt gctaaagcta taaaaacaaa ttttagcagt
    12061 gatatattga atttaggaga aaaatttgat agatataaaa atgattgttt aaaagaaaaa
    12121 catccaaaat taaaagaatt atatagaaag cttgtagaag atttaagtag gaatccttct
    12181 tcaacagacc ttcaagaaat agcaaaagcg ataacagata tatctaaaaa agattatgaa
    12241 attttctcta tggatactgg tgatgacaac tggtattata tggttcaaaa ttatctagta
    12301 aatcctatgt ggatagaaga agtagataaa aagtatggaa gtggagcagg aaagtttatt
    12361 ggacaagtac taaagactta tctaagagat agaaaaccaa agataaatac attgtatgaa
    12421 aaacttgtag aagatttgag tagagattgt tcttcaaggg aagttcaaag cattgttgaa
    12481 gaaatagaca atgaaatgaa aagaagtaat gaattttata aaatagacaa tggtgaaaga
    12541 tactttgatt atatgtcaga gctttattta caagattcta actatataaa agtaactgat
    12601 aagaattatg gtgatggtgc atctaagttt attggagaag cttttaagat ttattttgat
    12661 aataataatt gttgaaaatt tggaagtaaa aaatatgtaa gttttataaa agcacaaaaa
    12721 taaggatgta gattagctcc atctatatcc ttatttaaag ttattaaaat atatagttta
    12781 catcaaactt aacactttca ataatatttc ccaaatatgt ttttatactt tcaacttctt
    12841 ctaaagattt taatttattt tcagtataaa ttgctttgtc cagaatatta aaccttaatc
    12901 ctatttcatc aaagtactca tgatttaaaa ctttgtacca atggtttttt tctttattcc
    12961 aactcgtaga tattgaatca gcttcttttt gagctttttc ccaatcttca ttttctatag
    13021 atttttctaa aacagatata ttgtctttat atccttctgt aaaatcatat agtttattgt
    13081 taacgtaaaa tccaaataat acaaataaga tagtccatag tataacaaat gttgtagatt
    13141 tcaatattaa tcaccctttt tattttgaat aaatattttg tcattttcat ctaaaacaca
    13201 aataagaaca tctttaaaag tttctatgtg attagatttt aaaataccca ttaaccagtt
    13261 agtatctttt tgagctatgt ctagattttt atttattatt ctaccatcca gtataagtgg
    13321 gataggtaaa tgattaaaag ctcttggagg agtactattg tagcttgatg ctggtacaac
    13381 acttagatta ccatcagtct ccaaaatagc atattgtaca tcttttaggt taaagtatcc
    13441 ttgaatccta agttgttcta ataattcatc tatactaatt ctttcttttt taagctcttt
    13501 atatacaatt tttcctttat ctattaatac agaaggttta cctgataaaa atgaacttaa
    13561 ttttctgctt tttaatgata agaatgaaat tagagtctgc atgattacaa gtccagatat
    13621 tgctgcaaca ccatttatga ttggtatatt attattttcc attgggacag atgcaacttc
    13681 tgcaattaat agtgttataa ctaaatcaaa acagttcatt tcagctattt caccttttcc
    13741 catgactcta agtgcaatta gcactgttat gtataatata atacttctta ttaatactac
    13801 tgtcatatct atcaattact cctttaaaaa atatttacac ttagttagta tatcctatat
    13861 aaaaaattta attcaaagta taaaaaacct taaataattg gattcaacta tttaaggttt
    13921 ttttgataat tttaatatta aattttttat ggattttctg ttacttttat ttattttaat
    13981 aacagattca acaaaactct ttttatctcc taattttaag ataaaatcag gatattcttc
    14041 aaattcattg agttttatta atttgaaaga agaagataga tttttaaagt taataatttt
    14101 agagttattc actaatacac ttcttttttt aaaataaatt tcttttttac ttatatttag
    14161 tatagttaat atttgtttaa gatatattat tatatcttct ggagttctat caaatttgag
    14221 ttcaaaattt tcatcttcaa agtagggaat ataatataaa ggtgttttga gagcaggtaa
    14281 tttattatat aattcttcaa gaatatcgat atttttaggg gagttaaaga aaaagcattt
    14341 acatacattc attaaatcca cagcatctga aagtgagtta tgtttaatat ctccttctac
    14401 agaaaaaatt tttttcatat tcagtaaaga tatttgctta gataagattc tattattgta
    14461 tgtaatatgt tgagatataa aaggttgaat gtcaatcatc atattaaata actctaagca
    14521 tttacctgta tatttataca ctctaaatga agttagaagg ccagttatat caaaatcacc
    14581 ccaattatat attgtaacat catcaaattt acaaaaccat ttataaaaat catccataac
    14641 atataaaaga ttaggagcat tattgatatc tagttggttc aatttggtta aatttttaca
    14701 tttatttgaa agtattttat tgtattttgg cttaactaaa gagtaataat tatctattgt
    14761 attaaattcg ttatcaacta aaactccacc aatagaaatt aattccgcac cattatattc
    14821 ttttggttta tttttattat catctagtat attaaattca aagtctataa ataacttgta
    14881 tttcataaat ctaaagcaca tcctcaggta aattatttat tgagcctaag catcataaga
    14941 atcaatagat ataatgactc taaacatcaa aggttcatta caacttttat ttatttcata
    15001 atttaattca aataaatctt ttaagatata atttataagt attctttccc aacccatttt
    15061 taaattaact ttagagtcta ttaatatatt ttctatcttt ttaaatattt ctatatttgc
    15121 tggtattata ctgagctcaa tattactttt aaacgggtta gactgtaaat ctgttataag
    15181 tatatttttt aattcctctg taagttcaga aattaaacta tcacctatgt atttttttat
    15241 gatttcctct ctaatttctt ttaaatttag caatattagt acctgctact tacatagtag
    15301 cttttccttt ccttttaata ctattattca tttttgaatc ttatttagat atattttaag
    15361 attaaaagtc tttagctata taatttttta cccattttta acttatatta acatattaca
    15421 ggcttatatc tccaccctgt ttacattgag gatggagtat attgcctatt ttgataaaaa
    15481 tgataggttg ctatttgcct attccttttt ctgataattt ttctaacata tctttggtca
    15541 ttttatctaa atcataacta aacttaaagt tccattcttt tactgcacta ctactgtcta
    15601 gagagttagg ccaagaatca gctatacttt gtcttacagg gtctacatca tattctatca
    15661 caaaatcagg aatatatttt tttatagaat cagctatttc ttcaggagaa aagctcatag
    15721 cagtaatatt aaaagcattt ctatggatga gtttatcagc tggagcttcg attaaatcca
    15781 ctattgattg aagtgcatca ggcatataca tcatatccat ttttgtacct tctgctatgt
    15841 aagatttata tctcttattt tttaatgcct cataatatat atcaactgca taatctgtag
    15901 ttcctccacc aggaggcgta acataagaaa taagacctgg aaatctaaca cctctagtgt
    15961 ctactccaaa ttttgagtga taataatcac atagtaattc tccagaaact ttagtcacac
    16021 catacatagt attaggtctt tgtaatgtat cttgaggagt catatttttt ggagaatttt
    16081 caccaaaagc agcaattgaa ctaggtgtaa aaaatttaca atccaattct ttggctattt
    16141 caagaccatt tattaatcca ttcatattta agttccaagc ttctagaggt ttactttcag
    16201 caactgcaga aagtaaagat gctagatgaa ttatagtatc tacatcatat ttttttgcaa
    16261 tttcaaaaaa acgattctta tctgtaacat ctaatatctc aaatatacca gattcacata
    16321 ctggattacc ttcttttact ctccttgaac tagcgattac attttgttct ccatactcat
    16381 ttctaagctt tattgttaat tctgaaccta tttgtcctaa tgcacctgtt ataagtattt
    16441 ttttcaattt tacaacctcc taagggttta atttatgtag tactacttat ttttatttta
    16501 gcaaatcttt tgtttaaata caaagaatta aagataataa tatacgactt aattgaatga
    16561 agtgcagtaa gtgcgtaaaa atttaaagct aatatataat ggtctattgt ataacaataa
    16621 aagagtagat taaattatta tatatagatt gtgtgactaa gtcacagaaa atagagctaa
    16681 tttaatatat aataaaagca tgtcaagtaa agctttaaat taaagtaact ttatataaaa
    16741 agtaatataa attgataaaa ttataaattt atttatagtg aatataggtt ggattttata
    16801 gatacaaaaa tagtatatat gatatgtaga ccattagcat gtttaaagta gcaaaacata
    16861 taaaataaaa tttgaaagga agtataaaaa tgacaagacc atcaatatat catagccaag
    16921 gatacacttg tgcagaggca ttaataaagt cttataatga ggaacacaac acagatatac
    16981 caatctcaat aggaagtggt atgggggttg gaatgaatgt tggaagtgtt tgtggagctg
    17041 taaatgctgc tgttgttata atagggtatt taaatgggcg aaatagtaat ttagatgaaa
    17101 atatagcaag gaagtattca agagagctat tgaatagagt aagaaataaa tacagctcag
    17161 aaatatgtgt atctcttaaa aaaaataaag taacttgtga agaaataata aatttcacat
    17221 atgatgctct aaatgatatg ctaaaaaatc aaaaataatt ctaagaaaca agtattttac
    17281 attcgataga atacttgttt ttttatgata tttttaat
//
LOCUS       DAMJVI010000082        17215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.084, whole genome shotgun sequence.
ACCESSION   DAMJVI010000082 DAMJVI010000000
VERSION     DAMJVI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.084"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(134..658)
                     /locus_tag="PC545_002712"
     CDS             complement(134..658)
                     /locus_tag="PC545_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2457475.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(723..1475)
                     /locus_tag="PC545_002713"
     CDS             complement(723..1475)
                     /locus_tag="PC545_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2457476.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1506..2804)
                     /locus_tag="PC545_002714"
     CDS             complement(1506..2804)
                     /locus_tag="PC545_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2457477.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2821..5085)
                     /locus_tag="PC545_002715"
     CDS             complement(2821..5085)
                     /locus_tag="PC545_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2457478.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5340..6158)
                     /locus_tag="PC545_002716"
     CDS             complement(5340..6158)
                     /locus_tag="PC545_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2457479.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC545_002717"
     CDS             complement(6151..6849)
                     /gene="cbiQ"
                     /locus_tag="PC545_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2457480.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6852..7130)
                     /locus_tag="PC545_002718"
     CDS             complement(6852..7130)
                     /locus_tag="PC545_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2457481.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7120..7872)
                     /locus_tag="PC545_002719"
     CDS             complement(7120..7872)
                     /locus_tag="PC545_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2457482.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8054..8246)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8378..10150)
                     /locus_tag="PC545_002720"
     CDS             complement(8378..10150)
                     /locus_tag="PC545_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2457483.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10164..11093)
                     /locus_tag="PC545_002721"
     CDS             complement(10164..11093)
                     /locus_tag="PC545_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2457484.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11322..11510
                     /locus_tag="PC545_002722"
     CDS             11322..11510
                     /locus_tag="PC545_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457485.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11503..12204
                     /locus_tag="PC545_002723"
     CDS             11503..12204
                     /locus_tag="PC545_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457486.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12206..13129
                     /locus_tag="PC545_002724"
     CDS             12206..13129
                     /locus_tag="PC545_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457487.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13286..14209
                     /locus_tag="PC545_002725"
     CDS             13286..14209
                     /locus_tag="PC545_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457488.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14202..14939
                     /locus_tag="PC545_002726"
     CDS             14202..14939
                     /locus_tag="PC545_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457489.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            14950..15723
                     /locus_tag="PC545_002727"
     CDS             14950..15723
                     /locus_tag="PC545_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457490.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16264..16872)
                     /locus_tag="PC545_002728"
     CDS             complement(16264..16872)
                     /locus_tag="PC545_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2457491.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
     gene            complement(17050..17166)
                     /gene="rrf"
                     /locus_tag="PC545_002729"
     rRNA            complement(17050..17166)
                     /gene="rrf"
                     /locus_tag="PC545_002729"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5545 a         2992 c         2175 g         6503 t
ORIGIN      
        1 tccacaatac aaataaatta aaaagtgaaa tgtatttgtc taaaaacata ataacatttc
       61 actttatcct catttttaag taatatatat ttttaatttt tatttataac taaatctagt
      121 ttttatataa attttagttt ttttctacat ctgagtaatc tattccaaaa gtttctacag
      181 taacagattt cataatttgt ggagtttgtg gcttatcagc tgaatctgtt ctaactgatg
      241 ctatttcatc aactgtatcc ataccttcta tcactctacc aaaacctgca tactgaccat
      301 ctaagtgtgg tgagttttta tgcattatga aaaattgaga accagctgaa ttaggtgcca
      361 tagttctagc cattgatatg actcctcttt catgttttaa attgttagta aatccattac
      421 cactaaattc accttttatt gagtgacctg gaccacccat tcctgtacca tttgggcatc
      481 ctccttgtat cataaatcct ggtattactc tatgaaatat tattccattg taatatcctt
      541 catttactaa tgatacaaag tttttaactg tatttggagc tatatttgga tataactcaa
      601 ttttgatttc ttttccattt tccatttcta tagttactat aggattttta ttttccataa
      661 tttattacct tccttttcat tagttaaatt acataattac atttattata ttatactcta
      721 aattaaagtc tatcaagtat attttttcct atttcatatc cttcttgata aatagtttta
      781 caaaactcat catcaaagtt aagcgtgtct ttaggagcaa attgagtatt aggtctaact
      841 actattattt tatcttcatc atatactttt ttaatatctt ctggcaactc ataattatat
      901 tttgtggata ttaaaataat tttatccaca ttatccccaa ttaatggttc aattaatgca
      961 ctcctaatta aacctccatc taatttgaaa ccatcataaa ttccttcttg cacatctttc
     1021 acaaattgtt ctttaaatcc aagagttgcg tttggatttg atggtaacaa cccactgtat
     1081 gaaatgctga taatggcttc attacgaggc atttttgata catcaacaat cttttctgat
     1141 actactttat tatcaacttc aacataatta acataaaagt tcatttcttc tatattacta
     1201 tcatctagtt cccttaggtt gtctatagca ggagaattat ccacagtatt atttactatt
     1261 tttacatttc cattctggat atcttttata tttgtccaca ttttctccaa gttatcaaca
     1321 ttccctgtgt acaaataata tccatttatt gctcctattg atgtccctga aatcgaataa
     1381 aatttattta ttcctccatc atacaaggct ttaactactc ctgcttgata agcccctttt
     1441 gctcctccac cttctaaaca taatcctaat ttcatagtct catctccaaa ttgtttatct
     1501 tatatttata tttcaaaaaa cttactaaat gttttatata tactatagtg gtcctcaaca
     1561 tttcctaatt ctcttataga atgcattgct aaaattggac ttcccacatc cacagatggt
     1621 atatctatat gagtagaaga tattggacct atagtacttc ctcctctttc atctgaccta
     1681 ttaacaaatt cttgatactc aacattagct tctctacata tatttttata aatagctata
     1741 gaatatccat cactagtata cgcctgacta gcatttatct ttataaccgg accttttccc
     1801 attattggtt tattagttgg gtcatgcttt tctggaatgt ttggatgaat agcatgagct
     1861 aaatctgatg atatcataaa agatgaataa atagactcaa agaatccctc tctatcctta
     1921 cccattgata tacaaattct ctccaatata tttaacaaca tgtttgaatc tgcaccttgt
     1981 ttagtagagc taccaacttc ttcattgtca aatacagcca ttacatttac accatgttga
     2041 cctttagtat taacaagagc attcaaacta gcatgtacca ttgcaagatt atcttgtctc
     2101 ccaatagata taaattcatc attagcccct attaaacttc ctttttcaaa ttcatataaa
     2161 aataaatcaa aatctataat atcgtccata tttacatcta agttagatgc tatttgcttt
     2221 ataagaaaat tatctttttc taatgtatca tttaacatcc caactaaagg taacatatct
     2281 ttttgcttat tatatgaatg accatcattt atacttctat ttaaatgtat tgctagattt
     2341 ggtattatac aaattggctt tttaaagtct ataagccttt catttggttt taatatacta
     2401 tcacctttta aaactactct accagctatc ccaagaggtc tatccaacca tgtacttaat
     2461 attgctcctc catagcattc tgtatttaac ttcaaataag atttttcagc ttctatttct
     2521 gcatttggtt taatactaaa tgtaggagaa tctgaatgag agcctattat tctaaatcca
     2581 tctctttcta tatcttctga atttactaca aaagctacta tagctgataa attcttagta
     2641 acataatact ttccaccaac tcttagattc catctttgat tcaaaactaa ttcttcaaat
     2701 ccatttttat ctaagatttc cttagtactc ttgactgcat gaaaagacga cggactatca
     2761 tatatgaagt ctattaagtt tttagcaaag tttatactat ccattcctca tttacctcca
     2821 ttataaaata tttagtgttg tatattcttt atttattttt ttaattccct gttggtcaaa
     2881 agctatagta acatctgtac caattataga aaccactgtc ccaaccccaa atttaggatg
     2941 atgtacctta gaaccaagtt ttatatcatc tggattagtt tctttattag aatcttttat
     3001 tgtagcatta actttatctg caactttaga tttgcttata gtactcatat actttttagt
     3061 atatttatct aacatattat aacttgcttt agaatagctt agttcttttt ttactttgta
     3121 caatctttct atacattctt ctggcagttc ttccataaat cttgaagcta ttgcaacatt
     3181 tgttttacca tataatgttc tcttctctgt caatgacata tatagctctt ctttagctct
     3241 agttatacca acataacaaa gtcttctctc ttcttctatt tgtgaatcac tcatagattt
     3301 tatagctctt gatataggaa ataacccttc ttccatacct gttaaaaaca caacagggaa
     3361 ctctaaacct ttagatgtat gaattgtcat aagcgaaacc ctatcattgt cttcttcatc
     3421 atttgattca gatgtaagtg ctatacttgt taggaatgtc tctaagtctt tattttcata
     3481 agtatcatca ttactacttt caaactccaa agctatagaa ataaattctc tcaaattatc
     3541 aattctgtct tgagcttctt cacctttgcc tgttaaatct ttttcatttt tatttcttat
     3601 ctctacaagc tcgtccatat atcctgtagt gtccaagact ttttctataa gtttgctaac
     3661 aggataaact tcttttatag ttctaagagt ccctataata tcaacaaatc cacttatact
     3721 tgcccttgcc ttagtagata tatctgaatt tgtttctata tcaattaata ctgagtatat
     3781 actttcctgt tttaagttag ctctatcttc aattttttct atggttctaa gccctatacc
     3841 tcttctagga acatttataa tcctttttat agatatatca tcttgtggat tttgaataac
     3901 tcttaaatac gccaacaaat cctttatttc ttttctctca tagaattttg taccaccata
     3961 tatattgtat ggaatttgac ttctatttaa agcgtcctct acaggtcttg cctgtgcatt
     4021 tgctctataa agtactgcaa aatccttata aggtctattt tcttttctag ctatttgtgc
     4081 aattgaatca gcaatgaaat cagcctcttc tatttcacta ccagttaatt gtatctttat
     4141 aagttctcct tctttttttt cactccaaag cttttttctt tttctttcta tattattaga
     4201 tataacttta ttagctgcat ctaatatcac ttgtgtagac ctataatttt gttccaactt
     4261 tacaacatgt acattgtcat aatctttctc aaattcaagt atgtttctta tatctgcacc
     4321 tctccatcca taaatacttt ggtcatcatc tccaacaaca caaatatttt gatgttctct
     4381 tgctaataat tttatcaatt catattgagc tttacttgta tcttggtact catctaccat
     4441 tatgtatcta aatctacttc tgtaatatgc taacacttca tcattagcct tgaaaagttc
     4501 tacagtttta agtattaaat catcaaagtc aagtgcacta tttcttttta aacggtcttg
     4561 atatagcgca taaatatctg caatcttaac cattctatta tctgccatat tgatatcttt
     4621 aaactgcttt ggagtataaa gtttatcttt tgctcctgaa attgcactta taactgcttt
     4681 tggttcaaac actttatcac ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac
     4741 ttggtctgct gaatcataaa ttacaaaact tctattgtaa ccaatcttat ttatatcttt
     4801 tcttagtata cgaacacagc aagaatggaa tgtacttatc cacatatcct tagtttcagg
     4861 acctatattt tgttcaactc tctctctcat ttcatttgca gctttgtttg taaatgttat
     4921 agctaatata tttggtgctt gtacaccttt atcttctata agatatgcta ttcttgttgt
     4981 taatacctta gttttacctg aaccagcacc agccagtata agtactggac cttcagtctt
     5041 ttctactgct tctctttgag caggatttaa tgtatctaaa ttcatatttc tcctcctgtt
     5101 aacagccata aatatatttt aatctaaaat atgaattact tcaaattata attatgtatt
     5161 atattaatag attatttcca atatattaca attaaataac atttttccat ataatttatt
     5221 tcattgttta ataaaaaaaa taaccttatg atttggggaa tcataaggca cctttaaggg
     5281 gtctaggtta agggttatta tatataaatc tattttttta aaataggttt agggcttaat
     5341 tatttatttg tattaagttc tctttccttc caatacttat acaaatcttc ttctttctta
     5401 aataagggta aattcatatt tctatgtact tttactaacc atggggattc taggccagct
     5461 tgtataatat tattttcatt tataaacaca ttttctgctt ttccttcatc aatcaaaatc
     5521 cccttatcca atacatatat gtaatcacat atttcataca ttaaattcat atcatgactg
     5581 gatattacta tttttatatt atttttattt aatcccttta ttatatctac aatagaccta
     5641 gtacttactg ggtctaaacc agctgtaggt tcatcaagta atacaatttc attttccatt
     5701 gctataactg atgctattgc cactcttttt ttctgtccat agctaagaaa atgtactggc
     5761 ctgtctataa aatctattcc attaacagct actagagctt tgtttattct ctctttaatt
     5821 gttttttcat ccataccaat gtttctcatt gcaaaggcta tatcatcata tacttttgaa
     5881 taaaaaattt gtttttctgg gtcttggaac acaataccta cattttttct aaggttgtat
     5941 aaacttctct tatcatattt taacttttct tctttaaaaa gaatttctcc agatgtaggc
     6001 tttaatatac ccattaaatt cataaataat gttgattttc ctgaaccatt tgaacctatt
     6061 atacctataa catttccctt tgaaaaatcc atattaatgt ttaatagtgc attggtattt
     6121 ttttcatatt gatacgttaa attatttatt ttaaacatca ttatccccta ctatgtgaaa
     6181 tttaccatca tacaatttta tgtctaatga tatacacata tcatcatacc ttgtcataag
     6241 tcttttgtac agcatacttc caagaatacc taaggattta tatgaagttt ttaaatttat
     6301 atatccaaat cttagctctt gtgacttgta tatttctgaa aattcttcta aaaatatgaa
     6361 tataaaccta tatattaaca ttgataactc gacaaaagtg tcaggtaaat gaagtttttt
     6421 aaaaaagaat attaattgat taaaaggtgt tgtaagtata caaaaataca cacaagtcaa
     6481 acaagacatc gctctaaata acacatgaat agacatgtca atagacgcct ttgatattcc
     6541 tacattaaaa ctaaaaaatt caaaactata taataaatct gctttattaa aagatatatt
     6601 tacaagagtt attcctatac taagaaataa aaagtacatt ggtattctaa gtaattttaa
     6661 ataatttttt aaatctattc cagccacaca aacaaccaaa atactcatta aaatcataat
     6721 tccacttaaa ataaatatat tttgtattac catagaagca atcagaaaaa ttactcctat
     6781 ggctactttt ttgtttggat ttactttact caatctattt gtatatgcat atttatcaat
     6841 tataagcatg attaacttct ctttcctttt tgaagaccta atatgtatcc tattacacca
     6901 gcccctaaag ctgcttgtgc agagaataat aaactttcta tttctccact tggtggctca
     6961 tatggactgc taaaccatgg tttgtaattt ggatttatct tagtaatttc actttcagct
     7021 tgaccatctg ctcctccata ttcagcacct gagttgacaa gtaatgggaa tataataaga
     7081 gctactacta ataaaagtaa taatatgtta gtttttgttt tagcactcat tagctttaac
     7141 tcctttatca ttattttcta gtatgctttt ttctccgttt tctgtaatta aattatatac
     7201 aattacagta agtatacctt ctgctattgc tattggtatt tgagtcataa agaaaattcc
     7261 taaaaattta attgctgaag ccattacccc acctgatggg tctgggaaag ccagtgctaa
     7321 ttgtattgaa gttattgtat atgtggctaa gtctcctatt gctgctgcaa gaaatactgg
     7381 aatagcatta tttccatctt ttttctttaa agccttaaat attaggaatg atacgattgg
     7441 ccctattatc gccattgaaa aagcattagc tcctaaagta gttattcctc catgtgctaa
     7501 taacaatgct tggaatatta aaactatagt tccaagaaca aacattacgc ttggtccaaa
     7561 cattattgct ccaagaccta caccagtagg atgtgaacaa cttcctgtta ctgaaggtat
     7621 ttttagtgct gataatacaa acacaaatgc tccacataaa gctaataata ctttcttttt
     7681 agggtcttgc tttactattt tacctattga ttttaatccg aataagaaaa atggtataaa
     7741 tataactccc catgctatag accattttac tggtaagtaa ccttccatta tatgcattgc
     7801 atttgatgta actggagtta aaatgattaa cataagtgca gctataaccc ctaatttaat
     7861 attctgtttc atcttagtat ccctctctcc ctttctaata attaaaacat aataatacat
     7921 gtctgattta tttaataaaa taaaaaagcc taactcgatg gttaggcttc taattttcca
     7981 caatctaaat acaaacacat aaaacaaaca tatctgtata attattataa gaaactttct
     8041 ccatccccga agaaattact ttaatatttt aggcaggtct cctgacttag cttcatccta
     8101 ctcctcttcc ttcccatgag atataaaaaa tatcatcata gtggctaaca aaagtttcgt
     8161 ctgcatcaca gtagcggggg ctgtaaagga atttaacctt tttccctatt aatcttgctt
     8221 aaagaaccta taatattata tatttaattt ttaaattctt cttaaattat tttctatata
     8281 aatcattgta taccttttct gtgaaaattt ctacattttt tataaatttt attcattttt
     8341 atatttttat gtatataaat tcttttttgt aagtttatta atttctaatt atcttcttta
     8401 aatacattga aactacatca gaaaatctag ttatatctct tgaatatata aaatcatttc
     8461 catatattaa tctttcagct tcaagttctg attcttttcc tgtaaatact cctaatacta
     8521 atatattttg ctttctagct tttctcactt cattggctgt atctctcaaa ccaactatac
     8581 ccctataagc cttttctcct cttaatgttc tctcactact ttttcctatc tttacatcat
     8641 ttggtcttcc atcactgaga actatcagga ttttattttc ttcatctcta tttattagac
     8701 cactacatat agattttatt gccaaaccat ctctattatt accagcacaa aaatattcaa
     8761 atatattttc actatttttt atactatcat cataatcttt aaatcttttt aatattgtat
     8821 agtctaaaaa actagaaaat cccatcactc tatttggtat gccaacttct gttagagcat
     8881 ttgcaattat gtaagcttga attgccacat ttccctgatt tctactttga gagccacttg
     8941 catccaataa tatatcaata acgtatttcc ctttttcatt ttctatatct ttataaaata
     9001 ttttattatt attacttcta ccaactctcc aagctctatt ggctaatata gtaccacaat
     9061 cagaatatac tctactttta gcattttctt ctattaaaat tcttgataga ctttctttta
     9121 atctctttat ttgctgtcta tacattctag catgttctct aaattttcct aaattatttt
     9181 ctttttgtct tgttacatat tttagttgaa atacattatt acactcactt ctaagaactc
     9241 catctgtaaa gtgtattcta caaccttcat gaacatttct acaattttgt gtctcaattt
     9301 ttttaatttc acctttactc aaataagttt ttccataata atgttctatt tttgaatata
     9361 tcttatttgc agtgtcttca tcaacatata taactcggtc tgctgaattg ctacatttag
     9421 aatcatcaaa atcccctaca ccttctacaa gcatggatga agatattgaa tctatatcac
     9481 tttcaataat attttcttct tcatcactat aaagttcttc atacatgaaa tcagcaaatg
     9541 tatcaaaatt tacttctaca ctttctacat tattttcatc aataagacta ttttcttctc
     9601 catttaatat gtattcaaaa taagttaaat atataacatc taatgctttt aaaatctcca
     9661 taatatcttg attggtatct atatttctta tatcattaac tattcttcta gtcatactat
     9721 ctaatgctgg gtgcttgtcc atactataaa gtacaaatgt gtatttaatt ttatccataa
     9781 tattatcatt gtgtattttg gtaaaacgtg aaacaatatc tttataagca tttcttctta
     9841 tgtcttctat ccctggccta tctttaatga ccttttcttc aactattgta tttaatataa
     9901 cttgaattag acccagtata atatctttgt tgtatccttt tcttattcta tttgtaagat
     9961 attttttaac tatatcccaa tctacatact ttcttctagc acctgcaatt attgcaaaat
    10021 atagacctat ttcttttgaa gagtaatctt tttctgatat atctatactt tcattgtaat
    10081 caccagctat tgtccatgat aagttattag ctctattttc aaattcataa tcatcatata
    10141 tccatttcat aatctcacct atattaaata tgacttattg gaaacacatc tatactctcc
    10201 catttattag gtattctagt tttaattaca tctccaacca tttctttctc atatacatca
    10261 aaagtctttc ctgtaagtcc catattaatt gcaagagttg gttttaatcc tcttcttata
    10321 gtcttaagag atgccataag accacgtaaa tcaatagctt tactagatat ctctccattt
    10381 tgagacttca attgtaaatc taaaaatatt cctgcaaagt gtattagttt ttcttcatca
    10441 gcatctgaaa attcattttt taaaatcatc attaacttat cttcttctat aggaggaata
    10501 tctattacca taaatctaga taccagtgct tcattaagtt cttttgttcc tgcatattca
    10561 taattcatag tcccaataaa tcttgttgct ggatgtagat tgactctctc atacccaggt
    10621 acatcaatta ttcttctata gtctaaagct gaatgtacca caactatagc atcatttttt
    10681 gccatattaa tttcatcaaa caccccaaaa cctccattaa tagcacactc ataaactgag
    10741 cctcttctaa gtttaacttc attgtctata aatgtatctg tacctataag tgttgaactg
    10801 tctgtattta tatgaaatga tgtattccac tgaggtctat taaatatttc ccctagatta
    10861 tcagcaagta agttttttcc cgttgccttt ggtcccgata ataatatatt ttcttcttct
    10921 aaaatagctg ctatacacat agacaagata tctttaccat aaaaataagt tttagacttt
    10981 gttactctgt attctaaatc tttttctaat ccataataat ttctaaaata cagtacatct
    11041 tcaataagtt tttcatctac cccttgcacc cttagattat ctattatatc catattttta
    11101 ccccttttat ataaatagta tttgtttcat ttcttatcac cattttagca taaaaggata
    11161 taagctaaaa ttgatttttt aatctccccc attttaagta aaaccatgca ccattttatc
    11221 actacaaaat taagagtact acttactgat atttcttgat tttattcgtc tatacgtata
    11281 taatatatac tgataaatta tgaggaaagg atgattgaaa tatggaatat atgtcttgcc
    11341 cagaagtagc aaaaaaatgg ggtatttctg aaagaagagt acaaaaacta tgtgaagaaa
    11401 gtcgcatacc aggagtttca aaactcgggt atatgtggct gataccaaag aacgcagaaa
    11461 agcctgttga tggtagaaca aaaagaagaa aggaagtaca atatgaatag cgttttgatt
    11521 atagatgatg acaaagaact ttgtgctctt atgaaaaaat gtgttgagca agaaaattta
    11581 tctgctatta ctgcacatgg tggtgcagaa ggtctacatt tagtcgataa aaatagacac
    11641 acactctctc ttgtaatttt ggacataatg atgccagata tagacggttt tcaagttctg
    11701 aaaaaaatta gagaaacaag taatattcca gtaattatgc ttactgctaa aaatgacgag
    11761 gatgataaag tttccggact acgtttaggt gctgacgatt acctaacaaa accgttcagc
    11821 ataaacgaac ttatggctcg tgtcaattct cttattcgtc ggtatactat gctaaaccct
    11881 gcttttataa cagacacaga tttcataata ataaatggca tgattattga caagacaaac
    11941 cgaattgttt ctgtcaataa tattcctgtt caacttacaa gcaaagaatt tgatttactt
    12001 gcttttctgg cttctaataa gggacgggtg tttaccaaaa aacagattta tacacaagta
    12061 tgggaagaag aatatgcttt tgacgatagt aacatcatgt ctttcatcag taaattgaga
    12121 aaaaagattg aaccagaccc caaccatcca ttttatattt tgacagttcg tggtgtcggt
    12181 tatcgtttta ataaggaggc ttaacatgaa tacaagtgtc tatctgcttt tgatattggc
    12241 tcttagcctg attattatag cgtaccttgt ttctgtcctg ctacgtgtcc gaatacaact
    12301 tacacttata aaagatgctt tagaggatat aaaaaatgga aaccttaatc gacgtatttt
    12361 aacagatgaa aacgacatta caaaacaaat ttgctatgat attaacgaaa tagcaatcaa
    12421 tagccaattt caacttattc agcaaaaaca atcagaacaa gcctacaaac agctaatgac
    12481 aagtctctcc catgatgtaa aaactccact tgcttctttg gtcggttatt tggaagcagt
    12541 agaaagtaaa attgttgtag gagaggaaaa agacgagtat atccatgttg cgtctaataa
    12601 ggctcattac ttaaagaatt ttgtagaaaa tctatttgaa tgggtaaaat tggattctaa
    12661 ggaacaagtt tttcattttg atatttttga cttgaatgaa ttatcccgta atattatatc
    12721 tgattggata cctgttttag aaagcagtca ttttgaatat gaatttgata taccagaaat
    12781 agagtacttc ttacgtatag acgcaaatgc atatacccgt atcattaata acctattaca
    12841 aaatattatt acccatagta gtggaaacaa aatgacattg agaatttttg aaaacaaaga
    12901 gcaagctcaa attataataa cagataatgg gaaaggtata tcttcagaca atctcccaca
    12961 tatttttgaa aggttgtacc aatgcgacca ttcacgagct tcaaagggaa atggtttagg
    13021 cttggcgatt gcaaaggaac ttattaacgc tcataaggga aatattaccg ccaacagcac
    13081 tcccggtatg ggaacagaat ttacaatatt gcttcctaaa gccctgtaaa agtacgaggc
    13141 tttcttactc taaaacaaga taaaagcaag attatggcaa ggttttggca aggttcatgt
    13201 tttataattc taattgtagt tgattttgac gttgtatcaa ctatcttttg tagaaacaaa
    13261 tttattaaaa gtgaggaaat tatatatgaa tgattttgtg attgaaacaa agcaacttac
    13321 aaaaatttat gggaatcaaa cagtagttga caaagtcaat ctccatgtaa aaaaaggccg
    13381 aatatatggt ctgttaggac gcaacggagc tgggaaaacc acaattatga aaatgatttt
    13441 aggattgact tcaatcacgt atggcgaggt agatgtattt ggagagaata ttaagggacg
    13501 tgaaaaacaa gtatatcccc gtatcggtgc aattatcgaa acgccaggat tttatcctaa
    13561 tctgacagga acggagaact tggagatttt tgcaaaactg cgtggtacag ccgcccctaa
    13621 ttctgtaaaa agagcattgg aaattgtagg cttaccttat aaaaataaaa agctgtttag
    13681 taaatattca cttggcatga aacagcgact tggtattgcc aacgctatcc tgcatgaccc
    13741 agaactgtta gttttagatg agccaaccaa tggtcttgac cctattggta ttgcggagat
    13801 gagaaagttt attagggact tgagcgttga gcatggaaag acaattctta tttccagtca
    13861 tattctttca gaaatctcat tgttggctga tgatattgga attatcgacc acggtgttct
    13921 tttggaagaa agtagtatga gtgaactaga aaagaaaaac agtaaatata tcctgctaca
    13981 agtttcagat attccgaaga catcacttat tttagagcgt caatttaaca cgaaggatta
    14041 ttccgttcaa gatgactaca cgttacacct ttataacact aatctggata tgggtgaaat
    14101 caataaagca cttgtaatgc agaatgtaac tgttattagt tctcaacttt gtaatgatac
    14161 tttagaggac tactttaaaa aaatcacagg aggagagggc attgcttaaa cttattcaaa
    14221 cagaatttct taaactgcgt cgtaggaaac ttatttggct gatgttgttg tctgctctca
    14281 ttatgccgtt tttcgcacta ctatacttca attattttgg taaaacaggt gttgagccaa
    14341 taaagtttta taaatggtct gcatttggtt atacactttg gattattttg cctgttgttt
    14401 tgggtatatt ttgcaccatg cttatacacg aagaaaacca gtatgatatg ctcaaacagc
    14461 tttggattgt acctattagt aaaatgggat acttttttag taaatttttc gttgttttga
    14521 tttactctat ttgttttatg ctgattacag ccgttgcttc tgtgttattt agtgtacttt
    14581 ctggatatgt cgtatttagc tgggcaagca ttttatattt gttgaaaaaa tgtttagaaa
    14641 ttggtgtaat cactgctttt gtaatgttgc cgattttggc aatcgctaca tctcaaaaag
    14701 gatacattct cccaatttgt atcacattgg tatatgcctt ttccggtttt gttataatga
    14761 caataaatat gtatttacac ccattatcca gtatgggagt tattatcatg agaaataaag
    14821 acattccagg tcttatattt tcacaggaaa ttaatgtccc attagcattt ctttgtattt
    14881 gtatttggag tattgtatca gtattgtttg caaatattgc actaggaaga agaaagtgag
    14941 gtgtagataa tgtggaaatt actttgggct gaatgtcaaa aaattcgtcg ttcaaaaatt
    15001 gtttggatta cagtttttgc atccattatg atagcagtca tagtttttgc aggggggcag
    15061 gatgtatata atgacccaga tttacattat ggcttaaaga gcgtttatga cggttcacgg
    15121 tatatagaca atgcaggttg gtatatggac gcagtacaac cttggtcaac tttttttgtt
    15181 cttcctgctg tcattgccct attaggaagc tatattattt gtcgtgaaga agaggaggac
    15241 actattaaat ccctgcgtct tattcccata aatgaaacaa atttaaccat agcaaaaatg
    15301 attattactt ttatattcag tatacttctt tatttgttac tctttactat aacatttttg
    15361 actgaatccg ttttacattt ttccgattta tcgacaaaat tggttttaag ttgcatgaaa
    15421 gagtattttc ttgatggcat aggtgtattc tttgcaattt ctcccattat tgccttggta
    15481 tcgcgcatga aaaaaggata ttggcttgca ttagtattca cagaagttta ttctattgct
    15541 ggactatttg cgggtatgtc aaatgtctta caaacctttt ttcctattgc ggcaatattt
    15601 aatatttctg gatactatat aactactgga gaaaaaatta tggggagtat catgagttta
    15661 ttactttgtg cctgcctttc tgcatttata cttaaagggc taaaacacaa tgaaaaaaat
    15721 tgaaataggt atatcaaaat gaaaaagata aaaaaactga ttattctaat tgtattcttg
    15781 tttctttttg gcatgttaca ctgttatact gaatggaaat tcaacaataa cccacctaaa
    15841 ggggtaatag aaagaataaa tgagtggact gataagattg gactggatta gtaacttatg
    15901 ttaattatag tgaatgtaat attagcaagt tattgtttcg tattttaata ctataacttt
    15961 aaatatttgg ttttaatctt ccaaacgaat ataaaaaaac tgtcacagag gcaacatatt
    16021 tttgaatatt tataaagtgg tagttttgat tttcaaagct accgcctttt cttctatata
    16081 agcaaactta tcctcaacaa agtgtaaaac atggtgcatt ttaagaaaaa agtaccacag
    16141 acacaagcaa taaaagaaac gccgagaacc tacaaaaacg tagtgcttat gcagatttac
    16201 gacgccttct attttctcaa ccgtcttaat gaccttgtac aaaagaaatt tttctaatat
    16261 tttttataat cttatcttgt gatgtttttt atgtataaat gacttaataa caaatccaat
    16321 aaaaattatg gatattggta taattagaac gaggaattta taatcattat aatgtgacat
    16381 tattcttacc caactctcag aaaaaatata tcctatactg attaatgaag tattccatat
    16441 ggttattccc acgatagatg atattacaaa agttataata ttttgacctg ctatacctgc
    16501 tataaaagat atgtatgttc tacataaagg gattaatcta catacacaaa cagaaataga
    16561 agagtattta ttaaaataat cttgagatgc aaatattcct ttctttgcct ttggacatat
    16621 ctctattatt ttatttatga tagatttacc tccccaattt cccactgtat aacaaaatat
    16681 cgaaccaagt atacctgcaa ttatacttaa aattaaaatt gtcagaaatc caaaatgatt
    16741 tcttgcagca attgctccac ataaaggtaa aacaacttcg ctaggtaatg gaaaacaagc
    16801 atactcaatc ataattaata caaatacaga gattaatcca tattgtaaaa taaaactatt
    16861 gattacagac acttacttat ctatctcctt tcttatttaa aggttttaag ggtacatgtt
    16921 ctaaggattc tcaccctata tttaaagaat caatctcttt aaactattat atgtatttac
    16981 acaaagcatt tataacatac tttaaaaata gtaccttaaa ataagaaact taataaataa
    17041 aaaaagatta cgtggctacg tcctactctc ccaggcagtt tcccaccaag taccatcagc
    17101 gctagagagc ttaacttctg tgttcggaat gggaacaggt gtatcctctc tgctattgta
    17161 accacataat ctttatgctt aatatttcca ttaagttttt aataagttta tagaa
//
LOCUS       DAMJVI010000083        16913 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.086, whole genome shotgun sequence.
ACCESSION   DAMJVI010000083 DAMJVI010000000
VERSION     DAMJVI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.086"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            142..990
                     /locus_tag="PC545_002730"
     CDS             142..990
                     /locus_tag="PC545_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDF2457492.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGREEDRTEAQREKEQNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTAEDLAPVPADKIVSIQVCDVLETPYEKLRDESLHDRLA
                     PGEGYGNTEEFVKIIKEHNISPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            1054..2976
                     /locus_tag="PC545_002731"
     CDS             1054..2976
                     /locus_tag="PC545_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2457493.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YLERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKVAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEVHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVPGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPIACLANPILGHEAELKPAEISKEVLVVGGGVGGML
                     AAWVCAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDVVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHEVTVIELREEVGADVISEHRKFLMNDFENYNIHTITGAKVS
                     KFYDDGVDYLLTDGTEHDLKGFDSVVLAMGSRNYDPLSEVIKEVVKETYIVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            3058..3921
                     /gene="aroE"
                     /locus_tag="PC545_002732"
     CDS             3058..3921
                     /gene="aroE"
                     /locus_tag="PC545_002732"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDF2457494.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFKKLDLDYRYLAFDI
                     TVDKVKEALLAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENGVEVKGKKITIMGAGGAATAIQVQCALDGAREISIFNPKDDF
                     YKRAEQTVENIKKDVPECVVNLYDLEDTNKLYEEIQSSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPKKTKMIEDAEANGCKAIGGLGMLLYQGAEAFNLYTGLEM
                     PVEEVNELCFK"
     gene            4121..5338
                     /locus_tag="PC545_002733"
     CDS             4121..5338
                     /locus_tag="PC545_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457495.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYVFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAFLIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5574..7439
                     /locus_tag="PC545_002734"
     CDS             5574..7439
                     /locus_tag="PC545_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HDF2457496.1"
                     /translation="MENVFKRLQEFNGYDGYKESFEMNYLCIYESIPLREQVELANNL
                     VDEILNMYKSESNEIYLLEGSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLNVENLREVVDTFSKSGEYVFYLSNTIKKLEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEDGYKDTKYERLLMNYIISIVDLNEYLE
                     KRDLDKEKINNLARLICNYLLSVEFKYIGNKLELVNRFLPTVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKIEFEKEINGILLSEKWKNIYFEALRDASGKTEDIIKMSEIYDVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLNFFEETFKIDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKIVKEAYEKEKNVILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSIAV
                     AGSRFRNREYLEKELEKSKIYYLTREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7617..8981)
                     /locus_tag="PC545_002735"
     CDS             complement(7617..8981)
                     /locus_tag="PC545_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457497.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDINIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEIDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGIPVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKKQASKIKDKAEVKIPSKF
                     KIIK"
     gene            complement(9160..10503)
                     /locus_tag="PC545_002736"
     CDS             complement(9160..10503)
                     /locus_tag="PC545_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDF2457498.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10576..10677)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11006..11659
                     /locus_tag="PC545_002737"
     CDS             11006..11659
                     /locus_tag="PC545_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDF2457499.1"
                     /translation="MTKIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEV
                     ISDLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKN
                     SKLIGVRFDDCILKNVLFKDVLGKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVF
                     EYTNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            11800..13071
                     /gene="brnQ"
                     /locus_tag="PC545_002738"
     CDS             11800..13071
                     /gene="brnQ"
                     /locus_tag="PC545_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2457500.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAVFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            13273..14460
                     /locus_tag="PC545_002739"
     CDS             13273..14460
                     /locus_tag="PC545_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HDF2457501.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKIIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINTEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNEYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISEKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSVAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(14690..16714)
                     /locus_tag="PC545_002740"
     CDS             complement(14690..16714)
                     /locus_tag="PC545_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457502.1"
                     /translation="MDKKKLILITHDKLNSDHYKEELTNFFGDEIIIETQNILDGIKE
                     NLEGDVVLSLSPLTSNFLIKHFKEDIEIIHGTKALSKLGYEKMMKLPPGTKSLLMTTN
                     KTSAFEMATYLYKIGINHIDFVPTYPDCDEIYDLDTAITPGQIRFIPKYIKNIVDLGW
                     RKISLDTYMSLLVVLKLKNEKFIEKLYKLSKETLSHDFLNTSLDNISKLKTILYMTID
                     EIGDGLIFFNTFNKVTFVNKSLLNMLGLDEKLIKSPSLMEYMPNSFLDKITKNLNIDN
                     MIIYIDEIDKKFILSKKPFYLYKNIEGCLITLKDVNNIEILEQKIRSDSVKRGYVAKY
                     KFNNIIGNSSIIKDCIKKAKKMALTDNPILITGETGTGKEAFTQSIHNHSNRKNKPFV
                     AINCASLPSELLESELFGYEDGSFTGAKKGGKKGLFELAHTGTIFLDEIGDMPHDLQV
                     KLLRVLQEKEIRKIGGTSIIPIDVRILAATNKDLEKLIIENKFRMDLFYRISMFTLDL
                     PPLRKRLEDIPLLLESFLKELPYKNIKLDKSLLEALNSYTWMGNIRELRNCIEYMAYM
                     GSNYLTINDLPQNISSKLNNNHMSSNMSIFNDLNQYDKNICISILKSLHMKPMGRTKL
                     MKFMEYNVTEYEVRNMLEYLTRNGYLISSKGRKGSSITEKGIKIIENNII"
BASE COUNT         6158 a         2079 c         2798 g         5878 t
ORIGIN      
        1 aaatttataa aatctagata aatagaaatc aaaaaatact tttattttta aggtaaaata
       61 atactactag taggataaat aaaaatataa aatcaaaaat actattaatt ataaagaaaa
      121 taaagggggc aaatttaagc tatgaataat caaaaaaatc ttaatattcc aattacaatt
      181 agttcttata cattgggaac agaagtttca ttccgtgata gagttcgtat tgcaaaagaa
      241 gctgggtttg atggtattgg attgcgtgct gaaaattata ttgatgcaaa aaatgcaggt
      301 gttacagatg aagaaatgat tgcaattctt aatgaatatg atatgaaagt tacagaagta
      361 gaatatataa ctcaatgggg aagagaagaa gatagaacgg aagctcaaag agaaaaagag
      421 caaaatgttt atcatatggc tcacttattt aatgttaaac atataaattg tggattatta
      481 gagatgatac cagaagaaca aattataact gcattgggag aattatgtga cagagcaggt
      541 gaacttatta taggtcttga gtttatgcca tatagtggtg taccagattt ggctactgca
      601 tggcgtgttg tgaaaggatg taatcgtgaa aatgcaatgc ttatattaga tacttggcac
      661 tgggctagag caaagcagac agcagaggac cttgcacctg ttccagcaga taagatagta
      721 tcaattcaag tgtgtgatgt tttagaaact ccttatgaaa aacttaggga tgaatcacta
      781 catgaccgtt tggctccagg tgaaggatat ggaaacacag aggaatttgt gaaaataata
      841 aaagaacaca atatatcacc aagagtcata ggggcagaag taatatctga ttctctaata
      901 tctaaagggt tatccactgc tgctgaaact gtatttaatg caactaaaaa agttttggat
      961 aaagcatggt ctgaagtatc tccaaaataa acttatataa tataaaaata tacagagtat
     1021 tataatataa aacttgaaag gaaggaaaac cttatgaaga ttagttctat gtttacacct
     1081 ataagaattg gttcaatgac agttccaaat cgttttgtag ttccccctat gggaaacaat
     1141 tttgcaaaca cagatggtac tttaagtgaa acttctaagg cttattatct tgagcgtgct
     1201 ttaggtggtt ttggattaat aactatagaa tctagtgttg tagacaaaaa agcaaaagga
     1261 ggtcctcgta agccttgttt atatgatgat agcacaatag atagttttaa aaatgtaata
     1321 gatgcttgtc atgatgcagg ttcgaaggta tctatacaat tacaacatgc tggttctgaa
     1381 ggaaatgaaa aagtagcagg acatccatta aaagctgctt ctgcaatacc tgcatcaaat
     1441 ggtcgtaata caccactagc aataacaaca gaagaaatat atgaacttat agaatcttat
     1501 ggagatgctg cattacgtgc acaaaaagct ggtgcagatg ctgttgaagt tcactgcgct
     1561 catggatatc tggttagtag ttttatatca caaagaacaa ataagcgtgt agatgagttt
     1621 ggtggatgtt ttgaaaatag aatgagatta cctcgtctta tcattgaaaa tatacgtaaa
     1681 aaagtaggaa attctctagc aatattatgt cgtataaaca gcactgatga tgttccagga
     1741 ggaattgatg tacacgatag ttcagtaata gcagcatatc tagaagattg tggaatagat
     1801 ggacttcatg tttcacgtag tatacatata catgatgaat atatgtgggc tcctacgact
     1861 ttacatgctg gattcagtgc agaattagtt actgaaataa aaaaagctgt tagtatacca
     1921 gtaataacag ttggacgtta tacagagcca cagtttgctg agttaatggt cagacaagga
     1981 agatgtgatt tagtagcatt cggtagacaa agcttggctg acccagaaat gcctaataaa
     2041 gctaaaaatg gaaaactaga cgaaatgata ccatgtattg catgcttaca aggatgtgta
     2101 ccaaatatgt tccaaggcaa acctatagcc tgtcttgcaa atcctatatt aggacatgaa
     2161 gcagagctta agccagctga aattagcaaa gaagttcttg ttgttggtgg aggtgttgga
     2221 ggtatgcttg cagcatgggt atgtgctaaa agaggacata atgtaacttt agttgaaaaa
     2281 tctgaagttc ttggtgggca aatgcgttta gctgcatatc ctccagggaa aggagatatt
     2341 actaacttag ttagaagtta tatttcaaaa tgtaatcaat atggtgtaaa aatatgtact
     2401 aatacagaag ctacagtaga acttataaag gagaaatcac ctgatgtagt tattatagct
     2461 actggagcaa ctccacttgt acttcctata ccaggaatca atgattcagg attaattcat
     2521 gctgtagacc ttcttgatgg taaaaaatct tgtgggaaaa aagtacttgt tgttggaggt
     2581 ggaatggttg gatgtgaagt ggcagccttc cttggagaac aagaacatga ggttactgtt
     2641 attgagctta gagaagaagt tggtgcagat gtaatttcag aacaccgtaa attccttatg
     2701 aatgattttg aaaattataa tatacatacc attactggtg caaaagtttc taaattttat
     2761 gatgatggtg tcgactattt acttacagat ggcacagagc atgatttaaa aggttttgat
     2821 tcagtagtac ttgctatggg ttctcgtaat tacgacccat taagtgaagt tataaaagaa
     2881 gttgtaaaag aaacttatat tgttggtgat gcagtaaaag ctcgtagggc attagatgca
     2941 acaaaagaag cttttgaagt agcattaaat atataaataa taaataaatt tttctttgta
     3001 aataactgaa taggttattt gcaaagaaaa tgataatatt gagaggagat tatagttatg
     3061 gaaatttcag gtagaacagg attatttgca ttaataggta caccggttgg tcactctaaa
     3121 tcaccagtga tgtataatta tagttttaag aaattagatt tagattatag atatttagcc
     3181 tttgatatca cagtagataa ggtaaaagag gcacttttag caataaaaac ttttaatata
     3241 aaaggagcca atgttactat gccatgtaaa tctgctgtta cagaatatat ggatgaactt
     3301 tctccagcag ctcgtataat tggagcatgt aatacaattg taaatgacaa tggaaagtta
     3361 gttggtcata taacagatgg agtaggctat gttcgtaatt taaaagaaaa tggggtagaa
     3421 gttaaaggta aaaagattac aattatgggt gctggtggtg cagcaactgc tatacaagta
     3481 caatgtgctt tagatggagc aagagaaata tctatcttta atcctaagga tgatttttat
     3541 aaaagagcag agcaaacagt tgagaatata aaaaaagatg taccagaatg tgttgttaat
     3601 ctatatgatt tagaagatac aaacaaactt tatgaggaga tacaatcaag tgatatattg
     3661 acaaatgcaa ctcttattgg tatgaagcct tatgacaatg aaacaaatat aaaagatact
     3721 agtgttttaa gaaaagattt agtagtaaca gatgtcgttt ataatcctaa gaaaacaaaa
     3781 atgattgaag atgctgaagc taatggatgt aaggctattg gtggattagg aatgttatta
     3841 taccaaggag cagaagcttt taatctatat actggattag aaatgcctgt agaagaagtt
     3901 aatgaattat gctttaaata gataagagta atatcttaaa caatgaaaaa acatacgtat
     3961 ttcaatattt ttaataataa attacaaatt gtcatagtgg gttttgcctc atagtccagt
     4021 taaatttgaa gttttaactg actatacttt aaatccatta tggggatgag tttattgtac
     4081 acaaaaatgt taataaatta acaagaatgg gagaaaaatt atgaacaata aaaaatatta
     4141 tcctactgcc atagcgttgt atttttcata ttttttactt ggtattggaa tttcaatttt
     4201 aggacaatac aaaccagaat tttcatcaat gtggggggct aaaacattat cagatggaac
     4261 attggatgta agtattgttt tagctgttat tgctgcacta ggattaggga gacttatatc
     4321 ttatccattt gcaggaccta tttcagataa gtatggaaga aaagtaagtg gtcttatagg
     4381 aaactttcta catgcaattt tctttgttgg aattgtattt tcaccaaatt tttatatagc
     4441 atatgtattt gcaataattg gaggggcagc aaattcattc ttagatacct gtgtaactcc
     4501 ttcttgtatg gagatatttg ctagtttagg aacaatagca aatatgttta ctaaatttac
     4561 aatagcttta gcacaatttt tgcttccttt tataattgga tttgttgcag caaattcaat
     4621 atcatttaaa gttattttta tagtaacagc gattcttatt gtagtagatg cctttttgat
     4681 agctgtgtta ccatttccac cagctaataa tgttattgat aacaaaggca aaacagttaa
     4741 atcagagaag atgaaattta caccaacaag tatagctctt gtttgtattg gatttacatg
     4801 tacatctaca tttgtattat ggttaaattg taatcaggaa ttgggtaaat tgtatgggat
     4861 ggctgaccct acaaaaattc aatcctttta ctcaatggga gtaatatgtg cagtattaat
     4921 aacttcttta cttattaaaa aatatattaa accaataaga atattagtta tatatcctat
     4981 aatagcttta cttatgctat tagttgtata ttttgttcaa actccaacta tttgtatgtt
     5041 agggggattt gtaataggtt attcagcagc aggaggtgtt cttcagttaa caacgtcaac
     5101 tgcaaatgaa atgtttccaa ctaataaagg aaaaataaca tctatagtta tgattgcatc
     5161 tagtatagca aactatgtta ttttaaatat agcaggtatt attacaaaat cagggggagt
     5221 taatggacca aaatatgttg tattgtttaa tgtggcaata acatttgttg gaattttact
     5281 tgcattgttt gtaaacatga gatatgaaaa agaaaaagtg tatgattatg atgtctaata
     5341 ttagtataat atatgcatag agaagtctaa atctaggata gatttttatt aaaagtaatt
     5401 aaaattaata aggatattat ttacagttat aagatagggt atttacataa ttaataataa
     5461 ggatattact agaatttcaa tttctagtaa tatttttttg cgtataaatg taaacttgat
     5521 gaaataagta gtataatcaa tataaattac atcatgtaaa ggaggataat ttggtggaga
     5581 atgtttttaa gcgattgcag gaatttaatg gttatgatgg atacaaagaa tcatttgaaa
     5641 tgaattacct atgtatatat gaaagtatac cgttaagaga acaagtagag cttgcaaata
     5701 atctagtaga tgaaatttta aatatgtata aaagtgaatc taatgaaatt tatttattgg
     5761 agggtagtaa ttctaagtct ttaatttgtt atttcgaaat ttttatgaaa aagataaata
     5821 ctttggtaaa agaaatgatt attgatgaga aatggttata taaattaact aaagaattaa
     5881 tatataagag taaaaaagtt gagtatgtaa aactagggtt agttttaagt gagaaatatc
     5941 taaatgtaga aaacttaaga gaagttgtag atacattttc taaatctgga gaatacgtat
     6001 tttatctgag taatactatt aaaaaacttg aattttacaa tacatattta tttaatttat
     6061 ctaaaaaggc tacaggttca ataaaagtat ttgctattgt taatatggaa aatttagatt
     6121 ctaaaataaa ttcctatcta attgaagatg gttataagga tacaaagtac gaacgattat
     6181 tgatgaacta tattatttca attgtagatt tgaatgaata tcttgaaaaa cgagatttag
     6241 ataaagaaaa gataaataat ttagcacgtc ttatatgcaa ttacttgcta agtgtagaat
     6301 ttaaatatat aggtaataaa ttagaattag tgaatcgatt tttacctact gtagtaaatt
     6361 atgggactaa ctttgaaagt ttatattcaa ttttcctaat agctataaac gtcttaaaag
     6421 atgaaaatat agagtgcaat aagattgaat ttgaaaaaga aataaatggt atcttgctca
     6481 gtgaaaaatg gaaaaatata tattttgaag cattaagaga tgctagtgga aaaacagaag
     6541 atattataaa aatgtcagaa atttatgatg taaacttatc ttttgatgac ttgttgccat
     6601 atctaaatag agatattaga gattttgagg tatattggca tatatctaaa aagggaacga
     6661 caagttctag attaaaatta ttaaattttt ttgaagagac gtttaaaata gatgatttaa
     6721 ttggaaaaat gaaagatata gaaaaagata aattgactca agaatattat gatgatatgt
     6781 tattttttat tgtgcttaaa ggaagtaaat cactatatcc tgaaggaaag aatatatctt
     6841 taaaaggaat atttggaaat ataaatgaag taagaaaaga atccataaat atcttaaaaa
     6901 gatacagaga gaaactatct cttgaagaat taaaaattgt taaagaagcg tatgaaaaag
     6961 aaaaaaatgt tatattgaag gatgaattaa gaagagtatt atatgaaagt aataatttaa
     7021 aaaaagaatt tgtaaatatt gaaaaaataa aagtagatga acatggtaag gatatctatt
     7081 tgacttcaat agctgttgct ggttcaaggt ttagaaatag agaatatctt gaaaaagaat
     7141 tggagaaaag taagatatac tatttaacta gagagaaaga taatctatat gatgaaaaag
     7201 ctattaaaat agttggtgaa actggatatg tgataggcta tgtcccaaga aaagaaaact
     7261 atattttgag caatttatta gatggaggaa aactgctgta ttgtagagta actgaatata
     7321 atttatatga agattgtatc tacgcaaatg tgtacttgag ttataaagat gtaattgaaa
     7381 cagttgaaaa ctctttaaag atggttttag ataagagtag aataaaactt ataaactaag
     7441 acgtttatat attgcttaga tgatgtaatc ctaactgatt ggttattttt tctggaatta
     7501 tggtgtattt tatgagcaat gctaatgaat ataaaataaa aaaacaattg ttttgtatat
     7561 atgatgtata taaatatatt tatatattca tatttacaaa acaattgaaa tttatgttat
     7621 tttataattt taaatttaga tggaatttta acttctgctt tatcttttat tttagaagct
     7681 tgttttttaa ctttatttaa taattcagat tcatctacaa ctaatacttt tctttccttc
     7741 atcaaccatt taccatcaca aatagtagag tctacatttt cactagtcat agaataaact
     7801 atatttccaa ttggattatg cattggtaaa caatgaattg tatctggatt tagaataatc
     7861 atatcagctt tttttcctac ttcaagacta ccaatttctt tttctagtaa agcacatttt
     7921 gctccattta tagtagccat ttctaagact tcttcagctg gtacgacagt tgggtttaaa
     7981 gttcttcctt tatgtattag tgatgtaaga tacatgtccc tcataaggtc cattctatta
     8041 tttgaaggag ctccatcagt accaattgaa acaggtattc ctttttctaa catttcagga
     8101 attgatgcaa accctagaac cactttcata gctgaacctg ggtcatgaga aacttttaca
     8161 tcatgtaatc taaataaatc aatttcctta tgagtaagcc aaactgtatg agcagctaaa
     8221 agatttggtc ctaatacacc tagtttatca aggtattcaa ctgttccaac tccattgttt
     8281 tgctttacaa atgaaatttc atcagctatt tcagcaatat gcatatgtat acctgtattg
     8341 tatttatcag ctagttcttt agttctaaca agtaattctt cagttgtatt aaaaatagtt
     8401 cttaatgcaa accatatttt aattctctca ttagatgtat tattaaactt atggaataag
     8461 tctaattgag tgttgatttc ctcatcagta gttttttgcc atgcctcagg aagaccatct
     8521 cctgtatcca taactgattt agctaaacag gctctaagtc cagttttatc aacagcttca
     8581 accatagcat ctacatattg accaccagat tctaaaaatg tagtgacacc agattttatc
     8641 aattcaacac aacatgcagt agatgaaatc aaggaatctt cataattgaa gctactttcg
     8701 tatggccaaa ttctttctct aagccaagtt aaaagtacaa catcatctgc aagaccacgt
     8761 cctaattgtt gagataagtg aacatgagta ttgactagcc ctggcattag tatttttcct
     8821 ttagcatcat atatctctgc atctttatct acaatattaa tatcaaactt accaacctct
     8881 tttataatat tattttcaat aagtatgctt ccattttcat atacctctct atctgcattc
     8941 atagtaatta gatatgcatt ttttataagt aattgtgaca taaacaccac tccttttcta
     9001 tatattttta tatctatata ttatgataat aaattttcta tacaaagtta atagcaaaat
     9061 caatttacat ataaagtgat tttgctattt ataaatttaa aataaataag attttattct
     9121 atataattta aaatattgac ctagaactat ttaaaaatat taacttagaa ctaagtttac
     9181 aactgcaagt aaacctataa tccaaagtgt aatactgatt ttatcagttt ctttagctac
     9241 tacatgcatt attatatatg aaataaatcc aaagcataaa ccaatactta tactataagt
     9301 taaaggcatc aataaaactg taacaaaagc aggtacagct attttgctat cagcaaaatc
     9361 aatctgtttt atgctttcaa acatgaatac accaaccatt acaagtgcag gagctgcagc
     9421 gtaagtagga actataccaa gtaatggtgt aaatataagt gtagctaaga ataatgctgc
     9481 tgttactatt gaagcaagtc ctgttcttgc acctgctgca ataccagaag cagattctcc
     9541 aaatgttgta acagtactag ttcctaagaa tgagcctatt aaagtagaag aaacatctgc
     9601 atataacatt cttcctaaat cttttatttc accattctca tcttcaagac ccatttgttt
     9661 acaacaagta ataagaaatg ctaatgaatc aaacatgtct ataaacatga aagagaatat
     9721 aggtccaatt aatgagaatt tcaatgcacc tacaatatct aatttaaaag caactggtgc
     9781 tatactagga ggtgtcgaga ttattgctgt tggcaactcc acaaatccaa atataacaga
     9841 tattattgtt gcaactacta tacttatcaa tattccacct ttaacttttt tatattctag
     9901 tattgccatt aagataagag caaaaagagc tattaaaact gttggtgtaa aatctgcaag
     9961 tgaaactata gtatctggat tagctattat aacacccata ttttttaaac caacaaaagc
    10021 aaggaaaaga cctatacctg ctgaagatgc tactgccaat tctctaggaa ttgcgtctgc
    10081 aattgtcttt cgaagacctg ttactgcgag tgtaaagaag acacatccag atagaaaaac
    10141 taccccaaga cctgtttgcc atgggattcc tgcacctata accaaagagt atgtgaagaa
    10201 tgcatttaat cccataccag gagctagagc aaatggtgca tttcctaaaa tcgcaaatat
    10261 aagtgtacca atggctgtag ctagtatggt aactgttata agggcggctt tatccattcc
    10321 agcttcagat aatattgctg gatttacgaa aactatgtat gccattgtta aaaatgtagt
    10381 aactccacct attacctctg ttttaaggct agagccacgt tcgctaaatt taaacatttt
    10441 gtctaaaaag cttaatgatg aattatttcc acctggttgt acattgttag tgtttggtac
    10501 catttttacc tccaaaaaac tttaagatat aaacaaaaaa atatatattc atatagatag
    10561 aatagaacat atattcagag ttgacattct attcatagaa aacccattta aggtgagttt
    10621 gtagaaacta ttagaccgta ttactaatat tatatgaaca ttttctattt gtttttctgt
    10681 tgtattatat cgtaatattg tattatttac aataaatata ataaaattat tcgttttttt
    10741 gtcgatttta taaaaaatta aggttattta ttgaaaaaaa ttactttttc caataattga
    10801 atatgtatga atataatgtc aattattcta acttgtttta tactattaat ggaagtttat
    10861 tataaatgag tttgtgatat aattataaaa gtactgaata catggatatt tcgtaaaaac
    10921 tttttaatat atcaaatatg tatattaagt taatatatta aaaaaaataa tatcaaaaat
    10981 aatataattt atagtggagg ggaaaatgac taaaattatt aaagtgcaaa aacctaaatt
    11041 tacaggagaa ttagaaatta ttgaaaatct tgaagatata cttgaagata tatttaatga
    11101 tgaaaaaata tttaataaaa ttatagaaaa tgaagttata agtgatttag attctgtaag
    11161 agttagcttt gaatcttgta tatttagaaa tgtgtctttt gaaaattgta atttaaaaaa
    11221 gattgattta cttgatgtgg tatttgaaaa ctgcaattta tcaaatacat tttttgatgg
    11281 aggaagtata tacagagttg aatttaaaaa tagtaaattg ataggagtta gatttgatga
    11341 ttgtatactt aaaaacgttt tgttcaagga tgttttaggt aagtattcta acttttcatt
    11401 ctctaaattt aagatagttt ctatcttaga ttcaaatttt gaaaattctg tatttcaaga
    11461 agtaaaaggt gacaatctag tatttgagta tacaaattta aaaaaagcaa actttaatgg
    11521 aactaaactt tctggcatag attttactac taataatatt gagggaattg agattggtat
    11581 agatgatatt agaggagcaa tttttgatgt gtcacaagca attgatttaa cgaaattgat
    11641 gggaattatc ataaaatgaa aaaaatattc ataaaataaa ttgggaaaac gttaacaatg
    11701 tgtgaatttt taccagattg acatttttct gaaaataaag taagattatc ttaatattca
    11761 aaatattttc aaagggggta atttagtgtg agtaagcaga tgagagatat ttttatagtt
    11821 ggttttgctt tattttctat gttctttggt gcaggaaatc ttatgtttcc tccattttta
    11881 gggatggaat ccggcaaaga ctggttaatt cctcttattg gatttgttgt tgcagatgct
    11941 ggtatagcaa tgcttgttat tatagcaaca gcaagatgta agggaagcat ggatgatgta
    12001 cttaggagag ctggtaaagg gttagcaaga gtaatgagtg tagctgctct agcatgttta
    12061 gcacttcttg taataccaag aacttgcgca acaacatatg aaatgggtat tatgcctata
    12121 tttggagacc atggtccagt agctaaagct gtattttcaa taatcttctt tgggctaact
    12181 ttagcattta ctattaggtc ttctaaggtt atagacataa taggtaagta tttaactcca
    12241 gcgttattat tagtattatt tgttttaata gtaaagggta taatttctcc agcaggacct
    12301 atgagtcctg agcatatgat agataaaaat ctatttggtg aaggtatatc acaaggttat
    12361 caaacaatgg acgctctagg tgcagcatct atggcaacta taatgttgat gtctataata
    12421 gctaaaggat atacatcaga taaagaccaa ataggaatga ctattaaagc tggttttgta
    12481 gcctgtgtat ttctagcagt agtatatggt ggtcttactt atttaggtgc aacagtttca
    12541 actttatatg atactagtat acctcaagca tctttattag ttgaaataac tagtatattg
    12601 ttatatggtc aagtaggaaa ggtaatttta ggtgtaattg tggcacttgc ctgtctgact
    12661 acttctacag gtttaacagc atcaatatca acttattttg aaggaataag taaggcgagt
    12721 tataaacaac tggttatagg tatatgtgta gctagtatgc ttatatcaaa tttaggtgtt
    12781 gattctataa tagccatatc agttccaatc ctacaaacta tatatcctgt tttattagca
    12841 atagttgtta tggagttggc tggtaaacat ataaagaatg ataatgcttt taaaggtgca
    12901 gcatatgtta cattagtgat tagtttatta tcagctataa atggaatgac aggagctgtt
    12961 ccatttatac aaagcttgcc acttgcaggt ttgggcttta actgggtaat accagctata
    13021 ttgggtggaa tagttggtaa ctttataaaa agcaacaaac aagtagcata ataatttgta
    13081 attaatttaa ttttataata tattaatttt tagtactatg ggtaggttta atctaaacct
    13141 aatctaccca tagtactttt tagattggat tataatagca tgtatataat tttttataat
    13201 ataatttttc tttgaagtat gataaaatta gtaatagtga taagaatgta caaatttagg
    13261 aggttttaat taatgcttag aaaaataaca gcatttatgc tatccatatg tttaattatt
    13321 ggacttacag gttgtagttt acttgtaggc aagaaagata aagagaaaat tatagatgat
    13381 tttaaagttg cagtggtaac tcaaccatta tctgaaaata aagtacaata caatatggtg
    13441 gaagagatgg caaaagaata tgaggaagag aataaaattg ataaagacaa agatggtcaa
    13501 actaaagtta aacaaactat aaagcatgtt gtattgccag agaattttac ttctaatata
    13561 gacagtgcta ttaataagat agtaaagttg gctgacgata aagaagttca ggctattgta
    13621 gtttcaactg accaagcagg gcttttacct gctttacaaa aggtaaagga aaagcgtcca
    13681 gaaataataa ctatttctgc ccctatggga gatgataaaa accaattaag ccaatttgtt
    13741 gatgtgaatc taggggttag tgcagaggaa agaggaaaag tattagctga acgttcaaaa
    13801 gaaatgggag caaaagcttt tattcattat gcctctacag atgatttgaa agatgttaat
    13861 atagccaaaa gattagaaat gattaaagag acttgcaaaa atataggatt gccatttgta
    13921 caagttaata ctccaaatat aaatactgaa gaagacaaaa acaaagttaa gcagttttta
    13981 aatgaagata ttgaaaaaca agttaaaaaa tatggaaaag atataaatgt atttggtgta
    14041 aatgagtata tggatgaagt tatattgact aaggctttag aattaaagta tatagttgca
    14101 gagcaaagca atccatcacc aatacaaaca tatcctagtg ttatgggtct aaaaatatct
    14161 gaaaaagatg ctcaaaatta tgataaaata aacgacatga taagtgaaaa ggctaaggct
    14221 tttggcatgt caaatagact tggtggatat cctatgccaa tggatgcttt tctaccatca
    14281 gtagcaattt atcttgctac tgaaatggtg aaacaagatt taacacaaga agatgtatgt
    14341 gacccagatt atttagaggc gtttacagaa ttaagatttg gaatcggttc agaatttact
    14401 cctttaacag aggtacttta taattatcaa tcagtaattt taagccaatt aatttattaa
    14461 tattaaggaa gtgtctcaaa atgaactttt tagtttatga ggcactcttt ttctattaaa
    14521 tcaaatatat ttttatcttt ttaacaatta aaaagaagct tatccctata ttgagatagc
    14581 ttcgttttta atataaataa gttttattat tttataaaaa atttatagaa aaataaaaaa
    14641 ttataattat atataaattt tgataaagta aagatgaatt actataggtt taaattatat
    14701 tattttctat tatttttatg cctttctcag ttattgatga gccttttctt cctttactag
    14761 aaattaagta tccattgcgt gtcaaatatt ctagcatatt tcttacttcg tattctgtga
    14821 cattatattc cataaatttc attaactttg tcctccccat tggtttcatg tgcaatgatt
    14881 ttaggataga aatgcatatg tttttgtcat attggtttaa atcattgaat atggacatat
    14941 tacttgacat atgattatta tttaattttg aagatatgtt ttgaggtaaa tcatttattg
    15001 taagataatt ggaacccatg taagccatat attcaataca atttctcagt tctctaatat
    15061 tccccatcca agtgtatgaa tttaaagctt ctaaaagact cttatcgagt tttatgtttt
    15121 tatagggaag ttcttttaaa aatgattcta aaagaagagg tatatcttct aatctctttc
    15181 taagaggagg taaatctaaa gtgaacatac ttatcctata aaataaatcc atcctaaatt
    15241 tattttctat aattaatttc tctaaatctt tatttgtggc agctaatatt ctcacatcaa
    15301 ttggaataat acttgtacca ccaatttttc taatttcttt ttcttgtaag actcttaaaa
    15361 gtttaacctg taaatcatgt ggcatatctc ctatctcatc taaaaatata gtgccagtgt
    15421 gagctaattc gaataatcct ttttttccac ctttttttgc tccagtaaaa gaaccatctt
    15481 catatccaaa tagttcactt tcaagtaatt cacttggtaa tgaagcacaa tttattgcta
    15541 caaaaggttt attttttcta tttgaatgat tgtgtattga ctgagtaaag gcttctttac
    15601 ctgttcctgt ttcacctgtt attaatatag gattatctgt aagagccatt ttttttgcct
    15661 ttttaataca atcttttata atagaactat ttccaataat attattaaat ttatatttag
    15721 ctacataacc tcttttgaca gaatcacttc ttattttttg ttctaatatt tctatattgt
    15781 ttacatcttt caatgtaatt aagcaaccct ctatattttt ataaagataa aatggttttt
    15841 tagaaagtat gaatttttta tctatttcgt ctatgtatat aatcatatta tcgatattta
    15901 agttctttgt aattttatct aagaaactat ttggcatata ttccattaag gaaggtgatt
    15961 ttataagttt ttcatctagt cccaacatat taagtaaaga cttatttaca aaagtaacct
    16021 tgttaaaggt gttgaaaaat attagaccat cacctatttc atcaatggtc atgtaaagta
    16081 tagtttttaa tttagatata ttatctaatg atgtatttaa gaaatcatga cttagtgtct
    16141 ctttggacaa tttgtatagt ttttctataa atttctcatt ttttagtttt aatactacta
    16201 ataatgacat atatgtatct aatgagattt ttctccaacc taaatcaaca atatttttta
    16261 tgtattttgg tataaatcta atctgacctg gtgttatagc tgtatctaaa tcatatattt
    16321 catcacaatc tggatatgtc ggtacaaaat ctatatgatt tataccaatt ttatatagat
    16381 aagtagccat ctcaaatgct gaagttttat tggttgtcat aagtaacgat tttgtacctg
    16441 gaggtagttt catcattttt tcatatccaa gttttgaaag tgctttcgtt ccatgaataa
    16501 tttcaatatc ttccttaaaa tgttttatta aaaaattact agttaaaggt gatagagaaa
    16561 gcactacatc tccctctaaa ttttctttta ttccatcaag tatattttgt gtttctatga
    16621 ttatttcgtc tccaaaaaag tttgttaatt cttctttgta gtggtcacta ttcaatttat
    16681 catgtgttat aaggattaac ttctttttat ccattttatt tctcctaaca aaatagtatt
    16741 tatttttaaa gtatttaaag tataaggcaa gttatattat acaacaaata gtagtataaa
    16801 tttatatatt tgattaaata ataggttttt ttaggatttt atcctataaa aacctatttt
    16861 aaaattattt ttgcaagata aggaaaatag ctttttatat tgtaatgtat att
//
LOCUS       DAMJVI010000084        16585 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.087, whole genome shotgun sequence.
ACCESSION   DAMJVI010000084 DAMJVI010000000
VERSION     DAMJVI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.087"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            156..824
                     /locus_tag="PC545_002741"
     CDS             156..824
                     /locus_tag="PC545_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457503.1"
                     /translation="MSKLESTTEKVEVKWYGYVALILGALFFSGIFKDAPGPLKVLDF
                     NNVLGSFGTLGTINDGVGTLAANFRGDGGTGPRDGWLYALTLIPSVMFALGVVRVIDH
                     LDGMKAAQKLLSPLLKPLLGLPGFAGLTLIASLQSTDAAASMTKELKDDGYIDEKQKA
                     VFCAFQFSGASAITNFFASGAALFPFIGDVPIFIPLALILIMKFVGANLLRLYLNKFD
                     KGEA"
     gene            826..1311
                     /locus_tag="PC545_002742"
     CDS             826..1311
                     /locus_tag="PC545_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDF2457504.1"
                     /translation="MSEVAKKEVKVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVAATAIMTIFISMGGASGVIAGLFTAGQI
                     SQAHVAILLPMLLLSGAQIQFMGRLLGTAELKTKYYPHMFVIATLNGCIAMLIMNYFV
                     V"
     gene            1372..2493
                     /locus_tag="PC545_002743"
     CDS             1372..2493
                     /locus_tag="PC545_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HDF2457505.1"
                     /translation="METNKDYIEYVHKVYDYLHTIPEKGFEEYKTSEFVLKELEKMGF
                     ENINKGIAGTGIIVTIDSKKEGPVFAIRADMDALEFIIDGEKKMIHACGHDGHTSMLL
                     TAAKLIKERNLVKRGKLKILFQPGEEKLYGALRVIDSGLLDDVEEMVGMHLRPGQDCR
                     LGKAIPAMYHCASCIMEFTIKGITCHGSKPHLGVNAVDAVCLAVNAINAIRVNPAIPN
                     SIKVTNISVKGETYNNIPDEAFMALDIRAESNDIIEELKEKAIHAVKTAVSSINAEAV
                     LISKDGVPAAEFDEELVNLAEETIKDVLGADASIGIFKNSGGEDFHYYTQKLKCKTTY
                     IGLGADATPGFHNPNVTFDTKALEYGVDIWCRLVEKRLG"
     gene            2774..3964
                     /locus_tag="PC545_002744"
     CDS             2774..3964
                     /locus_tag="PC545_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2457506.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQKDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIVQKPVYYPFEAKIKSNNRDVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNESEDWLNELNDYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMDRLKTI
                     FENL"
     misc_binding    4098..4353
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4412..5413
                     /locus_tag="PC545_002745"
     CDS             4412..5413
                     /locus_tag="PC545_002745"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDF2457507.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEGIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            5517..5906
                     /locus_tag="PC545_002746"
     CDS             5517..5906
                     /locus_tag="PC545_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDF2457508.1"
                     /translation="MEFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIVDKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDAIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSNIIENVEFSIKDEEE"
     gene            6067..7275
                     /locus_tag="PC545_002747"
     CDS             6067..7275
                     /locus_tag="PC545_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HDF2457509.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFAVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGNKEEQSA"
     gene            complement(7361..8437)
                     /locus_tag="PC545_002748"
     CDS             complement(7361..8437)
                     /locus_tag="PC545_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDF2457510.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSIDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSIEKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTDTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNLDLWNINIIASKMGEIMFSDLDFISNTISLMNTERDY
                     LLKELKNIKSLDIYNTKGNFILCKIKTKELTAKSLREQLLPQKIIIRDCCSFEGLDEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            8666..9193
                     /gene="hpt"
                     /locus_tag="PC545_002749"
     CDS             8666..9193
                     /gene="hpt"
                     /locus_tag="PC545_002749"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDF2457511.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            9282..10214
                     /locus_tag="PC545_002750"
     CDS             9282..10214
                     /locus_tag="PC545_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDF2457512.1"
                     /translation="MDIQLNILLFFQNLRNPILNFIFLVFTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYLHLIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVVF
                     VKIFDYIDDNKNYILLVVLLIPFIIVAIFLNSPEYFEKFGIIVGFVFGYMVEDRFVKF
                     NTDNNNKKINFSNKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSIIFMNFIKNTVVVFYGIAGAPALFKLLKLN"
     gene            10276..11502
                     /locus_tag="PC545_002751"
     CDS             10276..11502
                     /locus_tag="PC545_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2457513.1"
                     /translation="MKKVVIIGGGPAGMIAASTACEKGYDVTLIEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNDDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNDKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIIDTKPSLIGIEVQESFTKDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTILLDLKPALDEEKL
                     DKRVQKDFQKYTNKKFEKALDDLLPKKLIPIIINLSEINPNTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIEEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            11586..11807
                     /locus_tag="PC545_002752"
     CDS             11586..11807
                     /locus_tag="PC545_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2457514.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            11996..12166
                     /locus_tag="PC545_002753"
     CDS             11996..12166
                     /locus_tag="PC545_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457515.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(12259..12720)
                     /locus_tag="PC545_002754"
     CDS             complement(12259..12720)
                     /locus_tag="PC545_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457516.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILNEKIVNETNFKISYRNYIETLINIQNENLSNLRKKSTKTAKCFNISQIT
                     QLKNLLSNTGYNLLIPYKYLTARDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            12903..13370
                     /locus_tag="PC545_002755"
     CDS             12903..13370
                     /locus_tag="PC545_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457517.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSVFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            13413..15176
                     /locus_tag="PC545_002756"
     CDS             13413..15176
                     /locus_tag="PC545_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HDF2457518.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYITEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDMFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYATKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDATYE
                     NNTLSMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            15184..15792
                     /locus_tag="PC545_002757"
     CDS             15184..15792
                     /locus_tag="PC545_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2457519.1"
                     /translation="MDSNDKKGLKSPEIIIINGSPRINKNCSFIANEVKAMLEECGLS
                     CKIFNIYNMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYVLNKPSIDRSKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNNTDDVSYIDNQEFKDIIYKNLKIYIDSI"
     gene            15864..16433
                     /locus_tag="PC545_002758"
     CDS             15864..16433
                     /locus_tag="PC545_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HDF2457520.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
BASE COUNT         6491 a         1680 c         2927 g         5487 t
ORIGIN      
        1 cttgaaaagt aagtaattat aagctattta atcactggaa taaatttaca ataaaaaaat
       61 aaatacaaag gctttatcat taaggtattg gcataagttt tgcttattaa acaagtatac
      121 taataaaatt catttaaaca ggagggagaa tttatatgag taaattagaa tcaacaacag
      181 aaaaagttga ggtaaaatgg tatgggtatg tagcattaat attgggagca ttattttttt
      241 caggaatatt taaagatgca ccaggaccat taaaagtatt ggattttaac aatgtattag
      301 gttcttttgg aacattggga acaattaatg atggtgtagg tacattagct gcaaacttta
      361 gaggtgatgg aggtactggc cctcgagatg gatggctata tgcattgaca ttaataccat
      421 cagttatgtt tgcacttgga gtagttagag ttatagacca cttagatgga atgaaggcag
      481 ctcaaaagct tcttagtcca ctattaaagc cattattagg gttacctgga tttgcaggac
      541 taactttgat agctagttta cagtctacag atgcagctgc atctatgact aaagaactta
      601 aagatgatgg gtatatagat gaaaaacaaa aagctgtatt ttgtgcattt caattttcag
      661 gtgcgtctgc aattacgaat ttctttgctt ctggagcagc actatttcca tttataggag
      721 atgtaccaat atttatacca cttgctttaa tattaattat gaagtttgtt ggggcgaact
      781 tacttagatt atatttaaat aaatttgaca agggggaagc ttaacatgag tgaggtagca
      841 aaaaaagaag taaaagttaa aaaaggaaat gtgatagatg cgtttattgg aggagctaga
      901 aatggattcc aaataagtac aaattcaatg gctccaaatg tcatcttcgg ttttgctata
      961 atttctgtgt tcaatttaac aggtcttttg gatgtaatag ggacaatatt tacacctgta
     1021 atgagtatct ttggacttcc aggtgttgca gcaacagcta taatgacaat atttatatca
     1081 atgggaggag catctggagt aatcgcaggt ttatttacag caggtcaaat aagtcaagca
     1141 catgttgcaa ttctattgcc aatgttattg ctttcaggag cgcagataca atttatgggg
     1201 agacttttag gaacagctga gttaaaaact aaatattatc cacatatgtt tgttatagca
     1261 actttaaatg gttgtattgc aatgcttata atgaattatt tcgtagtata ggtaattata
     1321 ataaagattt aatataaatg gaaagtttaa attttaagga ggagctagat tatggaaact
     1381 aacaaagact atattgagta tgtacataaa gtgtatgatt atctacacac tataccagaa
     1441 aaaggatttg aagaatataa gacatcagag tttgttttaa aagagcttga aaaaatgggg
     1501 tttgaaaata ttaataaagg aattgcagga actggtatta tagtaactat agattctaaa
     1561 aaagaaggac cagtatttgc tataagagca gatatggatg cattggaatt tatcatagat
     1621 ggagaaaaga aaatgataca tgcttgtggt catgatggtc atacttctat gcttttaaca
     1681 gctgcaaagt taataaaaga gagaaattta gtaaaaagag gtaagttaaa aatacttttt
     1741 caaccaggtg aagaaaaatt atatggagca ttaagagtaa ttgattctgg tttgttggat
     1801 gatgtagaag aaatggtagg gatgcattta agacctggac aagattgtag gttgggaaag
     1861 gcgatacctg caatgtacca ttgtgctagc tgtataatgg aatttacgat taaaggtatc
     1921 acttgtcatg gttctaaacc acatttagga gtaaatgcag tagatgctgt ctgtttagca
     1981 gtgaatgcaa taaatgcaat aagagtaaat ccagctatac caaattctat aaaagtaaca
     2041 aatatatcag ttaagggaga aacttataat aatataccag atgaagcatt tatggcttta
     2101 gatataaggg cagaaagtaa tgatataata gaagaattaa aagaaaaagc aatacatgct
     2161 gtaaaaactg ctgtaagttc tataaatgca gaagctgtac taatttccaa agatggagta
     2221 ccagcagcag aatttgatga agaacttgta aacttggctg aagaaactat aaaagatgta
     2281 ttgggagctg atgcatctat aggtatattt aaaaattctg gtggagaaga tttccattat
     2341 tatacacaaa aactaaaatg taaaactaca tatattggat taggagctga tgctacacca
     2401 ggatttcata atccaaatgt aacatttgat acgaaagctt tagaatatgg tgtagatata
     2461 tggtgtagat tggttgaaaa gagattggga taacatccaa actaataatt ctacatttaa
     2521 aattataatt atagaagtta tagaaaaaga ttgcttatgt taattagaaa ataattaata
     2581 tgagcaatct tattttgata taatgaaact gattttggaa tattgaaaaa atatgatgga
     2641 aaagtaatta tattttaaat ctattggaca aaaaagtgat attattataa tattaagaat
     2701 tgtaaaataa taccaaagtg catctagtaa tagacttaac aattataaaa attttattct
     2761 taggagggaa aaaatgagta agtataattt tgacagagta atagatagag taggaacaga
     2821 ttgtgtaaaa tgggatttta gaactaattg ttcaacaaaa gcacaaaaag atggtttgcc
     2881 tttttggatt gctgatatgg attttgaatg tgcagaacca ataatagaag cattacgtaa
     2941 aaaagttgaa cataaaatat ttggatatag ttcaaatgat agtgataaat actttaatgc
     3001 agtttgtgat tggtataaaa ggagatttaa ctgggaaata aatagaaaag atattatttt
     3061 tagtcctggt gttgtacctg ctgttgctat tttagtaaga atattaacta attctaatga
     3121 aggtgtaata gtccaaaaac cagtgtatta cccatttgaa gctaagataa agagcaataa
     3181 tagggatgtt gtaaacaatc ctctgatata tgagaatggt acttatagga tggattatga
     3241 tgatttagaa gaaaaagcta agtgtagcaa caataaagta ctgatacttt gtagtcctca
     3301 caaccctgtt ggaagagttt ggagagaaga tgaattaaaa aaggttgtag aaatatgtaa
     3361 aaaatatgac ctatggataa tagctgatga gatacattct gatttaatta gaaaaggatt
     3421 taaacatact cctttacagt cattatgccc agagtacaag gataaaatag ttacatgtat
     3481 agctccaagt aaaacattta atctggcagg aatgcagttg tctaatataa tagtgaataa
     3541 tgatgaacta aaaaagaaat atcaagaaga ggttacagct gttggagttg caacttcacc
     3601 aaatcccttt gctattgtag ctacaatagc agcatataat gaaagtgaag actggcttaa
     3661 tgaattaaat gattacttag ataataatat acaatttatt gatgaatact taaaagaaaa
     3721 cctaccaaaa gtaaaacttg tttatccaga aggaacatat ctagcatggt tagattttac
     3781 tgcttatgga ttaaatgaag tagaacttga agatttaatg tttaaaaaag ctaatgtatt
     3841 atttgatgaa ggttatatat ttggaaaaga aggaatagga tttgaaagaa taaatgtagc
     3901 ttgtccacag tctttattaa aagaatgtat ggacagatta aagactatat ttgaaaattt
     3961 ataaagtata ggtaaaaatt tataaagtgt ttaaaaataa aaaaataata aacttgacaa
     4021 attttaacta atcacttatc atactattat gaataaatag aaaacataag agagctaaat
     4081 tttattgaat taaactctta aagactttga aaggaagagt agttatagaa tgtaattaaa
     4141 gcgagttggt gatggtgtga gaccaatatg aagtctataa tgaagaacat cctggagttg
     4201 ctaaccgaaa ttttaaaaga gtaggcttag tcggaaccaa accgttatca tattggggtg
     4261 tatcgagtaa tatctgtaca ttgtgagctg ggtttgaatt tatttcaaac taatcagggt
     4321 ggtaccgcgg aaaagcaacc tttcgtccct tttatatagg gatgaagggt ttttttaatg
     4381 gaaaaatata taaatatagg gagtgaatga tatgagcaca ataataccaa aggattataa
     4441 aagtagtttg aacgtaattg atacacaaat agctataaaa aaattaaaag atttttttga
     4501 aatgagacta agtaatgaat taaatcttat aagagtttct tctccgttat ttgtattgcc
     4561 agaaacaggt gcaaatgata atttaaatgg agaaaaggct gtaagctttg atataccatt
     4621 tatgaataaa aatgcagaaa ttgttcaatc acttgcaaaa tggaaaagat tagcccttaa
     4681 aaaatatggg tttgaagttg gtagtggttt atatacagat atgaatgcaa taagaaaaaa
     4741 tgaagaatta gacaatatac attctcttta tgtagaccaa tgggattggg aattggttat
     4801 agataaaagt agtagaaatg aaaagactct aaaagatgtt gtaaaaagat tgtatggagt
     4861 atttaaagat actgaaattt ttgtatgtag tatgtatgaa ggtataaaag aaatattacc
     4921 agaagaaata acttttataa catcacaaga gttagaagat atgtatccag aattatcacc
     4981 aaaggaaaga gaagataaaa ttgtaaaaga gaagaaagca gtatttataa ctcaaatagg
     5041 aaaaacatta atatcaggag aaaaacatga tggaagaagc cctgattatg atgattggga
     5101 attgaatggg gatatattat tttacaatcc tgttttagat tcagcattag aattatcttc
     5161 aatgggaatt agagttgatg aagaatctct tgatagacaa ttgaaacttg ctggatgtga
     5221 agagagaaaa gagtttgact atcataagat gcttttaaat ggcgaacttc cgtatacaat
     5281 tggtggagga attggtcagt caagaatatg tatgtatttc ttacaaaaag ctcatatagg
     5341 agaagtacaa gttggagtat ggccacagga tatgattcaa aattgtagta gtgcaggaat
     5401 tgaattatta taatttaaat ataaactaat tgataaaatt tatatctaat atgttcatca
     5461 aattaattat atcggttata atgtagaata acctataatt ataagaggtg ataaatatgg
     5521 agtttttaaa aataatcatt aatatagttt tagatatttt aaaaaaaatt ttggtcagat
     5581 ttaaaaatgc taaatttggt ttggtttttg tatttgattt acttaaacta cctgatttta
     5641 tgacagataa aagaatcaat atagttgata aaataaaggt aatttctgta ttaattttta
     5701 ctatatccta ctttgtatct ggggttgata taattccaga gatgattgca ggggcatttg
     5761 gttttataga tgatgctata gttttaattt ggagtatagg tatagtgaat gaagaaatta
     5821 ataagtatag agttatcgca aaaaaagata aacattcaaa tataattgaa aatgtagaat
     5881 tttctataaa agatgaagaa gaataacaat ttatatatgt tattcttttt tttattaaca
     5941 aaaaatggtt aaattgttga cggagaggta aaactctatt atcataataa gtggatatcc
     6001 aatatttaac taagtattca atgtgcaaga atgcttaatt taaaaaagtt atggagggta
     6061 aatattatga agaaaaaaat aggtttagtg cccaaactaa tcatcggtat catagtcggt
     6121 attttaatcg gaagttatgc accagaaata atagtacaaa tattagtaac agttagtact
     6181 ttattctcag cattcctaaa atttgttata ccatttataa ttataggttt tgtaacagca
     6241 ggtatagctg atttagcaac aggtgcagga aagttattgg ggataactac gggaatagca
     6301 tatggttcta ccttaatagc aggattatta tcatttgcag tatctacatt aatcttccca
     6361 aattttattg atgcaagtgt tgcaagtcaa attggagacc cagaggcagg aatgttagct
     6421 ccaatattca caattccatt agagcctatg gtagatgtaa cagctgcaat cgtatttgca
     6481 tttgtaatgg gacttggaat atcagcttta agaaatcatg gaaaaggaga gactttattt
     6541 aatttcttcc aagaatttca aggaatcgta acgaaaacgt tgtcaacagt gattatacct
     6601 ttattacctt tatacatagc aggaactttt gcaaatataa ctatagcagg ggaagtatgg
     6661 acaatattag gggtattctg gaaagtatac ttagtagtaa tacctttaca ttttgtatat
     6721 atggcatgtc aattcacagc agcaggtgta ttttcaggaa aaaatccagt aagaatgtta
     6781 aaaaatcaag tacctggata tttaacagct ataggaactc aatcttcagc agcaactata
     6841 ccagttaatg ttggttgtgc agaaaaaaat ggagtaagta agcaaataag agaatttgtt
     6901 gtacctcttt gtgcaacaat acatttatca ggtagtatga tatcaataac aggatttaca
     6961 actgcagttt taatgatgaa tggtatgcca catggaatag gtgtattatt cccttatatt
     7021 gcaatgttag gtatagcaat ggttgcagca ccaggagcac caggtggggc tataatgagt
     7081 gcacttcctt tcctaccaat ggttggaata ccatcagatg gtgggcttgc aagtttaatg
     7141 atagctttat acttaactca agacagtttt ggtacagcat gtaatgtatc aggagataat
     7201 gcaatagcag tagtagtaga taaaataaat gatggaatga gtaaaaaagg aaataaagaa
     7261 gagcaaagtg cttagttttt taataatcaa aaatgagtta tctcaaatag agatagctca
     7321 ttttttatgg tataaaaaat tataaaataa agtgattttt ttatttaaaa attgctttaa
     7381 gagatgatat taataattta ttttcattag gttttaatat acatactcta aagaaatact
     7441 catctagacc ttcaaatgaa caacaatctc ttattattat tttttgtgga agcaattgtt
     7501 ctcgaagaga tttagcagtc aattcctttg tcttgatttt acacaatatg aaattgcctt
     7561 tagtattgta tatatctaag ctttttatat tcttaagctc tttaagcagg taatctcttt
     7621 cagtgttcat taaggaaata gtatttgata taaaatctaa atcactaaac atgatttcac
     7681 ccattttaga agctattatg tttatattcc ataaatctag atttttattt atttcatttt
     7741 tgacattagt gttagatata agaccatatc caagccttat tccaggcgtt gaaaaaaatt
     7801 ttgatgtacc tcttataacg aataaattag aataatcatc aactaattga gtacaagagt
     7861 atgtatctgt atcagtaaac tctacataag tctcatcaat cattaaaaat gagtctgtat
     7921 tttttaaaat ttctctcact tctactttag taaatgcaaa tccagttgga ttactaggat
     7981 tacagattac taccaaatca taatctttag cattgatagt ttttataaga ttttctaaat
     8041 ttatatgaaa attatcttct tcttttgcaa agtatttttc tatagaacaa ttaattttag
     8101 ataactcttt ttcatactct gaataggctg gagagagtaa aagagcctgt ttagggttaa
     8161 ttgtatgtat aaatgatgaa attagttctg tagctccact gccaagtact atattatcta
     8221 tagaacattt gcagtaattg gatatagaag tttttaagtc tatgtaatca gggtcaggat
     8281 acatagatac catatctata ttgtttacaa tgtattgttt ggctaaactt gatgtaccaa
     8341 aaggatttat atttgagcta aaatccataa attcttcctt tgaaaagcca tacttactag
     8401 ataaactata taaattagct ccatggtttg tgctcataaa agtacctcca aaaataaaat
     8461 tatataatcg ataaaaataa ctgattaata tatgtacata tagattataa tacaaaggaa
     8521 tcgttattcc aaatttttaa tggaaaaagc aaaaaagtat ggataagctg ttaatttagg
     8581 tttaaaaatg atgaaaaaga ttttaattga tattttggta tgctataata tataagttaa
     8641 tagaaggaaa gcgggaggat acaaaatgta caaagttaca ggaaaaatgt tgacagaaga
     8701 acaaataaaa gaaaaagttt atgaattagg aaaaaagata gaagaagact ttaaaggcga
     8761 agatttacta gtggtaggta tacttaaagg agctagtgta tttgtttcag atttaataag
     8821 atgtatagat ttggatgtaa atatagattt tatgagtgtt acaagttatg gaaactcaac
     8881 agagtcttct ggaacagtta aaatattaaa agatttagat gtagatattg aaggaaagaa
     8941 tgtgttgatt gtagaggata taatagattc tggtttaaca ttaagtaatc tagttgcagc
     9001 tttaaaaact agaaatccaa aatctttaaa actatgtact ctacttgaca aacctcagag
     9061 aagaaaagct aatatacctg tagactatgt tggatttgtt atagaagaca aatttattgt
     9121 tggttatgga atagactatg ctgaaaagta tagaaattta ccttatatag gtatagttga
     9181 ggatgtagaa tagatttaaa tgtgaatcaa aagcactaat ttctaaggga gttagtgctt
     9241 tttagttaat tatataaaat tttagtaaag gagacatcta tatggatatt cagttaaata
     9301 tattattatt tttccaaaat ttaagaaatc caattttgaa ctttatattt cttgtcttta
     9361 caataagcac agaagcaccg ttattaatat taattacaac aattatatac tggtgtataa
     9421 ataaaaaatg tggacagaaa atactgtttg ctataattgg aaattttgtt gtaaatttag
     9481 gcatcaaaga gtttgtaaaa gctcccagac caataggaat aaaaggtctt gaatcattga
     9541 gagtttctac tgctggtggt tattcttttc caagtgctca tactcaaact gcaacaacat
     9601 tttgggttag tattatgact atatttaaaa agagatatct tcatttaata ggtgcagtta
     9661 tggttttagg agtaggattg tctaggctgt atttagctgt tcattggcca atagatgtga
     9721 tagcgggttg gatattagga atatttttta cagttgtatt tgtaaaaata tttgactata
     9781 ttgatgataa taaaaattat attttacttg tagttttact tataccattt ataatagtgg
     9841 ctatatttct aaatagccct gaatattttg aaaaatttgg tataatagta ggatttgtat
     9901 ttggatatat ggttgaggat agatttgtta aatttaatac agataataat aacaaaaaaa
     9961 taaattttag taataaaaat aaggggagta aaaatataat tttttcaaga atatgtaggt
    10021 ttttggttgg gatagtaaca ataggaatgt tatatattgg cattaagttg tttatttcaa
    10081 tgttaatagt cactttaaat ataagtgact ctactatgag tataattttt atgaatttta
    10141 ttaagaatac agtagttgtt ttttatggaa tagcaggagc tccagcatta tttaaacttt
    10201 taaaattaaa ttgattttaa ggtatagagt caatagttta aattaaaaaa attaagggta
    10261 gaaaagagtg attatttgaa gaaggtagta ataataggtg gtggaccagc agggatgata
    10321 gccgcatcaa ctgcatgtga gaaaggctat gatgtaacat taattgagaa aaatcataaa
    10381 ttaggaaaaa aacttgcaat aacaggaaaa ggtagatgta atattactaa cgcttgtgaa
    10441 atagaggaat taatagaaaa tgtacctaca aatggaaaat ttctttatag tgctttttat
    10501 acttttacaa atgatgatgt aataagtatg tttaataatc ttggagtaaa gactaaaaca
    10561 gaaagaggaa aaagggtttt tccagagagt gataaagcat ttgatattgt aaatgctctt
    10621 gaaagacaac ttaaaagtaa gaaagtaaat atattattaa attcgaaagt agaaaaaata
    10681 atatctaaaa ataataaaat tgaaaaagta attttaaacg ataaaaaaga aattaagtgt
    10741 gattcagttg tagtagcaac tggtggatta agttatccac ttactggttc tactggtgat
    10801 ggatataaat ttgcaatttc acaggggcac acaattatag atacaaaacc atctttaatt
    10861 ggtatagaag tacaagaaag ttttacaaaa gatttagaaa aattatcatt gagaaatgtt
    10921 gaaataaggg tgtttaatag caaacaaaag aaagtttata gtgattttgg agaacttgaa
    10981 tttactaaat ttggtttaga tggacctata atcaagtctg caagttgtag aatgaaagat
    11041 acaaggaaag aaaattacac tatattatta gatttaaaac ctgcattaga tgaggaaaaa
    11101 ttggataaaa gagttcaaaa agattttcag aagtatacaa ataaaaaatt tgaaaaagca
    11161 ttagatgatt tacttccaaa aaaactaata cctataataa taaacttaag tgaaattaat
    11221 ccaaatacgg ttgttcacca aatttctaga gagcagagaa aaaacttagt ccatttatta
    11281 aaaaatttaa agtttacagt aaagagatac agacctattg aagaagctat aataacttct
    11341 ggtggtgtaa aagtaaacga aataaattca agtacaatgg aatcaaaatt agtagaaggt
    11401 ttattttttg ctggagaagt aatagatata gatgcatata caggtggatt caatttgcaa
    11461 atagcttttt ctactggata tttggcaggt tttaattgct aaaaattacc tgaattaaaa
    11521 atgtttaaaa tcacataata ttatcagcaa cacaaaataa attaaacaga aggaaggtaa
    11581 tcaacatggc aagtaacaat aacaacaaca gaacagtagt accaggagca aaagaggctt
    11641 taaaccaaat gaaattagaa atagctaatg aacttggtat gagtaattat caacaagttg
    11701 ataaaggaaa ccttactgca agagaaaatg gttatgtagg tggatacatg actaaaaaat
    11761 tagttgaaat ggctgaacaa caaatggctg gaaaaagcaa cagatagtta taaataatta
    11821 tttataacta ttggctatta gtttagctag agctatctca aatatagaaa gtaatttcta
    11881 aattgagata gctttttttt attaaattat taaatagata tatttttatg taataaattt
    11941 tatgtatttg gataaaataa tacaaatgaa tatgttacat aaggagtgat gtaaaatgag
    12001 tagtgtaaat tttgaggatt taaaaaacaa atttattaat agtgacttag atgaaaaaat
    12061 aagaatatat acaacaacag aaggtttatc agtagaacaa tttaaagagc tactaaaata
    12121 ttatccaata caacatttat ctaaactaga aaaagcactt ggatagcagt atttaatcca
    12181 aaattaaaaa ggcaatttat ttaatattaa aaatattttt tattaaataa attgcctttt
    12241 tataataggt ttaaattatt atgcttttga tatataatta tagattctct catttccaag
    12301 ttcaatagac ccagtattaa taagagtaac tattccttct atatctctag cagttaagta
    12361 tttatatgga attaataaat tataaccagt attacttagt aagtttttta attgggttat
    12421 ttgagagata ttgaaacatt ttgcagtttt agtagatttt tttcttaagt tagataaatt
    12481 ttcattttga atatttatta aagtttctat atagtttcta taagatatct taaagtttgt
    12541 ctcattgact attttttcat taagtatact actgataaaa tcttgagaca atatattatc
    12601 tgcaaattta tctgctgtta attttccaga tgttatgcct ttttgatatt ttctaagaat
    12661 ttttttagta tagcagtata ttttatttct cgtttctaaa tcgagttgtt ctatttgcac
    12721 aaaaccactt ccttctaatt ataaaaatat ttattaaaaa tctatactta actaataaag
    12781 cttcaataac ttatttacaa taaatactaa atcataaaat atttgttatt ttttattatt
    12841 accaagtata aaaaatatta taattgtaaa acttacaaat atatgtaaaa tatttaggag
    12901 tgatgaaaat gttaaatggg ataataaagc ttttttattc tgaaattgta aaaacaaaga
    12961 ttaataactt agattcaagt ggatattctt taagagatga atgtaaagaa atcagaaata
    13021 taactctaaa gatagatagt gtatttttta agcttttaaa gttagctttt ggttcaatat
    13081 tgcttatggt agcttttaat ttaaacatgg ttttaggtat aggagtgttt ttagttggtg
    13141 tagtttatat actatataaa agaaatatgg aagaacaagt aaaagagcat ataaataatg
    13201 tgaaaaataa tatcgacatc tctaaaataa cttttatgga tgaaaaaggg agaacaggtc
    13261 taaatgcatt aattacatta cttcttatag gattattgac aggatttaac tggatattag
    13321 ttgctagttt tgtcgttgtt tttgttttta ctataaagaa tgtatgttaa aatactaata
    13381 aatagtttaa tatatagggg gagaaattga gtatgactaa tggcagttat aaaatgaaga
    13441 atttagtgga aatatccgct atggtgactg aatctacaga tttttttaat ataaaagatg
    13501 acataataga aaaaatgtta gaagttgttc accctacaaa ggcatgtgtg aatctatttt
    13561 ataaaaatga ttctaaatat gcctacttag tatgttcgca aaccttagaa tatgtacctc
    13621 aaatttttcc aataaatagt cttaaaggtg caaaaattga ttttgacaca tatccagagt
    13681 atatacatga ggctgtaaat gaaaaaaaaa taatatatgt ggaaaatgta tttgaagatt
    13741 ctagagcaga aggtgaacga gatttagcaa aagctgaagg ttatattggg agaatagttt
    13801 ttccttttat aataaataac gtagttgttg gatttatgac ttgtttttta aaagaggatg
    13861 attatataac agaacaagat atagatttta tatcatctgt tgcatcactc ttaagtttat
    13921 caatagaaat aactaataaa aataataatg tacaaatttt aattgataaa ctaagaggct
    13981 caataagtgc cataaatgaa gctactaaaa aactatacct taataagagt attgatggat
    14041 ttttggaaaa tttaagtaaa caagcgtgta atattactaa tagtaaagaa gctttaataa
    14101 ttataaatag tcatcataat aaggatgcta tagttagttg ttataatgtt gagcaaaaaa
    14161 agaaaacaaa tttatatcct atgattgata tgtttttaaa gaaagagtca ttgggggggt
    14221 atttaaataa tttaaaacca tgctcaaaaa agggaataaa tttggaaagt tatatatatt
    14281 acaaacttca ggataaaaat gagactatag gatgtatagt ttgtgcaaat agcaaaaact
    14341 atactagtga tgatttaaat attcttagta tattgtccaa acaagtttct gttggtatgc
    14401 aattatatga acataatgaa aaggaagtaa aacacaaagt tcttgaaaac gagttgaaca
    14461 tattaaataa acaacaacag cttattatgg ataaaggtaa aatggattgt aatgatgaga
    14521 aagaacttta tttttaccat aaaccagcaa gggtagtagg tggagatttt tattatgcta
    14581 caaaagtgga tgatgaacat atagtttata ttgttgctga tgtaatggga catggaatgg
    14641 tatcaaatta tgttgttgca atgataaaag gtgcttttaa agtattatgc aatcaataca
    14701 atacaccaag agaaataatg acaaatttaa ataggatgtt atatgatgaa tttgacaaga
    14761 tgggagtatt tacaacttgt ttgataggta ttatagatac taaagaaaat atgattactg
    14821 tgtctaatgc aggtcactat agtcctgtag ttgtaaagaa agatggcaca atagaaaata
    14881 atttaaattg taaaaagggc atacctattg gaataatgga agatgctact tatgaaaata
    14941 atactctttc aatggaagat tatgcaatgg tatgtatgta cactgatgga attttagaga
    15001 taaaaaattc atcaaaagaa gagtatggta taaacagatt agaaaacttt ctaaaagaaa
    15061 attttagatt aaatcaacag ttaatagtag aaaatctaaa attggattta aagaacttct
    15121 catcaaaaga taattatgat gatgatattt taatagttat gttaaaggat aggtagattg
    15181 attatggatt caaacgataa aaaaggactt aaaagtccag agattataat aataaatgga
    15241 agtccaagaa ttaataaaaa ttgcagtttt atagcaaatg aagtaaaagc tatgctagaa
    15301 gagtgtggat taagttgtaa aatatttaat atctacaata tggatattga atattgcaca
    15361 gcatgtggat tttgtgaaaa aacagggtat tgcaagttta atgatgatat gaagccatta
    15421 tataaaatgt ttgatgattc aaaaggaact atagtaataa gtcctgtaca ttttgattca
    15481 atatcagcta aattaaagac tgttgttgat agaactcaag ctatatatgc aagtaaatat
    15541 gtattaaata aaccttcaat tgatagaagt aagaaacgga taggaatgta tattgcagtg
    15601 ggagggagta aatcatatga ttcacagttt aaaggtggac agattgttat ggattttttc
    15661 tttaagagta taaatactaa acttttacac aatatatatt taaacaatac agatgatgta
    15721 tcttatatag ataatcaaga atttaaagat ataatatata aaaatttaaa gatatatatt
    15781 gattcaatat aatttgattt tgtaggtaat attatgtata attgtattgc aataatttaa
    15841 ataattaaaa ggagggaatt tcaatgaaat taccaatagc attatctaat aaacatgttc
    15901 atttgagtca agctgatata gacgtattat ttggagcagg tcatgagtta actaaattta
    15961 aaccattatc tcaaccaggg caatatgcct gtgaagaaaa agtggattta gtaggaccaa
    16021 aaggaacttt aaaaggagtt agagtactag gaccagctag agctcatact caagttgaaa
    16081 tatctttagc agatggtttc ccattaggag taaaagcacc aataaaagaa tctggtgata
    16141 tagctggaac tccaggtgtt aaattagtag gacctgctgg agaagttgaa atgaaagaag
    16201 gagttatagt tgcttctaga catatacata tgagcaatga agatgctgct aaatttggtg
    16261 taactgataa gcaaatagta aaagttaaaa catctggacc aagagcatta gtatttgaaa
    16321 atgtattagt aagagctagt gacagcttta atctagaaat gcatgttgat atggaagagg
    16381 gaaatgcagc aggcgttaaa aatggagatt tagtagaatt aatagttgat taatattata
    16441 aatagacttt atgctagtag atattttatt tgctagcata tttttaaatg taataaattt
    16501 ctataggata aatttaatca attagattaa tttataaaat aaaggggtat tataaaaaat
    16561 gtaataaaaa cttacaaaaa tatga
//
LOCUS       DAMJVI010000085        15787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.089, whole genome shotgun sequence.
ACCESSION   DAMJVI010000085 DAMJVI010000000
VERSION     DAMJVI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.089"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            394..2628
                     /locus_tag="PC545_002759"
     CDS             394..2628
                     /locus_tag="PC545_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2457521.1"
                     /translation="MQNHNYIKMLNKKVEVLSKELDSRNFKLKTLNEIDFFSKIPNRE
                     FMYQHIDDCISKNIHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQNLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELNNMGATIIESARELKIKLPNEKEIYSNVTFSIGSTI
                     WNGEPNMTTLMLFNQVYSALKKAKSEGKNRYILFDFKDNSFYNKLENDKEFLSEISHV
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     HLFEENCKNIRRWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLAELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKEFEKLLSKENNSEKMFIKYSDIGKSLIEKIFDGFFIMQDMKKLRANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEASEHQ
                     KYSNFYFSANCIISENKLLLAKLHLDLIKTAGYINEFESYSIPKMTNTHLDESNILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMFDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLDMIDTNKIEPFLYRFIKRDGQIVTVMYTQCNLSAIDGRSIAQGMYVDVNKIKN
                     LTKI"
     gene            2659..3492
                     /locus_tag="PC545_002760"
     CDS             2659..3492
                     /locus_tag="PC545_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDF2457522.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRIIFINDNLKNLIKSYMQDNPLG
                     MTNIEIAKKLPDNIAKFFTDSHNIQLDWEKNYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKTYIITMANDITKLYEENKRNLHYSITDPLTGAYNRKYLNDRFDIFIGDYIVL
                     IDLDNFKMINDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQLEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKKKFDKND
                     Y"
     gene            3732..5465
                     /locus_tag="PC545_002761"
     CDS             3732..5465
                     /locus_tag="PC545_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2457523.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQDEIPSCDINNDIFETVSEDENRCMVLGRYTVRTKENTQMIL
                     EVNQRCTFEIIEDRGKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDNTYSFFYVNEGLPKILGYTYDEFMEMSGGSAVGAVYPPD
                     LPKALEDCERCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDIENDTLIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLHGNLRETIEIRQVNSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIEEYLCSSDSKNND
                     ALLVIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTHDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSTYE"
     repeat_region   5791..6285
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=5926..5954
                     /rpt_unit_seq="atttacataccacttagttaatataaaac"
     gene            complement(6815..6919)
                     /locus_tag="PC545_002762"
     CDS             complement(6815..6919)
                     /locus_tag="PC545_002762"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDF2457524.1"
                     /translation="MDDYIYYYNNFRYQNRLNSMSPFEFRKYLSTERA"
     gene            7544..7789
                     /locus_tag="PC545_002763"
     CDS             7544..7789
                     /locus_tag="PC545_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457525.1"
                     /translation="MNQKKIKISIKMVIIGIFAVIIAFVLSRLFVEYIDELLRSNWQY
                     QLFESESSYIVFANCIMFSNILLEIFLIYICRKFRKI"
     gene            complement(8542..8688)
                     /locus_tag="PC545_002764"
     CDS             complement(8542..8688)
                     /locus_tag="PC545_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457526.1"
                     /translation="MVFETFDKTIELYPNAKPIFHSDIGFRLDDESFEEYLGRVFRKR
                     CKGI"
     gene            8784..9433
                     /locus_tag="PC545_002765"
                     /pseudo
     CDS             8784..9433
                     /locus_tag="PC545_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
     gene            9974..11329
                     /locus_tag="PC545_002766"
     CDS             9974..11329
                     /locus_tag="PC545_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457527.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISALTNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIVFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATIIASVAVFAICM
                     AFSGPIVSLFIHPEETMLFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(11447..12115)
                     /locus_tag="PC545_002767"
     CDS             complement(11447..12115)
                     /locus_tag="PC545_002767"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HDF2457528.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKNAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLIKDGYGY
                     LEIK"
     gene            complement(12182..12610)
                     /locus_tag="PC545_002768"
     CDS             complement(12182..12610)
                     /locus_tag="PC545_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDF2457529.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGVFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            12717..13310
                     /locus_tag="PC545_002769"
     CDS             12717..13310
                     /locus_tag="PC545_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDF2457530.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENAIKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWVL
                     IIACILLFVFPQKIQSIFSLFLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            13429..14364
                     /locus_tag="PC545_002770"
     CDS             13429..14364
                     /locus_tag="PC545_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDF2457531.1"
                     /translation="MDKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SLGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVIGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMGVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKARNSYQGCKFV"
     gene            complement(14635..15579)
                     /locus_tag="PC545_002771"
     CDS             complement(14635..15579)
                     /locus_tag="PC545_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDF2457532.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLTSEQVAFTRLF
                     FGFIVLGVYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIAGVSIAAVL
                     LYTSPLFLAIFSKICYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGV
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSRPWEILNYAKDLDVLSC
                     MLMLGIVPAALAYIFYAAGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSNKLKQIEKTESI"
BASE COUNT         6005 a         2074 c         1978 g         5730 t
ORIGIN      
        1 atatggtatt aaattatgta taaaaagtta attatgaata aaataattat gcctttgaaa
       61 aatcattaaa cttaaaatta attataaaaa aactctacct tactaactat acagctcaca
      121 atctatctat ttatgaaaaa atatgatata attgcattca aaaaggaaat ctttcaatat
      181 actagaattt aagcattaac taattttatc taatattatt actaaactaa aaaatatttg
      241 tatttgtagc attggagcaa gtaacttaat gtatattcat tttaaatact tatttaaaat
      301 ttagcattta aattatttac aaattaacat ttaaatactt ctagcataaa tacagttgtc
      361 attaaaattt aaagtatctt atggaggaat accatgcaaa atcataatta tataaaaatg
      421 ttaaataaaa aggtagaagt attatctaag gaattagata gtagaaattt taaattaaaa
      481 accttaaatg aaatagattt tttttctaaa ataccaaaca gagaatttat gtaccaacat
      541 attgatgact gtatttcgaa aaatatacat ataggaatca tgttattaaa tatcgataac
      601 tttaaattat ttaatgatat atatggatac ctagtcggag aaaatttatt aattactcta
      661 actcaaaacc tattgcactt tattccagaa tcaaagggaa tagtcgctta tttaaaagga
      721 gaaggttttt taattgtact tccaaatgta tcaaataaag agttaaataa tatgggtgca
      781 actattatag agagcgcccg agaattaaaa ataaaacttc ctaatgaaaa agaaatatac
      841 tccaatgtta catttagtat cggcagcact atctggaatg gtgaaccaaa catgactact
      901 ttgatgcttt tcaaccaagt atatagtgct ttaaaaaagg ctaaaagtga aggtaaaaat
      961 agatacattc tatttgattt taaagataat agtttttaca ataaattaga aaatgataag
     1021 gagttcttat cagaaatatc acatgttcta caaactaaac aaattgaagt attttaccaa
     1081 ccactttata atataaaaaa taatactata gttggatgtg aggcacttgc aagatggaga
     1141 caccctatta aaggacttct tactccagat aaatttattc ctttatttga acagtttggt
     1201 ctgataacgg aattagattt gcatttattt gaagagaact gtaaaaatat aagaagatgg
     1261 attgataaca atgatgtttt tgttccaatt tgttgtaact tttctcgtct tcattttttt
     1321 aaccctaatt ttccaagtat gctaaaaggt attactgaaa agtacagcgt ctctacatca
     1381 aattttagca ttgaaataac agaaaatata ttaatagaaa atacaaatac aattattcaa
     1441 caactagctg aacttcgttc ttatggtttt tctgtatcaa tggacgattt tggttctgga
     1501 tattcatctt ttggtatgtt gcaaaatcta ccttttgata taatcaaaat tgataggata
     1561 tttttatctc ataatttaaa tgattttaaa aatacaacca ttttgtactc tataataaac
     1621 attgcaagag ctcttgggat gcttacagta tgtgaaggtg ttgaaacaga acaacatgta
     1681 gaatttatta aaagtataaa ttgtgatatc gcccaaggtt attattatgc aaaacccatg
     1741 gagtgcaaag aatttgaaaa attactatct aaagagaata attcagagaa aatgtttata
     1801 aaatattctg atataggaaa atctttaata gagaagatat ttgatggatt ttttattatg
     1861 caagatatga aaaaactaag agctaatgtc actgaagaca ttcaatggtt tgatttttac
     1921 aacaaaactg aaattacatc ttttcctaaa gtaatagagc attttgaaaa acatattaaa
     1981 ggtaaaaaat tttctattct ttacaaaagt ataaccccac ataatactga atcagattca
     2041 ttattaatta gtggagaagc cgttttatca gatgaagctt ctgaacatca aaaatatagc
     2101 aatttctact ttagtgcaaa ttgtataatt tcagaaaata aattactctt agcaaaatta
     2161 catttggatt taattaaaac tgctggctat ataaatgaat ttgaatcata ttcaatacct
     2221 aaaatgacaa atacacatct tgatgaaagt aatattttat cacaatttta ttcaatgttg
     2281 cctgtaggta taattcgcta cgatttattt ggtgatatga ttattactta tatgaatgaa
     2341 gaaatgtttg atattatagg ttatacaaaa gaacagtttt ttggtgagat gggcggaaat
     2401 ttaagagcaa ttgttcatcc agatgattta gactttgttt ataaaaaaag tttggatatg
     2461 attgacacaa ataaaattga accattttta tatagattca taaaacgaga tggtcaaatt
     2521 gtcacagtta tgtatacaca atgtaacctt tctgcaattg atggaaggtc gattgcccag
     2581 ggtatgtatg tagatgtaaa taaaataaaa aatttaacaa aaatctagta caaagtattt
     2641 tattttggag aaattaatat gtttaaagaa atttttttaa gaacttttcc aggatttcta
     2701 attataagag attctaacta cagaattata tttataaatg ataatttaaa aaatttaatt
     2761 aaaagttata tgcaagataa tccacttgga atgactaata tagaaatagc taagaaactc
     2821 cctgataata ttgctaaatt ttttacggat tctcacaata ttcaattaga ttgggaaaaa
     2881 aattatcctt atgataaaat ttcaaattgg attttagagt ttaaaaaaga tactacaagc
     2941 tattggaatg ttttagaata taaagtagat gttgatgaaa aaacctacat aattactatg
     3001 gccaatgata taacaaaact ctatgaagaa aataagagaa atcttcacta ctcaattact
     3061 gacccattaa ctggagcata taatagaaaa tatttaaatg atagatttga catatttata
     3121 ggagattata ttgtactaat tgacttagat aatttcaaaa tgataaatga ctatgaaggt
     3181 cataatgtag gagacaagat tttatgtgat tttgtttcac ttttaaataa agaattaata
     3241 aattctactt ctattataag attaggagga gatgaattta ttgttatatt ctctagtgat
     3301 gttgacaaaa attatgttta ttcacaacta gaagccttaa gagaaaattt tttaaaagta
     3361 ttctctaagt ataagtatct ttcatttagt tatggtgttg atacagtaaa aagaaattta
     3421 aaattgacaa tagttgaatt ggataaaaaa atgtataaaa acaagaaaaa atttgataaa
     3481 aatgattatt aatattgtta ttttaaaata aattaaaatt atctttcttt ttttaaactt
     3541 gtacattagt ttataaatat gtatctatac aagatattga cattaagtac tgaagcatat
     3601 attgtttcag ttcttttttg ttaaaattaa cttatttaac aaagatatca tagtttaaca
     3661 aagcttttaa tttaaatata ttgatgtata atataattaa aatataatag caaaaagaaa
     3721 ggaggaatat catgagcata ctcctaaaaa aagcacccaa attagcaaaa catattataa
     3781 caagttttta tataaatcgg gatattgatg aagtattaaa atacctttgt gaaaatgtta
     3841 catggatcgg accaggagaa caggaatttc tcacttcttt taatgaaata aaaaattatt
     3901 tttatgcagg tcaagatgaa attccttctt gtgatataaa taatgatata tttgaaactg
     3961 taagtgaaga tgagaataga tgtatggtat taggaagata tactgttcga actaaagaaa
     4021 atactcaaat gattttagag gtaaatcaac gttgtacatt tgagataatt gaggatagag
     4081 gaaaattatt agtaaagcac atgcacatat caaaccctta tggtgaaatg caacttgatg
     4141 aatactttcc cactaaaatt ggaactcaaa gttatgatta tttgcaaaga ttactaaagg
     4201 aaaaaacaga agttattgaa atgattacaa ataatatcaa tggtggctta aaaggtagca
     4261 atgatgataa tacatactca tttttctatg tcaatgaagg attgcccaaa atattaggat
     4321 acacatatga tgagttcatg gaaatgagtg gtggttcagc agtgggtgct gtatatccac
     4381 cagacttacc taaagcacta gaagattgtg aacgatgttt tgcaaaagga cctacatatt
     4441 catctgaata tagaatccga aaaaaagatg gaacattaat gtgggtatta gattctggaa
     4501 tgaaatcatt aaatagtgat ggcatagtaa aaattaatag tattattact gacataaccc
     4561 aactaaaaaa catagaatct gaactaaaat tagaaagaga gcgatatcgt attgctttgc
     4621 aaaatattac agatatcatg tttgagtatg atattgaaaa cgataccctt ataaaatatc
     4681 aacgtgtaga aattgacaaa aaaattgaat tagaaaattt tgaaacaaaa aattactcca
     4741 aactattaga atctggtaaa attattcacc ttgatgatat tggaaaacta ctagaagtac
     4801 ttcatggaaa tcttcgtgaa acaattgaga taagacaagt aaattcatta actaaaaatg
     4861 aatggagatg gattcgtgtt caatgttcag ttatatatga tagtgaccat aaccctatca
     4921 aaacaatagg cgttttaaaa gacataactg aagataaatc taaattagaa agcttaatta
     4981 atcaggctca acgtgaccca ctaacacaat tatataatca aagagtatca caaaatctta
     5041 ttgaagagta tctatgtagt tctgattcca aaaataatga tgctctatta gttattgata
     5101 ttgatgattt taaaactgta aatgatactt ttgggcattt agagggaaat gaagttttag
     5161 ttgctgtatc aaaaatacta ttacataaca cacatgataa agacatagtt gctcgaattg
     5221 gtggagatga atttacaata tttataaaat cattaacaaa ggatttaata ataaaaatta
     5281 ctaatgatat tttaaatgat gcaagtaaaa taaaagtaaa ggacaatcat aaaataacat
     5341 taagtattgg tattgccttc actgatgatt caactaaatt atataaagat ttattttcta
     5401 aagctgataa agctctttat ctatcaaaag cagatggcaa aaattgttat agtacatacg
     5461 aataataaag ctatttatac gaccaagaat gaacctttaa atttaatttc atagtctaac
     5521 aatcaaagag ttgaagctaa tcttatagct agtcaactag ttcctagaaa aaatttaaac
     5581 aatatattta ttgatactgc tcatttttct aaagagcaaa tttatataaa aaattaaact
     5641 tttatgtaca tacagatact aatgataaac agttatcatt agtatcttct atcttaaaac
     5701 aaaatgataa tttatctata taaaaaagtg ctttgttgac agagttcact tttttatata
     5761 ggtacagaat ttaagtataa atacatgttt tattctatac attttagtta atgtaaaatt
     5821 tcttgattgt actctctaac ttttcaacat tatactattt atataccact tagttaatat
     5881 aaacccctta gctggttcta gtgctttttc tatgatttta gtcatattta cataccactt
     5941 agttaatata aaaccaattt agatgatgta tatgtgcaaa aggaagaaat aatttacata
     6001 ccacttagtt aatataaaac gctactaaac ttgatataca tgaccattat aataataaat
     6061 ttacatacca cttagttaat ataaaacttc cataaaaaca tccccttttt attcttcttc
     6121 cttatattta cataccactt agttaatata aaacggtcac tacaacttat acttccagtc
     6181 taacgatttg aatttacata ccacttagtt aatataaaac atattttgga tttattaata
     6241 taaacataat taaaaaattt acataccact tagttaatat aaaaccccaa aataaactgg
     6301 gcattttcaa tacttacaca tatgcaactc tcttaaattt gcagtgaacc atgagtagtg
     6361 caaatgataa agcttatcat ataccctcaa caccttatat ttcaagcgtt aagctctact
     6421 ttgtaacaaa tatcgctcac tgcaaaacta tttacatttt tattatatca taaatatatt
     6481 atttttgaat atctatacca atttgtggta taataaaagc aagaagaact acaatctatt
     6541 tagacgtaga gtgaagttca aatactaatg tttattcttt ttgaacttga tttttagttc
     6601 aaaactaaag tcactcctgc ctgtgtgaac tatacccgct gttttataca gttaaaacat
     6661 gtaaaaatag ttataaagta ctagtgtaac tcattagtac tctttttatt atattcatat
     6721 aattaatatc tatattttaa agtttactat ctaaaaaaga gcacgtcaaa taaaacccta
     6781 gatatttaat ttttttagag atacttaaaa taaattatgc tctctctgta cttaaatatt
     6841 tcctaaattc aaatggagac atagaattta atctattttg atatctaaaa ttattatagt
     6901 agtatatata atcatctatt gctttaacta attattcata tgtatcaaat ttccttaaat
     6961 agtacatttc tacctttaaa attccccaaa atccttccat aggagcattg tcaaggcaac
     7021 gaccaactct tgtataatat atgtatatct aacaccatta agaaatatat attttataat
     7081 atatatttct tcatttaatg cacttagaaa agcaaagaaa atacagatta gtacactttt
     7141 tagagaaaaa taaaattaat acatctaaaa tattaaaaaa tactttagat aatctaaaga
     7201 cacatctcaa agtttttaac taaattataa aactaattac aaatgctatg taacgataat
     7261 aaataaaaaa tttacttcgc ttaagcgacg tgtcgttgaa atatttcatg ttgccaactg
     7321 gttcgtaagt tgataagaaa agaacactaa ttatttagag ttcttttctt agctacaagt
     7381 ggattataat acttttaaat ctgtgagaaa atcatatata ataatgatat gaacaagtaa
     7441 agaaaataca aaaatgacaa aattataaat aaaatattat aaaatcataa ggtcataatt
     7501 gttatattgt agaaacaaag taaagagaga gggtggagaa taaatgaatc aaaaaaaaat
     7561 aaagattagt attaaaatgg taattatagg tatatttgct gtaattattg cttttgtatt
     7621 atccagatta ttcgttgagt atattgatga attattaagg tctaattggc aatatcagct
     7681 atttgaaagt gagagttcat atatagtatt tgccaattgt attatgttct cgaatatatt
     7741 attagaaata tttttaatat atatttgtag aaaatttaga aaaatctaat atggtcaacg
     7801 tatttcgaat cgaatacttc atgattattt agtctattgt gctagttaaa gtttaattcc
     7861 catataaagt aaattgattt tagtaaaaca aatcacccct aagggttagt ttatggataa
     7921 gtcatccaaa tattaactct ttaatttttg ctatagattc atctttgctc ataactttac
     7981 ttataaaaaa tgaaatatta taagctagaa cttttattct aattctagtc ataaatccta
     8041 gcatagagtt acttttttta tattataaat aaaactatac aacaaagata atatttagta
     8101 aaaataactt tcattttaac taaacttaaa attcaattac tctctaaatt gacgtttagg
     8161 aattagttca aaataaaaaa gagtgtatat attgaaatta ctacatttca atatatacac
     8221 tctttttaaa tttctcagtt ttccatggaa ctttacttta tttatttctg tattagttgt
     8281 attttttatg catttttctt taaaatcata ttttctgatt caactcttat attataaaat
     8341 ttctttacta aattattctc tttttctata tcttcatata tattttttaa tcgcctttta
     8401 cctaatcttt tatctaacat tactaatgat ttaactaata tattatatga ttttatcgca
     8461 tcttctattg agatatttaa acatatattt actgcttcat aaaaatcaaa ttcattaaaa
     8521 aatccatttt tatttacaat cttatatccc tttgcatctc tttctaaata ctcttcctaa
     8581 atactcttca aaactttcat catctaatct aaatcctata tcactatgaa aaataggttt
     8641 tgcatttgga tatagttcta ttgttttatc aaaagtttcg aataccaagt tattattatt
     8701 cgattttcca acagtccact ataaacaata ataagtttgt tgctccaaat aatgtaacac
     8761 ttgtagaaag atgttacaga tatataaaag atagagaatg tagtttttct cctaatacta
     8821 ttagaaatag aaaaaatatg attaaaaatc aaattgaagt tttctttaaa gatatgaaat
     8881 taatagatat aactccttct attttacagt cttatataaa taacatatac aatgagcaca
     8941 tgttaaattc aacaaaaaac caagtcgatt ttataaaatc agtattgaaa gaatcttata
     9001 gattaaaaga aatatcagaa aatatttgtg attttgttac aaccccaatc aaaaaaaata
     9061 gttcaacttc taagctttat acaaaacaaa aagctcaatt attattagaa aaatcaaaaa
     9121 atattcctat aggtatacca atttttttga tgctaactct tggattacgt tttggtgaag
     9181 ctgtatcact tatatggtca gatgtagacc ttgataaaaa atctcacatg taaatcaaac
     9241 attagtttat gtatacaata aaataatctt caaaggccct aaaaccccta gattcaaaag
     9301 aagattattt gttcctgatg aacttataag tttgtttaaa ggggaaaaat taaaacaaaa
     9361 taagttaaaa ttacagggta tattgaaaaa tgaatttgat ttgatatgct taaataaaag
     9421 attcaacccg tgagttcaac caacttttgt tgttcatttt aaaaaactat taagagataa
     9481 taatcctaga gatataaaag tacatgaatt aatacatgca aatgcgactt tgatattact
     9541 ttcaggtact aacataaaag ctatttataa aatattagta catgcagata taaaaatagc
     9601 tatgaatata tattccatat tctcacttat tagatgaaat gaataaagaa acatctgaaa
     9661 atataagcaa aatactattc aaataatgtt cagttttatg tcaattaaat gtcactcaaa
     9721 gtgctttttt tccatttgtc agttaaatgt cacttaaagt acctttttcc tatttgtcag
     9781 ttaaatgtca gtaatatata aataagatat atttataaga cccttaaaat cactattatt
     9841 taataaaatt tataattata tactatttgc tgttgacatt ggtattatac catagtttat
     9901 catttatatt agaataaaat tattaattaa tagtttcgta attttataat agatttacag
     9961 ggagtgataa taaatggata caaaaatatc tataaacgaa aacatgagtc taggtaaaag
    10021 attttttaaa tacctagcac catctgtagt tgcaatgtgg gttttctcac tatatacaat
    10081 ggttgatgga atatttgtaa gtaaaggagt tggagaatta gctttagctg ctgtcaatat
    10141 atcaatgcct tttattaatt ttatatttgc agtttctcta ttattttcaa ctggtgcatc
    10201 taccataatt gcaatctatc tggggaaaaa agatattaaa tctgctaacg aagtcttttc
    10261 atttaactta gtatcaataa ttatattatc aataattata ttagctataa ctttttttaa
    10321 tttagataga ttagcactat ttttaggagc tacagaatct acaattggta tggttaaaga
    10381 ttatttagga ataataatat tttttaacgg attctttata gtgtcttatt ctttagaagt
    10441 tataataaaa actgatggct ttccaatatt agctactgtt ggtgtaataa tatctgctct
    10501 tacaaacatc atacttgatt acttgtttgt aattgaattt ggatggggtg taaaaggtgc
    10561 tggtattgca actggtttgt ctcaagtctt ttctactatt ttcttcttaa tacatttttt
    10621 aagaaaaaac tctactttga atttttcaaa atttagaatt gattttaaga cacttagaaa
    10681 aattgtattt ataggttttc ctgattcaac tactgaatta agttgtggta tagttgtttt
    10741 actttttaac ttaagtctta ctaaatatat tggagaaaat gctcttattt attatagtgt
    10801 cataaattat ataaatacat tagtacttat gactatgatg ggtataactc aaggtatgca
    10861 accacttact agcttttatt atggtgcagg aaatatagat aatgtaaaaa aattattaaa
    10921 aatggggata aaagccacaa taatagcatc agttgctgta tttgcaattt gtatggcttt
    10981 ttcaggacca atagtctctt tatttataca tccagaagaa acaatgctat tcaatgaagg
    11041 agttcgtgta tttaaaatat tctctatatc tttcttattg gtaggaatta atgtaataat
    11101 ttctggattc tttgtatcag tagaaaaacc atcaatttct actgttatat ctcttggtag
    11161 agggcttgta atagttgttt taagtctaat ttcaatgata ctaatatttg gtggacaagg
    11221 aatatggatg actactattg tttctgagtt tatatgtctt attttatcat tagtttttct
    11281 aaaaaagaat ttttctacat tagactctaa tctaaataag gttgcttaga tgataagagt
    11341 gacaatatat gaaaatatct aaataaaaaa tacctattat acaatcttgc aaatattaaa
    11401 cctttgcaaa aattaggatt atataatagg tattttattt atgtaattat tttatttcta
    11461 aatatccata gccatctttt attaaagttc cttttacttc atattctaca ggttttttaa
    11521 atattggacc atctacgaca aatgtatgat actctccatg ttctccacat gggtctgcac
    11581 cagcattttt tatcttctcc acaagttctc tagttaattt ttttcctaaa aattcctctc
    11641 ctaagtgctt taagtttact tttgttacaa cactacaaaa tccactgtct ataaattcat
    11701 atactaaatc ctctctactc tcttgccata atggtaaatc tactttcata ccaactgctt
    11761 cacatctatc agtatcccat tttctatgac tttctatatc tatatctcca tatgcacata
    11821 tcgttgctcc catgttttta gcttttatta aagccctctc aaagctctct tcatatcctt
    11881 ctacgccaca ctcagcaact aataaaggta atcctatgtt gctacttact tgcttaagca
    11941 tattatattc taaattatgt gtccatgact ttccatgttc ttcacttata gttgtcaaga
    12001 gtgccacagg cttatatcct tttttcaaca ttcgatttaa tgctaatata ctatccttac
    12061 caccactaaa tgacattaca aatttttcat cagccaattt taacacctct ttcatctaac
    12121 tttgttttat taacataatt ataccaaatt tttgagttgc atagcaaatt atattaaatt
    12181 gttaattcac tataaagaat gtccctaata ttaaaagtat aacccctatg tatttgtata
    12241 aatgcattgt ttcttgtccc aaaattacgc tatcaataaa aaatccaact aaaacttggg
    12301 atacaagagt taatataagt gtatttgata ctcctaaatt tggaattgcc tttactgtaa
    12361 aatatattat tagacctcca aatactcctc ctaataacaa cttataatta aaaccattta
    12421 tagatactag acactttaag tcacctgtaa ctattatact tgccaaaaaa aatattgctc
    12481 ctacagttaa gcttataaag gtggccacta atgctgttgt actttttcct agctctccat
    12541 ttataaaagc ttcaagagct gtagaaccac ctgctaataa tgcaaatatt attgcccata
    12601 attttgtcat ggacaaacct ccttctatat aattctatta atagaagttt atgatacaac
    12661 ctattaatta attactaagt atagtttaat ttttatacac taggctttat ttggatatga
    12721 atttctgtta taattgttct atcgattttg gaggtgattt tatgtacaca aattttaata
    12781 tcgattttaa taactttaat aaaaaagaaa atgctattaa atttatgtta atgggagtat
    12841 tactcattat attaggatta ttatgcctta catttaagac attaggtata aaattaattt
    12901 cttggacttt tggtattgca cttttattct ttgcatattt aaatttaaaa aatattaatg
    12961 agttaaaaag atatgctact aaagaagaaa ttaaaccctc tattaatatc caatgggttc
    13021 taataattgc atgtatatta ctttttgtat ttcctcaaaa aatacaaagc atattttcat
    13081 tatttttagg attttattta atctttaatc aactagtagc acttgtaaat agtaagaata
    13141 atccttattc taaatttaca acttggaata tagttaagat tctatttggt atatgcctta
    13201 tattatctcc acttttttta tctagattta tagtatcgat aatgtctttt ttcataatat
    13261 tatttggact agttctattt ttctctggaa atacagcgag aaaatattaa ttattttctc
    13321 gctgtatttt tatattactt aaaatagaat ttgtttaagt tatctatttg tggtaatatg
    13381 ttttttgtac aagctcagcg tacaatttta ttagaaatag gtgatttttt ggataaattt
    13441 aaatatgctt ttgataataa aagatatcat acttggaatt actatcttag aaatactttt
    13501 ggagaaaaag tttttaaggt atctataaat gcaggattta cttgcccaaa cattgatgga
    13561 agtcttggtt atggcggatg tacctattgt agcaaagaag gctcaggtga ttttgctgga
    13621 aaccctaaag ataatcttat cagtcaattt tataatataa aagaaatgat gttaaaaaaa
    13681 tggcctcatg caaagtatat tggctacttt caagcctata caaatacgta tgctcctctt
    13741 gaagttttaa aagaaaaata tgagactata ttagaacttg aagatgtcat aggtctttca
    13801 atatccacaa gacctgactg tttgccagat gatgttgtag aatacttatc tgaactaaat
    13861 aaaagaacca atttatgggt tgaacttgga cttcaaacaa ttcatgattc tacatctaaa
    13921 attataaata gaggtcatga ttataaaaca tttgttgaag gtgttgaaaa actaaagtct
    13981 aaaaatataa aggttgtcgt acacattata aatggtcttc ctggtgaaga ttataatatg
    14041 atgatggaaa cagcaaaggc agttggtaaa atgggtgtag acggtataaa aatccatcta
    14101 ttgcatgtta taaaagatac tcctatggaa aagatgttac aaaatggtat gttaactctt
    14161 atggaacaag atgaatatat caatcttgtc tgtgaccaat tagaaatact tcctgaaact
    14221 atgataatcc atagactcac tggtgatggt aagagggatg aactagtagg tcctatatgg
    14281 agcctaaaaa aatgggaaat tttaaatcaa attgatgata ctttaaaggc tagaaattct
    14341 tatcagggct gtaagtttgt ttaatacaaa ataataacta tagaaatggt tatataaata
    14401 tagtcctttc tatagttatt tagtatttac aaaacaaaat attaatttgt acatttatac
    14461 aatatataat aaaaaataga tagtctaaac tgtatctact tatattttat ttgtactgtc
    14521 cactttattc tataaaattt ataaatagtt attttttaat accaaatata atttggtata
    14581 atttttgttg gactctaaaa tgtataccaa taaatccttt ttaagtatat aattctaaat
    14641 actttcagtt ttttcaatct gctttaactt gttggactta taaaatattc ttatttcatc
    14701 ataagaaaga ttaagtgcta ctactgcaga tattaacatc aacatcactc caaataactt
    14761 ccctaaggaa aaactttctc ctaatatagt gcaaccaatt attactgaac caactaattc
    14821 tactgatgcc acaacacctg caactgacaa ctctacacct ttagatatac ctgctgcata
    14881 aaaaatatat gcaagtgcag ctggaacaat gcctaacatt agcatacaag ataaaacatc
    14941 taaatcctta gcataattta agatttccca aggtcttgaa gaaggaatca taaatatagc
    15001 tccaaataag aaactgtata ctaatattgt tgaacttgaa aattccttta aagcattttt
    15061 actaatagtt ggcataagtg catatgctat tgctgataat accccaagta aaagtccaaa
    15121 agcgtttaaa ccttgaaaat ccaaacgtcc accagtcact gccataattg caccaataaa
    15181 acaaagaata agtgaaaata atttgcttct agtaatattt tctttataac atatttttga
    15241 aaatattgct aaaaacagtg gtgatgtata tagtaaaact gctgcaattg atacacccgc
    15301 aatgtcaatt gctttaaaat aacataaatt aaacattgcc tgacatataa tacctattat
    15361 tactgaatat ataattcctt ttttgcttat ctttaagatt tgtggtgtcc ttatagatga
    15421 atatacgcct aaaactataa atccaaaaaa caatcttgta aatgctactt gttctgatgt
    15481 cagtccattt cccattaaaa actttccaaa taatccaagt gtagcccata ataaccctgc
    15541 tattgctata aatacatacc ctttactttt ttctcccatt ttaaattccc ttctattttt
    15601 atttagtcaa tttacactct tctttttaag caaaaactat gccatcttaa atcaatatat
    15661 atcgactcta aaaccctagt ctttccaata atactaataa attttcaatt tttttatatt
    15721 tatttttcac ttactttaag ttgatttaga cttattttaa ttgtaaaatg agttatatat
    15781 aacttat
//
LOCUS       DAMJVI010000086        15484 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.090, whole genome shotgun sequence.
ACCESSION   DAMJVI010000086 DAMJVI010000000
VERSION     DAMJVI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15484
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.090"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(279..794)
                     /locus_tag="PC545_002772"
     CDS             complement(279..794)
                     /locus_tag="PC545_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HDF2457533.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDIAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
     gene            complement(1157..2227)
                     /gene="leuB"
                     /locus_tag="PC545_002773"
     CDS             complement(1157..2227)
                     /gene="leuB"
                     /locus_tag="PC545_002773"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDF2457534.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVETCRKNEAVLLGSVGGPKWDKCKGDKRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVEKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     EIYNENEIRRIGKRAFEIARNRNKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVKDPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLREDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEDAVQSVLNKGYRTID
                     IYNGVGNVVGTREMGELIVNEI"
     gene            complement(2234..2725)
                     /gene="leuD"
                     /locus_tag="PC545_002774"
     CDS             complement(2234..2725)
                     /gene="leuD"
                     /locus_tag="PC545_002774"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDF2457535.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIDEGDNIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            complement(2740..4017)
                     /gene="leuC"
                     /locus_tag="PC545_002775"
     CDS             complement(2740..4017)
                     /gene="leuC"
                     /locus_tag="PC545_002775"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDF2457536.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDVVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGEIGVDGALYKSMEFCGDGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTMEYINSHKCSTMTKDVNIYEADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILAAGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            complement(3974..5716)
                     /gene="leuA"
                     /locus_tag="PC545_002776"
     CDS             complement(3974..5716)
                     /gene="leuA"
                     /locus_tag="PC545_002776"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDF2457537.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESIRGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMATPNV
                     YADQIEWFCKNISCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIIGIYEDCTKLMVHDRHPYAGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKMESDRIGEELSSEAIFNLFKKEYIEVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARISYMGNEQRIVGIGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEIK
                     NGDDYDAENISQTC"
     misc_binding    complement(5840..6086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6234..6680)
                     /locus_tag="PC545_002777"
     CDS             complement(6234..6680)
                     /locus_tag="PC545_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457538.1"
                     /translation="MSKNKFIYMNYYDNGYFKKGMTIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTNESNYKIRLRALSLEDEKKLLNIIDEKMKKFKA"
     gene            complement(6701..7420)
                     /locus_tag="PC545_002778"
     CDS             complement(6701..7420)
                     /locus_tag="PC545_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HDF2457539.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASKKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYNGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGARVILGTDSHICYSIGEFENAEKVLEAVDF
                     PDELVINYHEDEIIEFFDINF"
     gene            complement(7538..8812)
                     /locus_tag="PC545_002779"
     CDS             complement(7538..8812)
                     /locus_tag="PC545_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDF2457540.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDVRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RIKAAKEDLFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            complement(9012..10379)
                     /locus_tag="PC545_002780"
     CDS             complement(9012..10379)
                     /locus_tag="PC545_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457541.1"
                     /translation="MENSNTNLGSESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIITACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWMFGASESTIGYSMDYLNIYLIGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIILPVLGLGISPFIMQSTESLVLIALNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCFTYSTILWL
                     LLMIFPAFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIKE"
     gene            complement(10629..11093)
                     /locus_tag="PC545_002781"
     CDS             complement(10629..11093)
                     /locus_tag="PC545_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HDF2457542.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHVLQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            complement(11098..11727)
                     /locus_tag="PC545_002782"
     CDS             complement(11098..11727)
                     /locus_tag="PC545_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HDF2457543.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRIIPLKKAKEAIFYPIVDFSEEDLKKFFDDCIMKKINESMKKVIYKNKEED
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNKFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKDGEVIEQINS"
     gene            complement(11761..12648)
                     /locus_tag="PC545_002783"
     CDS             complement(11761..12648)
                     /locus_tag="PC545_002783"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HDF2457544.1"
                     /translation="MKQTNTSIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLFLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSANILF
                     VVLALISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRDDAVEHRKILEDYDIENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNENNPTDIVLKDN
                     ALKINVILWIITCLIILIF"
     gene            complement(12677..13915)
                     /locus_tag="PC545_002784"
     CDS             complement(12677..13915)
                     /locus_tag="PC545_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457545.1"
                     /translation="MDGDILMSKILKYKNEMFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLITFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGASIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNMYNIFLSLLLVTIV
                     ISNVNGFIEILNIGIKSYPV"
     gene            complement(14299..15441)
                     /locus_tag="PC545_002785"
     CDS             complement(14299..15441)
                     /locus_tag="PC545_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2457546.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLSGGTIPKYNKELSF
                     LSKDGESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHDIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANSVKV"
BASE COUNT         5063 a         2832 c         1560 g         6029 t
ORIGIN      
        1 ctacttttta ttaaaataca gtatttgcaa catattcaca ctttattttt tcattttttt
       61 cttattacaa tggtttcgac aaatcagtaa ttaattttta taaatgaata aatcaaataa
      121 ttcctaaatt aattttttta gtatgtaaat ttgacgctat tacgataaat ctttttagta
      181 taactaattt atttttatct tgtattttta ttgtattttt gaattttatt ttttctaaat
      241 ataaaattta tgttttaaaa tgtaaaattt ttatttaatc aaagttcaat atattccact
      301 ttttctttcg attcttcaaa aaaccattct tcattagaaa aatcaccttc tgcaatttgt
      361 ccaataggca ttttcatata ctttccagtt gctgtcactg ctatatctcc atttggtaga
      421 attatttctc ctgtaccttc aaataatttt ctgttttctt tagttattct tccaacaact
      481 tttatagtag catctgttgg tactggtttc ttatatttca actctaaaga tactgttact
      541 ccccatattt ggtcatcatt tatacttaca gctcttccaa tagtctcatc caatattgca
      601 gcagacatac ctccatgaac tctcccagga taactttgat gccaatcctt tgtattacaa
      661 attgatacaa gctctccatt ttcaagttca taaaaatctg ctttaagtcc tagctcattt
      721 tgtgttccac aaactaaaca agatttacta ctattttgct tcttcaaaac tttatacttt
      781 gccaaaaaat ccatctccta accgacatta gtagtcgctt aatattaaat aattctaaaa
      841 tatattatat aatattaata aaatccctgc aaacttctaa taataaaaat aggctacttc
      901 aatatgataa gtttaattta aagtattatt ttccaaaaaa taactttatc aaaatagaaa
      961 ctcaaagtag atataaacct ctttttcgtt attaaaatag taaaaatata tttgacttaa
     1021 tataaaaaag agtgtctcat taactaataa agtttatttt gagacacccc tcataaaaca
     1081 aattatacta caatagattt ttactaattc tacaagttat atatttaaat tatattattc
     1141 aaattaaact ataattctaa atttcattta ctattaattc gcccatttcc cttgttccta
     1201 caacatttcc cacaccatta tagatatcaa tagttctata acctttattg agtacacttt
     1261 gtacagcatc ttcaatacac ttagcttctt cttctaagtt aaaactatgt cttaacatca
     1321 tgcttacaga tagaatagtc gcaattggat ttacaatatc ttttccagct atatcaggtg
     1381 cacttccatg tattggttca tacataccaa aactatcttc tctaagtgat gctgatggta
     1441 acatacctat agaacccgta atcatagatg cttcatcaga tattatatct ccaaatatat
     1501 tattagtcac tattacatca aattggctag ggtcttttac aacttgcata gcagcattat
     1561 caacatacat atgtgacagt tctacatctt caaactcttt agctaagtct tccattatac
     1621 ttctccaaag tcttgaactc tcaagcacat ttgccttatc tacacttata agttttttat
     1681 ttctatttct ggctatttca aaagctctct taccaattct tcttatttca ttttcgttat
     1741 atatttctac atcataagca ttttcgatac catcaatgat ttttcttcca cgttctccaa
     1801 aataaatgcc ccctgttaat tcccttacaa caacaaaatc aatgcctttc tcaactatat
     1861 ccgttctaag tggtgaagct tcttttatac tttcatacat tgtagctggt cttaaatttg
     1921 caaataaacc caatgactct cttaatttta aaagaccaga ctctggtctt ttatctccct
     1981 tacatttatc ccactttggt cctccaacag aaccaagcaa tacagcttcg ttttttcgac
     2041 aagtttcaac agttttatta ggtagaggtt ctccagtttc atcaatcgcg catcctccag
     2101 ctaatacata ttcacaatcg aatctatgat taaatttaca acaaatttta tttagaacct
     2161 ttataccttc atctataatt tccggtccaa caccatcacc ttttatgact gctatattac
     2221 aattcatact ccactacccc ctatttgctt ttattgagtt aattaagcct tcatttaata
     2281 taatattttg cataaactca ggaaatggtt ctgctttata ttcttttcct tttgttatat
     2341 tttttatcac acctgtagaa aaatctactt ctatattatc cccttcatct atgttattgg
     2401 cagcttcttc acattcaagt ataggaagcc ctatatttat agagtttcta aaaaatattc
     2461 tagcaaaagt agaagcaatc acacaggata ctccactttc ttttattaca attggtgcat
     2521 gttctcttga tgaaccacaa ccaaagtttt tagttgcaac tataatatct ccttttttta
     2581 ccttagaaat aaatttatca tcaatatcct ccatacaatg cgttgctagt tctttataat
     2641 cagctatatt taaatatcta gctggtatta ttacatctgt atctatattg tcaccgaatt
     2701 taaaaacact tccattggca atcataaatt ttacctccct taaattattt ctgatggttt
     2761 tgatattttt ccagttatag ctgatgctgc tgctacagct gggcttgcaa gatacacctc
     2821 tgaatcaaca tgccccattc ttcctacgaa attcctattt gttgttgaaa tggatttttc
     2881 tcctgctgca agtataccca tatgtccccc tagacatgga ccacaagttg gtgtagaaac
     2941 tgctgctcct gcctctataa aagtggtaat ataccctttt tctatagctt caagatatac
     3001 tttttgagtt ccaggaaata tcataactct aactccatct gctacttttt tgcctttcat
     3061 tatctcagct actacttcaa ggtctgatat tcttccattt gtacaagagc ctattacaac
     3121 ttggtctatc tttatatcct tatctatctc atctactgtt ctagtatttt caggaagatg
     3181 aggaaaggct actgtttctt ttagttcagc taaatctatt tcgtatactt catcatagac
     3241 tgcatcttca tctgcttcat atatatttac atcttttgtc atagttgaac acttatgtga
     3301 atttatatat tccatagtct tatcgtctac tggaaaaatt ccattttttg ctcctgcttc
     3361 tattgccata ttacaaatag taaatctatc atccatagat aagtattcta ctccatctcc
     3421 acaaaattcc attgacttgt aaagtgctcc atcaacacct atctccccta ttatatgaag
     3481 tattatatct ttaccactta tccatttatt tggtttattc ttcaaaacaa attttattgc
     3541 tccaggaacc ttaaaccaac attttcctgt agccatacct gctcccatat cagttgaacc
     3601 tacaccagta gaaaatgctc ctaatgctcc atatgtgcaa gtatgagagt cagcacctat
     3661 aattacatct ccagcagtta ctataccttt ttcaggaaga agaacatgct ctattcccat
     3721 atctcctaca tcaaaaaaat ttacaactcc ttttgatttt gcgaaacttc ttgtaaattt
     3781 acattgttct gcactcttta tatctttatt aggtgtaaag tggtctaaaa ccatcactac
     3841 tttagtctta tcataaacat cttcaattcc taatttttca aattctctaa tggctactgg
     3901 tgatgttata tcattaccta aaactacatc taaatctgct tcgattaatt gccctttctt
     3961 aacttcattt aatttagcat gtttggctaa tattttctgc gtcatagtca tccccatttt
     4021 ttatttcctc ctcaatataa cttttattta ttgcattcat aagtgcatta atagctgctg
     4081 tatttatgtt ttccgatata cttacaccaa aatagacttc attattgtgc tctattccta
     4141 cataagctac ccctcttgaa tgtgaccctt cctctaatgc atgttcccag taatatctaa
     4201 atttataatc tttcatgcca cattgcttta gtgcattatt aaatgaatct acaggtccat
     4261 ttcctatacc aacaatcctt tgttcatttc ccatataact tattcttgca gtcatctcaa
     4321 tcatattagt atcattagaa tcatcatttt catagtttaa ttcatccatt gattttattt
     4381 tatacttctt aactttataa gggctctcaa cttcaatata ttctttctta aacaaattaa
     4441 atattgcttc actactaagt tcttccccaa ttctatcaga ttccattttg actacatttc
     4501 caaaatcaga atgcatattt ttaggtatca taaatccata atctgtttcc attatataaa
     4561 cagctcctcc ttttccagat tggctattta tccttataat ttctttgtat tctcttccaa
     4621 tatcatgagg gtctattggt aaatatggaa cttcccagta atcattatct ctgtttttca
     4681 ttgctatcat gccttttcta atagcatctt gatgtgaacc tgaaaaagca caatgtacaa
     4741 gattacctgc atatggatgt ctatcatgta ccataagttt tgtacagtct tcatatatac
     4801 ctattatctt atctatattg ctaaaatcta gctctgggtc tattccttgt gtataaagat
     4861 taagtcccac atttactata tccatattcc cagttctttc accatttcca aatagtgttc
     4921 cttcaagtct atcggcacca gccaataatc caagttcact tgcagccgta caagttcctc
     4981 tatcattgtg agtatgcaaa cttagtataa tagaatccct acaacttata tttttacaaa
     5041 accattctat ttggtctgca tatacgtttg gtgttgccat ttcaactgtt gatggaagat
     5101 ttattattac ttttttttga ggagttggtt tccaaacatc tatcactgcc tcacatattt
     5161 caagtgcata atccatctct gttcctgtaa aactctctgg tgaatattca aaagtaaatt
     5221 ctgtttctgg atattttttt acttcttcat taaataattt tgctccttca actgctatat
     5281 ctattatttc ttgtttactc atattaaaaa ccacatctct ttgaagaaca gatgtagagt
     5341 tatataaatg tataattgct tttttacatc cccttattga ttcaaatgtt ttttctataa
     5401 gatgagccct agattgtgta agtacttgta tagttacatc atctggaatc atattctcat
     5461 ctattaattt tcttaaaaat gtatactctg tatttgatgc tgatggaaat cctacctcta
     5521 tttctttaaa tcctgtatca ataagcattt tgaacattct gactttttca tttacactca
     5581 taggtgttgg cagtgactga tttccgtctc ttaaatcgac actacaccaa attggagcct
     5641 taaatatttc attatctggc catttacgat ttactatttg cattttatca tattttttat
     5701 actttccaca cttcatgtta tttccccctt tttctgtttt ttgtattcag tatacctcat
     5761 atcgacgcaa tcctgcacaa ttcataagga taaatgtcaa ttttatcatt agaataaaaa
     5821 aaacctcatc tctaataaaa ttagagacga ggttttgctc gcggtaccac tctatttagc
     5881 tttctaaaaa gctcccttag taatagccaa tacaataatt ataatgacta tatttcctat
     5941 aacgtggaat tactccgatt attctaagat ttaaatctta gaaaacagct cctagacaag
     6001 ttcagaacta tcaactacta aatcacacca tcctttagct ctctgaaagt ttcattgttc
     6061 ttactacttc tattcatcac ttttttaatc atttaaattt tctaaatatt ttgatttaat
     6121 agttattata tacacattaa aatgtattgt caacacaaaa aataattatc tcaatttttt
     6181 atttttgata agcaattcta taaaacatgc taactattga aaacagtatg atattatgcc
     6241 ttaaactttt tcatcttttc atctatgata ttcaataatt ttttttcatc ttccaaactc
     6301 aatgccctta atctaatttt ataattagat tcgttagtgt atatatttaa tatatttttt
     6361 actacttcta ttttttctat tatcttataa ttaaactttt gactttgttt agattttata
     6421 ctgaatatac attgattatt tattaatatt gaatgattat cgagagtaat atatactttt
     6481 ccctcatatt tatttgaaaa atcacgacct tgtttaaata gaaaaactgc tgttattata
     6541 cataaaatat ataatattgt cgttgaatat ataaaaattg tagctaaaat tataagtgct
     6601 aaaaccatat atatagtcat tcctttttta aaataaccat tgtcataata attcatatag
     6661 ataaacttat ttttactcaa attttttcac tcccaagatt ctaaaaattt atatcaaaaa
     6721 attctataat ttcatcttca tgataattta tgactaattc atctggaaaa tctactgctt
     6781 ctaaaacttt ttctgcattt tcaaattctc ctatagaata acatatatga gagtcagtac
     6841 caagaattac tcttgcacca tatgttttac acaatgtcaa catatgactt acattttccc
     6901 aagtacccgt tctatgagag tttgaactta atgatgaatt atttatttct aaaaggatat
     6961 ttttatcttt agcttttttt actataggtt cataatctaa aggataccta ctgtcatctg
     7021 gatgtccaat tatttttacc ttaggtttat ccataacttt taaaatagca tttgtatttt
     7081 cttcttttgt accagaagct atacaaggtc tatgcaaact tgcgattata taatccaaat
     7141 gttgcaacat atcttcttga acatctaaat taccattata atccattata tttccttcta
     7201 ttcctctaag aatctttacg ccttgcactt cacgtggaag caaatgcaaa ttataaaaat
     7261 agaatggatg tggtcctcct ggcatattag gtccatggtc tgatattcct aagtatttta
     7321 tattcttctt actcgcctct tcgacatttt ccttgaaagt actatgtgca tgtccactta
     7381 ttagagtgtg acaatgtaca tccataatag actttttcat taaatttcct cctaatattt
     7441 tacataaatt cgtgaaaata gctaaagggc tatttcaaaa ctaaagctgt cataatagac
     7501 agtgtaacgt tttaaaacag ccccattttc aattatttta cacttctaaa tctttaaatt
     7561 tatctttaaa taattctcca atacttccta aagattcctc tacttctagg tattctgtta
     7621 tatcctcttc tggctctcta gttgcttctt ttattgaaac caacattctc ttagtctttg
     7681 gtttaaaatc aagaatctta actttaacct catctccaat attaacaaca ttagtaactt
     7741 tagccactct agtatcactc aattcagata ttggtaaata cgcatctaca ccttctttaa
     7801 tttgaacaaa agcccctttt tctattattc taagaacttt agcattgaca acatcatcaa
     7861 tttgtacttc ttttgctatt aaatcccaag ggtcattatt tatgtctttt aatgccaagg
     7921 caattttttt agtctcttta tttacatcca acacataaac ttctacttct tgtccctctg
     7981 aaacaacatc ttcaacactt tcaactcttg tccaagctaa attatttata tgtgctaatc
     8041 cttcaatccc accaatatcc acaaatgcac cataaggcat tatctttgtc accttaccat
     8101 ctcttttttg tccaacttca agagaattaa ataagtcttc tttagctgct ttgatacgag
     8161 ctttttcttc ttctctttct tgcttaattc ttgctctttc agcttctttt tctgccttaa
     8221 tttgagctct tctttcttct ctctctttat ttatacgctc ttgtaataca tctctatgag
     8281 atgctacaag tctatttttt ctaacatcca attcttttat ataaacttct aagttaagac
     8341 caacaaattt agatgtatct gtaataaatt ttgtatcaag ttgagaaatt ggtataaaaa
     8401 atgtatatgt attgtaatta gcaaatacac ctttatctat attcttttca acatttacag
     8461 ttattattct atgttcatca taagcctttt gtaactcggc aaaatcattt cttttgtcaa
     8521 gttgtaattt agataaagtt attgtaccat ctttttgaga tacttttgtt attacagcag
     8581 ttatttcatc acctatgtgg tatatatctt ctatatattg atttttttct atgttcaatt
     8641 cacttatagg tattattcca tcaaacccat agtctaatcc taattgtact tcatcatttc
     8701 tcactgcagt tatttttcca gttgtcaatt ctccaacttt cactttgctg aatacttgtt
     8761 cttgctggtc aagtaattct tgcattgtta aatcattttc cattataaac atcctttcct
     8821 cttattgatt atcatatctg taaatattat accattaata ttgcaaatac ttacattttt
     8881 cttactataa tttttaaaat aaattcaact tcatatattt atacattata gaaaatatct
     8941 tattaaatta aaatacaaat tctaattttt atctaataaa atattttcat aaaaaattta
     9001 tattgtatat cttattcttt tattgcttta ggttttgata gaaaacattt ataaaatata
     9061 aagaaagaaa ttgctgttat agtagcagct gttatatctg caactggctc tgctaaaatt
     9121 actgcaaaaa gtttttcatt aaagaatgtt ggaagtataa atattaaagg tattaaaagt
     9181 acaatctttc taagtagagc taaaaccaaa gatattgttg cttttccaag tgctaaaaat
     9241 gtctgttgac aagctatttg aatcccaaac ataaaagcac cagcaaaata tatttttata
     9301 ctccaactag tcatactaag taactgcgga ttcttattaa atatactaac aaaaaatgca
     9361 ggaaatatca ttaataaaag ccataaaata gtagaatatg taaaacatga caacaaacac
     9421 aacttaaatg tcttttttac tctatcaagt tgacctgaac cataattata acttattatt
     9481 ggttgtgctc cttgtgtaac ccccatatta ggaagcataa gaatttgcat tatactgctc
     9541 attatagcca ttgaacctac tgctaaatct cctccataca tttgtaattt actatttaag
     9601 gctataagta ctaaactttc tgtagattgc attataaatg gagagattcc caaaccaagc
     9661 actggtaaga tgatacttgc ttttggtatc atatacttct tttttatttt aagtatactt
     9721 ttctttccaa agagaaacat aagtacccaa atagcagaaa cgccttgtgc aattatagta
     9781 gctaatgcag ctccttttac acccatatta aagccaaata taaaaatcgg gtcaagtatt
     9841 atgtttatta aagcacctat agcaactgtc atcattccag ttgtagcaaa cccttgggta
     9901 tttataaaag tattcatacc cattgaaatc tgaacaaaca ctgtacctat taaataaata
     9961 tttaaatagt ccattgaata tccaattgta gattcacttg ctccaaacat ccaaagtaaa
    10021 ggctcttttc caatcttaaa acctatagtc aaaagtattg ctaaaatcac cagtacagaa
    10081 aaactgttac tcattatttt ctctgcctca tcattgtttt gctcacccat ttttatagca
    10141 gctaatggac ctcctcccat accgattaaa taactacatg cagttattat aattattatt
    10201 ggaaatgcca ctccaacacc agccatagca acttctccat tttccattct accaataaaa
    10261 atcctatcga ctatgttgta taaaacattt acaatttgtg cgataatagc aggtgtagca
    10321 agtttaaata ataatttacc cacactttca ctacctaagt tagtgtttga attttccatt
    10381 ttaaaacctc ctcaatccaa taattaaaat gtatcactaa aattttattc tatataaaaa
    10441 tttcatttta ttagcagaca aatttagagt actttcccta caagtaatac tatactatat
    10501 ttcttttgcc atagtaaaca ttttagaact aactgaagtt aaatacttta tctcattttt
    10561 tatttattat taaaaatttt tcacacatac tcataaaaaa actatccaag tatattactt
    10621 agatagtttt atattatatg taaagaaaga gctgaaacta taacccctga taacaaattg
    10681 ccaagaaata ctcccataaa agcatctttt attctcattt ttaatatgga agctatagca
    10741 gatgcagtcc acgttcctgt agttggcagt ggtataccta caaatagtat tataccaatt
    10801 atcgaaacac tttttaattt cttcattctt gagtttattt ttctatcaat tacatcagca
    10861 atccctttaa atagtttctt ccctcttaaa aattgtaata catgtctgaa tgtcaatatc
    10921 aagggaatcg ataccaatgt agaacctatt acactaaata cataaaccca tataggattt
    10981 aagcccattg caatacctat aggaatggct cccctcaatt ctgttacagg aaccatagat
    11041 aaaattaata tgtgtaaata tcccaaacta ttccctccta ttctaagtga cattaagtta
    11101 tgaatttatt tgctctataa cctcaccatc ctttttacta actaaaaatg catttttagt
    11161 caatgaaagc attggtaaat cactcttaga gtctgaatat gcatatgaat tttcataatc
    11221 aatctctatc ccaagtttac ctaatacagc ctttattctt tttacttttt caattccctt
    11281 acagttttct ccaatgaatt tatttttata tcttgaattt tcataaaaaa actccgtgcc
    11341 tataacctcg tctgcaagac cataatactt aaaatatttc atatatgcat atggagatgc
    11401 agttatcata attataacat tatcttcttc tttatttttg taaataacct ttttcataga
    11461 ttcgttaatt tttttcatta tgcaatcatc aaaaaacttt tttaaatctt cttcactaaa
    11521 atctactatt ggataaaaga ttgcctcttt tgccttcttc aatggtatta ttctaattaa
    11581 atacataaat agaataaaaa taatgtatgg taactttata atagttttaa aggttttatt
    11641 ttttaaagag taaataaaaa aatctatcat gctatcagta tttataacag tataatctac
    11701 atcaagtaat aatacacttt ttttcataag tatttcccca tcctatcact aaaaatatca
    11761 ctaaaaaatc aatattatta aacatgtaat tatccataag attacgttta tttttaaagc
    11821 attatctttt aagacaatat cagtaggatt attttcattt gtagtgttaa ttatataatt
    11881 atatctaagt acaccataaa ctacaaatat tgttgtaaat atcatatgtg gcttttctgg
    11941 attacttgta gaatacaatg cataacatac aattgtagaa cttaatataa ctatcatcat
    12001 ttgatttaga ttttctatgt cataatcttc caatattttt ctatgttcaa ctgcatcatc
    12061 tcttagagtt tctatttctt ttttacgttt tccaagagct aagtacagag ataagaaaaa
    12121 tgtacataaa attatccaat ttgataaact aacacttatg gctacacatc ctgcataaac
    12181 cctaagtata aaaccaagag atattgaaaa cacatctaat agtattatat tctttagttt
    12241 aagagaatat aatatattgt ttatcaaata gagactgaga attatagaaa gattaaaact
    12301 aacaactgtt gataaacaga ctgaaattaa agctaatact acaaatagta tattagcact
    12361 ttttttgctt attcttccac ttgccactgg tctattacat tttttaggat gttttttatc
    12421 tttatccatg tctatagcat cattcagtgc atatactgat gatgacatta gacaaaaaca
    12481 cacaaacgca ataaacgtat ttaaaaataa atttatattt agtatgttgt ttgaaaaaat
    12541 tatagctgaa aatacaaaga aattctttat atattgcttt attctcatta actttatata
    12601 atctgaaatt ttagtaagaa gccttatact tgtattggtc tgtttcataa acttactacc
    12661 ctttcaatat tattttttaa acaggatatg attttatccc tatgtttaaa atttctataa
    12721 atccattgac attactgatt actatagtaa ccaataacag cgatagaaat atattataca
    12781 tattcttttc attttttcta agcgctttcg taaagtaagc caataaaatt ataactgcaa
    12841 aactaaaatt tatagccata atacttgaat tgactagatt atatcctact ataccatgta
    12901 aagttagatt gaatataatt attcccatac atagcttagg aactcttttt tctgaatttt
    12961 caataactga taaaacacaa aaacataaag ctaataatgt aagaattact ggtattgtag
    13021 caacttctcc attttgcaaa aataaccctt ttttaatact tgctccagga aaataaaaaa
    13081 ccgaatacaa taatctctct ataatcattg caatcttttc caaaattggt tctccactta
    13141 taaatttcgt atcatactgt attacatttc caaggttcat aaaactctca taagactttg
    13201 ttgaaactac acaaatatat gccattagga taaatgtaat taaatgtttt aacatttttc
    13261 ctatttttaa tttaaactca aatatcatat taaaaaaata caaatatata ttactaatag
    13321 ttattccaaa tagaggaata gctgctaatt ctactaaata agaatttttt cctttcatct
    13381 tatttgcaat aactatgaaa aatattaatg aaaattggct atatataaat ctctctacat
    13441 ttaaagtcat aaataactgt ggaaaagaaa atatcattat catagtaagt aaaattaaag
    13501 ttaatctctg catttttatc tttttaatat gattaaagac taaagttatt gacatagcac
    13561 tagttattat ttgtagacaa attagtccaa acccataggc actattaaaa tctgttgtcc
    13621 caggatttgc tattgaagta caaaataggt atattggata tgcaaaaatt gatataattg
    13681 ctgaaaacaa tatgtgctta ctattgtctt gacttatagc aaatacattt aaattgaata
    13741 aaactcctgt atctgtatct aacaatacat cataagtatt gtttaaatca taaaatatag
    13801 tagtcttaga aaaaaatatc ttagttatta ttaagtaagt tattacaact aatataaaca
    13861 aaaacatctc gtttttatat tttaatattt tactcatcaa tatatcacca tccattcatt
    13921 atataggatt tgctgacacc tacaatttat tccttatatt taatagccat cttcataatg
    13981 ttcttacttg ttttattgta attgaataac tctttattta caaccacaat atttacttta
    14041 ttgatgttat aatcagtcaa gtattgttta tataacatag attagtgttg tataaaatga
    14101 gaatttgtaa gcaatattaa tgtaaaatat ttgaaaaact ttatataaaa atatacaatg
    14161 ttgcttatgg aattaaacta taacaaatta aagcataatt taaggagaca aaattataat
    14221 ctgtctcctt aaatattaaa actatatttt aaaacattac taaatactat acaaatttca
    14281 aatatagtat aaaattaatt agactttaac actattagct ccatcttctt cccatttttt
    14341 aatttctttt aatctatcta ttataggtaa atgctgagta cattcaactt cacattttcc
    14401 acaagatata cattctttag cttttgcctt actattcatt gtgtaattcc aatgtcctaa
    14461 gccataaact aactttgtca tttcttcatc actcttttta aacatctgct tttcattata
    14521 aaaaagcata taggctgggg tattaatacc ttttggacat actttacaat aaccacaacc
    14581 tgtacatatt tcgttcatat tcttatttaa tctagtctca atatcatgta tatcctcatc
    14641 tgaataaatt ctattttcgt ctgcaatttt acatgcatca tctatatctt gttttttatt
    14701 aaaccctatc aaagatatac ttatttgtga aattcctatg ttgaatctta aagctgattc
    14761 tatagtactt tcgccatctt ttgatagaaa agaaagttct ttattatact ttggaatagt
    14821 tccaccactt aatgggttca ttgccacaac accatatcca ttatcaacag catatttggc
    14881 accttttagc ctatatggaa agtttaatat atttattccc atagttacac cttcaaactt
    14941 attttcactt agaatatcta taacctcatc tcctggttga tgagatgaaa atactatatg
    15001 gtctatcaat ccttcttctt tacatttcaa taatccttca tattgacctc caggtctcat
    15061 tgctagttcg taatgttcca tctttctaat attccaaaca tgataaaaat ttattttaga
    15121 tacgcctaat cgttctattg atttttttac agcctctata gctttttctg atgtatcaca
    15181 tgcttttggc ttttctttag ttgatacata aaaatcggtc ttaccttcct ttatcatttg
    15241 tctaaaggct agtccaaata tatcttcact tctgtcatca caataccctg gtgctgtatc
    15301 aaagtaatta ataccttttt catacgcata tttgactaaa tcagcatttt cttgattact
    15361 tttttctaaa tcaaacctta atcctccata gccaactact gaaaccattt tccctgtctt
    15421 tccatattca acgtatctca ttgtaaattc ctccttagat atattaagac ttaatttctt
    15481 tata
//
LOCUS       DAMJVI010000087        15273 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.091, whole genome shotgun sequence.
ACCESSION   DAMJVI010000087 DAMJVI010000000
VERSION     DAMJVI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15273
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.091"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            240..1145
                     /locus_tag="PC545_002786"
     CDS             240..1145
                     /locus_tag="PC545_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457547.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVSK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(1305..2147)
                     /locus_tag="PC545_002787"
     CDS             complement(1305..2147)
                     /locus_tag="PC545_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDF2457548.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(2131..2889)
                     /locus_tag="PC545_002788"
     CDS             complement(2131..2889)
                     /locus_tag="PC545_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDF2457549.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHIADRYAEEGNIEGVERVQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(2906..3376)
                     /locus_tag="PC545_002789"
     CDS             complement(2906..3376)
                     /locus_tag="PC545_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2457550.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDDLLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDIKMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(3376..4119)
                     /locus_tag="PC545_002790"
     CDS             complement(3376..4119)
                     /locus_tag="PC545_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HDF2457551.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEITIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENDVEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLKLGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(4123..4536)
                     /locus_tag="PC545_002791"
     CDS             complement(4123..4536)
                     /locus_tag="PC545_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2457552.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVRLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(4562..5146)
                     /locus_tag="PC545_002792"
                     /pseudo
     CDS             complement(4562..5146)
                     /locus_tag="PC545_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5331..5714)
                     /locus_tag="PC545_002793"
     CDS             complement(5331..5714)
                     /locus_tag="PC545_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2457553.1"
                     /translation="MKISKLPEAELKVMRYIWEANDVLTSKELVAAMEEKYEWNESTT
                     FTVLKRLERREFLSTEKIGKLTHYNAMVKEKKYLRFETKEFLKNIHKNSISSLISALH
                     SKDDEVDEDKLIELEEHFKNLKEKE"
     gene            complement(5824..6201)
                     /locus_tag="PC545_002794"
     CDS             complement(5824..6201)
                     /locus_tag="PC545_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2457554.1"
                     /translation="MKEEILVEKLLRAELIVMEYLWKKDSLVTKKEIVKEMRQVYGWH
                     KSTIKILLKRLVDKGYLARDIIKFQSHYKIIIDNKEYYAFKKKVLKSSKSRKIMRSLT
                     TTHKSISKEKLDALEEYYRNLEE"
     gene            complement(6708..6851)
                     /locus_tag="PC545_002795"
     CDS             complement(6708..6851)
                     /locus_tag="PC545_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457555.1"
                     /translation="MSEFLLGVLASLTASFITYIISRKVKSHSGRSDFELDVKIKFNK
                     KRH"
     gene            7109..7297
                     /locus_tag="PC545_002796"
     CDS             7109..7297
                     /locus_tag="PC545_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457556.1"
                     /translation="MTKTILCDYCNKGINKDDNKYITFHKKSHMKTNICINCALNLID
                     KDKLNENIINNQHDYSKK"
     gene            complement(7707..8273)
                     /locus_tag="PC545_002797"
     CDS             complement(7707..8273)
                     /locus_tag="PC545_002797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457557.1"
                     /translation="MLGAKKEKEFNLLSFNEKKIEKHKCKNYHNDSSKDKYKYSQSEF
                     IAATLKEADDMNEEERRILEAEKRILETEKRVHQDNKEVKESIDKQFNDIKGIFKEYK
                     NDLDKDRIELKNDLARERAELKSDLKEAINEHKKVTEKDVSEIKNSMKGIEDRIDSTN
                     KWIIGLCITTIIGIATMAITIGISIWPK"
     gene            complement(8275..8766)
                     /locus_tag="PC545_002798"
     CDS             complement(8275..8766)
                     /locus_tag="PC545_002798"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457558.1"
                     /translation="MAYENGTDFIDYFPDCIEKYKEDNKIFEKAILLLNLYMQKPTWP
                     HCVNEYKSQAEKILGEDPEFIGKYGIQLRKFYDEDENAKVSKEFKNDLYNFSKSTYDI
                     LDNVYFSKSHPFNLSSIYSTSRDDDFIIKIFRYDNNFLELEMSKREVESLINYLSDLL
                     KEE"
     gene            complement(9016..9828)
                     /locus_tag="PC545_002799"
     CDS             complement(9016..9828)
                     /locus_tag="PC545_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2457559.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVIICPEKQFKTKSEEKSYKIPRVNAGGYDLLIELHLNASDGQGKGSEVLYYSN
                     KGLEYATRICKKLGTIFKNRGAKLDKGLYILNSSKPTAILIESFFCDNKDDYEKAKKF
                     GYEGMAKLIVEGVLNKNIGNDGVKLMYKHTIVYDGEVDKIPATVVGWGYNDGKILICD
                     IKDYIPGQTENLYIVGGGACNKIGSITKEKYTMIKGNDRFDTLYKALDFIDR"
     gene            complement(9828..10085)
                     /locus_tag="PC545_002800"
     CDS             complement(9828..10085)
                     /locus_tag="PC545_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDF2457560.1"
                     /translation="MDNLISFIPEQLLILVAALSIIGKGCKKYKQLDNKYIPVVLLIL
                     GIGFSIWMLGLSPVAVLQGVICWGISIGINQTYKQLKEENK"
     gene            complement(10105..10335)
                     /locus_tag="PC545_002801"
     CDS             complement(10105..10335)
                     /locus_tag="PC545_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HDF2457561.1"
                     /translation="MNEELFKENLKRHEVTINKHNDEIDELKVANIESKAELKALCEN
                     LNSLTSMLKWLIGTMITTLVGFFIFAVQKGIF"
     gene            complement(10393..11958)
                     /locus_tag="PC545_002802"
     CDS             complement(10393..11958)
                     /locus_tag="PC545_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457562.1"
                     /translation="MATVYEFNYTGSEQSVTLKPGKYKFECWGARGGALGTPFESGFY
                     YGYGGYCSGEITLKKETTLYLYVGIDGRKGYNFNSAGYGNGASGGGATDIRLIGGTWD
                     NEQGLLSRIIVAGGGGGTYDKQHGGDGGGLKGTLGTSSTGAAAHGGTQFEGGRGRDKD
                     GSCDGFFGKGATPKNPSSQSGGGGGWFGGAYPASGFGNGSGGGSGYVLTKDSYKPPGY
                     TPTSEYYFDNVVMTTGGNTTVVGNYSDGRAKITLLQALPFLNISSYNSTTATFKADHT
                     DPTLLTKIEIFIDDILKETITTDLILEKTINYTLEDNALHTLKIVVTDSTNATAEKIV
                     SLSKNIMPLGDDASLQDIASKVSEIKEGLINGKTSIINVLALKNIESSLNNSLVELSE
                     KVKESFDSSDASLQDLINQLTQANNTISQLNSKYKYSSGTFTYYTGAYPNGLNIGFRP
                     DIISYTVINAVEEKYYCVYTPLFYLFYNYTTGEKYLDVNHNHYGITFTDNGWIPKGPV
                     NNGVYSYIAVKCC"
     gene            complement(12086..12223)
                     /locus_tag="PC545_002803"
     CDS             complement(12086..12223)
                     /locus_tag="PC545_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDF2457563.1"
                     /translation="MNWYKIITDFYNNGNWTKEQVKTAVEKNKITATEYKEIVGEDYI
                     A"
     gene            complement(12224..12523)
                     /locus_tag="PC545_002804"
     CDS             complement(12224..12523)
                     /locus_tag="PC545_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457564.1"
                     /translation="MIYLGNFMETDEQNIKYIGMIHYKPTLLSEEELKNGILIEKLPV
                     QQYVENKEAKLFINIDTKEVFYRYTDIKSSIEDKVNSTEQTIADLTFQLMSNGVI"
     gene            complement(12520..13755)
                     /locus_tag="PC545_002805"
     CDS             complement(12520..13755)
                     /locus_tag="PC545_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457565.1"
                     /translation="MTFKELVNKVRNLVLEAKNVTIEDTENNFTSDNVEGALKEVFQN
                     GVNAKNNVVTALNSKGAEVTTSDTWEEIKNKIDIKEGRLDLRETILANSYSSYLVTNG
                     AIKYIEKYSGSLKALEYEEPYFYAIKGTHLIKINAIDESVIFDITLANANFSCICVTQ
                     EYLFISDNTKLYKINKLTGNEAQSIEGSYYKLCTYGEFIYGIYGDETSSILHKIRISD
                     MYIMLTKDMSSDRIYDFERGKFVCNNNGIYATTEHSNSSGLTECYLTKINFDFSVAKS
                     FRIGGYLYEKNIKFLNDFVFVSDSSRSIEVESGKKSGLAKYDANLNLITYGGSDRYEN
                     FEIYNGYIYTFNSLSSSPFIKINLNTLKEVNSYRTLINTNPQKGMFIINNIIFFIGGG
                     IHRNILSKKVYSDEKGEEL"
     gene            complement(13789..13971)
                     /locus_tag="PC545_002806"
     CDS             complement(13789..13971)
                     /locus_tag="PC545_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457566.1"
                     /translation="MNINNVVVRILAERILNGGLNPLKNREFQLDDVTNTEYRKAVED
                     YIIKNSGVVEGIEPTA"
     gene            complement(13971..14183)
                     /locus_tag="PC545_002807"
     CDS             complement(13971..14183)
                     /locus_tag="PC545_002807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457567.1"
                     /translation="MKKQVYYNSLDEKEKIISENSNLYVIEIYETLNENYLVLSSSPI
                     QDEKLSYEELENELLIMTNELQGGLL"
     gene            complement(14193..14831)
                     /locus_tag="PC545_002808"
     CDS             complement(14193..14831)
                     /locus_tag="PC545_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457568.1"
                     /translation="MTEKLTENASLGELMAALENVQTDFQTGKNNISSALGSPFIGTD
                     KFGTTKTKIETLKNVLVETINSKNVSATSSETFTNLIEKVNWIFQSIEIFSLKNRIQA
                     TTLNTPSIVYNEVSSIKGTLRFTGELKASKMRADYATAKIEILCGNRKEYFYVTDDTP
                     SASSSFVRFTKDIIVENGMDIKVQILLTSVGAGNLDGSYAARAEIEKLTILR"
     gene            complement(14845..>15273)
                     /locus_tag="PC545_002809"
     CDS             complement(14845..>15273)
                     /locus_tag="PC545_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDF2457569.1"
                     /translation="NGFKATVTHSLLTQRIVVNIIDATTKENAVTNFKIIDDNSIEVR
                     SETRSELNVYVINGNAETHFINATVDDNRVSEMTTYSSKKIEDSISSIQLIDTSISIT
                     DANDRFTSDKLDGVLEEIMVEISGQRTKGITIVNNLIDMI"
BASE COUNT         4915 a         2638 c         1647 g         6073 t
ORIGIN      
        1 aataagatta agaagctaat tgtaattttt ctgtaataaa aaacacttta aagttgaatt
       61 ttgaaaattc aatttacgat atttttattg taagtacctt acaacattat accaaattat
      121 tcctaaacaa tcaaaaactc catgaatatt taaaggtttt ctattttaaa aatcaccttt
      181 gactattaat ttatcaaata tgttataata gttttagata atttaaggag gattaaataa
      241 tggattttaa acaacttgaa gtatttgttg ctgttgcaaa acatcagagt ttttcaaagg
      301 ctgcaagaga acttttttta actcaaccta ctgtaagcgc acacatacag aatttagaga
      361 gagagctaga aactgttctt ataaatagaa gcaacaaagt tattacactc acaaaatctg
      421 gtgaaattct ttatgaacat gctatatata tattgaataa ttgcaaacgt gcaatttatg
      481 atataaaaga atattcagga aaaatagaag gtattataga tatagcatgt agttccatac
      541 ctgaaactta tattttacct gattttatga agagtttttc tatgagttat ccagatgtaa
      601 aattttccat aagccattat gattctcaat atgctatatc agaaatttta aatgaacgaa
      661 taagctttgg attagttggt tctaaaatca ataatcctca aatagagtac ctagacttat
      721 tagatgatga acttgttctt atcactcctt cagattttaa aattgataat aaaaataatt
      781 gtatcgatat aggagaattg gcttatctaa attttattat gagaaaagaa gggtcaggta
      841 caagaaactt aatactaaac acacttagta aaaataactt tccagtaagt aaattaaatg
      901 taatcgctca tgtagaatca aatgaggcta ttaaggaaat ggttagatta ggtcttggag
      961 tgtcctttat atcgtacatt tcagctatcg attatctcaa tgcaggtaaa ataaaatgtt
     1021 ataaaataaa ggatgttgat tttactagaa agtttttctt catttactct aaaaagaaaa
     1081 ctttttcccc attagaagat aaatttctaa acagattatg tgagtatttc gaaattataa
     1141 tataataact ttaataaagt agtgcttaat tttgagttta taactaatta ataagctaat
     1201 tataactatc acataaatgc ttataaatta ttatgaacca tctttctttt ttatttaatt
     1261 tagtcaaaaa atccctcgtt caaatgaact tgggattttt ttatttatac tactccaata
     1321 gcagccaata ctatacaaat aatcataact gaaattaata tcgtagttgt ctttaccttt
     1381 tttcctaata accaatacat aataccgaat atcatagctg gaagcataca tggcattatt
     1441 tggtctaagt aagtttgaat aggctctgcc cattctccac tacctacatt aacactaagt
     1501 tttaacatta cattagatgc agtcatacct ccaattacca taagccctaa cattgaagct
     1561 gcttccataa cttttgaaac tatacttgaa ccactcacat ctttaaagaa ttgagttcca
     1621 tacttaaatc ctttatctag acatatatag cgtataataa aatgaggaac atttattatt
     1681 aaaaggaata aaatcggtcc cattatgtta ccctgagaag ctaaagctat accaacccca
     1741 gctgctataa gctttaatgt tccccagaaa aatgaatctc ctatcccaga aactggaccc
     1801 attaaacttg atttaacagc atttatacta ttagcatcaa attctttatt ctttgcattt
     1861 tcttcttcca tagctccaga tatacctacc aaaagagtac ttatatgagg agtaactgac
     1921 ataaattcta aatgtctttt taaagcctta gatttctttt ctttatcatc tttatataat
     1981 ctacggatta ttggtgccat catgtatgca tatgctatat tttgcatacg ttcgtagttc
     2041 caagatgaat ctaatgtcaa tgaccttcta aaacatttcc aaaagtcttt cctcgttatt
     2101 ttactatctg gtgaaattgt ctctaacttc ttagaactcg ttgtcatcta ctattacctc
     2161 cttctctcct acaaagttaa catatattaa agctattgct aatccaaaca atgcaactcc
     2221 tgttactgtt atgtttaggt aagcaaccaa tataaatcct aataataaaa atgctactgt
     2281 tttcttatta agcatcattc tagctaacat ggcaaaacct attgctggaa tcacgcctgc
     2341 tgcaacatcc attccatgag ttacatatga tggtataaat gaaagtattt tttctattac
     2401 tggaacacct aatgaaaatc ctatacctac tattattgct aatattaatt tatttcctat
     2461 accaaataat aattgcacac gttctacgcc ttcaatattc ccctcttctg cataacggtc
     2521 agcaatatga gctccccatg taagtactac tgtcattaag aagttatcta taagcaatac
     2581 tagagttgct ataggaactg caagtacaac tgctgctgat acatcttgac ccgttaatat
     2641 tacaaacgct gtggcaataa tagaaccaga cacaaaatca ggaggattac ttgccccaac
     2701 aggaaatgca cccataaata ctaattctaa tgttgaacct attactatac ctgtatgaag
     2761 attacctagt gctaaaccaa caagtggtgc aacaactata ggtctattat gcattgtaga
     2821 acctacaaaa ctatgcatat atgcaaagaa tgcaattaga gttatcaaaa taacttgtgt
     2881 aaattccatt ttcatccccc taaaattata ttaatttcat tacatttgta gcatcatcat
     2941 caggaactaa ttgtacaaat acatttacgc cttctgaatc taactcttta atcatcttta
     3001 tatcatcttt tgatacatgc actgctttag atatattttc acgattatca tctgattttg
     3061 ttccaccaag gtttatagat ttaatagctt ttacctcttt agcaagtcta taaacatctt
     3121 ctacagattc acaaagtatc aataagttgt atttatcagt aacacctgag ttaagtgctt
     3181 ttatagaatc tgctatgctt ttcattacta acttaacatt agatggttta gccattttaa
     3241 gtcctgccat tctcaattca tcttttaata agtcatcact tgctatcaat atacagtctg
     3301 tgcttaaaaa ctttgtccat gtaaatgcta cttgtccatg tataagtctg tggtctactc
     3361 ttactaattt aatcattata attcctccct aatttttttg atttcatcca tgaatgtttt
     3421 tacgtgttct acactaacat ccccaaaatc tttgtaatta tctttaaagt aacttcctat
     3481 tattgccata tctcctaatt taagctgttt ttttgcattt tctaatgtaa ctccagcagc
     3541 tataaccaat gggaagtctc ctaaattttt acgaaacagt tctatttttt ccattgatgt
     3601 ttcttgacct gtagcatttt ctgtaactgc tatagcatca catcttgtca ttcctatttt
     3661 taaatcttct tcaacatcat tttctgataa tactggctga tatttaaaac gtacgcctcc
     3721 aattaaatac gctgggcatt ttgaacgttg taatttaaaa aactcttcta aagtagcttc
     3781 atctctaggt ttaacatgac ctactacaga atcaacctgt atataactag cattatattt
     3841 tgttgccagt tcaaatccca tagtatcaac atttaaacag tttactccat atggtattga
     3901 taaattagct tttgatacat attcaagtat tctttctaaa tcataataat taccatagta
     3961 attttctaac attattccat ctacaccatt ttcttcaaag attgtaatct cttttttagc
     4021 tctttcaaag atatcttctg gagtatctcc ttttaagtgt atcattgcta taattggttt
     4081 tttactttta aatactgata atattccctt ttttttcata aactaaaata cctcttcttc
     4141 ttctttaatt tgtaatgtca cgctttgtat tcctcttcta gattcttcca ctaattggtc
     4201 tatatcttca acggttaatt catcagaaaa ccttgtaaga agttctaata ccatattcaa
     4261 attcattcca gaaaataacc taacattatg tagattagtt aatcttaaca ttgctgtaca
     4321 tacacttgct ccatataagt cagataaaat tatatattca cattctttat cttctgatat
     4381 tgttttttct aattctaaag caaaatctga agcactttgt ccagggtaca aaccatacga
     4441 actgacacaa tctgctaaat ctcctactat catttttact gcatttaaaa gcccttttga
     4501 aaagtctcca tgagatgcta atattatctt tttcatactc atcctcctac taaattatag
     4561 attatatttc tgctgatact gcatatttgt acttatcact tcttataaat tgctcattgt
     4621 aatataagac atcattattt tctgtataca ccgattgtct tactttcaat agtggcatat
     4681 tacttgcaca tttaagatgg tcagaatatt ctttgtttga atgttttact tcaaatatat
     4741 tttctactcg tttaggtaat gtgcttatat tattagatat aacctccatt aaagatttat
     4801 cttctaattc ttcttttaat aaaaacatgt aatctattct aaaatagtca acttcaaaaa
     4861 taacaggtac ttcatctatt aatctaagtc ttttaataca tattaccttc tcttctagtt
     4921 caatacttaa agcttcagct acttgcttat ctgctttttt tattaaaaca gagattattt
     4981 tggtacttgg ttttgcatta atacgatgac aagaattagt aaaactattt cctgcacatg
     5041 ttgcttctat tctctctggt attgttacat aagtcccttt accacgtttt ttacataata
     5101 tatcttcttt tacaagttca ttaagtgcat ttctcacaga aattcttata ttttatttat
     5161 gtttatttat atttatttat gtttttatga gtacatttat ttacaaccta tctaaaacaa
     5221 tttctttatt ctattttatt tatatttctt tatttgtatt ttattttatt aaactgtcat
     5281 ggtggcaaaa aaggtggcaa aaataatttg ccacctaaaa taatctttat ttattctttc
     5341 tcttttaaat ttttaaaatg ttcttcgagt tcgattaatt tatcttcatc cacttcatca
     5401 tctttactat gtaacgctga tattaaactt gaaattgaat tcttgtgtat gttcttcaaa
     5461 aattcctttg tttcaaatct tagatatttt ttctctttta ccattgcatt atagtgtgta
     5521 agtttaccta ttttctctgt acttaaaaac tcccttcgct ctaacctttt taaaactgtg
     5581 aatgtagttg attcattcca ttcgtatttt tcttccattg cggcaactaa ttccttagaa
     5641 gttaatacat cgttagcttc ccaaatatat ctcataactt ttaattctgc ttctggtaat
     5701 ttactaattt tcatagtcac acccctttat cgaattatgt atatgttaaa ttctatctca
     5761 ataaagtttt ccaaacaaga gaatcacact aaatattacc atccaatttt ttccatctcg
     5821 aaattattct tctaaatttc tataatattc ttctaatgcg tctagttttt ctttacttat
     5881 agacttatgt gtagtagtaa gagaacgcat tatttttctg cttttgctag attttaacac
     5941 ttttttctta aaagcataat attctttgtt atctattatt attttataat gagattgaaa
     6001 ctttataata tctctggcca aataaccttt gtcaactagc ctttttagta gtatttttat
     6061 tgtactttta tgccatccat aaacctgtct catttctttt acaatttctt ttttagtcac
     6121 tagagaatct tttttccata aatactccat tactattaat tctgctctta ataatttttc
     6181 tactaatatt tcttctttca acttatgtca ctccttcgat tgttctagtt caagttaata
     6241 ataagagctt tttaagacaa aaactatagg taatttttac caaatattct actgttttag
     6301 tttgttttct tagaagaaaa ataactttca tatctataaa attattcaac tttcaaacta
     6361 tcctgttgaa taataactaa ttctattgta ttattttagt tgtagaataa aactaaatcg
     6421 gcaaaactag agaaatttag tggcataaat atatatgaac taagaattgt aaaatgagtt
     6481 ataagttata tcatccagtt atcaaaaaca tatactcttt ttgtttttgt gaaagttttt
     6541 taggtggcta ataatatata tacttagaga taaaataaaa tatatttcta atattcatag
     6601 ttttattttt tctcaaaatg cgatataata aagatgtaaa ttcgtatcaa aaacaaaaaa
     6661 agaaactaca atctattagc ctagagtgaa gtttcattaa ctaaatatta atgtcgtttt
     6721 ttattaaact tgatttttac atcaagctca aagtcactcc tgccagagtg gctttttact
     6781 tttctggaaa taatatatgt aataaagcta gctgttaaac tagctaacac tcctagtaaa
     6841 aattcgctca tttctccacc tccttccttt taagggatgg aatgtagaaa atgaagctcc
     6901 actcttagat tgtagttcca caagattatt cttgcatttt aattataaca taattttaca
     6961 attatcaaat atctattctc catttttttt atttatacaa tatattctat attgcaataa
     7021 aaaacattgt tttaaatata tatacatagg tatatactaa atatgttaaa cttaattatg
     7081 gaaaatataa aatgaaagga ataaaaatat gactaagact atattatgtg attactgtaa
     7141 taaaggaata aataaagatg ataataagta tattactttt cataagaaaa gtcatatgaa
     7201 aactaacatt tgtattaatt gtgcattaaa tttgatagat aaagataaat taaatgaaaa
     7261 tattataaat aatcaacatg attattcaaa gaaatgaaaa agcactctcc ataatgaaga
     7321 atgctctata taataatgtt tgacttagta aagatgtgtt ggggttacat atttactttt
     7381 ttattatatc atataacttt gtgtatgaaa aagaatttaa atcaatttta aggtgtgttg
     7441 agtaatgttc ttgattgttt atatgttgat gaatttcaaa aaaataagca ctcttataaa
     7501 aagagtactt ttggtatata ttcaagcatt tatctaatac aattatagca tgtcttatgt
     7561 tttagtatga taattttcgt tcgttttatt attacaggtt atactatagt tttatgtatc
     7621 ctgttaatat tttttactat tttttcaatg ttttgtttaa gtataaaaag agataactat
     7681 tttaattcta gttacctctt tttgtgttac tttggccata tagatatacc tattgtaata
     7741 gccattgtag caattcctat tatagtagtt atacatagtc caataatcca tttattagtt
     7801 gaatcaattc tatcttctat acctttcata ctattcttta tttcacttac atccttttct
     7861 gtgacctttt tatgttcgtt tatagcttct tttaaatcac tttttaactc agctctttct
     7921 ctagctaaat cattttttag ttctattcta tctttgtcta aatcattctt gtattcttta
     7981 aaaataccct ttatgtcatt aaattgctta tctatacttt cttttacttc cttgttatct
     8041 tgatgcactc ttttctcagt ctctaatatt cttttttctg cttctagtat tcttctttct
     8101 tcttcattca tatcatctgc ctcctttaaa gtagcagcta taaattcact ttgactatat
     8161 ttgtatttat ctttcgaaga atcattatga taatttttac atttatgttt ttctatcttt
     8221 ttttcattaa aactcaataa attaaactct ttttcttttt ttgctcctaa catactattc
     8281 ttcctttaat aaatcagata aatagtttat taatgactct acttctctct ttgacatctc
     8341 taattcaaga aaattattat catatctaaa tatttttatt ataaaatcat catctctaga
     8401 agttgaataa atagatgaaa gattaaaagg atgacttttt gaaaaataaa cattatctag
     8461 tatatcatat gtactcttag aaaaattata caaatcattt ttaaattcct tacttacttt
     8521 tgcattttca tcttcatcat aaaattttct taattgtata ccatactttc ctataaactc
     8581 tgggtcttca cctaaaattt tttccgcctg gctcttgtac tcattaacac aatgtggcca
     8641 tgttggtttt tgcatataca gattgagtaa taatattgcc ttctcaaaga ttttattatc
     8701 ttccttatat ttttctatac aatcaggaaa ataatctata aaatcagttc cattttcata
     8761 agccatttta aatcccccta aaataatatt atttaatatt attctacatt agaattattg
     8821 ttattgcact actatttttt atttcacctc cattatttta tcaatatata tatttgttaa
     8881 ctgtttatac acctagtttt ttggatttta aaatggctta tgattatctt taatatatca
     8941 ttttttattt tataaattgt cgtaatattg gcataaagta taagcaaaat gtaaaataca
     9001 ttaactatac tccttttatc tatcaataaa atctaatgct ttgtaaagtg tatcaaatct
     9061 atcattaccc tttatcattg tgtatttttc tttggttata gaacctatct tattacatgc
     9121 tcctccaccc acaatatata aattttctgt ctgacctggt atataatctt ttatatcaca
     9181 tatcagtatt tttccatcat tataacccca gccaactaca gttgcaggga ttttgtcaac
     9241 ttctccatca taaacgattg tatgtttgta catcagttta actccatcat ttcctatatt
     9301 cttattcaat acaccttcaa caattaactt agccatacct tcatatccaa atttcttagc
     9361 cttctcataa tcatctttat tgtcacaaaa gaaactttca attagtattg cagtaggttt
     9421 tgaactattt aaaatatata atcctttatc taatttagca cctctgtttt taaatattgt
     9481 acctagtttc ttacatattc tagttgcata ctctagacct ttattactgt aatatagaac
     9541 ctctgagcct tttccttgcc catcacttgc atttaaatgt agttctatga gtaagtcata
     9601 tcctccagca ttaactctag gtattttata agacttttcc tcactcttag ttttaaactg
     9661 cttttctggg catattatta catctacctt atgcccttct tttctaaatg tatctgctaa
     9721 tactggtgca agagatttgt tgtattgata ctcgttaact actccatcag cagaagtaca
     9781 tgctccactt tttaaaatac tgtgtcctac tgttatacat attttcatta tttattttcc
     9841 tccttcaact gtttgtaagt ttggtttata cctattgata taccccaaca aatcacgcct
     9901 tgtaagactg caacaggact tagtcctaac atccaaatag aaaatcctat accaagtatt
     9961 aataacacta ctggaatata tttgttatct agttgcttat acttcttaca gcccttacct
    10021 ataatagaga gagcagctac taaaattagc aactgctctg gtatgaaact tattaaatta
    10081 tccatttttt atcctcctaa ttaattaaaa tattcctttc tgaactgcaa atataaagaa
    10141 ccctactagt gttgtaatca ttgttccaat tagccatttg agcatacttg taagtgagtt
    10201 taaattctca cacaatgctt ttaactctgc tttagactct atatttgcca cttttaattc
    10261 gtctatttca tcattatgtt tatttattgt tacttcatgt cgtttcaaat tttctttgaa
    10321 aagttcttca ttcatgaaaa cctccttatt tatattaaaa taaggcttag aagttatcta
    10381 agcctttgat aattaacaac atttaactgc tatataacta tacacaccat tgttaactgg
    10441 acctttaggt atccatccat tgtcagtaaa agttattcca taatgattat gattaacatc
    10501 aagatatttt tctccagttg tataattata aaaaagatag aataaaggtg tgtatacaca
    10561 ataatatttt tcttctacag cattgataac agtgtatgaa ataatatctg gcctaaaacc
    10621 tatatttagc ccattaggat acgcaccagt ataataagta aaagttccac tagaatattt
    10681 atatttagag tttaactgtg atatagtatt attagcctgt gttaactggt ttattaaatc
    10741 ctgcaaacta gcatctgaac tatcaaagga ttcttttacc ttctctgaca attctactaa
    10801 agaattgttc aaacttgatt caatattctt taatgctaat acatttatga tagaagtttt
    10861 gccatttatt aatccttctt ttatttcact aactttactt gctatatctt gtagactggc
    10921 atcatcacct aatggcatta tattcttact tagacttact attttttctg ctgtagcatt
    10981 agtactgtct gtaacaacta ttttaagagt gtgtagtgca ttatcttcta atgtgtagtt
    11041 aattgttttt tcaagaatta aatctgttgt aatagtttct tttaatatat catctataaa
    11101 tatttctatt tttgttagta gtgtagggtc tgtgtggtct gctttgaatg ttgctgtagt
    11161 tgagttgtat gaggatatat tcaaaaatgg taatgcttgt aataatgtta ttttagcacg
    11221 accatctgag taattgccta caacagtagt atttccacca gttgtcatga ctacattatc
    11281 aaaataatat tcagaagttg gtgtatatcc aggtggctta taactatctt tagttagtac
    11341 ataaccactt ccaccgccac ttccattacc aaatccacta gcaggataag caccaccaaa
    11401 ccaaccaccg ccaccaccag attgtgatga agggttttta ggagtagcac ctttaccaaa
    11461 aaaaccatca caagaaccat ctttgtctct acctcgccca ccctcaaatt gtgtaccacc
    11521 atgagcagca gcaccagtag aagaagttcc aagagtccct ttcaagccac caccatcacc
    11581 accatgttgt ttatcataag ttccaccgcc accacctgca acaattatac gagatagtaa
    11641 accttgttca ttatcccaag taccaccaat aagcctaata tcggtagcac caccaccact
    11701 tgcaccatta ccatatccag cactattgaa attataacct tttctaccat ctattccaac
    11761 ataaagatat aaagtagttt ctttttttaa tgttatttca ccactacaat atccaccata
    11821 accataataa aaaccacttt cgaaaggagt tcctaatgca ccacccctag caccccaaca
    11881 ttcaaatttg tatttacctg gtttcaatgt aacactttgt tcacttcccg tataattaaa
    11941 ttcataaact gttgccactt aatccacctc tttcttaatt tatggtttta ttgtacctaa
    12001 ttttaaaact acaaacgtct acaaagtgta gatgaagtgt aggcaaaatg taaatgaatt
    12061 tatatatttt aagaaataaa aaagactatg ctatatagtc ctctcctaca atttctttat
    12121 attctgttgc cgttatctta ttcttttcta ctgccgtttt aacttgctct ttagtccaat
    12181 taccattatt atagaaatct gttattatct tgtaccaatt catttatatc accccatttg
    12241 acattaattg aaatgttaaa tctgctattg tttgttctgt agaatttact ttatcttcta
    12301 tgctactttt aatatctgta tatctataga aaacctcttt agtatctata tttataaata
    12361 actttgcttc tttattttct acatattgtt gtactggcaa tttctcaatt aaaattccat
    12421 tttttaactc ttcttctgat agtaaagttg gtttatagtg tatcatccca atatatttta
    12481 tattttgttc atctgtctcc ataaaatttc ctaaataaat cataattcct ctcctttttc
    12541 atctgaataa accttttttg ataatatatt tctgtgaatg ccaccaccaa taaaaaaaat
    12601 tatattgttt attataaaca tacctttttg aggatttgtg ttaatgagcg ttcggtagct
    12661 attaacttct ttaagagtat ttaaatttat ttttataaat gggctactag aaagtgaatt
    12721 aaatgtatat atatatccat tatatatttc aaaattttca tatctatctg acccaccata
    12781 agtaatcaaa tttagatttg catcatattt tgctaatcca ctttttttac cactctcaac
    12841 ttctatgctt ctgctactat cagatacaaa aacaaaatca tttaaaaact taatgttttt
    12901 ttcatataaa tatcctccaa ttctaaaact tttcgcaaca ctaaaatcaa aatttatttt
    12961 agttaggtaa cattctgtaa gaccacttga atttgagtgt tctgttgtag cataaatacc
    13021 attattatta catacaaatt tgcctctttc aaagtcataa attctatcag aggacatatc
    13081 tttagttagc attatataca tatccgatat tcttattttg tgaagtattg aagaagtctc
    13141 atctccatat attccataaa taaattcccc ataagtacat agcttataat aagaaccttc
    13201 tattgactgt gcttcatttc ctgttaactt atttatttta tataatttag tattgtcaga
    13261 tataaataaa tactcttgag taacacagat acatgagaag ttagcattgg ctaaagtaat
    13321 atcaaaaatt acagattcat caattgcatt aattttaatt aaatgtgtac ctttaattgc
    13381 ataaaaatag ggttcttcat attctaatgc ttttaaactc ccactatatt tttcaatata
    13441 ttttatagca ccatttgtaa ccaaatatga agaataacta tttgcaagta ttgtttctct
    13501 taaatctaat ctcccctctt ttatatcaat tttattcttt atttcttccc atgtatcgct
    13561 tgtagtaacc tctgctccct tggagtttaa tgctgttact acattatttt tagcattaac
    13621 tccattttga aaaacctctt ttaatgctcc ttctacatta tcacttgtaa agttattctc
    13681 tgtatcttct atagttacat tctttgcttc taatacaaga tttctaactt tattaactaa
    13741 ctctttaaaa gtcatttagt caccttcttt caataaaaaa agaacctact acgctgttgg
    13801 ttctattcct tctactactc cactattttt tataatataa tcctctactg cttttctgta
    13861 ttctgtgtta gttacatcat caagttgaaa ctctctattt ttcaaagggt ttaaccctcc
    13921 atttaatatt ctttctgcta atattcttac cacaacatta tttatattca ttataacaat
    13981 cctccttgta attcattagt cataattaat aattcatttt ctaattcttc ataactaagt
    14041 ttttcatctt gtattggact acttgataac actaaataat tttcatttaa agtttcatat
    14101 atttctataa catataaatt agagttttca ctaattattt tttctttttc atctaaagaa
    14161 ttataatata cttgtttttt cataatttat tcctacctta atattgttaa tttttctatt
    14221 tctgcacgag ctgcatatga accatctaaa tttcctgctc ctacagatgt taataatatt
    14281 tgtactttta tatccatgcc attctcaaca ataatatctt ttgtaaatct tacaaaagaa
    14341 gaagatgcac taggagtatc gtcagtaaca taaaagtatt cttttcggtt tccacatagt
    14401 atctctattt ttgctgttgc ataatcagca cgcattttag acgctttaag ttcgcctgtg
    14461 aatcttaatg tgccttttat actagatact tcattgtaaa cgatgctagg agtgtttaaa
    14521 gttgtagctt gaattctatt ttttaaagaa aaaatttcta ttgattgaaa aatccagtta
    14581 actttttcaa tcaaattagt aaatgtttca gatgatgttg ctgaaacatt tttagaatta
    14641 atcgtctcca ctaatacatt ttttaatgtt tctatttttg ttttagttgt accaaattta
    14701 tctgttccaa taaaaggact acccaatgca ctagatatat tatttttacc agtttgaaaa
    14761 tcagtttgta cattttctaa tgctgccata agttccccta aactagcatt ttcagttaat
    14821 ttttctgtca tattttcacc tcgcttatat catatctatt aaattattca ctatagttat
    14881 tcctttagtt ctttgaccac ttatttctac cattatttcc tctaatactc catctaactt
    14941 atcacttgta aatctatcat tagcatctgt aatacttata ctggtatcta taagctgtat
    15001 actgctaata gaatcctcta ttttcttaga tgaataagta gtcatttcag acactctgtt
    15061 atcatctaca gttgcattaa taaaatgagt ttctgcattt ccatttatca cataaacgtt
    15121 taattctgac cttgtttcac ttctaacttc tatagaatta tcatctataa ttttaaagtt
    15181 tgtaactgca ttttcttttg tagtagcatc tataatattt acaactattc tctgtgttaa
    15241 taaactatgt gttacagttg ctttgaatcc att
//
LOCUS       DAMJVI010000088        14798 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.093, whole genome shotgun sequence.
ACCESSION   DAMJVI010000088 DAMJVI010000000
VERSION     DAMJVI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.093"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            122..634
                     /locus_tag="PC545_002810"
     CDS             122..634
                     /locus_tag="PC545_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDF2457570.1"
                     /translation="MSDFKEILAEEIKNNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QIGFNIEHLNDTPYFKEAKMAIICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKED"
     gene            complement(789..1850)
                     /locus_tag="PC545_002811"
     CDS             complement(789..1850)
                     /locus_tag="PC545_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDF2457571.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGRNIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1974..2867)
                     /locus_tag="PC545_002812"
     CDS             complement(1974..2867)
                     /locus_tag="PC545_002812"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDF2457572.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIESGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPAGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGNMIVSVTDNENIHYDIIKIPEELK
                     FCAMIPNFKLSTEKARGVLPKEIPYSDGVFNVSRVALLISALLNKNFDLLKVACQDKL
                     HQDYRGTLIENYNDIVEKSEQLNSIGVFLSGAGPTIMSLIKENDDSFVDNMKNYLQKL
                     KSDWEIKELCCDSNGAVLNII"
     gene            complement(2877..4358)
                     /locus_tag="PC545_002813"
     CDS             complement(2877..4358)
                     /locus_tag="PC545_002813"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDF2457573.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSEFPNVKNELIN
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVSNSDINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLSDKGVYKISDDMEKNLASFYGEYSNEEEVKSTINNVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(4466..4697)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5499..6407
                     /gene="trxB"
                     /locus_tag="PC545_002814"
     CDS             5499..6407
                     /gene="trxB"
                     /locus_tag="PC545_002814"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2457574.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKVKVVKTSSGEHKAKA
                     VILANGVERRKLGCAGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARENIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(6497..8320)
                     /gene="typA"
                     /locus_tag="PC545_002815"
     CDS             complement(6497..8320)
                     /gene="typA"
                     /locus_tag="PC545_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDF2457575.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEDLSPLFKTIVNHVEAYPNYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            8532..8774
                     /locus_tag="PC545_002816"
     CDS             8532..8774
                     /locus_tag="PC545_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457576.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            8922..9113
                     /locus_tag="PC545_002817"
     CDS             8922..9113
                     /locus_tag="PC545_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HDF2457577.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
     gene            9404..11905
                     /locus_tag="PC545_002818"
     CDS             9404..11905
                     /locus_tag="PC545_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDF2457578.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQNVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYDPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMTNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKSLFSLSPNMDSLVAIGTLAAFLYSLYTTLQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAIVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINDESILLGNRKLMNDNNIKLGDLEEKSNILAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNAKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            12065..12733
                     /locus_tag="PC545_002819"
     CDS             12065..12733
                     /locus_tag="PC545_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457579.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            12717..13994
                     /locus_tag="PC545_002820"
     CDS             12717..13994
                     /locus_tag="PC545_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDF2457580.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEIKADQERITKAINNIINNAIKYSPEESELI
                     IRLYDKNKRISKKTSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVVFTVEFKN"
     gene            14395..14616
                     /locus_tag="PC545_002821"
     CDS             14395..14616
                     /locus_tag="PC545_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457581.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
BASE COUNT         5656 a         1877 c         2269 g         4996 t
ORIGIN      
        1 tttacttatt tgcaataata tatttaaaaa agtattttat tttcaaaact agacttcatt
       61 tataatttat catgaaaata aaaggaatat aaatataaga gaatgagagg ttattagaga
      121 tatgagtgat tttaaagaaa ttttggcaga agagattaaa aataatccat ttggattgat
      181 aggtaaagat tggacactta taacagcaga aaatgaaggt aaagtgaata caatgactgc
      241 tagctgggga gggcttggag ttatgtgggg aaaagatgtt gcttttgtag taataagacc
      301 acagcgttat actaaggaat ttatagataa tacagataaa ttttctctta cattttttga
      361 tgaagatttt cgtaaagagc tttcatattg tggaaaagta tctggtagag aagaggataa
      421 gatatcacag attggattta atatagaaca cttaaatgat acaccttatt ttaaggaagc
      481 taaaatggct attatatgca aaaaaatgta tagtcagaaa cttgaacccc aatgttttat
      541 tgctgaaggt atagatggaa gatggtatcc acaaaaagat tatcatactt tatatatagc
      601 tgaaataacc aatgtacttg taaaagaaga ttagttataa gggttaatac tatctctaat
      661 taaattaaaa tttcaataaa aggaaattga cacaaaaaag ctaaggacat aaatatgttt
      721 ataaagatta ataaataacg tatttaaact ccttagcttg ataaaatttt aatttagttt
      781 tctttaaatt ataattcaga aatcaattca ttgataacat cttcaacagt acttaattta
      841 tcaattttat aggcatcatt tccacaaaat aaaagtgaat tttcaacatc tcctttaact
      901 gcattgatta gagcttgaga aatacagtaa ggagtttctt ttggattaca aggaatcaaa
      961 caattatagc acttctttat ttctggacga tttattttaa cttcttctat aaatttgttt
     1021 cttatggcac gtccaggaag acctacagga cttttaacta tatgaacatc ttcttcacta
     1081 gcatttatgt atgccatttt aaagttctca tgggcatcac attcgtaagt agcaacaaat
     1141 cttgtaccaa cttgtacacc acttgcaccc atatctatat attttttaac atcactacta
     1201 gaagttatac caccagcagc aactactggt atgtttctac caaatttttc tccataagta
     1261 tttgcaacct ttaaaatttc aacaaattcc ttatcatagt ctatagaatc tatgttatca
     1321 agttcctcgt tagagtatcc aaggtgacca ccagcttttg gaccttcaac aactattaag
     1381 tcagcagtag ttttctcttt tctatcccac atttttaaaa ttacattagc agcttttgca
     1441 gttgaaacta tgggtgctat ttttacatta cttcccttta ctaatgatgg tagcttatta
     1501 ggtaatccag ctccagatat tataaggtct acaccagctt taacagcttc ctttacgtat
     1561 gtttcatatt ctttcatagc taccataaaa ttaataccaa taataccacc atttgagatt
     1621 tctttggcct tagaaatatg tttttttata gctcttaaat ttgcatttat agtattagtt
     1681 tcaaaatctt cctcatcata tccaatttga actccagaaa ttacaccaac accaccaagc
     1741 ttagcaactg caccagctag tgatgaccta gatataccaa cgcccattcc accttgaatt
     1801 ataggaactt ttgcaactaa atctctaatt attaactctt ttatgataat ccctcctcaa
     1861 tgtaaacatg tttaaagaac tagattataa tacttaatat tattacctag tcctaattat
     1921 tatactacaa attataataa aagagtagtt atattttgta tagaagacga taattatatt
     1981 atatttaaaa cagctccatt agaatcgcaa cataactctt ttatttccca atcagatttt
     2041 agtttttgta ggtaattttt catattatcc acaaaactat catcattttc ttttattaaa
     2101 gacattatag taggtccagc tccacttaaa aatactccaa tactatttaa ttgttcagat
     2161 ttttcaacaa tatcattgta gttctcaatt agagttcctc tataatcttg atgcaatttg
     2221 tcttgacaag ctactttaag taggtcaaag tttttgttta gtaaggcaga tattagtaaa
     2281 gcaactctac taacattgaa taccccatca gaatatggta tttcctttgg taaaacacct
     2341 ctagcttttt ctgttgataa cttaaaatta ggtatcattg cacaaaattt aagttcttca
     2401 ggaattttta taatatcata atgtatattc tcattatcag taacagatac aatcatattt
     2461 cctaaaatag caggagcaac attatctggg tgaccttcta tttctgaagc tatatttagt
     2521 aattgatttt tatctaaatt agcacctgat agagcatttg cagctataac tccagctact
     2581 atacaagagg cactactgcc aagacctcta catataggta cttcactttg gatttttatt
     2641 ttgataccag caggtatttt ttctggttta actctatcaa aaaagtactt catagatgta
     2701 taaactaagt tgttttcgtt tttgtaagca tcttcacaac cttctatttc aagaccactt
     2761 tcaatttctt caacataaaa tttattgtat atatttaacg caattccaag acaatcaaat
     2821 cctggaccta tattagcact tgttgcagga actataatct ctaacataat aatcacctat
     2881 acctttaaga atttttttat agctagtttt aattcatctt tttcataaat atctttatgt
     2941 agtatttctg catttttaag atttttaata ggttttggaa tatctatttt agaaatactt
     3001 gataatctat caattatagc aaagtcatca aggtctttaa aatcacaatc tatagacttt
     3061 agaacatctt cagaaaactt gaatggactt gcagtactta caataacagt ttttgtgtta
     3121 tcagaagttt cttttttata tttttcataa caattgtatg caacagcagt atgagtatct
     3181 atcaaataat tataattttt aaacacattg tttatcgtgc ttttaacttc ttcttcattt
     3241 gaatattctc catagaatga ggctaaattt ttttccatat catcacttat tttgtagact
     3301 cctttatcac taaggtctga taaaagctta tttacaatat cagtatttct atttgatatt
     3361 tcaaacagta atcgttctaa attacttgaa attaatatat ccatagaagg agaagtagtt
     3421 aatttcaatg ttctattttt atcatataca ccagttttaa agaaatcata aagaacatta
     3481 ttatcatttg atgcacatat taatttgttt attggaagac ccatttgttt ggcataatat
     3541 ccagctagaa tattaccaaa attaccagtt gggacaacaa aatttatttt atcatttaaa
     3601 tttatatcac tattgctaac taatttcata tatgaataaa aatagtatgc aacttgtgga
     3661 agaagtcttc ctatattaat tgaatttgca gaagataata tataattatt atccaatagt
     3721 ttttgattaa attctttatc agaaaaaatc ttctttacac ctgattgtgc atcatcaaaa
     3781 tttcctttta taccaactac atatgtattt tttccagttt gagttttcat ttgaagtttt
     3841 tgtactgggc ttacaccatc ttctgggaag aatacaatta tctttatttt atctatatct
     3901 ttaaatccct caagtgcagc tttacctgta tctccagatg tagcagtaag aatcacaaca
     3961 tctttctcta aattatcttt ctttatagat gttttaagaa gatgaggcat tatagtaagt
     4021 gccatatctt taaatgctaa agttggaccg tgatatagtt ctaagaaata agtatcattt
     4081 actttattta atggagctat actactacaa tcaaactttt catcatatgc attttctata
     4141 caatttttaa tttcatcttc agtaaaatca cataaaaatt tactaagtac aaaaaatgct
     4201 atctgagaat acgttaaatt tactaaattt ataagttcat tttttacatt gggaaattca
     4261 cttggaacat aaagaccacc atcatttgat attcctttta ttattgcttg tgatgcagtt
     4321 actttttctt cggtgcctct cgtacttgaa taatacattt tttataatcc tccatatatt
     4381 ttaaattttt aattagctaa ttaaattaga tacagaatat tttaaaaact gaatttaggt
     4441 aataaaaaaa gaccctcatc cctaaaaaag ggacgagaat ctgtttcccg cggttccacc
     4501 caaattgatt ttaaaaaatc cacttgatac tttaacgcaa gtaataaaaa cgtctattcc
     4561 tactaaaatc attaagataa cttcggatag aagctcctag gtagttttca atccttctaa
     4621 ttagggaaac atttcagcta gtagtttcct tctctggtaa tcgattcaga tttactcttc
     4681 ctaatcaatg cctttcaata ttcttaatat tattaattat tctagtttat aaaacagaca
     4741 aagtcaagta ttaatataaa aatttggaaa aaatattttt taaattgtaa aaaaattgta
     4801 atcaaattga gtattagaaa aatatttttt taactttttt aaaagtttaa aaacctagat
     4861 tttcatagtg atgaagctgt ttttaatgaa agtaatcaaa agataactat taaatatctg
     4921 taatattaca attttatact acactatatt acaaaaaaag tttacaaaac tttaatttga
     4981 aaaatgtaat tatatggtat atcatataaa tataagtaaa tagtgctaat atttaaacta
     5041 ttaatcataa ttggttcaac aaaagtatat agttaataat gtttaatttg aagaaattgt
     5101 tagtgtgaaa ttacatatat attcaaatta aattcataaa tatttcacat aataatagta
     5161 tactgtaaac aaaaggaggt attaaatatg ggaatagtac ttataacagc agcattagtt
     5221 ttggttatgt atggactttc aattctatca tatatatcag aaagcaatat attagaagac
     5281 actgtaaatg ataaccaata aggttttgaa ttatataaat aagtgagaac tgtaaataaa
     5341 agttaatttt tatttgcagt ttttttatat atatgtaaaa taaagttaat taataaaaaa
     5401 taatgttttg tttatagtat atatggataa taatagtaaa gtataagaat gatgagaata
     5461 atatataaga aataataata taaagagagg taaacgttat ggtagatatc attgtaattg
     5521 gtgctggacc agcaggttta acttcagcaa tttatgctat gagagcaggg ttaagcgtta
     5581 cagtatttga aaaaaatatt tatggaggtc aagttgcaag tacctctgag gtagaaaatt
     5641 atccagcagt tcaaaagatt tctggggtag aattttctaa taatatatac aatcaagcag
     5701 ttgctcaagg tgttgatatt cagtttgatg aggttgaaga gataaattta gagggaaaag
     5761 taaaggttgt aaaaacttct tcaggtgaac ataaagcaaa ggctgttatt cttgcaaatg
     5821 gagttgaaag acgtaaatta ggttgtgcag gggaacaaga atttacaggt agaggagtat
     5881 cttactgtgc aacatgtgat ggagcctttt ttaaagataa agaagttgct atagtaggtg
     5941 gaggaaatac tgcactagaa gatgctttat ttttagctaa taattgtaca aaagtgtatt
     6001 taattcacag aagagatagt tttagaggtg aagaagtgtt ggagaaatct gtgaaagcaa
     6061 gagagaatat tgagatactt tacagtcatg gtgtagaaaa aatagaagga gaaaagactg
     6121 tatcgaaaat agaagtaaaa aacttaaaga ctgaagaaaa aagaactata gatgttagtg
     6181 gtatttttat tgctataggt cttaaaccta ataataaaat gtttgaaaat gttcttgatt
     6241 tggatgaagg tggatatata atttcagatg agtcttgtac aacatcagta gaaggtgtat
     6301 atgtagcagg agatagtaga actaagtttt tacgtcaaat aataacagca gcatcagatg
     6361 gagctattgc agccgttcaa gcagctaatt atataaatgt tgaataatat aaatttcaaa
     6421 taaaaatagc catataaact gaaataattt ttcagtacta tatggctatt ttgttaatta
     6481 gaggtaaatg tacgttttat ttaccttgtc tagatttgtt atatcttaat ctatcatgtt
     6541 catttaagaa tctttttcta agtcttatag aatcaggtgt aatttcaact aattcatcat
     6601 cttctataaa ctctagagct tcttcaagag taaatattct aggaggagat aattttatag
     6661 catcatctga accagaagca cgtacgttac tcattttctt gtttttacaa ggattgacta
     6721 ccatatcatc ttttctggaa ttcataccaa ttatcatacc ttcgtaaact tctacaccag
     6781 ggtctacaaa cattacagct ctatcactta aggcattaag agaatacccc atagtaacac
     6841 caggtccttg tgctattaaa acaccatttc ctcttgaagg tatttctcct ttaaattctt
     6901 caaatttttc aaatgaacga actaaagtac cttctccacg agtagcgttt ataaattcac
     6961 ttcgatatcc taaaagacca cgagtaggtg ctaagaactc tatttttaca tagtctcctt
     7021 ctattgacat agactccatc ataccttttc tcatgtttaa ttcatttata atagtaccag
     7081 agtaaacttc aggacagttt acaacaactc tttctattgg ttccattagt tttccatctt
     7141 ctttatgcat taaaacttca ggtttagaaa caccaacttc ataaccttca cgtctcatat
     7201 tttcaagtag tatagataag tgaagctctc cacgtccaga aactttgtat ccatcagtag
     7261 tatcaagagg ttcaacttta agaccaacat ttacttcaag ttctttttca agtctatctt
     7321 tcaaatgtct agttgtaacg aattttccac tttttccaac gaatggagag tcgtttacta
     7381 agaagttcat agaaagagta ggctcttcta tttttatcat ttccataggt agtggactat
     7441 ctaaatcgca tatagtttca cctatagata tatcaggaat accagctata acaactattt
     7501 ctccactagt agcttcatca acttcaactt gttttaaacc ttcatacaca gaaagttttg
     7561 atacttttcc tttagataca acagaatctt ctctacatat agcaacttgt gtattgtttt
     7621 taagagtacc tttgtaaatt ctacctatac caagtctacc tacataatca tcatatgcaa
     7681 gagctgatat ttggaactgt agaggttcat tatcgtaatt aggataagct tcaacgtgat
     7741 ttacaatagt tttaaataga ggagaaaggt cttcactatc atcatccatt tcaagtttag
     7801 caataccttg tttagcaata ccatatatta taggaaattc gcattgctca tctgttgcat
     7861 ttaagtctac aaataaatcg aaaacttcat taactacttc ttccgctctc tggtcttttt
     7921 tatcaatctt gtttataaat aatattggtt ttaaaccgaa ctctaaagat ttttgtaaaa
     7981 cgaatctagt ttgaggcatt gggccttcac tagcatcaac taatagaata actgtgtcaa
     8041 cagttttcat aacacgttca acttcagaag aaaagtcact gtgtcctgga gtatcaacta
     8101 tgtttatttt gtaatcttca tagtttattg cacagttttt tgaatatata gttatgcctc
     8161 tttctttttc aaggtcgtta ctgtccataa cgcagtcttt aactacttcg ttctttctaa
     8221 aaacaccgct ttgagaaagg aaagcatcaa ccaatgttga ttttccagcg tcaacgtggg
     8281 ctataactgc gatatttata attttatgtt tttgtgacat ttttgttgct cccttcgatt
     8341 aaaataactt attaattata gcacaatata ttattaggta caaggaggaa tatattaaat
     8401 tgtattaata tagtttaaat tggataaaac tgttagtttt gcatctgaaa attattctgt
     8461 ttaaaaaaat tttttagaca aataataacc ttaatagata aaaagattaa taaaagagta
     8521 aaggatgata tatgttggaa gtaaccaaaa taataattaa atattttgca agtgaaagtg
     8581 atacttttgt aaaagaagaa gtttgtaatt ttgaagaact tcttaagtgg tctcataaat
     8641 ttattgaagg taaaaagata gaagatgttt tgtatataaa gtctaaagaa aactataaag
     8701 taaatgtgat taagtcttta gaaattaagg aattggaaat agtgagaaaa caacagatac
     8761 caccgtacat gtagtattaa taatatataa aattaatatg taatactcgc aataggatat
     8821 aaatggtata tatggtaata atttatatta aaaaaacttt tataaagttt atatttgggt
     8881 ataaaagtat taattagaca ttaattagga ggaacttaaa tatgataaga gtatgtccaa
     8941 actgttcaaa tgtggatgta aataagctaa aaactttggt tggagaagaa aatgtaaaga
     9001 ctggttgtat aggtcagtgt agagcattta aaaaagaagc tgttggaata atagacaatg
     9061 agcttgaaat aaaggaaaca gaagcagagt tttttgaagc atgtaaagaa taaattttaa
     9121 ataaaatata aattataata aatagagcat ctaaattgta taagcattaa acaatttaga
     9181 tgctctattt ttatatatac atttaattat gagtatactt ttctattaaa tacaaataaa
     9241 tataactaat acatttattt attaaaaaaa tattgattta atatacctat agggggtata
     9301 atgaaaatat agatttctaa ataagtaatt tatcaatatt acatatcaaa ataaaacgtt
     9361 acaaattagc tgaaaatata ttgaaagaaa ggaagatttt attatgagtt caaataaagt
     9421 ggtagaaagt tataaaatta caggtatgac atgtgcagct tgtgcaaagg cagtagagag
     9481 agtaactaag aagatggatg gagtatatga ccaaaatgta aatatagcta ctgaaaaact
     9541 taaaatagaa tatgataatt ctaaggttaa ttttgatgat ataaagcagg ttgtagaaaa
     9601 agcaggatat ggaattataa aggaagaaag taataaaaaa attgatatga aaattgatgg
     9661 aatgacatgt gcagcttgtg caaaagctgt agagagagtg gtaaaaaaat tggatggagt
     9721 agaaagtatt agtgtgaata ttgcaacaga taaagctaat atagactatg acccatcaaa
     9781 agtaaaatta tctcaaataa aagcagctat tgaaaaagct ggatataaac caatagaaga
     9841 ggtaagaaat aaggttgatg tggatgaaga taaattgaga aaagaaagag aaatgaagtc
     9901 tttatttgtt aaatttatag ttgcaatagt atttgcagta cctctgtttt atatagcaat
     9961 gggacctatg ataataaagc ctatagggcc atggcctttg ccagaaatta taaatcctat
    10021 gacaaatact tttaattatg cattaattca gttaatttta gtaataccag taatgatagc
    10081 tggttataaa ttttatataa atgggtttaa atcacttttt tctttaagcc ctaatatgga
    10141 ttctttagtt gcaataggaa cattagccgc atttttatat agtctatata cgacactaca
    10201 aatagccaat ggacaaattc aaggtatgca ccatcatcag ttgtattatg aaagtgcagg
    10261 aataataata gcacttatct tacttggtaa atatttagaa tcaaaatcaa aaggaaaaac
    10321 atcagaagca attaaaaagc ttatggggct acaacctaaa actgcaatag tgttagttga
    10381 tggtaaagaa gttgaaactc ctatagaaga agtagagata ggagatatac tattagtaaa
    10441 accaggaact aaaatacccg tggatggagt tgtaatagaa ggatatacat cagttgatga
    10501 atcaatgctt acaggtgaaa gtataccagt agaaaagaat gttggaagta aggtgacagg
    10561 agctagtatt aataaaaatg gtgttattaa gtttaaggct gaaaaaattg gtggagatac
    10621 tgctcttgcc caaataataa agttagttga agatgcacaa ggtacaaaag cacctattgc
    10681 taaacttgcc gatactgtat ctggatattt tgtaccaata gtaattgcaa tagctgtagt
    10741 tgcatcatta ttgtggtttt taataggagg aaaagatata gtatttgtac ttactatatt
    10801 tatatcagta cttgtaattg cttgtccttg tgcattagga ttagcaacac caactgctat
    10861 aatggttggg actggaaagg gcgcagaaaa tggaatcttg ataaaaggtg gagaggcact
    10921 agaatctgca cataaagtaa atacagtgat atttgataag acaggtacta taacagaagg
    10981 aaagccaaaa gtaacagata tagtattaaa taataatgta aaagaagaat atcttatcaa
    11041 gattgcttca agtgcagaaa aaggttcaga acatcctctt ggagaagcaa tagttaagta
    11101 tggagaagag aagaatataa aatttgaaaa agttgataat tttaaagcta taccaggtgc
    11161 tggtatacaa gttactatta atgatgaaag tatattactt ggaaatagaa agcttatgaa
    11221 tgacaataat ataaagcttg gagatttaga agaaaaatca aatatattgg caagccaagg
    11281 taaaacacct atgtatatag ctgtagatgg aaatttatca ggtataatag cagttgcaga
    11341 tgtagtcaaa gaaagtagta aaaaagcaat agaaatactt catgatatgg gaataaaagt
    11401 tgcaatggta actggagata atgcgaaaac tgcaaatgct atagcaaatc aagtcggtat
    11461 agatatggtt ttggcagagg ttttaccaga agataagtca aaagaagttg aaaaactaca
    11521 aaatcaagga aagtttgttg caatggtagg ggatggaata aatgatgccc cagctcttgc
    11581 aaaagcagat atagggatag caataggaag tggaacggat gtagccattg aatctgcgga
    11641 tatagtactt atgaagagtg atttaattga tgttccaaca gcaattaaac ttagtcatga
    11701 aactataaaa aatataaaac aaaacctatt ttgggcattt ggatataata caattggtat
    11761 acctgtagca gcaggaatat tatatgtatt tggaggacct ttattaaacc caatgattgc
    11821 agcagcagca atgagcttaa gttcagtttc agtcgtatca aatgcactta gacttaaaaa
    11881 cttcaaggca tataagagag attagattaa taaattaaat aaaatgtatc caaaagagct
    11941 atctttaaat taaaaggatt aatttacaaa ttaagatagc tcttttttca tgtaaaattc
    12001 acaacaataa gataaaatat aagtaatata cgaaatttta aataaaatag aaaggattga
    12061 tagaatgaat tcgtcaatac ttgtaattga agatgattca aatatacaag agttaatatc
    12121 agaattttta agtgcagaag gttatcaagt tgatacagca aatgatgggt tggaaggtat
    12181 acaaaagttt aagcaaggaa gttatgattt agtaatatta gatataatga tgccaaactt
    12241 agatggttat ggagtttgta aaatgataag aaagtcatca agcgttccaa ttatattttt
    12301 aactgcttta aatgatgaag gagaccaact taaagggttt gacttagaat gtgatgatta
    12361 tataacaaaa ccattttcat ttaatcttct tataaaaaga gtagaagcaa ttttaaggcg
    12421 aagtaacaag actataaatg acaaatttat agtttttgaa aaattaaaat tagatttaaa
    12481 cacatatatt gcagagatag atggagaacc tatagaactt acactaaaag agtttaatat
    12541 attaaaagct ttgatagaaa agtatccaca agttataact agggaaggtc ttttggatag
    12601 tatatggggt tatgactatt atggagatac taggattgtt gatgcacata taaaaaatat
    12661 aagaaaaaaa atatcattac catatattaa aactgtaaaa ggaataggat atacacttga
    12721 gaaggatatt tgataagtgg gaaaagttaa gtataaagta taagttattt tcaataacga
    12781 catcattatt aatagcatta gctttaataa tatatctaat tttatatttt ttattgccat
    12841 catattatca tgagtataaa attgagtctc ttcaagaaag tttgaaatct ttagtagata
    12901 gttcaattca tttcgataca tatactttag aagaaagact ttattatatg gctaaagacc
    12961 aaaatttggc tattttatta aaagataacc aaggtaaaat tgtatatggt aagaatgaag
    13021 tagttatttt aagatatagt aagtacatga ttaatagtct tgaggatgag tatagaactt
    13081 ctatacctat atatactaaa gatgcaaaag atggtccata tactttagaa ctcgttatgc
    13141 cattacaacc aattgacgaa gcaaacgaag ttataagaaa actaatgcca tacatcatat
    13201 caatagcaat attgatagcg attattggag catatatata ttcaattgta ataacaaagc
    13261 ctcttataaa cataatagag agtgagagag aacaggaata tagaaggaag gattttgttg
    13321 ctacaatatc acatgaacta aaaactccaa taaccataat aagtggtcaa attgaaggta
    13381 tgatttatag tgtaggtaag tataaggata gggatactta tctgaaaaaa tcgtatgagt
    13441 gtacacaaga attaaaagat ttagtaaatg aaatgataga ggtttcaaaa tcagaaatac
    13501 ttgaaaaaga tttgaaattg gtatcaataa atataagtga attattaaat agacttgtaa
    13561 agagacaagt atttttaata gaagaaaaac atatgaaaac tattttaaaa atagaagaaa
    13621 atttggaaat taaagcagac caagagagaa taactaaggc aatcaataat ataataaata
    13681 atgccataaa atattctcct gaagaatcag agcttataat tagactctat gataaaaata
    13741 aacgaataag taaaaagact tctaatcaaa gagtggtttt agaaattgaa aatactggtg
    13801 taactatcga gaaacgttat ttagaagaaa tatttaatcc attttataga atagaaaaat
    13861 ctcgttcaag aaaaactgga ggaagtggac ttggacttta tatagtcagc caaattttta
    13921 aaagtcatgg gtttgattat agcataaaaa acaaagaaaa ctctgtagta ttcacagtag
    13981 aatttaaaaa ttaattactt attataaaga atatataact ttcaataata gttcatttta
    14041 aaatcacaaa aaattcataa tgaaactgta ttatataaat ataaagaagc ttaaaaccat
    14101 aaaatacacc aactataatg atgagatttc cttacatcct ccatataagt catcattata
    14161 gtaacccaac aattaagtta ctatgaattt aaaaaataac accgagcata taatctatgc
    14221 ttagtgttat ttttattttg aaataaaaaa tatttaattt attgaaatgt atagatttag
    14281 atagtttaaa ctatatttaa gacttaaaaa ttataattga tattacaaat aaatatatgt
    14341 actttgggaa aaataaatat aggaagtatt atatttacag aggaggtagt tttgatggat
    14401 agattaaaaa gagatatgaa aaatatgaaa gaagatttaa aagaaggtgt taaaaagaca
    14461 gcttatgtta tgaaaaatac agctgaagat ttgaaagatg atgcacaagg tgctatggta
    14521 aacttagaaa tgaaaaaaga tgagatgatg gaaagatatg aacaaaagaa attttctaaa
    14581 gaattagaga agcaaatgaa gaatgaaatg aaataagctt ttatttatga ataacaaatt
    14641 attgtattca tataaaaagt agccagtaat attaaatagt ttatttaagg aaatagactt
    14701 tctaaaaata actgtattag atttagaaag tctatttcaa attagtttta taaatagaca
    14761 taaggataaa gtagatgtta tagaactaga ttttaaaa
//
LOCUS       DAMJVI010000089        14748 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.094, whole genome shotgun sequence.
ACCESSION   DAMJVI010000089 DAMJVI010000000
VERSION     DAMJVI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14748
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.094"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            427..1338
                     /locus_tag="PC545_002822"
     CDS             427..1338
                     /locus_tag="PC545_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2457582.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMTKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNTINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKDEVFSRKIIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDDTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLRELSKIYSL"
     gene            1513..2010
                     /locus_tag="PC545_002823"
     CDS             1513..2010
                     /locus_tag="PC545_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2457583.1"
                     /translation="MNYEIVEINEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKNDKTIGLYTDYQGDFTSPYSFVACCEVNSNSDKEKNLEELNTNNTVGESII
                     SKVIPAGKYAKFVIVGGQKEVGDFWFEFWQMDFDRTYISDFEEYQCNTFDTKKQEVHI
                     YIGIK"
     gene            2754..3116
                     /locus_tag="PC545_002824"
     CDS             2754..3116
                     /locus_tag="PC545_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDF2457584.1"
                     /translation="MENKILIVDDAIFMRMIIKEALSKSGYNNLIEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVEAIKLGALDFI
                     VKPFKTETLVKVVDNALK"
     gene            3181..3798
                     /locus_tag="PC545_002825"
     CDS             3181..3798
                     /locus_tag="PC545_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HDF2457585.1"
                     /translation="MINYLELDDVHIDALREIGNIGSGNAITALASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEAF
                     LDEIEAIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFIENKFKMSDNYFTSHILMIPEMSSLREILVRLGLEV"
     gene            3795..4280
                     /locus_tag="PC545_002826"
     CDS             3795..4280
                     /locus_tag="PC545_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HDF2457586.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKCKFADTGINELIKSMENIGANRIYIKAKIAGGAQMFLGSSN
                     SVIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELLVDSINKGKKK
                     L"
     gene            4323..4841
                     /locus_tag="PC545_002827"
     CDS             4323..4841
                     /locus_tag="PC545_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HDF2457587.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFRVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMTTNCMYIVVTEIKDLVVGFV
                     VDRVDDIVSLEKSKISPPPRVTIEYSDYFVSGVGELEGKIITLLDLEKLLTDKDEVLI
                     DELINGFDCTIM"
     gene            4977..5936
                     /locus_tag="PC545_002828"
     CDS             4977..5936
                     /locus_tag="PC545_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDF2457588.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKTLTKDNFKVKKAFNAIEAKE
                     FIEEFIPDIILLDIILPDNSGFEFCKEVKSNARTADIPIIFITSKNTDTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTVISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEGAKILSERIRKEIKEFDFNYKEHKIKCTITLG
                     VVEVNSNISLEENISLADKAMYEGKNLGKDCSVVSTMKGLKKI"
     gene            5962..7665
                     /locus_tag="PC545_002829"
     CDS             5962..7665
                     /locus_tag="PC545_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDF2457589.1"
                     /translation="MKREVKLSKLAIVLLIIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQSIIFGSDYINSQEKMDSLI
                     NEFQEDIVTRSNSKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVGFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVRIAKETANALDLVVNGVEKTTNYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKESEVRESSW
                     V"
     gene            7926..10028
                     /locus_tag="PC545_002830"
     CDS             7926..10028
                     /locus_tag="PC545_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDF2457590.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEEYVSTLELSSSMKSKDNANLESKDEKEVIDSNFEYKED
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFIDFYPKDVETNAESTEYIKSYGFYIQ
                     FVAKEDKAIVYKTIEDNLYVKEYINISKKEIEMKNTSNNDAVENKLKDNNKVSKSYQN
                     NYTEDEIQEDVLNEKTDMEKDSLSEENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKATRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLAEPFMHLIRNAMDHAIEPIEERIAKGKEEVG
                     KIVLSAENLGGEAVISISDDGRGIMRDKILAKAKEQGLLKHQSNEYTDDEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKQDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEGNKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMNGNMIIIENEHKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            10041..10481
                     /locus_tag="PC545_002831"
     CDS             10041..10481
                     /locus_tag="PC545_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDF2457591.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPKADYNERTCIIITKVDEYQVGIIVDKVVDVIHI
                     ENLNLLETTNSNNTNINKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            10517..11320
                     /locus_tag="PC545_002832"
     CDS             10517..11320
                     /locus_tag="PC545_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDF2457592.1"
                     /translation="MIKLTDEEFIVLVNYVKKEYGIDLSKKRALIEGRLYNTMIEKKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSILPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSSTKYKYVKPAIYKR"
     gene            11360..11956
                     /locus_tag="PC545_002833"
     CDS             11360..11956
                     /locus_tag="PC545_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HDF2457593.1"
                     /translation="MERFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGYQMKLCTDKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            12062..12925
                     /locus_tag="PC545_002834"
     CDS             12062..12925
                     /locus_tag="PC545_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HDF2457594.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLRKDASI
                     NLNSNKEAVTVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSN
                     KIVERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSI
                     KVRYYTYSGGAHGFYQDIAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELE
                     KKDEENIGIYNFKGIKENQNFYLQDENLVVYFDLYDITPYAAGIPEFSINKALISSML
                     KPEYVDLFDLK"
     gene            12992..13981
                     /locus_tag="PC545_002835"
     CDS             12992..13981
                     /locus_tag="PC545_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDF2457595.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNISV
                     PSQDASMLFMPGYIEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGLGGQGEPQLEAMIEARNLDEVRVFSR
                     NKESREKFAEEMNLKLSKYNTKIVAVESSDEAINNADVIVLATPSKQPVLNGNLVKKG
                     ALISAVGSYMPDMQELCPNCLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGIDWKW"
     gene            14073..14684
                     /locus_tag="PC545_002836"
     CDS             14073..14684
                     /locus_tag="PC545_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2457596.1"
                     /translation="MKMVKKLILSTIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGISDALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANTLVKSYVTDEKNYDKKEKEVQDDMTK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         6038 a         1378 c         2733 g         4599 t
ORIGIN      
        1 gatgataata agtgaattga tatagaaata agtaaattaa ataagacgat aacaagtgag
       61 ataataataa atgaattgac atagcaataa gtaaattaag taagacgata acaaatgaga
      121 tgataataag taaattgata tagaaataag taaattaagt aagatgataa caagtgaggt
      181 aataataagt gaattgatat agaaataagt aaattaagta agacgataac aaatgagatg
      241 ataataagta aattgatata gtaataaata aattaagatg ataatatcca aagtgagaaa
      301 gataatagaa agtaaataaa atcatgtaag aataatttag ataagattct aagattttat
      361 ttatttaaaa attagtatga ttaatttaga ttaaataaat ttaataattt tagtaggtgg
      421 taatgaatga aaataaatag attaacagaa ataatagtaa ttctattaaa taaaaaatta
      481 gtaacagcta aagagttggc agatagattt gaagtttcta caagaaccat atatagagat
      541 atagaaactc tgtctatgtc gggagtacca gtatatatga cgaaaggcaa aggaggaggg
      601 atatctttaa tagaagaata ttctatagac aaggctatat tgtcaaaaaa agataaagag
      661 agtttaatag tggcactgaa aacattacag gcgactaagt atccagaaat aaattcagta
      721 gtaaataaaa ttggctcaat atttggagag cagaattttt caaactggat tgaaatagac
      781 tttactgaat ggggaagtaa ttttaatgaa gatgataaat ttacaaaaat aaaagaagct
      841 attttaaggc gtaatactat aaattttaat tatgtaaata gtttgtcaag ccagacaaac
      901 agaactgtcg aaccactgaa gcttatgtat aagagtaaga cttggtactt gtatggattt
      961 tgtaaactaa aagatgattt taggatattt agaattagta ggataaggaa tttatctata
     1021 aaggatgaag tattttcaag aaagataata gaagaagtat gtttgaatga ttctaaagta
     1081 attaaggaaa atactataac gttgaaattg aggtttaaag aaaagatgct ttttagagta
     1141 tttgatgatt ttaataagga tttaataact aaaaatgaag atgatacata tgatgtaatt
     1201 acagaatttc ctattggtga atggatttat ggatatattt tatcatttgg tgacaatgtt
     1261 gaagtacttg aacctaaaga tgttagggat aatgtaataa ctagactaag ggagttgtct
     1321 aaaatttatt cattataata taatgctaga ttaaagattt tattaaatat ataatatctt
     1381 gcatttagta tataatatct cactgagttt aattatatta aaaatatgac ataaaggtgt
     1441 cgtatattat aaattataat tgaattagtt caaaataaaa tatattaata aaaacaaagt
     1501 gaggtataaa gtatgaatta tgaaatagta gagataaatg agaaaatagt tataggaata
     1561 tcgaaggaga ctacaaataa agatggacaa gcggtaaatg atataggtga attgtggaaa
     1621 aaatttatgg gcaaagggat atataatgct ataaagggta aaaagaatga caagacaata
     1681 ggtctatata cagattacca gggtgatttt acaagtccgt atagctttgt tgcatgttgt
     1741 gaagtaaata gtaattctga taaagagaag aatttagaag aattaaacac taataatact
     1801 gttggagaaa gtattattag taaagttata ccagcaggta agtatgcaaa gtttgttata
     1861 gtaggaggtc aaaaagaagt aggagatttt tggtttgagt tttggcaaat ggattttgat
     1921 aggacttata taagtgattt tgaagagtat caatgtaata cttttgatac aaagaaacaa
     1981 gaagttcata tttatatagg aataaagtag atttgttatt actatattga atgaagtatt
     2041 gagtatagta catttattat agatagtttt attatttata actataagca gtaaatctgt
     2101 aaaagattta ctgctttttt agtaattaat attatcagta tcactttgaa tcttcagaca
     2161 tattcttcat ttaatattgt ttattgataa atgatacttg taaatataat gatttgttat
     2221 ttaatttaaa atatgaaaaa aagtattata aacacattag aatacttctg gatttatgag
     2281 tatacaattt gaaaaatata aaaaataatg tataaagcta aatctaattt aatcgataat
     2341 acatatagag aaatataatt aagtaaataa acaactatct attagatatt gagaaagtca
     2401 aatcctatat gaatctacag aaatgatttt aatgaatctg aaaaattttt ttatacaaac
     2461 ataaaaaagg tataaagttg ttttattata atttaattac ttttgataac tttttatgtt
     2521 ttatacaatt atatttttaa taactcgtca aaacgccaaa catatattac aatagatata
     2581 taagtttttt tgttgacaaa ttatgttaag agaattaagt ataatatggc ataaataatt
     2641 acttttattt atcaatgaaa tgtatttatt gtgtagcgaa aaatagtcag atagtataga
     2701 caataactga agataaaagc gtttattgtt gtacatatgg aggagttagt tttatggaaa
     2761 ataaaatttt gatagtagat gatgctattt ttatgagaat gataattaaa gaagctctga
     2821 gtaaaagtgg ctataataat ttaatagaag caaatgatgg agaagaagcg tgtaatatat
     2881 ttaaatatga aaagcctgat ttgatgttgc ttgatattac aatgccaaaa aaagatggtt
     2941 tagaggtgct aagagaaata aaaaagttgg attcaagtgc taaggttgta atgtgctctg
     3001 caataggaca agagaatatg attgtagaag ccataaaact tggagcgtta gattttatag
     3061 tgaagccctt caaaacagaa acattagtta aagttgtaga taatgcttta aaataaaagt
     3121 gtaaatatac actaatacaa tgaaaagcaa ataaatgcaa taataaaggg aggggtttat
     3181 attataaatt atttagaact tgatgatgtt catatagatg ctcttagaga aattggtaat
     3241 ataggctctg ggaatgcaat tactgcactt gcatccatga taaatagcaa tgtagaagta
     3301 ttaataccta tggtcaagat acttgaatat aatgaagcga caaatttgct tggtggtcca
     3361 gaaaacaaag tagtatgtat tttactcgat atgaaaggtg atatcaatgg aatgtttatg
     3421 tttttgttgg atgaatcaat aactcaactt atgctaagtt ctctttttaa taaagaagaa
     3481 gcttttttag atgagattga ggcaattgaa atttcagcaa ttaaggaaat aggaaatatt
     3541 atggcaagtt catatgtaaa tgctattgca tctatgttaa acatgactat aagtgtttca
     3601 attccagata tatgtattga tatggttgga gctgttctaa atgtacctat gataagattt
     3661 tcagatgttg gtgacaaagt cttatttata gaaaataaat ttaaaatgag tgataattat
     3721 tttactagtc acattttgat gattccagaa atgagctcac ttagagaaat actcgtgagg
     3781 ttgggattag aagtatgaat agagagatag tagtgaacat tgcagatatg aagatagcct
     3841 atagaccaaa tgttttagtt acttatgcac ttggttcttg tgtaggagta tgtttgatag
     3901 ataaagtagc tggaattggt ggaatgttac acgttatgtt gccatatagt aaagatgcta
     3961 ttaacattga aaataagtgt aaatttgctg acacaggaat aaatgagctc attaaatcta
     4021 tggagaatat aggtgcaaat agaatatata taaaagcaaa aattgctgga ggtgctcaga
     4081 tgtttttagg aagtagcaat tcagtaattg caagtatagg acataggaat gttgaggcag
     4141 taaaaaaaac tcttttagga ttaaatattc caatattagc tgaggacaca ggtatgaatt
     4201 atggtagaac aatacgtttt tacactgaga cgggtgaatt acttgtagat tcaataaata
     4261 aaggtaagaa gaaattataa tatatatttc atagaaagtg tattttagga gggaggtatt
     4321 aaatgagatt tccagatgac ccatatttag attatggact aattgatgaa ggaattaagt
     4381 atctaaagtt tagagtagat gagcaagact ttggggttga acttgaaaag gtaatagaaa
     4441 ttactggaaa tcaagaaatt atatttattc cagaactacc tacatattct aaaggcataa
     4501 taaatctaag aggaaaaatc attccgataa tagacatgcg tttgagactt aaaaaagatg
     4561 aattaatgac tacaaattgc atgtatatag tagtaactga aataaaggac ttggtagttg
     4621 gatttgtggt tgatagagtt gatgatatag taagcttaga aaaaagtaaa atatcacctc
     4681 caccaagagt tactatagaa tattcggatt attttgtaag tggagttgga gaattagaag
     4741 gtaaaataat tactttacta gatttagaga aacttttgac agataaagat gaagttttaa
     4801 tagatgaatt aatcaatggg tttgattgca ctatcatgta acatagggat gtagttggag
     4861 tgttattata aaaagattgt gatgagaaca tttaagaata tagtggtata attattaagt
     4921 ataaaaattt taaatcatta aaatttgaat atattatgtt taagagaggt ttgtatatga
     4981 aaaataagca acagggaaca attttagtcg ttgatgatag tgttctgatg tgtgacttaa
     5041 tagataaaac acttacaaaa gataatttta aggttaaaaa agcatttaat gcaatagagg
     5101 caaaagaatt tattgaagaa tttataccag acattatttt gcttgatata attttaccag
     5161 ataactctgg ttttgagttt tgtaaggaag taaaatctaa tgctcgtaca gcagatatac
     5221 ctataatttt tataacttca aaaaatacag atacagacat agtaaaagga tttggtgtag
     5281 gagcaattga ctatatggtg aaacctttta gtatgacaga attaaaagct cgtgttatag
     5341 ctcatcttga agtaaagaaa tctcaagata aattaaaaaa gataaataat gaactggaat
     5401 cttctcttga aaaattgaat agactcgttg taagagatta tcttacaggt ctttacaata
     5461 ggcgacatat aattaatcag cttatggagc aaagaagaat taataaatat agtgaaacat
     5521 caatatccct tatccttggt gatattgatg actttaaggt tatcaatgat acttatgggc
     5581 atgaagcagg ggatttcgtg ttaacagtaa tatcaagtat aataaaaaag aattgtagac
     5641 aaattgatac tgtatcaaga tggggtggag aagagttttt gataatgctc acaaatactc
     5701 caatagaagg tgctaagata ctttcagaaa gaatccgtaa agagataaag gagtttgatt
     5761 tcaactataa agaacataaa ataaagtgca ctattacatt aggagtagta gaagtaaact
     5821 caaatatatc attagaagaa aacatatcct tagcagataa agcaatgtat gagggtaaaa
     5881 acttaggaaa agattgtagc gtagtgagta caatgaaagg attaaaaaag atataaactt
     5941 actttatgaa ggagtacaga tatgaaaaga gaagtaaagc tttctaaatt agcaattgta
     6001 ttgctgatta ttatagtatt ggctgttcta gcagggattt tttcttctat ttcatcttat
     6061 aaagcatttg aaaaagtaga aaaaagtatt aatgatatga ataatttgag gactcaaagt
     6121 cagtatataa aagatacaac tagagagata agtgatgtat taagaagata tgtatttaca
     6181 ggagaaaaaa agaataaaga tatttatgaa aaagaattat acggaaaaac aagagaatca
     6241 gctttaaaca aattagaaaa aattggtctt acaaagagcg agtcaaataa aatcttagaa
     6301 gcaaaagaaa tatcagattc tttagtacca acagaacaaa gtgcaataaa ggcagtcgaa
     6361 agtaatgata agagtaaagc tcaaagtata atttttggta gtgactatat aaactcccaa
     6421 gagaaaatgg attctttaat aaatgaattt caagaggata tagttacccg ttcaaattcc
     6481 aaaattgtag aggtaaaaaa agaattacaa agtctaatat taagaacaat atttatactt
     6541 tcccttttaa taatattgac aataatagaa tatacagtaa taaaattaaa aatagtgatt
     6601 cctattaata aagtagaaaa acacttttca agaattgcat ctggggactt aagaacaagt
     6661 atagacgttg aagaaagtaa atctgaaata gggctattag tgagttctgt caaaaagatg
     6721 caaagtatgc ttttaaatta catagacctt atagacagta cattaactag tattgcaaaa
     6781 ggagatttaa gagtagagat agaccaagaa tttataggtg attttaaatc aattaaaata
     6841 tctttagaaa gtataataaa ctcttttaat ggtgattttt ctgaaataaa tttatcagca
     6901 gagcaagttg caagtgcttc agaacaagtt gcagcaggtg cacaagctct ttcacagggt
     6961 gctactgaac aagctagttc agtagaagaa ttagcttcca cgattaatga gatatcagat
     7021 ggaattaaga agtgtgcaga tagtgctagt cttgcatgtg aggtatctga ccaatctgca
     7081 tcacaagttg gttttggaag tagatgtatg gatgatatga tgacatcaat gggtgaaata
     7141 agtgaaaaat cgagtgaaat atcaaaaata atcaaaacaa tagatgagat agcattccaa
     7201 actaatattc ttgcattaaa tgcagctgtt gaagcagcaa gagcaggtca atatggtaga
     7261 ggatttgcag ttgtagcaga tgaagttagg tctttggctg gtaaaagtgc aaaagcagct
     7321 caaaatacag ctattctcat agaagaagct attaaaacag tagaaaatgg tgtcagaatt
     7381 gctaaagaga ctgcaaatgc attggacttg gtagtaaatg gtgtagaaaa aactactaat
     7441 tatattaatg gtatttcttc ggctgtacat agtcaagaag tagcaattac acagatttta
     7501 tctggaatag agcaaatttc catagtagtc caaatgaact cagcaacagc agaggaaagt
     7561 gcagcagcct cagaagagat gtctgctcaa gctcaaattt taaaagaatt agtagaaaaa
     7621 tttcatctaa aggaaagtga agtaagagaa tcatcatggg tttaaattat gaggtgaata
     7681 ttgaagtaag agaattatca tgtatttaaa ttatgagatg tatatatatt gaaataagag
     7741 aattatcatg tatttaaatt atgagatgta tatatattga agtaagagaa taattgtgta
     7801 tttaaattat gaggtacata tagtgagata agagaatcat caaggattta aattatgaga
     7861 tgcatatata atagaaaatt atttgtaggt cgtaggtgat aaatgtgaat tatattgaac
     7921 cgagtatgag acaaatgtta gatatgttta tatttgagac aagtttatta ctcgaacaat
     7981 tagatgaaat tatgattcaa atagaaaaag agaaagtatt tactcataac aacattaatg
     8041 aaacttttag aataatgcat acgataaaag gttcagcatc tatgatgggg ttagacaata
     8101 tatctgcttt agcacatagt gtagaagact tgttttatgc tatacgtgaa ggtaaaatag
     8161 aagttttaga caatgaccta ttatttgatt tagtctttca atcattagat tttataagaa
     8221 gagatataga tgagttacaa tcaagaggtt gtatatcttc tgattactct aattttattg
     8281 aaggaattga agagtatgtg agtactttgg aattatcaag ttctatgaaa tcaaaggata
     8341 atgcaaattt agaaagtaaa gacgaaaaag aagtaatcga ttcaaatttt gaatacaaag
     8401 aagactctga tgtagtaaaa ataatctttg aacaaggttg catgatggaa aatgtgagag
     8461 ctttcatgct tgtatttgaa cttagagatt actgtgaatt tatagatttt tatccaaaag
     8521 atgtagaaac aaatgcagag tctacagagt atattaaaag ttatgggttt tatatacaat
     8581 ttgtagctaa agaggataag gcaatagttt ataaaactat agaagataat ctctatgtaa
     8641 aagaatacat aaatattagt aaaaaagaaa tagaaatgaa aaatacatca aacaatgatg
     8701 cagtagaaaa taaattaaaa gataataata aagtaagtaa aagttatcaa aataactata
     8761 cagaagatga gatacaagaa gatgttttaa atgaaaaaac tgatatggaa aaagatagtc
     8821 taagtgaaga aaatgaacat ttaccaataa gtaagcagag tattatgagt gtaaatttag
     8881 aaaaattgga cttacttcaa aatatagttg gagaaattgt aattatggaa tcaatggttg
     8941 taaatagcgt aaattctaca ggtgttgata tagataattt caataaggca acaagacaat
     9001 tacacaagct tacagatgaa ttgcaagata tagtaatgtc tatgcgtatg attcaaattg
     9061 ctggtgtatt tcaaaaaatg catagaattg tacgagatat gaataaaaaa ttagataaaa
     9121 atgtagagtt aataactata ggagcagaga cagaggtaga taagagtgta attgataatt
     9181 tagcagaacc ttttatgcac cttataagaa atgctatgga ccatgcaata gagcctattg
     9241 aggagcgtat agcgaaaggt aaagaagaag tgggaaagat agttttatct gctgaaaatc
     9301 taggcggaga agctgtgata agtatttcag atgatggtag aggcattatg agagataaga
     9361 ttttggcaaa agctaaggaa caaggtcttc ttaaacatca aagtaatgag tatactgatg
     9421 acgaggtcaa ttctttgatt atgactccag gcttttctac aaaagaatct ataactgaat
     9481 tttctggaag aggagtaggc atggacattg ttcgtaaaag tattgaaaaa gttggaggtt
     9541 caattgaagt aaaaagtaaa caagatgaag ggacaacctt tactataaaa ataccattaa
     9601 ctctttctat agtagagggt atgaagttta aagttggaga ttcactattt actttaccaa
     9661 tatcatccat aagaagaaca tttaaactta cagacttaaa tcaaataatt acagaaggaa
     9721 ataagacaga gttaattaca atatatggag catgtattcc cattataagg ttacataaaa
     9781 tatataatat tcaaacaaaa gtaaccaatc tgatgaatgg taatatgata attattgaaa
     9841 atgagcataa agcagtatgc atttttgttg atgaaataat aggtaatcag caagttgttg
     9901 tgaaaccatt tccagtttat tttaaccgat ttaatataaa aaataagggt ttatcgggat
     9961 gtacaatact tggggatgga agtataagtt taattataga tgcagatagc attatagaaa
    10021 tatattaggg gatttaaagt atggctaaag atgaaattat taaacaagta ttaacatttt
    10081 atgtcaatga tgttatttat ggaatagaat tagaaaatgt gattgaaacc ataagatttc
    10141 aatcaataac gtatgtacca tgtcttccaa catatataag tggagttatt aatttgagag
    10201 gaagaataat accagttatt aatatgcata taaagtacaa tcttcctaaa gctgattata
    10261 atgagagaac ttgtataata attacaaagg ttgatgagta tcaagttgga attattgtag
    10321 ataaagttgt tgatgttatt catatagaaa atctaaattt actagagacg actaattcta
    10381 acaacactaa tattaataaa gatataaaag agattgcgaa agttgaagat aattctatat
    10441 taattttaga tattcgaaag tttttgatta atagcatata gttagttctg ataaattagc
    10501 aggaggaagt ttttgcatga taaaactcac agatgaagaa tttatagttc ttgtaaacta
    10561 tgtaaaaaaa gaatatggta ttgatttaag taaaaaaaga gcgttaattg aaggtcgctt
    10621 atacaataca atgatagaga aaaaacttag ttcttttagt caatatatga atctattatt
    10681 taaagataag acaggaaatg aagctataaa cctaataaat agattgagta ctaaccacac
    10741 attttttatg agagaacctc aacattttga gtttatacag aatagtatac ttcctttttg
    10801 ggaagaaaat aataaaataa gaaacataaa tatatggagt gcaggatgtt ctagtggaga
    10861 agaagcgtat tcaatagcta tgactttgga tgattatttt ggatataata aagagttgtg
    10921 gaacattaaa atcatagcca cagatatttc aactaactca cttgaaaaag ctaaaagagg
    10981 tatatacata gaatcaaatg ttaacaatgt tcctgagtta tggaagaaga agtattttat
    11041 agacaacaag gatggtacat ttaaaatttg tgataagatt agaaaaagtg taatttttaa
    11101 gccgtttaat ttaatgaatg atttctcata tcaacatttt gatttgattt tttgtagaaa
    11161 tgttatgatt tattttgatt taaaaacaag agaagaactt ataaataaat tttataatgc
    11221 taccaaaaaa gatagttttt tgtttgtagg acatgcagaa gtaatcaaca gaagtagtac
    11281 taaatacaaa tatgtaaaac ctgctatata taaacgataa aaaggtattt ttgatatatg
    11341 attttttgga ggcggtaatg tggaacgttt taataatatg gttattgcta taggcgcatc
    11401 tgtaggtggt acagaagcaa tacttgaaat tataaaagac ctaccaaaat caacaccagg
    11461 gattgttgta gttcaacata tgccagctat atttacttat atgtatgcac aacggcttga
    11521 caagcagtgt attatgaatg taagagaagc taagaataat gatagagtcg aacaaggaaa
    11581 tgtgttaatt gcacctgggg gttaccagat gaaattgtgt acagataaac aaggatatta
    11641 tgtaacttgt gaaaagggag aaagaataag tggtcactgt ccatcagtag acgttttgtt
    11701 tgattcagta gcagaagtag caggtaaaaa ttcaatagga ataatactta caggtatggg
    11761 ttatgatgga gcaaatggac ttttaaaaat gaaagaaaat ggttcattta caattggaca
    11821 ggatgaaaat tcttgtatag tatatggaat gccaatggta gcttttgata gaggttctgt
    11881 tatgttacaa ttacctcttg gtgaaatttc aaactgtttg ataagacata ttaatatttt
    11941 aaaacaaaaa gattaatttt ttataaataa aaattaatct tttattaaat tatatggata
    12001 aaaaacccta gaaactccaa atataataag agagatttat atttaggagg aaaatgataa
    12061 tatgaaattt tttaaagtcg atacaaaagc taaagtttta agatatgtat ttgttttaac
    12121 attgactata atggctgttt tggcaattaa acatgactat aaatataata atttaaggaa
    12181 agatgcttca attaatttaa atagtaataa ggaagcagtg actgttatga gtaaccagtc
    12241 attaccaaag ggtaatacaa atagtaaaca aactttatca gtaagtacaa taggaagtaa
    12301 aacaatagtg aaaaatgatg agtatttaga aagtactata aacatgcctg taataacaaa
    12361 ttcaaataaa attgttgaaa ggtctacaaa tgataagata aaaaatgaca tatttgaatt
    12421 ttataataaa tcatataaag aagcaaagca gtatcttaag gataatcctg acgagaaaaa
    12481 taagtttgtt gcaaatgtag actttgaact taaaaagaat actgattctg ctcttagtat
    12541 aaaagtgaga tattatactt atagtggtgg agcacatgga ttttatcaag atatagcata
    12601 taatgtggat atgagaactg gaaaattttt agaattaatg gacttgttta aggataatac
    12661 taaatataaa gaagttatag atgaagaaat taaaagacaa atagctgaac ttgaaaaaaa
    12721 ggatgaagaa aatatcggaa tatataattt taaaggtata aaagagaatc aaaactttta
    12781 tttgcaagat gagaatctag ttgtatattt tgatttatat gatataactc catatgcagc
    12841 aggaatacca gaattttcaa taaataaagc gttgataagt tctatgctaa aaccagagta
    12901 tgtagattta tttgatttga aatagaagat taaattaaat gttatgctat aatatttagc
    12961 atagagactt attaaaattg ggggtataaa aatgctactt ttaaaaaaag acgacataaa
    13021 aaaagtattt acaatgagag atgcaattga agctgataag gaagctttta gaatttattg
    13081 tgaaggaaag agtgtaaatc ctttgagaac caatatatct gttccaagtc aagatgctag
    13141 tatgttattt atgccaggat acatagaaga attaggctgt gggggaataa aaattgtatc
    13201 tgtatttcct aaaaatgctc aaaaaggcaa accagtaatt ccagcaacag tactattatt
    13261 agatggagaa actggagaag ttagtgcagt attggatggc acttatgtaa ctcagattcg
    13321 tactggagca gcctctggag cagctataga cgtattggct aaaaaagatt ctaagattgg
    13381 agctttgata ggtctaggag gacaaggtga gccacaactt gaagccatga tagaagctag
    13441 aaatttggat gaggtaagag tttttagtag aaacaaggaa agtagagaaa aatttgcaga
    13501 agaaatgaac ttaaaattaa gtaaatataa tactaaaata gttgcagttg aatcatctga
    13561 tgaagccata aataacgcag atgtgatagt tttagctact ccatcaaaac aaccagtact
    13621 taatggaaac ttagtgaaaa aaggagcttt aataagtgca gtaggttcat atatgccaga
    13681 tatgcaagag ttgtgcccaa actgtttaac tagagcgtct aagatatttt ttgaatcaac
    13741 agatgcagtt ttatctgagt caggagatat attaattcca ttaaaagaag gtaagataag
    13801 taaagatgat tttagtggtg atttagggaa tgttataaac ggaactattc ctggaagaga
    13861 agatgatgat gagataattg tatttaaaac tgttgggata ggtgtacaag atgtcgtaac
    13921 agcaaaaaga atatacgata aagcaaaaga aaacggtatt ggaatagact ggaagtggta
    13981 aaatattcaa aataattcta tagttagaca aatttatgtt ataatgttga taaataaatt
    14041 ttttattatt ttaattgagg gaggatgttt gtatgaaaat ggtaaaaaag ttaatattat
    14101 ctacgattat ggctctagtt ttagttctaa ctatagggtg ttctaatgca gaagaaaaag
    14161 taaagtcgaa ttttgaagct aatttagctg cattagaaat gtataagtat gatgaaaaag
    14221 gaatttctga tgcattaaca gaagctgaat ataaacagat tcaaacttat aacagcaagg
    14281 acaaggtttc aatgattgct caattgacac ctctttatgg taaagaaaat gctgaaaaga
    14341 tatacgaaac aattgttaaa ttaattcaag gtgttgaaaa gacagtaaca gtggaatctt
    14401 ctagtaagga taagataact ttgaaagttg taagtaaagc tttagatact agtaaagtaa
    14461 tgagtgaagc gaacacttta gtaaaatctt atgtaactga tgaaaagaat tacgataaaa
    14521 aagaaaaaga agttcaagat gatatgacaa aaatgattat agacctttac aataagaaac
    14581 caactaagga agttacaact atgattacat atactaaaaa agatggtaaa tggcaaccac
    14641 ctagtaatat acaaaagtta tgtgaaggaa tatatacaat ttaatttatt ttaaagacac
    14701 aaaactatct tttaagcata taataaaatg tttggaagat agtttatt
//
LOCUS       DAMJVI010000090        14188 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.095, whole genome shotgun sequence.
ACCESSION   DAMJVI010000090 DAMJVI010000000
VERSION     DAMJVI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.095"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            497..1390
                     /locus_tag="PC545_002837"
     CDS             497..1390
                     /locus_tag="PC545_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HDF2457597.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELRNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            1390..2784
                     /gene="gltA"
                     /locus_tag="PC545_002838"
     CDS             1390..2784
                     /gene="gltA"
                     /locus_tag="PC545_002838"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HDF2457598.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            3150..5369
                     /locus_tag="PC545_002839"
     CDS             3150..5369
                     /locus_tag="PC545_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2457599.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIEASKRELENYLEVNISLLKALSQDDRFSNGETSLIEKGKLLSPYQKEYNLFMIGI
                     TDTKGNASSTYRENVNSIKDKPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIVKN
                     NEMIGTIFASFYFQDVNNLISRSNFDDSIKFLMIDKDYTIISHPNKKYVNDKSKMLDL
                     EGNIIGTTKSEILKNINKKCQGDFLSWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFK
                     NLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACNTEDELLNRLSKINKEICNLNTTNSSKYKIVMSSGIYKITKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEDTRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LDDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLKEIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            5516..6262
                     /locus_tag="PC545_002840"
     CDS             5516..6262
                     /locus_tag="PC545_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457600.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIEKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVVLDGYDVSKLKGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDMNIQTKSISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTRINKEQKVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            6262..8142
                     /locus_tag="PC545_002841"
     CDS             6262..8142
                     /locus_tag="PC545_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457601.1"
                     /translation="MDFRKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     TKSGMQSGSTAFMALMTSELIIFGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTEFYFPVKAMLLTIVS
                     FMILFLITGPMSLKLLSKKSILELLVGTKKPKKEIKSSKLFGIIGVICLILGYAMILF
                     GNKLKINGGMYTTALLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLIYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGEASKNNFPMVYSYFSEHNNEKEYEE
                     LQQIREAIKGYDYKELSFPVLKYNREVLLSVKNYNKLSKELGLDKISSKLEKYEGIII
                     PSSNSQEHINSLKQLKNYSIKNLNIEVKKSKNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKINFHGFDYKNWQSSAKITENLKEKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEIEDNVKYKKLSKIGLSFKNMNKIVSIEIGTLF
                     FIPYFIAIINSIISLLFLNQVLNNSFKLENLGILLLISSIYSLYFYLLKKNYTRKVWF
                     FD"
     gene            8261..9652
                     /locus_tag="PC545_002842"
     CDS             8261..9652
                     /locus_tag="PC545_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457602.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAILTIGYYIS
                     GKSNLRFSKSNLKLDKKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLVVATIILCMGMV
                     VVMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            9759..10799
                     /locus_tag="PC545_002843"
     CDS             9759..10799
                     /locus_tag="PC545_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2457603.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISTDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDDSL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            11288..11941
                     /locus_tag="PC545_002844"
     CDS             11288..11941
                     /locus_tag="PC545_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDF2457604.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFMEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVNASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            12017..12226
                     /locus_tag="PC545_002845"
     CDS             12017..12226
                     /locus_tag="PC545_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457605.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNIVMIDDGTGTLKQAVF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(12366..12932)
                     /locus_tag="PC545_002846"
     CDS             complement(12366..12932)
                     /locus_tag="PC545_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDF2457606.1"
                     /translation="MMNNELNTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNIPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDITKK"
     gene            13093..13746
                     /locus_tag="PC545_002847"
     CDS             13093..13746
                     /locus_tag="PC545_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDF2457607.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVTLQETSLL
                     MIVSVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(13904..14080)
                     /locus_tag="PC545_002848"
     CDS             complement(13904..14080)
                     /locus_tag="PC545_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457608.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         5561 a         1402 c         2380 g         4845 t
ORIGIN      
        1 aaattcaaat aattaatgaa tattgaaaat tttagatata taataaaata agagtatatt
       61 ttatacaata tgaaagctgg tattgcaata gattaaagcc ttatacacgt ttagttttta
      121 aagaaattag tgttattatg aaaacctaag ttaatttatt aaataaataa ttgaataaaa
      181 aaattttatt atgtatttat aaaatttttg ttacaaattt ggcataatat agtatacttt
      241 aagtgtgtat taggttaaat ctataacaaa ttagacgtat gatatagact tagtgtttat
      301 tggcatttac aagaaatttg ttaatatcaa ctatttgtag tttgggcagt tataattatt
      361 atagggggga tgtccaatac aaaacaatca accaagaact aatattatat tataaagagt
      421 tatagttttg tgtgtttatg tttttaatta aatttaaagt gggaaaaata tatttgttag
      481 aatggagaat agcgagatga gtaataaaat agttagtaag agacaattaa cagatagtat
      541 ttatttaatg gaaatagagg cacctagagt tgcaaaatct tctcaaccag gtcaatttat
      601 aataattaaa aatgatgaaa aaggagagag gattcctctt acaatagctg actatgatag
      661 agaaaaagga acagtaacta ttgtattcca aacagttggt gcttcaacta aaaagttagc
      721 aatgtttgaa gaaaacgatt ttgtaatgga ttttgtaggg ccattaggac aagcaagtga
      781 atttatacat gaagatatag aagaattaag aaataaaaag atattatttg tagctggtgg
      841 agttggttct gctccagttt atccacaagt taaatggttt aaagagcatg gattagatgt
      901 agatgtaata attggtgcaa gaactaaaga attaattata ttagaagatg atatgaaaaa
      961 agtagctaaa aatgtatatg tatcaacaga tgatggtaca tatggattta atggaagagt
     1021 tacagaccta ttaaaagatt tagttgataa tcaaggtaaa aaatacgacc aagcaatagt
     1081 aattggacca atgataatga tgaaatttat gtgccaatta actaaagaac taaatatacc
     1141 tactatagta agtttaaata caataatgat agatggaaca ggtatgtgtg gtggttgtag
     1201 agttagtgtt ggaaatgaaa ctaagtttgc atgtgtagat ggtcctgaat ttgatggtca
     1261 tttagttgat tttgatcaag caatgagaag acagtctatg tacaagactc aagagggaag
     1321 agctatgcta aaacttgaag aaggcgatag tcatcatcat agtaattgtg gatgtggagg
     1381 taacaaataa tggatgctaa aaaagtaaaa gtaccagtta gagaacaaga acctgctgtg
     1441 agagcaacaa attttgatga agtatgctta gggtataata aagaagaagc tatggcagaa
     1501 gctaatagat gtttggcttg taaaaaacca aaatgtgtgg gtggatgtcc agtaggaatc
     1561 gacataccag ggtttataac aaaaattaaa gaagatgata tagaaggtgc tgctaaagta
     1621 atagctaaga gcagttcatt accagctgtt tgtggtagag tatgcccaca agaaagtcaa
     1681 tgtgaaggtg tatgtatact tggaataaaa tcagatgctg tatcaatagg taaattagaa
     1741 agatttgtag cagattggtc aaaagaaaat gacataaatt tatctgatac agagcctaag
     1801 aaaaatcaaa aagtagcagt aataggaagt ggtccagcag gtctagcttg tgctggtgat
     1861 ttagctaaaa aaggctatga tgtaacaatc tttgaagcaa tgcatgagcc aggtggagtt
     1921 ttaacttacg gaatacctga gtttagactt ccaaaacaag ctgttgttca acctgaaata
     1981 gacaatataa gaaaattagg tgtaaaaata gaaactaatg taattgttgg taaaacaatt
     2041 acagtagatg aacttataga agatgaagga tttgaagcta tattcatagg ttcaggagct
     2101 ggtcttccaa tgtttatgaa tataccaggg gaaaatgcaa atggagtatt ttctgcaaat
     2161 gagtttttaa ctagagtaaa cttaatgaag gcttatagag atgattatga tacacctatt
     2221 agttctggta aaaaagttgc tgtagtaggt ggtggaaatg ttgctatgga cgctgctaga
     2281 actgcattaa gattaggttc agaatcatat atagtttata gaagaagtga aaaagagctt
     2341 ccagcaagag ctgaagaagt tcatcatgca aaagaagaag gcattatatt taatacatta
     2401 acaaatccta aggaaatttt agtagatgaa aatggatatg ttaaaggtat ggtttgtata
     2461 agaatggaat taggagagcc agatgattct ggaagaagaa gacctataga aatagaaggt
     2521 tctgaatttg tacttgatgt agacacagtt ataatgtcac ttggaacaag cccaaatcca
     2581 ctaatatctt ctacaacaaa gagtcttgat ataaataaga agagatgctt aataacagat
     2641 gaaaatggtc agacttctaa agagggtgta tttgcaggtg gagatgctgt tactggagca
     2701 gcaacagtaa tatctgcaat gggtgctggt aaaacagcag cagcatcaat agatgaatat
     2761 ttaaaagcaa aagttaatgc ttaatttgta aattaaagag agggtgtctc aaaatgaact
     2821 ttttagttca tgaggcactc tttttgtaca aaatcaaata tgttttaatc atttcaacaa
     2881 tgaaaatttc attcctgtag aaagtctaac ttttaggtct tagtacaatc tctattttga
     2941 tgtagccact tttttagatg gtttaataat ttatcagtta ttattgtaat atttgatact
     3001 aagtaacata actttgtcta actatgataa aatagttata caataaaggt cttgtaagga
     3061 gtgaataatg tgtggctaat aaaatcagat ttgtcaaatc taacattaaa taatagaaaa
     3121 ataaattgtg taattaatgg tattttaata tgaaaaaaag agaaaaagta tttgatgggc
     3181 tagtttttaa gcatatatta atcttattaa tactattagt tttgattgat ttaacaagta
     3241 ccatcatata tgcaagtaac tctatggaaa ttacttctag aaatatgatt gaggcatcaa
     3301 aaagagaact tgaaaactat ttagaagtaa acatatcttt attaaaagct cttagtcaag
     3361 atgatagatt ttctaatggt gaaacatcac taattgaaaa gggaaaatta ttaagtccat
     3421 atcaaaagga atataatctt tttatgattg gtataacaga tacaaaagga aatgcttcaa
     3481 gtacatatag ggaaaatgtt aactcaataa aagataagcc tagttttgag aaagcaatta
     3541 aaacaaagca agtagtagtt tcagatattg aagtcagtaa tgtaacaggt gacaaagtat
     3601 ttatcatata tgtaccaatt gtaaaaaata atgagatgat tgggaccatt tttgcttctt
     3661 tttattttca agatgtaaat aatttaataa gtagaagcaa ttttgatgat tctattaaat
     3721 ttctaatgat agataaagat tatactatta tttctcatcc aaacaaaaaa tatgtaaatg
     3781 ataaaagtaa gatgcttgat ttagaaggga atattatagg aaccactaaa agtgaaatct
     3841 taaaaaatat aaataaaaaa tgtcaaggtg attttttatc ttgggataat tggagattat
     3901 ataatgtaaa atatacgaac ataaagtgga ctaattggac tttagtatca aaatgtaata
     3961 tatttaagaa ctttaaaaat ctaatagtta attttatgat aaagttgtat ttttatatag
     4021 taatatttat gatattgtgg aaattaagta atgctaaatt aatagaacaa cttaaaaaat
     4081 tggcttatta tgattcttta agtggaataa aaaataaaga aaagtttaga aaagattcta
     4141 tgtatatttt aaagaattat tatcaagata atttttactt agtacaattg gatgtcaata
     4201 aatttaaata tataaatgag atgtttggat atgctgaagg gaataagata ttaattcata
     4261 tatcacaggt tttaaataat aatacaaata aatatgaaat ttgtgctagg atggataatg
     4321 accattttat attgttgatt gcttgtaaca ctgaagatga acttttaaat agattatcta
     4381 aaataaataa agaaatttgt aacttaaata ctactaactc atctaagtat aaaattgtta
     4441 tgtcaagtgg aatatataag attactaaaa aggatgatat taaaaagatt gacttgttaa
     4501 ttgatagagc aaatattgca gcaaaaagta aaaaggaaaa atatgaacat tcatattcat
     4561 tttttaatga agatacaaga aatcgattgt acaaggaaaa acgattggaa gataatatga
     4621 ataaagcttt agaaaaaggt gaatttatag tatattatca acctaagtat agtttagatg
     4681 atgtaaatga aattgaagga gcagaagcct tgataagatg gaatagtcca gaatttgggt
     4741 ttatttcacc tatagatttt gtacctttat ttgaaaagaa tggttttatt gtaaatattg
     4801 atatgttcgt gtttgaagaa gtatgcaaaa cattaaacaa gtggataaat aaagggtata
     4861 ctcctgtacc aatctctgta aatatgtcta gagttcattt atatagggat aattttatag
     4921 aaaatataac tgatttgata agtaaataca atatatctcc tgagtttatt gagcttgaac
     4981 taacagaaag tgttgtgttt gataatttaa atatattgat tgatataatg aaaaagatta
     5041 aagagatagg attccttata tcaatggatg attttggttc gggatattca tctttaaatc
     5101 tgttaaaaga cctttctttt gatattttaa aattagatag aggattttta atcgaaacaa
     5161 cagatacaaa acgtggaaaa attattattt ctaagattgt ggaaatggca aaagcgattg
     5221 atattaaagt aatctgtgag ggcgtagaaa cttatgaaca agtagagttc ttgaaagaga
     5281 taggttgtga taaggttcaa ggatacttat ttgctaaacc tatggtatta gatgaatttg
     5341 aaaaacactt aaactttaaa tttgattaat cattaataat tattagacta taagataccc
     5401 taacaactta cttacaaata tgtaagttaa ttgttagggt atttttatgg ttaaagtagt
     5461 atattcacga tattatggta ttgtagtaaa aataaaaaat tatggagaat ataaaatgaa
     5521 catattgagt attagaaata tatcaaaaac atattgtgga aatattccat ttaaagcatt
     5581 agataaggtt agtctaaata tagaaaaggg agaatttgtg tctgttatgg gaccatctgg
     5641 aagtggtaaa tctactcttt taaatataat atctacagtt gatagacaga gtgaaggtga
     5701 ggttgtatta gatggatatg atgtaagtaa attgaaagga gaaaaacttg ctgaatttag
     5761 aaggaaacaa cttggttttg tatttcagga ttttaatttg attgacactt taactgttgg
     5821 cgaaaatatt atgttgccac ttacactaga gggggcaagt atcaaagata tgaatataca
     5881 aactaaaagt atatctaaat ttttaggaat agataaaatt ttagatagaa aaacctatga
     5941 aatttcagga ggtcaagctc aaaggtgtgc tattgctaga gctattataa ataaacctgc
     6001 catacttctt gcagatgagc caactggaaa tcttgattca aaatctactg atgatgtttt
     6061 gaaattattt actaggataa acaaagaaca aaaggtaaca acacttatgg taactcatga
     6121 agcatatagt gcaagttatt cagatagagt aatttttata aaagatggtt gtatatatac
     6181 agaaataaaa aaatctgaaa gcagtaattc tttctattct gatatactag ctgttttgtc
     6241 tcaaatagga ggtgtaagat agtggatttt agaaagtttg cttataataa tatagttaga
     6301 aatttcaggg catatttagc atatttttta agttctgttt tttctataat gatatttttt
     6361 gtttttgcag tgtctatgtt ccatccaatt ataacaaaat ctggaatgca gagtggttct
     6421 actgcattta tggctcttat gacttcagaa ttaataatat tcggttttag tctacttttt
     6481 atattgtact ctttaggaaa tttcttaaaa tatagattga aagattttgg agtattgatg
     6541 ataattggaa tgagtaacag acaactgaaa aaattgatat taattgaaaa tttgataata
     6601 ggatttttag cagtgatgtt aggaatatta cttggtttat caatttcaaa attgttttta
     6661 ttgtatttaa gcaaactgtt ttatatgaat ttaactgaat tttattttcc agtaaaagca
     6721 atgctattga ctattgtatc atttatgatt ttatttttga taacaggacc aatgagctta
     6781 aaattattga gtaaaaagag cattttagag cttttagtag gtacaaaaaa gcctaaaaaa
     6841 gagataaaat cttcaaagct atttggaata attggtgtaa tatgtcttat attaggatat
     6901 gctatgatac tatttggcaa taagttaaaa ataaatggag gtatgtatac aacagcacta
     6961 ttaattactt tggggatatt tttatttttt tctcaattta ctattttgat attaaagtat
     7021 ttaaaaaata aagaaaaatt ttataagaac aaagtaaata tgttacttat aagtaatttg
     7081 atatataaat taaaggataa tacacgattg ttatttttaa taacaatact tttatcggct
     7141 actttagtag catttacaac aacttcaaca cttgtaagtt ctcaaggaga agcttctaaa
     7201 aataatttcc caatggttta ctcatatttt tcagagcata ataatgaaaa agaatatgaa
     7261 gaactacaac aaattagaga agcaatcaaa ggatatgatt ataaggagtt aagttttcct
     7321 gtacttaaat ataataggga agtcttacta agtgtaaaga attataacaa attatcaaag
     7381 gaattgggat tagataaaat tagttcaaag ttagaaaaat atgaagggat tataattcct
     7441 agtagcaatt cgcaagaaca tataaattct ttaaaacaat taaaaaatta tagtataaaa
     7501 aatttaaata tagaagtaaa aaaatcaaaa aataaaataa tttttcctac aggactgttt
     7561 agtaaagttg tggttataag tgatgactta tacaatgaag taaaaggtga attttcaaaa
     7621 attaattttc atggatttga ttataaaaac tggcaaagca gtgcaaagat aactgaaaat
     7681 ttaaaagaaa aacattttaa tttggataga gaatataaca ggtcatattt tttaacatta
     7741 ccagatatgt atttagtaga gttacaacaa agtaaaattc ttcaatttat gggtgttttt
     7801 ataggagtga tattatttat agcagttttt agttttttat acttcagatt atatacagac
     7861 gagatagaag ataatgtaaa gtacaaaaaa ctatcaaaaa taggtctatc ttttaaaaat
     7921 atgaataaga ttgttagtat agagataggt acattgtttt ttataccgta ttttatagca
     7981 attataaata gcataatttc tttactgttt ttaaatcaag tattaaataa tagttttaag
     8041 ttagaaaatt tgggtatatt actactgata tcatctatat attcacttta tttttactta
     8101 ttaaaaaaaa attatactag aaaagtttgg ttttttgatt aaagtggtat ataatagata
     8161 tataaattaa tatttaaaaa gtagttaata ttaggggagg gcatttgcac ctcccttgta
     8221 atatgtggaa aattgaatat tagaagaagg aggagacttg atggaaaatc aacaattatt
     8281 aggaacagaa cgaattagta aattactact aaaatattct ataccagcta taataggtat
     8341 gttggtaaat agtttatata atgtagtaga tagaattttt attggaaata taccaggggt
     8401 tggaccactt gcaataacag gtcttggtgt aacaatgcca ataatgacaa taatacttgc
     8461 atttgggatg ttgataggta taggaactac tactaccata tcaataaaac ttgggcaagg
     8521 taaagtagaa gaggcaagaa aacttatagg aaatgcaatg acgttatctg taataacagg
     8581 aattatcata atgatacttg gaatattatt tgcaaataaa atacttacat tgtttggagc
     8641 aagtgaaaat actttaatat atgcaaaatc atatatcaat attatattat taggtacagt
     8701 agtaaatctt ctttcctttt ctttaaacca ctcaataaga gctgatggta gtcctaaaat
     8761 ttcagcagga ataatgatag taggatgttt aactaatata gttttagact ggatattaat
     8821 atttggattc aatttgggaa tacaaggagc tgcaattgct acagtaactt ctcaggctct
     8881 tacagctatt ttaactatag ggtattatat aagtggaaaa tcaaacttac gattcagtaa
     8941 atctaatcta aaattagata aaaagctaat aaaagcagtg tttgcaatag gtatgtcgcc
     9001 atttgctatg caacttgcag caagtttagt tcaagtaata tcaaatattg cacttaaaac
     9061 tcatggtgga gatttagcta taggggctat ggctactatt tcttctattg caatggtatt
     9121 tttgatgcct atctttggaa taaatcaagg agcgcaacct attataggtt tcaattatgg
     9181 agctgaaaaa tatgataggg ttaaaaaagc atatttaggt tcattagtag tagctactat
     9241 aattttatgt atgggtatgg tagtagttat gcttttccca gaagctatta taggtatatt
     9301 taataaagac cctgaactta tgaatatatc tgttaatgga ttaagaatat acttacttat
     9361 gttgccaatt gtaggcttat cagttacagg aacaaacttt atacaatcta taggaaaagc
     9421 aaaaatggct atgttattga gccttttaag acaagtaatt ttattgatac cagcagtatt
     9481 aattcttcct acattcttag gtttacaggg tgtatggaca gctcaaccag tgtctgattt
     9541 tatagctaca gtaattacag gtatagttgt atttagagaa ttaaaaagat atactcctaa
     9601 aactgaaaaa ttaaatgaaa atgaaagact aaacgagata actactgagt aaaaatataa
     9661 attcagataa ttgagcaaga aattgcaaaa aatacgatta tatgataaaa taaaaccgct
     9721 gtcaaggata agagaaattc taattatgga gtgattttat ggaatttaca aagaaaagca
     9781 aattaaaaaa tatgtatgta aacatgaatg tagctagaat gttgtctaag ataaatgagt
     9841 ataagggtag acaactgctt tacaaaaaac aaccaaaaga aattttagaa aacttagaaa
     9901 aaaaatcttt agtagattgt tctgaatcta catacattgg aaatcaaaga gatagtagta
     9961 attttaatct agaaaagttg ataagtaatg aagttacacc tagaagtaga gaggaattga
    10021 gtattgttga atacagagat gtggtaaaaa ctattaacag tgcttatgag agtattccca
    10081 tcagttcaca aacaattctt gagcttcatg gatacttata taaattttca tctactagag
    10141 gaggaagtta taagtcagat aatgatttta ttgaacacaa tttaaaacct agcgaattta
    10201 taagtactga ttcaagtgtc aataaagttg aaaaagctgt agaagaaata tgtgaagctt
    10261 ataatacatt gatagaggaa gatgaaattg atatattaat attaatttct gcatttgtgt
    10321 tagatttcat attgatacat ccattcaaag aaggtaatat aaaaatggcg agagtactta
    10381 tcctattgtt actaaataaa aatgggtatg aagttggaag atatataagt ttaggaaaga
    10441 tatttgatga tagttcatat gaatattata gtaatttaaa ttatttgaaa gcctcaatag
    10501 gtagtgaaaa agcagatatg aatgcatgga ttgagtattt tttagagacg atattaactg
    10561 catatgagaa gctggatgat agtttaaata tatctgataa gaaaagacaa acaaaaacta
    10621 gtagaataga gaaaataata aattctaccc ttggatattt tacaaaagaa gatataagag
    10681 atttatgtcc tgacattcca gaacctacta taaatagagt ttttaataat ctaagaaaac
    10741 aagataagat agaagttgtt gcaagaggaa gaagtgcaaa atggaagaaa aaatattaaa
    10801 aaatgtttag ataactaaaa tggtatagtg ttttacacta taccattttt aatgtcatta
    10861 gcatgagcaa taatttaaca acttaagcaa tatcaatttt atttattgat tatttttgaa
    10921 ctgaaaaatg gcgttatatg gtatacctca gtggtaatta attttggatg agaagatata
    10981 atattacaga atatattagg agtatatgta gaaaaaatat aataaaagtg tgaggcttag
    11041 taattaacaa attatgtggc ttcacacttt tttataaaat aaagatactt agcagaataa
    11101 tattttagtt gatatgttta taagatggta cttgtttggg taaaagtata aatagtaaaa
    11161 aagtagaagt aatgtgtata aaaacataag ttacattctg taataatagt tattttataa
    11221 ttcaacaaaa gaatagaatg ggtatatttg tgaaataata atataaaata atcttaggag
    11281 tgagcttatg aagaaaaaat tattatatat aaatgtaaat tcaaaacctg aagacttatc
    11341 ttcaagtaaa actgtagcaa gaaaatttat aaataaattt atggaaaaaa ataaagattt
    11401 tgaggttgaa gagatagacc tttataaaga acatatacca agacttgagt atcagtactt
    11461 tgaaaaaaga aatagcatag ttagtgaaga aaatgctaaa aatttagacg ataaggatag
    11521 aaaagagctt acaaagatta gaaatctttg tgaccaattt gtgaatgcaa gtgtttatgt
    11581 aatagcagca cctatgtgga gtttatcttt tccagcacca cttaaagagt atatagattg
    11641 tataattcaa gatggaaaaa ctatatcttt tgaaggcaat gataaaccac aaggaatttt
    11701 aaatgatatt gatagaagta tggtatatat acaatcttct ggtggacaca taccatgggt
    11761 gttgaagcca gttatgaata aagggttaaa ttatgtggaa agtattatga aatttatagg
    11821 aattaaaaaa tttgatgaat tgttagtaga tggaactgga actagtgaag aagaaagaca
    11881 agctgcaata gaaaaagcat ctgaaaaaat agatggtata atagatggga tgaaatttta
    11941 attataatat tttattaaaa atataaagga aaaacctctt ttaatgtaga aacttatatt
    12001 gaaggaggcg agaatcatgc gagaagaaaa aagtaatgaa aagtatgatt gttattggtg
    12061 taatcaagag aataatttct gtgtagaaat aaaagataat atagtcatga tagatgatgg
    12121 cactggtacg ttaaaacaag ccgttttcat agggtataaa caaatccaaa ttaatttaaa
    12181 ctgttcacat tgtcaaaact taaatagaat aaaattaaat ttgtagaatt ttaaaatgga
    12241 aattattgaa ggaattaaaa atagaaagtg ttaatattta gactgaaaat tatattttat
    12301 acaataaaag tttatttaaa taacattgta ttttaatatt tattttcagt cttactttat
    12361 taaaattatt ttttggttat gtctggaaaa tatctgtcta ctaatttttc tgaaatattt
    12421 tttaattggt cagccattga agtcagactt aataaggcta tcaataaatc atctttattt
    12481 tctggattta tactaatatt ttcaaaatta gactctataa agtccggtat attctttctt
    12541 tgattttctt ttattaacat gaatttttcg taacatgaaa ctaaatcttt ttcactgaaa
    12601 ttatcctttt catacacacc atgtaagatt cttttaattt cttgtattgt aagtgtattt
    12661 ttcatagaat aaataattat catctgtaaa atttgttctt tagtatactt ttttccctta
    12721 actggtttga ttaatccctc tttgctgtaa ttatttatca tggttttagt aagaatttta
    12781 tcactttcaa atcttttatt agcagaaagc ttattgtcaa ttagagaaat aatttgatcc
    12841 atatataagt caatagatgg aatatcgttt gaagttatat ctgcattatt tagtgtttct
    12901 agtattatag tattaagttc attattcatc atatgttacc tccataatat atataataca
    12961 ctaaattaga aagtaaaaca atattaaggc ttgtaaaaaa aacataaagt ggtataatta
    13021 aatatataat atagtaatca aaactatatt tatgaatctg atttttaaaa tacataattg
    13081 taggaggacg aaatgtatca atttttttta aagggtagag acccaataag cagtttaact
    13141 cattttatag gtgcttgttt atctctttta gcaactataa ttttagtttt tcaatctgtt
    13201 acattacagg aaacttctct cttgatgata gtatctgtat ctgtatttgg actttcacta
    13261 attgctttat atagtgcaag ttcgtattat cattttttaa aggggacacc agaacaagaa
    13321 ttgtttttta gaaaagtaga ccatgcaatg atttacgtat tgattgctgg ctcttataca
    13381 ccaatatgtc tgaattttat ggaaaaaaaa gaaggcataa tatttgttac agcaatatgg
    13441 atagttgcat ttattggcat aataataaaa atattttgga tggatgcacc tagatggtta
    13501 tctaccagta tatatttact tatggggtgg gcaattgttt ttgatataaa tgcattcaat
    13561 tcaattccta aggattgcct tagactatta attatggaag gtgtatctta ctctataggt
    13621 gcgattattt atataattaa aaagcctaat ataagtcctg agtttggatt tcatgaaata
    13681 tttcatattt ttattatgat aggttcttta tttcactttt tagcagtttt attgtatgtt
    13741 ttatagtttt aaaagatttg attaattgat actaaaattc tacaatgaat aaaatggaac
    13801 tgccttatat agattatatc ttagacaata agacagttcc ttattataaa tattaaattc
    13861 tacttataat aaaattttga taaaaatagt ccattcaaaa tgcctattcc ataattcctt
    13921 taatgtactt ttcatttacg acatatagtt tgaatttatg tactataatt atatcctcta
    13981 aattctctga aacactaaca attctgacac atttgtttct aatttctaca ggtaaatcta
    14041 aatcatttgt taaattcaca tagtcatcat atttatacat aaaatcatcc ctttacatta
    14101 agatatactt attatactat attttaataa gtttttttaa attattacaa tttttcccga
    14161 aatttcattt tttatacata aaatttcc
//
LOCUS       DAMJVI010000091        14158 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.096, whole genome shotgun sequence.
ACCESSION   DAMJVI010000091 DAMJVI010000000
VERSION     DAMJVI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.096"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            122..1750
                     /gene="aspD"
                     /locus_tag="PC545_002849"
     CDS             122..1750
                     /gene="aspD"
                     /locus_tag="PC545_002849"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HDF2457609.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAITETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFVHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKTASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            1871..2866
                     /locus_tag="PC545_002850"
     CDS             1871..2866
                     /locus_tag="PC545_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDF2457610.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTDNLDEIKDFFEKGIYC"
     gene            complement(3055..3438)
                     /locus_tag="PC545_002851"
                     /pseudo
     CDS             complement(3055..3438)
                     /locus_tag="PC545_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      3664..3761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     repeat_region   3903..4260
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3903..3931
                     /rpt_unit_seq="atttacattccatatagttaatataaaac"
     gene            complement(4546..4704)
                     /locus_tag="PC545_002852"
     CDS             complement(4546..4704)
                     /locus_tag="PC545_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457611.1"
                     /translation="MDNFLQGILASLSASLIVYIASKLFRKRKKPLKAATKSGWEFDL
                     KIRFHKTK"
     gene            complement(5063..6433)
                     /locus_tag="PC545_002853"
     CDS             complement(5063..6433)
                     /locus_tag="PC545_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp29"
                     /protein_id="HDF2457612.1"
                     /translation="MKLKKLLSLGLAVSMFAAGPISANALDTIYTIQGKDKYETAALI
                     ADEQNYTTAILINADSTMADGLSASGLAGATNAPIFLTKKNNIPNATLKRVEKAKKVY
                     IIGGESSIDKATETLLKDKGIEAKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAFK
                     GEPDAMSAAPVAVRDKAAIVLTDGKSVPFNTTGVESYVIGGTSSMSDKLVNDTNSTRL
                     GGVDRYDTNKKIVNKFYNGAKEFYIASGTDLVYALVASPMAKNNPIVLVDIGSNKDIL
                     KNAAKVTGIGNLSDKIMLECENTINNISLPSSNKNIICGLKIDFDSKHIIWQDKGSYL
                     ECYDDNLEYYTEKYKDPTVPINIENTSTQNLDVTKLKVYGEANSTLYMGQYAKIKMDF
                     NSFGVLKPGEKCTIYLDIISLDPSRSMTLTFSYVGDKENYKYEQGLASRILFYNPDGE
                     DFFEYK"
     gene            6716..6814
                     /locus_tag="PC545_002854"
                     /pseudo
     CDS             6716..6814
                     /locus_tag="PC545_002854"
                     /inference="COORDINATES: protein motif:HMM:NF026008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            6833..7254
                     /locus_tag="PC545_002855"
                     /pseudo
     CDS             6833..7254
                     /locus_tag="PC545_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7485..7757
                     /locus_tag="PC545_002856"
     CDS             7485..7757
                     /locus_tag="PC545_002856"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2457613.1"
                     /translation="MSKHQFKSVSTFFTKKNVSQFYKKNNLKKIRIHDLRYTSATLLL
                     LGGTNMKVVSERLGHTDIKITMNRYSHVLKEMDKEASDNLSELLFK"
     gene            complement(8075..8743)
                     /locus_tag="PC545_002857"
     CDS             complement(8075..8743)
                     /locus_tag="PC545_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2457614.1"
                     /translation="METVTNNQSGVEIQLSLFEAIKDLLLINPIFLLGIILIGIFLVV
                     IFKKNSDIPKVKTSILSLAMYYYLCLTLSHIVGIPTFGEYIRLSQLGETFFNPNINLI
                     PFSDGFSLSFILNILLFVPLGFLCPLISKTFERLRNTFLIGFGLSLFIETIQLFTLHR
                     ATDIDDLLTNVIGTIIGYFCFKLIAKLRIVKLHSDDDSMEQDYTVCIPIAIIIVALIL
                     GFFS"
     gene            complement(9579..11999)
                     /locus_tag="PC545_002858"
     CDS             complement(9579..11999)
                     /locus_tag="PC545_002858"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDF2457615.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            complement(12353..12703)
                     /gene="rsfS"
                     /locus_tag="PC545_002859"
     CDS             complement(12353..12703)
                     /gene="rsfS"
                     /locus_tag="PC545_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDF2457616.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            complement(12820..13392)
                     /gene="yqeK"
                     /locus_tag="PC545_002860"
     CDS             complement(12820..13392)
                     /gene="yqeK"
                     /locus_tag="PC545_002860"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDF2457617.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDTLRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            complement(13393..14082)
                     /locus_tag="PC545_002861"
     CDS             complement(13393..14082)
                     /locus_tag="PC545_002861"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDF2457618.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
BASE COUNT         4771 a         2297 c         1801 g         5289 t
ORIGIN      
        1 ttattttaat ttcatacttt actttttcta taaacctatt ataattaaag tataatcatt
       61 tttgcaactt gtttatttta ttataaaaat gtgaaactta ttacataagg aggaatttaa
      121 catgaaaaat catacttata aagaaatcaa aaacatatat ggaaaaataa gcccttttga
      181 atttaaggac aaattaatag atattgcaaa atacactgca aaagaaaata acagagaatt
      241 acttgatgca ggtcgtggaa atccaaactg gacttgttca actgctagag aagccttttt
      301 tacttttggt catttcgcta ttactgaaac tcgttctaat tgggatttag gtcatttggc
      361 tggaatgcct caaaaaaagg gtattaaaga aagatttttt aaatttataa atgaaaatat
      421 agatatgcct ggagcatatt tagctagaga tattataaac tttggtataa atgaacttgg
      481 atttgatgga gatgaatttg ttcatgaact agctgatgga attattggtg ataactatcc
      541 acttccagat agaatgttac ctcatatgga aaaaatagta cacgattacc tagttcaaga
      601 aatgaaatat gatatatcag gtaaatatgg tgatgtagaa atttttgctg ttgaaggtgc
      661 tacagctgct atgtgctata tatttgattc attaatggca aatgaacttc taaaaaaagg
      721 agataccata gccttaatga ctccaatatt tacaccttat cttgaaatac ctaaccttcc
      781 tcgttacgac tttaaagtgg taaatataaa tgctaatgaa gttgatgaaa aaggtgctca
      841 tacatggcaa tatacaaaaa aagaattaga gaaacttcgt gacaaatcta ttaaagcttt
      901 attcgttgtt aatcctaata atcctgcttc tatagctatg gatgaaacat cttgtaataa
      961 tctaatagat gtaattgaaa attataataa agatttaatg ataatatcag atgatgtata
     1021 tggaactttt gtagaagatt tttcatcctt aatgtcaaaa ctaccttaca acactgttgg
     1081 agtatattct tattcaaaat attttggagt tacaggatgg agacttggta catttgccct
     1141 tcataagaaa aatgtttttg ataagaaaat aaatgattta actggagaat taaaaaaatc
     1201 agtagataag cgttacagtg atatgagtct taacccttct agtctttcct ttatggaaag
     1261 agttgttgct gatagcagac ttgttgctct caaccatact gctggtcttt ctactccaca
     1321 gcaagtacaa atggcgtttt tctctgcttt tgcattgatt gacaaagtag atgcctacaa
     1381 aaaacttaat atgagtatct gccatagacg tcaaaaacta ttatttgaag ctttagagct
     1441 tccaattaat gaaaacaaaa acaatgctgc atattacact caatttgata ttgaagaatg
     1501 ggctaaatta aactacggag aagacttatt taaatttata agtaaaactg catctccagt
     1561 agatgttctt tataaattag ctaatgacta ttcagtagtt ttacttagtg gaaatggatt
     1621 ctatggccct gaatggtcaa ttagaatatc tctagcaaat ctatatgatg aagcttatac
     1681 taagatagga aaagccataa gagagatatt aaatggttat atcgttgaat ggcaaaaaat
     1741 taagaaataa ttatattaca ctgaaatatc taaattatat gaataaaaga gtttatgtag
     1801 accttaacta ctttttttga tataatccta tcggaattat aaattttatt tatgaaaggc
     1861 agagaatagt atgagtatta agaaaaaagt tgccatagtt tttactggtg gaactatatc
     1921 tatgacagta gatgataaag tcggagcagc tataccaact ttatcaggtg aacaaatact
     1981 atcaatggtt accaacattg ataaagttgc agatgttgaa gtttttaatt ttgatgaaat
     2041 tccaggacct cacataacac cttatagaat gatggaattg aaaaattatg taaatgattt
     2101 attagcaaga aaagatatta caggtgttgt aataacacat ggtacagata gtttagagga
     2161 aacagcttat tttctagacc ttacaattga caacatcaaa cctgtaatag tcacaggtgc
     2221 tatgagaagt agttctgaac ttggatatga tggttcaagt aatttatctg catcagtttg
     2281 tactgcagta tccaaagatg ctatgggaaa aggagtcctt gtagttttaa acaatgaagt
     2341 attattagct tctgaagcta ctaaaacaaa tactctttct ctaaatacat ttaaatctct
     2401 aactagtggc ccattaggaa taattgactg caacgaactt gtccttacaa gagatattgt
     2461 aaatagaaca attatagata ctgataaggt agaatctaaa gttgccttat ttaaagcata
     2521 tgtaggagaa aatgctgact ttataagatt tgctgttgat agtggttata aaggtatagt
     2581 tattgaggca atgggcagag gtaatattcc tcctcaaatg ctttctggag ttgaatatgc
     2641 tagagaaaaa ggacttcctg tagttatagt ttctagatgt cattcaggta gagtattcga
     2701 tagttatggt tactttggtt ctggtagaga tttaaaaaat cttggttgta tatttggtgg
     2761 ggacttacca ggtcaaaaag ctagaataaa acttatctta gcacttggaa aaactgataa
     2821 ccttgatgaa atcaaagact tctttgaaaa aggaatctat tgttaattct aataacttct
     2881 atttaattat ttattgatat ttcaacattt ttaaatcaaa atctaatact tatgtcagtt
     2941 aaatgtcagc aaaatatttt atccacaatt tttacacaaa ttttccacaa cttatcccca
     3001 atattatcca caagaaaaaa ggtagcactt atattagtag ctacctttta tttattaatc
     3061 ttcatctttc cagcttttta tccaatttcc tatttctatt attttttctt cagttatttc
     3121 ttctccatca tgtaattttg aaattagttc tgataatgga ttgttatgta gacctccaaa
     3181 aattcttttt ccctctattt tgagatattc tttctctttt attaatactc tatagtgtac
     3241 atgtttccca attctctgcg atatgaaaaa acctttttta gttaatctag ctaaaagtgt
     3301 aattgtagtt gtatgcttcc aagcatatgt tttttctatt taatctgcaa catcttttga
     3361 tattacagtt ttataaccag tattccatat gtatttcata atttttaatt ctgcatctgg
     3421 taatttttta ttcaacataa tatattcctt ctttcaattt atgtcacttc ctcaaactta
     3481 tctagcttaa attaataata ggagcttttt atcacaaaaa ctacaggtaa atttttctat
     3541 atattctacg tatttagtct gtttttaagt gtgaaattac gttcatacct ataaatttat
     3601 tcaattttca aacaatcttg ttgaataata actaattcta ttgtattatt tagttataga
     3661 ataaaactaa atcggcaaaa ctagagaaat ctagtgacgc aaagctatag ggactaagac
     3721 ttatataaat atcttatgag ttatgttagc cagttgccaa agagatattg ttctttttgt
     3781 ttttatgaaa gttttttagg gggataataa tttatatgtt tagagataaa atggataaat
     3841 gtacacatat attgactgct tatattagca attcgtatga ttattgtaat tttttggata
     3901 caatttacat tccatatagt taatataaaa ctcaattaaa ttatgacctt ggtttagata
     3961 gttcattatt tacattccat atagttaata taaaaccaga aggaatatct ggagttattg
     4021 gagggtctat tggatttaca ttccatatag ttaatataaa acatttacta agtgtagata
     4081 taattataaa tacgatttaa tttacattcc atatagttaa tataaaacct gtagacttca
     4141 accctacaat gcttgttgga agtgtattta cattccatat agttaatata aaacttttgt
     4201 aaaagtttag aagtttatat ataaaaaaaa tatttacatt ccatatagtt aatataaaac
     4261 cccaaaataa attgagtatt tccaatgcct acacatatac aaatctctca aatttgcagt
     4321 gaaccatgag tagtgcaatt gataacactt atcacaagtc ctcaatgcct tatatttcaa
     4381 ctgttaagcc ttaatttctt caaaaaatcg aacactgcaa aatttctatg actttattat
     4441 atcataaaaa tattattttt gaatatctgt accaatttgt gatataataa aagcaagaag
     4501 aactacaatc tattttgctg tagagtggag ttcataattt agaaattact tagttttatg
     4561 gaatctgatt tttaaatcaa attcccaacc actcttagtt gccgctttga gtggtttttt
     4621 acgctttcta aatagtttgc tagctatata aactattaag ctagcagata gacttgctag
     4681 tatgccttgt aaaaaattat ccatgtatat tcacctccct tcttagcgtc gggaggataa
     4741 ttttttgata catgaactcc actctataaa ttgtagatta catcttcttg ctaaaaatat
     4801 tataacatat aattcttaca tattttacct atttattact ttttacatta taataatatt
     4861 cttttaatca aaaaaacacc tactattaaa gtaaatgttt ttaagtattt ttaattttaa
     4921 aatccatata gttcatatga aactaaatat aataactcaa catatgagca atatcgagac
     4981 atttacatac cacatagtta atataaaacg aaaataaaaa aattaaactt ataaaaccct
     5041 tattaattta catacccact agttatttat attcaaaaaa atcttctcca tctgggttat
     5101 aaaataatat tctacttgcc aatccttgtt catatttata attttcttta tcacctacat
     5161 aagaaaatgt taatgtcatg cttcttgatg gatctaatga aattatatct aaataaatag
     5221 tacatttttc tcctggcttt aatacaccga aactattaaa atccattttt attttagcat
     5281 attgccccat atataatgtt gaattagctt ctccataaac ttttaatttt gttacatcta
     5341 aattttgtgt acttgtattt tctatattta taggaactgt tgggtcttta tatttttcag
     5401 tataatattc aaggttatca tcataacatt caagataact tcctttatct tgccaaatta
     5461 tgtgctttga atcaaaatca atctttaatc cacatattat atttttattt gaagacggaa
     5521 gactaatatt attgatggta ttttcacact ctagcataat tttatcactt agatttccta
     5581 tcccagtcac ttttgcagca tttttaagta tatctttatt acttccaata tcaactagta
     5641 caataggatt atttttagcc attggtgaag caactaaagc ataaactaaa tcagtaccac
     5701 tagctatata aaattctttt gcgccattat agaacttatt tactattttt ttattagtat
     5761 catatctatc tactccacct aatctagttg aattagtatc attaactagt ttgtcactca
     5821 ttgatgaagt accacctatt acataacttt ctacaccagt tgtattaaat ggaacacttt
     5881 ttccatcagt taatactata gctgctttat ctctaactgc tacaggtgca gcactcattg
     5941 catcaggctc tcctttaaaa gcattagtca atataacttt atttactttg ttaattgagt
     6001 ttatttcttt tgcaacatta tagcttgtct taattctatc actaccttga agtcttttag
     6061 cctctattcc tttatctttt agaagagttt ctgttgcttt atcaatagaa ctttctccac
     6121 ctattatgta taccttttta gctttctcta ctcttttaag agttgcatta ggtatgttat
     6181 ttttcttagt taaaaaaatt ggtgcatttg ttgctcctgc aagtccactc gcacttaatc
     6241 catcagccat tgtgctgtct gcattaatta atatagctgt agtataattt tgttcatcag
     6301 ctataagagc agccgtttca tatttatctt ttccttgaat agtataaatt gtatcaagtg
     6361 cattagctga tataggacca gcagcaaaca ttgacacggc taaccctagt gataataatt
     6421 tctttaattt cacaatatct ccccctttta tatatgaaat actatatatc ttacataatt
     6481 ctacattaaa ttttcttttc ctcctttaac gatatttatc ttcctgttaa tttaagtgta
     6541 tacaaatacg tgttataatg taactgtaaa gaattaaaaa gataatgaca taattagagc
     6601 agtccaactg ttcttttata aaactaactt ataaagggag cgtacatata tgaatatcaa
     6661 atcagctttt ataagaaaaa gatgggaaaa attctatgta tatgtggaat aagaaactgg
     6721 caaaaagaaa caaaaaagtt atggaagcta taaaaagaaa aaggatgctg aaaaacattt
     6781 aattgaaata aagtctacta taaataataa taagtttgtt gctccaaaag gtatgacatt
     6841 tgtagaaaga tgttataaat atatagacat gaataaaaat aattggtctc catatactgt
     6901 tacaaataca aaatcttggg ttaaaaatta catagaacct tttttaagaa tctaaaactc
     6961 acagaagtaa atcctagttc agtacaagct tttgtaaatt ataactttaa taattctacc
     7021 tttactagtg ctaaagtaag gtatagcttt ctaagttctg taataaggga agcgtataga
     7081 ttaagagaaa tacaagaaaa ttcatgtgat tttataaaac ttccttctaa agaaaaatat
     7141 tctacaaatg aaatatacaa tagagaagaa gtattattgc taatagaaaa gttacttggt
     7201 aataatatag aaatacctat tcttttaatg ttacttctag gtttaagaat gtgagaagct
     7261 attgggctta ggtggtcaga tgttgactta gataacaatc tgataaatgt taatcaaatt
     7321 cttgtctatt ctaatggcaa aatatctttt aaagagccaa aaacaccaaa atcaaaaaga
     7381 gctttatcag ctccaaaaga attaattgaa aaattgaaaa ttgaaaaact aagacaaagc
     7441 aaattaaaat tgtaaggtat cctggaaaac agaaataact tagtttgtct aaacaccaat
     7501 ttaaatccgt ttctaccttc ttcactaaga aaaacgtttc acaattttac aaaaaaaata
     7561 atttaaaaaa gattagaata catgatttaa gatatacaag tgccactctg ctgcttttag
     7621 ggggaactaa tatgaaagtt gtttctgaaa gattgggtca tacagatata aaaataacta
     7681 tgaatagata ttctcatgta cttaaagaaa tggacaaaga agcttccgac aatctaagcg
     7741 aacttctatt taaatagtaa aaactgactg tcagttaaat gtcagttaaa cctaaaaaaa
     7801 taggttttgt cagttgatta tcagtcaatt attttataat agtttcactt aaaaacgtct
     7861 atatctacac attttactat ttgtttatgc ttattaatat ctatctgtta ttattaattc
     7921 taatagcttc tatttaatta tttagttcag tttacaggaa actacacaca aaccataaaa
     7981 taggatgttg acaatataac agatagtatg ctcctgtaaa aggtaacata gtatctgtta
     8041 tattgtctgg attcccaaat tagctatttg gaacttaact aaaaaatcct aatataaggg
     8101 caactattat aatggcaatc ggaatacata ctgtgtaatc ctgttccata gagtcatcat
     8161 ctgaatgtag ttttacaatt cgcaatttag caatcagttt gaagcagaaa tagcctatta
     8221 ttgttccaat tacgttcgtg agtaagtcgt ctatatcggt tgctctgtgt aacgtaaaaa
     8281 gctgaatagt ttcgataaaa agagataatc caaaaccaat caagaatgta tttctcaaac
     8341 gttcaaaggt tttgcttata agcggacata aaaaacctaa tgggacaaaa agtaaaatat
     8401 tcaaaataaa actcaaacta aatccatcac taaaaggaat aagatttatg tttggattaa
     8461 agaacgtttc ccctaactgt gacagtctta tatattcacc gaatgtaggt ataccaacaa
     8521 tatgcgatag tgttaaacat aagtagtaat acattgccag tgataaaata gatgttttga
     8581 cctttggaat atcactattt ttcttgaata ttacaactaa gaaaatacca atcaagataa
     8641 ttcccaacaa gaaaatagga ttaattaaca ataaatcttt gatagcttca aacagactga
     8701 gttgaatttc tacacctgat tgattatttg ttacagtctc catttgtaca tacctcctca
     8761 taacatttca attatttcta aaaaacaaaa tagaagataa atcttaaaat catcctatca
     8821 aaatttttaa atttgccttt atcagtttat accaccagtt aattcctgtc ttacttctgt
     8881 atggtattct tgggattctt tcatcaaatt agacaaaaaa tggttaccat atcaataaac
     8941 tggttacgct ccatatctcc cgaagcaacc atcccctgag aataaatccg tatcaggaat
     9001 ctccacatct actgcaccta tcacaagtta tttgataaca tcctcagaca atcccaactt
     9061 ggatatatcg acaacaccca ttagaatacc actgctgacc atacttgttt tcctgctgat
     9121 aattttaatc aactgggaag ttgaaacgct atctttttta caagttcctt tttagaaaca
     9181 tggtagctat tatatgaatc tgaatttcgt tgtctgggtg tttcatataa agcatagata
     9241 tgcacctttt ttgcctgttt tcattatata gaaaaaaatt tcaaaataca ataattatat
     9301 ttttttcgtt taagcttaga gcagttcctt gacgaacaac actttggaaa ctgaatgatt
     9361 tacataccac ttaatacatt aactcgattt aacattaatt ataataatat ttaaaacaac
     9421 aactagggta tataagaatg aactttttag ttctttaatc atttttttgt ataaaatcaa
     9481 atctattttt ataatttcaa caatgaaaaa aggcttatct ctattttgag acagcctcat
     9541 ttattttata aatttatatt tttgttcata tttttgagtt atttaacaac tatatttact
     9601 aattttttag gtacagctac aactttaact atagttttac cttcaactaa tccttttatt
     9661 ttttcatctt ctaaagctac cttttccatc tcatctttag atatatttga gttaacagtt
     9721 aattttcctc taacttttcc attaacttgt acaacaactt ctatttcatc ttttactaaa
     9781 gctttttcgt catatttagg ccagtctata tcaaatacac ttccttcttt acctatcata
     9841 gtccatagtt cttcacctaa atgaggggca aatggtgcaa ttatagttac tatagtttgt
     9901 acaccttctt ttataactgc ttcgtttata ttatctaact ctttatattt atacatatca
     9961 tttattaatt ccataagagc tgatatagca gtattaaatc cgaatttttc acttaaatca
    10021 gcagtaactt ttttaagtgt agagtgtata gtatatctca tatccttatc ttgggaattt
    10081 aattctccaa actctacatc ctttttaacc acatctgcta actcgtcaac caatctataa
    10141 accctattta agaatctaaa gcatccatca acaccttgct ctgaccagtc caaatctctt
    10201 tctggtggtg ctgcaaataa tacgaaaagt cttgcagtat ctgctccata ctcatctatt
    10261 atatccatag gagatactgt attcccttta gacttactca ttttagaacc atccataagt
    10321 accattcctt gagtaagtaa gtttttaaat ggctcattga agtctaccat tcccatactc
    10381 ttaaatgcct ttacaaacca tcttgcataa agtaaatgca ttatagcatg ttctactcca
    10441 cctatatatt ggtctactgg atgccatcta ttaactaatt ccttactaaa tggttcattt
    10501 tcattattgc tatccacata tcttaagaaa taccaagaag aatcaacaaa tgtatccata
    10561 gtatcaactt cccttctagc tggttttcca cagtgaggac aagttgttga cataaattgc
    10621 tttgaagtcg taagtggaga ttcccctttt ccagtaaatt caacatctgt tggtaaaaga
    10681 actggtaaat cttccttttt aactggaact attccacatt catcacaata aaccacaggt
    10741 ataggacaac cccagtatct ttgtcttgaa actaaccagt ctctaagtct ataattaatt
    10801 gttctctttc ctctaccttc tttttctaac ttatcgatta ttccttcaaa tccttctgaa
    10861 gcatctaatc cattaaattc ttctgaattt atcattttgt tatcttctgt tattacatca
    10921 attatgtcta aattatattt ttcagcaaaa tctctatctc tttcatcatg tgctggaact
    10981 gccattatag cacccgttcc atactcaact aatacatagt tagctatcca tagaggaact
    11041 ttcttgccat ttaaagggtt tataacatat ctacctataa acattccttc tttttctaca
    11101 tcagctgctg ttctttctat ttctgtcata gtatgcattt tttgaacaaa agcttcaaca
    11161 tctgcttcat attcagtccc tgcaaccaat tctttaacta attcatgctc tggagcaagt
    11221 accatataag taactccata agttgtgtct ggtctagttg taaatactgt catagattta
    11281 tcagttccat ctatatcaaa aactaaatct gctcctgtac ttttacctat ccaatttttt
    11341 tgcattgttt taactttttc tggccatcca tctaatgtat ctaagtcatt aagtaattct
    11401 tctgcaaact cagtagtctt aaaataccat tgttctaaat ctttctttaa tactgttgct
    11461 ttacatctct cacatgctcc ttgaacaact tgctcatttg caagaacagt ctcacaagaa
    11521 ggacaccagt taacatatga ttttttctta tatgcaagcc catgctctaa aaacttcaaa
    11581 aatatttctt gtgtaaatct atagtattct ggagttgaag ttgcaacttc tttatcccaa
    11641 tcataactaa tccctaataa atttaattgc tctttcattt cttctatatt ttccattgtc
    11701 catttatgcg gatgtattcc atgttttata gctgcatttt cagctggtaa tccaaaagaa
    11761 tcccatccca ttggatgaag tacattatat ccttccattt ttttgaatct tgcgacaaca
    11821 tcacctatag aataatttct aacatgtccc atgtgtattt ttccagatgg ataagggaac
    11881 atttctaatg tgtaataatt aggtttttca gtttgtgcag tatccatttt atactgatta
    11941 ttatctttcc agattttttg ccatttcgat tctacttctt taaaattata tacgctcata
    12001 ttttgcctcc tatttttaat tgtgtataac taccttattt agtattattt acttataatt
    12061 tccaaataat tctttgaaaa taaaaaatcc tcgtcactaa acttacgttt agggacgagg
    12121 ataaatcttc gcggtaccac cctaattagc ctactacata tatagactct cttagatagc
    12181 ttttaatcca gccatggaga atagatttta atctagtaag ttcaaagtta tataatatta
    12241 gttctcacct tccactaact ctctgaaaaa atataatctt ttactgctct agtctattca
    12301 atttaatata atttttgaaa ttttctaatt ataataacat atattatcat gtttatgcta
    12361 atgtgtcaat atctatataa ggtgcatctt tccataattt ttctaaatta taaaaccctc
    12421 tattttcctc attaaataca tgaaccatta tgtctccata atcaaggagt acccaagttt
    12481 gagttccttg tccttcttta cctcttggct ctaatcctag ttttgctaat tcatcttcca
    12541 cattgtcagc aatagctttt acctgtctct gagaagatgc tgtagtaata ataaaataat
    12601 cacacaaact agaaactttt cctatattta ttataactgt atcttgacct agtttatctt
    12661 ctatggcatc atatgctatt tttgtcattt gttccactgt catataaaaa ttcctcactt
    12721 tcctaattaa aatacactta gttattgtaa catattaata cgattttgta acatagtcaa
    12781 atttaaattt attaatatgt ttgcctttat atagcttttt tataataact tttctttaat
    12841 tagatagttt cttgccataa ctgttcttgg atgtatctct tcttttttat tgatgacaaa
    12901 cattaaagta ttattaaaag aagttaaaag tgcttcatca agcttccctc catatgctaa
    12961 ttctcttaat gtatctacaa ctgggaattt tcttccttct tcaattaaat cagctatgta
    13021 aattattttt tctaaaagag acatgtcctc tctccctgtt gtatgatatt tgattgcatt
    13081 tataatatct tcatcttcta cttcaaattc atactcgcat atgtacgccc caattacgct
    13141 atgagacaag gaacgatttc catcctctat tgggtctaaa tatatttcat acttatttac
    13201 ataatcctct atttccttgt cacttaaata ctttgcacaa tcatgaatca ttcctgctaa
    13261 gtaagctttt tctttgtcac atccataaat ctcacataat ttttcagcac attttgcaac
    13321 attttttgaa tggctaagcc tccccactgg tagcatttga tttagtcttt ggttaatatt
    13381 ttctaagttc atttaatcat ctccatattg atatagttta ttttcatata aataattttc
    13441 tacattttcg ggtactaagt aacgtattga cttgccttca tttagctgtt cccttatata
    13501 tgtggacgat atatccagtg atggaacata aactgttata atgtcagcat tatacttctt
    13561 agttagtttt tctatagtct cttgtgacct taacaaacta attcctggtc tagttgcggc
    13621 tataaaagta gctaactcaa agtttttctt tacatctttc cactcttcaa cactacaaat
    13681 tgcatctgca cctgttataa aataaatgtc tgcatttttg tattttttct ttaagtattt
    13741 taatgtatct attgtataag tccttttctt tctttcgatt tctacttttg aaactaaaaa
    13801 atcttcgttg ctttcagttg ctagaagtgt catattatat ctatcatact tatctgtagt
    13861 gatacataat ttatgtggtg gatttcctga tggtataaag attattttgt caatgtcata
    13921 cttgtctcta ataaattctg ctgttgctaa atgagcatag tgtatagggt caaaagtacc
    13981 tcccaatata cctattttga ttttgccttt atggcgatta aatttctcta gtttttcagt
    14041 atttttcaac tctgccatct taactagatt ttcacttctc atattttgtc tccttttaat
    14101 gttataatac tattgtacca ttatttttta tttcttaaag cttataattt gttttttt
//
LOCUS       DAMJVI010000092        14078 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.097, whole genome shotgun sequence.
ACCESSION   DAMJVI010000092 DAMJVI010000000
VERSION     DAMJVI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14078
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.097"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            163..1314
                     /locus_tag="PC545_002862"
     CDS             163..1314
                     /locus_tag="PC545_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457619.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIAYEIVEFDETNF
                     EKDNDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGPIGYKKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLESLNYKVHVKNLKDQLKCNG
                     TKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDFELLKYMEFKQVENKYIIKYKEEELV
                     IENLDKLNKLFFEKNEEQYNEFKHLKNIYEFATKAFPVDFPWTANLNYQ"
     gene            1432..2070
                     /locus_tag="PC545_002863"
     CDS             1432..2070
                     /locus_tag="PC545_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HDF2457620.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINLLKGTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNMIEIDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            2071..3093
                     /locus_tag="PC545_002864"
     CDS             2071..3093
                     /locus_tag="PC545_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDF2457621.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLDTLRPDYDFPSVLTHTITDRDKLRKL
                     DVPDPYTSGRMPLNIESLSVISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEFIVKNLNKIYNEL
                     DCWKGMHICGDTRELLDNMLSCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRRYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDIDEL"
     gene            3083..3703
                     /locus_tag="PC545_002865"
     CDS             3083..3703
                     /locus_tag="PC545_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457622.1"
                     /translation="MNYNSNKNKYLLKDINLDDIFIDKNIVLKFLGYGNRKAPDTISK
                     IVEREIRNINDILDIKIYISEVDINKTPKECKKAFAVLYTIGDKIDFKMNDYMENCNM
                     MAGLALDKVGIVCLDCINEKIKMYLKGKYDSLKISHEIYPGDKDFEVERQKDIYNYVK
                     NKYNSIEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(3827..4513)
                     /locus_tag="PC545_002866"
     CDS             complement(3827..4513)
                     /locus_tag="PC545_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDF2457623.1"
                     /translation="MKTYKFIFFDADDTLFDFKKSESHAFKKLLSEFDLEFNFENYIE
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKISLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILQYLKKKYTIVIITNGISAVQKKRLENSKIKEYIDGMVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALIIGDSLTSDILGGINSGIDTCWLNSNNSINYTNHIPTYEI
                     NTLIELKKLL"
     gene            complement(4527..5072)
                     /locus_tag="PC545_002867"
     CDS             complement(4527..5072)
                     /locus_tag="PC545_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457624.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHDNRKKVILPGSKEVIYEILIPDSS
                     GNLEFAIMDLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLSKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            5300..6547
                     /locus_tag="PC545_002868"
     CDS             5300..6547
                     /locus_tag="PC545_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDF2457625.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMANGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALFSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILLAMLGGGKAGNIISPNPNT
                     IAVADNFFVNLSSVMMANIIPAIIGVVITVILASILINKGNKVQSYEILEQREDLPSL
                     FKSLCGPIIAIFLLFLGNVSPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIGALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIVNAGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6634..8628
                     /locus_tag="PC545_002869"
     CDS             6634..8628
                     /locus_tag="PC545_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2457626.1"
                     /translation="MNKKKIVIIGIIYSFLVVFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIIVDLINSLSIQIGRDFYFKP
                     NNWWKESFVNSIDDSIKFFDLIPSKERANKFIFTDPIYTLSANILKDKKSQDINSYMD
                     LKGKTVAIPEGDYSINFLKQKIQDINILLTPDIKTGVNHLMSGKVDAVVGDEPVLRYY
                     INNYGLSNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEQVIENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDFMGEMSYKIADLISMIKGVIENTFSENTN
                     KTSEIEIHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKMLQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNYCLFIDFKDNGEGIKEDALKDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNKEVTI"
     gene            8628..9950
                     /locus_tag="PC545_002870"
     CDS             8628..9950
                     /locus_tag="PC545_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HDF2457627.1"
                     /translation="MKILLVDDELEYGVVMKKILQKKGYLVDIALSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKSINRDIEVILVTGYGSIENAVDAMKRGALSYFIK
                     SNPIENLLEEVEKVKASKISVSVQKNNLEFTLESKNRDFNNIIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLTTLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIRP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKISEYNIK"
     gene            10229..11515
                     /locus_tag="PC545_002871"
     CDS             10229..11515
                     /locus_tag="PC545_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HDF2457628.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYISSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            11601..12908
                     /gene="grdB"
                     /locus_tag="PC545_002872"
     CDS             11601..12908
                     /gene="grdB"
                     /locus_tag="PC545_002872"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:12648..12650,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HDF2457629.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEESPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVITGMYKENPGVDMFKLDLHIVSTGNSAASLRKVVPIMTKLGLKLVR
                     GEEIGPPEEEGYIMRGIRKNFFHELRGSERAIDMLVKKMKGESFETEYPMPEFDRVTP
                     MKPVKDLSKIKLALVTSGGIVPIDNPDKIESSSATKYGIYDLTDMDSMSNKEFTTIHG
                     GYDRAYVLENPNLVVPLDVVRELEKEGVIGELVDYFITTTGTGTSVGNSKRFGEEFSK
                     KLLEDDVDSVILTSTUGTCTRCGATMVKEIERAGIAVVHICTVVPISLTIGANRIVPA
                     VGIPYPLGNPMLGEEESKKIRKNIVLKALNALTEDIEEQTVFE"
     gene            13186..13917
                     /locus_tag="PC545_002873"
     CDS             13186..13917
                     /locus_tag="PC545_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457630.1"
                     /translation="MIRKKVYILISIAICDDESYMLNHLKENIELYMKQKNLLYNIEL
                     FNKVEDLLCNHEKFNIVFLDIQMEEINGMQAARKLRSYGNECFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLDDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETVDYYETIENLERSLPTNFFRCHRSYIINLQYVYGYENGNAVMESGD
                     RIPVSRLRQQIFSKVMLEFMKNRRI"
BASE COUNT         5638 a         1400 c         2448 g         4592 t
ORIGIN      
        1 tataagttta actataaatt aggtttatat tttataaaaa tttgctaaaa aaaacaatta
       61 aaaattgtaa agaaaaaaga tataattaga actgtagtaa taaatgtttt attatagata
      121 atagagtttt aagtatctta aataatattt ggaggaatat gaatggaatt aagaccaaga
      181 agtgcaaaat tagaagaatt tgataaagta atagaattaa taaactatgt atttagaata
      241 tcaagaaatc ataaaccaac tatgatggaa gaatttcctc ttttactaag taaaaataat
      301 atagaaaata tgattataat atcagaagat gataaagtgg tttctgatgt aaactatcta
      361 attcaggatg tatctattca aggaaacaga ttgaaggttg cagctatagg tggagtatgt
      421 actcatccag attatgaaaa gagaggctat tcatctaaga tattggataa agtagaagaa
      481 aaaatgtttt atgatggtgt agatattgta attatatcag gaacaagaag tctatacagt
      541 agaagaaatt gtagtttggt aaagagtttt tataaatata caataaaacc tgaagatgta
      601 aaaatagcat atgaaatagt tgaatttgat gaaactaatt ttgaaaaaga taatgattta
      661 gataaaatga ttgaactata taatcaaaat agtactagat ttataagaac gagagatgag
      721 tttcaaaaac tattgcatgc agcaactatt gcctggggac caattggtta caagaaggta
      781 tttataaagg aaaataataa tataatagga tatttgataa taagaacaat aaaaaaagaa
      841 gattctactg taggagaagt tgctgaaata gggttaaact ctgtaaatgt tgaaaatata
      901 ttgaaatatg ttgcaaataa atttggatta gaatctttaa attataaagt acatgtgaaa
      961 aatcttaaag accagttgaa atgtaatggg acaaaatctt tggattatca acaaggaact
     1021 atgaaaataa taaattttac taagttgtgt gatagtttaa gaagttattt tagtcaatat
     1081 gtagattttg aattactaaa atacatggaa tttaaacaag ttgaaaataa atatattatt
     1141 aaatacaaag aagaagaact tgtaatagaa aatttggata agcttaataa actgtttttt
     1201 gaaaaaaatg aggaacagta caatgaattt aaacatctaa aaaatatata tgaatttgct
     1261 acaaaggcat ttcctgtaga ttttccatgg actgcaaatt taaattatca atagttttag
     1321 atgatattaa ataaaattga ttgataccta gagttagtcc ttgttaaata agagaaatta
     1381 ttggatattg aaaaagcaat tttgtaagta attagaaagg atgagtatta tatggacata
     1441 cttgatgata tagcagaatg tatattaaat atgggtgtag ataatataga gaatttagta
     1501 aaaattgcta tagataaaaa tattgatgtg gaagatattt atgaatatgg tttaaacaaa
     1561 ggtatgatag gagctttaga taagtttgag aataaggaat actatctttc agaagttata
     1621 gtatgcacag atgcattaaa taaaggtatt aatcttttga agggaactgg aaaagtaaag
     1681 aaaaaatcaa agggagtaat tctgatgtct gtagtagaag gagatactca tgagatagga
     1741 aaaaatattg taaaagtaat ggtagaagcc actggatata aggttattga cttaggtgta
     1801 aatagaaaaa gtgaagatat aatagaagaa gctataaaaa acaatgtaga tataataggc
     1861 ttatcttcaa tgatgacaac tacaatggaa aatatgaaat cggttataga cgaacttaat
     1921 atgattgaga tagataaaag accaaaagta ataattggtg gaggacctgt aagtatggag
     1981 tttgcagaag aaataggagc agatggatac agttcaaatg ctccaaaagc agttaaatta
     2041 ataaataaac ttataggagg tgaagtttaa atgttttcag atatgacttt acttcagtat
     2101 ataaatagtg aagatagagg tttttttctg cctgatatgg gtacaaatgg attgttctta
     2161 ataaataaaa aggcatatga agtatatgat agtgtagatt tgcaattaga attagcaaag
     2221 actatggaca agtattttga atcagatttt atatactcat tctgtgatgg gattacattt
     2281 tgtgaaactc tgggtttaga tacattaaga ccagattatg attttccaag tgtattgaca
     2341 catactataa ctgacagaga taaattaaga aaattagatg ttccagaccc ttatacgagt
     2401 ggacgtatgc cactcaatat agagagctta tcagttatat caaaaagtat taaaaaacca
     2461 ctatatgaat cgatacaagg tccatttaca cttgcaggtc aattagctgg agctactcaa
     2521 cttttaagat gtataataac tgataaaaaa tttgtagaag aattattgga atttacaact
     2581 gaattggtaa gaagatatgc tgtagctgca aataaagcag gagcaaaata tatttcaata
     2641 gcagagccaa catcagtaac cctaagtaaa gatagatttg atgaatttat agtaaaaaat
     2701 ttaaataaaa tctacaatga acttgattgt tggaaaggta tgcatatatg tggagacacc
     2761 agagaattat tagataatat gctaagttgt aatatagatg cagtaagttt agaccaaatt
     2821 ttagactatg aagaaattgc tccaatgatt cctagagata tagttttgat tggtaattta
     2881 gacccaataa aacttcttgg acgttctaag ccagataaga ttagaagaga gacactaaaa
     2941 ttgttaaaga agatgagaag atatgataac tttttatgtg attttggctg taactgctta
     3001 aataccactc cagtagaaaa tttacaagca gctataaagg caggaagaat tagttataaa
     3061 gaattagata ggattgatat tgatgaatta taatagtaat aaaaataaat atcttttaaa
     3121 ggatataaat ttagatgata tttttataga taagaatatt gtgctaaagt ttttggggta
     3181 tggaaataga aaagctccag atacaattag taaaatcgtt gaaagagaaa taagaaatat
     3241 aaatgatatt ttagatatca agatatatat aagtgaagtt gatattaata aaacacctaa
     3301 agaatgtaaa aaagcatttg ctgtactata tacaataggg gataaaattg attttaagat
     3361 gaatgactat atggaaaatt gcaatatgat ggcagggctg gcattggata aggtaggtat
     3421 agtatgttta gattgtatta atgagaaaat aaaaatgtat ttaaaaggaa agtatgatag
     3481 tctcaaaata tcgcatgaaa tatatccagg agataaagat tttgaagtag agagacaaaa
     3541 ggatatttat aactatgtaa aaaataaata taatagtata gaaatagaaa ttaatgatta
     3601 tcatcaacta tcccccataa aaagtgttgc aatgcttatt ttaatggggg atgaagaaaa
     3661 tttagaatca agatgtagta agtgtcctag aaaatgcttt tagttaattt tcaagactaa
     3721 gatttgtttt gtgtataaat tgcaataatt tttttggtca ttttatgtat aaactaaaaa
     3781 aattattgca aagtaatatt ctatttaatt tgaacagact tacaaactat agtagtttct
     3841 ttaattcaat aagtgtattt atttcataag ttggtatatg attagtataa tttatactgt
     3901 tattggaatt taaccaacaa gtatctattc ctgaatttat tccacctaag atatcagagg
     3961 ttaagctatc cccaattatt aaggcagaag atttatcatg acaattaaac tttttgagag
     4021 cgtatttaaa tatttcagga tttggttttg atattttcaa ctcttcagat acaaccattc
     4081 catcaatata ttcttttatt tttgaatttt caagtctttt cttttgaact gcagaaattc
     4141 catttgttat tataacaata gtatactttt ttttaagata ttgtaatata tcgattgcac
     4201 ctggaattaa aaatgtacat tcacctaaaa aatttaagta cattttgcta agagtttcag
     4261 aatcaacatc caaagatatt ttatttgcaa aaagttcaaa tctaagtaat ttaagttcat
     4321 tcaaagttat aatatttttt tcaaggtcga gccaaagctt atcacttata tttctatatg
     4381 actctatata attctcaaaa ttaaattcta aatcaaactc agatagaagt tttttaaaag
     4441 catgtgattc acttttctta aaatcaaata aagtatcatc tgcatcaaaa aatataaatt
     4501 tatatgtctt catggttttt actcctttac aagatgactg caaaaattac tttactttca
     4561 ctatttgagt cattttgcca tttatgtttt gttcctaaag gaacttttac actatctcct
     4621 ttggacaaag taaattcatc atcatccata aaaagtttaa cttctccctc caaaacatat
     4681 gcaatttcat ctccattatg agtaatccta tcaacacaag aagaagtgtt tggagctaaa
     4741 tccataattg caaattctaa attaccacta gagtctggaa ttaaaatctc atatataact
     4801 tctttgcttc caggtaaaat aacttttttt ctattatcat gtcttactat caagtttttt
     4861 ttatctacct cagatacaaa aaatgtaaat aaaggaacgt ttagagaaga ggctatagat
     4921 tttaatgaat ttacagatgg attagcaaga cctcgttcta tttggctcaa taatgatggt
     4981 gtaacatcag ctagttttgc taaatctcga atacttaagt tttgtttttt tcgaacttca
     5041 gctatttttt cccctaaatt taaatcattc atataaatat cctctctttt taatttaaaa
     5101 aatattaact taaaataatc aagtaaaatg attatgtagt atgaaattat tataacatat
     5161 agaattaggt tttgaaaaaa ctatgttcaa tataattaaa taaattaaaa tataattgac
     5221 aaagtggata cagaatactt aaaatatatt taaataaaat taagcaaaag atagttaatt
     5281 tatgatagga ggggatatta tgcaaataac gacttttgga gctatatttg gtttattaat
     5341 agcaattatt ttaattataa aaaaatttca agctgtatat agtttaatgc taggagcatt
     5401 tattggagga ttagttggag gagcaaatat aacacaaact gtagatttta tggcaaatgg
     5461 agctatgaat atatcaccat caattcttag agctttggca tcaggtgtat tagctggaag
     5521 tttaataaaa acaggggcag tagataaaat atcagaacag attgtaaaaa tatttggtga
     5581 aaagagggct ttattttcga ttgccatttc aactatggta cttgctggag taggcgtaaa
     5641 tttggatgta tctataataa ctgtagcacc tataggatta tatataggta gaaaattaaa
     5701 ttattctaaa ttgtcaatac tattagctat gttgggaggg ggtaaagcag gtaatataat
     5761 atcaccaaat ccaaatacta tagcagtagc agataatttt tttgtcaatc tctctagtgt
     5821 tatgatggca aatataattc cagcaattat tggagttgtt attacagtta ttttagcaag
     5881 tatattaata aataaaggaa ataaagtaca atcatatgaa attttagagc aaagagaaga
     5941 tttaccaagt ttatttaaat cattatgtgg tccaattata gctatatttc tattgttttt
     6001 aggaaatgta tctcctatag ttatagaccc aatgatagcc cttccaatag gaggaatcgt
     6061 aactctaatt gtcacaggaa acttgaacaa ttcaagagaa tatttagctt ttggattatc
     6121 taagatgcaa ggggtatgca tattattact tggaacaggg actatcgcag gaattataca
     6181 gatgtctgaa ttacaacaga gtacaatagg tgctttacaa tttttaaata tgccacaatt
     6241 tttattggca ccagtttcgg gtatacttat gtcattagca acagcatcat caacagctgg
     6301 tgctactata gcatcatcta cttttcatga tgctataata aatggtgggc tatcaccgat
     6361 ttcaggtgcc tcaatagtaa atgctgggtc tagtgtattc gagcaattac cacatggctc
     6421 actatttcat actagtgcag gtagtataaa catggatata ggtgaaagat ttaagttaat
     6481 tccatatgaa gcattgatag gaatagtaat gactataatt tcaacatcta ttcaattagt
     6541 tttataataa atataaatat ttaatgaaaa aagtaggatt ttgttattaa atattgaatt
     6601 tattatagta aaaaagaaga ttggagtttt aaagtgaata aaaaaaagat tgttattata
     6661 gggattattt attcattttt agtagtattt tcacttacaa atatgtatgt aaatatggag
     6721 tataatctaa atgtatttga atatattaag aagtcacttc catttacaga agaagaaaaa
     6781 aagtggttag aaaaacacaa gaatttgatt tacagctctg accaaagttc acctcctttg
     6841 agatataaag gtaaggaaga tggacagtat aagggaatta ttgttgattt aataaattct
     6901 ctgtcaattc aaattggtag agatttttat tttaaaccaa ataattggtg gaaagaatct
     6961 ttcgtaaatt caatagatga tagtataaag ttttttgact tgattccatc aaaagaaagg
     7021 gcaaataaat ttatatttac tgacccaata tacactttga gtgcaaatat attgaaagat
     7081 aaaaagtccc aagatataaa tagttacatg gatttaaagg ggaaaacagt agcaattcca
     7141 gaaggtgatt attctataaa ttttttaaag caaaaaattc aagatataaa cattttgttg
     7201 actccagata taaagactgg agtcaaccat ttaatgtctg gaaaagtaga cgcagttgtt
     7261 ggtgatgaac cagtattgcg atactatata aataattatg gactttcaaa taaatacagt
     7321 gttttaagta atcctatata tactaaaaaa gcagttcttg ctgtacctaa acaatatgaa
     7381 gagctggtta gtattttaaa taaaggcatt tttaaacttc agaaaaatgg agtgtataaa
     7441 gatttaaaga aaaaatggta ttctacgtat aatgaagttg atgatatatt gtatgagcga
     7501 ggaattgtgc ctagtattta tctttttata ggtataattt taatatcaat ttatgttttt
     7561 tatagctata catatttgtt aaagatagaa atcaaaagga aaacagaaca ggtaatagaa
     7621 aataaaaaaa ctttagaggc aacttttaat agtattacag atataataat gcttgtagat
     7681 gaaaataaca atatagtaga atcgaacaaa gtactatatg actttatggg tgaaatgagt
     7741 tataagatag cagatttgat tagcatgata aaaggtgtta ttgaaaatac cttctctgaa
     7801 aatactaata aaactagtga aatagaaata cacaataaaa ttttaaagat aaacactttt
     7861 cctgtagagt ataaaaaaaa taatactgag tatatagtag ttcttattaa agatataact
     7921 aatgataaaa tagtagaggc gaaattactt agagaaaaca aaatgatatc tataggacaa
     7981 ttagcttctg gggtagcaca tgagataaga aatcctttgg gtataataag aaataattgc
     8041 tatctcttaa aagataacgt tacaatggaa gaagtaaatg attgtgttaa atcaatagaa
     8101 agtaatgtag atagggcaag taatataata accaacttgt taaatttcgc aagaatatca
     8161 gatgataatt tagaacatat aaatataaga aattttattg aaaatatagt gaaacttcaa
     8221 tataaaatgt tacagttaaa gaatgttgaa ataaaaattg actgtgaaca taatttagta
     8281 tgctatataa atggagaatc gttaaaacat gtatttataa atttaatttc taattcaata
     8341 gatgcaatac atcaagatgg aaaaattatt atatactgtt atgaaaaaaa ttattgttta
     8401 tttatagatt ttaaagataa tggtgaagga ataaaagaag atgccctaaa ggacatattt
     8461 aatcccttct atacaacaaa accaattggt gagggaactg gtttaggttt atacattact
     8521 tataatgaga ttaaaaagaa taatggagat ataagtgttg aaagtaaatt gggggttgga
     8581 acttgttttc atataaaaat tccattaaat aaagaggtga ctatataatg aaaatactat
     8641 tagttgatga tgagctagaa tatggagtgg taatgaaaaa aattctacaa aagaagggct
     8701 atcttgtaga tatagcacta agtggtgaag aagctataaa tataattaaa aaagataaaa
     8761 attatgattt ggttttgtct gatgttatga tgaaaaatat ggatggagta cagctattag
     8821 atagaattaa atctattaat agagatatag aggttatatt agttacagga tatggaagta
     8881 ttgaaaatgc agttgatgct atgaaaagag gagctttatc ttattttata aaaagtaatc
     8941 ccattgaaaa tttattagaa gaagttgaaa aggtaaaggc aagtaaaata tctgtttctg
     9001 tacaaaaaaa taatctggaa tttactttgg aatctaaaaa tagagatttt aataatatca
     9061 taaaaatagc aaaaaaagct gcttgtaaag atgttaatat attaatcttg ggggaatctg
     9121 gtgtaggaaa agatatatta gctagatata tacatagcat aagtccaaga aagaatgaga
     9181 tatttgtacc tgtaaattgt tgttcttttt cagaaaatct attggagtct gagttgtttg
     9241 gtcatgaaaa aggttctttt acaggagcag tagatagtag aaaaggtaga tttgaacttt
     9301 cgaataaagg aactctattt ttggatgaaa taggagatat acctctaaat gttcaggtaa
     9361 aacttttgag gacacttgag gacaaatcta tagagaggat aggaagtaat aaatctatta
     9421 aagttgactt taggttaatt tgtgctatga ataaagaacc aaaagtggaa atatcaaatg
     9481 gaaatataag agaagatttt ttttatagaa taagtacaat aacaataact ataccaccgc
     9541 taagaaagag aagagaagac ttaactactc ttatagaatt tttcttaaat aagtaccaaa
     9601 ttgaacatga taaaaagata cactcgattg ataaggaagt aaaggatttt ttattaaatt
     9661 acaattatcc aggaaatata agagaattaa aaaatataat aaatagactt gtagttttaa
     9721 gtgaagaagg caatttatca aaagacaatt taaatttaat atcaaataat gtatacatag
     9781 atgataaaat aagtattaga cctttaagag aaataagaaa agagtttgaa tgtgagtata
     9841 ttgaaaaagt tttatctttg tgtggaaata atatttcaaa tacagctaaa aaattggaga
     9901 ttagtagaag acagttaacc aataaaatat cagagtataa tattaagtga gaaaaaaact
     9961 tctcattaag tgagaaaaaa atttctcaaa ctgtattaaa agataaacag aaaaaatctg
    10021 tttatctttt tttattataa ttttaaaaat taatatttac aataattatt gctaaattca
    10081 atattggtgt aaattaaaaa tgaaaatttc tatgacatta atacatagaa tatatatttt
    10141 aaataattgg catagaaaat gctttatata ttaataaacg taataaatac ttttaattgc
    10201 gattaaatat attaaggggg attttcatat gaagctagag ctagggaata tatttataaa
    10261 aaatgttgaa tttggagaaa agacagaagt aaaagatgga gtactatata ttagtagcga
    10321 agaaatagaa aagatagtat tagaagatga aaggattgta agtgtaaatg ttgaacttgc
    10381 aagacctggc gaatcaataa gaatagctcc agttaaagat gtaatagagc ctagagttaa
    10441 aattggagat gaaagcaaaa tatttcctgg aattataaat aaagtgaaga ctgtaggaag
    10501 tggaagaacc catgtacttt taggagcttg tgttgtaact tgtggaaata tagttggatt
    10561 tcaagaaggc gtaattgata tgagtggtcc tactgctaaa tatacacctt tttcaaaaac
    10621 aaataatata tgtattgtta taaaagcaaa ggatggtata gatactcatg attatgaaga
    10681 agcagctaga attgcaggat taaaaatagg tgcatatgta ggtgaggcag gaagagaagt
    10741 tgagccagat gaagtcattg tatatgagac aaagccatta ttaaaacaag taaaagaata
    10801 tccagacctg ccaacagtag catatgttca catgttacaa tcacaaggac ttttacatga
    10861 cacttattac tatggggtgg atgctaaaca gatagtgcct acatttatgt atccaactga
    10921 aataatggat ggagctataa tatctggaaa ctgtgtagca ccatgtgata aggttacaac
    10981 attccatcat ttaaataatc cagttataca tgacttatat aaaagacatg gaaaagattt
    11041 aaactttata ggagttattt tgactaatga gaacgtattc ttagtcgata aagagagaag
    11101 ctctgatatg gtagctaaat taatagaatt tttaggagtt gatggagttc tagtgacaga
    11161 agaaggttat gggaatccag atactgacct aatgatgaat tgtagaaaat gtagtgaagt
    11221 tggagcaaat gtagttttga taacggatga atttcctgga aaagatggca agagtcaatc
    11281 tatagcagac gctactaagg aagcagatgc agttgtttct tgtggtcaag gaaatctagt
    11341 ggttcatttc ccagcaatgg aaaaaattat aggaacttta gattatgttg aaatgatgat
    11401 aggtgggtat aaaggatgct taaatgaaga cggaagcatg gatgcagagc tacaaattat
    11461 aatagcatca actatagcaa atggctataa tcatttgaca gcaagatatt attaaataag
    11521 tatttgaata taaagttctg ttgatataat cattggtcta attgatgtaa caactataaa
    11581 gtgatttgga ggattaagag atgagtaaaa tcagagtggt tcattatata aacaatttct
    11641 ttgctggtat aggtggagag gaaaaagcag atattccgcc agaaaaaagg gctggtgtag
    11701 taggacctgg aatagcattt cagagtcaat ttaatgatga tgcagagata atctcaactg
    11761 taatttgcgg agatacgtat tttggtgaaa atattgagag tgcaactcaa gaacttttaa
    11821 aaatgataaa agaagaaagc cctgatttat ttattgcagg cccagcattt aatgcaggta
    11881 gatatggagt agcttgtggg actatttgta aggcagttga agaagagtta aatattcctg
    11941 taattacagg catgtataaa gaaaatccag gtgtagatat gtttaaatta gatttgcata
    12001 tagtgtctac aggaaattca gcagcaagtc ttagaaaggt agttccaata atgacaaaac
    12061 taggtcttaa attggttaga ggtgaggaaa taggacctcc tgaagaggaa ggatatataa
    12121 tgagaggaat aagaaaaaat ttcttccatg aacttagagg gtctgaaaga gcaattgata
    12181 tgttggttaa aaagatgaaa ggggaaagct ttgaaactga gtatccaatg cctgaatttg
    12241 atagagttac acctatgaaa cctgttaaag atttatctaa gataaaactt gccctagtaa
    12301 cttctggagg gatagttcca attgataatc cagacaaaat tgaatcttca agtgctacta
    12361 aatatggaat ttacgattta acagatatgg attctatgtc aaataaagaa tttacaacta
    12421 tacatggggg gtatgacaga gcatatgtcc ttgaaaatcc aaatttagtt gtaccactag
    12481 atgtagttag agagcttgaa aaagaaggtg taattggaga attagtagat tattttataa
    12541 ctactacagg aacaggaacg agtgttggca attctaagag atttggagaa gagttttcta
    12601 aaaaattgtt agaagatgat gtagattctg taattttaac ttctacatga ggtacttgta
    12661 ctcgttgtgg agcaacgatg gtaaaagaga ttgagagagc tggtatagca gttgttcata
    12721 tatgtacagt agtaccaata tcacttacaa taggagctaa tagaattgtt ccagcagtag
    12781 ggataccata tccacttgga aatcctatgt taggagaaga ggaaagtaaa aaaattagaa
    12841 agaatatagt tttaaaagct ttaaatgcac taacagaaga tatagaagaa caaacagtat
    12901 ttgaataata aaaatacaac acaacactga aataaaaaaa gatatcaaaa aataattttt
    12961 taaaattatt ttacgatatc ttttttttat taggtaaatt cgaaatttgc attttttaaa
    13021 attaaattta taaaagtgat aaattagttt attataatat tgatacacaa attcaaatta
    13081 gatatactat gaaaagcgag attattacat tttggaacaa atattcaaca tattagatca
    13141 tattaattga acagaaaaaa ttaactttat ataataatta gaataatgat aagaaagaag
    13201 gtgtatatat tgatttcaat tgccatttgt gatgatgaat cctatatgct aaatcattta
    13261 aaggaaaata tagaattata catgaagcag aagaatttat tatataatat agagcttttt
    13321 aataaagtag aagatttact atgcaatcat gaaaagttta atattgtatt tttagatata
    13381 cagatggaag agattaacgg aatgcaggca gctagaaaat taaggtcata tggtaatgag
    13441 tgctttataa ttttcgttac agttttaaat gaatttgtat ttgatgcttt tgaagtagat
    13501 gctataaatt atctgataaa acctctatct aatgagaaac tatataaaac tttggataaa
    13561 atagttacaa aacttgatga taatgaaagt aattatatca caatacaaaa aggttattca
    13621 tattggaaat taaaacttgc tgatatttta tattgtgaag ttataaaaag aaagatttat
    13681 gttcatttaa agaaagagac tgtagattat tatgagacta ttgaaaattt agagagaagt
    13741 ttaccaacaa atttttttag gtgtcatcgt agttatataa tcaatttaca atatgtatat
    13801 ggatatgaaa atggtaatgc agttatggaa agtggagata gaatacctgt ttctagacta
    13861 cgtcaacaaa tattttcaaa agttatgctt gagttcatga aaaataggag gatataagat
    13921 gattaattta ttttcttccg atttattttt aaagtacagt tttttatttt cttttattgt
    13981 atatagaatt ttaatggtaa tagtgcaata ctattttcta atcagtttta caaatgttaa
    14041 ttcaaaattt tggtgtttta ttttatattc gataattt
//
LOCUS       DAMJVI010000093        14012 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.098, whole genome shotgun sequence.
ACCESSION   DAMJVI010000093 DAMJVI010000000
VERSION     DAMJVI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14012
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.098"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            433..1083
                     /locus_tag="PC545_002874"
     CDS             433..1083
                     /locus_tag="PC545_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDF2457631.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEYIKEKYKNDTKELEKQIQVSSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAVVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            1286..1957
                     /locus_tag="PC545_002875"
     CDS             1286..1957
                     /locus_tag="PC545_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457632.1"
                     /translation="MAKILVVEDEKRMQNIIVEYMQKGGYTCITADDGVEALTILKSN
                     NIDLMILDIMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTDIPKNSLSLGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            1954..3084
                     /locus_tag="PC545_002876"
     CDS             1954..3084
                     /locus_tag="PC545_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457633.1"
                     /translation="MNSLRKIIEKLKNVDISNWIKQNFGLYSVRKKVFLLSKLSGALI
                     ILFYLVVEEFSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLIDEVDEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVADKMRMEQVV
                     ENLVINAKKHVVYNGNLDLSITCENGLLLFEIYNDGRFIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVNNRDRGVEFYFMIPIDE"
     gene            3515..4360
                     /locus_tag="PC545_002877"
     CDS             3515..4360
                     /locus_tag="PC545_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457634.1"
                     /translation="MIKVRELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGKEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMVIDKPKNL
                     KMKNSNNKVEVEFVYNGNRDIEIVDIQELESGITFKYDEILSVHSKEPTLEEVFIKYT
                     GRMLV"
     gene            4360..5388
                     /locus_tag="PC545_002878"
     CDS             4360..5388
                     /locus_tag="PC545_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457635.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSASPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKDMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFFICDLCFTVLLVV
                     FNIGFLEGIKILPQVLVQASILSIIMSLIGIGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYSQLKNAFFMNFSKELVYFVVCSLTIIVLFLLVKYIF
                     NKELVREG"
     gene            5391..6404
                     /locus_tag="PC545_002879"
     CDS             5391..6404
                     /locus_tag="PC545_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457636.1"
                     /translation="MIGLRYQLKSIKKDKMCIISFFLPVVMAILINFMGTIDISSLGE
                     IRFGIVKDTLPVQTEQWLKKYGMVSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVF
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEVLPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGTPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKGIVILAVHCVVWFMIYIILDKKRKLS
                     FSR"
     gene            complement(6921..8171)
                     /gene="cme"
                     /locus_tag="PC545_002880"
     CDS             complement(6921..8171)
                     /gene="cme"
                     /locus_tag="PC545_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HDF2457637.1"
                     /translation="MENKNTNSTWKKKVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTVCSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINSSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVISINNLIIIAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(8257..9003)
                     /locus_tag="PC545_002881"
     CDS             complement(8257..9003)
                     /locus_tag="PC545_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2457638.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELNLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAISLVNQYLQQLEIEKSN
                     AEEALTIVAQILNGNTPETNKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSSPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(9175..10197)
                     /locus_tag="PC545_002882"
     CDS             complement(9175..10197)
                     /locus_tag="PC545_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDF2457639.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKIVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKVIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSIGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGTGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIID"
     gene            complement(10197..12617)
                     /locus_tag="PC545_002883"
     CDS             complement(10197..12617)
                     /locus_tag="PC545_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDF2457640.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLRPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEIRNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDDFLKLLINQGNLNTFGTNLVNDILSKTQHLIESRLGHLSLYREM
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSLKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGVEGGQLVYQGDLEGLLQSDKSLTGQYLSGKYPMPN
                     RTSNKNITHTDKIPCITIQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQINSSESEIGEEDDYAVVETIADRLIGTKHISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLEQGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSECQGKRFNDESLSIKYKNKNIHDILNMSVSESVHFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRIKLAKEIGKKRNGNILYVLDEPTTGLSLYDTSK
                     LIQLLDELVENGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(12703..13623)
                     /locus_tag="PC545_002884"
     CDS             complement(12703..13623)
                     /locus_tag="PC545_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2457641.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLSLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTVTDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNGNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
BASE COUNT         4961 a         1812 c         2200 g         5039 t
ORIGIN      
        1 gaataaaaat gataaatata ataatatata tatttacatt aggagttttc tatactataa
       61 ttatttaaaa ctaaatataa atgttggaat tatttaggac ttgaaattgt ctaaaaataa
      121 atacaagtga tgaagaggtg atgtgatgag attagatatg gatagtatgt tatatgtact
      181 aatttatatg acattattgg tatctatcat tggaagtata ttttattttt gtaaagacct
      241 cttaaatcat aaaattaagt atgttttaat aagagacata tttgtaaata gaattacata
      301 ttctgaagaa cgactgccta aacagtatat agtttttcag aaatatgata tttggaggta
      361 ttgtagtttg ttttaatttg aaatgaatct attataaatt ctaaagttta aaaataaaaa
      421 ggagagaata aaatggattt catttcaagt atattgcaag gattattaag tatattattt
      481 aattttacag gtgattttgg aatttctatt gtacttataa ctttaatagt taaattagta
      541 cttatgccac tatcattaaa acaaagattt tctgtaaaaa aacagcaaga attagctgaa
      601 aaaatggaat atataaaaga aaagtataag aatgatacta aagaattgga aaagcaaata
      661 caagtaagtt ctgtagaaag tatgaagagt atgctgggat gtcttatagt tttggttcaa
      721 atgccagtag tatatgcttt atatcatgta tgtttagata tgacaaagga atttacaaca
      781 attataattc catggattgc taatttaggt acatttgata atttgtttat attaccttgt
      841 atatatacat taattatgtt agcttcaaat ttagtgaatt atataccata tttaaaaata
      901 aattctcaag taaagtttaa caaacaaatg gctgtggtga ctaccataac aagtcttata
      961 ctaactgtta gaactccagt agccataggt ctgtacttta taacaagtgc aatttattca
     1021 ttaattgaag atatttgctt tagaatatat tttaatcgaa aaaatagaat agtgacaaaa
     1081 taatataatt attattagaa atattttagc ctgctaacat agaaaaagtt agtaggctat
     1141 ttttatctat gatatcaatt ccattaagaa tagtctaaaa aatactatca aaatgctata
     1201 aaaaatacta taaaaaaata gttgaaaaat aaataaatta atataaacta aaataaagcc
     1261 tacaaaaaag aggtgataaa tagaaatggc taaaatacta gttgtagaag atgaaaaaag
     1321 aatgcaaaat attattgtag aatatatgca aaagggagga tatacttgta taactgctga
     1381 tgatggagtc gaggcactta caatattaaa gagcaacaat atagatttaa tgatattgga
     1441 tattatgatg ccgtatttag atggattttc agtgtgtaga gtttcacgtg aaatgactaa
     1501 tatacctatt attatattga cagctaaagg tgaagaggag gataaattaa agggttatga
     1561 atatggcgca gatgattata taacaaaacc atttagccca aaagtacttt tagcaaaagt
     1621 taatgctttg ttacgtagat acactactga tattcccaaa aattcattga gtttaggaaa
     1681 gatttttatt atggtagcat caagacaagt gtatgtagaa gataaattga ttgatttaac
     1741 atataaagaa tttgaattat tgcgtttatt tatggaaaac cctaatcaag ttttttccag
     1801 agagaaactt ttaaattgta tatggggata tgattttgaa ggaaacacac gaactgtaga
     1861 tacacatata aaaaccttac gcaaaaagct tggtagtgaa ggacaccata tagttacatt
     1921 aattcgttca ggatataagt ttgaggttaa ggaatgaata gtttaagaaa gattatagaa
     1981 aaactgaaaa atgtagatat ttcaaattgg attaaacaaa attttggact atattcagtt
     2041 agaaaaaaag tgtttttact ttctaaatta tcaggagcgt taattatatt attttatctg
     2101 gttgttgaag aattttcgat aaactctaaa tttgaatttt ggatttggtt tataatactg
     2161 gttatattta ttatgagtat tgatttctta ttgggaaaat ttatttcaga accaatcaca
     2221 tctataaata aatctgcaaa aagtatgtcc caacttgatt tttctaatcc atgtactgta
     2281 aatactaatg atgaatttgg agaactttct aggagcctga atacaatgtc tacaaatcta
     2341 caacaggcat tatcagacct agaaagtgca aatattcaac ttgaaaagga tgtaaataag
     2401 gaaagaatgc tattggaaca aagaaaggag ttggtagata caatttcaca tgaaatgaag
     2461 actccacttg gcattatacg tgcgtataca gaggggctta tagatgaagt tgatgaagaa
     2521 aaaaagaaaa attatatgaa tgttataata gaagaaactg atagaatgaa caatatgatt
     2581 gtatcattat tagacttatc tgcattggaa gcaggagttt ctaaacttaa cccagagcgt
     2641 tttgatttta tagagtttgt tgaaactgtg gcaggtcgtc ttttaattga tataccagat
     2701 gccaattttt attttacata tgatttgcca gagtatgaag tttttgtagt ggcagataaa
     2761 atgagaatgg aacaagtagt agagaatcta gttattaatg caaagaagca tgttgtatat
     2821 aatggtaatt tagatttatc gattacatgt gaaaatggct tgcttttatt tgaaatttat
     2881 aatgatggta ggtttataga gcaagatgag atttcaaaga tttggtctaa gttttatcgt
     2941 agtgttcaat ctcagcgtac aggaggttca ggtctagggc ttgcaattgt ttcacaaata
     3001 cttacaatgc aagggttaga atatggagtg aataatcgtg atagaggagt agagttttat
     3061 tttatgattc caattgatga ataatttagt attaatttat aaattataag tatctgatgt
     3121 aaaatatttt tatttgtata cgtaagtatt taaagagaat aaaataaaaa aacttaatga
     3181 aaaagcttaa tgaaaaagtc taataaaaag cttaatgaaa agcttaataa aaagtcttgt
     3241 tttcctgatg aaaacaagat tttttattaa gctttttatt atttcacttc gatttcacaa
     3301 gagagacact ctgatttcac caaatagaaa taaaataggt taaataaatt aaaaaataga
     3361 agcgtaatta aataaggtta caagggggag tacttatgaa atggtataca tggttgacta
     3421 ttatattgat aatattgctt tctattccgc ttaaaattaa atttataaaa tggtgggata
     3481 gacgacaaaa aaatgataaa ggaggttatg atagatgata aaagtaagag agttaaaatt
     3541 ttcttatgga aaagataaac agatattgca tggacttaat tttgaagtaa aagaaggaga
     3601 aatctttgga ttccttggac ctaatggttc aggaaaatcc acaacacaga aaatcttaaa
     3661 tggtgtttta aaaggttaca atggtcacgt atccattttt ggtaaggaag ttaaagcata
     3721 cacagaatct ttatatcaaa agattggtgt tttatttgaa tttccttatt tatataccaa
     3781 tttaagtgca attgataatt tagaatattt ttcgtctttt tatcctaaaa atcaaaggag
     3841 agacatatat gaactcttag atttgttaga gtttaagaaa gagtttataa ataagcctgt
     3901 atcatcctac tcaaaaggta tgaagcaacg tattagcatg gcaagagcat tggtaagtaa
     3961 tccaaagttg ttatttttag atgagcctac tagtggactt gacccatctg gagctgtact
     4021 atttcgtaag attattgaag aagaacgaaa aaaaggaaca actatctttt tgactactca
     4081 taatatgcta gatgcagatt taatgtgtaa caatgtggct tttattgcag atggaaagat
     4141 tatggtaata gataaaccca aaaatttaaa aatgaaaaat agcaataata aagttgaagt
     4201 ggaatttgta tataatggaa atcgagatat tgaaattgta gatatacaag agttagaatc
     4261 tggaataact tttaaatatg atgaaatctt aagtgtacat tcaaaagaac ctacactaga
     4321 agaagttttt attaaataca caggaaggat gttagtgtaa tgtattctaa attttttacc
     4381 ttagtaaaaa aagatttttg gatgatgatt tatggtaaat tttttgtgct gacacttggc
     4441 tctctcatac tttattcgtg ttatattaat tttggttatg taaagtttat ggatgaagat
     4501 agttatcaca ataatgtata tctatataat ccagagaata taagcatgag tgcatctcca
     4561 ttaattaatc aagtttcttc tataggtgaa ttaaaagcaa aacttgatga agatacaagt
     4621 gcagtttgta ttgatatgag ttccagtaag ccgaaagtaa ttttacatga aagtattaaa
     4681 ggagttgaca attatagagc tgactatgca ttgtcattat tatcatcaga aaaagaccat
     4741 aatagcaaaa tatcagacaa caatgcaata attcaaatta atgataagga tacatctgag
     4801 ataaaacaaa gaaaagacat gacttgtgag attttattct ttgaaattgt agctattgga
     4861 tttttaggga ttgcctcttt attatttaaa gaaaagcaga tgggagtaat gcgtattact
     4921 ggtattttac ctgtaaaaag aagtcttttt atattatcaa agatatttgc tttttttata
     4981 tgtgacctat gttttacagt tcttttagta gtatttaata ttggattttt agaaggaata
     5041 aaaatacttc cacaagtatt agttcaagct agtattttat caattattat gtctttgatt
     5101 ggaataggat gctcaatgtt attgagagat tttaagcaat tttctcttat atatcttgtg
     5161 gttgctgtat tcataactac acctatattt ctatctgcaa atacatcttt tgaaatagat
     5221 tggattaaat atcatccctt ctatcatgtt tatagtcagc ttaaaaatgc gtttttcatg
     5281 aatttttcaa aagaattagt ttattttgta gtgtgttctt tgacaataat agttctattt
     5341 ttacttgtaa aatatatttt taataaagaa ttggttaggg agggataatc ttgataggat
     5401 tacgttacca gttaaagagt ataaaaaaag ataaaatgtg tataatttcc ttctttttac
     5461 cagttgttat ggcgatttta ataaatttta tgggaacaat tgatatatct tcattaggag
     5521 agatacgttt tggtatagtg aaggatacac ttcctgtaca aacagagcag tggttaaaga
     5581 agtatggtat ggtaagtgta tatgacacaa aaatgcattt aatacgtgaa attaaggata
     5641 ttaaaacgga cacaattgga gttgttagag atggtaaagg aataaaaact gtgttttatg
     5701 gaaatgagat taatgctata aaaaatgttg gaaagacatt gccagagatg tttagactaa
     5761 agggtgattt aaaagatgtt gaggttacaa tattgcctaa agatagtatt ttaaaagagt
     5821 ttcaaaatgt ttttattgct atgattttaa ttactgctat gtttatggga tgcaccttta
     5881 atgcaatgaa tattatatcg gaaaaagaag atggaatatc atttataaat gaagttttgc
     5941 cacagagtag aagtcagtat attatacaaa aagtattttt gggtctttta tgtgggtgtt
     6001 tatcttcaat gataactgta tttatatgtt tggatatttc aatattagat atgataatga
     6061 tgttgttact cgttgtattt tcagcattta tttcatcact tataggttta tttattggaa
     6121 agttgtcaga aggtcttatg gtaggaattg tttatattaa aatagtaatg attttattta
     6181 taggtactcc tttgttaata tatcttctag gggttaatac cagtggtata gggaatttat
     6241 gctatataat gccttcagtt gctacattta atggtattat ggagattatt aatggtaatg
     6301 tcattattat caaaggtatt gtaattttgg ctgtacattg tgtagtatgg tttatgatat
     6361 atattatttt agataaaaaa agaaagttat cttttagtag ataactcagt ataattataa
     6421 ttgggacaat ttaataggtg atttaatata ctttgtatag tgtaatttat gtagtgcaat
     6481 ttgtatactt taatttatgt agtgtaatag ttagttttat tttataatta ttcaatataa
     6541 tttagtaaga agttcagtac aattagagct acttagtgga aaacttaata ggtacttaaa
     6601 ctatataaat actttatttt cctccatttg tactataaat ttaatttatg atataaatgg
     6661 aggtattatt atatgatttg ataaatttca tcattataac ctacaagtaa aggtattatt
     6721 atataaagtt aataaagata gtatcaaaat attatttgct ataaagagag ggactcaaaa
     6781 taaagataaa cctatttttc aatgttgaaa tgataaaata tatttgattt catataaaaa
     6841 attttcttca taaattaaaa agtccatttt aagccccttc atttttaacc agataaatcg
     6901 agttaaacta tttaaatata ttactcggat tcatttgact tagatattcc ttgcttgtaa
     6961 aattgttttg aataggggat agccaaagca aaaaataaaa gtataatccc tgctatgatt
     7021 attaaattat taatagaaat tacatctgcc attggtccga aaatcatcat accaagagga
     7081 ataaaaccag tatatataat aataggtaag ctaagaactc tacctagcat ttgtggtgct
     7141 acatgctctt gcaacatagt catagctgat gcttgaatga ttggaattga aaaattcgta
     7201 aaaaacataa atataaaaaa ttgccaagct tgtgttacaa gacctagaat aattgaaaaa
     7261 atagcatagg ataatattgc tagaaaaaag gtcttatttc tatttttaaa accaccaaaa
     7321 actcctataa tcaatccacc taaaaccata cctaaaaatc caatcatttc aataatagac
     7381 aggtatatat aacccttgcc aaatgttcgt tcaataagaa gtcctataag aaatcctgat
     7441 ggaacacaaa gaagcatgaa tgctccataa gtaaaaagca cttttctaat gaacttatct
     7501 gaaaaacagt actttatccc tgataccata tctgcaaaaa atgagacttt ttcagtttgt
     7561 tttggtatat catgtttagg aattttgatg aatattaata atacaatagc aataattgca
     7621 gtaaatacat caattaaaag aacttgattt attttattac caaatccaag tattgcagca
     7681 gctacaatag gaactataaa ttgtactata gactgtagac tactatttat accattaaaa
     7741 cgcattaaat gttcttctgg tacaagttga ggaatcattg cgtttacagc aggtgtttgt
     7801 acaccacttc ctagtgaacg tataacagat attactgata tggctattaa ttcacttgtt
     7861 gtgccatttg atgacatcat aaatagaatt agaactaatg tagatatagc aattgttgaa
     7921 tctgatacaa ttatgagtac ctttcgattg tagtagtctg cccatacacc agcaaaaaaa
     7981 gaaattatca tttgaggtat aaaggagcat acagttaaaa ctgttaccca tacacctgat
     8041 gatgtctgaa gtgtgacata ccatatcatt gccatttgta caagtgatga gccaaataag
     8101 gaaatatatt gacttgctaa aaaaagaatg acttttttct tccatgtaga attagtattt
     8161 ttattttcca tcctattcct cctttgattt gtgtgttgaa tttttcttgc tcttaaggat
     8221 aacacacaaa tcaaagtctg gagttagagt ggagggctag gaaaattgtt ttttcataat
     8281 ttgaagatga gatttcatgg tttttgcatt atgttcagct tctttcaatg aagttatcag
     8341 tttgtcacac actgagataa tttcgtcatc atcttttgga gtgtcaagga cagttttaat
     8401 attagtatta ggactagaag ataaagcatt taacattcgt aaaatagctg aaagagaata
     8461 gtttgcacaa cgaagtgcgc ttataatttt aagtcgtttt atatcttcat cagtatatac
     8521 acgataacca ttttgttttc tttttacttt taacaaacca ttcatttccc aatttcttaa
     8581 agtatctatt gtaacgttta agtgttctgc agtttctttt ctagttaaaa agagtttatt
     8641 tgtttctgga gtatttccat ttaaaatctg tgcaacaata gtaagtgctt cctcggcatt
     8701 agatttttct atttctaatt gttgcaagta ttgatttaca agactaatag ccttatcaaa
     8761 gtccctattc gctgatgttc ttacaatctc aagggcatct tttcttaatc ccctttgtac
     8821 cacttcaatt ttcaaggctg tacgagcaaa tctaaattgt tctatatgaa aatcattaaa
     8881 gatacgataa ccattggtat tacgcttagg tttaggtatt aagtttagtt cttcatataa
     8941 acgtactgta ttaggatgta caccaattaa atctgctacc tctgatgtcg tgtaagtgtc
     9001 cattagtatt caactccttt tagatatata gtattagtca taataacacc aaacaaaagt
     9061 ctggtgttat aatggagggt ttggctatct aagagtaatt agtttatgaa agagtaatta
     9121 gtttatgaaa gagtaattag tttatgaaag agtaattagt ttatgaaaga gtaattaatc
     9181 tattatagaa gtaagtatat atttttcaat gaactcccat ctatcacgag gtttaaacac
     9241 tattttagat ggtatggcta ttactaagtc atattgtgga atacaacaaa tagcatttcc
     9301 tccagttccc attgctgaat accacaaaac accatctttt ttacatagcc accacagata
     9361 accgtattca ttactattca ttttaactga ttcttcaatc cacgatttag atataatacg
     9421 tttattattc caaataccat catttaaata cagaaaacca aatcgcaaca tatctctagc
     9481 actcaatgta agtccccagc cacctataga ataaccctgt ctatcttttg cccaaccctt
     9541 aagatttttt ccaaagaaat cttcaaaacc aaaatctttc atatcataat ctggtataac
     9601 tttcatacca ataggtttaa ataagtactc attagcaaat tcacgagcag attttccagt
     9661 agtttttgtt ataatagctg aaagtaggtg cgttccagct gaagaatatt tgaaagtacc
     9721 aatttcgcct ttttgaccaa gttgattcaa ggtatatttt atccaatctt tttgtttgca
     9781 aagtctttct aaagactcag tcatattttt aaaagggtat ggtgcagtca ttgtaagtag
     9841 gtgacgtata gttactgctt ttttacatat ttcagaagga ctacaagtat attcaggaaa
     9901 aaaatcaaca accttttggt caacatcttt tatatatcct ttatctattg caatacctat
     9961 taaggctgat ataatacttt ttgtaactga tgcaacatga aaagtatcat cagcattata
    10021 tccattatag tacttttcaa agacaatttt tccttttctt gcaataatta taccatttat
    10081 attgctatat tgtgagatta ttaaaggttc aagttgtgaa agcttttctg aactcatacc
    10141 aacacttgaa gggtctactt tttcaaaaat taagtctaag caattgtttt tattcattaa
    10201 agatacctcc ctgttataga ttttggattt tcttttagag tttttggaga accttctgca
    10261 attatataac ctcctttgtc accaccttct ggaccaagct caacaatcca gtcacaaaca
    10321 tttaatacat ctatatcatg ttcaacaaca attacagaat ttccattttc aaccaattca
    10381 tctaataatt gaatcaactt agaagtatca tatagactta gaccagtagt tggctcatcc
    10441 aatacataaa ggatattacc atttcgtttt ttaccaattt cttttgcaag tttgattctt
    10501 tgggattcac caccacttaa agtcggtgta ggttggccta attttatata ccccatacct
    10561 atttgttcta atacatttaa gtgtgaaaca atatttggaa tatctttgaa gaaatgtaca
    10621 gattcagaaa cactcatatt taaaatatca tgtatatttt tatttttata tttaatagat
    10681 aaagattcat cattaaatct ttttccctga cattcactgc atatatggtc tatacttaga
    10741 ttatttccca accagacctt ttcatatcca ctaccactac atttaggaca agcccctttg
    10801 gagttaaatg agaaatgacc agctgtaaat ccttgttcta acgattctgg ctgacctgca
    10861 aatagtgtac gtatcttatc ccatatacca atataagaag caggagttga gttcatattt
    10921 ttgcctatag gagattgaga tatctctgaa tatccagaaa tatgtttagt accaatcagt
    10981 ctatctgcaa tagtttcaac aactgcataa tcgtcttcct cacctatttc actctctgaa
    11041 gaatttattt gattattgat agtatggtta ttaaattggc ttctaagaag aggaagtagg
    11101 gtgtctgata taagtgaact cttgccacta cctgattttc cagatattcc aaccatagca
    11161 cccaaaggta atgatacagt tacattttta agattatttg tatttgcatt ttgaatagtt
    11221 atacaaggta ttttatcagt atgtgtaatg tttttattgc ttgttctatt aggcattgga
    11281 tattttccag atagatactg tccagtaagt gatttgtcgg attgaagtag accttctaaa
    11341 tctccttgat atactaattg accaccttca acaccagctt ttggaccaat atcaataata
    11401 tgttctgcca tttcaattgt gtttttatca tgctcaacta ctatgaccgt attgcctaaa
    11461 tctttaagtt tctttagtga ttttaagatg tctatttttt ctgattcatg caatccagct
    11521 gttggttcat caagcacata aattaatgag tccatctctg aatcgagatg tgaatttaaa
    11581 aatagtcttt gcatttcccc accactcaaa gttgacattt ctcggtataa ggatagatga
    11641 cctaatcgtg attctattaa atgttgagtt ttggataaaa tatcatttac aagatttgta
    11701 ccaaatgtgt ttaaattacc ttgatttatt aagagtttaa ggaaatcatc aatttctaaa
    11761 atcgtcattt ttccaagttc tccaatatgt tttccgttta aaagtactct acgtgcttct
    11821 tcaccaattt taaaaccatt gcaatctgaa caggcagtca tagtataaac tccatttaaa
    11881 tcttcgcccc ttttgtaacg attgtgaaat acctttgata aagaggaact ttttttacca
    11941 ttttcataat gaccatagag tacttcattt cttatttctt caggtagttg taagaattgt
    12001 atgtctaaat aatcattgaa ttttttagaa aatactctca aatatccagg tgtaatcttt
    12061 gctctatcta agacttgttt tagggtatca tgcttatcaa ggactaattt ttcaatatta
    12121 atctcaaaat atgcaccatg tccagagcac ttcatacaca ttccatctga agagttgtat
    12181 gagaaataac ttggtcttaa tcgttcttcc tcatatccac aattattgca aattagatta
    12241 tcgcttacag gagtatcaca catagtacac attatttgac cttccactga atacaatagt
    12301 gtaagcatgt tgagaatatt ggttcttgtg cctactgttg aacgaggatt gctctggcga
    12361 ataatatttt gttgaacagc tatagtggga gctaaacctt gaatattatc aaacttatct
    12421 tcactatcaa tacctgcaag tatacctaaa gattgcagat attgtttacg cccttcctca
    12481 aatacaatgt caaacataag gctagactta cctgagccac tgacacctgt tgcaacaaca
    12541 agcttatctt ttggaataga aacatctata tttttaagat tgtgtatctt tgctccatta
    12601 atttttattt ctttcattgg ttcacctcat tttatcaatt ttatcaaacc gtaaaatagg
    12661 gatatattaa tccttaatac aagaatagat taggtgaggt tattaaaaag gttctatagg
    12721 taaatatata tcgaccaagt gtctattttg agggtgattg tgtgggttgt taagatatac
    12781 ttcaaaaggg aaggaggcac gtagcttata attaccattt gatagccatt cattatacat
    12841 aaaatcccac gcatcactat attcagattt tgaaatatca aaatgtccca caagatattt
    12901 tccagaaggt attatcatat tgccaagatt gtcattctct ttgacaacaa tgttatctgg
    12961 aatggtcatg caaatactag tccttaattg atgctcctgt gtaaattgtg gattatcatg
    13021 ataaatggct aatatttgtg tgttttcaag tccaagtaga ttctgctcac ttgcatatgt
    13081 gaataaccgt tctaaaagtt ttgggaaagt actggttaga ttaccatatg aaccagtata
    13141 tctagtgtaa atagtattca tattatcaag ttctagaatt tcaatttcac ctttaacctc
    13201 tctagtattc ttacatttag gttttgatat gcttctattg tactgagaaa ttttatatgg
    13261 ttctttgcaa ttcttgctat aattgtttct atattttata gggcttattt tatagtaatt
    13321 tttaaatgct ctcgagaaaa ctgctgaatc agtaaagccg aagtagtgtg caatatcagt
    13381 aacagtcatg tcagttctat gaataagaaa agcagttgca ttttctagtt ttagcctatt
    13441 tacatattgt gccaaagttt ctttttctat tgctttgaag attctatgaa aatgaaattt
    13501 tgaaaaacct gctatattag aaagttcatc aagagaaaga ggttcatgta aatgttcttc
    13561 tatatagtct tgcactttgc aaatacgatt tagatattct tttttacttt tagcatttgt
    13621 catatatagc cctccttatt gtgtaaaatg tgtatcaatt aatttatagc aatatagtaa
    13681 taaaatttta tgtttttgta atttatgata aataagatag cgtttaattt tttataattg
    13741 ataaaagata taagatgcct tatatttgtc tttgcatata agtatagtct tcgtaataat
    13801 tagtatacca tagtgattta tttttaattg tgtatttata taaatacaca acttatgata
    13861 tggacgaatt attatgcaaa taagctgtaa ttaaataaat tgcaatataa accagttgaa
    13921 acataagtta gggaaatatt atcaaagtta ttacttagca taagagtata gatggaataa
    13981 aataagatta aaaaattaca tactaaattt aa
//
LOCUS       DAMJVI010000094        13827 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.099, whole genome shotgun sequence.
ACCESSION   DAMJVI010000094 DAMJVI010000000
VERSION     DAMJVI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.099"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            211..351
                     /locus_tag="PC545_002885"
     CDS             211..351
                     /locus_tag="PC545_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457642.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(526..846)
                     /locus_tag="PC545_002886"
     CDS             complement(526..846)
                     /locus_tag="PC545_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDF2457643.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NMDK"
     gene            complement(966..1703)
                     /locus_tag="PC545_002887"
     CDS             complement(966..1703)
                     /locus_tag="PC545_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDF2457644.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDVITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGDYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            1918..3741
                     /gene="acd"
                     /locus_tag="PC545_002888"
     CDS             1918..3741
                     /gene="acd"
                     /locus_tag="PC545_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HDF2457645.1"
                     /translation="MKKKAALATLAMLPLGVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKSNSDSLRTSTNKEKQVI
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLPKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDNSSQSNDKKNTDKVVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADTSNNNSNSNSDNSSNSNGNNSSSSGQVNGM
                     SGISGAKIDYKSLSYTLESHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSS
                     FVNASSSDIEYYLNPKNFTNTTKGMMQFLKINSYRDGISESSLNSYLNGLSSSVFKNQ
                     GAAFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFG
                     IGAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYVNSSKYNQNTIYKMKWNYD
                     YTWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            3956..8254
                     /locus_tag="PC545_002889"
     CDS             3956..8254
                     /locus_tag="PC545_002889"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDF2457646.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKVNKERLIRINDREMEEKIRALEIGKVNNCEENEEESYVIKSDVDENLIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRIEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHSAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEDDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSIGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFDKIPVATGDVHFLDKHEA
                     VLRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIEEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIVVGEKTLLPPMAAIQGLG
                     GNAAINIQNERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            8458..8919
                     /locus_tag="PC545_002890"
     CDS             8458..8919
                     /locus_tag="PC545_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDF2457647.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIANGKEMEFNRKDVAIVRLAIKF"
     gene            8949..10103
                     /gene="nusA"
                     /locus_tag="PC545_002891"
     CDS             8949..10103
                     /gene="nusA"
                     /locus_tag="PC545_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDF2457648.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKEIEAEDVEAEFIDQEELDTSEDNFSEEE"
     gene            10110..10391
                     /locus_tag="PC545_002892"
     CDS             10110..10391
                     /locus_tag="PC545_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDF2457649.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            10381..10692
                     /locus_tag="PC545_002893"
     CDS             10381..10692
                     /locus_tag="PC545_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HDF2457650.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            10714..12654
                     /gene="infB"
                     /locus_tag="PC545_002894"
     CDS             10714..12654
                     /gene="infB"
                     /locus_tag="PC545_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDF2457651.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGEELE
                     LALEMLGEDLSQENGNVIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            12697..13077
                     /gene="rbfA"
                     /locus_tag="PC545_002895"
     CDS             12697..13077
                     /gene="rbfA"
                     /locus_tag="PC545_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDF2457652.1"
                     /translation="MASYNRTRRIAEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKLDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            13058..13735
                     /locus_tag="PC545_002896"
                     /pseudo
     CDS             13058..13735
                     /locus_tag="PC545_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
BASE COUNT         5522 a         1568 c         2591 g         4146 t
ORIGIN      
        1 ctcaatttaa tatataaaaa agataataat agttagattt tttatatgaa ttttaatagg
       61 taaaatatcc tattgttttg tagatatatc ctaattatgt caaaaaataa aaaaattttt
      121 atttattaga gtttttggaa ataataataa cagatattat aattaattaa cagtttttgt
      181 aagtatttga caatagagag ggtgtaacaa atgggaataa tagagtcagc atcaaaattg
      241 gctgagatgg ttcatttatt ggctgttgaa aaggggataa ctgatataga agcatgggat
      301 gaagctgtga aagaatacag caaaatttat gaagaaagaa gaaatgaata gaatgataaa
      361 aaatgaaaac ttgatgataa aaaataactt tatttaaaga aattcaattt ttatagaagt
      421 attaaaaata atataaataa aaagatggta tttcaaatgt aagtaaaaaa tcattttggg
      481 ataccatcct tattatttat tttaataata tataagtaat aaatcttact tatccatatt
      541 ttttatgtaa cctctttttt ctaacttttt tatatgctcc ttacgtattt catcaagatt
      601 aataccatat tgttcttcta gcttaaacat catagtaaca catgtctgcg ctacatccat
      661 tagttcctta gcagttttct ttataatatc aacttcatct aaatcattat tttctccact
      721 tagatttctg aatttaccta tacattcagc taattcacct tgttcttcac ccattttgat
      781 aaatgtagat tctagtgttg ggtctaaatt attaagctct ggtaaagata tagtttttag
      841 tttcataaaa gctcctttca agttattcgt atttatacta aaacatcata acaaaaataa
      901 tactaaaaaa aaactttaaa ttcaacataa aaacttacat atataataaa aatcatgaaa
      961 taaatctata tttgtcttaa tacagcctca tataaaactt ctccaatagg tttatttatt
     1021 acaagagaag ctgacttatc atagactgta tttgctttat taataagagc aatataatca
     1081 cctttgtaat aatctataaa acttgctgct gggtacacag ctaatgatgt acctcctata
     1141 atcaataaat cagcattact tatagcagaa attgttttag taatgacatc actgtcaaga
     1201 gcttcctcat aaagtactac atctggtttt actatagaac cacagttgtc acagtaagga
     1261 atattacctc ccagatttaa catagattct aagtcaaaaa acttaccaca tttagtacaa
     1321 taatttctat gtacagaacc atgaagttct agcacatttt tacttccagc catttgatga
     1381 agtccatcaa tattttgagt tatgacagct ttaagcttac ccatttcttc gagttttgct
     1441 aaagctatat gagcattatt gggttttgca ttaggatata ttaatttatc cttataaaat
     1501 tcaaaaaatt cttctggata tctaacaaaa aaagtatgtg aaactaattg ttcagcagta
     1561 aattgtttat tgagtttttg gctaaataat ccagtagaac ttctaaagtc tggaatatta
     1621 gattcagttg aaacacctgc tccaccaaaa aatacaatat tattgtgatt agctattaag
     1681 tcttttaagc tatttgcatc catagttaca cctccaaatt attatatata aattgtacta
     1741 catattaggt cgtttatgtt ttattaattt aaataaatat atagtttaat aagttaaata
     1801 aagggaatat aaataaaatg tatacagagt ataaatagac aatatttact aaaataacaa
     1861 gttgtcgtat aattatatta aaattgtttt agtgtataag aagggcgggg gaagtacgtg
     1921 aagaaaaaag cagctttagc aacattggct atgttaccct taggtgtggt aaatgcacat
     1981 gctgatggag atataggtat agtgactata aattatttaa atgtaagaaa tgaaccaact
     2041 gccgaaagta gcatagcctt tgttgctaaa aaagatgata aagttttgat taaagactct
     2101 tctaatggat ggtataaaat aaaagctgaa tctggacaag aaggttgggc ttcatcaaaa
     2161 tatatagcaa aatcaaatag tgactcttta agaacgtcta caaataaaga aaaacaagtt
     2221 atttcaaata gtttaaatat gagaaatgga gcaggtacaa gttacagagt aataactgta
     2281 ctaaaaaaag gtcaaaaagt agaagtaata tcagagagta atggatggtc taaaatcaaa
     2341 tatgatggaa gactaggata tgtatctagc tcttatttag gagacgtttc aaactcaact
     2401 aataaatcaa aaactaaaca agtgaataca acttcactaa atgtaaggag tggaccaaat
     2461 acaagttatg gtttgttagg caagttgcca aaaggaagta aagtagaagt aatatcagaa
     2521 agtaatggat ggtccaaaat aaagtataat ggaaaagatg cctatgtatc tagtatgtac
     2581 ttatcggatg taagtcaaag taattcagac aattctagtc aaagcaatga taaaaagaat
     2641 actgataagg ttgtaaatac agcttcttta aatgtaagaa gtggaccagg ttctacatat
     2701 agtaagttgg gaaaagttta taaaggaagt aaagtaactg tactatcaga aagtagtgga
     2761 tgggctaaga ttaattttaa caataaagaa gcatttgtag taggcaatta tttatctacc
     2821 tcagcagata cttcaaataa taactcaaat agtaactctg ataacagttc taatagtaat
     2881 ggtaataatt cttcatcatc aggccaagta aatggtatgt caggtataag tggggctaaa
     2941 attgattata aatctttgag ttatacttta gaatctcata taagtaaaca agttgagaaa
     3001 gcagcatcag gaggaaatgt gatagctcca agtaatagga aaagtactcc aagtcctgag
     3061 tttagtactt tttcagctca aagaacaagc tcatttgtaa atgcaagttc aagtgatata
     3121 gaatattatt taaatcctaa gaattttaca aatactacta aaggtatgat gcagttttta
     3181 aagattaaca gttatagaga tggtatttca gaatcaagtc ttaactcgta tcttaatggt
     3241 ttatcttcta gtgtatttaa aaaccaggga gcagccttta taaatgctgc caagaagtat
     3301 aatatagatg ttgtatatct agtatctcat gctatgtggg aaactgctta tggtaagtct
     3361 acacttgccc aaggtcaaac attaacatct tataagggac aacctcttag taagccagtt
     3421 aaagtataca atttttttgg tataggagca atagataaaa gtgctaatgt ttcaggtgca
     3481 gaagcagcgt attccaatgg ctggactagt gtagaagcta caatagatgg ctctgcaaag
     3541 tggatatcac aaaattacgt aaatagttct aagtacaatc agaatactat ctacaagatg
     3601 aagtggaatt atgattatac atggcatcag tatgcaactg atgtaaactg ggctaatgga
     3661 atttctggaa ttatggaaaa tttaattggt ctttatggag gaggaagtag tttggttttt
     3721 gaagttcctc aatataagta atacataaat aaaaaatata gactatgggt agttttcatt
     3781 ttatttaaat tatctatagt ctttttgtat ataatttata atctgtaaat ttaatgttaa
     3841 caaaactttt atgttataat attaattcta agtaaatata agtgagtaat aggtagaaat
     3901 agataaaaag tggtaataat atatataaag ataattttga gaaaggagga tatatatgga
     3961 aagtattaag gaatatttgg ataagttaga aattaataat agtggtcttg gaaagcaact
     4021 taaagaagtt tatataaata gagtagttta ttttaaggaa gataagatag tttattttta
     4081 tctaacttct aaagatattg tttctcatga acttttagat aaatttaaag aagaattgat
     4141 gtataaactc gattatttta aagatatgag gatgaagata agatttactg gtcttgaaag
     4201 aaaatccaat aaagatgtta taaaaaaata ttggaataat atattgtata tattaaagta
     4261 tctttgtcca tctattgcag ggtggtataa acaagtagaa tttctttgtt tagaagaaga
     4321 attaaaaata aaacttccaa aaggtatatt ttatgagcga ttaatgaaaa aaaatgtaac
     4381 ttatgtactt aaaaccgtgc ttagcgaaga gttgggatta gatttaaata taacaataga
     4441 aaaagctgtt gatgaaaagg taaataaaga aagactcata agaatcaatg atagagaaat
     4501 ggaagaaaaa attagggctt tagaaattgg aaaagttaat aactgtgaag aaaatgaaga
     4561 ggaaagctat gtcattaagt ctgatgtaga tgaaaattta atctatggag ataatgcaaa
     4621 tgctatgatt gaaaatatag ttgaattaaa tgcatcttct ggaacagttg cagttgttgg
     4681 agatatattt gatgttgaca ctaaagaact taaaaacgga aaaatactta tgatagcttc
     4741 aattacagat tatactagct caataagctg taaacttttt ttaacagata caaataagga
     4801 tggagtactt gaaaatgtta aaaaaggagc atacttaaaa atcaagggtg acatagttta
     4861 tgatacatat caaagagaaa ttagtatgat gattagtggg ataagaaaag aaactagaat
     4921 agagagagaa gataaatcag aggaaaaaag agtagaactc catgcacata cacaaatgtc
     4981 ttctatggat gctatttgtt ctgttaaaaa gcttgtagag cgtgcagcca aatggggtca
     5041 ttctgctatt gctataacag accatggtgt tgtacaagga tttccagatg ctatgaatgc
     5101 aggtaagtca aataatataa aaatacttta tggagtagaa gggtacctgg ttgaagatga
     5161 ttctctcatt atagaggatg caaatgataa agaactttcg cagacatttg ttgtatttga
     5221 catagagaca actggctttt ctaatacaaa tgataaaata actgagattg gtgctgtaaa
     5281 aatagaaaac tttaagattg tagatagatt tagtgagtta ataaatcctg aaaaagatat
     5341 ttcttataag atacaggaat tgacaggaat aactaatgaa ttaattaaag acaagccaac
     5401 cattgaagaa gttcttccta agtttatgga atttgttgga gatagtgtat tagtagcaca
     5461 taatgcagag tttgatactg gatttatatc gcaaaaatgt agggaacaag gcttgttata
     5521 taataacaaa aaagtagata cacttatgtt agcaagagta atgttgccaa acttaaaaag
     5581 acatagatta aatgtcgttg caaaagaact tggtattcct cttttaaatc accatagagc
     5641 agtggatgat gctgaagcta cagcacttat atttaataag tttttgcaga tgcttacaga
     5701 aaaaggtgca aaaactctaa gtgatgtaaa caatatactt ggaaagattg attacacaaa
     5761 gcttagcaca aatcatataa ccttgatagc aaaaaattct ataggtataa aaaatttata
     5821 taaaataatt tcagatgcac atgtaaatca tttttttaga gcaccaagaa tccttagaag
     5881 tgtattaaat gaatataaag aaggattaat aattggttcg gcttgtgaag ctggggtggt
     5941 ttttcaagct gtaaagaaaa acgtgtctga tgaggaaatg aaaaaaataa tagatttata
     6001 cgattatatt gaagtaatgc ctatagataa taatagattt atgattgata aaggagaagt
     6061 aaaggacgaa gaagagttaa gggagttgaa taggaaacta attgatacag ccaaaaaatt
     6121 tgacaaaatt ccagtagcta ctggggatgt tcatttttta gacaaacatg aagctgtctt
     6181 aagaaaagtt cttaaatatt ctcaaggttt taaagtagat gaagaagaaa cttatttaca
     6241 ttttagaaca acagatgaaa tgttggaaga gtttagttat cttggagaag aattggcata
     6301 tgaagttgta gttgaaaata gtaatctgat tgccgatatg gttgaagata taaaacctat
     6361 accagatgat acttatcctc caattataga gggctcagat gtggagttaa gagaaatgtg
     6421 ttacaaaaaa gccaaacgaa tctatggaga cccaatacca gaaatagttc aaaaaagact
     6481 ggatagagaa ttaaattcaa taataagcaa tgggtatgca gttatgtata ttatagctca
     6541 aaaactggtt gcaaaatctc tttcagatgg atatttagtt ggttcaagag gctcagttgg
     6601 ctcatctttt gcagctacta tgagtgatat aacagaagtt aatcctcttc cagcacatta
     6661 tatttgtgaa aacgaagact gtaaatattc attcttttat gaaataggtg aatggggctc
     6721 aggtgttgat ttaccagaca aggattgtcc aaagtgtgga agaaaacttg ctaaaaacgg
     6781 tcatgatatt ccatttgagg ttttcttagg ttttgaaggt gataaggaac cagatataga
     6841 cttaaatttc tcaggaaatt atcagccaac aattcataaa tacacagaag aactatttgg
     6901 agaggggtat gtatataggg ctgggactat aggtactgta gcagaaaaaa cagcttttgg
     6961 atacgcaaga aaatatgtgg aagaaaataa tataagtgta ccaaatgcag aagtacttag
     7021 gctttcaaat ggatgtactg gagtaaaaag aacctcagga caacatcctg gtggagttat
     7081 ggttatacca gactataaag atgtatacga ttttacacct atacagtatc cagcaaatga
     7141 tacttcttgt ggtgttataa ctactcactt tgattatcat tcaataagtg gtagaatact
     7201 taaacttgat atacttggac acgacggtcc taccattata aggatgcttg aagatatgac
     7261 tggaataaat ataacagaaa ttcctttaga tgataaagag actatgtctc tatttacatc
     7321 tacagaagca ttaggtgtta caccagaaga aataaactgt cctataggtt gccttgctgt
     7381 accagagttt ggaactaagt ttgtacgaca aatgttactt gacacaaaac ctactacatt
     7441 tgcagaactt gtacgtatat ctggtctctc tcatgggaca gatgtttggg taaataatgc
     7501 acaagactta gttagagaag atatagttgg attaaaagat gttatatcta cacgagatga
     7561 cataatgaac tatttaatat ttaaaggatt acctcccaaa atgagcttta caataatgga
     7621 gagtgtaaga aaaggtaaag gtttaaaacc agaacacatt gaagaaatga aaaaaaataa
     7681 tgttccagaa tggtatatag gctcatgtaa aaagattaag tacatgttcc caaaagcaca
     7741 tgctgttgct tatgtaatga catcatttag aattgcatat tgtaaagttc actatccaga
     7801 agcattttat gcgacttatt ttacgacaaa agttgaggat tttgatgcag atttaattgt
     7861 taaaggatta gatgctataa aatcaaaaat aaatgaaatt gagtcactgg gaaatgatgc
     7921 aaccacaaaa gaaaaaggta tgttgaccgt tttagaagtt gctcttgaaa tgtatgctag
     7981 aggaataaaa ttattaccag ttgatatata taaatcagat gcaactgagt ttatagttgt
     8041 aggagaaaaa acattacttc caccaatggc agcaatacaa ggtcttggag gaaatgcggc
     8101 tataaacatc caaaatgaaa gaaaaaatgg tgaatttata tcaaaagaag atttgagaaa
     8161 gagaacaaaa atatcaaaaa cggtaataga aactcttact aatcatggtt cacttgagaa
     8221 tatgagtgag agaaatcaat tgtcattgtt ttaagttagc ccaaaaagtt gcaaataaaa
     8281 gaattttatg atatcatagt atagaaaata gtatactttg cagaaactaa gagtgagaca
     8341 gctgttctca ctcttttatt ttaaatatat tattaaaaag ctggtttatt tttattaaat
     8401 atattttatt taagattata taaactggtc atttacaatt gaataggagg aactttaatg
     8461 aaaaaaaatt tagaagctac aattgaggag attgtaacta aaataacgga tgaacatgga
     8521 tttgaaatgg ttgatgttga atatgtaaaa gaagcaggag aatactactt aagagtatac
     8581 atagataaag aagaaggaat atctttaaat gaatgtgaat tagttagtag agaattaagt
     8641 ccaatactag atgaaaaaga ccctataaaa gaaaattact ttttagaggt atcttctcca
     8701 gggcttgata gagcattaaa aaaagacaga gattttgtta gatatcaagg tagagatgta
     8761 gatttaaaat tatataaacc attaaatgga tgtaagcaat ttgaaggtga acttgtagga
     8821 ttaacagaag ataataatat aaaaatcatt gcaaatggta aggaaatgga atttaataga
     8881 aaagatgttg ctattgtcag attagcaata aagttttagt ggtatattaa atagggagga
     8941 tataaaaaat gaatcatgaa tttatggaag cattagatga attagtagtt gacagaggta
     9001 tagataaaga aatacttata gatactatag aacaagctct tttaactgca tacaagaaaa
     9061 actttggttc agcacaaaat gttagagttg aatttgatag agacaaggga gatataaaag
     9121 tattttctca aagagttgtt gttgatgaat cagatttata tgatactttc ttagaaatag
     9181 agttagcaga tgctagagag ataaatccta attatgaatt aggtgatatt atagaacatg
     9241 aagttacacc taaaaacttt ggaagaattg cagctcaaac agcaaagcaa gtagttgttc
     9301 aaagaataag ggaagctgag agagaaatag tgtataatga atttatagaa aaagaaaatg
     9361 aaatagttac aggagaggtt gctagagcaa ataaaaatgt tgtttatgta aatcttggaa
     9421 gaatagaagg tataatgact caaagtgaac aaataccatt tgagaattat aaagcaggtc
     9481 aaaaaataaa attctatata cttgaagtta aaaagactaa caaaggacct caaatagttg
     9541 tatcaagaag tcatccgggt ctagtaaaga gattgtttga agaagaagtt cctgaaatat
     9601 atgaaggaat agttcaaata aaatctgtag caagagaagc agggtcaaga actaaaatgg
     9661 cagtaagatc tatagatgaa aaaatagacc caattggagc ttgtgttgga ccaaaaggac
     9721 ttagagtaaa aaatatagtg gatgaacttg gcgatgagaa aatagatata ataaaatata
     9781 gtgaagaccc agcagagttt atatcagcag cacttagccc atcaaaggtt gttagagtag
     9841 atatagatga agatgaaaag tctgccttag taatcgttcc agattatcag ttatcgcttg
     9901 ctataggtaa agaaggtcaa aatgcaagac tagcagcaaa attgactaac tggaaaatag
     9961 atattaagag cgaatctcaa gcagaacaag aaatgttaaa tcttgaaaat tccaaagaaa
    10021 tagaagctga ggatgttgaa gctgaattta tcgaccaaga agagttagat acttcagagg
    10081 ataattttag tgaagaagag taaggaggga tgattttgaa aaaagtaaaa aaagtgcctc
    10141 aaagaaaatg catagcatgt caagatagag actctaaaaa agagcttata agaatagtta
    10201 aaaataaaga aggaaaaata tttttagacc caacaggaag agccaatgga agaggagcat
    10261 atgtatgtaa aagctctgaa tgtctaaaaa aagctataaa gagtaaagca ttaaataaag
    10321 cttttaaaat agatgttcct gatgaagtct atgataatct acttatggag ttagaagaat
    10381 atgaaaaata ataaagaaaa agtactttcg ttcttagggc tcgcaactcg tgcagggaaa
    10441 atagtgtctg gtgatgattc aactttgctt gaccttaaaa aaggaaaagt aaatttgatt
    10501 ttaatcgcag aagatgcatc aaataatact aaaaaacttt ttaaagataa atcaactttt
    10561 agaaatattc catatctatt tttttcaaca aaagaggaaa taggatttgc aataggaaaa
    10621 tctccaagag cagttgtagg aataaaagat gaaaattttt ctaaaaagat aattgaacta
    10681 atagaaattt aaaataaaat agggggtgat cttgtgtcaa aaacaagagt ataccaaata
    10741 gcagaagaat taaatatatc aaatgaagag cttataaata agttagctga attagatata
    10801 aatgtaactg acaaagattc agtattagaa ggtgaagagt tagaattagc acttgaaatg
    10861 ttaggtgaag atttatctca agaaaatgga aatgttatag aaatagatgg aaagttgaca
    10921 gtacaagtat tagcaacgaa attagataaa tctccatcag aaataattat gaagcttatg
    10981 aaaatgggaa ctatggcaac aataaatcaa gagataagtt ttgagattgc tgctttggct
    11041 gccaaagact atggatttga attaacagta gctgaaagtg atgatacaga agctttagaa
    11101 atagaagctt taatggaaat tgaagaagac aaagaagagg atttaaaacc tagaccacca
    11161 gttgttacag ttatgggaca tgttgaccat ggtaagacat ctttactaga tgcaataaga
    11221 aaaactgatg tgatctcagg tgaagctgga ggaataactc agcatatagg tgcttcagaa
    11281 gttaaaatca atggacacaa aatagtattt ttagatactc caggacatga ggctttcaca
    11341 tcaatgagag caagaggagc acaagttaca gatatagcaa tattagtagt tgcagcagat
    11401 gatggtataa tgcctcaaac agttgaagct ataaaccatg ctaaagcagc aggtgtacct
    11461 ttaatagttg ctattaataa aatagataaa ccaggtgcaa acccagataa ggttaaacag
    11521 gaattagcag accaaggatt attagttgaa gactggggtg gagaagtaat agcagttcca
    11581 gtatcagcta agaaaaaaga aggaatagat acacttttag aaatggtatt attggttgct
    11641 gaaatggaag agttaagagc taatccaaat aaaagagcag taggaactgt tatagaagct
    11701 gagcttgata aaggaagagg gccagttgca acagtacttg ttcaaggtgg tactttaaca
    11761 gtaggtgacc caatcgttgc aggtgttgca tgtggtaaag ttagagcaat gataaatgca
    11821 aaaggtaaga gagtaaaaac agcaggacca tctacagcag tcgagatatt aggtctatca
    11881 gaagttccac aaggaggaga ccaatttgtc gaagttccaa ctgataagat tgctagaagt
    11941 gtagcagcaa gaagacaaca aatagttaga gatgaaatgt taaaatcaac tcaaagatta
    12001 tcactagatg ctctatttag tcaaatgagt gaaggttcta taaaagacct taatatagtt
    12061 ataaaagcag atgtacaagg ttcagtacag gcagttaaac aatctttaga aaaattatca
    12121 aatgaagaag ttcaagttaa agtaatacat ggtggtgttg gagctgtaac agaatcagac
    12181 atattacttg cagcagcttc taacgcaata ataataggat tcaatgtaag accagttcca
    12241 ggtgcagaat ctctaggaga aaaagaaaat gttgatataa gaacatatac tataatatac
    12301 aaggcaatag aagatataca agcggctatg actggtatgt tagaccctga atatgttgat
    12361 gaagaaacag gaaaagctga aataagagaa atatataaaa tatctggtgt tggaacagta
    12421 gctggatgtt atgtgactaa tggtaagata tttagaaatt gtaaagtaag attagttaga
    12481 gatagcataa taatacatga aggtgagctt gcagcactta agagatttaa agatgatgta
    12541 aaagaagtta attctggata cgaatgtggt atgagttttg taaattataa cgacataaaa
    12601 gaaggcgata tagtagaagc ttatataact aaagaagtag aaagaaaatt ataaattcgt
    12661 atcaaagaaa atcagttagt aaagaaggtc gatggtatgg catcatataa tagaacaaga
    12721 aggatagcag aagaaataag aaaagtcgta agtactatgc ttataaatgg agtaaaagac
    12781 cctagaataa catctatggt gagtgtaact gatgtagaag ttacaaatga cctaagatat
    12841 gcttatgtat atgtaagtat tctaggcgga gatgaagaat ccacactaac aggtcttaaa
    12901 agtgctggag gatatataag aagagaagta ggaaaaaata taaaacttag atatattcct
    12961 gagatagtat ttaaacttga tgattcaata gaaaaaggaa tgtatatgga tagccttata
    13021 aaaagagtta atgaaaaaaa tgctcaacaa aatgaggatg aaaattatga tgaataatat
    13081 ggatagtatt ataaactata tttatgaggg tgactatttt atagttacct ctcatatcag
    13141 tccagatgga gacaatattg gttctacact ttcaatgtat tatacattga agaatttagg
    13201 aaaaaatgta tattatgtac ttgatgatga agctccatta aatttaaggt ttttggttga
    13261 aggtgtaaaa atctataagt ctaatgaatt taatatgaag aactattctc taattgcact
    13321 tgattgtggg gataagatga gaatatgtgt gagtgatgaa atcaaaaata atgcatcaaa
    13381 attaatctgt attgaccatc atgctagtaa tgattcatat ggagatttaa attatgtaga
    13441 tattgatgca tcttctacat gtgaattagt ttataattta ttagttagat ttcagcaaat
    13501 aaaagatatt aatattataa atgaagatat agcaacatgt ttatatacag gtcttgtaac
    13561 tgatactgga aacttttcgt attcaaatgt acatgcaagt agctttgaaa tggcaaaaaa
    13621 tttacttgtt ttaggtgcac aaaaaaatac tataatacaa aatatatatc aaagcaattc
    13681 ttctggttat tacaaattat taggagaagc tctaaagggt ttggagatat ttgattaaaa
    13741 aatagttacg cacactcgtg actttagtca tgagttagta gctaaaatag tcagcatata
    13801 gggaaactta tatgtagaga taggata
//
LOCUS       DAMJVI010000095        13550 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.100, whole genome shotgun sequence.
ACCESSION   DAMJVI010000095 DAMJVI010000000
VERSION     DAMJVI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.100"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            325..1755
                     /locus_tag="PC545_002897"
     CDS             325..1755
                     /locus_tag="PC545_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457653.1"
                     /translation="MSSQESKPKGNLISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAVAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIEDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            1858..2961
                     /locus_tag="PC545_002898"
     CDS             1858..2961
                     /locus_tag="PC545_002898"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2457654.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAIEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKGEPSIG"
     gene            2976..3212
                     /locus_tag="PC545_002899"
     CDS             2976..3212
                     /locus_tag="PC545_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2457655.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            3229..4278
                     /gene="vorB"
                     /locus_tag="PC545_002900"
     CDS             3229..4278
                     /gene="vorB"
                     /locus_tag="PC545_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HDF2457656.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVIEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREGDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RITENEQQWEDYQVEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALTSKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            4280..5020
                     /locus_tag="PC545_002901"
     CDS             4280..5020
                     /locus_tag="PC545_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HDF2457657.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSVNGRDIKTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWGV
                     SLEKSIKWMEEEIVPYYALGEFKQRDGE"
     gene            5023..5562
                     /locus_tag="PC545_002902"
     CDS             5023..5562
                     /locus_tag="PC545_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HDF2457658.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENVT
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNVQNEAAFMEGYNNAI"
     gene            5686..7785
                     /locus_tag="PC545_002903"
     CDS             5686..7785
                     /locus_tag="PC545_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HDF2457659.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKETDRVYYVN
                     PKREELFGRKCYKTIQEVPEVVDCVVVCTPRNVVPSVLKDSGELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDDYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPANSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDDSVGFTMVGANIRDEE
                     NEMHETLCKAVSEAREQGLKKPVFAVPTLEGNRYLDYRTRLEDNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIENRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(8052..8567)
                     /locus_tag="PC545_002904"
     CDS             complement(8052..8567)
                     /locus_tag="PC545_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HDF2457660.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YAAPTNPPVTI"
     gene            complement(8659..9870)
                     /locus_tag="PC545_002905"
     CDS             complement(8659..9870)
                     /locus_tag="PC545_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HDF2457661.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIREIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVDFIKEMSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTASASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            10546..11988
                     /locus_tag="PC545_002906"
     CDS             10546..11988
                     /locus_tag="PC545_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HDF2457662.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGLI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPNETKQRLKNVKKIY
                     IIGKELSISKSVETELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSAAQNNGDSSTKGESPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            12030..12383
                     /locus_tag="PC545_002907"
     CDS             12030..12383
                     /locus_tag="PC545_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457663.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFATDIFNIPDIQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTNWILSLIVSLVTYIVLMYIFLNDSAYIYVLLYTAFYMIG
                     HITAKLYYKIKTFKN"
     gene            12574..13395
                     /locus_tag="PC545_002908"
     CDS             12574..13395
                     /locus_tag="PC545_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HDF2457664.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNVPVPSKEYFKGLSKEDAYYKYFNSILETNREFGDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDVIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFVNIEKVKSNNIA"
BASE COUNT         5172 a         1577 c         2433 g         4368 t
ORIGIN      
        1 ttgaataaaa ttataaaaaa ttaaaatcac tagaaatatg ataaaataat ttaaaatacg
       61 catttcaatg acatttcaaa acaataaaat aataaaaaac aaacctaaat taagtctaaa
      121 tagcttttta gttaatttta gtagtaaaaa ataaaactta aaaagtgttt gacaattatg
      181 aattttgaga atataattaa attaaaatta tacacatata aaaatatact cctgaatcaa
      241 gaatgagata tttaagattc aaaatatata ttattatacg tgaataataa aaaagtaaga
      301 aaattttata agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa aggaaatctt
      361 atatcaaatt ttagagatga tatgagagac cctaagaaaa gagcaatata cacatattca
      421 agttctataa acttagcgta caattttata ggttactttg tatcaactgt tacaaatatt
      481 tttttaactg atgtggcaaa acttggtgta attatggcag gtggtatgca aattatacaa
      541 tctttcataa aaattgctgt agctcctata acaggtacag tatttgacaa acaaccattc
      601 aaaaaaggaa agtattatcc atggcttaga tatgcaccag cagcgttagc attaacatat
      661 ggaatatttt tcatagttcc attaatgaaa ataagtccag cattactttt acctttgatg
      721 acagctttat tattaatagc atcttgtgct cagcaagtag ttttcactat aatttatgct
      781 atttaccctg tagttgctaa gacacctaga gaccgagtta taggctcaac tactacgaat
      841 atatttaaag aagttggtaa attcttagta ggtttttcat atccgttatt attagtttta
      901 tttgcagatg tattaggtgg agaaggaatg ggttatttag gtacctactt attctttgca
      961 gcaggatgcc ttttagtatt ttggttttct tcaacagaga taataaaatc tggagcagaa
     1021 gaagaagttc ttcaaaaaga agagaagcca aagattaaag caagtgaaat ggcaaaagct
     1081 ttattcacta atgtaccact tatgatggca ttttgtttag aatttcttat atgtataaga
     1141 tctataggat taggaccttt ggctccatat tattttaaat atgtaataga agatgaacgt
     1201 ggattagcag tatttttatc aatcatgcct ttggtaagtg tagcattcat gtttttcgca
     1261 ccagtgttta taaaaatctg tagagagcaa aaactagctt caataatttc tttttctata
     1321 tgtgcactat gccacttatt ggttgcatgg actccttggg ggaaaactac tttaggagta
     1381 actatgttgc tggcaattgg tggaggtttt tcaaatgtag tatcaataat aaatcttaat
     1441 ttctttgccg gttcttgtga ttatgggcat tggaaatcag gaaaagactt acctggctta
     1501 agtatgtcac tatatccagt tgcaatacaa gtaggagtat tacttgcaac tactataaga
     1561 acagttttaa tgaactcaat gggatatcaa gctgatatgg tagtcacaga agcagttaaa
     1621 tcaggtttta taaatatgat ttcttattct atggcaatac ctttaataat agctgttgtt
     1681 atagcaatac tatatccagt aagtgacaag aaattaaacc aaataagaga agagttaaat
     1741 caaagaaatg cttagttaaa agaaagttaa gtagatggtt aatttaagct attaaagtaa
     1801 gtagacgcaa tagcgtttat ataaatgaaa atacatatac agagggggct tttaaaaatg
     1861 atagaaaaat tattgagaaa taccgtaaaa gaattacatc aatatgttcc aggtgaacca
     1921 atagaaaaag ttaaggaaaa atatggtgta aaagaaataa taaaattagc ttcaaatgaa
     1981 aatcctctag gaccatcacc aaaagcaata gaagctatga ttgaaatgtt aaaacaaggt
     2041 caactttatc cagagccaga agctaacgaa cttagaagaa aattagctga aaagctagat
     2101 ttaaaaccag aaaattttat tgttgctaat ggtgcagata acgttatcac tctaataggt
     2161 gaggctttta taaatagagg agatgaagtt atatattgta acccaacatt tccatcatat
     2221 agaactgcaa ctataaaaaa tgaagggata ccagtagaag tacctttaac agaagattat
     2281 aagtatgatt tacaaggtat attagataag attacagata aaacaaaatt aatatgtgta
     2341 tgtaacccta ataatccaac aggaacaatt gtggatgata aagagttaga agaattttta
     2401 aagaaagtcc ctgaaaatat tataactata ttggatgaag catatataga atttttaaca
     2461 gttcctaatt atgtggatgg acttaagtat gtaagggaaa attataacgt tatagtaaca
     2521 agaactttct ctaaaatata tggtcttgca gggcttagag taggatatgg tattgctaaa
     2581 gatgagataa taagaacttt atttacagta aaagagcctt tctctgcaaa tcgtgttgct
     2641 ataagtggag caacagcagc acttgatgat gaagagttta taaaagaaac atatgaatta
     2701 aatagagttg gaatggctta ctttaaagaa gagtttacaa aaatgggatt tgatgttgta
     2761 gattcacaat caaatttcct ttatgtagat atgaaaacag atatacctaa gttatttgaa
     2821 gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta cacatgcacg tgtaagtatt
     2881 ggaactatgg aagaaaataa gaaatttgta gcagttttaa aggaaatatt aaatattaaa
     2941 ggagagccaa gtattggata atagatagaa atattatgaa acggggtgta gatatgaact
     3001 taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg cgttatatct tgtaaaaaag
     3061 gagcattaaa aatatcaagt aagataaaca aagaaggtta tgctcatgta gaagttgatg
     3121 agagtaaatg catactttgt ggaatttgct atcaggtgtg tccagataat gtttttgaaa
     3181 taattaaaga agaagcatgt caagaggctt agaaggagtt gggataatat gaaaaagatg
     3241 tggaagggga accacgccat tgcagaagca gccctaagag gaggttgtga attttatgca
     3301 ggatatccaa ttacaccaca aacagaggta attgaatttt tatcacatag aatgtctgaa
     3361 cttggaagaa cttttataca gtccgaaaat gagatggctg ctatatatat ggtatatgga
     3421 gcatatgcaa gtggaatgag gtctatgacg agttcttcag gaccaggaat atctttaaaa
     3481 caagaaggga tttcttactt atgtgcaaat cattaccctt gtgtaatcgt aaatgtacag
     3541 agatgggggc ctggtcttgg ctcattggac tcagcacaga cagactatct aagagatact
     3601 agagggggag gaaatggaga ttatcatttg atagtttatg caccaaacag tattcaagag
     3661 actgtagatt tgatgtataa ctcatttgat gtagcggaga agtacagagt gcctgtcgaa
     3721 attttgacag aagcagcttt aggtcaaatg atggaaccag tagaatttcc agagtttaag
     3781 aagagagaag gagatttagg ctggacttat gatggaagta atagagacca tgcaaaggtt
     3841 gcagataacc aaaagccaac tttttgtatg gagaaacgta tgagaataac tgaaaatgaa
     3901 caacaatggg aagattatca agtagaagat gcagaatatg tgtttgtagc gttcggtata
     3961 ccatcaagaa caactatgaa tgctgttaga agacttagag aacaaggtga aaaagttgga
     4021 ataataagac caataacagt atggcctttc ccatataaag catttgaaaa ggtaagtaaa
     4081 gatgtaaaag gatttatctc agtagaatca acagacacag gtcaattagt agaagatgtt
     4141 gctttaacct caaagaaagt atgtaaagaa aatgtacctg tgtatggttt atttagtgga
     4201 aatcatatac caaaaacatt gcaagtaatg gacacttata gcaaaataaa atctggcgaa
     4261 atcaaggagg tgttctaata tggctacaga aaaattaaca cctagcgttg tttcaatacc
     4321 aaacaagttt tgcccaggtt gtggtcatgg aatagtaaat cgtataatag cagaggtaat
     4381 agaagaacat ggatatgaaa aaaatcatgt attaacatta ggagttggtt gtgtctgcaa
     4441 tatgaatttt agctggaatg gtgataagat gcaaactgca catggtagag cttcatctac
     4501 agctatagga gttaaagtag cacttccaga tacattagta atgacttacc aaggtgatgg
     4561 agatgcttat gttataggtc tttctgaaac tttaaataca gcatatagaa atcataatgt
     4621 gactgtattt gtaataaaca ataataattt tgcaatgact ggtggacaaa tgtcatggac
     4681 cactatgccg ggtcaagtca caacaacaag tgtaaatgga agagatataa agactactgg
     4741 tagtccactg aaagttcctg agatggtggc aaacttcttt gatgtagcat atgtagcaag
     4801 aggttcagtt catagcccaa aggaaataat taatctaaaa aaatatataa aaaatgcaat
     4861 agaagctcaa ttaaatggag agggatattc cttagttgaa atattagcac cttgtccaac
     4921 taactggggt gtatcactag agaaatctat caaatggatg gaagaagaaa tagtgccata
     4981 ttatgctctt ggcgaattta agcaaaggga tggtgaatag ttatggaaaa agagctagta
     5041 tttgcagggt ttggtggtca aggagtttta actcttggtc tgattgtagc tgagtcagca
     5101 cttgaattgg ggaaacaagt aacttggatg cctgcatatg gtccaactat gagaggtgga
     5161 aaagcctata gtgtagtaaa gttttctgat gagagtatag gtgggcctga tatggaagaa
     5221 atagatgttt tagtagcaat gaataaacca tctttagatt atataaattt agttaaagaa
     5281 ggtggaacag tagttattaa tacatctgca atagatgaga atgtaacatt aagagaagat
     5341 ataagtgtag ttaaaataaa ttgtcaggaa ttagctcaaa aagtaaacaa tccaaaagca
     5401 gcaaatatag tagtacttgg agcattaatt gctaaaacag ggttatttga aaaagaatta
     5461 gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt taatgttcaa
     5521 aatgaagcag cattcatgga aggctataat aacgccattt gataaattac ccttatctta
     5581 agtgatttat ctcaagattt aataatcttt agtgccaaga gaaacttcat cttgagataa
     5641 aattcactta aaatcacaac tataaacata taggagcgtg aatttatgag tttggaaaaa
     5701 cttattagac caaaaagtat tgctatagta ggagtgactg ataaacttgg ttttggaaga
     5761 agtgcagcac ttagtatagt aaaaagtaag gaaacagata gagtatatta tgttaatccc
     5821 aaaagagaag aattgtttgg gagaaaatgt tataagacga tacaagaagt tccagaagta
     5881 gtagactgtg tagtagtatg tacacctaga aatgtagttc catctgtatt aaaagattct
     5941 ggagagttag gaacaaaggc agcagtggta tatgctagtg gatttgcaga ggaaggtaca
     6001 gaggaaggta cagatttaga aaatcaactt atagaaatat caaatacata cgatatgaaa
     6061 atattgggtc caaactgcat ggggttgcta aattgtatag ataaggttaa tctatgggca
     6121 ggaggttcta aatgggattt agatactaaa aaacctggta taggaattgt aggtcaaagt
     6181 ggatttataa ctgctgaaat tgtttcaagt gattatttta atatatctta tggtttttca
     6241 acaggaaatg gaaatatagt tacactagaa gatgcagtag atttccttgt agatgatgat
     6301 tatgcatcag taatagcaat ttaccttgaa ggtttaaaaa atcctcaaaa atttatagat
     6361 gctttaaaaa gggcagcaca aaaaagaaag ccagtaataa tattgaaatc aggaaggtca
     6421 gaaaaaggtg ctatttctgc tgcttctcat acaggaaatt tagctggttc gagtaaagct
     6481 tttgaaagta tttttgaaaa atatggagta atttctgtgg aaaatttaga acagtttatg
     6541 tgtttagcac aagcttttag tgttttagat ggtaatttac ctgcaaattc taattttgca
     6601 gcaataaact tctcaggagg agaaaatact atttgtgcag atttagcaga agaaaatggt
     6661 gtagaacttg cagaaatatc ttctgaaact aaagaagaaa tgaaaaaata tcttccagga
     6721 tttgcaacac ctaaaaatcc actagatgct acaacagcat tattccatga aaaggatatg
     6781 atagtaggtt tattacatac ctttgaaaag gatgatagtg ttggatttac tatggtaggt
     6841 gcaaatataa gagatgaaga aaatgaaatg catgaaaccc tttgtaaagc tgtaagtgaa
     6901 gctagagaac aaggattgaa aaaacctgtg tttgcagttc ctacacttga aggaaataga
     6961 tatttagatt ataggactag attagaagat aatcaagtgc ctataatgtc atcagttgga
     7021 actacattta cttgttttaa taaaatggct aagtttatag attatgatta ttcaaaaaga
     7081 actttagaat ttaaagctgt taagaaaaga gagtcaaata atgtagtagc attatcagaa
     7141 ttagatagta agattgagat gaaaaaatat ggagtaccag ttccaggaca aggtaatgca
     7201 aaaagtattg atgaattaga tgaaatctta aaatatatta agtatcctgt tgttttaaaa
     7261 ataaattctt ctgaaatatt gcacaaatct gatgttggag gagttaaaat aggaattgag
     7321 aatagagatg aggcagtaga tgcatataat gagattttaa ctaatgtaaa aaaagcaaaa
     7381 ccagatgcta atattgatgg tatattagtt caagaaatgg tagaaagtgg tatagaaatt
     7441 attattggta taactaatga tgaccaattt ggaccaatgt tattagtcgg tttaggagga
     7501 gtatttgtag aggtatttaa agatactaca ttatatccat tacctataaa tcatgatgaa
     7561 gctattatga tgcttaaaaa actaaaatca tttaagcttt taaatggcta tagaggaagt
     7621 gagccttgtg atattgatgc tcttgcagat atgatggtca agttaggaaa atatgcttat
     7681 gaaaataagg atgaagtcaa ggaaatagac ttaaatccag tgtttgtata tcctaaagga
     7741 aaaggcgtat gtgcagtaga tgccttaata gttaaatata aatagtttat aagttttggt
     7801 attcaaccat aattaaagca ccgactgaaa atcacatttt gtaagttaga tagtgtgatt
     7861 ttcagtcacc ccaaatttaa tttaaaaata ttttaaaatg aaagtatagt tttaagttta
     7921 attgtgaaat agcactgtat aaacaataaa atactacgta taaaacgaat aataaaaggt
     7981 gttttaatga taagaatcaa tcttgatttt cattaaaaca ccttctttat tatattaaaa
     8041 atttttaaac gttatattgt cacaggtggg tttgttggag ctgcatatgt acgattagca
     8101 aattcttggt caacaaagaa tagaccaccc tctccagcaa gtttaacacg ttttatattg
     8161 tcttcacaat ttgtttcttc ttctgcttgt tcagatataa accaaagtaa gaattgagaa
     8221 gttctaaagt ctgattcaga gtttgctaca gctgctaagt tgtttattaa agctgtaacc
     8281 tttttttcat gagctaacgt acgctcaaga atatccattg cagaagtaaa gtctgaatct
     8341 ggcattggga tagcatcaag ctgaaccttt cctcctacat tatgtaagta tttgtaaaaa
     8401 aacatagcgt ggtctgtctc ttctttatat tgaacataaa accaattaga aaatccttta
     8461 agtccttctg cttcaaggta agaagacata gaaagataaa gatacgcaga atataattca
     8521 tggttaatct gctcattaag taatttttcc attttagctg aaatcatttt tatacctcca
     8581 aattttaaat gctcttataa aatataataa tattaaatgg aaaaatattc aataaaagtg
     8641 tttaaatatt ttacatattt aataaaaatt tatactacca ttaaaaaaat tataacttac
     8701 aatggctata gagccaatta atggtgcatt tgctccaaaa tttgaatgcc ttacacttat
     8761 ttttttatat ttagaaaaac ttgctgaagc agtcaatttt tttaataatg tattttctat
     8821 aaatgtacca ggtatattaa agtcatagcc tattattata gaactacaat caatcaatgt
     8881 aagtgtatta actaatgcat atgatacgta tgtacaaaat tcatcaagca aaaacatggc
     8941 aagactatct ttattatttg cctcatctat taacttgact aaagaaaaat ctttacagtt
     9001 aattaatggg gagttaggat aaatatggct caatgattca attcttttaa ttagattact
     9061 gacacttgta taataatcta aacatccatt attaccacaa ctgcacaatg gtccactaaa
     9121 attaattgat gtatgtccta tttctccact ttgtcctagg ttgccagtat gaagtttatt
     9181 ttctagaact agaccagctc caataccatt cattatgtgt aggtatatat aatttgatat
     9241 atttttacct aaaccataca ttttttctgc taatgcaccc gaattagcat catttatcaa
     9301 aaatattggc aaatcagaca tttcttttat aaaatctact atatttaggt ttgatacatt
     9361 tccaaaatct gggggattta gtatggtacc atttatatca tctactggac ctattgaaga
     9421 aataccaatt ccaattatat ctctcttgtt gattttcatt attttagtaa acattctttt
     9481 tattatatct tttaaaacat catttgatag acatttgggg taagtttcag aaagttgttt
     9541 tacaatatta ccttttaaat ctgaaataat taccttacat atatttcttc ttattaggat
     9601 tccacaaata caaggcgata tattagaaat atctaataaa ataggtttac gacctgatga
     9661 atgtgcattt aaagttgatg actctatttc cctaatcata ttttcattaa taagttcagt
     9721 aactagattt gataaagtca ttttagtaag tcctgtagac ttagctaaat caactctgga
     9781 cataggtgta tttgtagaaa gtaatttcaa tattattaac ttatttttaa tttttacatc
     9841 tttgatgtta gaaccttgtt tgttactcat cataaatctc cttaaataaa gttaagtgtt
     9901 ttatttattt tcaagtatac tatcacctta taatagagta acattttttt acttgaaaat
     9961 atcattaatt agaatatatc acattatata tcataaaata tataaaaaaa tgttagaatt
    10021 aaattaaaag tgaaaagtag attgatatat aaatagtaaa tacttaatta aaaaaagata
    10081 atgatagcaa taattattgt taccaagaat ctactacttc aagtgttacc ttaggtattt
    10141 aagttgatag tggtttgcac gtaataaact tgatgtaata tatattctta ataatatcga
    10201 taaaagattt acttaaaaat aatatagatt atgtaaacat ataatctatt tattgtatac
    10261 aataaaattt cattttgtaa aacagtgaat taaatatgta atatagatat cgatttttat
    10321 tacaaattgt tactaaatat aggtacatta gaacaaaaat ctagtaaatt gtaactaaaa
    10381 tgtaaatttt tcctttaatc tatacaatat tatggaaact ttttgtaatt tatgatataa
    10441 taacaaatat ataattatta aattataata aatgactata gcgtagatgt aatactatgt
    10501 tatagtcatt tttgaattaa acttgatata tcaaggggga agaatatgag aacaattaga
    10561 agagttttag cattaggttt aacattagct atatttctaa taaatgtacc taatgtagat
    10621 gcattaacat cagatacaat taaaggtaac aatatatatg agacggctgg attaatagca
    10681 gataaaaaga gttatgatac agcaattatg gtaaatatgg ataattctat agcagatggt
    10741 ctttcagcaa gtggtcttgc aggtgctgtt gatgctccaa ttttgcttgc acaaaaaaat
    10801 aagataccaa atgaaacaaa acaaagatta aaaaatgtta aaaagatata cataataggt
    10861 aaagaattat caataagtaa gtcagtagaa actgaactaa aaaacacagg ggcacaggta
    10921 actagattgg gtggagatga cagaataaag actagttata gtgttgctaa agaggtaaat
    10981 ggtatcaaaa aagttgatga agtgatatta actaatgcat ataaaggaga agcagataca
    11041 ataagtgctg cacctgtatc agtaagagat atagctccta tagtacttac agatggaaag
    11101 agtgtacctt tttcaacaag caatgtaaaa acatatgcag taggtgggag tatatcaatg
    11161 agcactagtt tagttaataa aactaatgct aaaagacttg gtggttcaga tagatatgat
    11221 acaaataaga aagttataaa agagttttat ccagatgcat cagaatttta tttgagtgat
    11281 ggatatgatt tagtaaatgc acttacaggt tctacaattg ctaaggaaaa tccaattgta
    11341 ttggtgtcag aaagtagtga taagtctata ttagcaggag cagataagat tactagatta
    11401 ggttcaataa gtgacagtgt gtataataag tgtgtttctg ctgcacaaaa taatggcgat
    11461 tcgtctacaa aaggtgagtc acctatgaaa aatgagacaa gcatattagg tcagccaaca
    11521 gctagtttag aagcgtgctt aaaatgggca aaatctaaaa aagctaatga tttatttata
    11581 gagttaatac caatattata tgatactgct gttcaggaag gtgttaatcc tgttttggca
    11641 gtagctcaat ctgcaaaaga aactggtttt tgtaattttg gtggagtatt agatgcatca
    11701 tttaagaatc cttgtggact taaaacttct gtaggaggtt ctgatactga taagaatgcc
    11761 cattcaagat ttgatacttg ggaagaagga atattagctc aaattcaaca tttatgctta
    11821 tatgcaggac aagatggtta cccactttca aatccagtag accctagaca tgaaaaatct
    11881 ttatttggta aggctaaaac agttgaaagt ttatctaata attgggctgg aggtcaatat
    11941 ggacaagatt tagtaagaat gatgggagaa attgaggcaa caaaataatt gaagattatc
    12001 aaatatccaa taaataagga ttgattatta tgaaaaagag taatattttt agtatcatat
    12061 ttcctacagt cataatggtg ctaatgtttt taacttttgc tacagatata tttaatattc
    12121 cagatattca atctaaaagt atttttgttg tgggaatggt tttgattttt ccaatttctt
    12181 ttttgataca agggataatt tgtgtattaa ataagactaa ttggatttta tctcttattg
    12241 tgtctttagt aacttatata gtattaatgt atatattttt aaatgattct gcatatatat
    12301 atgttttact ttatacagct ttttatatga ttggacatat aacagctaaa ctctattaca
    12361 aaattaagac ttttaaaaat taaattgaag tatataaaag taacattagg agaagtatcc
    12421 ttaatgattt tctaaaatca gaaataggtg cttctctttt ttgtaaaaat aaaagtatgt
    12481 aaataaaatt taaccattat aattttcttt taacaaatgt atacaataaa cttgttgtat
    12541 aattaagata gataaaataa agggagggat aatatgaatt taatagataa tcatattcat
    12601 actaattttt caagtgatgg aaaggattcc atggaagata caataaaaaa agctataagt
    12661 attggtgtta gatatttgac ttttacagac catttagaac atgatgaaga tagaggattt
    12721 tgcataaatt ataatgacta tgttccagta tttaataagt ttaaagaaaa atataaaaag
    12781 gacatagagt tattactagg tgttgaagtg ggttacagaa aacatctaaa aaatgaaata
    12841 gaagaaataa taaattctaa tccttttgat tttgtattgt gctcaactca tacaattgac
    12901 aatgtacctg ttccatcaaa ggagtatttt aaaggtctta gcaaagaaga tgcgtattat
    12961 aaatatttta atagcatact tgaaactaat cgtgaatttg gagattacaa tatatatggt
    13021 catttggatt atatatctag gtatggaata tattcagata atagagttat atacaatgac
    13081 tttaaagatg ttattgatga ggtcttaaag tcaataatca ataatgggag tggtatagag
    13141 ctaaatactt ctggttatag atatggatta aatgctatac atccaaatga agatattcta
    13201 aaaagatata aagaacttgg aggatttata gttactgtag gttctgattc tcatagggta
    13261 gaagatatat gtaaagattt tgatgtggcc tatgatatgc ttaagtatct tgactttaaa
    13321 tatgtatctt tatttaaaga aagagaaact tattttgtaa atatagaaaa agttaaatct
    13381 aacaatatag cttagagtgt gttttgaagt aaattaatgg atagttaaaa agttaatagt
    13441 tataatattg tatcctctag aaaaatacta gaggttttta ttttgctaaa ataactaaat
    13501 agaattaaat ttaaatagta tagtttattt taaattgaat agtttattgt
//
LOCUS       DAMJVI010000096        13396 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.101, whole genome shotgun sequence.
ACCESSION   DAMJVI010000096 DAMJVI010000000
VERSION     DAMJVI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.101"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            191..1504
                     /locus_tag="PC545_002909"
     CDS             191..1504
                     /locus_tag="PC545_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2457665.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIVFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITVGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKTLLKVNSGGSPVNSI
                     LLITVLACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLHRPYKVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            1676..2851
                     /locus_tag="PC545_002910"
     CDS             1676..2851
                     /locus_tag="PC545_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDF2457666.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDSKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFISIKEMFDIDEN
                     AEITIECNPGTLTLEKLKTMKEIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENVDIEMYEYTINYLKSKGYNQYEISNYSKEGYNCEHNILYWECEHYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFREKYNKQIEMLLARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            2954..3973
                     /gene="hrcA"
                     /locus_tag="PC545_002911"
     CDS             2954..3973
                     /gene="hrcA"
                     /locus_tag="PC545_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HDF2457667.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLINQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            4003..4623
                     /gene="grpE"
                     /locus_tag="PC545_002912"
     CDS             4003..4623
                     /gene="grpE"
                     /locus_tag="PC545_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDF2457668.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSSEKT
                     DEKEVDDENVTDINSKLAEKKLQDELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGVEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            4715..6562
                     /gene="dnaK"
                     /locus_tag="PC545_002913"
     CDS             4715..6562
                     /gene="dnaK"
                     /locus_tag="PC545_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDF2457669.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSESEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSGEKEDIEKAIADLKAVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            6773..7927
                     /gene="dnaJ"
                     /locus_tag="PC545_002914"
     CDS             6773..7927
                     /gene="dnaJ"
                     /locus_tag="PC545_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDF2457670.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRARRRGPQRGADIRQSVTISFEEATFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSACNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            8893..9828
                     /locus_tag="PC545_002915"
     CDS             8893..9828
                     /locus_tag="PC545_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDF2457671.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKKRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EGLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            9864..11237
                     /locus_tag="PC545_002916"
     CDS             9864..11237
                     /locus_tag="PC545_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457672.1"
                     /translation="MGKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMCLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGKWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(11425..12192)
                     /locus_tag="PC545_002917"
     CDS             complement(11425..12192)
                     /locus_tag="PC545_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457673.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNAIYVIDF
                     LDKNNLDNLKGNFYLIYQSHIDFEYLKNIFYNLFEDINIIQHNGFFIVNSKYNLDINV
                     TTQNIIETETYQSTYIFYLGELDSKADFDFRLQLCSDLLPHIIKDNAENKFLNLFDLI
                     RYKTLDLINEDNILNKLIDFNKIKSIDEELLYTGIKFINNDLNISKTSTSMFLHRNTL
                     VYRLEKINEILGFDLKNFENAMIFYLSVKSYFLYKKI"
     gene            complement(12222..13331)
                     /gene="ugpC"
                     /locus_tag="PC545_002918"
     CDS             complement(12222..13331)
                     /gene="ugpC"
                     /locus_tag="PC545_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDF2457674.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYENMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVILIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRVHIFDKETTLAIR"
BASE COUNT         5151 a         1518 c         2352 g         4375 t
ORIGIN      
        1 aaaatactta tttagataaa ataaataatt tttagagaat attttagaag ttacgattgt
       61 atgattttta aatgcatagg catttactct aggactatag actaatataa aaaaaaactt
      121 tgggaatact aaaaccataa atttataaaa ttaatatttt atatactgta aaacagtgag
      181 gagggacagt atgcatactc aactacaaaa gactattggt ctttcagcag ctctttctac
      241 tgttgtaggt atggttatag gttcgggagt tttttttaag ccacaagcaa tatacactac
      301 aactaatgga gctccaggtc taggtattat agcgtggctt ttaggtggtt ttataacaat
      361 aactgctggg cttacagcta cagaaatttc agctgctata ccaaaaactg gtggtatgat
      421 gatttatatc gaagaaatat atggagaaaa gttaggtttt ttaactggat ggatgcaaac
      481 tgtattattt ttcccaggta catcagcggc attaggagta atatttgctc aacaagcatc
      541 tgaactttta ggaatgagcc caaataatat ggcaaatgta cttcctatag caattggagt
      601 tatactattt ttagcgttat taaatataat aggttcatcc ttaggaggaa aagttcagac
      661 agtagccact attggaaaga tgataccttt aattttaata atagtctttg gatttattaa
      721 aggtcaaagt agtgaagtat taaatccatt tgtaggagat ggtgtaaatg catcaaatgc
      781 acttggacaa gtattaatag ctactttgtt tgcatatgat ggttggataa atgttggagc
      841 tatatcagga gaaatgaagt ctccagagaa agatttacca agagcaatag ttggtggttt
      901 atcattagtt atggcagttt atattataat aaatatagct tatctttggg tagtaccagc
      961 aagtgaatta gcaacagtta cttccccagc tacattagta gcaacaagat tgtttggaaa
     1021 tataggtgga aaagtaataa ctgtgggtat attaatatca gtatttggga cattgaatgg
     1081 atatttatta acaggttcaa ggatacctta tacattagct gaaatgggaa ctttaccagc
     1141 atctaaaact cttttaaaag taaattctgg aggttctcct gtaaattcaa tactacttat
     1201 aactgtgtta gcctgtgtat atgcattatc tggtcaattt aatcttttga cagacttaac
     1261 aatattttca atatgggtat tttatgtatt gacttttata ggtgttatga ggcttagaag
     1321 acaaaagcca gatttacaca gaccatataa agtcccttta tatccaataa taccaattat
     1381 agctatatta ggtggactgt ttgtaataat aaatcaaata ttgacttcaa cagtcatatc
     1441 tttaggcgga atatttataa cactattagg tttaccagtt tattattata tgaaaaaaag
     1501 ataactactt aatttaagtt agataaattt gggtaaaggt gaaaattaac ttttatccaa
     1561 attttttata ctgttataca atataagtat tatatattgc ttgatgtgct attttgtttg
     1621 atttataatt tagtaattga tgttttaaga taatttaaga aatgaggagt gaaagatgct
     1681 aggactttat gtacatattc cattttgtgt gaaaaaatgt aagtattgtg attttaattc
     1741 ttataaaatg gacatagatt caaaaaaaag atatatagaa gacttaaaaa tagaaatgga
     1801 attatatagt aataaattat ataaagataa taaatataaa aataaagaat gctgtagtct
     1861 aaataaaaat gacaaaataa cgagtatatt tgtaggggga ggtacaccta gtatactaac
     1921 ctctgatgaa ataagagaag tatttataag tataaaagaa atgtttgata tagatgagaa
     1981 tgcagaaata actatagagt gtaatcctgg gacattaaca ttagaaaaat taaaaactat
     2041 gaaagagatt ggcataaata gacttagtat agggctacaa gctattcaag aaaagcattt
     2101 gaactttata ggaagaatac atacttatga agaatttgag aaaaactata aagatgcatt
     2161 aagtgtaggt tttaaaaata ttaatataga tttaatgtac agtcttccaa atcaaactct
     2221 atgtgattgg aaggaaactc ttgaaaaagt tgtacactta aatccaacac atatatcagc
     2281 ttattccctt atactagaag aaggaactga actatacaat atgtatgaaa gtaataaatt
     2341 tgaacttata gatgaaaatg tagatataga aatgtatgag tatacaataa actacttaaa
     2401 gtcaaaagga tataatcaat atgaaatatc caactactca aaagaaggct ataattgtga
     2461 gcataatatt ttatattggg aatgtgaaca ttatataggg attggagcag gagcttctgg
     2521 ctatattaat gagaacagat acaataatgt agaatcttta gaagattatc atttaagttt
     2581 ggtaaaaaga gaaaaaccaa tacaagaaaa tgaaattctt tctgagaaag atatgataga
     2641 agaaaagata tttatggggc ttagaatgaa taaaggcata aaatttgaag attttaaaaa
     2701 aaaatttgga atagatttta gagagaagta taataagcaa atagaaatgc tattagctag
     2761 aaagcttata aatcagagct ttgaaggtat acaattaact caaaaaggaa gagaaatatc
     2821 taatagtgta tttatagaat ttatggaata atttttaagg tgttgacaaa ataaatttat
     2881 aatggtatat aatatatata atctttttag cactcgaact tagtgagtgc taacagctag
     2941 gaggtataat aatatggaat taaatgaaag aaaattaaat atcctcaaag ctatagttaa
     3001 agattatata gaaacagctg aagctatagg ttctagaaca atatctaaaa gacatgattt
     3061 aggtgttagt gctgctacca taagaaatga aatggctgac cttgaagaat tagggtattt
     3121 aattcagcct catacttctg cgggtagagt tccatctgaa aaaggctata agttatatgt
     3181 aaactctctt atgagtaaaa gcgaattgga tgataatgat aagattctta ttgaacaatg
     3241 tatgaatcac aatataaatc atataaaaga attgattcat gaaacttcta agctattatc
     3301 gcaactaaca aactatacaa ctgtagcagt tactaagagt ttaataaatc aaagtgttat
     3361 aaagcacata cagttggttg ctatgaatga caataatatt ttacttatag ttgtaacaga
     3421 taaaggggac ttaaaaaaag ctaaccttac aactaatgta tatttagatc aatctaagtt
     3481 aaatttgatt tctgataatc ttacaaggaa gctcttaggt aaaagtataa cagatttgga
     3541 tgataatcta attgctttta ttaaatacga aataagtgag tattctgggc ttatagatga
     3601 gcttttaaat gctttgaact caaatatgaa agaagaagat ttttctctat cattaaatgg
     3661 agctactaac atatttagtt atccagaatt taatgatgta ttaaaagcaa agtcattttt
     3721 aaatatgttg gaaaaaaagg aaacaatagc tgacataatt aaatcaaagg gaatacaaaa
     3781 agacaatcta aatataataa ttggtagtga taatgattgt gaactggcac aggattgtag
     3841 tatagttaca gcaacttata atgtagatag agacttggtt gggcgaataa gctttatagg
     3901 ccctaccaga atggattatg ctagaattta ctctataata aattatatga gtttattaat
     3961 taatagaaag taaagaaatt ttaaattgcg aggtgtaaag tcttggataa aaaaaatgag
     4021 caacaggaag taagagaaga aaatgatact tctattaacc aagaatcgga aactcaagta
     4081 gagttagaag aggaagttgt taatgaagaa tgtgaaacat caagtgaaaa aactgatgaa
     4141 aaagaagttg atgatgaaaa tgtaacagat ataaattcta agttagcaga aaaaaaattg
     4201 caggatgagt tggatgaatt aaatgataaa tatcaaagac ttcaagccga atatgcaaac
     4261 tacagaagaa gaactcaaca agagaaagag acaataggcg tatttgcaaa tgaaaaaata
     4321 ataactgaat tgataccagt tatagatagt atggaaagag ccttagatgc atgtgaagat
     4381 aaggaagata ctatgtataa gggtataagc cttgtacata aacaattaat agacacttta
     4441 gtaaaatttg gagtagaaga aatagaagca gaatctaagg aatttgaccc taatctacat
     4501 ctagcagtta tgcaagaaag tgtagatgga gttgaagcaa atcaaatagt aatggtactt
     4561 caaaaaggat ataagctagg aactaaagtt gtaagacctt caatggttaa agtttcttgc
     4621 taagtataaa ttaaaggtta aattatttaa cctaatataa aatattaaaa taagtaaaac
     4681 ttaattatat aattactcga ggaggatttt agtcatggga aaaataatag gaatagattt
     4741 aggaacaaca aattcatgtg ttgcagtgtt agaaggtgga gaagcacaaa taatagcaaa
     4801 tagtgaaggt atgagaacta ctccatcagt agtagcattt acaaaagatg gtgaaagaat
     4861 agttggagaa cctgcaaaaa gacaagcagt tacaaatgca gataaaacaa taacttctat
     4921 aaaaactcat atgggaactg attataaagt aaatatagat ggaaaatcat atactccaca
     4981 agaaatatca gcaataatat tacaaaaatt aaaatcagat gcagaaagtt acttaggaca
     5041 aacagtaaca gaagcagtta taacagtacc agcttacttt acagatgctc aaagacaagc
     5101 aactaaagat gctggtagaa tagcagggct tgatgttaag agaataataa atgagccaac
     5161 agcagcagct cttgcttatg gtatggataa attagaccaa gaaaagaaaa tattagtatt
     5221 tgacttaggt ggaggaactt ttgacgtatc tatacttgaa ataggagatg gaacttttga
     5281 agttttagct acagctggta acaatagatt aggtggagat gactttgacc aaatagtaat
     5341 agattattta gcagaggaat ttaaaaaagc tgaaggtgta gatttaagaa atgataaaat
     5401 ggctcttcaa agattaaaag aagcagcaga aaaggctaag aaagaattat catcaacaat
     5461 gagttcaaac ataaacttac ctttcataac tgctacagca gaaggaccaa aacacttaaa
     5521 tatagattta tcaagagcta aatttgaaga attaactaga ggtttagttg aaaaaactat
     5581 ggaaccaact aagacagctt tacaagatgc aggactttct acaggtgata tagatgatgt
     5641 attattagta ggtggttcta caagaatacc agcagtacaa gaagctgtta agaagtttat
     5701 aggaaaagaa cctcataaag gtataaaccc agatgagtgt gttgcagcag gtgcttctat
     5761 acaagctgga gttttagcag gggatgttaa agacttatta ttactagatg ttactccatt
     5821 atctcttggt atagaaacta tgggtaatgt aatgactaag ataatagaaa gaaatactac
     5881 aataccaact aagaagtctc aaatattctc aactgctgct gataaccaaa cagctgttga
     5941 tatacatgtt cttcaaggtg aaagaagtat ggcttatgac aatacaactt taggaagatt
     6001 ccaattaaca gatataccac cagcacaaag aggaatacct caaatagaag ttacttttga
     6061 tatagatgca aatggtatag ttaatgtttc agctaaagat ttaggtacag gaaaagagca
     6121 aaaaataact ataacatcaa acactaattt atcagaatca gaaatagagc aaaagataaa
     6181 agaagcagaa atgaatgcag aagctgacaa acaaaagaaa gaaaaaatag aagcattcaa
     6241 tcaagctgaa tctactatat atcaaactga aaaaacttta aatgaacttg gagataaaat
     6301 aagttcaggt gaaaaagagg atatagaaaa agctatagca gacttaaaag ctgtaaaaga
     6361 taatcaagat gctacagcag aagaacttaa gaaagctaca gatgaagtaa tgactaagtt
     6421 ccaaaaagta tctcaagaaa tgtaccaaaa agcagctcaa gaacaacaag cggcacaagg
     6481 tgcagaacaa gcacaagaca atggacctaa agatgacaat gtagtagatg ctgactttaa
     6541 agaagtagat gaagataaat aagctatata atatgtaaca ctaaatgata attggaacat
     6601 atatgatttg tgaaaatcat atagatgtta aagatatatg gacaatatac atcataaaga
     6661 atataatata tcatagatta tataacttaa agtagaatat aagaattaat gatagcttgt
     6721 agtttattat tctacaagct atcgttttgt aaaatgaagg tggtgtcaat ttttgagtac
     6781 aaaaagggat tattatgaag tcttaggtat cagtaaaggt gcagaagctc aagagataaa
     6841 aaaagcttat agaaaattag caatgaagta ccatccagat agaaatcctg gagataaaga
     6901 ggcggaagaa aaatttaaag agataaatga agcttatgaa gttctatctg atgatacaaa
     6961 gagaaaaact tatgaccagt ttggacatga tgggctaaat ggtcaaggcg gttttggtgg
     7021 tcaaggagga tttggcggtc aaggctttgg tggttttgaa gatatgttcg gagatatatt
     7081 tggtgacatg tttggaggaa gcttcggagg tggaagagca agaagaagag ggcctcaacg
     7141 tggtgcagat ataagacaga gtgttactat atcttttgaa gaagctacat ttggtaaaaa
     7201 gatgtctata aaagtaaata gaagtgaaga atgtgaagaa tgtaatggaa ctggagcaaa
     7261 accaggaaca tcaaagaaaa cttgttcaac ttgtaatgga actggtcaag ttagaactgt
     7321 tcaaagaact ccatttggta atatagcaag ttcaagacca tgtagtgctt gtaatggaac
     7381 tggagaagtg attgaatctc catgtagtaa atgtcatgga actggaaata caagaaaagt
     7441 taagactata gaagtagata tacctgctgg tatagatgat ggtcaaatga taaaactttc
     7501 tggtcaaggt gaagtaggag aaaaaggtgc tccaagagga gacttatata ttgtagttaa
     7561 tgttaagtca catccactat ttactagaga tggaaatgat atttattttg aaatgccaat
     7621 tacatttgtt caagcaactt taggtgatga gatagaagta cctactttgg atggaaaagt
     7681 taaatatagt gtgccagagg ggacacaaac tggaactgta tttagactta aagagaaagg
     7741 aatccctaga attagaggta attcaagagg agaccaatat gttaaggttg tagttgaaat
     7801 tcctaaaaaa ttaaacgata agcaaaaaga actattgaga gaatttgcga aggagtgtgg
     7861 gtcgaacgtc cacgaaaaga agaaaacctt cggacaaaag atagaagata tgtttaagaa
     7921 aaagtagtta aagtagccaa tatataatca tatttttaaa atcttagtta tttataagat
     7981 tgtcaaagga gatattatga tttttatatt ggctattttt gtataaaaaa taaatgtgca
     8041 taaaataaaa ctgaatataa ataattttct gaaaaatact tgtcctaaaa tatataatat
     8101 gctataatat aatcaaataa aagaaagggg tgattccagt gggaacggat gtggcactgc
     8161 caaatgatga agaaaacttt attgtaatat acaaaataaa attagatact tttagtgcta
     8221 gattaagtac tggaaaaact catgagctgg gataattatt gtaattacct agactcaaac
     8281 ttttagtagt aaaatagtaa tattatatag acaaggtatc taatttttaa atcttttgta
     8341 tgaattaaaa tttttataaa gggtatatct gattagaatg taattaggga aacctagttg
     8401 tgaattactt ttagtatata catgtgtaaa gaaatatgta tatggtattg ggtattgtgc
     8461 tagatgtgta tgtaaaggaa tatgtgtcta gttgggtata tgtaaagaaa tatacttata
     8521 atctatgaaa aatttttagt ataaattgct aaaacattga gattgttttt tatagaaagt
     8581 tagtttatac caactaaaaa tgtgcttaag atttgtttga tgtttaatta taaataaggt
     8641 tgtagataaa aaaataagta aagacaaagg tttaaaatac ttgctttaaa tatagtttgt
     8701 tttgaacact tgccaaatta aaataattaa taaaatagac atcttctcat agtaaaatat
     8761 actgtgagag gatgtttttg ctttgttcat aaacttttat gaaaaatttg catagtgtca
     8821 cattttaata tactatatat ataataaaat atagataaaa ttaaatgatt caaaagagaa
     8881 aggtgaaaag agatgtatta tataggtgta gacataggag gaacaggtat acaagcagga
     8941 gtagttgata actatgggaa gataattttt aggtcagagt gtaaaacagt aattgaaaaa
     9001 ggatttgaag gaatacttaa tgacataaaa ataatgattt ataagttgtt ggaagataat
     9061 aaacttacta tgagtgacat aaaatctatt ggatttggtg ttccaggttt tataaataaa
     9121 gaaggattag taacttgtgt aaatttaaaa tggaataaaa aagcttttaa taaagagtta
     9181 aaaaaaagat ttccagatgt tgaaattcat ggagaaaatg atgcaacagt agcagcactt
     9241 ggagaagcaa agtttggaag tatgaaaggt gctaatgttg gagtattata tactttagga
     9301 actggtgtag gtggaggaat agtaattaat caaaaagtct tttctggagc acatggattg
     9361 ggttcagaaa taggtcatca aattatagga gaaaactatt ttaattgtaa ttgtggaaat
     9421 aatggttgtg tagaaacttt ttgttcagca actgcaatta taaagtattc tcaaaaactg
     9481 atagaagaag gagaaaagag taggatttta gatttggcag agggaaattt agaaaatgta
     9541 aatgcaaaga tggtgtttga tgcatataga gaaaatgatt tagtggcaat taaagtaata
     9601 aatagattta aagagtattt agcaaaaact tttgcaaaca caataaattc tttagaccca
     9661 gaaattatat ctataggagg aggcatttct aaatctagcg atataatact tgatggaata
     9721 gaaggtttgg taagaaaatt tgtattatac aaaacagaag atatagctac aattacatgt
     9781 gcaactttag gttcagacgc aggaattata ggagcagctt ttttataatg taaatgaact
     9841 gcttattgga ggcgtgatga tatatgggta aaacaaatgc tagaagaacg ctttttttga
     9901 tagcaatagg ttcaggaact atgctgaatc ctcttaattc ttctatgatt tcacttgctt
     9961 tacatagtat acaaaatgat tttcatatta gttttacaac tgtatcttgg attgtttcag
    10021 cattttacct agctagtgct attgcacaac cagtttcagg taagatagga gatttaatag
    10081 gtagaaaggt cttatttttg agtggtttga tgcttgtaat tttatcagca tccatagtac
    10141 cacttgttca atcattcttt atattgattt ttataagagt aattcaagca attggaacaa
    10201 gtaccctata tccatctgga gttgctctta tacaaaataa tataaaagaa agacaatctt
    10261 ctgcattagc agttctaacc atttttgctt caactacagc aggattaggt ccgacacttg
    10321 gaggattatt gttggactta ggaggatggc atgctatatt tcttgtaaat atacctgttg
    10381 ttttggtttc tatatgctta gggtattttt tattcccaaa agaagtaaaa gaaaagaaat
    10441 ctataaaaga aactttaaaa aatgttttgt caagattaga tattattggc atacttctat
    10501 tttcaattgc aatgattttt atattactat ttttgctatc tatgaaggaa tcttttaact
    10561 tagaacaact tgtatttggt ataatactga tgtgtctttt tatttggcat gaattgagaa
    10621 caaaattgcc atttatagat attaagcttt ttgtaagtaa tcctaaattg tcaaaagttt
    10681 acttgcaatt tattatatta aatttcttta attatatttt gttttttggt cttcctagtt
    10741 attttcaaga tgccttacat tatagtgcaa agtcgactgg attatttatg ctatttatgt
    10801 ctggtatagg tatatttata tctccattaa ctggaaaatg gatagataaa tctggaacta
    10861 gatttcctct tgttacatca tcaatcttta tgtttattag tgcagtactt ctttcagtag
    10921 tttttatcca tatacctgta ataggaaagg gtattatatt atcattagca ggtattagtt
    10981 atggagttgg taatgtagcc ctgcaatcct ctatgtttga agaaagtcct cgtgacagta
    11041 tagggacagc ttctggtctg tttcaaacta gtcgttattt aggttctatt ttagcaactg
    11101 ttttacttgg aatggtattt gaagtaaata taacatcaga acaatttcag atattaggtt
    11161 atgttatggt ggtcttaggt acaataagtt ttttattaaa ttttatatta aagaaagaat
    11221 taagaaaagt agaatagtgg atttatggat ttaataagtc taataactga taagtttaaa
    11281 aatattgttt gtaaaacata actggtttga tatttattaa ttctaagttt tgaagcatag
    11341 agtactcgat aagtatacaa tattttgaat taaaattaag cagaaatttg aactagtctt
    11401 tcaaatctct gcttaattta gtagttaaat ttttttatag agaaaataag atttgacact
    11461 tagataaaaa atcattgcat tttcaaaatt ctttaaatca aacccaagta tctcattaat
    11521 cttttccaat ctatatacta gcgtgtttct atgtaaaaac atactagtag atgtcttaga
    11581 aatatttaaa tcattattta taaattttat tcctgtatat agaagttctt cgtcaataga
    11641 tttaatctta ttaaaatcta ttaatttatt gagtatatta tcctcattaa ttaaatctaa
    11701 agttttatat ctaataaggt caaacaaatt taaaaactta ttttcagcgt tgtcttttat
    11761 aatatgaggt agtaaatcag aacataactg taatctaaaa tcaaaatcag ctttactatc
    11821 taattcacca agataaaaaa tataagtact ttgataagtt tcagtttcaa ttatattttg
    11881 tgtagttaca ttaatatcta agttatattt agaatttact ataaaaaaac cattgtgttg
    11941 tattatattt atatcttcaa ataaattata aaaaatattt tttagatatt caaagtctat
    12001 atgagattgg taaattaaat aaaagttgcc ttttaaatta tcgagattat ttttatctag
    12061 aaaatcaata acataaatag cattattgaa agtaactaaa tggctactag catctaactt
    12121 ttggttttca tatggataga tatttatttt tttattaagc tgtctttcta aaaaatcttt
    12181 taactcttcc ataaagtctc cttaaaaata agattatatt cttatcttat agctaaagta
    12241 gtttctttgt caaatatgtg tactctacta ttgtcaactg aaagtgttaa gtcatcacca
    12301 acattaattc tgtttttact gtcaaatcta attgttatcg tattttcgcc tagttttaag
    12361 tgagcataag tttctgctcc cataagttct tttatttcaa ttcttgattt aaaacaagta
    12421 tctggattag atttaataaa agtatcttct acatgaatat cttctggtct gatacctata
    12481 acaacttcat tattaatata attgtctttt attaaatcat agtgtttttc tttattcaat
    12541 ttaagcttaa tatgttcatt ttgtgcataa atttcatcat tttcttctat taatattaca
    12601 tcaataaaat tcatctgtgg tgctccaata aatcctgcaa caaatatatt agctggataa
    12661 tcgtatacat cttgtggagt tgcaatctgc tgaactactc catccttcat tactacgatt
    12721 ctatcagcca tagtcatagc ttcaacttgg tcgtgtgtta catagataaa tgtagttcca
    12781 agctgttggt gtagcttagt tatctctgtt ctcatagcag ttctaagttt tgcatctaag
    12841 ttggatagag gttcatccat taagaatacc ttaggatttc taactattgc acgtcctaag
    12901 gcaacccttt gtctttgtcc tccagataat gttttaggtt ttttatttaa aagaggtagt
    12961 aagtctaata ttttagcagc ttctttaact tttttgtcta tttcatcttt aggcaatttt
    13021 ctaagtttaa gtgcaaatgc catgttttcg taaacagata agtggggata taaagcatag
    13081 ttttgaaaaa ccatagctat atctctatct tttggttcta tatcattgac aagtttatct
    13141 ccaatgtata gttctccttc agagatttct tcaagaccag caatcattct aagtgtagtc
    13201 gatttaccac aaccagatgg tcctactaaa acgataaatt ctttatcttt aatgtctata
    13261 tttatatttt ttactgcatt aaatccattt gagtaaagtt ttgatatatt ttttaaaatt
    13321 acttctgaca taatatcctc cttgatattt aaattttaat tatatagtat caaatttttt
    13381 tattatatta aatgtg
//
LOCUS       DAMJVI010000097        13324 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.102, whole genome shotgun sequence.
ACCESSION   DAMJVI010000097 DAMJVI010000000
VERSION     DAMJVI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.102"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            176..2035
                     /locus_tag="PC545_002919"
     CDS             176..2035
                     /locus_tag="PC545_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2457675.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHQPNDELIEYIDEILQELITGHGDYGLVITDCNEYNEDLLLLEKKW
                     EDLNIEIKKVRMKEQNNQLLSISEEFFSLANDTVFKIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMVRLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICIHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YVDKEDLVVRQRDVDNELINITNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHSQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQKHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDVLKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIECKEQVEFLRDIGCDLVQGFVFYKPMPILEFEKILDKEFVYNS"
     gene            complement(2327..2926)
                     /locus_tag="PC545_002920"
     CDS             complement(2327..2926)
                     /locus_tag="PC545_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2457676.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGNDRTGSNTQGENLRIN
                     GNKIKDIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(3029..3826)
                     /locus_tag="PC545_002921"
     CDS             complement(3029..3826)
                     /locus_tag="PC545_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2457677.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(3939..4044)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(4136..5122)
                     /locus_tag="PC545_002922"
     CDS             complement(4136..5122)
                     /locus_tag="PC545_002922"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDF2457678.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMDYLSKTNTDNTNYCLSEHD
                     IKQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVAFVENKLIGIRHEENALRNTLDSIEINDYFLGADTDILVSGILGVK"
     gene            5606..7336
                     /locus_tag="PC545_002923"
     CDS             5606..7336
                     /locus_tag="PC545_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDF2457679.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIVISALLLKEFGPMRKAEIKSRSRKISI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGDDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVVKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKK
                     VDEAEIELVE"
     gene            7446..7526
                     /locus_tag="PC545_002924"
     tRNA            7446..7526
                     /locus_tag="PC545_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7480..7482,aa:Leu,seq:tag)
     gene            7861..8376
                     /locus_tag="PC545_002925"
     CDS             7861..8376
                     /locus_tag="PC545_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HDF2457680.1"
                     /translation="MEKYIWLFPLLFIFHDMEEIIGFGIWLKKNKSMLDKKYPFISKV
                     YENYSTEGMAFAVFEEFILCIIFCILTVITENQYVYLLWLGSFIAYTLHLVIHIGQSI
                     IIRKYIPSLITSIICLPISIWCISKSIYIVDCEMSTTILYSIIGIIIVALNLKFAQSL
                     IGKFTKWMSNI"
     regulatory      8868..8972
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            9182..11656
                     /gene="gcvPA"
                     /locus_tag="PC545_002926"
     CDS             9182..11656
                     /gene="gcvPA"
                     /locus_tag="PC545_002926"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HDF2457681.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHEKVRK
                     SAGIFDVSHMGEVQIKGAESEKFIQNLVTNDISALKINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSEGYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSTKVCGCPCLVSRTGYTGEDGFEIYCKNKYVEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPIEGGLSIFVKTNKESFIGKSILSK
                     EKESGAKRKLVGFEMQGKGMPRNGYDIRIGDKTVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIVKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATAAIEACIMAMNQTRKSKIVVSKTIHPETLSILRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDVEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYIATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAVQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            11656..13113
                     /gene="gcvPB"
                     /locus_tag="PC545_002927"
     CDS             11656..13113
                     /gene="gcvPB"
                     /locus_tag="PC545_002927"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HDF2457682.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIENMIDQDMARQSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLAEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGDMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVPFLPIPVIERKEDKYVLNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIEAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         4995 a         1576 c         2199 g         4554 t
ORIGIN      
        1 tatattagaa aaaattttgt atatatagag gactctaaag atgaactatg taaaacaagg
       61 tttatatatt ctaattgtat agaaaattta ttgtaaacgc atgtatatta ttagaaaaaa
      121 tagtttgaag gtattcaata aacataaaaa acaatatagg ggataaaggt gttttatgaa
      181 gagatttttg aggagaatta tcttagtttt atttattttg ttgattttta taagttttat
      241 ttctattaaa ttaattcata atgttggaga ttatggaaaa ttgattaatt atgttgggat
      301 tgttcgtgga gctagccaac gtctaactaa actagagatg aatcatcaac caaatgatga
      361 attaatagaa tatattgatg aaatcctaca ggagttgatt acagggcatg gagattatgg
      421 tttagtaatc actgattgta atgaatacaa tgaggactta cttttattag aaaaaaagtg
      481 ggaagattta aatattgaaa ttaaaaaagt aagaatgaaa gaacagaata accagttatt
      541 aagtataagt gaagagtttt tttctcttgc aaatgataca gtgtttaaaa tagaaaactt
      601 ttctaaagaa aaaagtaatt acttaatgac tttaataata attatatcta ttataggtat
      661 attagcatgt atcatattga ttttacaata tagtaaaaaa atggttagac tggaaaaatt
      721 aaatgtagat ttaaaaaata ttgcatataa agatgaacta acaggagtaa atactataga
      781 aaaatttaaa ttggatgcaa atcaaaatat ttgtatacat aaggataaga aatttgctgt
      841 attctatatt gattttgaaa actttaaata cattaatgat atatttggat atgattatgg
      901 tgacatgata ctgaaaagat atgctaatct tatgatgaat gacataggga aatatgaaat
      961 atttgcaaga gaaattgcag atagatttgt tgcgttaaga tgctatgtag ataaggaaga
     1021 tttagtggta agacaacgag atgtagacaa tgaactaatt aatataacaa atgaaattaa
     1081 aaataaacat agtattactg ttgttagtgg aatttgttgt attgaagatg taaatgaaaa
     1141 attaagtata gatggtttaa taaatagagc aaattttgca caaaaaactg ttaagaataa
     1201 accaggtact aattatgctt tttataatga cagtattcgt aaaaaaatga ttgaagaaaa
     1261 tactatcaag agtagaatac atgaagctat tgaaaaaaga gaatttatag tttatttaca
     1321 gcctaaagtt aatttacata gtcaaaaaat aaattgtgca gaagctttag ttcgttggct
     1381 tactccggat aaaggtatta tatcgccagc aatatttatt ccagtacttg agaagaattt
     1441 ttttattgcc ttagtagata agtatgtttt tgaggaggtt tgtaaatgga ttagaaagag
     1501 gttagatgaa aataaacctt ttgttcagat atcagttaat gtttcaagaa tacaatttta
     1561 taacactaaa tttgtagaaa cttattccaa tatacaaaat aaatacagga tacctaaaaa
     1621 tactatagaa attgaattta cagagagtgt tgcttttgag aatcaaaaac atcttttgga
     1681 aattatacat gatttgcatg aaaatggatt tacttgttct ttagatgatt ttggaaaagg
     1741 ttattcgtct ttaagtgttt taaaagattt accatttgat gttttaaagc ttgattctat
     1801 gtttttcaag gctagtttag ataaagataa agagaaaata gtaataaaaa atattgtaca
     1861 tatgttaaaa gaacttaata tcacaactgt tgctgagggc attgaatgca aggaacaagt
     1921 tgaatttctt agggatattg ggtgtgattt agttcaggga tttgtatttt ataaaccaat
     1981 gccaatatta gaatttgaaa aaatattgga taaagagttt gtttataata gctaaaatgt
     2041 actttataaa atatgaacta attaagtaaa attattttgt actgaataaa tttttacttt
     2101 atttacagaa taatgattat ttacagaaaa gcgcgaaaag tgtattcctt taagtgtggg
     2161 atggatagca caaatagagg agaaaaaata ttggccatat aagaatgcat aataatataa
     2221 atatttgaaa ggtcagcatt atataaaaat taaaaagaaa ataaataatt tttctataaa
     2281 aaagagacta tgagaaaatt tactcatagt ctcctaaata atgtatttat tttcttcctg
     2341 caacccattt taatccccaa tgaaattcag tatccatttc tctatgacct ttgaagtatt
     2401 taactacttt ttcaacactg aatgtctcat catttacatt ttctatcaat gctattgaac
     2461 acatattata gccattttta tgttcatcaa gtttgacaac taagtctgaa tcttgttttt
     2521 gtttgattgt tacaattcca tcagcttcag accagttagc tacaccttcg tatataaaag
     2581 cataaataag tattcttttt atatctttta ttttattacc atttattctt aaattttctc
     2641 cttgtgtatt acttcctgtt ctgtcatttc catctaattg agcaaaaggt ggattgtcta
     2701 aactaccaaa ggcatttcct aatgcttgaa cagctccttt tacaccattt ttcatctcaa
     2761 ataggcaacc caaatctaaa tctatattac tgcttcttaa agagccaaaa aaaccctttt
     2821 tttgtacttt ttgattccaa tttaaattaa ctaagatttc acctaaatcg ctattttcct
     2881 tttttgtaag gtttattttt tgtccctttt ttaattctat agccatagta atcttcctcc
     2941 acaaacatag atttatttaa atggtagcac ttaataagtc aatattcaac aagattagaa
     3001 taataatatt aaaattatac taaatatact aaaaagtagg aatcacaata cctttgtatt
     3061 tttcttcaat taagcttctt gctttatctg atgtaagaac atcttgtaga actttaatct
     3121 tttcactgtc tttatcttca gttctaaatg ctaaaagatt tgcatactcg tcatttatag
     3181 gagctgtata aattccgttt ttcttggcat caagaccagc aggtacagca taagtagtat
     3241 ctataaatgc agcagtcaca tctggaagag aagatggaat ttgagcaata tctaactctt
     3301 tgaattgtat tccctttgga ttttctatta tgtccttagg actaactaat ccatcccctt
     3361 taggtaacgt taaaagtcca atactttcaa ggtatctaag gttcttagaa caagaagatg
     3421 ggttattgct tattgcaatt gtatctcctt ttttaaattc atcaacagat tttatcttat
     3481 aagagtaaag tattgctggg catacatata gttttttacc agccattatt ttgtaacctt
     3541 tggccttaac agcttctttt agataaggtt catgttgaaa tagatttgca tcaatttcac
     3601 cattatttaa agcttcgttt ggaagtatgt aatcatcaaa gtttttaact tctaaagtat
     3661 atcctttttc tttaataagt ggttttaatt cttctaataa ctctcctcct ggaactaaag
     3721 tagcaccaac aacaattttt ttatcatctt tagctttaga acatccaacc acagctaaag
     3781 ttaatactag acataataac aaacataata attttttgaa tttcatataa attccctcct
     3841 gaatataatt gattagttaa aaataaaata ccattcttta tcacactgca aaataaaaat
     3901 aagcctgagt ttaaactcag gctttaaaag ctaatttaga ctcatctttc agtataatac
     3961 tgcaggattt agcaccaatg tatataaaaa tatactggtt gctgggtttc atagggccag
     4021 tcccttcacc actctggata agaagtatca atttttaatt atgttataaa tattatcatg
     4081 attttttacg tctgtaaaca taattttata aatattttaa aatctttaat gtattttatt
     4141 taacacctaa aattccagaa actagaatat cagtatcagc acctaagaaa tagtcattta
     4201 tttcaataga atccagagta tttctcaaag cattttcttc atgccttata ccaataagtt
     4261 tattttctac aaatgcaaca tcacattttc caaaaaaatc tccaaaaaat tttatactag
     4321 ttattatgcc tttatcaaca tttaaattaa actcaacatt tccaccagaa tattttaatt
     4381 catttgaaag agtatatttt ggtgaattac caaaattcca atcccaagtt tcgtattttg
     4441 atttagtaag cttttctatt tgttttatat catgctcact caaacaataa ttagtattat
     4501 cagtatttgt tttagaaaga taatccatta tagcatcttt aaattctaat atgtccattt
     4561 ttgaatgtaa atgttcactt atattggtta cacgagattt tacagatttt attccttttc
     4621 cttcaaattt tataggttta acctttaatg cttttgagag gtcatttatt tcagatgaaa
     4681 ataaaagagt accatgatgc attactttgt ttttgtagtt gtattgagca tttccagaaa
     4741 atttcttgcc atctataaga aggtcatttc ttccagaaaa ttcggcattg acatctagtg
     4801 ttttaagtaa atctattatg ggctgagtga atcttctgaa atcactaaaa ttattattgt
     4861 tacaagctat aaatgtaaaa tttatatttc ccaaatcatg aaatacagct ccaccaccag
     4921 actgtctcct aacgacaggt atagaatatt cttttatata ttctaaattt atttcagaaa
     4981 gtgtgttttg gttttttcca acaattatag aaggttcatt cctccataaa ataaataagt
     5041 cttcatcaaa attttttaga agatattctt ccattgctaa attaaaataa gggtttgttt
     5101 tttcattata aattaataac attaaagtag ttcctccata gaattgataa aatttttatt
     5161 atgaatacaa ttaattttat aattcgacaa taatataaaa actcctttaa attagaaatt
     5221 ttaaaaacta ccttgcaaag tttaaataaa taatatatac tcaaaatatg tttaataaaa
     5281 gtgaatagaa gggtagaggc gcaataatct atcagtacta ataaggagtt aagcacagag
     5341 atttattagg aaaggagata ttgccgaagt tttataatcg atgctttaag attatattgc
     5401 tgggggtaca tagaaaatat gtacaactgt cacaggttta tttgtggtga gctatcattt
     5461 aaggaattat attatattta tttttactat tgttgatata ttgtaatgtt taagccttga
     5521 gtgtttacca ctcaaggctt ttttagtttt attgtaacat aataccctgt acagttcaca
     5581 aaattaaaag gagagggaga tttatatgag gaacaaaaaa attaatatta tttttctgac
     5641 gacaatcatg ttcataatgt caacagtaat ggtttttgca gaagaagata tagacactat
     5701 tgcactagcc aatgcagaaa aatttgggat tttaacttta ataccaccat tagttgctat
     5761 aatacttgca tttataacta aaaacgtaat aatttcttta cttataggta ttctttctgg
     5821 tagttttata attaaagcta gtggtattaa tgtttttgcg acatttatac aggcattttt
     5881 agatttagta gacagagctt tagtatcttt agcagaccct tggaatgcag ggataatact
     5941 acaggttttg gcaattggag gagtaataaa tcttgttgct aaaatgggag gagctaaagc
     6001 tatagctgaa gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc ttataacatg
     6061 gtttttgggg cttttagtat tctttgatga ttatgccaat tcactaatag taggtcctat
     6121 gatgagacct gttgcagata aaatgaaaat atcgagagaa aagctagcat ttataataga
     6181 tgccacagca gcaccagttg ctggtttagc tataatatct acatggatag gtcttgaggt
     6241 tggtttaata catgatgctt ttgaaagtat aagtatagat gtagatgcat ttggtatatt
     6301 tttaaataca ataccattta gattttacaa tatattaata ttagctttca ttgtaatatc
     6361 agcattatta ttaaaagaat ttggtccaat gagaaaagct gagataaaat ctagaagtag
     6421 aaaaattagt atagaccttg atgaaggcgt agaagagttg gatgatttag ctccaaaaaa
     6481 tggagtaaaa ttgagtgtat ggaatgctat aatcccaata ggaacattga taatagttgc
     6541 attagcatca ttttattata gtggatatac atctataatg ggaggagacg ataaggctct
     6601 gatacaacta tttacaaatt ctccatattc ttttgaagca ataaaagaag catttagtgc
     6661 atctgatgca tctagagcat tattccagtc tgcacttgtt gcaagtttag ttgctattat
     6721 aatggctgta gtaaaaaaga tttttacaat atcagaagca attgatgttt ggatagatgg
     6781 aatgaaaagt ttagtaatca ctggtgtaat attaatactt gcatggtcat taagttcagt
     6841 aataaaagag ttaggtacag ctaaattttt aatacaccta ttatcaggtt cactaccacc
     6901 attcttatta ccaagtttaa tatttggttt aggagcaata atatcttttg ctactggaac
     6961 agcttatgga acaatgggta tacttatgcc acttgcaatt ccattagcat actcattaaa
     7021 tcctgatatg tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg caatatttgg
     7081 agaccattgt tctccaattt cagatacaac aatactttct tcaatgggag ctggctgtaa
     7141 tcatatagac catgttaaca ctcaaatgcc atatgctata tttacagcag taatcactat
     7201 tgtatttggg tatataccag ctggacttgg attgccaata tatatagtat taccagtggc
     7261 aatagctgct atatttgtag gtattcaaat aattggtaag aaagttgatg aagcggaaat
     7321 agaacttgta gaataaagat ataaataagt ttcacctttt attatcacta ttttgttagt
     7381 ttttagataa ttttaaaaag ttattgaaaa taatggcgaa atatagtaaa ataaaagagt
     7441 gataagcgga tgtggcggaa ttggcagacg cactagactt aggatctagc gccattggcg
     7501 tgggggttcg actcccttca tccgcacttt acagtactta attgtactga aaaaaactgg
     7561 ggttgctggc tttcggagtg ggctatcccg tttttatttt tttgtatata taatagattg
     7621 gcactttagt ataaggtgct ttttttatag gcaaaaacag tacatttatt tctgttttat
     7681 tatcattcat ctattagaat atactttagt taagaatgtg ttaataagca gtgttcacat
     7741 tcttgataaa atgtggaata atatgtattt ttactaagac ggaaatgaaa taattaaacc
     7801 attaaataat attataagat gatgaataag tagataaatc aaattttgta ggaggatatt
     7861 atggaaaaat atatatggtt gtttccatta ctttttattt ttcatgatat ggaagagata
     7921 ataggttttg gaatatggct taaaaaaaat aaatcaatgc ttgataagaa gtatccattt
     7981 atcagtaaag tttatgaaaa ttatagtact gaaggcatgg catttgctgt gtttgaagaa
     8041 tttattttat gcattatttt ctgtatactt acagttataa cagagaatca gtatgtatat
     8101 ctactatggt taggtagttt tatagcatat accttacatt tggtaattca tatagggcag
     8161 tcaatcataa taagaaaata tataccttca cttattacca gtatcatatg cttaccaata
     8221 agcatttggt gtataagtaa gagtatttat atagttgatt gtgaaatgag tacaacaatt
     8281 ctgtacagta taattggcat tataattgta gcattgaatt taaagtttgc acagtcgctg
     8341 attggaaaat ttacaaagtg gatgtcaaac atataaattt tagtttattg gacagttcaa
     8401 ggaatcagaa attggaattt tatagtatta atattttatg gtattaatat aatgtataaa
     8461 attttatagc aatgtatttc ataagtataa agtgactaga attttaagaa atatacaaat
     8521 tctataaaaa ctaaataatt tcaagtatat tttaaaaatc ttatttttca caagtttaaa
     8581 attgtaaacg atttcatcaa gtctatgaat atacttttaa catgttatag taaatctcaa
     8641 tataaattta catttggttc tttgtgtgtt attttaataa taaattaagt gaaaatgtta
     8701 ttttacatta atatataata tctttgcata agagtgaata atataaaaga gaaataattg
     8761 ataataaaaa cttaacaaaa aaattaatac ataaaaaaaa ataaatataa tgtgttgaca
     8821 tttaattgaa atattcatac aataaaattg aaaataaata tttccaaatg agattagcga
     8881 gagagtcctt tatacgaaaa ggcgccgaag aagaaattta agaaatgcca atttttagag
     8941 aagctttcag gcaaaaatat cgctaattga tgggactctg gagagactta agttaagata
     9001 ctataagtca ccgaagaagc aaaataatat taaattatta aatctttcag gtatacaaac
     9061 agggatatat aggctaggat tattttccat gctgatatat ctttttttta tgaaaaagta
     9121 caggaaataa tatcaaagtt acttaaatac agtaggtcta attttctagg gagggttata
     9181 tatgaatgaa ttgaaaagag tatctttgta caatatacac aaagagcttg gagcaaaact
     9241 tgttgaattt gctggttggg aaatgccttt agaatatgag ggtataaata aagagcatga
     9301 gaaggtcaga aaaagtgcag gtatatttga tgtatctcat atgggagagg tacagataaa
     9361 gggagcagag tcagaaaaat ttatacaaaa cctagttaca aatgatataa gcgcattaaa
     9421 gataaatgat attatatata cacctatgtg ttatgaaaat ggtggagtag ttgatgattt
     9481 attaatatat aaatttggtg aagaagatta tttattagta ataaatgcag gtaatataga
     9541 taaagatgta gcatggataa taaaacaaag tgaaggatac aatgtagata taaaaaatat
     9601 atctagtgaa gtgagtcaat tagcaattca aggacctaaa gcagaagaaa ttcttcaaaa
     9661 aatcacagat atagacttaa actcaataaa attttataag tcaattccat caacaaaagt
     9721 atgtggttgt ccttgtttag tttcaagaac aggatatact ggggaagatg ggtttgagat
     9781 atactgtaaa aataaatatg tagaaataat ttggaatgaa gttctaaagg ttggaggaga
     9841 agatatatgt ccagctggtc taggttgtag ggatactttg agatttgaag cagctctacc
     9901 tctatatgga catgaaataa atgaacacat atctccaatt gaaggtggtt tatctatctt
     9961 tgtgaaaaca aataaagaat catttattgg caaatcaatt ttatcaaaag aaaaagaaag
    10021 tggagctaaa agaaagttag taggatttga aatgcaaggt aaaggaatgc ctagaaatgg
    10081 atatgatata aggattggtg ataaaacagt tggatttgta acaacaggat gtgcatctcc
    10141 aacgacagga aaaattttag gtatgggaat tatagattct gaatatgcaa aagtaggaaa
    10201 tgaaataggt atagcaatca gaaaaaaagt tgttcctgct gtgatagtta agaaaccttt
    10261 ttataaaaaa caatataaaa aagataatat tatattaaac aaagaaaata aattttcata
    10321 tatacctgct acaagtgaag ataaatcaaa aatgttaaaa gtagttggat taaattcggt
    10381 tgatgaatta ttttcagata taccagaaga agtaaaatta aaaagagatt taaatttgga
    10441 aattggaaaa tctgaattag aggtaagcaa aatagtaaaa agattgtcag aagaaaattt
    10501 aagtttagaa gacttgactt gttttcttgg tgctggtgca tatgaccatt atataccatc
    10561 aattataaaa catataactt caagatctga attttataca gcatatactc catatcaagc
    10621 tgaaataagt cagggaactt tacaagtagt ttttgaattt caatctatga ttgcagaaat
    10681 tacaggtatg gaaattgcaa atgcatcaat gtatgatgga gcaactgctg caatagaagc
    10741 atgtataatg gcaatgaatc agactagaaa aagtaaaata gtagtgtcaa aaactattca
    10801 tcctgaaact ttgtcaatat taagaactta cttacaatat aaagattgtg agatagtaga
    10861 aattgacttt tgtaatgaat atggcactac agatatagaa aaattaaaag cttctgtaga
    10921 caaagataca gcatgtgttc ttatacaaac tcctaatttc tttggaatta ttgaagaaat
    10981 ggaagaaata gaaaagataa ctcatgaaaa taaggctatg ttaattatga gtgtagaccc
    11041 aatatcatta ggagttttaa aaacaccagg tgagataggt gcagatattg ttgttggaga
    11101 agctcaatca cttggaaacc ctcttaattt tggaggtcct tatgtaggat ttttagctag
    11161 caaatctaaa tatactagaa aaatgccagg aagaatagtt gggcaaagtt tagatgtaga
    11221 aggtaaaatt gcatatgtat taactttaca aactagagaa cagcatgtaa gaagagaaaa
    11281 agcaacttct aatatatgtt caaatcaagc tttaaatgct cttgtagctt caatatatat
    11341 agctactatg ggtaaagaag gatttaaaga ggttggtatg cagtctatga aaaaagcaca
    11401 ttatacttac aataagcttg tacaaactgg aaaatataag ccaatattta aaggtaagtt
    11461 tttcaaagaa tttgctgtac aaggaaatct taatatagaa actataaatg ataaactctt
    11521 agaagaaaat atattaggtg gttataattt agaatacaat tatccagaat taaaaaattc
    11581 aactttgctt tgtgtgactg agaaaagaag caaagaggaa atagataagt tagttggaat
    11641 aatggaggga ttataatgaa agaatacaat agtttgttga tagatatatc taaaaaaggt
    11701 aggaaagcat actcacttcc aaagttagat atagatgata taaaaataga aaatatgatt
    11761 gaccaagata tggcaagaca aagtgagttg aatttgccag aagttggtga attagaatta
    11821 gttagacatt atacattatt atcaaataaa aactttggag ttgatacagg tttttatcct
    11881 ttgggttctt gtactatgaa atataatcct aaaataaatg aagatatggc agcacttcca
    11941 aactttactg gaatgcatcc ataccaatct tcggatacag ctcaaggttc tctttctttg
    12001 atgtatgatt tatcaagaag acttgcagaa attacaggta tggatgaagt tacactacaa
    12061 ccttcagcag gttctcatgg tgagtttaca gggcttatga ttataaaagc atatcatgaa
    12121 aatagaggtg accataagag aaccaaagtg ataattccag attctgcaca tggaacaaat
    12181 ccagcaagtg cagctatggc aaactttgat gtgattcaaa tagcatctga taaaaatgga
    12241 gctgtagaca taaatgcatt aaaagaggtt ttgaatgatg aagtagcagc tcttatgctt
    12301 actaatccaa gtactcttgg tttatttgaa aaaaatataa aagaaatagc tactcttgta
    12361 catgaagcag gaggactttt gtactatgat ggtgcaaaca tgaacgctat tatggggata
    12421 acaagacctg gagatatggg gtttgatgtg gttcatctta atcttcataa gactttttca
    12481 actcctcatg gaggtggagg tccaggagct ggtcctgttg gagttaaaaa agaattagta
    12541 ccttttttac caataccagt aattgaaaga aaagaagata agtatgtact aaattatgat
    12601 agagaaaaat ctattgggaa aattaaaaat ttctatggta attttggagt tcttgtaaga
    12661 gcttacacat atatactaac tatgggaagg gatggactta aagaggctag tgaaatggca
    12721 gttttaaatg ctaattatat aaaggaaagt ataaaagatg attatatttt gccaatagat
    12781 accttgtgta agcatgagtt tgtattaggt ggattgccaa gaggagaggc caatataaaa
    12841 actatcgata ttgcaaaaag actactagat tatggatacc atcctccaac aatgtacttt
    12901 cctcttatta taaatgaggc acttatgata gaacctacag aaagtgagag tatagagaca
    12961 ttagatagtt ttatagaagc tatgaaaagt gttgctaaag aggcaaagga agaaccagaa
    13021 ctattgaaaa ctgctccaca taatactttg gtcaaaagag tagatgatgc aagggcagta
    13081 aagaaaccta tactaacatg gtcacaaaga taaatagtta aaaagtagag atttaaagta
    13141 taaagagaat taaatttgta agaaaaagct atctaactta atctagttta gatagctttt
    13201 ttgtatatta gtatgtatgg tattttctat ataaagtttt aaattatatt agtgcttata
    13261 tagaatattg atatgatgat tatgtataga tattaataag aattgttgaa tttatcttgc
    13321 ctta
//
LOCUS       DAMJVI010000098        13126 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.103, whole genome shotgun sequence.
ACCESSION   DAMJVI010000098 DAMJVI010000000
VERSION     DAMJVI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.103"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            202..1083
                     /locus_tag="PC545_002928"
     CDS             202..1083
                     /locus_tag="PC545_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457683.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPAKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYL
                     PPAIKNFKNFVIKNAQL"
     gene            complement(1291..2154)
                     /locus_tag="PC545_002929"
     CDS             complement(1291..2154)
                     /locus_tag="PC545_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HDF2457684.1"
                     /translation="MLSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPIV
                     EPVGPWLEKMR"
     gene            complement(2309..4243)
                     /locus_tag="PC545_002930"
     CDS             complement(2309..4243)
                     /locus_tag="PC545_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HDF2457685.1"
                     /translation="MKKSLKKYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKVIDGKTYYFNSDGVMQTGWQEKNGKKYYLGSDGLAV
                     TGWQEIDGNKYYFDKTGIMQTGWQQIDGKSYYLDKDGKMLTGSQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTANLPTETVEEMKLLAPKTVILIGGENAISSKLEQEIKTTFNTETKRIAGQDR
                     YQTATRIAEELGNREEIKTAYMVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAINDSVIAKMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDTDLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMDNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILAK"
     gene            complement(4355..5368)
                     /gene="galE"
                     /locus_tag="PC545_002931"
     CDS             complement(4355..5368)
                     /gene="galE"
                     /locus_tag="PC545_002931"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDF2457686.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNSIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHVVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLAAGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5392..6261)
                     /gene="galU"
                     /locus_tag="PC545_002932"
     CDS             complement(5392..6261)
                     /gene="galU"
                     /locus_tag="PC545_002932"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDF2457687.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIYCAKSFIGDEPFAVLLGDDIVDSDVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFSILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6459..6779)
                     /locus_tag="PC545_002933"
     CDS             complement(6459..6779)
                     /locus_tag="PC545_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457688.1"
                     /translation="MSSKYNVWTFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSGLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYLDEDIDSDIKGI
                     LDEI"
     gene            complement(6769..8760)
                     /locus_tag="PC545_002934"
     CDS             complement(6769..8760)
                     /locus_tag="PC545_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457689.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNEDTGVKYIYSFSDNKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKFYMGGINKDNEKFIFISGKNRFEIN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCEIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLKDIENEIVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKVLLNLSKSMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEQRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8883..11426)
                     /locus_tag="PC545_002935"
     CDS             complement(8883..11426)
                     /locus_tag="PC545_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HDF2457690.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSERRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVPNNVEENNEQNKVEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKSNDIESKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEAYGFGKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAQNLIKDNYSELENHEIASKRTRRNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDAEEGTKEEVDSNNQDIGDVVEDKDTTDKEYDSNKEDIIE
                     PENKKSKKKAKLFGFIKKDNEEAEQEEENLNDISPDIILDKPVENHQVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSSNNESKLEKESKNLEDKKAKEIKEE
                     KLENEKSVVIPIKKKENSNKKSKNSSKDKYRENKKEMKNYVSDKEDSLDDEEVVSKKS
                     RLKETIIAVVIVVIVGVGYFITVGNNKKNDKENIPKSSTQQQANNKLTEEEKKAQAEK
                     EKKEAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGVYYTVYAGSLKVEKTAKETAKE
                     YEAKGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLE
                     ELKESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11704..12038)
                     /gene="rnpB"
                     /locus_tag="PC545_002936"
     ncRNA           complement(11704..12038)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PC545_002936"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12170..12847
                     /gene="srtB"
                     /locus_tag="PC545_002937"
     CDS             12170..12847
                     /gene="srtB"
                     /locus_tag="PC545_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HDF2457691.1"
                     /translation="MKKLYRIVINIILVLVILYSGFNIYSKLTKYNHDTKISSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSIDDKNIIIYGHNMKNKTMFNNLNKFKDADFFKKNNKIKITLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNNESDYQNYINDITSKSLYKSPIKVNSNDKIVTLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13007..13081)
                     /locus_tag="PC545_002938"
     tRNA            complement(13007..13081)
                     /locus_tag="PC545_002938"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13047..13049),aa:Thr,seq:tgt)
BASE COUNT         4045 a         2297 c         1338 g         5446 t
ORIGIN      
        1 tttatttata agataattct tgtatttttc tattaggtcg tataaatata tttatcttat
       61 aattcagaca aaaaacgata aattttttta aaagctttta ttcttactct ataatcatat
      121 ataataaaaa tgtaaaaata tcttaaaggt taacttctac aaatatataa ataaaaatac
      181 ctatgaaagg atgaacaaaa attgaattta tatcacttac gctattttgt tacattggca
      241 catttagaac actatacaaa agcggcagaa aatttatcta taacacaacc aagcttaagt
      301 catgctattt ctttactaga aaatgaactt ggggtggctc tatttgaaaa agaaggtaga
      361 aatatagtcc ttactaagta tggaaaaata tttttaaaag atgtggaaaa atcattagaa
      421 attttagatt caagtgtaaa atcattaaaa ataactggta ctggtgaagg acaaatagac
      481 ttagcttttc ttagaacact tggtactgat tttatcccag acatagtgca caaattttta
      541 aaatctaatc ctgctaaatc aatagatttt aaatttcata ctggtgtaac tactgacatt
      601 attcaaggtc ttaaagaaag aaaatatgat attgcatttt gttcgaaact tgaaaaagaa
      661 aagggaattg aatttatacc tgttgccaaa caagatttag tactaattgt accatatagt
      721 catccactag ctgcaaagga tactatagat ttaaaagaaa ccatacctta tcctcaaata
      781 gtatttaatc agagaagtgg tcttagatat ataattgatg atatgtttaa aaaaataaat
      841 caacagccta atatagtcta tgaagtagag gaagaccaag taattgctgg attagttgca
      901 aaaaatttcg gaattgctgt agtcccaaat atgaatatgc ttagctttac aaaagtaaaa
      961 gtaattcaga ttattcaccc aagttgggaa agaaactttt acttagcatt tataaaagat
     1021 agatatttgc ctcctgcaat taaaaacttt aaaaactttg tcattaaaaa cgctcaatta
     1081 taatttatta ataaaggggt tatttttaaa tatcctcttt tattcttttt ataaaataaa
     1141 aaaatctata cttttaaaag tatagattcc taactgacag tcggcatccg agtctcagta
     1201 attatcgtta tacaacagca actggattca gttcctgaat aactaggtta cttacattat
     1261 atcagatttt ttaaaatttg tcaattgtat ctaacgcatt ttttcaagcc atggtccaac
     1321 aggctctaca ataggaactt ctttaaatcc ccaatttctt atatcattca cactcgttgg
     1381 agtatatatt ttgtttctga cagatttgaa tattttgttt ttttctccat attgcataca
     1441 ctttggatta gcttctatta accctcttct tgctctgcta agaggattac ataaaatatc
     1501 tgctatttct aaaccttctc tatagtcagc atcttctttt ttggcaatta taaaaccctt
     1561 aaccttatct cttaattctt catctatatc taagtatatt gttccattat taaatacatc
     1621 aaaaaacgcc ttttgtactt ttaagtttga gacatcatcc cttgactcta taactattcg
     1681 tgcagattct acagtttcat tatttacaaa tgaataaaaa ttctgaagaa catgtataaa
     1741 agccacaaca tacggttcct ttgaaggtgc ataatatttc tttaatctag ctttatctac
     1801 tgttactgat attatagtac actctaatct agatagaaat aaaggcaact catcatagaa
     1861 taacttaatc tgtgaatcat caaacttctc aaatgctctt gattttcttg atatatctgt
     1921 caaatgtatt cctatgttac tggttccaaa acaatactct ttaaatgcat ccatttcttt
     1981 cctcaaagaa catacataat cgccttgact tactgcatat ttatattcaa aaattactcc
     2041 tgtcaatgta aatgggtctt catgatttat atcagctctt ccactttcat caataaacat
     2101 tatatatttt ttctttataa tcttactcac cctcttaaca tatgatgata gcatctatat
     2161 tcattttata tgagttttaa aaatattcca agatttttat ataacttaaa cttaatttta
     2221 tactaaaaaa ttgagatgta aaacaatcaa gcttttattg attgaatttc acatctcaat
     2281 tataattatt tttcaatata tatttttact attttgcaag aatatccatt aatttatcca
     2341 ttactatata agatactccg cctcctattt gataaggagt ttcaactttt ttatttgcaa
     2401 atactctttg ttgctctgat gataatcctg aattatccat taaaactatt ggtgaatgta
     2461 attttacagc taatggtcca gctgataaag catctactaa agcatctgat ttactaacta
     2521 atacagcctg taaatcagta tctccataga atttttctat cactttggca ttagtatcta
     2581 ctctactatc tccatatatt ctatttcctg aaatatcttc agtagttata tcattcattt
     2641 ttgctattac tgaatcgtta attgcacttg gtccacctat aaagtatgca ttttctaatt
     2701 ttctttctgt tagccattta taagattctt ctgtaattcc atctttattg actaatatta
     2761 taggttgttt ttcttcccca gctttagaag ctacagataa agcatctgcc tccccatttc
     2821 cagaaaccat atatgcagtt tttatttctt ctcgatttcc aagttcttca gctattcttg
     2881 tagcagtttg atatctatct tgacctgcta ttctctttgt ttctgtgttg aaagtagttt
     2941 ttatttcttg ctctaattta gaacttattg cattttctcc acctatcaag attactgttt
     3001 taggtgctaa taacttcatt tcttcaacag tttcagttgg taaattagct gtattagtaa
     3061 gtagtattgt tgagtcatag ctacttgcta aaggagttgc tgttatacca tcagctattg
     3121 cattaccatt tactaatatt acagtatctg aactatcagc attccagttt ccaactgaac
     3181 ttatcttttt agctgtatca aatctatttg cacctataat tgtatcccat gtaggattta
     3241 tagttccatc ttcattaaaa gtataatctt tcccatctat tttttgagaa ccagttagca
     3301 tctttccatc tttgtctaaa tagtaagact taccatctat ttgttgccat cctgtctgca
     3361 ttattcctgt tttgtcaaag taatacttat taccatctat ttcttgccat cctgttactg
     3421 ctaaaccatc agatcctaga tagtatttct ttccattctt ttcttgccat cctgtttgca
     3481 ttactccatc tgagttaaag taataagttt ttccgtctat tactttccat ccagatactc
     3541 ttgctccatc agttcctaaa taatattttt tgttattaat tgttttccat cctgtttgca
     3601 ttactcctga agcatcaaag taatacattt ttccatttac ttttagccaa ctattctttt
     3661 ggtatgttcc atcagatttt acataatact tcttaccatt ttctgttttc catcctgttt
     3721 gatttagatt tatcttatat tttgccgcta tctttacata gctgtcgtta ctataatcta
     3781 taattacatc tgaacctaca ggaacttggt caaataacca tctaacatct ttattgtgca
     3841 ttcttataca tccaccagaa atatgctttc ctatagaact ctcattatta tttccatgta
     3901 ttccatatgt atctccataa gttcctttta atgctaatcc catccatcta tctcctaatg
     3961 gatttcttgg acttcctcca ggtatattac ctttataata tggtctattt tttatcttgt
     4021 taactacttt tgttttccct gttggtgttt ctgacccctt tttacctgtt gcaactctaa
     4081 attcttttac aagtttatta tttacaaaat atcctagagt attttttctt gaatttacta
     4141 taatcacatg tttactggct gcgcttgatt ctggtacgcc acatgcaaat actaaaacta
     4201 aagtcagtgc tagtactaaa tactttttta gacttttttt cactataact tccccctctt
     4261 ttagaaacat tactacatgt gtctaattat aacataaaat taccaatacc ttatatttac
     4321 aaatttgtta catttataca tataagtatg tattctactc ttcataccca ttaggattca
     4381 tactttgcca tctccatgaa tcttcacaca tctcttcaag ttcatactta gctttccatc
     4441 caagctcttt ttctgccttt gaagaatctg cataacacat ggctacatct ccagcccttc
     4501 ttcctacaat cttatatggt atttcttttc cacttgcttt agaaaatgct tttactaaat
     4561 caagaacact ataccccttt ccagttccta aattataaac aacaagtcct ggattttctt
     4621 ctaatttttg taatgctttt acatgaccag cagctaaatc taatacatgt atatagtctc
     4681 taactccagt tccatcatgt gttggatagt cattaccata aacactcaat tccttcaatt
     4741 tacctactgc tatttttgtt atataaggca ttagattgct cggaactcca tttggttctt
     4801 cacctattct accactttta tgagcaccga ctggattaaa gtatcttaaa agagctatat
     4861 ccaatgactt atctgctttt gatatatcta ctaacatctg ctctatcata agtttagttc
     4921 ttccatatgg attggttaca gaaagtggaa aatcctctaa tataggacaa gtatgtgggt
     4981 ctccatatac agtagctgaa gaactaaata caaacttttt aacaccatat tctctcatta
     5041 attcaaataa atttaaggta cttattagat tattgctgta atattcaatt ggtttttcta
     5101 ctgattcacc aactgctttt aaagctgcaa agtgtataat tgattcaata ttattttctt
     5161 taaatacaac atgcatttca tctttatttc tgatatcaat attataaaat tttactggtt
     5221 ttttagataa ttccttgatt ctatcaacca ctattgagtt gctattactt aagttatcaa
     5281 ctataacaac ttcataacct gattctaaaa gttctattgc tgtatggctt cctatatatc
     5341 ctgctcctcc agctactaaa actgccattg taattacctc ttttctaata tttatttttc
     5401 acttactttt tttctcatat agtccattaa atcatctttt aaattttctc tctttagagc
     5461 aaagtctatt gttgcctcta aaaaaccaat tttatctccc acatcatatc ttctaccttc
     5521 aaagttataa gcataaatag cctcttgttt tccaagagtt tgaagtgcat ctgtcagttg
     5581 tatctctcca cctttaccag gtgtctgttt ctctaatatg ctaaatattg caggagttat
     5641 tatatatctt ccaagtatag ctatatcaga tggagcttct tcaattgcag gtttttcaac
     5701 catatcttta actttatata ctctatcttc gatatgtttt acatccaata taccatattt
     5761 attagtatct tctttagcta tcttttgaac acctaataca gttgttttat actcattata
     5821 agtatctatt aattgtttta agcacggaac atctgaatct actatatcat cacctaataa
     5881 tacggcaaaa ggttcatcac ctataaaact ttttgcacag taaattgcat gtccaagacc
     5941 ttttggctct ttttgtctta tataatgaat atttaccata tttgatatgt ctcttaccat
     6001 ttctaacatc tctgtcttac ctttttgctc tagttctaac tctaattcaa ctgatttatc
     6061 aaaatgatct tctatagatt ttttatttct acctgttatt ataagaattt cttcaattcc
     6121 cgattctata gcttcttcta ttatatactg taaagttggt ttatctacta taggaagcat
     6181 ttctttgggc tgtgattttg ttgctggtaa aaatctagtt cctagccctg ctgctggtat
     6241 tattgctttt ttaactgtca tctgcatttt ttcacccttc ttcgtaaatt ttctattgta
     6301 ccaaattaat ataccatatt tatacttttt cttcaaactc tatattatcc caatatttaa
     6361 tgctataaga taatataagg tagatgaaat ttcatctacc tttagtttta taagttaata
     6421 ttgtattgtt tatattttat ccaaaactta tacaggtact aaatttcatc taagatgcct
     6481 tttatatcac tatctatatc ttcatctaga taagaatcta aatcttctaa gtcaacttct
     6541 agttcttgat atgtcatatc aacactgaca tcctcatcta tatccatttc tgatattaat
     6601 tctcctaaaa tactatttcc atcagaatct aaaccactat ctatatcttc atctatatag
     6661 ttttcattta aaatttcatc taaatcaatc tcgtaataaa cttcattatt tttttcatcc
     6721 cctgagtcca atcctaagaa ctcatagtta aaagtccaaa cattgtattt acttgacata
     6781 ctttattcct cctgtgaaaa acatattttc atctagttta ttggttccat aatcaacaga
     6841 gttatatatt ttttcaatta tgtcttttct tctttctttt gtactattat ttaatgtcaa
     6901 ttgtttaaat acatatgtat ttgttattct ctcgaataat atttctttta tactatcatc
     6961 atcaatattt ctatagtttt tccacaataa ctcaaataac ttaaacattt ccctactagt
     7021 ttcatctaca taataaggat acatattttc tactaaatgg tttaatcttg atatggcttg
     7081 ttttaaaaat acatcaagac ttgcactttc actttctttt aacattctat aattgtattt
     7141 cttcttatct tctcctgaaa ttacaaagat gatattgtct aaactttttt caacttcatt
     7201 taaataactg cttatattat atgataaatc agacaataga tttttaatct gcttaaacac
     7261 ttgtacatca agtttagtat tatacttttc aaacaaatct atttcttgat tttctaatat
     7321 tcttggcata tatgctgaca ataaatctaa attgtttctt tgttcttgta tttgcttata
     7381 aagtgaattt ataacttcta ttcttatttc atcatttaac tctttttcta aaatcttatc
     7441 aaattcagat ttcatatagt aaatatttcc aaagaaatta aatattatca tatcattcat
     7501 agctttagac caattcttat atatcacttc aatagaattt tctttaagta gaggttgctt
     7561 tgcatatata aatacatctt gcataagttt ttgaaccata cttttactta agtttaaaag
     7621 tactttttga ttattaaatt ttatttccgt ttctacaaaa ttagaatttt cgttttcaag
     7681 ttctttatta attacaatgt cttttatatc actatttttt atagatatga tttctttttg
     7741 aaaagacttt agaatagtca agctatcgaa atcttcttga ataaaaaatg gagcatgttt
     7801 taatgtatat cccactattt cattttctat atcttttagg tcatctattt gcataatttc
     7861 tctattttta tcatttatat gtaggttaaa tacattttta ttatcatata atattatatc
     7921 ttctcctaaa tttaacttag aacaattatc tttcgctgac tttacaatag tcttcttaga
     7981 tttcttattt ataaaaatta attggtcttg atattttact aaaagcatgt ttgtgttttt
     8041 ataatcaaat ccatctatat ttcctgatat cttgcagaat actatttcac aatctttagg
     8101 aagatttcct attttttggt tatctttaac tctctcattt aaatcagatg ggactacact
     8161 atctatattg ttattaaata tagatacaga ccttactata atcccctctc tttccatatg
     8221 aaagtatccc ttcaatgaca ctcttttatc ttctaaaatg tatctttcta tatcatcaaa
     8281 atttatttca aatctatttt tcccactgat aaatataaac ttctcattat ctttattaat
     8341 tccacccata taaaacttat tgtcatctaa acttaatata gtcatattat cctttagata
     8401 gaatataaat tctctttgac ttttgcagat taaatcatat tcatccaaag ttattttcat
     8461 agtcaaattt gctgtaacat ttttacttga attttcactt tttatgtaaa aaggcaagtt
     8521 atttccatag acaattattt gagaatcttc taaaactgcc tctaattttt ctttattgtc
     8581 tgaaaaacta tatatatatt ttacacctgt atcctcatta acttcatctg atttgctgat
     8641 tattatattc cctagatttg ttatatgaag ctttttatat tcttttgagt atctacttat
     8701 gccactaagc tcaaacccat ctgaaagctc atatatgatt ttttctccta atttcaacat
     8761 accttcacca cctaaacaga attataagta ttttataaag gtgaataaaa tgtctcaaaa
     8821 caaataatat tttttttaat actttttatt ctgagacata gtttctcctt ttttataatg
     8881 ctctaagcac cttcaactat ttcttcataa agtttatcta aatgtttaac atattcacga
     8941 tatccagatt tgttctttaa ctcacttctt aaatcttcat atttttgttt aagttcttca
     9001 gcactcaaag aaggagctga ttcttttaat tcttctaact tataatcata gtacttatca
     9061 tacatagcta tatatgtatc aatagacttc ttagctagtt cattacattt ttcttgtgct
     9121 tctccatatt gagaataatc acctattttt attttatagt atccattttc ttgaattatt
     9181 gttgaagaaa tacctttagc ttcatattct tttgctgttt cttttgctgt tttttcaact
     9241 ttaagtgaac ctgcataaac agtgtaatat acacctttac catctttata tgcttccatt
     9301 tcttttgctt tcttttcagc ttgtgctttt tcttgagcct ctttttcagc ttttgccttt
     9361 tcttctgctt cttttttctc tttttcagct tgtgcttttt tctcttcttc agttaattta
     9421 ttgttagctt gttgttgagt tgatgacttt ggaatattct ctttatcatt tttcttattg
     9481 ttgcctactg ttataaaata tcctacacca acaataacca ctataactac tgctataata
     9541 gtttctttta atctactctt cttagaaaca acctcttcat catctaaaga atcttcttta
     9601 tctgatacat aatttttcat ttctttcttg ttttctctat atttgtcctt agaagaattt
     9661 ttagattttt tattactatt ttctttcttt ttaataggta ttactactga tttttcattt
     9721 tctaattttt cttcttttat ttcttttgct tttttatctt ctaagttttt actttctttt
     9781 tcaagcttcg attcattatt tgaagatact ttttcatcta agttattatt tattggtttt
     9841 tctattttta cactacgttc ttcttctata tgttgacttg gttcttcttg ttttatttct
     9901 ttcaattcat tttgctctat ttcttctgat ttcacttgat gattttcaac tggtttatct
     9961 aaaattatat ctggagaaat atcatttaaa ttctcttctt cttgttcagc ttcttcattg
    10021 tcttttttaa taaatccaaa taatttagcc ttctttttac tctttttatt ttctggttca
    10081 attatatctt ctttattgct gtcgtactct ttatctgtag tatctttatc ttctacaacg
    10141 tcacctatat cttgattatt actatctact tcttctttag tcccttcttc agcatcatct
    10201 tctattgaaa tactacttct aaagcaaata tctttatctg cattttttat catgtaaatg
    10261 tattcttcaa tatcgatatc tttatttctt ctagttcttt ttgatgcaat ttcatgattt
    10321 tctaattcac tatagttatc ttttattaga ttctgtgctt tattccatgt atcttcacta
    10381 atttctgttt tatttttaat tacatagtcc acaaattcct taaaattttc aggaccaaat
    10441 tctccaatta tagtcagtct cattgcacct tctataaggt ctctatttct tctctttaaa
    10501 aattctaata caactgttgc acaagcaaaa tctttaataa tttctttgcc aaaaccatat
    10561 gcttcaacag aatgaagttc ttctaatcca cataacttct tatattcatt gtgtattgtt
    10621 tgagatatac tttcattatt taagctattt aacttatcta taaacttcga ttctatatca
    10681 tttgattttg attcatgttt tacttttgaa tttttaattt catttagtag catgtcattt
    10741 tctgaattat ctttacctaa ttgcaaatcc tcaatttcag ttgcttttat ttctactttg
    10801 ttttgttcgt tattctcttc cacattatta ggtaccacat ctttattact agctaatatt
    10861 ttgtttacac agaatataat ttctcttttt aattctcttt tcgcatctgc ttgattttta
    10921 agaaataaat ctccacattc tttcttttct aaacttattt ttctaagaga tatattaaga
    10981 aggtctttat ataggtttaa aaccttataa tccttttcta aacgtcttct tctctctgat
    11041 agttccattt tatcataagc taaaaaacta tttaaatcat tggcttttag agagtctaat
    11101 ccctctttca tagccttatt tacatacttt ctacattttg ctgtagttaa ctctaaatct
    11161 ttttctaatt catctatttc aggtatgtac ttatctacta tccatttgcc aataagtggt
    11221 tctgtatttc ttgctaatgt cttataaaac aatatattaa attttaaaag ctctgcatat
    11281 tttgcttcat taaattctac tttttcaaat ttcttttcca aaacaccatc tagcttttca
    11341 tttattttag actcttgttt taaaaaactt gctgtgacat ttaaaacagt gtttcttatt
    11401 atattatttt cactcaaaac cttcactcct taaatcaatt attttattgt aatatttaca
    11461 ttgtgcaaag ttgctaattt gattatgata tctaaataat tatttactaa atttgctaca
    11521 tcttatatca tatcatatta tttacatcat tttacttttt ctttttatat ctatcttata
    11581 ttataccatt tttaatatct gtactaactt atattacaat attcctcaaa attttaaaag
    11641 tagattagac aaatttacaa ataaaaaaac atcagtttaa actgatgttt ttgactatcg
    11701 aaataattaa gcaagtctat aagcggagtt ctgtattaga taatcatctt tctaggccta
    11761 ttgtcaccaa taggctcaag cgacctacct accggagagt acgagcagca ctctttattc
    11821 ctatcttggt cttgctccag gtggggttta catagccaac tagtcgccta gctgctggtg
    11881 agctcttacc tcgcctttcc atccttacca caatagtggc ggtttatttc tgttgcacta
    11941 tccttaggat cgctcccact aggcgttacc tagcaccctg ctctatggag ctccgacttt
    12001 cctcatgtaa gattacatgc gattatctgg cttacttaat atttatttta cttagttatg
    12061 ttaacacaaa ttcttttaca tgtcaaagta atattatact aaaaatttaa atttattcaa
    12121 aaatagtgta atatagatat ataaactaca tttaactcga ggtgatctat tgaaaaaatt
    12181 atatagaata gtaattaaca ttatactagt attagtaata ctttacagtg gatttaatat
    12241 atactcaaaa cttaccaaat acaatcatga tactaaaata tcctcagaac ttcaaaaaaa
    12301 agaatataaa aaagaggatt tatcaaagat taacagtgat tttaaatttt ggctttctgt
    12361 tgagaataca aatataaatt atccagttgt acaatctaaa gataattctt actatcttga
    12421 caaagatttt tataaaaaag attctatttc tggtacactg tttatggatt atagaaataa
    12481 atctattgat gacaaaaata tcataatata tggacataat atgaaaaata aaactatgtt
    12541 caacaaccta aataagttta aagatgctga cttctttaaa aaaaataata aaataaaaat
    12601 aaccttgaat ggaaaagaat ttttatatga tgttttttct gcttatattg tagaatctga
    12661 ttatgattat ctaaaaacaa attttaacaa tgaatctgat tatcaaaatt acataaatga
    12721 tattacttct aaatcattgt ataaatctcc tattaaggta aatagcaatg ataaaatagt
    12781 tacactttct acatgtacct atgaatttga tgatgccaga atggtgattc atggtagatt
    12841 gatttaaaac tcacagacac agtttaaaac aataatttta aactgtgtca tcttttatac
    12901 ctataatttg taaattagac cactaaaagt atttaataca aaataacaaa tacatcaaac
    12961 aataaaaaac aataaaaaaa gactatgcta aaaacatagt ctttactgga gctggtgata
    13021 ggaatcgaac ctacaacctg ctgattacaa gtcagctgct ctaccgttga gccacaccag
    13081 cacacctatt ttattgtaaa ttagcaaaac taatttttaa aagtta
//
LOCUS       DAMJVI010000099        12774 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.104, whole genome shotgun sequence.
ACCESSION   DAMJVI010000099 DAMJVI010000000
VERSION     DAMJVI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.104"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            123..1613
                     /locus_tag="PC545_002939"
     CDS             123..1613
                     /locus_tag="PC545_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2457692.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEEDEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEDKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMEKGEAFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            1893..3389
                     /locus_tag="PC545_002940"
     CDS             1893..3389
                     /locus_tag="PC545_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2457693.1"
                     /translation="MFEFETQLKRLKHEVLTAVARLSIEENLTKENIEKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVSLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFVDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKMFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKSLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSVQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEDVNLER"
     gene            3523..4437
                     /locus_tag="PC545_002941"
     CDS             3523..4437
                     /locus_tag="PC545_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2457694.1"
                     /translation="MRKIVLKDILFLVTTLSIIVILLLVTNITTNSKIKSTNESYLKS
                     DKNDVSQNNIQSSTKNTAFKTTILSTGKSDCILVEIGNKVIMIDTGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKDNMNIVINSAEINIHPASKSKYESSNDYSIITNISYGAHKFLFAGDAEEE
                     RLSEFINGNTLKYDFVKMPHHGRYDKLTETFLESISPQYAVITCSEKKEPEEGVLKIL
                     ERLNIKTFLTSNGEVVINSDGKTLSVNQ"
     gene            4555..4746
                     /locus_tag="PC545_002942"
     CDS             4555..4746
                     /locus_tag="PC545_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457695.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            4969..5319
                     /locus_tag="PC545_002943"
     CDS             4969..5319
                     /locus_tag="PC545_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457696.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGNVNEAEEILFDKAKSVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            5581..7086
                     /locus_tag="PC545_002944"
     CDS             5581..7086
                     /locus_tag="PC545_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDF2457697.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHENKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAVV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            7476..8573
                     /gene="dinB"
                     /locus_tag="PC545_002945"
     CDS             7476..8573
                     /gene="dinB"
                     /locus_tag="PC545_002945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDF2457698.1"
                     /translation="MKHYRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLRK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLDIDEEETRNILDELCEEVCH
                     RLKNSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRSGVDNLLRNVDVNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            8836..9972
                     /locus_tag="PC545_002946"
     CDS             8836..9972
                     /locus_tag="PC545_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457699.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSDEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDIDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            9965..11512
                     /locus_tag="PC545_002947"
     CDS             9965..11512
                     /locus_tag="PC545_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDF2457700.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKFVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVLVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVKKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMKHAPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVMNMLSGKINEETMRELNYQVDELGKSPEEVAHSF
                     LVKEGLV"
     gene            11846..12712
                     /locus_tag="PC545_002948"
     CDS             11846..12712
                     /locus_tag="PC545_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDF2457701.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVLR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRIGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLVGVKLGFPVIIESIVPIIISAFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEQSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
BASE COUNT         4955 a         1336 c         2325 g         4158 t
ORIGIN      
        1 aaacactata tatattcgat aaaataaaat ttaaacatat aatattttat gtatatatta
       61 ataaaaggac atattgtgtt ataatggtat cgtataaaat catttacggg ggtggtatta
      121 aaatgtttca atttgaaaca caaatacaca aattaagaca tgatgtttta actgcaatag
      181 ctagacttgg aaaagaagat aaccttacca gagaagcaat ggataatatt cagtatgaaa
      241 taataaaagg tgagaaacct acatacagat gctgtgttta taaagaaaga gctataatat
      301 ctgagagagt aaatgtaagt atggggttac aaccaggtca agataagata gatttagata
      361 caatggatat agaagaagat gaacaaatta tatatatttt ggaagctgca tgtgacagtt
      421 gtccaataaa tgaatttaca gtaacagata tttgtagagg atgtctagca catagatgta
      481 aagaagcttg taaatttgga gctataacac atgtcggagg aatggcctat ataaatcatg
      541 aattatgtaa ggcttgtgga atgtgtaaaa aagcttgtca atatgatgcc atctcagagg
      601 ttgttagacc atgtaagagt gtttgtccaa ctaatgcctt aggatttgat agagaaaata
      661 tgaaagctat gatacatgag gataaatgtt taaactgtgg agcatgtatg tcagcatgtc
      721 catttggagc aatatctgat aagagtttga tagctcctgt agctagaaag ttagttcaaa
      781 aagagaaaat gtatgcggtg gtagctccag ctataacagg tcaagttgaa gcaaatgtta
      841 catatggtca aataaaaaat gctataaaat cactaggatt tgttgatgta ttagaagcag
      901 cttgtggagc agatgcagtt acagttcatg aaagcttaga atttgctgaa agaatggaaa
      961 agggagaagc ttttatgaca aattcttgtt gtccaggatt tgttggttat atagaaaaaa
     1021 catacccaaa tcatgtagga aaaatatctt ctacagtgtc tcctatgata gctagtggta
     1081 gatttataaa aagtcatgaa ccagatgcta aagttgtatt cataggacca tgtacagcca
     1141 aaaagagtga ggcagctaag aaagaattaa aaggagctgt tgattatgta cttacttttg
     1201 aagaaataat ggcattattt gaggcatttg atatagactt ggctaagtgt tcaaatgaag
     1261 atatagatga tgcatcaatt tttggaagag gttttggagc tgctggtggt cttacaaaag
     1321 ctatagaaaa ttatcttgct gaaaaaggta ttggtgttaa ttttgaacct gtaaagattt
     1381 ctggttcaag agaaatcaag aaaactatga ctatggctaa tttaggaaag ctagaaggta
     1441 attttataga aggaatgatg tgtgaaggtg gttgtataaa tggttctggt aaattcttat
     1501 caggtgcaaa ggttaaagcc acttttgata gaaaaaattc tcaatcaact aagaaaagtg
     1561 tcttagcaaa tacaaagata aaagatttta gtaatataaa tttagaaaaa taaagataat
     1621 acaaagcaac aaaaatacat attgttaata gagttgttct aagttgaggt ttaacttttg
     1681 gacaactcta tttttattta tatatttaat tattgaacaa ctaaaataaa aattatatac
     1741 acttaaaata aaaattacat atataatatt ttgtatataa cttaacaaag tatatgccta
     1801 tgttataata atttatagaa ataaataaaa aatacaatat acataatagt agaaatatag
     1861 caaaaaggtg tgatttatag ggaggattac ttatgtttga atttgaaacg caactaaaaa
     1921 ggttaaaaca tgaagtttta acagcagtag caagactatc tattgaagaa aatttaacaa
     1981 aagaaaatat agaaaaaatt ccatatgaaa taattaaagg agaaaaggcg agatatagat
     2041 gttgtgttta taaagagaga gaagtagttc ttgaaagggc gcaacttgca ttaagtttaa
     2101 ctccaaatag taaatatgga gatataaatc cagattcagt tagtttggac acagatgagc
     2161 aaattatata tatagttgaa gcagcatgtg atagatgtcc aataaatgaa tttacagtta
     2221 cagagctttg tagagggtgt ttggctcata gatgtaagga agcatgtaaa tttggggcaa
     2281 tttcttatat aaatgggaga gcatatataa atcatgattt atgtaaggct tgtggaatgt
     2341 gcaaaagttc atgtcaatat gatgctatat cagaagttgt aagaccttgt aaaagcgttt
     2401 gtccaacagg agctttggat tttaatagaa acactatgaa agcaatgata catgaagata
     2461 attgtataaa ttgtggagca tgtatgtcag catgtccatt tggagcaata tctgataaga
     2521 gtttgatagc acctgttgca aaaaaacttg caaagaaaga aaatatgtat gcaatagtag
     2581 ctccagctat aacaggtcaa atagatagta atattacata tggtcagtta aaaaatgcta
     2641 taaaatcact gggatttgta gatatgatag aagcttcttg tggagcagat gcagttacag
     2701 ttcatgaaag caatgaactt attgaaagaa taaaatcagg agataattac atgacaaatt
     2761 catgttgtcc gggatttttt aagtatatag agaagatgtt cccagatgaa aaagatagaa
     2821 tatcatctac agtgtctcct atggtagcaa caggaagatt agtaaaaaaa atagattctg
     2881 atgctaaagt tgtatttata ggtccatgta cagctaaaaa gagtgaggca acagagaaaa
     2941 gtttaaaaga ttcagtagat tatgtactta cttttgaaga actaatggca ttatttgaag
     3001 catttaatat agatttatca aaatgttcaa atgaagatat aaatgatggt tcgatttttg
     3061 gaaggggatt tggagcatct ggtggacttg ccaaatcagt acagaactat attgctgaaa
     3121 aggggcttag ttttgatttt gaacctataa aagtatctgg tccaagacaa attaaaaaga
     3181 caatgacaat ggctaaattg ggaagattag aaggtaattt tatagaagga atgatgtgtg
     3241 aagaaggttg tattaatgga gcaggtaagt ttttatcagg tgtaaaagct aaaaatactt
     3301 ttgatagaaa aaatggacaa tctactaaaa agagtgtttt atctaatgat aagattaaag
     3361 aatttgaaga tgtaaattta gaaagataat tgttttggta cagtcatttt tttatcatgc
     3421 cacaaatatg cctaaaattt gcccttttta tatgtgtatt ttacagagtt aaggttgcta
     3481 tactataaat atgtaataaa taaaatttat aggagctttt ggatgaggaa aatagtatta
     3541 aaagatatcc tgtttttagt tactacatta tctataatag taatattatt gcttgtaaca
     3601 aatataacta ctaatagtaa gataaaatca acgaatgaat cttatttaaa aagtgacaaa
     3661 aatgatgtga gtcaaaataa tattcagagt agcacaaaaa atactgcatt taagactact
     3721 atattaagta ctggaaagtc tgactgtata ttggttgaaa taggtaacaa agtcataatg
     3781 attgatacag gagaagataa aaatggaaaa caaatagtgg ataggttaaa agaaaaagga
     3841 ataaatacac ttgactattt aattttaact catttagata aggaccatat aggtggagta
     3901 gatagtgtgt tatctagtgt taaagtaaag aatattatac aagcaaatta taagaaagat
     3961 agtaagcaat atgatgaata tattgattca cttaaaaaag ctgatataga accagtgcta
     4021 ctaaaagata atatgaatat tgtaatcaat agtgcagaaa taaacataca tccggcttca
     4081 aaaagcaagt acgagagttc aaatgattat tcaataatca ctaatataag ctatggagca
     4141 cataagtttt tatttgcagg agatgcagag gaagaaagat tgtcagagtt tataaatgga
     4201 aatactttaa aatatgattt tgttaaaatg cctcatcatg gaagatatga caaattaact
     4261 gaaacatttt tagagtctat ttcaccccaa tatgctgtta ttacatgttc agagaaaaaa
     4321 gaaccagagg aaggtgtttt aaaaatattg gaaagattga atataaaaac atttttgact
     4381 tctaatggag aggtagttat caatagtgat ggtaagacat tgagtgtgaa tcaataattt
     4441 ttttaaaaat atctaaaaaa atttaaaaaa atcattagca aatattaccc cttaaacata
     4501 gtatatttta ggagctgaaa ctccacatac aaaatgacta ggaggtaaca tattatgttg
     4561 gatagatttt ttggtgaagg cggatttggt ggatgtgctt ggtggataat aatattattc
     4621 ttcttattct tagcattcca agattgttgg gctgaaatgg acttatgtgc atggatacct
     4681 ttcttaatat tattattaat actttgtgaa tgtggtgacg tttttgatgg aggatgtgga
     4741 tgctaaaaaa atcaatttag atgatttaat aaagcttatg gtgggtataa ataaaatacc
     4801 aaaccaatca caatctagtg tttgaaggta gtcggaaatc ccccgctacc ttcttttttt
     4861 tattgttaaa atttattttt gaattatttc atttacatat aataattttt acgataaagt
     4921 ctgatataat taaaaagaaa ataaaattta tttataggag atgtaaaaat ggctatagtg
     4981 gataagattt tcaatgagat aaaacttatg gttgcaagac ttttattggg aaaaaaatac
     5041 agtgaatatg aagaaaaaga tttagtgtat aatacagaag aagaagttat acttataact
     5101 ttaaaaagat tagtttttca gggaaatgta aacgaagcag aagaaatttt atttgataaa
     5161 gctaagagtg taaatagtga aaatatgcag tatatagcaa tagaatttta tactatgctt
     5221 atggaaaaaa ctgatgaaga attagaagct atgaattttt ctaaacaaga agtttatcaa
     5281 ggtatagaag atattagaaa agtattgaat ttagaataaa agatatatga aaatattgaa
     5341 ataaattaat gctaaggtga gaatattata cttatatata aaaatatagt attcttattt
     5401 tattagttat attaggtata tcttatttac cataaaacta tcttgaaaaa aattaatctt
     5461 aattgtataa tattactttg agtgaaaaag tattaacatt tttttataag atgttttact
     5521 aaaaaaatat atataaatat actgttatca ataagaaatt taatttagtg aggtgttaag
     5581 atgaaagtag gatttgacca caaaaaatat ttagaagagc agtctaaata tatcttagaa
     5641 agagtaaata attttgacaa actttattta gagtttggag gaaaattgat gggagacctt
     5701 catgcaaaaa gagtacttcc aggatttgat gaaaatgcaa agataaaagt attacaacat
     5761 atgaaagaaa aagttgaagt tgtaatttgt gtctatgcag gagatataga aagaaacaaa
     5821 ataagaggag actttggtat cacttatgat atggaagttt taagattaat agatgattta
     5881 agaggttatg aactagatgt aaatagtgta gtaattacta gatttgaagg acaacctgct
     5941 acaactgtat ttataaataa attagaacgt agaggaatta aagtttataa gcattatcca
     6001 acaaagggat atccgtcaga tgttgagact atagttagtg atgagggata tggagcaaat
     6061 gaatatatag aaactacaaa gccaatagta gtagtcacag caccaggacc taatagtgga
     6121 aaacttggta cttgtctaag tcagttgtat catgaaaata aaagaggtaa tgtagttgga
     6181 tattctaagt ttgagacatt cccagtttgg aatgtacctg taaaacatcc tttgaatata
     6241 gcttatgaag cagctacagt tgatttaaaa gatgtgaata tgatagattc attccacctt
     6301 gaaacgtatg gtgaaatgtc agtaaactac aatagagatt tggaattatt tccagtgctt
     6361 aaaaaaataa tagaaaagat aactggaaaa gaatcaattt tccaatctcc aactgatatg
     6421 ggtgttaata gagttggctt tggaataata gatgatgaag taataagaaa agcttctata
     6481 gaagaaataa taagaagata ctttaaaact gcctgtgagt ataaaaaggg acaagttgat
     6541 aaaggtgctt atgatagaat gaaaatgatt atggaagagc ttaacttaaa gccagaagat
     6601 agaaatgttg tattacctgc tagaaactac agtaataaat tgaaagaaag tgctgacaag
     6661 aatgatactt gtcctgtagt tgcattagaa ttagaagatg gtacaatatt aactggtaag
     6721 ggttctgatt taatgaatgg aactgcagca gtagtattaa atgccattaa acatttagcc
     6781 aatatatctg atgatatgca cttaatatca cctgtaatat tagaacctat cataaactta
     6841 aaaacaaaga ctttagctag tagaaatgtt tcacttagtt gccaagaggt gttaactgca
     6901 cttagtattt gtgctgtaac taatccaact gcacaatttg ctatggaaaa gttagcatta
     6961 ttaaaagggt gtcaagctca ttctactact atattaaata gagatgatga acaaacattt
     7021 agaaagcttg gtatagatgt aacttgtgac ccagaatatc cttcacaaaa tctttactat
     7081 agttaaaatt atataaaaaa atatgggctg ttttaaagta atagtttgag cagtccatta
     7141 caattataat aaaatcagta ggctgtcttg cataagacag tcttttgttt ttaaaacatc
     7201 ccataaatta aatatatata ctgttatcca taaggaattt taaatttagt tccttggaat
     7261 acctattgta aagcttttat atagtttttg caagtgtttt taaggatttt ttataaatta
     7321 tattgaagtt aatggattat agatggtttt atttatggaa aatatgaata tataaagaaa
     7381 ataatgaaaa aataccaaaa agaaacataa gtttggtata attaaaaagt aatataaata
     7441 aaattaatct atttatactt taaaaggtga taataatgaa acattataga aaaataattc
     7501 atatagatat ggacgctttt tatgcatcta tagaacaaag agataataaa aaattaaaag
     7561 gaagacctgt tattgtcggt ggaaatccac aaagccgagg tgtggttgca acgtgttctt
     7621 atgaagctag aaaatttggt atccattcag ctatgccatc agctgtagct tacaataggt
     7681 gtccatatgc agtttttgta agacctcgat ttgatgttta taagagtgtg tcagagcaaa
     7741 taagagatat attttataga tacacggatt tagttgaacc attatcttta gatgaagctt
     7801 acttagatgt aacaaaaaat aaaaaaaata ttgattcatc aattgagata gcaaagcaaa
     7861 taaaaaaaga tatatttaga gaagttggtc ttacttcttc agctggtgtg tcatacaata
     7921 agtttcttgc taagattgca tcagacttaa gaaagccaaa tgggttaact gtaataaccg
     7981 aagaaaatgc ccaggacttt ttagataaat tacctgtgaa taaatttttt ggtgttggaa
     8041 aagttacttc aaatactctt aaaaatttag gtataaaaac tggatatgat ttaaggtgtt
     8101 taaatttatt tgaacttgaa aatatattca aaaaaagagg ttatgaatta tataaatttg
     8161 ctagaggaat agacgataga ccagtggaac caaatagggt tagaaaatct gttggggctg
     8221 agactacatt aagtcataat ttggatattg atgaagaaga aacacgcaac attcttgatg
     8281 aactttgtga agaagtatgt catagattaa aaaactctga gaagtttgga aaaacattaa
     8341 cattaaaaat taaatatgag gactttacaa agataactag aagccttagt ttagaacact
     8401 atattgacga atataatgat ataagaagtg gtgtagataa tttgttgaga aatgttgatg
     8461 ttaatggaaa acaaattagg ctattgggag ttacaatatc aaacctaagt gataaaaaag
     8521 aaacttataa agatattaca ttatttgaat acatggatag tatccaaatg tgagttttgt
     8581 ttaaatgaaa taacactagc tttaatacta tataattgag gctagtgtta ttttatgaat
     8641 atttttatat tttttgtttt ttgacattat gtattatata ctatatataa atactttata
     8701 tttgtagcaa tattcagaca aatagtgacc taaaaatcgt actttgttta cataaataaa
     8761 atagtatgat ataattataa aaacaactta taatattgaa gaaggttgct tgttcgaaaa
     8821 aggggatgat aaattatgat agagataaga aatgtaacaa aaaaaatagg aaataatgtt
     8881 atattagatg atatttctct agttgtagaa acaggtacat tggtagtttt gattggttca
     8941 agtggttgtg gaaagactac tactttgaag cttataaaca aattaataaa acctacatct
     9001 ggtgagatat atattaatgg caaaccaatt tctcaggaaa atgaaataga gcttagaaga
     9061 aaaataggat atgttataca gaacacaggc ctattccctc atcttacaat aaaagaaaat
     9121 atagagctaa ttccacgcct aaaaaaggaa aaatccgttg aagaaattga aaaaagaacg
     9181 ttgcaactac ttgagatggt aggacttgat agtgatgaat ttttgaataa gtatccttca
     9241 gaacttagtg gtggacagca acaaagaatc ggtgttgcaa gagccattgc tactgatgct
     9301 gaaataatat tgatggatga gccttttagt gctttagacc caataacgag aacatcttta
     9361 caagaacaat tattttctct tcaagatgaa ttgaaaaaaa ctataatatt tgtaactcat
     9421 gatatggatg aggctttaaa gatagctgat aaaatttgta ttatgaagga tggaagaata
     9481 gcacaatatg atactcccga aaatatactt agaaagcctg caaatgactt tgtaaaagat
     9541 tttatcggag aagatagagt ttgggataat ccagaatata taaaagcaag agatattatg
     9601 ataaaaaatc ctattgctgt taattctact agaactgtaa ctcaaggaat agaaattatg
     9661 agaacgagta aggttgatag cttactaatt atagatagag caaaaacatt aaaaggtatt
     9721 gttactgtaa aagatatgaa agatattgat gataagtcaa tattacttgc tgatattatg
     9781 tcaagtgagc ctttgcatgt aaatgaaggt gataatttag tagaaatact aaatgtaatg
     9841 aatcgtaatt cggttggata tattccggtt atttctgatg aaaataaact ggtagggctt
     9901 attactagaa gtagtttact ttctgtttta agtgaacaat tcttagaaat ggaggtgagt
     9961 gttcttggct aattttttta actttatact attacaaaaa gataagatta tagagctttt
    10021 aatacaacat atgagtctaa ctgttacatc tattttaata gctataatag ttggtgttcc
    10081 tcttggaata attatttcaa gaatctcatc tcttagaaag tttgtacttg gatttgtaaa
    10141 cttagtccaa gcagttccgt ctatggcact tttgggtctt cttgtaccta tacttggtat
    10201 aggaagtaaa ccagccatat ttatggtagt ggtttattca ttactaccaa ttgtaaaaaa
    10261 tacgtatata ggtataacaa gtattgaccc agtagtatta gaatcagcta aaggtattgg
    10321 tcttacaaga aatcagacat tatttaaaat acaatttcct ttagcgcttc ctataataat
    10381 gggtggagtt agaatatctg cagttacagc tgttggtttg atgactcttg cagcatttat
    10441 aggtgctggt ggtttgggat atttagtatt ttcaggggtt caaactgtaa ataataatat
    10501 gattttagca ggggcaatcc catcatgtat aattgcactg ttagttgatt atttatttag
    10561 taaaattgaa gttgcagtta ctccaaaagg attaaatcca aaagcaccta agaagaatta
    10621 tatagcactt aaagtaatat ctgtagtgct tgttgtttca atgttgttcg ttgtttttag
    10681 ttctagtttt tcaagtaaaa aagatacaat aactattggt tctaaagatt atacagaaca
    10741 acttatactt ggcaatgttt atgctgaatt agtagaaaaa aatacgaatc ttaaagttaa
    10801 gaagaattta aatctaggtg gttcatctgt tgcgtttaat gcaattaaat ctggtgagtt
    10861 agacatgtat gttgactata caggtacact tttggtaaat gtaatgaaac atgctccgat
    10921 taaagatgct gatgaagcat ataatgttgt aaaagatact atggaaaaag aaaatcagtt
    10981 aactttactt gaccctcttg gatttaataa tacttacaca ttagctatga tgcctgaaac
    11041 tgctgaaaag tatggaataa atactatttc tgatttgact aaatatggta aagaatttac
    11101 attctcacca actttggagt ttgaaaatag agaagatggt cttgtagggc tttctagaga
    11161 ttatggttta aaattcaaag atgtaaaagc aatgaatgga agtttaagat atacagcttt
    11221 agataataat gaatctcagg ttatagatgc atttttaact gatggtctta ttaaaaagtt
    11281 taatcttaag atattagagg acgataaaaa tttctttgta ccatattatg cagcaccatt
    11341 agtaagagaa gatactttga agaaatatcc tgagttagaa aaagttatga atatgctttc
    11401 aggaaaaata aatgaagaaa ctatgagaga acttaactat caagtggatg aacttggtaa
    11461 atcaccagaa gaagttgctc attcattctt agttaaagaa ggcttagtat aaaaaattaa
    11521 tagtttatat caaaaattta agggagtgtc tcaaaatgaa ctttttagtt catgaggcac
    11581 tcttttttat atgaaatcaa atatatttta tcatttcaac aataaaaaag aagattatct
    11641 ttattttgag acagtctctt atttttatgt ataccaaaac catacctacg atttttattt
    11701 ataattaagt ttcaaataga atgaacaagt acaaccaatt ttgaatataa atatagtaaa
    11761 tgtgcttata tacgatttta ttgtctggtt gattgatatt gatagatagc tttctttatt
    11821 gaatcgaagg gagggggaat ttgttatgga taactataag aaagtgaagc aagtattgtg
    11881 gataatttta tttgctaatt ttgcagtagc attattaaaa atcattattg gtaatcaaat
    11941 aaaaagctat agtatgacag cagacggatt tcattctttg tcagatggag catctaatat
    12001 agtggggctt ataggtattt tctttgcatc taaaccaaaa gataaaaatc atccttatgg
    12061 acataaaaag tttgaaatta taaccagttt atttataagt ggtatgttgt ttgttatagc
    12121 aattaagata attcttagtg cagtgttaag aatagctaat ccagttgtac ctgctataac
    12181 tatagaaagt ttaattgctt taattattac actttttata aatatttttg tatgcatgta
    12241 tgaatataga attgggacta aactaaatag ttatgttttg atttcggatt cattgcatac
    12301 aagaagtgat atatttgtgt ctctaggtgt attggttact ttagttggtg ttaagttagg
    12361 atttccagta ataatagaat ctatagttcc tattattatt tctgcattca taatttattc
    12421 ggcttatgga atatttaggc catctattgg aattttggtc gatagagtgg ctgtagatga
    12481 ggattatata aaagaaattg tgtttgaatt taatgaggta agggatgttc acaatatcag
    12541 aagtagaggt agcaaaagtt ctatttatat tgacatgcat gtaatggtag acccatttat
    12601 aagcgtagag caatctcatg atttaacaca taaaattgag aaacaaatac aagaagaaat
    12661 taatgaaaat gctcaagtaa tagtccatat tgaacctttt tatagttttt aacttagtta
    12721 taaaatacaa gttataaaaa aatataattt ataaaaaaaa tattatacca taaa
//
LOCUS       DAMJVI010000100        12626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.105, whole genome shotgun sequence.
ACCESSION   DAMJVI010000100 DAMJVI010000000
VERSION     DAMJVI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.105"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(194..1408)
                     /gene="dpaL"
                     /locus_tag="PC545_002949"
     CDS             complement(194..1408)
                     /gene="dpaL"
                     /locus_tag="PC545_002949"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2457702.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLKDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLNTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAKEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVSAKGMRILGNP
                     LRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLISTEGDTDPDKY
                     RDIVWNGECQSK"
     gene            complement(2022..2559)
                     /locus_tag="PC545_002950"
                     /pseudo
     CDS             complement(2022..2559)
                     /locus_tag="PC545_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
     gene            complement(2636..4441)
                     /locus_tag="PC545_002951"
     CDS             complement(2636..4441)
                     /locus_tag="PC545_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457703.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEASKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHGKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNIYKNKDVNIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQ"
     gene            complement(4837..5214)
                     /locus_tag="PC545_002952"
     CDS             complement(4837..5214)
                     /locus_tag="PC545_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDF2457704.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            6119..6604
                     /locus_tag="PC545_002953"
     CDS             6119..6604
                     /locus_tag="PC545_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457705.1"
                     /translation="MSKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEECSKTIKDSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEMDWKNLEKLITKENNIMQES
                     V"
     gene            complement(6804..7343)
                     /locus_tag="PC545_002954"
     CDS             complement(6804..7343)
                     /locus_tag="PC545_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2457706.1"
                     /translation="MSKNVLIVSASPRKGGNSDLLCDQFIQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYEKEANCSQTDDMGEILEKMIGADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVTAADENVADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNV
                     GDIKGSKAMKQAYEMGKLA"
     gene            complement(7336..7749)
                     /locus_tag="PC545_002955"
     CDS             complement(7336..7749)
                     /locus_tag="PC545_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2457707.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIKARKELLIEQRNQLAKRIEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEAVSYE"
     gene            complement(7785..8906)
                     /locus_tag="PC545_002956"
     CDS             complement(7785..8906)
                     /locus_tag="PC545_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDF2457708.1"
                     /translation="MEAKKLGFGLMRLPIKDENDVTTIDMEHLNKMVDTFLERGFTYF
                     DTAYVYHMGKSEIALRESLVKRHKRESFTIATKLPLMALKKKEEQETIFNEQLEKCGV
                     DYFDYYLLHNIGVSHYEVAKKFDSFKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEH
                     PEVDFVQLQINYLDWDNESIQSRKCYEVAEKHNKPVIVMEPVKGGTLAKIPEKAEKLL
                     NDYNPDMSISSWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFVQEEYNIID
                     EAVEIINESILIPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNY
                     TKTYGKASDCIECKQCEESCPQHIKIIDALKNVAETFEK"
     gene            9505..11157
                     /locus_tag="PC545_002957"
     CDS             9505..11157
                     /locus_tag="PC545_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HDF2457709.1"
                     /translation="MNKKNLAILISGMMFFSSSSAIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIENSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKSLNIKINKIVGNNIYEMSTNVLKEIDKIKKIENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSINFLNNRSIKKSYIVGGNKILSD
                     NTVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGINQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIKNKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQALPGSNILVCYYDEDDNEYDCKVIKDNILYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTQNISLDKAKSIALDAIPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIVDIDMDNDSDDDFDDNDKNNSNTNDNSSIISAEKAKSIMLDKVPGGK
                     IVKFKYDEDDKEYEGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            complement(11540..12466)
                     /locus_tag="PC545_002958"
     CDS             complement(11540..12466)
                     /locus_tag="PC545_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2457710.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSISTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSESS
                     NAEIVTKNGDIFKNIEKYIDATINNNQKTMKYKHRNELFKNSNTNIYSRINQESIDKD
                     YDLIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLTEDSTYKNPESIHEII
                     IRKLNSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEP
                     TILFGYEFESYLNEESSLSACNYGDMCYEDMY"
BASE COUNT         4253 a         2077 c         1415 g         4881 t
ORIGIN      
        1 attttaattt tatttggttt tatttaattt tatttagttt ttattttgaa tttttcattt
       61 gctttacttt ttcatttatt tagatttttc atttgtttta gtttttattt tatttaaagt
      121 tttcacttat ttaagttttt attttcctta aatttttcat ttatttgtta agtttttatt
      181 ttccttaagt cttttattta ctttggcatt caccattcca aactatatct ctatatttat
      241 cagggtcagt atctccttct gtacttatta ataatacttt agagttctca tctagtttca
      301 aagcttctct taagtctttt aaatcttcac tttcaaggat tgaaactaat gctccagttg
      361 taactgctcc agattctcca gagattactt tctcatctcc tcttagaggg tttcctaata
      421 ttctcatacc tttagcagat acccagtctg gtgctgaaac gaatgctgat gaataatttt
      481 ttaacacttc aaaacctata gtgtttggtt ctccacaagc aagccctgcc attatagttg
      541 gcatatctcc tccaacaaat cttggttttc catctccagc ttctgctgat ttatagtaac
      601 aatcagcctc atcagcctct actactactg ttattgggca ttcatcatat attgaagcaa
      661 catacccttg aactgcacca gcaagtgaac caactcctgc ttgaacaaat acgtgtgttg
      721 gtctatctac tccatattct tttaattgtt ctatagcttc tttagccata gttccatatc
      781 cttgcattat ccatgctggt atttcttcat atccgtccca tgctgtatct tgaaccatta
      841 ctccatggtc atcagcttct gcataatcat tagctaatct tactgcatca tcatagttta
      901 agtctgttat actagcatct gcaccttctc ctttgatttt atttaatcta aattcagaag
      961 aaccttttgg cattaataca acagattttt gacctaattt attagctgtc caagcaactc
     1021 ctctaccgtg atttccatct gtagctgtaa agaaagttat ttctcctaat ttttctttta
     1081 tttctttcga agtcaatttg tcatacccta actcagatat atctgtattt aatctttgag
     1141 ctaagtatct acccatagag taagaaccac ctaaaacttt aaatgcgttt aagccaaatc
     1201 tataagattc gtctttaaca tatattccgc ttacgcctag tgattttgct aagttatcta
     1261 atttaactaa aggagtcttt tcatattgtg gaaagctttc atgaaaagct ttagcctttg
     1321 caatctcttc atcttttaaa aattcaattc ctttttcttt ttcctctgat ttaggtaagt
     1381 tatttaattt ccattttatt tctctcattt attttatatt cccctttcaa atttttcggt
     1441 gtcattttaa ttttttatct ttgtgcctta tagcaaagca attaccgtgc caatattatt
     1501 tattctaaaa acaatttttt tatgttggaa ttacatactt ttataaaatt tctttaaagt
     1561 ttataaattt tgagtgtgaa aaataagatt tataaaagaa aaaattctca tattgggaaa
     1621 tttttatcaa tacgagaatt atcaattcga gaaatcgttt atttatctta attcttcctt
     1681 tattatgctc ttatttttta tatgtctatt gttttgtcag atatattact acttttattt
     1741 tctataaata tatggctata accttcttac gcataaactc tataaattta tatactttat
     1801 tacccaaata ttctttatta cccaaagcct ccaaaatggc ttaaatcttt tcatagcttc
     1861 ttacagaagt ttacatggtc gtccccccaa taagcaaaag tgcaatatgt gtaccattgt
     1921 gccctacttg cgactattga gctgtcgaaa ttcgcaactt cgcatttatc tgatgaaatg
     1981 cactatacaa aatattcata acatccacaa cagagttaaa gttacctatt catattcact
     2041 gtaaaaattt cttccacagg aaatgtaatt ttaaccaatc ctattgagac aaactcacca
     2101 cttatttttt tgaactcacg cagtaataca tcacttgccc gaaaaaaagc ctcactttca
     2161 tcatctccag taagtgctat tgtgcaatgg ggtacccaac gattggggtg gtaccattcc
     2221 caaccatttg aatcaaaatc ttgtagtacc tcatataatt ctctctgaaa ctgatacatt
     2281 gctattcatt ataggtgata caaacaccgt tttagtatca ttaaacatgc ctactgaacc
     2341 aatgtaagca ggtattacct tatggttttg tgcaaacccc tttagcttgt ctatgcaaca
     2401 agcctcatcc acatcattga aacaagccaa agtaagatga ggtcttgttt tccattctaa
     2461 gtacttcgta ctaatttttt catcagcgat ttttttagat aaatcaaaaa gttgcttttc
     2521 cgttttttta tcataatata gctctatcgc atattgcata ataacacctc caccataaag
     2581 taaaaatacc tatctataaa aacacacaaa tacctatcat tttaatatta tattactact
     2641 gttcctcttc caattttcta tatagtgtag ccaaacctat gcctaagtct tttgctatct
     2701 tcttcttcgt ctttgtatct cttccatact tattcaacaa cttgtttatg taagtcaact
     2761 ctaattcttt aattgaaagt atcctttctt gattctctac tatccctcca actatatcat
     2821 cttcaaatat tatatttaca tccttatttt tataaatatt tccattaata ttataatagt
     2881 ttaaaatatc taatggaagc atatctttat atattattcc atcatctcca accaaattca
     2941 tcatatattc aactgcattt tccagttccc ttatatttcc cggccaatta tagttaatca
     3001 acatattcat tacattttct tctattttat ttaactgaat acctaattct aaagaatatt
     3061 tagaagcaaa cttcatcatt attgcctcaa tatcatcctt tctttcccta agaggtggca
     3121 aatttatagg aattacattc aatctatagt ataaatcatc cctaaactta ccttcattta
     3181 ctaatttcaa taagttttta tttgtagcag caatcactct tatatctaaa tctataagtt
     3241 tatttgaccc aattctcgca aattttctct cttgtaaaac tctaagtaac tttgcttgta
     3301 aaggcattga caaatctcct atttcatcaa gaaatataac accttcattt gcaagttcaa
     3361 atttacctac tttccctcca ctactagctc cactaaatgc accttttata tagccaaata
     3421 gctcactttc aaggagattt tctggaatag cagcacagtt tatcgcaata aatggcttgt
     3481 tccatctgct accttcagca tgtatagccc tagcaattag ctctttaccc gtaccacttt
     3541 ccccagttat aagtacagtt gaatttgact ttgctaattt cttagttctc tcttttactt
     3601 ttaacattgc ttctgaattt ccaattatag actctatatc actatttccc cagccactat
     3661 tcacttttcc atgtccttta tgattgatat atgtcttgtc aaatattatt attttgtcgt
     3721 attgatttat atatggatat accggaatta ttttggcatt tatattataa attttgttat
     3781 caatatcaat ttctagtaat tcatgtccat ttgaggaaga ttcaacagat actatattta
     3841 ctatattttc tattatattt ttatctatat ctaatttttt tagagctata ttgtttatat
     3901 aagatatttt gctatttatg tctagtatta taacgccttt ttcaacattt tcaatgattt
     3961 ctatcatcat atccattttt ctactgctat tttttgacgc ttcatgttct tctattttca
     4021 tagaaattaa atcgcatata tgattgacaa attttaaata attatctatt tttgcactta
     4081 tttctacttt tttagtttta tctgtgctaa tagcaccaat tactcctata gttcgattat
     4141 tatactttat tggaactgct attattactt tatttaggca tttcatgtaa tgggaacatt
     4201 ctatacaaag ctgattatca catatatcca atacgactaa ttcctgacct gtctgtataa
     4261 cattgtcata aataaagccc tcagtaacaa cgctttcatt tatctttgat ttatacagac
     4321 ctgtaccact aattcttatt aatctatcgt caactatttc aatatctaca tctaacacac
     4381 tagctactgt ttcagtgtat tttatcattt cttgttgaaa ttcttttaat ttacttaaca
     4441 tagtttcccc cattagaatt taatttacca aattatcagt ttagctatat tttaacatat
     4501 agtcactctt taaaatatat ataaaacatt acttatttat atatttgcgt taaaacagac
     4561 aatattaatt tataaaaagt ttgttttata tagtaacctt aattataata tgcagacaat
     4621 aaaataaatc acaatacaat aataaaaaaa agctttcctc atataacaaa tatatttttg
     4681 acataaaaat atacattata tgagaaaagc tcaatagtta aatatttaga tagattttta
     4741 tatagacttt tttaatataa acttttttaa ttgtagactt tttttattag tttttaattt
     4801 ttattagttt tgttattggt tttattgtta taatacttat aatactgcca ttgcttcaat
     4861 ttctaaaact gcatcttttg gtaattgagc aacttgtaca catgaccttg ctggtggatt
     4921 ttctttaaaa tatgtagcat atacttcatt aatatcatta aaatccttta aatcttttac
     4981 gaatactgta gtcttaaata ttttatccat acttgtacca gcttgttcta atattgcttt
     5041 tacattatct aaagaagctt ttgttgcttc ttttgggtca gatactaact cgccagtctt
     5101 catatctatt ggaagttgac ctgatgtaga aactatgttt ccaacttgaa ttgcttgaga
     5161 atatggtcca attgctcctg gagctttctc tgtagctatt actacttttg acatgacttc
     5221 acctcctatt attaagttat atttacagga aaataaaatt tcctaaacac caatattaat
     5281 acgaaccttt agattaaact tcaaatctaa aacttcaaat aatcaatact tagatgatta
     5341 tgtttatttg tgtcttgtta acttttatta aagcaattaa cgtgccaaac tttatttata
     5401 tataaaattt tgtgaagtca ttcaaaatag ttacttctga attttttcgt aaaaaaacgc
     5461 tctaaaatta cttttttatg atgtcaattt ttttctcata tctagaaatt taccaacatg
     5521 agaattcttg ttttgagaat ttacagatta aagttatttt ttaaccttaa attctgaacc
     5581 tcccatgact aaagtcacag ggttcctgct tcatagaatt ttgcataatc aagttattac
     5641 atatgaaaga actatagttg acaatcccac tattataaat aataaaacct ttacccatac
     5701 cttatttacc ctagttatca tagagcctat tgaaaatata acagctaaat ataataataa
     5761 ccaaaaaatt atcaatatct accactgttg ctgaaaatcc aaaaaactac cttttgatag
     5821 tattacttaa caatctaatt aatctcataa taaatttaca acttataaat aaattgttat
     5881 attggccgta tctcaacaac tttctccttt cctagtttta aatcttttat agaattgtac
     5941 cacgtctaag taaattttca tactattaag tcttatttct aataaatcat tagctctcat
     6001 tctatgccca ataatgtaag attgcagtaa ttttattgat tttaatagtt ttatttaata
     6061 tacttatatt aattattttc atatgaaatt attatttata tagggggatt tttttaatat
     6121 gagcaaaagt gaattaacag cagaaacaac agaagaaatg ttagaagtac taagtggtaa
     6181 agattatgat attgcatgtc atttacatga acttggtaaa tcattagatt gtaaaattga
     6241 accaaaaaca ggtgctcgtt cttacaaaat agtatattca actaagaaac caaaacgtag
     6301 cttatttact attgaatgta atgaaaaaaa atggagagtt aaagcaaatc tttttcatct
     6361 aaatacatat aaagatgctg tggaggaatg ctctaaaact attaaagata gtataactaa
     6421 aactcgtact tgtaagaaat gtaattcaaa gtgtattgga ggttcttcgt ttgaattaga
     6481 tggaaagtct tacctgactt gcataggaag tggtcattat tttgcaaata tggaagaaat
     6541 ggattggaaa aacctagaaa aattaattac taaagaaaat aatattatgc aggaatctgt
     6601 atagtttttt aagggtaatt attaaaatat atgagtaaca acagatactt aaataggcat
     6661 aaaaaaataa agggtataat aaggatatag aataaaaata aatgatatag cacaaagagg
     6721 ctgtctcaaa atacagataa gcctcttttt attgttgaaa tgataagaat atattcaatt
     6781 ttatacaatt ttatacaaac atactacgcc aacttaccca tttcatatgc ctgtttcatt
     6841 gccttacttc ccttaatatc acctacattc caagcaccta caccatatac tatacctttt
     6901 tccttagaac catttagaca tgatgtaaat cctctaaatc cttctattgt tctttccata
     6961 tcagccacat tttcatctgc tgctgtaacg ataaaataga aatctttatc acaaatttca
     7021 gtatatctag agcaagttct atcaatcata gttttcattt gcgcattcat agtataaaaa
     7081 taaactggag tcgctaaaac aatcacatct gccccaatca ttttttcaag aatttctccc
     7141 atgtcatctg tctgtgaaca atttgcttct ttttcataac aagcaccaca tccagtacaa
     7201 tagttaatat ctttatcttt taaaaatatc ttctccactt gatttttaga ttctaatacc
     7261 ccttgtataa actggtcaca caataaatca gaatttccac cttttctagg actagctgat
     7321 actattaata catttttact cataagatac tgcctccatt ttcttatttc tactgctctt
     7381 taatactttt tctttctcaa taataccttc ttcatatcta tcaatcttaa aatttaatcg
     7441 ttccaaagtt ttttgcattt cttctattct ttttgctagt tggtttctct gctctattaa
     7501 aagttctttt ctagctttga tagtactgct cccttgttga aacatagcta catactcaat
     7561 taatgtctca atagaaagac ctgcacctct catacacttt atgaaattaa cccactcaca
     7621 atcttcttct gtaaaagacc taattccact tttatttcta tttacaggtg gtattaaacc
     7681 aattcgttca taatatcgta aagtatccgc tgaaagctcg tattttttac taacttctga
     7741 aatcgtcata catatcttct cctcttgata ttgtatagat tgaattattt ctcaaaggtt
     7801 tctgctacat tctttaaagc atctataatt ttaatgtgtt gaggacagct ttcttcacat
     7861 tgtttacatt caatacaatc tgaggccttt ccataagttt tagtataatt attatagtag
     7921 accatctgtg ttgaaaatcc agtattaaga gcttgttttt ctgcattgta caacgcaaaa
     7981 tagtttggaa tagcgatatt ttttggacag ccttctacac aatactgaca ggcagtacaa
     8041 ggaattaaaa ttgattcgtt gataatctct actgcttcat ctataatatt atattcctct
     8101 tgtacgaatg gtttgaaatt ttgcatatat cctgtattgt ctaataattg ttccatattt
     8161 gacatcccac ttaagaccat cataacatta tctttacttg ctgcaaaacg tattgcccaa
     8221 gatgaaattg acatatctgg attgtagtca ttcaataatt tctctgcttt ttctggtatc
     8281 tttgcaagtg tacctccttt tactggctcc ataacaatta caggcttatt atgcttctct
     8341 gccacctcgt agcattttct agattgtatg ctttcattat cccaatctaa ataattaatc
     8401 tgtaattgca cgaagtctac ttctggatgc tcactcaaga ctttatccaa taattctgca
     8461 ctatcatgaa aagaaaaacc aatattcttt atttttcctt cttttttctt ttcttcaata
     8521 aacttaaagc tatcaaattt tttagctact tcataatgtg acacgcctat attgtgtaaa
     8581 agatagtagt caaaatagtc tacaccacat ttttctaatt gctcattaaa aatggtttct
     8641 tgctcttctt ttttctttaa agccattaat ggaagttttg tagctatagt aaaactctct
     8701 cttttgtgtc tttttactag agattctcta agtgctatct cactttttcc catgtgatac
     8761 acataggccg tatcaaaata tgtaaatccc ctttctaaaa aagtgtctac cattttattt
     8821 aaatgttcca tatcaatagt tgtaacatca ttttcatctt ttattggtaa acgcattaat
     8881 ccaaatccta atttttttgc ctccattgtt ttacttcccc ctttaaaact tgtttgtttt
     8941 ttctctctac tttaactatt cttacaagta tataattctt gtataattat actttactac
     9001 ttggagttaa cttcaagtca atagattttt ttatttttaa gacaaatatt ataaaaatac
     9061 agttctttaa tattaatcca ttacatgttt ctttcaccct ccaattctca aacttcacaa
     9121 actccaacat gtaaagaaat atatacttta agaaattcag tccattaata ttactacaaa
     9181 tactttttag atatgtttaa aaagaaattt cacctaaaat atttataaaa gatttagaaa
     9241 taggtcttgt gtctatttta aaatatataa aacagtgata aagacttatt tataagataa
     9301 aaaatgaata gtgtataccc tttttaaaaa gttttttcct ttttatattt gtgatacaat
     9361 gttaaatata aaaaggaaaa ttttgtatta aagaaatctt atttgttgat tttgtaactt
     9421 caaactcagt atcaatttga ttttaagatt atattttatt aagtatgcta aataacaaat
     9481 ttttaaagat tgtgaggtaa aaatatgaat aagaaaaatt tagctatatt aatatcaggt
     9541 atgatgtttt tttctagttc ttcagcaatt tttgcagaca atactacaaa gcaagaaaga
     9601 ttaataggta aaacaagata tgaaacagca gtagaagtaa gtaaattggg atgggttcaa
     9661 tcaaaaacag ccattatagt taatggtaat tctattcaaa gtgcgctttg tgcaaatcca
     9721 tttgctaagc taaagaatgc acctatactt ttagtaaaca acaacagtat tgaaaattca
     9781 actaaggctg agcttaaaag gcttggtgta gataatgtgt acattgtaga tagtggaaat
     9841 agcatttctt ctaaagtaga aaatgaaata aaatcactta acataaaaat aaataaaata
     9901 gtaggaaaca atatatatga aatgtctaca aatgtactta aagaaataga taagataaaa
     9961 aagatagaaa atgttgcagt tgtaaatgga ctaaagggac tatctgatgc agttagtata
    10021 gcttctccag cagcaataca taatatgcct ataatattag tatcagatga aaacagcaat
    10081 gatagtagta taaatttctt aaacaatagg tctataaaaa aatcttacat agttggagga
    10141 aataagattt tatcagacaa tacagtaaag aaatatccaa acagagaaag aatcagtggt
    10201 tcagatagaa acaatactaa tgcaaaaatt attgataaat tttataaaga taaaaaaata
    10261 aataatgtat ttgtagctaa agatggtatt aaccaagaaa atgcattagt agattcatta
    10321 tcaataggag tactagctgc taaagaaaat tctcctataa tgatatctaa tggtagcctt
    10381 gtaaatagtc aaaaagaatt tattaaaaat aaagatatag atgaaataac acaagtagga
    10441 ggaggatata atcaaaaagc atttgaagat attttaaaac taaaaaatgt gaacataaga
    10501 aataaaacta aaaaaatatc tttaatacag gctaaagaaa tattaaaaca agctcttcca
    10561 ggaagcaata ttttagtttg ttattatgat gaagatgata atgaatatga ctgcaaagtt
    10621 ataaaggata atatcctata tgagatagaa attgatgcct actctggtaa aataatagaa
    10681 attgaaaaag atgatgactt aaatgataat aatttaggta cacaaaatat atcattggat
    10741 aaagctaagt ctatagcatt agatgctatt cctaatggta atttaatcta ttgtaaatat
    10801 gatgctgaag ataacgaata tgaggctaaa gtaataaaaa ataagaaaac atatgaatta
    10861 gagataagtg catttgatgg aagaattgta gatattgaca tggacaatga ttctgatgat
    10921 gactttgatg ataatgataa gaataactct aatactaatg acaacagttc tattattagt
    10981 gctgaaaaag ctaaatcaat aatgctagat aaagtgcctg gtgggaaaat agtaaaattt
    11041 aagtatgatg aagatgacaa agaatatgaa ggtgaaataa taaaaggaaa aactgaatat
    11101 gagataacaa ttaatgcaat tactggtaaa atattagaat ttgaaataga tgattaataa
    11161 aaaatataaa tagatgatta ataaaaaata tatattctat taattattac aagcaataaa
    11221 gccataaata ttgaaatttt caatatttat ggctttattt tattttttta atttaaatcc
    11281 acattatgcc attatatata tctatgcact cttgtaaaat gggttatatt tctccagtat
    11341 attaaaaaaa gtgagtgtct caaaataaat cttctaactc ataaagcact ctcctttgta
    11401 ttaaatcaaa tatattttca tcatttaaac aattaagaag agacttctct ctattttgag
    11461 acaactccat tttaatacac tatttataca taaacatatt ttaaacaact aaaaagcaac
    11521 taatatctta ataaatatct caatacatat cttcataaca catatcacca taattacaag
    11581 cagataaaga actctcctca tttaaatacg actcaaattc atacccaaac agaattgttg
    11641 gttctatata tatttccata tctgtatatc tccatcttct tacctttgcc tctataacct
    11701 tgtaaaaatc ttctaaacta tacccttctt ttaatctttt atctataagt gccttagtta
    11761 tactcaaatc agctttaaaa ttttttcctg attcactatt taattttctt ataataattt
    11821 catgtatgct ttctggattt ttgtaagtag aatcttcagt caaatcttca tccgaatttt
    11881 caaaggtatt tttatttaaa ttctttttat ttaaattatt ttttaataat tcttttttct
    11941 ttgtctcatt ttctgtttca tacccagtat gaattatcaa atcataatct ttgtctatac
    12001 tctcttgatt tatacggctg tatatgttgg tattactgtt cttaaacaac tcatttctat
    12061 gtttatattt cattgttttt tggttattgt taatagttgc atcaatgtat ttctctatat
    12121 ttttgaaaat atcaccattc tttgttacaa tttctgcatt actactttca gaacaaacat
    12181 aactatatac agaacaacaa tttcccttta ctcccctact tattaatctc aatataccta
    12241 aatctataaa ttcatttaca agtctactta ttgtagatat agatacatca aggtcagatg
    12301 cagctttcct tactgtcatc ataaattgtc ctcttggcag agttcctttt aatctttgta
    12361 tattttcttt tctcattaca tagtgattaa accttattct atttacatct cttttttcat
    12421 taaaatataa tactgctgaa tttaattgat aaaaactatt actcatattc ccactcctcc
    12481 aatttaaata tataatttgt tgtaaagttt ttagattttt aaataactta ttaaatgatt
    12541 atttttatta aaaagtattc tttgatattt tcattttaaa gttttaagta taataagtta
    12601 atagtgtaga tttaactttt ttattt
//
LOCUS       DAMJVI010000101        12435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.106, whole genome shotgun sequence.
ACCESSION   DAMJVI010000101 DAMJVI010000000
VERSION     DAMJVI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.106"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            328..1263
                     /locus_tag="PC545_002959"
     CDS             328..1263
                     /locus_tag="PC545_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDF2457711.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSNNPNGFWIILLITSIICIVITFFMSRNKLL"
     gene            complement(1355..3226)
                     /locus_tag="PC545_002960"
     CDS             complement(1355..3226)
                     /locus_tag="PC545_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDF2457712.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKMESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFVRCEKWLKTAADKNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNIEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     SKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEGFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDS"
     gene            3416..4342
                     /locus_tag="PC545_002961"
     CDS             3416..4342
                     /locus_tag="PC545_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2457713.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTSRGFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLVLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4481..4627
                     /locus_tag="PC545_002962"
     CDS             4481..4627
                     /locus_tag="PC545_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457714.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4732..5256)
                     /locus_tag="PC545_002963"
     CDS             complement(4732..5256)
                     /locus_tag="PC545_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457715.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     EAMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLTRVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5309..5533)
                     /locus_tag="PC545_002964"
     CDS             complement(5309..5533)
                     /locus_tag="PC545_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457716.1"
                     /translation="MIIKLTKDNFNFRFKQYEGMFFHAEVVEGMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQVEAYIDLNSIK"
     gene            5730..6854
                     /locus_tag="PC545_002965"
     CDS             5730..6854
                     /locus_tag="PC545_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HDF2457717.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     ILSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIYLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLKNNTSIQSKQYSVKELTKLYKFLVIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPGVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESEFWQKYEGKIN
                     EISNEFNNSYLKANSVTEGTQSYGKMVDLLLTYYELYPYN"
     gene            complement(7328..8152)
                     /locus_tag="PC545_002966"
     CDS             complement(7328..8152)
                     /locus_tag="PC545_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457718.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTNVYLANTVS
                     TVTKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
     gene            complement(8500..9555)
                     /gene="ispG"
                     /locus_tag="PC545_002967"
                     /gene_synonym="gcpE"
     CDS             complement(8500..9555)
                     /gene="ispG"
                     /locus_tag="PC545_002967"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDF2457719.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            complement(9737..10741)
                     /gene="rseP"
                     /locus_tag="PC545_002968"
     CDS             complement(9737..10741)
                     /gene="rseP"
                     /locus_tag="PC545_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDF2457720.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFLGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            complement(10754..11908)
                     /locus_tag="PC545_002969"
     CDS             complement(10754..11908)
                     /locus_tag="PC545_002969"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDF2457721.1"
                     /translation="MKKISILGSTGSIGKQTLDVVRDNRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELANITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYTDSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            complement(12072..12275)
                     /locus_tag="PC545_002970"
     CDS             complement(12072..12275)
                     /locus_tag="PC545_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457722.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKSI"
BASE COUNT         4147 a         2090 c         1295 g         4903 t
ORIGIN      
        1 ttaaatataa cttatatact ttatgtgtaa attatggtaa aaatattatt taatcaaatc
       61 aaaacttgat ttatatacct ggaaaaggta ttatattaaa gagtgcttaa aatacgcatt
      121 tcataaatag ctatatataa aataataaat aaggagagtg atgggcaatg gatactagtc
      181 cgttgcggag agaatttttt acattttcat cacttaataa tatctaaaat caaaactaat
      241 acatatattc agttatataa attagttaat taatagatac tattaaatga tgagatttat
      301 ataaaactga ataaaggatg tgttgccatg ataagatatt acaaaactat agattcaaaa
      361 ttagagaaac ttagtttttt tgaagatggc tgttggataa atcttgttga acctaatcat
      421 agtgaaatca acgaaatttc taatttatta aatatagatg tagaaagcat agaatctgca
      481 ctagatgaag aagaacgctc aagaatagat gttgaagaca atcatactct tatacttata
      541 gatataccag tagacgaaag tgactctaat tcttctcatt atactacaat acccttaggt
      601 attatactaa ctgaagaagc tattgtaaca gtttgtgatg cacaaactaa gattttaaat
      661 gactttatag ttggtcatat taaagatttt tttacgttta aaaagactcg ctttttgctt
      721 caaattcttc acaaaaatgc agcttattac ttgcattact taagaaaaat aaataaaatg
      781 acaatcataa tagagcgaga aatttacaaa tcaatgaaaa acaaagaatt ggttcaatta
      841 ttggaattag aaaaatcttt ggtatacttc tctacctcac taaaatctaa tgaacttgtt
      901 ttaaacaaaa tggttagaac tgctggtata aagaagtatc cagatgatga agacctatta
      961 gaagatgtta tagttgaaaa tagacaggct cttgatatgg ctaaaatata tggagatata
     1021 ttgagtagaa ttatggatgc tttcagtgca attataagta ataatcagaa taatgttatg
     1081 caaattttaa cagtagttac actaattttc tcaataccta caataatatc aggattcttt
     1141 ggtatgaatg tcatcaacat gccattttct aataacccaa acggattttg gataatactt
     1201 cttattacct ctataatatg tatagtgatt acatttttca tgtcaagaaa taagctatta
     1261 taaaatatta ataaaaaagc cattagaatt aattgattta catttatcct aatggctttt
     1321 atatatttaa atctatataa tcttataata ctttttaact atcatgttct tcatctataa
     1381 tcatttcagt ttcaagccct tctagcattg aatctaaact ttcttttata tttagtatat
     1441 aaattggtac atactcttct tcatctatta cctcttcttg caacttaaat ggaggaatta
     1501 atacttcatc aaagtctata tttaaatttg gaaagccttc tactatctct tcacaattat
     1561 cgtactcttc atttgctaaa gaaatgtaca tatctaggct ctttttataa taaagacctg
     1621 caagcatata ctcacacttc ttacttccat atttaatacc ttctttataa tatttctcag
     1681 cttctgcgtc atcatttaat tttaaataaa tttgacctaa attatatatt gattcaacac
     1741 aaccttgtgc tctagccttt tcataccaaa atttagaatt tacataatct ttatagaaca
     1801 tatcataaat taatcctacc atatgttgag catacacatt attttcattg gcaggtgtat
     1861 caaacatcat tttagactct tccaatttac cttctctaaa ataaattcta cctagatgta
     1921 tacgagcatt catatggttt ttgctacttg ccatttcaaa atactttcta gcattttttg
     1981 tatttcctaa gctctcatat atacacccca atctataact agacctcgta tctccatttt
     2041 cagatgcttt cttataccaa cttattgctt cttcaatatt atttaaatct tcatagatat
     2101 ttcctaattt gatttgacaa gatacattat ttggaatcct cgaataatac gaaatcgctt
     2161 tttctatatc ttgattctga taatataaat cccctagatt ttcaagggaa cttttgcatc
     2221 caacagctat agcatcctca taatatttaa tagcattttc ataatctttt tcttcaaaat
     2281 atatacctgc cagattattt tttgcttctg ttatatcatc ttcagcagcc attttataat
     2341 aagtttttgc cttctctatc tcattttctc tattatataa attagcaagt ctatacttag
     2401 aaagtgtatg atttttttct attgctttct cataataata aattgcatct cccagctctt
     2461 cttttctatc atatacatat gcaagcctat actcagactt cacatcatct ctatcagcac
     2521 tctttttata atacttctct gcatcttcta aattatcatt tatttccata atcataccca
     2581 agttaaagca acctttcata tgacctgact tgtacaactg tttatataaa cttactgttg
     2641 atttattatc tttctttttt ccatttaata ttgctaaatt atattttgca tcatcatcta
     2701 aaagcacaga acctttttta tagtattcat atgcttcatc atctttctcc aaaaaagtat
     2761 ataatacccc taaattaaaa caagctttac ctaaattctt gtctgctgct gtttttaacc
     2821 atttttcaca ccttacaaaa tctttcattt taaaatggca tacacctaaa ttattttgtg
     2881 cttgtaaata acctaaattt gctatctttt cataccaata tatagcatat tcatagccat
     2941 ctaacttata gtacatatta cctaaatcaa aagcagcatc ttcacttcca cttttatatg
     3001 ccattgaata ccacttttct gcttctttaa attgtttttt actttcatat atatttccta
     3061 aagtatattg agattttacg taattatcca atgcagcact cttatacaac tctattgcgc
     3121 tttccatctt acccatcttt tcaaggaggg cacccttagt atgtacgact ttcgggtcat
     3181 tagatatatt ttttattttt tttatagaaa attttgtatt tttcattatt cgctacctac
     3241 ccattttata aaatatattc tctcaaacat tattatacat tataatctac aattaatgct
     3301 ataatattga atttttacct tttgtcacta tattgtaaca ttaaatttta aattttataa
     3361 tcattagatt ttattatgta attggtatat aatgtatata taggggggac attttatgaa
     3421 tttcagcaat atttttatcc aagttgcagt tttatttata attattttag ttggatattt
     3481 tgtcagaaaa ttcaacttat tagatgacca ctgcacatct aagctttcaa ctttgacaat
     3541 gacagttttt ttaccatcaa tgataattag ctccatgcaa attaactttg atagtaaaat
     3601 gatacaaaaa atactattgt tattatttac atctttgatt atgtatatag tttcaattat
     3661 aattgcattt ttattgaaat acattttaaa atgtaatgat aaaaaagatt tgggaatata
     3721 ccagtatata gttgtttttt caaatgttgc ttttatgggt tatcctgtaa ttgaagcagt
     3781 tttaggtcat gaagcaatat tctatactgc aatattcaat ttgcctttta acttattttc
     3841 tttcacttta ggaatttatc ttttatctaa aggcagtaca agtagaggtt tttcaataaa
     3901 atcacttata agccctgcta caatagctat tgttattgga ctttttttat ttgtaacagg
     3961 acttaggtta ccccaattta taaacgagcc acttgagatg ttgggaagta taactacacc
     4021 aatatccatg attatcattg gaagcttact tgctaattct tcagctctcg attgttttgt
     4081 caacaaaaaa ttatatatag tcacttttat aagattattg gtacttcctg tgattgtgta
     4141 ttttattcta aaaggatgga taaatgataa gatgatttta gctataccag tagtaatttc
     4201 ttcaatgcct gctgctgcaa atactgctat aatggctaat caatatgact ccaatataac
     4261 actagcttca caatgtgtgt tttttacaac actattttca gttatatcta taccttttat
     4321 aagtatttta ctcttatctt gatttatttt tttatgtatt atataataac atattcaata
     4381 aattacaaat ataaacattt tattgtagta tattgatgga attttttcct agatatttgt
     4441 caataatatc tatatgtatt atagaatagg aggcttaaat atggatatgg atttactctt
     4501 taaaatagta tttgctatag ttgctatagg tgtttctgta aaaatcatta aaactgttgc
     4561 tagtttagct tttaaaatag cgttgatatt gctaataatt ttatttgtat atagaatgtt
     4621 tttgtaaaca tttaaaaata agttttttaa cacaatagag aagtaaaata gattatcttt
     4681 aatgtaaatt ttttattaaa gacactctat tttacttctc tattagctta tttaaattaa
     4741 atttttatta tatcttccca taatataaac atcttcaaat tttccatctc tgattacagc
     4801 atatttttta gttccctcta tttcaaaacc tagcttttta tataaattta atcccttttc
     4861 attatccaca attacttcta atcctactct agttaacata atccaattat ctgctatatc
     4921 taaaagttgt tccataagct tcttacctac tcctcttccc tgataattct ctgcaacact
     4981 tattcctatt tctgcgctat gtctcaatcg tggagaagaa tttacatgta acccagcaaa
     5041 acctattaca tctttttctc cattattatt agtttcagct acaaacatat aatcattttc
     5101 tcccaaagaa gctaacatag cttcagtttt atcgactctg tcactcatta atgccaaagt
     5161 atttctcata acactgtatg ttcttctaat ttcattaaaa gcttgtgcat cttctcttct
     5221 tataggtctt attgttatat ctataggctt actcatattg gattccccct caaacataat
     5281 ctttattttt aattaaaatt ttacatcttt attttatact gttcaaatct atgtatgctt
     5341 ctacctgagg tacatatatc atattatctt ttatatgtgt acttccaaat tcttttaatt
     5401 ctgtaccttc tttatcctta gttattttat atctaatgga ttccatacct tcaactactt
     5461 cagcatgaaa aaacatacct tcatattgtt taaatctaaa attaaaattg tctttagtaa
     5521 gttttattat catattttta tctactcctt atataattga tatttttctt attattagtt
     5581 gtatatatac tgcttattca tattaatata ttataactta tcatttattt agtataaagt
     5641 tataaataga tatatttata cttattttac aatctttctt caattgatat ataatatgtt
     5701 tatataaact aatgtatgtg aggtgaaata tgagcaaaaa aacaaaatat ttttctttaa
     5761 ttctctttcc tattgctttg cttttaaatt ttatagccag caaagttcca aatattgttg
     5821 aaaaatatta ctcccaattc atagataaaa taattgtaca aatattgagt aaagtttctg
     5881 gaattttccc attttctctc tatgagatta caatgtacat aatagttata tctatcttct
     5941 tatttttatg ttgtacttta tctacaattt ttaataagaa aaaaaaatta aacatatatt
     6001 taaaaaattc cattttaaat atattatcca ttgtatcaat agtctacttt ctttttgtag
     6061 tgctttgggg aataaactat aataggattc cactagaaac cacacttata aataactata
     6121 atttaaaaaa caatacctct atccaatcaa aacaatatag tgttaaagag cttactaaat
     6181 tatataaatt ccttgtaatt aaagcaaatg aaactagaaa actcacatta caagataaaa
     6241 atggtattgt aaaatctaat acagattata atggtgtcat aaatagagct caactaggat
     6301 acgataatat cttggatata ttacctggtg ttagtggtag ttattctaag cctaagtata
     6361 ttatttcatc aaatctaatg tgctatacag gaataactgg tatatacttt ccttttacag
     6421 gtgaggcaaa tgtaaatata gcaatacctg acttatatat tccttgtaca gttgaacatg
     6481 aaatggcaca tcaaagggga tttgctagtg aagatgaggc taattttata gcgtatctta
     6541 cttctataaa acaccccaat attgatttta actattctgg atatatttta gctttaaatt
     6601 atactgcttc tgctttaagt aaggttgatt ataatgcata tgttgatatt tctgctggca
     6661 tttcagattc tgtccgtaga gatttaaaaa atgaaagtga attttggcaa aaatatgaag
     6721 gtaaaattaa tgaaatttct aatgagttta acaattctta cttaaaagct aatagcgtta
     6781 ctgaaggtac tcagagttat ggtaaaatgg ttgatttatt gctaacttat tatgaattat
     6841 atccatataa ctaaacacta caattcaaaa ttttattcat atacagctga tattaaatca
     6901 gctgttttct atttaaataa attatttaac ttcaagcaat cttatataat ttgatagtac
     6961 ttatactatc tgtataacat ttcaattcta actattttag ttaaaacatt tctattatct
     7021 tttttaataa atgttttaac taaaattttt aaaacataaa acttccacat tatttcacta
     7081 tctatataaa aatctaaaaa ttcacatata aaattacata actaaaaaat cataaattta
     7141 tagaaaacat atattcaaaa taaaaaagca tgtatcaaaa cttgacacat gcttataatc
     7201 ttatgtatat atatcatttt gtcacaataa aaatttactt gtaacactga tatttttaaa
     7261 caaaatagtc cttatttaat taaattaaca ttaattaaat tcgtactcat tttgctatca
     7321 ttaaacatta cttatagtaa ttttctaata cactatttga aacttccttc ccttgaaaac
     7381 ttttcaacca attctcatat gctgagtttt tccatacact accatcatta aatgttactt
     7441 gttttacaca agatataatg tactttacac catgctctcc agttgattgt ttccatttat
     7501 caaacaatag aaaggcatca ttttgattct tacctggtgc tattgcattt tgctttgcat
     7561 attctttctc catttgtttc cattctttca accaattttc tacccatgcc ttgccttgtt
     7621 gtttttcaaa catacttatt atatcctgtg gtggtgattg ttgcattttt ctataagtat
     7681 gtgaatatga ttttggagac acaggagata tgcctgtaac aataccatag tctacaccag
     7741 ctgggctaaa tccaacactt cctacttcgc catctgctgc cacattttga gcatcccaat
     7801 atagctcaag tggttttcca tttttatcat atgctaagca tgtaatttca taatccataa
     7861 ttgccttttc actgctattt gttaagctta taacattgct aggccaatga tatggagtat
     7921 catcatactg tattttgcta ttatttataa ataatggaat attctcatta taaacaagac
     7981 ctttactact ttgaatatct gataccttct ttgtgacagt acttaccgta tttgctaagt
     8041 atacatttgt tttttctgaa ttggtcgtac taagagaaaa tgttgttgtc gttccagtaa
     8101 tcagcaaagc aacaactaaa ataggtaagc tagatatttt tttatatctc acaattcttc
     8161 acctcttttt ctattaactt ataataaaac aatttaccaa aacattaaaa tctatgttaa
     8221 cataatttac caactgttct agtaactata tcctatcata gttactatca agtttcaata
     8281 ctattgatgg aaattttcac tatattaaat ctttttttaa ttttataaat ttacaatatg
     8341 atttaactca tacatatata gtcaaattag aatataagtt tcttacattt aatgaagttt
     8401 ataaaataaa aaatgactag ataattaaat actgtaaata aaaacttatt acaaaagtat
     8461 ttaaaaatct agtcaattaa tatatatatg aattgtatac tataattttt caatctcttc
     8521 tagcaactca tcaactaatt tgttaccatc aatcattcta actatttcac cctttttaaa
     8581 taaaagacct tcacctttac ccccagcaat acctatatct gcctctctgg cttcaccagg
     8641 tccatttaca gcacagccca taattgcaac agtgatattt ttttccatac taccaatctt
     8701 ttcttcaacc tcatttacaa cactaattaa gtctatatta catctaccac atgtagggca
     8761 tgatataact ttaatcttat catttaaaag tcccaaactt cttaaaatct gtttaccaac
     8821 aataacctct tcaacagggt cgcctgttaa agatattcta acagtatctc caattccttt
     8881 aagaagaagt gctcctaccc ctatagaaga ctttattgtt cctttgtgaa cacttccaga
     8941 ttctgtaatt ccaatatgaa gaggataatc aacttttttt gatattaatt catatgcatc
     9001 aacagtttta taaatatcgg atgattttag agatataact atattatgaa aatctaagtc
     9061 ttcaagaatc ttaatatgtc ccattgcact ttcaactaat gcttcagcag ttgcagagcc
     9121 atactttttt aaaagttctt tctctaaaga acctccatta actccaattc taatttttaa
     9181 atttctttcc ttgcatttct caactaccat cttaactctc tctatacttc caatattacc
     9241 aggatttatt cttattccat ccacaccttg ttctatagct tcaagggcta gcttataatc
     9301 gaaatgtata tctgcaataa ttggtatatt tacactagac tttatctttg ctatattttt
     9361 agcagcttta atatcaggaa cagcaactct aactatatca caaccagctt cctctaatct
     9421 ctttatttga gctattgtag catctgcatc tcttgtatca gtatttgtca tagattgtat
     9481 acttatagga ttttctcctc ctatttttac agttcctaca cttacttctc tcgtctttct
     9541 tctctcgtaa ctcatattcc acctctatat attattttat attgaatctc tttaaatatt
     9601 aaaatattat aacctacatg tatattataa attaaaaagc tatctagtta aataagaata
     9661 aatttcatat ataaaaatta atttattctt atttaaaact acatagccta ttttcttaaa
     9721 atctctaaaa actaaatcat tgaaataatc ttagtatatc tttatatgtg ataaaaagca
     9781 taaataacat taaagctcca aaaccaacaa catttatcat gccttcttta tttgggtcga
     9841 gttttttacc acctctaaca gcttctagta acagcataag tattctccat ccatcaagtg
     9901 ctggtattgg taatagattt actataccta aatttaagct tatgacagct gctaaataca
     9961 ctacatttat gatgcctgta cgagcagcat ctgataccat accaataaca cctactggtc
    10021 ctgctacagc attgccaatc cctcctggta cacgtcctgt aatcatttga cctagaaatg
    10081 ttagcatctg ttttgtcata tctacagtag ttttacatgc atttactatt gaccccaaaa
    10141 agtctttttc tctttgtggt tgaatcccta tttcatactt tccattgtta tttttaggtg
    10201 ttacatttac aactttatca cttccatctc tagttatact aagttttaat tctccaccag
    10261 aagaagtatt tattatattt gcaacttcat cccaagtctt aacagaatta ccattaattt
    10321 tatttatctt atctcctact tgaagcccta cagcctgtgc aggagtatct ggcattatct
    10381 ttcctaattt agtcgttgga gaacctatat acaaaaatac tggaactaaa agtattgctg
    10441 ctaatattat attgaatatt ggtcctgcta caatagtact aaatctttgt aatatagact
    10501 tgtttccaaa agaatttgga cttatttgtt cttcatcttc accttccatg ctgacatatc
    10561 caccaattgg aagtgctctt atagaatact ctgtatcttt ttttacacta tatatcttag
    10621 gccccatacc tatagaaaac tcatttactt tgataccact tctttttgca aatataaaat
    10681 gtcctaactc atgtattaat acaattatac taaatagtat taatgcggct attatagtca
    10741 taaattcacc ttcctatttt tttatcagat ttgcaacata agctctactc catctatctg
    10801 tttctaagat ttcttctaat gttggttcac ttatgctact atgaacttct aaagtcttct
    10861 ctatgtaata tggtatgtca tagaaaccaa ttttatcctc taaaaattca cttactaaaa
    10921 cttcatttgc tgaatttaaa acagaggaat aagttcctcc catttttaaa cactcatatg
    10981 ccaattttaa acatggaaaa gtttctaaat ctggttcttc aaaagttaaa gtactaaatt
    11041 ttgaaaaatt cattctttca aagctacttt ccattctatc tggatatgta agagcatact
    11101 gtattggtag tctcatatct ggacatccta attgagcaat tattgagcta tctgtatact
    11161 gcaccattga atgaattatg ctttgtgggt gaactactac atctatattt tcttgttcta
    11221 ctccaaataa ccatctagct tctatcactt caagtccttt attcataagc gttgaagaat
    11281 caatacttat ttttcttccc atactccaat ttggatgctt caaagcttca tttttagtta
    11341 tatttgcaag ttctcccttt ttctttcctc taaatggacc accagatgct gtaagtatta
    11401 tcttttctat atttttttta ttttctccat ttagacattg aaatatagca ctatgttcac
    11461 tatcaacagg aagaatattt acattgtatt tttctgcttc tttcattaca agttcaccag
    11521 cagttaccaa cgtttcttta ttcgctaatg ctatgtctat tcccttcttt atagcacaaa
    11581 gagttgggac taatcctatc atacctacta cagcagttaa aaggacatcc acttcatcaa
    11641 gtgatgatat tatttttaat ccttccattc ctgctaaaac ttctatttct atatttttag
    11701 gcaacatttc ttttaatttc aatgctttat tttcctcaaa aactgttaca tatttaggtt
    11761 taaactctac tatctgttct agcaataatt caatattact gttagctgat attgcaacta
    11821 tctcaaactt atctctatta tctctaacta catccaaagt ttgtttacct atagagcctg
    11881 tagaacctaa aattgatatc tttttcatat aataatcctt tctaataata aatataccct
    11941 gtcaaaacat tataacacta ctaaaacaat tatttttact attcatagaa atttcatata
    12001 ttattccaca gattaaaatt aatctattat tttttgtatt ttgtggaata ttataaaata
    12061 ttctagtatt attaaatgct cttttttatt tccacaacat cttctatttt acagtctagt
    12121 gctaaacata tcttctccat tgtatccata gaaacagatt tatcatttcc tagtctgcct
    12181 aaaacagaaa tacttatacc tgatatattt acaaattcca ttttctttat ttttctgtca
    12241 attaacaatt tccataatgg agcgtatgaa atcatgttat cacctcgcaa aatatattct
    12301 atcacatttt atctattttg gaaaattttc atctatttag ataaataatt atttatctaa
    12361 atagatttca attatagttt attaaatata aatatgttaa aataaaacaa aagcataagt
    12421 tcaaaaatta aactt
//
LOCUS       DAMJVI010000102        12204 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.107, whole genome shotgun sequence.
ACCESSION   DAMJVI010000102 DAMJVI010000000
VERSION     DAMJVI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.107"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(38..2149)
                     /locus_tag="PC545_002971"
     CDS             complement(38..2149)
                     /locus_tag="PC545_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457723.1"
                     /translation="MTINKLYSKLRAYNWKNYTTLFFCIILSVVLVTSYALIYFSPTI
                     QNILPTGGDSRKQATMIFSVAILGCTVFTTYASSLFLKFKSKEIGIFMALGTDKKKLK
                     KLLFSEILLISVVCCFLGLILSIPVSFGIWKIFQLVIIDTREMTYHFGTQGFIYGFIF
                     SVFVTLCIFILSVKFINRTNIIDILNDQRISEPIQDVKDWYGILGIIFIILGIFLGYL
                     VPSLTINKLNYRLPSVWSFTYLLSLIGIYMIMTYIVVHAKRGKHPEKYYKNIISTSMM
                     RFMGRQTVKNMCVISFLIAGALFAVFYVPNMVTGIYDTLKNNPIDYTFTYNQRDKQVS
                     KKDIEQLADKHNIKIQNYMEIPSIELIVDGVESIYHKDGTIEKVYMKKSSYDMFFNSK
                     DFSKLIGKPITIKNGEYLAVVNKGNENQDYKYTKFISSPVTEKEIPFRYAGKIIFNGS
                     FFQNRSMNAYVLNDEDYKSFTKDLSIKNYNNSILFNVDKDTYAFANDLKKEIIKRTSK
                     EMGMFSGYDEYVKKRYESTGRKYFMDEEYPSGEGHLKLDPNNNQLYLNWKYYPNFKIL
                     QSQDMIKNMAVFLMLFIYVAIICFTAVGIIAYTRGVTIAINHKQVFMDLNRLGANNDY
                     IKFCIRNQLKKVFSYPLIAGSFLIYLFNFMIMLNNDGRIVNSEVNALLINLGFIGICG
                     VYMYLIYLLTYKKFKKIIGIL"
     gene            complement(2139..2891)
                     /locus_tag="PC545_002972"
     CDS             complement(2139..2891)
                     /locus_tag="PC545_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457724.1"
                     /translation="MLKVNQLCKSYKTGSKTYPVLKNISFEINKGEFVAIMGSSGSGK
                     TTLLNCISCFIPIDNGEIILGNNNIRNLKEKELSEVRNQQLGFVFQDFMLFDGLSVFE
                     NICVPQIIKGSQVLQMENRAINLCELFGIEHIRKKYPAEISGGEKQRTAVARALMNNP
                     DLILADEPTGNLDSKSCRTVIESFLKAKQELDATILMVTHDSFAASFCDRVIVLKDGI
                     IYKEVVKSNSRHKFLDELLEVLKEMGGAQDDN"
     gene            complement(4085..4261)
                     /locus_tag="PC545_002973"
     CDS             complement(4085..4261)
                     /locus_tag="PC545_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457725.1"
                     /translation="MFFTFIGVISAILLLCTIICGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(4365..4973)
                     /locus_tag="PC545_002974"
     CDS             complement(4365..4973)
                     /locus_tag="PC545_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2457726.1"
                     /translation="MKKLLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINISIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENQNNKKTEAEYRTIAYQITSF
                     IQLLFLRSNAFFKYSGIDILNVEQRNGLIDFQLDLLLVDITK"
     gene            complement(5072..5896)
                     /locus_tag="PC545_002975"
     CDS             complement(5072..5896)
                     /locus_tag="PC545_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2457727.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAKTDKYTGHRLYSVE
                     QISILQRIVLLRDSKFSVAEIANIVHNWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISLREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQSNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHKGEHNEINPENYLTEIQIPLIKV"
     gene            complement(6074..6967)
                     /locus_tag="PC545_002976"
     CDS             complement(6074..6967)
                     /locus_tag="PC545_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2457728.1"
                     /translation="MQLYNTPFNTNKHLIYFFTFTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEIRIKWILLTILFFALTFIT
                     SLIIGNYFNVKTSDIDWIHIAVFRPSKIAYMLLASFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFNIQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDW"
     gene            complement(7224..7661)
                     /locus_tag="PC545_002977"
     CDS             complement(7224..7661)
                     /locus_tag="PC545_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2457729.1"
                     /translation="MKIHVEQGINCDDIEITIKCSALDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVYDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRIQATLKNNEKVIINRRYIKNIKEKLNI"
     gene            complement(7918..8067)
                     /locus_tag="PC545_002978"
     CDS             complement(7918..8067)
                     /locus_tag="PC545_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HDF2457730.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LIFKK"
     gene            complement(8080..8310)
                     /locus_tag="PC545_002979"
     CDS             complement(8080..8310)
                     /locus_tag="PC545_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HDF2457731.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIE"
     gene            complement(9169..9390)
                     /locus_tag="PC545_002980"
     CDS             complement(9169..9390)
                     /locus_tag="PC545_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HDF2457732.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVVKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            9970..10227
                     /locus_tag="PC545_002981"
     CDS             9970..10227
                     /locus_tag="PC545_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2457733.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVAKLYGGTNTYIPTYKGL
                     FRYARNRDIVKQFNGVNHNELAIRYNMCVSNIKRILNENSV"
     gene            10696..11934
                     /locus_tag="PC545_002982"
     CDS             10696..11934
                     /locus_tag="PC545_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDF2457734.1"
                     /translation="MNKGFYKLNSVVLNFDIKNDVNKIRFNHYIMRKENIQRLQGTLP
                     NGQFYMTVRRAATDLQLSISTISRLVKEFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKYMELDCVCNDEFIDNSGIEFDCDSKDNSFNGDINVETVHCINEYFSDDDVE
                     HNYNNFYNKDTMKDKAISNISTIKKYIDESFDTNKSENSNLQQESINFNFNDTCNEID
                     KRTNNSIENNQNKRPMRLSDISVLSDKGMTNKKNYFNHNHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNVHPCKEGNSNDSSNYIHSDYTTYDG
                     SVRERKKYENVRYWNPICEI"
BASE COUNT         4518 a         1445 c         1649 g         4592 t
ORIGIN      
        1 tgattttatt actttaaaaa tctaataacc ttttttatta taaaatacca ataatttttt
       61 tgaatttctt ataagttaat agatatataa ggtacatata aaccccacaa atccctataa
      121 aacctaagtt tatcaaaaga gcattgacct cactattgac gattctacca tcattattta
      181 acataatcat aaaattaaac agataaatta aaaaacttcc tgcaataaga gggtatgaaa
      241 atactttctt taattgattt ctaatacaga actttatata atcattgttt gctcctaatc
      301 gatttaaatc cataaagacc tgcttgtgat ttatagctat agtaacaccc cttgtatatg
      361 caatgattcc tactgctgta aaacatatga ttgccacata tatgaataac attaagaaga
      421 ctgccatgtt ttttatcata tcttggcttt gtaaaatttt aaagttagga tagtatttcc
      481 aattaagata caactgattg ttgtttgggt ctagttttaa atgaccttca ccacttggat
      541 attcttcatc cataaaatat tttcttccag ttgattcata acgttttttt acgtattcat
      601 cataaccact gaacatgccc atttccttag atgttctttt tataatttct ttctttaagt
      661 cattggcaaa agcataggta tctttatcta cattaaatag tatagaatta ttgtaatttt
      721 ttatggataa atctttagta aatgatttat aatcctcatc attaaggaca taggcgttca
      781 tacttctatt ttgaaaaaaa gaaccattaa atattatttt tccagcatat ctaaagggga
      841 tttctttttc tgtgactggg cttgatataa atttggtata tttataatct tgattttcat
      901 ttcctttatt tacaacagca agatattcac cattttttat agttattggt ttgccaatta
      961 gcttggaaaa atctttagaa ttaaagaaca tatcatagga gctttttttc atatatacct
     1021 tttcgatagt tccatcttta tgatatattg attctacacc atcaacaatt aattctatag
     1081 aaggaatttc catatagttt tgaattttta tattgtgttt atctgctaat tgttcaatat
     1141 ctttttttga tacctgttta tctctttgat tgtaggtaaa agtataatct atagggttat
     1201 ttttaagagt atcatagata cctgtaacca tattaggaac atagaataca gcaaataatg
     1261 ctcctgcaat caaaaatgaa ataacacaca tgttttttac tgtttgtctt cccataaatc
     1321 tcatcatgct agttgatatg atgtttttat agtatttctc tggatgtttt cctcttttag
     1381 catgtacaac tatgtatgtc ataatcatat atattccaat aagagagagt agataagtaa
     1441 agctccatac agatggtagt ctataattta atttgtttat agttagtgaa ggtacgagat
     1501 atcctagaaa aatacctagt ataataaaaa ttatacctaa tataccatac cagtccttta
     1561 catcctgtat tggttcagat attctctggt catttaatat atcaattata tttgtacgat
     1621 taataaattt tacagaaagt ataaatatgc ataatgtcac aaatacacta aatataaatc
     1681 catatatgaa tccttgagtt ccaaaatgat atgtcatttc tctggtatca ataatcacta
     1741 attgaaatat tttccatata ccaaaagata caggaatact taatataaga cctaaaaaac
     1801 agcatactac tgaaattaac aatatctctg aaaatagaag tttttttaat ttcttcttat
     1861 ctgttcctag agccatgaaa ataccaattt ccttgctttt aaattttaaa aagagggaag
     1921 atgcgtatgt agtaaagaca gtgcatccta atatagcaac tgagaatatc attgtagcct
     1981 gttttctgct atctccacca gttggaagta tgttttgtat tgttgggcta aaataaatta
     2041 gagcatatga agttactaat accacagaaa gtattataca aaagaataaa gttgtatagt
     2101 tcttccaatt ataggctcga agtttactgt ataatttatt aattgtcatc ttgagcgcct
     2161 cccatttctt ttaaaacttc tagtaattca tctaggaatt tatgtcttga atttgatttt
     2221 acaacttctt tataaattat tccatcttta agcacaataa ctcggtcaca gaagcttgct
     2281 gcaaagctat cgtgtgtaac catgagtata gtagcatcta attcttgctt tgcttttaaa
     2341 aatgattcaa tgactgtacg acaagactta ctatcaaggt taccagttgg ttcatctgct
     2401 aatattaaat ctgggttgtt catgagtgct cttgctactg ctgtacgctg tttttctcca
     2461 ccagatattt cggcagggta tttttttctg atatgttcaa taccaaatag ctcacataga
     2521 ttaattgcac ggttttccat ttgtaagact tgactacctt tgataatttg tggaacacat
     2581 atattttcaa agacactaag accatcaaat agcatgaagt cttggaatac aaatcctagt
     2641 tgttgatttc taacttctga aagttccttt tcttttaaat ttcttatatt attatttcca
     2701 agaattattt ccccattatc aattgggata aaacatgaaa tacaatttaa aagagtagtt
     2761 tttccacttc ctgatgaacc catgattgcg acaaattctc ctttgttgat ttcaaaagaa
     2821 atatttttta atacaggata tgttttactg cctgttttat atgatttaca taattggttt
     2881 actttaagca tatatatcat cctcactttc atttatcttg taagtcaatg atagcatttg
     2941 aaaggggagg agggatatga gctatattgt aatagttgga tgtttttttg taatgtttga
     3001 aaatatattg tttggatagt gtgagaggtt attattatag ttaaattgat tatattattg
     3061 gaggatttag agggttattt ttaggtatta attgttgtgc taaagtaata tgatgtggaa
     3121 cattgaaggt gtagttagaa aagaagtata tagtaaaagt aaaaaggtta tagaataacg
     3181 aatgttgtat tgaaaaaaat gatgtaaata atgtatagta gggttaaaaa gaaaaaaaga
     3241 attatagagc agtaagatta tttaatggaa tgtattgtaa ataatttgaa aggatttagg
     3301 ggctattaac acaatgtaat tgagaaaata tattggagtt ttggaaataa ataggttgtt
     3361 aaaaacaatt gaaatttata ataaaaaggt taaaaagtat tattagtata ggtatgatag
     3421 ataattttag aaagtggaag tattttagac tatgctgaaa agtgattgat atgattttga
     3481 gatattaaaa tattactata ttatagtaaa gaagttaaaa taataaaatg ttgtaataat
     3541 aaaggtttgt agtataataa agaactgtaa taaataagac tttgcaatgt aatagaaatg
     3601 ctgtaataat aaaggtctgt agtatagcaa agaactgtaa taaataagag attgtaataa
     3661 taaaagtctg tagtataata aagaactgta ataaataaga ctttacaatg taatagaaat
     3721 gctgtaataa taaaggtctg tagtataata aagaactgta ataaataaga ctttacaata
     3781 taatagaaat gttgcaataa caaaatacca taataaataa gagtttgcaa tataatagaa
     3841 atactgtaat aacaaaacac tgcaataaat aagagattgt aatataacaa aatcctataa
     3901 taaataagat tttacaatat aataaaaatc ctataataaa taggactctg taatataata
     3961 aaatgctgta acaacaaaac actataataa ataagaccct gtaatataat aaaaatacta
     4021 taatatagta aaaatcatgc aataataaaa atctataata ataaacctca gcataacaaa
     4081 gacattaatt tcttactaaa aaggccacca caattaacag tgaaaaagca attgaaagta
     4141 ttgaaaaagt aaagtgagaa ttagagtcat tattacgatt attaagtttt atccataaac
     4201 cacatataat tgtacacaac aataaaattg ctgatattac tcctataaat gtaaaaaaca
     4261 ttaaaattcc tcccccttat ttaatgcaag tatttttagt caatgagaat attttcaaac
     4321 gttaccataa aataaaaaaa tgtacataaa aaattaatat agtattattt tgtaatatct
     4381 accaataata aatctaattg aaaatctatt aaaccatttc tctgttcaac attaagaata
     4441 tcaataccac tatacttgaa aaaagcatta gaacgaagaa aaagcaactg aataaatgaa
     4501 gttatttgat acgcaatagt tctgtattca gcttcagttt ttttgttatt ctgattttca
     4561 tagtgagcct taattaaagg tagtatctca aatggataaa caatttcttc ctgtatcaat
     4621 atatagggta cagaaatctt tataaattga ctatgttcac ctataaaatc aaaaaatgca
     4681 attagtattt cccttaattg ctgtttaggt gctaaatttt tagtatcatg tgtagcctta
     4741 tattcatcca tatgacgctt taaaaatttt ttaatagaaa tattaattaa ctcctcttta
     4801 gatttaaaat aataattaat catagctaaa ttgacatttg ctttagctac aatttctcta
     4861 gccgtaatgc ttgaaacatc tctatgtgaa gatagtaact ggattgtggc attaattaat
     4921 tgttcctttg ttgaatctgc tttcataatt tttcccctta aaagtttctt cataaaaaaa
     4981 ttaaacatgt ttaatacacg tttagatact atcatgaaaa ttttgggatg tcaatgggtg
     5041 attagtactt aagtatttaa gtactaatcg attagacttt tataaggggg atttgaattt
     5101 ctgtaagata attttcagga tttatttcat tatgttcacc tttatgaccg atttgtctat
     5161 ttatacctga tatttgataa tcactatttt tatctaacca ataacagaac gattcatatg
     5221 caccagctat attttcatat ggaccataaa ccatagtaca tgccatcata tcaatttctt
     5281 ctgtctcacg atatataaat ccagatttgt tctttccaat cttctttaca accattccaa
     5341 cttcaatatc tacaccatta tctttgactt cttcatcatg gtaaaaagct atgttattgt
     5401 tggattgacg tgatacttct atatgttctt ctttgataaa tttagatagc ttttcccaga
     5461 gtataccttc agattggtag tctggaatta tctctctaag tgatatgatt ttataacttg
     5521 gaatcttttt aaagacaaca ttgtaatgaa tttcatcttt atcacagttt atagcttcta
     5581 taaatttatc aattttattt attctttgtt gttcctgctt tatttccttt tgtatttcat
     5641 tcttttttct attcaattcc ttaattacaa attcatcatt ccaattatgt acgatatttg
     5701 ctatttctgc aacgctaaat tttgaatctc tcaataatac aattctttgt aatattgaaa
     5761 tctgttcaac tgagtataat ctatgacctg tatatttgtc tgtttttgca ggttttaaaa
     5821 taccaagttc atcataataa cgcaacatac gaatagaaat ttgtgttagt tttgaaacct
     5881 ctcctatttt aaacatgaaa atatgacctc cttttcactt ataatcagta tatattttgt
     5941 ttttatatac ttcaagcgtt tgtggaaata caattatttt ccagatgtta caataaatta
     6001 tagagagtaa attatattta agtagtaatc gtatcaaatc atagattatc ttaatttata
     6061 attcatatta aaattaccag tctaactcaa attcaaaatc atccgacttt atctgttcaa
     6121 tttcagaatt taccttctgt ttaaatttat tagaagaaat catatataca agaaatatta
     6181 aaccaaatat tatttgtaca tattgagcta ttacacataa tttaataggg tagtgtagaa
     6241 gtaattgaat gttgaaaaaa tagtacatca tatgaactaa cccagctgat aaaatgcttc
     6301 tacttgtatt gatatataca aaattaatta taaagtttaa aagtacaatg tttggtataa
     6361 ataaaaaagc atcaaatagg gagtattgtg ccaaattata tggtatttga ttaggtgtaa
     6421 aatacgaccc tagataccag attccacaaa caagccctaa aataattgaa gagcctataa
     6481 atccaaatct aactaataat ttgtcaatgg catatcctct ccatccagct tcttgactaa
     6541 gtatactcaa tataaaactt gctaatagca tataagctat tttagaaggt ctaaacactg
     6601 ctatgtgtat ccaatctata tcagaagttt ttacattaaa gtaatttccg attattaacg
     6661 aagtaataaa agttaaagca aaaaataata ttgttaatag tatccatttt attctaattt
     6721 ctttaaagct taggtatcta taaataaaat cttgttttgc actttgtgga tagattgtag
     6781 aaataaaaaa tagacctaca atagctggtg atgtatatgc aagataaaaa atgatttctc
     6841 caagtattgt atttgtaatt ccgagcaacg caggtgtaag actgaatatc cagttcaata
     6901 ctagcgtaaa tgtaaagaaa tagattaagt gtttgtttgt attaaagggg gtgttatata
     6961 gttgcatttt ttcactatcc ttccgtttta taatgaagtt ttaattttta ttaattgttt
     7021 atttattttt atatgtatta agttatcgct tgatgttagt tattgcctat aataatatcc
     7081 tattttttat attttgatta attattttta cactaaatat aattaattag tatttaatat
     7141 tgaatatatt atattattat taattattaa ataggtcaat aaatttgcaa ttagtgatat
     7201 taataaatta ctttagattt ttattaaatg tttagctttt ctttaatatt ttttatataa
     7261 cgacgattta taataacttt ttcgttgttt tttaaagtag cttgaatgcg attatgtgat
     7321 attactttta tactttttat gtagtccata tttaaaatca tggatttaga tattcgtgag
     7381 aaggatgtat tagctaataa ttttgataat ttatatattt ttaaatcaat ttcaaataca
     7441 tcatcttcat aataagcaaa tgttttattt ccaactgtct caaagtataa aatatcatag
     7501 acattgagtt tataaatctc tcctgatttt ttacctttta tatcaagtaa cggtgtactc
     7561 aataatgaaa ttattttttc aatgttatta tctagagcag aacattttat ggttatttca
     7621 atatcatcac aattaatacc ttgttcgaca tgtattttca ttaatcatca cctcatttta
     7681 ttactgtcat tagaatagca ttatatctta ttgagtccaa ctagatgcgt tctaaatttc
     7741 aaattaagca tagtgaaatt caaaaataaa acagtgaaat tcaaatttga ggtggtaaaa
     7801 ttcaaaaaaa tactatggat atttggataa attatccaaa tatcctagca ttttacatag
     7861 aatatgccta attattttac aaatagacct attttatgac ttacatatat tgtcaaatta
     7921 cttcttaaat atcaaaatat ttttggataa tgtatcaata ctgtttgata aagtatttaa
     7981 tttaccctca attctaacca ataagtacat ggatagagct ataggaaaac caacattggt
     8041 tattaacagt tgtaaactat catccataaa attagccccc tattctataa ctaagtcata
     8101 aggagtggta tttgtttcca caaccttggc ttcaaccaca gaagctaggt caaatcctcc
     8161 aaccgaaaaa atatttttag atactatcaa atccatgaca gattttatat tttcttcagt
     8221 taaatcatcc tttggatttt ctatagaaaa agaaacttgt ttatccttta catttttaaa
     8281 gctcatgact aattttttct taatttccat ataattacct ccgatataat aatttatatt
     8341 ttaattgttt gcttgttaat attggtattt taattgtcaa tattagtgtt ttgcttgtta
     8401 gtattaatat ttcaattgtc aatattaatg ttttgcttgt tagtattaat atttcaattg
     8461 tcaatattag tgttttgttt gttagtatca gtgtttcaat tgtcaatatt agtgttttgc
     8521 ttgttagtat tgatatttca attgtcaata ttagtgtttt gcttgttagt attgatattt
     8581 caattgtcaa tattaatgtt ttgcttgtta gtattaatat ttcaattgtc aatattagtg
     8641 ttttgcttgt tagtattagt atttcaattg tcaatattag tgttttgctt gttagtatta
     8701 gtatttcaat tgtcaatatt aatgttttgc ttgttagtat tagtatttca attgtcaata
     8761 ttaatgtttt gcttgttagt attagtattt caattgtcaa tattagtgtt ttgcttgtta
     8821 gtattagtat ttcaattgtc aatattagtg ttttgcttgt tagtatcagt gtttcaattg
     8881 tcaatattag tattttaatt gttgactcta gttattgctg atatagaaaa tatttaattt
     8941 atttcaatta attagattaa agtagggtag catctaaaat agctaacatg tttaagttga
     9001 aatacgttta atatattaat ttttcaaagc gttaatatat taaagttata accaaaattg
     9061 aagttataat ctgccaaatc tatagcgtat taagtacgtt atataactta atcttttata
     9121 taaagagtta attttttaat ttgtaaagca gagtacgttt aaaagatact atttagatag
     9181 tgatgtacta tctaatttta aaatatctac aatagggttc tcttgaagac ttgctattgc
     9241 ttctgcaaca gcataaacat cttcatttaa tgcctctgct tttacatttg caaatgattt
     9301 acttttaaca actgccttac ccttatcatc aaaaccagca tccaatttta gttttatact
     9361 agataaattt tttgttgaaa caacttccat aaaattacct cctaaaaatt taatttcaaa
     9421 gatttgtcga ctttctttgt tagctataat actatcatct aagataatat cggagttaaa
     9481 cagtaatttc taaagatttt ttgtgtaaat ttttatagga aaaattttac ttttttgctg
     9541 atgaaaaagt ttacaagtta tttgtgaaga tatgttctta gtgttttgaa atagtatttg
     9601 tatatattcc tgcttagttg agatagtttt gtatggattt tcatatgtgt agagttaaat
     9661 gttaggtact aaagtttatg ttaaattaaa tgttaaagct tatgttaaaa attttattaa
     9721 atttaatctg taaataaaaa agttaaaagt ccaatattaa cattttactt ttaaaccatt
     9781 aaaatcttta ttaaagctaa caaagttata agttatgaga aattttattg aagttaatac
     9841 aaaaattaat tttaaagcta agaaattatt tattagattc aataaaacta ataaatatag
     9901 tttagtcaat aatgtattaa ataaaaactt tgttaagtct ataaaaatat cttaaaggag
     9961 gtgtaactta tgaagataaa agtggaggat ataccagttc aatttcatgc aatggtagaa
    10021 ataattggaa ttgataagtt tatagaggtt gccaagttgt atggaggaac aaatacttat
    10081 atacctacgt ataaggggct ttttagatat gctagaaata gggatatagt aaaacaattt
    10141 aatggagtca accacaatga attggctatt agatataata tgtgtgtaag caatattaag
    10201 cgaatactca atgaaaatag tgtttaaata taataaaata atgtttaaac ataatgaaaa
    10261 tagccttgtt aaacgtaaga gtaaagacaa agaagatgtt ttataaagtt tttttagaca
    10321 aattaactaa atttactact tattaattta aatacattgt atattctata aaaaaatttg
    10381 ttaaatactg gagatatatt gagaatacaa agaaaatggt cgaaaatatt tgttggctaa
    10441 aaaatagata aaatgataca attgcttgta taaaggggaa aaataaacaa ataaactata
    10501 taaataaaga aatagcaatt aatatgacat aatatattta taaaattaag aatataaata
    10561 gatattttaa ggtattagtt gttaaattag gaaatattaa aaaaaatata tatttatatt
    10621 taaactattt tgttatttat ataaaaatac ttatctagtt actagcaatt attagtagaa
    10681 atgggggaaa tagagatgaa taagggattt tacaaattaa attcagtggt attgaacttt
    10741 gatattaaga atgatgttaa caagataaga tttaatcatt atataatgag aaaagaaaac
    10801 atacaaagac ttcaaggaac tctgccaaac ggacaatttt atatgacagt gagaagagct
    10861 gctacagatt tacaactttc tatttctact ataagtaggc ttgtaaaaga gtttttggat
    10921 ttagggataa taagattagt gtctaagggt gtaaaaggta atcgttctgt atatagttac
    10981 gtaagtatag agtttaatgg aagtttattt gatgatagta aatatatgga acttgactgt
    11041 gtatgtaatg atgaatttat tgataatagt ggcatagagt ttgattgtga tagcaaagat
    11101 aattctttta atggagatat aaatgtagaa actgtgcatt gcattaatga gtatttctct
    11161 gatgatgatg tagagcataa ttataacaat ttctataaca aagatactat gaaggataaa
    11221 gccatttcta atattagcac tatcaaaaaa tatattgatg aatcttttga tacaaataaa
    11281 tcagaaaatt ctaacttaca acaggagtca ataaatttca actttaatga tacatgcaat
    11341 gaaatagata aaagaacgaa taatagtata gaaaataatc aaaataaaag gcctatgaga
    11401 ttgagtgaca tcagtgtttt aagtgataaa ggtatgacaa ataaaaagaa ttactttaat
    11461 cataatcatg gtatacaaaa agggactaaa tctgtaacaa agaaaaaaga atccttaaat
    11521 aaatttaata aaaaaaatat acagcaaagc attataaata tgctaaataa aaaatcaggt
    11581 aagaatttta aaatcgattc tccaataagc ataaaactta tcaatgaaag gctaaaggaa
    11641 ggctatgtct tagaagattt ctacaaagtt attgaggtaa aagtggctaa gtggaaaaat
    11701 accataatgg aaatgtatat aagaccagaa accttgttta gctataagtt tgaatcctat
    11761 gtaaatgaaa acatatctat gaaaagtcaa agtaatgttc acccatgtaa agaaggcaat
    11821 agtaatgata gcagtaatta tatacattct gattatacaa catatgatgg cagtgtgcgt
    11881 gagagaaaaa aatatgagaa tgtaagatac tggaatccta tatgtgaaat ttaattagtt
    11941 taagaatgta tttatgataa ataagatatt aatagagaat agtgagctat agcaaatgat
    12001 atatcaatag ttggtagtaa gttatattaa gcaagatatt actatcaggt agtgaattgt
    12061 attgaacgat atatcaataa ttggtagtaa attatattaa gtaagatatt actatcaggt
    12121 agtgaattat attgaacgat atattaataa ttggcagtaa attatattaa ataagatatt
    12181 actatagagt aatgaattat attg
//
LOCUS       DAMJVI010000103        12149 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.108, whole genome shotgun sequence.
ACCESSION   DAMJVI010000103 DAMJVI010000000
VERSION     DAMJVI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.108"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            153..941
                     /locus_tag="PC545_002983"
     CDS             153..941
                     /locus_tag="PC545_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2457735.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEGKIDILVNNFGSTNPSLDKDL
                     VTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPEFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
     gene            1412..5128
                     /locus_tag="PC545_002984"
     CDS             1412..5128
                     /locus_tag="PC545_002984"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDF2457736.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIVKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            5170..8655
                     /gene="rpoC"
                     /locus_tag="PC545_002985"
     CDS             5170..8655
                     /gene="rpoC"
                     /locus_tag="PC545_002985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDF2457737.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNENIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKALGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREIDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            8944..9366
                     /gene="rpsL"
                     /locus_tag="PC545_002986"
     CDS             8944..9366
                     /gene="rpsL"
                     /locus_tag="PC545_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDF2457738.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            9490..9960
                     /gene="rpsG"
                     /locus_tag="PC545_002987"
     CDS             9490..9960
                     /gene="rpsG"
                     /locus_tag="PC545_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDF2457739.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            10009..12075
                     /gene="fusA"
                     /locus_tag="PC545_002988"
     CDS             10009..12075
                     /gene="fusA"
                     /locus_tag="PC545_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2457740.1"
                     /translation="MARKFPLERTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFVNKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4702 a         1533 c         2498 g         3416 t
ORIGIN      
        1 ttttacattt tagtttaaaa aaatacacga atgttttata aataaaatgt aataaataat
       61 taattaacaa atataaattt taaaataagt tagatattta gaaaaatcga aatttgtagg
      121 atttaaagga catattcaag gaggagttaa taatggaaaa attacaagga aaaattgcag
      181 tagttactgc agcaacaaaa ggtattggat tagcatcagc agagatatta gcaaaaaatg
      241 gagcaactgt gtacttagca gctcgttcag aagaattagc taatgaagtt ataaataaaa
      301 taagtgcaga aggtggttgt gctaagtttg tttactttaa tgctcgtgaa gaagagacct
      361 ttacctcaat gatagaagag gtagttaaaa aagaaggtaa gatagatata cttgtaaaca
      421 attttggttc aacaaaccct tctcttgaca aagaccttgt aactggggat acagataatt
      481 tctttgacac agtaaatact aatttaaaaa gtgtgtattt accatgtaaa gcagcaattc
      541 ctcatatgat aaagaatgga aaaggaagta tagtaaatat atcaagtata ggttcagtat
      601 tacctgattt atctagaata gcttactgtg tatcaaaagc agcaatcaac tcattaactc
      661 aaaatatagc tacacaatat gctaaagaca atgttagatg taatgctgta cttccagggc
      721 ttattgcaac taaagcagca ttagacaata tgtctccaga gttcataaaa gaatttttaa
      781 agcatgttcc tttaaatcgt ataggggaac cagatgatat agcaaaagct gttctattct
      841 atgctagtga tgactcatca tttataacag gggatttact tgaagttgca ggaggatttg
      901 gtttacctac tccacaattt gcagataata tattaggata attaaattac taatatgcaa
      961 tgaaagctta tgcattagtt atagtagaaa tgatattttt ataaaggcca tagatataat
     1021 ttatattgga aaggtttgag gttcaaaatt ccaagttaaa tttatttatg gtctttattt
     1081 tgtatatttt aataaattgt atatatttgt ataaatgtaa ttatttacaa tcattttaat
     1141 atggaattat aattaaatag gggggatggt acttgtataa aattttttat agaaaattag
     1201 acctataaat gaaaaatatg gattttatat tggaaaattg tacataatac ataatatatg
     1261 cttaaaataa tttacaaaaa atcaaaaata atgcatagtt tacatataag ataaaaaata
     1321 tattgaaaaa tacttgacat tatgctaaaa ttattagatg cgttagaatg aactattgtt
     1381 ttatttttaa ttattgagag gtgaaaagtg aatgccacat cctgtcacga taggtaaaag
     1441 aactagaatg agcttttcta aaattaagga aatagctgat gtgccaaatc ttatagaaat
     1501 tcaagtagat tcctatgagt ggtttttaaa agaaggttta aaagaagtgt ttgatgatat
     1561 ttcaccaata gaagattata ctggtaatct tatattggaa tttgtcgact attctctaga
     1621 tgataaacca aagtacgata tagaagagtg taaagagaga gatgctacat attgtgcacc
     1681 tctaaaggtt aaggtaagac ttataaataa agaaacaggt gagataaaag aacaagaagt
     1741 gtttatgggt gacttccctt taatgacaga aagaggaact ttcgttataa atggagcaga
     1801 aagagttata gtaagtcaat tagttagatc tcctggtgtt tactatgcgg aagaaagaga
     1861 taaaacaggt aagagattga tttcatctac tgtaattcct aatagaggag catggttaga
     1921 atatgaaaca gattctaatg atgtaatatc tgtaagagtt gatagaacaa gaaaacaacc
     1981 agttacagtt ttacttagag ctttaggaat aggaacagat gcagagataa ttgacctttt
     2041 aggagaagat gagagattat ctgctacatt agaaaaagat aatactaaga cagtagaaga
     2101 aggtcttgta gagatataca aaaaattaag accaggtgaa cctcctactg tagaaagtgc
     2161 atcatcttta ttaaatgctt tattctttga cccaaaaaga tatgacctag caaaagttgg
     2221 tagatataaa ttcaataaga aacttgcttt atgttacaga ataatgaata aaatttcagc
     2281 agaagacata ataaatccag aaactggaga agtatttgta aaagctggag aaaaaataag
     2341 ctatgatttg gctaaagcca tacaaaatgc aggtataaat gtagtaaatc tactaatgga
     2401 tgatgataaa aaagttagag ttataggaaa taattttgtg gatattaagt ctcacattga
     2461 ttttgacata gatgatttaa acataaaaga aaaagtacat tatccaactt taaaagaaat
     2521 tttagatgga tatagtgatg aagaagaaat aaaagaggct ataaaatcaa gaattaaaga
     2581 acttatacca aaacatatat tattagatga tataatagct tcaataagtt atgagttcaa
     2641 tatattctat aatataggaa atattgatga tatagatcac ctaggaaaca gaagaataag
     2701 atctgtaggt gaattgttac aaaatcaagt tagaattggt ctttcaagaa tggaaagagt
     2761 tataaaagaa agaatgacgg ttcaagatat ggaagctata acgcctcaag cgttagttaa
     2821 cataagacca gtttcagctg caataaaaga attttttggt agttctcagt tatctcagtt
     2881 catggatcaa acaaatcctc tatctgagtt aacgcataag agaagattat cagcccttgg
     2941 gcctggaggt ctttcaagag aaagagctgg attcgaagtg cgtgacgttc accattcaca
     3001 ctatggtaga atgtgtccaa tagagactcc agagggacca aatatcggtc ttataaactc
     3061 tctaggaact tatgcaaaaa taaatgaatt tggatttata gaatcaccat atagaaaatt
     3121 tgataaagaa acatcaacag ttactgatga aatacattat ttaactgctg acgaagaaga
     3181 tttattcgta agagctcagg caaatgagcc attaacagaa gatggtaaat tcgtaaacca
     3241 tagagttgtt tgtagaactg taaatggtgc tgttgaaatg gttcctgaaa gtagagtaga
     3301 ttacatggat atatctccta agcaggttgt atctgttgcc actgctatga tacctttctt
     3361 agaaaatgat gatgccaacc gtgcccttat gggagcgaac atgcagcgtc aggcagtgcc
     3421 tctagttaga agagaagcac caattatagg aactggtata gaatacagag cagcaaaaga
     3481 ctctggagca gttgttgttg ctagaaattc tggtatagca gaaagagtaa cagcagatga
     3541 aataattata aaaagagaag atggaaacag agatagatat aatctactta agtttaaacg
     3601 ttcaaactca ggtacttgta taaatcaaac acctataata aataagggtg accagatagt
     3661 aaaaggtgat gttatagcag atggtccagc aactgattta ggagaagttg cacttggaag
     3721 aaactgtctt atagcattca tgacttggga aggttataac tacgaggatg ctatattaat
     3781 aaatgaaaga ttagttaaag aagatagatt atcaacaatt catatagaag aatatgaatg
     3841 tgaagctaga gatacaaaac tagggccaga ggaaataact agagatatac ctaatgttgg
     3901 agatagtgca attaagaact tagatgacag aggtataata agaataggtg cagaagtaga
     3961 ctcaggtgat atattagttg gtaaagtaac tccaaaagga gaaactgaac ttactgctga
     4021 ggaaagatta cttcgtgcaa tatttggaga aaaggctaga gaagttagag atacttcact
     4081 taaagtgcct catggtgaat ctggtataat agttgatgta aaagtattta ctagagaaaa
     4141 tggagatgat ttatctccag gagtaaatga attagtaaga tgctatatag ctaaaaagag
     4201 aaaaataaaa gttggagata aaatggctgg tcgtcatggt aataaagggg ttatctcaag
     4261 agtattacct gaagaagata tgccattcat ggaaaatgga acaccactag acataatact
     4321 taacccacaa ggtataccat cacgtatgaa cattggtcag gttttagaag ttcatttagg
     4381 acttgctgct aagacattag gatggtatgt agcaacatca gtattcgatg gtgctaatga
     4441 gtatgacata atggatgctc ttgaagaagc tgggtatcct agagatggta aattaacttt
     4501 atatgatggt agaacaggtg agtcttttga caacagaata actgttggat atatgtacta
     4561 cttgaaacta catcacttag tagatgaaaa actacatgca agaagtactg gaccatactc
     4621 attagttact caacagccac taggtggtaa agcacaattt ggtggacaaa gatttggaga
     4681 gatggaggtt tgggctctgg aagcatatgg agctgctcac atacttcaag aaatacttac
     4741 tgttaagtct gatgacgttg taggtcgtgt tagaacatat gaagctatag ttaaaggtga
     4801 aaatatacct gaaccaggaa tcccagaatc atttaaggtt cttataaaag aacttcaaag
     4861 tttatgctta gatgtaaaag tattgacaga tgaagaccaa gaaatagaag taagagaatc
     4921 tgtggatgaa gatgatacaa taggtgaatt tgagctagat gttgtaaatc atatgggaga
     4981 agttgaagaa agtaatataa tagaagaaat tgaagatgat tttgcagaaa atgcagaaga
     5041 tgaggatata gagaatttag aagaattcac tgaagatgat ttatttgaag aagaaataga
     5101 ttttgatagt gatgactttg atatgtaaat ttaattaatg taaatctcaa atagaaggga
     5161 gagaactcct tgtttgaatt aaacaatttc gagtcgataa aaatagcatt ggcttctcca
     5221 gaaaaaataa gacaatggtc taggggagaa gttaaaaagc cagaaactat aaattaccgt
     5281 actttaaaac cagaaaaaga tggtcttttc tgtgaaagaa tatttggacc acaaaaagac
     5341 tgggagtgcc actgtggtaa atatagaaga gttagatata aaggtgtagt ttgtgataga
     5401 tgtggagtag aagtaactaa atcaaaagta agaagagaga gaatgggaca tatagagcta
     5461 gcggctccta tgtctcacat ctggtacttc aaaggtatac caagtagaat gggactttta
     5521 cttgatatgt caccaagatc gttagaaaaa atattatact ttgcctcata tgtggtagtt
     5581 gatccaggag agactggatt aaatgaaaaa caattgctta cagaaaaaga atacagaact
     5641 gctcttgaaa agtatggata tacttttact gtaggaatgg gtgctgaagc tgtaaagaca
     5701 ttactacaaa atatagattt agagcaacaa agtaaagacc taagagcgga gttaaaagat
     5761 agtacagggc aaaagaaagt tagaacaata agaagattag aagttgtaga ggcatttaaa
     5821 aagtctggaa ataaaccaga atggatgatt ttagatgcaa taccagtaat accaccagat
     5881 ttaagaccaa tggtacaact tgatggtgga agatttgcga cttcagacct aaatgattta
     5941 tatagaagag ttataaatag aaacaataga cttaaaagat tattagagct tggagctcca
     6001 gatataattg taagaaatga aaaaagaatg cttcaagaag ctgttgatgc attgatagat
     6061 aatggtagaa gaggtagacc tgtaacagga cctggaaata gaccacttaa atctttatca
     6121 gatatgttaa aaggtaagca aggtcgtttc cgtcaaaact tacttggtaa gcgtgttgac
     6181 tactcaggac gttctgttat agttgttgga ccagaactta aattttatca atgtggactt
     6241 ccaaagaaga tggcattaga attattcaag ccatttgtta tggataaatt agttaaagaa
     6301 ggttatgcac ataatataaa aagtgcgaaa tctatagtag aaaaagttaa gccagaagtt
     6361 tgggatgttt tagaagatgt tataaaaagt catccagttc ttcttaaccg tgcaccgact
     6421 ctacatagat taggtataca agcatttgaa ccaatcttag ttgaaggtaa ggctataaaa
     6481 ctacatcctc ttgtatgtac agcttataat gcagactttg atggtgacca aatggcagtt
     6541 catgtacctt tatcagtaga agcacaagca gaagcaagat tcttaatgct ttctgtaaat
     6601 aatatacttg ctcctaaaga tggttcacct ataactactc catctcagga tatggtttta
     6661 ggttgctatt atctaacaat agaagcccaa gatggagcta aaggaacagg tatggtattt
     6721 aaagacttta atgaactatt acttgcttat tacaataaat cagttcatct acatgcatta
     6781 gtgaaactta aggtaacact tgaagatgga agaagttcat tggttgaaag tactgttggt
     6841 agatttatat ttaatgaaaa tatacctcaa gacttaggtt ttgtagatag aaaagaaaat
     6901 ccatttgcac ttgaagttga tttcttagca gataaaaaat ctcttggtaa aataatagat
     6961 aaatgcttta gaaaacacgg aaatacagaa acagctgaat tactagatta tataaaagct
     7021 ctaggattta aatattctac attaggtggc ataacagttg ctgttgatga tatgagtgtc
     7081 ccagaagaaa agaaagtatt tatagctgag gcagaagcta aagttgataa gtatgaaaaa
     7141 gcatacagaa gaggtctaat ctctgatgaa gaaagatatg aaaaagttat agagacatgg
     7201 acagaaacaa ctgataaagt tactgatgct cttatgggtg gactagatag attaaataat
     7261 atatatataa tggcacattc aggagccaga ggttctaaaa accaaattag acagctggca
     7321 ggtatgcgtg gtcttatggc aaatgcatct ggtaaaacag ttgagatacc agttaaatct
     7381 aatttccgtg aaggtttatc agtactagaa tactttacat cttcacatgg tgccagaaaa
     7441 ggtcttgccg atacagctat acgtacagct gaatctggat atttaacaag aagacttgtt
     7501 gatgtaagtc aagatgttat tgtaagggaa atagattgtg gaacagaaga tactacagag
     7561 atttatgcta taaaagaagg aaatgaagtt atagaagaga tatatgatag aattgtagga
     7621 agatatacta tagaccctat attaaatcct gaaactggag aagttatagt tgaggctgat
     7681 tcaatgatac aagaagatga agcagaaact atagtagctt taggaattga aaaaattagg
     7741 ataagaacag ttcttaactg taaaactaat catggagttt gttctaagtg ttatggtaga
     7801 aacttggcaa caggaaaaga agttaatata ggtgaagcag ttggtataat agcagctcaa
     7861 tctatcggtg agccgggtac tcagcttaca atgcgtacat tccatactgg aggagttgca
     7921 ggagctgata taactcaagg tctaccaagg gttgaagaat tatttgaagc tagaaaacca
     7981 aaaggattag ctgtaataac tgaagtatct ggtagagttg aaattgatga aacaggaaag
     8041 agaaaagaag taaatgtaat accagaagaa ggcgagactc aaacatatgt aattccatat
     8101 ggttctagat taaaagttaa gcaaggtcaa atgctagagg ctggagaccc tctaacacaa
     8161 ggatttataa atcctcatga tatagtaaga gtaaatggtg ttaagggagt tcaagaatat
     8221 atagttaaag aagttcaaag agtgtataga cttcaagggg ttgatgttaa tgataagcat
     8281 atagaagtta tagtaagaca aatgctatct aaagtaaaag ttgaagaccc aggagataca
     8341 gatttattgc caggtggata tgaagatgta ttaacattca atgaatgtaa taaagatgct
     8401 atagataaag gtctaagacc agcagttgct aaaagagttt tacttggtat aactaaagca
     8461 tctcttgcaa ctgattcatt cttatcagca gcttctttcc aagaaacaac aagagtatta
     8521 acagaggcag caataaaagg taaagaagac cacttaatag gacttaaaga aaatgttata
     8581 ttaggtaagt taataccagc aggaacagga atgaagaaat atagaaatat agctgttgaa
     8641 aaaattgaag attaatagca tatagagtaa agaaatcttg acaaccatta tttgtggtgt
     8701 taaaatagta aagtgtgttg tttaaaagta cactaagggc ttgaacaagt tcttagtgac
     8761 ttttagatgg caataaatga gaactaaaaa gcatcaagct agaaaagtgc taatatcaca
     8821 tatttatgtg ttatagacat taaattgtaa gtaacgaatg ttacaatcag tcagtatgaa
     8881 tattcatatt gatgtagttg gcggtcactg ataagatcga aaatttggaa ggaggtgcag
     8941 atgatgccaa caattaacca attagttcgt aaaagtagaa aagcgctaga gaagaaatct
     9001 actgctccag cattacaaaa aggttacaac tctttaaata agaaagtaac tgacgctagt
     9061 gccccacaaa aaagaggggt ttgtacttca gtaaaaacag ttactcctaa gaagcctaac
     9121 tcagctttaa gaaaagttgc cagagttaga ttaactaatg gtatagaagt ttctgcttat
     9181 ataccaggag aaggacacaa cttacaagag catagtgttg ttcttataag aggaggaaga
     9241 gtaaaagacc ttccaggggt tagataccat atactaagag gtactctaga tacagcaggt
     9301 gttgataaga gaagacaatc aagatctaaa tacggtgcga agagacctaa agaagctaaa
     9361 aagtaaaaac aattaatgat tgattatagt tcggtgcttt aaatatatat atatttacgg
     9421 catcacggca ttaaatgatt tatgtcgagt acctatgatt taagttaaat aattaaggag
     9481 ggaagcgaaa tgccaagaaa aggtaatatt ccaaaaagag aagttttacc agatcctatg
     9541 tatgggagta aggtagttac taaattaata aataatctaa tggtagacgg aaaaaaagga
     9601 aagtctcaaa gaatagttta tgatgctttc gcaattgttg ctgaaaagac aggagaagaa
     9661 gctttagaag tatttaataa agcaatggat aatataatgc ctgttttaga agtaaaagca
     9721 agaagagttg gtggggcaaa ctaccaagtg ccaatcgaag ttagacctga aagaagacaa
     9781 acattaggtt taagatggct agtaaaatat actagagctc gtggagaaaa aggaatggtt
     9841 gaaaagctag ctaaagagat aatggacgca gctaacaaca ctggagcttc agttaagaaa
     9901 aaagaagata ctcataaaat ggcagaagct aataaagcat ttgctcatta cagatggtag
     9961 gatatataga tatcttataa aattgaggat atcataagga ggataaccat ggctagaaag
    10021 tttcctttgg aaagaactag gaatatagga atcatggctc atatagatgc aggaaaaact
    10081 actactacag aaagaatcct gttttatact gggcaaactc ataaaatagg agaaactcat
    10141 gaaggagctt ctcaaatgga ctggatggag caagaaaagg aaagaggtat aacaataact
    10201 tctgccgcta ctactgcttc atggaaagac catagaataa acataataga tactcctgga
    10261 cacgtggact tcacagttga ggttgaaaga tctctaagag ttcttgacgg ttcagttgct
    10321 gtattctgtg caaaaggtgg ggttgagcct caatcagaaa atgtatggag acaagctgaa
    10381 acttatggag tacctagaat agcttttgta aataaaatgg atatattagg tgcagatttc
    10441 tacaatgttg taagcatgat gaaatctaga ttaaattcta atgcagttcc tatgcaatta
    10501 ccaataggta aagaagattc atttataggt ataatagact tattaaaaat ggatgctgtt
    10561 atatacaaag acgacctagg ggttgaaatg gaagaaacag atataccaga agatatgaaa
    10621 gaattagctg ctgagtggag agaaaaatta gttgaatcag tagctgaaac tgatgaagaa
    10681 ttaatgatga aataccttga aggtgaagaa cttactatag atgaattaaa agttgctata
    10741 agaaaagcta ctatagcttg tgaaatgaac ccagttttct gtggtacagc ttatagaaac
    10801 aaaggtgttc agttagtaat agatgcagtt ttagattact taccagcgcc aactgatata
    10861 ccagctataa aaggtatatt agcagatgga gaagaagctg aaagacattc ttctgatgaa
    10921 gagccattct ctgcattagc attcaaaata atgactgacc cattcgttgg aaaactagcg
    10981 ttcttcagag tttattctgg aacattagaa tcaggttctt atgttttaaa tgcaactaag
    11041 aataagagag aaagaattgg tcgtatatta caaatgcatg ctaatacaag agaagaaata
    11101 actaaagtgt atgctggaga tatagcagca gcagtaggat taaaagatac tactactgga
    11161 gacacattat gtgaccctgc taatccaata atacttgaat ctatggaatt ccctgagcca
    11221 gttatatctg ttgctataga accaagttct aaagcagctc aagaaaaaat gggtatagct
    11281 cttcaaaaat tagctgaaga agacccaact ttcacagtta agactgacca agaaacagga
    11341 caaactataa tatctggtat gggtgaatta cacttagaga taatagtaga tagattatta
    11401 agagaattta aagtagaagc taaagttggt gcaccacaag ttgcatatag agagactata
    11461 actcaaccag ttgatgttga atacaagtac tctaagcaat caggtggtag aggacaatac
    11521 ggtcacgtta agattagagt tgctcctcaa gagccaggtg aaggatacaa attcacaaac
    11581 aaaactgttg gtggatcagt accaaaagaa tatgttggtc cagttgacat gggtatacaa
    11641 ggagctatgc aatctggtat agttgctgga tatccagttg tagacgttgc tgttgagtta
    11701 tatgatggtt cttaccatga ggttgactct tctgagatgg cgtttaagat ggctggttca
    11761 atggcattca aagatgctat gaagaaaggt aatgcagttc ttttagagcc ttacttcaaa
    11821 gtagaagttg ttactccaga agattacatg ggagatgtta tgggtgactt aaactcaaga
    11881 agaggattaa tccaaggtat ggaagctaga agtggtgcac aagttataaa tgcattcgtt
    11941 ccactttcag aaatgtttgg atactcaaca gacctaagat cttcaactca aggtcgtgca
    12001 acttacacaa tgatattcga tcactatgag caagttccag cttcagttgc taagaaaata
    12061 gctgaaggaa aataatttaa aaataattaa taatataaat ataaaaaata atcagctaga
    12121 tgagagatat ctctcatcta gcacaagat
//
LOCUS       DAMJVI010000104        11997 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.109, whole genome shotgun sequence.
ACCESSION   DAMJVI010000104 DAMJVI010000000
VERSION     DAMJVI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.109"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            161..544
                     /locus_tag="PC545_002989"
     CDS             161..544
                     /locus_tag="PC545_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDF2457741.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLEDKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            546..896
                     /locus_tag="PC545_002990"
     CDS             546..896
                     /locus_tag="PC545_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457742.1"
                     /translation="MDNTITINISIIYVLLAILACIALVYLIIVLVKLHKILNNVGTM
                     LNENTQNINTTLNNLPNVVETFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFVDKK"
     gene            complement(997..1455)
                     /locus_tag="PC545_002991"
     CDS             complement(997..1455)
                     /locus_tag="PC545_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457743.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSSGRADFLYSSRLQKLLNEVYINQSRASYISGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(1500..2288)
                     /locus_tag="PC545_002992"
     CDS             complement(1500..2288)
                     /locus_tag="PC545_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2457744.1"
                     /translation="MKVVIIPGHTLIGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNATTSTPVGTETIYKTNNGKTY
                     AERVNTRLATVFKDRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGENKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALQEIDKL"
     gene            complement(2427..2864)
                     /locus_tag="PC545_002993"
     CDS             complement(2427..2864)
                     /locus_tag="PC545_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HDF2457745.1"
                     /translation="MNKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKNCSIYVTHF
                     PCLNCTKAIIQAGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            3686..4195
                     /locus_tag="PC545_002994"
     CDS             3686..4195
                     /locus_tag="PC545_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457746.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVESEVDNLKVISEKLEIINLQLAQRKNMRRKMFHWLF
                     ILLCAVILIVFVVLGRLNSPYLDWNYTDPETAVFGVAFHAFEWLFVRLVPIVLIGAII
                     GIFLTRKKI"
     gene            4222..4371
                     /locus_tag="PC545_002995"
                     /pseudo
     CDS             4222..4371
                     /locus_tag="PC545_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005342994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            4609..5040
                     /locus_tag="PC545_002996"
                     /pseudo
     CDS             4609..5040
                     /locus_tag="PC545_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5227..5420)
                     /locus_tag="PC545_002997"
                     /pseudo
     CDS             complement(5227..5420)
                     /locus_tag="PC545_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            5522..6028
                     /locus_tag="PC545_002998"
     CDS             5522..6028
                     /locus_tag="PC545_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2457747.1"
                     /translation="MKNEKKITVALLIVYLLILSWIILFKLQFSFSALDHIRQINLIP
                     FGGSVIANGKIDFDEIINNAIVFIPVGAYFSLLFKNKSVLKAIGSVLGISFVYEIIQF
                     IFAIGASDITDLISNTLGGIIGIALVYVLSIVLKDKTHKILNRIAIVCTVLVVAFLFM
                     LLAVNNIF"
     gene            6033..6309
                     /locus_tag="PC545_002999"
                     /pseudo
     CDS             6033..6309
                     /locus_tag="PC545_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
     gene            6578..7150
                     /locus_tag="PC545_003000"
                     /pseudo
     CDS             6578..7150
                     /locus_tag="PC545_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            7253..8068
                     /locus_tag="PC545_003001"
     CDS             7253..8068
                     /locus_tag="PC545_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDF2457748.1"
                     /translation="MFKIGEFSKLTQVSTRMLRYYDETGLLKPAKIDSLTGYRMYSAE
                     QISTLNRIIYLRDSGFNVAEIALALNFSNNAIIEQLDRKSIEIMDNIQKEQEKLKKIE
                     IAKKEVLHGKSELYFNITIKEIPSYCVLSLRKEIPNYYYEGELWKELSAFAKENKINI
                     SDETFSIYHNEEYKEKDVDVELCAIVNKIGKATGDFKFRFTEPVSMMACTMVYGDFSN
                     ISYAYRSFAKWLQENSQYKMKGSSRQIVHRGPWNEKNPENYLTEIQIPLENLL"
     gene            8149..8625
                     /locus_tag="PC545_003002"
     CDS             8149..8625
                     /locus_tag="PC545_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HDF2457749.1"
                     /translation="MTTYKVNSIGTICNNEKGAFIQLEAPYILALRALDGFSHINVLW
                     WFSDFDNTNFRSVLETEQPYKNAPEKMGIFATRSPVRPNPIALSAVEIIDIDYMSGLI
                     QIAYIDANDKTPILDIKPYTPSLDRVENPQVPDWCSHWPHSLEESASFAWESEFNF"
     gene            8639..9202
                     /locus_tag="PC545_003003"
     CDS             8639..9202
                     /locus_tag="PC545_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457750.1"
                     /translation="MSLQDYIFEKPVIETEHLILRTMNKNDIDDLKEWISDISLYEYW
                     GKRPSKSEKNPELLFDKSKKSTKSFHWGIVHKQDSKVIGEIWIYLIENNRMAKVAFRL
                     SKHYQGNGLMSEVLKNVIEFCFSKTELQKIWTDVHILNHASYKTLEKVGFQREGHIRG
                     GKMVNTYCDYYLYGILKSDYITRWCYG"
     gene            9457..10368
                     /locus_tag="PC545_003004"
     CDS             9457..10368
                     /locus_tag="PC545_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2457751.1"
                     /translation="MYAYENIEMSLNYIEQHLSEKIETEKLAEIACLSTFYYQRLFKR
                     LVKKSVQEYIKLRRLAMVIAELNNSEERILDIALKYGFSDHANFTRAFKEVYHITPDE
                     YRKNVPMLNTFDKPELSSRYIMIDENVPLIVGNIVLEIQRKTLRTKETYFGFEKTVNI
                     SEQIPAGESTGIDVPGQLWREFHAKKLQTENAIFNNDIELGVSHLASPDKGTFLYFAG
                     GMVSSTDVCPKNMVQHTLPAGEYIVCKIEAESFEELVTTALNQANKYLFETWLPNHNL
                     VTKPFMAEKYYKGNAEINYMEIWIIPV"
     gene            10446..10880
                     /locus_tag="PC545_003005"
     CDS             10446..10880
                     /locus_tag="PC545_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2457752.1"
                     /translation="MINIKDINYVPNISEISVYIENSAFDELYQHMNNEYKAVCKIEY
                     SKDVWARGWNVKFKKSGKSLCVVYPKKNYFTVLVVVGNKEKEDVENLLPRLSEYVQEI
                     YRNTREGNGQRWLMIDLKSKNNVYQDVLRLIEIRYAIKCVKP"
     gene            11348..11997
                     /locus_tag="PC545_003006"
                     /pseudo
     CDS             11348..11997
                     /locus_tag="PC545_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         4271 a         1744 c         2053 g         3929 t
ORIGIN      
        1 aaaatatata gaaaatcctt ataaaaattt aaattgtaat gaataggaag taattaaaaa
       61 gaatttttat tgatttttaa aaatttagtt agaaactata tcattatggt aaaaaatgta
      121 ttataatgtt tagaaatatt ataataagga gcgattaaac atgaatctat cgaagaaaat
      181 agaagataaa agaaaagcta aacagagagc tgaaaaagtt aagaaagcta aaattgctac
      241 agctggtgtg gtattaggag ctgttactgg tgcagtgagt ggagtacttt tggctcctaa
      301 atctggaaaa gaaacaagag aggatataaa agatgcttca caacaaattg ctgaaaagat
      361 aaatatgaaa actgtagatg taaaaggcaa agtatctgag aagctagagg ataaaaaagg
      421 aaactttata gaatcaaaga aaaaaataaa aaaatatata gataacaaaa agagtgttga
      481 aaattctagt gaggaagata aaataataga tatagttgaa ccagttagtg ttactgagga
      541 ataatatgga caatacaata acgattaaca taagtataat atatgtatta ttagctatac
      601 ttgcttgtat tgctttagtt tatttaatta ttgtacttgt taaactacat aagattttaa
      661 ataatgttgg aactatgtta aatgaaaata ctcaaaatat aaatacaact ttaaataatc
      721 ttcctaatgt tgtagaaact tttggagaag taggagaaaa tatgaaagat gtaacagaag
      781 tagttactga agttacagca gattttcttg ttactaaaga cagtgtcaaa tcaaatatgg
      841 atgttatagt tgatatattg actataatta aaaacatttt tgtagataaa aaataataag
      901 ttaatataaa ataagcctat aagatataat ttaaacgacc ttaaaatagc atttataagg
      961 tcgtttttta ttagcttgat ttgacactat gtgtttttaa taattggtaa tattcctctt
     1021 tatcatttga acatatatat ctatgcttgt tgtttcaatt acatccataa gaacatcttt
     1081 acattcaagc tcaaaatctt ccattccttc taaatattca tcatcttcat caaaaaagat
     1141 ataagaatta tatagccatt cttcaaattt ttcttcaagt atatcccaat acgaactcca
     1201 aaataatcca caacctgata tataagatgc tctactttga tttatataaa cttcatttag
     1261 aagcttttgt aatctactgc tataaagaaa atctgctctt ccactgcttg atattaattc
     1321 atttaaatct tctattgaca ttaattcaac tttaccactt ttatcataag gagtataatt
     1381 ttcatcaacc catttatgtg gtttgttttc tctgcatact gttgctatca actctacaac
     1441 ttttgttctt atcatttttt tatcctaact tatttatttt ttataaaata atcatgattt
     1501 tataatttat ctatttcttg taatgctaat ttaacagttt catatctatc agcaccattt
     1561 attactacat caccttttac aagtttatta gcttctccac ctacaacata aaccttttta
     1621 cctaaacctt gcttataact agacgcttca attattaaag aatctttcaa ttgccaatgt
     1681 aaaatctgag cagccacttt atcagcatca cctgtataaa cgattacatt ttcatacact
     1741 ttattttccc ctcccccttg agaatttgat atagatttgt ttagtatacc ttctgctatc
     1801 aatttagcaa ctttatcttt attattaaca taataatctg tatctgcttt actatctaca
     1861 aaacatactt ctattaatat tgctggtgca atagtatgat taagccaata aagacctcta
     1921 acatcagact ttgcacccct atctttgaat actgtagcta acctagtatt cactctttct
     1981 gcataggttt tgccattatt agtcttatat attgtttctg tccctactgg agttgaagtt
     2041 gtagcatttg aattaaaatg aatctgaaca gccaaatcag tttcctgttt atttgctata
     2101 gcacattggt ctgctaaatg attacttgat tcatcaactc ttcctgtata aacagtagct
     2161 ccacctattt taagccattt aacaattaag tcattcagta ttcttgtttc tttgctttcg
     2221 tttatatacc caacagctcc tgtacctttt ccaattaaag tgtgccctgg tattattact
     2281 actttcaaac taacatctcc tttacaatta aatttaactt ctatttaata tatgcataac
     2341 ctagaatttt gtttcttttt atatcttata cttttattat aacccatttt gtaaaaatat
     2401 agaatattaa gtaaataatg ttaaaattat aattttgtat ataagacatt acttgattgc
     2461 aataatttta tagcatattc atctattcta tatccagttc tataatatat atgtttaatt
     2521 ccagcttgaa ttatagcctt agtacaattt aagcatggaa agtgagttac ataaatagag
     2581 caatttttaa ctgaaatacc ttcttttgca caataataaa gggcattttg ttcagcgtga
     2641 atggttctaa tacaatgtcc atctctcatt tcatgaccaa cttcactaca atgcttatct
     2701 ccagaaatac taccattata tccagtagac actattctgt tttctgaatt gacaattata
     2761 gctcctacat aagctctatc acaagttcct ctttcagcta ctgtttcgca cagtctcata
     2821 aaatattctt gccatgagca tctattttca agttttttat tcaaatcata tacctccaaa
     2881 atttatatct aatatataag atttatacct taaaaataat attaaaaaca aatttatatg
     2941 ataaataaaa tttattaata ctgtttttag tctaaattat ttcattttaa attaaagtta
     3001 caaggaatct aagattatat atatctttct tcgtaatatg aatgttacaa tataaaaaac
     3061 tgtctcaata aaatagagac agttttacag tcaaagttta taatacaagt tcatattttt
     3121 ctttaaattc ttttacagct tcgggccatg ccttttgttc tgtaatacct ttttctttag
     3181 ctattttaat tgcaatctct cttatcattg cagcgccttc taatattccc atttttaatc
     3241 tcccttcaaa tatctttttg tatagaatat aaatagtgct aagatttatt acatgctgca
     3301 tatacaatat ctatacaaca cataacttgt aaattccttt tattttcata cgtctttaac
     3361 cgacatataa ttatattcaa taagttttaa aattatgaac tcaattataa ttatttttat
     3421 ttttttagaa tctaaacact aaaaattgta ggtaaatttg tcttatgcct ttataaatat
     3481 ttctacagtt ttaattactg gacatatcta tttttattat aaaaagcgat taaaaatgga
     3541 gaatatcctc ataaaaacta tatataagca aaacggttaa aggaaaaaga agaaatttac
     3601 ttgaatgtta aaatgcgttg cttgaagcaa tgggcagttt tgcctataat atcggtaaca
     3661 actgaaagaa aggtggttag ccctaatgtt aagcgaaaac attaaaacaa tcaggaagtc
     3721 aaaaggactt tcacaacaag aacttgctat aaaactgaat gtagtgcgac aaacaatatc
     3781 taaatgggaa caaggactgt cggttcccga ttctgatatg ttgatttcca tatcagaggt
     3841 gcttgaaacg cccgtaagca ctttacttgg agaaactatt gttgagtcgg aagttgataa
     3901 cttaaaagtg atttctgaaa aattagagat tataaacttg caacttgcac aaagaaaaaa
     3961 catgagaaga aaaatgtttc attggttatt tattttatta tgtgcagtca tattaatagt
     4021 ttttgtggtc ttagggagac taaatagtcc ttacttggat tggaattata ccgaccctga
     4081 aaccgctgtt tttggagtag cttttcatgc ttttgagtgg ctatttgtca gattagtacc
     4141 gattgtcctt ataggagcaa ttattggaat ctttttgaca cgtaagaaaa tatgaagtaa
     4201 ctctgctttt tataatacag caagcgttcc ttaaaagcgt cattctacat ggcaatcgag
     4261 gacgactatg taaggaagaa cctttttgat ttccaactaa gcgaagttat tgaggacgac
     4321 agtaggccca aaaccgcatt aagtaaagta caggaagcaa gactgctgga cttatactct
     4381 catgcgtcag atgcaggaaa tgcgaggtct ggtagcatga tttacaacga tttgtacaac
     4441 gactacgagt agaaatatat ggagataagt attatctacg aaatcagaaa agaaatgact
     4501 tgataattta ctcatgttat aattattgat gtgaaattaa taaatcgaaa ttcctttttt
     4561 ggatttaaca atactgagta aggaaaaagt gaattacttt tgcaaggagg tgttatttgg
     4621 atggtgctag caggtatttt gattgtagta caaggctttt tgactggtcc tttagcaaaa
     4681 aaaattggtg agaagcgact aatactgata ggtatgtttt gcaaagcctt agtagcgttt
     4741 gccatggtat tgacaactgg gtttgttagt gtgttggtgg tttttggctt atttgcggta
     4801 tcttctgcta ttacagtacc cacattaaac gcaacccttt caaaaactga caatcagcat
     4861 aaaggaacac tgatgggatt tgccagtacc gccggaaatc ttagcaaagt tattgctcct
     4921 ttattaactg ggtatctgta tgaaagcaat attgaattac catatataac cacgggaggg
     4981 atagccttaa tcggagtggt tatctgctat atctggataa aaaatcaaaa gaacaaatga
     5041 tggaagagga cgtttatatg gaggttatac acagtaacta atctggtata gaaattcaat
     5101 tgaggctttt ctgctttatt aaagtcttgc tactacttaa ccctataata tccttaattc
     5161 attatgacaa attccaattc aacagttttg atacacataa ggaggtaaca atactatgga
     5221 aaattatcaa tgaagtgcta attctttatc agtgaagtta tccataagtt tttctacctg
     5281 taatcgtctg gtgctttttg ccagatcatt ggtaggtaag aaaaattcat caacagacac
     5341 atgaagtaat gatacaaggt cacttgtatg cttggatgtt gccctttatt ttcaatatta
     5401 gttaagtaac gtgggtcaat atttgtaatt cgattttagg gaaatattta aagaaatatt
     5461 ttcgttttat ttctttaaat cttcaagaat attgatagtt ttttgaaagt aggtgtgtat
     5521 tatgaagaat gagaaaaaaa ttacagtcgc attgctcatt gtttatttat tgattttatc
     5581 atggattatt ttatttaagc tacaattttc attttctgcg ttagaccaca taagacaaat
     5641 aaacttaatt ccttttggtg gttcagttat tgcaaatgga aaaattgact tcgatgaaat
     5701 tataaacaat gccattgttt ttatccctgt aggtgcatat ttcagtttgc tttttaaaaa
     5761 taaatctgtt ctaaaagcaa taggttctgt tttaggaatt agctttgtat atgagataat
     5821 tcaatttata tttgctatcg gagcaagtga tattacagac ttaattagca acacattagg
     5881 gggtataatt ggaattgcat tagtatatgt attatctatt gttctaaagg ataaaacaca
     5941 taagatatta aatcgaatag caatagtatg tactgtatta gtagttgcct tcttatttat
     6001 gcttcttgct gtaaataata tattttaatg ccatgttaat tacatgttgt tttggaaaag
     6061 actttaatct gtcaaagttt gcttttgaaa ttgaacatga aattacacca atggaacaaa
     6121 tgaaagctat aaatttttca ttggagaaag atgactgtac aagtaaagta ttaatataca
     6181 atccagacaa atggaaatat gtagaatact atttctataa agatagacct attaaaaaaa
     6241 ctattggaaa aatttattca attttgcatt tgtcacaata aaatatgttt tgaaatattg
     6301 aatacttaaa tatgcaaaaa aacaaatgta actggctatg gggctatatc tactaaaaag
     6361 ctatgtgata ggttgggatt aaatttaaac atcacttcta gaaggcatga atacacaagg
     6421 ctgacaaact atacagttat gattaaacga taccaacggg attgaaaatt tcgtgaagaa
     6481 ctttgagaag tcctattaca catgagcaac agcaacgaag ctattgaagc aaggacgcag
     6541 gcaatctagc ggttatctga caaaggagtt gtaggacttg aatgtagata ctatcagaac
     6601 ggatataccg accagctctg cggttacaga tgtgattgtg tagaatatcg agcagtcggg
     6661 aacggacact ttatctgcta cctacgaagt agatcagcag ataaaagaga gagaacagac
     6721 aacagcggtc aaggcaacct ataccgtaaa agtccatgtg gacgctgacg gggatatggt
     6781 aatcgttcag aaccttactc ttgcaccagc aatcgaaaaa tcagactatg agccaaagat
     6841 atcagaaaca gacgcaagcg tggacgctga taccgttaat gacgctaccg catttctgga
     6901 aacattcttt gaggtgtacc cgacagccac agaaaaagaa cttgcctatt atgtatcgga
     6961 aaatgtgatt gaacctatcg gcagggacta cctttattct gaattggtaa acccagtctt
     7021 tacaaaggac ggggacaatg tgaaagtcaa ggtagctgtg aaattccttg ataatcagac
     7081 aaaggcaacg caggtgtcgc agtatgatct tgtactccaa aaggatagca actggaagat
     7141 tttaggataa catggaaatg ggcttgtatc ataattatag tgtgcaagcc cttttcatat
     7201 tgaagtatca ataatcaata attataatta ggtacatgaa tggagggata aaatgttcaa
     7261 aattggtgag ttttcaaaat tgacacaagt ttctactcgt atgttgcgtt attatgatga
     7321 aacgggttta ttaaaacctg ctaaaattga ttcattaact ggatatcgta tgtattcagc
     7381 agaacaaatc tcaactttaa atagaattat atatttacgt gatagtggct tcaatgtcgc
     7441 tgaaattgca ttagccctca atttcagtaa taatgccatt atagaacagc ttgatagaaa
     7501 gtctattgaa attatggata atatacaaaa agaacaagaa aaactaaaga aaattgagat
     7561 tgctaaaaaa gaagtgttac atggcaaaag tgaattgtat tttaacataa ccattaaaga
     7621 gataccgagt tattgcgttt tatctttaag aaaagaaata ccaaattatt attatgaggg
     7681 cgaactctgg aaagaactct ctgcttttgc taaagaaaat aaaataaata tttctgatga
     7741 aacattctca atatatcaca atgaagaata taaagaaaaa gatgttgatg tagaattatg
     7801 tgccattgta aataaaatag gaaaagctac aggagatttt aagtttcgtt ttaccgaacc
     7861 agtttctatg atggcttgta ctatggtata tggagatttt tctaatattt cttatgctta
     7921 tcgttcattt gcaaaatggc ttcaagaaaa cagtcaatat aagatgaaag gttcaagccg
     7981 tcaaatagtt catcgtggac cgtggaacga gaaaaatcca gagaactatc taacagaaat
     8041 ccaaattcct ttagaaaatc tcttgtaaaa aatgataatc tgccttgact ttaacatggt
     8101 gttaaggttt atcgtttatt tatcaagaag aaaacggagg aaaatataat gacaacttat
     8161 aaagtaaatt caataggtac tatctgcaac aatgaaaaag gtgctttcat tcaactagaa
     8221 gcaccatata ttctagcatt aagggcatta gacggattca gtcatataaa tgttttatgg
     8281 tggttcagtg attttgataa tacgaatttt agaagtgtat tggaaacaga acagccttat
     8341 aaaaatgctc cagagaaaat ggggattttt gcaacaaggt cgccagtacg cccaaatccg
     8401 attgctttat cagcagtaga aattatcgat attgattata tgagtgggct gatacaaatt
     8461 gcttatattg acgctaatga caaaacgcct atcttggata ttaaaccata cacgccgagt
     8521 ttggatagag tggaaaatcc gcaagttcca gattggtgta gtcattggcc tcacagctta
     8581 gaagaatctg cttcttttgc atgggaaagc gaattcaatt tttaaggagt gatgaaccat
     8641 gagtttgcag gactacattt ttgaaaaacc agtaattgaa acagaacatc ttattttaag
     8701 aacaatgaat aaaaatgaca tagatgattt gaaagaatgg attagtgata tttctttata
     8761 cgagtattgg gggaaacgtc cctcaaaaag cgaaaaaaat ccagaattac tctttgataa
     8821 gtcaaaaaaa tctactaaaa gttttcattg gggtattgtc cataaacaag atagcaaagt
     8881 gattggtgaa atatggatat atcttattga aaataataga atggcaaaag tggctttcag
     8941 actttctaag cattatcaag gtaatggact aatgagtgaa gtgttgaaaa acgtaattga
     9001 attttgtttt tcaaaaacag aattacaaaa aatatggaca gatgtgcata ttttaaatca
     9061 tgcttcttac aagacattgg agaaagtcgg ttttcaaagg gaaggacata ttcgaggggg
     9121 gaaaatggta aatacctatt gcgactacta cctatatgga attttaaaat ctgattatat
     9181 cactaggtgg tgctatggct aatattgaag cgacgattgg cgaagcaatg aacaatacag
     9241 ttccaaaggt ctatgatacg aaaagcatta aaagatatgt gatgaattta ttagatatgg
     9301 ctcattaaaa agtaccaaag aaataaaagt taccatatag acaaaattgg ttttttatat
     9361 cttttgattt cttataagat aatggcattg tgcgacaagc tatgcaaaaa attttcatgt
     9421 atactttgat tataatttat acaaggaggt actgatatgt acgcatatga aaatattgaa
     9481 atgtcattaa actatattga acaacattta agtgagaaaa tcgaaacaga gaaactggcg
     9541 gaaattgcct gtctttctac attttattac caacgcttgt ttaaaaggtt agttaagaaa
     9601 tcagttcaag agtatatcaa acttcgccgt ttagcaatgg taatagcaga attgaataat
     9661 tcagaggaaa gaatattaga catagcttta aaatatggat tttcagacca tgctaacttc
     9721 acaagagcat ttaaagaagt ttaccatatt actcccgatg aatacagaaa gaatgttcct
     9781 atgttaaata catttgacaa gccagaactt tcttcacgtt atatcatgat tgatgaaaat
     9841 gtcccattga ttgtaggaaa tattgttttg gaaattcaaa gaaaaacatt aagaacaaag
     9901 gaaacatatt ttggttttga aaaaacagta aatatttctg aacaaattcc tgctggagaa
     9961 agtacaggaa ttgatgttcc aggtcagcta tggcgcgaat tccatgcaaa gaaactgcaa
    10021 accgaaaatg ccatttttaa caatgatatt gaacttggtg tatctcactt agcaagtcca
    10081 gataaaggaa cattccttta ctttgcagga ggtatggttt catcaacaga tgtatgtcct
    10141 aaaaatatgg tacaacatac attaccagca ggtgagtata ttgtatgcaa aattgaggca
    10201 gaatcttttg aagaacttgt tactactgct ttaaatcaag caaataagta tctgtttgaa
    10261 acttggcttc caaatcataa tttggttaca aagccattca tggcagaaaa atattacaaa
    10321 ggaaatgcag agattaatta tatggaaata tggataattc ctgtgtaaac agtaaatgct
    10381 aaataggaat tatctgacgt aataaaaagg aaatagaaag cttataaagg agggcatagt
    10441 ccgttgtgat aaacataaaa gatataaatt atgtacctaa tattagtgaa ataagcgttt
    10501 atattgaaaa ctctgcattt gatgagttgt atcagcatat gaacaatgaa tataaagcag
    10561 tttgcaaaat cgaatacagt aaagatgtct gggcacgagg ttggaacgta aagtttaaaa
    10621 aatctgggaa atcattgtgt gttgtctatc cgaagaagaa ctactttact gtattggtgg
    10681 ttgtaggtaa taaagagaaa gaagatgttg aaaacttgtt acctaggctt tctgaatatg
    10741 tacaggaaat ttatcgtaac actagagagg gaaatggaca aaggtggctt atgatagact
    10801 taaaatctaa aaataatgta tatcaagatg tattaaggct tattgaaatt cgttatgcca
    10861 taaaatgtgt aaaaccgtaa taaaaatatt tttcacatac gtaatctcaa ggtattatcg
    10921 ttgagacaag taacctatat aattatggga aatgagattt ttagatactt aagaaagtac
    10981 tttagggggc ttgcgttatg gaaagattta agtacaattc cacacaatca aatacaatct
    11041 aataaaactt tactaaaaat tgaatgcaat acagctttaa aggctcgtac aaactatatg
    11101 ttttgtgcgg gcttttttca tttaatagga atttggattc gtcagagagt atattataaa
    11161 aataaaacga taagggtacc ttaaaaataa ggtacctcag gctgtagaca aagtcccgga
    11221 ctctcgtctg ggcttttctt atatttgagt tatccacaat tcaacttttt aagagttatc
    11281 ttattaagaa aaaagtgtat gttttccacc ctatagtttt acttctgtga tatttgcaga
    11341 tgccactcaa gtagaaagtg gtggtacggc agaagatgtc atgcagagtt cagaaagtct
    11401 gggactaccg cctaattcct tagacacgga aagctcaatc aagcaggggt gtaagtattt
    11461 tgcgtccctg ctttcttcct gcaaaaatca aggtatcgac gatttgaatg tagcgataca
    11521 atcttacaac tatggcgggg gttatgtggg ctatgtggca ggaaaaggga aaaagcacac
    11581 ttttaatctt gcagagagct tcgcccgtga gaaatcgggt ggaaagaaag taacctacgc
    11641 caacccgata gccgttgcaa agaacggggg ctggcggtat ggctatggaa atatgtttta
    11701 tgtggaatta gtcaatcagt atttaactgt tccgcaggtg tcgggggaac tggcacaaaa
    11761 ggtaatgaat gaagcgttga agtatcaagg gtggaaatat gtgtttgggg gaagcaatcc
    11821 caacacttcc tttgattgta gcggacttgt gcaatggtgc tatggaaaag ctggtatcta
    11881 cttaccgaga acggcacaaa cacagtatga cgcaacccag catatccctt tatcgcaggc
    11941 aaaagctggg gagttgttct ttttccattc aacctataac gctggttcgt atgtaac
//
LOCUS       DAMJVI010000105        11843 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.110, whole genome shotgun sequence.
ACCESSION   DAMJVI010000105 DAMJVI010000000
VERSION     DAMJVI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.110"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            779..1810
                     /locus_tag="PC545_003007"
     CDS             779..1810
                     /locus_tag="PC545_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDF2457753.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGFT
                     LDGDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLATRNHWDSCDFDECSRIVNFKLDLYDKENALFMKVADVVRDCL
                     DIEENNEKLFKGSLFNKEIGLVYDNCFNLLKKLEHRFDIKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
     gene            1943..3241
                     /locus_tag="PC545_003008"
     CDS             1943..3241
                     /locus_tag="PC545_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDF2457754.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVKEGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVDFDDINIKKSDINSQIYDCTVMKETYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLINTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKG
                     RKLTLLIGMLEDKDIDSVLEILLPHFNKVITTTPSNPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(3355..3600)
                     /locus_tag="PC545_003009"
     CDS             complement(3355..3600)
                     /locus_tag="PC545_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HDF2457755.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            3748..4614
                     /locus_tag="PC545_003010"
     CDS             3748..4614
                     /locus_tag="PC545_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDF2457756.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            complement(4618..5154)
                     /locus_tag="PC545_003011"
     CDS             complement(4618..5154)
                     /locus_tag="PC545_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HDF2457757.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(5345..5548)
                     /locus_tag="PC545_003012"
     CDS             complement(5345..5548)
                     /locus_tag="PC545_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HDF2457758.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            5839..6603
                     /locus_tag="PC545_003013"
     CDS             5839..6603
                     /locus_tag="PC545_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2457759.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKRTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESQPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            6992..8305
                     /gene="prdC"
                     /locus_tag="PC545_003014"
     CDS             6992..8305
                     /gene="prdC"
                     /locus_tag="PC545_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HDF2457760.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQEDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     regulatory      8709..8794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            9376..11625
                     /locus_tag="PC545_003015"
     CDS             9376..11625
                     /locus_tag="PC545_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDF2457761.1"
                     /translation="MKTKIKKSSIISLVIAFSMVFTAFTPIVSYADEVTSNDTILNGE
                     EQSNGQTSDVEKPSDGQVPDGEKPSDSQVPDVEKPSDGQVPDGEKPSDSQVPDGEKPS
                     DGQVPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQIPDGEKPSDGQMPDGEKTSDSQ
                     TPDVEKPSDGQMPDGEKPLDEQTPEEEKPLEEEIVIEEMSLKQDIDKILDMTLSQINK
                     IVYNFWEDEEDVKADEQSEINQVFTSEDSFISLWYDKKAKVKNSCLLKEIDGSDRPYH
                     DLRFDDITKTLTFDYVIKGLVGASSKDDKYVIEGEDGEQTAFCYNNHLRPPSSNGKSP
                     YLPAEDFNGQENQNEEAVKSILYAGSEFDGFGYKQQFNLGGEENELMTYSATQSAIWI
                     ILGQMDEQERLKQYQGSINLCDKLIERAKTEEERAEYQKKKEVAINIKAYLEALLKAG
                     REELKPNNTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSGVVKLKLPDGVTAYDG
                     DGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAVSYDYIFPKAIQYYKAVLDLGQKD
                     HSSSLPSSKQNLLSYTIEKKNGQEVNFNIGLPTDDDDVVNPPVPPIDDDVVNPPVPPT
                     DDTNGHKPKPSPPIDDTVINPPVPPMDDTIINPPVPPTEDAVLNPPVPPMEDAVLNPP
                     VPPTEDTVLNPPVPSVSDTVEKTPELSRDDTIIKSPKTGDETQLMSYVFISVIAICGL
                     AYQCKIKRN"
BASE COUNT         4777 a         1305 c         2024 g         3737 t
ORIGIN      
        1 tttaaaataa tgactattta taaaactcta tagatatttg tttcatttag ctatgcatac
       61 tttaattaat tatattaata tttaatggag tacaatataa atattaaagc gacatcttat
      121 agtttattga acctatagta atgaattaat aaatagttta attctatgaa aagatttcct
      181 tttatgcaat ttatttattg aaatatgata taatatataa aggtgtaagt attagttgtt
      241 aaaataatat ttatattagt ttttatgcat acttggtata agtttttcat atattgatta
      301 aaattgatgt tgatttatta tttttagtat tttttcacct tttgattcat actttgtaga
      361 atggtgtatt ttctaaaact gattataaga caagttcatt caaattagca tttttatatc
      421 atataaataa ttgttccccc taaataagat ttagatgtat agtattaaaa tcaaaaagta
      481 taaaataaaa ttgatgctta atcatagata aatattaata aaatatacaa ggggattgtc
      541 taataaaaag ataatctcct tttttacaaa taattctaaa ttgtgatata aatctaaaaa
      601 ttttatctta acatttaacc atttaatgat aataattgat ataataaata taaattaaat
      661 atatggtgta cttattttat gtttttagca agaaatatag catttaaaag ataaattgtg
      721 caacttaaat caaagtttaa gatgaactat taacatagat atattaaaaa cttttgtaat
      781 ggagaatata tgtgatatga ataataaagt taaggttgca gtattagata caggaataga
      841 caaagaacat gattacttaa aagataattt agttggagga attgcttttg aatgtatgca
      901 tgattatatc tttatatcag ataaatttga tgatgaagat ggtcatggaa ctgcttgtgc
      961 atcaataata aagaaagagt atgaagatgt agagttattt gtaataaaaa ttttgagaaa
     1021 agatggtatt acaaatataa aagttcttga agaagcatta agataccttt tagacactaa
     1081 cataagatta attaatttaa gtttatcagt cataggagta gaaagtgtta agggcttatt
     1141 tgaagtttgt tatgaattgt ttagaaaagg aaaaataata gtatgttcac tcgctaatga
     1201 ttttgactta agttatccag ctatgtttaa caatgttata ggtgtaagag gatttacttt
     1261 agatggagat gattcatttt ggtacaacaa aaaatatgat gttcaatgtg ttatggatag
     1321 caattcatat ataagttgtg atataaataa ttcctataga ttaccaccaa aatgtaatag
     1381 ttatttatca gcaaagctta ctggtaaaat agctaagata ttatcagaaa aaccaggcat
     1441 aacaatatct gatttaaatg ataagcttga atcattagca actagaaatc attgggatag
     1501 ttgtgatttt gatgagtgta gtagaattgt aaactttaaa ttggatttgt atgacaaaga
     1561 aaatgctctt tttatgaaag ttgctgacgt tgttagggat tgcttagata ttgaagagaa
     1621 taatgaaaaa ctctttaagg gtagcctttt taataaggag ataggcttgg tttatgataa
     1681 ttgttttaat ctgttgaaaa agctagagca tagatttgac ataaagttta attatatgga
     1741 catctcaaaa tatgacttag tttcaatata tacactgact gaattagtgg aaagatataa
     1801 aaacttataa ataaagattt gtagctaaat ttaatgtaga aggtattaaa ttatatgtat
     1861 tctttgtcgg tagattaaaa gtaaaaaata tactataatt agatagcata agagaaaatg
     1921 ccaatacagg gggagttaga taatgaaata tgaagaagca ttagagtaca tatcacaaac
     1981 taataaattc ggtataagat taggattaga aaatatcgga aagttattag aattattagg
     2041 aaatccacaa gaaactttga atataataca tgtagctgga actaatggaa aaggttctgt
     2101 gtgttctttt gtttctaata tactaagaga gtgtggatat aaagttggtt tatatacatc
     2161 tccatattta gaaactttta ctgagcgtat aagagtaaat ggacaaaata taccacagga
     2221 tgatgttgct agaataattg agttaataaa agaaaaaata gagattatgg taaaagaagg
     2281 ctatgcatat ccaacagaat ttgaagtggt tactgcaatg gcattttatt actattcaga
     2341 acaaaaggta gactttgtgg cattagaagt tggtttaggt ggcagatatg atgctacaaa
     2401 tataataaca aaatcacttg taagtgtgat agtatctata agtttggacc atacaggtat
     2461 acttggagat acaatagaaa aaatagcgta cgaaaaggct ggaataataa aggaaaatgg
     2521 agtagtacta gtatatgacc agactgatga agcaaaggat gttataaagt cagtttgtaa
     2581 agagaaaaaa gctaaatata tagaagttga ttttgatgat ataaacataa aaaaatcaga
     2641 tataaattct caaatatacg attgtactgt aatgaaagaa acttatagag accttgaaat
     2701 aaaattgata ggcgaacatc aaataaacaa ttctatcttg gcaattagtg taataaaata
     2761 tctaaaagac ataaataaat tagcaaatat aaatgaagaa tctataagaa aaggacttat
     2821 aaatactaaa tggccaggaa gaatagaaaa gataaaagaa aatccaattt ttataataga
     2881 tggtgcacat aatgaagatg gagcaaaatc tttggcaaaa gcacttgata agaattttaa
     2941 aggtagaaaa ttaaccttat taataggtat gttagaagat aaagatattg atagtgtttt
     3001 agagatattg ttgccacatt ttaataaggt aataacaaca acaccaagta acccaagagc
     3061 cataaattca gatatattaa gagaaaaagt attaaaatat gtagatgatg taacatcaaa
     3121 gcatgagata gaggatgcag taaattatac attagaaaca tcaagtgagg atgatattat
     3181 aataagtgca ggttcattat atatgattgg tacagtgaga actttagtaa agaaattata
     3241 attttataaa ataagtatac aaataaaaat ataaaaaaga gttgtctaaa aaatggacaa
     3301 ctctttttat ataaaataag tttttaataa ttttactgat gtttatataa gtaattatat
     3361 agattgacgt atagctttag ataattggtc tgggcaagaa gtaccttttc caccacagtc
     3421 aataccattt aatttatcag caacttcaag agcattttgg ccaattatta agcttctaag
     3481 tcctgataaa ttaccattac atcctcctat aaattcagca tcaacaatca cattattttc
     3541 atcaacttca aaaatcattt ctctacaaca tacaccactt ggattaaatg ttactttcat
     3601 taaaaagacc tccattaaat attatttctt tttttaacat taataataat accaaatata
     3661 tgcgtaataa tgcaactata aagcataata caagctaatg aaataatata ttattgtagt
     3721 atatgtattg tagagaggag gggttatatg aaaatctata tttctaacta cttatcaaag
     3781 agtattagca tagttgatta ttctacactt gaactagaaa aagaaatagt attagaagat
     3841 aatatatatc cacaccattt ttgcatagaa aaagaaaaaa atttaatata catacctagt
     3901 tcaagtaatg ggatactcta tgtcttagat ttaagtaatg ataaaataat tgatactgtc
     3961 tctataggag gaagtcttag tcaagttgta cttagcgata atgaattgtt tgtggcaaat
     4021 gaagattcta atagtatata tgtactagat aaaaacacat taaatccaat aggaattatt
     4081 ggagttgata atatgccaca tggatttgac tttgatagag aaagcaaaaa gttatatgta
     4141 ccgtgtataa actcgatagt atgtatagat acaattaata aaaatataca aaagaaaata
     4201 aatatggact ttagagcttg gcatatagaa cttgataaac aaaagaaaga aatgtatata
     4261 tcaacacttg atggaaagct tataataata gatgaagtaa gcatggatat aaaaaaggta
     4321 ttatatgaat ttttattacc agtagaaata agatttaatt atagtggcaa aaaagtttat
     4381 gttgctgatt tgggttataa taatgtaaga attttagatt atacaacgag tagatacatt
     4441 ggaaatattg aaataaatgg gattccccaa ggattagaaa tatctaaaga tgaaaagtta
     4501 ctatttgtat cagatacaca agaaaattca gtaaaagttt atgaaactgc aaataacaaa
     4561 ttaataaaag aaatcaaggt aggaaaagag cctacaacta taatttgttt gtaggcttta
     4621 atgtatgtct cttataagta aatcgataac ttcggcatac atataagatg cattattttt
     4681 ccagttattg caaataagtt ttgcttgttt ctctgatgaa acatttagat ttaaatctat
     4741 taagttagac tgattttcta tgactctaag gttgacaata aattcattat cactttgttt
     4801 tacaaaacta gactttactt gagtatctgc tagtagattt tctttgttag aatctatata
     4861 cttgtctatt ttttcagtaa cactagtagg tattcgatta aagaagtatt ctagactttc
     4921 tacgcctctt tgagtaagag cataatattc tctgtcagag tctttatatg tagtcaaaaa
     4981 cttagattcc ataagctgag ataaaagctg ttgtaatgaa aaataattca tcatctcagt
     5041 ctctaatact acttgagtta tttgagaatt agttaaatcc attttaattt tttctaatat
     5101 atataaaatt aaaagtttgt gataggctaa ttcttcagac gagttttcaa acataaatat
     5161 catcctccgt caaactattt ctatatataa tatattaaac taatttgtac aaaattaaaa
     5221 gaacaatttg tattgtatta ttaatcttta ataattcata taaaaacaat tttataaata
     5281 aatatagaaa aacagggctg ctaattagca gccctgttga tacccaggtg tcaaaattta
     5341 ctatttattt tccagccatt tgtctttcag ccatttcaac taatttctta gtcatatatc
     5401 caccaacata tccattttct ctagctgtta agttaccttt gtctatgttt tcatagttag
     5461 ataaacctat ttcgttagct atttctaatt tcatttggtt taaagcagct tttgcttctg
     5521 gaactactgt tctgtttgaa tttgacatag taaatttcct cctcaaataa ttagtttatg
     5581 atgtctaaca acacttttat ttatgtgatg ttaatactaa tttctccaaa ataaaaataa
     5641 tatatacaga aaaataaagg taaaaatgta aaaaatataa aaaaattaaa taaatgagta
     5701 ttttagagag tttaattagg tcaatatagg ttttattttt ttagttattg tcttataaat
     5761 taaatattac agtacttttt tgagctgaaa aaaaataaat tttaaatata gaagaaatta
     5821 tttcaaaggg ggaatagtat gaaaagagaa gttaaaagga ctaaagcttt gatatatagt
     5881 gttttatttt tttcattatt attagtgtta atatttgttt ttgtttatgg aataaaatac
     5941 atatgttcta aatctgacaa ttcaaaaatg cctatatata aagttgatac aaatgagaaa
     6001 aaaatagctt tgagttttga tgtagcatgg ggaactgata atatggatga tattttaaaa
     6061 attttagata aacataatgt aaaagcgacc ttctttttag ttggtagttg ggtggatgat
     6121 aatgaagaaa ttgtaaaagc tatagatgaa aaaggacatg aaattggaaa tcattcaaat
     6181 actcatgcca atttaaagga aatatcaaaa gaagacataa caaaagagat agaaactaca
     6241 tcagaaaaaa tatataatat aacaggaaaa aggactaatt tatttagacc tccttttggg
     6301 gacgtaaaca ataaagctat ggacatttgt agtgatttag gttataaagt aattaagtgg
     6361 gatgtagatt ctatagactg gaaggagctt ggaccaaatc atgtaattga aaaagtaata
     6421 aaagaatctc aacctggttc aatagtttta ttccatgcta atataaatga tgtagataat
     6481 tatttggata ctataataag tcgactaaaa aaagatggat acagtctagt caaaatatct
     6541 gatttgttgt ataaagataa ttatgtagtt gattccaatg gagtgcaaaa aactaagaat
     6601 taataagtaa taaatgtata ttttaagtaa agttgtgata aattatatag tatatgttag
     6661 aaaagtgtcg ataaaaagac ggtgtctatt ttttgacact ttttatacat gtatgcaata
     6721 tcaataatta tagattaaat tgattaaaaa agtgtctaat aattgacttt tttttgatgg
     6781 gtttaaaaat ttagaagaat taatgaatgc tgtaagacag tatttccaaa gggtataagt
     6841 ttgtttaaaa gttggcatag aaattgcttt gttaataagc gatatgttct atagaaataa
     6901 acaatatgtt aagaccttat aatagaaaaa tagcattaac attatttgaa cagcttataa
     6961 aaaaattaat atacttaagg gggcgtacta gttgaacaaa ctatattcta ttttattaaa
     7021 acaacacgtt ggtggtccag ataagccagt agtaagtgtt ggagatgtag taaaaaaagg
     7081 aactctaatc gcagaaccaa ctggattagg ggcaaatatt tatgccagtg ttagtggaaa
     7141 aataagtgaa ataaatgacc aagcgatagt aattgaagct gatgaagtac aagaagatac
     7201 ttttgaacca ttaaaaggag aaggtatact tgacttaatt aaagaagctg gagtagtagg
     7261 aatgggtgga gcaggattcc ctactcatgt aaaattaaac atagatttaa atggtggaac
     7321 aatattagca aatgcagctg aatgtgaacc tttattagct cataatataa aagaaataga
     7381 agaaagacca gaaatagttt atcaaggtat aaaatatgct atggaagtta ctaatgctgg
     7441 aaaaggtatg ctagcaataa aaagtaagca tccaaaagca atagaagctt tcaaaaaagt
     7501 aataaagcca ggagacaaca tagaagttgc agaactagtt gatatgtacc caatgggaga
     7561 agagagagca atagtaagag atgttctagg aaaattactt gagccaactc aattaccttc
     7621 agaggctaat gcagtagtaa taaacgtaga aacactaact agaatagtag aggctgttga
     7681 gcaaaaaaga ccagttatat ctaaaaatat aactgtagtt ggtcaattaa acagtggaaa
     7741 agaatctata gtgtttgaag atgtgccaat tggaacaact gtcggagaat taattgaaag
     7801 agctggtgga ataaaaggtg aatatggtga aataatccta ggaggacctt tcactggtaa
     7861 agctactact ttagatgctc caataactaa aactagtggt ggtataatag ttactatgcc
     7921 atttgttaat gaaaagagaa aaatgggtct tttagtttgt gcatgtggac ctaatgaaga
     7981 aagaatgaga gatatagcaa ctaaaatggg agttactgat atagtatcag ttcaaaaatg
     8041 taaacaagct caagaaatta aaggtgcatt aaaatgtgaa aatccaggac actgtccagg
     8101 acaagctcaa aaatgtattg aatttaagaa agctggagca gaagttatat taataggaaa
     8161 ctgtactgac tgtagtaaca cagtaatggg ttctgcacca aaattaaaat taggtgtata
     8221 tcatattact gaccatgtaa tgagaacagt taatcaccca ttaataagaa gaataaaagg
     8281 tgacgtaaga ttaaaaataa aataatagat aaatagaagt tagacatctt tagaagtaaa
     8341 acttaaaagt tacttctaag gatgtttttt aatgtatttt gagggcaata tatatattgt
     8401 aaaataaatg gaaaaaatag acaaaatatg ctagatattg tataatgata acaatagcta
     8461 ttacaattaa tattggacaa aaataatgat actcaagtat gtggtttgta ttaatatgca
     8521 tattatatta agtcaactat ccgatagagt aaaaggtttt tgtgatattt gaagatactc
     8581 tatagtatct tttttattat aaaaactaat ttttgtcttt tattagataa gtatctagga
     8641 tactaattta tattttattg agttgttaaa aaagtgcact taaaaagtta aaaaagaaaa
     8701 attaaaaacc tatttataga aactgtgaag tatatcttaa acctgggcac ttaaaagata
     8761 tatggagtta gtagtgcaac ctgctataaa tataaatagg agtaactttt aattgtcaaa
     8821 aatatataaa ataaatacat aaataagtaa ttaatagaag atagatataa atatattttt
     8881 gaaatgctcc ctgcattcat aacgagtgta gagtggcaga tatctgccca aaactccttt
     8941 aattgctgga agttcctaaa gctaactaaa ccacaacata atcatgaaat aaggataagt
     9001 gtgatagagt gtgcatttaa taagcacttg aggttacgaa agtagaaaaa attagttaga
     9061 tagcataagg ttaaatccta agtgccttga aaatggataa tcagcaggta agcttcgaaa
     9121 agaagaaact tcaacgacta gagagaaatc tcgtacctac aagtgtgggg aaatggggag
     9181 tacctaattt atggttaggt aatgatatag tctgtgctta atagaaatat taagaagttc
     9241 ataggagaac tggttaggag taacgaccct aattgaacac actaaagatg tattattgaa
     9301 taagattcat ataatattta ataattttaa ttatattaag tgaatacaat atatgttttt
     9361 agtatcagga gaattttgaa aacaaaaatt aaaaaatcaa gtataatcag tttagtaatt
     9421 gcttttagta tggtatttac tgcatttaca ccaatagtat cttatgctga cgaagtgact
     9481 agcaatgata caatacttaa tggagaagaa caatcaaatg gtcagacatc agatgtagag
     9541 aaaccatcag acggtcaggt accagatggg gagaaaccat cagacagtca agtaccagat
     9601 gtagagaaac catcagacgg tcaggtacca gatggggaga aaccatcaga cagtcaagta
     9661 ccagatgggg agaaaccatc agacggtcag gtaccagatg gggagaaacc atcagatggt
     9721 cagataccag atggggagaa accatcagat ggtcagatac cagatggaga gaaaccatca
     9781 gatggtcaga taccagatgg ggagaaacca tcagatggtc agatgccaga tggagagaag
     9841 acatcagata gtcagacgcc agatgtagag aaaccatcag atggtcagat gccagatggg
     9901 gagaaaccat tagatgagca aactccagaa gaagaaaaac cattggaaga agaaatagtt
     9961 atagaggaaa tgagtttaaa acaagatatt gataaaattc ttgatatgac tttatctcaa
    10021 ataaataaaa tagtatataa cttttgggaa gatgaagaag atgtaaaggc agatgaacaa
    10081 tcagaaatta accaagtttt tacttcagaa gactcattta ttagcttatg gtatgacaaa
    10141 aaagcaaaag taaaaaattc ttgtttacta aaagaaatag atggttcaga tagaccttat
    10201 catgatttaa gatttgatga tattacaaag actttaactt ttgactatgt aataaagggt
    10261 cttgtaggag caagctcaaa agacgacaaa tatgtaatag aaggtgaaga tggagagcaa
    10321 actgcatttt gttacaataa tcatttaaga ccaccaagta gtaatggaaa gagtccatat
    10381 ttaccagcag aagattttaa tggtcaagaa aaccaaaatg aagaagcagt taaatctata
    10441 ctatatgcag gttctgaatt tgatggattt ggatataagc aacaatttaa tttaggtgga
    10501 gaagaaaatg aattgatgac ttactcagct actcaaagtg caatttggat tattcttgga
    10561 caaatggatg aacaagaaag acttaagcag tatcaaggtt ctataaacct ttgtgataaa
    10621 cttatagaaa gagcaaaaac tgaagaggaa agagctgagt atcagaagaa gaaagaagta
    10681 gcgataaata taaaagctta tttagaagca ttgttaaaag caggtcgtga agagttaaag
    10741 ccaaataata caggaaaacc tagtttgagt aatggtttaa ctaagattaa ctttgaaaaa
    10801 aatgaagatg gtacttatga aacagaagct gtagccttag taggttatag tggtgtagta
    10861 aagttaaaac taccagatgg agtaactgct tatgatggag atggtaatat aataggtaca
    10921 ggcgaagtag aaatttcaac gcaacagaaa tttaaactta agagtgttgg aaaaccagat
    10981 gccaaagcta atatttctgc tgtatcttat gattatattt ttccaaaagc tattcaatat
    11041 tataaggcag tgcttgattt aggacaaaaa gaccattcat cttcattacc atctagcaag
    11101 caaaatttat taagttatac aatagagaaa aagaatggtc aagaagttaa ctttaatata
    11161 ggtttaccaa cagatgatga tgatgttgta aatccaccag tgccaccaat agatgatgat
    11221 gttgtaaatc caccggtgcc accaacagat gatacaaatg gacataagcc aaaaccatca
    11281 ccaccaatag atgatacagt aataaatcca ccagtaccac caatggatga tacaataata
    11341 aatccaccag taccaccaac ggaggatgca gtgttaaatc caccagtgcc accaatggag
    11401 gatgcagtgc taaatccacc agtgccacca acagaggata cagtgttaaa tccaccagta
    11461 ccatcagtaa gtgatacagt ggaaaaaact ccagaactat caagagatga tacaataata
    11521 aaatcaccaa aaacaggtga tgaaactcag ctaatgtcat atgtatttat tagtgtcatt
    11581 gcaatttgtg gattagcgta tcaatgtaaa ataaaaagaa attaatctag attattatta
    11641 agtttaaaat aaaaagtctc aaattgattt ataagtcatt ttggggcttt tttatgtaaa
    11701 tataatatgc aaatatagtt tgtttaaaaa tatctatttt aattatattc ttaaatttat
    11761 tattggatac aggcattcat taattgtttg atgaatagaa aaatgttttg aaggtgaaaa
    11821 tagtattgaa aaaataatat act
//
LOCUS       DAMJVI010000106        11756 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.111, whole genome shotgun sequence.
ACCESSION   DAMJVI010000106 DAMJVI010000000
VERSION     DAMJVI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11756
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.111"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            114..1154
                     /locus_tag="PC545_003016"
     CDS             114..1154
                     /locus_tag="PC545_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457762.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFTRIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKILE
                     YFKLNNINIKLLEESDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1510..2832
                     /locus_tag="PC545_003017"
     CDS             1510..2832
                     /locus_tag="PC545_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2457763.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWAVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSIITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3129..4490
                     /locus_tag="PC545_003018"
     CDS             3129..4490
                     /locus_tag="PC545_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2457764.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGTQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSITSIISSLFAALPTGVFSQNTAIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4563..5957
                     /locus_tag="PC545_003019"
     CDS             4563..5957
                     /locus_tag="PC545_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457765.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKKVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMVELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNVILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6019..6489
                     /locus_tag="PC545_003020"
     CDS             6019..6489
                     /locus_tag="PC545_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HDF2457766.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLAKNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6492..8708
                     /locus_tag="PC545_003021"
     CDS             6492..8708
                     /locus_tag="PC545_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2457767.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGSLRFYEHKIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAVKLIKVEYEELPAVFTIQDAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKEIGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKKMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLLTGAHDMGNGVVTMQTMMVAEVLGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKNLILEEAEKLLETSKEDLYLDNGCVISKL
                     DENVKASLSDVAVYSQSKSLRELTVVEDYSSPAGRTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVDGYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8791..9462
                     /locus_tag="PC545_003022"
     CDS             8791..9462
                     /locus_tag="PC545_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2457768.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9825..11039
                     /gene="dpaL"
                     /locus_tag="PC545_003023"
     CDS             9825..11039
                     /gene="dpaL"
                     /locus_tag="PC545_003023"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDF2457769.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     DKLTSKEIKEKLGEITFFTATDGNHGRGVAWTANKLGQKSVVLMPKGSSEFRLNKIKG
                     EGADASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIMQGYGTMAQEA
                     IEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEADEADCYYKSAE
                     AGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHAAAFVSAPDWVSAKGMRTLGNP
                     LNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLISTEGDTDPDKY
                     RSIVWDGEYPSI"
     gene            11166..>11756
                     /locus_tag="PC545_003024"
     CDS             11166..>11756
                     /locus_tag="PC545_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2457770.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNRDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT         4440 a         1358 c         2390 g         3568 t
ORIGIN      
        1 ttttgtttta aaataatatt attaaattat aactaagctt ttctgttatg atataattaa
       61 atagataatg tgaatgatta ttatatagaa tacttatttg gagttgatat tttatggaca
      121 aacacagtga ttcagtaatg tatggtggaa tggaagtata taatatttgg ggaacaacaa
      181 cagtagactc cataattgga gagtctgatg taatgcgtgc tcttaagaat agaatttgta
      241 aaattggaaa tagtgattct actgtagcta taactggaga aagtggcact ggaaaagaat
      301 taatagcaag agccattcat ctcgcaggaa acagagggaa taaaacattc gttgctataa
      361 actgtgctgc tataccagaa cctttactag aaagtgaact ttttggatat acaaaaggag
      421 cttttagtgg agcagattca aaaggtaaaa ttggtaagtt tgaattggcc aatggagggg
      481 ttgtattttt agatgagata ggagatatgc caattcaact acaatcaaag ttattgaggg
      541 tattacagga aaagaagttt acgagaatag gttcaaatga actaattgat atagatgtta
      601 gaatcatatc agctactaat agagatttat ttgagttggt taaggaaaat aaatttagag
      661 aagacttata ctatagattg aatgtaatac ctttagaagt gcctccactt agagaaagag
      721 gagacgatat acaaatatta ataaacaatt ttatagacaa atattcgaga aagatgaaga
      781 aaaacgtgta ccatatagag ccagatgtgg aacaaatgtt gcttaagtat ccttggccag
      841 gaaatattag agagttggaa aatactatag aactggcatt aaaccttctt gaggaagatg
      901 gaataataca taaaggaata ataaatagaa agatacttga atattttaag ttaaataata
      961 taaatataaa gttacttgag gaaagtgact atgagataag tatggaacaa gacatactta
     1021 cacttgaaga gtttgaaaga gcctatataa agaaagtgtt aaagttttac ggaaatgata
     1081 ctaagtctaa aaaactggct gccaaaaaac ttggaatatc actagctaca ttgtacagaa
     1141 aaatagatgt ataataatta ggctttttat tctcaattct aaaatttgaa attataaaat
     1201 tgataaaaaa cacttggaaa actattttct aagtgttttt ttgtgtaata gcattttatt
     1261 atccaaaaat aaaaaaaatt taaaagaatt taaaaaaata aatgctttaa taaattatca
     1321 aatactaaca aatataataa aaattatata tgtaaaacaa ttatgttaaa aaattggcat
     1381 tataattgct tcatattaag ttgagatgac aaaataaaaa aagaaaggat gttgtgtgag
     1441 ataataataa ctgtcattaa aaaatagttg ggagtgataa ccttgagtaa atatcaaaag
     1501 aatgtttcta tgtttgatat tgatggaagg ccaccaattt taaagtgttt gccactatca
     1561 atgcaacata tactagcaat gatagttgga actgttacag tacctatagt cgtaggagga
     1621 gctgtgggtg ttagccctac acaaataaca cttttagtgc aatatgcatt aataatagct
     1681 ggtatatcaa cacttataca actatatcca ataggaaata ttggttcaag attgcctata
     1741 atttttggag ttggttttac ttatgtacca acacttgtag cagtcggagg aaattatgga
     1801 ttagcaagca ttttgggtgc acaattaata gggggagtgg ctacagtatt aatagggata
     1861 ttcataaaaa agataagaaa attttttccg aatatagttg caggaacagt ggtttttaca
     1921 attggattat ctttgtatcc tatagcaata aattatatgg caggtggtgt aaatagtcct
     1981 acttatgcct ctatgcaaaa ctgggcagta gcggctataa cattagtggt agttataggt
     2041 cttagtcaat ttgcaaaagg atatgcaaaa ttggcagcaa tatttttggg aattatagtt
     2101 ggatattgtg tatctttagt tctgggtata ataaattttg accctattag agaggcaact
     2161 tggtttgcga tacctaaacc gcttgaattt gggatggaat ttgatatgcc tgtaataatc
     2221 tcagtagtaa ttgtaagtat aatcaatgct gttcaagcaa ttggagactt atcagctact
     2281 acaatgggag gcatggatag gaatatcaca gataaggaat tatcaagtgg agtaatagga
     2341 agtggaattt gtactatgat aggagctttg tttggaggtt taccaccatc atcatatagc
     2401 caaaacgtgg ggatggtagc tatgaacaaa gtaataagta gatttgtgct tggaatagct
     2461 ggtatattta tgttattgtc ggggtttgtg cctaaatttg gtgcattaat gactactata
     2521 ccatattctg ttttaggagg agctacaata tctatattta gtataataac aatgacaggt
     2581 ataaagttaa ttattacaga agagttgtca tcaagaaata ttaccattgt tggtttggca
     2641 attgccttag gcatgggaat aacatcagtc ccagcagccc aagaggcatt tccttcattt
     2701 gtaaagttag tatttggaga atctccaata gtaattgcaa cattggttgc attcgtttta
     2761 aatctaataa taccaaataa aagtctagag gatgaggcta aagaaagagc tgttatggaa
     2821 gagactaatt aagatataat aatttaaaag aattgtttaa attatttagt atgagattgt
     2881 tagattattt ttgatagcta ttcattttaa taaaaaattt aacaaataaa tatataccag
     2941 gcaattaatg ttcatttaag ttgaaaatcg gggaggcaat atttagaaat ttgttaattt
     3001 tttataaaaa tagctcagtt tagctgtgtt tatcggttga gcaataaatt catggtcata
     3061 aaaatattta tagaaaataa ttataaaaat atttagtcaa gatatttatg tatgggagga
     3121 tttattttat gaaaacacgt aatacttcaa aatatgatgt cgatgggata ccaccactaa
     3181 aggaatcaat acctttggcc ttacaacatc tgttagcaat gatagtagga aatatggtac
     3241 ctgcgatatt aatagcaaac gtggtaggac ttaaccaggg gcaagcaaca atgttgattc
     3301 agggttctat gttagcagca ggtttagcaa cgtttttaca gttatatcca atacctttat
     3361 ttaaaggatt taagttaggt tcaagattac ctgtaatgat gggaatgagt tatgtatttt
     3421 taggagcttg tctttcagta gcagctaaag atggtttagc agctctattt ggagcacaga
     3481 ttgctgctgg ggtaatcgta ttctttgtag gatttggagt taagaaaatt agacatatat
     3541 tcactccaat agtttctgga actataatag catgtatggg acttggacta ttcgcaacag
     3601 ctataaagaa tttagctgga ggagaaggca cacaaacatt tggaagccca ataaacttca
     3661 cagtaggagt tatagttgct tttgtaataa taatgataaa taaatacggt aaaggtttag
     3721 taaaaaattc ttcaatatta ataggaatat tagtaggtta tgctatatct ttagttttag
     3781 gattggttga tttctcagcc gttcaaggag cagctatagt atctttacca acaccagcag
     3841 cttttggatt agaatttaga ccagagttaa tagtaatgtt tactattatt tacataatag
     3901 gtatagctga tatgatgggt gcctgtacaa tagctacagt aggagcaatg gatagagagg
     3961 taactgacga agaattagca tcagttgtat taggaaattc tataacttct ataatttcat
     4021 cactatttgc agctcttcca actggagtat ttagccaaaa tacagcaatt gtatctatga
     4081 acaaagttac aagtagattt gtaatagcac taggggcact tgtattatta ctagcaggga
     4141 tatctccagc attgggtgca ataatgacaa ctataccatc ttgtgttgtt ggtggagcaa
     4201 ctttagtagt tttctcatca atagcaatgt ctggtttctc tattatgagt atggatggat
     4261 tcactgaaga aaacaatctt atagcaggtg tatctatagc aacaagtatg ggtcttacaa
     4321 ctgctccaca agttttagac caattcccag aaacaattgg aactgtcctt ggagggtcaa
     4381 gtatagtttc tggagcaata atagctatat tacttcaaac attatttaaa ttaaagtcac
     4441 gtaaaagtgc tgaaaacgtt acttcaaatc ttgaagaaaa tattggataa atgtattaga
     4501 tataatatga gaaaaccata tggtaaaatg ttgtaatgaa agtattcaaa gggggattta
     4561 agatgattga tatactaata aaaaatgcaa taattgttac agttaataaa gacagagatg
     4621 ttatatttga tggagcaata gctattcaag gtgacagaat cttggacata ggaaatacag
     4681 ttgatatcga gcctaagtat cttgatgcta aaaaagttat agatgctaat ggaaaagtaa
     4741 tattccctgg ctttataaat actcataatc acctgttcca agtgttatta aaaggtttag
     4801 gtgatgatat ggcacttcat gagtggttaa atacaatgat gtttccatca gcaaaattct
     4861 taacagaaca agatacttat gatgctgcaa tgcttggatg tatggaaggt ttaaaaagtg
     4921 gtataactac aatggttgat tatatgcata ctcacaatag accaggactt acagatggaa
     4981 tagtaaaggc atataaagat ttaggcataa gaggggttgt aggtagaggt tgtatagatt
     5041 taggtattca caaagagctt atagaagatg tggaaacggt tgaaaaagat ttgagaaggg
     5101 tattcgaaaa atatcataat tcagaaaatg gaagaataaa aatctgtgta gctccttctt
     5161 caatgtgggc tatatctgaa gaaatgggta agatgctttg gaatatagtt aaagaatatg
     5221 attcatattt tacagttcat atatctgaaa cagaatttgc aagaacagca acaaaggata
     5281 tacatggaga aatagatata aaattacttg aaaaatgggg tatagtaggt ccagaagttg
     5341 tagcagtaca ttgtgtatgt ataactgatg aagacatgga aatgcttaaa aagtatgaca
     5401 taaaagtttc tcataatgta gcaagtaata tgtatcttgc ttctggtgtt gcacctgtgc
     5461 cagaaatgtt aaaaaaaggt ataaatgtga gtttaggtct tgacggtgca gcaagtaata
     5521 acgcacaaga tatggtagag cttatgaaac taacagcttt acaacacaaa gtaaataatt
     5581 gtgaccctct ggcaatttct gcagagaaag ttcttgaaat ggctacaatt gagggagcaa
     5641 gaactcttag aatggaagat gaaataggtt ctttggagat tgggaaaaaa gctgacctag
     5701 taatatttaa ctcaatgctt tctccaaaat caatacctct tcacaatcct gtatcaactt
     5761 tggtatactc agctagtatg cacaatgtag agagtgtttt agttgatgga aatgtaatac
     5821 ttgaaaatgg aaaagtaact acaattgaaa gtgaagaaca agtgttgctt aatgcacaac
     5881 atgcagcaga agaattatgt aagagagcta atgtaactaa tagaatggaa ggacataagt
     5941 ggaatagctt atactaatat taaaataaga ttgtaataat attctattac aacattagtt
     6001 tatctgtagg aggagaaaat ggaaaaaata aaattaaata ttaatgatta catgtatgaa
     6061 atagaagttg aaaagaatga aactcttttg catgtacttc gtgaaaaatt aaatctaact
     6121 ggagcgaaac aaggttgtaa tagtggcagt tgtggagcat gtaaagtttt gatagatgga
     6181 gttgacatga aatcttgtaa acttcttgca aaaaactgcg aaggtaaagt tatatacact
     6241 atagaaagtt ttgctaatgg agaaaatctt catccaatac aagaagcttt tatagaagtt
     6301 ggagctgtac aatgtggata ttgtactcct ggtatgatta taacaacaca agctctttta
     6361 aatagaaatg catctcctaa tgaagatgaa attagagaag cttttaaaga aaatctctgt
     6421 agatgcacag gatatgtaaa gattatagaa gctgtaaaat tatctgcagc tagattaggg
     6481 ggtagataat tatggaaaag gtaataggaa aatcataccc tctaaaagat gcagctttaa
     6541 aagttactgg tcaaatgaaa tacgtggctg atatgaaacc acaaaatgta ctttacgcta
     6601 agatgctttt aagccctgta gcacatgcaa aaataaaaag tatagataca agtgaagctg
     6661 agaaagtagt aggtgttaga gctatagcta cttatctcaa tacatcacaa agaaaataca
     6721 atggttcatt aagattttat gagcacaaaa tacctgaaaa tgaagttata ttttcagata
     6781 cagttagatt tgttggtgac cgtgttgcag cagtagctgc agagactcca gaaatagcag
     6841 agaaagctgt aaaacttata aaagttgaat atgaagaatt gccagctgta tttactatac
     6901 aagatgcaat aaaagaagat gctatctgca tacatggaga ttcaaatata gtttctcaaa
     6961 acaacataaa tgggggagat gtagaaaaag gcttcaagga atgtgactat atatttgaag
     7021 atgaatactc tactccagca atacatcata gtgcaataga gactcatagt gtaattgcac
     7081 actatgatta taatgataaa ttaactgtac atacaccttg ccaaaataca tttggattta
     7141 gaataatact atctcaaatt tttgggatgt cattaaataa agtaagagtt ataagacctg
     7201 ctattggagg ctcatttggt ggaaaattag aacttacaat tgaaccagta gcagccgcat
     7261 tatcaaaaat gacaagaaga ccagttaaaa tggttcttac aagaaaggaa tctatgatat
     7321 caacaagaac tcgtcatggg tcttttgtaa aattaaaaac tggtgtaaat aaagatggtg
     7381 aagtaatagc acaagacata aaaatatata caaatacagg agcatatgca tcaagtgctt
     7441 taaatgttat aggagcactt agtcataaag tatttaaagt ttataaaata ccaaatataa
     7501 agtttactgg gatgcctgta tatacaaata caccaatagc aggagctatg agaggctatg
     7561 ggtcacctca aatatttatg gctcaacaag ctcaatttgc gaaaatagca aaggaaatag
     7621 gtatggattt agtcgatttt caaaataaaa atgcagtaga gccagatgat gtcgatataa
     7681 tcttccacgg ttctcttgga aatcctagag tattggattg tatagagcaa ggtaagaaga
     7741 tgtttaagtg ggatgaaaag aaaaaacttc caaaagaaga aggaaaatat ttaagaggta
     7801 taggtatggc aataggtgca catggtaatg gagtttttgg tgcacataga gatgttgtta
     7861 cattgactct taaattaaat gaagatggta cgttgacact gctaacagga gctcatgata
     7921 tgggtaatgg tgttgtaact atgcaaacaa tgatggtagc tgaagtttta ggaataacac
     7981 ctgataaagt tgatacattt gaaactgata cggatgcatg tacattcaac cttggtgact
     8041 atgcaagtag aggggtattt gtagaaggtg gaggagctaa aaagacagca gagaaattga
     8101 aaaatcttat attagaagaa gcagaaaaat tacttgaaac atcaaaagaa gatttatatc
     8161 tagacaatgg atgtgtaatt agtaaacttg atgaaaatgt aaaagcatca ctttctgatg
     8221 tcgcagtata tagtcaaagt aaatcattaa gagagttaac tgttgttgaa gattattctt
     8281 caccagctgg acgtacttct tatggtgtac attttgctga agttttagta gataaagaaa
     8341 ctaaggatat aaaagtagtt gattttgtag ctgtacatga tgttggtaga gttataaatc
     8401 ctctaaattt agaaggacaa ttagaaggtg ggattcatat gggtctagga tacgcattga
     8461 gtgaagaatt ggcatttgat gaacaaggta gacctaaagc tactgatttt aaaacatata
     8521 aattatttag aactgtagat atgccaaaat gtcaagttgc atttgtagat ggttatgaaa
     8581 aagctgggcc ttttggtggt aagagtattg gagaatgtgc cgttgtacca gttgcgccag
     8641 ctgttgcaaa tgcagtatat aatgcaactg atgtagagat acattcatta ccaataaaat
     8701 taaaatagta taaattaaag cagaaataac aatttattgt atttgtaaat agataataaa
     8761 aatagattta tttggtgagg agatattatt atggcaaaga tattaggtat ttgtggaagt
     8821 ccacgtaaag gagcaacaga atatgcagtg ttagaagctt tgaaagaagc agaaactata
     8881 cctggtatag aaactgagta ttggtcagtt agagggaaaa aaataagtcc ttgtgtacat
     8941 tgtgatgcat gtataagaaa agaaactatg tgtattataa aagatgatat ccaagaatta
     9001 gagcaaaaaa tattggaggc agatggaatt atagtaggtt ctccagttta tgatatgaat
     9061 ataactgctc aacttacagc agtatttaat agacttcgtc caatgtattt agttcatcca
     9121 ggaaaacttc aaaataaggt tggtgcagct ataactactg gtggaactag atatggtggc
     9181 caagaattga caaaacttcc tataataaat ttcttcctaa tgcatgaaat gttagtgtct
     9241 ggaggtttag gtggatgcta tataggtggt acaatatgga gtaaagatgg aaaagcaaaa
     9301 ggtgcccaag aggatgagac aggtatggac acagtaaaaa gacttggaaa aggtgcagca
     9361 gaggcagtta tggtttctaa gtttggtctt gaaaaatgga atgtcgtaaa agaagaatta
     9421 gatgttaaaa aagatgaaaa atcacctctt agagaccact aaagatttat tatattattg
     9481 aatatataca aatagtgatg taatagatta ttgcatcact attttatgtt ttaatttaga
     9541 ttttatagat tttatattaa aaaatataga aacaaagtgt tttgagattt atttttaaat
     9601 tttaaaaatg agaattaaaa agtgagaaga tagaaaaggt tttttagtta ttaaattcta
     9661 catacattgt atgaactaaa gatagaaaag aaaaaataat aaaagcattg caatttctat
     9721 actaaagagt catagaaaaa tatctagtat atatttagtg ttggcatgtt aattgcttat
     9781 atattaaagt ataaaaattt aacaataatt tggaggtatt ttagatgtta aaagaaatta
     9841 aatggaaagt aaataatttg ccaaagggcg ataaggaaaa ttgtatcaaa tttttaaatg
     9901 aagaagaaat aactaaggtt agaaattttc acaaaagctt tcctcaatat aaagaaactc
     9961 cattagcaaa cctagaaggt ttagcaaaaa aattaggtgt tgcaggagta tatgtaaaag
    10021 atgaatctta tagatttgga ttaaatgcat ttaaagtatt aggtggttct tattctatgg
    10081 gtagatactt agctcaaaga ttagatacag atatatctga gttagggtat gacaaattaa
    10141 cttcaaaaga aataaaagaa aaattaggag aaataacttt ctttacagct acagatggaa
    10201 atcacggtag aggagttgct tggacagcta ataaattagg tcaaaaatct gttgtattaa
    10261 tgccaaaagg ttcttctgaa tttagattaa ataaaattaa aggagaaggt gcagatgcta
    10321 gtataacaga cttaaactat gatgatgcag taagattagc taatgattat gcagaagctg
    10381 atgaccatgg agtaatggtt caagatacag catgggacgg atatgaagaa ataccagcgt
    10441 ggataatgca aggatatgga actatggctc aagaagctat agagcaatta aaagaatatg
    10501 gagtagatag accaacacac gtatttgttc aagcaggagt tggttcactt gctggtgcag
    10561 ttcaagggta tgttgcttca atatatgatg aatgcccaat aacagtagta gtagaggctg
    10621 atgaagctga ttgttactat aaatcagcag aagctggaga tggaaaacca agatttgttg
    10681 gaggagatat gccaactata atggcagggc ttgcttgtgg agagccaaat actataggat
    10741 ttgaagtatt aaaaaatcat gcagcagcat ttgtttcagc accagactgg gtttctgcta
    10801 agggtatgag aacattaggt aatcctttaa atggtgatga aaaggtaatt tctggagaat
    10861 ctggagcagt tacaactggt cttttagtag cagctatgga aagagaagat ttagcagatt
    10921 taagaaaaga tttaaaatta gatgaaaatt caagaatact tttaataagc acagaaggag
    10981 atactgaccc tgacaaatat agaagtatag tttgggatgg agaatatcca agtatataag
    11041 gaattactaa aatgcaatta ttagatattg gtttacatat ttagaagata tataagttct
    11101 aaaatttaga aaatatataa gtatataaaa tacatacatt ataaaaaatc tagggggata
    11161 atgaaatgtt aaacgaacaa agaaaacaag aagtaactga aatatgccaa aaattaattc
    11221 aaaatcctag ttcttctgga aatgaagaag gtgttgtaaa agcaatagaa gagtcttttg
    11281 aaaaattagg ttttgatagt tggtcaagag atagatatgg aaatatagta ggatgtataa
    11341 aaggtaacaa gccaggtaag aaaatattat ttgatggaca tatagataca gttccagttc
    11401 cagatgcaag taaatggagt gttcctcctt ttgaaggaaa aatagttgat ggaaagatat
    11461 atggtagagg aacttctgac atgaaaggtc aatatactgc aatgatgtct gctgtagcgt
    11521 attttgctaa agacactaat agagattttg ctggagaact atatgtagca ggtgttgttc
    11581 atgaagaaat atttgaaggt gtttcggcta gagagataag taaggctgtt caacctgact
    11641 atgttgtaat aggtgagtct tcagagttaa acttaaagat tggtcagaga ggtagaggag
    11701 aaatagtagt agaaactttt ggtaaaccag ctcactctgc taatccagaa aaaggt
//
LOCUS       DAMJVI010000107        11606 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.112, whole genome shotgun sequence.
ACCESSION   DAMJVI010000107 DAMJVI010000000
VERSION     DAMJVI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.112"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            111..674
                     /locus_tag="PC545_003025"
     CDS             111..674
                     /locus_tag="PC545_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457771.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKLISDYRE
                     DYYYDTMALIYLLMNIQRQSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            1071..2771
                     /locus_tag="PC545_003026"
     CDS             1071..2771
                     /locus_tag="PC545_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457772.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVLHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQSFVTDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNDLKDEVLEGTVTRISPDVRGGNTNYYVTLDSNKDIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEKTGTDENTDSKDSENQGNV
                     "
     gene            2941..3558
                     /locus_tag="PC545_003027"
     CDS             2941..3558
                     /locus_tag="PC545_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457773.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3919..5112
                     /locus_tag="PC545_003028"
     CDS             3919..5112
                     /locus_tag="PC545_003028"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDF2457774.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5130..6422
                     /gene="lysA"
                     /locus_tag="PC545_003029"
     CDS             5130..6422
                     /gene="lysA"
                     /locus_tag="PC545_003029"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDF2457775.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYVEYFKAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSEYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIQLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHEAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEI"
     gene            6639..7298
                     /locus_tag="PC545_003030"
     CDS             6639..7298
                     /locus_tag="PC545_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457776.1"
                     /translation="MRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLEKHNYKK
                     AMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEKIKNGSS
                     VIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYSGSSKNN
                     KSSSKNNDKKSDSNKSDNSNSSSQNQEKPNNSNPTPAPTPQDTTGGSDSGGNSQNSNN
                     Q"
     gene            complement(7453..8538)
                     /locus_tag="PC545_003031"
     CDS             complement(7453..8538)
                     /locus_tag="PC545_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2457777.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEIKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYIDSFIEKNMSNIYFLKTLIPHIQHSLDNLLKEASNFVGKIPKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTARNKADNIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8729..9445
                     /locus_tag="PC545_003032"
     CDS             8729..9445
                     /locus_tag="PC545_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDF2457778.1"
                     /translation="MRINKYIASCGIASRRKAEEIILGGRIKVNGSVVKELSFNIDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVRERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKMYVASVKGIISDDEIRQFETGLKIEDYTT
                     APAKIRVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9503..10081
                     /locus_tag="PC545_003033"
     CDS             9503..10081
                     /locus_tag="PC545_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2457779.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10475..11347
                     /locus_tag="PC545_003034"
     CDS             10475..11347
                     /locus_tag="PC545_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2457780.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
BASE COUNT         4788 a         1204 c         1990 g         3624 t
ORIGIN      
        1 aagtacctta aaatcaaaat ttgtaaaacc acatttatta tgttagaata tcttatgata
       61 ttaaattgta agtgtataat attatttagt tactagattg gggggaaatg atgaataaag
      121 tctatattgt aacaacatat acaggaacta tattatctta cttaataagg aatataagta
      181 aaaagctgta tacacatgtg tcaatatcat taaatgaaaa tctaaaacca atgtattctt
      241 ttggaaggct aaatccaagg aatccattta ttggaggatt tgttgaagaa aatataaatc
      301 aaggattata tgctataaga aaaaacactg tgtgtagggt atattcgtta gaagtagata
      361 atttacaata tgaaaatcta tataaaaata taaaattaat tagtgattat agagaagatt
      421 actattacga tacaatggca ttaatatatt tactaatgaa tattcagaga caatctgaat
      481 ataaatatgt atgttcaaat tttgttgctg acatgttaga aaaaagtgag atagacattt
      541 taaataaaca agcatttgaa gtaacgccaa atgattttta taatttatct ggcttaacct
      601 tagagtatga aggtttatta tcagaatata attctagaca acattcttat gtatcaaaaa
      661 tcgcaaacat ttaaaaactt gatttagagt ttttcatata aaaaaatatt gctagataat
      721 taggctgaag atagtagttt aacttcgata ggattaagaa tttgtaaatc ttagttagaa
      781 tactttcttc agtttttaaa tttctgctaa tatttgtatt tttataatat ttttggatat
      841 atataataca gaatagtgca aaaatcccac tcctttaggt gcgggatgga tagtacaaac
      901 agtgtataaa aagtatttat acatacacgt ataatgttaa atatagttga ataagcagaa
      961 ctataaatat ataaagatat cttaatatat aataaatatg ataaatattt attttttgta
     1021 aatatatgga tataatagta ataataacat tttttagaga ggagtattaa atgaaacgat
     1081 atcttaaaac atatggagtt gcattaataa ttgtaataat ttatgcattt attaaattac
     1141 ctgtactaag attagatttt acatctttat tttcagtagg aataattttc tttgttgtag
     1201 caggaatact agatatgatg ttagatagaa atgaaagagc atctaaaatg gctaaatata
     1261 atttttcaat agcaatagct ttgcttgtgt atattgtagt agttcccttt ataacttcta
     1321 cacctgtact tcatgcaaaa tcatatagag aactattggg aaaagtaact gaaagtaaat
     1381 ttactgatga cattagccct gtcagtgtaa atgacattcg tcttgtagat gaagatatgg
     1441 caatgaaatt aggtgacaaa aagcttggag aagttcctgc aatagggagt gtatccaaat
     1501 taggaaaatt tcatatacaa aatgtagatg gagagttgta ctgggtagcg cctcttgttc
     1561 acagagacat aattaaatgg ataacatctc taagtggtac aagtggttat gtaaaagtat
     1621 cagctagtaa tcctcaagac gtccaattag ttcaagaaat agatggaaag ccaataaaga
     1681 tagtttacca accagaagcc tacttacatc aggatttaca aagacattta tacatacatg
     1741 gtattgtaaa tgttggtatg acagatttca ctcttgaaat aaatgatgaa ggaagacctt
     1801 attgggttgt aagtttatat gaacataaaa ttggctatgg aggagcaaat gcaacaggaa
     1861 tagccactgt tgatgcagaa actggaaaaa taaatgttta tgatgttaaa aacacgccta
     1921 aatgggtgga tagaatacaa ccacaaagct ttgtaacaga ccaaattaaa gactggggtg
     1981 tttatgttaa tggattttta aactctgtta tatcagaaaa gggagtttta gtaccaacag
     2041 aaggtacatc actagtttat ggcaatgata acaggtctta ttggtataca ggtataacat
     2101 cttcaggtgg agatgaatct acaattggat ttatgttagt tgattctaga acaaaagaag
     2161 ctagattata taaacaacca ggagctactg aaacggctgc aatgaaatca gctgaaggta
     2221 atgttcaaga aaaaaattat gaagcgacgt tccctgtaat gtataatata ttgggtcagc
     2281 caacatatgt atcatcatta aaagataagg ctggtcttgt taagagagtt gcatttgtat
     2341 ctgttgaaga ttataatgta cttggaatag gtgaagataa gaatgaagca ttaagaaact
     2401 ataaggatgc attagaatct aagggtaatg atttaaaact tgataatgat ttaaaagatg
     2461 aggtattaga aggcacagtg acaagaataa gcccagatgt gcgaggtggt aacactaatt
     2521 attatgtaac tttagattca aataaagata taatatttag ggctacttca aaagtatcta
     2581 gcgaattgcc attgactcag gttggtgata aagtcaaaat atcgtattca aaagaagaat
     2641 ctggtgtaat agaaatgtca gaatttgata acctaaatat tgcaaacttt gaagataaaa
     2701 aagcaaaaga aaaaacagga actgatgaaa atacagattc caaagattct gaaaatcaag
     2761 gtaatgtata atttaaataa aaagattgat aatagtattt aacatagtga tagctaattt
     2821 ttatagaatt taattctata aaaattagct attttatttt aattctttgt aaatttaatg
     2881 tcataaaatt aatataatat tagtacaact ttgcaaataa tacttcaagg agaatgtact
     2941 atgacgaata tggaaaaaga gatattactt ttaattagca gggttagatg tgttacagag
     3001 tctcaattta gaaaaattta tggagaccaa aaaagataca ataaaaaaaa ttttaaaaaa
     3061 actttgagaa aaatgtgtag tgaatataca ctaaaaaaat atccttgtga tgtggattat
     3121 tataattata aagacctctc ttatgtatac tatttaaacg gtagtaaact ttttaaggga
     3181 gatgatttaa taaaggctct agtaggatca gaattagttg ttagaatcaa tactgcaggt
     3241 tgtgatgtaa aaagatttta ccgaaatgtt agtgtagatg atgtgaaata cgatttattt
     3301 attgaatata cagaccaatt tggtaatata aagcaagttt tagttgatgt tgacttaaat
     3361 aatgaaatag acatttctaa atacgaaaat ctttctaaaa aaatagataa atctacaatt
     3421 ccatttttta aagtaccaaa cgttttggta gttacaccag ataatctatc cgaattaaat
     3481 gatttagaga gatatgataa tgagtatagc ataaatttta tagatttagg attaaataaa
     3541 ctatttaatt gtatataagc taactttaga aaagaaaact atcgatttgc cacattttgt
     3601 aaaataattg ttgcaatatt atatagaata tgataaaact atattaataa attaattaaa
     3661 aataatgagc ctagggtaga ggtagctgta ttcaatagta ttcaatagga gctggtaagc
     3721 gttgataatt gatgaaaggg tatatagccg aagaaatttt gttgacggac aaattttctg
     3781 gacttatgca gaatatgcat aaggctgtca cttttaaagt ggtgagctac aaggttactt
     3841 ggtctatgaa atttacacgt ccaaagtata cctttgggcg tttttattta tcaaaattta
     3901 gcttgagggg gagaaagcat gagtataatt gttcaaaaat atggtggaag ttcagtagca
     3961 gatactgaaa aaataaaatc aatagcagaa aatataatag aacgaagaaa agaaaatcca
     4021 caaatggtaa tagttgtgtc tgccatggga aaatccactg atgaatatat aactttagca
     4081 aaggaattga gtaatgaacc tagtaagagg gaattagatg ctttaatgtc tactggtgaa
     4141 atgatttcag catctttatt atcaatagca ttaaatgcac ttggatgtaa agctataagt
     4201 tataatgctt accagttaaa tataaaaacg agtggtcttc atggaaagtc tcaaattgat
     4261 gatataaacg taaacagaat taaaaatagt ttagatgaag gaaatgtcgt gatagtaact
     4321 ggatttcagg gaatcaacga agatggtgat gtaaccactc ttggaagagg tggttcagat
     4381 acatctgctg tagctcttgc ggttaaatta aatggaaaat gtgagatata tacagatgtt
     4441 gatggaattt attttacaga cccaagaaaa tattcaaagg ctagtaaact tgatgagatt
     4501 gaatatgaag aaatgcttga gttagcgagt ttaggagcac aagtaatgca ttcaagaagt
     4561 atagagcttg cacaaaaata tggtgttgaa atctatgtcg gacgtacatg tggtacagaa
     4621 aaaggaacat atataagagg gggaaaagac atgaaattag aggataaagt aataacagga
     4681 ttagctacta gtgatgatga cagttctata acaataaaag attttaaagc tgaaaatata
     4741 tctagcttat ttgaagatat agcaactata ggtataagtg tagatatgat aagccaaact
     4801 gcacctattt tagataagat aagtgtatca tttacagttc caaaagaaga gttgggagaa
     4861 tgtaaaaaga tagtatctaa atatacagat gaagaacatg tagtgattga taataatatc
     4921 actaaatttt ctttagtagg tcttggtatg aaaaatactt ctggagttgc tgctaaagta
     4981 tttaagatat tcaatgaaaa tggtataatg ataaaactta taactacatc agagattcgt
     5041 ataacttgtg ctataaattc tgatgataag caagtagcta tagaaaaaat agcagaagta
     5101 tttaatatat aaaatttggg gaggctatta tgaaacttca tggaactatg actgtaaata
     5161 atgatgaatt atatattgga ggagtgagtt gtttagaact tagtaaaaaa tatcaaactc
     5221 cactttatgt atttgatgaa gctttagtta gacaaaattg tagagaatat gtagaatatt
     5281 ttaaggctag agagggaaaa aataaggttg cttatgcagg gaaagctttt ttacctcttt
     5341 acatgtgtaa tcttataaat gaagaacaaa tgtgtttaga tgtagtttca ggaggcgagt
     5401 tatatacagc ttataaatca aattttccta tggaaaaagt tttctttcat ggaaacaata
     5461 agactaaaga agaaataaac atgggattag atttaggtgt aggtaaattt gtggtggata
     5521 atttttatga attagatttg cttgaagcat tatgtgaaga taaaggtaaa actcaagaga
     5581 tatactttag aataactcct ggtatagaag ctcatacaca tgattatata aaaactggac
     5641 agatagattc taaatttgga tttgctttag taaatggaga tttatttaga gcaatagaaa
     5701 aattaagtga atataaaaat attaaacttg tgggtcttca cgctcatata ggttcacaaa
     5761 tatttgatgt agaaccatac atagatacag tggatataat gatgaacttg gtaaaagaaa
     5821 taaaagaaaa attcaacata caactaacag aaattgacct aggtggagga gttggcgttt
     5881 attatagcga aggagataaa cctaaaacta taaaagagtt ttgtgaaacc ataatcacta
     5941 aagcagaaac ttcatgtaaa gagcttggaa tagaagtacc tacattggta atagaaccag
     6001 gaaggtcaat agttgcaaat gcaggaagta cattgtatac agtaggttct ataaaagata
     6061 taaaagatgt tagggtttat gtaagtgtag atggaggtat gacagataat gtaagacctt
     6121 cgttgtacca agctagttat gagtgtagta ttgtgaataa aattaatcat gaggctatga
     6181 atcatgtaac aatagctgga aaatgttgtg agagtggaga tatattaatt ggagatataa
     6241 acataatgga tattaaaagt ggagatatac ttataacaac atcaactggt gcatatggat
     6301 attcaatgtc ttcaaattat aataaaatac caagagcagc agtagtatca gtatttgatg
     6361 gaaatgataa gataatttgt aagagacaaa gctatgagga cttattagct ttagagatat
     6421 aaaatggaga agtatataat aaaaattaaa tattaaagtt tattatgagc agatttgcta
     6481 ggatatgtat ctctaaatta atttttggtt tattgtatcg ttcgtagcat atctgcttat
     6541 tttttgtttt actaacaaat ttcttgtaat aaatataaaa tattttaaaa actttataat
     6601 tatgataaaa tttaaagtat atgacaagga gggatttaat gagaggaaga gtaattttat
     6661 taatatcgat attatctata tttcttgtag gatgttcatt taataaaaaa gatgaaataa
     6721 atttagtaga gcaaggaaaa acatatttag aaaaacataa ctataaaaaa gctatggaat
     6781 cattatcctc tgcactggaa gaagatagta ctaatgagaa tgcaagagct atgtatatgc
     6841 aagctatgag aatgtcaaat atgacggagt ttgaagaatt aaaaaattat gagggtgcaa
     6901 tcaaggaact taaacttata gagaaaatca aaaatggttc ctctgttatt aaagaagagg
     6961 caactaaaaa gaaagaagag ttaactaaat tagaaaaaga acaaaacgaa gcagaggaac
     7021 aaagaaagaa aaaggcaaaa gatacggctg gagaagatag gtatagagtt gaatcagatg
     7081 caaggaaatc ttcttatagt ggaagtagta aaaataataa atctagttct aaaaataatg
     7141 ataaaaaatc agatagtaat aagtctgaca atagtaacag ttcttctcaa aatcaagaaa
     7201 agccaaataa tagtaatcca acaccagcac caactccaca agatactact ggtggttcag
     7261 atagtggtgg aaattctcaa aactcaaata atcaataagt ataaaatata tgcataaatt
     7321 taatgaacaa attatatatt ataaaaaata tctcttagca attttttctt actaagagat
     7381 attttttgag aaataattta ataaataaca gttaaatttt aaactaaaaa ttaaagtcac
     7441 agtagagata agctattgta aacgattatc tatatattta tcaacataag tttttacaac
     7501 agcagctatt ggtactgaaa gaaaaactcc tatgaaacca aatagagatt gtcccaccaa
     7561 aatagcagat attacccata tagctcctat accaacttga ttacccataa cttttggttc
     7621 tataaaattt ccatctaatt gttgcaatat aaatagaaat gcaatgaccc atatagtttt
     7681 ttgtggacta tataatatag tcatggctat tggagggatt gcaccaataa aaggaccaaa
     7741 atagggaatt atattaccta agaatattat tgaagctaaa aataaagcat tttctattct
     7801 gataacaaat tgaaatccta aaaatgcaat tataccaata ataagagagt ctaatattct
     7861 accaagtaaa taatcataga aaatatcatg agtagtccta aaaaattcta taatgttatc
     7921 tgccttgttt ctagcagtag aagaatatag aaatctctta aatcccaatg caattttttc
     7981 tttgtcatat ataatgtata tagatagtat aaatcccatt gtcatattaa atagcataga
     8041 agatatacta agtgtataga ttaagaagtt tttaggaatt ttaccaacaa aattacttgc
     8101 ttcttttaat aaattatcta aactatgttg tatatgagga attaaagttt ttagaaaata
     8161 tatgtttgac atattttttt ctataaaact atctatataa tcagtataca taggcatttc
     8221 atttatcaat ctatttaatg tattagctat tgatggaatt aataacctaa tggcaaaggc
     8281 taaaataaaa cctataaata tataagataa aaaaatggct aaaagtctat gtgctttgaa
     8341 ttttatctca aaaagcatca caagtggatt taaaagtaaa gcaagtaaaa tagctaataa
     8401 aaatgggcta aaaaacttta agaatccttc aatactagat ataaagtctg atggagtatt
     8461 aataaattta aaaaataaaa ttccaataaa cacgactatt aaataatatt ttatattttc
     8521 ctttgaaata ataatcactt ccttaaaata gtgtttagtc agctattaca tattaatagt
     8581 atgtgaaaag aaattacaat tatttaggaa aattaaataa tttttttgct tgtttttaat
     8641 taaggtctag tatagtataa taatgtagca aaattaacat tgataaaaat attaaacaca
     8701 cataatttaa gataaggaag gattaagtat gagaattaat aagtatatag cttcttgtgg
     8761 aatagcatca agaagaaaag cagaagaaat aattcttgga ggaagaataa aggtaaatgg
     8821 aagtgtagta aaagagttat cttttaatat tgatgaagaa aaagacatag tagaatttga
     8881 taataaaaaa gtaaaaccaa gtgaaaatta tatatatata gtattaaata aaccagaggg
     8941 atatataact acagtaaaag accaattcaa tagacccagt gtaatagaca tacttaaaga
     9001 tgtaagggaa agagtatatc caatagggag attagattat gaaactagtg gtttattaat
     9061 acttactaat gatggcgatt taacatataa gcttacccat ccaaagcatg aaattgataa
     9121 aatgtatgtg gcaagtgtga agggaattat aagtgatgat gagataagac aattcgaaac
     9181 aggactaaaa atagaagatt acacaacggc acctgccaaa ataagggtaa ctaaagaaaa
     9241 taaagaaaaa aattattcag tgtgtgaaat aactatacat gagggtagaa atagacaagt
     9301 aagaaaaatg tgcaaagcta taaatcatcc agttttaaat ttaagaagga tttctgtggg
     9361 gaaaatagta ttaaaagaca ccaaagttgg agaatataga tacctaacag aagatgaaat
     9421 aaaatattta aaatcaataa aataagataa aaataaattg agaaaaaatg aaataaagtt
     9481 aatagtaggg agaaatagta ttatggcata tgcaaagtat ttaactaaaa ttacagatat
     9541 aaataaattt tatttagaaa atattataaa tgaaggtgac atagtaattg atgctactat
     9601 gggaaatggg tatgatacaa ggtatctagc tgagaaggtt ggagaaaagg gctttgtata
     9661 tgcttttgat atacaagaag aagcaataaa gtctacaaga aagaaactag agaaagaagg
     9721 atatacagat agagtaaaat tgatatttga tgggcatgaa aaaatgaatc aatatataaa
     9781 agaagaagtt tcatgtgttt tatttaattt gggttatctt ccaagagcga aacattcaat
     9841 tataaccaaa ccagatacaa ctttaaaggc tataaagtta agtttagaaa tgttaaaaga
     9901 aaatggagta ataagcattg ccatatatac agggcatgat ggaggtcaag aagaaaaaga
     9961 ttgcatatat aaccttgtca ataatttaaa tcaagatgaa tttaatgttt tagaatctaa
    10021 gtttttaaat caagtcaata acccaccaca attgatttta attgaaaaga aaaaagcata
    10081 ataaatacta aaaaacttga caagattgta ttaagagtta atatatatag tgtgacctat
    10141 taaaaatact ttaaataata agtatacaac agtttattct caaaacaaaa tatacatcta
    10201 aatatgcaaa ataagataga taacttttaa atgagtaaaa attgaaaaat ttaaaagaaa
    10261 tattggataa atattaaaaa atgcaaggaa agatggtata ataaatttaa tcgaaaatac
    10321 tatgtgaagt ataaggtaca gaaaagttag aattttatga gtttaagaag ctatgataaa
    10381 aactaagatg aaattgagat aaaaaataaa atttttagag aaaaagacag tgttaataaa
    10441 acaaaatagt ttatataact aaaaggagaa aaaaatggaa gaaacaaatg aaatgaagga
    10501 cagtaaacat ttgatatcaa atatacaatc tcaatatacg agattgagta agggtcaaaa
    10561 acttatagca caatacattt taaataatta tgataaagtt gcatttatga ctgcatgtaa
    10621 attgggtgaa actgtaggag taagtgagtc tacagttgta agatttgcaa atgcattagg
    10681 ttatagtgga tatcctaaat tacaagctgc acttcaagaa ctcataaaaa ataaactaac
    10741 tacagtacaa agagttgaaa tggcacatga ttattccgat gattttgcaa tactaaacaa
    10801 agttttaaaa agtgatatag ataatataag gtctacacta gaagaaattg acgagagagc
    10861 gtttaaagaa gcttctaaca aacttttaag agcgagaaaa atatatatac ttggaatgag
    10921 gagttctttt gtagtagctc aatatttagg tttttactta gatataatac ttgacaatgt
    10981 tcatataatt agaatggata tgggagatgc ttttgagcag attgtaagaa taaatgaaga
    11041 agatgtaata gttgctatta gtttccctcg ttattctaag aaatcttatc aaattgtcaa
    11101 ttatgctaaa gaaaaaggag cccatgtaat atcattaaca gatagcttat ttgcgccagt
    11161 agcatcactt gcagataaca ctcttctagt taagagtaat atggcttcat ttgtagattc
    11221 tctagttcca gctcttagta tttcaaatgc tcttgctata tctgttggga tgaaggaaaa
    11281 agaagatatt aaacagcatt ttgatgattt agagcaaatt tggaaaagat attctgtgta
    11341 tgagtaaagt ttgtgtatta ataaggctat gaaattgaga atcatagcct tattaataaa
    11401 tactagatta tcacttaaaa aataaatttt cgtttaaata atacaaataa atatttcgag
    11461 actcaaaaca taaagatgta ttctgaatat atctttacta catcaactaa tttattaaag
    11521 aaagcatttc ccttcatgga gagtgctttt tttatttctc taaacaatat catttcctta
    11581 attaacaatt ataaaattaa ttttat
//
LOCUS       DAMJVI010000108        11598 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.113, whole genome shotgun sequence.
ACCESSION   DAMJVI010000108 DAMJVI010000000
VERSION     DAMJVI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.113"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            188..358
                     /locus_tag="PC545_003035"
     CDS             188..358
                     /locus_tag="PC545_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2457781.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDAGSCIDCGSC
                     AGACPVDAPQPE"
     gene            complement(534..1073)
                     /locus_tag="PC545_003036"
     CDS             complement(534..1073)
                     /locus_tag="PC545_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2457782.1"
                     /translation="MVFVEELWKKAIEFHGHECPGLAIGFKAALAAKKYLGCKQSGDE
                     EIVCISENDACGIDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKRYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFDKYERFYEEY"
     gene            complement(1212..1844)
                     /locus_tag="PC545_003037"
     CDS             complement(1212..1844)
                     /locus_tag="PC545_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDF2457783.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            1980..3893
                     /locus_tag="PC545_003038"
     CDS             1980..3893
                     /locus_tag="PC545_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2457784.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIEMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELQPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLEIEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            3909..5285
                     /locus_tag="PC545_003039"
     CDS             3909..5285
                     /locus_tag="PC545_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457785.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVSNIRKVTGLTCVLA
                     IVLSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAVSTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYLYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAIGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(5425..6075)
                     /locus_tag="PC545_003040"
     CDS             complement(5425..6075)
                     /locus_tag="PC545_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HDF2457786.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCISNKQENNVCNIKAI
                     RNFSEIYSISRKSRYHSLLKLTEKEIVKKMGNTYEILNYDELHKLAFEVKEFLEDE"
     gene            6546..8558
                     /locus_tag="PC545_003041"
     CDS             6546..8558
                     /locus_tag="PC545_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457787.1"
                     /translation="MKKSVALVNDSRKDLIDFLENNLKLVFGDSININRYFINEINDN
                     DIINDDVILVMSVERLDKIINNILDKKKVIVVRRTFREDKIYNLLSLPQGTNVLIVND
                     SDETTLETISLFYKIGVTNIRPIPYMNDNNYKNIKIAITPGVPEKVPSFISDIFDLGH
                     RYIDISTFIEIINLLQIDSKEIQSNLVKYSEEIISLDTGIKDKYKELFLKIEELDTIL
                     NLSKDGILFTSKDGEINTYNSKVKDILDINEDIYGKYIEDIFVDSLKVLLSEKEILDK
                     VVVFNKKYINVNKKNIYNRDEKMGTYYSLQEITYIKKLEQNLTKKLREKGQIAKYTFK
                     DIKTNSPKMFECIDLAKKVSKSDLSILIRGESGTGKELIAQSIHNNSNRKNQPFIAVN
                     CAAVPENLLESQLFGYDKGTFTGGLKDGKQGLFELANNGTIFLDEIGDMPLELQTKLL
                     RVLQEKQIMPVGSHNVINIDVRIISATNKNLEQMIDNSQFREDLYYRLNTIPINIPPL
                     RERKEDILIIMEDLINKKLVITPEAKKLIQNYMWKGNIRELQNVTSYLNIMCEDIVLE
                     KDLPPNLRSSDNKNTSLKLKYSKNDILNILEILILNKESDVGIGRGLILKALLDKNLQ
                     ITEGKIKKIFEYLKKEELIICSSGRYGSKITQKGEDFYNKLKYKGL"
     gene            8927..10147
                     /locus_tag="PC545_003042"
     CDS             8927..10147
                     /locus_tag="PC545_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457788.1"
                     /translation="MQQALKLKYQTKELGKDFVIESCNSIKPWLINIRRELHKIPELA
                     LEENLTKQKVISYLKEIGIDYMEFTKHNGIMAYILKESADKTICIRADMDALPIEEEN
                     NIPYKSIHSGKMHACGHDAHTTMLLGACKVLHSIKDKLNVNVKFLFQPAEEGFGGAKF
                     LVEDGCLENPKADYIFGLHVMPHIETGLIETKYDTLNASVDTIKISIKGKRAHGAYPE
                     NGIDAIVTASQIVTSLQTIISRNLEPNNAVVLTIGKIYGGDAHNVICEDVKLEGTLRT
                     LNSKTRNFMIDKIAKIVGDTASAFGCVGTLHVSDENYPAVINEKELVDTVISNTKELL
                     GEEKFILRPNPSLGGEDFSFYTEHCKGAFFHLGCKNEEKGLISPLHTSSFNIDEDCLP
                     IGVMMHVMNTLYFN"
     gene            10184..11533
                     /locus_tag="PC545_003043"
     CDS             10184..11533
                     /locus_tag="PC545_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2457789.1"
                     /translation="MEKAKKLGLFSMILLGINSIIGSGIFLLPGKVYNLAGQNSMFIY
                     IFATLLVLSILLCFAEVGSMFDKKGGAYLYSKKAFGDFIGFEVGTMSWVIRIISWSTL
                     AVGFATALGSFWPESATEYKGYIAAILVTLLSINSLFGIKSTKIMNNVITIAKLVPLI
                     VFIIVGIFFIKFVNIVPSGNVVNSSMGPAIILVFYAFTGFESFIVASGEMENPKKNLP
                     VALITTIFICAIIYILIQIVCMGILGDRLFENSIPIADASSVFVGNYGKVFISVATLI
                     SIFGINIGSSIVTPKCGSSLAEEGSLPAFIGKTNKYGAPYVAIIISLICCIPLVLTGS
                     FEQLAVMSVIARFAQYIPTCLSVIVLRKRTDVKASFKIPFGPVIPIVAILGSLWLLQQ
                     AWAEDISKPITQNRVLIGLGAMLLIAPLYIFMKKNKEIEHKNKSEDSKLYISREKIQ"
BASE COUNT         4400 a         1457 c         1671 g         4070 t
ORIGIN      
        1 attttatttt taaaataaac ttctttaaaa taaataaaaa tataagcaga ttaaacttta
       61 ttgtaaaact ttaaaacaag aatatcataa taaagttttt gttgtagaat taatttaatt
      121 gttatattca ataatatttg ttataatgtt attgaattac aatacatata aaagggagga
      181 tttcaaaatg gcatataaaa ttactgacgc ttgtataagc tgtggtgctt gtgaggctga
      241 atgccctgtt agctgtatat cagctggaga cgacgcatat gttatagatg ctggttcttg
      301 tatagactgt ggttcttgtg caggagcttg tcctgtagat gcacctcaac cagaataata
      361 attcgttatt cataaagaga ctgattttat tttataaata ctaatcagcc tctttttaat
      421 tatgtatgat tcaattaata ttatttatat actttattat gttgctacct aattcatctt
      481 aaaatactgt tagcacatat ctaatacttt taactttaat ctaatttata attttaatac
      541 tcttcataaa atctttcata tttatcaaaa caatcaagac atactttctt tccttcttca
      601 atccttatat attgctcaga tgcaaattca ccacacactt cacatttaag ggatttaaat
      661 attcttgctt tttcaggaag tcttaactta gtatctgtaa tctcaaatat ttcttcaaaa
      721 tcactttcta ataaatatct tttgtatcct tctctatcca tctcaaatgg ttttgacttt
      781 acatatatcc ttatagattt tccattttct ctattaaaga aatgatatac tgattttcca
      841 gtcatcctaa gtagtaaatt tcccttaccc attgtgcaac ttagcactac ttgtatacca
      901 tctattccac aagcatcatt ttcagaaata catacaattt cctcatctcc tgactgttta
      961 catcctaaat atttttttgc tgcaagtgct gctttaaatc ctatagctaa ccctggacat
     1021 tcatgaccat gaaattcaat tgcctttttc cataattcct ccacaaaaac cactctcctt
     1081 aatattgttt atttccatac attatttaaa tttttatcct aatatttcaa aaagccagag
     1141 gatttaatat taaatcttct gactttctca atactcttta caactgctca ttattaatat
     1201 gtttaagttg tctactttcc ttctttcatt aaatacgata aggtaaataa actttctgtt
     1261 aagtttacat tttcaactat aacacctttc ggaacttcta aatctaaagg tgcaaaattc
     1321 catacacctt tgattccagc ttttacaact ctattaataa cttcttgtgc tccattttta
     1381 ggtatacata atacagcaat atctatatta ttgtttttta taaagtcttc taaagtatct
     1441 gaatctaata cttcaaactc tcttatcttt aaacctatca ttctaggatt tgcatcaaat
     1501 aaagctttta tctcgaatcc agcttttcta aatcctgcat aattggctat agcttgtcct
     1561 aagttacctg ctcctacaag aactgcgttg tatggtcgat ccaacccaag aattttacct
     1621 atctcagtat gaagagcttc tacattataa ccatatcctt gttgtccaaa tccaccaaag
     1681 ttgtttaaat cttgtcttat ttgagaagcg gtaaacccta ttatatcact caattcttta
     1741 gaagatattc tttgtatatc cctatctaat aagtctccaa gatatctatg atattttggg
     1801 agccttctta taactgccat tgatatattt ttatttccca acatcttcac ctcctaaaag
     1861 tgtcctcttt tttgtttgat tcacaataaa atatttcttt ttcaaaacta atacatataa
     1921 ccttgattat attatatcaa aacattataa ttttagatag ttcaaggagg tagaaattta
     1981 tgattgtttt gtcatgtaat aacttaaata aaagttttgg tatagattct atattagaaa
     2041 atattagttt tacagtaaat gaaggcgata aaattggtat aatcggtgtt aatggtactg
     2101 gaaaaactac tttatttaaa ataatttcag gaatttatgg atatgatagt ggagatattt
     2161 acacttctaa agattgtgaa atcggatatt tagaacaaaa taccaatttt tattcggata
     2221 atactatttt ggaagaagta ctagaagttt ttaaaaattt gatagaaatg gaatcctatt
     2281 taagagaatt ggaggttaaa atatctgaag aaagtacaaa aacaaactct ccaattatag
     2341 aaaaaatcat ggatgaatat tcacataaac tagaattatt ttcagattta aatggatatg
     2401 gatataaatc tgaagccaaa ggagttctaa agggattagg atttagtgac aatgatatgg
     2461 ataagccaat aagcatactt tctggtggtg aaaaaactag agttctttta ggaaaattac
     2521 ttcttaagaa accaactcta ttgttacttg atgaacctac taaccactta gactctgaag
     2581 caatagaatg gttagaagta tttttaaaac aatataaagg gactgtcatg ttaatatctc
     2641 atgatagata tttcttagac caatccgtaa atagaatttt tgaagttcac aataaaaagc
     2701 ttaaagtata taatggtaac tactctaaat ttgttgagtt atctaaaata gaaaaagaac
     2761 tagagcttaa gaaatttgaa gaccaacaaa aagaaataaa aaagcaagaa gaatcaattg
     2821 aaagactaaa agcttatggt agagaaaaac acctaaagcg tgcaagaagt aaagaaaaag
     2881 cattagacaa agtagatgtt ttagataaac cagaagctta taggaaaaaa gctcgtatac
     2941 aatttacgcc ttctgtccag agtggtaatg atgttcttca aattagagat gtatctatgg
     3001 gttatggaga aagaatactt tttaaagact tggatttaga tatatataga ggggaaaaag
     3061 ttgcacttat aggtgcaaat ggtgttggca aatctacatt atttaaaata ataactaatg
     3121 aactacaacc tcttagtggc aatattaaat ttggaactaa tgtacatgtt tcctacttcc
     3181 atcaggaaca aaaaactctt aatttagata acacaataat tgatgagatt tgggaaaata
     3241 atactcatct tactcaaact actttgagaa atatgttagg ggcattttta tttgtagatg
     3301 aagaagtttt taagaagatt tctacattga gtggtggaga aagagctaga gttgccatac
     3361 ttaaacttat actatcaaat gcaaacttat tgttattaga tgagcctact aatcacctag
     3421 atattgattc taaggaagtc cttgaagagg ccttaactaa ttatgatggg actatattta
     3481 caatatcaca tgatagatac tttctaaata ctgttgttga taaaatttta gttttagatg
     3541 aaaatggaat tactgaatat ctgggaaatt atgattatta tatagataaa aaaaggcaaa
     3601 tccaagagat gagtattata gaagaaaaag aagaaaaaac tagaactcaa ataaaagatg
     3661 aaaaaagaaa agagagagaa caaagagaaa tagaaaagaa aaatagaata aaaagacaaa
     3721 atatagaaaa agaaattgaa aagttagaga ttgaaattga aaaattagat atacttttat
     3781 gtcaagaaga agtatactct aatccagata aagctaaaga agttagccaa gaaaaaatta
     3841 atttagaaaa taaccttgcc tctctatatg atgagtggga agaatttatg taaaaaggag
     3901 aggattattt gcaagaacta ttttctttaa aagacgaaaa taaaagattt tataaaatac
     3961 ttctatcttt atgtatacct attatcatac agaacttaat ttctacttct gtaaatgtaa
     4021 tagatacaat tatgataagt agtttggggg aaacatctgt agcttcagtt ggagttgcaa
     4081 accaattttt ctttcttttt aatatgtcct tatctggtat cacaggaggt gctggagttt
     4141 ttatttcaca gttttatgga aagaaggatg tgagtaatat aagaaaagtc acaggtctta
     4201 cttgtgtatt ggcaattgtt ttaagttttg tatttgttat tcctgcttta ctaacaccaa
     4261 agccaattat acacatattt tcgtatgatt ctgaggtggt aaaactttgt atagattact
     4321 ttagtattgc agtatttagt tatccattaa ttgctgtaag tactgtattt agtactggtt
     4381 caagaggtgt tagaaatcct aagttaggta tgatttgtag tgcatttgca cttgtaacaa
     4441 atgttatttt aaattatgga tttatatttg gtaacttcgg tcttcctgca ttgggtgtaa
     4501 aaggtgctgc tctagctact gttattgcta gaatatgtga attaatttta atgattactt
     4561 atgtttacct ttataaaaaa gactatatat taaagtttgg tctaaaaaat ctaaaagcta
     4621 ttgataaaat ttttataaaa tctttttcat caaaaagctt tcctatattt gtaaatgact
     4681 ctgtatgggc aattggaact gtcctgtatt ctgttgcata tgcaagagct ggtacttctg
     4741 ctatagcagc tagccagata gcaaccagta caggtaattt cttcataatg acagctgtat
     4801 gtattgcatc tggtgcatct attatgcttg gaaatgaact tggagctgac catattaaaa
     4861 gagccattga atatgctaaa aagttttcta tacttgtatt ttcagctggt ttaatacttg
     4921 gaattatttt aattttaaat ataccattat tattaaaaat gtttagtgtt tctgatagct
     4981 tagcttctga tataactaag atattcttta taatgggaat actaatggct cttaaatctt
     5041 ttaatacatt ggttataatt ggtattttaa gaagtggagg agatactaaa tatgctctat
     5101 ttttagaatt aggatgtatg tggttagctt ctattcctct aacttttata gctgcattta
     5161 aaggtgcacc tatatttgta ctcgttttgc tcacttatag tgaagaagtg gttaagttta
     5221 tatttggtgt tcctagagca ttatctaaaa aatgggctat taatatagta aaggaaattg
     5281 attaataatt atactataca ttataagtat acaatatttc taaacagtct cattgaaaat
     5341 aactaaagta tttttgattt tcagtgagac tatttctatt ttttacagaa attattatac
     5401 tttattttga ttaagtaaca atttttactc atcttccaaa aattctttta cttcaaatgc
     5461 caatttatgt aattcatcat agtttaaaat ttcatatgta ttacccattt tttttactat
     5521 ctccttttca gttaacttta gcagagaatg gtatctactt tttctactaa ttgaatagat
     5581 ttcactgaaa ttacgaatag cttttatatt gcagacatta ttttcttgtt tatttgatat
     5641 acaataggca ataacttctg tgcaagaaaa tagttctctt accattctgg ttttatataa
     5701 ttcatatatt cttcttgtac attttctgtg aaatagtgca ataaaatctg gtttgagata
     5761 aaactcttca aatacatttt tttcaaatcg aaataatttt atattagtct ttgcaaatat
     5821 atcacatttc aaattatgtt catatatgta actaattttg cctataaatt cattttcagg
     5881 tacaatgtca acaagaaatt tatttcctcc tctagtagta cactccacta atgcaactcc
     5941 ctctattata aagaacactt cttgtaagtc tttatctgat gctacaaatc tttgaccctt
     6001 tttatattca acaattgata ttttgtttaa attattttta ataattggat atatacttgt
     6061 tttttctgct ttcatcaaag ttacctcctt tacaatatat tgtataaaat attttattta
     6121 tatgttttaa tttatgttat ttttaataaa agatagaatt acaactaaat taaacttgac
     6181 aaatccattc tactcacgca gaataaactt atacatatat attttcatat ataatttatt
     6241 ttaatataat tctatattta taaaattata ttatcaatag aatttattaa aataaatttt
     6301 tttgggtgta tattataatt taatcgaata gaaatgaaaa atatacatac agtttaagtt
     6361 ttaagcaaca attaggggga tttggtgaca agatttttaa ctataagaaa gaaagtgtct
     6421 taatgcaact tttagaaagt cattatagac actttctttt ttatttgata aatttaaatt
     6481 atcaaaatta gattgcttta tacaactatt ttgttggaaa ctatttatta tataaggaga
     6541 aataaatgaa aaaatcagta gctctggtaa atgatagtag aaaagattta atagactttt
     6601 tagaaaataa tttgaaatta gtttttggag attctataaa tattaataga tacttcataa
     6661 atgaaataaa tgataatgat attataaatg atgatgtgat attagtaatg tcagtagaaa
     6721 gattagacaa aattataaat aatatactag ataagaaaaa agtgattgtg gtaagaagaa
     6781 cttttaggga agataaaatt tataatctac tatctttacc acagggtaca aatgttctaa
     6841 tcgtaaatga ttctgatgaa actactctag aaactataag tttattttat aaaattggag
     6901 tgactaatat tagaccaatt ccatatatga atgataataa ctataaaaat ataaaaatag
     6961 ctataactcc aggagttcca gaaaaagtac ctagctttat ctcagatata tttgatttag
     7021 gtcacagata tatagatatc tctacattta tagaaataat aaatttatta caaattgatt
     7081 caaaggaaat acaatctaat ctagttaaat actcagaaga aataataagt ttagatactg
     7141 gtataaaaga taaatataag gaattatttt taaaaataga agaattagat acaatcttga
     7201 atttatcaaa agatggtata ttatttactt caaaagatgg tgaaataaat acatataata
     7261 gtaaagtaaa agatatttta gacataaatg aggatatata tggaaaatac attgaggata
     7321 tatttgtaga tagtttaaaa gttttattga gtgagaaaga gatactagat aaagttgtcg
     7381 tattcaataa gaaatatata aatgtaaata aaaaaaacat atacaataga gatgaaaaaa
     7441 tgggcacata ttatagttta caagaaataa catacataaa aaaattagaa caaaatctca
     7501 ctaaaaaatt aagagaaaaa ggacaaatag caaaatatac atttaaggat ataaaaacaa
     7561 atagtcctaa aatgtttgaa tgtatagatt tagctaagaa ggtttctaaa tctgatttaa
     7621 gcatacttat cagaggtgaa agtggaactg gtaaagaact catagctcag tctatacata
     7681 acaactcaaa tagaaaaaat caacccttta tagcagtaaa ttgtgcagcc gttcctgaaa
     7741 atttattaga aagccagcta ttcggatatg acaagggaac ttttactgga ggtctaaaag
     7801 atggaaaaca aggattattt gaacttgcta ataatggaac aatattctta gatgaaatag
     7861 gcgatatgcc tttagaatta caaacaaaac tacttagagt tcttcaagaa aagcagataa
     7921 tgcctgttgg atctcacaat gtaattaata tagatgttag aatcatatct gctacaaata
     7981 agaatttaga acaaatgatt gataatagcc aatttagaga ggatttatat tatcgattaa
     8041 atacaattcc aataaatata cctcctctaa gagaaagaaa agaagacata ttaattatta
     8101 tggaagattt aattaataag aaattagtaa tcacaccaga agcaaaaaaa cttatacaaa
     8161 actatatgtg gaaaggtaat ataagagaac tccaaaatgt tacatcctat ttaaacatta
     8221 tgtgtgaaga tattgtatta gagaaagatt tacctccaaa cttaaggtct tcagataaca
     8281 aaaatacatc attaaaacta aaatatagta agaatgatat attgaatata ttagagatac
     8341 taattttaaa taaggaatca gatgttggta ttggtagagg attaatttta aaagcactac
     8401 tcgataagaa ccttcaaatt acagaaggaa aaattaaaaa aatatttgaa tatctaaaaa
     8461 aagaagaact tataatttgt agttcaggaa gatatggaag taaaataact caaaaaggag
     8521 aagattttta caataaatta aaatataaag gtctataaaa ttataattta tcaaacaaag
     8581 aaatctattc aggattaaag tactccttca ataggttttt ttatttttca agtaccctaa
     8641 aattataatt tattttgtac ataaataata ggaattaata ggaataggat aattttttta
     8701 tttcctattt tttttatata aactagcata taaaatatat tttcttcaaa ataatatttt
     8761 ttactgaaaa ttgaaactat gcaatcattt tttatactat acatatagat aaatgattat
     8821 atttattcaa tattcttata aaaaacaaaa ttctccatcg aaaattatgg cataataatt
     8881 gcttattata ttaataaaac aataaaaagc aattggagga ataaaaatgc aacaggcttt
     8941 gaaattaaaa tatcaaacca aagaattagg aaaagatttt gtaatagaaa gttgtaattc
     9001 tataaaacca tggcttataa acataagaag agaattacat aaaattccag aattagctct
     9061 tgaagaaaat cttactaaac agaaggtaat ttcttattta aaagaaatcg gaattgatta
     9121 tatggaattt acaaaacaca atggaattat ggcatatatt ttaaaagaaa gtgcagataa
     9181 aacgatttgt ataagagcag atatggatgc tcttcctatt gaagaagaaa acaacatacc
     9241 ttataaatca atacattctg gaaaaatgca tgcttgtggc catgatgcac atacaaccat
     9301 gctacttgga gcatgtaaag ttttacattc aataaaagat aaacttaatg taaatgtaaa
     9361 attcttattt caaccagcag aagaaggttt tggaggagca aaatttttag ttgaggatgg
     9421 gtgtttagaa aaccccaagg ccgattatat attcggactt catgttatgc cacatataga
     9481 aacaggcctt atcgaaacaa aatatgatac attaaatgct agtgtagaca ctataaaaat
     9541 aagtatcaaa ggtaaaagag ctcatggtgc atatcctgaa aacggtattg atgcaatagt
     9601 tacagcttca caaattgtta catcattaca aactataata agtagaaact tagaaccaaa
     9661 taatgctgtt gttttgacta taggtaaaat atatggtggt gatgcacaca atgtaatatg
     9721 tgaagatgta aaattagaag gcactttaag aaccttaaat agcaaaacaa gaaactttat
     9781 gattgataaa atagctaaaa ttgttggaga tactgcatct gcatttggtt gtgttgggac
     9841 tttacatgta agtgatgaaa actatcctgc tgttataaat gaaaaagaat tagtagatac
     9901 agttatatca aatactaaag aattacttgg agaggaaaaa tttatattga gaccaaaccc
     9961 gtctttaggt ggtgaagact tttcttttta cacagaacat tgtaaaggag cattcttcca
    10021 tttaggatgt aaaaatgagg aaaagggctt aatttcacct cttcatacct ctagctttaa
    10081 tatagatgaa gattgtttac caataggtgt tatgatgcat gtaatgaata ctttgtattt
    10141 taattagtac agtaattaat aaaagaatag gagataaatt aatatggaaa aagctaagaa
    10201 attaggtcta tttagtatga tattattagg gattaatagc ataataggtt caggtatatt
    10261 cttactacca ggtaaagttt ataatctagc aggtcaaaac agtatgttta tatacatttt
    10321 tgctacactc ttagttctat caatactttt atgttttgca gaagttggaa gcatgtttga
    10381 taagaaagga ggagcatatc tatactccaa aaaagctttt ggagatttta taggatttga
    10441 agttggtaca atgtcttggg ttattagaat tatatcatgg tctactctag cagtaggatt
    10501 tgcaacagct ttaggttcat tttggccaga atctgccact gaatacaaag gttatatagc
    10561 tgcaatactc gtaacactac tttcaataaa tagcttattt ggaattaaaa gcacaaagat
    10621 aatgaataat gtaataacta tagctaaatt agtaccatta atagttttta taatagttgg
    10681 aatatttttt ataaaatttg taaatattgt tccttcagga aatgtagtta actctagtat
    10741 gggtccagcc attatattag tattttacgc atttaccggc tttgaatcat ttattgtggc
    10801 aagtggagaa atggaaaatc ctaaaaaaaa tcttccagtc gcattaataa ccacaatatt
    10861 tatatgtgca ataatttata tattaattca aattgtctgt atgggtatac ttggagatag
    10921 actatttgaa aacagtattc caattgcaga tgcttcaagt gtatttgtag gcaattatgg
    10981 taaagttttc atatctgtag caacattaat atctatattt ggtataaata taggttcatc
    11041 aatagtaact cctaaatgtg gttcttctct agcagaagaa ggttctcttc ctgcatttat
    11101 aggaaaaaca aataaatatg gtgctccata tgtagcaata ataatatctt taatttgttg
    11161 tataccactt gtgctaactg gaagttttga acaacttgca gtaatgagcg taattgcaag
    11221 atttgctcag tacataccaa cttgtttatc agtaatagtt ttaagaaaaa gaacagatgt
    11281 taaggcctct tttaagattc catttggccc tgttatacca atagtagcta ttttaggtag
    11341 cttatggttg ttacaacaag cttgggcaga agatatttca aaacctataa ctcaaaatag
    11401 agttttaata ggattagggg caatgttgtt aatagctcct ctatatatat ttatgaaaaa
    11461 aaataaggaa atcgagcata aaaataaatc agaagattct aaattatata tttcgagaga
    11521 aaaaattcaa taatatttta tactattact tctcataaaa tataataaat ataaaaaaat
    11581 tgtagataaa actatttt
//
LOCUS       DAMJVI010000109        11152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.114, whole genome shotgun sequence.
ACCESSION   DAMJVI010000109 DAMJVI010000000
VERSION     DAMJVI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.114"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..632)
                     /locus_tag="PC545_003044"
     CDS             complement(<1..632)
                     /locus_tag="PC545_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2457790.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
     gene            complement(704..1057)
                     /locus_tag="PC545_003045"
     CDS             complement(704..1057)
                     /locus_tag="PC545_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2457791.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            complement(1135..1338)
                     /locus_tag="PC545_003046"
     CDS             complement(1135..1338)
                     /locus_tag="PC545_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2457792.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            complement(1728..1958)
                     /locus_tag="PC545_003047"
     CDS             complement(1728..1958)
                     /locus_tag="PC545_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457793.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            complement(1960..2367)
                     /locus_tag="PC545_003048"
     CDS             complement(1960..2367)
                     /locus_tag="PC545_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2457794.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            complement(2727..3176)
                     /locus_tag="PC545_003049"
     CDS             complement(2727..3176)
                     /locus_tag="PC545_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457795.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            complement(3190..4116)
                     /locus_tag="PC545_003050"
     CDS             complement(3190..4116)
                     /locus_tag="PC545_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457796.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            4344..4571
                     /locus_tag="PC545_003051"
     CDS             4344..4571
                     /locus_tag="PC545_003051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457797.1"
                     /translation="MIVACNALTQLLYSWLATVFSNASIYWGASNSIFLTPRGNLPKR
                     SCINGASTDCLKKDKKSIVSNSSLRPILVQA"
     gene            complement(4795..5415)
                     /locus_tag="PC545_003052"
     CDS             complement(4795..5415)
                     /locus_tag="PC545_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2457798.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNVKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     RGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLRCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(5514..6782)
                     /locus_tag="PC545_003053"
                     /pseudo
     CDS             complement(5514..6782)
                     /locus_tag="PC545_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            6874..7679
                     /locus_tag="PC545_003054"
                     /pseudo
     CDS             6874..7679
                     /locus_tag="PC545_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            8351..9118
                     /locus_tag="PC545_003055"
     CDS             8351..9118
                     /locus_tag="PC545_003055"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDF2457799.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKKDIIKEAKELKDACLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYIHDIPLLFTFRSVVEGGEKLISRDYYTTLN
                     KEISNTGLVDLIDVELFMGDEVIDEVVNFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQNEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELNSVLNLLHKSIN"
     gene            complement(9446..10123)
                     /locus_tag="PC545_003056"
     CDS             complement(9446..10123)
                     /locus_tag="PC545_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457800.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFRYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSFGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKFKRRKKRNYVCCYCPDMYRDIKLDKESINKIYNRKIKIEREVNIF
                     EDEDVIINKKVLKFPKSWTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVII
                     LYYIYNH"
     gene            complement(10273..10590)
                     /locus_tag="PC545_003057"
     CDS             complement(10273..10590)
                     /locus_tag="PC545_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457801.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVNILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLISQIIDIIKK
                     NKI"
     gene            complement(10612..10950)
                     /locus_tag="PC545_003058"
     CDS             complement(10612..10950)
                     /locus_tag="PC545_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457802.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
BASE COUNT         3679 a         1780 c         1611 g         4082 t
ORIGIN      
        1 taaactaatt tatcaatatt tcctgattga atatgcttaa tgtttgattg aattttttta
       61 tatggccaat cccaccattt aatttctaat aattttgaaa ttgtatcatt attaaatctc
      121 ttttttataa tttttgcagg tactcctcca acaatagaat acggtggaac gtcattagta
      181 actactgctc ttgttccaat aattgcacca tcaccaatat ttacacctga cataattata
      241 gaatcataac ctatccacac atcatttcca attataatgt tacccttatt gtcccaagcg
      301 tccgttatat ggttgatatc gagtttccat tcttcataaa agatgggaaa agtatatgtg
      361 gattttgatt tcattgagtg atttccacta gtcattagaa attttgcatt acatgctatt
      421 gagcaaaact taccaattat taatttatca ttgtttatag ggtaatgata caatacattg
      481 tttttctcaa aatctcttgg gtcattataa aagtcattat atattgtgta atctccaact
      541 tctatatttt cccttgttat tacattttta agatatacag tttgataatc attacttctt
      601 ggatatacct tatttgaact tggaatagtc atatttaaat ctctccttta atatatatta
      661 tgtttttgac tattccacta ctacaataca tctcttcaca gaatcactcc ttgttaagaa
      721 tcttcataag ataatggcgt ccttttttag tgttaagtcg gatattcttt cttagtccat
      781 tctttttagc gtataactga caaactgtca ttttcttacc aatagtgtca atgctatcat
      841 caaggtcatt ttgtattgct ttttccttat cccttaatga tatatcatca cgttttccct
      901 ttgggggttt atcagtaggt acaagtttcc aagagtaaac aaattgtggt ttaccaaatg
      961 aatatacata cttatatgcg tatcttccat cttttctctg gctctctcca gttcttaaaa
     1021 ttctatcttt attatctcgt cttttttcag ttgacatgat aagaagctcc tctcaataac
     1081 cgaattgagc ttgatacgtc atatgaatat tataacatat aaggctcaat ttcattagat
     1141 aaagtcaagt gtatcaacga ttttctcaaa ttgttttctc tttatttgaa tgcgattgct
     1201 attcataatg acccagccag aatcaaggtt ttcatcagca agtttacgta attttttctc
     1261 tccaatacga aagtattttg acgcttcttc aatcgtcaag gtatattttt cccaaatagg
     1321 tacatcagta ttgttcatag aatgtatcac tcctttctat gttcgtagtt cagaagtaga
     1381 tagatggctt tggtaagctc cattggtatt ttaaccaatc ccattcttat gggcagaacc
     1441 gtgtcataca ggtgtatcat tatactgtgg tacaggttac ggcaaaacaa ccgttccaaa
     1501 ggttattcac aaatcactat atagtagctc tcttgattaa ggtcatggcg tatagtattt
     1561 gttggctatc tgtatcacaa acgtatctat ctactttatt cagttgtcaa agaacagtcg
     1621 gcattgattt gtctataaaa aatcacaata gcttatgggc ttttatagac aaataattag
     1681 tgattctcca tgtaccaaat agcttgatac atggagagag ggcgagatta agacaaatta
     1741 aatctcaaaa tagcagtgat tagttttgtt cctagcattc gctttgtttc ttcttctaca
     1801 tgaatatagg gatttccgta ttcatcaaat aaagttttct gtgaaagacg aatgatatag
     1861 tgttcaaaat gtttaagtat tccgtttatt gcatttacat cgccatgtac agcaagtaaa
     1921 ataattgtaa aaggtaatac ttctttagta ggtttcattt caatgttcct ccatgagttc
     1981 tttcaactct tttagtgcat ttgttttgtg ataatgtatg gtattttctg ctaaatccat
     2041 caatgttgct atgtcagcat ttgtcatgtt gagaaagaat gaaagtagaa ttatatcttg
     2101 ttttcttttt gtaagttttc cgattgcttg tgccattaat acatcttcaa ttcgtatgtt
     2161 gtagtcgtaa gaaaccatac agatgtagtt agaatcataa tcatctaaaa tactcaattt
     2221 acttaattct gcaagtgaca aagattcgag agataccaaa taatcatttc tgcgtttatt
     2281 ttcgtcataa atatctctag catagttacg caaaactgtt ttacagaaac tgtcgaattg
     2341 atgttcaatt gtcttttcat caaccataag atttcaccct ctttccatcg caaggagtta
     2401 aataaccttg cttactccta tcacggtggg agggcgttaa atcttcgata aatttcaaaa
     2461 aaaagagaaa aaaatacaca ctctgcctat agggcaaaaa tgtgtattct ttaaaacgta
     2521 tttaatttat attggttgta agccaccacc atattgatgt atggaaatat gcattggcta
     2581 gcttttcata aaaatacaaa gatgcataag aaattaatag aggggaaaaa cttattctgt
     2641 tattaaaaag taggttaatt ctaataaatg ctattgcggt cgaattaaac tatatacatg
     2701 gcaatcaatc atgtttctat tctttattat gaattgtgac agtattcttc aatcttaaaa
     2761 ccacattttt ccaatacttt acttgaccct ctattgtagg aatatacatt agcatgaatt
     2821 ttgttaaggt tgtatttttc aaaggcaatt ttgcatattt cttttactgc atttgttgct
     2881 atccctttgc cccaatagtt cctagcaagc cagtaaccta attcagcatt tttattaata
     2941 gaggtatctt cttcaaatgt taggctgata cttccaatag ccctttcttg ataaaaaata
     3001 gcatagctaa gttgtttaac atctggagtt gccaaacaaa aactaacaaa atctctagcg
     3061 ttttgctccc tataagggtg ggggaagttg tcacgaagat tctttgcaat ttctttatca
     3121 ttcgcaaact caactaaatc ggtaatataa gcataattcc actctataag ttccattttt
     3181 cttctccttt tatgaaacag ctacaagaaa tctttctaag ccaataccac ttatcaaaca
     3241 gcgttctttt ttatttccaa gcatttgata agtccagtca acaaatccac cgtcaactat
     3301 ttcaacggtt tcatttcctt gtttgaccga taatttgaaa ttgatacctt tataataatt
     3361 attatcaaca tcatcattat gaattgataa ctctatatta ggaaaaataa gtttgatagt
     3421 ttcaaccatt ctgtcgaaaa atccgtcatt atctttgtat ccgtttcttt tgcgtagaat
     3481 aatagagaaa cgaacatttt ctatttcact taatatatct ttataatagt ttaagtgctt
     3541 ttctaacaac attttttcac aagtgtaaga ccctgtatct ataccagatg aaaccataca
     3601 atataagcca aaatgtgaat ataaaccttt acctacaaaa ttttgtgctc ttattaccct
     3661 agcagtagta gccatgtgga taggtatggt attattttct tcttttcttt ttaatttatc
     3721 agcaataatt attgccaaca tatttgaggg gtctgcaagt gtttctgtcc ctcgtagagc
     3781 acttactaca ttatattgat ctacggagcc aaaaacgcta cagctaccta atggagcaga
     3841 gggagaaagc ataatcgtct tgatatccat attgaatgct tttctcaata attttgactc
     3901 caaagcatga atttcttctg gatctatacc gctagggata gtaaatctat ttagttggta
     3961 tgactttaga atatcagttg cagttaaagt atttgcttgt ctatcaaaca cttctaatag
     4021 aagtgagttc aaatctgatt ttgatagagc tgataactta tcaactatat tcttatcccc
     4081 aattttattt gtaattctac ttaaaatatc actcatagtt atcctccata ctgttacttt
     4141 caaaataatt atagaatggt tacaacaact aatctaggtc atttgaccaa aaatgaaaaa
     4201 acactgaata tttactattc aatatttttt tactttgggt ttatttgatt aaatcttgaa
     4261 aatactgtaa tgcaaccctt tcagcttcaa cctttcggac aataggactt tgtgataatt
     4321 cctctcttaa ctcaagatac aggttgatag tagcctgcaa tgcattaaca cagcttttat
     4381 attcatggtt agcaaccgtc ttttctaatg cttcaatata ttggggtgcg tccaattcaa
     4441 tttttctaac tccacgaggt aatttaccta agagaagctg tattaatggg gcaagtactg
     4501 attgtcttaa aaaagataaa aaatcaattg tttcaaatag ttctctacgt ccaatcttag
     4561 tgcaagcgta atgaacccaa atccaaaatc tatcttcaat ccactgtaag tctggagttg
     4621 ggaagtatgc ttctttttta gtataacacc tctcactttc ttttattagc tatcaatcca
     4681 acataattat gcataggagt attatataat gaaaaaataa aaaaaaatag gtcacttgac
     4741 ccgaaataaa aaagcattga ataattatta ttcaatactt ttttgaaatt atacttatgt
     4801 tgtaggaaga ttctttaata cctctcgctg attaattaca ctaaatcttt tgttttcata
     4861 agcaattaga tgttcttgta cacattcatt tatagcacga ttaatgcttc taattggagc
     4921 gtttacttcg ctagcaagct gttttttggt aagcagattt aattcacttc tataatagtg
     4981 taaggatata cacctcagaa gtcgttcttt aacacttaaa gatgataatt cttcaattaa
     5041 ttcagcattt acaactaatt tactagatat ttctcgaatc agaaattttg tagcttcaaa
     5101 atctcccctt aaccagttta aaaaatcatc tctatgaagt ttatatacat tacaatctgt
     5161 gaaagcgaca atttcaacgg gttttttccc atcattaaat tgttccattt ctccaaaaaa
     5221 actaccactt ctataaagat gaatagttga gatgtttcct ttcacattta agatgaatgc
     5281 ttctgcaata ccgtcaatca taagaattac atattcattt tcttgctcag caaaaagaat
     5341 agtctctttc tttttatacg tttttttaac aagcttcttt ttaatatctt cattcataaa
     5401 atcaattaaa tgcatattca tccctcctta ccaaatcttt gattaaatta tatcatcttt
     5461 caattagtta ggtaagatag taaaaatata tttacataga aactattcca ttattatttt
     5521 ccagttgtca tttttctgca aagtaagctc aaactgagaa agctgttgac aatagaacta
     5581 gtaggaatat atgagcgaac catttcagta ttcaactgtt gtaggttttc agtcagataa
     5641 ttcttcaaac gttcaattcg tttgtcctgt gaatcttgcg atgctctaac cagtttcata
     5701 gatagcgttt taccttgtgc cacctgcaaa aaagaccatg cttcgtcaag gtcaactatc
     5761 ttgaagctag agcggtcagt atgaataaag tctaatgcaa aggtactaat aacaatcaac
     5821 atagcaacac taagaagttc tattgttgta tattcttcaa agctagtgtt ggcatctggc
     5881 aatacgaggt cagcaacttg aataatattc agctgtttct ccaagctgat agactgcttc
     5941 acatttccgt caccaaacag tagatgtgca aaatcatagt ctacaaaact ttcgatatgg
     6001 tcggctatgc tgttactgat agaagtatct tcttttcgta attcatcaat gacaagtaat
     6061 aaccctcgct gttcactttg ggttacattg cggattgctt ttctaagcac tgggaactta
     6121 tcactgtcac gactggagat accagcaagg aatgttagga tatcaatcgc taaactttca
     6181 gaatcatttt tacggctcaa aatgacgtaa gggtcaagca atcctttgtt ttcatcatta
     6241 ctggttaaat tgacaatgtt tatctcatgg gctatttctg gtagcgtttc tttccatttg
     6301 ccgcgttctg ctttagggtc aactatgacc gcctgtccgc cgaatagtac cgcatagtaa
     6361 acaagcatat tgttactgaa taatttacca ccacccaatg aaccgataaa cgcagacgct
     6421 aacacattgg taatagaacc ctttacccat tgactagcaa gggcaggttt tagatagaca
     6481 ttctttccag tgtccagatt gtagccaaga tagatatcct catgttcgcc aagggtttgt
     6541 gtcgctccaa aaccaagtcc agccaaaaaa tccgaagtga catattgaat ataatcattg
     6601 atgtaacgtt tgctgacagg aataaattcg ccatgcaatc ccaacatatc cccaaaggga
     6661 cgcacgagct tgagtgataa gtcgtcgtaa aagtctttga cttcatcaca acgccttttt
     6721 agctcgtcaa gatcctttgt agatacacgg acaacatagc taagtttata catagattct
     6781 ttacagttaa aagtctgttt ttaaaaaaat gtataaatta taaatcaaaa tatagatact
     6841 atcaatgacc cactaggtgc aaaaagcatt gatatggtta aattatcaga cgctaaaagc
     6901 tgggctatcc gaatgggtgt gcttataaat ccattgacaa ttataagcgc tcactgaaag
     6961 cgtcgttttt catggcaatt caagacgact gtattagaaa gaatccattt aattttcagt
     7021 taagtgatgt tttggaagat acaacagaac caaaaatcgc tctaacacca gaacaggaaa
     7081 tgggcttatt atcctttatg gagagcgata agacttattg taaatattat gatgatgttg
     7141 ttatcctctt agaaacagga ctacgcattt ctgaattatg cggtttgact ttagaattgg
     7201 atatgaaaaa taaagccatt atggtagacc atcaattgtt gagtgatact aagattggct
     7261 attatgtaga agttccaaag accacacagg gtaagcgaga aattccaatg actgaaagag
     7321 cttatcaatc atttgaacgt atcgtggaac ggcgtaagaa cgctaaagca gaaccaatca
     7381 ttgttgatgg ttaccagaac ttcctatttc ttaaacaaga tggattacca aaacaagcaa
     7441 ctcattatca aagcatgtta agagcgttag tcaagaagta taataagacc catgaggaca
     7501 agctaccaaa gattacgcca cataccttta ggcatacact ctgtacaaat atggctaaca
     7561 aaggaatgag tcctaatata ttgcaatata ttatggggca taaaaacatt actatgacgc
     7621 ttggctatta tgcacatggt tcatttagtt cttcgagagc tgaaatggaa cggttataat
     7681 cctagtagta tgactagagt ttactactta tttactactt ttgatggcat tcttatacgg
     7741 ttaaatgcca acttatgcaa agtatcttcc aaaaagaaaa tgccgatact ccttgtagat
     7801 acaggatata tcgacattta ccaacttatg aaaagataat caaatttaat ataagttttt
     7861 caaataaaaa gataccaaat tctttttcga gaatctatat aacattactt aagcttggtt
     7921 ggggtataaa caattgagta gctacttttg tatggtgttc aactttagtc aaaactctta
     7981 agtaatgcaa atgatttaga ttcattaata ttcaatctct tcataagtag tgaaattcga
     8041 gttatttctg cataattata ctatgattta tagattaatt ctattagcat ccagaataat
     8101 attgtttata tgagtaaaat ttaaagaata ttgtgaaaat tataggatgt taaaataaat
     8161 ttaggtaaaa aagaagtgtt tgtatattat agagtacctt ttctaaaata tgtgggattg
     8221 ttttaagaat tagaataaaa attattttta aattagtatt tttataatgt atagtatttt
     8281 taatgtataa ttaaattgtg cagaaaatag aatttaaagt cctatagaat ttctgattat
     8341 ggaggagaat atgaaaagaa aagttcaagt aaaaaatatt actattggag aaggtagacc
     8401 taaaatttgt gtaccaatta ttgggaaaaa taaaaaagat attataaaag aggctaaaga
     8461 gttaaaagat gcatgtttgg atattataga gtggcgagtt gatttttttg agaatgtaga
     8521 aaatattaaa gaagttaagg aagttctcta tgaattaaga agttatatac atgatatccc
     8581 tttattattc acatttagaa gtgtagtaga agggggagaa aaattaatat caagagatta
     8641 ttatactact ttgaataaag aaatttctaa tacaggttta gtagatttaa tagatgtaga
     8701 gttatttatg ggagatgagg tcatagatga agtagttaat tttgctcata aaaaagaagt
     8761 gaaagtaatt atatctaatc atgattttaa taaaactcca aaaaaagaag agattgtatc
     8821 tcgtttatgt agaatgcagg aattgggcgc agatttaccc aaaatagctg ttatgccgca
     8881 aaatgaaaaa gatgtgttag tgctacttga agctacaaat gaaatgttta aaatttatgc
     8941 agatagacca ataataacaa tgtcaatgtc aggaatgggt gttataagta gattgtgtgg
     9001 tgaaatattt ggttctgcat tgacttttgg agctgcaaaa agtgtttcag caccagggca
     9061 aatatcattt aaagagttaa attcagtact taatctatta cataagagta ttaattaaat
     9121 taattatata gaaatattag acggtatttt tgaagatata aattaaatat aaaaaacttt
     9181 tcagatagta aaatttaaat tgggcaatta tatctgaaaa gcaactattt agtgtgtatt
     9241 ttgaaagtga tatagaaaat cttataaatc aactatggca tatttaaaat atgccattaa
     9301 aacctactag acattagatt atagatggta tactagcatt tctattattt atagatagta
     9361 agttttcata ataactgttt tatctaaagt agaagatata tttgtattat gtatatttgt
     9421 cattattttt ttgtaattat gtatattaat ggttatatat gtaatataat attatcactc
     9481 tattttttgt atattgtaca ttacaacatt tatatctatc atcatcaatt acagtagaat
     9541 gtatgggata cttattttca cttaaccaat atttttgcat attctttgtc caagactttg
     9601 gaaacttcaa tactttcttg tttattataa catcctcatc ttcaaaaata ttaacttctc
     9661 tttctatttt tatttttctg ttgtatatct tattgataga ttctttgtcg agttttatgt
     9721 ctctatacat atcagggcaa tagcaacaaa cataatttct tttttttcga cgtttaaatt
     9781 tcacaacttt ctctatgcta atttctaatt tttttgtacc atcttttata aatttaatat
     9841 cttctatgtt ctcgcttgaa aaaatttctg tcaatcttaa agtatctaca gtttctctaa
     9901 ttatttgctt tttataagaa gagaatccat atccaaaaga aaggtttaat ttattaggga
     9961 tgtagccatt tttatattta aacatcttaa ttttttccaa ttcagcatta tctttaaatt
    10021 tcaacttttc atacctaaaa gttactttgc taaaaagagt atattctaca tcaatatatt
    10081 cttttgcaat cttaaattta tcgctacctt caatcacaat catagcctat ctccaatcgt
    10141 tttatattaa aataaggcca tagaacatgg tctaatagta ctcattagca ctataagaca
    10201 atgttaacac aaaaagacaa aaaaaggtta tttatttcaa ataacctttt tttttatata
    10261 aaatataaag aattatattt tattcttttt tatgatgtct ataatctgtg atatcagatt
    10321 tagttctctt gaatttaagt cttttaactc ttcatttata tcattttcta aatatgtttt
    10381 tccagtggaa gtaatatctt ttataagaac atctgcagaa atatttaata tatttacaaa
    10441 ttttatgaat gttttaagac ttggattctt tttaccactt tcgatttgag ttatatacca
    10501 aggcgatact ccaaccatat ttgcaaattc ttttgaagtt aaatttgctt ttttccgttc
    10561 ttctttaagt cttttaccta tataattcat tattgttact atttccctca cttatatttc
    10621 tttgtattta atgatgcact ttattaactc tagcattgca atttgttctt tttcagttaa
    10681 tttattaatc atttcataac attctaaagg tacagagtta tcaacgcctt ctgttgtaat
    10741 aatatttgca aataacaatt caagtggtat gtctaaagca gtagcaattt tcattataat
    10801 attgatagat gtatttgtct cagctctttc aatctgacca atatatgttg gatgtaaacc
    10861 tgccttttct gctagaaact cttgactata tcccaatttt gttctataga cttttattct
    10921 atctcctatt atttttccta tatttgccaa ttattatccc tccactttag attatattca
    10981 tagtttaagt tgttttatat agactattta tactttttta gtctaaatat agactatata
    11041 tgtttttata ctgaaataag tgctatttat ttttgtatag tctgtatcta caataaacaa
    11101 atatggataa atcatatttt taattagaga aatattttca taatttcata ac
//
LOCUS       DAMJVI010000110        11101 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.115, whole genome shotgun sequence.
ACCESSION   DAMJVI010000110 DAMJVI010000000
VERSION     DAMJVI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11101
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.115"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            258..704
                     /locus_tag="PC545_003059"
     CDS             258..704
                     /locus_tag="PC545_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457803.1"
                     /translation="MAISDLAFGTECLKTSFQMLFSVISLVNINLLQAQILIAIGGLI
                     SILAMVSLTLFLSAKCKDSLTSMLIAFSICLIPMGIGVSWIGYLLPPGGIGFKISLLY
                     QMTSFNFLHLGGMSFWTPHIILFFSVIEIPIFIFLAIRTYCKHQVP"
     gene            1085..2002
                     /gene="pstC"
                     /locus_tag="PC545_003060"
     CDS             1085..2002
                     /gene="pstC"
                     /locus_tag="PC545_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDF2457804.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKVAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGADWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPTIQGIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            2004..2828
                     /gene="pstA"
                     /locus_tag="PC545_003061"
     CDS             2004..2828
                     /gene="pstA"
                     /locus_tag="PC545_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDF2457805.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGNMNFNFL
                     FSNYSASGDGGILPMIITTLYTVVVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSVGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            2847..3611
                     /gene="pstB"
                     /locus_tag="PC545_003062"
     CDS             2847..3611
                     /gene="pstB"
                     /locus_tag="PC545_003062"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDF2457806.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGNISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRTHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTMFTTPVDKRTEDYITGRFG"
     gene            3639..4292
                     /gene="phoU"
                     /locus_tag="PC545_003063"
     CDS             3639..4292
                     /gene="phoU"
                     /locus_tag="PC545_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDF2457807.1"
                     /translation="MVNNSLDISIDTLKEQTVLMMEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEINKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIEGKMVEIG"
     gene            4450..5319
                     /locus_tag="PC545_003064"
     CDS             4450..5319
                     /locus_tag="PC545_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2457808.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKILRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            5406..6842
                     /locus_tag="PC545_003065"
     CDS             5406..6842
                     /locus_tag="PC545_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HDF2457809.1"
                     /translation="MNTRNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDNLNNYFSGKFDKDTMVNLIRVTMGLDVTEDTDKNLKESLHEAVNLDEVKRPIIS
                     VISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFVGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIEATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNKKENKYKK"
     gene            6890..7717
                     /locus_tag="PC545_003066"
     CDS             6890..7717
                     /locus_tag="PC545_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2457810.1"
                     /translation="MKIKDNKAVFHVFIRIFLIAVLILTGTLLEEKINNVPVENISSK
                     GENTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYNKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENITPKNFWQENL"
     misc_binding    8013..8275
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8351..11017
                     /locus_tag="PC545_003067"
     CDS             8351..11017
                     /locus_tag="PC545_003067"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDF2457811.1"
                     /translation="MENTNLSKTYNPKDFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPDKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDANAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
BASE COUNT         4388 a         1192 c         2080 g         3441 t
ORIGIN      
        1 taaaatagtt aaataataag attaagaatt tcttaataaa tccagaagat gctttcgatt
       61 atataatatt ttatattttt atattattaa ttatatgtgt tgcaatcgct gcacctactt
      121 tttctggtga gtatcagaca caatcagata gcatccttcg ttgtacgaaa catggacata
      181 ttcgtctagc gtttactaaa atattagctc tattttctat atttataata atgtttgtta
      241 tatgtatttc tatccatttg gcaatttctg atttagcttt tggtactgaa tgtttgaaaa
      301 cctcatttca aatgttgttt tctgtcatta gtctagtaaa tataaattta ctacaagcac
      361 agattcttat agctattggt ggtctaattt caatacttgc aatggttagt ttaacattat
      421 ttttatctgc aaaatgtaag gattctctta cttctatgct gattgctttt tctatttgct
      481 taattcctat gggaataggt gttagttgga ttggatacct attaccacca gggggaattg
      541 gttttaagat tagtttatta tatcaaatga catcatttaa tttcttacac ttaggaggta
      601 tgagtttttg gacaccacac attatactgt ttttctcagt gatagaaata cctattttta
      661 tatttttggc tattcgtacc tattgcaaac atcaagtacc gtaacaattt gatggaaata
      721 atagtgatat aaactagagg atacaataat tatatatgta gtataaaagt taaatttagt
      781 taacaaatct taatagcaga tataggtaat aaattataca taaactgtat tattatctat
      841 atctgctatt ttaatataga gaaacaacag tatattgtaa agtaaatgtc aaatatagtt
      901 ttaaaagtaa aattaatgtt aaaaagtaaa tataatttta cacttgctta acttaatcct
      961 aatatagatt taacttaaaa aaagtagaat aaaagtatag gaaatttgat gaagacgaat
     1021 tataaaaaat taaattttga atacagaacg aaaatcgata agtatatttt aatgggaggg
     1081 tattatgata gtaaaatcta gattagtagg tttagaaaaa aatgacaata atggcaataa
     1141 gacaaaatac gtaattgaaa aagtagcaaa aaatattttt ctacttagtg ctttagtagc
     1201 agttgcaagt ttacttttga taataggatt cgtattttat aaaggattaa gaccatttat
     1261 atttgaagga tattcattta tagacttttt aactggtgca gattgggttc cttctgccaa
     1321 taaatttgga atatcatcta tgatagtagc atcaatattt gcaacacttg gagcacttgt
     1381 tataggagta cctgttggta tattaacctc tgtatttata gcagaagtag cacctaaaag
     1441 attagcaaaa ataatgtcac cagcagttga acttttagca ggtatacctt cagtattata
     1501 tggtgtattt ggtcttgcag tgatagttcc aactatacaa ggtatattta atcttccaaa
     1561 aggtcaaagt ttgttagcaa tagtaatagt attagcagta atgatgttac caacaataat
     1621 atctgtatca gaaacagcaa taagagcagt accaaaagca tacaaagaag gttcattagc
     1681 acttggtgca tcaaaaatag aaacaatatt caaagttgta ttacctgctg caaaatctgg
     1741 agtattagcc gcagtagtgt taggagtagg aagagcttta ggtgaaacaa tggcagtaat
     1801 acttgttgca ggcaactcac cagttatgcc aactgcaata acagatagtg taagaccact
     1861 tacaactaat atagcacttg aaatgggata cgcatttggt acacatcaag aaatgctatt
     1921 tgcaactgga gtagtattgt ttacatttat attaatttta aacttagttt taaataaact
     1981 ttcaaataag gcggtaaatt aagatgagaa agttcaaaga gaatctattg aagtcattag
     2041 tatatctatc agcactattt acaatagtat cattagtagt tatagtggga tttatattta
     2101 taaagggaat tggcaacatg aattttaatt tcttatttag caattattca gcatcaggtg
     2161 atggtggtat acttccaatg ataattacaa cattatatac agtagtagtt tctatagttg
     2221 tagcaactcc tataggaata ttggctgcta tatatttaca agaatatgct aaaaaaggaa
     2281 aagcagttac agctataaga tttgcaactg aaagtttatc agggatacca tctatagtat
     2341 atggattatt tggaggaatt ttctttgtag ttacattaaa aatgggatat tccatagttg
     2401 caggttcact tacagttgca ataataatat taccagtaat aattcgtaca acagaagaag
     2461 ctttaaaaac agttcctcaa gcttatagag aatcatcatt ggcattagga gctacaaaat
     2521 ttcaaacttt atataaggta gtacttccaa gtgcaatacc aggaatttta tctggagtaa
     2581 tcttatcagt tggtcgtata attggagaat cagctgcaat attacttaca gcaggtactg
     2641 tagccaagat gccaggtgga atatttgata gtgcaagaac attaactgta cactcatact
     2701 tactgactaa agagagtgga gatatagcaa cagcagccag tataggaatt gttttaatag
     2761 taattgtatt agcactaaac atgctagcaa gatttgttgc taagaaatta aataaagcta
     2821 attattaaat ttaagaggtg tgaataatgg aattaatcga taagataaaa atgagtgtaa
     2881 aagatttgga tttattttat ggtgataagc aagcattaaa gaaaataaat atggatataa
     2941 aagagaataa ggttactgct ttaataggtc catctggatg cggaaaatca acttttataa
     3001 gaacgttaaa tagaatgaat gacttaatcg aagatgtaac aattaaagga aacatttctg
     3061 ttgatggaga ggacatatat acctcagatg atgttataaa tcttcgtact aaagtaggaa
     3121 tggtatttca aaagccaaat ccatttccta tgagtatata tgataatgtg gcatatggtc
     3181 ctcgtacaca tggactaaga gataagaagc aattagataa aattgtagaa gaaagcttaa
     3241 aaggtgcagc tatttgggat gaggttaaag acaggcttaa atcatctgca ctaggacttt
     3301 ctggtggtca acaacagcgt atatgtatag caagagcaat tgctatgaga ccagaagtta
     3361 ttcttatgga tgaacctaca tctgctttag acccaatttc aacattaaaa gtagaggaac
     3421 ttatagagga tttaaaaaaa gactacacaa tagtcatagt tacacataat atgcaacaag
     3481 ctgctcgtat atcagatgaa acagcttttt tcttaaatgg agaggttata gaatttagcg
     3541 atacaaaaac tatgtttacc actcctgtag ataaaagaac agaagactat ataacaggta
     3601 gatttggtta aaatatataa ctataaagga gttagacaat ggtcaataat agtttagata
     3661 taagtattga tactttaaaa gaacaaacag tgcttatgat ggaaaaatgt gaagaagcaa
     3721 tagaaaagtc tgtgacttgt atgatagaga aggatttaga gggtgcaaga aaagtagtag
     3781 aagatgatga agaaataaat aaattaagag aagaaataag agataagagt atagagctaa
     3841 tagtattaaa gcaaccaatg gcaagggact taagatttat ttattcatta ggtaatatat
     3901 ctatagagct ggaaagaata ggagattatg cagcaaatat agctagagaa atgttgaaga
     3961 taggtggaga ggcttatata aaagagctaa tagatattcc taagatggca aaagaatgta
     4021 agtctatgat gtctcagact agagaagctt taatcttatt tgatgcaagt atagcttatg
     4081 atgttggaac taaagatgat attgtagatg gattgtatga acaagtacaa attgattgtt
     4141 taaaaataat gcatgaaaac tcaaatacca tagaccaagg ggtaaaactt ctacttatag
     4201 gaagatacct tgaaagaatt ggagaccata taacaaatgt atgtgagaag ataatttttg
     4261 caattgaggg taaaatggtt gagataggat aaaaaactgc tttaaagcag tttttttatt
     4321 gtctaaatta agaaagaatt gataaaaaaa tatgattaat gggtaaattt aaaaaattgt
     4381 gatatactag gaacatatta aatatgagga tgcgtatttt atataaggtg ctttttagga
     4441 ggtggaactt tgaaacttca aaagatgggt atgaggacta taaaaacagg aattagtgtc
     4501 actttatgta tgattcttgg tgactattta gtagagaata tgttttattc tctggtagca
     4561 tgtataattt ctgttcaaga tacagtaaaa ggttctttaa agacgggaat caatagagtg
     4621 gaaggtacta ttttaggtgg tataatagga ttcatatttg caatcattaa agctggagac
     4681 ccatttttat gtggtctagg agtaatgtgc tcaatatatt tatgcaatct gtttaaaata
     4741 aagtcagtag tagttgcctg tgtaaccttt ctggctatac atctaggtgt aggaaatagt
     4801 gaccctgtgt attattcttt acatagggtg ttagatacat ctgtgggtgt tttgtttgga
     4861 gtacttgtta attattatgt tgctagacca aattatctaa atagtgttat agatgagttt
     4921 aaaaagatag aagaactttc aatgttgctt attgaagata aaattcttag aaaagaaaat
     4981 ttaaatgtag agaaatttga agaagaagta gaaaaattag aagaaatata ttcaaaactt
     5041 attgatgagt tggattatag tttgagtgaa actaatatag aaaaaattga gaatgcctta
     5101 aggatatgta gagaaataca ttttcatatg cagtctatag agttacttga aaataaactt
     5161 tatttgaaag agcatacata taaatacctt aagaaaacat ataatgtgga taagctagac
     5221 tggaatatag atgaagaaaa aagccctgta ttcaactatc atctaatgaa aataatgatt
     5281 gaaatagagt tgttgagaaa gctaaataaa tcatattaat aatagtagtt aaataataaa
     5341 taaaaaatag tatctttgta aatattaaac atatataagt ttaatttatg gaggtactat
     5401 tttttatgaa tactagaaat gacaattcta aaatactaga aatatataaa atgaagcttt
     5461 ttggcgaact tgtaaaagca gtgtggaatg atgatataga ggatattgaa aacttaccta
     5521 ataaaatttt aaatgaagat ttaaaggata atttaaataa ttattttagt ggaaaatttg
     5581 ataaagatac tatggtaaat ttaattaggg taactatggg attggatgtt acagaagata
     5641 cagataaaaa tttaaaagag tcactgcatg aagctgtaaa tttagatgaa gtcaaaagac
     5701 caataatatc tgtaatatca gatgcttgca aatattgtga tgaaaataat aaggaagatg
     5761 agtcatgtaa gataagaagt aagcatatta attgtaatga ggaagatgta tgtagtgctt
     5821 gtggtgattg tgtatcaaga tgtaagttgg gtgcaatttc agataaaata gaatttatac
     5881 caatgataaa attgttaaaa gatataaaga gccctgtata tgcaatagtt gcaccagctt
     5941 ttgtaggaca gtttgggaaa gatgtaagcc ctggtaaatt aagaactgca ttaaaaaaga
     6001 taggatttga agatatgata gaagttgctt tggcagcaga tatgcttact gcaaaagaag
     6061 catatgatta ttatgagcat atgaaaaagg acgaagaagg atgctttata actagttgtt
     6121 gctgtcctat ttgggttagt ttgatacaaa atagttttcc tcagatattg gaaaatgtat
     6181 caccatccgt ttctcctatg attgcttgtg ggagagctgt aaaaatttta aatccagatg
     6241 ctaaagttgt attcataggt ccttgtatgg caaaaaagaa agaagcaatg ttagaagata
     6301 taaaagatgc agttgatttt gtgcttacat ttaaagagtt gcaagaaata tttgatgcat
     6361 tagagttaaa tccagcaata atggatgaga gtaatagagt agaggcgtcc ctttcaggta
     6421 gagtttatgc tagaacagga ggtgtaagta aagcagttaa gttatcagtt agaagaattg
     6481 acaaaaatat aaaatttgaa gaaaaggcat ttgaaggaac aaaagagtgc atggacggtc
     6541 taaagaaagt tctaaataaa gaaatagaag ctacatttat agaaggaatg gggtgtgtag
     6601 gaggctgtgt aggaggtccg aagcgtattt taagtgtgga agaaggtact aagaatgttg
     6661 atgaatatag tgaaagtacg aatatggaaa ctccatttga taatttaaat gtattacaat
     6721 ttttaacttc tttgggaatc aaaagagtag aatctttagg agaaaaagaa gaagaaaagg
     6781 ttaaaaaaat atttagtaga gatataaaaa gtaataagaa agagaataaa tataaaaaat
     6841 aactttttaa gtcttaaact tatgctttta taaaagggag atatggggga tgaaaattaa
     6901 agataataag gctgtttttc atgtatttat aaggatattt cttatagctg ttttaatttt
     6961 aactggaaca ttattggagg aaaaaattaa taatgttcca gttgaaaata taagttcaaa
     7021 aggtgaaaat acactggaac tgctttctca aagtaatact ttaggagaaa tcatatacaa
     7081 agcatctcaa gaagaaacaa aaggtccagg ggctacttct gaaaaagtag catacataac
     7141 aatagatgat ggtccatcaa aatatacaaa tgcactactt gatattttaa aagaaaatga
     7201 tgtcaaagcg acatttttca tgcttaatca gaatatgaaa aaccacaagg aagaagtaag
     7261 gagagtcttg gaagaagaaa actcaatagg ttttcatagt gtaagtcatg atatacataa
     7321 gttatataaa agcccagaag taacagttgg ggaatttaat acatgcaata atactctaca
     7381 agaaataact ggtcagagtt caaaattaat aagattacca tacggaagta aaccatatat
     7441 gccagaaggt tcatataata aacttatggc aaatgattat ttaatttggg actggaattt
     7501 ggatacacag gattggaagt cttctacaag tcagatagta agtaatatat tatattatgg
     7561 aagaaaaaga gatgaattag tagtattaat tcatgagaaa gagcaaagtt taaatgcttt
     7621 aaataatatt ataagaatct taaaagagag aggatataca atattgccaa ttacagaaaa
     7681 tataacacct aaaaatttct ggcaagaaaa cttataataa atttaaatat agtaaaagat
     7741 aaagtaaatt atttataaaa taaaacatat aatataaaaa atacgaataa gaggtatact
     7801 gtaaggataa tataaaatta atgagaaagc attaaaatta aaataaaata atcttgcaat
     7861 tattgacatt ataagacaga ataactgtaa aaacatattt taaaatataa caactatata
     7921 aaagagaata gacataatta agaaaattca gaaaaaataa aataaaaata attgacaaag
     7981 agaataatat ttgtatataa taatatgtaa tattaaagac tgagaagaag aggagtaagt
     8041 aaatactact aaaagagagg aaaattcact ggctgtaaga ttttctaagt ataagattta
     8101 ctgaatgtag ctttggagtt tctaaactga actataagta agtttagacg gttagaaccg
     8161 ttaaattctt tgagagtcaa agttgataaa ataacattaa ccaataattt aattatatgt
     8221 taatgtattt taagctttgg aattaaggtg gtaacgcgag cttttcgtcc tttttaaaga
     8281 ggatgaagag ctctttttta tttctaaaga tatttaaaaa atttatttat aaaatacagg
     8341 aggcaggaca atggaaaata cgaatttatc aaaaacttat aacccaaaag attttgaggc
     8401 taggctatat aaaaaatgga tggatgaagg gtattttaaa tctaaaccaa atccagataa
     8461 aaaaccattt acaataatga tgccaccacc aaatataaca gggcaattgc atatgggaca
     8521 cgctcttgac catacacttc aagatatatt aatcagatgg aaacgtatgg atggatatga
     8581 agctttttgg cttccaggaa ctgaccatgc ttctatagca acagaagtta aagttgttga
     8641 aagaataaaa aaacaagaag gcaagacaaa gtatgaaata ggtagagaag aatttttaaa
     8701 gagagcctgg gaatggaaag atgaatttgg aggaaaaata tctaatcagc ttaaacaact
     8761 aggtgattct tgtgattggg ataaagaaag atttactatg gatgaagggt gtaatgaagc
     8821 agttatagaa ttttttgtaa gcctatatga aaaaggtcac atatacagag gaaatagaat
     8881 aataaactgg tgcccagatt gcaaaactac tttatcagat gcagaagttg aacatgaaga
     8941 acacgacgga aacttttatc atataaagta cccactaaaa gatagtgaag actttttaga
     9001 aatagctaca actagacctg agacaatgat aggagatact ggtatagcag taaatccaga
     9061 agatgataga tataaacatt taataggaaa aacagcaata cttccattag taggaagaga
     9121 acttccaata gttgcagata gttatgttga tttagagttt ggaactggtg cagttaagat
     9181 gactccagca catgacccta atgattttga agtaggatta agacataatc ttgagcagtt
     9241 aaatactatg aatgaagatg gaactatgaa tgaagtttgt ggaaaatatg aaggtatgga
     9301 tagatttgag tgtagaaaag ctatcgtagc agacttaaaa gaacaaggtt acctaattaa
     9361 gataaaagag cataatcata atgtaggaac ttgttataga tgccatacag tagttgagcc
     9421 tagattatca gagcaatggt ttgttaagat ggaagaactt gcaaaaccag ctatagatat
     9481 tcttaaaaaa ggagaattag aatttgttcc agataaattt gataaaactt atttacaatg
     9541 gctagaaaat ataagagatt ggtgtatatc aagacaatta tggtggggtc atcaaatacc
     9601 agcttattat tgtcaagagt gtggagaaat agtagttgct agaaaaatgc cagataagtg
     9661 tcctaaatgt ggaagcactc attttaaaca agatgaagat gccttagata cttggttcag
     9721 ttctgcatta tggcctttct ctactctagg atggccaaat aagactgaag ctttagatta
     9781 ttattatcca acaagtgtac ttgttacagg atacgatata atattcttct gggtagttag
     9841 aatggcattt gcaggtatgt tctgtatgaa tgaaaagcca tttgaccatg ttttggttca
     9901 tggattagta agagattctc aaggtagaaa aatgagtaaa tctttaggaa atggtatcga
     9961 cccattggag ataatcgagc aatatggagc tgatgcccta agatttacat taacaactgg
    10021 aaattcccca ggaaatgata tgagattcta tatggaaaga gtagaatttg ctagaaactt
    10081 tgcaaataag ttgtggaatg catcaagatt tgtatttatg aatattgatg aagatataat
    10141 taaaaacatg actagagaaa gtgttaaaga ggacttaact ctagctgata aatggataat
    10201 atcaagagca aataatattg taaaagaagc aactaataac atggataagt ttgatttagg
    10261 aatagcactt caaaagatat acgattttac ttggtcagaa tactgtgact ggtatattga
    10321 aatggttaaa cctagattat atggggaaga tgcaaatgct aaatctgcag cattatatac
    10381 attaacttat gttcttgaaa agatattaaa attattacat ccatatatgc catttataac
    10441 agaggaaata tatactcatc ttcctactgt tgaaggatgt ataatagtat ctgaatggcc
    10501 aaaatataat gaagaagata acatggctga agaagaagat atgatgaact tattgatgga
    10561 aggtataaga agtataagaa atgtgagagc agaaatgaat gtaccacctt ctaagaaagc
    10621 taaattgata ataatcccaa gtgaagaaaa aatagaagct attgagcttg gtaaagacta
    10681 ttttataact ttagcatctg cttcaaatgt agagatagca aaagataaat ctaatgttcc
    10741 agaagatgca gttggagtag ttattgatgg tgttgaaata tttatacctc ttaatgaatt
    10801 agttgatttt gaaaaagaaa ttgagagatt atctaaagaa aagaaaaagt tagaaggcga
    10861 aataaagaga gttaatggaa agcttgcaaa tcaaggtttc ttagcaaaag ctcctgaaag
    10921 tttaattgaa gaagaaaaag caaaaaaaga aaaatttgaa gagatgatta aatctgttga
    10981 ggaaagatta acaaatttag aatctaaaat aaaataaata taagaattag taaaaacata
    11041 caaaaagtat atattctata ctttttgtat gtttttcttt atatatatac aaatatgatg
    11101 t
//
LOCUS       DAMJVI010000111        10873 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.116, whole genome shotgun sequence.
ACCESSION   DAMJVI010000111 DAMJVI010000000
VERSION     DAMJVI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.116"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(5..1063)
                     /locus_tag="PC545_003068"
     CDS             complement(5..1063)
                     /locus_tag="PC545_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDF2457812.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSSQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVRVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSKIQKLLPLIDYKNIILDE
                     KNKSIKLTKENMKIDLGAIAKGYAADKIVAYLKSEDVKKGLVNLGGNIFTLENGKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMNLINKIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKSFSIEKLNKQ"
     gene            1130..1501
                     /locus_tag="PC545_003069"
     CDS             1130..1501
                     /locus_tag="PC545_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDF2457813.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIESRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            1538..2212
                     /locus_tag="PC545_003070"
     CDS             1538..2212
                     /locus_tag="PC545_003070"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDF2457814.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            2461..3300
                     /locus_tag="PC545_003071"
     CDS             2461..3300
                     /locus_tag="PC545_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HDF2457815.1"
                     /translation="MNFKKLSNAKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDSNDIVTLDENTKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     ILK"
     gene            3544..3640
                     /locus_tag="PC545_003072"
     tRNA            3544..3640
                     /locus_tag="PC545_003072"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3578..3580,aa:Sec,seq:tca)
     gene            3795..4502
                     /locus_tag="PC545_003073"
     CDS             3795..4502
                     /locus_tag="PC545_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HDF2457816.1"
                     /translation="MENNFTTRTSFLVGDDGIKKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NKDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIVNELLGQ"
     gene            complement(4644..5480)
                     /locus_tag="PC545_003074"
     CDS             complement(4644..5480)
                     /locus_tag="PC545_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2457817.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEDSENIIISYLRQKRIKTIDIIIATHPDSDHIGSLDNIIKKFNVNSIY
                     MPEQSTDSKAYQNLINSCTDKNLSIQHLYKNDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNDNSFLFMGDAEEENEKEILHSFKLNNINFIKIGHHGSNSSSSLEFIKKIS
                     PDIAAISCGYKNQYGHPHREVINNLKQNHVSIYRTDRIGDIVFYSDGEIIFTKYNYEI
                     DT"
     gene            5668..6573
                     /locus_tag="PC545_003075"
     CDS             5668..6573
                     /locus_tag="PC545_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HDF2457818.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPGRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYEAKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            6582..7766
                     /locus_tag="PC545_003076"
     CDS             6582..7766
                     /locus_tag="PC545_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457819.1"
                     /translation="MEIRYAKEEEIESIKDIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNSKVYNTSYVVGVSTLPEGRGAGYMNKVMKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     QIDRTFLNEVNGNVLKDEHYYENLFKEIQSEDGFLYIHEGNEKDGYIVYFLQEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPTIDKIRFILDNPKDSDIKIKPFMMG
                     RVINVKKFIEDIDIEKDINSSFNLLIEDKFIDENNGLFKISIQNKKVSVEQLDKKGAE
                     KPQEDFDIKLDINTLTQLSFSYIDVNEAIFLNDIKDVSEETLETLNCIFSKKNNYINE
                     YI"
     gene            7881..9182
                     /locus_tag="PC545_003077"
     CDS             7881..9182
                     /locus_tag="PC545_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HDF2457820.1"
                     /translation="MGVIIIQIRRPLLIILMFVVAISFIITNNTKNDEELNDKIITIQ
                     GVVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKLYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLTEEQKEIFTRTGTSHVISISGLHTGILCVIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIMYLSIASLIVFKVSVVIAKIISFFNSTFIESIFFLLEKISNLSFAYINIENPKF
                     YIVVIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKL"
     gene            9136..10179
                     /gene="holA"
                     /locus_tag="PC545_003078"
     CDS             9136..10179
                     /gene="holA"
                     /locus_tag="PC545_003078"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDF2457821.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGNNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(10366..10632)
                     /gene="rpsT"
                     /locus_tag="PC545_003079"
     CDS             complement(10366..10632)
                     /gene="rpsT"
                     /locus_tag="PC545_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDF2457822.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
BASE COUNT         4257 a         1122 c         1679 g         3815 t
ORIGIN      
        1 aaacttattg tttatttaat ttttcaatag aaaaagattt atctgtaagt ttaaaattat
       61 cttttatacc cttagttaag tatactttct tatccttagt tatgaatata gcatctacat
      121 tatcaatttt attaattaaa ttcatacctt tttcaagacc aagaccaaat gtagaagtgg
      181 ataaggcatc acaattaata gatttatcag agattattgt aacactactt aaattgtttt
      241 caaaaggata ccctgtaaat ggatttaaca tatgatgata tatttttccg tctttttcga
      301 taaatctttc ataaattcca gaagtaacaa cagacttatt tgtagtttct atgtttccta
      361 tggattcacc atttttacta gttgggtctt gaatacctat tttaaaaggt ttatcatttt
      421 taccattttc taaagtaaat atatttcctc ctaagtttac taaacctttt tttacatcct
      481 ctgattttaa ataggctaca attttatcag cagcatatcc tttagctatg gcacctaaat
      541 caattttcat attttctttg gttaatttta tagatttatt tttttcatcc aatatgatgt
      601 tcttataatc aataagaggc agaagttttt gtatcttaga atcacttgga actttagctt
      661 tatctgttcc aatagcccat aaatttgata atggaccaat tgttatgtca aaatatccat
      721 ctgataattt ggaatattgt atagcttctt ttacgacgaa aaatgtatca tcagaaactc
      781 tgacaaattt tttaccagca ttatcattta ttttagaaac atcacttcct ggtatatgag
      841 tgctcatttt gttgtcaata tatcttaaaa ttgaatcaca ttcatttaag attttatcac
      901 ttttggattc ttttacatta aatacagtaa cttcgtttac tgtaccaagg tagtagctag
      961 ttttactata tttagtattc tggcttgaac tcctaaaaaa tgaaaataaa attaatgcta
     1021 aaatcaatat cacaaaaata aatgtgattt ttttcttatt catggtatat tcctcctatg
     1081 aaattgtgtt attattttat tataacataa tttcatagga ggacaaaaaa tgaagaaaaa
     1141 agacattctt cttattttag gcattttggt tgttattaca gcatgttatg ggataataaa
     1201 tgttataaat tctaaaaatg ctggaaatat tgagatttat gtagataata aattgtataa
     1261 aactgtttca ataaatgcca aagaagaatt taaaatagag agtagagggg ggtataacat
     1321 agtaaagatt catgataaag gggttgagat agtagatgct tcatgccctg ataaggtttg
     1381 cgtacatact ggatttataa acaagccaag tcaaagcata gtttgtatac ctaatagagt
     1441 gagcataaaa ataaatacaa atgaaaagaa tgataatcaa gaagatataa tttctaaata
     1501 aaataataaa aataattgaa aggaattggt gataattatg gttaacttag gaaacgattg
     1561 ggatgaatta ttgaaagaag aatttgaaaa agattactac ttaaacttaa gaaaatttct
     1621 tatagaagaa tataaaacac gccaaatttt cccaaatatg cacaatatat atgaagcttt
     1681 aaaacacact tcatacaaag acacaaaagt cttaatatta ggacaagacc cttatcatgg
     1741 agataatcaa gctcatggtt tagctttttc agttcaacca caagtgaaaa ctccaccatc
     1801 tttattaaat atgtataaag aattaaaaga tgatttaggt tgttttatac ctaataatgg
     1861 atatttaatg ccttgggcag accaaggagt gttactttta aatactgctc ttactgttag
     1921 agcgcatgaa gctaattctc ataagaacaa aggatgggaa attttcactg atagagtaat
     1981 atctatttta agtgagcgtg aagacccagt tatttttgta ctatggggaa gtaatgcacg
     2041 taaaaaagta gagttgattg atacaagtaa acattatatt ttggaagcac ctcatccaag
     2101 cccattgtca gcaagtaaag gcttttttgg atgtaaacac ttctctaaga taaatgaaat
     2161 tttgaaaaag ctaggaaaag aacctattaa ttggcagata gaaaatatat aaaagtaaaa
     2221 ggtaacaaaa tgataacaaa acaacaataa acaatgacat tgttgtaact aatagaaata
     2281 tgcttaaata gttgaaaata ctatgtatgc atatttctat ttttttgtca agttgacaac
     2341 tcttgaaaaa atagttacaa tttagttaca caaaaaacac aaataaaaaa agaaacttac
     2401 aaagcagaat tttatgaagc aaaatatata gtgtaaaaaa tcttagggag gaaattttat
     2461 atgaatttta aaaaattgag taatgctaaa gtctcggtaa tggcagttgt tttatcagtt
     2521 ggaatattat cgggatactc tgcgcttaat aaacaagtaa cattagtagt aaggggagaa
     2581 gaaagaacta tatcaacatt tagttctaat gtaggagacc ttttagaatc tcaaaacatt
     2641 agctatgatt caaatgatat agtaacttta gatgaaaata caaagttatc taatggagat
     2701 aaaatagaag taattgatgt aaaggaaaaa acagttgtag aaactaaaga cattccattt
     2761 gaagttacag ttgttgaaga tggctcttta ttaaagggtg attctaaggt taaagaagaa
     2821 ggaaagtctg gtaaaagtga attaatatat aaagaaactt atcacaatgg aaagaaagta
     2881 gaaaaggaat ttgtgaaaaa ggttgttaaa gctgagccag taaataaggt tgtaagcaaa
     2941 gggacaaagg tagaaatgca agtagcctca tcaaggggag aaagttcaag aagaatggct
     3001 tcatcatcat ctagtagcag tagtagtaac aatggtaaaa atatgagagt ggtagcaact
     3061 gcatatactg gtcacagtat aactgcaaca ggaacaaaac ctaagtgggg aacaatagct
     3121 gtagacccta aggttatacc atatggaact aaagtttata tacctcaatt tgacatggtg
     3181 tttactgcag aagactgtgg tggagcaata aaaggaaata aaattgatat ctacatgaat
     3241 gattcaaaaa cagtttataa ctggggaaga aaaactatag atatacgtat attaaaatag
     3301 ttaaataatt tatattttta ctggaaatta tattttgatt atatagagaa gaaactcttt
     3361 atagattcta aaaatataat ttccagtttt ttattttgga tatgtaataa aacaggtaaa
     3421 ttttaaccat gtatagataa aagctattta aattataatt tactatcata taaatctata
     3481 aaaattgttg caaagagtca aaatatatga tattatagat ttcgttgaga aggatttcat
     3541 tatgggagtg gatgggtgcc ggtgtgccct ctagtcttca aaactagtat gaggggttaa
     3601 gagcctctta ggtgggttcg attcccacgc attcccgcca atatgtaaaa ggtatctttt
     3661 aattaaaaga taccttttta aatttatttt aagataattt atttttaata atatataatg
     3721 ttttgtatta tataatgtaa attttctaat acaaaacatt atatatttag ataaaaatct
     3781 aggagtgtgg aaagatggaa aataatttta caacgagaac tagttttcta gttggagatg
     3841 atgggataaa aaaacttaat aattcaaata taatagtatt tggagttgga ggagttggta
     3901 gttttactgt tgaagcttta gcaagagctg gagtaggcaa tattactatt gtagattttg
     3961 atgatgtaga tataaccaat ataaatagac aaataccagc cctacattca actgtaggaa
     4021 gatacaaagt tgatgtcatg gaagaaagaa tattagatat aaatcctaat atcaatataa
     4081 aaaaaataag aagtctttat aataaagata ctagtgacga aatactaact gaaagatatg
     4141 attatgttgt ggatgcgata gacatggtaa gttccaaaat acatctaatt gaaacttgtg
     4201 aaaaaaaagg attgaaaata ataagttcta tgggtatggg aaataaatta gacccaacta
     4261 aaatagtcgt tacagatata cacaaaacaa gtacttgtcc tctagctaaa gttatgagga
     4321 aagaacttag ggatagaggt atcaaaaagt taaaagtagt atattctact gaacaaccaa
     4381 tagaacttaa gaagaaagta atgaatggta gaaaagtaac acctggtagt gtatcatttg
     4441 tcccatctgt aggaggttta ataatcgcat ctgtaatagt aaatgaacta ttaggacaat
     4501 aagttttaaa tattttaatt tataaatttt atatacaaat ttgaaggcta gaagcatgaa
     4561 tacaaagcaa ttttatattc atacttttag cctaattatt ttatattacc ttattttatg
     4621 tagttgtagt tttatttttt agattatgta tctatttcat aattatattt tgtaaatata
     4681 atttctccat cactataaaa aacaatatct cctattctat ctgttctata aattgataca
     4741 tggttttgtt ttagattatt aataacctcc ctatgtggat gaccatattg atttttatat
     4801 ccacatgata ttgcagctat atctggacta atttttttta taaattctaa agatgaagaa
     4861 gaattacttc catgatgacc tatttttata aaattaatat tatttaattt aaaagaatgt
     4921 aaaatttctt tttcattttc ttcttcagca tcacccataa ataaaaaaga attatcatta
     4981 aaagttaatt tgaaaactat agaatttaaa ttagattctt cttgtatata tgatggactt
     5041 aatacatata tatttatatt gttatctatg tttaaaacat catttttata gagatgctga
     5101 atagataagt ttttatccgt acaagaattt attagatttt gataagcctt actatcagtt
     5161 gattgttcag gcatatatat ggagtttaca ttaaattttt ttataatatt atcaagactt
     5221 cctatgtggt ctgaatctgg atgagttgct attataatat caatagtttt tattcttttt
     5281 tgtctaagat aactaataat tatattttcg ctatcttcat cacctccatc aattaatata
     5341 ttcttatttg tgggtgtttg aactaaaata ctatcacctt gtccaacatc aatcatatgt
     5401 atagatagta gagatttttt gtcacagccg attaaaagtg atattataat aagtattgat
     5461 aaaaaaattt tatttctcaa taaaaaatca ctccattcat tacatgttta tataaattta
     5521 tgatgggtaa tataagatat atatgactta ttatatttta tattttaaac aatataaatg
     5581 aaattaatac cataataaaa ttggagtttt ttatatatac aaaataaaat ttttaaaatt
     5641 aagcaataaa aaaggtagga ggattttatg tttttcaaag atattgaact aaattcaaaa
     5701 aaagagttag acccatattt tgatttagta gattatgaag cttgtgagta ctgttttagt
     5761 actttatata tgtggcaaca tgtttataag acaggatatt atataggtga agattttgcc
     5821 gttttagttg gggaatacga aggagatagc ttctcaatat taccacttgc taaaaaagat
     5881 aaacttccag aagttgtaga ttttgtcctt gaatattttt ctaaaaataa taaaaaaata
     5941 tacttaagag gtattactac tgaagtagta gagtttttaa aagaaaaata tccaggtaga
     6001 tttgagtata tagaagaaag agatttattt gactatattt atgatgcaga aagtttaaga
     6061 actctagctg gtaaaaagaa tcaaaagaag agaaaccata taaactattt cttaaaagag
     6121 tatgcaggta gatatgaagc taaattatta gataaagaaa actttgatga gtgtttagta
     6181 ctaatgaagg aatgggaaag taataaggaa gaaaataatg aatttgatga aagtatggat
     6241 gatgaattaa taggtatcaa aaagatattt aaccattatg acatattaaa agataaggta
     6301 aaagtgtttg gtgtttatgt agatggaaaa ttagaagctt ttagtatagg agagctttta
     6361 aatccaaata tggcattgat tcatatagaa aaagctaatc ccgatataag aggtctgtat
     6421 ccatttataa atcaacaatt tttggtaagt gaatttaagg acgttgagtt tgtaaataga
     6481 gaagaagatt taggtattga aggattaaga aaagctaaac tttcttatca tccttgcaga
     6541 tttgttgaaa agtacagtgt tagggaggct taatagacta tatggagatt aggtatgcta
     6601 aagaagaaga gatagaaagt ataaaagata tttggagtta ttgttttaac gatacggaga
     6661 gttttatgaa gtactatttt aatgataaat ataaatctga aaatactgta gtagctttag
     6721 atgaaggaaa aattatttct tctcttcagc taaatcagta taagttatta ctaaatagca
     6781 aggtatataa tacatcatat gtggtaggag tttctacact tccagaggga agaggagctg
     6841 gatatatgaa caaagtaatg aaatttactt taaacgaatt gtataaaaaa gggcaattag
     6901 tttctatatt gatgcctatt gactatagac tttatagaag atttggatat gagcattgtt
     6961 acgaccagat tgaatatact attaacacag atgatttaaa aaattttaag tcaagtggaa
     7021 aaatgataaa gtctaattta tcacaaattg atgatttgat acaaatagat agaacttttt
     7081 taaatgaggt taatggtaat gtgttaaaag atgaacatta ttatgaaaac ttatttaaag
     7141 agattcaaag tgaagatgga tttttataca ttcatgaagg aaatgaaaaa gatggatata
     7201 tagtttattt tcttcaggaa gataaaatgt ttgtcagaga gttattttat aaaaatatag
     7261 atgcccttaa atcaatgctt aaatttatat ataatcacaa tactcaatgt aagatagtta
     7321 ctatatctac tcctacaata gataagataa ggtttatatt agacaaccct aaagatagtg
     7381 atataaaaat aaaacctttt atgatgggaa gagttataaa tgtaaagaag tttattgaag
     7441 atatagatat tgagaaggat attaatagct catttaattt attaatagaa gacaagttta
     7501 tagatgaaaa taatggtcta tttaaaattt ctatacagaa taagaaggta tctgtagagc
     7561 agttagataa aaaaggtgca gaaaagcctc aagaagattt tgatattaaa ttagatataa
     7621 ataccttaac acagttatca ttttcttata tagatgtaaa tgaagctatt ttcttaaatg
     7681 atataaaaga tgtttcagaa gaaacactag aaacacttaa ttgtattttt tctaaaaaaa
     7741 ataactatat aaatgaatat atataggtag agtacgttaa aagattctaa aaatcttttg
     7801 acgtattttt ttgtttagat aacaatttat cttgtttaaa aattttctat ttgaaaataa
     7861 tctaaataat atacttttat atgggagtga ttataattca aataagaaga ccattattaa
     7921 ttatattaat gtttgtggta gctatatcat ttatcattac aaataacaca aaaaacgacg
     7981 aagaacttaa tgataagata ataactatac aaggagttgt caaaggaaaa attgaaaaga
     8041 aaagatacaa tcagtacaag gtaggggttt ttctaataaa tgattataca aaaaataaaa
     8101 atttgaagat tggtcagaaa gttaatatta caggtaaatt caaaagttta gataaaatga
     8161 agtatgatga ttttgattat ggaaggtata taaaaagtac tggatataaa ggtatagtgt
     8221 atataaataa ctataaaatt attgataaaa ataaacttta ctctttaata ggggaaataa
     8281 agttttatat tagtaaaact tataggtact tatataagaa aaactcagat tttataaatt
     8341 ctatactatt agcagaagtt gaaaatttga ctgaggaaca aaaagaaatt tttaccagaa
     8401 ctggaactag tcatgtaata tctatatcag gtcttcatac aggaatatta tgtgtaatta
     8461 tatcctttct attaagaggt ataaataaat tgtataaatt attaatactg tcgattttta
     8521 taactttata ttgtataatg gtaggtgcgt ctccatccat aattaggtct atagcatttg
     8581 ttatgatttt ttatttatca atatttattg atagaaagaa agatggaata tcggcattgt
     8641 ctctaattgg gataatttta attatgaata acccctatgt tatatataat gtaagttttc
     8701 aattgtcttt tttagctaca ctttctatat taatattcta taataaaata aatagtatta
     8761 taaaattaag tatggtatca ttgacaatat catcaaatat attaacttta cctataattt
     8821 attatacatt taaaggtata ccattgcttt caattatagg aaatttaatt atagtgccat
     8881 ttgtgggagt aataatgtat ttaagtattg caagtttaat cgtatttaaa gtaagtgtag
     8941 taatagctaa aattatatct ttttttaata gtacatttat agaaagcata ttttttttac
     9001 tagaaaaaat cagcaattta agttttgcat acattaatat agaaaatcct aaattttata
     9061 ttgttgttat ttattacata ggagtgtttt tttacatatt ttacatagaa ggaaaagaga
     9121 taaaggagca agagaatgaa ttacaaggat attataagga atgtaaaaga gaaaaacttt
     9181 gaaaaaatgt atctttttta tggaagagag tattatttaa ttgagaatgc aataaaagca
     9241 tttaaagata gtctaaacga gggaatgctt gattttaatt tagatataat agatgggaag
     9301 gaaatagtct taaatcagct tataagctct atagaaacac ttccatttat ggatgatagg
     9361 aagatagtaa taataaaaga ctttgaacta ttaaaaggaa agaaaaagaa ttttacagat
     9421 agtgatgaga aatacttaat agagcattta gacaatattc cagacaccac aactatagtt
     9481 tttgttgtat atggagatgt agacaaaaga aaatcattag ttaagaaaat aggaaacaat
     9541 ggaattgttt ttgattgtga taagctttct gatatggatt tgtttaaatg gataaaaaaa
     9601 tcttttgctt taaatgatgt tataatagat aattctcaaa tcatgtattt tattgaacaa
     9661 gaaggataca gagataagag tagtgaaaag acattatctg atttagaaaa tgaaataaat
     9721 aaaataagtt catttgttgg taaagggaat aatgtaacca atgatgtaat tgataaatta
     9781 tctcaaaaaa aagttgagaa tgatatattc aaattaatcg attatattgg agaacaaaat
     9841 gcttctaatg caatgaaaat attaaatgat atgatacaag aaggcgaatc tgtattaggt
     9901 atattttcta tgatagcaag acaatttaaa ataattatgc aagtaagaca gttacaactt
     9961 gatggatatt caacaaaact tatagctgat aaattaaaaa tgcatcaatt tgttgtagga
    10021 aaagcattaa agcaaactaa aaatttttct gatgatataa ttgtggaaat attaaactat
    10081 atattagaaa gtgattacaa aataaagaca ggtcttataa gagatacttt agcagttgaa
    10141 atgttggtta gtagatactg taaaagagaa gcaatttaaa aaatctatat attaataaaa
    10201 aataaatagc aagatatgtt atagttcaga gtagatgtaa gctaaattaa gtttgatata
    10261 caatcaacta taatatgtct aactatttat tttttattgt ataaatttac aaaataaaaa
    10321 acccctacat aggggttatt atatataaga aatataaact ggtaattaag cgttcatacc
    10381 gtttaatttt tgagctaatt tagctacttt tctagctgca gcattcttat gtatagttcc
    10441 tttagaagct acttggtata ttcttttttg agcgtattgg aacttagcaa cagcatcctc
    10501 tctgtttcca gctgcaacag catcttcgaa tcttcttatt gcagttttta tttgagattt
    10561 tctaactcta tttaaagcag ttttcttttc gataacactt attcttttct tagctgattt
    10621 tatatttgcc accgtgttca cctccctgta tttttttatc atattcatcg tcaacatatt
    10681 agattttaac agaaataaat ctaaaaatca aggattatta tttatatatt ttaataatgt
    10741 tttttatttt gattttttag actgtaaatt gtgtataaat aatttgcaat atcttgtttt
    10801 taaattggta tattttgtgt tttaaatatt ttgacatata taattaatat ttcattttgt
    10861 aacaaatatt tga
//
LOCUS       DAMJVI010000112        10821 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.117, whole genome shotgun sequence.
ACCESSION   DAMJVI010000112 DAMJVI010000000
VERSION     DAMJVI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.117"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(342..1349)
                     /locus_tag="PC545_003080"
     CDS             complement(342..1349)
                     /locus_tag="PC545_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457823.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     KQEMTVMKEDMSEVKQEINVLKGDMSEVKQEMTVMKEDMSEVKQEITVMKEDMSEVKQ
                     EINVLKVDMSEVKQEMTVMKEDMSEVRQEINIMKEDMSEVRQEINTMKEDMSEVRQEM
                     TVMKEDMSEVRQEMTVMKEDTSEVKQEINVMKKDMSEVRQEITVMKEDVSEVKSEVNF
                     MQNKINNINEDMNGIKDEVSIANEKLYGIEIKIDSLECEDKSMKEIQIEQNNILGSLL
                     HNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELATSKNWSDIVKLKSV
                     K"
     gene            complement(1579..2664)
                     /locus_tag="PC545_003081"
     CDS             complement(1579..2664)
                     /locus_tag="PC545_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDF2457824.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            complement(2700..4013)
                     /locus_tag="PC545_003082"
     CDS             complement(2700..4013)
                     /locus_tag="PC545_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2457825.1"
                     /translation="MIAIKSKYLISSADEIYTNSAVVVENDIIKDILPNEEVEIKYKN
                     IEEIIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAILSLESCE
                     RISFENGLRCLNENSNLIKWSRENSKLINGIMCTHTSFTCSDRFIKKAKEDTKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAYVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWKF"
     gene            complement(4014..6305)
                     /locus_tag="PC545_003083"
     CDS             complement(4014..6305)
                     /locus_tag="PC545_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2457826.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPNYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSKALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGSDKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGRTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVRDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKQKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            complement(6295..7089)
                     /locus_tag="PC545_003084"
     CDS             complement(6295..7089)
                     /locus_tag="PC545_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2457827.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKDLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMNLADFLENELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVVRMYNSIGGECDGK"
     gene            complement(7128..7574)
                     /locus_tag="PC545_003085"
     CDS             complement(7128..7574)
                     /locus_tag="PC545_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HDF2457828.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVEGQHITTLEGVLDEAKKFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            complement(7646..9052)
                     /locus_tag="PC545_003086"
     CDS             complement(7646..9052)
                     /locus_tag="PC545_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457829.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHNHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTEKD
                     MELTKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTANEEKALKDAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            complement(9070..10440)
                     /gene="hydA"
                     /locus_tag="PC545_003087"
     CDS             complement(9070..10440)
                     /gene="hydA"
                     /locus_tag="PC545_003087"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2457830.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDEEIVEIGNNLEINGDKVID
                     ATGKIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKASGNCYCDYGFHMTITCLNKDTFNHMEKLISDGIVSFKMYMAYDGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLDVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCRESLETVKMAREKNIDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINYLWEGLSSGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKVKCKVEDVFLRGNHVVQSCNVKEHPTGQYI
                     KRKITK"
     gene            complement(10573..>10821)
                     /locus_tag="PC545_003088"
     CDS             complement(10573..>10821)
                     /locus_tag="PC545_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2457831.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIKTIG
                     FGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSVYGK"
BASE COUNT         3154 a         2173 c         1126 g         4368 t
ORIGIN      
        1 taatagtaaa aattttattc atattactat ttataatttt tatagagcaa ttttagtgcc
       61 aatatttgca tacatttgta tagtataaaa tttttatgtt ctcattgata aaattttact
      121 tatttcaagc gttctattag attttatcat ctaaatttta ggttttattt ttataaatta
      181 attgtattta cataaaaaaa aagacctaaa aacaaatttt taacggtctt ttctcaattt
      241 tttattttca tttctaaaat ttataataaa tagtaagaac actataaatt cttttatata
      301 aaattcttag tttgtggatg ataaattagt gaattaaaat actactttac tgattttagt
      361 ttgacaatat cactccaatt tttactagtg gctaactcta ctaaatttaa gtctcttctt
      421 atttcagcta tatcctcttt tatagaatta gtgtcacctt ttatatatgc tatgttatgt
      481 tctatattat catgagtagc tttatttatt tcagaattat gtaataaact tcctaaaata
      541 ttattctgtt ctatctgtat ctctttcatt gatttatctt cacattctag actatcaatc
      601 ttaatttcaa ttccatataa tttttcattt gctatggata cctcgtcctt aattccattc
      661 atatcttcat taatgttatt tattttattt tgcataaagt ttacttctga tttaacttcg
      721 gaaacatctt ctttcattac agttatttct tgtctcactt ctgacatgtc ttttttcatt
      781 acatttatct cttgttttac ctctgatgta tcttctttca ttacagtcat ttcttgtctt
      841 acttcggaca tatcttcttt cattacagtc atttcttgtc ttacttccga catatcctct
      901 ttcattgtat ttatctcttg tcttacctca gacatatcct ctttcattat atttatctct
      961 tgtcttacct cggacatatc ttctttcatt acagtcattt cctgttttac ttcggacata
     1021 tctactttta atacatttat ttcctgtttt acttcggaca tatcttcttt cattacagtt
     1081 atttcttgtt ttacttccga catatcttct ttcattacag tcatttcttg ttttacttcc
     1141 gacatatctc cttttaatac atttatttcc tgttttactt ccgacatatc ttctttcatt
     1201 acagtcattt cttgttttac ttccgacata tcttctttta acacatttat ttcttgtttt
     1261 acttccgaca tatcattttt tatgctaact acatcttcat ttatagtata taataactct
     1321 aaaatcttct cttgcacttg catcaccatc cctattaatt attataacat tttacacaaa
     1381 tgctatacaa tgttaacata tatatcagaa ttattaaaat tttaattaaa caaaattgat
     1441 tcactttatt acttcaaaac taattaatat ctaaaataac tacaaataaa caaaaataag
     1501 gtcaacaaca aaaaaggctt atctctttga gatagccttt cttattttta tttaaaactt
     1561 tgttttttat aaaataatct atacattcag ctctgaacat gaaatttttc ttccaagtgc
     1621 ttttggttta caaactgata tacaaagtcc acaatatcta cactcttctt cattcaattc
     1681 catcttatca ttcaattttc tcgcttgata tgcacaaact ctctcgcaca tcttacattt
     1741 agtacatttt tcttcatcat aagtaaactc aaagctaagt ctttcattaa catctcccat
     1801 atgattatgt actataccac aagcattttc tatagtatta tatcctaatc tattaagatt
     1861 agattttaaa tcatcatgta ttttagatat tacttgtgcg ccttttaaaa ttactactga
     1921 acatatacca acaaagtcag cacctgccat aatcatttct aaggcatcat ctccacttac
     1981 acatccacct aagccaatta ttggtatatt aacttgtttt tttatttcat atattctctg
     2041 taaagttatt ggcttgatag tttctccact taaatatcct attccatttc cacctaataa
     2101 aggttgccct gtttctatgt ctattctaaa tgctggtcct acagaatcac aagcagttat
     2161 tgcatctgct cctgcttctt ctaatttttt agcaaaatcg cctatctcat ctatcatagg
     2221 aggaagttta actattacag gtatatttac tctttccttt gcatctttaa gcattggtat
     2281 caaatctcta tagtcataag atactaattc aataaagtca gctcctgcat ttgcaacttt
     2341 ctctactaat tcgctacttt cttcaattgt atgacctact gaagctatac agacagtccc
     2401 atctcttttc atttgtggta tttcaaaatc tatccattgc tctggctcaa aatctgtcca
     2461 tttttcatta tttaaaagag cattagctgt atttctaacc atatgtggtg ctggatttat
     2521 agctctttct tttcttatag tcttagtcac aactgcccct gctcctgcat cacttaatat
     2581 cttgcatcca gctgcactat atgttaatgg tcctgaacct attataagtg gtgattttaa
     2641 ttctttacct aaaaaattat acattatctg cttccccttt tcatatatat ttacattcgt
     2701 taaaatttcc aaaattcaca agcaacttct ttcatttctt taacaatagc ttttttatct
     2761 aaatcaacta actgtttttc ttttaaaata tgtctaccat ctatgtatac atttgatata
     2821 aattcttttt taccctgaac tactatctgg tcaaatatat tatcaagagt cacaggagtt
     2881 ttaaattcat cttccataac tattatatca gccttattac caactgctat tttaccagaa
     2941 ttttgaagtc ctaatacata agcaccatgc tctgttgcca ttctaaatac taaatttgct
     3001 ggcattactg atgcatcttc ttcaactgat ttatgcatta aaaatgcacc tctcataact
     3061 tcaaaaaaat catttatata tccgtcagta cccagtgcca cttttatacc ttttttaatc
     3121 atttttggta ctggagaaaa acctccacct acttcacagt tgctaagagg catatgaacc
     3181 acttttacac ctttttcttt taatatatct atttcttcat catttacttt tacacactga
     3241 gatgctaata cagtttcatc caatatatct aaatcattat agtagtcaac tgcttttttg
     3301 ccaaaatgtt tttcagcata gtttggctca tatatactct cacataaatg aaattgcatt
     3361 ggtgcattta atttttttgt atcttcttta gctttcttaa taaatctatc agaacatgtg
     3421 aagcttgtat gtgtacacat tattccattt attaatttac tattttctct actccacttt
     3481 attaaattag aattttcatt taaacatcta agtccatttt caaagcttat tctttcacaa
     3541 ctttctaagg ataaaatcgc tttcatgcct atttcttcta gtatttttcc ttgctcaatt
     3601 aaagttcctt cttctgtatt tggagcttct agggtatcac aaaaagctac cacaccagat
     3661 tctataagtt ctattgctga tgctttagtt gttgccttca cttctttcaa tccaattcta
     3721 ttttctataa atggccacca atagtcattt aagaaacctt caaagtcggt aaaatgtaca
     3781 tttgccggta ttcccctaga aagaactcca tattgatgca tatgaccatt gacgaatcct
     3841 ggcattatta tagcatctga tttatctatt atttcttcaa tatttttata ttttatttca
     3901 acttcttcat ttggcaatat atcttttata atgtcatttt ctactacaac agcagaattt
     3961 gtatatattt catcagcaga agatattaag tatttacttt taatagcaat catttattta
     4021 cctccctgat ttaatttttt catggccata taaactttct cagaagttat aggaagttct
     4081 cttatacgaa ctcctgtggc attgtatatt gcatttgcta ttgctggtgc aggtgtatga
     4141 ataactatct ctcctaaaga tttagctcca tatggacctg ttggctcata attatctaca
     4201 aaatcaacaa ttatgtttcc tatatctttt tttgttggaa ctttgtattg taatagatta
     4261 gaagtttgaa gctttccatc agaaccatgt ctagggtctt catacattgc aagccctata
     4321 ccttgagtta caccaccttc aacttgtatt ctagcaagtg caggattcat aactgtacca
     4381 caatctgtag aacaagcata atttattact ttaaactccc ctgttgtttt atctaactct
     4441 atttcagcaa atcctgctaa gaatggttta gcactctctt gtataccata agattcagat
     4501 gaatataaaa cttcttgatt tccaccacca acagattctt ttcctaattc acttaacaaa
     4561 acaaacttct ctacatcttc actataacaa actctgtctt catatagtat taaatcatct
     4621 attggtaaat cgagtttctt ctgtgctact tttaatatct tttgttttaa actttcagca
     4681 gattttattg tagcatttcc agttacataa gtagtacaag aagcgtatgc accagtatca
     4741 tatggacaca tatctgtatc tcctgtataa acggaaattc tatcaataga tgtattcaat
     4801 gcttttgcag ctatttgtgc aagtattgtg tcagaaccag ttcctaagtc tgaagaccca
     4861 gaaaacaact tataagttcc atcttcatcc atcctcatat ttactattgc catgtctatg
     4921 ttggctatac cagaaccatg agttcccaca gacattccaa cagccctcac cttattgttt
     4981 ccaatatccc taacagggta cttttcatcc catccaataa gttttttacc tctaattata
     5041 cattcatcaa gtgcacaact tcttattgga aaattcatta ttccgccttc agtttgtttt
     5101 tttataatat tctttaactt tatttctgtt gggtccatat taagtttatg cgctaattca
     5161 tcaattgcac aatcaagtgc aaatgtacct tgtgttgctc catatcctct taatgctcct
     5221 cctggaacta aattagtata tacagttctc ccatcaaatt ttattgcagg tacattatta
     5281 tatgttggaa gtacattatg acctgattca gagcaaactg aaggtccatt atcgccataa
     5341 gcgcctgtat tgttcaatgc tttcatatct atagctttta tgtttccttc tttatctgac
     5401 ccaattttta tatcaaaaaa catctgatgt ctagtattag tcattgcaaa agtttcttct
     5461 ctagtataaa ttagttttgc tggtcttttt gtcatccaag ttacaaatga tgcatatatc
     5521 tctgttacag ctatgttttt tccaccaaaa cctccaccta ttcttggttt tattactctc
     5581 acttttgaaa gaggtattcc cagtgcttta gaaacctgtc ttctcatatg ataagctgat
     5641 tggtttgcag aagttactac taatctgcca ttcgtatcta tatacgtata agctctatga
     5701 gtttccatca tacaatgggc ttgtgattga gtcatataag aatgttctac tattacatca
     5761 ctttctttaa actctttttc tacatcccct ttacccattt tttctctcga tacaatattt
     5821 tttgtagcat ccaatccaaa atcaaatgga caaaacaaat catcttcttc atggattaaa
     5881 attttatttc cttccgattc tcttgcatcc aatataggag tcattacttc atattcaact
     5941 tttataagtt tcatagcttt aatagcagcc ctttcatctt cagcggctat tatagcaact
     6001 tcatctccta catatctaac tacgtcttca agtattaatt ggtcatgagg tgatgcttct
     6061 ggatatgact ctccaaccat tgaatatcta taatttggaa catctttgta agtgaatatg
     6121 tttactacac cttttacctt taatgcattt tttatatcta tgttttttat cttcgcaaaa
     6181 gcatgtggac ttctaagaag cttaattgtt aaaacatctt tatgaaaaat taaatcttct
     6241 gtatatattg gttttccagt aacaatggct tcactatcta tttttcttac ctctttattt
     6301 accatcacac tcacctccaa tactattata cattctcact actaaggctt ttgccatatc
     6361 ttttctgtat tctttacttc ctcttatatt acttccaaat gaaagttctt cacttgatac
     6421 ttcaccagct ttttctatat cattttctaa acttaatatt tcacttgctt tataggcaat
     6481 ttttgctctt cttggtctag cgcctatcgc aattttataa atacttcctt cttttaacat
     6541 tgctccattt aatactgcaa aatcacctgt acattttctt atagaatcaa atacacctat
     6601 agcatctttt ttaggtaata taacttctat taatatatct ttttcaagct cattttctaa
     6661 aaaatcagct aagttcataa cccctttttt atataactta acctttgcat ctaccactag
     6721 taatgaaggt attaaatctg aaaatccata cttagcaaat acgctagctc ctactctagc
     6781 tccacttcta aattgaactc ctactatatt agaaacagcg tttgaaatta ccccattaca
     6841 gtaattttta ataaccttat ttaattctaa agttcttaaa cttgtatctg ctccaattaa
     6901 tatgctatca tcagtttcat ttatataatc taaagctaag tcttttaaat ctattgctga
     6961 accaatattt ttactaccta gttttaaaaa tccacatcct ccaagtatta tgttgttttt
     7021 tcttgatatt agcagttcat aagcttcatc taaacttttt ggaactacat attcctttat
     7081 agttatcata ctctctcccc atatcaactt aattttcact taaaagtcta agtgtttaaa
     7141 tatttatgta ttgctcttaa ttgaccttta tatcctgtac atctacaaag gttaccattt
     7201 acataatcta taacttcttc ttctgttgga ttttgaagct ctcttttcat tgccaagacc
     7261 atcattataa atccaggagc acaaaatcca cactggtcag ccccttcgtc tgcaagaaaa
     7321 tctccaaact tctttgcttc atccaagaca ccctcaagtg ttgtaatatg ctgaccttct
     7381 actctagcag taagataatt acatgaaagc acggccttgt catttaccat cactgtacat
     7441 aacccacatg agcctgtatc acaccctttt ttaacactta catatccatt ttctcttaat
     7501 gtatctgcta aaaaatcatc tggtcttata cttaattctt tttctttatt atttatggta
     7561 catttgataa tcaaataaat cacctcaaaa ctcatatttt taagctaaag ttttactata
     7621 cttttttatg aataaattac atttattaat aaaggctatt ccatttatga ccttccattc
     7681 tattttttat agttcctctt acacaaagtc tctcagctgt atcttgagca tcttttaatg
     7741 ctttctcttc attagctgtc aatatttttg aatcttccat tattatattt ccatctacta
     7801 taacactctc tatatttttc atactagaag aatatactaa tgtagatacc ggattatgca
     7861 taggtatcgc ttttggtgaa agcattggat taaatattaa taagtctgct tttttaccta
     7921 tctccaatga acctatctca tcttccattc caatagctct tgctccatct atagttgcca
     7981 actcaagaac tttttctgct gacattgcta atgggtcaca cttattaact ttatgttgaa
     8041 gtgctgttaa tttcataagt tcaagcatat cttgagagtt attactagcg gctccatcta
     8101 ctcctaaact aactgttatt ccttttttaa gcatttctgg cactggtgca acacctgaag
     8161 ataagtacat attacttgct gtattatgag acactttcat atcgtatttt ttagtaagtt
     8221 ccatatcttt ttcagttaga tacacacaat gtaccataag cacatttggt ccaagtattc
     8281 ctaatttttc taaaacatct atgtcatatt gcccatgcaa ttcttttgct gcttctctat
     8341 caaaaggagt ctctgatata tgtactgtaa ataaagcatc atcatattct tttactactc
     8401 tccatagcat ttcaagcatt tcttgagaat ttgaccatat tgctgcagga gctactccta
     8461 ttttgattct tccattttct gtattatggt gtttttcaaa caatcttctt acatcttttt
     8521 caaccgtttc tacatcttgc attattccag gatgaactcc aaattgagct cctgtattca
     8581 tacatcctct tccaagtatt cctcttattc ctaattcttt ataagcatct attattccat
     8641 cacaaagacc tggcttacta tgaggataca tgtagtcaac cattgttgtt ataccacttc
     8701 taagtccttc aatacatcca agcattgcag catcataagt atcttttggc tctaaatagt
     8761 tagctgctgg aaatgtcata gtttctaacc agtctttaag taccatatca tctcccaatc
     8821 cttttaataa agtttggaat aaatgattat gtgtatttat aaaacctggg aataaaactt
     8881 tacctttagc atctataatt tttttaacat cagtatattt actttctatc tctttagaat
     8941 ttccaatatc agcaatcttg ttatctttta ctactaatgc cccatcaaaa ataacttctc
     9001 tctctttatt cacagttact attatggcat tcttaataag tatatccatc attcttattt
     9061 ccccccgcat tatttagtaa tttttctttt aatatattga cctgttggat gttctttaac
     9121 attacaactt tgtactacat gatttcctct caaaaataca tcttcaactt tacatttaac
     9181 cttaaatcct tcatatggag tataatctac attttgtttt tgagttttat aactaattgt
     9241 ttcttcttta ttgatattta aaatgactat atctgcatca cttcctacag ctatctcacc
     9301 ttttttagga tacattccaa atatttttgc agcattcgta gaagtaactt ccacaaactt
     9361 atttgcactt attctatttt ctaagactcc atatgtatat aaaagtgcta acctatgttc
     9421 aacaccaggt gctccatttg gtatcttact aaaatcatca attcccaagt ctttttgccc
     9481 cttaaaatta aatgaacagt gatctgtgcc aactgtttga atatctccac tagacaatcc
     9541 ttcccaaagg taatttatgt cttctttttt tcttaaagga ggagacatta catacttagc
     9601 accctcaaat ttatccttat tatataaatt gtcttctaat aatagatact gtgggcatgt
     9661 ttctactatc atatctatat ttttttctct agccattttt acagtttcaa gtgactccct
     9721 acaacttaag tggactacat aggattttga attagataat ttagttatat cagcaagtct
     9781 tgaaacagac tctttctcaa ctatgttagg tcttgtaagt ggatgatatt ttggctccaa
     9841 attaccattt tgtatatttt cttctatcaa tacatctagt aaatccccat tttcacaatg
     9901 aaatccaact atacaaccta aatcccttgc tttctttaaa accctgtata ttgttccatc
     9961 atctactttc atgccatcat atgccatata catcttaaaa gatactatcc catccgaaat
    10021 aagtttttcc atatgattaa atgtatcttt atttaaacat gtaattgtca tatgaaaacc
    10081 ataatcacaa taacaatttc cacttgcttt tttatgatat gcttctacac cttgcagtag
    10141 gttttccccc tcattggctt ctgcaaaatc tagtattgta gtagttcctc cagctatagc
    10201 tgctcttgtt cctgtttcaa aattatctgc agttgtaata gaacctgcat tcatatcaaa
    10261 atgagtatgt gtgtctattc caccaggaat tacaatttta cctgtagcat caataacctt
    10321 atcaccattt atttctaagt tatttcctat ttcaactatc tcttcatctt ctatccttaa
    10381 atcacttata taagtcttat cagaggtaat tatagttcca cctcttagta ttgttcccat
    10441 atatacctcc taacttttaa gctttaaagt attttctaag tattgtaaaa ctttatttaa
    10501 aattgtattt tctaaaggtg ctatattcag cacctctaga ctatatttaa ataatttatt
    10561 gtgaactaaa tattattttc cgtatacaga ttttaatatt ccatagtaac cttctgttcc
    10621 tataaataac tgttcttgct ctatatactc atctattgtg tgagcaagat tttccttaga
    10681 aggaccaaat cctatagttt ttattccagc ttcacctgca tagtgacttc cattcgtaca
    10741 gaatgagtat tgagttattt ctgaatctat tccagcttct tttaatccct tatatgctgc
    10801 ttgaacaaat tcatcttctt c
//
LOCUS       DAMJVI010000113        10785 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.118, whole genome shotgun sequence.
ACCESSION   DAMJVI010000113 DAMJVI010000000
VERSION     DAMJVI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.118"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            25..792
                     /locus_tag="PC545_003089"
     CDS             25..792
                     /locus_tag="PC545_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HDF2457832.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKAWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPITADNYIFINCFWISGKFKGQGYSNLLLQECVDD
                     AKSKGKYGLVVLSSSKKMPFLSDAKYLKYKDFCLADTSEPNYELLYFPFTDNAPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYAPILKKIANSRGIDVNLIKFETAKEAQNAP
                     APFTTYSLFFNGEFVTNEILSEKKIEKFLDTNIKKFN"
     misc_binding    1010..1223
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1477..2511
                     /locus_tag="PC545_003090"
     CDS             1477..2511
                     /locus_tag="PC545_003090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDF2457833.1"
                     /translation="MNKIKVGIIGATGYVGAELIRLLMNHDKVEVTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDMCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKEDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKNNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCKEKVSVVFTPNLIPVNRGILSTIYCTKEDNISINDIHRKLTDYYEFKEFIEIL
                     PLDKIASLKNVRFSNKCVISLHENGDTLIICSAIDNMIKGAAGQAIQNMNIIFGIEEN
                     TGLKNIAPSF"
     gene            2552..3769
                     /gene="argJ"
                     /locus_tag="PC545_003091"
     CDS             2552..3769
                     /gene="argJ"
                     /locus_tag="PC545_003091"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HDF2457834.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     NIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEAHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            3784..4644
                     /gene="argB"
                     /locus_tag="PC545_003092"
     CDS             3784..4644
                     /gene="argB"
                     /locus_tag="PC545_003092"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDF2457835.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFEEGIITGGMIPKIEGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            4674..5861
                     /locus_tag="PC545_003093"
     CDS             4674..5861
                     /locus_tag="PC545_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDF2457836.1"
                     /translation="MNNDNTISKWNEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYSGEKIINSSGMAKVYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYVERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLEIVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKTKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYKEIDEALEILKDILL
                     EIN"
     gene            5970..6992
                     /gene="argF"
                     /locus_tag="PC545_003094"
     CDS             5970..6992
                     /gene="argF"
                     /locus_tag="PC545_003094"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDF2457837.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVSDTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGDIMLTE
                     NIDDVKGADVIYTDVWVSMGEEEQYEARINQLKDYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTNEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            7119..7736
                     /locus_tag="PC545_003095"
     CDS             7119..7736
                     /locus_tag="PC545_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HDF2457838.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTIDSFFNSAKKFDFEGM
                     NKVMENNDEKYKDILKELDTKDPNAQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMIGMSFSGQDLTDEKTTEMLVSIMKEKQKSVKETYVTK
                     NVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSMNTLE"
     gene            complement(7956..8654)
                     /locus_tag="PC545_003096"
     CDS             complement(7956..8654)
                     /locus_tag="PC545_003096"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDF2457839.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSLPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKESINI
                     PILNIVEETVKKLPTISQKVTLFSTSSTFESTIYQNGIIHNGHEFIFKDEWQIKLNNL
                     IQNIKMDKENPHNIDIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(8816..10033)
                     /locus_tag="PC545_003097"
     CDS             complement(8816..10033)
                     /locus_tag="PC545_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HDF2457840.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWME
                     GLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNRSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TKFGFNTKNLTNLHPRKDVDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPERA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(10224..10637)
                     /locus_tag="PC545_003098"
     CDS             complement(10224..10637)
                     /locus_tag="PC545_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457841.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         4212 a         1183 c         1805 g         3585 t
ORIGIN      
        1 atgcaattag aaaaggagat aagtatgcag attattgata taaatataaa taatattgac
       61 aatgaacata tatgttgttc aatttctgaa aaaaaaggag aaaattgtgt tttttctaaa
      121 aaagcttgga tgaaaaataa ttttgataaa ggtctagtct ttaaacgatt tgatgtaaga
      181 ggtaaagctt ttatagaata tattccagca gaaaatgcat ggtgtcctat tactgcggac
      241 aattatatat ttattaactg tttttggata tctggaaaat ttaagggtca aggatattct
      301 aatctattgc tacaagagtg tgttgatgat gcaaaatcaa aaggtaaata tggattagtt
      361 gtgctttcgt ctagtaaaaa aatgcctttt ttatctgatg ctaaatatct aaaatataag
      421 gacttttgtt tagcagatac atcagaacca aattatgagc tattatattt tccctttact
      481 gataatgctc ctattccatg ttttaagaat tgtgtaaaac aaagaaaatt aaatgagaat
      541 ggtatagttt tatattacag taatcagtgt ccacatacgg aaaaatatgc tcctattttg
      601 aaaaaaattg caaattctag aggtattgat gtaaatctta taaaattcga aacagcaaaa
      661 gaagcacaaa atgctcctgc accatttact acatacagtc ttttttttaa tggtgaattt
      721 gtaaccaacg agatactttc tgagaaaaag attgagaaat ttttagatac caacattaaa
      781 aaatttaatt aaaaagagat gtaaatattg tattttgaaa atatattatt ttataattta
      841 atatattttg gatgtttttt aaattgacga atgaaatata aaaattatat cgcaatttag
      901 atacaaaatg ctggaaatta accatataga aaatatttcc aaaaatttaa aaattatatt
      961 gacaaagtta aaaaaataaa ctatcatata aaacaataag aaaataaatt gaaatcctgt
     1021 gaaaaagaga gtagcataaa gctagttgat agagagctgg gaatggtgga agcctagtaa
     1081 cgaattttat tgtgaataga gctttggagg agattaccag aagtaatagt atggtaattg
     1141 gtgaatctcg ccttaagaga ttagagtggg taagattatc ttatcaatta gagtggtaac
     1201 gcgggtgtat attcgtctct tggtctttga ccaagaggct tttttaattt taaatgtaca
     1261 ttttgtagat actttttaat ttgagtttta atgattattg taattttacc aataagagtg
     1321 gtaccgcgga aaatcgcctc ttagtattta taatgctaag gggttttttg ttttaattag
     1381 agtattttaa tctacgtatt atcaaatgat tatcatatag taaagtggaa agataggatt
     1441 ttaagactat ttgaatataa aaatggaggg aacaaaatga ataaaataaa agttggtatt
     1501 attggtgcaa ctggatatgt cggggcagag ttgattagat tattaatgaa tcatgataaa
     1561 gtcgaagtga cagcgatagg ttcaaattcg tatgttggaa aagatatagt tgatatatat
     1621 ccaagtatag gatataaaaa taacatgata tgtatagaaa atgaaaaagt tatagatatg
     1681 tgtgatgttg tgtttacagc ccttccacat ggagttagtg aaaagtttgt aataaaggca
     1741 attaaatcga aaaagaaagt tatagattta ggtgctgact ttagaataaa agatgaagaa
     1801 gtatatagta aatggtatgg agtatctttt atagataaaa tcttacataa aaaagctgta
     1861 tatggattaa gtgaaatata caaagaagat ataaaagatg cagatataat tgcaaatcct
     1921 ggatgttatc caactagtat ttctttacca ttgatgccat tactaagttc taaattgata
     1981 aaaaataata atataataat tgattctaaa tcaggtctta caggtgctgg tagagaatta
     2041 tcgattagta gtcactttac ggaggttaat gaaaatatca ctgcatataa aatagggaaa
     2101 cacagacata ctccagaaat agaacaaaat ttaagtgaat cctgcaaaga aaaagtaagt
     2161 gtagttttta caccaaactt aatcccagta aataggggaa tcttatcaac tatatattgt
     2221 acaaaagaag ataatatttc tataaatgat atacatagga aattaactga ttattatgaa
     2281 tttaaagaat ttatagaaat tcttccatta gataagatag cttctttaaa gaatgttaga
     2341 ttctcaaata aatgtgtaat atcattacat gaaaatggag atacattgat aatctgttca
     2401 gctatagata atatgattaa gggagcagca ggtcaagcta tacaaaacat gaatataata
     2461 tttggtattg aagaaaatac aggtttaaaa aatattgcac catcatttta gaaataaaaa
     2521 atactaaact catataatta ggaggataaa tatggagatt ataaagggtg gagttacagt
     2581 ttcagaagga ttttttgctt cagggattca ctgtggcttg agaaaaaata aagaaaaaag
     2641 agatttagct ttggtttatt cagatgtttt atgtgatgca gcagcagtag taactcaaaa
     2701 taaagtaaag ggcaatcctg tttatgttac acaggaacac attaaaaatg gaaaagcaca
     2761 agctatcata gtaaatagtg ctaatgctaa tacctttaat ggaaaagaag gacttataaa
     2821 tgcttataaa atggctaaat tcaccagtga taaattaaat attaaagaaa gtgatgtttt
     2881 ggttgcatct acaggtgtta taggaaagcc attaaatata gatttaatag aagaaaatat
     2941 ggatgaatta gtgaataatc taagtaaaca aggtcatatt ggagcaagag aagctattat
     3001 gacaactgat attataaaaa aagaaatagc agtagcaata atgtatggag ataaaaaaat
     3061 aactattgga ggtatggcaa aaggttctgg tatgatacat cctaatatgg caacaacgtt
     3121 aggatttata actacagatg caaatattga tggagttttg ctaaaagaag ctttaaaaat
     3181 tgctgttgac aaaagtttta atagagttag tgtagatgga gatacaagta ctaatgatat
     3241 ggtaatgatt ctagcaaatg gaaaagcaaa aaatgataga ataaacaaaa aagatgaaca
     3301 ttatcaagta tttttgagtg cacttacata tgtctgtata gaacttgcta aacttgttgc
     3361 aaaagatgga gaaggatgta caaaactaat tgaatgctat ataaatggag cattaagtga
     3421 agcacatgca gtaaaattag caaaaacagt tatatcttct tcattagtaa agacagctgt
     3481 atttggagct gacgcaaatt ggggtagaat actgtgtgct ttaggttatg caggtgaaga
     3541 aatagatatg gaaaaagttg atattatttt tgagagtatg aaaggatata ttgaagtatg
     3601 taaaaatgga aatggattag attttaatga ggaaaaagca aaaaaaatcc ttgaagatga
     3661 cgaaataagt atattagtag atttaaatat gggcaatgcc agaggaaatg catggggatg
     3721 tgatttaagc tatgattatg taagaataaa tggaagttat agaaattagg aggatatact
     3781 ttaatgatta atatagaaaa agcaaataca cttatagaag ctttaccata tatagaaaaa
     3841 catcagggaa agactattgt agttaagtat ggtggaagtg caatgaaaaa agatggtcta
     3901 aaagaatctg ttatggaaga ccttgtgtta atgagttatg ttggaatcaa tatagtttta
     3961 gtacatggtg gaggggcaga aatcaataag atgctagcaa aagtggatat agaaagtaaa
     4021 tttgtaaatg gtcttagata tacagatgaa gagaccatgg aaattgtaaa aatggtactt
     4081 gctggaaagg ttaataaaga cctagtaaat aaaatacata caaaaggtgg aaaagcagta
     4141 gggctttgtg gaatagataa taatatgatt ttatgtgacc catataaaaa ttatgaactt
     4201 ggatttgtag gagaaatcaa aaaagtaaat gttgagctta tagaatcttg tctaaagtca
     4261 ggttatattt cagtaattgc aactataggt gttggagatg atggagagac ttataatata
     4321 aatggtgaca cagcagcttc tgctatagct aaagagttaa atgcagataa attgatactt
     4381 cttacagatg tccctggttt gttaagagag ccagatgaag aaaaatcatt aataacagaa
     4441 gttatattag aggatgttga taaattattt gaagaaggta taattactgg aggtatgata
     4501 cctaaaatag agggatgcgt agatgcctta aataacggag taaatagggt acatattttg
     4561 gatggaagag ttccacattc tataataaca gagctattta ctgatagtgg aataggtaca
     4621 ttaataagaa aagaaaatga ataaaattat aagtttaaga ggggagaata agaatgaata
     4681 acgacaatac aatttcaaaa tggaatgaat attttataga tacatataat caacccaatt
     4741 ttgtcataga ttatggtgaa ggaagttgtt tttttgatac taatggcaat aaatatatag
     4801 attttacaag tggatatgga gtatcttctc ttgggtatag taatagtaat ttaaagaatg
     4861 ctctaaaaga gcaagtagat aaattattac atacttcaaa tttatatttt aatgagccag
     4921 tgttgtattc aggagagaaa ataattaatt caagtggaat ggctaaagta tatttttgta
     4981 attctggaac agaagcaaat gaaacagcat tcaaaatagc aagaaagtat agtagtgaca
     5041 aatatggtaa tggtagagga actatcataa gtttaaaaga ctcttttcat ggtagaacaa
     5101 tgatgtcttt aatggcaact ggtatggaca aataccataa gtatttttat cctcttcccg
     5161 aaggttttaa atatgtagaa agaaataata ttgaagattt aaaaaataat cttgattcca
     5221 cagtttgtgc aataattctt gaagcgattc aaggcgaagg tggagttaat gttcttgaaa
     5281 aagattatgt tttggaaata gtaaaaatat gtcaagaaaa ggatattgta gtgatttttg
     5341 atgaagtcca atgcggtata ggaagaactg gtaaactttt tggctatgaa tattttgatg
     5401 taaaaccaga tatagttacc gtagcaaaag ggttgggagc aggaatacca gtaggaggag
     5461 tattagtaaa taaaaaactt tcaaaagtac taggaaaagg agaccaaggt acaacttttg
     5521 gaggtaattt attagctatg gtggcagctt cagttgtatt agatgagata tctaaagatg
     5581 gtttttataa tgaagtatta gaaaaaggaa attatataag aaaatctatt gaaagtttta
     5641 ataacaaagt tgtattaaag acaaaaggta ttggtctaat gataggaatt gaaactaata
     5701 ttgaatctag tattatagaa gagaaagcta gaaaaaaagg attgcttatt ttgactgctg
     5761 gaaaaaatgt attgagattt ttaccaccat taacaatcag ttataaggaa atagatgagg
     5821 ctcttgaaat cctaaaagat atattactag aaattaacta aatattcaag aaatagttga
     5881 ttaaagataa aaattagaag taaataaaaa agtaaagtgc taacaagttt aattgaattt
     5941 aaaaagttta taagaagggg cgaattaata tgaataatat aagtttaaaa ggtaagagtt
     6001 ttttgacatt aaaggatttt acaaaagaag agattagata tttattaact ttatcaagtt
     6061 atttaaaaac taagaaaaaa gttggtataa aaggcgattt gctacaagga aaaaatatag
     6121 ctttattatt tgagaaaaca tcaacaagaa ctagatgttc atttgaagta gcagctcatg
     6181 atgaaggagc acatgtaact tatctaggtc caaatgattg tcatatgggt aaaaaagagt
     6241 ctgtatcaga tacagcaaaa gttctaggaa gattctatga tggaatagaa tttagaggct
     6301 ttaaacaaga aacagttgaa agtctagcaa aatattctgg tgtgcctgtt tggaatggac
     6361 ttacagatga atatcatcca acacaaatac tggcagattt tttaacagta attgaaaatg
     6421 tagataaaga tttgaataaa gttaaatttg tatatgttgg tgatgctaga aataatatgg
     6481 gaaactcact gatgatagga tgtgctaaaa tgggaataca tttcgtagca ctagcaccta
     6541 aggaactttg gccaaatgaa gaattggtta aggaaatgaa ggaaatagcc aagaaatcca
     6601 aaggagatat aatgttgact gaaaatattg atgatgttaa aggtgcagat gttatatata
     6661 cagatgtatg ggtttctatg ggagaagagg agcaatatga agcaagaata aatcaactta
     6721 aagattatca agtaaatatg gatatgataa agaaaactga aaatgaaaat gttatatttt
     6781 tacactgtct tccagcattt catgatttag aaactaaggt tgctcaggag atttatgaaa
     6841 agttcggatt atcagaatta gaggttacaa acgaggtatt taattcaaaa tattcaaagg
     6901 tatttgaaga agctgaaaat agaatgcata caataaaagc tgttatggtt tctactatga
     6961 ctgatgtaaa tatgaacaat aatttaatat agatatgttt taatatagac tttactttaa
     7021 tgaaaattta taaaatattt taaatatatt attcaaaaac tctcattata tgttaaaata
     7081 acttatgtat caacaaatta agagggagga tttgagatat gaaaaaaatc atattagctt
     7141 taacaacttt tttattagca atatctttaa cagcatgttc aagtgcaaaa ccagaagata
     7201 caattgatag tttttttaac agtgcgaaaa agtttgactt tgaaggcatg aataaggtaa
     7261 tggaaaataa tgacgaaaaa tataaggata tattaaagga attagataca aaagacccaa
     7321 atgcccaata tgttttagat tatctaaaac agaatgcatc taaaatcaca tatacaataa
     7381 aagacagcga ggttaaaggt gataaggcta caatcaaagt agaatgtaaa tttatagatt
     7441 caacaccatt acttcaagaa atagtagctg aggcatttac aaagatgatt ggtatgagtt
     7501 tttcaggaca agacttaact gatgagaaaa caactgaaat gttagtatca attatgaaag
     7561 aaaaacaaaa aagcgttaaa gagacttatg ttacaaaaaa tgtagagttt gaatgttcta
     7621 aaaaggataa taaatggata ataagttcag taaatgatgc agtagctgat gtattgttat
     7681 ctaatcttgt tacagcagga caagaatttt ctaactctat gaatacactt gaataatatt
     7741 gtttaggtgt agtaaagtaa taaatatatg attattttta taaataaaca tataaatatt
     7801 tatgaaggat accatttttg gtatcctttt tattaatata atgaaataca tggttaattt
     7861 ctatattgtc aagaaactat gatatatgtt ataagtctat actatttaaa ataaacttat
     7921 aacatatatt ttttataata tagtgcatga aaaatttatt taactaattt tagatattta
     7981 ttgacaactg attcagccaa gcattttgat gaatcaacaa tatttattga aggatatgat
     8041 ttttctaaaa ctacatttaa atcagtacaa gcaattacta tattttctat agattcattt
     8101 ttaacatctt cgataagttt attccaaata tcaatattgt gtggattttc tttatccatt
     8161 tttatatttt gaataagatt gtttaatttt atttgccatt catctttaaa tataaattca
     8221 tgtccattat gtattatacc attctggtaa attgtagact caaaagttga actagtcgaa
     8281 aaaagagtta ctttttgtga gatagtaggt aattttttaa ctgtttcttc tacaatattt
     8341 aatattggta tattaataga ctcttttaat tctttaaagt atatatgtgc tgaattgcat
     8401 ggcattgcaa taaaatttac accagttgat tcaagctttt gtaatccatc aattatagtt
     8461 tctttcataa gttcatgatt tattggacgg tcaatataaa atggggtagg taatgaatat
     8521 atcatcatct ttggaaattc atcatcatat tttgctccat attgagattg acactcatca
     8581 ataattaaat caacaaaagg ggctgttgac cttggaccca ttccagccaa aataccaatt
     8641 aatttttcat tcataaaaat tatctccttt atatataaat atatttatgt ataaaataaa
     8701 aatctataaa agcataaaat tatattttat aatagattat attgtacact tatataatat
     8761 aaatataaag tgatttttta gattatatat tttaatatac tttcttatat aagaattatt
     8821 ttaaatcaag attatctagt atttttaaaa atatttttac agcagtatcc aagtcatcta
     8881 cagaaacact ttcattagga ttatgactta cgcccccagc gcatcttata aataccatac
     8941 caatatcagt tatattatcc atttcctgag catcatggcc agcaccacta taaatataaa
     9001 aaggattaag atttaaatct ataaaacttt tttctatgat ttttgttatt ttattagaac
     9061 atggtacagg aacattttca aaagcaagtt cacttgtata attaagtttt ctattttcac
     9121 atatatgtga tatttcatta aatatctttt ctacagaatt atcaagaatt tcttgtgaac
     9181 aagagcgtac atcaagagta aattcagctc tttcaggtac cacatttgat gaagatggat
     9241 agaaattcat ctttcctaca gtagcaacca aatcagcagt cgttttagct actttttcga
     9301 tagctaatac acattcacag gcacaacatc cagcatcaag tctcatattc attggaagtg
     9361 taccagcatg tccagactta ccatttactg atatcttaaa acttttaaat cctgtaatac
     9421 ttgatacaat tccagcagga agagattcat tttctaaaac aggaccctgc tcaatatgaa
     9481 attctaaata tgcatctaca tcttttctag gatgcaaatt tgttaagttt tttgtattaa
     9541 atccaaactt tgttacagct tcttcaagtg taattccatt ttcatcaaca gatttaagaa
     9601 gttcctcttt aaaagtacca gcaaaaactt tacttcccat taaagaagtt tcaaatctag
     9661 ttccttcctc ttcagcgaaa gcaattagct taatattgaa aggataagac ctattattgt
     9721 gttttgcttc ttcaagacca actagaggca ctattatacc taacatacca tcaaatatac
     9781 ctccacactt tacactatct tgatgagatg caactactaa tgtgggagca tttggaaagc
     9841 ttgatttata tgtacctata acgtttccaa tgtcatcaat ttcaatatca agatttaaac
     9901 cttccatcca tgaactaatt aaatcaatag attttttatg ttcttgagtc aagataagtc
     9961 ttgtaagacc atttgttgtt gaacttattt ttcctaatgt ttgtagataa tccatacatt
    10021 tttttcctaa catatttacc tcctaaaaat ataattttta tttaagctaa gattagcttt
    10081 tatacttata atttttgaac tttcaaataa ttttgtatgt atagtaaaat actattttag
    10141 tattaataat gatataaaaa tataaagtct gtagacgaag agcagaagtt ttaagttaaa
    10201 aatataaaat atgattttta ttattatagt atttttctaa aataatatct ctttccagtt
    10261 agaggataat tttcaagtga aaatacttta tggtatccaa atttctcata aaatttaggt
    10321 gcttgaaaac tgaatgtatc taaaaaaaca tatttacaac ctcgttcttt agcaattttt
    10381 tcagccttat ataaaagttg acttccagta cctttatatc taagcttctc actaacccat
    10441 aagtatttta tagaaagcca attaccatga gtaacaccta taaaacctgc aacaatttta
    10501 cctgctttat ctcgaatata tattcctaaa tccactgggt ttttatcttc aagtctagca
    10561 agattatatt ttaataatcc ttgaaaaata gtatcttcat cttgtttttc tattttttct
    10621 gtaatttcta attccatatt tgaactttct ccttgtataa tcttttgtat attggtattt
    10681 tttaataaaa tcagtagaaa gaatatagtt taacattatt ctaataggca tttatgctaa
    10741 tgtcaaacta ataatactta ttcttaattt tatattgttg atatt
//
LOCUS       DAMJVI010000114        10775 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.119, whole genome shotgun sequence.
ACCESSION   DAMJVI010000114 DAMJVI010000000
VERSION     DAMJVI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10775
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.119"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(150..482)
                     /locus_tag="PC545_003099"
     CDS             complement(150..482)
                     /locus_tag="PC545_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDF2457842.1"
                     /translation="MNTREMKEYRGFYNKKLSETDEFFRVFGSLDDKTYSEGAISKKN
                     KELMGLAISILSRCDECICYHIEGCINSGASKDEIIEAIKIGVIGGGSITYPNARFAI
                     KILEEFNI"
     gene            complement(726..1367)
                     /locus_tag="PC545_003100"
     CDS             complement(726..1367)
                     /locus_tag="PC545_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDF2457843.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVIIQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKRNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFVYSYLMYKISGKNN"
     gene            complement(1348..1641)
                     /locus_tag="PC545_003101"
     CDS             complement(1348..1641)
                     /locus_tag="PC545_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2457844.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            2034..2084
                     /locus_tag="PC545_003102"
     CDS             2034..2084
                     /locus_tag="PC545_003102"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457845.1"
                     /translation="MTEYIYYYNNKRIKKN"
     gene            complement(2610..3362)
                     /locus_tag="PC545_003103"
     CDS             complement(2610..3362)
                     /locus_tag="PC545_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDF2457846.1"
                     /translation="MFSVIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKINNNLLKKLVPFTIIGSVLGVRCVLSISEEILNVLV
                     VAMILIVAIYTFISKNLGQEDNFEAVNKKNLRLGMLMALIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYVGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(3421..5076)
                     /locus_tag="PC545_003104"
     CDS             complement(3421..5076)
                     /locus_tag="PC545_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2457847.1"
                     /translation="MVDFIESNNEVQKFDWGEVMWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKVNIKETSIVYCPPYSEHEIINTGKIDLVI
                     LITYVPHKFSSLRQIPIVFSENNIQELVNVNIIENLANQISNILKLRISVYDLKYKEI
                     FETKKENKFCDICKNIKPCTKKLVKSINNDNFEKVYHCEYGVSSLEIPIVLEENIVGY
                     IECGNFIVYKSIYIDKNLSELSSSSGIDIKYINKIYNEFPLNPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKSRKENIRLEEALKKANSRLLEEEYIINPIDMFN
                     NSSKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLRYNDVRDTIQEMIFVLSRTVLRD
                     LEDLKLISYLRNKYNKKISLVNSDEDLWNVLFEFSKECIDKNRAFWRKDKGNLIENIN
                     EYIKKYYKENINLNSISDVFFISPNYLSSIFNERNKVSITEYINLLRIEESKKYLLDR
                     SMSVSDICKKVGFNNSSYFSQIFKKFNSITPNEYRKNMLDNKY"
     gene            complement(6348..7427)
                     /locus_tag="PC545_003105"
     CDS             complement(6348..7427)
                     /locus_tag="PC545_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2457848.1"
                     /translation="MILITLCILVAVFVLAFAGLRLIAPTIIRTSHKIEAPGIDRMEM
                     VEIGGIQQALYIRGQNADNPIILWVHGGPGNSMMPFLHLYQYEWENDFTVVNWDQRNV
                     GKTYFANDPAAVLQTMSAERVLQDVHEVTAYLKQEFNKEKIILIGHSWGTVLGTMAVQ
                     TYPEDYSAYIGIAQTVNMVDNERVGYEKTLEMVKAAGKQKDIKALEAIAPYPPAKYGP
                     SYLAALMTVRTYQSKYGLSINMDLGNIISILATPYYSLRELKNTAIVNPNAIDNQGDV
                     LRFLVEEADIRNYGVDYQVPVYYIMGENDYNSPYLPAKKFFDEIVAPDKQIFFIPDAD
                     HLPFLSKKAEFDCVLLEEIAPKLAK"
     gene            complement(7511..8167)
                     /locus_tag="PC545_003106"
     CDS             complement(7511..8167)
                     /locus_tag="PC545_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2457849.1"
                     /translation="MTRKNNPKQAIENIITISAKLFAEKGYDKTSMQNIVDASGMSKG
                     GIFHHFSSKEDIFNVVMERRFEQIIETVNQWLGEMHGLTAKEKLRSLVRRHLTDEVIK
                     ESSNMITSAIESPHIILAFTQDNLKKLAPILANVLREGIEDRSIITEFPDECAEVILL
                     LFNFWCDTDVFQGDFPTLRKRFQFLQFLMRQIGVDILEDEIIESINIFYERKPMGQVE
                     "
     gene            8489..8695
                     /locus_tag="PC545_003107"
     CDS             8489..8695
                     /locus_tag="PC545_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457850.1"
                     /translation="MISHAAEICKKYKMPFIIFPTIEKKSIEEWVNIGANMLCFAQDT
                     TFITYVINQLLSFYNEIVPIQKQH"
     gene            complement(8848..9276)
                     /locus_tag="PC545_003108"
     CDS             complement(8848..9276)
                     /locus_tag="PC545_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2457851.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFVAEGVKLKGKTPEVLDVSLLKPS
                     DLKEEDKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMRDW
                     EERMQNAGATIIGGEGITAMEDPNEEAKDECIELGKTLAE"
     gene            complement(9752..10690)
                     /locus_tag="PC545_003109"
     CDS             complement(9752..10690)
                     /locus_tag="PC545_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HDF2457852.1"
                     /translation="MNKRMKLIPYEINENLRGAKNKFPYGIKQMNARGMWDEGYTGKN
                     IVVGIIDTGCDISHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVMQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMDNKLAIMSGTSMSAPYVSGSLALIKEWAREEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNNSKR"
BASE COUNT         3583 a         1917 c         1134 g         4141 t
ORIGIN      
        1 taatttaaaa ctactatatt atttaaaaat ttatatttat atatgcaatt ctaaaaactt
       61 aataagaagt gaacttagaa aataaagctt aatttcaaag gcttaaatag actattatta
      121 ttagtctaaa taaactacgt gtatcaaaat tatatattaa attcttctaa aatttttatt
      181 gcaaatcttg cgtttggata tgtaatagac cctccaccaa ttacacctat ttttatagct
      241 tctataattt catctttact tgctccagaa ttaatacaac cctcaatatg ataacaaata
      301 cactcatcac atcggcttaa aattgaaata gctagtccca ttaattcctt atttttttta
      361 cttatagccc cttctgaata agtcttatca tcaaggcttc caaatactct aaaaaactca
      421 tctgtttcac ttaatttttt attgtaaaat cccctatatt ctttcatttc tctagtattc
      481 atcttctacc tcctattttt ttcttataag agtatcacac tgtacttata ttttcaatat
      541 taaatacaaa ttttcatttt atgttcatta gcctaattta tattattttt tgaaatctca
      601 ataagttatc atataagtaa gattttttca gaactaaaat agaaatctca gtaatttctt
      661 tattatctat ctcagttctg aaatatatta ttcttatcca aacattatta tagtgattaa
      721 ttatattagt tatttttacc tgaaatttta tacatcaaat aagaatatac aaatggtagt
      781 attacaatta caaagattac aaagatatta taaaaaggaa tattaataaa tgcattaata
      841 attaataata ttccaccaat aagccaaatc catccagcta aatggtgtgt cttattccaa
      901 ttatcttcat cattaagagt ccatggtaaa attattccta ctgagtaatt tcgtttacat
      961 ttaggaagat agtttccaaa taaaataact attattccta aaattaatcc tgtataaaaa
     1021 ttagactcca aactaactcc tgttgaatat aaaaccatca ttatttgtat aattactgaa
     1081 agtatagcaa ctccccattt tgataaattt tgtataacat ttgaaaaatt agctttctta
     1141 ggctcattct caagtctaaa ccatgtatac aaatggatac atagcattaa caatggtaca
     1201 ccaaaagctg ctataacctt tggagcataa ttatctggat ttccataatt atcaaaatga
     1261 attgcaactt catttggtaa tctattataa aaatatattg atacaataaa tggtataaaa
     1321 catattaaag tagataaaat aaattgttta tttatcgctt ttttcattat ctttttctcc
     1381 cttctccata aattgcgaaa accaaagcat aacctcttcg aaaactgaaa tatttatttc
     1441 gtagaaaata aagttcttat actttgtttc aaatatgagt cctgcctttt ttagttgaga
     1501 tagatgataa gaaattgtag cattcgtcat attaaatttt tcactaattt ctcctgctga
     1561 cattttattc tccctcaaca taaccaatat ttctcttctt atagggtctg aaagagcttt
     1621 gaatgtttct ggaaacccca acaccatcac ctcgctattt agaatttcat ctaaatagaa
     1681 tataacataa catccatttt atttcaataa ctatttagaa ttttttctaa atagttattt
     1741 ctaaatattt attgaaaatt ttcatattaa gattctcaat ttacttaagt cgcttttatt
     1801 taatcttact ttatctaatt tttttaactt aacaaaagtg tattgcacca taattgttaa
     1861 agcaaaaacc taatgactga ggtacttata tatataacaa tacttttaat tttaaattaa
     1921 aagtagctaa acccaattta aaaagtagtt aagtcctatt taaatagaaa agaatgaaac
     1981 gagaaatata ctgtataaaa gtacataaaa acatctacta attagataca actataacag
     2041 aatatattta ttattataat aacaaaagaa taaaaaaaaa ttaatttagc atagccttgt
     2101 taaatataga tttgaatatg aatttaaagt tatgtaaaat taaaaataca ggaatgaaaa
     2161 ttccattcct gtataaaatc taacttttga gtctcagtac aatcttattt tgagacaatc
     2221 ttttttgttt tatattttct tatatttaaa atataacgca tatcttatgc aacatattca
     2281 tatttttctt tatattcttt catcgcatca ttccaagcgt cttgtactgt tacacctctt
     2341 ttattagcaa ttttttgtgc cagttcaatt atcttcatag ctcttcttga tatgttcata
     2401 aacattcctc ctacaaatta ccatatattt catatatata aatcaaggtt atatttttat
     2461 gatatataat ccttctattg taagattaca gtttatatgt gtattatata tgaactatat
     2521 tagaagataa ttagaatttt tataaagcta tatgattaga ttaaatataa aagctttaca
     2581 attacttata aatttaaaaa gtctataaac taaactaatg tttgatatac caattttacg
     2641 accataaata atgccattac taaaaatatt ggttttatca tctttgaacc tttctttata
     2701 gcaactttag cacctacata agctcccatt atcatcacta atgcaaacac tatagcaatt
     2761 ttataatcta cttgaccatt tatcatgaat aataacaatg aagttatatt acttgttaag
     2821 tttaatattt ttgtatttgc agaagcatgt aaaaaatcat atccatatat ttttataaat
     2881 ccaaaagtca aaaaagttcc tgtacctgga ccaaaaaaac catcataaaa acccattata
     2941 agagccataa gcatccctag tcttaaattt ttcttattaa cagcttcaaa attatcttct
     3001 tgacctaaat ttttagaaat aaaggtgtaa atagccacta ttaaaatcat agccacaacc
     3061 aaaacattta atatctcttc acttattgat aatacacatc tcacacctag aacactacca
     3121 ataatagtaa atggtactaa tttttttaaa aggttgttat taatttttcc agactttgca
     3181 tatctatagg cactacttat acatcctgct gatgctgcaa atttatttgt accaatggca
     3241 agatgtactg gcactccaat tgccataaga acaggcatgc ttataagtcc tcctcctcct
     3301 gctatagaat ctacaaaagc agctaaaaac ccccctatac ataaaaaaat aactgaaaac
     3361 ataaaattcc tccatcattc tctttcaatt tttcttacaa tatatattat ataatcttat
     3421 ttaatactta ttatccagca tattctttct atattcattt ggagttatac tattaaactt
     3481 cttaaatatt tgtgaaaaat aactactgtt attaaaacct actttcttac aaatatcact
     3541 aacactcatg cttctatcta acagatattt tttagattct tctatcctaa gaagatttat
     3601 atactcagtt attgaaactt tatttctttc attaaaaata gaactcaaat aatttggact
     3661 tataaaaaaa acatctgata tactattcaa atttatattt tctttataat atttttttat
     3721 atattcgttt atattttcaa ttagatttcc tttatctttt ctccaaaatg ctctattctt
     3781 atcaatacac tctttggaaa attcaaataa tacattccat aaatcttcat cactgtttac
     3841 aagtgatatt tttttattat atttatttct taaataagat ataagtttta aatcctctaa
     3901 atctctaagc actgttctag acaatacaaa tatcatttcc tgaatagtat ctctaacatc
     3961 attatatctt aatttgtttg tatctattag attataaaca ccttctaaat tcatattttt
     4021 aatttctttt tcaatataaa gttctaattc aaagggatat tcttttgaag aattattgaa
     4081 catatctatc ggattaataa tatactcttc ttctaataat ctactatttg cttttttaag
     4141 tgcctcttca agtcttatat tttcttttct actcttcaat atctcctcat cttttatact
     4201 aagttgattt tcaaaaaaat tcctcttagc tatttcctga ataaattcag acatcattaa
     4261 taaattttct tttaatacat ataatctact tttaggattt aatggaaatt cattgtaaat
     4321 tttgtttatg tattttatat caataccaga actacttgat aattcagata aatttttatc
     4381 tatataaata gacttatata ctataaaatt accacattct atatatccaa ctatattttc
     4441 ttctaatact ataggtatct ctaaactgga aacaccatac tcacaatgat acactttttc
     4501 aaagttatca ttatttatgc ttttaacaag ttttttagta cacggtttta tatttttaca
     4561 aatatcgcaa aatttatttt ctttcttagt ctcaaatatt tctttatact ttaagtcata
     4621 tacagatatt ctaagcttca atatattaga tatttgattt gcaagatttt caattatgtt
     4681 tacattaact aactcctgta tattgttttc agaaaatact ataggtattt gtctcaaact
     4741 cgaaaattta tgaggaacat aagtaattaa tattactaaa tcaattttac cagtgtttat
     4801 aatttcatgt tctgaatatg gtgggcaata aactatagat gtttccttta tattgacctt
     4861 tttaccatca attatttgta ttccttctcc ctgaatcaca tataaaagct gttcttcact
     4921 taaatgaaaa tgcttttctt ggcagtttcc cggaaaaaat cttacaattc cagcactaag
     4981 tctattgaat tgtgtttttg atggttcgtg tatccacata acctctcccc aatcaaattt
     5041 ttgtacttca ttatttgact caataaaatc caccataaac tcacctcgta gtttttgtct
     5101 aaatattttt tcttcccttc ttatattaaa ataatatcat catcattaac tttcattact
     5161 ataaaatcat catactcttt taatactcct gtaattttag ttgcacttag tttactacaa
     5221 ttcagttttt taatatattg ataaattcta taattacttt tttattttgt aaaatcagtg
     5281 ttttcccaaa ataattatat ctataaatga aagtattttt actgcttaca tcaatatatg
     5341 ttatcataat tttccttttt attgcatata taaataactg tatataacat tgtccaatac
     5401 tatatagaaa tacatctaag aaagaatatt attaaattgt tccaattaat attatttttc
     5461 ttatagaaat aaaaaactgt tggattcttt tagaaaaatt attttttaca tacctttttt
     5521 gcttaaatag tctatcttct cagctatttt gtatatacac tcttctagtt catgatttta
     5581 ttttaatatc ttgcttatat tgctgaaata attaaattct tttatctatc atattgtctc
     5641 ttatatcaca aacatattgt ttcttatatc acaaaaatga aagcattctt atatcttcaa
     5701 tatataaacc tgcttttttt aatcttgtaa taaatatagg atactctata ttctcttcct
     5761 agaaataact aataccactt atttttctat catatagaaa gtaataaatc tttttcttaa
     5821 taaaattcaa tagtaagtgg aataaaattt tatatgaata agaacattat aaaaatactt
     5881 tatacaatag tcaataaaag atattagctt caaattgtgt tgaagttgta ttatttaaag
     5941 tggaaaagca aataaaaaag aaataatggt ataagtagaa aaatatactt aaaacaaggc
     6001 gaagcctctc ttgtatctaa atcaagtaga gaattaaatc taaaaaagaa aataaatata
     6061 aaagatgact cctgtacatt acaagaatca tcttatggtt tcataatcac tccatggttt
     6121 ttaactgtct gatataaagg tctcagttca ctcatagcat gaccaccttt tccgattgct
     6181 gaccccgtca cttatggaca aaatgtccat aagtgatagt cctctcttaa aaccctctaa
     6241 atagatatat ctcaagcacc tcattgaaac cagttgctgg aaacaaactg aagcgttctg
     6301 gaatagtaaa agaaattgcc accatatcca cgttactgtc ctagttgtta ctttgctaac
     6361 ttcggagcga tttcctctaa caatacgcag tcaaattctg cctttttgct aagaaatggc
     6421 agatggtctg catctggtat aaaaaaaatc tgtttgtctg gcgccactat ttcatcaaaa
     6481 aatttcttcg ctggaagata ggggctatta tagtcatttt ctcccatgat gtagtataca
     6541 gggacctgat agtcaactcc ataatttctt atgtcagctt cttccactaa aaaccgcaat
     6601 acatcacctt gattgtcaat cgcatttgga ttgacaatgg ccgtattttt taactctctc
     6661 aatgaataat atggggttgc cagtatactg attatattgc ccaaatccat atttatagaa
     6721 agcccatact tgctttggta tgttcttact gtcataagag ctgccaaata gcttggacca
     6781 tattttgctg gcggatatgg tgcaatagct tccagcgcct tgatatcttt ttgcttccct
     6841 gcagctttca ccatttcaag tgtcttttca tagcccacgc gctcattgtc caccatatta
     6901 acagtctgcg caatcccaat gtacgcgctg taatcctccg gataggtttg cacagccata
     6961 gttcccagca ctgttcccca gctatgacct ataaggataa ttttctcctt gttgaactct
     7021 tgcttcaaat atgctgtcac ctcatggacg tcttgcagaa cacgctccgc gctcatggtc
     7081 tgcaaaactg ctgccggatc gttcgcaaag tatgttttgc ctacgtttcg ctgatcccag
     7141 ttcaccacag taaaatcatt ttcccactca tactggtaca gatgtaaaaa tggcatcatt
     7201 gaattaccag gacccccatg aacccaaagg atgatggggt tgtctgcgtt ttgtccccgg
     7261 atatataacg cctgctgtat gccgccgatc tccaccattt ccatgcggtc gattccagga
     7321 gcttctattt tatggcttgt acgtataatc gtgggggcta tcagacgcaa accggcaaac
     7381 gccagaacaa aaacagcaac tagtatgcaa agtgtgatta atatcatttt aagtaccttc
     7441 tttcatgaat ttttttagcc atagatatct cctctaacat taaaaaaagt atgttgctat
     7501 tttagttttg ttactctacc tgtcccattg gtttcctttc ataaaaaata ttaattgatt
     7561 ctataatctc atcttccagg atatcaactc ctatttgtct cataagaaac tgtaagaatt
     7621 gaaaccgttt acgaagtgta ggaaagtccc cctgaaatac atctgtatca caccaaaagt
     7681 taaagagcag caaaattacc tctgcgcatt catctgggaa ctctgtgatg atgcttctat
     7741 cctctatgcc ttctcgtaaa acatttgcta atattggtgc tagttttttc aaattatcct
     7801 gtgtaaatgc cagtatgata tgcggacttt ctatcgcgct tgtaatcatg ttactagatt
     7861 cttttatcac ctcatccgtt aaatgccgcc taactaaact tctaagcttc tcttttgctg
     7921 taagtccatg catttcaccg agccattgat ttactgtctc tattatctgt tcaaagcgcc
     7981 tttccatgac cacattaaaa atatcttctt ttgaactgaa gtgatgaaaa attccgccct
     8041 ttgacattcc cgaagcatca acaatatttt gcatacttgt tttgtcgtat cctttttcag
     8101 caaacaactt tgcagaaatg gtaatgatat tttcaatagc ttgctttgga ttgttctttc
     8161 tagtcattat ataactcctt cttattaaaa accgactgtt ggtttttatt atataagcta
     8221 ttcctgcttc tgtcaagtaa cattgtgttt atggcaaaac aataaggttt ggtggttaga
     8281 caggtattaa ttagactaat gcgactttcc cctagatgga acccttggta aatttgcaat
     8341 ctttaccggc tttgattata tctgtaagca aggcaggtat ggccacgcct gccctgtttg
     8401 accctcggat attcattcct atccctcctc tccttagcca ttacaccaaa acgccgctca
     8461 ttgtctccaa tggcggcgtt ttaaagatat gatttctcat gctgctgaaa tatgcaaaaa
     8521 atataagatg ccctttatta tttttcctac aatagaaaag aaaagtattg aagaatgggt
     8581 taatattgga gcaaatatgc tttgttttgc acaagatact acctttatca cttatgttat
     8641 caatcaatta ttatcttttt ataatgagat tgtaccaatt caaaaacaac attaaattat
     8701 aaatctaatt ttatattaaa agagactaat attttatcta tctctattaa ggatattttc
     8761 ttatgtatca taatattagt ctctcttatt attatatact gctatcatca ttagttgtta
     8821 tttatactat ttttataata tacaaattta ttcagctaac gttttgccta attctataca
     8881 ctcatctttt gcttcttcat ttgggtcttc cattgctgta attccttctc caccaataat
     8941 tgtagcacca gcattttgca tacgttcttc ccagtctctc atccattcac aatttcccca
     9001 tccatatgaa ccaaataatc caatctgttt acctgatacc atagattcta attctgaaac
     9061 aaatggctcc atatacccct cttctagttg ctctgctccc atagatgggc aacctaatgc
     9121 aaatttatct tcttctttta aatcacttgg tttcagtaag ctcacatcta aaacttctgg
     9181 tgtcttacct tttaacttca caccttcagc cacaaaattt gccatttttt cagtatttcc
     9241 tgttccactc caataaacaa tatatatttt actcattttt acctcctgta gtatttttta
     9301 attaatcaat agttagttat agctaactaa catctaaaaa acaaaagatt tctttatctg
     9361 aaatctcttt atttttctaa atatagaata tcatagttga tattgaaatt caatacttat
     9421 tgataatatt tatcatttat attttatatt cattttataa tatcaaaaca ctgtatcgta
     9481 tttctaatct ttcatagaaa aatttctaac ttatagtact tttgcatctc ttataaatct
     9541 tttatatgta taagtacata gaaaaacaca ccttttccag atgtgctatc tataatatcg
     9601 tatatttatt tttcaattct aactagctaa tatgtttata aattttatca cttaatacct
     9661 ttatacattg atgtaaaatc ttacacctaa gtaattttta ttatttgtct cgactctata
     9721 taatatagct attttatcat caaatcaaaa attatctttt actattattc ttgtatttat
     9781 agagatttaa atataagtat ccatttcctt gttccgttct aggtattcca agcgctctcg
     9841 tacattttat taattgtgca tacagttcag cttcatctaa atctctttca aactcctctc
     9901 ttgcccattc tttaattagt gctaatgagc ctgatacgta tggtgcactc atactagtac
     9961 cactcataat agcaagttta ttatccatat aagttgatat aatatttctt cctggagcta
    10021 ccaaatctat tgtagtattt gaattactaa acttttcaac caaatagttc tcatttattg
    10081 cacctacttc tattacctca gcataactgg ctggataact atactcactt gtactagaat
    10141 caccatctcc attattacct gctgcacaca ctacagaaat attatttttt actgcttgca
    10201 tgacagcatt ttttaaattc ttatcatctt tgtttccccc aagagacata gatataatat
    10261 caaccttgtt atttacagca aagttaatag cattaattat actttgatat gttccggtac
    10321 catctttatt taatgctttt gctattaata atttacaatc tggagctact cccataactt
    10381 cattattata attagatgca gctataatac ccgccacatg agttccatga ccattaaaat
    10441 cctcatatat atttttattt ccattactgt catcactaaa atttgcacca ccaattattt
    10501 ttccttttaa aagaggatga gatatatcac aacctgtatc tattatacca actacaatat
    10561 ttttaccagt ataaccttca tcccacattc ccctagcatt catttgtttt attccatatg
    10621 ggaatttatt ttttgcacct cttagatttt catttatttc atatggaatt agtttcattc
    10681 ttttattcat catatatcct cctaaaaatt ttattgctta taaaaatcat gaattttatt
    10741 ttataatagg tttataaatc aaattaaaac ctata
//
LOCUS       DAMJVI010000115        10763 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.120, whole genome shotgun sequence.
ACCESSION   DAMJVI010000115 DAMJVI010000000
VERSION     DAMJVI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10763
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.120"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            307..765
                     /locus_tag="PC545_003110"
     CDS             307..765
                     /locus_tag="PC545_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2457853.1"
                     /translation="MEFINFVSDKVIDLKMDCKTKEDFFKEIHNKVFDLGFVKEEFGE
                     KILARENVFPTGLNLGDYGVAIPHTDAEYIKEQFISVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            826..1101
                     /locus_tag="PC545_003111"
     CDS             826..1101
                     /locus_tag="PC545_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDF2457854.1"
                     /translation="MKKILVACGAGIATSTVVCDKVERLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            1160..2434
                     /locus_tag="PC545_003112"
     CDS             1160..2434
                     /locus_tag="PC545_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDF2457855.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKFVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLFLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            2564..3616
                     /locus_tag="PC545_003113"
     CDS             2564..3616
                     /locus_tag="PC545_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2457856.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYVNIKATNVLPIGDMNFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDINPVEK
                     IKELTGGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            3616..4647
                     /locus_tag="PC545_003114"
     CDS             3616..4647
                     /locus_tag="PC545_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDF2457857.1"
                     /translation="MKAVVKTKPGYDNVEVLEVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCPTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMANGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            4739..5551
                     /locus_tag="PC545_003115"
     CDS             4739..5551
                     /locus_tag="PC545_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDF2457858.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVSPVDDKFKSFGWNVIVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            5554..6474
                     /locus_tag="PC545_003116"
     CDS             5554..6474
                     /locus_tag="PC545_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HDF2457859.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILTSIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGINVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            6649..7095
                     /gene="rpiB"
                     /locus_tag="PC545_003117"
     CDS             6649..7095
                     /gene="rpiB"
                     /locus_tag="PC545_003117"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HDF2457860.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEVEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            7125..7790
                     /locus_tag="PC545_003118"
     CDS             7125..7790
                     /locus_tag="PC545_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2457861.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVKADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYISLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIEELKE
                     SIK"
     gene            7900..9402
                     /locus_tag="PC545_003119"
     CDS             7900..9402
                     /locus_tag="PC545_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDF2457862.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVKKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSTVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGAIMITASHLPYYYNGLKFFTESGGLEKTDIKEMLDIAVKNDSVDYEEVNKKGEV
                     IVKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRFKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAQEEKEVRIGINKEDFRTYAEDILKDLDSHVKSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLKGL"
     gene            complement(9714..>10763)
                     /locus_tag="PC545_003120"
     CDS             complement(9714..>10763)
                     /locus_tag="PC545_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2457863.1"
                     /translation="NLLSMYNKKTINKKLAIVFLISIVVQAIIQYLVLIFFMYIYYAE
                     AIAIVILFTLVNTVFHFYIYNFLSVKFNYINNISKNIKNIENGNLKYKLEVIGDDEIS
                     TLAKSINNITGGLETAVDANIKNERMKAELITNVSHDLKTPLTSILSYVDMLKNNDFD
                     RETINDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQSIVEFED
                     KIQNSSLDFIIETPEYAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYIDMFIKEEN
                     IILTFKNISNDKLNLKPEELIERFRRGDISRKTDGSGLGLSIAQNIIELENAHMEIII
                     DADLFKVMLTFRKVN"
BASE COUNT         4295 a         1215 c         1974 g         3279 t
ORIGIN      
        1 tttataaaat agttgttaaa cataaaaaac catttgtaaa aaacaaaaaa atcatttctc
       61 taacaaaaaa aaccacaaaa aatataaaaa agtatggcta gttaagtgat taacaagtaa
      121 aaattaaaat aaaaatcaaa aaaaatttat caatgaaagt cagctaaaat cctggaagag
      181 agcattattt ttgggaaaac attttctttt gtttcacaaa aaaagccact ttttggcaca
      241 caacatgctt tatataaagt caagaaaaac taaggtataa gacaaaggca attaggaggt
      301 gtaaatatgg agtttataaa ttttgtaagt gataaagtta tagaccttaa aatggattgt
      361 aaaacgaaag aagatttctt taaagaaata cataataaag tttttgattt aggctttgta
      421 aaagaagaat ttggagaaaa aatattagca agagaaaatg tatttccaac aggtttaaat
      481 ttaggagatt atggagtagc aataccacat acagatgcag aatatattaa agagcaattt
      541 atatcagtct gtacttttaa tgaaccagta gtgtttagtt ctatggaaga ccaagatgaa
      601 aaagtacctg taaatttggc tttcgtatta ggacttaatc aaccacacag tcaattatct
      661 gtattaacag aattaatggg aattatgcaa aataaagagt tagttgataa gttgatgagt
      721 tctactgata aagaagaagt tttaaagaca ctaaagtctt tataaaaata aaaaagtact
      781 aaaaataaaa aatataaaaa taaaaattat taggaggaaa taaaaatgaa aaaaatatta
      841 gtagcatgtg gagcaggaat agcaacatca acagtagttt gtgacaaggt agagagatta
      901 gttaaggaaa ataatataca agcagaagtt atacaatgca aaatagctga atgtgcaaca
      961 aagcaagaag gagcagattt aatagtatca acaactatac taccaacaac ttataatata
     1021 ccagcaataa aagcaacagc atatataact ggagtaaata cagcagcatt agataaaaaa
     1081 atattagatg ctttaaaata atcctatcat agatacattg aaataatatt ttataaaaca
     1141 gaaaaaggag gatttataaa tggataaatt attagcagta gtacaataca ttttaaatat
     1201 gggaccgaca gtaatactac caataacaat tgtagtacta ggaatgatat ttagaattaa
     1261 gtttacaaaa gctttaaagt caggtcttac aattggtata ggatttgtgg gtataaactt
     1321 agttgtaaac ctattaacat caacactagg gccagcggct caaggaatgg ttgaaagatt
     1381 cggacttaat ttaacagtaa tagatgttgg ttggccaaca acagcatcag caacatgggc
     1441 ttcaccagtt gcaccaatat taataccagt ttgtttatta gtaaacttac tattattata
     1501 cttcaataaa acaaagatat taaacgttga tatatggaat tattggcact ttatagtagc
     1561 aggtgcagtt ggatatatag taacaggaag ttatgtttgg gctatattct gtgcaatact
     1621 aatggagtta ttagtactgt ttattgcaga aaagacagca aaaaatgttc aggagtttta
     1681 tgatttagaa ggtatagcat taccaactgg ttcaacagct tcatttgctc cacttggata
     1741 tttagtaggt aaatttgttg aaaaaatacc aggaataaat aaaatacaag cagacccaga
     1801 ctctatacaa aaaagatttg gtatatttgg agaaccaatg atgatgggga ttatactagg
     1861 tataatactt ggagcacttg cgggatatga tgtagcagga atattcaatg taggtatatc
     1921 aatgggtgca gtaatgttac ttatgcctag aatggttaga atattaatgg aaggtttaat
     1981 tccaatttca gaatcaataa gagatttctt acaagcaaaa tatggagaca gagaacttta
     2041 tataggtctg gatgcagcag tagcaactgg acatccagca gttatatcaa cagctcttat
     2101 acttgttcct ataactttgt tcttagcagt tatactacca ggaaacaaag ttttaccatt
     2161 tggagactta gcgacaatac cattttatat ggcatttata gtgtgtttta gaaaaggtaa
     2221 tatagttcaa tcagttataa caggaacaat agtaatagct atatcacttt atatggctac
     2281 aaactttgca gatgttcata cacaattatt acaacaagca aatatgatgc cagaaggaac
     2341 aacaatggtt acaagtatag atacaggtgg aaacttgcta aaatggggaa tattgaaatt
     2401 atctcaatta gtttcttcat ttggaatatt ctagtatgta gttaatagag attactcttt
     2461 tttagtaaag tatgcgaaaa tttaacattt agcagctact aaaaaagagt aatatttttt
     2521 ataaattttt aaaaatacaa aatacaagag gaggagattt atcgtgaaag cagcagtatt
     2581 acatggcaca aatgatatga gatttgaaga tatagaaata aaaccatgtg aaagtgatga
     2641 agtaaaaata aaagttatgg cagcaggaat atgtgggtca gatcctccaa gagtattaaa
     2701 acattggaaa tatccagttc cagctatacc aggacatgag ttttctggag taattgctga
     2761 agttggtaag gatgtaaaaa atgtaaaagt tggagataga gtagttgcaa ttccttttat
     2821 accttgtaat gaatgtgaat attgtaaaag aggattattt tcattgtgtg atgaccatgg
     2881 aatgttagga gctaaaagtt ttggagcttt tgcagagtat gtaaatataa aagctacaaa
     2941 tgtacttcca ataggagata tgaactttga agatgctgca atgatagagc cactagcagt
     3001 tgctatgcat ggagtactta atataggtgt tcaagttgga gacactgtag ctgtaatggg
     3061 tagtggaaca atggggcagt tggtaataca aggacttaaa atagcagggg caggaacaat
     3121 tatagcagtt gatatatctg ataataaatt aagagaatca aaggaattag gggctgatat
     3181 aataattaat gctaaagata ttaacccagt agaaaaaata aaagaactta ctggtggaaa
     3241 aggtgttgac atagctcttg aatgtgcagg ttcaaaaata actcaagaac aatgtttact
     3301 tataactaaa aagaaaagta agataggatt tttagggata gcatattcag atataacttt
     3361 atcagaagaa gcatttgaaa atatatttag aaaagaacta gaattaaaag gtttttggaa
     3421 ctcttattct gcaccatttc caggacaaga atggactaag ggaatcaatc tagttaatga
     3481 aggaaagata aaattaaaag aaatggtatc tcatagattc tcacttgaag atacttacaa
     3541 agcatttgaa atgataagag atagaaaaga agaatttaac aaaatattaa ttttaccaca
     3601 aggggttgaa aaataatgaa agcagtagtt aaaactaaac caggctatga taatgtagaa
     3661 gttttagaag tagaagaacc aaaagctaca ggagacaagg ttaaaataaa agtagaatat
     3721 agtggtatat gtggttcaga tatacattca tttaaaggtg aatatgcaaa tataaaagcc
     3781 ccagtaacat taggtcatga attttctgga attgttgtag aagtaggaga agatgttaaa
     3841 aatataaaag taggggatag agttacatca gagactactt ttgcaacttg tgagaaatgt
     3901 atatattgtg aaactaaaga ttataactta tgcccaacta gaaaaggtat tggtactcaa
     3961 gtaaatggaa gttttgctga gtatgtatta tcaagagaag aaagcataca tgtacttcct
     4021 gaaaatgtat ctttattatc agcagccctt acagagccac tagcttgttg tgttcatgca
     4081 gctcttgaaa agactactat agaaagttct gatacagttt taataatagg accaggacca
     4141 attggtttat tattagctca agttgttaaa tctcaaggtg ctacagttat aatgtctggt
     4201 gttacaaaag ataaggaaag attagaaata gctaaaaatc ttggagtaga tagaactgta
     4261 aatgtaatgg aagaaagttt ggcagatgta gtgaatgaaa tgactgatgg aaatggagta
     4321 aacaaaggat ttgattgttc tgggtttatg ccagcagtta acgaagcctt aagactgctt
     4381 agaaaaaaag gtactttcgt acaagttgga atctttgcta aaaaattaaa tgaaatggac
     4441 caagaagcaa taatccaaag agagttacaa tatataggaa gcaggtctca aaagccaagt
     4501 tcttggatta cagcattaga tttaatggca aatgggaaag taaatacaga agctttagtt
     4561 actagaactg ttggtttaga cgaatttaaa gaaggtatta cagctttaat gaacggggaa
     4621 gaaataaaag tagcagtaaa atcttaaaaa aacatttgta ttattttaaa aaaaattgta
     4681 aaatctactg taggataaaa aaatgtaagg tcttaaaaaa taacaggagg aagaaagtat
     4741 gagcactctt agagatcatt ctaataatat tagaaaaaac ataataaaaa tggttgcaga
     4801 agctaaaagc ggacatccag gtggttcatt atcaatagct gatatgttga cagtattata
     4861 ttttgataag atgaacgtat gtgcagaaaa tccaaaaatg gctgatagag atagatttgt
     4921 tctatcaaaa ggtcatgcag caccagcttt atatgctaca ttagcagaaa aaggattttt
     4981 cccagaagaa gaattaataa cactaagaaa atttggttct aagttacaag gtcacccaga
     5041 catgaaaaaa gtagcaggaa tcgatatgtc tactggttct cttggtcaag ggctatctgc
     5101 agcaaatggt atggcacttg caggaaaatt agataacaaa gactatacag tatatactat
     5161 attaggtgat ggagaaatcc aagaaggtca aatatgggaa gctgcaatga cagcatcaca
     5221 ttacaaatta gataatttaa ttgcatttgt tgacttaaat ggattacaaa tagatggttc
     5281 taatgaagaa gtaatgaatg taagtccagt agatgacaaa ttcaaatctt ttggatggaa
     5341 tgtaatagta ataaatggac attcttttga agaaatagga aatgcagttg atgaagctaa
     5401 aaaagttaca ggaaaaccaa ctgtaataat agctaaaaca gtaaaaggta aaggtgtttc
     5461 atttatggaa aataatgctg catggcatgg aacagctcca aatgcagaac aagcagagca
     5521 agcattgaaa gatttagaag gaggtctata ggaatgggaa tagcaactag agaagcttac
     5581 ggacaagtgt taaaagaact tgcagaaaat aaagatatag tagtattaga tgcagattta
     5641 ggaaaagcta caaaaagtat atcatttaaa gaagttgccc cagatagatt ttttgacatg
     5701 gggatagcag aaggtgacat gattggtaca gcagcaggtc ttgctacttg tggaaaaata
     5761 ccttttgcga gtacttttgc aatatttgca gcaggaagag gctatgagca aataagaaac
     5821 tctgtagcat atccaaattt aaatgtaaaa atagcagcta ctcatgcagg tgtaacagta
     5881 ggtgaagatg gtggtagcca tcaagctata gaggatatat ctctaatgag aggtatacca
     5941 aatatggttg tattaaatcc agcagatgct ttagaagcaa gacaagctat tcttacatct
     6001 attgattata atggacctgt ttatataaga ttaggtagag ctgcaactcc tgatgtaaac
     6061 agtgaaaatt ataaatttga aataggaaaa ggaactgttt taagagaagg ttctgacata
     6121 acagtaatag ctacaggaat aatggtagca aaagccttag aagcagcaga agaacttgct
     6181 aaagaaggta taaatgtaga agttgttaat atatctacaa taaaaccatt agatgaagca
     6241 ttaataaaag aaactgctaa gaaaactgga aaagtagtta cagcagagga gcacagtata
     6301 ataggtggtt taggttcagc tgtatgtgaa gctttagcag aaactaaaga tgtagtagtt
     6361 agaagaattg gtgttaaaga tgtatttggt caatcaggta caccagctga tttattaaaa
     6421 cactatggtt taactacaga agatatagta aaaaatataa aagagttgtt atagatataa
     6481 atatatctaa ataacaaatt gatttgacat aagtcaatta aagctgtctt aaaaatagaa
     6541 agaaatttct acaatgggac agctttattt ttacaatact ttatttttat gataattata
     6601 aaatagtctg aaagtaatca aaaaaattat aaaaagaggt gctatttaat gaaaatatct
     6661 attggatgtg accatggtgg ttatgaatta aaggaatata taaaggaaaa tttaataaat
     6721 aaaggtatag aagttgaaga tgtgggtaca aattcaaaag aaagtgtaga ttttccaata
     6781 tatgcaaaaa aggtaagtca aaatgttcaa agtaaaaaat ctgatttagg catactttgt
     6841 tgtggaacag gaataggtat gtctattgca gcaaataagt ttaaaggaat cagagcagct
     6901 gttgtttctg actgtttttc agctaaggca actagagaac acaataatac aaatgtactt
     6961 tgtttaggag aaagagttat tgggaaagga ttagcaatga gaattgttga agaatggtta
     7021 aattcaagtt ataatggaga aagacatgca agaagattac aaatgattga tgatatagaa
     7081 aaagaaaatt tataaatata taatttacaa caagggggag cgttatgtct attatttgtg
     7141 cttcaataat gtgtgcagac caaatgaagt taaaagagga attagaagct cttgaagaag
     7201 caggtgtaaa attacttcat tgtgatgtta tggatggaat atttgttaaa aacttggcta
     7261 tgggaccaga gttattaaaa tcaataagtg aaaatacaac aataccttta gacatacact
     7321 tagccacaga aactccagat aaatatattg atatgatgtc ttatataaag ccaaaatata
     7381 tttcttttca tgtagaatca agtacaaatg taaaagctga tatacaaaag ttaagaaatt
     7441 atggtatagg gccagtgtta gcaataagcc ctcaaacaag tgtggataaa atagaagaat
     7501 atataagttt agttgagggt atcttagtaa tgacagtaaa tccagggttt gcaggtcaaa
     7561 agtttaatct ttctgtattg gataagttgg ataaacttac agagatacta aaagattatg
     7621 ataatcctcc atttatagag gttgatggaa atataaataa ggatactatt aaacttatga
     7681 atggtaaaaa agtagatata tatgttgttg gtacttcagc tttatttaat gataagcctc
     7741 caatttcata taaagataaa atagaagaac taaaagaaag tataaagtaa ccatgtaata
     7801 aaattataat atgtttctgt ttaatagtaa aaatttaggt ggaaacaaga aattttataa
     7861 tatttaataa aaattttggt tgagtaagga gaatttttta tgggagacct atataaacta
     7921 caaaatggta ctgatattag aggaatagcg tatgaaaatg attctaaaga agttaaccta
     7981 actgtagaag aagtaaaaaa aatagctaaa gcgtttcata tttggttaaa agaaaagact
     8041 aagaaagata aagtaactgt tgccatagga actgattcta ggataacagg aagccaattt
     8101 agaagtactg ttattgaaac tttaactaat gatgattgta atgtaattga ttgtgagatt
     8161 gccacaactc cagctatgtt tatgacaact attatggatg gatacaattc tgatggggcg
     8221 attatgatta cagcaagtca cttaccttat tactataatg gtttaaagtt tttcacagaa
     8281 agtggaggtt tagaaaaaac tgatataaaa gagatgttgg atatagctgt taaaaatgat
     8341 agtgtagact atgaggaagt aaataaaaaa ggtgaagtta tagtaaaaaa tttaatagaa
     8401 gattattcta atttattgat agataaaatt agaaaaggtg taaattcaag taaaaattat
     8461 gaaaagcctt tttctgggct taagatattg gttgatgcag gaaatggggc tggtggattc
     8521 tttgctgaaa aagttttaca catactggga gcagatacga caggtagtca gtttttgaac
     8581 ccagatggta tgttcccaaa tcatatacct aatcccgaaa acaaagaagc aatggaaagc
     8641 atatgtaaag ctgtgttgga taataagagc gacctaggaa taatctttga tacagatgta
     8701 gacagagctg ctattgtagg taaaaatggc aaaccaataa ataaaaatgc tcttatagct
     8761 gttatatcat caatagtatt agaagaacat ccaaaaacag ctatagtaac agattcgatt
     8821 acttctgaag gacttgctaa atttataaat gaattacaag gaagacatca tagatttaaa
     8881 agaggatata aaaatgtaat aaatgaagct ataagattaa atagtgaagg tgaagagtgc
     8941 catttagcta tagaaacgtc tggacatgct gctttaaaag aaaattattt cttagatgat
     9001 ggggcatatt taatagctaa aattttaata aaagtagcaa agctatcctt ggaaggaaag
     9061 actatagaag aattgataga aaaccttgaa gaagctcaag aagagaaaga agttagaata
     9121 ggcattaata aagaagattt tagaacatat gcagaggata tattaaaaga tttagattct
     9181 catgttaaga gtattgatgg atggtctgta gcaccaaata attttgaagg cataagagtt
     9241 aattgtgatg aatcaaatgg agatggatgg tttttactta gaatatcact acatgaacca
     9301 ttattagctt taaatattga atcaaataaa attggcggag cagaacaaat ttattctaag
     9361 ttaaaacttt tcttagaaaa atatgattta aaaggtcttt agggtaaata gaaagaatat
     9421 atttatattg aagaataaat gtaaatttgt acctaagatt acaaatatta caaaattgta
     9481 atcttaggta acagagatgt aactgattat ggaaacacta tgtgatatta tagatacaga
     9541 gagatgaaat atgtgatgaa ttatagagaa atataattta aaatgtatta atatcacacc
     9601 aaaaaggtat aatcaaaaaa tatgtaaata taaaccccaa catttagaaa aagacctcta
     9661 aggaggtttt tttcttgttt aaataactta aaatatattt ttatattaat atatcaatta
     9721 acttttctaa aagtaagcat taccttaaat aaatcagcat ctataattat ttccatgtgt
     9781 gcattttcta attcaattat attttgagca attgataaac caagaccaga gccatctgtt
     9841 tttctagata tatcacccct tctaaatctt tctattaact cttctggttt tagatttagt
     9901 ttatcatttg aaatattttt aaacgttaat attatatttt cttcctttat aaacatatct
     9961 atatagaccc tagtgttagg catagagtat tttagtatat tgcaaattag attctcaaaa
    10021 actctccaag tttttttacc atctatcatg gtaaaaacag catattcagg tgtttctatt
    10081 ataaaatcta aactgctatt ttgaattttg tcttcaaatt ctacaatact ttgaattaaa
    10141 agttctctta tatcggtttt tgttatatta aattctacat ctccactact tagcttagat
    10201 gcttcaaata tgtcatctat taataatttt aatctttgtg attttttatc taaaatattt
    10261 atatagtcat ttatagtctc tctgtcaaag tcattatttt ttaacatatc aacatagctt
    10321 aatatagatg taagtggagt ttttaagtca tgagatacgt ttgtgataag ctctgccttc
    10381 atcctttcat tttttatatt agcatctaca gcagtttcaa gaccgcctgt tatattattt
    10441 atacttttgg ctaaagtaga tatttcatca tctcctataa cttctaattt atactttaaa
    10501 ttgccatttt ctatattttt tatatttttt gatatgttat ttatgtaatt aaacttaaca
    10561 gataaaaagt tgtatatata aaaatgaaaa actgtgttta caagtgtaaa taaaattact
    10621 atagctattg cctcagcata atatatatac ataaagaata tcaaaacaag atattggata
    10681 atagcttgta caactataga tattaaaaat actatagcta attttttatt tatagttttt
    10741 ttattataca tactaagtaa gtt
//
LOCUS       DAMJVI010000116        10626 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.121, whole genome shotgun sequence.
ACCESSION   DAMJVI010000116 DAMJVI010000000
VERSION     DAMJVI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10626
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.121"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            232..3129
                     /locus_tag="PC545_003121"
     CDS             232..3129
                     /locus_tag="PC545_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2457864.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIRVGYCD
                     DYGIISSSKEHSYTGYGYDYLREISKYTRWEYEFVKGSWEECLDRLEKGEIDLLGPLQ
                     KNDERNKLFNFPKLNSGYEYSVLYTKDSNNNMFYEDISSFKGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIINAKGMKIVSKFSVEPFYFA
                     TAKYKPNLVKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEMNFIKANPK
                     LTMVYDSEWSPVEYYDKESNSFKGISSNLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKANMLCGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYKSIESCLNAINEGKANLMILSSYQFDELLREGKSENLSVISVL
                     DTSYGMRIGVSNKTDPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQIISFVCILFLIAIKYIIYNKKKKEDYLKKIAYTDPLTGADSIDKFKINSNKLFAK
                     NNPEEYALFYIDVDKFKYINDMFGYDMGNDTLIHISNTIASELKEDEIFARVSADHFV
                     LLIKYKTDDDIKTRLNNIYNKVQILSNPKINYYKLILDCGIYKISKSDNDINTIMDRA
                     NTARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFIVYFQPKYSLSDY
                     QIIGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQK
                     VVPISVNLSRMHFVNSNFIEKFKLIVDKYKIPTRLIELELTETAVLDNIEGLLDTMNN
                     LKEKGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMA
                     KELKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            3450..3662
                     /locus_tag="PC545_003122"
     CDS             3450..3662
                     /locus_tag="PC545_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2457865.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            3675..3896
                     /locus_tag="PC545_003123"
     CDS             3675..3896
                     /locus_tag="PC545_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDF2457866.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEVTVRDYELSIRKSDAEKIIVE"
     gene            3996..6152
                     /gene="feoB"
                     /locus_tag="PC545_003124"
     CDS             3996..6152
                     /gene="feoB"
                     /locus_tag="PC545_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDF2457867.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKDAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISSIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKIPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSAKGVLIHM
                     WDRGKAFIIKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVATVTGLVAKENVVGTFGVLFGISDATEQDPTLLASVASMFTVASAFAFMAF
                     NMLCAPCFAAIGAIKREMGSWKWTWITLGFQTLTAYIIALLINQVGSLVLGTGGSIAG
                     AIISIFIAVAVVFVVLTYSNKNMKKEKMGKLSYMKN"
     gene            6178..6330
                     /locus_tag="PC545_003125"
     CDS             6178..6330
                     /locus_tag="PC545_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDF2457868.1"
                     /translation="MATFIIAAIVVVLMALAVMYMVKNSKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            6510..7220
                     /locus_tag="PC545_003126"
     CDS             6510..7220
                     /locus_tag="PC545_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2457869.1"
                     /translation="MFKFDVTNELMCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPS
                     NGGGLGRALLGHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESE
                     NLLAFSEQLNYSVKLIGSGVISQKGLFTTHLVNKTGQNQDVAIITDGNPLILEGPCCV
                     DPDALVAWTGREPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKT
                     DSTPSDRMSSGGLRLGID"
     gene            7305..8075
                     /locus_tag="PC545_003127"
     CDS             7305..8075
                     /locus_tag="PC545_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457870.1"
                     /translation="MFEKIHKRLKGYYIFLIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVLFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SKKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            8068..8799
                     /locus_tag="PC545_003128"
     CDS             8068..8799
                     /locus_tag="PC545_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457871.1"
                     /translation="MVRSGFLLENIYKKYLVDNKEHLVLDNISLNISSEEITVILGES
                     GCGKTTLLRILAGLENATSGNIYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVNIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            8833..9792
                     /locus_tag="PC545_003129"
     CDS             8833..9792
                     /locus_tag="PC545_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2457872.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNDNIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDEVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(9890..10486)
                     /locus_tag="PC545_003130"
     CDS             complement(9890..10486)
                     /locus_tag="PC545_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HDF2457873.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DVNREEKINDIYKYLYKCKKIDFIVIDKEKKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKSDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
BASE COUNT         4109 a         1098 c         1896 g         3523 t
ORIGIN      
        1 ttttgaaata aaattaatca ctagaattat agtgttagtt gtgcttttat gacttataag
       61 aaactaatat tagatagtgt atttaaatag ataataataa attcattata gttgaataag
      121 aatattatat gagaatattg caatttgata tatttaccat tacaataaaa gtagttacaa
      181 ctagaaaacc aaataagtta aagtaagttg gcaaattgga ggtttttaaa tatgaaagca
      241 tttaaaaagc acttcttgat actttctttg atatttttac ttattatatc atgttttaac
      301 aatatactat gctttgctga tgagtctgaa aattcaaaaa aaataagagt tggatattgt
      361 gatgactacg gaataatatc ctcatcaaaa gagcattctt atacaggata tggttatgat
      421 tatctaagag aaatctcaaa atatacaaga tgggaatatg aatttgtaaa agggtcttgg
      481 gaagaatgtt tagacagact agaaaaaggt gaaatagact tacttggacc tttacagaag
      541 aatgatgaaa gaaataaact atttaatttt cctaaactaa attcaggata tgaatatagt
      601 gtactatata caaaagatag taacaataat atgttttatg aagatataag ttcctttaag
      661 ggaatgagag tagctgtact tagaggaaac tttcataata ttgcatttga gaagtataga
      721 gaagaaaata atttttcagt tgaatatatc tactgtaatt ctattgatga attaattgag
      781 tctgtcaatg aaaaaaaggc agatgcattt gtctgtggaa gtattataaa tgctaaagga
      841 atgaaaatag ttagcaaatt ttcagtagag cctttttatt ttgctactgc aaaatataaa
      901 cctaatttgg taaaagaatt agattatgct ttaaaagaat taaaaataaa tgatatgtat
      961 tatgagttgg aattatataa aaaatatttc aaaagagagg tcaataattc catagcattt
     1021 actcggcaag aaatgaattt tattaaagca aacccaaaac ttactatggt atatgattca
     1081 gagtggtctc ctgtagagta ttatgataag gaaagcaatt cgtttaaagg tataagttcc
     1141 aatttaatgt caattatatc aaaaaaatgt ggtataaaat ttgaatatat taaaacaaaa
     1201 aattataatg agagtcttga ttacataaaa agtggtaagg caaatatgct atgtggctca
     1261 atcaatgaaa atgataaggc aaaacgattt aatatgaaat tgacaaatcc atacataaat
     1321 attccaatga ttatggtagg aaaactggat accaatctca ataatgattt aaatattgct
     1381 ttaacgagta gttataagtc tatcgattct tatatagaaa aaagttttga aaatgctaag
     1441 acaacatctt ataagagtat agaaagttgt ctaaacgcta taaatgaagg taaagcaaac
     1501 ttaatgatac ttagttctta tcagtttgat gaacttttaa gagaaggaaa gagtgagaat
     1561 ttatctgtta tatctgtttt agatacaagc tatgggatgc gtataggggt atcaaataaa
     1621 actgacccta tattagtatc tattttaaat aaatcaatag acaaaattac tgaagaagaa
     1681 ttaagtgatt gtatatattc taatactatt gacaaacctt ataaagtacc atttggtgtg
     1741 atttttaaag aatattcaat acaaattatt tcttttgtat gtattttatt cttaattgct
     1801 attaaatata taatttataa taaaaagaag aaagaggatt atctaaagaa gatagcatat
     1861 acagacccat tgacaggagc agatagtata gataaattta aaataaattc gaataaactg
     1921 tttgctaaaa ataatccaga agaatatgca ttgttttata tagatgttga caaatttaaa
     1981 tatataaatg atatgtttgg atatgatatg ggaaatgata ctttgataca tatatcaaat
     2041 actattgcaa gtgaattaaa ggaagatgaa atttttgcaa gagtttctgc tgaccatttt
     2101 gttcttctta ttaagtataa gacagatgat gatattaaaa caaggttaaa caatatttac
     2161 aataaggttc aaatacttag taatcctaaa ataaactatt ataaattgat tttagattgt
     2221 ggtatatata aaataagtaa aagtgataat gacataaata ctattatgga tagagctaat
     2281 actgcaagaa aaactataaa aggtggtcat aaaaattcat ttgcttttta cgataaggaa
     2341 atgcataaaa aaatattaaa agagaaagaa attgaaaatt caatggtaga tgctcttaat
     2401 aatggagaat ttattgttta ttttcaacct aaatatagtc tttcagatta tcaaattata
     2461 ggggcagagg ctcttgttcg atgggataat ccacaaaaag gtctaatacc accaatagaa
     2521 tttattcctg tatttgagag gaatggtttt attgtaaata ttgatttcta tgtatttgag
     2581 gaagtgtgta aaaagattag agaatggatg gatgaaggac aaaaagtagt acctatatct
     2641 gtaaacttgt ctcgaatgca ttttgtaaat agtaatttta tagagaagtt taaattaatt
     2701 gtagataaat acaaaattcc gacaagactt atagaattag agctaacaga aacagcagta
     2761 ttagataata tagaagggtt acttgataca atgaacaatt tgaaagaaaa gggatttgta
     2821 atctctatgg atgattttgg tacgggatat tcctctctta atcttttaaa agaattgcct
     2881 gttgacatat taaaactaga tagagcattt tttacagaaa aagatgagag taataatgaa
     2941 aaaattgtta tatctaatgt cattaaaatg gcaaaggaat tgaaaatgaa agtaatatca
     3001 gaaggtgtag agactatttc gcaagtagaa tttctaaagc aaattgggtg tgacatggta
     3061 caaggatatt tgttttctaa gccaatgcct gtaaaagagt ttgaaaaaat tgcttttaaa
     3121 aaagaataaa gcaatgtgta agtaatgata tatataataa aggtgcttta atattagttg
     3181 agtatactat tttagagcat ctttattttt gtattaattt ttacgatttt attaataaaa
     3241 taaatcaaat gagaaaaaaa atcaaaagaa tacaataatc acttgcaaaa aatatactat
     3301 tgtgatagac ttaaatcaaa attaggaatt gtaaaaagaa ggaaaaaata ggatattatt
     3361 ttaatagatt aagatttaag gtattgtaat ttatatataa gataacgatt gcttgtagat
     3421 ttaaattagt ttaagaggag ggatattata tgatgccatt gatactatca aatacaggtg
     3481 aagaagtagt tataaagaaa atttcaggaa gtgataagac gaggactttt ttaaatagta
     3541 taggttttgt agtaggtaca aatgttaaaa tagtttctaa gatagatggt aacttaataa
     3601 tagacgtaaa agatactaga gttgcattag ataaaaaaat ggcaagtaga ataataatat
     3661 agggaggtaa tgacatgaac agactgaagg atattaagtg tggagagact gtaaaagtca
     3721 aaaagttaga aggagaagga gctacacgaa gacgtataat ggatatgggg attacaagag
     3781 gcgttaatat atttataaga aaagtagctc ctcttggtga cccaattgag gtaacagtca
     3841 gagattatga actatcaatt cgtaagtcag atgcagaaaa aattattgtg gaataataaa
     3901 gattaattag agtagagtta atgtaattat ataatgagaa tgattttcta tatcatgaat
     3961 agtgatgtat ttaaataata atggagggaa tggaaatgtc aataaaaata ggtctcatag
     4021 gaaatcctaa ctgtggtaag acaacaatgt tcaatgggct tacaggctca tcacaatatg
     4081 ttggtaactg gccaggagta acagttgaaa aaaaaggtgg aaaattaaaa ggaaataaag
     4141 atgtggaaat agtagattta ccaggtattt attctttatc tccatataca ttagaagaag
     4201 tagttacacg taattttatg ctagatgata agccggatgc agtaataaat attgtagatg
     4261 catcaaatat agagagaaat ttatatctta caactcaagt tttagagcta ggtataccga
     4321 ccgttatagc tcttaatatg atggatattg taaataagaa tggtgataag ataaatatta
     4381 aagaactatc tgaagttata ggttgcccag tggtagaagt aacagcagtt aaaggtcaag
     4441 gaattatgga ggctgctgaa aaggcagttg aacttgcaag tagtaacaat aaattaaatt
     4501 tcaaattacc ctttgttgat gaaagtaagg atgcaataga aaaaattgaa aaaataatag
     4561 aagaaaagac tccatatata gatgtagaaa cacgctggtt agctataaag ctatttgaga
     4621 gagatgaaaa tgttatacaa aaacttgata tttctaaaac aatattgaat agtattgaag
     4681 aaataactag aaattgtgaa gatgaattgg atgatgatag tgaaagtata ataacagcca
     4741 atcgttatga atttattagt tcaataattt caagtataat aaagaagaat cgaaaaggta
     4801 aggaaactgt atcagataag atagataaga ttgtaacaaa tcgtatacta gcattaccta
     4861 tttttgcact tataatgtgg ggagtttact atatagcagt aagttcattg ggtactatag
     4921 caactgattg gacaaatgat gtactgtttg gagaaattat tcaaggcaat gtaagtaatt
     4981 ttctagcttc tttaaatgta gcagagtggt tacaaggact tgtagttgat ggtctaattg
     5041 gtggagttgg agcagtacta ggatttgtac cacaaatcat gcttttattc ttattattat
     5101 caatattaga agattgtggg tacatgtcac gtgtagcttt tataatggat agaattttcc
     5161 gtaagtttgg tctttcagga aaatcattta taccaatgct tataagttca ggttgtggag
     5221 ttccaggagt tatgtcaact cgtactatag agaatgatag agatagaaaa atgactatta
     5281 tgttaactac attcattcca tgtggagcta agattccaat aatagcatta tttgcagggg
     5341 cattatttgg aggtgcttca tgggtagctc catcaatgta tttcttaggt attgcaatga
     5401 taatcatttg tggaattata ttaaagaaaa ctagtttatt tgcaggagaa ccatctccat
     5461 ttgttatgga attacctcaa tatcatattc caagtgcaaa aggtgtttta atacatatgt
     5521 gggatagagg aaaagcattt ataatcaaag caggaacaat tatatttgtt gcttgtggag
     5581 taatctggtt cttacaatca tttaattggt cattacaaat ggtagatgct ggagatagta
     5641 tattagctag cttaggaaat atagttgcac caatatttgc tcctcttgga tttggaaatt
     5701 ggcaatcatc agtagcaaca gttacaggtc tagtagctaa ggaaaatgta gttggaacat
     5761 ttggagtttt atttggtata tcagatgcaa cagagcaaga cccaacactt cttgcttcag
     5821 ttgcaagcat gtttacagta gctagtgcgt ttgcatttat ggcatttaat atgctttgtg
     5881 caccttgttt tgcagctatt ggagccataa aaagagaaat gggttcatgg aaatggacat
     5941 ggattacatt aggattccaa actttaacag cgtatataat tgctcttctt ataaatcaag
     6001 ttggaagttt agttttaggt actggaggaa gtattgctgg ggcaataata tctatattta
     6061 tagcagtagc agtagtcttt gtagttttaa cttattcaaa taaaaatatg aaaaaagaaa
     6121 aaatgggtaa attaagttat atgaaaaatt aattcatata aataaggagt gatgaatatg
     6181 gctacattta ttatagcagc tattgtagta gtattaatgg cactagctgt aatgtatatg
     6241 gttaaaaatt caaaaaagaa tggtagcagt tgtggatgtg gttgttctgg atgctcaagt
     6301 agtaagagtt gtcatagcgc taagagataa ataggataag agcctgataa aaagttaaat
     6361 tgctttatta tcaggttctt attttttgta taaataacaa atttcatatt tgaagtaggt
     6421 tagactatgc tgtaatttta ctgtataatt atatataaat atatgattag gagggattat
     6481 tgctaaatta agcaatataa gtgaatatta tgtttaaatt tgatgttaca aacgagctta
     6541 tgtgtatagc aaggggaagt ggtaaatttt ttgcaaaaaa aggtgctatg gtagccttta
     6601 aaggaaattt taattttgaa aaattgctgt taggtccaag taatggcggt ggattgggaa
     6661 gagcattatt aggtcatgtt cagaggtcat tgacaggaga acaaatgcca ataatggtgg
     6721 ttgaaggtga aggtgaaatt tacctagctc aaaatgctta ccatgtagat gtaatatcaa
     6781 ttgacccagg tgattccatt tatgttgaga gtgaaaactt attggcattt tctgaacaat
     6841 taaattacag tgtaaaattg attggttcag gggtaatatc tcaaaaaggt ctttttacta
     6901 cacatttagt aaataaaaca ggtcaaaatc aagatgttgc tataataaca gatggtaatc
     6961 ctctaattct tgaaggacct tgttgtgttg acccagatgc attagtagct tggactggaa
     7021 gagaaccata tcctaaagta tcaaaactat catggaagac ttttattggt cagacttcag
     7081 gagaatctta tcatatggaa tttgttgaac ctggacaaat agtcattata caaccatctg
     7141 aaagattgtc tggcttaaaa acagattcta caccttctga tagaatgagt tcaggtggat
     7201 taagacttgg catagattaa atcttatgat atagcttaat tattatttaa gatattaaaa
     7261 aataaaaagg gtagcaggga tagatatgtt aaaaggagtt ttatatgttt gagaaaattc
     7321 ataaaaggct taaaggatat tatatttttt taataatttt aattttatgg aagatagtat
     7381 gtgttttggg tatatggagt agctatattc tgcctcctcc tgaaatagtc ttagatactt
     7441 ttataaaaat gatatatgac ggttctattt tcttaaatgt ttatgccagt gtaagaagga
     7501 tattaatagg ttttttaata agttcattga tggctatacc acttggagtt ttatttggag
     7561 taaataaaaa aatgtatgaa tatttcaaac cactatttga atttatgcga agtacacctc
     7621 cactagcttt ggtgccaatg cttattttat ggtttggtat aggtgaagaa tctaaaatta
     7681 taattattat tttggcatcc ttttttccaa tatttttaaa tacattgaaa ggtataaaaa
     7741 attgtgacaa taaattaata gaagtaggta aatcttttaa tttatctaaa aagagaattt
     7801 tttataaaat aataatccct aattctatgc tggatattgt tgttggactt agtttagggc
     7861 ttgggtatag ttttagggct ataattggag cagagcttat tgcagcttct tcaggactag
     7921 gatatttgat ttctgatgga aaagatatgt ctagaactga tgttgtattg gtaggaattt
     7981 ttgttattgg atttttaggt atactttcag attatctatt ttcaaagata ataacaaaat
     8041 ttagcaaagg gaagcaggtt gatttaaatg gttagaagtg gatttttgct agagaatata
     8101 tataagaaat atttagttga taataaagag catttagttc ttgataatat atctttaaat
     8161 atatctagtg aagagataac tgttatatta ggtgaaagtg gatgtgggaa aacaacgcta
     8221 cttagaattt tagcagggct tgaaaatgct actagtggaa acatatattt ttttaataat
     8281 gataaaaagt gtactccaaa agtaggaatg gtatttcaag aaagtagact tatgccatgg
     8341 ctgaatgtaa gtgaaaatat acttttacat actgaaaaag ataatagaaa taaagttgat
     8401 ttagacaagt atttaaaaat gatgaagctt gaaaagttta aaaactcata tccaaatgaa
     8461 ttatcaggag gaatggctca cagagtaagt atagcaagag cactttcctt caatcccgat
     8521 attttgctga tggacgaacc atttgcagca ctagattact tcacaagaag aaaaatgcaa
     8581 aaagaagttg taaatatcca taaaaatact aaaaaaggtg tagtatttgt aactcacaat
     8641 attgaagaag ctatggaaat agcaaagaaa ataattgttt ttagtaaaaa taaaaggatt
     8701 aaacaattta gtgtcgaaga tgaatataat agagatttaa ctaaaaacta ttatataaac
     8761 ttgaaaaaag aaatattaag ggaattgggg gagttttaat tgattaaaag aaacaaaaag
     8821 attttagcag taatgacatc tttaataatg gtgttggttg ggactgtagg atgttcaaca
     8881 acaaatgata agaaagatga acctgcaaaa aaagaggcta gtttacctaa ggaaataaat
     8941 ttaacttatg taaaatcacc acttaatgtt cctagtatat tagaaaaaaa ggatgattta
     9001 tttggaaagg aatttaaaaa ggacaatata aaagtcaatt tccatgaatt gacaacagga
     9061 ccagagcaaa ctcaagcatt agcagcaggt gagctagact ttttacatgc attaggagga
     9121 acgtctgcaa taatagctgc atcaaatggt gttggtctta aaattacgaa tatttatagc
     9181 cgttcaccaa aaggatatat gcttataact aataatgata atattaaatc tataaaagac
     9241 ttaaaaggta aaaaaattgt aggtccaaaa gggactatac ttcatcaact gttgattgct
     9301 gcacttgcta aagaaggcta tactatagat gatgttgaat atgtaaatat ggatattcct
     9361 agtgctgctt ctgcattaga aagtaaaagt gctgatgtag caatgcttgc aggtccagtg
     9421 gcattgaaaa cgataaattc aggtgccaaa atgattgtaa atggagaagg attagtaagt
     9481 ggaataatag ttactgctgt aagtgatgac tttgctgaaa aatatcctga attagtaaaa
     9541 agatttatga aggttcatga agaaacttta aaatatatga atgaaaacaa ggatgaagtg
     9601 atggatgttg tatctaaaga agttggttta tctttagatg aaacaaaaga aatgtattct
     9661 tggtatgact ttagttctaa aataacggac aaggatataa aagaattaga agatacacag
     9721 gaatttttaa tgtctaatgg aatgcaacaa aagaaaataa atattaaaga tatgttgtac
     9781 aatcaaaatt agaatacttt aaaatattaa agtggaaaat acacccttat ttattatgga
     9841 aactatatgg taaaagaggg tgtattttta ttattataca aaaatacaat taaaaacagc
     9901 ttactatttt atctacactt atgtagtcta gtaattttct cattttctta cggtgaagta
     9961 aaaacttcga atatgtgtcc tcggattgtt ttgtgtcacc ataaactttc caatatttaa
    10021 tcatagaaac ttcataatta ctatatccca taaatccaat cacatcttta agaggatatc
    10081 ctaaaaaaat acctatttca tcaggaaaaa catcactctt aagtttatct actaaatgct
    10141 ctaaataagc attaacattc gtattttgtt tgtatcctaa aaactttaaa aagtttactg
    10201 tttttttaca ttttaatttt tctgataatg cttttttatt tacaaataag atttttaatc
    10261 cctttttttc tttatcaata actataaaat ctattttctt acatttatat agatatttat
    10321 aaatatcatt aattttttcc tctctattta catcagtaaa cgaaatattt attatttcag
    10381 caggtttaga gcctaaaaag actggaccta aaacttctaa aagccatttt atatatgatg
    10441 attcaatttt atttttacaa caaaattgtg aattacaaca agacataaaa gataacctcc
    10501 ttcaaatgaa aattaatatc aagtaattag attatactat gaatgataat caatatcaat
    10561 aataaaatta taattttttt atttaattat ttattcatta tgatttccaa aaaaataaat
    10621 tataac
//
LOCUS       DAMJVI010000117        10473 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.122, whole genome shotgun sequence.
ACCESSION   DAMJVI010000117 DAMJVI010000000
VERSION     DAMJVI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10473
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.122"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            159..776
                     /locus_tag="PC545_003131"
     CDS             159..776
                     /locus_tag="PC545_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDF2457874.1"
                     /translation="MEIFAIGHSNYPYDKLINMIKKYDINCVVDIRETPYSKYNIQYN
                     KEAFNESLRNSGFLYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLNGIERLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKVLNKEGFDVKYIMHEGNLAYQEDIEESLLD
                     KYFSDRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(933..1385)
                     /locus_tag="PC545_003132"
     CDS             complement(933..1385)
                     /locus_tag="PC545_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457875.1"
                     /translation="MVNLNANNLNVNMVYPISSTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTLGLGTMNIDKVYGQWAWFTDNIYDLNLFVFVGNYPYQIAKNRYDTFVDILPVA
                     ITSIVNGDRIFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1833..3191
                     /locus_tag="PC545_003133"
     CDS             1833..3191
                     /locus_tag="PC545_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2457876.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDGEKVNETIKQALVSGVILSFTITILM
                     WVFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIATQQIANGLLRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGIDLNPLGINLQTPSYGITGAAISIAIARIIGCIVIIRAL
                     FKGNEFISMGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFIV
                     GAIFIPIAPWISSMYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     VA"
     gene            3464..3844
                     /locus_tag="PC545_003134"
     CDS             3464..3844
                     /locus_tag="PC545_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2457877.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMRQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRMFSFKDQDGNDYLVREDK"
     gene            3969..4169
                     /locus_tag="PC545_003135"
     CDS             3969..4169
                     /locus_tag="PC545_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457878.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4365..5093
                     /locus_tag="PC545_003136"
     CDS             4365..5093
                     /locus_tag="PC545_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2457879.1"
                     /translation="MLEIKNLSFNVESNNEELGIINDVSLSFERGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESNQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIMCQVNSSCELMKDMN"
     gene            5115..6032
                     /locus_tag="PC545_003137"
     CDS             5115..6032
                     /locus_tag="PC545_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HDF2457880.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTETGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGEGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVILEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            6174..7358
                     /locus_tag="PC545_003138"
     CDS             6174..7358
                     /locus_tag="PC545_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2457881.1"
                     /translation="MKNLLIRNGTIISPTDGIKFEKRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNESGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKESGTLRKSVINAKNRGVLFDVCHGLNQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLSVINKIMELGISLEKCIEKVTEYPTKIFDLHGNKGRI
                     KIGGEGDFTIFTMEECNDIIKDYNNNELVLNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7465..7653
                     /locus_tag="PC545_003139"
     CDS             7465..7653
                     /locus_tag="PC545_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDF2457882.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7803..8453
                     /locus_tag="PC545_003140"
     CDS             7803..8453
                     /locus_tag="PC545_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2457883.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIKEDLENLD
                     QFIGPPLHDTFKEYYKFEDKKTKEAVEKYREYFADKGIFENKIYENIKEILEMLYKNG
                     KILLVATSKPTVFAETILRYFDIDRYFKYIAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYIVENVESIKDILL"
     gene            8556..9494
                     /locus_tag="PC545_003141"
     CDS             8556..9494
                     /locus_tag="PC545_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2457884.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYNGFK
                     EHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDNLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFIINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEIIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9520..10371
                     /locus_tag="PC545_003142"
     CDS             9520..10371
                     /locus_tag="PC545_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDF2457885.1"
                     /translation="MNSKIKYFLKFFILIFILIFIYSVAIEPSLLIVKKYNISENGTL
                     IKSDTIESKSERIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPSVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSNFNLLVNENKKIK
                     LKDDKYINILGVDEILNGNPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKDTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         4251 a          999 c         1855 g         3368 t
ORIGIN      
        1 tataaagttg tatatttttt taaggatata aagtaaaaaa atatacaata tcaagaaaaa
       61 aagatattgg gtatttaaag taatacttta tttaatttta agtaaatcgg gtatctacat
      121 atatagatac taatttcaaa gaataggagg gtgtttatat ggaaatattc gcaataggac
      181 attcaaatta tccatatgat aagcttatta atatgataaa aaaatatgac ataaattgtg
      241 tagttgacat tagagaaact ccatattcta aatataatat tcaatacaat aaagaggcat
      301 ttaacgaatc tcttagaaat tccggatttt tatatatata catgggaaaa gaatttggag
      361 ctaaaagaac taataaagat gtatatacgc aagaaggtta tgcagatttt gagaaagtag
      421 caaaagaaga tattttttta aacggaatag aaagattaaa aaagggttgt caaatgggtt
      481 ataggatagt tttattagga gctatgcaag aacctataag atgtcataga agtatcttgg
      541 tgggaaaagt attaaataaa gaaggttttg atgtaaaata tataatgcat gaaggaaatt
      601 tggcatatca ggaagatata gaggaatcat tattggataa gtattttagt gatagaaagc
      661 aactgagtat tgacaattta ttagggagtg cattaacgag agaagaaatg atacaagaag
      721 gttataagct agctaataaa gaaataggat atagaactga gaaactaggt aaataaaaat
      781 atacaagtag ataaaataca gtacttatct taagtaaaat aatattatga atttataata
      841 gctaggcatg atgtgccaat taaaaatatt ctagttattt aataatatca ttaaatttag
      901 ggtatttttt aatagcacat catgttgcag ttttaaaaaa taaaatctct aatattgcca
      961 taatcaactg ttctattaag agtaggatga ctagatataa atctaactgt aatttttgaa
     1021 tttaacaaac aaggatttga aattaaaaat attctatctc cattgacaat agaagtaata
     1081 gctactggca aaatatctac aaatgtatca tatctgtttt tagcaatttg ataaggataa
     1141 tttcctacaa aaacaaatag atttaaatca tatatattat cagtaaacca agcccattga
     1201 ccataaactt tgtcgatatt cattgtgcct aaaccaagtg tatcaaagta tgttgctata
     1261 gttaaataaa tattttccat gttttctaaa tatgaaacag tatatcttct gaaacgtatg
     1321 ggtgctgtgg aagaaatagg ataaaccata tttacattta gattatttgc atttaaatta
     1381 accaaagtat cacatcctaa aattagtcta taaaatacaa tatttaaatg gatgcgattt
     1441 tgttacatct aaagtgtaat attttatata ttgaaaaagt ggtaaaagtt tggatgatta
     1501 aataatatta taaaaaatat ataaaatgta cttattgtta ttgctacacg atagtgatac
     1561 aatattaacg aaatatagct acttttaata ataacggaaa atatttatat taaattaata
     1621 aatattttaa atgtggtatc cataaaaaat aataaacata agataattat aaagatatac
     1681 tgtgttcaaa tgatattaaa caagatttat attatttgaa agcagtatat tataatgcga
     1741 acaatacaat tctaaacttt gcattgtcaa tttacagttc gataaactaa ttaaaaatta
     1801 aatttatata aagagggaaa agaggaatag ggatgtttat aaggaaaaat gttataaagt
     1861 tagcaattcc tataatgata gagcagacgt ttgtcatgct tttaggagta tttaatacaa
     1921 tgatggcagg tcatattggg gaagaagctg tatctgcagt tggaatggtt gattcaataa
     1981 acaatatatt tatatctgta tttgctgcac tatctgttgg agctactgtt gttgtagctc
     2041 aacaaatagg aaaaaaagat ggtgaaaagg tgaatgaaac tattaagcag gctctagtgt
     2101 ctggagtaat attatccttt acaataacaa tattgatgtg ggtttttaga actggtgtaa
     2161 ttaatttatt ttatggttcc gcagaagaat tggtaaaatc aaatgcaaaa ttatacatag
     2221 agtttacatt atttacgtat ccatttattg cgacacaaca aatagcaaat ggtcttttaa
     2281 gagggtgtgg agatgtaaag aaacctatgt atgtgactat gtttatgaat atagtaaatg
     2341 taacattagg atatatactt atatatggta ttgatttaaa tcctctagga ataaatttgc
     2401 aaacaccatc ctatggcata acaggtgcag ctatatctat tgctatagct agaataattg
     2461 gatgtatagt aattataaga gctttattta aaggaaacga atttatatct atgggtaaaa
     2521 tatttccatt taagattgat gttgaaactc aaaagtctat atttaatata ggaataccag
     2581 ctggagttga acaactactt tttaatgcag gtaaattgat tgttcaagta tttatagtta
     2641 ctatgggaac atcagcaatt gcatccaatg caataggaat gtcaattgca tccataataa
     2701 atgtaattgg gaatgcttta gctttgtctg ctacaacctt ggtgggacag tatataggac
     2761 gtggagatat taaaggagct aagagtactt tattatattt aactaaattt gcaacaattt
     2821 gtttatttat agtaggagct atatttatac caattgcacc ttggatttca agtatgtata
     2881 ctcaaaatgc tagtgtaatt gacatctcaa cacaattaat tcgaagtgat tgtttagcta
     2941 tggtaatatg gcctatatcc tttgtattat cagcagggct aaagggagcc ggtgatacta
     3001 gatatacaat gatgactgca gtaataggta tgtggatttt tagaattttt acaggttacg
     3061 tattgggggt tgtattagaa tttggagtgt taggaatttg gataggtatg tatactgatt
     3121 ggcttgttag agggacttta tattgtttaa gattaagagg aacaaagtgg ctaaaacaca
     3181 aagttgcata gaatatatga aatcactttt agatggataa ctctatttag aagtgatttt
     3241 ttatgtagtt attatcataa ttataagtat gatatataag gtgattaaat tacataaata
     3301 aagcctataa tagtatggat actataaatt atatattgag cagaattaca taatttggcg
     3361 ataaatatta gaaatatata tctatagaaa ataatatata tgggtatatt attaaatata
     3421 aaaaatatta ggtatttatt taataataaa ggtgggtatt gttatgataa ataaaatagg
     3481 aaaaataact ctctatgtaa ataatcaaga ggatgcaaag ttgttttgga cagaaaaatt
     3541 gaactttgta gtaacttatg aaaaggaaat gggtccaaat atgaaatggg tggaagtagc
     3601 accaagtaaa aatgaattta caacatttgt gttgtatgat aaaaatctaa tgatgagaca
     3661 aaacaaagag ataaatgtat ctcatccatc aataatttta agtacaagag atattaatgg
     3721 aacttatgaa caaatggcaa ataacgaggt tgaggttggg gaattaatgg agatgccata
     3781 tggaaggatg ttttctttta aagaccaaga tggaaatgat tatttggtca gagaggataa
     3841 ataattttac aaagtattaa atttactcaa tatctgatat aattagagac aataattatt
     3901 ataaactagt ctaggattag aattatataa ttaaaaatta agtgtaaatt ttgagtagag
     3961 gtgataaaat gtttaagaaa atggcagtac taaaagatat agcaactaaa ataggtcgta
     4021 aaaaagctta tgaactatta gaaatggttg aaggtaatga tgcctttgta gctgaggtga
     4081 agataaaaaa gaatggaata gaatctaaaa aagaagaaat tatgttaaaa gataatcaaa
     4141 aaataatatt agagtatata gaaggctaat ttttaaatac atataagttg atatactaaa
     4201 tttctgagat gagtttacat aaaactcatc ttttttactt ttcaataact ttgttttatt
     4261 atttaatata cattttatct gcttagagtt gctgatatat aaaaattact gatataataa
     4321 aagaatggat attaatgtaa tattttatag aaaagagggg tattatgctt gaaataaaaa
     4381 atctttcctt taatgttgag tctaacaatg aggaactagg aataataaat gatgttagcc
     4441 tttcatttga gaggggaaaa cttattgtta tcacagggcc aaatggtgga ggaaaatcta
     4501 caattgctaa gcttattatg ggaattgaaa aagctacgag tggtcagata attttagatg
     4561 gagaagatat aacaaatctt agtattacag aaagagcaaa aaaaggaata ggatatgcat
     4621 ttcaacaacc accaagaata aaaggtatga cagttgaaaa tttacttaca cttgctcatg
     4681 gaaaaccatt gtctactgat gtatgttgcc aatatcttac agatgttgga ttgtgttcaa
     4741 aagactattt aaatcgtgaa gttgataact ctctttctgg aggggaaatg aaaagaattg
     4801 agatagcaac tctttttgct agagatttaa aggtttctat atttgatgaa cctgaagcag
     4861 ggatagacct ttggagcttt gggaaactca atgaaagttt taagaaaata catgaagagt
     4921 ctaaccagac tataatcata atttctcacc aagaaagaat attagagtta gctgatgaaa
     4981 taattgttct acaagatggt tcagtaaaaa gccatggaac aaaagaggct atattacctg
     5041 aaatcatgtg tcaagttaat tcaagttgtg aattaatgaa agacatgaat taattagaaa
     5101 ggaaggcaaa ttaaatgaac aaaatagatt taaatctttt agaaaaagtt gctgaacttc
     5161 gtggaactcc attaggagca tacaatatac gtaaaaatgg gcaaggagta gagagacatt
     5221 caactgaaaa tgtaactata attcctaaaa ctgataaacc tggaatagat attatagtaa
     5281 agccaaatac aaaaggagaa aatgtacata taccagtaat agtaacagag actggtgtac
     5341 aggatatggt atataatgat tttgaaattg gagaaaatgc agatgttgtt ataattgctg
     5401 ggtgtggtat acataactgt ggttgtgatg atagtgaaca taatggtata catagatttt
     5461 tcttacgtaa aggagctcgc ctaaaatatg tagaaaaaca ttatggtgaa ggtgatggag
     5521 aaggaaagag aattttaaat ccagttacta ttgtcaatat ggatgaggat tcatattgtg
     5581 aaatggaaac aattcaaatt aagggtgtag attcaactaa aagagaaatg gatgcaaacc
     5641 taggtaaagg agctaaaata gttgtatttg agaaattatt aactcatggt aatcaagttg
     5701 cagaatctaa tatgaaggtt atcttagagg gggaaaactc ttctgctcaa gtagtttctc
     5761 gtacagttgg gcaagataat tctaagcaaa agtttaatcc ttgtgttatt ggggattccc
     5821 tttgttctgc acatgttcaa tgtgattcaa taattatggg aaattctcaa attagtgcta
     5881 tacctgaaat tgctgcaaat catccagatg ctcttttagt acatgaagca gctattggaa
     5941 agattgcagg agatcaaata ctaaagatga tgacattagg tcttactgaa gaagaagccg
     6001 aacaacagat acttaattgc ttcttaaagt aaattaaaga ttttatttaa tttaatataa
     6061 ttaatatagt ataatatagt gaataagtct aaaatatttt tatttttagg cttattttct
     6121 ttatgtaaga aaagctatta gagaaattat attgtaatta ggatgtggaa taaatgaaaa
     6181 atttgcttat aagaaatggg acaataatat ctccaacaga tggtatcaaa tttgaaaaaa
     6241 gagatatctt ggttttacat gggaaaatat atgctattgg attgaacctt aaagagaaaa
     6301 taagaaaaga aaaattactt acaaataaag agttcgagat aataaatgcc gataatatgt
     6361 atatttcccc aggatttata gatgttcaca cccattgtta taagagaaaa ttacctacag
     6421 gtatggattc tgattcaatt ggaattgaaa aaggttctac agttatattt gatgcaggta
     6481 cagctggtcc tttaaactac tatgatttta aaaaaaagta tatggatgag tgtaaaacag
     6541 aagtatattc ttttttaaat gttacaaatg agggactaaa tatattgaat gaatcaactt
     6601 ctttagatgt tataaatttt gacaaggttg agtcagttgt agaaaaaaat atagaaaaga
     6661 taaagggtat aaaagtaaaa gcgagtaaat ttcaatctaa tgaaagtggt atagatataa
     6721 ttaagaaatc aaagcaagtt gccaaaaaat taaatttacc cttagtggta tatataggag
     6781 agtatccatc atatatagat gaagtaatga atattttggg aaaaggagat gtggttacac
     6841 cagtatatgg aaatagtaca aatggaatcc ttaaagaatc tggcacatta agaaaatcag
     6901 taataaatgc aaagaacaga ggagtattgt ttgatgtatg tcatggattg aaccaatttg
     6961 attttaaagt ttttaaaaaa gcaataaagc aaggtttaga gccagaccta ataagtactg
     7021 atatgcatat aaaaaacatg aaagacgtaa actattctct tttaagtgtt ataaataaaa
     7081 ttatggaact tgggatatcc ttagaaaagt gtatagaaaa agtaactgaa tacccaacaa
     7141 agattttcga tttacatgga aataaaggca gaataaagat tggtggagaa ggtgatttta
     7201 caatattcac aatggaggag tgtaatgata ttataaaaga ctacaataat aatgaacttg
     7261 ttttaaataa aaaattaaga ttgcaatata ctgtaaaatc atggatgaaa agcagtgaag
     7321 tttatagaca tggatacgaa aatactataa taaactaaac aatagtgata aaataataaa
     7381 tatatgttta aattggtata tattggataa atatctaagg aaacaacaat taaaaaataa
     7441 taattttagg ggaggtattt tagtatgaaa gcatttgttg ataaggatgt atgtgtagct
     7501 tgtggagcat gtataggaac atgtccagac gtttttagta tgagcgatga agggcattca
     7561 gtagcaattg aaggagaaat accagcagac ttacaagatt tatgtaaaga agccgaagaa
     7621 ggatgtccag tttcagctat aacagttaaa tagagctgtt taaatttatt tttaaaataa
     7681 atatataaga tttaattggg tacttaagct atagtattag aaatttctat tactatagct
     7741 ttttaaaata gataaactta atatataaga tattactttt tacaaatatg gaggactgaa
     7801 atatgaaaaa aaattatgaa atagtattat tcgatttaga tggaacactt acagacccta
     7861 aagaaggaat aacaaagtct attcagtatt ctttaaatag ctttggcata aaagaagatt
     7921 tggaaaactt agaccaattt ataggaccac cattacatga tacttttaag gagtattata
     7981 aatttgaaga taaaaaaact aaagaagctg ttgaaaagta tagagaatat tttgctgata
     8041 aagggatatt tgaaaataaa atctatgaga atataaaaga gatattagaa atgctctata
     8101 aaaatggtaa aatattatta gtagcaactt ctaagccaac agtttttgca gaaactatac
     8161 ttagatattt tgatatagat agatatttta aatatatagc aggtagcaat cttgatggta
     8221 caagagtgaa taaaaatgaa gttatacaat atgtactaga cctatgtaat gtaaaagata
     8281 aagataaagt tattatggtt ggagatagaa agtatgacat aattggagcc aaaaaaatag
     8341 gtatagattc tattggagtg ttgtatgggt atggttcttt tgaagaactt agtgaaagtg
     8401 aacctactta tattgtcgaa aatgtagagt ctataaaaga tattttgtta taaatatctt
     8461 ttatagacaa aaatattaca aaatggtgtc gcatatttaa aaatatgata ttatagttaa
     8521 ggaagtcatt taaaaaagag gaggatgcac ttaaaatgaa atctatttta attatgggtg
     8581 gaagcgactt cataggttct gcacttgcta agcgcttgat taaatgtggg tatcaaattg
     8641 atatacttac aaatggtaaa aaggaaatag attataatgg ttttaaagaa catctaattt
     8701 gtgatagaaa agttcgaaaa gatatggaaa acattatcac aggaagaaaa tatgattaca
     8761 tatatgatat gacagcatat acaaaagaag atgtaagtaa tctgattgat tttataagta
     8821 tggataactt aaaaaagtat atagtattgt cagcaggagc agtatataaa gatagtggaa
     8881 gaaatataaa agaagaaaat gagaaaggtg aaaatgaaaa ctggggaaaa tatgggttaa
     8941 ataaaaaaga ggctgaagat tttattataa atagccctat accatatata attattagac
     9001 caacttatat atatggtgaa aataataatt tatatagaga gtattatttc tttgaaaaaa
     9061 tagaaaaaaa tgaaaagata ccagtaccaa aaggaaaaca agttagtaat caatttatat
     9121 atattgggga tttggtaaag gtattggaaa gcataatgaa aaatcctcat gtaagagagg
     9181 cctataatgt gacaaatcca cagttaattt cttgggatga ccttatatat acatgtggag
     9241 aaattattgg caaagaacct ataataaaat atgtagatat ggaaaaagta gagtttagag
     9301 aaagaacgta tttcccattt agaaatatag actttaatct tgatataaat aagcttattg
     9361 aacatgggct ttatatacca aatgtgttac taaaagaagg tcttacagca acatataaat
     9421 ggttttcagc aaataagcca aaaatgcatg acagaaaaat gaacaaagtt gagtctgtaa
     9481 tgcaaatagt ttaaaaaaat aaataagtag gtagatattt tgaactcaaa aattaagtat
     9541 tttttaaaat tttttatctt aatatttatt ttgattttta tatactcagt tgcgatagag
     9601 ccgagtctat taattgtgaa aaaatataat ataagtgaaa atggaacatt gataaaaagt
     9661 gatacaattg agagtaaatc tgaaaggata aaagtagttc aaatttcaga tactcaaata
     9721 ggtagttttt attcaactaa gaatctcaaa aaagtagcta ataagattaa cactttgaat
     9781 ccagatataa tagtatttac tggtgattta attgattact ccaataaaaa tccaagtgta
     9841 gatgaaatta caactatttt atcaagtatg aatgctaggt tgggaaaatt ttcagtattt
     9901 ggcaatcatg attatatgta taaacttcct aggtattata ggcagattat gaaaaattcc
     9961 aacttcaatc ttttagtaaa tgaaaataaa aaaataaaat taaaagatga taaatatatt
    10021 aatatattag gtgttgatga aatattaaat ggaaatccta atataaaata tctggagagt
    10081 caaatagata ataagaattt taatttattg ttagcacatg aaccagattt agtagatatg
    10141 cttagtaaag atacaatgaa tttagttcta tctgggcata gtcatggtgg tcaaataaga
    10201 ttacctatta agggagcatt agttactcct ccatatggaa ggaaatatac taaagggttt
    10261 tatgatataa atggaaatca tttatatgtg agttcgggtc ttggaagtac aaagttacca
    10321 tttagatttt ttaatatacc agagataata gaatttaata ttataatcta acataaaata
    10381 tttagaaaat ataattttgc tttttataat atgtagatgt atatactgta aaaatggatt
    10441 aaaccagtag attgaatgaa ttaatagatt aaa
//
LOCUS       DAMJVI010000118        10426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.123, whole genome shotgun sequence.
ACCESSION   DAMJVI010000118 DAMJVI010000000
VERSION     DAMJVI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.123"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            248..1672
                     /locus_tag="PC545_003143"
     CDS             248..1672
                     /locus_tag="PC545_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDF2457886.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWVIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TMAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANESEEDSTSLEEAKAKMSDMKSASKNSEKNIEKKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLDIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDTLFKRLESK"
     gene            1707..3836
                     /locus_tag="PC545_003144"
     CDS             1707..3836
                     /locus_tag="PC545_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDF2457887.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGTLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEENIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFSLASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKAIEILSENRLLNLMDRCIIIK
                     VEATLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMETDFNMDIPVDEVGYITMHIRGAKQRSSSNHKALNLDDIEIMEITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYAYDGVE
                     KISRIIKDKLNINSIPESEIGYIAMHFASAIEKNLMMNTNINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKGKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QIIKEKIKSIAQNKLINLNTKNDTKSKNKVYEQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIMSIKESLKERLKISIPYIEESKILLLHCMSERIDIMK
                     LSIIKLENRIVLDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMTLEVKDIVFNFYTNRLKAFIDE"
     gene            3866..4288
                     /locus_tag="PC545_003145"
     CDS             3866..4288
                     /locus_tag="PC545_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDF2457888.1"
                     /translation="MSKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAK"
     gene            4307..5458
                     /locus_tag="PC545_003146"
     CDS             4307..5458
                     /locus_tag="PC545_003146"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDF2457889.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVQAEIITTAVGPLVLTKIASTIAK
                     GIKARKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEIYVGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFICDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYGLSVDNLILGIGAALHYN
                     NSEDTQSVELQELIKSNGVKKAVAKIANISNDEELLNNIEKSYIFMKNL"
     regulatory      5564..5665
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5848..6420
                     /locus_tag="PC545_003147"
     CDS             5848..6420
                     /locus_tag="PC545_003147"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDF2457890.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFKEEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANDVRLESLAVVESIDNGTVKFR"
     gene            6484..7134
                     /gene="fsa"
                     /locus_tag="PC545_003148"
     CDS             6484..7134
                     /gene="fsa"
                     /locus_tag="PC545_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDF2457891.1"
                     /translation="MIFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHEGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            7286..10210
                     /locus_tag="PC545_003149"
     CDS             7286..10210
                     /locus_tag="PC545_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDF2457892.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKI
                     GIKDKEPASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCKNEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSRIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTLG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKEHIEKQIKEYDTLIEVKPVAFLNNNLEEELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPVLNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
BASE COUNT         4274 a         1014 c         1952 g         3186 t
ORIGIN      
        1 ctgaattatt attttcattt ctattaaatt taaatatttt ataaaaatag tattatttac
       61 aaaatcatat actccaaaat actacataaa taaaattaaa caaaaactaa aatgacaaat
      121 gtcattatct aaagtgacat agaaacggtt tcttaaagtt gttactataa aatataaact
      181 ctataataaa agtataacaa aagggaatca tatcgatata gaatataatt caagaggagg
      241 taaaattatg agtagttcta atcaatcgac aataaagaca tctatacagc gagtagggaa
      301 atttttaagt ggtatggtaa tgccaaatat aggagctttt atagcatggg gtcttataac
      361 agcattattt ataccaacag gatggatgcc taatgaaaaa ttgagtacta taggcgaccc
      421 aatgataaaa taccttttac cactattgat agcttatact ggtggtaaag caatagcagg
      481 acaaagaggt ggcgtaatag gtgcagcagc agctatggga gtaattgttg gggcagatat
      541 accaatgttt atcggagcaa tgataatggg accatttgca ggatgggtaa ttaagaagtt
      601 cgataaattt gtagatggaa agataccaac aggatttgaa atgttagtaa ataacttttc
      661 aataggtata ataggtatgc ttcttgcaat actaggattt tatgcaattg gaccagcaat
      721 agtagcaggg acagctctta tagaatctgg agttcaattt atagttagca aaagtttatt
      781 gcctttagtt tcagtattta tagaacctgg aaaagtatta ttcttaaaca atgcaataaa
      841 tcatggtata ttagggccta taggtatagc tgaggcaaaa gaagcaggta aatctataat
      901 gttcttacta gaaagtaacc ctggaccagg tttaggagtt ctattagcat attggatgtt
      961 tagtagagga agtgtaaaac aatcagcacc aggagctgta ataatacatt tctttggtgg
     1021 tatccatgaa atttacttcc catatatttt aatgaatcca gttttaatat tggctacaat
     1081 ggcaggtgga gcagcaggaa tattgacttt ttcaatatta ggagctgggc ttgtagcagc
     1141 accatcacca ggaagtatat ttgcacttat ggcactagct ccaaagggag gattacttcc
     1201 agtattagca ggggttgcag tggcaacagt tgtatcattc ttagtggcag caccatttgt
     1261 taagagagct tcagcaaatg aaagtgaaga agacagtact tctttagaag aagctaaagc
     1321 aaaaatgagt gatatgaaat ctgcaagtaa gaactctgag aaaaatattg agaaaaaaca
     1381 gcttgaagta aatgagatta agaagattgt atttgcatgt gatgcaggaa tgggttcgag
     1441 tgcaatggga gcttctagat ttaaaaatag aataaaaaac ttagatttag atatagaaat
     1501 tacaaattca tctgttgata atcttccaga tgatacacaa atagtagtaa ctcacaatac
     1561 attagttgaa agagttgcta agaacaattc aagtgttgag atagtttcaa taaacaattt
     1621 tttaaatgac ccaaatttag atacattatt taaaagatta gaatctaaat aaaaataaaa
     1681 agtaccacta attaggagtg gataatttga aaaaaaagaa gattacatct aggcaaaaaa
     1741 agataatact aatgatagta gaaaattcaa agaaaaacat tccaataact atttctgaga
     1801 ttgcagggac gttagagtta agttcaagaa ctgtacttag agatatgtct ggaattgaaa
     1861 aatggtttga tgaaaatgat tttaactttg taaaaaaacc aggagtagga ttgattttag
     1921 aggaaaatat agaaaatcaa aactttataa ttgagttgtt agaagaagaa aaaatagaaa
     1981 aagaatacag caaggaagaa agaaatttaa ttatattatc aaagctacta gtttctaacg
     2041 agccagtaaa atcttattat tttgcaaaaa tattaaaggt atctgaaggg gtactgaata
     2101 atgatttttc attagcaagt aaatggttgg aaagatttga tattgagctt gttagaaaac
     2161 cagggcttgg tgtctaccta aaaggtcaag aaaaaagctt tagagaggca tatgtaaatc
     2221 ttatatacga ttcattcaat gaaaaagaaa tactagatat ggtcagaaat ataagtgaaa
     2281 atatacaaac agataaagca atagaaattt taagtgaaaa tagactttta aatttaatgg
     2341 ataggtgcat aataataaaa gttgaagcta cattaacaaa gaagctttca gatttagatg
     2401 taaatttagc tgatagtgca tatatagggc ttgtagttca tatttcatta gccttacaga
     2461 ggataaaaaa tggtgaaaat ataactatgg ataaagagtt tctaaaagaa ttatctataa
     2521 cagaagagtt taaattagct aaagaaatcg taaaaggtat ggaaacagat tttaacatgg
     2581 atataccagt agatgaggtt ggatatataa ctatgcatat aagaggtgca aagcaaaggt
     2641 catcttcaaa tcataaggct ttaaacttgg atgatataga aataatggaa attacaaata
     2701 agatgataga tttggctgaa gatgaattta aaataagttt aaaaaatgac gagaggttat
     2761 ttaaagacct agcaaatcac ttaggacctt ctataaatag attgaatatg ggattagaaa
     2821 taagaaatcc tcttttagat gaaataaaaa gcaagtattc atatgcctat gatggagttg
     2881 aaaaaatttc tagaattata aaagataaac taaatataaa ttcaatacct gaatcagaaa
     2941 taggatatat tgcaatgcac tttgcatcag ccatagaaaa aaatcttatg atgaatacta
     3001 atataaatat tgttgtggca tgtcccactg gaataggtac ttcaagattc ttatcaacta
     3061 agattgaaaa taaatttcct aatttaaata tacttgaaac catatcagct ataaacatag
     3121 atgaagagta tttaaaggga aaagatgttg atttaatagt gtctacagtt gagttaaata
     3181 caagtttaaa ttatatatgt gtaggtccat ttatgagttt agatgatgaa cagataatca
     3241 aggaaaaaat aaaatctata gctcaaaata aattaattaa tttaaataca aaaaatgata
     3301 ctaaaagtaa gaataaagta tatgaacaga taacagaaag tatgaatata ggaaaagata
     3361 ttcttcaatt tttagaggaa attagatttg aaaaatttga aagtaaagat ttaagtgagc
     3421 ttattgaaga ctcatcaaaa atatttgcaa aatctagtga agatataatg tctataaaag
     3481 aatcgttaaa agaaagatta aaaatctcaa ttccatatat agaagaatca aaaattctat
     3541 tattacattg tatgagtgaa agaatagata ttatgaagtt atcaataata aaactggaga
     3601 atcgaatcgt gctagattca aatgaagaaa tcgacaatgt agtttttatg ttacttccta
     3661 aaaattcacc aagttatcaa agacaaataa tgagtgaaat aagtggttca ttaatagaca
     3721 atataatttt tactaataag ataaataaat tctctataga agagatgaca ttagaggtaa
     3781 aagatatagt ttttaatttt tatacaaaca gattaaaagc atttatagat gaataaaatt
     3841 aatattaaaa aggaaggttg atactatgag taaagtattg aatgagaata atatattttt
     3901 agggttagat agtgtttcaa aggaagaagc tataacttta gcaggaagaa aattagttga
     3961 aaatggatat gtaaaagaag aatatatacc agcaatgcta gaaagagaaa aagtaatgac
     4021 tacatatatg ggaatgggag ttgctattcc acatggtgta aatgaagcaa aaaaagaaat
     4081 attatcttct ggaatagtta tactacaatt ccctaatggt atagattttg atggagaaaa
     4141 agcatactta ttaataggaa tagctggtgt tggagatgaa catttagaaa tactatctaa
     4201 tatagcagta gttttagatg atgacttaac agaaagacta aaaaattcaa atgacaaaca
     4261 agcttttatg gaagcttttg ccaaataatc ttaggaggga tttaaaatga aaaaggcaat
     4321 ccagtttgga gcaggaaata ttggaagagg ttttattgga ggcttgttag ttaagtcggg
     4381 ttatcatgta gtatttgctg atgtaaatga ggagatatta aactcaataa ataaagataa
     4441 aaagtatact atacacataa gagatgtaga atgtatagat gaagtaattg ataatataag
     4501 tgcggtttca tctataaaag aagaaataat agatgagatt gtacaggctg aaataataac
     4561 tacagcagtt ggacctttag ttttgactaa aatagcctct acaattgcaa aaggtataaa
     4621 ggctagaaaa gaaaagggtt taacaagtaa tctaaatata atagcctgtg aaaatgcaat
     4681 atacgcaagt tcttctttaa aagaagaggt acttaagtat ttaaataagg aagaagtaga
     4741 atatttagag atatatgttg gttttccaaa ctgttcagtt gatagaatag ttcctcctgg
     4801 taaaaatgaa aatcctcttg atgtaacagt tgaaaatttt tatgagtgga atgttgaaaa
     4861 acaaggattt aaaggggaaa taccaactat agttggaatg aatttagctg acaaccttat
     4921 ggcttatata gagcgtaaat tgtttacact gaatacaggt catgctataa cagcttatat
     4981 aggatattta aaaggatata agactataga agagagtata aaagataaat ttatctgtga
     5041 tatagtcaaa agtgcaatgg tagaaagtgg agaaggattg attaaaaaat acaattttga
     5101 ctcagaagcg cactataaat atattgataa aattttaaat agatttaaaa atccatatct
     5161 taatgatgat gtattaagag ttggaaggga accattaaga aaactaagtg ataaagatag
     5221 attgattaaa cctttaatga cagcaaaatc atatggatta tcagttgaca atttgattct
     5281 tggaataggt gcagcgcttc attataataa tagtgaagat actcaaagtg tagaattaca
     5341 agagttgata aagtctaatg gtgtaaaaaa agctgttgct aaaatagcta atataagcaa
     5401 tgacgaagaa cttttaaata atatcgaaaa aagttatata tttatgaaaa atttataaat
     5461 ttacttgaaa aaaagtagtt ctgataaagt aactataata tattattcta cacagtttga
     5521 attatttgtt gaataataga gaattataat atataattaa aaggaataaa ttaatatatt
     5581 tacctgtata tgtttgataa tatggttcaa atgtttctac taagctacca ttaatagctt
     5641 gactacagga gtgaagaaga tatagtttat acttatttta aattatatat taagttaatt
     5701 actttgaatt ttaagctcct gtctatgata tagacaggag cttttttagt attttaatat
     5761 aaaatactta caagaataga tagcaaaatt agaacaaata taattttttt attaatataa
     5821 ctttagaaag ggctaggtgg aagttcaatg aaggctttac aagaaaagat attgagagaa
     5881 ggcagtgttt caggaaatga tatactaaaa gttgatagtt ttttaaacca tcaaattgat
     5941 gtagcttttt taaatgaaat aggaagagaa tttaaagaga gatttaaaga agaaaaggtg
     6001 gataagatat ttacaataga ggcttcagga atagctatag catcaattgt ttcacagtat
     6061 tttgataatg cacctgtagt atttgcaaaa aaatcagaat ctaaaaattt ggataaagat
     6121 gtatatgaga ctaatgtata ttcatttact aaggcaagag aatattcagt taaagtttct
     6181 aaaaagtaca taaacaaagg tgaaaatata ttgatagtgg atgatttttt ggcaaatgga
     6241 agagctgctc ttggacttaa agatttaatt gagcaagcag aagctaattt agttggagta
     6301 ggaattgtca ttgaaaaagg atttcaagct ggaggagcac ttttaaaggc taatgatgta
     6361 agattagagt ctttggcggt agttgaatct atagataatg gaactgtaaa atttaggtaa
     6421 tttatgatat tatatacttg tgtttagcac atattttaac tatgtaatta tggaggattt
     6481 tttatgatat tttttataga cacagccaat attgaagaaa taaaagaagc aaatgattta
     6541 ggtgtaatat gtggtgtaac tactaatcca tctctaatag ctaaagaagg tagagatttt
     6601 atagaggtag ttaaagaaat aagtgaaata gtagatggac caataagtgc agaagtaata
     6661 agtttagagc atgaaggtat gatagaggaa gcagaaaaat tatctaagat acataaaaat
     6721 atagttataa agatacctat gacagcagaa ggtctaaaag cggtgaaagt actatcaagt
     6781 aaaggtataa aaactaatgt aacacttata ttctctgctg gacaagctct tttagctgct
     6841 agagctggag ctacatatgt aagtcctttt gttggtagat tggatgatat atcacaaaat
     6901 ggtcttgatt taattgaaga aatcgttgat atatttagtg taaatagtat tgaggctcaa
     6961 ataatagttg ctagtgttag aaatccaata catgttttac aagctgctag aatgggagca
     7021 gatatagcaa cagttcctct taaagttata aaccaaatga ttaagcatcc tttgacagac
     7081 aaaggaatag atagctttat gaaggattgg gaaggtgctt ctttaaaatt ataattataa
     7141 actacgcttt ataagtatat taaagaggtt attatggaaa ttctaagtaa taaggctagg
     7201 ctattttgcc tagtcttatt ttggtgtata atatattgaa acatattatt gaaatagatt
     7261 aattttttaa gtgaggaggc gaaaaatgga taaaatatta agagagatac aaagagaaga
     7321 taaaaaaaat ccatatactg accaagaact tgctgaaatg ttaaatgtag ctaggtctga
     7381 agtgatatca gtgaggaaaa aaaataatat tttagattcg agagaaagaa gaaaaaaaat
     7441 attaataaaa gatatatcaa aaattgtaag tgaaaatcct caaatgtctg aaagaaaaat
     7501 aactgagata ttaatagaga atggatataa tatatcgaga agtgcagttt caaaattatt
     7561 aaaagaagaa aatttaaatc agtcaaagat tgggattaaa gataaagagc cagcatcatc
     7621 aggaaagtat gtgaaggtga gtaaagttgt agaagaagat ggatttgaag aattgatagg
     7681 tataaaggga agtttgaaag aaaaggtaaa tctagctaag tcagcaataa tgtatcctcc
     7741 taatggcctt catactataa tatatggaga aacaggggta ggtaaaagtg aattagctac
     7801 atgtatgtat aaatatgcta taaaaaacaa catcaaagaa gagaacagcc catttatagt
     7861 gtttaactgt gcagactatg cagaaaatcc taatctattg atagctcaac tctttggggt
     7921 agtaaaaggt gcatatacag gagctgatgc aaatagagaa ggtcttgtag aacaagctaa
     7981 taatggaata ttgtttttag atgaaattca tagattgcct gctgctgggc aagaaatctt
     8041 attttcatta atagatagag gagaatttag aaggttagga gaaagtagtt ctaatagaaa
     8101 ggcaaatgtt ctcataataa gtgctacaac agaaaaccca gattctaatt tacttcaaac
     8161 atttagaaga cgtataccaa tggtaataaa tttacctaat atgagtgaaa gaccaaaatc
     8221 tgaaagatat gaaataataa taaagttttt tgaaagagaa gcaaagcgag taaacaagaa
     8281 ttttataata actaaagaag ttatgtgtgc acttatgaat tataagtgta ctggaaatat
     8341 aggtcagtta aagagtgata tacaagtaac ttgtgctaga gcttttagta aatcaatatt
     8401 ttctagcgat aaggtaatta tagatttaga tagtttaagg gattatataa aaagtggata
     8461 ttatgattgt aattgtaaaa atgaaaagta ttctgagttt tcagttgagg atatatatat
     8521 agatatcagt gaattggtga gtggaaaaaa tagaatttta tctgataatg agataagcga
     8581 gatatataat tatgcagaaa aagagcttaa ggttttggaa agtaagtgtt tttcagaaga
     8641 agagttaaaa aatgcattta tagaaaagtt agataagaag tttaatgcta ttaaaaacaa
     8701 taaaaattta tctgatagaa gaagtcgaat agactgggga ggacaattaa aagagtctac
     8761 attggagata atggataagg taattgtatt tattaaggag cagtttaagc atgtaaatca
     8821 aggattgtac ttggctctag caattcacat tgagcatgct ataggtagga taaaagatgg
     8881 aaaaactata ataaatccga gtttagataa aataaaagca tcaatgccaa tggaatatga
     8941 attgtcaaga tatataatgg gtatagtgga agatttgaca caagtaacat ttccagaaga
     9001 tgaacttgga tatttggctt attatataaa taaattttgt tataatgagg aaagtataaa
     9061 agataaagtt aaagtagtaa tagttacgca tggtaaagtt ggaattgaaa tgtctaaagt
     9121 tgtaaatcat attttaggta tagaatgtac actaggtata gaaatagcac ttacagattc
     9181 accatcagag gggatagagc atgtattaga agagcttcaa aagatagaag ctagaaaagg
     9241 tattctagtt ctaattgata tgggttcttt ggtaattctt ggtgatgagg ttgaaaaaag
     9301 acttggaatt agatgtaaaa ctgtaaacag agtagatacg ttattggcaa tggaagcagg
     9361 aaaacttgca accattgaag gaaaaagttt agatggtata atagcagatt taaagaaaaa
     9421 taagaattat gccatgataa atacaaataa atttagttac agaaaaaacg agtatggaaa
     9481 gaaaaatgta atcattactt tatgtttgag tggtgtaggt acagctttaa atttaaaaga
     9541 gcatatagaa aaacaaatta aggaatatga cactttaatt gaggtaaagc cagttgcttt
     9601 tttaaataat aatttagaag aagagttaaa tgatattgaa gctgaatata atattattgc
     9661 tatctgtgga actatcgata tagattataa aaacgtacct tttatttctt ataatgaggt
     9721 acttggtaaa aatggtatta ataaagtatt agagcattta aacagtaaac aaccagtttt
     9781 aaatattgaa aaccagttga ataatttaat acatgaagat ttgattttat acaattttga
     9841 aggaatttca aaggagtata taattgatac tcttgtttca aagcttgaag aaggtggata
     9901 tgttgactca aagtatatat taagtgtata taaaagagaa gcaatgggaa gtgttgtaat
     9961 ggcctctaag gtagccgtgc cacatggact tccagaaaac gttataaagc ctgcaatagc
    10021 aatcgcaagg ttgaataagc ctatagtgtg ggacaataaa tttatggtgg atttggtagt
    10081 actactggca cttaaagaaa ataataagaa agaaatacga agcttatttt caaagattaa
    10141 tgaccaacat actttagaag ttttattaaa tactgaagat aaaaatgaaa taaaagaact
    10201 tttaagctaa ttattagtac agagaagaaa gacaaaaaat ttgctttagt ataaaattta
    10261 aaactactta attgctaagt agttaattac tcgatataag gctagttcta tgaaatttat
    10321 ttatatgatt ttatagaatt agcttttttc ttggtaaata aaatatatga atttatttat
    10381 gcaaaaagta aattttattg gacaattcta tgagaatttt tcctat
//
LOCUS       DAMJVI010000119        10406 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.124, whole genome shotgun sequence.
ACCESSION   DAMJVI010000119 DAMJVI010000000
VERSION     DAMJVI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.124"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..1032
                     /locus_tag="PC545_003150"
     CDS             <1..1032
                     /locus_tag="PC545_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HDF2457893.1"
                     /translation="SKKIKKLFLDDKGYYFNSNWKDIHIGIYNTKLGDSLKYITNEYI
                     ENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKNQTEELCLEAIKYNYKALE
                     YVKEQTEYICLEAIKKDCNALKYVRNKTERLIIKVIRHSSNIDVVSILKALETQTRRI
                     CLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIESVRQNGMALQY
                     VNKQTDKICIEAVKQDGRSLQFVNNKTEEICINAIRYLNKKYNIKDVLSYIDKYTEDI
                     CIEIVRQNGKMLMYIKNQTEKMCIEAVKENYKSLKYVKEQSERICKEALKQNHKAKEY
                     VKIAIDDCI"
     gene            1171..1623
                     /gene="tsaE"
                     /locus_tag="PC545_003151"
     CDS             1171..1623
                     /gene="tsaE"
                     /locus_tag="PC545_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDF2457894.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIEDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDDGREMILTPKGEFYKEIVEELIK"
     gene            1623..2339
                     /gene="tsaB"
                     /locus_tag="PC545_003152"
     CDS             1623..2339
                     /gene="tsaB"
                     /locus_tag="PC545_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDF2457895.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTVNNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     INFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVVEFDALVDEIVSTNEEFII
                     VGEAVYKYKDKLENIKNIKIPSPANNVSNAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            2329..2805
                     /gene="rimI"
                     /locus_tag="PC545_003153"
     CDS             2329..2805
                     /gene="rimI"
                     /locus_tag="PC545_003153"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDF2457896.1"
                     /translation="MEDNTKLIVDDIKIEEMTTEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLNGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            2811..3827
                     /gene="tsaD"
                     /locus_tag="PC545_003154"
     CDS             2811..3827
                     /gene="tsaD"
                     /locus_tag="PC545_003154"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDF2457897.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANIGFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAKKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKEKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            4124..6832
                     /gene="hpdB"
                     /locus_tag="PC545_003155"
     CDS             4124..6832
                     /gene="hpdB"
                     /locus_tag="PC545_003155"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HDF2457898.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLSEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKSAFSHMTPNIIPGEKIVMQ
                     KTRHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKAVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLKEDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDHRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATADGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            6836..7093
                     /gene="hpdC"
                     /locus_tag="PC545_003156"
     CDS             6836..7093
                     /gene="hpdC"
                     /locus_tag="PC545_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HDF2457899.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDAACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            7086..8036
                     /gene="hpdA"
                     /locus_tag="PC545_003157"
     CDS             7086..8036
                     /gene="hpdA"
                     /locus_tag="PC545_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HDF2457900.1"
                     /translation="MSSQKQLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKPYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSNEVFNKIFNDIDFAFIDIKHMDREKHKEQTGVYNDLILENIS
                     NLANSDWNGRLVLRVPVISGFNDSDENISDIISFMHKNNLVEINLLPFHRLGESKWIQ
                     LGKEYEYSDKGDVDEGHLGELQDIFLDNGIACYVGHETAF"
     gene            8351..9313
                     /locus_tag="PC545_003158"
                     /pseudo
     CDS             8351..9313
                     /locus_tag="PC545_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(9412..10392)
                     /locus_tag="PC545_003159"
     CDS             complement(9412..10392)
                     /locus_tag="PC545_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2457901.1"
                     /translation="MNLKLIWINILNPILIKNEIKRKKVQIIIFFFINFGLTYSLGLL
                     LYYNKFIDPENFASFMMILPLSSVAIAKFYTEGLNNDMYEFYSLIILFFLTYSLLIVL
                     KLLNLINNNEFIIINLILTFISSLSIIIYSSNKKNLYPIKNIKLGSLLIIYFMISVIV
                     PTSFILILQGSQPNYSGIIKFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFNKFGKKL
                     GVIILGICWSLWHLPLIFTLYTPETPILGLILRSIHIVGISIFLGYLYIKTKNIWFCA
                     IIHILNNSAFLITNSKESTITYHDILIVSMVVLIFYVPFLFTKEYKNNLE"
BASE COUNT         4087 a         1070 c         1901 g         3348 t
ORIGIN      
        1 tctaaaaaga taaaaaaatt atttttagat gataaagggt attattttaa tagtaattgg
       61 aaagatattc atattggtat ttataataca aaattaggag attctttaaa gtatatcact
      121 aatgaatata ttgaaaatct ggaaagggta aaatatgact atcgtgaatt aaaaaatata
      181 gaaaatcaaa ctgatggtat gtgtatttta gcaataaggc agaattacaa ggcattagag
      241 tatgtaaaaa atcaaaccga agaattatgt ttagaagcaa taaagtataa ttataaagca
      301 cttgaatatg taaaagagca aacagaatat atatgtcttg aggctataaa aaaggattgt
      361 aatgcactta agtatgtacg aaataaaaca gaaaggctta ttataaaagt aatacgtcat
      421 tcttccaata tagatgttgt aagtatacta aaggctttag agactcaaac aagaagaata
      481 tgtttagaag caattaaaaa agatggcagg tgtcttgcat acgttagaga acagagtgaa
      541 gaattatgta tagaggccat taaacaaaat tataaagcat taaaatatgt agaaaatcaa
      601 acggaaaaaa tgtgtataga atccgtaaga caaaatggta tggcattaca atatgtaaat
      661 aaacaaactg ataaaatatg tatagaagct gtcaaacaag atggaagaag tctacaattt
      721 gtcaataata agacagaaga aatatgtata aatgctataa gatatttaaa taaaaaatat
      781 aatattaaag atgtactaag ttacatagat aagtatacag aagatatatg tatagagatt
      841 gtaagacaaa atggaaaaat gcttatgtat ataaaaaatc aaacagaaaa aatgtgtatt
      901 gaagcagtaa aagagaacta taagtctctt aagtatgtaa aagagcaatc tgagagaata
      961 tgtaaagaag cattgaagca aaaccataaa gcaaaagagt atgtaaaaat tgctattgat
     1021 gattgtatat aaggtgtaga tacagccatc aatagtgaaa gataaaaaaa gataaagaca
     1081 agtactttat aataatctag gcttatacta taattaatta agcagtgata taaattcaca
     1141 tgacgattat atcaaaatgg agatgaattg atggcaaaaa tatatttaga aaatgaaaat
     1201 aaaactagag aaataggata taaattaggt aaacttttaa aagaaggcag tgttatctgt
     1261 ttagtaggag atttaggagc tggtaaaact acaatgacac aaagcttggc agattcatta
     1321 gggatagaag attatataac aagtcctact tttactataa taaatgaata tgaaggaaaa
     1381 atacctttgt atcattttga tgtttataga ataggtagta gtgacgaaat gtatgatatt
     1441 ggttatgatg aatatgtaaa ttcaaatgga atctgtataa tagaatgggc taatctaata
     1501 gaagatatac ttccaaaaga atatttgaat atagaattga gatataaaga tgacggaaga
     1561 gaaatgatat taactccaaa aggagagttt tataaagaga tagttgagga gttgattaaa
     1621 taatgaaaat actaggtatg gatacgtctt ctatggcagc aagtgtagca gttgttgaag
     1681 atgataactt aatatgtgag tttacagtaa acaacaaaaa aactcattca caaaagctta
     1741 tgccaatgat agaaaatatg ttgagtatga gtgatttaag tataaaagat atggatttat
     1801 tagctgtatg tataggtcca ggctcgttta ctggacttag aataggtatg gccacagtaa
     1861 aagctatggc tcatgtaaat aatataccaa taatagcagt aaattctttg gaatctctag
     1921 caaataacat aaatttctgt gatagaaaga tatgttgtat attagatgcg cagaaaaatc
     1981 aagtttattc agctaaatac aagtttgaaa atggagagat gatagaatta gatggtgtag
     2041 atgttgtaga gtttgatgct ttagtagatg aaattgtatc aactaatgaa gaatttataa
     2101 tagttggaga agctgtatac aaatataaag ataagttaga gaatataaaa aatataaaaa
     2161 taccttcacc agctaataat gtatctaacg caggaagctt atgctcttta gcattaaata
     2221 aatataataa aaatatagat gtacatactt gttatacgat aaatcctatg tatatcagaa
     2281 aatctcaagc tgaagtacaa tatgatgaaa aaatgaagag gctaaataat ggaagataat
     2341 acaaaactaa tagtagatga tataaaaata gaagaaatga ctacagaaga tatagatgaa
     2401 gtttttgaag tagaaaaaaa ttgttttgaa gattattggt caaaagagtc ttttagaaaa
     2461 gagttgtcta atgaagtagc aaggtatata gtagctaagt taaatggaaa aattgtcgga
     2521 tatgtgggaa tttggcttat acttgatgaa gggcatataa ataatgtggc tgttcatagt
     2581 gactatagag ggaaaaaaat aggggacaag ttgataaaag gcatagttga tttatgtaaa
     2641 gataataata ttgcatctat gacattagaa gttagagcat caaataaaat agcacaaaat
     2701 ttatatagaa aatatggatt taaaatgggt ggaataagaa aagaatacta cagtgataat
     2761 aaagaagatg caattataat gtggaaccaa ttaaaagagg tgtagagttt atgagtgata
     2821 taataacatt agctatagag agtagttgtg acgaaacagc tgcatcagtt ttaaaaaatg
     2881 gtagagaagt actttcaaac ataatctcta ctcaaataga gacacataaa aaatttggtg
     2941 gtgtagttcc agaagtggct tcaagaaaac atgtggaaaa tattgatata gttgttcaag
     3001 aagctttaga caaagcaaat ataggtttta atgatataga ccatatcgca gttacatatg
     3061 gacctggact agtaggtgct ttattggttg gtctatctta tgctaaagca cttgcatata
     3121 ctttgaatat tccacttgta ggagtaaatc atatagaagg gcatctaagt gcaaactata
     3181 tagagcataa ggatttgaaa cctcctttta taacattaat agtatctgga ggtcatactc
     3241 acttggttga agtaaaagat tatggcaagt atgaaatttt aggaaaaaca agagatgatg
     3301 catctggaga agcattcgat aaaatttcaa gagcaatgaa tttaggttat ccaggaggac
     3361 ctattattga taatctagcc aagaagggaa ataaacatgc tatagagttt ccaagagcct
     3421 atttagaaga agatagttat gattttagtt ttagtggatt aaaatcttct gtattgaatt
     3481 atctaaatgg gaaaagaatg aagaatgaag aaatagtagt tgaagatgta gcagcatctt
     3541 ttcaagaggc tgtggtagag gttttatcta ctaaggcact aaaagcagta aaagaaaaag
     3601 gatacaatat cataactctt tctggtggag ttgcttctaa ttcggggctt agagctaaaa
     3661 taacagagct tgctaaagac agtggtatta ctgttaagta tcctcctcta attttatgca
     3721 cagataatgc agctatgata gggtgtgcag gatattataa ttttataaac gggaaaacgc
     3781 atgatatgag tttaaatgca gttccaaatt taaagataaa tcagtagagt tagataaatt
     3841 tgtaaatata aaagtattgt ttcaaaaaag taagcagaag tgaaattaaa tataataaaa
     3901 atcaatatag tatatattaa aacttgaagt aaaactattt ataatcttta tattccaatg
     3961 aattgcaaca cattttagga tatacaggtt gtctttggct tagatttgct tcaagtttaa
     4021 ttttttctat agaaagatta ttttaaaaag tgggtaatat accctttttt ttcattctta
     4081 aaaagtttta tactaattat agaaagatta aggaggggaa gaaatgagtc aaagtaaaga
     4141 agacaaaata agaagtattt tggaagcaaa aaatatcaaa tcaaattttc aaaataaaga
     4201 aaatttaagt gaatttaatg aaaaaaaagc aagtaaaaga gcagaagact tattagatgt
     4261 atattataat acattatcta ctgcagatat ggaatttcca tattggtata atagagaata
     4321 tagaaaatca gatggtgata taccagtagt tagaagagct aaagctctta aatctgcttt
     4381 ttcacatatg acaccaaata taatacctgg tgagaaaata gtaatgcaaa agacacgcca
     4441 ttatagaggt tcttttccaa tgccatgggt aagtgaaagt ttctttgttg ctcaaggaga
     4501 acaaatgaga gaagaggcaa agaaattagc cagtaacaca gctgatgaac ttacaaaatt
     4561 tgggtctggc ggaggaaatg ttactgaaag ttttggaaat gtcgtttcaa tagctggtaa
     4621 attcggaatg agaaaagaag aagtacctgt acttgtaaaa atggcaaaag aatgggttgg
     4681 aaagtctgtt gaagatttag gttttcacta tgaaaaaatg atgccagatt atgatttaaa
     4741 agaaaaccta atgagtacac ttatatgtat gtttgattca ggatatacac ttcctcaagg
     4801 tagagaagtt ataaattatt tttatccttt aaattatgga ttagatggaa ttatagaaat
     4861 ggcaaaagaa tgtaaaaagg cagttgcagg aaatgcatct ggagatgggc ttattggtat
     4921 ggaccgtcta tatttttatg aggctgttat tcaagttatt gaaggattac aaacttggat
     4981 tttaaattat gcaaaacatg caaaatattt agaaagtata gagacagatt tagaagctaa
     5041 aaaagaatac tcagatttag tggagatatt agaacatatt gcacataaac aacctcgtac
     5101 atttagggaa gctcttcagt tgacttatac aattcatata gcatcagtaa atgaggatgc
     5161 tatatctggt atgtctatag gccgttttgg tcaaatttta tatccttggt atgaacaaga
     5221 tatagaaaaa ggattaatta caaaagaaga ggttatcgaa ttattagagc tttatagaat
     5281 aaaaatcact tgtattgact gttttgcttc agcgggcgta aatggaggag tactatcagg
     5341 aaatacattt aatactttat ctataggtgg attaaaagaa gatggttcta caggagctaa
     5401 tgaactagag gagttattat tagaagcaag tatgagatgt agaactcctc aacctagttt
     5461 aactatgtta tatgatgaaa aacttccaga agatttctta atgaaagctg ctgaatgtac
     5521 aaagcttggt tctggttatc cagcctgggt aaacaactca aatggaacaa catttatgat
     5581 gaaacaattt gctgatgaag gtatgactgt tgaagaagcg agagcatttg cattaggagg
     5641 atgtttggaa acgtctccag ggtgctggaa acagttaact ttaaacggga agacatactc
     5701 tattgcaggt ggagcaggtc aatctgctgg ctctggagtt cactttatag ctaatcccaa
     5761 aattttagaa cttgttctta tgaatggtaa agaccatcgt atgaatatac aagtatttga
     5821 gcctcataat aagccattag atacttatga agaagtcata gaagtattta aagattatta
     5881 taaacaagca ataaatgttt tagaaagagc aaataacatt gagttagata tatggagaaa
     5941 atttgatact tctataataa attcactatt aaagccagac tgtttagaca aaggtcaaca
     6001 tataggaaat atgggatatc gttataatgc aacattaaac gtagaaactt gtggtactgt
     6061 tactatggtc aattcatttg cagcacttaa gaaacttgta tatgatgaca aagcatttac
     6121 aatagaggaa atgaaggatg ccatattaaa taattttgga tttaaagatg cattagaagt
     6181 aggaaattat tctatggcag accaagtaaa agtagataag actggtaaat atgatgctat
     6241 ttataaggcc tgtcttgatg cacctaaata tggaaataat gatttgtatg cagataatat
     6301 tcttaaaaac tatgaggtat ggttatcaaa agtatgtgaa gaagcacaat ctttatatgc
     6361 taagaagatg tacccttgcc aaatatctgt ttctacacat ggaccacagg gagctgctac
     6421 cttggctact gcagatggta gactaagtgg aacaacatat tcagatggtt cagtctctgc
     6481 atatgcagga acagataaaa atggggtata tgcattattt gaatctgcaa ctatttggga
     6541 ccaagcagta gttcaaaatt ctcagatgaa tttaaaactt catccaacaa ctataaaagg
     6601 gcaacaagga actaaaaaat tattagattt aacaagaagt tatttaagaa aaggtgggtt
     6661 ccatattcaa tataatgttg tagattcaga aacattaaaa gatgcacaaa aaaatcctga
     6721 caattatcgt caattaatgg tacgtgttgc tgggtttact caatattggt gtgaattagg
     6781 taaaccaata caagatgaag taattgctag aacagagtat gaaggggtgt aaaatatgag
     6841 aaagcatagt gattgtatga atttttgtgc agtagatgca accaaaggaa tttgtagatt
     6901 atcaaaacaa atgattaatt tagatgatgc agcatgtcca gagataaaag taatgccaaa
     6961 atgtaaaaat tgtaaaaatt ttgttgaagc taatgatgaa ggtataggca aatgtgttgg
     7021 cctagagaaa gaagactggg tatattcaac attgaatgca attacttgtg aaggacatgt
     7081 gtttaatgag tagtcaaaag caattagaag gcatgatttt cgatgtacaa agcttttcag
     7141 ttcatgatgg tccaggttgt agaacaactg tatttttaaa tggatgtcca ctgagttgta
     7201 aatggtgtgc aaatccagaa agttggactg taagaccaca tatgatgttt agtgaattat
     7261 cttgtcaata tgaaaatgga tgtactgtat gtcatggtaa gtgtaaaaat ggtgcattga
     7321 gctttaatct tgataataag ccagttatag attggaacat ctgtaaggac tgtgaaagtt
     7381 ttgaatgcgt caactcatgt tattataatg cgtttaaatt atgtgcaaaa ccttacactg
     7441 tagatgaact tgtgcaggta attaaacgtg attctaacaa ttggagaagt aatggaggag
     7501 taacatttag tggaggtgag ccactacttc agcatgaatt tttacatgaa gtattattaa
     7561 agtgtcatga ggtaaatata cacactgcca ttgaaacgag tgcatgtgta tcaaatgaag
     7621 tttttaataa aatatttaac gatattgatt ttgcatttat tgatataaag catatggata
     7681 gagaaaaaca caaagaacaa acaggtgtgt ataatgattt aatattagaa aatatatcaa
     7741 accttgcaaa ttctgattgg aatggaagat tagtacttag agtaccagtt atctctgggt
     7801 ttaatgatag tgatgaaaat ataagtgata ttatatcttt tatgcacaaa aataatttgg
     7861 tagaaattaa tctattacca tttcacagat taggagaatc aaaatggatt caattaggca
     7921 aggaatatga gtattctgat aagggtgatg ttgatgaagg tcatcttgga gaattacaag
     7981 atatattttt ggataatggt atagcttgtt atgttggtca tgagacagct ttctaaaatt
     8041 aaatacaagt tttaattaaa aaaggatgtt acactaaaag ctagagttac atcctttttt
     8101 atgtggtttt tatgtagaaa taattaagta aagttttata tattttatat attgtttata
     8161 ttgctttatg tccataaata ttgctttaaa tatactttat atatttttta tagtttaaat
     8221 tacagttaga gaatatatta cgtttacttt attgaagcag aagttttgtg gtaaaatact
     8281 actatgaaaa aatttaatag aatatatttt agaaacaact aaaaataata gttaggagga
     8341 agacatctat atgaacaaat catggagcaa cttgattatt aagaataaag acaaaaaagt
     8401 acgttcaggg tggattatta tagctgttat ggctaccttt tacatatttc agtattattt
     8461 aagcatgata cttgtggaaa taataagaaa aatcctggtt gctacagggg acattaattt
     8521 aaaaacaaac tatttttcag aatatgctaa ttggttaaat gatgttgggt tacagattgt
     8581 atttcaaatt ctaacagatt cacttatgat tattattcct ataattgtat ggagatttat
     8641 tatgaaaaat cctattagtg aaatgggatt acattcttat agaattaaga aaaaagaagg
     8701 atatgtagga atgttattag gaatatttaa ttgtacagta atctttatta taacaataac
     8761 aattggtggt ggtcatgtta cttcatgggt accaaaaatt tcacctttaa cagtttcttg
     8821 aatacttgtc tttattttag ttgcttttgc agaagaaatt ttaaatcgtg gtttttttat
     8881 ggcagtttta cgaagatgta aaaatattta ttttataatg attgttccat ctgttatttt
     8941 tgggttgatt catatatgga atcctgatgt tacttttttg tctgtaatta atattataat
     9001 tattgggatt cttttttcat atatgtttat aaagtcgtcg aatatatgga tgtgtatagg
     9061 ttatcatttt acatggaatg tatttcaggg gattatatat ggtatgcctg taagtggact
     9121 tcaggttcca gggattataa ctacacaaat cactcatggt aatctattaa atggtggtat
     9181 gtttggtatt gaaggtggaa ttttgactac ttttgttaat ctacttagtt ttatatttgt
     9241 atggtattac tatagagatt ctaagtatga ttttgttagt gataaagtaa atgtgaccaa
     9301 tatcaagaat tagttgatta taataaattt taataatatc ttctaagctt aaagtattct
     9361 gtaggattgt tggaaattta caaaaatata aaaataactt ggatattcta tttactccaa
     9421 gttattttta tattcttttg taaatagaaa tggaacataa aaaattaaaa caaccataga
     9481 tactattagt atatcatggt aagtgatagt tgattcttta gaatttgtga taagaaaagc
     9541 actattattt aaaatatgaa ttatagcaca aaaccagatg ttcttagttt ttatataaag
     9601 atatcctaaa aatattgaaa ttccaactat atgtatcgaa cgtaatatta atcctaatat
     9661 tggtgtttca ggtgtatata atgtaaatat tagtggtaag tgccacaaac tccaacaaat
     9721 acctaatatt ataactccta gttttttacc aaatttatta aaaaaaatat tctgtaaaaa
     9781 acctctccag gcatattctt cactaaaaaa aatataaata gtagttaatg atgttattgg
     9841 agtaaatata aattttatga taccactata attaggttga gacccttgta gtattaatat
     9901 aaatgatgtt ggaacaatta cagaaatcat aaagtaaata attaacaaag aacctagttt
     9961 aatatttttt ataggatata aatttttttt atttgatgaa tatattataa ttgataaact
    10021 acttataaaa gttaatatta ggtttataat tataaattca ttattattta ttagatttaa
    10081 taacttaaga acaattaata acgaataagt aagaaaaaat agtatgatta gtgaataaaa
    10141 ttcatacata tcattattta atccttcagt ataaaatttt gcaatagcaa ctgaagatag
    10201 aggtaatatc atcatgaatg atgcaaagtt ttctggatca ataaatttat tataatacaa
    10261 taataaacct aatgaataag ttaaaccaaa gtttataaag aaaaatataa taatttgtac
    10321 tttttttctt tttatttcat ttttaataag tattggattt agaatattta tccatattaa
    10381 ttttaagttc attaatttaa tactcc
//
LOCUS       DAMJVI010000120        10250 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.125, whole genome shotgun sequence.
ACCESSION   DAMJVI010000120 DAMJVI010000000
VERSION     DAMJVI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.125"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..942
                     /locus_tag="PC545_003160"
     CDS             <1..942
                     /locus_tag="PC545_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2457902.1"
                     /translation="CNYTKTFNSIYFVPLILDISFLIKEKYKYILFSFVIISSLIISL
                     DKNIYLALESSSILLIITVLSIYIENENLSKLYSQNLYDKLRISKDELKKVNADLETY
                     ASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLLKDKPLIYDLVHELREF
                     VTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNINVKLTISKNTWNLSRN
                     QSITLYRLIQESLSNSSRHGKATEIRIFITFNTSNLIITISDNGIGCGNIKKGNGLNS
                     INERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            935..1582
                     /locus_tag="PC545_003161"
     CDS             935..1582
                     /locus_tag="PC545_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457903.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKMNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTSEKTVKQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISQELFLSEGTIKNNITNILAKLELRDRTQLAIFAFKNKIVIE"
     gene            1711..2646
                     /locus_tag="PC545_003162"
     CDS             1711..2646
                     /locus_tag="PC545_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457904.1"
                     /translation="MDIVRVNNITKRFNDKLVLDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGKLLKERIIEALEVTGLTEVKKKKVKKLSGGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDISKKHETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NDYLKSLVSTNTKLIIEIKNINAQLIFDLKNTKGVISVLENNSLLELDIDKKLQLTDI
                     LSVIDKNNSKIMKISYEEPSLEDVFLNLTGKNLRD"
     gene            2660..3757
                     /locus_tag="PC545_003163"
     CDS             2660..3757
                     /locus_tag="PC545_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457905.1"
                     /translation="MKLYMSVKTMLKSLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNDLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIINNNVKQNSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRILGITFKGNFAILLLLCALSSYFMTSISTFVTS
                     FFSKKYGTAIVYALLFLQTIFGGIFSMISDTFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGDLIFTCLIISTILIALAIVKEKYKWREV"
     gene            3760..4824
                     /locus_tag="PC545_003164"
     CDS             3760..4824
                     /locus_tag="PC545_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457906.1"
                     /translation="MIRLTIANIKRYLKNHNLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNELDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDKLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKVDKNIDDKLTKTNIIDN
                     KPDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFALLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWVARVFKNESSIS
                     LISTFYSLIGLAISISSLIPSMDKLSFMTNLSKFTPFYWTIDAIKNNGSILINIIALI
                     LIGIIFVTAGSLKLRDFAKN"
     gene            5068..5565
                     /locus_tag="PC545_003165"
     CDS             5068..5565
                     /locus_tag="PC545_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HDF2457907.1"
                     /translation="MYKIGESVMYPKEGACSVNDIVTKKINHEMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDNFIQSINKTDGVWIFDRKERLKLYQDKFHSGDVF
                     EIVKLIKMLMIQDSSKQLCSTDKEFLNKAQKFALSELAAAQCKSYTMVLEEMKKHILN
                     SKNTN"
     gene            complement(5741..5989)
                     /locus_tag="PC545_003166"
     CDS             complement(5741..5989)
                     /locus_tag="PC545_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457908.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(6055..7071)
                     /locus_tag="PC545_003167"
     CDS             complement(6055..7071)
                     /locus_tag="PC545_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2457909.1"
                     /translation="MEYNELLKSARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVKQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            complement(7129..7896)
                     /locus_tag="PC545_003168"
     CDS             complement(7129..7896)
                     /locus_tag="PC545_003168"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDF2457910.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYDKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDISQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            complement(7964..10153)
                     /locus_tag="PC545_003169"
     CDS             complement(7964..10153)
                     /locus_tag="PC545_003169"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HDF2457911.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRELAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGIGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEDFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNFMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKEDKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
BASE COUNT         3565 a         1569 c         1187 g         3929 t
ORIGIN      
        1 tgtaattata caaaaacttt taattcgatt tactttgttc ctttaatatt agatatatca
       61 tttttaataa aggaaaaata taagtacata ttgttctctt ttgttataat atcttctctt
      121 attattagtt tagataaaaa tatatatttg gcactggagt caagctctat attgctaatt
      181 ataactgtac tttctatata tatagaaaat gagaatttat caaaattata cagtcaaaat
      241 ctttatgata agcttagaat ttctaaagat gaattaaaaa aagttaatgc tgatttggaa
      301 acttatgcaa gttctataga agaattagca attttaaaag aaagaaatcg aatatcaaga
      361 gaaatacatg atagtgtagg acattctctt tctacaacaa taatacaatt aaatgctata
      421 gaaaaactct taaaagacaa acctctaatt tatgacttag tacatgaact tagagaattt
      481 gtaactgaaa gttttcaaga tgtaagaagg gctatatctg agttaaaacc tgttgaatat
      541 gaaaattacc aaagtttatt taaaatcaaa gaattagtta aaagctttat taagcttaca
      601 aatattaatg ttaaattaac tatttcaaaa aatacttgga acttatccag aaatcaatct
      661 ataactttat atagacttat acaggaatcc ctttccaact catctaggca tggaaaggca
      721 actgaaatca gaatttttat tacattcaat acttcaaatc ttataattac tattagtgat
      781 aatggtatag gatgtggcaa tataaaaaaa ggaaatggac ttaatagtat caatgagaga
      841 atatatgaat taaatggtaa agttgaattt gataattcta ataatggttt cacaattaga
      901 gcttcttttc ctaaattttc agggggtgat ttttttgaat aaaattaaag ttcttatagt
      961 agatgacgaa aaattaatta gaaaaggtct taaaattata ttatcatctt ataatgattt
     1021 agaaattgta ggagatgctt ctaatggata tgaagcttta gaattttgta aaatgaatga
     1081 tgtagacata gttcttatgg atatacgaat gaaagtatgt gatggggttc taggtacaag
     1141 gcttataaag gaatataata attctataac attattaatt ttaacaactt ttaatgatga
     1201 tgaatatata aaagatgcta tgaagtttgg tgcttcagga taccttttaa aagatagctc
     1261 tgatgaagta ttgcatgaag ggattcgttc ttcatttttt ggcaatattg tgcttgataa
     1321 aagtgtagct gaaaaaatca tgacctctga aaaaacggtt aaacaagagc atctttatga
     1381 catgtacaat ttaacagaaa aagaaatatc tataattaga ctaattgcaa atggacttaa
     1441 taataaagaa ataagtcaag aactattctt atctgaaggt acaataaaga ataatataac
     1501 taatatttta gctaaactag aacttcgtga cagaactcaa ctagcaatct ttgctttcaa
     1561 aaataaaatt gtaatagaat aaatctaaaa atataaagtg acttaagtca ctttttttag
     1621 tgacttatag taatagaatt ataattcaca attctttatt ataatattat agctttttaa
     1681 cttatacaaa attattttgg agggattact atggatatag tcagagtaaa taatataaca
     1741 aaaagattta atgataaatt agtacttgat aacatcagct tttctgttaa aaaaggtgag
     1801 atatttggac ttattggccc aaatggtgca ggtaaatcta cacttataaa tataattaca
     1861 aatcttatgt taccaaatag tggtagtatt caaatcaatg gtttagattt atcaaaagat
     1921 tatataaaag caaagtctat aatagggtta gttccacaag aattagcaat tatagaaact
     1981 cttactcctt ttgataactt agagtacttt ggagctttct acggtttaaa gggaaaacta
     2041 cttaaagaaa gaatcattga agcattagaa gttacaggtc ttactgaagt caaaaagaaa
     2101 aaggtcaaaa aattatctgg tggtatgcaa aggagattaa atattggaat agcactctta
     2161 aatcatccta aaatattaat attggatgaa ccaactgtag gtgtagaccc ccaatcaaga
     2221 aatcatattt tcaattttat aaaagatata tctaaaaaac atgaaactac tgtaatatat
     2281 acttcccatt atatggaaga agttgaacac ctttgttcta aaatttttat tatggatgaa
     2341 ggtaaagaaa ttgcttttgg agataatgat tatttaaaat cactcgtatc tacaaataca
     2401 aaattaatta tagaaataaa aaatataaat gcccaactta tctttgattt aaaaaatact
     2461 aaaggtgtta tatctgtttt agaaaataat tcattattag aattggatat agataaaaaa
     2521 ttacaactta cagatatact ctctgttatt gacaaaaaca attcaaaaat aatgaaaatt
     2581 tcttatgaag aacctagcct agaagacgta tttttaaatc taacaggaaa aaatttaagg
     2641 gactaatgga ggtgatttta tgaaattata catgagtgta aaaactatgc taaagagttt
     2701 aaaaagctcc tttatactta atttaatata ttttttagct ttgcctctga ttttatcttg
     2761 gtttcttggg atggttacag aaagcatgtt tcaaaaccct ataaaaacag aatctacccc
     2821 cattgttata tatgacaaag ataacacaag attatcaaat gatttgacaa agtatttgaa
     2881 aaatgattta tcttatatat taacagttaa aaaagatgat tctaaggcag aattaaagct
     2941 tactatacct aaaggctatg agagtagttt attaaatgaa aaatcaaata ctctaaacat
     3001 agaaaaactt ggaactcgtg atgatattgc aatattgctt caagatatat tagatactta
     3061 ccatgaaaaa ttttatttaa ataattctca gaaaataagt tcagaagatt tttctaaatt
     3121 atttaataaa aattctattg atacttctat aataaataac aatgttaaac agaactctta
     3181 tgaatacttt gctttagttt cactaggatt tcttgtaata atttttataa tgaacaatat
     3241 tttgtcaaat tatataagtg aatcaaaggg actttcaaaa agactttatt ctatgcctat
     3301 aactagagta caattcttaa tttatgactt tgtaggttta tggatttatt catttatatt
     3361 cttattatta tatgttttat tctttagaat acttggaata acatttaaag gtaattttgc
     3421 catattgctc ctactttgtg ctttatcatc ttattttatg acttcaatat ctacctttgt
     3481 aaccagcttt tttagtaaaa aatacggtac agcaatagtt tatgcattac tctttttaca
     3541 gacaattttt ggaggtatct tcagtatgat aagtgataca tttactaagc ttacaagttt
     3601 atctcctacc tatttaatag gagaattgtt tagtaattat gaaacattta aaactataga
     3661 ttcaatagga gatttaattt tcacatgttt aattatctct acaatattaa ttgcacttgc
     3721 tattgtaaag gaaaaatata aatggaggga ggtttaataa tgattagatt aactattgca
     3781 aatataaaaa gatatctaaa aaatcataat cttttaatta atatggttat gcttcctatt
     3841 atactaattt tttcacttaa tttttttatc aacaactcag gaaatcaatc aatgtacttt
     3901 tctccagtag caattgtatc tgactcttca ggaaagtatg aacataagtt aataaatagt
     3961 tctaaattaa aagaaaattc atttagatta aatgaactag ataaggctat gaatctatta
     4021 aaaaataata aggtttctgc tgtattcgtt ttagataaaa atttttcaaa tagtatagat
     4081 aaattacaac gacctgtagt taaatgcttt aaaattgaaa atggtggtgg tagtctttgg
     4141 gcagaatcac aaatagaatc ttttattact aaatcgttaa aattgaaagt ggataaaaat
     4201 atagatgata aattgacaaa gacaaatata attgataaca aaccagataa gaataaaggt
     4261 tcatttttag tagtcttctt gatatgctat tttatgtata ttaatgcagc acatcttgct
     4321 tctgatttat ttacattaaa aaaatcaaat gtgctaaaaa gacttatctc tacagataat
     4381 aaagatataa aaataatttt cagtatattt ttaggactat tttttattca atcaattgta
     4441 tacacttttg ctctattatg tttttcaatt atagaagact ttaatttgag tctgaatgta
     4501 ttgatgattg tactagcaaa tagttttgtt tccactggat ttgtattttg ggtagctaga
     4561 gtatttaaaa atgaaagttc tataagtctt atatctacat tttattcttt aataggtcta
     4621 gctatatcta tctctagtct aataccatca atggataaat tatcctttat gactaatctt
     4681 tcaaagttca caccatttta ttggactata gatgctatta aaaacaatgg tagtatttta
     4741 ataaatataa tagctctaat attaattgga attatatttg taactgcagg aagtcttaaa
     4801 ctaagagatt ttgctaaaaa ctaataatta ttaaatataa ttagtatcta tttttatata
     4861 gttgtaaaag aatatttcaa catattaaat tattttatct tatatttttc tctttagttt
     4921 cacacatcac caagtctaaa ccttatatta tatattaata tttttgactt gtcaagttat
     4981 ttatgttata atattaatca ttagataatt aaagcattgt acctaagttg caatatctaa
     5041 aattcaattt ggaggtataa ctagcatatg tataaaattg gtgaaagtgt aatgtatcca
     5101 aaagagggag cctgctctgt aaatgatatt gtaacaaaga aaataaatca tgagatgcaa
     5161 aaatactatg aattaagtgt catttttaat agtaacctta aaatttcgat tccagttcta
     5221 aatgcagata gaattggcat acgtccagtt atggatggaa atgatgttga taattttatc
     5281 caatctatta ataaaacaga tggtgtatgg atttttgata gaaaagaaag acttaaactg
     5341 taccaagata aatttcattc aggagatgtt tttgaaattg taaaattaat caaaatgctt
     5401 atgatacaag acagttcaaa acaattgtgt agcacagata aggagttttt aaataaagct
     5461 caaaaatttg ctttatcaga attagctgct gctcaatgta aatcctatac aatggtatta
     5521 gaggaaatga aaaaacatat attaaattca aagaatacaa attaaaccag ttttttaaac
     5581 tggttttatt ttttatacta atcatccatg ctactttagc ttattttaaa ctgcctaatc
     5641 taatattaat aaagttatct caattagata gatgtatttt attcaaataa aaaaaactaa
     5701 gagtaaatca tattactgat ttactcttag ctaataaaat ttaatcttct tcaagttctt
     5761 ttttctcttg taataataca cttaattcat ttgcttctaa aacttgtgcc tctgcaatat
     5821 caagtaactc ttgttgccat tccacacatt tatctttgta atcaaaaggt agcgttctgt
     5881 caactatgta cttatcaata cagtcctccg cacttttaat tatttctttt ctttcatcaa
     5941 taaatttttc taattctact aaggcttctt cataagcttt tctactcata taaattcccc
     6001 ctttgtacaa ttattctaca ttaaaatgat ttttcctctt ttttgtataa aattttattt
     6061 gtatataact tttccatcaa tatcttttat agtttgacat ccagttaata tcatagaact
     6121 acttagctct gattgaagtt tatttacata agtttctaca ccatcagttg caccaccaaa
     6181 agaagcagtc acaaaaggtc ttcctattaa tactgcatct gcacctaaac ctatcatttt
     6241 tagtacgtct aaaccagttc tcactccacc atctgcaagt attgtaactt tacctttaac
     6301 agcatcagca atttttggta aaacttcaca tgctcctggt gtacagtcaa gcactcttcc
     6361 tccatgattt gatacaacta ttgcatccac acctgcttct actgccatta aagcatcttc
     6421 aacagtcatt atacctttca atataaatgg tagtttagta gactttacta actcctttat
     6481 ttgtttaaca tttttaggaa gtactggttt tccatgtaat gataaagtta ctagaccaca
     6541 ggcatctata tcaactccaa ctgcaaatgc accagcttct tcagctaatc ttatcttttt
     6601 tataatattt tcattatccc aaggtttaat aaatacaatt ccagctccat tatacttttt
     6661 gactacatct aaattttcca ttaaaaaagc atctactgcc gtatcaccaa ccattgcata
     6721 aataccacta ttagcacatc ctgcaactac tggttctatg taatctcttt cattaatttt
     6781 tccacccata tttaaagtag ttcctgttac tggagctgca aatataggtg cactcatttt
     6841 ctttccaaac atctctatag atgtatcagg atttgataca tcatgtatta ctctcatatt
     6901 tacttttact ttttctaaac tcttgctatt ttctataaaa gaagaaccac ttcctttacc
     6961 tcccatgcca ggcacttcac ctgcacaagc aaccccatta caaaccttac aaactttaca
     7021 acttccattt aaattttctc tagcactttt taataattca ttatattcca tctttaatcc
     7081 cccattaact atagtaattt atattatata cattatatca tattatattt acagtatatt
     7141 tttcaaaaaa ctttttctgt ggataggagt tatgccatat ttttcaatag cttcatagtg
     7201 ttctttagtt ccatatcctt tgtgagattt aaacccatat tctgggtata ctctatcata
     7261 ttggtacatt atactatccc tagtaacttt agcaagtata cttgctgctg ctattgatat
     7321 agattttgaa tctcctttca caattggatt ttgagatatg tcaatacctg gtattgttgc
     7381 tgcatcaact aataaataat ccggtgcttt ttttaaacaa tttatagcct ttttcattgc
     7441 catatatgta gcatttaata tattaaattc atctatttct tcattgttta ctatacctat
     7501 tccataatct agtgcttctt cttttattac ttcaaaaagt tcatctcttt ttgcctcact
     7561 cagtttctta gagtcattta caccttctat tttggtatct tttttaaata ctacaacaga
     7621 agctacaaca ggtccagcaa gtggtcctct tcccgcctca tcaattcctc caatatataa
     7681 ataaccttta tcatagcctt cgttctcaaa tatatttata gtttccaatc tttcttcttc
     7741 ttttctaata ttatccagct tttttgctag ttttatagct agaccttgta cagattttct
     7801 ttcatcaact cttaacagct ctatatattc catatatttt tctacttcta aagtctctat
     7861 tatttcttta atctccctca cacttttatc ctgcatacca ttccctcatc tttcgttttt
     7921 aaaaatttaa tttttaaata tattaaaatt ttttaattag agtttaattg catccactat
     7981 agccacattt taaacatata ctacatccac ctgtatgtgc aattccttca cttccacact
     8041 caggacacac aaatttttca tcttcttcaa aatttttatt tattatttct ttggcttttt
     8101 catctttatt atctttttct tttttattat ttttataata ttctttttta ctattttctt
     8161 ttttatcttc tttattattt tcttcctttt tttcatttat taaaattcca ctccttttac
     8221 atccatctct aaatattgtt actcctttaa gaccagcctc ccatgcctta atatatatat
     8281 tgaaaacatc ttcaactgta gtttcaaaag gtatatttat tgtagatgat atagaagcat
     8341 caatatactt ttgccatact ttttgcatgt ttattctttc ttcatatcct atattcatag
     8401 cagtaacaaa aaaatctggt aaacattctt catctgtaat acctttcttg tccatatact
     8461 ctttaacaat tggagtatat actttataat acacatcctc atcatgcaag ctttcagttt
     8521 ttcttatata tgaaagatta aatataggtt ctattccacc actaatacct atcattgtag
     8581 atatagaacc tgtaggaggt atggttaaaa gttgtgaatt tctaagccca tactttttaa
     8641 ccatttcctt agtgtcttca tttagatttt ctataaaaaa cttagattta gaaatatttt
     8701 catatttata ctcactaaat gttccatatt ctttagcaat tagtgctgat tgcttcacag
     8761 cttcatttaa catagtctta ccaattaaat cacatagttc taaagattca tcagaaccat
     8821 atcttattcc catttttata agcatatcag ctatacccat tacacctaaa cctatctgtc
     8881 tatacttatg tacactatct ctttgttctt gtaatggatg caagttcaat ccctcatcta
     8941 agacatcatt taaagctact acagcttctc taacgcaaga ttttaattta ttaatatcaa
     9001 aagtagcatg ttcaccaaat ggttgtatta taaactctga caagtttatt gagccaagta
     9061 gacaacttcc tccacttgga agtggttctt ctgcacaagg atttactcct gaaaagcaaa
     9121 agttcttatc ttcactcata aaattatatg tttttatttt atcccaaaac aatatacctg
     9181 gttctgcata atcccagtta tttttcacaa atttattaaa taaatcataa gcatttacta
     9241 tcttaactat tttttctcca gtagtttcaa cttcaaaagt acaattatat aacttttttt
     9301 ctcttacagc ttgcataaat tcatctgtta ctcttacaga aatatttgct ttagtaacct
     9361 tctccaaatc tgtttttata tctataaagt cttctatctc tggatgattt acatccatag
     9421 aaatcataag agctcctctt ctccctcttt gaccaattaa gccagttgtc atactgtaaa
     9481 gttccataaa agagactgct ccagtcgtat gttttgcagc attgttaact ttcgctcctt
     9541 ttggtcttag ctttgaaata tcaattccaa ttcctccacc ataactaaaa gttctagcaa
     9601 gtttttttgc agtatcaaat atatcttcta tgctatcttt tggtggttct attacataac
     9661 aattagaata tgttactttt tttccttctt tctgtaatcc tctatttgct aaaattcttc
     9721 cagcaaatag aaatcttttt tgtcttatta gttttgatat tcttgaacta ttatttgaaa
     9781 ttctctttat ccattcatca aaactctcac cattatgtct atacttgttc ttccatattg
     9841 atttttgtaa ttcatcaaac tcccaagctt tagtccttat ttcagagcgt ctttctctat
     9901 ataatatgta gtgttttgct atatcttttc tatttgattc cataagtagt acttcaacta
     9961 tatcttgtat ttcttctacg gataagcatt tttccataga attatagtca ttagctattt
    10021 ttcttgatat atcatatgcc aactctctat ctactccacc ttcactattt atactagctt
    10081 tatagacagc atcttcaatc ttacttttac taaaaagttc ttttgtacca tctcttttta
    10141 ttatgtattt catttttaaa cctccaataa attagtatta aattaataca cacattaact
    10201 ataactctaa acaaactttt tatatatttg tatattttca tattaattat
//
LOCUS       DAMJVI010000121        10246 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.126, whole genome shotgun sequence.
ACCESSION   DAMJVI010000121 DAMJVI010000000
VERSION     DAMJVI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.126"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..249
                     /locus_tag="PC545_003170"
     CDS             <1..249
                     /locus_tag="PC545_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2457912.1"
                     /translation="EEDEFVQAAYKGLKEAGIDSEITQYSFCTNGSHYAGEAGIRTIG
                     FGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSVYKK"
     gene            265..1848
                     /locus_tag="PC545_003171"
     CDS             265..1848
                     /locus_tag="PC545_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HDF2457913.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDYNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDTIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIKDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            1876..3204
                     /gene="ssnA"
                     /locus_tag="PC545_003172"
     CDS             1876..3204
                     /gene="ssnA"
                     /locus_tag="PC545_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDF2457914.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTEEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISAEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            3349..4701
                     /locus_tag="PC545_003173"
     CDS             3349..4701
                     /locus_tag="PC545_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDF2457915.1"
                     /translation="MSRDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLKFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVLLNIFFNELGSKKEKNNKVEEAK
                     "
     gene            4893..6002
                     /locus_tag="PC545_003174"
     CDS             4893..6002
                     /locus_tag="PC545_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2457916.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKETGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMAARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            6285..7676
                     /gene="hydA"
                     /locus_tag="PC545_003175"
     CDS             6285..7676
                     /gene="hydA"
                     /locus_tag="PC545_003175"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HDF2457917.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGVTKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     DGLEEWHEKAKDKSYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKSENNLSPKYHQLTRPAIMEAE
                     ATSRLMKIAEVANSKVYVVHLSCKESLEEVLKAKQRGVDVIVETCPQYLLLNDELYDL
                     DGFESAKYVMSPPLRNKENNEILWNAILDNQIDTIGTDHCSFNYKGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGTIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            7670..10231
                     /gene="xdh"
                     /locus_tag="PC545_003176"
     CDS             7670..10231
                     /gene="xdh"
                     /locus_tag="PC545_003176"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HDF2457918.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTEDALREDAPKIHETGNILVKEHLVRGNAKEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEEDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAMAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     MTQVVCETASLLPENVVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASNKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVILDENGKLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVNSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
BASE COUNT         3964 a         1236 c         2137 g         2909 t
ORIGIN      
        1 gaagaagatg aatttgttca agcagcgtat aagggattaa aagaagctgg aatagattca
       61 gaaataactc aatactcatt ctgtacgaat ggaagtcact acgcaggtga ggctggaata
      121 agaactatag gatttggtcc ttctaaggaa aatcttgctc atacaataga tgagtatata
      181 gaattagacc aattattcaa aggtgcagaa ggatattatg gtatattaaa atcagtttat
      241 aaaaaataat taggagatgg ttttgtgaga actttaataa aacatgggct tatagtagat
      301 ggaaataaaa caccagcata tgaaggagat attttaatag aaaacgagaa aatattaaaa
      361 atatcacaag atttaaacga agaagctgac aaagttatag atgcaaaagg tagagttata
      421 tgtccaggtt tcatagatac tcatagccat tcagacctag ttattttagt taatccatat
      481 aatgaggtaa agattagaca gggaattaca acagaagtgt tgggccaaga tggtatatct
      541 atggcccctc tacctcaaga gcatatatct tcttggagaa aaaatttagc aggtctagat
      601 ggtgaaagtg atgaaattga ttggaaatat gaaacaacag agaattattt aaaaatgatg
      661 gattacaatg gggtaggttt aaatgaaact tatttagtac ctcatggaaa cgttagaatg
      721 gaagctatgg gacttgaaga tagacctgct actaaagaag aaattcaaaa aatgtgtgaa
      781 atcacagaga gagaattaaa agcaggagct ataggattgt ctactggact tatatatata
      841 ccttgtgcat actcattgac tgaagaaatt atagagatgt gtaaggtagt agcaaaatat
      901 gacggagtat ttgtagttca ccaaagaagt gaagcagata caattttaac ttcaatggaa
      961 gaaatcatag aaattggaaa acaatcagga gtaaaagttc atttttcaca ttttaaggtt
     1021 tgtggtaaag caaactggaa gtatatacca caagttatag agcttttaga aaaagcagaa
     1081 aaggaaggaa taagagtatc ttttgaccaa tatccatatg cagcaggaag tacaatgctt
     1141 ggagtagttt tacctccttg ggcacattct ggaggaactg ataagttaat agaaagatta
     1201 agtgatgaaa acgaaagagc aaaaatgaaa aaagatatag caaatggaat agaaggctgg
     1261 gataatttta tagaatttgc tggaatagac caaatatttg taacaagcgt aaaaacagaa
     1321 aagaataaag atacaattgg taagagctta ttagaaattg gaaaaatgag aggaaaagac
     1381 ccattagatg caacgtttga tttattaaaa gaagaagaaa atgcagttgg gatggtagat
     1441 ttctatggat tagaagaaca tataatagga ttcatgaaaa gagacgagca aaatgtgtgt
     1501 acagatggtc ttttagcagg taaaccacat ccaagagctt atggttcttt ccctaaaata
     1561 ctaggaagat atgttagaga gttgaatgtg ttaacaatcg aagaagctgt ttataaaatg
     1621 actaaaaagg cagctacaag ctttagtata aaagatagag gcgaattaaa agaaggatat
     1681 tttgcagata tagttatatt tgacaaggat acagttagtg gttgtgatga ttttatcaac
     1741 tctatgcagt accctacagg tatagattac gtaattataa atggaaattg tgttgttgaa
     1801 gaaggaaaat acaataaaat taaagcagga aaagtactta aaaattaaat cttagcttta
     1861 aggaggaata aatttatgat tttagtgggg aatgggaggc taataagcca agattcttta
     1921 aatccctata tggaagatgg atgtgtagta ataaatgata atctaattga agatataggt
     1981 actacagaag aaatgaaagc taaatatcca gtacatgaat ttatagatgc aggcaagaaa
     2041 ataatcatgc cgggtctaat aaattcacat atgcatatat acagctcatt tgctagagga
     2101 atggcagttc caggaaaacc ttctgagaac tttttagaga tactaaataa tttatggtgg
     2161 agacttgaca aacagcttac actggaagat acaaaataca gtgcatatgc aacgtatata
     2221 gaatgtataa aaaatggtgt aacaacagtt tatgaccatc atgcaagtcc aaatgcaatt
     2281 gaaggcagtt tgtttacaat atcagatgtt gcaaaggatt tgggaattag aacgtgtctt
     2341 tgttatgaag tgtctgatag agatggtgga aatacaatcc aacaaggtat caatgaaaac
     2401 gtaaacttta taaaatacgc tcaaaaagat gatacagaca tgataaaagg aatgtttggg
     2461 cttcatgctg catttacact atctgatgat acattaaata aatgtgcttc agagatggct
     2521 ggtcttgata caggttacca tgtacatgtt gctgaaggac tagcagactt acaatataat
     2581 gtagataaat atggaaaaag ggttttagaa agattcaatg attttggagt actaaaagaa
     2641 aaaacaatag cagttcattg tatatatata agtgcagaag aaatcagtct tttaaataaa
     2701 acaaatacat ttgtagttca taatccagaa tctaatatgg gaaatgcagt tggatgttct
     2761 cctgcaatag agttattaaa aaggggagtt acagtaggat taggaactga tggatatact
     2821 agcgatatgt ttgaatcatt aaaagttgcc aatataattc acaaacatca cttaaaagac
     2881 ccaagagttg gatttgaaga atcacaaaca atgttatttg ataacaatag aaaaatagct
     2941 aaagcttatt ataaaaatga cctaggagta cttaaaaaag gtgcttatgc agatgttata
     3001 gtagttgatt atataccaca tactccattg acagaaaata caataggggg acacactatt
     3061 tttggaatga gtggaagaag cgtagataca actattataa atggaaaggt acttatgaaa
     3121 gatagaaagt taataaacat agatgaagaa agtattttag caaaaagtag agaattaagt
     3181 caaaatctat ggaatagaat ttaaataatt gatattaata tacaagtaag aaacaagaat
     3241 ttagatgtta aaaacagagc ttaaacaaaa ataggaattt aaatgaagta tataaataaa
     3301 aaatatacgg aatagaatat taaattaaat atagaaggga gacccaaaat gtctcgagat
     3361 aaaagcccta aaatagctcc agtagatgaa aggttacctg tatccaaatc ttgggtattt
     3421 tcactacagc acgttttagc aatgtgtgca ggagcagtag cagttcctat gatgatagga
     3481 agtgctgcac gactagatca tgctagtatt gtttttttaa taaatgcttg cttgtttatg
     3541 gcaggtatag gaacactcat acaaagtgtt ggtataggaa atattgtagg tgctaaaata
     3601 ccagtaatag aaggaactag ttttgccgct gtggcaggta tgacagcaat agcctcatca
     3661 tatagtgatt cacaagttgc aatacagaca gtatttggct ctgtaatagt agctggattg
     3721 ttttgtatat tacttgctcc agtatttgaa aaattaataa gatttttccc tagtgtggtt
     3781 actggaacag ttgttatggt aataggtatt tcactatttc cagttgggat aaaatggata
     3841 actgacaaca aattggaagc ggttgaacca aactcattag cattagcttt agcagtattg
     3901 gcaataactt tactttcatt taaattctta aaaggaattt ggaatagtac agctatattg
     3961 gtaggtatag tacttggtac actactttcc atggcagttg gaatggcaga ttttagtgca
     4021 gtaaatggag ttggttggct taacttaaat gtacctttaa aatttggaat gcctagattt
     4081 gaaccatctg caatactatc attgtgtttg attatgttgg tacttatgac ggaatcagtt
     4141 ggtaatatga tagctattca tgaaatggta gacaaagaag taacaggtaa aaatattaaa
     4201 agaggtctat ttggagatgg aatatctaca gcattatctg gaatattcaa ttcatttcca
     4261 ataactccat ttggtcaaaa tacaggaata gtaggtctta caggtataaa atcaagattt
     4321 atagcagttt acgcaggggt tatattgcta gtacttggat tcttaccaaa atttgcagca
     4381 ataataggag caatacctaa gcctgttctt ggaggcgtag gatttgcaat gtttggaatg
     4441 gtaatggttg gaggagtaaa aactttaagc aaagtgaaat ttgatggaac taaaaatggg
     4501 gttatagtag cagttagtgt tggtttgtct atgataccac taacaaatcc tgatttctat
     4561 cataatttcc caacttgggt acaaacaata ttccatagtg gaataacaac aggtagttta
     4621 tcagcagtgc ttttaaacat attcttcaat gaactaggtt ctaaaaaaga aaaaaataat
     4681 aaagttgaag aagcaaaata aaaataaaac aagtaaaaat ctatattgac ttaaagtcat
     4741 taagcattaa taaaaataat tcagagatat taataattca acaaaactat cttatatttt
     4801 aaataatata aaaaagtaaa taaataacga atacaaataa aagaatacaa tctaaatatt
     4861 aaaaatattt aatttaaggg agagataata atatggctaa tttaaaagtt aatttatatg
     4921 gaatggaatt tttaaaccca attatgacag cggcaggacc tggagcaaaa gatggtgctc
     4981 tctgtcaaga ggcagcaaaa ggtggagcag gagctatatg tacaaaaact atatctgtat
     5041 taccagctga tgtaccaaga ccatgtatgg caaaaactaa tagtggtttt ttaaacacag
     5101 aattatggtc agagcttcca aaagagcaat ggatagagga agaatacaaa aaggtaaaag
     5161 aaacagggct tccagtaata gtaagtatgg gatattcagc agagcaaata gctgaggttg
     5221 ctcctttagt aaagccttat gcagatgcag ttgaattatc tactcattat gtaggtacag
     5281 acgttgcacc aatagtaaat gctttaaaag ctgcaaaagc tgttttagat gtaccagtat
     5341 ttatgaaaat gagtcctcat actgacattc aaactattgc aaaagctgtt gaagaagcag
     5401 gtgcagatgg acttgtaatg ataaactcat ttggaccatg tatggcaata gatgtaaata
     5461 caggataccc tataatggga agtaaagctg gatacggatg gttatcaggt gcaccaataa
     5521 gacctctagc aataagatgt atatatgaag cttctcaagt tgtaaatata ccaataatcg
     5581 gtgttggtgg aatcacttgt ggaaaagacg tggctgaaat gatgatggct ggagcaagtg
     5641 ctgttcaagt atgtacagaa gcaatcctta aagggccttc tatctatgga aaaattgcta
     5701 aagaattaaa tgaattttta gatgagaatg gatatgctga tgttaatgaa ataaaaggat
     5761 taactcataa gaaaatggca gctagagggt ttagaactca ttcagtagct ccagtagtgg
     5821 atgataacaa gtgtatcaaa tgtaagcaat gtgtaacaag ctgtgtatac gaagctttag
     5881 aagttacaga taagttgaac atagatgcag acaagtgttt tggatgtgga ttatgtgtaa
     5941 ctagatgtcc aaaaggagct ttaagcatct gctacaatca agtagaagct atgaatgagt
     6001 aataattata tagaattttg gtaattaact tagagtaata ggtattagga taataaaata
     6061 tatcagataa acaaacacgc attatgcatt aataatacag tctaaagtta ataattttat
     6121 tatattgatt taactaaata ttagtttagc tgaatattaa tttaattagg tattggttta
     6181 atcttaatat agatttacta cgtataagtg aactaactag attactttaa gttaattttt
     6241 tcatataaaa taaattgata cattacaagt aaggtggagt ctatatggga attgttttaa
     6301 aaggtgggaa agtagtaaca agtgaaaaaa cttactgtgc agatgtaaga atagaagatg
     6361 aaaaaatagt cgatataggt cttgatatat caaaagaaaa tgatgaaata gttgatgtta
     6421 gtgattctta tgttatacca ggaggtatag atacacacac acactttgac ttagatacag
     6481 gcgttactaa gacagcagat aactttgaga caggaacaaa agctgctata gtaggaggaa
     6541 ctactactat attggatttt gctacacaag ctaaaggatg ttctttgaaa gacggattag
     6601 aagaatggca tgaaaaagct aaggataaat catattcaga ttatggcttt catatggcta
     6661 taacagattg gaatgactcg gtttgtaatg aaatggagga tatggtgaaa gagggagtat
     6721 cttcatttaa actgtatatg gcgtataaag gaagccttca agttgatgat ggtgtaatct
     6781 ttgaagcatt gagaaaagca gaagagatag gagggattat tggtttccat tgtgaaaatg
     6841 gggacataat atgtgaactt gtggataaag caaagtcaga aaataattta tctccaaaat
     6901 atcatcaatt aactagacca gctataatgg aggcagaagc tacatcaaga cttatgaaga
     6961 tagctgaagt tgcaaactct aaagtatatg tagttcactt aagctgtaaa gaatccttag
     7021 aggaggtttt aaaagctaag caaagaggtg tggatgtaat agttgaaact tgtcctcaat
     7081 accttctatt aaatgatgaa ttatatgatt tagatggatt tgaaagtgct aagtatgtaa
     7141 tgtcaccacc tttaagaaat aaagaaaata atgaaatatt atggaatgct attttagata
     7201 atcaaataga cactatagga acagaccatt gttcttttaa ttataaggga cagaaggaac
     7261 ttgggataaa cgatttcagt aagataccaa atggttctcc aggtgtagaa cataggatga
     7321 gtcttttata cacatatgga gtctgtaaaa ataaaatatc tatcaacaag atggtagaag
     7381 taacatcaac taatgcagca aagttgtttg gaatgtatcc taataaagga actatagaaa
     7441 taggcagtga tgcagatata ttagtcttaa atccaaacaa aaaatccaaa atagaagcaa
     7501 aaaaacaatt acaaaatgta gattatacac cttatgaagg gtatgaagtg gattgtcaaa
     7561 tagaacatgt ttatttaaga ggaaaagaag tagttagaga tggagaatta atttgtaata
     7621 ctccaagtgg gagatatata aataggcgtc taacaaaggg ggctagagaa tggtagaatt
     7681 tatattaaat ggagagaaag ttgttgcaaa agaaggcaaa aaacttttag acttccttag
     7741 agaagatatg aatataactt cagttaaaaa tggatgttca gaaggtgctt gtggaacatg
     7801 tatggttata atagatggta aagctactaa agcatgtgta tttaaaactg ataaattagc
     7861 tgggaaaaac ataacaacta tagagggatt atcagagaga gaaagagatg tttttgcata
     7921 ttcttttgct agtcaaggag ctgtacaatg tggattttgt attccaggta tggtaattag
     7981 ttcaaaagca cttatagacg ttaataataa tcctactgaa gatgatataa agaaagcttt
     8041 aaaaggtaat atatgtagat gtacagggta tgtaaaaata attaaagctg tcgatttagc
     8101 agcaaagatt tttagagaaa atatagatgt gccaaaagta aactgtaaag gtctagtagg
     8161 tgaaaatctt cctagagttg atgcagttgg caaagcactt ggatattctg aatatgtaga
     8221 tgatatgaga attgaaggta tgatttatgg ttcagcagtt agagctaagt atcctagagc
     8281 tttagttaaa aaaatagata tagaagaagc aaaagcttat ccaggagttg ttgcagttct
     8341 aacagctaaa gatattccag gaactataaa ggtagggcac ttaaagaagg attgggatac
     8401 tttaatacca gaaggtgaaa taactagata cttaggagat ggaatagttt tagtagcagc
     8461 agaaactaga gaagcgttag aagaggcaaa aaagctagtt aagatagatt atgaggaatt
     8521 aacaccatta agtactacag aagatgcgct tagagaagat gctccaaaga tacacgagac
     8581 aggtaatata cttgttaagg aacatttagt aagaggaaac gctaaagaaa agatagaaaa
     8641 ttcaaaatat gttgtaacta aaagatatac tacacctgct acagaacacg catttttaga
     8701 gcctgaatgt gcagtagctg gtccttatga agaggatgga gtaataatct attcaacaga
     8761 ccaaggggtg tttgccactc aacatgaatg tgcagatatg ttagggctac catatgagaa
     8821 ggtaagagtt ataaacaaaa tggtaggagg aggatttggt ggaaaagaag acatgagtgt
     8881 tcaacaccat gcagccttac ttgcatacca tactaagaga tttgtaaaag tacagttaac
     8941 aagaaaagaa agtattattg ttcatccaaa acgacatgcc acagaaatgg aatttacgac
     9001 agcatgtgat gaaaatggat acttaacagg tatgaaggca acgataatat ctgacacagg
     9061 tgcatatgcc tctcttggag gaccagtgct acaaagactg tgtactcatg cagcaggacc
     9121 atataattat caagatattg atataacagg aacagcagta tatactaata accctcctgg
     9181 aggagcattt agaggatttg gagttacaca gagttgtttt gcaactgaat gtaatctaaa
     9241 tttacttgct gaaatggttg gaatatctcc ttgggagata agatataaaa atgcaataag
     9301 acctgggcaa gttttaccaa atggtcaatt tgcagatgaa ggaacagcaa tggcagagac
     9361 actagaagct gtaaaagaag agtatgaaaa gcatccacgt gcaggtatag cttgtgctat
     9421 gaaaaatagt ggggttggag ttggtatacc tgatgttgga agatgtagat tggtgataaa
     9481 agatggcaaa gtccacataa gaacaagcgc ttcttgtata ggtcaagggc ttggtactgt
     9541 tatgactcaa gtagtttgtg aaactgctag cctacttcct gaaaatgtag tgcatgaagt
     9601 tccagatact catttaacac cagactctgg taatactacg gcttcaagac agacagtatt
     9661 tacgggagaa gctactaagg ttgcgtcaaa taagttaaaa gaagctttag actctggcaa
     9721 aactttactt gatttagagg gagaagagtt ttatggagaa tatgaaagta ttacagataa
     9781 aatgggttgt gacaaagaaa atccagtaag tcatgttgct tatggatatg caacacaagt
     9841 tgtaattctg gatgaaaatg gaaaattgga aaaagtagta gcagcacatg atgttggaag
     9901 agctataaat ccaatcagtt tagagggtca aatagaaggt ggagtagtta tgggtcttgg
     9961 atatgctcta accgaagatt acccagtagt taactctgtt cctacagcta agtttggtac
    10021 attaggttta tttaaatcta cagatatacc agaaatacaa gctttaatag ttgaaaagaa
    10081 caaagattta ttggcttatg gggcaaaagg tgtaggagaa atttgtacag ttcctactgc
    10141 accagcagta caaaatgcct attatgtatt tgataaagaa tttagaactg aactaccact
    10201 acaaaatact ccatatagta aaaaaaagta acaaaaaaaa gaaata
//
LOCUS       DAMJVI010000122        10149 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.127, whole genome shotgun sequence.
ACCESSION   DAMJVI010000122 DAMJVI010000000
VERSION     DAMJVI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10149
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.127"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(24..1778)
                     /locus_tag="PC545_003177"
     CDS             complement(24..1778)
                     /locus_tag="PC545_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp23"
                     /protein_id="HDF2457919.1"
                     /translation="MYIMNKKFKKFSKKLSILILVLTMFLFNNLTANAQIKEDSIIGI
                     DRYETAGLIADRQTYDTVILVNGDKNLSDGLSSSGLAGAINAPILLTKKSEIPKATLN
                     RLDNKSLNVVKKVYIIGGYNTIENSVEKDIKRKGIQVERINGNNRIETSYNVAKKINE
                     VSSVKEVMLTNGFVGEADAMSIAPVVAKNKGAIILTDGKSVPFKTEGLNVYAIGGKSA
                     INEDLVNETKAIRIGGNDRFETNKKVMEKFYNGATDFYITNGYQLVDALTLSPLAKEK
                     PIVLVADGSNKGILKGAKSITKVGGIDANIYKQCLDMIKYNNMDMTPNILKHLTSVEG
                     NEVSEILIESDNLGVVVEKTNTNKFEFDYVSVTNEKNCTFSVYKENSSNNIKNGKLVV
                     SAKKKIDKKDSDLQDMNGDNMINANKDRMVNVIKIGVPDKMYSNFKVDVESGSVELYN
                     IKGGATVDVNDGIANIVDNNVTNPFNINTNSGISGVTAETISSEIKFRANNGIVNVTA
                     TNINGNISLFGSDNKDTFDGIFKLNLKKEPSNLHLKLIGNGINKLPEGWSKDYILGNG
                     QPIIEVKNNGINNISIGE"
     gene            complement(1996..2886)
                     /locus_tag="PC545_003178"
     CDS             complement(1996..2886)
                     /locus_tag="PC545_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2457920.1"
                     /translation="MAKLTLEHIKGNTYYIPLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLNPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVRTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKSLI
                     NINKKKMEEIIDNIKKVCYEPVMIDEVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKAA"
     gene            3035..3895
                     /locus_tag="PC545_003179"
     CDS             3035..3895
                     /locus_tag="PC545_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2457921.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPNAFIIITDIHE
                     ALGNGFKKIE"
     gene            4351..4917
                     /locus_tag="PC545_003180"
     CDS             4351..4917
                     /locus_tag="PC545_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2457922.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLVVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNVRGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLEDIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            4957..5538
                     /locus_tag="PC545_003181"
     CDS             4957..5538
                     /locus_tag="PC545_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2457923.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTDGKVTNDGDFIFYNNLKHASGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(5595..6512)
                     /locus_tag="PC545_003182"
     CDS             complement(5595..6512)
                     /locus_tag="PC545_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457924.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     VISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVEYGKKQARKKSRSVNSL"
     gene            6642..8273
                     /locus_tag="PC545_003183"
     CDS             6642..8273
                     /locus_tag="PC545_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2457925.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKIIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFVRTPNDAIKLRDSIENGIKRAVVIGGGF
                     IGLEIAENLSAMGIRVSVVDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIE
                     GENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNNTIIADEYMLTNDKD
                     IYVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPE
                     LNAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQV
                     MGPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGE
                     LDSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGK
                     NSYMAQNRLRRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            8276..9223
                     /locus_tag="PC545_003184"
     CDS             8276..9223
                     /locus_tag="PC545_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDF2457926.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTVELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHEEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEVCPVKAAKLTDKGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQIDRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGADKFIEMLLSDEVLERKEEILEALLHLTGGAVC"
     gene            complement(9508..9771)
                     /locus_tag="PC545_003185"
     CDS             complement(9508..9771)
                     /locus_tag="PC545_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HDF2457927.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQRNYD
                     YYGGFAKKNPCRMRVPNTGTWYLVVNQDGNSGIVNFSINTIQN"
     gene            complement(9870..10115)
                     /locus_tag="PC545_003186"
     CDS             complement(9870..10115)
                     /locus_tag="PC545_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457928.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTVYKDESSDSG
                     YYALLENKEERVVWDGEVVANNIFNNLWIVINKVKTG"
BASE COUNT         3540 a         1459 c         1422 g         3728 t
ORIGIN      
        1 ataaacttta ttttcatatg tttttattct ccaattgata tattatttat gccattattt
       61 ttaacttcaa taattggttg tccatttcct aaaatataat ctttagacca accttctggc
      121 aatttattta tcccatttcc aattaacttc aagtgtaaat ttgatggttc ttttttcaga
      181 tttaatttaa atattccatc aaaagtatct ttattatcgc ttccaaataa acttatattg
      241 ccatttatat ttgtagctgt tacatttaca attccattgt ttgctctaaa ttttatttca
      301 gatgatatag tttcagctgt tacacctgaa atcccactat tagtatttat attgaaagga
      361 tttgttacat tgttatcaac aatgttagca attccgtcat tgacatcaac agtagctcct
      421 cctttaatat tataaagttc tactgaacca ctttcaacat ctactttaaa attagaatac
      481 attttatcgg gaactccaat ttttatcaca tttaccatcc tatctttatt tgcatttatc
      541 atattgtcgc cattcatatc ttgcaaatca ctatctttct tatctatttt tttcttagca
      601 cttacaacta gctttccatt ttttatatta ttagaagaat tttctttata tacagaaaaa
      661 gtacaatttt tttcattagt tacacttacg taatcaaact caaatttatt ggtattcgtc
      721 ttttcaacaa ccacacctaa attatctgat tctataagta tttcagaaac ttcatttcct
      781 tcaactgaag ttaagtgttt taaaatattt ggagtcatat ccatattatt atatttaatc
      841 atatctaaac actgtttata tatattggca tcaatacctc caacttttgt tatactttta
      901 gctcctttta aaataccttt attacttcca tcagcaacta aaacgattgg tttttctttg
      961 gctaaaggag ataatgttag tgcatctact aattgatatc catttgttat ataaaaatct
     1021 gtagctccat tataaaactt ttccattacc tttttattgg tctcaaatct atcatttcct
     1081 ccgattctaa ttgctttagt ttcatttact aaatcctcat ttatcgcaga tttgccacct
     1141 atagcgtaaa catttaaacc ttcagtctta aatggaactg atttcccatc tgtaagtatt
     1201 atagctcctt tattttttgc aactacaggt gctatactca tagcatcagc ctctcctaca
     1261 aatccatttg taagcattac ctcttttaca ctacttactt catttatttt tttagctaca
     1321 ttataactag tttcaattct attgttacca tttattcttt ccacttgaat accttttctt
     1381 tttatatctt tttctactga attttcaata gtattataac caccaattat atatactttt
     1441 tttacaacat ttaaactctt attatctagt ctatttaaag tagcttttgg tatttcactt
     1501 tttttagtca aaagtattgg tgcgttaata gctccagcta atccacttga tgaaagccca
     1561 tcagataaat ttttatctcc attaactaat attacagtat cataagtttg tctatccgct
     1621 ataagtcctg ctgtttcata tctatcaatt cctattattg aatcttcttt aatctgtgca
     1681 tttgcagtta aattattaaa aagaaacata gtcaaaacta aaattaatat acttagtttt
     1741 ttactgaatt ttttgaattt tttattcata atatacacta ttcctcctgt aaattaaggt
     1801 ctttaatttg cttataaatt atccccttat ttacaaataa taacataata ttatagtaac
     1861 gtatatatta ccttaaaatt atcagtaact ttatatcaat catcttagct tatttatata
     1921 aattttttta tattactttt caaataacag tcatactaaa acaatttcat catataattt
     1981 gaaaattatt actatttacg cagcttttat catcatctta ccaccatcaa atatatattc
     2041 aaccatatta ttctcatata aataagttat ataagaacgt attgtacttc caaccaacac
     2101 atattgatta gcatcaagct tgacattgta caaatcacac attttttcaa taacttcatc
     2161 aatcataact ggctcataac aaaccttttt aatattatct attatttctt ccatcttctt
     2221 cttattaata ttaattaaac tttttatatc tgtagttttg acagagtgac ttggaacaaa
     2281 gaaatcagcc tctaacattc tcaatttttc tagtgtctta aactgtgaat ctatatccag
     2341 taggaagaaa aaatgatatt tagtaattat attctctggt gtaagactat ctgcaacaaa
     2401 caaaacatta tctggagttc taacaccaat catttcaaaa taatgacctg gaagagaaat
     2461 cgcttcaagt tctgtatcta caattttgcc atttgaaggt ataatataat caacttcact
     2521 ttcctttgct aataaaaatt tattcttcag tgctttactt ggatagccac catataaaaa
     2581 tgctgattct aatattggat tttctgtaaa atatccttca attttagtcg tagcaatctt
     2641 gcattttgtt tgattcttca aatatgcatt tcctcctata tggtctgcat ttgaatgagt
     2701 gtttattatt aatttaggat ttaaattatg ttcttctaat aatcttaaaa cttgtctagc
     2761 tgtatcctta ttatttccac tgtcaattaa aattgcatcc ttaccatcta cgtatacacc
     2821 tactattgta gggagtggaa tataatatgt atttcctttt atatgttcta gagttaattt
     2881 agccatttta taacctccct ttaaatttac ttttaattat agcattaatt ttctgaaaat
     2941 tccaatcaaa ttgtttttat aatatatttt tatttctttt attaaaaatt ggaaaaacta
     3001 tataatatat attataatct atatataagg agtgatgtta ttgaaaaaag atagaataac
     3061 tttttcagaa attttaatat taattgtagg ctgtatatta atggccattt ctcttaactt
     3121 attttttaat ccacatgcaa tcgctgctgg tggtataaca ggacttggag ttgttttaaa
     3181 ttcattattc ggtgtagaac tttggattgt caatttactt ttaaatgttc cattattcat
     3241 atttgcttac aaaatacttt caaaaaaaga ttgttttaaa acggttttag gaataatttt
     3301 tcttaccata gcacttaagc ttactgcaaa tatggctaca ttagatataa caaatgatat
     3361 gtatcttgca attatatctg gttctatttt aatgggagtt ggtcaaggac ttatttttag
     3421 aataaatggc tctacaggtg gaactgattt aatggcactc ctactaaata aatattttcc
     3481 tacatttagt atacctgttt taatgggtat tgtagactgc gttgtagttg ttttatcagg
     3541 tatagtaaat agacaggttg aaattgcact ttattcaact gtggctttat acattttagt
     3601 aaaagttagt gacttgctta ttgaaggatt taattattcc aaatcattca ctattatatc
     3661 tgacctttca aaagatataa gtaaaaaaat aatggaagat ttagatagag gtgcaactat
     3721 tctaaaagga gaaggtgcat atactggaga aaataaaaat gtacttttag tagtagttga
     3781 aaagaaagaa gttgtcgaat tgaaaaaatt agtaaaaaat gtcgacccta atgcttttat
     3841 tataattaca gatatacatg aagctttagg taatggattt aaaaaaatag aataactaac
     3901 aaataaatag caagtataat taacatatac aaattcaact ttttatatgt tatattatac
     3961 ttgctattta tatattatac ttgctattta tatattataa ttgttgtttt taagataaaa
     4021 attaaatgta tcaattattg ttttatacaa gtatttccaa atccgttctc atttagatag
     4081 tatgcttaga aattttagaa ttattgagtc taactttatt acttacttta ctttataaca
     4141 atcaaataca aatttaaata ttataaaact acaaaataat ttaaagcata taatgtcaat
     4201 atgtttaaat tataaaatat tcatgtagtt atataataaa taatttataa atatagccaa
     4261 cttagcctaa atacgtatat aatatagcta atatggttct tatacttgat accatcaata
     4321 aaactaaaaa tataaaaagg agaacttaaa atgagtgtta gtttacaaaa aggacaaaga
     4381 gttagcttag taaaagataa taatcctgta aaaaatctag tagtaggctt aggatgggat
     4441 atgaataaat tgggtaaaaa aaattatgac ctagatgcat ttgcaatagc attgactaac
     4501 caagataaaa tgaatgttag aggtgacttg gtttattttg gaaatcttaa acacccttct
     4561 aaagctatta tacacactgg agataactta actggaaaag gtgaaggtga tgatgaacaa
     4621 ataattgtta atttagaaga catacctgaa tatgtacata aaatagtttt tggagtttct
     4681 atatacaaag ctaaaaaaag agaccaggat tttggtcaaa tgaacaactc ttttataaga
     4741 ttaattgatt caaattccaa gcaagaatta tttaaatata atcttcaaga agacttttct
     4801 ggtaaagtaa cagttttggc aggagaaatt tatcgtagaa atgaagaatg gaaatttaat
     4861 gctttaggaa ttggtcaaaa tgaagagctt cgtgaattaa ttaatacata taagtaataa
     4921 ttacaataaa aaatttataa tgaggagttg gtttaaatgg gtataacttt agcaaaagga
     4981 caaaaagtaa gtttaacaaa aagtaaccct ggtctaaaaa aagtaatagt aggactagga
     5041 tgggatatta ataaatatga tggtggcttt gatttcgatt tagatgcctc tgcattttta
     5101 actggaactg atggaaaagt aacgaatgat ggagacttta tattctataa taatttaaaa
     5161 cacgcttctg gagctgttga atatatgggt gataatagaa caggtgttgg tgatggtgat
     5221 gatgaacaaa taaatgtaga cttatctaaa atacctcaaa atattgctaa aatatcattt
     5281 agtgttacta taaacgaagc aattacaaga agacaaaatt tcggacaagt tgaaaattca
     5341 tatataagaa tatataatga agaaactaat gaggaactta ttaaatatga attgggtgaa
     5401 gattttagca tagaaacagc aatagttgtg gcagaactat atagacataa tggagaatgg
     5461 aaatttaatg ctttaggttc tggatttgag gatggacttg caggattgtg taaaaatttt
     5521 ggcgttaata taggctaata tatctaaata aaaataagag tatattgata tactcttatt
     5581 tttatttaga tatattataa actgtttaca cttctactct tctttctcgc ttgttttttc
     5641 ccatattcta ctgaaatatc aaaaaatgct gaaatatttt ctgtaaaaaa cttgcttcta
     5701 tgatatgcaa tatcaaatgt cctatgtaaa ttaatacctt caatatcaca agcccacaaa
     5761 agatgctttt ttaaataatc ctcaataagt aattcggata taactgcaat gccaaaatta
     5821 tctacaactg ctcttaatat agcttcagag ttataacatg accatctaat attcattggg
     5881 atttttaatt cttttaattg cttttctaat tgtgctcttg taccacttcc taattcacgc
     5941 ataattaatg gttgatttga aagttctgaa acttttatag agtctctttt ataaaactca
     6001 tgcttgtgtg aacatataag tacaagatta tcattaataa tgctttttgt aacaatatct
     6061 gggtgcttaa tttttccttc tacaatcgca atatctaatt cactattaag taattttttt
     6121 gcaatcagtc ttgtatcttc tacaaatact tcaggttcta taagaggatt taccttgtat
     6181 aattctaaca ttattggact tattacacat gtaccaacag ttactgttgc cccaactctt
     6241 atacggatat tttttgcaca atgcttcata caatcattca tttcttcatt tacagctaaa
     6301 agatgcctcc catatccaag aagttttttc cctgcctcag ttaaatataa tttttttgaa
     6361 attctatcaa atagcttaac accatactct ttttctattt ctgaaatagc ttggctaact
     6421 gatgactggg taatatacat atttcttgct acttcactca ttttaccaca ttcagcaacc
     6481 ttaacaaaga tttcaagact acgaattgtc atattatcac aaccttagca aacattagta
     6541 aatgcttatg attttattaa tattttagta ttttacaaat atattgtcaa gaagtataat
     6601 tttattatag agttataaag tagattggag tgtgatatat tgtgaaagta ttaattatag
     6661 gtggagttgc tgctggaaca aaaactgctg caaaaataaa acgtgaaatg ggtgataatt
     6721 gctctgttac aatactaaat aaaggtgatg atatttctta tgcaggttgt ggtcttccat
     6781 attacgttgg taagataatt gaagataaat catctttaat cgtaaataca cctgaaagtt
     6841 tttctaaact aacaggagca gaagttcgtt gtggagttga agttacatct attgatagaa
     6901 atgcaaaaca agtaactttg aaaacgcttt caacaggaga aaaagaaaat ctaagctatg
     6961 acaaacttgt aattgctaca ggggcagacc caataaaacc accaatacct ggaatagatt
     7021 tacctggagt attctttgtg cgtactccaa atgatgctat aaaactaaga gattctattg
     7081 aaaacggtat taagagagct gttgttattg gtggaggttt cattggtctt gaaattgctg
     7141 aaaatctttc tgcaatgggt atacgtgtaa gtgttgttga tatggctgaa catgttatgc
     7201 ctggatttga tactgatttt gctgaatacg ttgaaaatca tatggctgat aaaggcataa
     7261 tgatttttac aggtgaccag gttgtaggta tcgaaggtga aaataaagtt gaaaaacttc
     7321 gtactaaaaa tagagctatt aaaacagatt tagtggttat gtcagttgga atccgtccta
     7381 acacaggttt tctaaaagac acaggtttag aatttgcccc taataataca attattgcag
     7441 atgaatatat gcttacaaat gacaaagata tatacgtagt tggagactgt gcttttgtaa
     7501 aaaattcttt aacaaataaa cctacatggt cacctatggg gtcatctgca aatcatgagg
     7561 gtagaatttg cgcacaaaat atatctggaa aatctaaaac ttacaatggt gttcttggaa
     7621 caacagtagt aaaattacct gagctcaatg caggaaaaac aggtctaagt aaagaaatag
     7681 ctgaaaaaga aggttataat gtttgctctg taaccatagc tactgatgat aaagctcatt
     7741 attatccagg tgcatctaat tttattataa gaatggttgc tgaaaaaaat actagaaaat
     7801 tattaggtgt tcaagttatg ggacctggag cggttgataa aatagtagat atcgctgcta
     7861 ctgcaataac tttaggcgct gacctttatt cattagaaag tatggacctt gcatatgcac
     7921 cacctttttc aacagctatt cacccttttg tagtggcagt aaatgtttta caaaataaac
     7981 ttaatggaga acttgatagt gttttacttg acgaaattga ggattttgac agttggacta
     8041 aacttgatgt ttcaaaagct ccttctattc ctagtttacg ttatattcct gttggtgaaa
     8101 ttaatggaga aattgaaggc cttgctaagg atgaaaaaat tcttcttata tgtgctaaag
     8161 gtaaaaatag ctatatggcg caaaatcgct taagacgctt tggctataca aatacgaaag
     8221 ttcttgaagg tggaatacta ttttatccaa atcttaaggt aatggaggag taaatatgag
     8281 taatgttaca gcagaacaaa aaaaggaagt aaaaggtaga ggttttctac caagcaggga
     8341 tggagaacat tttgcagcac gtataataac tgtaaatggt gtaataaatg catcacaagc
     8401 aaaaaaaatt gcagaagctg ctgaaaaatt cggaaacggt cagttagcat ttactactag
     8461 actaacagta gaattacctg gtgtaaaatt tgaagatatt gaagctctaa gtgaatttat
     8521 tgctagcgaa aatctaataa caggtggaac tggatcaagg gttcgtcctg ttgtggcttg
     8581 taaaggtact gtttgtgttc atggtctagc tgatacacag gcacttgctg ctgaaataca
     8641 cgaagaattt tacaaaaatt ggtatgatgt aaaactacct cataagttca aaattggtgt
     8701 tggtggatgc cctaataact gtattaaacc tgacctaaac gatcttggaa ttgttggtca
     8761 acgtgtacct gactatgatt cagagctttg tgttggttgt aaaaaatgtg ctgttgtaga
     8821 ggtctgtcct gttaaagcag ctaaattaac agataaaggt aaacttgaaa tagatagtaa
     8881 tctatgtaat aactgtggta agtgtataga aagttgtaat tttgatagta ttgaagaaaa
     8941 agaatctggt tataaagtct atataggagg gaaatgggga aaatcagttc gtcctggaac
     9001 acagattgat agattatttt caaaagagga acttatgact ttaattgaaa aagcaattct
     9061 tttatttaga gaacagggta acacaggtga acgttttgga ataactatcg atagaatcgg
     9121 tgctgataaa tttatagaaa tgcttttatc agatgaagtt ttggaaagaa aagaagagat
     9181 actagaggct cttttacacc ttacaggtgg agctgtatgc taacaataaa tattttaatt
     9241 gtacagataa cctatggaaa aaattttagt taagaataat taaaattcta tatatgatat
     9301 atgataaatg aggctatctc aaaacacaga tacgcctttt tttcattgtt gaaatgataa
     9361 agtatatttg agttcatata aaaaagagtg tctcgtgaat tataaaattc attttgaggc
     9421 acccacatta ttttataata attgcttatt aattttattt taactatata aaaagtacaa
     9481 aaagtaagaa tatccaatat tctaaaatta attttgaatt gtattgattg aaaagtttac
     9541 tattccacta tttccatctt gatttactac tagataccat gttccagtat taggaactct
     9601 cattctacat ggatttttct ttgcaaatcc accatagtaa tcataatttc tttgattttt
     9661 aaatttcaca taatttatat ggtctaacaa tattacattg gcttgtttct ctaaatttac
     9721 ctctacaata tccccttttt ttaagttcat cttactataa ctatattcca ttgaattact
     9781 cctccagtat cgaataatct ttgttattta ctcatctcaa atatgtaaaa attcatttaa
     9841 aatattacaa atactatata attagactat tatccagttt taaccttatt tattacaatc
     9901 caaaggttat taaaaatatt attggcaaca acttctccat cccatacaac tctttcttct
     9961 ttattttcta ataaagcata gtacccagag tcagaacttt catctttata gactgtaaca
    10021 taaaactctt catcactata ttcaggtata tttttaaagt cttcagatat tttacatgtt
    10081 aatatataac cttctaattc ttcatctata aacaaagcaa ttcctccaat ctctatattt
    10141 ttactaatt
//
LOCUS       DAMJVI010000123         9613 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.128, whole genome shotgun sequence.
ACCESSION   DAMJVI010000123 DAMJVI010000000
VERSION     DAMJVI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.128"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            130..801
                     /locus_tag="PC545_003187"
     CDS             130..801
                     /locus_tag="PC545_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2457929.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQIIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKECLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDDNIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            803..1711
                     /locus_tag="PC545_003188"
     CDS             803..1711
                     /locus_tag="PC545_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2457930.1"
                     /translation="MFILAFIICTVVIFIVSTKINRKRYDELNLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKSEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRINLEPFSDIKLVHNKKWTIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVENQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            1788..2474
                     /locus_tag="PC545_003189"
     CDS             1788..2474
                     /locus_tag="PC545_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457931.1"
                     /translation="MSILVTNNLVKHYGKGETKVKALNGVSIEIERDTFTAIVGTSGS
                     GKSTLLNIIGGLDNPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGELL"
     gene            2471..5026
                     /locus_tag="PC545_003190"
     CDS             2471..5026
                     /locus_tag="PC545_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2457932.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKEYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETALEGFVNDYFQPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     TYINWSLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILIAVRVVHQLNKYSSIESMKNFKITNKKK
                     RILESVKHSNMTKVLSHKYMTQKKGMFIGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNVEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKTMKPTDEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFDIENYNSVSIKKTEDSNS
                     EELSENIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFLLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINTPLYIGMIILNITISIVAVIIPVRQILKSDIISEINKN"
     gene            complement(5220..6419)
                     /locus_tag="PC545_003191"
     CDS             complement(5220..6419)
                     /locus_tag="PC545_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HDF2457933.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            6538..7425
                     /locus_tag="PC545_003192"
     CDS             6538..7425
                     /locus_tag="PC545_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDF2457934.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENEKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            7615..8172
                     /locus_tag="PC545_003193"
     CDS             7615..8172
                     /locus_tag="PC545_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2457935.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKRDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(8411..9271)
                     /locus_tag="PC545_003194"
     CDS             complement(8411..9271)
                     /locus_tag="PC545_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDF2457936.1"
                     /translation="MKKQKSPILLEMLGLFFGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
BASE COUNT         3737 a         1160 c         1410 g         3306 t
ORIGIN      
        1 tataatgttt ttattttgaa aacaaattta aatacaatct ttttagtgaa ataaataaaa
       61 ttaaaatatt tttagtatga taatgcaaga aaataattat ttatctaaaa aatctaaagg
      121 agagtaaaat tgaacttatt aattattgaa gatgatataa acttaaatga aggtcttttt
      181 tatgcatttg agaatgatgg atttaatgta tttaaggctt atacaaaaca agaaggatta
      241 aatatattta atagtaaaaa tatagatttt ataatcttag actgcaatct tccagatgga
      301 gatggatttg atgtatgtca aataataagg gaaaagtcag atataccaat tataatgctt
      361 actgcaagag actcagaaat tgatgaagta aaaggattag aaatagggct tgatgattat
      421 attacaaaac cattttcttt atctgtatta aaggcaagag tcaaagtagc aataagaaaa
      481 aaatcgaata ataaagtaat atattccaat ggaataaaat tggaccaaaa gttactaaaa
      541 gtatataaaa ataaagaatg tttggaactt agctctgtag aatataagct tctaagttat
      601 ctaattgaaa ataaaggtca aatattatta aaggaacaaa ttttacacca tatttgggac
      661 agcgaagaaa attatgttga tgataatatt gtatctgtaa atataagaag acttagagta
      721 aaagtagagg atgacccatc taatccaaaa tatataaaaa cagcttatgg aatgggttat
      781 ttatggaatg aggtagaata atatgtttat attagccttt ataatatgta cagttgttat
      841 atttatagta tctactaaaa taaatcgaaa aagatatgat gaattaaatt tgatgttaaa
      901 tcaaatattg gaaggtaaag aagttactta tccagataca aaggatacaa gagcgtcaaa
      961 aatttctcac caagtaaaaa aaattaagga tatgatagaa atagaggtag aacagtcaaa
     1021 gtcagaaaaa gaagcgatta aaggtttaat atcaaatatg tcacatcaat taaaaactcc
     1081 actttcaaat ataaccattt attgtgaact tctggagaat ataaatattt ccacattaca
     1141 gaagaaggag tttttacaaa agatgaaaaa tgaaactttt aagattgact ggctgttaca
     1201 atccttattt aaaatgacta aattagagga tggagttata gaatttgaag tagaagaact
     1261 actaattaaa gatactctta ttcaaagtat cagtactatc tttaataaag cagaagctaa
     1321 aaatataagg ataaatctcg aaccattttc agacataaaa ttagttcata ataaaaagtg
     1381 gaccatagaa gcaattgtaa atgttttaga aaatgcaata aaatactcac catcagatag
     1441 cacgattact atttctgtaa taaaaatgga actatatacg aagattacta ttaaagatga
     1501 gggaataggt attgatagta gagaactaaa tgatattttt aaaagatttt acagaagtaa
     1561 aaatgttgaa aaccaaaatg gcacagggat tggtctatat cttacaagat taattttaga
     1621 aaaagaaaat ggaaacatta tagtagaatc taaactagga agtggatgtt gttttagtat
     1681 cttcttacag aattgtaaga gtttaaacta attttgtagg atgagtgtaa ttatcgtcac
     1741 ttacaatatt ttatagaaca tatatctgta ttgaaaggag aaattaaatg agtatattag
     1801 tgacgaataa tttagtaaaa cactatggaa aaggtgagac taaagtaaaa gccttaaatg
     1861 gagtaagtat agaaattgaa cgagatacat ttactgctat agttggaact tctggttctg
     1921 gaaaaagcac attgttaaat attataggtg gattagataa tccaacctca ggtgatgtaa
     1981 taattaaagg aaagaacata tctaaaatag gaaaaaaaga tttgacagtg tttagaagaa
     2041 gaaatatagg atttatattt caaaactaca gtttaatgcc cgtattaaac acttatgata
     2101 atatagctct tcctgttaca ttagataaag gaaaccatat agaccatgaa tatattgaaa
     2161 tgctgatgaa gacattggga atttgggata agcgtttaaa atttccaagc gaactatctg
     2221 gtggtcaaca acagagggta gcaattgcta gagctttggc aaataaacct gcgcttatat
     2281 tagcagatga accaacagga aatctcgata gtaaaactac aatggaagtg gtatgtttat
     2341 taaaagaaag tagtgcaaaa ttccatcaaa cgatacttat ggtaacacac aatgaaaata
     2401 tagcacaagt atgcgactca attatccata ttgaagatgg agttgtggtt aatactggtg
     2461 gtgaattact atgatgtctc tagtaaaatt ttcaattcaa tatataaagc attataaaaa
     2521 acaatctata tctatagttt taagcatagt actatcagta gcattattga cgggaattgg
     2581 ttctttagtt catagtgcag acaaaagtag aattgaaaag ataagggaag atagtggaga
     2641 ttatcattat ttctataaag tagataagga acaacttaat aaaattaaag aaaataaaaa
     2701 atcaaaggaa tacactgtag atagacttgg cgttacaaaa cctaaaaatc agatagatga
     2761 accttttatt atagaatttt taaatgctga ttcttcatat ttagacatga caggtagaaa
     2821 attattaaaa ggaactcttc ctagtaaaga tggagagata gtacttgaca catattctct
     2881 aaacaattta aatgtaaaca aagaaattgg aacagaaatc aatttagatg gtaaaatcta
     2941 taagttatgt ggtattttat caagtctttc aaacccagaa actgctctgg agggatttgt
     3001 aaacgattat tttcagccta aagaaaagga ttcttatata gtttatgtaa aatatgatga
     3061 aaataaaaat attaaaaagc aaaattataa atttatgaaa aaattcaaca tatcaaaaga
     3121 aaaaacttat ataaactggt ctctttcagt tgaatttgga gtaaaaccac caaaaaatat
     3181 agaaaaatat aatatatttg agttcttaaa aaatttagaa ctaaatacaa atgcaattat
     3241 attgcttgtt ggagtgtttg gtgcagcagc agtatatagt attttccatg tttcaatact
     3301 tcagagaata tcacaatatg gggttttaga agtattaggc gctaataata aacaactttt
     3361 atttctacta tttttagagt tatttttact ttttattgtt ggatttccta taggttgctt
     3421 cttaggtatt ggagtagcgt ctacaatata tgaacaattt ccacatatat ttttgagtag
     3481 tgatattgtt ccaggagcat tttttataag tgaaaaatct atttatttag gctttttatt
     3541 tatcttattg ttgttaattt taattgctgt acgtgttgta catcagttga ataaatactc
     3601 tagtatagaa tctatgaaaa actttaagat tactaataag aaaaagagaa tattagaaag
     3661 tgtaaaacac tctaacatga caaaagtatt atctcataaa tacatgactc aaaaaaaggg
     3721 aatgtttatt ggaattttgt tttcattatc acttggtgga attatatttc tatgttctag
     3781 ttactctata cagcttacta aaaataataa tgaacttaca atgaaagctg atgatggttt
     3841 aaattctgac taccaaataa ctatgcaaac aacagatttt gatgtaggta taccaaaaga
     3901 taaaatttca agattaaaca atgttgaggg aatatcaaaa gtctatccag taagatattt
     3961 atttggagct atgcaaatta aagaagaaca actattttgg aaaaatttct ttaaacctct
     4021 tgaaaaagat tacaggataa agactttttt tgatggtatt tgtacaaagc aaaatgaagg
     4081 tggctatctt ttaaaaacaa atatttatgg ttataacaat ggtatgttag aaggattaaa
     4141 ttcttatatt atagatgggg agattaatac ccatgatatg gttaaaaata ataaggttat
     4201 agttagacta cctatggatg gaactgggat gtatgatgca gttgatataa atccaggaga
     4261 tacaataaaa gttaaagtgc caaagacaat gaaaccaaca gatgaaattg ttaaatttaa
     4321 agaagaaaat aagaatgatt atacaacaaa agaatttgta gttgctgcta ctgtaaaacg
     4381 tgtgatggca aataacatat atttcattgg tgattatggt atggatatag ttatgacgaa
     4441 tgggcaaatg aaaaataact ttgatatcga aaattataat tcagtaagta taaaaaagac
     4501 agaagatagt aacagtgagg aattgtctga gaacataaaa agtgttgtaa gtgatgtaaa
     4561 aagatgtatc gtaacagatt ataccattgc tattgaaaaa aataatacct acttaaatca
     4621 aaaactctta tttatatatg gtattgtatt tgtacttctt gcaataagtt tgttccatat
     4681 aatcaataca gtaagctact taattttttc aagaagacat gagtttggaa ttttaagagc
     4741 tatgggtatt acggataata agtttttact tatgatgata agagaaggtt ttttatatgg
     4801 attctatgca agtattataa tggtaattgg aagtgttatt ggacaattta tgatttattt
     4861 tatggtaaaa cgtgtatatt tatatataaa tccaattctt aaaataaata cacctctata
     4921 cataggtatg attattttaa atatcactat tagtatagtt gctgtaatta ttccagtaag
     4981 acaaattttg aagagtgata ttatttcaga aataaataag aattagagtt atataaattt
     5041 ttaagattat ataactttct gacaatcttg tttaaattat ttaaatatat cttatctgtg
     5101 aacattaaat aaacctctta atagatataa ctcttgaaaa gtcatatcta ttaagaggtt
     5161 ttaataaatt ttcatgttaa tatatataat ccaaaaaaat taaatcttat taaaacatac
     5221 tattttgtat agttatcgaa atatccttgt atgtaaataa taggagtacc cttatctcca
     5281 cttccagatg ttaaatctgc taaagaacct ataaggtctg ttaatcttct tggagtagta
     5341 ccttgagaaa ccatgtttcc tgttaaatca tcagatttat tatctttttc aactatgtat
     5401 tttgaaattg cttcttttaa ctcatcacca gataagtcag caaaatcatt atctgctaag
     5461 tattttaatt ttacttcgtt tggagttccc tctagccctt ttgtataagc aggagatact
     5521 actgggtcag ctaattccca tattttacct acagggtctt taaaagctcc atctccataa
     5581 accattactt ctacatcttt ccctgttatt tcttttatat taccttggat tttgtttaca
     5641 acttcatcac aatttatagg gaataatttt actgtttctt ctgtagcttt gtttgaacct
     5701 aaaagtccat actggtcatt gtaaccacta ccatctacac ttgctgtcat tatatcatca
     5761 agtgaatata cttttttagc tccattttgt cttaaaattc tctttgttct ttgtcttgta
     5821 tgtatatcac aagttaaaat acttgttgta tagttaagaa tagtttttgg attgtttgca
     5881 aatactattt ctacctctgc accacaatcc ttgattaaat tcttataata atcaacataa
     5941 tctacaccag taaatgtatg cttgttatat ccaaatagtt ctctgtattt ttcttctgtt
     6001 aaaacatctg accaaggatt tattccctta tcatcaagtt catcaatact tattaagtga
     6061 tttccaactt catcagaagg gtagcttaac attaatacga cttttctaca tccttttgca
     6121 attcctttta aacatatagc aaaacgattt ctacttaaaa ttgggaatat aaccccaaca
     6181 gtatcatctc caaatttatc ttttacatct tttgcaatat tgtcaacgtg agcataattt
     6241 ccctgtgctc tagctacaac agcttctgta acggcaatta catctttgtc cctaacttca
     6301 aagttttcac tttttgacgc atttaaaaca gaatcaacaa ctattttaac taagtcatct
     6361 ccttgtctta taataggtgc tctaacccct cttgaaacag ttccaactaa tctatccatt
     6421 atctaaaatc ctcctaaaga attaatattg tcatattagt accatgtata ttataattca
     6481 aatataatta taagtaaaat aaataatact aatatttgac ataaggagta attatatatg
     6541 aatataaaac ttgaacttta taaggttttt aatgcagttg taaataataa gagtttttct
     6601 cttgctgcta aggaattgtt tatgtcacag ccagctgtaa gccagtcaat aaagcaatta
     6661 gaagaacaat tagatacttt attattttat agaaacaata agggagtaaa attaactcca
     6721 gaggggaaaa ttctaagtga acatgtcact actgcattaa agttaatatc atcaggagaa
     6781 gatagaataa ataaatttaa aaaattagaa tacggttctc tgaaaatagg agtaggagat
     6841 acagctgcac gttttttctt gttaaaatat ttagaaattt ttcataagaa gtatccacat
     6901 atacatgtat ctacaataaa tagaacgagt agagaactta tatctttact caaagatggg
     6961 aatatagata tagcaattat aaacatgcca atagaagatg atacactaaa tattgttgag
     7021 tgtattgaaa tacatgatat atttgtatgt gcaaatgatt acatagaata caaaggtagg
     7081 aaaatatcat tggaagaatt aaacacattg cctttaataa tgctagaaaa taaagcaaat
     7141 tcaagacttt atgtaaatga atatttttta tcaaaaggta taaaactaaa cccggatata
     7201 gaattgggtt ctcatgaatt acttttagaa tttgcgtata taaatcttgg agtgtcttgt
     7261 gttatagaag aatttagcat agattatttg gaaaatgaga aattgtttaa attagatata
     7321 aaagagccaa tacctaagag aaatataggt tactgccatt taaaagatat atctttatcc
     7381 ttagcaacca aagaattttt aagcatgata tctaataata tataatttag aatacatatt
     7441 tcacaagtaa tttaatataa aaatagagat aaattcgtaa atttgaaaga ttcatatagt
     7501 aattagtttt tattaaaaat aaaaataagg ttaatttgag aacatttata taattaatat
     7561 cgtattttaa atatgaacag tatatttaga ttaatgtata aagggggatt agaaatgtct
     7621 gattctgaat atcctcaaat gaaaacctta gaaactagaa attgtatact aagacctgca
     7681 tcattagatg atgttgaaga tttttttgac tgttataaaa ataaaatagt agtaaaacat
     7741 ttaccattta gtgagcataa atctttagaa gatacaagaa agtttataaa atcatttttt
     7801 ctaaatccat ataaaaaagg gaaaattggc cactttgcta tagtatataa aagagacaat
     7861 aaagttattg ggaatatggg gtttaataat ataaatccta aagcattaga agcagaaata
     7921 ggtatatgta taaatccaag ctattgggga catgattttg ctactgagat aacaaaaaga
     7981 gtaatacagt atggatttag agaactaaac ttaaataaaa taattgcaat aacctatgaa
     8041 gaaaaccaaa attctacaaa atcattagat ttattaggat ttaaatgtac aggaaagtat
     8101 atcaaaaaaa ttcatactgg aaatactgtg aaaaatgttc cttgttatca atatgaattg
     8161 aaaaaacaat agatataatc atttaatatc tatgctggtt atttagtaat gattgttttc
     8221 taaataaaat ttcaataaag tggttgcctc aaaatataga taatcctgtt tttattgtta
     8281 aaatactgaa aatgcatttg attttataca aaaaagagtg tctcatgaac taaaaagttt
     8341 atgagacact ctttttgaat aaatcgtatt ttaattaaaa tagtatatat acttaaaata
     8401 ttaattttta ttagttctct aaatttttaa atccttctcc aagaacctca tgtacatctg
     8461 taacaataac aaatgcagta gggtcagttc ttttaactaa tctctttaga taaagttctt
     8521 gttttttaga aactacgact aatagaactt ctttattttc tctagtgtat ccacctttac
     8581 catcaagaat agtaagacct ctatcaagtt cttcagttat agcttgtctt aaactttcag
     8641 gctcatcaga gataataaaa aatgcttttg aatggtcaaa tccttccatt atcatatctg
     8701 ctatttttat taatacacaa agtgctatac cagagtaaag agcagtttct aaactacgat
     8761 ttactatacc agaagatata acaaccattc catctaggca tgacatgaat tttgtagctt
     8821 ttattcctgg gaatttctta ctaagtatga gtgcaattaa gtctgttcct ccagtagaag
     8881 catctgattt aaacattatt cctaaaccta taccaactag gattcctcct gcaatagaag
     8941 acaataataa gtcattagta aatcttagtt ttgataatgg gtctgttaat tgaactatag
     9001 ctgaaaatac aactgttcca aataaagttt ttaatgaatt ttttgtaccc ataattctaa
     9061 aagcaagtat tactaatggg acacctataa ttaacatgat agctgatact ggaagtcctg
     9121 taaccttgtt tagtactaat gatagaccac taagtcctcc aggagcaatt gtatgtggtt
     9181 ttaaaaacat atttatacca atactcattg atatacaacc aaaaaatagt ccaagcattt
     9241 caagtaaaat tggcgacttt tgttttttca tataaacacc tccatgaact tgtcacaatt
     9301 taatattata acatatttga ccagtttata ttatataaaa tttcttgata taagcaattt
     9361 caagtatttc attattctta atatttttga atttttatat agtgttttca tatttttact
     9421 gatgttgtta ctctcctatg aagtttttat atattataat ttttttattc ttttataaag
     9481 tatcccctat aaagatggtt ctatgaaaat tttctctata ctgtttaacg tatatagaga
     9541 ttttgtgtat aattataagg tatttaatgg aaaaatttat cttactttta tatgttatca
     9601 aaaggattta tta
//
LOCUS       DAMJVI010000124         9451 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.129, whole genome shotgun sequence.
ACCESSION   DAMJVI010000124 DAMJVI010000000
VERSION     DAMJVI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.129"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            491..1696
                     /locus_tag="PC545_003195"
     CDS             491..1696
                     /locus_tag="PC545_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDF2457937.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTEHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     IVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLMSRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1767..1988)
                     /locus_tag="PC545_003196"
     CDS             complement(1767..1988)
                     /locus_tag="PC545_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457938.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC545_003197"
     CDS             complement(2129..2728)
                     /gene="recR"
                     /locus_tag="PC545_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDF2457939.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2741..3091)
                     /locus_tag="PC545_003198"
     CDS             complement(2741..3091)
                     /locus_tag="PC545_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDF2457940.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC545_003199"
     CDS             complement(3150..4787)
                     /gene="dnaX"
                     /locus_tag="PC545_003199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDF2457941.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4864..5128)
                     /gene="ffs"
                     /locus_tag="PC545_003200"
     ncRNA           complement(4864..5128)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PC545_003200"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5166..5257)
                     /locus_tag="PC545_003201"
     tRNA            complement(5166..5257)
                     /locus_tag="PC545_003201"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5221..5223),aa:Ser,seq:gga)
     gene            complement(5258..5713)
                     /locus_tag="PC545_003202"
     CDS             complement(5258..5713)
                     /locus_tag="PC545_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HDF2457942.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC545_003203"
     CDS             complement(6034..7305)
                     /gene="serS"
                     /locus_tag="PC545_003203"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDF2457943.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEREFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKKIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYTLM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIK"
     misc_binding    complement(7431..7636)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7764..8645)
                     /locus_tag="PC545_003204"
     CDS             complement(7764..8645)
                     /locus_tag="PC545_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDF2457944.1"
                     /translation="MNFLSLAKTASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDVVKSILNEIFTNEKR
                     ILNDPKPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENDIKVPFVP
                     SSLLFNNTGNLNTMDKR"
     gene            complement(8657..9166)
                     /locus_tag="PC545_003205"
     CDS             complement(8657..9166)
                     /locus_tag="PC545_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDF2457945.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIALI
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
     gene            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC545_003206"
     rRNA            complement(9292..9408)
                     /gene="rrf"
                     /locus_tag="PC545_003206"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3007 a         1655 c         1013 g         3776 t
ORIGIN      
        1 ctcagatact attgttatca aaatatctgg ctttatggtt tgtttcttaa tataaattaa
       61 cctactgttt aattttcaat gttcgtgttt atttcttttt ccttttttca aggacaagta
      121 atactatatc atctttatct attctagtca atataatttt ttactttttt taattttttt
      181 atttatcatg tattctttat ctttaatttt taaaacttaa tgctaagtaa acctgtttat
      241 ttcataaaac taatatatat tctatgctct attttccttt ttattctata tttaaatttt
      301 ttatttacaa gaatattttt aatataacat atttgataaa attaatttac atttctttca
      361 cttgtaattt ttgcaatctt gtattagatt aatattaaaa ataatttatc aataacctta
      421 ttcacaaaga cagtttttat atatcaaaat ataatataaa tactatgcta cattttagga
      481 ggattttatt atgagcaaaa aagtattaat aatgtctgct tccactggtg gtgggcataa
      541 tagggctgct ctagctatta aagaagaact tacttccaaa acacttgatg gagagccaat
      601 tgaatgtgaa ataatagata gtttgaaatt agttaataat acaatggata aaataatttc
      661 aagaggatac gaaaaatctg ctatatatac tcccaaagcc tatggaagtg tatatagatt
      721 atctgaaaca aatcttttgt caaaaaatga atttaaagat aatctactaa taacattcat
      781 ggctaaaaaa tttaaaaagc ttattagaag cgaaaaacca gacttgatta taggaacgca
      841 tccttttcca atgattgctc taagtacatt gaaaaaaaac tttaatttac ataataatga
      901 atcaaatgct tacacagagc atttttataa acattataca aataccatta atgttcctcc
      961 gcttatttct gtattaaccg attatactac tcattctaca tggatacaaa atgaaattga
     1021 ttactatatt gttggtcatg aatatgtaaa agaattattg gtgtttgatg gagttgaacc
     1081 gagtaaaatt agaacatttg gaatccctgt tgaaaaatct tttctctctc acagagataa
     1141 agatattgtt ctttctgagt tgaatttatc tcctgataaa ttaactgttt tacttatggg
     1201 gggtagcttt ggggctggaa atataaaaga gacacttgat gaattactgg atactgatag
     1261 agactttcaa atacttgtaa ttactggtaa gaatgaaagc ctaaaagaaa aaatcgaaaa
     1321 aaaacttatg tctcgttatc atgataaaaa tgtatgtgtc cttggataca ctgataagat
     1381 gaatgatatt ttagcttcta tagatgtatt agtaagcaaa cctggtggac ttactacaac
     1441 tgaggctctt ttaaaagatg tacctatgat agttccttat tatataccag gtcaggaaga
     1501 agagaatctt gatttcttat ctaattgtgg tgcagcactt agaactacaa aaaaatataa
     1561 cttgtcaata ttactaaaag ttttaataga tgaccctagc agattagaaa tgctaaaaag
     1621 aaatattaaa tcaataagaa agtcaaattc agcccaaaat atagctaatt taattttaaa
     1681 tataatttct ggttgataaa ataaataaaa tccctatagt atatatatta tctattaggg
     1741 attttttatt ttctaattat ttaatattaa attggctcat aaaagtcttc ttcataatca
     1801 tactgataat ttttataatt atttctaaat tcagcatttt tatttcgtac atttttttta
     1861 gtttttttct tattagaaaa acttatatcc tcaaaatcat catatttagg tttatctttt
     1921 gatttcattt ttttaactct tttttctttc attgcattat gttcttctaa gttcataatt
     1981 ggtttcatgt tgcaccccta tcaaaatttt taaatgattt atacaacaaa ttatacaata
     2041 cagaagaaat ttattcaata aatctaaatc aaaattaatt tgaatttaac tcaaaaaact
     2101 acaaaaatcg aggtattaac ctcgattttt atatttctct tctgccttct agtgcttttg
     2161 atattgtgac ctcatctgca tactctaaat cgcctccaac aggaagacca tgagctatcc
     2221 gagtgacttt gattcccatt ggttttacta acctagcaat atacatggct gtagcttcac
     2281 cttcaatagt aggatttgtt gccattatta tttctttgac ttcctgattg ccaagtcttt
     2341 ggataagttc ctttattttt aacatatctg gtccaattcc atccattgga gaaattactc
     2401 catttaatac atgatactgt cctttaaact ctctcgttct ctccatagct gctacatctc
     2461 ttggatcttc cacaacacat ataacacttg aatctctact tttatttgaa cacatgctac
     2521 atgggtctgt atctgttata ttacaacaaa ttgagcaata tcttatttct ctttttgcat
     2581 ctataatcgc tttagataaa gcttccacat cattagtatt catattaata atatgaaaag
     2641 ccaatctttg agcagtcttt cttcctactc caggaagctt tgaaaattcc tctataagtc
     2701 tcgttatggg acctgtataa acttgcataa ataccaccta ctagaataac ccaggtatat
     2761 tcatacctcc agttacctta ctcatttgac ttgcttgtat atcatcaata tttctcaatg
     2821 cttgatttac tgcacttaaa actaaatctt gtagcatttc tatatcatct ggatcaacta
     2881 cttctggctt gatagttata tctataactt cttttttacc attaactttt acagtaactg
     2941 ctcctccacc aactgaagct tctacttctt tactttctaa ctcttgttga gctttttgca
     3001 tattttcttg cattttttga gcctgcttta acaagttgtt catatttcca ccaccaggca
     3061 tcattccgcc tccaaaacct tttttagcca taaacactat tcctcctaaa cttattttca
     3121 ttttattatt atatcataat agtattttat tatttactct cattttcatc aatactatct
     3181 tttatttcta atatctcttc actcactatg ttctttaata tttcctcacc aatgtccttt
     3241 ttctctttaa cttcaagatt tatatcttta acttctgatg tcagtactat ttttacatta
     3301 aaacttctat ttaaaacatc tcttattgtt ttttctaaat aatttattgt atcttgagag
     3361 ctcagtctag tttttgcaaa atcaaattta tcatcaaata taacatacaa catattagaa
     3421 tatacattaa aactttttac ttcacttaag agtgcgtata ttggcatttt tttatctttt
     3481 ttaatttgtt taagtatatt cttccaagat gactctataa gtataacatc ctcatttttt
     3541 acatcttcat aaactacatt ttcttgtttt acatctattt ctctatcgac ctcatactct
     3601 ttttctttat tattcccaat tttttcagac ttaaagttcc ccagttcaat cattttttct
     3661 aagttctcaa ctctttttat aagagcttct ttactttcat caaacattgg ttgagcaatt
     3721 ttcattatgg ttatttccac taaaaccctt ggatttgaag atgactttat atcatcctga
     3781 gttattgata atatatttaa aatccttatt aaatcattta tatctatatt tttagactga
     3841 attttaagtt gttctatagt ttcttctggt aatgaaatta tttcatccaa ttctgatgaa
     3901 accttacata ccattaaatt tctaaaatga tcaattaagt catttataag atttcttata
     3961 tctttgcccc ataagacaaa ttcatttaat atctcaagag actttttagt gtcctgctct
     4021 acaatacatt gagacaattc aaataattgt tctatattta cgcttcccat taattcaaca
     4081 acatctttat aatctatctt actttctcca aaagacatac attggtctaa tatactaagt
     4141 gcatctctaa gagctccttg agaatttcta gctattaaat taagagcttt atcatctata
     4201 tcaattcctt catcttcaca aatctttttc attctaaggg tcatatcttt aactgtaact
     4261 cttttaaaat cgtatctttg gcatcttgat aaaatggttg caggtatttt gtgaggctct
     4321 gtagttgcta atataaaaat tacatatgat ggaggttcct ctaaggtctt taataatgca
     4381 ttaaaagctc cttgagacaa catatgaact tcatctatta tatatacttt atatttagca
     4441 ttggctggag aatatttcac actttctcta agctctctta tatcatctac actattattt
     4501 gaagctgcat ctatttctac aacatccatt gtattatctt ttaatacaga ttcgcatatc
     4561 tcacactcat tacacggttc ttcatttatt ttattcaaac agtttactgc tcttgaaaaa
     4621 atctttgcag tagatgtttt accagtacct cttgttccac aaaacaaata agcatgtcct
     4681 atattatcac tatatatttg attctttaaa gtcttaataa tatgatcttg accaataaca
     4741 tcttcaaatt tttgtggtct atatgctcta tataatgctt tatgcatctt tttacctctc
     4801 tttagcaaaa ttaataaaaa aatccctcat tatctaaggg attagtcttt tatatattaa
     4861 atgctgtaca ccatctatcg acttaattcc ataggcgtta ccaaaacagt tagctcaact
     4921 taggcacccc cacggcacac aaaagtgatc acttaccgct gcttccttcc ggatctgacg
     4981 gggttcatga gctttcattg cgcaggacct aagcatcaac accacttatt tagggcggcc
     5041 ctacagaaaa taagccttta gatggaattc aatcctgcta tagcggattg caggttacag
     5101 ggcaccgcta cctccccatc tagtacagca aaattacaac catatttaat tactttcatt
     5161 gtttctggcg gagagagtgg gatttgaacc cacgatacag ttttacccat atactcgctt
     5221 tccaggcgag cgccttcgac cactcgacca tctctcctta atctcttttt ctcaatgcct
     5281 taaaaaattc ttgcaatatg atcgagcatt cttcttgcaa aacatcaaaa gctactttta
     5341 cattattatt attaaaataa tctaatttaa actcatgctg tttttctata tatgaatttt
     5401 ttacatgtct tgtccctatt ataagctttt ttattctaga attaactatt gcgccactac
     5461 acattatgca aggttccatt gtaacatata aatcacaatc agtcaacctc caaccaccta
     5521 atttttctga tgcttgtttt atcgctaaaa tttctgcatg tgccgtagag tcttttaacg
     5581 tttctgtaag gttatgagct ctagctatta tttgattatc tttaactata atagcaccta
     5641 ttggtgtttc ttttttatta tatgctttat atgcttcttt taatgcttct ttcatataaa
     5701 atgaactttc cataattcct cccaaaactt ataacaaact tatcttatca taattcattt
     5761 tataattcaa cttaatttta ttttgtttat ttaattatta ctatatatag ctatattttt
     5821 tttaaattag ttctaaaata atttttagat attcatacaa gagtgtttta taaaaatatt
     5881 tagtgtttta tgatgaaaat tctgctgtgt aaattaacat aaaaaaacta tgaaattaat
     5941 atttataata atttcatagt ttttttattt attgtttact cataatatat tctcttcatc
     6001 aaagcatact ctacatatac aattaatata aatttatttt ataacatcaa cacccatata
     6061 aggtcttaat tcttctggaa ctactacaga accatctgcc tgctgataat tttctaatat
     6121 agcagccaaa catcttccta tcgctaatcc agaaccattt aatgtatgaa catattctgc
     6181 ttttgatttt ttatcccttt taaatcttat tcctgctctt cttgcttgga aatcttcaaa
     6241 atttgaacaa gaagatattt ctacatatct attgtagctt ggcatccaaa cttctaaatc
     6301 atatttaaat gcagctgtaa atcccaaatc tcctgtacat atttttacaa ctctatatgg
     6361 aagacctaac atttgtaaca tagtttcagc atcatgagtt aatgattcta attcattata
     6421 agaatcttct ggtttaacaa atttaactaa ttcaacttta ttaaattgat gttgtctaac
     6481 taaacctctt gtatctcttc ctgctgaacc tgcttcagat ctaaaacaag gagtatatgc
     6541 acagtattta attggaagct ctgcaacatc taatatttca ttagcatgta tattagtaac
     6601 aggaacttca gctgttggta ttaagaaata ctctaatcct tctattttaa acatatcttc
     6661 ttcaaattta ggaagttgac cagtccctat aaaactattt ctattggcca taaatggagg
     6721 taagacttcc gtatagccat gcttagctgt atgagtattt aaaaagaaat tcattaaaga
     6781 tctttctaat ctagctccta atcccctata taaagtaaac cttgacccag ttattttccc
     6841 tgctctttca aaatctaaaa tacctagacc tgtacctata tcccaatgcg ctttatgctc
     6901 aaaatcaaat ttagtaggtt ctccccaagt tcttatttga acattgtctt catctgtttc
     6961 accttgtggt acctctggat gaggaacatt tggtattctc attaatgtat attccatttt
     7021 tgcttctact tcttttactt tctcatcaat accttttatc ttatctgata actctttcaa
     7081 tcttgccttt tcttctgtta catcttttcc ttcttttatt aattgaggta tttttttaga
     7141 atttacattt aactcatttt tcataacttc aacttcttga agtatttctc ttcttttatt
     7201 atctaattcc actacagcat caaggtcaaa ttctctttca cctcttcttt ccatagcttt
     7261 tttgatatca tctagatttt ctcttattct tttaatatct aacatattaa ttcctcctaa
     7321 taattttgat aatataagtt ataattccaa cttaatttac aaacattata cttgtaaact
     7381 cttagtttaa acaaatttta aaaataaaaa aagacctccg tttaccctat aaaagggacg
     7441 aaaatctttt atccgcgttg ccaccctaat tgactatact aaaatgtata atcctctcag
     7501 acctataacg taggtaaacg tctagcttta actagaagct ctagagtaga ttcaaaaacc
     7561 attgtaatga gttttcacca tccactcatt ctctgaatac aatctgtttt ttactatttt
     7621 ccttcacagc tttttaacta attgttttat ttgataatca ttatattata tatattaaca
     7681 tctttcaata ctattattca aattttttta gaattttctt taatttttta tattaaattc
     7741 attttttcta tttaattaag accttatctt ttatccattg tatttagatt acctgtatta
     7801 ttaaataata atgaagaagg aacaaatgga acttttatat cattctcatc aaatttattt
     7861 tttacctttt ccattacatc aaagtataca tcccagtaat catcagtttt tacccaaggt
     7921 ctagcaacta tttgcatggt gttatttcct atagagttta gacctattat aggctttgga
     7981 tcatttaata ttcttttttc gtttgtaaaa atttcattta atatagactt aactacatct
     8041 atatcactat catatgaaat attatatgaa aaatctattc ttcttttgtt ttgatgtgtg
     8101 taattaatta tattattact tgtcagttga aaattaggta taattatagt tttattatct
     8161 ggcgtcttaa gaacagtgct aaatatttgt atgtcagaaa ctgtaccttc tacacctgaa
     8221 ccttgaatat aatcacctat attaaatggc ttaaaaaata aaataatcat accagagcct
     8281 aaatttgata atatttcttt aaaagctaaa cctatactaa atccagcagc tcctaacaca
     8341 gctacaactg atgtagtttg tatccctata atacctaagc ttatcaatga tataatagat
     8401 aacatagata tatatattcc atatacaata aaattattta cacctttact cattccatat
     8461 ttgattagaa actttgaaac catccttctt actattttag atatataaat acctatcaca
     8521 aatacaatta ttgcatatat aatatttggt atattatcac taaaattatc gagcatattt
     8581 tctaaagttc tcttagttat attgctattt gttgaggcag tcttagccaa tgataaaaaa
     8641 ttcattcaac tcctccttat gataatattc cgttgtcctt taactctttt actatagctt
     8701 ctatagtttc ttcttttatt gcctcaattg tatgaatatg gctacattct gtcagaaccg
     8761 ataattgttt aaattcattt gtctcaactt tcttcataaa gttgtctata tccattttag
     8821 aataaatatt aagttctgca gttatctgac catatgttgg atgatttaca ataacatctt
     8881 ttataatacc acctaaatct acaattatct ttaattcatc ataaatttct ttagaatcct
     8941 tatgattttt acactttatt tgttttgtaa aatacggttg attatatatt atatatcctt
     9001 gaggtgtagc tattatttca aatcctctag ctcttataag ggctatatct tgaactatta
     9061 cttgcctact tacatttaat agttcagcta gtgttccacc ttttacagga tgcttagatt
     9121 cttttaaaat atctattaat ttacttcttc tttcattaga attcatatta caccttcttt
     9181 ccaaataaaa actttaccta tactatataa ttttatattt tatcttatta taaatcaata
     9241 ttgactttat attctattta atcttaactt ttataataaa taaaaaagac tacgtggcta
     9301 cgtcctactc tcccaggcag tttcccacca agtaccatca gcgctagaga gcttaacttc
     9361 tgtgttcgga atgggaacag gtgtatcctc tctgctattg taaccacata atctttatgc
     9421 ttaatatttc tattaagttt ttaataagtt t
//
LOCUS       DAMJVI010000125         9426 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.130, whole genome shotgun sequence.
ACCESSION   DAMJVI010000125 DAMJVI010000000
VERSION     DAMJVI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.130"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(123..776)
                     /locus_tag="PC545_003207"
     CDS             complement(123..776)
                     /locus_tag="PC545_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2457946.1"
                     /translation="MKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIGVSRTP
                     AREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQNLTESD
                     LVKMEELTEMMAVSIKYKNYNSYLKLSNEFHDIYITKSDNQVLVNLLNSLRYNFMSKS
                     YTSDNEDELYKMLTYSNNQHIEVVKSMRENDLENVERILREHWKTIPIAEMKKEKLL"
     gene            complement(1021..2886)
                     /locus_tag="PC545_003208"
     CDS             complement(1021..2886)
                     /locus_tag="PC545_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HDF2457947.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELTICVKVNEGYNIKSISL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDTPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELIDKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDN
                     DSVINKWVNMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFRSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYRDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRMLILVN
                     RSENVESISLCIESSFIEEIPIKYSLKNANSTIQIENNELKVDLDSKSFRIFRVN"
     gene            complement(2891..5311)
                     /locus_tag="PC545_003209"
     CDS             complement(2891..5311)
                     /locus_tag="PC545_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HDF2457948.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLNIEEEVREGLSELGINLDDLIE
                     TEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFVNGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRIKRMSIIDCDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKKDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNVDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC545_003210"
     CDS             complement(5325..6767)
                     /gene="glgA"
                     /locus_tag="PC545_003210"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDF2457949.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTDAVLEAISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQKYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDSSIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC545_003211"
     CDS             complement(6796..7926)
                     /gene="glgD"
                     /locus_tag="PC545_003211"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HDF2457950.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(7928..9088)
                     /locus_tag="PC545_003212"
     CDS             complement(7928..9088)
                     /locus_tag="PC545_003212"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDF2457951.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
BASE COUNT         2980 a         1718 c          918 g         3810 t
ORIGIN      
        1 atacattatt ctatcgtagt gccttagagt ttttatttgt atttaaagtt aaatttattc
       61 ttgtttttcc tttaatattt aatagtaaaa caacaaaatt ataaaaaaaa taaagagaaa
      121 ctttataaaa gcttctcttt tttcatctct gctataggta tcgttttcca atgttctctc
      181 aaaattcttt caacattttc taaatcattc tccctcatag attttactac ttcaatatgt
      241 tgattattgc tgtatgtcaa cattttatat aactcatctt cattatcact agtatatgac
      301 tttgacataa aattatatct taaagagttc aatagattta ctaacacctg attatctgat
      361 ttagttatgt atatatcgtg aaattcatta cttaatttta aatagctatt gtaattttta
      421 tactttatag agaccgccat catttctgtt aattcttcca tttttactag gtctgattca
      481 gttaaatttt gtaatgctga agaaccggct aacgcatcca atactccaat tacggaatag
      541 gtgtctaatt tctccttttc ctgaaattct tttactacaa atcccttatt aggtattttt
      601 tctagtaaat tttcacctgc taacctagtt aatgcttctc tcgctggtgt tctactaact
      661 ccgatagatt tacaaatctc agattcagta attctctgat tagcttcata ttctccattt
      721 tggattttct ttgatatata gtcatatact tgagttgtaa gtggatctac cttcataaga
      781 cgttcctcca tattaagatt ataaaagtta aatctcccta caactttggt agagagttca
      841 gttttatctt actaatttat ataatatcac aattatgata ttttttatac ttctttgtaa
      901 tattttgaaa tactaaaatt cttatgataa tttatacaaa aaaagtagaa aaagttttga
      961 taaaacaaaa ctctttccac attcgcctta ttttctattt atttttctaa aaaaagatat
     1021 ttaatttact ctaaaaattc taaatgattt agagtctaaa tctactttca actcattatt
     1081 ttcaatttgt atagtactat ttgcattttt aagactatac tttataggta tttcctcaat
     1141 aaaactactc tctatacata atgatatact ttccacattt tcacttctat ttactaaaat
     1201 taacattcta tcattatttt cattaactct aataaaagaa aatacagaat cattattagt
     1261 atatataaat tctgtatttc cagaacttaa aagtttattc ttatttctaa tttttataac
     1321 atctctataa aaattaagca tatcttcgtc ttcatttttc catggataag ttcttctatt
     1381 atctggatca acacctccac aaagacctgc ttcatctcca taatatatat atggtactcc
     1441 ttcaaatgtc atctgtatag ctacagcaag cttcactaaa tctacatcat catttaattc
     1501 cgttttagct cttgtaacat catgtgttcc aattaaattt aaattagacc taaatgattc
     1561 ttttggataa ttttctttaa tctgcatata tctattacaa agttcatatg aattaatttc
     1621 tccttttaag aaagaaaaca tgttatttct aaaaggataa cccataacag aatctaattc
     1681 ttcacctaat aaataacttc ttctctgacc atagctaatc ttatttgatg catcttccca
     1741 tacttcacct ataagaattg agtcatcatc tgcctttttt aattcctttt ttaactctct
     1801 tataaactta gttggtaatt cgtctgcaac atctagtctc cagcccttaa tacccatatt
     1861 tacccattta tttataacgc tatcattgtc atatattata taatccatat agctatgttc
     1921 taattcattt atatttggca atgtcttaac atcccaccaa cttttatatt tttgaggaaa
     1981 ttcttcaaac atataccatg aataatatgg agattcttta gattgatatg ctcctaaact
     2041 attatagttt ccatacatat taaaatattt actatctgct ccagtatgac taaaaactcc
     2101 atctaatact attgatatac ctttttcttt tgctttatca ataagttctt taaatgtatc
     2161 ttcatctcca aacattgggt caattttttt atagtcacct gtatcatact tatgattact
     2221 agaggcttca aatatagggc ttagatatag tatacttaca cctaattttt ttaaatatcc
     2281 aagtttattg attatccctc ttaaatttcc accatggaaa tcccatctta taacatcccc
     2341 ttgactatcc tttatgtaca taggtgtatc ttcccaattt ccatatatga aactattttt
     2401 tttaggatta tcaaccttac cactacgatt tccattattg aatctatcta caaatatatg
     2461 ataaagtatt ccttctttgt accatgttgg tactttaaaa tctttataaa cagtgatttg
     2521 atatttatta atatcactat aattatattc acaagaatat ccattatctc tgttttttcc
     2581 ataaaataaa gtctttattt gaccatccat ttcaacatca actttaaagt aataaaaata
     2641 aacacctgta ccatcaaact tttcaatctt accacaaaag cactttccaa gtttatcatt
     2701 atctaattct tcatttaaag ttattgttct catttcttct tctctattta tttctaggga
     2761 aatagacttg atattgtatc cctcatttac tttaacacaa atggtcaatt cctcatcaaa
     2821 ttttaaagct ccaaacggag ccttaaaatt tttatcccaa gagttatatt ctattacact
     2881 ttgcatttaa tcacaccctt ttaaaccaaa tgtcttttgc atactcagaa attgttctat
     2941 cagaagaaaa aaatccagag ttagctatat taactaaaga cattttattc catttttctt
     3001 tatctctata taacctgttt atatctgctt gagcttgacc ataattttca aaatctctca
     3061 atacaaaata ctcatcattg taagtagtga gtgaattata aatacttctt ccttcttcgc
     3121 caagattagg aataaatcca tttattaaat catctactac cctttttata tccatattag
     3181 aatgatatag gtcaagtgaa gaatatccac catatttatt ataatttagt acttgttctg
     3241 cactaagtcc aaataaaaac atattttctt taccaacctg ttcacatatc tctacatttg
     3301 caccgtcaag tgtagccaaa gttatagcac cattcatcat aaacttcata ttacttgttc
     3361 cagatgcttc cttagttgta gttgaaattt gctcactaac atttgctgct ggtattatta
     3421 tttctgctaa tgatactcca taattctcta aaaatactac ttttaacttg tcctttactc
     3481 taacatcatt atttatagta cttgcaacag aatttataaa tttaattata catttagcca
     3541 aataatatcc tggtgctgct tttgcgccaa atataaaagt tcttgggtca agattcaaat
     3601 taggattatc aagtaattca tgatacatat gaagtacatt aaatatattc agtaactgtc
     3661 ttttatacgc atgcaatctc ttaacttgaa catcaaatat cgaacttggg tctacattta
     3721 aatcgtattt atcttttata aactttgcta gatttgcttt attattgtat tttatatcat
     3781 caagtctttg taaaacagag gaatcatttt taaatttttc aatatttttt aattgtaatg
     3841 tatctttctt ccatgaatca ccaattaaat ctgtaattaa attgctaagt tgaggatttg
     3901 aacttataag ccatcgccta tgtgcaattc cattagtctt attattaaat ttatttggtt
     3961 catcttgata gaaatctttt aatacctctg tttcaagaat atgagtatga agcttagcaa
     4021 ctccatttac actatgactt gtgacaatac ataagtttgc catattgata ttgtcacagt
     4081 ctattatact cattcttttt attctatctt ggtcatatcc tttattattt aattcctcta
     4141 catatcttct atttatttct tctattatca tataaattct tggtaatagc tctttcatca
     4201 tacttacagg ccatttttcc attgcttctg acattatagt atgattagta taggacactg
     4261 tttttttagt tatttcccat gcttcatccc aagataaatt ttcttcatca agcaaaattc
     4321 tcataagttc tggtatgcat aaggtaggat gtgtatcatt tatatgaatt gctaccttat
     4381 cgaataaatt attaatattt aatttgttct ttttatattt tcttattata tcttgtatcc
     4441 ctgcacttac aaaaaagtat tcttgtttca acctcaacaa cttacctgca tagtttgaat
     4501 catcaggata taatacctgt gatatttcct cagtataata cttatgtttt aaagcctctt
     4561 catagctacc agaataattt agagcatttg aggtaagttc accaaaatct ctctttggta
     4621 tttcactctt aaacaatctt aaagtattta tacattggtt ttggtatccg attattggta
     4681 tatcataagg cattgccatt acaggaaggt agtttttatg aattactttt aaatgactac
     4741 cttccttgat taattctacc tcaccaccaa acttcaccat tgttgcttca tttggtctta
     4801 cagtttccca tgcatatcgt ccttctgtaa gccagttatc aggcacttct acctgataac
     4861 catttacaaa tttttgttca aacagaccat atttatacct tattccatat ccttgaccac
     4921 ttatgttcaa agatgccata gaatctaaaa agcaagctgc taatctacca agtccaccat
     4981 ttcctaaggc tgggtctact tcagtttcaa ttaaatcatc caagttgatt cctaattcag
     5041 ataaaccttc tctaacttct tcttcaatat ttaaatttaa taaattagat tttaactgtc
     5101 tccctattaa aaattctaaa ctaaaataat aaacttcttt tttcttatct aactttgtag
     5161 caacccattt ttttgctatc tcatctttca atagactaca taatacattt aacaattgtt
     5221 catcagtagc ttccttgata gattgtgcat ataagctata catttttact tcaaattttt
     5281 gcttgaattt ctttttgctt attgttacca aaaaaaccac tcctttaatc acatatgtct
     5341 ctatatgttt ctatatactc cttagctgat tttttccaac tattatctgt tttcattgca
     5401 ttttcaacta atttaatcca tttttctttg tccttaaata ctttgattgc atttttcaaa
     5461 cagaaaaaca tctcatgagc attgtaattt ttaaaactaa atccattgcc ttctccagta
     5521 aatttattat aaggtgttac tgtatctcta agccctccag tctctctaac tattggtaaa
     5581 gaaccatatc tcatagctaa catttgacct ataccacaag gctcaaataa tgatggcatt
     5641 aagaacatat cacttgcagc atatatctgt tgagccaatg atgcattaaa agtaattcta
     5701 gctgatactt tatctgaaaa attagaatcg taatagttga acattgattg atattgatgt
     5761 tctccagttc ctaaaacgac aatctgtaaa tcttctctca taagttctgg catcatgtat
     5821 gctattaaat caaatccttt ttgagaaact aatctcgaaa ctatacctat caacggaata
     5881 gatgaatctt ttttaagtcc caatatatct tgtaatctca atttattttc tacttttttg
     5941 tcaattgagt ctacatcata atgaacaaat atatctttat cttttgcagg gtcatttaaa
     6001 tcataatcta taccatttaa tatacctttt aattttcctg actcttttct aagtaatcca
     6061 tctaattgtt caccataaaa agatgtttgt atttcatttg cataagtagg actaacagtt
     6121 ataattttat ctgcaaaatt tataccagct ttcataaaat ttattgagcc ataatactct
     6181 atatctccat tatcaaagta tttttgtggt aagtccaata tatcatataa aacatgctta
     6241 tcaaatacac cttgatattg gagattatgt atagtataca tagtttttat atttttatac
     6301 ccctcaaggg ttgcatatct ttcttttaga attaatggta acatccctgt atgccaatca
     6361 tttatatgta ttacatctgg ataaaaatca attctactta tagcttctaa aactgcatct
     6421 gtaaagaaag tatatctttc tgcatcatct ccatatccat agatactctt tctttcatct
     6481 tctcttctaa aataaaattc attatctata aagtaaaatt ttacaccatc taattccatt
     6541 tctaaaagac cacagtattg atttctccat ccaacacgta cactaaacac agctatttct
     6601 tttagttgat cttttaaata acttggtata gttgaatatt tgggcattat tacccttaca
     6661 tctacacctt ctttttttaa ttctttaggc aatgcataag aaacatctcc taatcctcca
     6721 gtttttgcaa agggccaaca ctctgctgtt acataaaaaa ccttcattag tcattccccc
     6781 ttctaaaaaa tttatttata tatttgtatt cttttccact acaagaggat atttttcatc
     6841 tcctcttaat tctttcccat ttgatacact cgtattttta tctaatattg cattatatag
     6901 ttttgcattt gactttatta cacaattttg cattactatt gagttctcta taactgttcc
     6961 tttttcaaca tttacctttc taaatatgat actatctttt acagtacctt ctattaaaca
     7021 accagatgct ataaagctat tttctacttt agcactatct gtataaattg taggtgattc
     7081 attcttttct tttgtaaata tctttctttc agaatataat aattcatctg caacttctaa
     7141 atttaataaa tctttgctca tttcaaaata tgattcaaca gaatttacac attttagata
     7201 tcctgtatat tcataggctc ctattttcat attttctgct tcttcaataa taaattcttc
     7261 taaataacaa taattgcctt tatttacaat gttatatata cattcaacaa aatcttcacg
     7321 cttcataata tatgtctcca tacatatgtt tgctctaggg aacttaccta aattatttcc
     7381 tacatttatt accctattgt tactatctaa atttagagtt aagttaccag caaaatcttt
     7441 atttgcatta tttacatttt tatatattat agttatatca ttatgagatt ttttatggta
     7501 ttccaatgct tgagaataat ttaaattgca tagcatatag cttggagcta ttaaaatata
     7561 ttcttcttca ctcttttcta tataatctag attagctaag atagtatata tatcaccttc
     7621 tctatgtgag taagtatttc tatacttagt gttttctgga ctaaaaattg ataggccacc
     7681 tttttttcta cttaaatccc aatctttacc actacctata tggtctgtta atgatctgta
     7741 tttttctttt gcgaatattc caactttagt gattcctgaa tttatcatat tagataaagc
     7801 aaagtctatt attctatatc ttccagctat tggtgtagat gctattggtc ttccatagtt
     7861 aagtttgttt attgctgggt ctcctttttt gtttaaattt attatcccta aacattcatt
     7921 tctcatctta aagacctcct tctattaata cgcatctagg ttcgatattt tcatactcag
     7981 aaactacatt tatttcatta tttacattct ttatctgaac attatcattt atagttgcac
     8041 actcacctat catagctttt tctatagtta cattttcacc tattacaaca tttggcatta
     8101 ttactgaatc aattacttta cttcctttac caacaacaac tccatgtgat aatacagagt
     8161 tacctacttc acctaaaatt ctacaaccat cagctatcat tgatctatga acatttgcat
     8221 ttttacctat atattgtgga ggcattgcca tagtatttgt atatatcttc cacttagggt
     8281 ctgctaaatc aagtgtttct ggagatttta ttatatccat attagcatcc catagacttt
     8341 gtatagttcc tacatctctc cagtatcctt taaatggata agcatacatt ccaacattat
     8401 cttctaacat tttaggtatt aaattttttc caaaatcatc ataatttatt tctggatttt
     8461 tttctgcttc cttaaaataa tttctcaaca ttttccaatc aaatatgtat actcccatag
     8521 atgccaaatt gctcttaggt tcacttggtt tttcttcaaa ttcatagata cttgaatctt
     8581 catttgtatt cattatccca aatcttgaag cttcatccca aggaacttca atcactgcta
     8641 ttgtagcttt agagcctttt tccttatgaa attttaacat tttgctataa tccatcttgt
     8701 atatatggtc accagataat atcaatacat attctggatt ataggtatct acaaagtcca
     8761 tattttgata tatcgcatgt gctgtaccat tataccaatt tccttctttt tcattcataa
     8821 atggttgtaa aacatagaca ccaccattta ttctgtccaa atcccaatga cttcccatgc
     8881 ctatatgtga atttaatatc aacggtctat attgagttaa aacacctaca gtatcaatac
     8941 cagagtttga acaattgctt aacacaaaat ctataattct atacttacct ccaaatgata
     9001 cagctggttt ggcaattctt tttgtaaaaa cacctaatct tgaaccttgc cctcctgcca
     9061 aaatcatagc taacatctct tttttcattt aatgacactc ccctcatcta gtaattaata
     9121 tttataattg catttttact tttataaact ttatcagtca ataaatgtcg tgtataaatg
     9181 ttgcttaaat aataccttaa taaaaataaa ataatatcaa ctatacaatt gatataatgt
     9241 ataccccata atggacttac cgacgtatac attattttcc ataagcacta tagtatttat
     9301 taatattcaa ttattggtta aaaaataata caaattttat tttataaata taaaaacatt
     9361 ttttataaat cctttgtgtc atatgaacat gcttattttt taatttgtta aattcgtaat
     9421 agcaaa
//
LOCUS       DAMJVI010000126         9229 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.131, whole genome shotgun sequence.
ACCESSION   DAMJVI010000126 DAMJVI010000000
VERSION     DAMJVI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.131"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            268..1644
                     /locus_tag="PC545_003213"
     CDS             268..1644
                     /locus_tag="PC545_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDF2457952.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSKGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVRKRRFSKLNVHSKVVLFSTATLIFVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPSTIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1931..2602
                     /locus_tag="PC545_003214"
     CDS             1931..2602
                     /locus_tag="PC545_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDF2457953.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2791..3588
                     /locus_tag="PC545_003215"
     CDS             2791..3588
                     /locus_tag="PC545_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDF2457954.1"
                     /translation="MITNINSKDNEKLKYTRALLKSKNRNKESKFIIEGYRVVMLALE
                     CMANLDYVFINEEFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVDAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIIDASQDETVDMLKSLDFNIVSSYLNTENFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKYLI"
     misc_binding    3764..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4048..5067
                     /gene="pheS"
                     /locus_tag="PC545_003216"
     CDS             4048..5067
                     /gene="pheS"
                     /locus_tag="PC545_003216"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDF2457955.1"
                     /translation="MQEKLLALREAALAEIKEAQSIESVESLRVKYLGKKGEITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            5084..7477
                     /locus_tag="PC545_003217"
     CDS             5084..7477
                     /locus_tag="PC545_003217"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDF2457956.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     KDALIDFELTSNRPDCKCMMGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRNAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITSN
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVANDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNAKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFALKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEDVAKVHDKILSELSEKLNANLRSN"
     gene            7502..8092
                     /gene="zapA"
                     /locus_tag="PC545_003218"
     CDS             7502..8092
                     /gene="zapA"
                     /locus_tag="PC545_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDF2457957.1"
                     /translation="MNKVMVKIHGAEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAETKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDAKNVE"
     gene            8312..8995
                     /locus_tag="PC545_003219"
     CDS             8312..8995
                     /locus_tag="PC545_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDF2457958.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKTKIHWRQVIIIIEMI
                     TILIVSFIPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFISGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         3575 a         1010 c         1761 g         2883 t
ORIGIN      
        1 aaataaattt taagtacaaa atagtaaaag ctatgatagt tttagaccta ttatatgtaa
       61 aaaatataga tgtttttggt gcaatttaaa ttaaaagata tatataaaaa tttaaaatat
      121 tccaaacaga aattataaaa attactatca ttgtctttat acaatattta tactataata
      181 gaataataat ttagaggaaa gctctaaaaa taaacaaatt aagggtctta acagtagtga
      241 ttttatgtta aggaggtata aagaaagttg aaaacaatag ttagaagact tattctctac
      301 gcagatggaa tgcgtcctac tcaaataatg gtatctgggt ttgctgcgat tattgtaatc
      361 ggagcattgt tgttaacact cccgatagcg tcgcagagtg gagagagtat aggcttatta
      421 aatgctctat ttacggctac ttcagcggtt tgtgttactg gtctagttat ggtagataca
      481 gcaacttatt ggagtttatt tggacagata gttataataa cattgataca aattggtggt
      541 cttggattta tgaccgtagc cacaatgttt tcacttatgg cacgaaaaaa aatacaacta
      601 agagaaagat tattaataca agagtcatta aatcaagctg atttatcggg acttgtaaga
      661 cttacaagat ttgtattaat aataaccata acaatagagg gaataggtgc actggtttta
      721 tcaactgtct ttattccaca atttggtttg tctaaaggga tatggtatag tgtatttcac
      781 gcaatatcag cgttttgtaa tgccggattt gacttgatgg gcagtgtaag tggcccattt
      841 acatcgttaa attcttatgt aaacaacttt acagtatcaa tgactgtttg cgcacttata
      901 gtgcttggag ggctaggatt ccctgttgta cttgatattg taagaaaaag aagattttca
      961 aaattaaatg tgcattctaa ggttgtattg ttttcaactg ctacacttat ttttgtgggt
     1021 gcattattta tttttcttat agaatttaat caaaaagcta ctatggcgga cttgccttta
     1081 aagggaaaag tactatcagc aatatttcaa tcagtaacag caagaactgc tggttttaat
     1141 acgcttgatt tagctacttt acgagaaagt agtgtatttg taatgataat tttgatgttt
     1201 attggagcat cacctgcatc aactggtggg ggtataaaaa caacaacact agctgtttta
     1261 atcattacag ttagaagttt tctatcagga aaatcagata tagaagcttt tgaaagaaga
     1321 ttagctccat caactataaa aaaatctctt ggtatatttg taatcagtat atcagcagtt
     1381 atttttggaa cattgattat ttcaataact cagcctaatt ttacattagt tcaatcagct
     1441 tttgaggtta catcagcact tgctacagtt ggttcaagtt tagctggaac tccaaactta
     1501 aatgctttag gtaagattat aataataatc tttatgttta tgggtagagt tggttctctt
     1561 actttattta tggctatact ttctggtggt agaagaaaga gtcaaccaat cagatatgca
     1621 gaaggtaaaa ttatggttgg ttaagttacc aagaagtagt ataaatttat aatggattta
     1681 gagtagcact gtaaatctgt tgataaaata aatgagccta gatgtaatta tatgaagaaa
     1741 taaaatcgac taatgaacaa aattaattaa tgaaataaaa aaacagggaa ataaaataca
     1801 aataaaaagg taaatcaaaa ttaaattaat ctgtgtatat aaaattatat attgtgtata
     1861 agttttgtat gtgtaaacaa ttaatttaag agatattata taaattgtgt attagggagg
     1921 aagtttcgag atgaagcaat atatagtcat tgggtgtggg agatttggaa gttcagttgc
     1981 gtctactatg catcttttag gacatcaagt aatggcaata gacaaaaatg aagattcagt
     2041 tcaaagtata tctgacaagg taacccattc acttatagtg gatgttactg atgagcaagc
     2101 gttaaggtca ttaggtttag gtaactttga tgtagcagta gttgcaatag gttctgatat
     2161 aagggcatct ataatggcga ctcttatagc caaagaaatg ggtgtagagt tgataatatg
     2221 taaggcaaag gatgaattac aagctaaagt tctttataaa attggtgcag atagagttgt
     2281 atttccagaa agagatatgg gagtaagagt tgcacacaat ttagtttcgg ataatatatt
     2341 agaccatatt gaacttgacc cagagtattc aattgttgaa atcgtaactc caaatagttg
     2401 ggttggcaag acacttatag agcttgaatt aagagctaga tatgagataa ctgtacttgc
     2461 tataaaaaca ggtaaaaata taaatgttac accttctcca gatgaggaac ttacagccgg
     2521 aagtatccta gttataatcg gtcaaaatac tagtataaca gcgataacat ctggaaataa
     2581 ggggataatt agaagaagat aatttactat ttaatatata tttaattgca atgaaagtag
     2641 atagtatcat ataattatga atagttatat gatactattt ttttaatcga aggtagtatt
     2701 tatttgtaag attagataaa gagaaattaa attaaaagta aggaggctgt gctaatataa
     2761 aaatttataa gttattagca tttagataaa atgattacaa atataaacag taaggataat
     2821 gaaaagttaa agtatacaag agcactatta aaatcaaaga ataggaataa agagtcaaag
     2881 ttcataatag aaggatacag agtagtaatg cttgcacttg aatgtatggc aaaccttgat
     2941 tatgtattta tcaatgaaga atttgaaaat aagaaagaac atgtaaaact attagaagat
     3001 ttggataaaa aaaacacaaa gatatacaag actactaata aaaactttaa agaattagtg
     3061 gatacagaaa atactcaagg aataataggt gtagtttcat ttaagaaaaa aaaattaagt
     3121 gaaagtataa ataaaaaaga taaatttgta ttgattttag acagaataca agacccagga
     3181 aatatgggga ctataataag gactgctgat tctgctggag tagatgccat aatagcacta
     3241 aagggatgtg tcgatatata caacccaaaa gtaattaggt ctactatggg ttctattttt
     3301 gatatgaata taattgatgc ttcacaagat gaaactgtgg acatgcttaa atcattggat
     3361 tttaatatag tttcaagtta cttaaataca gaaaattttt atgacaaaat agattatggc
     3421 tcaaaagtag cattggtgat aggaaacgaa gcaaatggaa taaatgaaga acttgtatca
     3481 aagtctgata ttttggttaa gatacctata tatggtaaag ccgagtcgtt aaatgctgcg
     3541 ataagctctg ctatactgat gtatgaaata aaaaaatact taatttaatg tattgtaata
     3601 aatataatgt catgttataa tcttgaatta aatagtagag tatcaaaaat agtcaatata
     3661 tattaaaaaa ataattaaat taattatgta ttggatatat attaaaaata gtcacataat
     3721 ttgaatggaa atgatataat actaaaataa acaatataat attgtaaatg caatgaaaga
     3781 ggaaagtatt ttgattaaac ttagtaaaga gataaacacc taggctggga gtgttttcta
     3841 agaggtcatg agaagttccc tctggagtaa cagagctgaa attttacagt aggctttgac
     3901 gttaaaaacg cgttaagttt gttagaggtg gtttgatgat ttttaaattg ttaaactact
     3961 agggtggtac cgcgaaacta tagcctttcg tccctagaca gggattgagg ggcttttttt
     4021 atacaaaaaa acgaaagggt gatatgtgtg caagaaaaat tacttgcttt acgtgaagca
     4081 gctttggctg aaataaaaga agcacaaagc atagaaagtg tagaaagttt aagagttaag
     4141 tacttaggaa aaaaaggtga gataactgcc atacttaaag aaatgggtaa attatctgct
     4201 gaagaaagac cagtagttgg taaggttgcc aatgaggtaa gagaaaacat tgaacttagc
     4261 ataaattcca aaaaagaaga aataaatgct attgaaaaag aaagaaaatt aaaagaggaa
     4321 gtgatagatg ttactcaacc aggaaaagtt ttaaaggttg ggaagaagca tccaataact
     4381 caaattatag atgaagtaac agatatattt atcggaatgg gattctctat agcagaaggg
     4441 ccagaagttg agactgttga aaacaacttt gacgcattaa acgctcctaa agaccatcca
     4501 tcaagagata tgagcgatac attctatatc aatgatgggg tattacttag aactcaaaca
     4561 tctccagttc aagtaagaac tatgagaagt caagagttac caataaaagt aattgcacca
     4621 ggtagatgtt ttaggtcaga ctcgccagat gctacacact caccaatgtt ccatcagata
     4681 gaagggcttg ttgttggaaa agatgttact atggcagaat ttaaaggaac tatggatatc
     4741 ttcgttgaaa aattgtttgg ttctgatatc aaaactaagt ttagacctca caactttcca
     4801 tttacagaac caagtgcaga ggttgatgtt acttgtttca aatgtggtgg taaaggttgc
     4861 ccaatgtgta aatatgaagg ttggatagaa atattaggtt caggtatggt tcatccaaat
     4921 gtgcttagaa attgtggaat agacccagaa gtttacagtg gatttgcatt tggagttggg
     4981 gttgaaagac ttgcaatgct taaatacgaa atagatgata ttagattatt attcgaaaat
     5041 gatatgagat tcttaaatca attttaatta ggagggtatg tagatgttag tatctttaaa
     5101 atggcttaga gactatgttg atatagacat ggatgtaaaa gagttcgctg ataaaatgac
     5161 aatgacagga actaaagttg aaacaataga ttattatggt gaagaaatag aaaatatatt
     5221 ggttggaaag attttagaaa taaaacaaca tccaaatgct gataagttgg ttgtaaccaa
     5281 agtagatatt ggagataaag ttgttcaaat agttacagga gctacaaata tatcagaagg
     5341 agattatatt ccagtagctg taaatggttc taagttacct ggaggagttg aaatcaaaca
     5401 gactgatttc agaggtgaat tatcagatgg tatgatgtgt tcagcagctg aactaggtat
     5461 agatgaacat tacattgagg agtataaaag aggtggtata tatattttag accacgaaga
     5521 ttcttatgaa ttaggaaaag atataaaaga tgttttagga ttaaaagatg ctttaataga
     5581 ttttgaatta acttcaaaca gacctgattg taaatgcatg atgggtatag ctagagaagc
     5641 agctgcaact ataggaacaa aagtaaaata tcctgaaatc gaagtaaaag aaagtgacga
     5701 agagatagat ttcaaagttg agatagataa tccagattta tgtagaagat atgttgctag
     5761 aatggttaca gatgtaaaaa tagaaccttc tccatattgg atgcaaagaa gacttacaga
     5821 agcaggagta agacctataa gtaacatagt cgatataaca aacttcgtaa tgttagagct
     5881 tggtcaacca cttcatgctt ttgatataaa tcaagtagag actggaagaa tagtagtaag
     5941 aaatgctaaa gatggagaga aacttgtaac attagatgat gttgagagaa cattagacaa
     6001 agatatgcta gttataacaa atggagaaaa atcacttggt ttagctggtg taatgggtgg
     6061 tgctaactca gaaataactt ctaatacgaa gactgtactt tttgaaagtg ccaatttcaa
     6121 accagaaaac ataagaatga cagctaaaaa agttggtatt aggtcagaag catcttcaag
     6181 aaatgaaaaa gacttagacc ctaatcttgc agagatagca gcaaatagag ctgcacaact
     6241 tgttgaaatg ttaggagcag gaaaagtttt aaaaggtgtt gtagatgtat atccaaataa
     6301 accagaacct aaaaaattgg tagtaaatcc tcaaagaatt aaccacctat taggtgtaga
     6361 tgtaccaatg gagcagtttg taggaatttt agaatcatta gagtttaaat gtaatttggt
     6421 agctaatgat aaattagaaa tagatgtacc aagctttaga acagatatgg aacaagaagc
     6481 tgatgtatgg gaagaaatag ctagaattta tggatttgag aatatacctt ctgtacaatt
     6541 agaaggaaat acgacagcag gaattaaaac ttcaaaacaa aaattcatgg atgctttaaa
     6601 agataattca actgctgtag gattaaatga aatattaaca tattcatttg taagccctaa
     6661 gggagtagat aagataagag taccagaagg taatgcaaag agaaattttg ttaaattact
     6721 taacccatta ggagaagaaa cttctgtaat gagaactact ttaataccaa atatgttaga
     6781 tgttttatca actaatgtat ctcataaaat agaagaagta tctgcatttg agtgtggaca
     6841 tatattcatc ccacaagatt cagaattacc taaggaagaa aatagaatgt gtgtaggtat
     6901 gtatggtaaa gatgttgact tctttgcact aaaaggaact atagaaacta tacttgtaaa
     6961 tgtcggattt aaaggttatg aaattgaacc tcaagataat aatactacct tccaccctgg
     7021 tagatgtgct aaaatagtgt ataataataa atatgtaggt acattaggtg aattgcatcc
     7081 agatgttata gaaaactaca atctaggtca aagagtttat gtcgctgaaa tagatattga
     7141 ctttgtattt gataattctg atagaacaaa gaattatgtg ccattaccaa aatatccatc
     7201 tacatcaaga gatatagctc ttattgttaa agatgatgta tttgtaaaac aaatagaaga
     7261 tataataaag gaaaatggtc aaggattagt agaaagctac aagttatttg atgtttacaa
     7321 aggttctcaa atagaagctg gatacaagtc aattgcatat tcaataactt atagaagtaa
     7381 agataaaact ctaacagatg aagatgtagc taaggtgcat gataagatat taagtgaatt
     7441 aagcgaaaaa ttaaatgcta acttaagatc aaattaacaa ctaatgtcgg gggaggttag
     7501 gatgaacaaa gtaatggtta aaatccatgg cgctgaatat ccaatggttg gagacaaatc
     7561 agagaagttt atgattagta ttgctgattt tgtagacaaa gaaatggata aaataacacg
     7621 tcaaaacccg aaattgagct tatcagttgc tgcgatatta actgcattaa atatatcaga
     7681 ccttttattt gaatgttcag atgagaatga gaagttgatt aaagcgaatg aagaattgag
     7741 taagaaggtt ggagcatcta atgaagagct tcaattagaa atcaaatctt taaagcttac
     7801 gatagcagaa aaagaagcag aaaatcgtga agctgaaact aagatgaaag agttaattga
     7861 gattatagaa aataaaaagc aagaaatatt cgaacttagt aatacgacag aaggttctag
     7921 agcagagtta gatgcttata aaaacaagat tgaggagtta agtactcaac ttgaagaagc
     7981 aaatgaaaga gctactatag ctgaaaactt agcttctgag tttcaaaaca aagcttatga
     8041 tttacagtta aaatgtacag ggttaaataa tgatgcaaaa aatgtggaat aaactaaaag
     8101 atttgctgtc tcttgttttt tgagacagct tcttttaatg tataaatata aatgctatat
     8161 agatatagat aatgtttaaa tataaataaa tataaatgat gtataaatat agacaatatt
     8221 taaatataaa taattttttt atatcaatga aaaatagaat ctaaaatgat tttgattttg
     8281 tatctatcat tagaaagcga gaggtttata tatgacaagc aagagtcaga tgtctgaatc
     8341 attcttttta tgttctttgt tggcaataac aggaggattt ttggatattt atacttatgt
     8401 ggtaagaggt aaagtctttg ctaatgcaca aactggaaat atagttttgt ttgggcttaa
     8461 tatagcagaa ggtaatgtaa aggaatcatt ttattatttg attcctattt tggcatttat
     8521 gttaggtgta tttatagccg agaagataag aaagtatttt acaaataata agacaaagat
     8581 tcattggcgt caggttatca ttataattga aatgattaca atacttattg tttcatttat
     8641 tcctagagga agatttaata tgtttgtaaa tgttgctatt tcttttattt gttcaatgca
     8701 agtagagagt tttagaaaag taaatgggaa tagttttgca acgacaatgt gtactggaaa
     8761 tttaagaagt gctacagaaa ctttatttca ttatatacat actaagaata tttttatgat
     8821 aaaaaagagt ttacagtatt atgctattat tatattcttt atatctggag ctattttagg
     8881 aacttttttt acaaaaattt tcgtggaaaa atcaatacta atatgttttt ttattttggc
     8941 agtggtcttt ggaattatgt ttatcaataa agatgattca tttaatgaaa attaactttc
     9001 tgtaaattta caaaaaaatg aagctgttat ttgatatact ttatatatag caaatattgt
     9061 atagattaat taaagaattt aaaatatagt aagcagtgta taaattaatt aaaatgtctt
     9121 aagtatagta ggcaatatat agattaatag aagtatttca agtatagtaa acagtgtaca
     9181 aattaattaa aatgttttaa atttagtaag cagtatataa attaattga
//
LOCUS       DAMJVI010000127         8872 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.132, whole genome shotgun sequence.
ACCESSION   DAMJVI010000127 DAMJVI010000000
VERSION     DAMJVI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.132"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(35..643)
                     /locus_tag="PC545_003220"
     CDS             complement(35..643)
                     /locus_tag="PC545_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDF2457959.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISD
                     FYYDDKGKKVFSKDELDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(752..3490)
                     /locus_tag="PC545_003221"
     CDS             complement(752..3490)
                     /locus_tag="PC545_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HDF2457960.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNINLYTVSINMYRIILLW
                     IFGYSIVYISSFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFSDINWNMIIILINVCISMFNLLY
                     LLNKLDSNIEHYKLIKDLTYFVFFTTIICFVAISKINIVDSDRLIGVIYLLLCNHTYY
                     VYVYSLNNRVVYPYYELDSINKKLSKKSEQLGKINLAIEKDMIIQRTLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMNNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNDKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRNINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCVSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLIE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNAIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIDKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKVIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3638..4429)
                     /locus_tag="PC545_003222"
     CDS             complement(3638..4429)
                     /locus_tag="PC545_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2457961.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKSLDELKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIVIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4465..5121)
                     /locus_tag="PC545_003223"
     CDS             complement(4465..5121)
                     /locus_tag="PC545_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2457962.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSTINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALIILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5114..6079)
                     /locus_tag="PC545_003224"
     CDS             complement(5114..6079)
                     /locus_tag="PC545_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HDF2457963.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGSVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERKTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKNLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6185..6291)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6581..7525)
                     /locus_tag="PC545_003225"
     CDS             complement(6581..7525)
                     /locus_tag="PC545_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HDF2457964.1"
                     /translation="MSRAERLIELMITINAKRSFTAGELAEEFSVSKRTILRDLQVLE
                     SIGFPLYSKVGAAGGYHVLKERILPPIAFSESEVKSIFFAYQSLEYYNDLPFEQETIS
                     VLKKFLNCIPNDIQYNIENIRRKFVFWTPDRHCSTPLLKELFNIVMNESTIKIEYSSK
                     QKNSVRTIVPIGLYAMNGLWYCPAYCIKSASIKEFRVDRIVKILSIENLSSKKYKVLS
                     SIHDYLKNMEVGTDYHIKINLTDEGVKRCETEFLLARGLKILSKGGYIDMYIPKSTLN
                     WVAEYFLTFGKNATIIEPIELKHLVKSKVLELYNHHCI"
     gene            7636..8103
                     /locus_tag="PC545_003226"
     CDS             7636..8103
                     /locus_tag="PC545_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457965.1"
                     /translation="MENNTIKADDIINYCLSNLDDVLLMNSWGERAIYYNPNGILKRG
                     VYVLTIKEKDSNHDKGSLVSRPNVYRVNIGLKKETFIEMFGYIPKRPGVGQIVDMDFD
                     FTKLDTIMPHPIYSWMGWICVLSPTEKTFENFKTLIGESYNFAKQKFKKRKNK"
     gene            complement(8273..8872)
                     /locus_tag="PC545_003227"
     CDS             complement(8273..8872)
                     /locus_tag="PC545_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457966.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTKNNEKPSENTGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLIAEGERLVKDIDKRIEKLDKVSDEDMKKGQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
BASE COUNT         2874 a         1477 c          865 g         3656 t
ORIGIN      
        1 ttcctataga tacatatctt acctatataa aaacttattc tatataaaaa cgccataaaa
       61 aatcttttgc ttcttctgca taatctatat ttatacgttt actagttttt atatctaact
      121 catctttact aaatactttt tttcctttat catcataata aaaatcactt atatataatt
      181 cttcacccat tatactctta ctatttagac ttctatctat ttttaaagct ttgcaaagtt
      241 ttcctggtcc gttagtaata ttttttactt gatatttact caactcagtg taagctttgt
      301 tgtatctatt catagatatc tcatctaaag aagttatagg ctctacccct cttattaata
      361 cacactctgg cacattttct atatttgcag ataaattcaa gcaattatac ataccataaa
      421 ttaaataaac atatatatgt ccaccatcta aatataatgg cttagttcta tctgtctttt
      481 tattgccata tacatgtgct cctttatcat ttacacccat gtatgcttct gtctctatga
      541 ttttggtaac aattacttta ttttcatatt tccttattag atatttacct aatattgatt
      601 tggctaaatc aattccattt tttctaaaaa aatccttttc caaaatttca cctcataaaa
      661 tttatttaaa acgcatcaaa tacaactaaa gtaataaata aaattaactc ttattaatgt
      721 gatatatata aacaataaat ttagacattt attatttata tatatcaaaa aattctatct
      781 caaacttttc aatattatca ttgtaatttg aattatcata tacttcttca acaacctctt
      841 taattgggaa tataactttt acattagttc cttctcctac cttactatct atgtaaatct
      901 tcccattttg aatttcaatc aatgattttg taaggcataa acctatacca cttccaaatt
      961 catttgatat accactgttt tcaatttgtt taaatcgatt aaatatatca tttaattttt
     1021 ctttgtctat gcccactcca gtatctttta taattatctc tactgtttcg tcttttttat
     1081 atatacctac aaggatttct cctccttcta aagtaaattt tatagcattt gataatatat
     1141 ttagtattat tctttctatc gattctgggt caagccctac cataacagtt tcttcatctg
     1201 tatcaaaaat taaatctatt cctttagact tagcataact actaatagat gtaactattc
     1261 tttctgttat gtcaataata tcaaaattaa ctgctttaat cttatatatg tctgacttaa
     1321 cttgagaaat atttattata ttatctatta atctcaataa tctaaacata ttttgtttag
     1381 ttatttttat atgttctttt agtatatcct gaaaatcaat tatttttcct tttttataaa
     1441 ggttttcaat aagttgaact gaactatata tattatttaa aggtgtctta agttcatgag
     1501 atatattagc ataaaactcc atcttcattt ggtcatactg tctttgtttt attaatgctt
     1561 ttttaagtct ttcagactct ctttgcacac ttaaatcttt aataattctc actctacttc
     1621 ttttctgtcc aaagtataat gttcttgata tcatttttac ttctttaatg tgattatttt
     1681 catttactat atggatactc ttctctcttt ctcctatatt taaattgttt atgtattttt
     1741 ttactaatat atttctatat ttttgtgttg taactcctaa tacatcacta gaaattccga
     1801 acaactctct aaaggctcta tttgtatata gtactcttga tgtacttaaa tcctcaagaa
     1861 aaatagcctc tggtataaca tccagtaatt tttcatatat tattttttgt tcatttactg
     1921 ttctaagaat atttatctct ttagttatat ctctaaatgt aagcaattta aagagtttat
     1981 tatctgacca atagtctcca actctaactt cttcaacatg ctcttcacct tctgagtcag
     2041 acatcacaaa attaactata cagtttgctg tgtcattgta acttactatt tcagctttct
     2101 tttcttgtgt tagatttaat cttttaaatt ccctattgtc aaagaaatct atgttaaaag
     2161 tttcaaaata ttgttctaat gtcatatcga ctaaatcact tgaaacacat ttaaaactct
     2221 tttcatattt tttatttata cttattacct tattcttctt tatgccacct tcagcacttc
     2281 ttacaagtat aggagatgga atatttagta taatgttttc atagtcttta tttatatttc
     2341 ttatttcttc atctattttg atttcatttg taatatcatt taaacttatt aagtaattgc
     2401 tttcatcttt attattactt aaacttaaca aataaatttt atcatttaac tctaccagtt
     2461 tatcaattat acttatatcg ttatcaaatt tcttcaattt ttcagcaact ttttcatctt
     2521 cctttataaa agttaatatt ttataataat tattcatatc ctttgaaaat tcatctttaa
     2581 atttattatt tgtgtaactt atattaaaat catagtctgt tactatccat acatttggta
     2641 ttgtatccag agcttgtcta agtaattctt ttctctggtc tatgtagttt tttaatgttc
     2701 tttgtataat catatccttt tctattgcta aatttatctt tcctaattgc tctgactttt
     2761 tacttaattt tttattgatt gaatcaagtt cataatatgg atacactact ctattattaa
     2821 gactgtatac atatacataa taagtatggt tacataatag taaatatatt actcctatta
     2881 atctatctga atctactata ttaatcttag aaattgctac aaaacatata atagttgtaa
     2941 aaaaaacaaa atatgttaaa tcttttatta acttatagtg ctcaatgtta ctatcaagtt
     3001 tattcaacaa atatagtaaa ttaaacatag atatacaaac attaattaat ataataatca
     3061 tattccagtt aatatcacta aaaatatttc ttataaagta atctaatatc acaaaagcac
     3121 tataaaaaat tagtattggg cttattctta gtttttttac tttagaaatg gttaagtata
     3181 taaaagtaga catattaatt atgcttaagt taagcaatac aatttggaat ttttgaattt
     3241 ttaaatttac tactcctaaa atacttacaa aaactaatac aagacaccct actttcaata
     3301 tatttataaa ttcatctgtt ttaaagctac ttatataaac tatagaatat ccaaatatcc
     3361 aaagtaaaat tattctatac atatttatag aaactgtata caaatttata tttaaaagtg
     3421 caataaccaa aaataaccca ttagctagta tatagacctt attcttactt aaagttcctt
     3481 tagattccat attccccacc acttcttcat ttttatatag ttttacctat aaatatgtta
     3541 ccacaattct tccaaaaaat ttgtcacttt ctagttataa aaaaaaatga gtaaatattt
     3601 ttaattttac tcattttgta gattttgtat attcagttta aaaagctgga actatacttc
     3661 ctttatattc atcttgtata aattttttaa cttctttact attcatagcc tctgataaag
     3721 ccttaatctt atcactatct ttattatttt ctctacaagc tattatattt acatatggag
     3781 aatcacttga ttctattact attgcatcct tagtaggatt taagttagct gttaaagcat
     3841 aattagaatt tattactgct ccgtcaacat cttttaatac tgttggtaat tgctctgcat
     3901 tcatttcttt tattactata tttttaggat ttttagttat atctttttta gttataagct
     3961 ctccatcttt tacttctatc aatttatttt ttgctaataa tttaagtgct ctagctccat
     4021 tagttgcatc atttggcaca gctataacag ctccatcttt taattcatct aatgacttaa
     4081 ctttttctga atagaatccc attggctcta tatgaacttt agatgtataa gtaagtttat
     4141 atcctttctc cttcacagtt tcctctaaaa atggaatatg ttgaaagaag ttagcatcca
     4201 aagacccttc atctaaagct gtatttggaa gtacataatc atcaaatatt tttacttcta
     4261 aatcatatcc tttttctttt aacaaaggtt ttgcaacttc caatatcttt gcatgtggat
     4321 ttgatgaagc tcctactagt atttttttat cctctttgtt tgaacaacct actgctgata
     4381 ttgctattgc tgacaccaat gctactgata ataatttctt taatttcatt tttactcccc
     4441 ctctttaaaa tgtttataat taatttattt tagtttctta taagccagat ttcctactcc
     4501 ctggattatc tgaactaata ttataagtgc tattacagta tataccatta ctgctgtatc
     4561 aaatctttga tatccataaa taagtgctat attacctaat cctccagctc ctactgcacc
     4621 tgccatagct gtataaccaa gtatagatat tattgataat gtaattccag atactattga
     4681 aggcatagct tcctttatca taactttaaa tactatttgt ctttttgtag ctccaaaaga
     4741 ttttgctgct tctatcaatc ctttatcaac ttcatttaat gagctttcta taattctagc
     4801 gataaacggt gctgccccaa tagttattgg cactattgca gcagtttccc ctatacttgt
     4861 tccaacgatt aatctagtta ttggaatcat tgcaactata agtatcataa atgggaaact
     4921 tctaaatata tttactataa atcctaatgc actatttatt gtgctatttg gttttaatcc
     4981 atctggtttt gtaactacta atatgattgc taatataaat cctaatattg ttgccactat
     5041 agttggtact ataaccatat acaaagtttg taacaatgca tttggaaata gtgttgttaa
     5101 aaagtcaatt aaactattca tttatcattt cctcccatct tatgccctta ctagttagat
     5161 attgttttac ttgctctcca cttttatctg aaatgtttat aataagtgaa cctaaaatat
     5221 catctttaaa ttgttctaat ttcccaaata ttatagacac atctatattt agttctcttg
     5281 ccatagtcgt aataatagct tcattagaaa tatctttagg gaatagtatc tttatatttg
     5341 ttccttttgg caatattata ctttcttcac ctattaattt tctaagtcct ttagtatttc
     5401 ttaagaatat ttcttctgta tctcctacct ctaatacttc tccattttcc attattgcta
     5461 ctcttccaca aatctgcttt ataacttcca tttgatgagt tacaactatt attgttattc
     5521 ccaaattttt atttatatct tcaagcaatg aaagaattga ctttgtagta tttgggtcta
     5581 atgctgaagt tgcttcatca caaagaagta cttgtggatt tagtgctaaa gctctagcaa
     5641 ttgcaaccct ttgtttttgt ccgccactta gatttctggg tttatcattt ttcttttcag
     5701 atattccaac tacttctaat aactctaaaa ctcttttttt aatttctgcc tttgagtatc
     5761 caaaacattc taatggcaat gctacattat caaatacagt ttttctttca agcaatgaaa
     5821 agtgttgaaa aatcattcct aactcttttc ttaaatctct catttgcttt tcattcaatg
     5881 actttacttc tttatcagaa acaagtacac tgccttcttg gtattcttct aatccgttta
     5941 tacatctcag tagtgttgat ttccctgcac cactatgacc tattattcca aaaatttctc
     6001 cagattctat ttcaatactt acatctttaa gcacttgaat ctttccataa tacttattaa
     6061 cattttttat gctaatcata aaatttcctc ccatatattc tgctcagttt ttatgtttat
     6121 cctctcttat gtattacaac aaaataaaag cccgagtata aactcaggct tagaaagcta
     6181 tttatactca tctatcaact atattagttg caggatttag caccaatgta tataaaatat
     6241 actggttgcc gggtttcaaa gggccagtcc ctccaccact cttgataaga cgttatttaa
     6301 tttaattcta ttatcatttg ttgctatgtt atatatgata tattcattaa tagtatttgt
     6361 caataaattt atttaaattt tttatttttt attatcgtta attaactatt tttaattagt
     6421 tttctagctc ctaacacttt atattttaat gtctatataa ctatagaaca tcattataag
     6481 aaataaatta aataacatca atctatctaa atcatctgta aagtaaatta agaaaattaa
     6541 cattttatcc aaactctatc ctactattaa ataaaataat ttaaatacaa tgatgattat
     6601 atagctctag tactttagac tttaccaaat gtttaagttc tataggttct attattgtag
     6661 catttttccc aaaagttaaa aaatattcag ccacccagtt aagtgtagat tttggtatat
     6721 acatatctat gtatccacct ttagacaaga ttttcaaccc tcttgccaac aaaaattctg
     6781 tttcacaacg ttttactccc tcatctgtca aattaatttt aatatgataa tctgtaccta
     6841 cttccatatt cttaagataa tcatgtatag aagataaaac cttatatttc ttacttgata
     6901 agttttctat actcaaaatc ttcacaattc tgtctacacg aaattcctta atagatgctg
     6961 attttataca gtaagctgga caataccaaa gtccattcat ggcatacaaa ccaataggaa
     7021 caatggtacg tacactattt ttttgctttg atgaatattc aattttaatt gtagattcat
     7081 tcattactat attaaacaat tctttcaata atggtgttga acagtgccta tctggtgtcc
     7141 aaaaaacaaa tttacgcctt atattctcaa tattatattg aatatcattt ggtatacaat
     7201 ttaaaaattt ttttaaaaca gaaatagttt cttgctcaaa tggcaaatca ttataatatt
     7261 ctagtgactg atatgcaaag aatatagatt ttacctcact ttctgaaaat gcaatcggtg
     7321 gaagtatacg ctctttaagt acatgatatc caccagccgc tccaactttt gaatataatg
     7381 gaaatccaat actctccagc acctgtaagt ctcttaatat agtacgtttt gatacagaaa
     7441 attcttctgc taattctcct gctgtaaaac ttctttttgc atttattgtt atcatcagtt
     7501 caattaaacg ctcagctctt gacatattta tactcctaat ttaaatatag tgacaataat
     7561 tgtcactatt atatgataag atattcttac atttaaagca ataataatag taaagaaaga
     7621 agggactgat actttatgga aaataatact attaaggctg atgatattat caattattgt
     7681 ctatcaaact tagatgatgt tttattaatg aatagttggg gggaacgagc aatttactac
     7741 aatcctaatg gtattttaaa gcgaggagta tatgttctta ccattaaaga aaaagacagt
     7801 aatcatgata aaggttcgtt ggttagtcgc ccaaatgtat accgtgtgaa tataggatta
     7861 aaaaaagaaa cttttattga aatgtttgga tatattccaa aacgtccagg tgtaggtcaa
     7921 atagttgata tggattttga ttttacaaaa ttggacacaa tcatgccaca tcctatctac
     7981 tcatggatgg gatggatatg cgtcctaagc cctactgaaa agacatttga gaactttaaa
     8041 actttaatag gagaatctta caattttgca aaacaaaaat ttaaaaagag aaaaaacaag
     8101 taaaaacgta tgaaaataat ccctaaataa attataataa aaatattaag gttacaagat
     8161 aaaaattaat atcttaataa catgcaaaaa agcgaagcca aaaaatgtat tatttataag
     8221 gcttcgctat atctcaaata atttatttca aatttcaaaa ctatttattc tattatttct
     8281 tattatttcc tgttattcct aatctatcat tcatatcttt tatcattttt ctaaaaccac
     8341 tacctatatc atttccaaca tcttcagttc catgaactaa ttttataaag tcattttgac
     8401 cttttttcat atcttcatca gataccttat ctaatttttc tattctctta tctatgtctt
     8461 ttactaatct ttccccttca gcgattaggt catcattcat tttctttaac tctgtatctt
     8521 ctgttttacc atctttttta agagatgtta caaatgattg tattttatct ctactatctt
     8581 tatatgcagc ctttacatca cttagatatt tttcatttcc tggatattct ttatctttaa
     8641 actgattatt tatactttct ggagtatcat aaatagaata tctatttaga cctgctatat
     8701 aatcattata gcttgttgaa taataattca tatatccttc attattattt acagattcat
     8761 taggattttt tcctgtattt tcacttggtt tttcattatt tttagtacat gcagttatac
     8821 ctatagctaa tactagtgct aatcctacaa ataaagttct ttttaatttc at
//
LOCUS       DAMJVI010000128         8653 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.133, whole genome shotgun sequence.
ACCESSION   DAMJVI010000128 DAMJVI010000000
VERSION     DAMJVI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.133"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            32..2209
                     /locus_tag="PC545_003228"
     CDS             32..2209
                     /locus_tag="PC545_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2457967.1"
                     /translation="MYNKYNNLYLLLGKENVNMDSYRQLKAKIPCKETGIEIKRTLCD
                     ICSPGVHCGIDAYVKDGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKPISWDEAYREIAEKLNRVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLI
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQKDLIDREYIE
                     KHVHGFNEYKEYVKLFDLDTVSRITGVSKDNIVKAVEMLSQGPFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFTNSGRLFKALNSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANAMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTHLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKMNR"
     gene            2223..2645
                     /locus_tag="PC545_003229"
     CDS             2223..2645
                     /locus_tag="PC545_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2457968.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GENIEYFSLACIHCNEPKCLGVCPKNCFKKEEGFIVLDNQNCIGCKLCEKACEYGALI
                     YGNDGKANKCNGCIERYKCGMDSPCVMVCPTGALKFNK"
     gene            3053..3856
                     /locus_tag="PC545_003230"
     CDS             3053..3856
                     /locus_tag="PC545_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2457969.1"
                     /translation="MKKLKLIIMFALVSALLVTGCSSGNNKNKEEQKVIRVATSGTYY
                     PFAFKDKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYAFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKMIEPPIQFGAC
                     SFALQKNEKGEKWTKEINEVIEEMYKDGTLTKISENWLGKDITKEPINE"
     gene            3849..4517
                     /locus_tag="PC545_003231"
     CDS             3849..4517
                     /locus_tag="PC545_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2457970.1"
                     /translation="MSNFNFEFCLSIFPFLIKALGITILISIVAFFLSILLGVILAFF
                     TQTKSKVLQYITRVYVSFFRGTPLLIQIFFFYFGFPMIFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMITILIYWAVSIILSNIQSKVEKRI
                     RRAY"
     gene            4753..5415
                     /locus_tag="PC545_003232"
     CDS             4753..5415
                     /locus_tag="PC545_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2457971.1"
                     /translation="MAFNMKYFLELFPTIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDTGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            5475..6272
                     /locus_tag="PC545_003233"
     CDS             5475..6272
                     /locus_tag="PC545_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2457972.1"
                     /translation="MIKRNIIKCLVAVLSLTFVITGCSKKEEVKNEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVATSVNKD
                     NLFQEKVYGDGTSGLQDLDLGRVSAWIDAELTIVGDAKKANMKVRVLDKKLSATNIAY
                     FFKKNDDKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            6390..7580
                     /locus_tag="PC545_003234"
     CDS             6390..7580
                     /locus_tag="PC545_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDF2457973.1"
                     /translation="MEVLQMIETKIKSIALKNREKIIKIRRQIHSNPELAFKEYKTSK
                     LIKEELNKLNIEYINIAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSIN
                     DNMHACGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGV
                     DALATGHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAV
                     YSSIQQIVSRKIDPVVPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISKEQVIEVSKI
                     LEYIVKNVCKAHGADCKFEKSTGGDAVINDKNMIEIGRESACKILGAENVEIIDFPAM
                     TGEDFAIYMKEKPGLFMYIGVGDKEKNINYRLHSNKFNIDEKCLSIAPSLFSQLTVDY
                     LCQL"
     gene            7689..8438
                     /locus_tag="PC545_003235"
     CDS             7689..8438
                     /locus_tag="PC545_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2457974.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEISQSEVVAILGSSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVEKATKKDITNLRKHTSMVFQNYNLFKNKTALENVM
                     EALIVVHKKSKKEAREISLKLIDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDNGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIRKEEVFS"
BASE COUNT         3440 a          864 c         1593 g         2756 t
ORIGIN      
        1 aaatataaag tatgatattt gaataaaaag aatgtataat aagtataata atttgtattt
       61 attattggga aaggaaaatg ttaatatgga tagttataga caacttaaag ctaaaatacc
      121 ctgtaaggaa acaggtatag aaataaagag gactttatgt gatatttgtt ctccaggagt
      181 acattgtgga attgatgcat atgttaaaga tggtaaagta ataaaaatag aaggtacaaa
      241 agaacatcca atgaataaag ggttgttatg taccaaaggt ctttgcaata gagaatatat
      301 ctatagagaa gatagaataa aaacacctct aaaaagaatt ggaaaaagag gtgaaggaaa
      361 atttaaacca atatcatggg atgaagctta tagagaaata gcagaaaaat taaatagagt
      421 aaaatctgag tatggagcaa agcatgtagc tttttttacg ggttatggaa agtggtatag
      481 acctttttta caaaggtttg catcttcatt tggttcacct aattatggta cagaatctag
      541 ttcatgttca acatcagcac agatggctgg aaatgttgct actggaagaa actttacaat
      601 gcaagatatg gagaatgcaa aaacttattt agggtgggga tttaatccat actactctaa
      661 atttttaaat agtgtgaatc taataaaatt aaaagaaaaa ggtctaaaat ttataattgt
      721 agaccctaga ataacaccga caactcaaaa actagcagat ttacatttaa gaattaaacc
      781 tggaactgat ggagctttag ctcttggaat ggcgaatcta tttatacaaa aggatttaat
      841 agatagagaa tacatagaaa agcatgtaca tggatttaat gaatataaag aatatgtaaa
      901 attatttgat ttagatactg taagtagaat tacaggagtt tctaaagata atatagtaaa
      961 agctgtcgag atgttgtcac aaggtccatt ttgtataaat gaaagtgcag ctccaatagt
     1021 tcaccatcaa aatggatttc aaaattatag agcaatgatg tctttatccg ctataacagg
     1081 taattatgat agaataggtg gaaacatacc aaaacaagat tactttgctg gtaagatagg
     1141 tggattccaa acactagaaa aagagtttgt aaaagaagtt ctacctaaaa atataaatga
     1201 aagaattggt ggagaaagat ttcctctatg gaatgaaatg gtgactcaat ttcaagctat
     1261 ggatttacct aggcaaatag aagaaaagac accatatgaa ataagagctt tgttttgtca
     1321 tgggataaat gctagaatgt ttactaattc agggagactt tttaaagcgt tgaatagtgt
     1381 agattttttt gtgaatatag atttatttat gacagataca tcaaaatatg cagatatact
     1441 tttacctgca tgtacatcat ttgagagaga agaatgtaag agttatccag gagggtatat
     1501 tacatatacc aaaaaagtta ttgataaatt atacgattca aaatcagatg tagaaatact
     1561 ttcagattta gcaaatgcta tgaatataga tgatgattta ttaaaagcag gttatgaagc
     1621 aagtataaga catatgttta aaaatacatg tgtagatgta gataaactta aagaaagtga
     1681 tttaccatta aaatgtaaag attttaagcc atatatagta ggttcatata caagagaagg
     1741 gtatgatact agcacttcta ggtttgaatt aaaatctaca attattgaaa aatataaaga
     1801 ttttgggctg gatgcactac caacatatag aggatttaat gataatatag atgatgaata
     1861 tactcattta ttgacatcag gagcaagaat ttcaaatgca ctacattcaa gacttcatga
     1921 catcaaactt tcaagaatat taaaaaatga agctagttgt gatatttcta gcttagatgc
     1981 aaaagaattg ggtgttatca gtggagatta tataaaaata actactaaga taggaagttt
     2041 ggatgtaaaa gtcaatgtta ctgatagagt cttatataag aatatacata tgtatcatgg
     2101 atatagagaa gctgatataa atagtattat ttgtgataat tttgaccctt atagtggatt
     2161 ccctgcgtat agaagttgta agtgtaagat tgaaaaaatg aatagataaa tggagagata
     2221 taatgaaaag tttatttaaa tttaataaag aattatgtac agcatgtgga gcatgtttgc
     2281 ttgcatgtat agaccaaaat gataataata tagacaatgg tgaacttttt agacatgtat
     2341 ttacaataga ggaaggtgaa aatatagaat atttttcatt agcatgtata cattgtaatg
     2401 agccaaaatg tttaggtgta tgtcctaaaa attgttttaa aaaagaagaa ggattcattg
     2461 tattagacaa tcaaaactgt atagggtgta aactttgtga aaaagcttgt gaatatggag
     2521 ccttaattta tggaaatgat ggtaaggcaa ataagtgtaa tggttgtatt gagagatata
     2581 agtgtggtat ggattcacct tgtgtaatgg tatgtcctac aggagcatta aaatttaata
     2641 aataaattaa atatagagaa tgacttatac ttaggtataa gatattttta caaaaagctg
     2701 tattgagttt gttataatga aaactcaata tagctttttt atggatatag ttggatacta
     2761 taatgtgatt cttagtaata gttttttgct attggacaaa gactgtaaaa catattaaac
     2821 ttatgttgtt caaagtcgaa gtataatata ataactagaa aattggttta tagtgctata
     2881 aaagcaaatt tagtaaggta taaaatcaga ggcaatcctt tactataata tcaatatttg
     2941 ggagttctgt tattgcagag ctattaaaca attataatgg tacaagtgat tcaacctttg
     3001 aaaatgtatt atataaaaaa ttaaaataag attaaaaagg agagaataaa ttatgaaaaa
     3061 acttaaatta attattatgt ttgccttggt atcagcactg ttagttacag gatgttcatc
     3121 aggaaataat aaaaataaag aagaacaaaa agttataaga gttgcaacat caggaacata
     3181 ttaccctttt gcttttaaag ataaagataa acttaaaggg tttgaagtag atttttggga
     3241 tgaatttgct aaacgaacta attgtaaagt tgaatgggta ttaactgatt ttagtggatt
     3301 atttggatta ttagaagctg ataaagcaga tgttgtatct gctcaactta ctgcaacacc
     3361 tgaaagagag aaaaaatatg ctttttcaga tgcttataat tatagtggaa caaacattat
     3421 tgttagagaa gatgatacta gtataaaatc aatagatgat ttagttggca agaaagtagg
     3481 tgtgggtact ggagcagtag caaatgaaat attaaaagaa aaatacccaa atgatgaaat
     3541 aaaaatagta aattattcaa gtgcgactct taaagggaat tatcaagacc tagaaatggg
     3601 aagattagat gcagtagttg cgcaggatgt tgaggcttta atagctataa aagaacaaaa
     3661 attaaatctt aaaatgatag aacctccaat tcaatttggt gcctgttctt ttgcgcttca
     3721 aaaaaatgaa aaaggtgaaa aatggacaaa agaaattaac gaagtaattg aagagatgta
     3781 taaagatggg actttgacta aaatatctga aaactggcta ggaaaagaca ttactaagga
     3841 gcctattaat gagtaatttt aattttgaat tttgtctaag tatatttcca tttttgatta
     3901 aagcattggg aataacaata ctaatatcta ttgttgcatt ttttttatca atattattag
     3961 gtgtaatact tgcctttttc acacagacaa aatcaaaagt tttacaatac ataacaagag
     4021 tgtatgtttc attctttaga ggaacaccac ttttaattca aatatttttc ttttattttg
     4081 gatttccaat gatattttct tttatgaaag attgttcacc ttatttagca atagtaattt
     4141 gtttaggtgt aaattcatct gcatatatgg ctgaaattat acgagcatct attgagagtg
     4201 ttgataaagg acaacaggaa gcatgttatg cttttgggtt aagtaaatat cagtcaatgg
     4261 ttaaaataat tctacctcaa gcagctacat ctgcagtacc tcctattgta agctgtacac
     4321 tggacataat taaaggttct tctttaggtt taactatagg aatacaagat ttaatgggag
     4381 tagctcaagt agaggctgct tcaagtttta aatattttga gatatatatg ataacgatat
     4441 taatttactg ggcagtatct ataatattga gtaatataca aagcaaagtt gaaaaaagaa
     4501 taagaagagc atattagatt tcaatattta aaaatgggat gatatagata tattattact
     4561 tgtaaataaa tttttaattt tagaaaaata aacttagtaa tctaaattat tgagaaaata
     4621 tagtatcaaa atggactgta taatcaagtt aaaaaatagt ttttctattg aaaaaataaa
     4681 taaaattcta ttgtaattta atatgcgata aagttttata aaaaacaaca ttaagctagg
     4741 gggacttatt ttatggcttt taatatgaaa tattttttag aattatttcc aacaataatg
     4801 aaatcgttca atgtaactgt gattttggca ataagttcat tagtattttc attaattata
     4861 ggtacaatag tagcgttatc atcttattac aaagtgaaag tattgaatca aatatgtaaa
     4921 gtatatgtat ctatttttag aggtacacct cttatgccac agttattctt tttatatttt
     4981 ggactagcat atatgagtga ttttgttaaa aatatggacc cagtatttgc aacatctata
     5041 gtacttagtt taaatatggg agcttatatg tctgaaacga taagggcatc tatactttca
     5101 attgatacag gtcagataga agcagcatat gcaatgggat taacaaattt acaaacaatg
     5161 aaaaggatag tgataccaca ggccgtaaga gttgcactgc catcattatt caataatttt
     5221 atagatttaa ttaaagggtc ttctgtagct tttgttgttg gagtaaatga tataatgggt
     5281 gctgccaaat cacaaggagc gttgagtttt aagttttttg aagtgtatgg agcagttatg
     5341 gtaatttact ggtgtataat aactccttta ggttgggggc aaaaaaaatt agaaaagaaa
     5401 ataagcaatt attaagttat taagaaaata cataaaagta tttttatata aaaaatggtg
     5461 tgaggagaag agatatgata aaaagaaata ttataaaatg tttagtggca gttttatcgt
     5521 taacgtttgt aattactggt tgttcaaaaa aagaggaagt aaagaatgaa aaagttataa
     5581 ctgtagcttc atctaattca gctgacccat attcttatgt tgataatggt gagcataagg
     5641 gttttgaagt tgacatgtgg aaagagattg gtaaaagaag tggatataag ataaaaatgg
     5701 aaccaacagg ttttagttct atatttggta tgttagattc tggtaaagtt gatgtggcag
     5761 ccaacttctt tggaatgtct aaagaaagat tagaaaaata cgatgcttct ataccttatg
     5821 gttctgattc tgtgtgtata gctgtaaaag atggaaataa cgaaattaat aagcttgaag
     5881 atttaaaggg aaaagttgta gcagtttcag aaggttctca aggtcaacag gttgcaacta
     5941 gtgttaataa agataatttg tttcaagaaa aggtttacgg tgatggaact agtggtcttc
     6001 aagatttaga tttgggaaga gttagtgcat ggattgatgc agagcttact attgtgggag
     6061 acgctaaaaa agctaatatg aaagtaagag tattagataa aaaattatca gctactaata
     6121 tagcatactt ctttaagaaa aacgatgata aatctaaaac gataaaagaa gatgtaaaca
     6181 aagcaataga agaaatgctt gcagatggaa cagtgaaaaa aatatctgaa aaatggtttg
     6241 gaatggatgt aagtgctgat atacaaaaat aaatacttta aaattaaaat gtaaaactgt
     6301 gataactata gataagtata ctaaatttat ctatagttat ctgtgtatta caatgtattt
     6361 agattagtct ttataaatca aaatgagtta tggaggtatt gcaaatgatt gaaactaaaa
     6421 taaaatccat tgcactaaaa aatagagaaa aaattataaa aataagacga cagatacaca
     6481 gtaatccaga gttagcattt aaagaatata aaacatctaa acttataaaa gaagagctaa
     6541 ataaacttaa tatagaatac ataaatattg ctggtacagg agtattagct actataaaag
     6601 gaaaaaataa tggtggaaaa actatattac tcagagcgga tatggatgct cttccaatca
     6661 aagaggaaaa tgatttagaa tttaaatcaa taaatgataa tatgcatgct tgtgggcatg
     6721 atgcacatgt ttcatggttg ttaggaacag caatgatttt aaatgacata aaagaagaat
     6781 tgaatggaaa tgtaaaatta ctatttcaac ctggcgaaga aaaaggtggt tctgatattg
     6841 taattaaaga aaatgtactt gaaggtgtgg atgcattagc gactggtcat tgttggccaa
     6901 ctattgagtc aggaaaaatt ggaatcgcta gaaattgtgc tatggcagcg actaatactt
     6961 ttgaaataac aataattggc aagggtgggc atggagcaga gcctcataat tgtatagacc
     7021 caattgcagt tggaaatgca gtatattcat ccattcaaca gatagtttca agaaaaattg
     7081 acccagttgt acctgtagta gtgtctgttt gttcttttaa ttctggtgtt tctaaaaata
     7141 taataccaga tgtatgtaca cttcaaggga ctattagagc gataagcaag gaacaagtaa
     7201 ttgaagtaag taaaattcta gaatatatag taaaaaatgt atgtaaagca catggagcag
     7261 attgcaaatt tgaaaagagt acaggaggag atgctgttat aaatgacaaa aacatgatag
     7321 agataggaag agaaagtgct tgcaaaatac ttggagctga aaatgttgaa ataattgatt
     7381 ttcctgcaat gacaggtgag gattttgcta tttatatgaa agaaaaacct ggtttattta
     7441 tgtatatagg tgtaggagat aaagaaaaaa atataaatta cagattgcat agtaacaagt
     7501 ttaatataga tgaaaaatgt cttagtatag caccttcatt attttcacaa cttacagtag
     7561 attatttatg tcaactatag agtacattca taaaaatata tttagcctat ttttaaattt
     7621 aatgctagta tattaatttt gatgtagaac atattattta ttatatttat ttaaaaaggt
     7681 ggataacaat gataaaagta gaaaatttac ataaaagttt taaagggaca gaagttctta
     7741 aaggtataaa tttagagata agtcaaagtg aagtagtagc tatacttgga tcatctggaa
     7801 caggtaaatc tacgttactt agatgtttaa attatttagt tgagcctaca aaaggaaaga
     7861 taatgattgg tgatgtaaaa gtagatgtag aaaaagcaac aaaaaaagat ataaccaatt
     7921 taagaaaaca tacatctatg gtgtttcaaa attataactt atttaaaaat aaaacagcat
     7981 tagaaaatgt aatggaggca ttgatagtag ttcataaaaa gagtaagaaa gaagctagag
     8041 aaatatcttt aaaattaata gataaagtag gtatgttgga taggaaagat ttttatccat
     8101 caaagttgtc tgggggacag caacaaagga taggtatagc gagagcgatg gcagtaaatc
     8161 caaatgtaat attatttgat gagcctacat ctgcacttga cccagaactt gttggagaag
     8221 ttttaagcgt tataaaatct cttgcaaaag aaggtataac gatgcttatt gtaactcacg
     8281 aaataaattt tgcaaaagaa gttgctgata aaattatatt tatggataat ggtgtaatag
     8341 ctgaagaagg aactcctgat gaaatattta ataatcctaa aaatgaaaga acagcaaaat
     8401 ttttaaatat cattaggaaa gaagaagtat ttagttaaaa gtgtataaat taagcattag
     8461 aagatatgta agataccccc attaaattta taagttagat aataaattta atgggagtat
     8521 atttttatta ttagatttat tatttaattt aaaattagaa atgtaactat ttgaaaattc
     8581 tttttaaaag tatatcaaaa tatcaaatac ataaaaaaat aatatttgac aaagtatcat
     8641 atgtgtttac aaa
//
LOCUS       DAMJVI010000129         8548 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.134, whole genome shotgun sequence.
ACCESSION   DAMJVI010000129 DAMJVI010000000
VERSION     DAMJVI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.134"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..889)
                     /locus_tag="PC545_003236"
     CDS             complement(<1..889)
                     /locus_tag="PC545_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2457975.1"
                     /translation="MKDKNTYFNRKGQENFIDIFLKDDIKKGANFKRHWHEHLQIYYF
                     IDGIAYLECGLDNFKVVPGNIVIANSNELHYLESLSDNLKFYSIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSLILKCITTILDEYHNKEVGYELSIKANIYQLIVL
                     LLRSHIDKIYSEDEFIKRKYSLEKFECIFDLIEEKYMDKITLKELASRVNLSAHHFCR
                     TFKQITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKKNYNI
                     SPTKYRNEHREVSNVILKED"
     gene            1030..2457
                     /locus_tag="PC545_003237"
     CDS             1030..2457
                     /locus_tag="PC545_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457976.1"
                     /translation="MTNSFKKDDNRKKITVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLASIEWVIASYSIIICSTLLFFGRLGDIIAKSRVFQVGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKESRGKALGILVTAVAIGNMVGPSVGG
                     FILSIFNWNVIFFINIPIGLIVIFLNTKFLPNSKKSSENMDKTGAILQFLGTTLFFSA
                     LISAQQTGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQSTMKLPPIQAGLFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLVMSFGFFLMSRLKESSALILFVIYISIISIGQAIFQPANNALIMSSCSRSKL
                     GVVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYVINNDKIFVFGMRNVYI
                     IVTLVCLIGAILIGFYLFKYNKNEQ"
     gene            complement(2653..5247)
                     /gene="clpB"
                     /locus_tag="PC545_003238"
     CDS             complement(2653..5247)
                     /gene="clpB"
                     /locus_tag="PC545_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDF2457977.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSHRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETIIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5284..5880)
                     /locus_tag="PC545_003239"
     CDS             complement(5284..5880)
                     /locus_tag="PC545_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457978.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIAKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5897..6301)
                     /locus_tag="PC545_003240"
     CDS             complement(5897..6301)
                     /locus_tag="PC545_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HDF2457979.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6668..7042
                     /locus_tag="PC545_003241"
     CDS             6668..7042
                     /locus_tag="PC545_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2457980.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7039..7902
                     /locus_tag="PC545_003242"
     CDS             7039..7902
                     /locus_tag="PC545_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2457981.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWNDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHKPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            7902..8540
                     /locus_tag="PC545_003243"
     CDS             7902..8540
                     /locus_tag="PC545_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HDF2457982.1"
                     /translation="MKGLILKDLLNLKGNIKFILLFIIMFGFMSSLGDGNVNNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYSKKEV"
BASE COUNT         2862 a         1268 c          999 g         3419 t
ORIGIN      
        1 tatcttcttt taaaataaca ttactaactt ctctatgttc atttctatac ttagttggag
       61 atatattata attcttttta aataagcgac taaaataatt tatgctatca aagccacatc
      121 tcatagcaat ttctgtcata tttaaatctg tttcctttaa ataatatata gctttatcaa
      181 gcctgattcc atttataaaa tcgattgtag tcttacctgt tatttgctta aatgttctac
      241 aaaagtgatg tgcactcaag ttcacacgac tagcaagttc ttttaatgta attttatcca
      301 tatatttttc ttctatcaaa tcaaagatac attcaaattt ttctaaagaa tactttcttt
      361 ttataaattc atcttcagaa tatattttat caatatgact tcttaataag agtactatta
      421 attgatagat attagctttt atagataact catatccaac ttctttattg tgatattcat
      481 caagtatagt tgtaatacat tttaaaatca gagaatcatt ttgaattaaa ttattaaata
      541 caatatgatt taaagccaat ggtgctaagt acttggtttg taacaaatca acttgattac
      601 taaaaaggaa tgtgggttca atacgaatac tatagaattt taaattatct gataagcttt
      661 ctaaataatg taattcatta ctgtttgcta ttacaatatt tccaggcact accttaaaat
      721 tatccaatcc acattcaaga tatgctatac catcaataaa ataatatatt tgtagatgct
      781 catgccaatg tcttttaaag tttgctcctt ttttaatgtc atcctttaaa aaaatatcta
      841 taaagttttc ttgacctttc ctgttaaaat atgtattttt atccttcatt attcccccct
      901 catcaaaata gtgctaatta taagcaatat agttattgta ttcattattt catatcgcta
      961 ttatataaaa tgtaactatc attgtaaaca ttaacataat gcagtcatta taataaggag
     1021 gtaataaaaa tgacaaactc ttttaaaaaa gatgataata gaaaaaaaat cacagtttta
     1081 tttgtagtca ttgccatgac ttttatggca acattagatt ctagtattat aaatgttgct
     1141 ctcccagttc ttgcaagcaa actaaatgta tctctagcat ctattgaatg ggtaatagca
     1201 agttactcaa ttattatttg ttcaacacta ttattttttg gaagacttgg agatattatt
     1261 gctaaatcaa gagtatttca agtaggtact attctcttta catcagcttc attactatgt
     1321 ggattaagta attctttaac tttacttata gtttgccgct ttattcaagg aataggcgct
     1381 tctgcctaca tggcaaacaa tcatggaatt atcacagaac tatttccaaa agaaagcaga
     1441 ggaaaagccc tagggatttt agttactgca gttgctattg gaaatatggt tggaccatct
     1501 gtgggaggat ttattttgtc aatatttaat tggaatgtta tattttttat aaatattcca
     1561 atcggtctta ttgtaatctt tttaaataca aaatttcttc ctaatagtaa aaaatcttca
     1621 gaaaatatgg ataaaactgg tgctatactt caatttttag gaacaacatt attttttagt
     1681 gctctaatta gtgctcaaca aacaggatta ctaaatccat atatattaat tgcactatta
     1741 ttatcaatta tttttattat attatttttg atattagaaa aaaaacatcc tcaaccgctt
     1801 cttgaccttg aaatttttag aaattttaaa ttttcattga atttaatttg tgctcttaca
     1861 tcattcattt gtattgcatc atcaagtatt ttaattccat tttatttaca aagtactatg
     1921 aaacttcccc ctattcaagc aggtttattt atgatactat cacctttgat tttagccatt
     1981 ttttcaccta tatttgggaa tatatcagat aaaattaagt cagaaaaaat tattttaatt
     2041 ggtcttctcg ttatgagttt tggattcttt ctaatgtcaa gattgaaaga aagttctgcc
     2101 cttattttat ttgtaattta tatatcgata atatcaatag gacaagctat atttcaacct
     2161 gctaacaatg ctttgataat gtcaagctgt tctagaagta agctaggagt tgtaggaagc
     2221 ataaattccc ttgtaagaaa tttaggacaa gttataggta ttactatttc tacaacactt
     2281 ttatataatt ttatgagtat taaagctgga tatagggtca atgattatgt gattaataat
     2341 gataaaatat ttgtatttgg aatgagaaat gtttatatta ttgttacact tgtttgctta
     2401 ataggtgcaa tattaattgg cttttattta tttaaatata ataaaaatga acaatagttt
     2461 ttataattat attcaaatta tgattatatt ataagaaatt tactaaaata cttcctttga
     2521 gttaaattta atttggacat attgacttaa aaatgtttta ttaatttgat aaaatatttc
     2581 ttttcctata gtttataaat aaaaatcagc tgagcaaaat atttagtata ttatgctcaa
     2641 ctgattcttt tattatttaa ctaaaacttc aatattttca tcttttccat ctataactat
     2701 agtacatcca ttatacacat ctccagcaat aagtttcttt gcaattatag tttctaatgt
     2761 atttcctata tatctcttaa gaggtcttgc tccatataca gggtcatatc cttctctcac
     2821 cataacatct tttgctgaat ttgtaacttc aatagtaata tctttttctt ttaatctatt
     2881 ttttaaatct ttcataaata tatctataat tttcttgata ttttcttggt ctaaaggctt
     2941 aaacattata atatcatcta ctctgtttaa aaactcaggt ttaaatctat gcttcatttc
     3001 attcattact aaattgttag tagtctctgt tatatttcct cctgcttcta atagataatt
     3061 acttcctata ttagaagtca ttattataag agtattttta aagtctactg tcttaccttt
     3121 attatcagta agtctaccat catctaatat ctgtaagaac atgttaaata catcctcatg
     3181 tgctttttct atctcatcaa atagtattac agaataagga gcacgtctaa ctgcttctgt
     3241 aagttgacca ccctcttcat atcccacgta ccctggagga ggtccaacta gtcttgatac
     3301 agcatgtttt tccatgtatt cagacatatc tattcttatt atattatctt cactatcgaa
     3361 taaatttcta gcaagagttt ttgcaagttc agttttacca acacctgtag ggcctaagaa
     3421 aataaatgaa ccaattggtt ttctttcatc ttttaatcct gctcttgcac gtattactgc
     3481 atttgaaact gctgtaactg cttcatcttg tccaataact cttttatgaa gttcatcttc
     3541 tagtttaagt aatttttctc tttctccttc aactagttta gttactggta tacctgtcca
     3601 ctttgataca atgctagata tttcttcttc agtaacttct tcttttaaaa gagaattttc
     3661 agaaccatca ttcatatttt cttcgtactg ctttatttgt tcttctaact taggtatttc
     3721 accatattta acctctgctg ctttgttgaa atcatattct ctttcatact tttcagcttt
     3781 acctttagct tcgtctaatt gagcctttaa gtttttaata tctaatattt gactcttttc
     3841 tttttcatat tttgctgtca tttcatcatt tttagatttt aactctgcta tttctttttg
     3901 aatatcatcc aatcttgatt tacttttatc atcattttct ttaagtaatg cttctctttc
     3961 agtttctaat gtaaataact ttcttctaac aacatctaat tctgttggca atgaatctat
     4021 ttcactacga atcatagctc cagcttcatc aattaaatca attgctttat ctggtaaaaa
     4081 tctatcttgt atatatctat gagaaagttt tgctgctgct acaatagcat tatcatgtat
     4141 tcttactcca tgatgtattt caaatctttc ttttaaacct ctaagtatag atattgtatc
     4201 ttcaacagta ggttcttctg caattacagg ctgaaaacgt ctttcaagag ccttatcttt
     4261 ttctatatac tttctatatt catcaaaagt agttgcacca atacagttca attcacctct
     4321 tgcaagcata ggtttaataa gattacctgc atccattgaa ccttctgttt taccagctcc
     4381 aacaatagta tgaatctcat ctataaacag tataattttt ccatcagaac tttgaacttc
     4441 ttttaaaact gcctttaatc tttcttcaaa ttcacctcta tattttgcac cagcaattaa
     4501 agctcccata tcaagtgaga atacaatctt atcttttaaa ccttctggga catctcctct
     4561 tactattctc tctgctaacc cttctacaat tgcagtttta cctacacctg gctcaccaat
     4621 caatacagga ttattttttg ttcttcttga taaaattctt ataatccttc ttatttcttc
     4681 atctctccca attacagggt ctaatttatt tttcttggct aaatctacta agtttgttcc
     4741 atatctagca agtgcatcat atgtgccttc tgggtcttgt gtttctactc tttgactacc
     4801 tctcacttgt gataaaatat ttaaaaagtc attcttattt atattatatt gttttaatat
     4861 tttaccaaca gctgattttg attctgtttc catcattgca agcataacat gttctacact
     4921 tatatatgaa tctttaaatt ctttagaaat actctctgcc tttataagca cctcatttat
     4981 ttttctagta gctgatactc cttgtgagtc agcaccttct ccatatactt ttggaagctt
     5041 atcaatttca aagtttacag tatttctcaa agaatcaatt gatatattca ttttttcgat
     5101 tatatttggt atcaatccat cctcttgatt aataagtgct gagaaaagat gaattacatc
     5161 tacttgttgg ttatgatttt ttactgcttc gttataagcc tcatttaaac ttttttgaac
     5221 tcttaaagtc attttttcta cgtccatata aatccctcca ttttaatatt atatccaaat
     5281 aaattaaatt ctctcattta taattattga ttttctaagc tctaaaagac tatcaagtaa
     5341 attctcgttt ttttgtatat cgaaactcaa tgttaaatta aatgttccat ctgaattaaa
     5401 aaaatcgaat acttcttttg attttgctat taaatacaag tcttcatcta taagtctttt
     5461 ttcacctatt aataaatact gacatttatc tataaaactc tttttaaaat caatagtgta
     5521 tagattttct aaaatattat attgataaat agcaatatta ttaaacactt ttaatatgtt
     5581 tgaatttaca aaattgtaaa tttgattatt gttatacagt gatatttgtt cgtatatata
     5641 ttccctaaat aaaggatgaa tgttttgctt aagttttaaa gtaacttcag ctggtatttc
     5701 aaaataaagc ttccagtact tagagttatt tttttctaaa ataaaagcta ctttgttcaa
     5761 actggtagaa ctaatctcct ttagtgtaca attaaaatta tagtgtctca aactagtttt
     5821 ttcattctca aatacatagt caaaccactg tagaacattt ctatcaagtt ttaaactcat
     5881 ataaaaacct tcttccttat ttactaacct tattaggtct ttttatctga ttatattctg
     5941 ctaataaact ttctttatca gctctactac ctttatatgc tatatgtgga tttatcatat
     6001 actcaccatt tttcactctt tgtaaaaaat ccattttttt cagctccgta aacaatgaag
     6061 ataaagaagt ttttggatat tcaaattcac aagataattc atataatgtt tttctaatct
     6121 tattattttt atccatagag tttattaatt ttaatggaat taaatccctc ttagtataca
     6181 ttccttctac tactttaata taattcttaa aacaaacatt catatactta cttttattca
     6241 taactaaact tttgctctct gtatattcct gatttaaatc cttttttaat gtcttatcca
     6301 aattaaatat ctcttctcac tctccttatc tctaattgat aatataaata tattacaaaa
     6361 aaatatgtat gtcaaaactg aaaatgcaaa aagttcacct tataagtaaa cttttattta
     6421 gctataagct aaactttatt atttttttaa tttagtattt ccaatttatt ggcaggattt
     6481 ttgaacaata tttataaatt ttttaaaaaa atttaaatct atttaatatg gcagactttc
     6541 ataaaataca ttacatttat taaatgtata atattggcaa ttagtttttt ctaaaatcag
     6601 ttgacatatt atatatatat ttatataatg tgtatatact atatatacaa tttaaggagg
     6661 tgtcttattg aatatagtta ttagtaattc gagtgggaag ccaatatatg aacagattac
     6721 tacacaaatt aaaagtatga ttataagtgg agagttacca gaaggctcag ctcttccaag
     6781 tatgagacta ttggcaaaag aacttagaat aagtgtaatt actacaaaac gagcatatag
     6841 cgatttagaa cgagacggat ttattgagac agttactggt aaaggcagtt ttgtatctag
     6901 taaaaacatg gattttgtca aagaagaaca atttcgttta attgaagaat acctacaaaa
     6961 agcagttgat acagcaaaaa gtagtgatat tacatatgac gaattaaaag atattttatt
     7021 tttattatat aaaggagaat gataagtatg gatgcaataa ctataaaaaa tttaaataaa
     7081 acttataaag attttagcct acaagatata tctttttcag taccaaaagg gagtgtaatg
     7141 ggatttgtag gtgaaaatgg tgcaggaaaa acaactacat taaaagcaat tttaaattta
     7201 atatcttatg atagtggaaa tatagaaatc tttggtcttg atagtaaaaa aaatgaaaaa
     7261 gaaattaaag agcaaattgg ggttgtattt gagggaagca actttcatga aaatttaaac
     7321 actgaccatg tttcaaaaat catgagtaaa atctataaaa attggaatga cacattattt
     7381 aaagactact taaaaaaatt gagagtacca gacaataaat taattaagga gttttcaaag
     7441 gggaataaga tgaaacttag tattgctgta gccttatctc ataaaccaaa attattaata
     7501 ctagatgagg caactagttc acttgaccct atcgttcgtg aagaaatact agatatattt
     7561 ttagatttta tacaagatga agaacactct attatcttat cttcacatat tacaagtgat
     7621 ttagataaga tagcagacta tattacattt atacataaag gaaaaattgt atttagtgaa
     7681 aataaggatg aattaattga tactatggga gttttaaaat gtaaacctag tgattttgat
     7741 aacctttcaa gagaggatta ttcatattat cgtaaaagcc aatttggata tgaagtactt
     7801 cttaaagata agcatagatt tatctcaaga catccaaact gtattgttga taatacgtct
     7861 atagaagaaa ttatgttatt ttatgtgaga ggtgataaat aatgaaaggt ctaattttaa
     7921 aagatttact taatttgaaa ggaaatataa agtttatact tttattcatc attatgtttg
     7981 gctttatgtc ttctttagga gatggaaatg ttaataactt tataggtgtt attatagttt
     8041 tatgtactac aatgattgtt tcaacatttt catatgatga tttgaataaa tgggatagtt
     8101 atgtacttac aatgcctatt aatcgcaatg atattgtatt aagtaaatat ttaactatgt
     8161 taatatttag ctttataggt gttcttgttt cacttattgt tagtgtaact attggttatt
     8221 ttaaaaatac acttatatta aatgaaacac tacttattaa tgcactaatc ttatcaattt
     8281 cagtatgttt tggtagcctt attctaccac ttatttataa atttggtaca gaaagagctc
     8341 gtttattaat gattttatgc tttttagttc caacactagc tttattagtt tttaaaagta
     8401 ttttagaaaa tattagttca cctatttcta tagaaattat acttaataca ctagtatatt
     8461 ctcttccatt tgttgctatt ttactatttg tgatttcata ttttatatca tcaaaaatct
     8521 atagtaaaaa agaagtataa aatatact
//
LOCUS       DAMJVI010000130         8509 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.135, whole genome shotgun sequence.
ACCESSION   DAMJVI010000130 DAMJVI010000000
VERSION     DAMJVI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.135"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            244..1230
                     /locus_tag="PC545_003244"
     CDS             244..1230
                     /locus_tag="PC545_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDF2457983.1"
                     /translation="MKKITINDIANLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIITPCLDSFVKSKIMMAIDEELKKLKYTSLIINTNRKI
                     RSEIDSISKLASLKVDGIILIGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIANKGYKNIVYLGVDESDISVGLNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     EMSIQRSGEMLESENPDIIICATDNIAIATMKEINKRGKSIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENKNAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            1399..1547
                     /locus_tag="PC545_003245"
                     /pseudo
     CDS             1399..1547
                     /locus_tag="PC545_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
     gene            1566..3047
                     /locus_tag="PC545_003246"
     CDS             1566..3047
                     /locus_tag="PC545_003246"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDF2457984.1"
                     /translation="MYKEPKYRTILESTKEELNKLRNISLNDKYKPLFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDATHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNAIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMNKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSDKKGVIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDEKDILIFSPQGVEPKGFDYQNIYNVVYAIGNMDLDNLTFEIDTMK
                     ELEKGFDFYAPQTFIKDSQIILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELIKLRYDETSGQNTIKNNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKLGNIVTLDRSNFKKVFGVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NEGEVVFTSRIFAKENSNQIRVYSDKIVEYEYTKFKLKQGIEL"
     gene            3073..4044
                     /locus_tag="PC545_003247"
     CDS             3073..4044
                     /locus_tag="PC545_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDF2457985.1"
                     /translation="MKKVISIGEALIDFIPNQNGGKLKNVSEFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHILHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSEQSCKKAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGVKVNAIDTTGAGDSYIGSFLYTLLYKQITLN
                     DIKEMKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEITEFIKNL"
     gene            complement(4300..4902)
                     /locus_tag="PC545_003248"
     CDS             complement(4300..4902)
                     /locus_tag="PC545_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HDF2457986.1"
                     /translation="MEVCILMGSPKKKGNTAAILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKILMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWAGKSVALITTCGYRPEKGADIWENGMKRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYYILSNSIELDNC"
     gene            5183..6394
                     /locus_tag="PC545_003249"
     CDS             5183..6394
                     /locus_tag="PC545_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HDF2457987.1"
                     /translation="MYSNEDYKFIEENAPCYIYDETNIIEKCKELKNNILGFDFLYSI
                     KTNPYNKIVKSIAEQGFGADASSTNEVMISSKCGVSKDDIYYSSPGKTEIDIETVLDK
                     CIIIADSITEIELINKISKKHNQKVKIGIRINPDFTMDDDSAKSSKFGIDIEDMSSVF
                     KMLSMCDYVSVVGIHIHIKSQVLDFEKLGHYYEKCFFIALNLNKSKYINIEFINFGSG
                     LGVAYDNLRENPLNLKELSKLTQKIAFTNSNTLKSRLLIESGRFIVCKSGNYYVKIVD
                     IKVSRGKKYLIVKNGLNGFMRPIISNLIENITCGVELEGQEPLYTSNHAFSLSVLNNL
                     KEEEYVNVVGNLCTSLDVIANNVKLKKANIGDLISISNAGSYSYSLSPLLFSSYEFPK
                     QYYKRKDGYIE"
     gene            6462..6659
                     /locus_tag="PC545_003250"
     CDS             6462..6659
                     /locus_tag="PC545_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HDF2457988.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            6693..7664
                     /locus_tag="PC545_003251"
     CDS             6693..7664
                     /locus_tag="PC545_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2457989.1"
                     /translation="MLLRASIGMGIVIFTMAFVQNVYQLLGLRILQGVFTGYATACTT
                     LIATQTDKNHSGWALGTLATASTTGSLIGPTVGGYIESILGLKSTFIITGGLLFVSFI
                     ISILFVVDNFKPKEIKESISIPKEQLNILPNKFLIASIFVTTFITQLALYSIEPIVTI
                     YISQLTNFASNVALIAGLTFSASGLANLLAAQKLGKMSDKIGPQKVLLISLLWAGIIF
                     IPQAFVKTAWQLMLLRFLLGLSVAGLNPSVNSLLKKIAPEEYVGKIFGYNASAQYIGC
                     SSGAFLGGQISAHLGIRTVFFSTSLLLFRNALWMYKFTGLFIKDKTK"
     gene            7851..8228
                     /locus_tag="PC545_003252"
     CDS             7851..8228
                     /locus_tag="PC545_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2457990.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMERKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFESHFKNLEEE"
BASE COUNT         3462 a          887 c         1359 g         2801 t
ORIGIN      
        1 cttattttta tattataaat aaattagagt atttatcaat tcatagtaga taaaattgaa
       61 attaaatata acaacaaaaa atgaaaatta caattaattt aatgttaaag actcaattat
      121 ttggatgtgg aatggatagt acaagtagta tataaaaagt attgactgga caaataacta
      181 tattttattt taaatatata atataatgta ataaaatata tcaaatgtag aaggtgataa
      241 cctatgaaaa aaataacaat aaatgatatt gctaacttgg caggagtatc taaaagtact
      301 gtatcaagat atctaaacaa taaagatatc agtgattcta ctaaagagaa aataaagaca
      361 ataatagatg aatatggata tgagccgaat gcatttgctc aaagtttgag agctaaaaaa
      421 acatatttta ttggtattat aacaccgtgt ttagattctt ttgtaaaatc taaaattatg
      481 atggcaatag atgaagaact caaaaaatta aaatatacat cactaattat taatactaat
      541 agaaaaataa ggtcagaaat agatagtata tcaaagttag ctagtctaaa agttgatgga
      601 ataatactaa ttggtaccga aataactaaa gaacataaga atgaaattga aaagttagat
      661 ataccgatag ttgtagttgg acaaaaagtt gatggtatca atagcatagt gaatgatgat
      721 tatggagcag gctataaaat gggtcaatat attgctaata aaggttacaa aaacattgta
      781 tatttaggtg ttgatgaaag tgatatatca gttggtttaa atagaaaaaa tggagtactt
      841 aatgggttaa aagataaagg gtatgatgca aaagtatttt atactgactt tgaccaagaa
      901 atgtctatac aaagaagtgg agaaatgcta gaaagtgaaa atccagatat aataatatgt
      961 gcaactgata atatagcaat agcaacaatg aaagaaatca ataagagagg taaaagtata
     1021 ccacaagata tatctgtagc tggatttggg ggatatgatg tattgtctat tataagccct
     1081 aaacttacaa caataaagtt tgaaaataaa aatgcaggta aagtagcagc aaatacgata
     1141 gtaaatttaa tacaagaaag aaaagaacca ctattaaagg agattaaatt tgaattaata
     1201 gaaggtgaaa gtacaataaa taaaaattaa gatacacatt acatatatta gtaatgtgta
     1261 tttttttact aaaaaaacta taattctaaa aattaggaaa acttttttga aagaaatatt
     1321 gcatctgaaa taaatttatg ctaatcttta attaaaccgg tttcgaaacc ggtttaatta
     1381 aaccgataga atagcaaaat gagtgcaaaa ataactgatt gtaacaaagt atcaaagtaa
     1441 ttattggagt acataggaat aaacaaaata taaaagatgt agcacattgt gtaataagac
     1501 taagaattgt tttagatagc aataataaag caaatataaa attataaaag aaaaggaata
     1561 aaaatatgta caaagagcct aaatatagaa ccatattaga atcgacaaaa gaagaattaa
     1621 ataaattaag aaatatatct ttaaatgata aatataaacc actatttcat atacatccac
     1681 aacatggact acttaatgac ccaaatggat tagcatatta taatggtaag taccatgtat
     1741 tttatcaatg gtatccatat gatgcaactc atggaatgaa gcattgggct tatgtaagtt
     1801 ctgatgattt tgtaaactgg aatagagaag atgtagcatt aataccaatc gaaagttatg
     1861 aatctcatgg agcatattca ggtaatgcga tagaggtaga tggaaaattg catatgtatt
     1921 atacagggaa tataaaatat agtgcagaag atagatatgc atatcaaaac ttagctataa
     1981 tgaacaagga tggtaaaata actaaatatg aaaataatcc aatagtaagt gaaataccaa
     2041 aaggatatac aggacatgta agagacccaa aagtatttaa aagaaaggat aaatacttta
     2101 tgttacttgg agcacaaaca agtgataaaa aaggagttat aatagtatat gaatctaaaa
     2161 actctataga ttggaatttt aaaggagaat taaatgtaaa aaacatagat gaagattttg
     2221 gttacatgtg ggaatgtcca gattatatta atatagatga aaaagatata ttaatatttt
     2281 caccacaagg agtagaacct aaaggatttg attatcaaaa tatatataat gtagtatatg
     2341 ctattggtaa tatggattta gataatttaa catttgaaat agatacaatg aaagaactgg
     2401 aaaaaggatt tgatttctat gctccacaaa catttataaa ggactcacaa ataatactat
     2461 ttgcatgggc tggaatggga gaagttttat accctacaga taaaaacaaa tgggcacatt
     2521 gtctaactgt tccaagaaaa ttaaatataa aaaataataa gctattgcaa atgccagtag
     2581 atgaattaat taaattaaga tatgatgaaa caagtgggca aaatactata aagaataata
     2641 ttaatattat agaaaatgat gaaaatgtat atgagcttaa tattaatata aaaaatattg
     2701 acagcaataa atttgggctg gaattatttt catcacaaga tgaaggtgta aaattagaat
     2761 ttaacaaatt aggaaacatt gtaactttag acagaagcaa ctttaaaaaa gttttcggtg
     2821 ttgaatatgg aacaaataga aaagaatata taaatattga tgaaaatact aatatcaaag
     2881 tactagcgga tagaagtatt ttagaaattt ttataaatga aggagaagta gtatttacta
     2941 gtagaatatt tgctaaagaa aattctaatc aaataagggt ttatagtgat aaaatagtag
     3001 agtatgagta tactaaattt aaattaaagc aaggtattga attataaaaa cacattataa
     3061 ggggtagaaa agatgaaaaa agtaattagt ataggagaag ctttaataga ctttatacct
     3121 aatcaaaatg gtggtaaatt aaaaaatgta tcagaattta gaagagtagc aggaggagct
     3181 ccagcaaatg ttagtgctgt tgtagcaaag cttggaggaa aatcgagttt tatatctaag
     3241 ttaggaaaag atgcatttgg tgattatata atagatgtat taaatgaagt aaatgtaaat
     3301 actgattatg tattgagaac aagtaaagca aatacaggac ttgcttttgt atcattaaaa
     3361 gaagatggta atcgtgattt ctcattttat agaaatccaa gtgcagatat gcttttagaa
     3421 gctgatgaag ttaaaaaaga gtggtttaat aattgtcata tcctacattt ttgttcagta
     3481 gatttaatag atagtccaat gaaattagca cataaaaaag caatagaata cgcacttgaa
     3541 agtaatagta taataagctt tgaccctaat ataagattac ctttatggga tagtgagcaa
     3601 tcttgtaaaa aggcaatatc agaattttta ccttttgctc atattgttaa gatatctgat
     3661 gaagaattag aatttataac aggagaaaat gatatagaaa aatcattaca taaactcttt
     3721 gtggggaatg ttgaactagt attatataca aaaggaaaag atggagtaga tgcatacact
     3781 aaaaaagtaa aaggtatgtg taaaggggtt aaagtaaatg caatagatac aaccggagca
     3841 ggagattctt atataggttc atttttatac accttattgt ataaacaaat aacattgaat
     3901 gatataaaag aaatgaaaca agaaacgtta aatgagtatt tagaattttc taattattat
     3961 gcagctcgta gtacaactat taaaggtgcg ataagttcat atgcaacaaa ggaagaaata
     4021 actgaattta taaaaaattt atagtaggaa tgagtaatag gttttgaagg aagtaacatg
     4081 taaagagttt ttaaggatgt ttactatgaa aataattaac atataaaaag caataaggag
     4141 gctgtctcaa aatagagata agcctcttta tcattgttga aatgataaaa aatattttaa
     4201 ttattgttac tttttagaag aatatatatc ttattttaaa taagcaaatt atgtaagatt
     4261 tatttatatt atcaatactt tacttggagt attatgtctt tagcagttat ctaattcaat
     4321 tgaattagat aaaatataat aaatttgttt tgcaaattga cgagaatgtt cttctttttc
     4381 tttattcata aaattaggtt tacctaaatc acgttcaaca agcattccta tatattttaa
     4441 ctgtgaatgc ttacagtagc gcttcatacc attttcccaa atatcagcac ccttttcagg
     4501 tctatatcca catgttgtaa taagtgctac tgattttcct gcccatagag cggggccttt
     4561 ctcattacca tagtatttat tcattccata caccaatctg tcaagtagtg ctttcattgg
     4621 agaagtgcag taccaagaat aaattggagt agcaaaaaca attaaatcac acataagaat
     4681 tttcttgaaa attttctgaa catcatcttt acaataacat ccaaaatcaa ataagttatc
     4741 ctgacacttt ttacaagcaa tacatggctc tattttacaa tcatataacc atattacttc
     4801 aatatttgaa ttataatttt ctaattcctc aataaaaggt tttaaaatag cagcagtatt
     4861 accttttttc ttaggacttc ccataagaat gcatacttcc atattgatac ctccacagta
     4921 ttttaaaata gtttttagtg cttaatatag ttttaggata ctatgagaat acgttaacaa
     4981 caatacactt tgtatatttg taataaaatt gaaacaaatt gttttatcaa taaaagttgg
     5041 aatgtatcta taattaaaaa tattttaggt atgtttttac atatatgtgt gtagttaact
     5101 atggtaattt attgtaaaat tatcttatag gattataatt gcttaattta tgattatatt
     5161 taaaatcttg gaggtaaaaa atatgtactc aaatgaagac tataaattta tagaagaaaa
     5221 tgcaccatgt tatatatatg atgagactaa tataattgaa aaatgtaagg aattgaaaaa
     5281 taatatttta ggttttgatt ttttatattc tataaaaaca aatccatata acaaaattgt
     5341 aaaaagcatt gcagagcaag gttttggagc agatgcttct tcaactaatg aagttatgat
     5401 ttcatcaaaa tgtggagttt ctaaagatga tatttattat tcatcaccag gcaaaacaga
     5461 gattgatata gaaactgttt tagataaatg cataattatt gcagatagta ttacagaaat
     5521 agaacttata aataagatat ctaaaaagca taatcagaaa gttaaaatag gaataagaat
     5581 aaatcctgat tttactatgg atgatgatag tgctaaatca agcaaatttg gtatagatat
     5641 agaagatatg agcagtgttt ttaagatgtt aagtatgtgt gattatgtat ctgtagttgg
     5701 gattcatata catattaaat ctcaggtatt ggattttgaa aagttaggac attattatga
     5761 aaaatgtttt tttatagctt taaatttaaa taaaagtaag tatattaata ttgaatttat
     5821 aaattttggt agtggacttg gagtagctta tgataatctt agagaaaatc cacttaatct
     5881 taaagagctt tcaaaattaa ctcaaaagat tgcttttact aattcaaaca cactaaaatc
     5941 aagacttctt attgaatcag gacgtttcat agtttgtaaa tcaggtaatt attatgttaa
     6001 aattgtagat attaaagtat caagagggaa aaaatatctt attgtaaaaa atggacttaa
     6061 tggatttatg agacctatta tttctaattt aattgaaaat attacttgtg gagtagaatt
     6121 agaaggtcaa gagccattat atacatcaaa tcatgccttt tctctttcag tattaaataa
     6181 tttaaaagaa gaagagtatg taaatgttgt tgggaatctc tgtacatcat tagatgtaat
     6241 tgcaaataat gttaaattga aaaaagcaaa cataggagat ttgatttcaa ttagtaatgc
     6301 tggaagttac tcctattctt tatcaccgct attattttca agttatgaat ttcctaaaca
     6361 atattataaa agaaaagatg gatatattga gtaaacaata gcataaatta ataaacttag
     6421 tattattgaa aaaatttaaa atgcaggggg tgtaattcta tatggaattg tggaaaagaa
     6481 atttatttgt atgttggata ggtatgtttt ttagttctat agggatgagc caaatagcac
     6541 ctatacttcc attatatatt aaacagttgg gggtaactga tgttagttta atacaacagt
     6601 attcaggtat aatttttgga tgtacctttg tagtagcagc attcttttcg ccaatttgag
     6661 ggaaagctgc tgataaatat ggaagaaaat caatgctttt acgtgcaagt attgggatgg
     6721 gaatagttat atttaccatg gcgtttgttc aaaatgttta tcagttatta ggattaagaa
     6781 tacttcaagg agtttttacg ggttatgcta ctgcatgtac tactcttatt gcaacacaga
     6841 cagataaaaa tcattcagga tgggctttgg gtactcttgc aactgcatca actactggct
     6901 cattaattgg tccaacagta ggaggatata tagaatcaat cttaggtctt aaaagtacat
     6961 ttattataac aggaggattg ttgtttgtat catttatcat ctctatttta tttgtagtag
     7021 ataattttaa acctaaagaa ataaaagaat caatttctat acctaaagaa caattaaata
     7081 tattgccaaa taaattttta attgcatcta tttttgtaac cacattcatt actcaactag
     7141 ctttatactc tatagaacca attgtaacca tatatatatc acaacttaca aattttgcat
     7201 caaatgtagc tttaatagct ggattgacat tttctgcatc aggattagca aatttattag
     7261 cagctcaaaa acttggaaaa atgtcggata aaataggtcc tcaaaaagta ttgttaattt
     7321 cacttttatg ggctgggatt atatttatac cacaagcttt tgtaaaaact gcatggcaac
     7381 tgatgttgtt gagattttta ttagggttgt cagttgcagg gcttaatcct tctgttaaca
     7441 gcttgcttaa aaaaattgca ccagaagaat atgtgggaaa aatatttgga tataatgcat
     7501 ctgcccagta tataggctgt tcatcaggag catttttagg gggacaaata tcagcacatt
     7561 taggtattag aacagtattt ttttcaacaa gtttattgtt gtttaggaat gcactttgga
     7621 tgtataaatt tactggattg tttattaaag ataagactaa ataatcatac tatttacatt
     7681 tctaatttta tgatgtaaaa taaatatttt ttatgtaatt tatcatagaa ttatataaaa
     7741 aataacccta tggtaaaaat tggaaaatat tattatcgac aaaatctttg acttaaaaac
     7801 tatattgcga tagtattaat ttaataaaag tttgagaggg gcgtaaggtt atgacaattt
     7861 caaaaatacc acaagcagaa ttaaaagtta tgaagtttat ttgggaaaaa aatgatacag
     7921 taacatcaaa agaaactatt gaagctatgg aacgaaaata tggttggaaa caaacaacta
     7981 cattgacact tttatcaaga cttgttaaaa aacatttttt aagtgcagaa aaaatagata
     8041 gatacactca ttatacaata gttgttggac ataaggagta tttaagtgtt gagacaaaag
     8101 attttttaag caatatacac gacaattctt tacagagttt aatttcagca ctacatgatg
     8161 atgaagttgt tgataaagat atgttggatt tatttgaaag tcattttaaa aatttagaag
     8221 aagagtaatt atatcaggat agaactctaa gaatttaatt aatctatggc tgaaaaccat
     8281 aaatggtatt atttaatcag ctactcacta ttcataaagt aatattaaat aggttctcta
     8341 attagtaaaa tgcctaaaat aattggaata gggagtatca agttaaatga taccacttat
     8401 ttaaataaga aatgattttt aaaatttaaa ataattttat aacttgtcaa tattcattaa
     8461 tctaaagtat tatttctatt taaccatatc aaaatatatt ttactattt
//
LOCUS       DAMJVI010000131         8386 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.136, whole genome shotgun sequence.
ACCESSION   DAMJVI010000131 DAMJVI010000000
VERSION     DAMJVI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.136"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            94..849
                     /locus_tag="PC545_003253"
     CDS             94..849
                     /locus_tag="PC545_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2457991.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTNPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALHYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            980..2002
                     /locus_tag="PC545_003254"
     CDS             980..2002
                     /locus_tag="PC545_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2457992.1"
                     /translation="MINKKRLASLILAGALSISMLTGCSQGGDSGNSKQESNSKGKEV
                     KKIGITQLVEHPALDATRTGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILITAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNENISY
                     VKTKQE"
     gene            complement(2166..2723)
                     /locus_tag="PC545_003255"
     CDS             complement(2166..2723)
                     /locus_tag="PC545_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2457993.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEK
                     REDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2729..3805)
                     /locus_tag="PC545_003256"
     CDS             complement(2729..3805)
                     /locus_tag="PC545_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDF2457994.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISKLNRLDKNTIYATDWGCELTLNREIGDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIVENPFTYTIYVRKTDLSNECVPIQIELEKSKMRFKK
                     GDRIYLDFPQEYLFCFRYNYNKKE"
     gene            complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC545_003257"
     CDS             complement(3806..4489)
                     /gene="modB"
                     /locus_tag="PC545_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDF2457995.1"
                     /translation="MGTDWSPLWISLKTSILSTIITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLNFDRTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKIAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVAISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC545_003258"
     CDS             complement(4638..5450)
                     /gene="modA"
                     /locus_tag="PC545_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2457996.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNEDKKDDGKQEKTTKSSDSVE
                     LNISAAASLKEAMAKIEEEYKKVDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSSVSGMEDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSESGQKIFEEFGYKKVE"
     gene            5643..6572
                     /locus_tag="PC545_003259"
     CDS             5643..6572
                     /locus_tag="PC545_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2457997.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     TKDVEEYINSQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEITTWEDLTKPSITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGLGGYDLTDTGKTIAKV"
     gene            complement(6733..7182)
                     /locus_tag="PC545_003260"
     CDS             complement(6733..7182)
                     /locus_tag="PC545_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2457998.1"
                     /translation="MKNKNNKDKNMLNIKVLRVLGVLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGTDCIVFLLFIVECVYLVGRYILKVKINWSEIIENEKGNFINNLLVEKI
                     QEESNIVYNLLNSYVLPNIYKKARGLMFKTYHMLIYIKEKYYYVNIY"
     gene            complement(7385..8365)
                     /locus_tag="PC545_003261"
     CDS             complement(7385..8365)
                     /locus_tag="PC545_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2457999.1"
                     /translation="MKNSFKNCISLRNKFIIISIVFLLILLIVSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLEKLKNYKTSELMIQSSNNYKLESLFITSNIKTRDTMI
                     LVHGIGSSYYEMLKVAYRYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHSEINSKDDKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPDILINYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKDLWLIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
BASE COUNT         2918 a         1381 c         1012 g         3075 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agcttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ccgggcttat aaaagaatct tctggtcaag tattattaaa taaacataac
      301 ttgtctaatt tagctgaaca cagaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agtcttggaa cttgcccttc tatgactgtt agagaaaact tgtctttagc tcttaacaaa
      421 ggaaaactat taaatctaag aaaatgtctt cgttacaaaa gagatttctt agaaaattta
      481 ttggaaggtg tatctcttga tttaaaaaaa taccttgatg ttcaagtaaa atttttatca
      541 ggaggtcaaa gacaatcatt atcactaatt atgtcgtgct taacaaatcc atcagtttta
      601 ttacttgatg aacatacagc agcacttgac ccaaaaacat caaatgaagt aattgaattg
      661 actaataaga ttgtaaggga aaaaaatata actacactta tggttactca caacttaaaa
      721 cacgctcttc attatggtga tagattgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaaggta aagaaaaaga acaattaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtttaaa ataatatatc tattgtatta aactcggctt taaaataaag ttaaaattac
      901 aataattatg gttactttgt aataattcat atgaatatat gaacatatat aataataaat
      961 ttttgagggg gatataaaaa tgattaataa aaaaagattg gcaagtttga ttttagctgg
     1021 tgctcttagt atatctatgc ttacagggtg ttcacaagga ggagactctg gtaattcaaa
     1081 acaagaaagt aattctaaag gtaaagaagt taaaaaaatt ggtataactc aactggttga
     1141 acatccagca ttagatgcca ctaggacagg ttttgtaaaa gcattagaaa agaacggatt
     1201 taaagatgga gaaaatattg atatagactt ccaaaatgct caaaatgaca tgcctactac
     1261 acaaagtatt gcgagtaaat ttgcatctga taagaaggac ttaatatttg ctatatctac
     1321 tccatcagct caggcagcat ttaatgctac taaagatata cctatactta ttactgctgt
     1381 gagtgaccct gttgctgctg gattagttaa aactcttgaa aagccaggca caaatgtatc
     1441 tggaacatca gattttgttt ctgtagacaa aggtttagaa cttcttaaaa tatttgctcc
     1501 taaggcaaaa actataggag ttatgtacaa tactagtgaa gtaaattcca aagtccaagt
     1561 tgatgcttta aaagaatatg catctaaaaa tggatttaaa gttgttgaaa aaggtataac
     1621 tacttcaaat gaagttaacc aaggtatttc tagtttagtt ggcaaaatag acgtattata
     1681 tgttcctaca gataacttag tggcttcttc tatgcctata gtctctaaaa tagctactga
     1741 gaataaaata cctgttatag cagctgaatc tgggccagtt gaaaaaggtg ctcttgcttg
     1801 tcaaggtatc aactacgaaa aactaggtta caaaacaggt gaaatggcag ttaaaatttt
     1861 aaatggtgaa tcagtatctg atatgcctgt ggctacatca gatgatacag atatcattgt
     1921 taatgaagat atattaaaag ctttaggtat ggaaaaacct tctaatgaaa atatttctta
     1981 tgtaaagact aaacaagaat agaaagatat tttctatata cgattaatat gcctgtttct
     2041 ataaattaat ttatagaaac aggcatatat ttttatttta atgtattatc tatttaaaga
     2101 ttatatgtaa atgctcttaa tttgtttgta taaattttac ttgtataaac tttatacaat
     2161 catatttatt cttctatttt ctttattact ctacatggat ttccaacagc tacagtattt
     2221 ggtggtatat cttttgtgac tacacttcct gcacctatta ctacattatc acctatagta
     2281 attcctggtg ttatgattac tcccccacct atccatacat tatcacctat ttttacagga
     2341 gaaccatact ctatacctga atttctaagt tgtgcatcaa ttggatgata ggctgtatag
     2401 atttgaacgt ttggagctaa cattacattg tcacctattt caattttaca tacatctaaa
     2461 aaaatacaat cataatttgc aaagaaattt tcgcctacat gtatgttata tccataatca
     2521 cacctaatat tttgttctac atttatttgc ttgccaactg aaccaaatag ttgtcttaaa
     2581 atatcttctc ttttttcata ttcatcctct aaagtattat taaacaatct agttaatttt
     2641 ttacaatact ctctttcttt tactaacaat tcatcatttg catagtatcc tttcccagac
     2701 aacatttttt ctttttcagt catatagttt actccttttt gttataattg tatctaaagc
     2761 aaaataaata ttcctgtgga aaatctaaat atattctgtc acctttctta aatctcattt
     2821 tacttttttc caattctatt tgaataggta cacattcgtt agataaatca gtttttctaa
     2881 catatatagt atatgtaaaa ggattttcta caatgttaat caattcgaaa cataactttt
     2941 catttaaatc ttcattagaa tccaagacct taatatgatg ctccctgatt cccacatatt
     3001 ctatattatc acctatttct ctatttaatg tcaactcgca tccccagtct gtagcataaa
     3061 tagtattttt atccagtcta tttaatttgg atatattttt acaccctgtt atcttagctt
     3121 ctataaggta ctttggagat tcaaagagct cttttttagg tctctttgga agaccttctc
     3181 ctttgttgta cactataata tcatcacaaa ctcggtaagc ttcttcaata tcatgagtaa
     3241 caaatagtat atctcccttg taatctttta acatattcat aagttctttt tccatattgc
     3301 ttcttaaatg atggtccaat gctgagaaag gttcatctaa aagtaatata tctggagatg
     3361 ttgccaatgc tctagctagt gctacccttt gttgttgacc accagataat tgccaagggt
     3421 atctcccctc aaacccattt aatttaagta tatctaagta ccttgctact atttcctttt
     3481 tttcactctt acttagtttt aaaagcccaa gttctatatt ttgacttaca gtcatatgtg
     3541 gaaacaaagc atagttttga aataaaaatc ctacctttct atcttgtgtt gataaattta
     3601 tcttcttttc agaatcaaat agaactctat catttaatac aattttacct cttgttggtt
     3661 tctcaagccc tgctatacat tttaatgtca tactttttcc agaaccagat tctcccaaaa
     3721 accccagagt acctttttct tgtttaattt ctacctttag tttaaaagag gataaatctt
     3781 tttcaatatc cacgtataag ctcaactatc cacacctctt tcctataatt ttctgctgaa
     3841 attccgacca ataatttagt aataaaatca ttgctataga tattgctaca ataatcataa
     3901 cccacatcat agctttattc atatctccgc cttcaacagc aaaaaatata gctattggca
     3961 tagtttgagt ttttccaggt atatttccag ctatcataag tgtagcacca aattctccaa
     4021 gagctctggc aaacgacagc accaatccac caattattcc aggccatgct aatggaactg
     4081 caatcttaaa aaatactttc cattcattaa gacctaatgt tctagcagct gacaacatat
     4141 tattatcaat ctgttcaaaa gctgaccttg aagttctata catcatagga aaagacacca
     4201 ctactgctga aattacagtt gctgtccaac taaatattaa agtcctatcg aaatttaata
     4261 aaaactttcc tataaatcca ttcttcccac atagtaataa taaaaagaaa cctacaactg
     4321 ttggtggcaa aattagtgga agtgtaaata gtccatctat aagacccttc cacttacctc
     4381 tatagttagc cataatatat gacacaaata ttcctattac aaaagttatt attgttgaca
     4441 aaatagatgt cttaagtgat atccataagg gtgaccaatc tgttcccatc tttccctcct
     4501 taattttaaa tggaaatagt gaaactaata actctattaa tttcactatt tccattttat
     4561 tataatacac aattattttc aattttcatt tatttatata taatttatac ttaattacac
     4621 ataatttatt tctaagttta ttctactttt ttatatccaa attcttcaaa aatcttttgt
     4681 cctgactcac ttaataaaaa ttcttcaaat ttcttagcag catctacgtt tttactagct
     4741 ttaattactg aaactggata tgtaataggc gaatgagttt tttcgtctgt tttttcaact
     4801 acttttatct tatcattatt gacagtatca ctgaagtaaa cgaatccaac atcagcattt
     4861 cctgattgca cccatgctaa aacttctttt acatcttttg caaatactag cttatctttt
     4921 aacttatctt ttaaatttaa attagttaac acctcatctg catacttacc agctggaaca
     4981 ctttcagctt caccaacagc aattttcttc actttatctg tagtcaaatc ttccatacct
     5041 gatacactac tgtcagcaga agaaataaga actaaatcat tttttactaa atctttcata
     5101 gtatcagaaa ctaataactt ctcttcatcc aaagctttca tttgtttttg acctgctgaa
     5161 ataaataaat cacaaggtgc tccttgttct atttgttgtt gtaatgagcc tgatgctcca
     5221 tagttaactg taagttttac atttgaatca acttttttat attcttcttc aatctttgcc
     5281 attgcttctt ttaaacttgc agcagccgat atatttaact ctactgaatc actactttta
     5341 gttgtttttt cttgttttcc atcatctttt ttgtcttcat tactattgca cccaactgtc
     5401 cccaaagtta aacatgcaac taatcctaat attcccaata tttttttcat aacaatctcc
     5461 ctttttgtaa atttgataca cattttaaca tgttttatta tgttttatca tgttttgtta
     5521 tatatagttt aattatatat acatgataat aaattttcaa gtaatttatt tacactactt
     5581 ttaaatattt tgctacattt tccttttttt aatgttaaaa taaccttata tggaggtgga
     5641 ttatggactc attatcatct ttgacacctg tcgaggtagc tgaattatta aagataacaa
     5701 aaaatactgt atatgaatta atcaaaagag gtgaacttcc ttcttataaa gtagggaaaa
     5761 aaataagagt tgatactaaa gatgttgaag aatatatcaa cagccaaaaa actggtaaag
     5821 taaaggatac acatttcaac aataaaaata tagatataaa taataatttc aaaattatta
     5881 tttctggtca agatgtaata ttagacattt tagctaggtc tgttgaaaaa aaattggatg
     5941 gaataagtac atttcgctct tatataggta gttataatgc tttgtatgag ttatataatg
     6001 gcagagtttc tatagcatca tcccatctat gggatttcga aacggatgaa tataatagca
     6061 cttttgttaa aaagctattg ccaggaatac catgtgtact tataaatcta gcttatagaa
     6121 tgcaaggttt ctatgttgct aaaggaaatc ctaaagagat aacaacctgg gaggatttaa
     6181 caaaaccaag tataaccatt gtaaataggg aaaaaggttc tggaactagg gttttactag
     6241 atggtaaact tagacttcta aactttaatg gtaaacatat aaaaggttat gataatgaag
     6301 aattatcaca tcttggagtt gcaagtactg tttctcgtgg aatagcagat gttggtttgg
     6361 gaaatgaaaa agctgcttta caagtaaata atatcgactt tattcctctt cacaaagagc
     6421 gctatgattt agttattaaa aaagaagact tacaaaatcc agtttaccaa actataatta
     6481 acataataaa ctctcctgag tttaaagctg aacttcaagg tcttggcgga tatgatttaa
     6541 ctgatactgg aaaaactata gcaaaagttt aagattaaaa tcgcaatcta ttttataaga
     6601 tttataattt ttcacaaaat ttataactta aacacgaaat aaattgcgat tttagtgtct
     6661 caaaaaattt atgtctttca ttttatcatc caatacaaaa tataatataa ttaattgtgc
     6721 aatcatatta tattaatata tatttacata atagtatttt tctttaatgt agattaacat
     6781 atgatatgtt ttaaacatca atccccttgc tttcttatat atatttggca acacatatga
     6841 gtttaataaa ttatatacaa tgttgctttc ttcttgtatt ttttcaacca acaagttatt
     6901 tataaaatta cctttctcat tttcaataat ctcactccaa tttatcttaa cttttaatat
     6961 atatcttccc actaggtata cacactctac aataaataat aaaaatacaa tgcagtcagt
     7021 acctgttgtc ttatataaat tacccgatac taatggcttt gcgaagaaat tataaaaatt
     7081 caaagtatca cccgtataat caaaaagtat taatagcact cctagaactc tcaatacttt
     7141 aatattcaac atgtttttat ctttattatt tttgtttttc atatatacta cctctctttt
     7201 ctttagattt gattgtaatc taatactaac tcttttattt gaggtataaa tgaatttaat
     7261 tggtagttat tttcattttt actagaaatt aattcacatt actaaacaaa acttatattt
     7321 aaacataaaa atagcctatc tatcttttaa taaatacaaa aataaataag ctactaatat
     7381 tcaattaact tttatcactt aaaacattac tatctataaa tttaaatatt ttattgaagt
     7441 atactgtact atcatgttca aatccatcaa catgaccaat accttctata agccataggt
     7501 cttttttatc atgtttaact aaatcataca ttttcttgct attttgataa ttacaaacag
     7561 tatcttttgt tccatgtata aacagtatgg gtacattaga tttctcaact gactcatatg
     7621 gttttacatc actgtaccaa aatccagatt taaacttatt atataaatct ccacaagtca
     7681 ccacataatt gattaatata tctggtattc gtttttctag tactcccatt ctaattgcat
     7741 ctttcatttc agaataagga gaatccagta tataaaaatc gaccttatca tcttttgaat
     7801 ttatctctga atgcatagca gctgtccctg cccccatgga aaatccatgc acacctaatc
     7861 tcccttcagg aaatttattt tttacaaatt taactagact atctaaatca tacctttcat
     7921 aaagaccaaa tgtgtaatta tctccaccac tattaccagt atttctttga ttataaacta
     7981 atacattata acctttatct aaatatctat atgctacctt taacatttca tagtaactac
     8041 ttccaattcc atgaacaagt atcatagtat ctcttgtctt tatatttgaa gttataaaaa
     8101 gactttcaag cttgtaatta ttagaacttt gtatcattaa ttcagatgtc ttgtagtttt
     8161 ttaatttctc cagtggttta tcttttctct cagaaaatat ttttactata tcttcgtttt
     8221 ttattaactg ctctaaacca actgaactat tatacactaa ttttccaaat atataagata
     8281 caattaatag gattaataga aacacaattg atataattat aaatttattt cttaaactaa
     8341 tacaattttt aaaactattt ttcatattta tttaccatac aatttt
//
LOCUS       DAMJVI010000132         8329 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.137, whole genome shotgun sequence.
ACCESSION   DAMJVI010000132 DAMJVI010000000
VERSION     DAMJVI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.137"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(71..541)
                     /locus_tag="PC545_003262"
     CDS             complement(71..541)
                     /locus_tag="PC545_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2458000.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYKELFEQEIELDLGKNVTFKGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDDFDEFIEKLNYYKDVEFVHKPLKHEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLSEGYSVEETSKIIQHPIKFVKQCENSKE"
     gene            complement(1275..1664)
                     /locus_tag="PC545_003263"
     CDS             complement(1275..1664)
                     /locus_tag="PC545_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2458001.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYQWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEKDV"
     gene            complement(3463..4869)
                     /locus_tag="PC545_003264"
     CDS             complement(3463..4869)
                     /locus_tag="PC545_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2458002.1"
                     /translation="MDSVKYILRRFIGSSILISLLFIVLNIAGFLLFSYMLFVSKYSD
                     NLDTSDSREIKSNLIMIEDNLILRNGKFILDDSSKEVLHKNKVWAMLIDNNGDRIWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEHPNGILVTGYPKDKYTKFNLTYTVKE
                     IEQFPMLIIGMFLVNAIVTFLIALFIGLKMVKSIKPIITGIKLMSKGEPVLLQEKGIL
                     SDISKSINTVSKELQMKDEKLRKKEEARSNWIAGISHDIRTPLSMIIGYAGELEESSS
                     LSNREQEQVSIICNQGIKIRELVNDLNLVSKLEYNMQALNCDKIRVSAFLRELISEFI
                     NNNLDNDFYVEFDILNEDIIIDADKKLLKRAISNLIQNSITHNQQGCNIRVTSKSDLD
                     FCYIIVEDDGKGMSQERINSLLKSQNGLSEAYAKGQNHGLGILIVSGIINAHRGKLDI
                     TSIDSQGLKTILKLPLIK"
     gene            complement(4914..5621)
                     /locus_tag="PC545_003265"
     CDS             complement(4914..5621)
                     /locus_tag="PC545_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458003.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRVFLASSYEEAV
                     RVFRNVKPDCAILDVVLPDGDGFSIMRKIRETSKIPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVGILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTNDSLCQAIWGDDMYGYENTLMVHVRRVREKIEKTPST
                     PKHLITVRGLGYKLMIN"
     gene            5792..6706
                     /locus_tag="PC545_003266"
     CDS             5792..6706
                     /locus_tag="PC545_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458004.1"
                     /translation="MEMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKS
                     TTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIM
                     QKLLDLPKKNVNEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPT
                     NGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIK
                     ELHNRGQSQIAIKTKNVVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINGMLVHS
                     NIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            6706..7467
                     /locus_tag="PC545_003267"
     CDS             6706..7467
                     /locus_tag="PC545_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458005.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAITRNPD
                     MLGGFEYSYMFMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVTSLNSIYL
                     SKFLCSAILVSIPVLMQVLSIVLFGHFRGVIESISVSLLLKYTLGTILVSIAIIALHL
                     WIATVIPNQMIAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDMNFSITILVFLIGMLIYYIGRNHLLKKEV"
     gene            7479..8216
                     /locus_tag="PC545_003268"
     CDS             7479..8216
                     /locus_tag="PC545_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458006.1"
                     /translation="MLRKSVLGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSILTAITQVFFVIFYIVAGNMFGFSGSVPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVAKPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     TICVAFITTIVFSEIANRNFRKNDVIS"
BASE COUNT         2787 a         1360 c          870 g         3312 t
ORIGIN      
        1 tagtcctata tattatcttg aattagttct attacaatta atttctacta aataattata
       61 atttaaacaa tcattcctta ctattttcac actgctttac aaatttaatt ggatgttgaa
      121 ttattttaga agtttcttct acagagtatc cctcagataa atatcttcta gctataactt
      181 ccatagactc tcctatctca ataatatgct tatccgggtc ataaatacgc actactcttt
      241 gttgccattc atgttttagt ggcttatgga caaactctac atctttataa taattgagtt
      301 tttcaataaa ttcgtcaaaa tcatcaacct caaaataaag ttccatatta tttgattttt
      361 gtataattgt atctggatta atccctgtaa gccatgcgaa gtcttcttgt atagcaaatc
      421 ctcctttaaa tgttacattt ttacctaagt ccaattcaat ttcttgctca aataattctt
      481 tgtaaaattt ttttgatact tcaatatcct tcactgctaa taatgaaacc tgatatttca
      541 taaattttcc tcctaatttt ttatttctat tttttcagta tcttctatta acattttttt
      601 ctctatctct tcagtacctt ttattaatcc tttattttta cttttttagt atcttctatt
      661 aacacttttg tttctacctc ttcagtatct tatatcaatc ttttattttt tcaatatctt
      721 ctattaacac ttttatctct atctattcag tactttctat caatcttttg tttttatttt
      781 ttcaatatct tctattaata cttttgtttc tacctcttca gtatcttata tcaatctttt
      841 attttttcaa tatcttctgt taacactttt atctctatct ctttaatatc ttatattaat
      901 cctttatttt tattttttca atatcatcat taattcctat ttattttatc atcttaaata
      961 cgaaaacact gtgtcatatt tgcaaaatat atattatcat ctatattaaa gcttcacaat
     1021 ttatatttaa aaattctctt taattacact acctctattt aaaaagctac cttaattata
     1081 cttataagta aaggtagctt tttaaattta attaaataga atatccacta ttaattttta
     1141 tttaatttat ttagattcac attatagtat cataatccac ttataatcat ttgtcatatt
     1201 acttaagtag actaatatat tacataaata cacttacata cactttatat tgatgctata
     1261 agaaatacgt ttgcttatac gtctttctcc tcttctttta gattttttat ccactcttca
     1321 ataagtaaga tatcatcttt gctcaaatta atattttcat gtaatgacaa caataaactt
     1381 tttatagaag aatcatgaat atttctaaaa aaatctcttg tctcaactcc tatgtattcc
     1441 ttttctttaa ttaaaacttc ataatgcgta tatttatcta tcttttgaga atttagaaat
     1501 ctttttacta ctaatctaga taaaagagtc aaagttgtcg tttgtttcca ttgatatttt
     1561 tgctccattc ctaaaacaat atctctagat gttactttgg aatctgattt ccatataaat
     1621 ttcattatct ttaattctga ttgtggcaac ttttttatag tcatgataaa accttgctac
     1681 tcagaatata gacaagcaat atccataata aaatttttcg attaaataca tcttatacgg
     1741 atttatataa gctcagactt ataagatatt tttcaatctt aatcctaaat ttagctattt
     1801 gtacacggta aatatatata aataatgtca aaattctctt gtattatatt gcttaatttt
     1861 atgaaatatt ttgtactcag ctatctattt tggatacttg tccagtagct ctcctttctt
     1921 ttaaactctt tgattttaaa tatttgtatt tcagtaaatc ctacttccta aattttttat
     1981 ttactacaac ttattactga acttcctact tcctaaactt tttatttact acaacttatt
     2041 actgaacttc tcacttacta aatttcttac ttactataaa ttataactaa acttctcact
     2101 tactaaattt cttacttact ataaattact actaaacttc ctactttcta aatttcttac
     2161 ttgctataaa ttattactaa acttcctact ttctaaattt cttacttgct ataaattatt
     2221 actaaacttc ctactcccta aatttcttac ttgatacaaa ttattactaa acttcctact
     2281 ccctaaattt cttacttgct ataaattatt actaaacttc ctactcccta aatttcttac
     2341 ttgctataaa ttattactaa acttcctact ttctaaattt cttacttgct acaaattatt
     2401 actaaacttc ctacttccta aatttcttac ttgctataaa ttattactac taaacttccc
     2461 acttcctaag tttcttactt gctacaaatt attactaaac ttcctacttc ctaaatttct
     2521 tacttactac tacttataac taaacttccc acttcctaaa tttcttactt actactactt
     2581 ataactaaac ttcccacttc ctaaatttct tacttactac cacttataac taaacttccc
     2641 acttcctaaa tttcttgctt actaccactt ataactaaac tttttacttc ttaaatttct
     2701 tgcttactac cacttataac taaacttttt acttcttaaa tttcttactt cttaagaata
     2761 atgttaattt taagattata gtttttgcct tgtttaattg aaacattcta tataattata
     2821 tccttctatt ttagagtttt tttatttata tattacaaag tttaaatttt ttcattaatt
     2881 atattataaa tttcccatat atttcaacat aaatacatat atatatatgt tatttctaaa
     2941 aatactacac aaaaactcta ctttagtaga actcaatggc gcaaaatact tcaatttaaa
     3001 acatttatcg tactttaaat aaataactat aataatatac ttacaatagt tacatagaag
     3061 ctattataac tacacagaac agcgtataat taaaactttt ttaggatgtc attatatttt
     3121 ttcttaaaat taaaaaacac tataccataa ttctatttta gtagaatttt tttgtcaata
     3181 ataaaattaa atattaacta aaaaataaaa aaaatggaat tatctaaaga atttaataac
     3241 cttacaataa ttccaaaata ttatttctat gaaaattttg ataccatgag taactcagcc
     3301 ataagcgcat aaagcacaag ttactcttta ctttaataaa acttgacaag aaaagaattt
     3361 atccttattc tactctaata gagttaattt gtcaataaag aataaaatta ttttcaaaat
     3421 attttcattc ttcattctta tctgttatta ttttataaaa tatcacttaa ttagaggtaa
     3481 ttttagtatt gtttttagtc cctgactatc aatgctcgta atatctaatt tacctctatg
     3541 tgcattaata atgccagata ctatcaaaat acctagacca tgattttgac ctttggcata
     3601 tgcctcactt agtccatttt ggctctttaa taaactatta attctttctt gactcatacc
     3661 ttttccatca tcttcaacaa ttatatagca aaaatctaaa tcagacttag aggtaaccct
     3721 tatattacat ccttgttggt tatgagttat actattttgt attaaattac taatagccct
     3781 ttttaataat tttttatctg catctataat aatatcttca tttagtatat caaattcaac
     3841 gtaaaagtca ttatccaaat tattatttat aaactcagaa ataagctctc tcaaaaacgc
     3901 agaaactcta atcttatcac aattcaatgc ttgcatatta tattcaagtt tagacactaa
     3961 atttaggtca tttactaatt ctctgatttt aatcccttga ttgcatataa tagacacttg
     4021 ctcttgttct ctatttgaaa ggctactgct ttcttcaagt tctccagcat atccaattat
     4081 cattgaaagt ggagttctta tatcatgaga tattcctgca atccagttag acctagcttc
     4141 ctctttcttc ctaagttttt catctttcat ttgcaactct ttagatacag tattaataga
     4201 ttttgaaata tcacttagta ttcctttttc ttgtaaaagt actggttctc cttttgacat
     4261 aagctttatg ccagtaataa ttggttttat agatttcacc atttttaaac ctatgaatag
     4321 agctattaaa aaagtaacta ttgcattaac caaaaacata cctattatta acataggaaa
     4381 ttgttctatt tccttaaccg tataagtaag gttaaactta gtgtacttgt cttttggata
     4441 acctgttact aatattccat ttggatgttc ccatacatac acaggatagt cctttaaaaa
     4501 gaaccttgaa aactttgcta catctgtcaa agtataatga cttggaattt cttttggtaa
     4561 atcaaatccc catattctat ctccattgtt atcaattaac attgcccaaa ctttattttt
     4621 atgtagtact tccttactag aatcatctaa aataaacttt ccatttctta aaatcaaatt
     4681 atcttcaatc ataatcaagt ttgattttat ttctctgcta tcacttgtat ctaaattatc
     4741 agagtattta gatacaaata gcatatatga aaacagaaga aaacctgcta tatttaatac
     4801 aataaaaagt aatgaaatca agatacttga acctataaat cgcctcaata tgtactttac
     4861 actatccaaa attaaatcac tcctcttgat ttcttttatt ctaatatcta aaattaatta
     4921 atcatcaact tatatccaag acctcttact gtaataagat gtttaggtgt agatggagtt
     4981 ttttctatct tctctctaac ccttctaaca tgaaccataa gggtattttc atatccatac
     5041 atatcatctc cccaaatggc ttgacatagt gaatcattag taacaattct ccctttattt
     5101 tcatacaact ttaaaagcaa cgaatgctct ttagctgtta aagaaatctc ttgcccatct
     5161 ttctcaacac atgcactatt taaattcaca acaacagaat ctcccagttt aaatactggc
     5221 aactcctctt cttctatagg cacatataca cgatttaata tacccacaag ccttaaagta
     5281 agttcctttg gtaaaaatgg ctttactata taatcgtcag cccctaagcc caaaccaatc
     5341 aatctatctt catcttcacc tcttgcagag agaaaaatca ctggtatttt agatgtctct
     5401 ctaatttttc tcataataga aaaaccatct ccatctggca atacaacatc taaaattgca
     5461 caatctggct ttacatttct aaatacccta acagcttcct cataacttga tgctaaaaat
     5521 actctactaa acccctcttt tctcaggatt tcatctatca tttttaataa ttccttgtgg
     5581 tcatcaacca caagtatttt tttatctttt attgactcca taatcatcac cctcctttag
     5641 tataccatat acccattttc acaactttgc ctaaatagac ttttaaggtt attgtaaggt
     5701 aaatttcagc taattgaaat gtaaccaagt tattatactg atataagtta aaagaatcaa
     5761 taagcacaga ttaaaaaagg ggatgaaaca tatggaaatg ataattagaa cacaaagact
     5821 ttcaaaggaa tacaataaaa tatttagagt aaaggatata gacctaagag ttccacaagg
     5881 agctgtatat ggttttttgg gtcctaatgg agctggaaaa tctaccactc ttaaaatgct
     5941 tcttggactt gctaaaccca cacaaggaaa tattaatatt ttaggtaaag agttaaatga
     6001 aaaaaataga atatcaattt taaaagatat aggttctttg atagaatctc catcatacta
     6061 tggtcatctt acagggcttg aaaacatgac cataatgcaa aaactcttag atttacctaa
     6121 aaaaaatgta aatgaagcat taaaaattgt aaggttagaa aatcaaaaaa ataaaaaagt
     6181 tagtcaatat tctttaggta tgaagcaacg tcttggtatt gctatggcaa taatgaagtt
     6241 tcccaaatta ttaatacttg atgaacctac aaatggactc gacccagcag ggattgagga
     6301 aattagagaa ttaataaaat ctctaccaga aaaatacggg atgactgttc taatctctag
     6361 ccatctttta agtgaaattg accaaattgc cacatcaata ggaataataa atcatggcga
     6421 actggttttt caagatagca taaaagaact tcataataga ggtcaaagtc agattgctat
     6481 caaaactaag aatgttgtat ctgcacaaaa actattaaat actaaaggat atatgtcact
     6541 tatacaagat gggtacttga ctattgaaaa tttaaaagac tcagatgtgg ctaaaataaa
     6601 tggtatgctt gtccattcaa atatagatgt atttagaata gaagaacata aaaaaagctt
     6661 ggaagatata ttcttagaac ttactggtaa ggcggtgact ttataatgaa ataccttggt
     6721 ctagaatttt ataaacttaa aagaaagaag attattctga tgaccttttt atttgtagct
     6781 gttgaaataa tgtggtgtat cataaattca aatagagcta ttacacgtaa tccagatatg
     6841 ttaggtggat ttgaatattc ctatatgttt atgagttttg caagtttaaa tggtctattc
     6901 tttcctatct taatatcaat aattacatca cgtataagcg atatagaaca taaaggagat
     6961 acatggaagc ttttaaaatc ttctgtgaca tctttaaata gtatttattt atctaaattt
     7021 ttatgttctg ctattttggt atctatacct gtattgatgc aagtgttatc tattgtgttg
     7081 tttggacatt ttagaggggt tattgagtca atttctgtat ctttactttt aaaatacaca
     7141 ttaggaacaa ttcttgtaag tattgctatc attgcactac acctatggat tgcaactgta
     7201 attccaaatc aaatgattgc aattacattt ggcatgattg gtagttttat aggtttgaca
     7261 tcaggtttgt tttatcaagg tattcgtaga ttgtttatat ggtcttatta cctagagtta
     7321 tcacctttaa gttatgctta tgataatgtt ttaggttcat ctgtctatag aattgatatg
     7381 aatttttcta ttacgatttt agttttctta attggaatgc taatttatta tattggaaga
     7441 aatcatttat taaaaaaaga agtataggag ggatttaaat gttaaggaag tcagttttgg
     7501 gagaaattct aaagttaaaa aattctttta tatggtatgt attattagct ttgccactta
     7561 taagtgtgtt gattggttct ggtaattttt atctaaatca aggcatactc aaaaaagaat
     7621 ggtacagctt atggactcaa gttagtcttt tttatgggga atttttcttt cctatcttaa
     7681 tcgcaatctt ttgtgcatat atttgtaggc ttgaacatat gaatcataat tggaataatg
     7741 taatgacact gccagttaat ttaaaaaata tctttttatc taaacttaca gtagtttcaa
     7801 tacttacagc tattacacaa gttttctttg ttatctttta tatagttgca ggaaatatgt
     7861 ttggtttttc tggtagtgta cctaaagaat tgactggttg gattgctaaa ggttggatta
     7921 tttctatttc aatagcttct atacaattat acatgtctat aaagattaga agttttgcaa
     7981 ctcctattgg tattagtctt tgcctttctc tatttggtat gggattaata gtagcaaaac
     8041 ctgctgttgg tatatttttc ccttattcta tgttaggtag tgctatgggg gttataaagc
     8101 aatctgcttt aaatgttaat gacactatag ttacaatatg tgttgcattt attactacta
     8161 tagtattttc tgaaattgcc aatagaaatt ttagaaagaa tgatgttatt tcttaatggt
     8221 aaatgctaga tattttgggg tttgaaattt atattctttt tagttgaaat ctatatccat
     8281 tttatttaaa aatatatatc ttttatttga attttatatg ttttatcta
//
LOCUS       DAMJVI010000133         8197 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.138, whole genome shotgun sequence.
ACCESSION   DAMJVI010000133 DAMJVI010000000
VERSION     DAMJVI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8197
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.138"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            32..1915
                     /locus_tag="PC545_003269"
     CDS             32..1915
                     /locus_tag="PC545_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDF2458007.1"
                     /translation="MSNKNKENITLSNQIKGTIQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIVMSKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQEEKLLFTEKRIIELGNENDEKELCEIGNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDPSINPEGVDKWGAIPTRDNFTGGDLQGV
                     IEHLDYLSDLGINGIYFCPITIGKTNHRYDTVDYMEVDPTLGDKETLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKNWFYIKDMSKIDTPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIKYLLDVGKYWIQEFDIDAWRLDVSNEVDHVFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVSEVIFNLLGSHDTTRILTYANGNIDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNKDIFDFMKKIIRLRKENKELRSISNEWVLADKE
                     NGTIILKKESISIIINNSSKNIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
     gene            2140..2820
                     /gene="pgmB"
                     /locus_tag="PC545_003270"
     CDS             2140..2820
                     /gene="pgmB"
                     /locus_tag="PC545_003270"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDF2458008.1"
                     /translation="MIEAFIFDLDGVITDTAYYHYMAWRKLAHKVGIDIDTKFNESLK
                     GISRMESLDRILEFGNKKYSFSEEEKVRMAEEKNNYYVSLIDEITSNDILPGIESLLI
                     DVKSNNIKIGLSSASKNAINVLNHLGISDKFDFIADAGKCKNNKPHPEIFLMSAKGLN
                     VNPQNCIGIEDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYN
                     EYIVRRII"
     gene            2817..5102
                     /locus_tag="PC545_003271"
     CDS             2817..5102
                     /locus_tag="PC545_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDF2458009.1"
                     /translation="MKCLFSNVDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGVRIFIDDVELDL
                     AKCKVEDFYRELDMKNGILNRRFIVNINDKKFEVRVARFLSISVKELAVIKYSIKSLN
                     SNTKIKFVPYLDSNIKNEDSNYEEKFWNGISSSSLEYKGSILSRTKENAFNTPVFTVG
                     SMMYIKTNGDVEAVSHESDYNYCENSITMDVLKNEVASIEKYVSITSTRDYKESELLR
                     KCEDILNRELSKSYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     DNAKKLGLKGALYPMVTFDGVECHNEWEITFEEIHRNGSIVYAIYNYTNYTGDYKYIK
                     EKGIDVIIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNINNNWYTNYIAKWCLDY
                     AVENILRLEKECNECIDRNNVTREEVEFWKIISNKMYLPYDEELQIIIQNDNFLDKEF
                     VEVKDLPKENFPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWNETLSFDPKLPKEWKNYSFNINYRDNIINVDVCSDLIKIENLKGR
                     PIKIIVYGKKYILEKCIKIDK"
     gene            5327..6220
                     /locus_tag="PC545_003272"
     CDS             5327..6220
                     /locus_tag="PC545_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDF2458010.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREIKKDDINVSKYIKYAD
                     LASKNNAQVNWKYVASIVAVLNKNNLKNVKDSQIQEVSDLFVKNFSKNNKINKLSDIL
                     DELEFSNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYSNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKNNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
     gene            6657..7148
                     /locus_tag="PC545_003273"
     CDS             6657..7148
                     /locus_tag="PC545_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458011.1"
                     /translation="MYENNKVIYNLERCEKLEKTLYLSQFNTYKNFKLASKKTVINSL
                     ETYVYNMNINNISLVDTIECISLEDEQLTGKKLILVGDIDIDAILDYAGNKRNRNSSK
                     KNTFKLKIPFSTFIQMPRKIENKDKINLKYLIQDITSSILDDNLFISVTAIISYENSS
                     KIE"
     gene            7176..8123
                     /locus_tag="PC545_003274"
     CDS             7176..8123
                     /locus_tag="PC545_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458012.1"
                     /translation="MRITKTTFNDVFSSDANESIIKVEKYVDKKCASVFEILTYTIIV
                     TNTNRYKTGNIFFKDYISKYIEFINNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNLDKFPTRISIESNRVYTNVNKIVFKQLNISN
                     TLKAPKNLRDILKVNVNSKIFDVKPMKNLINKGSNLKLCNLIVFGCIEVEIDYSCKNR
                     SNFKTVNKSNDIKKDKYYKNDIQNNNENINDCKKQISFGDNKVKKITKTFGFSCFICS
                     PVGIIYEDMKNINIKIEHTSINELNPEELFVNTSLLLYY"
BASE COUNT         3465 a          723 c         1395 g         2614 t
ORIGIN      
        1 ataaattatt ataaaggagg gaaattttaa aatgtctaat aaaaataaag aaaatataac
       61 tttaagtaat caaatcaaag gaacaattca aaatagttta acaaaagaag ctatattaca
      121 tataccaatg agcaattatg cctatggtta tgatagagaa acactacata taagacttag
      181 aactaaaaaa aatgaagcga aaagagtaat tttacgaata ggagaccaat atgtgtggga
      241 taaaggaggt gcaggaggag gaaatttaaa tgcatctgga gttggttggt ctggtggaac
      301 aaatatagta atgagtaaag aagtagaaac tgaattattt gactattgga tagctaagtg
      361 taagccttta aataaacgct cacgatatgg ctttataata gaaggtcaag aggaaaaact
      421 tttatttaca gaaaaaagaa taatagagtt gggaaatgaa aatgatgaaa aggaattatg
      481 tgaaataggt aatttttttg gatatccata tttaaattat atagatatac ctaaagtgcc
      541 aaattgggtg aaagatactg tttggtatca gatatttcca gatagatttg caaatggtga
      601 cccgtctata aacccagaag gagtagataa atggggagct attccaacca gagataattt
      661 tacaggtgga gatttacaag gtgtgataga acacttagat tatttgagtg atttgggaat
      721 aaacggaatt tacttttgcc ctattacaat tggaaaaacc aatcatagat atgatacggt
      781 agattatatg gaggtagacc ctacattagg agataaagag actttaaaaa aactaataga
      841 agaagcgcat aagagaaata taaaaataat gttggatgct gtttttaatc atataggata
      901 ttattctaaa cagtggcaag atgtagttca aaataaggaa aattctcgat ataaaaactg
      961 gttttatata aaagatatga gtaaaattga tactccaata gaacaaatag atgaaaaaaa
     1021 tataccttat gaaacctttg ggtgtgaaaa atatatgcct aaacttaata cagaaaattc
     1081 agaagtaata aaatatttat tggatgtagg caagtactgg atacaggaat ttgatataga
     1141 tgcgtggaga ttagatgttt caaatgaagt agaccatgtt ttttggagaa agtttagaga
     1201 agaagtcaaa aaagtaaaac cagacattta tatattggga gaaatttggc atggtagttt
     1261 accatggcta atgggtgacc aatttgattc agttatgaat tacttgatgt cagaagccat
     1321 gaaaaaattc ttttgtacag atgagataaa cgccgaagaa tttaagtata tgataaatga
     1381 tgtaatggta agttatccaa tacaagttag tgaagttatt tttaatttac ttggaagtca
     1441 cgatactaca agaatattga cttatgctaa tggaaatatt gatagattta aacttgcata
     1501 tatgtttatg tttgtacaaa caggatgtcc ttgtatatac tatggtgatg aaatcggaat
     1561 ggaaggagaa ttaagttcaa tatctgaagg tcaaagaaaa tgtatggaat ggaatgaaga
     1621 aaaatggaat aaggatatat ttgattttat gaagaagatt attagacttc gtaaagaaaa
     1681 taaagaactt aggtctatat caaatgaatg ggtgttagca gataaggaaa atggaacgat
     1741 tatattgaaa aaagaatcta tttctataat aataaataat tcatctaaaa atataatttt
     1801 gacgttacca gattatttag caaataaaaa agtaaaagat ttatatgaag aagaaatagt
     1861 tgatttaaaa gaagaactaa tgttagagaa atacaagttc attattttaa aataatacaa
     1921 aaaagtattt attttagatt tcatgttaaa ttattataca gtttaaataa gtctttataa
     1981 acgataaaaa aatatggtct acaagtgagt gaattgaaag tagattttaa attaaagact
     2041 gagttagagt agataatttt ataaataata tcttaaatac aagatagaaa ataaattgat
     2101 tattagtcta aaaatttctt gtagaaagag gaaataagaa tgattgaagc atttatattt
     2161 gatttagatg gtgtaattac agatactgca tattatcact atatggcttg gagaaaacta
     2221 gcccataaag ttgggataga tatagacaca aaatttaatg aatctttaaa aggcataagt
     2281 agaatggagt cattggacag gatattagaa tttggaaata agaaatattc attttcagaa
     2341 gaagaaaaag tgagaatggc tgaagaaaaa aataattact atgtatcatt gatagatgag
     2401 ataacttcaa atgatatttt gccaggtata gaaagcttgt taattgatgt aaaatctaat
     2461 aatataaaga taggtctatc ttctgcaagt aagaatgcaa taaatgtatt aaatcatttg
     2521 ggtataagtg acaaatttga ttttatagct gatgcaggaa aatgtaaaaa caataaacca
     2581 catccagaga tatttcttat gtcagcgaaa gggcttaatg taaatccaca aaattgtata
     2641 gggatagagg atgcaagtgc aggtatagac gctataaact cagctaatat gttttctgtt
     2701 ggtgttggaa attatgaaaa cttaaaaaaa gcaaatttag ttgtagattc aacaaatcaa
     2761 ttaaagtttg aatatataca agaaaagtat aatgagtata tagttaggag aattatatga
     2821 aatgtttatt tagtaatgta gatgagtgga agattataca agataagatt gaaatcaaag
     2881 aaaatagatt agcagagtcc ataatgagta taggcaatgg atatatgggt atgagaggaa
     2941 actatgagga aaattatagt ggagatacac acaaggggtc ttatatagct ggagtatggt
     3001 ttccagataa aactcgtgtt ggttggtgga aaaatgggta tccagaatac tttggtaaaa
     3061 ttcctaattc agtcaactat ataggtgtaa gaattttcat agatgatgta gaattagatt
     3121 tagcaaaatg taaagtagaa gatttttata gagaattaga tatgaaaaat ggtattttaa
     3181 atcgtaggtt tattgtaaat ataaatgata aaaaatttga agttagggtt gctaggtttt
     3241 taagtatatc tgttaaggaa cttgcagtaa ttaaatacag tataaaatca ttaaattcta
     3301 atactaaaat aaaatttgta ccatatttag attcaaatat aaaaaatgaa gattcaaact
     3361 atgaggaaaa gttttggaat ggaatttctt caagttcttt agaatataaa ggtagtattc
     3421 tttctcgaac aaaggagaat gcatttaata caccagtatt cactgtgggt tcaatgatgt
     3481 atattaagac aaatggtgat gtagaagctg tatctcatga gagtgattat aattactgtg
     3541 aaaattcgat aacaatggat gttttaaaaa atgaggttgc aagtatagaa aaatatgtat
     3601 ctataacttc aactagagat tataaggaaa gtgagctatt aagaaaatgt gaagacattt
     3661 taaatagaga actatctaaa agttatgatg atatattaaa tgaacataca aagttatgga
     3721 ataaaagatg ggaaagtgca gatgtaaaaa taaatggaga taaaagtagc caacaaggta
     3781 taagatataa tttattccaa ctattttcaa catattatgg agaagattcg cgattaaata
     3841 taggtccaaa aggatttact ggtgaaaagt atggaggagc tacttattgg gatacagaag
     3901 cgttttgtgt tccagtatat ctaggtgtag cagataagga ggtaactaga aatcttttag
     3961 aatataggta caatcagtta gaacaggcaa aggataatgc aaaaaaacta gggcttaaag
     4021 gtgctttata tccaatggta acatttgatg gagtagaatg tcataatgaa tgggagataa
     4081 catttgagga aatccaccga aatggaagta tagtatatgc aatatataac tatacaaatt
     4141 atacaggtga ctataaatat ataaaagaaa aaggaataga tgtaataata gaaatagcta
     4201 gattttgggc aagtagagtt catttgtcta caaaaaaaga tttgtatatg atacatggag
     4261 taacaggtcc aaacgagtat gaaaacaata tcaataataa ttggtatact aattatatag
     4321 ctaaatggtg tttagattat gctgtagaaa atatcttacg attagagaaa gagtgtaatg
     4381 aatgtattga cagaaataat gtgactaggg aagaagttga gttttggaaa ataatatcaa
     4441 ataaaatgta tctgccgtat gatgaggaac tacaaataat aattcaaaat gataattttt
     4501 tagataaaga atttgtagaa gtaaaggatt taccaaaaga aaattttcct cttaatcaaa
     4561 attggtcttg ggataagatt cttaggtcgt gttttataaa acaagcagat gtattacagg
     4621 gaatttacta ttttggaaat agatttacta aagaagaaaa aaagagaaat tttgacttct
     4681 atgaaaaata tactgtacat gaatcttctt tatcttctag tatttattct attattgcct
     4741 cagaaataga taacctagaa aaggcttatg aattatattc aagaacagct aggcttgatt
     4801 tagataatta caataatgat acaaatgatg gactgcacat aacttcaatg tctggttcat
     4861 ggctttccat tgtacatgga tttgcaggca tgagaacatg gaatgaaact ctatcttttg
     4921 accctaagtt gccaaaagag tggaaaaatt attctttcaa tataaattat agagataata
     4981 ttattaatgt agatgtatgt agtgatttaa ttaaaataga aaatttaaaa ggtagaccaa
     5041 taaaaattat tgtatatggg aaaaaatata tattagaaaa atgtataaaa attgataaat
     5101 agatttaata atatttttat tatataaata gttaaaataa gctatctaat tatttattta
     5161 ttttaactat tttgtgtgat agaaaactat atattataac aatagtagtg cttttaatta
     5221 aatatatttc ataaatcttc ataaaatctt caaaatttcg taaagaatat gtcacaaaag
     5281 taaggtatat tatattcagc agatagtata agggaggaca atatttatga agaataaaaa
     5341 attcattgtt ataatactga taatatcaat tttaataggt gtattagtga aggaatattc
     5401 atctagagag ataaaaaagg atgatataaa tgtcagtaag tatataaaat atgctgattt
     5461 agcaagtaaa aataatgcac aagttaactg gaagtatgta gcttccattg ttgctgtgtt
     5521 aaataaaaat aacttaaaaa acgttaagga ttcacagata caagaagtat cagatttatt
     5581 tgttaagaat ttttctaaaa ataataaaat aaataagtta agtgatattt tggatgagtt
     5641 agaatttagt aatagacaaa aaagattggt tgataattat atagacaatc ttaaggatta
     5701 tggtataaaa ccagaaagat tgaagtctga tacaaaatat atgaagttta ttgatgaaat
     5761 taaaacagaa gcaatacaaa attataagga ttataaaata cttccatcta taactattgc
     5821 acaagctata atagaatcta gctggggcaa gtcaactttg gcaaaacaat atagcaatct
     5881 atttggtata aaggctgacg cttattggaa aggtaagtca gtgactttag aaacgaaaga
     5941 acatttagat acaattatag atgataaatt tagaatatat gatgataaaa atgaatctat
     6001 aaaagaccat gcaaaatttt tagccacaaa taaaaggtat aaaaacaatg gtgtatttga
     6061 tgcaaaaacg tatatatatc aagctaaagc attagaaaag gcaggttata gtactgcgaa
     6121 ggatgaaaat ggcaatagta tatatgcagc tcgtctaata gaattaattc aacagtataa
     6181 tcttcaatta atagacagtg aaattcaaag tgaaatgtaa ctttgattaa atttttattg
     6241 ataaggctat gatttttaaa atcatagcct tattttgaca tgcttttact tgtaattatt
     6301 aaatcgatat cattatcttt caaaatttac tttatactgt aaatcaaatt atttaagtta
     6361 aaacttggaa ttatttataa aaggattatt tactttatct aaattctgca cttaaagaac
     6421 atcttttccc ttaagactga ccgttagtct ataaaaatta taattactta taaaaaagtg
     6481 aatatatata taaagttttc agaaagactt ttacttgtaa attgaaatca atgaagtaaa
     6541 aaaggattct ttcttaaaag taaataacaa ctagatagtg aataataaaa aatggtaatt
     6601 tgaaataatt tttatgaata gtattaatat atagatacag ggagattgat gcgattatgt
     6661 atgaaaataa taaggtaata tacaaccttg aaagatgtga aaaactagaa aagactcttt
     6721 atttatctca atttaataca tataaaaatt ttaaattagc ttctaaaaaa actgtaataa
     6781 attcattaga gacatatgtt tacaatatga atattaacaa tataagtctt gttgatacta
     6841 tagaatgtat atcattagaa gatgaacaac ttacaggaaa gaagttgatt ttagttggtg
     6901 atatagatat tgatgcaata ttagattatg caggaaataa aagaaataga aattctagta
     6961 aaaaaaatac atttaaatta aaaataccct ttagtacatt tatacaaatg cctagaaaga
     7021 tagaaaataa agataaaatt aatttaaagt atcttataca agatattact tcatctattt
     7081 tagatgataa tttatttatt agtgttactg caattataag ttatgaaaat agttccaaga
     7141 tagagtaatc tttaaattat agaaaggtgg atgcaatgag aataacgaaa actactttta
     7201 atgatgtatt ttcaagtgat gcaaatgaaa gtattataaa agtagaaaaa tatgttgata
     7261 aaaaatgtgc atctgtattt gagattttga cctacactat tattgttaca aatacaaata
     7321 ggtacaaaac tggaaatata tttttcaaag attatatttc taaatatata gagtttataa
     7381 ataatactgt aaaagttaat ggcattataa aacgtggatt agacccacag caaggttttt
     7441 acatagggag aatagatgca agttgtaaaa aaataatatc ttttaagagt gtagttttac
     7501 caaattctgc acataggatt ataaagaata gtgctaatat atactattat tataagtgta
     7561 atctagataa attcccaaca agaatatcaa tagaaagtaa tagagtttat actaatgtaa
     7621 ataaaattgt attcaaacaa ttgaatataa gtaatacttt aaaagctcct aaaaatctaa
     7681 gagatatatt aaaagtaaat gtaaatagta aaatatttga tgttaaacct atgaaaaatc
     7741 ttataaacaa aggtagtaat ttgaaattat gtaacttgat agtttttggg tgtatagaag
     7801 ttgaaataga ctatagttgt aaaaatagga gtaattttaa aacagtaaat aagtctaatg
     7861 acattaaaaa agataagtat tataaaaatg atatacaaaa taacaatgaa aatataaacg
     7921 attgtaaaaa gcaaataagt tttggtgata ataaggtaaa aaaaataaca aaaacttttg
     7981 gattttcttg tttcatttgt tctccagttg gaattattta tgaagatatg aaaaatataa
     8041 atataaaaat tgaacataca tcaataaatg aattaaatcc agaagaatta tttgtaaata
     8101 catcactttt attgtattat taagtatttt aaagctaaaa cttaaactta ttaaaatagc
     8161 atttggttaa aggaaaatag atattgatat atttatc
//
LOCUS       DAMJVI010000134         7943 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.139, whole genome shotgun sequence.
ACCESSION   DAMJVI010000134 DAMJVI010000000
VERSION     DAMJVI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.139"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            27..101
                     /locus_tag="PC545_003275"
     tRNA            27..101
                     /locus_tag="PC545_003275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC545_003276"
     tRNA            116..200
                     /locus_tag="PC545_003276"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC545_003277"
     tRNA            210..293
                     /locus_tag="PC545_003277"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            317..391
                     /locus_tag="PC545_003278"
     tRNA            317..391
                     /locus_tag="PC545_003278"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Gly,seq:gcc)
     gene            416..492
                     /locus_tag="PC545_003279"
     tRNA            416..492
                     /locus_tag="PC545_003279"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Arg,seq:tct)
     gene            502..577
                     /locus_tag="PC545_003280"
     tRNA            502..577
                     /locus_tag="PC545_003280"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Gln,seq:ttg)
     gene            586..661
                     /locus_tag="PC545_003281"
     tRNA            586..661
                     /locus_tag="PC545_003281"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:619..621,aa:Lys,seq:ttt)
     gene            664..752
                     /locus_tag="PC545_003282"
     tRNA            664..752
                     /locus_tag="PC545_003282"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Ser,seq:tga)
     gene            756..831
                     /locus_tag="PC545_003283"
     tRNA            756..831
                     /locus_tag="PC545_003283"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Phe,seq:gaa)
     gene            838..911
                     /locus_tag="PC545_003284"
     tRNA            838..911
                     /locus_tag="PC545_003284"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Met,seq:cat)
     gene            926..1002
                     /locus_tag="PC545_003285"
     tRNA            926..1002
                     /locus_tag="PC545_003285"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:960..962,aa:Ile,seq:cat)
     gene            1020..1096
                     /locus_tag="PC545_003286"
     tRNA            1020..1096
                     /locus_tag="PC545_003286"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:His,seq:gtg)
     gene            1105..1180
                     /locus_tag="PC545_003287"
     tRNA            1105..1180
                     /locus_tag="PC545_003287"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1138..1140,aa:Lys,seq:ttt)
     gene            1188..1261
                     /locus_tag="PC545_003288"
     tRNA            1188..1261
                     /locus_tag="PC545_003288"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Cys,seq:gca)
     gene            1269..1345
                     /locus_tag="PC545_003289"
     tRNA            1269..1345
                     /locus_tag="PC545_003289"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Arg,seq:acg)
     gene            1358..1433
                     /locus_tag="PC545_003290"
     tRNA            1358..1433
                     /locus_tag="PC545_003290"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1391..1393,aa:Val,seq:tac)
     gene            1509..1922
                     /locus_tag="PC545_003291"
     CDS             1509..1922
                     /locus_tag="PC545_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458013.1"
                     /translation="MYIVIGNEIVDSEELKIIIDKNSEFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLINEQCNLASLSDEEKFDEYMTLSDELALDLEELMPKYTIINARAYKL
                     DEVDGIVKIILAVAYADLGHLKLSDVVKRLSRQVD"
     gene            2231..5662
                     /locus_tag="PC545_003292"
     CDS             2231..5662
                     /locus_tag="PC545_003292"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDF2458014.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHREEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKSEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNVKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYNKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPAEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            5904..7832
                     /locus_tag="PC545_003293"
     CDS             5904..7832
                     /locus_tag="PC545_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDF2458015.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISSYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLRNSELVEIEKAKAGDIVVITKVNSLK
                     TGDTISADKDAEALEKIDFPKPQIYYAVTPKNKGDEEKVASVLNKLVEEDPTLHWYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGTADVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFTEEIFGGSVPKQYIPAVEKGLKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASAAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
BASE COUNT         3107 a         1015 c         1594 g         2227 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atataaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttat
      301 ttaatttaat aaacgagcgg gaatagttca gtggtagagc gcaaccttgc caaggttgaa
      361 gtcgcgagtt cgaatctcgt ttcccgctcc aaattttatg tttatgttgt aatgtgggtg
      421 catagctcag ctggatagag caacgccctt ctaaggcgtg tgtccggggt tcgaatccct
      481 gtgcgctcac cacatatata ttggggatta gccaagtcgg taaggcacat gactttgact
      541 catgtatgcg taggttcgag tcctgcatcc ccagccaaac agtatggtct attagctcag
      601 tcggtagagc acctgacttt taatcagggt gtcccgcgtt cgagtcgcgg atagatcacc
      661 aatggagagg tgtccgagtg gtttaaggag ctggtcttga aaaccagtga cttcgaaagg
      721 ggccgtgggt tcgaatccca ccctctccgc caaatgccca gatagctcag tcggtagagc
      781 aggggactga aaatccccgt gtcggtggtt cgattccgcc tctgggcacc aaaatgtggc
      841 ggtatagctt agttggctag agcgttcggt tcatacccga aaggtcatag gttcgactcc
      901 tattaccgct actaaaattt aatgtgggcc attagctcag ttggttagag cgcccggctc
      961 ataaccggta ggtctggggt tcgagtccct gatggcccac cacattaaat taacttatgg
     1021 tgggtatagc tcagttggtt agagcgccag attgtggctc tggaggtcgt gagttcgact
     1081 ctcattatcc accccaactt atatggtcta ttagctcagt cggtagagca cctgactttt
     1141 aatcagggtg tcccgcgttc gagtcgcgga tagatcacca aaaaggcggc gacatagcca
     1201 agtggtaagg cagtggactg caactccttg atccccagtt cgaatctggg tgtcgcctcc
     1261 aataaaatgc gctcatagct caactggata gagtgtctga ctacgaatca gaaggttagg
     1321 ggttcgagtc cctttgggcg caccatatga atagcgaggg attatagctc agctgggaga
     1381 gcacctgcct tacaagcagg gggtcacagg ttcgagccct gttaatccca ccagtgcaaa
     1441 cctctagtaa aactactaga ggttttattt ttgattgaaa aagtttttat atttaggagg
     1501 taaaaaattt gtatatagta ataggaaatg aaatagttga tagtgaagaa cttaaaataa
     1561 ttatagataa aaatagtgaa tttaaagttg aaaaagattt aagtaaaagc acaaaaagag
     1621 aagatgtaat agcatatcag ttaagtatag atttaaatta cctagattct cttataaatg
     1681 agcaatgtaa tttagccagt ctatctgatg aagagaaatt tgatgaatat atgactttgt
     1741 ctgatgaact tgcactggat ttagaagagt taatgccaaa atatacaata ataaatgcta
     1801 gagcatataa attagacgag gtagatggaa ttgtaaaaat aatactagct gtagcgtatg
     1861 cagatttagg gcacttaaaa ctttctgatg tagtaaagag gctatctaga caagtagatt
     1921 aggatatgaa tatgaactat attaaaaaaa tgtataaaat cttacgtaaa gatgaaaata
     1981 attatgtaaa catagaaaaa aacagtataa aatcaagtca attgtagaaa ttttcacata
     2041 aattcatctg aaattctgta aaaaatgcct taattttaat gaggtaaatc gagtttacac
     2101 agtctaaaaa ttacttatat ttataaattt atcttattaa ttaaggaact ttcttaaaca
     2161 tcttcaaaaa taaagtattt tagtctataa tctttaatat tacacaaaca ataaaggaga
     2221 agaatagagt atgcttaaaa agtttaataa aatactagtg gctaatagag gcgaaatagc
     2281 tataagaata tttagagcct gttcagaatt ggggataaaa agtgttggta tatacagtaa
     2341 ggaagataag tatggattgt ttagaacaaa agcggatgaa tcatatttga taggagaagg
     2401 taaagggcca atagatgcat atttagatat ggatggaata atagacctag caaagagaaa
     2461 aaaagttgat gctatacatc caggatatgg ttttctagcg gagaatgcag aatttgctcg
     2521 taaatgtgaa gagaatggaa ttacttttat aggaccatca tctaaggtaa tgaatatgat
     2581 gggggataag attaactcta aaaaaatcgc aaaagaagtt aatgttcaaa ctataccagg
     2641 agtagaaaaa gcaataagaa gtactgaaga agcaaaggaa gttgcaaaca aaataggata
     2701 tcctgttatg ataaaagcat ctaatggcgg tggcggtaga ggtatgagaa ttgtacatcg
     2761 cgaagaagac ttagagcttg aatatgaaac tgcttgtagt gaatcaagaa aagcttttgg
     2821 agaagatatt atatttatag aaaaatatat agctgatcca aaacatatag aagttcaaat
     2881 attaggtgat aattatggaa atatagttca cttatatgaa agagattgtt cagttcaaag
     2941 aagacatcaa aaaataatag aatatgcacc agctttttcg ttagatgata aagtaagaaa
     3001 agagatatgt gaagatgcag ttaagttatc aaaacatgtg ggttattcta atgcgggaac
     3061 attagaattt ttagtagatg caaatggtgg gcattatttt atagagatga acacaagagt
     3121 acaggttgaa catactgtaa cagagatggt tacaggaata gatatagttc aaagtcaaat
     3181 actaatagcc caaggttaca gcttagatag tgaagaaatt aatataaaat cacaagatga
     3241 tgtggaaata agaggttact ctatccaatg tagaataact acagaagacc ctaagaataa
     3301 attcatgcct gatacaggta aaattcaagt ttatagaact ggttcaggat ttggtataag
     3361 actagatgga ggaaatggat ttacaggggc aaacataagt cctcattatg atagtttatt
     3421 ggttaagaca atatcttggg atagaacatt ccaaggagca ataaataaaa ctataagatc
     3481 tataaaagaa cttagagtta gaggtgttaa aactaatgta ggattcctag ttaatgtttt
     3541 aaataatcct atattctcaa atggtaaatg tagtactaag tttatagatg aaaatccaga
     3601 tttatttgaa ataacagaaa gtaaagacag aggaacaaaa ttacttcaat ttataggaga
     3661 tgtaattgta aatgataatg cttgtaaaga aaaaccatta tttgatgcat tacatgaccc
     3721 aagaatggat aaggatggtt caaaatcaga aggaagtaaa atacttttcg ataagttagg
     3781 taaatctgca tatatagaaa agataaaaaa tgataagaaa ttactattaa cagatacaac
     3841 aatgagagat gcacatcaat cattattagc aacaagaata agaacatatg acttattaaa
     3901 agctgctaag ccaacagaaa aatatcaaaa ggatttattc tcacttgaaa tgtggggagg
     3961 agctacttat gatgttgcat atagattttt aaaagaatct ccttggcgta gattacaaaa
     4021 gctaagagaa gaaataccaa gcataatgtt ccaaatgttg cttagagcat caaatggagt
     4081 tggatataaa aactatccag ataatgtaat agaagaattt acaaaagaat ctgctagaca
     4141 aggtatagat gtatttagaa tatttgattc attaaactgg gttgaaaata tgaaaccatc
     4201 tattaataca gctttagaaa ctggaaaaat tgttgaagca actatgtgtt atacaggtga
     4261 tatactagat aagactaaaa ctaaatacaa tctagaatat tatattaaaa tggcacaaga
     4321 attagaaagc ttgggagcag atataatagc aataaaagat atgtcaggtc ttcttaaacc
     4381 atattcagct tacacactag ttaaagagtt aaagaaaaat gtaaaagcac caatacattt
     4441 acacactcat gatacaagtg gaaatggtgt agcaacttgt ttaatggcgt cagaagctgg
     4501 agtagacata attgatgctg cattagaatc tatggcagga cttacaagtc agccatcact
     4561 taatgctata gtagaggcac ttaagaatac agagagagat acaggaatag atttatttgg
     4621 atatgatgag ttaggaaaat attataagga tttaagaaaa gtttataata aatttgaaag
     4681 tgatttaact aattcttgtg cagaaatata taattttgaa ataccaggag gccaatatac
     4741 aaacttaaaa cctcaagctg atagtttagg attagttaat agatttgatg aagttaaaga
     4801 aaaatataaa gaagctaatg aagttgtagg agatataata aaagtaactc catcttcaaa
     4861 agttgtagga gacttagcta tctttatgac taaaaataaa ttagataaag ataatataat
     4921 agaagaaggt aaaaatttat cattcccaga ttctgttgtg gattactgta aaggtatgat
     4981 tggtcaacca gaaggtggaa taccaaaaga tttacaagag gtagttctaa aaggtgaaga
     5041 agctataaca gtaagaccag gttcattatt accagcagaa gattttgatg aaatagctaa
     5101 atatctaaat gaaaagtatg atataaatgc taatataaga aatgttataa gttatgcttt
     5161 atatccaaaa gtatatgaag attatatcaa acatctacaa cattataatg atatatctaa
     5221 acttgaaagt gatgtattct tctatggatt aaataaaaat gaagaatgtg aagtagaaat
     5281 agaagaaggt aaggtattaa caataagatt agttgaaatt ggtgaagtta aggaaaatgg
     5341 atttagaact ataggatttg agttaaatgg aatggttaga gaagtagaaa taaaagataa
     5401 gaatttctct ggaaaaatta ataatgttga aaaagctgat atgaatgacc ctcttcaaat
     5461 tggagcaagc ataccaggaa aagttataaa aataatggtt aaagaagaag atgaagtaaa
     5521 agcaaatcaa ccattaatcg ttatagaggc tatgaagatg gaaactataa tagttgctaa
     5581 gactgatgga gtaattaagt caatcaaagt aaaagaagat gatatggttg aagataaaca
     5641 attattaatg ataatgaagt aatttaatta aaataaatta aaaagcactc tgaaatgttt
     5701 aataaaaaat gttttagagt gtttttattt attttaatta aatatttgtt aaaaaaaaga
     5761 ggacaattac ctccagatgt agaaataaag ctagtaagga aaatgttatc taaaaattct
     5821 aaatatagta aatattttcc ataatatttc ttaggaaata acagcaaaga taaaatacat
     5881 aaatttaaaa ggtgggtgct tatatgaagg tttatgatag taaaatgttg agaaatgtag
     5941 cggtattagg acatagcgga tgtggaaaaa caaatctaat cgaaactata gcttacacag
     6001 caaacactaa taagatacct aaattaacag acaaggtaaa tatgacttat agcatgggat
     6061 taatacctat agagtataat gattataagt ttaacttatt ggatacccca ggatattttg
     6121 actttagcgg agacgttgtg tcatctctta gagcaagtga tgcagcaata atagtaatag
     6181 atgcaacagc gcctattcaa gttggtacag agaaatcatt agaactaact gagagtattc
     6241 ctaagataat gtttataaat aaaatagata atgaaaaggc aagatacaaa gatgcaatag
     6301 caatgttaag agaaaaatac aataataaga tagttcctat gataagtcct atatacaaag
     6361 ataaaaactt tgtaaagtta cataatgttt ttgaaaacat agatgattta gaaggtgaat
     6421 ttaaggaaca agctatgagt gttaaagaag cattaatgga acttatagca gaaactgatg
     6481 accaaatatt agataaatat tttaatggag aagaactaac tacagaagag atacaaaaag
     6541 gtattattat aggtatacaa agaggtgata ttatacctgt aatatgtggt tcaactataa
     6601 ataatatagg cacaaaggaa atattagata caatatcaag ttatttagaa cctatcttta
     6661 cagaggaaag caaaccattt aggggattag tatttaaaac tatggttgac ccatttgttg
     6721 gaaagatgtc atatataaaa attactgagg gtgtactttc taaggataaa gatgttttta
     6781 atataaataa aaatgtaaaa gaaaaaatag ctaatatata tacattgaga aatagtgaat
     6841 tagtagaaat agaaaaagct aaagctggtg atatagtagt tataactaaa gttaattctc
     6901 taaaaacagg agacactata tctgcagata aagatgcaga agctttagaa aagatagatt
     6961 ttcctaaacc acaaatatat tatgcagtaa ctcctaagaa taaaggtgat gaagaaaaag
     7021 tagcatctgt attaaataaa ctagtagaag aggaccctac attacattgg tatagaaata
     7081 cagagacaaa acaagcactt ttaggaggtc aaggagaact tcatataaaa acaattaaaa
     7141 ataaaatgaa agataagttt ggtgttgatg ttgaattgaa tgaccttaaa gttccttata
     7201 gagaaactat aaaaggaaca gctgatgtac aaggaaaaca taaaaagcaa tcaggtggtc
     7261 atggtcaata tggagacgta aaaataagat ttgaaagatg tgaatctgat tttgaattta
     7321 cagaagaaat atttggtggt tctgtaccaa aacaatatat accagcagtt gaaaaaggtt
     7381 taaaagactc tatgcaaaaa ggtatattag caggatatcc tgttacaaac ataaaagcta
     7441 cgctttatga tggttcatac catgatgtag actcttctga aatggccttt aagatggcag
     7501 caagtgcagc ctttaaaaaa ggtatggaag aagcacaccc aatactacta gaacctataa
     7561 tgaaacttaa gataactgtt ccagaagaat atatgggaga tgtcatgggt gatataaata
     7621 agagaagagg aaaaatattt ggaatggaac cagatgacaa agggaaacaa ataatatttg
     7681 cagaagctcc acaagcagag acatttaaat atgcaataga tttaagagca atgacacaag
     7741 gtagaggata ctttgaaatg gaattagaga gatatggaga ggttccatca cagtttgcag
     7801 aaaaaataat tgggttagct actgcaaagt aatgaacaaa ataaatagaa aaataattaa
     7861 tatatattat taaaaatatt tttagggttt taactaggta taggtttatg ttaggtaaac
     7921 ttatacctag tttttataca ttt
//
LOCUS       DAMJVI010000135         7933 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.140, whole genome shotgun sequence.
ACCESSION   DAMJVI010000135 DAMJVI010000000
VERSION     DAMJVI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.140"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(26..739)
                     /locus_tag="PC545_003294"
     CDS             complement(26..739)
                     /locus_tag="PC545_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HDF2458016.1"
                     /translation="MGKVAKILLSLIVIVLVLVGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLVNSQYELELRPTLKDGNLVVSLENVKIGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTSVG"
     gene            1031..2695
                     /locus_tag="PC545_003295"
     CDS             1031..2695
                     /locus_tag="PC545_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HDF2458017.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPEDKIEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDIPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPGK"
     gene            complement(2802..3701)
                     /locus_tag="PC545_003296"
     CDS             complement(2802..3701)
                     /locus_tag="PC545_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDF2458018.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEKNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELDIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVHIECEDSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3728..4366)
                     /locus_tag="PC545_003297"
     CDS             complement(3728..4366)
                     /locus_tag="PC545_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2458019.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KAGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKTTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4703..5701)
                     /locus_tag="PC545_003298"
     CDS             complement(4703..5701)
                     /locus_tag="PC545_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HDF2458020.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KKISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYDYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KAFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADLIRNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5799..6374)
                     /locus_tag="PC545_003299"
     CDS             complement(5799..6374)
                     /locus_tag="PC545_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458021.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6690..6953
                     /locus_tag="PC545_003300"
     CDS             6690..6953
                     /locus_tag="PC545_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458022.1"
                     /translation="MIKNTKQITVEVNEILLQEMSKITNYENNILTDLINESIVNYIL
                     ENKKHNIENKDLEEVIKFYKKENTEKPKSAARAIAEAKTHTLY"
     gene            complement(7390..7767)
                     /locus_tag="PC545_003301"
     CDS             complement(7390..7767)
                     /locus_tag="PC545_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2458023.1"
                     /translation="MTITKIPPAELKVMKFIWSSDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
BASE COUNT         2791 a         1217 c          836 g         3089 t
ORIGIN      
        1 atataaatat aagttttcta tatttttaac caacagatgt tttcttcaaa ttaatcagat
       61 gatttagtat atcttgaacc tttccagagt cctttaaata tcctaatgcc ccatttattt
      121 gtgattttaa atctatttct acttccatat ccaaatcaac atttcctttt tttatagata
      181 tatttttaag tgttattgga taaagataac ttttttccaa agtaatctta tttccattaa
      241 cctcaaatgg aactttttta ttgtagttac ttaatatttt ttctagcatt ttatcactta
      301 gtttaaattt tccaattttc acattctcaa gacttacaac taagtttcca tcctttaaag
      361 taggtctcaa ttcaagttca tattgactat ttaccaaacc aaatacctta tatggtcctg
      421 ctacctttat tttaccattt ttcatttcaa caaaattatt ttcaagttca ttaattccat
      481 gcttctgcat caatgtatat attaaatttt taaactcttc ctctgaaaca gatatttttc
      541 caaccattct aagtggattt tttactacct ccatagaatt tatatatgag ccttttgata
      601 aatattcttc atctatgatt ttattatttt gtgaactaac atcatatctt tcttttggtg
      661 tcaagatata tacaacaatt cctacaagta ctaaaacaat aacaattaaa cttaacaaaa
      721 tctttgcaac ctttcccata atcgtctcct ttacaaatct gtcatcagat ttaaaataaa
      781 tttagataac taacttcaca taatattctt aacaatatac atactataat acagtctttt
      841 aaaaattaat tcaaccaatc taatgttttg taaataaact gtaaaaacat gtttttagta
      901 taaaagttaa tattttgcat aatactctta attacacctt tatttttttc taatcttgga
      961 ttaaaattaa tattatgcaa tacagtgtag tgttctgtat tataatcaaa ttaaatagga
     1021 ggtatagaat atgtcaaatg aaacgaactc atctaacttt ataaagaata ttattataaa
     1081 cgacttagaa acaggaaaac atgacagtat cataactcgt tttccacctg agccaaacgg
     1141 atatttacat attggtcatg caaaaagtat atgtcttaat tttggattag ctaaggagtt
     1201 taatggaaaa gctaatttaa gatttgatga tacaaaccca ttaaaagagg atgttgaata
     1261 tgtagagtcc ataaaagaag atgtaaagtg gttaggattt gattggaacg agttaaattt
     1321 tgcatctaac tactttgatg aaatgtataa gagagcttta atactaataa aaaagggtaa
     1381 agcttatgta tgtgatttga cacaagagga aatgagagaa tatcgtggta ctctaactga
     1441 accaggaaaa gaaagtcctc atagaaacag aactattgaa gaaaaccttg atttatttga
     1501 aagaatgaaa aatggtgaat ttaaagatgg tgaaaaaaca ttaagagcca aaatagatat
     1561 gtcttctcca aatataaacc ttagagaccc aattatatat agaatatctc attcaacaca
     1621 ccataataca ggagataaat ggtgcatata tcctatgtat gcttttgctc atccaattga
     1681 agatgcaatt gaaggaataa ctcactctat atgtacttta gagtttgaag accaaagacc
     1741 tctttatgat tggtttgtta aagaatgtga gatggaaaat atacctagac aaatagaatt
     1801 tgctagactt aatataaata atactgtcat gagtaaaagg aaattaaaac aacttgtaga
     1861 tgagggtatt gttgatggtt gggatgaccc tcgtgttcct acaatatccg gaatcagacg
     1921 taagggatat actgcagaag cattgcgcaa tttctgtagt gaaatagggg tatcaaaagt
     1981 taactctacg gtagatagtc aaatgcttga ttatttccta agagaaaact tacaaccaaa
     2041 ggctcctctt actatgggag ttttaagacc tttaaaactt attataacta attacccaga
     2101 agataagatt gagatgcttg aaatagagaa taatgctaaa gatgaatctc aaggaaagag
     2161 attagttcct ttctctagag aattgtatat agaacaagat gattttatgg aagaacctgt
     2221 taagaaatat tttagatttt tcccaggaaa tgaagtccgt ttaaaaggtg cttattttgt
     2281 aaaatgtact gatgtaatta aggatgaaaa tggaaatgta gttgaaatac atggtactta
     2341 tgaccctgaa actaagagtg gctctggctt tacaggacgt aaggtaaaat ctacaataca
     2401 ttgggttgat gctaaatcag ctatcccatg tgaatttaga ttatttgaac cattaattct
     2461 tgatgatata cctgaaaatg aaggtaaaca tttcttagaa caaataaatc ctaattcatt
     2521 ggaaatatta caaggttttg ttgaacctac acagataaaa gatgctaaac cttttgataa
     2581 attccaattt gttagaaatg gattctttag tatagataat aaatatacaa ctgatgaaaa
     2641 atttgtattt aatagaatag ttcctctaaa aagttctttt aaaccaggta aataatctta
     2701 caatcaaaaa atgaagactt aaaatataaa ttacttctac tataaataaa cttagttaga
     2761 agtaattttt tatttgagtc ttcatcattt actttaaatg attaactttt ttcactacag
     2821 attcccatga cttctattcc atcttttatg cattctatgt taagaggaaa atctggtcct
     2881 ttttctccgt caatatctgt tataagtgaa tcttcacatt ctatatgcac ctttttagtt
     2941 ttaaagtata gtatttcatt tccattatat tgatctaaat aatcacctct aagcacactt
     3001 atcaaaactg gtattgattt tggtattggc attcctttaa aaataattac atccaaatat
     3061 ccatcatcta tttcagcctt atatgctaga tttatatttc ctgctgtttt gccattaaaa
     3121 atcaacataa gatacatatc tccatcataa tacatttcct cagaagatac ttttatctta
     3181 aatttcctca tatacaatgc ttcttctatt ccctttatat aataagaaat tcttccaaat
     3241 gaatttttta agtctgtgtt tatcctttgt gatacatccg taaacatacc agcacttgca
     3301 acatttataa agtacttatt attgactttc cctatgtcta ttttctttgg ttttgaattt
     3361 atgatttcat ttatagcttc ttttatatca aatgaaattt gaagagcatt tgaaaaatca
     3421 tttgcagttc ctgtaggcaa aattccaatt ggaattttta tgtctaattc tttcattgca
     3481 tttactacta aatctacagt accatcacca ccagctatca atatgtgttc ataagaacta
     3541 tcaacttcct taaatgcatt ctttacaggt tctttaatag ttaatctata gggtatcaat
     3601 aaataatttt tttcttgata taattctatg atagtgtcta aatttgataa tatctttttt
     3661 tcacctgaat taggattgta tataagtttt acttttttca ttatttctcc ttactcattt
     3721 tattctttta gtatccaaaa tcttcattta aaaaaatcac atgtattctt tctggtgtta
     3781 attgtctttt tataagtgta taactacagc atattcttcc tggacttgaa cagtctatac
     3841 atataccagt agtcttacat ccagtatttg tacttaatct tttacaattg gctggagcag
     3901 ctattgtttt tgttcttttt atagcttcat ctacatcttt aactatttta tttactcctg
     3961 ctataactat tactttatca ggtccatata tcatagcagc aactctattc ccattcccat
     4021 ctaaattgta aagttctcca tcctctgtta tcgcattagt acttgtaaaa taagcatctg
     4081 cagaaaatga ctttctatat atttctttca tttcttctgg tttgatatct tttgcatatc
     4141 tatcaagaaa attatatttt tctcttataa aatcaatcac tcctgtttca aataaggtca
     4201 tagaacctcc acatcctact agagaacctg ctttaactat ttcttcaatt tttttaagaa
     4261 gctcttcttt gttacttact aaataccctt tcatattatt gttttccaaa gattttattg
     4321 ttttttctat tcttttttca ttaagccaat ttaaattttg atccatttta acttccccct
     4381 tatttttaat atgtacttaa ttatttataa actttatttt ctttataagt ttattttagt
     4441 acatctaaaa aaaaatacaa tataaaactt aaatagtttt acataatttt ttccaaaata
     4501 acttactcaa tcaaaaaagg aaagtagatg tactattcta ctttccctat attgtatgta
     4561 gtatttattg cctaaaatct atatattaat tatatgtttc atattatata atatcacagt
     4621 taattaaaat gtaatactat cttcttttta aacatataaa ttgtattatt aaatttaata
     4681 tatccaactt caataaatct tattaatttg atgaagaaga tgctatagct gcacttgttg
     4741 ctgccattac acatgcaatt tgaatagcaa caacaatatt taaaaatata ttattagtta
     4801 tagagcctat attattctca tttcgtatca aatctgcata aattgaagct acaatagcag
     4861 aactaatcat tactctatta ctttttccta aagaccaatt tccaaagcct ttttcttctt
     4921 taagtttatt tgaaacattt tttatctctt ctattatttc atcttctcta taatctaata
     4981 atgaaattgc tcctattatt ggatatccat aataattcat tttacaatca ttttctttaa
     5041 atgctttttt aagttcaata gtctttttac atttaagcat agttctatca tcactaaata
     5101 aaagaatttg ggataatgct tgtaaatcat ttcctttata aaaaccattt tcatttagaa
     5161 attcataaca tttttctatg tattctaaat cttcatctaa attttttgaa tgaattgcaa
     5221 taagtgctat attacaatag tcatcactag aagttagaaa tggatgtttt ttcctcatgt
     5281 aatcatatat tattttcatt tttgatatac atgtaagata atccattttc tctctatttt
     5341 cataaactat tgatgctgta agtggtaaat atgtatcgcc ccaaaacttt tcacttttaa
     5401 gttctttgta tatatctaaa atcatcttaa aactactttc tggatttgat tcaaacgaaa
     5461 gtagtgttgc catataaaac atattatatc ctctaaaatt agagaataat ccagtatttt
     5521 ttttaataag ctctattcgt tcttccacta atctaggact tattttcttg ttcttaaggg
     5581 taaatgcaag tgcacaacta tgttgtatca atcccatttg ccaagaacct ttaacttcat
     5641 ttaatgcttt gtaattttct attaataagt ttacttttcc atttataaac gattgttcca
     5701 tattgccctc ctagtaatat atctcattgt actaatagtt ttatatttat tatatatata
     5761 ttaaatacta caatcaatac atattacttc aaagaacatc attcttcaac aataatgtag
     5821 ccttttttgt aatgtcttcc catattattg actctatcat agtactctga ttcactttca
     5881 ttactccatt tgggcacaaa tacatctatt gcattagaat tactaaaata cttactagac
     5941 cccctatcca taacagtttt taattttcca tctacaataa tcttagttcc aaaacttaag
     6001 tgattgtttg ctacaaaacc atcttctaaa tcatctcctg tagcagttat cgctccatat
     6061 ccattttctt gattaagact tgtgtaataa ctaacttcaa aagttatagg tactaacttc
     6121 tttattttaa tacctaactg attttgtagt ttctcctttt gtactttcaa cttatactca
     6181 tcactatttt tataatcttc cataccttga ttatacttgg aattaaagct tttacctgac
     6241 tttactatag tatctatatt tactatagat tctttacaat gatctgcatc tatttttgtt
     6301 tctccattta ccaataatgg agttaaacat acactacata caacacatgt aatcaatgct
     6361 tctttaagct tcataaattc cccccttatt attttgatat attagcatat ttaatattat
     6421 atgtctacat ttagatttaa ataccacacc catttgcttt aagaagttgt cttatattta
     6481 attcacctta aacacttata aatcctctat cttagaatca tatagaataa agcttttatc
     6541 attaaatatt ttttattaat cttttaaaat ctaaaactac tttatctaat tttctctttt
     6601 ttagaattta agaattaaaa ttctataatt ttaaaacata ttgaatttat ttttttgaga
     6661 tgatataatt ttcttaagag gtggttgata tgataaaaaa tacaaaacaa attacagtag
     6721 aagttaatga aattttatta caagaaatgt ctaagataac taattacgaa aataatattt
     6781 tgacagattt gataaatgaa tcaattgtaa attatattct tgaaaacaaa aaacataata
     6841 ttgaaaataa agatttagaa gaagtgatta aattttataa aaaagaaaat actgaaaaac
     6901 ctaaaagtgc agcaagagct atagctgaag caaaaactca tactctgtat taatcaaata
     6961 aaaaagttcc taaaaactag gaactttttt atattaataa ttattaatat atctcaatat
     7021 ttatctcaaa tctatcaata taagcatgtt taatacaatc tattgtatac ttatcttacc
     7081 ctagaaataa tcaaaacaaa aataaaatta tgccctttat atctaaaaca attaatttac
     7141 atgattaata tacaaaaatt ctgactttta aattcgtata caatatcaaa ctaaaataat
     7201 attaatgaca aacccaatat aaattaaatt caaacaggtt tgattttaca tcaaacttat
     7261 tgtattaata ttaaataaca agacaaaaat atttataaaa atcacaaatt ttacataaaa
     7321 ctttatattt gattcattta actaattatt gtaaaaacta tttattttat gataattaac
     7381 tacaccaaat tattcatctt ttttaatatc tttaacccat ttttcaacag agttcaattc
     7441 ttcttcattt accccctttt tgaacaagga tttcataaaa ctctcaatag aacttccatg
     7501 catattatct aaaaaagttt ttgtttcaaa atgtaaatac tcttcttctc caataagaac
     7561 tgtataatgt gtatgtctat caatttttct tgcacttaag aattgcttat ttactaatct
     7621 tgataaaagt gttagtgttg tagtttgctt ccaattatat agtttttcca tagcttcaat
     7681 gacatctttt gatgctactg taaaatctga actccaaata aatttcatga cttttaattc
     7741 tgctggtggt atcttagtaa tagtcatatg attttacact cctctcaaac ttctacataa
     7801 ttaaatacta tattaataga cttttcgagt caaaggtttt atttaaaata tctcatccaa
     7861 atttattaat acaaaaaatt tatattttta ttcaaaatta aacacacaat ctattaatat
     7921 aaagataaat atc
//
LOCUS       DAMJVI010000136         7883 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.141, whole genome shotgun sequence.
ACCESSION   DAMJVI010000136 DAMJVI010000000
VERSION     DAMJVI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.141"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            81..1532
                     /locus_tag="PC545_003302"
     CDS             81..1532
                     /locus_tag="PC545_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDF2458024.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFNNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLEKVSEWHRELSEEHLG"
     gene            complement(1730..2371)
                     /locus_tag="PC545_003303"
     CDS             complement(1730..2371)
                     /locus_tag="PC545_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDF2458025.1"
                     /translation="MTDNYIFDSYNNLVDGLSAYLGEGYEFILYKFNGVHYSINKIAN
                     GKYNTVKEGDIITDFDLALINKLENDADGDFISYFNRNKLGEPLKSTDIVIRGVDNKI
                     IGLLCIHFYLNTPLTRIIGSYIPEHNAMQSAPVLENIVEDTDEVILKSYQDAKVIVEN
                     DSNISSNDKNKSIVSILNESGIFKIKDSVVKVADLMGLSKNTIYMHLRNLNKN"
     gene            2845..4224
                     /locus_tag="PC545_003304"
     CDS             2845..4224
                     /locus_tag="PC545_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2458026.1"
                     /translation="MAQDKIVVEILENIGGKENIKEVAHCMTRLRIKVCDSSQVNQEK
                     LKKIKGVLGLVLKGEQYQIVIGPNVSQVYNTLNEVIGIIPEKDSKKTISEGKQNSIAK
                     ILDTIAGIFNPIVPALAGAGLIKAILALLVASKFLSADSETYIILNTISDGVFSFLPF
                     MLAYSAAKIFKMNTYVAVAIAGAMLHPNFINLIQQGLTKISFIGIPMSLIQYNGSVFP
                     IVLTIWFASYIEKGVDKITPKALKIILVPALTILFAAPIALIIIGPIAQMLGNYIAGG
                     ASILFERGGIFAGLIYGGIYSFMVVTGLHHGMVPVLVDGITRYGFNYISPVSGSSNMA
                     QAGSAFGVWLKAKDKDLKAVAATATISALTGVTEPAIYGVNLRLKKPFLCAAIGGAAG
                     GGFAAYFQCKAFAMGGPSFLTLPMFIGEGGHVGLVAIAFTIAFIVSAIATYIVGFEEE
                     VTADSHANL"
     gene            4268..5002
                     /locus_tag="PC545_003305"
     CDS             4268..5002
                     /locus_tag="PC545_003305"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010990049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDF2458027.1"
                     /translation="MKIYLTKDYEEMSQVAAQMVLKYMYSNDNKRINLSITAGQTPKR
                     TYEILSEFVKNKPYFENVHYYNFDEIPIKGEKVGVTIRNLTNMYFREAQIPEERIHVL
                     DENNYQGFDRKIEADGGIDMVLMGLGWDGHFCGNLSGTVNSFDEETRAVSTYITDDMA
                     KLLTKSVGGNPEKLFDYYVTYGPKTIMHCKNIIMIVNGKHKAQILKQTLFGPLDLLCP
                     SSLLRLHPNFTLIVDEEAASEIKDLI"
     gene            5091..6290
                     /locus_tag="PC545_003306"
     CDS             5091..6290
                     /locus_tag="PC545_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2458028.1"
                     /translation="MYNHMKKVFQGLEGGLFSSVEKADVGDSYQKLGEQGVSLMGWAD
                     PFFPDYCIPRHILDAATLAISSNMSTHYTAPVGNDLLKIEIAEKLKEVNNLDVDFKRN
                     ILITPGSDSGLYYSMLPFINEGDEVLIPTPCYPNNIQNTKLMGGVPIFVELKESDGYQ
                     IDITEFEKRLTNKTKLVVLTHPNNPTTTIFNKESLEKLAKFIIDNDLILICDQAFEDF
                     TFENELITPASLEGMFERTVTIFSTSKGMGLSGFRVAYMVCSDKLMDKYYGAAVTVLG
                     ATNTVAQMSTIEAFKDRSFMEDFERVFDKRRQEAYKIINEIPNVSMQMPESGFLGWIN
                     VSKLGTSSEIQKYLVDEAKVCINDGINYGPGGEGHLRIILGSYKDDSKVIGALNRIKD
                     ALIKKQQ"
     gene            6314..6796
                     /locus_tag="PC545_003307"
     CDS             6314..6796
                     /locus_tag="PC545_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2458029.1"
                     /translation="MLSIFKSKKNDNKLKAFASGKVIPIEDVDDNVFSSKMLGDGIAI
                     YPTNEYVVAPCDAEVNIIMKDTKHAVGIKLFNGMEILFHVGIDTVSMNGEGFEIFVSP
                     GDKIKRGDKLISFNSKLIKEEGLQDVVIMVVTNSEQYKNIKYHIGLEAVIDDTIIAEI
                     "
     gene            complement(7114..7711)
                     /locus_tag="PC545_003308"
                     /pseudo
     CDS             complement(7114..7711)
                     /locus_tag="PC545_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2909 a          907 c         1347 g         2720 t
ORIGIN      
        1 tttttaacaa ggaattattc atttttttga ttttaagatt tattgagaaa acaaaactaa
       61 ataggggggt aattaatata atggattttt tcaatggtat tttaacgagt attaacaatg
      121 cagtagtatt tgtaaacaat tacttatggt cgtatatctt aattataatg ttagttgtaa
      181 ttggtattta ctttactatt aaaacaaatt ttgtacaatt cagatatttt atagaaatgt
      241 ttagacttct gggtgatggt acagcgaata aaaatgcaaa aaaggaagga aaaatctctt
      301 catttcaagc attttgtata agtacatctt caagagttgg aactggaaat atagctggaa
      361 tagcaattgc agtggtagca ggaggacctg gtgctgtatt ttggatgtgg cttatagcat
      421 taattggttc tgcatcaagc tttgttgaga gtacattagc tcagatttac aaagtgaaag
      481 atggtcaagc ttttagaggt ggaccagcgt attatatgga acaaggtcta aataaaaaat
      541 ggatgggtat tttattctca atacttataa ctatatgtta tggttttgta tttaatgcag
      601 ttcaagcaaa tactgtttca cttgcattta ataatgcatt tggaataagt aaaatggcta
      661 tgggaatagg tcttggaatt ttaactgcaa tagttatttt tggtggagtg catagagttg
      721 caaaagtttc tgaaataata gttcctgtat ttgcaggatt atatatatta gttgctttgg
      781 ctatagttgt aatgaatata acagagatac caagtgttat agcattgata gttgaaagtg
      841 catttgattt taaaggaatg gcgattggta catttatggg agttgtaatg actggagtga
      901 agagaggtct attttctaat gaagcaggta tgggtagtgc tcctaatgct gctgcaacag
      961 cacatgtaac tcatcctgta aaacaaggtt taattcaatc tttaggtgtt tttacagata
     1021 ctataataat atgtagttgt acagcattca tggtattatt atattcaggt tatagtagta
     1081 gtggagcaac tggaattgaa ttagctcaag aagctttaac tcatcatata ggaccagttg
     1141 gaaatatatt tatagcagtt tgtatattct tgtttgcatt tagttctata gttggtaatt
     1201 actattatgg tgaatcaaat atggagttta tgagtggaag taagactaaa cttaatgtat
     1261 ttagagtttt tgttgtaggt atggttttat ttggttcact tactcaagtg gatgtagttt
     1321 ggaatcttgc agacttattt atggcattga tggcgataat aaacttaata gcaatagcac
     1381 ttttaggaaa atatgcattt attgctttaa aagattatac aagtcaaaaa aaatcaggaa
     1441 taaaagaccc tgtgtttatt gcagatgaaa tagaaggcct agaaaaggta tcagagtggc
     1501 atagggaatt atctgaggaa catttaggat aaaaaatagt ataaaattat aaaaccattt
     1561 tgaaagacta gagtatgtat atactttata tgaatataat attgaattaa tttatattac
     1621 tggaattttt aattccagta atattttttt aattacaagg atattatgat gacttaaaga
     1681 ctgtggaaaa ctctttttta gttttcatta ttaatattaa ttgacaaatt tagtttttat
     1741 ttaaatttct taagtgcata taaatcgtat tttttgataa tcccattaag tctgcaactt
     1801 taacaacaga atccttaatt ttaaaaatac cactttcatt taaaatagaa acaatagatt
     1861 tatttttatc attgctagat atattagaat cattttcaac aattaccttt gcatcttggt
     1921 atgattttaa aattacttca tcagtatctt ctacgatatt ttctaaaaca ggagcacttt
     1981 gcatagcatt atgttccgga atatatgaac caattattct tgttagtggt gtattaagat
     2041 aaaaatgtat gcagagtaga ccgattattt tattatcaac tccccttatc actatatctg
     2101 ttgattttaa aggttcacct agtttgtttc tattaaaata actgataaaa tctccatcag
     2161 catcattttc gagtttatta atgagtgcca aatcaaaatc agttataata tctccttctt
     2221 ttacagtatt atatttacca ttagctattt tatttattga atagtgtact ccattaaatt
     2281 tatataaaat aaactcgtat ccttctccta aatacgcaga taatccatcc actagattgt
     2341 tataagaatc gaaaatatag ttatcagtca tgttaaaagt catatgttac ctcctaagaa
     2401 atatcttaat atcatattaa catataaaat gaatttaata aaatatattt cagtgaaata
     2461 ataaatataa tgaaaaaaaa actattgact gaaaaatatt tcagtgatat tattaaaata
     2521 cagtaaggca aacgtattat tgacagaata tataaaatgt taaaaatagt atctaggaat
     2581 taaacaaatt aaaggactag tgatttgaat ttttttagta taatatgtga cttccaatta
     2641 atatttatta aacaccaata aaattgtaaa tatgcttaat gatatacttt agcgatgaag
     2701 tgaaaatatt ttatttttat gattaaccta taggaattaa tttagcatac tgtttagcta
     2761 gtaaatctaa ataaatttta gctttagctg atatcatgtt gaataaagtt tctattggat
     2821 tttgtataac aaaggagtgg ggagatggca caagataaaa ttgtggttga aattttagaa
     2881 aacattggtg gtaaggaaaa tattaaggaa gtagcacatt gtatgactag actgagaata
     2941 aaggtatgtg atagttctca ggtaaatcag gaaaaactaa agaaaataaa aggtgtacta
     3001 ggtcttgtat taaaaggtga gcaatatcaa attgttatag gtcctaatgt atcacaagtg
     3061 tataatactc ttaatgaagt tataggaatt atacctgaga aggatagtaa aaaaacaata
     3121 tctgaaggta aacaaaatag tattgcaaaa atattagata ctattgcagg tatttttaat
     3181 cctatagttc cagcattagc gggtgcaggt ctaataaaag ctatattagc attactagta
     3241 gcatcaaagt ttttatctgc agatagtgaa acctatataa tattaaatac aatatctgat
     3301 ggtgtatttt catttttacc atttatgtta gcttactcag cagctaaaat atttaaaatg
     3361 aatacttatg ttgctgtagc aatagcagga gcaatgctac atcctaattt cattaattta
     3421 attcaacaag gcttaactaa aatatctttt atcggtattc cgatgtcact aatccagtat
     3481 aacggaagtg tatttccgat agtactaact atttggtttg catcgtatat agaaaaagga
     3541 gtagataaaa taacaccaaa ggcacttaag ataatattag tacctgcatt gactatttta
     3601 tttgcagctc ctatagcatt aattattatt ggaccaatag ctcaaatgct aggaaactat
     3661 atagccggtg gagcaagtat cctatttgaa agaggaggaa tatttgcagg gttaatttac
     3721 ggaggaattt attcattcat ggtagtaaca ggattacatc atggtatggt tcctgtatta
     3781 gtagatggaa taacaagata tggatttaac tatataagtc ctgtgtcagg tagttcaaat
     3841 atggctcaag caggatctgc atttggagta tggttaaaag caaaagataa agatttgaag
     3901 gctgtagcag ctacagctac catatcagca ttaactggag ttaccgaacc tgcaatatat
     3961 ggtgttaatt taagattaaa gaaaccgttt ttatgcgctg ctataggtgg tgcagcagga
     4021 ggtggatttg ctgcatattt ccaatgtaaa gcatttgcta tgggaggtcc atcgttttta
     4081 actcttccta tgtttatagg agaaggtgga catgtaggat tggtagcaat agcatttaca
     4141 atagcattta tagtatctgc aatagctacc tatatagtag gatttgaaga agaggtaaca
     4201 gcagattcac acgctaactt ataataaaga gaaattattt aacatatcaa aaaggagatt
     4261 aattactatg aaaatttatt taactaaaga ttatgaagaa atgagccaag tggcagcaca
     4321 aatggtgtta aagtatatgt acagcaatga taataaaaga ataaaccttt ctataacagc
     4381 aggtcaaaca ccaaaaagaa catacgaaat attaagtgaa tttgttaaga ataaacctta
     4441 ttttgaaaat gtacattatt ataactttga tgaaatacca ataaaaggcg aaaaagttgg
     4501 agttactata agaaatttaa ctaatatgta ctttagagaa gctcaaatac cagaagaaag
     4561 aatacatgtg ttagatgaaa ataattatca aggctttgat agaaagatag aagccgatgg
     4621 tggaatagac atggtattaa tgggattagg ttgggatgga cacttctgtg gaaatcttag
     4681 tggtacagtc aactcttttg atgaagagac aagagctgtt tcaacttata taacagatga
     4741 tatggctaaa ctattaacaa aatctgtagg tggaaatcca gaaaaattat ttgattacta
     4801 tgtgacatat ggacctaaaa caataatgca ttgtaaaaat ataataatga tagtaaacgg
     4861 aaaacacaaa gctcaaatat taaagcaaac actatttgga ccattagatt tattatgtcc
     4921 atcatcttta ttgagattac atcctaactt cacattaata gtagatgaag aagcagcatc
     4981 tgaaataaaa gatttaatat aaataagtaa ggaattaaaa ttaaaaatga atttaggtgc
     5041 aaagtaatat tttattttgc acctttgctt taataaaaag gagaataaaa atgtataatc
     5101 atatgaaaaa agtattccaa ggattagagg gtgggttatt ttcaagtgta gagaaagctg
     5161 atgtaggcga ttcttatcaa aaattaggtg agcagggtgt aagtttaatg ggatgggcag
     5221 atccattttt cccagattac tgtataccta gacatatatt agatgcagct acattagcga
     5281 tatcaagtaa tatgtcaact cattatacag cgcctgtagg taatgactta ttaaaaattg
     5341 agatagcaga aaaattaaaa gaagtaaata atttagatgt tgattttaaa agaaatatat
     5401 taataactcc tgggtcagac tcagggcttt attactcaat gttaccgttt ataaatgaag
     5461 gggatgaggt tcttatacca acaccatgct atccaaataa tatacaaaat actaaattaa
     5521 tgggaggagt tcctatattt gtagagttaa aggaatcaga tggatatcaa atagatataa
     5581 cagaatttga gaagagatta actaataaga cgaagttagt agttcttact catcctaata
     5641 atccaacaac tacaatattt aataaagaat ctttagaaaa attagctaaa tttattatag
     5701 acaatgattt aatattaata tgtgatcaag catttgaaga ttttactttt gaaaatgaat
     5761 taataacacc agcttcatta gaaggtatgt ttgaaagaac agtaacaata ttttcaacat
     5821 caaaaggtat gggattaagt ggatttagag ttgcatatat ggtatgcagc gataaattaa
     5881 tggacaaata ctatggagca gcagtgacag tattaggtgc tactaataca gtagctcaaa
     5941 tgtcaacgat tgaagcattt aaagatagaa gctttatgga ggattttgaa agagtttttg
     6001 ataaaagaag gcaagaagca tataagataa tcaatgaaat acctaatgta tctatgcaga
     6061 tgcctgagtc cggattttta ggttggataa atgtatcaaa gcttggaaca tcatcagaaa
     6121 tacaaaagta tctagttgac gaggctaaag tttgtattaa tgatggaatt aattatggtc
     6181 caggaggaga agggcatttg agaataattt taggaagcta taaagacgat agtaaggtaa
     6241 ttggtgcatt aaatagaata aaagatgctt taattaaaaa gcaacaataa ataaaaaata
     6301 attggagtgg agtatgttat caatatttaa gagtaaaaaa aatgacaaca agcttaaggc
     6361 atttgcatca gggaaagtaa ttcctataga agatgttgat gataatgttt tttcatctaa
     6421 aatgttaggt gatggaatcg ctatttatcc aactaatgaa tatgttgtag ccccttgtga
     6481 tgcagaagta aatataataa tgaaagatac taaacatgca gttggaatta aattatttaa
     6541 tggtatggaa atcttatttc atgttggaat agatactgta tctatgaatg gagaaggttt
     6601 tgagatattt gtttcaccag gtgacaagat aaaaagaggt gataaattaa tatcttttaa
     6661 tagtaaatta ataaaagaag aagggttaca agatgtagtt ataatggtag ttactaattc
     6721 agaacagtat aaaaatataa aatatcatat tggactggag gcagtaatcg atgatactat
     6781 tattgcagaa atttaaaaac agttatttaa gtaaatactt agataattta gatatagtta
     6841 aaattaaatc cttaaaatca aattatttaa tttttgtgta attttaacta taaagccgat
     6901 taataatata aagtatacta aataatatgg ttattatgat tgttactgac aaagcctaag
     6961 tagatataac aatttgctct aattgagtat tgctatatct acttgttttt atatttaaaa
     7021 taaaagagtc tgcattgaaa agataattct gatgagagta aatagggtat ttaataatag
     7081 tttaattctg acttatcaag atgaacctat tcatatacct aaaacatctt taacaccttc
     7141 taaattcata caaatagctg tataggctgc cttattgatt atttttccgt caatttttac
     7201 tttgtagtga acagcatata taaatattat cgaatagatt gtatcaagag ctctattttg
     7261 ctattcatta gctaaagata gtactctgtc agttattttt gaaactaatg catgagatat
     7321 atcaattccg taaatattat ttaaatgtat gatatatatc gcgagtcgac attccttttg
     7381 agtacatgcc aattacttgg ttctctattc cagaaatttc attttcgtaa ttttgaataa
     7441 ccctaggctg gaaatcatcc ctagggatat ttaattttac ctctccaaaa tctgatttaa
     7501 cattcttagc tctatatcca tttcttgaat ttgatgttgc tttactttgg ttatcatatc
     7561 tctcgtacat taaatgatct tcaatctctg actctaacat tttgtgaatt gtgcttgcga
     7621 ataaatcttt taacatctct tggatgtctt tagtattttt tacatcatac tcttgaacta
     7681 agtgttttaa tatttaattt ttgttgtaca taaaaaatgt gtacctccta ttttggtaat
     7741 tagatttaat atcttgactt ttaggagaat acacatttta ttttacagac cctcttttct
     7801 agaaaagttt ttatgaaaat tgtgatataa attattaata aaaaataatt ttatatttat
     7861 tttattatgt aatttagttt tat
//
LOCUS       DAMJVI010000137         7778 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.142, whole genome shotgun sequence.
ACCESSION   DAMJVI010000137 DAMJVI010000000
VERSION     DAMJVI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.142"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            964..2322
                     /locus_tag="PC545_003309"
     CDS             964..2322
                     /locus_tag="PC545_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HDF2458030.1"
                     /translation="MKAKSTSKFELDGIPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALVFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVASKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNPINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLAFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKVVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLEKFPKFIETLLADSSVVSGAMVAIIIQALYMVKFPGNKSDKVEDKNK
                     SL"
     gene            2429..3223
                     /locus_tag="PC545_003310"
     CDS             2429..3223
                     /locus_tag="PC545_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HDF2458031.1"
                     /translation="MVTIKEYSVPQSLEEAYKILISKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDKKNILIGADTSLRSLEIDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGILELSEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSVLNSAMLKENDTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            3216..5522
                     /locus_tag="PC545_003311"
     CDS             3216..5522
                     /locus_tag="PC545_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDF2458032.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDTVRFIGDE
                     VALVAAEDEKTALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     KNNIVSKEFFEFGDVEAKLKESDFVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFAIDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTNGKAAEDEILSCELRQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGMLKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPIFDKD
                     GRLLTDSFLQYKVPTKKEVGNILVDFAENFDPGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRLRELPMTFEKVYMGMKNNNKQL"
     gene            5661..6938
                     /locus_tag="PC545_003312"
     CDS             5661..6938
                     /locus_tag="PC545_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2458033.1"
                     /translation="MSNRIKNIHYGTQRQQLKNLTKKEYQALREMCFLAKNLYNVGTY
                     NVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSSFRSFFGLIKLAK
                     EGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGILNVPMSPAFKKLYGKVSIKVPQN
                     ILEKKIKEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENLCTCVTNLG
                     DSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQVKDYVNKT
                     CFYIIDYCVKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKLEYLCKIKN
                     INFIRQEESYTSKSDFFADDFLPDINVDNVKKYDFKGERITRGQYKSSVGIIINADVN
                     ASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            7121..>7778
                     /gene="yqeC"
                     /locus_tag="PC545_003313"
     CDS             7121..>7778
                     /gene="yqeC"
                     /locus_tag="PC545_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDF2458034.1"
                     /translation="MELSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIIITIDGTVIPSICHGITVCGSYINNENKLVSIDSSILDEIVDQFDLV
                     LIEGDGSKRKKLKGWNAKEPVVYHKTTKTIGILDITSFGMNINEENIHRVEIFKKIAN
                     LDTSSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLVENI
                     K"
BASE COUNT         3056 a          842 c         1451 g         2429 t
ORIGIN      
        1 atagatagaa acttgtatat tttatgttgc agatacaaat atagtaaagg agagtacatt
       61 ggtacaactg agatacatag cttattaagt ttttctgtct tatgagaatt atattataaa
      121 atattcataa atattttgag tttaaattaa ataattattt tatgcttaga ttaattgata
      181 tttaattaat ttaataacaa aaacagtgaa agagtaaata atattattta cagggggtaa
      241 agcaaagtat gttaaataaa ttgtactttt ggccccgtac atagagaaat ctatgtagga
      301 cacaacttta aaaccagtaa atcctaaagc cttgcaacta caataaaaga tagaaagaaa
      361 cttttatgaa tgtggtgaaa accagaaaca atagtgagga tggtatatgg tgagagccta
      421 agtacttaaa aatggacatt tggtcgccaa gcctagaaat aggaaggtca aacgactatc
      481 tcgagagaga gtacactata agcgattgat agtggaaacg ggttgctctt agcaggtaat
      541 gctgaagatg aagaaatagt ctacgctcat atgaaagtat gaggtcactg gatttgaagt
      601 gacatctata aagtagcgtt tataggtaaa tgtttaaaac ttgaaacatg taaatactta
      661 tgaaaactta taacttataa tgattaagat tgaaagattt aaattgaatt aaatttacta
      721 taaaaaatta aaaattcata gctataatac tcttatattt gtaaagcatg gtatataatg
      781 tatacaaaat taaagaatac gtttatttgt taataattaa atttattgca tctgtttttt
      841 aagactaaaa acttaaatat atgttggagt atagttgata tttatgaaca tcattaattt
      901 aatatattag atttttaagt atttatataa ttcaataagt tttatgtaac gggagttttt
      961 attatgaaag caaaaagtac atcaaaattt gaattagacg gaataccacc actaaaggaa
     1021 gcaatacctt tagggttaca acacttaatg gcaatggtaa taggtagttg tttaccagcg
     1081 atattgattg caaatgcagc tggacttaat cataagatgt caacattatt gattcaagcc
     1141 gcattagttt ttgcaggatt atcaacttta ttacaattat atccaatacc tttgtttaaa
     1201 ggttttaaat taggaggagg aataccagtt ataatgggag ctacagctat gtttatagga
     1261 gctggggtat ctgttgcaag taagtatgga ctaccagtat tatttggctc tcaaataata
     1321 gctggtatag taataattat aattggtttt ggtcttaaaa aaatacgatt tatatttact
     1381 ccagctgtat caggaacaat agttgcatgt atgggtcttg gactttttcc aatagctata
     1441 aaaaacttag ctgggggtat gggcaatgag acttttggga atccaattaa ttttatagtt
     1501 ggaatatctg tatctcttat gatattagga ttaaataagt atggaaaagg attatttaaa
     1561 gatgcatcaa tattagttag cataattttt ggatacattt tatcattaat tttgggtata
     1621 gtaaattttt cttcaattca ggagtttact ttggttgcac ttccaaaacc acttgctttt
     1681 gggctagata taagacttga agttgttgta atgttttcaa taatatattt agttgagata
     1741 gctgatataa tgggtgcttg tacactttct gcggtaggag ggcttaatag acaagtaaca
     1801 gatgaagaac tttcttctgc tgttttagga aatggagtac tctctacaat aggtgcatta
     1861 ttttctgcaa ctccaatggg gatgtttgga caaaattctg caatcgtttc caatacaaag
     1921 gttgtaagta aatttgtact agctattgga ggaattggat tatttttggc aggaatatcg
     1981 cctttattag ctaatttaat aagaacaatt cctccctgtg tggttggagg agcaacactc
     2041 gttatatttt caactcttac aacatctggt ttaagacttg ttagtatgga tggatttaat
     2101 caagaaaata gcatgatatt aggtctttct atggcttctg gtattggatt catggtagct
     2161 cctcaagttc ttgaaaaatt tccaaaattt atagagacat tacttgcaga ttcaagtgta
     2221 gtttcaggag caatggtagc cataataatt caagctttat atatggtaaa atttcctgga
     2281 aataaaagtg ataaggttga ggataaaaat aaaagtttat aatgtaaagg tttaatatat
     2341 gtgaattaaa tatactacaa ttataaatat aaataaaaga tttattaaaa acttttgtgt
     2401 tttaataagt taatgatggg gggataagat ggttactata aaggaatatt cagttccaca
     2461 gagtttagaa gaagcatata aaattttaat ttctaaaaaa aataatgtta ttttaggtgg
     2521 atgtggattt ataaaattaa gtaataaaaa tataggaaca gcgatagatt taaaagatat
     2581 aaatctaaat tatataaaag aggataaaaa aaatattttg ataggtgcag atacttcact
     2641 tagaagttta gaaattgata aaactataaa aaattactgt agtggaatac tttcaagcgc
     2701 tgtttctaat atagttggtg ttcaatttag acaaggggct agaattggtg caagtgtatt
     2761 ttcaaagtat ggcttttctg acttaatacc agcgcttttg gttgttggag cgaaggtgag
     2821 attatacaat aaaggaatac tagagttaag tgagtttttg gatagtgaat taagtaggga
     2881 tatattggtc gaagtaatac taccaaaaaa agatgcaata gctgtttttg actcaataag
     2941 aaaatgcaca ggagattttt ctgtattaaa ttcagcaatg ctaaaagaaa atgatactta
     3001 caaaatagca attggggcaa gacctcaaaa agctagatta gcacttgaag cgagtaatat
     3061 attaaataaa gaaaaagata tagataaggc atctattgtt gctagtaagg aacttactta
     3121 tggaagtaat atgagagcta gtaaagagta cagaaaggat atggcaagtg cattagttaa
     3181 aaggatgtat aatgctattg aggagggaat gtataatgat taataagggg ataagaaaga
     3241 tagatagtac agcaattgtc acaggaaaac ctctatatac tgaagacctc attatgcata
     3301 aagatgtttt gacattaaaa ctacttagaa gtccacatgc tcatgctaag ataaagaata
     3361 tagatacatc aaaagccgaa aaaattccag gggttgtggg tatttatacg tataaagatg
     3421 taccacaggt cagatattgt gtgccaggtc aaacagaacc agaagcatca ccttatgata
     3481 gagttttact tgaagataca gttagattta ttggagatga agtagcttta gtggctgctg
     3541 aagatgagaa aacggcactt aaagccttaa aaatgataaa ggtagattat gaggttttaa
     3601 agcctgtgtt tgatgcaaga gaagcagaag gaaatgaagt tgttatacat ccagaaaaag
     3661 acatagtaga gccatatcct tttggattgg atgcaaaaaa taatatagta tcaaaagaat
     3721 tttttgaatt tggagatgtt gaagctaagc ttaaagaaag tgattttgtg gttgaatctt
     3781 catacaaaac ccaaagtcaa tcgcattgca tgatggaaac tcataggtca tattcatata
     3841 aagatataaa tgatagaatt gttgtagttt catcattaca atcaccacac aatgtaaaga
     3901 gaatacttag aaaagtattg ggtataccat cttcaaaaat aagagtaatt aaacctagaa
     3961 taggtggtgg atttggagga aagaatatag cagcaacaga tatatttact gcatttgtta
     4021 ctatgaaaac tggtagacca gcaaaatttg tatttacaag aagtgaaact tttgtaggtt
     4081 ctaatagtag acatgaaatg ttttttgatg ttaaagttgg tgcagataaa gaaggaaatt
     4141 taaaagcaat agagctaaag tcattaaaca atacaggtgc ttatggtggg aatggtgtct
     4201 ctgtttcttc cgaatctgga cacaatacct taccaatata taatgatatt gatgctgtta
     4261 gatttaatgc tagaacagta tatactaata ggttaccagc aggagcactt agagggtatg
     4321 gtgctacaca gggaatattt gcaatagata gtgctattga tgaattggca cataagattg
     4381 gaatggatcc acttgaattt agaattaaaa atataataga taggtatacc aatggaaaag
     4441 cagctgaaga cgaaatatta agttgtgagc tgagacaatg tatagaaaag ggcaagaaat
     4501 taattaattg ggatgataaa tatccatgta aagatatagg aaacaacaaa gtaagagcag
     4561 taggtatggc agtagcaaat catggttctg gtataccgcg tgtagatatg gcaacagtca
     4621 ctataagaat ggatgaagat ggcacatata aactattatc tggtcttgct gacttaggtc
     4681 aaggggctga tacaattcaa gctcaaattg ctgcggaagc tttaaatact actgtagata
     4741 gaatttctat ttatacaggt gattcagact tatgtcccta tgatacagga gcatttgctt
     4801 cttgtacgac aacggttaca ggaaatgcaa caataaagac agcaaataaa ttaaaagata
     4861 tattactttc agttgcagag tcaaagctag gatttaaaaa agaagattta gagttgggaa
     4921 aagacagggt ttttgttaaa aacgataata gcaagtttgt tctattagag gaattgggta
     4981 gagaatctgt tggaggaata ggatttgcta gtgatgatgt accagtagtt ttggaagcaa
     5041 cagaatcttt tggcatgttg aaaaaaataa aaccatttat agcaggattt gcagagatag
     5101 aattagataa aaatacaggc gaatataaag tcataaattt tgcatgtgta cctgattgtg
     5161 gaacagttat aaatccaact ttagctagaa tacaagtaga aggtggtgtg atgatgggta
     5221 tagggtttgc aatgtatgaa gaccctatat ttgataaaga tggtagatta ttaacagata
     5281 gtttcctaca gtataaagtt cctacaaaaa aagaagttgg aaatatattg gtagattttg
     5341 ctgagaattt cgacccagga ggtccttatg gtgctaaatc attaggagaa atagttatac
     5401 atacaccagc accagctata gcaaatgcaa tatttaatgc tacaggagtt cgtttaagag
     5461 agcttcctat gacttttgaa aaagtttata tgggtatgaa aaataataat aaacaattat
     5521 aataaataat aatgtgtata aaagcaagta aatttaaatt attttgtaaa ataaagtaat
     5581 taaatggaca ttatatatgg tgttttgtaa aaataccgta tataatgtct agtagagtat
     5641 attctaaagg aggtgagttc atgtcaaaca gaataaaaaa tatacactat ggaactcaga
     5701 gacaacaatt aaaaaactta actaaaaaag aatatcaagc tctacgagag atgtgcttct
     5761 tagctaagaa cttatataat gttggtacat ataatgtaag acagcattat ttcaatcaag
     5821 gagaacatct taactatgaa gctaattatc atgtatgcaa agaaaatgag aactataagc
     5881 ttctcaatag taatatagcg caacaaatat taaaagaagt agacagttcg tttaggtcat
     5941 tttttggatt aattaaatta gccaaagaag gaaaatatga ctttagaggg ataaaatcac
     6001 ctaaatattt agataaagaa ggatttttta gcataataat aggtcaaata agaatcaaag
     6061 aagatgggat tttaaatgta cctatgtctc cagcatttaa aaaactatat gggaaagtaa
     6121 gtataaaagt tccacaaaat atactagaga aaaaaattaa agaaataaga ataataccta
     6181 aacataaagc taggttcttt gaaattcagt attgttatga aataccaaaa tcagaggaag
     6241 tttcaaatca aaaaaatgca ctagctatag acttgggttt agaaaattta tgtacatgtg
     6301 ttaccaattt aggtgatagt tttatcatag atggaaaaag gctaaaatct gtgaatcagt
     6361 gggcaaacaa acagaatgat aaaatagaag ccttaaaatt aaaaaagaat ataactgcaa
     6421 taacaagaaa acaatttaaa gtttggaata agagaaataa tcaagttaaa gactatgtaa
     6481 ataaaacctg tttttatata attgattatt gtgtaaaaaa taatattgga aatttaattg
     6541 taggatataa tgctaagtta gaaaaaagtg ataatatgga gagaaaaata tatagaaatt
     6601 ttataagaat cccatttgga gagataaaag gaaaattaga atatctgtgt aaaataaaaa
     6661 atatcaattt tattaggcaa gaagaaagct atactagtaa atcagatttt tttgctgatg
     6721 attttctacc tgatatcaat gtggacaatg ttaaaaaata tgactttaaa ggggaaagaa
     6781 taacgcgtgg acagtacaaa tccagtgttg gaatcattat aaatgcagat gtaaatgcga
     6841 gtcttaatat actaaaaaaa tcacaagtag ttaatttatc taatttacag aataatcctc
     6901 aaatattaaa gcaaccacag agagtaagaa tatcttaatt atatttaaaa gataaactga
     6961 tagcgtaagc ttacagctta gcggcgaatt ggatccgcct tgttgtttac aacagaatct
     7021 atttactttc gagtttatgg aatttcaaaa atcactttta ctaaaaaggt ctaactataa
     7081 taattaagag attagaaaac tggttataag aggtgaaagt gtggaactat caaatataat
     7141 agaaaagaat gaaataataa ctgtagttgg agcaggagga aaaacttcgt ttataaacta
     7201 ttttgcaaat ttttatagag ataaattgaa agttctcctt acaacaacaa ctaaaatata
     7261 tgtaccaaac gattatgaca atataattat tacaatagat ggaactgtta ttccatctat
     7321 ttgtcatgga ataacagtct gtggcagtta tataaataat gaaaataaat tagtaagtat
     7381 tgattcctct attttggatg aaattgtaga ccaatttgat ttagttttaa tagagggaga
     7441 tggctcaaag agaaaaaaac ttaaaggttg gaatgctaaa gaaccagtag tatatcataa
     7501 aactacaaaa accataggga tattagatat aacatcattt ggaatgaata ttaatgaaga
     7561 gaatatacat agagtagaaa tatttaaaaa aatagcaaat ttagatacaa gtagtattaa
     7621 tagtagttct acagtaagta ttgaaaactt aaaaaatata gtattaaatc caaatggatt
     7681 atttaaaaat tattctggaa aaagagtatt atttattaat aaagttgaaa atgaaaaata
     7741 taaaaattta gctataaaat tagtagaaaa tattaaag
//
LOCUS       DAMJVI010000138         7773 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.143, whole genome shotgun sequence.
ACCESSION   DAMJVI010000138 DAMJVI010000000
VERSION     DAMJVI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.143"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            367..1188
                     /gene="yidA"
                     /locus_tag="PC545_003314"
     CDS             367..1188
                     /gene="yidA"
                     /locus_tag="PC545_003314"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDF2458035.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMSLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNNIEVKYIQKDKIDESIKICKIMIVDEP
                     ERLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCTNLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            complement(1325..1474)
                     /locus_tag="PC545_003315"
     CDS             complement(1325..1474)
                     /locus_tag="PC545_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458036.1"
                     /translation="MNFFWTKSDFDAWTNEAGLSNDEDIYCLDINEAIVESYKIFKLK
                     QKVLS"
     gene            complement(1481..1966)
                     /locus_tag="PC545_003316"
     CDS             complement(1481..1966)
                     /locus_tag="PC545_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458037.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNIEKEYIERTLQYFIYKNIMVVDGNSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDIKINSICSATGKEIEVIIKNEKIEYVNNPNLRVLHINLDKHLNWAAS
                     C"
     gene            complement(1979..2731)
                     /locus_tag="PC545_003317"
     CDS             complement(1979..2731)
                     /locus_tag="PC545_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458038.1"
                     /translation="MKLSVRDINKTFVNNRVHTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSERGDVFINDKRVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEVLLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            complement(2731..2868)
                     /locus_tag="PC545_003318"
     CDS             complement(2731..2868)
                     /locus_tag="PC545_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458039.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            complement(2908..3537)
                     /locus_tag="PC545_003319"
     CDS             complement(2908..3537)
                     /locus_tag="PC545_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458040.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFMYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIVPASLPDILTGSRIAISTGWMSVI"
     gene            complement(3498..4061)
                     /locus_tag="PC545_003320"
     CDS             complement(3498..4061)
                     /locus_tag="PC545_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458041.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPEKIGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            complement(4071..4295)
                     /locus_tag="PC545_003321"
     CDS             complement(4071..4295)
                     /locus_tag="PC545_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458042.1"
                     /translation="MENMKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            complement(4300..5391)
                     /locus_tag="PC545_003322"
     CDS             complement(4300..5391)
                     /locus_tag="PC545_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2458043.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKPKEKEEAKTGKDDYTIT
                     VGYYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGA
                     IPKGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLP
                     TDPSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKAN
                     GKEYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEV
                     ALRTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGA
                     DDFDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            complement(5426..5884)
                     /locus_tag="PC545_003323"
     CDS             complement(5426..5884)
                     /locus_tag="PC545_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458044.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            complement(5877..6962)
                     /locus_tag="PC545_003324"
     CDS             complement(5877..6962)
                     /locus_tag="PC545_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDF2458045.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGPKVTIIYLIAGFLVPFLVGFLGNCFGKEELSLHNDSEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAALVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            complement(6989..7174)
                     /locus_tag="PC545_003325"
     CDS             complement(6989..7174)
                     /locus_tag="PC545_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458046.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIITKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            complement(7336..7668)
                     /locus_tag="PC545_003326"
     CDS             complement(7336..7668)
                     /locus_tag="PC545_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDF2458047.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPVGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
BASE COUNT         2561 a         1363 c          859 g         2990 t
ORIGIN      
        1 ataacatatg attaatggaa ttacaatatt ttataacaca aaactaacta atataacaaa
       61 ttaccatcag tctatttatt caaacttcta actatataat atacatttat ttatattaac
      121 aatgcaaacg ataccattat ttgtaattaa gaattttgga attattgatt acaaagtttt
      181 tacattctca tctcacataa gtaatatttt tatcaaaaaa ttaatattat taaactttct
      241 aaattactta tctcttgata taatagtata caaaacaaac catatttccc actgaaactt
      301 tgttcaacac tagctgtgtc aacgttttca caggaaattt gagtatataa tttattggag
      361 gttgatatgt acaaattaat cgcactagac attgatggaa caatattaaa tacacaaaaa
      421 aggattactc ctgaagtctt tgagtccatt caggaggcaa aaagggctgg ggcaaaagta
      481 gttataacaa caggcagacc tcttcctggt gtaaaagaac ttctaaatca actaaattta
      541 acagatgaag gtgattatgt tatatgcttt aatggagcaa ttattcaaga agttaaaagt
      601 gagaaaatca tacatgatgt agaaatgagt ttagatgatt ttgattttat atataataac
      661 gtttgtaaaa aatataagac taaaatacac ataaatacaa tgacaaattt aattacacca
      721 aatgaaacgc ctggcaaata tacacttcat gaagcgaaat taaataatat agaagtcaaa
      781 tatatacaaa aagataaaat tgatgaatct attaaaatct gtaaaattat gatagttgat
      841 gaaccagaaa gattggaaga aatcatacag caactaccta aaaatttatt caataaatat
      901 actatagtaa ggtctgctcc tttttatcta gaatttttag gtaaaactac taataaagga
      961 actgctctta aaacattatg tacgaatcta aatataccta tagaaaatgc tattgctgtt
     1021 ggcgatgaag aaaatgacca acatatgata aagtatgcag gtcttggtgt ggctatgggg
     1081 aatgcacgta atagtataaa ggaaattgca gattatgtta ctgatactaa taatgaaaat
     1141 ggagttgcaa aggttataaa taaatatata cttaataagg ctatctaatc atctaaaaaa
     1201 aactaaaaat atacctttag atttaaatac ttttcacatc cctactctat actaatttat
     1261 atagtatgga tattacctat catttttatc taaaggtcat ttttagtttt tcatatatgt
     1321 atatctatga tagtaccttt tgtttgagtt taaatatttt atatgattct actattgcct
     1381 cattaatatc taaacaatat atatcttcat cattagataa tcccgcttcg tttgtccaag
     1441 catcaaaatc actcttcgtc cagaagaaat tcattatatt tcagcaacta gcagcccaat
     1501 ttagatgctt atcaagattt atatgaagca ctcttaagtt tggattattc acatactcaa
     1561 tcttttcatt ttttataatt acttctattt ccttacctgt agcactacaa atagagttta
     1621 tttttatatc ttggttgaat gtgcaagagg ttcctatagc atctatagca cacattgcac
     1681 tgaaacttct attatctctc aaagttactt tatgatttgt tggcaatgct gatacaggat
     1741 atataaaatt tatactattc ccatcaacaa ccattatatt tttataaata aaatattgta
     1801 gtgtcctttc tatatattct ttttctatat tcaacttttt acttatatat tcagtcactt
     1861 cttgaagtgt aacactagaa cctttatcta ttatcatatc cataatagag attcttattt
     1921 tctgttgtgt attatccaaa ctattgtatt tttctatttg actcatgtct actcctcttt
     1981 aaacttcata acactttgat aaagtatcag ttaatttgtt tttgtactct tcatattttt
     2041 ggcttcctct aattctaggt cttggaatat ctatatttat atcatcaata attcttcctt
     2101 tatctctcga cattactata actctatcac tcatatatac agcttcatca acatcatgag
     2161 ttaccattaa tgcagttatt ttcttcttta tccatattcg ttctagctct tcctgtaaat
     2221 tttgtctcat ttgaaaatct acagctccta atggttcatc cattagtaat acctctggat
     2281 tacatgctaa agctcttatc atggccactc tttgtttcat tcctccagat aattgatgcg
     2341 gaaacagttt ttcttttcct ttcaagtctg acataaccaa aagttcttct agcatttctt
     2401 ctcttttttc ttttggtatt ttctgctgtt tcataggaaa tagaacatta cctcttatag
     2461 ttttccaagg aaataaagca taattttgaa atataaaagc tctatctatg cccggttttt
     2521 taactctttt atcatttata aaaacatctc cacgttctga tttttgaaac ccacctatta
     2581 tagtaagtaa agttgttttt ccacaacctg atggtcctaa aagacagaca aattgtccat
     2641 catctatatc catacttata tcttctaaaa cctttgtatg tactctatta tttacaaatg
     2701 tcttatttat atctcttaca cttaacttca ttaatctgca aacctccatc tacaaaggat
     2761 tttatttata aactttaata ccctatctat tataaatcct attaaagctg caaatatcat
     2821 aagcccaatt aatacatctg tatgcattaa tgtttgagct tgtaccattg agtaaccgag
     2881 accggcactc gtagctataa actcggctca gataacagac atccatcctg tacttatagc
     2941 aatccttgaa ccagttagta tatctggaag agatgcggga acaattacat ttgtaaatat
     3001 cacaattggc gatgctccca tacttctagc tgcattataa taatcctttg atattgctct
     3061 tactccttga atagtattta aaattatagg gaaaacccct gaaaaagcta ttaaaaatat
     3121 tgttggacca tctcctatac caaaccatac tatagtaaga ggaacccatg ccattattgg
     3181 aacttgcctt acagaatcta ctactggaga taaaaccctt tcaaaagttt tagaaaatcc
     3241 cataataaat cctattggaa gcccaaacaa taatgcatac ataaaacctt taagtactct
     3301 ctgcaatgtt ataaaaagat tagtgactgt ctctgcatct gtcacacaga aaaagaaacc
     3361 ttttattaca tctactggca ttggaagtaa caaagaactc cctatatctt ttgctgcaaa
     3421 atgccagact aaaaatataa taactaatag tattatttta tataaaatat ccatactacc
     3481 atcagttttt ttcttagtta atctatcgct tttatcttta gaaattttaa tttccaattg
     3541 cttatttcct ccatatctat atttcttata ttcctgtact cttttaatgc tttcttaaat
     3601 gtagctttat tctttaaagc atcaacactt ttattgtaaa gtgttataaa atcactatat
     3661 gctttacttt ttttaaactc tttacttaca actaatacat aggtatcata atctcctaat
     3721 ttagttgtag attttctttt gccctctaac ccaattgact ttatgcaatc tattacaaca
     3781 ccatctacta aattactttc taatccatat gctagtgcag tccctaataa gggagctact
     3841 tccacattct tgtagtattt tttaaccaat tctgactggt aatctctatt ttgaatgact
     3901 cctatctttt caggattatt atcacttaat agaaatatat cagaattttg aactacagta
     3961 ccaacaattt caaatccact atttacattt acatattgtt ttgcagcatc tttacatatg
     4021 attgctatat ctatttcctc tgaactcaga gcccactgca ttgtacttgc tcagcaatca
     4081 ttaattgtaa aaatatccat tatatcactt acttttatat tttcaaagta gtctctattt
     4141 accatataat ttattacaaa accagaggta tcatcagata cccctattct taattttcct
     4201 tttatttctt ctttatcttc aaaattaaaa aatattataa atatacttcc caaaataatg
     4261 ataaaaagta ttattatatt tttcatattc tccatttatt tataatacct ttgctttcca
     4321 gtcttcataa ctcatacctt caggaaactc tgggttaact ttttctttta taaatttgtc
     4381 aaaatcatca gctccacaag agtccaataa gcttgtatca atcgtatctt catatttagg
     4441 tatatcatct aaagccttta aatctttata catttgtaaa ttatttttat attcttcacc
     4501 tgtaactttc caagtaagag ttctaccttc tccaacagtc ttcttataga tagttctaag
     4561 tgcaacttct tcttctacat tataatattt tgcaaatatt ttagctgctt cagctggatt
     4621 tgtatatata tattctattg cttttgtatg tgctagaacc aatttttttg ctaaatctgg
     4681 atgttctttt ataaagtttt tgtttaatga gaatgaacaa cagttatatt ctttgccatt
     4741 agctttttct ttatattgag tagatgctat tatttttcct gcaccgtcat tttcagctac
     4801 agaaccccaa gggtcacaag atgtaaaagc ttgaattttc ccagttttaa gagctaaata
     4861 tttatctttg tctgaatcca tatttacaac ttcatatttt gatgggtcag ttggtagacc
     4921 tgtttctgga ccataatcac ttgtccataa gaaatcactc atatctgttg caattttttt
     4981 acctattaaa tctttaggtt cttttatatc ttttgaaact attaaatatt cagaaccacc
     5041 agtatggttg ttagctgcta ttgttattgg tgaaccctta ggtattgctc caactaaccc
     5101 cttagttcct atgtaacctg catccatttt tcctgctgcc atagcttcag gaactttacc
     5161 atttccaaca gtttttacat taagacctaa atctttatat attcctgccg attctgctac
     5221 tggtcccgca gtcatatggt cacaattata ataacctact gttattgtat aatcatcttt
     5281 tccagttttt gcttcttctt tttcttttgg tttactacta catcctgtta atatagatgt
     5341 acccattaca ccaaataaga ttaatgcttg aatcttctta gataatttca tataaatttc
     5401 ccacctttat gtattatttt tatttttagc aacatttgtc catatcttcg ccaaataaat
     5461 caacaaattt tttaccctct actctgtaaa tagcatatcc tacattacca tttttagacc
     5521 ctgatacaat tacttctatt ggtgctttat catcaatatt tttaaaggtt atagtttgat
     5581 tctctatagg tgctggtatt ggctttgtta tatagtggct atttttatca gaaactacga
     5641 ctaccatttc atttgaatta ttgctttttt tatatataac tactaaatct tttttcccat
     5701 cattatttaa gtcttcttca gcacaagtta ttatgtcttc tttataattt tttctaaaat
     5761 attctaatgt ttctttattc acatttggaa tagaagcatc ttctttgatg ttatcatcaa
     5821 ctatttttga taaaatgatt aacacaacta ctatgcagga tattataact atctttttaa
     5881 gcatgatttc ctatgacctc tttcatcttt ggtattattg atttaaatgc aaataaaata
     5941 attaaaatag tacatacata ctcaaaacta tcaggtatat ttaacattag tttatttgcc
     6001 aactgtattg aagaatttgt tgcatctaaa tttactgagt tattattcac taaaagtaaa
     6061 tttgttatat atccaattat tattgaagac agtgtcaaag ttacagaata tataataaca
     6121 gttctttttc caataagttt aaacatactt ataagttctg gtagatttgt agctgctcca
     6181 gctattagaa atgttatcgc tataccagga gatgctccac ttgcaacaag ggctgctata
     6241 aacggtatat gtcctacagc acatacatac ataacacatg caagtattgc tatacttcca
     6301 agagatagca aacctggatt tcctaaatat ttttgaatta tactatttgg aaaaatagtt
     6361 gttataaatc ctgcaaaaac catacccatt actacatatt tactaatagt aagggctaat
     6421 tcaccaaaag accatttaag accatcaatc atattttcca taaaacttct tttttcttct
     6481 tctaattcta ttacttcttc ctctgaatca ttatgaagtg ataattcttc ttttccaaaa
     6541 caatttccta aaaatcctac taaaaatggt actaaaaaac cagctattag ataaatgatt
     6601 gtaactttag gacctaaaag acctagacaa agaactacag ctattggatt tattattgga
     6661 gttgatgtca taaaagcaag tactggtcct aaatatgctc ctgaataata catacttatt
     6721 ccaagtggta ttactccaca actacatatt ggtaaaaaca ttcctgagat agtactttta
     6781 attaaagaag aaaatttctt atttccaagt tgtttttgaa accttcttgg agttaaaaca
     6841 ttgtgaagta cacctgccaa gataaaactt atcataagcc atcctgatgc tccattcagt
     6901 gtaactatag aagctactat agtatcttta actccattta aaacaatttc taatatattc
     6961 atttttgata ctcctaattt ttaaactttt aattattgtt tatagcttct actattgccc
     7021 tttcaattgt atctttattt aatctatcat atttcttttt ctgatttatt attaaagttc
     7081 cttttgttat aatcccatat ttttttatat acgaaaaatc tttccctgct tgatataatt
     7141 ttatttccaa tttatcagca tacttttctg acattttttt tataaacact ccatgttcat
     7201 cacaagatgg tcaagtgttt ataaattcaa ctaaaatttt agacatttgt tcatccccta
     7261 ctttaaacaa tttatctttt acttaaaata atccacagaa aataaatttt tctatggatt
     7321 gtatcctata ctaatttata acgttataac ttggtccaca cctgcactgc ctaaacaagc
     7381 atataggttt gggaaacatc caattgtagc tccatcaacc aaattattct ctatacctct
     7441 ttctatagca cattggtcac aagccattat tatcatatca gttttttcat gtaatttact
     7501 tagtctctcg cctacaggat tacctttaga taaaaataag gtattatcca taaagaaaaa
     7561 catccctaga acgtctactc catgagttcc ttgttcaagt tgtggtatta tcattttgtc
     7621 tagaatttta tgtgtattag tactactaaa aatataagct actttcattt ttattccccc
     7681 tatacttttt atattttaca aaattttaca gatacaatta tattataagt ttattcttat
     7741 ttatctgtaa aatagatatt aactatatta tta
//
LOCUS       DAMJVI010000139         7767 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.144, whole genome shotgun sequence.
ACCESSION   DAMJVI010000139 DAMJVI010000000
VERSION     DAMJVI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.144"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            306..1499
                     /gene="nifV"
                     /locus_tag="PC545_003327"
     CDS             306..1499
                     /gene="nifV"
                     /locus_tag="PC545_003327"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HDF2458048.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1524..3449
                     /locus_tag="PC545_003328"
     CDS             1524..3449
                     /locus_tag="PC545_003328"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HDF2458049.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREEAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIGGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKDNNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLGSGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3471..4466
                     /locus_tag="PC545_003329"
     CDS             3471..4466
                     /locus_tag="PC545_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HDF2458050.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHGVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIV"
     gene            4792..5265
                     /locus_tag="PC545_003330"
     CDS             4792..5265
                     /locus_tag="PC545_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2458051.1"
                     /translation="MVVSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNMLKVDKATTTKAIQKLIKAGYVDKKQDKFDKRGYNLT
                     PTDKSLEVYELIIEEENRSIEICFDNFTDEEKQVVTKLLEKMSKNVENEWFKVKR"
     gene            5299..5793
                     /locus_tag="PC545_003331"
     CDS             5299..5793
                     /locus_tag="PC545_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2458052.1"
                     /translation="MIRKANMNDLESIMQIIKSTVEEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKTGI
                     ASKLVDFAEKLAVENNVSYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5890..6411
                     /locus_tag="PC545_003332"
     CDS             5890..6411
                     /locus_tag="PC545_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2458053.1"
                     /translation="MIGFLKKRRSIRKYKDVEVEKEKLDKILKAALLAPSSKGLRTWE
                     FIVVDDKEKLINLSQCRTKGGGFFLKNAPLAIVIIADKEKNDVWIEDASIAASYIQLQ
                     AHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPSKYSVECIISIGYPDEEKKAYGD
                     SDLDYKKVHFNNF"
     gene            6600..6911
                     /locus_tag="PC545_003333"
     CDS             6600..6911
                     /locus_tag="PC545_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDF2458054.1"
                     /translation="MGIFKTKMDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKSLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            6939..7625
                     /locus_tag="PC545_003334"
     CDS             6939..7625
                     /locus_tag="PC545_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2458055.1"
                     /translation="MKYSYKKCIIDSILLMFIVQILRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNNILIEKCKLILFVVVLSLA
                     IIVTYCTKGYVLFNVTMMTLSVLIVPIFEELFFREYIWNYLSNFIKSKSKIICITSIL
                     SGIYNIGYIDVIRNYVILYNNSSYTFEVIISKIMIGTVFGIVLGLVKYRFRDVGFCIL
                     LRSLFAIFIR"
BASE COUNT         3117 a          761 c         1498 g         2391 t
ORIGIN      
        1 gtaggaattt tataggttta tttttaaagg ttttttgaaa ataaatctat ttaaaaatta
       61 aattaaaatg caagaaaaac ataataactt tcatttttaa gaagttttca caaaaaagaa
      121 aaaatatagt gcaataaatg atacaaagta atataataga ttacaagata tgaaaagcta
      181 aaaaaataaa ttgcaaatga aattaaaatg aataaaaaat aaaaaaataa tcataagtta
      241 gaggtatcaa tgaaaaataa gacttaatgt tttaaaatta aaagtatact aaggggggca
      301 ttaatatgtg cgttatatca aaagatagag caaaggaaat aaaaatagta gatactactt
      361 taagagatgg agagcaaaca gctggggttg tatttgcaaa tagagaaaaa ataatgattg
      421 ctgaaatgtt aagtgaccta ggtgttgacc aaattgaagt tggtatacct actatgggtg
      481 gagatgaaaa aaatgtaata aagcacatat gtagtagaaa tttaaaatca gatataatgg
      541 cttggaatag agcagtaata aaagatgttg aagaatctat ttcttgtggt gttgatgcag
      601 tagctatatc aatatcagta tctgacattc atatagaaaa taaattgaga acttctagag
      661 gctgggtttt agaaaatatg gctaaaacag ttgaatttgc aaagaaaaat ggattatatg
      721 tttcagtaaa tggagaagat gcttcaagag cagatataga cttcttaact gaatttataa
      781 atgttggaaa acaagcaggg gcagatagat ttagatactg tgatacagta ggagttatga
      841 atcctttttc tataaagaat gcaatagaaa ctctttatga aagaactaat tttgatatag
      901 aaatgcatac acataatgac tttgggatgg caactgcaaa tgcacttgca ggaatagcag
      961 caggagcaaa ttatgttggt gttacagtaa atggattagg tgaaagagca ggaaatgcag
     1021 cattagaaga agttttaatg gcacttaagt gtgtttataa atgtgattta aataatatag
     1081 atactagaaa atttagagga atatgtgagt atgtagcaca ggcatcagga agaatacttc
     1141 ctacatggaa accagtagtt ggggataata tgtttataca tgaatctggt atacatgcag
     1201 atggagcttt aaaagaccca cataactatg aaccatttga cccaagtgaa gtaaatcttg
     1261 aaagaaaaat agtaataggt aaacattcag gaagagctgc agttgtaaat aagttgagtg
     1321 aatatgaaat gtatatttct ccagaaaacg ctaccaagct attaaatgct ataagagcta
     1381 catctataag attaaaaaga agtttaatgg ataaagaaat tcttcaatta tattgtgata
     1441 tactagcaca tgagaaaggc acaacagaag aagaagcagt tagaggttca tatatttaag
     1501 atataattgt ttaggaggaa aatatgggag ataatatagt ttataaaata attaaaaaac
     1561 atatagtaga tggagaagct gttgctggaa gttcaattgg tataaaaata gaccaaactt
     1621 taacacaaga ttctactgga acaatgacat atcttcaact agaagctatg ggaatagata
     1681 aagtaaagac taaaagaagt gtggcatttg tagaccataa tatgttacaa caaggatttg
     1741 aaaatgcaga cgaccataag tatatacaaa cagtggcaga taaatatgga gtttatttct
     1801 ctaagccagg aaatggtatt tgtcatcaag tatttttaga aagattttca acaccaggag
     1861 atacactttt aggttctgat agccacacac ctactgctgg aggtgtgggc atgatggcta
     1921 ttggagctgg tggattagat gttgctttag ccatggcagg aggagcatac tatataaaag
     1981 caccaaaagt ctgtaaggtg aatctagttg gaaaattaaa taatatggta tcatcaaaag
     2041 atataatatt agaagtgtta agaaaacaaa ctgtaaaagg tggagtaggt aaagtttatg
     2101 aatatggtgg agaaggtgtc aaaagcctat ctgttccaca aagagctaca ataacaaata
     2161 tgggggctga acttggagca actacttcaa tcttcccaag tgatgaaaaa acactagaat
     2221 tttttaagag tcaaggaaga gaagaggcat ggatagagct aaaaccagat gcagatgcag
     2281 tttatgatga agagattact ataaacttgg atgaattaaa accattagct gcaaaacctc
     2341 atagcccaga caatgtagat gaagttgaaa atataggcaa gattaaaata gaccaagtgg
     2401 caataggaag ttgtacaaat tcttcttatg aagacttaat gaaagtagca caaatcctga
     2461 aaggaaataa agtacataag gatgtgagtt tggtaatagc accaggttca agacaagtta
     2521 tggagatgat tgctagaaat ggggctttgg cagatataat aagtgctgga gctagaatac
     2581 tagaaaattc ttgtggtcca tgtattggta tgggtcaatc tccaggtact gactctgtgt
     2641 ctttaagaac tttcaataga aatttctatg gtagaagtgg gactttgtca gcacaagtat
     2701 atttagtaag tccagaagtg gcagcagtaa gtgcaatcaa gggagtgctt acagacccaa
     2761 gagaatttga tattaaattt acaaatttag atgtaaatga atttttgata gatgatagca
     2821 tgataattaa acctgctgat gtaggaagtg atgtagaagt agttagagga cctaatataa
     2881 aacctttccc tcttaataca gagctaagtc agtctatagg tggaaaagtt atattaaaga
     2941 cagaagataa tataactact gaccatataa tgccatcaaa tgctaagtta cttcctttta
     3001 gaagtaatat accgtatctt gcaaactatt gtttcaatac tgtagataca gaatttcctc
     3061 agagagcaaa agacaataat ggtggattta tagttggtgg agataattat ggtcaaggtt
     3121 caagtagaga acatgcagct ttagcaccat tatatctagg tgttaaaggg gtaatagtta
     3181 agagctttgc aagaatccac aaggctaact tgataaatag tggtataatt ccaatggagt
     3241 tttgtgatga gaaagattat gaaaatatat ctcttttaga taatctggag ataccaaata
     3301 ttttagacaa tctaggtagt ggaattttag aagttaagaa tacaactaaa ggaacttctt
     3361 ttaaggtcaa agtagaattg tcagcaaaag aagttgatgt attaaaagct ggtggaaaat
     3421 taaactacac taaaaatcaa gctaattaat atgaaaagag ggaatattga atgtataaag
     3481 tgacacttat accaggagat ggaataggac cagaagttgc gaaagctatg aaaaaggtag
     3541 tagaagctac tggagtagaa atagaatggg aagaagttaa tgcaggagag gcagttattg
     3601 aagagtatgg tactccactt cctgaatata ttatagatag tataaaaaag aacaaaatag
     3661 ctataaaagg cccaattact actcctgtag gtaaaggatt taggagtgta aatgtaactt
     3721 taagacaggc attagatttg tatgtcaatt taagacctat taaaagtttt aaggggataa
     3781 aatcaagata tgaagatgtt gatttagtgg ttgtaagaga aaatacagaa gatttatatg
     3841 ctggaataga acataaaata ggtgattatg cagctgaaag tataaagata ataacaagaa
     3901 gtgcaagtga aagaatcgtt gattttgcat gtaactatgt taaagataat aagagaaaaa
     3961 aagttacagc tatacataag gcaaatataa tgaaaatgtc tgatggattg tttttagatg
     4021 tgtttagaga agttgcatca aaacatggtg tagaatatga tgatttgatt gtagatgcag
     4081 cagctatgaa cttagtttta aatccagaaa attatgatgt aatggtaatg ccaaatcttt
     4141 atggagatat actatcagat ttaggtgctg ggcttgttgg aggtttaggt ataattccaa
     4201 gtgcaaatat tggtaaagat tgtgcgatat ttgaagctgt tcatggaagt gctccacaaa
     4261 tagcaggtca aaataaggct aatccaacag cacttattca atcatcagta atgatgctta
     4321 gatacttagg tgaatatgaa aatgcacaaa aaatagaaac agctttagaa aaagtatttc
     4381 ttgaaggaag taaattgact gtagatttgg gtggctcagc ttctactaca gagtttgcgg
     4441 atgaggtgtg taagtatatt gtatagattt tatttaggga aaaagtaaaa tatagattga
     4501 aggaaagagg ctttctcaaa atagagataa gcctttttta ttggaatgat aaaatatatt
     4561 tgattttata caaaagagag ttcctcataa acgaaaaagt taattttggg atatccattt
     4621 taattttaaa attaattgat taatatgcaa agaaagatta aataaattta ttatttttaa
     4681 ttgaactttc aaccaaaata tggtaaaatt taaaaaagtt gaactttcaa ctaaaaatga
     4741 atatattgat tatgtgaaat tttataaaac agaaactatt tgactttagc catagtagtt
     4801 tcagtggagg tgtgtttgat taagacttta gatagtaata tattaagaga agtaggtact
     4861 ttatctagag cagtaaattc aataaatgat attaagtaca aggaattaaa gttacaaaaa
     4921 ggacaattta catttttgac aagaatctgt gaaaatcctg gtataaatct tgtggaatta
     4981 tcaaatatgt taaaggtaga caaggctaca acaaccaagg ctatacaaaa attaataaag
     5041 gcaggatatg tagataaaaa gcaggataag tttgataaaa gaggatataa tttaactcca
     5101 actgataaat ctttggaagt ttatgagttg ataattgaag aagagaatag aagtatagag
     5161 atatgttttg ataattttac agatgaagaa aaacaagtgg taactaagtt attagaaaaa
     5221 atgagcaaaa acgtagaaaa tgaatggttt aaagtaaaga gatagtaaat aaatacatta
     5281 acttaaggag atatttttat gattagaaaa gcgaatatga atgatttaga aagcattatg
     5341 caaattataa aatctacagt ggaagaaatg aagacttata ataatacaca atgggatgaa
     5401 aactatccat tagaaaaaga ctttgtttca gatattaaga aacaagattt atatatttat
     5461 gaagtggatg gagaagtagc agggtttata tgtcttaatt atgaagagcc agaagaatat
     5521 ttaggcttaa attggtcatc taataaaaaa gcaatggtaa tacatagaat ggctgtaaat
     5581 ccaaacttta gaaagacagg aatagcatct aagttagttg attttgctga aaaattagct
     5641 gtagaaaaca atgtatcata cttaaagagc gatacatatt caataaactc aaaaatgaat
     5701 ttattactta caaagtgtgg ttttataaaa atcggagaaa tgagtttttt aggaaaggag
     5761 aaatctttct actgttatga taaaatatta taaagagcat ttataataca agggataaat
     5821 aatagatttt cagcattagt aagggcataa tataacctag tgtatgctat tatgaaaggg
     5881 atgatttata tgattggatt tctaaagaaa agaagaagta ttagaaaata caaggatgtt
     5941 gaagtagaaa aagaaaaatt agataaaata ttaaaagctg ctcttttagc tccatcatca
     6001 aaggggctta gaacatggga atttatagtg gtagatgata aagaaaagct aattaattta
     6061 tctcaatgta gaactaaagg tggtggattt ttcttaaaaa atgcaccttt agctattgta
     6121 attattgcag ataaagaaaa aaatgatgtt tggatagagg atgcaagtat agctgcttca
     6181 tacatacaac ttcaagctca tgaactaggt ttaggttctt gttggataca agtaagaaat
     6241 agaatgcatg atgataatat agaagctgat aagtacatta gagaagaatt aaaagtacct
     6301 agcaagtaca gtgtagaatg tataatttct ataggatatc ctgatgaaga aaagaaagca
     6361 tacggtgact ctgatttaga ctataaaaag gttcatttta acaactttta gtatataata
     6421 tgttgtatta agacattata tttttttcta ttgttaatat atacttaaaa ttgtaaaaat
     6481 atatctgaat tttactaact tatctagggg ttatgtacaa ggattttaaa gataaatata
     6541 gaaattaatg tttataatcg atacataact tgagtaacaa aatttaagga ggttaaagta
     6601 tgggtatatt taaaacaaaa atggatgagg actggaaagt aaactacatt aaagagttta
     6661 atgaaatgag agattcttat gagagtaaac ttcaaaaaaa acagtttgag gtagatagtt
     6721 tgaaatcaga attggataga ctaagaagct ataaaaatag ccttaagcca aaagaaaaac
     6781 aaattactga tgatgatata aataatataa agagtttaag aagagatggc ttaagctata
     6841 aagaaatttc aaatcaaaca agttggagca aagcaactgt aagtagggta ttaaatggac
     6901 tttacgatta gcatatacat ggggtgattt aaattgggat gaagtatagt tacaaaaaat
     6961 gcataataga ttctatttta ttgatgttta tagttcaaat attaaggatg attttgaatt
     7021 atgtacttct aagtcaattt gaatttactc ttgaaaattt taatattata aacttaattt
     7081 cttttacatt agttggttta tcgttaattt tatttttaaa agataattct ttgtataaca
     7141 aggttagaaa tagaaaaatc acagaagctt ttgaagaaaa taaaaacaat atacttattg
     7201 aaaagtgtaa gctgatactt tttgttgtgg ttttatcttt agcaataatt gtaacttatt
     7261 gtactaaggg atatgtactt tttaatgtaa ctatgatgac tttgtctgta ttgatagtcc
     7321 caatatttga agagttattt tttagagaat atatatggaa ctatctaagt aattttataa
     7381 agtcaaagag taaaattatc tgtataacat ctatactatc tggaatttat aatataggat
     7441 acatagatgt tataagaaat tatgtaatat tgtataacaa ttcaagctac acctttgaag
     7501 ttattatttc aaaaattatg ataggtactg tttttggtat tgtacttgga ttagttaagt
     7561 ataggtttag agatgtaggg ttttgtatat tattgaggag cttatttgct atatttatta
     7621 ggtagatagt ataatcggtt tataaggttg ttttaaattt aattttaaat caaccttatt
     7681 ttatttgtgt tttataaagc acttatatat taataactaa taaatttaat aaaaattatt
     7741 tgaatatttt taaaatatat aaatatg
//
LOCUS       DAMJVI010000140         7755 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.145, whole genome shotgun sequence.
ACCESSION   DAMJVI010000140 DAMJVI010000000
VERSION     DAMJVI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7755
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.145"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..1332)
                     /locus_tag="PC545_003335"
     CDS             complement(<1..1332)
                     /locus_tag="PC545_003335"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDF2458056.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMIEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSKTGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFKIAEGTKQSIHKKADLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGYILNKMLEIVINNPELNEKEILKEKALNIYT"
     gene            complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="PC545_003336"
     CDS             complement(1481..3286)
                     /gene="lepA"
                     /locus_tag="PC545_003336"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDF2458057.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDDRRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            complement(3568..3819)
                     /locus_tag="PC545_003337"
     CDS             complement(3568..3819)
                     /locus_tag="PC545_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458058.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRILFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPSIKFDITKFDFDIPNFDIHEIIKKFNK"
     gene            complement(3850..4869)
                     /locus_tag="PC545_003338"
     CDS             complement(3850..4869)
                     /locus_tag="PC545_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HDF2458059.1"
                     /translation="MFKKCIKVVTLTFILVCILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTENKKNNKNKETSKENKEESKEGNTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNTTSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            complement(4887..5840)
                     /locus_tag="PC545_003339"
     CDS             complement(4887..5840)
                     /locus_tag="PC545_003339"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HDF2458060.1"
                     /translation="MISVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDRQGAEIMNKDIGKYITLESNLMKFDDDESREEMISYLKEELVDIFGQDKNKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            complement(6182..6958)
                     /locus_tag="PC545_003340"
     CDS             complement(6182..6958)
                     /locus_tag="PC545_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2458061.1"
                     /translation="MKFHEFGNKNRPHIMLIHGGGNAWWNYLRQAEVLSNKYHVILPT
                     LDGHGEEYETTYISTEDTADKLISYIDENCSGHLFALCGVSLGGQIVMELLSRKPNIT
                     KKAIIDGSICYPRPLMARFCIAGIRFFGGLLFSEKACRFQIAMMPKLLPENMQYPDEI
                     KAYYMTDMPHVRKETFYNMYRTYMMNYTLKENVKKTTAQVMYWYGEKEMKCVKKSAKL
                     FQSYVPSCEIYEAKSYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            complement(6992..7513)
                     /locus_tag="PC545_003341"
     CDS             complement(6992..7513)
                     /locus_tag="PC545_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458062.1"
                     /translation="MDIAKLILNPARLRILQYIRLYGSVRTSDIVKYLNDIPRATVYH
                     HVKILEENNMIEVIKENRVRGTIEKVYALKEYTTSMEGETFVALSTAFYVGLMQEMNE
                     YFSRKEQDHKKDNVFFSSALLYMSDNEYENLLKSIADLLKPYIEQKPKSNLKLRKLSI
                     ISSPPVADNDKTK"
BASE COUNT         2396 a         1387 c          870 g         3102 t
ORIGIN      
        1 tgtatatata tttaatgctt tttcttttag tatttctttt tcatttaact ctggattatt
       61 tatgactatt tcaagcatct tatttaaaat atatcctata tctttacctg gttttatacc
      121 tatagatatc aaatcttttc cattaatagc taaatcttta agattaaagc actccttttg
      181 atttattata tgtttaaatt ctttttcaca ctcaataaga tttaatatct tttgttttgc
      241 atactttgga ttttttgcaa gtgtatcagc ccaattaact tttattaaat cttcaaataa
      301 ttcaacatct tcaagtttat ttaataactt tttaattatc ttccttttag gttctattct
      361 ataatcatga tattgtatta atattaaaac tttattaata gtataattat catacttaag
      421 tcttttcaaa atcttctttg caatctttac agattctcta ctatgagtat aaaaatggtc
      481 aacaccattt ttatcagttg ttttagttac ttttttacct aaatcatgaa acaacattgt
      541 aagttttaaa tacagttcat cttcaattac ttctgtagct attaaagtat gtttttgtac
      601 atcatagatg tgatatggat tgtgttgagt aacatcttct aattcacata tttcaggaat
      661 tataaatttc aacaaacctg ttgattttaa taagtttaat ttagatgaat cagatactaa
      721 tattttatta aactctgtct ggattctttc aatagaaaca ttttttatca aatctgcttt
      781 tttatgaata ctttgcttag taccttcagc tattttaaaa ttgagttgtg ctgaaaatct
      841 aactgctcta agcattctaa gtgcatcttc ttcaaatcta tctactggat ttccaacaca
      901 acgtatatac ttattttgta tatcttcata gccattaaat gggtcgacta atcctgtctt
      961 tgaattgtat gcaattgaat tgatagtaaa atctcttctt gacaagtcct taactatgtt
     1021 atttgtaaat tctattgaat ctggatgtct accatcacta taatttccat caattctata
     1081 agtcgtcact tcaaatggtt catgttcaat cataactgtt acagtcccat gttgtatacc
     1141 agttggaatt gttttttcaa ataattctac taccacttct ggtctagcac tagttgttat
     1201 atcccaatcg tttggaactc tttctaataa acaatctcta acacatcctc caactatata
     1261 agcttcataa ccattctttt ctaattcttt aattatatag tctacttttt taggaatttc
     1321 tatattaatc ataaatatca cctttttatt cttctttaat attcttactt atattctcta
     1381 tgagtttact taaaatatac cataaaaaag ctagactaat ttagaaataa ttctaaaatc
     1441 actctagctt ttaaatatct ttatctaaat ttataaggat ttactcatct aatttaagca
     1501 cagacataaa tgctttttga ggaacttcaa cagaaccaat ttgtctcata cgcttcttac
     1561 cttctttttg tttttcaaga agtttcttct tacgagatat atctccacca taacatttag
     1621 caagaacatc ttttcttaga gcgcttattg tttctcttgc aataacctta ttaccaacag
     1681 cagcttgtat tggtatagca aactgatgtc ttggaatttc tccttttaat ttctcacaca
     1741 ttgcctttcc tctagggaaa gcacgtgtct catggacaat aaaactaagt gcatccactt
     1801 gctctttatt gattaatata tcaagtttta caagtgtaga tgctacataa cctttaacct
     1861 cataatcaag agaaccataa cctctagttc ttgattttaa agcatcaaag aaatcgtaaa
     1921 caacttcatt gagaggaagg tcatagtgaa gcattactct tctatcatct atatattcca
     1981 tatttaacat atttccacgt ctttcctggc aaagctccat aacaacaccc acatagtctt
     2041 taggaactat tatatctcct tttactatag gctcttctat catctttatc tcagatggtt
     2101 ctggtaagtt tgcaggattt tgtatcatta caacctctcc atccattttt gtaactctat
     2161 atataacaga tggagctgta gtaattatat caaggttaaa ttctctctca agtctttctt
     2221 gcattatctc catatgtaaa agacctaaga aaccacatct aaaaccaaat cctagtgctg
     2281 ctgaagtctc agcttcatat tcaagtgcag catcatttac ttgaagcttt tcaagagcat
     2341 ctctaacatt ctcatacttt tctccttctc ctggatatat accacaatat accattggag
     2401 tagctttttt gtatcctggt aaaggttctt ctgtaggatt atttgaatct gttattgtat
     2461 caccaacacg acaacttctt atatctttta tactagcagc aatataacca acatcacctg
     2521 ctgataattc actaagttca gtttgacctg gagccataac accaacttca gttacctcaa
     2581 actttttatt ggtattcatc atttttattg tcataccctt tttaacagtt ccttcaaata
     2641 ctctaacata agctacaaca cctttgtaag catcatagta agaatcgaat attaatgcct
     2701 ttaatggagc ttcattatct cctttaggtg gaggaacatt ttttacaata tcttctaaaa
     2761 catcttctat gtttaaacca gttttagctg aaataagagg agcttcactt gaatcaagcc
     2821 ctattaaatc ttctatttca ttttttactt cctctggtct tgcacttggt aaatctattt
     2881 tatttattat tggtaatatt tctaaatctt ggtctatagc aagataaaca ttggctaaag
     2941 tttgtgcctc aacaccttga gctgcatcaa ctactaatag tgctccttca catgcagcta
     3001 aacttcttga tacctcatag ttaaagtcta catgtccagg tgtatctata agatttaaat
     3061 aatattcatt tccatccttc gctttataca ttaatcttat gttttgaagc tttatagtga
     3121 ttcctctttc tctttcaagg tccatattgt caagtaattg acttttcatg tctctttcac
     3181 taacaagccc agtttgttgt attaatctat cagctaaggt agactttcca tggtctatat
     3241 gtgcaattat actaaaattt ctagttctac tttgtttatt gtccacctta aaattccctc
     3301 ctcttgatta ttcttatagt tattatttta cattatctaa aaactcttta tataccaaat
     3361 ttaaaatttt tgaatataaa ctaattggca ctaaacaaaa ttatccacat tatatgtgga
     3421 ttatcaagtc taacactaaa tattatataa tatcataaac ttaaagtaaa tcctataaaa
     3481 tacagttaat gaatttttat tttatacatt aaaaatatat aactaaaata aggcttattt
     3541 taatcaataa accttattca cttattacta cttattaaat ttttttatga tttcatgtat
     3601 atcaaaattt ggtatgtcaa aatcaaattt tgttatatca aatttaatac ttggaaaatc
     3661 atattgtttc attcctagca tactttttgc attactgtcc actataaata cacaaactga
     3721 tgtacatata atcatagtaa tcaaaaataa gattctcata atatacttgt attttcttag
     3781 tttactttta ctttctatat ttttttctaa tcggctcatt tagcatgctc ctaattataa
     3841 cttaaagctc taattttttt gtttaaaata ttcatccaaa atctcggcta aatactttgt
     3901 agtagcctta gattcttcta cacttgttgc gttattccct atttctacta acatatgatt
     3961 cttaactgca aactgattga atctagcata gtctttttct actattggag atactagacc
     4021 aggatacttt ttctcagcaa aagcagttat atcttctgct aaagctctaa gttgttttct
     4081 atttgtattt ttacctccta caacaaagaa gaactttgat actctctctc catttatcat
     4141 agttgtatat tttaaatggt ctttttcctt agtggctgga ttagttaaat ccctagcatc
     4201 tctatgtaag tctatcgcta tatccactga gttatattta ggtaatatac tttgtattgt
     4261 ttttaagcta cttgcataag agttattata agatggatag tcatggtatt tagtagtatg
     4321 gactacaccc catcctttgc tatctaaagc actagttaat gcacttccta cttccattac
     4381 tgaatttttc ttatctcttg aatgataatt tccatcaggt gaatttgaat aagtctcaca
     4441 tccatgagta tgatatatca aaattcttgg attttctttg gttactctta aatcatcctt
     4501 ataatctgaa ctagatgttg tgttagtttc agttgtgtct gaattttttg actcaatatc
     4561 tggaacattt tctttaccaa cataaagttt tatatattct ttttcactct cttttttatt
     4621 atcaacttta cttgcatctt tagatttagt gtttccttct ttactttctt ccttattttc
     4681 cttacttgtt tctttatttt tattattctt tttattttct gtatcatttc cttcgacttt
     4741 agcttctgga taagatgaat ttactaaaaa ctttaaaaaa tcatcttgat tcaaagctaa
     4801 tgattttcct ggtaaaatgc atactaatat aaaagtcaag gttactactt taatgcattt
     4861 tttaaacata attgctcctc cttattttat accaatctac ctggatgtaa agacctattt
     4921 aagccttcac ttattattat agatagattt tcgattgtat catctatatc ctttggtgtt
     4981 actattaagt tcttgtcata tgggtctaac gaatctttta ttagatgata tttttcttct
     5041 tctttaagtt ttttcaacat ttcataaaaa ctttctgttt cttctgattg agctattaaa
     5101 ttgtctattg ccatatctaa gacatctatt gtaagagttg ctgcatcaac caccgttgga
     5161 acgcctattg caataacatc cacgcctaaa gtttctttag ttagagattt tctagtattg
     5221 ccaacacctc caccaggtga tattcctgct gttgatatct gtatagtaga gtttactctc
     5281 tccatttttc ttgaagccaa tgcatctatt gctactactc tatctggctt aaccttctca
     5341 accaatgatt ttactatttc actagtttct attccagtaa cacccataac tcctgggctt
     5401 aaagctgata cctcagtgaa atcatcatca tagtctttat tataattttt aaaaagatgt
     5461 ctagttacaa gtgtttttga tacactcttt ggtcctaacg catcagaagt aatatttcta
     5521 tttccaagac caattaccag ggttttttta tttttgtctt ggccaaatat atctactaac
     5581 tcctctttta aataacttat catttcttct cttgactcat catcatcaaa cttcataaga
     5641 ttactttcca gagttatata ctttcctata tctttattca ttatctctgc gccttgcctg
     5701 tctattactt caactttagt tacaatgcaa ttttctaatt ctttagtttc tatttttaca
     5761 ccaggaatac tacttccttc ttgagacttt tcacacattt cactagcttc taaagctaaa
     5821 tctgttctta cactaatcat ataaacctcc agtataaatt aaatgctaat tataattgtt
     5881 aacaaaattt agtattttat aacaagaaat tgtaaattta tttaaaacaa gtaaaattaa
     5941 ggctttcaat gtaagaaaat tgggtttagt cagtgatttg tcagtaaacc ttcttatcta
     6001 tcatttattt acaaacagaa aaaggtagca actatttata taggtgctac ctttaatcta
     6061 tttataattt tcctattaag ctttcactaa tatattaact aaaataattt tttatattta
     6121 tacctaattt atttactata taaaaaaaca gcaataatta tacaataatc acctaacacc
     6181 tttattcctt ttggaaaaat ggttctgcaa tttccaacca ttcatttgga agataaagtg
     6241 ataaataccc atgattatag ctcttagctt cataaatctc acaagagggc acataagact
     6301 gaaacagttt tgcagatttt ttgacacact tcatttcttt ttctccatac cagtacatta
     6361 cttgtgctgt ggttttttta acattctctt taagtgtata gttcatcatg taagtacgat
     6421 acatattata gaatgtttct tttcttacat gtggcatatc tgtcatataa tacgctttta
     6481 tttcatctgg atattgcata ttttcaggta acaattttgg catcatagca atttgaaagc
     6541 gacaagcctt ctcactaaac aaaagtcctc caaaaaatcg aataccagca atacaaaacc
     6601 ttgccataag aggtcttggg tagcaaatac taccatcaat aattgctttt ttagtaatat
     6661 taggttttcg agataaaagt tccatgacaa tttgaccacc aagtgatacc ccacacaaag
     6721 cgaaaagatg tccactgcaa ttttcatcta tatatgatat caacttatct gctgtatcct
     6781 ctgtagaaat gtatgttgtt tcatattctt ctccatgacc atccaatgta ggaagtatca
     6841 catgatattt gttagacaaa acttctgctt gtcgcaaata gttccaccat gcattccctc
     6901 ccccatgaat caacataata tgtggtctgt ttttatttcc aaactcatga aatttcatat
     6961 ataacctcct tttataaaaa tgggttgcat gttattttgt tttatcatta tcagctacag
     7021 gtggagaaga tataattgaa agttttctaa gttttagatt tgatttaggt ttttgttcta
     7081 tatatggttt caataaatca gctatacttt taagaagatt ttcatattca ttatcactca
     7141 tatatagtaa tgctgaagag aaaaatacat tatccttttt atggtcttgc tcttttctgc
     7201 taaagtattc attcatttcc tgcatcagac ctacataaaa tgctgtagaa agtgcaacga
     7261 aggtctctcc ttccatagat gttgtatatt cctttagtgc ataaaccttt tcaatagtac
     7321 cacgcacacg attttcttta attacttcaa tcatattgtt ctcttctaaa atttttacat
     7381 ggtgataaac tgttgcacga ggaatatcat ttaaatactt tacaatatca gatgtacgaa
     7441 cactgccata aagtcgaata tattgtaaaa tacgaagcct tgcaggattt aaaattagtt
     7501 ttgctatgtc catcttatca gccctttctt tagtctataa aaatattata gtctattttt
     7561 ttagactatt caagtctatg atataaatag taattacaac tataataaat gctacaaatg
     7621 ctattctata taaacactga cattaattac atataacctt ctaacatatt agttatattg
     7681 tttgatgcat ctatcataca tactaatgaa aaaaattatc ttgattattg gaaaaattat
     7741 cttaattaag catat
//
LOCUS       DAMJVI010000141         7742 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.146, whole genome shotgun sequence.
ACCESSION   DAMJVI010000141 DAMJVI010000000
VERSION     DAMJVI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.146"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(234..2513)
                     /locus_tag="PC545_003342"
     CDS             complement(234..2513)
                     /locus_tag="PC545_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDF2458063.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEESFEVDNILSILKRQAINSSFKRM
                     GVILPNGTAYTTDGYIEDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSNINVYSV
                     PIYKDNEVKGVIFATHSTKLYEKILSVPTFNGKGYSYITRSNGDIVLNPSSKNANSTK
                     ENLFSYIGRSDSISKSSINNMKSNIKNFKSGSLSYSMFGKGYYLSYAPIGINDWYLFS
                     EVPKTAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRESIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTTVKRKYLKVYEIYAENLKETLIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGVFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPGFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRFIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSLNRKETN"
     gene            complement(2784..4238)
                     /locus_tag="PC545_003343"
     CDS             complement(2784..4238)
                     /locus_tag="PC545_003343"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDF2458064.1"
                     /translation="MKLNDIIQGLDIINIKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTKADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAIAEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            4402..5271
                     /locus_tag="PC545_003344"
     CDS             4402..5271
                     /locus_tag="PC545_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458065.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKDIQFEYHNHDFNKIIIFINGNVT
                     YFVDGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDASSDLEK
                     CFKIATENKCNRLRLNIHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENMESTEDVFYDETIKKVLNYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NNKNPLDITLLDE"
     gene            complement(5484..7556)
                     /locus_tag="PC545_003345"
     CDS             complement(5484..7556)
                     /locus_tag="PC545_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2458066.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMVDNSMYANIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAIMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDESETNAEVIEMSKNELASEILIALGNKDNLKSLDACITR
                     LRLVVNDINKVDKEKLKSLGAAGVMVIGNNVQAIFGPKSDSIKSDIKDIIEGKTKVES
                     TVTKNVDKNKVKKNMEVANMENALVEKIDSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSSDGLIYSPVDGTIGVIFPTKHAIIIKSQKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGVSTAKEENRVSIL
                     RDDIEKIG"
BASE COUNT         2623 a         1291 c          825 g         3003 t
ORIGIN      
        1 attactatta ttactatcta aagtattttg ctataatccc atatactctt acctcaaaaa
       61 cattcaaatg taaaaaatac tacatattaa taaactttgc ccaataattc ctaaaataat
      121 atcctaacaa tgatttatgt ttttataata gtatattttt acataaaaaa gtgaacattg
      181 attaacaaag ttcacttttt tataaatttt tatgttataa ttcacaattt tatttaattt
      241 gtttcttttc tatttaaact atatctatct aatattaatt tttcatattc ttctattggc
      301 attggtctag caaataaaaa tccttgggcc atatcacaac ctataaacct taaaaactct
      361 gcttgctcta cagtttcaac accttctgaa acaactttta tatcaagttg ctttgccatt
      421 tgaacaatac ttgaaactac aatttgactc ttttttgcat ttcccactgt tataaagaat
      481 tgcctatcta atttcaatac atcaattggc atatctttaa gcatatttaa agaagaataa
      541 ccagaaccaa aatcatccat agaaatttta aatccaacag atttcaaagt ttgcattatt
      601 ttaaataata tttttgtatt gtcaaatatt gcactttctg tcaattcaat ctcaattaaa
      661 tttggttcaa tttcatattc ttttgttatt ctttcaagct cagaaataaa gcctggattc
      721 tcaagatgta ctcttgataa gtttattgat ataggaaatg tggggaaatt ttcattttcc
      781 caacgtttaa aatttttaca aacttgggta aatacaaaca tatccaattc tgttataaag
      841 ccatttttct caaaaatagg aataaataca cctggactta ttagaccttt tttaggatgt
      901 tgccaacgta caagtgcttc agcacctaca attttagtag ttgataaatc aaccttaggc
      961 tgtaaataaa ctttaaattg ttcatttttt aaagcatcat gcatttcttg ttcaatatca
     1021 cgttcttcta tgagagtttc ttttaaattt tcagcataaa tctcataaac ttttaaatat
     1081 tttcttttta ctgtagtttt tgcaatcata gctttgtcaa gacaggtaga aatatttgtt
     1141 tctccctctt caacaaaata tacacctatg gatggaatta aattgagttc tactcccaaa
     1201 cttgttttta tcattgacaa ttttttacgt ataagttcac atatttctat aattgactct
     1261 ctattatttt tcttttcaaa tattatagca aaattgtcat ttgaaattct tgaaaataca
     1321 gattgctctt taaatatatt cttaattatc tctgctattt cctttaatac tctatctcct
     1381 tcttcatacc caaacctatc atttactagc ttaaacttat ctatatcaaa ataaattaaa
     1441 actagattct ttttctcatg agaggataaa aattttcccc cttcaagatt aaatttattt
     1501 gaattgccaa tacctgtaac gttatcttta aaagcaaatt cttccaattt ttccttactc
     1561 tttttatgta taaatagtat ataaattgtt aatgccgtaa agattataat caagactaca
     1621 catgctgata atgtaagttt tataatagca tatgatttct cagatatagc agttttagga
     1681 acttcagaaa aaagatacca atcattgatt cctattgggg cataacttaa ataatatcct
     1741 tttccaaaca tactataact taatgaacct gatttaaagt tttttatatt tgacttcata
     1801 ttgttgatac tagattttga tattgaatca cttctgccaa tgtaagaaaa taaattttct
     1861 ttagtagagt tggcgttttt actgcttgga tttaaaacaa tatcaccatt acttctagtt
     1921 atataagaat atccttttcc attaaatgta ggtacagaaa gtattttttc atacaacttc
     1981 gtactatgtg tggcaaatat aactccttta acctcattat ctttataaat tggtacagaa
     2041 tatacattaa tattactatt ggtattttta tcatcttcta ttacatcttt taatttgcct
     2101 gttataacca cttctccttg cattgatttt aagaaataat ctcttttaga gaagtcttct
     2161 atataaccat ctgtagtgta tgctgtacca tttggtaaaa taactcccat tcttttaaat
     2221 gaactattaa tagcttgtct ttttagtata gaaaggatat tatctacttc aaaagactct
     2281 tctccttcaa taaatattga aattgactct agtaacttta tatctccatt aatttgtttt
     2341 tttaacacat tgacactttg atttgctatt tctgaaagat attgattcgt ttcttcacct
     2401 aatgtcttat tcaaccttga accataaatc aaacaggaaa ttgtaattac taagatactt
     2461 atgacagcaa ttatggacaa accataccta ttattttttg catcacttaa cattatgctt
     2521 cctgcctttc tcgaatctat gtatgtataa tcattatctt atgacaatta ttgtaaacat
     2581 tttataataa tacttatttt tccatattta ctgtaattat acaaaattat aaactttatt
     2641 acaacaataa attaattaat atagatatta gtgtttatat tattgcatta tctattttac
     2701 aattatttac atataaaaaa atgtaatatc aaagtgaagc ttgatattac atttttgttc
     2761 ttgtattttg tttaattttt atgctacatc ttacttaaac actcatttgc aacttcttta
     2821 tcatcaaaat gatgcttaac tttacctata atttgataag tttcatgtcc cttacctgtt
     2881 ataattacaa catcatcctt ttttgccata cttatagctt ctgctatagc ttgttcccta
     2941 tcaacaacaa catggtaatt ttcttttttc ttgtcaattc cttcaagtat atcttttatt
     3001 attgcctctg gttcttcagt tcttggatta tcagaagtta taatacataa atcagaatat
     3061 ttttgtccaa ttgctcccat caaaggtctc ttttcagtat ctctatctcc accacaccca
     3121 aacacagaaa ttatttttcc ttctgcaaat tgttgagttg tctttagtac attttcaagt
     3181 gcgtcaggag tatgagcata gtctacaatt acgcttatac ctttatcatt tggaactgtt
     3241 tcaaaccttc ctgatactcc attagaaagt ctaaggcctt ctttatatat tggtttaggt
     3301 atgccaagta catagcaagc agctataact gctaaagtat tatatactgt aaacattcct
     3361 ggaactggta taaatattac ttcttcatat gaaggtgtta ttaatctata agatactcca
     3421 tccgaatctg attttatatc tctagccata aaatcagcct tagtatcaac accataagtg
     3481 taacaaggta catttatacc ttttatattt tcatatatct tttttccacc ttcatcatct
     3541 atgtttatta tatttgccat agtagtctta aaaaataatt tttcttttgc ttttctataa
     3601 tcctctaaat ctttatgaaa atctaaatgg tctggtgtta aatttgtaaa tatgcctagt
     3661 ttaaagtctg tttcatcaac tctatttaat gccaatgaat gtgatgaaac ttccatcgca
     3721 cagtagtcac atccattatc caacatatta ttaaaatgat attgtaaatc tatactttct
     3781 ggagttgttg aattggaaac aatgtctttt tctccatcaa aaatttttat agttcctata
     3841 agtccaactt tattcttatt taaagttaat atctcattta gtatagtagt tatactagtt
     3901 tttccattag tacctgtaac tcctataacg ttaaattttt gagatggatg attgtaaaaa
     3961 ttatcagcta cttttgccat atcttttctt gtatctttta cctttataaa tgtacatcct
     4021 tttatttcta catcttcttc aacaagaaat gcacttgctc ccttttctat ggcatctttt
     4081 atatacttat gaccatctga cacaaatcct tttatacata taaataatgt accttttgta
     4141 acctttcttg aatcatattg tacattatta atatctatat ttaattcacc cttgatattg
     4201 attatatcca acccctgtat aatatcattt aatttcataa atgctttcct ccctttaaac
     4261 tctttctgct aaaattacac tacatataat attatattaa gaatttttta ctatgtgaat
     4321 ggttaaaaat gcttgaaaca ttgcaaaaaa tgctctataa ttttctataa tataactgag
     4381 gaaaggtctt ctataaaaac tatggataat aaaatcgggt acttaaacga taactttaaa
     4441 atatttaata ttagagataa aaaagatata caatttgaat accataatca tgattttaat
     4501 aagataatta tatttataaa tggaaatgta acttattttg ttgatggtat tccctataaa
     4561 ctaaagcctt gggatatact ctttatcagc aataatgaaa tacataaacc agttatagat
     4621 tcaaatgtct tttatgagag aattgcaata tggattagcc cagaatttat gaaaaaaaat
     4681 agtgatgcta gtagtgactt agaaaaatgt tttaaaatag ctacagagaa taaatgcaat
     4741 agattgcgtt taaatataca tgatattgaa tctattaaga gttttattga gcaaataaat
     4801 atttccgaat ccaatactga atttggaaat gaaatattga gaaatgcatt atttatacaa
     4861 cttatgatat tcttaaacag aattttccta aaaaatgaaa atatggaatc aacagaagat
     4921 gttttttatg atgaaactat taagaaagta ttaaactata taaattcaaa tatagatgat
     4981 aagctttcaa tagatagcat atcgtctgaa ttttttataa gtaagtatta tcttatgaga
     5041 aaatttaaat ctcatactgg aacgtctata cacaattata tagttcaaaa aagattaatt
     5101 ttggcaaagt cactaataac caatggtcac cttatgagtg aagtttgtaa taaatgtgga
     5161 ttcaacgatt attctagttt tgtaagggca tttaaaaaaa actacggggt atctcctaaa
     5221 aattatataa acaataaaaa tcctttggat attactcttt tagatgaata gataatttat
     5281 gtaaatttta ttttaataaa acttgaagta ttagtgtatt tatcttacta atacctcaag
     5341 ttttttaatg ttttaaaata tatcaaaata taatgatata tatttctatt acttaaaata
     5401 tatattaaat aaattgcatg ttaactgaac ctatttatat atttattaaa tcccacataa
     5461 cttttagtta cactatcata tagctatcct attttttcaa tatcatctct taatatactt
     5521 actctattct cctcttttgc tgtgcttact ccttttttaa tgcttaattt ctgattttgt
     5581 tctaaattcg taaatactac aggacttatt gttgatagac catttttttc aatatattct
     5641 aagtccattt taactaattt ttctcccgct tttacttcat cccctacatt cacaaatact
     5701 tcaaatccat ttccttctaa atttacagtt tctatcccta aatgtataag tatttctact
     5761 ccactttttt gggattttat tattattgca tgcttagttg gaaatataac tccaatagtt
     5821 ccatcaactg gtgaataaat caatccatca ctagatttaa ttgcaaatcc atcccccatc
     5881 agtttacttg aaaatacaga atctggcact tcttctatat ctaatatctc tccatttgtt
     5941 aatgaatcaa tcttttctac taaagcattt tccatatttg caacttccat attttttttc
     6001 actttatttt tatcaacatt tttagtaaca gtactttcaa cctttgtttt tccctctatt
     6061 atatctttta tatcagactt tattgaatcc gattttggtc caaatatagc ttgaacattg
     6121 ttgccaataa ccataacacc tgctgctcca agtgatttca atttttcttt atctacttta
     6181 tttatatcat ttacaaccaa tctaagtctt gttatacacg catctaggct ctttaaattg
     6241 tctttatttc ctaaagcaat caaaatctct gatgctaatt cattcttaga catttcaatg
     6301 acttcagcgt tagtttctga ctcatcttct cttcctggtg tctttaaatt aaattttctt
     6361 atcataaatc taaatacact atagtataat actgcaaaac caatacctac caagatagcc
     6421 aagtaccatg gagttctatt aggaagaaca ccaaataata agaaatctat taatcctcct
     6481 gaaaatgtga atccaatttt aactcctaat aattgcataa ttgcgtatga taaacctgaa
     6541 aataaacaat gtattgcaaa taatactggt gcaacaaata aaaacgcaaa ctctattggc
     6601 tcggttattc ctgttaaaaa agaggttaat gctgctgatg ccattatacc tgctacaaat
     6661 tttttctttt ctggctttgc ttcatgcacc atagcaagag ctgctgctgg aagaccaaac
     6721 atcataaatg gaaatgcacc tgtcataaaa gtacctgctg tgaaatttac tccatcttta
     6781 agttgagcaa agaatatctt ttggtcaccc attacaagct caccagcttt atttacatat
     6841 tctccaaatt gataccaaaa tggattatgg aaaacatgat gcaaaccaaa tggtattaat
     6901 agtctttcca aagaaccttc cataaatgct gctagtgttt gatttgcttc tataacattt
     6961 tttgagaaac taagtaatcc ctcttgtatt ggaggccaaa taaaagacaa tataattcct
     7021 attgctatag caactaatga agtaacgatt ggtacaaatc ttttacctgc aaaaaaacct
     7081 agatactgag gtaattcaat tttataaaac tttttataaa gataagcagc cactatccct
     7141 ataataatgc caccaaatac ccctgtttga attgttggta ttccaacaat atttgcatac
     7201 atagaattgt ctaccatatc ttctgtaaca cctgccataa cacccataga tgtattgaga
     7261 attaaaaaac ctactatggc tgccaatgct gcaacacctt ctccttcagt taaaccaaca
     7321 gctaccccaa ctgcaaatat aagtggtaag ttattaaata taatttgacc tgaactttcc
     7381 attatactta ttataagttg aaatgtagga ttcgccaaag ctggaaaatg agccaacaat
     7441 gcgtctcctg ctaaagcatt actcacgcca agtaatatac ctgctgcagg aagtaatgca
     7501 actggtagca ttagagattt tcctactttt tgtaaaacac caaatacttt tttcatttac
     7561 ttttctctcc ttattatttt atattttttt aaataaaaaa gacttaagtt tataaaaagt
     7621 tacattagta tacttaattc taaaattaaa cttatgttaa tataaataaa cctatattaa
     7681 cctaatttca gaattaaaaa taaactatta taattttttt ataaacataa gtcttgcctg
     7741 ca
//
LOCUS       DAMJVI010000142         7701 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.147, whole genome shotgun sequence.
ACCESSION   DAMJVI010000142 DAMJVI010000000
VERSION     DAMJVI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7701
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.147"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            105..2333
                     /locus_tag="PC545_003346"
     CDS             105..2333
                     /locus_tag="PC545_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDF2458067.1"
                     /translation="MRHLKKTNSLKEILNWNNIKNLRPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITDDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVIDNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSISALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAEAKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIDTLSKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIVLLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRVIKPFNDKFGDSNLT
                     GH"
     gene            complement(2571..2870)
                     /locus_tag="PC545_003347"
     CDS             complement(2571..2870)
                     /locus_tag="PC545_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDF2458068.1"
                     /translation="MYKNINKKQLKEMMKNEKDVLLLDVRTKDEFKEYKIENAINISL
                     QELINNIDEIYDYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMS"
     gene            3077..6118
                     /locus_tag="PC545_003348"
     CDS             3077..6118
                     /locus_tag="PC545_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HDF2458069.1"
                     /translation="MFKQRLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNAESYTKTKALAKEKIQTLLSKYGAVSAQYALIDNGKIEISGNGGVYSKQD
                     NKNLNKDNMYSIASISKMFTTTAVMKLVDDGKLNLDTPVVKYIPEFKMADDRYKEITP
                     RMLLNHSSGLMGSSFKNTILLADNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTPENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGSSQLNEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSSLKENSGLYASSNMIKVDVNDNG
                     TLTVSSPYIENGPEDKYVYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRSGKGYYLVDEKYTSQSYMFGSVKASFSLSD
                     ETPGYIVNTKIMDENNSNAFIEIPGVIGRDLSDIKLHKENGTEYLSFGTLTYVSEDSI
                     TNLPAEKSFTCELESNGYAKWYKIGDDIANKKIEVNLPQNSAFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINEKSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVM
                     LVGNSLDYNQKELFKTMRFKSVTQIGGNGNENSFKQIKEIA"
     gene            complement(6279..7553)
                     /locus_tag="PC545_003349"
     CDS             complement(6279..7553)
                     /locus_tag="PC545_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDF2458070.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKIECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHENCVKCGKCITACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
BASE COUNT         2847 a         1074 c         1102 g         2678 t
ORIGIN      
        1 ttaaaattaa gacaaaattt taactttact atttgtgtaa aaagtaatat aatttaaaat
       61 tgattataaa tctaactttt ttctttatca aaatatttta ggaggtgaga catttgaaaa
      121 aaactaatag tttaaaagaa attttaaact ggaataatat caagaattta agaccttcaa
      181 agggtttaaa aaatatatta gaaattttta aaagtgacct aaaagatata cgtggcaatt
      241 atgccgtact tgtaatagtt ttaggtcttg cttttttacc atctctgtat gcttggttca
      301 atataaaggc atcttgggac ccttatggaa gcactgctaa tatgcttgta gcagtaacaa
      361 atgaagataa aggaaatgaa atagcaggac aaaagattaa tgtaggagac caattaattg
      421 aacaactcaa agataacaag agtttaggtt ggcagtttgt agatgaaaaa actgctcttg
      481 aaggtgttaa aaaaggtact tattatgcat cacttaagat tccaaaaaat tttacaaaag
      541 atttgacatc tttaattaca gatgatgtta aaaaaggcga aattatctat actgttaatg
      601 aaaaaataaa tgcagttgca ccaaagatta cagataaagg tgcttctaca atacaactac
      661 aggttaatca gactatagta aaaacagtta gtgaaatcgt tcttggtata tcaaacggag
      721 ttggtgcagg aattgaacac agtcttccaa aattgacaaa agttgaaaat tcccttattg
      781 accttcaaaa gagatttgac catataaata agactgttga tttagcttct gatgctactt
      841 caaaattatc tgatacagtt aaagatatta aatctgattt acctacaatt aaaaagactc
      901 tgaatgatac aaaactatta tcaagtgact taaaaaaatt cttagaggat acaaatgata
      961 atttagacga actttctcct cttattaagt ctgacttaaa tttaatggtt gacctttcat
     1021 cttctgcttc ttctcttaca ttaaatttaa ttgatgcagt aaatagtggc agtgaagatg
     1081 tacctaagtt gatagataat ttatcagaaa aactttctaa tttgcaatct ttaaatgata
     1141 ctttggtgga ttttttaact aaattaaacc aacttacatc aaataataga ttggatgatg
     1201 tcatagataa tcttgaagac tctagcaata aaatagattc atctatatca acattaaatg
     1261 atattaaaaa caaagttata tctggacaac aaccatctat aagtgctttg aataatgttt
     1321 taagtctatc aaatggtatt ggaagaatta atttaaatat tttaaataat tttgactcaa
     1381 aaatatcaaa acctataaat agtatatttg caaacagtat aaaagtagca aatgatataa
     1441 ttactgtttt agataaggca gaagcaaaac ttccaaaggt tgaagaaata cttactactt
     1501 ctctaaaact ttcaggaaat gcacaggaaa gtatatcatt gataagagaa agacttcctt
     1561 tagcaaaagg aatgttggat gatttgattg atactttaag caaaattagt aatggtgaag
     1621 atatgaaaaa attagtcagc ttgcttgaaa gtgatatatt gacaaagtct aatttcttaa
     1681 aagaacctgt tgaggtgaaa acagagaaat tatatccaat tgaaaattat ggttctgcaa
     1741 tgacaccatt ttatagtgtt ctttctacat gggttggtat attacttatt tcagcactat
     1801 tgtctacatc tgtacatgga aactataaac cttatgaaat ttactttgga agaggcctta
     1861 cttttttaag tatagctctt gtccaaggtt ttataatagg aactggagat atattattgt
     1921 taggagtaac agcaaaacat ccaattttat tgatagttct gcttatgttt actagtatgg
     1981 tatttaactt tatagtgtat tctttagttt ctgtatttgg aaatgtagga aaagcaattg
     2041 caatagtact attagtcgtt cagattggtt cttctggtgg tacattccct atacaagtta
     2101 cacctacatt cttccaaaga ataaacccta taatgccatt tacctatgca ataggtgcta
     2161 caagggaagc agttggtggt atatatgctc caaatctaac taaagatata ctagttctat
     2221 tactatttat gattctgtct atttgtttaa atgtacttct aaaaggacct ataaatagag
     2281 ttataaaacc atttaatgat aaattcggcg atagtaattt aactggtcat taaaaaatta
     2341 attctgtctt taaatatatt tttttaataa tattctaagt tcatattaaa aggtagtagc
     2401 taaaaagcta ctaccttttt ccaacttaaa aactaatatt tactacaaaa actgtcatag
     2461 aaaaaaacga aaaaattcgt tctttcctaa atttatctta taaatattta gatatattcc
     2521 tacttaataa gcagagctat acatagtatt taatgtataa cttacattat ttaactcata
     2581 taatcaatta ttcctgaatt taaattatat aaatgctcaa aaccttcttc agctaacaaa
     2641 ttacatgcag taacacttct atgaccactt ctgcaataaa ctactacttt cttatcttta
     2701 taatcgtaaa tttcatctat attattgatt aactcttgta aagatatatt tatagcattt
     2761 tctattttat attccttaaa ttcatcttta gttcttacat ctaataataa aacatccttt
     2821 tcatttttca tcatttcttt aagttgcttc ttatttatat tcttatacat atgtctaaaa
     2881 ttcctccatt ttatttatat attacctata ccctttattc tttatacaac aacaaatatt
     2941 atttttatat ttttctctgt tatataaaaa gctatactta taaaagcttt ttttattgat
     3001 aataatctgt tgattaaaat aattaagaat gttatgctaa ttataaatta ttaaaaaggg
     3061 aaagaggtaa tgtaatatgt ttaagcaaag gctctctaaa ttattatcta gtacattagt
     3121 attgagtatg ttatttactg ctgctccaaa tattactttt gctgataata caaaagataa
     3181 ttccgaaaaa tatcaaagtt ctgatataga attacatgat tatagtaaaa acgcggaatc
     3241 ttacacaaaa actaaagctc ttgctaaaga gaaaatccaa actttgctat caaagtatgg
     3301 tgctgtaagt gctcaatatg ctttgattga taatggcaaa attgagattt ctggtaatgg
     3361 tggagtttac agcaaacagg ataacaaaaa cttaaataag gataatatgt atagtatcgc
     3421 atctatcagt aaaatgttta ctacaacagc tgttatgaag ttggttgatg atggaaaatt
     3481 aaatttagat actcctgttg taaagtatat acctgaattt aaaatggctg atgatagata
     3541 caaggaaatc actccaagaa tgttattaaa tcactcatca gggttgatgg gaagtagttt
     3601 taaaaacact attttattag cagacaatga ttcttatggt catgataatt tcttaaaaga
     3661 attacaaaaa caaagattaa aagctaaacc aggagctttt tctgtttatt gtaatgatgg
     3721 atttacttta gctgaaattt tagtagaaag agtatctggt atgtcattta ctaattttct
     3781 tgataaatac ataaataatc cacttaactt acaaaataca aaaactccag aaaatagttt
     3841 tgatagttca aaactagcaa aagcatatgt accttattgg gaagatgcag ttccacaaga
     3901 taaccttaat gctataggtg caggtggttt atattcaagt gcagaaaatc tatgtacatt
     3961 tgcacaaaca tttatgaaaa attcaaatgg tattctttct cctgcttcag taaaagctat
     4021 ggaaaacaaa gaatatttaa atggtctatg gcctgaagga gaagactcta tacttggata
     4081 tggtttaggg tgggattgtg tcaacactta tccatttaat cagtataatt taaaagctct
     4141 tacaaaaggt ggagattctt tattattcca cagtaatctt atagttcttc cagatgaaaa
     4201 tatggcagtt gcagttcttt cttctggtgg aagtagtcaa cttaatgaaa taattggaca
     4261 agaaatctta ctatctgcat taaaagaaaa aggtaaaatt aaagaaataa aacctgataa
     4321 aacatttagt aagccacaac aagttaaaat gcctagctct ttaaaagaaa atagtggtct
     4381 ttatgcttct tcaaatatga taaaagtaga tgttaatgat aatggtactt taacagtatc
     4441 ttctccttat atagaaaatg gacctgaaga taaatacgta tacataggtc aagatagatt
     4501 tgtatctgaa aaaggtaata gttgtttaaa atttgtaaaa gaaaaaaata atatcacata
     4561 tttaaatatg agctcttatg atgatgtccc tggattaggt cagacagcaa gtttatacta
     4621 tgtagctcaa aaagtagatg acaacaatat atcaaatagt gtcaaagaag tgtggaaaaa
     4681 aagaagtggc aaaggttact atctagtgga tgaaaaatat acttctcaaa gctatatgtt
     4741 tggctctgta aaagcaagtt ttagtctttc tgatgaaact ccaggttata tagttaacac
     4801 taaaataatg gatgagaata actcaaatgc ttttatagaa atacctggtg taataggtcg
     4861 tgatttatcc gacattaaac ttcataaaga aaatggtaca gagtatttat cttttggaac
     4921 actaacgtat gttagtgaag attctataac taatttgcca gcagaaaaat cttttacttg
     4981 tgaactagaa tccaatggtt atgcaaaatg gtataagatt ggtgacgata tcgcaaataa
     5041 aaaaatagaa gttaatcttc ctcaaaattc agcttttgct gtttatgatg ataaaggtgt
     5101 tcctgtaaac tattctttag ttacaaagaa caatagagtt agattgccaa aaggaggcgt
     5161 aattgttttc ttaggaagtc caaatgctag atttgaggtt acatatcaag atgaagtaaa
     5221 tgcaagtgca cttacaggaa ctgatagata tgaaacttct attaagataa gtcaagcagg
     5281 ttgggaaaat gctgaaaatg cagttttaat aaacgattca gcaattgcag atgctttggc
     5341 tgcaacacca tttgcataca agaaaaacgc tcctatatta ttgactggaa gctctcaaat
     5401 aaatgaaaaa actttggctg aattaaagag attaaaagtt aaaaatgtat atgtagttgg
     5461 tggagaagct tctataaatg aaaaatcttt agatactata aaatcaaaca atatctctgt
     5521 atctagaatt tcaggaagtg atagatatca aacttctatg aacattgcta aagaattgaa
     5581 caatattagc aatatatcaa agatttcagt agttaatggc gaaaaaggat tagccgatgc
     5641 agtaagcatt ggagctgtat cagctcaaaa tgatatgcct ataatactta ctaatgaaaa
     5701 tagtaatata actgaaataa ataacttgtt taaaaacaaa aagattgata aatcttatgt
     5761 aatcggtgga gaatatacag tttctaaaaa tatagaaagt aaacttcaaa atccacagag
     5821 aatatcaggt agcactagaa atgaaactaa tgcaaaagta ataaaagaat tttacaagga
     5881 ttctaagata gacaatttat atgttgctaa aaatggaatg aataaacaag atgatttaat
     5941 agacggatta tctgttggag ttttggctgg aaaaactaaa tctcctgtaa tgctagtagg
     6001 aaactcacta gattataatc aaaaagaact atttaaaact atgagattta aatctgtaac
     6061 acaaattggt ggaaatggaa atgaaaactc atttaagcaa ataaaagaaa tagcttaaca
     6121 taactaaaaa aggctgtatt caagtaatat taattgaata tagccttttt ttatttaagt
     6181 tataagtttt atatgtgtaa aaattaaatt taaatattaa atgataagtt tattaagata
     6241 tagataagtc taaaactttg ccttttttaa gttagtttct atttatcatt catacttaac
     6301 aatttatcta aatatactga ctttaattgt ttactggcta aaatcttttt agctggttca
     6361 agctttaata tagctgacag tatagctccc atagctctat gactagccag agcattatta
     6421 tcaaaaatta aattttgtaa ctgccctttt tcaattgcca taagaactgc tctatgtact
     6481 gaatttgctg gagttattat tttttcatct ctctttaata acataatgct atttttatgg
     6541 caatttctaa cacaaactcc acaacctaga catctatcct catcaatttt tatatattct
     6601 ttaccatctt ccttaacctt gcttatcgca tctattggac atgcagttat acatttacca
     6661 cactttacac aattttcatg gtttatattt ggtataaaac ttgttgtctg tactggatga
     6721 agatttccaa atcttttagc tgctaccatt gcttcacagc aacatccaca gcaattacat
     6781 ataaacgtaa cccctttcct aacattttct ccacattgaa ctaagttatg ttcatatgct
     6841 tgatgaagta gttctttaca ttctatttta tccacacgtc ttgcaaattt attatttatt
     6901 aaagaatttg ctacattgtc aaacgtcata catatatcca taggagcatc acaagctttt
     6961 ccaacatgtt gcattctatg tctacagtaa cacatactta tacctatatg agttgattca
     7021 tcaattatat gagtagccct ttcataatct aaaatactaa cctcattatc attagttaaa
     7081 acttcctctt gaacgtatac tcttccaagc ttcgtttctg ttgaataaaa taaatctttg
     7141 ataaaatctt cttctacatt catatattga tagtaaagct ctgccaaaag tttttggtct
     7201 atatcgtgtc tcgttctcat catggcaaac tcaaaaaaac cagccattgg aggaggcatt
     7261 atatatttct tccctttatt atcttctata tctagtatca gagccttact agcaagttta
     7321 tccaatactc tatatgcttc actttcactt acactccaaa ttttagctgc tgtttttact
     7381 ctgaaaggct ttattggaag ctgagcaact aacttcgctt cttgttctgt atatagtaca
     7441 tttagtattt tgtataatgt atcagatggt ggagcacctt gtggaaactt gtttattctt
     7501 tcctctaaac ttttatacat attcttcgca gttatatgag aaatagtaat caccttcttt
     7561 ttcttttgtt ttccaataaa atcaaatatt ctttcttatt atatattctc ttactttata
     7621 taataccaat tctttctaaa tagttaaata cctctagtaa tttattatta gatttcttat
     7681 ataaataaaa aatcatttaa g
//
LOCUS       DAMJVI010000143         7686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.148, whole genome shotgun sequence.
ACCESSION   DAMJVI010000143 DAMJVI010000000
VERSION     DAMJVI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.148"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            244..1545
                     /locus_tag="PC545_003350"
     CDS             244..1545
                     /locus_tag="PC545_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2458071.1"
                     /translation="MGKSKDVEIEKSILGTILLDNKLSYKLDELNENMFMNDICLEIF
                     KIMKELKKENIVIDVATVKSKIDRKSLAIKTSDVTNLITWGQNFGLDGHIKILKENLA
                     RRSINQNCQNLLHSLNLGENIDTCIYKFESNIKEILDKDTYENDDVNSIAGKVLDFLE
                     NKKDIGFKFGIKLLDTTIGGLFKGELTTIAAKSGVGKTALALQIMLNSFKQGKKTLFI
                     SREMTSEQVFMRNICRVTGVSTRNMKSKEIDENDWKLIVNAIGDLSENNLIYINDKID
                     TISAIRKRIRQVKPDLLIVDYVQLLTSQKSMDKREREVATFSRELKNMTLDFNIPVIQ
                     LSQLNDEMKDSRPYGDRPMRDSKAIYHDSNNVVYIHQLKGSDYEEAVRDIGESEEAVR
                     ASEYRGIKMVDLIVAKCRDGQTRHKHFCYFGDKLHFQELNY"
     gene            1621..1896
                     /locus_tag="PC545_003351"
     CDS             1621..1896
                     /locus_tag="PC545_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2458072.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELMIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            3284..3514
                     /locus_tag="PC545_003352"
     CDS             3284..3514
                     /locus_tag="PC545_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2458073.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            3507..4088
                     /locus_tag="PC545_003353"
     CDS             3507..4088
                     /locus_tag="PC545_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2458074.1"
                     /translation="MNNKKHWYDYLWIFSSIYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVIFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMILFKPRSWCVYCPMGTMTQMICKAKSKSI"
     gene            complement(4663..5340)
                     /locus_tag="PC545_003354"
     CDS             complement(4663..5340)
                     /locus_tag="PC545_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDF2458075.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTVRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            complement(5529..6893)
                     /locus_tag="PC545_003355"
     CDS             complement(5529..6893)
                     /locus_tag="PC545_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDF2458076.1"
                     /translation="MKESNKRIELLGSAPISKALLSMGLPTMIGMMITALYNLVDAYF
                     VGGLGTSQMGAISVAFPLGQLVVGLGLLFGNGAASYIARLLGHGDNEKAGKVVSTSIY
                     SSIIVGTLVIIVIMIFLNPILKLLGATKSIMPYAISYASIYVVSSIFNIFNVTMTNIV
                     TSEGAAKTTMCAMLLGALLNMILDPIFIYTFGLGVRGASIATAISQIASTLVYVGYIL
                     SKKSIFSFRINECCFSKEIFSEILKIGIPTLIFQLLTSLAITLTNMEATKYGDSVIAG
                     MGAVTRITSLGSLVVFGFIKGFQPIAGYNFGSKNYDRLHIAIKTSILWTTIFCVVLGL
                     ILILFPKSIISQFTSGDILLIENGSKALRANGFSFMLFGFYTVYSTLFLALGKAKEGG
                     FLGMCRQGVCFIPIILIIPVFYGLNGILYAQPIADLMAAIIAGFMSIRLHKELNLLKI
                     NGMH"
     gene            7021..7542
                     /locus_tag="PC545_003356"
     CDS             7021..7542
                     /locus_tag="PC545_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDF2458077.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKAVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPDTAKAVLEQQLVLAQAL
                     ETWINSLKDNMGN"
BASE COUNT         2996 a          869 c         1326 g         2495 t
ORIGIN      
        1 attaataatt ggtagtaaag tatatcaaat aagatattgc tattatgtag taaattatat
       61 aaaacaatgt atcagaataa atagtatatt aataatttgt agtatatcat gacgaataat
      121 acagtcataa caaatagtga gtcataacaa ataatgtatt tataattgat aatgtattta
      181 taattgataa tgtatttaaa aatcaaaata taaaaactta taaggaaaga gagattaatt
      241 tatatgggta aatcaaaaga tgttgaaata gagaagtcta ttttaggcac aattctttta
      301 gacaataaat taagctataa gctagatgaa ttaaatgaaa acatgtttat gaatgatata
      361 tgtcttgaga tatttaaaat tatgaaagaa cttaaaaaag aaaacatagt gattgatgtg
      421 gccactgtga aatcaaagat agatagaaaa tctctagcaa taaaaacaag tgatgtgact
      481 aatctaatta cttggggtca gaattttggt cttgatggtc atattaaaat tttaaaagaa
      541 aatttagcta gaagaagcat aaatcaaaat tgccaaaacc ttttacacag cctaaacctt
      601 ggagaaaata tcgatacttg tatttacaaa tttgaaagta atataaagga gattttggac
      661 aaggatactt atgaaaatga tgatgtaaat agtatagcag ggaaggtgct tgatttttta
      721 gaaaacaaaa aagatattgg atttaaattt ggtataaaat tacttgatac cactatagga
      781 ggtcttttta aaggagagct aaccacaata gcagctaaat cgggggttgg gaagactgct
      841 ttggcacttc aaataatgtt aaattcattt aagcaaggta aaaaaacttt atttataagt
      901 agagaaatga ccagtgagca agtatttatg agaaatatat gcagagtaac tggtgtaagt
      961 acaaggaata tgaaatctaa agaaatagat gaaaatgatt ggaaacttat tgtaaatgct
     1021 ataggtgatt taagcgaaaa taacttaatt tatatcaatg ataagataga tacaattagt
     1081 gcaattagaa agaggataag gcaggtaaag ccagatttat taatagtgga ttatgtgcaa
     1141 ctccttacat ctcagaaaag catggataag agagaaaggg aagtagctac tttttcgaga
     1201 gaacttaaaa atatgacttt agattttaat atacctgtaa tccaattaag tcagttaaat
     1261 gatgaaatga aagattcaag accttatgga gatagaccta tgagagatag caaagccatc
     1321 taccacgata gcaacaatgt ggtttatata catcaattaa aggggagtga ttatgaagaa
     1381 gcagtgagag atattggaga aagtgaggag gcagttagag catcagaata caggggaatt
     1441 aagatggttg atttaatagt tgcgaagtgt agagatggac agactaggca taagcatttt
     1501 tgttactttg gggataagtt acattttcaa gaactaaatt attaggtcaa taaagtagta
     1561 ataacaagct tattaaaagt cggggaatag caaatagatt ataaaatatg aggtgaaaat
     1621 atgttggaat taacaaaaat tatagaatta cttgaagaac acaagataga agtaagtatt
     1681 ccaaaggagt tacataagta tagtttattg gaataccttg aattgtgtca acaaagtggg
     1741 ggaacatatt tttatattaa aacactggaa ggggctttaa aagaggctag aaatgagctt
     1801 atgattaaat tgtttaatgg gaaaaattat atggaattag cgaggatgtt tgaaatgagt
     1861 gttattaatg tcaggcatat tgttagaaaa aaatagatat ttttggagaa tacaagtgtg
     1921 atatacttct gaaatagaag ttattatagt tgaaataaaa ttgtggttaa taaattaaag
     1981 ttgtgtataa gtttaagatt atagaggagt ataagtgtga ttaacagcat gattttgtga
     2041 ataaaattga ggatatatat gagttaaatg tgtgattaat aaggtaaggt tgtgtataag
     2101 attatgagta tttaaaagga ggggctataa ttaatagtgt agagttgtgt gtttgcttaa
     2161 gattatataa taatttaata gattaaacta tactaataag gtaaaatcgt gtgtttagtt
     2221 aaagcttatt ataaaatctt gatatgtgat taatgatgca atatgtgaat aaagttaaga
     2281 ttataataga tttaacctgt ggctaataga ataaaattgt gaataaagtt aagattataa
     2341 taggtttaat ctgtgactaa tagagtaaga gtgtaaatag agataaactt caatatgagc
     2401 ttaacctatg attaatagag taagagtgta aatagagata aacttcaata tgagcttaac
     2461 ctatgattaa tagagtaaga gtgtaaatag agataaactt caatatgggt ttaacttgtg
     2521 attaatagag tagggtgtaa ctaaagataa acttcaatat gagtttaact tgtgattaat
     2581 agagtaaggt gcaactaaag ataaacttca atatgagctt aacctatgat taatagagta
     2641 agagtgtaac taaagataaa cttcaatatg ggtttaactt gtggttaata gagtaagagt
     2701 gtaaatagag ataaacttca atatgggctt aacttgtgat taatagagta agagtgtaac
     2761 taaagataaa cttcaatatg agcttaacct atgattaata gagtaagaat gtaaatagag
     2821 ataaacttca atatgagttt aacttgtggc taatagagta agagtgtaaa tagagataaa
     2881 cttcaatatg ggtttaacct gtggttaata gagtagggtg taactaaaga taaacttcaa
     2941 tatgagttta acttgtggct aatagaataa gattgtgaat aaagttatag ttttagatgg
     3001 gtattaacct gttactaata gaatatgatt aggtgtaaaa ttatagagct gtataagaaa
     3061 atattataga tatattaaat attagataat aaatatttat atagatacac aaaagaaaag
     3121 gcgatatgaa gctaaactta atgacttcat atcgtttttt atttgaaact cctttgtatt
     3181 tagagtgtga tttttaatat tgaggtttta tgtgattaag tcacagataa aaagtaagta
     3241 tcttgatata attcttatag attaataata tagggaggtt attatgtcta ataaaaaaaa
     3301 gaagtatgca aatattttag caaatgagtg cgttgcttgt ggtagctgta taaaagcatg
     3361 tccaagaagt gctatctcag ttccatgtgg cattagtgca aaaattaata gagatttatg
     3421 tgttggatgt gggatttgtg aaaagatatg tccagcatct gtaattgaga ttataacaat
     3481 attaaaagag gaagaaggta agtgtcatga ataataaaaa acattggtat gactatttat
     3541 ggattttttc ttcaatatat ttgatattgg gatttgtaaa tattattttc gcttggattg
     3601 ggctaatttg tttctttgtt ccactagcta tttctattgt taagggaaat aaagcttact
     3661 gtaataagta ttgtggaaga gggcaactgt ttaatttgct tggaaataaa ttgaagctgt
     3721 caagaaatag agatattcct aaatttatta gaagtaagtg gtttagatat gggtttttga
     3781 cattttttat ggtaatattt gccaatatgc ttttttcaac ttatttagtg tttgaaggaa
     3841 gtagaaattt taaagaagta gtgactttgt tgtggactat aaaaatgcct tggcaatggg
     3901 cgtatcatgg aactttagtt tcaccttggg tggtacagtt tgcatttggt ttttatagtg
     3961 ttatgcttac atcaactgtt cttggtttga ttactatgat attgtttaaa cctcgttcat
     4021 ggtgtgttta ttgccctatg ggtactatga ctcagatgat atgtaaggca aagagtaagt
     4081 ctatatagaa aggctttatg tgatatttac agttttgtta gagatgagta gtaggtttga
     4141 atatatatga caaatatatg agtatagggg catagattta gtttgtgtaa ggtaaactga
     4201 tttatgcttc ttttttatgt cttagattat tggctaagtg gaaacaatga atttagatat
     4261 taagaattat tcagttattt gaagaaaata gaataataat taacattata tgaatggata
     4321 ttcattacat ataagccagt taatagcaag atttatttaa tatgaaatag gtcaagtatt
     4381 taaactaaat aaggtcatga caattaatat aagtatagat ataaaaagat tagaagttat
     4441 aacttataaa aggtattcta tataaatagt atgtggaaaa tagtcaaatt aacttattag
     4501 aaacaaaaaa tatacataaa aaaataaatg taaatatcaa ataagatact atataaaaat
     4561 agatataagt atctttttta ttattgaaat tatgaaaata tatttgattt cacacaaaaa
     4621 agagtgcctc atgaactaaa aagttcattt tgagacacac ttttacagtg tcaaagaacg
     4681 aagtttatcc ttatccaata cttttatacc accacgagat aaagacacaa taccttctct
     4741 agcaaaatat ttcaacattc tcgatacaac ttctctggca ctacccatat acttagcaat
     4801 ttgctcatga gtcatattta tagtgctact gccattttta gctatctcat caattaaaaa
     4861 agatgccaaa cgcttatcaa aactcataaa aagaatttgt tgcatagccc acataacatc
     4921 agaaaaacgt tcggtagcta atttatatga aaataactct gcatgaatat tttctgtaga
     4981 taattttgca aaaacagagg aactaatctg tataatatcg cagtccgttt cagcatctac
     5041 atagacatca aaagttattg tttttagaat acaggaagct gataaaatac atacatctcc
     5101 atcatcaagt cggaacagtg taacctctcg accttcatct gaaagtatgt atgtacgtac
     5161 tgttccagac tttataatca aaataccaat acagtcacta tcaccacaat gtactggatt
     5221 cccttttttg taatgcgagg tatttgctga agaaattaat aattcttttt gtaagtcagt
     5281 aagtttatcc caaaaaggca agctatgtgt aagtatatcg atttcatttt gtgaaatcat
     5341 acaatatctc ctttcttaaa ttgttataat cccatgatac aagtgaatag ataaattgac
     5401 aagtagtttg ttaataggat tatttagtca gatagtaata gtatgattta tgtagaaaaa
     5461 atatgataat atatattctt gctattaatg agaaggtaat atgttttgat gatagtaggt
     5521 ttgtataact aatgcatacc gtttattttt aaaagattaa gttccttatg taaccttata
     5581 gacataaaac cagcaattat agcagccatc aaatcagcta ttggttgtgc atataagata
     5641 ccatttaacc catagaatac aggaataatc aaaattatag ggataaaaca gacaccttgt
     5701 ctacacatac ctaaaaatcc accttccttt gcttttccta gtgcgaggaa taatgtagaa
     5761 tacactgtat agaatccaaa tagcataaat gaaaacccat ttgccctaag agctttacta
     5821 ccattttcta ttaatagaat gtcaccactt gtaaattgtg aaattataga ttttgggaag
     5881 agtatcaata taagaccaag cactacacaa aatattgtag tccataatat ggaagttttt
     5941 attgctatat gtaatctatc gtagttcttt gaaccaaaat tataaccagc aattggttgg
     6001 aaacctttta taaaaccaaa tacaacaaga ctacctaaag acgttattcg tgttacagca
     6061 cccattccag ctatcacaga atcaccatac tttgtagcct ccatattagt taatgtaatg
     6121 gcaagactag tcaataattg aaatattaag gtaggtatgc caatttttag gatttctgaa
     6181 aaaatttctt ttgaaaaaca gcattcgtta attctaaaac taaatatact tttcttgctt
     6241 aaaatatatc ctacataaac aagtgttgaa gcaatctgtg agatagctgt tgcaatagaa
     6301 gcacccctta cccccagacc aaaagtataa atgaatattg ggtctaaaat catattaagc
     6361 aaagcaccta ataacatagc acacatagtt gttttagcag ctccttcact tgtgacaata
     6421 ttagtcatag tgacattgaa tatattaaaa atagatgaca cgacataaat gctagcataa
     6481 gatattgcat aaggcataat actttttgta gctcctaaca attttaaaat tggattgaga
     6541 aaaatcataa tcacaataat aactaatgta cccactataa tactactgta aatagatgta
     6601 cttaccactt ttcctgcttt ttcattatca ccatggccta aaagtctggc aatatatgaa
     6661 gcagctccat ttccaaacaa taatcctaat cccacaacaa gctgacctaa tggaaatgca
     6721 acggatatag ctcccatttg acttgttcca agtccaccca caaagtatgc atcaaccaaa
     6781 ttgtataatg cagttatcat catacctatc atggtaggta atcccataga taacaatgct
     6841 tttgagattg gggcagaccc taataattca attcttttgt tactttcttt catacttaac
     6901 actcctcttc tattgacatt aagtctaaat tataatacta taaattatag taagtatgga
     6961 aaatagtact ataatttata gtatttgtca atagtaagaa aactaaagga gatgattttt
     7021 atggctacaa tagatttaat tgtattggga atagtaaagc aacaacccaa gagtgcttat
     7081 gatattcaaa aggcagtgga atacaggaat atatcacgtt gggttaaaat aagtacacca
     7141 tcaatttata aaaaagtaat tcaattagaa caaaagggat atattgaaag taaaactgtg
     7201 aagaatggaa aaatgccaga aaaagcagtt tattcattaa ctgaattagg tgagacacat
     7261 tttttagatt taatgtttga tacatctagc aaaccaataa atattctttt agattttaat
     7321 gctgttatag taaatttaaa tagccttaca ccagaaaaac agaaattatg tctcaataat
     7381 attgaagaga atgttcaaga gttaaagaaa actattgagg aaaatataag tataaagaca
     7441 gaagtaccag acacagcaaa agcagtgttg gagcaacagt tggtgctagc acaagcattg
     7501 gaaacctgga taaattcttt aaaagataat atgggaaatt agaattagag aagctaactt
     7561 aataatcatt aaacaaaaac cagcatattt aataatgaga tattagaaaa aataaaacaa
     7621 atgatataaa ctgaaaagta tatttaaaat aaagctcact taattgaggg agaatgttaa
     7681 actttt
//
LOCUS       DAMJVI010000144         7591 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.149, whole genome shotgun sequence.
ACCESSION   DAMJVI010000144 DAMJVI010000000
VERSION     DAMJVI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.149"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            108..449
                     /locus_tag="PC545_003357"
     CDS             108..449
                     /locus_tag="PC545_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HDF2458078.1"
                     /translation="MKEENFINVEYEDKRFSINSKEVLYIEKQEYEVKIYTESGNYEV
                     GENAPCIRKLLKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            600..1013
                     /locus_tag="PC545_003358"
     CDS             600..1013
                     /locus_tag="PC545_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HDF2458079.1"
                     /translation="MLELKNNLGISERKLKYPCIYKHFKGKYYATMGLSKAIDHIENI
                     CEIYGKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFARPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1215..1856
                     /locus_tag="PC545_003359"
     CDS             1215..1856
                     /locus_tag="PC545_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDF2458080.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILGDKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIVKMNDTSHIKNHVKINNSALE"
     gene            1880..3133
                     /locus_tag="PC545_003360"
     CDS             1880..3133
                     /locus_tag="PC545_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDF2458081.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNREIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEVLNKDLLNHNVKIMYNKKVIDIITEEIALKDDSNKDKSKYLIKGI
                     ILDNGDKILGDKVIISTGGVSYSKTGSDGSMYKILKKHGHTLNKLYPALVPLTIEEKW
                     IKDLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSTDEISSIIRAFPNKNVLNNLKQILPQNFLKEIFSLLSLVDKKASDLSK
                     ADEIRIIEYIKNMKLTCNGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3179..3829
                     /locus_tag="PC545_003361"
     CDS             3179..3829
                     /locus_tag="PC545_003361"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDF2458082.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGIDVNNEKEVIQIAENSDIDFKDNNIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVEVQRKIGMKNSVILDGRDIGSYVFPDADYKFFLVATPEERGNRRYKELCNKG
                     YNTTLEEVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVVSKINL"
     gene            3880..4476
                     /locus_tag="PC545_003362"
     CDS             3880..4476
                     /locus_tag="PC545_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2458083.1"
                     /translation="MNFYRFVINIFKGFSKIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAIENREIAAIAKKELFKVKPLGFILKKLHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLSNDDHERISQEILEVIKQNYFKYSK"
     gene            4489..5328
                     /locus_tag="PC545_003363"
     CDS             4489..5328
                     /locus_tag="PC545_003363"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDF2458084.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKNDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKDKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKRYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKKVDCMIVIGGK
                     HSSNTQKLVKVCEDLVPTFAIETKDELDVNTLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5573..6727
                     /locus_tag="PC545_003364"
     CDS             5573..6727
                     /locus_tag="PC545_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HDF2458085.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDISKNNPKENKEQHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIIVIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMIITTIPAILVGMIVYAFLG"
     gene            7033..7209
                     /locus_tag="PC545_003365"
                     /pseudo
     CDS             7033..7209
                     /locus_tag="PC545_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT         3142 a          734 c         1339 g         2376 t
ORIGIN      
        1 taattattta aatatcttaa gtaaatcagt cttacatata tttccgatag attatattga
       61 taaaaaacac taaaattaaa ttaataaaag tacttggagg gatggaaatg aaagaggaaa
      121 attttattaa tgttgaatac gaggataaac gtttttcaat taatagtaaa gaagttttgt
      181 acatagaaaa gcaagagtat gaagtaaaga tttatactga aagtggtaat tatgaagttg
      241 gagaaaatgc tccttgtata agaaaactat tgaaagaaga aaattttttc aaatgcagta
      301 atagttatat tgtaaatcta gacaagtgtg aattttatct cagcaataat tgtatttttg
      361 tgaataataa ttttataaga attttaggtt cagaaagaaa aaagaaatta aaaaagaaat
      421 tagaaaaatt atatgattat gaagattaat attgggaggc tgtattttat acagcctctt
      481 tatttattga ttgtattatt aataaatttt aaattatgat aaaatataaa acaagtaact
      541 tatttcagaa taaaatactt tttaaaagaa agggacgaga tatgggagat tataataaaa
      601 tgcttgaatt aaagaataac ttaggtatca gtgaaagaaa gttaaaatat ccttgtatct
      661 ataaacattt caaagggaaa tattatgcta caatgggtct tagcaaggct attgatcata
      721 tagaaaacat atgtgaaata tatggtaaag aaaatttaat ccaaaacaga aataaatata
      781 aattagttat tagacatact gaaagagaag aagatatcta tgtttatagg gatttagatg
      841 gaaactttta tcataaaaaa gaagaagata ctaatgattt agttttatac aagaccttat
      901 atgatgatac aggaatattt gcaagacctt tggatatgtt tttagaaaaa gtagatacag
      961 ataagtatgt caattcaatt caagaataca gatttgaaga agtatataaa taatataaga
     1021 gaaatatgtt tataaattta gaaaaattct ttaacagtac aataatttgc agaaatacaa
     1081 atgagattta ttgtaaataa tatgttagat tagattataa aatagaaaca aagtaaagta
     1141 ttatatgata taattaagtt ttgagaagtg ataatttata tttaataaaa aataggcata
     1201 tattaggagg acatatggga aatacttttt atatagtaag acatggacaa acggattgga
     1261 atatattggg aaaaacacaa ggtcatggta attctgacct tacgcctcaa ggtatagaac
     1321 aggcaaaaga actttctgaa gatattggca agtattcaat tgattatatt tttagtagcg
     1381 accttgggag agcaatgcaa acagctcaaa ttttaggaga taagctcaat attgaagttc
     1441 aaaaaactga ggcacttaga gaaatgggat tcggagtttg ggaaggcctt ttaataaaag
     1501 aaattcaaaa agattattca gatatatatg caacatggag aaatgaacca cacttagtaa
     1561 atataccaga aggcgaaact cttaagatta taaaggaaag agtagatgct tttataaagg
     1621 aactaaatga aaaatatgat aataaaaata taattcttgt tacacattca attacattga
     1681 gggttatgtt gttgtcattt ttagaatcag gtatggaaaa tatatataga attaagcaag
     1741 ataatactgc tttaaatata gtagaattta aagattatgg gcctgttata gtaaaaatga
     1801 atgatacaag tcatataaaa aatcatgtaa agataaataa ttctgcactg gagtaggaat
     1861 caattataag gagaaattta tgagtaaagt tatagtagtt ggtggaggag cttctggaat
     1921 gatggcagct ttgtcagctt caaaaaacaa taatgaagta atattagttg agagaaatgg
     1981 agaattagga agaaaactta gggcaactgg tggcggtaga tgcaatttta ccaataatag
     2041 agagatagaa gatttttttg ataaagttgt cagtaataaa aaatttctct atagtagttt
     2101 ctatacattt acaaataaag accttatttc gtattttgaa agtagaaatt tggaatataa
     2161 gattgaagaa gaaaatgacc acaaagtata cactaaaaat gataaatcta tagaagtaat
     2221 agaagtatta aataaagatt tactcaatca caatgtaaag ataatgtata ataagaaagt
     2281 tatagatatt ataactgaag aaattgctct aaaagatgat tctaataagg acaagtcaaa
     2341 gtatctaata aaagggatta ttttagataa tggagataaa atactaggag acaaggttat
     2401 tatatcaact ggaggagtga gttattcaaa gacgggttca gatggttcaa tgtataagat
     2461 tcttaaaaag catggacata cattaaacaa actatatcct gctttagttc cacttacaat
     2521 agaggaaaaa tggattaaag atttacaagg aatatccatg aagaatgtgg aaatatcttg
     2581 taagattaag aaaaggaaaa tatcaaaatc tggagacatg ctatttgccc attttgggat
     2641 aacaggacct tgtgttctta taatgtcttc ttatattaac aagattattg aaaaggaaaa
     2701 ggtagaatta aatatagatt tcttaccaaa tttaagtact gatgaaatct ctagtataat
     2761 cagagcattt ccaaataaaa atgttctaaa taatcttaag caaatactac ctcaaaattt
     2821 cttaaaagag attttttctc tattatcttt agttgataaa aaggcaagtg atttgtctaa
     2881 ggcagatgaa attagaatta ttgagtatat aaaaaatatg aagctaactt gtaatgggac
     2941 tacaggtata aacactggta tggttacttc tggtggtatt tcagttaaag aaattaattc
     3001 ttctactatg gaatctaaat tagttaagaa cttatttttt acaggtgaag taattgatat
     3061 agatgcagaa actggtggat ataatttaca aatagcattt tcaactggat atcttgcggg
     3121 tataagtgta taaatataat tttttaataa ttaaatatga ataaatagag gtgtatatat
     3181 gggtaattta gttatagctg tagatggtcc agcaggagct ggaaaaagta caatagccaa
     3241 gatagtagca aaaaagctaa atataaatta tatagataca ggtgctatgt acagagcagt
     3301 tacttataag tgtttaaaaa gtggtattga tgtcaataat gaaaaagaag ttattcaaat
     3361 agctgaaaat tcggatatag attttaaaga taacaatata tatttggaca aagaagttat
     3421 aaatgaagaa ataagaacaa tagaagtaag caataatgtt tctaatgtag caaaaataaa
     3481 agaagtaaga cagttgatgg ttgaagttca acgaaaaata ggaatgaaaa attcagtaat
     3541 actagatggt agagatatag gttcatatgt tttcccagat gcagattaca aattcttttt
     3601 agtagctact ccagaagaaa gaggaaatag acgctataaa gaactttgta ataaaggata
     3661 taatactact ttagaagaag taattgagga tataataaga cgagatgaaa ttgattcaaa
     3721 tagagaattt gcaccattgg taaaagctaa tgatgcacta gaaatagata ctacaggaaa
     3781 aactatagaa gaagttgtag aagaagttgt atctaaaata aatctttagt tttttatgca
     3841 tcataatgaa aataaatgta gtgttgaagg gagataaacg tgaattttta tagatttgta
     3901 ataaatattt ttaagggttt ttctaaaata ttttttaaat atgaagttat aggtgcagag
     3961 aatattccag atagaggaaa tatagtaata gcatcaaatc ataaatcgaa tcttgaccct
     4021 atattcttgg ctgctgccat agaaaataga gaaatagctg caattgcaaa gaaagaattg
     4081 tttaaagtaa aaccattggg atttatatta aaaaaattgc atgttatgcc tataaataga
     4141 gaaaaaccag atgtatctac aataaaaact attttaaggt cagttagaga cggttatgta
     4201 ttaggaatat ttccagaggg tactagaata aaaggagatt cttttggtaa ggctaaggca
     4261 ggtctttctg tatttacaat aaaaagtaaa tctaaagttg ttccagtatc aataatatct
     4321 aaatataaac tttttagtaa agtaattgta tatattggag aaccaatatc atttgaagaa
     4381 cattttaagg aaaaattgag taatgatgac catgaaagga tatcacaaga gattttagaa
     4441 gtgataaaac aaaattactt taaatattca aaataagggg gtaaagttat gaacataaaa
     4501 atagcaaaaa atgctggctt ttgttttgga gtaaaaagag caatgaaaat ggcatgggat
     4561 gaagttgaaa aaaatgattc tggcatatat gctttaggtc ctcttattca taacaagcaa
     4621 gcagtagcaa aatatgaaga aaaaggtctt aagactgtga atgaaattga tacaatacca
     4681 aatcatgaaa atatgataat aaggtctcat ggagttcctg aaaatatata taaggaagcc
     4741 aaagataaaa aattaaaaat tgtggatact acatgtccat ttgtaaagaa aatacataca
     4801 gtagtaagtg aatatcataa taaaggctat gaaataatag ttattggaga tatgaagcat
     4861 ccagaagtta taggtataaa tggatggtgt gaaaattcag ctataataat aaagacctta
     4921 gaacaaatgg aaaatatgga atttgataac tccaaaagat attgtcttgt tgcacagact
     4981 acaataaatc cagagttgta tataagtata gttaataaac tatcagataa attagaagaa
     5041 atagtattta atgatacaat ttgttcagct acaaaaacaa gacaagaatc agctaaagag
     5101 ttagctaaaa aagttgattg tatgatagtt ataggaggta aacacagttc aaatactcaa
     5161 aagttagtaa aggtttgtga agacttagta ccaacttttg caatagagac taaagatgaa
     5221 ttggatgtaa atacgcttaa aaaatataaa aatttgggta ttacagcagg ggcatctaca
     5281 cctaattgga taatagaaga agttgtaaca ttccttgaaa atttataatt tgataggtaa
     5341 taaaaatagg tggacattgt tataatttat cataatatcc acctttgatt tcatattaca
     5401 tgtggtaaat agataaccta atatcaaaaa gtgtgtatct taagatgaaa aactaagagt
     5461 ataagatgcg cactttttaa tattagaaat tcaaaagaaa tattgatatt gattatcaat
     5521 agaaaataga atataatata attgttgcaa aattagtcta ggaggtaaat taatggaaat
     5581 gttgatatcc gcttctgaag aggcgttttt acatgtaggt tctatgattg gattttttat
     5641 tttgttattt ggatatatta attataaaac tagtggaaat tttactaata tcatatcaaa
     5701 aaacagaaaa tttcaaccct taataggtgc tttaattgga gctataccag gttgtggcgg
     5761 aagtttggca ataatgccat tgtatataaa tggaaaatta agctttggag caataattgc
     5821 aagtttaata gctagtatgg gagatgcagc atttgtactt atttcatcaa atattaaaat
     5881 gtattttttt gtcactatag ttagtacaat tacaggaata ataactggac aattagtgga
     5941 ttactttaaa ctagaagaaa aattagggtt aaaaaaccgt agaaaatcag ataaatatga
     6001 tataagtaaa aataacccta aagaaaataa agaacaacat aatcatgatg aaataatttt
     6061 agacactcta gctaaatctc atggaaatac aaatagactt gcatttataa taacacatgg
     6121 aaagggatat aaaatttata taggaattat agtaatcggt tttatattta tgtcacttgc
     6181 tcactctgga cttaatcttc ctataattga gaagttacat tcattggaag aagtaatagc
     6241 agtgattgga atattatttt caataattta tatgtggtgt tttaaaaagg tatttaaaaa
     6301 ttctaatcaa aaagaggaag aaaataaaaa gatttctttg cgagaaatgc ttatccattc
     6361 tgttggagaa atatcatttg taattacatg gatttttatt gcatatctta tttacgattt
     6421 gataatatta attttaggtg gagatgaata cttagttaaa ttagtattat caacaggtgt
     6481 aataagtgta tttataggtg caggtcttgg acttattcca ggttgtggga tacaaattgt
     6541 gcttatgtca ttttatttga agggaactat accacttggt gcagtgatag ccaactctat
     6601 ttctcaagat ggagatgcac ttttcccact tttagcaatg gataaaaaat cttctttatg
     6661 ggctatgatt attactacta taccagcaat acttgtaggt atgatagttt atgcattttt
     6721 aggttaaata taattatgaa taaagaagta ttaaaaaaat aataaagcac ataaatttat
     6781 ttataagaat agtgaagatt aaatataaaa ataaaaaatt caatatgaat taaattattg
     6841 cttaaatatt aatgagaaca tgggagataa tattattcct catgttctct ctatttaatt
     6901 aaaggaatta tggaacaaat ttcaagtgat actttctatc aaaactcata tataatatat
     6961 attaataaat taaactataa tacgtaatac agtcagtata ttgatataaa aaacttagga
     7021 ggagttgtaa aaatgggtaa tttaaaaaga cagtgtgatg tttcaagtgg aagagaaaaa
     7081 gctgacattg tactaaaaaa tgggacaatc ataaatgtat ttacagaaga gttaataaca
     7141 ggagatgtag caatagtagg agacactatc gttggaattg gcgattacaa gggcaatgta
     7201 gaaattgatt aaaaaatagc tacgcacact cgtgacttta gtcatgagtt agtagctaaa
     7261 atagtcagca tatagggaaa cctatatgta gagataggat agaacctatc caatactact
     7321 tgaattgctg gaaaccccta aagctaatca aactaaaaca taaggatgaa ataaacctaa
     7381 gtgtgaaagt tacgaaagta gaaaaaattg gttagatggt ataaggttaa accctaagta
     7441 ctttaaaatg ggcaatcagc aggtaaactc cgaataggag aaacttcaac gactattcct
     7501 cttgagggaa gtacactaca agctattggt agtggaagtg ggtagacctt aacggataat
     7561 gctgaaggat aagatatagt ctgtgcttgc a
//
LOCUS       DAMJVI010000145         7431 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.150, whole genome shotgun sequence.
ACCESSION   DAMJVI010000145 DAMJVI010000000
VERSION     DAMJVI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7431
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.150"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            61..1038
                     /locus_tag="PC545_003366"
     CDS             61..1038
                     /locus_tag="PC545_003366"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDF2458086.1"
                     /translation="MRDKIIEIILNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNIYYKLDTEFIGKNIEHLDTIDSTNEHAKRIAIN
                     SVDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNLGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IADKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNRELVKCLDINEEGNLLVQRSDGSIKEIISGEVSIRGEKGYV"
     gene            1031..1999
                     /locus_tag="PC545_003367"
     CDS             1031..1999
                     /locus_tag="PC545_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HDF2458087.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMVQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGSFKCRPDLKMGYRACEVANTDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLIVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKSIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKKREI"
     gene            2183..2773
                     /locus_tag="PC545_003368"
     CDS             2183..2773
                     /locus_tag="PC545_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDF2458088.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGAAIGFIFGISSLLNAITNPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            2792..3562
                     /locus_tag="PC545_003369"
     CDS             2792..3562
                     /locus_tag="PC545_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HDF2458089.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIISMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            3699..4667
                     /gene="dusB"
                     /locus_tag="PC545_003370"
     CDS             3699..4667
                     /gene="dusB"
                     /locus_tag="PC545_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDF2458090.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEEEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            4776..5255
                     /gene="greA"
                     /locus_tag="PC545_003371"
     CDS             4776..5255
                     /gene="greA"
                     /locus_tag="PC545_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDF2458091.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            5270..6799
                     /gene="lysS"
                     /locus_tag="PC545_003372"
     CDS             5270..6799
                     /gene="lysS"
                     /locus_tag="PC545_003372"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDF2458092.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMDIKDNFDSLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNVEDPRRTDRFEAFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            7108..7302
                     /locus_tag="PC545_003373"
     CDS             7108..7302
                     /locus_tag="PC545_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458093.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFEGTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
BASE COUNT         2963 a          751 c         1505 g         2212 t
ORIGIN      
        1 tgtataatta tttattagaa gaaaataagt catgaaatta tgtcatatta gaggtgaaat
       61 atgagagata aaataataga gattatatta aataataaat ctgaatttat ttcaggagaa
      121 gaattatcaa aacaattagg ggtgtcacgt gcagctatat ggaaacatat gaaagcctta
      181 aaagaagagg gatataatat agaatctgta aataaaaaag ggtatcgatt agcggaaaat
      241 ccaactgatt tattaagccc acagaatatt tactataaat tagatacaga gtttattgga
      301 aaaaatattg aacatcttga cactatagat tcaacaaatg aacatgctaa aaggatagct
      361 ataaatagtg tagatgggac tgtgatttta agtgaacatc aagatatggg gaaaggacga
      421 ttgggaagaa gatgggattc aaaacctcat gaaggaatct ggatgagtat tatattaaaa
      481 ccagatatgg aaccattcaa agccccattt ataactttaa tagcaggagc aagtgtaacg
      541 aaagctttaa acaatctagg agcaaataca ttaataaaat ggccaaatga tgttatttta
      601 aatggaaaaa aaatatgtgg tatattaaca gagttatctg ctgaaattga aagaacaaat
      661 tatgttgttc ttggaattgg aataaatgtt aaaataatgg attttgaaca agaaatagct
      721 gacaaagcca catcattgta taaagaaggg tatatgttat ccagagtaga tattgttagg
      781 aatgtattga ctgaatttga aaaattatat ttagattata ctgtaaataa ttccaaagaa
      841 aaaaccttaa aaatatgtag agattattct gcaattatag ataaaaatat atatgtatta
      901 aaaggagata atagagaact agtgaaatgt ttagacataa atgaagaggg aaatttatta
      961 gttcaaagaa gtgatggaag tataaaagaa attatttcag gtgaagtttc tataagagga
     1021 gaaaaaggat atgtataata atattttgga tatagaaggc ttaaaggttg ggcaggttga
     1081 ggatagaaat ggtcttactg ggtgcacagt agtaatatgt gaagaaggag ctacttgcgg
     1141 tgttgatgta aggggctctg ctccaggtac tagagaaacc gatttattag accctgtaaa
     1201 tatggttcaa aaagttcatg cagtagtttt ggctggaggt tctgcatatg gtcttgaatc
     1261 cacttgtggt gtaagtagat acttagaaga aaaaggaatt ggatttgatg taggtgtagg
     1321 taaagtgcct atagttgtag gagcagtttt atttgattta ggagtaggga gctttaaatg
     1381 tagaccagat ttaaagatgg gttatagagc ttgtgaagtt gcaaatactg atattttaaa
     1441 tcaaggaaat tatggagcag gttgtggagc cactgttgga aagataagag gtcaggaata
     1501 tgctatgaaa ggcggaatag gtagctattc tataaaatta gataatggtc taattgtatc
     1561 tgcattaatt gctgtaaatg ctcttggaga tgtttatgaa aatggtaagg ttatagctgg
     1621 tgctttaaat gataaaaaga cagaaacaat tgatacatat aaattaatga aacaaggtga
     1681 aagtaaaggt ggatttagtg tagataatac tacaattggg ataattgcaa caaatgctaa
     1741 gttaaccaaa gctggatgta aaaagatatc tcaaatggga catgatggat atgctaagtc
     1801 tatttttcct atccatacac ctcatgatgg agatacaata ttcacaatat caacaggaga
     1861 aatagaagca gatataacat tagtaggttc attggctgta gaggttgtgg aaaaaagtat
     1921 aataaatgca gtaaaaaaag cggatagtgt agaaaatata atggcatata aagatatagt
     1981 aaaaaaacga gaaatatgat gaaattagca tatttttctt gtcaaattgt tgacaatggc
     2041 aactcatctt gataaaatta ttcttgttag agcgggcatc ctacaatggt agcttgactc
     2101 atagttataa gaaaaaaacg aaaaaaatta aaaaattaag tagtccggca tcttaatgag
     2161 ctggaacgga ggtgtatttt ttatgatgaa cacaagtgca tcagtaagaa aagtaaatgt
     2221 aagaaagatg actataatag gagtactatc agctatttct attatgctat ctatgactcc
     2281 attgggattt attccagttg gacctacaaa agcaactata atgcacatac cagttattat
     2341 tggagctatt atggaaggac caatagttgg ggcagcaata ggatttatat ttgggatatc
     2401 aagtctctta aatgctataa caaacccaac agtaacatct tttgttttta taaatccatt
     2461 agtttcaata ttaccaagag ttatgatagg tgtattagct tattatgtat atcagttaat
     2521 aattaaggct acaaataagg tatacatatc aggtttaatt acaggggcaa taggttctct
     2581 gttaaataca gctggagttt taggaatgat atatgtctta tatgctgaca agtatcttca
     2641 tgcaatgggt aaaagtggtt cagcaggaaa agtaataatg gctcttgccg cagcaaatgg
     2701 ggtgccagaa gcaatagtag gggctttagt agtagctgct gttgccactg tacttaagaa
     2761 aagcaaaaaa tagaaagaag gttaatgaca tatgcttcta gtatttgatg ttggaaatac
     2821 taatatggtt ttaggtatat ataaaggtga caaattagtt aattactgga gaattaaaac
     2881 agatagagaa aaaacgtctg atgaatatgg aatcctaata agtaacctat ttgattatga
     2941 taatgtgaat ataagtgata ttgatgatgt tataatatca tctgtagttc cgaatgttat
     3001 gcattctctt gaaaactttt gtataaagta ctgtaaaaaa cagccattaa tagtaggtcc
     3061 aggcataaaa acaggtctaa atataaaata tgataatcca aaacaagttg gggcagatag
     3121 aatagttaat gctgtagcag ggatagaaaa gtatggagca ccaagtatac ttgttgattt
     3181 tggaacagca actacatttt gtgctatctc tgaaaaaggt gaatatttgg gtggaacaat
     3241 agcaccagga ataaaaatat ctagtgaggc gttatttcaa agtgcgtcta aattacctag
     3301 agtagaatta gctaagccag gtatgactat ttgtaagagt actgtatcag ccatgcaatc
     3361 tggaataatt tatggatatg ttggtttagt tgacaaaata ataagtatga tgaagaaaga
     3421 attgaattgt gatgatgtta aggttatagc tacaggtggg ttagctaaac tgattgcttc
     3481 agagacgaaa agtatagatt atgtagatgg ttttttaaca ctagaaggat tgagaataat
     3541 atatgaaaaa aaccaagaat aattaatata cagggaattg ggttatatat ccaattccct
     3601 ttttatgata gatttctagc tatatgtggt atattataat gaggaaataa gaatttaaag
     3661 ttaaatcaca aaataaaggc aaaaatagga gaaaaacaat gaaaatagga aatttagaat
     3721 tgaaaaataa agtttttcta tcacctatgg caggtgtaac agaccttcca tttagattaa
     3781 tttgtaaaga acaaggatgt gggcttttat atacagaaat gataaatggt aaggcacttt
     3841 gctatgatga tgaaaatact aaaaaaatgc ttaagataga agaggaagaa catcctgtag
     3901 cagttcagat attcggttca gaaccagagt ttatgggaag agcagcagag ataatgaatg
     3961 attattcaaa tgagattctc gatataaata tggggtgtcc agctccaaaa gtagttaaaa
     4021 atggagatgg ttcagcactt atgaaaaatc ctaagcttgc agaagaggta ttgagggcag
     4081 tagtgaagaa ctcaaaaaag ccagtgactt taaagattcg aaaaggctgg gatgataata
     4141 gtgtaaatgc tgttgaaata gcaaagatag cagaggattg tggaataagt gctttagcaa
     4201 tacatggaag aactagagaa cagttttata caggcaaagc agactgggat ataattgcag
     4261 agattaagaa aaatttgagt atacctgtta taggaaatgg tgatgtattt acaatagaag
     4321 attcaataaa tatgttagac aaaactggtt gtgatgctat aatgataggt aggggtgctc
     4381 aaggtaaccc ttggatattt aaaagaataa accattatat gaatacaggc gagattttgc
     4441 cagagccaac tcttaacgaa aaaataagta ctgccattaa gcatttaaaa cttgcagtag
     4501 aagaacatgg agagtatgta gctgtaaggg aaatgagaaa acacatcgct tggtatttaa
     4561 aaggacttag aaactctgca aaattaaggg atgaaattaa caaaatagaa gactatcaag
     4621 aagttgtgtc gaaattggag tactatatgg aagactcctt gacataaaaa taatagtgac
     4681 ttataatata gcgcataaat taaaaaaaga ttataaaatt tttaaaatat aattaggaaa
     4741 attcctaaga aatttgtgaa ggagttgtat ttataatgga agaaaataaa gagtttttat
     4801 tgacacaaga gggatatgat aaattagaag aagaattaga aaacttaaag gttgtaaaga
     4861 gaaaagaagt tgcagagaga ataaaagttg ctatatcttt tggtgattta tctgaaaatg
     4921 ctgagtatga tgaagctaaa aaggaacaag cacaagttga agaaagaatt ttaaaattag
     4981 aaaatatggt tagaaaagct gttatcatag atgaaagcaa aatagacctt aatgttgtta
     5041 caataggatc aatagttaaa gttaaggatt tagaatttga tgaagaggtt gaatatacta
     5101 tagttggttc gacagaagca gacccatatg atggtaaaat atcaaacgaa tctcctgtag
     5161 ggaaagcact tttaggtaga gctgcgaaag aagttgtgga agtgcaagtt cctgatggag
     5221 ttgctaagtt tgaaatatta gaaataagaa gataatttgg aggaaaaaaa tgaaaaataa
     5281 tcaacaaagc aatgaagaag cgcaaattca agaagacctt agtgaagtac ttcaagtaag
     5341 aagagataaa ctaaaaaaat tacaagaatc aggtagagat ccttttaaag aaagtagata
     5401 tgatagaact cattattcta tggatataaa agataacttt gattctcttg agggtaaaac
     5461 tacaaaaata gcaggacgta taatgagcaa gagaatacaa gggaaagctg gctttataga
     5521 tattcaagac caagagggaa gaatccaatc ttatgtaaga ctagatgcta taggagagga
     5581 agaatatagt gtattttcaa catatgatat tggagatata gttggaattg aaggagaaat
     5641 ctttaaaact aaaaaaggtg aaatatctgt aaaggctaaa tctgtagtat tactatgtaa
     5701 atctttacaa gttttaccag aaaaatatca tggattaaaa gaccaagaac taagatatag
     5761 acaaagatat gttgacttaa tagtaaatcc agaagtaaaa aatgcttttt taattagaac
     5821 aaaagcatta aaagcattaa gagcatactt agatgataga ggatttttag aagttgagac
     5881 tccaatatta aatactatag caggtggggc aaatgctaga ccatttataa ctaatcataa
     5941 tactttacat ataccaatgt atttaagaat agcgaatgaa ttgtatttaa aaagacttat
     6001 tgttggtgga tttgataaag tttatgaaat gggaagaatg tttagaaatg aaggtatgga
     6061 tttaaaacat aatcctgaat atactgcaat agaattatat caagcatatg cagactatac
     6121 tgatatgatg gaaataacag aaaatgttat tgcacatatg gctgaagttg ctacaggaag
     6181 tatgatagtt aactatcaag gaacagaaat aaactttaca ccaccttgga aaagaatgag
     6241 tatggaagat tgtgttaaag aatattctgg tgtagatttt tctactataa atacagatga
     6301 agaggcacta gaagttgcta gagaaaaagg tatagagata aaacctggaa tgagaagagg
     6361 agaagtaata aatgctttct ttgaagaatt tggagaagat aaattaatcc aaccaacatt
     6421 tataactcat catccagttg aagtatctcc attatctaag agaaatgttg aagacccaag
     6481 aagaactgat agatttgagg catttgctaa taagtgggag ttagctaatg cattttctga
     6541 acttaatgac cctattgacc aaaaaggaag atttatagac caattaagaa aaagagaatt
     6601 aggtgatgat gaagcttttg aaatggatga agatttctta aaagcattag aggtaggtct
     6661 acctcctaca ggaggactag gaataggtat agatagagta attatgttat taactaactc
     6721 tccatctata agagatgtat tattattccc gactatgaaa cctattgata acaatccaaa
     6781 taaagaagaa gaaaactaag aaatattagc attctactaa gttttaaata aagtttagct
     6841 attaaattat caataaatag ctaaatttta tttaaaatgt cagtgtagaa tgctttttat
     6901 atgcttcaaa gattctttta gatatttaat ttataaatta ccaacaaacg ttagaaattg
     6961 atattagttt ttgttgattg agactttata tttagaaaat atgattgaaa gtaatttatt
     7021 atgaaaatat ttagaataat ttatgagaag gtaaaaagaa taatttatga gaatactata
     7081 tataaaaaat tattggggga atgtctgatg tttcaagtaa ttaatttttt atgtttattt
     7141 tgtgttggat ttttggttct tgatgttatg atatattttt gtaaggatgc aatggatatt
     7201 tttgagggta caactaatgt taataaagtt aagagtataa atgtttgggc agatagagtg
     7261 agagctaatt catattctta tgaaaataaa attgcaaaat aggtaaaatt atatatgcat
     7321 gtattgtttg taatatcaag ggttatatct attagtttta attatattta ttaaaaaaaa
     7381 caaaagttat aacctttttt aaaataaaaa acaaaaaaag tattgacgaa a
//
LOCUS       DAMJVI010000146         7404 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.151, whole genome shotgun sequence.
ACCESSION   DAMJVI010000146 DAMJVI010000000
VERSION     DAMJVI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.151"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(62..1009)
                     /locus_tag="PC545_003374"
     CDS             complement(62..1009)
                     /locus_tag="PC545_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2458094.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            complement(978..1424)
                     /gene="lspA"
                     /locus_tag="PC545_003375"
     CDS             complement(978..1424)
                     /gene="lspA"
                     /locus_tag="PC545_003375"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2458095.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            complement(1734..2513)
                     /locus_tag="PC545_003376"
     CDS             complement(1734..2513)
                     /locus_tag="PC545_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2458096.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            complement(2568..3050)
                     /locus_tag="PC545_003377"
     CDS             complement(2568..3050)
                     /locus_tag="PC545_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2458097.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            complement(3316..4737)
                     /locus_tag="PC545_003378"
     CDS             complement(3316..4737)
                     /locus_tag="PC545_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2458098.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            complement(4883..5653)
                     /locus_tag="PC545_003379"
     CDS             complement(4883..5653)
                     /locus_tag="PC545_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458099.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            complement(5658..6860)
                     /locus_tag="PC545_003380"
     CDS             complement(5658..6860)
                     /locus_tag="PC545_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2458100.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
BASE COUNT         2473 a         1279 c          718 g         2934 t
ORIGIN      
        1 ttcactaaat ttatttactt atataaaaac aaaaaatgat ttataagtat tatacaaata
       61 gttattttat ctttttaata acttccttaa aatactcagg taattctgaa ttaaattcga
      121 tatattcctt agtagtagga tgtataaatc ccaacttctt agcatgcaaa gtttgtcctt
      181 ttaatttaaa tttagtttct tttggaccat atacataatc acctaaaagt gggtgattta
      241 tagacaaagc atgaactctt atttgatgag ttctaccagt ttcaagccta gctctcatat
      301 gtgtaaactt atcaaatctt tctacaactt caaagtgagt gattgcattt tttccatctt
      361 ttactacagc catttttaat ctatccttag gatttctccc tataggttta tcaacagtta
      421 ttttatcttc tttaaccaca ccatagcata taaattcata ctccctagtt atagtatgtt
      481 cctttaactg ttctgctaga aaattatgtg cataattgtt cttagctatc ataagaagac
      541 cagaagtatc tttgtctatt ctatgaacta taccaggtct aataacgcca tttattgatg
      601 acaacttatc tttacaatga tataaaatcc cgtttactaa cgtattttca tagtttccag
      661 gagcaggatg gacaaccata tcctgaggtt tatttattat taagacatca tcatcttcat
      721 atacaatatc tattggaata tcttgaggta aaacttctaa tactttaggt tctggtatct
      781 ctataacaat attatcttcc tctttaacca gatatctagc tttttctact ttattattaa
      841 cctttacttt tccatctttt ataatttttt gtatataact tcttgacata tcacctaatt
      901 gttctgctag ataaatatca agtctaactt cttcttcctc ttcaagtact aaaaactctt
      961 ttacttcatc cattatatca cctactttta ctttcaaaaa ataaaacata tatacataaa
     1021 aacacagttc ctacaactac aaatacatca gctatattga atacatattc ccatataatt
     1081 ctaaagtcga agtaatctac tacaaaacct aatcgtactc tatcaattag atttcccaat
     1141 gcaccagata taattaatat aattcccaac cttccaaata tatgtacttt ccttgtatta
     1201 agatagtata gtccaaatac tgttgcaagt aatgcaacaa ttataaatat ccattgatta
     1261 ttttgtaata atccaaatgc tgcacctcta ttttcgacat aagttaaatg aaatacatta
     1321 tttattattg gtattgttga tacatccacc aaattattca atacccatat tttagacagt
     1381 tggtctaaac ctatgagtag aattattatt aatatatata gcaatgattt ttccccctat
     1441 aaattattta aaaaccaatt ttatttgaag cacactatta ttattataca aaaaaaatgc
     1501 tctaaagagc aactttatta aaattgtatt ttcttaataa agctagaaca aaatttaaaa
     1561 tagtactact ttttttatat tgtagatttc tacatattta aaatcatatc attatgtata
     1621 aaaaatttaa aaataccata ctaatcaata ttttttgtgt attttataga ttaaagatta
     1681 atgatgtctg taatttaaac ttacaatcac cactaatctt caaacaaata tttttacttt
     1741 tgcacttgct tatttttatc taaatgttgt ataagttcag atactgttac gaatttatat
     1801 cctctctctt ttaatttagg aattactact tctaaagctt gtatagtttg tgatttatca
     1861 tttcttttag tgttataatc atgtaaaagt attatattac cattttggac ctttgtaagg
     1921 atagtattta taattgcatc tactccaggg ttagaccaat ctctagggtc taagtttgac
     1981 caaagaatta tattcatgcc tttagaaact ataatatcac agacactttt actcatggct
     2041 ctatatggag gtctaaatat ctttggttct actcctatta cactttttat aatattttgc
     2101 gtatctgtta tttctttttc aacactacta taactatttt ttgcaacatt tatatgtgta
     2161 aatgtatgat tccctatttc atgcccctca tcatattgtc tttttaataa ttctttgtta
     2221 tattctactt tttctccaac tacaaaaaaa gttgctttta catcattttt ctttaatata
     2281 tcaagtactt gtggtgtaaa atcctcatct ggaccatcat caaaagttaa tgctaaaatt
     2341 ttttcatcct catttccttt ttttattatt acatcttcat aatcgtcttt tatattgtca
     2401 tttaaactat atgcttcttt tgaactatct tgattatgtg tattatgatt atatgtatat
     2461 acacctacta tagataataa acatatacat ataccccata ttattttctt catgtcatta
     2521 cctcttttct aattcattct agcaatatat tttataatgt cagtttttta atctctcaat
     2581 attccatttt tatttaaatc agttaaattt gttaggtctt ctgaataaaa gttagaatct
     2641 tctacatcag ggttttgcat tgcatcacct aataaattat tttctttctt tgcacaatca
     2701 ttacataaac ttgtatctgg caatatatcc aatctttcta aatctatttg attatgacat
     2761 aaatcacaaa tcccatatgt tccattctct attttagata atgctaagtc tatttgatac
     2821 aatcttccct cttcatgatt tattagactg ttttgcatat tttgcatata taaatctgtt
     2881 cccatgtctc ctatatgatt atcataacta gataattctc ctgatgaata cttttcactt
     2941 gtatgtttag ttgtatctcc aaataaagta ttatctttca tatcctctac taaattagtt
     3001 aaattttctc tttctttcaa taatttatct ttatattttt tagtatccat ttttaacacc
     3061 tctaatttta atatttacat cataaaaata gtatgtggaa aagtaaatta aacatacaat
     3121 aaaaataaga tttgattcga aaaatggaat gtctcaaaat gaaattttta gttcatttaa
     3181 ccctcttttt tatatgaaat caaatatatt tttatccttt cagcaataaa aaagagactt
     3241 atctctattt tgagatagtc tcttctttga acataattta aatacaattt tttaattttt
     3301 atagttatta ttttactaac ttgccaattt atgattattt tgttctttaa tatgttttgc
     3361 ttttttaaag aataaaagac caagaattat tgctacaata acacctattc cattagatat
     3421 attaactggt agtctaaatc cttcttttgc ttgcattata taactaaatg ttactacagt
     3481 cataaaaagt gctggtaatg tagttatata atggttttta tttgctttga ctaaatatgc
     3541 agaaccagtc cacaacatta tcattgcaag tgtttgattt gcccaagaga aatatctcca
     3601 aattatatta aaatctataa atgtaagagc aacaccaact gcgaataatg gtaaagctat
     3661 taaaaatcta tttttgactg catcttgatt ataattcatt gcatctgcta ttgtcagcct
     3721 agcacttcta aatgctgtat ctccagaagt tataggacat gccacaactc caagtatagc
     3781 taaagctcct ccaataggcc ccattaatcc aacagatact gtatttacta ctgttgctgc
     3841 tccaccatca ttaaatatca cattaagttg aggtatgcca tggaaatatg acatggctgc
     3901 tgctgcccaa actaaagcta ttataccttc tgcaaccatt gacccataaa atactggtct
     3961 tgcctcactc tctttctcaa cacatctcgc cataagtggt gattgagttg catgaaaacc
     4021 tgatattgct ccacatgcta ttgttataaa taagtatggg aataaaggtg ttccatttgg
     4081 attcatattt tgtaaattta tatttggtat gttatacccc tttattatta atcctccagc
     4141 tacacctact gccataatta ataatgctgc tccaaataaa ggatatattt taccaatcaa
     4201 tttatctatt ggtaaaacag tagccataat ataataaagt attatcactc caacccatat
     4261 ttgtgcgttt acacctgtca atgtctttaa taatcctgct ggacctgtta caaatacaac
     4321 tccaaccaat actaaaagaa taacactaaa tattctcatt gctatttttg caccatttcc
     4381 tagataaagg cctaccaact ctgatgcaga tgtacctttg tttttcattg atagatatcc
     4441 tgaagcaaaa tcgtgtactc caccagcaaa gattgttcca aacacaatcc ataaaaatgc
     4501 tgatggtcca aataatgctc cttgtatagc tccaaatatc ggacctaacc ctgctatatt
     4561 tagaaactga attaaaaaga ttctcttcca tttcattggc acataatcta ctccatccct
     4621 acaagctata gctggagttt gatttttgtc attggttcca aaaacctttt ctacaaaagt
     4681 accatagaca aaataaccta ctataagtac tactattgaa cctaaaaagc ttaccataaa
     4741 ttcatcccct tagtttttat ataattgctt tataaatttt tatgagttta agaaaactat
     4801 ttccagttca aatagtaata cttttttatg ctaatataaa tattaattac attaagtgca
     4861 tatttaagta catgaaatga ctttatatat gcattaacag cctaaattct tttactttgc
     4921 ttctacttac agttacttca taatcactat tttttaattt taatatgtat gtattattaa
     4981 accatggtat aatttctttt actttggtta agtttacgat ataagaccta tgtgttctaa
     5041 aaaatgtttt atcatttata agattttcga cttctgatat gccttctttt aatataaact
     5101 tttctttctc agtatatata aatgtctgcc tttcattagc ttcacagtaa tatatatcat
     5161 caatatctat tacaactaat ttatcacctt tccaaaggct aattttatgt gttatttctt
     5221 ctatctcctg atttactgct tccttcttat atttgtataa attactattt acattaaata
     5281 gttcaatatc actcatctca cttttttcta atttattcag catagaaata attctctctt
     5341 cagaatatgg tttcaatata taatcaaata catccaaaga aaaagcatct actgcataac
     5401 tttcatatgc tgttataaaa acaattttag gtcttgattt aaactgattt aatgtttttg
     5461 caagtagcat tccatcaaga tgaggtatat ttatatctaa aaaaattaca tctattttat
     5521 tttcctgaat aaagtctaac acctctatac cattggtaaa ttcacttact acctcgattc
     5581 cacttttatt ttcaataaaa tatctcaatt cctttcttgc tggaaattca tcttccacta
     5641 ttatagctct catacttcta ctcctttaca taaaatacta tcaaagtccc attatctatt
     5701 cttcttatat taagccctct tccatacatc agtttaagcc ttaaatgtac attataaagc
     5761 ccaatcttat tttctggcat attatcttga tatacattat ttataatttc ctcacttata
     5821 cctataccat tatcctctat tgatattctc acagtatttt ctaatgaata cggtaacttt
     5881 tttatggata ttttcactac accagctcta ccactttcta aaataccatg tattattgca
     5941 ttttccacta atggttgtat aatcaaactt ggaatcttta tatttacatc ttctatatca
     6001 taagatacag ttagtaattc tccaaaccta gctttttcta tctctacaaa agatttaact
     6061 tgttcaatct ctttatttat atcaattaag ttatcactat attcaagatt atatcttaga
     6121 taagttgata aatttattat taactctcta gccttatctg gactaaatct tataaatgaa
     6181 gctattgtgt ttagggaatt aaacaaaaaa tgtggattaa tttgtctttg aagtgctttt
     6241 atttctgctt tatttctcgc ttctatatct tttttatctt tctcaatccc ctctactata
     6301 gatataacaa atcctattcc aatctgccca actattaaag gtaaatatat tccaccgatt
     6361 atttgaattg ctaaagaatg tggatatgat attaagtata taagtaatat agtaaaactt
     6421 tcagaaatca tgcctaccaa tattccatac attactctat gttgttttgg tatcctctta
     6481 tatagaaatc ctgataatac acctgctagt atacttgata aaagacatgg aacagatgtt
     6541 atccccccaa tatccataaa atatctatga atccctgaaa aaactcctgc tgtaatacct
     6601 accatcggac caaataaaat gccacctgat actatagata ttattctagt gtttactatt
     6661 gaccccatat aatttatacc actataagtt ccaaatatgg ctaaaaaagt aaaaactaat
     6721 gctatacaga ccaagtcatt aaaactatat tcttcctttt gaaatatctg cttaaatatt
     6781 tttaactttg ttattagaag aaacaatact acaataaggc ttgtcttttc tattaatgcg
     6841 attaaaattg atgttttcat aatctccctt taaaaaataa atttttacac actctaaatt
     6901 tattatggtt gttataaatt acatatttga agtaatccta tcacaaaact ttccacattt
     6961 aatcttttaa ttaaataaga aatagttgta cttctattaa caactaatac tagctagcat
     7021 aagcttgtaa taacttgtag ttattattaa ctttacaatt ctagtattta attttatact
     7081 ttagaaatct cttttcttag aaataatact ttcgaatatc atataataca atattatgta
     7141 gattcttact catacaacat atttctttaa aaattttata ctattctttg ttcaaatatc
     7201 tcatttgatt taattgtgtt aaacacacat tatcatctct ctgttatata tttattatat
     7261 agccgtatat cgataaagta aataaatccc atatttaaaa taatatgttt gacaataaat
     7321 tattataatg tatatttacc ataataattt gtcaaccttg aaagttactt ctataattat
     7381 tgttaaataa ttcttctaaa aaaa
//
LOCUS       DAMJVI010000147         7215 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.152, whole genome shotgun sequence.
ACCESSION   DAMJVI010000147 DAMJVI010000000
VERSION     DAMJVI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7215
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.152"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(22..684)
                     /locus_tag="PC545_003381"
                     /pseudo
     CDS             complement(22..684)
                     /locus_tag="PC545_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(844..1380)
                     /locus_tag="PC545_003382"
     CDS             complement(844..1380)
                     /locus_tag="PC545_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2458101.1"
                     /translation="MIGLKCGMVKLLEHQMIWDKSAKDVIILLKSIWDKTAIDIQHIG
                     STSIPSISAKPIIDIVVGVASLEEAKLYLERLEQCGIVFRGQDVPKQLLFAMGDFEKN
                     TRTHHIHVVEWNGVAWNNYINFRDYLNTFTDKAKLYDSHKQKLALQFEDNRKIYTQRK
                     QELINILLEEARLWRINT"
     gene            complement(1453..1866)
                     /locus_tag="PC545_003383"
     CDS             complement(1453..1866)
                     /locus_tag="PC545_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2458102.1"
                     /translation="MKMPKESIDTIMFAPCGMNCMVCYKHCYHKKPCAGCLNGDTGKP
                     EHCRKCKIKDCVCQKGLFYCFECFNFPCKLIKNLEKSYNKRYQASLMENSKFVQQYGL
                     EKFMQKQKERYTCSKCGGIISIHDRECSECQEKVK"
     gene            2332..2949
                     /locus_tag="PC545_003384"
     CDS             2332..2949
                     /locus_tag="PC545_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HDF2458103.1"
                     /translation="MDEKILELLQEMQGSISGIEGTISDMQSNINGIEGTISDMQSNI
                     NGIEGTISSMQGNINGIENTISGMQGTIGDMQGSISGMNGTISDMQGSISSMDGTINS
                     MQDSITDMQVEIKEVNSKLDNMEPDIKDLKTGQVETSSRLDTIQSDVTEIKSDVKINR
                     NTLDTVIKHTSSLVKDTSDIKNDLNNIEIVTSKNSNDIAKLKSVK"
     gene            complement(3557..3736)
                     /locus_tag="PC545_003385"
     CDS             complement(3557..3736)
                     /locus_tag="PC545_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2458104.1"
                     /translation="MLQTGKQGADKKYLDNMFFVKGYVEELLFLDNNYNIVFSCIVFH
                     HCTNVQNIFCIFWKW"
     gene            complement(3962..4495)
                     /locus_tag="PC545_003386"
     CDS             complement(3962..4495)
                     /locus_tag="PC545_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDF2458105.1"
                     /translation="MQYISYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGKEPDFTPSLHMVGTPFQTTVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIIPCHRVVGTNGSLTGYAGGINRKIKLLSL
                     EKVSVQHYFIPKKGSAL"
     gene            4812..5162
                     /locus_tag="PC545_003387"
                     /pseudo
     CDS             4812..5162
                     /locus_tag="PC545_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729451.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5916..6488)
                     /locus_tag="PC545_003388"
     CDS             complement(5916..6488)
                     /locus_tag="PC545_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2458106.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGNCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            complement(6550..6813)
                     /locus_tag="PC545_003389"
     CDS             complement(6550..6813)
                     /locus_tag="PC545_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2458107.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESKYGALTNFGYESSEYPWSWISEPWPWDKSFYK"
     gene            complement(6813..7034)
                     /locus_tag="PC545_003390"
     CDS             complement(6813..7034)
                     /locus_tag="PC545_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2458108.1"
                     /translation="MGYLKEDKQDYCCEFARPYINRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
BASE COUNT         2542 a         1225 c          872 g         2576 t
ORIGIN      
        1 agtgtgcgta gctatttttt aatcaaactg gaatacagac tccattccta ggaaattggc
       61 tgaaatatag tctgctgtca aagatgctgt catcacagaa attaaaacag atactgaaaa
      121 gtttttatgg acttcttcaa gtgaaaacat aacacccgcc agtggtgcat gaaaggctgc
      181 tgataatcca gcacttgccc cacaggttaa caggaatttt tcctctgtct tccctcggtc
      241 aagagctcta gaaatacctt tacctgccat agcaccaagc tgaatggaag gaccttccct
      301 tcccaaagca agaccaccca tgagtgataa gaatcctcca ataaattttg ttggcaacac
      361 tcgccaccat acttgtttta ttttccctgt catttccccc tcaagctgag ggataccact
      421 tccagaaatc attggctccc agtccaccaa ctttgataca atccatgcaa gtactgcaag
      481 tactgcaaac caagctatta tttttattgt agaaccttct acaaatttta aaatttggct
      541 aagccaagta ccagcctgcc ctaaaagtat cctgtataaa agcacaatca aaccagccac
      601 agccccaacc ataagtcctt caccaatcaa aattacctga aagtgctctg cccgtttgat
      661 aatatgagat gtatcctttt tcatactgtt cctccttcat ccaataattc tacacaatct
      721 gaatctccaa tgactaaagt catgagtgtg tacagatatt ttttaatcaa caaaaataga
      781 tttttattta ctaaatataa gaaaataatt gctcaatttc tcaataaatg taaatttatt
      841 cttttaggta tttattctcc acagtcgtgc ttcttccagc aaaatattta ttagttcttg
      901 ctttctctgt gtatagattt tacgattgtc ctcaaactgt aatgccaatt tttgtttatg
      961 actatcatag agttttgctt tgtctgtaaa agtatttaga taatctcgaa aattgatata
     1021 attattccat gcaacaccat tccattcaac aacatgaata tggtgagtac gagtattttt
     1081 ctcaaaatct cccatagcaa aaagtaattg ttttggtaca tcctgacctc gaaaaacgat
     1141 tccacattgc tctaaccttt cgaggtataa tttagcttcc tctaaagaag caacacctac
     1201 aacaatatca ataataggct ttgcgctaat actgggaata gaagtgcttc caatgtgttg
     1261 aatatcaatg gcagtcttat cccatatact tttcaataat ataatgacat cttttgcact
     1321 tttatcccat atcatctgat gttccaataa tttaaccata ccacatttta aacctatcac
     1381 tatataccac ctcaatataa tccaccttta tatatattac aattttttct ctcttgataa
     1441 acagtgattt agctatttca ctttttcttg acactcgcta cattccctat catgaataga
     1501 tataattcct ccacatttag aacaagtata tctctccttc tgcttttgca taaacttctc
     1561 taaaccgtac tgctgaacaa atttactatt ttccataagg cttgcttgat atctcttgtt
     1621 ataacttttt tcaagatttt ttatgagttt acaaggaaaa ttgaaacatt caaaacaata
     1681 aaacaaccct ttttgacaga cacaatcctt tattttacac ttacggcagt gttctggctt
     1741 tcctgtatcg ccattcaaac aaccagcaca tggctttttg tgataacaat gcttatagca
     1801 aaccatacaa ttcattccac aaggagcaaa cataatcgta tcaatactct cttttggcat
     1861 tttcataaca ttttactcct ttcaaaaatc caatttgtcg aattgttata tattcatatt
     1921 gtaacataaa aaatagctaa aaaattcatg cactttcatt gccaataaat tttcaaggaa
     1981 atatgaaagt ttaaaactaa gaataaaaag agataaaaag gaagctacta atcaaattat
     2041 tcaaatacaa gtaatcaata taaataactt aaggtatatt taaaagtata tgatattacc
     2101 agcagtttta aatacaaata taaccacaca tgcctcaaaa acacttatta cagtatctta
     2161 gaaacttaga caatacataa gattcattaa taaaaaagtt ctcaatatag atatatttaa
     2221 ccatgttttg aatagcagat aatcatatga ttaaaaatta aatatgacat agcgtttgtg
     2281 gaatataaca cctaaattat gttataataa taaaacagga gtgtgatagt gatggatgaa
     2341 aaaatattag agttattaca ggaaatgcaa ggtagtatca gtggtatcga aggcactatt
     2401 agcgatatgc aaagcaatat caatggtatc gaaggcacta ttagcgatat gcaaagcaat
     2461 atcaatggta tcgaaggcac tattagtagt atgcaaggca atatcaatgg tatcgaaaac
     2521 actattagtg gtatgcaagg cactattggc gatatgcaag gtagtatcag tggtatgaat
     2581 ggcactatta gcgatatgca aggcagtatc agtagtatgg atggcactat taatagtatg
     2641 caagacagta ttactgatat gcaagttgaa attaaagaag tcaatagtaa gttagacaat
     2701 atggaacctg atattaaaga cttaaaaact ggacaagttg aaactagtag tagacttgat
     2761 actattcaga gtgatgttac agaaattaag tcagatgtaa aaattaacag aaatacatta
     2821 gatacagtga ttaaacatac ctcttcatta gtaaaagata catcagatat taaaaatgat
     2881 ttaaataata tagagatagt cacatctaag aattcgaatg atattgctaa attaaaatca
     2941 gttaaataat ataccatagt aaaaaatacc tgaaaatttc acatacttct ttaaagttat
     3001 accaaatttt atacagaaat actgattatc cgacaacaca ctttacttat aaataattta
     3061 ttaaaaaaat actcatttta tcctaaaatt aacacgacta aatgagtctt gatataataa
     3121 taaaaaataa gggagattgg acactaaaaa gttgtgtatc tcccttatat cctataatat
     3181 agttatataa tttttcagcc taaaataaaa gtccatcttt gattaaaaca catcccctta
     3241 tcacttataa aaggataaaa tcttgttttc atttttccat ttatatcttc ttgcttaaga
     3301 gtaataattt tttcttttga tactttaagt gtttggttaa atttaactaa tcgactaagt
     3361 tttgttcaac atctattttt catattttcc aatacttaat ctatttttta taaataattc
     3421 tttcatttct aactcactta cattttttac ataaaacgaa acatataaat ttcaacctta
     3481 tcttgtatgg ttcttaatac ttaatgagta gcttaagtat caatcattat aaatttttcc
     3541 cttacattta acactcttac catttccaaa atatacaaaa tatattttga acatttgtac
     3601 aatgatgaaa tacaatacat gaaaatacaa tattataatt gttgtctaaa aatagaagtt
     3661 cttcaacata acctttcaca aaaaacatat tatctaagta ttttttgtct gctccttgtt
     3721 ttcctgtttg tagcatataa attacttcct ctaaacatat aactactcac atgaaaaaag
     3781 aaaataaact tccaaaatac aagctctaaa aactacctct aaagttgtat aatgtttact
     3841 aggaataagg ttgtataaca tatagtttaa ataatcattt tttaaaattg caagttattg
     3901 ctttcaaaat tagatgtttg tatatcaaat aaatgattaa gaatttctaa atttaatggc
     3961 tttataatgc actccctttc tttgggataa aataatgttg cacagatact ttttctaaag
     4021 ataacaattt tatttttcta ttaattccac cagcataccc agtcaaactc ccatttgttc
     4081 ctacaacacg atgacatgga ataataatag agattccatt atgccctact gcaccaccaa
     4141 ttgcttgtgc tgacatttta gatattcctt tttgttgagc aactttatat gcaatttctc
     4201 cataagtgat tgttttacca tatggtattt gttgtaaaat tttccacact gtagtctgaa
     4261 aaggagtacc taccatatgg agtgatggtg taaaatcggg ttcttttcct gaaaaataaa
     4321 tatctaacca tttctttgtc tgctcgaaaa ttggcaattc tttttcttga tgttcttggt
     4381 caagattata agcataatac ttttgattct caaaccaaag accagttaac ccaatattat
     4441 ctgatgctaa aagaatattt ccaattggac tatggtaata gctaatatat tgcatacaat
     4501 tcctccttat attctgaaaa cttcactcaa atcattagac acttcttttc tatctaccat
     4561 tatgtaaaaa catactaata taatttcctt ctaactacac tttcgttata atatctatcc
     4621 tcatatcata ttagttttat atttacctgc atctgttcat ccttttttcc ctttcccttt
     4681 gatggatata aaattcatac atttaagctt cctactgtat taacttttat agaagtgtat
     4741 cttataattt tatcactaaa tagaccaaaa aaatatcgat atagcatcaa gtaatatcat
     4801 aatactttca aatgggaaaa gtaagaatag gctgtaaaaa agaaaagtat ttttatgtta
     4861 gaaatccagt aaaatcgtta gtagatagaa taacagaaaa cgaacaattt gctatttatc
     4921 attttacaca taaatataat tgacgcaata aagtgttttc tattctacaa cgattaataa
     4981 ggttagaaaa aagtaaaaat gaactgtata tgtatgaagc attagtttct gtttctttcc
     5041 tttggcttat tatgataaaa aatattgttt tgccaccaat aaaacacaaa aatataatta
     5101 atatccatat gcaaaaaatt ttacgttata ttgaaaaata ctattgtttt acattatatt
     5161 gaagggtact attgtgaaaa cttaattttg gagtactatc aagcagtggt aatatcaata
     5221 aatcagagtg ctccagatga tttgaactaa gtttaaatac tataccttat aaatatttta
     5281 ctgagttttg tttattaaaa gcaactcaat ttctaaaagg cacaaatgag ctaataagtg
     5341 acattactat aaaagttgtt tttcatcaaa taatctattt tagtaaatgt tttaaagaaa
     5401 aaacgggata ttctccaaaa gtgtacagaa atataaaaaa agagatatga ttttttattt
     5461 cttttgatta tccaaagagt aaataaaaaa tattgtaaaa aacttactaa caacggcaat
     5521 gctttgaact atggtaatac tttgaacttt tgattggact ttttttgcta aaataatata
     5581 tttaaatagc aaagtaatct gtttaatcaa taaaaaataa gagataaaag aaatcaacta
     5641 aaacttttac ctcttatttt gatagtttat cagagctaat atttacactc tttttatttt
     5701 tgatacttca ttaaaaatga tgcttattct cttttcattt ttaatacttc attagaactt
     5761 atgcttattc tcttttcact tttaattctt cattagaact tatgcttact ctccattcac
     5821 ttttaattct tcattaaaaa tgatgcttac tctccattca cttttaattc ttcattaaaa
     5881 atgatgctta ttctccattc actttcaatt cttcattaaa actgatgctt gcactctgta
     5941 aactcataaa catccattaa tgcttcacca aatctttgat agtgaactac ttccctctca
     6001 cccaaaaatc tcaatatatc tttcaaatca gggtcatcag ttaaatttat taattgatag
     6061 tacgtagcca atgctttttg ttcagcagcc atatcctcat gtaaatcagt taccgaatcg
     6121 ccaaagacat taatataact tgtagtaaag tttactccat tagaatctac tggctgaaga
     6181 ccatgtccaa aattagcata attactacca agtccagcag ccttaagctc ttctggagta
     6241 caattgccta ttaattgata taacactgaa gaaattatct caacatgagc catttcctct
     6301 gtacctatat cagttaaaag tgcacgagat tttccagttg gcatagtata tctttgttga
     6361 agatatctag ttgcagctgc caactcacca ttaggtcctc ccaactgagt catgatatat
     6421 tttgccattc tagggtctgc ttgtcttatg ttaactggat gttccagtgt tttttgataa
     6481 atccacatat tatcttactc ctccttaata tatatttaat aataaatcta attttaaagt
     6541 ttaataattt tatttataaa atgatttgtc ccatggccaa ggttcagata tccatgacca
     6601 aggatattca cttgattcat atccaaaatt agttaatgct ccatatttag attcataatc
     6661 acacacagct tgtgtaaatt tatcgcagta tctgttgtat gaatcaagag ctttcttatc
     6721 ccttggattg ttatctaaat ataaatttaa ttctatacag gcaaattgat attctgaaat
     6781 tctctctaaa agttctcttc tatttaatga cattagtaca tgttaccccc ttttttagct
     6841 ctaactctat ttttaggatt tgcatatagc caatcattat agttatttga ctctattaaa
     6901 tctaattcag gaaataaagt acctcttttt aatgctgttt ttggattgta tgtgtcatca
     6961 taaatttgac gattgatgta tggtctagca aattcacaac aatagtcttg tttatcctct
     7021 tttaaatatc ccattaatta tcctccttaa tttttagaat tttaaaatag cattattttt
     7081 aaaaattgta tactggttct atattataac tggtagtttt aataggtctt taataagatt
     7141 aaatgattaa ataagtatct ttttttctta ttttacctct aaaatttttc taattaatct
     7201 ttcataaata tctat
//
LOCUS       DAMJVI010000148         6905 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.153, whole genome shotgun sequence.
ACCESSION   DAMJVI010000148 DAMJVI010000000
VERSION     DAMJVI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.153"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            95..1837
                     /locus_tag="PC545_003391"
     CDS             95..1837
                     /locus_tag="PC545_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HDF2458109.1"
                     /translation="MVKSKKKIFVFLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSDEFYSKEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLAKSTVSIDGIEVDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKIQ
                     PSIDVCEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLNDINKLFFDLEEEYGVSNNILREKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQEDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQNGNLEEISSTLYD
                     YFGIDNPLELKNAS"
     gene            complement(2001..2195)
                     /locus_tag="PC545_003392"
     CDS             complement(2001..2195)
                     /locus_tag="PC545_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458110.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(2192..2773)
                     /locus_tag="PC545_003393"
     CDS             complement(2192..2773)
                     /locus_tag="PC545_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2458111.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCITNGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEDVDVR"
     gene            2941..3759
                     /locus_tag="PC545_003394"
     CDS             2941..3759
                     /locus_tag="PC545_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2458112.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IETTIKRIGGNDIMKNYDVTIKNFAPKYMMTLRRVIPTYQDEGMLWHQVFLETKDQNV
                     QIEPPKYSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGNWISDNNYDVDGPMFNIYHVSPGNDSNPDNWVTEVCFPVKKK"
     gene            4769..5590
                     /locus_tag="PC545_003395"
     CDS             4769..5590
                     /locus_tag="PC545_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallohydrolase"
                     /protein_id="HDF2458113.1"
                     /translation="MKLKIVGSGGMSIIPSSFCKCKICEEARQKGGRYERLGPSLYID
                     DIKMLIDTPEDIAVACNRQKISEVKHISISHHDPDHVKGIRIVEKIGCDFITGENNPI
                     GFYALPEVIEDINRTNLDCLKYYGDVINCISINETSHVKINNFDIDLINNNPDRNITF
                     YVIKENEKKVIYACCNPKPFTHSDMYFDTDVLIISLVSDDGILNDGTKLKDTPFKDEI
                     FTLDEIVEIKNYYRIKKIIITHIDEMWGKSYGYYSELEKKLDNIYFAYDGMEIIV"
     gene            complement(5900..6679)
                     /locus_tag="PC545_003396"
     CDS             complement(5900..6679)
                     /locus_tag="PC545_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HDF2458114.1"
                     /translation="MSYKKIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKIFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIMNISSQASFQPLPYQNIYSSTKAFIRNY
                     TRALNVELKSKGINAIAVCPGWMSTNLFKRGIVVAEKGTKNFPGIVSPDIVAKKALKD
                     AKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2592 a          914 c          877 g         2522 t
ORIGIN      
        1 atctatatct ttatgttaaa ttttcttaac atgcattttt tatatttaat atagtaaaat
       61 aattaataac tatatccatg attggaggat ttttatggtt aaatccaaga agaaaatatt
      121 tgtttttcta attttaattg cagtttttat tatatttgca tcatacataa gaaattatac
      181 taatgataca aaaaaagatt ctgttagagg aactattcct tttgaaaaaa tggaatataa
      241 gcgtcctaat atacagtcaa tttgcgaaaa cattaacaag tgcaacgaca aacttttaac
      301 agctaaaaca gctaaggaac aattaaattt atttaatgaa gttgataaaa tttatcaaga
      361 tttttacagt acacttacta tagcaaaaat tagaaataac atagattcat cagacgaatt
      421 ttactctaag gaatataagt atcttatgag taaatcagta gatgttgaca tgtcatacaa
      481 agactttcaa gatagatttg taacttctaa attctctaaa gaattagaaa aagaacttgg
      541 aaaggataca tttaattatc taaagaacat tggaaaactt aattctaaag aagttgaagg
      601 tttgcttaaa aaagagcaag acctagttgt aaaatatgaa gacttattag ctaaatctac
      661 agtatcaata gatggtatag aggtagactt tgaagaagcc ttgtcaagac caaatcttag
      721 ccctgaagaa tatgtgaaaa tttattcaga ttacttaaaa aaatacaatc ctatatttgg
      781 taacatattc ttagaactta tacagacaag aactgaaata gcaagtaagc aaggatttaa
      841 gaattatata gattatgcct atatgaacct aaacaaagac tattctcaag aagaagctaa
      901 aaaatttaga caagacgtaa aagattacat agttccatta tatagagaga tttcatctaa
      961 accatctgat tcttctattt atattaaagt atataaaaat cgctcattta ggaagtttga
     1021 taaagtactt gaagatatat ctccaaaact aaaagagagc ttcgactata tgaaaaaata
     1081 tgacttgtac gattattctt ctggaaaaaa caaatcacct ggaggatata ctacttatat
     1141 aaataaatat aaagcacctt tcttgtttaa tacatgggac aattcttttt taggtgttac
     1201 tagttttgct catgagtttg gtcattttta taattactac aacagtatta acttaaataa
     1261 taagatacaa ccaagtatag atgtatgtga agttcattca acatctttag aaattctatt
     1321 ttataaatat tttgatgatt tttttggtaa acaatctgaa gctataaaaa aagaacactt
     1381 atcgattgtt ttaaatacta ttattgatgc ttgtctttat gatgaatttc aagaaattat
     1441 atacaagaat ccatatatga gtctaaatga tatcaataaa ttgttttttg atttagagga
     1501 agaatatgga gtttccaata atatacttag agaaaaaaat gctccttttt ggatacttgt
     1561 atctcataac ttccaagttc ctttttatta cttgagttat ggtcttgcat ctgatgtatc
     1621 acttcagata tggcaattat cacaagaaga ttacagaaaa gctgttgatg tatatatgga
     1681 ttttttaaat caaaatactg atgctggatt taaagatgtg gtagaaaagg taaatttaca
     1741 attacctttc caaaatggaa atttagagga aataagctct actttatatg attattttgg
     1801 tatagataat ccacttgaac taaaaaatgc ttcttaattt ctaatacttt atactcattc
     1861 ttaagattta gtattagatt gaaataagtg atattttttc acacacaatt aatgtttaat
     1921 aaaattatat ggtaaagtat cagaaatttt taagactctt gtctttataa tttctgatac
     1981 tttatttatt aataatatta ttattttttt atatatataa ttaaaaggat tgtggaaatc
     2041 aataaaaata tccctgtaaa aatagcacta ttcatatgga acttaataga acttgcatct
     2101 gttaccttat tattcttaat ccagaaccca caaaccatag ttgaaagaaa aagtaaaaca
     2161 gtgataagac ttgaaccaat agttataatt ttcatcttac atcaacatcc tctccaataa
     2221 tattttctat taaaatatca atgaatttat ctcgctcata tttattcttt atatttatac
     2281 cactgtataa ctgaaatgat tctgtagata aagcagaaat ctgtattggt aaaataattt
     2341 gcagggcaat gattcttaga ctcatttcat cttttttatt tccaaaaatt ttacgaagca
     2401 tagggacaat atcaagttct gcttgcatat ctccattggt tatacattga ttcaacacaa
     2461 aaggaagcac tttttcataa tgtaatccag tatcacacaa tttttttaat aaatttttca
     2521 aatcttcaat aggtcttaga gtatatacac tatcgtcata taactctgct attatatttg
     2581 acatcacatc tcctatggca gtactcaaca aattatcctt tgttttaaaa tgataattta
     2641 ttaaaccaac tccaacattt gcacgctcag caatttttct aactgtaatc tcctcaatat
     2701 catctacttc atttaaaaga ttcaatgtaa cttcaattat acgttctcta gcatctttat
     2761 cattactttt cataatagac ctccaaaaca attaacattt tcagttctga acatgttcag
     2821 tataatattt ttttcatcat atgtcaatat aatttaatta taattttagt tgactctaac
     2881 attatgatat ggtttatatt aatattaagc taaaagtacc tttataaagg agacaaaaac
     2941 atgtttaaga taggcgattt ttcaaagcta agtaaaatta gtattcgtat gttacgtcat
     3001 tatgatgaaa taggactttt aactccttct catacgaata aaactaatgg atatagatac
     3061 tatagtgcag accaactttc tactacaaac agaatccatg cactaaaaga tatgggcttt
     3121 ggcttgtatt ctataaaaga aattttgaca gaatataacg ataaagaaag tctcataaaa
     3181 tatctaaata tccaccattc tcaagtaaaa gaacaattag aagatacaca gaaaaaacta
     3241 ttaaaaatag aaaccactat taaaagaatt ggaggaaatg atattatgaa aaattatgat
     3301 gtaactataa agaattttgc accaaaatat atgatgacac ttagaagggt tataccaact
     3361 tatcaagacg aaggaatgtt atggcatcaa gtatttttag agacaaaaga ccagaatgtt
     3421 caaattgaac ccccaaagta ttctaaagca gttttttatg atactggata taaagaagat
     3481 tatgtagatg ttgaagtaca agttgctgta tctggaaagt ataaagatac tgaacatgtt
     3541 aaatttaaga ctgttccaag tgtgactgct gcaactgcaa ttgttaatgg aaatttcaat
     3601 caagttgcag acgcttgtga ggctattggt aattggatat cagataataa ctatgatgta
     3661 gatggtccta tgtttaatat ttatcatgta agtcctggca acgatagtaa tcctgataat
     3721 tgggtaacag aggtatgctt cccagttaaa aagaagtaat ttttatatta attggataat
     3781 attttaattt gttagacaaa ttacttttag tgttatgtat aaatacctac aatttgatta
     3841 ggatattatc ttaatttgtt aaacaaatta cttttagtgt tatgtataaa tatctacaat
     3901 ttaattagga tattatctta atttgtcaga taaatcactt gtgatgttat gtttgaatga
     3961 ctagaattta attaggatat tatcttaatt tgacagataa atcacttgtg atgttatgtt
     4021 taaataccta caatttaatt aggatattat cttaatttga cagataaatc acttgtgatg
     4081 ttgtgtttaa atgactagaa tttgattagg atattatctt aatttgtcag ataaattatt
     4141 tataatgtta tgtttaaata cctacaattt aattaggata ttatctcaat ttgtcagata
     4201 aatcaattat gattttatgt ttaaatgact agaatttgat taggatatta tcttaatttg
     4261 tcagataaat caattataag attatatact atccttatac tctagttaaa tatattttaa
     4321 tcactaatat aaattagttt ttttaacaaa acaataaaag agtaaagtac aaataattcc
     4381 tttgtgctct actcttttta actttcttta gacaatgata taatgataaa tataaaaaat
     4441 tatatattcc attctataga aatatgctca ccaataacat gtatgttgtt aatcaatata
     4501 ttatatcaaa ttatatcaaa attacataaa ccttatagga caatcatatc ttattacaag
     4561 aaaaatgtct tatccatatt gaataagtca ttcagttcat atacaaagtc aatttcattg
     4621 agaaacattt aatatatgtt tataaaaatt tcatactatt ctttcatatt tgcatgtgat
     4681 ataatttatt aactcataat tttgttaatc agttgtgctt gtatattatt tatataatct
     4741 aatattttaa gaaggtggtg taataacttt gaaattaaaa attgttggta gtggcggtat
     4801 gtcaattatt ccaagttcat tttgcaaatg caaaatatgt gaagaagcca gacaaaaagg
     4861 cggaagatat gaacgattag ggccaagttt atatattgat gatataaaaa tgctgataga
     4921 cacacctgaa gatattgcag tagcttgtaa cagacaaaaa atatcggaag taaaacatat
     4981 ttcaatttct caccatgacc cagaccatgt taagggtatt agaattgtcg aaaaaatagg
     5041 atgtgacttt atcacaggag aaaataatcc aattggcttt tatgcgcttc ctgaagtaat
     5101 tgaagatata aaccgaacga atctcgattg tcttaaatat tatggtgatg ttataaattg
     5161 catttctatt aatgaaactt cacatgtaaa gataaataat tttgatattg atttgattaa
     5221 caataatcca gacaggaata taaccttcta tgttatcaaa gagaatgaaa aaaaagtcat
     5281 atatgcctgc tgtaatccca agccatttac tcatagtgat atgtactttg atactgatgt
     5341 tttaataata agtctagttt ctgatgatgg tatattaaat gatggtacaa aacttaaaga
     5401 cacaccattt aaagatgaaa tatttacttt agatgagatt gttgaaatta aaaactatta
     5461 tagaattaaa aagattatca ttacacatat tgacgaaatg tggggaaaat catatggtta
     5521 ttacagtgaa ttagaaaaga aacttgataa catttatttt gcatatgatg gtatggaaat
     5581 tatagtctaa atcattaagt aataagcata atgcaaaata agaaaacttc aatttaacag
     5641 tatagttaaa ctatgtaata tcaacatatc tcagttttta caaaatatca tagtatttat
     5701 ctttataaaa tttcaagtcc tacatatttt aatacttcaa tatctaaata aagtaaaatc
     5761 aaaaatatat tttatataca ttatagacgg cttatcggca ctagataagc cgttttgtca
     5821 caattattat ttgctcaaca aaacttctca taatatataa cagattgaat ctccaactga
     5881 tacttaatta ccgaaactct tattccttaa tatgttgttg cattaaccaa accttcatca
     5941 tcattttttg tggcaatagt tttgctaata aatgactgga ttttgtattt ataccataca
     6001 cagaaatatc cttatttttc tttgcatcct tcaatgcttt tttggcaaca atgtctggag
     6061 acactatacc tgggaaattt tttgtccctt tttcagctac aactatacct ctcttaaata
     6121 gatttgtact catccaacct ggacatacag caattgcatt tatcccttta ctcttaagtt
     6181 caacatttaa tgctcttgta taatttctta taaacgcttt agttgaacta taaatatttt
     6241 gatatggcaa tggctggaat gatgcttgtg aagaaatatt catgatatgg cttccctttt
     6301 gcatatatgg aatacatata agtcccaatg ctacaacagc actaatattt aaatcaatca
     6361 tattaataga ttcatctata cttaaatcat tgtatgaaca gaattttgca aaaccagcat
     6421 tgtttataag gtattttata catacatttt ctttacttaa aaatgtacca atttctttta
     6481 cttcttctat ttttgataaa tcttttgaaa aaatttttac cttacttcca aattcatcta
     6541 ctaattgatt taatctcgcc ttgtttcttg caactgccca tatttctgtt atttcttctt
     6601 catttattaa taactttaga aactctttac caaaaccact attagcaccc gttataattg
     6661 caatcttctt ataactcata attattctcc tttaccataa gtttatacgc tcaaactgta
     6721 aacttatttt ttacaaataa aacaccctct attcagatgt tatattcata ttatttgaga
     6781 tattatattt atatcatttt caataattgt tatatagcat aaaaaatagt gttaatatta
     6841 tagaatcggt attgattata tttaaaaagt agtgttaatt atattataga atcagtattt
     6901 attat
//
LOCUS       DAMJVI010000149         6856 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.154, whole genome shotgun sequence.
ACCESSION   DAMJVI010000149 DAMJVI010000000
VERSION     DAMJVI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.154"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            85..1488
                     /locus_tag="PC545_003397"
     CDS             85..1488
                     /locus_tag="PC545_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2458115.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLALANQNNSSNGTVYFNTIKNNKNDYIYIIYFPLNISKVNMFFDGGK
                     FIGGKSIVFSIIGIIFLIILVSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     ADIYNSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSST
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRFLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDCTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSNTKQKIEGTGLGLAITKQIIEVHGGNIS
                     VESIPGLGSSFYITFPI"
     gene            1707..4184
                     /locus_tag="PC545_003398"
     CDS             1707..4184
                     /locus_tag="PC545_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDF2458116.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMYGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLISADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRNTRGVNALLPKETLSKIINKGSGYTRVLIESEEEITENL
                     VNKLSKELSTEKYMVSNTINETKIISDARQKTMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTRILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPVVISPIGVIISVIVAIIVSSFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSILLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKKILPLYVMNNEISGNGVTLSRLEGTDNIKLYNSM
                     FGINYTNFEIKKQVIEAFNDKRSVILNEDTLKKVGLSIGDTITLSNDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRYLFGDTYNWSRTVE
                     EFNNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            4199..4885
                     /locus_tag="PC545_003399"
     CDS             4199..4885
                     /locus_tag="PC545_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458117.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILDIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLNKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            5159..5992
                     /locus_tag="PC545_003400"
     CDS             5159..5992
                     /locus_tag="PC545_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458118.1"
                     /translation="MLKKESIHFINKNRSLIVCEDNFVYLLPCIQLRNWISNFTISFP
                     NQTIISDNYTIIPHGSVTLVFFYDATGLHSLLFGPTTKPKTVGNIANRCDVILIIEFQ
                     PAGFFPLIGIQQSELIDKVVPFSIINTSLDLEIKKIFNESLSIDKLILKLEELLISNI
                     KIEYSYEFILAIQLIIQNSGNISSQEISKKVFYSSRHLNRLFNQYLGLSMKSFSRLVR
                     INKSIKLLNNNKTSLMSICNELGFYDIPHFIKDFKIVCGITPQKYRANMSDFYSEIAK
                     F"
     gene            6030..6491
                     /locus_tag="PC545_003401"
     CDS             6030..6491
                     /locus_tag="PC545_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HDF2458119.1"
                     /translation="MKFGGVLLIVKDINKSKKFYEDVMEQKITLDLGEHVSFENGFGL
                     QSNYKAIIGYDLPIYQQSNNFQLYFEVNDIKQWESKINRIGNIEFLHYIKEYPWGQRT
                     FRFYDFDKNIIEISESMESVIKRLLKQGLALEEISKRTMYPVEFITQFQQL"
     gene            6522..6770
                     /locus_tag="PC545_003402"
     CDS             6522..6770
                     /locus_tag="PC545_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2458120.1"
                     /translation="MQKTKAWGIIEPEKPESQQQMAHMDFAVNDLEEAVQYAIHCGAT
                     IAEEQFTDDWRVMIDPAGHPFCLCQMKSIMESSHFSLL"
BASE COUNT         2642 a          747 c         1068 g         2399 t
ORIGIN      
        1 aaataaattt ttacatatca ataattattg tctattttat ataagcaata atcaaaataa
       61 aagtttaaga aaagagttga ttaaatgcat aaacctagtt ataaaacagt atttcattca
      121 tacattattt ttttcatgat ttttatttgc ttaatcctga cagtaatagg tattttctta
      181 tcacttatta ctattcagaa accaaatggg gaatttattc gtagtgactg gccgaaaaaa
      241 tttacagaaa actttaatga acaaatcgta tttatagata ataagccaca agtaaaacag
      301 tctggattag aaaaacttcg agaaaatcaa ttgtggttgc aaattttaga ttctagtgga
      361 aatagagttt atgggttttt agaaccaaaa gagcataaag attattattc taattctgaa
      421 ttattagcat tagctaacca aaataattca agtaatggaa ctgtatactt taatacaatt
      481 aaaaataata aaaatgacta tatttatatt atatattttc cattaaatat atctaaagtg
      541 aatatgtttt ttgatggagg taaatttata ggtggtaagt caattgtatt ttcaatcata
      601 gggattatat ttttaataat tttggtttct ggaattgctt atggttactg ggtaacaaag
      661 tgcatatcaa atattacatc tgctgttagc gatatttcaa aacgaagcta tcttccagtt
      721 aatattaaag gtgtttttgc tgatatatat aatagtttaa atattttaaa tgaagaaatt
      781 catagaagtg atgagataca aaataaaact gatattatga gagaagagtg gatttcaaat
      841 ataactcatg atttaaaaac tccactttca ccaataaaag ggtatgcaga gcttcttaca
      901 gatagtagta ctaattattc acctgaaaaa gtatataaat atgcttcaat catgttaaag
      961 aatatagaat atacaaatac acttataaac gatttaaaat taacctatca attaaaaaat
     1021 ggaataatcc ctttaaataa aaataattat gatttagtaa gatttttgaa agaattatcc
     1081 attgatattt taaactatcc agaatatgaa tcaaatatag ttgaatttaa gagtaatata
     1141 gataatatta actttgattt tgattgtaca ctattaaaac gagcttttag taatcttatt
     1201 gtcaatgctt ttacatatgg agataaaaat actaaagttt gtgtacatat aacattaaat
     1261 gaaagaattg aaatcattat atctgataat ggaaaaggga tgtcaagaga agaagcatca
     1321 aatttgttta atcgttatta tagaggaagc aatacaaaac aaaaaataga aggaactggt
     1381 ttaggactag caataacgaa gcaaattatt gaagttcatg gagggaatat atctgttgaa
     1441 agtatacctg gattaggtag ctcattttat atcacctttc ctatttaatt aaggttaatt
     1501 aaggtcaaat aaaaaatata ttaaggttga tagattatta tatttatata gtagtttatc
     1561 aatctttttt atttaagggg tgatttgatt aaaaatatta taaatagaat gataaaagta
     1621 tggaggagtc tattagagat ttaatttaga ttaaagtata aaaatattag attgaaatat
     1681 cttagataag gaaagaaggt gtaaatttga aaattatatt aaagtatatt ttcacaaata
     1741 ttaaagaacg aaaaattaga acagcagtta tgttattgtc catagtttta tcaacagttt
     1801 tactttttgt ttcattctca attggattat cttatgaaag tgcacaaaga aaaatggcaa
     1861 aaggtatgta tggtacagcg actatatctg tccaaagtaa aaatccagat atattaacca
     1921 atttagaaga catacctgat ttaaatgcta taaaatcaaa ggttggtgta ttggaaagct
     1981 ctgctattta taataaaggg ggatactatg aagaatttag tcttatatca gcagacttaa
     2041 gtcaactaaa taaaatcaac aagccacgat tagaaaatgg cgatagtatt actgattttt
     2101 ctggtgataa aattattctt ccaaatcgct ttacatcaaa atataaaata aaaaaaggtg
     2161 attctattac tttgcagatt tatggaaagt cttatacttt tcaagttagt gatattgcat
     2221 cttatgatac agtatttttg aggaacacaa gaggtgtaaa tgcactgcta cccaaagaga
     2281 ctctatcaaa aattataaat aaaggcagtg gatatacaag agttcttata gagtcagaag
     2341 aagaaattac agaaaatcta gtaaataaat tatccaaaga actttctact gaaaaatata
     2401 tggtatcaaa tactataaat gaaactaaga taatttcaga tgctagacaa aagacaatgc
     2461 ctttcttttt aataagtttt tttgcattaa cccttagtat atttataata tacagcagtt
     2521 ataaggttat tacattagaa cgtcttcctt ttattggaac atttagaagt attggtgcca
     2581 atgaaaaaac tgtaactcgt atattgatgc ttgaaagtat attatacgga agtataggag
     2641 gattaattgc tattcctatt ggtgtagttg ttttaaattt aatgcttcat gggttaggaa
     2701 attctctcga acaaggaata tcaattccag tagtaatatc acccattgga gtgattatat
     2761 ctgttatagt agcaataatc gtttcttcat ttagtgcata tatacctgtt aaaaaagcta
     2821 gccatttgcc gataaaaaat attgtactag gaacagtaga agaaaagaat gtttcaaatc
     2881 gttctatact ttttatagga agtataatgt ttattttatc tattttactt ccaagaatat
     2941 cccctgaaaa tacattgtat ctagctggtg gattttcatt actgggatta attgtagcta
     3001 ctattgtgtt gattcctctt attactgata tcatgtctat agtttttgag tttgtttata
     3061 aaaatatttt agggaatgag ggtaaattag ctgctagaaa tatgaaaaat aacaaaaata
     3121 taatacagaa tatcacttta ctatttataa gtatttctgc agttattgca ataagtgttg
     3181 ttggtaactt tgtcaaaact tatataacag atgtgtttcg tgacgcagaa ttacaaggtt
     3241 ttgcagatgg aaagatgaat gaagagttta ttgaagatgt tagacatatg gatggaatta
     3301 aaaaaatatt acctctttat gttatgaata atgagatttc tggtaatggt gtaacattat
     3361 caagactaga gggtacagat aatataaaac tatataactc tatgtttgga atcaattata
     3421 caaattttga gataaaaaaa caagtaatag aagcttttaa tgataaacgt tctgttattt
     3481 tgaatgaaga tactctaaaa aaagtaggct tatctatagg agatacaata actctatcta
     3541 atgataagta tgatttttct tataaaatag taggtagttt taaatcccgc gcaaatgatg
     3601 tagaagctgt tataccatca cattatgcag ttaatgattt tgataaaact aattatggtt
     3661 ttttagtata tacagcagtc aatccagatg ctgttatgat tcagataaga tatttatttg
     3721 gagatacata taattggagt cgtacagttg aagaatttaa taatgattct ttaaatacta
     3781 taagtagttt tttgtctcct atgaataaga tgacctattt catttttctt cttgctacag
     3841 taggtattat taataatcta ctgattaatt atattcaaaa aagacgttct atagctatgt
     3901 ataaatctat aggactaagt aataaacaaa atataaaagt gacattgatt gaagggttta
     3961 cttcaggttt acttggagct gttattggta ttgtgatatc tatacttgag attcaaacta
     4021 tctttatagt tgcaggacca aaaatttcaa tgaaaccaga tttagatttt aaaacattta
     4081 ttattgttgg gttattggga attatagtaa ctttaatagg ttcgatagtg cctattataa
     4141 aagggaagaa aatgaaactg atagaagaaa ttaagtttga ataattatgg aggttaatat
     4201 gaaaattaca cataatagtt tagctgtaga agcaaaaaat attatcaaag aatataaaat
     4261 tggaaataca acaactcgag tgttaaaaga ggtatcttta caagtaatga aaggtgaatt
     4321 tgtttctatc atgggtcaat ctggttctgg caaaagtact cttttatata ttttaggtgg
     4381 tttagatact ccaacgagcg gaaaagtgta tatgaatgga gctgatatat cacattttaa
     4441 tgatgagaaa atgagcataa tacgtcgtag aaatatagga ttcgtatttc aattttataa
     4501 cttaatacca aatttaaatg tggaggaaaa tataatgctt cctttgttat tagatggtaa
     4561 aaatttaaaa gattataaaa atcaattaga tgaaatctta gatatagttg gtttaactga
     4621 tagacgtaaa catacaccac gagagttatc tggtggtcag caacaacgtg ttgctatagc
     4681 tcgagcttta attggaaaac cagagattct atttgctgat gagcctactg gaaatttaga
     4741 tagtaaaaca gggatagaaa taatagattt actgaataaa ataaacagag acaatggaca
     4801 aactataatt atggtaactc actcaccaga agcagccaaa agtagtagtc gtactattac
     4861 agtttcagat ggtttaattg tataaggact aattattgta tttgagataa gcatttttaa
     4921 ttatataaat tagacatatg taaagtggat ataagttaaa tactataaat gtgctataat
     4981 attaaaataa cagataaaag tactatattt tttattgatt attttaattt tatttgttta
     5041 ttaaatatct aatgagtatt catactatct ctattattaa aagcagttaa tgatatatag
     5101 agataatatg gattcttctt actttaataa atattcacga taggacagga tataatatat
     5161 gttaaaaaaa gaaagtattc attttataaa taaaaatagg tcacttatag tttgtgaaga
     5221 caattttgtt tatctcttgc cttgtataca attaagaaat tggatatcga attttactat
     5281 atcgtttcca aatcaaacaa taatttctga taattacacg attattcctc atggaagtgt
     5341 aactttggtg tttttttatg atgccacagg gttacatagt ctattatttg gtccaactac
     5401 taaaccaaaa acagtaggaa atattgctaa tagatgtgat gttatactaa ttatagaatt
     5461 tcaacctgct ggttttttcc cgcttatagg tatacagcaa agtgaactta ttgataaagt
     5521 tgttccattt tctataatta atacttcatt agatttagaa attaaaaaaa tatttaatga
     5581 atctttatct atagataaat tgattttaaa gttggaagaa ttattgattt ctaatattaa
     5641 aattgaatac tcatatgagt ttattttggc aattcagttg attattcaaa actctggaaa
     5701 tatatcttca caagaaattt ctaaaaaagt attttatagt tctaggcatt taaacagact
     5761 ctttaatcag tacctagggt tgagtatgaa gtcattttct cgtttggtac gtattaataa
     5821 atctataaaa cttctaaaca ataataaaac ttcattgatg tctatatgta atgaattagg
     5881 attctatgat ataccacatt ttattaaaga ttttaaaatt gtctgtggaa taactcctca
     5941 aaaataccga gctaatatgt ctgattttta cagtgaaatt gcaaaatttt aagttataat
     6001 acttataaca aagaaaggat agtgataaga tgaaatttgg aggagtattg ctaattgtca
     6061 aagacataaa taaatcaaaa aagttttatg aagatgttat ggagcaaaaa atcacattag
     6121 atttaggaga acatgtatca tttgaaaatg gatttggatt acaaagtaat tataaagcta
     6181 taattggata tgatttacca atatatcaac aatcaaataa ctttcagttg tattttgagg
     6241 ttaatgacat caaacaatgg gaaagtaaga taaatagaat aggaaatatt gaatttttgc
     6301 attatataaa agaataccca tggggtcaaa gaacttttag gttttatgat tttgataaaa
     6361 atattataga aatttcagaa agtatggaaa gcgtcataaa gaggctttta aaacaaggtt
     6421 tagctttaga agaaatttca aaacgcacta tgtatccagt tgaatttatt acacaatttc
     6481 aacaattata aaatattaaa atatataatt ttttctttat attgcaaaaa acaaaggctt
     6541 ggggcataat agaaccagaa aaacctgaat ctcaacagca aatggctcat atggatttcg
     6601 ctgttaatga tttagaagaa gcagttcaat atgcaattca ttgtggagct acaatcgcag
     6661 aggagcaatt tactgatgac tggagagtta tgattgaccc agctggacac cctttttgct
     6721 tatgtcagat gaaatcaatt atggaaagta gccatttttc attgttatag accaaaatat
     6781 tgtttattaa ttacaaaaaa gcacaacagt atataccgtt gtgcttttac ttattataca
     6841 tgaatacact aaaaaa
//
LOCUS       DAMJVI010000150         6792 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.155, whole genome shotgun sequence.
ACCESSION   DAMJVI010000150 DAMJVI010000000
VERSION     DAMJVI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.155"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..138)
                     /locus_tag="PC545_003403"
     CDS             complement(<1..138)
                     /locus_tag="PC545_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458121.1"
                     /translation="MIAVKKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KN"
     gene            complement(786..1193)
                     /locus_tag="PC545_003404"
     CDS             complement(786..1193)
                     /locus_tag="PC545_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458122.1"
                     /translation="MSKVAKATFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDISSKQGEKQALKFLNNVLNIIVGICIVVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTKILIKK"
     gene            complement(1667..2551)
                     /locus_tag="PC545_003405"
     CDS             complement(1667..2551)
                     /locus_tag="PC545_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2458123.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVIHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGKVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
     gene            complement(2841..3317)
                     /locus_tag="PC545_003406"
     CDS             complement(2841..3317)
                     /locus_tag="PC545_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HDF2458124.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
     gene            3583..4758
                     /locus_tag="PC545_003407"
     CDS             3583..4758
                     /locus_tag="PC545_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HDF2458125.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQNYDYNSMKNTLDNVSDNVNEQLKKLGESVKGSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     NDSDTNSSKNDTSSNNENNDSSQTNENNTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            complement(4839..5642)
                     /gene="proC"
                     /locus_tag="PC545_003408"
     CDS             complement(4839..5642)
                     /gene="proC"
                     /locus_tag="PC545_003408"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2458126.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQGTLDRI
                     DANFGINTTKDSKEVTRTSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIIGEDKKIVRTMPNTPALVNEAMSSLSINKNINKEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(5682..6266)
                     /locus_tag="PC545_003409"
     CDS             complement(5682..6266)
                     /locus_tag="PC545_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458127.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVVKILVVSIPNPFEKSDAVKEA"
BASE COUNT         2233 a         1145 c          756 g         2658 t
ORIGIN      
        1 gtttttataa acatctattc ttttagcaag atacttatta tatacaaatc tacaacatcc
       61 aaaagttttg ttaattaact cttgttgttt tttatttgga tacattctaa acttataagc
      121 ttttttcact gctatcacct cactttaatt gttttttgat acacttttta atgattttat
      181 cacattttat cacatttaat atattatata atgctgtggt aaacaattta ttaatcacct
      241 ataataatgt taacaaatgt aattatttaa aacaattatt tagaaggttt tatatttaat
      301 tttaaataac atatttaaga accgtaagtc ttaactttta tcattttgag gttgtcgttc
      361 acataagttc gctactacct atgcagttct cttatgaact tcttacattt tcatgtaagc
      421 acagactata tcttatcctt cagcattatc cgttaaggtc tacccacttc cactaccaat
      481 agcttgtagt gtacttccct caagaggaat agtcgttgaa gtttctcctg ttcggagttt
      541 acctgctgat tgcccatttt aaagtactta ggatttaacc ttataccatc taaccaattt
      601 tttctacttt cgtaactttc acacttaggt ttatttcatc cttatgtttt agtttgatta
      661 gctttagggg tttccagcaa ttcaagtagt attggatagg ttctatccta tctctacata
      721 taggtttccc tatatgctga ctattttagc tactaactca tgactaaagt cacgagtgtg
      781 tgtagctatt ttttaatcaa tattttagta aattttacgg taagtaaaaa cctctctcct
      841 tcaaatccta tagcaaatat acttacaagt tgctttgaga aaataactcc taatatagct
      901 acaactatgc atataccaac tattatattt aaaacattgt ttaaaaactt tagtgcttgc
      961 ttttcacctt gtttactact tatatcttga tacattggaa taaaagtagt aactatagca
     1021 gtacctattg ctgcaaatat aatatttggt atattcatag ctgttaaata actctctgta
     1081 tataagcctg taccataaat tgatgataat actaattctc tacccattcc aagaatctta
     1141 gagattatag taactatcat caaataaaaa gttgctttag ctaccttgct cattttatca
     1201 ctccacttct aacatacctc tttttaatca agtaaaattt agctcattag atataattct
     1261 atcaactaat ttgccttgct tagccctatc aaaatataat taacacctat ttcttaatat
     1321 atcacaaact acattattct tcatattttt ttatttctcc aataagtttt acatttttcc
     1381 tatataaatt gtatagcttc aattatattc aaagaaatgg agttgctacc cgataacttt
     1441 aaaagtaaaa aacagatttt attatctttt taaactatat tctaacatat tttgtccata
     1501 ttgagaaatt ttactaaact attatgtact ttattaaata aataaaataa cagcacacct
     1561 ataaaataca agtgtgctgt tcataaacag attttataaa aaaataattc ctaagtagag
     1621 attaacatgc tttctcatac tctcatatat aatgtgtcta aaaatattaa taaaaatgtt
     1681 ttaattgttt taacttactt ggatgtctta attttcttat tgctttagct tctatctgtc
     1741 ttattcgttc tctggtaact ccaaatacct ttccaatctg ttctagtgtt ttagggtcat
     1801 catcatcaat tccaaatctc attcttaaaa cttgctgttc ttgctctcca agacctgtaa
     1861 gtagttcttc tatcttttct tttaaattat gctgttcaac ttcgtgtatt acaacatctt
     1921 taaaatcagc atctgatggt ataaattcaa ctaaactact atcctcatct tcttttaatg
     1981 gagtatccaa agatacaaca tttttatctc ttcttaataa ttccaacact ttgtcaactt
     2041 ctaagccaca tgcatctgca atctcatcta tagttggctc tcttaataat acatttaaaa
     2101 gttcttgttt ctttttcttt acaaaattta ttctctgatg aagatgtata ggtatcctaa
     2161 tagtattttc gcaatcgtcg atatatcttg ttatactttg ttttatccac caagttgcat
     2221 aagtgctaaa tttgtatcct tttttataat cgtacttttc tacagctttt ataagtccta
     2281 tatttcctgc ttgaattaaa tccaacatat caatactatc tcttttatat ctctttgcta
     2341 tgctcactac caacctataa tttgaattta taaatttttc ttttgctact gaagatgagt
     2401 tgattatttc cttagccagc tctacctctt ctcctgctga caacattttg tattcctcta
     2461 tttcctttaa atacattttc atcgcattcg atacatttga tgaacccaca caaatataat
     2521 tttcataatt attttctttt ttagtatcca taatcaaacc ccttctattc atagaatttg
     2581 ctaaaacaaa aataaataat ttaaacctac ataatctaca attacataat atcccattct
     2641 ttaaatgaat tgttttgtat attatgtata caaatttata aaaactatac ctataatatt
     2701 attctacttc taacagcaat atcctcctta tatatataat ttattttatt atatgttgtt
     2761 atattcttag ttttttgaat gatatatgtc taaaaaaaga ctgtttacta aacagtcttt
     2821 tttatatata tgctcaatat ttaattttca tgatgatgat tacatccaca tccacaattt
     2881 tcatcatggt gatgatgttc ttctaaatct tttacctgtt gttccatttc atgaaactct
     2941 tttaccatta tatcataagt tggaagagct gcctcagtat cgtattcttc ccctctccaa
     3001 tcagcgtaca tcatagcatc tccattagtt aaaaccaatc tacctgacat agttatatta
     3061 tccacttctt ctaaaaattc tatttgtttt tgtaaaactt ctccttctaa ctccacatct
     3121 tcatctaaac attgcactat cattacaggt gcataataat cttcttcatc tttaacattt
     3181 acaacaatat ctaaaattcc ttctgcacct tcttcaaatt ctgccaactc aacatttgta
     3241 tcaatcatat cttgaggtac taataaatct tctattaaat attttattac tctatctttt
     3301 actaattctt ttgacattta tttttctcct tccgattcta taacattatc ataatcattt
     3361 ctatattata gtatactaca ttaattgttt tttcaatctc ttacaaaaat tattttttat
     3421 tatcttcctt atattccctt ttttattaca aagttgtaat cgcattgatt taaaaaatag
     3481 tttatattta gtataataga aatgtagcac atatttttat tcaatatatt aataaaaaaa
     3541 tatattgaaa atgtaatgta tgttaaatct aggaggaata ttatgaagtt taaaaaacaa
     3601 attagttcta ttgttgtgtc tactatgctt gtacttggga gtatgaacct ttcttatgca
     3661 gacggtacaa atgtagttac acttggagct aacttatcag agtctcaaaa gcagcaaatg
     3721 cttaattact ttggagtaaa aaaagaccaa gtcttagttt tagatgtaac aaatgcagaa
     3781 gaaagacagt atttacaagg tgttgcaaca gagggtcaac ttggaaaagt tactatctct
     3841 tgctcatacg ttgaacctac taaaaaagga aatggaatta atgttaaaac agcaaactta
     3901 acttgggtaa ctagttcaat gattgcttca acccttacaa cagctggtct tgaagatgca
     3961 aatgttattg ctgctgctcc atatcctgta agtggtactg gagcattgac aggaatcatg
     4021 aaagcatttg aggatgcttc aggaaagaaa cttgatgaaa ctaagaaaga aattgcttct
     4081 gaagaattgg tagttacagg tgacttagga gatgatatag ggcaagaaaa agcaacaggt
     4141 gtaatgaatg atataaaaac agaaattgta aaaaatggta caaaagatac caatcaaata
     4201 gcaaatacaa taaataatgt tgtaaataat tacaatatta ctattacacc agaacaaaaa
     4261 gaacaaatta aaggtgtaat gaaaaaaata gctgaccaaa attacgatta caatagcatg
     4321 aaaaatacac tagataatgt atcagataat gtaaatgaac aattaaagaa attaggtgaa
     4381 agtgttaagg gttctggtat actagatact attggtggat ggtttagtgg aataggagac
     4441 tggttctctg gtttattctc aggcggagat aaaaaagatt tgggaatctt aaataatact
     4501 aatgatgagt ctcttggctc tgatgctgta ataaattcta cagaaggtgt tcaaataaat
     4561 ccacaaaata atactaattc taacaatgat agtgatacaa atagctcaaa aaatgatact
     4621 tcaagtaata atgaaaataa cgatagttct caaacaaatg aaaacaacac aacaaataat
     4681 tcagaaaatc aatcaaataa taatgactca gttggtttct ttgaaaaaat aaaaaattgg
     4741 ctttcaggct tattttagaa attaaacaaa taaaaagaat tgcctaaaat ggaaattttc
     4801 tataaaaggc aattctcttt atttacaata aatttttatt actcactcat ttttttagat
     4861 ttttctatac atgcacatat tgcttttatc acagaagctc taaaaccttc ttcttcaagt
     4921 actttaactg cttctatagt agttccacca ggtgaacata ccatatcttt taattcacca
     4981 ggatgttttc ctgtctcaag taccattttt gcagaaccca taacagcctg tgatgcaaac
     5041 ttatatgctt gtggtcttgg cattcctgca attactgctg catctgctat agcttctata
     5101 aataaaaata catatgctgg tgcagaacca cttgcaccaa ttacagcttc tataagatac
     5161 tctggtacta cctcagtatt tccaaaagaa ttaaatactt ctgtaactgc ttctaagtct
     5221 tctttgttta tatttttatt tatagaaaga gaagacatgg cttcatttac aagagctgga
     5281 gtatttggca tagttctaac tattttttta tcttctccta taatagactc tatatctttt
     5341 atagattttc ctgctgctat tgtaactata attttattgt catctataaa gtcttttatc
     5401 tcttccaaaa tatcatcata tatatctggt ttaacagcta caattactat atcagaggtt
     5461 ctagttactt ccttcgagtc ttttgtagta tttattccaa aatttgcatc tattctatca
     5521 agagttcctt gactatatgc tgatgctata accatattag gttcaactaa ttttgaattt
     5581 actattcccc ctatcatagc gcttgccata tttcctgcac ctatgaatcc aatttttttc
     5641 actttagtac ctccttattg actaatagta atcgttaaaa tttaagcttc tttaactgca
     5701 tcagactttt caaatggatt tggtatactt actaccaaaa ttttcactac cttattagta
     5761 tagttgaccc aattatgatt gttcttttca ctattatagt gtattgtgtc tcctggatac
     5821 atttctattt gttcatctcc caaaaacact gtaagagtac cctctaatac atacacaaat
     5881 tcctcacctt catgtacgta acaacaaatt tcctcactac tcttacttgg aagaatttca
     5941 attaatcttg gtagcattgt tttttccttt aaactagatg ataaatgata atgtataaat
     6001 gttgagtttt ctacatcaaa tacttctttt tcataacttc ttaatacagc tcttttatgc
     6061 tcttttggct tcataaaaaa gtaagttaat tctacatcta gtacacttgc tatttttcct
     6121 aaggaatctg ttgccacact tgttaatcct ctttcaagtt gtgataaaaa accaattgac
     6181 aagtttgttt tttcactcat atccttcagc gtcatttgct tttgagttct gagttgcttt
     6241 atttttgctc ctatgtcctt attcaaaacc atcaccccta aataatttta attttttcat
     6301 aatataaata caaaaatata aatataggta ctgaacctat ctttatttca tttaaaaaaa
     6361 tttttataag tattactata atttatcaat ctttttttgt ctacattttt acgattattt
     6421 acgatttatt tttcacatat gtatatttaa tcttgccaac taaagataca ttttttataa
     6481 gcatttactc tttatataat aaaacttaag ttgttcatga gtcttcttct ggtttaatta
     6541 ttaaaatttc aaattatatt attatttcta cacattatta catcctttag tacaattata
     6601 acataatttt acaatttaat gatatctatt caatgtcttt ttttactcta caacatattt
     6661 tattttagag tttaatacta tatatatttt ttatatacag ccttgtcatt ttttagattg
     6721 tcaagagtta ttgaagtatt ttatgtagat ttagatttta ttttctaatt tctcatttaa
     6781 caagaattgc tt
//
LOCUS       DAMJVI010000151         6726 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.156, whole genome shotgun sequence.
ACCESSION   DAMJVI010000151 DAMJVI010000000
VERSION     DAMJVI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.156"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            218..1051
                     /locus_tag="PC545_003410"
     CDS             218..1051
                     /locus_tag="PC545_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HDF2458128.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFIVVGVRVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTSIEVSKGPSKSSGKIAYITIDDGPSKFTDEMIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIVQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
     gene            1144..2076
                     /locus_tag="PC545_003411"
     CDS             1144..2076
                     /locus_tag="PC545_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2458129.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKDYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEVSASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFTKDDKNAEFTKEVFKLKKNEVSNVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            2128..2610
                     /locus_tag="PC545_003412"
     CDS             2128..2610
                     /locus_tag="PC545_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2458130.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILVRTSIDFIRKNRKYNNMTDIDLIDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            2622..3491
                     /locus_tag="PC545_003413"
     CDS             2622..3491
                     /locus_tag="PC545_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2458131.1"
                     /translation="MTSKERLNKLKDEYHKIPIPKKLDTIINHEKIENIYREKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFQERMEKIQSSKNKGYKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKKDSTKSYFIDQKKDLPVEDFKTINKYQDFYFNKNEDLVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            3534..4289
                     /locus_tag="PC545_003414"
     CDS             3534..4289
                     /locus_tag="PC545_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDF2458132.1"
                     /translation="MTTYKKLKKSIFKIAIFSICLLVSLVDTNPIFAGSSFNETSNSD
                     TIKVLNAKSINFKQDNYEVNVSIPKIKGLKDKTLENKLNNEFLQDGKKIYSEFQNKMK
                     KENNKGHRYLNVIHNVKNNSKDFLSIEMTKEEIEASSSITKKHYTVDKNRGILLNLPI
                     FFKDSRYIRAISDNIKEQMRNQIKKDDTKSYFIDQDKDSPVKDFESINKYQDFYFNKN
                     GNLVISFDEYEVAPGYMGAVEFVIPNKVIKRLR"
     gene            4331..5152
                     /locus_tag="PC545_003415"
     CDS             4331..5152
                     /locus_tag="PC545_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory factor"
                     /protein_id="HDF2458133.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVVVLDSFENIPDFPTDENSYIIILTRGHLYDSSALEWALKREAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSKVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            5157..5918
                     /locus_tag="PC545_003416"
     CDS             5157..5918
                     /locus_tag="PC545_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDF2458134.1"
                     /translation="MNTGSKDIDTLKVNFRGIEIKAVLVYKNRKNISIKIDPFGKITV
                     MSPPKISKKIIKDIIIEKGDWILKKSEKCKGREEVYKQRMFITGEKFLYLGEEYHLVI
                     KELLRDSVKKSNCRITINEYQIVVQTNDTSTDFIKKSLKSWYKMESERIVLERIDFLK
                     LNCEIMKQLVPASVKVKEQNKRWGSCTAQKNIYINSKISMARLDVIDYVIIHEFSHLA
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKIII"
     misc_binding    6079..6336
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6449..6688
                     /locus_tag="PC545_003417"
     CDS             6449..6688
                     /locus_tag="PC545_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HDF2458135.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMRH"
BASE COUNT         2960 a          631 c         1144 g         1991 t
ORIGIN      
        1 attaattgta aaaaacacta tataatactc aattttcaac attctataca attatacaga
       61 aattatacag cataataatc accgaaataa caaattttaa aaattaagca aaactttttg
      121 tcgaagttca acgtctatat atatgtagtg taaatttttg ggataaaata ctacaaaaat
      181 ataaatgtaa aaatatagta atattggagg tcgaacgatg agaaataaaa agaaaaaaat
      241 aaatagaaag aagttgtatt tgcttttaag tgctattgta gtgtgtctgg cttttatagt
      301 agttggtgtt cgcgtcagct ctttaaatat gaaggaaaat gaaattagta gaaatgcaaa
      361 actgtcaagt tctactatta cagatatagt atctaataca tcaatagaag ttagtaaagg
      421 gccaagtaaa agttctggaa aaatagcata cataactata gatgatggtc catctaaatt
      481 tactgacgag atgataaaaa ctttaaataa atacaatgtt aaagcaactt tttttatgat
      541 agatgggaat atgaaggaat atccacagca agttaaaaat ataattaaaa atgggaacac
      601 agcaggtttt catagtgtat cacatgatat tcacaagctt tatgttacta gtacatcagc
      661 aaaagaggaa tttgatacaa atgatcaaac tttttataag ataactggta aacattcaaa
      721 agtaataaga ataccttatg gaagcaaacc atatacacca caagcctctt atcaggcttt
      781 agttgatgct ggatataaaa tttgggactg ggacttagat acagaagact ggagatctaa
      841 ttcttctcaa attgtacaaa atgtaaaaaa tcatattaag aatagaaaag gtgaagataa
      901 agaccaactt gtagttttaa tgcatgagaa aaaacagagt gcagaagctt tagattcagt
      961 tttaaaattc ttatcagatg aaggttatga atttgcgcca gtagatcaaa accaaatacc
     1021 aaaaaattat tggttacgta atttagagta gttaaaatat aagaatattg attgatattt
     1081 ttagaaattg agaataaaaa tgagtgaggt gtaggtatat tgaataaaaa actttatata
     1141 ggaatggtcg gaatattgtc tttgatgatg gttggttgta ataaaagttt agcaaaagta
     1201 aatgatgtgg aaatcacaaa agaacagtac aaaaaaacaa aagcagtttt atctgctact
     1261 aataattata taaatgggca gtctttagat gaattagaaa aaactttgga taaaaaaggt
     1321 agaaataaat tagaaaatgt tataatatcg tttatggtgg ataatgaact tttatatcaa
     1381 gaggctaagg ataaaggact aactccaagt aaaagtgaag tagactcaaa atatcaagaa
     1441 ctagaagata agatgaattt gaatacaagc tataaggaaa aaatggacaa agcaggtgtt
     1501 gataaagatt atttaaaaca agaaatatct agagatttag caatagataa aaataaaaag
     1561 gcctttgaag atagaataaa cataagtgat aatgatatgg aagcctacta tacaagtcat
     1621 aaaaaagatt ttaatgtaga agaagtaagt gcttctcaga tacttatatc gactttagat
     1681 aaaaataaaa aagaagtaag caaagataaa aaagaagctt tgaagaagaa ggcagataat
     1741 atattgacta aaatcaaaaa tggagaaagt tttgaaagtt tggctaaaaa atattcggat
     1801 gataaagcaa ctggaaaaaa tggaggacag ctaggatatt ttacaaagga tgataaaaat
     1861 gctgagttta caaaggaagt gttcaaatta aagaaaaatg aagtttcaaa tgtgtttgaa
     1921 actagttatg gatatcatat agtcaaagtt actgataaaa gagaaagaca aaaaagtttt
     1981 aatgagtgtc agagtttaat aagagaatct attttaaatg aaaagtatat tgagcatata
     2041 aaaaaactga atgaagatgc taaaatagac agatagaatt tctaacagct caatcctagc
     2101 ctgtaatcgg agggagattt tacaggaatg tttaagagtt taaagagaac gaaagttgag
     2161 aaatacatta tcgataataa agactctttt tatagaatag catatagtta tacgaagaat
     2221 gaagaagatg ccttagatgt ggttcaagaa gccatgtata aggcactata ctcagtggaa
     2281 aatattaaag aagtaaatta tataaagaca tggttttata aaattttagt tagaacgagt
     2341 atagatttta ttagaaaaaa ccgtaaatat aataatatga cagatattga ccttatagat
     2401 gagactggag agtatgataa atatactgat ttggatttaa gaagagcttt ggaagaatta
     2461 cctattgaat acaaatctat tataatatta agattttttg aagatttaaa gattgaagaa
     2521 gtagcaataa tattggatga aaatgtgaat actgttaaaa ctaggttata tactgcttta
     2581 aaaaaactaa aattaaaaat tgaagagtag ggataaaaag aatgacaagc aaagaaaggt
     2641 taaataagtt aaaagatgag tatcataaaa ttccaatacc caagaaatta gatactataa
     2701 taaatcatga aaaaatagaa aacatatatc gagagaaaaa taaaaaagta aatagattaa
     2761 gatttaaagt tgcaatagcc tttgcatgta tatttacagt attagtaaat ataagtcctg
     2821 tatttgcaga caatttttct aagatacctg taattggagc aatagtagaa gtaataacta
     2881 taaaaaatta tagtttaaaa agtgaaaatt atgaagctga gattgatatt cctaaaatac
     2941 gaggtttaaa agataaaaac ttagaacaaa gattaaacag tagttttatg gaagatggaa
     3001 aaaggcttta tcatcaattc caagagagaa tggagaagat acaatcatca aagaataaag
     3061 gatataaatc tttaagtttg agttatagtg taaaaaataa ttctaaaaaa tttctatcaa
     3121 tagagatgac aaaaaatgag atagaggctt catcatatgt gagtaaggta cattatacta
     3181 ttgataaaaa aagacaaata gttttgactt tgccaatgct ttttaaagat gataagtata
     3241 taaaagttat aagtgataat ataaaagaac aaatgagaga acaaatgaaa aaggatagta
     3301 caaaatccta ttttatagat caaaaaaaag acttgcctgt tgaagatttt aaaacaatta
     3361 ataagtatca ggatttttat tttaataaaa atgaagacct tgtaatttgt tttgatgaat
     3421 atgaagtagc acctggttat atgggcgcag ttgagtttgt aataccatat aaagtaataa
     3481 aagatttgta aattgaatag tagtttatat ataaatcttt taaggaggac aatatgacta
     3541 catataaaaa acttaaaaaa tctatattta aaattgctat attttcaata tgcttattag
     3601 tttcattagt agatacaaac cctatatttg caggtagttc ctttaatgaa acttcaaata
     3661 gtgatacgat aaaggtgtta aatgctaaga gtatcaactt taaacaagac aattatgaag
     3721 tgaatgtgag tattcctaaa ataaaaggtt taaaagataa aactttagag aataaattaa
     3781 acaatgagtt tttgcaagat gggaaaaaaa tttacagtga atttcaaaat aaaatgaaaa
     3841 aggaaaataa taagggtcat agatacctaa atgtaatcca taatgtcaaa aataattcta
     3901 aggacttttt atcaatagaa atgacaaaag aagagataga agcttcctca tctataacta
     3961 agaagcatta cactgttgat aaaaatagag gaatactatt aaatttacca atatttttta
     4021 aagatagtag atatattaga gctataagtg ataatataaa agagcagatg agaaatcaaa
     4081 ttaagaaaga tgatacaaaa tcttatttta tagaccaaga taaagactcg ccagtgaaag
     4141 attttgaatc tataaataag tatcaagatt tttattttaa taaaaatgga aatcttgtga
     4201 ttagttttga tgaatatgaa gtagctcctg gttatatggg agcagtagaa tttgtgatac
     4261 caaataaagt aataaaaaga ctaagataga tatatagaag atttttagat agactggtaa
     4321 ggagaattat atgaatatat atgaacaagc aataagatta atagaaaata atgaggattt
     4381 cgcatttgct acaataacgt ctcattcagg ttcaacacca cgtgaaactg gagcaatgat
     4441 gattgtgaaa aatgattcta ctatatttgg ctcagtagga ggaggtagtg tagaagcaga
     4501 atgtataaaa catgcaatta atgttataaa aaacagagaa tcaatgttgt ataaatttac
     4561 tctaaacaaa tctgatgtgg caaaactagg aatgatatgt ggaggtactg gagaaataca
     4621 aatagatttt atagatagta aattaaaaag taatatagaa aaatttaata agagactaaa
     4681 ggaaaataca agtaaagcat atatatttgg tgcaggtcat atttcaagag atgttgctgt
     4741 gatactatct cttttagaat ttagaacagt agtaatagat gatagagaag aatttgctaa
     4801 tcatgaaagg tttccagaat cagaagtagt agttttagat tcttttgaaa acataccaga
     4861 ttttcccact gacgaaaata gttatataat tattttaaca agaggtcatc tatatgattc
     4921 aagtgcttta gaatgggctc ttaaaagaga ggctggttat ataggtatga ttggaagtag
     4981 aacaaaaatt ggattgactt atgaaaaact catgaaaaaa ggttttaaga aggaagaatt
     5041 atcaaaagta catgcaccaa taggcataaa attgaatgct caaacaccag ctgaaatagc
     5101 tgtatgtata gctgctgaac ttataaattg tagagctaat aaggaaaaat aaactaatga
     5161 acacagggag taaagatatt gatactttaa aggttaattt tagagggatt gaaataaaag
     5221 cagttttagt atataaaaat agaaaaaata tttctataaa aatagatcca tttggcaaaa
     5281 taactgtaat gagtcctcct aaaatatcca aaaaaataat aaaagatata attattgaaa
     5341 aaggagactg gatacttaaa aagtcagaaa aatgtaaagg aagagaggaa gtatataaac
     5401 aaagaatgtt tataactggc gaaaaatttc tttatttagg tgaagagtac catctagtta
     5461 taaaagaact gttgagagat tctgtcaaaa aaagtaactg tagaataact attaacgaat
     5521 atcaaatagt tgttcagaca aatgatacaa gtacagattt tattaaaaaa tctttaaaat
     5581 cttggtataa gatggaaagt gaaagaatag tattagagag aatagatttt cttaaattaa
     5641 attgtgaaat aatgaagcaa ttagttccag cgagtgttaa ggtaaaagaa caaaataaga
     5701 gatggggaag ttgtacagct caaaaaaata tttatataaa ctcaaaaata tctatggcaa
     5761 gattagatgt aattgattat gtaataatac atgagtttag ccatttagca catatgaatc
     5821 actctaaaaa attctatgat ttagtaaaat ccattatgcc cgattataaa gataaagaaa
     5881 attggctaaa aataaatgga tataaaataa taatctgaaa acgattattc caaaatatag
     5941 gcgtaaagta taaaagaagt attattgtga aaaaaataaa taatctgaaa attttaaaaa
     6001 aagtcattga cattgtaaat gatagagaat ataatctata ttaacaacaa aacagaattg
     6061 aaatataaaa ttaaaaaatt aaaagcaatg acagagacaa caatactaat agtgttatcc
     6121 acagagaacc aatgttgctg agaattggtg ttaacagtag tataatgatc actctggagc
     6181 ttttgtactg aatattttta gtaagtacaa acgtatgtca acgttacatg actaaggtta
     6241 aggtaattaa ttacccgacc tgaaaaaaga ggggtagatg tattttttat atctacaaat
     6301 aaggtggtac cgcggaatat aactttcgtc cttataagta atatttatat tgcttataaa
     6361 gatgaaagtt tttttatttt aagtgatata aataaattta atccatataa aattaataaa
     6421 aaaataataa aaataaggag ggatttttat ggaaaatact gtatatgcta aattaaacca
     6481 aggaatagat tcttttttaa gagtggctat gactctaaga agaagagagg ttgatataca
     6541 atcaataagt atgactgtag ataatatgaa taattcaggt ataaaattaa tagtaaatga
     6601 agaaaagaca tccttagatt ctgtcttaaa ccatatgaaa aaactacatg atataagaga
     6661 gataactgtt gaaagaatga gacattaatt aaatatattt tataaaacta aagataaata
     6721 tgctta
//
LOCUS       DAMJVI010000152         6615 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.157, whole genome shotgun sequence.
ACCESSION   DAMJVI010000152 DAMJVI010000000
VERSION     DAMJVI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.157"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            95..997
                     /locus_tag="PC545_003418"
     CDS             95..997
                     /locus_tag="PC545_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HDF2458136.1"
                     /translation="MLPQDYYGLIVILGVILGVMGAFYNWFTLYVQSLYKKIPKIGTF
                     GKLLIPFIMAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGS
                     GAPGGIFFPLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTG
                     IILIFEMTGSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGE
                     KILDQFSVMFGSPVANKAIQDVQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMT
                     DERDSGAVYDKMENLCKEHTSHIF"
     gene            1609..2622
                     /locus_tag="PC545_003419"
     CDS             1609..2622
                     /locus_tag="PC545_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2458137.1"
                     /translation="MVSSFLNDIFRTILVSCIFIVILLVFRITLFKIFSKKFNYYIWL
                     IVIIKLSLPFMNYTFIFNESKYNGNINKISLGNFNSISIVYSTVLVSVWIAITIFYLV
                     YTLLKYIKLKNMINDLSYDVEDDKIKNLYENLLKELKINKKIRLKYSYEVESPVFFNS
                     CVILPPSDYTLKELNWIFRHELMHFKSKDLYIKYLVIFLKCVYWFNPFVYIMEKFIDL
                     DCEFYCDERVLKNCTIEEKQDYALTIISAMRKGSNSSNKFVAGLHKESDIKKRVFSMF
                     NEKYRNGILIAVILCLLSSITYFKIDLITTKSIFYPLSKQTPIENRTNSKPTYQINVY
                     IDN"
     gene            2978..3547
                     /locus_tag="PC545_003420"
     CDS             2978..3547
                     /locus_tag="PC545_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HDF2458138.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTGILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESNGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPDVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKVLYFNRYN"
     gene            complement(3883..5220)
                     /locus_tag="PC545_003421"
     CDS             complement(3883..5220)
                     /locus_tag="PC545_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDF2458139.1"
                     /translation="MGTYSLISTSINFILILINIFIAFFLYTLTSYFIDFRNHWIFKI
                     ILILGYSIISQMIIWLSDPVNILFTLFGYFILLIICSKSKLIPIISVVMIFYPIIIGL
                     NFLFINNPIYEELIHSKEYILILSIVQIISALIKLLFWFLIHIFLKNRLVNIKQYLSN
                     KIWIFIDMVCISSFLSILIAIILPPTNLIPMGSNKNIILGNLPYGTYLIILSAILANI
                     GVILLLQPLIENVKMKIEKQANHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLE
                     IEDIAGAKKYFNQLKIGIGTLSGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDN
                     HLGIEPIDLCTIFSNSLDNAIEASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPI
                     NRKQELFISDKKDSNSHGYGIENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(5664..6374)
                     /locus_tag="PC545_003422"
     CDS             complement(5664..6374)
                     /locus_tag="PC545_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458140.1"
                     /translation="MTSICICDDEKQQRNMLNKIITREMDLWGYPYQIKEYSNGESLV
                     YNLEHENYYIDIIFLDIELGKSNGVDIAKTIRKHSKDTIIIFVTGFSDYVFHGYDVGA
                     LNYILKPYKTEKICKVLKEALKKLEQWKENFITVQIGTNLCKINTKDILYFKSELRKL
                     TVVTFDKTLEYYGKLNDMETVLPSNFIRTHQRYIVNLNHVDSITQTYAKVQNEKIPIS
                     RKKYQEVVGKFTRYMLES"
BASE COUNT         2445 a          699 c         1018 g         2453 t
ORIGIN      
        1 gcctgtggag aattagtaag acatatgtta gtatgcaaaa ttctatgaag caggaaccct
       61 gtgactttag tcatgggagg ttcagattgg aaatgtgttg ccacaagatt attatggatt
      121 aatagtgatt ttaggtgtta ttcttggagt tatgggggca ttttacaact ggtttactct
      181 atatgtgcag tctttataca agaagattcc taaaattggt acgtttggaa aactgttgat
      241 tccatttata atggctggta ttcttgggct tgttatgcct gatgttctgg ggagtggaca
      301 caatttagtt gaagaactta caagtcatga atatattttt ggaatggttg ttttgatttt
      361 tgttatacgt tttatatttt cggctgttag ttttgggtca ggtgcaccag ggggaatatt
      421 ttttccattg cttgttttag gagcatttat aggtggaata tttggtatgg taggtgttaa
      481 agtttttgga atgaatcccg attatgtaaa taactttgtg cttttggcta tggcaggata
      541 ttttacagct attgtgcgtg cccctttgac tggtatcata ttaatttttg aaatgacagg
      601 ttctgttagt caaatgcttt cactatcagt aatttcaatt gtggcatata ttgtggctac
      661 acttatgaag tctaaaccta tttatgaaag tcttcttgag aggttacttg aaaaaaatgg
      721 agaacctgta cataaagaga ggggcgaaaa gatattagac cagttctctg ttatgtttgg
      781 ctctcctgtt gcgaacaagg caattcagga tgtacagtgg cctgaaaatt gtcttttggt
      841 agcaattcaa agaggtggag aagaaattat tcctagaggt aagacaattc ttagaccaag
      901 tgatgtaatt gtgactatga cagatgagag agatagtggt gctgtgtatg ataagatgga
      961 aaatttgtgt aaagaacata cgtcacatat tttttaggga taatgatgaa tgtttaataa
     1021 tgatagaata tttaatgaag atttgattaa aaaattggta atttgaaaga gagttaagga
     1081 attaatataa gaaaatgaaa atgtgatatc ttagatgtaa aagagtttga agaaaataat
     1141 tgtatatatt tgttaatgag attatatgga ctaatggtat atttatactg tgctaaaagt
     1201 tgcactattg tatgaggaat gggaataaag ggtttgaaaa aataggtaga agatttttca
     1261 gatattctta ctgggatatt ttaattaata tattttaatt gcgatatttt gggaagaata
     1321 gaatttattt ataaatgaaa ataattaaaa ggggttaagg ttgtattagc cccttttgct
     1381 tatagagttt ggaaatggta tcttgatagc agttgtttca tgcttattat attttattac
     1441 atattttaaa attgacctta ttactcctaa atcattgtta tatcaacttt taaaacactt
     1501 tcaagacaat cgcctattga aaacaaagtt aattaaaaac ctacatatca gtttagtatt
     1561 cgtgtagatg attatataaa tataaaagta taagaaaggt gagtatctgt ggttagttca
     1621 tttttaaatg atatctttag aacaatttta gtaagttgta tttttatagt tattttatta
     1681 gtctttagaa taactttatt taagattttc agtaaaaaat ttaattacta tatatggctt
     1741 attgtgatta ttaagctatc attaccattt atgaattata cttttatatt taatgagtca
     1801 aaatataatg gtaatattaa taagataagt ttaggcaatt ttaatagtat ttctattgta
     1861 tatagtactg ttttagtctc tgtttggatt gctattacga tattttattt agtttatact
     1921 ttattgaaat atattaagct taaaaatatg ataaatgatt tgtcttatga tgttgaggat
     1981 gataagataa aaaatcttta tgaaaattta ttaaaagagc ttaagataaa taaaaaaata
     2041 cggcttaaat attcatatga agttgaaagt ccagtatttt ttaattcgtg tgtcatttta
     2101 ccaccaagtg attatacatt aaaagagtta aactggatat ttaggcatga gttgatgcat
     2161 tttaagagta aagaccttta tattaagtat ttggtgatat ttttaaaatg tgtttattgg
     2221 tttaatcctt ttgtgtatat aatggagaag tttatagatt tggattgtga attttattgt
     2281 gatgaaagag ttttgaagaa ttgtacgata gaagagaagc aggattatgc attgacaata
     2341 attagtgcaa tgagaaaagg ttcaaatagt tcaaataaat ttgtggctgg attacataag
     2401 gagtcagata ttaaaaaaag agtgtttagt atgtttaatg aaaagtacag aaatggtatt
     2461 ttgatagcag ttattttatg tttattatct tctattacat attttaaaat tgatttaatt
     2521 actactaaat caatatttta tccactttca aaacaaacac ctattgaaaa cagaactaat
     2581 tcaaaaccta catatcagat taatgtttat atagataatt aaaaaataca aagtttaaca
     2641 agagattaat ataaataatt ttaataagta tctttcacat atgttaaatt tactttaatt
     2701 atattaaagc caatgaatta ttctttagaa gctccaatgc cagtaaatac aggtaaagaa
     2761 aaatatttta ctacaattgc agattaaaaa gtgttctaat atttaaagaa atacacaagt
     2821 gtaagaaggg taaaaatctg ttataataca ttaaaaacta aatttagaaa aatttacata
     2881 attagaaaaa tgatttgata aaaaataaag ttaaatatta actaagtaaa tttgaaaaag
     2941 aaaaaatata aagtacaaat ttaaaaggag gtaactaatg aaaaagaagt tggaagatat
     3001 gactttagag gagttatgga acttgtttcc tatatttttg actactcaca aagaatgttg
     3061 gaaaaattgg tttaaagaag aacaatatag actaactgga atattgccaa tgcaaaaaat
     3121 tgttagaata agtcatattg gaagtacttc tgtgagtacg atttatgcca agcccattgt
     3181 tgacatactg attgagataa ttcctgatta taatatgaaa gatattaaag atttgattga
     3241 gtcgaatgga tatatttgta tgtctgagag cagtagtagg ctttcactta ataaagggta
     3301 tacagtagat gggtttgaag aacgagtttt tcatttgcat ataaggtatg taggtgataa
     3361 tgatgagttg ttttttagag attacttaat tgaccaccca gatgttgcaa aggagtatga
     3421 aaaacttaag ttggaccttt ggaagaagta tgagcataat cgtgatgctt atacagatag
     3481 taaactagat tttgttaggg agtttactga aaaggcaaag gtgttgtatt ttaatagata
     3541 taattagaaa taggtacaat tagatattta tcttatatat cgtattatat tttttaagaa
     3601 agtaattaat agaaaaaggg gttgtttcaa aatggagaga taaatccctt tttcattgtt
     3661 gaaaggataa aaatatattt gatttcatgt aaaaaagagt gcctcatcaa ctaaaaagtt
     3721 tattttgaga cacacacatt tttactaagg taaataaaat atcacatttg attaaatttt
     3781 aagttaatga atatatgtgt aaatatttaa ttataagtta ttataatttt acatagtatc
     3841 aaacaatcta atttgtatta tataagataa tctaatttta gactaaaata atctaatata
     3901 aagaaataaa gaaaattcat tctcagtata agagatatcc aattttccat tgtacttatt
     3961 aacaatttct ctaatattct caataccata tccgtgagaa ttactatctt ttttatctga
     4021 aataaatagt tcttgttttc tattgatagg atttaccttg ttgtttgtca ataaatagct
     4081 aaaatactcc ttctcacaac gtgcctttaa taccactttt ctatttgaca aatcaggaat
     4141 cttcaaagat gcttctatag cgttatctaa agaatttgaa aaaatagtac ataaatctat
     4201 aggttctata cccaagtgat tgtctatatc tatattaaag tgcacatcaa caccattttc
     4261 ttccaaactt gtatgacgaa gatttaaaat tgagttaaga gcttggtttt tgcaaaactg
     4321 tttgccacta agagtaccta tacctatttt taactgatta aaatactttt tagctccagc
     4381 aatatcttca atttctaaat acgattcaat catttgaaag tgattattca tatcatgtcg
     4441 cattttgcgt atttgaattt gttgattttc tagtaagtta tagtattttt cttttagatg
     4501 atttgcttgt ttttctatct tcatctttac attttcaatc aatggctgta aaagaagtat
     4561 cacaccgata tttgccagaa ttgctgacaa gatgattaga taagtgccat aaggtaagtt
     4621 gcctaagata atattcttat tactacccat agggattaaa ttagtaggtg gcagaataat
     4681 agcaattaat attgataaaa atgaagatat acataccata tcaataaaaa tccatatttt
     4741 atttgataag tattgtttta tattgaccaa tctattcttt aggaaaatat gtattaaaaa
     4801 ccaaaataat aatttaatta gagcagaaat tatctggact attgaaagta ttaaaatata
     4861 ctcttttgag tgtataagtt cctcatatat agggttgttt ataaaaagaa aattaagccc
     4921 aattataata ggataaaata tcatgacaac tgatattata ggaattaatt tgcttttgct
     4981 acatattata agtagtataa aatatccgaa tagtgtaaac aaaatattta cggggtcgga
     5041 aagccatatt atcatttgac taataataga gtagcccaaa atcaatatga ttttgaaaat
     5101 ccaatgattc ctaaagtcta taaaatagga agtcaacgta taaagaaaaa aggctatgaa
     5161 aatattgatt aatattagaa taaaatttat gcttgtagag attaaagaat aagtgcccat
     5221 ttaatatacc tttctattaa ttaagattct ggaaactaag ttttaaaaaa ttaaatctta
     5281 aaaattaaaa ttctggaaat taaatttcaa agaattgatt ttgaaaaata aaattctaga
     5341 gattgaactt caaaaaattg agttttaaaa gattgaaatt ttaaaaatta agtttcaaaa
     5401 aattaaattt gaaaaaaaac tctaaaaact aagcttcaaa aaattaaatt ttgaaagatt
     5461 gaaattctag aaactaagct ttaaaaaatt aagtttgaaa aaacaaaact ctaaaaacta
     5521 agcttcaaaa aattaaattt tgaaagattg aaattctaga aattaatctt caaaaaatta
     5581 aatttgaaaa aacaaaactc taaaaactaa gctttaaaaa attaagtttc aaaaataaaa
     5641 ttctagagat tgaacttcaa aaattaagat tctaacatat accttgtaaa ttttcctaca
     5701 acctcttgat attttttacg gcttatagga attttttcat tctgaacttt ggcataagtt
     5761 tgagttatac tatctacatg gtttagattg acaatatacc tttgatgcgt acgtataaaa
     5821 ttagagggaa gaacagtttc catatcattt aattttccgt agtattctaa tgttttatca
     5881 aaggtaacta cagttaattt tcttagctca cttttaaagt agaggatatc ttttgtatta
     5941 attttacaaa gattagttcc aatctgaact gtaataaagt tttctttcca ttgttctaat
     6001 ttttttagtg cttcttttaa tactttacat attttttcag ttttataagg ttttaagatg
     6061 tagttcaaag ctcctacatc atatccatga aatacatagt cagaaaaacc tgtgacaaag
     6121 atgattatag tatcctttga atgttttcgt atagtttttg ctatgtcaac gccattactt
     6181 tttccaagtt caatatctaa aaaaataata tctatgtaat aattttcgtg ttctagattg
     6241 tatactagac tttctccatt tgaatattct tttatttggt aggggtagcc ccacaaatcc
     6301 atttctcttg taatgatttt attcagcatg tttctttgtt gtttttcatc atcacaaata
     6361 caaatcgaag tcaatttttt atctcctttc aaattttata tttatatagt taagtatata
     6421 atatattttg ctacctagat atataaaatg aaataacaat tatatccaga tagcaaaata
     6481 aaatttttta tattagtata cttacactta aaactattct attaaactta ttctgaagtg
     6541 ctaatgattt tcttattaaa aaatctaaca atctttctta ttataaaata caaatgcttt
     6601 tattattata aaaag
//
LOCUS       DAMJVI010000153         6462 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.158, whole genome shotgun sequence.
ACCESSION   DAMJVI010000153 DAMJVI010000000
VERSION     DAMJVI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.158"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            178..1581
                     /locus_tag="PC545_003423"
     CDS             178..1581
                     /locus_tag="PC545_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2458141.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKLNMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1672..1912
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1955..2653
                     /locus_tag="PC545_003424"
     CDS             1955..2653
                     /locus_tag="PC545_003424"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDF2458142.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2676..3677
                     /gene="trpS"
                     /locus_tag="PC545_003425"
     CDS             2676..3677
                     /gene="trpS"
                     /locus_tag="PC545_003425"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDF2458143.1"
                     /translation="MSEKKVILSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANTIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4329..5896)
                     /locus_tag="PC545_003426"
                     /pseudo
     CDS             complement(4329..5896)
                     /locus_tag="PC545_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iota toxin protein Ib"
     gene            complement(5871..6014)
                     /locus_tag="PC545_003427"
     CDS             complement(5871..6014)
                     /locus_tag="PC545_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458144.1"
                     /translation="MYVYYFESPEKFAFNKVIRTKNQNEISLEKFNEFKETIQDKLLP
                     YNR"
     gene            complement(6232..6414)
                     /locus_tag="PC545_003428"
     CDS             complement(6232..6414)
                     /locus_tag="PC545_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458145.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSGKV
                     CNTTYKAPIERSEGFF"
BASE COUNT         2285 a          773 c          997 g         2407 t
ORIGIN      
        1 cttttaaatt tatcattttt aataagattt gatttcaaag tatttaaggt ttattattaa
       61 aactgttttt gaatatgcaa atattttgaa aaatatcagt ttaataataa tgaatctatt
      121 ttattaattt tgaaattaaa tttaaaataa ttaatagaga ggggaaatta aattaggatg
      181 gaaaagaaaa aaataggcct atggagtttg gtatttatga atgtatcagc tttatatggt
      241 ataagatgga tagctaaatc tacagcatca agctttggtt taggattagg tgcaatacct
      301 atgtggttat tattctcatt tatatacttt gtaccaggag ctttaatttg tgctgaatta
      361 gcttctacat atcaaaaagg tgatggaggt ctttacgatt gggttaagga agcctttgga
      421 gaaaagtatg gatttttagt ttcttggtta aactggacag ctaaaatatt ttggtattct
      481 tcatttttaa ctttcttagc agtaaatgtt tcttatacaa taggaaatcc agcccttgct
      541 gataataaga tgtttgtact tatattatct ttaataatat tctgggcatt atctcttata
      601 gcaactaaag gaataagttt tacaaaaata tttactaata caggtgcatt aggttcaaca
      661 ataccaagtg ctatacttat aatattttca tttgtagcag tatttgtatt gaaaaaacat
      721 gatatagctt ctacatacac aatagcttca atgactccaa aacttaatat ggatgcattt
      781 gttgctatat cagctataat gtttggttta gcaggagcag agactgctgc taacttcata
      841 acagaaatag ataagcctga aaagaacttc cctaaggcaa tattaatatc agctggaata
      901 gtagcttcat tatatgtatt aggttcaatt gcaataacaa tgataatacc accagaccaa
      961 attactgctt ctaaaggtgt attagatgca ctttctgctg tttgtgcgtc tttaggaatt
     1021 ggttcttggt tggttcaatt aatagctttt ggtatagctt tttcagtatt aggagcaata
     1081 gttctttaca tagcatcacc aataaaaatg ttatttggta gtgttaaaaa aggaatattt
     1141 ccagatagtt tagtagaggt aaatgaacac aagatacctt ctaaagcagt tatacttcaa
     1201 gcaataatag ttactataat actattggtg actacattaa tgccttctgt tgatgctata
     1261 tacaatgttc ttgtaactat gacagcatta actgcattat tcccatatgt attgttatat
     1321 gcttcttata ttaaactaag aaaagagaga ccagatgaaa ttagacctta tactatggca
     1381 aaatcaacaa gtacttgcct aggtttagca aaaatggttt tagttgtaac tgtagtgggt
     1441 atattattat ctgcagcacc agttatgcct acatttgctg aaaatgttat atatgagata
     1501 gagatgattg gtggaggtct tctagttata ttatctggtc tatggttatg gaatagatat
     1561 gaaaagaaaa cagctaaata aaatgtattt taaaaatttc ttttggatat tataatatat
     1621 agttgacaat ttatagtgta aggaatatta taaaataaag aaattaataa attaaatcct
     1681 gagataagaa atagtaaata aatatatttt tgcacagaga attaaggatg ctgagaactt
     1741 aataaaaagt atttattgaa tgggtctttg aggagtaaaa tgaaatgtaa tttaagatta
     1801 cagagtattt gatatcgtgt atgcacgtta tagcatatat taagatatat atattcaatt
     1861 ttgagtgtat ataaatttag gtggtaccac ggaaaatata tccgtcctat tgatttagga
     1921 cggatttttt aatatagaaa atttaggagg aaaaatgaac gtaataggaa ttataggtgc
     1981 aatggatgaa gaagttagta ttttagttga tttaatggat attagagaga ctataaaaaa
     2041 agctagctta gaattttata agggaatttt ggaaggtaag aatgtagtgt tagtaaagtg
     2101 tggcataggt aaggttaact ctgctctttg tgcccagata ctaataagcg aatttaaggt
     2161 tgatgctatt gttaatacag gtgttgcagg agctttaaac gaaaaacttg atgttaatga
     2221 tatagtaata tcaactgatg ctatacagta tgatgtagac actacagcat ttggagaccc
     2281 taagggagtt ataccaagaa tgaagacttc tgtatttaaa gcagatgaaa gacttataga
     2341 tgctgcttac aaatcttctg tggaagaagt aaagactcat aaagtattaa aaggaagagt
     2401 agttacagga gataaattta taaattctaa agaattaaaa gaagaattag taaatgattt
     2461 tggtggatat tgtggagaaa tggaaggtgg agctatagct catgtatgct atttaaataa
     2521 tactccattt gtaataataa gagcaatgtc tgataaggca gatggaagtg ctgatgttac
     2581 ttatgatgta tttgtacatg atgctgccaa taactctaaa gacatagttt taaatatgtt
     2641 aaaatcaata taaatatata aggaggtaca aaaaaatgag tgaaaaaaaa gttatattaa
     2701 gtggtgcaca gccatcaggc aaaatgactt taggaaatta tttaggagct ataaaaaact
     2761 ggactgaact tcaagacaat tatgattgtt attatagtat agttgatttg catgcaataa
     2821 cagtacctca agaccctaaa gttttaagag ccaatactat agaattactt gctcaatata
     2881 tagcttgtgg attagaccca gaaaaaaata ctatatttat acaatcacat gtcaaagagc
     2941 atgttgaact tatgtgggta ttgaatacta tgacttatat gggtgaatta agtagaatga
     3001 ctcaattcaa agataaatca caaaagagtg aagcaaatct aaatgcaggt ttattcacat
     3061 atccagttct tatggctgct gacatattgt tgtatcaaac tgacctagtt ccagttggag
     3121 atgaccaaaa acaacattta gaattagctc gtgatttagc aaataggttt aataatagat
     3181 tttcacctac ttttgtagtt ccagaaggat actacccaaa aggtggagca agagtgatga
     3241 gtttgcaaga gccaactaaa aaaatgagta aatccgattc aaatgaaaat gcatttatac
     3301 tattagcaga tgattcagat tctataaaga gaaagataaa aagaagtgtt actgattctt
     3361 taggtgttgt aaaatacaat gatgaacaac caggtattaa aaacctaatt gatatatatt
     3421 ctaatttaag taaaaagagt gtagaagaga ttgtaaacat gtatgaagga aagggttatg
     3481 gcatatttaa agaagatgtg gctgaagtag tatcagaagc tttaagacct ataagagaaa
     3541 aatatgttga ccttcttaat aataaagatt atttagaaaa aatatatagt atgggagcag
     3601 aaaaagccga aaaacaagct agaaaaactc ttagaaaagt ttataaaaaa gttggattta
     3661 tagagagaag atactaatta ttagctatct ttacttgata aataaatttt aaagttatta
     3721 aaaaggtata tgatatgaaa gagagagtct caaagtgaac ttttgttcat gaggcactct
     3781 tttttgtatg aaataaaata tattttaatc atttcaacaa tgaaaaagag gattatctct
     3841 attttgagac agcatctttt gataataaaa aaagatgagc ttttgctcat ctcaaagggg
     3901 ttagattatt cttactttgg ggaaagtaga ataaaggtaa aagttaactg atgtcaactt
     3961 tgtctttttt gcatatcact actgagcata aaaaaatcag aaaaccatag ccttctgaaa
     4021 caacttctaa catacattgt attttatacc caatatgtaa tttatgagtt aattttaact
     4081 cttataatga aaattgtcaa taatatatca aaaaaatatt ttttaaattt atataattaa
     4141 atgaatttat gtttatatat aggtgatatg atacagataa ttatcttttt aatacaatat
     4201 agttctaaaa aagaatatat tagaagaaga taatatttaa ataaaaaata ccttataaag
     4261 ttaatttttt aatactttat aaggtaaata catattgtat tgtggggata aatttaaatc
     4321 atttgatact aatcaacact aagaaccaat aactctctat catcttgagt aattgtatat
     4381 attcttaatt tcttgtatgt cataatattt tctccacctg taaaataact cctaagatta
     4441 acacaatcag ttttaggttg atttgtatct cctaaataat tacgcaattc tttatcttta
     4501 gtttcaatat ctttaaatcc aaaatattgt aaagtagctt caactctata tttttggaca
     4561 taatcgagag cttctttatt agtttatgtt ggtgtaaaat acataccatt tatataagcc
     4621 tttattgtac ttggattaaa tttaaaatct gcagaatata ttttatgtgt aactattatt
     4681 tcttgaacag ttggaatttt aacttctggt tcatcaagta tttcaggagt actatttagc
     4741 tctgtaatag ataagttatc taagtatatt gtatcactat caattaatgc tatatttata
     4801 ttagaagaat ctttttcagt agtttcaaat tcataactaa attttgtata tccttgttct
     4861 agtaccaaat aatccgtttt ttcttctttt acttttatat ttacaattat tgaattatat
     4921 gttaaaggat tttttgaatt ttactaaaaa cataacgttt ataaggtttt agttcagaca
     4981 taggtatttt aatcttcgtc tcaccattta atctatttgc tgagcctttt aaaccatctt
     5041 gatttgtagt attaacatta ctccatatac tagggtaatt attgtaatca tcaaaattag
     5101 taaagtaagt tggtgttttt ataagtatat tcattcctct ttcaagttta acattacata
     5161 tttgtttatc agacaaagtt tttaaactgt ctttaatctt attgcctata ttatcatcaa
     5221 atatgagttc aacacaactt tcatctattg gtatattatt aaaatataac aaaccatctt
     5281 ttttagtagt gccaaaagtt ttttcaattg cttctccaat tgtaagttca ggtgttttat
     5341 cttctgggac ctgtaaactt ttagcagtaa ttcttctttt gtaagattca ttttctgtat
     5401 ctaatataat agatacagaa atactgtcaa tttgacctat atcgtcttac caactatttc
     5461 attctgttac tatttgtcca gaaccatttt ttgtaccaaa atttccactt acttgtgttg
     5521 tttctaattt aatttgcttt ccatcatcta atttttttaa ttaattataa tttataggaa
     5581 tcagtctaga actaaattga tacattgtgt taagagccat aggtgaaaat ccttttttga
     5641 gataagtatt tccaggagat agattattgc caatttgatt ttcttgtgct ttgatagttg
     5701 ataatgcatc tccatctaac actaaatttg ttgttggtgt cactttgtac ataggtgcag
     5761 tacctgtgtt gtagtatcta atatttgcat ttatatatgc agattctcct ttgtttatac
     5821 ttaatccagt atttcatgat tatccattgc tattttgaac agcagttgaa ttatctgttg
     5881 tatggtaata atttgtcttg tatagtttct ttaaactcat taaatttttc taatgaaatt
     5941 tcgttttgat tttttgttct tattacttta ttaaatgcaa atttttctgg agattcaaaa
     6001 taatatacat acataggttt atctatttta ttttttgata tagcatttcg aagttctcta
     6061 tattcttttt ctcaagaatt tgcttctatt tgataatcat aaaaataatt tcttgtctga
     6121 gaatatttgc ttatctctat agaatctttt ttatatgatc ctaatacttc tttttcagat
     6181 ttttcaagtt tttgatcttt tctttctcat tctttagcct tttctttatc tttaaaaaaa
     6241 tccttctgat ctttctatag gagccttgta agtagtatta caaacttttc cactatagct
     6301 ttgtaaatct tgtgcataaa tgttattagg taacaaacta cctaaagtta gatataatat
     6361 caacaatata gatatacaat tactaatact tttatgtttc ctaaattttt tcatttattc
     6421 tccctacaaa tattagttta gttaactttt gaattttaat tt
//
LOCUS       DAMJVI010000154         6412 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.159, whole genome shotgun sequence.
ACCESSION   DAMJVI010000154 DAMJVI010000000
VERSION     DAMJVI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.159"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            211..1536
                     /gene="cdeA"
                     /locus_tag="PC545_003429"
     CDS             211..1536
                     /gene="cdeA"
                     /locus_tag="PC545_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HDF2458146.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQDEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATDKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGIIYFLSNKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGITTFLFNVVALKLAGENGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFISSLLVFIIALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNMTGLWLTVPFAEVITIFISILFIKKYKCRYKY"
     gene            complement(1631..2425)
                     /locus_tag="PC545_003430"
     CDS             complement(1631..2425)
                     /locus_tag="PC545_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458147.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIVGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYSIELSVLLKRDMIWTSISALIDPSTSSMPLTYLNYF
                     VQICINEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(2426..3301)
                     /locus_tag="PC545_003431"
     CDS             complement(2426..3301)
                     /locus_tag="PC545_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458148.1"
                     /translation="MSSSIKIENLSHSFKKQNIFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQIFEFSSS
                     FYKSYDKGLEKELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKNIKPKKAFGNAVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(3305..3673)
                     /locus_tag="PC545_003432"
     CDS             complement(3305..3673)
                     /locus_tag="PC545_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDF2458149.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFVKNLLQEA
                     NKLEINREELVDIIINFKED"
     gene            complement(3856..4515)
                     /gene="deoC"
                     /locus_tag="PC545_003433"
     CDS             complement(3856..4515)
                     /gene="deoC"
                     /locus_tag="PC545_003433"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDF2458150.1"
                     /translation="MKHILKTVDHTILKATTTWEDIKILCDEAVDMSVASVCIPPSYV
                     KRASEYLKGKIKICTVIGFPLGYQTTATKVFEAKDAIENGADEVDMVVNISDIKNKDY
                     DNIGKEIKEIKKAIGDKVLKVIIETCYLDEDEKIKMCEIVTMSGSDFIKTSTGMGTGG
                     ATLEDIKLMKEHVGKNVKIKAAGGVKSISDAEKFIEAGAERLGTSSICKILKNEDTTD
                     Y"
     gene            complement(4551..5738)
                     /gene="murJ"
                     /locus_tag="PC545_003434"
     CDS             complement(4551..5738)
                     /gene="murJ"
                     /locus_tag="PC545_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDF2458151.1"
                     /translation="MKQEPCDFSHGRFRTGIIFIGITSVMSAFLQIKENFIVVGFGSI
                     PYNIVIIISIMLSTIFGPYILPIGAVVAMVVQLLFYMFFVKKTNYKYLYYLNFKDDSL
                     IKLLALLSPVFIGVAVNQVNSLVDTTLASTLVKGSIPALTYADRLNGFVTGTFTASIV
                     SVMYPMLSKLSAENNQKKFTSSVKSSINMIIISMIPISVASIFFATPVVRIIFERGAF
                     DARATQMTATALIFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIISVGVNIVLDLV
                     LIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIKATLKSVVASLIMGVL
                     SYFTYKFIFGILGVGTFNEFVSLAISVIVGGGIYTLLMTIFKVEEVDMILNIAKRKLH
                     LKK"
     gene            complement(5815..>6412)
                     /locus_tag="PC545_003435"
     CDS             complement(5815..>6412)
                     /locus_tag="PC545_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2458152.1"
                     /translation="FENTNNQIGLDLGIKEFCISSCGEFIENPKYFKKSLNKLAKLQR
                     ELSRKTIGSLNRNKARLKVARLQEHIANQRNDFLQKLTTKLIKENDIICIEDLQVKNM
                     IKNHKLSRLISDVSWSEFIRQLEYKANWHGRQIVKVGKFFASSQICNKCGYKNEEVKN
                     LNIREWICPSCNETHDRDINASRNILKEGLRLITIQNK"
BASE COUNT         2121 a         1001 c          700 g         2590 t
ORIGIN      
        1 ttaagattat atacatatat ttctaaagat tttcttaaga aacagttaga ttttactaat
       61 ataagtttat taattagcat tatactaatt tatcttaata tgattttttg ttgattttaa
      121 gctgtttatc gtataaataa aatttttctt gactatatag ttactatata gcttattata
      181 tatagagagt aataaaagga ggtaaaattt atggaaaatt tatttacaag aaaattcact
      241 acttttgaat ttctaaaatt tgtttctcct gcaattatat ccatgatatt tatatctttg
      301 tatacaataa tagatggcat ctttgtatcg acattagttg gttctgatgc tcttgctagt
      361 ataaatattg tactacctat aattaacctt gtttgtggat ttggaataat gatggcaact
      421 ggtggaggtg ctatcgtttc tatacgtatg ggtgaaaata gacaggatga agccaactct
      481 acattttctt ttatagtttt gttttcattg attgttggga ttttattcac agtaatctca
      541 tatttcttta tcaaagaaat atctattttg cttggtgcaa cagataagtt attaccatat
      601 tgtataactt atggtaaagt tatgatttta tgtacaccat tttatatttt aaaatttata
      661 tttgagtact ttgcaagaac tgatggaaat tctaaattta gtttatttct atcagtcatt
      721 ggtggtgtaa caaatataat attggattat gtatttatta aatattttgg aatgggtctt
      781 ttaggagctg cagttgcaac tgctataggt attattttaa cttgtgtttt aggtattatt
      841 tacttcttat ctaataaatc tacactaaaa ttaagaaaac caaaaaccga ttttagactt
      901 ataagagata ctatgataaa cggttcttct gaaatggtta cagaattatc tacaggaatt
      961 acaacattct tatttaatgt agtagcttta aaattagcag gagaaaatgg acttgctgct
     1021 cttactatag tattgtatgc tcatttttta atgacatcag tctatctagg attcgctgct
     1081 ggagtgtctc cattaataag ctataatttt ggtgctgaaa acagtgataa attaaaagaa
     1141 acatttaaac attctctaaa atttatattt atttcttctc ttttagtgtt tattattgct
     1201 ttagtatttg caccatttat tgttagggtc tttgtaaatc cagataacac agtatttaaa
     1261 ctagccttac aaggattaaa aatatttgca tttgcttttt tgtttgttgg tataaatata
     1321 tttgcatcag gattttttac agcatttcac aatggaaaaa tttcagctat tatatctttt
     1381 agtcgtgcct ttgtttttat aatcatagga atcataattc ttcctcctat gttaaacatg
     1441 actggattat ggcttacagt tccatttgct gaggttataa ccatatttat atctattcta
     1501 tttataaaaa aatataaatg tagatataag tattaaaaat taatacttaa aatcaacatt
     1561 tattaaaact aataaacctg ctttaaaata caaatttaca aatttgattt ttaagcaggt
     1621 tttattttag ttataataag tcatttgcat actctttaat tggtgctttt ctaagtaata
     1681 aaattataaa tattatgcaa ataactataa ttcctacctt tgttccaaat aaaataaaag
     1741 gattctgaaa tgaataaata attttttcta aatacaatag cattttatgc atatttttag
     1801 ttgcaaaatt aactatataa attaccatta atatcggtaa tcctatcaat atagtaatac
     1861 tagtaacttt tctaactcta tatgtaaaag ctccaagtaa cactcctata aaacctatac
     1921 atatttcatt tatacatatt tgtacaaaat aatttaaata agttagtggc atacttgatg
     1981 ttgaaggatc tatcagtgct gatatagatg tccaaatcat atctctcttt agtaaaactg
     2041 atagttctat actgtaacct gttataattt ctataaataa cttcgataaa aaaatcaaaa
     2101 ctatacctaa aatagaaatc acaaagctta aaataattaa atctatgaca atagctttta
     2161 tacaactttt tctatctgct cttatactta aagctcctga aaagtcttta tttgctatat
     2221 ttactccata cacaaatagc agtatagtta tatatgttaa aataggattc acaatcgtgg
     2281 ataaattagt tttagatgct tctccaaaaa tcttgagaat aaacactggc aaaatattca
     2341 taacaattcc cacaatcata aatataatta tatacaattt agtattttta ttaaaaaatt
     2401 ttatatatgg ctttaattca ttcaattaca attcctcctt cttagtcatg ttaacaaaca
     2461 tatcttgtag ccctatgtat cctacatcaa tatttgaatt tttaagtatt ttttcatcat
     2521 tttcactaaa atcaccatag taaaaataag caactgcatt tccaaatgcc tttttaggtt
     2581 ttatattttt tatacattct aatttttcta actcttcttt atttccagta atataatgag
     2641 ctttttgttt tatgacatca atttcttcat ctataataat ttttccctca tttaatatta
     2701 ttacatgctc cagtaaatca tctatttcat caatcaaatg agttgatatt attattgtcc
     2761 ttggattttt tatataacta tctaaaatta tgttataaaa ctcttgtcta tttactgcat
     2821 ccaaaccaat tgttggctca tcaaatatgg tgatagctga attagaacaa atacctatta
     2881 tatttgaaag tatagttttc atacctcttg acagttgttt atactttttt ttagtattta
     2941 aatcaaaata tttgcataat tctttttcta agcctttatc ataactttta taaaaactag
     3001 aagaaaattc aaatatctga cctactttaa aatctggact ataaaattct ttttccctga
     3061 caatacaaac ttcttccaaa acttttatat cttcttttgg gttctttcct aatatttcta
     3121 tctcaccttc attttgaatt aactgattta ccattatatt taataatgtt gtttttccag
     3181 ctccattctt acctaataaa ccatatatct tatttttttc aaagtttaga gacatattat
     3241 taaaaatatt ttgcttctta aaactgtgag ataaattttc tatcttaata gaactactca
     3301 tatattaatc ctccttgaaa ttaattataa tatcaaccaa ttcttctcta tttatttcta
     3361 atttgttagc ttcttgaagt aggtttttta caaaattatg tttaaaattt tcttttctag
     3421 cttcttttat aatagttttt gcaccatctg atacaaacat ccctatcccc ctcttcttat
     3481 ataaaatatt tttatcaacc aatatattta taccttttaa tactgtagct ggattaattt
     3541 tataaagctt tgaaagctct gttgttgatg gaacctgttc ttcttctttt attccatttg
     3601 atagtatctc atcttcaatt gctcgtgcta tctgaataaa tataggttct tcattattta
     3661 atactaattt cataggttcc tcctttctcc attagttagt tactcaacta attaactata
     3721 taacttataa tatcttgaaa aatattttct gtcaatacta aataaaaaac agagggaaaa
     3781 ttccctctgt tttttgttct ttatatttat aattaaattt tgatttttgt aatcctatcc
     3841 cataagttaa aaactttaat aatctgttgt atcttcattt tttaatattt tacatattga
     3901 gctagttcct aacctttctg ctcctgcctc tataaatttt tctgcatcag aaattgattt
     3961 aactccccct gcagctttta tttttacatt ttttccaaca tgctctttca taagctttat
     4021 atcttctaaa gtagctccac ctgttcccat tccagtagat gtttttataa aatctgaacc
     4081 tgacattgtt actatttcac acatttttat tttttcatct tcatctaaat aacatgtctc
     4141 tataatgact tttaatactt tatctcctat agcttttttt atttctttta tttcttttcc
     4201 tatattatca taatctttat tttttatatc tgaaatgttt acaaccatat caacttcatc
     4261 tgctccattt tcaatagcat cttttgcttc aaatactttt gtagcagtag tttgatatcc
     4321 caaagggaac cctatcactg tacatatctt tattttacct tttagatatt ctgatgctct
     4381 tttcacatat gatggtggta tgcaaactga tgctacactc atatctacag cttcatcaca
     4441 taaaatttta atatcttccc atgtagttgt agcttttaaa atagtatggt ctactgtttt
     4501 taatatatgt ttcataaaat cctcctaaat ttataattaa tataattaga ttactttttt
     4561 aaatgcaatt ttctctttgc aatattcagt atcatatcaa cctcttcaac tttaaaaata
     4621 gtcattagta gtgtataaat tccacctcca actataactg aaatagctag tgagacaaac
     4681 tcattaaatg ttcctactcc caaaattcca aatataaatt tatacgtaaa gtatgataaa
     4741 acacccataa ttaaagatgc aactaccgat ttcaaagttg cttttattat tttatcttgt
     4801 ccaaaatatc caacttttct ctttaggttc aagaagagta acaatataca tgcaattgat
     4861 gatgaactag ttgcaagagc aagaccacca tgagccattg gttttattaa aactaagtcc
     4921 aatactatat ttacccctac agatataatc ccatttacca taggtgtttt tgtgtcttgt
     4981 aatgaataaa aaacctttcc caaaatatct cttaagccaa atgcagtcat accaactgca
     5041 taaaaaatta aggcagttgc tgtcatttgg gttgccctcg catcaaaagc acctctctca
     5101 aaaatgattc taacaacagg agttgcaaaa aaaatagaag caactgaaat aggaatcata
     5161 gaaattatta tcatatttat actagattta actgaactag taaacttctt ttgattattt
     5221 tctgcactta acttagataa cataggatac ataacagata ctattgaagc tgtaaatgtc
     5281 cctgtaacaa aaccatttaa tctatctgca taagtaagtg ctggaatgct tcctttaact
     5341 aatgttgagg ctaaagttgt atctaccaat gagttcactt gattaacagc aacacctata
     5401 aatactggtg aaagtaatgc tagtaattta ataaggctat catctttaaa gtttaagtag
     5461 tataagtatt tgtagttcgt ttttttaaca aaaaacatgt aaaagagcag ttgaactacc
     5521 atagcaacca cagcaccaat tggaagtatg tatggtccaa atatggtact taacattatt
     5581 gaaataatta ttactatatt atagggtata cttccaaatc caactacaat aaagttctct
     5641 ttaatttgta aaaatgctga cataacactt gtaataccta tgaatattat tcctgttctg
     5701 aacctcccat gactaaagtc acagggttcc tgcttcatag aattttgcat actaaaatat
     5761 gtcttactaa ttctccacag gctatccccg tagttcctac ggttcttaca tacgttattt
     5821 attttgaatt gttattagtc ttaatccttc ttttaatata tttctacttg catttatatc
     5881 tctatcgtga gtttcattac aactaggaca aatccattct cttatattta agttcttaac
     5941 ttcctcgttt ttatacccac atttattgca tatttgtgaa cttgcaaaga atttacctac
     6001 ctttacaatt tgtctaccat gccaattagc cttgtattct aattgacgaa taaactctga
     6061 ccatgataca tcagaaatta agcgagatag tttatgattt ttaatcatat tctttacttg
     6121 taaatcttct atgcaaataa tatcattctc ttttattaat ttagtagtta atttttgtag
     6181 aaaatcattt ctttgattag ctatatgttc ttgaagtctt gcaactttta atcttgcttt
     6241 atttctattt aaactaccga ttgtttttct cgataattct ctttgtaatt tagcaagttt
     6301 gtttaatgac ttcttaaaat acttaggatt ttctataaat tctccacaac ttgagataca
     6361 aaattcttta attcctaaat ctaaacctat ctgattgtta gtattttcaa at
//
LOCUS       DAMJVI010000155         6374 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.160, whole genome shotgun sequence.
ACCESSION   DAMJVI010000155 DAMJVI010000000
VERSION     DAMJVI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.160"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            294..1238
                     /locus_tag="PC545_003436"
     CDS             294..1238
                     /locus_tag="PC545_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HDF2458153.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFTTINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGEASVEADREVVKLALKILEKYNNNFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNTHELKVYIEELKIKREVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1260..1886
                     /locus_tag="PC545_003437"
     CDS             1260..1886
                     /locus_tag="PC545_003437"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDF2458154.1"
                     /translation="MDYLTIALAKGRIEGESFKKFKKMGLGDSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            1920..2969
                     /gene="hisC"
                     /locus_tag="PC545_003438"
     CDS             1920..2969
                     /gene="hisC"
                     /locus_tag="PC545_003438"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDF2458155.1"
                     /translation="MREKESIRELKGYEPNHVNCKVKLDANEGSKRLFKYLIKEISDS
                     DIDLNLYPEDSYSDLKESIIDYINISGVNKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVINIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKREEIIKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEEREVLYK
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     SMFVGAIRDILKKEK"
     gene            2972..3550
                     /gene="hisB"
                     /locus_tag="PC545_003439"
     CDS             2972..3550
                     /gene="hisB"
                     /locus_tag="PC545_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HDF2458156.1"
                     /translation="MRIWKVERNTLETQILVELNIDGSGKAEIDTGIGFLDHMLTLMS
                     FHGKFDLKVICKGDTYVDDHHSVEDIGIAIGEAFKNALGDKKGIRRYSNIYIPMDESL
                     SMVAIDISNRPYLVFNAKFDTQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3552..4157
                     /gene="hisH"
                     /locus_tag="PC545_003440"
     CDS             3552..4157
                     /gene="hisH"
                     /locus_tag="PC545_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDF2458157.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSDIDEIKQADSLILP
                     GVGAFRDSISALDKLGLIPIIKEHVSKGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELIAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4154..4876
                     /gene="hisA"
                     /locus_tag="PC545_003441"
     CDS             4154..4876
                     /gene="hisA"
                     /locus_tag="PC545_003441"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDF2458158.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKVNVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIEDIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAIENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEVVSNVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFDIYERIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4858..5616
                     /gene="hisF"
                     /locus_tag="PC545_003442"
     CDS             4858..5616
                     /gene="hisF"
                     /locus_tag="PC545_003442"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDF2458159.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERKTSLEFVTKVAENINIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKKEDFYEAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKDMGVEVRL"
     gene            5644..6312
                     /locus_tag="PC545_003443"
     CDS             5644..6312
                     /locus_tag="PC545_003443"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDF2458160.1"
                     /translation="MDNKCNNVYSDEVEKFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKDAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDSSKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2605 a          540 c         1258 g         1971 t
ORIGIN      
        1 tgtaaattta gaatattaat aaaaattatt tgggaggaaa ttatcatggt gagattaaat
       61 atagtaaaat taaatacaaa attaaatcat caccatcatc atagattaaa gggattgtaa
      121 tatgaaatta attcatagac acaagccctg tttttagtgt aattggtctt gtatctatga
      181 tgggaagtaa ttttaagaga aaattatagc catataggta taaagcctat gtggcttttt
      241 tattgtttta aatttgcaaa tataagagta aatttaatga agagggtgag aatgtgaaat
      301 ttttgaggat tgaagatgag gttatttatt ctaaaaaaag atataaatta aaaagggaaa
      361 tagataaact ttttttggat gaagaatatt ttcaaataga accacaatta tttgaagaat
      421 atgatgaatt tacaactata aataataaaa ttccagaaga atctatggta aaggttgtaa
      481 atggaaaagt aatggtactt agagctgata taactacaag tataataaaa agccttgtac
      541 caagatggga agatggttta aagcttaagc ttttttataa ttcatcaata tataaaaata
      601 aaaatacagt tggaataaaa gagataagac aaataggttg tgagtatcta ggagaagcct
      661 ctgtagaagc agatagagaa gtagtgaaat tagctcttaa gatacttgaa aaatacaata
      721 ataattttat tctagaggtt gggagtagca agtatattca tggtttgtta gaagaattaa
      781 atttaaataa gaattgtgaa aatcaaatta aaaatttact atatacgaag aacactcatg
      841 aactaaaagt atatattgaa gaattaaaaa ttaagcgtga agtaaaagag ttattatcaa
      901 atattttaag tttacaaggg aatttatata atgttattga aaaatcaaac aagttctatt
      961 gtaataataa gatgaagcaa gctcttgaag aattaaagca agtaaataac ttgattgagg
     1021 aatgtaattt tttagataag gctagatttg atttatcaat gattactatg cttgactatt
     1081 atgaaggaat tatgtttaga ggatactacc caaactcata taaagaaatt cttagtggtg
     1141 gaagatatga ttctttaact aaagagtatg gaaaagaaat tccagccatt ggatttacac
     1201 taagtgttga tgagttaatg aagtatgtat ataagtaaag tttagaagga gggattgctt
     1261 tggattattt gactatagca ctagcaaaag gacgaattga aggggaatca tttaaaaaat
     1321 ttaaaaaaat gggtctagga gacagtatag acacagatac gagaaaacta atttttaaag
     1381 atgaagaaaa taaaattata tatatacatg taaagccatc agatgtagtt acatatgttg
     1441 aaaaaggagt cgctgacctc ggtatagcag gtaaagatac tattttagag aatgagacag
     1501 atgtatatga aatatatgat ttaggttttg gaaaatgtaa atttgctgta gcaggattaa
     1561 agggagatag catatataga gaagatgaat atttaaaagt ggcaacaaag tatcccaata
     1621 tagcaaaaaa gtattttaaa gagaaaggtc aaaaaattga aataataaaa ttaaatggtt
     1681 ctgtagaact agctccaata gtagggttgt cagatgtaat tgtagatata gttgagactg
     1741 gcaatacact taaagcaaat ggtcttgaaa tattagaaga catatgtaat ataagtgcta
     1801 gaattatatc aaatagagct agctatagat ttaaatatga acaaattcag aatataattc
     1861 gtttatttga agaacttgat aattaaaata taattgagct ttataaggag ggatttaagt
     1921 tgagagaaaa agaaagcata agagagttaa agggatatga accaaatcac gtaaactgta
     1981 aagtaaaact tgatgcaaat gaaggaagta agaggttgtt taaatacctt attaaagaaa
     2041 tttcagatag tgatatagat ttaaatttat atcctgaaga ttcttatagt gatttgaaag
     2101 aatctataat agattacata aatatttctg gagtaaataa gaaaaatctt ctagtaggaa
     2161 acggttcaag tgaaataatt gatttaataa tacatacttt tgtagataaa gatgaagtaa
     2221 tattgagttt ctctccaagt tttagtatgt attctatata tagtcagata aatgggtcta
     2281 aatttatagg tgttgaaagt gatgaaaatt tagttataaa tatagataat gtgattgaaa
     2341 aagtaaagga gaataatcct aaaatagtaa ttgtatgtaa tcctaataat ccaactggaa
     2401 caatattaaa gagggaagag ataataaagc ttttagattc aactaatagt ctagtggttt
     2461 tagatgaagc atatatggat tttggagaag aaagcatgtt aagtgatgtt tttaagtatg
     2521 ataatctaat agttcttaga actttatcaa aggcatttgg attggctgga ataagaacag
     2581 gatatatgtt atctaatagt agtttaataa attctgttga gaaagtaaga cctccatata
     2641 atttaaattc attatcagac tttattgcta caagagcact tagaaataaa gatgtggtaa
     2701 aggcttatat aaaggaagta aaagaggaaa gagaagtttt atataaagaa atgattggta
     2761 tgggaattaa agcatataaa tcacaagcaa attttatatt attttattct gaaatcgaaa
     2821 atttatcaca aaaacttatt gatagaggtg ttttaataag aaaatttggt ggaaagttag
     2881 aaaattatta cagagttaca attggtgata aagaagaaaa ttctatgttt gttggagcta
     2941 taagagatat tttgaaaaag gagaaatagg tatgagaatt tggaaggtag aaagaaatac
     3001 tcttgaaaca cagattttag tagaattaaa tattgatggt tctggtaaag ctgaaataga
     3061 tactggaata ggatttttag accatatgct taccttaatg tcatttcatg gcaagttcga
     3121 tttaaaagtg atttgtaagg gtgatacata tgtagatgac caccatagtg tagaagatat
     3181 aggtatagct ataggtgaag catttaaaaa tgctctaggt gacaaaaaag gaatacgaag
     3241 atattctaat atctatatac ctatggatga atctctatca atggtagcaa tagatataag
     3301 caatagacct tatcttgtat ttaatgctaa atttgatacc caaatgattg gtagtatgag
     3361 tactcaatgc tttaaagaat tttttagagc ttttgtaaat gaatcgagag taacattaca
     3421 tatcaatctc ttatatgggg aaaatgacca tcataagata gaaagtattt ttaaagcatt
     3481 tgcaagagct ttaaaagagg gtagtgaaat agtttctaat gagatagcat cttcaaaggg
     3541 ggttttgtag aatgaatata atagtagact atggtcttgg aaatatagat tctgtgtcta
     3601 ggggttttag aaaagctgga atagaaacta aaatttctag tgatatagat gaaataaaac
     3661 aggcagattc acttatactt ccaggtgttg gagcttttag agattctata agtgccttag
     3721 ataaactagg attgatacca ataataaagg agcatgtttc taagggtaaa tttatgatag
     3781 gtatatgtct aggaatgcaa ttactttatg aaaaaagtta tgagtatgga gaatatgaag
     3841 gattaggact tataaaagga agtattgata aactggatat aagtttaaaa gtgccacata
     3901 tgggatggaa taatctaaaa tttaataagg caaatgatga tatacttaaa tatataaatg
     3961 aagatgatta tgtatacttc gttcactcat attatgcaaa ttcatcaaat gaagaactaa
     4021 ttgcattttc agaatatgaa aagaaaatcc ctgctattgt tagaaaaggc aatgtatatg
     4081 gtatacaatt tcatcctgaa aaaagtggag aagtagggtt aaatatatta agagcatatg
     4141 gggagatgat aaaatgataa tatttccagc aatagatata aaagataata agtgtgtaag
     4201 acttacacaa ggagaatttg acaaagtaaa tgtatattat gataatccat tagaagtagc
     4261 atataaatgg aaaaatgaag gagcagaata tatacatata gtagatttaa atggtgctag
     4321 aagtgaattt ggtgtaaata caaaaataat tgaggacata gcaaacaata tagatatacc
     4381 tattcaagtt ggcggtggag ttagggataa ggaaaaagtc aaaagtttaa taaatgctgg
     4441 tgttactaga gtaatattag gtagtatagc gattgaaaat ttgaatttag ttgaagaatt
     4501 ggtcaatgaa tataaagaga aaattgtagt ttctatagat gctaaagatg gaaaagtagc
     4561 agttagggga tgggaagttg taagcaatgt agactcatta acattatgca aacaacttga
     4621 aaagataggt gtacaaacta ttgtttatac agatatatca aaagatggga tgttgcaagg
     4681 tccaaatttt gacatatatg aaagaatagc aaaggaaaca tcactaaatg ttattgcttc
     4741 tggaggcgta acttctatag aagacgtaaa aagactaaaa gctatgaact tatatggtgc
     4801 aataataggt aaagcgcttt atgataaaaa gatagacttt aaggaggcac aacaattatg
     4861 cttactagga gaataatacc ttgtctagat gtgagaaatg gaagagtcgt aaagggaaaa
     4921 aaatttaaag atatagtaga tgttgatagt ccagaagtac ttggaaaatt ttatagtgat
     4981 tgtggtgcag atgaacttgt attctatgat ataactgcat caaatgaaga gagaaagaca
     5041 tctttagaat ttgtgacaaa agttgcagaa aatataaata taccattttg tgttggtgga
     5101 ggagtaaata aattagaaga tttcactgat attttaagaa aaggtgctga taaagtttct
     5161 ataaattcat cagcagtcaa aaatcctgaa ttaataaggg aagcttcatt aaaatttgga
     5221 gcacaatgtg ttgttttatc gatagatgca aagaaaaatg aagagggttc atggagcgta
     5281 tacgtaaaag gtggaagaga aaaaactaat ctggatgcaa ttgaatgggc tgttaaaggt
     5341 gtagaacttg gagcaggaga aatcgttgta aatagtatgg atgaagatgg aatgaaaaat
     5401 ggatatgata tagaattatt atcaaagata acttctttag taaatgtacc tgtcatagca
     5461 tctggaggcg ctggaaaaaa ggaagatttt tatgaggcgg ttaataaatc caatgtagat
     5521 ggaattttag cagcatctgt ttttcatttt ggcgagataa aaataaacga tttaaagaaa
     5581 tatttaaaag acatgggtgt agaagtaaga ttataagtta aatactaaaa ggagattaga
     5641 aatatggata ataagtgtaa caatgtatac agtgatgaag tagagaaatt tataagaagt
     5701 ataaagtttg atgataaagg gttagtccct gttgttgttc aagaggtagt tagcaaagat
     5761 gtactgatgt tagcatatat gaataaagat gctataaaga aaaccttaaa agataaagta
     5821 gcttgctatt ttagtagaag tagacaagaa ctttgggtta aaggtgagac atctggtaat
     5881 actcaaaaag tagttaagat gtcttatgat tgtgatgtag atactatact actttttgtt
     5941 gaacaaactg gtgtagcttg tcatacaggc aattattctt gtttttacag agacttgttt
     6001 gatgatagtt caaaaatgga gcttgaagta caaacaaata tattgaaaga attatatgat
     6061 ttaatcaatg aaagaaaaaa taatccagtt gaaggttctt atactaatta tctttttgaa
     6121 aaaggaatag ataagattct taaaaaagta ggtgaagaga gtagtgaggt aattatagca
     6181 tcaaaaaata ccgataaaag tgaacttatt tacgaaatta gcgatttagt atatcataca
     6241 ttggttttaa tgatagaaaa aggtgttgaa attgatgaaa taaaaaaaga attacttaaa
     6301 agaagaaaat agaataagat agattaatgg gtaataagaa aatataccac ttttaataaa
     6361 aagtggtata tttt
//
LOCUS       DAMJVI010000156         6360 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.161, whole genome shotgun sequence.
ACCESSION   DAMJVI010000156 DAMJVI010000000
VERSION     DAMJVI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.161"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            319..1341
                     /locus_tag="PC545_003444"
     CDS             319..1341
                     /locus_tag="PC545_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2458161.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSLL"
     gene            1401..2408
                     /gene="gap"
                     /locus_tag="PC545_003445"
     CDS             1401..2408
                     /gene="gap"
                     /locus_tag="PC545_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDF2458162.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2630..3832
                     /locus_tag="PC545_003446"
     CDS             2630..3832
                     /locus_tag="PC545_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDF2458163.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEKMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEDYMGLDMGPKTIANFVKTIKESKTVVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3858..4601
                     /locus_tag="PC545_003447"
     CDS             3858..4601
                     /locus_tag="PC545_003447"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDF2458164.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCKVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4610..6142
                     /locus_tag="PC545_003448"
     CDS             4610..6142
                     /locus_tag="PC545_003448"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDF2458165.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKANSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAIKAVETVDTCVGRLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYESAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
BASE COUNT         2556 a          728 c         1270 g         1806 t
ORIGIN      
        1 atcttaacta gataaaagta aacttagttg tagataaaaa taactagaaa taaaagtaac
       61 tagaaataaa attatttata gtaataaata ttagaatgaa aatataaaaa aatatatata
      121 aaaagcctta tataaaaaga aaaaatactg tgatttgaaa atggtggaga aatattttat
      181 aaaataaaaa aataaaaaag tttttgaaaa actattgaaa tctatacgca aatcctataa
      241 aataaaaata tcttgtggga cttaaataaa cactagggac gaaaaaagtc ccgaaaggaa
      301 agcaataagg agtatgttat gaagaactta ttagaaattc aaaagaaatt gattccacag
      361 gcaattgaac ttatggaaag aagatactct attttaagac aaatatcttt aagtgaacca
      421 ataggtagga gaacattatc aaatgtatta gagattagtg aaagaataat caggtcagaa
      481 actgagtttt taaaagaaca aggacttata gacgtagctg tatccgggat gactatcact
      541 aaagaaggtt ctgatttgtt agataaactt aaggacatca tgagtgacat catgggattg
      601 tctaagttac aagatagagt cagagaaaag cttggaataa aaagggtaat attagttcct
      661 ggaagctgtg atgaaaatgg aagtttacta aaagatgtaa ctagatatgg atgcgagtat
      721 ttcttaggta ttttaaaaga tggagatatc gtttcaataa caggaggaag cactatgttg
      781 gagttttcaa acgttattaa aactgataag agatacccaa actctacaat agttccagct
      841 agaggaagta tgggaactga tgtagagatg caatctaata atatagttgc ttcgataagt
      901 aagaaattac attctaattg caaactttta catataccag atgagttgga tgaaagtgca
      961 atgaaaactc ttagccaagt gccagagatt aagacaactt tggagtatat taaaagaaca
     1021 gacattttag tttttagtat aggtagagca gatgtcatgg caaagagaag aaaacttcca
     1081 gaagacaagg ttgatgagat attatctaaa aatgcagttg gagaagcatt tggatattat
     1141 ttcaacaaag atggagaaat agtttacaaa ctgaacactg ttggaattga tttagaaaca
     1201 gttaaacaag taaaagaaac tattgcaatc tttgcaggta ctgaaaaagt agaagcctta
     1261 ttagctatat caaatatgca taaaaacatg gttcttgtaa cagatgaaga aagtgcttat
     1321 aagatattgt cattattata aaagtcaaga cctagcacag agctagaaaa catacattta
     1381 ctttatagga ggtagcgaaa atggttaaag tagctataaa tggatttgga agaataggaa
     1441 gattagcatt aagaaaaatg atggagcaac aagataagtt cgaagttgtt gcaataaacg
     1501 acttaacaga cgctaaaatg ttagctcatt tatttaaata tgatacagct caaggaagat
     1561 ttaacggaga aatagaagtt aaagaaggtg cttttgtagt aaacggaaaa gaaataaaag
     1621 taacagcaga aagaaatcct gctgacctac catgggctga attaggagta gatatagtat
     1681 tagaatgtac aggattcttt acatctaaag ataaagctga agctcatata caagcagggg
     1741 ctaagaaagt tgttatatct gcaccagcta ctggagattt aaaaacaata gtattcaaca
     1801 caaacagtga tatattagat ggttctgaaa cagttatatc aggagcttct tgtactacta
     1861 actgtttagc tccaatggct aaagtattaa atgataaata cggtatagaa aaaggattaa
     1921 tgactactat acatgcatac actaatgacc aaaatacttt agatggtcct catccaaaag
     1981 gtgatttaag aagagctaga gctgctgctg gaaacatagt tccaaacact acaggagctg
     2041 caaaagcaat aggcttagta ataccttcat taaaaggaaa attagatggg gcagcacaaa
     2101 gagtaccagt tgtaactggt tcaataactg aattagtttg tacattagga aaaaatgtta
     2161 cagttgaaga aataaatgca gctatgaaag aagcttcaaa cgaatctttc ggatatactg
     2221 aagaaatgtt agtatcttct gacataatcg gaataagcta tggttcatta tttgacgcta
     2281 ctcaaacaaa agttatggaa gtagatggaa aacaattagt taaagttgtt tcttggtatg
     2341 ataatgaaat gtcttatact tctcaattaa taagaacttt aggatacttt gcacaattag
     2401 caaaatagtc agttttgaga aaatagaaaa gtagattgac aaagtccgag tttgtagttt
     2461 aatactacgg actcaggact ttttttaaga aaaaatatat aaaatacacc taaataatgt
     2521 tacatactat atataaaaat atgataatat gatatattta atagggtaaa agtatgtact
     2581 atattatact tggcaataaa aaattacttt attaaaagga gagatattta tgtcaatgct
     2641 taacaaaaaa actatagaag atattgatgt gtgcggaaaa aaagtattag taagatgtga
     2701 ctttaatgtg ccattacaag atggtgtaat tactgatgaa aatagattga acggagcttt
     2761 accaactatt caatatttaa tttctaaagg tgcgaaagtt atattatgct ctcacttagg
     2821 aaagccaaaa ggagaagcta agccagaatt atctttagca ccagtagcaa agagattatc
     2881 tgagatgtta ggaaaagaag ttgtatttgc agcagacgat aatgttgtag gagaaaatgc
     2941 aaagaaagca actgaaaaaa tggaaaacgg agatgtagta ttattagaaa atactagata
     3001 tagaaaagaa gaaactaaaa acgaagagaa tttctctaaa gaattagcat cacttgctga
     3061 aatatttgta aatgatgcgt ttggaactgc tcatagagca cattgctcaa cagttggtgc
     3121 aggtgagttc ttacaagaga gagtttgtgg atacttaatt caaaaagaat taaaattctt
     3181 aggagaagct gttgcaaacc ctgtaagacc atttacagct atacttggag gagctaaagt
     3241 ttctgataaa ttagctgtta taaacgaatt attagaaaaa gttgataatc taataatagg
     3301 tggaggaatg gcttatacat tcttaaaagc tcagggttat gaagtaggaa cttctttact
     3361 tgaaatagat aaagttgaat atgctaaaga aatgatggaa aaagctaaga ataagggtgt
     3421 taacttatta ttaccagtag acgttgttat ggcagaccac tttgcaccag atgctactcc
     3481 aatagtaaca gaagatgcta atgtaaaaga agattacatg ggactagata tgggacctaa
     3541 gactatagca aactttgtaa aaactataaa agaatcaaaa actgtagtat ggaatgggcc
     3601 aatgggagta tttgaatttg aaaactttgc aaatggtaca ttatcagtag ctagagctat
     3661 ggcagagttg actgatgcaa ctactgtaat tggcggtgga gatagtgcag cagcagttaa
     3721 tcaattagga tttggtgaca agatgactca cgtatcaaca ggtggaggag catcactaga
     3781 atttttagaa ggtaaagaat taccaggtat tgcagcatta gataataaat aaactaatat
     3841 atagaggaga ttgtaaaatg agaaaaccta taattgcagg aaactggaaa atgcataaaa
     3901 caataaaaga agctttagaa tttgtaaatg aagttaaaga taaagtaaat tctgataaag
     3961 tagaagctgt tatatgtgca ccatttactt tattaaagga tttaaaagaa gctactaagg
     4021 gtacaaatat aaagataggt gctcaaaata tgcactttga agaaaaagga gcatttacag
     4081 gagaagtttc acctcttatg ttaaaagaaa tagatatgga ctatgttgta ataggacatt
     4141 cagaaagaag acaatacttc aatgaaactg atgaaactgt aaacaagaaa gttttaaaag
     4201 cattagaagt aggaatagac ccaatattat gtgttggaga aactttagaa caaagagaag
     4261 ctggaaaaac taaagatgtt tgtaaagttc aagttgaaaa agctttagaa aatgttctta
     4321 aagatgattt agcaaaagta gttgttgcat atgagccaat ctgggctatt ggaactggta
     4381 aaactgcaac tgctgaagat gctaatgacg ttatatctta tataagagaa gttataaaag
     4441 gattatatgg agaattagct aatgaagtta gaatacaata cggtggaagt gttaaacctt
     4501 caaatgtagc cgaaataatg ggtcaaagtg atatagatgg agctttagta ggtggagcta
     4561 gtttagctag taatgattac ctagaccttg ttaatttcta aggagtgaaa tgatgaaaaa
     4621 accagttgct ttaattatta tggacgggtt tggatacaat aaggatgtta agggaaatgc
     4681 aattgctgag tccaaaactc ctaatttaga tagaataaaa aaagagtatc caaacacatt
     4741 gataaatgct agtggtcttg atgttggtct acctgatggt caaatgggaa attcagaagt
     4801 tggacacact aacataggtg caggtagaat agtatatcaa gatttaacta gaataacaaa
     4861 atctataaaa gatggtgact tttttactaa taaagtttta tgtgaagcaa tggataatgc
     4921 aaaagaaaat tctcttcatg taatgggact tttgtctgat ggaggagtac attcacatat
     4981 agaccatctt aaggctataa tcaaaatggc aaaagataaa ggcgtacaaa aggtgtatgt
     5041 tcacgcattt actgatggta gagatactga ccctcaaagt gcattagaat atgccaaaga
     5101 agtacaagct agtatggatg aaattggagt aggtgaattt gctacagttt ctggtagata
     5161 ctacgctatg gatagagata agagatggga aagagtagaa cttgcataca atgccatggt
     5221 tagaggtatt ggtgaaaaag caaattctat tgaggaagct attcaaaact cttatgatga
     5281 tggaaaaaat gatgagttta taatgccaac agttattatg aaagatgata aaccagttgg
     5341 aagtataaag gaaaatgatt caattatatt cttcaacttt agaccagata gagctagaca
     5401 aataactaga gctttagttt gtgaagagtt tgatggattt aaaagagaag atattaaaaa
     5461 cttctttgta tgcttaacag agtacgatat aactatagag aatgtgcata tagcatttgg
     5521 acctcaatct ttagccaaca ctctaggtga gtatctagct aaaaatggaa aaactcaact
     5581 tagagctgct gaaactgaaa agtatgcaca tgtaactttc ttcttcaatg gtggagtaga
     5641 agagccaaat aaaggtgaag aaagactatt gataccttct ccaaaggttg caacttatga
     5701 tttaaaacca gaaatgtcag catatgaatt gacagataaa gctttagata aattaggaga
     5761 agataagttt gactttatag tattaaactt tgcaaatcct gatatggtag gtcatacagg
     5821 aagtatagaa gcggctataa aggctgttga aactgtagat acttgtgtag gaagattgat
     5881 agataaaata gtagagttag gtggaagtgc aataataact gctgaccacg gaaatgctga
     5941 atatatgtta gacccagaaa caggaaaaac tgtaacggct cactctatta atccagtacc
     6001 atttattgta gttggtcaag aatatgaaag tgcaaaatta ttggatggag gaagattatc
     6061 tgatatagca ccgactgttt tagatatgat gaagttagaa aagccagaag aaatgactgg
     6121 tcattcactt atttcaaaat agtcgattct gataagtaga gaataatata taaactacaa
     6181 tataaattta aaaataatgc agtttaggtt tattttgtaa agtttagact ttatgacaga
     6241 agatttagat tttgtaagtt ttggatattg taaggtgtag atgaagatat attttataaa
     6301 atttacaaga tagacaattt ataaaataaa aaattataat atagataata atgtaaataa
//
LOCUS       DAMJVI010000157         6354 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.162, whole genome shotgun sequence.
ACCESSION   DAMJVI010000157 DAMJVI010000000
VERSION     DAMJVI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.162"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            151..3330
                     /locus_tag="PC545_003449"
     CDS             151..3330
                     /locus_tag="PC545_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2458166.1"
                     /translation="MKIDMIYKLIRRNTEPSRWSRGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSKGKSIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSNTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINVDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYNPNTHYFADED
                     EKLVDIIEEYGTNLSYNFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPVRFDFKNIEDKIVLYSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQILSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DGNICCDVKYIYDDEDKKFVLPNIQKEATIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSEKFKLKKIYNASSIQASINQTNQNYLEFNFNISDINPREYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMDKSKNNKIHKNKALYIN
                     DVIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGNKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPVALV
                     TESVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKMKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSMDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIINLFN"
     gene            complement(3615..4379)
                     /locus_tag="PC545_003450"
     CDS             complement(3615..4379)
                     /locus_tag="PC545_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2458167.1"
                     /translation="MAYFNYNDKQCYYNEIGDGTPLLFLHGNTASSKMFNDIIDFYKD
                     EYKVILIDFVGHGKSQMVDKFSADLWFDEAMQVICFLEAMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWEK
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDEFVSLISSDFFENTFSLLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(4535..4768)
                     /locus_tag="PC545_003451"
     CDS             complement(4535..4768)
                     /locus_tag="PC545_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458168.1"
                     /translation="MKAEDMKRKVSPIDLERILVTGGVIKHEDSIWLLDFWGNKDIAG
                     ILLMPPTRHVILHLNDCCKWKEYFRTKKKFYRS"
     gene            complement(4842..6335)
                     /locus_tag="PC545_003452"
     CDS             complement(4842..6335)
                     /locus_tag="PC545_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2458169.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLGYNIPSRVQREVIPKLLKGQNLV
                     VRSKTGSGKTASFAIPLCENINVDYNNIQALIVVPTRELALQVKDEISDIGRLKKVRC
                     SAIFGKQSIKDQIAELKQRVHIVVATPGRILDHINRGSIKLENVKYLVIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVSLFSATIDEEIKYICEKYMLDYSVINIEENESDTNQKTR
                     QIDDKIIKANGREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKNQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKGNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKG
                     ILKKVSKRKKVDKNSYNSKDIKLNIESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKYKEIPIKKKMVKVKRDIR"
BASE COUNT         2367 a          900 c          738 g         2349 t
ORIGIN      
        1 ttcaataaaa atctaatagt aaattgttat ttacacatat attgtgatag ataaaagcta
       61 ttcatatctg ccttaatttt agtcttaaat ttagagtgta aattgaatgc atttagatta
      121 taatatatac agtgaggtaa ggtgaataat atgaaaatag atatgatata taaactaata
      181 agaagaaata cagaaccatc aagatggtca agaggtgtaa aatattatac taatcaatta
      241 gttgattata tagacataga tattgatgac ctacaaaata acctgttgac atttcgtggt
      301 ggagtcaatt ctgaagataa ttatagcttt tattttaatt caataactat agatataaag
      361 cactttaaga taattgaaaa tgattgtagt tgcgctgact gtcaatcaaa gggcaaaagt
      421 atttttatat gtaagcatat agttgctatt gtgataaaaa gcttaaaaca aataaaaaat
      481 aaaaaagatg ttcctcctat tctaatagat atattaggtg cagataagga taagctttct
      541 aatacaaatt ctaaaaccag cttagattta agcaagcaat tactttcttt gttagacaat
      601 actaaaagag aaagggttaa tttagatgta ttgataaatg tggattcaaa taaagcaatt
      661 gctgaattta aaataggaat tgataaaatg tacattttaa aaaacattac tgaatttgca
      721 tattcaaaac ttaatggata tgaattagat tttgggaaga aatttactta caaccccaac
      781 actcattatt ttgctgatga agatgagaag ttagtagata ttatagaaga atatgggaca
      841 aatctttcat acaacttttc ttcatcacat tcaaaaaaat ttatggagat aaatgcaagc
      901 tctttaaaac aatttttaaa tgctattaag tacaaggatt ttacccttat tttttctaaa
      961 gaggaatatc atccaaagat attaaatgat actctacctg taaggtttga ttttaaaaat
     1021 atagaagata aaatagtctt atattctaaa gattcactac ctgttccact tactaaaaaa
     1081 aatgatgtac ttttatatga tggcaatatc tatttattat cacataaaaa agctcatgat
     1141 tattcaaaaa tatatcaaat attatcaaag actaaagagt taaaatttga taaagaggat
     1201 tctaaggatg ttttaggatt gctagttcct agattaaaat ctatatctca agaagttcat
     1261 ttagatgata acataaaaaa taatataaca aaagatttta aatctgagtt ctactttgat
     1321 atgattgatg gcaacatatg ttgtgatgta aagtatatat atgatgatga agataaaaaa
     1381 tttgtgctcc ccaacataca aaaggaagct actattgaaa ataaacttac ttcttcacaa
     1441 tttacaaagg aaaataatca ttatgtgttt aaaggaactg accatgacct atttacattt
     1501 ttagatttac aattagaatc tctaaaggaa tttggagata tatattattc tgagaagttt
     1561 aaattgaaaa aaatatataa cgcttcatca attcaagctt caatcaatca gactaaccag
     1621 aattacctag aatttaattt taatatatct gatataaatc caagagagta caaggatatt
     1681 ttgaaagctt ttcaagaaaa acgtactttt tataaactta aagatggaaa ctttatagat
     1741 ttatcagaaa gagaaacaaa agattttttt gaattagttg aaaatctaga tataatggat
     1801 aaatcaaaaa ataataaaat tcataaaaat aaagctctat atataaatga tgtaatacgt
     1861 agtaaaaatt taaagtttat agatggtaaa aaagaactag acagtatatg tgaaaaattt
     1921 agaaattttg atagcataaa tttagaaatt cccgcaaatt taaatgcaaa ccttcgtgac
     1981 tatcaaataa atggcctcaa ttggtttaaa gttttagatt attataaatt tggtggtata
     2041 ttagctgatg aaatgggtct tggtaaaaca atacagacta ttgcattttt attatcatta
     2101 tctaataaaa aaagtcttat tgttacaccg acatcactca tttataattg gaaaaatgaa
     2161 tttgaaaagt ttgctccaga tataaaagtt ttattgatac atggaaataa aagagataga
     2221 gaaaaatgtt ttatggaatt agaaaatttt gatgtaatac ttacaacata tggtactctt
     2281 agaaatgatt tagaaaaata tagtgaaata aagtttgatt attgtatatt agatgaagct
     2341 caaaatataa aaaatccagt agctttagta actgagtctg taaaaagtat aaatgcagaa
     2401 aataaatttg cactcactgg tacacctatg gaaaataatc tactagaatt atggtctata
     2461 tttgatttta taatgcctgg ctatctatat agtaaagcaa agtttcagga attgtttata
     2521 aataaagaag ataatgttaa aaacctgaaa aaattgataa aaccttttat tcttagaaga
     2581 agtaaaaaac aagttatgaa agaacttcca gataagattg aaaaaaactt ttttgttgaa
     2641 ttgaataaag agcagaaaaa gatatatagt gtatattcaa aagatataca agataaaatg
     2701 aaagataaaa atctaaagaa agataaaata gttatatttt cttatttaac taaactaaga
     2761 caactttgtt tagacccaag tatagtagta aaagattaca ataaaaaaag ttcaaaaatt
     2821 gaaacatgtt tagaaatact tagggatagt ataaacgaaa accacaaaat attattgttt
     2881 tcacaattta cttctgtgct taagaacata agtaaggaac ttgataagta taaaattaaa
     2941 taccattata tagatggtaa aactaatgcc aaagaaagac ttgaacttgt agatgagttc
     3001 aataatagta tggataaaaa agttttttta atatctttaa aagcaggcgg aactggttta
     3061 aatcttacat ctgctgacat ggtaattcac tttgacccat ggtggaatcc atcagtagaa
     3121 aatcaagcta gtgatagagc tcatagattt ggtcaaaaaa attcagtaca agttataaaa
     3181 ttgattgcta aagggactat agaagaaaaa ataataaaat tacaagaaag taaaaaagag
     3241 cttataaatc aatttataaa tggagaactt tctaatgaag gtgtattaaa aagtctatct
     3301 gatgaggaga taataaactt atttaactaa attatttaat cctacagctt aactatataa
     3361 atttgttcta acatttatat agttaagcta aaatatctga ataaataaaa tttaatattg
     3421 ttatttaatc ccaactttac aatctaatga tatctctttc tatacatatt gcatatacaa
     3481 atgtaataac ttgactatta gaatattttg ttttaacaat actatttcat cataataatg
     3541 aagtatatgt acactcactt ttgtaagttt tgcaatccat tttacagttt tcattattat
     3601 aaattttggt taaattattc ctctaaaaat ttctttgcta catttgaaaa atctaatcca
     3661 ttcgataaaa ttgcaggatg accaccttta tcaaacaaat acattttacc atttttaatc
     3721 ttttcaagaa gtaatgaaaa agtattttca aaaaaatcac tagatattag agatacaaac
     3781 tcatcttctc tacttcctgt taaaagcacc tctggttgca tagtttctag aggcttatga
     3841 aaaaattttc ctatagtttt ataatgttca aaaatagctt ctgtatcatt atctacaact
     3901 ttttcccagt tttctccttg attataaata taaaaagctt ttgcaccatc atcttgctta
     3961 gatgcttcac gctctatttt cacattttgt acaaatgact ctagaggaac ttctccttca
     4021 aaactatctg caattacctt attcactaaa tcaggtctct caagtgcaac atttaatgca
     4081 acaagagctc ctccactact accaattatg tttacttttt tatagttcat tgcttctaaa
     4141 aaacatatca cttgcatagc ctcatcaaac cataagtcag cagaaaattt atcgaccatt
     4201 tgagattttc catgacctac aaaatcaatt aatattactt tatattcatc tttataaaaa
     4261 tcgattatat cattaaacat cttggaagag gctgtatttc catgtaaaaa aagtaatggt
     4321 gtaccatccc caatttcatt atagtagcac tgtttgtcgt tatagttaaa atatgccata
     4381 tatttccccc ctttaatatc cctataggta tatatttatt acttatattt aaaatgacta
     4441 ttccattagc cacttaaaaa tagtataact tagaatgtag ataaaatcaa cattccttgc
     4501 taatttatct attgtttgtc taatttatta taatttaact tctgtaaaat ttcttttttg
     4561 tacgaaaata ttccttccat ttacaacaat cattcaaatg aagtataaca tgacgagtag
     4621 gaggcataag caaaattcca gcaatatctt tattccccca aaagtccaac aaccaaatag
     4681 aatcttcatg ctttattact cctccagtca ccaaaatcct ttctaagtca attggagaga
     4741 cctttcgttt catatcttca gctttcaaat ccttaacaac ttctattgtg ttttttgcaa
     4801 cttcatttcg ataacaaagc aattcatcaa tattgactaa attatcgtat atctcttttc
     4861 actttaacca tttttttctt tataggtatc tccttatatt tttttaatat caaatctccc
     4921 ttataattta atatatctac atatgaacat aaatcttgaa cttcaattac acctatatca
     4981 tcattagtta taccttttat attactaaat gcaccaacta tatctaaaac tcttattttc
     5041 ttcttcttac cagcatttaa atataattta acaacttcac tttctatatt tagttttata
     5101 tctttactat tgtaagaatt tttatcaact ttttttctct ttgatacttt ttttaggatt
     5161 ccttttgaat attcttcaaa caaaattttg ccatgtgtaa ttctactttt ttctatatct
     5221 tttaattcat ttattttata tcctatatac gtttctatat tctctatata tttttcatcc
     5281 ttttcagtta ctatagtaat tgcctttcca ctatttcctt ttctcccagt tctacctatt
     5341 ctatgtatat agttttcttt atcttgtggc acatcataat ttattactaa agaaatatca
     5401 tcaatatgta ttcctctgga cgctacatca ctactaacta atacattaaa tttttggttt
     5461 ttaaaatcct taattacaaa aattcttctt tcttgtgata aatctgcatg taactctctt
     5521 attaaaaaac cttctttact catcttgtta tataatttag atactttctc tttggtatta
     5581 caaaatatta tcacactctt tggattttcg ctgtatatta attcttttaa aaggatgtac
     5641 ttctctcttc catttgcctt gattattttg tcatctattt gtcttgtttt ttgattagta
     5701 tcgctctcat tttcttctat atttataaca ctgtaatcta gcatatattt ttcacatata
     5761 tattttatct cttcatctat tgtagctgaa aaaagactta ctattttctc cttaggtaaa
     5821 tttaataata ttttttccat ttgttcaaca aaacctttat taaacatttt atctgcctca
     5881 tctattacta aatactttac attttccaac ttaatagaac ctctatttat atggtcaagt
     5941 attctgcctg gagtagctac aacaatatga accctttgtt tcaattcagc tatttggtct
     6001 tttatagatt gtttaccaaa tatagcacta catctcacct tctttagtct ccctatatct
     6061 gatatctcat cttttacttg caaggctaat tctcttgttg gaaccactat aagagcttga
     6121 atattattgt aatctacatt tatattctcg cataaaggta ttgcaaaact tgctgtcttt
     6181 ccacttcctg tttttgacct aactactaag ttttgacctt ttaataattt tggaataact
     6241 tctctttgta ctctagaagg tatattatat cctaaactct tcaatgactt aagtatcttt
     6301 tcatttaact taaatttttc aaatgtatat ttcatatatt accaccttat tata
//
LOCUS       DAMJVI010000158         6025 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.163, whole genome shotgun sequence.
ACCESSION   DAMJVI010000158 DAMJVI010000000
VERSION     DAMJVI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.163"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            174..461
                     /locus_tag="PC545_003453"
     CDS             174..461
                     /locus_tag="PC545_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458170.1"
                     /translation="MKKILYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFNYLTS"
     gene            complement(1000..1551)
                     /locus_tag="PC545_003454"
     CDS             complement(1000..1551)
                     /locus_tag="PC545_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458171.1"
                     /translation="MSINTVIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SIHTYHNQGEKTLKAVIINYYPV"
     gene            1742..2704
                     /locus_tag="PC545_003455"
     CDS             1742..2704
                     /locus_tag="PC545_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDF2458172.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIADEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQKIAKERNAFRADQFNLEA
                     CNHAHEKYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKTLKKHNSSIKCYVVEPETA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQVTDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSSEKGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            2999..4843
                     /locus_tag="PC545_003456"
     CDS             2999..4843
                     /locus_tag="PC545_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDF2458173.1"
                     /translation="MQKRILRNFIFTGKNILSKLVFIIKYNFSTLILFELFHKGLALF
                     FIWPSIRYIFDILIKSLHIVYLNMDNFIKVLTNPMSIVLIILSVIILAFYAFFEFTSV
                     IICFNKSIKYEKIGLFELFKISFENSIKLLYPKNILLFIFVILIIPLVNTVLISGFIG
                     KIQIPEYILDYIKSDIILNIIYMIFLLIIYISVIRWIFSIHEITLNTENFKRARKESV
                     NLIKGKIIRTIIYSLILSLVVAIIGYAIYHSGIIFIGIWTKYCTDMEFLKNVFINRCI
                     AFEEYFRLILSIFIFILDIGFISATYYQYKGVNISKNNIKKEKKSISKIVFNLVLILL
                     ISYIESVAFSSHINGMFNTSFSFYTTAIAHRAGAEMAPENTLAAIEEASHAKADYAEI
                     DVQETKDGELIVLHDSNFKRTTGVDKNVWDVNYNEVKTYDAGSFYYYGKGYYDEKFVG
                     EKIPTLKEMIKYSKGKVKLLIEIKLNGHEKGDVVKKVVQLIKENNFENQCIVASMDKT
                     ILQKVKKLDENLLTCYFMAIAYGDFSKLNYVDIYGIESTFVNKTVVEKIHKMNKKIFV
                     WTVNSDSLIEKMLDLNVDSIITDNPYLIESAIYWKKNGFISQVSDYLF"
     gene            5165..5749
                     /locus_tag="PC545_003457"
     CDS             5165..5749
                     /locus_tag="PC545_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDF2458174.1"
                     /translation="MTEREKMLAGELYDCGDIELLTQWHKAKDLVRDYNQTDSLDLEK
                     KEKILNELLGGKGKNLWITAPYYVDYGNNIYFGNNCEVNMNCTFLDDNKIIIGDNALI
                     APNVQIYTAFHPTNAGDRFGNAKEDGSFEFCKTQTAPVTIGDNVWIGGGAIIMPGVTI
                     GDNVVIGAGSIVTKDIPSNMIAYGNPCRIIRENK"
BASE COUNT         2352 a          585 c          930 g         2158 t
ORIGIN      
        1 tgaaatttat gaatattcat actataattt aaatataagg agatgccctt tgaaaataaa
       61 aattaaaaaa tatttaatgc tactacaata ggaactagaa ctacacttaa taaatataca
      121 gaaattagat ttggctcaat ataaaataca aatatagaat ttaggtgttt tttatgaaaa
      181 aaattttata tgctttatat agtttcattg ttattatagc taattttaga ttaaaagaaa
      241 aaaattataa ctttatttta ttagcaatct tttttatatt gctgttaatt caaaatggag
      301 ttttaaaaac aggttatcaa aatagagcta cattttatca agataattat tttaatggag
      361 aggataaaaa tgataagata aaatttatga ctataaagat ttttatattt ttgtcattac
      421 cattatgttt taatatacta ttcaattatt taacctctta aattatattt agatatagga
      481 gtcttatgat aaaagtaagc ttatgtgtta tttgtagtaa atataaagtg catatagtga
      541 taaaacaaaa gtttcatata aatgaagaaa aaaatataaa ataatttatt taactactta
      601 aaaataaact aaaagggata ataaaatagc ttttagttta tggtaataga ggtatttaaa
      661 aagagtgcat aagcactcca attgtgagaa atacatacac aaatcattat tacaacaatg
      721 aaaaatgtac atataagaga cgttttgtat ttgttttaag tgataatatt ttaatactaa
      781 ctaaataatt atcacaatgg taaaaaagaa aaaggagtat tttgtactcc atttgtcaaa
      841 aatataaaac ttttatataa gcttattata acataattga taaaagtgta aagatatata
      901 atgaatttat aaagaaaaat aaaataaaaa agataaatcc taatcaaagg atttatcttt
      961 ttgtatagaa aataattatt ctattattaa atggcatggc taaactggat aataatttat
     1021 tattactgct ttcaatgttt tttctccttg attatggtat gtatgaattg attcagctga
     1081 aaaacaaata gagtcatttt ctctaagttg ataaatagag tcatttactt ctaattttaa
     1141 ttgaccttct ataatcataa tatattcctg tgatttttca gaatgaccaa cagatgtata
     1201 actatgacct tcttctaatt ccatttgaaa taattcgaag tttctagttg gtgtattggg
     1261 ataataacaa taaagacgat aatctttatt ttctgaaact tgtacatcta tatcagtttt
     1321 tgatacaata aaagtttcat tttgaggttg ttccagtatt gcagtatatg gaacatttag
     1381 tccacttgca attttccaaa ttgtgtttac tgttgggttt gagtctccct tttcaatttg
     1441 agaaagcatt actttactta cgccagataa ttcagcaagt tgacctagac ttaagttacg
     1501 ttcattacgt aatctattta aatttttagc aataacagta ttaatactca tagtagctcc
     1561 ttaaaatgta aattattgac aaatataatt tacgaatgta taatttattt aacattattt
     1621 ataatttaca aatgtataat ataatgaacg ttaaatacac tgtacatccg taatataaat
     1681 tatactgcat aaagagttgt ttttcaacgg atgatagttg agagattaag ggggaattgt
     1741 aatgcgtgta cttaataatg ttacagaggc tataggcaat actcatatgt tacatttatc
     1801 caaaatagca gatgaatttg gagttcaagg taatatttat gcaaagatgg aatacttaaa
     1861 tccgggattt agtaaaaagg accgtgtagc attacaaatg attaccgaag cagaaaatgc
     1921 aggtttatta gttccaggtc aagcagttgt ggaattaaca agtggtaata ctggaacagg
     1981 actagcaata gcttgtgcat gtaaaggata taaatttata gcgtgtatgt caaaaggaaa
     2041 ttctatggaa agagcaagaa tgatgaaagc tttaggtgct gaagtagttc ttgtagacca
     2101 aatgcctaat tcagttcatg gacaggtatc tggtgaagat ttggcacttg tagaaattga
     2161 ggctcaaaaa atagcaaaag aaagaaatgc atttagagca gaccaattta atttagaagc
     2221 ttgtaatcat gcacatgaaa agtatacagc agaagaaatg tgggaacaat tggatggaaa
     2281 tattgatgta tttgtagatt ttcttggaag tggtagtact tttgcaggat gttcaaaaac
     2341 acttaaaaaa cataattctt ctatcaaatg ttatgttgtt gaacctgaaa cagcagcaat
     2401 ttattcaggt aaagaaataa ctgaccaagg gcataaaata caaggtggag gatattctat
     2461 ggatttgcca ttagtagaca agaaacttat tgatggttat attcaagtta cagacgagga
     2521 ggcaactgat gtagcaagaa aattagctaa attagaaggt gtatttgcag gattttcttc
     2581 aggggcaaat gtatgtgcag caattaagtt gttaaagagc agtgaaaaag gaaaaaatat
     2641 agttttaact cttaatgata gtggtttaaa atatttaagt actgatttat atgaagaatt
     2701 gtaataatag cacaatagtt atgcaatgtt ttttaaatta agtatataga tttaaattat
     2761 aaaaagtatc tcaatgtaaa ttttgaaagt tataaaggag atactttttt attttgtaaa
     2821 tatggagtat cctacttata agtattaagc tatatagata ttgatgatta gctatgcaat
     2881 aaaaaagagt atgtgttttt gcatttatac atcataactt ggaataatac atattaatgt
     2941 tctaaatgta tttaaaatat atgataaaat tagaacaaat agatacaagg agataaaaat
     3001 gcaaaagaga attttaagaa attttatatt tacgggtaaa aatattttaa gtaaacttgt
     3061 ttttataata aaatataatt ttagtacatt aattttattt gaattatttc ataaaggatt
     3121 agccttattt tttatatggc catctattag atatatattt gatattttaa taaaaagttt
     3181 acatatagtc tacctaaata tggataattt tataaaagtt ttgaccaatc ccatgtcaat
     3241 agttctgata atactatcag ttataatact agctttttat gcattttttg aatttacctc
     3301 tgtaattatt tgttttaata aatcgataaa atatgaaaaa atagggttat ttgaactctt
     3361 taaaatatca tttgagaact ctataaaatt gttatatcct aaaaatatac tattatttat
     3421 atttgtgata ttaataattc cattagtaaa tacagtttta atttcagggt ttataggaaa
     3481 aatacaaata ccagagtaca tattagatta tataaagtct gatataattt taaatataat
     3541 ctatatgatt tttcttttaa taatatatat ttcggttatt agatggattt ttagcattca
     3601 tgagataact ttaaatacag aaaattttaa aagagcaaga aaagaaagtg ttaatcttat
     3661 aaagggaaag attataagaa caattattta ttcattaata ttatctttag tagtagctat
     3721 tattggatat gcgatttatc atagtggaat tatatttata ggcatatgga ctaaatattg
     3781 tactgatatg gaatttttga agaatgtatt tataaataga tgtattgcat ttgaagaata
     3841 ttttagattg attttatcaa tattcatttt tatattggac attggattta tctcagcaac
     3901 ttattatcaa tataaaggag taaatatttc taaaaataat attaaaaaag agaaaaaatc
     3961 tatatcaaaa attgttttta atttagtatt aatattattg atttcttata tagagtcagt
     4021 tgcattttct tcacatataa atggtatgtt taatacgtca ttttcatttt atacaacagc
     4081 aattgctcat agagcaggag ctgaaatggc tccagagaat actttggcag caatagaaga
     4141 agcttcacat gctaaagctg attatgcaga aatagatgtt caagaaacta aagatggaga
     4201 acttattgta cttcatgatt caaactttaa gagaactaca ggagtagata aaaatgtttg
     4261 ggatgtaaat tataatgaag taaagacata tgatgcggga agcttttatt attatggaaa
     4321 aggttattat gatgaaaagt ttgttgggga aaagatacca acactcaaag aaatgataaa
     4381 atattctaaa ggtaaggtga aattattaat agaaataaaa cttaatggac atgaaaaggg
     4441 agacgtagta aaaaaagttg ttcagttaat taaagaaaat aattttgaaa atcaatgtat
     4501 agttgcatct atggataaaa ctatattaca aaaagtaaaa aaactggatg agaatttact
     4561 gacatgctat tttatggcaa ttgcatatgg tgatttttca aagttaaatt atgttgatat
     4621 atatggaatt gaatcaactt ttgttaataa aacagtagta gaaaagattc ataaaatgaa
     4681 caaaaaaatc ttcgtttgga ctgttaatag tgacagttta attgagaaaa tgcttgacct
     4741 aaatgtagat agtattatta cagataatcc ataccttata gagagtgcaa tttattggaa
     4801 gaaaaatgga tttattagtc aagtgtcaga ttatttattt taaatattct ataattgtat
     4861 tatccagtaa aagagatatt attaaaatca ataatatctc tttttaacat aaataaggtt
     4921 gttaaattaa ggctattctt ataaaaattg tgtttatcag atataaataa ggaataaaag
     4981 taaaaaaatt gctatgacat tattacaatt aaaagagttt taatatgata gagaggtaga
     5041 ttgtcaactg tatatttaat aaattttatt atttattaga ttttatataa gtaaaatata
     5101 tagatatata aatcttatgc tagaatatat atgaaaaaca atatagattt ggagggttaa
     5161 aagcatgaca gagcgagaaa aaatgttggc aggagaactt tacgattgtg gtgatattga
     5221 gttgttaact caatggcata aagcaaaaga cctagtaaga gattataacc aaacagattc
     5281 cttggatttg gaaaaaaaag aaaaaatatt aaatgagtta cttggtggta agggtaaaaa
     5341 cttatggatt actgcaccgt attatgtaga ttatggaaat aatatttatt ttggaaataa
     5401 ttgtgaagtc aatatgaatt gtacgttttt agatgataat aaaattatca ttggggataa
     5461 tgcactaatc gcaccaaacg tacaaatata tactgcattt catccaacta atgctggtga
     5521 tagatttggt aatgccaaag aagatggttc atttgaattt tgtaaaactc aaacagctcc
     5581 agttactatt ggagataatg tttggattgg cgggggtgct attatcatgc caggtgttac
     5641 tattggagat aatgtagtta taggtgcagg aagtattgtt acaaaagata ttccaagtaa
     5701 tatgattgca tacggaaatc catgccgtat tattcgagaa aataaataaa gttaaatttt
     5761 cttgcaaaat ctcaaaaaac acctttctta ctttatacat agggtatttt ctaatacttt
     5821 cacatccata ttcatttgtt aaatttttgt ggaatgtagt ttttatcact tctatcttac
     5881 atttccacca tttttttata aaagtcattc ctttatagat ttatttataa tagtatatat
     5941 tctatttgat gtttgaataa gcacttattt ttaagtaaaa tactaacttc taggttagta
     6001 ttttactata gtatgatgat taagt
//
LOCUS       DAMJVI010000159         5984 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.164, whole genome shotgun sequence.
ACCESSION   DAMJVI010000159 DAMJVI010000000
VERSION     DAMJVI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.164"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            165..977
                     /gene="modA"
                     /locus_tag="PC545_003458"
     CDS             165..977
                     /gene="modA"
                     /locus_tag="PC545_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458175.1"
                     /translation="MFKKLSVLALAMIISLSITACNSKENKANDESNTKVTTADDSKE
                     KETLFVYSGAGLKKPMDEIAKKFEKENNVKVEYSYAGSAQLIAQIETSHKGDVFIVGS
                     EPIYSKAFEKELVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNKDGVKLILGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIESDEAKQIFKKYGFEPVK"
     gene            987..1778
                     /locus_tag="PC545_003459"
     CDS             987..1778
                     /locus_tag="PC545_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458176.1"
                     /translation="MKNKFFTTLLTGFFAIVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKIFKLDFIFTKQGIVLAQIFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFRFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFLSLTCLMLFEIFFNREVDKN"
     gene            1780..2475
                     /locus_tag="PC545_003460"
     CDS             1780..2475
                     /locus_tag="PC545_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2458177.1"
                     /translation="MLKIKNLSLDLPNFKLQENSLNINNHEYFVLLGASGSGKTLFLE
                     TLAGRYPSAKGNVSYKENLISNLPPENRNIGFVYQNFELFPNMNVEDNIRFPLKLRKI
                     PIDEQILKANHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKNKGESFFYEYI"
     gene            2462..3193
                     /locus_tag="PC545_003461"
     CDS             2462..3193
                     /locus_tag="PC545_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458178.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIVGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLHDNSHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEVCAKKFPEFIKMEFGNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTGSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            3507..4307
                     /locus_tag="PC545_003462"
     CDS             3507..4307
                     /locus_tag="PC545_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDF2458179.1"
                     /translation="MTFKNKKFTINKYKILSIILVLCLILTAFSSYVNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTNIQK
                     SEYRNREFLASLNSLGVIRENIILLPETNTNGNTDFNYMEKIALTFEEKFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEYREGIGYKSDHKSFDKLLNNKDVPAILHTPNL"
     gene            4846..5562
                     /locus_tag="PC545_003463"
     CDS             4846..5562
                     /locus_tag="PC545_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458180.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLRPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKIIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSHEIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
BASE COUNT         2331 a          728 c          844 g         2081 t
ORIGIN      
        1 aaaataacta cctatggaaa tgacattatg aatagatttt ttcttaacta tacataacaa
       61 aacataacaa aacattataa aacataacaa aaacttagac atcgttttct tagtatgata
      121 tactaaaaaa gaatttacac taatttaata agggggaata tcttatgttt aaaaaacttt
      181 cagtactagc tttagctatg attatttcat taagtattac tgcatgtaat tctaaagaaa
      241 acaaagctaa tgatgaatct aatactaaag tgacaacagc tgatgattca aaagaaaaag
      301 agactctttt tgtctattca ggggcaggtt taaagaaacc aatggatgaa atagcaaaaa
      361 aatttgagaa agaaaataat gtcaaagttg aatatagcta tgcaggttct gcacagctaa
      421 tagctcaaat tgagacaagt cacaaaggag acgtatttat tgttggttct gaaccaattt
      481 attcaaaggc ctttgaaaaa gaacttgtag gtgaacataa aacggttgca catcatacac
      541 ctgcaatagt tgtacctaaa ggaaatccag ctaatattca aaaattggaa gacttaaata
      601 aagatggagt taaacttatt ttgggagaca aagagtctaa tgctataggt aagactacac
      661 aaaaaatact tgaaaaaaat aatttaaaat ctataaatga taatgttgtc tctacagctg
      721 caactgtaaa tgaaatgatt gtacagttaa catcaagtaa agctgatgct acaatagcca
      781 caaaagattc ggtctttggt aatgaagatg tagaagttat agaaattgcc caagataaaa
      841 atatagacca actcatatca gcaggaatcg taaactatag tgataaaaaa gatttagcta
      901 ataaatttat aaattatata gagtcagacg aagctaaaca gatatttaaa aaatatggtt
      961 ttgaacctgt taagtagtga ttaaacttga aaaataaatt ttttactact ctacttactg
     1021 gattttttgc tatagtcttt ttgtttattg tatcaccact tcttatgtta atcattaaag
     1081 ggataagtta tgttcctata tgcttaaaat cagtggaagt acagtatgca ataacactta
     1141 gtattaaaac atctttaata tctactatag tatgcctttt attagctatt ccagtagcat
     1201 attttttaca tattactaaa ttaccattta aaaaactgat tatacagata ataaatctcc
     1261 ctatgtctct tccacactta gtatcaggaa ttgctctttt acttttgttt ggaagaatgg
     1321 ggattggaga ttccatttat aagattttta agcttgattt tatatttaca aagcaaggta
     1381 tagttttagc acaaatattt gtaaatcttc cacttactat taaaatactt cacacttcat
     1441 taaatgaaag taatgaaaag atgatatttg ttgcacgtac ccttggatgt aatagttggg
     1501 aagcatttag atttataatt cttccaaatc taaaaactgg aatcatatct gcaactgtaa
     1561 tgacttggtc aagagcactt ggtgaatttg gtgcagtagc tatgatagca ggttctacac
     1621 gtatgaaaac tgaaattatt cctacatcta tatatttaaa tatgtctact ggtgatatag
     1681 acattgctat cggaatagct gtaattttga tatttctctc acttacatgc ttgatgcttt
     1741 ttgagatatt ttttaataga gaggttgata agaattaata tgttaaaaat aaaaaattta
     1801 tctcttgatt tacctaattt taaattacaa gaaaatagtt taaatattaa taatcatgaa
     1861 tactttgttt tattaggagc tagtgggtca ggtaaaactc tatttctaga aacattagca
     1921 ggaagatatc catctgctaa aggaaatgta tcatacaaag aaaatttaat atcaaactta
     1981 cctccagaaa atagaaatat aggatttgta tatcaaaatt ttgaattatt tccaaatatg
     2041 aatgttgaag ataatataag gtttccactt aaattgagaa aaatacctat agatgaacag
     2101 attcttaagg ctaaccactt gtctaaatta atgcagatag aagatataaa aaataggtat
     2161 cctaaaaatt taagtggagg cgaaaagcaa cgagttgctt ttgcacgtgc cttaatagct
     2221 gaacctgata tattactttt agatgaacct atgagttcat tggattatat tacaaaaaaa
     2281 catataagca aaattttgaa ggatgtctac ataaaataca aaccaacagt gattcatgtt
     2341 actcatgata tttctgaagc aatgtttttt gctcataaga taggaataat gaacaatgga
     2401 aaaataaatc atatttttga tttaaatgaa gaaataaaaa ataaaggaga atcttttttc
     2461 tatgaatata tttgatattt tgagagatta tctattagta caagtcgata aatataattt
     2521 aaatatggat atgataagta ttgtgtccaa atctctatct tcaaaagaag caattggaaa
     2581 tactaagcgt aaagatttcc caattatagt tggtaaagaa ataatgcttg aagctgactt
     2641 taaaggagct aaaggacaag catttacttc tactccatct acatttgaag gttctttaaa
     2701 agacatatta tcattagatt tacatgacaa ttctcatgat agaagtcttt ttatagcatc
     2761 attaaatgca gttatgaaat acttaggcaa gacggataga accattcatt gtaaaaataa
     2821 cgagccagag gtatgtgcaa aaaaattccc tgagtttata aaaatggaat ttggtaatcc
     2881 aaaagtagca attataggat atcaaccagc cataattgat aatattaagg actttttcga
     2941 aacaagagta ttagatttaa atcctgaatt tgtggatact attcaatata atgttaagat
     3001 tgaagatggt atacgagatt atgaagatgt aatatcttgg gctgatttag tgatttgcac
     3061 aggaagtaca ttatgtaata actcaattat aaattttcta tcacttaaca aaccagttta
     3121 ctattatgga actacgatag ctggtgcatc aaatatttta ggtttaaaaa gactctgttt
     3181 ttgtagtaaa taagcaatca tatcatcaag gagtacacaa atatatatgt gtactcttta
     3241 aatttaaaaa gttattactt ttattctaag tactaaagta aaattctatt aaaatgaatc
     3301 ccgaataaat gtacaatccc tttaaatctc aattcctatc aatcattaat attttgatta
     3361 gtcttataca taaatgtttt ctttatttta aactaataaa aaaattttat tccattaatc
     3421 tactctatat ttttcagcat ttaagaaata atttttagta ctttgtttga taatataact
     3481 aagacaacct ttcaggagat gattaaatga catttaagaa taaaaaattt actataaata
     3541 aatataaaat tctatctatt attctagttt tatgtttgat tttaaccgct tttagtagct
     3601 atgtgaatta tagtcataaa aaatttaaag attatgttgt cttctatgtt cagcatcaag
     3661 atgatgaagt gttgtgggct gggagtgcta ttgttaatgc tataaaagaa agaggaaaaa
     3721 atcatgtatt tgtggtttta gtatctactg gatgtggtat atccgtcttt gataaatata
     3781 aaaaatatga aaacctaaca aatatacaaa aatcagaata tagaaataga gaatttttag
     3841 cttctcttaa tagccttggt gtaataaggg aaaatataat attattacca gaaacaaata
     3901 caaatggtaa tactgacttc aattacatgg aaaaaattgc tcttacattt gaagaaaaat
     3961 ttaaaaatgt aactcatata gcacatactt ataaattaga tgaccattta caacatttaa
     4021 aaaatggttc tgttatccaa aatttatata atgctggtaa aataaaagat gctaaatatt
     4081 ttgtaaaacc taaatttgct gataaaataa cttttaataa taagatagtt tataaagctc
     4141 attctactga ggactatgaa aaggttaaaa atgcctgtca acaatacaaa attgttgatg
     4201 aattagaata tagagaaggt attggctata agtctgacca taaatcattt gataagttat
     4261 taaataataa agatgttcct gctatacttc atacacctaa tttataagaa atatttattc
     4321 tttaatttaa gttataaaaa ggaaattatt tgagattttg tgatatagca ctttttgtgc
     4381 tatactactt tttctcatac aactattacc catatctaat ttcatcatct cttaaaatat
     4441 ataaagatat catcattaaa acctataagc cttattaaat gctttataca tacctacatt
     4501 aaactacctg tttatattaa actacattaa aataaatatt tgttataaat tcttaataat
     4561 tatgaaactc atttgttatt tatatttgtt aatatatgtt taaacctaaa aaattaataa
     4621 accaaaataa cataagaagt gtgatagatt accctagata ttttcataaa cttttacctt
     4681 cagattaata tgggcgtcta tacttttaaa tttactatat gcattgacaa tatattaaaa
     4741 gtactagttt ttatttcata atgaacgggc ttactgtttt ctaatacctc tttttggtat
     4801 aaaataaaaa ttggttaaaa ataaaatttc aagggggtta ataaattgaa tcaaaatata
     4861 ttggttgtag atgatgatag agagatagtt gatgctatag agttctattt gagacctgat
     4921 aactttaatg ttttaaaggc atatgatgga cttgaagcat tggacatact aatcgaaaat
     4981 gatattaaat taataataat ggatgtaatg atgcccaatt tagatgggct taaaactaca
     5041 cttaaaataa gagaaaaaag gaatatacca ataattctct tatctgcaaa atcagaagat
     5101 atggacaaaa ttataggtct taatatgggt gctgatgact atataactaa accatttaat
     5161 ccactagaac ttactgctcg agtaaaatct caacttagaa ggtatataaa tttaggtgat
     5221 tttagtaata caaataactc aaatgatact aattatattc taaaaagtgg tggcctagag
     5281 ctaaatacag atacaaaaat agtttcttta gatggtgagg aaattaaaat gacaccactt
     5341 gaatataaaa tacttacact tcttatgtca cgaaaaggta aaattttctc ttctcatgag
     5401 atttatgaaa atgtctggaa tgaggatgct tatagttgtg agagaactat tgctgttcat
     5461 attagaagaa ttagagaaaa gattgaaatt aatccaaaag aaccaaaata tttaaaggtg
     5521 gtgtggggca ttggttacaa aattgaaaaa cttaattatt aatgattcta aagcaaaatt
     5581 tattttatta ttattactgt ttctattagt ttctatatta tttgaagcag tttatgattt
     5641 gaataataac ttttctaata ttaattatta ttatagagag tttgctctcc cttattttta
     5701 ttttttcggt actcctgtac atttgtcaaa agtagaatta gagtttgtct gcataggtat
     5761 tatattatca agttttttat tgactatact atgttttaca ttgtataaaa aaaataaaca
     5821 aactaaagtc aaattcttag agaatgtcat aaaccttttt tctaaggtaa aaatagaact
     5881 tacacttata ttaactttga tactattaac cttaaggttt catgcattta caagtaataa
     5941 aatgttttac aacactaatt taataatcta caattttata atta
//
LOCUS       DAMJVI010000160         5863 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.165, whole genome shotgun sequence.
ACCESSION   DAMJVI010000160 DAMJVI010000000
VERSION     DAMJVI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5863
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.165"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            129..1493
                     /locus_tag="PC545_003464"
     CDS             129..1493
                     /locus_tag="PC545_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDF2458181.1"
                     /translation="MDIKELVKMQRKYYNTGKTRDISFRIEQLKKLKLVVSQNEEKIL
                     LALKKDLNKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLVAGNCVILKPSEYSVSTSSVVKDIVEDTFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIIMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDRKNELIEKIKYYTNKYFGDNPC
                     NNEQFPKIINQRHFNRILSLIDKDKIVYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKLLEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKVL
                     KKIM"
     gene            1632..2798
                     /locus_tag="PC545_003465"
     CDS             1632..2798
                     /locus_tag="PC545_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDF2458182.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAIF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGLLLTYATATLIL
                     YLFMHFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIIGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVMFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVESKLDKKGLPTDTSDYM"
     gene            2915..3697
                     /locus_tag="PC545_003466"
     CDS             2915..3697
                     /locus_tag="PC545_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDF2458183.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQSSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMAYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYADSLGVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3816..4469)
                     /locus_tag="PC545_003467"
     CDS             complement(3816..4469)
                     /locus_tag="PC545_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2458184.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4493..5857)
                     /locus_tag="PC545_003468"
     CDS             complement(4493..5857)
                     /locus_tag="PC545_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HDF2458185.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSVFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLAAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKGATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
BASE COUNT         2199 a          745 c          900 g         2019 t
ORIGIN      
        1 tttttttatt ggaagattta tgttatattg agatgaattt attatgatat aaaaaataat
       61 gttaggataa atttaaatct ttttctaaaa atgtattaat ataattctta aagaaaggat
      121 ttttaataat ggatataaaa gagttagtta aaatgcaaag aaaatattat aatacaggta
      181 aaacaagaga tatatcgttt cgtatagaac agttgaagaa acttaagtta gttgtatcac
      241 aaaatgaaga aaaaatactt ttagcactaa agaaagattt aaataaatcg gattttgaag
      301 gttttatgac agaagtaggc atgttttata gtgaattaaa ctttgctata aagaatataa
      361 gaaaatggag caaaataaaa agagttaaat caagtatggt taattttcca tctatatcca
      421 aagttgttcc acaaccttat ggtgttactc tcataatgtc accttggaat tatccatttc
      481 aattagcact tataccatta gtttggtcat tagtagcagg gaattgtgtt atattaaaac
      541 cttctgaata ttctgtgtca acatctagtg tagttaaaga catagtggaa gatacttttt
      601 caaaagaata tgttgcagtt gttcaaggaa gtcaagaaga aagtgaaaag ttactattgg
      661 aaagatttga ttatatattt tttacaggaa gtactaatgt tggtaaaata atcatgaaat
      721 cagcatcaga gcatttaact cctattactt tagaacttgg aggaaaaagt ccttgtatca
      781 ttttaaaaga tgctaatata gaccttactg ccaaaagact tacttgggga aaactaataa
      841 atgcaggaca aacctgcgtg gctccagatt atgttttagt gcatgaagat agaaaaaatg
      901 agttaataga gaaaataaaa tattatacta ataagtattt tggagataat ccatgtaata
      961 atgagcaatt tcctaaaatc ataaatcaga gacattttaa tcggatatta tctcttattg
     1021 ataaagataa gatagtctat ggtggaaatt ataataagga gacattaaag atagagccaa
     1081 ctatagtaga caatgttaat tgggatgata atataatgaa ggaagaaata tttgggccta
     1141 tatttcccat tttaacatat aaagatttag atgaagtaat acaaaaaatt atacaaatgc
     1201 caaatccatt ggcactttat atttttacaa aaaataagta cctagaaaat aaacttttag
     1261 aaatgattcc agcaggcgga tgttgtatca atgatacagt tactcatata gctacgaatt
     1321 acttaccttt tggtggtatt ggtgaaagtg gtatgggctc atatcatgga aaagctggat
     1381 tcgatacatt tactcactat aagagtgtgt taaaaaaatt aaatttggat gtccctatta
     1441 gatatgctcc atacgataat aaattaatta aggttcttaa aaagataatg taggttatat
     1501 tgtagaataa taaatataaa taaaattgag tatttgtatt ctgaatatat tttattttta
     1561 atttggtata tttgttataa aagtggtgat tttgtgataa aatttaatta tatagaaata
     1621 aggaggaaaa aatgaaaatt aattggaata caaaatacac aacaatagca atttatacat
     1681 ttataatagc tgctagtagt attatatttt atctagtatc atcacaaatt gatgtatttt
     1741 ctaataattt agatgctata tttacaacat tacaaccttt tataatagga tttgctattg
     1801 catatcttct taattttatt ttaaagtttt atgaagacag aatttttata aaaagtgaga
     1861 aattaaaaaa actaaaacaa agttcaaaaa gaggcttggg tcttttatta acatatgcaa
     1921 cagctacatt gatactttac ttatttatgc atttcgtatt gccacaagta attgagagta
     1981 ttgttggact agcaaatgat atacctatgt atgtaaacaa tgcaactaag cttattgata
     2041 aattaatgac tgatttaaat cttgatgagc aatactttaa tttggcagtt gataagtgga
     2101 atgagtttgt tacatatatt ataaagtttg taacagattt aatcccaata ttaggaagta
     2161 tgttaaagaa tgtagcctct agtatatgga atattgtact aggattaatt gtttcagtat
     2221 acttattaat tgataaagaa aaattctatg gattaagcaa aaaaataact tatgcagtat
     2281 ttacagaaaa acaagctgcc agaattttag aattaactca tagaagtaac tatacatttg
     2341 gaagattttt aggtggcaaa atattagatt cattcattat aggtattcta acatttgtaa
     2401 tacttacgtt agttaaaatg ccatatacat tattaatatc agttataatt ggtattacaa
     2461 atattatccc tttctttgga cctttatttg gagctatacc atctacgcta atagtgatgt
     2521 ttgtatctcc tataaaagct ttctggttat tgttgattat attaataata caacagatag
     2581 atggaaatat tataggacct aaaatacttg gagattcaat tggtatatct gcattttgga
     2641 ttttgttctc cttattggtt gcaggaaaat tattaggttt tattggaatg gttataggag
     2701 ttccaatgtt tgcagtaatt tattctataa ttaaggatat tgttgaaagt aaattggata
     2761 aaaagggatt accgacagat acttccgatt atatgtagct aaaaaatatt atgtataatt
     2821 aaaaatatat aaattaagca atatttaaag cactgagaaa gttaaactct tggtgctttt
     2881 tagatataaa attattatag aagaggtgtt ttttgtggca tttatagaat gtaaatttaa
     2941 atctaaatat actggaggag aaactgatat aattgtaatt ttgcctgtag caaatggaaa
     3001 agaacttttt agtggtaaag agatatatga ttttgaaggt aaatttaaaa cattatactt
     3061 atttcatggt ttagaagatg accaaagctc atggattaga tatactaatg tagagcatta
     3121 tgctaaaaat aaaaatatag ccctagtaat accaagagta gaaactagtt tttataccga
     3181 tatggcgtat ggacaccaat attttacttt tgtaagcgag gaattaccta aatttgttag
     3241 agctatattt cctttatcag ataaaaggga agataacttt gtagctggta tgtctatggg
     3301 gggatatgga gctttaaaat tagcgctttc aaaaccaaaa gagttttcgg cagttgcaag
     3361 tttttctggt tcgcctgata tgattcatga gttagaaaat caacaaatag aaaccaatgc
     3421 agacattcta tttcacgatt ttggtagacc agaagacgct agaaatagtg agaatgattt
     3481 actttacata cttaagaacc ttaaagaaag aaatgaagat attcctaaaa tttatcaatt
     3541 ttgtggtgat agagattttt tatataaaaa taatcagatg tttaaaaaat atgcagattc
     3601 tctgggtgta gatgttgaat atgaagagtg tgtagggggg catgaatggc gtctttggaa
     3661 tttatggata aagaaattta tagatataat tggatagtac acttatggta ttaacaggta
     3721 tactataaaa tatctttaaa tatgataaag actgaaatcc taaaatcaaa ttatgatttt
     3781 aaaatttcag tctctaagaa tttaataaaa ttttattaag ttcttacacg agttattatt
     3841 ggagtaaaat tgtcaactat atctatatag tatttactca attcctctaa aatctcttcg
     3901 cataaatccg tactatgacc aagattaata agaaactgtt ttgtaaaatg ctttattaca
     3961 gcttgagaag atatatcgta cttgtgtgaa gtataaactc taattatttc aggtttcata
     4021 gcaagtatac caatacaaga aagtattaat tcatcatgtt ctatagattc tttatcatta
     4081 acatatcttt tcatgaccat ttcggttaca cttctttgat agcttaaaat agcttcttca
     4141 taagctatat ccaaataaaa atttctatat gcttcgtttt ttagaaggaa acaaaggtca
     4201 gcattcatag caagaaagaa catcatcata gtaccttctt catggtagtt atagattaaa
     4261 gactgcattt catttcttat atcttcatat attatagctc ctatttcgct tttacttgag
     4321 aagtaatagc ttattagacc taaatttata ttagctcttt ctgcaatttg cctagttgtt
     4381 gtcttatgat aaccttgctc aaaaaataat tctctagcag cacttataat actagctttt
     4441 gtttctttgc ccttttctga ttttgacata tttactgtgt atacctctct tttcaatctt
     4501 tttttgaaaa tttgctaact ataaatattg taatagaacc acctattata ggaaccaaaa
     4561 ttctgtcaaa tacagtaaga ataccaatca tatttaataa tgccaatata acacttacta
     4621 taacagcata gatagctcct ttttttgtag cacctttcca gcaaagacca attacaaatg
     4681 gtgcaaatat tacacctgta cataatggag ctggccaaaa caataattct aataaactat
     4741 caaatttaag tgctatgatt gcacctatta tagctgtcaa agctgatgca atttgagaat
     4801 actttagagt attttttaaa tcattaaatt ctatattagg atgtaataat ttgttataaa
     4861 tatctcttga tataatagca ctcattgagg tcattaatga atcggcagta gacattatgg
     4921 cagccaatat agcagctaag aataatacag atattaaagg tggcaaagta gtcgatatca
     4981 tttgaggtaa aacctgaaca gatgatatat ttggaaataa actaccacca atagctccaa
     5041 ttattcctat agggaaagca agaaataata gtataaatcc tgcaattaca ctacttttaa
     5101 ctgcattttt agcattatca gcacattgat atcttaaaaa actttcataa gaaaatattg
     5161 agcctaaaac taatggtaaa actgtataca taaaatcact aatcgattta tttcctggaa
     5221 agaatggttc ttttaccatt gattgtaaca taagtaaagg gttgtcgcca actaatttta
     5281 agcttatata agctactatt ggcaaaaaca ctatacaaat agcaagttga ataaatgctg
     5341 atactgcaac tccccacatt cctgatgtat aagtatatat aatgataact ataactccta
     5401 taattacagc tatatcataa ggaataccaa atgcagtaga tacagaacca aaagaaatca
     5461 attgactacc cataatagct agtatagata ttggtgttac aagactttga taagctctta
     5521 aaaatacact atgaccatat ctagtttcaa tataatcagc aggtgttata tcacaagata
     5581 agcttcttaa ttttcctgcg aaaaatgcat ttataaagca agctaaagct gcacctatag
     5641 tagaatacat tccaacccaa agtcctcttt cagctcccca ttgaactcca ccaactaagc
     5701 accctcctcc aaaagatgta gcagcaatgg ttgcagctag tataggcaaa ggcagatttc
     5761 tacctgcgat taggaaatcg gatgctgttt taactttttt tgaaaaataa aaaccaatcc
     5821 atatgtatat tgcacacgta acaattaaaa gtaacatagt ctt
//
LOCUS       DAMJVI010000161         5814 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.166, whole genome shotgun sequence.
ACCESSION   DAMJVI010000161 DAMJVI010000000
VERSION     DAMJVI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.166"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(297..2129)
                     /locus_tag="PC545_003469"
     CDS             complement(297..2129)
                     /locus_tag="PC545_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458186.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILTFLLFLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISAKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAVLILPISMFVISRIIKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEVQIVENAVSIDGLEGKIDFENVNFGYNPNKTIINDFSVNVE
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYKMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(2122..4368)
                     /locus_tag="PC545_003470"
     CDS             complement(2122..4368)
                     /locus_tag="PC545_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458187.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELNKITLFMDKSSKDKVLDNYTLLNKKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNVIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFESMPESMITQSSVSYVRSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMISMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEAQEFKEDKKGLVEFKNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKDATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(4436..4897)
                     /locus_tag="PC545_003471"
     CDS             complement(4436..4897)
                     /locus_tag="PC545_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2458188.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNKVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEDEKYF"
     gene            complement(5202..5768)
                     /locus_tag="PC545_003472"
     CDS             complement(5202..5768)
                     /locus_tag="PC545_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HDF2458189.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         1857 a         1082 c          632 g         2243 t
ORIGIN      
        1 attaaaatta aattctattt ataataaaat ttaaagaata aaattaaata tacattttga
       61 taatcacctc caacattttc cttactacat attataataa aataaacact ttgtaaagac
      121 ataaaattat gtggctatct caaaatatag agaatcctct ttttcattgt taaaatgata
      181 aaaatacact ttatttcata cacaaaagag tgccttatga attaaaaagt tcattttaag
      241 gcaccctcat ttattttatc ttattaatta catgtctatg ctaagatagt tttttgttac
      301 attgcttcat cttcttcaaa ttgagaattg taaagattgg catagaatcc gcctttcttt
      361 agaagttcct catgatttcc ttgttcaact atatctcctt cattcattac aagaatcata
      421 tcagcatcac gtatagttga taatctgtgg gctataacaa aacttgttct accttccata
      481 agattatcca tagctttttg aatcaatact tctgttctag tatcgacaga acttgtagct
      541 tcatctaata ttaaaatttt aggatcagct aaaatagcac gagctatagt caataattgt
      601 ttttgacctt gagaaacatt acttgcttct tcatttaatt tcatcttata tccatctggt
      661 aatgtcttaa taaatctatg aacatgtgca gatttagcag cctctataac ttcttcatct
      721 gtagcattca acttaccata tcttatattt tccataattg aaccactaaa taaccatgta
      781 tcttgaagaa ccataccaaa catttctcta agctcactac gattaaaatc tttaatatta
      841 tgaccatcaa tcagtattga accgctatta acatcataaa atctcattaa cagtttaact
      901 attgtagttt tacctgctcc tgtaggtcca acaatagcaa ctttttgacc aggttcaaca
      961 tttactgaga aatcatttat tatagtcttg tttgggttat atccaaagtt gacattttca
     1021 aaatcaattt taccttctaa accatctatt gaaactgcat tttcaacaat ttggacttct
     1081 tcttcctctt ctaaaaactc aaatactctt tcagaagctg ctgctgttga ttgcaataaa
     1141 ttggcaacct gtgcaatctg agtcattggc tgtgtaaagt ttcttacata ttgtataaat
     1201 gactgtatgt caccaacctc aattgttttc ttaattgcaa gccaacctcc taagattgat
     1261 accattacat atcccaagtt tccaataaac atcataagtg gttgcattat acctgataaa
     1321 aattgagatt tccacgctga attatacagg ctatcattta gtttatcaaa ctcttcaatt
     1381 acttcatcct cacgattaaa tgcttttact attgtttggc cactgtaagt ttcctcaact
     1441 tgaccattta catttcctaa atattcttgt tgatatctaa agtatttttg tgatttcttt
     1501 attattcttg aaattacaaa cattgatatt ggtaatatta aaactgccac taatgtcata
     1561 attccactta tagataacat cattatcaaa acacctatca tagttgttac tgatgttata
     1621 agctgtgtca tactttgatt taaactttga tttaaagtat ctatatcatt cgtaattctc
     1681 gataatattt caccatgcgt cttagtatca aaatacttca ttggcaatct atccaatttt
     1741 gctgaaatct cttttcttaa attataggat actttttgag aaattccact cattataaat
     1801 ccttgtataa aagagaacag tgcacttatc aaataaagga aaagtaaaaa ggttaagatt
     1861 tttccaattg cattaaaatc tatacctacg tttcctccag aaactttact cactagccct
     1921 tcaaatatct ttgtagttgc ctttcctaaa atttttggac ctactataga aaacgacgca
     1981 ctaccaatag caaatacaat tactagaatt attgatattt taaatttaga taaatatgca
     2041 attagttttc gcatagtccc tttaaagtca tttgcttttt gtcctgctcc catagatttt
     2101 cccataggac ctcttcccat tctactcatt agctagctcc tcctttgaaa gttgtgataa
     2161 agctatttgc tcataaactt cgcaattttt tagaagctct ttatgtgttc ctattccaac
     2221 tatttcgccc tcatttaaaa caattatctg ttctgcattt aagatagtac taattctttg
     2281 tgcaactatt agaactgtac tttcagcagt ttttgatttt aaagccttac gaagagctgc
     2341 atcagttttt aagtcaagtg ctgaaaaact atcatcaaat atataaactt ctggattttt
     2401 tgctattgct cttgcaatag aaagtctttg tttttgacct ccagatacat ttgttccacc
     2461 ttgagaaatt tcagttttaa aagcttccgg tttagaatct ataaattctg tagcttgagc
     2521 tatttcagct gctgttctca tttcttcatc tgttgcgtct tttctaccat attttaagtt
     2581 ggattctata gtaccagaaa acaatactcc cttttgaggt acatatccaa ttttttccct
     2641 taaatcatgt tgagaaacat tttttatatt aactccacca agtaatattt ctccttcagt
     2701 aacatcaaag aaacgaggaa ttaaatttat aagagttgac ttaccactac cagtacttcc
     2761 tataaaggca gttgtttctc ctggctttgc tgtaaaagat atatttgtta atataggttc
     2821 ttctgcgtct ggatacatga acgaaacatt tttaaattca acaagaccct ttttatcttc
     2881 cttaaactct tgtgcctctg atttttctgg gtcttttatg cttatatctg acactaatac
     2941 ttcatctata cgtgtagctg aaacggatgc tcttggaagc ataatagata ccattgatat
     3001 cattaagaat gacattatta tttgcattgt atattgtata aatgccatca tatctccaac
     3061 ttgcattgct ccagaatcaa cactgtatgc acctctccaa actataagaa ctgtgataag
     3121 gttcattata agcatcatag caggcatcat acatgtcata actctattta caaaaagatt
     3181 tgttttagtt aaatttttat ttgctttatc aaatctttct tcttcatgtt tttcagtact
     3241 aaaagctctg ataactggaa taccagtaat tatttcacgg cttactaagt ttaacttatc
     3301 aacaagcttt tgaaccagtt taaacttagg cataaccact gagaataaca ctacaattaa
     3361 tgataagata gcaactactg ctactgctat aatccaggcc attgaagtat ttgtgtttaa
     3421 aacctttatc actccaccta tagccattat aggtgcatag aatactattc taagcaacat
     3481 aaccatcaac atttgtatct gttgaatatc atttgtacta cgagtaatta atgaagctgt
     3541 agaaaattca ttcatttcag tatttgaaaa tgacataact tttctaaata ctccacttct
     3601 taaatttcta cctacacttg cggctactct tgctgctaaa aatcctactg ttattgtagc
     3661 taccatacta atcattgcta taccaagcat tttagcacca gtaaataata tataattatt
     3721 ttgagttttt tctgtatcaa tacctatttt ctcatattct gaccttacat atgaaacaga
     3781 gctttgtgtt atcatactct ctggcatact ttcaaagcct ttgctcatct ctttagtcaa
     3841 tgtatctaac tgttcctttg gcattgctga taacaattta aatatatcaa catctccact
     3901 ttgagtagct tgtggtggaa gtttactcat taactgagct ttcatagctt ttacctcttt
     3961 actatcgccc tcaagccctg atactattaa aatagcttta ccaaaaataa catttaaatc
     4021 atctatagtg tctttgtctt tagtatttaa ctcataaaga gattcatctt ttagacctgg
     4081 gtacttatct ttatacttaa cataatcctt tttattcagc aaagtataat tatctagtac
     4141 tttatcctta ctacttttat ccataaataa agtaatttta tttaattcac tctctctaat
     4201 cacagatgga acagagtttt ctactccacc ttgttgtata cctacattta ctatattgga
     4261 tgtgtattct ggtaaagaaa ggtcacatac cgcttgaatt accaatagac agactattat
     4321 tactattgat aatgccgatt tctttaaata tttaattaat ttaaacatac atttcttccc
     4381 ttctgtaata ttaattagtc taataacatt aaattgaaaa acattatttt aaactttaaa
     4441 aatatttttc atcttcatgc ttatgacatc ctgcttttaa attatctcgt acttgcatta
     4501 gaagtcttct aagtactatt ttttcttctt cactaaatcc taaaaaaaac tctctttcca
     4561 attccttgtt aaattcttca actttattac atatttctaa acctttgtct gtcaaaaaaa
     4621 tcctagatac tctttggtcc ttttcatctt gttttctttc cacaagtcca gctttttcca
     4681 tcctctttat cataactgta gttgtagatg cttttacttt ccttttatct gctaattctt
     4741 tttgactttg cccatctttc atctttaaag aaaaaagtat ttggggttga ccatgatata
     4801 agcctaattt atcaaaataa aaatgagttc ttataaaata tattcgcatt aattgcaaga
     4861 aaacatcatt taatttttct tcagaattgc tattcatact tgccctcctt attttttata
     4921 ttacttagtc ggctaagcat tattttacta tactgcatgc attttttcaa tatatttata
     4981 aaaaattcat ataaatttca tttattaaat atttttatat catatagtta taattttaat
     5041 aaataatagt aagattttta attagtcagc tacttatatt ttttattgta aagcttaaaa
     5101 taaacctttt catatatttt caaataaaaa agttgtccca aaataaattt tgaaacaaca
     5161 tcttattcaa cagtatcttg attatataac attatgatac tctacttatc ttcatatcca
     5221 tttacaacaa catctaactt cccagtttca ggtgatataa ttagaccatg tacaataata
     5281 ccatctggaa ttaaaggatg cccttttatt aaatcaatac tatcttttac agattcttct
     5341 gcagaatcga atccatgtaa ccattgtttt gtatcaattc cagcactacg aagtgtaagt
     5401 attacatcat tagatattcc tctatctaaa attttaccaa gtaacttatc cgtatcaaca
     5461 ttacacatac cacatccatg atgccctaca accataacct catctacatc aaattcgtaa
     5521 attgcaacta aaacacttct cataatactt ccaaaaggat gcattatagt agctcctgca
     5581 ttttttatta gtttcacatc tccatttttc aaattcatag ccttaggcaa aagctcagtt
     5641 aatcttgtat ccatacaaga taagactact atttttttat ttggatgttt tgatgtcaca
     5701 tattgttcat attctttgtt ctctacaaat gatttattat attctaatat ctcttcaagt
     5761 tttctcatat gtaatatcac cctctttaaa aattttttta ttattatatc acta
//
LOCUS       DAMJVI010000162         5724 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.167, whole genome shotgun sequence.
ACCESSION   DAMJVI010000162 DAMJVI010000000
VERSION     DAMJVI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.167"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            235..417
                     /locus_tag="PC545_003473"
     CDS             235..417
                     /locus_tag="PC545_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458190.1"
                     /translation="MFKDKIDECVHIMTAYIASLKEYYSFIETQIGDFIKKYGEDVVE
                     LCLHRVMILLCECGLA"
     gene            1249..2163
                     /locus_tag="PC545_003474"
     CDS             1249..2163
                     /locus_tag="PC545_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HDF2458191.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTNKNYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSVAEQKTEEFTGIEINKNITT
                     RELNLQSGVNPQVGQDLVNYVAQLNSDAIRLLDGLINFKERVLDGVLSCTIFTSNYPL
                     LLEHIIHEANLYRYYVVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFAIKFNELIEKTNEMTDSNIKNITEETLNETVEFKDFKEAGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            2553..3200
                     /locus_tag="PC545_003475"
     CDS             2553..3200
                     /locus_tag="PC545_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HDF2458192.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEMGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESICAYMVTTRISRPAKKIKVILVGENLGL"
     gene            complement(3367..3936)
                     /locus_tag="PC545_003476"
     CDS             complement(3367..3936)
                     /locus_tag="PC545_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDF2458193.1"
                     /translation="MKKFIICIIMFAISVLVLAGCTNDNKIKNEVDSMFNSIKTQDAD
                     TVDKYFPKSGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKINNTDMVSIL
                     KKNPPKLGEMMPNPTMKDIDSAKKKEFNITLELVKSGDNYVCKVNQESAMKLLNVITG
                     GGIDYLADYNKDYYKQLDEEQKNYENKNS"
     gene            complement(3941..4840)
                     /locus_tag="PC545_003477"
     CDS             complement(3941..4840)
                     /locus_tag="PC545_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDF2458194.1"
                     /translation="MKLLSKVNNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKKLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLHKVPFPVKIKKVYGDKYLKYLKYIVLIMFVIILPVAIVNVAGGGNPWFCKW
                     ICPSGTLLGGILLILGNENLRESIGFLFSWKLSILLLILLMSIITYRPFCKYICPLGA
                     IYGFFNPISIYRFKIDKDKCTNCTACQTKCKLDIPIYKDVNSPDCIRCGECIKVCPQN
                     AITTTFSKDKKENNICAKKYRGI"
     gene            complement(4851..5000)
                     /locus_tag="PC545_003478"
     CDS             complement(4851..5000)
                     /locus_tag="PC545_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458195.1"
                     /translation="MSNFIKDNKKGISIIAISIIFIALGTYRGETNTVLTKAIMICLE
                     CIGIG"
     gene            complement(4993..5577)
                     /locus_tag="PC545_003479"
     CDS             complement(4993..5577)
                     /locus_tag="PC545_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDF2458196.1"
                     /translation="MKRFFIVVLGCICVLGMISGCSKKESKPVEKNKNNSEWFSNLDT
                     EDVDGNKVTKEIFSNKELTLINVWTTWCGPCVGEMPELEALSKEYESNNSNVAIKGLV
                     VEVDKTDMRTGLSNEEKNLVKDIMKKSDATYQQLTVSEELKKTDFKRVMEFPTTYFVD
                     KKGNFVGEKVVGANSKEDWKKIIDERLKMVKANE"
BASE COUNT         2098 a          863 c          661 g         2102 t
ORIGIN      
        1 tattaattta tattaaatat ctactaactc aaatattcat aattaaactt taaactggat
       61 aaattttctt ttaagaatga aagtttatcc agttttgcga ttataataac cagttttaca
      121 tatccacttg taaaatatat ctaagcaata tatcatacta tatagattta tgagtgaaat
      181 aatatctgct atttactgaa tatttttatt tttcgttttt tagggggatt agttgtgttt
      241 aaagataaga ttgatgaatg tgttcatata atgactgcat atattgctag tttaaaagaa
      301 tactattcat tcatagaaac tcaaattggt gattttatta aaaaatatgg agaagatgtt
      361 gtagagttat gtctccatag agtgatgatt ctactatgcg aatgtggttt ggcataaaat
      421 taaatttatt aaaatgggta agaagaatcc tatataatat tcttcttacc cattttaata
      481 tatcaatttc atagcttatc gtttaaaata ttttacatcc attactagac aactttacat
      541 tctcttttta attctagtat atttaaatca ttttagttgc tccttattta ttcttattac
      601 ttgttttatt ttataaataa aataattctt tttaataatg ttttttgttg atattcctat
      661 cacacatcca attatttatc attgtcttga aattgtttgc atacttaatt taatccaatt
      721 gaaacacccc agcatagaat cccctgtaaa atagaagttg gattaaatcc taacatctat
      781 acagaaaata ctactccaag cacaagcaat ataatggaat atatttatca cttaattttt
      841 tatacttttt aaatcccata cctaaagcat taagtgcagc tattagaatt aataattgtt
      901 gtggtataaa attatgcttg cttcatattt attcattttt tactataaaa gttcttgttt
      961 tacaaaaaac atttgttttg tatcaaaata atacttgtaa attacataat tctcataaaa
     1021 atttctagta ttacttgtac tcttaataaa taatagccaa caagtacctt aaatcattat
     1081 atctatatct aataagaaac acagtaagat aattttttcg cccataactt aataaaaact
     1141 gttgatatta gtagcaattg aatttagtta atttatttat aatatacata aatttaaata
     1201 ttacgaataa agtattataa ataggatact ttctaaaggg aggaaattat gatagataat
     1261 caaaaatatg ttattttatc actagaatta catttatttt tttcaagaat tatgaaagag
     1321 catgctcttt ttttagaagc aggattcaca aataaaaatt ataatcttgc tatggaagct
     1381 gaccactata aaaagcaatt tgaagattta ttatcataca ctgttagtgc tagtaatggt
     1441 ataattagac ctgatatatt atattcagaa gagcttgtaa ctactctcac atcagttgca
     1501 gaacaaaaaa cagaagagtt tacagggata gaaataaaca aaaacatcac tacaagagaa
     1561 ttaaatctac aaagtggtgt aaacccacaa gttggtcaag acttagtgaa ctatgtagct
     1621 caacttaact ctgatgcaat aagattactt gatgggctta ttaattttaa agaaagagtc
     1681 ttagatggtg tactatcatg tactatattt acatcaaact accctctact tcttgaacat
     1741 ataatacatg aagcaaattt atatcgttat tatgtagttg accttgaaaa taaaatagat
     1801 attgagtcaa aaaacgctaa agaaatagaa ttattctggg accatattat gatggaacat
     1861 gctctgttta tgagaggatt actagacccc tcagaaggtg aactaataaa tacttcaaat
     1921 gattttgcta taaaatttaa tgaattaatt gaaaaaacaa acgaaatgac tgattctaat
     1981 atcaagaaca ttacagaaga aactctaaat gaaactgttg agtttaaaga ttttaaagaa
     2041 gcaggagcat caggaataga acagtgtaag ataaaatcta taatattacc acttttagca
     2101 gaccatgttt taagagaggc aaatcattat attagaatat tggagagtta taaaaacatg
     2161 taattaaaaa ttacttttaa aataatcttt gcatatttag ataaaataaa ggtacagtta
     2221 actgtacctt tattttttat aaacttataa aatttaaaca agttattcat aaaataaaat
     2281 aataatcatc tataacccac aactttattt tatattaaat aaaaagtatt agtgattatg
     2341 ttttataggt atttctttat tcagttattt attaaaaact aatagtaaat cttatactaa
     2401 tcgcatattt atgttataac tatattgtaa ctattttcgt aatcttagga aaagattact
     2461 tataaaatta accttataca ataatatatc gtataatctt aattagcata atagttacaa
     2521 tattaaaatt taaatatggg agtgatttta gtatgataag acaaacacca ttagagaaac
     2581 gatatgacaa gctaggtcct catattgtaa cagcactcaa aaaacgttat tttgatgctt
     2641 attactgtaa aactagaaat gaagctttgc aacaaatttt agatttaatt cctcaaaatc
     2701 atgtagtatc atggggagga tctgaaacac ttaaagaaat gggcgtacaa actgctgtta
     2761 gagagaaagg atttaaagtt attgatagag atttagcaaa aactccagaa gaaagaactg
     2821 aaattatgcg tcaagcactt ctatgcgata cttttttgat gagtagcaat gcaattagtg
     2881 aagatggaca actctttaat attgatggaa atggcaatcg tgtagctgca atgatatttg
     2941 gtccaaaaaa tgttatagtt gtagctggta tgaataaaat tgtaaaaaac attgatgatg
     3001 caatacaaag aactagaaca attgcatctc cagcagttgt tcagcgtttt caagatgtca
     3061 atacaccatg ctatattaat ggaagttgta tagattgcac aagccctgaa agcatttgtg
     3121 catacatggt tactacaaga atcagtcgtc cagcaaaaaa aataaaggta atattggtag
     3181 gagaaaatct tggtctataa aatatacata catgtaaata gctctataaa taataaaatc
     3241 atatgaaata acttatttct aaaagtggta gaataaaagc ttctacaaga ctttcattct
     3301 accactttta tattctttta atttgtgttt ctatattatt aattgctccg attttcatat
     3361 atattactaa ctatttttat tttcataatt tttttgttct tcatcaagct gtttataata
     3421 atctttatta tagtcagcta gataatctat accacctcct gtaatcacat ttaataattt
     3481 cattgcagat tcttgattaa ctttacaaac ataattatca ccagatttaa caagctctaa
     3541 tgtaatatta aattcttttt tctttgcaga atcaatatct ttcatagttg gatttggcat
     3601 catttctcct agtttaggag gattcttttt taaaatagaa accatatcag tattgttaat
     3661 ttttaaatct acagtcactt tatccttatt attctttata tctgaaattt cccaagtcat
     3721 atttttagaa tacattttaa atccttcttt atcttcgtat ataagattac ctaaccctga
     3781 ttttgggaaa tacttatcaa cagtatctgc atcttgtgtt tttattgaat taaacatact
     3841 atccacttca ttttttatct tattatcatt agtacaacca gctaacacca gtactgatat
     3901 tgcaaacatt ataatacata taataaattt cttcattaaa ttatatcccc ctatactttt
     3961 ttgcacaaat attattctct tttttatctt ttgaaaaagt tgtagtaatt gcattttgtg
     4021 gacaaacttt tatacattca ccacacctaa tacagtctgg actatttaca tctttatata
     4081 ttggtatatc aagcttacat tttgtttgac atgctgtaca atttgtacat ttatctttat
     4141 caattttaaa tctatatatt gaaataggat taaaaaaacc atagattgct cctaaagggc
     4201 atatatactt gcaaaatggc ctataagtaa tgattgacat caataatatc aatagcaaaa
     4261 tacttaactt ccagctaaat aaaaatccta ttgattctct taaattctca tttcctaaaa
     4321 taagtagtat tcctcccaaa agtgttcctg aagggcaaat ccatttacaa aaccaaggat
     4381 ttccaccccc tgctacattt acaatagcaa ctggcaaaat aattacaaac attattaaga
     4441 ctatatactt tagatatttt agatatttat caccataaac tttttttatc tttactggaa
     4501 agggtacttt atgtagtaaa tcttgtacaa gtccaaaggg acaaagccaa ccacaaacaa
     4561 atctgccaaa aaaagcacca aatgccatta aaaaaccaac aacataaaat gagaatttat
     4621 actctctgct tgataaaact gcttgtaatg aacctattgg acatgctcca agtgcacctg
     4681 gacatgaata acaattcaat ccaggtacac ataatttttt actttctcct ccatatatag
     4741 ttccttctaa aaatccattt acatatccat ttgttaatgc tgtaaatgca acttgagtaa
     4801 taagtcttat attattattt accttagata ataacttcat ccctcttctc ctaaccaata
     4861 ccaatacact caagacaaat cataattgct ttagttaata ctgtatttgt ttctccacga
     4921 tatgtaccta atgcaataaa tataatacta attgcaataa tagaaatgcc ttttttatta
     4981 tctttaataa agttactcat tggcttttac catttttagt ctttcatcta taattttttt
     5041 ccaatcttcc ttactatttg cacctacaac cttctcaccc acaaagtttc ctttcttgtc
     5101 tacaaaataa gttgttggaa actccataac tcttttaaaa tcagtttttt taagttcttc
     5161 agatacagtc aattgttggt aagttgcatc tgactttttc ataatgtctt taactaaatt
     5221 tttctcttca tttgaaagac cagttctcat atcagtttta tcaacttcta caactaatcc
     5281 ctttatagct acattggaat tattactctc atattctttt gataatgctt caagctctgg
     5341 catttcaccc acacatggtc cacaccaagt agtccagaca ttaataagag taagttcttt
     5401 atttgaaaat atctcttttg ttaccttatt tccatcaaca tcctccgtat ctaaattact
     5461 aaaccattca ctattattct tgtttttttc tactggctta gattcttttt tgctacatcc
     5521 agaaatcata cctaaaacac aaatacatcc taaaactact atgaaaaacc ttttcatatc
     5581 tcacactcct agaatttata tagtatataa aatcgctgct tgaaataaaa ataacttatc
     5641 ttctatatta aagtaattat atttaataaa taagagctaa tttaaaaaaa gatatacagt
     5701 taaaatctat ttcttttcag acaa
//
LOCUS       DAMJVI010000163         5722 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.168, whole genome shotgun sequence.
ACCESSION   DAMJVI010000163 DAMJVI010000000
VERSION     DAMJVI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.168"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            166..666
                     /locus_tag="PC545_003480"
     CDS             166..666
                     /locus_tag="PC545_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDF2458197.1"
                     /translation="MRKNSINIALTAAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLV
                     IASLWPTVINSIVVGLMLNVFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     VINIKF"
     gene            723..1709
                     /locus_tag="PC545_003481"
     CDS             723..1709
                     /locus_tag="PC545_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDF2458198.1"
                     /translation="MSKVKRLTDVWIYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMIILFALKSLQIYNYKLMEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANDTNDGDFKSSNVTEFFRASSESLILNIKY
                     KCGNQILEKNVCPIGIYLNKGAWFSPAYDCKSDKIILVHIDNIIDIKRTGESKKIHIN
                     LEEWLHEVYNNLYGNSSTTKKFGEIIYLNVLLTKEGVSKIKDSSWNLDMELNEDGSGI
                     ISTFIKVEDIDCYASILFFIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            1759..2481
                     /locus_tag="PC545_003482"
     CDS             1759..2481
                     /locus_tag="PC545_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HDF2458199.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEVEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIRDIIKAVSYMSNERRVLSIGMDCSEKENKEDLE
                     LGREFKINNDSIFKFVDLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKSYSEIADILFDRMEYIGEEFISKKGIVFVEGSPEFSIMELNDLISNIQSKVEESYE
                     VIFSLYIKENLNGNIRVGLLYN"
     gene            2510..4444
                     /locus_tag="PC545_003483"
     CDS             2510..4444
                     /locus_tag="PC545_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2458200.1"
                     /translation="MSENIRGTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFSMGKKSEKYINENCNDKDFIKKFMSIKKGDYFLYKAYQNIEEKVKCNVSIGIVEE
                     DFENGYELSHILGHTLPVKWMHNFDTVSNCKVYTSEFYKLNNNDISSYLEIIKSQDKK
                     LLRKRIEESENNKWYLKNYYYKNVDKCVDDEELFIQPINLEQLESLKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSALEGGYFFPVRWIEYEKENIDGNAWV
                     IFENNSIVEKYVKRFTNKMPLNTILYGPPGTGKTYNTKQMVYNLVSETSLRNKYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKQEDGILKDISIEACYNALKFEKKM
                     SFELEKDSLTLETIKDRKKKLVLENIEYSESFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFSLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPDYDLLSIVDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEKLIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            4437..5705
                     /locus_tag="PC545_003484"
     CDS             4437..5705
                     /locus_tag="PC545_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDF2458201.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENVILEILPKLSLSDNLVKDREILLQMLSICNKIPIT
                     MNEKIRLSLKNYNLLNFFVMYFIESMQTQMKKGIYFEYINKIENLNVMRGKILLSTYA
                     KEKGISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILSYFQNVD
                     LIYIDRKKLLDYKFYKNNDRFKDCYLLARLILLNLSMDNSQNNQEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKNEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITAYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEAKTVRLDDIKNTKNDLKKIIFNYKF"
BASE COUNT         2406 a          428 c          975 g         1913 t
ORIGIN      
        1 attgaatttg tatgtattat attgtataat gtaaaatgaa ttggaaaaat agttcgataa
       61 aataatttgt ttttataaaa ttactgttta agtttataga ttttgtataa aataaagtta
      121 tgttattaat ttttttagaa ataaaacttt tactaggaga cagatatgag aaaaaatagt
      181 attaatatag cactaactgc agcaattaca gcaatctatg cagttttaac attatcattg
      241 ggatttataa gttatggacc aatacaattt agggtagcag agattatgat gcttttggca
      301 tttttagata aaggatatat agtaggtctt acattaggtt gttttttagc aaatgtaata
      361 ggaccatatg gtgtacctga tataattttt ggaacattag ccacatttat aagtgcaagt
      421 atgatttata ttactagaaa gttaggtaaa cagaataaaa gtactttagt tatagcttct
      481 ttatggccaa ctgttataaa ttcgattgtg gttggtctaa tgttaaatgt attctttggg
      541 cttccattaa ttctatctat gatacaagta ggttttggag aatttgtagt agtgacaatt
      601 gtaggagtac catttttctg ctttttaatg aaaaaatata aaaatgtcat taatattaaa
      661 ttttaaatag tgtatagtaa tttaattaat tgagagtatt attagtgaaa agaggtgttt
      721 atttgtcgaa ggtaaaaaga ttgactgatg tttggatata tattaatgat aagcaaaagt
      781 ttacaataaa agagttgtca gaggaattta atctttcagc taaaacaata caacgatatt
      841 taaaagaatt aaataaaatg ggacttccaa ttcaagcaga acaaggtaga aatggaggtt
      901 atagggtgct aaataatagc tatatacctc ctgttatatt tacagaaaaa gaagttatga
      961 ttatactatt tgcacttaag tcacttcaga tttataatta taagttaatg gaaatagaaa
     1021 ttaattctat aatgagaaaa gtaagttatg aacgtaaaag cagtataaaa gtaggtatag
     1081 aaaatattaa aagatacatt ggctttgtag ctaatgatac taatgatgga gattttaaga
     1141 gtagtaatgt aactgagttt tttagagctt cttcagaatc tctaatttta aatattaagt
     1201 ataagtgtgg taatcagatt ttggaaaaaa acgtatgccc tataggtata tatttaaata
     1261 agggagcatg gttttcacca gcatatgatt gtaaaagtga taaaataata ttagttcata
     1321 tagataatat aattgatata aaaagaacag gagaaagtaa aaaaatacat ataaacttgg
     1381 aagaatggtt acatgaagta tataacaatt tatatggaaa tagttcaact actaaaaaat
     1441 ttggtgaaat tatttatcta aatgtattat taactaaaga aggtgtttct aaaataaagg
     1501 acagttcttg gaatttagat atggagctaa atgaagatgg tagtggaata attagtacat
     1561 tcataaaggt tgaagatata gattgttatg cttcgatttt attttttatt ggaaataatg
     1621 ctaaggttat agagccaaaa tttttgaata agctacttta tgataaggcc aatgagttaa
     1681 gatattttta tgaagaaaat atggtataat atttagaaat gttgaaatta aataattatt
     1741 aaggagatat acgttataat ggaaaatttt aataagaatg ctaaaataat tggtattgga
     1801 gctgaaggta ttaatataat aaatgaagta gaagaaaaaa taaaagcaaa tatggatata
     1861 gaaaaaataa acataaatca agagattgaa aaagaatatg ttagaagtct tttagatggt
     1921 gtagatatat tatttttgat atattcatct gaagataaac atattagaga tattataaaa
     1981 gctgtttcat atatgtcaaa cgaaagaaga gttctatcta taggaatgga ttgttcagag
     2041 aaagaaaata aagaagattt agagcttggg agagagttta aaatcaataa tgatagtata
     2101 tttaaatttg ttgatttaat gaatatcatg gtagagtcta tttctgattc ttgcatgata
     2161 aatatagata ttactgattt aaaagaagct attgtaggag ataaaggaat taagtattct
     2221 tttgaagagt ttgaagatac aaagtcatat agcgagatag ctgacatttt atttgacagg
     2281 atggaatata ttggtgagga atttataagt aagaaaggaa ttgtatttgt tgaaggaagt
     2341 ccagaatttt ctataatgga attgaatgat ttaataagta atattcagag caaggtagaa
     2401 gaaagctatg aagtaatatt ttctttatat ataaaggaaa atttaaatgg aaatattaga
     2461 gtaggcttat tgtataatta gtatatttta gatattaagg agagtgtata tgtctgaaaa
     2521 tataagaggt acagagtatt ggttaatgga agaatttttt tatcaggaaa cagacacacc
     2581 acagattttt agagaagaaa gcataattgc acttggattt gacataaaaa taaatttaaa
     2641 aaaagtattc agtatgggga aaaaatcaga aaagtatatt aatgagaatt gtaatgataa
     2701 agattttata aaaaagttta tgtcgattaa aaaaggagat tattttttat ataaagcata
     2761 tcaaaatata gaagaaaaag ttaaatgtaa tgtgtctatt ggcatagttg aagaagattt
     2821 tgaaaatgga tatgaattaa gtcatatttt agggcataca ctaccagtaa aatggatgca
     2881 taattttgat acagtttcaa attgtaaagt atatacaagt gaattttata aattaaataa
     2941 taatgatatt agctcatatt tagaaattat taagtcacaa gataagaaat tattgagaaa
     3001 aagaatagaa gaaagtgaaa ataataaatg gtatttaaaa aactattatt ataaaaatgt
     3061 agataagtgt gtagatgatg aagaattatt tattcagccc attaatctag aacaattaga
     3121 aagcctaaaa aatataaaga cagatgatta tattttattg tataatagcg agaaagtaat
     3181 ttgtgaaaat gaccatacaa tgaagagtct atttggcgta tgtcaagtta tggaagggtt
     3241 cgataaagag aaagtaagtg ctttggaagg tggatatttt tttcctgtta gatggataga
     3301 gtatgaaaag gagaatatag acggaaatgc atgggttata tttgaaaata atagtatagt
     3361 tgaaaagtat gtaaaaagat ttactaataa aatgccatta aatactatac tttatggacc
     3421 accaggaact ggtaaaacat acaatacaaa acagatggtt tacaatctag taagtgaaac
     3481 cagtctaaga aacaaatact atacaggtta cttagattat aaaaacaatg tagaattttg
     3541 tacatttcat caatcatatg gatatgagga atttatagag ggtcttaagt ctgatggtga
     3601 aggcaatttt aagcaagaag atgggatatt aaaggatata tctatagaag catgttataa
     3661 tgcattgaaa tttgaaaaaa agatgagctt tgagttggaa aaagatagct tgactttaga
     3721 aactataaaa gacaggaaaa agaaattagt attagaaaat atagaatata gtgagagttt
     3781 taattttgaa gaatcagaaa actatgtttt aataatagat gaaattaata gagggaatgt
     3841 atctaaaata tttggtgagc taataacatt attggaagat gataaaagac ttactaaatc
     3901 aaatcaaact attgtcaaac taccatattc aaaggaaaaa ttttctctac ctccaaattt
     3961 gtatatagtt ggaactatga acacatcaga taaatcaata gcacttttag atatagcttt
     4021 aagaagaagg tttaattttg aagaaattat gccagattat gatttattgt ctattgtaga
     4081 tggtatagat attagaaaaa tgctatatac aatcaataaa aggatagagt ttttatatga
     4141 tagagatcat gttattggac aagcttatct acttgctgta gaaaatatta atgatatagt
     4201 agtagtattt agaagtaaaa taataccact tcttcaagag tatttttatt atgattcaga
     4261 aaaagtaggt ttgattttag gaggaattgg aagaaatgaa aaagataaat acattgttta
     4321 taaagaaaaa cttatagctt ctgagttatt taaaaataat aatgcctcaa taatggagag
     4381 taaagttaac tattgtataa aacaaaacat atctagtgag gagctaaaaa atatatatga
     4441 gtaaaaatat atatgttaag gaaacctatg aatggataag ggtaggtaat ggtgaaaatg
     4501 agcttacaga aattgaatat gaaaaattgc ttaaatatct agagaacaat aatgatgtat
     4561 taaaaagtaa tataatagat attaaataca aaaaacttag atttataaac tatgtaggca
     4621 tcatatgttt tgaaaatgta atacttgaaa tacttcctaa attgtcatta agtgataatc
     4681 ttgtaaaaga tagagagata ctattacaga tgctttctat atgtaataaa attccaatta
     4741 caatgaatga aaaaataaga ttgagtttga aaaattataa tctcttaaat ttttttgtta
     4801 tgtatttcat tgagagtatg caaacacaga tgaaaaaagg tatttatttt gaatatataa
     4861 ataaaataga gaacttaaat gttatgaggg gtaaaatatt attaagtaca tatgcaaaag
     4921 aaaaaggcat ttctccaatg aaaataagat gtgaatatga tgagtatagt gaaaataatt
     4981 tcttgaatca agttcttaaa aaagcatgta tatctatttt atgtagaata aatgacaatt
     5041 caattcaagg caagattaaa aaaattttaa gctattttca aaatgtagat ttaatttata
     5101 tagatagaaa aaaactattg gattataaat tttataaaaa caatgataga tttaaagatt
     5161 gttatttact agctagactt atacttttaa atttgtctat ggataatagt caaaataatc
     5221 aagaggcatt ttctatatta tttgaaataa atactttgta tgaagaatat ataggaattt
     5281 taatcaaaag tatatgggac aattcattta gagaaacata tatacaagat aagagtaaat
     5341 ttcttttgaa aaatgaacaa acaggtaaga aaaactttaa tttaagaccg gatatagttt
     5401 tatatgactt aaaaaatgaa tatgaaatta taattgacac taaatggaaa gctattgaag
     5461 tagattcaaa tgttttttat aggtcaagtg atatatacca gatgtatgca tatataacag
     5521 catatgaaaa tgcaaaaaga tgtatattat tatatccttg tattcaaaaa gataagaatt
     5581 atagttcctg gaaattatca gaatcattta agggtaagtt tatagaagca aaaactgtga
     5641 ggttagatga tataaaaaac acaaaaaatg atttaaaaaa gataattttt aattataaat
     5701 tttaaaaata agaattttaa aa
//
LOCUS       DAMJVI010000164         5592 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.169, whole genome shotgun sequence.
ACCESSION   DAMJVI010000164 DAMJVI010000000
VERSION     DAMJVI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.169"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            240..1067
                     /locus_tag="PC545_003485"
     CDS             240..1067
                     /locus_tag="PC545_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HDF2458202.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPLMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGSGTVWLAPTQVMYEKLSFGAMPTNDNMNNKVSKQK"
     gene            2067..2530
                     /locus_tag="PC545_003486"
                     /pseudo
     CDS             2067..2530
                     /locus_tag="PC545_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(2797..3441)
                     /locus_tag="PC545_003487"
     CDS             complement(2797..3441)
                     /locus_tag="PC545_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458203.1"
                     /translation="MFGQSFQNCLVLIKENFKLSTLTNLICSIILLCMIPLIQGVANL
                     DRISTAICLENYVAIIGIVMLVPVFSLEQSKEIDEIVSSKALSQVIVQLIRILMSIIG
                     IVVLIIGFVNVLIYLGCNFPVVEYIIGSFASAMFIGSLGMFFSNLFNNTIIGYMISIG
                     YLILNMMTKGKYVGKFFIMSMSQGSFDEKYWLILGSVVLIIASLLIKPVKRKIR"
     gene            complement(3371..4660)
                     /locus_tag="PC545_003488"
     CDS             complement(3371..4660)
                     /locus_tag="PC545_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458204.1"
                     /translation="MNLFIKECKNILKSMIYYIFIVVLVFFYISQLGSSVSNDVSKNI
                     PNDGNPLVAPQKVENIEEYYSVHNQYPYGQKESMKTEKVIPNATINLIREYQKNTYTT
                     YPTGVVKYIKLDQNKTKTIEKYIEEITGKSISEINSLWKTDESIKIDYSKVSLERFEE
                     IMQRVDDILGGGSAYAFDKLQRFGVVPVTYDEAMKNYNYTIQKDKVSNGYARIFCDYI
                     GIVLSIFPVFVAVFMSLKDKRSRMREIIYSKQVSSCKIVLSRYFALVFMMILPVLILG
                     MKDLLPLIFFAKDKGISIDNLAFIKYTLWWILPTLMIVTAVGMSITILTDTPVAIIVQ
                     LIWWLYSINSTGLKGDYSTMGLMIRHNSVLNGDIIIQNFNQIMINRIVISLGALVLVL
                     LAIFLYEQKRRGNLDVWAKFSKLFSSNKRKLQIKHFN"
     gene            complement(4657..5529)
                     /locus_tag="PC545_003489"
     CDS             complement(4657..5529)
                     /locus_tag="PC545_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458205.1"
                     /translation="MEMKIAVSNLSKRFGKKQALDNINLEIDSGMFGLLGRNGAGKTT
                     FMRILATLLSKTEGSIHMCGVPIENTKEIRNMVGYLPQDFSMYPNMTVYKAMDYLAVL
                     SDIPLRKRRKIIAKLLSKVNLTNYQNVKVKSLSGGMNRRLGIAQALIHNPRVLIVDEP
                     TAGLDPEERIRFRNLLCEIANDRIVILSTHIVGDIEATCENVAILNSGKVIYNGSTEA
                     LAKLAEGKVYSIEVDKKDIENIKSRFIVIGMLTHGGKAILRIISDDKPFETAVNCNPT
                     IEDGYMLIMGGDNI"
BASE COUNT         2022 a          776 c          699 g         2095 t
ORIGIN      
        1 ataattttaa catgcccata aaatataata cccacaaatg ataaataata ctcaaaatgt
       61 ctattgaaat aactatacat ccttaaataa cttaacttaa atctaaacat ttcaaaaact
      121 tcttaaaaat atgtaaaaat ttgatgataa agtagcgatt ttttcaaaaa aaatgtaata
      181 tagagttata tcttataata ttttagagat aaattttatc atagtgggga gaaatttgta
      241 tggatttaag caaaaaagta aaaattttag aaaatgcatc agatggcaaa agcgaatttg
      301 agatattaga atttgaaaaa cttgagggag cttctagccc attaatggca atgggattat
      361 attttgctag agaagctggt ttgaaagcta gacaaattaa gataaaatta aataattcat
      421 caataaaaac agaagctggt gcattatact attataaagg aaatatatct tctgaaacta
      481 agattggtgg tgttggaggt ctttttaaaa aaggaatatc aggagcactt actaatgaat
      541 caatgatgaa acctgtatac aaaggtactg gagaagttta tttggaacct tcttttagac
      601 attatttatt tatgagtctt gataatgatt ctataattgt tgataaagga ttattctatt
      661 gttgctctga tacaatagaa gtaaaagcat ttacacaaaa gaacttctca tctgctttac
      721 ttggtggtga aggcttcttc caaatagaat taagtggtac tggtgtaatc gttctggaat
      781 gtgttgtacc tcaaagtgaa attgtagagt atgaattgaa aaatggtgaa gaattaaagg
      841 tagatggtaa ctttgcaata gcaagaacat ctggtgttaa tttttctgtt acaaaatcag
      901 ataaatcttt aattggttct gctttaaatg gtgaaggttt cttaaataca tttactggta
      961 gtggtacagt ttggctagct ccaactcaag taatgtatga aaaactaagt tttggagcaa
     1021 tgcctactaa tgataatatg aataataaag tttctaagca aaaatagttg ttttataaat
     1081 atttattaaa tcattattga ttttataaag tcactataaa ttttattaga ttattataaa
     1141 atcagagtgt ggaaatattt atgtgtattc tttaaataaa aatttccaca ttctgtaaag
     1201 ttattatact tgatatataa aattcaattt aggaacatag tctaacattt ataaacatat
     1261 taaggtaatg atttctcttg gtagttattc tcatatctta ttctttgatt atatttatct
     1321 tattcctagc ccttatgtca tattatattt cttatcttat tatttataat tagcattaac
     1381 ttatctaaaa tcttgtattt ttttaatagt aaagtttaca tctatattaa tttcataaaa
     1441 caatcaaggt agtcaagaga ttcaacttta tttgatttta aaaatttaag tgaatttgtt
     1501 cttatacaca cctttttaaa attatatctg tagaaaatat catttagagc tataattaaa
     1561 ttaacttatt aaattttaga aaatttatta aaaaatataa gttaaattaa tatttttaaa
     1621 gattcttttt tgtaactact agaacactaa tttagtagtt attgtatctc taaagtttaa
     1681 gcatggatag atatacattt gataatttgc ctataattta cctactactt ggattattgg
     1741 agcaaataca atatattaat aataagttcc taaaaccaat aaattaacta atacacataa
     1801 agatattgat gttgattata caactataag aaacggattt aacagaattt aatcaagcta
     1861 agaaattata tatatcgtga tgtttttgaa ttagaaggtg attcaaaatt agtttatagt
     1921 cttctgtgaa tccaataaaa agtacagggt acaagaattg ttagtaaaat ttttaaagat
     1981 tttatggata aatataaata gttttactta ttttctaaaa ctttaaaata ccattaatta
     2041 ccttaaatat tttaatagga ggacatatga atttaaataa agttaaattt actgaagaac
     2101 atgctattga agttactaat tggaaatatg aaggtaaata ttcaatatac aatctacctc
     2161 catgggagga aattaaaaag aagaattttt cactagcaaa agaagataaa agaaaaaatt
     2221 ttataagttt tataaatgac aataaagaat tgataggttt tataaactta ttagatgaag
     2281 gaagctctgt attctttggt attggttgaa accgaattat tgtggaatgg gaattggtaa
     2341 agaaataatt agcctaggat tacaagaatg tagaaataaa tatagtacta aaccaattgt
     2401 attaaatgta agaacctgga acatgagagc tgtaaaatgt tatgagtctc aaggatttaa
     2461 aatagttgag actaaggtac aaaaaacgca tttaggagat ggagaatttt ttgttatgag
     2521 gtatcaatag tttataacaa tagtcctatt gttaatacaa caattaaaat gaaaaaatac
     2581 atatgtgtag gtcatagcaa taagtgtctt tgaaatataa atttgagagc ttttgataaa
     2641 attcggtata catatatatt ttatactacg gatatataaa tctatctaca tacataaaga
     2701 gattgtaagt aggtttgtgt atataaaaat agatttatat ttcataaatg tactttttat
     2761 ttgcattacc tttattttat ctaagctctc attttattat ctaatcttcc tttttactgg
     2821 ttttattaag agagatgcaa ttatcaacac aacacttcca agtatcagcc aatatttctc
     2881 atcaaaacta ccttgactca tagacattat aaaaaatttt ccaacatact tgcctttagt
     2941 catcatattt aaaattaaat atcctataga tatcatataa cctatgattg tattattaaa
     3001 taaatttgag aaaaacattc ctaaacttcc tataaacata gctgatgcga aacttccaat
     3061 aatatactca actactggaa aattacatcc aagatatatt aaaacattta caaaacctat
     3121 gattaatacc actataccta taattgacat cagtatccta attaattgta caataacttg
     3181 agataatgct tttgaagata ctatctcatc aatttctttt gattgctcta gcgaaaatac
     3241 aggtaccaac attactatcc ctattattgc aacataattt tctaaacata tagctgttga
     3301 aattctgtct aaattagcta ctccttgtat tagaggaatc atacatagca atataattga
     3361 acatattaaa ttagttaaag tgcttaattt gaagttttct tttattagaa ctaaacagtt
     3421 ttgaaaactt tgcccaaaca tcaagattcc cccttctttt ttgctcatat aagaatatag
     3481 ctaacaaaac aagcaccaat gctcccaaac ttatcacaat tctattaatc ataatttgat
     3541 tgaagttttg tataataata tccccattta atacactatt atgtcttatc attagcccca
     3601 ttgttgaata atcacctttg agtcctgtag aatttatact gtaaagccac caaattaatt
     3661 gcacaataat tgctactggt gtatctgtta atatagtaat actcattcct acagctgtaa
     3721 ctatcataag agttggtagt atccaccata atgtatattt tataaatgct aagttatcaa
     3781 tagatatacc tttatcttta gcaaagaaaa tcaaaggaag taaatctttc atacctaaaa
     3841 ttaatacagg caatatcatc ataaagacaa gtgcaaaata tcttgataaa actattttgc
     3901 atgaagatac ttgttttgaa taaataatct ctctcattct tgaacgctta tctttaagtg
     3961 acataaaaac tgccacaaat actggaaata tacttaaaac tatacctata taatcgcaaa
     4021 aaattcttgc atacccatta ctaactttat ctttttgtat tgtatagttg taattcttca
     4081 tagcttcatc ataggtaact ggaactactc caaatctttg taacttgtca aaagcatatg
     4141 cagaccctcc tcctaaaatg tcgtctactc tttgcattat ttcttcaaat ctttcaagag
     4201 atactttaga ataatctatt tttatacttt catcagtttt ccaaagtgaa tttatttcgc
     4261 taatactttt accagttatt tcttctatat atttttctat ggtcttcgtt ttattttggt
     4321 ctaattttat atacttaact acccctgttg gatacgttgt atatgtgttt ttttgatatt
     4381 ctctaattag gtttatagtt gcatttggaa tgactttttc tgttttcata gattcttttt
     4441 gaccatatgg atattgattg tgtacagagt aatattcctc aatattctct actttttgtg
     4501 gagcaactaa tggatttcca tcattaggaa tatttttact tacatcattt gaaactgaac
     4561 ttccaagttg agaaatatag aaaaaaacta aaactacaat aaaaatataa tatatcatac
     4621 tctttaatat atttttacat tcttttataa atagattcat atattatctc ctcccattat
     4681 aagcatatag ccatcttcaa tagttggatt acaatttact gctgtctcaa aaggtttatc
     4741 atctgatata atcctaagta tagcctttcc accatgagtc aacattccta taactataaa
     4801 tctacttttt atattttcaa tatctttttt atctacttca atactatata ctttcccttc
     4861 agctagtttt gctaatgctt ctgttgagcc attgtatatg acttttccac tattcaatat
     4921 agctacgttt tcacatgtag cttctatatc tccaacaata tgtgtagata aaataacgat
     4981 tctatcattt gcaatttcgc atagtaaatt tctaaatctt atacgttctt ctgggtcaag
     5041 acctgctgtc ggctcatcta ctattaaaac tctgggatta tgaattaatg cttgtgctat
     5101 cccaagcctt ctattcatac ctccagataa agattttact ttcacatttt gataattagt
     5161 aaggtttact ttgcttaata actttgctat aatttttctt ctttttctta gtggtatatc
     5221 cgataaaact gctaaataat ccattgcttt atatactgtc atgttaggat acattgaaaa
     5281 gtcttgtggc aaataaccaa ccatatttct aatttcctta gtattctcaa taggaactcc
     5341 acacatatgt attgaacctt ctgtttttga taataaagtg gctaaaattc tcataaatgt
     5401 ggtttttcct gctccatttc ttcctaaaag accaaacata ccactgtcga tttcaagatt
     5461 tatattatca agagcttgtt ttttcccaaa tcttttacta agattggata ctgctatttt
     5521 catttccata tataaataca cccccagaaa caattttata gaattttatt tttataaatt
     5581 tttactgtat tt
//
LOCUS       DAMJVI010000165         5576 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.170, whole genome shotgun sequence.
ACCESSION   DAMJVI010000165 DAMJVI010000000
VERSION     DAMJVI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.170"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            157..1338
                     /locus_tag="PC545_003490"
     CDS             157..1338
                     /locus_tag="PC545_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2458206.1"
                     /translation="MNKKYIKAGNLIDGTGNSIMQNKGIIIDGDTIVEIEDIRDDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKIYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVNGNPVENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(1480..2397)
                     /locus_tag="PC545_003491"
     CDS             complement(1480..2397)
                     /locus_tag="PC545_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458207.1"
                     /translation="MENNKCREDFRFTQEYEEDYPNTNERYYENYQVADRYYNYPNKY
                     KEPKIKQCCCKKSMREALELLRYDALRPFVNFNQFAFISDFFIVGANLVGIDLSAPPK
                     DNLSGLDGTFERFSACNCDLIDIAGRVSYPIPVPLTLEGLINTIGTIPGVAELIALID
                     AVIPPTIDLGAILDAILATIIDFILAASTPLANVDLASLCNLKAVAFDITPADYEDFI
                     ASLGYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVIVLGNSNDSRIYFVCVDSIDYIA"
     gene            2650..3444
                     /locus_tag="PC545_003492"
     CDS             2650..3444
                     /locus_tag="PC545_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458208.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKVKTKKEEFFAVNNVS
                     FKVMEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINISGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEDIEESIENIVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGKIIASGTPQSLKEESGVNNLRDVFIKLARKEDI"
     gene            3445..5505
                     /locus_tag="PC545_003493"
     CDS             3445..5505
                     /locus_tag="PC545_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2458209.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKDTKNTSNDKGKIRVLDVDNYK
                     KSLEENKIDAYIKMEEKKSSINYQIYINSSQENSSNALKRIETVLDKYKRFTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMAFILVSGGISSQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGMAGIIMTQFMIISLPLLFAYYIKADFKEVFSLKVPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQNMEVAESLNKALFIKDSVLLNLLAIALLPALCEEMFFRGFIFSSF
                     SKSKDKNKSIKLAIICSGVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNSVAVVLNHYTTGNITNLYKFVEVDFSNLNVLKFALILLISLILIMLGLRVLDRK
                     SLRN"
BASE COUNT         2132 a          628 c          918 g         1898 t
ORIGIN      
        1 tattttttaa tgtttttgtg tataattata acaatttaat ttatattaat gcacttaaat
       61 ttgaaataga atgcttatag tggtaaaatt aattattaga atattagaaa taataaataa
      121 ataatacatt gtacttaaaa gggggagaaa tttaagatga acaaaaaata tattaaagca
      181 gggaatttaa ttgatggaac cggaaatagt attatgcaaa ataagggtat aattattgat
      241 ggagatacta tagttgaaat tgaagatata agagatgatt taaatggata tgatgtgtac
      301 gattacttag ataaaactgt tatgccaggc attatgaatt gtcatgttca cttgacaatg
      361 gagccagttg gaaatccaaa gatatattat gataatgtat cagatgtaga gcttgtagta
      421 aatacgataa aacaacttga tgcttatttg gattcaggag taacatatat tagaagctta
      481 ggttgtccca aatatgtgga tgtacaactt aaaaatctta tagacaaagg gtctatagat
      541 ggtcctggta tagttacttc aggtccagtt atatgtatga caggagggca tggatattat
      601 tttggtattg aaagtgatgg agtggatgaa tgtagaaaat cagccagaac agttttaaaa
      661 aatggagtag attgtgtaaa gataatggca actggaggtg taacaactga tggagttgag
      721 ccagggtcac cacaattaac tttggaggaa atgaaggctg ctgtagatga agctataaaa
      781 gcaggtaaaa taactgccac acatgcacaa ggaagaactg gtataaaaaa tgcagtgctt
      841 gcaggaatta cttctataga acatggtgtt tatttagatg atgaaataat tgatttgatg
      901 cttgaaagag gaacttactt agttccaact gtggctgcac catattttat tctaaatgca
      961 ggaaaagaaa gtggaataac tgaagctact cttagaaagt gtgagattgt tgccattcca
     1021 cataaagagt cattcctaaa agcatataaa aaaggaatta aaattgcagt tggttctgat
     1081 gcaggaactt cttataatga acatggaaaa acatattatg aaatgaaact aatgtcagat
     1141 tatggtatgg ataatatgga tataatagtt gctgctacaa aaacagcatc tgaactttta
     1201 agaattgata aaaattatgg aactctagaa aaaggaaaga aagcagatgt tatagttgta
     1261 aatggaaatc cagtagagaa tatagacgta cttgcagatg tgttagcagt atttaaactt
     1321 ggtaaaaaag ttagataaat agtataaaaa taaaaaagag tgtctaaaga aatttttaaa
     1381 taattataga cactcttttt tattagtaat aattaaatct tttatacttt agaaaataga
     1441 ttctatttta tatttagata taactcttta aaatcattct tatgcaatat aatctataga
     1501 atctacacat acaaagtata ttcttgaatc attagaattt ccaagtacta taacatcatt
     1561 tttcttacct agaacttcaa caccagttag aaccaaacta ccagctacta cagtaacttg
     1621 attatttata tttgacatat aaagattatc taggatacct ttattacaac agcaatcatc
     1681 acaatcgcag ttgcaattac attctttgta atgtttttta tcaagatagt aacctaaaga
     1741 tgctatgaaa tcttcataat ctgcaggtgt aatatcaaat gcaacagctt taagattaca
     1801 caatgatgct aaatctacgt ttgctaatgg agtagatgca gcaagtataa aatcaattat
     1861 agtagcaagt attgcatcta atatagcccc aaggtctatc gtaggaggaa taactgcatc
     1921 aataagtgca attaattcag ctactcctgg tatagttcct atagtattaa ttaatccctc
     1981 aagagttaaa gggactggaa taggataaga tactctacca gctatatcta ttaaatcaca
     2041 gttacaagca gaaaatcttt caaaagtacc atcaagtcca gataaattat cttttggagg
     2101 agctgaaaga tctataccta ccaaattagc acctactata aagaaatctg agataaaagc
     2161 aaattgatta aagtttacaa aaggtcttag agcatcatat cttagaagtt ctaatgcttc
     2221 tctcatactt tttttacaac aacattgttt tattttaggt tctttatatt tatttggata
     2281 attatagtat ctatcagcta cttgataatt ttcatagtat ctttcatttg tatttggata
     2341 atcttcctca tattcttgtg taaatctaaa gtcctctcta catttattat tttccactaa
     2401 atacactcct tttatatttt aatttaaatg tatagataat taagcttatc tatatagtaa
     2461 tattatattt ataatgtaaa aaaatgttac aaaagatatt ataaacgaat aaaaaataat
     2521 aatttgccag ataaacttgt aaatttttat gaataatcca ttctaaaaca aaaaactgtg
     2581 tataaaaaag gtttatatgt tatatttata taaatataac atatgaacga gaaaggagtg
     2641 gtttgataaa tgattgaggt agataaattg tgtaagtctt ttacaagggt tgtaaaagat
     2701 gataagaata aaagttcaat aagaaagcta aagaaagtaa aaactaaaaa agaagaattt
     2761 tttgctgtaa ataatgtttc atttaaagtt atggaaggtg aaattgttgg aatattaggt
     2821 ccaaatggtg caggaaagac tacattactt agaatgttag gtggcatatt aacaccaaca
     2881 tcaggaagta taaatatttc tggatatgat tattcaatag atagaaattc agcaaaaaaa
     2941 gaaataggtt atctctctgg aaatacaaaa ctatatggaa ggctttctcc gagagaactt
     3001 ctaacaactt ttgcaagtct atatgagatg tcaaaagaag atatagaaga atcaattgaa
     3061 aatattgtaa aaataatgga tatgagtgaa tttatagata atagaattga aaatctatca
     3121 actggtcaga ctcaaagaac atctatagct cgttgtttaa tacattctcc aaaagtatat
     3181 atttttgatg agccaacact aggtctggat gttttaagta gtgcatctat tatagatttt
     3241 atgaagaatg aaaaactaag gggaaaaact gttctttact caactcatta tatggaagaa
     3301 gcagaaactt tatgtgacaa gatttttatg attcacaatg ggaaaataat tgcatcagga
     3361 acaccacaga gtttaaaaga ggaatctgga gtaaataatt tgagagatgt attcataaaa
     3421 cttgccagaa aggaggacat ttaaatgaga agtaagattg taaaatacat atttaaaaaa
     3481 gaaatgttag acatattgag agataaaaag actttattta tgatgattgt tgtgccactt
     3541 cttttatatc ctttacttat gttattaatg attcaagtaa tgaatatgtc tgcaagtagt
     3601 atgaatagta aggacattaa tatagctttt aataataaac ctaataaggt tttagtatcc
     3661 atgattgaag atgataattt agaaaaagat actaaaaata cttcaaatga taaaggcaaa
     3721 ataagagtgt tagatgtaga taactataaa aagtcattag aagaaaataa aattgatgct
     3781 tatataaaga tggaagaaaa aaaatcatct ataaattatc aaatatatat aaattcttct
     3841 caagaaaatt cttctaatgc actaaaaaga attgaaactg ttttggataa atataaaaga
     3901 tttaccatag aaaaaacttt atctgaaaaa ggattagatg tacaagctac tttagaacca
     3961 ataacatata gcactgtaga tgttgcaaag acggaagaag tggcaggata ctttttaggt
     4021 cagatacttc catttatatt aataatagga gttttactag gttcaatata tccagcaata
     4081 gatgttatgg ctggagaaaa ggaaagaggt acattagaaa cattatttac actgccaata
     4141 tcaaacttag aactggttat gggaaaatat atggcagttt ctcttagtgc aatagtaaca
     4201 gcaattttaa acattttatc tatgggactg actatggcat ttattttagt tagtggaggt
     4261 attagtagcc aatttggctt tggaaacttt aattatggag acttagtctt accaattata
     4321 actactttaa tttgcatatg tcttttttca atggtagttt cagcaataag tatgtgtgta
     4381 tgttcattag caaaaagttt taaagatgct caaaactaca taacacctct tatgttaatt
     4441 attatgatac catcatatgt ttctatgata ccaaatatag tattagatag agtgacagct
     4501 gtaatacctg tagtaaatat atctttactt ataaaaagtg tattatcttt taaaagcgat
     4561 ttaaatttaa tagccttagt tttagcatca aatgtagcct ttgttatatt ggcaatcata
     4621 ctactgtcta agatgttcaa ttctgaggaa atactttttg gtaatagtaa gagtttctct
     4681 ttcttagaga aaagaagtaa tataaaaaaa gggtctatac caactgtaag tgatggtgtt
     4741 attttatatg cagtaggatt agttctattg atttatgttg gttctttagt tcaaattaaa
     4801 tttggtatgg caggtattat aatgacacaa ttcatgataa tttctttacc gttattattt
     4861 gcatactata ttaaagctga ttttaaagag gtatttagct tgaaagttcc caaattaaag
     4921 catgtgtttg ggagcattgt tctttggatt ggtggataca tacttattat gattataaca
     4981 cagctaattt tattcttatt tcctcaaaat atggaagttg ctgaaagttt aaataaggct
     5041 ctttttatca aagatagtgt tttactgaat ttactagcaa tagctttatt accagcttta
     5101 tgtgaagaaa tgttttttag gggatttata ttttcttcat ttagtaaatc aaaagataaa
     5161 aataagtcta tcaagctagc tataatatgc agtggggtac tatttggtat tatgcatatg
     5221 gattttatta gaataattcc aacatcaata ttaggtatta tatttgcata tagtgtgtat
     5281 aaatctggtt caatatttgt atcaatgtta ttgcattttt taaataatag tgtagcagtt
     5341 gtgttaaatc attatacaac tggaaatata accaacttat ataaatttgt tgaagttgat
     5401 ttttcgaact taaatgtact taaatttgcc ttaattcttc taataagtct tatattaatt
     5461 atgttaggat taagagtttt agatagaaag tctttaagaa attaagtata aagttattat
     5521 atagattaaa atattaataa gcactaaagt atatggatat atcttgtata taaaaa
//
LOCUS       DAMJVI010000166         5525 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.171, whole genome shotgun sequence.
ACCESSION   DAMJVI010000166 DAMJVI010000000
VERSION     DAMJVI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.171"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            50..166
                     /gene="rrf"
                     /locus_tag="PC545_003494"
     rRNA            50..166
                     /gene="rrf"
                     /locus_tag="PC545_003494"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(380..1564)
                     /locus_tag="PC545_003495"
     CDS             complement(380..1564)
                     /locus_tag="PC545_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2458210.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMESNGVKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCNYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMIKLVNAVPVFIDTKKENGFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYTGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKTIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSIDIDNMVKTYKSRRDLIKSKLDSIDNVGYVNPNGAFYIFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLDKLKEFVYKI
                     MA"
     gene            1937..4288
                     /locus_tag="PC545_003496"
     CDS             1937..4288
                     /locus_tag="PC545_003496"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HDF2458211.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDDELVNKMQNFAFEESYRETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDDLGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRHKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4310..4849
                     /gene="nrdG"
                     /locus_tag="PC545_003497"
     CDS             4310..4849
                     /gene="nrdG"
                     /locus_tag="PC545_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDF2458212.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEEIAKEAKINGLDVWSYTGF
                     TFEQLLDKKNRAYFKNLNLLKQIDILVDGKFIAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKQVFLVEQYMKDDLSIAE"
BASE COUNT         2151 a          625 c          888 g         1861 t
ORIGIN      
        1 ttctataaac ttattaaaaa cttaatagaa atattaagca taaagattat gtggttacaa
       61 tagcagagag gatacacctg ttcccattcc gaacacagaa gttaagctct ctagcgctga
      121 tggtacttgg tgggaaactg cctgggagag taggacgtag ccacgtaatt tttttgttat
      181 aataaattta gaatttaact tatatagttt aatttgataa tttgatgaaa aaaataaaag
      241 acttattgat ttttaaatct ataggtcttt tattttttat ctaacattta aattaagttg
      301 ttgaatatat cttatattac ttaccttata tattaattat cattaataca ttatatctaa
      361 ttactattca tctaaattat tatgccatta ttttatatac aaattctttt aatttatcta
      421 atccagataa gaaagtattt tcactgcaag cataggatat acgaatatat ttatccatac
      481 caaatgctat gccaggaaca actgcaacat tatattcttc taaaaattga ttacaaaatt
      541 ctatagaaaa actatcttta tattcaaatt tctcagatac tttagaaaga tctataaata
      601 tatagaatgc gccatttgga ttcacatatc ctacattatc aatagagtct agttttgatt
      661 taattaaatc tcttcttgat ttatatgttt taaccatatt gtctatgtca attgagcaat
      721 ctttcaaggc tccataggct atgtattgtg ctgttagact tgggtgagat ataagatgtc
      781 cttgtataga actcattgct ttagcaatcg ttttattaga tgcagtatat cctaatctta
      841 atccagtcat tgcagctgac ttagaaaaac catttatagt tattgttata tctttagctt
      901 cttcgcttaa tgatgcaatt gatgtaaatt caccagtgta gcatattttt tcatatattt
      961 catctgctaa aatatatatt ttattttgta tgcatacatc tactatttct attaattcat
     1021 cttttgtata aacactacca gtaggatttg aaggattgtt tattacaaga atttttgttt
     1081 tatctgttat agatttttca agttcttctt ttgttaactt aaatccattt tcttttttag
     1141 tatcaataaa aactggaaca gcattaacta acttaatcat ttcaggataa ctaacccaat
     1201 aaggctttgg taataagact tcatctcctt cgtctgttaa agctagtaaa gtatttgtta
     1261 ttgagttttt tgctccactt gatacaacta tttcgtctat tgaatagtta cagttatttt
     1321 cttcaataag ttttttacag atttcctctc taagtatttt taatcctgga actaaatcat
     1381 atttagtgta atctttattt aaagaatcaa taccataaga ttttgcgtta ttaggtacat
     1441 taaaatcggg ttctcctata ctaagattta ttactttaac tccattactt tccatttctt
     1501 taactttaga actaatacct attgtgtagg atggtgttat aaagtttagt ctttttgata
     1561 acataaaatg cctcctttta aattattatt tatattatac gacaaattta aagaatttac
     1621 ataaggaaat ttgtagatat atacattaat atatataata gcctacactt tttgcctaaa
     1681 attagtgtaa aaaaaagaga aaaaaatttt aactttcaaa gttgttagaa acgctactgt
     1741 tcaaaatatc cacatagtat ttgcaatgat ttgcgcaagt ttgactatta tgtacaaatt
     1801 tggtaatctt aatatagcaa ttattaagat aatcccttta acttagttga aggggatttt
     1861 tttagactta acatactaca tatagtggta tggggattaa taaaatacaa tatatagtaa
     1921 caggaggttt ttttaattgg ttgaattcgt aaaaaaaaga gatggaagag taattccatt
     1981 taatgaagat agaataacaa gagctatatt tttagcagca acgaatgttg cagaaagaga
     2041 aggaatagta cctgattata aattatctga gcaattaact caagaagtaa taaagttttt
     2101 aaatcataaa tactcagaat cagttccaag tgttgaagat atacaagatt ctgtagttaa
     2161 ggttcttata gaaactggcc atgcaaaaac tagtgaagaa tatataatat acagaactga
     2221 aagaagtaga ataagaaatt caaaaacaag gttgatgaag gcaatagagg aaattacctt
     2281 tgaagatgca gaagatgctg atataaaaag agaaaatgca aatattaatg gtaatacagc
     2341 aatgggaact atgttacaat atggaagcac tgtatctaaa gaattttgta aaacacatat
     2401 attaaaacca gagcattctt ttgctcatga taatggagat atacacatac atgatatgga
     2461 ttttttaaat atgggaacat taacttgttg tcaaatagat gttaaaaaac tattcaatgg
     2521 tggattttca acaggacatg gctttttaag agagccacaa gatataataa gttatggtgc
     2581 attagctgca attgctatac aaagcaatca aaacgatcaa catggaggtc aaagtatacc
     2641 atttttcgat tatggattgg ctgaaggtgt atataagaca tttaagaaat tctacatagg
     2701 aaatttagct aaagcattaa agttatttaa aggaattgaa aataatgatg tgataaagaa
     2761 catagtatat aatacagaga aagaaacaaa tcaaaaagtt ggtcttaaga gagatgaatt
     2821 gtatctaaac ttagaaaaag agaaattaat acagactttt gatatagatg atgaacttgt
     2881 aaataagatg caaaactttg catttgaaga atcgtataga gaaactgata aaaaaacata
     2941 tcaatctatg gaagcattta tacacaatct aaacacaatg cactctagag caggtgcaca
     3001 ggtaccattt tcaagtgtaa acttcggaac agatacttct gaagaaggaa gaatggtaac
     3061 taagaatctt ctattatctc aagaaagagg tttaggtaat ggagagacac caatttttcc
     3121 aattttaata tttaaggtca aagaaggcat aaatttaaat cctgaagacc ctaattatga
     3181 tttatttaaa ttatcatgca gagtatcagc taaaagacta ttccctaatt ttagtttttt
     3241 agatgcgcct tttaatgcaa aatattataa aaaaggtgaa ccagatacag aagctacata
     3301 tatgggttgt agaacaagag tacttagtaa tgtgtgtggt tcagaaacag ttagtggtag
     3361 aggaaacata tcttttacta cagtaaattt accaagattg ggtataaaac atggtattat
     3421 aaataatgaa aaggctaatt tagacggttt ctttgaagaa ttagatgaga agataaactt
     3481 aattatagaa caattattgg agcgccttga ggtacaagga aataaaaaga tgaagaattt
     3541 tccattctta atggggcaag gtgtatggaa aggttctgat gatttaggac cagaagatac
     3601 actaaaagaa gtaataaaac aaggaacatt gacaattgga tttataggtt tagcagagtg
     3661 ccttattgct cttataggta aacatcatgg agaaagtaaa gaagctcaag aattaggatt
     3721 aaaaatagtg tcacatatga gacataaaat ggatgaagca acggacaaat ataaattaaa
     3781 tttctcctta atgggtactc cagcagaagg tctttcaggt aggtttacta aaatagataa
     3841 aaaagtttat ggagaaatta aaggtatcac agataaagaa tattatacta attcattcca
     3901 tgtaccagtt tattacaata taagtgcata tgataaaatt gagatagaag ctccatacca
     3961 tgaattgact aatgcaggtc atataacata tgtagagtta gatggagatc catctgataa
     4021 tttagaagct tttgaaactg ttataaaagc tatgaaagat ttgggtatag gatatggaag
     4081 tataaatcat cctgtagata gagacccaat atgcggtttt tcaggtgtaa taacaagtaa
     4141 tatatgccct gtatgtggaa gaaatgaaga tgaaagtgat atcaaattcg aaagaataag
     4201 aagaataaca ggatatttag ttggtacagt tgatagattt aataatgcta aaaaagcaga
     4261 agtaagagat agagtaaaac atagatagtt ttatttgggg gaataagtta tgaaaataag
     4321 aatgtcatct actataagtt atgacagcat agttgatgga ccaggattaa gaatggtaat
     4381 atggacccaa ggatgtattc ataattgtaa agagtgccat aatcctcaga cacatgattt
     4441 atgtggagga ttttatatgg atacagaaga aattataaat aaagtcaaat cattgaaatt
     4501 acaaaaaggc attacgctat caggaggaga accattttta caaccagaac cattagaaga
     4561 aatagcaaaa gaagctaaaa taaatgggtt agatgtatgg tcatatactg gatttacgtt
     4621 tgaacaactg ttagataaaa aaaatagagc gtattttaaa aatttaaatt tactaaagca
     4681 aattgatatt ttagtggatg gaaaatttat agctgaaaaa aaagatataa gcttaaagtt
     4741 tagagggtca tcaaatcaaa ggataattga tgttcaaaaa agtttgaaat ataaacaagt
     4801 gtttttggtt gagcaatata tgaaggatga tttatctata gcagaataaa atataaataa
     4861 acaggattaa atatatgcat atatttaatc ctgtttattt tcatataata tttcaagctt
     4921 gtataatgta acaattaaac caaaagactc attgtatatt tgaaattaag ttttttatag
     4981 tttataggtg ttttatgtgg attacgaatg atatagtatt gatacattat ttaatagatt
     5041 cttatgccaa gaaacaatta atttatagaa attcttaatt gttacaatat aaatatctag
     5101 atatgttata agatactgat aaataattta taaagtttat taatctaata ttgtgtgtga
     5161 atattaagtg gtcaagtagt ctaaaaaatg aaaaagattt aaaaaagtgt tttaactaaa
     5221 cacataagtg ttaaattcat gtataagtat aggttagata cataagaaat aattatgtaa
     5281 tatctatctt tgtaaaaaaa tattaaattt tgtaatttgt ggataaatat gtttataatg
     5341 tggataatat ttaatgtaat aagttaaagc aatatgaatc aactgtttat aatgtggata
     5401 atattataaa tgtatgaata attgcagtat ttaaaaacaa aaaaagttaa aaaagttgtt
     5461 gactctaaaa gaaaaagttg gtatagtaat acttgtcctc aagaaaaggg caaacaaata
     5521 aagca
//
LOCUS       DAMJVI010000167         5421 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.172, whole genome shotgun sequence.
ACCESSION   DAMJVI010000167 DAMJVI010000000
VERSION     DAMJVI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.172"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            227..1063
                     /locus_tag="PC545_003498"
     CDS             227..1063
                     /locus_tag="PC545_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2458213.1"
                     /translation="MNIKKIFNNNVVVSSLEDGTEIIVTGAGVGFKKKVGDLIDENLI
                     SKKYFVQDDQRDKYNQILNKTSIEYFKISEEIIEKANEVLNTQVNDSIILALTSHIEF
                     AVQREKQGIKLPNLILNETKQLYREEFEFGLWAIDEIEKRIGIKLPEDEAGYIAIHII
                     NGLENSQKDEGINILEFSKQVIQIIEEVHGFKLDVNSLNYTRLITHLKFFVQRILRKE
                     TYNDSDIEDMYKLVNKNYNKPKVCTEEIAALVLDRFEYKISKEEELYLMIHINKITNS
                     GC"
     gene            1416..2993
                     /locus_tag="PC545_003499"
     CDS             1416..2993
                     /locus_tag="PC545_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDF2458214.1"
                     /translation="MKKNKLGLWNFFQMLGKTFMFPIALLSVSGMMLGLGAGFTDPKM
                     IEMVPFLGNEYVNLVLNFMLTIGLFAFNNLGALFAMAIPLGLLKKEKEFGAFSGLVGF
                     IAMHIGTNFYLTRADLLVPAEQMSTNGQAMIMGIQTYNTSVLGGIVAGLIVYAIYDKV
                     SNLKIPESLGFYSGPRLVPIVSLIVMSIVGLLIPIVWPPFFNAFQSLGRWISSAGPLG
                     YFSYAVAERVTIPFGLNHLVTSVFRFTPIGGTAVIDGETYFGTLNMFMAYVENNQIIP
                     LDLAGKMEQGKLMIQYGLAGAALAIYRTAKPENRKALKGLLISGVLTVIIGGISEPIE
                     FLFLFISPALFAFHTFMNGLANMVLPYLGVLMGFTGDLIAFISFGVLRGTATGWPIAV
                     LVAVLYFAIYYFVFKWAIIKFNIKTPGREDKEIKVSEGNGSDFKNLSTYKGQQMISAL
                     GGQSNILSLDNCVTRLRLKLQDVSLINEDAIKEAGGIAVVKLDEHTIQVIIGTQVYSL
                     RKQMDKAMEGHLEACND"
     gene            2986..4170
                     /locus_tag="PC545_003500"
     CDS             2986..4170
                     /locus_tag="PC545_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDF2458215.1"
                     /translation="MINFDKKVDRLGTYCTQWDYVEDRFGKKDLLPFTISDMDLEFPF
                     EITEVLQERLNHRVLGYSRWKHDDFRNSVTNWYIKRFNCSVDSNWIYYSPSVMYSISK
                     LIEMLSNEGDGILINTPAYNAFYDVITNNNRNIIKSHLVNRNGFYSIDFNDFEKKCKK
                     SKIFILCNPHNPIGRVWSELELVKMIGICKNNDVKIISDDIHMDIVYDNRMTPILNVA
                     KDYLDSVFICTSASKSFNIPALTGSYVIIPNEGIRNKFENITRYRDFVNSPAILAILA
                     TMVCYDDCEYWLDELLKYLKENLKYTIDYIKDNLEPLKLVMPEGCYFAWIDFSELKIS
                     GEKFQKLLMDIGEVAIMSGSVYGEEGEFYLRLNVACSREKLEDGLNRIKKTMDYIAKR
                     IL"
     gene            4188..4787
                     /locus_tag="PC545_003501"
     CDS             4188..4787
                     /locus_tag="PC545_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDF2458216.1"
                     /translation="MGYLKTMVDVMTTEMNAIRCLIDEAGIEYESIVSEIANCEGKVI
                     FMGVGKSAHIGKKLAATFASTGTPSFFVHATEAVHGDLGMIESKDITILISNSGNSME
                     VVNCIKYIKAIGSKTIAFTSNRNSVLAKECDYALIYPAKDEADHLNLAPTTSSTITLV
                     LGDSIACALSKSSNFGSSDFYKYHPGGSLGEKLKTANNG"
     gene            4811..5281
                     /locus_tag="PC545_003502"
     CDS             4811..5281
                     /locus_tag="PC545_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2458217.1"
                     /translation="MFKMFKKKSIKSPISGKVIELSKIQDGVFSQKIMGEGVAIDSTG
                     DIVYAPINGKIVVVAETKHAFVIELENGMELLIHVGLDTVNLKGEGFEVLVSMGEQVK
                     EGTPMLKINRPLIESKGISLITPVTITNHSEYNMNTCNVGNSVEGGKDTVIEFK"
BASE COUNT         2034 a          566 c         1033 g         1788 t
ORIGIN      
        1 gattttaaat aattaaatat acataagaaa taattaaata tctttcaatg aaaatatgat
       61 aaaatatgac aaattaattg atgacaataa aaaatatttt gttattgtag tattataggg
      121 taaattgcaa aatatatacg aaagtaaaat tttaaatatt gacattaaat taaatatact
      181 atactatgta agtgtgatga gagaattttt ttagagggga tttatagtga atataaaaaa
      241 gatatttaat aacaatgtag tagtaagctc cctagaagat ggcacagaaa taattgtcac
      301 tggtgctgga gtaggattta agaaaaaagt aggcgatttg atagatgaaa atttgatttc
      361 taagaaatat tttgttcaag acgatcaaag ggacaaatac aatcaaatac ttaataagac
      421 ctctattgaa tactttaaaa tttcagaaga aattatagaa aaggctaatg aagttttaaa
      481 tactcaagtg aatgattcaa taatattagc cttaactagt cacatagaat ttgctgtcca
      541 aagagaaaaa caaggtataa aattaccaaa cttaatattg aatgagacaa aacaacttta
      601 tagggaagaa tttgaatttg gattatgggc aattgatgaa attgaaaaaa ggataggaat
      661 aaaattgcct gaagatgaag caggatatat tgctattcac ataatcaatg gtttggaaaa
      721 ttctcaaaaa gatgaaggta ttaatatact agagttttca aaacaagtta ttcagataat
      781 tgaagaagta catggattta aattggatgt aaattcatta aattatacta gacttataac
      841 tcacttaaag ttttttgttc aaaggattct ccgaaaagaa acttacaatg atagtgatat
      901 tgaagatatg tataagttag tgaataaaaa ttacaataaa cctaaagtgt gtactgaaga
      961 aattgcagca ttggtattag acagatttga atataagatt agtaaagaag aagaattata
     1021 tttaatgatt cacataaata aaataacaaa cagtggttgt taggattgta actgataaag
     1081 caggcaaaac ctaagtcatt gagcttaact attagattaa gttttttgat ttaggttttt
     1141 ttattagata caatcaaaat acagacagtg acttttagga ttgtaactgg ttaagcaggc
     1201 aaaacctaaa tcattgaatt taactattag gttaagtttt ttgatttagg tttttttatt
     1261 agatacaatc aaatacagac agtgactttt aggattgtaa ctggttgagc aggcaaaacc
     1321 taaatcatta aacttaatta ttaaattaag ttttttgatt taggtttttc tataaatttg
     1381 attatgtttt tagataaaaa taggagggga aaattatgaa aaaaaataag ttaggtttat
     1441 ggaacttctt ccaaatgtta ggaaaaacgt ttatgttccc aatagcacta cttagtgttt
     1501 ctgggatgat gctaggactt ggggcaggtt ttacagaccc taagatgatt gaaatggttc
     1561 catttttagg gaatgaatat gttaacttag ttttaaactt catgttaaca ataggattat
     1621 ttgcatttaa caacttggga gcattatttg caatggcaat tccactagga ttattaaaaa
     1681 aagaaaaaga gtttggtgct ttttcaggat tagtaggatt tatagcaatg catataggta
     1741 caaattttta tttaacaaga gctgatttgt tagtaccagc agaacagatg tcaactaatg
     1801 gtcaagctat gattatgggt atacaaacat ataatacaag tgttttaggt ggtattgtag
     1861 caggattaat tgtttatgca atatatgata aagttagtaa tttaaagata ccagaatcat
     1921 taggattcta cagtggacca agattagttc caattgttag tttaatagtt atgagtatag
     1981 ttggattatt aatacctatt gtttggccac catttttcaa tgcattccaa agtttaggaa
     2041 gatggatttc ttcagcagga ccattaggat atttttcata tgcagtggca gaaagagtta
     2101 caattccatt tggactaaat catcttgtaa catccgtatt ccgttttaca ccaataggtg
     2161 gaactgctgt tattgatgga gaaacgtact ttggtacatt gaacatgttt atggcatatg
     2221 tagaaaataa tcagattata cctcttgatt tagctggtaa aatggaacaa ggtaaattga
     2281 tgattcaata tggattagct ggtgctgcat tggctatcta ccgtacagca aaaccagaaa
     2341 atagaaaggc acttaaagga cttttaatat caggggtgtt gacagttatt ataggtggta
     2401 taagtgaacc aatagagttt ctattcttat tcatatcacc agcattattt gctttccata
     2461 catttatgaa tggtctagca aacatggtac ttccatactt aggcgtgttg atgggattca
     2521 caggagattt aattgcattt ataagttttg gtgtgttaag aggaacagct acaggatggc
     2581 caatagcagt attagtagca gttttatatt ttgcaatata ctattttgta tttaagtggg
     2641 caattattaa gtttaatata aagaccccag gacgtgagga taaagaaata aaagtatctg
     2701 aaggaaatgg ttcagatttc aaaaatttgt ctacatataa aggacaacaa atgatatctg
     2761 ctttaggtgg tcaatctaat atattatcac tagataattg tgtaactaga ttgcgtttaa
     2821 aactacaaga tgtatcatta ataaatgagg atgcaattaa agaggctggt ggtatagcag
     2881 ttgttaaatt ggatgagcat acaatacaag taattatagg aactcaagtg tattctttaa
     2941 gaaagcagat ggataaagct atggaaggtc atttggaggc atgtaatgat taattttgat
     3001 aaaaaagtag accgattggg tacatattgt acccagtggg actatgtaga agatagattt
     3061 ggtaagaagg atttgcttcc ttttacaatt tcagatatgg atttagagtt tccatttgaa
     3121 ataacagagg tgttgcagga aaggttaaat catagagttt tgggatacag tagatggaaa
     3181 catgatgatt ttagaaattc agttacgaat tggtatatta aaagatttaa ttgtagcgtt
     3241 gatagtaact ggatatatta tagtccaagt gtaatgtatt caattagcaa acttattgag
     3301 atgttaagta atgaaggaga tggaatcctt ataaatactc ctgcatacaa tgctttttat
     3361 gatgtgataa caaataacaa tcgtaatatt ataaagtcac atcttgtaaa taggaatgga
     3421 ttctattcaa ttgattttaa tgattttgag aaaaagtgta aaaaatctaa gatatttatt
     3481 ttatgtaatc ctcacaatcc aattggtaga gtatggagtg agttagaact tgttaagatg
     3541 ataggaattt gtaagaataa tgatgtcaag ataatatcag atgatataca catggatatt
     3601 gtatatgaca atagaatgac acctatctta aatgttgcaa aggactattt agattctgta
     3661 ttcatttgta catcagcgtc taaatctttc aatataccag cattgactgg gtcatatgtg
     3721 attataccaa atgaaggtat tagaaataaa tttgaaaata tcactagata ccgtgatttt
     3781 gtaaattcgc cagcaattct agcaatatta gctacaatgg tctgttatga tgactgcgaa
     3841 tattggttag atgaactatt aaaatacctt aaagagaatc ttaaatatac gattgattat
     3901 ataaaagata atttagaacc attgaaattg gtcatgccag aaggctgtta ctttgcttgg
     3961 atagattttt ccgaacttaa aataagtggt gagaaattcc aaaaattatt gatggatatt
     4021 ggagaagttg ctattatgtc ggggtctgta tatggtgaag aaggtgagtt ttatttaagg
     4081 ttaaatgtgg catgttcaag agaaaaactt gaagatggtt taaataggat taaaaaaact
     4141 atggattata ttgcaaaaag aattttatag taggaaggga gatagttatg ggatatttaa
     4201 aaacaatggt tgatgttatg actactgaaa tgaatgccat aagatgttta atagatgaag
     4261 caggaataga atatgaaagt atagttagtg aaattgcaaa ttgtgaaggc aaagtaattt
     4321 ttatgggtgt aggaaaatct gcacatatag gtaagaaatt ggcagctaca tttgctagta
     4381 ctggtacacc tagctttttt gtacatgcaa cagaggcagt gcatggagat ttaggaatga
     4441 ttgaaagtaa ggatattaca attttaatat caaatagtgg aaattctatg gaagtggtaa
     4501 actgtataaa atatattaaa gcaataggtt ctaagacaat tgcatttact tcaaacagaa
     4561 attcagtact ggcaaaagag tgtgattatg ctttaatata tcctgcaaag gatgaagcag
     4621 accatttaaa tctagcacca acaacatcat ctacaattac tttggtttta ggagattcta
     4681 ttgcatgtgc attatctaag agtagtaatt ttggttcttc agatttttat aagtaccatc
     4741 caggaggaag tttaggtgag aagttaaaaa cagctaacaa tggataaaag caaaaaggag
     4801 agtagttttt atgttcaaaa tgtttaaaaa aaaatcaata aaatcaccca tatcaggaaa
     4861 agtaatagag ttatctaaaa tacaagatgg tgtattttct caaaaaatta tgggagaggg
     4921 tgtagctata gattctacag gagacattgt atatgctcca atcaatggaa agatagtagt
     4981 agtagcagag actaaacatg catttgtaat agaattagaa aatgggatgg aacttcttat
     5041 tcatgttggt ttagatactg taaatttaaa aggcgaaggt tttgaggtat tggtgagtat
     5101 gggagaacaa gtgaaggaag gaacaccaat gttgaaaatt aaccgtcctt tgattgaatc
     5161 aaagggtata tcacttatta ctccagttac aataactaat cattcagagt acaatatgaa
     5221 tacatgtaat gtaggcaata gtgttgaagg tgggaaagat actgtaattg agtttaaata
     5281 aagaactaaa atacaaggtt aatatttata aatacctata aacttatgtt ttataagatt
     5341 gattgtatag atttgttgta taaaaaagaa ctctttaatt ataaattaag gagttctttt
     5401 ttattttgta aatattgttt a
//
LOCUS       DAMJVI010000168         5334 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.173, whole genome shotgun sequence.
ACCESSION   DAMJVI010000168 DAMJVI010000000
VERSION     DAMJVI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.173"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(65..673)
                     /locus_tag="PC545_003503"
     CDS             complement(65..673)
                     /locus_tag="PC545_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDF2458218.1"
                     /translation="MVDNLIKYIHKSYVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFVAGYSVGA
                     TIAWRCSEDSFCDGIICCYGSRIRDYMDVIPKCPTLLVFAKHDSFDVDSISYQLSRKQ
                     NIQIEILDANHGFIDRYSNNYSDIQTKVFTIKEKSFLSKYLA"
     gene            complement(933..1478)
                     /locus_tag="PC545_003504"
     CDS             complement(933..1478)
                     /locus_tag="PC545_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HDF2458219.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(1596..3419)
                     /locus_tag="PC545_003505"
     CDS             complement(1596..3419)
                     /locus_tag="PC545_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458220.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVREMRSNLFLKFKNLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPIVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYERKSVNDFKEVNDRLLNISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGILASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEQEEQEVHNMFKSEINDNKDGNLNSHFVKGEIELKDVSFSYDSKKKILDNINLKI
                     KSGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDGKDIYSINRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANAHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(3432..5207)
                     /locus_tag="PC545_003506"
     CDS             complement(3432..5207)
                     /locus_tag="PC545_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458221.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGAIMIGITFLGAIFAIIRSVISARVSQHFSYDLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKIGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENQSF
                     EELNDELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRHLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLVDENEEVIEREESLDVGEKDLKFV
                     NCDIIFENVDFSYNKNTDEKILKNVSFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGRSIYSISEESLRARIGVVFQNSSVFGKSILENVVMGNRDICKEDVVKSC
                     EIANANQFITKLENTYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKEYISNLREKESKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFALQNLVSEDVN"
BASE COUNT         1784 a          932 c          470 g         2148 t
ORIGIN      
        1 caatagtgtc ataaaaactt ttcagattaa ttaaatcttt ctatatattt tataataatc
       61 taaatcatgc taaatattta gacagaaaac ttttttcttt aatagtaaat acttttgttt
      121 gaatatcaga ataattattt gaatatctat ctataaaacc atgatttgca tctaaaatct
      181 caatttggat attttgtttc ctagaaagtt ggtaagaaat agaatcaaca tcaaatgaat
      241 cgtgctttgc aaatactaat aaggtaggac attttggaat tacatccatg taatctcgaa
      301 tccgagagcc ataacaacaa attatgccat cacaaaaaga atcttcactg catctccatg
      361 caatagttgc tcccacacta tacccagcaa caaatacata tttatactct gattttaaat
      421 catttataag cttttcaact tttttataaa tatcaaaacc aacattattt ataaaaaaat
      481 catatgcctc ttggactttt gaatatggaa aaactctttt ttctcccaga agttcaggac
      541 aaaatacatc atatccatat ttataatact tttgacaaac atcttctata aagctattaa
      601 tcccatagat ttcatgtaaa acaacaatag ctacataact tttatgtata tactttatca
      661 gattatccac cacctttaca aaacagcaaa ttttttatac ttagtttaat atattcttct
      721 ttacatgtca atttactttt ctaaaatcaa atttattttt ggattaatca tttattttta
      781 aaagttgtgc attattacta ttaaattatt ctctattatt aaaaatataa tcgttagaaa
      841 taaaaaataa agtacaaata attaaaaaat atctcactca taactttaaa aattgtaagt
      901 gagatactcc taaaatctac tttatattaa atttattttc caaaatatct atttaataat
      961 cctaagaatg ctttaccatg tctagcttca tctttacaca tttcatgtac tgtatcatgt
     1021 atagcatcta atcctaattc ttttgctttc ttagctaatt ttaattttcc atcagttgca
     1081 ccatactcag catcaactct aactcttaag ttttcttttg tatctgcaac tacaacttct
     1141 ccaagaagtt ctgcaaattt agcagcatgt tcagcttctt caagagctat tctcttataa
     1201 gcttctccta cttctggata accttctcta tcagcttgtc tactcattgc taaatacatt
     1261 cctacttctg tacactcacc aacaaagtta gctcttaatc cttcgattat ttcttcatct
     1321 acaccttgag ctactcctat tctgtgctca tcagcccaat ttttttcgcc tttcatttct
     1381 tcaaatttat cagctccaac tttacatact ggacattgtg ctggtgcagc atctccttca
     1441 tgtatatatc cacatactgt acaaacaaat ttcttcataa ttactttcct cctaaatttt
     1501 atatatcaaa ttaaattttt tatttattaa gttagtctat atatacccgc accatatacc
     1561 tttaaacaat ttttttaaaa gttttttcta aatacttatt ttccctgtgt caataatatg
     1621 tttctataat aatgtccctt taattttatc aattctttat gatttcctga ttccacaatt
     1681 ctaccatttt ctaaaactat tatattatca caatttgata tggtacttaa tctatgagct
     1741 ataattatac ttgtctttcc ataagttaaa ttatttattg cttcttgaat tttagcttct
     1801 gttgctatat ctatgttact tgtagcctca tccaaaataa gtattggagc attttttaat
     1861 actgctctag caatacttat taattgtttt tgaccttcac ttaaatcttc acccctatcc
     1921 gataatacag tactatatcc ttcacgcatt ttaactataa atttatgtgc atttgctatt
     1981 ttactagcat atataacttc ttcatcagta gcctccagat ttccatacct tatattttcc
     2041 ataactgtat cattgaatat ataaccttct tgtggaacta ctccaaaaca agttctcaaa
     2101 tagtttctgt taatagagta tatatcctta ccatctaaaa atatacttcc ttcatctata
     2161 tgataaaaat ttgtaattag atttataata gttgtttttc cacttcccgt ctctcctatg
     2221 atggcatttg atgtgccaga tttgattttt aggtttatat tatctaaaat tttctttttt
     2281 gaatcataag aaaaacttac atctttaagc tcaatttctc cctttacaaa atgagagttt
     2341 aaattaccat ctttattatc gttaatctca cttttaaaca tgttatgcac ttcttgctct
     2401 tcttgttcat ctataatttc aaaaactctt tcacatccag ctatggctga aagtaatgta
     2461 ttaaatagat ttgctaattc atttaaaggt cttgtgaatt gtcttacata acttaaaaaa
     2521 ctagctataa cccctatggt tataatatct ttagatgcaa gaatgcctcc tataatacat
     2581 atagttgcaa atcctatatt actaatcaca ttcataatag gcattaaaag agaagtgtaa
     2641 atttgcgatt taagggaaat atttagaagt ctatcattga cttctttaaa atcatttaca
     2701 gattttcttt cataattaaa agattttact acatttatgt ttgatatcat ttcttcaata
     2761 tgtgcattta gttttcccaa ctcaacttgt tgttgcttaa aaaaaactct agttctcttt
     2821 gcaataaaat tactcacaaa cattactatc ggtattaata ttacactgca aaaggttaaa
     2881 attgggctta atacaaacat cataataaaa gtcatcaata tgtttaagat tcctgtaatt
     2941 aactgtacta atgaatttga tattcctgta ctgatattat cgacatcatt tgtcactcta
     3001 ctcataatat ctccagtaga acttttatca aaaaaggtta ttggtagatt tttaaatttt
     3061 aaaaataaat tacttctcat ttctctgact atactttgag ataatctagc tgtacatatt
     3121 cccttagaca gtttaactga aaactcaatt atataaaatg aaagtaaaat aattattcct
     3181 tttaagaaaa tggattttga ataagtatcg atatctatta tgtcaactac ctttccaata
     3241 gtatatggta ttacagttga tataattgaa tcaattatta ctagcaaaaa aataaatcta
     3301 gtaatcttct tatctttttt aaagtacata aaaattcttt taaacgtttt tttaaaatct
     3361 ttaggctttt cttttttctt taatctactg accttctgat ttccagtact taaattcaat
     3421 atacttcctc cttaatttac atcttcactt actaaatttt gcaacgcaaa aattttttta
     3481 taaattggag aactaaccag taaatcttca tgtgtagcaa atccaaccac ctcaccctca
     3541 tccaagacta aaatcttatc acagtcaatt atagaactaa tcttttgtga aatagttatt
     3601 acagtacatt tcttactttc cttttctctt aaattactta tatactcttt tatattcttt
     3661 cttataattg tctctgtatt aatatccatt gcactaaaac aatcatcaag aattaatatt
     3721 tgtggttttt tggctatagc tctagctata gtaattcttt gtctctgtcc tcctgataaa
     3781 tttttacccc tttggaataa aacttcatca taagtatttt cgagttttgt tataaattga
     3841 tttgcatttg ctatttcaca tgattttaca acatcctctt tgcatatatc tctatttccc
     3901 ataactacat tctccaatat actttttcca aacacactac tattttgaaa tactacacct
     3961 attcttgccc ttaaactttc ttcagaaata ctgtatatac tacgtcctcc aatcttaata
     4021 tcacctttat caattatata taatttttga attaaattta ctaaagaagt ctttcctgaa
     4081 ccagtttgac caatcacacc aatattttga ccttcttcta ttttaaagga tacgtttttt
     4141 aatatttttt catctgtatt tttgttatac gaaaaatcta cattttcaaa aattatatca
     4201 cagtttacaa actttaaatc tttttctcca acatctaaac tttcttctct ctcaattact
     4261 tcttcattct catcaaccaa aacctcactt attctctcat aagatgcttt tgccctaaca
     4321 aaaacattaa aaacatgtga aatcattata agagatgtta atattctagt catatagttt
     4381 acataagcaa ctataatccc aattttaatt tctcctgaat aaattaaatg ccttccaatc
     4441 agtaatatta ttattatgga aacattaatg attattgtaa ttgtcggtct aaaaaatgac
     4501 atcatagttg tagcttttct actattttta aaaagttcat catttaactc ttcaaagctt
     4561 tggttctcat aatcgtatct acaaaatagt ttaactactt ttattccact taaaaactgt
     4621 cttaattttg aatttacttt atctgtactt tcttgtacat ttttaaataa tctataccct
     4681 atcttcatat taagcaatat tataataaat ataattaaaa ctgcaattag aataattatt
     4741 gacatcttta aatttaattt tatagctata attaaacttc ctatacacat tattggtgac
     4801 tttataaaaa ctctcatcat tccattaacg aattgttgca tttgattgat atcattagta
     4861 attcttgtta ttaatgaagc tctaccaaat ttatctattt gttttaatga gtatgtattt
     4921 atcttttcaa atacatcctg tcttaaatca taagaaaaat gctgagaaac tcttgccgag
     4981 attacacttc ttattattgc aaatattgca cctaaaaaag ttatacctat cattatagct
     5041 cctgttgata gtatgtagtc taagttctta tttgaaaccc ctatatctat tatttttgcc
     5101 attatagttg gttgagctaa atcacaaaaa gcttccaatg ttaaaaaaaa tactgctgtt
     5161 agaaataata ctatatattt ttttatgtat tttttaaaat acctcattca atcatccctt
     5221 catagaattt tctcattacc aaaagtatat cataatggct aatttaattg attatatcaa
     5281 ttataaatat ttgatttatt attaattttt ataccatata ttttataaat tatt
//
LOCUS       DAMJVI010000169         5313 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.174, whole genome shotgun sequence.
ACCESSION   DAMJVI010000169 DAMJVI010000000
VERSION     DAMJVI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.174"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(323..523)
                     /locus_tag="PC545_003507"
     CDS             complement(323..523)
                     /locus_tag="PC545_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2458222.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
     gene            complement(748..924)
                     /locus_tag="PC545_003508"
     CDS             complement(748..924)
                     /locus_tag="PC545_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458223.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGEKVVE
                     SCLHQVMVLVSKYN"
     regulatory      complement(978..1067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(1247..1447)
                     /locus_tag="PC545_003509"
     CDS             complement(1247..1447)
                     /locus_tag="PC545_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458224.1"
                     /translation="MPKFTCNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     gene            complement(1449..1799)
                     /locus_tag="PC545_003510"
     CDS             complement(1449..1799)
                     /locus_tag="PC545_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HDF2458225.1"
                     /translation="MIQKKFFMGFCGFLGFLSLRYFSSGNVADLTYIGFFAFFSNFII
                     SKINGDKADERYVQDEKAAMAFTGQLAIIELFILWCITIVSRNIELMCVLLSITYAIT
                     LNVYAIKLYILEEK"
     gene            1961..2150
                     /locus_tag="PC545_003511"
                     /pseudo
     CDS             1961..2150
                     /locus_tag="PC545_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890498.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2627..2797)
                     /locus_tag="PC545_003512"
     CDS             complement(2627..2797)
                     /locus_tag="PC545_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458226.1"
                     /translation="MHVYQPKTSEYLLNTYAKSYLSSIVFLFDYITKNESGYAFFIID
                     FPFLSLDIFYKF"
     gene            complement(3186..3680)
                     /locus_tag="PC545_003513"
     CDS             complement(3186..3680)
                     /locus_tag="PC545_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2458227.1"
                     /translation="MKNKKGFTLVELLVVIAIIGILAIVALPALFKNIEKAKIAKLEA
                     DISAIKSASLSYYADESKYTDGGMISWVKKDGKIIINGGFKDDPLADKIENLGMPYNG
                     SYLLMSSPGHEKYLELSILPEGEISKSGLDKLKSDYGSSIDIMNEQNKIDIVIKFLND
                     KSNT"
     gene            4485..4862
                     /locus_tag="PC545_003514"
     CDS             4485..4862
                     /locus_tag="PC545_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2458228.1"
                     /translation="MKEEILIEKLLRVELIVMEYLWKKDTLMPKKKIVKEIKQIYGWH
                     KSTIKILLKRLVDKRYLARYIINFQSYYKIIIDNKEYYTFKKKVLKSSKSIKIMHSLT
                     TIHKNVSKEKLDLLDEYYRNLKE"
     gene            4967..5313
                     /locus_tag="PC545_003515"
                     /pseudo
     CDS             4967..5313
                     /locus_tag="PC545_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430377.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
BASE COUNT         2139 a          637 c          610 g         1927 t
ORIGIN      
        1 taattgcttt ttattaacaa attactaaaa gtattatatc agaattgaat acaaaagaaa
       61 gagtatgtat ttatgagata gttgaaaatt ttgatgtgcc acaactaaaa ttattatatt
      121 actatatatt ctaattaaaa gagtacttgt aggtttaagt acatacttta taaatatatt
      181 tttgtcagat ttattttata aaaagtattt agactaagta ttatttttaa gtattttttt
      241 gttgaagtta ttttttcctt ttttacaaaa tgcgtaaata aaaaaaagac tcttaaaaga
      301 gtctttccga aaaatctata tattataata tagttatatt ttctgcttga ggacctctag
      361 cgcctttaac tatatcaaag cttacttttt gaccttcttc taatgattta aatcctgaag
      421 tttgtatagc tgagaaatga gcaaatacgt catctccact ttccattgat ataaatccaa
      481 atcctttttc attattaaac cattttacta ttccattatt cattaaaaat actcctatta
      541 acctatatca agatatactt gatttacttg tgtattacca caaatactat tgtatcatag
      601 attaacataa atgtctattt aattataatt tttagttaac atttttattt attcttaata
      661 tgaaaataaa tttaaacatt ttatattacc aatatattaa tgtaaatgta gataaaacta
      721 aaaagggttt tctagaaaaa aatctgtcta attatactta cttaccaata ccatcacttg
      781 atgcaagcaa gattccacaa ctttttctcc gtactctaat ataaaatcat ctaactgtgt
      841 atctataaaa tcacaataat catatgaact accaatataa gcagttaaca tatgtgtaca
      901 tttatccatt ttatctctaa acataatgta tttatccccc taaaaaactt tcatgaaaaa
      961 caaaaagaaa atatcttttt ggcaactggc tgacataact tattttataa gcgttagtcc
     1021 ctatagcttt gcgtcactaa atttctctag ttttgccgat ttagttttat tctacagatt
     1081 aataatacaa tagaattagt tattattcaa catatctagc tgaaaaatta ataaatttat
     1141 atatgaacgt taaattatct gaaaaaataa aaagcccctc tgtcaggagt gacttaaaaa
     1201 ttgaatttaa gtttgtattt aaaacacatt tttaataatg caaaaactaa tcaaaaataa
     1261 acaattcatc aataggtgta tctactattt ttgatatatc tattgctaat ttcaaagaag
     1321 gattatactt tcctgcttct aaacgcataa tggtttctct tctaactcct actttttctg
     1381 ctaattgttc ttgagttaat tcttttaatt gtctatattt ttttaggtta caagtgaatt
     1441 taggcatatt atttttcctc taaaatataa agttttatag cataaacatt taaagtgatt
     1501 gcatatgtaa ttgatagtaa aacacacatt aattctatat tcctagacac tatagtaata
     1561 caccataaaa tgaataattc tataatagca agctgtcctg taaatgccat agctgctttt
     1621 tcatcttgta catacctttc atctgcttta tcaccattta tttttgatat aataaagttt
     1681 gagaaaaaag caaaaaatcc aatatatgta aggtcagcaa cattgccaga tgaaaaatat
     1741 ctaagactta aaaatccaag gaaaccacaa aaacccataa aaaatttttt ctgaatcatt
     1801 ttttatcctc cttagtaatt ttaaaagaga tatatttata tctttatgat acaaatatat
     1861 ctcttttata ttcttttgtc aatagaaaat cagtattata caatatttgt actgcaccat
     1921 aattgttaga gcaaaaatct actgcttgag atgtttatgt atgactaaat atgattttaa
     1981 tttcaaatta aaagtagcta aattttattt gaatagagaa ggaagagata ttttaatagt
     2041 aaaacaatat ggagtttata accattctca agttgaaaga tgggttaata tattaggaga
     2101 gtgtgcacta aaaataaaaa aaattttcta tactatatgg ttcaagttag atgttataaa
     2161 ctatatgatg ataactaaat atagtgtaca ataaatcgct aagtattttg gaataaacaa
     2221 tagcacagtt atagcaacat gaaaataaaa atttttacat agtggtgttg atgccttttc
     2281 taaaatgaaa ggaagaccat aatgaataaa aacaaaaaca ctaaagaaaa aataaattaa
     2341 caagagaaca agaattagaa tatgaaaata aatttttaaa agatgagctt gagtttttaa
     2401 aaaagatgtg tgcctcaggg aagccacatt aaaaaacagt ggtatcatta ttaatcaaaa
     2461 gaaagttaga tgtattatca aaaaattaga acttaaatgc atagcatttt ttcataaatc
     2521 acataaatat aactcctata aatgtataat tggaaaagta aaaaataata aaacaaatag
     2581 aagatttaaa atatcaatag catatcagaa aataacaaat gatatgctag aatttataaa
     2641 atatatctaa tgataaaaat ggaaaatcaa ttataaaaaa ggcatatccc gattcatttt
     2701 ttgttatata gtcaaataag aatactatag atgaaagata cgattttgca tatgtattaa
     2761 gtaagtattc tgatgttttt ggttgataca catgcaataa agaagttgtg tttgcagata
     2821 aattagcaaa actatagtat aagttgcaac aataaagaaa atgaaaatta tcatactaaa
     2881 gcataaggca tgttacaatt ttattagata ggacattaac atacattaaa gtacttttta
     2941 ttataaagag tacttatttt tttgaaattc attaatgtat aaactatcaa gaatattact
     3001 caacataatt taaaaacaac ttgaataggt atttttaaat tatgtgatat aataaaaaat
     3061 agaagtgtat gccaatacac ttctgtaatt ataactaagc attctattct tgaggagtgc
     3121 ttttttcatt tctccaaaca agattgttta tttcatcagc aattattaaa taaaatttat
     3181 tgtatttaag tattcgattt atcatttaaa aattttatta caatatctat tttgttttgc
     3241 tcgttcatta tgtctattga acttccataa tcacttttta atttatctag accacttttg
     3301 cttatttctc cttctggaag tatacttaat tctagatatt tttcatgacc aggagatgac
     3361 attaatagat atgaaccatt ataaggcata cctaaatttt ctattttatc tgctaatggg
     3421 tcatctttaa agccaccatt tattattatt tttccatctt tctttaccca tgatatcatt
     3481 cctccatcag tatattttga ttcatctgca tagtagctaa gagacgcact ttttattgca
     3541 cttatatcag cttcaagttt agctatcttt gctttttcta tatttttaaa taatgctgga
     3601 agtgctacta ttgccaatat tcctattata gcaattacta ctaataattc cactagagta
     3661 aatccttttt tatttttcat ttatttcccc tccatataaa attaataata tataaatttt
     3721 tattttgttg atttctactt tctatatttt accatatata gacaaaaaaa tcactttcta
     3781 tgacaaatat actattattt tacaatttat ggaaatgatt taaaatgaca tttcttgtta
     3841 gaaaagatat aagaaatgga ttaaaataaa gaaaactaca gtcatatcaa tgtcaataat
     3901 attatttaaa agcagatgaa aataaaaaga ggactcagaa aataatgtct atttaattat
     3961 acttacttac caataccata acttgatata aacaagatta tactacattt tttttatgct
     4021 ctagtataaa atcatctaac tgtgtatcta taaaatcaaa ataattatat gaactatcag
     4081 tataataagt caaaatatgt atacatgaat acatatttta aaagatatat attattacat
     4141 acctaaaaaa ctttcataaa aacaaaaata aaatatgctt ttagtaactt gttaacataa
     4201 cttataattt attttatagg cattagccta tatagctttg tgctactaaa tttctttgat
     4261 tctagcgatt tagtgctatt ctacaactaa aataatacaa tagagctatt tattgtttgg
     4321 tagcattgtt tgcaaattaa acaaatttat atatatgaat attattttac gtttaaaaaa
     4381 acagactaaa tatgtagaat atttggtaaa acttacctct agtttttgtc ttaaaaagct
     4441 cctattatta atttaagtta gaagaattaa ggaagtgaca taaattgaaa gaagaaatat
     4501 taatagaaaa attattaaga gtagaattaa tagtaatgga gtatttatgg aaaaaagaca
     4561 ctctaatgcc taaaaaaaaa attgtaaaag aaataaaaca gatttatgga tggcataaaa
     4621 gtacaataaa aatactacta aaaaggctag ttgataaaag gtatttggct agatatatta
     4681 taaattttca atcttattat aaaataataa tagataataa agaatattat acttttaaga
     4741 aaaaggtact aaaatctagt aaaagcataa aaataatgca ttcacttacc actatacata
     4801 aaaatgtaag taaagagaag ttagacctat tagatgagta ttatagaaac ttaaaagaat
     4861 gatatagtaa aaatcgaatg taattattta ccttaattcc tttgtttgta aaactttatt
     4921 gatatagaat ttgacatata cataaattgg taaagggatg tatactatga aaattagtaa
     4981 attaccagaa gcggaattaa aagttatgag atatatttgg gaatcagaaa aggtattaat
     5041 accaagagaa atcaccaagt ctatggaaca aaaatatgaa tggaaagata caactatatt
     5101 tacagtatta aaaagattac taaagaagag agtttttaga agcggagaaa atagataagt
     5161 gtacacacta taatgtatta gtaaaaaaaa ataatatcag agattttaaa caaaagaatt
     5221 tttaaagaat acacataaaa attcaatatt aagtttaatg tcagcactat gtagtaagga
     5281 tgatgaagta gatggtgata ttttaaattt att
//
LOCUS       DAMJVI010000170         5161 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.175, whole genome shotgun sequence.
ACCESSION   DAMJVI010000170 DAMJVI010000000
VERSION     DAMJVI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.175"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            63..1835
                     /locus_tag="PC545_003516"
     CDS             63..1835
                     /locus_tag="PC545_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458229.1"
                     /translation="MENGKFQFFKGVRNCFNMIYLHDKRYLFISMFMAIFQGIVPSIS
                     LLVSQNIINNMQTFSKSVKEILVLLIMYLIINIIPSIFQSFYGYYRIKFNKNFEKHIE
                     TMMLEKSLTLSLKDFEDDNTYDMINRAQSQKGFNITILSENLISIIRESVSTLSLIVV
                     LSQFQLWLIIVVIVIPVLKSLYSIKLGKEQYDVSVNRTWKERKAWYIDYLLSKGYAYK
                     EIMVFRIGSKLINHYKKLKEDIISQDMNIAKKNFIATLLCTVLDSIVSAGIIGFIIIQ
                     GILKKILIGDATTYIQAVQSIKDNVESIFSLFSSVIQDLFYTDLLFKFLDYNCKEDSI
                     EHIKIFEKIGDINKIELINISYKYNHSSEYSLKNINLTLNKNDPIAILGKNGSGKTTL
                     LKILLGFYDDYEGDILINGKNLKDIEKDEYIRKVSCMFQDYIKYEATLKENVAMGDVY
                     KSNLEEKVKSKLKETKLKPSIYEEDGLETILGTWFGKKQLSMGEWQKVAISRTLMKDA
                     DMYIFDEPDSSLDSEAGNELLSLYKKTFENKIGIFITHHIQHAKKITNNIVVLENGKL
                     LESGRHDELMDKKGVYFNLFCNCK"
     gene            1857..3278
                     /gene="ccpM"
                     /locus_tag="PC545_003517"
     CDS             1857..3278
                     /gene="ccpM"
                     /locus_tag="PC545_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich peptide radical SAM maturase CcpM"
                     /protein_id="HDF2458230.1"
                     /translation="MNQKPFIHTFKAGKSYFIYDVNTDKILKVNAAVYNYLNKIEHNL
                     EKESDWNIEIEDEINTLINYGFLKNKRVSKTMHPETKYIKYHIENNLSSIILQVTQNC
                     NLRCDYCIYSGSYSNRVHTNKRMNIDVAKRSIDYLIKNSSQLNEVSVGFYGGEPLLEF
                     DLIKQIVEYVKVVGEGKEIYFNLTTNATLFNEEIIEFFVKNNITTMISLDGGKEIHDK
                     SRKFANTGEGSHDVVLKNVRYIKNKYPEYYKKNITFNTVINSENDYISIDDFISKNET
                     LKDSNFLASTLNNNYSDKEIVSSDEYILGKSYDRFLTLLNKLKFIKDESMSPLSRIER
                     ALLDDFRYEKNIEGRLELPDVWHHGGPCIPGAFRIFVTTDGEFFPCERVSESSKMGMI
                     GNLDSGVDIEKAEKLLNIGKLYEEQCSDCWAYSYCDICIAKIDDTKCNENEIKPHDCD
                     RIRHSTENNMKDYCVLKSLGYQY"
     gene            3307..4377
                     /locus_tag="PC545_003518"
     CDS             3307..4377
                     /locus_tag="PC545_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04066 family peptide maturation system
                     protein"
                     /protein_id="HDF2458231.1"
                     /translation="MEKVLIYPFNYNFFTILNHCNKISDIEINSLVSPSGWGLCNKEY
                     NVNNIITKVTNDFQSELKKNSLVWFVDSKENLDLEKFIIPKLKVALKEGKKILYTRDD
                     LNKISRYISHYEFKKINEWNLEKYEIRDIEGISQINTPIIFICGLYGGLDTFDVNIRI
                     GEELRKRGFSILQFGPKKESYLFKMIPIPEFMFDDSISSKTKIINFNHFIKDKEVEAK
                     PDLIIIEVPGELFPFSRKMVGNFGIRAFEISNAVINDCGILCIPSSIYDREYCEELKY
                     FIKGRHGINIDYFSIEKKVIDTYETEVKQKFSYMSLDENFFVEDIEDYEKTFFIHDNK
                     QLLELVDNIVNQLKEYAKVISI"
     gene            4393..4644
                     /locus_tag="PC545_003519"
     CDS             4393..4644
                     /locus_tag="PC545_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide maturation system acyl carrier-related
                     protein"
                     /protein_id="HDF2458232.1"
                     /translation="MNYTEKELLLENIFFDVTGINFNKNNNLKKELFFSPSLHIQPRE
                     LLLVFCEVIENFNIKIPEEKLLNRQFTSFDNVLKIINMV"
     gene            4695..4898
                     /locus_tag="PC545_003520"
     CDS             4695..4898
                     /locus_tag="PC545_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458233.1"
                     /translation="MSFGKRIEMNNIGVETRGVCTIMCPYTCKMMASSSCNCKNNDYN
                     ARGGSYNTYYNKLIHGQRTPDGM"
BASE COUNT         2098 a          455 c          827 g         1781 t
ORIGIN      
        1 tgttatatta tattaattaa taaaatattt tatttcaatt ttattaagta tggaggtgta
       61 ttgtggaaaa tggtaaattt caatttttta aaggtgtaag aaattgtttt aatatgattt
      121 atctacatga taaaagatat ttatttatct ctatgtttat ggcaattttt caggggattg
      181 ttccatctat ttctttgtta gtaagtcaaa atatcattaa taatatgcaa acattttcga
      241 aaagtgtaaa agaaatatta gttttgttaa ttatgtattt aatcataaat ataattcctt
      301 caatttttca aagtttttat ggatattata gaataaagtt taataaaaat tttgaaaaac
      361 atattgaaac aatgatgtta gagaaatctt taactttgag tttaaaagat tttgaggatg
      421 ataatactta tgacatgata aatagagctc aatctcaaaa aggatttaat attactatac
      481 tatctgaaaa ccttatttca ataataagag aatcagtatc tacactttct cttattgttg
      541 tattatcaca atttcaactt tggctaataa tagttgttat tgttattcct gtattgaaat
      601 cattatattc cattaaatta ggaaaggaac aatatgatgt tagtgtaaac agaacttgga
      661 aagaaagaaa ggcctggtac atagattatt tgttgtcaaa aggttatgct tacaaagaga
      721 ttatggtatt tagaattggt tctaaactta ttaatcatta taaaaaatta aaagaggata
      781 ttatatctca agatatgaat atagctaaaa agaattttat tgctacgcta ttatgtacag
      841 ttttagattc aattgtatct gcaggaatta taggatttat aataattcaa ggtattttaa
      901 agaagatttt aataggagat gcgactacat atatacaagc agttcaatct ataaaggata
      961 atgttgaatc aatattttcg ttatttagtt cagtaataca agatcttttt tatacagatt
     1021 tactgtttaa atttctagat tataactgta aagaagactc aattgaacat ataaaaatat
     1081 ttgaaaaaat aggggatata aataaaatag agttaataaa tatttcatat aaatacaatc
     1141 attcatcaga gtattcttta aaaaatatta atttaacttt aaataaaaat gatcccattg
     1201 ctatactagg aaaaaatggt tcaggtaaaa cgacactttt aaaaatatta ttaggttttt
     1261 atgatgatta tgaaggtgac atcttgataa atggaaaaaa tttaaaggat attgaaaaag
     1321 atgaatacat tagaaaagta agttgtatgt ttcaagatta tattaaatat gaggctacat
     1381 taaaagaaaa tgtcgccatg ggagatgtat ataagagtaa cttggaagaa aaagttaaaa
     1441 gtaaacttaa agaaactaaa ttgaaaccaa gtatttatga ggaagatgga ttagaaacta
     1501 ttttaggaac ctggtttggt aagaagcaac tttctatggg tgaatggcaa aaagtagcaa
     1561 tatctagaac tcttatgaaa gatgctgata tgtacatttt tgatgaacca gattcatctt
     1621 tagattctga agcgggaaat gaactccttt ctttatataa aaaaacattt gaaaataaga
     1681 ttggaatatt tataacgcac catatccaac atgcaaaaaa gataactaac aatattgtag
     1741 ttcttgaaaa tggaaagttg ttagaaagtg gaagacatga tgaacttatg gacaaaaagg
     1801 gtgtttattt taatttattt tgtaactgca aataaatatt aaagggaggt taattaatga
     1861 atcaaaaacc atttatacac acatttaaag ctggaaaatc atattttatt tatgatgtta
     1921 atacggataa gattttaaag gtaaatgctg ctgtttataa ttatctaaat aaaattgagc
     1981 ataatttaga aaaagaaagt gactggaaca tagaaataga agacgaaatt aataccttga
     2041 ttaattatgg atttctaaaa aataaaaggg taagtaaaac aatgcaccct gaaactaagt
     2101 atataaaata tcatatagaa aataatctat cctctattat tttacaagta acccaaaatt
     2161 gtaatttacg ttgtgactat tgcatttatt ctggttcata cagcaacaga gttcatacaa
     2221 ataaaagaat gaatatagat gtagcaaaaa gaagtattga ctatttgatt aagaattcta
     2281 gtcaattaaa tgaagttagt gttggctttt atggaggaga acccttactt gagtttgatt
     2341 tgattaagca gatagtagaa tatgtaaaag tagttggaga aggaaaggaa atatacttta
     2401 atctaactac aaatgcaact ttatttaatg aggaaataat agaattcttt gtaaaaaata
     2461 atataacaac tatgattagt ttagatggag gaaaagaaat tcatgataaa agtagaaaat
     2521 ttgcaaatac aggagaagga tctcatgatg ttgtattaaa aaatgtacgt tacataaaaa
     2581 ataaatatcc agaatattat aaaaagaata ttacatttaa tacagtgata aattcagaaa
     2641 atgattacat ttctatagat gattttatat ctaaaaatga gactttaaaa gattcaaact
     2701 ttttagcttc cactcttaat aataactatt ctgataaaga aatagtttca tcagatgaat
     2761 atatattagg taagagttat gatagattct tgactttact caacaaatta aaatttataa
     2821 aagatgagag tatgtcacct ttatcaagaa tagaaagagc cttattagat gattttagat
     2881 atgagaaaaa tattgaaggt agattagagt taccagatgt gtggcatcat ggtggaccat
     2941 gtataccagg agcttttaga atatttgtaa ctacagatgg agaatttttt ccatgtgaga
     3001 gagtaagtga aagttctaaa atgggtatga ttggaaattt agatagtgga gtagatatag
     3061 aaaaggctga aaaactttta aatataggaa aattatatga agagcaatgt tctgattgtt
     3121 gggcctatag ttattgtgat atatgtatag ctaagattga tgatactaaa tgtaatgaaa
     3181 atgaaataaa accacatgat tgtgacagaa ttagacattc tacagaaaat aatatgaaag
     3241 attactgcgt tttgaaatct ctaggatatc aatattaaat tttctaatga aaggaaagat
     3301 gggattatgg aaaaagtact tatatatcca tttaattata atttttttac tattttaaat
     3361 cattgcaata aaatttcaga tatagaaatt aattcactgg ttagtccttc tggctggggg
     3421 ttatgcaata aggagtataa tgttaataat attattacta aagttacaaa tgattttcaa
     3481 agtgaactta aaaagaattc tttggtatgg tttgttgatt caaaagaaaa tctagactta
     3541 gaaaaattta taattcctaa attaaaagta gctctaaaag aaggtaaaaa gatattatat
     3601 acacgtgatg atcttaataa aatatctagg tatatatctc attatgaatt caaaaagatt
     3661 aatgaatgga atttagaaaa atatgaaata agagatatag agggtatatc acagataaac
     3721 acaccaataa tatttatttg tggtctttat ggagggttag atacttttga tgtaaatata
     3781 aggataggag aagaactaag aaaaaggggg ttttcaatat tacaatttgg ccctaaaaaa
     3841 gagagctatt tatttaaaat gattcctata ccagagttta tgtttgatga ttcgataagt
     3901 agtaagacaa agattattaa ttttaaccat tttataaaag ataaagaggt agaggcaaag
     3961 ccagatttaa taattataga agttccagga gaattattcc ctttctctag aaaaatggtt
     4021 ggtaattttg gaattagagc ttttgaaatt tctaatgcag taataaatga ttgtggtata
     4081 ttatgtattc catcaagtat atatgataga gaatattgtg aagaacttaa atattttatt
     4141 aaaggaagac atggtataaa tatagactat tttagtatag agaaaaaagt tatagataca
     4201 tatgagacag aggtgaagca aaaattttca tatatgtcat tagatgaaaa cttttttgtt
     4261 gaagatatag aagattatga aaaaacattt tttatacatg ataataaaca attactagaa
     4321 ttagtggaca atatagtgaa tcaattaaaa gagtatgcta aagtaatatc tatttagaat
     4381 tggaggttaa ttatgaatta cactgaaaag gaattactac tagagaatat attttttgat
     4441 gtgactggaa ttaattttaa taaaaataac aatttaaaaa aagaattatt tttttcaccg
     4501 tctttacata tacagccaag agaattactt ttagtatttt gcgaggtaat agaaaacttt
     4561 aatataaaaa ttccagagga aaaacttttg aataggcaat ttacgagttt tgataatgtt
     4621 ctaaaaataa tcaatatggt ataggttaat aaaggtataa agtaagtgat cttatagaaa
     4681 aggggaggat atgaatgagt tttggaaaaa gaatagaaat gaataatata ggtgttgaga
     4741 caagaggtgt ttgtactatt atgtgtcctt atacttgtaa gatgatggca tcttcatctt
     4801 gtaactgcaa gaataatgat tataatgcaa gaggaggatc atataataca tattataata
     4861 aattgataca tggccaaaga actcctgatg gaatgtaaat atttcagtaa taagtatact
     4921 attaaattga tttagcatat ttgattaaat ttatctttct tactttattt ccttttatta
     4981 taaaaatatg cagttgatta taattaagag tgtgatttta taataactgc ataaatagta
     5041 tctgttatat aaatcacctc ttcaattatt atagtggtaa atatagtgat taggggtgat
     5101 ttttacaatt taaaagtata aattaattaa aagttattat aataaattat acttatttaa
     5161 a
//
LOCUS       DAMJVI010000171         5026 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.176, whole genome shotgun sequence.
ACCESSION   DAMJVI010000171 DAMJVI010000000
VERSION     DAMJVI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.176"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..324
                     /locus_tag="PC545_003521"
     CDS             <1..324
                     /locus_tag="PC545_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2458234.1"
                     /translation="ICIEDLQVKNMIKNHKLSRLISDVSWSEFIRQLEYKANWYGRQI
                     VKVGKFFASSQICNKCGYKNEEVKNLNIREWICPSCNETHDRDINASINILKEGLRLI
                     TIQNK"
     gene            451..1620
                     /locus_tag="PC545_003522"
     CDS             451..1620
                     /locus_tag="PC545_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDF2458235.1"
                     /translation="MNELCKDEVVEFCNRIKGKVESVAISGVFAPVNKQQEEEVAKWV
                     REILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALKDLGIDAQIY
                     FSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGGTTSDLGVLY
                     NGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPESVGYRITEDA
                     IIFGGDTLTLSDVVTKLDMAFEDRKSNVENLDKEYCNEVYKKVILKLEDAVDQMKTSQ
                     DDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVEKVYSLDKMS
                     REDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVKVVGDLI"
     gene            complement(1783..2325)
                     /locus_tag="PC545_003523"
     CDS             complement(1783..2325)
                     /locus_tag="PC545_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HDF2458236.1"
                     /translation="MKSYIALLRGINISGKNKIIMSELKASFVELGYSEVSTYLNSGN
                     VVFLSDVDDSKVISNTIRLMIKKQFELEIPVFIISQEELKDILNNAPNWWGDNNKEIY
                     DNLIFMFPDLSYDEFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYKKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(2473..2757)
                     /locus_tag="PC545_003524"
     CDS             complement(2473..2757)
                     /locus_tag="PC545_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2458237.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYIL
                     VYRENEDKEKNEHDKNLNETINEFKKEEEKRMQAKSAARAIAEAKKRVLY"
     gene            complement(2820..3584)
                     /locus_tag="PC545_003525"
     CDS             complement(2820..3584)
                     /locus_tag="PC545_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDF2458238.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAQASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMINTIENFRKSNKTFKEF"
     gene            complement(3866..4132)
                     /locus_tag="PC545_003526"
     CDS             complement(3866..4132)
                     /locus_tag="PC545_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HDF2458239.1"
                     /translation="MIESRCGILCYECGYKEKVNCKGCTEIEKPFWGEQCPVKSCCED
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRISQCKKWAVLAL"
     gene            complement(4174..4983)
                     /locus_tag="PC545_003527"
     CDS             complement(4174..4983)
                     /locus_tag="PC545_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458240.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLIPDASGCMIFKFDKKGIESAFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIEELVEQLDMLFLSYLLKSNIVDMT
                     IPILENARKQNSIMSVKNISQISCYSERHLNRIFNNSLGMSVKSYLRLLRINLVLQEI
                     QNNKIPFATLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
BASE COUNT         1740 a          687 c          715 g         1884 t
ORIGIN      
        1 atttgcatag aagatttaca agtaaagaat atgattaaaa atcataaact atctcgctta
       61 atttcagatg tatcatggtc agaatttatt cgtcaattag aatacaaggc taattggtat
      121 ggtagacaaa ttgtaaaggt aggtaaattc tttgcaagtt cacaaatatg caataaatgt
      181 gggtataaaa acgaggaagt taagaactta aatataagag aatggatttg tcctagttgt
      241 aatgaaactc acgatagaga tataaatgca agtataaata tattaaaaga aggattaaga
      301 ctaataacaa ttcaaaataa ataacgtatg taagaaccgg aggaactacg gggatagcct
      361 gtggagaatt agtaagacat attttagtat gcaaaattct gtgaagcagg aaccctgtga
      421 ctttagtcat gggaggttca gggtagaaag ataaatgaac tttgtaaaga tgaagttgta
      481 gagttttgca atagaataaa aggtaaagtg gaaagtgttg caataagtgg agtatttgct
      541 ccagtaaata aacaacaaga agaagaagtt gcaaaatggg ttagagaaat tttaggggat
      601 attcctatat ctttatcaaa tgaaataggt aatataggta tattagaaag agaaaatgga
      661 gctattttaa attctgcatt gtctcaagta ggtaaagctg tatctttagg atttgaaaaa
      721 gcacttaaag acttaggtat agatgctcaa atatatttca gtcaaaatga tggaacatta
      781 atgaatttag agtatacaat gaaataccca atttttacaa ttggatgtgg aataacaaat
      841 tctataagag gagcttcctt cttatcaaaa gtaaaaaatg caatagtatt agatgttggt
      901 ggaactacaa gcgacttggg tgtactatat aatggattcc ctagagaatc atctattcca
      961 gttacaattg gaggagtaca tacaaacttt agaatgccag atgttttaac tattggtctt
     1021 gctggtggta catgtgtaaa aggcgaaaaa gataatataa agataggtcc tgaaagtgtg
     1081 ggatatagaa taactgaaga tgcaataata tttggtggag acactctaac tttatcagat
     1141 gttgttacaa aattagatat ggcgtttgaa gatagaaaaa gcaatgttga aaacttagat
     1201 aaagagtatt gtaatgaggt atataagaag gttattttaa aattagaaga tgctgtagac
     1261 caaatgaaga ctagtcaaga tgatgttgaa ttggttttaa ctggtggagg aagtataatt
     1321 gttcctgaaa aactaaaagg cataagcaat gttataagac ctcctcatta tactgctgca
     1381 aatgctattg gtgcagcttt aggacaaata agtggagatg tagaaaaagt atacagttta
     1441 gataaaatgt cgagagaaga tgccatagaa gatgcaaaaa aagaggctgt aaataaagct
     1501 ctaaaagctg gtgctaaaga agatactata gaagtattaa atatagaaga tgttccactt
     1561 gcataccttc ctggtaatgc acttcttata aaagtaaaag ttgttggtga tttaatttaa
     1621 agaataaata taagtatttc cagaattata taaatatttc tggactcatt tttaaactta
     1681 ttttatcaat aaaaacagca taacttttga ctatattctt ataagtttcc gaacagccga
     1741 tatataccta ttttttgagg tttatcggct gtttgttatc ttttatcgca ttccaactat
     1801 ttttcttacc gtatttgctg tacgaattgt aatcttatca cttacatttg aactggcagt
     1861 ctttgaccac caatttgtct ttttataatc tttacgacta aaagaccaaa agatagcatt
     1921 tttataatga tatacttttt catactcttc tttaggattt ccaacctcat cataaaactc
     1981 gtcataagat aagtcaggaa acataaaaat aagattatca tatatttctt tgttgttatc
     2041 tccccaccaa ttaggagcat tatttagtat atcttttaac tcttcttgtg aaataataaa
     2101 aactggtatt tccaattcaa actgtttttt tatcattaat cttattgtat ttgagataac
     2161 cttgctgtca tctacatcac ttaaaaagac aacattacca ctattaaggt atgtagaaac
     2221 ttctgaataa ccaagttcta caaagcttgc ttttaattca gacataatta tcttgttctt
     2281 accacttata ttgatacctc ttaataatgc gatataactt ttcatataat aatttcacct
     2341 ctaaacattc acatttttct atgtagtaca aactggaatt tgcatctttt ataattcttt
     2401 caaaacttct tcatatgtaa tttttttaat ataaaaaaat cggtgcttaa tttgtaccga
     2461 tttttgtttt tcttagtaaa gaactcgttt ttttgcttct gctatagctc tagcagcact
     2521 ttttgcttgc attctttttt cttcttcttt tttaaattca tttattgttt catttagatt
     2581 cttatcatgt tcattttttt ctttatcttc gttttctctg tatactaata tataatctat
     2641 tattgattcg tttattattt gagatatact tttattttca ttaattccta ttctcttaac
     2701 ttccttaagt agagtctcat caatatgaac cgttgtttgc tttgtatttt ttgccacctt
     2761 aatcacctct taaaaaaaat tctaacatta aaattttttt tagtcaataa attttaatat
     2821 taaaattctt taaatgtttt atttgatttt ctaaaatttt ctattgtatt tatcatctta
     2881 ttttttatat tttcacctag tacagaagac tttagtctag gattttcgat atggtcataa
     2941 attggttcta aaaattcgac ttcgacagga acagagttaa ttttaccaat aggttggtat
     3001 ccttcataag tatcttttga attttttatt actagtggaa caatagggca ttttgcttta
     3061 tatgctatct ttaaagctcc acttctaaac tccgaaacca atgaatttgg ttctttaatc
     3121 catgttaaat ctccttctgg aaacactgcc atactttgac ctgtaagtat attttgagag
     3181 gcttgggcta tactctttat accctcacga ttgttttttc tgtttacata tacacatctt
     3241 agtaattttg cccattcttt aaatataggt atatttctcc aaactggttc atctgcaatt
     3301 acacatccaa ttggtctttc tacacttgct gttagtatga agctgtcaag catactagaa
     3361 tgatttacaa caaatagaag tggttctttt ggcagtgttt ccttaccaat tatattaagc
     3421 tctatgttca ctatatctaa agatttttta gcttgttttt gcataaattc aaatttttcc
     3481 tttaagctaa atttatcagg attcttttta attttaataa gcctaggtat agaagatgta
     3541 aaacctataa gttgatatgt agcaaatttt aagtaattat tcataaaatt atctccttaa
     3601 aataaaatta tttattactg taaagatttt aacataaaat aaaatttaag tctattttaa
     3661 ttcaattttg taacatatat tttaaataga cagagattga gattttatat ttccaatttt
     3721 agtaatcaga ttttatcaat aaattactat tctattaatt taattaaata acctttaaat
     3781 ttaattataa aaactgttgc agaaatttct acaacagttt aaaaaattta ccttacaaaa
     3841 acttatttta taacaagaga gtatattata aggctagtac agcccatttt ttgcactgag
     3901 aaattctttt gccatcatca ccttgttctt tgtcatatgc aaattgatta agcatttcac
     3961 atggaaatgt atcacataga ccacaatgtg taaggttttt atcttcacaa caagatttta
     4021 caggacattg ttcaccccaa aatggtttct caatctctgt acaaccttta caattgactt
     4081 tttccttgta tccacattca taacataaaa ttccacatct tgattctatc attttaagtc
     4141 actccttaaa tataaatttt atatgattgt atatcaaaat ttatactttt cattgtaaaa
     4201 atcggacaaa ttttttatat aggttgttgg attaacacca caaatagatt taaaatcatt
     4261 tataaaatga gattggtcgt aataaccaat atcttgtgct aaagttgcaa atggtatttt
     4321 attattttgt atttcttgta atacaaggtt tatacgaagt aatctaaggt atgatttaac
     4381 actcatacca agagaattat taaaaattct atttaaatgt cgttcactat aacaactaat
     4441 ttgtgaaatg tttttaacag acataataga gttttgtttt ctagcatttt caagaattgg
     4501 tatagtcata tctactatat ttgatttcaa taagtatgat aagaataaca tatctaattg
     4561 ttctactaat tcttctatag tacttgttct ttcaaaaatg gaattaacct ctaagctaaa
     4621 taatgtgtta aaatcttcaa gtgaaatttt taaatctgta gattctcttt gagaaagacc
     4681 tgtcaaataa tatactccac ctgggcgaaa ctcaacgaat actctaaaaa gtacattttc
     4741 tatatcattt tttacaatag tagtttttgt tgtggctccc cagaatgcgc tttctatacc
     4801 ttttttgtca aatttaaata tcatacatcc actagcatca ggtattaatg taagattttc
     4861 tttaatactt gtatctggaa tagatattgt atagtgtgct atatactgtc ttaacaattt
     4921 atgtggcaaa atatatgtat actgtttgca ggtatttaaa atgttattga ttattttatt
     4981 cataagtata tcctcctgat atataaaaaa taagtatact aattta
//
LOCUS       DAMJVI010000172         4976 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.177, whole genome shotgun sequence.
ACCESSION   DAMJVI010000172 DAMJVI010000000
VERSION     DAMJVI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.177"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(547..1188)
                     /locus_tag="PC545_003528"
     CDS             complement(547..1188)
                     /locus_tag="PC545_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDF2458241.1"
                     /translation="MNNNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKEISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDIGILTGNITTAAQALGLGSCIIGMVRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYRELLDIPEGYSVPFGIAVGIPDGNLPNAREVVYKVSRV"
     gene            complement(1431..2234)
                     /locus_tag="PC545_003529"
     CDS             complement(1431..2234)
                     /locus_tag="PC545_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDF2458242.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEESSKGKNEVMKEVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGIESIAKVYVTCTKHPDGVEMARDIHQSMKDLIASFSQDPSIIPGASKGNDDFRKY
                     LLDNKYKNGWPTIPDEFKAKYGLSKDTLYIQPYAYSPTKSDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            complement(2390..3565)
                     /gene="thiI"
                     /locus_tag="PC545_003530"
     CDS             complement(2390..3565)
                     /gene="thiI"
                     /locus_tag="PC545_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDF2458243.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGRFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIEA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDRINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSILPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            complement(3611..4762)
                     /locus_tag="PC545_003531"
     CDS             complement(3611..4762)
                     /locus_tag="PC545_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDF2458244.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     AIKEVRQDIAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKNAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRLSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDVVISKVNNLKNILKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSYV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
BASE COUNT         1562 a          938 c          469 g         2007 t
ORIGIN      
        1 attgaaaatt tagatatttt tctaaaaatt atctatgtta atagttaaat ttatgaacat
       61 tttttgtttt tatgttagga tatttcttag aaatgaaata cttcttaatt tgatttgata
      121 tgtatatttt tatagctatt ttattataaa aaaaaagaac ccttttataa ctaagggtta
      181 ctttttttct ctattttttc ttattatatc actatttttt ccctatttca atattttgac
      241 attttttcca ttatactgaa aagatatctt aaatttttaa cttggataaa ttttaaagca
      301 tatacttaaa attaacatac ttgttttcta ttttattaca cttaatcctt atttttatca
      361 ttctacaaaa caaaaataaa aaagaataag ataataaata aaatacttat caccttattc
      421 tttatttaat tattttacaa cgaaaaatct aaactacttt ttaaatagct tcctcttcca
      481 tcaatttatt tgtatattac tggtttaatt cttcaagtaa ctgattggtt cttgcaatac
      541 tttatcctaa actctggaaa ctttataaac tacctctcta gcatttggta aatttccatc
      601 tggaatacct acagcaatac caaatggtac agaataccct tctggtatat caagtagttc
      661 tctgtatttt ttatttgtat caaacttcat ctttattgac tctgattctt ttacatcttc
      721 tttatccaat acactcacaa gaccttcaat atccccttgt tcaacaggtg taaataaacc
      781 tctaaccatt cctattatac aacttccaag ccctagtgct tgtgcagctg ttgttatatt
      841 ccctgtaagt attcctatat catatggagc ccatgaacta ctttcatcat aagaaataaa
      901 taaaacggtt ggagcaccat aaaataactt aaagttttta tttatctcaa cattactcac
      961 ctcatgaatc ctagataaag tatcatcact aatctctttt aaaagtttct ggtcttgcac
     1021 aacagttatg tgccaaggct gtttattaca tcctgaaggt gctgcaaatg cacagtgtaa
     1081 taatgtgttt acttgctcat cactaatttg ttctgtcgta aattttcgta tacttctacg
     1141 actttgtatt aaatttattg tttggttgtc ttgaaaatta ttattcatat ctattcccct
     1201 ttttatgtat ttcttagtac aaatactaaa tcttcagaaa attaatctta taattaaatt
     1261 tctttacaat attattataa ttatttttag cttgtatgtg aagacttaaa aatataaaca
     1321 cttttaatat atctcaatta tatatataaa tgaaaatctg gtattcgtca tctatagaaa
     1381 ctgctctttt ctatagttta atcaatacca gattaataaa acatgatata ttaatttaaa
     1441 ggaccccatc cctctttaga atgaatctct gtttttacag acttaacact atctgtgtca
     1501 acatcccatg acctacctgc aacagaaata ccatttttac ctttatacca tctaccttca
     1561 tcataatcgt aaactaggga agtataccaa ttacccccag tcttattatt tgcaaataca
     1621 actacagtag catcagattt agtaggacta tatgcatatg gttgtatata tagtgtatcc
     1681 ttacttaatc catattttgc cttaaattca tctggaattg taggccaccc atttttatat
     1741 ttattgtcta ataaatattt tctaaaatca tcattaccct ttgaggctcc tggtattata
     1801 gaagggtctt gtgaaaatga tgcaatcaaa tctttcatac tttgatgtat atccctagcc
     1861 atttcaacac catctggatg ttttgtacaa gtaacataca cttttgcaat gctttctatt
     1921 ccagttattc catcataacc atcatcaaac gttgctgaat atattccacc tgacttacac
     1981 tttggttctg tttcaaagta cttaccatct ttgttttcta atacctcttt catgacttca
     2041 ttcttgcctt tacttgattc ctcagccatt gcaataacaa tttgagtctt tatattttct
     2101 ctattagaaa gacatgtaac tgctttactt ttttctatat ttctaaacaa tgctggaaca
     2161 gctactataa ctaaaatgcc tattatagat ataactacta acaattcaat tagtgtaaaa
     2221 ccctttttat tcataataat cattcccatt aaataaaatt atagtactta tttttaaata
     2281 ctacatatta ataaattgtc tacataaatc ctttcaacag aactactaat atacttacag
     2341 aactactaat atactcaata ttcaatttag taattaaata acttgtatgc taaaactcaa
     2401 tatcctctac ctctattttg tcaatagcat cctgtactaa tttttcaatg tcaagtgctg
     2461 tttcagattt ttctattttc tctaatctag gtttagtgac gggctttcta ggagaaaata
     2521 cactacaaca atcttcttca ggcaatatag atgtttcaaa agtccctatt tttttggcta
     2581 tatcaactat atctgattta tccattgcta taagtggtct aaacactggt aagtctacaa
     2641 cggcattagt acaagtaagt ccttgtatag tttgagatgc aacctgccca atactttcac
     2701 ctgtaattaa agcatcacaa tgtctttttt cagaaagtct ctgtgctatc ctcatcataa
     2761 atcttcttga aagaatagta gcttcttctt cattacaatt ctcgccaatt gctttttgta
     2821 tttctaagat attaacttta tgaagcctta ctctaccaca gtattttgcc agtattttag
     2881 ctaaatcctt aactttttcc tgtgaccttt cacttgtaaa tggataacta tgaaagtgaa
     2941 ctgcctctac ttccatacct ctttttgcaa ccatccatgt agctactggg ctatctatac
     3001 ctcctgataa aagagacata gctctaccat tcgttccaag tggcaatcca ccatatccag
     3061 gtatagtatc actatagacc atagtatgaa attctctgta ttcgcacttt attttaactt
     3121 ctggatttct tacatcaaca ttaatcctat ctcctacctt tgataataag tatccaccta
     3181 tatccatact catttcttga gaagtcaacc cgaaagactt atctcctctt cttgactcta
     3241 ccttaaaagt tttatatcca gcctctattt tttcttctaa catttttaaa gctatttctt
     3301 ttaatgtatc ataatctttt ttagctctta ctccagggca cacacccact atcccaaata
     3361 cttttctaac ttcttcaatt acttcttcat aatcatagtc ttctaaatca acatatattc
     3421 ttccatactc tttatataca ttaaatcttc caattggttt taacatattt cttatgtttc
     3481 ttataagcct attctcaaat atatatctat tctttccttt tactccaatt tctccatact
     3541 ttactattaa tatattatac accatttcac ctcttaaact aatttattat cactatctat
     3601 acagtattct ttattttctt ttcattattg accttaagtc acaaattgat tctttcaaaa
     3661 ctttcacagc ttctaacatt tcttcttctg tattcatatc agataaacta aatcttattg
     3721 ttccatttat ttcttcattt gtaagaccta tagcatttaa tacgtaacta cctttctttt
     3781 tagaagaaca tgcagagcca gttgatacat aaataccctt ttgctctaag taatgaagta
     3841 aaacttctcc ttttgtccct ctaaatgata catttaatat atgacatact ccatcttctg
     3901 gagaattaat ttttatatct tctatattat caattatttc attttttaat atatttttta
     3961 agttatttac tttcgaaata acaacatcta aatctttgtt tagtattcta acagcttctc
     4021 caagaccata tattccaggt acattttctg ttcccgacct aattccaatt tcctgaccac
     4081 cacctgttaa cataggctta aggcgattat tttcttttat atacataaat ccaattccct
     4141 ttggaccatg aaatttatgt gcacttacac tcataaaatc tatattgtat ttagatagtc
     4201 taaaattaat ttttccatat gactgaatag catcaacatg gaaataaatt ttttctttta
     4261 aacccttcaa atactttcca acttcaccta ttggctgtat agttcctatt tcattgttaa
     4321 catgcatcat gcttactaag catgtagttg actttatcgc attttttaaa tcctctatat
     4381 ttattttccc atcttttccg acctctaaat aagttacttc aaaaccatct tcttctaaat
     4441 cttttaatgt atttaaaaca gaaggatgtt ctatgttagt agatattatg tgattttttc
     4501 tttttttatt taaactagta atacctctta ttatagtatt attacactct gtaccacctg
     4561 atgtaaaata aatctccttt tctttcgctc ctagagattt agctatatct tgtcttactt
     4621 cttttatagc tttttcaact tctacacctt ttctatggac agaagaagga tttccatatt
     4681 ccgtattaag tgcataaacc attttatcta taacttcttg ataaggtttt gtagttgcgc
     4741 tattgtctaa gtatatttcc aacttaatta cacctacctt tatttaaaat cttcaactct
     4801 aactattata ccactaatta tagtattttt tttgttaatc tttattaaaa agacaatctt
     4861 aaattataaa atattatttc atgaaaagct agttttttca ctttattaca aatttttata
     4921 gtaaaatata atatacttat actataccct acaaaaatat attataaaat acaata
//
LOCUS       DAMJVI010000173         4750 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.179, whole genome shotgun sequence.
ACCESSION   DAMJVI010000173 DAMJVI010000000
VERSION     DAMJVI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.179"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..664
                     /locus_tag="PC545_003532"
     CDS             <1..664
                     /locus_tag="PC545_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008819588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HDF2458245.1"
                     /translation="ATGPTGATGATGADGATGPTGATGATGVTGATGPTGPTGATGAT
                     GATGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDLTNAAGTL
                     TNFAFSMPRDGTITSISAYFSTTAALSLVGSTITITATLYQSTAPNNSFTAVPGATVT
                     LAPPLTGILSVGSISSGIVTGLNIAATAQTRFLLVFTATASGLSLVNTVAGYASAGIA
                     IN"
     gene            833..1594
                     /locus_tag="PC545_003533"
     CDS             833..1594
                     /locus_tag="PC545_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HDF2458246.1"
                     /translation="MKNKIEISMNNFVKNYHKSNNITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLENGKTIITYFIPFDESVNNSNIGGFECSEIWARAYIETNMLIS
                     NLNDFLCEELEKIGANSYAIPGSYNFDSEKLVSDWSQRHVAFIAGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDGFNRNKCY
                     EMCMINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            1903..2181
                     /locus_tag="PC545_003534"
     CDS             1903..2181
                     /locus_tag="PC545_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458247.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGECIYIPRKSNNKKEWGSNTP
                     TREELSIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            2474..3049
                     /locus_tag="PC545_003535"
     CDS             2474..3049
                     /locus_tag="PC545_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HDF2458248.1"
                     /translation="MKKITWEVEYISQLPVIRYCKKCGKKTEYICSGLFRVNAQHKYL
                     DIWLIYKCSNCDSTWNSTIYSRVNPKSLNPEILEQFHTNDYNLVKQYAMNMELLHRNG
                     AEVGIPEYKIVGQEININDLTQVHIISKYPCQLKVSTLLREKLGLSRKVFDEMLDCGI
                     IRSNSGVDLKKGKLNYEIMLDIGKEKQKSSN"
     gene            complement(3155..3988)
                     /locus_tag="PC545_003536"
     CDS             complement(3155..3988)
                     /locus_tag="PC545_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HDF2458249.1"
                     /translation="MNVENQSLSDIEIILSHRYDNGADYWTTPDKRLIKGSPFSTLDS
                     VLYLLELGVNPDDTLLKECADLIFSTWREDGRFKLYPNGGIYPCHTANAANVLCHMGY
                     ASDIRMQKTFQHFLDTQYTDGGWRCNKFSFGRGPETEYSNPLPTLNILNAFRFSNYLN
                     KESKLDKAVDFLLDHWTIKKPIGPCHYGIGTLFMQVEYPFRNYNLFLYVYVLSFYNRA
                     KKDKRFLEALKILESKMIGNKIVVERVVPKLSKLSFCKKGESSEIATTHYYEILKNLE
                     K"
     gene            complement(4056..4685)
                     /locus_tag="PC545_003537"
     CDS             complement(4056..4685)
                     /locus_tag="PC545_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HDF2458250.1"
                     /translation="MDEKITKNNYNVPFDYVRKKLKELDPHTISNLSNIPFNNDLNRF
                     TLNFFDRQYIIDYPSGEVWNSNGSLCNNFELKILIVRYLINAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLYRLSEVFTSQIDKYKLVMEKIGAEKVNMGDLAYRFTYMGNTSMV
                     FILWFGDDEFPPNSNILFDSNIVYYFNAEDLAVVPDTAINAILNKINQL"
BASE COUNT         1752 a          617 c          775 g         1606 t
ORIGIN      
        1 agcaacaggt ccaacaggag caacaggagc aacaggggca gatggagcaa caggtccaac
       61 aggagcaaca ggggcaacag gagttacagg agcaacaggc ccaacaggcc caacaggagc
      121 aacaggagca acaggagcaa caggtgctag tgcaataata ccttttgcat caggtatacc
      181 actatcactt acaactatag ctggaggatt agtaggtaca cctggatttg ttggatttgg
      241 tagttcggct ccaggattaa gtatagttgg tggagtaata gaccttacaa acgcagcagg
      301 aacattgact aactttgcat tttcaatgcc aagagatgga acaataacat ctatttcagc
      361 atacttcagt acaacagcag cactttcact tgttggttca acaattacaa ttacagcaac
      421 actttaccaa tctactgcac caaataactc atttacagct gtaccaggag cgacagttac
      481 actagctcca ccacttacag gtatattatc agttggttca atttctagtg gaattgtaac
      541 aggattaaat atagcagcaa cagcacaaac tcgattctta ctagtattta ctgcaacagc
      601 ttcaggtctt tcattagtta atactgtagc aggatatgca agtgcaggaa ttgcaataaa
      661 ttagattaga taagaaaagg cttctaatat cgtattgata ttaggagctt ttttaagaat
      721 gattgatgaa catagtttta atattaatta gtgcgcatat tataaaatac tattatataa
      781 tataaaataa taacattaaa ttggaaatat agaattatct aggggtgata ctatgaaaaa
      841 taagattgaa atatcaatga acaactttgt aaaaaattat cataaatcaa ataatataac
      901 tacaaagtgg caagaacttt tgtttgctta tgcatctgct gaagatgata tgtttcagaa
      961 gctaaaaagt gttgtaggaa catctcatgc tatgccaaaa gattttctgg aaaatggaaa
     1021 aactataatt acatatttta taccttttga tgaaagtgta aataatagta atattggagg
     1081 gtttgaatgt tctgaaatat gggcaagagc gtacatagaa actaatatgt tgatttcaaa
     1141 tttaaatgat ttcttatgtg aagaactaga gaagataggg gctaattcat atgctattcc
     1201 tgggtcgtat aattttgaca gtgaaaaatt ggtaagtgac tggtcacaaa gacatgtagc
     1261 attcatagca ggtcttggaa cttttggttt aaataatatg cttattacag aaaaagggtg
     1321 ttgtggaaga ataggaagca taatcacaag tttagaatta gagccaacta aaagaccaga
     1381 aaatgaatac tgcttgtata aacataataa tagttgcaaa aaatgtgtca ataaatgcgt
     1441 gaaagaagct ttaaaagaag atggatttaa taggaataag tgttatgaaa tgtgtatgat
     1501 aaatgaaaaa aaatacactt atataggtgg atgtgatatt tgtggaaagt gtatagttgg
     1561 agttccttgt tcagtaataa acccatcaag ataatggatt taagttgact aataatttta
     1621 aagatagttt ataatgaaaa aattttagtt atcaaaataa atataatata aaaactaaat
     1681 aaatgtgttt aaaaaatgga taaataattt gtgttgattg acaaccagtt atactgttga
     1741 tagacttata tgtatcgctg tgatatataa ggagggttga gattttatgt ttgatttaat
     1801 ctttattttt gcatagcaag ggctattgca actatgaaat ttaatatata aatagccctt
     1861 tgctattcct aatgaaaaat tactatttaa taggagagca aaatgagcta taaaaaagca
     1921 atacatatat taccagaaga attacttgaa ttaatacagg aatatgtaga tggtgaatgt
     1981 atctatattc ctagaaaatc taataataaa aaagaatggg gaagtaatac acctactcgt
     2041 gaggagttgt caattagaaa tatgcagatt tacgaagact atcaagttgg atatgatttg
     2101 caatatttat cggaaaagta ttatttatct ttgaaaagta tccaaagaat tgtattacaa
     2161 gaaaaaagaa aagatatcta aggatgagcc agtatagttt tataattata ctggctcatt
     2221 ttcttttgat aaaagtgttt gaggtatagt ttcttttaag tggatgatat aatttaagta
     2281 atttcaataa cttatacagt agctaacaaa gctggttgta aagtatctaa taagaagtag
     2341 tagcttttta tatgtttgaa attaaaattt attaaaatta ggaggtcttg ttttgaaaaa
     2401 aatacttgat tatattccac cttttcataa ctttctatta atttagtaat cttatagaaa
     2461 ggaaattgca tttatgaaaa aaattacttg ggaggtggaa tatatctccc agttacctgt
     2521 aattaggtat tgtaaaaaat gtggaaaaaa gactgaatat atttgttcag gtttatttag
     2581 ggttaatgct caacataaat atttggatat atggttaata tacaagtgtt caaattgtga
     2641 ttcaacatgg aactcaacta tatattctcg tgttaatcca aaaagcttaa atccagaaat
     2701 actagaacaa ttccatacaa atgattataa tcttgttaag caatatgcta tgaatatgga
     2761 acttttacat agaaatggag cagaagttgg tattccagag tataaaatag taggacaaga
     2821 aattaatatt aatgacctta cacaagttca tattattagt aaatatccat gtcagttaaa
     2881 ggtatcaaca ttattacgtg agaaactagg tttatctagg aaggtatttg acgaaatgtt
     2941 ggattgtggg attattagaa gtaactcagg agttgattta aagaaaggta aattaaatta
     3001 tgaaattatg ttagatatag gcaaggagaa gcaaaagtct tcaaattgat tagaataact
     3061 aacttaatat attttcagga acatcattta aaaaaatagt gactttaaaa aatttaaagc
     3121 cactattttt taatatgttt tatctatata actattattt ttctaggttt tttaatattt
     3181 cgtaatagtg tgttgtggct atttcacttg actcgccttt tttacaaaaa gataatttag
     3241 atagttttgg aactacacgt tcaacgacaa ttttattacc tatcatttta gattcaagta
     3301 tcttcaatgc ttctaaaaat ctcttatcct tttttgcacg attataaaat gataaaacgt
     3361 agacataaag gaataggtta taattacgga aaggatattc aacttgcata aataatgtgc
     3421 caattccata gtgacaaggg ccgattggtt ttttaatagt ccaatggtca agtagaaaat
     3481 ctacagcttt gtcaagtttt gattctttat ttagataatt actaaaacgg aatgcattta
     3541 atatattaag agttggtagt ggatttgagt attcagtttc tggacctcga ccaaaactaa
     3601 atttattgca acgccaccca ccatcagtat actgtgtatc taaaaaatgt tgaaacgttt
     3661 tttgcattct aatgtcagat gcatatccca tatgacagag tacgttagca gcatttgcag
     3721 tatgacaagg gtaaatacct ccatttggat atagtttgaa acgtccatct tctcgccatg
     3781 tgctaaatat caggtcagca cattctttaa gtaaagtatc atcagggttc acacctaatt
     3841 ctaatagata aagtacacta tcaagagtag agaaaggaga accttttatt aaacgcttat
     3901 caggtgtagt ccaatagtct gcaccattat catacctgtg tgataatatt atttcaatat
     3961 ctgataaaga ttgattttct acattcattt tttactccat ttcatattat attctattaa
     4021 atctatcaaa aactttatta taatattatt tatatttata attgatttat tttatttaaa
     4081 attgcattta tagctgtatc aggaacaact gctaaatctt ctgcattgaa gtaataaact
     4141 atattagaat caaataatat atttgaattt ggaggaaatt catcatctcc aaaccaaagt
     4201 ataaatacca tagatgtatt acccatatat gtaaacctat aagctaaatc tcccatgttt
     4261 actttttcag ctccaatttt ctccataact aacttatatt tgtctatttg agatgtaaaa
     4321 acttctgaaa gcctatatag agtcctgttt aaaaaatttg gataataaac attgcctcct
     4381 ggtatttctt taaaagttac atatttatta gttctaggaa tacctttagc attgattaaa
     4441 tatctaacta tcaagatttt aagctcaaag ttattgcaaa ggctaccatt actattccaa
     4501 acttctccac ttggatagtc tataatatat tgtctatcaa aaaaattgag tgtgaatcta
     4561 tttaaatcat tattgaaagg tatattactt aaattactaa ttgtatgagg gtctaattct
     4621 ttgagttttt ttcgaacata gtcaaatgga acattataat tattttttgt tatcttttca
     4681 tccataattt tttcctccaa tttaaacaat taaaagtata tttataaaaa gttttcttat
     4741 tattaatttt
//
LOCUS       DAMJVI010000174         4687 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.180, whole genome shotgun sequence.
ACCESSION   DAMJVI010000174 DAMJVI010000000
VERSION     DAMJVI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.180"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(131..934)
                     /gene="proC"
                     /locus_tag="PC545_003538"
     CDS             complement(131..934)
                     /gene="proC"
                     /locus_tag="PC545_003538"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDF2458251.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAEDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVTNSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKALFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(1259..3628)
                     /locus_tag="PC545_003539"
     CDS             complement(1259..3628)
                     /locus_tag="PC545_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDF2458252.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKQDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFQSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(3633..4541)
                     /locus_tag="PC545_003540"
     CDS             complement(3633..4541)
                     /locus_tag="PC545_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDF2458253.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSKCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIEKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKCADLFLYDIKLIDEDKHIKFTGKSNDLILKNVKILSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTKIGG"
BASE COUNT         1378 a          907 c          513 g         1889 t
ORIGIN      
        1 ctcaatcatt aaattccttt ttatagtaaa ttactcttat tttacttata ccaaatacat
       61 ttatgtatag atataaataa gcactaaaaa aacaattcaa gaaaccatat agtaaactta
      121 attacatctg ttatttactc atatctttag acttatctat acaagcaaca atagatttta
      181 taactgctgc tctaaagcct tcttcttcta ataccttaac agcttctata gtagttcctg
      241 ctggagaaca aaccatatct tttaattcac ctggatgttt tccagtttca agaaccatct
      301 tagctgaccc cattactgct tgagatgcaa atttataagc ttgttgtctt ggcattccag
      361 ctaatactgc tgcatctgcc atagcttcta taaacataaa tacatatgca ggagaagaac
      421 cactaatacc tgtaactgta tccattaaat attctgatac tatttctgcc tcaccaaaag
      481 attcaaacag agccttaatc atatttaact cttcatctgt tacattacta tttttacaaa
      541 gagctgacat tccttcgcca acaagggctg gagtattagg cattactctt actatttttt
      601 tatctgaacc tataaaactt tccagacttt ctatagtttt acctgctgct atagatacta
      661 ttattttatt tgcatctatt aattctttaa gtggagttaa agccttttcg catatattag
      721 gtttaagtgc tactattacc acatcagaat ttgtaacagc tacctttgaa tcagttgtag
      781 tatttactcc aaattcttct tttactttat ctaaggcaac ttggcttaaa tctgatacag
      841 ttatatcttc tgcctttact aatcctgact gtactatgcc acctatcatt gcacttccca
      901 tattccctga accaataaat cctaaagttt tcatatcaaa tctccttttt tctattagtt
      961 ttattttaaa gtagctaaat tgactttatt ttaactgctt aaaattatct tatttttata
     1021 aaatataacc ttattataac tataataaat tattcttttg tatgtattac ttactaatat
     1081 gtgtatgatt tactaaatat aaatttaacg attttttcat taaacttaac gagctaactt
     1141 cctatttaga aagttagctc ataattaatc taaaatttta aaaatggaat caaaccattt
     1201 atactttatt atatcagtta cgattttata tagtaagaag ttttatttac aagcaaacct
     1261 agaatgtttg ctcagttctt cctattatct catcttgtag tgttctactt aaattattga
     1321 aatggtcact atatcctgca actctaacta ttaagtcttt ataatcctga ggattctttt
     1381 gagcctctaa cagtacattt ttatcaaata cattaaattg tatatgatga ccatccatat
     1441 taaagtatgc tcttactaga ttagccatat tatctaaccc tttttctcct tgaactactg
     1501 atggtgcaaa tctttgattt aataatgttc caccagtttt tatgtggtcc atcttagcac
     1561 aagattttat aactgctgta ggtccattag tatctccacc tttttcaggt gaaattccct
     1621 ctgaaactgg ttttgcacaa agtctaccat ttggacttgc tcccataatt tctccaaagt
     1681 atatatgaca agttgttggt aacatatcta ctctgtattc tccaccacaa acagttggtc
     1741 taccagttac ctcattgtaa tatgcattga atatctcttg cattatttca tcagcataat
     1801 catcatcatt tccatattta ggagtcttat ttgaaactaa attaagtata gcttcatatc
     1861 cttcaaaatt agcatcaagt gctttaagca atgtatccat atcgaatttt ttcttatcaa
     1921 atacattata ttttattgct gttaaactat ctgttatagt tcctatacca acaccttgta
     1981 tatatctagt gttgtatctt gctccacctc tttggtaatc cttgcctttt tctatacaat
     2041 catcaactat tgtagacata agtggacaag gcatgtgttt tgcacatata ttttctatca
     2101 cagcatttcc ttctattttt atgtcaatca tataatgtaa ttgcttttta aatgcttcaa
     2161 atagttcctc ataagattgg aaatttctag ggtctccagt ttcaatacct atttgttttt
     2221 tactaattgg gtcatatcca ttatttaatg ttaattcaag tatttttgga atgttcatat
     2281 atccagttag aacatatgct tctttaccaa aacatccagt ttcaacacaa ccactggttc
     2341 caccaagtct tgcatcttct atagtttttc cagcttccat aagctcttga actattgctt
     2401 cagtattata aaatgctggt tgaccccatc cttttcttga tatttcacaa gctctcttta
     2461 agaatttttg aggagtcttt ttacttattt gaacatttga actaggttgt atcaatttca
     2521 tttcatccat aacatcaagt attatataac taacttcatt aacaccatct tgaccatctg
     2581 gatttattcc accagtgttt atatttgcaa aatctgtata tgtgctactt tcttttaatg
     2641 ttataccaac ttttggtggt gctggctgat tgttaaattt aacccataaa cattcaagta
     2701 attcttgtgc tctatctcta tctagtattc cattttctac atctctttca tagaaaggat
     2761 ttagatgttg gtcaagtctt cctggagtaa aagcatccca tatattaagt tcagtagtaa
     2821 cccctatatg aacaaaccaa tacatttgta tagcttgatg ataagtttct ggtttgtgag
     2881 ctggtacaac atcacaagtt tcagcaatta gtaataaatc ttgtttctta gcttcatctg
     2941 tttcttcttc tgccatttgt cttgctttct cagcatatct tttacctaat ataaccattg
     3001 catcacaagc tattgccata gcttctaaat ctgctttttt attatatgca tctaaatcat
     3061 taagaaaatc tagctctttt aatcttgctt ctatgtcttt ctttaaatct aaaaatcctt
     3121 ttttatatat tgtatctcca caaactgtat gtcctggggc tctttgttcc ataaactctg
     3181 taaacatacc tgcttcatat gctgctaacc actcaggtgt cattgcattt attatcttat
     3241 ctcttgtttg tctctttttc caaaaaggaa ttatttcttc tttatgtatt tttctagctt
     3301 cttctgatac gctaaaattt attatatctc tattgtgcat tacttctaaa tcttccattg
     3361 tatgacaaca aatttctgga tatgttggtg ctccattagg agaatcacct ttttcaccta
     3421 ctattaattc tccatcattt atgctaagag ttctattctc aatataatgc ttaaatgata
     3481 aagcacgaag tacaggaatc tctacacttc cctcatattt tttataagct tctgtcataa
     3541 gtactgctct ttcaatagat atgtgtggtt ctgcattaat actttcttct cttaattttt
     3601 tagttctctc aaaagttcct cttgccattt tattaccctc caatcttagt attgaaatta
     3661 cttgcttcaa acagtctttt tatttcctct aatttttctt ctgatggtct ttgtagttct
     3721 tctccttcat actttttata aattttatca tacttgtgtt taccaatatt atgatatgga
     3781 agaagactta ctgcttgtat attcaaaggc tttaaaaact ctatcatttt tttaacctct
     3841 aaattttcat catctacatt tactcctaca attaatggta ttcttatatt tatgttagcc
     3901 cctaattcac ttaaaatctt aacatttttc agtattaagt catttgattt tcctgtaaat
     3961 tttatgtgct tatcttcatc aataagctta atatcatata aaaacaaatc tgcacattta
     4021 gcaactcttt cataattttc tgactttgca tagccacatg tatcaattgc tacatggatt
     4081 ccctttgatt tgcacatgtt tattactcca caaatgaagt ccatatcttg agccattact
     4141 tctccacctg atagagttac tccaccacct gattcttcat agaaaattct atccttctct
     4201 atctctttaa ccaaatctct tacagaatac tcttgaccaa caatctctct tgcattgttt
     4261 acacaatagt ctaagcaagt ttcacaaaac tcacacttat cttggtctaa gcatattttt
     4321 gtttcacctt tgtaaattgc cttatgaggg catatattta tacatgcttc acattttgaa
     4381 catctttctt cattatacaa aacttgtttt gtatatgttt gactctctgg attatgacac
     4441 caaacacatt caagtggaca ccctttaaaa aatactgtac ttcttatccc tggtccatca
     4501 tgtatgctac atttttgtaa gtttataact aatggattca tgatttttca cccctaaata
     4561 atatccattc cattttttca tttttatgca attcatcttc atagaatata cctatgaaaa
     4621 gcatttctac aattacataa tactatattt gttattattt tgtctatata tttttagtaa
     4681 tattaaa
//
LOCUS       DAMJVI010000175         4534 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.181, whole genome shotgun sequence.
ACCESSION   DAMJVI010000175 DAMJVI010000000
VERSION     DAMJVI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.181"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            241..1716
                     /locus_tag="PC545_003541"
     CDS             241..1716
                     /locus_tag="PC545_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2458254.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITLEKV
                     RTEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            1803..2216
                     /gene="speD"
                     /locus_tag="PC545_003542"
     CDS             1803..2216
                     /gene="speD"
                     /locus_tag="PC545_003542"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDF2458255.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            2246..3097
                     /gene="speE"
                     /locus_tag="PC545_003543"
     CDS             2246..3097
                     /gene="speE"
                     /locus_tag="PC545_003543"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDF2458256.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKEYMDICSCKLDDKRVSLYFEDGVNFVKCSHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            3087..3965
                     /gene="speB"
                     /locus_tag="PC545_003544"
     CDS             3087..3965
                     /gene="speB"
                     /locus_tag="PC545_003544"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDF2458257.1"
                     /translation="MKNNFYHMNTFMSMNKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVHEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFENIVNMLNGKNIYLTIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISDKIK"
     gene            complement(4131..4331)
                     /locus_tag="PC545_003545"
     CDS             complement(4131..4331)
                     /locus_tag="PC545_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2458258.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
BASE COUNT         1802 a          499 c          770 g         1463 t
ORIGIN      
        1 agtttataaa tactaagttt taaaaaaatt aatataaaca acaaaaaatt aaaaagttta
       61 taaatattga tatagctaat gtatatgttt ttgagtaact tcaaaaaaac acttgtaaaa
      121 atgctgggat gttaatataa taaatatcat gagtttaata aaagtaaaaa taaaaaatag
      181 aaataataaa taataaatat taaaaatatt tgaatataga gaggagatga atttgaaatc
      241 atggatagcg agtttgcaaa tgcagttgaa ttaagctatg agtgtgctcc attattagag
      301 tcgttaaagg aatactcaga gaaagatata gcttgttttg atgtgcctgg acatgtaaaa
      361 aatagaggtg tagctatatt aaataaatat ctaggtgaaa gtctcatgaa aatggatata
      421 aattcatcac caactatgga taatgtatct gcgccaaatg ggattataaa aaatgcacaa
      481 gatttgttag cacaagcata tatggctgat gaggcattct ttataacgaa tggcacaact
      541 caagcaatac atgcaatgat tttgagtgtt ataaaaccag gtgaaaaagt attacttcca
      601 agaaacatac ataaatcagt aataaatgcg ttaatacttt gtggaggaat accaatattt
      661 atacaaccag aatttgatga aaaactaggt ataagtttaa atattactct tgaaaaagtt
      721 agaacagaga tagaaaaaga ttgtaatata aaagcattat ttttccttaa tccaacttac
      781 tatggagttt gtgcagacct tgagagtatt atagaactat gccataaaaa taatgtttta
      841 gtgttagttg atgaagctca tggtgcacat tttccatttc atttagattt acctccttct
      901 gcaataagtt tgggagctga catggtagct gtaagtatac ataaaactgg aggagcattg
      961 acacaatctt cagcactctt attgaataga gataatgtaa gttttgaaaa ggttcttcaa
     1021 tcaatcaata tgctacaatc aacatcagca tcttatttgc ttatggcaag tatagatggt
     1081 gctagagtta atctagttga gaatggtgaa aagcaattgt caaaagcatt aaatctatct
     1141 aggtatgcaa aatctaaatt gaacaaaatt gatggaataa aagttttatc tacagaaata
     1201 ttgaaacaaa aaggtgttaa atttatagat gaaacaaaat tgtgtataaa tgtaaaagaa
     1261 ttaaatttga ctggatttga agtatacgat ttattatata aaaatttttc aattcaagta
     1321 gaattgggag attcatataa tatactggca cttgtgtcca ttggaacaaa taaatcagat
     1381 atagatagat tagtaaaggc actttctata attgcaaaga tttatagaaa agaatcaaca
     1441 ttaaatgaat ttaatatggt tcaaataaat ccaattatta aacttaatcc aagagaagct
     1501 ttttatgcac caaaggaaag tgtagaaatc aattcatgta tagatagaat ttgtggagaa
     1561 tcaataatgg catatccacc aggtataccg attattgctc caggagaatt gataacagaa
     1621 gaaattatgg agtatattat tttcttaaaa aatagcaatg catatcttac agatgtacaa
     1681 gataaaaact tagatagaat actagtaata aaataaatta ctttattttg caagggcaag
     1741 tgtttgttaa gtagagttta tcaaatagaa cttattaaaa gtataaagga gttggggttg
     1801 aaatgaaatt tgaaacacta ggaagacata tactagtaga atactataat tgtgatgaaa
     1861 acgtacttaa agacccacgt cttatagaaa attttatgaa tgattcagca ttaaatgcaa
     1921 aagcgacaat tgtggattct gtattccacc attttaatcc ttggggggtt tcaggagctg
     1981 ttataattgc tgaatctcat ttgacaatac atacatggcc tgaatatggt tatgcagcag
     2041 cagatttttt tacttgtggt gatatagacc catggaaaag ttttgaatta ttagagcaac
     2101 ttttaaagtc tgaaagaagc gagtctacag aaattccaag aggcttaact acaaaaatta
     2161 aaaaatattc aaaaaaagat ttaggtaaga ttactcataa accagaagca gtataaaaca
     2221 tacaagagaa agtgaggatt attatatgga actttggtat acagaagaat ggacagaaaa
     2281 tgtaagattt tctataaaag taaataaaca tctatttgaa ggaaagtctc aatttcaaag
     2341 gatagatgtg tttgatagtg atgaatttgg aaaatttcta acaatagatg gattaatgat
     2401 ggtaacatat aaagatgaat ttatatacca tgagatgata actcatgttc ctatggctac
     2461 aaatttaaac ataaagaaag tattagtcat aggtggaggt gatggaggta cagtaagaga
     2521 attaagccgt tatcctcaaa tagaaaagat tgacatggtt gaaatagata aaatggttgt
     2581 agatgtatct aaagagtata tggatatatg ctcatgtaaa ttagatgata aaagagtgag
     2641 tttatatttt gaagatggtg ttaattttgt taagtgttca catgataaat catatgacct
     2701 gataatagta gattcaactg acccaatagg accaggtgaa gggcttttct caactgattt
     2761 ttataaggat tgttatagaa tactgactga tgatggaatt ttagtaaatc aaagtgaaag
     2821 tccgtatttt gactttaatg ctaaagagat gaaaagagct aataaaaagt taaaacagat
     2881 atttccaata tcagaagttt atcaagcaca tataccaaca tatccatctg gtcactggtt
     2941 gtttggattt gcttcaaaaa aacttaatcc agtaaaaaat caagatagaa atggatggga
     3001 gaaattaagt ttaaaaacta aatattataa tagtgatata catttaggtt cgtttatgtt
     3061 acctcaatac gtgaaggaga tgctggatga agaataattt ctatcatatg aacactttta
     3121 tgagtatgaa caaaaattat gaagaatcta atcttatagt atttggtgtt ggatttgatg
     3181 gaactacttc taacagacct ggggctagat ttgcaagtag ctctatgaga aaagaatttt
     3241 acggtcttga gacatatagt ccttttttag atttggattt agaggattat aatatatgtg
     3301 attatggaga tttagaaatt agtgttggaa gtacagaaca agtcttaaaa gaaatctatc
     3361 aagagacata taagattgtt agagattcaa aggtaccctt tatgattgga ggagagcatt
     3421 tagttacatt accagccttt aaagcagtac atgaaaagta taatgatata tatgtaattc
     3481 attttgatgc ccatactgat ttgagggaag aatataataa tagtaaaaat tctcatgcaa
     3541 cagtaattaa aagaatatgg gatattgtag gtgataataa aatatttcaa tttggtataa
     3601 ggtctgggac aaaagaagaa tttaaatttg ctacagaaga aaaacacaca tacatggaaa
     3661 taggaggaat agatacgttt gaaaatatag ttaatatgct aaatggaaag aatatttatc
     3721 taactataga tttagatgta ttggatgcat ctgtgtttcc aggaacaggt acaccagaac
     3781 ctggtggtgt aaattataga gagtttcaag agatttttaa gattataaaa aactctaata
     3841 taaatatagt tggttgtgac attgtagagt taagccctga ttacgataca acaggtgtat
     3901 cgacagttat agcttgtaaa atcttaagag agttatgctt aataatatct gataaaatta
     3961 aatagagtat tttatataaa tagtatatat tataatataa gaagttattt taagtttgac
     4021 gcttttctaa agtaacttct tatttttgta attaaaaata aatataataa acaacaaaaa
     4081 agagccctcg atagctcctt ttctaaaata tatatattta aattaaataa ttatattcta
     4141 tttacgtttt cagcttgagg tcctctatta ccttgagtta tatcaaagct aaccttttcg
     4201 ccttcttcta aagttttgta tccatcattt tgtatagctg agaaatgtac gaaaacatca
     4261 tcttctccct ctacagatat gaatccaaat cctttttcat tgttaaacca ttttactatt
     4321 ccgtttttca ttataagaaa acctccgcat tattaaaaaa tacaaactaa aaaaataagt
     4381 ctgttctatt aatataacat aaattttaaa taaaagcaaa taaaattttt aaaaaaatta
     4441 attactttta gaatttaata aaaattgtta aattatacag tgagaagtat tgtaaaattt
     4501 gacgaaaatg taaatgtaga taataaattt aata
//
LOCUS       DAMJVI010000176         4518 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.182, whole genome shotgun sequence.
ACCESSION   DAMJVI010000176 DAMJVI010000000
VERSION     DAMJVI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.182"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(950..1360)
                     /locus_tag="PC545_003546"
     CDS             complement(950..1360)
                     /locus_tag="PC545_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458259.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEQEL
                     EKIKTEGLKIMKPITIENEDGSQISIAYNYAAID"
     gene            complement(1576..2958)
                     /locus_tag="PC545_003547"
     CDS             complement(1576..2958)
                     /locus_tag="PC545_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HDF2458260.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNTSTENYTLKKASDSTGKNNSDEFEIMSNIE
                     NIMMLEMLGILSFIVTIMFSILLCFRYFKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGTGLVFATENKEINEVESF
                     GLTKNEQSDKIGFSISSKSTDLYSPKVESDILVSKAKSDGETHFSTAKVSVIEFREHE
                     ESSMSKVTNSENSEETYVNKSSNGKDVLYTDSGEVSIEQSGYNDNRFVISRKGNLLAV
                     NKEQYRTWIE"
     gene            complement(2969..3328)
                     /locus_tag="PC545_003548"
     CDS             complement(2969..3328)
                     /locus_tag="PC545_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2458261.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(3533..4312)
                     /locus_tag="PC545_003549"
     CDS             complement(3533..4312)
                     /locus_tag="PC545_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDF2458262.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKIGDIFVSGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTVISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICEDKDNMGVFKSMGASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         1570 a          768 c          401 g         1779 t
ORIGIN      
        1 tatctaccat catccatata tttattttac gattactctt tcaccctaac cctttaacta
       61 tctatttttt cttactattc ttatccacat catccatata tttattttac gattactctt
      121 tcactctaac cctttaacta tctatttttt ctgatattct tatccaccat cacccatata
      181 tttattttac aattactctt ttaccctaac cctttaacta tctatttttt tctggtattc
      241 ttatctacta ttatctgtat atttatttta tgattactct tttaacctac cccctatact
      301 ttatagtaat atgtttttaa cctttttcat tattcccaat atgctttata actacaaata
      361 ctctcccccc ttaaaaataa taataaaatt aatcaataaa tttataatca tattattact
      421 ctatgtatta agcaattact atgccaataa actttgattt ttttaaacaa atcttaaaaa
      481 tcctaaaatg ctaattttct ttaactttta atgaatctaa tagataacat ttaattttcg
      541 ttatattttt cagaatattt ctattattat ttttaagaaa aaaaaataat gttaacattt
      601 cttaacatac attaaaaaat gttaacatta taaattgggt ttaatacttt tactttgact
      661 cgatttactt ttattctact tcttacttta tttttaactt agactttatt tttttattac
      721 ttatttgagt cttatttatt ttattatcac tccatatgtt caattacatt tgtctaaatt
      781 ctttgatact tttattacat aattttaaca agcttaagtg ttatttgggt agtgcagttg
      841 ataattattt aaataatcat caactgcatg tttatatcat tacagtagct taatgtattt
      901 aatcatactt acttaggatt ttgaaatatc ttagagatta tatttaacat tagtcaattg
      961 ctgcataatt gtaagcaatt gaaatttgag acccatcttc attttctata gtgataggct
     1021 tcataatttt taatccttca gtttttatct tttctaattc ttgttccatt ttcttaattg
     1081 tttcctcaag gtctttttga cttacatctt tgactgcatt agggtcttta gcatagtctt
     1141 ttaattcttg aatatgttta gcatactctt cataagtata atgctcaaca ccatactttt
     1201 ccaattcagt agggtcagtt attcctgatt ttattagaga ttcaagttta ggtaaagcaa
     1261 tattagatga atcatttcca tttgcaaata ctgctccagt tcctcctagt accattgcag
     1321 ataccatcat tgcacttata agggattttt ttattttcat aaccaatctt cctttcttag
     1381 agatttattt gggtagtgta gtttgaaatg actattgcaa tagttatcaa ccacatcctt
     1441 agactatcac agtagtcatg tttatgtcaa gcctttttaa aaaacttttc aataaaaaat
     1501 actatctcaa aacaaaatta attattttga gatagtattt taattctaaa tcttttatat
     1561 tactaagatt tatggttact ctatccatgt tctatattgt tctttattaa ctgctaacaa
     1621 attccctttt cttgatatga caaagcgatt atcattatag ccactttgct caattgaaac
     1681 ttctcctgaa tcagtataaa gtacatcctt accattacta gatttattta catacgtttc
     1741 ttcgctattc tcagaattag ttactttgct catagatgat tcttcatgct ctctaaattc
     1801 aattacagat acttttgccg tactaaaatg agtctctcca tcacttttag ctttactaac
     1861 aagaatatca gattctactt tgggagaata taaatcagtt gatttactac ttattgaaaa
     1921 tccaatttta tcactttgtt cattttttgt taaaccaaaa gattcaacct cattaatctc
     1981 tttattttct gttgcaaaaa caagacctgt cccacaaact aatacagttg aaactaaaaa
     2041 agtaacataa gttgattttt taaatttcat aatattaact attctctttt ctatttttgt
     2101 ctttccaaat ccactacata ttgtcattga aaatggatta ttctcctctg ctagacttat
     2161 tagtgtggtt gcataatcag ccctatattc ttcaccaagt atactcataa ccttttcatc
     2221 acaactaaat tctatgtctt tatttagtaa aaaaaacatc acccaaacta agggattaaa
     2281 ccaatggaca acaagagcta caagcataaa taactttaac agattatcaa agctattaat
     2341 atgaaccatt tcatgagtta aaacaaagtc taaagtttga ttattagcat aattcatttg
     2401 ctttggcaaa ataattcgtg gttttaaaat accagatgta agtggtgtcc tagtaaattc
     2461 agatatgcaa acaataaatc ttctaaaacg gctattctta cacatccatt ttttaatgaa
     2521 ctcatgattt tttatatgaa ttgaagtttt aaaaagatta attattttaa aatatctaaa
     2581 gcataaaagt atgctaaaca taatagtaac tataaaagac aaaatgccca acatttccaa
     2641 catcataata ttttcaatat tagacattat ttcaaattcg tcagaattgt ttttacctgt
     2701 agagtcacta gctttcttaa gagtataatt ttctgttgat gtattaccca attccacatc
     2761 aaaagtcgat tcaattatac tataaatact aaaaccagaa tttaaactta ctggtattag
     2821 aagtttaaat aaaacaatca accacaataa tacaaatacc tttttaggta attttttaaa
     2881 taaacatgct cttataaaaa taactattaa aagtaatact ccagaagtaa aactcatttc
     2941 taacaattta atgaacataa atcttacctc actttaattt ttcaacaatt tgtttaagtt
     3001 ttgtaatctc atcacttgaa agattttctt tattaataaa agctgaaaga aatttttctg
     3061 aagaccctcc aaacatttta ttaattaaag tttctgtttc ctgtttttgt acttcctctt
     3121 tgcttattaa agctttacaa acaaaattcg gctcaattct ctctattgca ccttttgaca
     3181 ctaatttttt tataacggta tacgtagtgt ttctattcca tcctgtttct tcttttaaaa
     3241 tttttactaa ctctccagcc ttgctttctc ctctctccca tagaacttcc attactttaa
     3301 gttctgaatc aaataatttt atctccatat aaaatcgctc ctcaaatata gttatcacaa
     3361 tagtcattac tataataaaa aactatcata gtagtctata ctatgtcaat catttatttt
     3421 ttaataataa aaactgtctt tttaaaaaat tcttataaaa gacagtttta tcaatgttat
     3481 actttaatct ttaattaaag ccatatttta tttaaatata taactatata tcttagtaca
     3541 tagtataaga tttttcttta tctaatagta tatcctctac atctgggttt tcttttacta
     3601 attgtctata aaaattacaa ttagtaacac ctacatatgg aattagccat agtagaccta
     3661 ttccaagagt agctattgaa agtatatacc atcctataaa actaaagttt agtacaaata
     3721 atttaagtac atttcctttc atcatatcta aactagcacc cattgattta aatacaccca
     3781 tattatcctt gtcttcacaa attaagaaag tagcttgaga aacatataat agcaatatat
     3841 aaaccggtaa cataagaaca agtattataa gaactagagt tattccagta gaaataacag
     3901 tccctattcc aacattatca cttgataaat attcaattgc tccactagta agtaaaactc
     3961 caaatgataa aaacgctcct ataattgtga ttataaaaga ataaatataa acaataattg
     4021 attcaaataa ataaaaacca aaacttctta aataaattct cccacttaca aagatatctc
     4081 ctatcttgac tttttcatct ttagtaattt tcaagaacat acatgtaaat cctgctgaaa
     4141 cgcaaaaagc tattacagcc actacaatag tagtcaatgg ttcaaaaggt aatcctacaa
     4201 ttgatattaa tactatcaat gcaatatata ctgccataga aattgcacat atcccccagt
     4261 ttccagataa ctgagcttta gatactcgtt ttaattcttg ccttgatacc ataaaaaaca
     4321 cccctttcat tttataaaaa aacttttaaa aatataaata atcttttaac gtataatatt
     4381 aatatatcat attttcgact atataagata caattattca ccatgtttta tcaagatata
     4441 ccataattgt attactagtt ttatatagtc aacattacca aatacatcaa ataaatttga
     4501 ttaattattc aacaagta
//
LOCUS       DAMJVI010000177         4463 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.183, whole genome shotgun sequence.
ACCESSION   DAMJVI010000177 DAMJVI010000000
VERSION     DAMJVI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.183"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            152..1750
                     /locus_tag="PC545_003550"
     CDS             152..1750
                     /locus_tag="PC545_003550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HDF2458263.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDIKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VVSVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEIMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLILSVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1985..4066)
                     /gene="bclA1"
                     /locus_tag="PC545_003551"
     CDS             complement(1985..4066)
                     /gene="bclA1"
                     /locus_tag="PC545_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA1"
                     /protein_id="HDF2458264.1"
                     /translation="MRNIILYLNDDTFISKKYPDKNFSNLDYCLIGSKCSNSFVKEKL
                     ITFFKVRIPDILKDKSILKAELFIHIDSNKNHIFKEKVDIEIKRISEYYNLRTITWND
                     RVSMENIRGYLPIGISDTSNYICLNITGTIKAWAMNKYPNYGLALSLNYPYQILEFTS
                     SRGCNKPYILVTFEDRIIDNCYPKCECLPIRITGPMGPRGATGSTGPTGVTGPTGSTG
                     ATGSIGPTGPTGNTGATGSIGPTGVTGPTGSTGATGSIGPTGVTGPTGNTGVTGSIGP
                     TGATGPTGVTGEIGPTGVTGPTGVTGSIGPTGATGPTGATGVTGEIGPTGATGPTGNT
                     GVTGATGPTGNTGVTGVTGATGPTGATGVTGEIGPTGATGPTGEIGPTGATGPTGATG
                     VTGEIGPTGVTGPTGEIGPTGATGPTGVTGSIGPTGATGVTGEIGPTGPTGVTGSIGP
                     TGATGPTGNTGVTGVTGPTGATGPTGVTGEIGPTGATGPTGVTGEIGPTGATGPTGPT
                     GNTGATGSIGPTGVTGPTGATGATGVTGPTGPTGATGNSSQPVANFLVNAPSPQTLNN
                     GDAITGWQTIIGNSSSITVDANGTFTVQENGVYYISVSVALQPGSSSINQYSFAILFP
                     ILGGKDLAGLTTEPGGGGVLSGYFAGFLFGGTTFTINNFSSTTVGIRNGQSAGTAATL
                     TIFRIADTVMT"
BASE COUNT         1323 a          789 c          686 g         1665 t
ORIGIN      
        1 tatattaaat ttgtagtatt tatgtaagaa ataaatttta tataatattt tttcactaaa
       61 tttttttctt tttacaacaa atactcacta ccttttgata taatattttt atcaattatt
      121 attatattca agttttgaaa ggagtattaa aatgagttta ttagttttcg gacataaaaa
      181 tccagataca gattcaatat gttctgcaat ttcattagca catttaaaga acaaactagg
      241 aataaaagca acagcttatg ctttaggaga tatcagaaaa gaagctaaat atgccttgga
      301 ttactttaag gtagatgcac ctgaaatttt agataatgtt agaatacaag ttagagattt
      361 aaattatgat aaagtagtac ctttaacacc aacttcatct atattagagg cttataacct
      421 tatggatgaa aaaaatgtta aaacactacc tgtaacttat gatgatggaa cttttgctgg
      481 tatcattact atgaaagaaa tagctaaaaa tctacttcat caacacttta caactataca
      541 cacatcacta tcaaacattt gtaaaaacct taatggtact attcttgtgg actgtggtga
      601 agatataaaa ggtagagtag tcactttatc atttggaatg gagactctaa tagagacttt
      661 aaaagaagga gacatagcta ttgttggaga tagatatgat agtattgaat atgctattga
      721 tacaaaagtt aaacttttaa tacttactaa tcatacagag atttcaaaag atttgctagc
      781 tcttgctaag ataaatggag tgtctgttgt ttctgttgaa agtgacaatt ataaaacttc
      841 taatgtaata aatcaatgta gtttcttaga ctctatagaa gctacagaaa atttaattac
      901 attcaaagaa gatgattata tggatgaaat aaaagagatt atgctagaaa caaacttcag
      961 atcttatcca atattagatg atgataataa gtttttaggt ttagtttcaa aaggtcatct
     1021 attaaaccca tctaaaaaga atgttgtttt agttgaccat aacgaatatg ctcaaagtgc
     1081 tgatggaatt gaacaagcaa acatagttga aataattgac catcataaac ttggcggtat
     1141 ttctactgat gttcctatgt catttagagt tatgcctgtt ggatgcaata gtactataat
     1201 ttatcaaatg tacaaagaaa ataatgttga gataccttat gaaatagctg ggcttctttt
     1261 atctgctata ttatcagata cattattatt taagtctcca acaacaactg atatggataa
     1321 gaaagcttgt gaagaattaa gtaaaatagc aaaagtagac atggaaaaat acgctatgga
     1381 tatgtttaaa tgcggtactt ctttagatga atatactata gaagaaatag taaatatgga
     1441 ctttaaagaa tttaatatga gtggacatag agttggtatt ggacaagtat ttaccttaga
     1501 catagactct atatttgcta aaaaagatga gtttttatct tatataaatt cgactgatta
     1561 tgataaatta attctttcag ttacagatat aatcaaagaa ggttcttatt taatctataa
     1621 agcagaagat agcttaattg ctaacgcatt tggtgttgaa ggtgttcaag gcacatttgc
     1681 accaggtgtt gtatcaagaa aaaaacaatt agtccctaat ttaactactg caatcaaaaa
     1741 ttttaaataa tataaaattt ttaaataacg aaaactgtct caaatctata tttgagacag
     1801 ttttttatta tatatatatt gataataaag ttaaataaaa atccataaat ttaaaatttt
     1861 tatgaatata ttttattatt attgttattt tattcattct aaaataggta aaactcaata
     1921 tataaaaata cttttgtcat ttaaataaaa cctaaatagt ttttaagatt attttagaca
     1981 cgttttaagt cataacagta tcagctattc taaatatcgt caaagtagcc gcagttcctg
     2041 ctgattgccc atttcgtatc cctactgttg tagatgaaaa gttatttatt gtaaaagtag
     2101 tcccaccaaa taaaaaacca gcaaaatatc cagaaagtac tcctccgcct cctggctcag
     2161 tagtaagccc tgccaaatct tttcctccta aaattgggaa taggatagcg aaagaatatt
     2221 gatttatact tgatgaacct ggttgtaatg ctactgaaac tgatatataa tacacaccat
     2281 tttcttgtac tgtaaacgta ccatttgcat ctactgttat acttgaacta tttcctatta
     2341 ttgtttgcca acctgttata gcatctccat tatttagtgt ttgtggagat ggtgcattta
     2401 cgaggaagtt agcaactggc tgagaggaat tgcctgttgc tcctgttgga cctgttgggc
     2461 ctgttactcc tgtcgctcct gttgctcctg ttggacctgt tactcccgtt ggccctatac
     2521 ttcctgtcgc tcctgtattt cctgttgggc ctgttggtcc tgttgctccc gttggtccta
     2581 tttctcctgt tactcctgtt gggcctgttg ctcccgttgg tcctatttct cctgtcactc
     2641 ctgttgggcc tgttgctccc gttgggcctg ttactcctgt cactcctgta tttcctgtcg
     2701 gtcctgttgc tcccgttggt cctatacttc ctgtcactcc tgttgggcct gttggtccta
     2761 tttctcctgt cactcctgtc gctcctgttg gtcctatact tcctgtcact cctgttggtc
     2821 ctgttgctcc tgttggtcct atttctcctg ttgggcctgt tactcctgtt ggccctattt
     2881 ctcctgtcac tcctgtcgct cctgttgggc ctgttgctcc cgttggtcct atttctcctg
     2941 ttgggcctgt tgctcctgtt ggccctattt ctcctgtcac tcctgtcgct cctgttggac
     3001 ctgttgctcc tgttactcct gttactcctg tatttcctgt tggacctgtt gctcctgtta
     3061 ctcctgtatt tcctgttgga cctgttgctc ccgttggtcc tatttctcct gtcactcctg
     3121 tcgctcctgt tggacctgtt gctcctgttg gtcctatact tcctgtcact cctgttgggc
     3181 ctgttactcc cgttgggcct atttctcctg tcactcctgt tgggcctgtt gctcccgttg
     3241 ggcctatact tcctgtcact cccgtatttc ctgtcggacc tgttactcct gttggtccta
     3301 tacttcctgt cgctcctgta cttccggttg ggcctgttac tcccgttggc cctatacttc
     3361 ctgttgctcc tgtatttccg gttgggcctg ttggtcctat gcttcctgtc gctcctgtac
     3421 ttccggttgg gcctgttact cctgttgggc ctgtacttcc tgtcgctcct cttggtccca
     3481 ttggacctgt aattctaatt ggaagacact cacatttagg ataacaatta tctataattc
     3541 tatcttcaaa tgttacaagt atatacggtt tgttacaacc tctactagat gtaaattcaa
     3601 gaatctgata agggtaattt aaagataaag ctaacccata attaggatat ttattcattg
     3661 cccatgcttt tatagttccc gtaatattta aacaaatata gttggatgta tcacttatcc
     3721 caattggtaa atatcccctg atattttcca tagacactct atcattccat gttatagttc
     3781 gtaaattata atattcactt attcttttaa tttcaatatc tactttttct ttaaaaatat
     3841 gattcttatt tgaatcaata tgaataaata actctgcttt taatatactt ttgtctttta
     3901 atatatctgg tattctcact ttaaaaaaag taatcaactt ttcttttaca aaactatttg
     3961 aacatttact tcctattaag caataatcta aattactaaa gtttttatct ggatattttt
     4021 tagatataaa agtatcatca tttaaataaa gtataatatt tctcatttat aatccctcca
     4081 ttttgggtct atatcatact atgtttaaaa tacatgctta gttacaatac aattaaaatt
     4141 tatagtatta aatttttata taataatgct gatttctaca aaaaaagaac atgtcattag
     4201 aaatttctaa tagcatgttc tttttcatta ttatatatta cactttaaat tattccttga
     4261 ctggcttagc tggtgtaaac taaaccagtc tttctaaaat attaagcact tttttcttat
     4321 tatgtttttt aggatttatt gtctatatta ttgtaaacca aagttataaa attgtaaata
     4381 gaatatatta ccataacaat atttttttat aagtaaaaat tataaaaaac tttatttttg
     4441 ttaaatggaa taaataaaat aag
//
LOCUS       DAMJVI010000178         4391 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.184, whole genome shotgun sequence.
ACCESSION   DAMJVI010000178 DAMJVI010000000
VERSION     DAMJVI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.184"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(243..734)
                     /locus_tag="PC545_003552"
     CDS             complement(243..734)
                     /locus_tag="PC545_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDF2458265.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDANENSTINIDKA
                     WHAIHFTLTGCPFGGDDDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQAFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKKFIQEASNDGLAVIF
                     FIM"
     gene            complement(1954..4089)
                     /gene="rnr"
                     /locus_tag="PC545_003553"
     CDS             complement(1954..4089)
                     /gene="rnr"
                     /locus_tag="PC545_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDF2458266.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIVSPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLP
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEILARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYIYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
BASE COUNT         1385 a          732 c          394 g         1880 t
ORIGIN      
        1 tagggtattt agccttttaa gataaataaa aatatctgca actatatatt gcaaaaattt
       61 aaaactaatt tatatatgct aatataaata tttataaaga cataagacaa atttacctac
      121 agatttaaat ttaacttata tattttaaaa ggctaatata atatgtattt tcttctatac
      181 aatcaaaagg cttcatatct aagatatgtg atgacactaa tattcacaat ttttagtaat
      241 tatcacatta taaaaaatat tacagctagt ccatcattag atgcttcttg tataaacttt
      301 ttaagctcaa ttagatttgc ccatacatat tcaaaaaaat catcttcact ctcatcattc
      361 ataacaggat aaatattatt ttctaacatc tcattaatat taaatctttt tctaaatgct
      421 tgttcttcca aactgttcat tgcttttgat agttttttta catcatttgc tgtaatcaac
      481 atgacaggaa attctccatc aatttccata agtatgtttc cacttagtac taatttgcta
      541 aatatattat catcatctcc accaaaagga caacctgtca aagtaaagtg aatggcatgc
      601 catgctttat caatattgat agtactattt tcgttggcat cttcaaaaac aaaatcttgt
      661 aaattttctt ctgcttgctg taatttaaaa acattctctt cacttatttt agtataacat
      721 cctatcattc ccataattac attcctccta agtagcgttt ttttaattgt aacttataat
      781 taaacttagt tacaataaaa tatattgaga aaagtattat ttcatcttaa atttaaatac
      841 ctatattatc aactaaaatt ctgtgtttat agcgtaagta ttattttttc ctaaaaatta
      901 aatgaatcag aggttctata ggcaatattg actataaata agaactatta ctttttaaaa
      961 aaaatttaag aaattatact gtagaatcat gctctactta ttagctttaa ataaaaaata
     1021 aatcatgttc tcaaattaaa taataaataa caataaattt aaattttaaa ctataagagt
     1081 ttctgaagtg ttaataagca taccaataat tatatgtgta agaatttgat ttaatagata
     1141 tgaataattt ttacattaat tataaatata ttaacctatt gttataaatt ctaaaaaaga
     1201 aaagttatct taaataaaaa ataagtgtat cttaaatttt aaatgataca cttattttta
     1261 tattgaaatt atatttttta atatctattc ttaattaggt atttttaaat tatctgttta
     1321 cattaaatat ttcttaatta cttatctttc agctagcttt ttctattaaa tgtttctcaa
     1381 ttacttattt ttcaattagc cacttctatt aagcatttct taattactta tttttcaatt
     1441 agtcacttct attaaacatt tcttaattat ttatttttca attagccact tctattaagc
     1501 atttcttaat tacttatttt tcaattagcc acttctatta agcatttctc aattacttat
     1561 ttttcaatta gccacttcta ttaagcattt cttaattact tatttttcaa ttagccactt
     1621 ctattaagca tttcttaatt acttattttt caattagcca cttctattaa acatttctta
     1681 attatttatt tttcagttgg ctttttctat taaatgtttc ttaattactt atctttcagt
     1741 tagctctttc tattaaacat ttctcaatta cttatttttc aattagctac tttcattaaa
     1801 tatttcttaa ttatttatct ttcagttaac tatttccatt agatatttct caattaccta
     1861 tttttatcat ttattttata gagtattgtg cactatatat ttatacatat cataaatact
     1921 acaacttgtt atctcttatt ttagctgttt tatctactcc tctatttttt caactatctc
     1981 aaagtcaatt tctttaaaat ctacattaac actttcaacc tttatcttaa caaggtcacc
     2041 tatcttatat gacttcttag ttctttctcc taaaacacta taagaactct catcataaat
     2101 atagtaatca tcattcatat ttgcaagtct tatcaaccct tctatggtat tatctaactc
     2161 aacaaatatt ccaaatgaag ttacgctaga tacaacaccc tcaaattctt gaccaacttt
     2221 atccttcata taaagagcct tatagaaatc atgtacatct ctttcagcta actctgcctt
     2281 tctttctttc tcggatgatt gaacagatgc ataatccact atagtagtaa gctgttcttg
     2341 tctttttcca cttattttat tatttaaaga ttcttttatt attctatgaa tttgaaggtc
     2401 aggatatctt cttattggtg aagtaaagtg acagtagaat ttagctgcta atccaaaatg
     2461 tccactacat tctggtgagt acttagcttg ttttaaagaa cgaagcatta tagtactgat
     2521 agctccttct tcttttttgc cctttatttt ctctattata ccttgtaatg cttttggatg
     2581 tacttcctct ttatcacctt ttattatata tccaaatgta gatataaact tactcaattc
     2641 ttccattttt tccaaagatg gtgtctcatg tattctatac acaaacggta ttcccatcca
     2701 gtaaaaatgt tcggcaattg tctcattact tataagcata aattcttcta ttatcttatt
     2761 agatattctt ctttcatatg gctttatatc tacaacttcg ccttttccat ttagaattat
     2821 ctttgcctct gggaaattga agtctatagc tcctcttttt tcccttcttg acataagaat
     2881 cctagctaaa atttcagcat ttttaaaatc ttcaacaaca tgttcaaaag tctttttaag
     2941 tttttcatca tccttttcta atatatcaga tacttctgta taagtcattc tagcttttga
     3001 atttattaca gattcacata tttcatgttt aaccacattt cctttatggt ctatttccat
     3061 aaatatagaa agagttagct tatcttcaaa tggatttaag ctacatactc catttgaaag
     3121 agtttttggc aacattggaa ttactttatc tactaaataa actgatgtag ctctttttaa
     3181 ggcttccttg tcaagtttat ttttctctct tacataatgt gtaacatcag ctatgtgaac
     3241 tcctagtttg aagtttccat taggtaatac ttctattgat atggcatcat ctaagtcttt
     3301 tgcgtcatct ccatctatag taaatatgtt taaatctctt aaatctcttc ttctttttat
     3361 ctcttcttga ggaatctcaa cagctacagc ttctgcttct tgcaatactt tctttggaaa
     3421 ttcttctgga agaccatgtt ctcttattat agaatctatt tctacgcctc tttcaccttt
     3481 ttgacctagt atctctataa ttttgccttc tggttttcta tcctcttgtg gccatactgt
     3541 aatttcacat actaccttat cattatctac tgctccacta aaatgttttt taggtatata
     3601 tatatcttta gtaaatttct tgttgtctgg tgttacaaat ccaaaagttt tacttgattg
     3661 gaaggtacct actattctag ttatttctct ctttattact tttactattc ttccttctgc
     3721 tctcttttct tcagttgcag gtactactat ttctgccata actctatcat tatgcatagc
     3781 accatttata ttgtcacttg agataaataa atcctgtgta tattcaacat cagattctac
     3841 aaatccaaaa ccctttttgt gtgatacaaa ttttccaaca aagtatccca tttgattggg
     3901 agatactatt tttccttttt tagtaaagca gatataacca tcttcttcta attcatttaa
     3961 aaaattataa aacattggca tttctgatga atgtatatca aatatttctg ctaattcttc
     4021 tttttttaga ggattatatg cactttcatt tattaatcct agcaaacttt ctttaagtcc
     4081 tggtatcatt tttccctcct gttttacttt tcattatatt attatttata ttgtctttta
     4141 tatttcttca gacaaaacat tttttactta tcacataatt ttctatattt tcttattatt
     4201 ataagttaaa attacttatt tatatttttg atttaatgta atctttgtca tactaacttc
     4261 aaacaaatta tgatgtttta taaattattt gtaaatatca ttataattac ttaagttatt
     4321 ctttatcata attgcttaaa ttactcttta tcacctaatt atacaccatc aaataggatt
     4381 ggtaaaatat t
//
LOCUS       DAMJVI010000179         4239 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.185, whole genome shotgun sequence.
ACCESSION   DAMJVI010000179 DAMJVI010000000
VERSION     DAMJVI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.185"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            225..1844
                     /locus_tag="PC545_003554"
     CDS             225..1844
                     /locus_tag="PC545_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDF2458267.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPVLGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLVLNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDEHFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFRNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            2218..2412
                     /locus_tag="PC545_003555"
     CDS             2218..2412
                     /locus_tag="PC545_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458268.1"
                     /translation="MINYMVEIIYSEKISEVENMLKIENIQYEFTSRDGFIFFGFYDM
                     TDAYRFETLCSDEKIRFKRI"
     gene            complement(2971..3294)
                     /locus_tag="PC545_003556"
     CDS             complement(2971..3294)
                     /locus_tag="PC545_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458269.1"
                     /translation="MATEIFTVKITPLEAQKMIKENLDADLVFSDSYNLGDDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKATSTGSAIGILKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(3339..3593)
                     /locus_tag="PC545_003557"
     CDS             complement(3339..3593)
                     /locus_tag="PC545_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458270.1"
                     /translation="MENKDFKLTICIFAFVIGVVVLFCCIGLGEFSAEHIIQQNGGIM
                     DTDQYNIYLDQSISQYRNFGSILALLGGIGILFDKYIRKY"
     gene            complement(3857..4138)
                     /locus_tag="PC545_003558"
     CDS             complement(3857..4138)
                     /locus_tag="PC545_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458271.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
BASE COUNT         1544 a          557 c          489 g         1649 t
ORIGIN      
        1 atactaaaaa tctaagttca agtatttaaa ttgctatata tcataaattt tctaaaatta
       61 acaacaactt aaaaatttaa cattctctta acgtatactt aacaaaacat taatattttt
      121 attgacaaaa tttgttattc tatattatgg aaacaataaa ttttagttaa taaagtttat
      181 aattcagata aaaaaattat taatttctta agggaggaaa tatcgtgaat atagtaatta
      241 gccttatcgg tggacttgga ctttttcttt acggaatgag tttaatggga gaaggtttac
      301 aaaaatcagc aggagacaaa ttaaaaaaaa ttattgaact cttaacaagt aatgttgtta
      361 tgggtgtact ggtaggtaca gtcgttacag gtattataca aagttcaagt gctactactg
      421 ttatggttgt aggatttgta aatgcaggta taatgaatct aagtcaagct attggtgtaa
      481 taatgggggc aaatataggt actacagtta ctgctcaatt agtttcattt aatcttgaag
      541 gaattgcacc aattgcttta ggtataggta ttttattcta tttatttaca tcaaatcaaa
      601 aaacaaagca tttatctgag atacttatag gttttggtat attatttaca ggaatggagt
      661 ttatgaaaga tgcagtagct ccacttgctg aatacaaagc attcactgat gcacttttat
      721 attttagtaa aaatcctgta ttaggtatat tggcaggatt tgccataact ggtattgttc
      781 aaagttcaag tgcatctatg ggtatgttaa tagcactagc ttctcaagga atacttcctc
      841 tatcatctgc tttacctatt ctttatggag ataatatagg tacttgtgtt acttcacttt
      901 tatcaagtgt tggtgcaagt agaaatgctc gacgtgcagc tgtaatgcat ttaagtttta
      961 atgtaattgg tacaataata tttatgcttg ttttaaataa acctatatca gccatagtta
     1021 ctcattttga cccaactgat acagctagac aaatagcaaa tgcacatact ttatttaatc
     1081 ttacaaatgt tataatatta ttaccatttt ctaaatacat agttaaatta gcaaatagac
     1141 ttatacctat taaagaaact gaatcagaaa tagtaaataa tactaagtac ttagatgaaa
     1201 ggatgtttag cactccatct atagcacttg gtaatactgt acaagaagtt gtaagaatgg
     1261 gtcataaagc tacaaactct ttggaacatt caattgcagg atttttaaat aagagcaatg
     1321 aagatattaa taaagctttt gagtcagaaa aagttgtcaa taagttacag aaagatatat
     1381 tgaactattt gttaaaactt tctaaagaac ctcttagaga tgatgaacac tttagaacag
     1441 atttgctttt taatactgta aatgacattg aaagagtctc tgaccatgca gaaaatatag
     1501 ctgaattggc catgagtgtt aaggaaatga acattagttt ttctgatagt gcgataagag
     1561 aaatatacga aatatacaat aagacaatta ctaattttaa ggatgcctta atagttttag
     1621 atgttaaaga ttttgagtta gcaaataaag ttttagaagt tgaaaatgaa gtaaattatt
     1681 tagaaaaaac ttttagaaat tcacatatga ttagactaaa taatggaagt tgtacaattg
     1741 atgctggtgt actttatctt gacttactta ctaatctaga aagaatttct gaccactcaa
     1801 caaatatagt aaaacaagta ctaaaactta aacaaaaaat atagtgatta tttttatata
     1861 taatcttaat caatgagaag taagaagcta tagataatgc ttatatcatt tatggtttct
     1921 tattttttat gtaatactca aatttttata gtattctctt attattaaac aatttctgcc
     1981 tagaatattc catatatata tgacttcttt cataaaactt gaactgccca taactaaagt
     2041 cacaagtata cataactatt ttttaatcaa ttataaccaa tatttgtttt atttatcttc
     2101 attttaattc ttcattcaat aatgtaataa gattattttt tatttggata ctattatatc
     2161 atctaaatat gttattatat atttggatat tttcatatta ataggaggga ttctacaatg
     2221 ataaattata tggtagaaat tatatatagc gaaaaaataa gcgaagtaga aaacatgttg
     2281 aaaattgaaa atatacaata tgaatttact tctcgtgatg gctttatatt ttttggtttt
     2341 tatgatatga cagacgcata tagatttgag actttatgtt cagatgaaaa aattcgattt
     2401 aaaagaatat aatagataaa aaagtacata tttctaaact atttgttttt caaatttagg
     2461 ttgtgtttta atgtagtgtt gatattataa acaattttaa taataaaaaa gtagggacat
     2521 tatattaata tgctcctact ttttactcat acttattaat attatttatc aaaattaccc
     2581 tacaaactgg aatttatcat tctttcataa ttttaaacta tcatatttaa aatatataaa
     2641 attccacatg atttttgtat aaacataata tcaaccatta tccaatttaa aaatattttt
     2701 tagtaatttc catttcattt tctcttaaca tattaattaa atttttatat gtaaatggtg
     2761 tatttacata ttatttaata tccaacaatt atataaagaa atgtgctctt tgccttataa
     2821 agaattataa agccaattcg ttttattata ttatttttct ttatacctta aaaactttac
     2881 cttcaaattc ttatttatct atatattata aaaatgttct taacctcttt tacgagattt
     2941 tcttgacata ttatcagtta gataaaataa ttaaataata taatcagcaa gtatgttttt
     3001 tactctttta agtaaatttc cacctgcacc ccaatcaatt tttaaaattc caattgcact
     3061 gcctgttgaa gtagctttaa taactgtttt tcctgtggta ttttcataaa ctactattaa
     3121 tcctgcatca ctattatttc ttgcataata cttctcaaaa gttgtaatta gtataaattt
     3181 atcatctcct aaattataag aatcactaaa aactaaatct gcatcaagat tttcttttat
     3241 cattttttgt gcctctaagg gtgtaatttt tacagtaaat atttctgttg ccatttttta
     3301 tctcctcata agaatttatt ttagacaaaa cataatctct agtattttct gatatattta
     3361 tcaaacaata tccctattcc tcctaacaga gctaatatag aaccaaaatt tctatattga
     3421 gaaatagatt ggtctagata aatattatat tggtcagtat ccatgattcc accattttgt
     3481 tgaataatat gctctgcact aaattcacct aaacctatac aacagaataa tactactact
     3541 ccaattacaa aagcaaatat acatattgtt aatttaaaat ccttattttc catagttatc
     3601 cctctcataa gctaaattta tttgattatt tttactagat tttatattac tcttattata
     3661 caatacaaca tatatttaca tctatttcca aaattgtaac ctttatagtt ttatatattt
     3721 aaatttttta aacaaaaaat acacatctat aaattataaa tgtgtattct gtaccattac
     3781 taataatata ttatactttt ttacaaattt ctaattattt ataactattc aaaaataaaa
     3841 ttcatataaa ataattctat tgtacaattg aaactttaat aatgtctcca ttttttctct
     3901 ccaagtgaac tacttctcca gttaaatcat agtcaaatgc cttaactaat atttcagtaa
     3961 cttttttacc atctactaca tcatcttttc cattaaataa atccaaggca ttattctcaa
     4021 tcattctctt tgcaaaattt acaggtaact ttatatgtat tttatcgcct gtatgtgata
     4081 atactcttat tcttaaaaac ttttctaaat tgtttcttgg tgtttgtttt gatgccatac
     4141 tattaaatca ctctccttta ttttttattt aataataaat tttattctta aaaatatctt
     4201 tatatgtatt tattatgaaa gtaacactat tattttcta
//
LOCUS       DAMJVI010000180         4220 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.186, whole genome shotgun sequence.
ACCESSION   DAMJVI010000180 DAMJVI010000000
VERSION     DAMJVI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.186"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            88..1782
                     /locus_tag="PC545_003559"
     CDS             88..1782
                     /locus_tag="PC545_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDF2458272.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLSCVEDINNSRNYKEQQKNIHKIN
                     LLRNKIETLSNIASIRYSTDTFNKFYKEEKNYWDEYMPLYEELNSYFYNAIVNSKFKY
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKTRELANKAYYNFFEENETKFDDIFDKLVKLRDKMSKKLGFEDFVEL
                     GYVRMMRSDYREYMIKNVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIKNGKRMYSELSKETNEFFDFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSSIKFIPYGVIVDEFQHYIYKNPNVDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLERGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DAGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
     regulatory      2114..2224
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2328..2699
                     /locus_tag="PC545_003560"
     CDS             2328..2699
                     /locus_tag="PC545_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HDF2458273.1"
                     /translation="MIIITNNPKVKEEVQGREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVILKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            2796..3743
                     /gene="trxB"
                     /locus_tag="PC545_003561"
     CDS             2796..3743
                     /gene="trxB"
                     /locus_tag="PC545_003561"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDF2458274.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            3847..4164
                     /locus_tag="PC545_003562"
     CDS             3847..4164
                     /locus_tag="PC545_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HDF2458275.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
BASE COUNT         1755 a          390 c          785 g         1290 t
ORIGIN      
        1 ataataattt ataaaagagt attacatagt acatgtaaat ctatatttat aaatacaata
       61 aagtaaagtt gtaaaggagt gctttaaatg aagttttcag agtttaagta tgaaagaccc
      121 aattataata gtatgaaaaa agagtttttg agttgtgtag aagatataaa taattctcgt
      181 aattataaag agcaacaaaa aaatatacat aagattaatt tattaagaaa taaaatagaa
      241 acgttgtcaa atatagcttc gataagatat agtacagaca catttaataa attctacaaa
      301 gaagaaaaaa attattggga tgaatatatg cctttgtatg aagaattaaa ttcatatttt
      361 tataatgcca ttgtaaattc aaaatttaaa tatgatttga taaaagagtt tggagagcaa
      421 tttttcacaa tagtagagta ttctttaaaa agtttttcca aagaaataat aagtgaattg
      481 caggaagaaa ataaattgtg ttcagagtat actagacttt tagcttcagc tgagataatg
      541 tttgatggga aaataagaaa tttatctggt atggggaaat tcatgtattc taagaataga
      601 aaaacaagag aattagcaaa caaagcatat tataatttct ttgaagaaaa tgaaactaaa
      661 tttgatgaca tatttgataa attggttaaa cttagagata agatgtctaa aaaattaggt
      721 tttgaagatt ttgtagagct tgggtatgtt agaatgatga gaagtgacta cagagaatat
      781 atgataaaaa atgttagaaa acaagtacta aaatatgttg ttccaatggc aaatgagctt
      841 tatgaaaaac aagctaaaag aattggtcta gaatatttaa gttacataga tgaaggcgtg
      901 gaatttttaa caggaaatgc aagtttaaag ggagattcaa gatacataat taaaaatggt
      961 aaaaggatgt attctgaact gtcaaaagaa acaaatgaat tttttgattt tatgttagaa
     1021 aatgaactta tggatttaga aacgaagaaa ggaaaaggag ctggagggta ttgtacttat
     1081 ataccagatt ataagtcacc attcattttt tctaatttta atcaaactgc tgatgatata
     1141 gatgtattaa cacatgaggc aggacatgca tttcagttgt atatgtctag atggattgat
     1201 atgccagaaa ttaattttcc aacgcttgat agttgtgaaa tccattcaat gagtatggaa
     1261 tttataacat ggccgtggat ggatttattt tttaaagaag atacagataa gtacaaattc
     1321 actcacttat catcttcaat taaatttata ccttatggag taattgttga tgaatttcaa
     1381 cactatattt acaaaaatcc taatgtagat aagtcaaaac gaaaagaaat ctggagattt
     1441 ttagagaaaa aatacttacc tcatagaaaa tatggtgata attctttttt agagagaggt
     1501 tgttggtggt ttaaacaagg acatatattt aaaaatccgt tttattatat agattatgta
     1561 ttagctcaaa tttgtgcgtt acaattttgg aaaaaaatga ttcaagatag agatgcagga
     1621 tggaaagatt atataaatat ctgtaaagtt ggaggaacaa aatcattttt agatattgtg
     1681 agtatgggaa atttgtattc tccatttgat gatggctgta ttgagtctat tataggtgat
     1741 gttaaatctt ggtttgatga aataaatgac agcaaattat aaaaaattat gagaatttta
     1801 ttgtataaaa gtgaaaaatt aaaaaatata taaaaagtga ttatttgttg tgaaaatagt
     1861 cttgttttta actaaattga taaaataaac taaaaaagat ttgtacttaa aaaaaaaaga
     1921 aaatacaaga aatatgcaat atcaacattt ggaaaaaaaa gtaaaaatgt atacaaaata
     1981 catataaaaa aactattgca aattttatag aatgtttata tacttaagtc aacagagata
     2041 gaaaaaagct atttatagaa aacaaaggat aaattgctta attttaaatt ttctgaaaaa
     2101 aataataatc cggatgaaga gtatgggaga ctgctttata aaaagcgacc gaagggacaa
     2161 gtttaatctt acttacccaa aagattagat gaaattctca ggtaaaggtg actgtacttg
     2221 gacggggctc tgaaaatgat tttaacaggg aagacaaatt gggagatttg tttttcctgt
     2281 tttttattta tcaagtaata aataaaagtt tgaaagagag taaaaaaatg ataataatta
     2341 cgaataatcc aaaagtaaaa gaagaagttc aaggcagaga agttttgttt aaagacacaa
     2401 cttatattgg aatcttagaa gcaagtagag atttgataca tgaaggttat gaacttttat
     2461 ctcatccact ctatgggagt gtaaagccaa atgaaacacc ttacaggact gttatactaa
     2521 agaaaggaaa tcgtttggat ataaactctt taaccttaat tgaagaagca atcatcactg
     2581 caagtaaatt tcagaataat aaaaagactc caaaatggac agaaagtgtt caagatgatt
     2641 ttagagtaat tgattatgat atattttata atacaataca aagaatgcag tatgaataaa
     2701 aaaaaattgt taaaaagtta ttaaaacagc ctaaatatta aatctaattt aatattaaat
     2761 aaaatgagaa atttaattgg ggaggataag tcaaaatgga aaatgtgtat gacttagtaa
     2821 taattggttc aggaccagca gggcttgcag caggtcttta tggagcaaga gcaaagttaa
     2881 aaactctaat cctagaaaaa gacaagactg gtggacaaat tgtaataaca catgaaattg
     2941 caaattatcc agggtcagtt ccaaatgcaa caggaccaag tttaatagct agaatggttg
     3001 aacagtgtaa agagtttgga gcagaaatgc taagagacaa cattgttgat actgagctag
     3061 atggagatat aaaagtttta aaaggggaaa aggcagaata tagagcaaaa gcagtaataa
     3121 taggaacagg agcaacacca agaaagattg gttgtcctgg agaaaaagaa cttactggta
     3181 aaggggtttc atattgtgca acttgtgatg cagatttctt tgaagatttt gaagtatttg
     3241 ttgtaggtgg aggagacagt gcattagaag aagcaatgta tttaactaaa tttgctagaa
     3301 aagtaacaat tgtacataga agacaaggat ttagatgtgc taagagtgtt gaagaaaaag
     3361 caaaagctaa tccaaagata gagtttttat tagatacagt aattgaagaa atcaaaggtg
     3421 atggaatatt agaatcagtt gtatttaaaa ataaagttac tggagaaact catgaatact
     3481 ttgcagatga agaagatgga acaatgggag tatttgtgtt cgttggatta gatgctcaaa
     3541 ctgatttatt caaaggtaaa gttgatatgg atgaaaaagg ttacataata actgatgaag
     3601 atatgagaac taacatacca ggcgtatttg cagcaggaga ttgtagatct aagactctaa
     3661 gacaggttgt aactgcaact aatgatggag caattgcatc tatagtagct gagaaataca
     3721 ttgatgaaaa atttgggaac tagaatctgt aaattaagaa atagtgtctg taaatttaat
     3781 gaataatatt taaaattaaa attaatattt aaaattaaaa taaaaatttc aagggggaat
     3841 aagaaaatgt tagatttaga taaggctaca tttgaagaag aagttttaaa tgcagaaggt
     3901 tttgtatttg ttgatttttg gagtgaaggt tgtgagcctt gtaaagcttt aatgccagat
     3961 gttcataaat tagctgagac ttatggggat aaaataaaat tctgtaagat ggatacaaca
     4021 aaagctagaa gattagctat aaaacaaaaa gtattaggtc ttccaactat ggctatatac
     4081 aaagatggag aaaaagtaga cgaagttact aaggatgatg ctacagttcc taatatagaa
     4141 aacatgatta aaaaatatct ttaatatgtt gataaattat taacattaaa agaaaaaatc
     4201 tatttttatc tgaagataag
//
LOCUS       DAMJVI010000181         4216 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.187, whole genome shotgun sequence.
ACCESSION   DAMJVI010000181 DAMJVI010000000
VERSION     DAMJVI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.187"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            300..1439
                     /locus_tag="PC545_003563"
     CDS             300..1439
                     /locus_tag="PC545_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDF2458276.1"
                     /translation="MVIKLTIEEYKNKYFTGNKIVLSINSFDNKELYYHETENILNLI
                     NEIDKLTSSKIFKTIEDKNDLALESYYSSVIEGAFSTRKIAKSIIRGKMKPSNKSEYM
                     IYNNHRALEYGLDNLDKLYSHKFIYDLHHILGENCLDSEEYEYRTEKVYVCDSKGEII
                     HTGLEPLKIYDFMSKLIDFMENSKVSNLIKSAIIHFYFVYVHPFSDGNGRTSRALSYL
                     YLIDKGYDTFKDFSISYMISKNRTKYYKAILDVENKGNNLTVFIEFMLKSIIQSINEM
                     RNMHDRKSLESILKEELFENDITLSATEEHILKYICNKDNYSMTLENYIKKNKSRYLK
                     AGIKEIELVDQLMEVFNNLEEIEILSKEKNIYKVNDKYLKMLDID"
     gene            1465..1653
                     /locus_tag="PC545_003564"
     CDS             1465..1653
                     /locus_tag="PC545_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458277.1"
                     /translation="MNNKKKAFLISGAINVISLIIVFSLFHYNIIENSTQNMIITVIL
                     IFIFEMIKIKIIEKYYKL"
     gene            2437..3993
                     /locus_tag="PC545_003565"
                     /pseudo
     CDS             2437..3993
                     /locus_tag="PC545_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
BASE COUNT         1820 a          427 c          675 g         1294 t
ORIGIN      
        1 taaatttatt taatttaatt aaaatattat aacatattag ttatatatta aaatatataa
       61 tcgagatatt gttaagacat tgtcgagata ttataatatg taactaatgt attataaata
      121 atagttgtaa acttcctgtg acatatattt taaatgctta ttcttaaatt tttgcattat
      181 ttattaaaat ttattatgct ttaacgctaa attgagtttg ataattataa aacaaataat
      241 caaaaatagt gtaaaaatta atactttttg ttaatattaa tagataaaga taatctaagg
      301 tggtgattaa actgacaata gaagaataca aaaacaaata ctttactgga aataaaatcg
      361 tactttctat aaatagtttt gataataaag aactatacta tcatgaaact gaaaatatat
      421 taaatttaat aaatgaaata gataaattaa catcaagtaa gatatttaaa acaatagaag
      481 ataaaaatga tttggcacta gagtcttatt attctagtgt aatagaaggt gctttttcaa
      541 ctagaaagat tgcaaaatct ataattagag gaaagatgaa accaagtaat aaaagtgaat
      601 atatgattta caataatcat agagccttag aatatggatt agacaactta gataaattat
      661 atagtcacaa atttatatat gatttgcatc atatattggg cgaaaactgc ttggattcag
      721 aagaatatga gtatagaact gaaaaagtat atgtatgtga ttcaaaaggt gagattatac
      781 atacagggct tgaacctttg aagatatatg actttatgag taaattaata gattttatgg
      841 aaaatagcaa agtaagtaat ttaattaaaa gtgctataat tcatttttac tttgtatatg
      901 ttcatccttt tagtgatggt aatggaagaa catcaagagc cttatcatat ctatatttga
      961 ttgataaagg atatgatact ttcaaggatt tttcaatatc atatatgata tctaaaaaca
     1021 gaactaagta ttataaggct atacttgatg tagaaaacaa aggaaataat ttaactgtat
     1081 ttatagaatt tatgctcaag tctataatac aatctattaa tgagatgcgt aatatgcatg
     1141 atagaaaatc tttagaaagt atattaaaag aagaattgtt tgaaaatgac ataactctat
     1201 cagcaacaga agaacatata ttaaaatata tttgcaataa agataactat tctatgacat
     1261 tagaaaatta tataaaaaag aataaaagta gatacttaaa ggctggaata aaggaaattg
     1321 aattagttga ccagttgatg gaagtgttta ataatcttga agagatagag attttgtcta
     1381 aagaaaaaaa tatatataaa gttaatgata agtacttaaa aatgcttgat atagactaat
     1441 ttagataatc tggagaggga atatatgaat aataagaaaa aggcattttt gatttctggg
     1501 gctataaacg taatttcatt aattattgtt ttttcattgt ttcattataa tattatagaa
     1561 aatagtacac aaaatatgat aataactgta atcttaattt ttatttttga aatgataaaa
     1621 ataaaaatca tagagaaata ctataaacta taaacaagaa atatcttttc taagagcata
     1681 ttgtacaata tgctcttaat ttagttatat agatttaata taaataatgt attttttgta
     1741 gatgatttat gtaagattta tgaccaattt gtgtatgatt tgtgttcttt aatcatgaca
     1801 atattatatt gtaaaaaata aatttaataa aaaatgactt ggaatttata ttctaggtct
     1861 tttttatata aaaatttaat aagtttcaaa tgttggtaac tttgacagta gaaaacgtct
     1921 attgagataa cgaggattct aattgagggg gagagatatt agcacttaaa gtagtatatg
     1981 caacagatat aatctttaca aaagtaattt gaattaaaat gtaaatataa agaataatta
     2041 aaaaaatatc gcaattattt gtttttcata tggataaaat tggttattta atatataatt
     2101 acaagtataa ataataaaat ttatttgtaa aaagagtagc actttaattc ataaaggcta
     2161 ctttttttat acaaaaccct attttttata acaattcatt aacataaaaa tcaaacttac
     2221 cttaaaatat aaatttacaa atttttcaat caataagaaa atttctttat tccttacata
     2281 atgactgcaa tgtaacaaaa taattacaaa ttaattatga aatcattata gttagaaacc
     2341 tctttaaaat tagatattaa gtatccttta gaattagaac gggaacaaag ttaaaaaatt
     2401 aagtaatatc aaatggacaa gggggaagaa aataaaatga aaataccaaa gaagattgct
     2461 gcaatgctta cagttacaat gatagctgga agttcaacag caggcatagc tagtgcacaa
     2521 actgtggcaa caaatttaac agggcaagaa agatatgaaa ctgctgttaa aataagtcaa
     2581 gatggatgga aaaatgctga tgaggtagta atcgtaaacg actcatctat agcagatgct
     2641 ctatctgcaa caccatttgc aaaagcaaaa aatgctccaa tacttttaac aagtaaagat
     2701 aaattaaatg ataagacgaa agcagaaata caaagattaa aagctaaaaa agtatactta
     2761 ataggtggaa cttctgtatt aagtacaaat atagaaaaag aaataaaaga tttaaaaata
     2821 tcttttgaga gaatctctgg agctgagaga tatcaaacat ccttagaatt agcaaagaaa
     2881 ttagatgcta taagtgatgt taagaaaata gctgtagtta atggtgaaaa aggacttgca
     2941 gatgctgtaa gtgtaggagc accagctgca caaaacaata tgccagtaat acttgctgat
     3001 tctaaaaatg gaacagcagt tgcagacaaa tttataaaag atgctggaat aactcaatct
     3061 tatgtagtag gtggagagag ttcaatttct gaagctgtta aaaataaact accaaatagt
     3121 acaagactag gtggaacaga tagaaatgat acaaatgcaa aagtaataaa agaattttat
     3181 aaaaagacag atttaaaaaa tgcttatgta actaaagatg gtatgaataa acaagaccaa
     3241 cttatagatg cactagcagt aggtgtatta ggtgctaaaa atcaatcacc agtagtttta
     3301 gttggaaaga acttatcagc ttcacaaaaa tctctagtta actctaagag ttttgataag
     3361 ataactaaag ttggtgggaa tggtaatgag acagcattta atgaaatgaa gtcattacaa
     3421 gaagtaaaaa cagttgaagc taaaacaatt tctgaattaa aatcagctat agataaagct
     3481 actgctaatg atgttataaa ctttaagcca actagtgagg ttaaagaagc atttacaata
     3541 caaactgata aagcagtaac agtaaactta aatggtacat atacaaagac tgtaactatc
     3601 aacatgccta atggagatgt aaataactat gcaaaagtgg atgatgtagt tatagatgat
     3661 gtaaaagatg gaacttttgt taactatgga aaaataacta acttaaaggt taatgataaa
     3721 aatggagcca agatagaaaa taattctaaa ggtgagattg gtagtttaac agtagcatct
     3781 ggagcaagtc aagtaaaagt gactaatggt ggtaagataa ctacagttac aaataattct
     3841 aaagggacta ctatagataa taaaggtaca ataagtagtg ttaagggaga taattctcca
     3901 acaataagtg gaaatagtcc aagttctaac tcttcaggag gaagttcatc aagtggaggc
     3961 tcttcacatg gaggaggaag ttcttcatca aaaccaagcg ataagtcaaa aacaaagaca
     4021 aaagcagaag aaaaaatagc agaggtaaca aaactagtag gaaaagtaac agcagataat
     4081 ttagaagcat caaaagtaac attaaaagca gcaactgatg caatagaaga agcaaggaaa
     4141 gcagggtgga cagaatcaga agttcaaagc tttaaaggat atggagatat agcaaaagta
     4201 caaagtgaga taactg
//
LOCUS       DAMJVI010000182         4154 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.188, whole genome shotgun sequence.
ACCESSION   DAMJVI010000182 DAMJVI010000000
VERSION     DAMJVI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.188"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(219..602)
                     /locus_tag="PC545_003566"
                     /pseudo
     CDS             complement(219..602)
                     /locus_tag="PC545_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     regulatory      828..925
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            980..1156
                     /locus_tag="PC545_003567"
     CDS             980..1156
                     /locus_tag="PC545_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458278.1"
                     /translation="MFGNKMDKCTHVLTAYISSSYDYCNFLDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            1382..1582
                     /locus_tag="PC545_003568"
     CDS             1382..1582
                     /locus_tag="PC545_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDF2458279.1"
                     /translation="MNNGIVKWFNNEKGFGFISMEGRDDVFAHFSAIQTSGFKSLEEG
                     QQVSFDIVKGARGPQAENITIL"
     gene            complement(1746..1958)
                     /locus_tag="PC545_003569"
     CDS             complement(1746..1958)
                     /locus_tag="PC545_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458280.1"
                     /translation="MVCKIFITLKTILPKTQIIVTTHSPHILQIDSKEEMIVLDMAES
                     DNVYKKELKLGEYGVLGWTNEGLYLH"
     gene            2333..2515
                     /locus_tag="PC545_003570"
     CDS             2333..2515
                     /locus_tag="PC545_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDF2458281.1"
                     /translation="MFKFYNNATLMFLPGTNIKTISERLGHADIKITMNSYSQFLEEM
                     DREAFENLSKILFIVF"
     gene            2586..3431
                     /locus_tag="PC545_003571"
     CDS             2586..3431
                     /locus_tag="PC545_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458282.1"
                     /translation="MNIIKSITLLLTLLIVILMTGCSPRESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISIENLEEFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSKLYYAQACNYMNFDGFPGYSDTTYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQYLNKALYFNKDNKDPKYNLNTI
                     KKHLN"
     gene            complement(3655..3819)
                     /locus_tag="PC545_003572"
     CDS             complement(3655..3819)
                     /locus_tag="PC545_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458283.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
BASE COUNT         1581 a          519 c          476 g         1578 t
ORIGIN      
        1 ataaaagaca gtaatttatc tatagtgtta cttatatttt atatatatgg ctatttttca
       61 acattttaca atatataaca tttatttttt taacttttgt cagtaaaatg tcagcaaaac
      121 actttatcca caatttttat ataagttttc cacaacttat caccaatatt atccacataa
      181 aaaaggtagc acttatatta ttagctacct tttatttatt aatctttata tttccagctt
      241 tttatccaac ttcctatttc tattattttt tcttcagtta tttcttctcc atcatgtaat
      301 tttgaaatta attctgataa aggattgtta tgtaaacctc taaaaactct ttttccctct
      361 attttgagat attctctctc ttttattaat actgtatagt gtacatgcct cccaattctc
      421 tatgatatta aaaaaccttt tttggttaat ctagctaaaa gtataattgt agttgtatgc
      481 ttccaaccat atgttttttc tatttcatct gcaacatctt ttgatattac agttttataa
      541 ccagtatgcc atatgcattt cataattttt aattctgtat ctggtaattt tttatttagc
      601 ataatatatt ccttctttca atttatgtca cttcttcaaa tttatctagc ttaaattata
      661 ataggagctt tttagaacaa aaactatatt taatttttac caggtactct acgtgtttag
      721 tctgttttta agtgtaaaat aatgttcata tctataaatt tatttaactt tcaaacaatc
      781 ctgttgaata ataactaatt ctattgtatt attttagttg tagaataaaa ctaaatcggc
      841 aaaactagag aaatttagtg acgcaaagct atagggacta agacttatat aaatatctta
      901 tgagttatgt cagccagttg ccaaagagat agtgttcttt ttgtttttat gaaagttttt
      961 tagggggata ataatttata tgtttggaaa taaaatggat aaatgtacac atgtattgac
     1021 tgcttatatt agcagttcgt atgattattg taatttttta gatacacagt tagatgattt
     1081 tatattagag tacggagaaa atgtagtaga gtcttgttta caccaagtaa tggtattggt
     1141 aagtaagtat aattagatag acattgattt ctagaaaacc ctttttagtt ttatctacat
     1201 ttatattaat atatagcaat gtaaaacgtt taaatatatt tttaatatta agaataatta
     1261 aaatgttaac taaaaaatat aattaagtag acatttctat taatctatga tacaatagta
     1321 tttgtggtaa tacacaatta aatcaagtat atcttgatat aggttaatag gagtattttt
     1381 aatgaataat ggaatagtga aatggtttaa taatgaaaaa ggattcggtt ttatatcaat
     1441 ggaaggtaga gatgatgtgt ttgctcattt ctcagctata caaacttcag gatttaaatc
     1501 attagaagaa ggtcaacaag taagctttga tatagttaaa ggtgctagag gtcctcaagc
     1561 agaaaatata actatattat aatatataga tttttcggga aagactcttt ttagagtctt
     1621 tttttattgt taatatatgt aagataatca ttggtgaagt aaataaggta tattgagtaa
     1681 tgttcttgat agtttatatg ctaatgaatt tcaaaaaaat aagcactcct ataaaaagag
     1741 tgctcttaat gtaaatataa accttcattg gtccagccta ataccccgta ttctccaagt
     1801 tttaactctt ttttatatac attatcactt tcagccatat ctaacacaat catttcttct
     1861 ttagaatcaa tttgtaaaat atgaggactg tgtgttgtga caattatttg tgtttttggg
     1921 agaattgttt ttaatgtaat aaaaatttta cataccacat agttgatata aaacttaaga
     1981 ctagcagact cataagcaat tagttgcctc tctttacata ccacatagtt gatataaaac
     2041 ctagaggctc tcagagaagt tataagatgt ttagtaactt tacataccac atagttgata
     2101 taaaactcca aaataaactt agcatctcca atgtttacac atatacaatg ctcttaaaat
     2161 tgcagtgaac gcgcaatatt gcaattgata atacttatca tatattctca ataccttata
     2221 tttcaactgc taaacctcaa tttatcacaa atatcgctca ctgcaaaatt tctatatttt
     2281 tattatatca tagcatataa ttaatatttt caattaattt ttacatttaa ttatgtttaa
     2341 attctataat aatgcaactt taatgtttct tcctggaact aatataaaaa ctatttcaga
     2401 aagattaggt catgcagata taaaaataac tatgaatagc tactctcagt ttttagaaga
     2461 gatggacaga gaagcatttg aaaatttgag caaaatacta tttattgtat tttaaagaag
     2521 atgacttatt caatatttga tgctagaata taattaataa aaatttaatt ataggtgatt
     2581 gtattatgaa tattattaaa agcataactt tactcttaac attattaatt gtgatattaa
     2641 tgacaggctg ttccccaaga gaaagtgcta gtgaagttgc aaaaaaatat attgataaga
     2701 tgaactctgg tgactatgaa acagcatata gtttattaag taaagatagt aaaaaaaaaa
     2761 ttagtataga aaatcttgaa gaatttcaaa atattttagt tagtacaaaa agaataatta
     2821 gttataaaat aggtaaagaa aaaatcataa aaaaatataa acatgatgga aaagaatata
     2881 aaaatgttgt taaattagaa gaaactttta atataaaaaa ccttctttat aaaaaagatg
     2941 attctctaaa agtaaataga tattttgtag ttgaaaataa caaatacaag ctattattgt
     3001 atgaaagttt caaaaaagaa tttggtatta atagtatgga ccttgctcaa cttaagctta
     3061 atgattttgg actaaaatac tttaaagaag ttgaccttat actaaaaaaa gctattgcta
     3121 ttaatcctac tgattcaaag ctttattatg cccaagcatg taattatatg aattttgatg
     3181 gatttcctgg ttattcagat acaacatata atgctttaga ttcaattaat aaatctttaa
     3241 agtatttaga taaaaatgat tctgactaca aaaaaaatgc aagtaatata tataacttaa
     3301 aaggagttat tcttatgttt cacaaaagat ttgatgaagc acaacaatat ttaaataaag
     3361 ccttatactt taacaaagat aataaagacc caaaatataa tctaaataca ataaaaaaac
     3421 atctaaacta gatatttaat ttttgaattt ataaaatatt tttttatgta taattcagca
     3481 ctgaatcata atctctacta aactaattac aagtttaaat tataaagact tatttctata
     3541 taaatattac tctataacaa taagtttctt atttaaattt taaaatatta tctaaaaatc
     3601 ataaaaaaga aggtataaag agctaccctc aagctcaata taccttataa atatctaaac
     3661 ttcatttttt actaattttt ctatatcttt gataatatgt tctttagatt tttttccagc
     3721 tatggcatca aaaattgcaa cagccttttt ctctccaaac ttttctgcta catcattaaa
     3781 gtctaaaaat ctaacacttt cttttggttt accaaacata taacacctct catacatttt
     3841 tatatattta ttataacttc tatagactta tttttcacca ataaacgtta gacaaattag
     3901 tccctttatt tttttaaact agctttacct tcaaaattct taacaaaaac agtttattct
     3961 actctataaa taaaaataga caatttaatt tgtaggaaac tgataataga atacagtaaa
     4021 taaaactttg tttaatctat ctgaaaaagt tatctattaa aactgctatg aatttataaa
     4081 gaatattcta gttcataaaa atacaaatat gtaaatataa tcaccttaat attcaattta
     4141 tttaaaataa aaaa
//
LOCUS       DAMJVI010000183         4148 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.189, whole genome shotgun sequence.
ACCESSION   DAMJVI010000183 DAMJVI010000000
VERSION     DAMJVI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.189"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            76..1383
                     /locus_tag="PC545_003573"
     CDS             76..1383
                     /locus_tag="PC545_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458284.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKADIGCVEEIDNEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVVTIDKRY
                     DYRKELKSELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYYNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYVILVEQNKILTDEVFDCNLASKNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1469..1731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1857..2660
                     /locus_tag="PC545_003574"
     CDS             1857..2660
                     /locus_tag="PC545_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458285.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGDTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2698..3309
                     /locus_tag="PC545_003575"
     CDS             2698..3309
                     /locus_tag="PC545_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2458286.1"
                     /translation="MLQGLEIVIAMFCITLIVSIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRVIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3341..4012
                     /locus_tag="PC545_003576"
     CDS             3341..4012
                     /locus_tag="PC545_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2458287.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
BASE COUNT         1645 a          395 c          764 g         1344 t
ORIGIN      
        1 ttatataaat taaattatat aaattaaatt atataatata ttgagtttta cataaaagca
       61 aaggtgggaa taaaaatgaa agaaattgct gatgctataa catcagagta tttaagctat
      121 ggtatcaatg aagatattag tatctatgaa ggtagatttt gcatatattt agataaaaaa
      181 tatagatgta atgggaaaat ttattataaa atgacacctc caatatctat aagttttaaa
      241 gcagacatag gttgtgttga agagatagac aatgaagatg acaatttagc tttagattat
      301 gataatgcta ttcttgaagt tcatggatat aaaattattt ctataacaat aaatacttta
      361 agtgaattta gtgtagaagg ttatatcaat gatgactgca taaaatctaa gaactcctat
      421 gttgaatatg tagattttaa tataataaac ttagataaaa tacctggaaa actcattaaa
      481 tacaatgata aggtatacgc aggtagaatt gaatttgata taaatgatta tgttgtaact
      541 atagacaagc gatatgatta tagaaaagaa ttaaaatcag aattaaagtc taaatctgga
      601 gcaataataa ctcatatagg taggattaga agaaaagatg gtagaatttt tagaacaaat
      661 aatacaataa atcttttaga taggatatca acagctttga gttttatgtg tggaagatat
      721 gtagggtttt gtttggccaa agggtataga agtggtaatg aagtttatag aatatggaat
      781 gaaaatcaaa tatctccttt tagatatgta ccaacatggt cagatacact ttcaaattat
      841 tataatatgg aaaaatatat aagccttatg tgtaaaaagc tagaagattt ttattatggt
      901 tctgctataa agagtgttgt tgattggtac atagaatctc ttggaagtgc taccatggaa
      961 aacaatataa tatctgtaca aattgcactt gaaactttgt catatgtaat tctagtggaa
     1021 caaaataaga tacttacaga tgaagtattt gactgtaatt tggcatctaa aaatataaga
     1081 ctcttacttg atacatgtaa gataccatat ggtaaacatg aattaaatat atttgataat
     1141 ataataaaaa ataaatttga tgatggagtg gatttagtta tttatttaag aaatagcata
     1201 gttcatccaa gtagaaagac tcatagagct gtgttagagg ttgaagatat ttggaatata
     1261 atttcaatag gaacgagata tattgaactt gttttactct ttattttagg ttatagagga
     1321 gaatattcaa acagattagt agaaagatgt tatggtgaag tggaagttgt accttggaac
     1381 tagattttta tatttatttt aaaatatata ttgacatata ttgccttatg ctataaaatc
     1441 aatatcataa taataattca gaataacatt aaatgctaag aaaagaaata gtaaaagtag
     1501 ttaatcttaa acagagagtt tccgattggt gagaggagca atagatttta ctttgaacac
     1561 atctttgagc aggactctga aagagtagaa atactttatt aggagatttc cggaatcctt
     1621 gcacccgtta tagtgctaga gtataataat tttattgtac ttaaagaggt tagtgtcgtg
     1681 aggcattaat gaacagaggt ggaaccacga gagttaactc ccgtcctcta gcagaagcta
     1741 gaggcgggag ttttttaata taatttttta agcgcgcaga gaattatatt gcaatagata
     1801 cactttttaa aaaataatca aaatatttta aaaattaaaa ggcgagggag aatattatga
     1861 aaaatatatt aaaaaaagta ggaatattta caattatgtt aggtttatta ggtggagttg
     1921 taggttgttc aaaaccagat aatgaaaaag ataaggatgc ttcaaaggaa tctaaaaaag
     1981 aagtagttgt agggtttgat aatacgtttg taccaatggg atttttagat gaaaagggag
     2041 atacagtagg atttgacgta gacttagcta aagaaacatt taaaagactt ggaatggaag
     2101 ttaagtttca gcctatagat tggtcaatga aagaaactga attaaatgat tcaaaaactg
     2161 tagatgtatt atggaatgga tattctataa cagacgaaag aaagaaaata gtatcatata
     2221 cagaaccata tttacaaaat aagcagataa tagttactct aagtgactca aaaataaatt
     2281 caaaagctga tttaaaagat aaagaagttg gaacacaaca aggttcaaca gcacttgatg
     2341 cagtggaaaa ggataaagat tttatgaata gcttaaaagg tggagcacca gttctttatg
     2401 atacttatga taaagcactt agagatttag agataggtag aactagtgca gttgttggtg
     2461 atgaagtttt aattagatac tatatgggac aaaagggtga agacaaatac aaggtattaa
     2521 aagacgattt tggattagaa gattatgttg tagcaacttc taaagaaaat ccagaacttt
     2581 gtgagaaaat aaatgaaaca ttaaaagaaa tgaaaaaaga tggaactttt gacaagattt
     2641 atgataagtg gtttaaatag aagataagtt agctgttaag aagggggcac taaaggcatg
     2701 ttacaaggtt tagagatagt tatagcaatg ttttgtataa cattgatagt atcaattcca
     2761 ttggggattg gagttgcatt tttaagatta tcaaagaata aattagtaag tggtattaca
     2821 caatgttata tattgataat gagaggtaca cctttacttt tgcaaatgat agtcatattt
     2881 tacggattac ctcttttagg aattgtattt gacagattta cagctggagt agtagctttc
     2941 ttcttaaatt atgcagcata ttttgcagaa atatttagag gtggaataca gtcaatcgat
     3001 agaggacaat atgaagcttc aaaagtacta ggatttgata agtttactat gtataagaga
     3061 gtaatctttc cacaagtttt taaaagaata ctagcaccta tttcaaatga agtaatcaca
     3121 ttagtaaaag atacatcttt agtatatata ttaggattaa atgatatttt aagaatatct
     3181 caaatagcaa tgaacaggga agcaagtctg ttaccattat ttgaggcagg agcgatatat
     3241 cttatatttg tagctatact tactaaggga tttgaattac ttgaaaaaaa atattcttat
     3301 tatagataaa gttacaatat tataggagga ggttttttat atgttgaaaa tcaaaaactt
     3361 aaataaatca tttaaaaaaa atagagtact taaagatatt tcttttgagt tagaagaagg
     3421 tcagattgga gttttacttg gtaagtctgg tgcaggaaag accacaatat taaggtgtat
     3481 aaatggtctt gaagaatttg atagtggaga gattataata gataatgagg taataaaaaa
     3541 taagagagat atggcaaaaa tacgaggaaa aataggtatg gtttttcaaa atttcaactt
     3601 atttcctcat atgacagtcc ttgaaaatat aattgaatcc ccagttaatg tttttaaagt
     3661 tcctagaaaa gaggctgaag agagggctag agaactttta agattagttg atttagaaga
     3721 taaattaaat tcttatccat ttgaattgtc aggaggacag caacagagag ttgctatagc
     3781 taggtcatgt gcattgatgc caaaagtcct ttgttttgat gaacctactt ctgcacttga
     3841 tattgatact attcaaagag tagtcaatat aatgaacaga cttaaagaca aaggtatgac
     3901 aatattaata attacacatg atgttgtctt ctcaaataat gttgcagata aaattataag
     3961 tataaaagat ggtatagtag aaaatgttca aataaaagaa aaaattgttt agagttttat
     4021 aaatataaac tagggtagaa ttttataata taaataaagg gcaagtaaaa tcaaatatac
     4081 tatgcccttt tgttatctca aaatttaatc ttatatttta tgtttaagtt acattataat
     4141 ttataata
//
LOCUS       DAMJVI010000184         4142 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.190, whole genome shotgun sequence.
ACCESSION   DAMJVI010000184 DAMJVI010000000
VERSION     DAMJVI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.190"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            370..1644
                     /locus_tag="PC545_003577"
     CDS             370..1644
                     /locus_tag="PC545_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDF2458288.1"
                     /translation="MQFMDVSKYIEGANLKVKTKNGETVKRIYFNNSATPLVLKNVID
                     NLNCEIPWLTYINAPGIISEKNTLKYENVRSTILKLIDGDEEKDSVIYVKNATEGINF
                     LAKFFKDENPDKFVITTAMEHMANYLPFKVNFPTKVVGITEKGELDLCQLENIFKNYA
                     GNVSLVTVTGASNVTGITTPIYEIARIAHKYGAKILVDIVQVIQHKPFSMMPHECDEH
                     IDFVVFSAHKCYSPLNGGALVGPKKFFEKFNPVLYGSGSTKFVNDNKILYANVPQRFE
                     AGYPEYFGTLAMGKALNTLNKIGLNRISKYERELFLYAKEEMSKIPNIIIYGDKSNNV
                     IIPNISFNISNMYYKDVAKYLVDNFGIETGAGVVGADIYVQKLLGINPKEAYVRFMRE
                     NPVGLVRISLGMYNTFDEVDSFIHAIKTLAFK"
     gene            complement(1851..2168)
                     /gene="trxA"
                     /locus_tag="PC545_003578"
     CDS             complement(1851..2168)
                     /gene="trxA"
                     /locus_tag="PC545_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDF2458289.1"
                     /translation="MVKIINNNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     NEMVEKANFLKVDIDQSIELAQKFEVSTVPTMLIFKDGKPVDRLIGFMPKENLKNKIE
                     SYM"
     gene            2435..3187
                     /locus_tag="PC545_003579"
     CDS             2435..3187
                     /locus_tag="PC545_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HDF2458290.1"
                     /translation="MDNIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYIPINPNELLKNKKKEFDNSMDNLKNSLQILNKS
                     KSVEYVLNIKGEKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKSLKGLDNIHIHGGNKTTLENKMLYKGIILIVDNDEILTGNLSEGSEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQN"
     gene            3174..4055
                     /locus_tag="PC545_003580"
     CDS             3174..4055
                     /locus_tag="PC545_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDF2458291.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTDASNISDEIKQNVAKEMN
                     LSETAFVSESNIADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLNTNKSIFYQETK
                     AGVLSVELKKINNENIFMMEQNTPEFENLDNCRKELAQMIGVHEDRLLEYPIIKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVHHNILDKTSFIGEQGNFINRPGKVYVNITRENNDITS
                     VKIGGSAYTVLSGEIMY"
BASE COUNT         1622 a          538 c          570 g         1412 t
ORIGIN      
        1 ttttccatta tgttctaatc aatttcttta ctatttttac aactttatta gtcatattca
       61 taattatttc taattagtgt ttaacatttt atgtaaaatt tataaaaaaa tgtatataaa
      121 ttaaaataag tgactatact aaaaatatga tacggataaa caataagttc aacctatctc
      181 ccccaaaatc aagtgagcta cagtttcccc caactgtagc tcattttcgt gtaatttaat
      241 aaacatctca acaagaatta attaacgaaa ccccttataa aaacataaca tatattaaac
      301 aagctcttga ttaaaacttg agctttttta ataaaaaaca gaatagttta ttagataagg
      361 ggttttttta tgcaattcat ggatgtatct aaatacattg aaggtgcaaa tcttaaagtt
      421 aaaactaaaa atggtgaaac tgttaaaaga atatatttta acaactctgc cactccatta
      481 gttttaaaaa acgtaataga taacttaaac tgtgagattc cttggcttac ttatattaat
      541 gctccaggaa taatctcaga aaaaaatact ttaaaatatg aaaatgtacg ctctactatc
      601 ctcaaactta ttgatggaga cgaagaaaag gattctgtta tttatgtcaa aaatgctaca
      661 gaaggaataa acttcttagc aaaatttttt aaagatgaaa atccagataa atttgttatt
      721 accacagcaa tggagcatat ggctaattac ctgcctttta aagttaattt tcctacaaaa
      781 gttgttggaa ttacagaaaa aggagaactt gatttatgtc aattagagaa tatatttaaa
      841 aattatgctg gtaatgtatc tctcgttaca gttacaggag ctagtaatgt cacaggtata
      901 accactccta tatatgaaat agcaagaata gctcataagt atggtgcaaa gatattggtg
      961 gatattgtgc aagtaattca gcataaacct ttttcaatga tgccacatga atgtgatgag
     1021 catatagatt ttgttgtttt tagtgctcat aaatgttatt ctccactaaa tggaggagct
     1081 ctggtaggac ctaaaaaatt ctttgaaaaa tttaatcctg tactatatgg ctcaggttct
     1141 accaaatttg taaatgataa caaaatacta tatgcaaacg taccccaacg ttttgaagca
     1201 ggatacccag agtattttgg aactcttgca atgggaaaag cactgaacac tttaaataaa
     1261 attggtctaa acagaatttc aaaatacgaa agagaattat ttttatatgc taaagaagaa
     1321 atgagtaaaa tacctaatat aataatatat ggagataaat caaataatgt aattatccca
     1381 aatatatctt ttaatataag taatatgtat tacaaagatg tagcaaaata tttagttgat
     1441 aactttggaa tagaaactgg agctggagta gtaggtgcag atatttatgt acaaaaatta
     1501 ttaggtataa atccaaaaga ggcatacgtc agatttatga gggaaaatcc tgtaggtctt
     1561 gttaggataa gcttaggaat gtacaatact tttgatgagg tggatagctt tatacatgct
     1621 attaaaactt tggcttttaa ataagctttt tctattcttt tttatcaata aatagagagt
     1681 agttttgttt tcacaaaaaa gcaaattata tcaacagtca atttcattag caattagaca
     1741 ctcatttgta actatacaaa ctattttaca ctatacaaac aaaaaactca ttacaatgag
     1801 tttttttgtt tatatatgag atttatttta attacttcaa tttactttaa ttacatatat
     1861 gattcaattt tgttttttaa attctcctta ggcataaaac ctattaatct atctacaggt
     1921 tttccatcct taaatattag catagttggt actgtagata cttcaaattt ctgagctaat
     1981 tctatacttt ggtctatgtc caccttcaag aagtttgctt tttcaaccat ctcatttcct
     2041 aaagcttcat atattggtga tagcatctta caaggtccac accaagtcgc aaagaaatct
     2101 acaactacta aaccatcttt attttctact tcatttataa attcattatt atttattatt
     2161 tttaccataa tatacatctc ctttattatc ttcttgatat taatatacta cgccctatat
     2221 aacagttcaa ttaggcactt ttttgtaacc gcctattttt aataggttta atattatgat
     2281 aaatagattt ataagattaa atatttataa gactaaatat ttctaatata ttttattttt
     2341 ttcttaatat agttgacatt ttttccctta aattataaaa tcaaattaat aactacatag
     2401 gtagttacta atcttttatc aaaggagttt ctatatggat aatataatta atgaattaca
     2461 aaatatagga tttactaaat acgaatctca aatatacata tcattattaa aggaaagccc
     2521 tttaacagga tatgaaataa gtaaattatc aggtgtacca caatcaaaag tctatgaaaa
     2581 tataacaaag ctattaaaca ataatataat tatcaatgtt ggaactgacc ctattaagta
     2641 cataccaata aaccctaatg aattattaaa aaataagaaa aaagaatttg acaattctat
     2701 ggataatcta aagaatagct tacagatatt aaataaaagt aaaagtgtag aatatgtttt
     2761 aaacataaaa ggagaaaaaa atatattaaa aaaagcaata gaaatgataa aaaatgcaaa
     2821 acaagaaatt ataatttctt gcccttatga agaaattctt gaattaaaaa aagaactcct
     2881 aattgcttac aaaaaaaata ttaatattga aatactactt ttagaaaaca aaagtctgaa
     2941 aggtcttgat aatattcata ttcatggagg taataaaact acacttgaaa ataaaatgct
     3001 ttacaaagga attattttga ttgtagataa tgatgaaata ttgacaggaa acttatcaga
     3061 aggcagtgag gccatttcaa tttggacaaa aaactctaac attttatata ttggagttca
     3121 atacataaaa catgaaattt atatttcaga acaaataaaa ggaggttata aaaatgaaca
     3181 aaattaagat taaacaagtt gatgctttta caactatacc ttttggtgga aatcctgctg
     3241 gtgtcgttac tgatgcctct aacatttctg atgaaattaa gcagaatgtg gctaaagaaa
     3301 tgaatttatc agaaactgca tttgtttcag agtcaaatat tgcagatttt aaagtacaat
     3361 ttttcacacc aggtttagaa gttgatttat gtggtcatgc aacaataggt actttccatg
     3421 cacttttgga agaagggaag cttaatacta ataagagtat attttatcaa gaaacaaaag
     3481 ctggtgtatt atctgtagag cttaagaaaa taaataatga aaatatattt atgatggaac
     3541 agaatacacc tgaatttgaa aatttagata attgcagaaa agaacttgca caaatgatag
     3601 gtgtgcatga agatagactt cttgaatatc ctattataaa ggtaaatact ggactttggt
     3661 ggcttgtatt tggtctaaaa tctttagatg atttaaaaaa tatctctcca gatttaaata
     3721 aaatcaaaaa atttagtggt gataataata taattggtat aactccattt tgtatagaaa
     3781 ctatagataa aaagtgtgac taccatttaa gatctatagc accatatgtt ggagttacag
     3841 aagaccctgt atgtggaact gggaacgctt gcgtatcatc ttatatagta catcacaata
     3901 ttttagataa aactagtttt attggtgagc aaggtaactt tataaacaga cctggaaagg
     3961 tatatgtaaa tataactaga gaaaacaatg acataacttc tgtaaaaatt ggaggaagtg
     4021 cttatactgt tttaagtggt gaaattatgt attaatccca tacctaataa aaacaaataa
     4081 aaatactagc taactaaagc tagtattttt atttgttatt tttatttaat aatcatactc
     4141 ta
//
LOCUS       DAMJVI010000185         4105 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.191, whole genome shotgun sequence.
ACCESSION   DAMJVI010000185 DAMJVI010000000
VERSION     DAMJVI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.191"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            32..430
                     /locus_tag="PC545_003581"
     CDS             32..430
                     /locus_tag="PC545_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2458292.1"
                     /translation="MIKKIEHVVITNNNIENYINFYKKFGFSSKEMSKNYKLNARNIK
                     INLNIKGYKFLPYVKNLQASRDNLCFKINTNIDEPKKYLEQNEIQIKRGIVECTGVFG
                     NMKSIYLKKTNGNLIELSNHRITESKPWKI"
     gene            418..1356
                     /locus_tag="PC545_003582"
     CDS             418..1356
                     /locus_tag="PC545_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDF2458293.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDTHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFRLMAVAVIIIFSIFLILVFIIPKIEKDNKTRGALIQ
                     GVFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVVILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHINPIILGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMAQQ
                     MDSDSELAGQIVVFTSGFSIITVFLIIFILKQNNLI"
     gene            complement(1740..1964)
                     /gene="secG"
                     /locus_tag="PC545_003583"
     CDS             complement(1740..1964)
                     /gene="secG"
                     /locus_tag="PC545_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDF2458294.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(2557..3849)
                     /gene="eno"
                     /locus_tag="PC545_003584"
     CDS             complement(2557..3849)
                     /gene="eno"
                     /locus_tag="PC545_003584"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDF2458295.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
BASE COUNT         1414 a          657 c          461 g         1573 t
ORIGIN      
        1 aataacttat ataaaatctt aaggagttgc catgataaaa aaaatagaac atgtggtcat
       61 tactaataat aatatagaaa attatatcaa tttttataaa aaatttggtt tttcatctaa
      121 agaaatgagc aaaaattata aacttaatgc tagaaatatt aagataaatc ttaacataaa
      181 aggttataaa tttttacctt atgttaaaaa tttacaagca agtagagata acttatgttt
      241 taaaataaac acaaatattg atgagccaaa aaaatactta gaacaaaatg agattcaaat
      301 taagcgagga attgtagaat gtacaggtgt ctttggtaat atgaaatcta tttatttgaa
      361 aaaaactaat ggaaatttaa ttgaattatc aaatcataga atcacggaga gtaaaccttg
      421 gaaaatttaa tcctatcaat aaatgttgtt cttcctttgt ttctcacaat gtcacttgga
      481 tatgttctaa aaaaattagg tctatttgat actcatactt taaataaaat gaataatgtc
      541 tgcttcaagt ctttcttgcc attattatta ttttttaata tatacaatac agatttagat
      601 ggttctctta attttaggct tatggctgta gctgttataa ttattttttc aatcttctta
      661 atcttagtct ttattattcc taaaattgaa aaagataata agacaagggg tgcattaata
      721 caaggtgtat ttcgtagcaa tttcgttata tttggtattc ctgtgacaat ttcactattt
      781 ggcgataaga gtattggagt cacatcttta ttaattgcag ttattgtacc tatgtttaat
      841 gtattatctg ttgtaattct tgaaattttt agaggtggca aagtaaatat aaaacatatg
      901 attaaaagcg tcttaacaaa tccactcata atcgcctctt ttataggatt gacaatgcta
      961 attttaaata ttaatatacc tacattttta cacaaaacta ttggtgatat ttctaaaata
     1021 gctactcctt tatcacttat aattctaggt ggctctttta gtttttctgc tataaagggg
     1081 catataaacc ccattatatt gggcgtatgt ggaaaactta tacttgtacc tgctatattt
     1141 ttaccaatta gcatttttct tggatttaga aatgtagaat tagcttcact tatgataatg
     1201 ttatctgctc caactgcagt ttcttcattt acaatggcac aacagatgga ttctgacagt
     1261 gaattagctg gacaaattgt agtatttact tctggatttt ctataattac agtattttta
     1321 ataatattta ttttaaagca gaataatctt atttaatgat atttgataac acaaattggt
     1381 attatgagaa acatatataa aatcgattta attgcgtaaa aacatattat tatactattt
     1441 ttactatata atataacttt gtgtatatta tctcattaaa catggtgaaa gtagtgttgc
     1501 tatatatttt ttagtttgaa aaaaatatat aaatacaaac aatatataaa tacaaataat
     1561 aaacgccaac aaatagtcaa tctataaaat aaaaactgga ctaatcatat aaaatcatta
     1621 gtccagcttt tattttacgt actttttatg ttgatgtact tttattaaaa actaaattta
     1681 atttttaaat gtctagcaaa tgcactaatt aaataatggc cagttattca acaatatcac
     1741 tacttcttaa ctataagtaa tgccaatgca tttataaaaa ataatactgc tgttatcttt
     1801 gtaactctag caagaaatgc ttgttttcct tttggtttaa agtaagcttg gcttccttct
     1861 ccaccaaatt ctgaaggtaa agcattctta gaatcttgag gcattatgct aattactagt
     1921 aaaatcccta gtactacttg cacacccatt aaaatgttgc tcattcttgc ccctcctatc
     1981 tgtttattac ttaaatttca ttactcttta gtgtatcaca ataatatagc catttcaaga
     2041 ctattatgct ataaataaca tacaaaaaaa tatattgtta ctataatata ttactatatt
     2101 ctcaatataa aaatgaataa aacttagaag agaaaactat ttaaattaat gcataaatta
     2161 acacttaaaa taatctactc tcattgtata tattataaat agccaccaaa ataaaacaat
     2221 cattttaaaa tattgatata acttttaatt tcaacataat ttaaaataaa taatccacat
     2281 ttagcaataa tactttaatg acaattatga ttaaatcaaa atatgtcttc acttttattt
     2341 tttaataaaa acctcctatg caattagata aaaatgagat agaaacattt attcatattt
     2401 tttctgaatc tatgaataaa attaagtaat aaaatataaa aaaggataag tgaattgaaa
     2461 aatcaattaa cacttatctc ttttatatct tataagatga gcttaaatat cttatattct
     2521 ctcttcatta atctttttta gtaaccattt taataactat ttttttaagt tatagaaaga
     2581 ttttaatcca caatatctag cttgctctcc aaccatttct tctattctta ataattggtt
     2641 gtacttagca actctatcag ttcttgatgg agcaccagtt tttatttgac cagcatttac
     2701 tgcaacagct aaatctgcta tagtagaatc ttcagtttct cctgatctgt gagatataac
     2761 agcagtatat cctgctctct tagccatttc tatagcatct aaagtttcag ttattgtacc
     2821 tatttgatta actttaacta atatagagtt agctactcca ttttcaattc ctttttctaa
     2881 tctctctgtg ttagtaacaa ataagtcgtc accaactaat tgtaatttat ttcctaattt
     2941 ttcagttaat aatttccatc catcccaatc ttcttcgtct aaaccatctt ctatagttat
     3001 tattgggaag ttgtttgacc aatcttcata taaagcaacc atttcagcag cagttaactc
     3061 ttttccttct cctgctaaaa cgtatttttt agtttctttg ttgtacattt ctgtagcagc
     3121 aacgtcaaga cctaacataa cgtcttcacc tggcttatat ccagcttttg ttatagcttc
     3181 aactattaat tctaaagctt ctctgtttga acctaagttt ggagcaaatc caccttcgtc
     3241 acctacacca caagctaatc ctttttctcc taaaactttc tttaacgaat ggaatacttc
     3301 tgctcccatt cttaaacctt ctttgaaaca acaagctcct actggtaata tcataaattc
     3361 ttgaacatca acgttgttat cagcatgctc tccaccgttt aatatgttca tcattggaac
     3421 aggtaattgt ttagcattta ctccacctag gtattggaat aatggtaaac ctatttcatc
     3481 agcagctgct ctagctactg ccattgaaac acctaatata gcattagctc ctaatttacc
     3541 tttgtttgga gttccatcta attctatcat gattgcatct atagctggtt ggtcaaatgc
     3601 atccattcct tctatttcag gtgctattat ttcatttacg ttagcaacag ctgtttctac
     3661 accttttcct aagtatcttc ccttatctcc atctcttaat tcaacagctt caaaagctcc
     3721 tgtagatgct cctgatggaa ctattgctct tcccattgct ccatcttcta atactacttc
     3781 aacttctaca gttggatttc ctcttgagtc taatacttct ctagcatata ctaattcaat
     3841 aactgacatt tgtatatctc ccttcgattt tctattagtt aaattttcat ttactgtaaa
     3901 catccgtcta ctattttaat ctactttcta tattatagta cataaagttg cttttgaaaa
     3961 ttaggtacaa atttatttga tgggtaaatt tgtaaaagtt cctattttta ttttattttt
     4021 tataatattt taatttatat aatataaaaa aacttgtcta aaatccaaaa ccaatcttaa
     4081 atacacaaat attataacta gattt
//
LOCUS       DAMJVI010000186         3991 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.192, whole genome shotgun sequence.
ACCESSION   DAMJVI010000186 DAMJVI010000000
VERSION     DAMJVI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.192"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            40..669
                     /locus_tag="PC545_003585"
     CDS             40..669
                     /locus_tag="PC545_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HDF2458296.1"
                     /translation="MELNELFNFKYLIIAVIFIISVTMLILISHINILIIADIKNKDI
                     CLKLNIKYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSIYRLLKKI
                     KIHELYSNINFGTGNIGLTSSVYVLINTLYGNLFNMIDAEKMYLNVNPDFTKDYVLGN
                     IRIHIRPRIKALFNIIIMINKIMNKNKGNKEGDSNESNRFDTESYGNNS"
     gene            623..1051
                     /gene="ytfJ"
                     /locus_tag="PC545_003586"
     CDS             623..1051
                     /gene="ytfJ"
                     /locus_tag="PC545_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HDF2458297.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            1299..1739
                     /locus_tag="PC545_003587"
     CDS             1299..1739
                     /locus_tag="PC545_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2458298.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1755..3206
                     /locus_tag="PC545_003588"
     CDS             1755..3206
                     /locus_tag="PC545_003588"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDF2458299.1"
                     /translation="MNIKYSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLIENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEAYKSS
                     DVNVLNLNLNSDEIGSFVEGILLGNYKFDKYKTKIKDFERKEVDTITIFTEKEVECQI
                     EKAKILANSTIIARNLVNEPSNVIYPKTLAVETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIISSMAKKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKAKGATGEPVRTLYYLAEI"
     gene            complement(3297..3965)
                     /locus_tag="PC545_003589"
     CDS             complement(3297..3965)
                     /locus_tag="PC545_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HDF2458300.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSKNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFRVNRYALRDNLAI
                     CFIYFSFIFLLNTFTNSRYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
BASE COUNT         1665 a          360 c          679 g         1287 t
ORIGIN      
        1 attaagtata aatagtaatt ttttgatgat aatataaaca tggagcttaa tgaattattt
       61 aattttaagt atttaataat agctgtaatt tttattataa gtgtaactat gttgatttta
      121 atatctcata taaacatctt aataatagct gatataaaaa acaaagatat ttgtttaaag
      181 cttaatataa aatacatgtt taatttaata aatataaata gacaacttta tccagctgaa
      241 aatagtaaaa ataatgataa aaaagagggg atgaagaata atatagattc aagtatatta
      301 ttagctgatg accttttgag catttataga ttattaaaaa agataaaaat acatgaactt
      361 tattcaaata ttaattttgg aactggaaat attggcttaa catcttctgt atatgtattg
      421 ataaatactt tgtatggaaa tttatttaat atgattgatg cagaaaaaat gtatttaaat
      481 gtaaatccag attttacaaa agattatgtt ttaggaaata taaggataca tataaggcca
      541 agaataaaag ctttatttaa tattataata atgattaata aaattatgaa taaaaataaa
      601 ggaaataagg aaggtgatag taatgagagc aacaggttcg atacagagtc ttatggaaac
      661 aactcttgaa acaattaaag gttctataga tgcaaatact attataggag accctataaa
      721 aacagatact acagtggttg tacctatatc aaaggttaca attggctttg gtataggagg
      781 aggagaatac tcaaaaggat atgatgataa agatagggaa attgaattaa aaaatgaaaa
      841 atcagatacg aattttgctg gaggtagcgc aggggctata tctgtacaac cagtagcatt
      901 tgtagtagtt gaaagtggag agactagaat aatgagttta gacagtaata tcaatctagt
      961 ggataatata ttatctataa ctcctagagt attggagaaa attcaaaata tatcacaaag
     1021 taataagaat caagataaaa acaatatgta agaataaaat atttttaaat atgtatatag
     1081 ttttaggtaa agtagtgggg ttagtataaa tcccactatt ttgttgtgta ctttaaacat
     1141 ttatatctaa taagatttta gtttaaaaca atttaaatag ataataaagt tgatatatag
     1201 tataattata aaaagaaaag aagtattata ttatagatat ataaacgcat ttgagatata
     1261 agagggagtt ataaatcatt agttttgagg tgaatgttat gagcgataaa gaattatcta
     1321 ttttgttgtt agaaagtatg aataattttc ataatttaat aaagattatt aataacgaaa
     1381 gatataaaaa agataaggtt cttacagaaa gacagttctt tgccttagta aaaataagaa
     1441 aacatgataa aatagagctt aaaaatctaa gtagagattt gcatgtatct acttctagtc
     1501 tgtgtatact acttaataaa ttggttgagc aagagtatgt atatagggaa gaagatagta
     1561 gagatagacg aaatactttt tatggaataa ctaaaaatgg agaaaagata ttggataatg
     1621 aaatactaaa gtttgtatct ataataagtg ataaaatgga ttgcctagat atagataata
     1681 aagataaatt atttacttct ttggaagagt ctaaaaatat aatagaacaa ttattttaaa
     1741 aaggagaatt tattatgaat ataaagtatt ctaaagaaat attaaaaagt ttagaaaatg
     1801 ctataacagt agtagctcta tttgagggag aagatgtttc tacgacattt gataaaataa
     1861 atatgataag cagaaatttt aattctaaag aaaatagtat agatactgta acattgattg
     1921 aaaataacaa tttggtaaat gtttgtcttg ttggttttgg taagagagag ttattgaata
     1981 gagaaaaaat tagaatcata ggtggtaatt tgtgtaaaaa attaaaaaat atattgaaaa
     2041 atgaagcata taagagtagt gatgttaatg tactgaatct taatttaaat agtgatgaaa
     2101 taggttcttt tgtagaaggg atattacttg gaaattataa gtttgataag tataagacaa
     2161 aaattaagga ttttgaaaga aaagaagttg atactataac tatatttact gaaaaagaag
     2221 ttgagtgtca aatagaaaag gctaaaatat tagctaattc aactataata gctagaaatc
     2281 tagttaatga accatcaaac gtaatatatc caaaaacatt agcagtagaa acagttaaac
     2341 ttggtgcaga gtttgggttt aatgttgatg tttatgaaga agaaaaaata aaggcattgg
     2401 gaatggatgc ttttttcgct gtttcaagag gctcaattaa taaaccaaga ttcatagtaa
     2461 tgagatactt tggagataaa gaaagtacag atatattagg tttagtaggt aaaggattga
     2521 cttatgatac tggtggatat tctttaaaat caaatgcttc tatgctagat atgaagacag
     2581 atatggctgg tgctgcgagt gttataggtg ctatgtgtgc tatatctcaa agtaaattaa
     2641 agaaaaatgt tatagcagta gtggctgcat gtgagaatgc tttatcagga ggttcataca
     2701 aacctggaga tataattagt tctatggcaa agaaaactat tgaagtttta aatacggatg
     2761 cagaaggtag acttacatta gctgatgcaa tatactacat tataaacaat gaaaaagtaa
     2821 caaaggtggt tgatgtagct acattaactg gtgctgcttt aacattgtta ggtaatgttg
     2881 caactcctat agtaacaaat aatgatgatt tttattgtga attagaaaaa gctgccactt
     2941 tatcagggga aagggtatgg aagatgccaa tttatgatga atttaaagat atgataaaag
     3001 gagaagaagc agaccttaaa aacactggtg gtaaaaatgc tggatgtata acagcaggag
     3061 cttttattgg tgaatttgtt gggaatactc cttggataca tatggatata gcggggacaa
     3121 gtacatcatc aaaatcaatt ggatacaaag caaagggggc tacaggagaa cctgttagaa
     3181 cattgtacta tttggcagaa atctaaaaaa caattataaa atataattta taaagccacg
     3241 tggatatttt tgtaatttaa gattatctat gtggctttat tttttattta aataaattat
     3301 ttactctttt tctttttttc aagtataaaa gtatctctat taaataaaag agcaggtatg
     3361 tacataatac tagtaacaaa aataaacgta aaaatagcag ttgtgctatg acttaaaaaa
     3421 ccataatagt acctagagtt ggtgaatgtg tttagtaaaa atatgaatga aaaataaata
     3481 aaacatatag ctaagttatc tctaagtgca tatctgttaa ctctaaattt tctaacatca
     3541 accatatata tatctgcata aattatcaag cacttagaga agataaagat aaaatatatt
     3601 aagctaggaa attcatagcc caagttttta gaaaaaatga gttctccaac tgaacaaact
     3661 aaactccaag aaaaaaatat attaaaaaga tgatactgct taaaaattag tatggctata
     3721 cacagatata tacaaatata attgattcca attggtaaag agtttagagt actataatta
     3781 cctgaagaaa taagtaataa ttgctctaga gccataatta ttacaagtaa tgtacaaatt
     3841 atcttctcaa ctgtatagct ataaggtaaa atatttttgg ttagttttgg acaatagtat
     3901 aaaaataatc caaatataaa gagacttaca aattgttcac tagaaaaaag aaaaatttta
     3961 aacatataac attctctcct ataataaatt t
//
LOCUS       DAMJVI010000187         3927 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.193, whole genome shotgun sequence.
ACCESSION   DAMJVI010000187 DAMJVI010000000
VERSION     DAMJVI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.193"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            134..1789
                     /gene="ilvD"
                     /locus_tag="PC545_003590"
     CDS             134..1789
                     /gene="ilvD"
                     /locus_tag="PC545_003590"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDF2458301.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSAM
                     EGVGACKDGTVSEEELEALAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLNFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGKTIGENIRNCEIENEEVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFGKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIENRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2133..2765
                     /locus_tag="PC545_003591"
     CDS             2133..2765
                     /locus_tag="PC545_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2458302.1"
                     /translation="MNREEKSKNSKEKIIQSAFSLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLEHLVADDTLTANEKIIELVVHSANDYTRRK
                     MVHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPEELAEMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDEYGIQKFKDLFRQL"
     gene            2788..3600
                     /locus_tag="PC545_003592"
     CDS             2788..3600
                     /locus_tag="PC545_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2458303.1"
                     /translation="MFYNAKSCQMMIGSNTVNYIEFGSGKKTLIILPGLGDGLFPLHG
                     KIQAIAFAFRYKQFAKDYKVYVFSRKNQITEKYSTRDMAKDQADIMKKLGIMKAEVMG
                     VSQGGMIAQYLAIDYPELVEKLVLAVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDT
                     AKKSYSERYLKKYQLFIPFLGKVGKPKDFKRFIIQATSCIEHNAFSELNKITCPTLII
                     GGANDKIVGNNASFHLAEKIKKSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1521 a          444 c          737 g         1225 t
ORIGIN      
        1 tattgataaa aaatcgtaat aaattttcat tagtatatgc taatattttt ttacaatttt
       61 cgtaattaat taatagaaaa atatattata ataatattat gaaaatttaa aaaagtaaga
      121 aagaggtaat attatgagaa gtgatataaa aaaaggaata gaaggagctc caaagagagc
      181 gttaatgtat ggaatgggtc ttactaaaga agaaattgaa agaccattaa taggtatagt
      241 aaatgcacaa aatgaggtga ttcctggtca tctgcatctt gatgaaatag cagaagctgc
      301 aaaaaatgga gtaagaatga gtggtggttt gccattagaa tttcctgcta taggggtatg
      361 tgatggtata gctatggggc atgttggaat gaattattcc ctggcttcaa gagaattaat
      421 agcagattca atagaagcta tggctatggc acatggtttt gatgctttgg tacttatacc
      481 taattgtgac aagatagtgc caggtatgtt aatggctgct gcaagactta atataccaag
      541 tattgttgtg agtggagggc ctatgcttcc aggtaaaaaa aatggaaaag tatatgattt
      601 taactctgca atggagggtg ttggagcatg taaagatgga acagtaagtg aagaagaatt
      661 agaagcgtta gccatgaact cttgtcctgg atgtggttca tgttctggtc ttttcacagc
      721 aaatagcatg aattgtttaa cagaagcttt aggcatgggt ataccatata atggtactgc
      781 tgcgtcacat tctggagaaa gaaaaaggat agcaaaatat gcaggtatgt atgttatgga
      841 gttacttaag aacgacataa aacctagaga tattttaaca atagatgctt ttaaaaatgc
      901 tatagctgtg gatatggcaa tggctggttc tacaaataca gtacttcact tacctgcaat
      961 agcttatgaa tcaggaatag agcttaactt aaattttttt gatgaaataa gtgaaaaaac
     1021 tccttgttta acaaaattaa gtccaagtgg aaaacatcat attgaagatt tacatatggc
     1081 aggaggaata ccagctataa tgaacgaact ttcaaagata aatggaataa atttagattg
     1141 caaaaccgta acaggcaaga ctatagggga aaatataaga aattgtgaaa tagaaaatga
     1201 agaagtaata catacattaa agaatccata tagtaaccaa ggtgggcttg caatattgaa
     1261 aggaaatctt gctctaaatg gagctgttgt aaaaaagtca gcagttgcag aagaaatgtt
     1321 agttcatgaa ggaccagcaa gagtttttaa ttcagaagaa gaagctgtaa atgctatttt
     1381 tggtaaaaaa ataaacaaag gtgatgttat agttataaga tatgaaggtc caaagggtgg
     1441 tccaggaatg aaagaaatgc tatctcctac atcagcagtt gcaggaatgg gacttgataa
     1501 gcatgtagca cttcttactg atggtcgttt ttcaggagca actagaggag catctatagg
     1561 ccatatttct ccagaagcta tggaaggtgg tttaatcggg ttagttgaag aaggagatat
     1621 aatttctata aacataccag acaaaaaatt agagttaaaa gtagatgaag ttgaaataga
     1681 aaatagaaaa ttaaaattta aacctttaga accgaaaata aagcatggat acttaagtag
     1741 atatgctaaa ctggtaacat cagcaaatac aggagcagtt ttaaaatagt agactatatt
     1801 tatgtaaatt agaaaaagtt aagaaaatat taattggata tcttaggtgt tatagcacta
     1861 atgaggtatc ctttttattt tgtatgaaaa acacagcaaa atcttgatta tctttgaaaa
     1921 caatacatct ctattataag gaatacgagt acagcataga gatgtatctt ttagaccttg
     1981 ttgaggtaat acaacttttt aaaagtttga tgtggaatgc ttttcgttcc atctgaacta
     2041 tgaagaaatt gaaagtcagt taattcttga cataataaat attaaaatgt ataataaaaa
     2101 acaaacagac agtctgtttt taggagggaa atatgaatag agaagaaaaa agtaaaaata
     2161 gtaaagaaaa aattatccaa tcagcatttt cactattttc gtctaaagga tatgattcaa
     2221 catctacaca agatattatc aatttatctg gtctatctag aggtgcaatg tatcatcact
     2281 ttaaaactaa agaagatata ctgagaagtg tcacaaaaga actttactca caaatgaata
     2341 attttttaga gcatcttgtt gctgatgaca cccttacagc aaatgaaaaa ataatagaat
     2401 tggttgttca tagtgcgaat gattacacac gtagaaaaat ggtacattgt agctggttag
     2461 aaaaaatccc attcgcttta atagaggaag ttcgtaatct caacaatgta gttgcaccca
     2521 atattgctaa gataattaaa caaggtgttg aaaataaaga attttcttgt gaatatccag
     2581 aggaattagc tgaaatgctc gtttttagta ttgatatttt acttgaccct gtattattta
     2641 aacgtgaata cagtgaagta tgtaataggt tggatttttt attattcatg ttaaaaaaaa
     2701 tggatattcc tctaattgat gagtatggta ttcaaaaatt taaagattta tttagacaat
     2761 tataatagga aaagcgagga atagactatg ttttataatg caaaaagttg tcaaatgatg
     2821 ataggaagta atacagtgaa ttatattgaa tttggtagtg gtaagaagac actaattatt
     2881 ttaccaggat taggagatgg tttatttcct ttacatggaa aaatacaggc gattgctttt
     2941 gcttttagat ataagcaatt tgcaaaagat tataaggttt atgtatttag tagaaaaaat
     3001 caaattacag aaaaatattc cacaagggat atggctaaag accaagcaga tataatgaaa
     3061 aaacttggaa ttatgaaagc agaagtaatg ggtgtttctc aaggtggaat gatagctcaa
     3121 tatctggcaa ttgattatcc tgagttagtt gagaaacttg tgttggcagt tacttcttca
     3181 aagcaaaatg atactatcca aaatgttatt tgtagttgga ttgatatggc gaagaagcag
     3241 aattacaatg atttaatgat tgacacagca aagaaatcct attctgaaag atatttaaaa
     3301 aaataccaat tatttattcc ttttttagga aaagtaggta aaccaaagga ttttaaacga
     3361 tttattattc aagcgacctc ttgcatagaa cataatgcct tttccgaatt aaataaaata
     3421 acatgtccaa ccttaattat aggaggtgct aatgataaaa ttgttggaaa taatgcttct
     3481 tttcatttag cagaaaagat taaaaaaagc gaaatattta tctatgaggg attgggacac
     3541 gctacatatg aagaagcaca ggattttaat gaaagagttt tagagttttt aaataagtaa
     3601 acacctttgt taaaaatata catatgaaga ttggaaattt aatgttaaaa atagaaacat
     3661 gaaaatatgc ttaactggta tttttactat tcataaccaa tttttgatac attatctact
     3721 ataaatacaa atatagcttc aatgtgatta tatattagtg tattggtaaa gcacttatac
     3781 aaacagagga attttgtaaa ttcatattat acccacattt gttaacttat gaaaatataa
     3841 tcaaaatttt tatgagattt ttatataaaa aatataatgt ttgtatacaa aaatattgaa
     3901 gatattttag taagttttgt atataag
//
LOCUS       DAMJVI010000188         3888 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.194, whole genome shotgun sequence.
ACCESSION   DAMJVI010000188 DAMJVI010000000
VERSION     DAMJVI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.194"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            78..1031
                     /locus_tag="PC545_003593"
     CDS             78..1031
                     /locus_tag="PC545_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2458304.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1021..1977
                     /locus_tag="PC545_003594"
     CDS             1021..1977
                     /locus_tag="PC545_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2458305.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1974..2729
                     /locus_tag="PC545_003595"
     CDS             1974..2729
                     /locus_tag="PC545_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2458306.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            2821..3786
                     /locus_tag="PC545_003596"
     CDS             2821..3786
                     /locus_tag="PC545_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458307.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT         1566 a          369 c          658 g         1295 t
ORIGIN      
        1 ttattattga taaactataa aagtgaaaat gataataatt ttcattttaa ttaataaaag
       61 atttttaagg agggagaatg aagaaaaggt atttagtcat aggaataata attttgtctt
      121 atttttcttt atttatagga gctgaagata taaatataat gcatatattt gccaaaaatc
      181 agcataaact tatgatattt atcatgagta gaataccaag attaataagt atattgattg
      241 ctggtgtagg aatgagcata gcaggtctta taatgcaaca gataagtaag aataaatttg
      301 tatcacctac aacaggtgct acaatagatg cagcacaatt tgggatagta atatgtatgc
      361 ttttagtacc aactgcctct atttttacaa agacaataat agcatttgta ttttctcttg
      421 tagggacttt tacgtttatg aaaataatag ggaagttaca atttaaaaat ataatctttg
      481 taccattagt aggtataatg tttggaaata taataggttc tatgacggat tttatcgcgt
      541 ataaatacga tttgagtcaa aatgttagtt cttggatgca aggtgacttt tctatgatac
      601 ttaaaggaaa ttatgagata ttatatatca caatacccct tataatatta gcatatattt
      661 atgcaaataa atttactgtt gttggtatgg gtatggattt tgcgacaaac ttaggtctta
      721 gttacaaaag aattgtcaat ataggtctga taatagtagc acttgttaca gtatgtgtag
      781 tcgttacagc tggtaatatc ccttttatag gtctaatagt tcccaatatc gtatccttgt
      841 atatgggtga taatataaga gcaagcatat ggtatactgg tttacttgga gcgatatttg
      901 tacttatatg tgatattttt ggaagaatta ttatttatcc atatgaaata tctatagggc
      961 ttacagttgg agttatagga agtatactat tcctttattt aatattaagg aggaatgtaa
     1021 atgaagctta gtctcaatca aaaaatttac tttgtaagta tattactatt catatcaata
     1081 gcacttttta taatttatgg aatagacatg aatcatttag aatatgcatt atcacaaaga
     1141 ataccaaaaa tatttgctat ggtattaggt ggaggatgta tagcatttac tactgtagtg
     1201 tttcaaacaa ttacaaacaa tcaaatatta acaccaagtg tactaggttt agattcattg
     1261 tatgtaatga ttcaaactat aatagtattc atatttggtt caagcagtag tctgattata
     1321 aatgaaaatt ataattttat tattaatgta gctatgatga ttggagcttc attgttatta
     1381 tataagacgt tatttgaaaa aaataaaaat aatattttct ttttaatcct agtaggaatg
     1441 atatttggaa ccttatttaa aagtgcaaca acatttattc aggtaatgat agaccctaat
     1501 gaatttttag ctttacaaac tagtataatg gcaagtttaa acaatataaa cactaatgta
     1561 ttgttaatag catttataat aattattgcc ataatacctt ttatatatga tgaaataaag
     1621 tatttagatg tattgtcgtt aggtaaagaa caagcaatta atttaggtgt agattttgat
     1681 aaagcagtta aaaaaatgat aatattaata gcaattttag tctcaatatc aacagcactt
     1741 attggaccta tgacattttt aggactttta cttgctaata taacaagaga aatttttaaa
     1801 acatataagc atacatactt gatttcgggt tctatgttga tagggatgat tacattgata
     1861 ataggtcaat tttttataca acatgtattt aagtttgata caactttaag tgtagtaata
     1921 aattttatag gaggaattta ctttattcaa ttactattaa agggggcaaa tagatgatag
     1981 agataaaaaa tatatttaaa agatataaga ataaaaatgt tgtagatgat gtctctttta
     2041 gtatagaaaa gggaaagata acatctttta taggaccaaa tggagctgga aaaagtacag
     2101 tactatcaat agttacaaga cttataggag gtgatggtgg agaagtaata atagaaggaa
     2161 aaagcttgac taattatagc aataaagaat tagcaaaaaa aatagctatt cttaaacaat
     2221 ctaacaatat aactttaaag cttacaataa gagaattagt tggatttgga agatttccat
     2281 atagtgaagg taatctaact aaagaggatg aaaattatat tgatgaagca atagaatata
     2341 tgaagttgac tgatatacaa cataagtatt tagatgaatt gagtggaggt caaagacaaa
     2401 gagcttatat agcaatggta atagctcaag atacagagta catattactt gatgaaccac
     2461 tgaacaattt ggatatgaac cattccgtac agatgatgaa ggtacttaga agtctttgtg
     2521 atgagttaga taagactata gtattagtaa tgcatgatat aaattttgca tcatgttatt
     2581 cagataatat agttgcatta aaaaatggaa aagttaaaaa agtaggtcgt acagatgaga
     2641 tagtaaatga aaaagtttta gaagatattt atgagatgaa ttttaacatt aagaatataa
     2701 atggaaatcg aatctgtatt tatttttaaa atattgtata tgaaaataaa cttgctatat
     2761 gattttttat aaagttttta aattaaaata aagattaata ggagagagga aaatattgat
     2821 atgaataaaa aagcagcaat agtagcagca gtagctatta taggattagt gacagtgttt
     2881 gcattaggtg gaagtaaaaa gaatgaatca aaaacatcag aaaattcaaa taatactatg
     2941 aagataactc ataacttggg agaaacagat gtaaaattaa atcctaaaaa agtagtggta
     3001 tttgattatt cggctttaga tacaatggat gcattaggtg tagctgagaa tttagtagga
     3061 cttccaaaag cgagtttacc agcatcctta gaaaaatata aggatgacaa gtatgcagat
     3121 ttaggaggat taaaagaacc agatttagag ggtataaagt cggcaaatcc agatttaatt
     3181 ataataaatg gaagacaaga agatttttat gagcaattgt caaaaatagc accaacaata
     3241 agtacaagta aagatgataa gaagtactta gaatctgtta aaaataatat agacaaaata
     3301 gcaaaaatat ttggagtaga agaaaaagca aatcaagaat ttagtaaaat tgaaaagaaa
     3361 atagaaactt tgaataaaaa agtaacagat aaaaatttaa atgctttaac tataatggta
     3421 aatgaaggta atttaagtgt atttggtgaa gaatcaagat ttagtatatt atacaatagt
     3481 tttggatttg aaaataaaga taaaaatata aaagaatcaa gtcatggaca aaatattact
     3541 tttgaatata tagctaaaca aaatccagaa gttatgttcg ttatagatag aggaatagct
     3601 actggcagtg atgtaaaaga aagctcaact gcaaaatctg ttttgaacaa tgatataata
     3661 aaatctatgg atgcatataa gaatgataat ataatatatt tagattcacc aacatggtat
     3721 gtaaatgatg gtggtctaac atctttaaat aaaatgatag atgatgcttc aaaagcggta
     3781 aattaataat ccgatataat caaaatatat tttttataaa atgattatca aataaactag
     3841 gagtttttaa gaactcctag tttatttttt gcataaaaaa gatatagt
//
LOCUS       DAMJVI010000189         3872 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.195, whole genome shotgun sequence.
ACCESSION   DAMJVI010000189 DAMJVI010000000
VERSION     DAMJVI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.195"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(175..570)
                     /locus_tag="PC545_003597"
     CDS             complement(175..570)
                     /locus_tag="PC545_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDF2458308.1"
                     /translation="MKALEFLNKARVYYLATVDENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
     gene            711..1103
                     /locus_tag="PC545_003598"
     CDS             711..1103
                     /locus_tag="PC545_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458309.1"
                     /translation="MSINTEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESTGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNTCGLSCKECRKIK"
     gene            complement(1203..1619)
                     /locus_tag="PC545_003599"
     CDS             complement(1203..1619)
                     /locus_tag="PC545_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2458310.1"
                     /translation="MNWQDAFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVSESNRVEVDLFINTCCDYIK
                     KIYNEVNFFNGSKWLMIQVDSLLVLNDMLELIKFRA"
     gene            complement(1634..2545)
                     /locus_tag="PC545_003600"
     CDS             complement(1634..2545)
                     /locus_tag="PC545_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDF2458311.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLARASEYLAEHKNRILDVALNFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVNRKTLSNPRTFVGIEIEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VESGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTTD
                     FAIEMYYPTTPEAAYLEHWIVPVPIEQ"
     gene            complement(2727..3749)
                     /gene="splB"
                     /locus_tag="PC545_003601"
     CDS             complement(2727..3749)
                     /gene="splB"
                     /locus_tag="PC545_003601"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HDF2458312.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKKIYNKFRDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GREEKGRFRFVTKYSDVDSLLDAEHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIISIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFLKEINGVFKNS
                     LIKYII"
BASE COUNT         1295 a          661 c          437 g         1479 t
ORIGIN      
        1 tctagtatgt aattattcaa aatagtatat cattattgaa atacacatct aataactaaa
       61 aatttttaca tattcattat ttaaaaatct ttttattgca tttaaattaa atctatcaac
      121 atttcagtaa taacgttttc ttaattttca ctatggatgt cgattcaatt ataattaaag
      181 ctctacatca atctttttac cactcattga acaacaacta acttttgcct tatcaataga
      241 gaatatttca aacttaccat ctcctacaga gtacatctga cctaaacttg gcattatttc
      301 aagtgcctta gcttgtgttt cttttgatgt aacaaatact gcttcaccta aaatacgtag
      361 agtattaagg ttttggtcta tacaacaaag ttcaaccttt ggattaaaaa taagttgctt
      421 aaacatatcc ttttgattag atgtgcaaaa tgtaagttta ccattgtatt ccataacaaa
      481 tcctaaagga cgcacatgtg cttgattgtt ctcatcaact gttgctaaat aataaactcg
      541 tgccttgttc aaaaattcta atgctttcat aattcttaca ccccttttaa atacttgtat
      601 taaggattat cctttgatat aattttaata taaaatacag aaaaaacaag tatgcagttt
      661 tttctgataa agtataaaaa agatactact ataactaagg agtaattaaa atgagtataa
      721 atactgaaaa aaatataact aaaaacgttg aagaattaac ctgtccaatc cgttatgcat
      781 tagacattgt cggtggtaag tggaaattac ctattatatg tatgttagca gttgaaaatc
      841 caattagata tagtagcata aaaagaaaat tggatggcat tacaaatact atgctagctc
      901 aatcattaaa agatttagag tctactggta ttgttcatcg taaacaatat aatgaaattc
      961 ctccaaaagt tgaatataca ttaacaagta aaggtaaaag tattgtgcct attttacaac
     1021 aatttgctaa ttggggtgct actaatatgc aagaaaaaaa cacatgtgga cttagttgca
     1081 aagaatgtag aaagataaag taaatttaaa tttactaaat tattcatatt atagactata
     1141 tattaattta tctgattgaa tataaaaaca ggatgactta tatattcatc ctgttctata
     1201 tgttaagctc taaatttaat tagttcaagc atatcgttta ataccaataa actatctact
     1261 tgaatcatta gccatttact tccattaaaa aagtttacct cattatatat ttttttgata
     1321 taatcacaac aagtattaat aaataaatca acctctactc tattgctttc acttacaata
     1381 accaaagctt taaaataacc ttcttgtgga tataaagtac atagtgattt acctcttttt
     1441 ttatatttta cattccaacc tctttgcatt gaacatttac tgtattcaag cttaggttct
     1501 acattataag tagaaaccaa agttttattt aacttgtccc ataatggatt attaataaat
     1561 tcattaattt gctcaaatgt aggtttagta tcttgaaaaa acgcatcttg ccaattcata
     1621 ttcatcatcc tttctattgt tcaataggaa ccggaacaat ccaatgttcc agatatgctg
     1681 cttcaggtgt agtggggtaa tacatctcaa ttgcaaaatc agtagttgtt aaattatgtt
     1741 tcttcatcca tctttccata aatttatctg ctttaaaaac agctgaatta actaactcat
     1801 taaagttttc tgcctcaaat ccacatacta aatattcttt agctggcaac tcaaaagtac
     1861 tatatccttc tactgatgta cctgactcta cctctacacc tgccatatac atacaatttc
     1921 cttcttttgc atttcccata taaagagcag caaattcatt tccacctgga atttgatttg
     1981 gtatattatg tctttgactt ccaagtttca tccataattc ctctactatt gcaattccag
     2041 ttgtttcacc gcccaccaac tggcaaatag gtacttctat ttctattcca acaaatgtcc
     2101 ttggattact caatgtctta cgattgactt caatcactat atcatctgca atcagtggaa
     2161 caccttcatc aaccatagca taatttaaaa ttaattctgg cttaataaag tgattgagtt
     2221 ttactggatt tgctctatat ttttctggag ttaatccata tgctttttta aaggaacgtg
     2281 taaatgtctc atgactagca aatccaaaat ttaaagctac atcaagtata cgattcttat
     2341 gttctgctaa gtattcagaa gctcttgcaa gacgacgaag ctttatatac tccattacag
     2401 tcttatttac caatctttta aatagtcttt gatagtaaaa tggtgataaa tttgctttat
     2461 ttgctaattc attaattgat atttcttctg ataaatttgc ttctattaaa tccaatgtaa
     2521 tttgaataga ctcccatgca tgcatactat aactcctcct gatatttttg ttactcttat
     2581 cctttatagt atcaaaacta gaaaaaatgt tcttgataga tagtgccctt tttaattttc
     2641 ttatatgata gtgctaaatc tcattgcttt attataaaca taaaatgtat aaaaataaaa
     2701 tatagcaaat ctttaggtaa gatttgctat attatatatt ttattaaact atttttaaat
     2761 actccattta tttccttaag aaagaactct ttcatatcat ctaattgttc tttatcatac
     2821 acgtacttac catatccaaa ttgaccgtat ttaaactttc tatcctcatt ttccattggc
     2881 aatagtgtat ctggataaat ctctagtatt ctattttttg ctttagttgt atatctatga
     2941 gatataactt caaatataat ttttttatca aaatcctttg gtaaagcttc ttttatactt
     3001 ataatcagat ttttatactc ttctttccaa ccttcatata aaaacacagg tgctattatg
     3061 aaaccaatct gataaccagc cctagcaagt tttacagctg actctattct tttatttgca
     3121 gaggctgtat aatgctcatg ttcatttata attctaggag tattgatact aaatcgtatt
     3181 tctgtatgat tattatgttc agcatccaat aagctatcca catcactgta tttagtaaca
     3241 aatctaaatc ttcctttttc ctctcttcca aaaaactcta ttgttttctt taaagaatta
     3301 gtatatggct ctactggaac tgggtctgat gtagctgccc cttcaaatat ggttacctgt
     3361 ggctttctat cttcaatata tttttttgtc tgttcaagta tttcatctac atttacaaac
     3421 acccttataa atggtttatc acctaattgt gtgttcaaat aacaatactc acatcttccc
     3481 atacatccac tgacaagtgg tagttgataa ttagcagaag gtttacatgt ttgaaacttc
     3541 agactttttc taatcccaac taccatagtc tgcttcccac ttctatattg ttcatataga
     3601 ttttcacctg gaatatgttc tttaatccta tttgatgata tattaattat ctctacatct
     3661 ttattatctc taaatttatt gtatattttt ttacccacat cagtgtctaa agttcctttt
     3721 tcgaatatta ttcttttggg attaaacatt tatttcacct ctacttaaag tttttaaatc
     3781 gaagaatttt ataaaatatt acttaattaa tcttcccttt tttaaactcc tttatttatt
     3841 agaaagtaaa ataaaagctt aagacaaatt ta
//
LOCUS       DAMJVI010000190         3831 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.196, whole genome shotgun sequence.
ACCESSION   DAMJVI010000190 DAMJVI010000000
VERSION     DAMJVI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.196"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            32..700
                     /locus_tag="PC545_003602"
     CDS             32..700
                     /locus_tag="PC545_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458313.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     NGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKEEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSGKEKYEGLWKDDKMHGIGKYTYRDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGVLYKNDGNKQAGIWEDDEQCKSLDFKDLNNNKINNVYQNRLNNN
                     KLKV"
     gene            859..1365
                     /locus_tag="PC545_003603"
     CDS             859..1365
                     /locus_tag="PC545_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HDF2458314.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLDLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            1493..2509
                     /locus_tag="PC545_003604"
     CDS             1493..2509
                     /locus_tag="PC545_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HDF2458315.1"
                     /translation="MGYYNDNDNNKIRRKKISNNKDSNKKSKHSYTSANKKNKSPNTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPNDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGASTIDMQVSKNLLTS
                     NDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVSE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQVIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            2863..3567
                     /locus_tag="PC545_003605"
     CDS             2863..3567
                     /locus_tag="PC545_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HDF2458316.1"
                     /translation="MKKFVIEDAFWNLFPSAKIGVVICNDIDNSIKDEDYKKIILKGE
                     EEALKYLEDSEFSNNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENSFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALNELAKIVQEKLGGN
                     CKISILDIDNKEVSVD"
BASE COUNT         1644 a          326 c          658 g         1203 t
ORIGIN      
        1 tttaaataaa ttttttaaag ggtgattttt tatggaaaat ataactagtt attttacaac
       61 aatactatgt gtatttatat gcttatcatc tgtatttata tttactcaac tagctagagt
      121 atttattaat aagaagaaga ttaatcaaaa aattaaatca agaaacggat ttaggtatga
      181 tagagatttt attgaagcca gaagagaaga aatacatata aaggataaca ataataataa
      241 aaataagagt aacaataaga aattaaagga agaaaaagta tttaaatacg ataatggtga
      301 tttatataaa ggtgagtttg ttgatggaaa gaaaaatgga tttggaatat atattttttc
      361 aggtaaggaa aagtatgaag gattatggaa agatgacaag atgcatggaa taggaaaata
      421 tacttataga gatggttcaa tatatacagg tgaatttaaa tacggattaa aaaatggttt
      481 gggaaaatta acatatccca ataatgacat ttataaggga tattttttag ataataagaa
      541 aaatggtaag ggtgtattat ataaaaatga tggaaataaa caagcaggaa tatgggaaga
      601 tgatgaacaa tgtaaaagtt tagattttaa agatttaaat aataataaaa taaataatgt
      661 ttatcaaaat agattaaata acaataaatt aaaagtttaa ttattctaat aaaaaaatct
      721 ttatttatga tggaatatag gatatatcta tgttgtaatt tgtaaaatct tcctatatca
      781 tataaaatta agtattgatt agtctattac ataaatatag aatataaata tatccattta
      841 ataataagga gaagagatat gaaagagtat gtagttgtgg atttagaaac tacaggatta
      901 gatccatata aaggttgtga aataatagaa ataggaataa cagaaataaa aaatgaacaa
      961 attgtaaaaa actattcaag gttgataaaa cctaagggga taatttcttc atttataaca
     1021 gaattgacac atataagtaa tgaaatggta gaaaatgaag aaccactaga attagtttta
     1081 cctagattta gaaaatacat aggtgataga actataattg cacacaatgc gaaatttgat
     1141 ttaaaatttt taaattacta tcttagaatg ttagatttag aaccaataaa caattatata
     1201 tgtacagtag agttattaaa aaaatgtaaa agttataaag gaaaaaacaa gaaacttgaa
     1261 acagcttgtg cttattacaa catagagaat ataaatgctc atagagctga cagtgataca
     1321 ttagcaactg caaaactatt tttgaaaata aaagatgaat attaattata aatatagaga
     1381 aaattagtga aaatatcctt tataaaattg acccttaagc ttacaatttt gttctttgat
     1441 ttattttgta gaacaatgta taatgtacct tatatgaaga agggagacta aaatgggtta
     1501 ttacaatgat aatgataata ataaaataag acgtaaaaaa attagtaaca acaaagatag
     1561 taataaaaaa tctaaacatt cttatacttc ggcaaataaa aaaaataaaa gtcctaatac
     1621 aagttccagt aaaagaatga gtgaaaaagt agattcacag aatcttataa aaagagatag
     1681 agatagtttt acaagagata atgtaagacc tttaaataca gattacaatc aaagagaaag
     1741 aagaagagtc gagtataaac aaagagagaa aaaattaaaa agtaaaaaaa gaaaaaaaca
     1801 aaaaaggatt agaatagtta gggcattatt atctattatg ttagttgcac taattatatt
     1861 ttcagtggca ggtgcaatat ttacagtttc agctataaaa gggtcaccag aagttactaa
     1921 aaagttaatt aggtctaaat atataagtag tgaggttgta agtataaaac aaatgccaaa
     1981 tgatttaaaa catgcaatag tttccataga agatgagaga ttttataagc ataaaggagt
     2041 agatgttatt tctctagtta ggtcagtatt aaacaatgtg tttacagata caacacaagg
     2101 tgcaagtact atagatatgc aagtttcaaa aaacctgctt actagtaatg ataaaactat
     2161 gaagcggaaa ataagggata tgtataatgc taagcagatg aataagatta tgactaagga
     2221 tgaaattctt gaagcatatt taaataatat gtatctaggt aaaaatgcat atggagctgc
     2281 aaagggagct gaagtctact ttggtaaaaa agttagtgaa ttaaatttag ctgagtgtgc
     2341 tatgttagct ggtatgacaa ataatcctgc aaggtatatt gaccatggag aggctaagaa
     2401 gagacaagtg attgtactgt ataagatgca tcagcttggt tatataactg ataaggagta
     2461 tagaatagca aaagctgatg acactccatt taaatcagaa attgattaaa attgtaagaa
     2521 tatagtattt tcaatagttt aaacaaacaa aaatgtaata aaaaaggtga tttgccaccc
     2581 atttgccacc ataattataa tctaatggca aattgtttta atgtacaaac tagataatag
     2641 aatattgtct taaatgctat attatgctaa aatttaattg tattttgtaa attaaaaaac
     2701 ttataattta gttttctagg gttccgcatt ttataatggt caggtccaag agaaaacgcg
     2761 caattgttgt gtacacggaa ggataaaagc ctgggagata tttaagaata tttctatagg
     2821 cttttttatt tttacatatt ttaaatttga ggaggttttc taatgaaaaa atttgttatt
     2881 gaggatgcat tttggaatct atttcctagt gcaaaaattg gagttgttat ttgtaatgat
     2941 atagataact ctataaaaga tgaagactat aagaaaataa ttttgaaggg agaagaagaa
     3001 gcattaaaat acttggagga ctcagaattt agcaacaacc aagtgattaa agtatggaga
     3061 gaagcattta aaaaatttaa aacgaaaaaa ggagcaaggt cttctattga agcattatta
     3121 aaaagagttc ataatggaaa taatatagga actattaacc ctttagtgga tatctacaat
     3181 tttatctcat taaaatatgc attaccttgt ggaggggagg atatagataa atttatagga
     3241 gatattaggc taactagagc aattggtaat gaggagttta tcccactagg gacagatgaa
     3301 aattcatttc catatgaagg agaaataatt tataaagatg atggtggagc aatttgtaga
     3361 tgttggaatt ggcgtgaagc agtgagaaca atgcttactg aagatactaa caatgccttt
     3421 ttatgcattg aattagtaga tgaaagtaga tttaaagaat ttgaaaatgc tttaaatgaa
     3481 ttagctaaaa tagttcaaga aaaattgggt gggaattgta aaatttcaat tttagatatt
     3541 gataataaag aggtatcagt ggattaatat ggaggtgctg ttgtaatgga ctattctagt
     3601 tataaagata cttatattta gggtaagaag gtctatttta gatataacga ttatcaaagg
     3661 atataatata gaaaataaaa atgtagaata taaaaagcct ttaaaataag aaaaaaatgt
     3721 attttaaagg cttttattat actctttttt atttgcataa ttaacaataa aaatatattg
     3781 ttaattataa tatttttatt taaaaacgat ataatataat gtattatact a
//
LOCUS       DAMJVI010000191         3829 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.197, whole genome shotgun sequence.
ACCESSION   DAMJVI010000191 DAMJVI010000000
VERSION     DAMJVI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.197"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(52..588)
                     /locus_tag="PC545_003606"
     CDS             complement(52..588)
                     /locus_tag="PC545_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDF2458317.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRVMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEVDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDIEEEN"
     gene            complement(606..1919)
                     /locus_tag="PC545_003607"
     CDS             complement(606..1919)
                     /locus_tag="PC545_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HDF2458318.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEDNIEDIEPYWVCLPMATRTAV
                     GNYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRAAESLQRYCSIVNKQHGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGVAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVKLPYGHTWKSFTYFLLDTLPENIRNSYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
     gene            2274..2714
                     /locus_tag="PC545_003608"
     CDS             2274..2714
                     /locus_tag="PC545_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HDF2458319.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKDGYARLCKLETYLKTQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVLIQIGDKQVPAVEKTLVALTPKA
                     KELWVTRYPCGNNGGWIKYRILEDADLLDIIQYIRAKKSPPKQN"
     gene            complement(2899..3087)
                     /locus_tag="PC545_003609"
     CDS             complement(2899..3087)
                     /locus_tag="PC545_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458320.1"
                     /translation="MNKNNNLVIICMFIGMILGMAIGCAIGISKGNVGITMCYGLIFG
                     MIIGICIGTVIKNSNKKE"
BASE COUNT         1335 a          652 c          374 g         1468 t
ORIGIN      
        1 tatattttaa attttctttt ttaataaatc tttttctttt aataaatcta tttaattttc
       61 ttcttctata tcagagccaa agggaatcca tgcattacca aactctttat ctttaaataa
      121 ataagctaat ccagttattt gcttcaatct aagaatttca tcaacttcca tacccaggtg
      181 ctttgatatc cattcatcag attttccaat ttcacaaagt tcagttataa ttttactcat
      241 taaatccaca ttatgagaac ctcttgcacg attgtgtcga atagtacttg ccattctttc
      301 atcaagagat ttatcaatta ctgaaacagg aagaagtcca ttttctctat catatatatc
      361 ctcattttca agcattactc tatatctatg aaaaccatct acaataatat acttatcctt
      421 ttcctcatta taataacaga caattggcat tgtataccca tcttctttta tacttttata
      481 caacaactgc atttctggtg gggctactgc atttggatta tagtcgttag attctatctt
      541 ttcgattggc actgcgatga ttccatatac aggactttta tattccatag catcttaact
      601 cctatctaat gaattttttc atatttcttc ttaatatatt ccgttctaat tctctgttct
      661 tttgtaggac caaatcccat aaatctacac atatggtcat tttttaaaat acaataacac
      721 attcttttcc aacttggtat atctttggta aatttaatat catccgtatc atcaggtata
      781 tcatccaaaa atatgactct actcttttta tcttttgtat gatttgaaac tccatttaat
      841 tctattttat aaccttttct caacaattca cgtataacta tttctgacaa accaccacct
      901 gtattctgcc aaaactcaat agatgttgaa aattttttaa tatagctatt ccttatattt
      961 tcaggtaagg tatcaagcaa aaaatatgtg aaacttttcc atgtgtgtcc atatggcaat
     1021 ttaacactac gatatcccat agcttttgtt ttgccataaa tagaagtaaa attaactcct
     1081 tgtactctag caaccaattt cacccataca tctgggtcca atattctgta taaatttaaa
     1141 ctctcttttg ctgaatcatt aaaaggtgat gccactctca tttttcttgg agctactcca
     1201 gctttataga ataagtcata aattttatta tagtcatagt caaatttata gtgtgctaac
     1261 catatatctt ctatttccca atcatatata ggagatgccg tccagacatt tttaaattcc
     1321 ttagttatcc agcactttcc tttatatcca tgctgtttat tcacaataga acaataacgt
     1381 tgtagagatt ctgctgctcg tgttcctaat aagcaaacaa cttttgcacc ttcatgtata
     1441 tctctaatcc atctaccaaa ttgttttgcc aaactttctt gtctcatttt atatttataa
     1501 taagtaaaag gattattttt ttgatttatt atgtattctt tgactggcat ttcccttacc
     1561 cataactctt ctttctcatc atcccatgga taccaataca tttcataatt tcctacagca
     1621 gttcttgtag ccattggtaa acacacccaa tatggttcaa tatcttcaat attatcttca
     1681 aatacttttt caacatattc agtagtcaaa ctatattgag cctcaaaatc ttgatgaaat
     1741 actcctattt tggaacttat attattcttt ctcttataat ccaaaactaa atttagcagt
     1801 aaaccactat cttttcctcc gctaaaagat acatagatat aatcaaattc attaaaaaga
     1861 taagaaagtc tttcttgaaa agcttcaaat acatcggatt ctaaatatct cttatccatt
     1921 tgaattatcc cctttcaatt agaagtttat aaaaccttac aatgcaatat tattatgctt
     1981 ttttagcagt aatcagcata aattatacat tatttaaata cacaaatcca actaactaca
     2041 tataattata atgtaattat acttttgtat tataaactat attttatcat tattttacat
     2101 ttttttccaa tatatataaa tatcatattt ttctattagc taaaactaat tactttatat
     2161 ttctacgtat tagtataata atacataaaa acttgaataa tgcataaaaa cttgacatac
     2221 tgttgtcata attactttgc tataattaaa agtaaaaatg gaggtaaatt ctaatgtatg
     2281 aacgtatgct tgataaaagc aataaaccct gtttgtctca aataatagaa tatataggta
     2341 aagatggata tgcacgattg tgtaagttgg aaacttactt gaaaactcaa tataatttat
     2401 caagagaact tcgatttcct tttggaaaca attatggttg gggttacaaa tatagccata
     2461 aatcttttca tttattttat gctttttttg aaaaaggtgc atttactgtt ctgattcaga
     2521 taggtgacaa gcaagttcca gcagtagaaa aaacgttggt agctttgaca ccaaaagcta
     2581 aagagctctg ggtcactcgt tatccttgtg gcaataatgg aggctggatt aagtatcgta
     2641 ttttagaaga tgcagactta cttgatatta ttcaatatat aagagcaaaa aaatctccac
     2701 ctaaacaaaa ttaactttta ttttttgtga tttaaatata gtgtatataa attccattct
     2761 ttccatataa aaaaaataat ataaaaaata tctattagta taacttattt agtatgtctt
     2821 attctacttt ttacagataa ttaaaaagtt ttatcataaa taaatcataa ctagacatcc
     2881 tagtaaatca aaattaattt attctttttt attagaattt ttgataacag tccctataca
     2941 tatgccaatt atcattccaa aaattagacc ataacacatt gtgataccta catttccttt
     3001 agaaatacct atagcacaac ctatagccat tccaagtatc atgcctataa acatacatat
     3061 aataactaaa ttgttgtttt tattcatagt tttctccatt caatatattt taaattgcct
     3121 agctcgcctt cttagcttaa aacaaagaaa tataaattaa ctttctttca ttctaatatc
     3181 tgcttgtata aatgtcaaac aacacatttc ttgtcataac ctcctctttt tgaagtaagt
     3241 acaatctccc agacgcaata atatcaattt cctaaaacca tttaagtaac ttttatattt
     3301 acataatatt tacaatcata aactatattt ttattcattt atacatcata aacactccct
     3361 actaatcatt attatactta aaataatgtt catttataat aatatatcta acacacttta
     3421 attgaaaaaa ataaaacttt gcctttcatt cccatatata aataagtata ctttttgatg
     3481 ctctattaca aatcaactaa attaattgat ataaattatc attttctgtt gaaaatacca
     3541 ttcgcacatg ctatacttaa ttgcataaat ggtaataaat gtcatatgat tttacaaatc
     3601 tattaataac aattattact tttactgtat tttaaatttt ttggactaaa tatcaagaac
     3661 tttctaaaat acaaagtaca aaaaatttat ataagttata aatttatatg aactagataa
     3721 gctcaaacaa tatgttatgt ataaactgtg tagtattcgg ttataaattt atataaatta
     3781 tataagctag ataaagtatt acctcaacta tataagctag atatataaa
//
LOCUS       DAMJVI010000192         3785 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.198, whole genome shotgun sequence.
ACCESSION   DAMJVI010000192 DAMJVI010000000
VERSION     DAMJVI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.198"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="PC545_003610"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="PC545_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDF2458321.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="PC545_003611"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="PC545_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDF2458322.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTS
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="PC545_003612"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="PC545_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HDF2458323.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1245..1670
                     /gene="rplK"
                     /locus_tag="PC545_003613"
     CDS             1245..1670
                     /gene="rplK"
                     /locus_tag="PC545_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDF2458324.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1741..2439
                     /gene="rplA"
                     /locus_tag="PC545_003614"
     CDS             1741..2439
                     /gene="rplA"
                     /locus_tag="PC545_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDF2458325.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2491..2619
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2662..3168
                     /locus_tag="PC545_003615"
     CDS             2662..3168
                     /locus_tag="PC545_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDF2458326.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3226..3591
                     /gene="rplL"
                     /locus_tag="PC545_003616"
     CDS             3226..3591
                     /gene="rplL"
                     /locus_tag="PC545_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDF2458327.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
BASE COUNT         1531 a          452 c          749 g         1053 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aagtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacctattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagttat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagacacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag aaaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atattataga aaaatctgag gaggtgcgct caaaatggct aaaaaagtta
     1261 taggtcaaat aaaattacaa atacctgcag gaaaggctac tccagcgcca ccagttggac
     1321 cagcattagg acaacatggt gttaatataa tgggattcac aaaagaattt aatgctaaaa
     1381 ctgcagatca agctggaatg ataataccag ttgttataac tgtatatcaa gatagatcat
     1441 tcagttttat aacaaaaact ccaccagctg cagttttaat taaaaaagca ttaaacttaa
     1501 aatcaggttc tggagaacca aacaagaaaa aagttgctaa aatgacttca gctcaagtaa
     1561 gagaaatagc tgaattaaag atgcctgact taaatgctgc atcagtagaa gctgctatga
     1621 gtatgatagc tggtactgct agaagcatgg gtgttgtaat cgaagactag ttaaaagata
     1681 cacttttttt tataaagtgg gaggctagaa gccgaaatta accacgagga ggaaaataaa
     1741 atggctaaaa aaggtaaaag atatgcaggt gcattacaaa aagtagatag aactaaattt
     1801 tatgatgcat cagaagcatt aacattagtt tcagatatag ctggagctaa atttgatgag
     1861 acagtagaag cacacattaa attaggtgtt gactcaagac atgctgacca acaagtaaga
     1921 ggtgcagttg tattacctca tggaactggt aaaacaaaaa gagttttagt ttttgctaaa
     1981 ggtgaaaaag ctaaagaagc tgaacaagct ggtgctgact ttgtaggagc tgaagaatta
     2041 gttcaaaaaa tacaaggtga aaactggttt gactttgata tagtagttgc tactccagat
     2101 atgatgggtg tagtaggtag attaggtaga gtattaggac ctaaaggttt aatgccaaac
     2161 cctaaatcag gaacagttac atttgatgta gctaaagcta tagatgaaat aaaagctggt
     2221 aaagttgaat acagattaga caaaactaac ataatacacg ttccagtagg aaaagtatca
     2281 tttggtggag aaaaattaac tgaaaacttt actgcattaa tggatgctat aataaaagct
     2341 aaaccagctg ctgctaaagg acaatattta agaagtataa cagtagcttc ttcaatggga
     2401 cctggagtta aaataaaccc agcaagaaca gctgaataat aaaactgttg acacaggttg
     2461 acaaaaatgt tattatatca cttgtcaatg taaaaaagaa tagatttgcc gtagatagtg
     2521 ggtgcgaaag cttaatatcc cgccgaggtt ttagataaat tgagattact aaagcttttg
     2581 atgtctactt ttggcaacaa aagcttttta taatgttgcg gtaaatacta agcctatttg
     2641 gtgaaggagg tgcacatcta tatgagaaaa gctatagaaa ttaaatcaga agttgtttct
     2701 gagatagttg aaaagttaca aaaatcttct gctgctgttg ttgtagatta caaaggatta
     2761 acagttgaag aagtaactga gttaagaaaa caaatgagag aagctggtgt agattacaaa
     2821 gtatacaaaa atactttagt tagaagagca gctaaagaag ttggtataga gcaatttaat
     2881 gatgaattat tagttggaac taatgccata gcatttggat atgatgatcc agttgctcca
     2941 gctagaatat taaaaggatt catggattct catccaaaaa tgaaattaaa gatgggtatt
     3001 gtggaaggtg cattctatga tgaatctaag attgttgaaa tggctaacat accatcaaga
     3061 gaagttctta tcgctaaatt acttggtagc ttaaaagctc cagtatcaaa cttcgcatac
     3121 ttaatagatg caatagcgaa aaaggcagaa ggtcaagaag aagcttaatt gcttaactaa
     3181 aaaattataa atattaattt gaaataaaat tcggaggtgc taaaaatgac aatagaacaa
     3241 atattagaag ctatagaaaa tatgaaagtt ttagaattaa atgaattagt taaagctgct
     3301 gaagaaaaat tcggtgtatc agcttcagct ccagtaatgg tagctggtgc agctgctggt
     3361 ggaccagctg ctgaagaaaa aactgagttt gatgtagtat taactgacgt tggttcttca
     3421 aaagttggag ttataaaagc agttagagaa ataacaggat taggattaaa agaagctaaa
     3481 gaagtagttg acaatgctcc taagacagtt aaagaaggag cttctaaaga agaagctgat
     3541 caaataaaag agaaattaga agctgctgga gctaaagtag aagttaagta gtttctttaa
     3601 aaagataccc attgggtatc tttttttatt tataaaaaac tataaagaaa gtatttggaa
     3661 ttataaagaa agtattttta taaaataaaa aaaatttata taatagtact aaattaaggg
     3721 tatcatatat ggtgtaatgg tattttaatt taacattatt atcaaattaa aatataaaca
     3781 attgt
//
LOCUS       DAMJVI010000193         3782 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.199, whole genome shotgun sequence.
ACCESSION   DAMJVI010000193 DAMJVI010000000
VERSION     DAMJVI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.199"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            83..1195
                     /locus_tag="PC545_003617"
     CDS             83..1195
                     /locus_tag="PC545_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDF2458328.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDEKTGKRKQKNMGTFDKKREASKRL
                     NEVKESLYNDGFLVPSEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPVHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDSIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSVRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVMSKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(1403..1879)
                     /locus_tag="PC545_003618"
     CDS             complement(1403..1879)
                     /locus_tag="PC545_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458329.1"
                     /translation="MVKKRLVAVVISLAMISVGNFSYAHSGGHNSSGDHHNKNKSELT
                     RYYCGGHSAHQHNKGFCPYASTKENSGVTISSVHNEKNHKNYFAEKGYNKGYEDGCKG
                     LYNLSVTEAKSDNLYEKEILKSSYEKGYLIGYNEYKAKKVDDVMKKMLDIPMLASI"
     gene            complement(2078..3703)
                     /locus_tag="PC545_003619"
     CDS             complement(2078..3703)
                     /locus_tag="PC545_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2458330.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLESKEIQDQLAKMYGKDK"
BASE COUNT         1310 a          611 c          424 g         1437 t
ORIGIN      
        1 tgtatgcaac tctatttcaa aattattttg tgatatatat atgttataat aaatgtataa
       61 aattttgatt ggtgggttat atatgaatgc atttattaga aaaagaaata aaaactatgt
      121 tgtttacctg gaatttgaag atgaaaaaac aggtaaaaga aaacaaaaaa atatgggtac
      181 ttttgataaa aaaagggaag caagtaaaag actaaatgag gtaaaagaaa gcttatataa
      241 tgatggtttt ttagtaccaa gcgaaattac agtgtcagaa tttctattag attttctaaa
      301 taaatatagt gaaaatatat ctttagctac atataataat tatgttagta tttgtaagaa
      361 ttacattaat ccatctattg gaaaatataa aatacaagac ttacatccgg ttcacataca
      421 aaactacata gataaactat cttataaact aaatcctcaa tcaattaaga ttcatataaa
      481 tatattgaaa cttgcaataa aaagagctta tagactcaaa ttaataaaag aaaatgttat
      541 ggatagtata gaggctccaa gatataaaaa atttaaaaat gagatttatg atagagaaca
      601 aatgataaaa ttattagatt tagcaaaaaa tacagaaatg gaattaccta ttaatttggc
      661 tattggatta ggtcttagaa tttcagaagt tttaggtcta acttgggata atattgattt
      721 tgaagaaaat acaataacag taaataagat aacttcccga attaatggtt ctgttattct
      781 caaagagcca aagacagaaa gctctgttag aaaaatatct tctcccaaag aattaatgtc
      841 tttattaaaa gaatataaaa taaaacagaa taaaaattta ttaaaatctt ccattagaaa
      901 taataataat ttgttgtttt tcaataagaa atgtgaacct attgctgaag atgttatgag
      961 taaaaaattt aaaagatttt tagaaaagaa tgaattacct catattagat tccatgattt
     1021 aagacactca catgttactt tacttataaa ttctaaagta cctataaagg ttatatccga
     1081 gagagtagga cactcaaata taagtactac tctaagcgtt tattctcatg tacttaaaga
     1141 aatggataag gaggcttctg acaaaatttc tgaaagttta tttaatgcta attaatatta
     1201 atttatctta gctttaaatt agcttagctt attgagatag attcattaat attaaatgta
     1261 tctatctcaa attaataact acttaaataa tctgttttat tttaatttat cgcctcagtt
     1321 attatacttt tttacgaaaa attatacttt acaataaaaa agatattccc ttatagaaaa
     1381 tatcttttta aatactatat tattatatac ttgctaacat tggaatatct aacatcttct
     1441 tcattacatc atctactttt tttgctttat attcattgta tccaattaaa tatccttttt
     1501 catatgaact ttttaaaatc tctttttcat ataaattatc agatttggct tctgtgacac
     1561 ttaaattata aagcccttta cacccatctt cataaccttt attataccct ttttctgcaa
     1621 aatagttttt atgatttttt tcgttatgta ctgaagatat tgttactcca gaattttctt
     1681 ttgtactagc ataaggacaa aaacctttat tgtgttgatg tgctgaatgt cctccacaat
     1741 aataacgtgt taactctgat ttatttttat tatgatgatc tcctgatgaa ttatgccctc
     1801 ctgagtgagc atatgaaaaa ttccctacac taatcatagc taaactaatt actacagcta
     1861 ctaacctttt cttaaccata atctattccc cccaaaatgt tatatatttt tattatatca
     1921 tctactatat tatatttaaa ttcttaatat acaaaatatt actctattct gctaataaac
     1981 atgtttaaac aacataaaaa acctcctaat atttgattaa aaatttacac actcatgcat
     2041 agcatgtctc aaatattaaa aggttttata ctaataatta tttatctttt ccatacattt
     2101 tagctaattg gtcttgtatc tctttacttt ctaaatactc atcaaagtcc atttttctat
     2161 ccattattcc atttggagtt atctctataa ttctatttgc tatagtagat ataaattcat
     2221 ggtcatgaga agtaaataaa agcactcctg ggaacttttc aagtccttta tttacagaag
     2281 ttatactttc taggtctaag tggttggtag ggtcatctaa tacaagtaca tttgcatttg
     2341 aaagcattag cttactaagc atacatctaa ccttttctcc tccagatata acttgagctt
     2401 cttttaatgc ttcttcacct gagaataaca ttctacctaa aaatcctctt ataaaacttt
     2461 cacttttttc ttcagagaac tgtctaagcc agtcaaccaa agaatattct actccgtcaa
     2521 aaaacttatt atgatttttt ggaagataat cttgagaagt agttactccc catttatact
     2581 ctccactgtc tggttcaagt tctcccataa ctatgttgaa aagtgcagta gtagctattt
     2641 catctcccat aaatactact ttatcatctc tatccaatct aaatgacaca ttatcaagaa
     2701 ccttaacccc atctatagtt ttagttaaat tgtgtacttc taaaacttca ttcccaattt
     2761 ctcttgcagg tgtaaatcct acatatggat atcttcttct tgatggttgt atatcttcaa
     2821 cttctaattt ctctaactgt ttctttcttg atgttgcttg tttagccttt gaagcattag
     2881 aactaaatct agctataaat tcctttaatt gagctatctt ttcttcagtc tttttatttt
     2941 ggtccttagc cagttgtaaa gccaattgac ttgactcata ccagaaatca tagtttccaa
     3001 cgtacatttg tattttacca aaatctacgt caactatatt agtacatatt tgatttagga
     3061 aatgtctatc atgtgatact attataacta tagattcttc taaatccatt ataaaattat
     3121 ttaaccagtt tattgattta aaatctaagt ggtttgtagg ttcgtccatt aatagtattt
     3181 caggcttacc aaaaagtgat tgtgctaaca ataccttaac cttttcccca ccaactagtt
     3241 ccttcatttg tttgtaatgc atatctttcg ttatgccaag acccataagt attttctcag
     3301 cattagtttc tgcatcccat ccgtcaagct ctgcaaactc accttcaagt tcggcagctt
     3361 ttatcccatc ttcttcatta aagtcttcct tcatatacaa tgcatctttt tctttcatta
     3421 tgttccaaag tctctcatga cctcttataa caacatttaa aacagtttct tcttcatatt
     3481 caaagtggtc ctgttttaaa actgacattc tttctttatc agttatagat atactacctg
     3541 tattaggctc tatttctcct gataatattt ttaaaaaagt agattttccc gcaccattag
     3601 ctcctattat tccatagcaa ttacctttag tgaattttaa gttaacatct ttaaatagtt
     3661 ccttatcacc aaatctaagt ccaacacctg taacttgtaa cattaatata ttttcctttc
     3721 tttttgctca taatttattt tatcataaaa ctttgataac atttttatta tatattatca
     3781 cg
//
LOCUS       DAMJVI010000194         3732 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.200, whole genome shotgun sequence.
ACCESSION   DAMJVI010000194 DAMJVI010000000
VERSION     DAMJVI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.200"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            231..1811
                     /locus_tag="PC545_003620"
     CDS             231..1811
                     /locus_tag="PC545_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDF2458331.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTYGKEVNVFLVGNLEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGEENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYDTSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRRDKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     NKLLNSSDAIGFGPGMGDNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNLVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDKLSKAMYTVN
                     ATDLISNISLTMKELLDI"
     gene            1989..2435
                     /locus_tag="PC545_003621"
     CDS             1989..2435
                     /locus_tag="PC545_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDF2458332.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVILIFLSSFIESI
                     FLQILIFAVISIAMLVVATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVDATVNVTNQ"
     gene            2456..3499
                     /locus_tag="PC545_003622"
     CDS             2456..3499
                     /locus_tag="PC545_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDF2458333.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHFLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQMATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEVLD
                     IKEVLNDNESLKK"
BASE COUNT         1511 a          343 c          700 g         1178 t
ORIGIN      
        1 gatatttaga taattagtgt aaataggttt taaatcaaca taataactta aattatatgg
       61 tataataaat taaataattg ttttaattca aaattttttg ttattttaaa ttatttcaaa
      121 actcttagtt ttagtgattt tatttatgat atttatggga ttaaaaagat ttcaatagat
      181 atttttggta agtatttaag aagtctttaa ctttatagga gtgataattt atgctaatag
      241 gtacatctaa attaagcaga aatgttgata ctgcatgtgt agaagaagta gggattccaa
      301 tgattgtatt gatggaaaat gcggttatat ctgcaatgaa aaatatggat atagacatgt
      361 ataatagtta tacagttgta tgtggtgttg gaaataatgg aggagatggt cttggaatag
      421 caagacatct aaatacatat ggaaaagaag taaacgtatt tttagtaggt aatcttgaaa
      481 aactaagtga atgctctaaa ataaattaca atgtactttt aaatatggga attaatatta
      541 taaatattaa taaaaatgat ttaagtgaaa aaaattatgg agaggaaaat gaagtagata
      601 aaattattaa agttgagaaa caaaatatta taaaaaataa aaatttaaca atttttaaag
      661 atagagtatc taaaagtgat gttgtagtag atgctatatt tgggactgga ttaaaacgtg
      721 atataactgg tatttttaaa gaagttattg acatagtaaa cgataatagc aaaaatacgt
      781 attctataga tattccttca ggtatagata gcgataatgg aaatgtatta ggaacttgta
      841 ttagagcaaa caaaacgatt agctttgagt tttataaaag aggttttcta aactatgaca
      901 ctagcgcact tataggtgat gtagttgtag aaaagatagg tgttccagat tttattacaa
      961 gaaaatacca tgatgaagag ttcataactg ataagacttt tgtaaaaaat aatattagaa
     1021 gaagagataa gcatggtttt aaaagtgatt ttggaaaagt atctatagtt gcaggttcaa
     1081 aaggatttta tggtgcaagt ttcatagcaa ctgaatctgc tgttaagagt gggagtggac
     1141 ttgtaacatt aattagtagt gaagatgtgc aagaaaaggt atctattaga cttacagaag
     1201 caatgacttt aaattttgaa gaagaaagat taaataaatt acttaattct agtgatgcaa
     1261 ttggttttgg tccaggtatg ggagataata gtcaaacatt tgataaatta cttaagatag
     1321 tagaaaattc taattgccca atagtattag atgcagatgg attgaacgtt atgaaggaca
     1381 gatgttataa gtttttagaa tggaaaaata gatttgtaat tacacctcat ttgggagaaa
     1441 tggccaggtt gacaggagat tcaattgggt atataagaga acatagagta gatgttgcaa
     1501 aagaatttgc tcaaaaatat aatctggtgg tgcttttaaa aggttatcaa actgtcataa
     1561 cagatggtaa aaaaacatat ataaatccta caggaaatag ctgtatggca actggtggaa
     1621 tgggtgattg tttgcttgga atgattactt catttatagg tcaaggtatg gatatattgg
     1681 aagctacagt tagtggagca tatatacatg gctatatagg agataaactg agcaaagcaa
     1741 tgtatacggt aaatgctacg gatttaataa gtaacatttc attaacaatg aaagagttgt
     1801 tggatatata attggtgata tttgatacta ctttgtaaca aatttaacaa agtatgttga
     1861 tataatacta ttaattaatt aattaattta ttttattgtt ttgtaattta aatttatatt
     1921 caaaaacatt gtataaacat ataatttata tagggtttta taattcatat gaaaagaggg
     1981 tagatagtat gaagttaata tggttgatag tagcagtctt atttggaata gcagaaatgt
     2041 taacaccaag cttaacattg atatggttta gtgtaggagc tgtaatactt atatttttaa
     2101 gtagctttat agagagtatt ttcttacaaa ttttaatatt tgcagtaatt tctatagcaa
     2161 tgttagtcgt agctactaaa aaaatagtaa aaaaagataa aggctataaa tctaacacta
     2221 atttacaagc aatgatgtct aaaaaaggta ttgttacaga agagatttct ccaaataaca
     2281 caggtctagt tgtagttgaa catgaaacat ggacggctat ttctatagat ggtgaaaaga
     2341 tagaaaaagg ttctacagta gaagtattaa aaattgaagg ggtaaaatta gttgttaaaa
     2401 aagtagacgc cacagttaat gtgacaaacc aatagaataa aggaggattt atagtatggg
     2461 tactaaaatt gttttaagta ttgtattaat agtagtagtc gtagcaataa gtttaacttg
     2521 tataagagtt ataaaacaat ctaaagtagg tataataatg agacttggta agtttcaaaa
     2581 agtagctgaa acaggtgtac atttcttaat accattttta gataaaatgg catatgtaat
     2641 tgaccttaga gaaattgtaa tagattttcc acctcaacca gttataacta aagataatgt
     2701 aactatgcag attgatacag ttgtatatta taaagttaca gacccagtta gatatgtgtt
     2761 tgagatagct aatccaatag ctgctattga aaatttaaca gctacaacac taagaaatat
     2821 aattggtgaa cttgatttag atgaaacatt aacatcaaga gatataataa atgtaaaaat
     2881 gagaactatc cttgatgaag caacagataa atggggtata aaagtaaata gagtagagtt
     2941 aaaaaatata atgcctcctc aggatattca agttgcaatg gaaaaacaaa tgagagcaga
     3001 aagagaaaga agagaggcaa tacttcaagc agaaggtaat aagtcagctg caatacttca
     3061 agcagaaggt gaaaaacaat ctgcaatatt aacagcagaa gccaaaaaag aagctatggt
     3121 acgtgtagca gaaggtgaaa aagaatctgc tatattagtg gcagaaggtg aagctgaagc
     3181 tataagacag acagctattg ctaaggcaca aggtgaagct gaaatgataa aaagaactca
     3241 aatggcaaca gcagaaggtt taaaattagt tttttcagca atgaaagagg ctgatataga
     3301 caataatatt ctagcattaa aatctatgga agctcttgaa aaaatggctg aaggtaagtc
     3361 aacaaaactg gttttacctt cagaagcagt taatttccta ggaacattca aaggaataaa
     3421 ggaagttatg agtgatgata acaaagaagt acttgatata aaagaagttt taaatgataa
     3481 tgaatcatta aaaaaataaa agagttttgt tatcatagga aggatatgga aattaaaggt
     3541 cgaattaatt aaatatagca caaaaatgca cgttgaaaag aagaggactt ataaattttt
     3601 aagtcttctt ctttgttttt attagaaaga tgaaattata aaaataaatt aacagaatct
     3661 ttatacaatt tatcctttac taatgtaaaa agtgttatat aatataatat aaaacatata
     3721 gcaaataagc at
//
LOCUS       DAMJVI010000195         3686 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.201, whole genome shotgun sequence.
ACCESSION   DAMJVI010000195 DAMJVI010000000
VERSION     DAMJVI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.201"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            261..620
                     /locus_tag="PC545_003623"
     CDS             261..620
                     /locus_tag="PC545_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458334.1"
                     /translation="MKLRESYTCPLELVHDIIKGKWKTIIIFQLRNGKCSFSELHHSI
                     AGITQKMLLEQLRELKHFGLIDKKTYDGYLLKVEYFLTGQGEKMLSAIKIMQEIGIDY
                     MVEHGMTEILDEKGICY"
     gene            710..1261
                     /locus_tag="PC545_003624"
     CDS             710..1261
                     /locus_tag="PC545_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDF2458335.1"
                     /translation="MKKALLIIDVQNDYFPNGKCQLYKPEIALNTIKDLLKIFRKQNL
                     PIIYIRHESIHGTFFNPNTNGVQIHNDIKPLDTETVIVKHYPNSFYETNLQNKLVEIE
                     VTKLVVCGMMTHMCIDTTIRAAKDYGYEITLISNGCATKELKWNGVNIPADTVQSIFM
                     ASLNQKFANVITSTEFLSNEITR"
     gene            1245..1610
                     /locus_tag="PC545_003625"
     CDS             1245..1610
                     /locus_tag="PC545_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458336.1"
                     /translation="MKLRDEYTCPLELIHDMIKGKWKPIILWRLRLGATSLSKLENDI
                     DGITQKMLLEHLKELIDYGFVDKKTFSGYPLHVEYFLTEDMGNQILEALRIMQHIGIE
                     YLRKNGMENELTEKHVIPD"
     gene            1698..2198
                     /locus_tag="PC545_003626"
     CDS             1698..2198
                     /locus_tag="PC545_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2458337.1"
                     /translation="MYISSIGISNGIIEKKYGSHGVQFNENDVPTYSIPFKIENAPDK
                     TVSFAFILEDKDAYPVTGFIWVHWLGANLTRSELKENESQTAKDFIQGANSWTSILGN
                     QQTREQTSCYGGMAPPDKAHIYELHVFALDKLLDLKNGFYLNELYQEMEGHILEEAVL
                     KGLYEK"
     gene            2188..2703
                     /locus_tag="PC545_003627"
     CDS             2188..2703
                     /locus_tag="PC545_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDF2458338.1"
                     /translation="MKSKPVKVGLRTIKTVLSAFLCFLIDSLRASSVPFYAVIASILC
                     IQPDMENSLSVAKNRELSTIIGGILGMLFLLMERIFLPIQPEILRELTLSILLIPIIN
                     LSVLLKQKKGTFLTCVVFLCVTVTHEKDISPIWFAWNRIVDTSLGIGISLAVNSFPFH
                     KFKVGGTMGKY"
     gene            2697..2978
                     /locus_tag="PC545_003628"
     CDS             2697..2978
                     /locus_tag="PC545_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDF2458339.1"
                     /translation="MLIVEFTEKEDAVFQQAVEWLSSKLAYTPASFNQQKKGFDIDPL
                     TRTFTNEEGTSIYLTAKEFDLLYFLSSHKGQVFTKEQIYEKVWGYDNFY"
     gene            3132..3322
                     /locus_tag="PC545_003629"
                     /pseudo
     CDS             3132..3322
                     /locus_tag="PC545_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            3346..3570
                     /locus_tag="PC545_003630"
     CDS             3346..3570
                     /locus_tag="PC545_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458340.1"
                     /translation="MIGIKKRDIKKALKAGAKAGGSSYADVLADIEATIDEVMNSTDP
                     EVQANFKKYFGNKRPTPEEYIYKITKKTRV"
     gene            3592..>3686
                     /locus_tag="PC545_003631"
     CDS             3592..>3686
                     /locus_tag="PC545_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2458341.1"
                     /translation="MKNEMWVLCPICNNKTRTKVREDTELINFPL"
BASE COUNT         1329 a          500 c          721 g         1136 t
ORIGIN      
        1 ttccccatag aataccttat cgcctaaatc attacgagaa agcccagctt tttcacgggc
       61 ttctctgatt gccagcccta gcggtctgaa atcaaaagta tgtgtttctt ttgtttttct
      121 cattttaaat caccatacat gagtttatac ttcatggttc gtatttagaa tgtgttaaaa
      181 gtgcatatat tagggtaata aaatatgttt tgaaatattg agcttaaaca tgtaagatat
      241 ttcacatagg agatgataaa atgaaactta gagaaagcta tacatgtcca ttagaacttg
      301 ttcatgatat tataaaggga aaatggaaaa cgataattat ttttcaactt agaaatggaa
      361 aatgctcttt ttccgaactc catcacagta tagcaggaat tactcaaaaa atgctattgg
      421 aacaactgcg tgaattaaaa cactttggct taattgataa aaaaacatat gatggatatc
      481 ttctaaaagt agaatacttt ttaactggac agggagaaaa gatgctatca gcaattaaga
      541 taatgcaaga aattggtata gattacatgg tagagcatgg tatgacagaa atccttgatg
      601 aaaaaggaat ttgttattaa actctatcat atacacgaaa aagtaagtaa tttacttaga
      661 aactattatc ttatattatt taattagaat taagagagga tatgagagta tgaaaaaagc
      721 attacttatt attgatgtgc agaatgatta ttttcctaat ggaaagtgcc agttgtataa
      781 accagagatt gcgttgaata caataaaaga tttattgaaa atttttagaa agcaaaactt
      841 gccaattatt tacattcgcc atgagtctat ccatggaaca ttttttaatc caaatacaaa
      901 tggtgtacaa atccataatg atattaaacc attagataca gaaacagtta ttgttaaaca
      961 ttatccaaat agtttttatg aaacgaattt gcaaaacaaa ctggtagaga ttgaggtaac
     1021 gaaattggtt gtttgtggaa tgatgacaca tatgtgtatt gacacaacta taagagctgc
     1081 caaagattat ggctatgaga ttacactaat atcaaatggt tgtgcgacca aggaattaaa
     1141 atggaatgga gtcaatatcc cagcagatac agttcaaagc atatttatgg cttctttaaa
     1201 tcagaaattt gctaatgtta ttacaagcac ggagtttttg tcaaatgaaa ttacgagatg
     1261 aatatacatg tcctttggaa ttaattcatg atatgataaa gggaaaatgg aagccaatta
     1321 ttttatggcg cttacgatta ggtgctactt cattatctaa attagagaat gatattgatg
     1381 gaattacaca aaaaatgctt ttggaacatt taaaggaact tatagattat ggatttgttg
     1441 ataaaaaaac attttctggt tatccacttc atgtcgagta ttttttgacg gaagatatgg
     1501 gaaatcaaat actagaagca ttacgaatta tgcaacatat agggattgaa tatttaagga
     1561 aaaatggaat ggaaaatgaa ttaactgaga aacacgtgat tccagactaa tacctacaaa
     1621 aaagtgggta atttacgttt tgcttcctct ttgttaatat atatctgtaa ttaaacgata
     1681 tacaaggagg aaaatttatg tatatttcaa gtataggaat ttctaatgga attattgaaa
     1741 aaaaatatgg tagtcatgga gtgcaattca atgaaaatga tgtgccaacg tattcaattc
     1801 cgttcaaaat tgaaaatgca ccagataaaa cggtttcctt tgcctttata ctggaagata
     1861 aagacgcata tccagtgacg ggttttatat gggtgcattg gttaggagct aatctaacaa
     1921 gaagtgaatt aaaggaaaac gaaagccaga cagcaaagga ctttatacaa ggtgcgaata
     1981 gctggacaag catattgggg aatcagcaaa ccagagaaca gacaagctgt tatggtggaa
     2041 tggcaccacc agataaagca catatttatg agcttcatgt atttgccctt gataaactgc
     2101 ttgatttaaa aaacggattt tatcttaatg aactctatca agaaatggag gggcacatcc
     2161 ttgaagaagc tgtactaaaa gggttgtatg aaaagtaagc ctgttaaagt tggattgcgg
     2221 acaattaaga cggtactttc tgcgttcctt tgttttttga tagatagctt aagagcttca
     2281 agtgttcctt tttatgcagt gattgcgtct atactgtgca tacagccaga tatggaaaac
     2341 agtttgagcg ttgctaaaaa cagggaactt tcaacaatta tcggcggtat actgggaatg
     2401 ctctttttgc ttatggaaag aatttttttg cccattcagc cagaaatact tcgggaattg
     2461 actttatcta ttttattgat tccaatcatc aatctgtctg tactgttgaa acagaaaaaa
     2521 ggaacatttt taacgtgcgt tgtattctta tgcgtaacag taacacatga gaaagatata
     2581 agtcctattt ggttcgcatg gaataggatt gttgacactt cattagggat tggtatttca
     2641 ctggcagtaa actctttccc ctttcataaa tttaaggtag gtggtactat gggaaaatac
     2701 tgattgttga atttactgaa aaagaggacg ctgttttcca acaagcggtg gaatggctgt
     2761 cctctaaact tgcttacacc cctgcttctt ttaatcagca gaaaaaagga tttgatatag
     2821 acccattaac ccgtaccttt accaacgaag agggaacgtc tatatatctg actgctaaag
     2881 aatttgacct actatatttc ctttccagcc acaaaggaca ggtgttcaca aaagaacaga
     2941 tttatgaaaa ggtatgggga tatgacaatt tttattagtc tggcaataat gaagaaaaaa
     3001 gaattttaga aaagtgaggt gtgcttatgg atttatctat tttggtagta gtattgatat
     3061 tgggtgtggg tattccttta agaaataagg caatcaaaaa atacagataa aatatgaaaa
     3121 agaaaggaaa aatgaaaaaa tatgaatatt tagaaattga ttatactgcg aaaggtgttg
     3181 tattcctttg caccgataaa cataaggaag ttataaatag ctatgcagaa aatgggtatc
     3241 gctatgcggg ttcgtgccta cagaaattga cgcaaaaggc tgtatgcgaa agatagattt
     3301 aatttttgaa aaagaagatt aacgtatttt agggagttgg ttcttgtgat aggaattaaa
     3361 aaacgagata ttaaaaaagc attgaaagct ggtgcaaagg ctggtggttc ttcgtatgct
     3421 gatgttttgg ctgatattga agcaacgatt gatgaagtta tgaacagcac cgacccagaa
     3481 gtgcaggcaa atttcaaaaa gtattttgga aataaacgcc ctacaccaga ggaatatatc
     3541 tataaaatca cgaagaaaac acgagtttaa agaattagta agggtggttc tgtgaaaaat
     3601 gaaatgtggg tattatgtcc tatttgcaat aataagacac gaactaaagt gcgagaagat
     3661 acggaattga taaattttcc tttgtt
//
LOCUS       DAMJVI010000196         3373 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.202, whole genome shotgun sequence.
ACCESSION   DAMJVI010000196 DAMJVI010000000
VERSION     DAMJVI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.202"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..161)
                     /locus_tag="PC545_003632"
     CDS             complement(<1..161)
                     /locus_tag="PC545_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2458342.1"
                     /translation="MKVFLVIDGEPVGKERPRFNLATKRTYTPQKTKDYEELIKWLYQ
                     SKVRHYFTG"
     gene            complement(287..628)
                     /locus_tag="PC545_003633"
     CDS             complement(287..628)
                     /locus_tag="PC545_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HDF2458343.1"
                     /translation="MANIYCENYNCKNYFEDMCMLERIEINNFKECESYLEGKNELYE
                     LENGYTIHPKDLKMVKSKDYSVEVTHIPTGITVKCRSTNSILKNKNKCLEALEEELTK
                     INSHLELEDLR"
     gene            complement(743..883)
                     /locus_tag="PC545_003634"
     CDS             complement(743..883)
                     /locus_tag="PC545_003634"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458344.1"
                     /translation="MNKRIKMKKRLIHKKCDERCVNYDFVISNNLITCNVCRMQIQRK
                     YG"
     gene            complement(886..1257)
                     /locus_tag="PC545_003635"
     CDS             complement(886..1257)
                     /locus_tag="PC545_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458345.1"
                     /translation="MFNIYKVKIKTKRTLEQVRNQSVDFEYSEKGLKNTLKYYNLIDD
                     LKVIVVKFGDEYCLANYNEEDRKIIMEAHYLLEQDEYTGCYINEYERFKKDWENGNCD
                     GEACMVFSDDEIEIIEKLREG"
     gene            complement(1294..1623)
                     /locus_tag="PC545_003636"
     CDS             complement(1294..1623)
                     /locus_tag="PC545_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458346.1"
                     /translation="MKIFLLTILLIIICILANYVKNRIYKKSINNLKYKYSVGEKIIY
                     HQINCYYNRMVGCEILEKCYSTKFRKRNTPLYKVKAYVGDNDTTWVIPEWRIECLATT
                     YGEFPKY"
     gene            complement(1705..2709)
                     /locus_tag="PC545_003637"
     CDS             complement(1705..2709)
                     /locus_tag="PC545_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDF2458347.1"
                     /translation="MIIHKFIIHVLDKNSDTPILNDFEGRVSQDMDLFFQKKIKTVSR
                     AKDIRTAVFNDYSNNLIKKCCEQIIYDESSFLNNSKEIAAYLFDVMKLNATLESCDLA
                     ICLYSQKDEKKVAILKLDYNKSYTHSIEFKDDKFNIQMSKNEINIQETKTVKIAALVG
                     LSGMNDEYHLRVLDKDAEKEEANSKFITEFLNATKVKDDKYKTKMFKSTAENWITNAL
                     GNDIKQAEDVRSILNYTLKEKHEIDINNFVDKTIKDDKLKDSFKEHMEEKGLVEGFSI
                     DKKWVDKKLKKRNIKTDNGFEIKGNLTDFEDPMKYTVRQNQNGSIDIVIKNVTFYEEK
                     "
     gene            complement(2706..2927)
                     /locus_tag="PC545_003638"
     CDS             complement(2706..2927)
                     /locus_tag="PC545_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458348.1"
                     /translation="MSKYVLRWQKGLLLDERKINYSCGSKEMLKQKAELLAKDDKILL
                     VTIDKVEEVIKDTRSQKMTEYYCDGGIEI"
     gene            complement(3006..>3373)
                     /locus_tag="PC545_003639"
     CDS             complement(3006..>3373)
                     /locus_tag="PC545_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2458349.1"
                     /translation="TINARCLKAKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRR
                     IKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYK
                     QAGNAVTANVVYEIAKRMG"
BASE COUNT          984 a          657 c          303 g         1429 t
ORIGIN      
        1 tatccagtaa aataatgtct aactttagat tgatataacc attttattaa ttcttcatag
       61 tccttagttt tctgtggtgt ataggtcctt ttagtagcca agttaaatct aggtctttct
      121 ttaccaactg gttctccatc tattacaaga aaaactttca ttttttcacc ttcttagcct
      181 tcttcctaca ttctttacaa caataaacat ccttagattt ttcttcaaga taaaataact
      241 taccacacca actgcatctt cttcgtttca taagctcact tcctatttag cgtaaatctt
      301 ctagctctaa gtgagagttt atttttgtta gttcttcttc tagagcttcc aaacacttat
      361 ttttattttt taaaatacta tttgtagaac ggcattttac tgtaatacca gttggaatat
      421 gagtaacttc aacagaataa tctttacttt tcaccatttt caaatcttta ggatgtatag
      481 tatatccgtt ttctaattca tatagctcat ttttaccttc aagatagctt tcgcattctt
      541 tgaagttatt aatttcaatt ctttcaagca tacacatatc ttcaaagtag tttttacaat
      601 tataattttc acaatatata ttagccattt aacgcactcc ttttataagt caagcctata
      661 acattctagt ttcattcaca aacttacctt gactatttta ttctatttac ttcttaattt
      721 cttatagttc tcttcacata ctttatccat attttctttg tatttgcatc ctacacacat
      781 tacaagttat aaggttattg ctaattacaa agtcatagtt gacacatctt tcatcacact
      841 ttttatgaat taatcttttt ttcattttaa ttcttttatt catatttaac cctcccttag
      901 cttctcaatt atctcaattt catcatctga aaataccata caggcttccc catcacaatt
      961 accattttcc caatcttttt taaatcgttc atattcattt atataacatc cagtatattc
     1021 atcctgctct aaaagataat gtgcttccat tattattttt ctatcttctt cattgtaatt
     1081 agctagacaa tattcatctc caaatttaac tactattact tttaaatcat caatcaagtt
     1141 atagtatttc agagtatttt ttaatccttt ttctgaatac tcaaagtcta cgctttgatt
     1201 tcttacctgc tccaatgttc ttttagtctt tattttcact ttatagatgt taaacataat
     1261 ttacaatctc cttctccagc caatttttat tatttaatat ttaggaaatt ctccataagt
     1321 cgtagcaaga cattcaattc tccactctgg tataacccat gttgtatcgt tatcacctac
     1381 atatgctttt actttataaa gcggggtatt tctttttcta aatttcgtac tataacattt
     1441 ttctaaaatt tcacaaccaa ccattctgtt atagtaacag tttatttgat gatatataat
     1501 cttttcccct acagaatatt tatattttag attatttata gattttttat atatgcgatt
     1561 tttcacataa tttgctaata tacaaataat tattagcagt atagtcaata aaaaaatctt
     1621 cataagttat tcctcctaac tagtttaaat ttacatcctc ttattattta cttctccttc
     1681 tctagccaat agtcacatga gtacctactt ttcctcataa aatgttacat tcttaataac
     1741 tatatctata gacccatttt gattttgtct tactgtatat ttcattgggt cctcaaaatc
     1801 agttaagtta ccttttattt caaagccatt gtcagttttt atatttctct ttttaagctt
     1861 tttatcaacc cattttttat ctatactaaa tccttcaaca agaccttttt cttccatatg
     1921 ttctttaaaa ctatctttta acttatcatc tttaattgtt ttatcaacaa aattatttat
     1981 atcaatttca tgcttttctt tcaaagtata atttaatata cttcttacat cttctgcctg
     2041 ttttatatca tttccaagag cattagttat ccaattttcg gctgtacttt taaacatctt
     2101 agtcttgtac ttgtcatctt tcactttagt agcatttaga aactctgtaa taaacttaga
     2161 attagcttct tccttttctg catccttgtc taaaacccta agatgatatt cgtcattcat
     2221 tccactcaaa ccaaccaaag cagcaatttt tactgtctta gtctcttgta tattaatttc
     2281 atttttagac atttgtatat taaatttatc atctttaaac tcgattgaat gagtatatga
     2341 cttgttgtaa tcaagcttta atatagcaac tttcttttca tctttttgag agtataaaca
     2401 aattgctaag tcgcaagatt ctaatgtagc attcaatttc ataacatcaa ataaataagc
     2461 tgcaatctct ttagagttat ttaaaaatga actttcatca taaataattt gttcgcaaca
     2521 cttcttaatt aaattgttac tatagtcatt aaatactgct gttctaatgt ctttagctct
     2581 tgatactgtc ttaatcttct tctgaaaaaa tagatccata tcttgactaa ccctaccctc
     2641 aaagtcattt agtattggtg tatcgctatt cttatctaaa acatgtatta taaatttgtg
     2701 tattatcata tttcaattcc tccatcacaa taatattcag tcattttttg acttctagta
     2761 tcctttataa cttcttcaac tttatctatt gttacaagca gtatcttgtc atctttagct
     2821 aataattctg ctttttgttt taacatttcc ttacttccac aagaataatt tatcttacgt
     2881 tcatccaaca ataacccttt ttgccatcta agcacatact ttgacattta ttcacccctt
     2941 attttcattt ttgagagtta caaaacactt caacaatact catactaaaa gacattttgc
     3001 aactcttagc ccattctttt tgctatttca tatacaacat ttgcagtaac agcatttccc
     3061 gcttgtttgt acagttgact atctgagcat acacttgctg ctctttcgta atatttatcc
     3121 ggaaatcctt gcaacctaaa gcattccttt ggtgttaacc ttcttatatc tccatttttc
     3181 aaaattccat gtttatcttg agctgttagt gtgaacattg gttctccgct ttctttaatt
     3241 ctacgaccat tttgtctttt atttacccta tcgggcgtta aaactgcatt aactaaaact
     3301 ccactattat cacaatttct atttgtcaca cctgcattat attttgcttt aaggcatcta
     3361 gcatttattg tta
//
LOCUS       DAMJVI010000197         3320 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.203, whole genome shotgun sequence.
ACCESSION   DAMJVI010000197 DAMJVI010000000
VERSION     DAMJVI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.203"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(28..393)
                     /locus_tag="PC545_003640"
                     /pseudo
     CDS             complement(28..393)
                     /locus_tag="PC545_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(414..1493)
                     /locus_tag="PC545_003641"
     CDS             complement(414..1493)
                     /locus_tag="PC545_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HDF2458350.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     NKSDSKGAVENILKELRGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            complement(1547..2836)
                     /locus_tag="PC545_003642"
     CDS             complement(1547..2836)
                     /locus_tag="PC545_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDF2458351.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGISVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIIATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSTKNKQEIDKAV"
     misc_binding    complement(2999..3210)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1095 a          664 c          366 g         1195 t
ORIGIN      
        1 gtcacgagtg tgcgtagcta ttttttaatc aaactcatat ccaccagata ccaatattga
       61 atctaattct attttttctc ttaagtcttc tggccaatct ataaatggaa gaatggcagt
      121 agtggctggc tttcctattc ttatcatagc cacattatta agtctttttc tttctacaat
      181 agcatttgta caatgagttg taccaagcat agcatatttt attaatgaat ggtcaatctt
      241 actttcatct ataacttttt ttaaggcatt gtaaacacca gtttcaacat cttttgtagt
      301 tgccacctta actgaatgaa ttacttttaa ttccccatcc agtatacaag catctgtatt
      361 tgttcctcca acgtcaattc ctacacgata catcaaaatt tcctcctaaa cttttatttt
      421 ccttgtaatt cttctactgt tttatattcc acatcatatc caaagtatcc aggtccaact
      481 gtctcaattc ctttttctgt tctccatttt tcattacaag gaaaagctac taccataaca
      541 cgagagccat attttattct ctctgtagtt attggaaatc cagtttctaa atctaatact
      601 gctattaaat ctggtgtaat acaaagtggt gtatcacctt ttttggcaat taaattctca
      661 ttttggaata atatagaata ctctccatca tactcattaa tcccagcaaa aaaggcatgt
      721 cctctagtaa atccaccttt caactctctc ttaatatcaa ttacttttcc ttcaaataac
      781 ttgtatccac gcaattcttt tagtatattc tcaacagcac cttttgaatc agatttattt
      841 tcaagtaaaa accctcctat agtttcagct attgtaagag tattacctat agcagctttt
      901 ttaacttgag ctccacttaa attatagtca ctaatagaag aacttcctcc catttctaca
      961 gttaaagttc tagctaatct ttctgaccaa attccatcca caggataaag gatggcagaa
     1021 ttaccttttt catcagacat tacggcagga gatgcctcat atccttcaag ataaaaagtt
     1081 accatttgag cttcagggaa agctctaccc atagaatctg catctaagat aggaagtcct
     1141 tttttagcag caactgctat aggcataagt gagtttatcc ctcctatttc tataggaatt
     1201 ataacatcca cttttctatt taataatttc tccattattt ctaaaggggt agttaactca
     1261 acttctgaaa gtaatttttc tattgtaatt acaggagaac ccataccaga tactggaact
     1321 accaaagcat catcatcaag ttcatctaaa cttattactt ttacaggacc atacttttta
     1381 atggcttgtt ttgcaaccaa tttacctaca tatgggtcac ctccacctcc agttccaagc
     1441 actgctgcac caactgctat tttatctaca ctttcttctg ttaaaaatct catgttactt
     1501 tctcctcata tttctaattt attttatact ttataaacaa atttctttat actgctttgt
     1561 ctatttcttg cttatttttt gttgaacaaa gcacttttcc aataatagat tgtactacaa
     1621 accctgctat aaaaccatct aaaggtgcta tagtagtaag agttataaat ccatatgttg
     1681 aaaggtaagt tattaatata ccaattatcc aagacactat tgaacgttta actattggtt
     1741 tatataatac tcctttatca aagcctttaa gttcttgctt tactatgtaa tattctgctg
     1801 tatatacacc accaacaggt gcaattacaa ttccaagtaa atttaaaaat cctataaaac
     1861 cttcatatat tccaaataca gccaataatg ttgctataat accaaggact atagttaata
     1921 ctttctcagg agccttctta agtacaagag ctatacttaa tgatgctgaa tatacattac
     1981 ttgtatttgt agtccattgt gctaatgtaa gtactattat acctggtata gcaattccta
     2041 atgtaataaa aattctcata atatctgaag ttcccataca ttttactaat actatcgaaa
     2101 ctattatcat aaaagcattt ccaaattgta ttcccaaaaa gcttgaaaga gcacagtctc
     2161 ttctactttt agcccaacga cttatatcag gagatacaat tgcaccaact attattatac
     2221 tcattgacag agaaactcca cctgcaaatg tcatagtaga tttcatatta tcatcaactg
     2281 aaattccatt tgctctaaaa gctaagaata tagttagcat cataagaact aataaaaatg
     2341 gtactgaata cacactcaac ttctccattg ccttatatcc ataaattgca gtactcatca
     2401 tcaagagacc acctattagg cttaaaatct gcatacttac atctaaatta aatatatctt
     2461 ttattattat ctgtgcattt tgagcaaaga aacctacttg aactccaaac caacctaata
     2521 gagaaacccc tataactaga gaaaatattt ttgctccagc ttctccaaat acatactttg
     2581 ttatcataga tgtagataaa cttgtagatg agccaaccaa agagcatgta gtatacaaaa
     2641 ttgcagaaaa aaatgaacca acaattactg acaatattgc ttgtttcatt ggcatactct
     2701 gtgccaactc agcgcctaaa ataacagatg ataaatccac acctattgct actaaaactg
     2761 aaaacatagc tacccaacca cgtttttgat tctctggaat ctgttccaga gcatagttaa
     2821 ttttttcttc ttccattttc atccctccat ttaaatcgta aaactactga ccttagcata
     2881 ttttacaatt ttctaattta taaatttatt aactatcata tttttataat tttcatttaa
     2941 tatatagttt taaattttct gaaataagtt tataataaaa aacgcctctt ggaaaaatcc
     3001 aagagacgaa tataaatccg tgttaccact cttattagta aaaattaatt ttactcactc
     3061 attttcttaa ggcgaattaa accactattc taacataatt acaatagtat tctccaaagt
     3121 ccttttcaca taaattccaa tattagttct cagccaaatc taactctctg taaaattcct
     3181 ttatgctact ttcttcttct acgaatatat aatattgatt tgtagttaat ttatgattta
     3241 tatcataata tatatgcaat attttgtcaa cttaaaaagt caattacttg cattttttag
     3301 ttttatatgt aaattttcat
//
LOCUS       DAMJVI010000198         3283 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.204, whole genome shotgun sequence.
ACCESSION   DAMJVI010000198 DAMJVI010000000
VERSION     DAMJVI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.204"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(56..628)
                     /locus_tag="PC545_003643"
     CDS             complement(56..628)
                     /locus_tag="PC545_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDF2458352.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(657..923)
                     /locus_tag="PC545_003644"
     CDS             complement(657..923)
                     /locus_tag="PC545_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HDF2458353.1"
                     /translation="MKDSSRAELMRKVQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(923..1132)
                     /locus_tag="PC545_003645"
     CDS             complement(923..1132)
                     /locus_tag="PC545_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HDF2458354.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            1401..2261
                     /locus_tag="PC545_003646"
     CDS             1401..2261
                     /locus_tag="PC545_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDF2458355.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            2309..>3283
                     /locus_tag="PC545_003647"
     CDS             2309..>3283
                     /locus_tag="PC545_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HDF2458356.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRDLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNVDIQNLLIIRDIIRPPFNDINSNLDNCFNKF
                     KESLYKKEGNYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEIMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDVLEDLMLYRMNEDEYKYEIKNIIKMFDEDLDN"
BASE COUNT         1262 a          377 c          540 g         1104 t
ORIGIN      
        1 aacacaacag taaattataa attatcaaat aataatagtt atacaaataa aaattttaga
       61 acattttttg agattcttct aactcgtaag catccattaa ggcttcacca aatctttgat
      121 aatgaattat ttctctttga cccaagaatt ttaatacatc tataacatca acgtcatctg
      181 ttaggtttat aagttgataa taagttgcaa gtgctttttg ttcagctgcc atatcctcat
      241 gtaaatcggt tacgggattt gacataactg ctataggtct tacatcaaat ggaacaccat
      301 ttgaatctgt tggataaatt ccatatccat tttgagcata gtttgaacca agacctgcag
      361 cttttaactc ttctgggctt gcatcagaag ttaactgata aaccatagta catataagct
      421 caacgtgagc tagttcttct gtaccaatat ctgttaaaag tgcacgcata ttaccagtag
      481 gcattgtata tctttggctt aaatatctta aagatgcagc aagttctcca tttggcccac
      541 caaattgagt tataagaaat ttagccattc tagggtcaca agtttttata ttaactgggt
      601 gttgtatagt tttctgatat atccacatga attttcaatc ctccaatact tgatgtctaa
      661 taattaaatt ctctgtccca aggccaaggt tggtcagccc attgccaagg gcatctactt
      721 ggagcatacc caaagttagt taaaggaccg tatttatttt cgtaagcata tctagcttgc
      781 gcaaattgat tacataatga attataagtg ttgatagcat ttttatcatc agggtgattg
      841 tctaaatata agttcatatc aacacatgca aaagatgttt cctgaacttt tctcataagt
      901 tcagctctag atgagtcttt cattatcttc tacctcctct atattttttt ggcttagcat
      961 ataattcttt gttgtacatt tcagactcta gtaagtttag ttctggaaat atagttccaa
     1021 attttaatgc atcagaaagg cagtacattt ctgtgaaaat ttgattatta atgtatggtc
     1081 tagccaaaga tggaccacaa ttttttttga tatctctagt cttattttcc aagatgaaaa
     1141 cctccttgtt gtaaatagat aggtaactct atctagtgta taatattcaa tcatttaaaa
     1201 atcgttacta ataataaaat attaaatgag aaaaataaaa aagataataa aagaaagagg
     1261 ttatttatgt tattataagt ttatgtgtta ttgtaaacta taaattattt agtaaaatta
     1321 aagtaaaaat tatagtattt tgtggatgaa ggtagacttt tttgtaaaat attattaaca
     1381 taaatatacg aggtgattag atgagtgaac tatcaggaaa aggctgtgaa attatagttc
     1441 cttttgagga aagacttcca gtaagagata tagaaaaaag tataataaaa aaatatagga
     1501 aaaatttatg gtctaagttt atgaaggcaa taagagatta taaattggtt gaagaaggtg
     1561 ataagatagc tgtagcgatt tctggtggaa aagacagtat tcttatggct aaaatgtttc
     1621 aagagttaaa aaaacatgga caagttaatt ttgatgttga atttattgct atggaccctg
     1681 gatatcatgc aaatataaga caattactta tagataattg tgagtatcta aatataccaa
     1741 tacatttatt tgattcaagg atatttgaga tagcagatga aatagcaaaa gattatccat
     1801 gctatatgtg tgcaagaatg aggagaggag cattatattc aaaggcagaa gaattagggt
     1861 gtaacaaact tgcattaggt catcattatg acgatgtaat agagacaaca atgcttaatt
     1921 tattgtgtgc tggaaatttt aaaacaatgc ttcctaaact taattctact aattttgaag
     1981 gtataaaaat tataagacca ctttattata taagagaaga acatattata agatttattc
     2041 aaaatagtgg tatatggcca ttaaattgtg catgtatggt tgctgctaaa aaaacaggta
     2101 ataaaagata tgagataaaa gacttaataa agagcttaga atcaaatttt aaaaatgtag
     2161 aaaaatctat atttaaggcg gcagaaaatg taaatcttga ttcagtactt ggttggcaaa
     2221 aagatgggga gaaacattct ttcttagaaa attttgaata aaaatagtta aaaagaatca
     2281 ctataaacaa ttggaaaggg tgtttatatt gaaaagagta tatatagatt ttgaaatgaa
     2341 tatgccaaat tctaaatcaa aaagagcagt atttaattct gatataattg caatcggagc
     2401 agttatgtat gatgaaaaaa caaaaaatat tgataaattt aaatctttaa taagaccagt
     2461 aagtaatgag gagttgtatc ctcatataca agaattaaca catatatctt ctgaagaatt
     2521 gaagagtgca ccaagttatg aagaggtcat gagaaagttt aaaaaatggc ttggcatatt
     2581 ttctgatatt aaaggaatat atacttttgg taatttggat ttgacatgtt tcaataacac
     2641 agatatgaga agtgctaaaa aaaataacca tccaaggttt gtaaataaca ttagagattt
     2701 atttgtagat ataaaagaaa aatatataaa ttgtggtatg agatgtatga actatatatc
     2761 attgaaaaat ttacttgaat ttgttaactt agagtttagt ggagatgccc atgacccatt
     2821 aaatgatgca tataatttat ttattttgga tgaggcaatc acaaataatg tagatataca
     2881 aaatctttta ataatacgtg acataataag accaccattt aacgatataa attctaattt
     2941 agataattgt tttaacaaat ttaaggagtc attatataaa aaagaaggaa attataatat
     3001 tgtagatttt tctgttgaaa taataaaaac tgtgagaatg tacttattaa caatcataga
     3061 tgtaaatata caaaatttag aaattatgaa agatataagt aaaaaaatgg atactataga
     3121 taaattgaaa gatatagagg aaggctattt ttatttatta gaagatgtgt acttcgatat
     3181 gaaagatgta ctagaagatt taatgcttta tagaatgaat gaagatgagt acaagtatga
     3241 gataaaaaat ataataaaaa tgtttgatga agacttagat aat
//
LOCUS       DAMJVI010000199         3245 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.205, whole genome shotgun sequence.
ACCESSION   DAMJVI010000199 DAMJVI010000000
VERSION     DAMJVI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.205"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     misc_binding    126..336
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            562..1377
                     /locus_tag="PC545_003648"
     CDS             562..1377
                     /locus_tag="PC545_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDF2458357.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDNGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKDVTEGMAAAIKKSDNNKDFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            1405..2082
                     /locus_tag="PC545_003649"
     CDS             1405..2082
                     /locus_tag="PC545_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDF2458358.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            2141..2863
                     /locus_tag="PC545_003650"
     CDS             2141..2863
                     /locus_tag="PC545_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDF2458359.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEILLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         1297 a          331 c          606 g         1011 t
ORIGIN      
        1 aaataatatc aataaatata aacagtagta tttagataat ttacaagtaa aaattattaa
       61 aaaaataaaa aacttattga caaaaatatt ataaggaagt acaattgtat taacaattta
      121 aggaaacaaa tccaatgata gagaaagtag catatatgga tttacagcga gttaggggtt
      181 ggtgtaagcc taacaatgaa gtctatgtga agagagctct ggaggagatt atcagaaact
      241 ttagtatgat aattggttta cccgccttaa gggattgaga ggataagtac ttacttatca
      301 aaaagagtgg taacgcggat ataattcgtc tcttagctgt aaagctaagg gacttttttg
      361 attttaaaat aaaaatatcc atacagctta aaagctaaca gtcacaaatt ttgaaagtaa
      421 tataagtatt tatcaataag agtggtaacg cggtaaagtc gtctcttagt cattaaagac
      481 tgagagactt tttttgtaac tcgaaacatt taagtattaa aatctgaggg aaattataat
      541 taaaagagag gggtagttaa aatgaaagta tttaaaaaat tattaagttt aggattagta
      601 ttaggattga cattatcgct agtaggatgt agtggtggag gagaaaaaac aaagttagaa
      661 caaataaaag acaatggaaa attagttgtt ggtactagtg cagaatttcc tccatttgaa
      721 tttcataagg tagtagatgg aaaagattca ataaaaggat tcgatataat gttggctgaa
      781 gaatttgcaa aagagcttgg tgtaaaggtt gaaattaagg atatgtcatt tgatggatta
      841 ataggtgctt taaatgcaga ccaagttgat attgttcttg caggtatgtc tccaacacca
      901 gagagagaaa agagtgttga tttttcagaa ttatattatt taagtagaaa tgcagttata
      961 gttaaagatg cagatataga caaagtaaaa actgaagatg atttaaagaa acttagagtt
     1021 ggagttcaag caggaagtat ccaagaagaa tatgtagtta acactttaaa aatgacaact
     1081 acaaaatctt tgaaagcaat acctgattta attacagaat taaaaaatgg aaacatagat
     1141 gcagttgtta ctaatgaagc agtttcttta ataaatgtta aaaaatatga tggaataaaa
     1201 atggcaaata ctgaagttgg gaaagatgta acagaaggta tggcagcagc aatcaaaaaa
     1261 tctgacaata acaaagattt tatagaatta ttaaacaaaa agataaaaga attacaagat
     1321 ggcaagaaga tagaagaatt tttaaatgaa gcatctactg aagctgcttc aaattagtaa
     1381 gaaagttatg tgggagatga aattatgagt ttagatttta gttttttaag tagatttggg
     1441 acttcttttt tggaaggaac aggtgtgaca gtatcaattt ctcttgtggc attatgcttt
     1501 ggatttataa taggtataat tatctgtatg gcaaaaatat caaagagcaa agtattaaga
     1561 gcaatatcat caatctatat tgaggtttta agagggacac cattattagt acagatatat
     1621 atagtatggt ttggtttacc tcaattagga ataagattcc ctatgttgtt tggtatacca
     1681 agtgaattta tagctagtgc atttgcatta tcagtaaact ctggtgcata cgtggcagag
     1741 atacttagat caggtataca atcagttgat aatggacaaa tggaagcaag tagatcatta
     1801 ggattaaatt attggtcaac tatgagatat ataataatac ctcaagctat aaaaaatata
     1861 ttaccttcat tagcaaatga gtttattaca cttgtaaaag aatcttcaat aatatctgta
     1921 ataggagttg ttgagataat gcgtactgct gatatagtaa aaaatgcagc atttagagca
     1981 ttagagccat taatagtagc agcagcaatt tactttgtta taacatttac tctatcaaga
     2041 ttagttggat tattagaaaa gaaactatct gtgtctaact agatgagagc atgtttagca
     2101 tttagggaaa acatagaata gtaaaaagga aagtgataat atgattacaa taaaaaattt
     2161 aagtaaatct tttggagatt taaatgtatt aaaaaatata gacttagaaa tcgctaaagg
     2221 cgaaataatg gttatagtag gtccaagtgg ttctggtaaa agtacacttc ttagatgtat
     2281 gaatttacta gaaataccta caggtggaga aataatattt gaaggaaaaa atttggtcga
     2341 taaaaaaact aatattgatg aggttagaca aaatataggt atggtgtttc agaactttaa
     2401 tttattccca cataagacta ttttagataa tataacatta gcaccaatta aattaaaaaa
     2461 gatgactaag gaagaagcag aaaaaaaagc agaaattttg ttgagtagag ttggtctttt
     2521 agataaaaaa gattcatatc catcacaatt atctggagga caaaagcaaa gaatagcaat
     2581 agctagagca cttgctatgg aaccagatat gatgttgttt gatgagccaa catctgctct
     2641 tgacccagaa atggtaaacg aagttttgga tgttataaaa gaacttgcta aagaaggtat
     2701 gacaatggca attgtaactc atgagatggg atttgctaag gaagttgcag atagagttat
     2761 ttttatagat ggaggaagta tacttgaaga taatactcca gaagaagttt tcggaaatcc
     2821 aaaacatgaa agaacaaaag cgtttttagc aaaagtgcta taatatataa aaaggatgtt
     2881 attgatatta gaaattcaat acatcctttt ttagtgatgg ttaatattaa ttttaaaatt
     2941 aaagttatat taatattaac tttattttta cgattttttg cagaaaaaaa aagagctcgg
     3001 ggtaagctct ttttttccac cgaattaatt tccgttacta atccgatttt taataaatat
     3061 aaaatatatc cttatgaaag ggtattggga atagatatat taactaacta gggttaccag
     3121 ggggataacc ttaatttatt ataacaaaat gcgacacaaa aatcaatatt ttttttgaaa
     3181 taaattataa aagttatttt tataacaagt aagcttataa atttaacttt tatagtaaaa
     3241 ataag
//
LOCUS       DAMJVI010000200         3209 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.206, whole genome shotgun sequence.
ACCESSION   DAMJVI010000200 DAMJVI010000000
VERSION     DAMJVI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.206"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            11..622
                     /locus_tag="PC545_003651"
     CDS             11..622
                     /locus_tag="PC545_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDF2458360.1"
                     /translation="MINDIKNKFKNYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            920..1258
                     /locus_tag="PC545_003652"
     CDS             920..1258
                     /locus_tag="PC545_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HDF2458361.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVPFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
     gene            complement(1665..3101)
                     /gene="miaB"
                     /locus_tag="PC545_003653"
     CDS             complement(1665..3101)
                     /gene="miaB"
                     /locus_tag="PC545_003653"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDF2458362.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPDMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPG
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHDRFNRVLEAVNRISAEINDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGG
                     NDDLIGKLVMVKITEPRTFSLNGILVNN"
BASE COUNT         1101 a          474 c          372 g         1262 t
ORIGIN      
        1 ggaggtaaaa atgataaacg atattaaaaa taaattcaaa aattacaaac catatataaa
       61 tggatacaag aatatgaaga gagcatctgt tttaattcca atagtagaaa taaataacac
      121 acactatata ttatttgaag ttcgctcaaa aaatttgaag catcaaccta gtgaaatttc
      181 ttttccaggt ggtaaaatag aatcaggtga gactccatat gaagctgtta ttagagaaac
      241 ttgtgaggaa cttggaactt ttagtgacaa tatagaagta atatcaagtc ttgacctcct
      301 tataacacca gtaaagttta tcatacatcc atacttagga tatataaaaa atattaataa
      361 tttaaatata aacaaggatg aagttgacca tacttttttg gttcctataa aatatctttt
      421 agagaatcct ccaaatactt atagtaatac tgtaaaagtt tttccagacg aaaatctacc
      481 atatggttca attccaaacc aaaaagatta taaatttgaa agtggaagtt atcctattct
      541 gttttataaa tataaagatt atgtaatttg gggaatgaca gcaaaaatac tagaaaactt
      601 tttaaatttt ttatcagaat aacactcata ataaatcatc aaaactttta cataagaaat
      661 ttataaaagt ttctaaattt aacaattcta aaatatcaat aactatattt tgtagcactt
      721 ttttatttta ttattgtttg aatatttgat ggttttcaat gcaatttcaa cataaatact
      781 atgtattagt gtatactgtt gatgaattaa agtatgataa tctatttaat aaagtatgaa
      841 gtaaatgatt ttaatgtgcc tttatggtaa aattactaat aagtaataaa attaattaaa
      901 atatatggag gtgcttataa tgtttgctgt aggatttgtt gttataattg cacttgcctt
      961 tttcatttta agtgtactat tatttaatgg aaaagccagt atgctactag caggttataa
     1021 ctctatgagt aagaaagaaa aagcacgata cgataaaaaa aaattatgta gaagttctag
     1081 cattgttact tttattgtct ctataatgct atttattatg gcgtttttgg gatatagaat
     1141 agagagtggt caaatggacg aaaaaaaaat ggttcccttt tctataatat ttataatagt
     1201 catactttcc agtgtatttt ttaatatcct ctactctaac aaaaaatgta aaaaataaat
     1261 agttgtaagt tgagcagtta attataaaag attgactgct ttttcatgta tattgactac
     1321 tcttattaaa tattttaaca tttaaaaatc tatgtaaaaa taatgaatat agtacaaact
     1381 tctaataatg attttacctt tgtggttaca gtccactata tataatttaa tatgataaca
     1441 gggaattact aatgacaact aggtaagctt ttataaaata taataaaaca aaaatagatg
     1501 tacaatgaaa ttatatctta tatcaaattt atttttatta atagcaaaaa cttacattag
     1561 cataacatat gacaaattcg cttaaaataa aaatttttaa tataatttgt tctatagtac
     1621 atctattttg attagtttac ataattttat tattttgtac aatattaatt atttactaat
     1681 atcccattta atgaaaaagt tctaggttca gtaattttta ccataactaa tttaccaatt
     1741 aaatcatcat ttccaccttc gaaatttacg agtttatttt gtctagttct accagcaaac
     1801 ttattttcat tatttttact tcttccttca actagaactt caactattct atctttatat
     1861 ccatcattta tttctgcaga aatcctattt acagcttcta acaccctatt aaatctatca
     1921 tgctttatat cttctggtat ttgattttcc attttagcag caggagttcc ctgtctttta
     1981 gaataaataa atgtaaaagc tgaatcatat ctaacttttt caaccacatc taaagtgtcc
     2041 aataaatctt cttctgtttc tcctgggaaa ccaatcatta agtctgtaga aaaagcaata
     2101 cctggaactt cctttttagc tttttctatt atttctaaat aatattcctt tgtataatgt
     2161 ctattcattt ttttcaaaag gctactgctt ccacattgta ttggtagatg taaaaactca
     2221 cacaccttat cacaatctct aattgcgtat attacttcat cagatatatc ttttggatga
     2281 gatgtcataa atcgaaccct ttcaattcct tctatatcat tcacttttct taaaagttct
     2341 gagaatgaaa ctggattttc aagtgtctta ccataagaat ctacattttg tccaagtaaa
     2401 gtgatttctt tcgttccatt tgcaacaagc tcttttatct cgttaattat atcttcagga
     2461 gttctacttc tttctcttcc tcttgtgtat gggactatac aatatgtgca gaaattattg
     2521 catccataca ttatatttac aaatgctttt aattcaaatt ttctattact tctaagacct
     2581 tctattactt ctccatccac atcccataca tccactaaca ttttgtcaga atttattgat
     2641 tctgtaagta attgcggaaa tttatataga ttatgtgttc caaatataag gtctacatgt
     2701 ttatattttt ttcttaattc ctctacaaca tgtggttgtt gcatcataca tccacataca
     2761 gctattttca tatctggatt ttttcctttt agatgtttta attgtcctaa gttaccatac
     2821 acttttagct ctgcattttc tctaactgca caggtgttat atattattaa atcactttct
     2881 tcaaccatca ttgacatttg gtatcccatc tcttctaaca ttgaacataa tttttctgaa
     2941 tcatgttcat tcatttgaca cccgaaggtt tgagtaaaca cgagttttct ctttccagtt
     3001 ctgttgaagt attcttcatt ttgtctgtgt atttgttcta tatatttatc ttgttcttgt
     3061 attttctcta taggtattgt tacttgtttt cttttgctca cgtctattcc tcctagttaa
     3121 tttcttatat tacacataac cttaattata tcaaataaga aaaatctttt aaagatatta
     3181 ttatttttct agttgtgttt taatagtaa
//
LOCUS       DAMJVI010000201         3152 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.207, whole genome shotgun sequence.
ACCESSION   DAMJVI010000201 DAMJVI010000000
VERSION     DAMJVI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.207"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(102..659)
                     /locus_tag="PC545_003654"
     CDS             complement(102..659)
                     /locus_tag="PC545_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458363.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKVFLVIFLFMFSMGVASLWEIGEFLMDTFVGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSNI"
     gene            complement(687..2213)
                     /locus_tag="PC545_003655"
     CDS             complement(687..2213)
                     /locus_tag="PC545_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2458364.1"
                     /translation="MNESYWFLNSSPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKDIKDEYRTCKKIGINCEYHETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMVAVLENKLADGMFISIDDPSITFRQY
                     NDGSENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKEVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            complement(2593..>3152)
                     /locus_tag="PC545_003656"
     CDS             complement(2593..>3152)
                     /locus_tag="PC545_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2458365.1"
                     /translation="ITSLYIFKNFKIGILLFFYFLFSQIIFGLIISDNQFDYKNFLNY
                     ILLPIISLVYIYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLY
                     SPQTPIIGAIANLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIIN
                     YHSIIQRLIFILLFYTPFLFTKEYK"
BASE COUNT         1148 a          526 c          275 g         1203 t
ORIGIN      
        1 taataattat aaataacaaa aatggattta attaatcata tagaccttga aactcaacat
       61 gattaattaa atccattata attaatataa aaaatttaaa actatatatt agattttttt
      121 actataaaag gaatagttat aattgtgcct attaaagcat ctaccatatc aatcatagta
      181 tcttctaacc ctccggcctg tgtagtagtc ccaacaaaag tatccattaa aaattcacct
      241 atttcccata aactcgctac tcccatacta aacataaata aaaatataac taaaaacact
      301 ttactcattt tacttatctc tctttgtgta taaaagtatt taattataga aaaagccaca
      361 atacaagata ttatacctga ccataaatgc aaaaaatcat cataataatt tatatcataa
      421 aaattaaaac aagtacctaa caatgatgaa aacattataa ataaagcaag cattatatat
      481 aattctttac tcaataatct ttccgtttgc ttatttataa aactcaaaat gaaagtacca
      541 actaaactaa ttgctaccag acctagactt ccatattctt tgtttgctac aaaaaatacg
      601 gcagataaaa tataaactat atacaatagt atagttattg gctttaaaag tttattcata
      661 tacactttac cttcctaaaa ataattttat aataacttag gattaacatc attatcacca
      721 tgtccataaa gttttaatgg cttaagagct ggtccttcaa gtatatttcc cttatagtca
      781 aatctagaac catgacatgg acagtcccat gttttttctt cactgttgaa attaagttca
      841 catcctaaat gagtacatgt agtgtctaca atataaaaat ctccattatc atccttatat
      901 acaccatatc tttttccatc aatattaaca atctttcctt ctccattatt tttaggcata
      961 tctccactac ctatttttaa ttttccactt acataattta ttgctacgtt tgcattttcc
     1021 tttataaaat cttttgaaaa gtagctacct aatctggatg gattaaaagt atctttataa
     1081 tcagaattat tatttattat caaatctttt attattattc cagcagcagt tccattagta
     1141 attccccatt tagagaaccc tgtagctaca taaatattgt cttctctttt gttgatataa
     1201 ccaatataag gaacattgtc aaaactcata ttgtcttgag cagaccattt ccccttaaaa
     1261 tcttttacct taaagacttc tttaccataa tgctctaaat catcaaatat ttctttctca
     1321 gttcctccct gaccaacttt atggtctcct cctccaaaaa tcagtaaatt ttcacttcca
     1381 tcattatatt gtctaaaagt tattgatggg tcatctatac ttataaacat accatctgct
     1441 aatttatttt ccaaaactgc aaccattaaa taagcacgct ctgcatattc ttttgcaaaa
     1501 taaaaattca atccatcata ccatggacta tgagatgaaa ttataacatt ctcagcttct
     1561 attatattat cctctcttgt tttaacccta catatttttc ctttttcaag gtctactact
     1621 ggtgtatttt catatatttt tagccctaaa tttactgctg cttttgccaa tccatcaata
     1681 tacttctttg ggttaaattg accttgatta gtaaaactaa tagccccctt tatatctaat
     1741 ggaatatttt ctatagtttc atgatactca caatttattc ctattttttt acatgtacga
     1801 tattcatctt taatatcctt tatagtatcc tctttttctg taaatacaaa agaagtttcc
     1861 cttttaaaat cacattttat attgttttct tctattattt gttcaactaa atttaaagct
     1921 tcattatttg cattataata tgattttgct ttatctaatc catatttttt ctctatctta
     1981 gagtaaaata tatcatgctg acaagttacc ttaccagtat tccttccaga agttccatat
     2041 cctattttat cagcctcaac aagcactact tttttacctt cttttgctaa taaatatgca
     2101 gttgtcaatc cagttattcc accacctact ataagacaat ctgtttttat attttcacct
     2161 aatttgttat attcttttgg acttgagttt aaaaaccaat atgattcatt catttaaaat
     2221 actcctttac atattaaata gtaatagttt tcccatcatg gagtttattt attataaata
     2281 gtataaaaaa taagcttaat aaaaagttaa attaaaaatc acttagtttt cttgttttat
     2341 acaccagact aaacttatat atatttataa tttctatatt attttcttaa ttaatagtat
     2401 ttatgctaag taaaaatgag gatattttag atttcaatac ataagatgat gttgtgctaa
     2461 caattataaa ttgttatcca ccaacttttt tattgattta gttagtttaa tatttttctg
     2521 atatgtaata taacaatctc tctataaaaa tatttactta aaaatttatt ttatataaat
     2581 taattgtaat tcttacttat attcttttgt aaatagaaaa ggagtataaa ataatagtat
     2641 aaatatcaat ctttgtataa tactatggta attaataatt atttcagaat cattaaaaat
     2701 taatcctaag ctattaatta gaacatgcat tatagagcaa aaccatatgt tcttagtttt
     2761 catataaaca taacctaaaa atatagaaag tccaatacca tatatcaaat tggctatagc
     2821 tccaattatt ggagtttgag gactatatag tgtaaattgt aaaggtaagt gccataaact
     2881 ccaacaagtg cctaccatta caatccctat ttttttgcca aatctatcaa ataaaatact
     2941 ttgcaaaaaa gatctccaac catactcttc acataaaaaa ggataaatgt atactaatga
     3001 tattatagga agaagaatat aattcaagaa attcttataa tcaaattgat tatcacttat
     3061 tattaatcca aaaataattt gagaaaacaa aaagtaaaaa aatagcaaaa tacctatctt
     3121 aaaattttta aaaatatata aacttgttat tt
//
LOCUS       DAMJVI010000202         3144 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.208, whole genome shotgun sequence.
ACCESSION   DAMJVI010000202 DAMJVI010000000
VERSION     DAMJVI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.208"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            85..2442
                     /locus_tag="PC545_003657"
     CDS             85..2442
                     /locus_tag="PC545_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HDF2458366.1"
                     /translation="MDKVNLERYSCDINVGLNKNQLQSRIDDNLINAFDNGKTKTYKQ
                     IFKDNIFTLFNLINIVLVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIREKEKKEIDVQELVLDDIMILKTGNQICADSKVLNGKLEVNESLVTGE
                     SDIIIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKIVGIIIIPLGIILFAKQHFGNGDSIATSVVGTVAAVNGMIPEGLTLLTSIALAV
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDIVMLEEVDINEIMG
                     NICNSLKDDNATLNAIRDKYNVLDTYKAKNLIPFSSERKYSGVTFENKGTYLLGAFEF
                     IFKEKNKKLRIEVERYSKKGNRVIVLAHSDSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPITVSEVARKAGLKTASNFIDATTLKNDSDIYKAVDK
                     YSVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRASSLFLVKTIFSFFLSLLTLFFFSRQYPF
                     VPIQLSLIGAVTVGIPSFFLALEANKSRISGNFLLNILKNALPGALCVTVNVIIVYNI
                     VEYLGYSSSVFSTMCAILTGVCGLLILRQQCLPFNKERLFLIVSMTIAFVIGILFLGG
                     LFSFVALSRELILITMALAILMPIMIKVMLFVLDEILETIVPDLN"
     gene            complement(2528..2845)
                     /locus_tag="PC545_003658"
     CDS             complement(2528..2845)
                     /locus_tag="PC545_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDF2458367.1"
                     /translation="MSEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEMLAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
BASE COUNT         1221 a          336 c          505 g         1082 t
ORIGIN      
        1 aataataaaa taaaaaaatt gagaatttta taatatattt gtagatttga gtataaaaaa
       61 gtgctaagtt gaaaggagta aaatatggat aaggttaatt tagaaaggta tagttgtgat
      121 ataaatgttg gtttaaataa aaatcaactt caaagtagaa tagatgataa tttaattaat
      181 gcatttgata atggaaaaac caaaacatat aaacaaattt ttaaagacaa tatatttaca
      241 ctttttaatc tcataaatat tgttttagtc tgtttattag tttcagttgg ttcttttaaa
      301 aataccttat ttatatttat tgctgtaatt aatactatta taggagtaat acaagagata
      361 agagctaaaa gattattgga caatctatct gttatagttt caaataaggt atctgtcata
      421 agagaaaaag agaaaaaaga gattgatgta caagaattag tattagatga tattatgatt
      481 ttaaaaacag gtaatcaaat ttgtgcagat tcaaaagttt taaatggaaa attagaggta
      541 aatgagtctt tggttacagg tgaatctgat attattataa aaaataaagg cgatttttta
      601 tattctggaa gttttgtagt ttctggagaa gcttttgtaa gagttgaaaa tataggaaaa
      661 gataactatg caaataagat agctaatgat gcaaagattt ctaaaaaaca taattctcaa
      721 cttagaaatt cattaaatgc aatattaaaa attgtaggta taataataat tcctttagga
      781 attatattgt ttgcaaaaca acattttgga aatggagatt ctatagctac atctgtagta
      841 ggtacagtgg cagctgtaaa tggtatgata ccagagggat taacattact tacgagcatt
      901 gctctagctg taggaactat aaaacttgct agtcataaaa ctttagtgca agaactgtac
      961 tgtgtagaga ctcttgctag agttgataca ctatgtcttg ataagacagg tacaattaca
     1021 gaaggtaaaa tgcaagttga tgatatagta atgttggaag aagtggatat taatgaaata
     1081 atggggaata tatgtaattc tctcaaagat gataatgcaa cactaaatgc aataagagat
     1141 aagtataatg ttttagatac gtataaagct aagaatttaa taccattttc ttcagaaaga
     1201 aaatacagtg gggttacctt tgaaaataaa ggtacatatt tattaggtgc atttgagttt
     1261 atatttaagg aaaaaaataa aaaacttagg atagaagttg aaagatattc taaaaaagga
     1321 aacagagtaa tagttttagc tcatagtgat tcttatatgg aagataataa tattcctgaa
     1381 gatataaagc cactagcttt tatattgata ttggataaaa ttagagatga agctaaggaa
     1441 actttagaat ttttttacaa tgaaggtgta gatataaaaa ttatatctgg tgacaatcct
     1501 ataacagtaa gtgaggtagc tagaaaagca ggtcttaaaa cagctagtaa ttttattgat
     1561 gcaacaacac ttaaaaatga tagtgatata tacaaagcag ttgataaata cagtgttttt
     1621 ggaagggtaa gcccacaaca gaaaaaacaa atgattttag cattaaaaaa acaaaatcat
     1681 acagtagcta tgactggaga tggtgtaaat gatgtactag cacttaaaga agcggattgt
     1741 agcattgcca tggcatctgg aagtgatgtt actaagaatg tgtctaattt agtcttatta
     1801 gattcaaact ttgcatcaat gccaaaggtt gttatggagg gaagaaaagt aattaataac
     1861 attcaaagag catcttcttt gtttttagta aagacaattt tttcattctt cttgtcgtta
     1921 ttaacattat tcttcttcag tagacagtat ccatttgttc ctatacaatt atcactcatt
     1981 ggggctgtaa cagtgggtat accatcattc ttcttagctc ttgaagcaaa caaaagtaga
     2041 atttcaggaa attttttact caatatatta aaaaatgctt taccaggagc actatgtgtg
     2101 acagtaaatg taattattgt ttacaatata gttgaatatt taggttatag ttcaagtgta
     2161 ttttccacaa tgtgtgctat attaactggt gtatgtggtc ttttaatatt gagacaacaa
     2221 tgtttgccat ttaataaaga aagattattt ttaatagtaa gtatgactat tgcatttgtt
     2281 attgggattc tatttttagg tggattattc tcatttgtag cattgagtag agaattaatt
     2341 ctcataacta tggcattagc aatattaatg ccaattatga taaaagttat gttatttgtc
     2401 ttagatgaga tattagagac aattgttcca gatttaaatt aattttaaaa tgatataaat
     2461 aagagcagtt ttagttgaac tgctcttttt agatgtgttt aattttatgt aagaatatgt
     2521 atttttatta atcagctata tatgagcaac agtagtcaac aacttcatta acctttaagt
     2581 caaaacttga atcactagga acattaaatt tttgcccttc tttataagtt acaaactcat
     2641 tgcttccagg cagttttaca tccatacttc cagcaagcat ctccattatt tcttcttctc
     2701 tagtagaaaa agtgtattct cctggcaaca ttaatcctaa agtttttctt tctccattag
     2761 gcagtattat tactctgcta cttaccttac catcaaaata aacgtttgcc ttctttactg
     2821 cagtaacatt cttaaattca ctcattagat aaaacctcca aaattatttg tacatatata
     2881 ttatataaaa ataatgtaaa aaatgctata tatttacaaa aaaatttagt atttattaaa
     2941 aaaattaata agaataaaaa agaggtgaaa ttgcttaaat tgataaaaaa tataaaataa
     3001 attgcaaagt aagcaagaaa agaataagtt gaagagttat atattataaa tattaaaaat
     3061 tttagtatta ataaaaaaga tattacatta atatgaaaat ataggagatt agaagtgttt
     3121 gattaaaaaa tagctacaca cact
//
LOCUS       DAMJVI010000203         3135 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.209, whole genome shotgun sequence.
ACCESSION   DAMJVI010000203 DAMJVI010000000
VERSION     DAMJVI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.209"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..1077
                     /locus_tag="PC545_003659"
     CDS             <1..1077
                     /locus_tag="PC545_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2458368.1"
                     /translation="NLKIIIKKKKDICDYLNKISNYKKVKSTIEKLETRGKMPKWYLE
                     LINKVKNILENMLYNRNLSDEQIFYLNLFLDIQNLFLGIYELKSESVIFENTLDDNKI
                     IAKMDTYIIIDYINASFEYLGYVLNGSALNSIDYGVPQNRERFVLIGAKKDFLKNKKI
                     ETPKPIVGENYVTVRDAIGDLAEYEASKGSMDYCFEKNSNNIEKDFYRKIILDSNKIY
                     NHVCTDTRNIALKRFEYINQGNNFHSLPDELKGTYADPERTQNTIYKRLVYDKPSDTV
                     VNVRKSMWIHPIKNRAVSAREAARLQSFPDSYKFLGTKDSVYQQIGNAVPPLLGRVVA
                     EKILNLFNDEPNEYLRDVFEALDG"
     gene            complement(2077..2649)
                     /locus_tag="PC545_003660"
     CDS             complement(2077..2649)
                     /locus_tag="PC545_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HDF2458369.1"
                     /translation="MEKLVKILINIICIIALTFSSLSIYIKLSEYKKADEVYTELREN
                     TINNSKHQELYDKNNDYRFWLKINNVNINYPVVQGYNNDFYLTHDFYKNYLPLGSIFM
                     DYRNNFENDKSLIVYGHYMKNKTMFGQLENYTDEVFFKENNLVEINYKVQTYTYEIFS
                     VYTADLINRDYLSIHFNNNDEFKYSLNYIT"
     gene            2918..>3135
                     /locus_tag="PC545_003661"
     CDS             2918..>3135
                     /locus_tag="PC545_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458370.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNASNTFADTIEESTNAGN
                     AEGDTNAGNAEGDTNAGNAEGDTNAGNAG"
BASE COUNT         1223 a          349 c          442 g         1121 t
ORIGIN      
        1 aatttgaaaa taattattaa gaaaaaaaag gatatatgtg attatttaaa taaaatatct
       61 aattataaga aagtaaaatc taccatagaa aaattggaaa caaggggaaa aatgccaaaa
      121 tggtatttag aattaataaa taaagtaaaa aatatattag aaaatatgtt atataataga
      181 aatttatcag atgaacaaat attttatttg aacttattct tggatattca aaatttattt
      241 ttaggaatat atgagttaaa atctgaatct gtaattttcg aaaatacact tgatgataat
      301 aaaattatag ctaaaatgga tacttacatt ataatagatt atataaatgc atcctttgag
      361 tatctaggat atgtattaaa tggaagtgca ctaaattcaa ttgattatgg agtacctcaa
      421 aatagagaaa gatttgtatt gataggagca aaaaaagact tcttaaaaaa taaaaagata
      481 gaaactccta agcctatagt tggagaaaat tatgttacag taagagatgc aataggcgat
      541 ttagctgaat atgaggcttc taaaggtagt atggattatt gttttgaaaa aaatagtaat
      601 aatattgaaa aagattttta tagaaagata atattggata gcaataaaat atacaatcat
      661 gtatgtacag atacaagaaa tatcgcacta aaaagatttg aatatataaa tcaaggaaat
      721 aattttcatt ctttgccaga tgagttaaaa ggaacttatg ctgatccaga aagaactcaa
      781 aatactattt ataaaagact agtatatgat aagccatcag atactgttgt aaatgtaaga
      841 aaatctatgt ggatacatcc tattaaaaac agagctgtta gcgcaagaga ggcggctaga
      901 ttgcagtcat tccctgatag ttataagttt ttaggtacaa aagattctgt atatcaacag
      961 ataggaaatg cagtaccacc tttattaggt cgagtagtag cagaaaagat attaaattta
     1021 tttaatgatg aaccaaatga gtatcttaga gatgtgtttg aagcactaga tggatagttc
     1081 aaataaaata gtgggtatta ttcttgtgat tcttatccaa cattaatcca cgaaataagg
     1141 actgtcgaat gagtaatttt aaattgctgt gcgacagttc tttttttact tgcttttcat
     1201 taatttatat ttatgcaaaa tttgagcttg aaaacggtca taagaaataa aggtataaaa
     1261 atacaatttt aaaatagaat aatttacaaa gtgtaagatt aaaaaatatt tttgagagga
     1321 tgaatataca tactacattt attaatgatt atcttatttg tgaaacttag aaatactata
     1381 ggctaacact acaggtataa actctgaaag aatattttga ttgcctttga ttagaattct
     1441 tttaaagttc atactatgtt ttatttgggt gaaagctcct tatgcctgaa tacttctatt
     1501 cattctaagt tcacatcctt gtgtaccagt gattctttct aaattatctt aatgccaaaa
     1561 tcttgttcgc atttcaaaat aaagtcaggt agctacttca acattttagt tatatttctt
     1621 caatagtttg tctccaaaca aaggtacatc tattagaaac agatttaatt ttatttatat
     1681 caataaataa tatatacttt gaaacttcat taatatcacc aagataattt ataagttaag
     1741 ctattatata ctatcttcac caactacatc ctccatagtt ttacactttc ttaattcagc
     1801 ttggtaatct aaatctgtaa taattatttc tctttgattt ttcatttctt tgcctacaaa
     1861 agtttaatat aaatattatt ccatacttac caattttacg ttcaaaaaag actgtagata
     1921 agtctgcatt tacacaaacc tacttatagg tctctttttt gaaattaatt tagcatgaac
     1981 tatagtaaga gcatctttaa attcatagtt acaagtatac aaagttataa ttttatctga
     2041 aatatctacc tttatatcag atttgtgtac tgatttttag gttatataat tcaaagaata
     2101 tttaaactca tcattattat taaagtgtat acttaaataa tctctgttaa ttaagtcagc
     2161 agtatatact gaaaaaattt cataagtata agtttgtact ttataattta tttctactaa
     2221 gttattttct ttgaaaaata cttcatcagt atagttttct agttgaccaa acatagtttt
     2281 atttttcata taatgaccat agactattaa actcttatca ttttcaaaat tatttctata
     2341 atccatgaaa atactaccta aaggaagata atttttataa aaatcatgag ttaggtagaa
     2401 atcattgtta tatccttgta ctacaggata gtttatattt acgttgttta ttttaagcca
     2461 gaatctataa tcattatttt tatcataaag ttcttgatgt ttgctattat tgatagtgtt
     2521 ttctctgagt tccgtatata cttcatcagc ttttttatat tcagataatt ttatgtaaat
     2581 cgaaaggcta gagaaagtta gcgcaataat gcaaataata tttattaata tttttactag
     2641 tttttcaatt aattacccct tagtattatt atataaaaaa ttaaataaat tttatataat
     2701 taaaattttt attgtagtta tttagcaaat aatgaatact tataatatat aaggttgaaa
     2761 aaaacagtat attcattgac aaatataagt tttaagtata tattaaaaat tattagagtg
     2821 catataagga tgtatacagt gtatattaag aattattaga gtgtatataa ggatgtatac
     2881 agtgtatatt aaaaattatt aaggggaggg ggaatttatg caacaaaaat gtaaaaagaa
     2941 agcactatca atgattttaa gtatctcaat gggacttgga tcagtagcat catttaatgc
     3001 gtcaaatact ttcgcagata ctatagaaga aagtactaat gcaggaaatg cagaaggaga
     3061 tactaatgca ggaaatgcag aaggagatac taatgcagga aatgcagaag gagatactaa
     3121 tgcaggaaat gcagg
//
LOCUS       DAMJVI010000204         3057 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.210, whole genome shotgun sequence.
ACCESSION   DAMJVI010000204 DAMJVI010000000
VERSION     DAMJVI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.210"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            379..1656
                     /gene="hflX"
                     /locus_tag="PC545_003662"
     CDS             379..1656
                     /gene="hflX"
                     /locus_tag="PC545_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDF2458371.1"
                     /translation="MDMAKKGITVGLNINNKSEDFNELMIELENLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEEILNLIKKENIEIVIFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLIGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITSLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIVLANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKSHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVDVL
                     DRILDSIDKEGIFVSVKKDINIDKMIKCICDSIFKDYVRCKFLIPYDKGHVVSYFNEN
                     MSIINTEYREDGAILDVECSNIEYNKYKKYALE"
     gene            1889..2386
                     /locus_tag="PC545_003663"
     CDS             1889..2386
                     /locus_tag="PC545_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDF2458372.1"
                     /translation="MDAETLMKKYMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKDRVQEYSKKVRGMQEKVLQLLFEDFTEKEVEQLFFGIMKLSIGIENVKNYVEN
                     NDVGE"
     gene            2436..2960
                     /locus_tag="PC545_003664"
     CDS             2436..2960
                     /locus_tag="PC545_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDF2458373.1"
                     /translation="MKNVVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFLKKNYKELQKKNLVVFGVGAAPYDDEVIKKLREVNFKDE
                     LNKIPCFYCRGAWDETKMSFIDYTLCSFFKKSIAKKERQGLKPAEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
BASE COUNT         1272 a          267 c          533 g          985 t
ORIGIN      
        1 atcatgcaaa taactactta aatttaagaa tagggttaaa tataaaatga taattacata
       61 taagttaatt aaactaatgt atttttaata tatatttaga attttaatcc ttcttatgat
      121 ataagtagct tgttttgtat tacttagatt tgtaaggagt ttttttattg aaaaaactgc
      181 ataatatatt tgggaggagg tgtaagaaag tgatttttta tataagattg agaaaataat
      241 gcacgattat ggttattata atagtaaata agaagtgtca tttaaggata agataaaaag
      301 caaaattctt gatgaattta attatagagc cataatctcg tgctatagat taaataaaaa
      361 atagcagata aggagaatat ggatatggcg aaaaaaggaa taacagtagg attaaacata
      421 aataataaat ctgaggactt taatgaacta atgattgaac ttgaaaactt atgttcagca
      481 tgtgatattg atgttgtagg gagtataact caaaatgcaa agcaggttaa tagagcattt
      541 tacattggga caggtaaagt tgaggaaata ttaaacttaa taaagaaaga aaatatagag
      601 atagttatat tttacaatga actttctaca tcacaactaa aaaatcttga agaaaagctt
      661 aattgtgaaa ttatagatag aacggcattg atacttgata tatttgcaca aagggcaaaa
      721 actagagaag ctaaacttca agtagaagta gcaagtttaa aatatatgtt accaagactt
      781 ataggttcaa atgaaaattt aggacgtcaa agtggtggag ttggtactaa aaatagaggt
      841 tctggtgaga aaaaattaga acttgaccgt agaagaatag aagaaaagat aacatcttta
      901 aatagggagt tggatgattt aaaattccaa agagaaactc aaagaagtat gagaagaaaa
      961 tcaaatcttc caaatgtagc tctagtcggg tatacaaatg ctggtaaatc ttcaataatg
     1021 aataaactag tagatatatt taaaaactct gaagaaaaga aagtgtttga gaaaaacatg
     1081 ttatttgcta cacttgaaac ttctgtcaga aatatagttc ttgcaaataa caaagaattt
     1141 ttattatctg atactgttgg ttttgtaagt aatctaccac atgacttagt aaaagcattt
     1201 aggtcaacat tagaagaagc atgtgaggca gacgtactgt tacatgttat tgatatttca
     1261 aatccaagct ataaaagtca tattaaagtg acagaagata ctttaaaaca aataggtgca
     1321 gatggtatac ctatgataca tgtgtataat aaaattgatt taatagatgt agacgtattg
     1381 gatagaattt tagatagtat tgataaagaa ggtatatttg tttcagttaa aaaagatata
     1441 aatatagata aaatgataaa atgtatttgt gatagcatat ttaaagatta tgtaagatgt
     1501 aagttcttaa ttccatatga taaaggtcat gtagtttctt attttaacga aaatatgagc
     1561 attataaata cagaatacag agaagatgga gctattttag atgtagaatg tagcaatata
     1621 gaatataata agtataaaaa gtatgcttta gagtaaattt tgaaaaactt attatagcct
     1681 aaaatatatt taatttataa tttattataa attgattgta aatatgattt tatgaaacgg
     1741 tatagccata atgctatgcc gttttgccat ttataataaa aataaattta ttgacataat
     1801 agtaactaat tactataata taaagtaatt agttactatt taaaattatc aaaaaattaa
     1861 aatctaagat aaagagaagt gtataataat ggatgcagaa actttaatga aaaaatatat
     1921 ggtagatgat gatagtagaa aacaagctat atttgcaagt atatttgtaa tgcaaaataa
     1981 acttcaaact acatgtgata aattagaccc agaattaaca atgaaacaat ggttactttt
     2041 agctattgcg gactctgtag atgaaactct tacattaacc aaacttggag aattaatggg
     2101 gtgctcaaga caaaatgtaa aaaaactagc tattgcttta gaaaagaaag gttttataca
     2161 aattgagcag aatgaaaaag attcaagagc tatctgtctt gtgacaaaag atagagtgca
     2221 agagtattct aaaaaagtaa gaggtatgca ggaaaaagta ttacagcttt tatttgaaga
     2281 ttttacagag aaagaagtgg aacagttatt ttttggaatt atgaaattaa gtataggaat
     2341 tgaaaatgtg aaaaattatg tagaaaataa tgacgttgga gaataagcaa gtatcaaatc
     2401 aactttgtat gacatcagag agggggataa tttatatgaa aaatgtagta gtgatttata
     2461 agagtaagta tggttcaaca aaaaaatatg ctaaatggct ttcagaatta ttgtcttgtg
     2521 atatttttga aactaagaat attaatatag aaaaggttat gaagtatgat acgattattt
     2581 taggaggagg tctttatgct tgtaatatag cgggaatttc tttcttaaaa aaaaattata
     2641 aagaattgca gaaaaaaaat ttagtagtat ttggcgtagg agctgctcca tatgatgatg
     2701 aagtaataaa gaaattaaga gaagtaaatt ttaaggatga attaaacaaa attccttgct
     2761 tttattgtcg tggggcatgg gatgagacaa aaatgtcttt tatagattat actctttgta
     2821 gtttttttaa aaaatctata gctaaaaaag aacgtcaagg tcttaaacca gcagaagcac
     2881 ctttattgtc tggagaagca tttgattggt gtaataaaga aaatttaact ccaatacttg
     2941 attatataag agaactttaa gaggtatata tttataatta tttattatct aaagttagat
     3001 ttcatagaaa cttactagac attatttata aaataatatt attaaagatg gataatt
//
LOCUS       DAMJVI010000205         3048 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.211, whole genome shotgun sequence.
ACCESSION   DAMJVI010000205 DAMJVI010000000
VERSION     DAMJVI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.211"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     repeat_region   131..1148
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=197..225
                     /rpt_unit_seq="atttacataccacatagttaatataaaac"
     gene            complement(1344..1583)
                     /locus_tag="PC545_003665"
     CDS             complement(1344..1583)
                     /locus_tag="PC545_003665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458374.1"
                     /translation="MECYLKRTLIIFSEAFLSISFKTRKYLFIVIFILVCPNLSEMIF
                     ILVPARRRIIRIDNIVSIYKYVGVLKLINYSILIL"
     gene            1795..>3048
                     /locus_tag="PC545_003666"
     CDS             1795..>3048
                     /locus_tag="PC545_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDF2458375.1"
                     /translation="MLKVLKGNIIFTKTSETFTVFENSYIILEKGKVKGIFKEIPKEY
                     KNLKVVDYTDKLIIPGMNDLHAHASQFKNLGMAMDKELLPWLETYTFPEESNYKDIEY
                     ATKMYKKFVKELWKSGTTRIAVFATVHKEASMKLMDIFKEVGLGAYVGKVNMNMNCPD
                     TLLENTQDSITDTEEIINKYSDTDSIVKPIITPRFIPSCTSELLKGLGDLCLKYNIPI
                     QSHLSENYDEIEWVRSLEPESKFYGDAYNRYNLFGQTPTLMAHCVHCSQDEIDLMREN
                     NVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHTLSIFKAMVSAIQLSKL
                     YWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFDALIIDDSNLNHDNYSI
                     LERLERFIYVGDDRNIIHRYVCGKLI"
BASE COUNT         1200 a          408 c          390 g         1050 t
ORIGIN      
        1 tattttaatc attaaaacta aagtcaatgt tatctggtaa ttatttacta caaatgctaa
       61 aaaataatta tattttaaag gataaaatgc tgtataaatt attggggtat tttacctata
      121 cttgttgcaa atttacatac cacgtagtta atataaaaca agatgttttt aaaaataaac
      181 ttaatataga tattgaattt acataccaca tagttaatat aaaactttaa tcatatatca
      241 gataggtcac aacttaagag tgatttacat accacatagt taatataaaa caacttctca
      301 tattccagat aattgttatt ttacgaaatt tacataccac atagttaata taaaacctgt
      361 agatttgcct gtttcgatat tgctcatgtg tgaatttaca taccacatag ttaatataaa
      421 actctcttgt ttcttctgtt aattttatta taccttcatt atttacatac cacatagtta
      481 atataaaacg cgttatattg ttgtataaaa ccgattaaag ttcaatttac ataccacata
      541 gttaatataa aacttgcaac atattgtttt atcgttgctg ggtctttaaa accatttaca
      601 taccacatag ttaatataaa actaagcgat attagagagc gcaaggcata gcggagcaaa
      661 tttacatacc acatagttaa tataaaactt taattttaac cacccccttt cactaataaa
      721 aaaagattta cataccacat agttaatata aaacttctat taagtgtaag caaaacgctt
      781 ggaatatgaa atttacatac cacatagtta atataaaact tatacacaaa tacaacttga
      841 agaagcgcta aagagattta cataccacat agttaatata aaacctaatg agtacataga
      901 taaacgtaca ggagaggtta atttacatac cacatagtta atataaaacg ttgataattt
      961 tcgtttgtat actttttata atttaaaatt tacataccac atagttaata taaaacataa
     1021 agatttagaa aaagaaaaag ttaaaatttt ggaatttaca taccacatag ttaatataaa
     1081 acttttcctg ttaagttgat gtttgcaact ttaactgcta tttacatacc acatagttaa
     1141 tataaaaccc caaaataaac taagtatttt cagtacttac acacatacaa ctctctcaaa
     1201 tttgcagtga accatgagta gtgcaattga taacatttat cacacaccct caatccctta
     1261 cattgcaact gttaaaccct actttatcaa aaatatcgct cactgcaaaa tttatatatt
     1321 tttattatat cataaaattc aaactataaa attaaaatgg aataatttat caatttaaga
     1381 acacctacat atttgtaaat acttactata ttatctattc taataattct tcttctagct
     1441 ggaactaata taaaaatcat ctctgaaaga ttaggacata ctaatataaa aataactatg
     1501 aatagatatt ttcttgtttt aaacgatatg gataaaaaag cttctgaaaa tataatcaaa
     1561 gttctcttta aataacattc cattttatgt aagtaggcat tatatttttc ttaaatgtca
     1621 cttaaatgtc agttatatat aataatagta aacaactgta tttcactaat atttgctatt
     1681 atctaatagt atttatagtt tagatttgat ataatacatg tggtaaaatt ttgatttgta
     1741 aaacaaaatt ttacctacat atattaaaac aaatttaaaa ctaaggagac ctatatgtta
     1801 aaagtattaa aaggaaatat aatttttaca aaaacttcag aaactttcac agtatttgaa
     1861 aatagttata ttatactgga aaaaggtaaa gtaaagggaa tatttaaaga gatacctaag
     1921 gaatacaaaa acctaaaagt agtagattac actgataagc ttataatacc tggtatgaat
     1981 gacctccatg cccatgcctc tcaatttaaa aatcttggaa tggctatgga taaagagctt
     2041 ttgccatggc ttgaaactta tacatttcca gaagaatcaa attataaaga tatagaatat
     2101 gcaacaaaaa tgtataaaaa atttgtaaaa gaactttgga aatctggaac tacaagaata
     2161 gcagtgtttg caacagtaca taaagaagcc tctatgaaac ttatggatat attcaaagaa
     2221 gttggtcttg gagcatacgt tggtaaagta aatatgaata tgaattgccc tgatacttta
     2281 ttagaaaaca ctcaggattc tattactgat acagaagaaa ttataaataa gtatagtgat
     2341 acagattcta ttgtaaaacc aataataaca cctagattta taccaagctg tacaagtgaa
     2401 cttttaaaag gtttaggaga tttatgttta aaatataata ttcctattca atcacactta
     2461 tctgaaaatt atgatgagat tgaatgggtt agaagtctag aacctgaatc taaattctat
     2521 ggagatgcat ataatagata taatttattt ggtcaaactc ctactttaat ggctcattgt
     2581 gttcattgtt ctcaggatga aatagattta atgagagaaa ataatgttat ggcagttcac
     2641 tgtccagctt ctaactttaa tgttggtagt ggtgctatgc ctatcagaaa attaatcgat
     2701 aataatatta gattagcact tggatcagat ataagcggtg gacatacatt gtcaatattt
     2761 aaagcaatgg tatctgcaat acaattatct aaattatatt gggttaattc tggtaagaag
     2821 tataatttct tatctttatc tgaggctttt tatattgcaa ccaaaagtgg tggaagtttc
     2881 tttggaaaag ttggtagctt tgaagaaggt tatgattttg atgcattgat tattgatgat
     2941 tcaaatttaa atcatgacaa ttattctata cttgaaagac ttgaaagatt tatctatgtt
     3001 ggtgatgata gaaatatcat tcatagatat gtatgtggaa aactaatt
//
LOCUS       DAMJVI010000206         3046 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.212, whole genome shotgun sequence.
ACCESSION   DAMJVI010000206 DAMJVI010000000
VERSION     DAMJVI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.212"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            94..849
                     /locus_tag="PC545_003667"
     CDS             94..849
                     /locus_tag="PC545_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2458376.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDSTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKKDLTVEEILEKFEYAV"
     gene            946..1983
                     /locus_tag="PC545_003668"
     CDS             946..1983
                     /locus_tag="PC545_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2458377.1"
                     /translation="MIKSKKILSLIIAGVLGVSMLTGCSQNDGSNASNENKETDSKKQ
                     KNIGITQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFV
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     IKSDENAKSTK"
     gene            2793..>3046
                     /locus_tag="PC545_003669"
     CDS             2793..>3046
                     /locus_tag="PC545_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458378.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT         1168 a          393 c          461 g         1024 t
ORIGIN      
        1 ctttcctagc tactggtaac ttaaatattt cactaaaaaa aaacaaatgc aaagctagta
       61 ccaaaaataa atcagaaaaa agaggtgttg tagatgttac aaattaaaaa tctttcaaag
      121 agtttcccta atccatatgg tgaacctaat actatctttg aaaatttatc aatcgatata
      181 gaagatggtg aatttgtaag tatcataggt agtaatggaa ctggtaaatc tactttatta
      241 aatataatat ctgggcttat aaaagaatct tctggtcaaa ttactttaga tagtactact
      301 ataacaaatt tagctgagta taaaagaact caaatagtaa gtagagtttt tcaagaccca
      361 agccttggaa cttgtccttc tatgacggtt agagaaaatt tatctctagc acttaataaa
      421 gggaaattat tgaacttaaa aaaatgtctt cgtcataaaa cgaatgactt ggaacactta
      481 cttgaaggaa tttctttgga tttaaaaaaa tatttagata ttaaagtaca atatctttca
      541 gggggtcaaa gacaatcact atcactaatt atgtctagct tagcaagtcc taaggtctta
      601 ttactagacg aacatacagc agcacttgac ccaaaaacat caaatgaagt aatagagtta
      661 acagataaaa tcgtaagaga gaaaaatata actacactta tggttactca caacttgaaa
      721 cacgctcttc aatatggaga taggttgata atgcttcata agggtgaagt tgtattagat
      781 gtaaaagata aagaaaaaaa agatttaaca gttgaagaaa tcttagaaaa atttgaatat
      841 gcagtataag aaattaaaat cttagtttta atcagaaata tttataatct atcatatgaa
      901 tatatgaaca aatataatta ttaaatttta agggaggaaa ataaaatgat aaaaagtaaa
      961 aaaatattaa gtttaattat agcaggagta cttggtgtat caatgcttac tggatgttct
     1021 caaaatgatg gctccaatgc ttcaaatgaa aataaagaaa cagatagcaa aaaacaaaaa
     1081 aatataggta ttactcaatt agtggaacat ccatctctgg ataaagcaaa gaaaggattc
     1141 atcaaagcac ttgaagataa aggctataaa gatggagata atataaaaat agatttccaa
     1201 aatgcacaaa atgatatgcc tactacacaa agtattgcta gtaagtttgt atccgacaaa
     1261 aaagatttga tatatgctat atctactcca tctgcacaag cagcttataa tgctactaaa
     1321 gatattccaa taataatgac tgctgttaca gaccctgtag aagctggact tgttaaatct
     1381 cttgaaaaac caggtggaaa tgtttctggt acatctgatt atctttcaat tgataaaaca
     1441 ttagaattgg ttaaaacatt gactccaaaa gcaaagaaaa taggggttat atacaatact
     1501 agtgaagtta attcaaaaat ccaagttgat tctctacatg attatgctaa gaaaaataat
     1561 tatgaagtag ttgaaaaagg aatcagctct tcaagtgaag ttaaccaagc tatttctagt
     1621 ttagttggta aaatagatgt tttatatgtc cctactgaca atttaatagt ttcttctatg
     1681 ccaatagttt ctaaagttgc taatgaaaac aagattccta taatagcctc tgaagaaggt
     1741 tctgtatctt ctggtgcttt agcttgttgt ggaatagatt atgaaaaact aggttataag
     1801 gctggagaac ttgctattga agtattagaa ggaaaatctg ttggcgatat accagttact
     1861 acattagatg aaactgaaat aataattaac gaagatacac taaaagcact tgacatgcaa
     1921 aagttatcag cagataatat aaagtatata aagtcagatg aaaatgcaaa atctacaaaa
     1981 taaaacttat taatttcaaa aaataggtaa tatgaagtag gtaatatgaa aactttatat
     2041 aataagtact cttatccacc ctaacccttt gaaaattgtg cttattgtag caatttgaat
     2101 attgcatata aagttattca ttttccataa aagtcgtcta tattgacgac tgattatgaa
     2161 agttatataa ctttctaatc actacttgag ctgtccaaaa gaattttaat attctccaga
     2221 cagctttttt ttatgatatt tgattgatat acatgtctag ttattccata tttagtaaat
     2281 attttgataa aatttccaca ttcacttata acttaggata acgttttttt tcaaacaata
     2341 cattaattta aaagtattct gagtatacat tatttagtat tttaattaaa tttagcagtt
     2401 gacttttatt attgtataat ttataattta taacaagtcg agaatacata taattttctg
     2461 actaattaaa atattaaaag ggagagataa ttatgaatac tttattaaac aatagaaaat
     2521 attattatta ttatagccac tatttttagg gtttgtacct acgttttaac atagatacag
     2581 accccttaag actactaaaa ttttgggatt gtatctatgt tggctatatt gtttataaag
     2641 ttttcattta actatataag agccacatag attaaactat gtggcttatt cgtgttttta
     2701 ccacatagtt ttgtacacta tgtggttttt ttatacacaa aattagcaaa taattaaaat
     2761 tataaaaata ttttcaaagg agtgttttat ttatgtcagg tattatatct gtaatgacgc
     2821 aaagtttaat tttatcgatt atggcactgg gggtttatat aacttacaaa atcttagatt
     2881 ttccagatat gtctgctgat ggtagctaca caatgggtgc ttctattgta gcattttctc
     2941 ttactaatgg tatttctcct gtagtagcaa ctttaatggc tatcttatgt ggttgtactg
     3001 ctggtcttgt tacagggatt cttcacatta agtttaagat atctaa
//
LOCUS       DAMJVI010000207         2947 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.213, whole genome shotgun sequence.
ACCESSION   DAMJVI010000207 DAMJVI010000000
VERSION     DAMJVI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.213"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            125..1234
                     /locus_tag="PC545_003670"
     CDS             125..1234
                     /locus_tag="PC545_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDF2458379.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKISRRIIEL"
     gene            complement(1611..2795)
                     /locus_tag="PC545_003671"
     CDS             complement(1611..2795)
                     /locus_tag="PC545_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458380.1"
                     /translation="MQKSVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNNCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGVVLLVFTIFGAMLVSKAATAMTIA
                     ILVCTLTIFIVGIKAKVPEITEILSNRTSFTPGYIKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMIISFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     VN"
BASE COUNT         1130 a          443 c          405 g          969 t
ORIGIN      
        1 agtatcttaa tatattttta agctatataa ataataatta aaaaatttac taaaaatact
       61 tataaatatg atattattaa ttggttaata aaaaagaaaa tttttcatga aaggtaagat
      121 aacaatggat ttcaaaagct tagggattag tgaaaataca ataaatattc taaaaaaatc
      181 aggaataaca acacctacac ctatacaaaa agaaagtatt aaactaataa aagaaggaaa
      241 agatgtaata gcagaagctc aaactggaac aggaaaaaca ctagcttttt tacttccgat
      301 atttgaaaat atatcattag atataaatga tatacaagta cttatacttt ctcctacaag
      361 agaactagct attcaaataa cagaagaagc tatgaaactt aaagaatcta aagatgtaag
      421 tattcttgca gcatatggag gaaaagatat tggttctcaa ataaagaaac taaaaggaaa
      481 tattcatatg attatagcta caccaggtag gcttttagac catctaaata gaaaaacaat
      541 agaccttagt aagttaaaga cttttgtttt agatgaagca gaccaaatgt tgcttatggg
      601 atttaaaaat gaagtggaag ctattttaaa agagacttca aacaaaaagc aaacactatg
      661 cttttctgca actataaatt ctcaagtaaa aaaattagct tatagataca caaaaaatcc
      721 tgttgtagtt tctattcaaa aagaagaaat cactcttaat aatataaaac aagaagtagt
      781 agaaactaca gatagaaaaa aattagatgc cttatgtaag gtactagatg aagataatcc
      841 ttttatggca ataatatttt gtagaactaa gagaagggtt gacaatcttg aagaagctct
      901 tgctataaga ggatataatt gtcaaaaact tcatagtgat attgctcaat caaaacgaga
      961 aagaataatg aaatcattta gaaatttaga tatacaatac ttaatagcta cagatgtagc
     1021 ttctcgtggt cttgatatat ctggagtaag tcatatatat aattacgatt taccagaaac
     1081 accagaagac tacatccacc gtattggtag aactggtaga gctggtgaag aaggctatac
     1141 atgtgctttt attgacccta aaaatgaacg aatgttaagt gaaatagaga cggctataga
     1201 atctaaaatt agtagaagaa ttatagaatt ataatttata gattgcttaa aataccaatg
     1261 ccacacaaac actgtaatat atagaaagct acagaaaaca tgtatcaatc tatactttgc
     1321 tgttatacta ttttatatta aagaaaaaag aggctatcca aagtaggatt agtctctttt
     1381 attgtcaaaa taatgaaaat atatttgact tcatataaaa aagagtgcct tataaactaa
     1441 aagtccattt tgagacaccc tctttttatc ttttattctt agttttatat tgcttgttta
     1501 tagttgttta atattagatt ttgcaaataa tcttaataaa catgcagata tattatctat
     1561 agtattctta tctatagtat tctctttagc aatatttttt gctactacat ctaatttaca
     1621 ttttcattta attccgctgg ccatttagct tctggatttt cctttgcaaa cttcttattc
     1681 ttataatgtc ctagtgtaag gaatggaatt actatagtga atagaccaag ataaccacaa
     1741 tatccatatc catatttaac aatattactt aatcctgtaa gtgatataag cattgacact
     1801 accattattg cacaagctgt aaataccctt cttttcattc ttgaagaaag gaaagaaagt
     1861 gctttatggt cttcaaatct atttacaaga ccaaatatag tagttactcc tgtagacatt
     1921 aaacaaagga ataatgcaac attataaatt atgtatatag tatgatttcc agattgacct
     1981 gcaacataaa gagtagggag tgttgtttgc ccagcagcaa taatttctct ataccatccc
     2041 attagcatag ttacagcaag tcctaatgca attgcattca taacaaatga aattatcatt
     2101 gccttagtag cttcttttga gttcttaaga agtggtcttg aacatcctgc aagtgtaggt
     2161 ataacaaccg attgaaaacc tgcataaata aaagtattaa gtattggctt gatatagcca
     2221 ggtgtgaaac tcgttctatt tgataatatt tcagttattt ctggtacttt agcttttata
     2281 ccaacaataa aaatagtaag agtacatact agaatagcta ttgtcattgc tgtagctgct
     2341 ttagatacaa gcatagcacc aaatatagta aagacaagaa gtacaactcc aataataaat
     2401 acagctacaa aataatttac tcctatactt tgaaatactg ctgctgcacc tgctataaca
     2461 gcacttacag ctattaaaac cattagatag taatatatct cccatattat ttcaagtttt
     2521 ggatatggtc tccaaagctc acaaaataaa tccttataat tcctacaatt gttattatta
     2581 tacattatga taacttctct catggtagca gtaagtataa tcattgctaa aattgccata
     2641 agtggtgctg tccatccata ctgcacatag tactgagttt cttgattccc tgtagcaaat
     2701 cctcctcctg catgtgaact aaaaagaacg cttgcaacac caaaaatggc taacatagag
     2761 cccgttactt ttgctttctt tacacttttt tgcatgaaat catcctccct aaaatgatat
     2821 taaaagtttt tattttaaca ctttattgat aaaacaataa catttttgct cattaaattc
     2881 aactatattt ttccaatgac tttacatttt caacttattt ctacctttat attaaatatt
     2941 ttttata
//
LOCUS       DAMJVI010000208         2916 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.214, whole genome shotgun sequence.
ACCESSION   DAMJVI010000208 DAMJVI010000000
VERSION     DAMJVI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.214"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(449..1156)
                     /locus_tag="PC545_003672"
     CDS             complement(449..1156)
                     /locus_tag="PC545_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDF2458381.1"
                     /translation="MIFTKSNKYDKDFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLAKEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISIDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSITWWNKLWSESESVIVEKCEALNCHDEAWKDWINCDNSYAINDKKMME
                     VENGKYFNTISLIARTK"
     gene            complement(2031..2900)
                     /locus_tag="PC545_003673"
     CDS             complement(2031..2900)
                     /locus_tag="PC545_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDF2458382.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITPEFRKKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKNRRISFYVLHSPLDNYSDYSTSVSFAKAMGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMGMVDYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT         1013 a          467 c          256 g         1180 t
ORIGIN      
        1 ctattaaaat aatttttaat agtataataa atatagttct ttaatctttt gggtatatat
       61 acttaatcat tttgaaattt gatttaaagt ataataaaat atataaaatg gtactataaa
      121 atttttaatc ttcatcaata ccttttttgc tttcattgtt aaaactattt tttctattag
      181 tttatttctt ataaaacaaa catttagtat taaaatatat atcacacaat agtatttgca
      241 atttaacttt aaaaaataaa aatacccaac tgacctaagc aactcgattg ggtataaata
      301 atctaaaaaa tcatttttgt ataaactctt tatttaactt acatttttat ttaccttcta
      361 ctttaattca acttatattt ctatgcttct tatactctca ttcaacttat atttctactt
      421 atcttatact tttataaatt catcacaatt atttagttct cgctatcaaa gaaattgtat
      481 taaaatactt tccattctct acttccatca tttttttgtc atttatagca tatgaattat
      541 cacaatttat ccaatccttc caagcttcat catgacaatt taaagcttca catttctcta
      601 caatcacaga ctccgattca gaccaaagtt tattccacca tgtaattgaa tggaagttca
      661 tattatcttg ccaaaatgga atgagttcat ctggtataca atccacgaaa tcttctttta
      721 atccaggcat tgccatagca agtattcccc cctcttttac aagaggtgat atatgattgt
      781 ctaaaaatcc ttctttgttt ccaaaatagt gataagaatc gatacttatc accgcatcaa
      841 agaaaccttc tgcataaggt agttcatgtg cctctgcctg tattggaaat attttatcat
      901 ccaatttaaa ctctttaaat cttttatagt tttcagttgg ttctatccat aaatctgttg
      961 caaatacagt agcatcaaat tcctttgcta aaaatatcga agatatacct ttaccacaac
     1021 ctaagtctaa aatacgcatt cccttttcta gttttatctt acttgttaat tcttccaata
     1081 tttttataca attaggtccc atcatatttt tcatgagaaa gtccttatcg tatttattac
     1141 tttttgtaaa tatcattcta tctttccttt ccctatcagt tattatttat taaattttat
     1201 acgtttgatt ttttattttt tatatgcttc ttatattgag caatctcttt tttattcatt
     1261 ctttaacaga caattttaat gtgtaatttt atccactctg tgatagtaac tttaatgaat
     1321 aatcttatct actgtgatag ttattttagt gagtaatttt atctactcca cagcaatcgc
     1381 tttaatgagt aatcttatcc actctatgat aattatttta gtgagtaatt ttatctactc
     1441 tacagtaatc gctttaatga gtaatcttat ccactctatg atagttattt tagtgagtaa
     1501 ttttatctac tccacagtaa tcgctttaat gagtaatctt atccactcta taatagtcac
     1561 tttaatgagt aatcataatt tttatatcat tttagatact tactctaata agaagtcatt
     1621 ttctaatact taataagttt taattacttc cactttaacc tatatactaa ttaacttatt
     1681 taatttagtg catatttgac actttacctt ttattatacc atttattatc ataaatataa
     1741 taattctatt tttttaaaat aaatatttat aataatctaa aataaatatt tataaattct
     1801 aatcaatcct aaataataat ctaaaataca tcaaaaaata aactaaaaaa tatcgaaaaa
     1861 taatttaaaa gacatcaaaa ctaaatcttc taatcagcaa ttaaaagtct attttcttag
     1921 tttaaaaaat aaagatagat tttttcaaaa cactggataa ataggtaaaa aaataaacag
     1981 ttagtaaaat aaatttttac taactgttta tttttattgt ttaatatgtc ctataaatcc
     2041 tctaagtaat actttccttg caaaaattct gattctaaac ctagattttt aaaataatcg
     2101 accatgccca tacacgcaaa tttttctgta gaataatgag tagcacctat aacattaatt
     2161 aaattatctt ttgctatttg atggaactct aatactggtt caaaatgttt taaaggctta
     2221 gtaaacccag taatatatag atttatacca agttcagcaa cttctcttgc tacaaatgga
     2281 taacttcctc cccctgctgc aatagcaact aaaccatctt ttaatacaga atctccataa
     2341 tcatacaatt tcacatcatg accaacagat tttttaatta attccttaat ctcttctatt
     2401 gattttagtg atgttttaca tataactcct acttcaattt tatcgtcata tttacaaaat
     2461 ggtttgacta tttctaaacc catagctttt gcaaaactaa cagaagttga atagtcacta
     2521 tagttgtcta gtggagaatg taatacataa aaagaaattc tacgattttt catctcttcc
     2581 atataagata gtggtatgtc gtgaaatgga aaaccttcat ctgatgctat gtatcccatt
     2641 gcatgatgtg aaaaaattaa aatatctttt tcatctttat ctataacttg cttaattatt
     2701 tctttatcag gaaaagtagt tgtgtatact ttattaatat tttgagcatt atctaatact
     2761 aatcctatgt atttttttct aaattctgga gtaataaaat cattttcaaa attcataaaa
     2821 ctccaatcat cattaatatt tttaatatta aattctaaat ctaatttttt ataaagttca
     2881 attgccttaa taataaccac tcctttataa taatat
//
LOCUS       DAMJVI010000209         2896 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.215, whole genome shotgun sequence.
ACCESSION   DAMJVI010000209 DAMJVI010000000
VERSION     DAMJVI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.215"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            185..1108
                     /locus_tag="PC545_003674"
     CDS             185..1108
                     /locus_tag="PC545_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HDF2458383.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTNIKKMKDICFSIEGDNEQDDLVIQNFIDDLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAISPLVKKNIENILDNGFTK
                     ALTGPVSRGDITPIRKHLSVLDKKDEDIYKILSLNLLKIIALGNDNSLKGIKNITIEN
                     AIENLISKSEKYKEIYKLLGGKNDEKYHTDF"
     gene            1083..1910
                     /gene="panB"
                     /locus_tag="PC545_003675"
     CDS             1083..1910
                     /gene="panB"
                     /locus_tag="PC545_003675"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDF2458384.1"
                     /translation="MKNTIQTFKNAKYEGKKLSMLTAYDYSIAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDAVKNAGR
                     LIKEGGAHAVKLEGGSNVIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKVQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMKDSIKNYILEVNTGTFPQEKHSFSINESELEKLY"
     gene            1929..2777
                     /locus_tag="PC545_003676"
     CDS             1929..2777
                     /locus_tag="PC545_003676"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDF2458385.1"
                     /translation="MLVKEIKLLRNIIKDWRKHGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIDKDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPVHFGGVCLVVSKFLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNKSLSLAKEELVKGNLN
                     PENIKELITAKINSEHLAKIDYVEIVDSETLQPVKQIEHSILVAIAVFIGKTRLIDNF
                     TFELNI"
BASE COUNT         1170 a          338 c          465 g          923 t
ORIGIN      
        1 tatagaatta attgtcaatt ttttatgaaa atggtaagat atttatggat ggtatcttgt
       61 tataagaact atactttaaa tgtttttaat tattttgtct tatgtaactc atatcctcag
      121 gaatgagatt agaaattaag atgttgtatc acaaagtgga ggtgatacta ttaaaatagg
      181 ctttattggt tctggaaaag ttggaacatc tttggcaaat tattttttat caaaaggtta
      241 ttgtgtttca ggcttttatg gaaggaatca attatcttta ttagaatcaa caaatttaac
      301 aaaaacaaag atttacagca atttgaaaga tataatttat gaaaacgata tattatttat
      361 tacaacatca gatgattcta ttgaaattat agacaaaaaa ctttccaaat ttaatttaaa
      421 acataaatac atttgccaca caagtggctc tttaaaatct agtatattgt tctgttcaaa
      481 aaaagcaggt gcattaattt attctatcca tcctatattt gcattttcca ataaaaatac
      541 caacataaaa aaaatgaaag acatttgttt ttccatagaa ggtgataatg aacaagatga
      601 tttagttatt caaaatttca tagatgattt gggaaaccaa ttttttataa gagataagga
      661 tacttcatcc acttatcatt tagctaatgt tttagtttca aatttagtat tatctttact
      721 agatatagga gtaagttact ttattaaatt aggtttatct gaagaggaat ctttaaaagc
      781 tatcagtcct ttagtaaaaa aaaatataga aaatatactt gataatggct ttactaaagc
      841 tcttactggt cctgtttctc gtggagatat aacaccaata agaaaacatc tatctgtatt
      901 ggataaaaaa gatgaggata tttacaaaat actatcctta aacctactaa aaataattgc
      961 cttaggaaat gacaattcat taaaaggtat aaaaaatatt actatagaaa atgcaatcga
     1021 aaatttaata agtaaatctg aaaaatacaa agaaatttac aaacttttag gaggaaaaaa
     1081 cgatgaaaaa taccatacag acttttaaga atgctaaata tgaaggaaaa aaactatcta
     1141 tgcttactgc ttatgactac tcaattgcaa aaataatgga tgagtgtgat ataaatggta
     1201 ttttaatagg agattcatta ggaatggtta taaaaggtga agaaaacact ctatctgtta
     1261 ccatagatga aattatatat catacaaaag ctgtaaaaaa tggtgtaaaa aatgccttaa
     1321 tcgtaagtga tatgcctttt ctatcttacc atgtttctat agaagatgca gttaaaaacg
     1381 cgggtagact tataaaagaa ggaggagctc atgctgtaaa gttagaaggt ggctcaaacg
     1441 ttataaagca aatagaaagc attgtaaatg ctcaaatacc tgtaatgggg catttaggat
     1501 taactcctca atcagtaaat tcatttggtg ggtttaaggt tcaaggaaat accagtgaaa
     1561 cagcaagaca acttattgaa gatgctaaat taatagaaaa agcaggtgca ttctctattg
     1621 tccttgaagg agtgcctacg aaaatagctg agatggtaac taattctatt tcaattccta
     1681 caataggtat aggggcagga attaactgtg atggacaaat tttagtttat caagatatgt
     1741 tgggaatgtt tggagatttt gttcctaagt ttgtaaagca gtatgcaaat ataggagata
     1801 taatgaaaga ttcaattaaa aattacatcc tagaagttaa tacaggtaca tttccacaag
     1861 aaaaacacag cttttcaata aatgaatctg agttagaaaa attatactaa tattaaggat
     1921 ggtacaaaat gcttgtaaaa gaaattaaat tactaagaaa tataatcaaa gattggagaa
     1981 aacatggcta ttctataggg cttgttacaa caatgggatt tttacatgaa ggacaccaaa
     2041 gtcttataaa aaaagctgta aaagaaaatg ataaagtagt agtgagtgta tttgtaaatc
     2101 caactcaatt tggaccaaat gaagatttta atagctatcc aagagatata gataaagatt
     2161 ttaaatattg tatggatagt ggagcaacag tagtctttaa cccatcacca gaagaaatgt
     2221 atttaaaagg taattgtact accattaatg tttctggact tacagatttt ctttgtggag
     2281 ctaaaagacc tgttcatttt ggaggtgtct gcctagttgt ttcaaagttt ctaaatatag
     2341 taacacctga taaagcttat tttggagaga aggatgccca acaattagct gtaattaaaa
     2401 gaatggtcaa ggatttaaac atagatacag aaataatagg ctgtcctata attcgtgaaa
     2461 atgatggatt agcaaaaagt tcgagaaata catatctctc tgaggaagaa cgaaaatctg
     2521 ctctaatatt aaataagagc ttaagtttag caaaagaaga attagtaaaa ggcaatctaa
     2581 atccagagaa tataaaagaa ttaataacag ctaaaataaa ttctgaacac ttagcaaaaa
     2641 tagactatgt tgaaattgta gatagtgaga cattacaacc tgttaagcaa atagaacatt
     2701 ctatacttgt ggcaattgct gtgtttatag gaaaaaccag actaattgat aattttacat
     2761 ttgagttaaa tatatagtct ataattttat ataatatatc taaaaaataa tagaatacct
     2821 aaaaagtaat aaattaccta aaaaataata aattgcttag aaaataatag attacaataa
     2881 ctaaaatttt aaaatt
//
LOCUS       DAMJVI010000210         2864 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.216, whole genome shotgun sequence.
ACCESSION   DAMJVI010000210 DAMJVI010000000
VERSION     DAMJVI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.216"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            388..2199
                     /locus_tag="PC545_003677"
     CDS             388..2199
                     /locus_tag="PC545_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HDF2458386.1"
                     /translation="MKKKYDFLIYSAIWLVIIILFIFSLYHSIEHIKIISHTGTIRSE
                     TQKVVKQELNNERNDDLIKRLDNILIKLRTGNGENGFQRCDNKEFQQKLNQMDSMWES
                     MKKEIIKVRNGASGDKLYKLSEEYSVLSNQIVFISEKHSNAKLYSFATALFIYLIFST
                     ISLLIWEYYNKKRFKRIFYTDNLTKIKNQVAFENRAIEILHNASNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLIIVAAALSKTFNIKETCARIGSDNFVILAKYRDSLLEDIREM
                     LTNAIISELDMNVTQTISYCIGAYLVEIDNLGYKSINSIMDKANIAHKVSKTRGISST
                     VWYNENLLKQLQMENSIYNYMYKAIENEEFHMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDEFIPLFEKSGFIIELDFYMLKKACSFVRKTFMKKNQYTYPIAVNFSRVTIY
                     QNSFYQRFLDTVKEYEIPFKYIEIEVTESAFNEISQPVISILEELKKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKETFNREKVYSIIQCIIEMSHRIGMSVVCEGIET
                     KKDLEFLNTVKCDVGQGFYFSKPIEEKEFFNKYVTKK"
     gene            complement(2287..2864)
                     /locus_tag="PC545_003678"
                     /pseudo
     CDS             complement(2287..2864)
                     /locus_tag="PC545_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729118.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1084 a          294 c          406 g         1080 t
ORIGIN      
        1 taaattcaaa gagttaaatt gtaggtaaat agtattttaa ttatttatta aaatatagat
       61 tatgtatatg ccaaatttaa aaagagacat aaagacatta tacaaatata ttattggggt
      121 gatattttat aagaaaagaa gtgatgcagt aaattaattg agcaatttaa aaatgattta
      181 atgatagtgt tgatgaatgt ttaaaaagaa attttagaaa aacgtatatt gctgtttgtg
      241 ttggaggtga tgaatttgta taatttatag gatataaatg attaaactat ttaagataaa
      301 tacatatcac tgtaaaatat tgataatcat tttttataag agattgatta tttgcaaatt
      361 atatatatta caaatgaggt aacaataatg aagaaaaaat atgatttttt aatttattct
      421 gctatttggc tagttattat tattcttttt atattttctc tatatcacag tatagagcat
      481 attaaaatta taagtcacac tggtactatt cgcagtgaaa ctcaaaaggt tgttaagcaa
      541 gaattgaata atgaaagaaa tgatgacttg ataaaacgac tggataatat tctgataaag
      601 ctaagaacag gtaatggaga aaatggattt caaagatgtg ataataaaga atttcaacaa
      661 aaactaaatc aaatggattc tatgtgggaa tctatgaaga aagaaattat aaaagttaga
      721 aatggtgctt caggtgataa attatataaa ttgagtgaag agtattctgt gttgtctaat
      781 caaattgttt ttatatctga aaaacactct aatgcaaaat tgtattcttt tgctactgca
      841 ctatttattt atcttatatt ctcaacaata agtcttttaa tttgggaata ttataataaa
      901 aaaaggttta aaagaatatt ttatactgat aatttaacaa aaataaaaaa tcaggttgca
      961 tttgaaaaca gagcgattga aattttgcac aatgcatcta acaaagagta tgtactgcta
     1021 aatattgata ttgacaattt taaatatatt aatgatacac atggatatga atatggagat
     1081 aaagttctaa taatagttgc agctgcactt tctaaaactt ttaatatcaa agaaacttgt
     1141 gcaagaattg gttcagataa ttttgtaata cttgcaaaat atagagattc tcttttagag
     1201 gatattcggg aaatgctaac taatgctata atctctgaat tagatatgaa tgtaactcaa
     1261 actatatctt attgtatagg agcgtactta gttgaaattg ataatttagg ttataaatcc
     1321 atcaattcta taatggataa ggcaaatatt gcacacaagg taagtaaaac aagaggaatt
     1381 tcctccacag tatggtataa tgaaaatctg ttaaaacagt tacaaatgga aaatagtatt
     1441 tataattata tgtataaagc gatagaaaat gaagaatttc atatgtattt gcaacctaaa
     1501 tttcaaatat cttctttaaa tgttgtaagc gcagaagctc ttgtaagatg gttttctcca
     1561 gaacttggtt ttttatcacc agatgaattt attccattgt ttgaaaaaag tggatttatt
     1621 attgaattgg acttttatat gttaaaaaaa gcgtgttcat ttgtaagaaa aacgtttatg
     1681 aaaaaaaatc aatatacata tccgattgca gttaattttt caagggttac tatttaccaa
     1741 aatagttttt atcaaaggtt tttagatact gttaaagaat atgaaattcc ttttaagtat
     1801 atagagatag aggtaactga aagtgctttt aatgaaatat ctcaaccagt tatttcaatt
     1861 ttagaggaac ttaaaaagtt aggattttta atttcaatgg atgattttgg ttcaggatac
     1921 tcttcattaa gtcttttatg ttctctatct attaatggat tgaagttaga taaaagctta
     1981 cttaaagaaa cctttaaccg ggagaaagta tatagtatta ttcaatgtat tattgaaatg
     2041 tcacatcgta taggcatgtc tgttgtatgt gaaggtattg aaactaaaaa agacttggaa
     2101 tttttaaata cagttaaatg tgatgtagga caagggtttt atttttctaa accgatagaa
     2161 gaaaaagaat tttttaacaa atatgtaact aaaaaataat ataattattt ttatcagtct
     2221 atacattgag tcattttata tctcgttaaa tgactcaatg tactgttatg tattttaaaa
     2281 tataaattat ttaaaacgtt ttttatactt atatagaaat tcttctatat tttttgattg
     2341 ttctcttatt tcaactgctt ctatatctag taaataaagt atagaatcat ttgtaagttc
     2401 aacatattca ccatcttctg aaccgagtgc atgtctctca atttttaatc ctttttcttc
     2461 ataatatttt tttgtttcta tatataatga ttctcgcttt tgtatgtagt ctttcacttc
     2521 attatttgga aagttatatg aatccactaa tgtttcgcca tttataaaag ttttctcggt
     2581 agcaactttt tcatcccaac aatggatttc ttcctaagta aaaaatatct cagttccatc
     2641 tggaatgaat gaaaacctgg taataatata taaataatta ttgaaattta cttcataata
     2701 ttcaggaata tcttcaaatt ccatatagta atttgtataa ttaaaattac tattatcttt
     2761 ttttacaaag ggtatttttg caataaattt atatattgat tgttcatttt taaaaattcc
     2821 tatatcataa cttttttcaa attgattaat ttttaataaa taca
//
LOCUS       DAMJVI010000211         2859 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.217, whole genome shotgun sequence.
ACCESSION   DAMJVI010000211 DAMJVI010000000
VERSION     DAMJVI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.217"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            378..1421
                     /locus_tag="PC545_003679"
     CDS             378..1421
                     /locus_tag="PC545_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458387.1"
                     /translation="MLKKWFGIVKKRQKSESAKEEGEVILKNEKILSEEKLIDEEGVV
                     VNIDNEILTKVEVVNDDNEIEEKIIEEDWLISENTIKLDDKEAIINDKNIELCKKEVQ
                     VESEKIGLNKFEGLDQNENYNLEKNVIEEKKVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHIQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKS
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYRFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDILEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            1532..2674
                     /locus_tag="PC545_003680"
     CDS             1532..2674
                     /locus_tag="PC545_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2458388.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKVKKREIK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKSNYLQVRASHILIKTVDDKGKQVSSSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEVKSDLVSSLTSEKQNLLIQNLKEKYNVKITDKYQMKAKE"
BASE COUNT         1295 a          229 c          494 g          841 t
ORIGIN      
        1 attgttggat tatgtaaata attaatatat aataccatta tgggcaataa atacaaatca
       61 aatgataata tagtctattt atgtaaatgt tatattgtat actaagtata atagtaagtt
      121 ttttgatagt ggtgtagaaa gtaaaaatct actagcctaa attgatgtat ggattagttt
      181 aggaacagta attgcatatc ttaagtttga tgtcatgttg ggctactaga aataggtcat
      241 ctttttcact tgaatatctt ttctttaaac atggagagta tcatatcaaa aacatatgaa
      301 aattaagtga tttataagaa cagtaatatt ttatatgata gtatagaata gtaataaagt
      361 attaggagga acattgagtg ttaaaaaagt ggtttggtat tgtgaaaaaa agacaaaaaa
      421 gtgagtcagc aaaagaagaa ggtgaagtaa tattaaaaaa cgaaaaaata ttatctgaag
      481 aaaagttgat agatgaagaa ggagttgtag ttaatattga taatgaaata ttaactaaag
      541 tagaagtagt aaatgatgat aatgaaatag aagaaaaaat aatagaagaa gattggttaa
      601 ttagtgaaaa taccataaaa ttagatgata aagaagcaat tattaatgat aaaaacatag
      661 aattatgtaa aaaagaagtt caagttgaaa gtgaaaaaat aggtttaaat aagtttgaag
      721 gacttgacca aaatgaaaat tataatctag aaaaaaatgt tattgaagaa aaaaaagtaa
      781 gtgaatgttt gacagaagaa gatttagagt acataaaaga aattaaaata aaaagaggaa
      841 aaagtataaa agctataaac ttgtatacta aagaagagtg ggtttttgac actcatatac
      901 agtgtagtaa aaaactcaaa gttccattag ggtacataag agaaaattta aaatatggat
      961 atatggatta ctttggagat gcaataaatt atttaagtga agtattaaat atagatgaat
     1021 actgcaaaag tgaatggagc tatctcgata atagcaaatc tccatctgag atatttaata
     1081 ttctaaacaa taaaatattt agtataaggc tttctaatga aaaaagaaat gaaattttga
     1141 caaatgataa aattgaagca ttaaaaatga attatagatt tgaatgtatt gatgaagaat
     1201 atgatgaata ttttaaaaaa tataagtcta taatcaaaag aggtggaaag aaaaaagttg
     1261 aattagtaaa taaaaaaggt gacattttag aaatatttaa gtctttagaa gaatgtgcca
     1321 tttatttgca aaaggaaaag aatgaagtta tacaaatgtt aaaatatgga gatacaaaag
     1381 taggaagaaa ctttataagg tatagtttga gaagtattta atttcatttt aaattcataa
     1441 gagtttcatt tctgattcac acatttaata ttattttaat gtaaaataat aaaggtgaag
     1501 tattgcacaa ttaaaattaa ggagggtatt aatgtctgaa gaaaaaaatc caaaagaaaa
     1561 tacattagat gaatctaaaa attcagataa tgtaaattct aaaaaagatg atgtgtcaga
     1621 tgaaaaaaat actgataaca aagttaaaaa aagggaaata aagctaaaca acaaaacact
     1681 taaagctatt gtagcaattg ttgcagtagg agctatatgt ttaggtggag gctatgtttt
     1741 tggcaaagaa gctggaagaa aattgcctgc tacttcaaaa cactataata gcagtgaggt
     1801 agtagctact attggggata aaaaaataac aggtgaaatg ttaagaaaga gaatggagcc
     1861 attattttat ttaaatgcaa aaactactat gactgatgac gaaatacgtt catatgaatc
     1921 aaatatgatt gaaactatga ttacatctga tatattatcc tctgaagcag caaaagagaa
     1981 aataagtata actaatgacc aactagatgc tcagtataat aatttaatga gtagtataga
     2041 atcagcaatg ggaatgacta aagaagagtt tttaaaacag tttgatttga ctgaaaaata
     2101 tataaaagat gatatgaaaa aagagctagt agcatcaaaa tatttaaatg aaaaatcaaa
     2161 agttactgat aaagaagcag agaactatta caataaaaac aagagcaatt atttacaagt
     2221 tagagcttca catattttaa taaaaactgt tgatgataaa ggtaagcaag ttagtagtag
     2281 caaaaaagct gaacttaaga aagaagctga agaaatactt aaaaaagccc aagctgggga
     2341 agattttgct actctagcta aaaaatactc tgaagatagt agtgcagaat caggtggaga
     2401 tttaggtttc tttggtaaag gtcagatggt ggaatctttt gaaaaagcag cctttgcatt
     2461 gaagaaggga gaggtatcta acaaacttgt agaaagtgat tatggatatc atataattaa
     2521 gaaaactgat gaaaaatatc agccatttga agaggtaaaa tcagatttag taagcagttt
     2581 aacatcagaa aaacaaaacc tacttataca aaatctaaaa gaaaaatata atgttaaaat
     2641 tacagataaa tatcaaatga aagctaaaga atagataaag tcaatttatt aaaaaaaaga
     2701 aaaattaact gtatggataa aagctatttt tactcttaat atagagagta taaaatagct
     2761 tttattattt tgcaataaaa tctatttata tatatgaagt gacaaagaaa taacatgcaa
     2821 aaagatatta aaaagaaata tttttaattt gaatttttt
//
LOCUS       DAMJVI010000212         2761 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.218, whole genome shotgun sequence.
ACCESSION   DAMJVI010000212 DAMJVI010000000
VERSION     DAMJVI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.218"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            137..1336
                     /locus_tag="PC545_003681"
     CDS             137..1336
                     /locus_tag="PC545_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458389.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDSNELD
                     HININFGYALNGYINSYYTINGSNKTKKTELIKLQKDSKNTNNLIDVSSGSDLIAHVG
                     FEMNEESKDINFVIDEKKEDLEKGVFPSEDSDKEIEYEKLDMNNFTRTRYKVDKSTDD
                     YYAKILSSKRYKKYLYVSVLYATSDSSENNKEYSSTIDIIKINPENKKIKTIKSINLS
                     NYFSEKYQNIENIGVSYASKYEDKMYILVNVLNTNIGDNSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKAKLEANKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYVLDN
                     REKRGIRSIEIDSNKIVAYTSRLNSILSWDDKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFEIK"
     gene            1345..2157
                     /locus_tag="PC545_003682"
     CDS             1345..2157
                     /locus_tag="PC545_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458390.1"
                     /translation="MKDLIRLFEFELKRNIKNYIFIIICCCSFVILNIVKNLNDYNYI
                     IENAVKSEQLTKIGNTIETVNVTGFSSFRNIMGSTELWFMFGIIACICYAFFIWYRDF
                     NGRSKSIYTLIMLPKNRINIYISKLLNILFLVYSYTVVLTISLFIASKLLPRHMLGNV
                     TNYGFVQETIYELKMLLPYSFEILFVEYIFLLIGFVSVVFTSILINKSIYKVSTLISF
                     LFLVIEILVFIISIEFIIPYEYSDVFTVLYSSINIFVCSLISNKLLKQKIDF"
     gene            2168..>2761
                     /locus_tag="PC545_003683"
     CDS             2168..>2761
                     /locus_tag="PC545_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458391.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYEEVIISKNGVRNNTNPKSRIRNDYLNIDKKYSDFVNDKG
                     KEFIYKDDLNIGEAYTSVKSDLVQGMNALNDCIKVSNKNLKSGVEENFDINLNKSDFK
                     ENSWKTLFNKIYKGNLYMVISETNISGSKVNNICILKI"
BASE COUNT         1177 a          214 c          408 g          962 t
ORIGIN      
        1 aatattataa tcttatagga tttcaattta acaatttagt agcagggtta ttaagttttt
       61 taattataat gtttattcta aatggaatat cttatttgct aattaaaaag aaagtatctc
      121 tttaaggagg ggtggtatga aattaaaatc taaaatatct atacttatta tatttttgac
      181 tttagcaata tattttagta taggtttatt aaataatgaa gataatttaa agataaaaac
      241 tataaaaggt gactctaatg aattagacca tataaacata aattttggat atgctttgaa
      301 tggatatata aattcatatt atacaataaa tggaagcaac aaaactaaaa agactgaatt
      361 aataaaacta caaaaggata gtaagaacac aaataattta attgatgtat ctagtggttc
      421 agatttaatt gctcatgttg gatttgaaat gaatgaggag tcaaaagata taaactttgt
      481 gatagatgag aaaaaagaag atttagaaaa aggtgtattt ccatcagaag atagtgataa
      541 agaaattgaa tatgaaaaat tagatatgaa taattttaca aggacaagat ataaagtaga
      601 taaaagtaca gatgattatt atgcaaagat actatcatca aaaagatata aaaaatactt
      661 atatgtatct gtactttatg ctacaagtga ttcatcagaa aataacaaag aatattcaag
      721 tactatagat ataataaaaa ttaatccaga aaataaaaaa atcaagacta taaaaagtat
      781 aaatttaagt aattattttt ccgaaaaata ccaaaacata gagaacatag gtgtaagtta
      841 tgctagcaaa tatgaggata agatgtatat tttagtaaat gtactaaata caaacatagg
      901 tgataatagt aattctaaat ttcatttact tgagtataat cttgaagaaa ataaggaaag
      961 cataatggaa ttaaaaactg atagcaaaat ggctttagca aaagcaaagt tagaagctaa
     1021 taaattatat atggtaatgt cgaatgatta tgataatagt tcaattacag agttaaattt
     1081 acttacctat aatatattag ataaaagctt agatgaagat aaatatgttt tagataatag
     1141 agaaaaaaga ggaattagaa gtattgaaat tgattcaaat aaaatcgttg catatacatc
     1201 aaggctaaac tctatattat cttgggatga taaatttatg ctatatataa taaataaaaa
     1261 ttctaaggaa attgtatatg aaggagaatt acaatttaac agttcaaata gtatgttaga
     1321 atttgaaata aagtaggtga gttgatgaaa gatttaataa gattattcga atttgaatta
     1381 aaaagaaata taaagaatta tatatttata ataatttgtt gttgttcttt tgtaatttta
     1441 aacatagtaa aaaatttaaa tgactataat tacataatag aaaatgcagt taaaagtgaa
     1501 cagttaacaa aaatcggaaa tacgatagaa acggtaaatg ttacaggttt ctcaagcttt
     1561 agaaatataa tgggttctac agaattgtgg tttatgtttg gtataattgc atgtatttgc
     1621 tatgcttttt ttatttggta tagagacttt aatggtagaa gtaaatctat atatacactt
     1681 ataatgctac ctaaaaatag aatcaatatt tatatatcta aattattaaa tatattattt
     1741 ctagtgtata gctatacagt agtacttaca atatctttgt ttattgcaag taagttgtta
     1801 cctagacata tgcttggtaa tgtcacaaac tatggttttg tacaagaaac aatatatgag
     1861 ttaaaaatgt tacttccata tagctttgaa atactatttg tagagtatat atttttattg
     1921 ataggatttg tatctgtagt atttacatca attcttataa ataaatctat ttataaagta
     1981 agcactttaa taagttttct atttttagtt atagaaattt tagtgtttat aatttctata
     2041 gaatttatta taccttacga atacagtgat gtatttactg ttctatattc aagtataaac
     2101 atatttgtat gtagtcttat atctaataaa cttttgaagc aaaagattga cttttaggag
     2161 ggatattatg aaagcaaaaa atataggaaa atatacaata tgttttataa tagtatgttt
     2221 tttaatgatt actatgacct taatattgag ttattttaat aaggataaac tatctgtaaa
     2281 aactttaaaa ggcaatgtaa aagatatagg gagagtaagt gtagtatata cacctaatat
     2341 aagcaaattt tcttatgagg aggtaattat aagtaaaaat ggagtaagaa ataatacaaa
     2401 cccaaaatca agaattagaa atgattattt aaatattgac aaaaaataca gtgactttgt
     2461 taatgataaa ggaaaagaat ttatatataa agatgatttg aatataggag aagcgtatac
     2521 ctctgttaag tctgatttag tacaaggaat gaatgcttta aatgattgta taaaggtttc
     2581 aaataaaaat ttaaaaagtg gggtagaaga aaattttgat attaatttaa ataagtctga
     2641 ttttaaagaa aatagttgga aaaccttatt taacaaaata tataaaggta atttatatat
     2701 ggtaattagt gaaactaata tttcaggttc taaagtcaat aatatttgta tattaaagat
     2761 a
//
LOCUS       DAMJVI010000213         2730 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.219, whole genome shotgun sequence.
ACCESSION   DAMJVI010000213 DAMJVI010000000
VERSION     DAMJVI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.219"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(263..1075)
                     /locus_tag="PC545_003684"
     CDS             complement(263..1075)
                     /locus_tag="PC545_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDF2458392.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKKAVENPEEVENILMNKEKEMLDVIKSEEYKIIKLRN
                     YLMNLKGEVNMNDVIIKSLPKVKVASMRKVLKGYDELFNLFPNVMAEEMMKTGCTCAV
                     PDYCFNIYHDGEYRETDMDVEMCQAINDLKEDTDILKFKELDAVENAVCILHNGPYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRML"
     gene            complement(1151..2569)
                     /locus_tag="PC545_003685"
     CDS             complement(1151..2569)
                     /locus_tag="PC545_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDF2458393.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GMFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIAPMVIKQIAFGLAIGFILAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYLGGNGYLSVYISGIIIGNSRIPLKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNVFGIAMAISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVAIENDIFHIIFFIALLSVGIQGSLIPTVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDEGNKWANKSIIDSNIPDDILIVMIKRKEQVLIPKGS
                     TIIKTGDILVLSGDRIEELIQL"
BASE COUNT          961 a          487 c          276 g         1006 t
ORIGIN      
        1 taaaacatca atatttatat atattttgta tattaaatac aattatttat aaagtcttta
       61 ataaaaattt ataaatagag catctttaga aaactattat aataaaaaaa taagaggctg
      121 tctaaaaata tagataagcc ttttttcata atcgtaatta taaaaatata cttgatttca
      181 tatgaaaaag agtgtctcat aaactaaaat gtttactttg agacaccacc ttttttattc
      241 ttatttatac tttgtagaca tattataaca tgcgtttcac tggtatctga acttctgtca
      301 accagtcttc ttcactctct ttattccata taccatctat ataagattct ctaggatttc
      361 caaccacttt atatttattg tcctctatcc atttaaatgc atctgcgtaa gtttttccta
      421 atgttgaata tggaccattg tgtaatatac aaactgcatt ttcaactgca tctaattctt
      481 taaattttaa tatatccgta tcttccttta aatcatttat agcttgacac atttcaacat
      541 ccatatcagt ctctctatat tcaccatcat gatatatatt aaaacaataa tcaggaactg
      601 cacatgtaca ccctgttttc atcatttctt cagccattac atttggaaat agattaaaaa
      661 gttcatcata cccctttaac acttttctca tagaagcaac tttaactttt ggtaaagact
      721 taataattac atcattcata ttgacttctc ctttcaaatt cataaggtaa ttacgtagtt
      781 taattatttt atattcttca cttttaatta catccaacat ttctttttct ttattcatta
      841 aaatattttc aacttcttca ggattttcta cagctttttt tatctcagct aatgataaac
      901 ccatttgttt taaagttatt atctgatgta atttaggtaa ttgctctgtt gtataatacc
      961 tatattttgt aaattcatct acatattctg gttttaacaa ccctatatct tcataatacc
     1021 ttaatgtctt tgtagttatt ttatttattt tagaaaacat tcctatagaa aacatctcat
     1081 cacctgcgca ttagatttga ttaatttaag tttaaacttt ccccttaggt taaagtcaat
     1141 acaaaataaa ttataattgt attaattctt caattctatc tccactcaat accaaaatat
     1201 cccctgtttt tataatggtt gaacctttag gtattaaaac ctgttccttt ctttttatca
     1261 taactattaa tatatcgtct ggtatattag aatctattat actcttatta gcccatttat
     1321 taccctcatc aacatttact tcgataagtt tagagctaat ttctccatca taatcattaa
     1381 atgtttttaa tacaggtgta ttatcatcaa ctaaatccaa ctttctggct acagttggta
     1441 tcaatgaacc ttgtattcct actgaaagca acgctataaa aaatattata tggaatatgt
     1501 cattctctat ggctactcct tgagtaacag cataaattgc aaaaactact gaagccgcac
     1561 ctctaagacc tacccacgat atgaataatt tttctttatt tgagaattta aattttgcta
     1621 gaactataaa tgttgctaat ggtcttgcaa taactatcat gaatatagat atggccattg
     1681 ctataccaaa tacatttggt aacttagaag ggaaagataa caatcctaac ataaagaata
     1741 acattatttg cataatccat gaaattccat caaagaaatg aaccaaactt tttttaagag
     1801 gtatcctact atttcctata ataatacctg aaatgtaaac acttaaatat ccatttcctc
     1861 caagatactc gctaaatgca taagaaagta ttgcaatagc tgttataaat atagtataaa
     1921 atccttctat ctcgaagttt gaatgtctta aaatataaac agataatttt gctagtataa
     1981 atcctatagc taacccaaaa gctatttgtt ttattaccat tggtgcaata agttctaccc
     2041 cagaattatt catcaatgtc aaaagaatta gtgtcaacat atacgctatt gggtcattac
     2101 ttccactttc aacttctaat aatgatgcaa gtgaaccctt taagtttagt ttttgagaac
     2161 gcaacacagc aaacacagat gcagcatctg ttgaagctgc aattgcccca attaataaac
     2221 cttcaagtaa actagttcct attaccatgt ggcaaaacat acctgtaacc ccagccgtaa
     2281 ttattacacc cagtgaagac attaatatag aaggtatagc aacaggttta gccatattcc
     2341 agtttgttcc aaaccctcca aaaaacatta taaatattag tccaatagaa cataattgtt
     2401 ttgtcagttc atagttatca aaatagattc ctaccatccc atcagaccca aataacatcc
     2461 ctagtaagat aaaaatcaat aatattggta ctccaaattt atacaatacc ttgctagaag
     2521 ttatacaaat caatagtact aacccactta taatcattaa ttctgtcata gcatcaactc
     2581 catttcatat aaattttgta gattaattta catctacaat tttattactt attcaaatat
     2641 aacatgacat aaattttata agttatggta cataaagttc aacaaaaatt tcacaataca
     2701 ttaaaatgtg agtgttatac ttaaatttat
//
LOCUS       DAMJVI010000214         2663 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.220, whole genome shotgun sequence.
ACCESSION   DAMJVI010000214 DAMJVI010000000
VERSION     DAMJVI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.220"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            262..2280
                     /locus_tag="PC545_003686"
     CDS             262..2280
                     /locus_tag="PC545_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2458394.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKEKPDADSITLKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQELEKMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNPRDVIIIGDENAISKTTANQIKSTVNASQTRLKGSNRY
                     ETSLLIAKEIDKNHDVEKVYITNANGGEVDALTIAAKAGQDKQPIILTDKNSITDNTY
                     KWLKSEDLQNAYFIGGPQMISTNVINKVNDITKDNVTNNRVYGADRHETNANVIKKFY
                     TDDELEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVY
                     KIGGGLTSKVMNSIASSLSKHNTTPTEPGNSGGKTVMIDPGHGGSDTGTTGKPLGGIK
                     EKDYTLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNAS
                     DTTGNGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLY
                     VLRNNDMPAVLVECAFLDNEKDMSLLNTSNKVKEMGTQIGKGIEDSLK"
BASE COUNT         1100 a          306 c          431 g          826 t
ORIGIN      
        1 ttgtattaaa tttaataaac ctacaattgt tatatgtttt tatttgtctt ttatagattt
       61 ttttggcaat aaatgatata gtgaagataa ttaaatatat tcaatatatt taattttttt
      121 attttttgtt tttataattt ttttagaagt ttatttttta tgtacttatt tataattttg
      181 ggtaaaaaca ctttgatata atttcaaaaa ttaaaaggtt aaaaaacaaa gtagaaattt
      241 atgttaaata aggagattaa tatgagaaga aatacaaaat tattaacaac agggattctt
      301 tcaatggcaa tcgtcgcacc tacaatggca tttgctactg aatctaatgc tatggaaaat
      361 aacgctgatt taaatataaa cttagagaaa aaaagtatcg ttttaggtag tacctcaaaa
      421 gtcagtgtta agtttaaaga aaaaccagat gcagatagca ttacattaaa gtataaatgc
      481 tatgatatgc cattggatac aactttaaac tacaatcaat caactgagtc atatgaagga
      541 actatcaatt ataataaaga cccagaatat ctaaatgttt gggaactaca agggataaca
      601 ataaacagca aaaataatcc taaaacttta aacaaacaag aactagaaaa gatgggatta
      661 aatttaaaag actataatgt aacacaggaa tgtataattg aagatataac ttctagaaaa
      721 gatgtaaata aatatttaag aaaaacttct gcacctatta cagaacttac aggaagtgat
      781 agatacgaaa cagcagttaa aataagtaaa gaaggctgga aaaatggttc agataaggta
      841 gttataataa atggggatgt aagtatagat ggcattatat caactccact agcaactaca
      901 tataatgcgc caatactttt ggttgaaaaa aacaatgtac ctaatagtgt aaaatcagaa
      961 ttaaagcgtt taaatcctag ggatgtaatt ataattggag atgagaatgc tatttctaaa
     1021 actactgcta atcaaattaa atcgactgta aatgctagtc aaacacgtct aaagggctct
     1081 aatagatatg agacatcttt attgatagca aaggaaatag ataaaaatca tgatgtggaa
     1141 aaagtgtaca taacaaatgc taatggcgga gaagtggatg cacttactat agcagcaaaa
     1201 gcaggtcaag acaagcaacc aattatatta actgataaaa atagtattac agacaacaca
     1261 tataaatggt taaagagtga ggatttacaa aatgcttatt ttataggtgg ccctcaaatg
     1321 atatcaacaa atgttataaa taaggtaaat gatataacta aagataatgt tactaataat
     1381 agagtatatg gagcagatag acacgaaaca aatgcaaacg taataaaaaa attctataca
     1441 gatgatgagc tagaggctgt tttagtagct aaatcagatg tacttgttga tgctttagca
     1501 gcaggtccat tggctgccaa cttaaaatct ccaatactta taacaccaaa gacgtatgta
     1561 tctgcatacc ataaagaaaa cttagaagct aaatcggcta ataaggtata taaaatagga
     1621 ggaggattga cttctaaggt aatgaactct atagcatcat cattatctaa acacaatacg
     1681 actccaacag aaccaggaaa tagtgggggt aagacagtta tgattgaccc aggacatggt
     1741 ggttcggaca ctggaacaac aggtaaacca ttagggggta ttaaagaaaa agactataca
     1801 ttaaatactt cacttgcgac aactgaatat ttacgttcaa agggattcaa tgtaataatg
     1861 acaagagata cagataagac tttatctctt ggaaatagaa ctgctttatc taactctttg
     1921 agaccagatt tatttacgag tatacactat aatgcatctg atacaactgg gaatggtgta
     1981 gaagtatttt ataagcttaa agataaagat ggaggaacta ctaagactgt agctacaaat
     2041 atattaaata gaatcttaga gaaatttaat ctaaaaaata gaggtgcaaa aacaagaaca
     2101 ctatcaactg accctacaaa agattattta tatgttttaa gaaataatga tatgccagct
     2161 gtacttgtag aatgtgcatt tttggataat gagaaagata tgagtttact taatacatca
     2221 aataaagtaa aagaaatggg tacacaaata ggtaaaggca tagaagattc attaaaatag
     2281 tctacaatga gagcatagaa aaatttaaaa taaattaatc tataatttta aatttaaact
     2341 aaagctataa gataagaatg taattataat agtaataaag actgtatata cattgtgcat
     2401 ataaatgcga aatgaggtat atacagtctt tttataatta attgtaaaat gatttatgtt
     2461 atatgaaaaa tttacatata ttaaggctac agttaattgt caagagctgg agtattcatt
     2521 tgtaatacac aatataagct taattactat attttattgt gtgttttttg tggattttta
     2581 tgtcaataaa tgatatagta tagataatta aatgtattaa atatatttaa tttttttatt
     2641 ttttgagttt tataagttta ttt
//
LOCUS       DAMJVI010000215         2633 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.221, whole genome shotgun sequence.
ACCESSION   DAMJVI010000215 DAMJVI010000000
VERSION     DAMJVI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.221"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(225..1475)
                     /locus_tag="PC545_003687"
     CDS             complement(225..1475)
                     /locus_tag="PC545_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HDF2458395.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMDNNEPLYDFITDDKIEHTVWKIDNDDIIDNLVDKFEKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLGDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1508..2449)
                     /locus_tag="PC545_003688"
     CDS             complement(1508..2449)
                     /locus_tag="PC545_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HDF2458396.1"
                     /translation="MYNILVTDGIEKEAARKLRELDFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLENADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          796 a          499 c          244 g         1094 t
ORIGIN      
        1 atacacattt aaaaccttat aaatatacaa atacaattat atgaattttc atcctcaatt
       61 atagcataga atatgactat ctatatccta aaattatcca tttctaaaaa aaaggtaact
      121 attattaaga atatcaactt atagaattta tattccacat acgattttca atcaaaaaaa
      181 caagggctaa aaagtgaatt gtgcactttt tagcccttta ttaattattc atttatttca
      241 tgtaagaata gaccacatct tactttaggt tcaaaccatg ttgacttagc tggcattatc
      301 ttatttgcat ctgctactct tattaaatct tcaatactag taggatacat tgcaaatgct
      361 actttcatat cttcattaac tcttttttct aattcttcaa gacctctaat tccaccgata
      421 aaatcaattc ttttatctac tctaggatta tcaattccta aaatagcatc aataacatag
      481 tcttgtaata tagaaatgtc taatgagtca acagggtctt ctatttctaa taaattttta
      541 tttgctttca ttttatacca cttatcacct aggtacattc cgaaagttcc ttttttgtca
      601 ggtttatatt tttcttttcc ttctatttct tctagttcaa aattttcttt tattttattt
      661 ataaattctt cctcgcttaa accatttaag tctttgataa ccctattata atccaaaacc
      721 attaaattct catctggtgc aatcattgct atgaagtaat taaattcttc ttttccagta
      781 taatttggat ttttagctct catttctttg gctacatttt ctgctgaagc tgtcctatga
      841 tgaccatctg caatgtatag ataatctaat ttttcaaatt tatctactag gttatctatt
      901 atatcatcat tatcaatctt ccatactgta tgctctatct tgtcatctgt tatgaaatca
      961 tataaaggtt cattattatc cataaaatca ttgattatat catctatcac cctttgatgt
     1021 ttgtaagtta caagtatagt tcccgtatta gcatcacagt attttatgtg tttagttcta
     1081 tcaatttcct tttctggtcg agtgtactca tgttttttta ttgttccatt caagctttca
     1141 tcaactgata tacatgctac tataccaact tgagctctac catccatgat ttgtttatat
     1201 atatatatac actctttgtc atcttttact agaatttctt gttttcgaaa ctcatctaaa
     1261 ttttctcgcg cttttaaata aactttttca tcatataaat ctatattttc atctaaatca
     1321 atttctgatt tatctatatg caaaaatgaa tatggattcc cttctgcaat tcttctagct
     1381 tctttactat tcataacatc gtaaggaagt gctgctactt tatctactat atatttattt
     1441 ggtctaatcg ccttaaatgg tttaatcgtt gccattacta atttcctccc taattataca
     1501 aaatatttta taatgctacc cctattagat tgttgaagtc aagaaagaaa tcttgaatca
     1561 catttactat ttcgtctcct attctttctt gagcttctac tgtagatgcc ccaatatgag
     1621 gtgtaggaga aactcttgga tgatttacta agtctaaatt taatgtaggt tcttgttcat
     1681 aaacatctat agcagctgct gataatttac cttcatctaa tgcttttagt aaatcttttt
     1741 catcaactaa accgcctctt gcacagttta ttaaataagc ccctttcttc atcttattta
     1801 tttctttttc agttattact gcccccttat tcttatcaaa tggtatgtgt aaagataaga
     1861 aatctgcatt ttctaaaact tcttccatat cacagaattt ataattgtta aagccttgcg
     1921 cctcacccat tatgtcagta tatataacat tcatacctaa taattctgct ctctttgcaa
     1981 cttcttttgc aattcttcca aaacctataa gtccaagtgt ctttccatta atttctgtac
     2041 ctttatactt tttcttctcc cattttccat ctctcatagt tacatttgca gtatttatga
     2101 atcttgcaag aacaaataat tgacctattg taagctctgc aacagatata ctgcttgcat
     2161 ttggagtatt cataactttt atgccattag cttgagcata tttaacatct atattatcca
     2221 gacctactcc acctcttact atcaacttca actttttacc ctctaatgct ttatctatta
     2281 catctttagt aactttagta gctgaacgta ctacaagtac atcaacatct ttaagtttat
     2341 ctcctaaaac atctttttca taaaactgct ctatcacatt aaaatctaac tctctcaatt
     2401 ttctcgctgc ttctttctct attccatcag taactaatat attatacata acaaactcct
     2461 ccaattttta ttcacttaat ttttccaatt tttatttttt aaagtatttt catcaataaa
     2521 aaactttact taatttaact ttctgtttat ataattttca aaatattttt ttattaaagt
     2581 catatcaaat tttcctattg tttgttaaaa atttaatatg taatttttat aaa
//
LOCUS       DAMJVI010000216         2560 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.222, whole genome shotgun sequence.
ACCESSION   DAMJVI010000216 DAMJVI010000000
VERSION     DAMJVI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.222"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            636..2432
                     /locus_tag="PC545_003689"
     CDS             636..2432
                     /locus_tag="PC545_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDF2458397.1"
                     /translation="MKPTMEILTKLQENSKKNHDEVFTRLYRYLLRPDIYYVAYQHLY
                     SNKGAGTKGVTDDTADGFSEIYIENIIEALKNEMYQPKPVRRTYIKKSNGKMRPLGLP
                     VFTDKLIQEAIRMILEAIYEPIFSDYSHGFRPARSCHTALAQIKKEFTGARWFIEGDI
                     KGCFDNINHAVLVEIINQKIKDARFLKLIRSFLKAGYMEDWKYHKTYSGCPQGGIISP
                     ILANIYLNELDRHVMKIKKEFDVATKARYTPEYTKLVGLRQRLHNKIKNSNGIEREKL
                     IEEYKSATAQMLKLPAKQCDDKKIKYVRYADDFLIAVNGNRQDCEKIKQELTEFISTT
                     LKMELSQEKTLITHSNTPARFLGYDVRVRRDQQIKPKGKFKTRSMNNKVELSIPFKDK
                     IEKFLFSNGIVKQRSDNGKLEPIHRPQLLNRTDLEIVTIYNAELRGICNYYGLASNFN
                     KLIYFNYLMEYSCLKTLAGKHRSKVSKIRAMYKDGTGKWAIPYETKTVIKKMYFANYA
                     DCKGKKFTDIVPQTAKNYSHDVTTLESRLKAKICEVCGCTESDRYEIHHVNKVKNLKG
                     KSEWEKIMIAKRRKTIVVCHKCHMAIHHGEKK"
BASE COUNT          977 a          439 c          533 g          611 t
ORIGIN      
        1 ttagaagtag attaaagaaa gggggacaga aaaaaaaggc actaccccgt aagataacac
       61 gggaaaccac ctgcctaacc gaaaggtgaa aactgtacgg gaaaatagca cggcaggaaa
      121 gcagaaagtt gttcaaagac aatagagcac gactgaactg caaggttaag tgaatatgag
      181 gataaaacta gatttgttga atgtgagttc caaacctctg ttacgtgtgg acacaggaaa
      241 cttgtctgaa acctgtggta tgagtaacgc tgaaaataaa tgcctttcag tgggttatca
      301 attaactcat acggtacgct ggagaaccag tagtaacgga acgaaagcat atccgacaat
      361 tcacaccagc ctactacttt cgtcaactgg agattaccta aaccagaatg cctacaatgg
      421 ctatgtgtaa tgctcttgta gtgataaatc acaaggctat gctaagtgag atgacgctga
      481 aaatctggca aggtaacgga gctttcatag tagtctgaga accctgaaaa gacagggaaa
      541 gccgtaaaat cggatacggg aaaaccgttc acatggcgaa ggaaagcagt cttagcgaat
      601 taaaattaga aattgattag ggaggaaaac ctcaaatgaa accaacaatg gagattttaa
      661 ccaaattaca ggaaaattca aaaaagaacc atgatgaagt atttacaagg ctctatcgat
      721 atttactccg tccagatata tactatgttg catatcaaca cctctactcc aacaaaggag
      781 caggaacaaa gggtgtaaca gacgacacag cggacggttt tagcgaaata tatattgaaa
      841 atatcattga agccttaaaa aatgaaatgt atcaaccaaa accagtaaga cgtacataca
      901 tcaaaaaatc aaatggcaaa atgcgtccct taggactgcc tgtctttact gataagctga
      961 tacaggaagc aatacgcatg atattagaag caatctatga gcctattttt tcagactact
     1021 ctcacggttt ccgaccagca agaagttgtc atactgctct tgctcaaata aaaaaagaat
     1081 ttacaggagc acgctggttt atagagggcg acataaaagg gtgctttgat aacatcaatc
     1141 atgcggtact tgtggaaatt atcaatcaga aaattaaaga tgcacgtttt ctgaaactca
     1201 ttcgttcatt cttaaaggca ggttatatgg aagattggaa ataccacaaa acatatagcg
     1261 gttgccctca aggtggcatc atttcgccta ttcttgcgaa tatctacctt aatgaattag
     1321 accgtcacgt aatgaagata aagaaagagt ttgatgtggc aacaaaggca cgttatacac
     1381 cagaatatac aaaactggta ggactacgcc aaagattaca caacaaaatt aaaaatagca
     1441 acggtataga aagagaaaag ctgattgaag aatataaatc tgcaacggct caaatgctta
     1501 aactaccagc gaagcaatgt gacgacaaga aaataaaata tgtgcgttac gctgatgatt
     1561 tcctaatagc tgtgaatggc aatcgacagg attgtgagaa gattaagcaa gaacttacag
     1621 aatttataag taccacatta aaaatggaac ttagtcaaga aaaaactctg attacacaca
     1681 gtaatactcc tgctcgtttt ctcggatatg atgtcagagt acgccgtgac cagcagatta
     1741 aaccaaaagg aaaattcaaa acccgttcta tgaataacaa ggtggaattg agtattccat
     1801 tcaaagacaa gatagagaaa tttttatttt caaatggaat tgtaaagcag aggtcagaca
     1861 atgggaaatt agaacctata caccgaccac aactgcttaa taggacagat ttggaaatag
     1921 tgactattta taatgcagaa ttaagaggta tctgtaatta ttatgggttg gcaagcaatt
     1981 ttaacaagct aatctatttc aattatctta tggaatatag ctgtttgaaa acacttgcag
     2041 gaaagcaccg ttccaaagtt tctaaaatta gagctatgta taaagacggt acaggcaaat
     2101 gggctattcc ttatgaaact aaaacagtaa taaagaaaat gtattttgcg aattatgctg
     2161 actgcaaagg aaagaagttt actgatattg ttccacaaac agctaaaaat tattcccatg
     2221 acgtaacaac attagaaagc cgattaaagg cgaagatatg tgaagtatgt gggtgtacgg
     2281 aaagtgacag atacgaaatt catcatgtaa acaaggttaa aaaccttaaa ggaaaatcag
     2341 aatgggaaaa gataatgata gccaaaagac ggaaaacaat cgtggtatgc cacaagtgtc
     2401 acatggctat acatcatggc gaaaagaaat aacctgtaac ggagcaaaga tggagagccg
     2461 tgtgcattga gaaatgcaag cacggttcgg ggagaggact gcacaaacct accctagaaa
     2521 taaggaaagg cggtgctttc ctactctact atgtgggaaa
//
LOCUS       DAMJVI010000217         2496 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.223, whole genome shotgun sequence.
ACCESSION   DAMJVI010000217 DAMJVI010000000
VERSION     DAMJVI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.223"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            81..815
                     /locus_tag="PC545_003690"
     CDS             81..815
                     /locus_tag="PC545_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDF2458398.1"
                     /translation="MKQNKYDDDRFFNKYSKMERSTNGLAGAGEWHVLKKMLPDFKGK
                     RVLDLGCGFGWHCQYAVENGAISAVGVDISEKMLKEARNKTQLDNINYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGNFVFSVEHPIFTAYGSQEWYCDK
                     DGNNLHWPVDRYFEEGVRRSNFLGEEVIKYHKTLTTYLNALMKTGFEILEIIEPQPEE
                     KLLDTIPDMKDELRRPMMLLVSAKKR"
     gene            complement(958..1449)
                     /locus_tag="PC545_003691"
     CDS             complement(958..1449)
                     /locus_tag="PC545_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDF2458399.1"
                     /translation="MNNQQQEVYKKLDELSIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            1798..2181
                     /locus_tag="PC545_003692"
     CDS             1798..2181
                     /locus_tag="PC545_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458400.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKGYLYV
                     SSMYAIKEAKLESRVNSGRVKQYDG"
BASE COUNT          973 a          276 c          368 g          879 t
ORIGIN      
        1 tatataaaag tgataaaaac ttatattttg tatttaatag agtgttgcta agtgaatata
       61 tttaaaatgg aggaaacttt atgaaacaaa ataaatatga tgatgataga ttttttaata
      121 aatacagtaa aatggaacgt tctacaaatg gattagcagg tgctggagag tggcatgttt
      181 taaaaaaaat gttacctgat ttcaaaggaa aaagagtttt agatttagga tgtggatttg
      241 gatggcattg tcaatatgca gttgagaatg gagcaatttc tgctgttgga gttgatattt
      301 cagaaaaaat gttaaaagaa gctagaaaca aaacgcaact tgataatata aattacatat
      361 gtatgcctat tgaagatatt aattttccaa aagatagttt tgatgtagtt attagttcct
      421 tggcatttca ttatattcaa tcatttgaag atatatgtaa gaatataagt aattgtcttt
      481 caaataaagg taattttgta ttttctgttg aacatcctat atttactgca tatggaagtc
      541 aagagtggta ctgtgataaa gatggaaata atctacactg gccagtagat agatattttg
      601 aagaaggagt acgtaggtca aattttctag gagaagaggt tataaaatat cataaaacat
      661 taacaactta tttaaatgcg ttaatgaaaa caggatttga aatattagaa attattgaac
      721 ctcaaccaga agaaaaactg ttagatacaa ttcctgatat gaaagatgag ctacgtagac
      781 ctatgatgtt acttgtatct gcaaaaaaaa gataagtaaa tataaataat cattattgta
      841 gaaaaaaagt tgtagaaaat aattacacta atggagatga ttataccagt ggtattaatt
      901 acaccagtag taaggaggca aaagcattcg ttgcttttgc cattttttaa gttgcaatta
      961 tacttctacg tagtatattt cgtttccatt ttcttctata agctttttca tatcttcaaa
     1021 ttttaaaaat acagtagcag tattatcatt tggatgtata cctattttat ccatttttaa
     1081 taaatcctta tcaaatacaa cttttacaac agcctcttta tcatttaata taccaaaagg
     1141 agtcacagct cctttagtaa gtttcaagta tttttgaagt ctttcttctg atgcaaaact
     1201 aagaggttta ctatcaattt gttgacgaat atcttttaaa tctgcttttt tgtctttacc
     1261 taatacaact aaaaaatgtt cttttccttt agcatcacga agaaataggt tttttacaac
     1321 aaattccata tgtaatgttt ttaattcttc catttcttca attgtataaa ctggtggatg
     1381 atttacagcc tcataaggta tgcttaactc atctaatttt ttatatactt cctgttgttg
     1441 attgttcatg atttcctccc attagaaatt tattttatat ataaataata ttatcacaga
     1501 aaactttaaa agaaaacgtt ttatataata tagacaatag attatatttt tataaagaag
     1561 caagaatact caataaaact taaagaaatt aaacgatttc taaaaatacc ttgaaaatag
     1621 aaccttattt gccatttata aattatataa aaagggatta tataaagtca tatatgaaaa
     1681 aactatatta tactatcttt tctttttgga ataaactata ctttaaatat agatatttaa
     1741 tttaaataaa ttttacagta ttaatagtaa attatcttag aaaggaatgt gctttatatg
     1801 tctacagaca aagttgttgg aatcatagat gaagaaacgg cagaacttgc aggaattgaa
     1861 tatactggta aaatttatgc ttcttctggt gttataaaac atataaagaa aaaacataga
     1921 tgtcaactga gtaaggatat ttttaatgat ataatagaca ctataaaaat ggtattaaag
     1981 tcacctgaat atataggaag tcatcctaaa aaacctggaa aaagtgtgga gtttataaaa
     2041 aaaattgatg attatatttt agtagctaca gaattagata caaaaaaagg atatttatat
     2101 gtatcttcaa tgtatgctat aaaagaagca aaattagaaa gtagagtaaa cagtggaaga
     2161 gtaaaacaat atgatggtta acataatatt attaattttc cctaaaatat tgaaaaatat
     2221 tattatataa gttataatat taatatacac gtagatattt attagttaga tttgaggttg
     2281 gaaaaggcac ccaatacgct actatagagt agttaataga gatgcaggat acgccgccct
     2341 gccaaatcta attaaattaa aaagagagga ttaattcctc tcttattgtt tttttgagat
     2401 actaatttaa tctataatag aaagattgaa ggtcgtttat acaaaaaaat tttcagttat
     2461 atacattaaa ctaattaaat aagatattta taatct
//
LOCUS       DAMJVI010000218         2458 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.224, whole genome shotgun sequence.
ACCESSION   DAMJVI010000218 DAMJVI010000000
VERSION     DAMJVI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.224"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            205..345
                     /locus_tag="PC545_003693"
     CDS             205..345
                     /locus_tag="PC545_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458401.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(342..713)
                     /locus_tag="PC545_003694"
     CDS             complement(342..713)
                     /locus_tag="PC545_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458402.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTKISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1262..1519
                     /locus_tag="PC545_003695"
     CDS             1262..1519
                     /locus_tag="PC545_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458403.1"
                     /translation="MIIIRSQDKTNLMQINQIKIDGSQVYAVFESKIDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITSNKVRYNVNKIFQMPAK"
     gene            complement(1629..2309)
                     /locus_tag="PC545_003696"
     CDS             complement(1629..2309)
                     /locus_tag="PC545_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDF2458404.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVMIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSGLFRIDEINLIPFDSVGILTYILNIIMFMPLGFLL
                     PLIWKNFRNIINVSLTGLGFSLAIELCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISEKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          994 a          267 c          276 g          921 t
ORIGIN      
        1 gatttttttg ttattattac gagtttttta ttgcaactgt agaataaaag tattattata
       61 taattaagat aattatctag ggaaattttg gataatcaaa atatatttta taaaatcaaa
      121 atacaattac ataaacttta ttaaaaattt gttgggaatt tctgactaac attaagaacg
      181 taagtttgtt gggggatggt atatgtggaa aagtcaaaca ctactgttac aaaaaaagaa
      241 aaaattgaag atataggaat gaaattagaa catttattga gtgaagatat tactttttat
      301 gaattattac ttgagatttt gtataaagaa aaagaagaga attaattctc ttctttaagg
      361 aatcttttta aataggcatt atcaatcatt ttttctatag cttctctatc ttctttttca
      421 agagaatata ttttttctaa gagctttttt gttttttcac ttactgataa gttctctatg
      481 attaatttat cttctttgct tatttttgta ttatcattta cgcctaataa ataatctgtt
      541 gatgtttcta atgcttttgc taatctaact attatttcac ttttaggttc tcttaaatta
      601 ttttcataac gtgacaagct agcctcagtt ataccgactt tagtagcaag ttctttttga
      661 tttatatcta attcttttcg tagatttgct attctatctc ctaaactttc catatgtaca
      721 tcactcctta ccgaaattat aagttatttt gataaaaagt aaaataaaat taccaaaaat
      781 ataaaaaaaa aaggttaaaa taataaaata tattgaaaat taccatttgt atatgtataa
      841 tatgaattat acaaatggta atttgatgag aggtggtatg ttgaaataag aaactgaatt
      901 aattaataaa tacatagaaa gttttgaaaa cctttaattg aaatataaac aatatgtaca
      961 aaattttttg caagtaaaat taccaaatgt ataaaataaa tcagtagcat agataatttt
     1021 atgctttaat acattagatt atttaattta gttataatgg tcaaaaatgc aatctacaaa
     1081 taaaacaaag tattatcaaa aaagtattct ttaagaaaat tttttattag agatgtaatt
     1141 gatataaata atcgtattaa aaaataaaat actaagttta aaaatttaaa taatctataa
     1201 atcaattata aaatattatt tcttagaaat taaaatgaat taagtaaatg gaggaattga
     1261 tatgataata attagaagtc aagataaaac taatttaatg cagataaacc aaattaaaat
     1321 agatggtagt caagtttatg cagtatttga aagtaaaatt gatactgtaa aaataggaga
     1381 ttatgaaaat aatacaagag ctatacaagt gttggacaat atacaaaact tcattgaaaa
     1441 tggaacaaaa tatgattata taacatcaaa taaagtcaga tacaatgtta ataaaatatt
     1501 tcagatgcca gctaaatagt gagtgtgcat tttaatttaa taactaaagc ccactttctt
     1561 ttgtggtatg aaagagggct ttacatattt gtaaatatcg aagtacactt gttttatcaa
     1621 taaattattt aataaggtat aatggaccaa ttaaacaata aaaattctcc taaaacagca
     1681 agtattaaat atataatagc ttcatttttg taaagagagg taattttttc gcttatgtca
     1741 tcatcaattg taatagcaat ttcatcttga ataatatttt tatatataga tgggaaaata
     1801 tcttttttct ttataagatt aaataaatta tttacaccta cccatataaa atatcctaaa
     1861 acagcaccta atgtgttcat cattaaatca tcaatatctg tagctcttaa gttaaacaat
     1921 tgacaaagtt caattgctaa tgaaaaacct aatccagtta atgaaacatt gataatattt
     1981 ctaaaatttt tccaaattaa aggtagtaaa aatcctaaag gcataaacat aattatattt
     2041 aaaatatatg ttaagatgcc aactgagtca aaaggtatta gatttatttc atcaattcta
     2101 aaaagcccac tataccttcc tatatcccat atgctaccaa ttccagcttt actaagtgct
     2161 agatagatat ataataaaaa aatatatacc catactaaat gtatcattac attcttttca
     2221 actttcaaat tttttcgttt tataacgact atttggtata ttaagcatgg taatataacg
     2281 caaataaatg aataaaatgt ccatatcatt ttattttgtc ccctttcaaa tttatgtttt
     2341 tagttttata agtactatct ttatattaaa ctgtaagttt taaaaatctt ttaataaaat
     2401 cttaaaaatt tattaattta taaaaatata ttattaagat taaaaaatat atataatc
//
LOCUS       DAMJVI010000219         2410 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.225, whole genome shotgun sequence.
ACCESSION   DAMJVI010000219 DAMJVI010000000
VERSION     DAMJVI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.225"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            950..2305
                     /locus_tag="PC545_003697"
     CDS             950..2305
                     /locus_tag="PC545_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDF2458405.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYASKYLIYIFLTTAI
                     VHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDSQEELDTAFSVQYSFVNIGSFI
                     GTIAVGILYLKTFAKNGVLGFSQCFFIAAVLCVIGAIWFIYGWRFLGNAGKRPFKEGV
                     VAEKIEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVNMNVG
                     GFDVPLAWFDSLNSLVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFSRGVGAPETAKASILWIIMFGILLSLGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGKGYGYIYAFTLKFDMIKVYTIIPVILFVAAILLFLSDKTLSRLVEDD
                     K"
BASE COUNT          858 a          259 c          403 g          890 t
ORIGIN      
        1 gattattata tactgcatac aaaaatatat ttgagcaaat aagtaaagta ataaaaaaat
       61 caagatttca tatctaaaaa caattctgaa cattaattta atttatattt tcataggaaa
      121 tattttatat aatatacttg ataaatataa tttgaatttt tctaattatt tactgtcgat
      181 taatagattt ttttcttgca tataagtgca tatttatggt acgatttaca tggtgaaata
      241 aaaaagggga ggtgaaaaat aaatattcgt gaaaaagttt tcagtttgag gtattttatg
      301 aataatatat tttgaggtat tttataaata atatattata cataaagagt ataacctcaa
      361 taaatagtat caaagcgaat atatgggtaa ttactttaaa ataacagttt ttatgtttag
      421 attcacagtg tttattataa actaatgtgt aagaaataag cctaatctgt gaatagatag
      481 tagtttagtt atttaagttg agaattggtt gggtgaagta atatataaat ttaaaaaata
      541 aataaaaaca ttatttgaac aaaatatatg ttgctttatt cgagactcct ttaaaaaagc
      601 actaaaatgt aattacataa gttgctacaa aagggggatt ttagtttgta aaatactatc
      661 acgtaaaaaa taagaattgc atatggcgaa ggttttttca tggtattaga tgagaatata
      721 ttaatttata actatatctg aaaatagtga atttatataa tttaaattta tgattaagaa
      781 gtgtattaat tttattccat gagaatatgt actctacaaa taaatgcgca attatcttaa
      841 taataattac aaattgtttt acaaaataat aatattatat ctttcataaa agaaagaatt
      901 tggacaaaac gtaaaaaata aaaatacaat taagaaagga tttaaaatta tgagctcaac
      961 agtaaaaaag aagggtaaat ttcctatggg attttatatc tgctcaacaa ctttctcatt
     1021 tgaaagagca gcatattatg cttcaaagta cttaatatat atattcctta caacagctat
     1081 agttcatggt ggtcttggaa tagataaagg acaagctgct ataatgcaag ccaaccttgt
     1141 tgcattcaca taccttgcac ctataattgg gggatatata tcagatagat ggataggagc
     1201 tagatatact atacctatag gtatgcttat catgggagta ggatactatc ttggttctat
     1261 tgcaactaca gtttctatgg taaatgcaat gataatctta gtttcaattg gtactgcatt
     1321 ctttaaaggt aacgtatctg cggtaaatgg acagcttttt gatagtcaag aagaacttga
     1381 tacagcattt tcagtacagt attcttttgt taatataggt tcttttattg gtactatagc
     1441 agttggtatt ctttacctta aaacatttgc aaaaaatggt gtacttggat tttctcaatg
     1501 tttctttata gcagctgtac tttgtgtaat aggtgcaata tggttcatat atggatggag
     1561 attccttggg aatgcaggaa agagaccttt taaagaaggt gtagttgctg aaaaaattga
     1621 agaaaaagat aagtctcctc ttacaagcat ggataagaag agaatttggg ctattatact
     1681 tatatcattc ttctctgtaa ttttctgggt attctggtat ctaacttatc ttgcagttta
     1741 tgattatggt gcagcatttg taaacatgaa tgttggagga tttgatgttc ctcttgcttg
     1801 gtttgactca ttaaattcac ttgtatgtat agtattagga ccagttcttg gagcattatg
     1861 gtttaaactt gctagtagac cacaaggaga tatgagttta tttaaaaaga caggtcttgg
     1921 attaatattc ttaggtcttg cattcttaat gcttgttggt gcagaatttt caagaggagt
     1981 tggagctcct gaaactgcta aagctagtat actatggata atcatgtttg gtattctttt
     2041 aagtcttggt gaaatgttat tctcaccact tggtaactct tttgtaagta agtatgcacc
     2101 taagaagctt ctaggtgttc ttatgggagt atggacattt gctactttta tagctggtaa
     2161 aggatatgga tatatatatg catttactct taaatttgat atgattaaag tatatactat
     2221 aattccagta atactatttg ttgcagcgat attattattc ttatctgata agacattaag
     2281 tagacttgtt gaggatgata aataattagt aagcaattat tatgttttat aggttaatct
     2341 actgtaatta tgttgtatta gattgattta taactaatta ataataaaaa taaaatttta
     2401 cttctaaata
//
LOCUS       DAMJVI010000220         2319 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.226, whole genome shotgun sequence.
ACCESSION   DAMJVI010000220 DAMJVI010000000
VERSION     DAMJVI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.226"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(150..1442)
                     /gene="brnQ"
                     /locus_tag="PC545_003698"
     CDS             complement(150..1442)
                     /gene="brnQ"
                     /locus_tag="PC545_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDF2458406.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLI
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFSRAGKGLSIALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIISPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     EKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     IPLVNSLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          768 a          374 c          254 g          923 t
ORIGIN      
        1 atactactcc aaaaataaat ttttaaattt atcaaatagg attaataatg ttcttttatc
       61 tacaataatt taataattct tttaaatata aaaaggaatt gcctatataa tttaaaggca
      121 attccataat tatcaattag tttttattat tatgcagttt tactgctttt agatggaata
      181 aagtttccta ttattcctgc tattacaact ggaactatcc agttaaatcc taatgaagca
      241 aatggtaagc tattaactag aggaattttt acaccaaaac ttgttgcaac tgttaataca
      301 cttactaata atgccatgta tgcagcacat ttgaacacgt tgtcattttt aattttatta
      361 ctaaataatg ctagtataac tagagttata gttgctggat ataccatact tagtatagga
      421 gaagagaatt gtattattgt acttactcca aagtttgata tgattgcact aaatacacaa
      481 actactataa ctatatgctc atactttaat tttccatctg taagtgttga gaaatattga
      541 ccagctgctg aagaaagacc tatagctgtt gttaaacatg ctaatgcaac tataatagcc
      601 aataaaattt tacctgtagt tccaagtaaa gctgctgtta ttgcaactat taaagctgtt
      661 tgttgaacat cttgtccata ttgttgagac acagttgctc caagataaca taatccacca
      721 taaactaatg ccattcctat tactgcaatt acacctgcac ttattgtggc tttcatcttt
      781 tctttctcac ctgtataacc tttagctatt actgacatga ttataattga tgcaaataca
      841 ctagctgcca atgcatccat tgtttgataa ccttgagtta taccttctgc aaatacattt
      901 tgtattaaag aactgtctct tatttctcct aatggagata tgataccttt aactattaat
      961 actgctaaag ctataagtaa agctggagtt aatattgacc caactatatc tacaactttt
     1021 gatggtctta ttgttaaaac caatactaga gcaaagaata ctatagagaa tattactggg
     1081 cttattccag aacctattgt aggtaatata cccatttcat aagttgttgc tgctgttcta
     1141 ggtattgcta aaagtggccc tatacagatt accattgcac atcctaatgc aatacttaat
     1201 cctttgcctg ctcttgagaa catcttcgat gtgtctccat tgaacttagt tgctgctata
     1261 actgctaata atgccaaacc accatctgca aaaaggaatc ctatgaaagc tattagccaa
     1321 ctacttccag ataaaactcc tagatatgga gggaagatta agttccctgc accaaaaaac
     1381 attgcaaata gtgcaaatcc aattacaatt acatctttaa aattgatttg tttctcaccc
     1441 atttaaagca cccccatttg aattttacta ccttttacta aaaaataagt ccaatctctt
     1501 cattaaatga aaaccttttg acgagatttt atttaattat acatttttga cttaaatttg
     1561 tcaattatgt ttcataattt ataaaatctt cacaatattt aatacaactt tcttaattaa
     1621 acaaaatcaa cctgtatact ttaaacacag ttggtttttt gttttttctc taatttttct
     1681 tgataaaatt aattattctg aataattaaa atattaagat tttttagatt attcgatttg
     1741 ttattaagtg tattgcttta tatactgtat atatcactct ccttttttac attcatataa
     1801 tctttttaat tcaatttaaa ttttaaatat tttgtgttta ttatataatc tgaaaaaaaa
     1861 gcagtcaata gcatttttaa tttttttaat tttcaaaatt atgattctta aaaaatcgtt
     1921 ttccttttgt catagcacct atttacgtta attcttgtag tattttgtat ttattttgct
     1981 aaattgacag ttaactttat tcgtttttta ttctatttat gtagatttgc ctcttgtttt
     2041 ttagtcttaa aatcattttt tctgaaaatt ccttattatg atttatcaac ttatttttct
     2101 tcatatcaat tactttctaa cttccaactt acttaaactt tgcagttgct agtattcacg
     2161 tttacatact tatctgtcat aattaactaa aaattcttaa tatatctctc aatgaaaaca
     2221 atttacccat ttacttaata catttatata atcatatgtc ttaaattttg ttttattatt
     2281 ttattattgt cacaatttta tttatattat atttttgta
//
LOCUS       DAMJVI010000221         2162 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.227, whole genome shotgun sequence.
ACCESSION   DAMJVI010000221 DAMJVI010000000
VERSION     DAMJVI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.227"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..128
                     /locus_tag="PC545_003699"
     rRNA            <1..128
                     /locus_tag="PC545_003699"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            260..376
                     /gene="rrf"
                     /locus_tag="PC545_003700"
     rRNA            260..376
                     /gene="rrf"
                     /locus_tag="PC545_003700"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            383..457
                     /locus_tag="PC545_003701"
     tRNA            383..457
                     /locus_tag="PC545_003701"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Asn,seq:gtt)
     gene            462..536
                     /locus_tag="PC545_003702"
     tRNA            462..536
                     /locus_tag="PC545_003702"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:495..497,aa:Glu,seq:ttc)
     gene            542..617
                     /locus_tag="PC545_003703"
     tRNA            542..617
                     /locus_tag="PC545_003703"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Val,seq:tac)
     gene            623..699
                     /locus_tag="PC545_003704"
     tRNA            623..699
                     /locus_tag="PC545_003704"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:657..659,aa:Asp,seq:gtc)
     gene            710..784
                     /locus_tag="PC545_003705"
     tRNA            710..784
                     /locus_tag="PC545_003705"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:742..744,aa:Thr,seq:tgt)
     gene            799..883
                     /locus_tag="PC545_003706"
     tRNA            799..883
                     /locus_tag="PC545_003706"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:833..835,aa:Tyr,seq:gta)
     gene            893..966
                     /locus_tag="PC545_003707"
     tRNA            893..966
                     /locus_tag="PC545_003707"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Gly,seq:tcc)
     gene            977..1053
                     /locus_tag="PC545_003708"
     tRNA            977..1053
                     /locus_tag="PC545_003708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1011..1013,aa:Arg,seq:tct)
     gene            1063..1138
                     /locus_tag="PC545_003709"
     tRNA            1063..1138
                     /locus_tag="PC545_003709"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1096..1098,aa:Gln,seq:ttg)
     gene            1150..1225
                     /locus_tag="PC545_003710"
     tRNA            1150..1225
                     /locus_tag="PC545_003710"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1183..1185,aa:Lys,seq:ttt)
     gene            1228..1316
                     /locus_tag="PC545_003711"
     tRNA            1228..1316
                     /locus_tag="PC545_003711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1262..1264,aa:Ser,seq:tga)
     gene            1334..1424
                     /locus_tag="PC545_003712"
     tRNA            1334..1424
                     /locus_tag="PC545_003712"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Ser,seq:gct)
     gene            1433..1509
                     /locus_tag="PC545_003713"
     tRNA            1433..1509
                     /locus_tag="PC545_003713"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1467..1469,aa:Pro,seq:tgg)
     gene            1595..1670
                     /locus_tag="PC545_003714"
     tRNA            1595..1670
                     /locus_tag="PC545_003714"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1628..1630,aa:Trp,seq:cca)
     gene            1731..1807
                     /locus_tag="PC545_003715"
     tRNA            1731..1807
                     /locus_tag="PC545_003715"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1765..1767,aa:Pro,seq:tgg)
     gene            1814..1890
                     /locus_tag="PC545_003716"
     tRNA            1814..1890
                     /locus_tag="PC545_003716"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:gat)
     gene            1894..1969
                     /locus_tag="PC545_003717"
     tRNA            1894..1969
                     /locus_tag="PC545_003717"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1927..1929,aa:Phe,seq:gaa)
     gene            1977..2053
                     /locus_tag="PC545_003718"
     tRNA            1977..2053
                     /locus_tag="PC545_003718"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2011..2013,aa:Met,seq:cat)
BASE COUNT          572 a          480 c          570 g          540 t
ORIGIN      
        1 caacttcaag ataagatttc ccaccgcaag ggtaagaccc cagaaagact atctggttga
       61 taggtcgaag gtgtaagtgc agcaatgtat ttagcttatc gatactaata ggtcgaggac
      121 ttgaccaata ttatttgatg tcattcatta agatatatgt gcagttttta gagtgttaac
      181 tctaaaaaaa aataaaaaaa gtattgacaa aaatagtaaa aaatgttaat attatacttg
      241 tcctagcaac aagaaaaaat gtggttataa tagcggagga gatacacctg tttccattcc
      301 gaacacagaa gttaagttct ctagcgctga tggtacttgg tgggaaactg cctgggagag
      361 taggacatag ccacgtgaat gttccaaggt agctcaacgg tggagcaacc ggctgttaac
      421 cggtaggctg tgggttcgag ccccaccctt ggagccatta tggctcgttg gtcaagaggt
      481 taagacaccg ccctttcacg gcggtaacag gggttcaatt cccctacgag tcaccatttg
      541 tgggatcata gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag
      601 ccctgttggt cccaccattt gcggcctggt agttcagttg gttagaatgc cagcctgtca
      661 cgctggaggt cgagggttcg agccccttcc aggtcgccaa aataaaaatg ctggtgtggc
      721 tcaacggtag agcagctgac ttgtaatcag caggttgtag gttcgattcc tatcaccagc
      781 tccatgtaaa actaatacgg aggatttccc gagcggccaa agggggcaga ctgtaaatct
      841 gttgtcatcg acttcggtgg ttcgaatcca ccatcctcca ccaattaaat atgcgggtgt
      901 agctcaatgg tagagttctg gccttccaag ccagctgtga gggttcgatc cccttcaccc
      961 gctccaaaga aagcgtgggt gcatagctca gctggataga gcaacgccct tctaaggcgt
     1021 gtgtccgggg ttcgaatccc tgtgcgctca ccacatatat attggggatt agccaagtcg
     1081 gtaaggcaca tgactttgac tcatgtatgc gtaggttcga gtcctgcatc cccagccaaa
     1141 taaaaatatg gtctattagc tcagtcggta gagcacctga cttttaatca gggtgtcccg
     1201 cgttcgagtc gcggatagat caccaatgga gaggtgtccg agtggtttaa ggagctggtc
     1261 ttgaaaacca gtgacttcga aaggggccgt gggttcgaat cccaccctct ccgccaaaaa
     1321 aatgactgaa tttggagaag tactcaagtg gctcaagagg atcccctgct aagggattag
     1381 gctgggtaac cggtgcgagg gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt
     1441 agcgcagttt ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca
     1501 cctcgaccaa cttaagcttt tagttcagtt ggttagagtg cccaactcat ggctagtaat
     1561 tctagggttt gagtccatga aggttattct tcataggggt gtagctcagt tggtagagcg
     1621 tcggtctcca aaaccgtgcg ccgggggttc gagtccctcc acccctgcca aataaaaagg
     1681 acttgaaaaa agttggattt tagagtagta taagatagta aataagattt cgaggtgtag
     1741 cgcagtttgg tagcgcacat ggtttgggac catggggccg ggggttcgag tcccttcacc
     1801 tcgaccaaga aaagggccta tagctcaggt ggttagagcg cacgcctgat aagcgtgagg
     1861 tcgctggttc gagtccagct aggcccacca tttgcccaga tagctcagtc ggtagagcag
     1921 gggactgaaa atccccgtgt cggtggttcg attccgcctc tgggcaccag gaatacggcg
     1981 gtatagctta gttggctaga gcgttcggtt catacccgaa aggtcatagg ttcgactcct
     2041 attaccgcta ccatctaact atatcaataa agaactttga aaattaaaca gtaggttaat
     2101 ttataaacaa gaaacaaacc ataaagccag atattttgat aacaatagta tctgagcctg
     2161 at
//
LOCUS       DAMJVI010000222         2133 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.228, whole genome shotgun sequence.
ACCESSION   DAMJVI010000222 DAMJVI010000000
VERSION     DAMJVI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.228"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            258..872
                     /locus_tag="PC545_003719"
     CDS             258..872
                     /locus_tag="PC545_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDF2458407.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTNRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     VGNALRDISKKYPDLVKKELDSWDISNKKVEFVYKLASKCILAK"
     gene            890..1252
                     /locus_tag="PC545_003720"
     CDS             890..1252
                     /locus_tag="PC545_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458408.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYEGYPLKVEYSLTPIRGKQILDALAIMQDIGIS
                     FMLDNGMEDILKEKGVLK"
     gene            1358..1855
                     /locus_tag="PC545_003721"
     CDS             1358..1855
                     /locus_tag="PC545_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HDF2458409.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELNENESVTATDFVQGTTSWHGKLGG
                     LDRLEASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
BASE COUNT          814 a          238 c          354 g          727 t
ORIGIN      
        1 ttctattagt atactcaaat catgtaaaat ttatataact caattaattt tttaaataca
       61 actttataat ataaaactta taataaaagt aaatttatat gttaaatgag gttgattttt
      121 tattattttt tatttatgct agtactaaag acttgcaaac gttttttgac aagtctttat
      181 tttgtctaaa ttaaacaaac ttttttacaa ataaatacat aaaatttaaa ctaatctatt
      241 tattggagga gataaaattg gaagaagtaa ttgcaaaaat tattactata gaaaatggat
      301 tcaaagaaat tgaaaacatt gctaaaaaaa ttgtaaaaaa taatgactca aaaaagtgtt
      361 attatctatc aatctactta tataggtcag aatattatca agtaagagaa ttggctgtgt
      421 ttatacttgg ttttatatca gtaactattt ctgaagcttt gttattttta aaagataatg
      481 tgagtaaaga tgaaaactgg agagtacaag aaatactagc taaatcattt gattattatt
      541 gcagtgaagt aggatatgaa aaggcacttc ctgttataaa agaatggctt aattctgata
      601 gtccaaacat aaaaagggct gtgacagaag gtcttcgaat ttggactaat agagactatt
      661 ttaaatctaa tcctgacgtt gctatatcac tactttctag tttaaaagaa gatgatagcg
      721 aatacttgag aaagtctgta ggtaatgctc ttagagatat aagtaaaaaa tatccagatt
      781 tagtaaaaaa agaattagat tcatgggata tatcaaataa aaaagttgag tttgtatata
      841 agcttgcttc aaaatgtatc ttggcaaagt aaaatggagg tgaacttatt tgaaacttcg
      901 tgatgagtat acttgcccat tagaactaac acatgatatg ataaaaggca aatggaaatc
      961 tattatatta tggagattac gcataggacc aacttcctta tcaaaacttg aaaaggaaat
     1021 caatggtatt aatcaaaaga tgttacttga acaacttaaa gaattaattg aatttggtct
     1081 tgtagataaa aagacatatg aaggttaccc acttaaagtc gaatactcac ttacacctat
     1141 tcgagggaaa caaatattgg atgctttggc tataatgcaa gatataggaa ttagctttat
     1201 gttggataat ggtatggaag atattcttaa ggaaaaaggt gttttaaaat aaatagatac
     1261 ccacaaaaaa gtaagtaatt taccattatt ttcaactggt atataattaa attatagata
     1321 tattctatta atttattata ataggaggta aattattatg aaagtaacta gtacaggtat
     1381 agtaaatggt gttatagaag ataaatatgg taaaagagga actcagttca atgaaggtgg
     1441 tatgcctaca tattcactac ctctaaagtt tgaagatgct ccaaagaata cagtatcctt
     1501 tgctgtattt ttagaagata aagactctgt tcctgtatgt gggtttgcat ggatacattg
     1561 gttagctgta aatataacaa aagatgagtt aaatgaaaat gaaagtgtaa cagctactga
     1621 ttttgtacaa gggactacta gttggcatgg taaactagga ggtcttgaca gattagaggc
     1681 cagtgtatat ggtggaatgg ctcctccaga cagacctcat gtctatgaaa tacatgtatt
     1741 tgcacttgat actatccttg accttgaaaa aggattctat atgaatgaat ttttcaaagc
     1801 tattgaaggg catgttttag atacatttac actaaaagga agttattcta attaattagg
     1861 ttgactatag ctaatcatta attaaatgat acatttaatt gatgattagc ttaattgtta
     1921 ttagaatatt ttctgcactt tatcttaata tatattttag ttgactggta ttttaatttc
     1981 tattagatta ttatcaattt atataaattg attaaaaaat agctacgcac actcgtgact
     2041 ttagtcatga gttagtagct aaaatagtca gcatataggg aaacttatat gtagagatag
     2101 gatagaacct atccaatact acttgaattg cta
//
LOCUS       DAMJVI010000223         2002 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.229, whole genome shotgun sequence.
ACCESSION   DAMJVI010000223 DAMJVI010000000
VERSION     DAMJVI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.229"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            94..492
                     /locus_tag="PC545_003722"
     CDS             94..492
                     /locus_tag="PC545_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458410.1"
                     /translation="MNKKKKLIGIAISFLLIISIINPLQVEAEAIIESSVYISSIKEN
                     YTYDNTKFKTIKSKSRHKSRKYNKSKVKLKKRSIRTKKYGYQTINKYKRQNARRHNIL
                     TKLILFIIVIFLVIVLVVLIFIKRKKKKFY"
     misc_binding    927..1190
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1341..1892
                     /locus_tag="PC545_003723"
     CDS             1341..1892
                     /locus_tag="PC545_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HDF2458411.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNRDDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMNISYNMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLVAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
BASE COUNT          846 a          183 c          300 g          673 t
ORIGIN      
        1 ataataagat atataagaaa cttgaaaaaa tttgtaattt ttagttatat atattataat
       61 gaactagtta aaaattttta tgagggggaa aatatgaata agaagaaaaa attgattggg
      121 atagctatat cttttctact tataatcagc attataaatc cattacaagt agaagcagaa
      181 gctattattg agtctagtgt atacatttct tcaataaaag agaattatac atatgataat
      241 actaaattta aaactattaa atctaaaagt agacataaaa gtaggaaata taataaaagt
      301 aaagtaaagt tgaaaaaacg aagtataaga actaaaaagt atggatatca aactataaat
      361 aaatataaaa gacaaaacgc aagaagacat aatatcctaa caaaattaat attgttcata
      421 atagtcatat ttttggttat agttttagta gtgctaatat ttattaaaag aaagaagaaa
      481 aaattttact aaaaataatt aaaagaatat cattttttta atcattggta tctatttaat
      541 aatatgatgt aattaaaatg gagtaatatt ctgtttcaaa ggaggcatat aaatacagga
      601 atgagtatta aatgatggtt aattaagtaa atttattgat aaatgtactt aattaacttt
      661 gaagaattta attcctcatg aatcaaaata taaaattaca tcaacagtta ttcacaaaat
      721 taatataaga agaattagag ccaaagagca gactattaga ccattaaagt aattattcat
      781 agaattttgt atatgaaaag aaatgtatag tataatttac tttttttata aaaaatagat
      841 tacaaaatat attttttata aaaaggtatt gacatttttt agaaaaaaga atattattat
      901 tttaaaacaa aattcaatac aataaattaa atcctatgac aaggaagagt agttaaattt
      961 aggttcagta tagagagctg aggatggtgg aatcttagca agaaaaaatt tgatgaatgg
     1021 cccttagagg aataaattga aatcatagtt ttgagagtag actttatcgt cagtcgcacg
     1081 ttatagcgaa taaggtatgt aagtacctga taatgagatg tatgaaaatt gaattttata
     1141 ttaattttta tataatttag gtggcaacgc ggataatctc cgtcctatta gtttaggacg
     1201 gagtttttta ttttactgaa aatttaaaag atagaaggtg gtgagaaaga tgttatgaat
     1261 ttcaaaagct aaagatgatg atatagtagg ttggcaaaaa aataaattga aaattaaaaa
     1321 aataggagga agtacaaaaa atgaaaacaa atacaaaaaa attaacttta aatgcaatac
     1381 ttttagcgat gggattatta atacatcaaa tcacaccagc tataggatta ccaatgaagc
     1441 cagatgtacc actagcaatg ttatttgtaa tattggtttt aaatagagat gattataaaa
     1501 cttgtttgat agcgggtata gtaacaggaa tatttacagc acttacatca agttttccgg
     1561 gaggtcaaat tcctaatgta atagataaaa ctcttacagc aaatatagta tttttattaa
     1621 tgaatattag ttataatatg ccttttataa aaaaattagc taagaaaaca caagatactg
     1681 tagtagttgc aataataatg ccaataggaa ctcttgtaag tggaactatc tttttattag
     1741 tagctcaagc tatagttggt cttccagggg cttcatttac agctttattc ttagcagtag
     1801 tattaccagc tgttttaata aatctagttg caggaatatt tttattcaag attgttagtc
     1861 tatcaataag aagagttagt tatcaagctt aaacacgaaa taatatttta ctttataaag
     1921 cattataata actaaataac ataagaataa agtagattat aactatttta ttcttatgtt
     1981 gtttaaagat ttggtattat at
//
LOCUS       DAMJVI010000224         1955 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.230, whole genome shotgun sequence.
ACCESSION   DAMJVI010000224 DAMJVI010000000
VERSION     DAMJVI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.230"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            195..479
                     /locus_tag="PC545_003724"
     CDS             195..479
                     /locus_tag="PC545_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458412.1"
                     /translation="MLVYNKSFYPNDIFPRLDFSKIKKQLKLIDNDLSDFGSICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYRIVYEIEKLFKSQVGRFSISTYRN"
     gene            complement(636..809)
                     /locus_tag="PC545_003725"
     CDS             complement(636..809)
                     /locus_tag="PC545_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HDF2458413.1"
                     /translation="MKRKLSTYFKGYILGLCIAFLLFTFILKKLIIENIIGIIIGTGI
                     AFLLIYLFNKKRK"
     gene            complement(1250..1465)
                     /locus_tag="PC545_003726"
     CDS             complement(1250..1465)
                     /locus_tag="PC545_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDF2458414.1"
                     /translation="MAIVVNLDVMMAKRKISLKELAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDEND"
     gene            complement(1476..1943)
                     /locus_tag="PC545_003727"
     CDS             complement(1476..1943)
                     /locus_tag="PC545_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2458415.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALIARILYFSDYFIVILFLIFIINSTKYSPFVLENVKRFKVMGFCLLINTIIECING
                     YNLNTNNIRIFGTDSGGISPLMVISFIFALMCFVIAETFDKAIKIKEDNDLTI"
BASE COUNT          794 a          204 c          239 g          718 t
ORIGIN      
        1 atttttttat tgttgtggat gaaaatatac ttatatttta ctaatataca tttaaaaatt
       61 acaatttaaa gcattaaaaa ataaatttgt attttgtttt atataaagta atttgaaaaa
      121 tatggaaaaa ttgattagta cattattaat tagggtataa aatggtgtaa atagaatgat
      181 aaggggtgga tgaaatgctt gtatataata aaagttttta tcctaatgac atatttccaa
      241 gattagattt ttcaaaaata aaaaaacagt taaaattgat agataatgac ctgtcagatt
      301 ttggaagcat atgtataata gaaaaagaac attatacgat aagtgtaaac agtataggtg
      361 aaataaatgt gtactatgat ttagagtacg aaaataaggt gtatagaata gtttatgaga
      421 ttgaaaagtt atttaaatct caagttggaa ggtttagcat atctacatac agaaattgat
      481 aattaaaaaa gtagaaatta aaaaacttaa tactaaagat atagataaat atatgaggta
      541 acagctagag agagttgtta ccctttttaa atactaagta tatattattc ttattaattt
      601 tttgtaaatt cactaaaaga cctttttata gaatattatt ttcttttttt attaaatagg
      661 taaatcagta aaaatgcaat tccagttcca attattattc caatgatatt ttctataatt
      721 aattttttta atataaaagt aaataataaa aatgctatac ataaacctaa aatatatcct
      781 ttaaaatagg ttgaaagttt tcttttcata taatcaccaa cttgtatcag atttagttta
      841 cttaattaga gtataacagt gttgtaactt caaagagtat ctaaaagatt acaaatgtta
      901 gataaaatca tcaataatgt aatttataac acatagctat aagctatgca ataatataaa
      961 tggaggtata agtaaatgaa aaagttccat gttttgctaa cagtttaaat aaatattatg
     1021 tagaaaagaa tgtatatagg aatctgtggt aaaattatca atttaaaagt attatttagt
     1081 gtgatacagt tcttataggt aatggataac aaatagataa aggaacagtt tctttcttag
     1141 aacatatttt gttatgactt taaacaaagt taatattaat gtcaataata tagtaaaaga
     1201 ataaaaatca tatagtataa taaaaaagag tgtttctaaa cactcttttt taatcatttt
     1261 catcttctac atattcaagt atatcacctg gctgacagtc taaagctttg caaatttcgt
     1321 ttagagtggt aaatctaata gcttttgctt ttccagtttt taaaattgat aaatttgcat
     1381 ttgtaatatc tattttttct gccaattctt taagtgatat ttttcttttt gccatcatta
     1441 catctaggtt aactactatt gccataacat cacctctata tcgttaaatc attatcttct
     1501 tttattttta tagctttatc aaatgtttct gcaattacaa agcacattag tgcaaatata
     1561 aaagagataa ccataagtgg tgagataccg ccactatcag ttccaaaaat tcttatatta
     1621 ttagtattta aattataacc attgatacat tcaattatag tgtttatcaa taaacaaaat
     1681 cccattactt taaacctttt tacattttca agtacgaatg gggaatactt agtactatta
     1741 attataaata tcaagaataa gatgactata aagtagtcag aaaaatatag tattcttgca
     1801 attaaagcaa ttactttgtc agaactaaaa tcatataaaa acataggtat catacataag
     1861 aatattatac cagcaactcc taaaagtgat aatagaaaat atagaaatac tgataatata
     1921 ttactaaaaa attttgtaat cataaaatat catct
//
LOCUS       DAMJVI010000225         1876 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.231, whole genome shotgun sequence.
ACCESSION   DAMJVI010000225 DAMJVI010000000
VERSION     DAMJVI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.231"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            144..338
                     /locus_tag="PC545_003728"
     CDS             144..338
                     /locus_tag="PC545_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDF2458416.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(440..949)
                     /gene="vanZ1"
                     /locus_tag="PC545_003729"
     CDS             complement(440..949)
                     /gene="vanZ1"
                     /locus_tag="PC545_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HDF2458417.1"
                     /translation="MKSRKHNITKGLFIVYIIILTWIILFKLQFDISSLETMNLRSIN
                     LVPFAGSLIINNRVDISEIILNVAIFVPFGIYVCMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINVLALIVTIIVVLF
                     LGLVIFANL"
     gene            complement(1269..1658)
                     /locus_tag="PC545_003730"
     CDS             complement(1269..1658)
                     /locus_tag="PC545_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HDF2458418.1"
                     /translation="MKINKLPHAELKLMKYIWGVDDVLASRDIIEDMKLKYDWKKSTT
                     LTFLKNLVDKGFLTTDKVDRCTHYTIAIKEKDYLKVETKSFFSFMHNNSFKSFISALH
                     DDEVLDSKSLDKLEEYFKNLKEGDIDD"
BASE COUNT          694 a          260 c          190 g          732 t
ORIGIN      
        1 ttacctataa cctttatttt tttattaaaa tgcattataa taaaaagtaa atctaaattt
       61 gatacagttt taacaatcat tattttcaga aaagtgatac tattgaatat attaagaata
      121 attagagatg gaggaactaa gatatgagcg taattgtaat gtgtagtcgt tgtggaaaag
      181 aaatagataa atatagaaat aaatatataa cttattatga gaaaaatacc aatgcagaaa
      241 ttaaagtttg tataaattgt gcgttagatt tattagaaaa aacaagagaa aatcaagaat
      301 tgaacataca agagtttcaa gatacaataa ttctttaata atttaggaaa gcacctgtat
      361 aggtgctttt tatgtgtaaa taattttata tgtaactgta tcttacgttc tattttaagc
      421 taaacatata tacttatttc tataagtttg caaaaataac taatcctaaa aataatacaa
      481 caataattgt cacaatcaat gccaatacat tgataatttt tatggtatta ttcttaaata
      541 ttttcgataa tagcataaaa acagctattc ctatgattcc tcctagtgtg tttcctatca
      601 aatccgttat atcacttgca cctagtgcaa atatatattg aagtgtttca aatgccaaac
      661 tagttataaa aataggtata acttttttta taaagctcca ttcttctttt agcatacaca
      721 catatatccc aaatggtaca aatatagcca catttaatat aatctctgat atatctactc
      781 tattattaat tataagtgac ccagcaaaag gtactaagtt tatgcttcta agattcatgg
      841 tttcaaggga tgaaatatca aattgcaact tgaatagtat tatccatgtt aaaataatta
      901 tgtaaactat aaataatcct tttgtaatat tatgttttct agatttcatt tattacctcc
      961 ttactatatc attgtattat tttattaata caatgatata gtaaggtaat ttatctgtca
     1021 actatttctt ctaaaattat attcctttta taatttcaat atctttatca tatttacatg
     1081 atttaagcta ttgtagtatt tcatatatct ttaaatttta attggtatta ttctctatat
     1141 tttttactat caaacactaa ggaaataatc acttttcttt atataatgtt ctaccatcac
     1201 ataaaactaa cattaaatca aaacgctact ctcaattgtc tcaaactaaa aagatactga
     1261 taactttatt agtcatcaat atctccttct ttcaaatttt taaagtattc ttctaacttg
     1321 tccaagctct tactatctaa aacttcatca tcatgtaatg ctgaaataaa actcttaaat
     1381 gaattgttat gcataaaact aaaaaaactc tttgtctcaa cttttaagta atctttctct
     1441 tttatagcta tagtataatg cgtacatcta tctaccttat cagtagttaa aaagccctta
     1501 tctactaaat tttttaaaaa tgtcaatgtt gtgctctttt tccaatcgta tttcaacttc
     1561 atgtcttcta tgatatctct tgaggctaat acatcatcta ctccccaaat atacttcatt
     1621 agttttagtt ctgcatgtgg taatttatta atcttcatta gtttacacct cttaccaatt
     1681 tatgtataac taaactttat aacaataagt gtatataatc aattagattg gaataaatat
     1741 aaattccaat ttattctata taaaaataat taataaaaaa gaagtactct actttagaga
     1801 gtacttttgg attatatatt caaaaattta tctaacatga ttagagtatg ttttatgctt
     1861 tagtgtgata atttta
//
LOCUS       DAMJVI010000226         1823 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.232, whole genome shotgun sequence.
ACCESSION   DAMJVI010000226 DAMJVI010000000
VERSION     DAMJVI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.232"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(284..1612)
                     /locus_tag="PC545_003731"
     CDS             complement(284..1612)
                     /locus_tag="PC545_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDF2458419.1"
                     /translation="MGSNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMVVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPANELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYGIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGVKR"
BASE COUNT          667 a          331 c          208 g          617 t
ORIGIN      
        1 ataaaagcta tgtataatat ttgttgtaca gataccactt agtttgataa aaaacacttt
       61 aaaataaagt attttaaaat aaataatttt ttagatgttt aattttataa tcaaaaaact
      121 aaaaataaaa tgaatacctt aaaataaact ttttagttca tggaacgctc tttttttgta
      181 aaattaaaca tatctttacc attttaacaa taaaaaagag acttatctct atttgagaca
      241 gtctctttta ttttaattat gaagcatttg taaactactt ggtttatctt tttactcctc
      301 tagtcatata agcatacact ggtaagccta tagctgttat tacaagacct cctatagata
      361 tcatagtagt tttcatacct gcaaaacaca attgattaac tacaacaaat ataccacttg
      421 ctatagctat tataggaaca ataggatata atggaacttt atactctctt ggtatatttg
      481 gatgagtctt tctaagtttc ataaccccta taaaagttaa tacatagaat atccaagtag
      541 caaatacagc taagtcagtt aataaattaa attgacctga caaagcatat atagatgcaa
      601 taacagatac taatgctatt gcatttgctg gaacttgagc agaatttaat ttagaaaata
      661 taccatactt tggtaaagtc ttatctgtag ctaaagtata tgccaccctt gaaccagtta
      721 gtaaaaatcc atttaatgct ccaaacactg aaattaatat acctacagat attatttttc
      781 cacccattga accaaatata acttcagcaa ctgctgatgc aggagaagca taatttgcta
      841 attcattagc tggtaatacc caaagatacg ctaaatttat tataaaataa acagccataa
      901 caactgacaa tccacctatt atagcttttg gaaggtcttt tccaggattc ttcatttctc
      961 cagctaaagt accaacattc atccatccat caaaagcaaa taatatagca attaaaactt
     1021 gacctagtac actacccagg cttaatcctt caccaaccat aggtgtaagt attggattat
     1081 ttccaccacc tttaataaat ccaacaacca ttattaaaat aagtggtatt aacttacaaa
     1141 ttgtagaaac agtttgtatg actcctccag tttttgaacc taacatatta agccctgcaa
     1201 ctattactat aattccaatt gctattggaa gtaaaagtga tggactacca attagtgctg
     1261 atgcttgttc tccaaatata acacctaatg ctgccatcat ccctggataa aataatacaa
     1321 tttgcatcca tccagttaaa aaacctaatt tttctccata aatttctttt atatatacca
     1381 tcattccacc agtttttggt atcgcaactg atacctctgc agctgtaaga ccagctgtta
     1441 tagttatgat tccagctatt aaccatgcca atataccaag acctggagct cctccagtta
     1501 atgtatagac tgcttgtggt ttaaaaaata cgcccgctcc tataacggaa ccaacaacag
     1561 ttgataatgc tgctgatacg ccaagagtct ttttcagttc attacttccc atttttttcc
     1621 tcctcattaa tcctatttaa ttttaatttc atagttaaat aagcatatat tatgccaata
     1681 tatagaaaaa tagctttatt atttattaaa ataaaataaa ctctacttag ctttcgtaat
     1741 cttttatttt tagctatgtt taaatataaa aaaattataa aaaccaaaaa tccaaatata
     1801 ataatacttc taagtaaatg tat
//
LOCUS       DAMJVI010000227         1823 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.233, whole genome shotgun sequence.
ACCESSION   DAMJVI010000227 DAMJVI010000000
VERSION     DAMJVI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.233"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            94..663
                     /gene="mocA"
                     /locus_tag="PC545_003732"
     CDS             94..663
                     /gene="mocA"
                     /locus_tag="PC545_003732"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDF2458420.1"
                     /translation="MINAVIMASGFARRMGANKLLLNYKGCSIIEHVFREVSKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANIGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDMCGNPVIFPYSKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKMDYERIGGKL"
     gene            660..1679
                     /locus_tag="PC545_003733"
     CDS             660..1679
                     /locus_tag="PC545_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HDF2458421.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKEII
                     KSAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
BASE COUNT          730 a          164 c          347 g          582 t
ORIGIN      
        1 tttatatgat attatatata tagataatat caatagagat attataaaaa tcatagaact
       61 atcaatatat tataaatacc aaggatgata tatatgataa atgcagtgat aatggcatca
      121 ggctttgcca gaagaatggg agcaaataaa cttttattaa attataaagg ctgttctata
      181 atagaacatg tatttagaga agttagcaag attgattttc atcaagttgt agtagtaagt
      241 caatatgaag aggttttgtt attgtgtaat aaatatggtt ttgaatatat agataataaa
      301 aatgcaaata taggtcaaag tgaatctata aaattaggag ttttaaatag ctcaaaatgt
      361 gatggttata tgttctttgt aggagaccag ccatttattg ataacttata tatagataaa
      421 attataaata cttttaaatt agatagagat tttatagtaa ttccaagata caaagatatg
      481 tgtggaaatc cagtcatatt cccatatagt aagaaagaag aattgttggc acttaaagaa
      541 gatgaaaaag gtaaaaccat tttaaaaaat tcatcaaaaa taaagtatgt agaagttcct
      601 aaagaaatgt tatttgatat cgatacaaaa atggattatg agagaattgg gggtaaatta
      661 tgaagctaat aaaaactata gatgctgttg gacaagtttt atgtcacgac ataactcaaa
      721 taatacctgg agaatttaaa ggaagaaaat ttaaaaaggg ccacatagta aaagaagaag
      781 atattccagt gttattatct cttggtaaag ataatttgta tgtatgggaa aagtcagaag
      841 gaatggttca tgaaaatgaa ggcgcattgt ttttaaaaga actaactgct ggagaaaatt
      901 tagattttag tgaaataaaa gaagggaaaa tagattttat agcagcatgt gatggtctat
      961 taaagataga tgttgatgca ctttttgatt tgaattgtgt tggagaaata atgatggcta
     1021 ctcttcataa taattttata gtaaataaag gtttaaaagt agctggaaca agagtgatac
     1081 ctttagttat agatgagaaa aaattagaag atgcaaaaaa agtagttggt aatagaaaaa
     1141 tagtaaatgt agtacctttt aaacctaaga aagtaggaat tgttactaca ggaaatgaag
     1201 tgttttatag cagaattgtt gataaatttg gacccgtaat agaagaaaaa gtaaaaggct
     1261 ttggttgtga agttatagga cagactatat gcccagatga taaagaaatt ataaagagtg
     1321 caataaaaga atttataagt caaggagcag aacttatctg ttgtactggt gggatgagtg
     1381 tagacccaga tgatgttaca ccaacagcta taaaagaaac tggagctgat ttagtgacat
     1441 atggttcacc aatattacca ggagctatgt ttttacttgc ttattatgga gaagttccaa
     1501 ttatgggtat accaggatgc gcaatgtatc acaaaactac agtatttgat atagttttat
     1561 ctagggtttt aattgatgaa aagcttgata agtatgatat agctagatat ggtcatggtg
     1621 gcctttgtat gaactgtgat gtatgtactt atcctgcatg taattttgct aaaatataaa
     1681 gttattaaaa aagagtattt acaaattaag tactcttttt aaatatgaaa caaaacagct
     1741 agaatgatgt tttaagaaaa gcatatttat ttagaaatta taataactta ttttcaatgt
     1801 gtaaaagaat gaataaagtt gca
//
LOCUS       DAMJVI010000228         1822 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.234, whole genome shotgun sequence.
ACCESSION   DAMJVI010000228 DAMJVI010000000
VERSION     DAMJVI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.234"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            99..1445
                     /gene="rpoN"
                     /locus_tag="PC545_003734"
     CDS             99..1445
                     /gene="rpoN"
                     /locus_tag="PC545_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDF2458422.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT          839 a          161 c          321 g          501 t
ORIGIN      
        1 cccttttgca taaggtatat tttaaatgat agaatataca tatacaggag ggtttttctt
       61 ttataaaata agtttaataa aattgagagg tgagcagaat gaattttaat aatagtctag
      121 aattaactca gtctcagaag ctaataatga ctacacaact taagcaatca ttatcaatat
      181 taaatatgag taagttagag ttagaagaag agataaaaag agaagcagag aataacccat
      241 tagttgaagt cgagaaaagt ggggagataa attgggaaga gtatataaaa gacatggata
      301 ggtcaagacg tttagataga acagagataa gctataatcc tgataatgaa gttaatctag
      361 aaaatttagt taaatattcg tcaaatttat atgaagattt aaaatttcag ataagcctgt
      421 ataaacttac agacaaagaa ttggaagtct gtgagtatat aattgatagt ttagatgagg
      481 atggatattt aagaactgag gaaaaagtaa ttgttgatac cttaaagata gatgaagaac
      541 tttttgaaaa atgtttaatt agtgttcagc agttggaacc tagtggagta ggggcaagaa
      601 gtctttcaga atgtctgatg atacaaatgg ctagtttagg aatatataat gaaattttag
      661 aggaaatcgt tacaaaagac ttaaatttaa ttgcaaataa taaatacaaa gagataagta
      721 aaaagtacaa tatgtcatta caaaaatgtg ttgaactaat aaacataatt aagacattag
      781 acccaaaacc aggaagaact tgttcagtag agaaaagtgt atacattcaa cctgatgtaa
      841 cagtagaaaa aattgatgat gaatttattg tatatttaaa tgaaaaagat tcttatcaaa
      901 taagaatcaa tagttattat aaagacatat taaaaaattc acagtctgat gaaagtgcaa
      961 aagaatttat aaaagaaaga ctaaattcag caacaggact tatgaaaaat atagagagta
     1021 ggaaaactac agtacttaag attgcagaag agatagtaaa agctcaagat gaatttttga
     1081 gaaaagggac aaaatatata aaacctttga aaatgaaaga catagcagaa aaattagaat
     1141 ttcatgaatc tactataagt agaggtgtaa atggtaaata tatgttaaca ccttttggtg
     1201 tatatgaatt tagatacttt ttcagtagtg ccattgagac tgagaataat gaaatggcat
     1261 ctagtacaag cataaaaaaa attataaagg aaacaataaa agacgaaaat aaaaagaaac
     1321 ctttaagtga tgaccatata tctaagttac taaaagaaaa aggaatcaat gttgcaagaa
     1381 gaacagttgc caaatatcgt gaagaattag gaatcccatc ttcaagtaag agaaaagaat
     1441 actaataagt aaaagacaaa tatcagtatg gtaaggggaa aaggctaatt aaaagatgaa
     1501 tgtagattgg gctaaaaatt ttatgaagta aaataataga atatggaaaa gtattaaaat
     1561 gctataagat attaaaaaac tggggaacat taaaacatta agatagcaaa gaacataaaa
     1621 aactaaagaa gattaaaaca ttaaagaaca ttagagcttt gaggaatatt agaactttaa
     1681 agaatattaa aatttataaa atataaaaat actaaaaaga attgaaaaac tggggaacat
     1741 taaaatattg taaaatgcta aaaaactaga aaacataatt aaataagtgc ttataaagaa
     1801 tgattaaatt aaataagtgt tt
//
LOCUS       DAMJVI010000229         1816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.235, whole genome shotgun sequence.
ACCESSION   DAMJVI010000229 DAMJVI010000000
VERSION     DAMJVI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.235"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            227..1039
                     /locus_tag="PC545_003735"
     CDS             227..1039
                     /locus_tag="PC545_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HDF2458423.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNGVTEISDDLDSILTNVVEKIK"
     gene            complement(1128..1319)
                     /locus_tag="PC545_003736"
     CDS             complement(1128..1319)
                     /locus_tag="PC545_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458424.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTQPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            1590..>1816
                     /locus_tag="PC545_003737"
     CDS             1590..>1816
                     /locus_tag="PC545_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458425.1"
                     /translation="MNYNLIKVIFSLFKKKSGYTFTNFLNKVRVEKSKKYILKKDLSI
                     LDVAVLIGFNSQNYYSIIFKKFNNFTPIEYK"
BASE COUNT          684 a          206 c          245 g          681 t
ORIGIN      
        1 tttctatata tttatatgac taagttctat aactaaattg aaataatcac tttttcaaga
       61 atgttataat ttttttattg tattcttttt gctatagtct atgaataaat caatagaaaa
      121 tcttctaagt tgcttttaaa taatgacttt atggttcaat tgtactaaaa tagtatatgt
      181 aggttacaaa taatctaata aatattaaag tttggaggta atagatatgg cagattgtaa
      241 ttcatgtcca tcaaagggaa attgtaatag tcaatcaaat tgttctattg aaaataaccc
      301 taacaataaa tttggaaaaa taattggtgt tatgagtggg aagggtggag ttggaaaatc
      361 aactgtaact gctttacttg ctaataagct aaacaaaatg ggatataagg ttggtatatt
      421 agactcagat ataactggac caagtattcc tagacttatg ggtgtaaaaa atgtaaaagc
      481 ttattctgat ggctcttata tttaccctgt agaaaactca aataacataa aagtaatgtc
      541 tataaacctt atgatagatg atgaaaatga acctgttgtt tggagaggac ctttacttgg
      601 aggggttgta aagcaatttt atactgatgt actttgggaa gaattagact atttattaat
      661 agatatgcct cctggtactg gagatgttgc cttaacagtt atgcaatcta ttcctataag
      721 tggaatagtt atggtttcag tacctcaaga tttggtatct atgattgtat caaaagctgt
      781 taatatggct aaaaaaatga acataaatgt ccttggagtt attgaaaata tgagttatat
      841 ccaatgtcct gattgttcta aaaaaatcaa gttatttgaa ggagaatcta ctgaaaagtt
      901 tttagatgat ttagacttag agcttttagg agaacttcct atgactaaag aaattataga
      961 tattactcat aatggagtta cagaaattag tgatgattta gattcaatat tgactaatgt
     1021 tgttgaaaaa attaaataaa taaaaatttt ataattttat atttatcaaa aaaagaggct
     1081 tgtatttatt ttataatcat acaaataaat ataaacctct tttatgttta tctatgtttt
     1141 aaatacacaa aatattcttc tacttcatct ccatttttta cttttactaa atattcatta
     1201 taatgagcat tatatggctg tgtcattttg cattctatta cattttctat gtcttctaat
     1261 tctaaaagtt ctacatcctt taaaaatttt ctttctatga gttccattaa tctttccata
     1321 atatatcctc acttccccta taattaagat aaactatata tcctcttaat gcagagttta
     1381 tctgtttatt atattatatc caatatatta cagtttctaa catcattcat aaattatttt
     1441 aacaaataga gattgatttt ttaataaata tgccttgata aaatgaaaca tagaccaact
     1501 cttagtttta tgtaaagaaa acttaaagaa aactataaat tatattcaca aaaattactc
     1561 agaagatata acagtaagta atatatgtaa tgaattacaa cttaataaaa gttatttttt
     1621 ctttgtttaa gaaaaaaagt ggatatactt ttactaattt tttaaataaa gttagagttg
     1681 aaaaaagcaa aaagtatata ttaaaaaaag acttgtctat tttagacgtt gctgtactaa
     1741 ttggattcaa tagtcaaaat tactatagta taatttttaa aaagtttaat aactttacac
     1801 caattgaata taaaaa
//
LOCUS       DAMJVI010000230         1807 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.236, whole genome shotgun sequence.
ACCESSION   DAMJVI010000230 DAMJVI010000000
VERSION     DAMJVI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.236"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            101..448
                     /locus_tag="PC545_003738"
     CDS             101..448
                     /locus_tag="PC545_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2458426.1"
                     /translation="MHENKKNLLDGSLAQYNDTAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(598..1647)
                     /locus_tag="PC545_003739"
     CDS             complement(598..1647)
                     /locus_tag="PC545_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDF2458427.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWIQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
BASE COUNT          611 a          280 c          247 g          669 t
ORIGIN      
        1 ataaaagaat tttattccta ttgtgtaaag tgagaatact attttcttat attggtataa
       61 taatagtaaa tctataatat agaaaagagg tatttctagt atgcatgaaa ataaaaaaaa
      121 tctattagac ggaagcttag ctcaatataa tgatacagca gttccaaaaa taccagtatt
      181 tgctggtaac gatataactt cagaagttta ttattttaaa cctaatcaag ttcttaatgc
      241 tcatagacac cctaatggtg aacaaatatt tgtgttccta aaaggagaag gtaaaatgaa
      301 acttggtgag catgaatgtg atgtaaaaaa cggagatact gtatttgtac caacaggaga
      361 gtggcatgaa attactaatg gaagcaatga agaaatggta gccgttcaaa ttacaaaaat
      421 taatgctgga gcagaataca gaggatagat atataagtac agttagtact ttaatatcta
      481 ttttattata taaactttaa tctcaaatat aaaaagagtc ttttaaaagg ctctttttat
      541 atttttgtta attttcatta taaaaacaag aaaatatcta gctaaaacta atatatttta
      601 tttaatattt tttatagcat tgtttgcaaa ctcagtattt acgatatctt tataaggtgc
      661 ttccttatct agttctttag cttctttcat aacagtcatc aatttcttca aaccttcctc
      721 ttttaataca ggattttgag accatgcatc tattgattta tatctatcta caactgttgt
      781 taaatcttct aaagatatat cttcaaagaa aggttgcata gccttagcta cttctttgct
      841 agatgcttgt tgaatccata attgaccttt ataaacagca tttgtaaatc tttgaattaa
      901 atctttattg tttttcatgt atgatctcgt agtagagtat gctgtaaaag gtatttctcc
      961 agaatctttt cctatagatg taactatata tccactacct tctttttcca ttgctgttgc
     1021 tgtaggttca aatgctgttg taaagtcacc ctctccacct gcaaaagctc cagccattac
     1081 accaaattgt acatcagttc ttacattaac attgcctgct tttagatttt ctccaatagt
     1141 aagacctttt tcttttagaa catattcaaa agtcattagt ggaactccac cttttctacc
     1201 acctaatatt tcggttcctt ttaaatcatt gtaagagaaa ttaggcattt ttttcctagc
     1261 aactagaaaa ctaccatctc tttttgtcaa ttgagcaaag ttcactgcat aatcgttttt
     1321 gttttgatta tatacatata tagatgcttc aggacccatt aaacctacgt ttgcttcacc
     1381 tgaaatcaaa gctgccatag ttttatctgc accttgggta tttataaggt caatctttat
     1441 accttcatct tcaaagaatc ctttcgtaat agcagcatac tgtggagcat aaaaaacaga
     1501 gtgagtaact tctgctatag ttactttatc cagattttta gacgaagaag attcatcttt
     1561 tttgtcatta gagttaaaat cacatccagt tagacatatt gtacttacaa tgatagagct
     1621 tgctattgcg actatttttt taaacataat aaatacctcc cataagacat acgaatagta
     1681 tatagtatta aatatttatt gtagttgtta caaaaatgta tctatactta taatatttct
     1741 acaaaaactt aataataatt ttcagtttta tgtttagttt tttcagtaat gtgtaatatt
     1801 ggataaa
//
LOCUS       DAMJVI010000231         1805 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.237, whole genome shotgun sequence.
ACCESSION   DAMJVI010000231 DAMJVI010000000
VERSION     DAMJVI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.237"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..92)
                     /locus_tag="PC545_003740"
     CDS             complement(<1..92)
                     /locus_tag="PC545_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458428.1"
                     /translation="MKDKWILCPVCGNKTRLKIRADTELKNFPL"
     gene            complement(131..418)
                     /locus_tag="PC545_003741"
     CDS             complement(131..418)
                     /locus_tag="PC545_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015574378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458429.1"
                     /translation="MSTLIIGFIVAGVLLIAEYLLCTKFRSPLWGGIIPLLILAGTIY
                     VFASGRIPFERPYIFPFVVVNTLFFGDWGTGRDKYKKIKQTEMEKMKAKDI"
     gene            complement(421..717)
                     /locus_tag="PC545_003742"
     CDS             complement(421..717)
                     /locus_tag="PC545_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HDF2458430.1"
                     /translation="MELDKIPQWILALEQEDATFLKNFALKSGSLKEIAKMYGVSYPT
                     VRLRLDKLIQKIEMCDQQEEEPFQTFIKGLAVDSRIDVETAKIIIEKYKKEKEK"
     gene            complement(806..1711)
                     /locus_tag="PC545_003743"
     CDS             complement(806..1711)
                     /locus_tag="PC545_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDF2458431.1"
                     /translation="MFKKNKKQTENVKEKKVRTVKVGTHKKSVIALWVVLIASVSFGV
                     YKNFTAIDMHTVHETETIQLRLTDTNGIENFVKNFAKSYYTWNNSKEAIEARTQAISG
                     YLTKELQALNTDTIRKDIPVSSAVNGVQIWSVTQADENQFSVIFTVEQLITENGSTKT
                     VRSAYEVTVYVDGSGNMVLTKNPTITSIPVKSGYTPKAKESDGTVDAVTTDEINEFLT
                     TFFKLYPTATAKELSYYVNEGVLKPIGKDYVFSELVNPIYNRIDSQVTVSVAVKYLDQ
                     QTKATQVSQFDLVLEKSGSNWKIIK"
BASE COUNT          485 a          397 c          300 g          623 t
ORIGIN      
        1 aatagtggga aatttttaag ctctgtgtcc gctctaattt tcaacctcgt tttattaccg
       61 caaactggac ataaaatcca tttatctttc aataatgcgt tcctcctatc taagtataca
      121 gttttgtaat ttatatatct ttagctttca tcttttccat ttcagtttgc tttatctttt
      181 tatatttatc cctgccagta ccccaatctc caaaaaataa agtattgact acaacaaacg
      241 ggaaaatata tggtctttca aatggtattc tgccacttgc aaaaacatat attgttcctg
      301 ccaagataag caagggaata atacctcccc aaagaggact tctaaatttt gtgcagagaa
      361 gatactctgc aattaataaa actcctgcaa caataaagcc aataattaat gtactcatgg
      421 ttatttctcc ttttcttttt tatatttttc aataattatc tttgcagttt ccacgtcaat
      481 tcgagaatca accgctaacc ctttaataaa agtttggaaa ggttcttctt cctgctggtc
      541 gcacatttca attttttgaa ttaatttgtc taaacgtaat cgaacagtag ggtaagatac
      601 cccatacatt ttcgctattt cctttaatga accggattta agtgcgaagt ttttaagaaa
      661 agtagcgtcc tcttgctcta aggcaagaat ccattgaggg attttatcaa gctccaaaaa
      721 cacaactcct ttcatattat ctttaacaat attcagtata tcataaataa aattaaagtc
      781 aataagaatt taaacatcaa aataattatt ttataatctt ccagttgctc ccgctttttt
      841 ccagcaccaa atcaaattgt gatacctgcg ttgcctttgt ctgctggtcg aggtacttca
      901 cagccacgga aaccgtcact tgactgtcga tgcggttgta gatgggattt accagttccg
      961 agaatacata atctttcccg ataggcttta acacgccctc atttacataa taggacagct
     1021 ctttagcggt tgcggttggg tagagcttaa agaacgtcgt cagaaattcg ttgatttcgt
     1081 ccgtcgtcac agcgtccacc gttccgtcgc tttcctttgc ctttggcgtg tagccggatt
     1141 tcacgggtat gctggtaatg gtcggattct tcgtgagaac catgtttcca gagccatcca
     1201 catagaccgt gacttcatag gcggagcgta ccgtcttcgt gcttccattc tccgtgatta
     1261 actgttccac agtgaagatc acggagaact ggttttcatc cgcctgcgtg acagaccata
     1321 tctgtacccc attgaccgcc gaagacactg gaatatcctt gcggatggta tcggtattaa
     1381 gtgcctgcaa ttcctttgtc agataaccgc tgattgcctg cgtccttgct tcaatagctt
     1441 ctttgctgtt attccatgtg taataggact tcgcaaagtt cttcacgaaa ttttcaatcc
     1501 cgttggtatc ggtaagtctt aactgtatgg tttctgtttc atgtacggtg tgcatatcaa
     1561 tagcggtaaa attcttatat accccaaaac tcacgcttgc gataagcact acccacaatg
     1621 caatcacaga ttttttatgt gtaccgactt tcacagtacg cactttcttt tctttgacat
     1681 tctctgtctg tttcttattc ttcttaaaca tattttctaa gtcctttctt ttattttact
     1741 cgtcctgcac cgattaagtg ttgttgccag taactattat ttaagtctgc atatcctatc
     1801 ggatt
//
LOCUS       DAMJVI010000232         1786 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.238, whole genome shotgun sequence.
ACCESSION   DAMJVI010000232 DAMJVI010000000
VERSION     DAMJVI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.238"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            56..1561
                     /gene="ade"
                     /locus_tag="PC545_003744"
     CDS             56..1561
                     /gene="ade"
                     /locus_tag="PC545_003744"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDF2458432.1"
                     /translation="MSPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADPHELVNV
                     KGVSAIDFLLESTKDIPLNVYIMVPSSVPATSFETNGVGKFSAKDMESYVNNPRILGL
                     GEVMCFNDVINSENEILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDHECITFD
                     EVYEKLRAGLKILIREGSAAKNLKSIVSGMLKHNLPIEEFMFCTDDKHLDDIEKQGHI
                     RWNIKCAIDLGMEPVRAIKVATYNSAKAYGLRKIGAIGAGYKADIVVLNDLDKMEVDS
                     VYKDGNLVNEEMFSNYNYEIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEIVPYQIL
                     TNKVYESLPCADGYFVPNKEYSKLCVVERHRMTGNVAIAPLKGFGIKNGAIATTVAHD
                     SHNIIVAGDNDDDILVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTFTAEEVQE
                     ITDNMLEIARKMGVPEYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
BASE COUNT          681 a          195 c          332 g          578 t
ORIGIN      
        1 tgaagcagga accctgtgac tttagtcatg ggaggttcag tgtagcaata aatatatatc
       61 acctggcttt attgatgcac atatgcatat tgaatctaca atggtaatgc caattgaatt
      121 atcgaaaaaa ttattaaaat caggtacgac aactatcata gccgacccac atgagttggt
      181 caatgtaaaa ggagttagtg caatagattt tttattagaa tcgacaaaag atataccact
      241 taatgtatat attatggtgc cttcatccgt acctgcaaca agttttgaaa ctaatggagt
      301 tggtaaattt agtgcaaaag acatggagag ttatgtaaat aatcctagaa ttttaggttt
      361 aggagaggta atgtgtttta atgatgttat aaattctgaa aatgaaatct tggataaact
      421 agagcttttt aaaaataaag ttgtagatgg acatgcacct aatataaatg gtaaatcact
      481 tcagacatat gtatgtgcag gaatagaaaa tgaccatgaa tgtataactt ttgatgaagt
      541 atatgaaaaa ttgagagcag gattaaaaat tttaataaga gaaggaagcg cagcaaaaaa
      601 tttaaagtct atagtaagtg gtatgctaaa gcataacttg ccaattgaag aatttatgtt
      661 ttgcacagat gataagcact tagatgatat agaaaaacaa ggtcacataa gatggaatat
      721 aaaatgtgcg attgatttag gtatggaacc tgttagggct ataaaagttg caacttataa
      781 ttctgcaaaa gcatatgggt taaggaaaat tggagcaata ggtgcgggtt ataaggcaga
      841 tattgttgta ttgaatgatt tagacaaaat ggaagtggat agtgtatata aagatggtaa
      901 tttggtaaac gaagagatgt tttctaatta caattatgag attaaagata aagaattgct
      961 taatacagtc aagtttaaat atattaataa agaaaaaata cagttaaaag taagtgaaaa
     1021 aaattatgta atggagattg taccttatca aattttgaca aataaggtat atgaatcttt
     1081 accatgtgca gatggatatt ttgttccaaa taaagagtat tcaaaacttt gtgttgttga
     1141 gagacataga atgactggaa atgtagcaat tgctccatta aaaggatttg gcataaaaaa
     1201 tggagcaatt gctactactg tggcccatga ttctcataat attatagttg caggggataa
     1261 tgatgatgat attctagtag ctataaatta cttaaaggaa atacaaggtg gttatgtaat
     1321 tgtatcaaat ggaaaagttc ttgcacacct aagtcttcaa gtagctggat taattagtac
     1381 ttttacagct gaagaagtac aagaaattac tgataatatg ttggagatag caagaaaaat
     1441 gggagttcca gagtatgtag acccttttat tacactttct tttatggcac ttccagttgt
     1501 tccacaaata agacttacag atttaggtct atttgatgtt gaggaattta aatttatata
     1561 gataatataa aagtaataat aaatgaggct ttctcaaaat agggataagt ctctttttat
     1621 tgttgaaatg ataaaaatat atttgatttc atacaaaaaa gagcatctca tgaactaaaa
     1681 attttatttt tagatatcct tattttaatt aatctatcat ttaaccttgt tttaagcata
     1741 gatatcttaa taaaactatt ttcagtctat tttaacatat aaatac
//
LOCUS       DAMJVI010000233         1662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.239, whole genome shotgun sequence.
ACCESSION   DAMJVI010000233 DAMJVI010000000
VERSION     DAMJVI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.239"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..368
                     /locus_tag="PC545_003745"
     CDS             <1..368
                     /locus_tag="PC545_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2458433.1"
                     /translation="TINARYLKTKYNAGVTNRNCDNSGVLVNAVLTPDRVNKRQNGRR
                     IKESGEPMFTLTAQDKHGILKNGDIRRLTPKECFRLQGFPDKYYERAASVCSDSQLYK
                     QAGNAVTANVIYEIAKRMG"
     gene            450..680
                     /locus_tag="PC545_003746"
     CDS             450..680
                     /locus_tag="PC545_003746"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458434.1"
                     /translation="MQKYKIKFEEKVTLEHEVIVEIPEEISINDICHCIEQKCQRIYD
                     IADYIREFNGRQIDFTEDTCGGTEMLVESFRK"
     gene            935..1120
                     /locus_tag="PC545_003747"
     CDS             935..1120
                     /locus_tag="PC545_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458435.1"
                     /translation="MVITEFKVTWQTTREWEKGNVYSSIFTNLEEARAFKKDEENIKE
                     NYNVKLLSRAIVEIEID"
     gene            1134..1373
                     /locus_tag="PC545_003748"
     CDS             1134..1373
                     /locus_tag="PC545_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015981545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458436.1"
                     /translation="MNTLIYKYIVNEHRDEADELDRDYGEFKIFNGGKCFGGCGRNFY
                     TLAKFKKMIVTCPHCGKKYQIKLFKNGKVNMRVIE"
     gene            1502..>1662
                     /locus_tag="PC545_003749"
     CDS             1502..>1662
                     /locus_tag="PC545_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2458437.1"
                     /translation="MKVFLVIDGEPVGKERPRMNSITKRIYTPNKTRDYEELIKWLYQ
                     SKVKYRFTG"
BASE COUNT          696 a          139 c          360 g          467 t
ORIGIN      
        1 taacaataaa tgctagatat cttaaaacaa aatataatgc aggtgtgaca aatagaaatt
       61 gtgataatag tggagtttta gttaatgcag ttttaacgcc cgatagggta aataaaagac
      121 aaaatggtcg tagaattaaa gaaagcggag aaccaatgtt cacattgaca gctcaagata
      181 aacatggaat tttgaaaaat ggagatataa gaaggttaac accaaaggaa tgctttaggt
      241 tgcaaggatt tccggataaa tattacgaaa gagcagcaag tgtatgctca gatagtcaac
      301 tgtacaaaca agcgggaaat gctgttactg caaatgttat atatgaaata gcaaaaagaa
      361 tgggctaaaa gttgcaaaat gtcttttagt ataaattatt gttgaagtgt tttgtaactc
      421 tcaaaaacta aaataaaggg ggaattaaat tgcaaaaata taaaattaaa tttgaggaaa
      481 aagtgacttt agaacatgaa gttatagttg aaataccaga agaaataagt ataaatgaca
      541 tttgccattg tatagaacaa aagtgtcaaa gaatatatga tatagcagat tatataagag
      601 aatttaatgg gagacaaata gattttacag aagatacttg tggagggact gaaatgttag
      661 ttgaatcatt tagaaaatga aagtagtgag catatgacta ataaagatat gtgcaagtca
      721 aagaatcttg atgaaagaga agtgtataag agttttggga aagagatttg taaatgataa
      781 aggagattgt gaaagtaaag attgtgatgc aacatatgaa aattggttgg agaaggatgc
      841 tgaaagataa ttataaaaat aaagtcaagg taagcttgtg aatgaaacta gaagtctata
      901 gacttacttt gacttataaa aaggagtggt caatatggtt attacagaat ttaaggtgac
      961 ttggcagaca actagagaat gggaaaaagg gaacgtgtac agctctatat ttactaattt
     1021 agaagaagca agagctttta aaaaagatga ggaaaatata aaagaaaatt ataatgtgaa
     1081 gcttttaagt agagctatag tcgaaatcga aatagattag gtaggtgttt ttaatgaata
     1141 ctctgatata taaatatata gttaatgaac atagagatga agcagatgag ttagatagag
     1201 actatggaga atttaaaata tttaatggag gtaaatgttt tggtggatgt ggtaggaatt
     1261 tctatacatt agctaagttt aaaaaaatga tagttacttg tccccattgt ggaaaaaaat
     1321 atcaaataaa actttttaaa aatggaaaag tgaatatgag agtaattgag taaatcaagg
     1381 gaagtgatta tatgaaaaga agaagatgca gttggtgtgg caaactattt tatcttgatg
     1441 aaaaatctaa ggagatttat tgttgtaagg aatgtaggaa gaaggctaag aaggtgaaaa
     1501 aatgaaagtt tttcttgtaa tagatggaga gccagttggc aaagaaagac ctagaatgaa
     1561 ctctataact aaaaggatct atacacctaa taaaactaga gattatgagg agttaataaa
     1621 atggctgtat caatctaaag tgaagtatcg ttttactggt ta
//
LOCUS       DAMJVI010000234         1612 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.240, whole genome shotgun sequence.
ACCESSION   DAMJVI010000234 DAMJVI010000000
VERSION     DAMJVI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.240"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            71..544
                     /locus_tag="PC545_003750"
     CDS             71..544
                     /locus_tag="PC545_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2458438.1"
                     /translation="MFKEYLIRELKEDDIKNALDLIWNVFEEFESPDYCDEGITTFHQ
                     FIEYNSIIEKIKEKTLFFWGCFDSKKLIGVLAIRNLNHICMLFVDKNYHKHGVAKKLM
                     TTGIQYCICNKFNLTEITVNSSPYAVEVYHKLGFKDTAVEQIVDGIRFTPMKLLI"
     gene            complement(913..1470)
                     /locus_tag="PC545_003751"
     CDS             complement(913..1470)
                     /locus_tag="PC545_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2458439.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAEKLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNSQMIKAAIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSFL
                     SKEEAIKGLVDSIDTVLLGIHKKYD"
BASE COUNT          590 a          212 c          170 g          640 t
ORIGIN      
        1 aattgtattg aattgaattt atttaagata tataattata attaaaataa taataaaggt
       61 gttgtttata atgttcaaag aatacttaat aagagaatta aaagaagatg atattaaaaa
      121 tgcattagac ttaatctgga acgtatttga agaatttgaa tctccagatt attgtgatga
      181 aggaattacg acatttcatc aatttataga atataactct attattgaaa aaatcaaaga
      241 aaaaactctc ttcttttggg gttgttttga ctctaaaaaa cttattggag tcttagctat
      301 tagaaattta aatcatatat gtatgttatt tgtagataaa aactatcata aacatggtgt
      361 tgcaaaaaaa ttaatgacta caggcataca atactgtatt tgtaataaat ttaatttaac
      421 tgaaataact gtaaattcat caccttatgc tgttgaagtt tatcataaat taggatttaa
      481 agatactgct gttgagcaaa ttgttgacgg tatacgtttt acacctatga aattgttaat
      541 ataataaatt aaataaaaag caaagtctct taaatatttt atagttactt tgcttttaaa
      601 ccaagattta aaataatact atcttttttg ttttttaata atatacttta aaatccttac
      661 taatatgaac tataatttat tttttgatat cctttttgat taacatataa attaattcaa
      721 ttattaaaca taaactatgt tgtttctctt tatatctctt ctttgtaaac aatattgggc
      781 atttgaaaat aaatattctt aaaatatact gttctcctgt tgattttcct ttggtacaaa
      841 tgataaaaat ttaatttaca tgccccataa tgaagtgttc aataaataag ttaaaataat
      901 ttatttacct ccttaatcat attttttatg aatacctaat aacacagtat caatagaatc
      961 gactaaacct tttatagctt cttctttact caaaaatgat ttcccaagtt gcaaagtcga
     1021 ataaccatga agtacactca ttattaagtt taatacttca cctgtattct ttgtatttag
     1081 attacaagaa agaataagtt tttctaataa ggatttatat tcatcaaaga tttctaatgt
     1141 ttcattattt ccatgccaag tagcccactg aattgtttca taaacaccag gattagaaat
     1201 tataaaatta aagtatgcta tacttataca ctttatagct gtatctccaa aatttccaat
     1261 agccgccttt atcatttgac tattcatctc tctcattcct ttatgagcta cttcaagtaa
     1321 taaatcatct agactttcta tatgattgta caaagatggt gtacgtattc ctagcttttc
     1381 tgcaataact tttaaagaaa ctttgttaag cccatcttta ttaactatat ctgatgtagc
     1441 ttgtataact atttctctac taacacgcat gattttccct ctttcattta aaattaatta
     1501 ctttattagt ttaactaatc taattagttt cgtcaattat tgtaatttat aaaattcttt
     1561 atactattat aaaatcttga cagtttaatt ataatttaat ataatactaa tt
//
LOCUS       DAMJVI010000235         1608 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.241, whole genome shotgun sequence.
ACCESSION   DAMJVI010000235 DAMJVI010000000
VERSION     DAMJVI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.241"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            91..1551
                     /locus_tag="PC545_003752"
     CDS             91..1551
                     /locus_tag="PC545_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HDF2458440.1"
                     /translation="MKFYKRILTLTLSISFVFANIQLVDAISSVEKIQGKNKYEIAGK
                     IADKNAYKTAYKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSA
                     RLKNVSKVYIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVN
                     TVMLTNAYKGEADAISIASVAARDKAPIILTNGQSIPFSTSGLKSYVIGGTASMSTTL
                     VNNTKSTRLGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHGPMVLV
                     NDGSNKSILKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGVVKNVKPTTKTIKDGM
                     YKVGKDISAGEYLITSNSGSYASYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYI
                     KIEDSTMTLAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNDSLG
                     NAEGIVTNDTFSGRRYITVEEDQYLILNDCYLIENE"
BASE COUNT          660 a          168 c          275 g          505 t
ORIGIN      
        1 gcaattttac ataagtttgt taagtacata actaatttaa gaatatgtat ttatatatta
       61 aaaatttatt atgaggggga caattttatt atgaaattct ataaaagaat attaacattg
      121 actttaagta tttcttttgt atttgcaaat attcaattag tagatgcaat atcatctgta
      181 gaaaaaatac aaggaaaaaa taagtatgaa atagcaggta aaatagcaga taaaaatgca
      241 tataaaactg catataaaac tgcatataaa actgctatat taattaatac tagtaactct
      301 attgcagatg gattaagtgc aagtggacta gcaggagctt taaatgcccc tatactatta
      361 actgaaaaaa atactatacc aactgagaca tctgcaagat taaaaaatgt atcaaaagtt
      421 tatataattg gaggaacata ttctattagt acatctgtcg aaaattcatt aaaatctaag
      481 aaaatgaaag ttgtaagaat taagggaaat gacagaataa aaacaagtta taatgttgca
      541 aaagaaataa attcaataaa aaaagtcaat actgttatgc taaccaatgc atataaagga
      601 gaagcagatg ctataagtat tgcatctgta gcagcaagag ataaagcacc aataattctt
      661 actaatggac aaagtatacc tttttcaact agtggtttaa agtcttatgt aataggagga
      721 acagcttcaa tgagtaccac tttagtaaac aatactaaat ctactagact aggggggagt
      781 actcgttttg aaactaataa agcaataata aataagtttt ataaggatgc tagagagttt
      841 tatatagctg gagcttatga attaacaaat gcacttgtag gatcttcttt atcaaaacat
      901 ggtccaatgg ttttagttaa cgatggtagc aataaaagca ttttaaaaaa tgctaagaag
      961 ataacttcta taggatatat agattcaaat atagtacaac agtgtttaaa tataacaaat
     1021 ggaattggag atattaatac tggggttgta aaaaatgtta aacctactac aaagactata
     1081 aaagatggaa tgtataaggt tggaaaagat atctcagcag gtgaatactt aataacttca
     1141 aactcaggaa gttatgcttc ttattatgaa gttacaagtg atagtacagg taatgctgat
     1201 agtatacttt caaatgatat ttttagtggg actagatata tcaccttaaa gaatggtcaa
     1261 tatattaaaa ttgaggattc aacaatgaca ttagcaaagt atgctaaagc ccagaaggcc
     1321 aagaatggta agtttggaaa tggtatgtat aaaattggat tagaaattcc tgctggagaa
     1381 tatattataa tgtcaaattc ttctgatgca tattatgaag ttagaaatga tagcttaggt
     1441 aatgcagagg gaatagttac taatgatact ttttcaggaa gaagatatat aacggttgag
     1501 gaagatcaat atttaatatt aaatgattgt tatttaattg aaaatgaata gatgtaattg
     1561 tatttaatag taaatcatat attttattta aggaaaggtg ttttaagt
//
LOCUS       DAMJVI010000236         1409 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.242, whole genome shotgun sequence.
ACCESSION   DAMJVI010000236 DAMJVI010000000
VERSION     DAMJVI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.242"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(597..1352)
                     /locus_tag="PC545_003753"
     CDS             complement(597..1352)
                     /locus_tag="PC545_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HDF2458441.1"
                     /translation="MAHICFEDICYLLLKEAMKLKLIPHNKIVLFEDDLSIGNIFDVN
                     NYELRENIFKIRNVFENNPKLLFKPDLLEDRKLSKCRNIIIWCANNPKDLCGVYYVVS
                     KFQDKNIKIALVDEKKTEDALIRYSFTSEMSPEDFPFFLDKTLEIGFNEKLEFKNKWD
                     VLVKENSIFRALVGNEIKSVEETYFDKYILRNISDKWQPLIEVVANCMFEDEMRVKDW
                     FILWRLEKMASLKIVKIKGSKGDFYSDKEIRKV"
BASE COUNT          500 a          212 c          103 g          594 t
ORIGIN      
        1 actataatta ataaaagagt atattttaaa tctaaaagct ataggttgaa acatactctt
       61 tttattttgt ttatttatag ctttagttat taatcatata taaatctaca aaacgtattc
      121 ctagtaactt atattttttg atatctacaa tcataagact atattattgg attcatacct
      181 caaaaatatt gccttctgaa cattattata ttcaaagcta tgttaattta caatgatata
      241 tcctaacatg tattgattaa aaaaaattaa aacttgcttt tcatctccta tatctaaaga
      301 agtggtattt agaataaact attgtaatat taaagttgaa attttctagt ttatttactc
      361 tatagcacca tattttaata ctattaagta aaaatgtagt taaaatatat actaatatcc
      421 ctgaaataag tatttctttt ttatttagca cacttattcg taaggtatta gttattatat
      481 ttccaaatta tagaataaat attatactta ttgctaatta taatattaat actgttaaga
      541 tatttttctt catagttata cccattattt aatacaattt attataaatt caatttttaa
      601 acttttctta tctccttatc agaataaaaa tcccctttac ttccttttat ttttactatt
      661 tttaaacttg ccatcttctc taatctccaa agtataaacc aatcttttac acgcatttca
      721 tcttcaaaca tacagtttgc aaccacttca attaaaggtt gccatttgtc actgatattt
      781 cttaatatat atttatcaaa ataagtttct tcaacacttt taatttcgtt acctactaat
      841 gctctaaaaa tagaattttc tttaactaac acatcccatt tatttttaaa ttctagtttt
      901 tcattaaagc caatttctag tgtcttatct aaaaagaatg gaaaatcttc tggagacatt
      961 tcactggtaa atgaatatct aattagagca tcttctgtct ttttttcatc tactaatgca
     1021 atctttatat ttttatcttg aaacttactc actacataat atactccaca caaatctttt
     1081 gggttatttg cacaccatat tattatattt ctacattttg ataatttcct atcttctaac
     1141 aaatctggct tgaataatag tttcggatta ttttcaaaaa catttctaat cttaaaaata
     1201 ttttctctta attcgtagtt atttacatca aaaatattac ctattgataa atcatcttca
     1261 aataatacaa ttttattatg tggtattaac tttaacttca ttgcttcttt taataacaaa
     1321 taacaaatat cctcaaagca tatatgtgcc ataaaattca cctcttttaa aattaacaat
     1381 ttattttatt ataatatata aactgtaat
//
LOCUS       DAMJVI010000237         1337 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.243, whole genome shotgun sequence.
ACCESSION   DAMJVI010000237 DAMJVI010000000
VERSION     DAMJVI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.243"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            27..101
                     /locus_tag="PC545_003754"
     tRNA            27..101
                     /locus_tag="PC545_003754"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:59..61,aa:Thr,seq:tgt)
     gene            116..200
                     /locus_tag="PC545_003755"
     tRNA            116..200
                     /locus_tag="PC545_003755"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Tyr,seq:gta)
     gene            210..293
                     /locus_tag="PC545_003756"
     tRNA            210..293
                     /locus_tag="PC545_003756"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:244..246,aa:Leu,seq:tag)
     gene            323..399
                     /locus_tag="PC545_003757"
     tRNA            323..399
                     /locus_tag="PC545_003757"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:357..359,aa:Arg,seq:tct)
     gene            407..482
                     /locus_tag="PC545_003758"
     tRNA            407..482
                     /locus_tag="PC545_003758"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:440..442,aa:Gln,seq:ttg)
     gene            571..659
                     /locus_tag="PC545_003759"
     tRNA            571..659
                     /locus_tag="PC545_003759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:605..607,aa:Ser,seq:tga)
     gene            663..738
                     /locus_tag="PC545_003760"
     tRNA            663..738
                     /locus_tag="PC545_003760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:696..698,aa:Phe,seq:gaa)
     gene            745..818
                     /locus_tag="PC545_003761"
     tRNA            745..818
                     /locus_tag="PC545_003761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:779..781,aa:Met,seq:cat)
     gene            833..909
                     /locus_tag="PC545_003762"
     tRNA            833..909
                     /locus_tag="PC545_003762"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:867..869,aa:Ile,seq:cat)
     gene            933..1009
                     /locus_tag="PC545_003763"
     tRNA            933..1009
                     /locus_tag="PC545_003763"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:967..969,aa:Pro,seq:tgg)
     gene            1017..1093
                     /locus_tag="PC545_003764"
     tRNA            1017..1093
                     /locus_tag="PC545_003764"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1051..1053,aa:His,seq:gtg)
     gene            1102..1177
                     /locus_tag="PC545_003765"
     tRNA            1102..1177
                     /locus_tag="PC545_003765"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1135..1137,aa:Lys,seq:ttt)
     gene            1185..1258
                     /locus_tag="PC545_003766"
     tRNA            1185..1258
                     /locus_tag="PC545_003766"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1217..1219,aa:Cys,seq:gca)
     gene            1265..1336
                     /locus_tag="PC545_003767"
     tRNA            1265..1336
                     /locus_tag="PC545_003767"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1297..1299,aa:Asn,seq:gtt)
BASE COUNT          320 a          309 c          355 g          353 t
ORIGIN      
        1 ccccttccag gtcgccaaaa taaaatgctg gtgtggctca acggtagagc agctgacttg
       61 taatcagcag gttgtaggtt cgattcctat caccagctcc atgtaaaatt aatacggagg
      121 atttcccgag cggccaaagg gggcagactg taaatctgtt gtcatcgact tcggtggttc
      181 gaatccacca tcctccacca aaaaaatgcg cggatgtggc ggaattggca gacgcactag
      241 acttaggatc tagcgccatt ggcgtggggg ttcgactccc ttcatccgca ccatttttgt
      301 ttgatataca tatatatatt gtgggtgcat agctcagctg gatagagcaa cgcccttcta
      361 aggcgtgtgt ccggggttcg aatccctgtg cgctcaccac atatattggg gattagccaa
      421 gtcggtaagg cacatgactt tgactcatgt atgcgtaggt tcgagtcctg catccccagc
      481 caaatgatgt attactaagc tatatttgaa ttatttttat tttaaatata gttaggttca
      541 attttagaat aagtttaagt atccgaatgt ggagaggtgt ccgagtggtt taaggagctg
      601 gtcttgaaaa ccagtgactt cgaaaggggc cgtgggttcg aatcccaccc tctccgccaa
      661 atgcccagat agctcagtcg gtagagcagg ggactgaaaa tccccgtgtc ggtggttcga
      721 ttccgcctct gggcaccaaa atatggcggt atagcttagt tggctagagc gttcggttca
      781 tacccgaaag gtcataggtt cgactcctat taccgctact aaaatttaat gtgggccatt
      841 agctcagttg gttagagcgc ccggctcata accggtaggt ctggggttcg agtccctgat
      901 ggcccaccac attaaattaa ttaatataaa ttcgaggtgt agcgcagttt ggtagcgcac
      961 atggtttggg accatggggc cgggggttcg agtcccttca cctcgaccaa tataaggtgg
     1021 gtatagctca gttggttaga gcgccagatt gtggctctgg aggtcgtgag ttcgactctc
     1081 attatccacc ccaattcata tggtctatta gctcagtcgg tagagcacct gacttttaat
     1141 cagggtgtcc cgcgttcgag tcgcggatag atcaccaaaa aggtggcgac atagccaagt
     1201 ggtaaggcag tggactgcaa ctccttgatc cccagttcga atctgggtgt cgcctccaaa
     1261 atattccaag gtagctcaac ggtggagcaa ccggctgtta accggtaggc tgtgggttcg
     1321 agccccaccc ttggagc
//
LOCUS       DAMJVI010000238         1267 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.244, whole genome shotgun sequence.
ACCESSION   DAMJVI010000238 DAMJVI010000000
VERSION     DAMJVI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.244"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            184..1260
                     /locus_tag="PC545_003768"
     CDS             184..1260
                     /locus_tag="PC545_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDF2458442.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASMLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYEDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          500 a          177 c          221 g          369 t
ORIGIN      
        1 ttcagaaaat tcagacttat gaaaaggtag aaaatgaatg ttttcataaa atatataaaa
       61 taaattatat ataaaaaata aattttatgt tgacatagtt aattcagctt gatattatag
      121 ttattaaaaa taaggacagt aaaaacgcta tccatattac aaactaaaaa ggggggaatt
      181 ataatgagta agaaattatt tattcctggt cctattgatg ttaaagaaga agtcttgcaa
      241 aagatggcta caccaatgat aggacataga ggaaaagatg catctatgct ccaaaagagt
      301 ataagtgaaa agatgcaaaa actattctat acaaacaaca cgatactttt atccacgtca
      361 tccggaactg gtttaatgga aggctccatt cgttcctgta catccaaaaa ggcagcagta
      421 ttttcctgtg gatcctttgg tgatagatgg tacaaaatgg cagtggctaa taatgtccca
      481 gcggatatat ttaaagttga attaggagag gcaacaacgc cagaaatggt tgataaggtg
      541 ctttctaccg gagaatacga tttaataact gtaactcaca atgaaacttc aactggaata
      601 agaaatccaa tagaagaaat tggagaagtt gttaaaaagt atgaagatgt aatctactgt
      661 gtagatactg taagttcagc cggtggaata aaagtcgagg ttgataagat tggaatagat
      721 atttgtataa catcagtaca aaaagcactg ggattaccac caggaatgtc tatttgcaca
      781 ttttctcaaa aagcaatcga tagagctaag caagtacctt ttagaggagt atatcttgat
      841 ttgttagcaa tgtatgaata cttaatcaag aaaaactacc aatacccttc aacaccatct
      901 ctttctcaca tgttcgcttt agatttccaa ttagataata tattagatga aggcctagat
      961 aacagattca acagacatga agacatggca aacttagtta gaaactgggc taaaaaacac
     1021 ttccaaattt ttaccaatga aaatcactta tccaatacac ttactgttat agaaaatact
     1081 caaggtatta gtgtatcaaa tttaaattca aaattacaag aacgaggatt ccaaatagca
     1141 aatggctatg gagatttaaa agaaaaaact tttagaattt cacatatggg tgactatact
     1201 gtcgaagatg tacaagaatt attagataat attgatgata tattaggttt taataaataa
     1261 tattgta
//
LOCUS       DAMJVI010000239         1237 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.245, whole genome shotgun sequence.
ACCESSION   DAMJVI010000239 DAMJVI010000000
VERSION     DAMJVI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.245"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            89..820
                     /locus_tag="PC545_003769"
     CDS             89..820
                     /locus_tag="PC545_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458443.1"
                     /translation="MLSKKNVSLIILVIILSLLTAIFIFNNKNTNNKLKEVTKNNEEI
                     VPKNLEGLSIEQLIEIRGKAIDKNLEGLREFGEDSVKKRKDSILNFEFGLVAFVNKNY
                     SNLTEKEKLIRQINIVNYENSINDIMDPSGSFFELANQNYQVNEFLLKNGKDYKEWSY
                     EGLDFLIDHLEELCKFVDFDVKDVIDIIDPTIKTDLSEEQKKSLNDKLKKISSSDTLS
                     EEVKKEIKNWENNLNNLNMNKNNSN"
     gene            complement(977..1183)
                     /locus_tag="PC545_003770"
     CDS             complement(977..1183)
                     /locus_tag="PC545_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458444.1"
                     /translation="MSKYSSLWEYFKKNNSQSFKLTFEEIKDIAGIEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
BASE COUNT          514 a          133 c          151 g          439 t
ORIGIN      
        1 tctttagaac aaacagtagt aaatgttatc ttcataaaca aatttatttt tcacaagtac
       61 aatacttatt taaaaaagga ggtttaatat gttaagtaaa aaaaatgtaa gtttaattat
      121 tttagtaata attttaagtt tattaactgc tatatttatt ttcaataata aaaatactaa
      181 caataaattg aaagaagtta ctaagaataa tgaagaaata gtacctaaaa acttagaagg
      241 tttaagtata gaacaattaa tagagattag aggtaaagcc attgataaaa atttagaagg
      301 tcttagggaa tttggagaag attcagtaaa aaaacgtaaa gattcaattt taaactttga
      361 atttggatta gtagcttttg ttaacaagaa ttatagcaac ttaacagaaa aagagaaatt
      421 aataagacaa attaatattg ttaactatga aaactctata aatgatataa tggatccttc
      481 aggttcattc tttgaattag caaatcagaa ttaccaagtg aatgagtttc tattaaaaaa
      541 cggaaaagat tataaagaat ggtcatatga aggtctagat tttctaattg atcatctaga
      601 agaactgtgc aaatttgttg attttgatgt aaaagatgta atagatatta ttgatcctac
      661 aataaaaaca gacctttcag aggaacaaaa gaaatctttg aatgataaat taaagaaaat
      721 atcttcaagt gatactttaa gtgaagaagt taaaaaggag ataaaaaact gggaaaacaa
      781 tttaaataat ttaaatatga ataaaaataa ttctaattaa ttttctcata agaaaaatgc
      841 ttatatcgtg ataatgtgaa atgtacataa ttataaacaa aaaaggggct gtgacactaa
      901 aaaatagtgc tacgcccttt tttatttgta ataatcatca atacaaatta ttttattata
      961 tgtaattatt tatgtcttaa ccaattttat cgaaaataac agtcttctct tttaaagaga
     1021 tttttcctac ttgatatcca tattcattta attccttttt atatttcaaa aacgaatggt
     1081 ctatctctat cccagcaata tcttttattt cttcaaaagt taatttaaac gattgactat
     1141 tgtttttctt aaaatattcc cataaagaac tatatttact catcttatat cactcctttt
     1201 aaaatcatat cttatctatt aatttatatt ctatcat
//
LOCUS       DAMJVI010000240         1141 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.246, whole genome shotgun sequence.
ACCESSION   DAMJVI010000240 DAMJVI010000000
VERSION     DAMJVI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.246"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(271..1017)
                     /gene="cdtR"
                     /locus_tag="PC545_003771"
     CDS             complement(271..1017)
                     /gene="cdtR"
                     /locus_tag="PC545_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
                     /protein_id="HDF2458445.1"
                     /translation="MDILIFDNDVCFGMKLKEKINNILIKEGFDNDVVRLYHNANLLL
                     KELTEKNKVRIYFIVVDAKYKISNELCDGLWIAQKIRESDYISPIIFLTNHIEMILGI
                     FDYRLEVMDFILKHDMEIAESKIKACIKIAHKRYIKEKNYRSNFFTIYSDFSLWKISF
                     DEVIYFETSTIPHKIKLVTTSRIFEFYKSLKSLPDLDACFIRVHKSFVVNKYHIVSLD
                     LKKNNIKMSNGHICRISNTYRNILKNIIKT"
BASE COUNT          423 a          139 c          120 g          459 t
ORIGIN      
        1 aaatatgtat ttatatgata tgtcagtaca aaataacaac tgaatatttt aaagagacta
       61 tttcgtactt ttatgattta atgagctatc ttatcgaatc ataatctagg aacaattgtt
      121 ataatatcta atagtagtta tatatgatat agtcagtatg tgttttttca caatatgatt
      181 attatataga ggattcttag ggattactct ttttgtataa aaaagagatg gttcaaattt
      241 ttatcttgag acatctcctt tttctattta ttatgtttta ataatattct ttaaaatatt
      301 tctatatgta ttagatatac gacatatatg gccattactc attttaatat tatttttctt
      361 taggtcgaga gaaacaatat gatatttatt aactacaaaa gatttatgta cacggataaa
      421 gcatgcatct aaatctggta aagactttaa acttttatag aattcaaata ttctagaagt
      481 agtcactaat tttattttat gtggaatagt gcttgtttca aaatatataa cttcatcaaa
      541 tgaaattttc catagtgaga aatctgaata gatagtaaaa aaattagagc gataattttt
      601 ttctttaatg tatcttttgt gagcaatttt aatacaagct tttattttac tttcagcaat
      661 ttccatatca tgttttagta taaagtccat aacttctaac ctataatcga agatccctaa
      721 aatcatttct atatgatttg taagaaatat gattggacta atataatctg attctctaat
      781 tttttgtgct atccataatc catcacataa ttcattactt atcttatatt ttgcgtctac
      841 aactataaaa tatattctta ctttattttt ttcagtaagc tcttttaata acaaatttgc
      901 attatgatat aatcttacaa catcattatc aaaaccttct tttattaaaa tattatttat
      961 tttttctttt agtttcattc caaaacaaac atcattatca aagattaata tatccactaa
     1021 ataccctcct ataaaaaatt caaaagtaaa aaaacaaaag tattttatag aaaatacata
     1081 aaataacact aggagataaa taagattaag gatagttaga attaaggtat atttatgctt
     1141 g
//
LOCUS       DAMJVI010000241         1117 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.247, whole genome shotgun sequence.
ACCESSION   DAMJVI010000241 DAMJVI010000000
VERSION     DAMJVI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.247"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            180..890
                     /gene="rgaR"
                     /locus_tag="PC545_003772"
     CDS             180..890
                     /gene="rgaR"
                     /locus_tag="PC545_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HDF2458446.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNTITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
BASE COUNT          450 a           91 c          187 g          389 t
ORIGIN      
        1 tatctagaaa agtataggaa tttatcgtaa tttaagttta aatattaaag agaatttcta
       61 taagtagttt aatcgacaat ttaaatttat aaagaaattt aaatttgaaa gctagtgagt
      121 acagtttgga aattgaaata aagtaaacta aatgatattt tagatttgga ggtgatatta
      181 tgttcagggt agttatatgt gatgacgaaa aaatacaaag aagcatacta aaagagttta
      241 cagaaaaact tttgtctgaa agatgtctaa attatcaaat actggaattt tcctgtggag
      301 aagatttaat atccaaatac ccagaaaaaa ttgatattat atttttagat atccaaatga
      361 aagatatcaa tggaattgag gtggctaaaa aaataagaaa gtttgatgaa aaagtagaaa
      421 ttatatttac aacggctttt tcagagtatg ccccccaagg ttatgaagtt cgtgcttata
      481 gatatttagt aaagcctata gagtatagta actttgcaat gggtgtaaat ctttgtattg
      541 ataaccttca aagaaagaag gagcgttata tagttttaaa tagtaaaaaa ggatttaata
      601 gaatactgat agattctatt ttatatgttg aaactgtaaa gagagattta gttgtacata
      661 caataggtag agattataaa gctaatatga gcatgaaaca agcagaaaaa ttactgtgtg
      721 aaaatggctt ttttagatgt catacaagtt ttattgttaa tttaagaaga atagaagatc
      781 ttagggataa tacgattact attaatgaac aatttatacc tataagtaag tatagactta
      841 aagatttaaa agtagcatta acaagcttgt tatgtgatat aatgtattaa ttttaaaaag
      901 gtaattgaaa atatcgtaaa aaagatttaa tgtcatcaca gggctattga ttgaaactgt
      961 cttaatatat aaaatgttat ttatattaag acagttttat tatgcaaaat agcagtttat
     1021 agacttttaa tatggtttaa tatgtatatg tagagtttcc attataatag catgattatt
     1081 aattagtagc ttgattatca atatactatt attgatt
//
LOCUS       DAMJVI010000242         1107 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.248, whole genome shotgun sequence.
ACCESSION   DAMJVI010000242 DAMJVI010000000
VERSION     DAMJVI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.248"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            264..944
                     /locus_tag="PC545_003773"
     CDS             264..944
                     /locus_tag="PC545_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458447.1"
                     /translation="MQIDVLIIDGDKNSSQELKNFLEGKGINVYLAYNCKDAIIKIFS
                     KKFDLIFLEIILVDGDGWDFCKKVRSITTCPIIHITYINENQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRRDLFLNNNKNNIKLEDYNRASGVIKLENKMIKLTPTENRLL
                     NYFIENAEKTLTIKEIYEHVWMNEYLDDNYSVVVAVSGLRKKIEKDYKNPQKIITIRE
                     VGYYFNKI"
BASE COUNT          486 a           71 c          177 g          373 t
ORIGIN      
        1 tatgcttatc tttatacagt aaataaaaaa tcaaatgaaa taccaaataa agtgtatatc
       61 ttaaaacaat ttactagaga tacaaggatg ctatgtgaat tttgaagtgt gcagtttaga
      121 taataaatat atataaataa taaagaaaat ggatttatag tatttaaaat ttcttataag
      181 ggtaaaaaat gctattagaa aatgtactaa aaatattttt ccaattcttt atagaaaaat
      241 aatgatattt aggagagatt gtaatgcaaa ttgatgtatt gataattgat ggagataaga
      301 atagttctca agagctgaaa aattttttag aaggaaaggg gataaatgtc tatttagcat
      361 ataattgtaa agatgctatt ataaaaatat ttagcaagaa atttgactta atatttttag
      421 agataatttt agttgatggt gatggttggg atttttgtaa aaaagttaga agtataacta
      481 cttgcccaat tattcatata acttatataa atgaaaatca aagtatttta aatgctttaa
      541 attctggagg agatgattat ttaataaagc ctttaaattt agaaatatta tatgcgaaag
      601 tgaaagctat tttaagaaga agagatttat ttttaaataa taataaaaat aatataaaac
      661 tagaagatta taatagagct agtggagtaa taaagttaga aaataaaatg ataaagttaa
      721 ctccaacaga aaatagatta ttaaattact ttatagagaa tgctgaaaag actctaacta
      781 ttaaggagat atatgaacat gtatggatga atgagtattt agatgataat tatagtgtag
      841 tggttgctgt gagtggatta agaaagaaaa tagagaaaga ttataaaaat cctcaaaaaa
      901 ttataacgat aagagaagta gggtattact ttaacaagat ataaagcttt ataaacagaa
      961 tgaatgataa ggcatagata atatgttagt atattacaag agtttttgtt ctaacgatat
     1021 atttgtaaaa ttagatttaa tttacttaat taaattatag cagtagaata aatataaaaa
     1081 atgtctgaaa tattatagac atcagta
//
LOCUS       DAMJVI010000243         1047 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.249, whole genome shotgun sequence.
ACCESSION   DAMJVI010000243 DAMJVI010000000
VERSION     DAMJVI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.249"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            300..767
                     /locus_tag="PC545_003774"
     CDS             300..767
                     /locus_tag="PC545_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDF2458448.1"
                     /translation="MKFLNVLIVVEDIEKSKKFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKSDSEVKFNGNDAELYFEEDYFDTFVERLSTMRDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSIDEVAKRMDVTVEYIESVLE"
BASE COUNT          395 a           72 c          176 g          404 t
ORIGIN      
        1 tattagtgta tattaatatt ctcttgaaat taaaaaaaca tagatatttc agtaatctaa
       61 aatagtctac aaaaataaca gttttagcaa aattaataag aatacatata gatttaaacc
      121 taaaagtatg aatgtgtctg ttttttacaa gattaaaatt tatattatat gatacaatta
      181 acttaactat ttcaataaga cttataattt ttttctttat actagacctt attgaaacct
      241 ttatagttta attctaatta tatattataa ataaagtatg tgtatattaa ggaggtaata
      301 tgaagttttt aaatgtttta atagtagttg aagatattga gaaatcaaaa aagttttatt
      361 atgatgtttt ggggttaaag gtaatctgtg attttggaga aaatgttgta ttagaaggga
      421 atatatcatt acaagaaaaa aagctttggt tagagtttat taataagagt gacagtgaag
      481 ttaagttcaa tggaaatgat gctgagctgt attttgagga agattatttt gatacttttg
      541 ttgaaagatt gagtacaatg agagatatag attatgttca tctagctata gagcataggt
      601 ggggtcaaag agcaattaga ttttatgatt tagatgggca tattattgag gttggagaaa
      661 ctatgtcatc tgtgtgtaga cgttttttag atagtggtct ttcaatagat gaggtagcta
      721 aaagaatgga tgtaactgtc gaatatattg aatcagtttt agaatagatg gattatatta
      781 aagtaaaact atattttgta tagaagtaag ggctgtaagt aaacttgtat ggaaacaaat
      841 ttacttacag tttttgtttt tgtaagatac agaatataat atttgttata attttatttg
      901 gatttttaaa aagtgcatga ttttccttac atattataat agaatttaat atttattgga
      961 tgatttaaat ttagaaaaat ataagtatta gcttataaaa gataattata cttactatca
     1021 agctgtagaa tcatatatac tatttta
//
LOCUS       DAMJVI010000244         1032 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.250, whole genome shotgun sequence.
ACCESSION   DAMJVI010000244 DAMJVI010000000
VERSION     DAMJVI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.250"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(128..634)
                     /locus_tag="PC545_003775"
     CDS             complement(128..634)
                     /locus_tag="PC545_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HDF2458449.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTIAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
BASE COUNT          353 a          151 c           94 g          434 t
ORIGIN      
        1 atctttttta taatagtatt tatttaaaat taatatttat ttagttagta gcatctactt
       61 agatatcaga taagaaagca cagtcattta cttataataa gttcttttca tatatttttt
      121 attataatta ttcttttaca agaactttta taatttctga aatgtacata gtatgatagt
      181 ctttctctgg ataccatttt ttatcatttt ctttagcatc aaagttttct ggtggcatat
      241 taccatgata tagcttttta catactataa tcatattagc ttcttcaaaa gctaccgtat
      301 catccacaaa atatggtgta agtcctgctt tttctatttt atttatatct ctaccagaaa
      361 tagtgccaca aatatttaaa gcttctctat acgtttcatc aaagaaggca attgtaaatg
      421 tatcattact atctacaaat tcttttgtat atctctgtgg tcttatgtaa gttgtaacaa
      481 catttttacc ccaatagact ccaacaccac cccagctagc agtcattgtg ttaaactttt
      541 ctgaatcacc agctgttata agtaaccatt ctttgccaat cttagtaaat ggattaaact
      601 gtaattcctc aatttttact tctctaaaac ccatgacttc atcctaccct tcatttttta
      661 attatataaa ttaattgtta aaaatttgaa tatatgtact cttataatac tgacttttga
      721 ttgtcctaca taattcatgt aaattataat acaacaaatc aattactttc gcaataagta
      781 gttttcatat tttataagtg taaaatatag ataaaatata ttgattataa agtatataat
      841 atatttttaa acctaaagca atatacaata aaaaaatgta gaaagtaagt atctctctac
      901 attttttatt gtatattatt tttatattaa tttaatttta tcttgattca gttttataaa
      961 aaattgttat aattgtcaag ttctactcta ttatttataa tttcttatat cttattaaca
     1021 acatttaagt ta
//
LOCUS       DAMJVI010000245          951 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.251, whole genome shotgun sequence.
ACCESSION   DAMJVI010000245 DAMJVI010000000
VERSION     DAMJVI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.251"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            146..688
                     /locus_tag="PC545_003776"
     CDS             146..688
                     /locus_tag="PC545_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDF2458450.1"
                     /translation="MKICITVGHSILKGGKSTGVNGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDVIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGT
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
BASE COUNT          403 a           79 c          155 g          314 t
ORIGIN      
        1 atgataaaat aagacaaata tgttaaaaaa atgataatca cggagaactt ttaccttctt
       61 caattaatat attattatta gtaataactt taaaattata ttactattat ataaaaattg
      121 gcttaaaaga aggtgaaaaa agtaaatgaa aatatgcata acagtaggtc atagtatact
      181 aaaaggtggt aaaagtacag gtgtaaatgg tatagttgat gaatacaatt ataataaaaa
      241 attagctcct atgttagctg agatgcttat aagtcaaggt aatacagtag atgtaataat
      301 ttgtccagaa aggcacttta tgtcagaaag agaagaattt ttctatagag ttccaaaagt
      361 aaacagtggt aaatacgatt tattagtaga gctacattta aataaagctg atggaacaca
      421 atgtggtaca gaagtgcttt actatggcac tgaaggatta gaatatgcta aaagagtatc
      481 taatagactt ggagaattat ttgaaaatag aggagctaaa aaaagagaaa atttatatat
      541 attgagaaat acaaatccag ttgcaataca gattgaaagt tttttctgtg ataatgtgaa
      601 tgattgtaac aaagcaaatg aatcaggtta tgattatata gcacgattga ttacagaagg
      661 tatattgaat aaagatataa atatgtgaga ttatggtgat ttaggtattg attaatacct
      721 tttaatatta gatataaata aaaatacaat gtattagtat aatttaaact ttcaaaagat
      781 agattagttt gattattgac aagagatacc tccttgaaaa tattgattta tcagtatatt
      841 taaggaggta tttaaatata aaaatttaat taatttgcta ctaaactata ggtttataga
      901 gtatatattt ttatcttcag tctaataaat aatataaaaa tagtagaacc t
//
LOCUS       DAMJVI010000246          883 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.252, whole genome shotgun sequence.
ACCESSION   DAMJVI010000246 DAMJVI010000000
VERSION     DAMJVI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.252"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            1..346
                     /locus_tag="PC545_003777"
                     /pseudo
     CDS             1..346
                     /locus_tag="PC545_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            656..>883
                     /locus_tag="PC545_003778"
     CDS             656..>883
                     /locus_tag="PC545_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458451.1"
                     /translation="MNFRNIYLILMGLGIIILTTIISLIEIKIGFCNEKYFNKLESKY
                     GNIDRKRTIKLEVLYRYVTGFEYIAIGLFTKR"
BASE COUNT          333 a           88 c          128 g          334 t
ORIGIN      
        1 taagctaatt aaaaacctta agaagaacga tattgacact acttgaatta tattatagaa
       61 aagctgaaat aaataaaata gtttgggcta gaataggttt taatttatat ctttctaaag
      121 gagttgcatt aactttaact gatgctggta tagggatttc aggacttttg gctggtgcta
      181 ttcctggtat aggacagtac ttgccactta tagttagatt aattacaatg taccttgagt
      241 caaatataac atctggaaaa atctttaaat ttaaaaaata cgttataata aaggaggtgc
      301 ttgtgtaacc aaatactact tacaatctgt agaaatcaat aggtgataaa atgattataa
      361 aatatctact ttttgcaatt gtttcagcat taattttatt attttgtaat aaagcattcc
      421 aactaagtaa attacacaag ttcattatat tgtttgtttc attttttata ctaacttttg
      481 tagcatcatt cattagtaga taaaaaataa gatggtgggg ttaacttacc atctttttct
      541 catttaatat ttgtaatatt ttagatactc tttatacttg tgttactgtt atactttgat
      601 taggaaaatt aatacaacat gagttgatga ttatattata aaggaggtat cttctatgaa
      661 ttttaggaat atttatctta tacttatggg attgggaatt ataatattaa ctacaataat
      721 atctttaatt gaaattaaaa ttggtttttg taatgaaaaa tattttaata aattagaatc
      781 aaaatatgga aatatagata gaaaaagaac tattaaacta gaagtacttt atcgatatgt
      841 aacaggtttt gaatatatag caataggatt atttacaaaa aga
//
LOCUS       DAMJVI010000247          826 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.253, whole genome shotgun sequence.
ACCESSION   DAMJVI010000247 DAMJVI010000000
VERSION     DAMJVI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.253"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     regulatory      9..102
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            185..670
                     /locus_tag="PC545_003779"
     CDS             185..670
                     /locus_tag="PC545_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDF2458452.1"
                     /translation="MISELMLKNFRKSSINFKTKIKLNLREKPDINSLKLKSIPEGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNNSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKVITTIPDGFKVEVCYSVGKWARVNINKNEKRYSGYVYNQYIEEHLS
                     L"
BASE COUNT          364 a           68 c          130 g          264 t
ORIGIN      
        1 tgttattatt cagatatagg caatattaga taaatctaat gacgcaaaac tatagggact
       61 aaaatcttta tgtaataaag gtcatgtcag ccagttgcca aaaagataat aattcttttt
      121 gttaaaagta tttttagagt tatatatttt aataacttaa tatttggcaa gaggagggct
      181 taagatgata tctgaattaa tgttaaaaaa ttttcgcaaa tctagcatca attttaagac
      241 taaaattaaa ttgaatttaa gagaaaaacc agacataaat agtttgaaat taaaaagtat
      301 accagaaggt aaaattgtta agttaaagtg tgtagatgga atatgggcag aagttgagag
      361 taattatgac aaaggttggt tactttataa atatttagaa agattaagta atgctaaaaa
      421 caatagtaat gatacaataa aaaatagaaa aaagaattac ataggaaata taagaacaaa
      481 tggattaagt ttagaactta gaaatgatag aacattagaa tctaaagtta taactacaat
      541 ccctgatggt tttaaagttg aagtatgtta tagcgttgga aaatgggcaa gagttaatat
      601 caataaaaat gaaaaacgat attctggata tgtatataat caatacatag aagagcattt
      661 aagtttataa tacgatacta gcaagcttta aatatattaa taaatcgcaa aaaagaatcc
      721 cattaatggg attctttttt gtagaaacat tataacagaa ctaaagagaa taaattaact
      781 aatttatttt tattataaag taattataaa tggtaaattt aattga
//
LOCUS       DAMJVI010000248          825 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.254, whole genome shotgun sequence.
ACCESSION   DAMJVI010000248 DAMJVI010000000
VERSION     DAMJVI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.254"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            389..>825
                     /locus_tag="PC545_003780"
     CDS             389..>825
                     /locus_tag="PC545_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458453.1"
                     /translation="MIAVKKAYKFRIYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNDKETFTYKQCSSDLTNLKKELNWLKEPDKFSLQNALKDLDNAYKKFFKEKAGFPKF
                     KSKKINRFSYKTNFTNGNIMYCGQHIKLPKLGMVKVRDKQVPKG"
BASE COUNT          351 a           93 c          127 g          254 t
ORIGIN      
        1 ctccaaatag gagaaacttc aacgactatt cctcttgagg gaagtacact acaagttatc
       61 ggtagtggaa gtgggtagac cttagcagat aatgccgaag gataagatat agtctgtgct
      121 tatgtgaaag tgtaagaagt tcataagaga actgcatagg tagtagcgaa cttatgtgaa
      181 cgacaacctc aaaaatgata aaagttaaga cttacggttc ttatatatgt tatttaaaat
      241 taaatataaa accttctaaa taattgtttt aaataattac atttgttaac attattatag
      301 gtgattaata aattgttcac cacagcatta tataatatat taaatattaa aatcattaaa
      361 aagtgtatca aaaaacaatt aaagtgaggt gatagcagtg aaaaaagctt ataagtttag
      421 aatatatcct aataaaaaac agcaagagtt aattaacaaa acttttggat gttgtaggtt
      481 tgtatataat aaatatcttg ctaaaagaat agatgtttat aaaaacgata aagaaacttt
      541 tacttataaa cagtgtagtt ctgatttaac caatttaaaa aaagaattaa attggcttaa
      601 agaacctgat aaattttcac tacaaaacgc cttaaaagat ttagataatg catataaaaa
      661 attctttaaa gaaaaggcag gatttcctaa atttaaatca aagaaaatta atagattttc
      721 atataaaact aactttacaa atggaaacat tatgtattgt ggtcaacata taaaactacc
      781 taaacttggt atggttaagg taagggataa acaagtacct aaagg
//
LOCUS       DAMJVI010000249          816 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.255, whole genome shotgun sequence.
ACCESSION   DAMJVI010000249 DAMJVI010000000
VERSION     DAMJVI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.255"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(169..>816)
                     /locus_tag="PC545_003781"
     CDS             complement(169..>816)
                     /locus_tag="PC545_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458454.1"
                     /translation="LNRFNLGLQNGEQQNINEIKFSIGSKLYLEIVNNKSKNGYIEGH
                     DSYSNFIIYDIEDNTFNISNKFMDNLNKEKNANPKEIGLLSKTKLDYMVKNNKLNLIV
                     TDMSNSITNLVYDINSNEIKLEDYEILDLKVDYNEASSGVRKVKLLDDKIYSISGIKN
                     SISGELKGFKGIVDVVGTKPVKFQVFDMKLKKNIYEAELINGDISLQEKLFFVAN"
BASE COUNT          281 a          128 c           62 g          345 t
ORIGIN      
        1 agataatctt tttttcatta tcaaaataaa tctaaacaat gaaaatatat ttgatttcat
       61 ataaaaaaag tgtctcatga gctaaaaagt ttattttgag acactccctt acttatactt
      121 atctgtgata catataaaag tttaaagttc taaaattaat tctaacactt aatttgcaac
      181 aaaaaataac ttttcttgta aacttatatc tccatttatt agctcagctt cataaatatt
      241 ctttttaagt ttcatatcaa atacttgaaa ttttactggc ttagttccga ctacatcaac
      301 aatcccttta aatcctttta attctcctga tatactgttc ttaatacctg aaatagaata
      361 tatcttatca tccagtagct taactttcct aactccagat gatgcttcat tataatctac
      421 ttttaaatca agtatctcat aatcttctaa tttaatctca ttagaattaa tatcataaac
      481 aagattagtt atggaattac tcatatccgt aactataaga ttcaatttat tgttttttac
      541 catataatct agcttagttt tacttaataa gccaatctcc tttggatttg cattcttttc
      601 tttgtttaga ttatccataa atttattact tatattaaaa gtgttatctt ctatatcata
      661 tataataaag ttagagtaac tatcatgtcc ttcaatatat ccatttttac ttttattatt
      721 tactatttct aagtataatt tacttccaat agaaaattta atttcattga tattttgttg
      781 ttctccattc tgtaatccta aattaaatct atttaa
//
LOCUS       DAMJVI010000250          806 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.256, whole genome shotgun sequence.
ACCESSION   DAMJVI010000250 DAMJVI010000000
VERSION     DAMJVI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.256"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            553..>806
                     /locus_tag="PC545_003782"
     CDS             553..>806
                     /locus_tag="PC545_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458455.1"
                     /translation="MSGIISVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGAS
                     IVAFSLTNGISPVVATLMAILCGCTAGLVTGILHIKFKIS"
BASE COUNT          268 a           93 c          112 g          333 t
ORIGIN      
        1 cttttatgac ttatttatat ttttcatttt tataaaaaaa caataattaa gaatataact
       61 atattctaca tgtaaaaatt tttttattta aactcaattt tctgataatt tgataatttt
      121 tcaaaattta tagttgactt ttatttttta ctgtattaaa ctaattacta tacaaaacag
      181 aaaacaaaat tttcaaaatt attaaattaa aaggagaggt aaatatgaag actttattaa
      241 acaaacaagt acgatttttt agtagccaat ttagttaggg tttgtaccta agttaagaca
      301 aattgtcata acatagatac aagcccctta ggattatgat ttttaaggat tgtatctatg
      361 ttggctatag taattgttgt taaagttttt attttatcat cataatatag agccacatgg
      421 attatactgt gtggcttatt cgaatttttg ccatacagtt atatatactg tgtggttttt
      481 ttatatcaaa atcatttttt aaaaccaaat tttaaaaacc atttaaaata ttttcaaagg
      541 ggtgttttat ttatgtcagg tattatatct gtaatgacgc aaagtttaat tttatcgatt
      601 atggcactgg gggtttatat aacttacaaa atcttagatt ttccagatat gtctgctgat
      661 ggtagctata caatgggtgc ttctattgta gcattttctc ttactaatgg tatttctcct
      721 gtagtagcaa ctttaatggc tatcttatgt ggttgtactg ctggtcttgt tacagggatt
      781 cttcacatta agtttaagat atctaa
//
LOCUS       DAMJVI010000251          790 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.257, whole genome shotgun sequence.
ACCESSION   DAMJVI010000251 DAMJVI010000000
VERSION     DAMJVI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.257"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            73..771
                     /locus_tag="PC545_003783"
     CDS             73..771
                     /locus_tag="PC545_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDF2458456.1"
                     /translation="MEKKYFEIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPHECQVLSVDLPEHGERKREKNSFNPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPITQWNIPTKILYGKHDELTEIGVVEKFVEKFNCNLTIVENGKHWFNTPQ
                     ELNSLYEWLNTLVK"
BASE COUNT          299 a          106 c          127 g          258 t
ORIGIN      
        1 tatattacaa cattatttta tagtttgatt aatttaataa tcactaatat atcaaactga
       61 aaggagattt ttatggaaaa aaaatatttc gaaattcatg gagttccttc aatcctttgg
      121 ggaaatcctt caagtaaatt atatatttac atacatggac aatgtggcaa taaagaggag
      181 gctgaagctt ttgctaatat tgcagttcca catgagtgtc aagttttaag tgttgatttg
      241 cctgaacatg gagaacgtaa aagagaaaaa aattcattta acccatggca tgttgttcca
      301 gaattagtat ctattatgga atatgcaaag tgccactgga atcaaatttc gttatatgca
      361 aatagcattg gagcatggtt tagtatgtta agctttgaaa aagagaactt aagagagtgt
      421 atttttgtat ctccaattct agatatgcaa cagctcattt caaatatgat gctctgggca
      481 aatgtatcag aagaacagct taaacatgaa ctaattatcc caacttcatt tgggcatact
      541 ctttcatggg aatatctaca atatgtaaaa caacatccta tcactcaatg gaatatacca
      601 acaaaaatat tatatggtaa acatgatgaa cttactgaaa tcggtgttgt agagaaattt
      661 gtagaaaagt ttaattgcaa ccttactatt gtagaaaatg gcaaacattg gtttaacaca
      721 ccacaagaat taaattcatt atatgaatgg ttaaatactt tagttaaata aaaaataatt
      781 atattattaa
//
LOCUS       DAMJVI010000252          787 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.258, whole genome shotgun sequence.
ACCESSION   DAMJVI010000252 DAMJVI010000000
VERSION     DAMJVI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.258"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>787)
                     /locus_tag="PC545_003784"
     rRNA            complement(<1..>787)
                     /locus_tag="PC545_003784"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          238 c          182 g          195 t
ORIGIN      
        1 cgtccatccc ggtcctctcg tactaaggac aggtcttctc aaatttccta cgcctgcgac
       61 ggatagggac cgaactgtct cacgacgttc tgaacccagc tcgcgtacca ctttaatggg
      121 cgaacagccc aacccttggg acctactaca gccccaggat gtgatgagcc gacatcgagg
      181 tgccaaacct ccccgtcgat gtggactctt gggggagata agcctgttat ccccagggta
      241 gcttttatcc gttgagcgat ggcccttcca cgcagaacca ccggatcact aagtccgatt
      301 ttcatccttg ctcgacctgt atgtcttgca atcaagctct cttttgcctt tacactctac
      361 gtacgatttc cgaccgtact gagagaacct ttgagcgcct ccgttacctt ttgggaggcg
      421 accgccccag tcaaactgcc cacctgacag tgtcccaaga ccagattcat ggcctatggt
      481 tagagtccca gtactacaag ggtggtatcc caaggatggc tccgccaaaa ctggcgtcct
      541 ggtttcaaag cctcccacct atcctgtaca tgtagtacca agacccaatg tcaagctaca
      601 gtaaagctcc atggggtctt tccgtcctgt cgcaggtatc cggcatcttc accggaatta
      661 caatttcacc gagtctgttg ttgagacagt gcccaaatcg ttacgccttt cgtgcgggtc
      721 ggaacttacc cgacaaggaa tttcgctacc ttaggaccgt tatagttacg gccgccgttt
      781 actgggg
//
LOCUS       DAMJVI010000253          784 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.259, whole genome shotgun sequence.
ACCESSION   DAMJVI010000253 DAMJVI010000000
VERSION     DAMJVI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.259"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(55..768)
                     /locus_tag="PC545_003785"
     CDS             complement(55..768)
                     /locus_tag="PC545_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458457.1"
                     /translation="MINIIICEGKKSERVILNDYINTFFYNYNFEYNIKEFENGGDLL
                     KKYPNNVDIIFMDIDLNDENGFNVSKEIKKFDESVRIIFVSSSNKYIKKGYEIGVYRY
                     LEKPLKYSLFKEAISSYLKECYKNEAILSIKCKCKIYFIKVDDILYLEVLKNDLTIHT
                     EKGIYTFKSSMKEIESNLEEHNFFRCHKSYLINLMKVNMLDIENNIVFINQNSIIVSR
                     YKIKLLREKLNSCLVDVII"
BASE COUNT          277 a          112 c           55 g          340 t
ORIGIN      
        1 tattttacaa tagatatatt ctattagaat agaatataaa ggcaaaaatt tacctcaaat
       61 tattacatct accaaacaac tatttaactt ttctctcaat aattttatct tataacgact
      121 tacaattatt gaattttgat ttataaatac aatattattt tctatatcta gcatatttac
      181 cttcattaaa tttataaggt agcttttatg acatctaaaa aaattatgtt cttctagatt
      241 tgattctatt tctttcatac tactcttaaa agtatatatt cctttttcag tatgtatagt
      301 taaatcattt tttaaaactt ctaaatataa aatatcatct acctttatga aatatatctt
      361 gcatttacat tttatagata aaatagcttc atttttgtag cattctttta aatatgatga
      421 tattgcttct ttaaatagag agtattttaa gggcttctct aaatatctat aaactcctat
      481 ctcataacct ttctttatgt atttatttga tgaggataca aatataattc ttacactttc
      541 atcaaatttt ttgatttctt ttgaaacatt aaatccattt tcatcattta agtctatatc
      601 cataaatatg atatcaacat tattaggata cttttttaat aaatcgcctc cattttcaaa
      661 ctctttgata ttgtattcaa aattgtaatt ataaaaaaag gtatttatat aatcatttag
      721 aattaccctc tctgattttt taccttcgca aataataata ttaatcatat tacacccctt
      781 atat
//
LOCUS       DAMJVI010000254          724 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.260, whole genome shotgun sequence.
ACCESSION   DAMJVI010000254 DAMJVI010000000
VERSION     DAMJVI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.260"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            214..>724
                     /locus_tag="PC545_003786"
     CDS             214..>724
                     /locus_tag="PC545_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDF2458458.1"
                     /translation="MYNVIDLFAGAGGLSLGFEMTKKFNMVAFVEKNDNAAKTYLENH
                     PSVKRYCDIKRLDFQDILNSVDKIDVVIGGPPCQGFSNANRQKRKIINGNNELVKLYV
                     DAIDKLKPNVFVMENVKTISSNKHSFYLTKKDKNHIINNLKLNIYNKDSVLYDRTEYI
                     NELFNIISIE"
BASE COUNT          318 a           50 c          114 g          242 t
ORIGIN      
        1 aaaagattga taaataaata aaaaatatat tatgttattt atttgaatag attatccaga
       61 agacataaat aagaaaaata atgtgtaagt aaaaatatta gaatttatta aaagctatta
      121 caagtataag aaatttgaaa tgattattca aaaattgaca tgtgaaaaaa aatatgatat
      181 gctgcaataa aagttaatgt gaggtatgtc agaatgtata atgttataga tctttttgca
      241 ggtgcaggtg gtttgagctt aggatttgaa atgactaaaa aatttaatat ggtggctttt
      301 gtagaaaaaa atgataacgc agcaaaaaca taccttgaaa atcatccgag tgttaagcgt
      361 tattgtgata ttaaaagatt agattttcaa gatatattaa acagtgtcga taagatagat
      421 gttgttatag gaggaccgcc atgtcaaggt ttttctaatg ctaatagaca aaaaagaaaa
      481 attataaatg gaaataatga attagttaag ctatatgttg atgctattga taaattaaag
      541 ccaaatgtat ttgtaatgga aaatgtcaaa actatatctt caaataagca ttcattttat
      601 ttgactaaaa aagataaaaa tcatataata aataatttaa aattaaatat ttataataaa
      661 gatagtgttt tatatgatag aactgagtat ataaatgaat tatttaatat tattagtata
      721 gaag
//
LOCUS       DAMJVI010000255          685 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.261, whole genome shotgun sequence.
ACCESSION   DAMJVI010000255 DAMJVI010000000
VERSION     DAMJVI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.261"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            95..>685
                     /locus_tag="PC545_003787"
     CDS             95..>685
                     /locus_tag="PC545_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HDF2458459.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKG"
BASE COUNT          279 a           71 c          149 g          186 t
ORIGIN      
        1 aatcaataga ttaagtgaaa tgctaataaa ttaaaaaagt gaaatatcaa aataaaacgt
       61 caacaaacta aaaattatat gggggaatat caaaatgtta aatgaaacaa gaaaaaaaca
      121 attaactgaa gtatgccaag acttaataag aaatgctagt tattcaggtc aagaagaaaa
      181 cgtagtaaaa gctatagaag aaaattttgc tttactaggt tttgatagtt ggtcaagaga
      241 tagatatgga aatatagtag gatgtataaa aggaaataaa ccaggtaaaa aaatattatt
      301 tgacgggcat atagatacag ttccagttcc agatgcaagt aaatggagtg ttcctccttt
      361 tgaagcgaga atagtagatg ggaaaatata tggtagagga acttctgata tgaaggggca
      421 agtaagtgct atgatggctg ctgtttcata ctttgcagaa gatactaaca aagactttga
      481 aggtgaattg tatgtagcag gtgttgttca tgaagaaata tttgaaggtg tttcagctag
      541 agagataagt aaggctgttc aacctgatta tgttgtaata ggtgaatctt cagaattaaa
      601 cttaaagata ggtcaaagag gtagaggaga aatagtagta gaaacttttg gtaaaccagc
      661 tcactctgct aatccagaaa aaggt
//
LOCUS       DAMJVI010000256          668 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.262, whole genome shotgun sequence.
ACCESSION   DAMJVI010000256 DAMJVI010000000
VERSION     DAMJVI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.262"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..372
                     /locus_tag="PC545_003788"
     CDS             <1..372
                     /locus_tag="PC545_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2458460.1"
                     /translation="DFLQKLSTKLIKENDIICIEDLQVKNMIKNHKLSRLISDVSWSE
                     FIRQLEYKANWYGRQIVKVGKFFASSQICNKCGYKNEEVKDLNIREWICPSCNETHDR
                     DINASINILKEGLRLITIQNK"
BASE COUNT          278 a           75 c          102 g          213 t
ORIGIN      
        1 gattttctac aaaaactatc tactaaatta ataaaagaga atgatattat ttgcatagaa
       61 gatttacaag taaagaatat gattaaaaat cataaactat ctcgcttaat ttcagatgta
      121 tcatggtcag aatttattcg tcaattagaa tacaaggcta attggtatgg tagacaaatt
      181 gtaaaggtag gtaaattctt tgcaagttca caaatatgca ataaatgtgg gtataaaaac
      241 gaggaagtga aggacttaaa tataagagaa tggatttgtc ctagttgtaa tgaaactcac
      301 gatagagata taaatgcaag tataaatata ttaaaagaag gattaagact aataacaatt
      361 caaaataaat aacttatgta agaaccgtag gaactacggg gattgcctgt ggagaattag
      421 taagacatat tttagtatgc aaaattctat gaagcaggaa ccctgtgact ttagtcatgg
      481 gaggttcagt ttagcttaaa acttacttat ctatttcagt ttctattaga attattatta
      541 gactcattaa aataaaagca ctaatataac taataatata tgttaaattc attaactcta
      601 cataaagttt tacaatctaa atgaaataca ataatctgca taaaattcaa tagcttatat
      661 aaaattta
//
LOCUS       DAMJVI010000257          662 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.263, whole genome shotgun sequence.
ACCESSION   DAMJVI010000257 DAMJVI010000000
VERSION     DAMJVI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.263"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            53..>662
                     /locus_tag="PC545_003789"
     CDS             53..>662
                     /locus_tag="PC545_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2458461.1"
                     /translation="MLDAFITIVSSIIEIWSCKKVFDYTSQSKTDLKKLNMYFLFSVF
                     LIISSLYINIGANSRILICIFVMFVFYKSNYKVNIYKCLVISFFYWLILIGINGLSML
                     LVATVSNLDNMNVLLSGSLYRYEAIFLGKIMLGILLYVYKASFKNFKISKKEIYICIP
                     MIANLLSFFVLYDYIINLVKISPERRTAFLVIVLLLILSNISI"
BASE COUNT          233 a           57 c           85 g          287 t
ORIGIN      
        1 ttatgatata ttttataaaa aatataattt aatcattttt gaggatatta ttatgttaga
       61 tgcatttata acaattgttt cttctattat agaaatatgg tcatgtaaaa aagtttttga
      121 ttatactagt cagtcaaaga ccgacttaaa aaaactaaat atgtattttc tcttttctgt
      181 ttttctcatt atttcttctt tatatataaa tataggagct aatagtagaa tcttgatatg
      241 tatatttgta atgttcgttt tctataagtc taactataag gttaatatat ataaatgtct
      301 ggtaatctca tttttttatt ggttaatatt aataggtata aatggattaa gtatgttatt
      361 agtagcaaca gtaagcaatt tggataatat gaatgtttta cttagtggca gtttatatag
      421 atatgaagca atattcttgg gaaagataat gttaggtata ttactttatg tttataaggc
      481 ttcttttaaa aattttaaaa taagtaaaaa agagatatac atatgtattc ctatgatagc
      541 aaatttattg tcattttttg tactttatga ttatattatt aatcttgtaa aaatctctcc
      601 tgaaagaaga actgcttttt tagtaatagt tttactatta atattatcta acatttcaat
      661 ct
//
LOCUS       DAMJVI010000258          645 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.264, whole genome shotgun sequence.
ACCESSION   DAMJVI010000258 DAMJVI010000000
VERSION     DAMJVI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.264"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(144..623)
                     /locus_tag="PC545_003790"
     CDS             complement(144..623)
                     /locus_tag="PC545_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDF2458462.1"
                     /translation="MSNKLIKNLLSGILQILFFLLGLVIVVGGFKSFMYLCFSGEATL
                     QGTIYGILMFILGVSYFIIIKSLIEVLGSSEYSLFVKENVKRFRIIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVFVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          255 a          105 c           50 g          235 t
ORIGIN      
        1 taaaacataa ttatatttta gtattataat cttttaattt tagcattata gtttatgtat
       61 tataccctaa tatattttat tttaaattac tagttagtca ctcttttcgg gttatttatc
      121 tcctatatat taaagtgtca cacctaaata gttaaatcat tatcctcttt tatctttatt
      181 gcctttacaa atccatctgc aattacaaaa ctcatcagag aagttataaa atatacaaat
      241 actggaacag taaaataaaa tccaaatcct aaatctagaa atctcattcc ttttccagta
      301 gtaccaaaag ttgaaataaa ctcaattaga gagtttaaaa gcaaaagata tccaataatc
      361 ctaaatcttt ttacattctc ttttacaaat aggctatatt cagaactacc taatacttct
      421 atcaatgatt ttataatgat aaaatatgat acacctaaaa taaacattaa tattccataa
      481 atagtacctt gtagagttgc ttctccacta aaacagaggt acataaaact tttaaatcct
      541 cctacaacaa ttactaatcc taaaagaaaa aataaaattt gtaatatacc acttagtaaa
      601 ttttttataa gtttattaga catcatgcca actcctttaa aatat
//
LOCUS       DAMJVI010000259          636 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.265, whole genome shotgun sequence.
ACCESSION   DAMJVI010000259 DAMJVI010000000
VERSION     DAMJVI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.265"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..634)
                     /locus_tag="PC545_003791"
     rRNA            complement(<1..634)
                     /locus_tag="PC545_003791"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          140 a          175 c          117 g          204 t
ORIGIN      
        1 ctacttaacg taactcgttg gcccgttcta caaaaagtac gcggtcacac aagaaatgtg
       61 cttccacagc ttgtaagtgc agggtttcag gttctatttc actcccctcc cggggttctt
      121 ttcacctttc cctcacggta ctatacgcta tcggtcacta ggtagtattt agccttggag
      181 ggtggtccct cctgcttccc acagggtttc acgtgtcccg tggtactctg gatcatatct
      241 aaagtcttct cgtttaacat acaggactat taccttctgt ggtgggtctt tccaaacctc
      301 ttcaattacg atacctcttc gttatgatat gtccgcaacc ccaataaaga aaactttatt
      361 ggtttgggct aatccgcgtt cgctcgccgc tacttacgga atcgaatttc tttctcttcc
      421 ttcaggtact tagatgtttc agttccctga gttcccctca ttaagctatg tattcactta
      481 atgatactta gacattactc taagtgagtt tcctcattcg gaaatcttcg gatcaaagtt
      541 tacgtgcaac tccccgaagc ttatcgcagc ttatcacgtc cttcatcggc tcctagtgcc
      601 aaggcatccg ccctgcaccc ttaataactt gaccag
//
LOCUS       DAMJVI010000260          620 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.266, whole genome shotgun sequence.
ACCESSION   DAMJVI010000260 DAMJVI010000000
VERSION     DAMJVI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.266"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            200..>620
                     /locus_tag="PC545_003792"
     CDS             200..>620
                     /locus_tag="PC545_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDF2458463.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLILIS"
BASE COUNT          248 a           49 c           73 g          250 t
ORIGIN      
        1 aatagtaaac tgattgcata aaaatgattt ttatataaat tttaaacaga agaataaatt
       61 tctaacgaaa ttggatattt cttctgtaaa tacatattca aaaaaagaat tataagagtt
      121 tagtcagcag tcgtgtaagt aataaggact ttataaatat atagaaatat aatgatatga
      181 gtataagtag aaaaatttta tgatttttac aattattttt tataaaaaag ggaggttatg
      241 tttgttgaat ttacaatcaa aatgggtaaa ctttttaaag ccaacatatg tcagtgatga
      301 aataaaaaga aaaaaactac aagttataat atttttttct ataacttatg gtttatctta
      361 ttcactaggt ttattagcaa tatgttttaa tcatttaata gattcagaaa ttttagctca
      421 atttatgatg atattgccat tatcttcagt ttctattgca aaattttaca cagagggaag
      481 aaataatgat aaatataatt tctattcatc agtaatatta ttttttatta tttacttttt
      541 tatatttatt attgaatcat taaagttaat aagtattaat caatttcaat taatgtatct
      601 tatgttaatt ttaataagta
//
LOCUS       DAMJVI010000261          612 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.267, whole genome shotgun sequence.
ACCESSION   DAMJVI010000261 DAMJVI010000000
VERSION     DAMJVI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.267"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>612)
                     /gene="dcm"
                     /locus_tag="PC545_003793"
     CDS             complement(<1..>612)
                     /gene="dcm"
                     /locus_tag="PC545_003793"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDF2458464.1"
                     /translation="CLGHCEYDKFANLSYNAMHKPKEDEWFERDIREIRTENIPRADV
                     WCFGFPCQDISVAGKQFGFRGERSSLFFTVTKLIRELKEEDRPKYLLIENVKNLLSVN
                     GGFDFLKVLVELDEIGYDAEWQVLNSKNFGVPQNRERIFIVGHFRGRSTRKVFPIERK
                     SGKNLEQLNNPTHSTNRIYDAVGIARCIRSQAGGGGAKTGLYFI"
BASE COUNT          175 a          130 c           82 g          225 t
ORIGIN      
        1 tataaagtat agacctgttt tagcacctcc acctcctgcc tgacttctaa tacatctagc
       61 aattccaact gcatcataaa ttctatttgt actatgagtt ggattattta gttgctcaag
      121 attttttcca ctttttcttt cgataggaaa tacttttcgt gtacttcgtc ctctaaaatg
      181 tccaacaatg aatattcgtt ctctattttg gggtactccg aagtttttag aattaagaac
      241 ttgccactct gcatcatagc cgatttcatc cagttcaacg agaactttga ggaaatcaaa
      301 tcctccatta acactaagta gatttttaac gttttcaata agtaaatact tgggtctatc
      361 ttcttctttg agttctctaa taagttttgt aactgtaaaa aataaacttg aacgttctcc
      421 tctgaatcca aattgtttcc ctgcaacaga aatgtcttga catgggaatc caaaacacca
      481 gacatctgct cttgggatat tttctgttct aatttctcta atatctcttt caaaccattc
      541 atcctccttc ggtttgtgca tggcattata acttaaattt gcgaatttat catattcgca
      601 atgtcccaaa ca
//
LOCUS       DAMJVI010000262          611 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.268, whole genome shotgun sequence.
ACCESSION   DAMJVI010000262 DAMJVI010000000
VERSION     DAMJVI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.268"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>611
                     /locus_tag="PC545_003794"
     rRNA            <1..>611
                     /locus_tag="PC545_003794"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          172 a          126 c          179 g          134 t
ORIGIN      
        1 tttaactgag cgttcagtta cttgacgtag acccgaaacc gggcgaccta cccatgagca
       61 ggatgaagcg aaagtaaaat ttcgtggagg tccgaaccca cgagcgttga aaagctcggg
      121 gatgacttgt gggtagcggt gaaattccaa tcgagcccgg agatagctgg ttctccccga
      181 aatagcttta gggctagcct caaggtgaga gatacggagg tagagcactg aatgtcctag
      241 ggggtattgc acctaccgaa gactatcaaa ctccgaatgc cgtcatctta tacttgggag
      301 tcagactgtg ggtgataaga ttcatagtcg aaagggcaac agcccagatc gtcagctaag
      361 gtccctaaat gtaagttaag tggtaaagga tgtgggattg cacagacaac caggatgttg
      421 gcttagaagc agccactcat tcaaagagtg cgtaatagct cactggtcga gtgatcctgc
      481 gccgaagatt tccggggcta aaacttacta ccgaagctac ggcatcagta atgatgggta
      541 ggggagcttc ccatacgggt tgaagcatga ccgtaaggac atgtggacag tatgggagtg
      601 agaatgttgg c
//
LOCUS       DAMJVI010000263          577 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.269, whole genome shotgun sequence.
ACCESSION   DAMJVI010000263 DAMJVI010000000
VERSION     DAMJVI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.269"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>577
                     /locus_tag="PC545_003795"
     rRNA            <1..>577
                     /locus_tag="PC545_003795"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAMJVI010000264          559 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.270, whole genome shotgun sequence.
ACCESSION   DAMJVI010000264 DAMJVI010000000
VERSION     DAMJVI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.270"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            163..270
                     /locus_tag="PC545_003796"
     CDS             163..270
                     /locus_tag="PC545_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDF2458465.1"
                     /translation="MGRHLWNPSYFVATVSENTEEQIRKYINNQKKQVK"
     gene            272..>559
                     /locus_tag="PC545_003797"
     CDS             272..>559
                     /locus_tag="PC545_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458466.1"
                     /translation="MVVVEKAYKFRMYPNKKQQELINKTFGCCRFVYNKYLAKRIDVY
                     KNNKETFTYKQCSSDLTNLKKELKWLKEPDKFSLQNALKDLDNAYKKFFKEK"
BASE COUNT          242 a           51 c           88 g          178 t
ORIGIN      
        1 ttacggttct tatatatgtt atttaaaaat aaatataaaa aaacttcaaa atacttgttt
       61 taagtaattg tatattttaa tattatgata ggttatgaaa aatggaagtt actcatggta
      121 gaggatatgc atatagtatg tcaaatatag acagccagta ttatggggag acatctttgg
      181 aatcctagtt atttcgttgc tactgtatca gaaaatacag aggagcagat aagaaaatat
      241 attaataatc agaaaaaaca agtaaagtga ggtggtagtg gtggaaaaag cttataagtt
      301 tagaatgtat ccaaataaaa aacaacaaga gttaattaac aaaacttttg gatgttgtag
      361 atttgtatat aataagtatc ttgctaaaag aatagatgtt tataaaaaca ataaagaaac
      421 ttttacttat aaacagtgta gttctgattt aaccaattta aaaaaagaat taaaatggct
      481 taaggaacct gacaaatttt cattacaaaa cgccttaaaa gatttagata atgcatataa
      541 aaaattcttt aaagaaaaa
//
LOCUS       DAMJVI010000265          548 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.271, whole genome shotgun sequence.
ACCESSION   DAMJVI010000265 DAMJVI010000000
VERSION     DAMJVI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.271"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          220 a           44 c           67 g          217 t
ORIGIN      
        1 cactaaaaat aagaatgtat aaggtggtaa atttatataa aaaaggatat gagttgtaaa
       61 atattaaata atattgtact aattggaata attttaactt tattatagat tattttaata
      121 ctattagtac tgatggtgta ttttaaaatt ggtataagca ttacaaatac gaatatgcca
      181 actgtaatat aagtagctat atacatatgt gctgtcccat atattgtatc attgtttata
      241 ttaaaaagat tatgtaaatt ggtatcaatc aataaatcat tttcagaaga aatacctaaa
      301 aacttaaaaa atacctatat gtgtatttag ttagatttga gatttttaca tttaatttta
      361 tagtgacaat attatattat ttttatgatg tctatttata tgcaattact ataatatcag
      421 aacttatagt atcatttgtt tcattatcaa taaggttttt tactttagtt ttgtcaattt
      481 tattaaaaaa agttattgat ataaaagata agaaggataa aactatatga cggatttcaa
      541 acataaga
//
LOCUS       DAMJVI010000266          538 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.272, whole genome shotgun sequence.
ACCESSION   DAMJVI010000266 DAMJVI010000000
VERSION     DAMJVI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.272"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(161..460)
                     /locus_tag="PC545_003798"
     CDS             complement(161..460)
                     /locus_tag="PC545_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458467.1"
                     /translation="MKWIKWYSITCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKKNPVDGTTTIYYSYDNSTHIVRLSHPEDYSRKINWDKVSNIRFD"
BASE COUNT          202 a           71 c           54 g          211 t
ORIGIN      
        1 ttttcaatat attttataaa ctaatatatt ttacaaattg ttgagtaata ttttatgata
       61 taaatcagaa gaggcacttc tataatagaa agtgctcaac tatgaataag attaataata
      121 tatttgcctt agatatatag acttttataa atattaaaga ttaatcaaat ctgatgtttg
      181 aaactttatc ccaattaatc ttccttgaat agtcttctgg atgtgataat ctaactatat
      241 gagttgaatt atcatagctg taataaattg tagtagtacc atcaactgga ttctttttgt
      301 atccttgata cacagcacta tttggtactt tcttttcaac aaagctatat gcagatgaag
      361 tatcaatagt ttctatttta ttaggaaata taaacatata aaaagctact actataaaaa
      421 tacatatgca tgtaatacta taccatttaa tccatttcat aaaattcctc ctactttacc
      481 aaaatatact attattattt ccatatattt ataatattta tgcatacgat aaaatttt
//
LOCUS       DAMJVI010000267          507 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.273, whole genome shotgun sequence.
ACCESSION   DAMJVI010000267 DAMJVI010000000
VERSION     DAMJVI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.273"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            53..481
                     /locus_tag="PC545_003799"
     CDS             53..481
                     /locus_tag="PC545_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458468.1"
                     /translation="MKIHSIQNLTLDTRNLIKKYSLTLNEDLIWEFKHGKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESSFSKYEPYKYSYQNEFVKCEMYDMNFMFH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
BASE COUNT          213 a           48 c           65 g          181 t
ORIGIN      
        1 aataaaacac tataatttaa tacttattaa atatttatga ggagaataaa ttatgaaaat
       61 acattcaata caaaatttaa cactagatac gcgtaacctt ataaaaaaat attctcttac
      121 tttaaatgag gatttaattt gggagtttaa acatggtaaa taccacacga taaaatactt
      181 tacacataag tttgcagtta aggagtctgt tcttgcactt ttgttcaaca tacacaaatt
      241 atgttatgct aaaataaaat attttgagtc gagtttttca aaatacgaac cttataaata
      301 tagttatcaa aatgaatttg ttaaatgtga aatgtatgat atgaatttta tgtttcataa
      361 atatagtaat ataatgatag atttgagaaa tttacaagaa attaaaacca tagaagaatt
      421 tagattattt tgtgattacc tagaaagctt tgaagataat ttacaggatt taattatata
      481 gtattttagg agaataaact taaagat
//
LOCUS       DAMJVI010000268          435 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.274, whole genome shotgun sequence.
ACCESSION   DAMJVI010000268 DAMJVI010000000
VERSION     DAMJVI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.274"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(22..315)
                     /locus_tag="PC545_003800"
     CDS             complement(22..315)
                     /locus_tag="PC545_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458469.1"
                     /translation="MISMIMMLIGVLFIVFGIILTAVEIKGKMPGFYGVGQNAVRKNA
                     IGIIAAGIALIFFRNGLDSIDSILTYGVFLVIELVVIGLYFLIRIKYDKDGNL"
BASE COUNT          169 a           79 c           24 g          163 t
ORIGIN      
        1 aaaatattat taatttatac tttaaagatt cccatcttta tcatatttta ttcttataag
       61 aaaatataat cctattacta ctaactctat cactaaaaat actccatagg taagaattga
      121 atcaatacta tctaacccat ttctaaaaaa aattaatgct atacctgctg ctataatccc
      181 tatagcattt tttcttactg cattttgccc aactccataa aaacctggca tttttccttt
      241 tatttcgaca gcagttaaaa ttatcccaaa cactataaat aatactccaa taagcatcat
      301 aatcatacta atcatctaat tacctccttc acttaaggaa atttcaattt ctaaaatcta
      361 aaataaataa tatttatttt ttataataat attgtatatc caattaacta ttttggaaat
      421 tagtaccaat aaata
//
LOCUS       DAMJVI010000269          430 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.275, whole genome shotgun sequence.
ACCESSION   DAMJVI010000269 DAMJVI010000000
VERSION     DAMJVI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.275"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..225
                     /locus_tag="PC545_003801"
     CDS             <1..225
                     /locus_tag="PC545_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDF2458470.1"
                     /translation="YYSIAKSNSKKIKEQKRNNVLRPNKKPDIDNVVKIIADSLNEIA
                     YKDDTQIVEVVASKYYSDKPRVEVILEDVI"
BASE COUNT          187 a           41 c           86 g          116 t
ORIGIN      
        1 tattactcta tagcaaaaag taacagtaaa aagattaaag agcagaaaag aaataatgtg
       61 ttaagaccca ataaaaaacc agatattgat aatgtggtca agattatagc tgattcactc
      121 aatgagatag cttataaaga tgatacgcag attgttgagg ttgtagctag taaatattac
      181 agtgataaac caagagttga ggttatatta gaagatgtta tttaaccaac ggaaaaatcc
      241 gttcgttaga ttaggtactc agtttttcat aaaaatgtga aaaagctgaa tagagaatat
      301 atcaaatggt aaaggagaga taaattatga atgaaaatat aaataaagaa ataacagtac
      361 ttggaacttt agaaatcgag ggaatgaaat ttcataacat tgagggtgga tttggagaac
      421 ataagaaagc
//
LOCUS       DAMJVI010000270          427 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.276, whole genome shotgun sequence.
ACCESSION   DAMJVI010000270 DAMJVI010000000
VERSION     DAMJVI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.276"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            188..304
                     /gene="rrf"
                     /locus_tag="PC545_003802"
     rRNA            188..304
                     /gene="rrf"
                     /locus_tag="PC545_003802"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            310..398
                     /locus_tag="PC545_003803"
     tRNA            310..398
                     /locus_tag="PC545_003803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Leu,seq:taa)
BASE COUNT          141 a           72 c           91 g          123 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgctaactc tatattttta aacttgtcaa
       61 aacttatgga tttttaatag ttttataagg caaaaaatcg aataaaaaaa gtattgacac
      121 aataagacaa agatgttatt ataatacttg tcttagaaaa aaagttaaat gaaaatttag
      181 acaataatct ggttataata gcagagagga tacacctgtt cccattccga acacagaagt
      241 taagctctct agcgctgatg gtacttggtg ggaaactgcc tgggagagta ggacatagcc
      301 aggtgatgtg ccgaagtggc ggaactggca gacgcacagg acttaaaatc ctgcgggact
      361 tacctctcgt accggttcga ttccggtctt cggcaccaca aattattgtg ggccattagc
      421 tcagttg
//
LOCUS       DAMJVI010000271          405 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.277, whole genome shotgun sequence.
ACCESSION   DAMJVI010000271 DAMJVI010000000
VERSION     DAMJVI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.277"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..215
                     /locus_tag="PC545_003804"
     CDS             <1..215
                     /locus_tag="PC545_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDF2458471.1"
                     /translation="IGSHPSFQLFHDLVTMFNISVDEYFYPAEKVAKSTARRQIETSL
                     DLLSDNELKIIQGTIDGILNSRENKK"
BASE COUNT          125 a           73 c           68 g          139 t
ORIGIN      
        1 atatcggttc acacccaagt tttcaattat tccatgattt agttaccatg ttcaatatat
       61 cggttgatga atatttctat ccagcagaga aagttgcaaa aagcacagct cgccgtcaga
      121 tagaaacatc acttgattta ttgagtgata acgaattaaa aattattcaa ggtacgattg
      181 acggtatctt gaacagcagg gagaataaga agtaatcagc ttcttgtttt ccccgttttc
      241 tttttagcca tatttcttca agacttattt gttgatctgc tttgcaggaa acaaataaga
      301 gccgacaatc tataagttac aataacctac aaattatcgg ctctgcgtct tagcgtctgg
      361 ctctttgata atagttactt ttccatgctt tatattgatt aaagt
//
LOCUS       DAMJVI010000272          396 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.278, whole genome shotgun sequence.
ACCESSION   DAMJVI010000272 DAMJVI010000000
VERSION     DAMJVI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.278"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          169 a           34 c           39 g          154 t
ORIGIN      
        1 ttgaattgtc attaaatatg ccaatagttt tatatattat tgattttatt aaaaaaagag
       61 atatacaaac taataagttg taatctctca aatataaaaa aacaataaat tagctcattg
      121 gattttttag tccataatat aattatagat tattcaccaa acaatctata gaaattgtgg
      181 tattaatgtt taaataacca actttttcta aaataacctt tataggaata tatgagttca
      241 taagtaaaga tagcgaaata agaaaccagt ttggtatgta aaatctagat ttttaattaa
      301 attatcaaga ttatatatta tagtatcaaa aatagaaaat taaaattaaa ttttttataa
      361 taaatcttat ttttggctaa caagatttat tttttt
//
LOCUS       DAMJVI010000273          387 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.279, whole genome shotgun sequence.
ACCESSION   DAMJVI010000273 DAMJVI010000000
VERSION     DAMJVI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.279"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>387
                     /locus_tag="PC545_003805"
     rRNA            <1..>387
                     /locus_tag="PC545_003805"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          114 a           78 c          113 g           82 t
ORIGIN      
        1 gtaggataag ctaagcacac tgttggttat gtgtgcccaa gcattgaggc agtcaaagta
       61 ggcaaatccg ctttgataat gctgggatgt gatggggagc gaaatttagt agcgaagtag
      121 ctgatttcac actgtcaaga aaagtctcta tcgaggttaa aggtacccgt accgcaaacc
      181 gacacaggtg ggtgaggaga gtatcctaag gccagcgaga gaactgttgt taaggaactc
      241 ggcaaaatga ccccgtaact tagggataag gggtgccacc atcaggtggc cgcagagaat
      301 aggcccaagc gactgtttac caaaaacata ggtttctgct aagtcgcaag acgatgtata
      361 ggagctgacg cctgcccggt gctggaa
//
LOCUS       DAMJVI010000274          381 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.280, whole genome shotgun sequence.
ACCESSION   DAMJVI010000274 DAMJVI010000000
VERSION     DAMJVI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.280"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(<1..>381)
                     /locus_tag="PC545_003806"
     rRNA            complement(<1..>381)
                     /locus_tag="PC545_003806"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           89 a          107 c           87 g           98 t
ORIGIN      
        1 tgtactaccc attgtagcac gtgtgtagcc ctaagcataa ggggcatgat gatttgacgt
       61 catccccacc ttcctccagg ttatccctgg cagtctctct agagtgccca acttaatgat
      121 ggcaactaaa gacaagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga
      181 gctgacgaca accatgcacc acctgtcacc aatgtccccg aagggaactc tccgattaag
      241 gagatgtcat tgggatgtca agcttaggta aggttcttcg cgttgcttcg aattaaacca
      301 catgctccgc tacttgtgcg ggtccccgtc aattcctttg agtttcactc ttgcgagcgt
      361 acttcccagg cggagtactt a
//
LOCUS       DAMJVI010000275          364 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.281, whole genome shotgun sequence.
ACCESSION   DAMJVI010000275 DAMJVI010000000
VERSION     DAMJVI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.281"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            168..284
                     /gene="rrf"
                     /locus_tag="PC545_003807"
     rRNA            168..284
                     /gene="rrf"
                     /locus_tag="PC545_003807"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            291..362
                     /locus_tag="PC545_003808"
     tRNA            291..362
                     /locus_tag="PC545_003808"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:323..325,aa:Asn,seq:gtt)
BASE COUNT          117 a           61 c           78 g          108 t
ORIGIN      
        1 attcattaag atatatgtgc agtttttaga gtgttaactc tatgtaaagt actaacttca
       61 attgatgaag tatatatcgt aaaaacaatt actatttagt acttcaaaac tattgacatt
      121 taaaaataaa gatgttaata taatacttgt cttataaata ggcaatatgt ggttataata
      181 gcaaagagga tacacctgtt cccattccga acacagaagt taagctcttt agcgctgatg
      241 gtacttggtg ggaaactgcc tgggagagta ggacgtagcc acgtgaatat tccaaggtag
      301 ctcaatggtg gagcaaccgg ctgttaaccg gtaggctgtg ggttcgagcc ccacccttgg
      361 agcc
//
LOCUS       DAMJVI010000276          304 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.282, whole genome shotgun sequence.
ACCESSION   DAMJVI010000276 DAMJVI010000000
VERSION     DAMJVI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.282"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>304
                     /locus_tag="PC545_003809"
     CDS             <1..>304
                     /locus_tag="PC545_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HDF2458472.1"
                     /translation="NVIQKIQQLVPPTHPVLGDGILVLTDIKSSPYPGASVVPDYCKA
                     TFDRRLLVGETREGVLAPIQALLDEMMKEDPELNAKVSYAVEKADCYTGNTIESER"
BASE COUNT          119 a           44 c           55 g           86 t
ORIGIN      
        1 ctaatgtaat ccaaaagata caacaattag tacctccaac tcatccagtt ttaggtgatg
       61 gaatattagt tttaacagat ataaaatctt ctccatatcc aggagcatca gtagtgccag
      121 attattgcaa agctactttt gatagaagat tattagttgg agaaacaaga gaaggcgtat
      181 tagctccaat acaagcttta ttagatgaaa tgatgaaaga agacccagaa ttaaatgcaa
      241 aagtttctta tgcagttgaa aaagctgatt gctatacagg aaatactata gaaagtgaaa
      301 gatt
//
LOCUS       DAMJVI010000277          302 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.283, whole genome shotgun sequence.
ACCESSION   DAMJVI010000277 DAMJVI010000000
VERSION     DAMJVI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.283"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>302
                     /locus_tag="PC545_003810"
     CDS             <1..>302
                     /locus_tag="PC545_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015994384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458473.1"
                     /translation="RQINELINKNRKRFKDGKDILDLLGVGLDDTKIKELGFTQQSIN
                     SYRGLKNKGLLSGIYILSERGYAKLLKILEDDIAWELYEKLVDGYFSMRKELNNPL"
BASE COUNT          126 a           22 c           56 g           98 t
ORIGIN      
        1 agacagatta atgagcttat aaataagaat agaaaaagat ttaaagatgg aaaagatata
       61 ttagatttgt taggtgtcgg tttggacgat accaaaataa aagaattagg atttactcaa
      121 caatcaatta attcttatag agggttaaaa aacaaagggt tattatctgg gatttatata
      181 ttatctgaaa gaggttatgc aaaattatta aaaatattag aagatgatat agcttgggaa
      241 ttatatgaga agttagttga tggatatttc tctatgagaa aagaactaaa taatcctctt
      301 tt
//
LOCUS       DAMJVI010000278          279 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.284, whole genome shotgun sequence.
ACCESSION   DAMJVI010000278 DAMJVI010000000
VERSION     DAMJVI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.284"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT           86 a           38 c           19 g          136 t
ORIGIN      
        1 atttttttat atttatttga ctttctttta attctttagg atggatttaa attttctata
       61 aaaatcatac tattttagct gtttacttgc ttatttaata gttttatttc tttgattaaa
      121 tatctttcag aatctaagcc agatgtctca aaaaaactga tttataatat aatcattttt
      181 tctgctattc ttatttttcc aattttagtt tcatattaaa atatactttt catagacact
      241 acattctttc atactcattt tcacatttaa ctatacaag
//
LOCUS       DAMJVI010000279          270 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.285, whole genome shotgun sequence.
ACCESSION   DAMJVI010000279 DAMJVI010000000
VERSION     DAMJVI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.285"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            84..260
                     /locus_tag="PC545_003811"
     CDS             84..260
                     /locus_tag="PC545_003811"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458474.1"
                     /translation="MNLKKKEKIVKPVLKKLTEIVLNILSKKILNYAIYAISVLIVTK
                     VVINIDLYISILYY"
BASE COUNT          125 a           17 c           34 g           94 t
ORIGIN      
        1 ttaattattg actaaataat aaatactgta atataatgga aatacagttt aataaatata
       61 aaaagagaaa gagggaattt taaatgaatt tgaagaaaaa agagaaaata gttaagcctg
      121 ttttaaagaa gcttacagaa atagtattaa atatattatc aaaaaaaata ttaaactatg
      181 ctatatatgc aatttctgtt ttgatagtaa caaaagttgt tataaatata gatttgtaca
      241 tatccatatt gtattattag atatcttata
//
LOCUS       DAMJVI010000280          237 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.286, whole genome shotgun sequence.
ACCESSION   DAMJVI010000280 DAMJVI010000000
VERSION     DAMJVI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.286"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
BASE COUNT          105 a           12 c           31 g           89 t
ORIGIN      
        1 tatttaaata aaaaaatttt aataagtatt ggtaatatag tttttaggga tgattatttt
       61 atgtaaaaag ttgttatttg taaagataaa tatagaaaat attataagta ttgatttagt
      121 gataatctaa aaggtgagaa ttgtaataaa aagagaacat atccttcaaa tggatgtaaa
      181 cttataaata acattattac aagagattta ttatacatat actgcaacta ttaatta
//
LOCUS       DAMJVI010000281          227 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.denovo.287, whole genome shotgun sequence.
ACCESSION   DAMJVI010000281 DAMJVI010000000
VERSION     DAMJVI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.denovo.287"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            <1..>227
                     /gene="tuf"
                     /locus_tag="PC545_003812"
     CDS             <1..>227
                     /gene="tuf"
                     /locus_tag="PC545_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDF2458475.1"
                     /translation="ELFEQIDEYIPAPERDTDKPFLMPVEDVFSITGRGTVATGRVER
                     GVLKVQDEVELVGLTEAPRKVVVTGVEMFRK"
BASE COUNT           97 a           30 c           56 g           44 t
ORIGIN      
        1 gaattattcg agcaaataga tgagtatata ccagctccag agagagatac agataaacca
       61 ttcttaatgc cagtagagga cgtattctca atcacaggaa gaggaacagt tgcaacagga
      121 agagtggaaa gaggagtact aaaagtacaa gacgaagtag aattagtagg attaacagaa
      181 gcaccaagaa aagtagtagt aacaggagta gagatgttca gaaaatt
//
LOCUS       DAMJVI010000282        38282 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.027, whole genome shotgun sequence.
ACCESSION   DAMJVI010000282 DAMJVI010000000
VERSION     DAMJVI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38282
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.027"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            629..1831
                     /locus_tag="PC545_003813"
     CDS             629..1831
                     /locus_tag="PC545_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDF2458476.1"
                     /translation="MKTSILIALIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGVVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            1836..2606
                     /locus_tag="PC545_003814"
     CDS             1836..2606
                     /locus_tag="PC545_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458477.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSGIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYESYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSEMSDIELFNVNSNLYKYKKEAVNQEIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNSDYEVTVSRSKVKEFRLLMHI"
     gene            2752..4173
                     /locus_tag="PC545_003815"
     CDS             2752..4173
                     /locus_tag="PC545_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDF2458478.1"
                     /translation="MVSFLGSIVVLIVGYFVYGTFVEKVFGTNDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGLYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            4439..4921
                     /locus_tag="PC545_003816"
     CDS             4439..4921
                     /locus_tag="PC545_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HDF2458479.1"
                     /translation="MDTKKYKDKLLKERENLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGICDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            4976..5755
                     /locus_tag="PC545_003817"
     CDS             4976..5755
                     /locus_tag="PC545_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDF2458480.1"
                     /translation="MKKIIWGICICLLSIVGVYTYNHNTHNQDSSKEAYSLNDNIKDD
                     YEDVIIKKGNEDEKILALTFDDGPDEDFTPQVLDILKKNDVKATFFVVGEKVEYNKEL
                     LKRQYDEGHEIGNHTFTHINVAKNSYSSVEKEITDTQNIIKSVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKGMNIILWSNLDPRDWSNPGVDAIINTILTKVQNGNIILLHDYNTKRN
                     DKSQTIQALEVVIPKLKERGYKFVTVSELIQHLDKNKQVQK"
     gene            6065..6511
                     /gene="lspA"
                     /locus_tag="PC545_003818"
     CDS             6065..6511
                     /gene="lspA"
                     /locus_tag="PC545_003818"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDF2458481.1"
                     /translation="MLYILIIILLIGLDQLSKIWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHIFGRLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNIADVFVVVGTVFLCIYVLFFESKSR"
     gene            6480..7427
                     /locus_tag="PC545_003819"
     CDS             6480..7427
                     /locus_tag="PC545_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDF2458482.1"
                     /translation="MFYFLKVKVGDIMDEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKVEKARYLVKEEDNIVIEIPEPKVLEVLPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKEDKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRARLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            7787..8320
                     /gene="pyrR"
                     /locus_tag="PC545_003820"
     CDS             7787..8320
                     /gene="pyrR"
                     /locus_tag="PC545_003820"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDF2458483.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDITLYRDDLEKIHVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            8604..9815
                     /locus_tag="PC545_003821"
     CDS             8604..9815
                     /locus_tag="PC545_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDF2458484.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKVVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFSMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILRLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAVILLIGLGTTYLSNKGISVGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEEE"
     gene            10114..11766
                     /locus_tag="PC545_003822"
     CDS             10114..11766
                     /locus_tag="PC545_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDF2458485.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYTYLTESYSYISQSENNIETCSHSKEKSLLNAPDKFEEHLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEDMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRADFEYQEYVKEKKRLEKALLYKKALS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKVSKEMNIK
                     MQDGMEIISKNLVEVKDMTLKLENKLLLDNVSFKIKRGKKIALLGDNGCGKSTLIKEI
                     LADKNDNIKINNKVKVGYFDQNQSLLDEEKSVLYNTKVNSSFDESFIRINLSLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLVFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDAKIREFDCNYDEYTISRNKKTPSRENQIKKENLLV
                     LENRLTTVISMLSIEKDNLKKELYESEYNELLKQINKLKNSF"
     gene            complement(11981..13756)
                     /locus_tag="PC545_003823"
     CDS             complement(11981..13756)
                     /locus_tag="PC545_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HDF2458486.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTVKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDDISKDLFIKNLEELEGPIFKSIYSKFLGISPIIAKEICYRAG
                     VNQNAIIKDISDEQFDALHKVFCNLFNDINSNKYSPCIIIDKKVDKVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNDVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFVSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
     gene            14052..15086
                     /gene="ltaE"
                     /locus_tag="PC545_003824"
     CDS             14052..15086
                     /gene="ltaE"
                     /locus_tag="PC545_003824"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDF2458487.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDESGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVSLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGNKEFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            15373..16203
                     /locus_tag="PC545_003825"
     CDS             15373..16203
                     /locus_tag="PC545_003825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDF2458488.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKVNLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDRELKVTSMLM
                     GVPHTVVIVDELDVDDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            16207..17088
                     /locus_tag="PC545_003826"
     CDS             16207..17088
                     /locus_tag="PC545_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDF2458489.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            17118..17735
                     /gene="gmk"
                     /locus_tag="PC545_003827"
     CDS             17118..17735
                     /gene="gmk"
                     /locus_tag="PC545_003827"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDF2458490.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKAAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            17738..18004
                     /locus_tag="PC545_003828"
     CDS             17738..18004
                     /locus_tag="PC545_003828"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDF2458491.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKTKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            18009..19208
                     /gene="coaBC"
                     /locus_tag="PC545_003829"
     CDS             18009..19208
                     /gene="coaBC"
                     /locus_tag="PC545_003829"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDF2458492.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVKPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GTGKLANPDIIVEEVLTNLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAIARGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILIGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            19447..21972
                     /gene="priA"
                     /locus_tag="PC545_003830"
     CDS             19447..21972
                     /gene="priA"
                     /locus_tag="PC545_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDF2458493.1"
                     /translation="MNNILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSDVI
                     KIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIDSKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQEAVDEIKSNMFVDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKSGEYKLINIKSRANDKPLPNIEVVDMKDELDKGNRSIFSL
                     KLQKEIRYAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DEILNKFKDKEADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVIKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNINVSIDINPNSVL"
     gene            21990..22430
                     /gene="def"
                     /locus_tag="PC545_003831"
     CDS             21990..22430
                     /gene="def"
                     /locus_tag="PC545_003831"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDF2458494.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHEIDHLDGILFVDKIEK"
     gene            22445..23374
                     /locus_tag="PC545_003832"
     CDS             22445..23374
                     /locus_tag="PC545_003832"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDF2458495.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDKIKSLNPDVIVVVAFGQILPKEILEIPKLG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDVGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            23384..24136
                     /locus_tag="PC545_003833"
     CDS             23384..24136
                     /locus_tag="PC545_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HDF2458496.1"
                     /translation="MTDIKKYLTSVGILILILGILIGVVNFFIISLTQIFSLMINIII
                     ISLILIILCSTVVTYRVIKGKYVNSNIMKINFNIVSGLFPIISFIASSFGMSKSDIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCKECGRCNIGELI
                     KLKEKTNVKIFVATGGTLARKIIMDTKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMMDIEKAIGFLTGKNILVCN"
     gene            24205..24906
                     /locus_tag="PC545_003834"
     CDS             24205..24906
                     /locus_tag="PC545_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDF2458497.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            24968..26293
                     /gene="rsmB"
                     /locus_tag="PC545_003835"
     CDS             24968..26293
                     /gene="rsmB"
                     /locus_tag="PC545_003835"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDF2458498.1"
                     /translation="MDAREVGFKVLCDIEKNDNYSNIAINKHFKNLEISDMDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANNEKPSIYIRTNTLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEGSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLINSTVNRLGLKNVFVERFDASEIDENSISKFDYVLADVPCSGFG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASKYVKVGGTLVYSTCTIQDVENINIVTS
                     FVEENHNFEFVPIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            26301..27332
                     /gene="rlmN"
                     /locus_tag="PC545_003836"
     CDS             26301..27332
                     /gene="rlmN"
                     /locus_tag="PC545_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDF2458499.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     DDMNNIPKSLRNKLEEVSCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIEEILNACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            27352..28104
                     /locus_tag="PC545_003837"
     CDS             27352..28104
                     /locus_tag="PC545_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDF2458500.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            28121..30136
                     /gene="pknB"
                     /locus_tag="PC545_003838"
     CDS             28121..30136
                     /gene="pknB"
                     /locus_tag="PC545_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDF2458501.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPTLAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     DDEDEEEEEIMRAKKNQRPKSTPSKRTKKKKKKQESPKSRRRLKVIAAVLILILCAQV
                     FLAYKFLFAGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCII
                     SQTPEGGSTNVKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYE
                     YSSIYKEGTVLSQSPGAGSSRAQEGDEVNLYVSKGSEKSNTQTPTVPDKKPTTPENNT
                     PTEPGSNSGNSGGNSGGSNSGNSGGNSGNNGSNSGGSNSGNSGGNSGGSNSGDNGGSS
                     GGSNSGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGAGANIGKTE"
     gene            30244..30981
                     /locus_tag="PC545_003839"
     CDS             30244..30981
                     /locus_tag="PC545_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDF2458502.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTILNEKNFSVKLFKANISNREDVEDMVDYCIKEFGGLDVLVNNAGVSQDKLFTDITD
                     EDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDALVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            31079..31963
                     /gene="rsgA"
                     /locus_tag="PC545_003840"
     CDS             31079..31963
                     /gene="rsgA"
                     /locus_tag="PC545_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDF2458503.1"
                     /translation="MLEGKIIKGISGFYYVDTYNGIYECKARGIFRKQKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKNIYELSGYKVIPVSNITKLNIDKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVTNGEISKERY
                     DSYIQLLHEIRQNNSRRY"
     gene            31968..32639
                     /locus_tag="PC545_003841"
     CDS             31968..32639
                     /locus_tag="PC545_003841"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDF2458504.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPVDTIKHVLSDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVAEVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            32648..33289
                     /locus_tag="PC545_003842"
     CDS             32648..33289
                     /locus_tag="PC545_003842"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDF2458505.1"
                     /translation="MKICIVLNGEIEDYEVTRDIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     NGDAKGVTLTGLEYPLDNYYMKYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            33420..34049
                     /locus_tag="PC545_003843"
     CDS             33420..34049
                     /locus_tag="PC545_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDF2458506.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSEHKEIELSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERMNLLNECSLKECDYLSENGAKLFDDIEDTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFIDFECPGNTGLHKGENNKLIIERNKLINPI
                     YVGDTQGDANSAKFVGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            34104..34760
                     /locus_tag="PC545_003844"
     CDS             34104..34760
                     /locus_tag="PC545_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDF2458507.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVVIKRRDLMEKIADVITKKQQEILVEMDKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            35175..35891
                     /locus_tag="PC545_003845"
     CDS             35175..35891
                     /locus_tag="PC545_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458508.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIVDIMMPKMDGITLTMKLRENYNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVGNKEQKVESDDSVFAIGGLELNENTKEVSIDG
                     KYIKTTPIEFKILSLLMRNAGRVFSADEIYERVWNENAVNTDTVMVHVRNIREKIEID
                     PKNPKYLKVVWGVGYKIEKI"
     gene            35866..38199
                     /locus_tag="PC545_003846"
     CDS             35866..38199
                     /locus_tag="PC545_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2458509.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQASAKGGA
                     ENPFEQESFVDSIYKGSYVLYHNMKEEQEGKMIQPSKLFINEETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNENFENWEHFIKASGKNLKYYLVDKESNFEFFNDNKELGVLD
                     TTKNDASKENSNENQSKNTEMNDEERQKQITDIKENYRFYVVMNFDKDGKVNIVDSYG
                     VNQKIINQMLLSKAREDLMDTSLGGNSISYKLSPIKNITLVYAVPKVISKGDSVEYGY
                     GNTYFDDGIISYISNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIALAIIQGLVDLYPTVIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIREYFTKHTVTGMFFVWLKKAGKAVINQIRTINLK
                     EKPNKTIVTILGINLLIIVIMCSMWFFGIILALIYLVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLDVNMEKDLGFFNPIKDELGNIQTGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKANSGD
                     VYLNIVNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVNIMLKNMSASEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
BASE COUNT        15309 a         3789 c         6957 g        12227 t
ORIGIN      
        1 ataaaaattt tctggaattt ttattcttat acgtgataaa aatagttata actatgtatt
       61 tcagaaaata aaatttatag gtattttttt tagaagaatt atttaacaat aattatagaa
      121 gtaactttca aggttgacaa attattatgg taaatataca ttataataat ttattgtcaa
      181 acatattatt ttaaatatgg gatttattta ctttatcgat atacggctat ataataaata
      241 tataacagag agatgataat gtgtgtttaa cacaattaaa tcaaatgaga tatttgaaca
      301 aagaatagta taaaattttt aaagaaatat gttgtatgag taagaatcta cataatattg
      361 tattatatga tattcgaaag tattatttct aagaaaagag atttctaaag tataaaatta
      421 aatactagaa ttgtaaagtt aataataact acaagttatt acaagcttat gctagctagt
      481 attagttgtt aatagaagta caactatttc ttatttaatt aaaagattaa atgtggaaag
      541 ttttgtgata ggattacttc aaatatgtaa tttataacaa ccataataaa tttagagtgt
      601 gtaaaaattt attttttaaa gggagattat gaaaacatca attttaatcg cattaataga
      661 aaagacaagc cttattgtag tattgtttct tctaataaca aagttaaaaa tatttaagca
      721 gatatttcaa aaggaagaat atagttttaa tgacttggtc tgtatagcat tagtttttac
      781 ttttttagcc atatttggaa cttatagtgg tataaattat atggggtcaa tagtaaacac
      841 tagaataata tctatagtat caggtggcat tttatttggt ccgatggtag gtattacagc
      901 aggagttttt tcagggattc atagatattt tatggatatt ggggggataa catctgttcc
      961 atgtctttta tcaagtatac tagcaggtgt attatcagga tttctatata agaggatacc
     1021 aaaacaacat agagtaatgt atggaatatt ggtaggcatg atttctgaaa gttttactat
     1081 attacttata tacttaatat catatccaca ttctttagca attcaaataa tcggtggaat
     1141 atatttacct ttaatagttg ggcagattgg aataggattt gttatatcta tagtagaggg
     1201 gattgagaaa gataaaaaag atatagaagc gagaaataaa gcagaaataa aagcacttca
     1261 aagacaaatt aatccacatt ttttgtttaa ttccctaaac acaatagctt catttataag
     1321 atttagtcca gataaggcta gagagttaat aataaattta tcaacttatc taagatataa
     1381 tcttgaatat agtgataact taattgatat aaataaagag attgaacaag ttaaatcttt
     1441 tgtagagata gaaaaagcta ggtttggaga attactaact gtatcttatg atatagaaga
     1501 tgtaaatata aagattccaa gtttgattat acaaccatta gtggaaaatg caataataca
     1561 tggtatttta gaaagtggta gagctggtgt agtgaaaata tccataaaaa agttaccgta
     1621 ttcattagaa aatactgtga gaatatcaat agaggataat ggtataggta taagtgagga
     1681 aattataaat aatgtatatc aagataatat gccagaaaat aagattgggc tttataatgt
     1741 acatttaagg cttaaactga tgtatggaag agggcttaat ataagaagaa tagataatgg
     1801 gactttgata gtattttatg taaaggagta gaagtatgag agctataata gtggaagatg
     1861 aatttccagc aagaaaggaa ttgagatatt ttattgaaaa taaaagtgga atcgaggtag
     1921 taagtgaatt taccaatggt atagaggtgt tagactttat tcaggaaaat aaaatagatg
     1981 taattttttt agatataaat atacctcatc ttgatggaat gctacttgca aaaacattaa
     2041 atcagtttaa atcaagacct aaaattgttt ttataacagc atatgaaagt tatgcagtag
     2101 atgctttttc tttggatgta tttgattata tattgaaacc atattctgaa gagagaatta
     2161 tttctatgct gaataaatta gaaaaaagtg agatgagtga tattgaacta tttaatgtaa
     2221 atagtaattt atacaaatat aagaaggaag cagtaaatca ggagatagaa gaaataacac
     2281 ataaaattag cctttggaaa ggtgataaat tagttgtaat agatattgat gatatatatt
     2341 actgtgaagc taatgaaagg cagacattta tatatactga gaaagaaaag tttatattaa
     2401 aagaaggcat atcagaagtc gaaaatctta taaatgataa aacatttttt agaacacata
     2461 ggtcttatat cgtaaactta accaaagtaa aagaaattat accatggttt aataatacat
     2521 acatattaaa attaaaaaat agtgattatg aagtaactgt aagtagaagc aaagtaaaag
     2581 aatttaggct gttaatgcat atataaagtc atttcatgta cttaaatatg cacttaatgt
     2641 aattaatatt tatattagca taaaaaagta ttactatttg aactggaaat agttttctta
     2701 aactcataaa aatttataaa gcaattatat aaaaactaag gggatgaatt tatggtaagc
     2761 tttttaggtt caatagtagt acttatagta ggttattttg tctatggtac ttttgtagaa
     2821 aaggtttttg gaaccaatga caaaaatcaa actccagcta tagcttgtag ggatggagta
     2881 gattatgtgc caatgaaatg gaagagaatc tttttaattc agtttctaaa tatagcaggg
     2941 ttaggtccga tatttggagc tatacaagga gcattatttg gaccatcagc atttttatgg
     3001 attgtgtttg gaacaatctt tgctggtgga gtacacgatt ttgcttcagg atatctatca
     3061 atgaaaaaca aaggtacatc tgcatcagag ttggtaggcc tttatctagg aaatggtgca
     3121 aaaatagcaa tgagaatatt tagtgttatt cttttagtat tggttggagt tgtatttgta
     3181 acaggtccag caggattatt aaagacattg acaggtgtaa acgcacaaat atgggttgga
     3241 gtgataatac tttattatat tatggctact gttttaccaa tagataaatt gattggtaaa
     3301 atatatcctt tatttggagc agcattatta attatggcag taggtgtagc tggaggatta
     3361 ataataaagg ggtataacat accaaatata aatttacaaa atatgaatcc aaatggaaca
     3421 cctttattcc catacttatt tataacaata gcatgtggag caatatcagg ttttcatgca
     3481 actcaatcac cacttatggc gagatgtgtt gagaaagaga gtgaggcaag accagtattt
     3541 tatgggtcaa tggttgcaga aggtataata gctttagttt gggcagcagc agccatgtca
     3601 tatttccatg gcatacctca acttaatgtg atatttaatg atggtggagc agcaacagta
     3661 gtaaatacag tatctgttgg attaatgggg cctattggag gagctttagc tatacttgga
     3721 gttgtggcat gtcctataac ttctggagat acagcattta gaagtgctag gctgacaata
     3781 gcagatgcaa tgaattataa tcaagatgca gtcaaaaata gatttttaat agctttacca
     3841 ttattcgcag ttggtgttgc tcttacattt atagatttta atataatttg gagatatttc
     3901 tcttgggcaa atcaaacact tgcaatgata atgttgtgga ctggttctgc atatttagtc
     3961 aaagcaaata aaaaccatta tataactaca ttaccagcac tttttatgac tgtagtaaca
     4021 tttagttata taatgcaagc aaaagaagga tttagactac cagttaatat atctaatgga
     4081 ataggtgtta ttgtagcaat aattcttggt cttttattct ttaaaaaagc aaaacatatt
     4141 aaagaacaaa ataatcataa attggcaagt tagtaaaata ataactataa aaattaaaaa
     4201 attgtattta aattatgttc aaagaagaga ctatctcaaa atagagataa gtctcttttt
     4261 tattgctgaa aggataaaaa tatatttgat ttcatataaa aaagagggtt aaatgaacta
     4321 aaaatttcat tttgagacat tccatttttc gaatcaaatc ttatttttat tgtatgttta
     4381 atttactttt ccacatacta tttttatgat gtaaatatta aaattagagg tgttaaaaat
     4441 ggatactaaa aaatataaag ataaattatt gaaagaaaga gaaaatttaa ctaatttagt
     4501 agaggatatg aaagataata ctttatttgg agatacaact aaacatacaa gtgaaaagta
     4561 ttcatcagga gaattatcta gttatgataa tcatatagga gacatgggaa cagatttata
     4621 tatgcaaaat atgcaaaaca gtctaataaa tcatgaagag ggaagattgt atcaaataga
     4681 cttagcatta tctaaaatag agaatggaac atatgggatt tgtgatttat gtcataatca
     4741 aatagattta gaaagattgg atatattgcc agatacaagt ttatgtaatg attgtgcaaa
     4801 gaaagaaaat aatttattag gtgatgcaat gcaaaaccct gatgtagaag attctaactt
     4861 ttattcagaa gacctaacaa atttaactga tttaaataaa aatggaatat tgagagatta
     4921 aaaaactgac attataaaat atattgctag aatgaattag aaaagaggta atgacatgaa
     4981 gaaaataata tggggtatat gtatatgttt attatctata gtaggtgtat atacatataa
     5041 tcataataca cataatcaag atagttcaaa agaagcatat agtttaaatg acaatataaa
     5101 agacgattat gaagatgtaa taataaaaaa aggaaatgag gatgaaaaaa ttttagcatt
     5161 aacttttgat gatggtccag atgaggattt tacaccacaa gtacttgata tattaaagaa
     5221 aaatgatgta aaagcaactt tttttgtagt tggagaaaaa gtagaatata acaaagaatt
     5281 attaaaaaga caatatgatg aggggcatga aatagggaat catacattta cacatataaa
     5341 tgttgcaaaa aatagttata gtagtgttga aaaagaaata acagatacgc aaaatattat
     5401 aaaaagtgta ataggagtag aaccaaagat atttagacct ccatatagag ccatgagtaa
     5461 aagtgtctgt gatattatag tttctaaagg catgaatata attctttggt caaacttaga
     5521 ccctagagat tggtctaacc ctggagtaga tgcaattata aatactatcc ttacaaaggt
     5581 ccaaaatggt aatataatac ttttacatga ttataacact aaaagaaatg ataaatcaca
     5641 aactatacaa gctttagaag tagtaattcc taaattaaaa gagagaggat ataaattcgt
     5701 aacagtatct gaacttatac aacatttaga taaaaataag caagtgcaaa agtaaaaata
     5761 tttgtttgaa gattagtggt gattgtaagt ttaaattaca gacatcatta atctttaatc
     5821 tataaaatac acaaaaaata ttgattagta tggtattttt aaatttttta tacataatga
     5881 tatgatttta aatatgtaga aatctacaat ataaaaaaag tagtactatt ttaaattttg
     5941 ttctagcttt attaagaaaa tacaatttta ataaagttgc tctttagagc attttttttg
     6001 tataataata atagtgtgct tcaaataaaa ttggttttta aataatttat agggggaaaa
     6061 atcattgcta tatatattaa taataattct actcataggt ttagaccaac tgtctaaaat
     6121 atgggtattg aataatttgg tggatgtatc aacaatacca ataataaata atgtatttca
     6181 tttaacttat gtcgaaaata gaggtgcagc atttggatta ttacaaaata atcaatggat
     6241 atttataatt gttgcattac ttgcaacagt atttggacta tactatctta atacaaggaa
     6301 agtacatata tttggaaggt tgggaattat attaattata tctggtgcat tgggaaatct
     6361 aattgataga gtacgattag gttttgtagt agattacttc gactttagaa ttatatggga
     6421 atatgtattc aatatagctg atgtatttgt agttgtagga actgtgtttt tatgtatata
     6481 tgttttattt tttgaaagta aaagtaggtg atataatgga tgaagtaaaa gagtttttag
     6541 tacttgaaga ggaagaagaa gttagacttg atatttatct agcagaacaa ttaggtgata
     6601 tgtcaagaag ttatatacaa aaaattataa aagatggaaa agtaaaggtt aataataaag
     6661 tagaaaaagc tagatatctg gttaaagagg aagataatat tgttatagag ataccagaac
     6721 ctaaagtatt agaagtttta cctcaagata ttccaataga tattgtatat gaagatgatg
     6781 atgtcttaat aataaataaa cctcaggata tggttgtcca tcctgctcct ggaaactatg
     6841 aaaatacgtt agtaaacggg attttatatc attgtaaaga taagttgtca tcaataaatg
     6901 gcgttattag acctggtata gttcatagaa tagacaaaga tacttctggt cttcttatga
     6961 tagctaagaa caattatgca cataattttc tagcagaaca gttaaaggaa catactataa
     7021 ctagggagta tgaatttata tgctatggtg tggttaaaga agataaaata actgttgata
     7081 aacctatagg gagaaatcct aaggatagat taaaaatggc tgtagtaaaa gatggaaaaa
     7141 atgcaatcac tcactttgaa gttgtagaaa gatttgataa gtttacacat atgagagcta
     7201 ggcttgaaac tggtagaact catcaaataa gagttcatgc tttgtctata aatcacccac
     7261 ttttaggtga ttatgtatat ggtccaaaag aaactaaatt taaattaaaa ggacaaactt
     7321 tgcatgctaa gaagttggga tttatacatc ctactactaa ggaatatatc gaatttaatt
     7381 cagaattacc tgagtatttt aaggaagtta ttaaaaagat aaaataacta tttgtataat
     7441 acttataaat cattttttgt ttttatataa gtaaataaat ttagtgaaat ttaaataaaa
     7501 tatcttatat taaagtattt tgtttaaaaa agcttgaaaa atttgaaata taaatatata
     7561 atttgatttg atagattaaa aaattatctt taaacgatac agagatatcg taagacaatt
     7621 gctaaattgc actgtatatt ttttatataa gagcttcttg tcttgtgatg agaagttttt
     7681 ttgttgttta aattgaaatt ttaaataaaa ataaatttat aaaaaattaa aagaaaggag
     7741 tagatttgtc aaatagatat attatatctt tgacaataat tatgatatgg tagaaaaagc
     7801 tcaattaatg gatgaaaagg caatagcaag agcaattact agaataagtc atgaaataat
     7861 tgaaagaaat aaaggtgtag aaaatctagt tttagttgga ataaagacta gaggtgtgcc
     7921 aattgcaaat agaatttcta agaaaataga acaaatagaa ggaacaaaag tagatacagg
     7981 agatattgat ataactttgt atagagatga tttggaaaaa attcatgttg aacctgtagt
     8041 taaaggaact tatttagatt ttaatgtaaa tgataagaca gtagttttag ttgatgatgt
     8101 tttatacact ggaagaactg tgagagcttc actagatgct attatagata taggaagacc
     8161 taaatcaata caattagcag tgctagtgga cagagggcat agagagttgc ctataagagc
     8221 agattatgta ggaaaaaatg tacctacttc tagacatgaa attatatctg tcagtttatt
     8281 agaaattgat ggagaagatt cagttacaat caaagaataa ttatttttaa gtattatata
     8341 aaagcttagc attgaatagt cagggaggag gataaagctt acatagattg cacaaaataa
     8401 atattataat tttatggatg tgagtcttaa tgtagttttt attctccttt atttgagttt
     8461 ttgatgtcgt aaaatgtaaa ataatgataa attatataat aagtatatag aggttaaaaa
     8521 atagaatcta gggaatgata attaattaaa agatattaat taaacagagg atttatttat
     8581 atataataat tttggaggtt tttatgaaaa aaacgataat gtctttacaa catctattag
     8641 ccatgtttgg agcgacagta ttagttccca ttttgacagg ttttaatccg tcagttgcaa
     8701 ttttctgtgc tggtgttggt acattgatat ttcatttttg tacagaagga aaagtacctg
     8761 cctttttagg ttcaagtttt gcgtttatac cagttatact agcagctaaa gaggcatatg
     8821 gtggagattt agcatatgct caaggtggta taatagtagc aggattaatt tatattataa
     8881 tgtcgattat agtaaaagta gttggtgtaa ataaaattaa attatatttt cctgcacaag
     8941 ttacaggagc tatgatagta gttataggat taaatttatt gccaacagct tttagtatgg
     9001 cttcagccaa ttttgtgata gcatttataa ctttagcaat agcaatttta accaataagt
     9061 ttggtagagg atttataaaa caattaggaa tattaatagc agttttttct gggtatataa
     9121 tctgtttgat attaagatta gtagatataa ctactattac agaagcaagt ttatttgcaa
     9181 tacctaactt tacagttcct aagttcagtt taggagcaat agtgattata tctccagttg
     9241 tattagctgt atttatggaa catataggag atatgactac taatggtgct gtagtaggta
     9301 aaaactttat agaaaatccc ggattaaata gaacattact tggggatgga tttgcgacta
     9361 tagttgcagg gtgtttagga ggaccagcca atacaactta tggagaaaat acagcagtac
     9421 ttgctataac taaaaactat gacccttcaa tacttagacg tacagcaata tttgctatat
     9481 tgcttgcatg tgtaggcaag tttggtggat ttcttcaaag tataccaggt tcagttatgg
     9541 gtggaattag tataatgcta ttttcgatga taacttatgt aggattaaaa actataaggg
     9601 acagttcttg tgtagaaagt aaaataaata tattaatcat agcagttata ttattaatag
     9661 gtcttggaac aacatattta tctaataaag gtatttctgt tggaatccct ataacaagta
     9721 cagtaaagat aactggtctt agtttagctg caattgttgg aattgtatta aacagaattt
     9781 taaataatca ggactttaaa gttgaagaag aataaattta aagaataagt tgtatattga
     9841 ataactaaaa ttagttaatt ttctatagac atttgtaatt aatgttgata taatgattaa
     9901 aaataaatga aagaaagggc aaggtaacta ggacaatgag aatataatct attttttata
     9961 atttaaagta ttgtaatttt tgaaaagcct attttggtgg ctttatttgc aatgcttaaa
    10021 ttaaggagag tagattatag tctatacgtt tatttgttat gcctatttat agaatctctc
    10081 cttatattta agtgtgttaa ggagggattt tttatgttgt tagtaaaagt agagaattta
    10141 aagaaatatt atgcagataa attaattttg gatatagata aacttgaaat attagaaaat
    10201 gacaaaatag gtctagttgg ttcaaatggt caaggaaaaa caacattatt aaaagcaata
    10261 ttgggagaaa tagaaattga tgaaggatat acttacctta cagaaagcta ttcttatata
    10321 agtcagagtg aaaataatat tgaaacatgt agtcatagta aggagaagag tcttttaaat
    10381 gctccagata aatttgaaga acacttatca ggaggggaaa aggttaagtt aaaaatagca
    10441 gatgcgctga gtaataagaa aaatatcata atagctgatg agccaacttc aaacttagat
    10501 aaaaaaagca ttggggtttt ggaagatatg tttaaaaggc atgagggagc attattatta
    10561 atatctcatg atagacgttt tctggatgag ttatgtacaa ctatattaga actagaagat
    10621 ggaaaactaa aagcttacaa aggcaattac actgattatt taatgcaaaa agatgaagaa
    10681 gtaaaaagag ctgattttga gtatcaagaa tatgttaaag agaaaaaaag gcttgaaaaa
    10741 gctcttttat ataaaaaagc tttaagtgat ggcataagaa aaacaccaaa aagaatgggt
    10801 aattcagaag ctaggttgca caaaatgggt ggtcagacta ataagaaaaa gttggactca
    10861 aatgtgaagg ctataaaaag cagaattgat aaacttgagg taaagaataa acctaaagtt
    10921 tctaaagaaa tgaacattaa gatgcaagat ggtatggaga taattagcaa aaatctagta
    10981 gaggtaaaag atatgactct aaagttagaa aataagctcc tgttagataa tgtttccttt
    11041 aagataaaaa gaggtaaaaa gatagcattg ttaggtgata atgggtgtgg aaaaagtact
    11101 ctgataaaag aaatattggc tgataaaaat gacaatataa aaataaataa caaggtaaaa
    11161 gtaggttatt ttgaccaaaa tcaaagttta ttagatgagg aaaagagtgt tctatacaat
    11221 actaaagtta atagttcatt tgatgaatct tttataagga taaacttaag cctatttggg
    11281 tttaaaggtg atgatgtcta caaaaaagta aaagtactta gtggtggaga aaaggtaaaa
    11341 atagcactat gtaaaataat attggaagat aataattttt tagtatttga tgaaccaaca
    11401 aattacttag atataaaatc tatggaagca ctggaaaagg cactgataaa tactgataaa
    11461 actatgctta tagtgtctca tgatagagta tttgtatcac atatttgtaa ctatattata
    11521 gagataaaag atgctaaaat aagggagttt gattgtaatt atgacgaata tacaattagc
    11581 agaaataaaa aaactcctag tagagaaaac caaatcaaaa aagaaaatct tctagtatta
    11641 gaaaatagac ttacaactgt tatatctatg ttatctatag aaaaagataa tctaaaaaaa
    11701 gaattgtatg aatcagaata taatgaatta ctaaaacaaa tcaataaatt aaaaaatagc
    11761 ttttagtgtt aactttttaa ttacatctat ataagaagac tgaaaatatt tgttgggaat
    11821 ataagtatag ttattaaaac agaaaagtat tttgttagaa aattaaaagt aagctaatta
    11881 gaaagtggcc ttattattgt actttataag aaggccacta tttttattta gaatgataat
    11941 gtgtagttgt ctaacattta ttttgtattt agtgcagaat ttattcagat ttaaccttaa
    12001 gcttggctac tgtttcttct tcaggagtaa cataaatagt actgttagtt tcataaataa
    12061 ccatacctgg tttagaacca ctaggttttt tcacattttt tctcttagta tagtctacag
    12121 gcacctgaga agaaagtttt gacttactaa agaatgctgc tagcatagct ccttcaaata
    12181 cagtattatc agggacttcc ttaccagcac actttattat tacatgagaa ccaggaatat
    12241 tttttgtatg catccataag tcatcattat cagcaagtct tagtgtaaga tagtcatttt
    12301 gcttattgtt tttaccaacc aatattttaa atccatcaga agacacaaat tcatgaggtt
    12361 ttgtagatgg gatagtatcc tttttactgt ttttcttttg agtcttgatg tatccaactt
    12421 tagcaagttc ttcttttata tcttgtaatt cagctaaatt ttcacagttt tcaatactta
    12481 aaattatgtt ttctaagtaa tctatttctt ccttatttaa ggaaatttga tgagatatct
    12541 cgactttagc atgtttcatt ttgttatact ttttaaagta tttttgagca ttttcagatg
    12601 gtgtcaagtt tttatttaat tctattgtta catcattaca attttcatca tagaagttag
    12661 caacttcaat actttccata cctttttcta ccatataaat atatgaagta atcaattctc
    12721 cttttatctt ataaatgtct gcattttcgg attctgataa ttcttcttct tgttttttaa
    12781 gtttattata aagtctatca agcttaacag atatactctt ctttaaatca gatgaacgtt
    12841 gattaattct atctttaata tccttagttc tatagaaatc ttccagaata cgagacatac
    12901 tatccttatt tatatatgat aaatcgctaa ataatgtaag gtttatacaa ctgaaatcaa
    12961 ctaccttatc caccttttta tctataataa tgcaaggaga gtatttgtta gaatttatat
    13021 cattgaataa attacaaaat actttgtgta aagcatcaaa ttgttcatca gatatatcct
    13081 ttatgatagc attttgattt acacctgctc tgtagcaaat ttctttagct ataatagggc
    13141 ttatacctag aaatttagaa tatatagact taaagatagg tccttccaat tcctctaggt
    13201 ttttaataaa caaatcctta gatatatcat caagagggtt gagtttgttt tgttctggtg
    13261 gcaatgaata atcatgacca ggtaaaactt gacgaactct acttatgcta aaagggactc
    13321 tttttataga atctataatt ttattttctt caccatgagt tattattatg ttgctatgtc
    13381 ttcccataat ttcaatcata atatttttaa ctgtcttttc ttttaattca tctaaagatt
    13441 caacacttat ttttattatt ctttcaaaac ctatttgaga aatttctact atatttccac
    13501 cttgtatgta ttttctaagt aacatacaaa acataggcgc ttttagaggg ttttcttttt
    13561 gataattact tgtaagataa actctaggat ttgaggcact tgcacttaaa accaatctaa
    13621 aattttcttt attatttctt atattaaaaa ttacctcatc atcttcaggt tgatgtatct
    13681 tatctatttt tccacctgta agttttgatg ataattcatc gactattgaa tgtataacta
    13741 atccatctaa tgccatttat agcacccctt tctttgacca aaatgagtca gtaaattaat
    13801 tatacacata atatacgctt tgtgactagg gggaatttaa acaatagaga ttaatattta
    13861 aattgtataa ttatttatat ttattagaga ggaaacttta tttttaaaat taataattta
    13921 aagtagatta aatataaagt aaatatagta taattattgt gtagagtata agctatgaaa
    13981 gtatatgaac tttacagaaa aaatcaattt taaggttcta tagtatataa atatgaaaga
    14041 aggttgaaaa aatgagattt atcgatttaa gaagtgatac tgttactatg cctactgacg
    14101 aaatgagaga agctatggca aaggctcctg ttggagattc aatctttaga gatgacccta
    14161 ctgtaaataa acttgaagaa ttagcagcag ctaaagttgg aaaagaagat gctattttcc
    14221 ttccaagtgg aacttttggg aatcaattag ctctatttac tcattgccta agagggcaag
    14281 aaataattat tggtaaaggt tatcacattg tgactcatga ggttggggca cctgctgtaa
    14341 tagctggagt tcagcttaga actatagatg aagacgaaag tggagctcta aatcctgaac
    14401 ttgttgaaaa ggcaattaga agagatgaca tacatgaacc acagacaggg cttatatgtg
    14461 tagaaaatgc atattcaggt ggaacagttg tgagtcttga taatttaaga gaaattaaaa
    14521 aaatagctga gaaacataac ttaccagtac atttagatgg agcaagatta tttaacgcag
    14581 cattaacttt gggtgtagaa gctaaagaaa tagcaaagtg ttgtgatagt gttatgttct
    14641 gtgtgtcaaa aggtttagct gcaccaatgg gttctatact tgctggaaat aaagaattta
    14701 tagcaaaagc tagaagaaag caaaaagtta tgggtggggg aatgagacaa gtaggtatag
    14761 ttgcagcagc tggtataatt gccattgaaa aaatgacttt aagattgaat gaagaccatg
    14821 aaaatgcaaa atatttagct tgtgaattaa ataaaattga tggcattgaa gtaaacaatg
    14881 ttaatccaga tataagtatg gtatttttta agatgagtga agatattata aaagaggatg
    14941 ttttaataaa agaatttttt gaaagaaata taaaaattaa taaaatggaa aatggtgaat
    15001 atagatttgt aactcatgta gatattacta aggaagattt agactatgtt ttaaaaacac
    15061 ttaaagaatt aataggtgta tgttaataga tatctactag aaggattata gctagttacc
    15121 agatgtttaa ttaaaaaatt catagacagt ttaaataaaa catacataaa attcatatag
    15181 aaatattaat atataaacat agaaagaact agcttaaaaa cataatataa ttaccctaat
    15241 tgtaatggca actctccctc acatcctcaa attatagttg tcattacaaa acttttccca
    15301 taaattataa gtttatggga atttttttat gttataatag tttaagcgtt aatttacgaa
    15361 ggagcgatac ttatgaaatt ttggaaacta catggaatag gaaatgattt tatagcaata
    15421 gatggaagat tcgaccagat aaatccatct gattatactg ctttagcaaa aaaagtatgc
    15481 catagaagat tttctgtagg agcggatgga ttgttagttg tgaaagattc aaaagttgca
    15541 gatgtagaaa tggtatatta taattctgat ggttcaagag cagctatgtg tggaaatggc
    15601 cttagatgtt tttgtaaatt tgtttatgat aattgtatag ttaaaaaaga aagttttaca
    15661 gtagatacat tagatggtat aaaagatata aaagtaaatc ttactaatag agaaataaac
    15721 tctataagag tcaatatggg aaaaggaagt tttatagcta aggacgttcc tgtacttaca
    15781 aataaagaac gttttgtgca agaaagtata aaggttttag atagggagct taaagttact
    15841 tctatgttga tgggggttcc tcatacagta gttatagtag atgaattaga tgtagatgat
    15901 gtttgtaaat atggaaaaga gattgaaaat aataaaatat ttcctgaaaa aacgaatgta
    15961 aattttgtaa aggtagaaga taagtataat atacatgttt atacttggga aagaggttgt
    16021 ggatatactt taggttgtgg tactggtatg actgcatcag caatagtgtg caatttactt
    16081 aatatggtag aaagtagtgt aaatgtaacg tctcaaggtg gaacagttaa aatagatgta
    16141 ggagatttct cttatatgac aggtccagca gtaaaaatat gtgaaggtat tttggaggtt
    16201 taattaatgg ctataagtat gactggtttt ggaagaggag aatacaagga tgataattat
    16261 tactttttag tagagtgtaa aacgataaat cataagtatt cagatataaa catacgtctt
    16321 ccaagaaaaa tttcattttt agaggataaa gttagaaatt tagtaaaaaa ttatgtaaaa
    16381 agaggaagag tagatttata cataaagttt gacttattag gcaaagaaga tgtaaactta
    16441 aattttgatg agggattagc atctcagtat atagacatac taaaagaaat aaaaaataaa
    16501 tttgatatta tagatgatat tagcgttatg aatgttgcaa agtttcctga tattgtgaaa
    16561 atagaagaaa aggaagaaga tgaagattta ttgtggtcaa tgttgaatca agcagttgaa
    16621 gatgcattaa tcaaattaag agaaatgaga agtgaagaag gtaaaaaatt agctgaagat
    16681 attgccatga gatgtgattt attaaaaaat catatagaag agattgaaaa atattcaagt
    16741 agtgttgtag aagattatag agaaaaattg aatttgagaa taagtgaatt actggatgac
    16801 ccaagtataa tagatgagaa tagattagca caagaagttg caatatatgc agataaaagc
    16861 agtataacag aagaaatagt tagatttaaa agtcatatag ggcaacttaa aaatactata
    16921 tttaaagacg actctatagg tagaaaaata gatttcttaa tacaagagat gaatagagaa
    16981 actaacacta taggttcgaa gtcatctgat ataaatataa ctaatttagt agtagaagta
    17041 aaaagcgaat tggaaaaaat aagagaacaa atacaaaata tagagtaaga atacaatatt
    17101 agaaggggaa tgcttaaatg tttgtgaaaa aaggattatt gctagttgta tcaggaccat
    17161 caggtgcagg aaaaggtact atttgcaaag aactacttaa agaaaatgat acaataaaac
    17221 tttctgtatc agctactaca agaaaaccca gaactggaga agttgatgga gtaaattatt
    17281 tcttcataag taaggaaaag tttgaagaga tgatagaaaa aggtgagttt ttggagtatg
    17341 ctcaaattta tgataatttt tatggaactc caaaagcggc aataatggaa tgtttagaaa
    17401 aaggacaaga tgtattactg gagattgaaa tgcaaggagc aaaacaaata aaagaagttt
    17461 gcccagaggg agtatttata tttgtactac caccatcact tgaagaatta aaaaatagaa
    17521 ttgttggaag aggtacagaa acagaagctg aaatagagaa gagatttagc tgtgcatatg
    17581 aagaaattaa aatgattaag gattatgatt attttatatt taatgaagat gtaaaaacat
    17641 ctgctaaaga aatagaaggt ataatatcat ctgaaaagaa taaagttagc agatataaaa
    17701 atattataat agagaaattt aaggaggaat tataattatg ttaaaaccat ctatcaatga
    17761 ggtactagaa aaaatagaca acagatacta tttagtgggg actgtttcaa agagagcaag
    17821 aaaattaatt gatggagaag aaccatatgt ttcaaataaa actaaggaaa agcctgtatg
    17881 tgtagcaact aaagaagttg caagtggaaa aataacatat agattattga cagaagagga
    17941 aatagagata gaagaagcta gacatcatgc tgagcaacac caacaaattt cagaagagga
    18001 gtaaggtcat gctaaaagat aaaactgttg taattggcat aagtggcggt atagctgcat
    18061 ataaggcttg tgatgttgta agtaagttaa aaaaactaaa tgcaaatatc catgttataa
    18121 tgacaaaatc agcaacagaa tttgttaaac cacttacatt tcaatcgctg agtcaaaatt
    18181 atgtagtaga agatatgttt gaagaaccaa aaacttggga agttgaacat atatcattag
    18241 caaaaaaagc agatttattc ttaatagtgc cagcaagtgc aaatgttata ggcaagattg
    18301 caaatggtat agctgacgat atgttgacaa ctactgttat ggcaacaaaa gcacaggttc
    18361 ttatagcacc agctatgaat actaatatgt atgaaaatcc aattgttcaa aaaaatatac
    18421 aaactttaaa gagctttgga tataaattta ttgaaccgga aagtggaagg cttgcatgtg
    18481 gagatattgg aacaggtaaa ctagctaatc cagatataat agtagaggaa gttttgacta
    18541 atcttactaa agagcaagat ttaaaaggtc aaagtatgat agttacagct ggtccaactg
    18601 tagagagtat agacccagta agatatttaa ctaataggtc ttctggtaaa atgggatatt
    18661 ctatagcaag agaagctata gctagaggtg ctgatgtaac attaatatca ggtcctacaa
    18721 atctttctat tccacaaaat cttaaaaaat ttgttcaaat agagtcagca caagatttgt
    18781 atgaagcagt agtatcaaat ttaagtgaaa ataaaatagt aattcaaagt gcagcagttg
    18841 cagattataa accaaaaact tattctgaca aaaaaattaa aaagaagaat gatgatttat
    18901 caatagaact aagtagaaat tatgatattg cttatgaaat aggaaaaata aaagaagata
    18961 agatactaat tggatttgca gctgaaacta atgaccttat agaacatgcc aaaggaaaaa
    19021 taaaaaagaa aaatttagat tttattgtgg caaatgattt aactaaagaa ggtgctggtt
    19081 ttagagctga tactaatatt gtaaaaataa tagatgtaga gggaaatatt actgagtatc
    19141 ctaagatgaa aaaagaagaa gtagccaatg ttattttaga taaagttaaa gcgttgcttg
    19201 agaaataaga ccttttttaa atatgatatt tgtgtatttt atcacagcta aagtggctga
    19261 aagctgcaat ttaattttag cgagtaaaaa ctcgcaaatg tcagttaaat tgtagttttc
    19321 agcatttatt ttgtcataaa gtataaaata taattaatag ttaattatta aaataaagtt
    19381 aatattaata acttgaaatt gcttataaat tctgagattt taatatcttg taaagtatat
    19441 taaaatatga acaatatatt aaatttaagt agaggtttta taatgaaaaa atatgcaaag
    19501 gtaatagtta gaagtaatac tatatataca gataatttat ttacatatca aattcctgta
    19561 tttttatctg atgtaataaa aattgggcac agaatattag taccatttgg aaaaggtaat
    19621 aaaccaacag aagcctttgt atttcaattt acagatagct tagatgaaaa aataaaaata
    19681 aaagaaatta ttgatatatt agatgaaaat ccgatattta gaaaagaaga tttagagttg
    19741 gtttattgga tgaaaaatag atatttgtgt acttatatag aatgtataaa tttgatatat
    19801 ccaaagggat acaaattaaa taattataaa gtagtcctat taggtgaaag tttaagtgga
    19861 ttaaatgata ttgaattgaa ggaaaaaata tcaactttaa gtgataaaaa tagagaaata
    19921 gtagagaaag tgattgatag taaaggtaaa attaaggttg ataagttaaa atatatacca
    19981 aacttaaata gttctttgta cacaatgaat aaaaatggaa ttataaaatt atgttgggaa
    20041 tataaaaatc acaaaaatga aaagaaagta tgttatatat ctttgagttt agaaagtgat
    20101 aagattgacg attatataga gcaaaataaa ataaatgtgg gaagtaaaca aaaagagata
    20161 ttaagttttc ttaaaaataa tgaaaatgta gagataaatg atttattgga tttattaaat
    20221 gcttcaaaac aaagtataaa ttctctaagt aagaaaaagc taataaccct agaatttaag
    20281 gattattata gagagccaaa aagtatttac aaatccgtac taaaaagtat aaaattaaac
    20341 aatgagcaac aagaagcagt tgatgagata aagtcgaata tgtttgtaga tgataaaaaa
    20401 ccatacttaa ttcatggagt aactggaagt ggaaaaactg aagtttacat ggaaataata
    20461 gaatttgctc taaatcaagg cttagacagt atattcttag tacctgagat agcattaact
    20521 cctcaaacaa tagataggct aaagagtagg tttggagatt tagttggagt gttccacagt
    20581 aaactatctg aaggagaaaa acatgacgtt tacaaagctg tcaaagcagg taaagtaaga
    20641 gtgttaattg gagctaggtc agctttattt gcacctttta attccttagg cttaataata
    20701 attgatgaat gtcatgagtc atcatataag tctgagaaga atccaaaatt taatgctata
    20761 gaagttgcta gatttatggc attaaaaaat aatataactc ttattttagg ttcagcaacg
    20821 ccctcaattg aagagtatta tagggcaaaa tctggagaat acaaacttat aaatataaaa
    20881 agtagagcaa atgataagcc tcttccaaac attgaagttg tagacatgaa agatgagtta
    20941 gataaaggaa atagaagtat atttagtttg aaattacaaa aagaaatacg ttatgcgatt
    21001 gaagaaaata atcaagtaat actattttta aacagaagag gttatgcaaa ttttgtatct
    21061 tgtagaaagt gtggatatgt gtttcaatgt gaaaattgtg atatatcttt aacttatcat
    21121 aagaaaagca atactggaag atgccactat tgtggatatg agaaagaaat tccaaaagag
    21181 tgtcctgaat gtaaaagtac ttatgttaag ccatttggag ttggaactca aaaaattgaa
    21241 gaggaattaa aatatatttt tccagatata aaaactttga gaatggataa agatacaact
    21301 tcaaaaaaag gtgctttaga tgagatacta aataaattta aagataaaga agcagatgta
    21361 cttattggaa cacagatgtt aagtaaaggg ctggattttg agaatgtaac tttagtaggt
    21421 attttgtctg cagacatgat attaaatttt ccagacttta aaagttttga aactacattt
    21481 caattgataa ctcaagtctc tggaagagca gggagagctg ataaagaggg aaaagtagta
    21541 cttcagactt atgacactga acactatgct attaagcatg ctattgaata tgattatgag
    21601 ggtttttatg aagatgagat aaaaataaga aaagcatttg gatattctcc ttttaataat
    21661 atgttaagtg tagttgttag tggtgaagat gaaagattag tgataaaaaa tataaaaaat
    21721 atgcatgcat cattgattta tttattagaa aaaaggggta taaatgattt aggatttata
    21781 ttaggtccaa atccatgttc aatatcaaaa ataaatcaaa attatagatg gcaaatactc
    21841 tttaaagatg aaaatattga aattaatctc ttaaagggta taataaaata tatatgtata
    21901 acaaaacgag atttgatatt tgataagaat atcaatgtat ctatagatat taatccaaac
    21961 agtgtattat aaatttagga ggaattataa tggctttaag acaaatagtt caaataggag
    22021 aaccagtatt aagaaaaaaa tcaaaaaagg ttgaaaaaat tgatgaaaag ataatacaat
    22081 tattagatga tatggcagaa actatgtatg atgcagatgg tgttggactg gctgcacctc
    22141 aagttggaat attaaaaaga gtagtagtaa tagatattgg agaagagctt atagaactta
    22201 taaatcctga aataatagaa acatctgggg agcaaataga tgaggaagga tgtttaagtg
    22261 ttgttggaga agcgggaaat gttagaagac caaattatgt aaaggtcaga gctttaaata
    22321 gaaatggtga aacaatagag ttagagggag aagaactttt agctagagca ttttgtcatg
    22381 agatagacca cttagatgga attttatttg ttgacaaaat agaaaagtag taggagttga
    22441 tttgatgaaa atagtattta tgggaactcc tgatatagca gttccatgtt tacaaaaaat
    22501 aatagatgag aagtatgaaa tattgggtgt tgtaactcaa ccagataaac caaaaggaag
    22561 aggtaaaaaa ctaggtatga gtccagtaaa agaactggca attgaaaata atatacctgt
    22621 atatcaacca gtaaaggcta gagataaaga atttatagat aaaattaaat ctttaaatcc
    22681 agatgtaata gtagttgtag cttttggaca gatacttcca aaagaaatat tagagattcc
    22741 taaacttgga tgtataaatg ttcatgtttc tttacttcca aaatatagag gtgcggcacc
    22801 tataaattgg gtaataataa atggtgaaga aaagactggt gttacaacta tgtatatgga
    22861 tgaaggatta gatactggag atatgatact aaagacagaa gtaaatcttg atgaaaatat
    22921 aacagcagga gaacttcatg ataaaatgat gaatatagga gcagaaactt taaaagaaac
    22981 attaagatta attgaagaag gaaatgctcc aagagaagta caaaatcatg aagaattttc
    23041 ctatgcgcca ataatgaata aatcattagg taacatagat ttttcaaaaa gtgccagaga
    23101 aatacacaat ttagttagag gagtaaatcc atggccaagt gcttatacta cctataatga
    23161 tgtcataatg aaagtttgga aaactaaagt gttagatgaa aaaagcacaa aagatgtagg
    23221 tacaataatt gatgtaagca aagatgggat aaaagtaagt actatagata atgtactttt
    23281 aattgaagaa attcagatgc cgaataaaaa gagaatgttg gttggtgaat atataaaagg
    23341 gaatactata gaaactggtt tagtattggg ttaggtgaaa agtatgacag atataaagaa
    23401 gtatttaaca agtgttggga ttttgatttt aatattagga attttgatag gagttgttaa
    23461 tttttttata ataagtttaa ctcaaatatt ctccttgatg ataaatatta taataatatc
    23521 actaatactt ataatactat gttcaacagt agttacttat agagtaatta aaggaaagta
    23581 tgttaactca aatataatga aaataaattt taatattgtc agtggattat ttcctattat
    23641 atctttcatt gcatcatcat ttggtatgtc aaaaagtgat attagaagaa tatatataaa
    23701 gttaaataat gaatatatat acagtaataa atataatttt aatccagagg atataataat
    23761 tttaatacct cattgtattc aagagaatag ttgtaaatta aaagttacta atgatataga
    23821 taattgtaaa gaatgtggaa gatgcaacat aggagaatta attaagctaa aggaaaaaac
    23881 taatgtaaaa atatttgtag ctacaggagg aactctagct agaaaaatta taatggacac
    23941 taaacctaaa gctgttgtag ctgttgcatg tgaaagagat ttgacatctg gaatacaaga
    24001 tattaagaaa attcctgttt taggtgtatt taacaaaaga cctaatggac cttgtgtaaa
    24061 tactaatgta gacatgatgg atattgaaaa agctataggc tttttaacag gaaaaaatat
    24121 attagtttgt aattaaactg tttttaagtt aagtgaatat taagaggatg tataataaaa
    24181 atatatttta ggagagtgtt atttatgtac cctgtatatg gcggtttttg gggatttgac
    24241 ccaacaatgg ttgtattaat tccagctata ttacttacta tatatgcaca atttaaagtg
    24301 agttcaacaa caaataagta tttaagagta aatacacgtc gaggatatac tggagagcaa
    24361 acagcaagaa gagtacttga ttcaaatggt ttgtatgatg taaagataga aatggttaga
    24421 gggcatctaa gtgaccatta tgacccaaga agaaaggctg taaggttgtc agaagatgta
    24481 tattatggaa catctataac ttctgttgca gtagctgcac atgaatgtgg tcatgccata
    24541 caacatgcta aaggttatgc acctcttcaa ataagaagta gtttagtacc agtagtgaat
    24601 tttgcttcaa gtatatcttg gtttttaata tttttaggat ttattatggc tggtccattt
    24661 ttaaaaattg gtatactatt gttttcggct tcagtgttat tccaaataat aacattgcct
    24721 gtagaattta atgcttcaag tagagcaatc gttcaacttg ggaatttagg tattatagat
    24781 gaaagtgagt caagacaaag tagaagagta ctttcagcag cagctttaac atatgttgca
    24841 gcagcattgg tttcaatact tcaattactt agattgttac taatagctca aagaagaaat
    24901 gactaaaagt aaagtgggtt gtttagaaac aacccttttt tatgtttaat aaaagggaga
    24961 aatttatatg gatgcaagag aagttggatt taaagtatta tgtgatatag aaaaaaatga
    25021 caattattca aatattgcaa taaataagca ttttaaaaat ttagaaataa gtgatatgga
    25081 tagaggactt gcaacagagt taatttatgg agtaatagaa aataaatatt atttagatta
    25141 tataatcaat aaactttcaa aaataaaggt aaaaaaaatg tcaacttatg ttaaaatctt
    25201 tttaagaatg ggaacatatc agattttatt tttagatagt atatcagatt atgcagcagt
    25261 taatgaaact gtaaaattat ctaagaaata tgataaaaaa tcttctggtt tcataaatgc
    25321 tattcttaga aatgaaataa gagctaaaga gactataatg gacataactg aggaagatag
    25381 tgtaaaatac ttatctataa agtattccta taactcttgg attataaaaa actggataga
    25441 taaatttgga caagaatttg ctgaagattt gttggaagca aataatgaaa agcctagtat
    25501 ctatataaga acgaatacac ttaaaataag tagagaagaa cttattgaaa aattaagtga
    25561 aatgggtatt gtatgtttaa aagttcctat ggtagaagaa gctattaaag tcgaaaaaat
    25621 gaaaaatata gaaaaaaatg aattatttaa ggctggttta tttacaattc aagatataag
    25681 ttctatgata gtagggaaag taattaatcc aaaagaaggc tcatttgttt tggatgtatg
    25741 tagtgctcct ggagggaaat ctactcactt agctacttta atgaacaata ctggtcaagt
    25801 agtagctaga gatatttttg agcataaact aaagcttata aactctactg taaatagatt
    25861 ggggcttaaa aatgtgtttg tagaaagatt tgatgcttct gagattgatg aaaatagtat
    25921 aagtaaattt gattatgttt tagcagatgt tccttgctct ggttttggta taataagacg
    25981 taaaccagaa attaagtata agaaagaaga agagttaaaa gacatcactt ctatacaaaa
    26041 gaaaatttta gaaaatgcat ctaaatatgt caaagttggt ggaactttag tatatagtac
    26101 atgtacaatt caagatgtgg agaatataaa tatagttact tcttttgttg aagaaaatca
    26161 caactttgaa tttgttccaa ttgatacagt aaatgtagac ttagacaatc aagataaagg
    26221 atatttaaag atatatccta atattcatgg tatagatggt tttttcatag caaaattaaa
    26281 gagaataagg tagtgagaga atggaaaaga aaaagatagt attaaaaaat tttactgaag
    26341 atgaacttaa agaatttatg aaaactatag atgaaaagcc ctttagagga agtcaaatat
    26401 tctcttggat atataagggt gcaaaaactt ttgatgatat gaacaatata ccaaaaagtt
    26461 tgagaaataa attagaagag gtttcatgta tagggcatat agatatagaa ttaaagttag
    26521 aatctaaggt agataataca aaaaaatatt tgtttttatt agatgatgga aatataatag
    26581 aaactgtgat gatggattat gatagtagag ttactgtctg tgtttctaac caagtaggtt
    26641 gtaggatggg atgtaatttt tgtgcatcta caatggatgg attaattaga aacttagagc
    26701 catgggaaat tttagaccaa gttattaaaa ttcaagaaga tactggtaag agggtatcaa
    26761 atcttgtatt gatggggagt ggtgagccac ttgataactt tgaaaataca aaacaatttt
    26821 taaaaataat taacgaaaaa aatggtctta atatagggta taggcatata actctttcaa
    26881 cttgtggaat agtacctaaa atgtatgagt tggcagactt agaaatagct ataaatttag
    26941 ctttatcact tcattcacca tatgatgaag aacgaagaaa aattatgcct gtagcaaatg
    27001 cttattctat tgaagaaata ttgaatgcat gtagatatta tataaaaaag acaaatagaa
    27061 gggttacatt tgaatattct cttataaaag gggtaaatga ctcagaaaaa gaagcaaagg
    27121 cattagctaa gctcctaaaa ggtatgctat gtcatgtaaa tttaattcca ataaataaag
    27181 ttgaagaaag agaatatgaa aagccagata aagcatttat ttataaattt agggatagtt
    27241 tggagaagaa caatatacct gccacagtta ggatgtctat gggttctgat attagtggag
    27301 cttgtgggca attgagaaga aaatacaagt aatttgtaag gaggaagtcg tatggtttat
    27361 agttgtgcct cccatatagg aaaaataaga aaaaacaatg aagactattg tgagggagaa
    27421 gttatagata cagaacatgg tccaatagga atatttgcca tagctgatgg aatgggtgga
    27481 cataaaaaag gtgaagtcgc cagtaaatta gcagtagaaa atataattga ttttctaaag
    27541 gaaaacttat tacaacatga taatgtaaaa atagactata tagatgatat attaaaacag
    27601 gcttataata atgtaaactc aattgtgcac aaaaaatcta tggaagatat agaatttgaa
    27661 ggaatgggaa ctacattagt tacggctata gtgtataata atgtcttata tgtggcaaat
    27721 gttggagata gcagatgtta tttgttaaca gatgaaaaat ttgataaaat aactatagac
    27781 cattccgtgg ttgaagaact tatgatggcc catgtcatta cagaagaaga ggcaagaaga
    27841 catccacaga ggaatagaat aactagagca attggaacag atgatatggt tgttgtagat
    27901 atttttaaga aagaaatcaa aaaaagtgat ataatacttc ttgcgacaga tggattaact
    27961 gggtttattg atgatgaaga tataagagat ttaatattag attatgaata tgagagaact
    28021 agtaatataa gtgaagaatt gataagtatg gccaatgatg tttctggtaa agataatgtt
    28081 tcggttatag tgataaaagt ataattagga cggtgatatt gtgggagata caattttagg
    28141 aaatcgatat gaaatcatca ggaagattgg tgatggagga atggcatttg tatatgaggc
    28201 taaagacaga ttattaaaca ggactgttgc acttaaagta cttagacctg aatttgtaga
    28261 tgatgatgaa tttctaacaa agtttaaaag agaggcagaa gcagtagcaa gtctttcaca
    28321 tccaaatata gtaaatgttt atgatgttgg tgaagatgga aaagttcact acattgtaat
    28381 ggagtttgta gatgggaaga atctaaaaga gattatacaa gatgaaggta tattagatga
    28441 atatactgca ttagatataa caaaacaaat agctatggca cttagtgcag cgcacaaaaa
    28501 ggggattata catagagata taaaacctca taatattctc atatcaaatg aaggaagagt
    28561 agtaaaagtg gctgattttg gtatagccaa agctgtttca aattcaacaa tgactaatat
    28621 tggtagtata ataggttcag tgcattattt ttcaccagag caagctaaag gtaaatttgt
    28681 aactaataat gctgacctgt attctctagg tatagtttta tatgaaatgt tgataggaaa
    28741 agttccattt aggggagata gtccaatttc tatagcactt caacacatta atgatgatat
    28801 agactttaca tcagaagaaa aagttaggat tcctcaaagt gtaagaacta caataaaaaa
    28861 gcttacagaa aaatctagtg cagatagata tcaaactgct gaagaattga tagaagatat
    28921 agagtatata gaaaaaaata ttgatttaga ttttataaaa gaatatgatg attttgcaac
    28981 taagaaaatt gatgaaaaag aaattaataa agttgtaaat ccaacacttg caaaaccagc
    29041 tcctgaaaaa gtggttaagc ctgttgaagt agctgatttg gatgatgatg aagattacta
    29101 tgatgatttt tatgaagaag atgatgatga agatgaagaa gaggaagaga taatgagagc
    29161 taaaaagaat cagagaccta agagtactcc aagtaagaga actaagaaaa agaagaaaaa
    29221 acaggaaagc ccaaaatcta gaagaagatt aaaagtaatt gcggctgttt tgatactgat
    29281 attgtgtgct caagtattct tagcatataa gtttttattt gctggagggt ttggcaataa
    29341 atctttgact gttcctaatc ttgtaaatat gacattagaa gaagctcaaa gtgctgttga
    29401 aaaagaagga ttatacctga gtgtaaaaag tgaagagtat aatagtgaag ttgatgaaaa
    29461 ttgtataata tcacagacac ctgaaggtgg aagtacaaat gtcaaaaagg gtgatactat
    29521 aaatgttgtc gtaagtaaag gttcaagtca ggcatctgtt cctaatgtag tgggacttac
    29581 tttgagtaat gcaaaacaac ttattgaaga aaataactta aaagtgggaa ctgttaagta
    29641 tgaatacagc tcaatatata aagaaggtac agttttaagt cagagtccag gtgctggctc
    29701 atcaagagct caggaaggtg atgaggtaaa tctatatgta agcaaaggtt cagaaaaatc
    29761 aaatacacag actccaacag taccagataa aaaaccaaca acaccagaga ataatacacc
    29821 aacagaacct ggttcaaact caggaaacag tggtggaaat tcaggaggct caaattcagg
    29881 aaatagtggt ggaaactcag gcaataatgg aagtaactca ggaggttcaa actcaggaaa
    29941 tagtggtgga aactcaggag gctcaaactc aggagataat ggaggaagtt caggaggctc
    30001 aaactcagga gacaatggag gaagttcagg aggttcaaac tcaggagaca atggagatag
    30061 ttcaggaggc tcaaactcag gagataatgg aggaacttca tcaggagctg gcgcaaacat
    30121 tggtaaaaca gaataaatat taataaaata agagctgtcc ttttatagaa aatattttct
    30181 ttaaagggca gtttgttttg tataaaatac tgaattggtt ataatataag gagaaaagta
    30241 tttatgaaga agaaaacagt tttaattaca ggaggagcta gagggattgg taaagctatg
    30301 tctaaagctt ttgcaaaaga aggttacaat gtattagtaa attttaataa gtcagaaaat
    30361 gaagctaaag agttgtacac tatattaaat gaaaaaaatt tttctgtaaa actttttaag
    30421 gctaatattt caaatagaga agatgtagag gatatggtag attattgtat aaaagaattt
    30481 ggaggattgg atgtacttgt aaacaatgct ggtgtaagtc aagataagtt gtttacagat
    30541 ataactgatg aagattggga taatatgatg aatattaatt taaaaggaag cttttattgt
    30601 tcacaagttg ctttaaaata catgatatca gaaaaaaaag gaaatataat taatatatct
    30661 tcaatctggg gtatatcagg tgcgtcttgt gaagtgcatt attctataac aaaagcaggt
    30721 ataataggta tgacaaaggc attagctaaa gaagtaggac catcaaatat tagagtaaat
    30781 agcatagctc caggtgtcat taatactgac atgttatctg gatataatga ggaagatata
    30841 gacgctcttg tggaagaaac ccctctaatg aggctaggaa ctccagaaga tatagcaaat
    30901 tgtgctattt tcttggcatc agacaaatca aattttatca ctggtcaggt aattagtcca
    30961 aacggaggat ttgttattta aaaaaaatgt ggtattatat aaaaatagga aagaataaca
    31021 cgatatacta ctcataaaca gtatgtgttt atataaattt tataaaggga ggcatttgat
    31081 gctagaagga aaaattataa aggggattag tggattttat tatgtagata cttataatgg
    31141 tatttatgaa tgtaaggcta gagggatatt tagaaagcag aaaataacac ctttggttgg
    31201 tgatagagtc aaaataagca ttgttgatga agatgaaaaa aagggtatct tagaagaaat
    31261 cgacagtaga gatacagaac ttataagacc tcctattgca aatgtagata aagcattaat
    31321 agtattcgca ataaaaaatc ctaaaccaaa cttatctctt ttagatagat ttatagtttt
    31381 ggcagaaaaa gaaaatttag aaactgtaat aatccttaca aaagcagatt tagatgataa
    31441 tgatacatta gaaactgtta aaaatatata tgaacttagt ggttataaag tcattcctgt
    31501 aagcaatatt actaagttaa atatagataa agtaaaagaa gaattaaaag aaaatgtagt
    31561 agtgtttgca ggaccatctg gagttggaaa atcaagctta ttaaatgaaa tagatgaaaa
    31621 ttttaaactt caaacaggag ttgtaagtga taaaataaaa aggggaaaac atactactcg
    31681 tcacgctgaa cttttaaaat tagaatttgg tggaatggtt gcagatacac caggttttag
    31741 ttcccttgca cttgaagata tagaggaagt tgaacttaaa gattatttta tagaatttga
    31801 taaatttaat gattgtaagt ttggttcaaa gtgtattcat gaaaatgaac ctaattgtgc
    31861 tataaaagaa gctgttacaa atggagaaat atctaaagaa agatatgata gttatataca
    31921 gctgctacat gaaataagac aaaataatag taggaggtat tagaaacatg attaaattag
    31981 caccatcaat attatcagca gattttgcaa aattattaga agatgtaaga aaagtagaaa
    32041 gtgcaggatg tgaatatctt catatcgatg taatggatgg acactttgta ccaaacatta
    32101 cattaggacc acttgtagtc aaaagtttaa agaaagaaaa tataaatatg gtatttgatg
    32161 ctcatcttat gatagaaaat ccagaccagt atattgaaga atttgtaaaa gcaggatgtg
    32221 atataataac tgttcaccag gaggcttgtg ttcatctaca tagaactata caaaatataa
    32281 aatcacatgg aataaaagct ggtgtagtac ttaatccagc tacaccagta gatacgatta
    32341 aacatgtact atctgattta gatatggtat tattaatgtc tgttaaccct ggatttggag
    32401 gacaatcatt tattccttgt gttttagata agattaaaga gttaaaagct attatagata
    32461 gtcaaggatt aaacattgat atagaagttg atggtggtat aagccctaaa aacgtagccg
    32521 aagttgtaca agcaggagct aatgtaatag tagcaggttc tgcaatattt ggaagtgatg
    32581 acattcaaga aacagttaat ttatttagaa agaatgcttc tcttgaagaa ttagtttaaa
    32641 tttaataatg aaaatctgta ttgttttaaa tggcgaaatt gaagattacg aagttacaag
    32701 agatattata attaaggaat gttatgactg cataatatgt gcagatggag gggcaaatca
    32761 cacttataaa atggaaataa tgccagatta tatattagga gatttagatt cagtagaaga
    32821 agaaaagata aacttctata aaaataaagg tgttaaattt gaaaaatttc catctaaaaa
    32881 agatgagact gatacagagc tttgtctttt tttagctaag acacttaaag caaatcatat
    32941 tgattttttt ggtgctttag ggggaaggat tgaccacact cttgcaaata taaagctatt
    33001 atattattta aaagaagatg gtatatattc taggatactt tctgataaag aagagatgta
    33061 tatagttgaa aatgaggaaa tttcattgta tggaaaccca ggggacacta tttctgtaat
    33121 agctataaat ggtgatgcta agggtgtaac acttactggc ttagaatacc ctctcgataa
    33181 ttattatatg aaatactctg tacctattgg aatatcaaat gttatgttga gtaattcttg
    33241 taagattaaa gtagagcaag gctgtgtact tgtagttaga aatttataaa atttgatttt
    33301 gacaattggt tttatattgt agagcagata caatcaagta aaatatattt atgattgtat
    33361 ctgcttttta gtatataatt ataaatatat aataaacttt gacataaggg ggattttttg
    33421 tggtagatag tattattttt gatttagatg gaacattgtg ggattcaact gaagaagtat
    33481 gcaaagtctg gcaggatgtt ttaagtgagc ataaggaaat agaattaagt gtcacaaaag
    33541 atttatttag aagtcttatg gggctttctt ttgaggaaat agcaaatcgt ttatttccaa
    33601 atttaagtga agaagaaaga atgaatttat tgaatgaatg ttctttgaaa gaatgtgact
    33661 atttatctga aaatggagct aaattatttg atgatataga agatactctt aaattactat
    33721 ctaaaaaata taaattattt atagtaagta attgtcaagc tggatatata gaatcttttt
    33781 taaaagcaca taaactagaa cagtatttta tagattttga atgtcctggt aatactggtt
    33841 tgcataaagg agaaaataat aaacttataa ttgaaagaaa taaattaata aatccaatct
    33901 atgttggtga tacgcaaggt gatgcaaata gtgctaagtt tgtaggtata ccttttatat
    33961 atgccaaata tgggtttggg aatgtagatg tatatgatta ttttatagat tcatttaaag
    34021 atttgttaga acatgatata ttaaaataag atgagtttaa aaattatatt aaaataataa
    34081 aaaagttatg gggggattta aaaatggaat tacaagatac tatttttaaa agacaaagtg
    34141 taagaaagtt taaaaatcaa gatgtatcag atgaagatat tttaaaaatg ataaaagcag
    34201 caggtgcagc accatcaggc aagaatattc aaaactggca ttttgttgtt ataaaacgcc
    34261 gtgatttaat ggaaaagata gcagatgtga taactaaaaa gcaacaagaa atacttgtag
    34321 aaatggataa agtatcagtg gataaggcta atagatttag aaaatttgtt aaaaatttta
    34381 cactatttta cttaaaagct ccagttttag tattagtttt tacaaaggta tataatccat
    34441 caggatatta tgaattagag cttatagatg cacctaagga gactatagat aaattattta
    34501 taagaaatcc aggtatgcag agtctaggtg ctgcaattga aaactttaca ctatcagcaa
    34561 ttgaacttgg atatggttct tgttggttga caagtcaaaa ctatgcagca gatgagatag
    34621 aagctgtttt agaagcagaa actggttttg aaaagggaga gtatttttta ggtgcaatgt
    34681 tagctcttgg agtgccagag gacaatttga agagtccatc taaaaaacca gtagaagaaa
    34741 tatgtacatt tataaaataa aagtcaagga cattttaaaa tcgaagtaaa ttaaaaacaa
    34801 aataaattaa aaataaatat ttaaaaaatt aaaaagtagg ttttattgat attaatttta
    34861 tatttcaaat cattaattta attaatacaa taagaactac ttttttttgt atattaattt
    34921 taaatatatt taaatcttta gaaaatcttt agaaaagtat atgtaatatc ttaagttttt
    34981 aattttaata ttaagtatat aaaacaaaat gaaaaaatat taaatttaaa ctagaaggag
    35041 gtttctaaga tggacattaa atacaaataa ccagaacaaa agatataaat atatatataa
    35101 atggaaaaac ttctatcata ttgtataatc atatatgtaa agaaaattta taaattaata
    35161 taaaggagat tagaatgaat tcatataata ttttggtggt agaagatgaa aaggagatag
    35221 cagatgctat agaaatatat ctattaaatc aaggatataa tgtttttaag ggatacaatg
    35281 gattagaagg tcttaaggtt atagaaaacc aagaaataca tttagctata gttgatataa
    35341 tgatgcctaa aatggatggt ataacactta caatgaagct tagagaaaac tataattttc
    35401 cagtaataat gctatctgca aaatcagaag aagttgacaa aataatgggg cttaatatag
    35461 gagcagacga ttatgtaaca aaacctttta agccattaga actattggct agagttaatt
    35521 cacaacttag aaggtacact aagtatctaa acatggttgg aaataaagag caaaaggtag
    35581 aaagtgacga tagtgtgttt gcaataggcg gattagaatt aaatgaaaat acaaaagaag
    35641 ttagtattga tggaaagtat ataaaaacaa ctcctataga atttaaaata ctaagtttac
    35701 ttatgagaaa tgcaggaaga gttttttctg ctgatgaaat atatgaaaga gtttggaatg
    35761 agaatgctgt aaatacagat acagttatgg tacatgtgag gaatataagg gaaaagatag
    35821 aaattgaccc aaaaaatcct aaatatttaa aggtggtgtg gggtgttgga tacaaaattg
    35881 aaaaaattta aagataaata tatagttact attatagttt ttatacttat gttatcagca
    35941 tcattaggta tgattagtca atattctact atacaagcaa gtgctaaagg tggagcagag
    36001 aatccatttg aacaggaaag ctttgtagat agtatatata aaggaagtta tgttttatat
    36061 cataatatga aggaagaaca agaaggtaag atgatacaac catcaaagct atttatcaat
    36121 gaagaaacta tgaactatat aaaaaatgta agtaaagaaa aagatactta tgatggttca
    36181 gaaacttatt atacagatga tagtattaat gcagatttta atgaaaactt tgaaaattgg
    36241 gagcatttca ttaaagcgtc aggaaagaat ttaaagtact atttagttga taaagaaagc
    36301 aattttgaat tttttaatga taataaagaa ttaggagtac ttgatacaac aaaaaatgat
    36361 gcttcaaaag aaaattctaa tgaaaatcag tcaaaaaata cagaaatgaa tgatgaggaa
    36421 agacaaaagc agattacaga tataaaagaa aactatagat tctacgttgt aatgaatttt
    36481 gataaagatg gaaaagtaaa tattgtggat tcttatggag taaatcaaaa aattattaat
    36541 caaatgttgt tatctaaagc aagagaagat ttaatggata caagtttagg aggaaattca
    36601 atttcatata agttatctcc aataaaaaat ataacattag tatatgctgt acctaaagta
    36661 ataagtaaag gtgatagtgt agagtatggt tatggaaata cttattttga tgatggtata
    36721 atttcatata taagcaatgt agaaaataat tcttattata agatttctgg aatgatttta
    36781 aaaattatat tagctatagt tgttattgta gctttagcat tcccatatag aaaatctaaa
    36841 gagttgctag gatttgatac attatcaaga ataccgttag agatactctt tattgctctt
    36901 gctattatac aggggcttgt agatttatat cctactgtta taataagtaa taccttgagt
    36961 gggaattatg tagagtacct tataaacaat gacataagtg ctaaaatggc tagtatattg
    37021 gtaagtttaa tgaatttaac ttattggttt gtaatattct caataatatt tgtatttata
    37081 atacttttaa aacacatttt aaatgttgga ataagagagt attttacaaa acatactgtg
    37141 acaggaatgt tttttgtatg gttaaaaaaa gcaggaaaag cagttattaa tcaaattaga
    37201 acaataaatc tgaaagaaaa gccaaataaa actatagtta caattttggg aataaattta
    37261 ttgattatag ttataatgtg tagtatgtgg ttctttggaa taatactcgc attaatatat
    37321 ttagtagtat tatttaagat actgtcagat tactctaaaa agacaatata tgaatataat
    37381 caacttttag atgttacaaa gaaaatttca gatggaaact tagatgtaaa tatggaaaaa
    37441 gatttaggat tttttaatcc tataaaagat gaattaggaa atattcaaac tggatttaaa
    37501 aaagctgttg acgaagaggt taagagtcaa aagatgaaaa cagagcttat atcaaatgta
    37561 tcacatgact taaaaacacc tctaacatct ataatcactt atatagattt attaaaagat
    37621 gaaagtataa cagatgaaaa tcgtaagttg tatattgata cattagacag aaagtcacaa
    37681 agattgcaac atctaataga agatttgttt gaagtaagca aggcaaatag tggagatgtt
    37741 tatttgaata tagtaaatgt ggatattgta tctctaatga agcagacact tttagaatta
    37801 gatgacaaac ttgcagattc atctctaata gtaaagaata acttttctaa tgaaaaaata
    37861 atattaccat tagatagtca gcgtacattt agagtatttg aaaatcttat aataaatata
    37921 agcaaatatg caatgccaaa ttcaagagtg tatattgata ttttagaaac agataataaa
    37981 gtaaatatta tgcttaaaaa tatgtctgca tctgagattg attttagtgt agatgatata
    38041 atggagagat ttgtaagagg agataaatca cgtaacacag aaggttctgg tcttggttta
    38101 gcgatagcaa aaagttttat agaattacag ggtggtaaga tgaatataag tgtggatggt
    38161 gatttattca aagttacaat tacttttaac aaaaattaga ttagacttct ttaataatta
    38221 taaagtctat agaataaaag aatctggaaa tatttatgtg tattctttaa ataaaaattt
    38281 at
//
LOCUS       DAMJVI010000283        35395 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.032, whole genome shotgun sequence.
ACCESSION   DAMJVI010000283 DAMJVI010000000
VERSION     DAMJVI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35395
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.032"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            227..844
                     /locus_tag="PC545_003847"
     CDS             227..844
                     /locus_tag="PC545_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDF2458510.1"
                     /translation="MCKKMYLLVVGILIAGLLTACNGSPTKDSEQAQDNTKKEANVSN
                     NALKDEKNNENLMEQDFKVPYTDAINIFKDKYKDADIVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTEKLDSEDLNGVARKEKLDFNDIMTPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKVDSKKGTVLKVEKED"
     gene            952..3483
                     /locus_tag="PC545_003848"
     CDS             952..3483
                     /locus_tag="PC545_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDF2458511.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDEDEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSNYKVNLFDVIEHKQEDILAKIAVSQGVLFGTPTILGDALKPIWDILISL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMTAVKPFAVNFKPSNEEIDKLHSY
                     TGKFLDKLNSTFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEVEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKDWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVV
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVVFKDGDSVDC
                     DMVVFSIGVRANTQMVQDTSVKIDRGIVVDKTLQTNVKDIYACGDVAQVGNISLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDGSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            3917..4618
                     /locus_tag="PC545_003849"
     CDS             3917..4618
                     /locus_tag="PC545_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanG-Cd-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDF2458512.1"
                     /translation="MNENILIVDDEKEIADLIEVYLKNEGFTVYKFYTGKEALECIES
                     KNLDMAILDIMLPDIDGFHICQKIREKYYYPIIMLTAKIEDIDKIMGLTIGADDYITK
                     PFNPLEVVARVKTQLRRYMRYNNSYEQQSIIVNEYDIKGLIINKETHKCSLFGKEVAL
                     TPIEFSILWYLCEHQGKVVPSEELFEAVWGEKYLDNNNTVMAHIGRLREKIHEPSKNP
                     KFIKTVWGVGYTIEK"
     gene            4668..5750
                     /locus_tag="PC545_003850"
     CDS             4668..5750
                     /locus_tag="PC545_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDF2458513.1"
                     /translation="MRTALMAFIAIIFIWILYSFILYGKFSIWIVSFFENAFRMEYKA
                     ALNLYQQIFRNSMELFMILAMVIVFFIIFRVYLNWFTKYFSEINQGIDSLIKEDVGEV
                     ALSPELLAIEKKINSIKHILEQRKFETQMAEQRKNELIVYLAHDLKTPLTSVIGYLTL
                     LRDESQISEELRKKYLSISLDKAERLEDLINEFFEITRFNLSNISLEYSTVSLTRMLE
                     QLTYEFKPTLMSKNLKYTLNVTPNMMIRCDVNKMQRVFDNLLRNAVYYSFEDTTIEIT
                     AVQEDDYIKLKFINHSNTIPEEKLERIFEQFYRLDTSRNSNSGRAGLGLAIAKEIVEL
                     HNGTITAHSENNIITFKVIIPIVGNL"
     gene            6011..7111
                     /gene="vanG"
                     /locus_tag="PC545_003851"
     CDS             6011..7111
                     /gene="vanG"
                     /locus_tag="PC545_003851"
                     /EC_number="6.3.2.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-serine ligase VanG"
                     /protein_id="HDF2458514.1"
                     /translation="MNNKKIAVIFGGNSTEYDVSLQSAFAVLENLNTEKYDIIPIGIT
                     REGNWYHYIGDYNNIQNNTWFERDTDIIPVIVSQNRVKNGIIEFLIPDAVSQNHIPNS
                     AIESCENDIKYTKLDFVFPVLHGKNGEDGTVQGLFELAGIQVIGCGTLSSALCMDKDK
                     SHKLVHSEGIKVPKSILLKYSDKFNVAELSKDLTYPLFIKPVRAGSSFGISKIYKKEE
                     LQVAVDLAFEHDNEVIIEENIDGFEVGCAVLGNNDLLVGRVDEIELSKGFFDYTEKYT
                     LKSSKIHMPARIDLDCEKRIQETAKIIYRTLGCSGFARVDMFLTPSGEIIFNEVNTIP
                     GFTSHSRYPNMMKGIGLSFEEILDKIIEVYIK"
     gene            7108..7914
                     /locus_tag="PC545_003852"
     CDS             7108..7914
                     /locus_tag="PC545_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDF2458515.1"
                     /translation="MMNNILLDKNKEDKMTNTILLTKDKVYDGNLILVNAFLPVKTSE
                     DIDLIPVDTRFPSILMKREATNILQNILKSICGINEIVPVSGYRTAEEQQDIYSSSLR
                     DNGKDFTKKFVALPNHSEHQTGLAIDLGLKKEVIDFICPEFPYNGICNEFRKAALHYG
                     FIERYQCKKEEITGISQEPWHFRYVGYPHSEIINKMNLALEEYIEYIKRFSYTNCLKI
                     EDKEKIIEIFYVPIHTKEEVRISIPEQCVYQISGNNKDGFIVTFWRNRKD"
     gene            7932..10070
                     /gene="vanT"
                     /locus_tag="PC545_003853"
     CDS             7932..10070
                     /gene="vanT"
                     /locus_tag="PC545_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021418922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine racemase VanT catalytic subunit"
                     /protein_id="HDF2458516.1"
                     /translation="MIRNKSYAGLDYFRIIAALLIIAIHTSPLLSYSKMADFVLTSII
                     ARIAVPFFFMLSGFFLISTYNYNCNKLKSFLKNVLFIYGIAIVIYIPLNIYTGYFSMN
                     NLVPNIIKDIVFDGTLYHLWYLPASIIGASIAYFLVKKLGLKYSLVITIVIYVIGMFG
                     DSYYGFLEKIPLLKNLYGYIFEVSDYTRNGIFFAPVFFILGGIISNKSNYMSTKNSLI
                     GFLISFLLMLCEGMFLHKLGVQRHDSMYIMLLPCMYFLFTALTFLKGSRARLLRTSSL
                     IIYIIHPMMIVVIRMFSKIFGIQTVLVDNSLIHYIVVSIASFIASIIIILILKPLKKD
                     LKGEHKVNTNRAWIEIDLNNLEHNVKMIQKAMPEDCELMAVVKANAYGHSAFEVATCA
                     NQIGVKAFAVATIDEGIDLRRYGILGEILILGYTNPIRAKELHKYNLTQSVIDLNHAI
                     SLNKQKYNIKSHIKIDTGMHRLGFDVNDVRSILKAFKLKYLDICGIFTHLCVSDSLKD
                     EDVDFTNKQIECFYKLIDFLLKKGIKIPKVHIQSSYGFFNYPNLKCNYVRIGIALYGI
                     LSFPNDTTKLQLDLRPVLSLKSQVILIRKIQKDDSFGYGRVFIASRDSVIAILSIGYA
                     DGIPRNLSCGKSYVIINGCRAAIVGRICMDQLAVDITDIPNVEVGNTAIIIGRDNLSE
                     LSVSEVANNSCSISNELLSRVGRRLNVIKK"
     gene            10384..11004
                     /locus_tag="PC545_003854"
     CDS             10384..11004
                     /locus_tag="PC545_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDF2458517.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFIGCLLSWIGSGLGTTSLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDLKDFVPAMLAGKFVMFIVISYVASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHHNDKDDKMI"
     gene            complement(11170..11706)
                     /locus_tag="PC545_003855"
     CDS             complement(11170..11706)
                     /locus_tag="PC545_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDF2458518.1"
                     /translation="MIKETHERGGYIFALLILPFINNLYLVKYDIAYKIVLIIIYIYF
                     AYLGSLFPDIDMRGSYISKKFMLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKFISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(11819..12493)
                     /locus_tag="PC545_003856"
     CDS             complement(11819..12493)
                     /locus_tag="PC545_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDF2458519.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNAALEKYPELYNYSLCELLQNLDSLPKDIATTVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKSALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNVVNWNGALENYK
                     NLKSQD"
     gene            12696..14570
                     /gene="asnB"
                     /locus_tag="PC545_003857"
     CDS             12696..14570
                     /gene="asnB"
                     /locus_tag="PC545_003857"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDF2458520.1"
                     /translation="MDGNISIYYKGDISKKEDIKFKIKESVNNTRLSSRIEYKDEEKT
                     FDFERFNVIDAIYGNKPFEKDGKVIVFNGNIYNYHEIKEELIKKGHNFTTNDDIEVLL
                     ASYIEFGKNCVHKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKENAIIFSSEYK
                     FLLEYMKNLNINERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNK
                     LEFRSSTKFFYTKNHLGNRDVNEDDIRNIVVDSIVTHMEEEKEIGTFLSGGIDSSIIT
                     TVASQINPNIKSFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKSLPNVIY
                     SLDDPIADPSEVGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPT
                     YIKSILGRVSELMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIY
                     EYLLSNLYRDAQKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKD
                     VFDVAKNLRMEQKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGDI
                     VKETIYNSNVDEFIDKKYAIKLLDTHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY
                     "
     gene            complement(14662..16098)
                     /locus_tag="PC545_003858"
     CDS             complement(14662..16098)
                     /locus_tag="PC545_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDF2458521.1"
                     /translation="MICTIKPLKISIKKSTEPLIEFISDTTYKNKRSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPTIPYIRLNNLKINFDKEQTDKMLKIFNIYNSSK
                     FNSWDLYNIDFPCKIENDTLNWTKKIAFKNTLDLFSTSMPNCTSSIIKNFAVKLLCWI
                     DYFLPKLFYNKVKTTISPKIVYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSTLFELSRKTPNILEIPFERHITNINYSENTNKTSPTTNKPNSKNTLNNTNLT
                     SKKIVQDEEVELSYEDLAKLSASVVMIVVCNNENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSDYDLAVIKVDRKCKPIPVKVSKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESNLVKIFANNFIEIVN"
     gene            16427..17005
                     /locus_tag="PC545_003859"
     CDS             16427..17005
                     /locus_tag="PC545_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2458522.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            17044..17622
                     /locus_tag="PC545_003860"
     CDS             17044..17622
                     /locus_tag="PC545_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2458523.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLETMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            17708..18337
                     /locus_tag="PC545_003861"
     CDS             17708..18337
                     /locus_tag="PC545_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HDF2458524.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGTGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            18327..21086
                     /locus_tag="PC545_003862"
     CDS             18327..21086
                     /locus_tag="PC545_003862"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HDF2458525.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMISKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     INSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQVRKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKQE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            21173..22735
                     /locus_tag="PC545_003863"
     CDS             21173..22735
                     /locus_tag="PC545_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HDF2458526.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKSGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDRVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNIEFEEANIIEEKISFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFKFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGAIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIVFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            22736..23872
                     /locus_tag="PC545_003864"
     CDS             22736..23872
                     /locus_tag="PC545_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDF2458527.1"
                     /translation="MGININSFSKDKNSVEVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSTQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            23936..25012
                     /locus_tag="PC545_003865"
     CDS             23936..25012
                     /locus_tag="PC545_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HDF2458528.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNRHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETKEFIYNEKKELSFTNLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMTNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNETNPHFLWAK
                     KTLILSKIYGVLNKGVDYEELLKF"
     gene            24990..26228
                     /locus_tag="PC545_003866"
     CDS             24990..26228
                     /locus_tag="PC545_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HDF2458529.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDVGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLMATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYSITKLETISSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITMASKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQYDIYYHST
                     TRSPIINIDKNNYPIKSKFMLKSFYNIDIQNYIYNIDKHNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            26282..27370
                     /locus_tag="PC545_003867"
     CDS             26282..27370
                     /locus_tag="PC545_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HDF2458530.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYIRSKYNVSLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNVKFKKYFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLISKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFIL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDI"
     gene            27398..28189
                     /locus_tag="PC545_003868"
     CDS             27398..28189
                     /locus_tag="PC545_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HDF2458531.1"
                     /translation="MIVFSDLDRSIIYSNKFLNADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIHKFKDYLNVDGITNFKVAEDTFFYIVVDLSRFNLDSIKEYTNI
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAVGDSIMDYGMLNISNK
                     SYVLKHGDINKNEIENSFISSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(28304..28858)
                     /locus_tag="PC545_003869"
     CDS             complement(28304..28858)
                     /locus_tag="PC545_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDF2458532.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTHKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDEVCISVWAMTPPSF"
     gene            29054..30256
                     /locus_tag="PC545_003870"
     CDS             29054..30256
                     /locus_tag="PC545_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HDF2458533.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGITEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            30313..31293
                     /locus_tag="PC545_003871"
     CDS             30313..31293
                     /locus_tag="PC545_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDF2458534.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISENKINILIGLE
                     GLSHIGRNIDLLDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVNKME
                     KLGMIVDVSHLNEKSFWDLMSVASKPVIASHSNARSLCDAKRNLTDNQLKEISNTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NASVPSVDGLESTHTAQNIVVELENRGYKKKDIELISSGNFYRLFKDILK"
     gene            31541..32494
                     /locus_tag="PC545_003872"
     CDS             31541..32494
                     /locus_tag="PC545_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2458535.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEDINIMHIFAKNQHKLMIFIMS
                     RIPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFTFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            32484..33440
                     /locus_tag="PC545_003873"
     CDS             32484..33440
                     /locus_tag="PC545_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDF2458536.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFIIYGIDMNHLEYALSQRIPKI
                     FAMVLGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAMMIGASLLLYKTLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKAVKKMIILIAILVSISTALIGPMTFLGLLLANITREIFKTYKHTYLISGSMLIGM
                     ITLIIGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            33437..34192
                     /locus_tag="PC545_003874"
     CDS             33437..34192
                     /locus_tag="PC545_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDF2458537.1"
                     /translation="MIEIKNIFKRYKNKNVVDDVSFSIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRSLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVKKVG
                     RTDEIVNEKVLEDIYEMNFNIKNINGNRICIYF"
     gene            34284..35249
                     /locus_tag="PC545_003875"
     CDS             34284..35249
                     /locus_tag="PC545_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDF2458538.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKKNESKTSENSNNTMKITH
                     NLGETDVKLNPKKVVVFDYSALDTMDALGVAENLVGLPKASLPASLEKYKDDKYADLG
                     GLKEPDLEGIKSANPDLIIINGRQEDFYEQLSKIAPTISTSKDDKKYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFENKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDVKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
BASE COUNT        13794 a         3715 c         6124 g        11762 t
ORIGIN      
        1 ttcatgaggc actctttttg tatgaaatca aatatatttt tattatttca acaataaaaa
       61 agaggtctgt ttctattttg agacaatatt atttttatgt agatttaatc ttaatgagta
      121 taaagtttta aaactactaa aataggagga attttaatat aaaagggtaa ataaagggtt
      181 tagagggcat aatatagaat aaattaatta tttaaggagg gtttttatgt gcaaaaaaat
      241 gtatttatta gtggtaggta tacttatagc aggtttatta actgcatgta atgggagtcc
      301 aactaaggat tcagaacaag cacaagataa tacaaaaaag gaggcaaatg tatcaaataa
      361 cgctttaaaa gatgaaaaaa ataatgaaaa tttaatggaa caagatttta aagtgccata
      421 cacagatgca ataaacatat ttaaagacaa atacaaagat gctgatatag tagatttaag
      481 tcttgagaga gatttaaata aatttgttta tactgtggaa ggtgtagatg ataataatga
      541 atataagatg aaaattgatg ctaatacaaa agatgtatta gaagataaaa cagaaaagtt
      601 ggattcagaa gatttaaatg gagtcgctag aaaagaaaaa ttagatttta atgacataat
      661 gacacctcaa caagctatgg aaattgcatt aaaagaacaa aatggaatgg ttaaagagtg
      721 gagtttagat aaagatttag atgttacttt ttataaaata agaatagata aagataaaaa
      781 tgaatatgac ataaaagtag attctaaaaa gggaactgta ttgaaggtag aaaaagaaga
      841 ttagaagatt taaactatct ttaaaataat ttcttttttg aataaaattt gtttaaaata
      901 taacaagtag ggtataaaga gactaaataa ctattttagg aggagtacgg tatgtcattg
      961 aaatcgctaa aaataaaaga aaatttatat tgggtaggtt cgcttgaccc tgatttaaga
     1021 gtatttgaca taattatgta tacaccttat ggaactactt ataattcata tgtattaaaa
     1081 ggaacagaaa aaacagtttt atttgagaca gttaaagata agcattttga caattatatt
     1141 gaaagattaa atgatttaaa tatagatttt gaaaaaatag actatattgt tgtaagtcat
     1201 actgaaccag accatgcagg aagcgttgaa aagcttttgg atttagctaa aaatgcaaaa
     1261 gtagtagctt cagaaacagc aattaaatat cttaaggaaa tcgtaaacaa agactttgaa
     1321 tatgttgcag ttacagatgg agatacatta tcaattgggg acaaaacttt agaatttttc
     1381 tctgtaccta tgcttcattg gccagatacc atatacacat atataaaaga ggataaaact
     1441 cttgtaacat gtgattcatt tggtagtcac tatagtaatg ataagatagt aaatacactt
     1501 gatgaagatg aagaaaagga ttacttggat gctttgagat actattatga ttgtataatg
     1561 ggaccattta aaccatctat ggtaactgcc atagaaaaga taaaagattt agatatagat
     1621 actgtatgtc caggtcatgg tccagtatta actgaaaatc ctagaaaaat tatagacctt
     1681 tattacaact ggagtgtaaa tgaacaaata aagttagaaa aagaagttac aatttgttat
     1741 gtttctgcac atggatatac aaagattatg gcagaagcta taaaagcata tattgaaaaa
     1801 aatagcaact ataaagtaaa tttatttgat gtaatagagc ataagcaaga agatattttg
     1861 gcaaaaatag cagtatcaca aggtgtatta tttggaacac caacaatatt aggagatgcg
     1921 ttaaagccta tatgggatat attaatatcg ttaaacccag ttcttcatgg tggaaaagtt
     1981 gcttcagtat ttggttcata tggatggagt ggagaaggta tagaaaatgc tatggagaga
     2041 ataagtcagc ttagaatgac agcagtaaaa ccttttgctg ttaactttaa accatcaaat
     2101 gaagagattg ataaattaca ttcttataca ggtaaattcc tagataagtt aaattctact
     2161 tttggaagta aaaagaaaac taagaaattt aaatgtgtaa tttgtaatga agtttttgaa
     2221 ggtgatagtg ctcctagtgt atgccctgta tgtggagcta aggaagacca atttatagaa
     2281 gttgaagaag atgaagttac ttttagaaaa gatactgatg aatatttcgt aatagtagga
     2341 aatggagcag ctggttttta tgcagcagat gctataagaa aaagaaataa aacttgtaag
     2401 ataactatga tttctaatga agatgagttg acttactata gacctgcact ttctgatggt
     2461 ataaatgaag aacttggttc agatttctac atggaagata aagattggta tgataaaaat
     2521 aatatagtag taatattagg aacgaatgta gataaattag atgaagtgaa taaaactatt
     2581 atagtgaatg atggagctat taaatttgat aagcttgtaa tagctacagg aagtagaaac
     2641 tttataccac caattaaggg tcatgattta gaaaatgtgt ttactcttag aaatataaaa
     2701 gatttatata gtgtaaaaga agcactagaa aaatctaaga aagttgtagt gattggtggc
     2761 ggtttattgg gacttgaagc tgcatgggaa tttagactaa aaggattaga agtagtagta
     2821 gttgaagcca tggatagcat actatcaaaa caacttgata aagaaggcag taaaatatta
     2881 gaacaatgtg taagagatac aggaatagat gtaagattgg gtgtagcagt tgatggtatt
     2941 gaaggtgatg gaaaagctca aaaagttgta tttaaagatg gagatagtgt tgactgtgat
     3001 atggtagtat tttcaattgg tgtaagagca aacactcaaa tggttcagga tacatctgta
     3061 aaaattgaca gaggtattgt tgtagataag acattacaga caaatgttaa agatatatat
     3121 gcttgtggag atgttgcaca agtgggaaat attagtttag caatatggcc atcttcagta
     3181 gaaatgggaa aaatagcagg ggcaaatgca agtggagata atttgacatt tgaaagtgaa
     3241 gtatatccag tttcattaga tgcaatgaat gtaaaagtat ttagtattgg taatatacaa
     3301 aattttgata aagaaatttc atcaaaagat gaaggtcaaa gaatatataa aaaattattt
     3361 atgaaagatg gttcattagt tggagctata cttataaatg atttaagctg tactgttaaa
     3421 ttgataagat taatatctga aaaaggtgat tttgaggata ttatgaaggc tgatatacta
     3481 taatttaaat acattaaaat aaaaaggagt tgtcttaaaa ttgatttttt tctaatcaaa
     3541 ttttaaattc aactcctttt ttgaattttt atataaaata acatggaaat tttagtaatg
     3601 tctgtggtta catacatagg tttaattgtt ttttatatgg attgtctctg gtataataaa
     3661 ttgaattgtg tttgactgat agatattttt attgaaagtt aatacctgtt atgcgataat
     3721 aaggacgtgt taggggagtt tttataataa caattaatct cagtttttgt gttccaatag
     3781 tagcatcaac acattctgat gtagtagttc ttttcttata aaaattagtt acaaatatgc
     3841 ttgtaattat ttgatatgta aatttaccaa tattataaat gatgacctcg ataataagga
     3901 gagtgaaaaa taatctatga acgaaaatat tttgattgtg gatgatgaaa aagaaatagc
     3961 agatttaatt gaagtatatt taaaaaatga aggttttaca gtatacaagt tttatacagg
     4021 taaggaagct ctagaatgta ttgaatctaa aaatttggat atggctattt tagatattat
     4081 gcttcctgat attgatggat ttcatatttg ccagaaaatt cgagaaaaat actattatcc
     4141 tatcattatg ttaacagcta aaattgaaga tatagacaag ataatgggtt taaccatagg
     4201 tgctgatgat tatataacaa agccatttaa ccctttggaa gttgttgcaa gagtaaagac
     4261 acaattaaga agatatatgc gatataataa ttcttatgaa caacagtcga taattgttaa
     4321 tgagtatgat attaaaggat tgattatcaa taaagaaaca cataaatgta gtttatttgg
     4381 gaaagaagta gcattaacac cgattgaatt ttctattctt tggtatttat gtgaacatca
     4441 aggaaaagtg gttccttctg aagaattgtt tgaagctgta tggggagaaa aatatcttga
     4501 taataataac acagttatgg ctcatatagg gcgattacgt gaaaaaattc atgaaccttc
     4561 taaaaacccg aaatttataa aaactgtatg gggagtaggt tatacaattg aaaagtaaaa
     4621 atattaaagt caatcctgac tttactcaag tgagaaataa aatttttatg agaacagctt
     4681 taatggcatt tatagccatt attttcatat ggattttata ttcttttata ttgtatggta
     4741 agttttctat ctggatagta tcattctttg aaaatgcttt tcgtatggaa tataaagctg
     4801 cattgaattt atatcagcag attttccgta atagtatgga gctcttcatg atactagcta
     4861 tggtgattgt gttttttata atattccgtg tttatttaaa ttggtttaca aaatatttta
     4921 gtgaaataaa tcaaggtatt gactctctta tcaaagaaga tgttggagaa gtggctttat
     4981 ctccagagtt attggcaata gaaaagaaaa taaactcaat taaacatata cttgaacaac
     5041 gtaaatttga gacacaaatg gcagaacaac gtaagaatga acttattgtt tatcttgcac
     5101 atgatttaaa aacaccactt acatcagtta tagggtatct gacactactt cgagatgaaa
     5161 gtcagatttc tgaagaactg cgaaaaaaat atttatctat ttcgttggat aaggcagagc
     5221 gtcttgaaga tttaattaat gaattctttg aaattacaag gtttaattta tcaaatatat
     5281 cacttgaata cagtacagta agcttaactc gtatgcttga acaactaaca tatgagttta
     5341 aacctacttt gatgagtaag aatttaaaat atacactgaa tgttacacca aatatgatga
     5401 taaggtgtga tgttaataaa atgcaacgtg tatttgataa cttattacga aatgctgttt
     5461 attatagttt tgaagatact actattgaaa taacagctgt acaggaggat gattatataa
     5521 agcttaaatt tataaatcat agcaatacga ttccagagga aaaacttgag cgtatttttg
     5581 aacaatttta caggttggat acatctcgta attcaaacag tggtagagct gggttgggac
     5641 ttgctatagc taaagaaata gtggagcttc ataacgggac aataacagct catagcgaga
     5701 acaatataat tacctttaag gttattattc cgattgtagg aaatttgtaa gaatttaagt
     5761 ataaaaaaat aggaaattct ttaggataaa taaataaaga gcactcttgt tattgatata
     5821 attactgtat caaaacaaga gtgcttttat tttgatacag taattatatc aatagaaaaa
     5881 tgtgaaaata tattatgtgg aacagatgca attgtaagtg aaggttgatt ggatttgaaa
     5941 tagaaaaagt attttaacat gtttctgtag attgtataaa aataaataaa tcaacaaagg
     6001 agaatcaaag atgaataata aaaaaattgc agttatattt ggtggaaact caacagaata
     6061 tgatgtatca ttacaatcag cttttgcagt acttgagaat ttgaatacgg aaaaatatga
     6121 tattatacca attggaatta caagggaggg taattggtat cattatattg gagattataa
     6181 taatatccag aataacactt ggtttgaaag ggatactgat ataattccag ttattgtttc
     6241 gcagaaccgt gtcaaaaatg gtattattga atttttaatt cctgatgctg tttctcagaa
     6301 tcatattcca aatagtgcta ttgaatcatg tgaaaatgat attaaataca ctaaacttga
     6361 ttttgtgttt cctgtactgc atggaaaaaa tggagaagat ggaacagtgc aggggttatt
     6421 tgaattggca ggtatacagg ttattggatg tggtactctt tcttcagcat tatgtatgga
     6481 caaagacaaa tcacataaac ttgttcattc agaaggtatt aaagtaccaa aatcaatatt
     6541 actaaaatac tcagataaat ttaatgtggc tgaactgtca aaagacttaa cttatccatt
     6601 atttataaaa cctgtgcgtg caggttcatc atttggtatt agtaaaattt ataaaaaaga
     6661 agagttacaa gtagcagtag acttggcttt tgaacatgat aatgaagtaa taattgagga
     6721 aaatattgat ggttttgagg tagggtgtgc agttttagga aacaatgact tacttgttgg
     6781 aagagtagat gaaatagaat tgtcaaaagg attttttgat tatacagaaa aatatacatt
     6841 gaaaagttca aaaattcata tgccagctag aattgatttg gattgtgaga aaagaataca
     6901 agagacagca aaaattattt atagaactct tggatgttct ggatttgcta gagtagatat
     6961 gtttttaact ccatctggtg aaattatttt taatgaagtc aatacaattc ctggctttac
     7021 ttctcatagt cgttatccaa atatgatgaa aggtattgga ctgtcttttg aagaaatatt
     7081 ggacaagata attgaggtgt acataaaatg atgaataata ttttattaga taaaaataaa
     7141 gaagataaaa tgacaaatac tatcttattg actaaagata aagtttatga tgggaatcta
     7201 attctagtaa atgctttttt accagttaaa acatctgaag atatagattt aattcctgta
     7261 gatacaaggt ttccaagtat tcttatgaaa cgtgaagcta caaacatttt acaaaatatt
     7321 ttaaaaagta tatgtggaat aaatgaaata gtacctgtaa gtggatatcg tactgcagag
     7381 gaacaacaag atatatattc aagttctctg cgtgacaatg gaaaagactt tactaaaaag
     7441 tttgtagcac ttccaaatca cagtgaacat cagacagggc ttgctattga tttagggtta
     7501 aagaaagaag ttatagattt tatctgccct gaatttcctt ataatggtat ttgtaatgag
     7561 tttcgtaagg cagcactaca ttatggtttt atagagcgtt atcagtgtaa aaaagaagaa
     7621 ataacaggaa tatctcaaga accatggcat tttcgttatg tgggatatcc gcattctgag
     7681 attatcaata aaatgaatct cgctcttgaa gaatatatag aatatattaa gagattttcg
     7741 tatacaaatt gtttaaaaat agaagataaa gaaaaaatta tagaaatctt ttatgtgcct
     7801 attcatacta aagaagaagt gagaatctct atacctgaac aatgtgtata tcaaatatcg
     7861 ggtaacaata aggatggttt tattgtgaca ttttggagaa atagaaagga ctaagaaatg
     7921 gaggaacaag aatgattagg aataaatctt atgcagggct tgattatttt aggattatag
     7981 ctgcattact tataattgca attcatacat ctccattatt atcatatagt aaaatggctg
     8041 actttgtgtt aactagtata attgctcgta ttgctgtgcc attctttttt atgctatcag
     8101 gcttttttct aatttcaaca tataattata attgtaataa actgaaatct tttctaaaaa
     8161 atgtattatt tatttacggg atagctattg taatttatat tcccttaaat atttacactg
     8221 gatatttttc catgaataat ttagtgccca atattattaa ggacattgta tttgatggga
     8281 ctttatatca tttatggtat cttcctgcat caattattgg tgcaagtatt gcatactttc
     8341 ttgtaaaaaa actaggttta aagtactcac tagtaatcac tattgttatt tatgtaattg
     8401 gtatgtttgg tgatagttat tatggttttt tagaaaaaat accactatta aagaatttat
     8461 atgggtatat ttttgaagta tcagattata cacgaaatgg tatatttttt gcacctgtat
     8521 ttttcatact tggtggaata atatctaata aatctaatta tatgtcaact aaaaatagct
     8581 tgattggatt tcttatatca ttcttactta tgttatgtga gggaatgttt ttacataaac
     8641 taggggtaca gcgtcatgat agcatgtata ttatgttatt gccatgtatg tatttcttat
     8701 ttacagcctt aacatttttg aaaggttctc gagctaggtt attgcgaaca tcatcattaa
     8761 ttatttatat tatacatcct atgatgattg ttgttattcg tatgttttct aaaatattcg
     8821 gaatacaaac tgtcttggtt gacaatagcc ttattcatta tatagtggta agtatagcat
     8881 cttttatagc ttctataatt attatattga ttcttaaacc tctaaaaaaa gacttgaaag
     8941 gagaacataa ggtaaatacc aatagagctt ggattgaaat tgatttaaat aatttggaac
     9001 ataatgtaaa gatgatacaa aaagctatgc cagaagattg tgaattgatg gcagttgtaa
     9061 aagctaatgc ctatggtcat agtgcttttg aagtagcgac ttgtgcaaac caaataggtg
     9121 taaaagcatt tgcagtagca acaatagatg aggggattga tttacgtcga tatggaatac
     9181 taggagaaat attgattctt ggatatacta acccaataag ggcaaaggaa cttcataaat
     9241 acaatcttac acagagtgtt attgacctta atcatgctat ctctttaaac aaacagaaat
     9301 acaacataaa atcacacatt aaaattgata caggtatgca cagactgggt tttgatgtta
     9361 atgatgtgag gtcaatttta aaggcattta aattaaaata cttagatata tgtggcatat
     9421 ttacacactt gtgtgtttca gatagtctga aagatgaaga tgtagacttt acaaataaac
     9481 aaattgaatg tttttataag ttaattgatt ttctgttaaa aaaaggtata aaaattccta
     9541 aagtacatat tcaaagtagt tatggttttt ttaattatcc caatcttaag tgtaattatg
     9601 taagaattgg aattgcatta tacgggatat taagttttcc aaatgatacg actaaattgc
     9661 aacttgattt acgacctgtt ttatccttaa aatcacaagt aattcttatt agaaaaatac
     9721 aaaaagatga tagttttgga tatggaagag tctttattgc aagcagagat agcgtaattg
     9781 ctatattatc tattggctat gccgatggta tacctagaaa tttatcctgt ggaaaaagtt
     9841 acgtaataat aaatggttgt agagcagcaa tagtgggtcg aatttgcatg gaccaacttg
     9901 ctgttgatat tacagatata cctaatgtag aagttggtaa tacggcaata ataataggaa
     9961 gagataattt atctgaatta tctgtttctg aagttgcaaa taactcatgt agtatatcga
    10021 atgaattatt gagcagagta ggaagaaggt taaatgttat aaagaagtaa ctcaattaaa
    10081 tagtaatatt tgttgtactg tgtaatttat tttactctga ctagacattt ttagtaatat
    10141 tatgtctaat atatattttt tataacttac atgacaattt aagttataaa aaggtgatgt
    10201 ttcagatttt tattttgaaa catcaccttt tttgttataa tatataataa aataattata
    10261 ccttaactgt aaacttatat agattattta ctttttaata ttagattcat tttttgagat
    10321 tggtatataa taaacatata gagtatataa taatagttaa agttaaaaaa ggggaaaagc
    10381 aatatgcagt atatacaaga actacttgat ttgactcaaa attattgggt gttagccaca
    10441 ataatcggct tattaagtgc atttatagaa agctttatac cagccttacc acttgttgct
    10501 atagttacag caaatgctgc aatacaagga ttatttattg gatgtttgtt atcatggatt
    10561 ggctcaggac ttgggacaac ttcattattc ttattaataa gcagatttaa tgatagtaag
    10621 ctttttaata agttgagaaa tagtaaaact gaaaaagcaa taagttggat ggataaacag
    10681 ggatttaagt tattatttat agcgtatgcc tgtccattta tgcccggttg tttagttact
    10741 atcgcatcag cttttggtaa aaaagattta aaagattttg tgccagcaat gttggcagga
    10801 aaatttgtaa tgtttatagt tataagttac gtggcaagtg atatagaagg ttttataaca
    10861 agtcctttaa agatagcatc atttatatta ttggtgtttt tgtcatggaa gattggaaat
    10921 agagtaaata aaaatcttga aaatcataat tatgatttcc atcataaaaa acatcacaat
    10981 gataaagatg ataaaatgat ttagcttgta cgaaataaaa cgtatcttaa aatgatttag
    11041 tactaaatta ttttaagata cgttttattt tttgtattaa ggtacaaaat ttttagttct
    11101 tttgtaaact aaaatatata aaacttattt atacttagtt caaaatattg ttatttattt
    11161 tttctacaac tataataaaa tataaaattg atatccaatc aaaaaaataa ctatgaaatt
    11221 taatagctta gatatgaatt tttctgtttt tgagttagtt tttataggta gtaaagaaat
    11281 gtttatagta atgggataaa acaattcaat accttcttta gtaataaggt caagacacaa
    11341 gtgagataga tatcctatta taaacccact ggacaaacat gagaaaacta tattattgtt
    11401 agtgaagatt gtcagagatt taaaaaaact ataaataagt aataaggcaa ttagactgtg
    11461 agtaaaccct ctatgtctaa acctacttcc aaataatttg tatattaaca taaatttttt
    11521 acttatgtaa gaacctctca tatctatatc tggaaataaa gaacctaaat atgcaaaata
    11581 aatataaatt ataattaata ctattttata tgcaatatcg tatttaacta ggtacagatt
    11641 gtttataaag gggagtatca aaagtgcgaa tatataacct cccctttcat gtgtttcttt
    11701 tatcatataa aaatcctcct gaatcgtcac tataaacatt atattggtaa atttagacta
    11761 tcttttatta tattatatag caaacataag tttttttcaa aaggatttta atatttaatt
    11821 aatcttgaga ttttaaattt ttgtaatttt ctaaagcacc attccaattt actacattaa
    11881 accagttgtc aatgtattca tttcttctat tttgatattt taaatagtaa gcatgctccc
    11941 aaacatcaag tcctattata ggagttaggt ttttacttac agggctatcc tgatttggag
    12001 tagtcataat agataatttc ccatctttag tagctacaag ccaagcccaa ccagaaccaa
    12061 agacatctaa agcagatttt tggaactctt gcttaaattt ttcaaaagaa ccaaagtctc
    12121 tatcaatagc ttcttttaaa gattcagaag gtatggtttt ttctggcgtc attatatcaa
    12181 aaaagaattt atgattataa gctccacctg cattatttct tacagttgta gcaatatctt
    12241 taggtaaaga atctaaattt tgcaataatt cacataaaga ataattataa agctcaggat
    12301 atttttcaag agcagcattt aatttatcaa cataagcttg ataatgctta tcatgatgca
    12361 gtttcattgt ttctttatct atataaggtt caagtgcatc atatgcataa ggtaatggtt
    12421 taaccttaaa tttgttattt tcaggtgtaa aagcaaatga agttatgcac tgtgagatta
    12481 taaataaaga cataataact ggtattaata tttttttctt cataaaaatc acctcctaat
    12541 atagtatttc tagaaataaa aaaaatatga aatattaaca gataatttat aagaatttaa
    12601 tagcaatttc attttaaatt gaataagttt taaagccaaa gacaaaacta tgaaatatag
    12661 aataagacta aaggacagaa aggaattttg attatatgga cggcaatatt tcaatttatt
    12721 ataaaggtga tatatctaaa aaagaagata ttaaatttaa aataaaagaa tctgtaaata
    12781 atacaagact ttctagtaga atagagtata aggatgagga aaaaactttt gattttgaaa
    12841 gatttaatgt aattgatgct atttatggaa ataaaccatt tgaaaaagat ggaaaagtta
    12901 ttgtttttaa tggaaatata tataactatc atgaaataaa agaagaatta attaaaaaag
    12961 gacacaattt tactacaaat gatgatattg aagtcttatt ggcatcttat atagaatttg
    13021 gtaagaattg tgtacataag attaaaggaa tgttcaactt tataatctat gatagagaaa
    13081 atgaaagtat atttggagca agagatttat ttgggataaa accactctat tatataaata
    13141 aggaaaatgc aataattttt tcctcagagt ataagttttt acttgaatat atgaaaaatt
    13201 taaatataaa tgaaagaagc ttacaaagtt atttttcatt tcaatatgta cttcctgaag
    13261 atactatgat acaaggaata aggctgatac cagcaggtca ctattttagg gtagaaaatg
    13321 gtatactatc tttaaagaga tataataaac ttgagtttag aagtagtaca aaattttttt
    13381 atactaaaaa tcacttaggc aatagagatg tgaacgagga tgatataaga aatatagtag
    13441 tagattcaat agtaactcat atggaagagg agaaagaaat tggaacattt ctgtctggtg
    13501 gaatagattc atctattata actactgtgg cttcacaaat taatccaaat ataaaatctt
    13561 tttctgcagg ctttagtgtc aaaggatata gtgaattaga agtagcaaag aaaacagctg
    13621 ataagttagg tatagaaaat atccagatta atataacaca agatgaatat ataaaatcat
    13681 taccgaatgt aatttattct ttggatgacc ctatagcaga cccttcagaa gtaggtcttt
    13741 atttcttaat taaagaggca ggtaaacatg ttaaagtagc attgtctgga gaaggagcag
    13801 atgaattatt tggaggatat aatatatata aagagtatag tactatgaaa tcagttgtca
    13861 attcacctac ttatattaaa agtatattag gaagagtttc agaacttatg ccaaacataa
    13921 aaggaagaaa ttatctttat agagctacaa caccattaga aaaaagatat ataggaaatg
    13981 caaaagtttt tgaaaacagc gaagtaaaaa gattcttttt taagtataag gaaaagaata
    14041 tatatgaata cctgttgtct aatttgtata gagatgcaca aaaaaataat tacgattata
    14101 taagtaaaat gcaacatata gatgtgaata catggcttca aggcgatata cttcaaaaaa
    14161 ttagcaaact ttctacagct gagcaggttg aacttagagt accattttta tataaagatg
    14221 tatttgatgt agcaaaaaat ttaagaatgg agcaaaaaat aaataagaat aatacaaaag
    14281 ttttacttag agaggcgttt agagaaatag tcccagaaca tgttgttcaa aggaaaaaat
    14341 taggatttcc aactccaata agagtttggt taaaagattc tctaggggat attgtaaagg
    14401 aaactattta taattcaaat gtagatgaat ttatagataa aaaatatgca ataaaacttt
    14461 tagatacgca tcttaaaggt catagagata attcgagaaa aatatggact atatttacat
    14521 tttgtctatg gcatcaatta tttattgagc ataaaaatgt cgagtattaa tacttttttc
    14581 aatattaatc agagggcaaa agcatgcaat gttttcaccc tcctgattat tatggaatag
    14641 gataagtaga attttgattg attagtttac tatctcaata aaattattgg caaatatttt
    14701 gacaagatta ctttcaacag ctaaatttaa gttttgtcca tcatcatatc cagcggaaat
    14761 caatcctagt aagtttccaa acatatctaa aagagctcct cctgaactac cactagagat
    14821 tggagctgta aattgtatca tttgaaccgt ttcaaaatca cgaaaagctg aaataattcc
    14881 atctgaaact gtattaaaaa gacctagagg gcttccaatt gcaactattt tttgacctct
    14941 cacaggtttt ttactgactt taacaggtat tggcttgcat tttctatcaa cctttatcac
    15001 agctaaatca taatctgaat ggtattttat aatttggtaa gatgtataaa ctttatcatc
    15061 attttcaaat ctaactaaaa atgaataccc atcattgact acatgaagat tcgtaagtat
    15121 ataaccttca ttatttatta ctactccaga accgctctta aacggtttat tttcgttatt
    15181 gcacacaaca atcataacaa cagaagctga taactttgca aggtcttcat aagaaagttc
    15241 tacttcctca tcttgtacaa tttttttaga tgttagattt gtgttattaa gtgtattttt
    15301 agagttaggt ttattcgtag taggagaagt cttattggta ttttcactgt aatttatatt
    15361 tgtaatatgc ctttcaaaag gtatttctaa tatatttgga gtcttcctag aaagttcaaa
    15421 tagagtagaa aattttgaac attcaggata taaatcaaga atatctttat ttggatttat
    15481 atataaaata tcacacccaa gttgtgataa aaaatataag aaaaaaagtt cttgcctttt
    15541 aatatttcca aagtatacta tctttggtga aatagtagtc ttcaccttgt tataaaataa
    15601 tttaggtaga aaatagtcta tccaacacaa aagtttaact gcaaagtttt tgatgataga
    15661 ggatgtacaa tttggcatac ttgtggaaaa taaatctaaa gtatttttaa atgcaatctt
    15721 tttagtccaa tttaaggtgt cattttctat tttacatgga aagtcgatat tatataaatc
    15781 ccaactgtta aacttagaac tattataaat attaaaaatt ttaagcatct tatctgtttg
    15841 ttctttgtca aaatttattt ttagattgtt taatctaata tagggaattg taggattatt
    15901 aaaaaaataa gtatctatat ttttaatttt ttctatgtat ttatcaattg aatcatcaac
    15961 acctatatat cttgtaaagt agacttcttt aatatatgaa gaacgcttat ttttataggt
    16021 agtatcagat ataaattcaa ttagaggttc agtagacttt tttattgaaa tctttaatgg
    16081 tttaatagta caaatcatat tttttctcct ataataagat gatataatct attataaaac
    16141 aacttaaaac aatatagtac aataattttg tagtttcaaa ataaaatatg tacttgttta
    16201 tataaatgat atataattac atattaaaca tagcttttat gcatttaata aagtatgtaa
    16261 aatctatgta aaaagtaaga aatataaaca tttttttaat tatattttaa aaatatcaag
    16321 caagttttat tcacaaatga ataaaaaatg ttataatatg attaaagaaa aagataaata
    16381 attagcaaga tttgtttagc taaaattaac aagggagcga taaattatgg gtataacatt
    16441 atcaaaaggc caaaaggttg atttaactaa aggaaatcca ggtcttaaaa atattttagt
    16501 aggtttagga tgggatacta acaagtatga tggtggattt gactttgatt tagatacagc
    16561 agcattttta acaggagcaa gtggaagtgt tactaatgat ggagattttg tattttacaa
    16621 caatttaaaa catacatctg gagcagttga gcatctagga gataacagaa ctggcgaagg
    16681 tgatggagat gatgaacaaa tagttgttga tttatctaag attcctggag aaataagcaa
    16741 gatttctttt actgttacaa ttcatgatgc aacagaaaga agacaaaatt ttggacaagt
    16801 aagtaattct tatataagaa tagtagataa agacacaaat gaagagttaa taaagtatga
    16861 attaggagaa gattttagta tagaaacagc tatagtagta gctgaaatat ataagcacaa
    16921 tggagaatgg aagtttaatg cactaggttc tggctttgaa ggtggtcttg ctgcattatg
    16981 tggaaacttt ggaataaact tataatttca actataacaa taaactaaag ggggatattt
    17041 tccatggcta tagtattaaa aaaaggacaa aaaatcgatt taactaaggg aaatccaggt
    17101 cttaaaaata taaaacttgg actaggatgg gatacaaatt catttgatag tggctatgat
    17161 tatgatttag atgtaagtgt tttcatggtt ggagaatctc aaagagttga aaaagatgaa
    17221 gattttatat tctacaataa cttaaaacat ccatctggag cagttgaaca cttaggtgat
    17281 aatagaactg gtgaaggtga tggagatgat gaggaaatat tagtgaattt agagacaatg
    17341 ccaaagcata tacaaaagat agctgtaaca gttacaatat atgaggctgc tgaaagaaga
    17401 caaaattttg gacaagtaag caactcatat ataagagtat taaattctga aaatgatgaa
    17461 gaaatattaa gatatgactt aggtgaagaa tttagtatag aaactgcaat tacagtatgt
    17521 gagatatata agtataatgg agaatggaag tttagtgcag ttggagcagg atttgaaggt
    17581 ggcttagaag cactttgtag aaactatggc ttaaatgttt aatttaagca atataatata
    17641 ttaaatataa ataacctata caaataatgt ataggctata ttatataaaa ttttgtggag
    17701 gtttagtatg tcgattaatt tatcaaaagg tgataaaata gatttaaaaa aatctaaccc
    17761 aggactgagt aacatacttg taggtctagg atgggaccct gtgcaacaat ctggcggagg
    17821 tttttttaaa tcgttatttg gtggaggaca agctgatata gattgtgatg catctgtatt
    17881 tatgcttaat caagaaggta aattaagtgg aataaaagat ttaatttatt ttggaaatct
    17941 aaaaagtgcc tgtaaatcag ttttgcatac aggagacaat ctaactggtg agggtacagg
    18001 tgatgatgaa caaatcctag ttaatttgga taaagttcca tcaaatattc ataaattatt
    18061 gtttgtagta aatatataca attgtgtaga tagaaaacaa cactttggaa tgatagaaaa
    18121 tgcctatata agagttgaag accaaggtaa taagaaagag atagcaaaat acaatctatc
    18181 tgataattat tctgaaaaaa ctactcttat agtaggagct atatatagaa aagatggaag
    18241 ttggcaattt aaagcaattg gtgaaggaac aaaagatgcg ggtcttaaag aagtaatgca
    18301 aaatttagac aggattgagt gtgcatatgg aatttaataa tgatatcaaa tattataatg
    18361 tagataaaga agaagttaca aaagacttat ctgttaatcc tgaaaaaggt ttatcagaat
    18421 cagaagtcaa gactagaaga gaaaaatatg gtttgaatga atttactcca aaagaagagg
    18481 gtagtttttg ggatgattta aaggaaagtt taagtgaacc aatgatagtt atcttgatta
    18541 ttgcagcagt agtaagtgca gtaattgggg aaactcatga tgcaataggg attgtaggtg
    18601 ctatagcaat aggtatagca ataggtatga ttacagaagg tcgttctaag aaagcagcag
    18661 aggctctttc taagttgact gaaaacatag aagtaaaagt attgagagat ggggaagtac
    18721 atcaaatttc taagagtgaa ttggtaccag gtgatatagt atatattgaa actggtgaca
    18781 tggtacctgc tgatggtaga cttatagaaa gtataaacct taaaattaga gaagatatgt
    18841 taacaggaga atctgatgac gtatctaaaa agtgtgatgt agtagtttct atggaagaga
    18901 tagaatctaa aggagcagta gtagtacagg aaccaatacc tgcaaaacag ataaatatgg
    18961 tgtttggtgg tacacttgta gcttatggta gaggagctct agttgtaact tcaattggag
    19021 ataatagtga aatgggtaaa attgctcaaa acttatcaga aacagatgag caaactccac
    19081 ttcagataaa acttggaaat ttaggtggtt tgatagccaa agtttctagt gcagtagcag
    19141 gattactatt tatatttatg gttttccaaa tgataagtaa gaatgtatta aatgtagata
    19201 tgtctggaat attaccgttt ttagaatcaa tagacccagt taaaacagca tttacagtat
    19261 gtgttgcgtt gatagttgca gcagtgccag aaggtcttcc aactatgata aatatgacac
    19321 ttgcaataac aatgcaaaaa atggctaaaa taaatgcatt ggttaccaaa aaagaagctt
    19381 gtgaaacgat aggttctgtt tctgtaatat gttcagataa aactggtaca ttaactgaaa
    19441 atagaatgac tgtagaagtt gcatatgttg atggcagata tattgaaagt tcagaagagg
    19501 aaataaatag ttattttgaa gaaaactgta tgataaactc tacagctgat gtagagcaca
    19561 atgatggtga tattaagtat ttaggaagtg ctactgaatg tgcattgctt ttatattaca
    19621 aaaacgtaga ttatagacaa gttagaaaaa attctaatat agtagctcaa aatcctttta
    19681 cttcagattc aaagagaatg acttctgtta taggtcaaga taatcatcat gtactattat
    19741 caaaaggagc accagaagtc ttacttgaat tatgttcaca tattcaaaga ggaaaagaca
    19801 tagttccatt aactgaaaat ataaaacatg agattttaga agaaataaag aaattgcaaa
    19861 taaaatcaat gagaacttta gggtttgcat ataaagaaat ctcacaagct gaagaagaag
    19921 cagcagtaac tgctgaaagt gatgctatga atgtaagtgc aatggaaaac aatttagtat
    19981 ttagtggttt tgttggtata agagacccac ttagaaaaga tgttatagag tctgtaaata
    20041 cagcaaataa agctggtgtt tctgtaaaaa tgcttacagg agataatata aatacggcaa
    20101 gagctatagg tgaagagtta ggcctattaa aaaataatat gagagtggtt gaagcttcat
    20161 atatagatac tttagatgat gaagaattaa aacaagagat acaaagtatt tcaatcgtgg
    20221 caagaagtaa accagatagt aaaatgagaa tagtttcagc tctgcaaaag agcggagatg
    20281 ttgttgcagt aacaggtgat ggtataaatg atgctccagc actttcaaaa gcagatgttg
    20341 gtatagcaat gggtatatct ggtacagagg tatctaaaaa cgcagcagac ataattttaa
    20401 ctgatgatag ttttagtacc attgtaaaag gtattaaatg gggtagagga atttatgata
    20461 acttccaaag attcgttcaa ttccaattaa ctgtaaatgt aattgcattt ttagtggcta
    20521 taatatctca agttatggga caagagatgc catttactac aatacaatta ttatgggtaa
    20581 acatcattat ggatggacca ccagcactag cattaggttt agagccagtt agggaccatg
    20641 tgttaaacag aaaacctgtt gatagaaaag ccaatataat ttctaagtca atggtgtata
    20701 caatagtatt aaatgcattt tatataacag caatactaat gttacaatct acatttaatt
    20761 tcttaggagt tgatgaagcg cataagggaa cagtaatgtt tgcgttattt gcttttagtg
    20821 cactgtttaa tgcatttaac tgtagagagt ttaatgcaga tagtatcttc cctaatttta
    20881 cacataacaa attagcacta caaataatag tgttgacagg aattgcacaa gtaatattta
    20941 ctcagctatt ccaagatttc tttaactctg tagcaatgga ttttatgaca tgggtaaaag
    21001 ttttatgtgt agcagcaagt gtaattgtgg ttaatgaagt tgtaaaatgt ataataagac
    21061 tggtaaaacc aaaaaagact gcttaaaaca aattttttag aagaaaacag actgactcaa
    21121 aatatgaatt ttgggtcagt tctgaataaa catttaaatt gggaggatag ttatgggtga
    21181 ctcaaaggta tttgaaaaaa tattctccag ccaatcagat agaggaaatt acactccttc
    21241 aaagggatat ctaagttatt ttatttcgta tattggttta gaagatgaag ttttgtacaa
    21301 tctcgagata tttaaaacaa aacaaaatat tgatagcaaa aaagatattg ctttatttac
    21361 tgatgttata gcaaatccta gtgattttga tattataaat tattttaaaa gtggacttca
    21421 aaaatataga actagcatgg aagatgtaga tataaatatt ttgggatttg aagagattga
    21481 ttataaaata aagcaagcaa tggatagagt gttaaaagaa gaggaaaaag aatttactaa
    21541 tgatagagtt aaacaaaatt ttatagtgaa aataatggca tggataaaaa tttatatcgg
    21601 tgctttagat ataaataaaa atgaagctcc aaaagtaata ttttatggag atataaaaaa
    21661 acatgaagtt tatttactct taatattata cttggcaggt tttgatgttt tatatcttaa
    21721 tccaaattca aagagtaata tagatatttt aaaatcagaa agatacaata tagagtttga
    21781 agaagccaac ataatagaag aaaaaatttc ttttgaagaa agggttattt taggggaaaa
    21841 aatagataaa agttctgtaa aaaaagcttt tactgttggg gcagaagcat caaagcgtat
    21901 aagtgaggaa ttgttaaatg atgcaggatt tataaaacct tggcaactcc aagatagaaa
    21961 aataaaaaat ctacttctaa gttcaactgt tgatgaaata tcaatatact ggaatcaacc
    22021 acttaaatta agaccaggat ttaagtttaa tgatgctatt gtagaagctc caaattttct
    22081 ttctaagatt aatggaatat ataacgataa gaatgaatat attaagtttt tagatttatt
    22141 aagagattcg gaaagttcaa catttattga atttaatgga gatgtagata gattttcaaa
    22201 agcttttaca agagaagctt ttagtttatc ctttttattg gattcaaaag gtgcaataga
    22261 taaaaactct gttttaaata ataaagatta ttccatatca acattagcat tgaatcaaca
    22321 aattatgatt ttagagaaag tggaagagtt gttagaagga agtatgtttt tgaatggttt
    22381 aagtggagaa gacaagataa aaggtctatt tacagtactt cacatggata agaaatttgt
    22441 tcatatgatg aacaatttcg attactcact tataaaccca aaattgatta tatacatgta
    22501 caagagtatt gtatttgata aagaaatagt ttttcttatg cttttactta gtaagattgg
    22561 atttgatatt atcatattat gtccaggagg agaaaacaat atagagaatg ttataaataa
    22621 ccaattgatt gatgtacaca gattggataa aatggtatat gaccttaaat taaattcgct
    22681 tgaaaatgat atacctttac ttaaaaaaat ttttggaaag aggagacgat tttagatggg
    22741 gataaatata aatagttttt caaaagataa gaactctgta gaggttactg agaatttgcc
    22801 tacagcagtt caagaagaaa acttcgatat aatggaatat acaaataata aaaaaaatga
    22861 actgagaaag tctaaggaag ttgaggcatt gacatctctt atagaagtag aaaatccaga
    22921 tacgatttta cagtttggta ggaaagcatc tgaaggagta gcaagagttt ctgatagcct
    22981 tttaaataca ataaaattaa atagaaatga agaaaattct aaaatgctgg ttcatttaac
    23041 taaaataatg gacaagtttg atttagacga ttttcaagaa actaaggaac caaatttcgt
    23101 tcaaaaactt ttcaaaaaag ctaataatgc cattgaaatg atgtttcaaa agtatgaaac
    23161 attgggagga gaagttgaga aaattcaaat tgagcttgaa caatacgaaa gagatatagc
    23221 actttctaat aagcaaatag gtgctatgtt aaatgaaaac tttgagtttt acaatgaact
    23281 tcaaaaatat attgttgctg gagaaatggc aatagaagaa atggataatg aaatactacc
    23341 tttctttaaa caaaaatcag agactagtgg agaccaaatg gatgttgtca attatcaaga
    23401 actattaaaa gtttatgata tgttaaatca aagagtctat gatttaagga tagcagaaaa
    23461 tatagctatc caaacaatac ctatgcttag aggtatgcaa cataataatt atggtttaat
    23521 tagaaaaata aatagtgcat ttgtcgtaac tttaccagta tttaaacaat gtttatctca
    23581 agctatatta cttaaaaagc aagaattaca agcaaaatct ctaaaagcac ttgatgacaa
    23641 aactaatgaa ttattgctta gaaatgcaca aaatgtaagt actcaaagtg cacaaatagc
    23701 tagaatggct ggaacaagtt ctgttcaaat agaaacattg gaaaaaatgt acaatactat
    23761 taaatctggt atagatgaaa ctatgagaat agaagaaaac aatagagctt taattaagga
    23821 taatacaaag agattggaag aattaaatac gactataatt tataataagt agtattatat
    23881 taagggctaa tttgtataaa gttggccctt tctatgtata tgaggaggct agacattgaa
    23941 atatttcgat tatatctgca aagatgattt ggagagaatt tttttgaagg agccagaaga
    24001 ttttagtgct aagactgaaa aagatgtgtt gaaatatgca ctgggcgctt ttttatacgt
    24061 tccagctact caatataata tgatttataa gagtattata ggggatgtta agggagttag
    24121 accattagct atttgtttag aagatgctgt tggagtaaat ggagagttag aggctataga
    24181 aaatcttaga ttaattttaa aaaatattag caatgaaagt ataactaata aagatggaat
    24241 accactaatt tttgtaagaa taaaagatgt ggagcaactg ttgagaatta aagagattat
    24301 aattaaaaat agacattcta taacaggaat attaattcct aaagcaaatt cggagttaat
    24361 agaaaattgt atagaagctt tagattctat gaatcttcaa gatatgtatg ttattccgat
    24421 aatagagaca aaagaattta tatataatga aaaaaaagaa cttagtttta ctaatttgta
    24481 caatgctatt ttaagacata agtcaagaat attaagcatt agagtaggca taactgatat
    24541 attagggatg tatggtataa ggagagataa gaacttctct atatataata atttgatttg
    24601 ttcttcattt atattagata taatcacata tcttcaaaga ccagagttag atatacctat
    24661 aagtggtgga gtatcagaat tttttgatat gacgaataag gatataagaa ataagtatat
    24721 agaagaaata ttattggata aatatcatgg attaattgga aagaccgtaa ttcatccaat
    24781 gcaaattcaa attgtacaag cgctatctac agtatcatat gaagatttta ctgatgcttt
    24841 ggatatactg gatagtacag acagtaagta tggtgtgagc aagggtgtat taggagagag
    24901 aatgaatgaa acaaatccac attttttatg ggctaaaaaa acattgattc tatctaaaat
    24961 atatggtgta ttaaataaag gagtagacta tgaagaattg cttaaatttt aattgtgaga
    25021 ttgacattat tgataatcca ttgaatttag aactaaatga ttttttagac gtaggaataa
    25081 gaaataatag caaaagacgt tttttgttta tctcaaagaa actcggtaaa catttagcat
    25141 gtaagccaag tgagatggat aatttgggaa aattaatggc tacaatttat aatgaaaaaa
    25201 ataaggaaga atcaaatggt actgttatag cttttgctga aactggtaca gctgttgggc
    25261 atagtttttt tgattactta tgtggagatt atgaatttat tcatactact agagagcagt
    25321 ttgacgagtt agaaaatctg gaatttttag aggaacattc ccatgcaaca gaccagaatt
    25381 tatactttgg tatgcttaac aactttaaat ttggagatga gataatattg gttgatgatg
    25441 agattaccac agccaataca tgcatgaata taattaggaa aattcaaagc atttatccta
    25501 aaaagaaata cactatatgc tcaatactca attgggtaag tgatgaaaat ttaagtaaag
    25561 ttactcgttt agaaagagag ctagattgta agatagattt tgtatactta tttaaaggaa
    25621 actttaaatt ttatatagat gaagatatta tttcaaatta caacgataat tatagtatta
    25681 ctaaattaga gactatttca agtgataaag taaatttagt agtaaatcat atttatgttg
    25741 atttagaaga ttatattgga aataagaaat atgtaaaata tacaggaaga tttggaatta
    25801 atagaagaga acaatttaga ttaaaagaaa tcgttaagag agaaagtaaa aaaataacta
    25861 tggccagtaa agaatcttta aaaaatgaaa aagaaaagat tctattttta ggtactgaag
    25921 aatttatgta tatacctatg ctgtttgcaa aagaaaaaga aaatcaatat gatatatact
    25981 atcattcaac aacaagaagt ccaattatta atattgataa gaataattat cctattaaaa
    26041 gtaaatttat gctaaagagt ttttataata tagatattca aaattatatt tacaatatag
    26101 ataaacataa tttttctaag tgttatttat ttgtagagct gaataaaagt gaaaattctt
    26161 atctagaatt tattgatata attaaaaaaa caagtataaa agaattaact atagtatgtt
    26221 gtagctaaag tgttttaaca ggataaaata tatcacgatt ttaagatagg agttgagtca
    26281 tatgagagat tttagcacgt attctaaaga tgatgttatt tttttattaa aagatatatc
    26341 aaatttaatt gaagaagaag gaaataaaga aagagaatta aaaatacaaa atggtagaca
    26401 ttattcagaa atgataccaa tagagtatga agttagtgaa gattatctga atttatatca
    26461 tgagaaacta agagaaaata aagataaact tgcattttct ataggagtta tgtgtgaaaa
    26521 aataattaaa aaaaatggaa atgatgtagt actagtttca cttgctagag caggaacacc
    26581 tattggtatt ttggcaaaaa gatatataag aagcaagtat aatgtaagtt tacctcacta
    26641 tacaatctct ataataagag ataagggaat agatatgaat gcaattgaat atatagtaaa
    26701 gaatcatccg agctcaaaaa ttcaattttt agatggatgg actggtaaag gaactatatc
    26761 aaaagaactt gaaaaagcat gtgatgaact taatgttaaa tttaaaaaat attttgactc
    26821 aacacttgct gtattagctg accctgctgg atattcagga ctttatggaa ttagagaaga
    26881 tttcttaata ccaagtgcat gtttaaattc aacagtttct ggtttagtaa gtagaactgt
    26941 actaagagag gatttaatta gtaaaaatga ctttcatgga gctaagtttt atagacattt
    27001 aaaagataaa gatgaatcta tgaactatat cgaaactata gaagaatgtt ttaaaaatca
    27061 atttaagaat atatctgatg aagttgaaaa ctgggaaagt gacattataa caagagatgg
    27121 atactttgat gttttaaata taaaagaaaa gtataacata acagatatta actttatcaa
    27181 accaggtgta ggggaaacga ctagggtttt acttagaaga gttccgtata agattcttgt
    27241 taaagactta aatgataaat ctttagacca tatattcata ttagctaagg agaaaaatgt
    27301 tgaagttgaa caaatggatt taaaagccta taaatgttgt ggaataataa aaaatatgaa
    27361 agatatttag gaaatatatt agacaggaga atattgaatg attgtttttt cagatttaga
    27421 caggagtatc atttactcca ataaattttt aaatgctgat agtaaatatg ctaatataga
    27481 aatttatagg gaaaaagaaa tatcttatat atctttagat actataaatt taataaagca
    27541 aatacaatat tatggtatgt ttatacctac aactactaga acagtggagc aatttaagcg
    27601 tatagaattt aataaatatg ggatttattt tccttggagt ataacttcaa atggaggagt
    27661 tatacttaaa gataatgaaa tattgaaatc atggagtgaa aaaatagata aactaaaatc
    27721 tgattatgag cccattgaaa gtatgattca taaatttaaa gactatttga atgtagatgg
    27781 aataactaat tttaaagttg cagaagatac atttttttat atagttgttg atttatcaag
    27841 atttaatttg gattcaatca aagaatatac aaatatattg gaatcaaaaa attggaagtt
    27901 ttatgtgagt ggcagaaaaa tatattttat accaaaagaa atttcaaaag aaaatgctat
    27961 aaaatacttg actaaagaat taggaataga atatttttat gcagttggag attctattat
    28021 ggactatggt atgcttaata tctcaaataa atcctatgta ttgaagcatg gtgatataaa
    28081 taaaaatgaa atagaaaatt catttatatc aagttctttc aatggaatga gtggaactga
    28141 agagatacta tctaacattt tagacgagaa ctgcctactt aatatataat gactgatata
    28201 taaataataa actagaaaaa tctgaaataa attatagtaa agtttatcat actaatttat
    28261 ttcagatttt tatcaattta tatttaagta agaactcaat atgttaaaat gaaggtggag
    28321 tcattgccca gactgagata catacctcat catcataatt ttcatacaca tgtggtatag
    28381 tactgttaaa ataaaaacta tctccttcct ccaatagata ttccttattt ccaagtgtta
    28441 tcttcatttt accttttatt atatagccac attcttctcc ttcgtgtgtc acaaattcac
    28501 tgtttttgtg tgtatgacca tctagtgtaa caagtataaa ttctattttt ttcccagata
    28561 aattaggaac aagaagttcg taaaattttg tagaatcatt agttactatt ttttttcgat
    28621 tatttttctt tactactata tcagagtctt ctcttccaat ctcttcaaaa aaataaccta
    28681 tatttatatc aagagctttt gatattttcc acattgctac aatagatgga acaaccatat
    28741 tacgctcaat ttgacttatt aagcctgtac ttaaatctga attttttgct aaagtagaga
    28801 tacttatatt tttagatttt ctaagagatc taattttgct tccaacatct atattcatta
    28861 gatacaaacc ccctcagaca tgattataac atataattta taaagattaa taaaataaaa
    28921 aaaaagtttt acaatacaag tattttttat gctatattga attttctgaa aagatgcatt
    28981 ataatagtat taatattcaa tatattgaat attgataacg atattttaat ttaatgaaat
    29041 ggaggtaatt aagatgaaga gttttgtaag tcctgataat atttgggtat tatgggcagt
    29101 tgtaactgga tgggcagcat ttagtattta tttggaacaa aagtacaatt gggcatcaaa
    29161 agtgtcggga gcaataatag ctcttgtagg agcaatgtta ttatcaaatc taaatattat
    29221 accagtagag tctgttgtat atgaccaagt atggggatat gttgttcctt tagccatagc
    29281 tttattgtta tatcaatgta acattaaaaa gatatggaaa gaaagtggaa gattgctcat
    29341 tatatttctt gtaggttctg taggaactgt acttggagct atgattggat ttttagcatt
    29401 aaaaaatgta gtaccagatt taaatattgt tgcagctatg atgacaggtt cttatatagg
    29461 tggaaatgta aactttgctg ctatgtcagg ggcgtttgat gcgccaggag aattagtatc
    29521 agcaacagtt gtagcagata atcttttaat ggcattatat ttctttgtct taatagctat
    29581 accatcaatt ggatttttta gaaaacattt taaacatcca catgtagatg aaatggaaag
    29641 tataggtata actgaaggag aaacagtagc agcaaattat tggggaagaa aagaaatatc
    29701 tttaaaagat atagcaatag ccattggtag cgcttttata attgtcgcag tatctgtaga
    29761 actatcaact tggtttaaag ctataatacc attatcaaat ccttttttag caatgttaaa
    29821 tactttattt ggaaatcaat atctaataat aactactttg actatgttgt ttgccacatt
    29881 tatgcctaat ttctttggtg atataaaagg agcacaggaa ttgggtacat ttttaatata
    29941 tatattcttt gttgtagtag gtgttcctgc ttcaataaca ttaataattc aaaaatcacc
    30001 attactactt ttatactgcg gaataatggt actaataaac atgctagtta catttatagt
    30061 tgcaaaaata tttaagttta gtttagaaga agctattctt gcatctaatg caaatattgg
    30121 tggtcctaca acagcagcag ctatggcaat atcaaaagga tggtctaagc tagtagggcc
    30181 aatacttata gttggaacat ttggatacat agtcggtaat tattttggtc ttttagtagg
    30241 aaatattttg atataatttt acgtgaaaaa aagttgtttt ttagaaagat ttttatttaa
    30301 ttggaggttg ttatgatttt cgatggacat tcagatactt gggcagatgt aacaataaga
    30361 aggcttaacg gggaaaaaga tgtatttaga agaatccatc ttgataattt taaaagagca
    30421 aatatagaag gagctatttt tgtaatatgg atagacccac catatgataa agagcctgaa
    30481 aaaagagtta aacaaattat aagctctata aaagatgaaa ttgaagatat gaaagatgtt
    30541 gtaaacatag taaaaaaata ttcagatata gataaaggta taagtgaaaa caaaataaat
    30601 attttaattg gtcttgaagg attaagccat attggaagaa atatagattt actagattat
    30661 ttttataaag aagttggtgc aagacatgca tcattaactt ggaatgaaca gaatgaactt
    30721 gctacaggat gtttagggga tgaaaatcgt ggtttaactg aaataggtaa aaaagcagtc
    30781 aataaaatgg aaaaattggg catgatagta gatgtgtcac atttaaatga aaaatcattt
    30841 tgggatttaa tgtctgttgc aagtaagcca gttatagctt ctcattctaa tgctaggagt
    30901 ttatgtgatg ctaaaagaaa tcttacagat aatcaattaa aagaaatttc aaatacaggt
    30961 ggattagttg gaataaatag ttttagagga tttactaata gtaatcctaa gaagcaagat
    31021 ttaatacatt tggttgacca tatagactat atggtaaatc ttataggtat agaacatgta
    31081 tcttttggtt ttgattttag tgattactta gggaaagaga ctataggttc ttttactgga
    31141 aatgctagtg tgccaagtgt agatggtctt gaaagcacac atacagcaca gaacatagta
    31201 gtagagcttg aaaatagagg ttataaaaag aaagatatag agttaatctc tagtggtaat
    31261 ttttataggc tctttaaaga tatactaaaa taatataata aagaacttaa atttataaaa
    31321 tagtatattt atacaaataa tatctacata tttaaattgg cgttcataat ttattaaatt
    31381 aaattttaat gttactagaa gtttcaattc tagtaacatt ttttatgtta aaaacagata
    31441 tagatatata ttgacaatga taattattat tgataaacta taaaagtgaa aatgataata
    31501 attttcattt taattaataa aagattttta aggagggaga atgaagaaaa ggtatttagt
    31561 cataggaata ataattttgt cttatttttc tttatttata ggagctgaag atataaatat
    31621 aatgcatata tttgccaaaa atcagcataa acttatgata tttatcatga gtagaatacc
    31681 aagattaata agtatattga ttgctggtgt aggaatgagc atagcaggtc ttataatgca
    31741 acagataagt aagaataaat ttgtatcacc tacaacaggt gctacaatag atgcagcaca
    31801 atttgggata gtaatatgta tgcttttagt accaactgcc tctattttta caaagacaat
    31861 aatagcattt gtattttctc ttgtagggac ttttacgttt atgaaaataa tagggaagtt
    31921 acaatttaaa aatataatct ttgtaccatt agtaggtata atgtttggaa atataatagg
    31981 ttctatgacg gattttatcg cgtataaata cgatttgagt caaaatgtta gttcttggat
    32041 gcaaggtgac ttttctatga tacttaaagg aaattatgag atattatata tcacaatacc
    32101 ccttataata ttagcatata tttatgcaaa taaatttact gttgttggta tgggtatgga
    32161 ttttgcgaca aacttaggtc ttagttacaa aagaattgtc aatataggtc tgataatagt
    32221 agcacttgtt acagtatgtg tagtcgttac agctggtaat atccctttta taggtctaat
    32281 agttcccaat atcgtatcct tgtatatggg tgataatata agagcaagca tatggtatac
    32341 tggtttactt ggagcgatat ttgtacttat atgtgatatt tttggaagaa ttattattta
    32401 tccatatgaa atatctatag ggcttacagt tggagttata ggaagtatac tattccttta
    32461 tttaatatta aggaggaatg taaatgaagc ttagtctcaa tcaaaaaatt tactttgtaa
    32521 gtatattact attcatatca atagcacttt ttataattta tggaatagac atgaatcatt
    32581 tagaatatgc attatcacaa agaataccaa aaatatttgc tatggtatta ggtggaggat
    32641 gtatagcatt tactactgta gtgtttcaaa caattacaaa caatcaaata ttaacaccaa
    32701 gtgtactagg tttagattca ttgtatgtaa tgattcaaac tataatagta ttcatatttg
    32761 gttcaagcag tagtctgatt ataaatgaaa attataattt tattattaat gtagctatga
    32821 tgattggagc ttcattgtta ttatataaga cgttatttga aaaaaataaa aataatattt
    32881 tctttttaat cctagtagga atgatatttg gaaccttatt taaaagtgca acaacattta
    32941 ttcaggtaat gatagaccct aatgaatttt tagctttaca aactagtata atggcaagtt
    33001 taaacaatat aaacactaat gtattgttaa tagcatttat aataattatt gccataatac
    33061 cttttatata tgatgaaata aagtatttag atgtattgtc gttaggtaaa gaacaagcaa
    33121 ttaatttagg tgtagatttt gataaagcag ttaaaaaaat gataatatta atagcaattt
    33181 tagtctcaat atcaacagca cttattggac ctatgacatt tttaggactt ttacttgcta
    33241 atataacaag agaaattttt aaaacatata agcatacata cttgatttcg ggttctatgt
    33301 tgatagggat gattacattg ataataggtc aattttttat acaacatgta tttaagtttg
    33361 atacaacttt aagtgtagta ataaatttta taggaggaat ttactttatt caattactat
    33421 taaagggggc aaatagatga tagagataaa aaatatattt aaaagatata agaataaaaa
    33481 tgttgtagat gatgtctctt ttagtataga aaagggaaag ataacatctt ttataggacc
    33541 aaatggagct ggaaaaagta cagtactatc aatagttaca agacttatag gaggtgatgg
    33601 tggagaagta ataatagaag gaaaaagctt gactaattat agcaataaag aattagcaaa
    33661 aaaaatagct attcttaaac aatctaacaa tataacttta aagcttacaa taagagaatt
    33721 agttggattt ggaagatttc catatagtga aggtaatcta actaaagagg atgaaaatta
    33781 tattgatgaa gcaatagaat atatgaagtt gactgatata caacataagt atttagatga
    33841 attgagtgga ggtcaaagac aaagagctta tatagcaatg gtaatagctc aagatacaga
    33901 gtacatatta cttgatgaac cactgaacaa tttggatatg aaccattccg tacagatgat
    33961 gaaggtactt agaagtcttt gtgatgagtt agataagact atagtattag taatgcatga
    34021 tataaatttt gcatcatgtt attcagataa tatagttgca ttaaaaaatg gaaaagttaa
    34081 aaaagtaggt cgtacagatg agatagtaaa tgaaaaagtt ttagaagata tttatgagat
    34141 gaattttaac attaagaata taaatggaaa tcgaatctgt atttattttt aaaatattgt
    34201 atatgaaaat aaacttgcta tatgattttt tataaagttt ttaaattaaa ataaagatta
    34261 ataggagaga ggaaaatatt gatatgaata aaaaagcagc aatagtagca gcagtagcta
    34321 ttataggatt agtgacagtg tttgcattag gtggaagtaa aaagaatgaa tcaaaaacat
    34381 cagaaaattc aaataatact atgaagataa ctcataactt gggagaaaca gatgtaaaat
    34441 taaatcctaa aaaagtagtg gtatttgatt attcggcttt agatacaatg gatgcattag
    34501 gtgtagctga gaatttagta ggacttccaa aagcgagttt accagcatcc ttagaaaaat
    34561 ataaggatga caagtatgca gatttaggag gattaaaaga accagattta gagggtataa
    34621 agtcggcaaa tccagattta attataataa atggaagaca agaagatttt tatgagcaat
    34681 tgtcaaaaat agcaccaaca ataagtacaa gtaaagatga taagaagtac ttagaatctg
    34741 ttaaaaataa tatagacaaa atagcaaaaa tatttggagt agaagaaaaa gcaaatcaag
    34801 aatttagtaa aattgaaaag aaaatagaaa ctttgaataa aaaagtaaca gataaaaatt
    34861 taaatgcttt aactataatg gtaaatgaag gtaatttaag tgtatttggt gaagaatcaa
    34921 gatttagtat attatacaat agttttggat ttgaaaataa agataaaaat ataaaagaat
    34981 caagtcatgg acaaaatatt acttttgaat atatagctaa acaaaatcca gaagttatgt
    35041 tcgttataga tagaggaata gctactggca gtgatgtaaa agaaagctca actgcaaaat
    35101 ctgttttgaa caatgatata ataaaatcta tggatgcata taagaatgat aatataatat
    35161 atttagattc accaacatgg tatgtaaatg atggtggtct aacatcttta aataaaatga
    35221 tagatgatgc ttcaaaagcg gtaaattaat aatccgatat aatcaaaata tattttttat
    35281 aaaatgatta tcaaataaac taggagtttt taagaactcc tagtttattt tttgcataaa
    35341 aaagatatag tttaaacaat aatattatct ataataagat aaatctattc aaaat
//
LOCUS       DAMJVI010000284        17193 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.085, whole genome shotgun sequence.
ACCESSION   DAMJVI010000284 DAMJVI010000000
VERSION     DAMJVI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17193
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.085"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(249..773)
                     /locus_tag="PC545_003876"
     CDS             complement(249..773)
                     /locus_tag="PC545_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDF2458539.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVRTDSADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(838..1590)
                     /locus_tag="PC545_003877"
     CDS             complement(838..1590)
                     /locus_tag="PC545_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDF2458540.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPEDIKKVYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(1621..2919)
                     /locus_tag="PC545_003878"
     CDS             complement(1621..2919)
                     /locus_tag="PC545_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HDF2458541.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHAVKSTKEILDKNGFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGMLNDTLEKDNFLIKQIASNLDVNMDDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISMGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(2936..5200)
                     /locus_tag="PC545_003879"
     CDS             complement(2936..5200)
                     /locus_tag="PC545_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HDF2458542.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(5455..6273)
                     /locus_tag="PC545_003880"
     CDS             complement(5455..6273)
                     /locus_tag="PC545_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDF2458543.1"
                     /translation="MFKINNLTYQYEKNTNALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEILFKEEKLKYDKRSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGMDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHRNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(6266..6964)
                     /gene="cbiQ"
                     /locus_tag="PC545_003881"
     CDS             complement(6266..6964)
                     /gene="cbiQ"
                     /locus_tag="PC545_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HDF2458544.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLYSFEFFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(6967..7245)
                     /locus_tag="PC545_003882"
     CDS             complement(6967..7245)
                     /locus_tag="PC545_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HDF2458545.1"
                     /translation="MSAKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEITK
                     INPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(7235..7987)
                     /locus_tag="PC545_003883"
     CDS             complement(7235..7987)
                     /locus_tag="PC545_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HDF2458546.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLFGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAIPVFLAAAIGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(8169..8361)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8493..10265)
                     /locus_tag="PC545_003884"
     CDS             complement(8493..10265)
                     /locus_tag="PC545_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HDF2458547.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKVIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYTFVLYSMDKHPALDSMTRRIVNDIRNIDTN
                     QDIMEILKALDVIYLTYFEYILNGEENSLIDENNVESVEVNFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKCSNSADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRNVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKSRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSIKNSENIFEYFCAGNNRDGLAIKSICSGLINRDEENKILIVLSDGRPNDVKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(10279..11208)
                     /locus_tag="PC545_003885"
     CDS             complement(10279..11208)
                     /locus_tag="PC545_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDF2458548.1"
                     /translation="MDIIDNLRVQGVDEKLIEDVLYFRNYYGLEKDLEYRVTKSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIHFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            11437..11625
                     /locus_tag="PC545_003886"
     CDS             11437..11625
                     /locus_tag="PC545_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458549.1"
                     /translation="MEYMSCPEVAKKWGISERRVQKLCEESRIPGVSKLGYMWLIPKN
                     AEKPVDGRTKRRKEVQYE"
     gene            11618..12319
                     /locus_tag="PC545_003887"
     CDS             11618..12319
                     /locus_tag="PC545_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458550.1"
                     /translation="MNSVLIIDDDKELCALMKKCVEQENLSAITAHGGAEGLHLVDKN
                     RHTLSLVILDIMMPDIDGFQVLKKIRETSNIPVIMLTAKNDEDDKVSGLRLGADDYLT
                     KPFSINELMARVNSLIRRYTMLNPAFITDTDFIIINGMIIDKTNRIVSVNNIPVQLTS
                     KEFDLLAFLASNKGRVFTKKQIYTQVWEEEYAFDDSNIMSFISKLRKKIEPDPNHPFY
                     ILTVRGVGYRFNKEA"
     gene            12321..13244
                     /locus_tag="PC545_003888"
     CDS             12321..13244
                     /locus_tag="PC545_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2458551.1"
                     /translation="MNTSVYLLLILALSLIIIAYLVSVLLRVRIQLTLIKDALEDIKN
                     GNLNRRILTDENDITKQICYDINEIAINSQFQLIQQKQSEQAYKQLMTSLSHDVKTPL
                     ASLVGYLEAVESKIVVGEEKDEYIHVASNKAHYLKNFVENLFEWVKLDSKEQVFHFDI
                     FDLNELSRNIISDWIPVLESSHFEYEFDIPEIEYFLRIDANAYTRIINNLLQNIITHS
                     SGNKMTLRIFENKEQAQIIITDNGKGISSDNLPHIFERLYQCDHSRASKGNGLGLAIA
                     KELINAHKGNITANSTPGMGTEFTILLPKAL"
     gene            13401..14324
                     /locus_tag="PC545_003889"
     CDS             13401..14324
                     /locus_tag="PC545_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019639192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458552.1"
                     /translation="MNDFVIETKQLTKIYGNQTVVDKVNLHVKKGRIYGLLGRNGAGK
                     TTIMKMILGLTSITYGEVDVFGENIKGREKQVYPRIGAIIETPGFYPNLTGTENLEIF
                     AKLRGTAAPNSVKRALEIVGLPYKNKKLFSKYSLGMKQRLGIANAILHDPELLVLDEP
                     TNGLDPIGIAEMRKFIRDLSVEHGKTILISSHILSEISLLADDIGIIDHGVLLEESSM
                     SELEKKNSKYILLQVSDIPKTSLILERQFNTKDYSVQDDYTLHLYNTNLDMGEINKAL
                     VMQNVTVISSQLCNDTLEDYFKKITGGEGIA"
     gene            14317..15054
                     /locus_tag="PC545_003890"
     CDS             14317..15054
                     /locus_tag="PC545_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458553.1"
                     /translation="MLKLIQTEFLKLRRRKLIWLMLLSALIMPFFALLYFNYFGKTGV
                     EPIKFYKWSAFGYTLWIILPVVLGIFCTMLIHEENQYDMLKQLWIVPISKMGYFFSKF
                     FVVLIYSICFMLITAVASVLFSVLSGYVVFSWASILYLLKKCLEIGVITAFVMLPILA
                     IATSQKGYILPICITLVYAFSGFVIMTINMYLHPLSSMGVIIMRNKDIPGLIFSQEIN
                     VPLAFLCICIWSIVSVLFANIALGRRK"
     gene            15065..15838
                     /locus_tag="PC545_003891"
     CDS             15065..15838
                     /locus_tag="PC545_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458554.1"
                     /translation="MWKLLWAECQKIRRSKIVWITVFASIMIAVIVFAGGQDVYNDPD
                     LHYGLKSVYDGSRYIDNAGWYMDAVQPWSTFFVLPAVIALLGSYIICREEEEDTIKSL
                     RLIPINETNLTIAKMIITFIFSILLYLLLFTITFLTESVLHFSDLSTKLVLSCMKEYF
                     LDGIGVFFAISPIIALVSRMKKGYWLALVFTEVYSIAGLFAGMSNVLQTFFPIAAIFN
                     ISGYYITTGEKIMGSIMSLLLCACLSAFILKGLKHNEKN"
     gene            complement(16379..16987)
                     /locus_tag="PC545_003892"
     CDS             complement(16379..16987)
                     /locus_tag="PC545_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDF2458555.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVISSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         5559 a         2971 c         2155 g         6508 t
ORIGIN      
        1 tattttatat atttatttaa tacattatta tttaaaaata aatatataaa caatcataat
       61 aaaatttttt taataaaact ttatttttta gttaaaatca tacaatatat agatttccac
      121 aatacaaata aattaaaaag tgaaatgtat ttgtctaaaa acataataac atttcacttt
      181 atcctcattt ttaagtaata tatattttta atttttattt ataactaaat ctagttttta
      241 tataaatttt agtttttttc tacatctgag taatctattc caaaagtttc tacagtaaca
      301 gatttcataa tttgtggagt ttgtggctta tcagctgaat ctgttctaac tgatgctatt
      361 tcatcaactg tatccatacc ttctatcact ctaccaaaac ctgcatactg accatctaag
      421 tgtggtgagt ttttatgcat tatgaaaaat tgagaaccag ctgaattagg tgccatagtt
      481 ctagccattg atatgactcc tctttcatgt tttaaattgt tagtaaatcc attaccacta
      541 aattcacctt ttattgagtg acctggacca cccattcctg taccatttgg gcatcctcct
      601 tgtatcataa atcctggtat tactctatga aatattattc cattgtaata tccttcattt
      661 actaatgata caaagttttt aactgtattt ggagctatat ttggatataa ctcaattttg
      721 atttcttttc cattttccat ttctatagtt actataggat ttttattttc cataatttat
      781 taccttcctt ttcattagtt aaattacata attacattta ttatattata ctctaaatta
      841 aagtctatca agtatatttt ttcctatttc atatccttct tgataaatag ttttacaaaa
      901 ctcatcatca aagttaagcg tgtctttagg agcaaattga gtattaggtc taactactat
      961 tattttatct tcatcatata cttttttaat atcttctggc aactcataat tatattttgt
     1021 ggatattaaa ataattttat ccacattatc cccaattaat ggttcaatta atgcactcct
     1081 aattaaacct ccatctaatt tgaaaccatc ataaattcct tcttgcacat ctttcacaaa
     1141 ttgttcttta aatccaagag ttgcgtttgg atttgatggt aacaacccac tgtatgaaat
     1201 gctgataatg gcttcattac gaggcatttt tgatacatca acaatctttt ctgatactac
     1261 tttattatca acttcaacat aattaacata aaagttcatt tcttctatat tactatcatc
     1321 tagttccctt aggttgtcta tagcaggaga attatccaca gtattattta ctatttttac
     1381 atttccattc tggatatctt ttatatttgt ccacattttc tccaagttat caacattccc
     1441 tgtgtacaaa taatatccat ttattgctcc tattgatgtc cctgaaatcg aataaaattt
     1501 atttattcct ccatcataca aggctttaac tactcctgct tgataagccc cttttgctcc
     1561 tccaccttct aaacataatc ctaatttcat agtctcatct ccaaattgtt tatcttatat
     1621 ttatatttca aaaaacttac taaatgtttt atatatacta tagtggtcct caacatttcc
     1681 taattctctt atagaatgca ttgctaaaat tggacttccc acatccacag atggtatatc
     1741 tatatgagta gaagatattg gacctatagt acttcctcct ctttcatctg acctattaac
     1801 aaattcttga tactcaacat tagcttctct acatatattt ttataaatag ctatagaata
     1861 tccatcacta gtatacgcct gactagcatt tatctttata accggacctt ttcccattat
     1921 tggtttatta gttgggtcat gcttttctgg aatgtttgga tgaatagcat gagctaaatc
     1981 tgatgatatc ataaaagatg aataaataga ctcaaagaat ccctctctat ccttacccat
     2041 tgatatacaa attctctcca atatatttaa caacatgttt gaatctgcac cttgtttagt
     2101 agagctacca acttcttcat tgtcaaatac agccattaca tttacaccat gttgaccttt
     2161 agtattaaca agagcattca aactagcatg taccattgca agattatctt gtctcccaat
     2221 agatataaat tcatcattag cccctattaa acttcctttt tcaaattcat ataaaaataa
     2281 atcaaaatct ataatatcgt ccatatttac atctaagtta gatgctattt gctttataag
     2341 aaaattatct ttttctaatg tatcatttaa catcccaact aaaggtaaca tatctttttg
     2401 cttattatat gaatgaccat catttatact tctatttaaa tgtattgcta gatttggtat
     2461 tatacaaatt ggctttttaa agtctataag cctttcattt ggttttaata tactatcacc
     2521 ttttaaaact actctaccag ctatcccaag aggtctatcc aaccatgtac ttaatattgc
     2581 tcctccatag cattctgtat ttaacttcaa ataagatttt tcagcttcta tttctgcatt
     2641 tggtttaata ctaaatgtag gagaatctga atgagagcct attattctaa atccatctct
     2701 ttctatatct tctgaattta ctacaaaagc tactatagct gataaattct tagtaacata
     2761 atactttcca ccaactctta gattccatct ttgattcaaa actaattctt caaatccatt
     2821 tttatctaag atttccttag tactcttgac tgcatgaaaa gacgacggac tatcatatat
     2881 gaagtctatt aagtttttag caaagtttat actatccatt cctcatttac ctccattata
     2941 aaatatttag tgttgtatat tctttattta tttttttaat tccctgttgg tcaaaagcta
     3001 tagtaacatc tgtaccaatt atagaaacca ctgtcccaac cccaaattta ggatgatgta
     3061 ccttagaacc aagttttata tcatctggat tagtttcttt attagaatct tttattgtag
     3121 cattaacttt atctgcaact ttagatttgc ttatagtact catatacttt ttagtatatt
     3181 tatctaacat attataactt gctttagaat agcttagttc tttttttact ttgtacaatc
     3241 tttctataca ttcttctggc agttcttcca taaatcttga agctattgca acatttgttt
     3301 taccatataa tgttctcttc tctgtcaatg acatatatag ctcttcttta gctctagtta
     3361 taccaacata acaaagtctt ctctcttctt ctatttgtga atcactcata gattttatag
     3421 ctcttgatat aggaaataac ccttcttcca tacctgttaa aaacacaaca gggaactcta
     3481 aacctttaga tgtatgaatt gtcataagcg aaaccctatc attgtcttct tcatcatttg
     3541 attcagatgt aagtgctata cttgttagga atgtctctaa gtctttattt tcataagtat
     3601 catcattact actttcaaac tccaaagcta tagaaataaa ttctctcaaa ttatcaattc
     3661 tgtcttgagc ttcttcacct ttgcctgtta aatctttttc atttttattt cttatctcta
     3721 caagctcgtc catatatcct gtagtgtcca agactttttc tataagtttg ctaacaggat
     3781 aaacttcttt tatagttcta agagtcccta taatatcaac aaatccactt atacttgccc
     3841 ttgccttagt agatatatct gaatttgttt ctatatcaat taatactgag tatatacttt
     3901 cctgttttaa gttagctcta tcttcaattt tttctatggt tctaagccct atacctcttc
     3961 taggaacatt tataatcctt tttatagata tatcatcttg tggattttga ataactctta
     4021 aatacgccaa caaatccttt atttcttttc tctcatagaa ttttgtacca ccatatatat
     4081 tgtatggaat ttgacttcta tttaaagcgt cctctacagg tcttgcctgt gcatttgctc
     4141 tataaagtac tgcaaaatcc ttataaggtc tattttcttt tctagctatt tgtgcaattg
     4201 aatcagcaat gaaatcagcc tcttctattt cactaccagt taattgtatc tttataagtt
     4261 ctccttcttt tttttcactc caaagctttt ttctttttct ttctatatta ttagatataa
     4321 ctttattagc tgcatctaat atcacttgtg tagacctata attttgttcc aactttacaa
     4381 catgtacatt gtcataatct ttctcaaatt caagtatgtt tcttatatct gcacctctcc
     4441 atccataaat actttggtca tcatctccaa caacacaaat attttgatgt tctcttgcta
     4501 ataattttat caattcatat tgagctttac ttgtatcttg gtactcatct accattatgt
     4561 atctaaatct acttctgtaa tatgctaaca cttcatcatt agccttgaaa agttctacag
     4621 ttttaagtat taaatcatca aagtcaagtg cactatttct ttttaaacgg tcttgatata
     4681 gcgcataaat atctgcaatc ttaaccattc tattatctgc catattgata tctttaaact
     4741 gctttggagt ataaagttta tcttttgctc ctgaaattgc acttataact gcttttggtt
     4801 caaacacttt atcacttaaa tttaattctt ttagacagtc tttaaccaat gtaacttggt
     4861 ctgctgaatc ataaattaca aaacttctat tgtaaccaat cttatttata tcttttctta
     4921 gtatacgaac acagcaagaa tggaatgtac ttatccacat atccttagtt tcaggaccta
     4981 tattttgttc aactctctct ctcatttcat ttgcagcttt gtttgtaaat gttatagcta
     5041 atatatttgg tgcttgtaca cctttatctt ctataagata tgctattctt gttgttaata
     5101 ccttagtttt acctgaacca gcaccagcca gtataagtac tggaccttca gtcttttcta
     5161 ctgcttctct ttgagcagga tttaatgtat ctaaattcat atttctcctc ctgttaacag
     5221 ccataaatat attttaatct aaaatatgaa ttacttcaaa ttataattat gtattatatt
     5281 aatagattat ttccaatata ttacaattaa ataacatttt tccatataat ttatttcatt
     5341 gtttaataaa aaaaataacc ttatgatttg gggaatcata aggcaccttt aaggggtcta
     5401 ggttaagggt tattatatat aaatctattt ttttaaaata ggtttagggc ttaattattt
     5461 atttgtatta agttctcttt ccttccaata cttatacaaa tcttcttctt tcttaaataa
     5521 gggtaaattc atatttctat gtacttttac taaccatggg gattctaggc cagcttgtat
     5581 aatattattt tcatttataa acacattttc tgcttttcct tcatcaatca aaatcccctt
     5641 atccaataca tatatgtaat cacatatttc atacattaaa ttcatatcat gactggatat
     5701 tactattttt atattatttt tatttaatcc ctttattata tctacaatag acctagtact
     5761 tactgggtct aaaccagctg taggttcatc aagtaataca atttcatttt ccattgctat
     5821 aactgatgct attgccactc tttttttctg tccatagcta agaaaatgta ctggcctgtc
     5881 tataaaatct attccattaa cagctactag agctttgttt attctctctt taattgtttt
     5941 ttcatccata ccaatgtttc tcattgcaaa ggctatatca tcatatactt ttgaataaaa
     6001 aatttgtttt tctgggtctt ggaacacaat acctacattt tttctaaggt tgtataaact
     6061 tctcttatca tattttaact tttcttcttt aaaaagaatt tctccagatg taggctttaa
     6121 tatacccatt aaattcataa ataatgttga ttttcctgaa ccatttgaac ctattatacc
     6181 tataacattt ccctttgaaa aatccatatt aatgtttaat agtgcattgg tatttttttc
     6241 atattgatac gttaaattat ttattttaaa catcattatc ccctactatg tgaaatttac
     6301 catcatacaa ttttatgtct aatgatatac acatatcatc ataccttgtc ataagtcttt
     6361 tgtacagcat acttccaaga atacctaagg atttatatga agtttttaaa tttatatatc
     6421 caaatcttag ctcttgtgac ttgtatattt ctgaaaattc ttctaaaaat atgaatataa
     6481 acctatatat taacattgat aactcgacaa aagtgtcagg taaatgaagt tttttaaaaa
     6541 agaatattaa ttgattaaaa ggtgttgtaa gtatacaaaa atacacacaa gtcaaacaag
     6601 acatcgctct aaataacaca tgaatagaca tgtcaataga cgcctttgat attcctacat
     6661 taaaactaaa aaattcaaaa ctatataata aatctgcttt attaaaagat atatttacaa
     6721 gagttattcc tatactaaga aataaaaagt acattggtat tctaagtaat tttaaataat
     6781 tttttaaatc tattccagcc acacaaacaa ccaaaatact cattaaaatc ataattccac
     6841 ttaaaataaa tatattttgt attaccatag aagcaatcag aaaaattact cctatggcta
     6901 cttttttgtt tggatttact ttactcaatc tatttgtata tgcatattta tcaattataa
     6961 gcatgattaa cttctctttc ctttttgaag acctaatatg tatcctatta caccagcccc
     7021 taaagctgct tgtgcagaga ataataaact ttctatttct ccacttggtg gctcatatgg
     7081 actgctaaac catggtttgt aatttggatt tatcttagta atttcacttt cagcttgacc
     7141 atctgctcct ccatattcag cacctgagtt gacaagtaat gggaatataa taagagctac
     7201 tactaataaa agtaataata tgttagtttt tgttttagca ctcattagct ttaactcctt
     7261 tatcattatt ttctagtatg cttttttctc cgttttctgt aattaaatta tatacaatta
     7321 cagtaagtat accttctgct attgctattg gtatttgagt cataaagaaa attcctaaaa
     7381 atttaattgc tgaagccatt accccacctg atgggtctgg gaaagccagt gctaattgta
     7441 ttgaagttat tgtatatgtg gctaagtctc ctattgctgc tgcaagaaat actggaatag
     7501 cattatttcc atcttttttc tttaaagcct taaatattag gaatgatacg attggcccta
     7561 ttatcgccat tgaaaaagca ttagctccta aagtagttat tcctccatgt gctaataaca
     7621 atgcttggaa tattaaaact atagttccaa gaacaaacat tacgcttggt ccaaacatta
     7681 ttgctccaag acctacacca gtaggatgtg aacaacttcc tgttactgaa ggtattttta
     7741 gtgctgataa tacaaacaca aatgctccac ataaagctaa taatactttc tttttagggt
     7801 cttgctttac tattttacct attgatttta atccgaataa gaaaaatggt ataaatataa
     7861 ctccccatgc tatagaccat tttactggta agtaaccttc cattatatgc attgcatttg
     7921 atgtaactgg agttaaaatg attaacataa gtgcagctat aacccctaat ttaatattct
     7981 gtttcatctt agtatccctc tctccctttc taataattaa aacataataa tacatgtctg
     8041 atttatttaa taaaataaaa aagcctaact cgatggttag gcttctaatt ttccacaatc
     8101 taaatacaaa cacataaaac aaacatatct gtataattat tataagaaac tttctccatc
     8161 cccgaagaaa ttactttaat attttaggca ggtctcctga cttagcttca tcctactcct
     8221 cttccttccc atgagatata aaaaatatca tcatagtggc taacaaaagt ttcgtctgca
     8281 tcacagtagc gggggctgta aaggaattta acctttttcc ctattaatct tgcttaaaga
     8341 acctataata ttatatattt aatttttaaa ttcttcttaa attattttct atataaatca
     8401 ttgtatacct tttctgtgaa aatttctaca ttttttataa attttattca tttttatatt
     8461 tttatgtata taaattcttt tttgtaagtt tattaatttc taattatctt ctttaaatac
     8521 attgaaacta catcagaaaa tctagttata tctcttgaat atataaaatc atttccatat
     8581 attaatcttt cagcttcaag ttctgattct tttcctgtaa atactcctaa tactaatata
     8641 ttttgctttc tagcttttct cacttcattg gctgtatctc tcaaaccaac tataccccta
     8701 taagcctttt ctcctcttaa tgttctctca ctactttttc ctatctttac atcatttggt
     8761 cttccatcac tgagaactat caggatttta ttttcttcat ctctatttat tagaccacta
     8821 catatagatt ttattgccaa accatctcta ttattaccag cacaaaaata ttcaaatata
     8881 ttttcactat tttttatact atcatcataa tctttaaatc tttttaatat tgtatagtct
     8941 aaaaaactag aaaatcccat cactctattt ggtatgccaa cttctgttag agcatttgca
     9001 attatgtaag cttgaattgc cacatttccc tgatttctac tttgagagcc acttgcatcc
     9061 aataatatat caataacgta tttccctttt tcattttcta tatctttata aaatatttta
     9121 ttattattac ttctaccaac tctccaagct ctattggcta atatagtacc acaatcagaa
     9181 tatactctac ttttagcatt ttcttctatt aaaattcttg atagactttc ttttaatctc
     9241 tttatttgct gtctatacat tctagcatgt tctctaaatt ttcctaaatt attttctttt
     9301 tgtcttgtta catattttag ttgaaataca ttattacact cacttctaag aactccatct
     9361 gtaaagtgta ttctacaacc ttcatgaaca tttctacaat tttgtgtctc aattttttta
     9421 atttcacctt tactcaaata agtttttcca taataatgtt ctatttttga atatatctta
     9481 tttgcagtgt cttcatcaac atatataact cggtctgctg aattgctaca tttagaatca
     9541 tcaaaatccc ctacaccttc tacaagcatg gatgaagata ttgaatctat atcactttca
     9601 ataatatttt cttcttcatc actataaagt tcttcataca tgaaatcagc aaatgtatca
     9661 aaatttactt ctacactttc tacattattt tcatcaataa gactattttc ttctccattt
     9721 aatatgtatt caaaataagt taaatatata acatctaatg cttttaaaat ctccataata
     9781 tcttgattgg tatctatatt tcttatatca ttaactattc ttctagtcat actatctaat
     9841 gctgggtgct tgtccatact ataaagtaca aatgtgtatt taattttatc cataatatta
     9901 tcattgtgta ttttggtaaa acgtgaaaca atatctttat aagcatttct tcttatgtct
     9961 tctatccctg gcctatcttt aatgaccttt tcttcaacta ttgtatttaa tataacttga
    10021 attagaccca gtataatatc tttgttgtat ccttttctta ttctatttgt aagatatttt
    10081 ttaactatat cccaatctac atactttctt ctagcacctg caattattgc aaaatataga
    10141 cctatttctt ttgaagagta atctttttct gatatatcta tactttcatt gtaatcacca
    10201 gctattgtcc atgataagtt attagctcta ttttcaaatt cataatcatc atatatccat
    10261 ttcataatct cacctatatt aaatatgact tattggaaac acatctatac tctcccattt
    10321 attaggtatt ctagttttaa ttacatctcc aaccatttct ttctcatata catcaaaagt
    10381 ctttcctgta agtcccatat taattgcaag agttggtttt aatcctcttc ttatagtctt
    10441 aagagatgcc ataagaccac gtaaatcaat agctttacta gatatctctc cattttgaga
    10501 cttcaattgt aaatctaaaa atattcctgc aaagtgtatt agtttttctt catcagcatc
    10561 tgaaaattca ttttttaaaa tcatcattaa cttatcttct tctataggag gaatatctat
    10621 taccataaat ctagatacca gtgcttcatt aagttctttt gttcctgcat attcataatt
    10681 catagtccca ataaatcttg ttgctggatg tagattgact ctctcatacc caggtacatc
    10741 aattattctt ctatagtcta aagctgaatg taccacaact atagcatcat tttttgccat
    10801 attaatttca tcaaacaccc caaaacctcc attaatagca cactcataaa ctgagcctct
    10861 tctaagttta acttcattgt ctataaatgt atctgtacct ataagtgttg aactgtctgt
    10921 atttatatga aatgatgtat tccactgagg tctattaaat atttccccta gattatcagc
    10981 aagtaagttt tttcccgttg cctttggtcc cgataataat atattttctt cttctaaaat
    11041 agctgctata cacatagaca agatatcttt accataaaaa taagttttag actttgttac
    11101 tctgtattct aaatcttttt ctaatccata ataatttcta aaatacagta catcttcaat
    11161 aagtttttca tctacccctt gcacccttag attatctatt atatccatat ttttacccct
    11221 tttatataaa tagtatttgt ttcatttctt atcaccattt tagcataaaa ggatataagc
    11281 taaaattgat tttttaatct cccccatttt aagtaaaacc atgcaccatt ttatcactac
    11341 aaaattaaga gtactactta ctgatatttc ttgattttat tcgtctatac gtatataata
    11401 tatactgata aattatgagg aaaggatgat tgaaatatgg aatatatgtc ttgcccagaa
    11461 gtagcaaaaa aatggggtat ttctgaaaga agagtacaaa aactatgtga agaaagtcgc
    11521 ataccaggag tttcaaaact cgggtatatg tggctgatac caaagaacgc agaaaagcct
    11581 gttgatggta gaacaaaaag aagaaaggaa gtacaatatg aatagcgttt tgattataga
    11641 tgatgacaaa gaactttgtg ctcttatgaa aaaatgtgtt gagcaagaaa atttatctgc
    11701 tattactgca catggtggtg cagaaggtct acatttagtc gataaaaata gacacacact
    11761 ctctcttgta attttggaca taatgatgcc agatatagac ggttttcaag ttctgaaaaa
    11821 aattagagaa acaagtaata ttccagtaat tatgcttact gctaaaaatg acgaggatga
    11881 taaagtttcc ggactacgtt taggtgctga cgattaccta acaaaaccgt tcagcataaa
    11941 cgaacttatg gctcgtgtca attctcttat tcgtcggtat actatgctaa accctgcttt
    12001 tataacagac acagatttca taataataaa tggcatgatt attgacaaga caaaccgaat
    12061 tgtttctgtc aataatattc ctgttcaact tacaagcaaa gaatttgatt tacttgcttt
    12121 tctggcttct aataagggac gggtgtttac caaaaaacag atttatacac aagtatggga
    12181 agaagaatat gcttttgacg atagtaacat catgtctttc atcagtaaat tgagaaaaaa
    12241 gattgaacca gaccccaacc atccatttta tattttgaca gttcgtggtg tcggttatcg
    12301 ttttaataag gaggcttaac atgaatacaa gtgtctatct gcttttgata ttggctctta
    12361 gcctgattat tatagcgtac cttgtttctg tcctgctacg tgtccgaata caacttacac
    12421 ttataaaaga tgctttagag gatataaaaa atggaaacct taatcgacgt attttaacag
    12481 atgaaaacga cattacaaaa caaatttgct atgatattaa cgaaatagca atcaatagcc
    12541 aatttcaact tattcagcaa aaacaatcag aacaagccta caaacagcta atgacaagtc
    12601 tctcccatga tgtaaaaact ccacttgctt ctttggtcgg ttatttggaa gcagtagaaa
    12661 gtaaaattgt tgtaggagag gaaaaagacg agtatatcca tgttgcgtct aataaggctc
    12721 attacttaaa gaattttgta gaaaatctat ttgaatgggt aaaattggat tctaaggaac
    12781 aagtttttca ttttgatatt tttgacttga atgaattatc ccgtaatatt atatctgatt
    12841 ggatacctgt tttagaaagc agtcattttg aatatgaatt tgatatacca gaaatagagt
    12901 acttcttacg tatagacgca aatgcatata cccgtatcat taataaccta ttacaaaata
    12961 ttattaccca tagtagtgga aacaaaatga cattgagaat ttttgaaaac aaagagcaag
    13021 ctcaaattat aataacagat aatgggaaag gtatatcttc agacaatctc ccacatattt
    13081 ttgaaaggtt gtaccaatgc gaccattcac gagcttcaaa gggaaatggt ttaggcttgg
    13141 cgattgcaaa ggaacttatt aacgctcata agggaaatat taccgccaac agcactcccg
    13201 gtatgggaac agaatttaca atattgcttc ctaaagccct gtaaaagtac gaggctttct
    13261 tactctaaaa caagataaaa gcaagattat ggcaaggttt tggcaaggtt catgttttat
    13321 aattctaatt gtagttgatt ttgacgttgt atcaactatc ttttgtagaa acaaatttat
    13381 taaaagtgag gaaattatat atgaatgatt ttgtgattga aacaaagcaa cttacaaaaa
    13441 tttatgggaa tcaaacagta gttgacaaag tcaatctcca tgtaaaaaaa ggccgaatat
    13501 atggtctgtt aggacgcaac ggagctggga aaaccacaat tatgaaaatg attttaggat
    13561 tgacttcaat cacgtatggc gaggtagatg tatttggaga gaatattaag ggacgtgaaa
    13621 aacaagtata tccccgtatc ggtgcaatta tcgaaacgcc aggattttat cctaatctga
    13681 caggaacgga gaacttggag atttttgcaa aactgcgtgg tacagccgcc cctaattctg
    13741 taaaaagagc attggaaatt gtaggcttac cttataaaaa taaaaagctg tttagtaaat
    13801 attcacttgg catgaaacag cgacttggta ttgccaacgc tatcctgcat gacccagaac
    13861 tgttagtttt agatgagcca accaatggtc ttgaccctat tggtattgcg gagatgagaa
    13921 agtttattag ggacttgagc gttgagcatg gaaagacaat tcttatttcc agtcatattc
    13981 tttcagaaat ctcattgttg gctgatgata ttggaattat cgaccacggt gttcttttgg
    14041 aagaaagtag tatgagtgaa ctagaaaaga aaaacagtaa atatatcctg ctacaagttt
    14101 cagatattcc gaagacatca cttattttag agcgtcaatt taacacgaag gattattccg
    14161 ttcaagatga ctacacgtta cacctttata acactaatct ggatatgggt gaaatcaata
    14221 aagcacttgt aatgcagaat gtaactgtta ttagttctca actttgtaat gatactttag
    14281 aggactactt taaaaaaatc acaggaggag agggcattgc ttaaacttat tcaaacagaa
    14341 tttcttaaac tgcgtcgtag gaaacttatt tggctgatgt tgttgtctgc tctcattatg
    14401 ccgtttttcg cactactata cttcaattat tttggtaaaa caggtgttga gccaataaag
    14461 ttttataaat ggtctgcatt tggttataca ctttggatta ttttgcctgt tgttttgggt
    14521 atattttgca ccatgcttat acacgaagaa aaccagtatg atatgctcaa acagctttgg
    14581 attgtaccta ttagtaaaat gggatacttt tttagtaaat ttttcgttgt tttgatttac
    14641 tctatttgtt ttatgctgat tacagccgtt gcttctgtgt tatttagtgt actttctgga
    14701 tatgtcgtat ttagctgggc aagcatttta tatttgttga aaaaatgttt agaaattggt
    14761 gtaatcactg cttttgtaat gttgccgatt ttggcaatcg ctacatctca aaaaggatac
    14821 attctcccaa tttgtatcac attggtatat gccttttccg gttttgttat aatgacaata
    14881 aatatgtatt tacacccatt atccagtatg ggagttatta tcatgagaaa taaagacatt
    14941 ccaggtctta tattttcaca ggaaattaat gtcccattag catttctttg tatttgtatt
    15001 tggagtattg tatcagtatt gtttgcaaat attgcactag gaagaagaaa gtgaggtgta
    15061 gataatgtgg aaattacttt gggctgaatg tcaaaaaatt cgtcgttcaa aaattgtttg
    15121 gattacagtt tttgcatcca ttatgatagc agtcatagtt tttgcagggg ggcaggatgt
    15181 atataatgac ccagatttac attatggctt aaagagcgtt tatgacggtt cacggtatat
    15241 agacaatgca ggttggtata tggacgcagt acaaccttgg tcaacttttt ttgttcttcc
    15301 tgctgtcatt gccctattag gaagctatat tatttgtcgt gaagaagagg aggacactat
    15361 taaatccctg cgtcttattc ccataaatga aacaaattta accatagcaa aaatgattat
    15421 tacttttata ttcagtatac ttctttattt gttactcttt actataacat ttttgactga
    15481 atccgtttta catttttccg atttatcgac aaaattggtt ttaagttgca tgaaagagta
    15541 ttttcttgat ggcataggtg tattctttgc aatttctccc attattgcct tggtatcgcg
    15601 catgaaaaaa ggatattggc ttgcattagt attcacagaa gtttattcta ttgctggact
    15661 atttgcgggt atgtcaaatg tcttacaaac cttttttcct attgcggcaa tatttaatat
    15721 ttctggatac tatataacta ctggagaaaa aattatgggg agtatcatga gtttattact
    15781 ttgtgcctgc ctttctgcat ttatacttaa agggctaaaa cacaatgaaa aaaattgaaa
    15841 taggtatatc aaaatgaaaa agataaaaaa actgattatt ctaattgtat tcttgtttct
    15901 ttttggcatg ttacactgtt atactgaatg gaaattcaac aataacccac ctaaaggggt
    15961 aatagaaaga ataaatgagt ggactgataa gattggactg gattagtaac ttatgttaat
    16021 tatagtgaat gtaatattag caagttattg tttcgtattt taatactata actttaaata
    16081 tttggtttta atcttccaaa cgaatataaa aaaactgtca cagaggcaac atatttttga
    16141 atatttataa agtggtagtt ttgattttca aagctaccgc cttttcttct atataagcaa
    16201 acttatcctc aacaaagtgt aaaacatggt gcattttaag aaaaaagtac cacagacaca
    16261 agcaataaaa gaaacgccga gaacctacaa aaacgtagtg cttatgcaga tttacgacgc
    16321 cttctatttt ctcaaccgtc ttaatgacct tgtacaaaag aaatttttct aatatttttt
    16381 ataatcttat cttgtgatgt tttttatgta taaatgactt aataacaaat ccaataaaaa
    16441 ttatggatat tggtataatt agaacgagga atttataatc attataatgt gacattattc
    16501 ttacccaact ctcagaaaaa atatatccta tactgattaa tgaagtattc catatggtta
    16561 ttcccacgat agatgatatt acaaaagtta taatattttg acctgctata cctgctataa
    16621 aagatatgta tgttctacat aaagggatta atctacatac acaaacagaa atagaagagt
    16681 atttattaaa ataatcttga gatgcaaata ttcctttctt tgcctttgga catatctcta
    16741 ttattttatt tatgatagat ttacctcccc aatttcccac tgtataacaa aatatcgaac
    16801 caagtatacc tgcaattata cttaaaatta aaattgtcag aaatccaaaa tgatttcttg
    16861 cagcaattgc tccacataaa ggtaaaacaa cttcgctagg taatggaaaa caagcatact
    16921 caatcataat taatacaaat acagagatta atccatattg taaaataaaa ctattgatta
    16981 cagacactta cttatctatc tcctttctta tttaaaggtt ttaagggtac atgttctaag
    17041 gattctcacc ctatatttaa agaatcaatc tctttaaact attatatgta tttacacaaa
    17101 gcatttataa catactttaa aaatagtacc ttaaaataag aaacttaata aataaaaaaa
    17161 gattacgtgg ctacgtccta ctctcccagg cag
//
LOCUS       DAMJVI010000285        16235 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.088, whole genome shotgun sequence.
ACCESSION   DAMJVI010000285 DAMJVI010000000
VERSION     DAMJVI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16235
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.088"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(120..812)
                     /locus_tag="PC545_003893"
     CDS             complement(120..812)
                     /locus_tag="PC545_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDF2458556.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKVDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATKLAILNATSKELQKIKYTYLKSIEELDKVSHGLIEECYDADLQFHKEIILAS
                     HNPIFIKYYELIPKTVSSNQYFGFKYRNSLKDMISHHNKIMIAFDSKDSYLGYTSMYN
                     HLEDVIQLFQHD"
     gene            complement(838..2025)
                     /locus_tag="PC545_003894"
     CDS             complement(838..2025)
                     /locus_tag="PC545_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDF2458557.1"
                     /translation="MKKISNQKLLLLSIVTFFWFSQYVYIPFQNPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFSPGGNGFLIAN
                     ILSGISSSTWISYMVHYTNFFSTEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKIPEKISIKKYLSICKDKRLVFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVASSGFASTKFCTKYGPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQKSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIQYGYFILAIIAILASILSYTYYKKVDLTLNYDI
                     IQS"
     gene            2361..4238
                     /locus_tag="PC545_003895"
     CDS             2361..4238
                     /locus_tag="PC545_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2458558.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKTEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNNVGKKNESQEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEESVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVVMLPATLFLFGPIGIYIGNVI
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AFGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSSIKVETKDGVIDITSPVEGECIELSEVKDDVFASKAMGEGIAVLP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKVGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            4548..5954
                     /locus_tag="PC545_003896"
     CDS             4548..5954
                     /locus_tag="PC545_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDF2458559.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYHNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGLDFYNKII
                     DKLIECDIEPFVTLYHFDLPFKLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDIYAARMNDNFKVYYMLDMHHYGEYPGYYMKYLEKRNIVPHM
                     EDGDKEILKKAKMDFIAVNYYRTNCAEALPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACKLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEILDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            5982..6830
                     /locus_tag="PC545_003897"
     CDS             5982..6830
                     /locus_tag="PC545_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDF2458560.1"
                     /translation="MYITKVLNNSLLLAKDDNDEEIILMGKGIGHNYKAGSELNEKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLDLPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSREKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            7332..8024
                     /locus_tag="PC545_003898"
     CDS             7332..8024
                     /locus_tag="PC545_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458561.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEQIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKSDNYSKVHGLRAGADDYLTKP
                     FEITEFTARVLSLIRRYTRFNPKNEEPQQLSFKGLLIDLDQHTVTTNGEPMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            8021..8956
                     /locus_tag="PC545_003899"
     CDS             8021..8956
                     /locus_tag="PC545_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2458562.1"
                     /translation="MKMDEQTIILFLLLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGDENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIEQEREEYLEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLNFEIDIPENRLMVNLDPDGYSRVVNNLVQN
                     VLAHSKARQIKITMSEDSRMVLLRVEDDGVGIARENLPHIFERLYKCDKGRSEKGSGL
                     GLSIVSQMVERMGGQVSVESEIGKYTVFTVSLPLI"
     gene            9104..10027
                     /locus_tag="PC545_003900"
     CDS             9104..10027
                     /locus_tag="PC545_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458563.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGAGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRTFIRDLCTERGKTILISSHILSEIALLADDIGIIDHGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLETQLNLKNFKVADNYNIMLYDTDVSVAEINRAF
                     ILNNIEVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            10011..10781
                     /locus_tag="PC545_003901"
     CDS             10011..10781
                     /locus_tag="PC545_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458564.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIAFGIMFFVTVLPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TVLRQPYLLSTPACVGILCLITIIAVLLICMIYKKQEN"
     gene            10791..11573
                     /locus_tag="PC545_003902"
     CDS             10791..11573
                     /locus_tag="PC545_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458565.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQVVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFGITLIGGYLINREYTDQTLKNILTVPISLRKLLVGKL
                     IAVGGITVLLSLFSFLCTFILGLLFCNINISVILVVKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNAFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLVGYNNGAGTVYQPLI
                     GAMTLIVILILTTILLVFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            11950..12486
                     /locus_tag="PC545_003903"
     CDS             11950..12486
                     /locus_tag="PC545_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HDF2458566.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            12518..12907
                     /locus_tag="PC545_003904"
     CDS             12518..12907
                     /locus_tag="PC545_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDF2458567.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLAQLTEHGFIRKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLQELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            12944..13330
                     /locus_tag="PC545_003905"
     CDS             12944..13330
                     /locus_tag="PC545_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HDF2458568.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNNVTVKVSSTTHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            13457..14899
                     /locus_tag="PC545_003906"
     CDS             13457..14899
                     /locus_tag="PC545_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDF2458569.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDEKIAEDIVEDESKLNTEKEGDLNYLSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAQNKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGQDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            15196..16011
                     /locus_tag="PC545_003907"
     CDS             15196..16011
                     /locus_tag="PC545_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2458570.1"
                     /translation="MERLKIQVLVDNNTYIDRYFVGEPAVSYYIEIDGNRILFDSGYS
                     DVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYDLSTVELISHPNCFIPRK
                     HGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIPSINDFEKRAKIGKCKIG
                     DLWEDDYVLDDSAIVCKTDKGIFIVTGCSHSGICNIVEYAKKVCGDDRVIGILGGFHL
                     FELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILNINEVGVGLTISI"
BASE COUNT         5877 a         2007 c         2975 g         5376 t
ORIGIN      
        1 ttgttgaaat gatgaaaata tatttgattt catacaaaaa agagtacctc ataactaaaa
       61 agttcacttt gagacactcc atttttatat gttagttgca tttaaggtaa aatagaagtt
      121 taatcatgtt gaaataactg aattacatct tctaaatgat tgtacattga tgtataacct
      181 aagtaagagt ctttactatc aaatgcaatc attatcttgt tatggtggct aatcatatcc
      241 ttgagagaat ttctatattt aaaaccaaag tattggttag aagaaactgt tttaggtatt
      301 aactcataat attttataaa aattggatta tgagaagcta aaattatctc tttgtgaaat
      361 tgcaagtcag catcataaca ttcttctatc aaaccgtgag aaaccttatc caattcttct
      421 attgacttta ggtaagtata ttttattttt tgaagttctt tagatgtagc atttaaaatt
      481 gctagtttag tagcttcact ttcgataagt tgtcttgctc ttaaagattc tacataaaat
      541 tcttgtatgt tatgattttt atagatttca ccatcatcaa ataggtcaac ttttttaaat
      601 ccttcaacta taggacttga ataaggcaca gctctaagta ttccttcctt acagagtata
      661 tttatggcat ttcttacagg tattctactt atgttaaata ggtcagcaat ctttctctca
      721 cttggtaatt tactattaat tttaaaagtt ccatttagaa tgtttaattt aatataatca
      781 ataatttttt cagtggcatt taagcttttc ataaaaactc ctaaacatat aattttttta
      841 tgattgtatt atatcatagt taagagttaa atctactttt ttataatatg tataagacag
      901 gatagaggcc aaaatagcta taatagccaa gataaagtat ccatattgta ttgaagtata
      961 ttccgatatt ttaccaacta tcattggaaa tacagacatt cctatagcat aaattgcttg
     1021 aaaaaaaccc attgcagtag atttctgatg ttttggtaca tttttcatag actcgctagt
     1081 aaggtaagaa aggagtatac ctgtagatat acctggtagt acttgtagta caaatattat
     1141 attaatattt tttattgttg gaactaaaaa gcaataggct gaaactaaaa taaaaactaa
     1201 aggaatccaa aattttggcc catacttagt acaaaattta gtagaagcaa agccagaaga
     1261 agcaacagca cacaccatgt aaaatataga agatagacct atgagggtag aactagaacc
     1321 taagttttta agaatttgag tagtaaatga cattgttgta gacatttgaa taccctgttg
     1381 aattaatgcc ataaaggaaa aaaatacaag tcttttatcc ttgcagatag ataaatattt
     1441 tttaattgat attttttcag gtattttttt tgaattatga ttttttatat ttaaggctag
     1501 aatcgttcca ataactgaag acaatacact catgatacat aaggctgtca ttcctaattt
     1561 ttcataaaat aaagtactca taacaaatcc aatcaacatt cctagattgt ttactaatat
     1621 tattgtactt gtagcttttt gttgatgttc tgtagaaaaa aagttggtat agtgaaccat
     1681 gtaagaaatc caagttgatg atgatattcc agataaaatg ttagctataa gaaaaccatt
     1741 gcctccagga gaaaagactc ttataagaga tgctaaacca gcaaagaaaa ttccagacag
     1801 tataaatatt ttatgtttat ttatggaatc agcaaatact ccaacaggaa gccttaataa
     1861 acattgtgag attccatatg cacctattat tataccaatc atatttgtag atacattaca
     1921 tgcacttaaa tatggatttt ggaaaggtat atatacatat tgtgaaaacc agaaaaatgt
     1981 aacaatagaa agtaaaagta atttttgatt agatattttt ttcatattat cctccgtatg
     2041 gtatttttat aatgcaaata atataccatt tggacttttt aatcaagtac tttctctatt
     2101 aaaaaaaatt taaatatata attattattt acaaaaaaat tatactttat agttgaatta
     2161 agaaaacgct tatgatacta taaagttaac aaatttgaat atataacagt agacgtgtta
     2221 ctggtaatgc aggcaagatc gaatcaattt ttatagatta tctatagagt actatgggta
     2281 taatctattt atttggttcg atttttttgt ttaattggcc ataaaacaaa aagcctatag
     2341 ttgaaaatgg aggatttttt atggattata aaaatatagc tcaagaaatt ctactaaatg
     2401 taggaggaaa agagaatgta aatgaagtga ctcattgtat gacaagactt agatttaaag
     2461 tcaaaagcgc ttctaaagtg aataaggata aattatctaa aactgaagga gtaataactg
     2521 ttgttgagag tatgggtcaa atacaagttg taattggaaa taaagtaaaa aaagtatatg
     2581 atgaggttat aaaaattgta cctcaatcaa ataacgtagg taaaaagaat gaaagtcaag
     2641 aaaagcaagg tataattaat tctatattat cagcagtagc tggtatattt acacctacaa
     2701 tacctgctat agcaggggtt ggtatgatta aaggtatttt atcagttctt gctatgtatt
     2761 atatgaataa aaatggagta gatataaaag aaacgcaaag ctatattatc ttaaatgcca
     2821 tggctgattc tatattctat tttatgccta ttatactagg atatactgcg gctaaagtat
     2881 ttaatgcaaa taaaataata tctatggtat taggagctac actttgttat ccaactttta
     2941 ctgctttgat ggcaggtgaa gaatctgtta gatttttagg attggcagta acaaaagcta
     3001 cgtatacatc atctgttatt ccaataatta ttgctatatg ggctctttca tacgttgaaa
     3061 aagtattaga aaagtatata cctgaggtaa ttaaaataat aatggttcca actttatcat
     3121 tggtagtaat gttacctgca acattatttt tatttggacc aatcggaatt tatataggaa
     3181 atgtcataaa cttttcttat aagtatattt atgaattaag cccagcattg tgtggagcat
     3241 ttgtaggtgg gttatggtgt gtgttggtta tatttggtgc tcatagagca ttattaccaa
     3301 taggtataag tgatgttgct caaacaggaa gacagaattt attggcattt gcaggagctg
     3361 caaacttttc acaagcaggg gctgctctag gagtattctt taaaactaaa aatcaaggac
     3421 taaaaacaat ttctatgtct gcaactataa cagctttatt tggtataact gaacctgcaa
     3481 tatacggtgc caatcttaga ttaaaaaagc ctatggtatg tgccgtaata tgtggtgcat
     3541 ttggaggtgc gataatggga atgggtggag cttatggtaa tgcatttgca aaccaaggtg
     3601 ttttaactat acctgtatat gctgaagcag gtgcattagg tttcttaagt tatctaggtg
     3661 gttgtgctat agcatttttt ggttcagcta tttcaactta tctagttgga tttgaagatt
     3721 tggaagagtc ttctaataag gaaaattcat ctataaaagt agaaacgaag gatggtgtta
     3781 ttgatataac ttcacctgta gagggcgaat gtatagaatt gtcagaagta aaggatgatg
     3841 tatttgcttc aaaagctatg ggagaaggta tagctgtgtt acctacaaaa ggagtaataa
     3901 cagcccctac tgattgtgaa gtagcttctt tatttccaac attacatgca ataggattaa
     3961 aattagataa tggagcagaa atgttaatac atgttggaat caatacagta gagttaaatg
     4021 gtaaatactt taccaaacat gttaatcaag gagatttagt gaaaaaaggt gataaactta
     4081 tatcgtttga tatagataaa ataaaaaagg ttggttatga tgtaactacg ccagttatag
     4141 taaacaatac atttgatttt gggcaagtag tttcttgtaa atctagttat gtttcaacca
     4201 atgataatat aatttcatta gtattagctg gtaattaagt taatttgact agagataagt
     4261 aaattgattt aaaaggaaat aagtaaatca acaattaaat acatctaaaa gtaagtttat
     4321 tgcaatgaat tgttaaagaa aataattaat atggagtaaa tctattgcaa tgaagtaaca
     4381 aagaaaataa ttaatataga gtaagtttat tgtaatgaat tgttaaagaa aataattaat
     4441 atagggtaaa tctatttcaa tgaaataaca aagaaaagag ttaatataga gtgaacttat
     4501 tgcaatgaat caacaaagaa aataattaat aaaaaggaga aatatttatg aatacaggat
     4561 ttccaaaaga ctttttatgg ggagcatcat cttctgcatt tcaagtggaa ggagcttggg
     4621 ataaagacaa taaaggaaaa accgtagccg actataattc ttttaaaaag tctcatttac
     4681 aggcagatac taaagtcgca tcagactttt accataatta tgaagaagat attgaattaa
     4741 tgaaagaatt agggatgaaa acatatcgtt tttcaatttc atgggcaagg ataataccag
     4801 atggtgaagg agagataaac cagaaaggtt tagactttta taataagata atagacaaac
     4861 taatagagtg tgatattgag ccattcgtga ctttatacca ttttgacctt ccatttaaat
     4921 tagtagagaa atataatggt tgggaaagta gagaaactgt ctatgctttt gaaagatttg
     4981 ctaaaatatg ctttaagcat tttggagata gggtaaagta ttggcaacct cataatgaac
     5041 agaatttaat tgttagagtg gaggagagaa ttaatatata tgatgaaaca gattcatgga
     5101 agatagataa aatgcgtgct cagatggatt ataatctttg cttagcacat gcattagcag
     5161 tcaatgcatg tcatgagatg ataaaagaaa gtaaaattgg agcagcagta tcatcatctg
     5221 taacatatcc tcttacttct aagccagaag atatatatgc agctagaatg aatgataatt
     5281 ttaaagtgta ctatatgctt gatatgcatc attatggaga atatccaggg tattatatga
     5341 agtatcttga aaaaagaaat atagttcctc acatggaaga tggggataaa gagatactta
     5401 aaaaagctaa aatggatttt atagctgtaa attattatag gacaaattgt gcagaagctt
     5461 tacctgaaga ttcacaacat ccatttggat taagagaagg aactgttgat tttagtatgt
     5521 atggactatt taaaatgtca atgaatccta acttagaggc ttctgagtat ggagcagcaa
     5581 tagacccatc aggtcttaga gttgccttaa atgaatattg gcaaagatat cacttacctg
     5641 ttataataac agaaaatggt ctaggagcta aagatatatt agaagatgga aaaattcatg
     5701 atgactatcg tatagactat ttaagaagtc atataaatgc atgtaaacta gctattgaag
     5761 atggtgtaga gatgattgga tattgtccat ggtcatttac agacctttta agttcttctc
     5821 aaggttttaa taagcgttat ggacttgttt atataaatag aactgaccat gaaattttag
     5881 atttaaaaag aatcaagaaa gatagttttt actggtataa agaagttata gagaacaatg
     5941 gaatagtaaa ataaacataa atatatattg agggttttag tatgtacatt acaaaggtat
     6001 taaataattc gttgctactt gcaaaggatg ataatgatga agaaataata ttgatgggga
     6061 aaggcattgg ccataattat aaggccgggt ctgagttaaa tgaaaaagat atagaaaaga
     6121 tttttgttct tcatgatgaa aatataaaaa agagtattat acaattggca acagaaatag
     6181 atgcagaata ttttggtata gctcaaatga taattgcata tggaatagaa aaatataact
     6241 taaaacttat gaaccatata tatttggcct taactgacca cattgccttt gcagtgagac
     6301 gttttaaaga aggtataatg attgaaaatc actatttgtt cgaaatcaag gaatttaatc
     6361 caaaagagta tgatatagga aaatatgcta ttactgtttt taaggaggtt cttggtttag
     6421 acctaccaga agaagagatt ggtaatatag caggccattt tataaatgca caacaagata
     6481 atccttatag tgatagaaat aagagaagtg ctaaaattgt aaatgctatt ttgcaaatag
     6541 ttcattatca cttttctatt gtatataata aagagtcatt ttattatagg agatttgtaa
     6601 tgcatctaaa ggccttttca caaagatttc tggctaatga accatcaaga gaaaaaatag
     6661 attttatata tgagcaagtt caaaaaaatt gtaaggaaga gtatgaatgt gtgaaaaagg
     6721 taggagctta tatgtataag gaatactcca gagaattacc aagacaagag gagttatatt
     6781 tgatgattca tattcataag attttaggag aattatgcga aaatagttag aatatcttaa
     6841 tatgctagta atattataaa aaattaaaaa ttaactgcac tttaaatatg aataattgca
     6901 ttacttaagg gggttgtctt aaaattgatt tttttaatta aatttgaagc tcaactcctt
     6961 ttttaaacct ttatatcatt acaactttaa tgtttatacg cctgtacttc agatgtatat
     7021 ctacttttta catttctcaa catgcaaatc taccattctg tatatagtaa atgtattatc
     7081 atgtttgttt tattcaagca cacttataat acgcttgatt ttttcacaat gatgaaatat
     7141 actaatgggt gttataggta taagaatcgc attacacttg aagaagtaag tgataaatga
     7201 gatataaatc ctcatatgat aaactactat ttcattgagg aacatattct aagtgatgtc
     7261 aagatactac aatatgacta aattcctagt gataaaaaag tctacgaatg agtgtagaaa
     7321 ggggaactaa gatgaataaa gttttaatta tagatgatga taaagatctt tgtattttgt
     7381 taaaaaagag tatttcaatt gaacagattg aagcaaacta ttgccattct gggagagatg
     7441 gactggttga actttcaaaa caagattatc agttagtagt tctagatgtg atgatgccag
     7501 gtatggatgg ttttgaaact atggaacaaa tacgaaaata tagcagtatt cctatcttaa
     7561 tgctgacatc aaaaagtgat aattacagca aagtgcatgg attgcgagct ggtgcagatg
     7621 actatctaac caaaccattt gaaattacag aatttactgc tcgtgtactg tctttaatcc
     7681 gacgttacac ccgttttaat ccaaaaaatg aggagccaca gcaactttct ttcaaaggtt
     7741 tgttgattga ccttgaccag catacagtta ctaccaatgg cgaacctatg ttgcttccac
     7801 ccaaggaatt tggtgtactt ctttactgtg caaggcatca gggtaagatt ttgacaaaac
     7861 agcagattta tgaagccgtg tggggtgagc catatgtata tgatgacagc aatatcatgg
     7921 ctattatcag tcgcctaaga aaaaaaattg aactagaccc atcatctccc atctatttgc
     7981 aaacaataaa aggaattggg tatcaattca acacggaggt atgaaaatgg atgaacaaac
     8041 gataattctt ttcttactct tgctgtcgat tggtctactt tttctttcca tatttgcttt
     8101 ttccaagctt gggcatattc acaaacgctt aaaagatata gaggagatac tcgctgatgt
     8161 acaaattgga gacgaaaatc gtaaaatact aattaagcca tgtgatgtta tggctccatt
     8221 ggtttatcag cttaatgaga ttgtctacga ttatgaaaat aagttacttt ctctgaaaaa
     8281 gtcggataaa gctagcaaac aacttatgac aagtttatct catgatgttc gtacaccatt
     8341 aacaactctg attggttatc tggatgctgt acacagtggt atagttatcg aacaagagcg
     8401 ggaggaatac cttgaaatcg ctaggcgtag agcctacgac ctcaaggatt acattgatgt
     8461 tctttttgac tggtttcggt tgaactctga tgaatttacg ctgtcaatag aatctgtgga
     8521 aattgcagag ttgtcacgca atattttgaa agactggatt cctatattcc atgaaaagaa
     8581 actgaatttt gaaattgata ttccagaaaa tcgactaatg gtaaacctcg acccagatgg
     8641 atattcccgt gtggtcaata atctggttca aaatgttctt gctcacagta aggctcggca
     8701 aatcaagatt accatgagtg aggattccag aatggtatta ctccgtgttg aggatgatgg
     8761 tgttgggatt gccagagaaa atttaccaca tatttttgag cgactgtata agtgtgataa
     8821 aggacgttca gaaaaaggaa gtggtcttgg tctatctatt gtcagtcaaa tggtggagcg
     8881 aatgggtggt caggtttccg tagaaagtga gattggaaag tataccgtat ttacagtgag
     8941 cttaccatta atataaaatt gtttttcccc catgtgacat gtatacacag ggggattttt
     9001 tgtaagttat aaagttgcaa ggttaataca aggttgttgc aaggttaatg taagattgag
     9061 gtggtatgat aactttaata aacaaaggag gcttttatta tttatgaata atttaattat
     9121 tgaaacaaaa aatctcacta agcaatatgg tgctcaaaag agcgtttcta atttgaatat
     9181 tcacgttcag aaaggtcgca tttacggatt gctaggaaga aacggtgcgg gtaagacgac
     9241 aactatgaaa atgttattgg gcctaacaaa accaactact ggagaaatta aaatatttgg
     9301 tcaggacatt cacaataacg agaagaagct tcttcctcga attggatgtt tgattgaatc
     9361 accagggttt tatcctaatc tgacaggaac agaaaatctc aaaatctttg caaggcttag
     9421 aggaatacca aaaaaaaatg cagtagaatc agcactggat gtagtcggtt tgccaaacaa
     9481 tgacaaaaag ttattttctc aatactctct tggtatgaaa cagaggttgg cgattgctct
     9541 tgccattatg catgacccac aactgttgat tttggacgag cctatcaatg gacttgatcc
     9601 tattggtatt gcagaagtaa gaacgtttat tcgtgatctc tgcacagaac gaggtaaaac
     9661 tattttgatt tccagtcaca ttctttctga aattgccttg ttggcagatg acattggcat
     9721 tattgaccat ggtattttgt tggaggaaga aagtcttgcc caattagaag aaaaaaacag
     9781 taaatacata cattttgctg tatctgatgc agcacaggca gcaaggcttc tggaaaccca
     9841 acttaacctg aaaaatttca aagtagcaga taattacaat attatgctat atgatacaga
     9901 tgtttctgtt gcagaaataa atcgtgcttt cattctaaac aacattgagg tatcagaatc
     9961 tcatttatgc gaagatactc ttgaagatta cttcaagaaa gtaacagggg gtgaaggaat
    10021 tgcttgatat aatttcatgt gaattttcaa aactcaagcg gttaaaattt atactgattt
    10081 ctattttagg tgcttgctta tttccaattc ccgcaacaat tttgattgca aaggacaact
    10141 tgccctttga gcaattgttc aaattagttg tcaattttgg atatttcttg ctattaccaa
    10201 ttgtattaag tattgttgct agccaactat tttttataga aagagataat gatgtattaa
    10261 aaaatcttgc aacagtgcca gtgccaaaag gaaaactggc tctggcaaaa ttggctgtgt
    10321 tgctgtttat atcattgttc tactctgttg cagggttagg agctactatc attggaggtt
    10381 tcattgttgg aatagtagag ggggttgctg taaaattagg aatgagcatt gcttttggaa
    10441 ttatgttttt tgttactgta cttcctgttg tggtactcat tgtatatttc aatcgcagct
    10501 atattttttc tattatactt tcttttgtat atgctatttt caattttagt atatccttaa
    10561 atattatcaa ttttgaacca aataacccac tattaagcgt attacctgct cctgttataa
    10621 tgcgttggtg gatggcattt tggggagatc ccactggcga atacacggta cttagacagc
    10681 catatttgct ctcaactcct gcttgtgtag gtattttatg tttgataacc attatagcag
    10741 tcctgctgat ttgtatgata tacaaaaagc aggagaatta ggaggtatct atgcttgata
    10801 taattactac agaatttcaa aaaataaaaa gatataatat tctatggatt ggtgttgttg
    10861 ctgttctgtt ttcagcacta ttggcagttt ttcaggttgt aagttcacac ggcagtgacc
    10921 ctctaacgta tgagagcttt gcaaacggag ttatctggaa taatttcagt cttagttttc
    10981 catttgggat tacacttatt ggaggatatc ttattaaccg tgaatataca gaccaaacct
    11041 taaaaaacat cctaactgtt cctatttcat tacgcaaatt gctagtggga aaattaattg
    11101 cggttggagg aattactgta ttgttaagtc ttttcagctt cttatgcaca tttattttgg
    11161 ggttgttatt ttgcaatatc aatatatctg ttatactcgt tgtaaaatcc ttagttcaga
    11221 ttttgtctgt taatctgtat tgttatgttg ctgttttgcc gattattgct tattttggtc
    11281 gtaaacaaaa tgcatttctg acgggtgtag ggattgcatt tgtatacggt ttttgtggtg
    11341 tgtttgttgc tggaagaaat ctgacagact tttatccaat cacagcaggt cttggtctag
    11401 ttggatacaa caatggtgct ggtactgttt atcagccact tatcggtgct atgactttga
    11461 ttgtaattct aatcttgaca actattctac ttgtatttac acctaattat gataaagtta
    11521 tgaccatatc taagaaaaaa gggataaagc ataaaaagta ttctcataaa taacataaat
    11581 attgtttcta ttatttaagt tgctagacag tgaaaagaaa aaatctgtcg tacagtggct
    11641 atatgctcat gtattcaaga agctacagtt ttgatttttc ataactgtag cttctttttg
    11701 tatttattaa atcacgacta tcataatact tgcaataggt atagtaacaa gagagagtac
    11761 tgtcatatgt gttttatata ttacattaag tatattctaa aatatatgtt ttaaataata
    11821 tatttgaaaa taggaatatt taaataaaaa aaataatatt atatatagtt gacaaaaatt
    11881 ggcaaatgat atactatata aatatagaaa tagtaatcat tactattatt aaaaagggag
    11941 gaattaatta tgaacttaaa aggaactaaa acagaaaaga atttaatggc aggatttgca
    12001 ggagagtcag aagcaagaaa taaatataca tactacgctt caaaagcaag aaaagaaggt
    12061 tataatcaaa tagctgctat atttgaagaa actgctaaca atgaaaagga acatgctaaa
    12121 ttatggttta aacttttaca tgatggtatg ccttcaacag aggaaaattt aaaggatgca
    12181 gcagcaggtg aaaactatga atggactgat atgtatgcaa aatttgctaa agaagctaga
    12241 gaagaaggat ttgacaagat agcttattta tttgaagctg ttggaaaaat agagaaagag
    12301 catgaagaaa gatacttaaa gcttttagaa aacttaaatg aaggaaaaat atttaaaaga
    12361 gatgaagaag ttgtttggca atgtcaaaac tgtggtcatg tgtatgtagg tactgaagca
    12421 ccagaaaaat gcccagtatg tgaccatcca aaagcatatt ttaatataaa agctgaaaat
    12481 tattaaaaat tatcattatt aaaaaggaat gttaactatg aaattttcta aacaacgaga
    12541 actgatttta aatgaaatat taaataatcc ggttcatcct actgcggatt acctatatga
    12601 aaacttaaaa aaagataatc cgaatttaag cttaggaact gtgtatagaa atttagctca
    12661 gctaacggaa catggcttta taagaaaggt tagtattcca gggtatccag atagatttga
    12721 tggtcgaata gataatcact atcatatcat atgtgaagta tgtggagagg tatacgattt
    12781 agaatccgaa gttcttaata atttacaaga attaatatct gaggagacag acataaaaat
    12841 aacatcttat aacataagtt ttaaagggat ttgtaataat tgtaaaaggt gtagccaagt
    12901 aggttagttt tcaaattcta aaaataatta atgaggtgag tttatgtgta gtgaacaaaa
    12961 attttttata tgtgagactt gtggaaacct tgtaggaatg atacaaagtg gtggtgtacc
    13021 tattttctgt tgtggaaaac ctatgaagga acttgttcct aatacaacag atgcagcagt
    13081 tgaaaaacat gtacctgtaa ttgaagtgga tggaaataat gttactgtta aagtaagtag
    13141 cacaactcat ccaatgacta aagaacatca tatagcatgg gtgtatctta tgactgaaca
    13201 aggtggtcaa cgcaaatgtc tagcagtaga tggagaacct gtagttaaat ttgctttgaa
    13261 tgatgatgat aaagtgattt ctgcctatgc ttactgtaat ttacatggat tatggaaggc
    13321 tgaactgtaa tatttaaata aattgagtca aaaaggaggc tataaatgta ttttcataca
    13381 aatagcctcc tataaattta tataaattca agttttaatt ttgtttaatg tcaatataat
    13441 ataaggagaa aataatatgt caatttataa atgtagtgtt tgtggatata tttatgatga
    13501 atctaagaat gataaaacat gggatgaatt aagtgaagat tgggaatgcc ctgtatgtac
    13561 aaaaggtcgt agttattttg gaaagataag tactgtttac tatgaagaag atgaaaagat
    13621 agcagaagac atagttgaag atgaatctaa attaaatact gaaaaagagg gagatttaaa
    13681 ttacttaagt acatatttaa gaagagatga tgaagttgag aaacatatgg atataattca
    13741 cgaaatggct gttacaggaa aatctataat agaacctatg agaactaaac taccagtaat
    13801 ttcttgggat gatattttaa ttatgggtgc tcaattaaat ccattacctt taaatgagca
    13861 tgatgaagta aatacaacaa caataatagg aaaaaaagca aagaaaccta tgattataga
    13921 aaatccagtt tatatttccc acatgtcatt tggagctttg tctaaagaac taaaaatagc
    13981 tttagcaaag ggtgcagccc aaaataagac agctatgtgt agtggtgaag gaggtatatt
    14041 accagaagag aaggaagctt catacaagta tatttttgag tatgtaccaa ataaatatag
    14101 tgtaactgaa gaaaatctta aaaattctga tgctatagag ataaaaattg gtcaaggaac
    14161 aaaacctggt atgggaggtc atcttcctgg cgaaaaagta actgaagaaa tagcaaaagt
    14221 aagaaataag cctgtaggtc aagatgtaat aagtccatct tgttttgagg aaatacaatc
    14281 caaagaggat ttaaagaaac ttgtagatga acttagagaa gtttcagaag ggcgtccaat
    14341 aggtgttaaa atttcagcag gtcatattga aaaagatatg gaatttatag cttatgctaa
    14401 acctgatttt gtaactattg atggtcgtgg tggagctact ggagcaagtc caaagttgct
    14461 taaagatgca acttcaattc caacaatatt tgctctttat cgtgctagaa aatacataga
    14521 tacacatgga ttagatatag atttggttat aacaggtggg cttcgtatat ctactgattt
    14581 tgctaaggca attgctatgg gggctgatgc agttgctatt gcaagttctg ctcttatggc
    14641 agcggcgtgt caacaatatc gtatatgtgg ttctggtaag tgtcctgtag gagtagctac
    14701 tcaggatgag gaattgagaa aaagattaca tatagaaaat agtgctaata gagtggcaaa
    14761 ctttttaaat gtaagtttag aagaacttaa gacttttgct agaatttctg gtcataaaga
    14821 tatacatgat ttaagtgttg atgatttata tactgttaac tctgaaatat caaattatac
    14881 aaacatacag catgtataga ttgaagtttg aaactattag attttagaca gctataaaag
    14941 taaatgataa atatatttta taaaagagag tgtcttaaaa tgaacttttt agttcataag
    15001 tcactctttt ttgtatgaag ccaaatatat ttatcatttt agcaatgaaa aagaatctta
    15061 tctctttatt ttgagataga ctccttctat ttatgaaaat taaagatatt aaattgtaaa
    15121 atattctgtg atgtcgtact attaaactaa agattctgta ctttaaagta aataaaaatt
    15181 taaggagtga ttattatgga aagattgaag atacaagttt tagtcgacaa taatacttat
    15241 attgatagat actttgttgg agaaccagca gtaagctatt atattgagat tgatggaaat
    15301 aggattttgt ttgactcagg gtattcagat gtattcatat caaacgctga aaaattaaat
    15361 atagatttag gaaatttaac tcatgttgtt ttttctcatg ggcataatga ccatacaaga
    15421 ggtatccagt ttttagaaaa taggtatgat ttatctacag ttgaacttat ttcacatcct
    15481 aactgtttta tacctagaaa acatggagag aaaagcatag gagctccatt ttctgctgag
    15541 gaaattaaaa atatttttat gtataatcct aaggataagc catttaatct aagcaagaat
    15601 tgtgtgtttt taggtgaaat accttctata aatgattttg aaaaaagagc taaaattggt
    15661 aaatgtaaaa taggagattt gtgggaagat gattatgttt tagatgattc tgctatagtt
    15721 tgtaaaactg ataaaggtat ttttattgtt acaggatgtt ctcatagtgg aatttgtaat
    15781 atagtagagt atgctaaaaa ggtatgtgga gatgatagag ttattggtat actaggtggt
    15841 tttcatcttt ttgaattgaa taataggtta gatagtacaa tacaatattt ggataaagaa
    15901 gaaataagaa tgttgtaccc ttgtcactgt gtttctttta aggtaaaagc gaagatgaat
    15961 gaaatattga atataaatga agttggagta gggctgacca taagtatata gtatttgaaa
    16021 gtatataaga tttaagttga aggttaaaaa gatattttct atagaaaata tcttttttgt
    16081 tacgtttatt ttaagagaaa tcaatatact tttatatgaa atattgtaaa aaaatttatt
    16141 tttaatgaga atatattttc attaataaac aattttatta tgtattaata atgttagaat
    16201 aaatataaat aaatcaaagt tacttacatt acagt
//
LOCUS       DAMJVI010000286        15199 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.092, whole genome shotgun sequence.
ACCESSION   DAMJVI010000286 DAMJVI010000000
VERSION     DAMJVI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.092"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            278..1231
                     /gene="blaCDD"
                     /locus_tag="PC545_003908"
     CDS             278..1231
                     /gene="blaCDD"
                     /locus_tag="PC545_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HDF2458571.1"
                     /translation="MKRKKNFIWIAILLVGVVMVMYYVGKKHNDINQKTDKNYIKSEL
                     NKSELNKSKIENNDKNKKEKINMVDYSDCFEGISGGAIFYNTKNKEYNIYNKELIETR
                     RSPCSTFKIVSTLIGLEKGVINSKESVMGYDGTEYPNKNWNKNLSLEEAFKESCVWYY
                     KKLIDKVDAKSVQNILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQ
                     TMAKIFEGDTNFKKEHINILRDIMKIDINDKNINVYGKTGTGFDEKNKRVDAWFVGML
                     EREGDTYYFAIKSDDSNKEITGPKVKEIAINIIKKYYSVRE"
     gene            1575..2258
                     /locus_tag="PC545_003909"
     CDS             1575..2258
                     /locus_tag="PC545_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDF2458572.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHLLGGVDRPTSGKIYVEDTEISSLKEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     AILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            2255..4915
                     /locus_tag="PC545_003910"
     CDS             2255..4915
                     /locus_tag="PC545_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDF2458573.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDYTYGSDLPLIENFSSVDDVMVKGQWYVGKINDEKRDYI
                     IYRNATSEYWNSMPEKDTIIEGRRPNNANEIALSKQYFENNPSVKIGDKITLPIGYRI
                     LNGKPLEPIAPKSKGESFKKISEATLTIVGKVDVSTSSVIPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SSISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNRLIVYVNSVNYHTDVPEVVFTYGIPAFLPAVLL
                     TIVTVWISALIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTCFLHIIANQEGAKAVYGSDNYKDQRDIAVYLENSEPIE
                     SAFIEKLDNTEGVKSSLYYQKMSASTWLTEKDASKELAKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDKSFTEYCRQISVDPKLFYNTKNPTSIVYNKEEDINRSTRRNKVY
                     IDYLNLSIGDNIKLNEKVYDEDKGDYTYNMKVGAITSTLPKIVDTSSKYTLMQIVPMS
                     MVQEISKNYDEVSRLRSNRLIGVFKVNNREDIPRVREKLESIYNKDYGSGDYSIEDVL
                     ESEKQKASGIKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPE
                     GIKKMLILEGLFLGITPLLLSIPVQIGIVYAFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYKQLKNENIVESIKNETI"
     gene            complement(5096..6277)
                     /locus_tag="PC545_003911"
     CDS             complement(5096..6277)
                     /locus_tag="PC545_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDF2458574.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKAL
                     GASYEYNPKSTESILKSLKNSSDKDFKTGNEILSKHGYSKSTFWSKNFFYFLVISFGI
                     ISTMATVIYIIKHRTNKQKLSRINNLTQYLEEVNLGKESVLLRCEDEFSHLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TNQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     EASPSVEFEIDINWTLEALLNILKNCSEHIEDGGYIVAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6277..6939)
                     /locus_tag="PC545_003912"
     CDS             complement(6277..6939)
                     /locus_tag="PC545_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDF2458575.1"
                     /translation="MAKILLLEDDDTITFGIKTSLEKKNHTVCCYNTIAEAKKNFDKT
                     IDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRTHSNTNNTITCENISLNKVEVKAYLDNVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(7071..7709)
                     /locus_tag="PC545_003913"
     CDS             complement(7071..7709)
                     /locus_tag="PC545_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDF2458576.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFCLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSEKSDTDMLFKEKFFSFNKFMFDEIQGMFEIGKNDRSIRHDI
                     EIPTLMYSSIFTLTGFFNLLSVTGKSYLNNFNIDEEKFIETTLALLIDSIKA"
     gene            7888..8493
                     /locus_tag="PC545_003914"
     CDS             7888..8493
                     /locus_tag="PC545_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDF2458577.1"
                     /translation="MKIKENVLMLDSTRGSNCFIVFDKEIILIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHISNIKLLKEKTGAKVWAHVEDIPFIIGEKDRPGFKK
                     FIGKAISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEAVLFAGDLVKNKNG
                     NIVPYPNPWNWDYKKMVKSIRELDNLKYEWICMSHGTPCIK"
     gene            8997..9746
                     /locus_tag="PC545_003915"
     CDS             8997..9746
                     /locus_tag="PC545_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2458578.1"
                     /translation="MRLESLVNQNYDNLNENDKIIIKQILICQREYRNFSCEKIANEC
                     NVSRATLLRLCRKIGLDSFSDLKYLLKNNQLEFVNNDNNFHDVCENYRLLMNELQKIS
                     FQDICKIIYDAETIYIYGTGNEQKSLAQEIKRIFLSVGKCVIDLFDIGEIEFMRESFQ
                     KNDLFIIISLSGETKAGIEIAKFINNHIHTISLTRLDNNTIATLCEYNLYVETQKLDT
                     LQAISYEVVGAFYALLDLLHMNYIEYRRRKE"
     gene            9743..10507
                     /locus_tag="PC545_003916"
     CDS             9743..10507
                     /locus_tag="PC545_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDF2458579.1"
                     /translation="MKIEGLFNKYYNSFTANDKYICECILNHKEDCIKLSIDEFADKY
                     HISTSALSRFAQKLQLPGYSELRVIIRLNETEVEYKNQSKHQLMDCYNKVIDDIEKKD
                     CSIMFERMKKANRIIVFGEGYSQERVAKEMKRIFLPTGKIIYDVYGYDMIQALNHFIK
                     TDDMIFFISLNGDASTIIDFAKEMRMKGIYMVSITKMISNPLARLCDENLYIQSISIS
                     VDKQLDYDVTTPYFILIELLYIKYKMYLESEHIPRK"
     gene            10606..11427
                     /locus_tag="PC545_003917"
     CDS             10606..11427
                     /locus_tag="PC545_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDF2458580.1"
                     /translation="MKLYKLLILDIDGTLRDEVYGIPESAKHAIRLCQKNHCSVVICT
                     GRSMGTIQDDVLSLGVDGYIAGGGNYIQYHGELLYNQSFNQRLIKEVVCLLKKREVAF
                     SIESQEKVFMNQKAKEIFETMNQLKGTNSCINKQHIQEKITYENNIEEYKSQDIHKIC
                     LWSNEKVFDEVKNILQDKMELAQRDISSQYYEIIQKDFHKGKAIKRLQERLGVTQKET
                     ICFGDGQNDVVMFQASDVTIAMKNSHQQLKDIATSICEDIFDNGIYKELKRRNII"
     gene            11441..13105
                     /locus_tag="PC545_003918"
     CDS             11441..13105
                     /locus_tag="PC545_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDF2458581.1"
                     /translation="MEKKWWQKEIVYQIYPRSFQDSNNDGIGDLQGIISRLDYLKNLG
                     ITSLWLCPIFKSPMDDNGYDISDYLDIASEFGSMEDLKELIKESKDRGIKIILDLVLN
                     HTSDEHPWFQEALKNPESPYHDYYIFKKGNDLPNNWRSVFGGSVWEKVENRDEYYFHS
                     FGKKQPDLNWENPILRSELYQMINTWSQMGVAGFRVDAITFIKKNLSFQSLESDGVDG
                     LVKCTKTVRNQPGIEKMLYELKENTFKKYDCMTVAEAAGISYDQLGDFIGEDGFFSMV
                     FDFKHADLDVASGSEWFKRISWTIPDLRKCILDSQMYVQEHGWAANFIENHDQPRATT
                     KYLLQYERNDDAVKMLGAMYFFLRGTPFIYQGQELGMTNFKRDTINDFNDLSSIDQYY
                     RSIDESYSEEEALEFVNLRSRDNARTPFPWADKENAGFSDTTPWLKVIENYKDINAQS
                     QINNSESILEFYKKMIKLRQDSEYSDCLIYGSIEPIDIESDDIIAYRRQLDLVVIDCY
                     YNFSDKPVEIRVEEKYDIVFQNLDDINLLNKTVRLKPFHAVLLKVR"
     gene            13110..14984
                     /locus_tag="PC545_003919"
     CDS             13110..14984
                     /locus_tag="PC545_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDF2458582.1"
                     /translation="MNKYNKIANELIKIIGEDNIISITHCATRLRVMVKDREIINDKK
                     VEKVDEVKGVFFTSGQYQIILGTGIVNKVYAEVEKMGLKTLSKKEQDELVKNNETGFK
                     KVMRTLADIFVPIIPVIAATGLFLGLKGCLFNDNVLGLFGMSSANIPLYIQTLVSVLT
                     ETAFAFLPAIIVWSAFKVFGGTPVIGLVIGLMLVSPILPNAYSVADPSNEIEAIMAFG
                     FIPIVGCQGSVLTAIVTAFIGANLEKWFRKHMPNVLDLIFTPFFVMLITMLVILLGVG
                     PIMHTIELKMVDIISLLIDLPLGIGGFIIGFTYPLAVITGLHHTYVMIETSLLANTGF
                     NALITLCAMYGFANIGTCLAFMKKSKNNQVKQTAVGAMLSQLFGISEPVLFGIQLRYN
                     LKPLIIMCASSGLGAAILSILHIQSNSYGLAVLPSYLMYIYDGYNLITYLLVSIFVVA
                     FCFIVTCLFGVPKEAINEDEDEELVFNENNENFVSPAKGKIVALENVPDETFSKKMLG
                     DGFAIDIIDGKIVSPISGKLETVVSSGHAFGIKGTNGEVLIHVGIDTVALNGDGFDVA
                     VKQGDMVKQGDVLVNVDLKRIHELGKSTLTMVLFPDGKKVNILDINKDVKIGQRICVE
                     "
BASE COUNT         5709 a         1748 c         2526 g         5216 t
ORIGIN      
        1 attgtaatat ttaaatatta caatagtaag ttttaaatgt ggaatcaatt attatatcac
       61 aaattaaaag gaggacagga tgttattaat agttacctaa ttatcttcgc aataggttta
      121 atcaacataa ttgtactagg tgaagaaaat ataagagata cagctattca tttagtcttg
      181 tttcttataa gtagaaccta gattggagta tattacaaat aatgtctact attttaatag
      241 taggttataa atttaaatat gggagatgat ataaattatg aaaagaaaaa agaattttat
      301 ttggatagct atcttattgg ttggtgtagt tatggtaatg tattatgtag ggaaaaaaca
      361 taacgatata aatcaaaaaa ctgataagaa ttatataaaa tctgaattaa ataagtctga
      421 attaaataaa tccaaaattg aaaacaatga taaaaacaaa aaagaaaaaa ttaatatggt
      481 tgactatagt gattgttttg aaggtattag tggaggagct attttttata atactaaaaa
      541 taaagaatat aatatatata acaaagaatt aattgaaaca agaaggtcac catgttctac
      601 ttttaaaata gtttcaactt taattggttt agaaaaaggg gtaataaact caaaagaatc
      661 agttatggga tatgatggta cagagtatcc aaataaaaat tggaataaaa atcttagttt
      721 agaagaggca tttaaggagt cttgtgtttg gtattacaaa aaattgatag ataaagttga
      781 tgctaaatct gtccaaaaca ttttagatga tttaaagtat gggaattgcg atattagcga
      841 atgggaaggc gatttaaaaa atggaaaggg acatttaaat ggattttggt tagaatcttc
      901 attacaaata tctccaaaag aacaagtgca aacaatggca aaaatatttg agggagatac
      961 aaactttaaa aaagagcata ttaatatttt aagagatatt atgaaaatag atataaatga
     1021 taaaaacata aatgtatatg gaaaaactgg aacaggattt gatgaaaaaa ataaacgtgt
     1081 ggatgcatgg tttgttggta tgctagagcg tgaaggtgac acctattatt ttgctattaa
     1141 atcagatgat tccaataaag aaattacagg gccaaaggta aaggaaattg caattaatat
     1201 aattaagaaa tattatagtg taagggaata aacttaattt tatttgtaaa tattatttac
     1261 ctatagtctg taatatttat taaataattt tactaaattg agtctatata gagcaaaact
     1321 cctttagtac ataaaatact tgctattgat tattaaattg aatacctata gatttaaagt
     1381 taataccttt taaagactat gtaatatatt aaaagtataa ataaaatata ttgcatagtt
     1441 tttgttttat attataattt taaataatgt gcaaaaacta aacaatattt ttaatagtaa
     1501 tttatatttg tattcatgtc actataatgt cattttaggt tgctaaaatt taaccataaa
     1561 ggagatgatt gtatatggaa atattaaaat gtgaaaatct cacaaaaata tatggttcaa
     1621 atcaaaccag agtgacagcc cttaataatg taaatttatc agttcaaaaa ggtgattttg
     1681 tatccattgt aggagcatct ggttctggca aatctacatt gttacatctg cttgggggag
     1741 tagatagacc tacatctgga aagatttatg ttgaagatac agaaatatca agtttaaagg
     1801 aagaagctct tgcagttttt agacgtagaa aagtaggtct tatttatcaa ttttataatc
     1861 ttatacctac ccttgatgtt agaaaaaata tattattacc catgttactt gataaaagaa
     1921 aggtggatga ggatagattc tctgagattg tatctatatt gggattatct gatagattaa
     1981 accatcttcc aagtcaactt tcaggtggac aacaacaaag ggtatctata gctagaagtt
     2041 taatctatag accagctatt ttattagcag atgagccaac tggtaatctt gatagaaaaa
     2101 attcagaaga aattgtagac ttgttaaact tatcaaataa acgatttaat cagacaatac
     2161 ttcttattac tcatgatgag aaaatagcac tagaggcaaa tcgtattgta actatggaag
     2221 atggagtgat tgtctctgaa aaggtggtga agaaatgaat attcttcaag aatacacctt
     2281 agactttgta cgacgcaata aacgtagtag tatagctata atgttagcta tattacttac
     2341 tacaaccatg atgtcttctt tgtgtggact gttgtatacc atgtggacag attttataag
     2401 actttcaatt gagaaagaag gtaactggca tggtgaattg tttgactata cttatggaag
     2461 tgacttacca ttaattgaaa atttttcttc agttgatgat gtaatggtaa aaggacaatg
     2521 gtatgtaggc aaaattaatg atgagaaaag agattatata atttatagaa atgctacatc
     2581 agagtattgg aattctatgc cagaaaagga tactataata gagggtcgtc gaccaaataa
     2641 tgctaatgaa attgcattat caaaacaata ttttgagaat aatccaagtg taaaaattgg
     2701 tgataaaatt actctcccaa taggatatag aatcttaaat ggaaagcctc ttgaacccat
     2761 agccccaaaa tcgaaagggg aaagttttaa aaaaatttca gaggctacat tgacaatagt
     2821 aggaaaggta gatgtttcta cttcatcagt aatacctgca tatacagctc ttggatatct
     2881 tgataaaaca agtataaaac ctaatgatag tataactgtg tacttgcgat ttaaaaatat
     2941 aagagacacg tataaggaac ttccaaaact tgctaaatca ttgggatgga agactaatga
     3001 atatggaaaa tacaatctta aatacaatag taattatctt ttaaagatgt tagtcttttc
     3061 accagaacag aaagcatcta tgagtagtat atcaaaattt tcaactccta ttatgtatct
     3121 tacaatagca atttttacag tagccgtgtt tgtaatggtg atttacaacg cgttttcact
     3181 gtctgcaaat gctagactta ctcagttagg gatattatct agtgttggtg catcaccaaa
     3241 acaaattaaa aggtctgtag tgtttgaagg ctttttactg acaattatac cattaccaat
     3301 aggtttattt ttaggatggt tattgtgtaa taggcttata gtttatgtta attcagttaa
     3361 ttatcatact gatgttccag aagtggtatt tacttatggt attcctgctt ttctgccagc
     3421 agttttactt acaatagtaa cagtctggat ttctgcactt ataccagcac gaaaagtatc
     3481 aaagatatct cctatagaag caatacgaca aggagatagt gttaaaatca aaaagtcgca
     3541 taaatattca ttaggaaaga tatttggaat agaaggagaa cttgcatcaa acgcactacg
     3601 tgctcgtaaa aaatcttatc gtactgcaac aatatcttta acattatctt ttttattact
     3661 tacatgtttt ttgcatataa ttgcaaatca agaaggagca aaagctgttt atggaagtga
     3721 taattataag gaccaaagag atatagcagt ttatttggaa aatagtgaac ctatagagtc
     3781 tgctttcatt gaaaaattag ataatacaga aggtgtaaaa agttcactat attatcaaaa
     3841 aatgtctgct tccacttggc tgactgaaaa agatgcatca aaagaacttg cgaaaaatgg
     3901 aggatttaaa agtatagttt catctaaaaa atactctcct atagaacgtg atggcaagtt
     3961 tcgtataatt tcaaatataa ttgggcttga tgataagagt tttacagaat attgtaggca
     4021 aatttctgta gacccaaagt tattttataa tacaaaaaat ccaacctcaa ttgtatataa
     4081 taaggaggaa gatattaatc gtagtacacg tagaaataaa gtatatatag attatttaaa
     4141 tctttcaata ggagataaca ttaaattaaa tgaaaaagta tatgacgaag ataaagggga
     4201 ttatacttac aacatgaaag tgggagctat tacaagcaca cttcctaaaa tagtagatac
     4261 tagttcaaag tatacattaa tgcaaatagt tccaatgtct atggtacagg aaatatctaa
     4321 aaattatgat gaagtaagta ggttacgttc aaataggctg ataggagtat ttaaagtaaa
     4381 taatcgtgag gacattccta gagtaagaga aaaattagag tccatttata ataaagacta
     4441 tggttcagga gattatagta ttgaggatgt tttagaaagt gaaaaacaaa aggctagtgg
     4501 aataaagacc atgaatttaa tagtaggctt tattacaggt ttattggcta tgattggatt
     4561 atcaaatgta cttgctacag tgtcaggaaa tattagaagt agacgacaag agtttgctat
     4621 gttgcgttct gttggattat ctccagaagg cataaaaaag atgcttattt tggaaggttt
     4681 attcttagga attacaccat tactacttag tattcctgtg caaataggaa tagtgtatgc
     4741 ttttctacgt ataaatgaaa tatattttat agagtattta ccatttgcac ctatatctac
     4801 aataataggt tttactatat tgatactttt tattgtaata gcatcatata tgacaggata
     4861 caagcagttg aagaatgaaa atatagtaga atctataaaa aatgaaacaa tataaacaaa
     4921 atctatttga acttatttaa aggtttcgat ttaagactac ttacaagtat agggatagtt
     4981 aagttgtttg tttaataaaa ccttaaatgt aaatagaagt atattactaa aacaatagat
     5041 aacatagaat atagatatag ctggattata ggtattttac ctataatcca gcattttatg
     5101 tgtaaaattt aattatgaat cttgcaccac cattatggca attttctgca tgtagagtac
     5161 cattttgacg ttctataata gactttgata gagcaagccc tataccaata ctgtccttac
     5221 tagcattttt accacgataa aatctattaa aaatatatgg aagttcttcc tgagcaaacc
     5281 ccttaccatt atcttctatg ataatctcaa catatagagg attttgacta taataagcaa
     5341 ctatatatcc accatcttct atatgctcac tacaattttt aagaatattt aaaagagctt
     5401 ctaaagtcca attaatatcg atttcaaatt ctaccgaagg agaagcctcg atagtaaatg
     5461 tctgatgttt ttctcttaaa gaaccttcta taggctcaat tgcataagta agaagagaat
     5521 gtacatctat aagtttgctt tcaaatatta aagtacttgc atccaatttt gataatgtaa
     5581 gtaaagatga aactagtctc tcaagtcttg agatttgatt tgtaagtctg ttaatataat
     5641 ctatttcttc atctggacaa ttttctgaaa gtaattgtgc cataagtgac atagaagtaa
     5701 ttggagtctt aagttgatga gcaatatctg caagattatc tgaaagagtt tgtctttctt
     5761 ttaaagcaaa ttcttttgaa caacgaagtt ccccaactgt tttatatatc tcatcttcaa
     5821 ggtgagaaaa ttcatcttca catcgtaaaa gtacagactc ttttcctaaa ttcacttctt
     5881 ctaagtattg agtaagattg tttattctac tcagtttttg cttatttgtt ctatgtttga
     5941 ttatatatat aactgtagcc attgttgaaa ttataccaaa cgatatcact agaaaataaa
     6001 aaaagttttt actccaaaat gtacttttag aatatccatg ttttgataaa atttcatttc
     6061 ctgttttaaa atccttatca ctagaatttt tcagtgattt tagtatactt tctgtagact
     6121 taggattgta ctcatatgat gcaccaagag ccttagacag tacattgtat tcattaaaag
     6181 aagttatagt tgttagaatt acactcaata aaaaagttac aattaagcag aatattataa
     6241 aaacaactgt actgttgata tttcttggag ttttcactag ctgtcctcca ttctatatcc
     6301 tatgcctctt aacgttttta tacaaggtgg attttttaat ttttctctaa gacgttttat
     6361 tgtaacagta agagtgttat catttacgaa attaccatct acatcccaga gcatttctag
     6421 taactttgta cgagtcatag ctctattttt attttccata agacttagaa gaagtttata
     6481 ttctcctgct gtcaaaggta cttcaacatt atctaaatat gctttgactt caaccttatt
     6541 caagcttata ttttcacatg taatagtatt gttagtattt gaatgagttc ttctaagaac
     6601 tgcattaatt ctggactgaa gtactgagag caaaaacggt ttaaccacat agtcatctgc
     6661 acccatatct aatccacgta taatgtcttt ttcatcatca cggactgtca aaaatatcac
     6721 tggagtatct tctatacttt taacatattg gcaaaactcg tatccaatac catcaggtag
     6781 atttaaatca agcagaatta aatcaatagt tttatcaaaa ttttttttag cttcagcaat
     6841 cgtattataa caacatacag tgtgattttt cttttcaagg gaagttttta ttccaaatgt
     6901 aattgtatca tcatcttcta aaagaagtat tttagccatt tacttttaat cctttcagta
     6961 atttagttat tccattaact aagttattag ttttatgttt aatatttttt atattagaga
     7021 tagaaaaaat tatcatttaa gtatataaac tttaatttat aaacttaggt ttatgcttta
     7081 attgaatcta ttaaaagagc aagagttgtt tcaataaatt tttcctcatc tatattaaag
     7141 ttgttcaggt atgattttcc tgttacagat agtaaattaa aaaatcctgt aagtgtaaat
     7201 attgatgaat acataagagt aggtatttca atatcatgcc taatacttct atcatttttt
     7261 ccaatttcaa acattccttg tatttcatca aacataaatt tattaaaaga aaaaaacttt
     7321 tctttaaaca gcatatcagt atccgatttt tcagagttta caataccaat cattcccata
     7381 aggcataata gagaaggttc tacagagtaa aatttatgaa aagcaaagta tgaaagtttt
     7441 attttttcaa accctgtttt gccttttgca ctttctattt taacattatc ccaaagtctt
     7501 ttatatcctc ttaagacaac agcaaagaat aaatcttctt tacatgaaaa gtacttgtat
     7561 atggtgcgtt tagtatattc aacttctttt gctaattcat tcatagttgt attatcaaat
     7621 ccattaagac aaaataattt ttctgcttta tcaataatct cattttctct ttgtattttt
     7681 tctttatctc tttttgataa ttctgacata taaattattc ctttctattg ataaaaaagt
     7741 atactgatgg tatacttatg taaatataat acttgtatac ctatagtata ctttaataaa
     7801 aaataaaatt aatatcaagt attataacta tttataactt tattaattaa ttatataggt
     7861 aaaataccaa ataaggggaa tttatttatg aagataaaag aaaatgtatt aatgttagat
     7921 agtactcgtg gctcaaattg ttttattgtg tttgataaag aaattattct tatagacaca
     7981 ggtcttcctt ttatggggaa aaatataatt aaagaattaa aaacacttgg cattaaactt
     8041 acagatatta agcatatttt attaactcat catgatgttg accatatatc taatattaaa
     8101 ttattgaaag aaaaaacagg agcaaaagta tgggctcatg tagaggatat accttttatt
     8161 ataggagaga aggatagacc tgggtttaag aagtttattg gaaaggcaat ttccagaagg
     8221 ctcattaagg atgttgaacc ttatggagag aatatgcaga ttgggaatat aaaaattatc
     8281 catactcctg gtcatactcc agggcatgtc tgcatgattt ttgaagctgt attatttgca
     8341 ggtgatttag tcaaaaataa gaatggaaat atagttcctt atcctaatcc atggaattgg
     8401 gattataaaa agatggtgaa atctataaga gaattggata atttaaaata tgaatggata
     8461 tgtatgtctc atggaactcc ttgtattaaa taataaaaat taacatggat taatagaggc
     8521 atagttgctt gtatgtatga taataattta cagcttcttt ttaatatatc atgtatattt
     8581 gtagctgtaa gcatatttat aacaatactt acaattttgt attttaacat ctataataat
     8641 agttttagct ttgttaacta tgaaatttac ttaaagaagt aaatattcaa gtaatagtaa
     8701 ttattaaata agataaaaag taaatggcag atgaattaaa gtaatttcta ccatggaaaa
     8761 tacttttgag ttagaaaaaa ctatatttta gagtatgaag agatgaagct tccgaagaaa
     8821 gcaatatcta aagtacatgt tcgaaaggat aagtactttt tggtgcctgc ttttagcagg
     8881 ttatttttat tttaataaaa ataaaatcaa atgattattt tatccttatt atttaaaact
     8941 cttccctaga aaaagcaact aatgtatatt aaaataatat ttagggagtg gaaaaaatga
     9001 gattagaatc gttagttaac caaaattatg ataatttaaa tgaaaatgat aaaattatta
     9061 ttaaacaaat attgatttgt caaagagaat accgtaattt ttcgtgtgaa aaaattgcaa
     9121 atgaatgtaa tgtttctaga gctactttat taaggctttg tagaaaaatt ggattagatt
     9181 cattctctga tttaaagtat ttacttaaaa ataatcaact agaatttgtt aataatgata
     9241 ataattttca tgatgtttgc gagaattatc gactattaat gaatgaatta caaaaaatat
     9301 catttcaaga tatttgtaaa ataatatatg atgcagaaac aatttatatt tatgggacag
     9361 gtaatgaaca gaaaagttta gctcaagaaa taaagagaat atttctttca gtaggtaaat
     9421 gtgttattga cttatttgat ataggtgaaa ttgaatttat gagagaatca tttcaaaaaa
     9481 atgatttatt tattatcatt tctttgtctg gagaaacaaa agcaggaatt gaaattgcaa
     9541 aatttatcaa caatcatata catacgattt ctttaactag gcttgataat aatacaattg
     9601 caacactttg tgagtataat ctgtatgttg aaacgcaaaa acttgataca ttacaagcta
     9661 tatcttatga agtcgtagga gcattttatg ctctattaga tttgcttcat atgaattaca
     9721 tagagtatag aaggagaaaa gaatgaaaat tgaaggactt ttcaataaat attataattc
     9781 atttacagca aatgataagt atatctgtga gtgtattctt aatcataaag aagattgtat
     9841 taaactttct atagatgaat ttgcagacaa ataccatata tctacaagtg cactttctcg
     9901 ttttgctcaa aaactacaat tacctggata tagtgaatta agagttatta tacgcttaaa
     9961 tgaaacagaa gtagaatata aaaatcaaag taaacaccaa ttaatggatt gttataataa
    10021 ggtgatagat gatatagaga agaaggattg ttcgattatg tttgaaagaa tgaaaaaggc
    10081 aaatcgtata atcgtctttg gagaaggtta ttctcaggaa agagttgcta aagagatgaa
    10141 aaggattttc ttaccaacag ggaaaattat atatgatgtt tatggttatg atatgattca
    10201 ggcacttaat cattttataa aaacagatga tatgattttc tttatttcat tgaatggaga
    10261 tgcatctaca attattgatt ttgctaaaga aatgagaatg aaaggaattt atatggtttc
    10321 tattacaaaa atgataagta atcctttagc aagattatgt gatgaaaacc tgtatattca
    10381 atctatatct atttctgtag ataaacaact ggactacgat gtaacaacac cttattttat
    10441 tttaattgaa ctgctttata ttaaatacaa gatgtattta gaaagtgaac atattccccg
    10501 aaaatgaaaa tttgtgctaa aaatgaacat attttttaaa agatatcaaa atattgatat
    10561 cttttttttt gaatgctatt atgattttga tagtaagaaa ggaagatgaa attgtacaaa
    10621 ttattaatac ttgatataga tggtacgctt agagatgagg tatatggaat cccagaatct
    10681 gcaaagcatg ctatacgttt atgtcaaaag aatcattgca gtgttgttat ttgtactgga
    10741 agaagtatgg gtacaataca agatgatgtt ttatcattag gagttgatgg atatattgca
    10801 ggaggaggta attacattca atatcatggt gaattacttt ataatcaatc ttttaatcaa
    10861 agattgataa aggaagttgt ttgtttatta aaaaagagag aagttgcttt ttcgattgag
    10921 agtcaggaaa aagttttcat gaatcaaaaa gccaaagaaa tatttgaaac aatgaatcag
    10981 ttgaaaggaa ctaattcttg tataaataaa caacatatcc aagaaaaaat aacatatgaa
    11041 aataacattg aagagtataa gtcacaagat atacataaaa tttgtttatg gtcaaatgaa
    11101 aaggtatttg acgaagtgaa aaatatacta caagataaaa tggaattagc acagagagat
    11161 atttctagtc agtattatga aattattcaa aaagattttc ataagggcaa agcaattaag
    11221 agattacaag aaagattagg agtaactcaa aaagaaacca tatgttttgg tgatggtcaa
    11281 aacgatgttg tcatgtttca agcatcagac gttacaattg caatgaaaaa tagtcatcaa
    11341 caacttaaag atatagcgac atctatatgt gaggacatat tcgataatgg aatttataaa
    11401 gaattaaaaa gaagaaatat catataaaaa ggagtaattt atggaaaaga aatggtggca
    11461 aaaagaaatc gtttatcaaa tttatccaag aagctttcaa gatagtaata atgatggaat
    11521 tggagattta cagggaatta tttctcgttt agattattta aaaaatttag gtattacatc
    11581 attgtggtta tgtccaattt ttaaatcacc tatggacgat aatgggtatg atattagtga
    11641 ttatttggat attgcaagtg aatttggaag tatggaagat ttaaaagagt taattaaaga
    11701 aagtaaagat agaggaatca aaattatatt agatttagtt cttaatcata caagtgatga
    11761 acatccttgg tttcaagaag cattaaaaaa tccagaaagt ccttatcatg attattatat
    11821 ttttaaaaaa ggaaacgatt taccaaataa ctggaggtct gtttttggtg gaagtgtttg
    11881 ggaaaaggta gagaatagag acgaatatta ttttcattct tttggtaaaa aacaacctga
    11941 ccttaattgg gaaaatccaa tattaagaag tgaactttac caaatgatta atacctggtc
    12001 tcaaatggga gtggcaggat ttagagttga tgctattaca ttcatcaaaa agaatttaag
    12061 ttttcaaagt ttagaaagtg atggtgttga tggtcttgtt aaatgtacaa aaacagttag
    12121 aaatcagcct ggaatagaaa aaatgcttta tgaattaaaa gaaaatacgt ttaaaaaata
    12181 tgattgtatg actgttgctg aagcagcagg tattagctat gaccagctag gtgattttat
    12241 tggagaagat ggtttctttt caatggtctt tgactttaaa catgctgact tagatgttgc
    12301 ttctggtagt gagtggttta aaagaatttc atggacaatt ccagatttaa gaaaatgcat
    12361 tttagatagt caaatgtatg ttcaggaaca tggatgggca gctaatttta ttgaaaatca
    12421 tgaccaacca agagctacaa caaagtatct tttgcaatac gaaaggaatg atgatgcagt
    12481 aaaaatgttg ggagcaatgt atttcttttt aagaggaacc ccatttattt atcagggaca
    12541 ggaattagga atgacaaatt ttaaaagaga taccataaat gattttaatg atttatctag
    12601 cattgaccaa tactatcgtt caatagatga aagttactca gaagaagaag cgttagaatt
    12661 tgtgaattta agaagccgag ataatgcaag aacgccattt ccatgggcag ataaagaaaa
    12721 tgcaggtttt tcagatacaa caccatggtt aaaggttatt gaaaattata aagatattaa
    12781 tgcacagagt cagattaaca attcagagag tattttagaa ttttataaaa agatgataaa
    12841 gttgagacaa gatagtgagt attctgattg tttgatttat ggttcaatag aacctattga
    12901 tatagaaagt gatgatatta ttgcgtatag aaggcaatta gatttggtag ttattgattg
    12961 ttattataat ttttctgata aacctgtaga aataagagta gaagaaaaat atgatattgt
    13021 attccaaaat ttagatgata taaatctatt aaataaaaca gtgcgtttga aaccatttca
    13081 tgcagtatta ttgaaagtga ggtagaaaaa tgaataaata taataaaata gcaaatgagc
    13141 ttattaaaat tattggtgaa gataatatta tttctattac acattgtgcc acaagattaa
    13201 gagtgatggt taaagataga gaaattatta atgataagaa agttgaaaaa gttgatgagg
    13261 ttaaaggggt tttctttact tctggacagt atcaaattat tttaggaaca ggaatagtta
    13321 ataaagttta tgcagaagtt gaaaaaatgg gattaaaaac tttatcaaaa aaagaacaag
    13381 atgaattagt taaaaataat gaaactggat ttaagaaagt tatgagaact ctagcagata
    13441 tatttgttcc tattattcca gttattgcag caacaggatt attcttggga ttaaagggtt
    13501 gtttgtttaa tgataatgta cttggattat ttggaatgtc ttctgcaaat attccattgt
    13561 atattcagac acttgtttcg gttcttacag aaacagcatt tgctttttta ccagcaatta
    13621 ttgtatggtc agcatttaaa gtatttggag gaacacctgt tattggactt gttattggac
    13681 ttatgttagt ttccccaata cttcctaatg catattctgt tgctgaccca tcaaatgaaa
    13741 ttgaagcaat tatggcattt ggatttattc ctattgtagg ttgtcaagga agtgttttaa
    13801 cagctattgt tacagcattt attggagcta atcttgaaaa atggttccgt aaacatatgc
    13861 ctaatgtatt agaccttatt ttcacaccat tttttgttat gctaataaca atgttagtta
    13921 ttttgttagg tgtaggccct ataatgcata caatagaatt aaaaatggtt gatatcatca
    13981 gcttattgat tgatttgcct ttaggaattg gaggatttat tattggattt acatatccat
    14041 tagctgttat tactggctta catcatactt atgtaatgat tgaaacatca ctattagcaa
    14101 atacaggatt taatgccctt attactcttt gtgcaatgta tggatttgcg aatattggaa
    14161 catgtctagc atttatgaaa aaatctaaaa ataatcaagt taaacaaact gcggttggag
    14221 caatgctatc tcaacttttt ggtataagtg agcctgtttt atttgggatt cagcttcgat
    14281 ataatttaaa gcctttgatt attatgtgtg catcttcagg tttaggagca gcaatattat
    14341 caattttaca tattcaatct aattcatatg gattagcagt tttaccttct tatttaatgt
    14401 atatctatga tggatataat ttaatcacat acttattagt atcaatattt gtggttgcat
    14461 tctgctttat tgtaacatgt ttatttggag tgcctaaaga agctataaat gaagatgaag
    14521 acgaagaatt agtgtttaat gaaaataatg aaaactttgt gtctccagct aaggggaaaa
    14581 ttgttgcatt agaaaatgta ccagatgaaa cattttctaa aaaaatgtta ggtgatggtt
    14641 ttgctattga tattatagat ggaaaaattg tttctcctat ttcaggtaaa ttagaaacag
    14701 tggtttcaag tggacatgca tttgggatta aaggaactaa tggagaagta ttaattcatg
    14761 ttggaattga tacagttgca ttaaatggag atggctttga tgttgctgtt aaacaaggag
    14821 atatggttaa acagggagac gttcttgtta atgttgattt aaagagaata catgaattag
    14881 gaaagtctac tttgacaatg gtattgtttc cagatggtaa gaaggtaaat attttggata
    14941 taaacaagga tgtaaaaata ggtcaaagaa tatgtgttga gtagtaggtg atatgtaatg
    15001 actacaaaag aagattatca agagtaattc aatcttttta aagtatgaga aataattcat
    15061 aaactctaaa acaattaaag aggttgtttc aaaatagaga ttgtattgca cccaaattgt
    15121 tgaaatgatg aaaatatatt taacttcata caaaaaatta aatagtggca acaattgagg
    15181 atgtgaggga gagttgcca
//
LOCUS       DAMJVI010000287         4804 bp    DNA     linear   BCT 12-JAN-2023
DEFINITION  TPA_asm: Clostridioides difficile strain SH660 isolate JH272
            SAMN32366125-rid16592293.guided.178, whole genome shotgun sequence.
ACCESSION   DAMJVI010000287 DAMJVI010000000
VERSION     DAMJVI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN32366125
            Sequence Read Archive: SRR22863037
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001560893.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001560893.1
            Genome Coverage       :: 262x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/05/2023 01:40:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,927
            CDSs (total)                      :: 3,840
            Genes (coding)                    :: 3,790
            CDSs (with protein)               :: 3,790
            Genes (RNA)                       :: 87
            rRNAs                             :: 7, 2, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 7 (5S)
            partial rRNAs                     :: 2, 5 (16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 24 of 50
            Pseudo Genes (incomplete)         :: 22 of 50
            Pseudo Genes (internal stop)      :: 14 of 50
            Pseudo Genes (multiple problems)  :: 7 of 50
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN32366125-rid16592293.guided.178"
                     /strain="SH660"
                     /isolate="JH272"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:1496"
                     /geo_loc_name="USA"
                     /collection_date="2017-03"
     gene            complement(157..384)
                     /locus_tag="PC545_003920"
     CDS             complement(157..384)
                     /locus_tag="PC545_003920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458583.1"
                     /translation="MIVACNALTQLLYSWLATVFSNASIYWGASNSIFLTPRGNLPKR
                     SCINGASTDCLKKDKKSIVSNSSLRPILVQA"
     gene            612..1538
                     /locus_tag="PC545_003921"
     CDS             612..1538
                     /locus_tag="PC545_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDF2458584.1"
                     /translation="MSDILSRITNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSGIDPEEIHALESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARVIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNETVEIVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            1552..2001
                     /locus_tag="PC545_003922"
     CDS             1552..2001
                     /locus_tag="PC545_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDF2458585.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPHPYREQNARDFV
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAELGYWLARNYWGKGIATN
                     AVKEICKIAFEKYNLNKIHANVYSYNRGSSKVLEKCGFKIEEYCHNS"
     gene            2361..2768
                     /locus_tag="PC545_003923"
     CDS             2361..2768
                     /locus_tag="PC545_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDF2458586.1"
                     /translation="MVDEKTIEHQFDSFCKTVLRNYARDIYDENKRRNDYLVSLESLS
                     LAELSKLSILDDYDSNYICMVSYDYNIRIEDVLMAQAIGKLTKRKQDIILLSFFLNMT
                     NADIATLMDLAENTIHYHKTNALKELKELMEEH"
     gene            2770..3000
                     /locus_tag="PC545_003924"
     CDS             2770..3000
                     /locus_tag="PC545_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDF2458587.1"
                     /translation="MKPTKEVLPFTIILLAVHGDVNAINGILKHFEHYIIRLSQKTLF
                     DEYGNPYIHVEEETKRMLGTKLITAILRFNLS"
     gene            3390..3593
                     /locus_tag="PC545_003925"
     CDS             3390..3593
                     /locus_tag="PC545_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HDF2458588.1"
                     /translation="MNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLADENLDSGWVI
                     MNSNRIQIKRKQFEKIVDTLDFI"
     gene            3671..4024
                     /locus_tag="PC545_003926"
     CDS             3671..4024
                     /locus_tag="PC545_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase DNA-binding domain-containing protein"
                     /protein_id="HDF2458589.1"
                     /translation="MSTEKRRDNKDRILRTGESQRKDGRYAYKYVYSFGKPQFVYSWK
                     LVPTDKPPKGKRDDISLRDKEKAIQNDLDDSIDTIGKKMTVCQLYAKKNGLRKNIRLN
                     TKKGRHYLMKILNKE"
     gene            4096..4728
                     /locus_tag="PC545_003927"
     CDS             4096..4728
                     /locus_tag="PC545_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDF2458590.1"
                     /translation="MTIPSSNKVYPRSNDYQTVYLKNVITRENIEVGDYTIYNDFYND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHSMKSKSTYTFPIFYEEW
                     KLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVNIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNDTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDKLV"
BASE COUNT         1780 a          674 c          825 g         1525 t
ORIGIN      
        1 ttttttttta ttttttcatt atataatact cctatgcata attatgttgg attgatagct
       61 aataaaagaa agtgagaggt gttatactaa aaaagaagca tacttcccaa ctccagactt
      121 acagtggatt gaagatagat tttggatttg ggttcattac gcttgcacta agattggacg
      181 tagagaacta tttgaaacaa ttgatttttt atctttttta agacaatcag tacttgcccc
      241 attaatacag cttctcttag gtaaattacc tcgtggagtt agaaaaattg aattggacgc
      301 accccaatat attgaagcat tagaaaagac ggttgctaac catgaatata aaagctgtgt
      361 taatgcattg caggctacta tcaacctgta tcttgagtta agagaggaat tatcacaaag
      421 tcctattgtc cgaaaggttg aagctgaaag ggttgcatta cagtattttc aagatttaat
      481 caaataaacc caaagtaaaa aaatattgaa tagtaaatat tcagtgtttt ttcatttttg
      541 gtcaaatgac ctagattagt tgttgtaacc attctataat tattttgaaa gtaacagtat
      601 ggaggataac tatgagtgat attttaagta gaattacaaa taaaattggg gataagaata
      661 tagttgataa gttatcagct ctatcaaaat cagatttgaa ctcacttcta ttagaagtgt
      721 ttgatagaca agcaaatact ttaactgcaa ctgatattct aaagtcatac caactaaata
      781 gatttactat ccctagcggt atagatccag aagaaattca tgctttggag tcaaaattat
      841 tgagaaaagc attcaatatg gatatcaaga cgattatgct ttctccctct gctccattag
      901 gtagctgtag cgtttttggc tccgtagatc aatataatgt agtaagtgct ctacgaggga
      961 cagaaacact tgcagacccc tcaaatatgt tggcaataat tattgctgat aaattaaaaa
     1021 gaaaagaaga aaataatacc atacctatcc acatggctac tactgctagg gtaataagag
     1081 cacaaaattt tgtaggtaaa ggtttatatt cacattttgg cttatattgt atggtttcat
     1141 ctggtataga tacagggtct tacacttgtg aaaaaatgtt gttagaaaag cacttaaact
     1201 attataaaga tatattaagt gaaatagaaa atgttcgttt ctctattatt ctacgcaaaa
     1261 gaaacggata caaagataat gacggatttt tcgacagaat ggttgaaact atcaaactta
     1321 tttttcctaa tatagagtta tcaattcata atgatgatgt tgataataat tattataaag
     1381 gtatcaattt caaattatcg gtcaaacaag gaaatgaaac cgttgaaata gttgacggtg
     1441 gatttgttga ctggacttat caaatgcttg gaaataaaaa agaacgctgt ttgataagtg
     1501 gtattggctt agaaagattt cttgtagctg tttcataaaa ggagaagaaa aatggaactt
     1561 atagagtgga attatgctta tattaccgat ttagttgagt ttgcgaatga taaagaaatt
     1621 gcaaagaatc ttcgtgacaa cttcccccac ccttataggg agcaaaacgc tagagatttt
     1681 gttagttttt gtttggcaac tccagatgtt aaacaactta gctatgctat tttttatcaa
     1741 gaaagggcta ttggaagtat cagcctaaca tttgaagaag atacctctat taataaaaat
     1801 gctgaattag gttactggct tgctaggaac tattggggca aagggatagc aacaaatgca
     1861 gtaaaagaaa tatgcaaaat tgcctttgaa aaatacaacc ttaacaaaat tcatgctaat
     1921 gtatattcct acaatagagg gtcaagtaaa gtattggaaa aatgtggttt taagattgaa
     1981 gaatactgtc acaattcata ataaagaata gaaacatgat tgattgccat gtatatagtt
     2041 taattcgacc gcaatagcat ttattagaat taacctactt tttaataaca gaataagttt
     2101 ttcccctcta ttaatttctt atgcatcttt gtatttttat gaaaagctag ccaatgcata
     2161 tttccataca tcaatatggt ggtggcttac aaccaatata aattaaatac gttttaaaga
     2221 atacacattt ttgccctata ggcagagtgt gtattttttt ctcttttttt tgaaatttat
     2281 cgaagattta acgccctccc accgtgatag gagtaagcaa ggttatttaa ctccttgcga
     2341 tggaaagagg gtgaaatctt atggttgatg aaaagacaat tgaacatcaa ttcgacagtt
     2401 tctgtaaaac agttttgcgt aactatgcta gagatattta tgacgaaaat aaacgcagaa
     2461 atgattattt ggtatctctc gaatctttgt cacttgcaga attaagtaaa ttgagtattt
     2521 tagatgatta tgattctaac tacatctgta tggtttctta cgactacaac atacgaattg
     2581 aagatgtatt aatggcacaa gcaatcggaa aacttacaaa aagaaaacaa gatataattc
     2641 tactttcatt ctttctcaac atgacaaatg ctgacatagc aacattgatg gatttagcag
     2701 aaaataccat acattatcac aaaacaaatg cactaaaaga gttgaaagaa ctcatggagg
     2761 aacattgaaa tgaaacctac taaagaagta ttacctttta caattatttt acttgctgta
     2821 catggcgatg taaatgcaat aaacggaata cttaaacatt ttgaacacta tatcattcgt
     2881 ctttcacaga aaactttatt tgatgaatac ggaaatccct atattcatgt agaagaagaa
     2941 acaaagcgaa tgctaggaac aaaactaatc actgctattt tgagatttaa tttgtcttaa
     3001 tctcgccctc tctccatgta tcaagctatt tggtacatgg agaatcacta attatttgtc
     3061 tataaaagcc cataagctat tgtgattttt tatagacaaa tcaatgccga ctgttctttg
     3121 acaactgaat aaagtagata gatacgtttg tgatacagat agccaacaaa tactatacgc
     3181 catgacctta atcaagagag ctactatata gtgatttgtg aataaccttt ggaacggttg
     3241 ttttgccgta acctgtacca cagtataatg atacacctgt atgacacggt tctgcccata
     3301 agaatgggat tggttaaaat accaatggag cttaccaaag ccatctatct acttctgaac
     3361 tacgaacata gaaaggagtg atacattcta tgaacaatac tgatgtacct atttgggaaa
     3421 aatatacctt gacgattgaa gaagcgtcaa aatactttcg tattggagag aaaaaattac
     3481 gtaaacttgc tgatgaaaac cttgattctg gctgggtcat tatgaatagc aatcgcattc
     3541 aaataaagag aaaacaattt gagaaaatcg ttgatacact tgactttatc taatgaaatt
     3601 gagccttata tgttataata ttcatatgac gtatcaagct caattcggtt attgagagga
     3661 gcttcttatc atgtcaactg aaaaaagacg agataataaa gatagaattt taagaactgg
     3721 agagagccag agaaaagatg gaagatacgc atataagtat gtatattcat ttggtaaacc
     3781 acaatttgtt tactcttgga aacttgtacc tactgataaa cccccaaagg gaaaacgtga
     3841 tgatatatca ttaagggata aggaaaaagc aatacaaaat gaccttgatg atagcattga
     3901 cactattggt aagaaaatga cagtttgtca gttatacgct aaaaagaatg gactaagaaa
     3961 gaatatccga cttaacacta aaaaaggacg ccattatctt atgaagattc ttaacaagga
     4021 gtgattctgt gaagagatgt attgtagtag tggaatagtc aaaaacataa tatatattaa
     4081 aggagagatt taaatatgac tattccaagt tcaaataagg tatatccaag aagtaatgat
     4141 tatcaaactg tatatcttaa aaatgtaata acaagggaaa atatagaagt tggagattac
     4201 acaatatata atgactttta taatgaccca agagattttg agaaaaacaa tgtattgtat
     4261 cattacccta taaacaatga taaattaata attggtaagt tttgctcaat agcatgtaat
     4321 gcaaaatttc taatgactag tggaaatcac tcaatgaaat caaaatccac atatactttt
     4381 cccatctttt atgaagaatg gaaactcgat atcaaccata taacggacgc ttgggacaat
     4441 aagggtaaca ttataattgg aaatgatgtg tggataggtt atgattctat aattatgtca
     4501 ggtgtaaata ttggtgatgg tgcaattatt ggaacaagag cagtagttac taatgacgtt
     4561 ccaccgtatt ctattgttgg aggagtacct gcaaaaatta taaaaaagag atttaataat
     4621 gatacaattt caaaattatt agaaattaaa tggtgggatt ggccatataa aaaaattcaa
     4681 tcaaacatta agcatattca atcaggaaat attgataaat tagtttaata ttttataatt
     4741 tatttatatt aaactttact ttaataatca ttttataaga gctttttcaa aatttgtata
     4801 ttat
//
